LOCUS       DBNHBY010000001         9039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_744366, whole genome shotgun sequence.
ACCESSION   DBNHBY010000001 DBNHBY010000000
VERSION     DBNHBY010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_744366"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="AC16HB_00005"
     CDS             complement(<1..935)
                     /locus_tag="AC16HB_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5605198.1"
                     /translation="MPAVESVDAVILGTGQAGKPLAQALARAGIRTVVVEREHVGGTC
                     INVGCTPNKTMVASARVAYLARRGADYGVNTGAVAVDLRRVIARKRAIVERFRNYGTQ
                     RLQSTPNLELVFGHARFVDGRAVEVRLRDGGARLFAAPKIVINTGGRPVMPGIAGLTD
                     VPTLDSTTIMELDELPRHLVVLGGGFIGLEFAQMFRRFGSEVTVLEGMARLAPRDDED
                     VSEALRKVLEEDGIRIECDARVERVDGRAGDIRVHYAQGGTTTSVRGSHLLVSVGRRP
                     NTDDLNLAAAGVVTERGYVTVDATLATNVPGIYAVG"
     gene            complement(978..2156)
                     /locus_tag="AC16HB_00010"
     CDS             complement(978..2156)
                     /locus_tag="AC16HB_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5605199.1"
                     /translation="MARRASNKKAAARGNGAASAESVVTRPPGQIVLVLQGGGALGAY
                     QIGVYQALHEAGIEPDWVIGTSIGAINAALIAGNDPDDRLRQMHAFWDVLEWRSPFSL
                     VPTGPGVLNPLASLSILTQGVPGFFKPNPASWWGLTTPLGVDEAAFYDTGPLRETLQR
                     HCNFGRANRGEPRLTVGAVNARTGAMKYFDSREEPLRVEHVMASGALPPAFPAIRIDG
                     EPYWDGGVYSNTPIEAVLDDNPRRSSTIFAIQLWNPTGEEPESIWEVFGRQKEIQYAS
                     RADTHIARQKQIHRLRHVINQLGQQLPDGKRNSTAVRELLSWGCRTNMHIVHLHAPRL
                     AAEDHTKDIDFTPAGIRARCEAGYRDTRDAIAEQPWNCAIDPIEGVFVHDHDSMAEML
                     "
     gene            complement(2256..2741)
                     /locus_tag="AC16HB_00015"
     CDS             complement(2256..2741)
                     /locus_tag="AC16HB_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR5605200.1"
                     /translation="MFKQLVRTTFALLIAVGALAVSPARAADDGIVRVKSAYSTDETI
                     ARLRQDIAAKGIMFFTEIDQARLAANAGVTLPAIKRSTLLIFGNPPLGTLFINGRPEA
                     GIDWPVRLLVQEDTSGNVYAVYTDFGWIARRHGISGTSEGPFKTASGLIASITSSVAA
                     K"
     gene            complement(2752..3117)
                     /locus_tag="AC16HB_00020"
     CDS             complement(2752..3117)
                     /locus_tag="AC16HB_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIR5605201.1"
                     /translation="MSVREFIEISGTAAEIAGVVLIVGGLLLASGRCLLPRTGERAER
                     LARYRQDLGRAILLGLEVLVAADIVRTVAFTPTMESVQVLAVIVAIRTFLSWSLALEL
                     EGHWPWQRAARDPQPSSTQ"
     gene            complement(3130..4371)
                     /locus_tag="AC16HB_00025"
     CDS             complement(3130..4371)
                     /locus_tag="AC16HB_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605202.1"
                     /translation="MSTHADVCAIAGAPPDTPATALLRRLLIGLTAFLTLVDLFATQA
                     ILPSLAAAYRVTPAAMGFAVNASTIGMAVSGLVVAFFSRRIDRRRGIFVSLALLAIPT
                     ALLAVAPSLPIFTALRIAQGLCMSAAFTLMLAYLAEHCSEREVAAAFAAYITGNVASN
                     LFGRLMSAALADTLGIAGNFYVFAALNLAGAALVHFTLSRTPSMLMPMSSARSPWSVY
                     AEHLRNPALRTAFALGFLILFVFLGVFTYVNFVLRRPPLALAPMTLGFVYFVFAPSMF
                     TTPVAGKLVARFGVRPACIGGLAVAIIGLPLLVLPSLPAVLAGMVLVAAGTFFAQATT
                     TGFVGRAATTDRASANGIYLASYFLGGLVGAAVLGQVFDGLGWGAVVAGVAVALGVSV
                     LLAAHLKLPAQAAAAPAHAMA"
     gene            complement(4458..4967)
                     /locus_tag="AC16HB_00030"
     CDS             complement(4458..4967)
                     /locus_tag="AC16HB_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5605203.1"
                     /translation="MSTNSKQQPSWRKRAVAGLIAGAFSLAAGAALAGECPADKRVGD
                     GQGAKDAGKGAKGVTDVVINTLDVANEPSVMVKGRLFRGRKLEIQPGGVVPWHSHADR
                     PALLLIATGEISEYASTCTVPIVHKAGEITAEKAPTSHWWQNHGKTPVVIWAFDLFRT
                     DDKKNEHMM"
     gene            complement(5245..6345)
                     /locus_tag="AC16HB_00035"
     CDS             complement(5245..6345)
                     /locus_tag="AC16HB_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR5605204.1"
                     /translation="MSLNYQQAGVDYAKIDPLKILAQRAAAATAGNLARHGLTEVAAS
                     RGESAYVVDCGDFYLASITECLGTKALVADAMRALTGRTYYDRIAQDTLAMAINDIIT
                     VGATPVSVHAYWAAGGSAWFDDADRARDLVEGWKAACDRCGVAWGGGETPALAGVIAE
                     GRIDLAASCVGIVRPKSRLTLGDRLAEGDAIVLLESSGIHANGISLARKLAERLPDGY
                     ATRLADGTAFGEALLVPTLLYSPVTEALAAAGIVPHYCANITGHGWRKLMRHPGSFTY
                     RITQLPPVPPVLAFIQRETGSDAREAYGNLNMGAGFALFVGANDARRTVDIAARCGVR
                     GFIAGRVESGAKQVVIEPLALVFGGDDLHVRA"
     gene            6400..7467
                     /locus_tag="AC16HB_00040"
     CDS             6400..7467
                     /locus_tag="AC16HB_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5605205.1"
                     /translation="MSVFTPQRVQAGLWIGVGIALLVLLYVLAPVLTPFAAAFILAYL
                     LVPGVDWLARHRVPRAAAVLLMILFALLALTALLLILVPVLQKEIVALKEQFPALINK
                     LNADVAPRINELFGTSIRFDSQTLKELLAERVGSQDDIVAKIIEHARAGGVALLGIVG
                     TLILIPVVLFYALLDYHSFTDRVGTLIPRRWHHASTGMLNDVDRLLANFLRGQLLVMG
                     ILAVYYSIALTIAGFQAALPIGILTGLLIFIPYVGFALGLMLALLTAILQFGSWYGLI
                     AVAIVYGVGQLIESFFLTPKLVGESIGLHPLAVIFALLAFGQVFGFFGVLLALPASAA
                     LLVAVQRIKEAYLASEFYNQP"
     gene            7464..8147
                     /gene="hda"
                     /locus_tag="AC16HB_00045"
     CDS             7464..8147
                     /gene="hda"
                     /locus_tag="AC16HB_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HIR5605206.1"
                     /translation="MSTQQLEGGNAARQLALDLLQPLQPSLDNFVAGRNREALDALRR
                     AASLAAPERIIYLWGEPGCGRTHLLAALAAAPRAYVWSPATAPERPGLALVDDVDTLD
                     GMAQIALFTRLNAVRERADAACVIAGPLPPAQLALRDDLRTRLAWGLAYQLHALTDVE
                     KAGALAAHAASRGLALPPDVTGWMLSHLPRDMRTLVAALDVLDAYALARKKPLTLPLA
                     KEALAAPSN"
     gene            8170..8850
                     /locus_tag="AC16HB_00050"
     CDS             8170..8850
                     /locus_tag="AC16HB_00050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5605207.1"
                     /translation="MKLALFDLDRTLLPIDSADTWSHFLVQEGGLDDEGYGARISAFA
                     ESYAANRFDVDAYQDFQMELLARFPRAQLEHWRARFIREHVLPHLRPEAQALIDDHRR
                     DDFVAVLVTGTNAFVTRPIAAAFGLEHLLAVEPEEVDGRFTGRYVGTHTHQHGKVRAV
                     EAFVAAHGAKLDDCEDSVFYSDSINDLPLLERVRRPVATNPEPRLRAVAVARGWPTLE
                     LFSPAQAR"
     gene            8847..>9039
                     /locus_tag="AC16HB_00055"
     CDS             8847..>9039
                     /locus_tag="AC16HB_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR5605208.1"
                     /translation="MIKRFVEKVQTLFTRKPRVKKEPKHYAVAEHRIDRALVPRSAMR
                     VCETLQKAGCDAYIVGGAVR"
BASE COUNT         1379 a         3113 c         3149 g         1398 t
ORIGIN      
        1 ccgaccgcgt agatgccggg cacgttggtc gcgagcgtcg catcgacggt cacgtagccg
       61 cgctcggtga cgacgccggc ggcggcgagg ttcaaatcgt cggtgttcgg ccggcgcccg
      121 accgacacca gcagatgcga gccgcgcacc gacgtcgtcg tgccgccctg cgcgtagtga
      181 acgcggatgt cgcccgcccg cccgtcgacg cgctcgaccc gcgcgtcgca ctcgatgcgg
      241 atgccgtcct cctcgagcac cttgcgcagc gcctcgctca cgtcctcgtc gtcgcgcggc
      301 gcgagccgcg ccatgccttc gagcaccgtc acttcgctcc cgaaacggcg gaacatctgc
      361 gcgaattcga gcccgatgaa gccgccgccg agcacgacca gatggcgcgg cagctcgtcg
      421 agttccatga tggtcgtcga atcgagcgtc ggcacgtccg tcaggcccgc gatcccgggc
      481 atgaccggcc ggccgccggt gttgatcacg atcttcggcg cggcgaacag gcgcgcgccg
      541 ccgtcgcgca gccgcacttc gaccgccctg ccgtcgacga agcgcgcgtg cccgaacacc
      601 agctccaggt tgggcgtgct ctgcagccgc tgggtgccgt aattgcggaa acgctcgacg
      661 atcgcacgct tgcgcgcgat cacgcgtctg aggtcgacgg cgaccgcgcc ggtgttcacg
      721 ccgtaatccg cgccgcggcg cgcaaggtaa gcgacgcgcg cgctcgcgac catcgtcttg
      781 ttcggcgtgc agccgacgtt gatgcaggtg ccgccgacgt gctcgcgctc gacgaccacg
      841 gtccggatgc cggcccttgc cagcgcctgc gccaggggct tcccggcctg gccggtaccc
      901 aggatcactg catcgacgga ttcaacggcg ggcatgggtc ctccctcggg ctggttggtg
      961 ccctcacgaa tcggcggcta cagcatctct gccatcgaat cgtgatcgtg gacgaacacg
     1021 ccttcgatcg gatcgatcgc acagttccac ggctgctcgg cgatcgcgtc ccgggtatcg
     1081 cgatagccgg cctcgcagcg cgcgcggatg ccggccggcg tgaagtcgat gtccttggtg
     1141 tggtcctccg ccgcgaggcg cggcgcgtgc aggtgcacga tgtgcatgtt cgtccggcag
     1201 ccccacgaca gcagttcgcg caccgcggtg ctgttgcgct tgccgtcggg cagctgctgt
     1261 ccgagctggt tgatgacgtg gcgcagccgg tggatctgct tctgtcgcgc gatatgcgtg
     1321 tcggcgcggc tcgcgtactg gatctccttc tgccggccga agacctccca gatcgattcg
     1381 ggctcctcgc cggtggggtt ccacagctgg atcgcgaaga tcgtcgagct ccggcgcggg
     1441 ttgtcgtcga gcaccgcctc gatcggcgtg ttcgagtaca cgccgccgtc ccagtacggc
     1501 tcgccgtcga tgcggatcgc cgggaatgcc ggcggcagcg cgcccgaggc catcacgtgc
     1561 tcgacacgca gcggctcctc gcggctgtcg aaatacttca ttgcgccggt gcgcgcgttg
     1621 accgcgccga cggtcaaccg gggctcgccg cggttcgcgc gaccgaagtt gcagtgccgc
     1681 tgcagcgttt cacgcagcgg cccggtgtcg tagaacgcgg cctcgtcgac gccgagtggc
     1741 gtggtcagtc cccaccacga cgccgggttc ggcttgaaga agcccggcac accctgcgtc
     1801 aggatcgaga gactggcgag cgggttcagc acgcccggtc cggtcggcac gagcgaaaac
     1861 ggcgaccgcc actccagcac gtcccagaac gcgtgcatct gccgcaggcg atcgtcgggg
     1921 tcgttgccgg cgatcaacgc ggcgttgatc gcgccgattg acgtgccgat cacccagtcg
     1981 ggctcgatgc ccgcctcgtg caacgcctgg tacacgccga tctggtacgc gccgagcgcg
     2041 ccgccgccct gcagcaccag cacgatctgg cccggcgggc gcgtcaccac ggattcggcg
     2101 gacgcggcgc cgttgccgcg cgccgcagcc ttcttgttcg atgcacggcg tgccatgctg
     2161 ttctccggta acggtttcgg tgggcggccg tcggccggct gccggttagt ctgcgcgcgc
     2221 cgtcaaacct ttcaaccgcg cacacgacgc cggaatcact tcgcggcgac gctcgacgtg
     2281 atcgacgcga tcaggccact ggcggtcttg aacgggcctt ccgaggtgcc gctgatgccg
     2341 tggcggcgcg cgatccagcc gaaatcggtg tagaccgcgt acacgttgcc gctcgtgtct
     2401 tcctgcacca gcagccgtac cggccagtcg atgccggcct cgggccggcc gttgatgaac
     2461 agcgtcccca gcggcgggtt gccgaagatc agcagcgtgg agcgcttgat cgccggcagc
     2521 gtgacgccgg cgttggcggc gagccgggcc tggtcgatct ccgtgaagaa catgatgccc
     2581 ttggcggcga tgtcctgcct cagccgcgcg atggtttcgt cggtcgaata cgcgctcttc
     2641 acgcgcacga tgccgtcgtc ggcggcgcgc gccggcgaca cggcgagcgc accgacggcg
     2701 atcagcaggg cgaatgtggt gcgaacgagt tgcttgaaca tggttgcctc ctcattgggt
     2761 tgatgacggt tggggatcgc gcgcggcgcg ctgccacggc cagtggcctt cgagttcgag
     2821 cgcgaggctc cagctcagga acgtgcggat ggcgacgatc accgccagca cctgcacgct
     2881 ttccatcgtt ggcgtgaacg cgacggtgcg cacgatgtcg gccgccacca gcacctcgag
     2941 cccgagcagg atcgcgcggc cgaggtcctg ccggtagcgc gcaagccgct cggcgcgttc
     3001 gccggtacgc ggcaacagac agcgcccgct cgccagcagc aggccgccga cgatcagcac
     3061 gacgcccgcg atctcggccg cggtgccgga gatctcgatg aactcgcgca cggacatctc
     3121 ggctcccgct cacgccatcg cgtgcgccgg cgccgccgca gcttgcgcgg gcagcttcag
     3181 gtgcgcggcg agcagaaccg acacgccgag cgcgaccgcc acgccggcca cgaccgcgcc
     3241 ccagcccaga ccatcgaaga cctggcccag cacggcggca ccaaccagac cgccgaggaa
     3301 atagctggca aggtagatgc cgttggccga ggcgcggtcc gtggtggcgg cacgaccgac
     3361 gaagccggtc gtcgtagctt gggcaaagaa ggttccggca gcgaccagca ccatgccggc
     3421 cagtacggcg ggcaggctcg gcagcaccag cagcggcagc ccgatgatcg ccaccgcgag
     3481 cccgccgatg cacgccgggc gcacgccgaa tcgtgcaacc agcttgcccg ccaccggcgt
     3541 ggtgaacatc gacggcgcga acacgaagta gacgaagccg agcgtcatcg gcgcaagcgc
     3601 gagcggcggc cgccgcagca cgaagttcac gtaggtgaac acgccgagga acacgaacag
     3661 gatcaggaac ccgagcgcga acgcggtgcg cagcgcggga ttgcgcaggt gctcggcgta
     3721 caccgaccac ggcgatcgag cgctgctcat cggcatcagc atcgacggcg tgcgcgaaag
     3781 cgtgaagtgc accaacgcgg cgccggcgag attgagcgcc gcgaaaacgt agaagttgcc
     3841 ggcgatgccg agcgtgtcgg cgagcgcggc cgacatcagc cggccgaaca ggttgctcgc
     3901 gacgttgccg gtgatgtaag ccgcgaacgc ggcagcgacc tcgcgctcgc tgcagtgctc
     3961 ggcgaggtag gccagcatca gcgtgaacgc ggccgacatg cacaagccct gtgcaatgcg
     4021 cagcgcggtg aagatcggca gactcggcgc gaccgccagc agcgcggtcg ggatcgcgag
     4081 cagcgccagg ctgacgaaga tgccgcgccg gcggtcgatg cggcggctga agaacgcgac
     4141 gacgaggccg gagaccgcca tgccgatcgt gctcgcgttg accgcgaatc ccatcgcggc
     4201 aggcgtgacg cgatacgcgg cggcaagcga cggcaggatg gcctgcgtgg cgaagagatc
     4261 caccagcgtc aggaacgcgg tcaggccgat cagcagccgt cgcagcagcg cggtcgcagg
     4321 tgtatcgggc ggtgcgcccg cgatggcgca cacatcggca tgcgtggaca tggaacggct
     4381 cttccgttgg cgggaatcgc gcggcgagca ccgcggggca gcgctcgccg cgaaccaggg
     4441 cgcgcgtgat tgcgccgtta catcatgtgt tcgttcttct tgtcgtcggt gcgaaacagg
     4501 tcgaacgccc agatcacgac cggcgtcttg ccgtggttct gccaccaatg cgaggtgggc
     4561 gccttctcgg cggtgatctc cccggccttg tgcacgatcg gcaccgtgca ggtgctcgcg
     4621 tactcggaga tttcgccggt ggcgatcagc agcagcgccg gccgatcggc atggctgtgc
     4681 cagggcacga cgccgccggg ctggatttcg agcttgcggc cgcggaacag ccggcccttg
     4741 accatcacgc tcggctcgtt ggcgacatcg agggtgttga tcacgacatc ggtgacaccc
     4801 ttggcgcctt tgccggcgtc cttcgcgccc tggccgtctc cgacgcgctt gtcggccggg
     4861 cattcgccgg ccagcgccgc accggcggcc aacgagaagg cgccggcgat cagaccggcg
     4921 acggcgcgct tgcgccacga gggttgctgc ttcgagttgg tgctcatcac gattctcctt
     4981 ccgcgagaac tcccgcagcc accgcggccg cggatattgg tcgcagcgcc agcccgtgcg
     5041 ggcgaattcg cgtgggatcg acaggaagct gctctcacga gcccgtggct tgagcgtccc
     5101 ccgatcggcc gttacgcggc cagcgctgcg ttgcgtgatt ggtcacgcga gacgcacaaa
     5161 cctttcagca gcgcgcggaa gaaacagcga tgcgaagcgc gtaaggaatc gcgcgccggt
     5221 ggcgacgcgc ggcacgcggg aatgtcaggc gcgcacgtgc aggtcgtcgc cgccgaacac
     5281 gagcgcgagc ggctcgatga cgacctgctt cgcacccgac tcgacgcgac cggcgatgaa
     5341 gccgcgcacg ccgcagcgcg ccgcgatgtc gaccgtgcgc cgggcgtcgt tcgcgccgac
     5401 gaacagcgcg aagccggcgc ccatgttgag gttgccgtac gcttcgcgcg cgtcggaccc
     5461 ggtctcgcgc tggatgaacg cgagcacggg cggcaccggc ggcaactgcg tgatgcgata
     5521 ggtgaacgaa ccggggtggc gcatcagctt gcgccagcca tggccggtga tgttggcgca
     5581 gtagtgcggc acgatgcccg cggccgcgag cgcctcggtg accggcgagt acagcagggt
     5641 cggcaccagc agcgcctcac cgaacgccgt gccgtccgcg agccgcgtcg cgtagccgtc
     5701 gggcagccgc tcggccagct tgcgcgcgag cgaaatacca ttggcatgga tgccgctcga
     5761 ttcgagcagc acgatggcat cgccctccgc gagccgatcg ccgagcgtca gccgcgactt
     5821 cggccgcacg atgccgacgc acgacgccgc gagatcgatc cgcccttccg caatcacgcc
     5881 ggcgagcgcg ggcgtctcgc cgccacccca cgcgacgcca cagcggtcgc aggcggcctt
     5941 ccagccttcg accaggtcgc gcgcgcgatc cgcgtcgtcg aaccaggcgc tgccgccggc
     6001 ggcccaatac gcgtgcaccg acaccggcgt cgcgccgacg gtgatgatgt cgttgatcgc
     6061 catcgccagc gtgtcctgcg cgatacggtc gtagtacgtg cgccccgtga gcgcgcgcat
     6121 cgcgtccgcg acgagcgcct tggtgccgag gcattcggtg atcgacgcca gatagaaatc
     6181 gccgcagtcg accacgtagg cggattcgcc gcgcgaggcg gcgacctcgg tcaggccgtg
     6241 gcgcgcgaga ttgcccgccg tcgccgccgc ggcgcgctgc gcgaggatct tcagcggatc
     6301 gatcttcgcg tagtcgacgc cggcttgctg gtagttgagc gacattgaat ttggcgttgg
     6361 cgtctagact acgcggcggt tttcaacgga ttctagtcaa tgagcgtctt caccccgcag
     6421 cgcgtgcagg ccggtctgtg gatcggcgtc ggcatcgcac tgctggtcct gctctacgtg
     6481 ctggcaccgg tgctcacgcc gttcgcggcg gcgttcatcc tcgcctatct gctggtaccg
     6541 ggggtagact ggctcgcgcg ccaccgggtg ccgcgcgcgg cggcggtgct gctgatgatc
     6601 ctgttcgcgc tgctggcgct caccgcgctg ctgctgatcc tggtgccggt gctgcagaag
     6661 gagatcgtgg cgctgaagga gcagttcccc gcactgatca acaagctgaa cgccgacgtc
     6721 gcgccgcgca tcaacgagtt gttcggcacc tcgatccgat tcgacagcca gacgttgaag
     6781 gaactgctgg cagagcgcgt cggcagccag gacgacatcg ttgccaagat catcgagcac
     6841 gcgcgcgccg gcggtgtggc gctgctcggc atcgtcggca cgctgatcct gatcccggtc
     6901 gtgctgttct acgcgctgct cgactatcac agcttcaccg accgcgtcgg cacgctgatc
     6961 ccgcgccgct ggcatcacgc ctcgaccggc atgctgaacg acgtcgaccg gctgctcgcg
     7021 aatttcctgc gcgggcagct gttggtgatg ggcatcctcg cggtgtacta ctcgatcgcg
     7081 ctgacgatcg ccggattcca ggccgcgctg ccgatcggca tcctgaccgg gctgctgatc
     7141 ttcattccgt acgtcggctt cgcgctcggg ctgatgctgg cgctgctgac cgcgatcctg
     7201 cagttcggca gctggtacgg tctgatcgcg gtggcgatcg tctacggcgt cgggcaactg
     7261 atcgagagct tcttcctgac gcccaagctg gtcggcgaga gcatcggcct gcatccgctc
     7321 gcggtgatct tcgcgctgct ggcgttcggc caggtgttcg gcttctttgg cgtgctgctg
     7381 gcgctgccgg caagcgcggc gctgctggtc gcggtacagc ggatcaagga agcgtatctc
     7441 gcgagcgagt tctacaacca gccgtgagca cgcagcaact cgaaggcggg aacgctgcgc
     7501 gccagctcgc gttggatctg ctgcagccgc tgcagccttc gctcgacaat ttcgtggcgg
     7561 gccgcaaccg cgaggcgctc gatgccttgc ggcgcgcggc gtcgcttgcg gcgccggagc
     7621 ggatcatcta cctgtggggc gagccgggct gcgggcgcac gcacctgctg gcggcgctcg
     7681 ccgccgcgcc gcgcgcgtat gtctggtcgc ccgccactgc gcccgagcgg ccggggctgg
     7741 cgctggtcga cgacgtcgac acgctggacg gcatggccca gatcgcgctc ttcacgcgtc
     7801 tcaatgctgt acgcgagcgt gccgacgccg cgtgcgtgat cgccgggccg ttgccgccgg
     7861 cgcagctcgc gttgcgcgat gacctgcgta cgcggctcgc gtggggactc gcgtaccagc
     7921 tgcacgcgct gaccgacgtc gaaaaggccg gtgcgctggc ggcgcacgcc gcatcgcgcg
     7981 ggttggcgct gccgcccgac gtgaccggct ggatgctctc gcacctgccg cgtgacatgc
     8041 gcacgctggt ggccgcgctc gatgtgctcg acgcgtatgc gctggcgcgg aagaagccgc
     8101 tgacgttgcc gctcgcgaaa gaagcgctcg cggcgccgtc gaactaacgg aacgtagaat
     8161 cgcagcagga tgaagctggc gctgttcgat ctcgaccgca cgctgctgcc gatcgacagc
     8221 gcggacacgt ggtcgcactt cctggtgcag gaaggcggcc tcgatgacga aggctatggc
     8281 gcgcgcatca gcgccttcgc cgaaagctac gcggcgaacc ggttcgacgt cgacgcctat
     8341 caggacttcc agatggaact gctggcgcgc tttccgcgcg cgcaactcga gcactggcgc
     8401 gcgcggttca tccgcgagca cgtgctgccg cacctgcggc ccgaggcgca ggcgctgatc
     8461 gacgatcacc ggcgcgacga cttcgtcgcg gtattggtga ccgggaccaa cgcattcgtc
     8521 acgcggccga tcgccgccgc gttcggcctc gagcatctgc tggcggtgga gccggaggaa
     8581 gtcgacggcc gcttcaccgg ccgctacgtc ggcacgcaca cgcaccagca cggcaaggtg
     8641 cgcgcggtcg aggcgttcgt cgccgcgcac ggcgcgaagc tcgacgactg cgaggacagc
     8701 gtcttctaca gcgactcgat caacgacctg ccgctgctcg aacgcgtgcg gcgcccggtg
     8761 gcgaccaacc ccgagccgcg gctgcgcgcg gtggccgtcg cgcgcggctg gccgacgctg
     8821 gaactgttct cccccgcgca ggcccgatga tcaagcgctt cgtcgagaag gtgcagacgc
     8881 tgttcacgcg caagccgcgc gtgaagaagg aaccgaagca ctacgcggtc gccgagcacc
     8941 ggatcgaccg cgcgctggtg ccgcggtcgg cgatgcgcgt gtgcgagaca ctgcagaagg
     9001 cgggctgcga tgcctacatc gtcggcggcg ccgtgcgcg
//
LOCUS       DBNHBY010000002         4196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1624134, whole genome shotgun sequence.
ACCESSION   DBNHBY010000002 DBNHBY010000000
VERSION     DBNHBY010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1624134"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16HB_00060"
     CDS             <1..525
                     /locus_tag="AC16HB_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5605209.1"
                     /translation="VEWGTLRQAFTEGKTAMMWHTTGNLTAVKDSAKFPFGVAMLPAS
                     KQRGSPTGGGNFYLFKKTTPEERKAALAFVKWMTDPERAADWSIATGYVATRPDAYET
                     AKLKTYAAGFPQAVVARDQFPYATPELSTFQTGRVRKLLDDAIQAALTGQKSPADALK
                     SAQQEADRLLKPYR"
     gene            522..1385
                     /locus_tag="AC16HB_00065"
     CDS             522..1385
                     /locus_tag="AC16HB_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5605210.1"
                     /translation="MKHVYGWLLLTPATILLVAFTHYPAAATFIDSFFSSGTAIRPSR
                     FVGLANYAALADDPVFWQVLGNNFRFALGTIPTAIALALVMALWVNDKLPARGLVRLA
                     YFTPTILPMIAVANLWLFFYTPQIGLIDQVGALFGAPSHNWLGDQHTVLGATMLMVIW
                     KEAGFFMIFYLAALQSLPPELEEAAKIEGASRWYFFWRVTFPLLMPTTLFVLVNATIN
                     SFKLVDHLFILTKGGPDNASSLLLYYIYQVAFSYFDTAYASTLTVVLLTLLAGFAVLQ
                     FLLIEKRVHYR"
     gene            1382..2200
                     /locus_tag="AC16HB_00070"
     CDS             1382..2200
                     /locus_tag="AC16HB_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5605211.1"
                     /translation="MRVLGTRAPLLETGAAWLLALLWLAPLAYAFWAAFHPAELATRF
                     QLLAPLTLGNFAKAWTFAPFPRHFLNTVILVTMILAAQFVLCTLAGYAFARFDFAGRD
                     LAFGLVLVQLMVMPEVLIVENYRIMSRLGLVDTIVGIGLPYMASAFGIFLLRQTFKTV
                     PRELEDAARVEGCNFLGVLWRVYVPLARPTYLAYGLVSVSYHWNNFLWPLVITNSVET
                     RPLTVGLAIFGAPESGVDWAVISAGTLTAVAPLLVAFLLFQRQFIQSFMQAGIK"
     gene            2203..3054
                     /locus_tag="AC16HB_00075"
     CDS             2203..3054
                     /locus_tag="AC16HB_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5605212.1"
                     /translation="MKLITWNIQWCCGVDGRVDPARIVEHARALADFDVLCLQEVAAN
                     FPALAGSAGENQFEILARLLPGYAAIPGAGVDALAPDGSRRAFGNLILSRLPVLQALR
                     IQLPWPPDPDVKSSMPRLLIDATVEAPFGPLRVMTTHLEYYSARQRAAQVESIRSHHA
                     EACDRSRRPGARGKPESPYHSYPQPAAAILTADFNFRPDDPLYARLQAPFPDGAPGFV
                     DAWTVAHPGAPHVHTNGVYDRVQWPAPHACDFVFVTDDLRARVRAVEVDSVTQASDHQ
                     PVLIALA"
     gene            3051..4070
                     /locus_tag="AC16HB_00080"
     CDS             3051..4070
                     /locus_tag="AC16HB_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5605213.1"
                     /translation="MTPPSKVVRTDRELECPRIDAALRARGVDLVLLPEGVTEDELIE
                     AARDAVLLLMCYTPITARVIAAARALRGIVKYGVGIDAIDIDAATARRIPVVNVPEYA
                     EETVAEGAFALLLALAKKLVTLQVSMQTVGWAWPTSKWLGIDIAGKTVGLVGVGRIGR
                     SFARMAGGFRATVLGYDPHVGAAAMQRAGVRKCESLHAMLAESDFVSIHCVLNGDTRH
                     LIGARELAAMKRSAFLINVSRGATVDEAALLRALTDRAIAGAALDVYSQEPLSKQGHP
                     LSALFAMDNVILLPHLTFYTHEAMQRLEDETLARCFEILDGRPVLVKSHDPRLTSQRF
                     GVVFR"
     gene            complement(4063..>4196)
                     /locus_tag="AC16HB_00085"
     CDS             complement(4063..>4196)
                     /locus_tag="AC16HB_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR5605214.1"
                     /translation="IGMALDTALNLFQWNRVAMVLFSIFAVVVLAEIVVTQIRKRII"
BASE COUNT          627 a         1456 c         1389 g          724 t
ORIGIN      
        1 gtcgaatggg gcacgctgcg gcaggccttc accgagggca agaccgcgat gatgtggcac
       61 acgaccggca acctcaccgc ggtgaaggac agcgcgaaat tcccgttcgg cgtggcgatg
      121 ctgccggcgt cgaagcagcg cggctcgccg accggcggcg gcaacttcta tctgttcaag
      181 aagaccacgc ccgaggagcg caaggccgcg ctggcgttcg tcaaatggat gacggatccc
      241 gagcgcgcgg ccgactggag catcgccacc ggttacgtcg ccacgcggcc cgatgcgtac
      301 gagacggcca agctcaagac ctatgcggca ggcttcccgc aggcagtggt cgcgcgcgac
      361 cagttcccgt acgcgacacc ggagctgtcg accttccaga ccgggcgcgt gcgcaagctc
      421 ctcgatgacg cgatccaggc cgcgctgacc ggccagaaat cgcccgccga cgcgctgaaa
      481 tccgcgcaac aggaggccga ccggctgctc aagccctatc gatgaagcac gtctacggct
      541 ggctgctgct gacgcctgcc acgatcctgc tcgtcgcgtt cacgcactac ccggccgcgg
      601 cgaccttcat cgacagcttc ttttccagcg ggacggcgat ccggccgtcc cgcttcgtcg
      661 ggctggccaa ctacgctgcg ctcgccgacg acccggtgtt ctggcaggta ctgggcaaca
      721 acttccgctt cgcgctcggc accattccca ccgcgatcgc gctcgcgctc gtgatggcgc
      781 tgtgggtcaa cgacaagctg cccgcgcgcg ggctggtgcg gctcgcgtac ttcacgccaa
      841 ccatcctgcc gatgatcgcg gtggcgaacc tctggctctt cttctacacg ccgcagatcg
      901 ggctcatcga ccaggtgggc gcgctgttcg gcgcgccgag ccacaactgg ctcggcgacc
      961 agcacacggt gctcggcgcc accatgctga tggtgatctg gaaggaagcg ggcttcttca
     1021 tgatcttcta cctcgcggcg ctgcaatcgc tgccgccgga gctggaggaa gcagcgaaga
     1081 tcgaaggcgc ttcgcgctgg tacttcttct ggcgcgtgac gtttccgctg ctgatgccga
     1141 ccacgctctt cgtgctggtc aacgcgacga tcaactcgtt caagctggtc gaccacctgt
     1201 tcatcctgac caagggcggg ccggacaacg cctccagcct gctcctgtac tacatctacc
     1261 aggtcgcgtt cagctacttc gacaccgcgt acgcctcgac gctgacggtc gtgctgctca
     1321 cgctgctggc cggctttgcg gtgctgcagt tcctgctgat cgagaaacgg gtgcactacc
     1381 ggtgagagtc ctcggcaccc gcgcgccgct gctggaaacg ggcgccgcct ggctgctggc
     1441 gctgctgtgg ctggcacccc tcgcgtacgc gttctgggcc gcgtttcacc cggccgagct
     1501 ggcgacacgg ttccagctcc tggcgccgct cacgctcggc aacttcgcca aggcatggac
     1561 cttcgcgccg ttcccgcgcc acttcctgaa caccgtcatc ctggtgacga tgatcctcgc
     1621 cgcgcagttc gtgctgtgca cgctcgccgg ctacgcgttc gcgcgctttg acttcgcggg
     1681 gcgcgacctc gcgttcggcc tcgtcctggt gcaattgatg gtcatgcccg aggtgctcat
     1741 cgtcgagaac taccgcatca tgagccggct cggcctggtc gacaccatcg tcggcatcgg
     1801 gctgccgtac atggcgagcg cgttcggcat cttcctgctg cggcagacgt tcaagaccgt
     1861 accgcgcgag ctggaggacg cggcgcgcgt cgagggctgc aatttcctcg gcgtgctgtg
     1921 gcgggtgtac gtgccgctgg cgcggccgac ctacctcgct tatgggctgg tgtcggtcag
     1981 ctatcactgg aacaacttcc tgtggccgct ggtgatcacc aattcggtcg agacgcggcc
     2041 gctgacggtc gggctggcga tcttcggcgc acccgagtcg ggcgtggact gggccgtgat
     2101 cagcgccggc acgctcaccg cggtggcgcc gctgctggtc gcgttcctgc tgttccagcg
     2161 ccagttcatc cagtctttca tgcaggcggg gatcaagtga cgctgaagct catcacctgg
     2221 aatatccagt ggtgctgcgg cgtcgacggg cgcgtcgatc ccgcgcgtat cgtcgagcat
     2281 gcgcgcgcgc tggccgactt cgacgtgctg tgcctgcagg aagtggcggc gaacttcccg
     2341 gcgctcgccg gcagcgccgg cgaaaatcag ttcgagatcc tcgccaggct tctgcccggc
     2401 tacgccgcga ttccgggcgc cggcgtcgat gcgctcgcgc ccgatggctc gcggcgcgcg
     2461 ttcggcaatc tgatcctgtc gcgcctgccg gtgctgcagg cgctgcgcat tcagctcccg
     2521 tggccacccg atccggacgt gaagagcagc atgcctcggc tgctgatcga tgcgacggtc
     2581 gaggcgcctt tcgggccgct gcgcgtgatg acgacgcacc tggagtacta ctcggcgcgg
     2641 cagcgcgcgg cacaagtcga aagcatccgt tcgcatcatg cagaggcgtg cgaccgctcg
     2701 cggcggcccg gcgcacgggg caagccggag tcgccgtatc actcgtatcc gcagccggcc
     2761 gcggcgatcc tcaccgccga tttcaacttc cgccccgacg atccgctgta cgcgcgcctg
     2821 caggctccct tccccgacgg cgcgcccggc ttcgtcgatg cgtggacggt cgcgcatccg
     2881 ggcgcgccgc atgtgcacac caatggcgtg tacgaccgcg tgcaatggcc cgcgccccac
     2941 gcctgcgatt tcgtcttcgt caccgacgac ctgcgcgcgc gcgtgcgggc cgtcgaggtc
     3001 gattccgtca cgcaggcatc cgaccaccag ccggtgctga tcgcgctcgc atgacgccgc
     3061 cctccaaggt cgtgcgcacc gaccgcgaac tggagtgccc gcgcatcgac gcggcgctgc
     3121 gtgcgcgcgg cgtggatctc gtgctgctgc ccgagggtgt caccgaagac gagctgatcg
     3181 aagctgcgcg cgacgcggtg ctgctgttga tgtgctacac gccgatcacc gcgcgcgtga
     3241 tcgcggccgc gcgcgctttg cgcggcatcg tcaagtacgg cgtcggcatc gacgcgatcg
     3301 acatcgacgc cgccacggcg cggcgcattc cggtcgtcaa cgtgcccgag tacgcggagg
     3361 aaacagtcgc ggaaggcgcg tttgcgctgc tgctcgcgct ggcgaagaag ctcgtcacgc
     3421 tgcaggtctc gatgcagacc gtcggctggg cgtggcccac ctcgaagtgg ctcggcatcg
     3481 acatcgccgg caagacggtc gggctggtcg gcgtcggccg catcggccgc agcttcgcgc
     3541 ggatggcggg cgggttccgc gcgaccgtgc tcggctacga cccgcacgtc ggcgccgccg
     3601 ccatgcagcg cgccggcgtg cgcaagtgcg agagcctgca cgcgatgctg gccgaaagcg
     3661 acttcgtgtc gatccactgc gtgctgaacg gcgacacgcg ccacctgatc ggcgcacgcg
     3721 aactggcagc gatgaagcgc tccgcgttcc tgatcaacgt ctcgcgcggc gcgaccgtcg
     3781 acgaggccgc gctgctgcgc gcgctgacgg atcgcgcgat tgccggcgcg gcgctcgacg
     3841 tctacagcca ggagccattg tcgaagcagg gccatccgct gagcgcgctg tttgcgatgg
     3901 acaacgtgat cctgctgccg catctgacct tctacaccca cgaggcgatg cagcggctgg
     3961 aagacgaaac gctcgcgcgc tgcttcgaga tcctcgacgg ccggcccgtg ctggtgaagt
     4021 cgcatgatcc gcggctgacg tcgcagcggt tcggggtcgt gttcagatga tccgcttgcg
     4081 gatctgcgtg acgacgatct cggcgagcac caccacggcg aagatcgaga acagcaccat
     4141 cgccacgcgg ttccactgga agaggttgag tgccgtgtcg agcgccatgc cgatgc
//
LOCUS       DBNHBY010000003         4302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_135429, whole genome shotgun sequence.
ACCESSION   DBNHBY010000003 DBNHBY010000000
VERSION     DBNHBY010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_135429"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="AC16HB_00090"
     CDS             <1..1441
                     /locus_tag="AC16HB_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5605215.1"
                     /translation="RLQTLLGLRLAMTAASLPPAAAWAWPPPAIAEGFARAITALELP
                     RYSEHRPELADTVLQSLLLHTDLIVDYLDRGASAAAAVAMAIEEFSADWKDRCGALDE
                     LMDVFGDLPKHTRWDLLRGLLAGASWQEVVRIRRLLERLPELARIIRGLGRAHATDEP
                     DPARRSSAPQFERAEALVQRTRSVHIPQLPGETRGIERSGRVARMLPAEALLLGHPRL
                     RLVWHARRAERTLLTYEDDDRLEEEVFEPAPVWRPSPRPEPERRLEMGPIILCIDTSG
                     SMQGGAEAVAKAAVLEAVRTAHAQRRACHLFAFSGPDEIVERRLGLDMQGIEDLAEFL
                     GQTFRGGTDICGPLARSLDLVQDEAMRLADLLIASDGEFGATRELAERLARCKAEQGL
                     RVQAILIGDRETIGLLELADDIFWVRDWRRYGSSQSDSPVHSKSLTAMYFPGTLRTPE
                     NRDRTVSGAHASAVVRGQSRSDAGEEDGR"
     gene            1738..2163
                     /locus_tag="AC16HB_00095"
     CDS             1738..2163
                     /locus_tag="AC16HB_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605216.1"
                     /translation="MPRNRPLRNHTSTRSLPRAWRWFGVVARTLHLAAVILLGAALLG
                     SPPYLSVAVIGGALLASGALLFTLDQWKFSTHAFQLAGAGQIAKLALVVWMTLDPATA
                     PPLFWIIVVWSSVLSHAPASFRHMDLRDLLGIRRQPVDH"
     gene            complement(2157..2906)
                     /locus_tag="AC16HB_00100"
     CDS             complement(2157..2906)
                     /locus_tag="AC16HB_00100"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR5605217.1"
                     /translation="MDDRQLTRYSRHILLPQIDIAGQERLLAGSVLLIGAGGLGAPAA
                     LYLAAAGVGRLTICDGDRVDLTNLQRQVIHREAGIGLNKAESAFRAIAEINPDCRVQA
                     IPRRVAREDLRQLARECDVLVDASDNFATRHAANAVCVELGKPLVSGAAIRFSGQIAV
                     FDRRRAGAACYECLIPSGTEAGDDACAVMGVFAPLTGVIGTLQAAETIRLLAGLPGAV
                     DGELVLFDILSMDFTRVPVARNPNCPVCGRQ"
     gene            complement(2922..3476)
                     /locus_tag="AC16HB_00105"
     CDS             complement(2922..3476)
                     /locus_tag="AC16HB_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605218.1"
                     /translation="MTTFDPDLPEGKGWLTKAQARRQLLDPLEPISGRESAALLDAPN
                     RALDRDMVAPYEVSSHDNCATDGDALRAADLTPDAPTRLAVVGAAVAGIASSGSIGPG
                     QAARVMTVADVAMLRDDVTAIHAAEVEIPPRQPARQKVRRAEFLRGVLRPMSEANGLI
                     ALTEDQGSVAARDRVPVQVCAGLV"
     gene            3637..4107
                     /locus_tag="AC16HB_00110"
     CDS             3637..4107
                     /locus_tag="AC16HB_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5605219.1"
                     /translation="MMKSLYLDPKKCTGCLQCEMACSYENTGAFNPAKSRIKVFNFEH
                     EGRKVPYTCTQCAEAWCMMACPVEAIKVDAVTGAKIVLDATCVGCKVCTIACPFGTIN
                     YVADTGKVQKCDLCGGDPACAAACPTGAITYVDADWTGLDKMRQWAARANTTVM"
     gene            4118..>4302
                     /locus_tag="AC16HB_00115"
     CDS             4118..>4302
                     /locus_tag="AC16HB_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5605220.1"
                     /translation="MGWTRKLLRVDLTAGTCKSEPLNMKWADEYLGSRGLASKYLFEE
                     IDPKVDALAPGNKLIMAT"
BASE COUNT          674 a         1492 c         1472 g          664 t
ORIGIN      
        1 acgcttgcag acgctgctgg ggctgcggct ggcgatgaca gccgcatcgc tgccgcccgc
       61 cgcagcgtgg gcatggccgc cgccggcgat cgccgaagga ttcgcgcgtg ccatcacggc
      121 gctggagttg ccgcgctaca gcgagcaccg gccggaactg gccgacacgg tgctgcagag
      181 cctgctgctg cacaccgacc tcatcgtcga ctacctcgat cgcggcgcga gtgcagccgc
      241 ggccgtcgcg atggcgatcg aggagttcag cgcggactgg aaggaccgct gcggcgcgct
      301 cgacgaactg atggacgtgt tcggcgacct gccgaagcac acgcgctggg acctcctgcg
      361 cgggctgctc gctggcgcga gctggcagga agtcgtgcgc atccgccggc tgctggagcg
      421 gctgccggaa ctcgcgcgca tcatccgcgg ccttggccgc gcgcacgcga ccgacgagcc
      481 tgacccggcc cgccgcagca gcgcgccgca gttcgagcgc gccgaggcgc tggtgcagcg
      541 aacgcgcagc gtgcacatcc cgcagctgcc gggcgagacg cgcggcatcg agcgctcggg
      601 ccgggtcgcg cgcatgctgc ccgccgaggc gttgctgctc ggccatccgc gcctgcgcct
      661 ggtgtggcac gcgcgccgcg ccgaacgcac gctgctcaca tacgaggacg acgaccgcct
      721 cgaggaagaa gtgttcgagc ctgcgcccgt gtggcggccg agcccgcggc ccgagcccga
      781 gcggcgcctg gagatgggac cgatcattct atgcattgac acgtcgggct cgatgcaggg
      841 cggtgctgaa gcggtcgcca aggcggccgt gctcgaagcg gtgcgtaccg cgcacgcgca
      901 gcgacgcgcc tgccacctgt tcgcgttcag cggccccgac gagatcgtcg aacgcaggct
      961 cggcctcgac atgcagggca tcgaggactt ggccgaattc ctcggccaga cattccgcgg
     1021 cggcactgac atctgcggcc cgctggcgcg cagccttgac ctcgtgcagg acgaagcgat
     1081 gcggctggcg gacctgctga tcgcctccga tggcgagttc ggcgccacgc gcgagctggc
     1141 cgagcggctc gcacgctgca aggccgagca ggggctgcgc gtgcaagcga tcctgatcgg
     1201 cgaccgcgag acgatcggcc tgctggaact cgccgacgac atcttctggg tgcgcgactg
     1261 gcgccgctac ggcagctcgc agtccgactc gccggtgcac tcgaagagcc tgaccgcgat
     1321 gtatttcccc ggcacgctgc gcacgccgga gaaccgcgac cgcaccgtat cgggcgcgca
     1381 cgcctcggcg gtggtgcgcg gccagtcgcg ctccgacgcc ggcgaggaag acggacgatg
     1441 accattgccg ccgaactgct gtcactgctc gcgcgcgcct cctgaagcct cgcacccgct
     1501 ccgttgcgat cgcgccgtcg ccgcgcacgt gtccaacccg tcgcacaaca tccgcggccg
     1561 gtcctgaatc gcttcggtac cggtggccaa cgggcggtcc tggaggtctg cgatcgcgtc
     1621 aaccggagga aatcgggtgc cggcctgttc tggcacgacg cgccggccac gtccagcacc
     1681 ggtgacggct gaagtggacc gcatggcaac acgcgccgcc gtcggctata agggcgcgtg
     1741 ccccgcaacc gtccgcttcg gaatcacacg tccacgcgtt cgctgccgcg cgcttggcgc
     1801 tggttcggcg tcgtcgcgcg cacgctgcac cttgcggcgg tgatcctgct cggcgcggcg
     1861 ctgctcggat cgccgccgta cttatccgtc gcagtgatcg gcggcgcgct gctcgcctcc
     1921 ggcgcgttgc tgttcacgct cgaccagtgg aagttcagca cgcatgcgtt tcagctcgct
     1981 ggcgcgggac agatcgccaa gctcgcgctg gtcgtgtgga tgacgcttga tccggcgacg
     2041 gcaccgccgc tgttctggat catcgtcgta tggtcatcgg tgctgtcaca tgcgccggcg
     2101 agctttcggc acatggacct gcgcgacctg ctgggcattc gccgccagcc cgtcgatcac
     2161 tgacgcccgc acaccgggca gttcggattg cgcgccaccg gaacgcgggt gaagtccatg
     2221 ctcagaatgt cgaacaacac gagttcgccg tcgaccgcgc cgggcaggcc cgcgagcagc
     2281 cggatcgtct cggcggcctg cagcgtgccg atgacgccag tcagcggcgc gaacacgccc
     2341 atcactgcgc acgcatcgtc gccggcctcg gtgcctgaag ggatcaggca ctcgtagcac
     2401 gccgcaccgg cgcgccggcg atcgaacacc gcgatctgtc ccgagaaacg gatcgccgcg
     2461 ccgctgacca gcggcttgcc gagttcgacg cacacggcgt tggcggcgtg gcgggtcgca
     2521 aagttgtcgc tggcgtcgac cagcacgtcg cactcgcgcg ccagctgccg cagatcttcg
     2581 cgcgccaccc ttcgcgggat cgcctgaacg cggcaatcgg ggttgatctc tgcaatggca
     2641 cgaaacgccg attcggcctt gttcaggcca atgcccgctt cgcgatggat cacctgccgc
     2701 tgcagattgg tgaggtcgac ccggtcgccg tcgcagatcg tcagtcggcc cacgccggcg
     2761 gcggccaggt acaacgccgc gggcgcgccc aggccgccgg caccgatcag caggacgctt
     2821 ccggcgagca gccgctcctg gcccgcgatg tcgatctgcg gcaagaggat gtggcggctg
     2881 tagcgcgtga gttgccggtc gtccatcgca cctcgtcgcg gttacaccag ccccgcgcac
     2941 acttgcacgg gaacgcggtc cctggcggcc acgctgccct ggtcctcggt cagcgcgatc
     3001 aagccgttgg cttcggacat cgggcgcagc acgccgcgca ggaactcggc ccggcggact
     3061 ttctgccgcg ccggctgacg gggcggaatc tccacttcgg cagcgtggat cgcggtgacg
     3121 tcgtctcgga gcatcgcgac gtccgcgacg gtcatgacac gcgccgcctg gccggggccg
     3181 atgctgccgc tggaggcgat gcccgcgact gcagcgccga cgaccgcgag tcgcgtcggc
     3241 gcatccggcg tgaggtcggc cgcgcgcaac gcatcgccgt cggtggcgca gttgtcgtgc
     3301 gacgaaactt cgtacggcgc caccatgtcg cggtccagcg cgcgattcgg tgcgtcgagc
     3361 agcgccgcgc tttcccggcc tgagatcggt tccagaggat cgagcagttg gcggcgcgcc
     3421 tgcgccttcg tgagccagcc cttgccttcc ggcagatcag ggtcgaatgt ggtcatggcg
     3481 catgcggtgg aagcacgccg cacatgggat cgtgcgcagg gacttccgaa agcatagact
     3541 tcgcagtact tttcagcgtt gccgctgtgt ggctcagttc catctcacgc ggctctccga
     3601 caccaacacc attcatcgac ggaggagtac cggctcatga tgaagtccct gtatctcgac
     3661 ccgaagaagt gtacgggctg tctgcaatgc gagatggcgt gctcctacga gaacaccggg
     3721 gcattcaacc ccgcgaaatc gcgcatcaag gtgttcaact tcgaacacga aggccgcaag
     3781 gtgccttaca cctgcactca gtgcgccgag gcctggtgca tgatggcctg cccggtggag
     3841 gcgatcaagg tcgatgccgt tacgggcgcc aagatcgtgc tggacgcgac ctgcgtcggc
     3901 tgcaaggtgt gcacgatcgc ctgcccgttc ggcacgatca actacgtggc cgacaccggc
     3961 aaggtgcaga aatgcgatct gtgcggcggc gatccggcct gcgccgctgc gtgcccgacg
     4021 ggcgcgatca cctacgtcga cgccgactgg accgggctcg acaagatgcg gcagtgggcc
     4081 gcgcgtgcca acaccacggt catgtgagga gacgacgatg ggatggacga gaaagcttct
     4141 gcgcgttgac ctgaccgccg gcacctgcaa gtccgagccg ctgaacatga aatgggccga
     4201 cgaatatctc ggctcccgcg ggctggccag caagtacctg ttcgaggaga tcgatccgaa
     4261 ggtcgatgcc ctcgcaccgg gcaacaaact gatcatggcc ac
//
LOCUS       DBNHBY010000004         9051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_338533, whole genome shotgun sequence.
ACCESSION   DBNHBY010000004 DBNHBY010000000
VERSION     DBNHBY010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_338533"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC16HB_00120"
     CDS             <1..345
                     /locus_tag="AC16HB_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5605221.1"
                     /translation="IHGNNLGDLPNLMRLAYESDGKLTYHGGEATLELGGRRLFMIHY
                     PHLARGMATTGDFDAVLCGHSHEAKIEQVTNVRGGRTPLVNPGTVAGLGAPATMVLGD
                     LATLSFEVQALA"
     gene            342..1118
                     /locus_tag="AC16HB_00125"
     CDS             342..1118
                     /locus_tag="AC16HB_00125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5605222.1"
                     /translation="MRAAIVILLLLSFGAVAQPLETYSVSTGKYDVPVHVAKPTRAGP
                     FAPVLYVHARRGLEEEDRAHMRELAQAGFLVLAPDWQAANMIERWPDRHYPESEDDVE
                     AGLNALLARKDVCKGPVGVVGISRGPYFAIRLAAKRPQDIAAIVSYYGHFQNPNAPEP
                     QQLYSVAPEVAKIKAPVLMLIGDADFELRRIVNGRAFYALWERGVPVELQMYPMARRA
                     FDFRRDASPEEKIATRHARQRARDWLVRHMKLGADARCAA"
     gene            1278..1400
                     /locus_tag="AC16HB_00130"
     CDS             1278..1400
                     /locus_tag="AC16HB_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605223.1"
                     /translation="MLYRYFPKLCHPTRGQEINLRRLKGNRNVRVRTLPGKSRT"
     gene            1409..1606
                     /locus_tag="AC16HB_00135"
     CDS             1409..1606
                     /locus_tag="AC16HB_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605224.1"
                     /translation="MAYQTSVRHRRQVVEDPMYLDNLVETVGGEAVTSYDQKVTCCGG
                     ALAVSEPEKSQKPTRDIVESA"
     gene            1658..1840
                     /locus_tag="AC16HB_00140"
     CDS             1658..1840
                     /locus_tag="AC16HB_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605225.1"
                     /translation="MEIYQKDINKRYGTKCNMPVTYYSQLMTVAYGGSAKDAALNGQL
                     IRATKLEEIAARPAEN"
     gene            1851..2213
                     /locus_tag="AC16HB_00145"
     CDS             1851..2213
                     /locus_tag="AC16HB_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR5605226.1"
                     /translation="MPLTTDSIRGHEVEPHPNFRHAPAEWTPQLAQDVARKEKITLTE
                     DHWDVIRGLHEFYARHEDAPITLRDLHDALDEKFHHKGGVKYLYTLLPGGPIAQGCRL
                     AGLKPPGGAVDTSFGSVA"
     gene            complement(2477..3811)
                     /locus_tag="AC16HB_00150"
     CDS             complement(2477..3811)
                     /locus_tag="AC16HB_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR5605227.1"
                     /translation="METPRKPLYRSLYLQVIVAIVIGILLGQFFPETGTAMKPFGDGF
                     IKLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGGYALLYFEIVSSIALIVGLTIVNLV
                     QPGAGMNIDPASLDTRGIAAYAKPGQMQSTQEFLLAIIPNTLVDAFAKGEILQVLLIA
                     VLFGFCLHRFGGRGTLVFDMIEKTSHVLFGIVGVIMKLAPLGAFGAMAFTIGKYGAGS
                     LFSLGKLMGTFYLTCLVFILLVLGTISRMHGFGILKFIRYIKEELLIVLGTSSSESVL
                     PRMMAKMENLGVKKSTVGLVIPTGYSFNLDGTSIYLTMAAMFVAQATNTPMTLIQQLT
                     LLAVLLLTSKGAAGVTGSGFIVLAATLSAVGNVPVAGLALILGIDRFMSEARALTNLI
                     GNGVATVVVGKWCNELDTARMKRVLDREIELDAEEPEKIIDAVEQHMPVKAA"
     gene            complement(3878..4873)
                     /locus_tag="AC16HB_00155"
     CDS             complement(3878..4873)
                     /locus_tag="AC16HB_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5605228.1"
                     /translation="MRQRSDRALTRVVATGARESGRIGSRPAPAKWLSGILAVSAILL
                     AVPAAAQRAGPQIELWSSATLKRIDATAVVRLGYRENSPPFAFLNAKKQPVGYSLDIC
                     EVVVDEIVREIRKELRVEYRPVTPENRFELVASGAIDLECGSSTNTAERRKSVAFSPT
                     IFVTGTKLLVRRGSGIRTLRDLDGKTIVLTRGTVHETTVPRLAARQKLSIRFVFGADH
                     NESFQMLAAGKADAFANDDVQLYGMVAETRSASDFRVVGDFLTYADYALTLRKDDPEF
                     AEVVERAFHRLAGSREIVAIYERWFIRPLPSGVRLNLPMSPHLEELFQVQGLPKD"
     gene            complement(5368..6540)
                     /locus_tag="AC16HB_00160"
     CDS             complement(5368..6540)
                     /locus_tag="AC16HB_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605229.1"
                     /translation="MLLRLTLARACAWALLVAGWIGVGGLALQFAPSVSYAFALVALW
                     LLTLGAAAAIATRGSMHRSTRAMALLAGAAVTGFGVWSTVHGGGIPTLLLALTGWAAL
                     TALASGVVRSLRLAQTAIPTPPIAAATAGAVCAGLIIGDLGDTPALAMRLAVFVGAAA
                     ATLVLLQMRIDDRTGTPGCRAGLFDCSLPAWPAGAWRDTQQWPALLAGLAMLPMMAAL
                     PLMAEWCRAQAVAPQAMVLLHLASMFVPALLLRRSVARWSLRTLSTVCAVLLASGAAL
                     VTWAAAPLDLLGLAVTHGAAWGLAWSGQLWAPARRGRKGTSPVRAALGYAALTLSFGL
                     VVDHSGARGVAAVHAALGLAAALAWTLLALSNTWSRDAVPGGHARTAPPGSPGGQP"
     gene            complement(6558..7253)
                     /locus_tag="AC16HB_00165"
     CDS             complement(6558..7253)
                     /locus_tag="AC16HB_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5605230.1"
                     /translation="MGCIYKNSGLHSRMTVGAVVLAAGAGARLGGRPKALLELGGVPL
                     LMRQLIALSGAGVDEVVVVLGHHAEAVEAAVREFPITLAHNPSPDDGQASSVRVGLRA
                     LSPRLDAVIVALADQPLIATEDIVALISAFKKRGDAPMVVPRVQLDGEAPVPGNPVIF
                     DAALRDEWLSGSADLACRKWRDRNPERVRWFDTNNRRYVVDIDTAEDLKRFAASTGHR
                     LTWPAFLAHEPVE"
     gene            complement(7267..8403)
                     /locus_tag="AC16HB_00170"
     CDS             complement(7267..8403)
                     /locus_tag="AC16HB_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5605231.1"
                     /translation="MLSLDDGSPDARAVARLTGFPGFLRANGFAIGGGDAVPVLQTAQ
                     RVGVLDANLLRWSLQALLCGRCDEWQRFDELFDAWFLPANRWQRPEQRDVDSGALGAG
                     RTAAQTDDAANDDDVRPRRAASREEVLSSTDFRALTEREHALDIEALMRRLARQLRQI
                     RLRREARARRGRRLDLPATIRRSVASGGTPFHLAWKDRRRVRPRVVLLIDVSRSMALY
                     SFFYLRLARALSAELNDVYSFIFHTRVTAVSEALHDRDPWRAQERLHLIAQGWAGGTR
                     IGESLAQFNREHAPRLVHSRTAVVIMSDGYDTGDPAVLSEALAQLRRRARRVVWLNPL
                     SDRPGYAPICQGMQAALPHLDLLAPGADLASIRAVLPNLIEALG"
     gene            complement(8397..>9051)
                     /locus_tag="AC16HB_00175"
     CDS             complement(8397..>9051)
                     /locus_tag="AC16HB_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5605232.1"
                     /translation="MYEWNYQRQLLAIKLLEHDERGIAQKEQDIFSERYLLKRPLLEA
                     ITGEEPPVLLIDEIDRADAAFEAYLLELLSDFQLSIPELGTVHATHRPLVVITSNGTR
                     ELSDALRRRCLYQYIDYPDYEKELAIVRRKAPAVAAALAQQIVAFVQAVRRMELQKRP
                     GIAETLDWAAALLRLEISVIDVDGTERIVETLSALIKTRDDRAAFPREVVARLAAAC"
BASE COUNT         1596 a         3041 c         3046 g         1368 t
ORIGIN      
        1 atccacggca acaacctcgg cgacctgccg aaccttatgc ggctggcgta cgagtccgac
       61 gggaagctca cgtatcacgg cggcgaggcc acgctcgaac tcggcgggcg gcggctgttc
      121 atgatccatt acccgcatct ggcgcgcggg atggcgacca ccggcgattt cgacgcggtg
      181 ctgtgcgggc acagccacga agcgaagatc gagcaggtga ccaatgtgcg tggcggccgc
      241 acgccgctgg tgaatccggg cacggttgcc gggctcggcg cgccggcgac gatggtgctc
      301 ggcgacctcg ccacgctgtc gttcgaggtg caggcgctgg catgagagcg gccatcgtga
      361 ttctgctgct gctgtcgttc ggtgctgtcg cgcagccact cgaaacttac agtgtctcca
      421 ccggcaagta cgacgtgccg gttcacgttg ccaagccgac gcgcgccggg ccgttcgcac
      481 cagtgctgta cgtccacgcg cgccgcgggc tcgaggaaga agaccgcgcg cacatgcgcg
      541 aactcgcgca agcgggcttc ctcgtgctcg cgcccgactg gcaggccgcc aacatgatcg
      601 agcgctggcc cgaccgccac taccccgaga gcgaagacga cgtcgaagcg gggctgaacg
      661 cgctgctcgc tcggaaggac gtgtgcaagg gaccggtcgg cgtggtggga atttcgcgcg
      721 ggccgtactt cgcgatccgg ctggcggcga agcggccgca ggacatcgcg gcgatcgtct
      781 cgtactacgg ccacttccag aacccgaacg cgcccgaacc gcagcagctc tattcggttg
      841 cgcccgaggt ggcgaagatc aaggcgccgg tgctgatgct gatcggcgac gcggatttcg
      901 aactgcggcg catcgtcaac ggccgtgcgt tctacgcgct ctgggagcgc ggcgttccgg
      961 tcgaactgca gatgtacccg atggcgcgtc gcgccttcga cttccgccgc gacgcgtcgc
     1021 cggaggagaa gatcgccacc cggcatgcgc gccagcgcgc gcgcgactgg ctggtgcggc
     1081 acatgaagct cggcgccgat gcgcgctgtg cagcgtgaga agaggtgggg aaaccggatg
     1141 gctccagtcg cagccaaaga aacgatcctc gtggttggcg gcggcatcag cgactgaccg
     1201 cagcgctgga ggcgatcgac tgcggcaagc aggtcgtgct gaatcattag accgctacgc
     1261 ttggcggccg caccgcgatg ctgtaccgct atttcccgaa gctgtgccat ccgacacggg
     1321 ggcaggagat caacctgcgg cggctgaagg gcaaccgcaa cgtgcgtgtg cggacgctgc
     1381 cgggaaagtc gcgtacctga cgcacacgat ggcgtaccaa acgtccgtgc gccatcgtcg
     1441 gcaagtcgtg gaggacccga tgtacctcga caatctggtc gagaccgtcg gtggcgaggc
     1501 cgtcaccagc tacgaccaga aagtgacctg ctgcggcggc gcgcttgcgg tatcggagcc
     1561 ggagaagagc cagaaaccga cccgggacat cgtcgagagc gcgtaggatc acggcgcgga
     1621 cacgatcgtc acgccgtgcc cgctgtgcca ggccaacgtg gagatctacc agaaggacat
     1681 caacaagcgc tacggcacca agtgcaacat gcctgtgacg tactacagcc agctgatgac
     1741 ggtcgcctac ggcggcagcg cgaaggacgc cgcgctgaac ggccagttga tccgcgcgac
     1801 caagctggag gagatcgccg ccaggccggc ggagaattag ggaggcagtg atgccgctga
     1861 cgaccgattc gattcgcggt cacgaagtcg agccccatcc gaacttccgg cacgcgccgg
     1921 cggagtggac gccgcagttg gcgcaggacg tggcgcgcaa agaaaagatc acgctgaccg
     1981 aagatcattg ggacgtgatc cgcggcctgc atgagttcta cgcgcgccac gaggacgcac
     2041 cgatcacgct gcgcgacctg cacgacgcgc tcgatgagaa gttccaccac aagggcggcg
     2101 tgaagtacct gtacacactg ttgcccggcg gtccaatcgc gcagggttgc cggctggcgg
     2161 ggctcaagcc gcccggcggc gccgtcgaca cgagcttcgg cagcgtggcg tagcgccgcg
     2221 ccattgccgc cggcctgaat ccctcgacca cgaggggcgg ccggtgcttt gagccacagc
     2281 atccactccg cagtcaggcg gttccgtttt tgatcgatgg tggctcggcc gccgcggtcc
     2341 cggagggcgc ggtcgacgtc gacggaacga tgcgcccggc cgaggttcca tgtcgacggt
     2401 tcggacccga agctgcgcgg cgaccacgcg cccacgtcag ccactaccgc agcgaccgcg
     2461 atcaaggagc ggcggatcag gcggccttga ccggcatgtg ttgctcgacg gcgtcgatga
     2521 tcttctcggg ttcctcggca tcgagctcga tttcccgatc gagcactcgt ttcatccgcg
     2581 cggtgtcgag ttcattgcac cacttgccca cgaccacggt cgcgacgccg ttgccgatca
     2641 gattggtcag cgcccgggct tccgacatga agcggtcgat gccgaggatc aatgcgagcc
     2701 cagcgaccgg cacgttgccg accgccgaca aggtggcggc gagcacgatg aagccgctgc
     2761 cggtgactcc ggccgcgccc tttgaagtaa gcagcagcac ggcgagcagc gtcagctgct
     2821 gaatcagcgt catcggcgtg ttggtggcct gcgcgacgaa catggccgcc atcgtgaggt
     2881 agatcgacgt gccgtcgagg ttgaacgagt atccggtggg aatgaccagc ccgaccgtcg
     2941 acttcttcac gccgaggttc tccatcttgg ccatcatgcg cggcagcacc gactccgacg
     3001 acgacgtgcc gagcacgatc agaagctcct ctttgatgta gcggatgaac ttcaggattc
     3061 caaacccgtg catgcgcgag atcgtgccca gcacgagcaa tatgaacacc agacaggtga
     3121 ggtagaaggt gcccatcagc ttgccaagcg aaaacagcga cccggcgccg tacttgccga
     3181 tcgtgaaggc catcgcaccg aacgcgccga gcggcgcgag tttcatgatc acgcccacga
     3241 tcccgaacag cacgtgcgag gtcttctcga tcatgtcgaa caccagcgtg ccacggccgc
     3301 cgaaccggtg cagacagaac ccgaacaaca ccgcgatcag cagcacctgc aggatctcgc
     3361 ccttggcaaa cgcgtcgacc agcgtgttgg gaatgatcgc gagcaggaac tcctgcgtgc
     3421 tctgcatctg gcccggcttc gcataggcgg cgatgccccg ggtgtcgagg gaggcggggt
     3481 cgatgttcat gccggcgccc ggctgcacga ggttgacgat ggtcagtccg acgatcagcg
     3541 cgatgctgct gacgatctcg aagtacagca gcgcgtagcc gccggtcttg ccgaccttct
     3601 tcatgtcctc catgccggca atgccgacga cgacggtaca gaagatgatc ggcgcgatga
     3661 tcatcttgat gagcttgatg aacccgtcgc cgaacggttt catcgccgtg ccggtctcgg
     3721 gaaagaactg gccgagcaag atgccgatga cgatggcgac gatgacctga aggtacagcg
     3781 acctgtaaag cggcttgcgc ggcgtttcca tgcgcgttcc tcctgtggtc ggtgcttgcc
     3841 ttgttctcga cgccgggggc ggtgctcgtg atccgaatca gtccttcggc aggccctgga
     3901 cctggaagag ttcctcgagg tgcgggctca tgggcagatt cagacgcacg ccggacggca
     3961 ggggcctgat gaaccagcgc tcatagatcg cgacgatctc gcgactgccc gccaatcgat
     4021 gaaacgcgcg ctcgacgact tcagcgaact ccggatcgtc cttgcgcagc gtcagcgcgt
     4081 agtcggcgta ggtgaggaaa tcgccgacca cgcggaaatc gctggcggaa cgggtctcgg
     4141 ccaccatgcc atagagctgc acgtcgtcgt tcgcaaatgc gtcggccttg cctgccgcga
     4201 gcatctgaaa cgactcgttg tggtcggcgc cgaagacgaa cctgatcgag agtttctgcc
     4261 gcgcggcgag ccgcgggaca gtcgtctcgt gcacggtgcc gcgcgtcagc acgatcgtct
     4321 tgccgtcgag gtcgcgcagc gtacgaatgc cgctgccgcg ccggacaagc agcttggtcc
     4381 cggtcacgaa gatcgtgggc gagaaggcca cgctcttgcg ccgctcggcg gtgttggtcg
     4441 atgagccgca ctcgaggtcg atcgcgccgg acgcgacgag ttcgaatcga ttctccggcg
     4501 tcaccggccg gtactcgacg cggagctcct tgcggatctc gcgcacgatc tcgtcgacga
     4561 cgacttcgca aatgtcgagc gagtagccga ccggctgctt cttcgcattc agaaaggcga
     4621 acggcggcga attctcgcgg tagcccaagc gcacgaccgc cgtcgcgtcg atcctcttga
     4681 gcgtggcaga agaccagagt tcgatctgtg ggcctgcgcg ctgcgcggcc gccggcaccg
     4741 ccagcaggat cgcagaaacg gcgaggatgc cggacagcca tttcgccgga gcaggacgcg
     4801 atccgatgcg accactctct cgagcgccgg tggcgacgac ccgcgtgagg gcgcgatcgc
     4861 tccgctgtct catggctcag ctctccctta ccagcgccgt atcgctcgct gcatcggctt
     4921 gctccaccgc acgccgcagg ttgcgcattc gcctgcggtc ggatcggcag ttcgcagcga
     4981 caggcgtcga tcatattgcc tgctgtccgc ggcaggtgcc agcgcgcgtc gtgaacgggt
     5041 tagtccccgc atcggttgct gtagtgcgta ggtgcgaaca gctgcgagag cgtgatcgcc
     5101 agaaagccgc gcatgccgcg ggcccaatcg aacgcgacgc ggatctgtgt ggggcacggc
     5161 cggcggatgc ggccagcgca acgttcgagc gcatggcgaa gagcgtcccg cacgcaatgc
     5221 caacgcgcgc gaactgtccg tgactcgcat cgacgttgcg gaacgcgaag gtcgaaccgc
     5281 gcgcatctag cgggcgccag cgccgaccga gtgagcgaaa ggcctcgccg ctctatctca
     5341 tgggcagcga cggcgcgccg atctggatca cggttggccg ccgggagaac cgggaggcgc
     5401 ggttcgcgcg tgcccgccgg gaaccgcgtc gcgtgaccag gtgttcgaca acgccaggag
     5461 tgtccaggcc aacgctgcag cgaggcccaa tgccgcgtgc acggcggcca cgccgcgtgc
     5521 accgctgtga tcgaccacca gcccgaacga gagcgtcagc gccgcgtacc caagggcggc
     5581 acgcaccggc gaggtgccct tccggccgcg gcgggccggc gcccacaact ggccgctcca
     5641 cgccaggccc caggccgcac catgcgtgac ggccagaccc agcagatcca gcggcgctgc
     5701 cgcccacgtc accagcgccg cccccgaggc gagcaggacg gcgcacaccg tcgataaggt
     5761 gcgcagcgac cagcgcgcaa ccgaccgcct cagcagcagt gctgggacga acatcgacgc
     5821 cagatgaagc agcaccatcg cttgcggggc aacggcctgc gcgcggcacc actcggccat
     5881 cagcggcaac gcagccatca tcgggagcat ggccagcccg gcgagcagcg cgggccactg
     5941 ttgcgtgtcg cgccacgctc ccgcgggcca ggccggcagc gaacagtcga acaggcctgc
     6001 gcggcatccc ggcgtgcccg tgcgatcgtc gatccgcatc tgcagcagca cgagcgttgc
     6061 agcggccgcg ccgacgaata cggcaaggcg catggcaagc gccggcgtgt cgccgaggtc
     6121 tccgataatc aggccggcgc acacggctcc cgccgttgcg gctgcgatcg ggggcgtagg
     6181 tatggcggtc tgagccaggc gcaggctgcg caccacgccc gacgccagtg ccgtcaacgc
     6241 ggcccagccg gtcagcgcaa gcagcagggt ggggatgccg ccgccatgca ccgtcgacca
     6301 cacgccgaaa ccggtgaccg ctgcaccggc caacaaggcc atcgcgcgcg tcgatcggtg
     6361 catgctgccg cgcgtggcga tcgcggccgc ggcgcccagg gtcagcagcc agagcgcgac
     6421 cagcgcaaac gcatagctca ccgagggagc gaactgcaac gcgagaccgc cgacgccgat
     6481 ccaccctgcg acgagcagcg cccaggcgca cgcccgcgcc agtgtcaggc gcagcagcat
     6541 cgaggcattt ccagtcttca ttcgacaggt tcgtgtgcga gaaacgccgg ccacgtcagc
     6601 cgatggcccg tgctcgcggc gaagcgcttc aggtcttcgg ctgtgtcgat gtcgaccacg
     6661 tagcgccgat tgttggtgtc gaaccagcgc acgcgctccg ggttcctgtc gcgccacttg
     6721 cggcaagcaa gatcggcgct gcccgagagc cattcatcgc gcagcgcagc gtcgaagatc
     6781 accggattcc cgggtacggg cgcctcaccg tccaattgca cgcgcggcac gaccatcggc
     6841 gcatcgccgc gcttcttgaa tgcgctgatc agcgccacga tgtcctcggt agcgatcagc
     6901 ggttgatcgg caagcgcgac gatcactgcg tcgagacggg gcgacagcgc acgcagcccg
     6961 actcgcacgg aggacgcctg gccgtcatcg ggcgacgggt tgtgtgccag cgtgatcggg
     7021 aactcgcgca ccgcagcctc gaccgcttct gcgtggtgac cgagcacaac gacgacctcg
     7081 tccacccctg cgccggacaa tgcgatcagc tggcgcatca gcagcggaac gccgcccagt
     7141 tcgagcaatg ccttgggccg cccaccgagc cgcgcgccgg cgccggccgc gagcaccacg
     7201 gcgcccaccg tcatccggct gtgcaggccg ctgttcttgt agatgcagcc catgctccgt
     7261 ctccttctag ccgagggctt cgatcaggtt gggcagaacc gcgcggatgc tcgccaggtc
     7321 ggcgcccggc gccagcaggt ccagatgcgg cagcgccgcc tgcatgccct ggcagatcgg
     7381 cgcgtagccg gggcgatcgc tcaacgggtt cagccacacc acccgccgtg cacggcgacg
     7441 caactgcgcc aatgcctccg acagcaccgc ggggtcgccg gtgtcgtaac catcgctcat
     7501 gatgacgacg gcagtgcgcg agtgtacgag gcgcggcgcg tgctcgcgat tgaactgcgc
     7561 cagactttcg ccgatgcgcg tgccgccggc ccagccctgc gcgatcagat gcagccgctc
     7621 ctgcgcgcgc cacgggtccc gatcgtgcag ggcttcggac actgcggtca cacgggtgtg
     7681 aaagatgaag ctgtagacgt cgttcagctc ggcgctgagc gcgcgcgcaa ggcgcaggta
     7741 gaagaagctg tacagcgcca tcgaacggct gacatcgatc aacagcacca cgcggggccg
     7801 cacacggcgg cggtccttcc aggccagatg gaaaggcgtt ccgccgctcg ccacgctgcg
     7861 ccggatcgtt gccggaagat cgaggcgacg gccacgccgg gcgcgcgcct cgcggcgcag
     7921 acggatctgc ctcagttgtc gggcgagacg ccgcatcaag gcctcgatgt cgagcgcatg
     7981 ctcgcgctcg gtcagggcgc ggaaatcggt cgagctcagc acctcttcgc ggctcgccgc
     8041 gcgccgggga cgcacgtcgt cgtcgttcgc cgcgtcgtca gtctgcgcgg cggtccgtcc
     8101 agcgccaagc gcgccgctgt cgacgtcacg ctgctcgggc cgctgccagc ggttggcggg
     8161 caggaaccag gcatcgaaca gttcatcgaa gcgttgccac tcgtcgcatc ggccgcacag
     8221 caacgcctgc aggctccagc gcagcaggtt cgcatcgagc acgcccacgc gctgcgccgt
     8281 ctgcaggacc ggcacggcgt cgccgccgcc gatcgcgaaa ccgttggcgc gcaggaagcc
     8341 ggggaatccc gtcaggcgcg cgacagcccg cgcgtccggc gatccgtcgt ccaggctcag
     8401 catgcggcgg ccagccgtgc aacgacctcg cgcggaaacg cggcgcggtc gtcacgggtc
     8461 ttgatcaggg cgctcagcgt ctcgacgatg cgctcggtgc cgtcgacgtc gatcaccgaa
     8521 atttccagcc gcagcagtgc cgcggcccag tccagcgtct cggcgattcc gggtctcttc
     8581 tgcagctcca tgcggcgcac ggcctgcacg aaggcgacga tctgctgcgc gagcgcggcg
     8641 gccacggccg gcgccttcct gcgcacgatg gcgagttcct tttcgtagtc cgggtagtcg
     8701 atgtactggt acagacagcg gcggcgcaac gcatccgaaa gctcgcgcgt gccgttggac
     8761 gtgatcacga ccagcggtcg gtgggtggca tgcacggtgc cgagctccgg gatcgagagc
     8821 tggaagtccg acagcagttc gagcaggtac gcctcgaacg ccgcatccgc gcgatcgatt
     8881 tcgtcgatca gcagcaccgg tggctcttcg cccgtgatgg cttcgagcag cggccgcttc
     8941 agcaggtagc gctcggagaa gatgtcctgc tccttctgtg cgatgccgcg ctcgtcgtgc
     9001 tcgagcagct tgatcgccag cagctgacgc tggtagttcc attcgtacat c
//
LOCUS       DBNHBY010000005         3321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1353683, whole genome shotgun sequence.
ACCESSION   DBNHBY010000005 DBNHBY010000000
VERSION     DBNHBY010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1353683"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC16HB_00180"
     CDS             complement(<1..1190)
                     /locus_tag="AC16HB_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5605233.1"
                     /translation="MNHTERTLRDLLARRILILDGAMGTLIQRYRLTEADFRGARFAD
                     HPVDVRGNNELLLLTKPELIREIHDAYLAAGADIISTDTFGGTSVAQHDYRMGHLAYE
                     MNVAAARIAKAAAAQFTTAEKPRFVAGSLGPTPKTASISPDVNDPGARNVTFDELAAS
                     YGEQTRGLLDGGADILLVETIFDTLNAKAAIFAIEAEFERRGQRAPVIISGTVTDASG
                     RILSGQTVEAFWHSVRHARPLAAGLNCALGAALMRPYIEEFARIADVFISVYPNAGLP
                     NPMSETGFDETPAVTSALLREFAQAGFVNVAGGCCGTTPEHVRAIADAIEGIAPRVVP
                     APHRALRLSGLEPLTVTDESLFVNVGERTNVTGSKAFARLILAEKYEEAVAVARQQVE
                     NGAQV"
     gene            1351..1743
                     /locus_tag="AC16HB_00185"
     CDS             1351..1743
                     /locus_tag="AC16HB_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5605234.1"
                     /translation="MKQAKPREAYEILQNNPNALLIDCRTEFEFYYVGHPVGAINVEW
                     QTAPDFDHNPRFVEGVMKEAAGDRNRPVLLICRSGRRTLEAATALEAAGFTDVTNVLE
                     GFEGELDRTHHRSTINGWRVSGLPWQQL"
     gene            1786..3066
                     /locus_tag="AC16HB_00190"
     CDS             1786..3066
                     /locus_tag="AC16HB_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605235.1"
                     /translation="MQHPPARLSYLVWGLAAAFYLFGFFQRVTPASLAADLMRDFHLS
                     AAALGNLSAFYYYAYAAIQIPTGILVDRWGPSRLFVTGAALSGVGALLFALGDSVLVA
                     GCGRALIGAAHGMAWVSMLKLVTHWFPMQRFGTLSGLSLAVGTLGAILAGPPLRALAD
                     AFGWRSVIAVSGGAVLLLAMAIRIFMRDDPLDRNYASHAPASARARADARAPITDGLR
                     EIFRRRNVWLLPIVNSGTCGAFLAFTGLWGVPFFVQQHALTVKQASTVTSAMLVLFAL
                     GAPAFGHLSDHWQLRKRPFALGTALLLLGFGTLALFPAAPLPLLIPLLLAAGFGAAAM
                     ALSFGYVKESVPAQYQGLVTGVANAGVMLGTLIQMPVIGIILDAHWKGTIVDGVRQYD
                     LQAFQYALLFLFAWLAAAFLLLLVTRETHARQMP"
     gene            complement(3090..>3321)
                     /locus_tag="AC16HB_00195"
     CDS             complement(3090..>3321)
                     /locus_tag="AC16HB_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTH_I0359 family protein"
                     /protein_id="HIR5605236.1"
                     /translation="FCVVEFPSLGDEGTQSSGGFEIMDKQLRREIFLGGKDAEEFRAS
                     VAKLISSEPTADDIDDFLGGYSGLMTTPVTLH"
BASE COUNT          475 a         1131 c         1137 g          578 t
ORIGIN      
        1 acctgcgcgc cgttctcgac ctgctggcgc gcgacggcga ccgcttcctc gtacttctcc
       61 gccaggatca gccgcgcgaa tgccttcgag ccggtgacgt tggtgcgctc gccgacattg
      121 acgaacagcg actcgtcggt gacggtgagc ggctccagtc cggacagccg cagcgcgcga
      181 tgcggcgccg gcacgacgcg gggcgcgatg ccctcgatcg cgtcggcgat cgcgcgaacg
      241 tgctccggcg tggtgccgca gcagccgccg gcgacgttga cgaagcccgc ctgcgcgaat
      301 tcgcgcagca gcgccgacgt gacagccggc gtttcgtcga acccggtttc cgacatcggg
      361 ttcggcaggc cggcgttcgg atacacgctg atgaagacgt cggcgatgcg cgcgaattcc
      421 tcgatgtacg gccgcatcag cgccgcgccg agcgcgcagt tcaggcccgc cgcgagcggc
      481 cgcgcatgcc gcaccgaatg ccagaacgcc tcgaccgtct gacccgacag gatgcggccc
      541 gatgcgtcgg tcaccgtgcc ggaaatgatc acgggtgcgc gttgtccgcg ccgttcgaat
      601 tcggcctcga tggcgaagat cgccgccttg gcgttcagcg tgtcgaagat cgtctccacg
      661 agcaggatgt cggcgccgcc gtcgagcagt ccgcgcgtct gctcgccgta ggacgccgca
      721 agctcgtcga atgtcacgtt gcgcgcaccg gggtcgttga cgtcgggcga gatcgacgcg
      781 gtcttcggcg tcggccccag cgaacccgcc acgaagcgcg gtttctccgc cgtcgtgaac
      841 tgcgccgcag ccgccttcgc gatacgcgcg gcggcgacat tcatctcgta ggccagatgt
      901 cccatccggt agtcgtgctg cgcgaccgac gtgccgccga aggtgtcggt cgagatgatg
      961 tcggcgccgg ccgcgagata tgcgtcgtgg atctcgcgga tcagctcggg cttcgtcagc
     1021 agcagcagtt cgttgttgcc gcgcacatcg accgggtggt cggcaaagcg cgcgccgcgg
     1081 aagtcggctt ccgtcagccg gtagcgctgg atcagcgtgc ccatggcgcc gtcgaggatc
     1141 aggatgcggc gcgcgagcag gtcgcgcagc gtgcgctcgg tgtggttcat cgacttgacg
     1201 cgcgcggcgg cgcggagccc ggggcccggt attctagggg agcgcgcggc tgtgacatac
     1261 ccgttggcta tgaggatatg cacggctgcg ggtagcacac gcgccgcccg tggcgataat
     1321 gacgactcct cttgcgctga tccgacgacc gtgaaacaag ccaagccgcg cgaggcctac
     1381 gagatcctgc agaacaaccc gaacgcgctg ctgatcgatt gccgcacgga gttcgagttc
     1441 tattacgtcg gccatccggt cggcgcgatc aacgtcgagt ggcagaccgc gcccgacttc
     1501 gatcacaacc cgcgcttcgt cgagggcgtg atgaaggaag ccgccggcga ccgcaaccgt
     1561 ccggtcctgc tgatctgccg ctccgggcgg cggacactgg aagctgccac cgcgctcgaa
     1621 gcggcgggct tcaccgacgt gaccaacgtg ctcgagggat tcgagggcga actcgaccgc
     1681 acccatcacc gcagcacgat caacggctgg cgcgtgtccg gcctgccctg gcagcaactg
     1741 tagcgaacgc ttttgcgtgc gaacgcgtgc catacactgg cgcgcatgca gcatccgcct
     1801 gcgcgactga gctatctggt ctgggggctg gcggccgcgt tctacctgtt cggcttcttc
     1861 cagcgcgtca cgccggcgtc gctcgcggcg gatctgatgc gcgatttcca cctgtccgcg
     1921 gctgcgctcg gcaacctgtc ggccttctac tattacgcgt acgcggcgat ccagatcccg
     1981 accgggatcc tggtcgatcg ttggggaccg tcgcggctgt tcgtcactgg cgccgcgctg
     2041 tcgggcgtcg gggcgctgct gttcgcgctc ggcgacagcg tgctggtcgc gggctgcggc
     2101 cgcgcgttga tcggcgccgc gcacgggatg gcgtgggtat cgatgctgaa gctcgtcacg
     2161 cactggtttc cgatgcagcg cttcggcact ctgtcgggcc tctcactggc ggtcggcaca
     2221 ctcggcgcga tcctcgccgg cccgccgctg cgcgcgctgg cggacgcgtt cggctggcgc
     2281 agcgtgatcg cggtgtcggg cggcgcggta ctgctgctcg caatggcgat ccggatcttc
     2341 atgcgcgacg atccgctcga ccgcaactac gccagccatg cgccggcgag cgcgcgcgcg
     2401 cgtgccgatg cgcgcgcacc catcacggac ggcctgcgcg agatctttcg ccgccgcaac
     2461 gtgtggctgc tgccgatcgt caattcgggc acctgcggcg cgttcctcgc gttcaccggg
     2521 ctgtggggcg tgccgttttt cgtgcagcag catgcgctga ccgtcaagca ggcatcgacc
     2581 gtaacgtccg cgatgctcgt gctgttcgcg ctcggtgcac cggcgttcgg ccacctgtcg
     2641 gaccactggc aactgcgcaa gcggccgttc gcgctcggca ccgccctgct gctgctcggc
     2701 ttcggcacgc tggcactgtt tcccgccgct ccgctgccgc tgctgatacc gctgctgctg
     2761 gcggcggggt tcggcgccgc ggcgatggcg ctgtcgttcg gttacgtgaa ggaatcggtg
     2821 ccggcacagt accagggcct ggtcaccggg gtcgccaacg ccggcgtgat gctcggcacg
     2881 ctgatccaga tgccagtgat cgggatcatc ctcgacgcgc actggaaggg cacgatcgtc
     2941 gacggcgtgc gccagtacga cctgcaggcg ttccagtacg cgctgctgtt cctgttcgcg
     3001 tggctggcgg cggccttctt actgctgcta gtcacccgcg agacgcacgc ccgccagatg
     3061 ccgtgacgga cgtcgcgcga tgcggcgatt cagtgcagcg tgaccggcgt cgtcatcagg
     3121 cccgagtaac cgcccaggaa atcgtcgata tcgtcggcgg tcggctcgct ggagatcagc
     3181 ttggccacgc tggcacggaa ctcctcggcg tccttgccgc caagaaagat ctcgcgccgc
     3241 agctgcttgt ccatgatttc gaagccgcca gacgactgcg ttccttcgtc gccgagcgag
     3301 gggaattcga ccacgcagaa c
//
LOCUS       DBNHBY010000006        11622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1827394, whole genome shotgun sequence.
ACCESSION   DBNHBY010000006 DBNHBY010000000
VERSION     DBNHBY010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1827394"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="AC16HB_00200"
     CDS             complement(<1..415)
                     /locus_tag="AC16HB_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR5605237.1"
                     /translation="MNTRRALLCAAGGALLAPRARAASIDFPAVTPRPLAFPRDHGAH
                     PDYRTEWWYLTGWLDAKPEQIGFQVTFFRTRTTIDPANPSRFAARQLLIAHAAIAQAS
                     RGHLLHDQRIARAGFGIARAAQTDTDVVLDRWTLQR"
     gene            complement(412..2958)
                     /locus_tag="AC16HB_00205"
     CDS             complement(412..2958)
                     /locus_tag="AC16HB_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5605238.1"
                     /translation="MRWLLAHVIGGYARAHPLRAAVQVVAIAVGVALGFAVHLINSSA
                     LAEFSSALRQVSGQADASIVGAPGASGGFDERLVERVAADPRVELASPMLTLDVALAA
                     PERLRGKTLTIVGVDVFRAAQLAPQWIGAPVQDDRLALLGDGLYLSPAAFEALRVAPG
                     DSIGVLIGGRVVTLRIAGRLPAARAGAVVAAMDIGFAQWKLAQLGRLTHIDLKLAPGV
                     DLATLAKDWQLPAGVSLQPADAAALRVSNLSRAYRVNLNVLALVALFTGAFLVYSLQS
                     QSVLARRAQLAFLRVLGTTRGEVEALLVLEAAALGIVGALLGIALGTLIASVALELLG
                     GDLGSGYFSGARPPLAFDSVNAAGFFALGVAAAIIGGWLPARDAARDTPAYALKSGSG
                     LDADARPARAWLGLALLVFAGALLLAPPIAGIPVFAYLSIAVLLTAAIFIKPLIAPHV
                     FVPLARLASRRTGVGAARWLATTRLAATPRFASIGAAGIVASFALMVAMATMVASFRA
                     SVDDWLTRVLPADIYVRAGPLASGGATTVFSAADIERLRGAPLAARAEFTRTVRIVLD
                     PARAPVSLVARPIDRADPAQRLPLTGRALAWHDGMPPPAWVTEPIVDLYGARVGGTIG
                     VPLGGTVHRFVVAGVWRDYARQFGAIAIDAADYTRLTGDTAFTEAALWLKPGVRAAAV
                     IAELQPTLDAKTAEFAEPGEIRAVSLRIFDRSFAVTYVLEIAAIVIGLTGIAATFSAQ
                     AIARTREFGMLRHVGVTRAQILRLLATEGLLVTLLAIGIGLAAGLAVAWVLVAIVNPQ
                     SFHWTMDFRLPVAQIAGLVAALLIAAALTAVIAGRRAVASAAVLSVREDW"
     gene            3309..6017
                     /gene="mgtA"
                     /locus_tag="AC16HB_00210"
     CDS             3309..6017
                     /gene="mgtA"
                     /locus_tag="AC16HB_00210"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HIR5605239.1"
                     /translation="MVTSHSVVDFFRSVFGLRSVVARPAAANPSFAHLNEIAQLSTEA
                     ALARLDSSDEGLLERESLERLERYGPNEVAHEQKKHPLRRLLELFASPLPLLLLVLAL
                     VADLTGEVRGAIVITLMVMLSVLLSFVQEFRSSRAAEKLRAMVHTTVTVLRKDVRKGV
                     PDEVNRYFQVRLQYQEPKKVEIPLARLVPGDVVTLSAGDMIPADVRILSAKDLFVSQA
                     ALTGESLPVEKFAAPSNAGTTPLELANIGFMGTNVISGTALAVVAATGRSTYFGSIAA
                     TVTGTRQLTSFDLGVNRFIWLMIRFMAVMVPLVFVINGLSKGNWLEAFLFAVAVAVGL
                     APEMLPMIVTINLGKGALAMARKKVIVKRLNSIQNFGAMDVLCTDKTGTLTQDKVILE
                     RHVDIFGKDSDHVLQYAYLNSYHQSGLKNLLDVAILKHIEVHEALEVNKHYGKTDEIP
                     FDFQRRRMSVVVEKDHATHILICKGAVEEIFSVCTAVERDGVRVPIEPDHLDDLQEVI
                     RDLNDDGFRVIAVAFKEVPTRVQPYAVADECDLTLVGYVAFLDPPKDTAAAALKALAQ
                     HGVRVKVLTGDNDVVTRKVCRDVGLDTQHVLLGADLEKLKDDELDARVDEATVFAKLT
                     PQQKARVIGALHRRGHVVGFLGDGINDGPALKAADVGISVDTAVDIAKESADIILLEK
                     SLLVLEAGVLEGRAVFGNITKYIKMGASSNFGNMFSVLGASAFLPFLPMAPVQILLNN
                     LMYDFSQTAVATDNVDREYVEKPRKWDIGNIGRFMLFIGPISSIFDYATFLTMLYLFD
                     AWTNAPLFQTAWFVESLLSQTLIVHVLRTGGLPFVHTRPSLPLAMTTLAICVLGLWLP
                     TSPFAEALGFTPLPGGCWPALLVIVAAYLGLTQLVKTWLIRRFGLD"
     gene            complement(6031..6690)
                     /locus_tag="AC16HB_00215"
     CDS             complement(6031..6690)
                     /locus_tag="AC16HB_00215"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605240.1"
                     /translation="MLVLSNVRKRFGARILLDGIDLRAGSGELIAIIGESGSGKSTLL
                     NLIAGLDLPDAGRIDIDGQSLSELSDDARTRLRRHRIGFVFQAFHILTHMTVAQNVEL
                     PLVLLGVDADQREARVRDLISAVGLAGRERSPPRELSGGELQRVALARALVHRPALVL
                     ADEPTGNLDPDTADSVLALIAATIRGRGAAGVLVTHSLHAAQTADRVLRLEHGRLVAA
                     R"
     gene            6732..7952
                     /locus_tag="AC16HB_00220"
     CDS             6732..7952
                     /locus_tag="AC16HB_00220"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5605241.1"
                     /translation="MPATDPVTIDDIRAAAERLRGQVENTPCLHSRTLSQITGADVYL
                     KFENLQFTASFKERGALNRLAQLDGEQKQRGVIAVSAGNHAQGVAYHAQRMGVPAVIV
                     MPRFTPMVKVERTRGFGAEIILRGDTFDDAREEALTIAAARRLTLVHPYDDGAVIAGQ
                     GTIGLEMLVAQPQLDCLCMAIGGGGLISGVAIAARALHPAIEVIGVQTEQFPSMYALF
                     KGIEMPCANATLAEGIAVKSPGLITRGIVRRMVNDIVLVSEGDIEHAIVLLLEIEKNV
                     VEGAGAAPLAALIRHPQRFAGKRVGLVLSGGNIDPMVLGDIIERGMVRAGRLARIRVA
                     TRDVPGELARAANIVGATGANIEEVEHQRAFTTLPAQNAQLEMVLQTRGPEHIEEILR
                     ALRDAGLPAELHTY"
     gene            8058..8591
                     /locus_tag="AC16HB_00225"
     CDS             8058..8591
                     /locus_tag="AC16HB_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605242.1"
                     /translation="MAARTSNRKTGAAVARKGMARSRTGWEVKLRPELQPRVVRDPRQ
                     GDQLLIPTPLLVAEEVGRVARGKVLTLSELRTSLAKRFKADRTCPMTTGIFAAIVAGA
                     VREDIARRRKPRWPIWRLVRDDGSLNPNWPLSGRYRAALLRDEGMTVTRAGTTWRALV
                     VVADESRKNSRVAVRHM"
     gene            complement(8697..8822)
                     /locus_tag="AC16HB_00230"
     CDS             complement(8697..8822)
                     /locus_tag="AC16HB_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605243.1"
                     /translation="MNAARVPAEQVETEEQESSLAELTVTELAYVGGGMANFSFV"
     gene            complement(8852..8980)
                     /locus_tag="AC16HB_00235"
     CDS             complement(8852..8980)
                     /locus_tag="AC16HB_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605244.1"
                     /translation="MNDTLIQSAPETAETKDIALVTELQVAELALVGGGMANFSFV"
     gene            complement(9012..9146)
                     /locus_tag="AC16HB_00240"
     CDS             complement(9012..9146)
                     /locus_tag="AC16HB_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605245.1"
                     /translation="MNATLIQSSEPVVQQEVDAQEELNELRAVELSLVGGGMANFSFV
                     "
     gene            9416..11173
                     /locus_tag="AC16HB_00245"
     CDS             9416..11173
                     /locus_tag="AC16HB_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5605246.1"
                     /translation="MLFSNAHAVLRQLSPQIVTRSRVDALLSVAQFLYVSGQPFAGIE
                     PAAESVSYARRLEQRGELRRALTFHGILLADTGNLPGAIECFAEAIDVAHAIGDVVGE
                     GSAWNNLGAALIYAAQYAEAIQCLERAIEIAQNEPRLATVRLQALSNVALACLHLEDY
                     AKGLRTAKIAVESAAEPKSPTELLSRVLSESYYTRLLLEVDSLERAKERCELAKRFAK
                     ESRSERAELIAALSEGLYEVHAGMIDVGLSRLTNALERARILKSALRDALAAMVKAHE
                     VAGKPDLALVYLRELMLHIKKAQQDNALRHHQLHIEKLERQQQAAASAEVVMERREQQ
                     LRGQFAEQVAQQELMRSRIEMLERLAVTAELRDDSTGEHSYRVGKLAALLAAEFGCDE
                     QTCFMIDLAARLHDIGKIGIPDGILLKPDRLTDAERQIMQTHTTVGAELLAQSNIPHM
                     QMAEEIARFHHEWWDGSGYPFGIGGSAIPIAPRITALADVFDALTHKRPYKEPWPVAR
                     ALEEIAYLKGHQFDPQLTDLFLALIPRLQREQGDLDEFLGRSARQSPFIQAREKIAAT
                     LRQASGRGMGARQRFDAQR"
     gene            complement(11164..>11622)
                     /locus_tag="AC16HB_00250"
     CDS             complement(11164..>11622)
                     /locus_tag="AC16HB_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5605247.1"
                     /translation="ARDWDHAAYRGRIGVVMQDDQLFVGTIEDNISFFDPQHDAERVR
                     ECARLARIHEDIEAMPMQYNTIVGSMGIALSGGQKQRLILARALYRRPQALFLDEAFD
                     QLDLAREREITDGLKHLGMGLVIVSHRPETVRAVDRLVALGPRAGSSPYL"
BASE COUNT         1960 a         3859 c         4014 g         1789 t
ORIGIN      
        1 gccgctggag cgtccagcgg tcgaggacga cgtcggtgtc ggtctgcgcc gcgcgcgcga
       61 tgccgaatcc ggcacgtgcg atgcgctggt cgtgcagcag gtggccgcgc gacgcttgcg
      121 cgatcgccgc gtgcgcgatc agcaactgcc gcgcggcgaa ccggctcggg ttcgcgggat
      181 cgatcgtcgt ccgcgtgcgg aagaaggtca cctggaagcc gatctgctcc ggtttcgcat
      241 cgagccagcc agtcaggtac caccactcgg tgcggtaatc gggatgggcg ccgtgatcgc
      301 gcgggaaggc gagcgggcgc ggcgtgaccg cgggaaagtc gatgctggcg gcccgcgcgc
      361 gcggcgcaag cagcgcaccg cctgccgcgc acagcagcgc gcggcgcgtg ttcaccagtc
      421 ctcccgcacc gacagcaccg ccgcggacgc gaccgcgcgc cggccggcga tcacggccgt
      481 cagtgcggca gcgatgagca aagcggcgac gaggccggca atctgcgcga ccgggaggcg
      541 gaaatccatc gtccagtgga acgactgcgg gttgacgatc gccaccagca cccacgcgac
      601 ggcgagcccc gccgccagtc cgatgccgat cgccagcagc gtgaccagca ggccttccgt
      661 ggcgagcagc cgcaggatct gcgcacgcgt cacgccgacg tggcgcagca tgccgaactc
      721 gcgcgtgcgc gcgatggcct gcgccgagaa cgtcgccgcg atgccggtga gcccgatcac
      781 gatcgccgcg atctcgagca cgtaggtgac cgcaaagctg cgatcgaaaa tgcgcaggct
      841 gactgcgcga atctcgcccg gctcggcgaa ctcggccgtc ttcgcgtcga gcgtcggctg
      901 cagttcggcg atcacggcag ccgcccggac gccgggcttc agccacagtg ccgcttccgt
      961 gaacgcggtg tcgccggtca ggcgcgtgta gtcggccgcg tcgatcgcga tggcgccgaa
     1021 ctggcgcgca tagtcgcgcc agacgccggc gacgacgaag cggtggacgg ttccgccgag
     1081 cggcacgccg atcgtgccgc ccacgcgcgc gccgtagaga tccacgatgg gctcggtcac
     1141 ccacgccggc ggcggcatgc cgtcgtgcca agcgagtgcg cggccggtca gcggcagccg
     1201 ctgcgcgggg tcggcgcgat cgatcgggcg cgcgaccagc gacaccgggg cacgcgccgg
     1261 gtcgagcacg atgcgcacgg tgcgcgtgaa ctccgcgcgt gcggcgagtg gcgctccgcg
     1321 cagccgttcg atgtcggccg cgctgaagac cgtcgtggct ccgcccgacg cgagcggacc
     1381 ggcgcggacg taaatgtccg caggcagcac ccgcgtgagc cagtcgtcga ccgaggcacg
     1441 gaagctcgcg accatcgtcg ccatcgccac catcagcgcg aacgacgcga cgatgcccgc
     1501 ggccccgatc gatgcgaagc gcggcgtggc cgccagccgc gtggtggcaa gccagcgcgc
     1561 cgcgccgacg ccggtgcggc gcgacgccag gcgcgccagc ggcacgaaca cgtgcggcgc
     1621 gatcagcggc ttgatgaaga tcgcggccgt gagcagcacg gcgatcgaca gataggcaaa
     1681 caccggaatg cccgcgatcg gcggtgccag cagcagcgca ccggcgaaca cgagcagcgc
     1741 caggccgagc cacgcgcgcg ccggtcgcgc atcggcgtcc agacccgagc ccgacttcag
     1801 cgcataggcg ggtgtgtcgc gggcggcgtc gcgcgccggc agccagcccc cgatgatcgc
     1861 cgcggcgacg ccgagcgcga agaaccccgc ggcgttgacg gaatcgaacg ccagcggcgg
     1921 ccgcgcgccg ctgaaataac cgctgccgag atcgcccccc agcagttcga gcgcgaccga
     1981 tgcgatcaac gtgccgagcg cgatgcccag cagcgcgccg acgatgccca gcgcggcggc
     2041 ctcgagcacc agcagcgctt ccacctcgcc gcgcgtggtg ccgagcacgc gcaggaacgc
     2101 gagttgcgcg cgccgcgcca gcacggactg cgactgcagc gagtagacca gaaacgcgcc
     2161 ggtgaacagc gccacgagcg cgagcacgtt caggttcacg cggtacgcgc gcgacaggtt
     2221 cgacacgcgc agcgcggccg catcggccgg ctgcaacgat acgccggcgg gaagctgcca
     2281 gtccttcgcg agcgtcgcga gatcgacgcc cggcgcgagc ttcaggtcga tgtgagtcaa
     2341 ccgtccgagc tgcgccagct tccattgcgc gaacccgatg tccatcgcgg ccacgacggc
     2401 gcccgcgcgc gccgcgggca gtcggcccgc gatgcgcagc gtcaccacgc ggccgccgat
     2461 cagcacgccg atcgagtcgc cgggcgcgac ccggagcgcc tcgaatgcgg ccggagacag
     2521 gtataggccg tcgcccagca gcgcaaggcg atcgtcctgg acgggcgcgc cgatccactg
     2581 cggcgccaac tgcgcggcgc gaaagacgtc gacgccgacg atcgtcaacg tcttgccgcg
     2641 cagtcgttcc ggtgcggcga gcgctacgtc cagcgtgagc atcgggctcg cgagctcgac
     2701 tcgcggatcg gcggcgacac gctcgaccag ccgctcgtcg aacccgccgc tggcgcccgg
     2761 cgcgccgacg atcgaggcgt cggcctggcc gctcacctgg cgcagcgcgg aggagaactc
     2821 cgccagcgcc gagctgttga tcagatgcac cgcgaagccg agtgccacgc cgacggcgat
     2881 cgcgacgacc tggaccgccg cgcgcagcgg atgcgcgcgc gcgtagccgc cgatgacgtg
     2941 tgcaaggagc caacgcatga cggcagtcta tcggcgcggg cttgatgggg gtcgcgacgc
     3001 tctggtgtgc gcgtcgatcg tttgacgctg cgtctccatg cggcgtatcg tgcgcgcctc
     3061 tatttgacgc ccggagaaca gattgggacc cgagcctagt cgcagttgct ccgtcggcgg
     3121 ttccgcacac cggtaggccc cgtgccatgt ccggctttgg atccgatcga cggatatgaa
     3181 gccggtgcag tcaggccgtg accgcggcaa ctttgtccgc cgcatcgatt tgacctgaag
     3241 cgcatcgact ttgctcgtat gcgcgatgct gcgtgcggga tgaacccccg catgccgggg
     3301 agtgatgcat ggtgacgtcc cattcggtag ttgatttctt ccgctccgtg ttcgggctgc
     3361 gcagcgtcgt cgcacggccc gccgcggcca atccgtcgtt cgcgcacctg aacgaaatcg
     3421 cgcaactgtc gaccgaggcg gcactggcgc gactcgacag ttcagacgaa ggactgctcg
     3481 agcgcgagtc gctcgagcgt ctggaacgct acgggccgaa cgaggtcgcc catgagcaga
     3541 aaaaacatcc gctgcggcgc ctgctggaac tcttcgccag cccgctgccg ctgctgctgc
     3601 tggtgctggc actggtggcc gatctcaccg gtgaagtgcg tggcgcgatc gtgatcacgc
     3661 tgatggtcat gctgtcggtg ctgttgtcgt tcgtgcagga attccgttcc agccgtgcgg
     3721 ccgaaaagct gcgcgcgatg gtgcacacga ccgtcaccgt gctgcgcaag gacgtgcgca
     3781 agggtgtgcc cgacgaggtc aatcgctact ttcaggtgcg actgcaatac caggagccga
     3841 agaaggtcga gattccgctg gcccggctcg tgcccggcga tgtggtgacg ctctccgccg
     3901 gcgacatgat cccggcggat gtccgcatcc tgagcgcaaa ggatctgttc gtcagccagg
     3961 ccgcactgac cggcgaatcg ctgccggtcg agaaatttgc cgcgccgtcc aatgccggga
     4021 caacgccgct ggaactcgca aacatcggtt tcatgggcac caacgtcatc agcggcacgg
     4081 ccctggccgt cgtcgcggcc acgggccgca gcacatattt cggttccatt gcagcgacgg
     4141 tgaccggcac gcgtcagctc accagcttcg acctcggcgt caatcgcttc atctggctga
     4201 tgatccgctt catggcggtg atggtgccgc tggtattcgt gatcaacggg ttgtcgaagg
     4261 gcaactggct cgaggcgttc ctgttcgcgg tcgcggtcgc cgtggggctt gcgcccgaga
     4321 tgctgccgat gatcgtcacc atcaatctcg gcaagggcgc gctggcgatg gcgcgcaaga
     4381 aggtgatcgt caagcgtctg aactcgatcc agaacttcgg cgcgatggat gtgctgtgca
     4441 ccgacaagac cggcacgctg acgcaggaca aggtgatcct ggagcggcac gtcgacatct
     4501 tcggcaagga cagcgaccac gtgctgcagt acgcgtatct gaatagctat caccagtcgg
     4561 ggctgaagaa cctcctcgac gtcgcgattc tcaaacacat cgaagtgcac gaagcgctgg
     4621 aggtcaacaa gcactacggc aagaccgacg agattccgtt tgatttccag cgccgccgca
     4681 tgagcgtggt ggtggaaaag gaccacgcca cccacatcct gatctgcaag ggcgcggtcg
     4741 aggagatctt ctcggtctgc accgcggtcg agcgcgacgg cgtgcgcgtg ccgatcgaac
     4801 ccgatcacct cgacgatctg caggaagtga tccgcgacct gaacgacgac ggcttccgcg
     4861 tgattgccgt cgcgttcaag gaagtgccca cgcgcgtgca gccttacgcg gtggccgacg
     4921 agtgcgacct gacgctggtg ggctatgtcg ccttcctcga tccgcccaag gacacggcgg
     4981 cggcggcgct gaaggcgctc gcgcagcatg gcgtgcgcgt caaggtgctg accggcgaca
     5041 acgacgtggt gacgcgcaag gtgtgccgtg acgtggggct cgacacgcag cacgtattgc
     5101 tgggcgccga cctggaaaag ctcaaggatg acgagctcga cgcgcgcgtc gacgaggcga
     5161 ccgtgttcgc gaagctcacg ccgcagcaga aggcgcgcgt gatcggtgcg ctgcatcgcc
     5221 gcggccacgt ggtcggtttc ctcggtgacg gcatcaacga cggaccggcg ctgaaggccg
     5281 ccgacgtcgg catctcggtg gataccgcgg tcgacatcgc caaggaatcg gccgacatca
     5341 tcctgctgga gaagagtctg ctggtgcttg aagccggcgt gctcgagggg cgcgcggtgt
     5401 tcggcaacat caccaagtac atcaagatgg gtgcgagctc gaacttcggc aacatgttca
     5461 gcgtgctcgg cgcgtcggcc ttcctgccgt tcctgccaat ggcgccggtg cagatcctgc
     5521 tcaacaacct gatgtatgac ttctcgcaga cggcggtcgc gaccgacaac gtcgaccgcg
     5581 agtatgtcga gaagccgcgc aagtgggaca tcggcaacat cggccgcttc atgctgttca
     5641 tcgggccgat cagttcgatc ttcgactatg cgacgttcct gacgatgctg tatttgttcg
     5701 acgcgtggac caacgcgccg ctgttccaga ccgcgtggtt cgtcgagtcg ttgctgtcgc
     5761 agacgttgat cgtgcacgtg ctgcgcaccg gcggactgcc gttcgtgcac acgcgtccaa
     5821 gcttgccact ggcgatgacc acgctggcaa tctgtgtact cggcctgtgg ttgccgacct
     5881 cgccctttgc cgaggcgctc ggcttcacgc cgctgccagg cggctgctgg ccggcgctgc
     5941 tggtgatagt tgcggcctat cttggcctga cgcaactcgt gaagacctgg ctcattcggc
     6001 gcttcggtct ggattgactg cgggtgtccg tcagcgggcc gccacaaggc gtccatgttc
     6061 caggcgcagg acgcgatcgg ccgtctgcgc ggcatgcagc gaatgcgtga ccagcacgcc
     6121 ggccgcgccg cggccgcgga tcgtcgcggc gatcaaggcc agcacgctgt cggcggtgtc
     6181 gggatcgaga ttgccggtcg gctcgtcggc cagcaccagc gccggccggt gcaccagcgc
     6241 acgcgccagc gccacccgct gcagttcgcc acccgacagt tcgcgcggcg gcgaccgctc
     6301 tcgacccgcc agcccgaccg cggagatcag gtcgcgcacg cgcgcctcgc gttggtcggc
     6361 atcgacaccc agcagcacca gcggcagctc gacgttctgc gccaccgtca tgtgcgtcag
     6421 gatatggaac gcctggaaca cgaagccgat acggtggcgg cgcagccgcg tgcgcgcgtc
     6481 gtcggacagc tcggatagcg actggccgtc gatgtcgatg cggcctgcgt cgggcaggtc
     6541 gagtccggcg atcaggttca gcagcgtcga cttgcccgat ccggattcgc cgatgatcgc
     6601 aatgagctcg ccgctgccgg cccgcaggtc gatgccgtcg agaagaatgc gggcaccgaa
     6661 gcgcttgcgg acgttggaga gcaccagcat tgcgccgatt ctacggaggc gatgatcact
     6721 acaatcggct gatgcctgcg accgaccccg tcacgatcga cgacatccgc gccgccgccg
     6781 agcgcctgcg cggccaggtc gagaacacgc cgtgcctgca ctcgcgcacg ctgtcgcaga
     6841 tcaccggcgc ggacgtgtac ctgaagttcg agaacctgca attcaccgcc agcttcaagg
     6901 agcgcggtgc actgaaccgc ctcgcccaac tcgacggcga acagaagcag cgcggcgtga
     6961 tcgccgtgtc ggccggcaat cacgcgcagg gcgtggcgta ccacgcgcag cgcatgggcg
     7021 tgccggcggt gatcgtgatg ccgcgcttca cgccgatggt gaaggtcgag cgcacgcgcg
     7081 gcttcggcgc cgagatcatc ctgcgcggcg acaccttcga cgacgcgcgc gaggaggcgc
     7141 tcacgattgc cgccgcgcgc aggctgaccc tggtacaccc gtacgacgac ggtgcggtga
     7201 tcgcagggca gggcacgatc gggctggaga tgctggtcgc gcaaccgcag ctcgactgcc
     7261 tgtgcatggc gatcggcggc ggcgggctca tcagcggggt ggcgatcgcg gcgcgggcac
     7321 tccatccggc gatcgaggtg atcggcgtgc agaccgagca gttcccgtcg atgtacgcgc
     7381 tgttcaaggg catcgagatg ccgtgcgcca acgcgacgct ggccgaaggc atcgcggtca
     7441 agtcgcctgg gctgatcacg cgcgggatcg tgcgcaggat ggtgaacgac atcgtcctgg
     7501 tgtccgaggg cgacatcgag cacgcaatcg tgctgctgct ggagatcgag aagaacgtgg
     7561 tcgaaggcgc cggcgccgca ccgctggcgg cgctgatccg gcatccgcag cgcttcgccg
     7621 gcaagcgggt cgggctggtc ctgagcggcg gcaacatcga tccgatggtg ctcggcgaca
     7681 tcatcgagcg cggcatggtg cgcgcggggc gactggcgcg catccgcgtc gccacgcgag
     7741 acgtgccggg agaactggcg cgagccgcga acatcgtcgg cgcgaccggc gccaacatcg
     7801 aggaagtcga gcaccagcgt gcgttcacga cgctgccggc gcagaacgcg cagctcgaga
     7861 tggtgctgca gacgcgggga ccggagcaca tcgaggagat cctgcgcgca ctgcgcgacg
     7921 ccggcctgcc ggccgaactg cacacgtatt gaacgaacgg gtttgcggtc ggccaaaccg
     7981 gtgcctcgcg cgagtgccgg cgtttgcgtt aggtcagcgc ggctgtctgc gcgcggcgtt
     8041 agcttgcacg catgacggtg gccgcgcgga ccagcaacag gaagacgggg gcggcagttg
     8101 cgcgcaaggg aatggcgcgc agccgcaccg gctgggaagt gaagctgcgc cccgaactgc
     8161 agccgcgcgt ggtgcgcgat ccgaggcagg gcgatcaact cctgattccg accccgctgc
     8221 tggtcgcgga ggaagtgggg cgcgtcgcgc gcggcaaggt gctgacgcta tcggaactgc
     8281 gcaccagcct cgcaaagcgc ttcaaggccg atcgcacctg cccgatgacc accggcatct
     8341 tcgctgcgat cgtcgccggc gcggttcgcg aggacatcgc ccgccggcgc aagccgcgct
     8401 ggccgatctg gcgcctggtg cgcgacgacg gatcgttgaa tccgaactgg ccgttgagcg
     8461 gccgttatcg ggcggcgttg ctgcgggacg agggaatgac ggttacgcgc gctggcacga
     8521 cctggcgtgc cttggtggtc gtcgcagacg aaagcagaaa gaacagccgc gtcgcagtgc
     8581 gacacatgtg acggatgacg gcggtgggat gcagtcgatc tcccgctgaa aaaagcaagg
     8641 gccggttgca ccggcccttc gctcgtgccg ccgaaccgtg gagcgcggcg aactcgtcag
     8701 acgaacgaga agttcgccat gcctccgccg acgtaggcca gttcagtgac cgtcagttcg
     8761 gcgagtgagg attcctgctc ttcggtttcg acctgttcag cgggcacccg tgcggcattc
     8821 atgtggatct ccaggcaatc gttgcgagcg ctcagacaaa ggagaagttg gccattccgc
     8881 cgccgacaag ggcgagttcg gccacctgaa gttcggtcac cagcgcgata tctttggtct
     8941 ctgcggtttc cggcgcggat tgaatcagcg tgtcgttcat gtcagcctcg atctgcgtga
     9001 ggagtggttg gtcaaacgaa cgagaaattg gccatgccgc cgccaacgag cgacagctcg
     9061 acggcgcgca gttcgttcaa ctcttcttgc gcgtcgactt cctgctgtac gactggttcg
     9121 ctcgactgga tcagggtggc gttcatggtg cttccttacg gtagtggacg atcggatttc
     9181 ttcctgccag cgtcgctgta gacagtggcc ccggtgcgtg attaacttcg gtttcctccc
     9241 gtcgcgccat tactaatttc aggtacgcat cgcagcaatg caggatcagc tgtcccgaat
     9301 aagcgagttc tcccctcgcg cgagtaccgc aacgccggaa gaagccctgg cttggctcga
     9361 cgagacatcc cgtatcttgg ctaccgcatt ggcgggtccg ggaccccaga caccgttgtt
     9421 gttttcaaac gcgcacgccg tcctgcggca gctttcgccc cagatcgtca cccggtcgcg
     9481 ggtcgatgcc ctgctgtcgg ttgcccagtt cctctatgtc tcgggccagc cgttcgccgg
     9541 catcgaaccg gccgcggaat ccgtttcgta tgcgcgccgg ctcgagcagc gcggcgaact
     9601 gcgccgcgcg ctgaccttcc acggcatcct gctcgccgac accggcaatc tgccgggtgc
     9661 gatcgaatgc ttcgccgagg cgatcgacgt ggcgcacgcc atcggcgatg tcgtcggcga
     9721 aggctcggcc tggaacaacc tcggcgcggc gttgatctat gcggcgcaat acgcggaggc
     9781 gattcagtgc ctcgagcgag cgatcgagat cgcccagaac gagccgcgcc tggcgaccgt
     9841 tcggctgcag gcgctcagca acgtcgcgct cgcgtgcctg cacctcgagg actacgcgaa
     9901 ggggcttcgc accgccaaga tcgcagtcga atcagcggcg gaaccgaagt cgccgaccga
     9961 gcttctgtcg cgggtgctga gcgagagcta ctacacgcgc ctgctgctcg aagtcgacag
    10021 cctcgagcgc gccaaggaac gctgcgaact cgccaagcgc ttcgccaagg agtcacgctc
    10081 cgaacgtgcc gaactgatcg ccgcgctgtc ggagggactg tacgaagtcc acgccggcat
    10141 gatcgacgtc gggctgtccc gcctgacgaa tgcgctggag cgggcacgga tcctgaagtc
    10201 cgcgctgcgc gacgcgctgg cggcgatggt caaggcgcac gaggtcgccg gcaagccgga
    10261 tctggcgctg gtctacctgc gcgagctgat gctgcacatc aagaaggcgc agcaggacaa
    10321 tgcgctgcgg catcaccagc tgcacatcga gaagctggag cgacagcagc aggccgcggc
    10381 atcggccgag gtggtcatgg agcgccgcga gcagcagttg cgcggccagt tcgccgagca
    10441 ggtcgcgcag caggagctga tgcgctcgcg catcgagatg ctcgagcggc tcgcggtgac
    10501 tgccgagctg cgcgatgact cgaccgggga acactcgtac cgcgtcggca aactggcggc
    10561 gctgctggcg gccgagttcg gctgcgacga gcagacgtgc ttcatgatcg atctggcggc
    10621 gcggctgcac gacatcggca agatcggcat tcccgacggc attctgctga agcccgaccg
    10681 gctcaccgac gccgagcggc agatcatgca gacgcacacc accgtgggcg cggaactgct
    10741 ggcccagagc aacattccgc acatgcagat ggccgaggag atcgcgcgct tccaccacga
    10801 gtggtgggac ggctccggct atccgttcgg catcggcggc agcgcgattc cgatcgcgcc
    10861 gcgcatcacg gccctggcgg acgtattcga cgcgttgaca cacaaacggc cgtacaagga
    10921 gccgtggccg gtggcgcgtg cgctggagga gatcgcgtac ctgaagggtc accagttcga
    10981 cccgcagttg acggaccttt tccttgcgct gatcccgcgc ctgcagcgcg aacagggcga
    11041 tctggacgaa ttcctcggcc gctcggcgcg ccagtccccg ttcatccagg cgcgcgagaa
    11101 gatcgcggcc acgctgcggc aggcgagcgg ccgcgggatg ggcgcgcgcc agcgcttcga
    11161 cgctcagaga tagggcgagc tgcccgcgcg cggtccgagc gcgacgagcc ggtccacggc
    11221 gcgcaccgtc tccggtcggt ggctgacgat gaccagtccc atgccgaggt gcttcaggcc
    11281 gtcggtgatc tcgcgctcac gcgccagatc gagctggtcg aacgcctcgt cgaggaacag
    11341 agcctgcggc cggcgataca gcgcacgcgc caggatcagc cgctgctttt gaccgccgga
    11401 cagcgcgatg cccatgctgc cgacgatcgt gttgtactgc atcggcatcg cctcgatgtc
    11461 ctcgtgaatg cgcgccagcc gcgcgcactc gcgcacgcgt tcggcgtcgt gctgcggatc
    11521 gaagaagctg atgttgtcct cgatcgtgcc gacgaacagc tggtcgtcct gcatcacgac
    11581 gccgatcctg ccgcggtacg cggcgtggtc ccagtcgcgc gc
//
LOCUS       DBNHBY010000007         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1353932, whole genome shotgun sequence.
ACCESSION   DBNHBY010000007 DBNHBY010000000
VERSION     DBNHBY010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1353932"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16HB_00255"
     CDS             <1..660
                     /locus_tag="AC16HB_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5605248.1"
                     /translation="LAAMRRLPSYMANLYAGQWQRSVPARPDWPLAGFGETVAGKTLG
                     IWGYGRIGRLVAGYGRAFGMRVLVHGRQQSLEQAATDGFRATMDREAFFSESDVVTLH
                     LRLVDATRNCVTARELAAMKPTALLVNTSRAELIEPGALVTALARGRPGLAAIDVFER
                     EPVTADEPLLHLPNVICTPHLGFTERQSYEQLLGGAFANIVSFDRGAPANIVNSQVLL
                     K"
     gene            complement(705..911)
                     /locus_tag="AC16HB_00260"
     CDS             complement(705..911)
                     /locus_tag="AC16HB_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5605249.1"
                     /translation="MPTGTVKWFNETKGFGFIKPDDGGADLFAHYSGIKAKGFRTLKE
                     NQRVEYQVQQGQKGPQAVEINPIS"
     gene            complement(1170..>1594)
                     /locus_tag="AC16HB_00265"
     CDS             complement(1170..>1594)
                     /locus_tag="AC16HB_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605250.1"
                     /translation="QFIDARDLARWIIELVQRDIFGIFNAVGPARPLTLAEFLGECAA
                     ALAADAKPVWVATDFLSQQNVQPWTDLPLWAGEEARGVAQVDGARARAAGLVLRPLPD
                     TVHDTWSWICSSRKTAAGGLSAAREADLLARWVQQPAR"
BASE COUNT          298 a          527 c          497 g          272 t
ORIGIN      
        1 ctcgcggcca tgagacgcct gccttcatac atggcgaatc tctacgcagg tcaatggcag
       61 cgcagcgtgc ccgcgagacc ggactggccg cttgcgggct tcggcgagac cgtcgccgga
      121 aagacgctcg gcatctgggg ctacgggcgg atcgggcgcc tggtcgcagg gtacggccgc
      181 gctttcggca tgcgcgtctt ggtccatggc cgccaacagt cgctggaaca ggcggccacc
      241 gacggcttcc gcgcgaccat ggaccgcgag gcgttcttca gcgaaagcga cgtcgtcacg
      301 cttcacctgc ggctggtcga cgccacgcgc aactgcgtga ccgctcgtga gctcgccgcg
      361 atgaaaccaa ccgcactcct cgtcaacacg agccgcgccg agctgatcga accgggtgcg
      421 cttgtcaccg cgcttgcgag gggccgcccg ggattggcgg ccatcgatgt cttcgagcgc
      481 gagccggtca cggcggacga gccgctgctg cacctgccaa acgtcatatg cacgcctcat
      541 ctcgggttca ccgagcgaca gtcatatgag caattgctcg gcggcgcatt cgcgaacatc
      601 gtgtcctttg accgcggcgc gccggcgaac atcgtcaaca gccaagtgct gctcaaataa
      661 aaagggccgg tcattcgacc ggcccctcgt gcggcaatcc cggatcaact gatcggattg
      721 atctcgacgg cctgcgggcc cttttgaccc tgctggacct ggtactcgac ccgctggttc
      781 tccttcagcg tccggaaccc ttttgccttg attccgctgt agtgcgcgaa caagtcggcg
      841 ccgccgtcgt ccggcttgat gaacccgaaa cccttcgtct cattgaacca tttcacggtg
      901 cctgtcggca tagaactctc acatggaata acacgggaag cgcccgcggc gcgcaagcga
      961 ttggtgcgga atagctgggg agtactgcga caggaacttc aatgggaaca aggcgcctga
     1021 agcgcgaatg ggcttagatg ccttgagacc gagcgagcac ttggagccag ataacacggc
     1081 gcagaaatcg actgcggaag caaagctacc ccaatcggcg cgcccgcgct agaggggatc
     1141 ggtgtgcgct ggcgccgatc ggactcacgt caacgcgcag gttgctgcac ccatctggcc
     1201 agtagatcgg cctcgcgcgc tgccgatagt ccgccggcgg ccgtctttct ggaactgcag
     1261 atccaggacc aagtgtcgtg cacggtatcg ggcagtgggc gaagaaccag acctgcggcc
     1321 cgagcacgcg caccgtccac ctgcgcaacc ccgcgtgctt cttcgcctgc ccatagcggc
     1381 agatcagtcc atggttggac gttctgctga ctcagaaagt cggtggccac ccacaccggc
     1441 ttcgcgtcgg cggcgagcgc ggcggcacac tcgcccagaa attccgccag ggtcaacggc
     1501 cgcgctggcc ccacggcgtt gaagataccg aagatgtcgc gttgcacaag ctcgatgatc
     1561 caacgcgcca ggtcccgcgc gtcgatgaac tgca
//
LOCUS       DBNHBY010000008         1661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_677119, whole genome shotgun sequence.
ACCESSION   DBNHBY010000008 DBNHBY010000000
VERSION     DBNHBY010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_677119"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="AC16HB_00270"
     CDS             <1..922
                     /locus_tag="AC16HB_00270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5605251.1"
                     /translation="AQVLGVGDHALAQIGAVPLLLARGDDGQLRALHNVCRHRAGPLA
                     ECDGRGAKSLVCRYHGWTYALDGTLRGAPEMGRARDFDPSSIRLPEARVAQWQGLVFA
                     ALADAATVFDDVVAGIDARLPQPLTQHAFDRRVSYEVACNWKVYVDNYLEGYHVARVH
                     PRLATVLDGTQYVTRVARWHSLQSSPLADTRPYGAGEALYHFVYPNTMLNTLPGRLQT
                     NRVVPLAADRCRVEFDYFYSPSVAAAQRLEDQRFSDEVQCEDIAICEAVQRGLASGSY
                     TPGRLNPHAESGVHHFHELLRADYRAASVR"
     gene            complement(898..>1661)
                     /locus_tag="AC16HB_00275"
     CDS             complement(898..>1661)
                     /locus_tag="AC16HB_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5605252.1"
                     /translation="GNRHLAVDDDHYLILNERQRYGSRLRSLRPVGSMSIFFRPGMPR
                     EVAAALSQSEAQALDGGPTTAGPPFAFAEHLRPHDATITPLLRSLRREIQAGQDDDDW
                     VEEQLQRLCAALIRAEPGYRRRAQRLAPFSRSTQGELLARVDRAADFMLTCYARRISL
                     DDIAAAAPLSKFHLVRLFRRVHGVTPYAFLLRKRLAVARRLLAGTDLSISEIAAQSGF
                     GTRFTLFRQLRAADGRSGRAWRERGGGAYRTDAAR"
BASE COUNT          245 a          593 c          585 g          238 t
ORIGIN      
        1 ggcgcaggtg ctgggcgtgg gcgatcacgc gctggcacag atcggcgcgg tgccgctgct
       61 gctcgcgcgt ggcgacgacg gccaactgcg cgcgctgcat aacgtgtgcc gacatcgggc
      121 cgggccgctg gcggagtgcg acggccgcgg cgcgaagtcg ctggtgtgcc gctatcacgg
      181 ctggacctat gcgctcgacg gcacgctgcg cggcgcgccc gagatgggtc gcgcgcgcga
      241 cttcgatccg tcttcgatcc gcctgcccga ggcacgcgtc gcgcaatggc agggactcgt
      301 gttcgccgcg ctggccgatg ccgcaaccgt cttcgacgat gtcgtcgcgg gcatcgacgc
      361 gcgcctgccg cagccgctca ctcagcacgc cttcgaccgc cgcgtcagct acgaggtcgc
      421 gtgcaactgg aaggtgtacg tcgacaacta cctcgagggc tatcacgtgg cacgcgtgca
      481 cccgcggctc gcgaccgtgc tcgacggcac gcagtacgtc acccgcgtcg cgcgctggca
      541 ttcgctgcag tcgagcccgc tcgccgatac gcgaccgtac ggtgccggcg aagcgctgta
      601 ccacttcgtc tatccgaaca cgatgctgaa cacactgcca gggcggctgc agaccaaccg
      661 cgtggtgccg ctggcagccg atcgctgccg cgtcgagttc gactacttct actccccgtc
      721 cgtcgccgcc gcgcagcggc tggaggatca gcgcttcagc gacgaggtgc aatgcgagga
      781 catcgcgatc tgcgaagcgg tgcagcgcgg gctcgcgtcg ggctcataca cgccggggcg
      841 gctgaacccg cacgccgagt ccggcgtgca ccacttccac gaactgctgc gcgccgacta
      901 ccgcgcggcg tccgtccggt aggcgccgcc gccgcgttcg cgccaggcgc ggccgctgcg
      961 tccgtcggcc gcgcgcaact ggcggaacag cgtgaagcgc gtgccgaagc cgctctgtgc
     1021 ggcgatctcg ctgatcgaca gatcggtgcc cgccagcagg cgccgcgcca cggcaaggcg
     1081 tttgcgcagc aggaacgcat acggtgtcac gccgtgcacg cggcggaaca gccgcaccag
     1141 atggaacttc gacaacggcg ccgcagccgc gatgtcgtcg agcgagatgc gccgcgcgta
     1201 acaggtcagc atgaagtcgg ccgcgcgatc gacgcgcgcc agcagttcgc cctgcgtgga
     1261 gcgggagaac ggcgccagcc gctgcgcgcg ccggcggtag cccggctcgg cgcggatcag
     1321 cgcagcgcac agccgctgca attgctcctc gacccagtcg tcgtcatcct gcccggcctg
     1381 gatctcgcgc cgcagcgagc gcagcagcgg cgtgatcgtc gcatcgtgcg gtcgcaggtg
     1441 ctcggcgaac gcgaacggcg gccccgccgt cgtcggcccg ccgtctagcg cctgcgcctc
     1501 cgactgtgac agcgcggcgg cgacttcgcg cggcatgccg gggcggaaga agatgctcat
     1561 gctgccgacc gggcgcaggc tgcgcaggcg cgatccatac cgttgccgtt cgttcaggat
     1621 cagatagtgg tcgtcgtcga ccgcaagatg gcgattgccg a
//
LOCUS       DBNHBY010000009         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1556831, whole genome shotgun sequence.
ACCESSION   DBNHBY010000009 DBNHBY010000000
VERSION     DBNHBY010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1556831"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16HB_00280"
     CDS             <1..390
                     /locus_tag="AC16HB_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR5605253.1"
                     /translation="LLRTDFRTPLGTVSYHVPCHGRVQNIGRKTEELLKLVPQTTLNT
                     VERCSGHSGLWGTRKQFHEMAMKIGKPVFKSMAGAKPDYVSSDCQLAGHHLQQGIERF
                     AKEALDGKQPALAHPITLVRIAYGLEG"
     gene            405..989
                     /locus_tag="AC16HB_00285"
     CDS             405..989
                     /locus_tag="AC16HB_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR5605254.1"
                     /translation="MPRIERDSLLTLEAYARQRKEFRTRVIAHKKHRTVRLGEHVTLI
                     FEDELTVRYQIQEMLRIEKTFEEDGIQDELDAYNPLIPDGTNLKATMMIEYEDPVVRA
                     RELAQLKGIEAAVYVQVDGHSKVNAIADEDLERENEEKTSSVHFLRFEFSPAMIAAFK
                     GGAAVAMGVDHPHYRARVDEIAPEVQAALARDFS"
     gene            1022..2134
                     /locus_tag="AC16HB_00290"
     CDS             1022..2134
                     /locus_tag="AC16HB_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR5605255.1"
                     /translation="MQRKRISQAPVHIVPRKGRGATFNVAHRFERVEREADGDALDAA
                     RLAEPDDALLPLKTEVKCEQARSILAWNDSPDIYFDRSINPYRGCEHGCVYCYARPTH
                     SYLGLSPGLDFETKLVAKTNAAELLKRELAAPGYHADVINLGSATDAYQPVERELRIT
                     RQILEVLAAARHPVAIVTKSSLVERDLDLLVPQARQRLVAVYVSVTTLDHELSRRLEP
                     RAASPTRRLRTIETLARAGVPVCVNVAPIIPFVTEPDMERIMQFAAGAGAAAAFYTVL
                     RLPWEVNPLFQDWLAKHYPERAQRVMNRVRDMRHGKDYDAAFKTRMKGEGVWADLIRN
                     RFDKACARLGLQFKRFDLRTDLFVPPARDERQQSLF"
     gene            complement(2131..>2375)
                     /locus_tag="AC16HB_00295"
     CDS             complement(2131..>2375)
                     /locus_tag="AC16HB_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605256.1"
                     /translation="NWYQTPAFFVIVVPFIAGCIEAIVLALTPDAKFDARFNPTSEQT
                     NRSGWDAVLVAIATLVVGSIVLMATIALFFQTLFER"
BASE COUNT          450 a          781 c          777 g          367 t
ORIGIN      
        1 ctgctgcgga ccgatttccg caccccgctc ggcaccgtgt cctaccatgt gccgtgccac
       61 gggcgggtgc agaacatcgg ccgcaagacg gaagagctgc tcaagctggt gccgcagacg
      121 acgctcaaca ccgtcgagcg ctgctcggga cattcgggtc tgtggggcac gcgcaagcag
      181 ttccacgaga tggcgatgaa gatcggcaaa cccgtattca agtcgatggc gggcgcgaaa
      241 cccgactacg tcagctccga ctgccagctc gccggtcacc acctgcagca ggggatcgag
      301 cgcttcgcga aggaagcgct cgacggcaag caacccgctc tcgcgcaccc gatcacgctg
      361 gtgcgcatcg cctacggact ggaaggatga tggagaacgc tgacatgccg cgcatcgaac
      421 gggacagcct gctgacgctt gaggcctacg ccaggcaacg caaggaattc cgcacccgcg
      481 tgatcgcgca caagaagcac cgcaccgtgc gcctgggcga gcacgtaacg ctgatcttcg
      541 aggacgaact gacggtccgc taccagatcc aggaaatgct gcggatcgag aagaccttcg
      601 aggaggatgg gatccaggac gagctcgacg cgtacaaccc tctgatcccc gacggaacga
      661 acctgaaggc gaccatgatg atcgagtacg aggacccggt cgtccgcgcg cgcgagctgg
      721 cgcagctcaa aggcatcgag gccgcagtct atgtgcaggt cgacggccac tcgaaggtga
      781 atgcgattgc cgacgaggac ctggagcgcg agaacgagga gaagacctcg tcggtgcact
      841 ttctgcgctt cgagttctcg cccgcgatga tcgccgcttt caagggcggt gctgcggttg
      901 cgatgggggt cgatcacccg cactaccgtg cccgcgtcga cgagattgcc cccgaggtgc
      961 aagcagcgct ggcgcgggac ttctcgtagc gctggcggcg ctggttgcga tcatctcgcg
     1021 catgcagcgc aagcggattt cgcaggcccc ggttcacatc gtgccgcgca agggccgcgg
     1081 cgcgaccttc aacgttgcgc atcggttcga acgtgtcgag cgcgaggccg acggcgacgc
     1141 gctcgacgcc gcccgtctcg cggaacccga cgacgcgctg ctgccgctga aaaccgaggt
     1201 gaagtgcgag caggcccggt cgatcctcgc ctggaacgac tcgcccgaca tttacttcga
     1261 ccgctcgatc aatccgtacc gcggctgcga gcacggctgc gtctactgct acgcgcggcc
     1321 gacgcacagc tatctgggac tgtctccggg gctcgacttc gagacgaagc tggtcgcaaa
     1381 aacgaatgcg gccgagttgc tcaaacgcga actcgccgcg ccgggctatc acgccgacgt
     1441 gatcaacctc ggctcggcga ccgatgccta tcagccggtc gagcgcgagt tgcgcatcac
     1501 tcggcagatc ctcgaagtgc tcgccgccgc gcggcatccg gtcgcgatcg tcaccaagtc
     1561 gagccttgtc gagcgcgacc tggatctgct ggtgccacag gcgcggcagc gactggtcgc
     1621 cgtctacgtg agcgtgacca cgctcgatca cgagctgtcg cgcaggctcg agccgcgcgc
     1681 cgcgagtccg acgcgccgat tgcggacgat cgaaacgctc gcgcgcgccg gcgtgccggt
     1741 gtgcgtgaat gtcgcgccga tcatcccttt cgtgaccgag ccggacatgg agcgcatcat
     1801 gcagttcgcg gccggcgccg gcgcggcggc ggcgttctac acggtgcttc gcctgccgtg
     1861 ggaggtcaac ccgctgttcc aggactggct cgcaaagcac tatccagaac gcgcgcagcg
     1921 ggtgatgaac cgcgtgcgcg acatgcgcca cggcaaggat tacgacgcgg cgttcaagac
     1981 tcgcatgaag ggcgagggtg tgtgggcgga cctgatccga aaccgcttcg acaaagcgtg
     2041 cgcgcggctc gggctgcagt tcaagcgctt cgacctgcgc accgatctct tcgtgccgcc
     2101 cgcccgcgac gaacgccagc agagcctctt ctaacgctcg aacagggtct ggaagaacag
     2161 cgcgatcgtc gccatcagca cgatcgatcc gaccaccagc gtcgcaatcg cgaccaggac
     2221 cgcgtcccag ccgctgcggt tggtctgctc ggacgtcgga ttgaagcgcg cgtcgaactt
     2281 cgcgtcgggg gtcaacgcaa gcacgatcgc ctcgatgcag ccggcgatga acggaacaac
     2341 gatcacgaag aaggcgggcg tctggtacca gttct
//
LOCUS       DBNHBY010000010         1546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_406520, whole genome shotgun sequence.
ACCESSION   DBNHBY010000010 DBNHBY010000000
VERSION     DBNHBY010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_406520"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="paaC"
                     /locus_tag="AC16HB_00300"
     CDS             <1..454
                     /gene="paaC"
                     /locus_tag="AC16HB_00300"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR5605257.1"
                     /translation="LYTSFQAQLWQGLAASADEQLAAIAAKSAKETRYHRQHSADWAA
                     RLGDGTEESHARMQRALDYLWPYTAELFAATREDDEVAAAQLGVAWPTLQAAWEADVL
                     PVLAQATLRVPARTPFLSRGKLGVHTEHMGHLLTEMQYLQRAYPGGTW"
     gene            460..948
                     /gene="paaD"
                     /locus_tag="AC16HB_00305"
     CDS             460..948
                     /gene="paaD"
                     /locus_tag="AC16HB_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR5605258.1"
                     /translation="MTVERAWDVLARVADPEIPVISVTELGIVRDVCAREDSIEVVVT
                     PTYSGCPATELIARSIEQALHEAGATNVRVRQRLAPAWTTDWIAPAAKDKLARYGIAP
                     PGSVGEQPVERRIRFLPRTIACPRCGSNDTERLSQFGATACKALHRCRACREPFEYFK
                     PI"
     gene            945..>1546
                     /locus_tag="AC16HB_00310"
     CDS             945..>1546
                     /locus_tag="AC16HB_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5605259.1"
                     /translation="MTPRFHTLRIAEVRRETADAVSVRFEVPQDLVSEYRFKPGQHLN
                     LRARLDGEEMRRSYSICSGLDDGELRVAIKKVAGGRFSAWANDTLKPGDTIDVMTPEG
                     RFHLPRDASRARHYVGFAAGSGITPILSLVKTILAREPRSRFTLIYGNRRQSSVMFHE
                     ALEDLKDRYLTRFALYNVFSREEQEVGLFNGRIDAAKTRA"
BASE COUNT          249 a          524 c          542 g          231 t
ORIGIN      
        1 gctgtacacg tcgttccagg cgcagctgtg gcaggggctc gcggcatccg ccgacgagca
       61 actcgccgcg atcgcggcca agagcgccaa ggaaacgcgc tatcaccgcc agcattctgc
      121 cgactgggcg gcccggctcg gcgacggcac cgaagaatcg cacgcacgga tgcagcgcgc
      181 gctcgactac ctgtggccgt acaccgcgga actgttcgcc gcgacgcgag aggacgacga
      241 agttgccgcc gcgcagctcg gggttgcgtg gccgacgctg caggccgcgt gggaagcaga
      301 tgtcctgccg gtgctcgcgc aggcgacgtt gcgggtgccg gcgcggacgc cgttcctgtc
      361 gcgcgggaag ctcggcgtgc acaccgagca catgggccac ctgctgaccg agatgcagta
      421 cctgcagcgc gcctatccgg ggggaacctg gtgagttcaa tgacggtcga gcgcgcgtgg
      481 gacgtgctgg cgcgcgtcgc cgatccggag atcccggtga tctcggtgac cgaactgggc
      541 atcgtgcgcg acgtgtgcgc gcgcgaagac tcgatcgaag tcgtcgtgac gccgacgtac
      601 tccggctgcc cggcgaccga gctgattgcg cgctcgatcg agcaggcgct gcacgaggcg
      661 ggggccacca acgtgcgcgt ccggcagcgg ctggctccgg catggaccac cgactggatc
      721 gcgcctgccg cgaaggacaa gcttgcacgc tacggcatcg cgccgcctgg cagtgtgggc
      781 gagcagccgg tcgagcggcg gatccggttc ctgccgcgca cgattgcctg cccgcgttgc
      841 ggcagcaacg acaccgagcg gctgtcgcag ttcggcgcca ccgcctgcaa ggcgctgcat
      901 cgctgccgcg cgtgccgcga accgttcgag tacttcaagc cgatatgacc ccccgcttcc
      961 atacgctgcg catcgccgaa gtccggcgcg agacggcgga cgccgtgagc gtgcgcttcg
     1021 aagtgccgca ggatctcgtt tccgaatacc ggttcaagcc cggccagcac ctgaacctgc
     1081 gcgcgcggct cgacggcgag gaaatgcgcc gttcgtactc gatctgcagc gggctggatg
     1141 acggcgaatt gcgggtggcg atcaagaaag tcgccggcgg ccgcttctca gcctgggcca
     1201 acgacacgct gaagccgggc gacacgatcg acgtgatgac gcccgaggga cgtttccact
     1261 tgccccgcga cgcgtcccgc gcccgacact atgtcggctt cgcggccggc agcggcatca
     1321 cgccgattct gtcgctggtg aagacgatcc tggcgcgcga gccgcgctcc cgcttcacgc
     1381 tcatctacgg caaccgccgg cagtcgtcgg tgatgttcca cgaggcgctg gaggacctga
     1441 aggatcgcta cctgacccgc ttcgcgctgt acaacgtgtt ctcgcgcgag gagcaggagg
     1501 tgggtctgtt caacgggcgc atcgacgcgg ccaagacgcg cgcgtt
//
LOCUS       DBNHBY010000011         5265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1692301, whole genome shotgun sequence.
ACCESSION   DBNHBY010000011 DBNHBY010000000
VERSION     DBNHBY010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1692301"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC16HB_00315"
     CDS             complement(<1..812)
                     /locus_tag="AC16HB_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605260.1"
                     /translation="MPAIEAIDVAKRYATRGAGVTALDGVSLRIEHGEFFGLLGPNGA
                     GKTTLISIIAGLARATRGSVAVMGHDVVTDYRSSRRCLGVVPQELVFDPFFTVRETLR
                     IQSGYFGLRDNGAWIDEVMANLDLTGKADANMRALSGGMKRRVLVAQALVHRPPVIVL
                     DEPTAGVDVELRQALWAFVRRLNREGHTIVLTTHYLEEAQELCGRIAMLKAGRVVALD
                     ATRNLLNRLAGVRVSLRLRGAPPEALRARVIEASDGRLALRLAAASEVEGVLA"
     gene            complement(877..1137)
                     /locus_tag="AC16HB_00320"
     CDS             complement(877..1137)
                     /locus_tag="AC16HB_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5605261.1"
                     /translation="MKLDVARISNDNVVTLLEQGLVAIRAGDLAFDLSAVRECNTAAV
                     AMLLAWQREAGARSAKLQLTGLPANLSSLAQLYGVEALVTGG"
     gene            complement(1134..1823)
                     /locus_tag="AC16HB_00325"
     CDS             complement(1134..1823)
                     /locus_tag="AC16HB_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5605262.1"
                     /translation="MMETVMIRNANLIRRLAVGAACVLVSLGVAHAQGTAAANAPASA
                     ATQAPDALVRTLSNDVLDASKKDPSLKAGDLTKLNALVDEKILPYVNFEKMTQLAVGR
                     GWRQASPDQRQRLTKEFRSLLVRTYSGAVSAVTDHQVQLRPYRAQPADTDVMVRTQVV
                     PSRGEPIQLDYRLEKTDAGWKVYDVSVLGVWLVENYKSQFATEIQQNGVDGLIKSLAE
                     RNRQLAEGKKS"
     gene            complement(1863..2660)
                     /locus_tag="AC16HB_00330"
     CDS             complement(1863..2660)
                     /locus_tag="AC16HB_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR5605263.1"
                     /translation="MMRDLRDRTLRLLRLLALGALAVLSGACATIPPTAGSNPVDPLE
                     RYNRHMSDFNERVDRAVLKPVAQAYADYVPAPVRDCVGNIFSNIGDVPSALNNLLQGK
                     PREAVSDICRVVINTTVGVLGCFDVASKAGLEKSGEDFGQTLGRWGVGQGPYFVWPFL
                     GPSTIRDSVGRVVGFYTDPLDYVDNVRWRNSLWATRFVDTRASLLPAEKILEGALDRY
                     QFIRDAYLQRRRKQVYDGNPPREREPDEDAEPAPATAPDQPNPAPTR"
     gene            complement(2665..3141)
                     /gene="mlaD"
                     /locus_tag="AC16HB_00335"
     CDS             complement(2665..3141)
                     /gene="mlaD"
                     /locus_tag="AC16HB_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR5605264.1"
                     /translation="MGRKGIETLVGLFVLAGFVAIGYLALKAANLASFTIGETYTVTA
                     KFDNIGGLKVRAPVRSAGVTVGRVSRITFDNQSFQGIVTMEIEKKFQFPQDTSAKILT
                     AGLLGDQYIGLNPGGDDKNLAAGDTIKMTQSAVVLENLIGQMLYNKAAEGAGDAKK"
     gene            complement(3153..3944)
                     /gene="mlaE"
                     /locus_tag="AC16HB_00340"
     CDS             complement(3153..3944)
                     /gene="mlaE"
                     /locus_tag="AC16HB_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR5605265.1"
                     /translation="MSPSDLLAQLGARALQWLRGLGHSAFFFGELIGSIPAALRRFYL
                     VVAQIHAIGNYSLIIIVASGLAVGFVLGLQGYYTLSRYGSEESLGLLVALTLVRELSP
                     VVTALLYAGRAGTSLTAEIGLMKAGEQLAAMEMMAIDPKQRVLAPRFLGGIVAMPLLA
                     AMFSAIGIFGGYVVGVQMIGIDSGQFWSQMQDGVDVFDDVGNGMIKSTVFGLVCTFVA
                     LYQGFEAEATPEGVARATTRTVVISSLSVLLLDFVLTAVMFSTPR"
     gene            complement(3941..4744)
                     /locus_tag="AC16HB_00345"
     CDS             complement(3941..4744)
                     /locus_tag="AC16HB_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605266.1"
                     /translation="MNSDAFIRIDHVTFGYDQRVILSDVSLTFPRGKVVAIMGGSGCG
                     KTTLLRLIGGVIRPQKGHITFDGEEVDPRNRERLYALRRRLGMLFQFGALFTDLTVFE
                     NVAFPIREHTELPESMIRDIVLMKLHAVGLRGAAHLRIAEVSGGMARRVALARAIALD
                     PELIMYDEPFAGLDPISMGVTANLIRRLNDATGATSLIVSHDVRECFLISDYAYLISA
                     GRIVAHGTPEELNASADPDVRQFIRGEPDGPVPFHYPAPPIAADLGLRA"
BASE COUNT          884 a         1829 c         1713 g          839 t
ORIGIN      
        1 gcgagcacgc cttcgacctc cgacgccgca gcgagccgca gcgcgaggcg gccgtcgctg
       61 gcctcgatca cgcgcgcacg cagtgcctcg ggcggcgcgc cgcgcaagcg caggctgacg
      121 cgcacgccgg cgagccggtt cagcaggttc cgcgtcgcat cgagtgcgac gacgcggccc
      181 gccttcagca tcgcgatacg gccgcacagt tcctgcgcct cttccaggta gtgcgtggtc
      241 agcacgatcg tgtgcccttc ccggttcagg cgccgcacga acgcccacag cgcctgccgc
      301 aactcgacgt ccacaccggc cgtgggctcg tcgagcacga tgaccggcgg ccgatgcacc
      361 agcgcctgcg cgaccagcac gcgccgcttc atgcctcccg acagcgcgcg catgttggcg
      421 tccgccttgc cggtgagatc caggtttgcc atgacctcgt cgatccaggc gccgttgtcg
      481 cgcaggccga agtagcccga ctggatgcgc agcgtctcgc gcacggtgaa gaacggatcg
      541 aacaccagtt cctgcggcac cacgccgagg cagcggcgtg agctgcggta gtcggtcacc
      601 acgtcgtgtc ccatgaccgc gacggatccg cgcgtggcgc gcgccagccc ggcgatgatc
      661 gagatcagcg tggtcttgcc ggcgccgttg ggaccgagca gcccgaagaa ctcgccgtgc
      721 tcgatgcgca ggctgacgcc gtcaagcgcg gtgacgcccg cgccacgggt cgcgtagcgc
      781 ttggcgacat cgatggcttc gatcgcgggc agcggcatgg acgaaaacgc caccgccccg
      841 taggtcgggg cggaatcgca attctacggg agcgggtcag ccgccggtga cgagcgcctc
      901 gacgccgtaa agctgcgcga ggctggacag attggccggc aggcccgtca actgaagctt
      961 cgcgctgcgc gcaccggcct cgcgctgcca ggcgagcagc atcgccaccg ccgcggtgtt
     1021 gcactccctg acggcggaca ggtcgaacgc gaggtcgccg gcgcgtatcg cgaccaaacc
     1081 ctgttcgagc agggtcacga cgttgtcgtt cgagatgcgc gcaacgtcga gcttcaagac
     1141 ttcttgcctt ccgcgagctg gcgattgcgc tcggcgagcg acttgatcaa gccgtcgacg
     1201 ccgttctgct ggatctcggt ggcgaactga ctcttgtagt tctcgaccag ccacacgccg
     1261 agtacgctga cgtcgtagac cttccagccg gcgtccgtct tctccagccg gtagtcgagt
     1321 tggatgggtt cgccgcgcga tggcacgacc tgcgtgcgca ccatcacgtc ggtatcggcc
     1381 ggctgtgcgc gatagggccg cagctggacc tgatgatcgg tcaccgccga tacggcgccg
     1441 ctgtaggtcc ggaccagcag agagcgaaat tccttggtca ggcgctggcg ctggtcgggc
     1501 gacgcctgac gccaaccgcg gccgaccgcc agctgcgtca tcttctcgaa gttcacgtac
     1561 ggcagaatct tttcgtcgac gagcgcgttc agcttcgtca ggtcgcccgc cttcagcgac
     1621 ggatccttct tgctcgcatc gagcacatca ttcgacagcg tgcgcaccag cgcatcgggc
     1681 gcctgcgttg ccgcgctcgc gggcgcgttt gcagccgccg ttccctgcgc gtgcgccaca
     1741 cccagcgaca cgagcacgca tgcagcaccg acggcaaggc gtcgaatcag gtttgcgtta
     1801 cggatcatca cagtttccat catcgagacc tcatcgaagc cggacagttc cggaccgtgc
     1861 ccttaacgcg tcggcgcagg gttcggttga tccggggccg tcgccggagc cggctctgcg
     1921 tcctcgtccg gttcgcgctc gcgcggcgga ttgccgtcgt agacctgttt gcggcgacgc
     1981 tgaaggtagg cgtcgcggat gaactggtag cggtcgagcg cgccctcgag gatcttttcc
     2041 gcaggcaaca ggctcgcgcg cgtgtcgacg aagcgcgtcg cccacagcga attgcgccag
     2101 cgcacattat cgacgtagtc gagcggatcg gtatagaaac cgaccacacg cccaaccgag
     2161 tcgcggatcg tgctcgggcc gaggaacggc cagacgaaat acggcccctg ccccaccccc
     2221 cagcggccga gcgtctgacc gaagtcctcg ccgctcttct cgagtcccgc cttgctggcg
     2281 acatcgaagc agccgagtac gcccaccgtt gtgttgatca ccacgcggca gatgtccgac
     2341 accgcctcgc gcggcttgcc ctgcagcaga ttgttcagtg cgctcggcac gtcgccgata
     2401 ttgctgaaga tgttgccgac gcagtcgcgc accggcgccg gaacgtagtc cgcatatgcc
     2461 tgcgcgaccg gcttgagcac cgcgcgatcg acgcgctcgt tgaagtccga catgtggcgg
     2521 ttgtagcgct ccagcggatc gaccggatta ctgcccgcag tcggcggaat cgtcgcgcac
     2581 gcgccgctca acacggccag cgcgcccagc gcgaggagcc gcagcaatcg cagcgttcga
     2641 tcgcggaggt cgcgcatcat cgtctcactt cttcgcgtcg cccgcgccct cggccgcctt
     2701 gttgtacagc atctggccga tcaggttctc cagaaccacc gccgactgcg tcatcttgat
     2761 cgtgtcgccc gccgcgagat tcttgtcgtc gccgccgggg ttcaggccga tgtactggtc
     2821 gccgagcagg cccgccgtca ggatcttcgc cgacgtgtcc tgcggaaact ggaacttctt
     2881 ctcgatttcc atcgtcacga tgccctggaa agactggtta tcgaacgtga tccgcgacac
     2941 gcgtccgacc gtgacgccgg cgctgcgcac cggggcgcgc accttgagcc cgccgatatt
     3001 gtcgaacttc gcggtcacgg tgtacgtctc cccgatcgtg aagctggcca ggttggccgc
     3061 cttcagcgcc aggtagccga tcgcgacgaa gccggccagc acgaacagac cgaccagcgt
     3121 ttcaatgccc ttgcgtccca tcgttcactc cgtcaacgcg gcgtcgagaa catgaccgcc
     3181 gtcagcacga aatcgagcag cagaacggac agcgacgaaa tcaccaccgt gcgcgtggtg
     3241 gcccgcgcca cgccttccgg cgtggcctcg gcctcgaagc cctgatacag cgcgacgaag
     3301 gtgcacacca ggccgaatac cgtgctcttg atcatgccgt tgcccacatc gtcgaatacg
     3361 tcgacgccat cctgcatctg cgaccagaac tggccggagt cgatgccgat catctgcaca
     3421 ccgaccacgt aaccgccgaa gatgccgatc gccgagaaca tcgccgccag cagcggcatc
     3481 gcgacgatgc cgccgaggaa gcgtggcgcc agcacgcgct gcttggggtc gatcgccatc
     3541 atctccattg cggccagctg ctcgcccgcc ttcatcaggc cgatctcggc ggtcagcgaa
     3601 gtgccggcgc ggccagcgta cagcagcgcg gtgaccaccg gcgacagctc ccgcaccagc
     3661 gtgagcgcaa ccagcagccc gagcgactcc tccgatccgt agcggctcag cgtgtagtag
     3721 ccctgcaggc cgagcacgaa gccgaccgcc agccccgagg caacgatgat gatcagcgag
     3781 tagttgccga tcgcgtggat ctgcgccacc accaggtaga agcgccgcag cgcggccgga
     3841 atgctgccga tcagctcgcc gaagaagaac gcgctgtgac cgagcccgcg cagccactgc
     3901 agcgcgcgcg cgcccagctg cgccagcagg tcgctcgggc tcacgcgcgc aacccgaggt
     3961 cggccgcgat cggcggcgcg gggtagtgga acggcaccgg gccgtccggc tcgccgcgga
     4021 tgaactggcg cacgtcggga tcggccgacg cgttcaactc ctccggcgtg ccgtgcgcga
     4081 cgatgcgccc ggcggagatc aggtacgcgt agtcgctgat caggaagcac tcgcgcacgt
     4141 cgtgcgacac gatgagcgag gtcgcaccgg tggcgtcgtt cagacgccgg atcaggttgg
     4201 cggtcacgcc catcgagatc gggtcgagcc cagcgaacgg ctcgtcgtac atgatcagct
     4261 ccgggtcgag cgcgatcgcg cgtgccagcg caacgcggcg cgccatgccg ccggacacct
     4321 cggcgatgcg caagtgcgcg gcgccgcgca gcccgaccgc gtgcagcttc atcagaacga
     4381 tgtcgcggat catcgactcc ggcagttcgg tgtgctcgcg gatcggaaac gcgacgttct
     4441 cgaacaccgt gagatcggtg aagagcgcgc cgaactggaa caacatgcca agccggcggc
     4501 gcagcgcgta caaacgctcg cggttgcgcg ggtcgacttc ttcgccgtcg aaggtgatgt
     4561 gacccttctg cggccggatc acgccgccga tcagccgcag cagcgtggtc ttgccgcaac
     4621 cggacccgcc catgatcgcc accaccttgc cgcgcgggaa ggtgagcgac acatcggaca
     4681 ggatcacgcg ctggtcatag ccgaacgtga cgtgatcgat gcggatgaag gcgtcggaat
     4741 tcacgacgga cgatcgcccc gtattgatct acttgcttgt tgttcaggac agacaaggcc
     4801 ggcgactcgc cgacacggcg ggccggtgtg cccgctcaat caccgctcgt gcggtgcagc
     4861 ggcgcggcga ttgtcgcaca gcggacccgc gcgcgccgcg ttacggatgt aatcgcggcg
     4921 gcggtatgac gcactggcgg atccgtgcgc ctgacgtcga cgcaggacgg attcgattcg
     4981 aacgggtccc ggtagcgcgc ctcggaggcg cgtagtgcct tctcgacctg caggcgcgcg
     5041 gcggtgtcgc ggccgactgc ctggattccg atcaggtacc cgcggcatcg agcagcggga
     5101 cgtcgctccg cgaccgccag cgctccgagc catcggctgc cgttacgcgg tgctcgtagc
     5161 tccgctgcgg gactcgaggc ccaacggctt gcccgttccg cccgtccggc ggtagtcgat
     5221 cccgatcgct cttaccgcac agactgcagc gaatagatgt agtcg
//
LOCUS       DBNHBY010000012         2795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_474253, whole genome shotgun sequence.
ACCESSION   DBNHBY010000012 DBNHBY010000000
VERSION     DBNHBY010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_474253"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC16HB_00350"
     CDS             <1..913
                     /locus_tag="AC16HB_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5605267.1"
                     /translation="PAVPDRSRRDRASNLSFRMYRLSIHLTRLPIQRIGACRRVSMPT
                     ASHISPQRSPLFVAGLAKGLAALGAFSAERPAMSLAELAAATGLTRSSVQRLVFTLES
                     LGYLRRAHDSGRFELTPRTCELGFRYLETSRLVAASNPYLFDLNRRCGETVNLSEPDG
                     ADMMFVARFPGHKSIAIHMPIGRRLPMYCTASGRAYLSKLPGKDVRDLLRSGERPAVT
                     STTVTAEHALHRLITEAAACGFAYANEEYYRGDINVAAALTRPGGAPAGAINISVPAS
                     RWTLAQAKKELAPLVIETARAISSKLA"
     gene            963..1415
                     /locus_tag="AC16HB_00355"
     CDS             963..1415
                     /locus_tag="AC16HB_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605268.1"
                     /translation="MNNPTRWTLAIVVTLLLVFAWADDFVTLQGEYTIYTVSCEDGQW
                     KSTLCSGRLAAGDRYRYRALRQRKEVLFWRVGVSEPSGKFTNCAITDGRNWTCKANED
                     ATRSITLGLVRGRAQHDTSGVTKTFHAISKWKWWCLRVGLGKFNRASY"
     gene            complement(1461..>2795)
                     /locus_tag="AC16HB_00360"
     CDS             complement(1461..>2795)
                     /locus_tag="AC16HB_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5605269.1"
                     /translation="KALAALERHEDALRDLEVALKLAPADASMHLQRGIALAALKRFD
                     EARASFEQALRLNPDLAAAHASLGAALFKLKRFEEAIDALDEALRRDPRLAVAYYNKA
                     ATLRELAQISAALECCNRAIDLEPGLADAHALKAHLKILRGEYEEGWELLEWRWKVQQ
                     KKAVRNFAQPLWLGHESIRDKVLLIHAEMGLGDTLHFCRYAALARDRGARVILEVQAP
                     LVSLLEASLDGASAVLAQGTALPDFDVHCPTMSLPHAFRTTVATIPARIPYLSAPESK
                     RVAWRQKIGASAAVRVGLTWFASALHDTGPAKSVPLAQMTRLFELPLEFHILQKDMPV
                     TDRPILEGFANVRIHSEDLEDFSDTAAIVDLMDLVVTVDTSVAHLAGAIGKETWILLP
                     YAADYRWMLDRSDSPWYPTATLIRQPRRGDWESVVSTVHRRLAGLNADARGR"
BASE COUNT          481 a          952 c          875 g          487 t
ORIGIN      
        1 gcctgctgtt ccggatcgtt cacggcggga tcgcgcatcg aatctctctt tccgtatgta
       61 tcgattatcg atacacttaa ctcgattgcc gatacagcgt atcggcgcat gccgccgcgt
      121 gtcaatgccc accgcctcgc acatctcgcc gcagcgctcg cccctcttcg tggccgggct
      181 cgcgaagggg ctcgccgcac tcggcgcctt cagcgccgaa cgtccggcga tgagcctggc
      241 cgaactggcg gccgccacgg gcctgacgcg cagttcggtg cagcgactcg tcttcacgct
      301 cgagtcgctc ggctacctgc gccgcgcgca cgattcgggc cgcttcgaac tcaccccgcg
      361 cacgtgcgaa ctcggctttc gctacctgga gacgagccgt ctcgtcgcgg ccagcaatcc
      421 gtacctgttc gacctcaacc ggcgctgcgg cgaaaccgtg aacctctccg aacccgatgg
      481 cgccgacatg atgttcgtgg cacggttccc cggccacaag agcattgcca tccacatgcc
      541 gattggccgc aggctgccca tgtattgcac cgcctctggt cgcgcgtacc tgtcgaagct
      601 gccgggcaag gacgtccgtg acctgttgcg ctccggcgag cggcccgcgg tcacgtccac
      661 cacggtgacg gccgaacacg cgctccaccg cctgatcacc gaggcggcag cgtgcggctt
      721 cgcctacgcc aacgaggagt actaccgcgg cgacatcaac gtcgccgccg cattgactcg
      781 cccgggcggc gcaccggccg gtgcgatcaa tatttccgtg cctgccagcc gctggacgct
      841 cgcgcaggcg aagaaggaac tggcgccgct cgtgatcgaa accgcacggg cgatttcatc
      901 gaagctggcg tgaacgggac gccgcgctac gatcggctcc gacaggacgc gatcgattgc
      961 acatgaacaa tccaacgcgc tggacgctgg cgatcgtggt gacgttgctg ctcgtcttcg
     1021 cctgggccga tgacttcgtg acgctgcagg gcgaatacac gatctacacg gttagctgcg
     1081 aggacgggca atggaagagc acgctctgct ccggtcggct ggccgccggc gaccgctacc
     1141 gctaccgcgc gctacggcag cgcaaggagg tgctgttctg gcgcgtcgga gtttccgagc
     1201 cgtcgggcaa gttcacgaac tgcgcgatca cggacggccg caactggacc tgcaaggcca
     1261 acgaggacgc aacccgttcg atcacactcg ggctggtccg cggccgcgcg cagcacgata
     1321 cgtccggcgt cacgaaaacg tttcacgcga tcagcaagtg gaagtggtgg tgcctgcgcg
     1381 tcgggctggg gaagttcaat cgcgcgagct attgatcacg gtggataccg gacgaccgta
     1441 gcgtcgaata ctgaccgtca tcagcgcccg cgcgcatccg cattcagccc ggccagccgt
     1501 cgatggacgg tcgagacgac gctctcccag tctcctctcc gcggctgtcg gatcagggtc
     1561 gcggtcggat accacggaga gtcggaccgg tcgagcatcc agcgatagtc ggcggcgtac
     1621 ggcagcagga tccacgtctc cttgccgatc gcgcccgcca ggtgcgcgac cgacgtgtcc
     1681 acggtcacga ccagatccat cagatcgacg atggccgccg tatcggagaa atcttccagg
     1741 tcctcgctgt ggatacgcac gttcgcaaaa ccctcgagga tcgggcgatc cgtcacaggc
     1801 atgtcctttt gaagaatgtg gaactcgagc ggcaattcga acagccgtgt catctgcgcc
     1861 agcggcacgc tcttggccgg gccggtgtcg tgcagtgccg aagcgaacca ggtcagccct
     1921 acgcggacgg cggcgcttgc gccgatcttc tgccgccacg ccacgcgctt cgactcgggc
     1981 gccgacagat acggtatgcg cgcgggaatc gtcgccaccg tcgttcgaaa tgcgtgcggc
     2041 aagctcatcg tcgggcagtg gacatcgaaa tcgggcagtg ccgtgccctg cgcaagcacc
     2101 gcactcgcac cgtccaggct ggcctcgagc agagacacca gcggcgcctg gacttcgaga
     2161 atcacccggg cgccgcgatc ccgcgccagc gccgcgtagc gacagaagtg cagcgtgtcg
     2221 cccaggccca tttcggcgtg aatcaggagc accttgtcgc gaatcgactc atggccgagc
     2281 caaagcggct gcgcgaagtt gcgtacggct ttcttctgtt gaaccttcca gcgccattcg
     2341 agaagttccc agccttcttc gtactctccg cggagaatct tcaggtgcgc cttcaacgca
     2401 tgggcgtcgg ccagtccggg ctccaggtcg atggcccgat tgcagcactc gagggctgcg
     2461 ctgatctgcg ccagttctcg cagcgtcgcg gccttgttgt agtacgcaac ggcaagacgc
     2521 ggatcgcgcc gcagcgcctc gtccagagcg tcgatcgctt cctcgaagcg tttcaatttg
     2581 aatagggccg cgcctagtga cgcgtgagcc gccgcgaggt cggggttcag tcgcagcgct
     2641 tgctcgaaac tcgcgcgcgc ctcgtcgaat cgtttcaacg ccgccagagc gatcccgcgt
     2701 tgcaggtgca tcgacgcatc ggccggagcc agcttcagcg cgacttcgag atcgcgcaag
     2761 gcgtcttcgt gcctttccag cgccgccagc gcctt
//
LOCUS       DBNHBY010000013         5266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1624965, whole genome shotgun sequence.
ACCESSION   DBNHBY010000013 DBNHBY010000000
VERSION     DBNHBY010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1624965"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16HB_00365"
     CDS             <1..315
                     /locus_tag="AC16HB_00365"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5605270.1"
                     /translation="YFRFCDEVVAAGCTAPIVPGIMPITNFTQLARFSDNCGAEIPRW
                     VRLKLASFGDDKASIRAFGLEVVTDLCERLLAAGAPGLHFYTMNQADAALALWQRLGL
                     PK"
     gene            complement(322..984)
                     /locus_tag="AC16HB_00370"
     CDS             complement(322..984)
                     /locus_tag="AC16HB_00370"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5605271.1"
                     /translation="MKLVIGNKNYSSWSLRPWLLLRHAGIAFEEVKLSFNSPTFKRDA
                     LAFGPTGKVPVLVDDGVTVWDTLAIAEYVAEKFPDRQLWPRDARQRAEARAICAEMHS
                     GFAALRTHMSMNVTANLAGLGWNIAVQDDIDRIGAIWSGLRARHKASGPFLFGAFSIP
                     DAYYAPVVTRFNTYKPKLAPALDEYMQTMLALPALKEWFGAAAQENEFVPQDESYRRP
                     QN"
     gene            complement(1010..1696)
                     /locus_tag="AC16HB_00375"
     CDS             complement(1010..1696)
                     /locus_tag="AC16HB_00375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5605272.1"
                     /translation="MDGEIRTRPRAADRTRLLPLTLYGAKGSGVAAIEAALRLIGIGY
                     RLVDAASWQPGPGLEELQRVNPLAQVPTLILDDGAVMTESSAILIELGLRHPGSGLLP
                     AAEARRAQAIRGLTFIAANCYAAIGVVDYPERWCAPCDDELAKRICAGTRTRLHQLWD
                     VFADTFPAAPFLSGERIGALDLLAAVVSRWSGARAHLKQSRPRFHALLQTIECVPSVA
                     PVFEAHWPTQ"
     gene            complement(1699..2136)
                     /locus_tag="AC16HB_00380"
     CDS             complement(1699..2136)
                     /locus_tag="AC16HB_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HIR5605273.1"
                     /translation="MMGEWGPVTSRTIDLGHEQLLMLEARRGTRMKVIYGGVWLTEEG
                     EPQDLFALGGDEVALRARRRSVIEGLGATRVELIEPVAGGRVRALVRGAAAALRRLIP
                     VAAWSPHVPRGTLAALAAVVGVAIPALVAVGIAATASTLARFV"
     gene            2256..3227
                     /locus_tag="AC16HB_00385"
     CDS             2256..3227
                     /locus_tag="AC16HB_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5605274.1"
                     /translation="MASRPAPARAVPRRRRPIQLASLRGFEAAARLLSFTLAADELAL
                     TQSSISRQIGALERQVGKPLFLRKTRALELTFAGERLQRAVQQALTAIDRSVDEIRGV
                     GLPPRITLTTYASFASLWLVPRLAVFQREHPEIEIRIDAADRFIDLRKEGVDVALRWC
                     NPERTPVPGDAIELLQEYVTPAVSSRLIERTRVTLNAPADLYKLPLLELDDSTPSSVA
                     SSWQRWFEFAGVPPQAPAGGRMTFTFIDQAVQAAVRGQGAVMGRSPLIEDSVATGDLT
                     MPFPQLRMQTGYRYYLVVNPERAHLAHVTAFSQWLLTQFQQGPQRLT"
     gene            complement(3240..3764)
                     /locus_tag="AC16HB_00390"
     CDS             complement(3240..3764)
                     /locus_tag="AC16HB_00390"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR5605275.1"
                     /translation="MRARLLAARMNMEDREERERVLVSRVSRWLHTMPVKRLAFFWPT
                     RGEPNLAQLITSWLAEDSTREAGLPIVVGDLLEFAPWTPSTPMEAGTYGIQVPASKRR
                     MKPQLLLIPCLGIDEKRYRLGYGAGYYDRTLAYLSPRPVAVGIGFDCARIKTINPKPH
                     DIRLDLALTEAGSL"
     gene            3846..>5266
                     /locus_tag="AC16HB_00395"
     CDS             3846..>5266
                     /locus_tag="AC16HB_00395"
                     /inference="COORDINATES: protein motif:HMM:NF026068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5605276.1"
                     /translation="MLLRRTAFLAAIAALGIAASPVDATGQARTAARTTVLSVDDAFM
                     AAREAFRVGDRERLAMLADRIKGHPLEAYVEYWQLAARLRSDEPARVDAAVADFFARH
                     PGTFVADRLRLDWLLALGARRDFDAFAREQEALVWGDDAQIRCYSMLARYLREGRNDA
                     LARDARQLLAGTRESGTEGCVALTDALLEDGRVSVWERMRALVEQNQFATAKRMAAGA
                     KGTTPALIAQAIDQPAAFLATHERRLTEVQRELALVAVARLARDDPPQAARFAAALNL
                     HLTPEQRGIVWGRIGHMAALKLAPEALDWYRRGGEHVGIGPDAIRADEVLEWQVRTAL
                     RGDRNGPDWRMARSAIERMPAALAREPAWVYWRARALQAEERDAEATELLRSIAGRFD
                     FYGKLAAEELGEPIVVPPRAAEPSADEIAAMAAKPGFARALAFYRLGLRVEGHREWNW
                     QLRGTTDRQLLAAAEYARSVNVL"
BASE COUNT          809 a         1874 c         1771 g          812 t
ORIGIN      
        1 tacttccgct tctgcgacga ggtcgtcgcg gcaggctgca cggcgccgat cgtgccgggc
       61 atcatgccga tcacgaattt cacccagctg gcgcgcttct ccgacaactg cggcgccgag
      121 atcccgcgct gggtccggct gaagctcgcg agcttcggtg acgacaaggc gtcgatccgc
      181 gcgttcgggc tcgaggtcgt caccgatctg tgcgaacggt tgctcgccgc cggcgcgccg
      241 gggctgcact tctacacgat gaatcaggcc gacgccgcgc tcgcgctatg gcagcggctc
      301 ggactgccga aatgagcctc gtcagttctg cggacggcga tacgactcgt cctgcggaac
      361 gaattcgttc tcctgcgcgg cggcgccgaa ccactccttc agcgccggca gcgcaagcat
      421 cgtctgcatg tactcgtcca gcgccggcgc gagcttcggc ttgtacgtgt tgaagcgcgt
      481 gaccaccggc gcgtagtacg cgtcggggat gctgaacgcg ccaaacagga acggcccgct
      541 cgccttgtgg cgcgcacgca gcccgctcca gatcgcgccg atgcggtcga tgtcgtcctg
      601 caccgcgatg ttccagccga gccccgccag gttcgccgtg acattcatgc tcatgtgcgt
      661 gcgcagcgca gcgaagccgg aatgcatctc ggcgcagatc gcgcgcgctt ctgcgcgctg
      721 gcgcgcgtcg cgcggccaca gttgccggtc cgggaacttc tcggccacgt actcggcgat
      781 cgccagcgtg tcccacaccg tcacgccgtc gtcgaccagc accggcacct tgccggtcgg
      841 gccgaacgcg agcgcgtcgc gcttgaacgt cggcgaattg aaactgagct tgacttcctc
      901 gaacgcgatg ccggcgtgcc gcaacagcag ccacggccgc agcgaccacg acgagtagtt
      961 cttgttgccg atcacgagct tcacgcatcc tcctcgacca gatcaaccct cattgcgtcg
     1021 gccagtgcgc ctcgaacacg ggcgccacgc tcggcacgca ctcgatcgtc tgcagcagcg
     1081 catgaaagcg cggccgactc tgcttcaggt gcgcgcgcgc gcccgaccac cgcgacacca
     1141 cggcggccag caggtcgagt gcgccgatcc gctcgccgct gaggaacggc gccgccggaa
     1201 acgtgtcggc aaagacatcc catagctggt gcagccgcgt gcgtgtcccg gcacagatac
     1261 gcttggccag ttcgtcgtca cacggcgcgc accagcgctc gggatagtcg accacgccga
     1321 tcgccgcgta gcagttggcg gcaatgaacg tcaggccccg gatcgcctgc gctcgacgcg
     1381 cttcggccgc tggcagcagg ccgctgccag gatggcgcag cccgagctcg atcaggatcg
     1441 cactcgactc ggtcatcacc gcgccgtcgt cgaggatgag cgtcggcacc tgcgccagcg
     1501 ggttcacgcg ctgcagttcc tccagcccgg gcccgggctg ccacgacgcc gcgtccacca
     1561 gccggtagcc gatgccgatc agtcgcagcg ccgcctcgat ggcggccacg ccggatccct
     1621 tcgcgccgta cagcgtcagc ggcaggagtc tcgtgcggtc ggccgctcgc ggccgcgtgc
     1681 gaatctcgcc atccatcgtc agacgaaccg cgcgagcgtc gaggccgtgg ccgcgatgcc
     1741 gaccgcgacc aacgcgggaa tcgccacgcc gaccacggcc gccagtgcgg cgagcgttcc
     1801 gcgtggcaca tgcggcgacc aggccgccac cggtatcagg cggcgcaacg cagccgccgc
     1861 accgcgcacc agcgcacgca cgcgcccgcc tgcaaccggc tcgatcagct cgacgcgggt
     1921 ggcgcccaat ccttcgatca ccgagcggcg gcgcgcgcgc agtgccactt cgtcgccgcc
     1981 gagcgcgaac aggtcctgcg gctcgccttc ctcggtcagc cagacaccgc cgtagatcac
     2041 cttcatgcgc gtaccgcgcc gggcctcgag catcagcaac tgctcgtggc cgagatcgat
     2101 cgtccgcgac gtgactggtc cccattcacc catcatgaca acctccactg cgtgattggc
     2161 cttgcggcac ggacgaatta gaggacagcg gcaccgcgcc gacaaacgaa aagagtgcaa
     2221 ttgatgcatg cgcaatttgc atgtatggtc gcgtcatggc ttcccgccct gcgcccgcgc
     2281 gtgccgtgcc gcgccgtcgt cgcccgatcc agctcgcatc gctgcgcggc ttcgaggccg
     2341 cggcgcgcct gctgtcgttc acgctcgcgg ccgacgagct ggcgctgacg cagtcgtcga
     2401 tcagccgcca gatcggcgcc ctcgagcgcc aggtgggaaa gccgctgttc ctgcgcaaga
     2461 cgcgggcact ggagctgacg ttcgccggcg aacgactgca gcgcgccgtg cagcaggcat
     2521 tgacggcaat cgaccgcagc gtcgacgaaa tccgcggcgt cggcctgccg ccgcggatca
     2581 cgttgaccac gtatgcgtca ttcgcatcgc tgtggctggt gccgcggctg gcggtgtttc
     2641 agcgcgagca ccccgagatc gagatccgca tcgacgccgc cgatcgtttc atcgacctgc
     2701 gcaaggaagg cgtggacgtc gcattgcgct ggtgcaaccc cgagcgcacg ccggtgccgg
     2761 gcgatgccat cgaactgctg caggagtacg tgacgccggc cgtcagcagc cggctgatcg
     2821 agcgcacgcg cgtgacgctg aatgccccgg ccgacctgta caagctgccg ctgctggaac
     2881 tggacgacag cacgccgagt tcggtcgcga gcagctggca gcgctggttc gaattcgccg
     2941 gcgtgccgcc acaggcgccc gccggcggcc gcatgacgtt cacgttcatc gaccaggcgg
     3001 tgcaggccgc ggtccgcggc cagggtgcgg tgatgggacg ctcgccgctg atcgaggatt
     3061 ccgttgccac cggcgacctg acgatgccgt ttccgcagct gagaatgcag accggctatc
     3121 gctactacct cgtcgtcaac ccggagcgcg cgcacctggc acacgtgacc gcgttttcac
     3181 aatggctgct gacacaattc cagcaggggc cgcagcgact gacctgagaa ccactgggct
     3241 cagagcgatc cggcctccgt cagggccagg tcgagccgga tatcgtgcgg cttcgggttg
     3301 atcgtcttga tgcgcgcgca gtcgaagccg atgccgaccg cgaccgggcg cggcgacagg
     3361 tacgccagcg tgcgatcgta gtaaccggcg ccgtagccga ggcgatatcg cttctcgtcg
     3421 atgcccaggc acggtatcag cagcagttgc ggcttcatgc gccgtttcga cgccggcacc
     3481 tggatgccgt acgtgccggc ctccatcggc gtcgacggcg tccacggcgc gaactcgagc
     3541 agatcgccga cgacgatcgg caatcccgct tcgcgcgtgc tgtcctcggc cagccaggac
     3601 gtgatcagtt gcgccaggtt cggttcgccg cgcgttggcc agaagaacgc cagccgcttc
     3661 accggcatgg tgtgcagcca gcgcgacacg cgactgacca gcacgcgttc gcgctcttca
     3721 cgatcctcca tgttcatgcg cgctgccagt agacgcgcgc gcatgccgcc ccgttcggga
     3781 tcaggagcct ttccgccgat cgtgtttcct gccatctgcg tgcgccgcgt gctaacgtga
     3841 cttcgatgtt attacgtcgt accgcctttc ttgcggcgat tgccgcgctg ggcatcgcgg
     3901 cctcgcctgt cgacgctacg ggacaagccc gcaccgcggc ccgcaccacc gtgctgtccg
     3961 tcgacgacgc gttcatggca gcgcgcgagg cgttccgcgt cggcgatcgc gagcggcttg
     4021 ccatgctggc cgatcgcatc aaaggccacc ctctcgaagc ctacgtcgaa tactggcagc
     4081 tcgcggcgcg gctgcgcagc gatgagccgg cgcgcgtcga cgccgcggtc gcggatttct
     4141 tcgcccgcca tcccggcaca ttcgtggcgg atcgcttgcg gctcgactgg ctgctggcgc
     4201 tcggcgcgcg gcgcgatttc gacgcgttcg cacgcgaaca ggaggcgctg gtctggggcg
     4261 acgacgcgca gatccggtgc tattcgatgc tggcgcgcta tctgcgcgaa ggtcgcaacg
     4321 acgcgctcgc gcgcgacgcg cgccagttgc tcgccggcac gcgcgagtcc ggtaccgaag
     4381 gttgcgtcgc gctgaccgac gcgctgctgg aggacggccg tgtgtcggtg tgggagcgca
     4441 tgcgcgcgct ggtcgagcag aaccagttcg caaccgccaa gcgcatggcg gccggggcca
     4501 agggaaccac gcctgcgctg atcgcgcagg cgatcgacca gccggccgcg ttcctggcca
     4561 cgcacgagcg acggctgacc gaagtgcagc gcgaactggc gctggtcgcg gtcgcgcgtc
     4621 tggcacgcga cgacccgccg caggcggcgc gcttcgccgc ggcgttgaac ctgcacctga
     4681 cgcccgagca gcgcggcatc gtgtggggcc gcattggcca catggcggcg ctgaagctcg
     4741 cgcccgaagc gctcgactgg tatcggcgcg gcggcgaaca tgtcggtatc ggtcccgacg
     4801 cgatacgcgc ggacgaggtg ctcgagtggc aagtgcgcac ggcgctgcgc ggcgatcgca
     4861 acggccccga ctggcgcatg gcgcgctcgg cgatcgaacg aatgccggcc gcgctggcgc
     4921 gcgagccggc ctgggtctac tggcgcgcgc gcgcgctgca ggcggaggag cgcgacgccg
     4981 aggcaaccga gttgctgcgc tcgattgccg gccgcttcga cttctacggc aagctcgccg
     5041 ccgaggaact cggcgagccg atcgtcgtgc cgccacgcgc cgccgagccg agcgcggatg
     5101 agatcgccgc gatggccgcc aagccgggct tcgcgcgcgc gctggcgttc tatcgcctcg
     5161 gtctgcgcgt cgagggacat cgcgaatgga actggcagct gcgcggcacg accgatcggc
     5221 agctgctggc cgcggccgag tacgcgcgca gtgtcaacgt gctcga
//
LOCUS       DBNHBY010000014         7051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1692407, whole genome shotgun sequence.
ACCESSION   DBNHBY010000014 DBNHBY010000000
VERSION     DBNHBY010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1692407"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC16HB_00400"
     CDS             complement(<1..812)
                     /locus_tag="AC16HB_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5605277.1"
                     /translation="MALVPPSITDDSGAGVIARFLRARGVDRVFGLCGGHIMPIWMRL
                     DAEGIRIIDVRDERAAVYMAHAHAELTGELGVAIITAGPGVTNAMTGIANAHVARAPV
                     LLLSGVPPRPQENRGALQDMTHTDFVKPLTRYARTVREPSLILQELDEAVARAFGQGG
                     DPGPVYLDFPVDTLRAQVPHAVQLAEHLAPKPRPRIYPDPAAVARAVDLLWSAPRVLV
                     ISGRGARGAGALVRLLDRLNALYLDTGESRGLVPENHPSVVAAMRGSVMGEA"
     gene            complement(1126..2220)
                     /locus_tag="AC16HB_00405"
     CDS             complement(1126..2220)
                     /locus_tag="AC16HB_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5605278.1"
                     /translation="MARFEHSRSNETVPQTERWKRRPADSTWGDFGPDDQLGRLNLLT
                     PEKVLQGIAEVRAGKTFCLSLPLDLPGGNLLNPRRFPPVLRPTLRNGRPNLNYRLMTD
                     DPLHTDVVNDDLAILYLQYSTQWDSLAHVGQMFDADDDGIPEAVYYNGFRPALHILGP
                     SDPADAGVPPRAADARSTSAARALGIENMAAHGLQGRGVMVDLAAHLGRARVVVGYDL
                     LMSILDQDRVVVEAGDMVCLHTGFAQRLIEMKGVPDREVLHNSCAALDGRDGRLLQWI
                     TDVGLAVLIADNYAVEVLPATSHDRCCATLPLHEHCLFKLGVNLGELWHLTPLAEWLR
                     ANDRCRFLLTAPPLRLPGAVGSPVTPIATV"
     gene            complement(2354..4684)
                     /locus_tag="AC16HB_00410"
     CDS             complement(2354..4684)
                     /locus_tag="AC16HB_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605279.1"
                     /translation="MSFAFGDVLSRSDVLSVRPLISDTTDPNIQHVADDLARLAADDP
                     ASVANVVREILSSALPDAHAEIASAITEGLSDQTLQSLAQTPAGRELLDVLDHRGSTG
                     AVVVDQQGQFDRLRDASQSVLQRSADWSWAGIAMHQGIRANALNSASANASTEGSDAA
                     TREQVKSAFKEEFAAKAANKQDFDAFMQQVYGDKYDKNLAEQFRQRALAGDFSWLPDV
                     KFVDAATLRGGNGAYNEKEGVVYINRDVAAADPSKAAQVFVEEAGAHLDAKLNKVDTQ
                     GDEGEMFRRVLSGEQLSAREIAAIRADDDHGTITVDGKQVEVEFWFGEDIVDGVGDVA
                     SSVVDKTKEAVSDVVDYAGNAARDVVYSVGDTIKETGMGVINGLETFMQGMAVDFFAG
                     TFMNLLQGHIADAFDSMTRGIDKMVFQAPRRIFNGALEGVGHWLKTATYLLPAKLGGD
                     FMRDVIDRGIDSVRSVANGAIDIVRNFVMLPLHIIGGVERDIGEALKYFARGDIDKGF
                     GRLGLAFVHPFERVAGMVVDDVSIAAQAAGNVFGNMAGIVIDTQPPSRGLSKNERDWL
                     KSVYGESLNLEDIRIHRSDLGTDAGMAPHTVGNDIYLPDNCFNADGTLNEEGRMTLVH
                     EAFHAYQAQHEGNEYIHGALEAQVGGIVGSGNRNAGYNWLPALQSGKPFNEWNPEQQA
                     KFIEDMARARSQRLDTDRDANRTLDGSYDANGNGQIDRAELEMVLGDIGGQTGIARNG
                     RIDGDETRVSMTDDDFQRAMAIWNTLRDDRPDRALV"
     gene            5566..6807
                     /locus_tag="AC16HB_00415"
     CDS             5566..6807
                     /locus_tag="AC16HB_00415"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006623 - protein targeting to vacuole
                     [Evidence IEA];
                     GO_process: GO:0031929 - TOR signaling [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA];
                     GO_process: GO:0071444 - cellular response to pheromone
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5605280.1"
                     /translation="MAKLLVTLQGVALREVGLSRKMTTIGRRSRNCLVMDMLGVSGEH
                     AVVRHCGADFVIEDLGSTNGTLVNGQPVTKQVLRTGDSIEIGEYRLLFIADDGEDSTG
                     DPDRERPRSLPGSSSEEVRDAIPIPPACAVEDTSAYDRPRLRILSGAKAGREYRLRKV
                     LTRLGRLGRETAAITRRADGYFIEHHRGEVFPKVNGVVIGSNARRLADHDVIEVAGVT
                     IEFVSDRVSVAVGRRSQLKSEPESPSAGSEAIELPATPSVATPKGASASGDSRASDRL
                     GTAQDQRLPMHTSSRASLPLPQFATDRLRNLLNIRTRRAVPKRGPRPNPGARISFEDL
                     RMTMQAGMTADLWRWLQDAGWREITYRPDRRHYCDIPMECVSELIECGAEERRDVLVS
                     CIEDARKGRQPLSTEFPVSNR"
     gene            complement(6872..>7051)
                     /locus_tag="AC16HB_00420"
     CDS             complement(6872..>7051)
                     /locus_tag="AC16HB_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605281.1"
                     /translation="SALGAVLVGLGVILFLAYNRQAIHKVIKLAPEVGVLPEAQSVWR
                     GRMASQAFNATGVVD"
BASE COUNT         1174 a         2315 c         2301 g         1261 t
ORIGIN      
        1 tcggcctcgc ccatcaccga accgcgcatc gcagccacga ccgacgggtg attctcgggc
       61 acgagaccgc ggctttcacc ggtgtccaga tacagcgcgt tgagccggtc gagcagccgc
      121 acgagcgctc ccgcaccacg ggcaccgcgg ccgctgatca ccagcacgcg cggggccgac
      181 cacagaaggt cgactgcacg cgccacggcc gccgggtcgg gatagatccg cggtctgggc
      241 ttgggcgcaa gatgctcggc aagctgaacc gcgtgcggga cctgggcccg cagcgtgtcc
      301 accgggaaat cgaggtagac cggacccggg tcccctccct ggccgaacgc acgcgcgacc
      361 gcctcgtcga gttcctgcag gatcaggctc ggctcgcgca ccgtgcgcgc atagcgcgtc
      421 agcggtttca cgaaatccgt gtgcgtcatg tcctgcaacg cgccgcgatt ctcctgtggc
      481 cgcggcggca ccccggagag cagcagcacc ggcgcgcgcg cgacgtgcgc gttcgcgatg
      541 ccggtcatcg cgttggtgac gcccggcccc gcggtgatga ttgcgacacc gagttcgccg
      601 gtcagctccg cgtgcgcatg cgccatgtag acggcagcgc gctcgtcccg aacgtcgatg
      661 atgcggatac cttcggcgtc aagccgcatc cagatgggca tgatgtgtcc gccgcacagt
      721 ccgaagacgc ggtcgacacc gcgcgcccgc agaaaacggg cgatcacgcc ggcgccggaa
      781 tcgtcggtga tcgatggagg aacgagtgcc atggttctaa cccttgcgtc gactgcaact
      841 cgcgttgcga caagcgaagc gggtgcggca tggcctggcg atgctgcccc ggcgccggaa
      901 tcggaccggg gatcatgatc tgcgtcgacc agcccggcaa cccgtcgctt gggcatggcc
      961 tcgaagctct gctgtgccgc gtgcacagcg gcttcgggtc caatgattga cggcagtcag
     1021 tcacgcggca catcgaaagc gagatgccgc accactgcag cacacggctt ggcgccacac
     1081 cagcaaacag cgcggaacgg tgcagaagaa atcgcgccgg tcgggctaca ccgtcgcgat
     1141 cggcgtcacc ggcgagccga ctgcgcctgg aaggcgcagc gggggtgcgg tcagcaggaa
     1201 tcgacagcga tcgttcgcac gcagccactc tgctagcgga gtgagatgcc acagctcgcc
     1261 gaggttcacc ccgagcttga aaaggcaatg ctcgtgcagc ggcagcgtcg cgcaacagcg
     1321 gtcgtgagaa gtggccggga gcacctcgac cgcatagttg tcggcaatca gcaccgcgag
     1381 gccgacgtcg gtgatccact gtagcagtcg accgtcgcgt ccatcgagcg cggcgcagga
     1441 gttgtgcagg acttcgcggt ctggaacgcc cttcatttcg atcagacgct gtgcgaagcc
     1501 cgtgtgcagg cacaccatgt cgccagcctc gacgaccacg cggtcctggt ccaggatcga
     1561 catcagcaag tcatatccga ccacgactcg cgcgcgaccc agatgcgcgg cgagatcgac
     1621 catcacgccg cgaccctgca gaccgtgcgc ggccatgttc tcgatgccga gggcgcgcgc
     1681 cgcgctggtt gagcgggcgt cagccgctcg tggcggaacg ccggcatcgg ctgggtcgct
     1741 cgggccaagg atgtggagag cgggcctgaa cccgttgtag tagacggctt cgggtatgcc
     1801 atcgtcatcg gcgtcgaaca tctgcccgac atgcgccagg ctgtcccact gcgtcgaata
     1861 ctgcagatac aggatggcca ggtcgtcgtt gacgacgtcc gtatgcagcg ggtcgtcggt
     1921 catgagccgg taattcaggt tgggccggcc attgcgcagc gtcggccgca gcaccggcgg
     1981 gaagcgccgc ggattgagca agttgccgcc cggtagatca agtggcaggc tgaggcaaaa
     2041 ggtcttgccg gcgcgaactt cggcaatgcc ttgcaggact ttctccgggg tcagcaggtt
     2101 gaggcgcccg agctgatcat ccggcccgaa gtcgccccac gtggaatcgg caggcctgcg
     2161 cttccagcgt tcagtctgcg gcaccgtttc gtttgaacgt gaatgttcaa atctggccat
     2221 ggcgcgcctt ccggagactc ggtcacggtc atcctacagc gtgatccgag tcgacagcgt
     2281 cgagccggcg ctcccggatg tcttcaatga gctggagtcg gatgcgcgcc aggccaagag
     2341 gctcgtcggc tgatcacacc agcgcgcgat ccggacggtc gtcgcgcagc gtgttccaga
     2401 tcgccatcgc gcgctggaaa tcgtcgtcgg tcatactgac cctggtctcg tcgccgtcga
     2461 tgcggccatt gcgggcaatg ccggtctgtc ctccgatgtc ccccaggacc atctccagct
     2521 ccgcgcggtc gatctggccg ttgccgtttg cgtcgtagct gccgtccagc gtccggttgg
     2581 cgtcgcggtc cgtgtccagc cgttgcgacc tcgcccgcgc catgtcctcg atgaacttgg
     2641 cttgctgctc cgggttccac tcgttgaacg gtttgccgga ctgcagcgcc ggcagccagt
     2701 tataacccgc gttgcggttc ccgctgccga cgatgccgcc tacttgcgcc tcgagcgcgc
     2761 cgtggatgta ctcgttgcct tcgtgctgcg cctgataggc gtggaacgcc tcgtgcacca
     2821 gcgtcatccg gccttcctcg ttgagcgtgc cgtcggcgtt gaaacagttg tccggcaggt
     2881 agatgtcgtt gccgaccgtg tgcggcgcca tgccggcatc ggtgccgagg tcgctgcggt
     2941 ggatgcggat gtcctcgagg ttcagcgatt cgccgtagac gctcttcaac cagtcgcgct
     3001 cgttcttgct caggccgcgc gacggcggct gagtgtcgat cacgatgccg gccatgttgc
     3061 cgaacacgtt gcccgccgcc tgcgcggcga tgctgacgtc gtcgaccacc atgccggcga
     3121 cgcgctcgaa cggatgcacg aacgccagcc cgaggcgccc gaaacccttg tcgatgtcgc
     3181 cgcgcgcgaa gtacttcagc gcctcgccga tgtcgcgctc gacgccgccg atgatgtgca
     3241 gcggcagcat cacgaagttg cgcacgatgt cgatcgcgcc gttggcaacg ctgcgcacgc
     3301 tgtcgatgcc gcggtcgatc acgtcgcgca tgaagtcgcc gccgagcttc gcgggcagca
     3361 ggtaggtggc ggtcttcagc cagtgaccga cgccttcgag cgcgccattg aagatccggc
     3421 gcggtgcctg gaacaccatc ttgtcgatgc cgcgcgtcat cgagtcgaac gcgtcggcaa
     3481 tgtggccctg gagcaggttc atgaatgtgc cggcgaagaa gtcgaccgcc atgccttgca
     3541 tgaacgtctc caggccgttg atcacgccca tcccggtttc cttgatcgtg tcgccgacgg
     3601 agtagacgac gtcgcgcgcg gcgttgccgg cgtaatcgac gacgtcgctg accgcttcct
     3661 tggtcttgtc gacgacgctg ctcgccacat cgccgacccc gtcgacgatg tcttcgccga
     3721 accagaactc gacttcgacc tgcttgccgt cgacggtgat cgtgccgtgg tcgtcgtcgg
     3781 cgcggatcgc tgcgatctcg cgtgcggata gctgttcgcc gctcaggacg cggcggaaca
     3841 tctcgccctc gtcgccctgg gtgtcgacct tgttgagctt cgcgtccagg tgcgcgcctg
     3901 cctcttcgac gaacacctgc gcggccttgc tcggatcggc cgcggccacg tcccggttga
     3961 tgtagacgac gccttccttc tcgttgtagg cgccgttgcc gccgcgcagc gtcgcggcgt
     4021 cgacgaactt cacgtcgggc agccaggaga agtcgccggc cagcgcgcgc tgccggaatt
     4081 gctctgcgag gttcttgtcg tacttgtcgc cgtacacctg ctgcatgaaa gcatcgaagt
     4141 cctgcttgtt cgcggccttg gcggcgaatt cctccttgaa tgcacttttc acttgctccc
     4201 tggtcgcagc atcgctgcct tccgtgctgg cgtttgcgga cgcactgttc agcgcattgg
     4261 cccgaatgcc ttggtgcatg gcgatgcctg cccaggacca atccgcggaa cgctgcaaaa
     4321 cggattgact ggcgtcgcgg agccggtcga actgcccttg ctggtcgacc accaccgcgc
     4381 ccgttgagcc gcggtgatcg agcacatcca ggagttcgcg tcccgcgggt gtctgagcca
     4441 ggctctgcag cgtctgatca ctcagaccct cggtaatggc gctggcgatc tccgcatgcg
     4501 cgtcgggcag cgccgacgac aggatctcac gcacgacgtt cgcgacactt gccgggtcat
     4561 ccgcagccag acgggccagg tcgtccgcca cgtgctgaat gttcggatcg gttgtgtcgg
     4621 atatcagcgg gcggacggat agcacatcgc tgcgtgacaa tacgtcgccg aatgcgaaag
     4681 acatgagcgg ctcccctgtg gaccgcggcg taagttgcgg ctatccgcgc tttggttcag
     4741 gtctcttatc ggactgagtt cgcgatcgcg ggaggacctt ttcagtggcc gtccgcggca
     4801 tctcagcctc ggttatgcgg cgcaccgacc gcgcccgctt gtagaccgcc atctttcagc
     4861 agcatcaccc gagcacctgt gcgtctgatc cgccggcgcg gcggtaaaga cctggtcgag
     4921 ccgcgccgcg caccgaagtt gcgaaccaac gacaagcgtc acgcccagcc gcacgaacac
     4981 cgccgagccc gagtaagcga ctgaacattt ccgcgtagtg acgggcggtg gtcagcgcgg
     5041 acacgaagca gtcggcccca accagccgcg tccatctgcc ctgcggcggg gtgcaagggg
     5101 aatttcgcgt cgatgtcagg gccggcgact gcgtctacca cgtggttccg cagctcgacg
     5161 atgctgcaag cgctcgatcg gtgcacgcgc aagaacctgg tccaagcact gggcgatcga
     5221 ggatgcggtc ggtcggagcc tgtcggaact ctttacatcg ttgatggcgc gccgtcgcgc
     5281 aagtcaagaa aatcaacgag ttggcgtgcc ggtatggtcc ttgctgcagg tcggcttagt
     5341 cattgttcgg ccctgcatgg gaggaaagcg accgggtcaa ccgtgtttcc ttctgctttg
     5401 gtttgctgac agaaacgctg ctcttcctgc tcaccccggg gtgcgcggcg actttgacga
     5461 acgcctacca ccgatgcgcg ctgtcaagca tcctgggttc gacagcacga actgaaacat
     5521 gactccgatc gctctgccgt ctcggcccga aaggacgaag cgacgatggc gaaactgctt
     5581 gtgacgcttc agggcgtcgc gctgcgcgag gtcggtttgt cgcgaaagat gaccacgatc
     5641 ggccggcgca gccgcaattg cctggtcatg gacatgctcg gcgtcagcgg agaacatgcc
     5701 gtagtgcgcc actgcggtgc agactttgtc attgaagacc tgggttccac caacggaacg
     5761 ctcgtcaatg gccagccggt gacgaagcag gtcttgcgga ccggcgacag cattgaaatc
     5821 ggcgagtacc ggctgctgtt cattgcagac gacggcgaag attcgacggg cgaccctgat
     5881 cgcgaaagac cgcggtccct gccgggcagt tcctcggagg aggtgcggga cgcgatcccg
     5941 atcccgccgg cgtgcgcagt cgaggacact tctgcgtacg accggcctcg cttgcgcatc
     6001 ctgtccggcg cgaaggcggg ccgggaatat cggctccgca aggtgctgac tcggcttggg
     6061 cgccttggcc gcgagaccgc agccatcacg cgccgtgcgg acggctactt catcgaacac
     6121 catcgcggcg aagtcttccc gaaggtcaat ggagtcgtga tcggctcgaa cgcacgtcga
     6181 ctcgcggatc acgatgtcat cgaggtcgcg ggcgtcacga tcgagtttgt gagtgaccgg
     6241 gtgtcggttg cggtcggtcg acgctcacaa ttgaagtccg aaccagaatc gccctctgcg
     6301 ggctctgaag cgatcgagtt gccggcaacg ccgagtgtcg cgacgccgaa gggcgccagc
     6361 gcaagcgggg attcccgcgc gtctgatcga ctcgggactg cacaggatca gcgattgccg
     6421 atgcacacga gctcaagggc gagtctgcct ctcccccagt tcgcgaccga ccggctgcgc
     6481 aacctgctca acatccgcac gcgccgagcg gtacccaagc gtggtcctcg tccgaatccc
     6541 ggggcgcgca tctccttcga ggatctgcgc atgacgatgc aggccggaat gaccgccgac
     6601 ctgtggcgct ggctgcagga cgcggggtgg cgcgagatca cttatcggcc cgatcggcgc
     6661 cattattgcg acatcccgat ggagtgcgta tcggagctga tcgaatgcgg cgccgaggag
     6721 cgacgtgacg tcctggtctc gtgcatcgag gatgcgcgca agggtcgcca gccactttcg
     6781 acggagtttc cggtctcgaa ccgctgaatc ccggcgctcg tccctagccc ccgctccatg
     6841 ccggcgggcg cagttccgcg caccgctgca tttagtcgac gactccagtc gcattgaacg
     6901 cctgcgacgc catccgcccg cgccacacgc tctgcgcctc cggcaacacg cccacctccg
     6961 gcgcgagctt gatgaccttg tgaatcgcct gccggttgta ggcgaggaac aggattacgc
     7021 cgaggccgac cagcacggcg ccgagggcgg a
//
LOCUS       DBNHBY010000015         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_948306, whole genome shotgun sequence.
ACCESSION   DBNHBY010000015 DBNHBY010000000
VERSION     DBNHBY010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_948306"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="AC16HB_00425"
     CDS             complement(<1..625)
                     /locus_tag="AC16HB_00425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5605282.1"
                     /translation="MKILVPVKRVVDANVKVRVKSDGSDVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVATEVVAVSCGATQCQETLRTALAIGADRAILVETSEELQPLSVAKLLQ
                     ALVGKEQPQLVILGKQAIDDDCNQTGQMLAALLDWGQATFASKVEIADGKARVTREVD
                     GGVETLGLALPAVVTADLRLNEPRYVTLPNIMKAKKKPLQTVKPADLG"
     gene            complement(655..1845)
                     /locus_tag="AC16HB_00430"
     CDS             complement(655..1845)
                     /locus_tag="AC16HB_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR5605283.1"
                     /translation="MSRANPRTSFHWDDPLLIDQQLTQDERQVREAARAYCADKLQPR
                     VLEDFRHGKTDPAIFREMGELGLLGPTIPEAYGGAGLNYVSYGVITREVERVDSGYRS
                     MMSVQSSLVMVPINEFGTEAQKQKYLPKLASGQWIGCFGLTEPNHGSDPSSMVTRARK
                     VPGGYSLSGAKMWISNSPIADVFLVWAKDDEGAIRGFVLEKGWKGLSAPEIHGKVGLR
                     ASITGEIVMDGVFCPAENAFPEVRGLKGPLTCLNSARFGIAWGALGAAEDCFHRARQY
                     VLDRKQFGRPLAANQLIQKKLADMLTDISLGLQGCLRLGRMKDEGNAAVETTSIMKRN
                     SCGKALDIARVARDMMGGNGISDEFGVARHLVNLEAVNTYEGTHDIHALILGRAITGI
                     QAFN"
     gene            complement(1922..>2116)
                     /locus_tag="AC16HB_00435"
     CDS             complement(1922..>2116)
                     /locus_tag="AC16HB_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5605284.1"
                     /translation="CAKLFCTEMVGRVADRGVQVHGGAGYINEYPVERFYRDVRLLRL
                     YEGTTQIQQLIIGRELMGRG"
BASE COUNT          332 a          693 c          670 g          421 t
ORIGIN      
        1 cgcccaggtc ggccggcttg acggtttgca gcggcttctt cttggccttc atgatgttgg
       61 gcaaggtgac atagcgcggc tcgttcagcc tcagatcagc ggtaaccacc gccggcagcg
      121 ccaggccgag agtctcaacg ccgccgtcga cctcgcgcgt gacccgcgcc ttgccgtcgg
      181 cgatctccac tttggaggcg aaggtggcct gaccccagtc gagcagcgcc gcgagcatct
      241 ggccggtctg gttgcagtcg tcgtcgatgg cttgtttgcc gaggatgacg agctggggct
      301 gctctttgcc gaccagcgct tgcagcaact tcgccaccga cagcggctgc aattcttcgc
      361 tggtctcgac caggatggcg cggtccgcgc cgatggccaa cgcggtgcgc agcgtctcct
      421 ggcattgcgt cgcgccgcag gaaacggcca cgacctccgt ggccactccc ttctccttga
      481 gccgcaccgc ttcttcgacg gcgatctcgt cgaacgggtt catgctcatc ttgacgttgg
      541 caatatccac gtccgagccg tcgctcttga cgcgcacctt cacgttggcg tccaccacgc
      601 gcttgacggg tacgagaatc ttcatcggca tctcccttaa cgaagccacg cgggtcaatt
      661 gaaggcctgg atgcccgtga tcgcgcgccc cagaatcagc gcatggatgt catgcgtacc
      721 ttcgtaggta ttgacggcct ccaggttgac caggtggcgc gccacgccga actcgtcgct
      781 gatgccgttg ccgcccatca tgtcgcgggc aacgcgcgcg atgtccagcg ccttgccgca
      841 ggagttgcgt ttcatgatcg acgtggtctc caccgcggca ttgccttcat ccttcatgcg
      901 gcccagccgc aggcagcctt gcagtccgag actgatgtcg gtgagcatgt cggccagctt
      961 cttctggatc agctgattgg cggccagggg gcgcccgaat tgcttgcggt cgagcacgta
     1021 ttggcgcgcg cggtgaaagc aatcctcggc tgcaccgagc gcgccccagg cgatgccaaa
     1081 gcgcgcggag ttcaggcagg tgagcgggcc cttcaagccg cgcacctcgg ggaaggcgtt
     1141 ctccgccggg cagaacacgc cgtccatcac gatctcgccg gtgatgctgg cgcgcagacc
     1201 gaccttgccg tgaatctccg gcgcggacag acccttccat cccttctcga gcacgaagcc
     1261 gcgaatcgcg ccttcgtcgt ctttggccca cacgagaaag acgtcggcga tcgggctgtt
     1321 ggagatccac atcttggcgc cgctcaagct gtagccgccc ggcaccttgc gggcgcgtgt
     1381 gaccatgctg ctggggtcgg aaccgtggtt gggctcggtc agaccgaagc agccgatcca
     1441 ctgtccggac gccagcttcg gcaggtattt ctgcttctgc gcttccgtgc cgaactcgtt
     1501 gatcggcacc atgacgagcg acgactgcac gctcatcatt gagcggtagc cggagtcgac
     1561 ccgttcgact tcgcgcgtga tcacgccata agagacgtag ttgaggccgg cgccaccgta
     1621 ggcttccgga atggtcggcc ccagcagtcc gagttccccc atctcgcgaa agatcgcggg
     1681 atcggtcttg ccatgccgga agtcctccag cacgcgcggc tgcagcttgt cggcgcagta
     1741 ggccctggct gcctcgcgca cctggcgttc gtcttgcgtg agctgctggt cgatcagcag
     1801 cggatcgtcc cagtggaaac tggtgcgggg gttggctcgg ctcatgtcga tctcctgtcg
     1861 cggagggcgt tcgttggggt gtcgagggtg tgcctcttgg caattggcgc aacttcggct
     1921 gtcagccgcg acccatcagt tcgcggccga tgatcaattg ctgaatctgc gtggtgcctt
     1981 cgtatagacg cagcaggcgc acgtcgcgat agaagcgctc gaccgggtat tcgttgatgt
     2041 agcccgctcc gccgtgcacc tgtacgccgc ggtcggcgac ccgaccgacc atctcggtgc
     2101 agaacaactt ggcgca
//
LOCUS       DBNHBY010000016         2547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1286494, whole genome shotgun sequence.
ACCESSION   DBNHBY010000016 DBNHBY010000000
VERSION     DBNHBY010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1286494"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="dnaQ"
                     /locus_tag="AC16HB_00440"
     CDS             complement(<1..512)
                     /gene="dnaQ"
                     /locus_tag="AC16HB_00440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR5605285.1"
                     /translation="MNRQVVLDTETTGLEAKEGHRIIEVGCLELVGRRLTERRLHHYV
                     NPERDSDEGALAVHGLSTEFLADKPKFADIASELIEFVRGTEVIIHNAAFDLEFLDAE
                     LARLSLGRFEDYCRVTDSLKLARELHPGKRNSLDALCERYAVSNAHRTLHGALLDAGL
                     LADVYLAMTRG"
     gene            complement(515..>2547)
                     /locus_tag="AC16HB_00445"
     CDS             complement(515..>2547)
                     /locus_tag="AC16HB_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605286.1"
                     /translation="RATLIPLVTIPLSLVGAFALMYAFGFTINTLTLLAMVLAIGLVV
                     DDAIVVLENIYRNIEHGMGRAAAAMKGIKEIGFAVMAMTLTLTAVFAPLAFATGRTGR
                     LFIEFALALAGAVLVSGFVALTLSPMMCSKLLQHEAKHGRAFRTIEGWIDALTRGYAR
                     SLAWVLERRWLIVAVWFVTAGIAALVFMQLRSELAPLEDRGVIFGRLQSPPGSTVDYT
                     SEQLRAVERYYDTIPERAAYNAVAGFPTVDFGNAILRLKPWEERTRKQQDIARELNAK
                     FQQLPGVIGFAVNPPSLGQSPRQLPVEYVIMSQVPYAELDRIVTQFIAEIAKTGIVQS
                     PQSDLRLNTPELRVEVNRDKLADLGIPVENVGRTLQTMLGGRQVTRFKKDGEQYDVIV
                     QVAPRDRSRPNDISDIYVRGRDGAMVQLANVVRVQEGVSPQSLNHFQRLRAVTISATL
                     APGYALSDALNMMDATAKRVLPQTVVTDLNGVSREFRDARGSIYLTFVLALVFIYLVL
                     AAQFESFADPFVIMLSVPLSLAGALLALWLAGGTLNIYSQIGLITLVGLITKHGILIV
                     EFANQLQERGERLFDAIRQAAVLRLRPILMTTGAMVLGAVPLAFSHGAGAESRIQIGW
                     VIVGGLTFGTLLTLYVVPTMYTIMEQVLGKWLGSHHHHELPGTEASQTRAAE"
BASE COUNT          422 a          876 c          845 g          404 t
ORIGIN      
        1 ccgcgcgtca tcgccaggta cacgtcggcc agcagtccgg cgtcgagcag cgcgccgtgc
       61 agcgtgcgat gggcgttgga caccgcgtag cgctcgcaca gcgcgtcgag cgagttgcgc
      121 ttgccggggt gcaactcgcg cgcgagcttc agcgaatcgg tgacgcggca gtagtcctcg
      181 aagcggccga ggctcagccg cgccagctcg gcgtcgagga actccaggtc gaacgcggcg
      241 ttgtggatga tcacctcggt gccgcgcacg aactcgatca gttcggacgc gatgtcggcg
      301 aacttcggct tgtcggcgag gaactcggtc gacaggccgt gcaccgcgag cgcaccctcg
      361 tcgctgtcgc gctccgggtt gacgtagtgg tgcaaccggc gctcggtgag ccggcggccg
      421 accagttcga ggcagccgac ctcgatgatg cggtgacctt ccttcgcttc gagtcctgtt
      481 gtctcggtgt cgaggacgac ctggcgattc atcgctactc ggccgcgcgc gtctggctgg
      541 cctcggtgcc gggcagctcg tgatggtggt gcgaaccgag ccacttgccg agcacctgtt
      601 ccatgatcgt gtacatcgtc ggcaccacgt acagcgtcag cagggtcccg aacgtgagtc
      661 cgccgacgat cacccagccg atctggatcc ggctctcggc gccggcgccg tgcgaaaacg
      721 ccagcggcac cgcgcccagc accatcgcgc cggtcgtcat caggatgggg cgcagccgca
      781 gcaccgccgc ctggcggatt gcgtcgaaga gccgctcgcc gcgctcctgc agctgattgg
      841 cgaactcgac gatcaggatg ccatgcttgg tgatcagccc caccagcgtg atcaatccga
      901 tctggctgta gatgttcagc gtcccgccgg ccagccacaa cgcgagcagc gcgcccgcca
      961 gcgacagcgg cacagacagc atgatcacga aggggtcggc aaagctttcg aactgcgccg
     1021 ccagcacgag gtagatgaag accagcgcca gcacgaaagt cagatagatg ctgccgcgcg
     1081 cgtcgcggaa ttcgcgcgac acgccgttga ggtccgtcac caccgtctgc ggcagcacgc
     1141 gcttggcggt ggcgtccatc atgttcagcg catcactgag tgcgtagccc ggcgcgagcg
     1201 tggcggaaat ggtcaccgcg cgcagccgct ggaagtggtt gagcgactgc ggcgacacgc
     1261 cctcctgcac gcgcaccacg ttggcaagct gcaccatcgc accgtcgcgc ccgcgcacgt
     1321 agatgtcgct gatgtcgttg ggccggctgc ggtcgcgcgg tgcgacctgc acgatcacgt
     1381 cgtactgctc gccgtccttc ttgaagcgcg tgacctggcg gccgcccagc atcgtctgca
     1441 gcgtacggcc gacgttttcc accgggatgc cgaggtcggc gagcttgtcg cggttgacct
     1501 cgacgcgcag ctccggcgtg ttcagccgca ggtcgctctg cgggctctgc acgatgccgg
     1561 tcttcgcgat ctcggcgatg aactgcgtga cgatgcggtc cagttccgcg tacggaacct
     1621 gcgacatgat cacgtactcg accggcagct ggcgtggcga ctggccgagc gatggcgggt
     1681 tgaccgcgaa gccgatcacg cccggcagct gctggaactt ggcgttgagt tcccgtgcga
     1741 tgtcctgctg cttgcgcgtg cgctcctccc agggcttgag gcgcaggatc gcgttgccga
     1801 agtcgaccgt cgggaatccg gccaccgcgt tgtaggccgc ccgttccgga atcgtgtcgt
     1861 agtaacgctc gaccgcgcgc agctgttccg acgtgtagtc gacggtggag ccgggcggcg
     1921 actgcaggcg gccgaagatc acgccgcggt cctccagcgg cgccagctcc gagcgcagct
     1981 gcatgaacac cagtgccgcg atgccggcag tgacgaacca cacggcgacg atcagccagc
     2041 gccgctcgag cacccacgcc agcgaacgcg cgtagccgcg cgtgagcgca tcgatccagc
     2101 cttcgatcgt ccggaacgcg cggccgtgtt tcgcctcgtg ctgcagcagc ttgctgcaca
     2161 tcatcggcga cagcgtcagc gcaacgaacc ccgacaccag caccgcaccc gccagcgcga
     2221 gcgcgaactc gatgaacagg cgcccggtgc gaccggtcgc gaacgccagc ggcgcgaaca
     2281 ccgcagtcag cgtcagcgtc atcgccatca ccgcgaagcc gatctccttg atccccttca
     2341 tggccgccgc ggcgcggccc atgccgtgct cgatgttgcg gtagatgttc tcgagcacca
     2401 cgatcgcatc gtcgaccacg aggccgatcg ccagcaccat cgccagcagc gtcagcgtgt
     2461 tgatcgtgaa gccgaacgcg tacatcagcg cgaacgcgcc caccagcgac agcgggatcg
     2521 tcaccagcgg gatcagcgtg gcgcgca
//
LOCUS       DBNHBY010000017         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_339386, whole genome shotgun sequence.
ACCESSION   DBNHBY010000017 DBNHBY010000000
VERSION     DBNHBY010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_339386"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16HB_00450"
     CDS             complement(<1..418)
                     /locus_tag="AC16HB_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5605287.1"
                     /translation="MKFGIGQAVTRREDDRLLTGQGRFVDDLSFPDELHLAFVRSPYP
                     YARITSIDAAEAQSIEGVAAVFTGAELMADGVGSFPGNPALKNAAGKPMSGPPLLPLA
                     TDTVRFVGQTVAAVAATSHAIAQRAAEAIAVDYEPLD"
     gene            complement(439..711)
                     /locus_tag="AC16HB_00455"
     CDS             complement(439..711)
                     /locus_tag="AC16HB_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR5605288.1"
                     /translation="MPRMIHCVKLGKEAEALDFPPVPGELGKKLWQSVSKEAWATWQK
                     HQTMLVNENRLNLADARARKYLLTQMEKYFFGDGADQPAGYVPPKE"
     gene            complement(726..>1682)
                     /gene="argA"
                     /locus_tag="AC16HB_00460"
     CDS             complement(726..>1682)
                     /gene="argA"
                     /locus_tag="AC16HB_00460"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR5605289.1"
                     /translation="MAHAQVRVISGNFVTARPLGILDGVDYQHTGLVRKVDADAIRFT
                     LGSNAIVLASPLGFSPTGEAFNLTMEDVASSIAIALKADKLIFVADIDGVRDRSGHLH
                     QELAMVQAETLLADDDVDSDAAYNLNFLLKACRGGVARAHIIPYALDGGVLIELFTHD
                     GVGTMISTEDIESMREATVEDVGGIIKLLEPLEAEGTLVKRPRELIEREVERFTVLEH
                     DGVIYGCAALYSFPDSKTGEMAAVTVHPAYQNYGDGERLMKRIEARARAAGLTRLFVL
                     TTRTSHWFLKRGFTLASVDELPIERQRLYNWQRRSQILIKTL"
BASE COUNT          272 a          552 c          548 g          310 t
ORIGIN      
        1 ggtcgagtgg ctcgtagtcg accgcgatcg cttccgccgc gcgctgtgcg atcgcatgcg
       61 aggtcgccgc aacggccgcc accgtctggc cgacgaagcg gaccgtatcg gtcgcgagcg
      121 gaagaagcgg cgggccgctc atcggtttgc cggccgcatt cttcagcgcc ggattgcccg
      181 ggaagctgcc gacgccatcg gccatgagtt ccgcacccgt gaacaccgcc gccacacctt
      241 cgatcgattg cgcctcggct gcatcgatcg acgtgatgcg cgcatacggg tagggcgagc
      301 ggacgaacgc gagatgcagt tcatccggaa acgaaagatc gtcgacgaac cggccctgac
      361 ccgtcagcag ccggtcgtct tcgcggcggg tgaccgcctg gccgatgccg aatttcatgg
      421 tgtctcgtgc cggctcgatc attccttcgg cggcacgtac cccgccggct ggtcggcacc
      481 atcgccgaag aagtacttct ccatctgcgt cagcaggtat ttgcgcgcgc gcgcatccgc
      541 gagattgagg cgattctcgt tgaccagcat cgtctggtgc ttctgccagg tcgcccacgc
      601 ctccttcgac acgctctgcc agagcttctt gccgagttcg cccggaacgg gagggaagtc
      661 gagcgcctcg gcttccttgc cgagtttcac gcagtggatc attcgaggca tgagctgctc
      721 cgctgtcaaa gggtctttat cagtatctgg gaacgccgtt gccagttgta caggcgctgg
      781 cgctcgatcg gcagttcgtc gacgctggcg agcgtgaagc cgcgcttcag gaaccagtgg
      841 ctggtgcgcg tcgtcagcac gaagagccgc gtcagccccg ccgcgcgcgc gcgcgcttcg
      901 atgcgcttca tcagccgctc gccgtcgccg tagttctggt acgcaggatg cacggtgacg
      961 gcggccatct cgcccgtttt cgaatcggga aacgaataaa gcgccgcgca gccgtagatc
     1021 acgccgtcgt gctcgagcac cgtgaagcgc tcgacttcgc gttcgatcag ctcgcgcggg
     1081 cgcttgacca gcgtgccttc cgcttcgagc ggctcgagca gcttgatgat cccgccgaca
     1141 tcctcgacgg ttgcctcgcg catcgactcg atgtcctcgg tcgagatcat cgtgcccacg
     1201 ccgtcgtgcg tgaacagctc gatcagcacg ccgccgtcga gcgcgtacgg gatgatgtgc
     1261 gcgcgtgcca cgccgccgcg gcacgccttg agcaggaagt tcaggttgta cgcggcatcg
     1321 gagtcgacgt cgtcgtcggc cagcagcgtt tcggcctgca ccatggcgag ctcctggtgc
     1381 aggtggccgg aacggtcgcg cacgccgtcg atatcggcga cgaagatcag cttgtcggcc
     1441 ttcagcgcga tggcaatgct gctggcaaca tcttccatcg tgaggttgaa cgcctcgccg
     1501 gtcggcgaga agccgagcgg ggaagcgagc acgatcgcat tgctgcccag agtgaaacgg
     1561 attgcatcgg cgtcgacctt gcgcaccaat ccggtgtgct ggtagtcgac gccgtcgagg
     1621 atgcccagcg gccgcgcggt gacgaagttg ccgctgatca cgcgcacctg cgcatgcgcc
     1681 at
//
LOCUS       DBNHBY010000018        11668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_880729, whole genome shotgun sequence.
ACCESSION   DBNHBY010000018 DBNHBY010000000
VERSION     DBNHBY010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_880729"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC16HB_00465"
     CDS             <1..523
                     /locus_tag="AC16HB_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5605290.1"
                     /translation="LGLPAARRGRLACPRTRIETRFPFGLWQAWAYYTPPLTAIVYPN
                     PEDDAPPLPVAAGGEGEGVGLASTGDDFAGVRPYQPGDPQKMIAWRLAARSDELSVKL
                     FDAQGGGDLVLDIDALPRQMPYEDRLARLARWVLMADAAHMQFGLQLHGRKIDLGSGA
                     EHRERCLIALALA"
     gene            528..2636
                     /locus_tag="AC16HB_00470"
     CDS             528..2636
                     /locus_tag="AC16HB_00470"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR5605291.1"
                     /translation="MSATAASMSGLNAAWQALRRLALSLVQPQVSSAARERRDVLLLM
                     LAIAFVVVPHFEHLTWWAIATFVALWAWRFWLTLAQRPAPGHLAMLPLLAAAAVAVWI
                     EHRTLLGRDAGVTFLLLLIALKLLELRARRDIFVVIFLAFFILLTQFLFDQGLPVALL
                     TFAAVALLFFVLLSVNLTDGDLPAARKLALVGVIVLKALPLTVALFVLFPRLSQPLWG
                     LPGDAYSGTTGLSNSMAPGSISRLLESNQIAFRVKFEGALPPKESLYWRGPVFGQFTG
                     RVWGPLQRRLAPSPPLATQADPASAVEYTMTLEPSQRDWLFALDLPLIPPPHDELNPR
                     LNGDGQLVAGTLVTQRVRYRLRSYTRYVMGLNETDPSLRDWVALPAGFNPRTLQWASE
                     LRRRTPNGQVRTGDPALVNAVLEHFRRGGYAYTLAPPLLGRHSIDEFLFDTKLGYCEH
                     YAAAFVVLMRALDIPARVVTGYQGGEINPVDGFMIVRQSDAHAWAEVWLEERGWVRVD
                     PTAVVAPVRIEQGAVEIARQAGIDLGINAATAQAPGWMRWARDARFRWEALQNAWNQW
                     VLAYSPERQRALLRYLGLQPDWQMLGLLLAITVVATLSVLAYLSLRYRSMRDPLAQLF
                     DRFRQRLLDAGVRVDFSLGPRALQAQLASSLAPPARRDAAAILAALERWRYSRAGDNV
                     SRAQLRSLRRAVRRFRPIPA"
     gene            2646..3683
                     /gene="mltB"
                     /locus_tag="AC16HB_00475"
     CDS             2646..3683
                     /gene="mltB"
                     /locus_tag="AC16HB_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR5605292.1"
                     /translation="MRRRAFLQRLAALSALAVPTGPAQAAFVPYPRRDEVRSFIDDVA
                     ARSDLKRAWIERVFAHGRYLPAVERLMQPPIPFGSRNWLEYRSRYVDERRIRDGVAFL
                     TLQRAALQRAAARYGVPVEIVTAILGVETRYGRMTGDFRTLDVLLTLSFDYLRRADYF
                     RSELEHFLLLSREQRLDPLAVRGSFAGAIGMPQFMPSSIRRWAVDFDNDGRIDLARSA
                     ADSIGSVANFLLDHGWQTDAPILLPATLGEDAGETTLEGLVEELGGGIRADTPWSMLA
                     GRGVEVDAALPLDAPVLLIDLPIVEADGTQRREYRLGTANFASILHYNRSYFYATSVV
                     ELAAALRAAAA"
     gene            complement(3684..4325)
                     /gene="maiA"
                     /locus_tag="AC16HB_00480"
     CDS             complement(3684..4325)
                     /gene="maiA"
                     /locus_tag="AC16HB_00480"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR5605293.1"
                     /translation="MKLFNAARSSASFRARIALHLKGLPFEYVPVGLVKGDQFKDDFR
                     AMNPAELVPVLQLDDGTTLTQSLAIIEYLDEKYPQPPLLPHDPVERAYVRAIALAIAC
                     EIHPLNNLRVLRYLVRTLGASDDAKNAWYRHWVEQGLAQVEAQLVAGRRCGRFVLGDR
                     VTLADVLIVPQIFNAQRYDCSLGHVPAIMRIFDACMQLPAFIAAQPEKQPDFA"
     gene            complement(4334..5653)
                     /locus_tag="AC16HB_00485"
     CDS             complement(4334..5653)
                     /locus_tag="AC16HB_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5605294.1"
                     /translation="MSGVEFGFLMFGVLVVLLLVRVPIGAAMFLVGVAGYVTLTGWAP
                     LLSYLKTQAYGRFAVYDLSVIPLFLLMGNFATHGGLSRALFSAASALIGHWRGGVAMA
                     AIGACAGFGAICGSSLATAATMGQVALPELRRLRYSSRLATGTLAAGGTLGILIPPPV
                     VLVVYAILAQQNIGKLFAAAFVPGILAALGYIAAIAIYVRIFPDAGPAGPRHALGEQL
                     RVLRDVWPVATIFLVVVGGIYGGVFTPTEAASVGTIGAGGVAFARGMRGREFVQCLLS
                     TAEATAMIFFILLGADMLNVFLALSQMPVELAAWVTGLGVAPLAVLFVILIVYVILGC
                     LMDSLSMILLTIPIFLPIILGLDYFGLDATEKSIWFGVLALMVVEIGLITPPVGMNVY
                     IINSMARDVPMAETFRGVAPFLLSDAVRVTLLVFFPILSLWLVRLID"
     gene            complement(5802..6323)
                     /locus_tag="AC16HB_00490"
     CDS             complement(5802..6323)
                     /locus_tag="AC16HB_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5605295.1"
                     /translation="MEAPAQPHGDAAGPVGRALNWLAQAFALAGGAVLFGMTLMSTVS
                     IVGRWLFGQPIRGDYEIAQLATAVAVSAFLPWCALRGGHVLVDFLTNGAPPRFRHALD
                     AIGQLSIAVVGLLVAWRLVDGTASLRDSGETTMVLGIPTWYAYAPMIPSFLLLGVVAL
                     HRAAAHARSALAQ"
     gene            complement(6345..7412)
                     /locus_tag="AC16HB_00495"
     CDS             complement(6345..7412)
                     /locus_tag="AC16HB_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5605296.1"
                     /translation="MKLRHALAALTLGWLCAGSGAAQEVVLKVHHFWPPGAMGPTNHL
                     VPWCEKIAQESNHRLKCQVFPAMQLGGTPPQLIDQVRDGVADIVWTLPGYTAGRFPIM
                     EVFELPFMINSAEAASRAAWDYYEKYAVKEFAGIKPLAFHVHDQGYVHTRDRQIRTLA
                     DFKGLKMRAPTRQTNKMLAAFGATPVSMPLPAVTEAMSKGVIDGFVLPWEVIPSVKLQ
                     EMVKYHTETDPSMPALYTAVFIFAMNPKKYDSLPPDLKAVIDRNSGPELSRQIGALWD
                     RSKPPARKTAVDRGNTFYTVPATELSQWERATRVLYADWISDMNKRGLNGNEMLSDAR
                     ALIAKYEKPGAKPEAQKAPRK"
     gene            complement(7457..8152)
                     /locus_tag="AC16HB_00500"
     CDS             complement(7457..8152)
                     /locus_tag="AC16HB_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5605297.1"
                     /translation="MGYVFAAPAQPVVPVAGSTDLFPVHRIYCVGRNYEEHAKEMGFS
                     GREPPFFFAKPADALLPIAEGSIGEMPYPSETSNLHHEFELVVAIGKRGRYIAAAAAP
                     GYVWGYAVGLDMTRRDLQGEAKKLGRPWEVGKGFDFSAPIGPIKPIAQTGAIKSGRIW
                     LNVNGEKRQASDVSKLIWNVAETIEHLSKYFELAPGDLIFTGTPEGVAAVKPGDLLEG
                     GVDGVGTLMVRIR"
     gene            complement(8175..9872)
                     /locus_tag="AC16HB_00505"
     CDS             complement(8175..9872)
                     /locus_tag="AC16HB_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605298.1"
                     /translation="MSAEISSSSSRPARDPAAFSLALALICAALVALPVLGVFASFFQ
                     APESADTLAHLIGTVIPRAALETFLLVLLVTAGVTFLGTTSAWLVAALEFPGRRVFEW
                     AMLLPLAMPGYIVAYAFTDFLQFSGPVQSALRDAFGWRRGDYWFPEIRTLPGAAFVFT
                     VVLYPYVYLLARTAFLARTASMIDAARSLGLSPLSTWLRVNLPLARPAVAAGALLALM
                     ETIADYGAAAYFGLQTFTTSIYRAWFSLGDRMAASQLAAVLLLVVLMITALELRARGR
                     ARFFAAPNSARPAPRTQLAGWRRWLAFVGCCVPITLGFVLPVAILIHLLLPALATAPF
                     GRYAQWLANSLLIGVLAAVLTLAVALALAYAVRLMQSGFVGRVVRLAARTLNLGYAVP
                     GAVMAVGILLPLAAFDNALDAWLRQTFGVSSGLLITGTLAALLYAYLVRYFAVGYQPV
                     EAGLARITPAMDASARSLGATPVEVFRRVHLPLLAPSVLAGCLLVFVDVMKELPATLV
                     LRPFNFDTLAVIAYQLAADERLGEAALPSLTIVAVGVAPVILLSRAIARASVVEAIG"
     gene            9978..11081
                     /locus_tag="AC16HB_00510"
     CDS             9978..11081
                     /locus_tag="AC16HB_00510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605299.1"
                     /translation="MTHASVIEIRDLSVGYRRGAPIVDGLTLALTRGEIGCVVGSSGC
                     GKTTLLRAIAGFIAVERGSIAIDGAVVAGTGLSLAPERRGVGLVFQDYALFPHLKVEQ
                     NVAFGLRGGDATVRNARVKTMLDLVGLGASANRYPHELSGGQQQRVALARALAPAPKL
                     LLMDEPFSNLDVELRARLGAEVRQILKDSTTSAILVTHDQQEAFAIADRVGVMHEGRL
                     EQWDRPYELYHRPASRYVADFIGQGVFLPAKVLNPHRVAIELGELSGDVPAPCGIGCD
                     VCGRGCRVEVLLRPDDVVHDDASSMTAQVVRKIFRGGDFVYTLKLDSGREVLALVPSH
                     HDHAIGERIGIRLDADHVITFPAAETTAQQRPL"
     gene            11100..11190
                     /locus_tag="AC16HB_00515"
     tRNA            11100..11190
                     /locus_tag="AC16HB_00515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11134..11136,aa:Ser,seq:gga)
     gene            complement(11240..>11668)
                     /locus_tag="AC16HB_00520"
     CDS             complement(11240..>11668)
                     /locus_tag="AC16HB_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5605300.1"
                     /translation="VAILAATVSAQAPADILFLVSAAFSLGASAFFIPLVAGVFWKRA
                     TKWGAIAAMVGGVGTTWYYMSTTQPWLRSVFGVTGSIADHTWFGISPISAGIFGMPVA
                     LVLMIVISYLTPAPDKQTQELVEHVRYPNLTGDTLDTRGT"
BASE COUNT         1969 a         4051 c         3898 g         1750 t
ORIGIN      
        1 gcttggactg cccgcggcgc ggcgcggcag gcttgcgtgt ccgcgcacac gcatcgagac
       61 gcgctttccg ttcgggctgt ggcaggcatg ggcgtactac acgccgccgc tcaccgcgat
      121 cgtgtacccg aacccggagg acgacgcgcc gccgctgccg gtcgccgcgg gcggcgaagg
      181 cgaaggcgtc ggccttgcgt cgacgggcga cgacttcgca ggcgtgcgcc cttaccagcc
      241 cggcgatccg cagaagatga tcgcgtggcg gctggccgcg cgatccgacg aactgtcggt
      301 aaagctcttc gacgcacagg gcggcggcga cctcgtgctc gacatcgatg cgctgccgcg
      361 acagatgcca tacgaggatc ggctggcgcg gctggcgcgc tgggtgctga tggccgacgc
      421 cgcacacatg cagttcggcc tgcagttgca cggccgcaag atcgatctcg gcagcggcgc
      481 ggagcatcgc gaacgctgcc tgatcgcgct cgcactggcg tgagcggatg agcgcgaccg
      541 ctgcgtcgat gtccggactt aatgccgcgt ggcaggcgct gcgtcggctc gccctgtcgc
      601 tggtgcagcc gcaggtatcg agcgcggcgc gcgagcgccg cgatgtgctc ctgctcatgc
      661 tggcgatcgc gttcgtggtg gtgccacact tcgagcacct cacatggtgg gcgatcgcaa
      721 ccttcgtggc gctgtgggcg tggcgtttct ggctgacgct ggcgcagcgg ccggcaccgg
      781 gacacctggc gatgctgccg ctcctcgcag ccgccgccgt cgcggtctgg atcgagcacc
      841 gcacgctcct cggacgcgac gccggcgtca cctttctgct gctgctgatc gcgctgaagc
      901 tgctcgaact gcgcgcgcgg cgcgacatat tcgtggtgat cttcctcgcg ttcttcatcc
      961 tgctgacgca gttcctgttc gaccagggac tgccggtcgc gctgctcacc ttcgccgcgg
     1021 tggcattgct gttcttcgtt ctcctcagcg tgaacctcac cgacggcgac ctgccggcag
     1081 cgcgcaagct cgcgcttgtc ggcgtgatcg tgctcaaggc gctgccgctg acggtggctc
     1141 tgttcgtgct gttcccgcgg ctgtcccaac cgctgtgggg gctgccgggc gacgcctact
     1201 cgggcacgac tggtctgtcg aattcgatgg cacccggctc gatcagccgg ctgctggaat
     1261 cgaaccagat cgcgtttcgc gtcaagttcg agggcgccct gccccccaag gagtcgctgt
     1321 actggcgcgg gccggtgttc ggccagttca ccggccgcgt gtggggcccg ctgcagcggc
     1381 gcctcgcacc gtcgccgccg ctcgcgacgc aggccgatcc ggcctcggca gtcgaataca
     1441 cgatgacgct ggagccgagc cagcgcgact ggctgttcgc gctcgacctg ccgctgattc
     1501 ccccgccgca cgacgagttg aacccgcgcc tgaacggcga cgggcaactg gtcgccggca
     1561 cgctcgtcac gcagcgggtg cgctaccggc tccgttcgta cacccgctac gtgatgggac
     1621 tgaacgaaac ggacccgtcg ctgcgcgact gggtcgcgct gccggcggga ttcaatccgc
     1681 gcacactgca atgggccagc gaactgcgcc ggcgcacgcc gaacgggcag gtgcgcaccg
     1741 gcgacccggc actcgtcaat gcggtactcg aacatttccg ccgcggcggt tacgcgtaca
     1801 cgctggcgcc accgctgctc ggtcgacatt cgatcgacga attcctgttc gacaccaagc
     1861 tgggctactg cgagcattac gcagccgcgt tcgtggtgct gatgcgcgca ctcgacatcc
     1921 ccgcgcgcgt ggtcacgggc taccagggcg gagaaatcaa tccggtcgac ggtttcatga
     1981 tcgtgcgaca gtccgatgcg cacgcgtggg ccgaggtgtg gctggaggaa cgcggctggg
     2041 tccgcgtcga tccgaccgcg gtggtcgcgc cggtccggat cgaacagggc gcggtcgaga
     2101 tcgcgcgcca ggccggcatc gacctcggaa tcaacgcggc gacagcccag gcgcccggat
     2161 ggatgcgctg ggcgcgcgac gcgcgcttcc gctgggaggc gctgcagaac gcttggaacc
     2221 aatgggtgct cgcgtactcg ccggagcgtc agcgcgcgct gctgcgctac ctcggactgc
     2281 agcccgactg gcagatgctg gggctgctgc tggcgatcac cgtcgtcgcg acgctgtcgg
     2341 tgctggcgta cctgtcgctg cgttatcgca gcatgcggga tccgctcgcg cagctattcg
     2401 atcgctttcg ccagcgcctg ctcgacgccg gcgtgcgggt cgacttcagc ctcgggccgc
     2461 gggcgctgca ggcgcagctc gcgagttcgc tcgcgccgcc ggcgcggcgc gatgcagcgg
     2521 cgatcctcgc cgcgctcgag cgctggcgct actcgcgcgc cggcgacaac gtgagccgcg
     2581 cgcagttgcg ctcgctgcgg cgcgcagtac ggcgttttcg gccgataccg gcttagcggg
     2641 aaaccatgcg gcgccgcgcc ttcctgcaac gtcttgccgc cttgagcgcg ctcgccgtgc
     2701 cgacagggcc ggcgcaggct gcattcgtcc cctacccgcg ccgcgacgaa gtgcggagct
     2761 tcatcgacga cgtggccgca cgcagcgacc tgaagcgcgc gtggatcgag cgcgtcttcg
     2821 cgcacgggcg ctacctgccc gcggtcgagc ggctgatgca gccgccgatt ccgttcggca
     2881 gccgcaactg gctcgaatac cgatcgcgct acgtcgatga gcgccgcatc cgcgacggcg
     2941 tggcgttcct cacactgcag cgcgccgcgc tgcaacgcgc ggcggcgcgc tacggcgtgc
     3001 cggtggagat cgtgaccgcg atcctcggcg tggagacgcg ctatgggcgc atgacgggtg
     3061 atttccgcac gctcgacgtt ctgctgacgc tgtcgttcga ctacctgcgc cgcgccgact
     3121 acttccgctc cgaactcgag catttcctgc tgctgtcgcg cgagcagcgg ctcgacccgc
     3181 tcgcggtacg cggctcgttc gcgggcgcga tcggcatgcc gcagttcatg ccgagctcga
     3241 tccgcaggtg ggcggtcgac ttcgacaacg atggtcgcat cgacctggcg cgcagcgcgg
     3301 ccgacagcat cggctccgtg gccaacttcc tgctcgacca tggctggcag accgatgcgc
     3361 cgatcctgct gccggccacg ctcggcgaag acgcgggcga aacgacgctc gaggggctgg
     3421 tcgaggaact gggcggcggc atccgcgccg acacgccgtg gtcgatgctt gccggccgcg
     3481 gcgtcgaggt cgacgcggct ttgccgctcg atgcacccgt gctgctgatc gacctgccga
     3541 tcgtcgaggc ggacggcacg cagcggcggg agtaccggct cggcaccgcg aacttcgcga
     3601 gcatcctgca ctacaaccgc agctacttct acgcgacctc ggtggtcgag ctcgccgccg
     3661 cgctgcgcgc agccgccgcg tagctacgcg aagtccggct gcttctccgg ctgcgccgcg
     3721 atgaacgccg gcagctgcat gcaggcgtcg aagatgcgca tgatcgccgg cacgtgaccg
     3781 aggctgcagt cgtagcgctg cgcgttgaag atctgcggca cgatcagcac gtccgccagc
     3841 gtcacgcgat cgccgagcac gaagcgcccg caccgccgcc cggcaacgag ctgcgcctcc
     3901 acctgcgcca gtccctgctc gacccagtgc cggtaccacg cgttcttggc atcgtcggac
     3961 gcgccgagcg tgcgcaccag gtagcgcagc acgcgcaggt tgttcaaggg atggatctcg
     4021 cacgcgatcg cgagcgcgat cgcacgcacg taggcgcgct cgaccggatc gtgcggcagc
     4081 agcggcggct gcgggtactt ctcgtcgagg tactcgatga tcgccagcga ctgcgtcagc
     4141 gtggtgccgt cgtcgagctg cagcaccggc accagctcgg ccggattcat cgcacggaag
     4201 tcgtccttga actggtcgcc cttgaccagc ccgaccggca cgtactcgaa cggcaggcct
     4261 ttgaggtgca gcgcgatccg cgcacgaaac gaggcagagg aacgggcagc gttgaagagt
     4321 ttcataagaa cctctagtcg atcagcctca ccagccacag cgaaagaatc ggaaagaaca
     4381 ccagcaacgt cacgcgcacc gcgtcggaga gcaggaacgg cgcgacgcca cgaaacgtct
     4441 cggccatcgg cacgtcgcgc gccatgctgt tgatgatgta gacgttcatg cccacgggcg
     4501 gcgtgatgag tccgatctcg accaccatca gggccagcac gccgaaccag atggacttct
     4561 ccgttgcatc gagcccgaag tagtccagcc cgaggatgat cggcaggaag atcgggatcg
     4621 tcagcaggat catcgacagg ctgtccatca ggcagccgag gatcacgtag acgatcagga
     4681 tgacaaacag caccgccaac ggcgccacgc cgagccccgt cacccaggcg gcaagctcga
     4741 ccggcatctg cgacagcgcg aggaacacat tcagcatgtc ggcgccgagc aggatgaaga
     4801 agatcattgc ggtggcctcg gccgtcgaca gcaggcactg cacgaattcg cgcccgcgca
     4861 tgccgcgtgc gaacgccacg ccgccggcgc cgatggtgcc gaccgacgcc gcctcggtcg
     4921 gcgtgaacac gccgccgtag atgccgccca cgacgacgag aaatatcgtg gccaccggcc
     4981 acacgtcgcg cagcacgcgc aactgctcgc cgagcgcatg gcgcggcccc gccggtccgg
     5041 cgtcggggaa gatgcgcacg tagatcgcga tcgccgcgat gtagccaagc gcggcgagga
     5101 tgccgggcac gaacgccgcg gcgaagagct tgccgatgtt ctgctgcgcc aggatcgcgt
     5161 acacgaccag caccactggc ggcgggatca ggatgccgag cgtgccgccg gccgcgagcg
     5221 ttccggtcgc caggcgcgac gaatagcgga gccgccgcaa ctccggcaac gcaacctgcc
     5281 ccatcgtcgc cgcggtagcg agcgacgagc cgcagatcgc gccgaagccg gcgcacgcgc
     5341 cgatcgcggc catggccacg ccgccgcgcc agtggccgat caacgccgat gccgcggaaa
     5401 agagcgcgcg cgacagccca ccgtgcgtcg caaagttgcc catcagcaga aagagcggaa
     5461 tcaccgacag gtcgtacacg gcgaagcggc catacgcctg ggtcttcaga tagctcagca
     5521 atggggccca gcccgtcagc gtcacgtagc ccgccacgcc gaccaggaac atcgcggcgc
     5581 cgatcggcac gcgcacgagc agcagcacga ccagcacgcc gaacatcagg aagccgaatt
     5641 cgacgccgct caagcgtccc ccttcggtcc agaggcgctt cccccaggag ggacgtgaga
     5701 accgtggctc caggcccgcc ggcgcggaat cggaccgccc ggacgcgcga cgtgtccggc
     5761 ggcaggccgc tcgagcgctc tcggggcagc ccggcccggt ctcattgcgc cagtgcgctt
     5821 cgggcgtgcg ctgccgcgcg atgcaatgcc accacgccca gcagcaggaa agacgggatc
     5881 atcggcgcgt acgcgtacca cgtcgggatg ccgagcacca tcgtcgtctc gccggagtca
     5941 cgcaggctcg ccgtgccgtc aacgagcctc catgcgacca gcagtccgac cacggcgatc
     6001 gacaactggc cgatcgcatc gagcgcatgg cggaaccgtg gcggcgcgcc gttcgtcaga
     6061 aagtcgacca gcacatggcc cccgcgcagc gcgcaccacg gcagaaaggc cgacacggcg
     6121 accgcggtgg ccagttgcgc aatctcgtag tcgccacgga tcggctgccc gaacagccag
     6181 cgcccgacga tcgacaccgt cgacatcagc gtcatgccga acagcaccgc gccgccggcg
     6241 agcgcgaacg cctgcgcgag ccagttcagc gcccgcccga ccgggccggc cgcgtcgccg
     6301 tgcggctgcg ccggcgcttc catctcgagc aacctgcgcg tgacctactt ccgcggtgcc
     6361 ttctgcgcct caggtttcgc gccgggcttc tcgtactttg cgatcagcgc gcgtgcatcg
     6421 gacagcattt cgttgccgtt cagcccgcgc ttgttcatgt cgctgatcca gtcggcgtac
     6481 agcacgcgcg tagcgcgttc ccattgcgac agctcggtcg ccggcaccgt gtagaaggtg
     6541 ttgccgcgat cgaccgcggt cttgcgcgcg ggcggcttgg agcggtccca cagcgcgccg
     6601 atctgtcgcg acaactcggg gccactgttg cggtcgatga ccgccttcag gtccggcggc
     6661 aggctgtcgt acttcttcgg attcatcgcg aagatgaaga ccgccgtata cagcgccggc
     6721 atcgacggat cggtctcggt gtggtacttg accatctcct gcagcttgac ggacggaatg
     6781 acttcccaag gcagcacgaa gccatcgatc acgcccttgg acatcgcctc ggtcaccgca
     6841 ggcagcggca tcgacaccgg cgtggcgccg aaggcggcga gcatcttgtt cgtctgccgc
     6901 gtcggggcgc gcatcttcaa gcccttgaag tcggcgagcg tcctgatctg ccggtcgcgc
     6961 gtatgcacgt agccctgatc atgcacgtgg aacgccagcg gcttgatgcc ggcaaactcc
     7021 ttgaccgcgt acttctcgta gtaatcccat gctgcgcggc tcgctgcttc cgcgctgttg
     7081 atcatgaacg gcagctcgaa cacctccatg atcggaaagc gaccggccgt gtagcccggc
     7141 agcgtccaca cgatatcggc cacaccatcg cgcacctgat cgatcagctg cggcggcgtg
     7201 ccgccgagct gcatcgcggg gaagacctgg cacttcaggc gatggttcga ttcctgcgcg
     7261 atcttttcgc accagggcac caggtgattg gtcggcccca ttgcacccgg cggccagaag
     7321 tggtgaacct tcagcaccac ttcctgcgcg gcgccgcttc cggcgcacag ccatcccaga
     7381 gtcagcgcgg caagcgcgtg acgcagcttc atcgttgcct cctcgttgtt gttgcgcgcg
     7441 gaattatcag cgtgtctcag cgaatccgca ccatcaacgt gccgacgccg tcgacaccgc
     7501 cctccagcag gtcgcccggc ttgaccgcgg ctaccccttc gggcgtgccg gtgaagatca
     7561 ggtcgccggg cgcgagttcg aagtacttcg acaggtgctc gatcgtctcg gcgacgttcc
     7621 agatcagctt gctcacgtcg ctcgcctggc gcttttcgcc attgacgttc agccagatgc
     7681 gtcctgactt gatcgcgccg gtctgcgcga tcggtttgat cggtccgatc ggcgcggaaa
     7741 agtcgaagcc cttgccgacc tcccacgggc ggccaagctt cttcgcctca ccctgcaggt
     7801 cgcgccgcgt catgtcgagc ccgacggcgt agccccagac gtaacccggc gccgcggcgg
     7861 ccgcaatgta ccggccgcgc ttgccgatcg cgacgacgag ttcgaattcg tggtgcaggt
     7921 tgctcgtctc gctcggatac ggcatttcgc cgatcgagcc ctcggcgatc gggagcagcg
     7981 cgtccgccgg tttggcgaag aagaacggcg gctcgcggcc ggagaagccc atctccttcg
     8041 cgtgttcctc gtagttgcgg ccgacgcagt agatccggtg taccgggaag agatcggtcg
     8101 agcccgcgac aggcacgacc ggctgcgcag gtgcggcgaa gacatagccc atggttcctg
     8161 accctctcgt tcgtctatcc gatcgcctcg acaaccgaag ccctggcgat ggcgcgcgac
     8221 agcaggatca ctggcgcgac accgacggcc acgatggtaa gtgatggcag cgctgcttcg
     8281 ccgagtcgct cgtcggcggc aagctgatac gcgatcaccg ccagcgtatc gaagttgaac
     8341 ggtcgcagca cgagtgtggc tggcagttcc ttcatcacgt cgacgaacac cagcaggcag
     8401 cccgccagca cggagggcgc gagcaacggc aggtgcacac gacggaagac ctcgaccggc
     8461 gtggccccga ggctgcgcgc actggcgtcc atcgccggcg tgatgcgcgc gagccccgcc
     8521 tcgaccggct ggtaaccgac cgcgaaatag cgcaccaggt acgcgtagag cagcgcggcc
     8581 aacgtgccgg tgatcagcaa cccgctgctg acgccgaagg tctgccgcag ccacgcgtcg
     8641 agcgcattgt cgaacgctgc cagcggcagc aggatgccga ccgccatcac cgcgcccggc
     8701 accgcgtagc cgaggttgag cgtgcgcgcc gccagccgca ccacgcggcc gacgaatcct
     8761 gactgcatca gccgcaccgc gtaggcgagt gcgagcgcaa cggcaagcgt cagcacggcg
     8821 gcgagcacgc cgatcagcag gctgttggcg agccactgcg cgtagcgacc aaagggcgcg
     8881 gtcgcaagcg ccggcagcag cagatggatc aggatcgcca ccggcaacac gaagccgagc
     8941 gtgatcggca cgcagcagcc cacgaacgca agccagcggc gccagccggc aagttgcgtg
     9001 cggggcgcgg gccgagcgct gttcggcgcg gcgaagaagc gcgcgcggcc gcgtgcgcgc
     9061 agttccagcg ccgtgatcat cagcacgacc agcagcaaaa cggcggcaag ctggctcgcc
     9121 gccatccggt cgccgagcga gaaccaggcg cggtagatcg acgtggtgaa cgtctgcaat
     9181 ccaaagtacg cggccgcgcc gtagtcggcg atcgtctcca tcagcgccag cagcgcgccg
     9241 gcggcgaccg cgggccgcgc cagcggcagg ttcacgcgca gccaggtcga aagcggcgac
     9301 agccccaggc tgcgcgccgc atcgatcatc gacgccgtgc gcgcgaggaa cgcggtgcgc
     9361 gccagcagat acacgtacgg atacagcacg accgtgaaca cgaatgccgc gcccggcagc
     9421 gtgcggatct cgggaaacca gtagtcgccg cgccgccagc cgaatgcgtc gcgcagcgcg
     9481 ctctgcacag ggcccgagaa ctgcaggaag tcggtgaacg cgtacgcgac gatgtagccc
     9541 ggcatggcga gcggcagcag catggcccac tcgaacacgc gccgacccgg aaattcgagc
     9601 gccgcgacca accacgccga ggtcgtccca agaaacgtca cgccggcggt caccagcagg
     9661 acgagcagaa aggtctcgag cgccgcgcgc ggaatgaccg tccctatcag gtgagcgagc
     9721 gtgtcggcgg actcgggcgc ctggaagaag ctcgcgaaca cgccgagcac cggcagcgcg
     9781 accaacgccg cgcatatgag cgccagcgcc agggagaacg cggccggatc gcgcgcggga
     9841 cgggaagaag aagacgagat ctctgcgctc accggattca ggtccgcgat cctgacacgc
     9901 gccgcgccga tggacgcaag atgttgcccg actcagatgc gaacaagaat tatatgcatc
     9961 tgtcgtaaca tcacgggatg acgcatgcca gcgtgatcga aatccgcgac ctttccgtcg
    10021 gttaccggcg cggcgcgccg atcgtcgacg ggctcacgct tgcgctcacg cgcggcgaga
    10081 tcgggtgcgt ggtcggcagc tccggctgcg gcaagaccac gctgctgcgt gcaatcgcgg
    10141 gtttcatcgc ggtcgagcgc ggttcgatcg caatcgacgg cgccgtcgtg gccggaacgg
    10201 gtctcagcct cgcgcccgag cggcgcggcg tcggactcgt gttccaggac tacgcgctgt
    10261 ttccacacct gaaggtcgag cagaacgtcg ctttcggcct gcgcggcggc gatgccacgg
    10321 tgcgcaacgc acgcgtgaag acgatgctcg acctcgtggg gctgggcgct tcggccaaca
    10381 ggtacccaca cgaactttcc ggcgggcagc agcaacgcgt tgcgctcgcg cgcgcgctgg
    10441 cgccggcacc caagctgctg ctgatggacg aaccgttttc caacctcgac gtcgaactgc
    10501 gcgcgcggct gggcgccgag gtgcggcaga tcctgaagga cagcaccacc tccgcgatcc
    10561 tcgtcacgca cgaccagcag gaagcgttcg cgatcgccga ccgcgtcggc gtgatgcacg
    10621 agggccggct cgagcaatgg gaccggccgt acgagctata ccaccggccc gccagccgct
    10681 atgtcgccga cttcatcggc cagggcgtct tcctgccggc caaggtgttg aacccgcacc
    10741 gtgtggcaat cgaactgggt gagttgtccg gcgacgtgcc ggcgccgtgc ggcatcggct
    10801 gcgacgtgtg cggcaggggc tgccgcgtcg aggtgctgct gcgtcccgac gacgtggtgc
    10861 acgacgacgc cagttccatg accgctcagg tggtgcgcaa gatcttccgc ggcggcgact
    10921 tcgtctacac gctgaagctc gacagcggcc gcgaagtgct tgcgctcgtt ccgagccatc
    10981 acgaccacgc gatcggcgag cgcatcggca tccgcctcga tgccgaccac gtgatcacct
    11041 tccctgccgc ggagacgacc gcgcaacagc ggcccctata atccgcgcgc tgcggcagcg
    11101 gagagatggc cgagcggttg aaggcgcacg cctggaacgc gtgtataggt gaatagccta
    11161 tcgtgggttc gaatcccact ctctccgcca aaacaaacgg ccccgcgagg ggccgtttgc
    11221 gttgaaccgc cagcgtcgat cacgtcccac gcgtatcgag cgtgtctccg gtcaggttcg
    11281 gataacgcac gtgctcgacc aattcctgtg tctgcttgtc gggcgccggc gtcaggtacg
    11341 agatcacgat catcagcacc agcgcgaccg gcatgccgaa gatcccggcc gagatcggcg
    11401 agattccaaa ccaggtatgg tccgcaatcg agccggtgac accaaacacg ctgcgcagcc
    11461 atggctgtgt ggtcgacatg tagtaccagg tcgttccaac accgccgacc atggccgcaa
    11521 tcgcgcccca tttcgtcgcg cgcttccaga acactccggc cacgagcggg atgaagaaag
    11581 ccgacgcgcc gagcgagaac gccgccgaga cgaggaacag gatgtcggcc ggggcctgcg
    11641 ccgacaccgt cgccgccagg atcgcgac
//
LOCUS       DBNHBY010000019         7948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_745552, whole genome shotgun sequence.
ACCESSION   DBNHBY010000019 DBNHBY010000000
VERSION     DBNHBY010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_745552"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16HB_00525"
     CDS             <1..462
                     /locus_tag="AC16HB_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HIR5605301.1"
                     /translation="LGVAVLIERRKDPSPWEPWRFRIADVMIDEGQFGNAARTLRDDG
                     RMTLTLHPGMSVTLFRDESEGYYLNLTSGAPVWFVMWRIDDGDPSRAWPEIVTLSYNE
                     AGRLLDAQERVDNLPLPAAIKDWLGAFTDEHYRPEPKQRRRPASFIAPSRR"
     gene            469..1116
                     /locus_tag="AC16HB_00530"
     CDS             469..1116
                     /locus_tag="AC16HB_00530"
                     /inference="COORDINATES: protein motif:HMM:NF023176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HIR5605302.1"
                     /translation="MTDHEKDEGFLSRWARRKAEARRGGVAPAESAPGNAAPAERTAR
                     ADERPPVAPTEPEGTPPAQDPPTLADVEVLTPQSDFTRFVSVGVDPEVKNAALKKMFA
                     DPHFNVMDGLDTSIDDYSRPDPIPADMLAKMTQSEHLELASRRLAAPPSPVDEVARRT
                     ASDVAPSAADDAPLPEPIAADENVDLQLQPHDAAGRAGAEPGAAEDIGREQRRAR"
     gene            1010..3223
                     /locus_tag="AC16HB_00535"
     CDS             1010..3223
                     /locus_tag="AC16HB_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5605303.1"
                     /translation="MKTLICNCNRTMPLDAPALNQALRKTSGASSDGLDKTHTLLCRR
                     EFGAFLQAAEETAATGDALVVACTQESRLFLDLDAESRGAAGRSQPIRFVNIRETGGW
                     SRDAASAMPKLAALIAAAQMPLPAPTTTVSYRSGGRCLVIGTADAVERAAAMLADRLD
                     VSLLVEGTGGAIEQRHDRAVYGGRLVRLSGWLGAFEAEWESRNPIDLELCTRCNACIA
                     ACPEHAIDFSYQIDLASCRSHRECVRACGAAGAIDFDRPSRATQERFDLVLDLRARPA
                     FVLHQPPQGYFHVGADERALVKAVLALRELAGEFEKPKFYSYQQNLCAHSRNEKIGCT
                     ACIDVCSARAIRSDAALKGKPRTAAPPRGPDAAPLLQPSGGGIVVEPHLCVGCGACST
                     VCPSGAMSFAYPALADQGRRLRTLLAAYAGAGGRDAALLLHSEGAGARLIDDLGRAAR
                     SDRSFHGVPARVLPLSVWHTASVGIELWLTAIAQGASNVWVLVTGEEAPEYRQALGEQ
                     MAVAQAILSGLGYEGTHFRLLEARDARDLPALDAALREPPARTVAVAAAYAAQANKRT
                     TLELAVDHLLAQAPVRADDIALPAAGAPFGAVQVNSETCTLCLSCVGACPASALADNP
                     ERPQLGFIEMNCVQCGLCASTCPERAITLQPRLWLADGGKARRATRILHQMEPYRCVR
                     CGKPFGTVRAIEKMLATLGAHSAFQGAAVERLKMCNDCRVIDMYTSEHEVRITEL"
     gene            3233..3859
                     /locus_tag="AC16HB_00540"
     CDS             3233..3859
                     /locus_tag="AC16HB_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR5605304.1"
                     /translation="MRTSTLQFNQPDDVEEISRADLYGLLSRLWLAAPDEALLARMRP
                     GTSGAGDAEGQLAAPWRALIAALRATDANAASVEYDALFQGVGKPEVMLYGSYYVTGF
                     LNEGPLVLLRSELRDLGLARDETRAETEDHIAFLFEVMRYLVAGDDAAVCNLERQRRF
                     FRAHLQPWVERLCDAVEAHPGARLWRAIAGLTRAFMQVEAQGFDMLEA"
     gene            3856..4479
                     /gene="mobA"
                     /locus_tag="AC16HB_00545"
     CDS             3856..4479
                     /gene="mobA"
                     /locus_tag="AC16HB_00545"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR5605305.1"
                     /translation="MSIRRDDITGLILAGGRGTRMGGVDKGLQNYRGMPLALHALLRL
                     APQVGQVMISANRNLAAYESMGVPVWPDPLPDYAGPLAGWLAGLERCTTPYLIAVPCD
                     TPAFPADLVERLSAGLAAADAEIAMAATRDAGAVRTQPVFSLMKSDLLESLSAFLRSG
                     QSKIMRWTGQRRCVEVVFDDASAFFNANTLDELQQLQEAHPDSARGT"
     gene            4486..5301
                     /locus_tag="AC16HB_00550"
     CDS             4486..5301
                     /locus_tag="AC16HB_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR5605306.1"
                     /translation="MNKTIKRYRLDGGPVRLKHFDPGARPYSTGGSREEDKARTAELA
                     HELASLQDNFYALRKERLLIVLQGMDTSGKDGTIRSVFQAIDPLGVRAVSYRAPTDPE
                     KQRDFLWRHHRDVPGVGEIVIFNRSHYEAVLIEYVHGWIDKDERKRRLKHINAFERLL
                     TDTGTTIVKCFLHISQDEQRRRLQERIDDPDKNWKFNLQDLEERKLWDDYQRAYEVAL
                     EETATTTAPWFVVPSDSKTHRNLMVTTILLDTLRRLAPKPPPAVKSLAGIKVR"
     gene            complement(5308..6006)
                     /locus_tag="AC16HB_00555"
     CDS             complement(5308..6006)
                     /locus_tag="AC16HB_00555"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5605307.1"
                     /translation="MNTRAFLLAGILMTGSVHAVPPCPGTSWPPPFRFDYDARATRGF
                     LSLSGDSMLTLTRDADGYALVSETTAGVWFSARQSSSGRFAEAGVVPTAYSERNGGRP
                     LMTTRLDWADGRVSFSASNEIVPTQPQMQDRLSLLLQLGWALRAKAATFALPVAGVRG
                     SSIYHFEPRGAETVETPAGRFQTVKLERPLDASDDRLEVWLAPSLCSLPVKVRFTDRK
                     GMVITNELRAANFQ"
     gene            complement(6011..7171)
                     /locus_tag="AC16HB_00560"
     CDS             complement(6011..7171)
                     /locus_tag="AC16HB_00560"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5605308.1"
                     /translation="MSSRAAPPPASIAAGAHGWIGRAALVLALSLIAHAIVLHLGQRA
                     LKVPPLGFGADRLIEVTLHAPPPLPPPVAAEPKPVPTRVAIPRSDPRPAPAPRPAPAP
                     VVEATPSAPMEQTQAEPESESPVAPTQEAPMLPANSERPQADLSIAGDFEASGATLLA
                     ELEHPPLQRAFLPVSARYVYQTSDTRFAAVTGTTTVQWRFADDGTYDAHLLTTVFGLT
                     ALELRSTGRVQHFGLAPERYTERTVGRAEWATNFDWTNRRVTFSSKNVERELREGIQD
                     RLSFQFQLMALGQRLLSRFRPGASIAMTVGSRDDNATYRFVVIGPERLQTGAGEFDTV
                     KLERPKSADGRDSRIEVWLAPESAWLPVKLRFTDRRNEVTENVLAEAVIPAQ"
     gene            7255..>7948
                     /locus_tag="AC16HB_00565"
     CDS             7255..>7948
                     /locus_tag="AC16HB_00565"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5605309.1"
                     /translation="MKLATLRDGSRDGQLVVVSRDLATAHFAAGIAGRLQTALDDWAF
                     HAPQLDELYQSLNEGRTRHAFAFDPAQCMAPLPRAYQWADGSAYVNHVELVRQARGAE
                     MPPSFWTDPLMYQGGSDDLLGACDDIVCASEDFGIDFEAELAVITSDVPMGADSAHCS
                     GQIRLLMLVNDVSLRNLIPGELAKGFGFFQSKPASGFSPVAVTPDELGAAWRDGKVHL
                     PLVVHWNGVKVGE"
BASE COUNT         1322 a         2689 c         2712 g         1225 t
ORIGIN      
        1 ctcggcgtcg ccgtgctgat cgaacgccgc aaggacccaa gcccttggga gccgtggcgc
       61 ttccgcatcg cagacgtgat gatcgatgaa ggacagttcg ggaacgccgc gcgcacactg
      121 cgtgacgacg gccgcatgac gctcacgctg catccgggaa tgtccgtcac acttttccgc
      181 gatgagtccg aggggtacta cctgaatctc acgtccggcg cgcccgtctg gttcgtgatg
      241 tggcggattg acgacggcga cccgtcgcgc gcctggccgg agatcgtgac gctgtcgtac
      301 aacgaagccg ggcgcctgct cgacgcacag gagcgcgttg acaacctgcc gctgcccgcg
      361 gcgatcaagg actggctcgg ggctttcacg gatgaacatt accggccgga accgaagcaa
      421 cgccgccgac cggcgtcgtt catcgcgcca tcgcggcgct gaagcgtcat gacagaccac
      481 gaaaaggacg aaggctttct gtcgcgttgg gcgcgccgca aggccgaggc gcgccgcggc
      541 ggtgtcgcgc cggccgagtc tgcgccggga aatgcggcgc ctgcggagcg gaccgcgcgc
      601 gccgacgagc gccctcccgt cgcgccgacc gaaccggaag ggacgccgcc cgcccaggac
      661 ccaccgacgc tggcggacgt cgaagtgctg accccgcagt ctgatttcac gcgcttcgtg
      721 tcggtcggag ttgaccccga ggtgaagaac gccgcgctga agaagatgtt cgccgaccct
      781 cacttcaacg tgatggacgg gctcgacacc tctatcgacg actactcacg gccagatcct
      841 atcccggccg acatgcttgc caagatgacg cagtcggagc acctcgaact cgcgagccgg
      901 cgtctcgcag ccccaccttc gccggtcgac gaggtcgcac ggcgcactgc gtccgatgtc
      961 gcgccctccg ctgccgacga cgcgcccctt ccggaaccga tcgctgccga tgaaaacgtt
     1021 gatctgcaac tgcaaccgca cgatgccgct ggacgcgccg gcgctgaacc aggcgctgcg
     1081 gaagacatcg ggcgcgagca gcgacgggct cgataagacc cacaccctgt tgtgtcgacg
     1141 cgagttcggg gcgttcctgc aggccgcgga ggaaaccgca gcgaccgggg atgccctggt
     1201 ggtcgcctgc acgcaggaaa gccgcctgtt tctggatctc gatgccgagt cccgcggcgc
     1261 ggcaggccgg agccagccga tcaggttcgt caatatccgc gagacgggtg gatggtcgcg
     1321 cgacgcggcg tcggcgatgc cgaagctggc ggcgctgatc gccgccgcgc aaatgcccct
     1381 gccggctccg accaccacgg tcagctatcg atccggcggc cgttgtctgg tgatcggtac
     1441 agccgacgcc gtcgagcgcg cggccgcgat gctggccgat cggctcgacg tcagcctgct
     1501 cgtcgaggga accggtggcg caatcgagca gcgacacgat cgcgccgttt acggcggccg
     1561 tctggtccgg ctgtcggggt ggctcggcgc cttcgaggcg gaatgggaga gccgtaatcc
     1621 gatcgatctc gaactgtgca cacgctgcaa tgcatgcatc gcggcctgtc ccgagcacgc
     1681 gatcgacttc agctatcaga tcgacctcgc ctcatgccgc agccatcgcg agtgcgtgcg
     1741 cgcgtgcggt gccgccggag cgatcgactt cgaccgcccg tcgcgtgcga cgcaggaacg
     1801 attcgacctg gtcctcgacc tgcgcgcgcg gcccgcgttc gtgctgcatc agccgccgca
     1861 aggctatttc cacgtcgggg ccgatgagcg cgcgctggtg aaggcggtac ttgcattgcg
     1921 cgagctcgcc ggcgagttcg aaaagccgaa gttctattcc taccagcaga acctgtgtgc
     1981 gcacagccgt aacgagaaga ttggctgcac cgcgtgcatc gacgtctgtt cggcgcgcgc
     2041 gatccgcagc gacgccgcgc tgaagggcaa gccacgcacc gccgcgccgc cacgcggacc
     2101 cgatgccgcg cccctcctgc agccaagcgg cggcgggatc gtcgtcgagc cgcacctgtg
     2161 cgtaggctgc ggcgcctgct cgaccgtgtg tcccagcggc gcgatgtcgt tcgcctaccc
     2221 cgcgctcgcc gatcaaggac gtcgcctgcg cacgctgctg gccgcctatg ccggtgccgg
     2281 cgggcgcgac gcggcgctgt tgctccacag cgagggcgcc ggcgcccggc tgatcgacga
     2341 tctcggccga gcggcacgca gcgatcgatc gtttcatggc gtgccggcgc gggtactgcc
     2401 gttgtccgtc tggcatacgg cgagcgtcgg catcgaactg tggctgactg cgatcgcgca
     2461 aggcgcgagc aacgtgtggg tgctggtgac cggggaagag gcgccggagt atcggcaggc
     2521 gctcggcgag cagatggccg tcgcgcaggc gatcctgagc gggctcggct acgaaggcac
     2581 ccacttccgg ctgctcgaag cgcgcgacgc gcgcgacctg ccggcactgg atgccgcgct
     2641 gcgcgaaccg ccggcgcgga cggtggcagt tgccgccgcc tatgcagcgc aggccaacaa
     2701 gcgcacgacg ctcgaactcg ccgtcgacca tttgctggcc caggcgccgg tgcgcgccga
     2761 cgacattgcg ctgcccgccg caggggcgcc gttcggcgcc gtgcaggtca actcggagac
     2821 ctgcacactg tgcctgagct gcgtcggcgc ctgtcccgcg agcgcactgg ccgacaaccc
     2881 cgagcgaccg cagctcggct tcatcgagat gaactgcgtg cagtgcggac tgtgcgcgag
     2941 tacgtgtccg gaacgtgcga tcacgctgca gccgcggctg tggctcgccg acgggggcaa
     3001 ggcgcgccgg gcaactcgca tcctgcacca gatggagccg tatcgctgcg tccgctgcgg
     3061 caagccgttc ggcacggtac gcgcgatcga gaagatgctc gcgacgctgg gagctcattc
     3121 cgccttccag ggcgcggccg tggagcgcct gaagatgtgc aacgattgcc gcgtgatcga
     3181 catgtacacg agcgagcacg aggtccgcat caccgaactt tgagccaacg tcatgcgaac
     3241 ctcgactttg cagttcaacc agcccgacga cgtcgaagag atttctcgcg ccgacctgta
     3301 cggattgctg tcgcgccttt ggctcgcggc gcccgacgag gcgctgctcg ccaggatgcg
     3361 cccggggacg agcggagcgg gcgatgcgga aggtcagctg gccgcgccgt ggcgggcgct
     3421 gatcgcggcg ctgcgcgcga ccgatgcgaa cgccgccagc gtcgaatacg acgcgctgtt
     3481 ccagggcgtc ggcaagccgg aggtcatgct gtacggctcg tactacgtga ccgggtttct
     3541 caacgaaggg ccgctggtgc ttctgcggag cgaattgcgc gatctcggcc tcgcgcgtga
     3601 cgagacacgc gccgagaccg aggatcacat tgccttcctg ttcgaagtga tgcgctacct
     3661 ggtcgcgggc gacgatgccg ccgtgtgcaa tctagaacga cagcgccgct ttttccgcgc
     3721 gcatctgcaa ccgtgggtcg agcggctgtg cgacgcggtc gaggcgcatc cgggcgcgcg
     3781 cttgtggcgc gcgatcgccg gactgacgcg tgcgttcatg caggtcgaag cgcagggatt
     3841 cgatatgctg gaagcatgag catccggcgt gacgacatca cgggcctgat cctcgcgggc
     3901 gggcgcggca cccgcatggg aggcgtggac aagggcctgc agaactaccg cggcatgccg
     3961 ctcgcgctgc acgcgctgct gcgcctcgcg ccgcaggtcg gccaggtgat gatcagcgcc
     4021 aaccgcaatc tggctgcgta tgaatcgatg ggcgtaccgg tctggcccga tccgctgccg
     4081 gactatgccg gtccgctcgc cgggtggctg gcgggcctgg aacgctgcac gacaccgtat
     4141 ctgatcgccg tgccgtgcga cacgcccgcg tttccggccg atctggttga gcggctttcc
     4201 gcgggactcg ccgccgccga cgccgaaatc gcaatggccg ccacgcgcga tgcaggcgca
     4261 gtgcggacgc aacccgtttt cagcctgatg aagtccgacc tgctcgaaag cctgagcgcg
     4321 tttcttcgct cgggccagag caagatcatg cgctggaccg ggcagcgacg ctgcgtcgaa
     4381 gtcgtgttcg acgatgccag cgcgttcttc aacgccaata ccctggacga gctgcagcag
     4441 ttgcaggaag cgcaccctga cagcgctcgg ggaacgtagc ggccgatgaa caagaccatc
     4501 aaacgctatc ggctggacgg tggcccggtc aggctgaagc atttcgatcc cggcgctcgc
     4561 ccatactcca ccggcggctc gcgcgaagaa gacaaggcgc ggaccgccga gctggcgcac
     4621 gagcttgcgt cgctgcagga caatttctac gcgctgcgca aggaacggct gctgatcgtg
     4681 ctgcagggga tggacacgag cggcaaggac ggcacgatcc gctcggtgtt ccaggcaatc
     4741 gacccgctgg gcgtgcgcgc ggtgtcgtac cgggccccga ccgatccgga gaagcagcgc
     4801 gacttcctgt ggcgccacca tcgcgacgtg ccgggcgtgg gcgagatcgt gatcttcaac
     4861 cgctcgcact acgaggcggt gctgatcgaa tacgtgcacg gctggatcga caaggacgaa
     4921 cgcaagcggc gattgaagca catcaatgcg ttcgagcgcc tgctgaccga taccggcacc
     4981 acgatcgtca aatgcttcct gcacatcagc caggacgagc agcgccgccg gctgcaggag
     5041 cgcatcgacg atccggacaa gaactggaag ttcaacctgc aggacctgga ggagcgcaag
     5101 ctctgggacg actaccagcg cgcctacgag gtcgctctcg aggagaccgc cacgacgact
     5161 gcgccatggt tcgtggtgcc gtccgacagc aagacgcacc ggaacctgat ggtgacgacg
     5221 atcctgctcg acacgctgcg gcgcctggcg ccgaagccac cgcccgcggt caagtcgctg
     5281 gcgggtatca aggtgcgcta gccgcgccta ctggaagttc gcggctcgca gttcgttggt
     5341 gatcaccatg cccttgcggt cggtgaagcg caccttcacc ggcaggctgc acagcgacgg
     5401 tgccagccag acctccaggc gatcgtcgct ggcgtcgagc gggcgctcca gcttgaccgt
     5461 ctggaagcga ccggcaggcg tttcgacggt ctccgcgccg cgcggctcga agtgatagat
     5521 cgacgacccg cgcacgccgg ccaccggcag cgcgaatgtc gccgccttcg cgcgcagcgc
     5581 ccagccaagc tgcagcagca gactcagccg atcctgcatc tgcggctgcg tcggcacgat
     5641 ctcgttgctg gccgaaaagc tcacgcgccc gtctgcccag tcgagtcgcg tcgtcatcag
     5701 cggtcggccg ccgttgcgct cggaataggc ggtcggcacc acacccgctt cggcgaacct
     5761 tccactcgat gactggcgcg cgctgaacca cacgccggcg gtcgtttccg acacgagcgc
     5821 atagccgtcg gcgtcgcgcg tcaaggtcag catgctgtca ccgctcaggc tcagaaagcc
     5881 acgcgtggcg cgcgcgtcgt agtcgaaccg gaatggcggc ggccacgacg tgccgggaca
     5941 cggcggcacc gcgtgcaccg aacccgtcat cagaatgccc gccagcagga aagcacgcgt
     6001 gttcacaagg tcattgcgcc gggattacgg cttcggccag cacgttctcc gtcacctcgt
     6061 tgcggcgatc ggtgaaccgc agcttcaccg gcagccatgc cgattccggc gccagccaca
     6121 cctcgatgcg cgagtcgcgg ccgtcggcgc tcttcgggcg ctcgagcttg acggtgtcga
     6181 actcgcccgc accggtctgc agccgctcgg gaccgatcac gacgaagcga taggtcgcgt
     6241 tgtcgtcccg gctgccgacg gtcatcgcga tcgaggcgcc ggggcgaaag cggctcagca
     6301 gccgctgacc cagcgccatc agctggaact ggaacgacag tcggtcctgg atcccctcgc
     6361 gcagttcgcg ctcgacattc ttcgaactga acgtcacgcg ccggttcgtc cagtcgaaat
     6421 tggtcgccca ctcggcgcga ccgacggtgc gctcggtata gcgctcgggc gcgaggccga
     6481 agtgctgcac gcggcccgtc gatctcaatt cgagcgcggt cagtccgaac accgtcgtca
     6541 gcagatgcgc gtcgtacgtg ccgtcgtccg cgaagcgcca ttgcacggtc gtcgtaccgg
     6601 tgacggcggc gaagcgcgtg tcggacgtct gatacacata gcgcgccgac accggcagga
     6661 acgcacgctg cagtggcggg tgctcgagtt cggccagcag cgtcgcgccg cttgcctcga
     6721 aatcgcccgc gatcgacaga tcggcctgcg gacgctccga gttcgccggc agcatcggcg
     6781 cctcttgcgt cggcgcgacg ggggattcgg attccggctc tgcctgtgtc tgttccatcg
     6841 gcgcgctcgg cgtcgcctcg acgaccggtg ccggggcggg acgcggcgcc ggggcaggac
     6901 gcgggtccga ccgaggtatc gcgacgcgcg tcggtaccgg cttcggttcc gcagccacgg
     6961 gcgggggcaa tggcggcggc gcgtgcagcg tcacctcgat cagtcggtcg gcaccgaaac
     7021 cgagcggcgg aaccttcagc gcacgctggc cgagatggag cacgatcgcg tgcgcgatca
     7081 gcgacagcgc gagcaccagc gccgcgcgac cgatccatcc gtgcgcgccg gcggcgatcg
     7141 atgcgggtgg cggagcggcg cgtgaactca tcggctgcaa tctaacgatc gatcgggcgc
     7201 ccgcgccgac aggctctaag atagccgcga cccaagccga cgaagaatag acgaatgaaa
     7261 ctcgccaccc tgcgtgacgg ctcgcgcgac ggacagctcg tcgtcgtctc acgcgatctc
     7321 gccaccgcgc acttcgccgc cggcatcgcg ggccgactgc agaccgcgct ggatgactgg
     7381 gcgttccacg caccgcagct cgacgagctg taccagtcat tgaacgaggg ccgcacgcgc
     7441 catgcattcg cgttcgatcc cgcacagtgc atggcgccgc tgccgcgcgc gtaccaatgg
     7501 gcggatggct cggcgtacgt gaaccacgtc gagttggtgc gccaggcacg cggcgccgaa
     7561 atgccgccgt cgttctggac cgatcctctg atgtaccagg ggggctccga cgacctgctc
     7621 ggcgcgtgcg acgacatcgt ctgcgcgtcg gaagacttcg gcatcgactt cgaggccgaa
     7681 ctggcagtga tcacgagcga cgtgccaatg ggcgccgatt ccgcgcactg cagcgggcag
     7741 atccggctgc tgatgctggt gaacgatgtc agcctgcgca acctgatccc gggcgaactg
     7801 gccaaaggtt tcggtttctt ccagagcaag ccggcgtcgg ggttctcgcc ggtggcggtg
     7861 acaccggacg aactcggtgc agcatggcgc gacggcaagg tccatttgcc tctggtggtg
     7921 cattggaacg gcgtcaaggt cggggagc
//
LOCUS       DBNHBY010000020         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_136344, whole genome shotgun sequence.
ACCESSION   DBNHBY010000020 DBNHBY010000000
VERSION     DBNHBY010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_136344"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2280)
                     /locus_tag="AC16HB_00570"
     CDS             complement(<1..>2280)
                     /locus_tag="AC16HB_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR5605310.1"
                     /translation="AYGAAVIVMAFDEQGQADTLARKCDICARSYRLLVEKVGFPPED
                     IIFDPNVFAIATGIDEHNNYAVDFIEAARWIKQNLPLAKVSGGVSNVSFSFRGNDHVR
                     EAIHTVFLYHAIKAGMTMGIVNAGQLGVYEDIEPELRARVEDVVLNRRPDAGERLVQF
                     AEQIKAGGRQIKEDLAWRAEPVEKRLAHALIHGITTHIVADTEECRAQVAARGGRPIE
                     VIEGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLVPFIEAEKAAAGAAAKP
                     KGKVVIGTVKGDVHDIGKNIVSVVLQCNNFEVVNLGVMVPAEKILQTARDEKADMIGL
                     SGLITPSLEEMQHVAREMQRQGFSLPLLIGGATTSRVHTAVKIAPHYTNGPVVYVSDA
                     SRSVGVCQSLVSDDGAKRFIAEVRAEYDKVRAQHAAKKGPELIALAAARANATRIDWT
                     GYAPPKPKFLGRRAFRNYDLAMIAKYVDWGPFFQTWDLHGPFPAILADEVVGEHARQV
                     FAEGEKMLERIVAERWLTANGAVAFFPANAVGDDIELYTDESRREVLFTWHGLRQQTK
                     KPVIDGVPNPNQCLADFIAPKTTGIADYIGLFAVTAGIGLEKREALFLRKHDDYGAIM
                     LKAIADRLAEAFAELMHERVRRDLWGYAAGEALTNEELVRERYRGIRPAPGYPACPEH
                     AVKAKLFDALACAAIGMGLTENFAMTPPSSVSGFYFSHPQAKYFNVGRIGRDQLEDLA
                     RRKAQTVEELERWLASNLS"
BASE COUNT          342 a          797 c          736 g          405 t
ORIGIN      
        1 acgacaggtt cgacgccagc cagcgctcca gctcctcgac cgtttgcgcc ttgcgccgcg
       61 cgagatcctc gagctggtcg cggccgatgc ggccgacgtt gaagtacttc gcctgcggat
      121 gcgagaagta gaacccgctg accgatgacg gcggcgtcat cgcgaagttt tcggtcagac
      181 ccatgccgat cgcggcacag gcgagcgcgt cgaacagctt cgccttcacc gcgtgctcgg
      241 ggcaggccgg gtagccgggc gcgggacgga tgccgcgata gcgctcgcgg accagttcct
      301 cgttggtcag cgcctcgccg gcggcgtagc cccacaggtc gcgccgcacg cgctcgtgca
      361 tcagttcggc gaacgcctcg gccagccggt cggcgatcgc cttgagcatg atcgcgccgt
      421 agtcgtcgtg tttccgcagg aacagcgctt cgcgcttttc gagcccgatg ccggcggtga
      481 ccgcgaacag gccgatgtag tcggcgatgc cggtggtctt cggcgcgatg aaatccgcca
      541 ggcactggtt cggattcggc acgccgtcga tgaccggctt cttcgtctgc tggcgcaagc
      601 catgccaggt gaacagcacc tcgcgccgcg attcgtctgt gtagagctcg atgtcgtcgc
      661 cgaccgcgtt cgccggaaag aacgcgaccg cgccgttggc ggtcagccac cgttcggcga
      721 cgatgcgctc gagcatcttc tcgccctcgg cgaacacctg gcgcgcatgc tcgccgacca
      781 cttcgtccgc gaggatcgcg gggaacggtc cgtgcagatc ccaggtctgg aagaacggac
      841 cccagtcgac gtacttggcg atcatcgcca ggtcgtagtt gcggaaggcg cgacggccga
      901 ggaacttcgg cttcggcggc gcgtagccgg tccagtcgat ccgggtcgcg ttggcacgcg
      961 ccgcggcgag cgcaatcagc tccgggccct tcttcgcggc gtgctgcgcg cggaccttgt
     1021 cgtattcggc ccgcacctcc gcgatgaacc gcttggcacc gtcatccgac accaggctct
     1081 ggcatacgcc gacggagcgc gacgcgtcgg acacgtacac gaccgggccg ttggtgtaat
     1141 gcggagcgat cttcaccgcg gtatgcacgc ggctcgtcgt cgcgccgccg atcagcagag
     1201 gcaggctgaa gccctggcgc tgcatctcgc gcgcgacgtg ctgcatttcc tccagcgacg
     1261 gcgtgatcag tcccgacagg ccgatcatgt ccgccttctc gtcgcgcgcg gtctgcagga
     1321 tcttctccgc tggcaccatg acgccgaggt tgacgacctc gaagttgttg cactggagca
     1381 cgaccgacac gatgttcttg ccgatgtcgt gcacgtcgcc cttcacggtg ccgatcacga
     1441 ccttgccctt cggcttcgcc gccgcacccg cggcggcctt ctccgcctcg atgaacggca
     1501 ccaggtgcgc gaccgcctgc ttcatcacgc gcgccgactt cacgacctgc ggcaggaaca
     1561 tcttgccggc cccgaacaga tcgccgacga tgttcatgcc gtccatcagc ggcccctcga
     1621 tcacttcgat cggccggccg ccgcgcgccg ccacctgcgc gcggcattcc tcggtgtctg
     1681 cgacgatgtg cgtggtgatg ccatggatca gcgcgtgcgc cagccgcttc tccaccggct
     1741 cggcgcgcca cgccagatcc tccttgatct gccggccacc ggccttgatc tgctcggcga
     1801 actggaccag gcgctcgccc gcgtcagggc ggcgattgag cacgacgtct tcgacgcgcg
     1861 cgcgcagttc gggctcgatg tcctcgtaca cgccgagctg tccggcattg acgatcccca
     1921 tcgtcatgcc cgccttgatc gcgtggtaca ggaacaccgt gtggatcgcc tcgcgcacgt
     1981 gatcgttgcc gcggaagctg aagctgacgt tggagacgcc gccgctcacc ttggccagcg
     2041 gcaggttctg cttgatccag cgcgcggcct cgatgaaatc gaccgcatag ttgttgtgct
     2101 catcgatgcc ggtggcgatc gcgaagacgt tcgggtcgaa gatgatgtcc tcgggcggga
     2161 acccgacctt ctcgaccagc agccggtacg agcgcgcgca gatgtcgcac ttgcgcgcca
     2221 gcgtatcggc ctggccctgc tcgtcgaagg ccatcacgat caccgcggcg ccataggcgc
//
LOCUS       DBNHBY010000021         1643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_204134, whole genome shotgun sequence.
ACCESSION   DBNHBY010000021 DBNHBY010000000
VERSION     DBNHBY010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_204134"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16HB_00575"
     CDS             complement(<1..191)
                     /locus_tag="AC16HB_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5605311.1"
                     /translation="MLIGTAGHIDHGKTSLIRRLTGRNTDRLPEERRRGISIELGYAY
                     VPVGADVLGFVDVPGHEKFV"
     gene            complement(185..1630)
                     /gene="selA"
                     /locus_tag="AC16HB_00580"
     CDS             complement(185..1630)
                     /gene="selA"
                     /locus_tag="AC16HB_00580"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR5605312.1"
                     /translation="MSDLTLPGAGEASGSGPVPATSRAQIPSVDRLLSRKALADLIAI
                     HGRAHVKRAAQAALADVRAALEAGGAAPEAQQLDRDIAARIAQSIASRLRPVFNLTGT
                     VIHTGLGRALLADEAIEAVAAAARGYTALEYDLATGGRGDRDDIVAGLLRELTSAEAA
                     TVVNNCAAAVLLTLKALAAKKEVVISRGELIEIGGAFRMPDIMKAAGCRLVEVGTTNR
                     THARDFEAAITRKTGLVVKAHTSNYAVTGFTAAVEAIDLARIAHERGVPLAIDLGSGA
                     LVDLVRFGLPREPLPQDALRAGADLVMFSGDKLLGGPQAGIIVGRADLIGKIKRDPLK
                     RALRVSKLTLAALEATLRIYASAHRLPERLPTLALLARSRDDIRACCERLRPALAVFA
                     GDGLEVRVADCASQIGSGAQPVERLPSMALVIAPKGKARGRVLEALEARLRGLPTPVI
                     GRISDGALWLDCRCLLPRDEAAFIASLGATC"
BASE COUNT          249 a          586 c          571 g          237 t
ORIGIN      
        1 acgaacttct cgtggccggg cacgtcgacg aagccgagca cgtcggcgcc gaccgggaca
       61 tacgcgtagc cgagttcgat cgagatgccg cgcctccgtt cctccggcag ccggtcggtg
      121 ttgcgccccg tgagccgccg gatcaacgac gtcttgccgt ggtcgatgtg gccggcagtg
      181 ccgatcagca tgtcgcgccc aggcttgcga tgaacgccgc ctcatcgcgc ggcagcaggc
      241 aacggcagtc gagccacagc gcgccgtcgc tgatgcggcc gatcaccggc gtgggcagcc
      301 cgcgcagccg cgcttcgagc gcctcgagca cgcgaccgcg cgccttgccc ttcggcgcga
      361 tcaccagcgc catggacggc agacgctcga ccggttgcgc gccgctgccg atctgactcg
      421 cacaatcggc gacccggact tcgaggccat cgccggcgaa caccgccagg gccggtctca
      481 accgctcgca gcaggcgcgg atgtcgtcgc gcgagcgagc cagcaaggcg agcgtcggca
      541 gccgctcagg caggcgatgc gccgacgcgt agatgcgcag cgtggcctcg agcgcggcca
      601 gcgtgagctt ggagacgcgc agagcgcgct tgagcggatc gcgcttgatc ttcccgatca
      661 ggtcggcacg gccgacgatg attccggcct gcggaccgcc gagcagcttg tcgccggaga
      721 acatcaccag atcggcgccc gcgcgcagcg cgtcctgtgg caatggctcg cgcggcagcc
      781 cgaacctcac gagatcgacc agcgcaccgg agcccagatc gatcgcgagc ggtacgccgc
      841 gctcatgcgc gatccgcgcg agatcgatcg cttcgaccgc ggcggtgaag ccggtcaccg
      901 cgtagttcga cgtgtgcgcc ttgacgacca ggccggtctt gcgggtgatc gccgcctcga
      961 agtcacgcgc gtgcgtacgg ttggtggtgc cgacctcgac cagcctgcag ccggccgcct
     1021 tcatgatgtc gggcatacgg aacgcgccac cgatctcgat cagttctccg cgcgaaatca
     1081 cgacctcctt cttcgccgcc agcgccttga gcgtcagcag caccgcggcc gcgcagttgt
     1141 tgacgacggt ggcggcctcc gcgctggtga gttcgcgcag caggcccgcg acgatgtcgt
     1201 cgcggtcgcc gcgaccgccg gtggcaaggt cgtactcgag cgccgtgtag ccgcgcgcgg
     1261 cggcggcgac cgcttcgatc gcctcatccg caagcagcgc gcgaccgagg ccggtgtgga
     1321 tcaccgtgcc ggtgagattg aacaccgggc gcagccgcga cgcgatcgac tgcgcgatgc
     1381 gcgcggcaat gtcgcggtca agctgctgtg cctcgggcgc ggcgccgccc gcttcgagcg
     1441 cggcacgcac atcggcgagg gcggcctgcg ccgcgcgttt gacatgcgcg cgcccgtgga
     1501 tcgcaatcag atccgccagg gccttgcgac tcagcagtcg atcgaccgag ggaatctgcg
     1561 cgcgcgaggt ggcgggaact ggtccggatc cgcttgcctc gcccgcaccg ggcagagtca
     1621 agtcgctcat cgcttctaac cgt
//
LOCUS       DBNHBY010000022         5902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_68637, whole genome shotgun sequence.
ACCESSION   DBNHBY010000022 DBNHBY010000000
VERSION     DBNHBY010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_68637"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..760
                     /gene="mraZ"
                     /locus_tag="AC16HB_00585"
     CDS             335..760
                     /gene="mraZ"
                     /locus_tag="AC16HB_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR5605313.1"
                     /translation="MFQGRSELTLDGKGRLSIPARHRDALQALCESRLTLTRHPDGCL
                     LVYPRPRWESKREEVARLTYQARALQRLLLGSAVDVELDAAGRLLIPSELRGAAGLER
                     DTVLLGMGEHFELWDAGRHASTEQEQLPQVISAVTGFTF"
     gene            773..1774
                     /gene="rsmH"
                     /locus_tag="AC16HB_00590"
     CDS             773..1774
                     /gene="rsmH"
                     /locus_tag="AC16HB_00590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR5605314.1"
                     /translation="MILPPSFTLQHLTVLVESAVDHLVSTPDGIYVDATFGRGGHSRA
                     LLDRLSPRARVIALDRDPQAATVAGTMRDPRFHFVHTAFSRLRATLGLLGIAQVDGVL
                     LDLGVSSPQIDEAARGFSWRHAGPLDMRMDSTQGQTLAQWLASTTVEELTRIIRDYGE
                     ERFAASIAKAIVACRRDGRRTGRTLATTADLAAVVADAIPAKSRKDAGQHPATRTFQA
                     LRIHLNQELEELALVLPQIVAVLKPRGRLVAISFHSLEDRIVKRFIEAHAHPERQVGA
                     NALRLPLRAAQLPQPTLRPLAKVRPGADEVAANPRARSAVMRVAERTDVAVAAEVAS"
     gene            1774..2073
                     /gene="ftsL"
                     /locus_tag="AC16HB_00595"
     CDS             1774..2073
                     /gene="ftsL"
                     /locus_tag="AC16HB_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR5605315.1"
                     /translation="MRRAASVGGIAVLVGGLVASSLSLITSQHRARGLFVELERAQQF
                     AQQLEADGNRLRVELGRVSQPANVEAAARQLGLRTVDSARTAFLPSPDGFKGAAR"
     gene            2070..3872
                     /locus_tag="AC16HB_00600"
     CDS             2070..3872
                     /locus_tag="AC16HB_00600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR5605316.1"
                     /translation="MKQPAAQRFGAVSGRRSSPAARGGERVAFNANPLLVVKLPAWRS
                     KLMLFLLFCGFVALAGRAVFLQGGVSTGYFQRQGEVRYARTLDVPATRGKVTDRNGVV
                     LAASVPAKAIWAIPDDIDATQRELAQLAQLLQMPVAELRRKLANEDRKFVYLRRQVDV
                     EVAKKIEALKLSGIHQSREFKRYYPESTTVAHLIGFTNVEDRGQEGIELALESTLAGR
                     TGHRRVIKDRMGRIVEEDWLLDPIDGRDVTLSIDHRIQYLAYSALRNAIDANRARAGS
                     VLVLDVKSGEVLALASWPSFDPNARGSWNIEALRNRVVTDTFEPGSTLKPFSIAAALD
                     AGRITPQMRVNTAPGRMTIGGRTIGDAHPHGVLTVEEIVEKSSNVGTAKIALELPAQL
                     LWDTYTNVGFGQAPRLGLQGAVPGRLRPYKAWRPIEQATIAYGYGVSVSLLQLARAYT
                     VFARDGDVVPLSLLKTTGPVAGVQVIKPETARAMRHMLELAVSDQGTAPAARIAGYRT
                     AGKTGTARKLRNGRYTNAYIASFSGFAPVSNPRIVVAVMIDEPGGTAYYGGQVAAPVF
                     AQIAAGTLRTLQVPPDGPITLPALPAQPLPEEAL"
     gene            3893..5476
                     /locus_tag="AC16HB_00605"
     CDS             3893..5476
                     /locus_tag="AC16HB_00605"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR5605317.1"
                     /translation="MEILAWLRAQAPAAFAHDLQLDSRRVSTGDVFVAAPGAIPGKSA
                     DGRSYIDAAVARGAIAVIVDADDWQPRDVGVPALPVANLRRVLGQLAARFYALPSEGL
                     LGIGVTGTNGKTSCSQWIAQLLTLAGTRCAVIGTVGSGFPGALRADATLTTPDAVTLQ
                     RAVKTFAHDGARALAMEVSSIGLDQGRVNGMKFDVALFTNLTRDHLDYHGTMERYEAA
                     KTVLFDWPTLTDAVINLDDPAGQRLVQRLASRARAGGVRVTGYTAHGADVPPAVARTL
                     SAGDLRVTPEGLAFAIRVNDAAHAVSVPLVGEFNVSNLLGVVGTVLACGVDLEQACAL
                     LPRLLPPPGRMQRFGGHGAPLVVVDYAHTSDAIEKALGALRPMAQARRGRLWIVFGAG
                     GDRDPGKRAPMGSAAARGADRVVITSDNPRTEDPLRIIRDIAAGVPDGTTTDEIVDRG
                     PAIAHAVQRAAVEDVVLIAGKGHEDYQDVGGVRRPFSDIDEARTALAARGGGNGSGAR
                     RSAGTGPSSSTFFVLRAAC"
     gene            5470..>5902
                     /locus_tag="AC16HB_00610"
     CDS             5470..>5902
                     /locus_tag="AC16HB_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR5605318.1"
                     /translation="MLTVGELAQSLRGATVQGDARTRFKSVATDSRTCATGALFVALR
                     GERFDGHDYVAQAVARGAVAALVEQPVEVAVPQVVVDDSKRALGLAAAAWRARFNLPL
                     IAVTGSNGKTTVTQMIAAILARAVGERKRLATRGNLNNEIGL"
BASE COUNT          927 a         1953 c         2036 g          986 t
ORIGIN      
        1 actgcacagg atccggcgta caggcgggct ttgcgttttt cactggatca acagcagcag
       61 actgtttcgg ctgttgagat ttcactttca cgggttgtca tatccccttc atttgttgat
      121 ctttttcatc atccgttgat tgaaaactcc gcgctaagtc cttgtcacga ttgagaaatc
      181 caactgattc cgcttgaccc ccccgcgtcg cttcgctaca gtttcgtcgt gggaaaaagt
      241 gggaaatggt ggattagtta gtgaatactt cactggaata gtttgccagt gaaagatcta
      301 gctgattcac ccgggggacg gagacggggg gatcgtgttt cagggccggt cggaactgac
      361 gctcgatggc aagggtcgcc tttcgatacc cgcgcgccat cgagatgcgc tgcaagcgct
      421 atgcgagtcg cgcctgacac tgacgcgcca tccggacggc tgtctccttg tctatccccg
      481 cccacgttgg gaaagcaagc gcgaggaagt cgcgcgtctt acttaccagg cgcgagcgtt
      541 gcagcgcctg cttctcggca gcgcggttga tgtcgaactc gatgccgccg ggcgcttgct
      601 gattccttcc gaactgcgcg gcgccgcggg gctcgaacgc gacaccgtgc tgctcggcat
      661 gggcgagcac ttcgagctct gggatgccgg gcggcacgcg tcgaccgaac aggagcaact
      721 cccccaggtg atcagtgcgg tgaccgggtt cacgttctga tggtgagtct ggctgattct
      781 tccgccgtcg ttcacgttgc agcacctgac cgtccttgtc gagagcgccg tcgaccatct
      841 ggtgtcgacg cccgacggta tctatgtcga cgctacgttc gggcgcggcg ggcactcgcg
      901 cgcacttctc gatcggcttt cgccgcgggc gcgtgtcatc gcgctggatc gcgacccgca
      961 ggcagcgact gtcgcaggga cgatgcgcga tccgcgattt cacttcgtgc acacggcgtt
     1021 ttcacgcctg cgcgccacgc tgggcttgct tggaattgct caggtcgacg gcgtgctgct
     1081 ggatctcggc gtgtcgtcgc cacagatcga cgaggccgcg cgcggttttt cgtggcgcca
     1141 tgccggtccg ctcgacatgc gcatggacag cacacagggt cagacgctcg cgcagtggtt
     1201 ggcctcgaca actgttgaag aactgacgcg aatcatccgg gactatgggg aagaacggtt
     1261 tgctgcatcg attgcaaagg cgattgtggc ttgccgccgg gatggacggc gcacgggtcg
     1321 gaccctcgca acgaccgccg accttgccgc tgtcgtggca gacgcgatcc cggccaagag
     1381 ccgcaaggac gccggtcagc atccggccac gcggaccttt caggctctac ggattcacct
     1441 caatcaagag cttgaggaac tggcgctagt gttgccgcag attgtcgccg tcctgaagcc
     1501 gcgcggtcgc ctcgtggcga tcagcttcca ttcgcttgag gatcggatag tcaagcgctt
     1561 catcgaggcg cacgcgcatc cggaacgcca ggtcggtgca aacgcgctgc ggctaccgct
     1621 gcgcgccgcg cagttgcctc agccgacgct gcgcccgctg gccaaagtcc ggcccggcgc
     1681 cgacgaggtt gccgcgaatc cgcgcgcacg cagcgccgtg atgcgcgtgg ccgaacgcac
     1741 cgacgtcgcg gtcgccgcgg aggttgcctc gtgatgcgcc gcgctgcgag cgtcggcggc
     1801 atcgccgtgc tggtcggcgg acttgtcgcg tccagcctgt cgctgatcac gtcccagcat
     1861 cgggcacgcg gcctgttcgt cgaactcgag cgcgcgcagc aattcgcgca gcagctcgag
     1921 gccgacggca atcggctgcg tgtcgaacta ggccgagtgt cgcagccggc gaacgtcgag
     1981 gcggctgcgc gccagcttgg gctgcgcacg gtcgacagcg cgcgcaccgc attcctgccg
     2041 tctcccgacg gtttcaaagg ggccgcgcga tgaagcaacc ggccgcccag cgattcggcg
     2101 cggtcagtgg tcgccgttcg tcgccggccg ctcgcggcgg cgagcgggtg gcattcaacg
     2161 cgaacccgct gctcgtggtg aagctgccgg cgtggcgttc gaagctgatg ctgttcctgc
     2221 tgttctgtgg cttcgtcgcg ctcgctggac gtgcggtgtt cctgcagggg ggcgtgtcga
     2281 ccgggtattt ccagcgtcag ggcgaagtgc gctacgcgcg caccctcgac gtgccggcca
     2341 cgcgaggcaa ggtgaccgat cgcaatggcg tcgtgctcgc ggcgtcggtg ccggccaagg
     2401 cgatctgggc gatccccgat gacatcgatg ctacgcagcg ggaactggcg caactggcgc
     2461 agctgctgca aatgccggtc gcggagctgc gccgcaagct cgccaacgag gatcgcaaat
     2521 tcgtctacct gcgccgacag gtcgacgtcg aagtcgcgaa gaagatcgag gcgctcaagc
     2581 tctccggcat ccaccagtcg cgcgagttca agcggtacta cccggagagc accacggttg
     2641 cgcatctgat cgggttcacg aacgtcgagg atcgcgggca ggaaggcatc gaactcgcac
     2701 tcgaatccac actcgccggc cgcaccggcc accgccgcgt catcaaggac cgaatggggc
     2761 gcatcgtgga ggaggactgg ctgctcgatc cgatcgacgg acgcgacgtc acgctttcga
     2821 tcgatcaccg catccagtat ctcgcgtact cggcgctgcg caatgccatc gatgccaatc
     2881 gcgcgcgtgc cggctcggtg ctggtgctcg acgtgaagag cggcgaagtg ctggcactgg
     2941 cgagctggcc cagcttcgac ccgaacgcgc gcggctcgtg gaacatcgaa gcgctgcgca
     3001 accgggtcgt gacggacacc ttcgagcccg gctccacgct gaagccgttc tcgatcgcgg
     3061 ccgccctcga tgcgggtcga atcacgccgc agatgcgcgt taacacggcg cccggccgca
     3121 tgacaatcgg cggccggacg atcggtgacg cccatccgca cggcgtgctg acggtcgagg
     3181 agatcgtcga gaagtcgagc aacgtcggca ccgccaagat cgcgctcgag ctgccggccc
     3241 agttgctgtg ggatacctac accaatgtgg gattcggaca ggcgccgcgg ctcggcttgc
     3301 agggtgcagt gcccggccgg cttcggccct acaaggcgtg gcgtccgatc gagcaggcaa
     3361 cgatcgcgta tggctacggc gtgtcggtgt cgcttctgca actggcgcgc gcgtacacgg
     3421 tgttcgcacg ggatggcgac gtggtgccgc tgtcgctgct gaagacgacc ggcccggtgg
     3481 ctggagtgca ggtgatcaag cccgagaccg cgcgcgcgat gcgccacatg ctcgaactcg
     3541 cggtgagcga ccaaggcacc gcacccgcgg cgcgcatcgc cgggtatcgg accgccggca
     3601 agaccggcac cgcgcgcaag ctgcgcaacg gccgctacac caacgcgtac atcgcttcgt
     3661 tctccggctt tgcgcccgtg tcgaatccac gcatcgtggt agcggtgatg attgacgagc
     3721 caggcggcac cgcgtactac ggcggccagg tcgcggcgcc ggtgtttgcg caaatcgctg
     3781 ccggaacgct gcgcacgctg caggtgccgc ccgacgggcc gatcacgcta cccgcgctgc
     3841 cggcgcagcc gttgccagag gaggcgctgt gaccggcccg catgccgccg cgatggaaat
     3901 cctggcgtgg ctgcgcgcac aggctccggc cgcgttcgcg cacgaccttc agctcgacag
     3961 ccgccgcgtg agcaccggcg acgtgttcgt cgcggctccg ggtgcgattc cgggcaagag
     4021 tgccgacggt cgctcgtaca tcgacgctgc ggtcgcgcgc ggcgcaatcg cggtgatcgt
     4081 cgatgcggac gactggcagc cgcgcgacgt cggcgtgccc gcactcccgg tcgccaacct
     4141 gcggcgcgtg ctcggccagc tcgccgcgcg cttctatgcg ttgcccagcg aagggctgct
     4201 cggcatcggc gtgaccggca ccaacggcaa gacctcgtgc agccagtgga tcgcacagtt
     4261 gctgacactg gcgggaacgc gctgtgccgt gatcggcacc gtcggcagcg gctttcccgg
     4321 tgcattgcga gcggatgcga cgctgaccac gccggacgcg gtcacgctgc aacgtgcggt
     4381 caagaccttc gcgcatgacg gcgcgcgcgc gctggcgatg gaagtgtcgt cgatagggct
     4441 cgaccaggga cgcgtgaacg gcatgaagtt cgatgtggcg ctcttcacga acctgacgcg
     4501 cgaccatctc gactaccacg gcacgatgga gcgttacgag gcggccaaga cggtcctgtt
     4561 tgactggccg acgctcaccg atgcggtgat caacctcgac gacccggcgg gccagcgact
     4621 ggtgcagcgg ttggcgagcc gtgcgcgcgc cggcggcgtg cgcgtcaccg gttacaccgc
     4681 gcacggcgcg gacgtgccgc cggcggtcgc gcgcacgctg tcggctggcg atctgcgcgt
     4741 cacgccggag ggcctcgcgt tcgcaatccg ggtcaacgac gccgcgcacg cggtatccgt
     4801 gccgctggtc ggagaattca acgtctcgaa cttgctcggc gtggtcggaa ccgtgctcgc
     4861 gtgcggcgtc gatctggagc aggcctgtgc actgctgccg cggctattgc ctccgcccgg
     4921 tcgcatgcag cgcttcggcg gccacggcgc gccgctggtg gtcgtcgact acgcgcacac
     4981 gtccgacgcg atcgaaaagg cgctcggcgc cctgcggccg atggcgcagg cgcggcgcgg
     5041 ccggctgtgg atcgtgttcg gcgccggcgg cgaccgcgat cccggcaagc gcgcgccgat
     5101 gggatccgcc gccgcgcgtg gcgccgatcg cgtcgtgatc accagcgaca acccgcgtac
     5161 cgaggacccg ttgcgcatca tccgcgacat tgcggccgga gttccggatg gcacgacgac
     5221 cgacgagatc gtcgatcgcg gcccggccat cgcgcatgcg gtccagcgcg cggcggtcga
     5281 ggacgtcgtg ctgatcgccg gaaaggggca cgaggactac caggacgtcg gcggcgtcag
     5341 gcgaccgttt tcggacatcg acgaggcgcg taccgcgctc gccgcgcgcg gcggtggcaa
     5401 cggctccggc gctcgccgct cggccggcac ggggccgagc agctcgacat tcttcgtgct
     5461 gagggcggca tgctgacagt tggcgaactc gcccagtcgc tgcgcggtgc gaccgttcaa
     5521 ggcgacgcgc gcacgcgctt caagtcggtc gcgacggact cgcgcacctg cgcgaccggc
     5581 gcgctgttcg tcgcgctgcg cggcgaacgc ttcgatggtc acgattacgt cgcgcaggcc
     5641 gtggcccgcg gcgcagtcgc ggcgctggtc gagcagccgg tcgaggtcgc ggtcccgcag
     5701 gtcgtcgtcg acgacagcaa acgcgcgctc ggactggctg ctgcggcgtg gcgcgctcgc
     5761 ttcaacctgc cgctgatcgc agtgaccggc agcaacggca agaccacggt cacgcagatg
     5821 atcgccgcca tcctggcgcg cgcagtcggt gaaaggaagc ggctggcgac gcgcgggaac
     5881 ctgaacaacg agatcggcct gc
//
LOCUS       DBNHBY010000023         4981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1151417, whole genome shotgun sequence.
ACCESSION   DBNHBY010000023 DBNHBY010000000
VERSION     DBNHBY010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1151417"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16HB_00615"
     CDS             complement(<1..391)
                     /locus_tag="AC16HB_00615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5605319.1"
                     /translation="MTNDGEQAMSINDGSSVQGHRGSLRGIRIRAVRPDDSERIVKAF
                     RQLDPASIYRRFFAPKKALSSEELRRLTQSDGVRNVVLVATVASSNQEIIVGLGDYAR
                     NGTSAEIAFAVEEDYWARGIASELLRQL"
     gene            complement(407..643)
                     /locus_tag="AC16HB_00620"
     CDS             complement(407..643)
                     /locus_tag="AC16HB_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605320.1"
                     /translation="MNKHPQLPKGHTSILSPQFKYVPAVSTDLAATFARIRGERMQPQ
                     PAPDDATVWHGRADVAPGLGIEVLEASWITRWRS"
     gene            complement(640..1911)
                     /locus_tag="AC16HB_00625"
     CDS             complement(640..1911)
                     /locus_tag="AC16HB_00625"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR5605321.1"
                     /translation="MSAARPTPIAAAVAARNGRATPDASSEPILVLNCGSSSIKFALF
                     DADALPLTRRPLWAGKVDGIGTASPCLVEADAAPEPLALNGEQPYHDALQAVRACVQR
                     RAGGRLPAVVAHRVVHGGSKYTQPVAIDRAVLAELKGFIPLAPLHQPYALQAIEVLLA
                     ELPEVSQVACFDTAFHRTLPQVEQMLPLPHALWERGIRRYGFHGLSYEYMAIALAERH
                     GDAARGRTIVAHLGSGASLCAMHRLQSVATTMGFSALDGLMMGTRCGSIDPGVLLHLI
                     ETEGLGVEQLTHLLYHESGLLGVSGSSSSPQPLLAAEATSPRARAALELYVRRIVREI
                     GALTAVLGGLDMLAFTAGIGENSAVLRERICGALDWLGVSIDEAANRANASTISGRLG
                     RVRVAVEPTNEEWIAASHARRVIRGVAMEAA"
     gene            complement(1908..3401)
                     /locus_tag="AC16HB_00630"
     CDS             complement(1908..3401)
                     /locus_tag="AC16HB_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIR5605322.1"
                     /translation="MSTFATDDLEVLRNRTFDEIAVGDRASIERTLTAADIQLFAAMS
                     GDTNPQHIDAEFAASTRFHGVIAHGMWGAALISAVLGTRLPGPGTVYAGQSLQFHAPV
                     RVGDTLTTTVTVTARDAADHRVTLDCRCVNQDGKTVISGEALVLAPTERIERPRGTLP
                     QVRIALAQGDDLVRILQYVRPLGRIKVAVVHPCDAVSLSAALDARAAGVIEPVLIGPR
                     ARIEAVSQKSDLKIDGIAIDDVPHSHAAAARAVELAVKGEVEALMKGSLHTDELMAAV
                     VASPLRTKRRMSHCFLMQTPAYPRPFIITDAAVNIAPTLQDKADIVCNAVELAQAFGL
                     AQPRVAILAAVETVNPNMPATLDAAALCKMADRGQIPGAIVDGPLAFDNAVSAAAART
                     KGIVSEVAGAADILVVPDLESGNMLAKQLMYLGGAASAGIVVGARVPIVLTSRADPRE
                     ARIASCAVALVAARHGAHVAANVGQQIPIGRAAGVATASQTTLERQP"
     gene            complement(3453..4241)
                     /gene="fabI"
                     /locus_tag="AC16HB_00635"
     CDS             complement(3453..4241)
                     /gene="fabI"
                     /locus_tag="AC16HB_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5605323.1"
                     /translation="MHQVDVRSQSLLGGRKGLVVGIANEHSIAYGCARMFRECGAELG
                     VTWIDERTRGYVEPLARELGATIKMQMDVEQPGAMEAVFDAIRERWGRLDFVLHSIAF
                     APPSDLRGRVADTSRAGFTRAMDISCHSFMRVAQLAEPLMDKGGTLLTMTYLGADEVV
                     PRYGVMGPIKAALEASVRYLATELGPRGIRVNAVSPGPIRTRAASGIREFDELLAEAR
                     RRAPLAREVEIDDVGALCAFLVSDAAQAITGDVHYVDGGFHILA"
BASE COUNT          791 a         1682 c         1701 g          807 t
ORIGIN      
        1 tcagttgccg cagcagttca cttgcgattc cgcgggccca gtaatcctct tccaccgcga
       61 acgcaatctc tgcgctcgtt ccattccgcg cgtagtcacc cagaccaacg atgatttcct
      121 gattgctgct tgcgaccgtg gcaactagca caacgttgcg tacgccgtcg ctctgggtga
      181 gccgccgcag ttcctcgctg ctcagcgcct tcttgggagc gaagaaccgc cggtagattg
      241 atgcagggtc cagttgccga aatgctttga cgatgcgttc gctgtcgtcc gggcggacgg
      301 cacgaatgcg tatgcccctc aaactcccac ggtggccctg cacgctcgag ccgtcattga
      361 tactcatcgc ttgctcgccg tcgttcgtca tctgcgctcc ggctcgctag ctccgccatc
      421 tcgtaatcca ggatgcctcc aggacttcga tgccgagccc cggtgcaacg tcagcacggc
      481 cgtgccaaac cgtcgcatcg tcgggcgctg gctgcggctg catgcgttcc ccgcggatcc
      541 gcgcgaaggt ggcggccaga tccgtggaca ccgccggcac gtacttgaac tgcggcgaaa
      601 gaatcgacgt gtggcccttc ggaagctgcg ggtgcttgtt catgccgcct ccattgcgac
      661 gccgcggatc acgcgccgcg catgggaggc ggcgatccac tcttcattcg tcggctccac
      721 cgcaacccgt acgcggccga gccggcccga gatcgtggat gcattggcgc ggttcgcagc
      781 ctcgtcgatt gacaccccga gccagtcgag tgccccgcag atccgctccc gcaacacggc
      841 gctgttctcg ccgatcccgg ccgtgaaggc cagcatgtcg agtccgccca gcacggcagt
      901 caaagcgccg atctcgcgca cgatgcggcg cacgtaaagc tcgagagcag cgcgcgcgcg
      961 gggactggtt gcttccgccg cgagcagcgg ctgcgggctc gacgacgatc cagacacgcc
     1021 gagcagcccc gactcgtggt agagcagatg cgtgagctgc tccacgccaa gcccttcggt
     1081 ctcgatcaga tgcagcagca cgccgggatc gattgagccg cagcgagtgc ccatcatcag
     1141 accgtcgagc gcggagaacc ccatcgtggt cgccacgctt tgcagccggt gcattgcgca
     1201 caggctggcg ccgctgccga gatgggcgac gatcgtgcgg ccgcgcgcgg cgtcgccatg
     1261 ccgttccgca agtgcgatgg ccatgtactc gtaggacaga ccatgaaacc cgtagcgccg
     1321 gatgccgcgc tcccacagcg catggggcaa cggcagcatc tgctccacct gcggcagcgt
     1381 gcggtggaat gcggtatcga agcaggcgac ttgcgacacc tcgggcaatt cggccagcag
     1441 aacctcgatc gcctgcagcg cgtagggctg gtgcagcggt gccagcggga tgaagccttt
     1501 gagttcggcc agcactgcgc ggtcgatggc gaccggctgg gtgtacttgc tgccgccatg
     1561 cacgacccgg tgcgccacca cggcgggcag ccgcccgccg gcgcgccgtt gcacgcacgc
     1621 gcgcacggcc tgcaaagcgt cgtggtacgg ctgctcgcca ttcagggcga gcggttccgg
     1681 tgcagcgtcg gcctcgacca ggcacgggct ggcggtgccg atgccgtcga ccttgccggc
     1741 ccacaggggt ctgcgcgtca acggcagcgc atcggcgtcg aacaaggcga acttgatgct
     1801 ggaggagccg cagttcagaa ccaggatcgg ttcgcttgat gcatccggcg tcgcacggcc
     1861 gttgcgtgcg gcaacggcgg ctgcgatcgg ggtcggccgg gcggcgctca tggctgacgc
     1921 tccagcgttg tctgcgaggc ggtagcaacg cccgccgccc ggccaatagg aatctgctgg
     1981 cccacattgg cagcgacgtg ggcgccgtgc cgcgcggcca ccagcgccac cgcgcacgag
     2041 gcgatgcgcg cttcgcgcgg atcggcgcgg ctggtgagca cgatcggaac gcgcgcaccg
     2101 acgacgatgc cggcgctcgc cgcgccgccg aggtacatca actgcttggc gagcatgttg
     2161 ccgctctcca gatcgggaac gaccaggatg tccgcggcgc cggcgacctc cgagacgatc
     2221 cccttcgtgc gcgcggcggc agcggagacg gcgttgtcga atgcaagggg cccatcgacg
     2281 atggcgccgg ggatctggcc gcggtccgcc atcttgcaca gcgccgccgc atcgagcgtc
     2341 gctggcatat tgggattgac cgtctcgacg gcagcaagga tggcgacgcg cggctgcgca
     2401 aggccgaatg cctgcgccag ttccaccgcg ttgcagacga tatcggcctt gtcctgcagc
     2461 gtcggcgcaa tgttgaccgc cgcgtcggtg atgatgaacg gccgcggata cgccggcgtc
     2521 tgcatcagga agcaatggct catgcggcgt ttcgtacgaa gcgggctggc gacgacggcg
     2581 gccatcagct cgtccgtgtg caggctgccc ttcatcaacg cttcgacctc gcccttgacg
     2641 gcgagctcga cggcacgtgc ggccgcggca tggctgtgcg gcacgtcatc gatcgcgatg
     2701 ccgtcgatct tcagatccga cttctgcgaa acggcttcga tacgcgcacg cgggccgatc
     2761 agcaccggct cgatcactcc ggcggcccgc gcgtcgagag cggcggagag gctgacggcg
     2821 tcgcacgggt gaacgaccgc caccttgatg cgcccaaggg gccgtacgta ctgcagtatc
     2881 cgcacgaggt catcaccctg cgcgagtgca atgcgcacct gcggcagcgt gccgcgcggg
     2941 cgctcgatgc gctcggtcgg cgcaagcacc agcgcctcgc ccgagatgac ggtcttgccg
     3001 tcctggttga cgcagcggca atccagcgtc acgcggtgat ccgcggcgtc gcgcgcggtc
     3061 accgtgacgg tggtcgtcag ggtgtcgccg acgcggaccg gggcatggaa ctgcagcgac
     3121 tggccggcgt agacggtgcc gggcccaggg agccgcgtgc ccagcaccgc ggagatcagc
     3181 gccgcgcccc acatgccgtg ggcgatcacg ccgtgaaacc gcgtcgaagc cgcgaactcg
     3241 gcgtcgatgt gctgcgggtt ggtgtcgccg gacatcgcgg cgaagagctg gatgtccgcg
     3301 gcggtcagcg tgcgctcgat gctggcgcgg tcgccgacgg caatctcgtc gaaagtccgg
     3361 ttgcggagga cctcgaggtc gtccgttgcg aatgtgctca tctcgtttgt gccttgtggt
     3421 cgaatgcttc gggagtgacg gcgccggccg cttcaggcga ggatgtggaa gccgccgtcc
     3481 acgtagtgga cgtcgccggt gatggcctgc gcggcgtcgc tcaccaggaa cgcgcacagc
     3541 gcgccgacgt cgtcgatctc gacttcgcgc gccagcggcg cgcggcggcg cgcttcggcg
     3601 aggagttcgt cgaattcgcg gatcccggag gccgcgcggg tgcggatcgg gccgggcgaa
     3661 acggcattga cgcggatgcc gcgcgggcca agttcggtcg ccaggtagcg caccgaggcc
     3721 tcgagggcgg ccttgatcgg acccatgacg ccatagcgcg gcaccacctc gtcggcgccg
     3781 aggtaggtca tggtcagcag cgtgccgcct ttgtccatca gcggctccgc gagctgcgcc
     3841 acgcgcatga acgaatggca cgagatgtcc atggcgcgcg taaagcctgc gcgcgaggtg
     3901 tcggcgacgc ggccccgcag gtcgctcggg ggtgcgaagg cgatcgagtg cagcacgaag
     3961 tccagccgcc cccagcgctc gcggatcgcg tcgaacaccg cttccatggc gccgggttgc
     4021 tcgacgtcca tctgcatctt gatcgtcgcc cccagttcgc gcgccagcgg ctcgacatag
     4081 ccgcgggtcc tctcgtcgat ccaggtcacg ccgagctcgg cgccgcattc gcggaacatg
     4141 cgcgcgcagc cgtaggcgat gctgtgctcg ttggcaatgc cgaccacgag gcccttgcgg
     4201 ccgccgagca gcgattgcga tctcacgtcg acttggtgca tctacttctc ccgtacgtag
     4261 cggcctggcg catcccggag cgggtgctcg cgaggccggc ccatgcgcgg cggcttgacg
     4321 gacctgccgg agtgcgcgcg cagccactca tgcaaggcgg gccaccatga gcccaggttg
     4381 tcgggtgcat cggccagcca gcggtcgggg tcgagacggg tagagccgct attggaggat
     4441 tggaaaggtc catcgtttat cgcccgcctg gtccagcgat tgctctttgc agggcgcacc
     4501 gcgttgccgg aatgtcatgc cgcacacgtt aggcggcgcg accgaggaag tggtgagcaa
     4561 accgtcgacg tttggtcgcg atttgcatcg acttgtccac ttgatccacg cgacgaaggg
     4621 caagggaacg cgaggcaaag cgaagcgcgt tgcgcgatcc ccgggcgacc ggcagcgcac
     4681 aaccccgacg cgatcgcgac gtttcgctta cgacttcagt gcgcgcacgc cctagcatgc
     4741 attgcgtggc agacggcgat tgcacgccgc gcatgactgc ccggccactg aagaagaccc
     4801 agcgcgtgca tcgatgaatg gtgcacgccg aaaccgagcc cgcaacatgg accgaccccc
     4861 gatcgaccgg tacggaccca gtccggccga gacccgcgct ccctgggcct tggatctcag
     4921 gctcgcgccc ggctcgttct atcgcacgtt gatcatcgtt ctggcggtct ggatcctgca
     4981 c
//
LOCUS       DBNHBY010000024         1774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_542283, whole genome shotgun sequence.
ACCESSION   DBNHBY010000024 DBNHBY010000000
VERSION     DBNHBY010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_542283"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC16HB_00640"
     CDS             <1..732
                     /locus_tag="AC16HB_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5605324.1"
                     /translation="LSLGLLGSGGSILTVPVLHYLLDQPEQAAIGGSLLVVGVIAASA
                     AVPYARIGQVAWRNVVGFGLPGMVGALLGATLARWVPGVAQLLLFAFVMFVAAWKMLA
                     GRALRAPDEQHATRSGAVMAGGFGVGALSGVVGVGGGFLIVPALVLLAGVPMTQAVGT
                     SLVVISLNAFSGFARYQGVLAAKGIELDYATLLAIAAVGVVGSLAGHRLGRHLPQAAL
                     RRIFGAALLALATGITLDVVRRLAA"
     gene            complement(763..1512)
                     /locus_tag="AC16HB_00645"
     CDS             complement(763..1512)
                     /locus_tag="AC16HB_00645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5605325.1"
                     /translation="MIEIIGSYVSPFVRKALVALELKGVGYRIDPITPYIGDDRFSQL
                     SPLRRVPVLLDDGFVLNDSSVILQYLDERYPERHPLLPAAVADRAQARWLEEYADTRV
                     ADIIVWKIFDKAVLQPGIWGQPRDLQGIAQAMQEDLPPVLDYLESKVPEDGFLFGVPG
                     LGSADISLAAPFRNLGFARQRVDAERWPRMAAYVDRVLAQPAFVNLQAYEERQVRTPL
                     AGQRAALAAMGAPLTSQSYAGAEPRRGLPRG"
     gene            complement(1526..1771)
                     /gene="ubiK"
                     /locus_tag="AC16HB_00650"
                     /gene_synonym="yqiC"
     CDS             complement(1526..1771)
                     /gene="ubiK"
                     /locus_tag="AC16HB_00650"
                     /gene_synonym="yqiC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HIR5605326.1"
                     /translation="MIDDLARRLAGSVPENLVALRRDLEQNFKGVLQSQLARLDLVTR
                     EEFDVQSAVLRRTREKLTALEQRLAELEGKAPGTAGG"
BASE COUNT          238 a          617 c          626 g          293 t
ORIGIN      
        1 ctcagcctcg gcctgctggg atcgggtggc tcgatcctca cggttccggt cctgcattac
       61 ctcctcgacc agcccgagca ggccgccatc ggcggctcgc tgctggtggt cggcgtgatc
      121 gccgcgagcg cggccgttcc ctacgcgcgc atcggccagg tcgcgtggcg caacgtcgtc
      181 ggtttcgggc tgcccggcat ggttggcgct ctgctcggcg cgacgctcgc ccggtgggtg
      241 cccggcgtcg cgcaactgct gctgttcgct ttcgtcatgt tcgtcgccgc ctggaagatg
      301 ctcgccggcc gggcactgcg cgcgccggac gagcagcacg cgacgaggag cggcgccgtg
      361 atggctggcg gcttcggcgt cggcgccctg agcggcgtcg tcggcgtcgg tggcggcttc
      421 ctgatcgtgc cggctctggt gctcctggct ggcgtaccga tgacgcaggc ggtcggcacg
      481 agccttgtgg tcatctccct gaatgctttc tcgggattcg ccaggtacca gggcgtgctg
      541 gcagccaagg gcatcgagct ggattacgcg acgctgctcg cgatcgcggc ggtcggcgtt
      601 gtcggcagcc tggccgggca tcgactcggt cgtcacctgc cacaggctgc gttgcgccgg
      661 atctttggcg ccgcgttgct ggcgctcgcc accggcatca cgctggacgt ggtgcgacga
      721 ctcgctgcgt gaacgatccg ccccgcctga gcggggcggc gatcagccgc gcggcaggcc
      781 gcgccgcggc tccgcgccgg cgtaagactg gctcgtcagc ggcgctccca tcgcggcgag
      841 cgcggcacgc tgtcccgcga gcggcgtgcg cacctgccgc tcctcatagg cctgcaggtt
      901 cacgaacgcc ggctgggcga gcactcggtc gacgtaggcc gccatccgcg gccagcgttc
      961 cgcgtcgacg cgctgccgcg cgaagcccag attgcggaag ggcgccgcca gcgagatatc
     1021 ggcactgccg agacccggga caccgaacag gaaaccgtcc tcaggcacct tcgattccag
     1081 gtaatcgagc accggcggca ggtcttcctg catcgcctgc gcgatgccct gcaggtcccg
     1141 cggctggccc cagatgccgg gttgcagcac tgccttgtcg aagatcttcc agacgatgat
     1201 gtccgcgacg cgcgtgtcgg catattcctc gagccagcgc gcctgggcac gatcggccac
     1261 ggcggccggc agcagtggat ggcgctccgg atagcgttcg tccaggtact gcaggatcac
     1321 gctcgagtcg ttcagcacga aaccgtcgtc gagcagcacc gggacccgcc gcagcgggct
     1381 cagctgcgaa aagcggtcgt caccgatata cggagtgatc ggatcgatgc ggtagccgac
     1441 gcctttgagt tcgagggcga cgagcgcctt gcgcacgaag ggggacacgt agctgccgat
     1501 gatctcgatc acggcggcgg acccctcaac cgcccgcagt gcccggagcc ttgccctcca
     1561 gctcggccag ccgctgctcg agcgcggtga gcttctcgcg cgtgcggcgc aggaccgcgc
     1621 tctgcacatc gaattcctcg cgtgtcacga ggtcgagccg cgcgagctgc gactgcagca
     1681 cgcccttgaa gttctgctcg aggtcgcgac gcagggcgac gaggttctcg ggaacgctgc
     1741 cggccaggcg ccgcgccagg tcgtcgatca tgcg
//
LOCUS       DBNHBY010000025         2619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_677885, whole genome shotgun sequence.
ACCESSION   DBNHBY010000025 DBNHBY010000000
VERSION     DBNHBY010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_677885"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC16HB_00655"
     CDS             complement(<1..1190)
                     /locus_tag="AC16HB_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605327.1"
                     /translation="MNFFEICIRRPVLSTVLSLLVLVIGLISYSRLAVREYPKIDEPV
                     VSVQTKYSGASAEVVESQITKVLEDSLAGIEGVELMTSASRSETSQINVRFRITRDPD
                     SAAADVRDKVARVRARLPDEVDEPVIAKVEADSFPIMWMAVTSGGRTALEVSDYLTRY
                     VKPRLSVLPGAADVWIYGERKISMRINVDRDRLAGYRLTPQDVEDALRRQNVELPSGR
                     IESAAREFSIVAATDVSSRGEFENLIVAHVSGYPVRLRDVADVRVGPVEERVIARFNG
                     ESSINLGMVKQATGNPLELSRATRAEIAKINATLPEGMKITVAYDASVFIDRSIESVF
                     RTVAEAVALVVLVIFFFLRNLRATLIPLVTIPLSLVGAFALMYAFGFTINTLTLLAMV
                     LAIGL"
     gene            complement(1245..2417)
                     /locus_tag="AC16HB_00660"
     CDS             complement(1245..2417)
                     /locus_tag="AC16HB_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5605328.1"
                     /translation="MIKHKVALWATAGLLAAVVVAYFAPRWLASEPPTLDAGAKAAAA
                     ASDTKAARDTGKSAAAGGPTPVEVADVRPSAVQEDLQAVGSLRSNESVVLRPEVAGRI
                     ARIGFRDGEAVRRGQLIVGLDASVNEAEVAQARAEYELAESNLRRTEDLARKNFISSS
                     AQEQAASNVQVSAAKLKLAEARLAKMKIVAPFDGVVGIRNVSVGDYVKDGTDLVNIED
                     VGTLKVDFRLPERNVAQIRGGQPVEVTADALPNETFRGALEAINPRVDAAGRSLEVRA
                     KLANPERKLRPGMFVRVRVIVGERANALLVPEEAIVPQGEDFYVFRVEEGNARRVKVK
                     TGVRRDAKVEIVEGLAAGDKVVTAGMRLVRDGQPVKIVAATAAGAGQADAADKAVK"
     gene            complement(2485..>2619)
                     /locus_tag="AC16HB_00665"
     CDS             complement(2485..>2619)
                     /locus_tag="AC16HB_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR5605329.1"
                     /translation="AHLASLHDIDWRWVKGHAGVPGNERADELANRGVAAARAAAEGA
                     "
BASE COUNT          404 a          957 c          838 g          420 t
ORIGIN      
        1 aggccgatcg ccagcaccat cgccagcagc gtcagcgtgt tgatcgtgaa gccgaacgcg
       61 tacatcagcg cgaacgcgcc caccagcgac agcgggatcg tcaccagcgg gatcagcgtg
      121 gcgcgcagat tgcgcaggaa gaagaagatc accagcacca cgagcgcgac cgcttcggcc
      181 acggtgcgga acaccgattc gatcgagcgg tcgatgaaca ccgacgcgtc gtacgcaacc
      241 gtgatcttca tgccctccgg cagcgtggcg ttgatcttcg cgatctcggc gcgcgtggcg
      301 cgcgacagct cgagcggatt gccggtcgcc tgcttgacca tgccgaggtt gatcgacgac
      361 tcgccgttga agcgcgcgat cacgcgctcc tcgaccgggc cgacgcgaac atcggcgacg
      421 tcgcgcagcc gcaccgggta gccggacacg tgcgcgacga tcaggttctc gaactcgccg
      481 cgcgaactga cgtcggtggc ggcgacgatc gagaactcgc gcgccgccga ttcgatcctg
      541 ccggacggca gctcgacgtt ctgccgccgc agcgcgtcct ccacgtcctg cggggtcagc
      601 cggtagcccg ccagccgatc gcggtcgacg ttgatgcgca tcgagatctt gcgctcgccg
      661 tagatccaca cgtcggccgc gcccggcagc accgacagcc gcggcttcac gtagcgcgtg
      721 aggtagtccg acacctccag cgccgtgcgt ccgccggaag tcacggccat ccacatgatc
      781 gggaacgaat cggcctcgac cttggcgatc accggttcgt cgacctcgtc cggcagccgg
      841 gcgcgcacgc gcgcgacctt gtcgcgcaca tcggccgcgg cggagtccgg gtcgcgcgtg
      901 atgcggaagc gcacgttgat ctggctggtc tcggagcgac tggccgaggt catcagttcc
      961 acgccctcga tgccggccag ggaatcctcg agcaccttgg tgatctgcga ctccaccact
     1021 tcggccgacg cgccggaata cttggtctgc acgctgacca ccggctcgtc gatcttcgga
     1081 tactcgcgca ccgccagccg cgaatacgag atcaggccga tcaccagcac cagcagcgac
     1141 agcacggtcg acaggaccgg gcggcggatg cagatttcga agaagttcat ggcctcgagc
     1201 gcggcgcgaa cggaatcatc ataggcccgc aagcgtgcga ggcgtcattt caccgccttg
     1261 tcggccgcgt ccgcttgccc cgctccggcc gccgttgctg cgacgatctt caccggctgc
     1321 ccgtcgcgca ccaaccgcat gccggcggtg acgaccttgt cgccggccgc gagcccttcg
     1381 acgatctcga ccttcgcgtc ccgccgcacg ccggtcttca ccttgacccg cctggcgttt
     1441 ccttcctcca cgcgaaacac gtagaagtcc tcgccctgcg ggacgatcgc ctcctccggc
     1501 acgagcagcg cgttggcgcg ttcaccgacg atcacgcgca cgcgcacgaa catgccgggt
     1561 cgcagcttgc gttccggatt ggcaagcttg gcgcgcacct cgagcgagcg accggccgcg
     1621 tcgacgcgcg gattgatcgc ctcgagcgcg ccgcggaaag tttcgttcgg cagcgcatcg
     1681 gccgtcactt cgaccggctg cccgccgcgg atctgcgcga catttcgctc cggcagccgg
     1741 aagtcgacct tcagcgtgcc gacgtcctcg atgttcacga ggtcggtgcc gtccttcacg
     1801 taatcgccga cgctgacgtt gcggataccg acgacgccgt cgaacggcgc cacgatcttc
     1861 atcttcgcca gccgcgcctc ggcaagcttc agcttcgccg cgctgacctg cacgttcgac
     1921 gccgcctgct cctgggcgct ggaactgatg aagttcttgc gcgccaggtc ctcggtgcgt
     1981 ctgagattcg actctgcgag ttcgtattcg gcgcgtgcct gcgcgacctc ggcttcgttg
     2041 acgctggcat cgagccccac gatcagctgg ccgcgccgca ctgcctcgcc gtcgcgaaag
     2101 ccaatccgcg cgatgcggcc ggcgacttcg ggccgcaaca ccacgctctc gttggatcgc
     2161 agcgatccga ccgcctgcag gtcttcctgc accgcgctcg gtcgcacgtc ggccacttcg
     2221 accggcgtcg gcccgccggc tgcagccgac ttgccggtgt ccctggccgc cttcgtgtcg
     2281 ctcgctgccg ctgcagcttt ggcgcccgcg tcgagcgtcg gcggctcgga cgcgagccag
     2341 cgcggcgcga agtaggcgac gacgacggcg gccagcaatc cggccgtcgc ccagagggcg
     2401 accttgtgct tgatcatcag ggaagcgcgg caacgaacgg cgccgggcgc cgcgcaccga
     2461 ctttagcacc cggcacgcgc agcgttacgc accttccgcc gcagcacgcg cggccgcgac
     2521 gccgcggttg gccagttcgt cggcgcgctc gttgccgggc actccggcat ggcccttgac
     2581 ccagcgccag tcgatgtcgt gcaggctcgc aagatgcgc
//
LOCUS       DBNHBY010000026         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1828322, whole genome shotgun sequence.
ACCESSION   DBNHBY010000026 DBNHBY010000000
VERSION     DBNHBY010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1828322"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC16HB_00670"
     CDS             <1..214
                     /locus_tag="AC16HB_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR5605330.1"
                     /translation="GAGENRGETLANDHVVTQWSGPLASGAPMVQRWPAPDVSSDRLR
                     VVAVVQDAAHGQPLQAVALPLAACGK"
     gene            complement(266..1036)
                     /locus_tag="AC16HB_00675"
     CDS             complement(266..1036)
                     /locus_tag="AC16HB_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5605331.1"
                     /translation="MPRAIDVVEELKRELKARGLKYAEIARRIGMSEASVKRMFSERN
                     FTLARLDEILTAAGIDFTEIAHGTDREQHLISQLTEAQEREIVDDPKLFTVAVDALNL
                     LTFDDMLAAHELTAAELVAQLVKLDRMGVIGLLPNNRFRLRIARTFAWIPNGPIMTAF
                     KNNARDFFDSSFAGDDEIMMLLNGRLSRASSAALVERLKRLAREFSEQHIGDARLPAE
                     QRPPISLLLACRPWLPQFMRELARKPGPQTLPTRVVRR"
     gene            complement(1234..>2009)
                     /locus_tag="AC16HB_00680"
     CDS             complement(1234..>2009)
                     /locus_tag="AC16HB_00680"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5605332.1"
                     /translation="DRGRFDSPDVQHVLIEAMKLAFADVYTYVADPRAMRVTPAQMLD
                     PAYLASRARLIDPQRAQAFQSGSLPTGGTIYLSAADSSGMMISFIQSNYMGFGSGVVV
                     PGYGISLQNRGHGFAFDPGHANCVAPGKRPFHTIIPGFLMNEGRPQMSFGVMGGNIQP
                     QGHVQTLTRILLANQQPQAACDAPRWKWDSGLKIDVEHTMPPAVRADLARRGHQIADL
                     TDSYMDFGSGQFIWRLGDPAVDGYVAASDSRRDGQAVGY"
BASE COUNT          318 a          679 c          697 g          315 t
ORIGIN      
        1 cggcgccggc gagaaccgcg gcgagacgct ggccaacgat catgtcgtca cgcaatggag
       61 cgggccgctg gcgtccggcg ccccgatggt gcagcgctgg cccgcgccgg acgtgtcgag
      121 cgaccggctg cgcgtcgttg ccgtcgtgca ggacgccgcg cacggtcagc cgctgcaggc
      181 ggtcgcgctg ccgctggcgg cctgcggcaa gtaaggactg cgagcgcgag gcggcgcccg
      241 acgcgcagcc gcaccttgcg ttggttcagc gccgcaccac gcgcgtcggc agcgtttgcg
      301 gcccgggctt gcgcgccagc tcgcgcatga attgcggcag ccacggccgg catgcaagca
      361 gcaggcttat cggcggacgc tgctcggcgg gaagccgggc atcgccgatg tgctgctcgg
      421 agaactcgcg tgccaaccgc ttcaggcgct cgaccagcgc ggcgctcgac gcgcgcgaga
      481 gccgcccatt cagcagcatc atgatctcgt cgtcgccggc gaagctcgaa tcgaagaaat
      541 cgcgcgcgtt gttcttgaac gcggtcatga tcggcccgtt cggaatccac gcgaacgtgc
      601 gcgcgatccg cagccggaac cgattgttcg gcagcagccc gatcacgccc atgcggtcga
      661 gcttgaccag ttgcgcgacc agttcggccg ccgtcagttc atgcgcggcc agcatgtcat
      721 cgaacgtgag caggttcagc gcgtccaccg cgacggtgaa gagcttcgga tcgtcgacga
      781 tctcgcgctc ctgcgcctcg gtcaactgcg aaatcaggtg ctgctcgcgg tcggtgccgt
      841 gcgcgatctc ggtgaagtcg atgccggccg cggtcagaat ctcgtccagc cgcgccagag
      901 tgaaattgcg ctctgagaac atccgcttca cgctcgcctc ggacatgccg atgcggcgcg
      961 cgatttcggc gtacttcagc ccgcgcgcct tcagctcgcg cttcagttcc tcgacgacgt
     1021 cgatcgctcg cggcacggct gcctcccctg cggacgggcc gcagtatcgt ggatcgcggc
     1081 cggcggcgac aagctctgac gctacggctt gcgcgtgcgt gtgaaagacg cctcccgcgc
     1141 ggtcggcgca gcgacggcgg gccgcgcgac gaacccgctt acgaagggag cgacgcggca
     1201 cggcgccgcg accgagcaac gaaccggcgc ggctcagtaa ccgacggcct gtccgtcgcg
     1261 gcgcgaatcg ctggcggcca cgtagccatc gaccgcaggg tcgcccagtc gccagatgaa
     1321 ctggccactg ccgaagtcca tgtagctgtc ggtcaggtcg gcgatctggt gcccacggcg
     1381 ggccagatct gcgcgcaccg ctggcggcat cgtgtgctcg acatcgatct tcaggcccga
     1441 atcccacttc cagcgcggtg cgtcgcaagc cgcctgtggc tgctggttgg cgagcaggat
     1501 gcgcgtgagc gtctgcacgt gtccctgggg ctggatgttg ccgcccatca cgccgaagct
     1561 catctgcggc cgcccttcgt tcatcaggaa gccggggatg atcgtgtgga atggccgctt
     1621 gcccggcgcc acgcagttcg catgtccggg atcgaacgcg aacccgtgac cgcggttctg
     1681 cagcgagatg ccatagcccg ggacgacgac cccggacccg aagcccatgt agttggactg
     1741 gatgaacgag atcatcatgc cgctcgaatc cgcggccgac agatagatgg tgccgccggt
     1801 gggcaagctg ccggattgga aggcctgcgc gcgctgcgga tcgatcagcc tggcgcggct
     1861 cgcgaggtag gcggggtcga gcatctgcgc gggcgtcaca cgcattgcac gcggatcggc
     1921 cacgtaggtg tagacatccg caaaggcgag cttcatcgcc tcgatcagca cgtgctgcac
     1981 gtcgggcgaa tcgaagcggc cgcggtcga
//
LOCUS       DBNHBY010000027         3409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_339727, whole genome shotgun sequence.
ACCESSION   DBNHBY010000027 DBNHBY010000000
VERSION     DBNHBY010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_339727"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="AC16HB_00685"
     CDS             complement(<1..463)
                     /locus_tag="AC16HB_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5605333.1"
                     /translation="MSNPVDALILDLLEWIGSDPKPYPEVIDAWRTSCPRLPVWEEAN
                     ERGFVDRQHRAGVGNFVAVSPRGAAFLAAQRPLPLKFATSHLRDAEFRTEGLRPFFAY
                     RDLGLREATHGKAVAHVIRAVPGAEFEGKPHRHRTTFQFIYVLRGWIEFEYE"
     gene            complement(503..1237)
                     /locus_tag="AC16HB_00690"
     CDS             complement(503..1237)
                     /locus_tag="AC16HB_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5605334.1"
                     /translation="MQPTERTYLQDDEGLSVAATVVAVRDGAFACDRTCFYPGGGGQP
                     SDAGSARMAGGETLRVESAHADEDGVIWHVGPLQPDADLVGQSVLLAVDAARRRALSR
                     YHTVLHVLNTIALRDYGGWITGAQIAADYARIDFKLERLSPALCAELERKVNDVLQGD
                     HAIRAYSLTEREFAQRGDLLRTLEVRPPVVDGRVRVVEIAGFDAQACGGTHVNSTREL
                     GRLSIFRTENKGRINKRLYVRLDPAE"
     gene            complement(1243..2376)
                     /locus_tag="AC16HB_00695"
     CDS             complement(1243..2376)
                     /locus_tag="AC16HB_00695"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5605335.1"
                     /translation="MNAPETRISRRSNLDPRLLERTHPLPISWYFDPAWFEREMLLLF
                     NRGPNYVGHERMVPEIGDYKVLPWTENGKVLVRSEAGVNLLSNVCRHRQAQLLDRRGN
                     VQNIVCPAHRWTYDLNGKLLGAPDFDTTPQCNLPRTPLTSWQGLLFAGPRDPNVDLKD
                     FPLTADYDFSGYVFDRLQVDECPFNWKTFLEIFLELYHVEAVHPGLQRWVDAGNYEWG
                     FGDRWSYQILGIKDEFRSQTSPKYAKYRDTILAYSGGRLPKYGTVWSILYPNVMIEWY
                     PHSLVVSTLIPRSADHTTNIVEFYYPEEIKAFEPQIVEAHQAAYAESAAEDAEVCTRL
                     HKGRRALWVAGEDDVGPYHTPHEDGMIHLHDWIRREMGQRRTT"
     gene            complement(2473..2889)
                     /locus_tag="AC16HB_00700"
     CDS             complement(2473..2889)
                     /locus_tag="AC16HB_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR5605336.1"
                     /translation="MTLAVLRDRLHEWAEHLKRDALTLWFAYRHPQAPWSAKFLGAFV
                     VAYALSPIDLIPDFVPIFGYLDDVLLLPGLIWLALRLLPPAVLAASRARADCWLAERR
                     ARPRSMAGAVLVVALWLACLWLTWLFGRWAWALLQG"
     gene            complement(2927..>3409)
                     /locus_tag="AC16HB_00705"
     CDS             complement(2927..>3409)
                     /locus_tag="AC16HB_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5605337.1"
                     /translation="TKRKYTAGSVLLAIGRRGTPRKLGVPGEESAKVVYRLVDPEQYR
                     GKAVLVVGGGDSALEAALALAEQHGTEATLSYRGESFTRVKGKNRERLQQQQAARRVR
                     VLMPSQVKAILPDHVIIHCEGESKRYRNDAVIVCAGGELPIPLLQRIGIAFETKHGSA
                     "
BASE COUNT          540 a         1168 c         1141 g          560 t
ORIGIN      
        1 cctcgtattc gaactcgatc cagccgcgca ggacgtagat gaactggaac gtcgtgcggt
       61 gccggtgcgg cttgccttcg aactcggccc ccggcaccgc acggatcacg tgcgcaaccg
      121 ccttgccatg agtcgcttct cgcaacccga gatcgcgata ggcgaagaac ggtcgcaagc
      181 cttcggtcct gaattccgcg tcgcgcaggt ggctcgtcgc gaacttcagc ggcagcgggc
      241 gctgtgcggc aaggaaggcc gcgccgcgcg gcgacaccgc gacgaaattg ccgacgcccg
      301 cgcgatgctg gcgatcgaca aaaccgcgct cgttggcttc ctcccacacg ggcagccgcg
      361 ggcaggaggt gcgccacgcg tcgatcacct ccggataggg cttcggatcg gagccgatcc
      421 attccagcag gtccagaatc aacgcgtcca ccgggttcga catggcgcct cccgtgtcgg
      481 tgccgtccga ggagagcgca gcttactcgg ccggatcgag ccgcacgtac agacgcttgt
      541 tgatgcggcc cttgttctcg gtgcggaaga tcgacagccg cccgagttcg cgcgtcgaat
      601 tcacgtgggt gccgccgcag gcctgcgcgt cgaatcccgc gatctcgacc acccgaacgc
      661 gaccgtcgac gaccgggggc cgcacctcga gcgtacgcag caggtcgccg cgctgcgcga
      721 attcccgttc ggtcaggctg tatgcccgga tcgcgtggtc gccctgcagc acgtcgttga
      781 ccttgcgttc gagttctgcg cacagcgcag gcgacagccg ctcgagcttg aagtcgatac
      841 gcgcgtagtc ggccgcgatc tgcgcgccgg tgatccagcc gccgtagtcg cgcagcgcga
      901 tcgtgttcag cacatgcagc accgtgtggt agcgcgacag cgcacgtcgt cgcgcggcgt
      961 ccaccgccag aaggaccgat tggccgacca ggtctgcatc cggctgcagc ggaccgacat
     1021 gccagatcac cccgtcctcg tccgcgtgcg cggattcgac ccgcagggtt tcaccgcccg
     1081 ccatacgcgc cgagccggca tccgacggct gaccgccgcc gccgggatag aagcaggtgc
     1141 gatcgcacgc gaacgcgcca tcgcgcacgg cgacgaccgt ggcggcgacg ctcaggccct
     1201 cgtcgtcctg caggtaggtg cgctcggtcg gctgcacgct gactaagtgg tgcgccgctg
     1261 ccccatctcg cgccggatcc agtcgtgcag gtggatcatg ccgtcttcgt gcggcgtgtg
     1321 atacgggccg acatcgtctt cgccggcgac ccacagcgcg cggcgcccct tgtgcaggcg
     1381 cgtgcagacc tccgcatcct cggccgcgct ttccgcgtac gcggcctggt gcgcctcgac
     1441 gatctgcggc tcgaacgcct tgatctcttc cgggtagtag aactcgacga tgttggtcgt
     1501 gtgatcggcg ctcctcggga tcagcgtgct gacgacgagg ctgtgcggat accactcgat
     1561 catcacgttc gggtacagga tcgaccacac ggtgccgtac ttgggcaacc tgccgcccga
     1621 gtaagccagg atcgtgtcgc ggtacttggc gtacttgggc gatgtctgcg accggaactc
     1681 gtccttgatg cccaggatct ggtagctcca gcggtcgccg aagccccact cgtagttgcc
     1741 ggcatcgacc cagcgctgca gacccgggtg tacggcctcg acgtggtaga gctcgagaaa
     1801 gatctcgaga aacgtcttcc agttgaacgg gcattcgtcg acctggaggc ggtcgaacac
     1861 gtagccgctg aagtcgtaat cggccgtcag cgggaagtcc ttcaggtcca cgttcggatc
     1921 gcgcggaccg gcgaacagca gtccctgcca gctcgtgagc ggcgtgcgcg gcaggttgca
     1981 ttgcggcgtg gtgtcgaagt ccggcgcgcc gagcagcttg ccgttcagat cgtaggtcca
     2041 gcgatgcgcg gggcagacga tgttctgtac gttgccgcgg cggtcgagca gctgtgcctg
     2101 tcggtgccgg cacacgttcg acagcaggtt cacgccggct tcgctgcgca ccagcacctt
     2161 gccgttctcg gtccacggca gcaccttgta gtcgccgatc tcgggcacca tgcgctcgtg
     2221 accgacgtag ttcggaccgc gattgaacaa cagcagcatc tcgcgctcga accacgcggg
     2281 gtcgaagtac cagctgatcg gcagcggatg cgtgcgttcg agcagccgcg ggtcgagatt
     2341 cgagcgccgc gaaatccggg tctcgggtgc gttcatcggc tcttctcctg cgggtcgccc
     2401 tgctttcggc gcgcgatcat acgcgcgggt cacatcatca aacgccgcag cggtgcgcgg
     2461 cgctgacctc ggtcaaccct gcaggagtgc ccaggcccag cggccgaaga gccaggtcag
     2521 ccacaggcac gcgagccaga gcgcgaccac cagcaccgcg ccggccatgc tgcgtgggcg
     2581 cgcacgccgc tcggcgagcc agcagtcggc gcgtgcgcgg ctggcggcga ggacggcagg
     2641 gggcagcagg cggagcgcga gccagatcag cccgggcaac agcagcacgt cgtcgaggta
     2701 gccgaagatc ggcacgaagt ccgggatcag gtcgatcggc gacagtgcat acgcgaccac
     2761 gaacgcaccg aggaacttcg cgctccacgg cgcctgcgga tggcggtacg cgaaccagag
     2821 cgtcaatgca tcgcgcttca ggtgctcggc ccactcgtgc aggcggtcgc gcaggacggc
     2881 gagggtcatc gtgcttcggt ttccttgcgc gcaccggccg gcgtcgtcat gcgcttccgt
     2941 gcttggtctc gaacgcgatg ccgatccgct gcagcagcgg aatgggcaac tcgccgccgg
     3001 cacagacgat caccgcatcg ttgcgatagc gcttcgactc tccttcgcaa tggatgatga
     3061 cgtgatccgg caggatcgcc ttgacctgcg acggcatcag cacgcggacg cggcgcgccg
     3121 cctgctgctg ctgcaggcgc tcccggttct tgcccttcac gcgcgtgaag gattcgccgc
     3181 ggtacgacag cgtcgcctcg gtgccgtgct gctccgccag cgcgagcgcg gcttccagcg
     3241 cgctgtcgcc gccgccgacg accagcaccg ccttgccgcg gtactgctcg ggatcgacca
     3301 gccggtagac gaccttcgcg gactcctcgc ccggcacgcc gagcttgcgc ggcgtgccgc
     3361 gccggccgat cgccagcagc accgagccgg ccgtgtactt gcgcttggt
//
LOCUS       DBNHBY010000028         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_948797, whole genome shotgun sequence.
ACCESSION   DBNHBY010000028 DBNHBY010000000
VERSION     DBNHBY010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_948797"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="AC16HB_00710"
     CDS             complement(<1..768)
                     /locus_tag="AC16HB_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5605338.1"
                     /translation="MSWLLPRPWLRLLAPVVLAAAVALSGQGAALLVTEVLIMALFAS
                     SLNLLMSFGGMVSFGHAAYFGLGAYGLALTVAKLGLPVWAGVVAGPVLATIAALLFGS
                     LCVRLSQIYFAMLTLACSEITYTVLFQAYDYTGGDTGITSFMAPRFGLGPQAFALLVL
                     AIVVAALLLLWRVVNSSLGLAIQSVGQEPNRAAALGYAPRRVQLIAFVVAGNLAGIAG
                     MLFSVYQGNAFPDYAGLGFTVDALVMVVIGGLYSFAGG"
     gene            complement(765..1634)
                     /locus_tag="AC16HB_00715"
     CDS             complement(765..1634)
                     /locus_tag="AC16HB_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5605339.1"
                     /translation="MLAAQVLNGLMEGMMLFLIASGLTLIFGITRIVNFAHGSFYMLG
                     AFLTYTAMSHLWPGTVGGFALSVLLGALAVACLGALFETLVLRRIYGADDLLQLIITV
                     ALVLILRDTVRAIWGANNVSVPMPAELSGAVQLGDAFLPSFQLAVFGVGLLVVALMIA
                     VIRYTRAGILLRAATDDRGMVALLGINQARIFTSVFAVGSFLAGFAGGLAAPFGNVNY
                     LLDTSVIVMAFIVVVIGGLGNLYGALAAALAVGVIKGVGVLYFPRLSMVLIFLIMAAV
                     LMARSLGRAGVSK"
     gene            complement(1665..2879)
                     /locus_tag="AC16HB_00720"
     CDS             complement(1665..2879)
                     /locus_tag="AC16HB_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5605340.1"
                     /translation="MKPRQFVWTLALLASVATTGASAQEPIRIGYAADISGACGPLVD
                     GATKAVRMGVEELNKAGGLLGRKIELIERDTKTRPDEGAKEVRDLIVNQKVDLITGVC
                     SSAVLLAETAVSAEFKVPMYSAIANTQTATIEKWQPYYWQVVPNALMEAVAAAEFFAQ
                     RKEWKKVTPLAFDYEWGHTSTKAFSEHLKKLRPDIEITPMIPVKLGETNMSSYITAAL
                     AQKPDAVLGLVFGGGLINLVQQGKAYGLFQKSNLVTLTTVDFLQSIGSGIPAGGLYGF
                     ARAPFFALLENPRAKAFVAAYKTRYGKEPDDWAVLTYDGLMFYAEAVRHAKTTKGDAL
                     MKSVTSIKYEGLRGPSRVRALDGMMNAASWVGVVASDPAYPYAVIKNPLRVDGEKTLP
                     SEATVKSLRGGK"
BASE COUNT          567 a         1056 c         1020 g          465 t
ORIGIN      
        1 gccgccggcg aaactataca ggccgccgat gaccaccatc accagcgcgt cgacggtgaa
       61 gccgagaccg gcgtagtccg gaaaggcgtt gccctggtac acggaaaaca gcattccggc
      121 gatgccggcg aggttgccgg cgacgacgaa ggcgatcagc tgcacgcggc ggggggcgta
      181 gccaagcgcg gcggcccggt tcggttcctg gccgacgctc tggatcgcca gcccgagcga
      241 cgagttcacg acgcgccaca gcagcagcag ggcggccacg acgatggcca ggacgagcag
      301 ggcgaaggcc tgcgggccga gcccgaagcg cggggccatg aaactggtga tgccggtgtc
      361 gccgccggtg tagtcatagg cctggaacag caccgtgtat gtgatctcgc tgcaagccag
      421 cgtgagcatc gcgaaataga tctgcgacag tcgcacgcaa aggctgccga acagcagtgc
      481 ggcgatcgtt gccaacaccg gcccggcgac gacgccggcc cacaccggca ggccgagctt
      541 ggccacggtc agtgccagtc cgtacgcgcc gagtccgaaa tacgccgcgt ggccgaagga
      601 gaccatgccg ccgaagctca tcagcaggtt caggctggag gcaaacagcg ccatgatcag
      661 cacttcggtg acgagcagcg cagcgccctg gccggacagc gccaccgcgg cggcaagcac
      721 caccggcgcg agcagccgca gccacggccg gggcagcagc cagctcactt gctcaccccg
      781 gcacggccga gcgagcgcgc catcaggacg gcggccatga tcaggaagat cagcaccatc
      841 gacaggcgcg ggaagtacag cacgccgacg cccttgatca cgcccactgc cagcgcggcg
      901 gccagcgcgc cgtacaggtt acccaggccg ccgatcacca ccacgatgaa ggccatcacg
      961 atgacgctgg tgtcgagcag gtagttgacg ttgccgaacg gcgccgccag cccgccggca
     1021 aagccggcga ggaacgagcc gaccgcgaac accgacgtga agatgcgcgc ctggttgatg
     1081 cccagcagcg ccaccatgcc gcggtcgtcg gtggcggcac gcagcaggat gccggcacgc
     1141 gtgtagcgga tcaccgcgat catcagcgcg acgaccagca ggccgacgcc gaacaccgcg
     1201 agctggaacg agggcaggaa ggcgtcgccg agctgcaccg cgccggacag ctcggccggc
     1261 atcggcaccg acacgttgtt cgcgccccag atcgccctaa cggtgtcgcg caggatcagc
     1321 accagcgcga cggtgatgat cagctgcagc agatcgtcgg cgccgtagat gcggcgcagg
     1381 accagcgtct cgaacagtgc gcccaggcag gccaccgcca gcgcccccag cagcaccgac
     1441 aaagcgaacc cgccgacggt gccgggccac aggtgcgaca tcgcggtgta cgtcaggaac
     1501 gcgcccagca tgtagaagga gccgtgcgcg aaattgacga tccgggtgat cccgaagatc
     1561 agcgtcaggc cggaggcgat gaggaacagc atcatcccct ccatcagccc gttcaggacc
     1621 tgggcggcga gcatcggctg tatccgtccg cgtggccgga gatcctactt tccgccgcgc
     1681 agcgacttga ccgtcgcttc gctcggcagc gtcttttcgc catcgacgcg cagcgggttc
     1741 ttgatgaccg cgtacgggta ggctggatcc gacgcgacga cgccgaccca ggacgccgca
     1801 ttcatcatgc cgtcgagcgc gcgcacgcgg ctcggtccgc gcaggccctc gtacttgatc
     1861 gaggtgaccg acttcatcag ggcgtcgccc ttggtcgtct tggcgtggcg caccgcctcg
     1921 gcgtagaaca tgaggccgtc gtaggtgagc accgcccagt catccggttc cttgccatag
     1981 cgtgtcttgt aggcggcgac gaaggccttg gctctggggt tttccagcaa cgcgaagaac
     2041 ggcgcgcgcg cgaacccgta caggccgccc gccggaatgc cactgcctat cgactgcagg
     2101 aagtcgaccg tggtcagcgt caccaggttg gacttctgga acaagccgta cgccttgccc
     2161 tgctggacca ggttgatcag gccgccgccg aacaccaggc cgagtaccgc gtcgggcttt
     2221 tgcgccagcg cggcagtgat gtacgaactc atgttggtct cgccgagctt caccgggatc
     2281 atcggagtga tctcgatgtc cggccgcagc ttcttcaggt gctcgctgaa ggccttggtc
     2341 gacgtgtggc cccactcgta atcgaacgcc agcggcgtga ccttcttcca ctccttgcgc
     2401 tgggcgaaga actcggccgc ggcgaccgcc tccatcagcg cgttgggcac gacctgccag
     2461 tagtacggct gccacttctc gatggtcgcc gtctgcgtat tggcgatcgc cgagtacatc
     2521 ggcaccttga actctgcgga gacagccgtc tcggccagca gcaccgcgga cgagcacacg
     2581 ccggtgatca ggtcgacctt ctggttgacg atcaggtcgc gcacttcttt cgcgccctcg
     2641 tccggccgcg tcttggtgtc gcgctcgatg agttcgatct tgcgcccgag caggccgccc
     2701 gccttgttca gttcctcgac gcccatcctg actgccttgg tggcgccgtc gaccagcggc
     2761 ccgcaggcgc cggatatgtc ggcggcgtag ccgattcgga tcggctcctg cgccgatgcg
     2821 ccggtcgtgg caacgctcgc cagcagcgcg agtgtccata cgaactgtct tggtttcatc
     2881 ccctctctcc tgtctgcttt ggccaatggt ccggatggat tccggaccga ttacgtatcg
     2941 cgcacactct cgctgccggc aacgggcctg gcagcggggg ccgaacgccc tacactggcg
     3001 tcggtcccga ttgagtgaaa caacatatcg accgcccttg cgttgcagtg gcgtcaaggc
     3061 ggatcagccg gccttgacgg ccaggaccgg gcaggaaacc gtcagcaa
//
LOCUS       DBNHBY010000029         5917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_68765, whole genome shotgun sequence.
ACCESSION   DBNHBY010000029 DBNHBY010000000
VERSION     DBNHBY010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_68765"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..863)
                     /gene="rraA"
                     /locus_tag="AC16HB_00725"
     CDS             complement(357..863)
                     /gene="rraA"
                     /locus_tag="AC16HB_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HIR5605341.1"
                     /translation="MESTPFATTDLCDANEGRFASGELRLLPPGWLAFGRRVRYHGSV
                     VTLKLFEDNSLLAETVKTHGAGKVLVVDAGGSTRCAVLGGNLAAAAAKNQWQGMIVFG
                     AVRDADEIDACDLGVRALALIPRRSVKRGEGQRDVAIEIQGVPVRPGDCVYVDRDGIL
                     VSAHPLLP"
     gene            complement(871..2127)
                     /locus_tag="AC16HB_00730"
     CDS             complement(871..2127)
                     /locus_tag="AC16HB_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605342.1"
                     /translation="MSTVAEATDPRQDVEVITLVGVAHGTSHFFHLLLPSLFPWLMKE
                     FDLSFTQAGFLTTVFFVVSGIGQALAGFVVDRVGARRVLMFGIGMLSVAGVLVGLATS
                     YAGLVLGAAAAGLGNSIFHPADFTLLNRRVTASRLGHAFSAHGLAGYLGWAAAPVFVT
                     GLAGVWNWHIAAFGAAAVGAIVFVLLQARQQIIDDQAAHAIIAATSTAAAGGRAQSQF
                     AFLGSAAVWLCFLFFFLTTSGFGILQTYAPAVLGNVYAMPLALATAGLTAYLLGSAAG
                     ILTGGFVAARFPDSDKVIALMLGLAALVALVLATATVPRWAVLACMATMGFGVGVAGP
                     NRDLLVRKAATSQFGKRSYGRVYGFVYSGTDVGLAMAPMLLGPVLDAGHFAVALYAVA
                     AFQVGALIGALRVGVRTRSTAIAAAE"
     gene            complement(2138..3319)
                     /locus_tag="AC16HB_00735"
     CDS             complement(2138..3319)
                     /locus_tag="AC16HB_00735"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E22 family MetX-like putative esterase"
                     /protein_id="HIR5605343.1"
                     /translation="MLWTRQAALTAIVVLVCALAGAAHADVGIVEKKVFTLPAYTTVG
                     GQTIKSVRIGYETYGQLNARGDNAIFISHFYSGTSHAAGKYKESDVAPGYWNSIIGKG
                     RAIDTDKYFVVSADTLVNLNVRDPNVTTTGPATLDPDTGKPYGMSFPVVAMRDSVRVH
                     KALLDSLGVKRLVAAAGASGGSIQAMEWGALYPDFVERVVHAIGPGFDIHPYVVGLLD
                     VWALPVKLDPKWNNGNYYGREEPVEGVAQALKIVTLTARHFGWAEKTFGYKWSDERKN
                     PADAVGNTFQIESALNAAGGARAKTTDANHLIYMSKANQLYRLTDAEVKGIKAKILFV
                     PAASDLIFPPELSRRALERFRAQGGRGELFVIDGDGGHLDGVLAVAKAAEAIRVFIES
                     K"
     gene            3396..4577
                     /locus_tag="AC16HB_00740"
     CDS             3396..4577
                     /locus_tag="AC16HB_00740"
                     /inference="COORDINATES: protein motif:HMM:NF022514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HIR5605344.1"
                     /translation="MRRTRYISRARHSADAETLAKLSRRLAESGSRIEDRLWERRLAE
                     LINDRLTRGFADAIESALEDLGRTNARAYDDLADLAESSAESAAIDIDGKPHDALLLA
                     VPVLAWSRYKLPTTTLSSALVDNVAVQITGHLAARGTRLAVADYLYSIDQLPESLGEV
                     YDCAHGLFEAATQGKQMKVDPKSLREPVSMLADTRYVLAALIAPQGQALYHWQETGVD
                     PDAKQGAITTFCEQLGSVLQPLMTGCRYRVLPPNAFHAALRDADRQLREFSVEAAASY
                     LKVAYDIKPGELQATVAAFEDKRVEEMRIGISKAGDDDAVIEGVVWPLLGDEEEKAQE
                     EIEATLRVQGIARITTHAQRFPLEYCDDCGAPMFPNPAGHAVHAEPPEEADEQPSAPL
                     H"
     gene            complement(4589..5503)
                     /gene="rocF"
                     /locus_tag="AC16HB_00745"
     CDS             complement(4589..5503)
                     /gene="rocF"
                     /locus_tag="AC16HB_00745"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HIR5605345.1"
                     /translation="MNHRVSLIGAPTDFGASVRGASMGPEALRVAGIGAALQSHGAEV
                     IDRGNLSGPANPWLPPVDGYRHLAEVAAWSHLVHDAVLDELRADRLPVVLGGDHSLAI
                     GSIAAVARHCREKRRSLRVLWLDAHADFNTNVLTPSGNLHGMPAACLCGHGPRELVSI
                     SGATPAISSKSIRQIGIRSVDPGEKRLVHEAGLEVFDMRFIDEMGLRHTMELALAMMN
                     GDTHLHVSFDVDFLDPEIAPGVATTVRGGPGYREAQLCMEMIADSGLLASLDVMELNP
                     ALDVRNRTAELAVDLIESLFGKSTLMRT"
     gene            5575..>5917
                     /locus_tag="AC16HB_00750"
     CDS             5575..>5917
                     /locus_tag="AC16HB_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5605346.1"
                     /translation="MSGPIDHHRRTVALALLVAPLVHAQTGWPDRPIRLVVPYPPGGL
                     TDTLARSIAEPLSKALGQPVIVDNKPGAGTLVGAQTVARSASDGYTLLMATSTTLGIS
                     PALYRNNMIDPV"
BASE COUNT         1057 a         2061 c         1841 g          958 t
ORIGIN      
        1 ccttccagcg cgggctttcg ttccactcct tctgcaggcg ttgcgcctca agcttgcgtg
       61 cggtcatgcg tgctcctgtg agaaatagcg ttcaaccttt gcgtcgatcc gggtgcttcc
      121 accggccgtg gcccgcgtag cactggaacg aaatgctagg cgctgacgcg cagcgcagca
      181 acacaactat cttatagaag agttcttatt gaactcatat ttatcaatcg cttacaatca
      241 ttgtaccgcg atacggaatg acattcgcgg tcgtgaaagg aaggcttttg ccgcgcacaa
      301 gtcttcattc cacgattcga aatcgttgtc gcgttttgaa aaagtcatcg gccaattcac
      361 ggcagcagag gatgcgcact gacaaggatg ccgtcgcgat cgacgtagac gcaatcgccc
      421 ggtcgcaccg gcacgccctg aatctcgatg gcgacatcgc gctgcccctc gccgcgcttg
      481 acggagcggc gcggaatcaa agccagcgca cgcacgccga gatcacaggc atcgatctcg
      541 tccgcgtcgc gcaccgcgcc gaacacgatc atgccctgcc actgattctt cgccgctgcc
      601 gccgcgaggt tgccgccgag cacggcgcag cgagtgctgc cgcccgcgtc gacgaccagc
      661 accttgcccg cgccgtgtgt cttgaccgtc tctgccagca gcgagttgtc ctcgaacagt
      721 ttcagcgtga ccacgctgcc gtgatagcgc acgcgtcgtc cgaaggcgag ccagccgggc
      781 ggcagcaggc gcagttcgcc gctggcgaag cggccttcgt tcgcgtcgca cagatcggtc
      841 gtggcaaatg gcgtcgattc catgtggtcc tcactcggcc gccgcaatcg cagtcgatcg
      901 ggttcgcacg ccgacccgca gcgcgccgat gagtgcgccg acctggaatg cggcgaccgc
      961 gtacagggcc accgcaaagt gccccgcatc gagcaccggc cccagcagca tcggcgccat
     1021 cgccaaaccc acgtcggtcc ccgaatacac gaagccgtag acgcgcccgt acgaccgctt
     1081 gccgaactga gatgtcgccg ccttgcgcac cagcaggtcg cgattggggc cggccacgcc
     1141 gacgccgaat cccatcgtcg ccatgcaggc gagcacggcc cagcgcggca ccgttgcggt
     1201 cgccagtacc agcgccacca gtgccgcgag accgagcatc agcgcgatca ccttgtcgct
     1261 gtcgggaaag cgcgccgcga cgaatccgcc ggtcaaaatg ccggccgcgc tgccgagcag
     1321 gtacgccgtc agacccgccg tcgcgagcgc aagcggcatt gcgtagacgt tgccgagcac
     1381 cgccggcgcg taggtctgaa gaatgccgaa gcccgacgtg gtcaggaaga agaacaggaa
     1441 gcacagccac acggccgccg aaccgagaaa cgcgaactgc gactgcgcgc gcccgcctgc
     1501 agcggccgtc gaggtcgccg cgatgatcgc gtgtgcagcc tggtcgtcga tgatctgctg
     1561 ccgcgcctgc aacaacacga acacgatcgc gccgaccgcg gccgcgccga acgccgcgat
     1621 gtgccagttc cacacgccgg cgagtccggt gacgaatacc ggcgccgcgg cccacccgag
     1681 ataccccgcc agcccgtgcg cggagaaggc gtgccccagc cgcgacgcgg tcacgcggcg
     1741 attcagaagc gtgaagtcgg ccgggtgaaa gatcgaattt ccgagccccg ctgctgccgc
     1801 cccgagcacg agccccgcgt agctcgtggc cagtccgacc agcacgccgg cgacactcag
     1861 catgccgatg ccgaacatca gcacgcgtcg cgcgccgacc cgatcgacga cgaatcccgc
     1921 cagcgcctga ccgatgccgc tgacgacgaa gaacaccgtg gtcaggaatc cggcctgcgt
     1981 gaaagacagg tcgaattcct tcatcagcca ggggaacagc gacggcagca gcaggtggaa
     2041 gaagtgcgac gtgccgtgcg cgacaccgac cagcgtgatc acctccacgt cttggcgcgg
     2101 gtcggtcgct tcggccacag tgctcacgcg ccgcctctca tttgctttcg atgaacacgc
     2161 ggatcgcttc ggccgccttc gccaccgcga gtacgccgtc gagatggccg ccgtcgccgt
     2221 cgatcacgaa cagttcaccg cgcccaccct gcgcgcgaaa acgctccagc gcgcgccggg
     2281 atagctccgg cgggaagatc agatcgctcg cggccggcac gaacaggatc tttgccttga
     2341 tgcccttgac ctcggcatcg gtcagccggt acagctggtt cgccttcgac atgtagatca
     2401 ggtgattggc atcggtcgtc ttcgcgcgtg cgccgcccgc agcattgagt gccgattcga
     2461 tctggaacgt attgccgacc gcatcggccg gattcttcct ttcgtcgctc cacttgtagc
     2521 cgaacgtctt ctcggcccag ccgaagtggc gcgcggtcag cgtgacgatc ttcaacgcct
     2581 gcgccacgcc ttcgaccggt tcctcgcggc cgtagtaatt gccgttgttc catttcggat
     2641 cgagcttgac cggcagcgcc cacacgtcga gcaggccgac cacgtatggg tggatgtcga
     2701 accccggccc gatcgcgtgg actacgcgct cgacaaaatc cggatagagc gcgccccact
     2761 ccatcgcctg gatcgatccg cccgacgcgc cggccgccgc caccagcctc ttcacgccga
     2821 gcgaatccag cagcgccttg tgcacgcgca ccgaatcgcg catcgcgacc accggaaagc
     2881 tcatgccgta tggcttgccc gtgtccggat cgagggtcgc cggccccgtg gtcgtgacgt
     2941 tcgggtctct gacgttcaga ttgaccagcg tgtcggcgct gacgacgaag tacttgtcgg
     3001 tgtcgatcgc cctgcccttg ccgatgatcg aattccagta gcccggcgca acgtcacttt
     3061 ccttgtactt gcccgccgca tgcgacgtcc ccgagtagaa gtgcgagata aagatggcgt
     3121 tgtcgccgcg cgcattcaac tgaccgtagg tctcgtagcc gatacgcacc gacttgatcg
     3181 tctgaccgcc gaccgtcgtg taagccggca gagtgaatac cttcttctct acgatgccga
     3241 cgtccgcgtg cgcagcacca gccagcgcgc acaccagaac cacgatcgcc gtcagcgcgg
     3301 cctgtctggt ccacagcatc gattccccct cgtcattcgt catggcgttc gacggcgagt
     3361 gggataatac ggcctgacct cacgaggaat tgcccatgcg ccgcacccgg tatatctccc
     3421 gcgcgcgcca ctcggccgac gccgaaacgc tggccaaact gtcccgccgc ctcgccgaat
     3481 ccggcagccg catcgaagac cggctgtggg aacggcgcct cgccgaactg atcaacgacc
     3541 gcctaacgcg cggcttcgcc gatgcgatcg agtcggcgct ggaggatctc gggcgaacca
     3601 atgcgcgcgc ttacgacgat cttgccgatc tcgccgagtc atctgccgag agcgcggcca
     3661 tcgacatcga tggcaagccg cacgacgcat tgctgctggc ggtgccggtg ctcgcatggt
     3721 cgcgttacaa gctgcccacg accacgctgt cgagcgcact tgtcgacaac gtcgcggtgc
     3781 agatcaccgg ccatctggcc gcgcgcggca cgcggctggc agtggccgat tatctgtatt
     3841 cgatcgacca attgcccgag tcgctcggcg aggtgtacga ctgcgcgcac gggctgttcg
     3901 aagcagccac gcagggcaag cagatgaagg tcgacccgaa gagcctgcgc gaaccggtgt
     3961 cgatgctggc cgacacgagg tacgtgctcg ccgcgctgat cgcgccgcag ggccaggccc
     4021 tctaccactg gcaggagacc ggcgtcgacc ccgatgccaa gcagggtgcg atcacgacgt
     4081 tctgcgaaca gttgggcagc gtgctgcagc ccctgatgac gggctgccgc taccgtgttc
     4141 tgccgccgaa tgcgttccac gcggcactgc gcgacgccga ccgccagttg cgggagttct
     4201 cggtcgaggc cgcggcgtcg tatctgaaag tcgcctatga catcaagcca ggagaactgc
     4261 aggcgacggt cgccgcgttc gaggacaagc gcgtcgagga gatgcgcatc ggcatctcca
     4321 aggcgggtga cgacgatgcg gtgatcgagg gtgtcgtgtg gccgctgctc ggcgacgagg
     4381 aggaaaaggc gcaggaggag atcgaggcca ccctgcgcgt gcaaggcata gcgcgcatca
     4441 ccacgcacgc gcagcgcttt ccgctggagt actgcgacga ttgcggcgcc ccgatgttcc
     4501 cgaaccccgc cggccatgcg gtgcacgcgg agccgcccga ggaagccgac gagcaaccct
     4561 cggcccccct gcattgacgc gcgccccttc aggtccgcat caaagtactc ttgccgaaaa
     4621 ggctttcgat cagatcgacc gcgagctcgg cggtgcgatt gcgcacgtcg agcgccgggt
     4681 tcaattccat cacgtcgagc gacgccagca gtcccgagtc ggcgatcatc tccatgcaca
     4741 gctgcgcttc gcggtaaccc ggtccgccgc gcacggtggt cgccaccccg ggtgcgattt
     4801 ccggatcgag gaaatcgacg tcgaagctca cgtgcaggtg ggtgtcgccg ttcatcatcg
     4861 ccagcgccag ctccatcgtg tggcgcagtc ccatttcgtc gatgaagcgc atgtcgaaca
     4921 cttcgagacc ggcctcgtgc acgaggcgct tctcgccggg atcgacgctg cgaatgccga
     4981 tctgccggat tgacttggag ctgatcgccg gcgtcgcgcc gctgatcgat accagttcgc
     5041 gcgggccgtg cccgcacaaa cacgccgccg gcatgccgtg caggttgccg ctcggcgtca
     5101 gcacgttggt gttgaagtcc gcgtgcgcgt cgagccacag cacgcgcagg ctgcgccgct
     5161 tctcgcgaca atgtctcgcc accgctgcga tcgagccgat cgcaaggctg tgatcaccgc
     5221 caagcaccac cggcagccgg tcggcgcgca gctcgtcgag caccgcgtcg tgcacgaggt
     5281 ggctccacgc cgcaacttct gccagatgcc ggtatccgtc cactggcggt agccacggat
     5341 ttgccggtcc cgaaagattg ccgcggtcga tcacctcggc gccgtggctt tgaagcgcag
     5401 cgccgatgcc ggccacgcgc aacgcttcgg gccccatgct cgcgccgcgc acgctggcgc
     5461 cgaagtccgt cggcgcgcca atcagcgaga ctctgtggtt catggcgtgg attctccacg
     5521 aagtcgcgct gcgcgggcag tctcggcgac aatgcgcgcc tcaggggaga ccggatgtcc
     5581 ggaccgatcg atcatcacag gcgcaccgtc gcactggcgc tgctggtcgc tccgctcgtc
     5641 cacgcgcaga cgggctggcc cgacaggccg atccggctgg tcgtgcccta cccgccgggc
     5701 ggactgaccg acacgctcgc gcgctccata gccgagccgc tttcgaaggc tctcggccag
     5761 ccggtgatcg tcgacaacaa gcccggcgca ggcacgctgg tcggcgcgca gaccgtggca
     5821 cgcagcgcga gcgacggata cacgttgctg atggcaacca gcacgacgct ggggatcagt
     5881 ccagccctct accgcaacaa catgatcgac ccggtcc
//
LOCUS       DBNHBY010000030         2237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1625484, whole genome shotgun sequence.
ACCESSION   DBNHBY010000030 DBNHBY010000000
VERSION     DBNHBY010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1625484"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16HB_00755"
     CDS             complement(<1..320)
                     /locus_tag="AC16HB_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR5605347.1"
                     /translation="MRKTVLSVALALAFPAAFAQGGPTQSGVELYGIVDIGVESIDNG
                     SGASATKITSGISTGSRWGIRGNEDLGGGYRALFTLENRFETDTGTLSNRGPIFFCGT
                     TCPGV"
     gene            472..1101
                     /gene="coq7"
                     /locus_tag="AC16HB_00760"
     CDS             472..1101
                     /gene="coq7"
                     /locus_tag="AC16HB_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR5605348.1"
                     /translation="MRDLLDEILGATDRALRTLAGAATAARAYPAERAATAELSDEQR
                     REAAALMRVNHVGEICAQALYEAQAIGTADSGLRAAFRSAAQEEADHLAWTRRRIVEL
                     GGRTSLLNPLWYAGSFGIGLAAARFGDKVSLGFMAETERQVEQHLQGHLARLPAQDAA
                     SRAVVAQMEADETRHGETARQMGGIELPWPARIAMKLAARVMTTTAYRL"
     gene            complement(1110..1529)
                     /locus_tag="AC16HB_00765"
     CDS             complement(1110..1529)
                     /locus_tag="AC16HB_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5605349.1"
                     /translation="MECTVRWIDGMSFVAETGSGHLVTMDGAPDGGGRNLAPRPMEMV
                     LLGTGGCTAFDVVLILKRGRHAVSGCEVKLEAERASEAPKVFTRIKFHFIVRGRGLQR
                     DAVERAVRLSHEKYCSASVMLAKTAALEYSIEVVETP"
     gene            1713..2141
                     /gene="rplM"
                     /locus_tag="AC16HB_00770"
     CDS             1713..2141
                     /gene="rplM"
                     /locus_tag="AC16HB_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5605350.1"
                     /translation="MKTFSAKPHEVRRDWYLVDASEKVLGRLAAEIARRLRGKHKPEY
                     TPHVDTGDFIVVVNASKIRVTGAKAADKTYYRHSGHPGGVYETSFEKMQNRFPGRVLQ
                     IAVKGMLPKGPLGYAMIKKLKIYADGAHPHTAQQPKALEI"
     gene            2149..>2237
                     /gene="rpsI"
                     /locus_tag="AC16HB_00775"
     CDS             2149..>2237
                     /gene="rpsI"
                     /locus_tag="AC16HB_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR5605351.1"
                     /translation="MIGSYNYGTGRRKSSVARVFIKRGTGKITV"
BASE COUNT          392 a          738 c          753 g          354 t
ORIGIN      
        1 acgcccgggc aggtcgtgcc gcagaagaag atcgggccgc gattgctgag cgtgccggtg
       61 tccgtttcga agcggttttc cagtgtgaac agcgcccggt agccgccgcc caggtcctcg
      121 ttgccccgaa tcccccagcg cgatccggtc gagatgccgc tcgtgatctt cgtcgccgac
      181 gcgcccgacc cgttgtcgat gctctcgaca ccgatgtcca cgatgccgta cagctcgaca
      241 ccggactgag tcggcccgcc ctgggcgaat gctgcgggga atgcgagtgc cagcgcgacg
      301 ctcagcacag tcttgcgcat tgtgtctctc ctctacgtgt ttgctgcgaa tttcgctgaa
      361 tactagctcc ggagatttcg gatgcgccgt ggcttcacgc aatgccgcgc gcgaaagtcg
      421 gtgcgcgtac catttccgca acacgacgtg cagcttcgaa gaggatgcga gatgagagat
      481 ctgctggatg aaatcctggg cgcgaccgat cgggcgctca gaaccttggc cggcgcggca
      541 actgcggccc gggcttatcc ggcggaacgg gccgcgacgg cagaactttc cgacgaacag
      601 cggcgcgagg cggcggcgct gatgcgggtg aaccacgtcg gagagatctg cgcgcaggcg
      661 ctgtacgaag cgcaggcaat cggcacggcg gactccggtt tgcgcgcggc cttccggtcg
      721 gccgcgcaag aagaggccga ccacctggca tggacgcggc ggcggatcgt ggaactgggc
      781 ggccgcacca gcctgctgaa tccgctctgg tacgcgggct cgttcggcat tggcctggca
      841 gcggcgcggt tcggcgacaa ggtgagcctg ggattcatgg cggagaccga gcgccaggtc
      901 gaacagcacc tgcaggggca cctggcgcgc ctgccggcgc aggatgcggc ttcgcgggcg
      961 gtcgtcgcgc agatggaggc ggacgagacg cgccacgggg agaccgcgcg gcagatgggc
     1021 ggcatcgaac ttccctggcc ggcccgcatc gcgatgaagc ttgccgcgcg cgtgatgacg
     1081 accaccgcgt accggctctg acccgatcgc tacggcgttt cgacgacttc gatcgagtat
     1141 tcgagggcgg ccgtcttcgc gagcatcacg gaggccgagc agtacttctc gtgcgaaagc
     1201 ctgaccgcgc gctcgacggc gtcgcgctgc agaccgcggc cgcgcacgat gaaatgaaac
     1261 ttgatccggg tgaagacctt gggcgcttcg ctggcgcgct ccgcctcgag tttcacttcg
     1321 caccccgaca ccgcgtggcg gccgcgcttc aggatcagga cgacgtcgaa tgcggtgcag
     1381 ccgccggtgc cgagcaggac catctccatc ggccgcggcg ccagattccg gccaccgccg
     1441 tcgggcgccc catccatcgt gaccaggtgg ccgctgccgg tttcggccac gaaactcatc
     1501 ccatcgatcc agcgaaccgt gcattccatg gctgtgcccg tgtcgagacg ctccgcgcgg
     1561 gagccggcgg tcgtttgcga aaagcacgcc cgcgcggata caatacgcgg cttttcgcgg
     1621 ctggcggctt gcgctgcccg tccgccagcg tcggctgtgc tgccgctctc ggtgccgcga
     1681 cgcgattatc cggttcgacg tcaagctaaa tcatgaaaac cttctccgcc aagccgcatg
     1741 aagtgcggcg cgactggtat ctggtcgatg ccagcgaaaa agtgctcggc cgcctcgcgg
     1801 ccgagatcgc gcgccgtctg cgcggcaagc acaagcccga gtacacgccg cacgtcgaca
     1861 ccggcgattt catcgtcgtc gtcaacgcgt cgaagatccg ggtgacgggc gcgaaggcgg
     1921 ccgacaagac ctactaccgt cacagcggcc atccgggcgg cgtctacgag acgagcttcg
     1981 agaagatgca gaaccgcttc ccgggccgcg tgctgcagat cgcggtgaag ggcatgctgc
     2041 cgaagggacc gctcggctac gcgatgatca agaagctcaa aatctacgcc gacggcgcgc
     2101 atccgcacac ggcgcagcaa cccaaggcgc tggagatctg aggcaaagat gattggcagc
     2161 tacaactacg gcaccggccg ccgcaagagt tcggtcgccc gcgtgttcat caagcgcggc
     2221 accggcaaga tcaccgt
//
LOCUS       DBNHBY010000031         7757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_68769, whole genome shotgun sequence.
ACCESSION   DBNHBY010000031 DBNHBY010000000
VERSION     DBNHBY010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_68769"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /gene="paaN"
                     /locus_tag="AC16HB_00780"
     CDS             complement(<1..1518)
                     /gene="paaN"
                     /locus_tag="AC16HB_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HIR5605352.1"
                     /translation="MTHPLLDKHRATLDGALNAIATRGYWSPYPEMPSPKVYGEGAQE
                     SGKAAVQALAGGRFALDQPGQTGWVASDRSPYGVKLDIQYPVCDPEALLAAAERAMPA
                     WGRLGVDGRTGVCLEILDRLNKASFEVAHAVMLTTGQGWMMAFQAGGPHAQDRGLEAV
                     AYAYREMSFVPAVARWEKPQGKNPPLAMEKHFEVVGRGVALVIGCSTFPTWNTYPGLF
                     AALATGNPVIVKPHPWSVLPAAISVRIARAVLAENGLDPNLVTLAAVDKPETTQWLAT
                     HAKVKSIDFTGGATFGRWLLTQTRAHAHDKQVYAELSGVNNVVIESTDNYKGMLRNLA
                     FTLSLYSGQMCTTTKAILVPAGGIDTDAGRKSFDEVAADLGNAIDKFLSDPATACAVL
                     GAIQSPDTLKRIAEAPQVATAVLASRQLSHPEFPHAEVHTPVVLKCDAAQESVYMQER
                     FGPISIVVAVPGDASAVATLAERIVREHGVLTVGVYSKNPAFVDVMTGATQRAGVA"
     gene            1660..2454
                     /gene="paaG"
                     /locus_tag="AC16HB_00785"
     CDS             1660..2454
                     /gene="paaG"
                     /locus_tag="AC16HB_00785"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HIR5605353.1"
                     /translation="MAQYENIRLEVEAGVATLTLNRPDRLNSFTVAMHGEVRAALDAV
                     RADKAVRCLVLTGAGRGFCAGQDLSDRAVAPGAAPLDLGASIENHYKPLVLALRNLPL
                     PVIAAVNGVAAGAGANIALACDLVFAARSASFIQSFARIGLVPDSGGTWVLPRLVGNA
                     RALGLALLGDKLTAEQAEQWGLIWKCVDDDQLRPTVRKLAEQLAAGPTRGFGRTKQAI
                     YAATGLTFEASLDLERDFQRELGRSRDYREGVAAFMEKRAPNFTGE"
     gene            2456..3994
                     /gene="paaH"
                     /locus_tag="AC16HB_00790"
     CDS             2456..3994
                     /gene="paaH"
                     /locus_tag="AC16HB_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HIR5605354.1"
                     /translation="MTEPLPPSATIGVIGAGAMGAGIAQVAAAAGHRVLIYDSNPKAV
                     ESARSAMQRELAALAAKGKLAAAAADAIVARVQAAATLADLRPCALIVEAVVEDLKIK
                     QDLFAELEKIAGPDCLLATNTSSLSITAIGAALAHPERLAGMHFFNPPARMRLVEVVS
                     GMATTDAVAQAVAATARAWGKHTAFAKSTPGFIVNRLARPYYAEAWRVLAESAADHAT
                     LDAIMRDCGGFPMGPFELMDLIGHDVNLSVTKSVFDATFGDRRYMPSLAQQELVRAGW
                     LGRKSGRGIYHYGSGAVKPEPKVEEGADPVSRVTLVGSLGIATPIVTRLERAGVEVVR
                     VEKRSGAPGWLEIGSARVMLSDGCCASQRALDIGQPQLVLFDLALDYSTTKRLALARA
                     DQCGEAALRTVFATLAQAGFACSRLDDVAGMIVLRTIAMLVNEAADAVAYGICTMDAI
                     DESMKHGVNYPRGPMAWAEAVGLDFFARVLGNLRAHYGEERYRTSVLIQRRGWSGTGF
                     LDRP"
     gene            3991..4458
                     /gene="paaI"
                     /locus_tag="AC16HB_00795"
     CDS             3991..4458
                     /gene="paaI"
                     /locus_tag="AC16HB_00795"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HIR5605355.1"
                     /translation="MSESHGHGHRPDPQTVAEAVKRKMFENDFASKSLGMQIESIAPG
                     YARVTMTVRRDMLNGFGLCHGGIITTLADTAFAFACNSHNELTLASGIFIEILNPGHE
                     GDRLLAEAREVTTNGRLGLYDILVTNQHGKHVAVMRGRSHTMKGKHVVDFTAS"
     gene            4476..5789
                     /gene="paaK"
                     /locus_tag="AC16HB_00800"
     CDS             4476..5789
                     /gene="paaK"
                     /locus_tag="AC16HB_00800"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HIR5605356.1"
                     /translation="MPVKKPAPGDLEPIERASRDEVQALQLKRLQWTLKHTYDNVPHF
                     RRKFDAAGVQPSDLKTLADLAKFPFTTKADLRDNYPYGLFAVPREQVVRIHASSGTTG
                     KPTVVGYTRNDVDMWATVMARSIRAAGGRPGDIVHVAYGYGLFTGGLGAHYGAEKLGC
                     TVIPMSGGMTERQVQLIADFKPDIIMVTPSYMLAIAEEMERQGFDPRGSSLKVGIFGA
                     EPWTPAMRQAIESKVNMEAIDIYGLSEIIGPGVAQECVETKDGLTVWEDHFYPEIIDP
                     DSGAVLPDGEKGELVFTSLTKEALPMIRYRTRDLTRLLPPTARSMRRIEKITGRSDDM
                     LIIRGVNVFPTQVEEQILKQPELTPHYTLEITRPANLDEMTVHVEMGQELAYASQEAR
                     HAAARKLEHNIKNYIGVTTTVRLALPGGIERSIGKAKRILDKRPK"
     gene            5800..6807
                     /gene="paaA"
                     /locus_tag="AC16HB_00805"
     CDS             5800..6807
                     /gene="paaA"
                     /locus_tag="AC16HB_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR5605357.1"
                     /translation="MYTQAIDIPQGKEDKPRGPRSLTEVEYEAEFDVKVGRGDFIEPK
                     DWMPEAYRRTLVRQISQHAHSEVVGMLPEGNWITRAPTLKRKAILLAKVQDEGGHGLY
                     LYAAAETLGVSRDDLVEALHSGKAKYSSIFNYPTLTWADIGVIGWLVDGAAIMNQIPL
                     TRCSYGPYARAMIRICKEESFHQRQGFDLLLTMMQGTAEQRALVQDATNRWWWPSLMM
                     FGPHDKDSPHSGDSMKWGIKRISNDDLRQKFVDATVPQARVLGVTLPDPLLAWNEERG
                     HWDFGPIDWSEFWNVVAGHGPCAKDRLAARVQAWEDGAWVREAAVAYAEKQALRERQA
                     A"
     gene            6944..7237
                     /gene="paaB"
                     /locus_tag="AC16HB_00810"
     CDS             6944..7237
                     /gene="paaB"
                     /locus_tag="AC16HB_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HIR5605358.1"
                     /translation="MSADRREWPLWEVFIRSRNGLDHKHCGSVHAPDARMALTNARDV
                     YTRRQEGVSLWVVRADHIHASDPDAKPEFFDPADDKVYRHPTFYRLPDEVNHM"
     gene            7314..>7757
                     /gene="paaC"
                     /locus_tag="AC16HB_00815"
     CDS             7314..>7757
                     /gene="paaC"
                     /locus_tag="AC16HB_00815"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR5605359.1"
                     /translation="MTTTVRANAAYLDYLLRIGDSSLILGQRLSEWCGHGPILEEDIA
                     LANIALDLIGQARLLLTHAGQVEGLERDEDALAFRRPEHAYRNVSMVELPNEDFGRTV
                     LRNLLYTSFQAQLWQGLAASADEQLAAIAAKSAKETRYHRQHSADW"
BASE COUNT         1328 a         2633 c         2661 g         1135 t
ORIGIN      
        1 cgccacgccg gcgcgctgcg tggccccggt catcacatcg acgaacgccg gatttttcga
       61 gtacacgccg acggtcagca caccgtgctc gcgcacgatg cgctccgcga gcgtggcgac
      121 cgccgacgca tcgcccggca ccgcgaccac gatcgagatc gggccgaagc gctcctgcat
      181 gtagaccgat tcctgcgcag cgtcgcactt gagcacgacc ggcgtgtgta cctcggcgtg
      241 cggaaactca ggatgagaaa gctgcctcga cgccagcacg gccgtggcga cctgcggcgc
      301 ctcggcgatg cgcttcagcg tgtcgggcga ctggatcgcg cccagcaccg cgcacgcggt
      361 ggccgggtcg gaaaggaact tgtcgatcgc atttcccagg tccgcggcga cctcgtcgaa
      421 cgacttgcgg cccgcgtcgg tgtcgatgcc gccggcgggc acgaggatcg ccttggtcgt
      481 cgtgcacatc tggcccgagt acagcgacag cgtgaatgcc aggttgcgca gcatgccctt
      541 gtagttgtcg gtcgactcga tcaccacatt gttcacaccc gacaactcgg catacacctg
      601 cttgtcatgc gcgtgggcgc gcgtttgagt caacaaccag cggccgaagg tcgcaccgcc
      661 cgtgaagtcg atcgacttca ccttcgcgtg cgtggcgagc cactgcgtgg tctcgggctt
      721 gtcgaccgcg gccagcgtga cgagattcgg atcgaggccg ttctcggcca gcaccgcgcg
      781 cgcgatgcgc accgaaattg ccgccggcag tacgctccac gggtgcggct tgacgatcac
      841 gggattgccg gtggccagcg ccgcgaacag gcccggatag gtgttccacg tcggaaacgt
      901 gctgcagccg atcaccagcg ccacgccgcg gcccacgacc tcgaaatgct tctccatcgc
      961 gagcggcgga ttcttgccct gcggcttctc ccagcgcgcc accgcgggga cgaagctcat
     1021 ctcgcgatac gcgtacgcga ccgcctccag gccgcgatcc tgcgcgtgcg gcccaccggc
     1081 ctgaaacgcc atcatccagc cctggccggt cgtcagcatc accgcgtgcg cgacttcgaa
     1141 cgaagccttg ttcagccggt cgaggatctc gagacagacg cccgtgcggc catcgacgcc
     1201 gagccggccc cacgccggca tcgcgcgctc ggcggccgcg agcagtgcct ccgggtcgca
     1261 caccggatac tggatgtcga gcttcacccc gtacggcgag cgatccgacg cgacccagcc
     1321 ggtctgtccc ggctgatcga gcgcgaagcg gccgccggcc agcgcctgca ccgccgcctt
     1381 gccggactcc tgcgcgcctt cgccgtagac cttcgggctc ggcatctccg ggtacggact
     1441 ccagtaccca cgcgtggcga ttgcgttcag cgcgccgtcg agcgtcgcgc ggtgcttgtc
     1501 gagcagcgga tgggtcatgg tgtcgtcccg ggcttgccac aaaagggagg agaaactact
     1561 attgattgac cggtcggtca acgaatgaca atcccgcatc gaccgcgtat cgataccgcc
     1621 ccgctgcaat ccggccgcgg cggttcaacg aggagacgca tggctcagta cgagaacatc
     1681 cggcttgagg tcgaggccgg agtggccacc ctgacgctca accggcccga ccggttgaac
     1741 agcttcactg tcgcgatgca cggcgaggtg cgcgcggcgc tcgacgccgt gcgcgccgac
     1801 aaggcggtgc gctgcctcgt gctcaccggc gccggccgcg gtttctgcgc cgggcaggat
     1861 ctgtccgatc gcgcggtcgc accgggcgct gcgccgctcg acctcggcgc gtcgatcgag
     1921 aaccattaca agccgctggt gctcgcgctg cgcaacctgc cgctgccggt gatcgccgcg
     1981 gtcaacggcg tggcggccgg tgccggcgcc aacatcgcgc tcgcatgcga cctcgtgttc
     2041 gccgcgcgct cggcttcatt tatccagtcg ttcgccagga tcggactggt tccggactcc
     2101 ggtggtacgt gggtgctgcc gcgcctggtt ggcaacgcgc gcgcgctggg gctggcgctg
     2161 ctcggcgaca agctgaccgc ggagcaggcg gagcagtggg ggctgatctg gaaatgtgtc
     2221 gacgacgatc agctgcggcc gaccgtgcgc aagctggcgg agcagttggc ggccgggccg
     2281 acgcgcggct tcgggcgcac caagcaggcg atttacgccg cgaccggact gacgttcgaa
     2341 gcgagcctcg atctcgaacg agacttccag cgcgaactcg gccgctcgcg cgactatcgc
     2401 gaaggcgtgg cggcgttcat ggaaaagcgc gcaccgaatt tcaccggaga atgaagtgac
     2461 cgaaccgctg ccgccgtcgg caaccatcgg cgtgatcggc gccggcgcaa tgggcgccgg
     2521 catcgcgcag gtcgcggcag ccgccggcca ccgcgtgctg atctacgaca gcaatccgaa
     2581 ggcggtcgag tccgcgcgca gtgcgatgca gcgcgaactc gccgcgctcg cggccaaggg
     2641 caagctcgct gccgccgctg ccgacgcgat cgtcgcgcgc gtgcaggccg ccgccacgct
     2701 cgccgacctg cggccgtgcg cgctgatcgt ggaggcggtg gtcgaggacc tgaagatcaa
     2761 gcaggacctg tttgcggaac tggaaaagat tgccggcccg gactgcctgc tcgccaccaa
     2821 cacgtcgtcg ctgtcgatca ccgcgatcgg cgccgcactg gcgcatccgg aacggctggc
     2881 cggcatgcac ttcttcaacc cgccggcgcg gatgcgtctg gtcgaggtcg tcagcggcat
     2941 ggcgacgacg gatgcggtcg cgcaggcggt cgccgccacc gcgcgcgcat ggggcaagca
     3001 cacggcgttc gcgaaatcga cgccgggctt catcgtcaat cggctcgcgc ggccgtacta
     3061 cgcggaggcg tggcgcgtgc tcgcagaaag cgcggccgat cacgcgacgc tcgacgcgat
     3121 catgcgcgac tgcggcggct ttccgatggg tccgttcgag ctgatggatc tgatcggcca
     3181 cgacgtgaac ctcagcgtca ccaagtcggt gttcgacgcc accttcggcg atcggcgcta
     3241 catgccgtcg ctggcgcagc aggagctcgt gcgcgccggc tggctcggcc gcaagagcgg
     3301 acgcggcatc taccactacg ggtccggcgc ggtgaagccc gagccgaagg tcgaggaggg
     3361 cgccgacccg gtgtcgcgcg tgacgctggt cggctcgctc ggcatcgcca cgccgatcgt
     3421 cacgcgcctg gagcgcgccg gcgtcgaggt cgtgcgggtc gagaaacgca gcggcgcgcc
     3481 ggggtggctc gagatcggct cggcgcgcgt gatgctgtcc gacggttgct gcgccagcca
     3541 gcgcgcgctt gacatcgggc agccgcagct cgtgctgttc gacctcgcgc tcgactattc
     3601 gacgacgaag cggctggcgc tggcgcgcgc cgaccagtgc ggcgaagcgg ccctgcgcac
     3661 cgtgttcgcc acgctggcgc aggcgggctt cgcgtgctcg cggctcgacg atgtcgccgg
     3721 gatgatcgtg ctgcgcacga tcgcgatgct ggtcaacgaa gcggccgacg cggttgccta
     3781 tggcatctgc acgatggacg cgatcgacga gtcgatgaag cacggcgtca actatccgcg
     3841 cggcccgatg gcgtgggccg aggcggtcgg cctcgacttc ttcgcgcgcg ttctcggcaa
     3901 cctgcgcgct cactacggcg aggagcgcta tcgcacctcg gtgctgatcc agcgccgcgg
     3961 ctggagcggc acggggttcc tggaccggcc atgagcgaat cgcacggaca tggccaccgg
     4021 cccgatccgc agacggtcgc cgaggcggtc aagcgcaaga tgttcgagaa cgacttcgcg
     4081 tcgaagagcc tcggcatgca gatcgagtcg atcgcgcccg gctacgcgcg cgtgacgatg
     4141 acggtccgcc gcgacatgct gaacggcttc ggcctgtgcc acggcggaat catcacgacg
     4201 ctcgccgaca ccgcgttcgc gttcgcgtgc aacagccaca acgagctgac gctggcatcg
     4261 gggatcttca tcgagatcct gaatccggga cacgagggcg accgcctgct ggcggaggcg
     4321 cgcgaggtga cgaccaacgg ccggctcggc ctgtacgaca tcctggtcac caaccagcac
     4381 ggcaagcacg tcgcggtgat gcgcggccgc tcgcacacca tgaagggcaa gcacgtcgtg
     4441 gacttcacgg cgtcctgagg aagaggagat cgaccatgcc tgtgaagaag cccgcgcccg
     4501 gcgatctgga gccgatcgag cgcgccagcc gcgacgaggt gcaggcgctg cagctcaagc
     4561 gcctgcaatg gacgctgaag cacacctacg acaacgtgcc gcacttccgg cgcaagttcg
     4621 acgccgcggg cgtgcagccg tcggacctga agacgctcgc ggacctggcg aagttcccgt
     4681 tcaccaccaa ggccgacctg cgcgacaact atccgtacgg gctgttcgcc gtgccgcgcg
     4741 agcaggtcgt gcgcatccat gcctccagcg gcacgaccgg caagccgacc gtggtcggct
     4801 acacgcgcaa cgacgtcgac atgtgggcca ccgtgatggc acgctccatt cgcgctgccg
     4861 gcggccggcc cggcgacatc gtgcatgtcg cctacggcta tggcctcttc accggcgggc
     4921 tgggcgcgca ctacggcgcg gagaagctcg gctgcacggt gatcccgatg tcgggcggca
     4981 tgaccgagcg gcaggtccag ctgatcgccg acttcaaacc ggacatcatc atggtgacgc
     5041 cgtcgtacat gctggcgatc gccgaggaga tggagcgcca gggattcgac ccgcgcggct
     5101 cgtcgctgaa ggtgggcatc ttcggcgcgg agccgtggac gccggcaatg cggcaggcga
     5161 tcgagtccaa ggtgaacatg gaagcgatcg acatctacgg gctgtcggag atcatcggac
     5221 cgggggtggc gcaggaatgc gtggagacca aggacggcct gacggtctgg gaggaccact
     5281 tctaccccga gatcatcgat cccgactccg gcgcggtgct gcccgacggc gagaagggcg
     5341 aactggtgtt cacgtcgctc accaaggaag cgctgccgat gattcgctat cgcacgcgcg
     5401 acctcacgcg actgctgccg ccgaccgcgc gttcgatgcg gcggatcgag aagatcaccg
     5461 gccgctccga cgacatgctg atcattcgcg gcgtcaatgt gttcccgacc caggtcgagg
     5521 agcagatcct gaagcagccc gagctcaccc cgcactacac gctcgagatc acgcgcccgg
     5581 ccaatctcga cgagatgacg gtgcacgtcg agatggggca ggaactcgcc tacgcgtcgc
     5641 aggaagcgcg ccacgccgcc gcgcggaagc tggagcacaa catcaagaac tacatcggcg
     5701 tcaccacgac cgtgcggctg gcgctgcccg gcggcatcga gcgctcgatc ggcaaggcga
     5761 agcggatcct ggacaaacgg cccaaatagg agcagcgcga tgtacacaca ggccatcgac
     5821 attccgcagg gcaaggaaga caagccgcgc gggccgcgct cgctgaccga ggtcgaatac
     5881 gaagccgagt tcgacgtcaa ggtcgggcgc ggcgatttca tcgagccgaa ggactggatg
     5941 ccggaggcat accgcaggac gctggtgcgg cagatcagcc agcacgcgca cagcgaagtc
     6001 gtcggcatgc tgcccgaggg caactggatc acgcgcgcgc cgacgctcaa gcgcaaggcg
     6061 atcctgctcg ccaaggtgca ggacgagggc gggcacggcc tgtacctcta cgcggcggcc
     6121 gagacgctcg gcgtatcgcg cgacgacctc gtcgaggcac tgcattcggg caaggccaag
     6181 tattcgtcga tcttcaatta cccgaccctc acctgggccg acatcggcgt gatcggctgg
     6241 ctcgtcgacg gcgcagcgat catgaaccag atcccgctca cccgctgctc gtacggcccg
     6301 tatgcgcgcg cgatgattcg catctgcaag gaggaatcgt tccaccagcg ccagggcttc
     6361 gacctgctgc tgacgatgat gcagggaacg gcggagcagc gcgcgctggt gcaggacgcg
     6421 accaatcgct ggtggtggcc gagcctgatg atgttcggcc cgcacgacaa ggattcgccg
     6481 cactcgggcg actcgatgaa gtggggcatc aagcgcatca gcaacgacga cctgcggcag
     6541 aagttcgtcg acgcgaccgt gccgcaggcg agggtgctcg gcgtcacgct gcccgacccg
     6601 ctgctggcgt ggaacgagga acgcggccac tgggacttcg gcccgatcga ctggagtgag
     6661 ttctggaacg tcgttgccgg ccacggcccg tgcgcaaagg accgccttgc tgcgcgcgtg
     6721 caggcctggg aggacggcgc ctgggtgcgc gaagccgcgg tcgcctacgc ggagaagcag
     6781 gcgctgcgcg agcggcaggc ggcatgaacg caggcgcgag cgcgcgcgcg ggcgggggat
     6841 ccaagggggc cgccgctccc tttgcatcca cgcctcgccc tgcagccact cgcgtcgccg
     6901 aaaggctcgg gcgccgcacg aggaaatcct gaaaggaaga gagatgtccg cagaccgccg
     6961 cgaatggcca ttgtgggaag tcttcatccg cagccgcaac ggcctcgacc acaagcactg
     7021 cggcagcgtg cacgcgccgg acgcgaggat ggcgctcacg aacgcgcgcg acgtctacac
     7081 gcggcggcag gagggcgtga gcctctgggt cgtgcgggcg gaccacatcc acgcgtcgga
     7141 tcccgacgcc aagccggagt tcttcgatcc ggccgacgac aaggtctacc ggcacccgac
     7201 cttctacaga ctgccggacg aagtcaacca catgtgacgg cgatgtccga gagcgaagcg
     7261 atcggaaaca gcgccgtcgt ccccacgcag gcggggatcc aggagtcatt cgcatgacga
     7321 cgaccgtgcg tgcgaacgcc gcgtatctcg actatctgct gcgcattggc gactcgtcgc
     7381 tgatcctcgg ccagcgcctg tccgaatggt gcggccacgg gccgatcctc gaggaggaca
     7441 tcgcgctcgc caacatcgcg ctcgacctga tcggccaggc gcggctgctg ctcacgcacg
     7501 cggggcaggt cgagggtctc gagcgcgacg aggacgcgct cgcgtttcgc cgccccgagc
     7561 acgcgtaccg caacgtctca atggtcgagc tgccgaacga ggacttcggc cgcacggtgc
     7621 tgcgcaattt gctgtacacg tcgttccagg cgcagctgtg gcaggggctc gcggcatccg
     7681 ccgacgagca actcgccgcg atcgcggcca agagcgccaa ggaaacgcgc tatcaccgcc
     7741 agcattctgc cgactgg
//
LOCUS       DBNHBY010000032         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1287018, whole genome shotgun sequence.
ACCESSION   DBNHBY010000032 DBNHBY010000000
VERSION     DBNHBY010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1287018"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16HB_00820"
     CDS             <1..186
                     /locus_tag="AC16HB_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5605360.1"
                     /translation="AAPDHLPQPLVEQLKPGGRLVIPVGSQIGGQDLLVIEKSADGRA
                     IKRTALPVRFVPLTRER"
     gene            424..1452
                     /locus_tag="AC16HB_00825"
     CDS             424..1452
                     /locus_tag="AC16HB_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605361.1"
                     /translation="MSRQFAVNHQALRLAACIGAILAAGCGGGGGGEVVAPPPELLQI
                     TTQNQTAVARATALAFGTLDSIRDIPSSAVPPAVQTSAASGGVKHALGKALAAAQAAG
                     AVRPQAVSAQTVPCPAGGSISITVDDRDNNGVPSRGDVLTMTFNDCRETASSLVRGGI
                     GVNITNYAYPLMSGLFTFNQLSVADETGSAAVSGAADCDYTSSTDPSGAVTTRLAMTV
                     GAAGLVGSMSAPTMQETVTYDPGFGATFTDVQLPTQSGYGESVANGKAHFASLGGKIS
                     LRTDAAIHDSWAENYPSSGRIVVDGYRSHLRMTVMNTTTMRLELDDNDDGAIEASRDM
                     AWKDLLPF"
     gene            1705..>1930
                     /locus_tag="AC16HB_00830"
     CDS             1705..>1930
                     /locus_tag="AC16HB_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5605362.1"
                     /translation="MQARSLDALDAQLHDWVRKAERLVDHDCVNLYAGTNTMNPRAKA
                     LLASSIGVRPSLGHPGEKYNKGVHEIERIEV"
BASE COUNT          331 a          655 c          662 g          282 t
ORIGIN      
        1 gcggcgcccg atcacctccc gcagccgctc gtcgaacagc tgaagcccgg cgggcggctg
       61 gtgatcccgg tcggcagcca gatcggcggg caggacctgc tggtgatcga gaagagcgcc
      121 gacggccgcg cgatcaagcg caccgcactg ccggtgcgct tcgtgccgct gacgcgcgag
      181 cgatgaagcg tccagtcgcc ggtcgcagcg agctgcgtcc attcccgggc gcatgcctcg
      241 tgctggaaag cgcacgcgaa ctatcccggg tcaacacggg ttgccagccg atggctaggt
      301 tgcacttgtg cggtgggaac gtggcgtcgc cacggcggtt cgaatcatcc cacctgccgt
      361 ggccgactcg aacgccgcgc cggcctggat gccgatccgg gccgaatcag cggggagaag
      421 aacatgtcga gacagttcgc agtcaatcat caggcgctac ggctcgccgc ctgcatcggc
      481 gcgatccttg ctgccggttg cggcggcggt ggcggcggag aggttgtagc cccgcccccg
      541 gagctgctgc agatcaccac ccagaaccag actgccgttg cgcgcgcgac cgcgctcgct
      601 ttcggcacgc tggacagtat tcgcgatatt ccgtcgtcgg cggtgccgcc cgcagtgcag
      661 acgagcgccg catccggtgg tgtcaagcat gcgctcggca aagcccttgc ggctgcgcaa
      721 gcggctggcg ccgtgcggcc gcaggcagtc agcgcgcaga ccgtgccgtg tccggccggc
      781 ggctcgatca gcatcaccgt cgatgaccgc gacaacaacg gcgtgccgtc gcgaggcgac
      841 gtgctgacca tgacattcaa cgactgccgc gagaccgcgt cgtcgctggt caggggaggc
      901 atcggcgtca atatcacgaa ctacgcctac ccgttgatga gcgggctgtt cacattcaac
      961 cagctgtcgg tggccgatga gaccggatcg gccgcggtca gcggcgcggc agactgcgac
     1021 tacacgtcga gcaccgatcc gtcgggcgcc gtgaccacgc gactcgcgat gacggtcggc
     1081 gcggcgggtc tggtcggcag catgtcggcg ccgaccatgc aggaaaccgt cacctacgat
     1141 ccgggcttcg gcgcgacctt caccgacgtg cagctgccca cccaatccgg ctacggtgaa
     1201 tcggtcgcga acggcaaggc gcacttcgcc tcgctcgggg gcaagatcag cctcaggacc
     1261 gatgcggcga tacacgattc ctgggccgag aactatccaa gcagcggcag gatcgtcgtc
     1321 gacgggtatc ggtcgcatct gcgcatgacc gtgatgaaca ccacgacgat gcgcctcgaa
     1381 ctcgacgaca acgacgatgg ggcgatcgaa gcgagccgtg acatggcgtg gaaggacttg
     1441 ctgccgttct gaccggcagc cgaaggcgca cggcattcct ccgcgtgcag tcggccgacg
     1501 ccgcacgggg ccggcccgcg cggctcggcg ggctgtacgg acgacggccc gagcgctccg
     1561 gcggcaatcg tcgacacctg ggcgctttcc agccggccac gcaatagact gtttccggtg
     1621 aacaccgcca atacccctcg gatgcccgcg cggccgggcg caagcgatgg tgcgaatcgg
     1681 ttccattccg atctcgcggc gcagttgcag gcgcggtcgc tcgacgcgct ggatgcgcag
     1741 ttgcacgact gggtccggaa ggccgagcgc ctggtcgacc acgactgcgt caacctgtac
     1801 gccggcacca acacgatgaa cccgcgcgcc aaggcgctgc tggcgtcgtc gatcggcgtg
     1861 cggccgagcc tcggccaccc cggcgagaag tacaacaagg gcgtgcacga gatcgagcgc
     1921 atcgaggtgc
//
LOCUS       DBNHBY010000033         1508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1557752, whole genome shotgun sequence.
ACCESSION   DBNHBY010000033 DBNHBY010000000
VERSION     DBNHBY010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1557752"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="AC16HB_00835"
     CDS             <1..1115
                     /locus_tag="AC16HB_00835"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5605363.1"
                     /translation="FPREDAFDIVVASEVMAIFCLATSLADLKERLGRIVVGYRRDLA
                     PIRAQDLKAHGAMAALLKDALKPNLVQTLENNPAIIHGGPFGNIAHGCNSVIATRAAL
                     KLGEYVVTEAGFGADLGAEKFIDIKCRKSGLRPDAAVIVATIRALKYHGGVPVDALTQ
                     ENLPALERGIVNLERHLNNVRNHFGLPCAIAVNHFDADTDAEIALLQRKMAHHGAAVI
                     VTRHWAEGGKGAEDLAREVVRLAAGGNADCRFLYEDDAPLWQKMKTVATRIYGASDIT
                     ADTKVRAQIRKLQDEGYGKLPVCVAKTQYSFSTDPLLRGAPSNHVVNIREVRLAAGAE
                     FIVMICGDIMTMPGLPKDPAAARIDIDDKGRIVGLF"
     gene            1120..>1508
                     /locus_tag="AC16HB_00840"
     CDS             1120..>1508
                     /locus_tag="AC16HB_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5605364.1"
                     /translation="MPLLAVDNLRVTFATDHGPARAVDDVSFSIEPGETVCLVGESGC
                     GKSVTALAIMGLLARPPAAVEGHIEFEGRDLLRLPNRMLADLRGDRLAMIFQEPMTSL
                     NPAFTIGAQLCEVLVRHRGAGAREARDA"
BASE COUNT          264 a          495 c          526 g          223 t
ORIGIN      
        1 ggtttccgcg cgaggatgcc ttcgacatcg tggtggcaag cgaggtgatg gcgatcttct
       61 gcctcgccac gtcgctggcg gacctgaagg agcggctcgg ccggatcgtg gtcggttacc
      121 ggcgcgacct ggcgccgatc cgcgcgcagg acctgaaggc gcacggcgcg atggccgcgc
      181 tgctgaagga cgcgctgaag cccaatctcg tgcagacgct ggagaacaac cccgcgatca
      241 tccacggcgg accgttcggc aacattgcgc acggctgcaa ctcggtgatc gccacacgag
      301 cggcgctgaa gctcggcgaa tacgtcgtga cggaagccgg cttcggtgcg gacctcggcg
      361 cggagaagtt catcgacatc aagtgccgca agtcgggact gcgtccggac gcggcggtca
      421 tcgtcgcgac gatccgggcg ctgaagtacc acggcggcgt gccggtcgac gcgttgacgc
      481 aggagaatct gccggcgctc gagcgcggca tcgtcaacct ggaacggcac ctgaacaacg
      541 tgcgcaacca tttcggcctg ccgtgcgcga tcgcggtcaa ccatttcgac gccgacaccg
      601 acgccgagat cgccctgctg cagcggaaaa tggcgcacca cggtgcggcg gtgatcgtga
      661 cccgccactg ggccgagggc ggcaagggcg ccgaagacct cgcgcgcgaa gtcgtgcggc
      721 tggccgcagg cggcaacgcc gactgccgct tcctgtacga ggacgacgcg ccgctgtggc
      781 agaagatgaa gacggtggcg acgaggatct acggcgcttc cgacatcacg gccgacacca
      841 aggtacgcgc gcagatccgc aagctgcagg acgaaggcta cggcaagctg ccggtgtgcg
      901 tggccaagac gcagtattcg ttctcgaccg atcccctgct gcgcggcgcg ccgtcgaacc
      961 acgtggtcaa catacgcgag gtgcggctcg ccgccggcgc cgaattcatc gtcatgatct
     1021 gcggcgacat catgacgatg ccgggcctgc cgaaggaccc ggccgccgca cggatcgaca
     1081 tcgacgacaa gggacggatc gtcgggctat tctgaatcga tgccgttgct cgccgtcgac
     1141 aatctgcgcg tcacgttcgc aaccgaccac ggcccggcgc gcgcggtcga cgacgtgtcg
     1201 ttttccatcg agcccggcga gaccgtgtgc ctggtcggcg agtccggctg cggcaagagc
     1261 gtcaccgcgc tggcgatcat gggactgctg gcgcggccac cggcagcggt cgaaggccac
     1321 atcgaattcg aaggccggga cctgctgcgg cttcccaatc gcatgctcgc cgacctccga
     1381 ggcgaccggc tggcgatgat cttccaggag ccgatgacgt cgctgaatcc ggcgttcacg
     1441 atcggcgcgc agttgtgcga agtgctggtc cgtcaccggg gcgcaggcgc gcgcgaggcg
     1501 cgcgatgc
//
LOCUS       DBNHBY010000034         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_678564, whole genome shotgun sequence.
ACCESSION   DBNHBY010000034 DBNHBY010000000
VERSION     DBNHBY010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_678564"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="AC16HB_00845"
     CDS             <1..1736
                     /locus_tag="AC16HB_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR5605365.1"
                     /translation="KSKTVLHNNIFHCVGDAAGISVEVAMQWNDGYSESLLAFTNNIP
                     QKDGGTHITGLRAAMTRALNKYIAEHEFDKRAKVETTGDDMREGLACVLSVKVPEPKF
                     SSQTKDKLVSSEVRPAVEEVVGRALESFLLERPADAKIICTKIVEAARAREAARKARE
                     MTRRKGLLDGAGLPGKLADCQERDPAKCELYIVEGDSAGGSAKQGRDRKFQAVLPLRG
                     KVLNVEKARFDKLISSEQIATLITALGTGIGPEFNPDKLRYHRIIIMTDADVDGAHIR
                     TLLLTLFYRQMPQLVERGHIYIAQPPLYKVKHGKDERYIKDDHELSLYLLQLALDGAR
                     LYPAAAAVDADQPIAGSALAELARQYLLADAVVQRLANVIDRGALDAIMNGVALDLRD
                     AAAAEASASTLEAAMGNHTVSVYAQYDSVTDKHRLRIDRRHHGNIKKSQIDAEFTHGA
                     DYAALAEAARTFKGLVGAGARVAREDRKNAGDKAKEAPVADFRAAMQWLLAQAESGVG
                     KQRYKGLGEMNPEQLWETTMDPAVRRLLKVQIEDAIGADQIFTTLMGDEVEPRRQFIE
                     INALAAGNIDV"
     gene            complement(1783..>2008)
                     /locus_tag="AC16HB_00850"
     CDS             complement(1783..>2008)
                     /locus_tag="AC16HB_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COG4315 family predicted lipoprotein"
                     /protein_id="HIR5605366.1"
                     /translation="NPALAGGDFSIVARDDGARQWAYKGMPLYFFAADQQPGDMKGDG
                     QGGVWFTARTKSGAANTAEQDYASSYSNKY"
BASE COUNT          379 a          695 c          665 g          269 t
ORIGIN      
        1 acaagtccaa gacggtcctg cacaacaaca tcttccactg cgtcggcgac gcggccggca
       61 tcagcgtcga agtcgcgatg cagtggaacg acggctacag cgaaagcctg ctcgcgttca
      121 ccaacaacat cccgcagaag gacggcggca cgcacatcac cggcctgcgc gcggcgatga
      181 cgcgcgcgct caacaagtac atcgccgagc acgagttcga caagcgcgcc aaggtcgaaa
      241 cgaccggcga cgacatgcgc gaaggcctcg cgtgtgtgct gtcggtcaag gtgccggagc
      301 cgaagttcag ctcgcagacc aaggacaagc tggtgtcgag cgaagtgcgc cccgcggtgg
      361 aggaagtggt cggccgcgcg ctggaaagct tcctgctcga gcgccccgcc gacgccaaga
      421 tcatctgcac caagatcgtc gaggcggcgc gcgcgcgcga agccgcccgc aaggcgcgcg
      481 agatgacccg ccgcaagggc ctgctcgacg gcgccggctt gcccggcaag ctcgccgact
      541 gccaggagcg cgacccggcg aagtgcgagc tctacatcgt cgagggtgat tcggccggtg
      601 gctccgccaa gcagggccgc gaccgcaagt tccaggcggt gctgccgctg cgcggcaagg
      661 tgctgaacgt cgagaaggcg cgcttcgaca agctgatctc gtcggagcag atcgccacgc
      721 tgatcaccgc gctcggcacc ggcattggcc ccgagttcaa cccggacaag ctgcgctacc
      781 accgcatcat catcatgacg gacgcggacg tggacggcgc gcacatccgc acgctgttgc
      841 tgacgctctt ctatcggcag atgccgcagc tcgtcgagcg cggccacatc tacatcgcgc
      901 aaccgccgct gtacaaggtc aagcacggca aggacgaacg ctatatcaag gacgaccacg
      961 aactgtcgct ctacctgctg cagctcgcgc tcgacggcgc gcggctgtat ccggccgcgg
     1021 ccgcggtcga cgccgaccag ccgatcgccg gcagcgcgct cgcggagctt gcgcgccagt
     1081 acctgctcgc cgacgcggtc gtgcagcgac tcgccaacgt gatcgaccgc ggcgcgctcg
     1141 acgcgatcat gaacggcgtc gcactcgatc tgcgcgacgc tgccgcggcc gaggcctcgg
     1201 caagcacgct cgaggcggcg atgggcaacc acacggtcag cgtctatgcg cagtacgaca
     1261 gcgtgaccga caagcaccgg ctgcgcatcg atcgccgcca ccacggcaac atcaagaaga
     1321 gccagatcga cgccgagttc acgcacggcg ccgactacgc ggcgctcgcc gaagccgcgc
     1381 gcaccttcaa ggggctggtc ggcgcgggcg cgcgcgtggc gcgcgaggat cgcaagaacg
     1441 cgggcgacaa ggccaaggaa gcgccggtcg cggactttcg cgccgccatg cagtggctcc
     1501 tcgcgcaggc cgaatccggc gtcggcaagc agcgctacaa gggcctgggc gagatgaatc
     1561 ccgagcagct gtgggaaacc acgatggacc ccgcggtgcg tcggctgctg aaggtgcaga
     1621 tcgaagacgc catcggcgcc gaccagatct tcaccacgct gatgggcgac gaggtcgagc
     1681 cgcgccgcca gttcatcgaa atcaacgcgc tcgcggccgg gaacatcgac gtttgaaccg
     1741 ggagacgact gcgcgtcgcc cccggcacgc acggcgcagc gtctaatact tgttcgagta
     1801 gctgctcgcg tagtcctgtt cggcagtgtt cgccgcgccg gacttcgtcc ttgcagtgaa
     1861 ccacacgccg ccttggccgt cgcccttcat gtcgccgggt tgctgatcgg ccgcgaagaa
     1921 gtacagcggc atgcccttgt aagcccactg ccgggcgccg tcgtcgcgcg cgacgatcga
     1981 gaagtcgccg ccggcaagcg ccggattg
//
LOCUS       DBNHBY010000035         6445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_475468, whole genome shotgun sequence.
ACCESSION   DBNHBY010000035 DBNHBY010000000
VERSION     DBNHBY010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_475468"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC16HB_00855"
     CDS             complement(<1..845)
                     /locus_tag="AC16HB_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR5605367.1"
                     /translation="MSTSLLAKLLLVALFVTSAAYVHLRGRVRFRFARQFFNHSTLLA
                     PYNALMYLFSAVPAKPVIDPADFPELAPLAADWRTIREEAQHLFDEGYIRTALAHNDV
                     GFNSFFKRGWKRFYLKWYGDPLPSAAALCPRTVALLQGIGSVKAAMFALLEPGAQLNP
                     HRDPFAGSLRYHLGLITPNSEACFIDVDGERLVWRDGVPLMFDETFIHWARNDTDVTR
                     VILFCDVERPLRTRFMRWLNRVISASLVKASATQNVPTEPVGAINWIYGNIVHPVTAR
                     VNAFFA"
     gene            1052..1249
                     /locus_tag="AC16HB_00860"
     CDS             1052..1249
                     /locus_tag="AC16HB_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR5605368.1"
                     /translation="MENKTARFTLLLDPRKKALFEAICAEQDLTPSQVVRQLMREYII
                     EHAGKRPLPAWLLAASGRKRR"
     gene            1260..3269
                     /gene="hyfB"
                     /locus_tag="AC16HB_00865"
     CDS             1260..3269
                     /gene="hyfB"
                     /locus_tag="AC16HB_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR5605369.1"
                     /translation="MTDWLPIDWLLVAVALWLAVGGAGLVRPTSFFIVARVLFPLGAA
                     VGLIVAGAALATLFTGAQVAVLPLGLPGLPFHLRLDALSAYFLLLLGSAAAGISVFAS
                     GYFRRGEGTPPGLLCLLYHVFLAAMALVIVADDAYVFMVMWELMALASFFLVTANHLI
                     PEIRRAGYLYLLIAHIGALAILLCFGVLQANTGDYTFDNMRAQHLSTFLASAGFLLAL
                     AGFGAKAGLLPLHVWLPEAHPAAPSPVSALMSGVMLKTAIYGLLRVSFDLLHVQLAWW
                     GVLALVLGLVTALFGVVFSAIQTDMKRLLAYSSIENIGLLAIGIGLALLFAGYGMKAL
                     AALALTAVLYHALNHAFFKSLLFLATGAVLHATGERNLGKLGGLLRTMPWVGWLALLG
                     ALAAAGLPPLNGFVSEWLLLQSFLFTLGLPGTFVNNLVPVTAAGVALVAALGGYVMVK
                     FFGVIFLGQPREPTLAHAHDAAAWERVGLVWLALACVLLGLAPAQVIALIDPVTRVLV
                     GEGVGTRVAASGWLFLAPVDPDRASYSPLLFLLLVAASYALAFLLVRWLYHGRVRSAA
                     PWDCGFPLQTARMQDTAEGFGQPIRQVFEPFFRIERTLPTPFDRAPVYRMVATDHFWQ
                     WLYLPVARVVEAASRLVGRLQQGRIATYLMYSFVTLLVMLVLVFR"
     gene            3266..4219
                     /locus_tag="AC16HB_00870"
     CDS             3266..4219
                     /locus_tag="AC16HB_00870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR5605370.1"
                     /translation="MSVYVYSALAQLLAILIAVAGAPLLTGWVNQCRAWLQNKSAPPL
                     LLPYRTLHKLFYKESVVAENASGLFRLAPYIVFGAMVLACGIVPSLSTDLPLAPAADA
                     IALVGLFALARVFMSLAAMDIGTAFGTLGARREMLVGFLAEPALLMVLFTASFLTQST
                     SLANIVETIGARELAIYPSLAFTGVAFTMVSLAENARVPVDNPATHLELTMIHEAMIL
                     EYSGRHLALIEWAASLKLFAYSSIGLAIFFPWGIAQVHDPLGMLLAAPALIAKLALGG
                     AALALIETVSAKMRIFRVPEFLAGAFLLAVIGMLVHFLLGA"
     gene            4223..4882
                     /locus_tag="AC16HB_00875"
     CDS             4223..4882
                     /locus_tag="AC16HB_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR5605371.1"
                     /translation="MSTLVTQLINLFAAILLLLSFALLSQRRVLSLIHLFTLQGATLV
                     LSTTVMAHFTQQPHLYWSALLTLLLKVLLMPWLLHRLINRLNVRWDVETLINIPTTML
                     LGIVLVIFAFNLAAPISSLSASVARGTLGIAMACVLLSFLMMITRTKAIPQVIGFLAM
                     ENALFFAATSATYGMPMVVELGIALDVLVGMVVLGVFMFQIREQFDSLDIRHLERLKE
                     E"
     gene            4891..6348
                     /locus_tag="AC16HB_00880"
     CDS             4891..6348
                     /locus_tag="AC16HB_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR5605372.1"
                     /translation="MEALLLVLGLPLVGAALLALVGHRDMAPELNVAASLATFVAAGV
                     LTAQVISGGPMLVLQQQFFVDSLNVFLVTLTAFVGLTTSLFARPYMRIERDHGKMTPP
                     RMRLFHSMYQLFTFTMLLALLTNNMGILWVALEAATLATVLLVAVYRTPASLEAAWKY
                     FILCGVGIAQALFGTILLYLAAERVIGAEGGGLLWTNLDAVKAQLEPTVIALAFVFLL
                     VGYGTKVGLAPLHNWLPDAHAEGPTPISAVLSGLLLNVALYAVLRCKVLTDGALGTRF
                     AGDLLMGFGLLSVVLAAFFLSRQRDIKRMFAYSSIEHMGLIAFAFGMGGPIASFAGLL
                     HMTVHSLVKSAIFFTVGHAAQKAGTQIMDNIRGLIRVNPTVGWGLMIGTLAILGMPPF
                     GVFASEFLILTTAMREFPWTTPLLLLALGVAFAAVLGKVQPMVFGETSFKPLAHPPAL
                     IPVFVHLGLALLLGLYIPAYLNGWYQQAARLIAGS"
BASE COUNT          851 a         2192 c         2191 g         1211 t
ORIGIN      
        1 gcgaagaatg cgttcacgcg cgccgtgacg ggatgcacga tgttgccgta gatccagttg
       61 atcgccccga ccggctcggt cggcacgttc tgcgtggcgc tcgccttcac gaggctggcg
      121 ctgatcacgc gattgagcca gcgcatgaag cgcgtgcgca gcggccgctc gacgtcgcag
      181 aacaggatca cgcgcgtgac gtcggtgtcg ttgcgcgccc agtggatgaa ggtctcgtcg
      241 aacatcagcg gcacgccgtc gcgccacacc agccgctcgc cgtcgacgtc gatgaagcac
      301 gcctccgagt tcggcgtgat cagcccgagg tggtagcgca gcgagccggc gaacggatcg
      361 cggtgggggt tgagctgcgc gccgggctcc agcagcgcga acatcgccgc cttgaccgag
      421 ccgatcccct gcagcagcgc gaccgtccgc gggcatagcg ccgccgccga cggcagcgga
      481 tccccgtacc acttcagata gaagcgcttc cagccgcgct tgaagaacga gttgaagccg
      541 acatcgttgt gcgcgagcgc ggtgcggatg tagccctcgt cgaacaggtg ctgcgcttcc
      601 tcgcggatcg tgcgccagtc ggccgccagc ggcgcgagtt ccggaaagtc cgccggatcg
      661 atgaccggtt tcgccggcac cgccgagaac aggtacatca gcgcgttgta cggcgccagc
      721 agcgtcgagt ggttgaagaa ctggcgcgcg aaacggaagc gcacccgccc gcgcaggtgc
      781 acgtaggccg ccgaggtcac gaacagtgcg accagcagca gcttggcgag caacgacgtc
      841 gacacgcgaa tcagccagga actcgatcag cttcgattat cgcgcgactc gagcgccggg
      901 ccgcgccgtc gccgcgaaag cgggaacgcg gcgacatcgg cgggcggacg cgcgcattgc
      961 cgcccgcgcc gcgacgacca cgccgccgcg catttgcgtt tgatgcacat ctgattatca
     1021 tgtgggccgg acgcattccc cgtccgccgc cgtggaaaac aagaccgccc gcttcacgct
     1081 gctgctcgac ccgcgcaaga aggcgctgtt cgaggcgatc tgcgcggagc aggacctgac
     1141 gccatcgcag gtcgtgcgcc agctgatgcg cgagtacatc atcgagcatg cgggcaagcg
     1201 cccgctgccc gcctggctcc tggcggcgtc cggcaggaag cgacgctgag gcggccgcgg
     1261 tgaccgactg gcttccgatc gactggctgc tcgtcgccgt cgcgctgtgg ctcgcggtcg
     1321 gcggcgccgg cctcgtgcgc ccgacgagct tcttcatcgt cgcgcgcgtg ctgtttccgc
     1381 tcggcgccgc ggtcgggctc atcgtcgccg gcgccgcgct cgccacgctc ttcaccggcg
     1441 cgcaggtcgc ggtgctgccg ctcggactgc cggggctgcc cttccatttg cggctcgatg
     1501 cgctgtcggc gtacttcctg ctgctgctcg gctctgccgc ggccggcatc tcggtgttcg
     1561 cctcgggcta cttccgccgc ggtgaaggca cgccgccggg gctgctgtgc cttctgtacc
     1621 acgtgttcct ggcggcgatg gcgctcgtga tcgtcgccga cgacgcgtac gtgttcatgg
     1681 tgatgtggga gctgatggcg ctcgcgtcgt tcttcctcgt caccgccaat cacctgatcc
     1741 cggagatccg ccgcgccggc tacctgtatc tgctgatcgc gcacatcggc gcgctggcga
     1801 tcctgctgtg tttcggcgtg ctgcaggcga acaccggcga ctacacgttc gacaacatgc
     1861 gcgcgcagca tctgtcgacc ttcttggcgt cggccgggtt cctgctcgcg ctcgccggct
     1921 tcggcgccaa ggctggactg ctgccgctgc acgtctggct gcccgaagcg cacccggccg
     1981 cgccgtcgcc cgtgtcggcg ctgatgagcg gcgtgatgct gaagaccgcg atctacgggc
     2041 tgttgcgcgt cagcttcgac ctgctgcacg tgcaactcgc ctggtggggc gtgctggcgc
     2101 tggtgctggg gctggtcacg gccctcttcg gcgtcgtgtt cagcgcgatc cagaccgaca
     2161 tgaagcggct gctcgcgtat tcgtcgatcg agaacatcgg tctgctcgcg atcggcatcg
     2221 ggctcgcctt gctgtttgct ggctatggaa tgaaggcgct ggccgcgctc gcactgaccg
     2281 ccgtgctgta ccacgcgctc aatcacgcgt tcttcaagag cctgctgttc ctcgccaccg
     2341 gagcggtgct gcacgcgacc ggcgagcgca acctcggcaa gctcggcggg ctgctccgca
     2401 cgatgccgtg ggtcggctgg ctcgcattgc tcggcgcgct cgccgcggcc ggactgccgc
     2461 cgctgaacgg gttcgtttcg gaatggctgc tgctgcagag cttcctgttc acgctcgggc
     2521 tgcccgggac gttcgtgaac aacctggtac cggtcaccgc ggccggcgtg gcgctggtgg
     2581 ccgcgctcgg cggctatgtg atggtcaagt tcttcggtgt gatcttcctc ggccagccgc
     2641 gcgagccgac gctcgcgcac gcgcatgacg cggccgcatg ggagcgcgtc gggctcgtgt
     2701 ggctcgcgct cgcctgcgtg ctgctgggac tcgcgcccgc gcaagtgatc gcgctgatcg
     2761 atccggtcac gcgcgtgctg gtcggcgagg gcgtcggcac tcgcgtggcc gcgagcggat
     2821 ggctcttcct cgcgccggtc gatcccgacc gcgcgagcta cagcccgctg ctgttcctgc
     2881 tgctggtcgc ggcgagctac gcgctcgcgt tcctgctggt gcggtggctg taccacggcc
     2941 gcgtgcgcag cgcggcgccg tgggactgcg gcttcccgct gcagaccgcg cgcatgcagg
     3001 acacggccga agggttcggc cagccgatcc gccaggtgtt cgagccgttc ttccgcatcg
     3061 agcgcacgct gccgaccccg ttcgatcgcg cgccggtgta ccggatggtg gcgaccgacc
     3121 acttctggca gtggctgtat ctgccggtcg cacgcgtggt cgaagcggca tcgcgcctgg
     3181 tcggccggct gcagcagggc cggatcgcga cctacctgat gtacagcttc gtcacgctgc
     3241 tggtgatgct ggtgctggtg ttccgatgag cgtctacgtc tacagcgcgc tcgcgcaact
     3301 gctcgcgatc ctgatcgcgg tcgccggcgc gccgttgctc accggctggg tcaaccagtg
     3361 ccgcgcctgg ctgcagaaca agagcgcgcc gccgctgctg ctcccgtacc ggacgctgca
     3421 caagctcttc tacaaggaat cggtggtggc ggagaacgcg agcggactgt tccgtctcgc
     3481 gccgtacatc gtgttcggcg cgatggtgct cgcgtgcggc atcgtgccga gcctgtcgac
     3541 cgacctgccg ctggcgccgg ccgccgacgc catcgcgctg gtcggcctgt tcgcgctggc
     3601 gcgcgttttc atgtcgctgg cggcgatgga catcggcacc gcgttcggca ctctcggcgc
     3661 gcggcgcgaa atgctggtcg gctttctggc cgagccggcg ctgctgatgg tgctcttcac
     3721 cgcctcgttc ctcacgcagt cgacgtcgct cgcgaacatc gtcgagacga tcggcgcacg
     3781 cgaactggcg atctacccga gcctcgcgtt caccggcgtc gcgttcacga tggtgtcgct
     3841 ggccgagaac gcgcgtgtgc cggtcgacaa cccggccacc catctcgaac tgacgatgat
     3901 ccacgaggcg atgatcctcg agtactcggg gcgccacctc gcgctgatcg agtgggccgc
     3961 gagcctgaag ctgttcgcgt actcatcgat cggcctggcg atcttctttc catggggcat
     4021 cgcgcaggtg cacgacccgc tcggcatgct gctcgcggca cccgcgctga tcgccaagct
     4081 cgcgctcggc ggcgcggccc tggcgctgat cgaaaccgtg tcggccaaga tgcggatctt
     4141 ccgcgtgccc gagttcctcg ccggcgcctt cctgctcgcg gtgatcggca tgctggtgca
     4201 cttcctgctc ggggcttgac cggtgtcgac gctcgtcacc caactgatca acctgttcgc
     4261 cgcgatcctg ctgctgctgt cgttcgcgct gctgtcgcag cggcgcgtgc tgtcgctgat
     4321 ccacctgttc acgctgcagg gcgcgaccct ggtgctgtcg accacggtga tggcgcattt
     4381 cacgcagcag ccgcacctgt actggtcggc gctgctcacg ctactgctca aggtgctgct
     4441 catgccgtgg ctgctgcacc ggctgatcaa ccggctgaac gtgcgctggg acgtggagac
     4501 cttgatcaac atcccgacca cgatgctgct gggcatcgtg ctggtgatct tcgcgttcaa
     4561 cctggcggcg ccgatctcca gcctgtcggc gtcggtggcg cgcggcacgc tcggcatcgc
     4621 gatggcgtgc gtgctgctgt cgttcctgat gatgatcacg cgcaccaagg cgatcccgca
     4681 ggtgatcggc ttcctggcga tggagaacgc gctgttcttc gccgccacca gcgcgaccta
     4741 cggcatgccg atggtggtcg agctcggcat cgcgctcgac gtgctggtcg gcatggtcgt
     4801 gctgggcgtg ttcatgttcc agatccgcga gcagttcgac agcctcgaca tccggcatct
     4861 ggaacggttg aaggaggaat aggcgttccg atggaagcgt tgctcctcgt cctcggcctt
     4921 ccgctcgtcg gtgccgcgct gctcgcgctg gtcggccacc gcgacatggc gccggaactc
     4981 aatgtcgccg ccagccttgc caccttcgtc gccgccggag tgctgacggc gcaggtcatc
     5041 tccggcgggc cgatgctggt gctgcagcag cagttcttcg tcgattccct caacgtcttc
     5101 ctggtgacgc tgaccgcctt tgtcggcttg accacgtcgt tgttcgcgcg gccgtacatg
     5161 cgcatcgagc gcgaccacgg caagatgacc ccgccgcgca tgcgcctgtt ccacagcatg
     5221 taccagctgt tcacgttcac gatgctgctg gcgctgctga ccaacaacat ggggatcctg
     5281 tgggtcgcgc tggaagcggc cacgctcgcg accgtgctgc tggtcgcggt gtatcgcacg
     5341 ccggccagcc tcgaggcggc gtggaagtac ttcatcctgt gcggtgtggg catcgcgcag
     5401 gcgttgttcg ggacgatcct cctgtacctc gccgccgagc gcgtgatcgg ggccgagggc
     5461 ggcggcctgt tgtggaccaa tctggacgcg gtcaaggcgc agctcgagcc gacggtgatc
     5521 gcgctcgcgt tcgtcttcct gctggtcggc tacggcacca aggtggggct ggcgccgctg
     5581 cacaactggc tgcccgacgc acacgccgaa ggcccgacgc cgatttctgc ggtgctgtcg
     5641 gggctgctgc tgaacgtcgc gctgtacgcg gtcctgcgct gcaaagtgct gaccgacggt
     5701 gcgctcggca cgcgcttcgc cggcgacttg ctgatgggct tcggcctgct gtcggtggtg
     5761 ctggcggcgt tcttcctttc gcgccagcgc gacatcaagc gcatgttcgc gtattcgtcg
     5821 atcgagcaca tggggctgat cgcattcgca ttcggcatgg gcggcccgat cgcgagcttt
     5881 gccggcctgc tgcacatgac ggtgcattcg ttggtcaaat ccgcgatctt cttcacggtc
     5941 gggcatgcgg cgcagaaggc cggcacgcag atcatggaca acatccgcgg cctgatccgc
     6001 gtgaacccga ccgtcggctg ggggctgatg atcggcacgc tcgcgatcct cggcatgccg
     6061 ccgttcggcg tgttcgcgag cgaattcctg atcctgacca ccgcgatgcg cgagtttccc
     6121 tggaccacgc cgttgctgct gctcgcgctc ggcgtggcgt ttgccgccgt gctcggcaag
     6181 gtgcagccga tggtcttcgg cgagacgtcg ttcaagccgc tcgcgcaccc tcccgcgctg
     6241 attccggtgt tcgtccatct ggggctggcg ctgctgctcg gcctgtacat ccccgcgtac
     6301 ctgaacggct ggtaccagca ggccgcgcgg ctgatcgccg ggagctgaga tgcagatcgc
     6361 cggcgtcgat ttcgcagcgc tgcccggggc gatcgcggcg ggcgtcgcca cggtcgatga
     6421 ctcgcagtgg gtggatatcg cgaag
//
LOCUS       DBNHBY010000036         2435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1626053, whole genome shotgun sequence.
ACCESSION   DBNHBY010000036 DBNHBY010000000
VERSION     DBNHBY010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1626053"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..590)
                     /locus_tag="AC16HB_00885"
     CDS             complement(147..590)
                     /locus_tag="AC16HB_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR5605373.1"
                     /translation="MKVFNLHCDSGHAFEGWFGSADDFDAQFARGQLTCPLCGSQQVT
                     KALSAPYLATGASEKQLAAPQQAAMPTPAQMQALFVKFAREIVANTEDVGQRFAEEAR
                     RIHYKEAEERGIRGVTTKDEAAALEEEGINVVPLPFADLLKEPLQ"
     gene            830..914
                     /locus_tag="AC16HB_00890"
     tRNA            830..914
                     /locus_tag="AC16HB_00890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Leu,seq:taa)
     gene            1067..1354
                     /locus_tag="AC16HB_00895"
     CDS             1067..1354
                     /locus_tag="AC16HB_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605374.1"
                     /translation="MRDVSARFDICAEVELVKAKPLAFDQVRERGVSTERMRAPRSRG
                     AHSDREPVADDAQQRTLAVHRSGSTVDPLICREASIAAWSGSLDLMQVHAT"
     gene            1453..1671
                     /locus_tag="AC16HB_00900"
     CDS             1453..1671
                     /locus_tag="AC16HB_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605375.1"
                     /translation="MTITFDDGSNPEALGIVIPVAVHGEYLEAIVDPTTNLRKLFKLT
                     KIAAVRCAGGKLALNPAARASKVETALT"
     gene            complement(1739..1812)
                     /locus_tag="AC16HB_00905"
     tRNA            complement(1739..1812)
                     /locus_tag="AC16HB_00905"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1778..1780),aa:Cys,seq:gca)
     gene            complement(1890..1965)
                     /locus_tag="AC16HB_00910"
     tRNA            complement(1890..1965)
                     /locus_tag="AC16HB_00910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Gly,seq:gcc)
     gene            complement(2041..>2435)
                     /gene="pgsA"
                     /locus_tag="AC16HB_00915"
     CDS             complement(2041..>2435)
                     /gene="pgsA"
                     /locus_tag="AC16HB_00915"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR5605376.1"
                     /translation="MTSPLGAFLDPVADKLMVSAALVVLLSLDRVDSFVALVIIGREI
                     AVSALREWMAQLGKSSSVAVNSLGKIKTIAQMVAIPMLLFHDKLFGLIDVRMIGTVLI
                     YVAAVLTVYSMLVYLRMAWPHLNDEEDG"
BASE COUNT          437 a          776 c          773 g          449 t
ORIGIN      
        1 tggtcgaaaa tctggcgccg ctcggaatca agcgcaagga cgtgatggtg gtggtgcgcg
       61 acgtaccgcg cgagaactgg ggcatcatgg gcggcgtgcc cgcatcggac gtcgacctcg
      121 gattcaaggt cgatgtgtag ccgcgtctac tgcagcggct ccttcagcag atccgcgaac
      181 ggcagcggca cgacgttgat gccctcttcc tccaacgcgg ccgcctcatc cttggtcgtc
      241 acaccgcgga tgccgcgctc ctccgcctcc ttgtagtgga tgcgtcgcgc ttcctccgcg
      301 aagcgctggc cgacgtcctc ggtgttggcc acgatctccc gcgcaaactt cacgaatagg
      361 gcctgcatct gcgccggcgt cggcatcgcc gcctgctgcg gcgcggccaa ctgcttttcg
      421 gacgcgccgg tggcaaggta cggcgccgac agcgccttgg tcacttgctg cgagccgcac
      481 aacgggcacg tcagctggcc acgagcgaac tgcgcgtcga aatcgtcggc ggagccgaac
      541 cacccttcga acgcgtggcc gctgtcgcag tgcagattga agactttcag tgccattgaa
      601 gttgccggtg ccgcggccgg attcgaaccg gcttaccttc ttacggttgc agcgcattct
      661 aagtcacggt caatctctcc agcgtggcga acccggcaac agcgcattga tttcagaggg
      721 gatcatcact tgatctggcg caagcgcgcg ctcgtcggtt gcccattcgt gcgcgcttgc
      781 gatgcggagt gacgcgcgaa tgacgcacgg gataagatgc cgcgcctgtg cccgggtgac
      841 gaaactggta gacgtaccgg acttaaaatt cggggccgca aggcgtatgg gttcgactcc
      901 cgtcccgggc accacgccag cgcatcgatc tcggttccct tggccgtgaa tgggcagatg
      961 cgtgcatgct gtgatgtctt ggttgcgcaa tctgccgttg ccttgacctc atgtgctttg
     1021 tcgtcgcagc gccggaaggc gtcggcattg ccgctgactc ggtgacgtgc gcgacgtatc
     1081 ggcccgcttc gacatctgcg ctgaagtgga actggtgaag gctaagccgc tggcgtttga
     1141 tcaggtgcgg gaacgcggcg tgagcaccga gcggatgcgc gcgccacgta gccgcggcgc
     1201 gcattcggat cgcgagccgg tggcagatga cgcgcagcag cggacccttg cggttcatcg
     1261 gtcggggtcg accgttgatc ctttgatctg ccgtgaagca tcgattgccg cgtggtcagg
     1321 atctctcgat ttgatgcagg tccatgcaac ctgaactgcg ccgagtagcg tttcgcaaga
     1381 ttcgcagacg agcaggtcgt cggtatgtct gtaaccacgc tccttcgcgc agcggccctg
     1441 acgcacgatg ccgtgaccat cacatttgac gatggctcca atccggaggc gctgggtatc
     1501 gtcattcccg tcgcggtgca tggcgagtac ctggaagcga tcgtcgatcc gacgacgaac
     1561 ctccgcaagc tgttcaagct caccaagatt gcagcggtca ggtgtgccgg gggcaaactg
     1621 gcgctgaacc cggcggcgcg tgcttcgaag gtcgaaacgg cactgaccta gcctgccggc
     1681 gcccgctgct tcgaacctgc agacgtacgc cgcgcgatca tcgaattgcg gagagatttg
     1741 gaggcggggg tcggaatcga accggcgtac acggatttgc agtccgctgc atggccactc
     1801 tgccaccccg ccgccgcggc accgcgcagg gaaacgatgc gatgccgttc aaaccaaatg
     1861 gggaagcgcg gcggcttccc catgaaaact ggagcgggcg aagagtctcg aactctcgac
     1921 ctcaaccttg gcaaggttgc gctctaccaa ctgagctacg cccgcgctcg aagcggcgaa
     1981 ttatagctgc ggtcagaggg gtgtcaaacc aacgcgctct gctcgccgct cgttccgcac
     2041 tcagccgtct tcctcgtcgt tgaggtgcgg ccacgccatc cgcaggtaga ccagcatcga
     2101 gtacaccgtg agcaccgcgg ccacgtagat cagcaccgtc ccgatcatcc gcacgtcgat
     2161 cagcccgaac agcttgtcgt ggaagagcag catcgggatc gcgaccatct gcgcgatcgt
     2221 cttgatcttt ccgagcgaat tgaccgcgac gctgctcgac ttgccgagct gcgccatcca
     2281 ctcgcgcagg gccgagaccg cgatctcgcg gccgatgatc acgagcgcca cgaacgagtc
     2341 gacgcggtcg agcgacagca gcaccaccag cgcggcactg accatcagct tgtccgccac
     2401 cggatcgagg aacgcgccga gcgggctcgt catgc
//
LOCUS       DBNHBY010000037         6410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_137310, whole genome shotgun sequence.
ACCESSION   DBNHBY010000037 DBNHBY010000000
VERSION     DBNHBY010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_137310"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="AC16HB_00920"
     CDS             complement(<1..864)
                     /locus_tag="AC16HB_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR5605377.1"
                     /translation="MKTRKLKIAAAVAALFLPALAGATNGYLPHGYGMKALGMGGAGI
                     AYPQDAIAAALNPAGMAMLGNRIDLGLTWFRPTRESEIVGNAAGPAVNGTYNGNDESD
                     FFIPEFGYNRMLNQNLSLGVSVFGNGGMNTSYKRPVPLFGSSNPGVDLMQLFITPTVG
                     YKITPDHAVGLGVNLAYQRFKAHGLENFTAPSGPQQFSAFPGNVTNQGYDDSFGIGAR
                     VGYLGQLTPQFALGLTYQTKASMSKFDKYKGLFAEEGGFDIPATYGIGVAFKPTPAFN
                     VALDVVRIEYSG"
     gene            1176..1451
                     /locus_tag="AC16HB_00925"
     CDS             1176..1451
                     /locus_tag="AC16HB_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605378.1"
                     /translation="MLQRLAYWQRVFALRYSERIAVTPAAHTWQRLERTLDPPRYQSR
                     WYRRIGLWQGWPAAATAALELAVAVGPAGGSPTRAPTGPVILGEIRV"
     gene            1476..2501
                     /locus_tag="AC16HB_00930"
     CDS             1476..2501
                     /locus_tag="AC16HB_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HIR5605379.1"
                     /translation="MPRPHIEIAVVAALLAGAGAASAQPDPQALAACAAIDDPVARLA
                     CFDRLVPKPSGELRAPTAEPSLAVLAPVAEAPSLLGERWSIGVSGRDSRFDVRAHRAS
                     YFLPARYSNAPNRLPTSPSKDALTAPLDIEATEAKFQFSFKVKLADLDELIGASLWAG
                     YTQQSHWQVYNANVSRPFRETDYEPEAMLAFHPDRDVLGWHWRLLVLGLSHQSNGRSD
                     PLSRSWNRVYAQFGLEQGNFGVLIRPWWRVPESRAQDDNPDIQRYLGHGDIVGVYRWG
                     SHTLSALGRYNPGTRKGALQLNWNFPLARRVRGYLQAFTGYGESLIDYNVPQNTIGIG
                     ISLADHL"
     gene            2565..3551
                     /locus_tag="AC16HB_00935"
     CDS             2565..3551
                     /locus_tag="AC16HB_00935"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR5605380.1"
                     /translation="MKRLWLMALTALIAVAAPAWAQQQIVDVAHVKAAVARGALLWDA
                     RDEGTFAKGHIPGAVNIGAPTDELRDPNTEDYLPLPRLEKILGGAGLDPRREVIVYAQ
                     RGASSAYFAQFTLRYFGAQKVHVFHDGIDGWRAAGEKVATEPTAPTPVALKLVPKEGL
                     TIDTKAVIAAVRNPNVQIVDARTPKEFKGEDIRAIRGGHIPGAINIPYEQNWVDPDAI
                     GKMRRGELKDSTGMSLKPRDELRKLYAALDPAKETIVYCQSGVRAAETTAVLAELGFS
                     NVKLYDSSWLGYAAALSAPVDNEVFVNVGALNNQIRALEGRINALEVALQKR"
     gene            complement(3561..4043)
                     /locus_tag="AC16HB_00940"
     CDS             complement(3561..4043)
                     /locus_tag="AC16HB_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5605381.1"
                     /translation="MRFEHLVQINDPLNPLLEPLSRMQLWRGLVLRAEQPVQFVLGLA
                     GCAIRSREERAEQTTLARTLDFGSFSVQDHVMLTPQAHSQTRTDAGPTWPASRLTITI
                     EEPQPEQLFLRFVYEFEGADEGGDLAAEMQALRRQAYKYADLDTVQRIRALAERGELG
                     "
     gene            complement(4097..4825)
                     /gene="queC"
                     /locus_tag="AC16HB_00945"
     CDS             complement(4097..4825)
                     /gene="queC"
                     /locus_tag="AC16HB_00945"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR5605382.1"
                     /translation="MNPDQRALVLFSGGLDSTVCLAWALTRYAHVETVGFDYGQRHPV
                     ELDVRPQVLKALCDAMPEWRGRLGPDHLIKLAALSQIGHTALTSDARSRLADKSLPTT
                     FVPGRNLLFFTYAAAIGFRRNLHALVAGMSETDYSNYADSRDEVMKSLQRTLSLGMDQ
                     HFAIETPLMWLDKAQTLQLGDDLGGAKLIEVIRTETHSCFMGVRDKLHAWGRGCGICP
                     ACALRAAGWDHWQATRGAIVRSAS"
     gene            5011..5694
                     /locus_tag="AC16HB_00950"
     CDS             5011..5694
                     /locus_tag="AC16HB_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605383.1"
                     /translation="MDRTTGETLPIYLHQGRRYVAGTPGNRYAIAVRNRTGGRLLAVM
                     SVDGVNVVTGETAAWDQNGYVFDPGQRWEVRGWRKSQERIAAFEFTALPNSYAARTGR
                     PQHVGVIGVALFREAKAAPAPLEPQRREPGGTGAGETGAAVAAPEADRAERRRDASRL
                     GTGHGRSESSPVALTDFQRLRGTPDEVITVHYDSRDNLIALGVIPAVRAPVPNAFPGS
                     IGFVPDPPR"
     gene            5736..6338
                     /locus_tag="AC16HB_00955"
     CDS             5736..6338
                     /locus_tag="AC16HB_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5605384.1"
                     /translation="MRDAPAADVRSDEQLLLDYGAGDRDAFARLFDRHERPVYRFLLR
                     SLGRDAARADDLLQEVWLAVIRNASTWEPRARFTTWLYGIARSRLIDHWRAQKGEVSL
                     DAANDPEADDPGAALIDRIAGARNDEPEVRAMSRAQARAFVAAVERLPVAQREALLLH
                     LEGELTLDEIAQLTGVGMETAKSRLRYALAKLREWLEDWR"
BASE COUNT         1035 a         2154 c         2204 g         1017 t
ORIGIN      
        1 gccggagtac tcgatccgca ccacgtcgag cgccacgttg aaggcgggcg tgggcttgaa
       61 cgcaacgccg atgccgtagg tggccggaat gtcgaagcct ccttcctccg cgaacaggcc
      121 cttgtacttg tcgaacttgc tcatgctcgc cttggtctgg taagtcagac cgagggcgaa
      181 ctgcggcgtc agctggccca ggtaaccgac gcgcgcgcca atgccgaacg agtcgtcata
      241 gccctggttg gtcacgttgc ccggaaacgc ggaaaactgc tgcgggccac tgggcgcggt
      301 gaaattctcc aggccgtggg ccttgaagcg ctggtaggcg aggttcacgc cgaggccgac
      361 ggcgtgatcg ggcgtgatct tgtagcccac cgtcggcgtg atgaacagct gcatcaggtc
      421 gacgcccggg ttgctcgatc cgaacagcgg caccggccgc ttgtacgagg tgttcatgcc
      481 gccattgccg aacaccgaaa cgcccagcga aaggttctga ttcagcatgc ggttgtagcc
      541 gaactcgggg atgaagaagt cgctttcgtc gttgccgttg taggtgccgt tgaccgccgg
      601 ccccgcggcg ttgccgacga tctcggattc gcgcgtcggg cggaaccagg tcaggccgag
      661 atcgatccgg ttgcccagca tcgccatgcc ggctgggttg agggcggcgg caattgcatc
      721 ctgcggatag gcgatgccgg cgccgcccat gcccagcgcc ttcatgccat aaccgtgagg
      781 cagatagccg ttggtggcac ctgcgagcgc cggcaggaaa agcgccgcaa ccgcggccgc
      841 gatcttcagc tttctcgttt tcatcattcc tcccaagagg ggtttctgtt cggcagtacc
      901 aaacgtgagt aaccctaccc gcgtggcgcc tggcacggat gtcgggggtc aatcgcgtcg
      961 tttcacttcg acacgatcgg tgacttgacc cacactggcg tcgcttcgcg ccgacgaatc
     1021 ggtccgaatc cattttcagg taagggtcgt actgaaagga acactccttc gggaccaccc
     1081 cttcgggatt accccggcat gctcgacgag gtgatcatca agcgcctgct gctgcgcgcc
     1141 gccgcttcga gcgtgtgctg cgcgacgggc cccctgtgct gcagaggctc gcgtactggc
     1201 agcgcgtgtt cgcgctgcgc tacagcgaac gcatcgcagt gacgcccgcg gcgcacactt
     1261 ggcagcggct cgagcgcacg ctcgatccgc cgcgctacca gtcgcgttgg taccgccgca
     1321 tcgggctgtg gcagggctgg cccgcggccg ccaccgccgc gctcgagctc gcggtcgcgg
     1381 tcgggcccgc gggcggatcg ccgacgcgtg cgccgaccgg cccggtcatt ctcggcgaga
     1441 tcagggtctg agggcgcgct ggcacaatcg ccagcgtgcc gcgtccccac atcgagatcg
     1501 ccgtcgtcgc cgcgctgctg gccggcgcgg gcgcagcaag tgcgcagccc gacccgcaag
     1561 cgctggcggc gtgtgcggca atcgacgatc cggtggcgcg gctcgcctgc ttcgaccgcc
     1621 tcgtgccgaa gccgtccggc gaattgcgcg caccgacggc cgagccttcc ctggcggtac
     1681 tcgcacctgt cgcggaggcg ccgtcgctgc ttggcgagcg ctggtccatc ggcgtgtcgg
     1741 gacgcgacag ccgcttcgac gtgcgcgcgc atcgggccag ctacttcctg ccggcgcgct
     1801 acagcaatgc gccgaaccgg ctgccgacga gcccgagcaa ggatgcgctg accgcgccgc
     1861 tcgatatcga agccaccgag gcgaagtttc agttcagctt caaggtcaag ctcgccgatc
     1921 tcgacgaact catcggcgcg tcgctgtggg ccggctacac gcagcagagc cattggcagg
     1981 tctacaacgc caatgtctcg cgcccgttcc gcgagaccga ctacgagccg gaggcgatgc
     2041 tggcgttcca ccctgaccgc gacgtgctcg gctggcactg gcggctgctg gtgctggggc
     2101 tgtcgcacca atccaatggg cgcagcgatc cgctgtcgcg cagctggaac cgcgtgtatg
     2161 cgcagttcgg gctggagcag ggcaacttcg gcgtgctgat ccgcccgtgg tggcgagtgc
     2221 cggagagccg cgcgcaggac gacaaccccg atatccagcg ctatctcggc catggcgaca
     2281 tcgtcggcgt ctatcgctgg ggcagccaca cgctgtcggc gctcggccgt tacaacccgg
     2341 gcacgcgcaa gggcgcgctg cagctgaact ggaacttccc gctcgcgcgc cgcgtgcgcg
     2401 gctatctgca ggcgttcacc ggctatggcg agagcctgat cgactacaac gtgccgcaga
     2461 acacgatcgg catcggaatt tcgcttgccg atcatctgtg atccgcctca cggcggattg
     2521 ttcggatgct ggcatcctag ccacctaccg aaaggagaac tgggatgaag agactctggc
     2581 tgatggcact gacggccctg atcgcagtgg cagcacccgc ctgggcgcag cagcagatcg
     2641 tcgacgtcgc gcatgtgaag gcggcggtcg cgcgcggcgc gctgctgtgg gacgcacgcg
     2701 acgagggcac gttcgcaaag ggacacatcc ccggcgcagt gaacatcggt gcgccgaccg
     2761 acgagctgcg cgatccgaac accgaggact acctgccgtt gccgcggctc gagaagatcc
     2821 tgggcggcgc gggactggat ccgcggcgcg aggtgatcgt gtacgcgcag cgcggcgcgt
     2881 cgagcgcgta cttcgcgcag ttcacgctgc gctatttcgg cgcgcagaag gtccacgtgt
     2941 ttcacgacgg catcgacggc tggcgcgcgg cgggcgagaa ggtcgcgacc gagccgactg
     3001 ctccgacgcc ggtggcgctg aagctcgtgc ccaaggaggg actgacgatc gacaccaagg
     3061 cggtgatcgc tgcggtccgc aacccgaacg tgcagatcgt cgatgcgcgc acgcccaagg
     3121 agttcaaggg cgaggacatc cgcgccatcc gcggcggcca catccccggc gcgatcaaca
     3181 ttccgtacga acagaactgg gtcgatccgg acgcgatcgg caagatgcgc cgcggcgagc
     3241 tgaaggattc gacgggcatg tcgctcaagc cgcgcgacga actgcgcaag ctgtatgcgg
     3301 cgctcgatcc ggccaaggag acgatcgtct actgccagag cggcgtgcgc gcggcggaaa
     3361 cgacggcggt cctcgcggaa ctgggcttca gcaacgtgaa gctgtacgac tcgtcatggc
     3421 tcggctacgc ggcggcgctg tcggcgccgg tcgacaacga ggtgttcgtc aatgtcggcg
     3481 cgctgaacaa ccagatccgc gcgctcgaag gccgcatcaa cgcactcgag gtggcactgc
     3541 agaagcgtta ggcggagtcg tcagccgagc tcgccacgct ccgcgagcgc gcggatccgc
     3601 tgcacggtgt cgaggtcggc gtacttgtag gcctgccgcc gcagcgcctg catctcggcg
     3661 gccaggtcac cgccttcgtc cgcgccctcg aactcgtaga cgaaacgcag gaacaactgc
     3721 tcgggctgcg gttcctcgat cgtgatcgtc agccggctcg cgggccaagt cgggcccgcg
     3781 tcggtgcgcg tctgcgaatg cgcctgcggc gtcagcatta catggtcctg cacgctgaag
     3841 ctgccgaagt cgagcgtgcg cgccagcgtc gtctgttcgg cgcgctcctc gcgcgagcgg
     3901 atcgcgcatc cggcgagccc gagcacgaac tgaacgggct gctcggcgcg cagcaccagc
     3961 ccgcgccaca gctgcatgcg cgagagtggt tcgagcagtg gattgagcgg gtcgttgatc
     4021 tggaccagat gctcgaaacg catgcgtcga tcgcggcgtc ggccgaccgg ccacgccacc
     4081 cagtgtgcgc cacgcttcag gaggcggagc ggacgatcgc gccgcgcgtc gcctgccagt
     4141 gatcccagcc tgcggcgcgc agcgcgcatg cggggcagat gccgcagccg cggccccagg
     4201 cgtgcagctt gtcgcgcacg cccatgaagc agctgtgcgt ctcggtacgg atcacttcga
     4261 tcagcttcgc gccgccgagg tcgtccccca gttgcagcgt ctgcgccttg tcgagccaca
     4321 tcagtggcgt ttcgatcgcg aagtgctggt ccatgccgag cgacagcgtg cgctgcaggc
     4381 ttttcatcac ctcgtcgcgg ctgtcggcgt agttggagta gtcggtctcg ctcatgccgg
     4441 cgaccagcgc gtgcagattg cgtcggaacc cgatcgcggc cgcataagtg aagaacagca
     4501 aattgcggcc gggcacgaag gtcgtcggca ggctcttgtc ggccaggcgg ctgcgtgcat
     4561 cgctggtcaa tgccgtgtgc ccgatctgac tgagcgctgc cagtttgatc aggtgatccg
     4621 gcccgagtcg cccgcgccac tcgggcatgg cgtcgcacag cgccttcagc acctgcggcc
     4681 tgacatcgag ttcgaccgga tgtcgctgcc cgtaatcgaa cccgacggtc tccacgtgcg
     4741 cgtagcgcgt cagcgcccat gccagacaca cggtcgagtc gagtccgccg gaaaacagga
     4801 cgagtgcgcg ctgatcaggg ttcatcggcg tcggaacggt taacccgatt tcaggagtcc
     4861 gctcgttgaa agtctcgtca atcctgctgc gggaggtcga aaatgctgcg cccaagcttt
     4921 gtggcggctg ccattgtcgc ggtactggcc gcgtgcgcca caactgtggt acgcgcgaat
     4981 gcggtcggca gtctggctga tgttcaggtg atggatcgca ccaccggcga aacactgccg
     5041 atctacctgc atcagggtcg tcgctacgtg gccggcacgc ccggcaaccg ctatgcgatc
     5101 gccgtgcgca accgcaccgg cgggcgcctg ctggcggtaa tgtcggtcga cggcgtcaac
     5161 gtcgtcaccg gtgaaaccgc cgcgtgggac cagaacggct acgtcttcga cccgggacag
     5221 cgctgggagg tccgcggctg gcgcaagagc caggagcgca tcgccgcatt cgagttcacc
     5281 gcgttgccaa attcctacgc cgcgcgtacc ggccggccgc agcacgtcgg ggtgatcggt
     5341 gtggcgctgt ttcgcgaggc gaaggccgcg cctgcgccgc tggagccgca gcggcgcgaa
     5401 cctggcggga cgggcgcggg agagaccggt gccgccgtgg cagcgccgga ggccgaccgc
     5461 gccgaacgcc gccgtgacgc gtcccggctc ggcaccggcc acggccgcag cgaaagttca
     5521 ccggtcgcgt tgaccgattt ccagcgccta cgcggcacgc cggacgaggt gatcaccgtc
     5581 cattacgaca gccgcgacaa cctgatcgcg ctcggggtga taccggcggt gcgcgcgccg
     5641 gtgccgaatg cgttccccgg cagcatcggc tttgttcccg atccgccacg gtgaccgccg
     5701 ccgcccgacg ggcccctgcg ccgataatcc ggcgcatgcg cgacgcaccg gccgccgacg
     5761 ttcgcagcga cgagcaactg ctgctcgact acggtgccgg cgaccgggac gcattcgcgc
     5821 ggctgttcga tcggcacgag cgcccggtgt accgcttcct gctgcgctcg ctggggcgcg
     5881 acgcagcgcg cgccgatgac ctgttgcagg aggtatggct ggccgtgatc cgcaacgcgt
     5941 cgacctggga gccgcgcgcg cgcttcacga cctggcttta cgggatcgcg cgttcgcggc
     6001 tgatcgatca ctggcgcgcg cagaagggcg aggtgagcct cgatgcggcc aacgatccgg
     6061 aggccgacga cccgggcgct gcgctgatcg accgcatcgc cggcgcgcgc aacgacgagc
     6121 ccgaggtgcg cgcgatgtcg cgcgcgcagg cacgcgcgtt cgtcgccgcg gtcgaacggc
     6181 tgccggtcgc gcagcgcgag gcgctgctgc tgcacctcga aggagaactg acgctggacg
     6241 aaatcgcgca actgacgggt gtcggcatgg agacggccaa gagccggctc cgctacgcgc
     6301 tcgccaagct gcgcgagtgg ctggaggact ggcgatgacg acccgtcccg atcccgcggc
     6361 cgcctaccgc gccgccaacg ccgcgctcga cgagcgcccg tcggacgcaa
//
LOCUS       DBNHBY010000038         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_949487, whole genome shotgun sequence.
ACCESSION   DBNHBY010000038 DBNHBY010000000
VERSION     DBNHBY010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_949487"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC16HB_00960"
     CDS             <1..406
                     /locus_tag="AC16HB_00960"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR5605385.1"
                     /translation="AIGMLYKLNASISGAEMGCQGEVGVACSMAAAGLVAALNGTNEQ
                     IENAAEIGMEHNLGLTCDPIAGLVQIPCIECNAMGAVKAINAARLSMRGDGTHKVSLD
                     QVIATMRQTGRDMAAIYKETSQGGLAVNVPEC"
     gene            409..1110
                     /locus_tag="AC16HB_00965"
     CDS             409..1110
                     /locus_tag="AC16HB_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5605386.1"
                     /translation="MQGKVVVVTGAFGALGSTVARVFGATGAHMALLDVSQPSAELGQ
                     EFSGAQHFVLGGVDLANTEATRKALTAIAMRFGGIDVLVNVAGGFRWEKVEDGEVDTW
                     DALYTMNLRSAVVCCRAVLPAMLERGAGRIVNVGAGAAAGKAGVGMGAYAASKAGVHK
                     LTEALSEELKDRGITVNAVLPSIIDTPRNRLDMPDADFAKWVTPEQVAQVIRFLASDE
                     AVAVTGALIPISGRS"
     gene            complement(1179..1637)
                     /locus_tag="AC16HB_00970"
     CDS             complement(1179..1637)
                     /locus_tag="AC16HB_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605387.1"
                     /translation="MLNRIRKSLAILATCAIGIVALSTAAVAADDVPVTGGDFWEVTG
                     IHLKDGGSLKYANFLAGEWRANQEFAKSKGWIKSYMVLMNRYPRKGEPDVYLITVSDS
                     IPGGAEQDKRDAEYYAWRKTTATKMEEESGNRAEFRELGSSSLLQEMKFK"
     gene            complement(1771..2343)
                     /locus_tag="AC16HB_00975"
     CDS             complement(1771..2343)
                     /locus_tag="AC16HB_00975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR5605388.1"
                     /translation="MSLGKASKSKLQTLETQRAGRAGKALKRLTLVRHAKSDWSLPGQ
                     EDWDRVLNRRGQQDAPEMARRLRSRKLKPDLILASPAVRALATASIMARELKVPAECI
                     AQDERLYLAEPKAFLDVIRELGGDAAHLMVFGHNPGITETANLLSSGEKIDNLPTCGV
                     FTALFAVDDWSQLDAGSGDDAEFDYPRNLG"
     gene            complement(2340..2657)
                     /locus_tag="AC16HB_00980"
     CDS             complement(2340..2657)
                     /locus_tag="AC16HB_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR5605389.1"
                     /translation="MSTWTRVIPAEEIAPGDYASVEVDGSFVAVFNVGGEFFAIDDVC
                     THDGGGLAGGEIDDHQVICPRHGARFCLRTGQALTPPAYEPVRSYATRIVDGYVEVGS
                     SDT"
BASE COUNT          442 a          903 c          928 g          497 t
ORIGIN      
        1 cgcgatcggc atgctctaca agctcaacgc atcgatctcc ggcgccgaga tgggttgcca
       61 gggcgaggtc ggcgtagcct gctcgatggc ggccgcgggc ctggtcgccg cgttgaacgg
      121 caccaacgag cagatcgaga acgccgccga gatcggcatg gagcacaacc tcggcctgac
      181 ctgcgacccg attgcgggcc tggtgcagat cccctgcatt gaatgcaacg cgatgggcgc
      241 cgtgaaggcg atcaacgccg cgcgactgtc gatgcgcggc gatggcacgc acaaggtgtc
      301 gctcgaccag gtgatcgcga ccatgcgcca gacgggccgt gacatggcgg ccatctacaa
      361 ggagacgagc cagggtggcc tggccgtcaa cgtgccggag tgctgagcat gcagggcaag
      421 gtggtcgtcg tgaccggcgc tttcggcgcg ctcggctcca ctgtggcgcg tgtcttcggc
      481 gcgaccggcg cacacatggc gctgctcgac gtatcgcaac cgtcggccga gctcgggcag
      541 gaattctcgg gtgcgcagca cttcgtgctc ggcggggtcg accttgccaa cacggaggcc
      601 acgcgcaagg ccctcacggc gatcgccatg cgcttcggtg gcatcgacgt gctggtcaac
      661 gtcgcgggcg gctttcgctg ggagaaggtc gaggacggtg aggtagacac ctgggacgcg
      721 ctctacacca tgaacctgcg ctcggccgtg gtctgctgcc gcgccgtcct gccggcgatg
      781 ctcgagcgtg gcgcgggtcg catcgtcaat gtgggggccg gtgccgccgc cggcaaggcg
      841 ggggttggca tgggagctta tgctgcttca aaggcgggcg tgcacaagct gaccgaggcg
      901 ctgtccgaag aactgaagga tcgcggcatc acggtcaacg cggtgctgcc gagcatcatc
      961 gacacgccgc gcaacaggct cgacatgccg gacgccgact tcgcgaagtg ggtgacgccg
     1021 gagcaggtcg cgcaggtgat ccggttcctc gcctcagacg aggccgtggc ggtcactggg
     1081 gcgttgattc caatctcggg tcgcagctga gaccgccagt cgccggtccg ctatggcggg
     1141 gccggcgact gggtcgtttc agtgcagtcg gacctgcttt acttgaactt catttcctgc
     1201 agcagactcg agctgccgag ttcgcggaat tcggctcgat tgccggattc ctcttccatc
     1261 ttcgtggcgg tcgtcttgcg ccaggcgtag tactcggcgt cgcgcttgtc ctgctccgcg
     1321 ccccctggaa tgctgtcgga aacggtgatc aggtagacgt ccggctcgcc cttacgaggg
     1381 tagcggttca tcagcaccat gtagctcttg atccatccct tggacttggc gaattcctgg
     1441 ttggcacgcc attcgccggc gaggaaattg gcgtacttga ggctgccgcc gtccttgagg
     1501 tgtataccgg tgacttccca gaagtccccc ccggtaaccg ggacgtcgtc ggccgcgacg
     1561 gccgccgtcg ataacgccac gatcccgatc gcgcaggtcg caaggattgc gaggctcttc
     1621 ctgatccgat tgagcattgt tcttccccct gacgcggaaa tgcgccgtgt cgcacggtaa
     1681 tcgctttcgc tggaaaaaga atgccgcact gcaagaggac gacacgaatt gaatgctgcg
     1741 ctgcgacccg tcgcccgggc cggcgtcgcc tcagccgaga ttgcgggggt aatcgaactc
     1801 cgcgtcgtcg ccactgccgg cgtccagctg cgaccagtcg tcgacggcga acagggccgt
     1861 gaacacgccg caggtcggca ggttgtcgat cttctcgccg ctcgacagca ggtttgccgt
     1921 ttcggtgatg ccggggttgt ggccgaacac catcaggtgt gccgcatcgc cgccgagctc
     1981 acggatcacg tcgagaaacg ccttgggctc cgcgaggtag aggcgctcgt cctgcgcgat
     2041 gcattcggcc ggaaccttga gttcgcgcgc catgatgctt gccgtggcaa gcgcgcgtac
     2101 ggcgggactg gccagaatga ggtccggctt gagtttgcgc gaccgcagac gccgcgccat
     2161 ctccggcgca tcctgctggc cgcgccggtt gaggacgcga tcccagtcct cctggcccgg
     2221 cagcgaccag tccgacttcg cgtggcgtac gagcgtgagg cgcttcagcg ccttgccggc
     2281 gcggcccgca cgctgcgttt cgagcgtctg cagcttgctc ttgctggcct tgccgaggct
     2341 catgtgtcgc tgctccccac ttcgacgtaa ccgtcgacga tccgcgtcgc gtagctgcgc
     2401 acaggctcgt atgccggcgg cgtcagcgcc tgacccgtgc gcaggcagaa gcgtgcgccg
     2461 tggcgcgggc agatgacctg gtgatcgtcg atctctccgc cagcgagtcc gccgccgtcg
     2521 tgcgtacaca cgtcatcgat ggcaaagaac tcaccgccca cattgaacac ggcgacgaac
     2581 gagccgtcga cctccactga cgcgtagtcg cccggcgcga tctcttccgc gggaatgacg
     2641 cgggtccagg tgctcacgcg cgcgctcaca tcatccccgt ctgcaaccgc gccgcctccg
     2701 acatcatcga ctggttccac ggcggatcga acaccagttc gacgtcggcg cgcttcacgg
     2761 tcggaatgcg
//
LOCUS       DBNHBY010000039         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_949557, whole genome shotgun sequence.
ACCESSION   DBNHBY010000039 DBNHBY010000000
VERSION     DBNHBY010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_949557"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="AC16HB_00985"
     CDS             <1..144
                     /locus_tag="AC16HB_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HIR5605390.1"
                     /translation="AGDIEGKRPGFTDMVGRAKWDAWNEIKGTAANDAMKQYVALVED
                     LKA"
     gene            213..1532
                     /locus_tag="AC16HB_00990"
     CDS             213..1532
                     /locus_tag="AC16HB_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5605391.1"
                     /translation="MRAAIFALALAFGTPLASAGTVTVITSFPKELTSAYKSAFEKRN
                     PGITLEILNKNTVAGIAFVRETAAGKRPEIFWASAPDAFEVLKRDKLLAPAKELANPK
                     VPGKIGNYPINDPEGYYLGQALAGYGIMYNTRYLKANKLAAPQEWNDLLKPQWFGHVA
                     MTSPSRSGTMHLTVETILQGEGWDKGWSTILRMAGNSAQITERSFGVPDGINNGQFGA
                     GPVIDFFGLAGKYSGFPVEFVYPQMTSIVPANIALIANSRNPGEARRFVEFALSQEGQ
                     ELLLTPSISRLPVLPYPAFGGRIPAGYPDPQAIVKRSKVQFNADLSEQRYAIVQSLFD
                     QTITFRLKELQAATKAIIDAEGKLGSRKNAEAEALLKQARDLAYAPVISSQIADQKDF
                     QKLFASNKREVAVAKQITGLEGSWNSKAKANYEKAAEFARQAAALIK"
     gene            1537..>2120
                     /locus_tag="AC16HB_00995"
     CDS             1537..>2120
                     /locus_tag="AC16HB_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605392.1"
                     /translation="MTPTTAHAAVSASGWSATLRHRYGASWGALAVAAAVVLYLAAFL
                     LVPVGTVFVTAFRDADGSLTLGHFGSFFTTALMRESFVNSLFVAGVSVVIASLIAVPL
                     AYITVRFQFRGALLIQTLGVLPLIMPPFVGAVAMTLIFGRSGSVNLLLDEYLGFTLPL
                     MQDLTGVIFVESLHYFPFILMNLTVALRNIDGAM"
BASE COUNT          371 a          693 c          698 g          358 t
ORIGIN      
        1 gccggcgaca tcgaaggcaa gcgccctggg ttcaccgaca tggtcgggcg cgccaagtgg
       61 gacgcatgga acgagatcaa gggcacggcg gccaacgacg cgatgaagca gtacgtcgcg
      121 ctggtcgaag acctgaaggc ttgaagcaag gagcgcggtc cgacggcccg ccgcgcgggc
      181 cgtcgtcgtt ttcggagggg tggagcaaac agatgagagc ggctatcttc gcactggcgc
      241 tggcgttcgg gacaccgctg gcaagcgccg gcaccgtcac ggtcatcact tcgttcccga
      301 aggagttgac cagcgcgtac aagtcggcgt tcgagaagcg caaccccggc atcacgctcg
      361 agatcctcaa caagaacacg gtggcgggca tcgcgttcgt gcgcgagacc gcggccggca
      421 agcggcccga gatcttctgg gccagcgcgc ccgacgcgtt cgaggtcctc aagcgcgaca
      481 agctgctcgc gcccgcgaag gaactcgcca atcccaaggt gccgggcaag atcggcaact
      541 atccgatcaa cgatcccgag gggtactacc tcggccaggc gctggccggc tacgggatca
      601 tgtacaacac ccggtatctg aaggcgaaca agctcgccgc gccgcaggag tggaacgatc
      661 tgctgaagcc gcagtggttc ggccacgtcg cgatgacctc tccgtcgcgc tctgggacga
      721 tgcacctgac ggtggagacg atcctgcagg gcgaaggctg ggacaagggc tggtcgacga
      781 tcctgcgcat ggccggcaac agcgcgcaga tcaccgagcg ttcgttcggc gttccggacg
      841 gcatcaacaa cggccagttc ggcgccgggc cggtgatcga cttcttcggt ctggccggca
      901 agtactcggg cttcccggtc gagttcgtct atccgcagat gacgtcgatc gtgccggcca
      961 acatcgcgct gatcgccaac agccgcaatc cgggcgaggc gcgccgattc gtcgagttcg
     1021 cgctgtcgca ggagggccag gaactgctgc tgacgccgtc gatctcgcgc ctgccggttc
     1081 tgccctaccc cgcattcggc ggccggatcc cggccggcta tcccgacccg caggcgatcg
     1141 tcaagcgcag caaggtgcag ttcaacgccg atctgtccga gcagcgctat gcgatcgtgc
     1201 agtcgctgtt cgaccagacg atcacgttcc ggctcaagga actgcaggcg gcgaccaagg
     1261 cgatcatcga cgccgaggga aagcttggtt cccggaagaa cgccgaggcg gaggcactgc
     1321 tgaagcaggc gcgcgatctc gcatacgcgc cggtgatctc gtcgcagatc gccgaccaga
     1381 aggacttcca gaagctcttt gcgtcgaaca agcgtgaggt cgcggtcgcc aagcagatca
     1441 ccggactgga agggtcctgg aacagcaagg cgaaggcgaa ttacgagaag gcggccgagt
     1501 tcgcaaggca ggcggcggcg ctgatcaaat agccggatga ctcccacgac cgcgcacgcc
     1561 gctgtctccg ccagcggctg gtcggccacg ctgcgccatc gctacggtgc ctcgtggggc
     1621 gcgctggcag tggcggcggc tgtcgtcctt tatctcgccg cgttcctgct ggtcccggtc
     1681 ggcacggtct tcgtcaccgc gttccgcgat gccgacggca gcctgacgct cggccacttc
     1741 ggttcgttct tcacgaccgc gctgatgcgc gagtcgttcg tcaactcgct gttcgtcgcc
     1801 ggcgtcagcg tcgtcatcgc gtcgctgatc gcggtgccgc tggcgtacat caccgtgcgc
     1861 ttccagttcc gcggcgcgct cctgatccag accctcggcg tgctgccgct gatcatgccg
     1921 cccttcgtcg gcgcggtggc aatgacgctg atcttcggcc gctcggggtc ggtcaatctt
     1981 ctgctcgacg aatatctcgg cttcacgctg ccgctgatgc aggacctgac cggcgtgatc
     2041 ttcgtcgaga gcctgcacta ctttccgttc atcctgatga acctgaccgt cgcgctgcgc
     2101 aatatcgacg gcgcgatgga
//
LOCUS       DBNHBY010000040         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_340560, whole genome shotgun sequence.
ACCESSION   DBNHBY010000040 DBNHBY010000000
VERSION     DBNHBY010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_340560"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16HB_01000"
     CDS             complement(<1..299)
                     /locus_tag="AC16HB_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605393.1"
                     /translation="MRKLFRQEAIDAQREKLLGEVSVARPVPLWVFTLLAVAFAGALV
                     AFAFLGEYTRRERVEGFLALDAGAARIQAPEIGTISDLMVKEGDEGAAGAPIARLS"
     gene            complement(359..1336)
                     /gene="senB"
                     /locus_tag="AC16HB_01005"
     CDS             complement(359..1336)
                     /gene="senB"
                     /locus_tag="AC16HB_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HIR5605394.1"
                     /translation="MARPRVAIVTPYLAASNAGNWHTTARWARYLHRHYRVAIMERWD
                     GRPVDCLIALHARRSAESIQKFAAAHPDRPLGVVLTGTDLYRDIAANATAQRSLELAS
                     RLVVLNERGPLRLPRRYRKKTTVIVQSARQLSPARKPHRTFHVAVVGHLREEKNPRLV
                     WRLLETLPADLRLCVHHAGAGLDPALAARARGIAAHDPRYRWLGNLPRAQARQLIRRC
                     HVLLHPSNMEGGAQAVIEAVMSHTPVIGSGIDGNAGLLGVHYAGLFTEGDMEAARALI
                     VRAAREPRFLAQLAWACAKRGKLFTPEREARAVQRLVDNLLREYAARGA"
     gene            complement(1351..>2106)
                     /gene="fabI"
                     /locus_tag="AC16HB_01010"
     CDS             complement(1351..>2106)
                     /gene="fabI"
                     /locus_tag="AC16HB_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5605395.1"
                     /translation="LSNRSIAYGIAQACRREGAELALTYVGERFKERVTEFAQEFGSS
                     IVLACDVGEDAQIASMVEGLRAHWDGLDGLVHSIGFAPREAISGGFLEGLTRENFRVA
                     HDISAYSFPALAKATLPLMQGRRAALVTLTYLGADRVVPHYNTMGLAKASLEASVRYL
                     ATDLGPQGIRVNGISAGPIRTLAASGIKGFSRMLEFNEQNAPLRRNVTIEEVGNVAAF
                     LLSDLASGMTGEITYVDAGFRAIAFGLAAVKGE"
BASE COUNT          323 a          744 c          727 g          312 t
ORIGIN      
        1 gacagccgtg cgatcggcgc accggccgcg ccctcgtcgc cttccttgac catcaggtcg
       61 ctgatggtgc cgatttccgg cgcctggatg cgggctgcgc ccgcgtcgag cgcgagaaat
      121 ccttcgacgc gttcgcgccg cgtgtattcg ccgaggaagg cgaacgcgac cagcgcgccc
      181 gcgaaggcga ccgccagcag cgtgaacacc cacagcggca ccggccgcgc caccgacact
      241 tcgccgagca gcttctcgcg ctgcgcgtcg atggcttcct gccggaatag tttcctcatg
      301 cgtgaatcgt tcgctgcggc cgggatcggt gccgcgcgat tatgcgggaa gccagcgctc
      361 aggcgccgcg ggcggcgtat tcgcgcaaca gattgtcgac cagccgctgc acggcgcgcg
      421 cctcgcgctc gggcgtgaac agcttgccgc gctttgcgca cgcccaggca agctgcgcca
      481 gaaagcgcgg ctcgcgggcc gcgcgcacga tcagcgcgcg cgccgcctcc atgtcgcctt
      541 cggtgaacag cccggcgtaa tgcacgccga gcagcccggc attgccgtcg atgccgctgc
      601 cgatcacggg cgtgtgcgac atcactgcct cgatcaccgc ctgtgcaccg ccctccatgt
      661 tcgacgggtg cagcagcaca tggcagcggc gaatcagctg ccgcgcctgc gcgcgcggca
      721 ggttgccgag ccagcggtag cgcggatcgt gtgccgcgat cccgcgcgcg cgcgccgcca
      781 gcgccggatc aagcccggcg ccggcgtgat gaacgcaaag ccgcaggtcg gcgggcagcg
      841 tctcgagcag ccgccagacc aggcgcgggt tcttctcctc gcgcagatgg ccgacgaccg
      901 cgacgtggaa ggtccggtgc ggcttgcgcg cgggcgacaa ttgccgcgcc gattgcacga
      961 tcaccgtcgt cttcttccgg taccggcgcg gcagccgcag cggaccgcgc tcgttcagca
     1021 ccacgagccg cgaggcgagt tccagcgagc gctgcgcggt cgcgttggcg gcgatgtcgc
     1081 gatacagatc ggtcccggtc agcaccacgc cgagcgggcg atccggatgg gcggcggcga
     1141 acttctggat cgactcggcc gagcggcgcg cgtgcagcgc gatcaggcag tcgaccggtc
     1201 ggccgtccca gcgctccatg atcgcgacgc gatagtgccg gtgcaggtaa cgcgcccagc
     1261 gcgccgtggt atgccagttg ccggcgttcg aagcggcgag gtagggcgtg acgatcgcaa
     1321 cccgcggccg agccatggca gcggctgccc ctattcgccc ttgaccgccg cgagtccgaa
     1381 cgcaatggcg cggaagccgg cgtcgacata ggtgatttcg ccggtcatgc ctgacgccag
     1441 atccgacagc agaaacgcgg cgacgttgcc gacttcctcg atcgtcacgt tccgccgcag
     1501 cggcgcattc tgctcgttga actccagcat gcgactgaag cccttgatgc cgctggccgc
     1561 gagcgtcctg atcggtccgg ccgagatgcc gttgacgcgg atgccctgcg ggccgaggtc
     1621 ggtggcgaga taacgcacgg atgcctcgag cgacgccttg gccagcccca tcgtgttgta
     1681 gtggggcacg acgcggtcgg cgcccagata cgtcagcgtc acgagcgccg cgcggcgccc
     1741 ttgcatcagc ggcagcgtcg ccttggccag cgccgggaag ctgtaagcgg agatgtcgtg
     1801 cgcgacgcgg aagttctcgc gcgtcaggcc ttcgaggaag ccgccgctga ttgcctcgcg
     1861 cggggcgaac ccgatcgaat gcaccagccc gtcgagacca tcccaatgtg cgcgcagccc
     1921 ctcgaccatc gacgcgatct gcgcgtcctc gccgacgtcg cacgcgagca cgatcgagga
     1981 gccgaactcc tgcgcgaatt cggtcacgcg ctccttgaag cgctcgccga cgtaggtgag
     2041 ggcgagttca gcgccctcgc gccggcacgc ctgtgcgatg ccgtaggcga tcgatcgatt
     2101 cgacag
//
LOCUS       DBNHBY010000041         2482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_746667, whole genome shotgun sequence.
ACCESSION   DBNHBY010000041 DBNHBY010000000
VERSION     DBNHBY010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_746667"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="AC16HB_01015"
     CDS             complement(<1..980)
                     /locus_tag="AC16HB_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5605396.1"
                     /translation="MRVPLHRLSATRLAQMIANREVTSEVVTRSFIDAIHARERQVQA
                     FVWFDADRALAIARELDRLQANDARRGPLHGVPVAVKDIIDTADMPTGYGSPIYASHR
                     PPADAAVVALLANAGAFVLGKTVTAELATFTPGLTRNPHNPAHTPGGSSSGSAAAVAA
                     HFAPLALGTQTAGSVIRPAAFCGVAGYKPSPRLVPRAGVKQTSDTLDEVGVFAQSVED
                     LALAASVLALKPAWREASAPAGVPTIAWTATPQSQLASPALRETLERAAQKLLAAGAR
                     HTELTWPRVFDGLFEAHRVVQVYETARALGPEFNYRRDKLSQRLVDMIEQG"
     gene            complement(1321..2058)
                     /locus_tag="AC16HB_01020"
     CDS             complement(1321..2058)
                     /locus_tag="AC16HB_01020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5605397.1"
                     /translation="MSLKGKTIFISGASRGIGLAIAKRCAADGCNVAIAAKTADPNPK
                     LPGTIYTAAKEIETAGGQCLPLQVDIRDELQVVAAAQKTAEKFGGIDIVINNASAINL
                     TNTEATPIKRFDLMFGVNVRGTFLVTQVCLPYLKQSGERKRNPHVLTLSPPLNLEARW
                     FAPHVAYTMAKYGMSMCVLGHAEEFRAYGIAVNALWPRTVIQTAALQMIPGVRPEHCR
                     TPEIMADAAYAVLNLDAAATTGNFFID"
     gene            complement(2055..>2482)
                     /locus_tag="AC16HB_01025"
     CDS             complement(2055..>2482)
                     /locus_tag="AC16HB_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR5605398.1"
                     /translation="GHVHAADVAVVVVGVNDIKKEVPLQQALRKRGEIAAWLRTHVGA
                     SHVVFPATPDMDLFPALPQPLAWLAGKLSKRNNAAQARWARTLPGVSHVAMDGVTQSE
                     LFAADGFHPAPALYARVRARLALHIEAIARAKMTEKEQA"
BASE COUNT          361 a          856 c          856 g          409 t
ORIGIN      
        1 ccctgctcga tcatgtcgac gagccgctgt gacagcttgt cgcggcggta gttgaactcg
       61 ggaccgagcg cgcgcgcggt ctcgtacacc tgcaccacgc gatgcgcctc gaacagtccg
      121 tcgaacacgc gcggccaggt gagctccgtg tggcgcgcgc cggcagccag cagcttctgc
      181 gcggcgcgtt cgagcgtctc gcgcagcgcg gggctggcca gttgcgactg cggcgtcgcc
      241 gtccacgcga tcgtcggcac gcccgcaggc gccgaagcct cgcgccaggc cggcttcagc
      301 gccagcaccg atgcggcaag cgcgaggtcc tcgaccgact gcgcgaatac cccgacctcg
      361 tcgagcgtgt ccgaagtctg cttgacgccc gcgcgcggca cgaggcgcgg cgacggcttg
      421 tagccggcca cgccgcagaa cgccgcgggc cggatgaccg atcccgcggt ctgcgtgccg
      481 agcgccagcg gcgcgaagtg ggccgcaacc gccgccgcgc taccgctcga cgagccgccg
      541 ggcgtgtgcg cggggttgtg cgggttgcgc gtcaatccgg gcgtgaatgt cgccagttcc
      601 gcggtcaccg tcttgccgag cacgaacgca cccgcgtttg cgagcagcgc gacgaccgcg
      661 gcatcggccg gcgggcggtg cgatgcgtag atcggcgagc cgtagccggt cggcatgtcg
      721 gcggtatcga tgatgtcctt gaccgccacc ggcaccccgt gcagcggccc gcgccgcgcg
      781 tcgttggcct gcagccggtc gagttcgcgc gcgatcgcca gcgcgcgatc ggcgtcgaac
      841 catacgaatg cctgcacctg gcgctcgcgc gcgtggatcg catcgatgaa cgagcgcgtg
      901 acgacctcgc tcgtcacctc gcggttggcg atcatctgcg ccagccgcgt ggcggacagg
      961 cggtgcagcg gcactctcat cgcgtgctcc cgctgccggt ccggcgcatt cggctcgcgg
     1021 ctacaacggg cttcggcacg gcctcttcgg cgggcggccg ggcggcggcc gactgcacga
     1081 tcacgaccga cgtcgggatg gcggcgccgg ccgacagcgc ggccgactgc ttgagaaagc
     1141 ggcggcgggc tggaaagcga tttcgctgat tgaccactgc ggactccttg cttggcgcaa
     1201 ggataacccg cggcccgccg acggcctcgt tcagagaaac aggtccggca gcagctgctt
     1261 cgagcccgga acgaccgagt acttcccgag gtcggtcacc ccggcgcgct tcagcacttc
     1321 ctagtcgatg aagaaattgc cggtggtggc cgcggcgtcg aggttcagca ccgcgtaggc
     1381 ggcatcggcc atgatctccg gtgtgcggca atgctcgggc ctgacaccgg gaatcatctg
     1441 cagcgccgcg gtctggatca cggtgcgcgg ccaaagcgcg ttgaccgcga tgccgtaggc
     1501 acggaattcc tcggcgtggc cgagcacgca catgctcatg ccgtatttgg ccatcgtgta
     1561 cgcgacgtgc ggcgcgaacc agcgcgcctc caggttcaac ggcggcgaca gcgtcagcac
     1621 gtgcgggttg cgcttgcgct cgcccgactg cttcaggtag ggcaggcaca cctgcgtgac
     1681 cagaaacgtg ccgcgcacgt tgacgccgaa catcagatcg aagcgcttga tcggcgtggc
     1741 ctcggtgttg gtcagattga tcgcgctggc gttgttgatc acgatgtcga tgccgccgaa
     1801 cttctcggcg gtcttctgcg ccgctgcgac gacctgcagt tcatcgcgga tgtccacctg
     1861 cagcggcagg cactgcccgc cggcagtctc gatctccttc gcagcggtgt agatcgttcc
     1921 cggcagcttc ggattcgggt ccgcggtctt ggcggcgatc gcgacgttgc agccatcggc
     1981 cgcgcatcgt ttggcgatcg ccagtccgat gccgcgcgag gcgccgctga tgaagatcgt
     2041 ctttcctttc aggctcatgc ttgctccttc tcggtcatct ttgctcgtgc gatggcttcg
     2101 atatgtagtg ccagccgcgc acgcacgcgt gcgtacagcg ccggcgccgg atgaaagccg
     2161 tcggcggcaa acagttcgga ctgcgtgacg ccgtccatcg cgacatgcga cacgcccggt
     2221 agcgttcgcg cccagcgcgc ctgcgccgca ttgttgcgct tcgacagctt gccggccagc
     2281 caggcgagcg gctgcggcag cgccgggaag aggtccatgt ccggtgtggc cggaaacacg
     2341 acgtgcgatg cgcccacgtg cgtgcgcagc cacgccgcga tctcgccgcg cttgcgcagc
     2401 gcctgctgca gcggcacttc cttcttgatg tcgttgacgc cgaccacgac caccgcgaca
     2461 tcggcggcat gaacgtgacc at
//
LOCUS       DBNHBY010000042         2737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_273073, whole genome shotgun sequence.
ACCESSION   DBNHBY010000042 DBNHBY010000000
VERSION     DBNHBY010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_273073"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..702)
                     /locus_tag="AC16HB_01030"
                     /pseudo
     CDS             complement(1..702)
                     /locus_tag="AC16HB_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658286.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(724..1278)
                     /locus_tag="AC16HB_01035"
     CDS             complement(724..1278)
                     /locus_tag="AC16HB_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605399.1"
                     /translation="MLVVMGDRASALLARQARDSLNCTVDVGVVGLEVVEKTPAERCD
                     PILADGQPARFDAPWRVRACAIDQPVSIRRTAGTGRQCWQRENLMKDEGKRIDLNEVE
                     RLVEALERDLERARRGEADIGELRGEVEQLRATLAADQVAHADVHRGLLGVRDRLHAL
                     GDELFDDAVKAGDYIARLGRLLGM"
     gene            1369..2046
                     /locus_tag="AC16HB_01040"
     CDS             1369..2046
                     /locus_tag="AC16HB_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR5605400.1"
                     /translation="MGLQTVPTELAVVGWSLFSALIILALRPLAAATARRAFLPHSIQ
                     QHAWLAGMVALGVLWSLPIRVGGGLQFAMIGVSLYALLFGYARALLGSAVALLGLTAL
                     TDGAWASVGLQGLVAIALPAALATALQRLIAANLPRQVFLFIIGNGLFFTLIASGCTS
                     VAMLLLQIALAPGEAPNASELIGYALLLAWGEALASGMIFSALVIFRPQIVMTYTQDD
                     YLPPRGR"
     gene            complement(2049..>2737)
                     /locus_tag="AC16HB_01045"
     CDS             complement(2049..>2737)
                     /locus_tag="AC16HB_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605401.1"
                     /translation="ESRENFADLSLRYLLGSLGMAQVGIGHARTETRLLSGAPGLTVG
                     GGTDGAIARGQLMVDTLDAVGYPRSGYALGGGITYYDGAPGSGARKTAAQLSAALPLT
                     YGRYTLLASVAAATSNQAFGVRYGGLFNLSGTPRGEVAGASGMLVRGVFYRNVSDAFG
                     DIQMPIFGGLSLEAATALAPGEAFAWADFRRAASLFFGAESPVGPLYFALGKTFGGSS
                     ALYFLWGRPN"
BASE COUNT          412 a          961 c          907 g          457 t
ORIGIN      
        1 tggccgatat ggcgcttccg gccgggccat ctcgtcggcc gacagcgcca cgccgagcgg
       61 gagagcggga tcatttcgcg ctcttcctcg cggtaggcga ggttgtagtg gtcctgcatc
      121 gacacgaact tggtcccgcc gcgcgtctcc gccacgtatt gcatgcgcgc gaactgacac
      181 gcaaacatgc tggaggcgcc gatgtagcgc gccttgccgc tcttgaccac atcgggcagc
      241 gcctccatct gtctcctcga ccggcgtctc gtagtcgaag cgatggatct ggtacaggtc
      301 cacgtagtcg acgccgagcc gcttcaggct cgcgtcgatc gcggccagaa tgcgcttgcg
      361 cgacagtccg ctctggttcg gctgcttcgc cccagccggc gcggcgaagc tgcccttaaa
      421 cgcggcgtcg accgggtaga agaccttggt ggcgatcacc acctcgtcgc gcggcgcgaa
      481 gtcgtgcagc agccggccgg tgatctcctc ggacacgccg gcagagtacg tgtcggcagt
      541 gtcgaagaag ttgatgccaa gctcgactgc gcgccgcacg atcggccgtg ccgccgcctc
      601 gtcgagtacc caggcgcgcc actgcggcga gccgtaggtc atcattccga gggcgatccg
      661 cgagaccttc aggccgctcg tgccgaggtt cacgtactgc attgcggagc cctcccgcga
      721 aggctacatg ccgagcaagc gtccgagccg ggcgatgtag tcgccggcct tgaccgcgtc
      781 gtcgaacagc tcatcgccca gcgcgtgcag gcgatcgcgc acacccagca gtccacggtg
      841 cacgtcggca tgtgcgacct ggtcggccgc gagggtggcg cgcaactgtt cgacttcgcc
      901 gcgcagttcg ccgatgtccg cctcgccgcg ccgcgcacgc tcgagatcgc gctccagcgc
      961 ctcgaccagt cgctcgactt cgttgaggtc gatccgcttg ccttcgtcct tcatgagatt
     1021 ctcgcgttgc cagcactgcc gtcccgtgcc ggccgtgcgg cgaatgctaa caggctggtc
     1081 gatcgcgcag gcacggacgc gccacggcgc atcgaagcgt gcgggctgac cgtccgccag
     1141 gatcggatcg cagcgctccg cgggcgtctt ctcgaccact tcgaggccaa cgacaccaac
     1201 gtcgaccgtg caattcagac tatcccgcgc ctgcctggcc agcagcgcgc tggcccggtc
     1261 gcccatgacg acgagcacgc cccaagctcg caggcgccgc gaggcacagc gcgaccgcgc
     1321 cggtcgacaa tgtggatcaa cccacgttgc tgctagcctc ggcttccgat ggggctgcag
     1381 accgttccga ccgaactcgc cgtcgtcggc tggtcgctct tttccgcact gatcattctt
     1441 gcgctgcgcc cgctcgccgc tgcgacggca cgccgcgcgt tcctgccgca ttcgatccag
     1501 cagcacgcgt ggctggccgg aatggtcgcg ctcggcgtgc tgtggtcgct gccgatccgc
     1561 gtcggcggcg gtttgcaatt cgcgatgatc ggcgtatcgc tgtacgcgct cctattcggc
     1621 tatgcgcgcg cgttgctggg cagcgcggtt gcgctgctcg ggctgaccgc gctcaccgac
     1681 ggcgcgtggg caagcgtcgg gctgcaaggg ttggttgcga tcgcgctgcc ggccgcactc
     1741 gcgactgcgc tgcagagact gatagcggcg aacctgccgc ggcaggtgtt cttgttcatc
     1801 atcggcaacg gcctgttctt cacgctgatc gcttcgggct gcacgagcgt ggcgatgctg
     1861 cttttgcaga ttgcgctggc gccgggcgag gcgccgaacg cttctgaact gattggctac
     1921 gcgctgctgc ttgcctgggg tgaggcgctg gcgagcggga tgatcttctc ggcgctggtg
     1981 atcttccgcc cgcagatcgt gatgacgtac acgcaggacg attatctgcc gccgcgtggc
     2041 cgctgacctc agttcggccg gccccacagg aagtacagcg cgctcgagcc cccaaacgtc
     2101 ttgccgagcg cgaagtacag cgggccgacg ggagattcgg caccgaagaa cagcgacgcc
     2161 gcgcgccgga aatccgccca cgcgaacgcc tcgccgggcg cgagcgcagt cgcggcctcg
     2221 agcgacagac cgccgaagat cggcatctgg atgtcgccga acgcgtctga aacattgcgg
     2281 tagaacacgc cgcgcacgag catcccgctc gcgcccgcga cctcgccacg cggcgtgccg
     2341 gagaggttga acagcccccc gtagcgcacg ccgaaggcct ggttcgatgt cgccgccgct
     2401 acgctcgcga gcagcgtgta gcgtccgtaa gtcagcggca gcgccgcgct caactgcgct
     2461 gcagtcttgc gcgcgccgct gccgggcgcg ccgtcgtagt acgtgatgcc gccgccgaga
     2521 gcgtagcccg agcgcggata gccgacggcg tcgagcgtgt cgaccatgag ctgcccgcgc
     2581 gcgatcgcac cgtcggtgcc gccgcccaca gtcagccccg gagcgccgct gagcagccgc
     2641 gtctcggtac gcgcatggcc gataccgacc tgcgccatgc caaggctgcc cagcagatag
     2701 cgcagcgaaa ggtccgcgaa gttctcgcgc gattcga
//
LOCUS       DBNHBY010000043         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1017189, whole genome shotgun sequence.
ACCESSION   DBNHBY010000043 DBNHBY010000000
VERSION     DBNHBY010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1017189"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..774
                     /locus_tag="AC16HB_01050"
     CDS             25..774
                     /locus_tag="AC16HB_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease family protein"
                     /protein_id="HIR5605402.1"
                     /translation="MVWQSIDTVPLDLGTGIAPSRAYAIAQALTRRVLPLVRLLDGRP
                     EPLATAALFVVDGRVLLLTCKHALEQGVSIGDLGVPLGTDAIFWLRVAAPRVLAHPAR
                     DLACIALAPRAAALLLVHWRAAPLRVDRAPAAARTLAVVGYPYAQMRRHAHVMYARPL
                     VMFTRGQAIGSAGDLRLCYGRVAVRVDGVEIHTPPLDGVSGATCWAVHDEDGDRCVLQ
                     PIAVQIAFKHDEYARAEPVHAAAPLIAILRA"
     gene            complement(778..1350)
                     /locus_tag="AC16HB_01055"
     CDS             complement(778..1350)
                     /locus_tag="AC16HB_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5605403.1"
                     /translation="MESWPRILIVDSHGQPVRWAVLARAARYRAAGKVITDLGENLFA
                     MTGGTQESSGRRSEFITSSIVMIRGRHRIPLGYAHVGLTKHRLFARDRQICGYCGGHF
                     AEGDLTVEHILPVSRGGQHDWTNVVTACRSCNTRKGNRTPEEAHMPILYVPYRVCRNE
                     GFILSNRRILADQMAFLQATLPRHSRWAHG"
     gene            complement(1430..1654)
                     /locus_tag="AC16HB_01060"
     CDS             complement(1430..1654)
                     /locus_tag="AC16HB_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605404.1"
                     /translation="MQTKLDQIARERAVLALLRELAALGLREGDAVRNVESGEVGRLA
                     VTRTDDAPRAVVTLPAGGQAAFDDRWQRFG"
     gene            complement(1744..2268)
                     /locus_tag="AC16HB_01065"
     CDS             complement(1744..2268)
                     /locus_tag="AC16HB_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605405.1"
                     /translation="MPMHAPSGAIGRAIAVAIVFVLAGCALPTAVAPGTPEVDVIGRY
                     GRPAEVYPLPSPPQARRLAYPLGRFNQQTWLVDVDAEGRVLRVVEAISAEHFAQIRVG
                     QDDTTSVRREFGPPRVIRTYTLSGLTAWMYPYLEAGWWNSEMAVYFDKSGIVTKVESG
                     PDPRFVGGSDGKPH"
     gene            2308..2838
                     /locus_tag="AC16HB_01070"
     CDS             2308..2838
                     /locus_tag="AC16HB_01070"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5605406.1"
                     /translation="MKWLVRTIAGLIILVVAFLAVGFVLPSHFRIERSATINAPAERV
                     YSMLVAPSEWKRWTVWNQRDPAMKVDYSGPPSGVGARWSWESESEGNGRMEFTEALPN
                     QRIGYKLLFPEFGMQSAGALTIEPAGSGVRVSWTNEGEMGTNPVNRWFGLLMDRMVGK
                     DFEAGLANLKKLAEGG"
     gene            complement(2847..3032)
                     /locus_tag="AC16HB_01075"
     CDS             complement(2847..3032)
                     /locus_tag="AC16HB_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605407.1"
                     /translation="MKRIWLLLPVGLLSAGCAILGPAEGARYEVDSERVAAVEHAARQ
                     AGVTVHWVSYPRKKVAD"
     gene            3179..>3430
                     /locus_tag="AC16HB_01080"
     CDS             3179..>3430
                     /locus_tag="AC16HB_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605408.1"
                     /translation="MTRASAALVVTTAIQVAVALAALTLPAIAPVVARDLGVPTSMIG
                     SYLSALYLGASAAALVGGGLVLRLGAIRLSQWGLVLCAAV"
BASE COUNT          516 a         1170 c         1196 g          548 t
ORIGIN      
        1 cgggaaattc gatagcctgt ttccatggtc tggcagtcga tcgacaccgt tccgctcgac
       61 ctcggcaccg gcatcgcgcc gtcgcgcgcc tatgcgatcg cgcaggcctt gacgcgccgc
      121 gtgctgccgc tggtgcgcct gctcgacggc cgccccgagc cgctggcgac ggcggcgctg
      181 ttcgtggtcg acggccgcgt gctgctgctg acctgcaagc acgcgctcga gcagggcgtg
      241 tcgatcggcg acctcggcgt gccgctcggt accgatgcaa tcttctggct gcgcgtcgca
      301 gcgccgcgcg tgctcgcgca tccggcgcgc gatctcgcgt gcatcgcact ggctccgcgc
      361 gctgccgcgc tgctgctcgt gcactggcgc gcggcgccgc tgcgcgtcga tcgcgcaccg
      421 gcagcggcgc ggacgctggc cgtggtgggc tacccgtacg cgcagatgcg gcgccatgcg
      481 cacgtgatgt acgcgcggcc gctggtgatg ttcacgcgcg gccaggcgat cggaagcgca
      541 ggcgacctgc gcttgtgcta cggccgcgtg gccgtgcgtg tcgacggggt cgagatacac
      601 acgccgccgc tagacggcgt ctcgggcgcg acctgctggg cggtgcacga cgaggacggc
      661 gatcgctgcg tgctgcagcc gatcgccgtg cagatcgcgt tcaagcacga cgaatacgcg
      721 cgtgccgaac ccgtgcacgc ggccgcgccg ctgatcgcaa ttctccgggc ctgagactca
      781 accgtgggcc cagcgcgagt ggcgcggcag cgtggcctgc aggaacgcca tctggtcggc
      841 gaggatgcgc cggttgctga ggatgaaccc ttcgttgcgg cacacgcgat agggcacata
      901 caggatcggc atgtgcgctt cttccggcgt gcggttgccc ttgcgcgtat tgcacgagcg
      961 gcaagcggtc acgacattgg tccagtcgtg ctgcccgccg cgcgacaccg gcagaatgtg
     1021 ctcgaccgtc aggtcgcctt cggcaaagtg tccgccgcag taaccgcaga tctgccggtc
     1081 gcgcgcgaac aggcggtgct tcgtcagtcc gacgtgtgca tagccgagcg gaatgcggtg
     1141 ccgcccgcgg atcatcacga tcgaactggt gatgaactcc gagcgccgcc ccgaggactc
     1201 ctgcgtgccg ccggtcatcg cgaacaggtt ttcgcccaga tcggtgatca ccttgccggc
     1261 ggcgcggtag cgcgccgcgc gcgccaggac cgcccaacgg accggctggc cgtgggagtc
     1321 gacgatcagg atgcgaggcc aggactccaa ggcgaacatg tcgcaagttt gccacgacag
     1381 ttcgcgattt gcatgagccg ggcagcctaa ccggttgccc gcaaagggct tatccgaagc
     1441 gttgccagcg gtcgtcgaac gccgcctgcc cgccggcggg cagagtgacg acggcgcgcg
     1501 gcgcgtcgtc ggtgcgggtc accgcgagcc tgccgacttc gcccgactcg acgttgcgca
     1561 cagcgtcgcc ttcgcgcaga cccagcgcgg cgagttcgcg cagcagcgcg agcaccgcgc
     1621 gctcgcgggc gatctggtcg agcttggtct gcaacgactg catccaagcg atgttgcgag
     1681 cgcgcgcgcg gccccgccat cgggacttgc ccggtccgtc gggaaacccc cgatgcgacg
     1741 acttcagtgc ggcttgccgt cgctgccgcc gacgaagcgc ggatcggggc ccgattcgac
     1801 tttcgtgacg atgcccgact tgtcgaagta gaccgccatc tcggagttcc accagccggc
     1861 ctcgagatac ggatacatcc acgccgtgag ccctgacaac gtgtaggtgc ggatcacgcg
     1921 cggcggtccg aattcgcgcc gaacgctggt cgtgtcgtcc tgaccgacac ggatctgcgc
     1981 gaaatgctcg gccgaaatcg cctcgacgac gcgcagcacg cggccttccg catcgacgtc
     2041 gaccagccag gtctgctggt tgaagcggcc gagcggatac gcgagccgcc gcgcctgcgg
     2101 cggcgaaggc aacggataca cctcggccgg ccggccgtag cggccgatca cgtcgacctc
     2161 gggcgtgccg ggtgccaccg cagtcggcag cgcacatccg gccaggacga acacgatcgc
     2221 aaccgcgatt gcgcggccga tcgcgccact gggtgcatgc atcggcatac tgtcgctccc
     2281 tgctaacaat aatcgcggag acgggcaatg aaatggcttg ttcgcacgat cgccgggctg
     2341 atcattctgg tcgtcgcatt cctggcagtc ggcttcgtgc tgccgtcgca tttccgcatc
     2401 gagcgctctg ccaccatcaa cgccccggcg gagcgggttt attccatgct ggtcgcgccg
     2461 agcgaatgga agcgctggac agtgtggaac cagcgtgacc cggcgatgaa ggtcgactac
     2521 agcggaccgc cttcgggcgt tggcgcacgc tggtcgtggg aaagcgagtc cgaaggcaac
     2581 ggcaggatgg aattcaccga ggcgctgccg aatcagcgga tcggctacaa acttttgttt
     2641 cctgaattcg gcatgcagtc ggccggcgcg ctgacgatcg agccggccgg cagcggcgtg
     2701 cgcgtcagtt ggaccaatga gggcgagatg ggtaccaacc cggtcaaccg ctggttcggc
     2761 ctgctgatgg accggatggt cggcaaggac ttcgaggccg gactcgccaa cctgaagaag
     2821 ctggccgagg gcggctgatg gccgacctag tcggcgacct tcttccgcgg gtaactgacc
     2881 cagtgcaccg tcacccccgc ctggcgtgcg gcgtgctcca ccgcggcgac ccgttcgctg
     2941 tccacctcgt accgcgcacc ctcagcagga ccgaggatcg cgcatccggc ggacagcagc
     3001 ccgaccggca gcagcaacca tatgcgcttc atcgcattct cccgccggtt gcagcgaacg
     3061 caaccgcgat cctgaatcta cgccgcagcg cgccgcttgc aagcgtgcgg cgacggggcc
     3121 ggcaatttcg ggaacaggac ggcgccgggc ggccggcggc ggcgccgctg cgcggccgat
     3181 gacgcgtgcg agcgcggcgc tcgttgtcac gactgcgatc caggtcgcgg tcgcgcttgc
     3241 ggcgctgacg ctgccggcga tcgcgccggt cgtcgcccgc gatctcggcg tgccgacgtc
     3301 gatgatcggc agctacctgt cggcgttgta tctcggcgcc tcggccgcgg cgctggtcgg
     3361 cggcggtctc gtgctgcggc tcggcgcgat ccggctgtcg cagtggggac tcgtgctgtg
     3421 cgcggccgtg
//
LOCUS       DBNHBY010000044         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_746858, whole genome shotgun sequence.
ACCESSION   DBNHBY010000044 DBNHBY010000000
VERSION     DBNHBY010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_746858"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC16HB_01085"
     CDS             <1..227
                     /locus_tag="AC16HB_01085"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permuted SH3 domain-containing protein"
                     /protein_id="HIR5605409.1"
                     /translation="LKTARTLGNEWLVDSGLKSGDRVIVDGLQRVRPGMVVQAVERGD
                     PNAAPAPPGTPPAAAGAAPATTKSGTTPGS"
     gene            250..>2540
                     /locus_tag="AC16HB_01090"
     CDS             250..>2540
                     /locus_tag="AC16HB_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605410.1"
                     /translation="MSRFFIERPIFAWVIALVIMLAGLLSIFRLPIAQYPNIAPPTIT
                     ISTTYPGASAKTVEDTVTQIIEQRMTGLDGLRYITSTSDSFGSATITLTFNSGVDPDI
                     AQVQVQNKLKLAEVLLPQDVQQQGVTVTKSVRNFIMVIGFVSEDGSLTRTDISDYVAA
                     NVLDPLSRVPGVGEITLFGSQYAMRIWLDPNKLINYKLTPADVAAAIRAQNAQVSAGA
                     LGGLPAVPGQQLNATITAQSRLQTAEQFDNIVLRTAESGATVRLRDVARAELGGENYN
                     TVSFYNGRPSSGVAVRLATGANALDTGDAVKVKVDELSRFFPQGLKAVVAYDTTPFIR
                     LSIHEVVITLVEAFVLVFLVMYLFLQNLRATLIPSIAVPVVLLGAFGVMAAFGFSINV
                     LTMFGLVLAIGLLVDDAIVVVENVERVMSEEGLSPKEATKKSMGQITGALVGIALVLA
                     SVFIPMAFFGGSTGVIYRQFSLTLAASMLLSVTVALILSPALCATLLKPVAKGSHGAT
                     AGFFGWFNRNFDRGRANFQRVAAGMINRPRRWLAAFVAMVALAAFLAYRLPTSFLPDE
                     DQGILFTQVLLPAGATQERTVAVLREIERHFLQDEKDAVQSLFTVAGFSFSGSGQNAG
                     IGFVRLKDWKDRPGAQNSVQAVARRAMAAFSKLRDALAFAFAPPAVLELGVANGFDMY
                     LQDRGGAGHEALMAARNQLLGLAAKEPALTAVRPNGQEDTPQFQVDIDTVRAGALGLS
                     IADVNTTLSAAWGSAYVNDFIDKG"
BASE COUNT          389 a          844 c          872 g          435 t
ORIGIN      
        1 cgctgaagac cgcgcgcacg ctcggcaacg agtggctggt cgattcgggt ctgaagtccg
       61 gcgaccgcgt gatcgtcgac ggcctgcagc gcgtgcgccc cggcatggtc gtgcaggccg
      121 tcgaacgtgg cgatcccaac gccgcgccgg caccacccgg cacgccgccc gcggccgcag
      181 gcgccgcgcc ggcgacgacg aagtccggca ccacgccggg ttcctgaccg cagccgtcgg
      241 gagccatcga tgtctcgctt cttcatcgaa cgaccgatct ttgcctgggt gatcgcgctc
      301 gtgatcatgc tggccgggct gctgtcgatc ttccgcctgc cgattgcgca gtacccgaac
      361 atcgcgccgc cgacgatcac gatctcgacc acatatcccg gcgcgtcggc caagacggtc
      421 gaggacacgg tcacccagat catcgaacag cgcatgaccg ggctggacgg cctgcgctac
      481 atcacctcga ccagcgacag cttcggctcg gcgacgatca cgctgacctt caattcgggc
      541 gtggatcccg acatcgcgca ggtgcaggtg caaaacaagc tgaagctcgc cgaggtgctg
      601 ctgccgcagg acgtgcagca gcagggtgtg accgtcacca agtcggtgcg caacttcatc
      661 atggtgatcg gtttcgtgtc ggaagacggc tcgctgacgc gaaccgacat ctcggactac
      721 gtggccgcca acgtgctgga tccgctgtcg cgcgtgccgg gcgtgggcga gatcacgctg
      781 ttcgggtcgc agtacgcgat gcgcatctgg ctcgacccga acaagctgat caactacaag
      841 ctgacgccgg ccgacgtcgc cgccgcgatt cgcgcacaga acgcgcaggt gtcggccggc
      901 gcgctgggcg ggctgcccgc ggtaccgggc cagcagctca acgcgacgat caccgcccag
      961 agccgactgc agacggccga gcagttcgac aacatcgtgc tgcgtacggc cgagagtggc
     1021 gccaccgtgc ggctgcgcga cgtggcgcgc gccgaactcg gcggcgagaa ctacaacacc
     1081 gtttccttct acaacggccg gccctcgtcc ggcgtggcgg tccggctggc cacgggcgcc
     1141 aacgcgctcg acacgggcga tgcggtcaag gtcaaggtcg acgagctgtc gcgcttcttt
     1201 ccgcaggggc tgaaggcggt cgtggcctac gacacgacgc cgttcatccg tctgtcgatc
     1261 cacgaggtgg tgatcacact ggtcgaagcg ttcgtgctgg tgttcctcgt gatgtacctg
     1321 ttcctgcaga acctgcgcgc gacactgatc ccgagcattg cggtgccggt cgtgctgctc
     1381 ggcgcgttcg gcgtgatggc ggcgttcggc ttctcgatca acgtgctgac catgttcggt
     1441 ctcgtgctcg cgatcggcct gctggtcgac gacgcgatcg tcgtggtgga aaacgtcgag
     1501 cgcgtgatga gcgaggaggg cctgtctccc aaggaggcga ccaagaagtc gatgggccag
     1561 atcaccggcg cgctggtcgg catcgcgctg gtgctggcgt cggtgttcat cccgatggcg
     1621 tttttcggcg gctccaccgg cgtgatctat cgccagttct cgctgacgct ggcggcgtcg
     1681 atgctgctgt cggtgaccgt cgcgctgatc ctgtcgccgg cgctgtgtgc gacgctgctc
     1741 aaacccgtcg cgaaggggtc acatggcgcc accgccggct tcttcggctg gttcaaccgc
     1801 aacttcgatc gcggccgcgc gaacttccag cgcgtggcgg ccggcatgat caatcgaccg
     1861 cgtcggtggc tggctgcgtt cgtagcgatg gtcgcgctcg ccgcctttct cgcctatcgc
     1921 ctgccgacgt cgttcctgcc cgatgaagat cagggcatcc tgttcaccca ggtgctgctg
     1981 ccggccggcg caacgcagga gcgcacggtg gccgtgctgc gcgagatcga acggcacttc
     2041 ctgcaggacg agaaggacgc cgtgcagtcg ttgttcacgg tggcgggctt ctcgttttcc
     2101 ggcagcgggc agaacgccgg catcggcttc gtgcggctca aggattggaa ggaccgcccg
     2161 ggcgcgcaga actcggtgca ggcggtggcg cggcgcgcga tggcggcgtt ttccaagctg
     2221 cgcgacgccc ttgcgttcgc gttcgcgccg cccgcggtgc tcgagctcgg cgtggccaac
     2281 ggcttcgaca tgtacctgca ggaccgtggc ggcgcgggtc acgaggcgct gatggccgcg
     2341 cgcaatcagc tgctcgggct ggcggccaag gagccagcac tgacggcggt gcggcccaac
     2401 ggccaggagg acacgccgca gttccaggtc gacatcgata ccgtgcgcgc cggtgcgctc
     2461 gggctttcga tcgccgacgt caacacgacg ctgtcggcgg cgtggggcag cgcctacgtg
     2521 aacgacttca tcgacaaggg
//
LOCUS       DBNHBY010000045         3431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1829628, whole genome shotgun sequence.
ACCESSION   DBNHBY010000045 DBNHBY010000000
VERSION     DBNHBY010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1829628"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..415)
                     /locus_tag="AC16HB_01095"
     CDS             complement(101..415)
                     /locus_tag="AC16HB_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605411.1"
                     /translation="MPEFLYLLINYPALIAIPVVAFTALAVWSGSRTAWVATVAWVAY
                     LGYEIGMGAGVFCSGENCIRRPELYLVYPLLAVLSLVALVQTYVHVREKHRRERPATL
                     RR"
     gene            complement(511..1428)
                     /locus_tag="AC16HB_01100"
     CDS             complement(511..1428)
                     /locus_tag="AC16HB_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5605412.1"
                     /translation="MADASGGSLRAIFFALGANTGIAITKFGAAAYTGSGAMLAEAIH
                     SVADCANQVLLLVGMRQARRPESAEHPFGHHRVVYFWSMMVALLLFFVGGAFSMYEGI
                     ERLRHPEPLKNALVALAVLAAAVALEAVSLWGALREIRKVQARRTFWRWFRETRQSEL
                     MVVAGEDIAALLGLVFAFVAIAAAMATGNPALDAAGSIAVGLLLMMVAVAVLLEVKAM
                     IVGESAEPAVRAAIRAHVEARPEVRHVINLLTLQWGEKLIVAVQAEMNDPGSAAALIA
                     AVNAVEASIQQAFPQARWVFFEPDVRRRA"
     gene            complement(1433..1864)
                     /locus_tag="AC16HB_01105"
     CDS             complement(1433..1864)
                     /locus_tag="AC16HB_01105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR5605413.1"
                     /translation="MRVLPALLCLAASTGVLAQSDPLSAAAAEKGAVKTESGLIFRAL
                     KEGNGASPVATDTVRVHYRGTFPDGKEFDSSYARKAPAEFPLNRVIKCWTEGVQKMKV
                     GGRAKLTCPPAIAYGEHGAGGVIPPNATLQFEIELLAIVNK"
     gene            1939..3267
                     /locus_tag="AC16HB_01110"
     CDS             1939..3267
                     /locus_tag="AC16HB_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR5605414.1"
                     /translation="MADLFESNPADAPLAERMRPRALVEVIVQAHLLGPGKPLRVAFE
                     SGKPHSMILWGPPGTGKTTLARLMADAFGLDFIAISAVLGGVKDIRDAVERAQLNRSQ
                     SGKPTVVFVDEVHRFNKAQQDAFLPHVESGLFVFVGATTENPSFEVVGALLSRAAVYV
                     LEPLGDSDLDQLIERALARHLPKLAVDADARRVLIALADGDARRLLNALEIAATGARE
                     QGQHEIDAAFLRATLPATLRRFDKGGENFYDQISALHKAVRGSDVDAALYWMTRMLDG
                     GVDPHYVARRAIRMATEDIGLADPRALRLALDAAEAFERLGSPEGELALAEAIVYLAF
                     APKSNAIYTAFGAARAFVKEDSSRPVPLRLRNAPTRLMKELGYGEGYRYAHDEAGAVA
                     AGEQYLPDGMAAPNWYEPTERGLEARIRERMEELRRLNQSTRSAARRHKP"
BASE COUNT          559 a         1197 c         1176 g          499 t
ORIGIN      
        1 tgctggcgtt caagagcttc gagcgcaacc cgaagctcga cgccgggctg ttccagttcg
       61 tcccgcccaa gggtgcggac gtgctcgagg accgatagcg tcagcgccgc agcgtcgccg
      121 gccgctctcg gcggtgcttt tcgcgcacgt gcacgtaggt ctgcaccagc gccaccagcg
      181 acaggacggc cagcagcgga tagaccagat acagctccgg gcgtcggatg cagttttcgc
      241 cgctgcagaa cacccccgca cccatgccga tctcgtagcc gaggtatgcg acccaggcca
      301 ccgtggcaac ccacgcggtg cgcgaaccgc tccacaccgc aagcgcagtg aacgccacca
      361 ccggaatcgc gatcagcgcc ggatagttga tcagcaggta aaggaactcg ggcatcggtc
      421 ggcgccgcgg ttggcgttgg cggccgtcgg gcgcgcgcta tcgtaccccc ggaccggcgc
      481 gcgagacaca ggcggcgcca cggtggatgc ttatgcgcga cgccgcacgt cgggctcgaa
      541 gaacacccag cgcgcctgcg gaaatgcctg ctggatcgat gcctcgacgg cgttgacggc
      601 ggcgatcagc gccgcggccg agccgggatc gttcatttcc gcctgcacgg cgacaatcag
      661 cttctcgccc cactgcagcg tcagcaggtt gatcacgtgc cgcacctcgg gccgcgcctc
      721 gacgtgcgcg cggatggcgg cgcgcaccgc gggctcggcc gattcgccca cgatcatcgc
      781 cttgacctcc agcagcaccg cgaccgcgac catcatcagc agcagcccga ctgcgatcga
      841 gcccgccgcg tcgagcgccg ggttgccggt cgccatcgcc gcggcgatgg cgacgaacgc
      901 gaacacgagg ccgagcagcg cggcgatgtc ctcgccggcg acgaccatca gttccgactg
      961 gcgcgtctcc ctgaaccagc gccagaaggt gcgtcgggcc tgcaccttgc ggatctcgcg
     1021 cagtgcgccc cacagcgaca ccgcctccag cgcaaccgcc gccgcgagca cggcgagcgc
     1081 gaccagcgcg ttcttcagcg gctcggggtg ccgcagccgc tcgatccctt cgtacatcga
     1141 gaacgcgccg ccgacgaaga acaacagcag cgcgaccatc atcgaccaga agtagaccac
     1201 gcgatggtgg ccgaacggat gctcggcaga ttcgggccgc cgcgcctgcc gcatgccgac
     1261 cagcagcagg acctggttgg cgcagtcggc gaccgagtgg atcgcctcgg ccagcatcgc
     1321 gccggagccg gtgtaggccg cagcgccgaa cttggtgatc gcgatgccgg tgttggcgcc
     1381 gagcgcaaag aagatcgcgc gcagcgagcc gcctgaagcg tccgccacgg cgctacttgt
     1441 tcacgatcgc cagcagttcg atctcgaact gcagcgttgc gttgggcgga atcacgccgc
     1501 cggcgccgtg ctcgccgtac gcgatcgcgg gcgggcaggt gagtttcgcg cgcccgccga
     1561 ccttcatctt ctgcacgccc tcggtccagc acttgatcac gcgattcagc gggaattcag
     1621 ccggcgcttt gcgcgcatac gagctgtcga actccttgcc gtccggaaac gtgccgcggt
     1681 agtgcacgcg cacggtgtcg gtggcgaccg ggctggcgcc gttgccttcc ttcaacgcgc
     1741 gaaagatgag gccggattcg gtcttgaccg cgcctttctc cgcggcggca gcggagagcg
     1801 gatcggattg cgcgagcacg ccggtcgaag cggccagaca cagcagggcg ggcagaactc
     1861 tcatcatggc gctccgttcg attcgcgatg ggcggacgga tgctagcctg aagcacgaca
     1921 atcgcagaaa tcccgtcgat ggccgacctg ttcgagtcca accccgccga cgcgccgctc
     1981 gcggagcgga tgcggccgcg cgcgctcgtc gaggtgattg ttcaggcgca cctgctcggg
     2041 ccgggcaaac cgctgcgcgt cgcgttcgag tcgggcaagc cgcactcgat gatcctgtgg
     2101 ggcccgcccg gcaccggcaa gaccacgctg gcgcggctga tggccgacgc gttcgggctc
     2161 gacttcatcg cgatctcggc ggtgctcggc ggcgtaaagg acatccgcga cgcggtcgag
     2221 cgcgcgcaat tgaaccgctc gcagtcgggc aagccgaccg tcgtgttcgt cgacgaggtg
     2281 caccgcttca acaaggcgca gcaggacgcg tttctgccgc acgtcgagtc gggcctgttc
     2341 gtgttcgtcg gtgcgaccac cgagaaccca tcgttcgagg tcgtcggcgc gctgctgtcg
     2401 cgggcggcgg tgtatgtgct ggagccgctc ggcgacagcg acctcgatca actgatcgag
     2461 cgtgcgctgg cgcggcacct gccgaaactc gcggtggacg ctgatgcgcg gcgcgtgctg
     2521 atcgcgctcg ccgacggcga cgcgcgccgg ctgctcaacg cgctggagat cgctgcgacc
     2581 ggcgcgcgcg agcagggcca gcatgagatc gacgcagcgt tcctgcgtgc gacgctgccc
     2641 gcgacgctgc gccgcttcga caagggtggc gagaacttct acgaccagat ctcggcgctg
     2701 cacaaggcgg tgcgcgggtc ggatgtcgac gcggcgctgt actggatgac gcgcatgctc
     2761 gacggcggag tcgatccgca ttacgtcgcg cggcgcgcga tccgcatggc caccgaagac
     2821 atcgggctgg cggacccgcg cgcgctgcgg ctcgcgctcg acgctgccga ggcattcgag
     2881 cggctgggct ccccggaggg cgaactcgcg ctcgcggagg cgatcgtgta cctcgcgttc
     2941 gcgccgaagt cgaatgcgat ctacaccgcg ttcggcgcgg cgcgcgcgtt cgtgaaggag
     3001 gacagttccc gcccggtgcc gctgcggctg cgcaacgcgc cgacgcggct gatgaaggaa
     3061 ctcggctacg gcgagggtta tcgctacgcg cacgacgagg cgggcgcggt cgccgcaggc
     3121 gagcagtacc tgcccgacgg catggccgcg cccaactggt acgaaccaac cgagcgcgga
     3181 ctcgaggcgc gcatccgcga gcggatggag gaactgcggc ggctgaatca atcgacgcga
     3241 tccgccgcgc gccggcacaa gccgtgagca tcccgcgctt gcctcgcgtg tacggagcga
     3301 cgaaaatcgc aggatgctcg cattcatcct tcaactgtcc gccgcgcatc gcgcgcgaag
     3361 tcgccgcgtc tgaagcacct ggcccgcgcg cgcgagccga ccttcccatt tcgatccgat
     3421 gaaccactcc g
//
LOCUS       DBNHBY010000046         3930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1423326, whole genome shotgun sequence.
ACCESSION   DBNHBY010000046 DBNHBY010000000
VERSION     DBNHBY010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1423326"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC16HB_01115"
     CDS             <1..248
                     /locus_tag="AC16HB_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5605415.1"
                     /translation="VFGDPDEFRLDRDPALNLLYGAGIHVCPGAPLARLELRVVMEEL
                     LARTRDIALVAGKAATKAMYPASGFSDLPLWIAPRTE"
     gene            complement(624..1241)
                     /locus_tag="AC16HB_01120"
     CDS             complement(624..1241)
                     /locus_tag="AC16HB_01120"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5605416.1"
                     /translation="MQAMLDAAPPRSVDTTVAPATAALAAPCFDMLALAFSADPGVRW
                     LFPDAQRYRTHFAPFAHAFGGRAFALGTATHVDKLAAALWLPPDTSPDEEALIGVIER
                     GTPDDRRAQAFEVLEEMGRCHPDEPHWYLPLIGTDPAAQGRGLGSALLAHTLALCDAQ
                     RLPAYLEATSERSVPLYQRHGFDIIAVLRVGTCPPIVPMLRRPRA"
     gene            complement(1201..2565)
                     /locus_tag="AC16HB_01125"
     CDS             complement(1201..2565)
                     /locus_tag="AC16HB_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5605417.1"
                     /translation="MRDSAGESVSGATAASLEAYEQACRELRCFTDDPVASVARALTA
                     SPEMTMAHALKAWLHLLGTEPAGLPVARQCLHVAMALPANERERGHLEAIRRVLDGQW
                     RVAGRVLEDVSAAFPRDALALQAGHQIDFFTGDSRMLRDRVARVLPAWSASLPGYHAV
                     LGMYAFGLEECSDYAAAERYGRDSVARERRDGWGWHAVAHVMEMCNRTADGIAWMTSD
                     TDAWSRASFFAVHNWWHLALFHLDRGEIDEVLRLFDGPIHGARSGVVLDLIDAAALLW
                     RLHLRGVDVGPRWQTVADGWAPVAGAGNYAFNDWHAMMAFVGSGRPKAQQALLGSLTA
                     AADGSGDSAAFVREVGLDAARAIEAFGQQRYADTVRLLRPLRHYAHRFGGSHAQRDLI
                     DLTLIEAALRAGMQPLAQALAAERRARHPESPLARLFVQRAHSTSQENTNAGNARRSA
                     AALG"
     gene            2686..3639
                     /locus_tag="AC16HB_01130"
     CDS             2686..3639
                     /locus_tag="AC16HB_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5605418.1"
                     /translation="MDALSDLLRVLRFSGGVFLDATFRAPWCVTSQVTEEDCAPGLQA
                     TGLIAFHYVLDGRLDVRVGTAAPRNCRAGDLIVIARNDKHLVGSDLGLPPLAAEALIR
                     KASEHDLAQIDFGDGASPLSRFVCGYLAAAVDTSLLLDALPPLLVVHVRERPCAEWVE
                     SSFRYAARAHAARQAGAQDLLARLSELLFVEAVRETMERLPREATGWLAALRDPPLAR
                     ALAAMHARAAHPWTTEQLAEEALLSRSAFAERFARTLGVPPMTYLTRWRMLLACRRLR
                     DSPAPIARVAAEIGYESESTFTRAFTREIGVAPGAYRRQAH"
     gene            3672..>3930
                     /locus_tag="AC16HB_01135"
     CDS             3672..>3930
                     /locus_tag="AC16HB_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5605419.1"
                     /translation="MTDIVLEGVSKRYGAVRAVDDISLRADSGKFLVLLGPSGCGKST
                     VLRLIAGLEEPTAGRILIDGRDVTRLNPDKRRVSMVFQSYAL"
BASE COUNT          547 a         1407 c         1398 g          578 t
ORIGIN      
        1 ccgtgttcgg cgaccccgat gaatttcgcc tcgaccgcga tccggcgctg aacttgttgt
       61 atggcgccgg catccatgtc tgccccggcg cgccgctggc gcggctggag ttgcgtgtcg
      121 tgatggaaga actgctggcg cgcacgcggg acatcgcgct ggtggccggg aaggcagcta
      181 ccaaggcgat gtatcccgcc agcggatttt ccgacctgcc actgtggatc gccccgcgaa
      241 cggaatgagg gccgagggat cgacggcgcc accttgcgga acgcacgggc ctcggcgtcg
      301 aggagacggc cgcgcaccgg cgcaatgcgg tcgaccagag cgaggtcgaa gtgggattgc
      361 cgccgatgac gggatgtact gcagcggcag ccagaaccgc gttcacggcg ctggcttcgg
      421 ggcgattgca gtcgtgtacg cgaatgccgg cagtctcccg gccgccgtcg cagcgacgag
      481 gatcgatgca tcgtgcagtg ctcgctgccg gccgcaccgg gcggcgcgat ccgatcgaag
      541 tgcggacctc tcgcggagat tctcatgcga gctgtttcga cctgcgcgcc gggcgagcgc
      601 cgctgcgcgc gccgcgtacg cgctcacgcg cgcggccgcc gcagcatcgg cacgatcggc
      661 ggacaggtgc cgacgcgcag cacggcgatg atgtcgaacc catgccgttg gtacaacggc
      721 acgctgcgct cgctggtcgc ttccaggtac gcaggcaggc gctgcgcgtc gcacagcgcc
      781 agtgtgtgcg ccagcaatgc cgagccgagc ccgcgcccct gcgcggcggg gtcggtgccg
      841 atcagcggca ggtaccagtg cggctcgtcg ggatggcagc gacccatttc ctcgaggacc
      901 tcgaacgcct gtgcacggcg gtcgtcgggc gtgccgcgct cgatcacccc gatcagtgcc
      961 tcctcgtccg ggctcgtatc cggcggcagc cacagcgcgg cggccagctt gtcgacgtgc
     1021 gtcgccgtcc cgagcgcgaa ggcgcggcca ccgaacgcgt gcgcgaacgg tgcgaagtgc
     1081 gtgcgataac gctgtgcgtc ggggaagagc caccgcacgc ccggatcggc gctgaacgcc
     1141 agcgccagca tgtcgaaaca aggcgccgcc agcgcggctg tggccggtgc gacggtcgtg
     1201 tcaaccgagc gcggcggcgc tgcgtcgagc attgcctgca ttcgtgttct cctgcgaagt
     1261 cgagtgcgcg cgctgcacga acagccgcgc caacgggctc tcgggatggc gcgcacgacg
     1321 ctcggcggcg agcgcctgcg ccagcggctg catgccggcg cgcagcgccg cctcgatcag
     1381 cgtgaggtcg atcaggtcgc gctgcgcgtg gctgccgccg aagcggtgcg cgtagtggcg
     1441 cagcggccgc agcagccgga ccgtgtccgc gtagcgttgc tggccgaacg cctcgatggc
     1501 gcgcgccgcg tcgagaccga cctcgcgtac gaatgccgcg ctgtcgccgc tcccgtcggc
     1561 ggccgcggtc agcgacccga gcagcgcttg ctgcgccttc ggccggccgc tgccgacgaa
     1621 ggccatcatc gcgtgccagt cgttgaacgc gtagttgccg gcgcccgcga ccggcgccca
     1681 gccgtcggcg acggtctgcc agcgcgggcc cacgtcgacg ccgcgcaggt gcagccgcca
     1741 cagcagcgcc gcagcgtcga tcaggtcgag caccacgccg gagcgcgcgc catggatcgg
     1801 cccgtcgaag aggcgcagta cctcatcgat ttcgccgcgg tcgaggtgga acagcgccag
     1861 gtgccaccag ttgtgcacgg cgaagaagct cgcgcgcgac caggcgtccg tgtcgctcgt
     1921 catccaggcg atgccgtcgg cggtgcggtt gcacatttcc atcacgtgcg cgaccgcgtg
     1981 ccagccccag ccgtcgcgcc gctcgcgcgc aacgctgtcg cggccgtagc gctcggcggc
     2041 cgcgtagtcg ctgcactctt ccagcccgaa tgcgtacatg ccgagcaccg cgtggtagcc
     2101 gggcagcgac gcgctccacg ccggcagcac gcgcgcgacg cgatcgcgga gcatgcgtga
     2161 atcgccggtg aagaagtcga tctggtgccc cgcctgcagg gcgagcgcgt cgcggggaaa
     2221 cgcggcgctc acgtcctcca gcacgcgacc ggccacacgc cactggccgt cgagcacgcg
     2281 ccggattgcc tccaggtgcc cgcgctcgcg ctcgttggct ggcagcgcca tcgccacgtg
     2341 caggcactgg cgcgcgaccg gcagccccgc cggctcggtg ccgagcaggt gcagccacgc
     2401 cttgagcgcg tgcgccatcg tcatttcggg actggccgtg agcgcccgag ccacgctcgc
     2461 gaccgggtca tcggtgaagc agcgcaactc gcggcacgcc tgctcgtacg cttccaggct
     2521 cgcggccgtt gctccgctga ccgactcgcc cgcagaatct cgcatcgtca tgaactcctt
     2581 ctcctcgtcg gattgccggc tcgaccggca cgggcgaaag tgtcggccgg ccgccggccg
     2641 cgcgccatgg ccgcggcgcc acgcgtcttt gccgggacac cgctgatgga cgcgctgtcc
     2701 gacctgctgc gcgtgctgcg cttttctggc ggcgtattcc tcgacgccac ctttcgcgcg
     2761 ccgtggtgcg tgacttcgca ggtcaccgag gaggactgcg cgcccgggct gcaggcgacc
     2821 ggtctgatcg cgttccatta cgtgctcgac ggacggctcg acgtgcgcgt cggcaccgca
     2881 gcgccacgca actgccgtgc cggtgacctg atcgtgatcg cgcgcaacga caagcacctg
     2941 gtcggcagcg acctcgggtt gccgccgctg gccgccgagg cgctgatccg caaggcaagc
     3001 gagcacgacc tggcgcagat cgacttcggc gacggcgcct cgccgttgag ccgcttcgtc
     3061 tgcggctatc tggccgccgc ggtcgacacc agcctgctgc tcgacgcgct gccgcccctg
     3121 ctggtcgttc acgtgcgcga gcggccgtgc gcagagtggg tcgaaagctc gttccgctat
     3181 gcggcgcgcg cgcacgcggc acggcaggcc ggcgcgcagg acctgctcgc gcggttgtcc
     3241 gaactgctgt tcgtcgaggc ggtgcgcgaa acgatggagc gactgccgcg cgaggcgacg
     3301 ggttggctcg cggcgctgcg ggacccgccg ctcgcacgtg cgctcgccgc catgcacgcg
     3361 cgcgccgcgc atccgtggac caccgagcag ctggcagaag aggcgctgct gtcgcgctcg
     3421 gcattcgccg aacgcttcgc acgcacgctc ggcgtgccgc cgatgacgta tctgacgcgc
     3481 tggcgcatgt tgctcgcctg ccggcggctg cgtgactcgc cggcgccgat cgcgcgcgtc
     3541 gcggccgaga tcggctacga atccgaatcc acgttcacgc gcgcattcac gcgcgagatc
     3601 ggtgtggcgc ccggcgcata ccggcgccaa gcgcactgag cggccgcttt cgcctgcata
     3661 atccgcgcgc catgaccgac atcgtcctcg agggagtgtc caagcgctac ggtgcggtgc
     3721 gcgcggtcga cgacatcagc ctgcgcgccg actccggcaa gttcctggtg ctgctcggac
     3781 cgtcgggctg cggcaagtcg acggtgctgc ggctgatcgc gggactcgaa gagccgaccg
     3841 cgggccggat cctgatcgac ggccgcgacg tgacacgcct gaatcccgac aagcgccgcg
     3901 tatcgatggt gttccagtcg tatgcgctgt
//
LOCUS       DBNHBY010000047         2478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1152673, whole genome shotgun sequence.
ACCESSION   DBNHBY010000047 DBNHBY010000000
VERSION     DBNHBY010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1152673"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="AC16HB_01140"
     CDS             complement(<1..806)
                     /locus_tag="AC16HB_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5605420.1"
                     /translation="MTQQQPPDSDSDHPRPQRIETIAIHAGREPDAATGAVREPIHLA
                     TTFERAADGSYPHGYSYTRAGNPNRTALERAIAALEHGREAVAFASGSAATLGIFALA
                     APGGRIVCGADCYHGTAKQLRDIVPRWGVDVEFVDTTDLAAVAAALATPTSLLWFETP
                     SNPLLHVSDITALVALARERGTLVGCDNTFASPVLQRPLLLGADVVMHSSTKYLGGHS
                     DVLGGVAVVREPGKLLDGLRDYQGTGGGVPSPFDCWLILRSLPTLPLRVRA"
     gene            complement(803..>2478)
                     /locus_tag="AC16HB_01145"
     CDS             complement(803..>2478)
                     /locus_tag="AC16HB_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5605421.1"
                     /translation="NLALTVHAVLGSLRKWPDSHAGKTLTRVADAALDGARGNSGAIL
                     AQFFLGLGDRLGHLHQLEPDDFVAGVDGGAHYARESLSEPREGTILTVLTDFAHAVQR
                     ARREGLRDFRALLKRGVQAAQVSLEQTTYQLEALRRANVVDAGAQGFVELMSGVMEYI
                     DSGADDAPDVPEHAVVLDASGETTAGAEQDLDYRYCTECVVIAERVDRRHLREQLSTL
                     GGSLVVAGLHNKVRVHIHVNDPAEVFRVAGRFGTVSGEKADDMQRQQHAAHTAGRKVA
                     VVTDSAADIPEDELDRLGIHMVPVRVHFGERSYLDKVGITPEEFYGEIERGQIPKTSQ
                     PPPGDFRRQFEFLGTHFGAVVSVNLSSRVSGTCGAAQTAATRVATHGKVTVIDSGTAS
                     VGQGLVAMYAAECAAAGYDAERVIAATRAIMPRTHVYGMVGSLEYAVRGGRVPRWVQR
                     LADALQLMPVLHADRHGRVTAGGVLFGRRNLKQKFARWVRRRMRDDCTYKLLVAHGNC
                     EADGRELLRVLSAENVGYATLLPVGSALGAHGGPGMLVVGLQEYEAPAP"
BASE COUNT          357 a          868 c          877 g          376 t
ORIGIN      
        1 gcacggaccc gcagcggcag cgtcggcagg ctgcgcagaa tcagccagca gtcgaaaggc
       61 gagggcacgc cacccccggt cccctggtag tcgcgcaatc cgtccagcag tttgcccggc
      121 tcgcggacca ccgcgacgcc gcccagcacg tcactgtggc cgcccaggta cttggtggag
      181 ctgtgcatca cgacgtctgc gccgagcagc agcggacgct gcagcacggg gcttgcaaag
      241 gtgttgtcgc aaccgaccag cgtgccgcgc tcgcgtgcga gcgccaccag tgcggtgatg
      301 tcgctgacgt gcagcagcgg attcgacggt gtctcgaacc acaacaggct ggtcggcgtg
      361 gcgagcgccg ccgcgaccgc ggcaagatcg gtcgtgtcga cgaattcgac gtcaacgccc
      421 cagcgcggca cgatgtcgcg gagctgcttg gccgtgccgt gataacagtc ggcgccgcag
      481 acgatgcgtc cgcccggtgc tgccagcgcg aagatgccga gcgtggcggc gctgccggag
      541 gcgaaggcga cggcttcgcg gccgtgctcg agcgccgcga tggcgcgttc cagggcagtg
      601 cgattcgggt tcccggcgcg cgtgtacgag tagccatgcg gatagctgcc gtcagcggcg
      661 cgctcgaagg tggttgcgag atgaatcggc tcgcgcaccg cgcccgtcgc cgcatcgggc
      721 tcgcgtcccg cgtggatggc gatggtttcg atgcgctgcg gtcggggatg gtcggagtcg
      781 ctgtcggggg gctgctgctg agtcacggcg ccggcgcctc gtattcctgc aggccgacga
      841 cgagcatacc cggcccaccg tgtgcgccga gcgcactgcc cacgggcagc agcgtcgcgt
      901 agccgacgtt ctccgcgctc agcacacgca gcagttcgcg tccgtccgcc tcgcagttgc
      961 cgtgcgcgac cagcaacttg tacgtgcagt cgtcgcgcat gcggcggcgc acccagcgcg
     1021 cgaacttctg tttcagattg cggcggccga acagcacacc acctgccgtg acgcggccgt
     1081 ggcggtcggc gtgcagcacc ggcatcagtt gcagagcgtc agcgagccgc tgcacccagc
     1141 gcggcacgcg tccgccgcgc accgcgtact ccagcgagcc caccattccg tagacgtggg
     1201 tgcgcggcat gatcgcgcgc gttgccgcga tgacgcgctc ggcgtcatag cccgcggccg
     1261 cgcactcggc cgcgtacatc gcgacgaggc cctggccgac cgaggcggtg ccactgtcga
     1321 tcaccgtgac cttgccgtgc gtcgccacgc gcgtcgcagc cgtctgcgcg gccccgcagg
     1381 taccgctcac ccggctgctc aggttcaccg agacgaccgc gccgaagtgc gtgccgagga
     1441 attcgaactg gcggcggaaa tcgcccggcg gtggctgcga ggtcttgggt atctggccac
     1501 gctcgatctc gccgtagaac tcctccggcg tgatgcccac cttgtccagg taactgcgtt
     1561 cgccgaaatg cacgcgcacc ggcaccatgt ggatgccgag ccggtcgagt tcgtcttccg
     1621 gaatgtccgc tgcggagtcc gtgacgacgg cgaccttgcg ccccgcggtg tgcgccgcgt
     1681 gctgctggcg ctgcatgtcg tcggccttct cgccgctgac cgtgccgaag cggcccgcga
     1741 cgcgaaagac ctcggcggga tcgttgacgt ggatgtgcac gcggaccttg ttgtgcaggc
     1801 cggcgacgac gaggctgccg ccgagcgtcg agagctgctc gcgcaggtgc cggcggtcga
     1861 cgcgctccgc aatgacgacg cactccgtgc agtagcggta atcgaggtcc tgctccgcac
     1921 ccgccgtcgt ctcgcccgag gcgtcgagca ccactgcgtg ctccggcacg tccggcgcgt
     1981 cgtcggcgcc gctgtcgatg tactccatca cgcccgacat cagctcgacg aagccctgcg
     2041 cgccggcatc gacgacattg gcccggcgca gcgcctcgag ctggtacgtg gtctgctcca
     2101 gcgaaacctg cgccgcctgg acgccgcgct tcagcagcgc gcggaaatca cgcaggccct
     2161 cgcggcgggc gcgctgcacg gcgtgcgcga agtcggtcag taccgtgagg atcgtgcctt
     2221 cgcgcggctc gctgaggctt tcgcgcgcgt aatgcgcgcc gccatcgacg cctgcgacga
     2281 agtcgtcggg ttcgagctgg tgcaggtgac cgagccggtc accgagcccg aggaagaact
     2341 gcgccaggat cgcgccggaa ttgccgcggg caccgtcgag ggcggcgtcc gccacgcgcg
     2401 tcagcgtctt gccggcgtgg ctgtcaggcc acttgcgcag ggacccgagc acggcgtgca
     2461 cggtcagcgc gagattgg
//
LOCUS       DBNHBY010000048         1639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_408444, whole genome shotgun sequence.
ACCESSION   DBNHBY010000048 DBNHBY010000000
VERSION     DBNHBY010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_408444"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /gene="rho"
                     /locus_tag="AC16HB_01150"
     CDS             <1..831
                     /gene="rho"
                     /locus_tag="AC16HB_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5605422.1"
                     /translation="KRLRLERGTGSTEDLTARMVDLIAPIGKGQRGLIVSPPKAGKTM
                     LLQNIANSITANHPEVYLIVLLIDERPEEVTEMSRTVRGEVVSSTFDEPASRHVQVAE
                     MVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFG
                     AARNIEEGGSLTILATALIDTGSRMDDVIYEEFKGTGNSEIHMDRRIAEKRVFPALNI
                     NRSGTRREELITSAEELQKIWILRKLLHPMDELAAAEFLIDKMRDTKANAEFFEAMKR
                     "
     gene            877..>1639
                     /locus_tag="AC16HB_01155"
     CDS             877..>1639
                     /locus_tag="AC16HB_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5605423.1"
                     /translation="MLAAVASFAAMDSLLKLLVAHYPSLQVAFVRGVAALPFVLAPIL
                     LRRRLARLRPVNLRLHLFRAALSIVMIYSFVSAVRDSSLAATYAIFMCAPLLVAAGSA
                     RLLGERVERAQWVAIAVGFAGVLLMLRPGPGQLGKAGALWALIAVLTYTISVVTLRVL
                     ARTDTNESMVFWFTLALAIGAGLLAIPGWVPLRAEHLTLMLGVGAFGALGQYLITVAF
                     RAAPAAVVTPFEYTALVWGVALDVAIWSVWPNAVTL"
BASE COUNT          255 a          542 c          558 g          284 t
ORIGIN      
        1 aagcgcctgc gcctcgagcg cggcaccggc agcaccgagg acctcaccgc ccgcatggtc
       61 gacctgatcg caccgatcgg gaaaggccag cgcggcctga tcgtttcgcc gccgaaggcc
      121 ggcaagacga tgctgctgca gaacattgcc aactcgatca ccgccaacca tcccgaggtg
      181 tacctgatcg tgctgctcat cgacgagcgg cccgaggaag tgaccgagat gtcgcgcacc
      241 gtgcgcggcg aggtggtgtc gtccacgttc gacgagccgg cgagccgcca cgtgcaggtc
      301 gccgagatgg tgatcgaaaa ggccaagcgt ctcgtcgagc acaagaagga cgtcgtgatc
      361 ctgctcgact cgatcacgcg gctggcgcgc gcctacaaca ccgtcgtgcc gagctccggc
      421 aaggtgctca cgggcggtgt cgacgcgaac gcgctgcagc gcccgaagcg cttcttcggc
      481 gcggcgcgca acatcgagga aggcggcagc ctgacgatcc tggccacggc gctgatcgac
      541 accggttcgc gcatggacga cgtgatctac gaagagttca agggcaccgg caacagcgaa
      601 atccacatgg atcgccgcat cgccgagaag cgcgtgttcc cggcgctgaa catcaaccgc
      661 tcgggcacgc ggcgcgagga actgatcacc tcggccgagg agctgcagaa gatctggatc
      721 ctgcgcaagc tgctgcatcc gatggacgag ctggccgcgg ccgagttcct gatcgacaag
      781 atgcgggaca ccaaggccaa cgccgagttc ttcgaggcga tgaagcgctg acgattgggc
      841 gccccacagg atcatcgcct gcggggaatc gtcgcgatgc tggccgccgt ggccagtttc
      901 gcggcgatgg attcgctgct caagctgctg gttgcgcact atccatcgct gcaggtggcg
      961 ttcgtgcgcg gcgtggctgc gctgcccttc gtcctcgccc cgatccttct gcgtcgccgc
     1021 ctggcgcgcc tgcgtccggt caacctgcgg ttgcacctct tccgcgccgc gctgtcgatc
     1081 gtgatgatct actcgttcgt ctcggcggta cgcgattcct cgctcgctgc gacgtacgca
     1141 atcttcatgt gcgccccgct gctggtggcg gccggttcgg cgcgactgct gggcgagcgc
     1201 gtcgaacgcg cccagtgggt cgcgatcgca gttggtttcg ccggggtgct gctgatgctg
     1261 cgaccgggcc cgggccagct cggcaaggca ggcgcgcttt gggcgctgat cgcggtgctc
     1321 acatacacga tatccgtggt gacgctacgc gtgctggcgc gtaccgacac caatgagagc
     1381 atggtgttct ggttcacgct cgcgctcgcc atcggcgcgg ggctgctggc gatccctggc
     1441 tgggtgccgc tgcgggccga gcacctgacg ctcatgctcg gcgtcggtgc gttcggcgcg
     1501 ctgggccagt acctcatcac ggtcgcattc cgggctgcac cggccgccgt cgtcactccg
     1561 ttcgagtaca ccgcgctggt ctggggcgtg gcattggacg tcgcgatctg gagcgtgtgg
     1621 cctaacgcgg tgacgctgg
//
LOCUS       DBNHBY010000049         2254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1491715, whole genome shotgun sequence.
ACCESSION   DBNHBY010000049 DBNHBY010000000
VERSION     DBNHBY010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1491715"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16HB_01160"
     CDS             <1..497
                     /locus_tag="AC16HB_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-keto acid cleavage family enzyme"
                     /protein_id="HIR5605424.1"
                     /translation="FRNTFKDIENILTTCAQNGTRFEIECYDIGHLYTLRHFADRGLV
                     KPPFFIQSVFGILGGIGPHPEDVAHMKRTADRLFGDQYQWSVLGAGRNQLPIATQSVA
                     LGGNVRVGLEDSLWIGAGKLAESNAQQVRAARQVIEGLGLPVATPDEAREILQLKGRT
                     NVNF"
     gene            508..1269
                     /locus_tag="AC16HB_01165"
     CDS             508..1269
                     /locus_tag="AC16HB_01165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5605425.1"
                     /translation="MSDLQGKVVLVTGASTGIGAAVARAFGREEARVAVHYHVSEAAA
                     QQVVADIRVAGAEAVALRADARDSKQVEKLVADTVAHFGRIDVLVNNAGDIVQRCHVA
                     ESTDELLSSILDLNVRSVVTASRAAIAHFRRQGGGNIINTTSVAARHGGGPGAVLYAA
                     SKGFVSTFTRGLAKEHAKESIRVNGVAPGVIATPLQERHTSAQQMEAFRKSIPMDRLG
                     TPEECVGAYLYLASDALSGYMTGQILEVNGGQLMP"
     gene            complement(1282..1551)
                     /locus_tag="AC16HB_01170"
     CDS             complement(1282..1551)
                     /locus_tag="AC16HB_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605426.1"
                     /translation="MHPVRNPADLAHPGAARLRVAARRRAAGRESESPAAGTQIKGGG
                     GGVRVVAQAAHTDVTLRDVTIAQRSGPESQKLACSGFTASVTIAP"
     gene            1634..2119
                     /locus_tag="AC16HB_01175"
     CDS             1634..2119
                     /locus_tag="AC16HB_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605427.1"
                     /translation="MARGCGPFLQLMAAVQKDRLSVAAQPTTIRTRRPVRFPISQEVP
                     QMSIRTIASRGHAVVFAGALCIAAPAMVFAASPLPPVKVFSDHYEAAGKRFANLDQLE
                     AWVKATGARGLVFHGCMSGPTQPLVAAMERFRNIYLDVRWSLAGEAGCPSEKSMTQAQ
                     R"
BASE COUNT          378 a          768 c          766 g          342 t
ORIGIN      
        1 tcttcaggaa cacgttcaag gacatcgaga acatcctgac gacctgcgcg cagaacggca
       61 cgcggttcga gatcgagtgc tatgacatcg gccacctgta cacgctgcgc cacttcgccg
      121 accgcgggct ggtcaagccg ccgttcttca tccagtcggt gttcggcatc ctcggcggca
      181 tcggcccgca tccggaggac gtcgcgcaca tgaagcgcac cgcggaccgc ctgttcggcg
      241 accagtacca gtggagcgtg ctcggcgccg gccgcaacca gttgccgatc gccacgcaat
      301 cggtcgcgct cggcggcaac gtgcgcgtcg ggctggagga ttcgctgtgg atcggcgcgg
      361 gcaagctcgc cgagagcaac gcgcagcagg tgcgcgcggc gcggcaggtc atcgagggcc
      421 tgggactgcc ggtcgcaaca ccggacgagg cgcgcgagat tctgcagctg aagggccgca
      481 ccaacgtgaa cttctgaccc gtccatgctg agcgatctgc agggcaaggt cgtgctggtc
      541 accggcgcca gcaccgggat cggcgcggcg gtcgcgcgcg cgttcgggcg cgaagaggcg
      601 cgcgtggccg tgcactatca cgtgagcgag gcggcggcgc agcaggtcgt ggccgacatc
      661 cgcgtcgcgg gcgccgaggc ggtcgcgctg cgcgccgacg cgcgcgactc gaaacaggtt
      721 gaaaagctcg tcgccgacac ggtcgcgcac ttcggccgca tcgacgtgct ggttaacaat
      781 gccggcgaca tcgtgcagcg ctgccatgtc gccgagagca cggacgagct gctgtcgtcg
      841 atcctcgatc tgaacgtgcg ttcggtcgtg accgccagcc gcgcggcgat cgcgcacttt
      901 cgcaggcagg gcggcggcaa catcatcaac accacatcgg tcgccgcgcg ccatggcgga
      961 ggaccgggcg cggtgctgta cgccgcatcg aagggtttcg tcagcacctt cacgcgcggg
     1021 cttgccaagg aacacgcgaa ggagagcatc cgcgtcaacg gcgtggcgcc cggcgtcatc
     1081 gcgacgccgc tgcaggagcg gcacaccagc gcacagcaga tggaagcgtt ccgcaagtcg
     1141 atcccgatgg atcgcctcgg cacaccggag gaatgcgtcg gcgcgtacct gtatctcgcc
     1201 tccgacgcgc tctccggcta catgaccggc cagatcctcg aagtcaacgg cggacagctg
     1261 atgccgtgac gcgcacgcct ctcacggcgc gatggtgacg ctcgccgtga agccgctgca
     1321 cgccagtttc tgactctccg gacccgagcg ctgagcgatc gtgacgtcgc gcagtgtcac
     1381 gtcggtgtgc gccgcctgag ccacgacccg gacgccgcca ccgccgccct tgatctgcgt
     1441 gccggctgca ggcgactcgg actcgcgacc cgctgcgcgc cttcgcgcgg cgactcgtag
     1501 tcgagccgca ccggggtgtg cgaggtcggc cgggttccga accggatgca cgcggtctcg
     1561 tgcgagtgca ggcgtgcagc ccggaaaact caggcaaccg ttcggaaccc gcgcaagtgg
     1621 tcgagcgata tgcatggcgc gcggctgcgg tccattcctt caactcatgg cagcggtgca
     1681 aaaagatcga ttgagcgtcg cagcgcagcc cacaacaatt cggactcgtc gtccggtccg
     1741 tttcccgatc tcacaggagg tgccgcaaat gagcattcgc acgatcgcct cacgaggcca
     1801 cgcagtcgta ttcgcgggcg cgctttgcat cgcggcgcct gcgatggtct tcgccgcgag
     1861 cccgctgcca ccggtgaagg tgttttccga tcactacgaa gccgccggca agcgctttgc
     1921 caacctcgac cagctcgaag cctgggtcaa ggcgacgggc gcgcgcggac tcgtcttcca
     1981 cggctgcatg tccggtccga cccagccgct ggtggcggcc atggaacgct tccgcaacat
     2041 ctatctggac gtccgctggt cgcttgccgg cgaggcggga tgtccgagcg aaaagtcgat
     2101 gacgcaagcg cagcgctgat cacccgcagc gcgcggcgaa cgcgagtgca gcgccgcgct
     2161 atgcggccgc ggtcgcctgc gcagcgagtc ggtaaccggc cgccagtgcc tcgacgttca
     2221 gttgcacgaa gctgcgcggc gtatccgcga gcac
//
LOCUS       DBNHBY010000050         5111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_950106, whole genome shotgun sequence.
ACCESSION   DBNHBY010000050 DBNHBY010000000
VERSION     DBNHBY010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_950106"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2060
                     /locus_tag="AC16HB_01180"
     CDS             <1..2060
                     /locus_tag="AC16HB_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HIR5605428.1"
                     /translation="IGRKKLLNILQARCEAVGVKLVFEREVDADTEFPDADLIVASDG
                     INSKIRTMYAENFRPDIVTRPNRYIWLGTNKLYDAFTFLFEKTEHGWFQAHIYRFDET
                     TSTFIVECPEKVWLAHGLDRADQAQSIEFCERLFANNLRGEKLMTNARHLRGSAWLNF
                     QRVKCEQWSHFNGRSHVVLMGDAVHTAHFAIGSGTKLALEDAIELTAQFQRLGDTRDR
                     IAEVLARYQEIRAVDVIRLQNAAWNAMEWFEVCGERYCDQLEPEQFMYSMLTRSQRIS
                     HENLRLRDKGWLEGFERWFAERNASVDPRAGIGAALAVPPMFTPYTVRSVRLKNRVVV
                     SPMAQYSCVDGVPGEFHLVHLGARALGGAGMVVAEMTCTSPDARITPGCPGLWNETQR
                     DAWQRIVDFVHRETDAKIAMQLGHAGAKGSTNAPWDGSGADYPLPADNWSLISASAVP
                     YLDDGPVPRAMTLADMQRVRGDFVRAARHAAEAGFDWLELHCAHGYLLSAFISPLTNR
                     RRDEYGGALANRLRFPLEVFRALREVWPKQLPMSVRISAHDWVDGGITPDDAVEIAKA
                     FKAAGADMIDCSSGQVSPHQRPTYGRMYQTPFADRIRNEAGIATIAVGAIFEADHVNS
                     IVAAGRADLCAIARPHLANPAWTLTEAAKIGYTAIAWPKQYRQAKQQMESNFARDKAA
                     RAA"
     gene            2065..3465
                     /locus_tag="AC16HB_01185"
     CDS             2065..3465
                     /locus_tag="AC16HB_01185"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR5605429.1"
                     /translation="MRKRLLLRHADVLATMNDAGDEIRDGALLIDDQRIVWVGASDDL
                     DPRLAEGAEAIDTRGCVVVPGLVNCHHHLFQTLTRAIGTGRGLRLFDWLKFLYPIWAE
                     LTPEAVYVSAKVGLIELVQSGCTTVADHLYLYPNGTRIDDEIRAAAEVGVRFHPTRGS
                     MSLGASRGGLPPDRICEAEDAILADSVRAIEVFHDPAPLSMLRVGLAPCSPFSVTAAL
                     MRESARLARGHPRVHLHTHLAETRDEDRYCLQMFGKRPLEYAESVEWIGSDVWFAHMV
                     HPDDAEITRLAHTRSGVCHCPSSNMILASGIAPVRAMLDRGVRVGLGVDGSASNDGSH
                     LLGEARQALLLQRVGWPGFESRADRFSAREALRLATRGGAQVLGREDIGVLAPGIAAD
                     VVAFRVDDLSHAGARGDAVAALVTCAPAQAWLTVIDGRVVVRDGIYLGAELPPLVERH
                     NALAREMLERAGVRSG"
     gene            complement(3457..4677)
                     /locus_tag="AC16HB_01190"
     CDS             complement(3457..4677)
                     /locus_tag="AC16HB_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5605430.1"
                     /translation="MSHGALDGWIDAHFDEEVRFLQELVRVPTDTPPGNNAPHAERTA
                     ELLHGMGLEAERHPVPESLVRDHGLISVTNLIVRRRYGEGTIIALNAHGDAVPPGEGW
                     THDPYGAQIVDGKLFGRGAAVSKSDIATYTFAVRALDALGTRPKGTVELHFTYDEEFG
                     GLLGPAWLLQQGLTKPDLLIAAGFSYEVVTAHNGCLQFEVTLHGVASHAAYPETGVDA
                     LQAANRLLSALYEYNDVLRRRRSRIAGITHPYLNVGRIDGGTNTNVVPGKVVLKLDRR
                     MIPEEDSAAVEAEVRGRIERTLTPCPGIRIELKRLLLANALKPLAGNAPLVAAIQKHG
                     QAIFGEPIPTSGTPLYTDARLYGERGVPAVIYGAGPRTVLESNAKRADEHLVLEDLRR
                     ATKVFARTLLDLLA"
     gene            complement(4674..>5111)
                     /locus_tag="AC16HB_01195"
     CDS             complement(4674..>5111)
                     /locus_tag="AC16HB_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5605431.1"
                     /translation="VPSGSINVVPGRCRFSLDIRATNDPARDACVRDTLAELARICER
                     RGLRYTLQETMRQPAAPGSPAWQQRWERAVESLGLPVFRMPSGAGHDAMKLGEVLPQA
                     MLFMRGLNAGISHNPLEAITNDDAELCVRAFQNLLEQVSSEHA"
BASE COUNT          778 a         1721 c         1791 g          821 t
ORIGIN      
        1 ggatcggccg caagaagctg ctcaatatcc tgcaggcgcg ctgcgaggcg gtcggtgtga
       61 agctggtatt cgagcgcgag gtcgatgccg acaccgagtt ccccgacgcg gacctgatcg
      121 tcgccagcga cggcatcaac tcgaagatcc gcacgatgta cgccgagaac ttccggcccg
      181 acatcgtcac gcggcccaac cgctacatct ggctcggcac gaacaagctc tacgacgcgt
      241 tcacgttcct gttcgagaag accgagcacg gctggttcca ggcgcacatc tacaggttcg
      301 acgagacgac ctcgacgttc atcgtcgaat gcccggagaa ggtctggctc gcgcatgggc
      361 tggacagggc ggaccaggcg cagtcgatcg agttctgcga gaggctcttc gcgaacaacc
      421 tgcgcggcga gaagctgatg accaacgcgc gccacttgcg cggctcggcg tggctgaatt
      481 tccagcgcgt caagtgcgag cagtggtcgc acttcaacgg tcgcagccat gtcgtgctga
      541 tgggcgacgc cgtgcacacc gcgcacttcg cgatcggctc cggcaccaag ctcgcgctcg
      601 aggacgcgat cgagctcacc gcgcagttcc agcgcctggg cgacacgcgc gaccgcatcg
      661 ccgaggtgct ggcgcgctac caggagatcc gtgcggtcga cgtgatccgg ctgcagaacg
      721 cggcgtggaa cgcgatggag tggttcgagg tctgcggcga gcgctactgc gaccagctcg
      781 agccggagca gttcatgtac tcgatgctca cgcgcagcca gcgcatcagc cacgagaacc
      841 tgcggctgcg cgacaagggc tggctcgagg gattcgagcg ctggtttgcc gagcggaacg
      901 cgagcgtcga tcctcgggca ggcatcggcg cggcgcttgc cgtgccgccg atgttcaccc
      961 cgtatacggt gcgctcggtg cggctgaaga accgcgtggt cgtctctccg atggcgcagt
     1021 actcgtgcgt cgacggcgta cccggcgaat tccacctcgt ccacctgggg gcgcgcgcgc
     1081 tgggcggtgc cggcatggtg gtggcggaga tgacctgcac ctcgcccgat gcgcgcatca
     1141 cgccaggctg cccggggctg tggaacgaga cgcagcgcga cgcgtggcag cggatcgtcg
     1201 acttcgtgca tcgcgagacc gatgcgaaga tcgcgatgca gctcggccac gccggcgcca
     1261 agggctcgac caacgcaccg tgggacggct ccggcgccga ctacccgctg ccggccgaca
     1321 actggtcgct gatctccgcc tccgcggtgc cttatctgga tgatgggccg gtgccgcgtg
     1381 cgatgacgct tgccgacatg cagcgcgtgc ggggagattt cgtgcgcgcc gcgcgccacg
     1441 cggccgaggc gggattcgac tggctggagc tgcactgcgc gcacggctat ctgctgtcgg
     1501 ctttcatctc tccgctcacc aaccggcgcc gcgacgagta cggcggcgcg ctcgccaacc
     1561 ggctgcgctt tccgctcgag gtgttccgcg cgctgcgcga ggtctggcca aagcagttgc
     1621 cgatgtcggt gcgcatttcc gcgcacgact gggtcgacgg cggcatcacg cccgacgacg
     1681 cggtcgagat cgccaaggca ttcaaggccg ccggcgccga catgatcgac tgctcgtcgg
     1741 ggcaggtcag tccgcaccag aggcctactt acggccgcat gtaccagacc ccgttcgccg
     1801 accgcatccg caacgaggcg ggcatcgcga cgatcgcggt gggcgcgatc ttcgaggccg
     1861 atcacgtcaa cagcatcgtc gctgccggac gcgcggatct gtgcgcgatc gcgcggccgc
     1921 acctcgccaa ccccgcatgg acgctgaccg aggcagcgaa gatcggctac accgcgattg
     1981 cgtggccgaa acagtatcgg caggccaagc agcagatgga gagcaatttc gcgcgcgaca
     2041 aggcggcgcg cgccgcgtaa gccgatgcgc aagcggctgc tgctgcgcca cgccgacgtg
     2101 ctcgcgacga tgaacgacgc gggcgacgag atccgcgatg gcgcgctgct catcgacgac
     2161 cagcgcatcg tctgggtcgg tgcgagcgat gacctcgatc cgcgactcgc ggagggcgcc
     2221 gaagcgatcg acacgcgcgg ctgcgtcgtc gtgccggggc tggtcaactg ccatcaccac
     2281 ctgttccaga cgctgacgcg cgcgatcggc accgggcgcg ggctgcggct cttcgactgg
     2341 ctgaagttcc tgtatccgat ctgggctgag ctgacgcccg aggccgtgta cgttagcgcc
     2401 aaggtcgggc tgatcgagct ggtgcagtcc ggctgcacga cggtcgcgga tcacctgtac
     2461 ctgtatccga acggcacgcg catcgacgac gagatccgcg cggcagccga agtcggcgtg
     2521 cggttccatc cgacgcgcgg ctcgatgagc ctcggtgcga gccgcggcgg actgccgccc
     2581 gatcgcatct gcgaggccga ggacgcgatc ctcgccgaca gcgtgcgcgc gatcgaggtt
     2641 ttccacgatc cggcgccgct gtcgatgctg cgcgtggggc tggcgccgtg ttcgccgttt
     2701 tcggtgacgg cggcgctgat gcgcgagtcg gcgcgcctgg cgcgagggca tccgcgcgtg
     2761 cacctgcaca cgcatctcgc cgagacgcgg gacgaggatc gctactgcct gcagatgttc
     2821 ggcaagcggc cgctggagta cgccgaatcg gtggagtgga tcgggtccga cgtgtggttc
     2881 gcgcacatgg tgcatccgga cgacgcggag atcacgcggc tcgcacacac gcgctcgggc
     2941 gtgtgccact gcccgtcgtc gaacatgatc ctggcgtcag ggattgcgcc ggtgcgtgcg
     3001 atgctcgacc gcggcgtgcg tgtcggcctg ggtgtcgacg gctcggccag caacgacggc
     3061 agccacctgc tcggcgaagc gcggcaggcg ctgctgctgc agcgggtcgg ctggcccggc
     3121 ttcgagtccc gtgccgatcg cttctccgcg cgcgaggcgc ttcgtcttgc gacgcgcggc
     3181 ggtgcacagg tgctcgggcg cgaagacatc ggcgtgctcg cacccggcat agcggccgac
     3241 gtggtcgcat tccgcgtcga tgacctctcg cacgcgggcg cgcgcggcga tgcggtcgcc
     3301 gcgctcgtca cctgtgcgcc ggcgcaagcg tggctgacgg tgatcgacgg ccgcgtcgtg
     3361 gtgcgcgacg ggatctatct cggcgcggaa ctgccgccgc tcgtcgaacg ccacaatgcg
     3421 ctcgcgcgcg aaatgctcga gcgcgccggc gtccgatcag gctaacaagt caagcagcgt
     3481 gcgcgcgaag accttggtcg cgcgccgcaa atcctccagc acgaggtgtt cgtccgcgcg
     3541 cttggcgttg ctctccagca cggtgcgcgg gccggcgccg tagatcaccg ccggaacgcc
     3601 gcgctcgcca tagaggcgcg cgtccgtgta cagcggcgtg cccgaggtcg ggatcggttc
     3661 gccgaagatc gcctggccgt gcttctggat tgccgccacc agcggcgcgt tgccggcaag
     3721 cggcttcagc gcgtttgcca gcagcaggcg tttcaactcg atgcggatgc cggggcaggg
     3781 ggtgagcgtg cgctcgatcc gtccgcgcac ctcggcctcg accgccgcac tgtcctcctc
     3841 cgggatcatg cggcggtcga gcttcagcac gaccttgccc ggcaccacgt tggtattcgt
     3901 gccgccgtcg atgcggccga cgttcaggta cgggtgcgtg atgcccgcga tccgcgagcg
     3961 gcgccgccgc agcacgtcgt tgtactcata cagtgcggac aggagccggt tcgccgcctg
     4021 cagcgcgtcc acgccggttt ccggatatgc ggcatgcgag gccacgccgt gcagcgtcac
     4081 ttcgaactgc agacagccgt tgtgcgcggt gaccacctcg tagctgaaac cggcggcgat
     4141 cagcagatcg ggcttcgtca gtccctgctg cagcagccag gcggggccga gcagtccgcc
     4201 gaattcctcg tcgtaggtga agtgcagttc gaccgtgccc ttcggcctcg tacccagcgc
     4261 gtcgagcgca cgcaccgcga acgtgtaggt ggcgatatcg ctcttgctga cggcggcacc
     4321 gcggccgaac agcttgccgt cgacgatctg cgcgccgtac ggatcgtgcg tccagccctc
     4381 gccgggcggc accgcgtcgc cgtgcgcatt caacgcgatg atcgttccct cgccatagcg
     4441 gcgccgtacg atgaggttgg tgaccgaaat cagcccatgg tcccggacga gcgactccgg
     4501 caccgggtgc cgctcggctt cgaggcccat gccatgcagc agttctgcgg tgcgctcggc
     4561 gtgcggcgca ttgttgccgg gcggcgtgtc ggtcggcacg cgcaccagtt cctgaagaaa
     4621 ccggacttcc tcgtcgaagt gggcgtcgat ccacccgtcg agcgctccgt ggctcacgcg
     4681 tgctccgacg acacttgttc gaggagattc tggaaggcgc gcacacagag ttccgcatcg
     4741 tcgttggtga tcgcctccag cgggttgtga ctgatgccgg cattgagtcc ccgcatgaac
     4801 agcatcgcct gcggcaggac ttcgccgagc ttcatagcgt cgtggccggc gccgctcggc
     4861 atccggaaga ccggcaggcc gagcgattcg accgcgcgtt cccagcgctg ctgccacgcc
     4921 gggctgcccg gcgcggcggg ctggcgcatc gtctcttgga gcgtatagcg caggccgcgc
     4981 cgctcgcaga tgcgcgcgag ttcggcgagc gtgtcgcgca cgcaggcgtc acgcgcggga
     5041 tcgttcgtgg cgcggatatc gaggctgaac cggcagcggc cgggaacgac gttgatcgag
     5101 ccgctcggta c
//
LOCUS       DBNHBY010000051        10184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1558933, whole genome shotgun sequence.
ACCESSION   DBNHBY010000051 DBNHBY010000000
VERSION     DBNHBY010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1558933"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..994)
                     /locus_tag="AC16HB_01200"
     CDS             complement(8..994)
                     /locus_tag="AC16HB_01200"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR5605432.1"
                     /translation="MTARPICTRIAGTGSALPGRAVGNAELAQRLATRGVETSDEWIV
                     ARTGIRQRYLAEPERSTAELGTRAAREALQAAGCDASAVDLIIVATSTPDQIFPSTAC
                     LVQAQLGVGGAAAFDVQAVCSGFVYGVTVADALIRTGNHRRALVIGAEMFSRILDWND
                     RTTCVLFGDGAGAIVLEAAEQPGILASRLHADGSQASILCTAGRVSEGAVVGDPFLRM
                     DGQAVFRLAVNVLDASAREVLADAGMNTSQIDWLIPHQANVRIMSATARKLGVKEDRV
                     VVTVDRHGNTSAASVPLALDEAVRDGRVRRGQVVMLQGVGGGFTWGSVLLRY"
     gene            complement(991..2043)
                     /gene="plsX"
                     /locus_tag="AC16HB_01205"
     CDS             complement(991..2043)
                     /gene="plsX"
                     /locus_tag="AC16HB_01205"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR5605433.1"
                     /translation="MKVRVSIDSMGGDHGPTVTVPAAIAFARAEPDAQLLLVGRQDEI
                     EARLKDATDVRAQLEVVHAEEVVAMDEPPAQALRSKRNSSMRVAVNLVKEGRADACVS
                     AGNTGALMAIARFVLKTLEGIDRPAIASVLPNQKGGVTTVLDLGANVDCTPEQLLQFA
                     VLGSALVAAVDEKERPSVGLLNIGEEVIKGNDVVKRAAELLRAGPLNFYGNVEGDDIY
                     KGTTDVVVCDGFVGNVALKASEGLAQLLSGMVKEEFTRTPLTKLQALVAYPVLRHFRK
                     RVDPRNYNGASLLGLRGVVVKSHGSADAYSFEQAVRRGFEEARHGLPERTEAALKPLL
                     AQLGTAVRPAAAEAPS"
     gene            complement(2107..2289)
                     /gene="rpmF"
                     /locus_tag="AC16HB_01210"
     CDS             complement(2107..2289)
                     /gene="rpmF"
                     /locus_tag="AC16HB_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5605434.1"
                     /translation="MAVQQNKKSASKRGMHRAHDFLTNPPTAIEPATGEVHRRHHVSP
                     TGYYRGKKVIQTKSDE"
     gene            complement(2357..2866)
                     /locus_tag="AC16HB_01215"
     CDS             complement(2357..2866)
                     /locus_tag="AC16HB_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5605435.1"
                     /translation="MTEPVGEVAPTIDVFALARTHGQLEGRVELARLPRLAPMLASTD
                     SAIDWRLTGESDARGRPTARLALRGTLVVKCDRCGLSLELPTDSESSFWFVGTEEELN
                     AQPIEVGTAEPLLGSRHFSVAQLVEDELILAVPISPRHLGCRAPDEPELDSDRHRPLA
                     LLAALKSRH"
     gene            2891..3469
                     /locus_tag="AC16HB_01220"
     CDS             2891..3469
                     /locus_tag="AC16HB_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HIR5605436.1"
                     /translation="MQTIVLASTSRYRRELLARLRVPFEVAAPSIDEVALPGEAPEAT
                     ARRLALAKAQAIARRVPGAIVIGSDQVADVDGEPLSKPGAHEAALAQLQRMQGRTIVF
                     HTALAVVHGAQKEVDSVPTTVRFRTLPRTALEAYLHAEQPYDCAGAAKIESLGIALVE
                     SVRSDDPTALIGLPLIRLTTMLAACGVELPLR"
     gene            3466..4221
                     /locus_tag="AC16HB_01225"
     CDS             3466..4221
                     /locus_tag="AC16HB_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5605437.1"
                     /translation="MSGVLYLLPALLGNTKAEDVLPQATLEAARALDYVLAEDARSAR
                     ALLKAIGHPKPIAQIRVVEIGHEPDAAQIDDWLAPLARDCIDAAVVSEAGCPGVADPG
                     ANVVAHAHALGITVRPLVGPSSILLALMAGGLNGQQFRFVGYLPRETAPLAQRLRELE
                     RDAQRGETQIFIETPYRNERLFVAILAACKSTTRLSLAVDLTTPAERIATHTVGQWAA
                     LPTERRPPLDRHPAVFSLLAGPDRTRAKWSGRQ"
     gene            complement(4152..4559)
                     /locus_tag="AC16HB_01230"
     CDS             complement(4152..4559)
                     /locus_tag="AC16HB_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5605438.1"
                     /translation="MVDAGEVIDLGDAAALRESGAGIRFDLLAGTEPATGFVIRHRGA
                     VHGFLNRCAHVAMELDWQPGEFFDFDREFLICATHGALYEPASGACIGGPCAGHMQLL
                     RLDVFERGGRIYWRPDHFARVRSGPASSENTAG"
     gene            complement(4538..5194)
                     /locus_tag="AC16HB_01235"
     CDS             complement(4538..5194)
                     /locus_tag="AC16HB_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR5605439.1"
                     /translation="MLERFDLVVFDWDGTLIDSTATIARSIQAAAADLGLPVPDFERA
                     SHVIGLGLHDALAGAVPDLEPSRIAEFSARYRVHYLAAEDEFDLFGGARELVEALRAR
                     GALLAIATGKSRAGLARALDAARFNGVFAATRCADQTHPKPHPAMLLELTAELMVEPE
                     RTLMIGDTTHDLQMAAAARTHAVGVTHGAHPADALRNLAPLALFDSLHDLHAWLMPAK
                     "
     gene            complement(5187..6176)
                     /locus_tag="AC16HB_01240"
     CDS             complement(5187..6176)
                     /locus_tag="AC16HB_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5605440.1"
                     /translation="MDVPGKARAESGASEAVSADRVALITVGPDGAGQRLDNYLLKLA
                     RGVPKSHVYRIVRSGEVRVNKARATVSQRLAAGDVLRIPPLRTAMPSAARPAPLAELP
                     PVLFEDEHLLVVDKPAGLAAHGGSGIAFGLIERLRAARPELPLLELAHRLDRETSGVL
                     LLAKTRRALVHLHEQLREGQVDKRYLALVAGDWVNDRQHVRAPLTKYLTKSGERRVSV
                     DPAGMPSHTVFALRERLGRFSLLEAQLKTGRTHQIRVHLGHLGFPIAGDDKYGDFELN
                     RRLARGEFGPKFGRMFLHAWRTAFAHPVGGVPLALEAPLPADCDHLLRQLRNA"
     gene            6721..9453
                     /locus_tag="AC16HB_01245"
     CDS             6721..9453
                     /locus_tag="AC16HB_01245"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR5605441.1"
                     /translation="MKRMLFNATHAEELRVAIVDGQKLIDIDIETAGREQRKSNIYKG
                     VVTRVEPSLEACFVDYGEERHGFLPFKEISRSYFKPGVDVGRARINEAIAEGQEMVVQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRQELREAVDKLELP
                     SGMSVIARTAGIGRAVEELQWDLNYLLQLWHAIEDASTPLYRDAASATVKEATTDGQK
                     NKRLNPAPFLIFEESSLVIRAIRDYFQPQIGEILVDTDDIAEQARQFMAHVMPDMVNR
                     VKRYKDDIPLFSRFQIEHQIETAHSRTVPLPSGGAIVIDHTEALVSIDVNSARATKGA
                     DIEETALRTNLEAADEAARQLRLRDLGGLIVIDFIDMEDAKNQRAVENRLKDALHYDR
                     ARVQMGKISRFGLMELSRQRLRPALSEGSHVTCPRCNGVGVIRDTDSSALHILRILQE
                     EAMKESTAAIHAQVPVDVATYLLNEKRTDIAKLEARHRISVVLIPNKYLETPHYKVER
                     LKHDDERLDQAKASYDRAEAPSFETVYGAPKAGEGERARPKQEAVVRGIVPETPAPVV
                     VPPPEPAPAPAAAPMVVAAAPAPSLWSRIKRMFTGEPEVVAAPVAAAALRPTVPPRAE
                     RPTAEPGRERGERGERGRDRGRRDERRGRDAQRGPRPEGQKRDQRPQRQDRGEERDRK
                     PAEARPEGESRERRPPRAERGPRPQAEAPRAEAIEVRAPIADSAAPVTAPLAAPVAAI
                     EAPMVPGAAEGEGDERRGRRRRRRRGRGGERGEERADAAMAQADADEVSPETAAPLAR
                     EEEAPAPITPIAAEPVVAATPMPEPQPAAQIVLPRAEAKPMAVESLTPLLEKAGLILV
                     QTAPDKHAEAQQRIAAEPRPLRVPRERPALPPLDVGPLIQVETRRRDSQPSPSA"
     gene            complement(9470..9922)
                     /locus_tag="AC16HB_01250"
     CDS             complement(9470..9922)
                     /locus_tag="AC16HB_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5605442.1"
                     /translation="MKWVTRERPKIDRIACPWLIARFIDREAEFLFVPSAQVQEVARS
                     VSAIPFDVPGVELTHVGDLCSFDAFLRKYALSQPGLETLALIVRAADTDRLDLAPAAA
                     GLLAISLGLGELYEDDQALLAQGMVVYDALYAWCRSARTERHTWNYRS"
     gene            complement(9919..>10184)
                     /locus_tag="AC16HB_01255"
     CDS             complement(9919..>10184)
                     /locus_tag="AC16HB_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5605443.1"
                     /translation="VRRRPVFDGAEHRIENARWFDPEQVDGWACDLDRARPVALYCVH
                     GHAISRSTALALKRRGYDAYCVAGGIEAWQARALPLEAKGGER"
BASE COUNT         1715 a         3584 c         3391 g         1494 t
ORIGIN      
        1 catcttgtca gtaacgaagc agtaccgatc cccacgtgaa tccgccgccc acgccctgca
       61 gcatcacgac ctgcccccgc cgtacgcggc cgtctcgcac cgcttcgtcg agcgccagcg
      121 gcaccgaggc ggccgacgtg ttgccgtggc ggtcgacggt tactacaacg cggtcttcct
      181 tcaccccgag cttgcgtgcc gtggccgaca tgatgcgcac gttcgcctga tgcgggatca
      241 gccagtcgat ctgcgacgtg ttcatgccgg cgtccgcgag cacctcgcgc gccgatgcat
      301 cgaggacatt gaccgccagc ctgaacacag cttggccgtc catgcgcaga aacggatcgc
      361 cgacgactgc gccttcggac acgcgcccgg ccgtgcacag gatcgatgcc tgcgatccgt
      421 ccgcatgcag gcggctggcc aggatgccgg gctgctccgc cgcctccagc acgatcgcgc
      481 cggcgccgtc cccgaacaat acgcaggtcg tgcggtcgtt ccagtcgagg atgcgcgaga
      541 acatctcggc gccgatcacc agcgcgcgac gatgattgcc ggtccggatc agcgcatcgg
      601 cgaccgtcac accgtagacg aacccgctgc agacggcctg cacgtcgaat gccgccgcgc
      661 cgccgacccc gagctgtgcc tggaccaggc atgcggtgga cgggaagatc tggtctggcg
      721 tcgacgtcgc cacgatgatc aagtcgaccg cgctcgcatc gcacccggca gcctgcaggg
      781 cctcgcgtgc cgcgcgggtg ccgagctcgg cggtcgaccg ttcgggctcc gccagatagc
      841 gctgccgaat gccggtgcgc gcgacgatcc attcatccga cgtttcgacg ccacgggtgg
      901 caagccgctg cgccagctcg gcgttgccga ccgcgcggcc gggcaatgcg cttccggtgc
      961 ccgcgatgcg cgtgcagatc ggacgcgcgg tcacgacggc gcctccgcgg cggccggtcg
     1021 caccgccgtg cccagctgtg cgagcaacgg cttcagcgcc gcttcggtgc gttcgggcag
     1081 accgtgccgc gcttcctcga atccgcgccg caccgcctgc tcgaaggagt aagcatccgc
     1141 cgagccgtga ctcttgacca cgacgccgcg caggccgagc aggctggcgc cgttgtagtt
     1201 gcgcggatca acgcgcttgc ggaaatgtcg cagcaccgga taggcaacga gagcctgcag
     1261 tttcgtcaac ggcgtgcggg tgaattcttc cttcaccatc ccgctgagca gttgtgccag
     1321 tccctcggac gccttcagcg cgacgttgcc gacgaagccg tcgcaaacca cgacgtccgt
     1381 cgtgcccttg tagatgtcgt cgccctcgac attgccatag aagttcagag gacccgcgcg
     1441 cagcagctcc gccgcgcgct tgaccacgtc gttgcccttg atcacttcct caccgatatt
     1501 gagcaggccc acgctgggcc gctccttctc gtcgaccgcc gcaacgagcg cggagccgag
     1561 caccgcgaac tgcagcagct gttcgggcgt gcagtccacg ttggcgccca gatccagcac
     1621 ggtcgtgacg ccgcccttct gattgggcag cacggaagcg atcgccggcc ggtcgatgcc
     1681 ctccagcgtc ttcagtacga agcgcgcgat tgccatcagc gcaccggtgt tgccggcact
     1741 gacgcaggcg tccgctcgcc cttctttgac gagattgacc gcgacccgca tcgacgaatt
     1801 gcgcttgctg cgcagcgcct gcgcgggcgg ctcgtccatc gcgaccacct cctccgcgtg
     1861 cacgacctcg agctgggcgc gcacgtcggt tgcgtccttc aggcgcgctt cgatctcgtc
     1921 ctgccggccg accagaagca gttgcgcgtc cggctccgca cgcgcgaacg caatcgctgc
     1981 cgggaccgtc accgtcgggc cgtgatctcc gcccatggag tcgatggaaa cccggacctt
     2041 catcgtcccg acgatgacag atgcgcgtgg cgagcgtcgc ggcgcacggc cgcgccgacg
     2101 agtggcttac tcgtcgctct tggtctggat gaccttcttg ccgcggtagt agccggtcgg
     2161 gctgacgtga tggcggcgat gaacttcgcc ggtggcgggc tcgatcgcag tcggcgggtt
     2221 cgtcaggaaa tcgtgggcgc ggtgcatgcc tcgcttcgac gccgacttct tgttctgctg
     2281 gactgccatg gcattcctcg tgtgcaagct agccgcgcag tttagcgcag cgcgtcggtc
     2341 cgaagcaaaa agtacattag tggcgagact ttagcgctgc aagcagcgcg agcggacggt
     2401 gtcggtcgga atccaactcc ggctcgtccg gcgcgcggca gccaaggtgg cgcggcgaaa
     2461 tcggcaccgc caggatcaac tcgtcttcca ccagctgagc gaccgaaaag tgccggctgc
     2521 cgagaagcgg ctccgcggtg ccgacctcga tcggctgcgc gttcagttcc tcctcggttc
     2581 ccacgaacca gaacgacgac tcggagtcgg tcggcagctc gaggctcagt ccgcagcggt
     2641 cgcacttgac caccagcgtg ccacgcagcg cgagccgggc cgtcggccgg ccgcgcgcat
     2701 cggattcgcc cgtgagccgc cagtcgatcg cgctatcggt cgacgccagc atcggcgcca
     2761 ggcgcggcag tcgcgccaat tcgacacgtc cttcgagctg tccgtgcgtg cgcgccagcg
     2821 cgaagacatc gatcgtcggc gccacctccc cgaccggctc ggtcatctgc aactccaata
     2881 gactgcgtcg atgcaaacga tcgtactcgc ttcgacctcg cgctaccggc gtgaactgct
     2941 cgcgcggctg cgcgttccgt tcgaggtcgc ggcgcccagc atcgatgaag tcgcgctgcc
     3001 gggcgaggca ccggaggcaa ccgcgcgccg gctcgcgctg gccaaggcgc aggcgatcgc
     3061 caggcgcgtt cccggcgcaa tcgtgatcgg gtcggaccag gtcgccgacg tcgacggcga
     3121 gccgctgtcg aagccaggcg ctcacgaggc ggcgctcgcg caattgcagc gcatgcaggg
     3181 acgcacgatc gtgttccaca ccgcgctcgc ggtcgtgcac ggtgcgcaga aggaggtcga
     3241 ctcagttccg acgacggtgc gcttccgcac gctgccgcgc accgcgctgg aggcgtacct
     3301 gcacgccgag cagccgtacg actgcgccgg cgcggccaag atcgagtcgc tcggtatcgc
     3361 gctggtcgag tcggtccgct ccgacgatcc gaccgcgctg atcggcctgc cgctgatccg
     3421 gctcacgacg atgctcgcgg cctgcggcgt cgaactgccg ctgcgatgag cggagtgctc
     3481 tacctgctgc cggccctgct cggcaacacg aaggccgaag acgtgctgcc gcaggcgaca
     3541 ctcgaggccg cgcgcgcact cgattacgtg ctggccgaag acgcgcgcag cgctcgcgcg
     3601 ctgctgaaag cgatcggcca tccgaaaccg atcgcgcaga tccgggtcgt cgagatcggc
     3661 cacgaacccg atgccgcgca gatcgacgac tggctggcgc cgctcgcgcg cgactgcatc
     3721 gacgcggctg tcgtatcgga ggccggctgc cccggcgtcg cagatccggg cgcgaacgtg
     3781 gttgcgcatg cgcacgccct cgggatcacg gtacgtccgc tcgtcggtcc ctcttcgatc
     3841 ctgctcgcgc tgatggctgg cggactgaac ggccagcagt tccggttcgt cggctacctg
     3901 ccgcgcgaga ccgcgccgct cgcgcaacgg ctgcgcgagc tcgagcgcga tgcacagcgc
     3961 ggcgagacgc agatcttcat cgagacgccc taccgcaacg agcggctgtt cgtggcaatc
     4021 cttgctgcct gcaagtcgac gacccggctg agcctggcgg tcgacctgac gacgcctgcc
     4081 gaacgcatcg cgacacacac ggtcgggcaa tgggcagcgc tgccgactga gcgtcgtccg
     4141 ccgctcgacc gtcacccggc ggtgttctcg ctgctcgcgg ggcccgatcg cacgcgcgcg
     4201 aagtggtccg gccgccagta gatccgtccg ccgcgctcga agacatcgag ccgcagcagt
     4261 tgcatgtggc ccgcgcacgg acccccgatg cacgcgccgg aggcgggctc gtacagcgcg
     4321 ccgtgggtcg cgcagatcag gaactcgcgg tcgaagtcga agaactcacc cggctgccaa
     4381 tcgagttcca tcgccacgtg cgcgcagcgg ttcagaaagc cgtgtaccgc gccgcgatgg
     4441 cggatcacga atcccgtcgc cggttcggtg ccggccagaa ggtcgaagcg aatgccggcg
     4501 ccgctctcgc gcagcgccgc cgcatcgccg aggtcgatca cttcgccggc atcaaccatg
     4561 cgtgcaggtc gtgcagggaa tcgaagagcg ccagcggcgc cagattgcgc agagcgtcgg
     4621 ccggatgggc gccgtgcgtg acgccgaccg cgtgcgttcg tgcggccgcc gccatctgca
     4681 ggtcgtgggt cgtgtcgccg atcatcaacg tgcgctccgg ctcgaccatc agttccgcgg
     4741 tcagttcgag caacatcgcc gggtgtggct tcggatgcgt ctggtcggcg cagcgcgtgg
     4801 cggcgaacac accgttgaag cgcgccgcat cgagcgcgcg tgcgagcccg gcgcggctct
     4861 ttccggtggc gatcgcgagg agcgcgccgc gcgcacgcag tgcctcgacc agttcgcgcg
     4921 cgccgccgaa gaggtcgaac tcgtcctcgg ccgccaggta atgcacgcga tagcgcgccg
     4981 agaactccgc gatacgggat ggctcgagat caggcaccgc gcctgccagc gcatcatgca
     5041 gtcccagccc gatcacgtgg ctggcgcgct cgaagtcggg gacaggaagt cccaggtcgg
     5101 ccgcggctgc ctggatgctg cgcgcgatcg tcgcggtcga atcgatcagg gtgccgtccc
     5161 agtcgaacac gaccaggtcg aatcgttcaa gcattgcgca attgtcgcag caggtgatcg
     5221 cagtcggctg gcagcggcgc ttcgagcgcc agcggcacac cgccgaccgg gtgcgcgaat
     5281 gcggtgcgcc acgcgtgcag gaacatcctc ccgaacttcg ggccgaactc accgcgcgcc
     5341 agccgccggt tcaattcgaa atcgccgtac ttgtcatcgc ccgcgatcgg gaagcccagg
     5401 tgcccgagat ggacccggat ctggtgcgtg cggccggtct tcagctgcgc ctcgagcaac
     5461 gagaagcggc cgagccgctc gcgcagcgca aagactgtgt gcgatggcat acccgctgga
     5521 tcgaccgata cgcgccgctc gccgctcttc gtcaagtact tcgtcagcgg cgcgcggaca
     5581 tgctggcgat cgttgaccca atcgccggcc accagtgcga gatatcgctt gtcgacctgc
     5641 ccttcgcgca attgctcgtg caggtgcacc agcgcacgcc gcgtcttggc aagcagcagc
     5701 acgcccgacg tctcccggtc cagccgatgc gccagttcca ggagcggaag ctcgggacgc
     5761 gccgcgcgga gccgctcgat cagcccgaac gcgatgccgc tgccaccgtg ggctgccagc
     5821 ccggccggct tgtcgaccac cagcaggtgc tcgtcctcga acagtaccgg cggcaattcg
     5881 gcgagcggcg ctgggcgcgc cgccgatggc atcgccgtgc gcagcggcgg aatccgcagc
     5941 acgtcgccgg ccgccagccg ctggctcacg gtggcgcgcg ccttgttcac ccgcacctcg
     6001 ccgctgcgca cgatgcggta gacatgactc ttcggtacgc cgcgggccag cttcagcaga
     6061 tagttgtcga gccgctgacc ggccccgtcg gggccgaccg tgatcagcgc gacccggtca
     6121 gccgaaacgg cctccgacgc gccgctttca gcgcgcgctt tgcccggaac gtccatgttg
     6181 catatacttg tgcgcgcgtt gaaatcaggc agcgcacttc gagtgatggc tcaggaatcg
     6241 gccccgcccg gcggggcatc gcagtcgagc cagttgccgc gaattcgacg cgagtcgatt
     6301 ctagcgatcg aacgcccccg gaccggatgc ggaatcgcag aatcgacgat ggcgaaccgg
     6361 ccgagagttc ggaccggaaa gtgaacagcg cggcgacgac gcggttgccg cagtgagtaa
     6421 cccgagggca gtgcgtgacg gtcatgggac caaccgccgc cccgctgaat cgaatcgagc
     6481 ttggcccgtc gctgtgggcg ggcgtgacgc aaccgactgc atgatcagca cacgagccaa
     6541 caccgtcctg gctgcaagcg ccagcaacgg cacggaaccg gcatggccgg cagaatctct
     6601 gccgcacggc ccgccgggga accgactcgc ctgacccgct gtccgccgct gcgcgcggcg
     6661 cgttcctgcc cgattcgatc cagcactgaa gcgccccgcg aagaggcgca ggagagctga
     6721 atgaaacgga tgctattcaa tgccacgcac gccgaagaac tgcgtgtggc gatcgtcgac
     6781 ggccagaagc tgatcgacat cgacatcgag accgccggcc gcgaacagcg caagtcgaac
     6841 atctacaaag gggtggtcac ccgggtcgag ccgagcctgg aggcctgctt cgtcgactac
     6901 ggcgaggagc gccacggctt tctgcccttc aaggagatct cgcgcagcta cttcaagccc
     6961 ggggtcgacg tcggacgcgc acgcatcaac gaggcgatcg ccgaaggcca ggagatggtc
     7021 gtgcaggtcg agaaggagga gcgcggcaac aagggcgccg cgctgaccac tttcatctct
     7081 ctcgcgggcc ggtatctcgt gctgatgccg aacaatccgc gtggcggcgg cgtgtcgcgc
     7141 cgcgtcgagg gggaggaccg gcaggaactg cgcgaagcgg tcgacaagct cgaactgccg
     7201 tccggcatga gcgtgattgc gcgcaccgcc ggcatcggcc gcgcggtcga ggaactgcag
     7261 tgggacctca actacctgct gcagctgtgg catgcgatcg aggatgcatc cacccctctg
     7321 taccgcgacg cggccagcgc aacggtcaag gaagcgacga ccgacggcca gaagaacaaa
     7381 cggttgaatc cggcgccctt cctgatcttc gaggaatcga gcctcgtgat ccgtgcgatc
     7441 cgcgactact tccagccgca gattggcgag atcctggtcg acaccgacga catcgccgag
     7501 caggcgcgcc agttcatggc gcacgtgatg ccggacatgg tcaaccgggt gaagcgctac
     7561 aaggacgaca ttccgctctt ctcgcgcttc cagatcgagc atcagatcga aaccgcgcac
     7621 tcgcgcacgg taccgctgcc gtcgggcggc gcgatcgtga tcgaccacac cgaggcgctg
     7681 gtgtcgatcg acgtcaactc ggcacgcgcg accaagggcg ccgacatcga ggaaacggct
     7741 ctgaggacga atctggaagc cgccgacgag gccgcgcgcc agctgcgcct gcgcgacctt
     7801 ggcggtctga tcgtcatcga cttcatcgac atggaggacg cgaagaacca gcgcgcagtc
     7861 gagaaccgcc tgaaggacgc gctgcactat gaccgcgcgc gggtgcagat gggcaagatc
     7921 tcgcgcttcg ggctgatgga actgtcgcgc cagcggctgc ggccggcgct ctcggaaggc
     7981 agccacgtca cctgcccgcg ctgcaatggc gtcggtgtga ttcgtgacac cgacagttcg
     8041 gcactgcaca tcctgcgcat cctgcaggaa gaggcgatga aggaaagcac cgccgcgatc
     8101 cacgcacagg tgccggtcga cgttgccacc tacctgttga acgagaagcg gaccgacatc
     8161 gccaaactcg aggcacgcca tcgcatctcg gtcgtgctga tcccgaacaa gtatctggag
     8221 acgccacact acaaggtcga gcgactgaag cacgacgacg aacggctcga ccaggccaag
     8281 gccagctacg accgcgccga ggccccctcg ttcgaaaccg tttacggtgc gccgaaggca
     8341 ggcgaaggag agcgcgcgcg gccgaaacag gaggccgtcg tgcgcggaat cgttcccgaa
     8401 accccggcgc cggtcgtggt gccgccgccc gaacccgcgc cggcacccgc tgcggctccc
     8461 atggtcgttg ccgccgcgcc ggcgccgtcg ctttggagcc gcatcaagcg gatgtttacc
     8521 ggcgagccgg aagtcgtcgc cgcaccggtg gcggcggctg ctctgcgtcc gaccgtgcct
     8581 ccacgcgccg agcgccccac cgcggagccc ggtcgcgaac gcggcgagcg cggcgaacgc
     8641 ggacgagacc gcggccgacg cgacgagcgt cgaggccggg acgcacaacg cggaccgcgg
     8701 cccgaaggac agaaacgcga tcagcgtcca cagcgtcagg accgcgggga ggaacgcgat
     8761 cgcaagcccg ccgaggcgcg ccctgaaggt gaaagtcgcg aacgtcgtcc gccgcgcgcc
     8821 gagcgtggac cgcggccgca ggccgaagcg ccgcgcgccg aagccattga agtgcgggca
     8881 ccgatcgcag acagcgctgc accggtgacg gcacctctgg ctgcgccggt cgctgcgatc
     8941 gaggcaccca tggtcccggg tgcagccgaa ggcgaaggcg acgagcgtcg tggccggcgc
     9001 cgccgccgcc gccgcggccg cggtggcgaa cgcggcgagg agcgcgccga cgcggccatg
     9061 gcccaggccg acgcggacga ggtatccccg gagaccgccg cgccgctggc gcgcgaggaa
     9121 gaagcgccgg ctccgattac gccgatcgcc gcagagccag ttgtcgccgc gacgcccatg
     9181 ccggaaccac aaccggcggc gcagatcgtg ttgccgcgcg cagaggctaa gcccatggcc
     9241 gtcgaatcgc tgacgccact gctcgagaag gctgggctga tcctggtaca gaccgcaccc
     9301 gacaagcacg cagaagcgca gcaacggatc gcggccgaac cgagaccgct ccgcgtgccc
     9361 cgcgagcgcc cggctctgcc gccattggat gtcggtccgc tgattcaggt cgagacgcgt
     9421 cggcgcgatt cgcagccgtc gccatcggcc tgatggcacg cgcggcgcgt caactccggt
     9481 agttccacgt gtgccgctcg gttcgcgccg agcggcacca cgcatacagc gcgtcgtaga
     9541 cgaccatgcc ctgcgccagc agcgcttgat catcctcgta gagctcaccg agaccgagag
     9601 atatcgccag caaccccgcc gccgccggcg cgaggtcgag gcggtcagtg tcagcggccc
     9661 gcacgatcag cgcgagcgtc tccagccccg gctgggatag cgcgtacttg cgcaggaacg
     9721 cgtcgaagct gcacaggtcg ccaacgtggg tcagttcgac ccccggcacg tcgaagggga
     9781 tcgcggaaac gctgcgtgca acctcctgca cctgcgcgct cggcacgaag aggaattcgg
     9841 cttcacggtc gatgaagcgc gcgatcagcc acgggcaagc aatgcgatcg atcttcggcc
     9901 gttcccgcgt cacccatttc atcgttcacc ccctttcgct tccagcggca gtgcacgcgc
     9961 ctgccatgcc tcgattccac cggccacgca gtacgcgtcg tagccgcgcc gcttcaacgc
    10021 gagtgccgtg ctgcggctga ttgcgtggcc gtgcacgcag tacagtgcca ccggccgcgc
    10081 tcgatcgaga tcgcacgccc agccgtcgac ctgctccgga tcgaaccagc gcgcgttctc
    10141 gatccgatgc tcggcgccgt cgaacaccgg ccggcgccgc acgt
//
LOCUS       DBNHBY010000052         6696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_408521, whole genome shotgun sequence.
ACCESSION   DBNHBY010000052 DBNHBY010000000
VERSION     DBNHBY010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_408521"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="AC16HB_01260"
     CDS             complement(<1..899)
                     /locus_tag="AC16HB_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR5605444.1"
                     /translation="MRACAVLFAILVLLAPASAQQFPARPVTIIVPFAGGGPTDTVAR
                     LVAAGMAQDLKVPVNVENVGGSGGTVGAARVAKAAPDGHTLLLDHIGHAAAPALYAKL
                     PYDPIADFAPIGRVADVPMTLVARGNMPAKDFKQLAAYIRANGAGVNYAHAGVGAASQ
                     LCGLLLMKALGVKLTAVAYKGTAPALTDLLSGQIDLMCDQTTNTLTQIRAGRIKAYAV
                     ASPHRLPNLKDVPTFAEEGLKSVEISVWHGLYAPKGTSQPVIDRLVAALQAALKDPAA
                     VERFAEFGAQPARPADATPAALQT"
     gene            complement(1056..2258)
                     /gene="pcaF"
                     /locus_tag="AC16HB_01265"
     CDS             complement(1056..2258)
                     /gene="pcaF"
                     /locus_tag="AC16HB_01265"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR5605445.1"
                     /translation="MEQVLICDAIRTPIGRYAGSLSPVRTDDLAAIPIRALMERSPKV
                     DWAAVDDVIYGCANQAGEDNRNVGRMALLLAGLPPEVPGGTVNRLCGSSLDAVAIAAR
                     TIKSGEAALVIAGGVESMSRAPFVMPKADSAFARSNAVYDTTIGWRFVNPMMKAKYGV
                     DSMPETAENVAEQFRVARADQAAFALRSQQRALAAIASGRMAQAIVPVTIPQRKGEPI
                     VVNRDEHPRETTLEALAKLKGVVKPDGTVTAGNASGVNDGACALLLANETAARRHGLT
                     PKARVIAAAVAGVAPRIMGFGPAPATRKVLERAGLKLAQMDVIELNEAFAAQALAVTR
                     DLGLPDDAAHVNPNGGAIALGHPLGASGARLVTTATYQLARSGGRYALATMCIGVGQG
                     IALIIERV"
     gene            complement(2274..2846)
                     /locus_tag="AC16HB_01270"
     CDS             complement(2274..2846)
                     /locus_tag="AC16HB_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5605446.1"
                     /translation="MELKGEQVLTADRMTAWTALNDIEMLKMCVPGCEGFAALGENRY
                     EVVMNAAIGPVKSRFKGAVELVDIDAPNGYTMKFDSSGGAAGFARGDVRVGLADEGAG
                     TRLSYTANVQVGGKLAQVGSRLVDAAAGAMADKFFEAFDARLRARARAELEATQLRVD
                     ADLSKALPPPATFSFWSLLKAFFRRLFTRR"
     gene            complement(2837..4066)
                     /locus_tag="AC16HB_01275"
     CDS             complement(2837..4066)
                     /locus_tag="AC16HB_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5605447.1"
                     /translation="MLPVAALEQLPGRLAENVVHFARVLRSAGIPVGTDRVLLALRAL
                     RVAGIESRQDFHATLAACLIDRAEHRVLFDQAFYIFWKDPDLLGQMLRLLLPEVGQAS
                     GAPPQPENRRLGEALFKESPQARQQARAEERIEVDAHLTWSERELLRKADFDTMTTAE
                     WNAARKMLHAMRPFFAHLITRRYAPSPRGTRIDLQRLLRDAARHGGDIAALPRRTRRT
                     RPEPLVVIVDISGSMSRYSRMFLHFLHALVGIGSGSEHRTQAFVFGTRLTPITRQLAS
                     RDPDVAVAAVVQAVDDWSGGTRIAHAIHDFNLHWARRVLSGSPTVLLVTDGLEHGDTE
                     LLAREMERLSKSCRRLVWLNPLLRYEAFEPKARGIRAMLPHVDEFLPVHNIDSLHELA
                     RTLSRTTPRTGKQEPWN"
     gene            complement(4078..5388)
                     /locus_tag="AC16HB_01280"
     CDS             complement(4078..5388)
                     /locus_tag="AC16HB_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase domain-containing
                     protein"
                     /protein_id="HIR5605448.1"
                     /translation="MKRATAARAHTVREAIGRCRKLIRERGEVSSAAAVRAALASYLA
                     LDEDARERFFDALAREFSPSPSAVLEAARSYDVEPSPANLLRLQEIVEPVRQELFRRL
                     NMAPGGTAALVQMRKRVLRGLQRHPRWRVIDDDLRHLLRSWFNRGFLTLERIDWRTPA
                     VVLEKLIEYEAVHQIQGWDDLRRRLAADRRCFAYFHPQLAHEPLIFIEVALTRGISAR
                     VQPLLDIHSEVTPPAKANCAVFYSVTNCQEGLRGISFGSFLIKQVATELRAELPNIRR
                     FATLSPIPRFRGWLQSQQERLCSGAQGEAWRALFERLDAPEWPRSEAAAGAEALLLPA
                     AAYYLAQLRRGDGPYDSVAHFHLGNGAVLERLNWMGDTSPQGIERSFGIMSNYVYRLN
                     EVERNHELFVNEHRLLVSASIAQLAARAAPWLGEAARPRRDAVT"
     gene            complement(5385..6053)
                     /locus_tag="AC16HB_01285"
     CDS             complement(5385..6053)
                     /locus_tag="AC16HB_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5605449.1"
                     /translation="MFIGHYGIALAAKKFAPGTSLGTLVLAAVWIDLVWPTLLLLGLE
                     QARIDPSLGGVTPIAFTDYPWTHSLLAVGVWALLLGGGHNLWQRDRRAALVIAVLVAS
                     HWALDAIVHRPDLLLMPGGHLAIGFGLWRSPIATLIVELGTLAFGVLLYLRATTGAPR
                     GALWTLLALLVAIYFGNLFGPPPPSVQAIAWVGQAQWLIVLAAYWIDRPGMLAVPTAG
                     QEAR"
     gene            complement(6066..>6696)
                     /locus_tag="AC16HB_01290"
     CDS             complement(6066..>6696)
                     /locus_tag="AC16HB_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5605450.1"
                     /translation="IRLAEATHEAREQVATGLFTGRFLIERPLLRALLPHAVPPVLLI
                     DELDRTDEPFEAYLLEVLSDFQITIPEWRTVRAEAPPVVVITSNRTREIHDAVKRRCI
                     YHWVGYPDAARELQIVHRKVPDAKEALSRQIVAFVQSLRQIDLYKLPGVSETIEWSRA
                     LLALDAVVLEPETVDNTLGVLLKYQDDIARMQGTEAARLVEKVRAEIAA"
BASE COUNT         1023 a         2413 c         2256 g         1004 t
ORIGIN      
        1 gtctgcaatg cggcgggcgt ggcgtcggcg ggtcttgcgg gctgcgcgcc gaactcggcg
       61 aaacgttcga ccgcggccgg atccttcagc gccgcctgca gcgcggccac cagccggtcg
      121 atcaccggtt gcgacgtacc cttcggcgcg tacagcccgt gccacaccga gatctcgacg
      181 ctcttcagcc cttcctcggc gaaggtcggc acgtccttca ggttcggcag ccggtggggc
      241 gacgcgactg cgtacgcctt gatgcgtccg gcgcggatct gtgtcagcgt attggtggtc
      301 tggtcgcaca tcagatcgat ctgccccgac agcagatcgg tcagcgccgg cgcggtgccc
      361 ttgtaggcga ccgcggtcag cttcacgccg agcgctttca tcagcagcag cccgcacagc
      421 tgcgacgccg cgccgacgcc ggcgtgcgcg tagttgacgc cggcgccgtt ggcgcggatg
      481 tacgccgcca gctgcttgaa gtccttcgcc ggcatgttgc cgcgcgcgac cagtgtcatc
      541 ggcacatcgg ccacgcggcc gatcggtgcg aaatcggcga tcgggtcgta cggcagcttc
      601 gcgtacagcg ccggagccgc cgcatgcccg atgtggtcga gcagcagcgt atggccgtcg
      661 ggcgcggcct tcgcgacacg cgccgcgccg accgtgccgc cgctgccgcc gacgttctcg
      721 acgttgaccg ggactttcag atcctgcgcc atccccgccg cgaccaggcg cgcgacggtg
      781 tcggtcggcc cgccgcccgc gaacggcacg atgatcgtga ccggccgcgc agggaactgc
      841 tgcgcgctcg ccggcgccag caggacaagg atcgcgaaca gaacggcaca ggcacgcatc
      901 ggatgcctcc gaagcaggac atcctcactg ctgcacgacg gacatgacga actacgttac
      961 gcccgctccc ggcgccctgc caagcacgtc ggaaggtgac acggtcgacg cgcggcgcgt
     1021 ggcggccgcg gaccgattcg agcccgcagg cgtcgtcaca cgcgctcgat gatcagcgcg
     1081 atgccctgcc ccacgccgat gcacatcgtg gcgagcgcgt agcggccgcc gctgcgggca
     1141 agctggtagg tcgcggtcgt caccagccgc gcgccactgg cgccgagcgg atggccgagc
     1201 gcgatggcgc caccgttcgg gttcacgtgg gcggcgtcgt cgggcagccc cagatcgcgc
     1261 gtgaccgcga gcgcctgcgc ggcgaacgcc tcgttgagct cgatcacgtc catctgcgcc
     1321 agcttcagcc ccgccctctc gagcaccttg cgcgtcgccg gcgcggggcc gaagcccatg
     1381 atccgcggcg ccacgcccgc cactgccgcc gctatcacgc gcgccttcgg cgtcaggccg
     1441 tggcggcgtg cggccgtttc gtttgccagc agcagcgcgc acgcgccatc gttcacgccc
     1501 gacgcattgc ccgccgtgac cgtgccgtca ggtttgacca cgcccttgag cttcgccagc
     1561 gcctcgagcg tcgtctcgcg cggatgctcg tcgcgattga cgacgatcgg ctcgcccttc
     1621 ctctgcggga tcgtcaccgg cacgatcgcc tgcgccatgc gcccggacgc gatcgctgcc
     1681 aacgcgcgtt gctggctgcg cagcgcgaac gcggcctggt cagctctcgc gacccggaac
     1741 tgctcggcca cgttctcggc ggtctccggc atcgagtcga cgccgtactt cgccttcatc
     1801 atcgggttga cgaagcgcca gccgatcgtc gtgtcgtaca cggcattgct gcgcgcaaac
     1861 gcgctgtcgg ccttcggcat cacgaagggc gcgcgcgaca tgctctccac gccgccggcg
     1921 atcaccagtg ccgcttcgcc gctcttgatc gtgcgcgccg cgatcgccac cgcgtccagg
     1981 ctcgagccgc acagccggtt gaccgtcccg cccggcactt ccggcggcag tcccgccagc
     2041 agcagcgcca tgcggccgac gttgcggttg tcctcgcccg cctggttggc gcagccgtag
     2101 atcacgtcgt cgactgcggc ccagtcgacc ttcggactgc gctccatcag cgccctgatg
     2161 ggaatggcgg cgaggtcgtc ggtgcgcacc ggcgacaggc tgccggcata gcggccgatc
     2221 ggagtgcgga tcgcatcgca gatcaatacc tgttccattc gcatgctcct cgtctatcgc
     2281 cgcgtgaaca gccgcctgaa gaaagccttc agcaggctcc agaagctgaa tgtcgccggc
     2341 ggcggcagcg ccttcgacag gtcggcatcg acacgcaatt gcgtcgcttc gagttccgcc
     2401 cgggcacgcg cgcgcagccg cgcgtcgaat gcctcgaaga acttgtcggc catcgcgccc
     2461 gccgcggcgt cgaccaggcg cgagccgacc tgtgccagct tgccaccgac ctgcacgttg
     2521 gcggtgtagg acaggcgcgt gccggcgccc tcgtcggcca gccctacgcg cacgtcgccg
     2581 cgtgcaaagc ccgcagcgcc gccggagctg tcgaacttca tcgtgtagcc gttcggcgca
     2641 tcgatatcga cgagctcgac cgcgcccttg aacctcgact tcacgggacc gatcgccgca
     2701 ttcatcacga cctcgtagcg gttctcgccc agtgcggcaa agccctcgca ccccggcacg
     2761 cacatcttca gcatctcgat atcgttcagt gccgtccatg cggtcatgcg atcggccgtg
     2821 agtacctgtt cgcctttcag ttccacggtt cctgcttccc ggttcttggt gttgttcgcg
     2881 acagcgttcg cgccagctcg tgcaggctgt cgatattgtg cacgggaaga aactcgtcca
     2941 cgtgcggcag catcgcgcgg atgccgcgcg ccttgggctc gaacgcctcg tagcgcagca
     3001 gcggattgag ccagaccagg cgccggcacg atttcgacag gcgctccatc tcgcgcgcga
     3061 gcagctcggt gtcgccgtgc tccagcccgt cggtcaccag cagcacggtc gggctgcccg
     3121 aaagcacgcg ccgcgcccag tgcaggttga aatcgtgtat cgcgtgcgcg atgcgcgtgc
     3181 cgcccgacca gtcgtcgacc gcctgcacca cggcggcaac cgcgacatcg ggatcgcgcg
     3241 acgcgagctg gcgcgtgatc ggcgtcaacc gcgtgccgaa cacgaacgcc tgcgtgcgat
     3301 gctcggagcc gctgccgatg ccgaccagcg cgtgcagaaa gtgcaggaac atgcgcgagt
     3361 agcggctcat cgagcccgag atgtcgacga tcaccaccag cggttccggc cgcgtgcggc
     3421 gcgtgcggcg cggcagggcg gcgatgtcgc caccgtggcg cgcggcgtcg cgcagcaggc
     3481 gctgcaggtc gatgcgtgtg ccccgcggcg acggcgcgta gcggcgcgtg atcaggtgcg
     3541 cgaagaacgg ccgcatcgcg tgcagcatct tccgggccgc gttccattcc gcggtcgtca
     3601 tcgtgtcgaa gtcggccttg cgcagcagct cacgttcgga ccacgtcagg tgggcgtcga
     3661 cttcgatgcg ctcctcggcg cgcgcctgct ggcgcgcctg cggactttcc ttgaagagcg
     3721 cctcgccgag gcggcgattc tccggctgcg gaggtgcgcc ggacgcctgc cccacttccg
     3781 gcagcagcag tcgcagcatc tggccgagca gatcgggatc cttccagaag atataaaacg
     3841 cctggtcgaa cagcacgcgg tgctcggcgc ggtcgatcag gcaggcggcg agcgtcgcgt
     3901 ggaaatcctg ccgcgattcg atgccggcca cgcgcagcgc ccgcagcgcg agcagcacgc
     3961 ggtccgtgcc gaccggtatg ccggcgctgc gcagcacgcg cgcgaagtgc acgacgttct
     4021 cggccaggcg tcctggaagc tgttcgaggg ccgcgaccgg caacacgacg caaggcccta
     4081 tgtgacggcg tcgcggcgcg gtcgcgccgc ctcgccgagc cagggcgccg cacgcgcggc
     4141 aagctgcgcg atcgaggccg acaccagcag gcgatgctcg ttcacgaaca gctcgtggtt
     4201 gcgctcgacc tcgttcaacc ggtacacgta gttcgacatg atgccgaacg agcgctcgat
     4261 gccttgcggt gacgtgtcgc ccatccagtt cagccgctcc agcaccgcgc cgttgccgag
     4321 atgaaagtgc gccaccgagt catacggccc gtcgccgcgg cgcagctgcg ccagatagta
     4381 cgcggcggcg ggcagcagca gcgcctcggc accggcggcc gcctcgctgc ggggccactc
     4441 gggagcatcg agtcgttcga acagcgcgcg ccacgcttcg ccctgcgcgc cactgcacag
     4501 gcgctcctgc tggctctgca gccagccgcg aaaacgcggg atcggcgaca gcgtggcaaa
     4561 gcggcggatg ttcggcagtt ccgcgcgcaa ctccgtagcg acctgcttga tcaggaaact
     4621 gccgaacgag atgccgcgca gtccttcctg gcagttggtg accgagtaga acaccgcgca
     4681 gttggccttc gccggcggcg tcacctcgga gtggatgtcg agcagcggct gcaccctcgc
     4741 gctgatgccg cgcgtgagcg cgacctcgat gaagatcagc ggttcgtgcg caagctgcgg
     4801 atgaaagtag gcgaagcagc ggcgatccgc ggcgagccgg cgccgcagat cgtcccagcc
     4861 ttggatctgg tgcacggcct cgtattcgat cagtttctcc agcacgacag ccggggtgcg
     4921 ccagtcgatc cgctcgagcg tgaggaagcc gcggttgaac cacgagcgca gcagatggcg
     4981 caggtcgtcg tcgatcacgc gccagcgcgg atggcgctgc aggccgcgca gcacgcgctt
     5041 gcgcatctgc accagcgccg cggtgccgcc cggcgccatg ttcaggcgcc ggaacagttc
     5101 ctgccgtacc ggctcgacga tctcctgcag gcgcagcaga ttggccggcg acggttccac
     5161 gtcgtagctg cgcgcggcct cgagcaccgc ggacggcgac ggcgagaact cgcgtgccag
     5221 cgcgtcgaag aagcgctcgc gcgcatcctc gtcgagcgcg aggtacgacg ccagcgcggc
     5281 acgcaccgcc gccgcgctcg acacctcgcc gcgctcgcga atcagcttgc ggcagcgacc
     5341 gatcgcctcg cgcaccgtgt gcgcgcgcgc cgcggtcgcg cgcttcatcg cgcttcctgt
     5401 ccggcggtcg gcaccgccag catgccggga cgatcgatcc agtacgcggc cagcacgatc
     5461 agccactgcg cctgcccgac ccacgcgatc gcctgcaccg acggcggcgg cgggccgaac
     5521 aggttgccga agtagattgc caccagcagc gcgagcaacg tccacagcgc gccgcgcggc
     5581 gcgccggtag tggcacgcag atacagcagc acgccgaacg caagcgtgcc gagctccacg
     5641 atcagcgtcg caatcggcga gcgccacagg ccgaagccga tcgccaggtg gccccccggc
     5701 atcagcagca gatcggggcg atgcacgatc gcgtcgagcg cccagtggct cgcgaccagt
     5761 acggcgatga ccaacgcggc gcggcggtcg cgctgccaca ggttatggcc gccgccgagc
     5821 agcagtgccc acacgcccac cgcgagcagg ctgtgcgtcc acgggtaatc ggtgaacgcg
     5881 atcggcgtga cgccgccgag cgacggatcg atgcgcgcct gctccaggcc cagcagcagc
     5941 agcgtcggcc acaccagatc gatccatacc gcggccagga cgagcgtgcc gagcgaggtg
     6001 cctggcgcga acttcttcgc cgccagcgca atgccgtagt gaccgatgaa catcgggcgg
     6061 acgcgtcagg cggcgatttc cgcgcgcacc ttctcgacca gccgcgcggc ctcggtgccc
     6121 tgcatgcggg cgatgtcgtc ctggtacttg agcagcacgc cgagcgtgtt gtcgaccgtc
     6181 tccggctcga gcacgaccgc gtcgagcgcg agcagcgcgc gcgaccactc gatcgtctcg
     6241 gacacgccgg gcagcttgta cagatcgatc tggcgcagcg actgcacgaa cgcgacgatc
     6301 tgtctcgaca gcgcctcctt cgcgtccggc accttgcggt ggacgatctg cagctcgcgc
     6361 gctgcgtccg gatagccgac ccagtgatag atgcagcggc gcttgacggc gtcgtggatc
     6421 tcgcgcgtgc ggttcgacgt gatcaccacc accggcggcg cctcggcgcg gaccgtgcgc
     6481 cactccggga tggtgatctg gaagtcggac agcacttcga gcaggtaggc ctcgaacggc
     6541 tcgtcggtgc ggtccagctc gtcgatcaac agcaccggcg gcaccgcgtg cggcagcaat
     6601 gcacgcagca gcggtcgctc gatcaggaag cgcccggtga acagccccgt cgccacctgc
     6661 tcgcgtgcct cgtgcgtggc ttcggcgagc cgtatc
//
LOCUS       DBNHBY010000053         3944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_814509, whole genome shotgun sequence.
ACCESSION   DBNHBY010000053 DBNHBY010000000
VERSION     DBNHBY010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_814509"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1418)
                     /locus_tag="AC16HB_01295"
     CDS             complement(102..1418)
                     /locus_tag="AC16HB_01295"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5605451.1"
                     /translation="MGERVALQVAVIGAGPMGLAAAYDLAKRGARVVVYERDDRIGGM
                     SAFVDFAGTRIERYYHFICKPDRTTFEYLRDLGLEDRLRWTRTKMGFYFRGRLYDWGH
                     PLALLRFPGLSPVQKLRYGLHVMRAKGITDWAPYDAFSSTAWLHRWIGRQAYDVLWRP
                     LFHFKFYEHEDSLSAAWLGTRIKRVALSRASLFEEELGYLHGGSEVLLEAIAARIRQL
                     GGNIELKTGVDEVLLQDGRVNGVRIAGSTRACDRLISTIPLPYLTRLVPGLPDDERAK
                     VAAIRNVGVVCVLLKLKRPFTRNFWMNINDPRIEIPGLIEYTNLNPLDGSSIVYAPYY
                     MPQTHPKYRRDFREFIDETLRYMTLIRPDFAPAQEVVASTASRYEFAQTVCTPGFYAA
                     LPPMRSKLAGLLMADTSHYSPEDRSISESLRIGAQLAALAMEDGSG"
     gene            complement(1420..2355)
                     /locus_tag="AC16HB_01300"
     CDS             complement(1420..2355)
                     /locus_tag="AC16HB_01300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5605452.1"
                     /translation="MKIVVTGAAGLVGQNLIVLLRECGYRDIVAIDKQAENLALLARL
                     NPGVVTVAADVAQPGAWSERFAGADVVVLLHAQIGGKFAEMFERNNLLGTQLVLDAIR
                     RHAVPFTVHVSSSVVNSVADDNYTRTKKAQEKMVLESGVGCCVLRPTLMYGWFDPKHF
                     GWLARFMARAPVFPIPGHGRYARQPLYERDFCRAILWCIEHRPAGKVYDLVGTERIDY
                     IDIIRAIRDVKNLRTPIVRIPYRLFDLLLRLYALFSDRPPFTSDQLQALTAGDEFSGV
                     DIEATFGFKPTPFADGIRETFCHPVYSQYVVARAE"
     gene            complement(2367..2915)
                     /gene="rfbC"
                     /locus_tag="AC16HB_01305"
     CDS             complement(2367..2915)
                     /gene="rfbC"
                     /locus_tag="AC16HB_01305"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR5605453.1"
                     /translation="MKVIATALPEVLILEPRVFTDERGFFMESWNARTFHAATGIDAK
                     FVQDNHSRSVRNVLRGIHYQVVKPQAKLVRVVSGAVLDVAVDLRRSSPRFGRWVAIEL
                     SAENRRQMWIPIGFGHAFLVRSEYADFLYKTTEYWIAEHDRALRWNDSALSVDWQLGA
                     AAPIVAERDAQAPTLATAEVFA"
     gene            complement(2935..3816)
                     /gene="rfbA"
                     /locus_tag="AC16HB_01310"
     CDS             complement(2935..3816)
                     /gene="rfbA"
                     /locus_tag="AC16HB_01310"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR5605454.1"
                     /translation="MKRKGIILAGGSGTRLYPVTQVVSKQLLPIYDKPMVYYPLTTLM
                     LAGIQDILIISTPADTPRFQDLLGDGHQWGINLRYAQQPSPDGLAQAFLIGREFVGRA
                     PAALILGDNIFYGHDLVEQLRRANARAAGATVFAYHVNDPERYGVVEFDRSGRAVSIE
                     EKPARPRSSYAVTGLYFYDNRVLDIAASIRPSARGELEITDVNQMYMKSGSLQVEIMG
                     RGMAWLDTGTHESLLQASVFIETIEQRQGLKVACPEEIAFRAGWISAEDVARLAEPLK
                     KNGYGQYLLRLLDERVM"
BASE COUNT          685 a         1294 c         1299 g          666 t
ORIGIN      
        1 tgaaccagag gctgaacccg aagcgcgatc cgtaattggc gagcgcggcc agcccgccgg
       61 caactagaaa caacgcaaag cggcgcgatt cggcgatcga ctcagccact tccgtcttcc
      121 atggccagtg cagcgagctg cgcgccgatg cgcaagcttt ctgatatcga acgatcctcc
      181 ggggagtagt gcgaagtgtc ggccatcagc agtcccgcga gcttgcttcg catcggcggc
      241 agcgcggcgt agaagcccgg cgtgcaaacg gtctgtgcaa actcgtagcg cgatgcggtc
      301 gatgcgacca cctcctgcgc cggcgcgaag tccggccgga tcagcgtcat gtagcgcagg
      361 gtttcgtcga tgaattcgcg gaagtcgcgc cggtacttcg ggtgcgtctg gggcatgtag
      421 tacggcgcgt agacgatcga gctgccgtcg agcggattga ggttggtgta ctcgatcagc
      481 ccgggtatct cgatccgggg atcgttgatg ttcatccaga agttgcgcgt gaacggccgc
      541 ttgagcttca gcagcacgca caccacgccg acattgcgaa tggccgccac cttggcgcgc
      601 tcgtcgtccg gcaggcccgg gacgagccgc gtcaggtacg gcagcggaat cgtcgagatc
      661 aggcgatcgc aggcgcgggt gctgcccgcg atgcgcacgc cgttcacgcg gccatcctgc
      721 agcaggactt cgtccacgcc ggtcttcagt tcgatgttgc cgccgagctg cctgatgcgc
      781 gccgcaatcg cctcgagcag cacttcggag ccgccgtgca ggtagcccag ctcctcctcg
      841 aacaggctcg cgcgcgacag cgcgacgcgc ttgatgcgcg ttcccagcca ggcggcggac
      901 agactgtcct cgtgctcgta gaacttgaag tgaaacagcg gtcgccacag cacgtcatag
      961 gcctgccggc cgatccatcg gtgcagccaa gcggtcgacg agaacgcgtc atagggcgcc
     1021 cagtcggtga tgcccttggc gcgcatcacg tgcagtccgt agcgcagctt ctgcaccggg
     1081 gacagcccgg ggaaccggag cagtgcgagc ggatggcccc agtcgtacag gcgtccccgg
     1141 aagtagaacc ccatcttggt ccgggtccag cgcagccggt cttccagacc caagtcgcgc
     1201 aggtactcga atgtggtgcg gtccggcttg cagatgaagt ggtagtagcg ctcgatgcgc
     1261 gtgccggcga agtcgacgaa tgcggacatg ccaccgatgc gatcgtcgcg ctcgtagacg
     1321 acgacacggg cgccgcgctt ggccaaatcg taggccgcgg cgagccccat cggccccgca
     1381 ccgatcaccg cgacctgcag cgccacccgt tcgcccatgt cattcggcac gcgccaccac
     1441 gtactgcgag tacacggggt ggcagaaggt ctcgcggatt ccgtccgcaa acggggtcgg
     1501 cttgaagccg aaagttgcct cgatgtcgac ccccgagaat tcgtcgcccg cagtcagcgc
     1561 ctgcaactgg tcggatgtga acggcggccg gtcgctgaac agcgcgtaca ggcgcagcag
     1621 cagatcgaac agccggtacg gtatgcgcac gatcggcgtg cgcaggttct tcacgtcgcg
     1681 gatcgcgcgg atgatgtcga tgtagtcgat ccgctcggtc ccgacgaggt cgtacacctt
     1741 gcccgccggc cgatgctcga tgcaccacag gatcgcccgg cagaaatcgc gctcgtacag
     1801 cggctgccgc gcatagcggc cgtgacccgg aatcggaaac accggcgcgc gcgccatgaa
     1861 acgcgccagc cagccgaaat gcttgggatc gaaccagccg tacatcagcg tcggccgcag
     1921 cacgcagcag ccgacgccgc tctcgagcac cattttctcc tgcgccttct tggtccgcgt
     1981 gtaattgtcg tcggcgaccg aattgaccac ggacgagctg acgtgcacgg tgaacggaac
     2041 cgcgtgccgg cgaatcgcgt cgagcacgag ttgcgtgccg agcaggttgt tgcgctcgaa
     2101 catctcggca aacttgccgc cgatctgcgc gtgcagcagc acgacgacgt ccgcgcccgc
     2161 aaagcgctcc gaccaggcgc cgggctgcgc cacgtccgct gccaccgtga ccacgcccgg
     2221 attcaggcgg gccagcagag cgaggttctc ggcctgcttg tcgatcgcga cgatgtcccg
     2281 atagccgcac tcacgcagca gcacgatcag gttctggccc accaggccgg cggcgccggt
     2341 gacgacgatc ttcatcgagc cgccgctcag gcgaagactt cggccgttgc gagtgtcggc
     2401 gcctgcgcgt cgcgttcggc gacgatcggc gccgcggcgc cgagttgcca gtcgaccgac
     2461 aacgcggaat cgttccagcg cagcgcgcgg tcgtgttctg cgatccagta ttccgtggtc
     2521 ttgtatagaa agtccgcata ttcgctgcgc accaggaacg catggccgaa tccgatcgga
     2581 atccacatct ggcgcctgtt ttcggcggaa agctcgatcg cgacccacct tccgaagcgc
     2641 ggcgaggaac ggcgcaggtc gacggccacg tcgaggaccg cgccgctgac gacgcgcacc
     2701 agcttggcct gcggcttgac gacctggtag tgaatgccgc gcagcacgtt gcgcaccgaa
     2761 cgcgaatgat tgtcctgcac gaacttcgca tcgatgccgg tcgcagcatg aaaagtgcgc
     2821 gcgttccagc tctccatgaa gaatccgcgt tcgtcggtga atacccgcgg ctcgaggatc
     2881 agcacttcgg gcagtgccgt ggcgatgact ttcatccgga ctctttgctg ggcgctacat
     2941 cacccgctcg tcgagcaggc gcagcaggta ctgtccgtag ccgttcttct tcagcggctc
     3001 ggccagccgc gcgacgtcct cggcgctgat ccagccggcc cgaaacgcga tctcctcggg
     3061 acaggcgacc ttcagtccct ggcgctgctc gatggtctcg atgaacaccg acgcctgcag
     3121 cagcgattcg tgcgtgccgg tatccagcca cgccatgccg cggcccatga tttccacctg
     3181 cagcgatccg gatttcatgt acatctggtt gacgtccgtg atctccagct cgccgcgtgc
     3241 cgatgggcgg atcgacgcgg cgatgtcgag cacgcggttg tcgtagaagt acaggccggt
     3301 gactgcatag ctggatcgcg gccgcgcggg cttttcctcg atgctgacag cgcgcccact
     3361 gcggtcgaac tcgaccacac cgtagcgctc ggggtcgttc acgtggtagg cgaacacggt
     3421 cgcgccggcg gcgcgcgcgt tggcgcgccg cagctgctcg acgaggtcgt gcccgtagaa
     3481 gatgttgtcg ccgaggatca aggcagcggg ggcgcggccg acgaattcac ggccgatcag
     3541 gaacgcctgc gccagcccgt ccggggaggg ctgctgggcg tagcggaggt tgattcccca
     3601 ctggtggccg tcgccgagca aatcctggaa gcgcggcgtg tcggccggtg tcgagatgat
     3661 caggatgtcc tgtatgccgg ccagcatcag cgtggtcagt gggtagtaga ccatcggctt
     3721 gtcgtagatc ggcagcagtt gtttcgacac gacctgggtg accggataca gccgcgtgcc
     3781 cgagccgccg gccaggatga tccctttgcg cttcatcgtc cgtccagcgg cgcgaggcgc
     3841 gccgagtagt tggtctgctc ccagtcgcgg tagctgccgc tgcgcgcgcg acggacccac
     3901 tcgggatgat cgagatacca gcgcaccgtg cgacggatgc cgtc
//
LOCUS       DBNHBY010000054         2954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1085545, whole genome shotgun sequence.
ACCESSION   DBNHBY010000054 DBNHBY010000000
VERSION     DBNHBY010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1085545"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="AC16HB_01315"
     CDS             complement(<1..1331)
                     /locus_tag="AC16HB_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5605455.1"
                     /translation="MPRLLRHALISTRDLAVSAAPFVVLALLLLWGAFLVLNPTPPRK
                     VVLATGPEQGAYAEFGKRYVAELKRYDIAVELRSTQGAAENLKLLRDPSSGVDLAFIQ
                     GGAGDALYAVDEADQDAGLVALGNLFYEPVWVFYRTAAVKGAALTQLTQLRGLRVNIG
                     AEGSGAANLVAKLLHANRIEPAELKIGRLAPTPAVVALLDGEIDALVFVSAPESPLVR
                     MLLQTPGIGLLDFAQADAYARQFAFVDAMPLPRGVVDLARDIPPRDVRLIAPTSTLVA
                     RESTHSAVIQLFVQAARRIHGGTSWFSAVGQFPNSAVTEFPLAREAERFYRGGPPLLQ
                     RYLPFWLANLIDRMWVVLVSIIAVMIPLSRVVPPLYEFRIRSRIFRWYGQLRDIEDSA
                     GGADAQPARLLERLDALDAKVEHIHVPLSYADELYALRSHIEMVRGRLRSAP"
     gene            complement(1372..2799)
                     /locus_tag="AC16HB_01320"
     CDS             complement(1372..2799)
                     /locus_tag="AC16HB_01320"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5605456.1"
                     /translation="MSLSLNVVILLYLVASICFIQALKGLSHPTTSRAGNVFGMVGMA
                     IAMVTTVALVYKLAPQIATLGMGWILLGLVIGGAVGATIAFKVEMTKMPEMVAFMHSM
                     IGLAAVFIAIAAVAEPWAFSIVEKGGPIPMGNKVELFIGAFVGAITFSGSVIAFGKLS
                     AKYKLRVFQSAPVVFAGQHWLNLLLALVMIAAGIWFVATQAWLPFWIMLAIAFALGVL
                     IIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNSMLIIAGSLVGSSGAILSYIMCRA
                     MNRSFFNVILGGFGGEVAGPAAGAQQRPVKSGSPDDAAFLLANAESVIIVPGYGLAVA
                     RAQHPLKEMADKLRAKGVNVRYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINPD
                     FSDTDVALILGANDVVNPAAKDPKSAIAGMPILEAFKARQVIVNKRSMASGYAGLDNE
                     LFYMDKTMMVFGDAKKVIEEIGKAV"
     gene            complement(2808..>2954)
                     /locus_tag="AC16HB_01325"
     CDS             complement(2808..>2954)
                     /locus_tag="AC16HB_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HIR5605457.1"
                     /translation="LTETGLGKSMGVMAVALAAVNVFGGFLVTRRMLEMFKKKEPKAS
                     AEKK"
BASE COUNT          536 a         1019 c          956 g          443 t
ORIGIN      
        1 ggcgcggaac gcaggcgacc gcgaaccatc tcgatgtggc tgcgcagcgc gtacagctcg
       61 tccgcatacg acagcggcac gtggatgtgt tccaccttgg cgtcgagagc gtcgagccgc
      121 tccagcagcc gcgccggttg cgcgtctgca ccacccgcgg aatcctcgat gtcgcgcaac
      181 tgcccgtacc agcggaagat gcgcgagcgg atgcggaact cgtacagggg cggcaccacg
      241 cgcgacagcg ggatcatcac cgcaatgatc gacaccagca ccacccacat gcggtcgatc
      301 aggttggcga gccagaacgg caggtagcgc tgcagcagcg gcggaccgcc gcggtagaag
      361 cgctcggcct cgcgcgccag cggaaattcg gtgacggccg agttcggaaa ctgtccgacc
      421 gcgctgaacc aggacgtccc gccgtggatg cggcgcgccg cctgcacgaa gagttgaatg
      481 acggcggagt gcgtgctctc gcgcgcgacc agcgtcgagg tcggcgcgat cagccgcacg
      541 tcgcgcggtg ggatgtcccg cgccagatcg accacgccgc gcggcagcgg catcgcgtcg
      601 acgaatgcga actggcgcgc atacgcatcg gcctgagcaa agtcgagcag gccgatcccc
      661 ggcgtctgca gcagcatccg caccagcggc gattcgggcg ccgagacgaa cacgagcgca
      721 tcgatttcgc catccagcag cgcgaccacc gccggcgtcg gcgcgagccg gccgatcttc
      781 agttccgccg gctcgatgcg gttggcgtgc agcagcttgg ccaccaggtt cgccgcgccg
      841 ctgccttcgg cgccgatgtt cacacgcagg cccctgagct gcgtgagctg tgtcagcgcc
      901 gcgcccttga ccgccgcggt gcggtagaac acccacactg gctcgtagaa caggttgccg
      961 agcgcgacca gcccggcgtc ctggtcggcc tcgtcgactg cgtacagcgc gtcgccggcg
     1021 ccaccctgga tgaacgcgag atccaccccc gatgacggat cgcgcagcaa cttcaggttc
     1081 tccgccgcgc cctgcgtgct gcgcagttcg accgcgatgt cgtagcgctt cagttccgcg
     1141 acgtagcgct tgccgaattc cgcgtacgca ccctgctccg gaccggttgc cagcacgacc
     1201 ttgcgcggtg gcgtggggtt gagcacgaga aacgcgcccc acagcagcag cagcgcgagc
     1261 acgacgaacg gcgcggcgct gaccgcgagg tcgcgcgtgg agatcagcgc atggcgcagc
     1321 aggcgcggca tggggacaat gatcagccgc ggcgacggaa agttccgacc gtcagacggc
     1381 cttaccgatc tcctcgatca ccttcttcgc gtcgccgaag accatcatgg tcttgtccat
     1441 gtagaagagc tcgttgtcca gcccggcgta gccggaggcc atcgagcgct tgttcacgat
     1501 cacctggcgc gccttgaatg cctcgaggat gggcatgccg gcgatcgccg atttcggatc
     1561 cttggcggcc ggattgacca cgtcgttcgc gccgaggatc agcgcgacgt cggtgtcgga
     1621 aaagtcggga ttgatgtcct ccatctcgaa cacctggtcg tacggcacct cggcttctgc
     1681 gagcagcacg ttcatgtgtc ccggcatgcg gcccgcgacc ggatgaatcg cataacggac
     1741 gttgacgccc ttggcacgca gcttgtcggc catctccttc agcgggtgct gcgcgcgtgc
     1801 caccgcgagg ccatagcccg gaacgatgat cacgctctcc gcgttcgcca gcaggaacgc
     1861 ggcatcgtcc ggcgagccgc tcttgaccgg acgctgctgc gcgcccgccg ccggccccgc
     1921 gacctcgccg ccgaagccgc cgaggatcac gttgaagaac gaccggttca tcgcccggca
     1981 catgatgtac gacaggatcg cgccgctcga gccgaccagc gaaccggcga tgatcagcat
     2041 cgagttgttc agcgaaaacc cgattcccgc cgcggcccag cccgagtagc tgttgagcat
     2101 cgacaccacc accggcatgt cggcgccgcc gatcgggatg atgatcagca cgccgagcgc
     2161 gaaggcgatc gccagcatga tccagaacgg cagccacgcc tgcgtggcaa cgaaccagat
     2221 gccggcggcg atcatcacga gcgcgagcaa caggttgagc cagtgctgcc cggcgaacac
     2281 caccggggcg ctctggaaca cgcgcagctt gtacttggcc gagagtttcc cgaacgcgat
     2341 caccgagccg gagaacgtga tcgcgccgac gaacgctccg atgaacagct ccaccttgtt
     2401 gcccatcggg atcggcccgc ccttctcgac gatcgagaag gcccacggct cggccaccgc
     2461 ggcgatggcg atgaacaccg cggccaggcc gatcatcgaa tgcatgaagg cgaccatctc
     2521 gggcatcttg gtcatctcga ccttgaacgc gatcgtggcg cccaccgcgc cgccgatcac
     2581 gagcccgagc aggatccagc ccatgccgag cgtggcgatc tgcggcgcca gcttgtagac
     2641 caacgccacg gtcgtgacca tcgcgatcgc catgccgacc atgccgaaca cgttgccggc
     2701 gcgcgaggtg gtcggatgcg aaaggccctt gagcgcctgg atgaagcaga tcgaggcgac
     2761 gagatacagc aggatgacga cgttgaggct gagcgacatg acggctccta cttcttctcg
     2821 gccgaggcct tcggctcttt cttcttgaac atctcgagca tccgccgcgt gacgaggaag
     2881 ccgccgaaca cgttgacggc ggcgagcgcc acggccatca cacccatgct cttgccgagc
     2941 ccggtttcgg tcag
//
LOCUS       DBNHBY010000055         9746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_814609, whole genome shotgun sequence.
ACCESSION   DBNHBY010000055 DBNHBY010000000
VERSION     DBNHBY010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_814609"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="AC16HB_01330"
     CDS             <1..1159
                     /locus_tag="AC16HB_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR5605458.1"
                     /translation="TTMALVRMIGALLKDREVGPRIVPIIADEARTFGMADLFRQIGI
                     YSPLGQLYEPEDRDQVSYYKEARNGQILEEGITEAGAIASWVAAATSYSAHGHAMLPF
                     FIYYSMFGFQRVGDAIWAAADSRSRGFLLGATAGRTTLAGEGLQHQDGTSHLVASTIP
                     NCRAYDPCFAYELAAILDDGMHRMLGTNEDVFYYVTVMNENYPQPSQPDGVREGILRG
                     MYRARASARGAAHVRLLGAGTILREALFAAELLEEEFNVAADVFSVTSYAELRRDAMA
                     IERERRLGQRGDTPWIEQQLPADGTPIVAASDYVSALPDLIRPWVRDRMISLGTDGFG
                     RSDLRASLRRFFEVDRHAIAAAALGTIDPALALQAAARFGFDANCAPPWTR"
     gene            complement(1160..1792)
                     /locus_tag="AC16HB_01335"
     CDS             complement(1160..1792)
                     /locus_tag="AC16HB_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5605459.1"
                     /translation="MNVRLADAGVRRDAAPLSLALASAIDPRAVRQVLGVLPVPALLL
                     DGERAICHVNAAAAPHLNDPLCIVIAGGRVCRFAGASSEPFNAVFQRAFDGQSSVVAV
                     TVVSGQRCTLWRVSLAPLHARGDARRVIVLLEPPLSSLRALPLLERLFGLTPAEARVL
                     ALLLDDRRPKEIARALDIEVTTVRSHLKSLFLKTGTHRQGELIGLALSAA"
     gene            complement(1905..2246)
                     /locus_tag="AC16HB_01340"
     CDS             complement(1905..2246)
                     /locus_tag="AC16HB_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605460.1"
                     /translation="MSAPDSGNGVARSDAGRPRTSGSSKHQSGKNVEQEASMQSRTQD
                     RFQNRRRSNGGNKDSFWGFAVLAVFIVTTLSFAVSVYVDSRTEFLSGGIADESSAYTL
                     AHAIVALAGAH"
     gene            complement(2243..2578)
                     /locus_tag="AC16HB_01345"
     CDS             complement(2243..2578)
                     /locus_tag="AC16HB_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605461.1"
                     /translation="MKRITFAALAVAMSCAFAPVVHAEDGKYPQSVMGEGVSVKTRAQ
                     VIAEMSEPAAAEASTAKTRAQVIAETREAARLGLLPGEREREATAEQERLIRLAGERA
                     VESQTVAKH"
     gene            complement(2798..3880)
                     /locus_tag="AC16HB_01350"
     CDS             complement(2798..3880)
                     /locus_tag="AC16HB_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5605462.1"
                     /translation="MKSRAELAHIRLLCSLGLGGAAIMPALLKSLHQLVPCDSAGFFW
                     VDRDGDMADMYVERMLPPELTQRYFERHYDSAAHAFRERLLARMARREYIGEVVVDSR
                     LEETPYFQEILRPLGVQQILIAVVHDEDVPLGQLSLYRGAYAPRFTGDDRDALATASR
                     YMAPALCTPIATQPDERDQSFRDSDEEAVVVFDAAGTISSASHRAHALLAHASGEAVN
                     RNTMRGAIARAVRKLLVGLAEQLQLNAPEQSVASVVVSNVWGRFRLRAYTLEGGQYAA
                     LVQRQEHFLVRLADAMRAVSLSPQQRETALLLARGMSNNEIAAAMSVSVNTANYHVKQ
                     LFQKLDAHDRGEVLVRIMDAGAAHRA"
     gene            4361..6223
                     /locus_tag="AC16HB_01355"
     CDS             4361..6223
                     /locus_tag="AC16HB_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5605463.1"
                     /translation="MAALVPSTDLRYIAKTIMFADVVESVRLIQLDEQGAATRIRTFL
                     RYAAEELVPTYRGTLVEHRGDGLLVTFDEARDAVACATALQRTTQSLATTEPHGSALR
                     IRIGIHTGDVLTDGEKYYGHGVNMASRLMTVAGPQETIVSAFVRDRLTNGLDGDIEDL
                     GLLAEDESVRAPDGALKIESIGEVNLKHVQGPVRAYRVGEVGPDPVVPPGTSEMTDLR
                     PCIAVLPFSHYQGDSLSIGVGDVISDQIISALSLNSGVRVISRLSSQAFRGRSVGLAE
                     TAATLGASYVLSGRYWEGASTLTVNVELAAVRSGQVEWADSFSCTTADVLNPNGDCVA
                     RIASGVMNSIVRSELRVARALALPNLDSHTLYLAATVLLHRFSRADFERAHAMLLALQ
                     ERAPRHAHPLAWLARWHVFRAVQGWSTDMDSDRQEALSYAHRAIDIDPESSLALTIAG
                     SVHVNMLKDLDGALSLYRQALDINPNESLAWILLGTAQTFRGSGDEALVSCRRGIDLS
                     PIDPIRFFYDNLAAGAAVVAQNYRLGLQYAERSVRANRLHSSSLRTLAICQGMLGLKV
                     AAQESVRQLLRIDPTLTAETYITRLPFADQRLAHRMATALVQAGLPAGAFVPEP"
     gene            6237..7319
                     /locus_tag="AC16HB_01360"
     CDS             6237..7319
                     /locus_tag="AC16HB_01360"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5605464.1"
                     /translation="MAGFGPYEGLEGLEGLEGLEGLEGLEGLAVGRQARAGVANALVR
                     SREGYLGFRPQVTDAPLPPIKATGTFPPAGVNDDLLRLGPGVRRRIWCAELSASVDVK
                     PNATGVEVNYSPANAPSAIAELERPLIATFNAQTDLVLSWAELRAERMPEILSQLSVP
                     LPFWASLLNLNNTLTPRTIDLLKVSLDFAASTIHRCKHILNCPRPGVYSTRVQPPIDT
                     PLFSAFPSGHATEAFMTARLLQLLLEDTSNPRNELAMQLDGLADRIAANRVVAGVHFP
                     IDSAAGWVLGTCLAEFIRAKCVDQTKWQPIKFVQQNWTGSDDFRLDETKTHQAYDRST
                     PQQGPAAGVKGSALEWLWDKARSEWK"
     gene            7340..9253
                     /locus_tag="AC16HB_01365"
     CDS             7340..9253
                     /locus_tag="AC16HB_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5605465.1"
                     /translation="MTSRLHWVDAKDGGLGVRVDWPALLKSGRQVDPYLAWDDLKYLA
                     RTGQERGTDQSFPVVLEHSRTPGHTPRFETLWLDKNQLIAQLNQLLVKGGAVERLQLA
                     AIRSPVQNVRVGTVNYPPPARELRTLRILGVIDDWCPFAHPALAPQPNSAELGGVEFL
                     WHQSGKSASGNAVPSPPWHAPAGKFTYGAQLDGLAIRAAIKQAAAKFGASRTVEEAVY
                     RELGYADLHHGSKHGVGILHAAAGELRSERGKESALDGVPIVFVQFPTAAVEDTSGGW
                     MGLYAVDAIDYIIERAEFLRGVALARDVEVTIVLSYGATAGPHDGTSVVEAAIEERLS
                     QGNGNVRVVLAAGNNYGKRIHASRKLSGSGGSVEFLVLVPPDKATETFVEFWLPWLAA
                     PGDVEVTVTDPSGAASTQLAVGDSRALREPGKPYAAGVILSQGVAQGDDKTMILLAVA
                     PTRSLPGGTGPAGIWKVRIKPTTVHLPASACVHAWVERDDVITGLRRTQQARFVDDGT
                     GYVPDVSDPRDTGAVDLYTLNSIANSPGTVVVGGYRICDRTVAGYSSAGPTSADLRSK
                     LDFSAPSDVGIATRGILTGGSFSGSTRRMSGTSIAGPIVARRMIQNAIPKMLPDDIRG
                     DQRRRGPRVENVP"
     gene            complement(9327..>9746)
                     /locus_tag="AC16HB_01370"
     CDS             complement(9327..>9746)
                     /locus_tag="AC16HB_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605466.1"
                     /translation="RLSLEREKPRGYDVLGIDAFSGDSVPMHLMTREAMALYARHLAP
                     NGVIVFQATNRYIDMMPVVKRLAAEQGMEAVLVSDTPESQTGADYWLSSTDQILVSRD
                     RKLLAHPRIADASNEIRDRPELPTFTDAHHNLVRILK"
BASE COUNT         1724 a         3151 c         3205 g         1666 t
ORIGIN      
        1 cacgacgatg gcgttggtgc gcatgatcgg cgcgctgctg aaagaccggg aagtcggtcc
       61 gcgcatcgtg ccgatcatcg ccgacgaggc gcgcacgttc ggcatggccg acctgttccg
      121 ccagatcggc atctattcgc cgcttggcca gctgtatgaa cccgaggacc gcgaccaggt
      181 cagctactac aaggaagcgc gcaacggcca gatcctcgag gaaggcatca ccgaggccgg
      241 cgcgatcgcg agctgggtcg ccgcggccac cagctacagc gcgcatggcc acgcgatgct
      301 gccgttcttc atctactact cgatgttcgg cttccagcgc gtcggcgatg cgatctgggc
      361 cgcggccgat tcgcgctcgc gcggctttct gctcggcgcg accgcaggcc gcaccacgct
      421 ggccggcgaa gggctgcagc accaggacgg cacgagccat ctcgtcgcga gcacgattcc
      481 caattgccgc gcgtacgacc cgtgctttgc gtacgagctg gcggcgatcc tcgacgacgg
      541 catgcaccgc atgctcggta ccaacgagga cgtgttctat tacgttacgg tgatgaacga
      601 aaactacccg cagccttcgc agcccgatgg cgtgcgcgaa ggcattctgc gcggcatgta
      661 tcgcgcgcgc gcctcggcac gcggcgcggc gcacgtgcgg ctgctcggcg cgggcaccat
      721 cctgcgcgag gccttgttcg cggcggaact gctggaagag gagttcaacg tcgcggccga
      781 cgtgttttcg gtgacgagct acgccgagtt gcgacgcgac gccatggcga tcgagcgcga
      841 gcgccgtctc ggccagcgag gcgacacgcc gtggatcgag cagcaactgc ccgccgacgg
      901 cacgccgatc gtcgccgcga gcgactacgt cagcgcgctg ccagacctga tccggccgtg
      961 ggtgcgcgac cgcatgatct cgctcggcac cgacggcttt ggccgcagcg acctgcgcgc
     1021 gagcctgcgc cgatttttcg aggttgaccg gcacgcgatc gcggcggcgg cgctgggcac
     1081 gatcgatccg gcacttgcgc tgcaggcagc ggcgcgtttc ggcttcgatg caaactgcgc
     1141 gccgccgtgg acgcgctagc taggccgccg acagcgccag gccgatcagc tcgccctggc
     1201 ggtgcgtgcc ggtcttcagg aacagcgact tcaagtggga acgcacggtg gtcacctcga
     1261 tgtcgagcgc gcgcgcgatc tccttcggac ggcgatcgtc gagcagcagt gccagcacgc
     1321 gcgcctcggc cggggtcagc ccgaacaggc gctcgagcag cggcagtgca cgcagtgaag
     1381 acagcggcgg ttcgagtagc acgatcacgc gccgcgcgtc gccgcgggca tggagcggcg
     1441 cgaggctgac gcgccacagc gtgcaccgct ggccgctgac gaccgtcacg gcgacaacgc
     1501 tgctctggcc gtcgaacgcg cgctggaaca cagcgttgaa cggctcgctc gatgcacctg
     1561 cgaagcggca cacgcggccg ccggcgatca cgatgcacag cggatcgttc agatgcggcg
     1621 cggctgccgc attgacgtgg cagatcgcgc gttcgccgtc gagcagcagc gccggcaccg
     1681 gcagcacgcc gagcacctgg cgcacggctc gcggatcgat ggccgacgca agcgccagcg
     1741 acaagggtgc agcgtcgcgc cgcacgccag catcggcgag gcggacattc atcatcacag
     1801 tgtgggggcg gccgagtcag attagcccga acggaacgcg gaagcgtcgc aaccaccgaa
     1861 gcatgcggcg tatcaggacg gcgcccgaca acatcggatg atgctcaatg cgcgccggcc
     1921 aacgcgacga tcgcgtgtgc cagcgtgtag gcgctgcttt catcggcgat ccctccggac
     1981 aggaattcgg tgcgcgaatc cacgtacacc gacacggcaa agctcagcgt tgtcacgatg
     2041 aacacggcca gcacggcgaa tccccagaac gagtccttgt tgccgccgtt gctgcgccgt
     2101 cgattctgaa aacggtcttg agtccgggat tgcatggaag cctcctgctc gacgttcttg
     2161 ccggattgat gcttcgatga gccggaagtg cggggtcgcc cggcatccga ccgggcgacc
     2221 ccgtttccgg agtcaggcgc gctcaatgct tggcgacggt ctggctttcg acagctcgtt
     2281 cacccgccag cctgatcagc cgctcctgct cggccgtggc ctcgcgttcg cgctcgcccg
     2341 gcagcaggcc caggcgcgca gcctcgcgcg tctcggcgat gacttgagcg cgcgtcttgg
     2401 cggtcgaggc ctcggccgcc gcaggttcgc tcatctcagc gatgacctgg gcgcgcgtct
     2461 tcacgctgac gccctcgccc atcaccgatt gcgggtattt gccgtcttcg gcgtggacga
     2521 ccggcgcgaa cgcgcaagac atggccactg cgagtgctgc gaaagtgatg cgtttcattt
     2581 gaatctcctt ttgtatgcaa cgaccagccc gtcgcgccct ggcgtccctt gaggaaccgt
     2641 gcacccggtc ggtcgctggt ctcggccgcg ccaggtgcac tccggaggta cttttgcttt
     2701 tcttgctccg gcgccgcgct gtgacgacat ggtcggcgaa tcggcgcgcg cgcgccccgc
     2761 tcgcgcgggt aggaggttct gtcgaaaatt cgcgcagtca ggcgcggtga gcggcgcccg
     2821 cgtccatgat gcgcaccagc acctcgccac ggtcgtgcgc gtcgagcttc tggaacagct
     2881 gcttgacgtg gtagttcgcg gtgttcaccg acacactcat ggccgcggcg atctcgttgt
     2941 tgctcattcc gcgtgccagc agcagagccg tttcgcgctg ctgtggagac agcgatactg
     3001 cgcgcattgc gtcggccagt cgcacgagga agtgctcctg gcgctggacc agcgcggcgt
     3061 actgaccgcc ctcgagcgtg taggcgcgca gtcgaaagcg accccagacg ttgctgacca
     3121 cgaccgacgc gacggactgc tcgggcgcat tcagctgcaa ctgctccgcg agcccgacca
     3181 gcagcttgcg taccgcgcgc gcgatcgcgc cgcgcatcgt gttgcggttg accgcttcgc
     3241 cgctggcgtg cgcgagcaat gcatgcgcgc gatgcgaggc ggacgaaatg gtgccggcgg
     3301 cgtcgaacac gaccaccgcc tcctcgtcgc tgtcgcggaa ggattgatcg cgctcgtccg
     3361 gttgcgtagc gatcggtgta cacagcgcag gcgccatgta acgcgacgcc gtggccaatg
     3421 catcgcggtc gtcccccgtg aagcgcggcg catacgctcc gcggtaaagc gagagctggc
     3481 cgagcggcac atcctcgtcg tgcacgaccg caatcaggat ctgctgcacg ccgagcggcc
     3541 gcaggatctc ctggaagtac ggggtttctt ccagcctgga atcgaccacg acctcgccga
     3601 tgtactcgcg ccgcgccatg cgcgccagca accgctcgcg gaatgcgtgc gcggcactgt
     3661 cgtaatggcg ctcgaagtac cgctgcgtca gctccggcgg cagcatgcgc tcgacgtaca
     3721 tgtcggccat gtctccgtcg cgatcgaccc agaagaaccc ggccgaatcg caaggaacca
     3781 gctggtgcag ggacttgagc agggccggca tgatcgcggc accgccgagt ccgaggctgc
     3841 aaagcagccg gatgtgggcg agttcagcgc ggctcttcat cgtcgcggcc cgcggttcat
     3901 accagagatc cttcaattga aggaggcgga aatacggctc gctgagcatg ctagccacat
     3961 gcggcaacgg catccacccg gctccgggcg caaatggtcg cccgatcgac gcgaaggtcg
     4021 atctgcttgc accgtgtcgg ggcaaaggtc agccgggatg ccgtgcccca tagcgtgtga
     4081 agaggtccac ccgtcgaatt gcctggggcc ggtttgtgtt gccgtccctc gtacggcgat
     4141 tgccttgccg cctctgatcc ggaacccggc ggatgcggca tccggccgtg cgccccgatc
     4201 aactccgcga aacgcaattt ctccggtttg cccggccttg aatgtgatag aacattcacg
     4261 gcggcattcg gcggggtgcc gggtcgaatg gttgtcctcc gaacgcctgc caccgaggcc
     4321 aaacgaaagg ggggcgctcc ggaaggccgc gcgaaccgac atggccgcac tcgtcccaag
     4381 caccgacctt cgctatatcg cgaagacgat catgttcgcg gacgtcgtcg agtctgtgcg
     4441 tctgattcag ctcgacgaac aaggggccgc cacacgcatt cgcacattcc tccgctacgc
     4501 cgcggaagaa ctcgtgccga cctaccgagg cacgctggtt gaacatcgcg gcgacggtct
     4561 tctcgtcacg ttcgacgaag cccgggacgc ggtcgcatgc gcaaccgctc tccaaaggac
     4621 gacgcagagt ctggcaacga ccgagcctca cggcagcgcg ctaagaattc ggatcggaat
     4681 ccacaccggc gacgtgctca ccgacggcga aaagtactac ggccacggag tcaacatggc
     4741 gtcgcgactg atgacggtcg cgggtccaca ggaaacgatc gtgtccgcct tcgttcgtga
     4801 tcggctgacg aacggactcg acggagacat cgaggacctt ggactccttg cggaagatga
     4861 atcggtccgc gcgcccgacg gagccctcaa gatcgaatcg attggcgaag tcaatctgaa
     4921 acacgtccag ggaccggtcc gggcctaccg cgtcggtgaa gtcgggccag atcctgtcgt
     4981 gccgccgggg acgagcgaga tgacagatct gcggccctgc atcgcggtac tgccgttcag
     5041 ccactaccag ggggattcgc tgtccatcgg ggtgggtgac gtcatttcgg atcagatcat
     5101 cagcgcgctt tcattgaatt ctggcgtgcg cgtgatctcg cggctgtcct cgcaagcctt
     5161 tcgtggtcga tccgtcggac tggcggaaac ggcagcaacg ctcggcgctt cgtatgtact
     5221 cagcgggcgg tactgggagg gcgcgtcgac cctgactgtg aatgtcgagc tggccgcggt
     5281 gcggtccggg caggtcgagt gggccgacag cttctcctgc acgacggccg acgtattgaa
     5341 cccgaacggt gactgtgtcg ctcggattgc gagcggagtg atgaactcga tcgtgcgcag
     5401 cgaactacgc gtggcacgcg cgcttgccct cccgaatctc gatagccaca cgctctacct
     5461 cgctgcgacg gtcctgctgc accggttctc gcgggcagac ttcgagcgcg cgcacgcgat
     5521 gctgctggcg ctgcaggagc gcgcgccccg acatgcccac ccgctggcct ggcttgcgcg
     5581 ctggcatgtc ttccgcgcgg tgcagggatg gtcgaccgac atggacagcg accgccagga
     5641 agcgctgagc tacgcacatc gtgcgatcga catcgatccc gagtcttctc tcgcactgac
     5701 gatcgcggga tccgtgcatg tcaacatgct gaaggatcta gacggcgcat tgtcgctgta
     5761 cagacaggcc ctcgatatca acccgaacga gtcgctggcc tggattctgc tgggtaccgc
     5821 acagacgttt cggggcagcg gcgacgaggc gctggtgtcg tgccgccgcg gcatcgatct
     5881 gtcaccgatc gacccgatca gattcttcta cgacaacctg gccgcgggtg ccgccgtggt
     5941 ggcacagaac taccggctcg gccttcaata cgcagaacgg tcggtgcgag ccaatcgact
     6001 gcacagctcg agtctgcgca ccctggcgat ctgccaggga atgctgggac tgaaagtagc
     6061 ggcgcaggag tcagtcaggc aactcctgag aatcgatccc acgctgacag cagagaccta
     6121 catcacgcgg ttgccgtttg cggaccagcg gttggcgcat cgaatggcga cggcgctggt
     6181 acaggccggt ctgcccgcag gggcgtttgt tccagaaccg taacgcggag gacaaaatgg
     6241 ccggattcgg accgtacgaa ggtctggaag ggttggaggg actcgaggga ctggaagggt
     6301 tggaggggct cgaagggctt gctgtcgggc gtcaggcacg tgcgggcgtc gctaacgcac
     6361 tggtcaggag ccgagagggg tatctgggct tcaggcctca ggtgaccgat gccccactgc
     6421 cgcctatcaa ggcaaccggc acctttccac cagcgggcgt caatgacgat ctgcttcgac
     6481 tgggaccggg agtccgacgc cgaatctggt gcgccgagtt gtccgcgtcg gtcgacgtga
     6541 agccgaacgc caccggggtc gaagtgaact actcaccagc taatgcgccg tcggccattg
     6601 ccgaattgga gcgcccgctc atcgccacat tcaatgccca gaccgatctg gtgctcagct
     6661 gggccgaact gcgcgcggag cgcatgcccg agatcctttc ccaattgagc gtgccgctcc
     6721 ccttctgggc ctcgctcctg aatctgaaca acacgctgac gccgcgcacg atcgatctgc
     6781 tgaaggtcag tctcgatttt gccgcgtcga ccatccatcg gtgcaagcac atcctgaact
     6841 gtccgcgccc gggcgtctat tcaacgcgcg tgcagccgcc gatcgatacg ccgctgttct
     6901 ccgcgttccc gagcggtcac gcgaccgagg cattcatgac cgcacgattg cttcagcttc
     6961 tcctggagga cacatcgaac ccacgcaacg aactggcaat gcaactcgat ggtctcgctg
     7021 accggatcgc agctaatcgc gtcgttgccg gcgtgcactt cccaatcgac agcgcagctg
     7081 ggtgggtgct cgggacctgc ctcgccgagt tcatccgcgc gaaatgcgtt gaccagacga
     7141 agtggcagcc gatcaagttc gttcagcaga actggaccgg cagcgacgac tttcgacttg
     7201 atgaaacgaa gacccatcaa gcatacgacc gcagtacgcc ccagcaaggg ccggcagccg
     7261 gcgtcaaagg ctctgcgctg gaatggctgt gggataaagc gaggagtgag tggaaataga
     7321 ctgaccaccg aggcaccaga tgacatctcg actgcattgg gtggacgcca aggatggcgg
     7381 gctcggcgtg agggtcgatt ggccggcact gctgaagtcc ggtaggcagg tggatccgta
     7441 tctcgcctgg gacgatctca agtacctcgc ccggacgggc caggagcgcg ggactgacca
     7501 gagctttccc gtcgtgctcg aacactcacg cacaccgggt cacacgccac gattcgagac
     7561 actctggcta gacaagaatc aactgattgc acagttgaac cagttgctgg tgaagggcgg
     7621 cgccgtcgag cgcctccagc tggcggcgat cagaagtccg gtgcagaacg tgcgcgtcgg
     7681 tacggtcaac tatccgccgc ctgcgcgcga actgcggacg ttgcggattc ttggtgtgat
     7741 cgacgactgg tgcccgttcg cgcacccggc actcgcaccg cagccaaatt ccgccgagct
     7801 tggcggtgtc gaatttctct ggcaccagag cggcaagagt gcaagcggca atgcggttcc
     7861 gagcccaccg tggcatgcgc cggcgggcaa gttcacgtac ggagcgcaac tcgacggact
     7921 ggcaatccgc gcagcgatca agcaagcggc ggcgaaattc ggtgcatcgc gaacggtcga
     7981 agaagcggtc tatcgtgagc tcggctatgc ggatctccac cacggcagca agcacggcgt
     8041 cggcatcctg cacgcggcgg ccggtgagct gcgctccgag cgcggcaagg agtccgctct
     8101 agacggggtt ccgatcgtct tcgtgcagtt cccgacagcg gccgtcgaag atacttcggg
     8161 cggatggatg ggactctatg cggtcgatgc gatcgattac atcatcgagc gcgcggagtt
     8221 tctccgcggt gtggctctcg cacgggacgt cgaggtgacg atcgttctca gctacggcgc
     8281 cacggccggc ccgcacgacg gcacttcggt ggtcgaggcc gcgatcgagg agaggcttag
     8341 ccagggaaat ggcaatgtga gggtcgtgct ggcggccgga aacaactatg gcaagcggat
     8401 ccacgcctcc cgcaagcttt ccgggtctgg tggttcagtc gagttcctcg ttctcgtccc
     8461 gccggacaag gccaccgaaa cgttcgtcga gttctggctg ccctggttgg ccgctcccgg
     8521 cgacgttgag gttactgtga ccgatccctc cggggctgca agcacacagc tcgcggtcgg
     8581 agacagtcgc gcactacgcg aaccgggaaa gccgtatgcg gcgggggtca tcctttccca
     8641 aggcgtcgcg caaggcgatg acaagacgat gatcctgctt gcggttgccc cgactcgaag
     8701 ccttccgggc ggaaccggtc cggcgggcat ctggaaggtc cggatcaaac cgacgacggt
     8761 gcatttgccg gcatccgcat gcgtccacgc gtgggtcgag cgcgacgacg tgatcaccgg
     8821 gctgcgccgc acgcagcagg cgcgctttgt ggatgacggg acgggctacg tgccggacgt
     8881 ttccgatccg cgcgacacag gtgcggtcga cctctatacg ctcaacagca ttgccaattc
     8941 gcccggcact gtcgtcgtcg gcggttatcg aatctgcgac cggaccgttg cgggctattc
     9001 ctcggccggg cccacgtcag ccgatctgcg cagcaagctc gatttctccg cccctagcga
     9061 cgtagggatt gcgacccggg gcatcctgac cggcggttcg ttctccggtt cgacccgacg
     9121 catgagcgga acgagcatcg cgggacccat tgtcgcccgg cgcatgattc agaacgccat
     9181 cccgaaaatg ctgcccgatg acatccgcgg tgatcaacgt cgaagaggac cgcgagtcga
     9241 gaacgtgcct tgaccactcg gcggcctctg acaccgcgcc gcggcatggc tgcagttgca
     9301 cggccccggt tgaggtcgcg aattcttcac ttcaatatcc gcaccagatt gtgatgcgcg
     9361 tccgtgaatg tcggcagctc gggccggtcg cgaatctcgt tggacgcatc cgcaatgcgc
     9421 ggatgcgcga gcagcttgcg gtcgcgtgaa accaggatct ggtcggtgga actgagccag
     9481 taatccgcgc cggtctgcga ctcgggggta tcggacacca gcaccgcctc catgccctgc
     9541 tcggccgcga gccgcttcac gaccggcatc atgtcgatgt agcgattggt ggcctggaac
     9601 acgatcacgc cgttgggtgc gagatggcgc gcgtacagcg ccatcgcttc gcgcgtcatc
     9661 aggtgcatcg ggaccgagtc gccggagaac gcgtcgatgc cgagcacgtc gtagccgcgc
     9721 ggtttctcgc gttccagcga caggcg
//
LOCUS       DBNHBY010000056         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1220849, whole genome shotgun sequence.
ACCESSION   DBNHBY010000056 DBNHBY010000000
VERSION     DBNHBY010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1220849"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1654
                     /locus_tag="AC16HB_01375"
     CDS             <1..>1654
                     /locus_tag="AC16HB_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR5605467.1"
                     /translation="VLRGPFSALYGNSSGGVIALTTELKPQPFRVELNGAAGENSTWR
                     IGANAVGGTDPYAFAIDATRFDTNGTRPHSAARRDTLNLRWAALESPFGRVRLALNSV
                     SMPDAQDALGLTRAQFDADPDQTAAVAFQFDTRKTTRQTTASFDLESRLSERTTLNAA
                     AWLGTRAVTQFQAIPVATQANPRHPGGVIDFDRNFGGVDIRARWENKDVTATVGVDFE
                     RMDENRRGYENFIGTQLGVQGELRRDERNQVQSIDPYAQVELRWGDAWRLHAGVRASH
                     VEFDSRDRYIVGVNGDDSGSKSFSGVNPTAGIVFRPHPRASLYLSYGRGFETPTLNEL
                     AYRPDGTAGVNTSLAAARSNNFEVGAKLQWTSTLLATVAAYTIETRDDLVVLSNSGGR
                     SGFGNVGKTRRDGAEVALEWRPASRWSAYASASYINARFDAAFLTCGPAPCLAPSVPV
                     AAGNKLPAVPARAGFIELKYRGGWVDLGIQGRAQSSLYVDDRNSDRAAGYAVVALQAS
                     RSFNLAARRAKAFVRVDNALDRRYAGSVIVNEANGRFYEPAPG"
BASE COUNT          269 a          550 c          551 g          284 t
ORIGIN      
        1 aggttctgcg cggcccgttc tcggcgcttt acggcaattc gtcgggcggc gtgatcgctt
       61 tgacgaccga actcaagccg cagccttttc gcgtcgaact gaacggcgct gccggcgaga
      121 attccacctg gcgcatcggc gccaacgcgg tcggcggcac cgatccctat gcgttcgcca
      181 ttgatgcaac ccggttcgac accaatggca cgcgtccgca cagcgcggcg cggcgcgaca
      241 cgctgaacct gcgctgggcc gcgctcgaat cacctttcgg gcgggttcgc ctcgcgttga
      301 attcggtatc gatgcccgac gcgcaggatg cactcgggct gacgcgcgcg cagttcgatg
      361 cggatccgga ccagaccgct gcagtggcct tccagttcga cacgcgcaag acgacgcggc
      421 agacgacggc gagcttcgac ctcgagtcgc gcctgtccga gcgcacgacc ctgaacgcgg
      481 cggcatggct cgggacgcgc gcggtcacgc agttccaggc gatcccggtg gcgacgcagg
      541 ccaatccacg ccatcccggt ggcgtgatcg atttcgatcg aaacttcggc ggcgtcgaca
      601 ttcgcgcacg ctgggaaaac aaggacgtga ccgccactgt cggcgtcgac ttcgaacgca
      661 tggacgagaa tcggcgcggc tacgagaact tcatcggtac gcagctcggc gtccaaggcg
      721 agctgcgtcg cgacgaacgt aaccaggtcc aatcgatcga tccgtatgcg caagtggagc
      781 tgcgctgggg cgatgcatgg cggctgcacg cgggcgtgcg cgcttctcac gtcgagttcg
      841 actcgcgcga ccgctatatc gtcggcgtga acggcgatga ctcgggcagt aagtcgtttt
      901 ccggggtaaa tccgaccgcg ggcatcgtct ttcgccccca tccgcgggcg tcgctgtatc
      961 tgtcctatgg ccgcggcttc gaaacgccga cgctgaatga actcgcgtac cgtcccgacg
     1021 gcactgccgg cgtgaacacg agtctcgctg cggcgcggag caacaacttc gaggtcggcg
     1081 cgaaactgca gtggacctcg acgctgctcg ctacggtcgc cgcgtacacg atcgaaaccc
     1141 gtgacgatct ggtcgtgctg tcgaacagtg gcggccgcag cgggttcggc aacgtcggca
     1201 agacgcggcg cgacggcgcg gaggtcgcgc tcgagtggcg gccggcgtca cgttggtcgg
     1261 cctatgcaag cgcctcctat atcaatgcac gcttcgacgc cgcgtttctc acctgcggac
     1321 cggcgccctg cctcgcgccg tcggtgccgg tcgcggccgg caacaagcta cctgcggtac
     1381 ccgcgcgcgc tgggttcatc gagctcaagt atcgcggcgg ctgggtcgac ttgggcatcc
     1441 agggccgcgc gcagtcgtcg ctgtatgtgg acgatcgcaa cagcgaccgc gcggctggct
     1501 acgcggtcgt tgcgcttcag gcttcgcgca gcttcaactt ggccgctcga cgggcaaagg
     1561 cctttgttcg cgtcgacaac gcgctcgatc gccgctacgc cggctcggtg atcgtgaatg
     1621 aagccaacgg ccgcttctat gagccggcgc cggg
//
LOCUS       DBNHBY010000057         6272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_341408, whole genome shotgun sequence.
ACCESSION   DBNHBY010000057 DBNHBY010000000
VERSION     DBNHBY010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_341408"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="AC16HB_01380"
     CDS             <1..239
                     /locus_tag="AC16HB_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605468.1"
                     /translation="RPGPVPDSIESSIPGRFEGWVGNSRFRLSNGQVWEIRDGSQASY
                     DLQDPKVRIVRGLSGTFFIEIAGVSQTPRVRRVE"
     gene            343..858
                     /locus_tag="AC16HB_01385"
     CDS             343..858
                     /locus_tag="AC16HB_01385"
                     /inference="COORDINATES: protein motif:HMM:NF022893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HIR5605469.1"
                     /translation="MSRLPVLFLLSLTAVASVARAEVNVSFLAPERFTDTARDGGFGR
                     PDKGKTLDEFKRHLAGLGERCIPPGQSLDIVIRDIDLAGQQEPRRGADGEFLRVLKGV
                     SNPRIDLEYVWRSADGTVLGKGAERVAEASFLPERTHDPRTHDSLYYEKAMLTQWFEA
                     RFCKSTEAPKK"
     gene            complement(887..2098)
                     /locus_tag="AC16HB_01390"
     CDS             complement(887..2098)
                     /locus_tag="AC16HB_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5605470.1"
                     /translation="MNDKGTGRCVRVLLAVAAALTLNIAHAQGLRPEVGKPLQQAAEL
                     LKAGRAKEALAKVREAENVPNKTPAEQLMLDRMRGAAAFRAGDPATAIRSFEAAYASG
                     RLSTGEQAQVAEQLAFAFSQVKDWNKTREWAAKAQQAGGSSPQLNQLLSYVSAQSGDY
                     NAIARDAQAAISAAEQGGRPQEGDLLRLADAQMRLGNTQGQAATIEKLISYYPKKEYF
                     ALYLNRLIGRSGFSDRHALDVMRLKLAIGSLTKAEDVMEMAQLALQAGQAGEAKKVLD
                     DAFASGLLGKTGEVERQKRLLALATQKAAAAPADLKEAETEGMAARDGNVLVRTGLAY
                     TGLGQYDKGIELIQKGIAKASLRNPNDAQLHLGIALTRAGQKGKAAQAFKSVGGSDGA
                     ADLARMWMRLP"
     gene            complement(2095..2526)
                     /locus_tag="AC16HB_01395"
     CDS             complement(2095..2526)
                     /locus_tag="AC16HB_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5605471.1"
                     /translation="MAMNVGPGVGSSEPEVMIDINTTPLIDVMLVLLVMLIITIPIQL
                     HAVNLNMPVGNPPPPLEKPVVVTIDVDFDGTIFWNGDIVPNKDALEARLTDAAAAVPQ
                     PEVHLRPNKLAEYKDVAAVMAAAQRLGVTKLGLVGNEQFVK"
     gene            complement(2555..2983)
                     /locus_tag="AC16HB_01400"
     CDS             complement(2555..2983)
                     /locus_tag="AC16HB_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5605472.1"
                     /translation="MAMNVGPAEGEDEAISAINTTPLVDVMLVLLIIFLITIPVVTQQ
                     VPVQLPKETNVPRQTKPENIEIAVTKDGDVFWGLQQVKDNEELVARLKKIAVMQPQPE
                     VQIRGDLAGRYEFVGRVVFAAQRAGILKLGFVTEPPARGS"
     gene            complement(3004..3885)
                     /locus_tag="AC16HB_01405"
     CDS             complement(3004..3885)
                     /locus_tag="AC16HB_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5605473.1"
                     /translation="MFALIRHAALGLALVIAAATAPLTASAQDAAKSAAPAAAAPTPA
                     APAVDVPAVAKPAAPGVTPTFSKETIENPYGLEALWKQGDFVSKGTLIILVIMSMGSW
                     YIIFVKLWEQFKLMREGRAMQAGFWKAASLQDGTKKLKENSAYRFIAETGLEANDHHE
                     GSLTENIDLNTWVTMSIQRAVETVQNRLQDGLAFLATVGSTAPFVGLFGTVWGIYHAL
                     TAIGIAGQASIDKVAGPVGEALIMTAIGLAVAVPAVLGYNWLVRRNKVAMETVRTFGG
                     DLHAVLLGKRAETRAAR"
     gene            complement(3984..4637)
                     /locus_tag="AC16HB_01410"
     CDS             complement(3984..4637)
                     /locus_tag="AC16HB_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5605474.1"
                     /translation="MDFARQQRNPARHLIGIGGVILLHALVIYALLTGLARKVVEVVK
                     GPIEVKVIEEVIKKPPPPEVLPPPPKVAAPPPPFVPPPEIQIAPPPTPAPAITAVTQE
                     APPAPQAPVIQKPVETAPPAPPPPAIRSAAVVCPNYREVMGSITYPREALLDNIEGDV
                     VIEFTVAANGQIRDPIIKSSTNRVFNRVSLNVVQSRLQCQGQGQDVRVQAPISFKIK"
     gene            complement(4787..5131)
                     /locus_tag="AC16HB_01415"
     CDS             complement(4787..5131)
                     /locus_tag="AC16HB_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605475.1"
                     /translation="MNDSPTKRRKKTDNEADTLSAEAAHWLKELPESVRPMKTCARFP
                     RIVNGLVSVWDTPQRCRTYFDDLLLDRRGDRRGFPTDIAFELAALKNYFETAVHPSHQ
                     TIWDDIIQRQRS"
     gene            complement(5240..6271)
                     /gene="ruvB"
                     /locus_tag="AC16HB_01420"
     CDS             complement(5240..6271)
                     /gene="ruvB"
                     /locus_tag="AC16HB_01420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR5605476.1"
                     /translation="MVQPERIVAATPSSPNEEAVERALRPQRLVDYVGQTRIREQLDI
                     FVTAARNRGEALDHVLLFGPPGLGKTTLAHIVAHEMGVNLRQTSGPVLERPGDLAALL
                     TNLEKHDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGEGPAARSIKLDLPAFTLVG
                     ATTRAGMLTNPLRDRFGIVARLEFYSDEELTSIVLRSARLLNAVLDADGAREIARRSR
                     GTPRVANRLLRRVRDFAEVKAQGRITRAVADTALSMLDVDTRGLDVMDRKLLSAVIDK
                     FAGGPVGIENLAAAIGEERDTLEDVVEPFLIQQGMLQRTPRGRVATLNAYEHFGITAP
                     APARGPELF"
BASE COUNT          950 a         2048 c         2078 g         1196 t
ORIGIN      
        1 tccggcccgg cccggtgccg gactccatcg aaagctcgat ccccggccga ttcgagggtt
       61 gggtcggtaa cagccggttt cgtctgtcga acgggcaggt ctgggaaata cgtgacggtt
      121 cgcaggccag ctacgacctg caggacccaa aggttcgaat cgtgcgtggc ctgtccggaa
      181 cgttcttcat tgaaatcgcg ggtgtctcac agacgccgcg agttcgccgc gtcgagtgag
      241 ttcgaggtcg gtctgaatcg cggtcggcgc gggccggtac gaactctttt cctgcgcggc
      301 ggctcaaagc caggtcaagc cgctcgatgg tttggaggcc gcatgtcccg attgcccgtg
      361 cttttcttgt tgagtcttac ggcggttgca tccgttgccc gcgcagaagt gaatgtgagc
      421 ttcctcgcgc ccgagcggtt cactgacacg gcgcgcgacg gcggcttcgg ccggccagac
      481 aaaggcaaga ccttggacga gttcaagcga cacctggccg ggctcggcga gcgctgcatt
      541 ccgcctggac agtcactgga catcgtcatt cgcgacattg acctggctgg tcagcaagag
      601 ccgcggcgtg gagcggacgg ggaattcttg cgcgtcctga agggggtctc gaacccaagg
      661 atcgatctgg agtacgtctg gcggtcggcc gatgggaccg ttctggggaa gggtgcagag
      721 cgcgtcgccg aggcgagctt tcttccagaa cggactcatg atccgcgcac ccatgactcc
      781 ctgtactacg agaaggcgat gctcacccag tggttcgagg ctcgtttctg caagtccaca
      841 gaggccccca agaagtgagc ccgcggttcc gcccgttcgc tgtgccctag ggaagccgca
      901 tccacatccg cgccaggtcc gcggcgccgt cgctgccgcc gacggacttg aaggcctggg
      961 ccgccttgcc cttctgcccg gcacgcgtca gcgcgatgcc gaggtgaagc tgagcatcgt
     1021 tcggattgcg caggctggcc ttggcgatac ccttctggat cagttcgatg cccttgtcgt
     1081 actggcccag ccctgtgtac gcgagtccgg tccgcaccag cacgttgccg tcgcgcgcgg
     1141 ccatgccttc ggtctccgcc tccttcaggt cggcgggcgc ggctgcggcc ttctgtgttg
     1201 ccagcgccag caggcgcttc tggcgctcga cctcgccggt cttgccgagc aggcctgagg
     1261 caaaagcgtc gtcgaggact ttcttggctt cgcccgcctg acctgcctgc agcgcgagtt
     1321 gcgccatttc catcacgtcc tcggccttcg tcaggctgcc gatcgcgagc ttcagccgca
     1381 tcacgtccag cgcatgccgg tcggagaagc cgctccttcc gatcaggcgg ttcaggtaca
     1441 gcgcgaagta ctccttcttc gggtagtagc tgatgagctt ttcgatcgtc gctgcctgtc
     1501 cctgggtgtt accgagccgc atctgcgcat ccgccagacg cagcaagtca ccttcttggg
     1561 gtcgtccgcc ctgttcggcg gcactgatcg cagcctgggc gtcacgcgcg atggcgttgt
     1621 aatcgccgct ctgggcactc acgtagctca gcagctggtt gagctgcggg cttgatcccc
     1681 cggcctgctg tgccttggca gcccattcgc gcgtcttgtt ccagtccttg acctgggaga
     1741 aggcaaacgc gagctgctcc gcgacctgcg cctgctcgcc tgtgctcagc cggcccgatg
     1801 cataggcggc ctcgaaggag cggatcgcgg tggctggatc gccggcgcga aacgcggcgg
     1861 cgccgcgcat gcgatcgagc atcagttgct cggccggcgt cttgttcgga acgttctcgg
     1921 cctcgcgcac cttggccagc gcttccttgg cgcggccggc tttcagcagt tcggcggcct
     1981 gctgcaacgg cttgccgacc tctgggcgca gcccttgggc gtgcgcgatg ttcagagtca
     2041 gggccgctgc gaccgcgagc agcacgcgca cacagcgacc ggttcctttg tcgttcattt
     2101 cacgaactgc tcattgccga ccagaccgag cttggtcacg ccgagccgtt gcgccgcggc
     2161 catgacggcc gccacgtcct tgtattcggc gagcttgttc gggcgcagat ggacctcggg
     2221 ctgcggcacc gcggccgctg cgtcggtcag gcgcgcctcc agcgcatcct tgttcggcac
     2281 gatgtcgccg ttccagaaga tcgtgccgtc gaaatcgacg tcgatcgtga ccacgaccgg
     2341 tttttccagt ggcggcggcg ggttgccgac cggcatgttc aggttgaccg catgcaactg
     2401 gatcgggatc gtgatgatca gcatcaccag cagcaccagc atcacgtcga tcagcggcgt
     2461 ggtgttgatg tcgatcatca cctcgggctc gctcgaaccg acgccggggc cgacattcat
     2521 agccatttga atctccttgc gtgcgcgcgg gcgatcaact gccccgcgcc gggggttcgg
     2581 tcacgaagcc gagcttgagt atcccggcgc gctgcgcggc gaacacgacg cggccgacga
     2641 actcgtagcg gccggccaaa tcgccgcgga tctgcacttc cggctgcggc tgcatcaccg
     2701 caatcttctt caggcgggcg accagttcct cgttgtcctt cacctgctgc aggccccaga
     2761 acacgtcgcc gtccttggtg accgcgatct cgatgttctc gggcttggtc tgccgcggca
     2821 cattggtttc cttcggcagt tgcacgggaa cctgctgcgt gaccaccgga atcgtgatca
     2881 ggaagatgat cagcagcacc agcatcacgt cgaccagcgg cgtggtgttg atggcggaaa
     2941 tcgcctcgtc ctcgccttcg gcgggtccga cattcatcgc catggcggac tcctgtcagc
     3001 cggtcagcgc gcggcgcgcg tttcggcgcg cttgccgagc agcactgcgt gcaggtcgcc
     3061 tccgaaggtg cggaccgttt ccatggccac cttgttccgg cgcaccagcc agttgtagcc
     3121 gagcaccgcg ggcaccgcga ctgccaggcc gatcgccgtc atgatcagcg cctcgccgac
     3181 cgggccggcc accttgtcga tcgatgcctg accggcgatg ccgatcgccg tcagcgcgtg
     3241 atagatgccc cacacggttc cgaacagtcc cacgaacggc gcggtcgacc caactgtcgc
     3301 caggaaggcc aggccgtcct gcaggcggtt ctgaaccgtc tcgacggctc gctggatcga
     3361 catcgtgacc catgtgttca gatcgatgtt ctcggtcagg gatccctcgt gatgatcatt
     3421 ggcctccagc cccgtctcgg cgatgaaacg gtaggcgctg ttctccttga gtttcttggt
     3481 gccgtcctgc agcgaggccg ccttccagaa gccggcctgc atcgcgcgac cctcgcgcat
     3541 cagcttgaac tgctcccaca gcttcacgaa gatgatgtac cagctgccca tcgacatgat
     3601 cacgaggatg atcagcgtgc ccttggagac gaagtcgccc tgtttccaga gcgcttcgag
     3661 gccgtacgga ttctcgatcg tttccttgga gaacgtcggc gtcacgccgg gcgcagcagg
     3721 cttggccacg gccggcacgt cgaccgccgg cgcggcgggc gtgggcgctg cggcggcggg
     3781 cgcggcggac ttcgccgcgt cctgcgcgga tgcggtcagt ggagcggtcg cggccgcgat
     3841 cacgagcgcc agtccgaggg ccgcgtgtcg aatcagagca aacatgtgca agtctcctta
     3901 tggggaagtg ttgaagctcg ggcgccgggt cttgcgcctg tggcgtggcc ttttgggctg
     3961 cctcgatttc tctctttgcc gggttacttg atcttgaagc tgatgggtgc ctgaacgcgt
     4021 acgtcctgtc cctgcccctg acactgcagg cgtgactgga ccacgttcag cgatacgcga
     4081 ttgaagacgc gattcgtcga cgacttgatg atcggatcgc ggatctggcc gttcgcggcg
     4141 accgtgaact cgatgacgac gtcgccttcg atgttgtcga gcagcgcttc gcgcggatat
     4201 gtgatcgagc ccatcacctc gcggtagttc ggacacacga ccgccgcgct gcggatcgcc
     4261 ggcggtggcg gcgcgggcgg cgcggtttcg accggcttct ggatcaccgg cgcctgcggc
     4321 gccggcggcg cttcctgtgt caccgccgtg atcgctggcg ccggcgttgg cggcggggcg
     4381 atctggatct cgggcggcgg aacgaacggg ggcggcggcg cggcgacctt cggcggcggt
     4441 ggcagcacct cggggggcgg cggcttcttg atcacctctt cgatgacctt gacttcaatc
     4501 ggacccttga caacctcgac gaccttgcgc gcaagccccg tgagcagcgc gtagatgacg
     4561 agggcgtgca gcaggatgac tccgccgatg ccgatcagat gccgggccgg attgcgctgc
     4621 tggcgcgcaa aatccatggg gtgtcctcgt ggccgtgcca aatcgcaaag cgcgcgattc
     4681 tagtggttta cgcgcacttt ttccgctccg ggggagcggc tgatgtgatg cactgcagaa
     4741 gatcagcgtc gcggcgtggt cgtcgcgctg ccggagcggc gagtcctcag ctccgctgac
     4801 gctgaatgat gtcgtcccag atggtctggt gggacggatg caccgcagtc tcgaaatagt
     4861 tcttcagcgc cgcgagttca aaggcaatgt cggtcgggaa gccccggcga tcgccgcggc
     4921 ggtcgagcag caagtcgtcg aagtaggtgc ggcagcgctg cggcgtgtcc catacggaaa
     4981 cgaggccgtt cacgatgcgc ggaaagcgcg cgcaggtctt catcgggcgc acggactcgg
     5041 gcaactcttt cagccagtgc gcggcctcgg cgctcaaggt gtccgcctcg ttgtccgttt
     5101 tcttgcgccg ctttgtcggc gagtcgttca cggcagccct cccaggggtc tgtggtcgca
     5161 tgctcacgaa ttctggcggc atcccgcaga tgcgcgaatc atcctgccga cggatggggt
     5221 acgtaaccgg tcgctacggt cagaagagtt cgggcccccg cgccggtgcc ggcgcggtga
     5281 tgccgaagtg ctcgtaggcg ttcaacgtcg ctacgcgacc gcgcggggtg cgctgcagca
     5341 tgccctgctg gatcaggaac ggctcgacga cgtcttccag cgtgtcccgc tcctcgccga
     5401 tcgcggcggc gaggttttcg atcccgaccg gacctccggc gaacttgtcg atcaccgcgc
     5461 tcagcagctt gcggtccatc acatcgagcc cacgcgtgtc gacgtcgagc atcgacagcg
     5521 cggtgtcggc caccgcgcgc gtgatccggc cctgcgcctt cacctccgca aaatcgcgga
     5581 cgcggcgcag aagccggttg gcgacgcgcg gtgtgccgcg cgagcggcgc gcgatttcgc
     5641 gcgcgccgtc ggcatcgagc acggcgttca gcagccgcgc cgagcgcagc acgatcgaag
     5701 tcagctcctc gtcactgtag aactcaaggc gtgccacgat gccgaagcgg tcgcgcagcg
     5761 gattggtcag catgccggcg cgcgtggtcg cgccgaccag cgtgaacgcc ggcagatcga
     5821 gcttgatcga gcgagcggca gggccttcgc cgatcatgat gtcgatctgg aaatcctcca
     5881 gcgccgggta caggatctcc tcgaccacgg ggctcagccg atgaatctcg tcgatgaaca
     5941 gcacgtcgtg tttctcgaga ttcgtcagca gcgcggcaag atcgccgggc cgctcgagca
     6001 ccggcccgct ggtctggcgc aggttcacgc ccatctcgtg cgccacgatg tgtgccagcg
     6061 tcgtcttgcc cagtccaggc gggccgaaca gcagcacgtg atccagcgct tcgccgcggt
     6121 tgcgcgccgc ggtgacaaag atatcgagct gctcgcgaat ccgggtctgg ccgacgtagt
     6181 cgacaagccg ttgcgggcgc agcgcgcgct cgactgcttc ctcgttgggt gacgacggcg
     6241 tggcggcgac gatgcgttcc ggctgcacga tc
//
LOCUS       DBNHBY010000058         6489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_679700, whole genome shotgun sequence.
ACCESSION   DBNHBY010000058 DBNHBY010000000
VERSION     DBNHBY010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_679700"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC16HB_01425"
     CDS             <1..428
                     /locus_tag="AC16HB_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR5605477.1"
                     /translation="DAGLNSGFMIAHVTAAALASENKSLAHPASVDSLPTSANQEDHV
                     SMATFAARRLQPMIANTAHILGIELLAAAQGIEFLRPLRSSAALEQVHALLRERCPAM
                     AHDRFLAPDIEAATQLVTGGALSRVFRTLPELPALWIPS"
     gene            complement(448..1458)
                     /locus_tag="AC16HB_01430"
     CDS             complement(448..1458)
                     /locus_tag="AC16HB_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR5605478.1"
                     /translation="METAAIALPVVVLLVALAFAFDFMNGFHDAANSIATVVSTGVLK
                     PRQAVLLAAAFNVIALFVFQLHVAATIGKGIVEPDAVDHHVVFGALIGAITWNVVTWW
                     YGLPSSSSHALIGGIMGAVVAKAGVGKLIGAGIWKTVVFIFVSPTLGFVLGAMMMVAI
                     SWIFRRSAPLRVDRWFKRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIAAGLLAADAK
                     SPPAWVIIGCYLAIGLGTMFGGWRIVKTMGQRLTKLRPVGGFCAETGGALMLFTANVF
                     GIPVSTTHTITGSILGVGSVRNPSATRWGLAGNIVWAWIFTIPVSAFIAAIFYWVSRA
                     LL"
     gene            complement(1477..2124)
                     /locus_tag="AC16HB_01435"
     CDS             complement(1477..2124)
                     /locus_tag="AC16HB_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR5605479.1"
                     /translation="MVFGKLLPKEDNFFEMFNEHAHHIAAGARSFMSMIQHYGQQDLR
                     EKYSAEVVAAEHAADRITAEVNRKLHRTFVTPIDREQIHGLINAMDDILDLLQDSSEV
                     MALYNVQHLPADVERLGDISMKCCQRVKHAVSLLPELTRSQGAEAALKTCEEIDRLES
                     DADRVMRGAMSKLFRDDIDVRELIKLKAIYEHLESISDRCEDVANLIEGIVLEHS"
     gene            complement(2173..3561)
                     /locus_tag="AC16HB_01440"
     CDS             complement(2173..3561)
                     /locus_tag="AC16HB_01440"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIR5605480.1"
                     /translation="MAVSAFDLFKIGIGPSSSHTVGPMRAARMFALRLAHDGLLGRVV
                     RVRAELYGSLGATGKGHGSDKALILGLEGDEPDRVDIDGIEVRLAQVRTRQQLRLLSQ
                     FAIAFDPARDLLFYRTRTLPFHANGMRFVAFDAADAELLAKTYYSVGGGFVVSDEVAA
                     DGAALKRIAPDTTVLPHPFHTGDQLLAQTERARCSIAEIMRGNERHWKSDAEIDAALR
                     AIWDAMQACAARGLRAEGELPGPFKVKRRAPALYRKLSAPGAGLTDPLAVMDWVNLWA
                     LAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYTRFVPGASEARVFEFLLTAAAIGILY
                     KENASISGAEVGCQGEVGVACSMAAGALAAVLGGTPQQVENAAEIGMEHHLGLTCDPV
                     GGLVQIPCIERNAVASVKAINAARLALAGDGSHVVSLDNVIKTMRETGADMQTKYKET
                     ARGGLAVNIVEC"
     gene            complement(3570..4418)
                     /locus_tag="AC16HB_01445"
     CDS             complement(3570..4418)
                     /locus_tag="AC16HB_01445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5605481.1"
                     /translation="MTSARRRHLIGAALAAPLLAGCSVIEGMFFYPDRVQYTRPGDLG
                     LAHEDVSITAADGSGLHGWWLPANAAQGSVLHLHGNAANVSNHLPLVAWLPAAGLNVL
                     MLDYRGFGKSAGKPSLDGVVDDALAALRYLRARHDIDAGKLVVLGQSIGGATALRMLA
                     ADRTGVRLAVIDSAFASYRDIARDAALQSVVLAPFLPLALPLLPPRERDPIAMLDRID
                     LPLLFVHGTADAVIPFKHSERLFAAAREPKRLIRVDGGEHMEALMRSRVQADVRGAML
                     AAVSGT"
     gene            complement(4520..>6489)
                     /gene="gcvP"
                     /locus_tag="AC16HB_01450"
     CDS             complement(4520..>6489)
                     /gene="gcvP"
                     /locus_tag="AC16HB_01450"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR5605482.1"
                     /translation="PAYRLTLQTREQHIRREKATSNICTAQVLLAVMASMYAVYHGPH
                     GLKRIAQRVHRLATVFAAGLRASGLTTSERFFDTVTVTGVDAAKVHAAARAQRINLRD
                     LGPDAVGVSFDELSTRADVLTLWRLFGATGHVDVLDGAVRDAIPPARVRTSSFLTHPV
                     FNTHHAEHEMLRYLRSLADKDLALDRTMIPLGSCTMKLNATAEMIPVTWPQFANIHPF
                     APGEQAQGYRQLIEELEQMLVECTGYDAVSLQPNSGAQGEYAGLLAIRAYHKARGEGH
                     RNICLIPESAHGTNPASANLAGMDVVVVKCDANGNVDVADLKAKAAQHRAKLAALMVT
                     YPSTHGVFEEAIVEICETIHAHGGQVYTDGANLNALVGVAKPGKYGSDVSHLNLHKTF
                     CIPHGGGGPGVGPVAVKAHLAKFLPGGLGDVVNGEHPVGMVSAANHGSAGILPISWMY
                     VTLMGAAGLKRATQVALLAANYIARRLAPYYPTLYTGRNGLVAHECILDLRSIKDACG
                     ISAEDVAKRLMDYGFHAPTLSFPVPGTLMVEPTESEALHELDRFIDAMIAIRAEIRAV
                     EEGRVDKDDNPLRNAPHTAAMVMAENWEHDYTREQAAFPLATLKRQKYWPPVARIDNV
                     YGDRHVMCACPPVTAYREARPGESREVRSAA"
BASE COUNT          999 a         2279 c         2180 g         1031 t
ORIGIN      
        1 gggacgccgg cctgaactcc ggcttcatga ttgcgcacgt caccgcggcg gcgctggcca
       61 gcgagaacaa gagcctcgcg catccggcga gcgtcgattc gctgccgacc tcggccaacc
      121 aggaagacca cgtgtcgatg gccaccttcg cggcgcgacg cttgcagccg atgatcgcca
      181 acaccgcgca catcctcggc atcgaattgc tcgccgccgc gcagggcatc gagttcctgc
      241 gtccgctcag aagttcggcg gcgctcgagc aggtgcacgc gctgctgcgc gagcgctgcc
      301 cggcgatggc gcacgaccgg ttccttgcgc ccgatatcga agccgccacg caactggtca
      361 ccggcggcgc gctgtcgcgc gtgttccgca ccctgccgga gctgccggcg ttgtggattc
      421 cgtcgtaggt ggtccgggga ccgcgcgtca gagcagcgcg cggctgaccc agtagaagat
      481 agccgcgatg aacgcggaca ccgggatcgt gaagatccac gcccacacga tgttgccggc
      541 cagcccccag cgcgtcgccg acggattgcg caccgagccc acgccgagga tcgagccggt
      601 gatcgtgtgc gtggtcgaca ccggaatgcc gaacacgttg gccgtgaaca gcatcagcgc
      661 gccgccggtc tcggcgcaga aaccgccgac cgggcgcagc ttggtcagcc gctgtcccat
      721 cgtcttgacg atgcgccagc cgccgaacat cgtgccaagc ccgatcgcga gatagcagcc
      781 gatgatcacc cacgccggcg gcgacttggc gtccgccgcg agcaggccgg cggcgatcag
      841 aagcatccag atgatgccga tcgtcttctg cgcgtcgttg ccgccgtggc cgagcgagta
      901 caggctggcg gagacgagct gcagccgctt gaaccagcgg tccacgcgca gcggcgcgct
      961 gcgccggaag atccacgaga tcgccaccat catcatggcc cccagcacga accccagcgt
     1021 cggcgacacg aagatgaaca cgaccgtctt ccagatgccg gcgccgatca gcttgccgac
     1081 gcccgccttg gccaccaccg cgcccatgat gccgccgatc agcgcgtgcg aactcgacga
     1141 cggcagcccg taccaccagg tgacgacgtt ccaggtgatc gcgccgatca gcgcgccgaa
     1201 cacgacgtgg tgatcgaccg catccggctc gacgatgccc ttgccgatcg tcgccgccac
     1261 gtggagctgg aacacgaaga gggcaatcac gttgaacgcg gccgcgagca gcaccgcctg
     1321 gcgcggcttc agcacgccgg tggacacgac ggtcgcgatc gagttcgcgg cgtcgtgaaa
     1381 gccgttcatg aagtcgaagg cgaacgccag cgccaccagc agcacgacga ccgggagcgc
     1441 gatcgcagcc gtttccatcg cggatcccgg cgccggtcag gagtgctcga gcacgatgcc
     1501 ttcgatcagg ttcgccacgt cctcgcagcg atccgagatc gattccagat gctcgtagat
     1561 cgccttcagc ttgatcagct cgcgcacgtc gatgtcgtcg cggaacagct tcgacatcgc
     1621 gccgcgcatc acccggtccg cgtcggactc gagccggtcg atttcctcgc acgtcttcag
     1681 cgccgcttcc gcgccctggc tgcgcgtgag ttccggcagc agcgacaccg cgtgcttgac
     1741 gcgctggcag cacttcatcg agatgtcgcc cagccgctcg acgtcggcgg gcaggtgctg
     1801 cacgttgtac agcgccatca cctcgctgga gtcctgcagc aggtcgagga tgtcgtccat
     1861 cgcgttgatc aggccgtgga tctgctcgcg atcgatcggc gtgacgaacg tgcggtgcag
     1921 cttgcggttc acctcggccg tgatgcggtc ggcggcatgc tcggcggcga cgacctcggc
     1981 ggagtacttc tcgcgcaggt cctgctggcc gtaatgctgg atcatcgaca tgaacgagcg
     2041 cgcacccgcg gcaatgtggt gggcgtgctc gttgaacatc tcgaagaagt tgtcttcctt
     2101 cggtagcagc ttgccgaaca cgattgcgcg ctccccgtgg ttatcgggtc cgattgtagg
     2161 caagtgtgcg actcagcact cgacgatgtt caccgccaat cccccgcgcg cggtttcctt
     2221 gtacttggtc tgcatgtcgg cgccggtctc gcgcatcgtc ttgatcacgt tgtcgagcga
     2281 caccacgtgc gagccgtcgc ccgcgagcgc caggcgcgcg gcgttgatcg ccttcacgct
     2341 cgccacggcg ttcctctcga tgcacgggat ctgcaccagc ccgccgaccg gatcgcaggt
     2401 gagcccgagg tggtgctcca tgccgatctc ggccgcgttc tcgacctgct gcggcgtgcc
     2461 gccgagcacc gcggccagcg cacccgcggc catcgagcag gcgacaccga cctcgccctg
     2521 gcaaccgacc tcggcgccgc tgatcgacgc gttctccttg tacaggatgc cgatcgcggc
     2581 cgcggtcagc aggaattcga atacgcgcgc ctcggatgcg cccggcacga agcgcgtgta
     2641 gtaatgcagc accgccggga tgatgccggc cgcgccgttc gtgggcgcgg tgaccacgcg
     2701 gccgccggcg gcgttctcct cgttgaccgc gagcgcccac aggttgaccc agtccatcac
     2761 cgcgagcgga tcggtgagtc cggcgccggg cgcggacagc ttgcggtaca gcgccggcgc
     2821 gcgtcgcttc accttgaatg gtcccggcag ttcgccttcg gcccgcagtc cgcgcgcggc
     2881 gcacgcctgc atcgcgtccc agatcgcgcg cagggcggcg tcgatctcgg cgtcgctctt
     2941 ccagtggcgt tcgttgccgc gcatgatctc ggcaatcgag cagcgcgcgc gttcggtctg
     3001 cgccagcaac tgatcgccgg tgtggaacgg atgcggcagc accgtggtgt cgggcgcgat
     3061 gcgcttgagc gcggctccgt cggccgccac ctcgtcgctg acgacgaagc cgccgccgac
     3121 cgagtagtag gtcttcgcga gcagttcggc gtccgcagcg tcgaacgcga cgaaccgcat
     3181 gccgttggcg tgaaacggaa gcgtccgggt ccgatagaag agcagatcgc gcgccggatc
     3241 gaacgcgatg gcgaactggc tgagcaatcg caactgctgc cgagttcgca cctgcgccag
     3301 gcgcacttcg atgccgtcga tgtcgacccg gtccggttcg tcgccctcga gcccgaggat
     3361 cagcgccttg tccgaaccgt gacccttgcc ggtcgctccg agcgagccgt agagctccgc
     3421 ccggacgcgg accacccgcc cgagcagtcc gtcgtgcgcc agccgcagcg cgaacatgcg
     3481 cgccgcgcgc atcggaccga cggtgtgcga cgacgacggc ccgatgccga tcttgaacag
     3541 gtcgaaggcg ctgacggcca tgcgacgtcc tacgtaccgg aaaccgcggc gagcatcgca
     3601 ccgcgcacat ccgcctgcac gcggctgcgc atcaacgcct ccatgtgctc gccgccatcg
     3661 acgcggatca gccgcttcgg ttcgcgcgcg gcggcgaaga ggcgctcgct gtgcttgaat
     3721 ggaatcaccg catcggcggt gccgtgaacg aagagcagcg gcaggtcgat gcgatcgagc
     3781 atcgcgatgg gatcgcgctc gcgtggcggc agaagcggca gcgcgagcgg caggaacggg
     3841 gcgagcacga cgctttgcag cgccgcgtcg cgcgcgatgt cgcggtagct cgcgaatgca
     3901 gagtcgatca ccgccagcct cacgccggtg cgatcggccg ccagcatgcg cagcgcggtc
     3961 gcgccgccga tgctctggcc gagcacgacc agcttgcccg catcgatgtc gtgccgcgcg
     4021 cgcagatagc gcagcgcggc cagcgcatcg tcgaccacgc cgtcgagcga tggcttgcct
     4081 gcggacttcc cgaagccacg gtagtcgagc atcagcacgt tcaggcccgc cgcgggcagc
     4141 caggccacca gcggcagatg gttgctgacg ttggccgcgt tgccgtgcag atgcagcacg
     4201 ctgccctgcg ccgcgttggc cggcagccac cacccgtgca gcccgctgcc gtcggcggcc
     4261 gtgatcgaca catcctcgtg cgcgagcccg aggtcgcccg gccgcgtgta ctgcacgcgg
     4321 tccgggtaga agaacatgcc ttcgatcacg ctgcaccccg ccagcagcgg cgcggcgagt
     4381 gcggcgccga tcagatgccg gcgccgcgcg gaagtcacgc cgcggccggc gcctctttcc
     4441 tgtgcgcgtc gatcggcacg cacgagcagg acacgtggcg gtcgccgtac acgttgtcga
     4501 tgcgcgcccg cacagccggt caggctgcgc tcctcacctc gcggctttcg ccggggcgcg
     4561 cttcccggta ggccgtgacg ggaggacacg cgcacatcac atggcgatcg ccgtagacgt
     4621 tgtcgatgcg tgccaccggc ggccagtact tctgccgctt cagtgtcgcc agcgggaacg
     4681 cggcctgttc gcgcgtgtag tcgtgttccc agttctcggc catcaccatc gcggcggtgt
     4741 gcggcgcgtt gcgcagcggg ttgtcgtcct tgtcgacgcg cccctcctcg accgcgcgga
     4801 tctcggcacg gatcgcgatc atcgcgtcga tgaagcggtc cagttcgtgc agcgcctcgc
     4861 tttcggtcgg ctcgaccatc agcgtgccgg gcaccggaaa actgagcgtc ggcgcgtgga
     4921 aaccgtaatc catcagccgc ttcgccacgt cctcggcgct gatgccgcag gcgtccttga
     4981 tcgaacgcag atcgagaatg cactcgtgcg cgaccagccc gttgcggccc gtgtacagcg
     5041 tcgggtagta gggcgcaagg cgtctggcga tgtagttcgc ggcgagcagc gcaacctgcg
     5101 tggcgcgctt caacccggcc gcgcccatca gcgtgacgta catccacgag atcggcagga
     5161 tgccggccga gccgtggttt gcagcgctga ccatgccgac cgggtgttcg ccgttgacga
     5221 cgtcgccgag cccgcctggc aggaacttcg ccaggtgcgc cttgaccgcg accggtccga
     5281 cgcccgggcc cccgccgccg tgcggaatgc agaacgtctt gtgcaggttc aggtgcgaca
     5341 cgtccgagcc gtacttgccc ggcttggcca cgccgaccag cgcattgagg ttcgcgccgt
     5401 ccgtgtagac ctgcccgccg tgcgcatgga tggtctcgca gatctcgacg atcgcctcct
     5461 cgaacacgcc gtgggtcgac gggtaggtga ccatcagcgc ggcgagcttg gcgcggtgct
     5521 gcgcggcctt ggctttcagg tccgcgacat cgacgttgcc gttggcgtcg cacttcacca
     5581 ccacgacgtc catgccggcg aggttcgcgc tcgcgggatt ggtgccgtgc gcgctctcgg
     5641 gaatcaggca gatgttgcgg tggccctcgc cgcgtgcctt gtggtacgcg cggatcgcga
     5701 gcagccccgc gtactcgccc tgcgcacccg agttcggctg caggctgacc gcgtcgtagc
     5761 cggtgcactc gaccagcatc tgctccagtt cctcgatcag ctgccggtag ccctgcgcct
     5821 gctcgcccgg tgcgaacgga tggatgttcg cgaactgcgg ccacgtgacc gggatcatct
     5881 ccgcggtggc attgagcttc atcgtgcacg agccgagcgg gatcatcgtg cggtcgagcg
     5941 cgagatcctt gtccgccagc gagcgcagat agcgcagcat ctcgtgctcg gcgtgatgcg
     6001 tgttgaacac cgggtgcgtc aggaacgacg acgtgcgcac ccgcgccggc gggatcgcgt
     6061 cgcgcaccgc gccgtcgagc acgtcgacat ggccggtggc gccgaacagc cgccacagcg
     6121 tcagcacgtc ggcgcgcgtg ctcagctcgt cgaacgagac gccgactgca tccggaccga
     6181 ggtcacgcag gttgatgcgc tgcgcgcgcg cggccgcatg caccttggcc gcatcgacgc
     6241 cggtcaccgt cacggtgtcg aagaagcgct cgctcgtcgt caggccgctc gcgcgcaggc
     6301 cggcggcgaa gaccgtcgcc agccggtgca cgcgctgcgc gatgcgcttc agtccgtgcg
     6361 gcccgtgata gacggcgtac atgctcgcca tcaccgccag cagcacctgc gcggtgcaga
     6421 tgttcgaggt cgccttctcg cgccggatgt gctgctcgcg cgtctgcagc gtgaggcgat
     6481 acgcgggct
//
LOCUS       DBNHBY010000059         2641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1627151, whole genome shotgun sequence.
ACCESSION   DBNHBY010000059 DBNHBY010000000
VERSION     DBNHBY010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1627151"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1199
                     /gene="hemW"
                     /locus_tag="AC16HB_01455"
     CDS             9..1199
                     /gene="hemW"
                     /locus_tag="AC16HB_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR5605483.1"
                     /translation="MSAYLRPGTLHLPALPPLALYVHVPWCLRKCPYCDFNSHERHGE
                     IPEDDYLDALRLDLECSMPLIWGRRIVSVFIGGGTPSLLSARAIDRLLSEVRALLPLE
                     PDCEVTLEANPGTFEADKFASFRASGINRLSIGIQSFSDAKLAALGRVHDAAQARAAI
                     EIAHRHFDNFNFDLMYALPAQTPEELEQDIGQALCAAPPHLSLYQLTLEPNTVFAKYP
                     PPLPDEDTAAAMHEGIEAATAAAGYSHYEVSAYARAERTCRHNLNYWSFGDYLGIGAG
                     AASKLSFPHRIVRQTRLRQPDSYLRAAARREFIAESHEVARADLPFEFMLNALRLNAG
                     FPLRWFTERTGLPLSAIDAAVGVAEQRGLLERDLDSIRPTLLGRRFLSDLQSLFLPRP
                     APVR"
     gene            1440..>2641
                     /gene="glnA"
                     /locus_tag="AC16HB_01460"
     CDS             1440..>2641
                     /gene="glnA"
                     /locus_tag="AC16HB_01460"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR5605484.1"
                     /translation="MASAKEVMKMITDNEVKFVDLRFTDFRGKEQHVSVPVKVFDDDK
                     FEHGHAFDGSSIAGWKGIEASDMLLMPDPATARMDPFREENTLILSCDVIEPSTGKGY
                     ERDPRSLARRTEAYLKSTGLGDTAYFGPEPEFFIFDSVTWNVDMSGCSVKIKSEEAPW
                     STGIDYEAGNLAHRPAVKGGYFPVPPTDSLQDIRSEMCLLLEQQGVEVEVHHHEVAAP
                     GQCEIGTKFATLVKRADWMQILKYTIWNVAASYGKTATFMPKPVVGDNGSGMHVHQSV
                     WKDGVNLFAGNGYAGLSDFALYYICGIIKHSKALNAFTNPGTNSYKRLVPGFEAPINL
                     AYSARNRSAACRIPYVSNPKGRRVEVRFPDPTANPYLAFAAMLMAGLDGVQNKIHPGE
                     PMDKNLYDL"
BASE COUNT          463 a          878 c          854 g          446 t
ORIGIN      
        1 cggccgatgt gtctgcgtat ctgcgtcccg gcacgctgca cctgccggcc ttgccgccgc
       61 tcgcgctgta cgtccacgtg ccctggtgcc tgcgcaagtg cccgtactgc gacttcaact
      121 cgcacgagcg gcatggcgag atcccggaag acgactatct cgacgcgctg cgcctcgacc
      181 tcgaatgttc gatgccgctg atctggggcc ggcggatcgt gagcgtgttc atcggcggcg
      241 gcacgccaag cctgctgtcg gcacgggcga tcgatcgttt gctgtcggag gtgcgggcgc
      301 tgctgcccct ggagccggat tgcgaggtca cgctcgaagc caaccccggc acattcgagg
      361 ccgacaagtt cgcatcattc cgtgcgtcgg gcatcaaccg gctttcgatc ggcatccagt
      421 cgttcagtga cgccaagctc gccgcgcttg gtcgtgtgca cgatgcggcg caggcgcgcg
      481 cggcgatcga gatcgcgcac cgccacttcg acaacttcaa tttcgacctg atgtacgcgc
      541 tgccggcgca gacaccggag gaactggagc aggacatcgg ccaggcactg tgcgcagcac
      601 caccacacct gtcgctgtac cagttgacgc tcgagccgaa caccgtattc gccaagtacc
      661 cgccgccgct gcctgatgag gacacggctg ccgcgatgca cgaagggatc gaagcggcga
      721 ccgccgcggc gggttattcg cactacgagg tgtccgccta cgcgcgggcc gagcgcacgt
      781 gccggcacaa cctcaactac tggagcttcg gcgattacct tggcatcggc gccggcgcgg
      841 ccagcaagct gtcgttcccg caccggatcg tgcggcagac ccgtctcagg cagcccgaca
      901 gctatcttcg cgcggcggcg cgacgcgaat tcatcgccga gtcgcatgag gtcgcgcgcg
      961 ccgacctgcc gttcgaattc atgctcaatg cgctgcggct gaacgcgggc tttccgctgc
     1021 gctggttcac tgagcgcacg ggactgccgc tgtctgcgat cgacgccgcg gtcggcgttg
     1081 ccgagcagcg cgggctgctc gagcgcgacc tcgattcgat ccggccgacg ctcctcggcc
     1141 ggcgcttcct cagcgacctg cagtcgcttt tcctgccgcg ccctgctccg gtgcggtagg
     1201 cgttccatag gtgcataatg caccttgata gtgcatatgg tccgagcgga ggccgggacg
     1261 tgggtctgcg gcaggcaccg ctaaatccct gacgcacgag gccggtgcgt cgaagcgcgg
     1321 cgatggcgca gcgcggcacg cttcctgcta agcatcgaat cgagcgccgg atttcggcgt
     1381 cacgacacca tcggcccggc cgaacacacg aaccgccctt tcgaagagga gaacactcga
     1441 tggcgagcgc caaagaagtc atgaagatga tcaccgacaa cgaagtcaaa ttcgttgacc
     1501 tgcgctttac cgatttccgg ggcaaggagc agcacgtctc ggtgcccgtc aaggtgttcg
     1561 acgacgacaa gttcgagcac ggccacgcgt tcgacggctc gtcgatcgcc ggctggaagg
     1621 gcatcgaggc gtccgacatg ctgctgatgc cggatccggc caccgcgcgg atggacccct
     1681 tccgcgagga aaacacgctg atcctgtctt gcgacgtgat cgagccgtcg accggcaagg
     1741 gctacgagcg cgatccgcgc tcgcttgcgc gccgcaccga ggcgtacctg aagtcgacgg
     1801 gcttgggcga caccgcctac ttcggtcccg agcccgagtt cttcatcttc gacagcgtga
     1861 cgtggaacgt cgacatgtcg ggctgctccg tgaagatcaa gtcggaggaa gcgccgtggt
     1921 cgaccgggat cgactatgaa gcgggcaacc tcgcgcatcg cccggccgtc aagggcggct
     1981 actttccggt gccgccgacc gactcgctgc aggacatccg ctccgagatg tgcctgctgc
     2041 tcgagcagca gggcgtggag gtcgaggtgc accaccacga agtggcggcg cccggccagt
     2101 gcgagatcgg caccaagttc gcgacgctgg tcaagcgcgc cgactggatg caaatcctca
     2161 agtacaccat ctggaacgtc gcggcgagct acggcaagac ggcgaccttc atgccgaagc
     2221 cggtcgttgg cgacaacggc tcgggcatgc acgtgcacca gtcggtatgg aaggacggcg
     2281 tgaacctctt cgccggcaac ggctacgcgg ggctgtccga ctttgcgctg tactatatct
     2341 gcggcatcat caagcattcc aaggcgctca atgcgttcac caatccgggc accaacagct
     2401 acaagcggct ggtgccgggc ttcgaggcgc cgatcaacct ggcgtattcg gcgcgcaacc
     2461 gctcggcggc ctgccgcatc ccgtacgtgt cgaacccgaa gggccgtcgc gtcgaagtgc
     2521 gcttcccgga tccgaccgcc aatccgtatc tggcgttcgc ggcgatgctg atggcgggtc
     2581 tggacggcgt gcagaacaag atccacccgg gcgagccgat ggacaagaac ctgtacgacc
     2641 t
//
LOCUS       DBNHBY010000060         6753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_476709, whole genome shotgun sequence.
ACCESSION   DBNHBY010000060 DBNHBY010000000
VERSION     DBNHBY010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_476709"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="secY"
                     /locus_tag="AC16HB_01465"
     CDS             complement(<1..1030)
                     /gene="secY"
                     /locus_tag="AC16HB_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5605485.1"
                     /translation="MANVPGTLGKTGKYGDLKRRLLFLVLALLVYRVGTHIPVPGIDP
                     DTLRQLFNSQQGGILGLFNMFSGGALSRFSVFALGIMPYISASIIMQMLTYVLPTLEA
                     LRKEGESGRRRITQYTRYGTLGLAIFQAASISIALESQQNLVLDPGLMFRFTCVVSLV
                     TGTIFLMWLGEQITERGLGNGISILIFAGIVAGLPNAIGGLFELVRTGAIGPLSAIVI
                     IGLIVLVTAFVVFVERGQRKILVNYAKRQVGNKIYGGQSSHLPLKLNMSGVIPPIFAS
                     SLILFPATLASWFTTGDATRWIRDLAAALSPGQPLYILLYASLIIFFCFFYTALVFSS
                     KETADNLKK"
     gene            complement(1241..1666)
                     /gene="rplO"
                     /locus_tag="AC16HB_01470"
                     /pseudo
     CDS             complement(1241..1666)
                     /gene="rplO"
                     /locus_tag="AC16HB_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(1685..1864)
                     /gene="rpmD"
                     /locus_tag="AC16HB_01475"
     CDS             complement(1685..1864)
                     /gene="rpmD"
                     /locus_tag="AC16HB_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5605486.1"
                     /translation="MSKKTLKVTLVKSPVSAKKDHQATVLGLGLKRMHQTRELEDTPA
                     VRGMINKVAYMVRVA"
     gene            complement(1876..2406)
                     /gene="rpsE"
                     /locus_tag="AC16HB_01480"
     CDS             complement(1876..2406)
                     /gene="rpsE"
                     /locus_tag="AC16HB_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR5605487.1"
                     /translation="MAKVQPRGMKGVASEERDDGLREKMIAVNRVTKVVKGGRILGFA
                     SLTVVGDGDGRIGMGKGKAREVPVSVQKAMEKARRNLKKAPLKDGTLHHAVLGRHGAS
                     RVFLAPAQQGAGVIAGGPLRAIFDVMGIKNIVAKSHGSTNPYNMVRATLNALEQLRTP
                     AEIAAKRGKTVEELLG"
     gene            complement(2417..2782)
                     /gene="rplR"
                     /locus_tag="AC16HB_01485"
     CDS             complement(2417..2782)
                     /gene="rplR"
                     /locus_tag="AC16HB_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR5605488.1"
                     /translation="MDKKQTRLRRARATRVRIALQRSHRLTVYRSNLHIYASVISPDG
                     GKVLVTASTAEPEVRSQLAGPKGKGGNVAAAKLVGTRLAQKAKAAGIETVAFDRAGFR
                     FHGRVKALADAAREAGLKF"
     gene            complement(2806..3339)
                     /gene="rplF"
                     /locus_tag="AC16HB_01490"
     CDS             complement(2806..3339)
                     /gene="rplF"
                     /locus_tag="AC16HB_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5605489.1"
                     /translation="MSRVARIPVNLGKGVEAQVSAGEITVKGPLGTLKHPVSPRVTVA
                     VTDGKVSFAATDASREANAMSGTLRALVAAMVQGVSTGFQKKLTLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHQMPAGIKCETPTQTEIVIKGADKQKVGQVAAEVRGYKPPEPYKGK
                     GVRYADETVVLKETKKK"
     gene            complement(3359..3754)
                     /gene="rpsH"
                     /locus_tag="AC16HB_01495"
     CDS             complement(3359..3754)
                     /gene="rpsH"
                     /locus_tag="AC16HB_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5605490.1"
                     /translation="MSMSDPIADMLTRIRNAQRIEKTEVVMPSSKLKVAIAQVLKDEG
                     YIEGFQVAANDGKPELHIGLKYYAGRPVIEQLERVSRPGLRVYKGRNAIPQVMNGLGV
                     AIVSTPQGVMTDRKARATGVGGEVLCYVA"
     gene            complement(3770..4075)
                     /gene="rpsN"
                     /locus_tag="AC16HB_01500"
     CDS             complement(3770..4075)
                     /gene="rpsN"
                     /locus_tag="AC16HB_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR5605491.1"
                     /translation="MAKLALINRQAKREKLVKKYEARRKDLKAALADPTRSMEERMEA
                     RAKLQALPRNASPTRLRNRCALTGRPRGTFRKFGLGRAKIREAAMRGDIPGIIKASW"
     gene            complement(4098..4637)
                     /gene="rplE"
                     /locus_tag="AC16HB_01505"
     CDS             complement(4098..4637)
                     /gene="rplE"
                     /locus_tag="AC16HB_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5605492.1"
                     /translation="MARLQTLYREKIVPELIQKFGYTSTMQVPRITKVTLNMGVGEAV
                     NDKKNIDMSVADLTKIAGQKPVVTKARKAIANFKIRQGLAIGCMVTLRGQRMYEFLDR
                     LVTIAFPRVRDFRGVSGRAFDGQGNYNIGLKEQIIFPEIEYDKIDKLRGMNISITTTA
                     KSDEEAKALLAAFRFPFRN"
     gene            complement(4647..4967)
                     /gene="rplX"
                     /locus_tag="AC16HB_01510"
     CDS             complement(4647..4967)
                     /gene="rplX"
                     /locus_tag="AC16HB_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR5605493.1"
                     /translation="MDRIRKGDEVVVIAGRDKGKRGVVLARIDERHVTVEGVNVVKKH
                     VRPNPMKGVTGGIVDKTLPIDVSNVMLFNPATKKGARVGFKVVDGKKVRVFKGGGTVV
                     GAKA"
     gene            complement(4979..5347)
                     /gene="rplN"
                     /locus_tag="AC16HB_01515"
     CDS             complement(4979..5347)
                     /gene="rplN"
                     /locus_tag="AC16HB_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5605494.1"
                     /translation="MIQMQTMLDVADNTGARSVMCIKVLGGSKRRYAGIGDVIKVTVK
                     DAAPRGRVKKGEVYSAVVVRTAHGLRRPDGSSIKFDSNAAVLLNPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(5531..5797)
                     /gene="rpsQ"
                     /locus_tag="AC16HB_01520"
     CDS             complement(5531..5797)
                     /gene="rpsQ"
                     /locus_tag="AC16HB_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR5605495.1"
                     /translation="MTTEAKTKRTLVGRVVSNKMDRTVTVLVERRVKHPMYGKYVVRS
                     TKFHAHDASNQVNEGDLVEIAETRPISRTKSWMVTRVVEAATTV"
     gene            complement(5794..5988)
                     /gene="rpmC"
                     /locus_tag="AC16HB_01525"
     CDS             complement(5794..5988)
                     /gene="rpmC"
                     /locus_tag="AC16HB_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5605496.1"
                     /translation="METKELRGKDAAALSKELNDLLRAHFSLRMQKATQQLQNTSQLR
                     RVKRDIARVRTVMAEKAAAK"
     gene            complement(6003..6419)
                     /gene="rplP"
                     /locus_tag="AC16HB_01530"
     CDS             complement(6003..6419)
                     /gene="rplP"
                     /locus_tag="AC16HB_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5605497.1"
                     /translation="MLQPARRKYRKDHKGRNRGLATRGANVAFGEFGLKATERGRLTA
                     RQIEAARRAISRHIKRGGRVWIRIFPDKPISQKPAEVRMGNGKGNPEYWIAQIQPGKV
                     IYEMDGVDESVAREAFALAAAKLPIKTTFVIRQLGQ"
     gene            complement(6422..>6753)
                     /locus_tag="AC16HB_01535"
     CDS             complement(6422..>6753)
                     /locus_tag="AC16HB_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5605498.1"
                     /translation="GFSEAKTTYGVIGVKVWVYKGDTLGRNEAPAVATEPTPAPERED
                     RRPAARRPAGKRPARRTEAGKPEGEATPAPAKEEAAPKPAVKRVRKAAAKSASADAKT
                     GGGKSGE"
BASE COUNT         1006 a         2369 c         2030 g         1348 t
ORIGIN      
        1 tcttcttcag gttgtccgcc gtttccttgc tgctgaacac gagcgccgtg tagaagaagc
       61 agaagaagat gatcaggctc gcgtaaagca ggatgtacag cggctgcccc ggcgacagcg
      121 ccgcggccag gtcgcggatc cagcgagtcg cgtcgcccgt cgtgaaccag ctcgccagcg
      181 tggccgggaa caggatcaga gacgacgcga agatcggcgg gatcacgccg gacatgttca
      241 gcttcagcgg cagatgcgag ctctggccgc cataaatctt gttgccgacc tggcgcttgg
      301 cgtaattgac caggatcttg cgctggccgc gctcgacgaa caccacgaag gccgtgacca
      361 gcacgatcag cccgatgatc acgatcgccg acagcggccc gatcgcgccg gtgcgcacca
      421 gttcgaacaa cccgccgatc gcgttcggca gcccggcgac gatgccggca aatatcagga
      481 tcgagatgcc gttacccagc ccgcgctcgg tgatctgctc gccgagccac atcaggaaga
      541 tcgtgccggt caccagcgaa accacacagg tgaagcggaa catcaggccc ggatcgagca
      601 cgaggttctg ctgcgattcg agcgcaatcg agatgctggc ggcctggaag atcgccagcc
      661 ccagcgtgcc gtagcgcgtg tactgcgtga tcctgcgccg gcccgactcg ccttccttgc
      721 gcagcgcctc cagcgtcggc agcacgtagg tgagcatctg catgatgatc gacgccgata
      781 tgtacggcat gatcccgagc gcgaacaccg agaagcgcga cagcgccccg ccggagaaca
      841 tgttgaacag cccgaggatg ccgccctgct gcgagttgaa cagttgccgc agcgtgtcgg
      901 gatcgatccc cggcaccgga atgtgcgtgc cgacccgata gacgagcagc gccagcacca
      961 ggaacagcag gcgccgcttc aggtcgccgt acttgcctgt cttgccgagg gtgccaggaa
     1021 cgttggccac gcgaatcctt gagtccagtt acttcgccgc gggcttggcg gatttcttcg
     1081 gcgccgtgtc cgacgcaggc ttggccgcct tcttcgccgc ctccggcttc ttctcggccg
     1141 gcttctgtgt cgctgccgca atcgacgccg cgagcgcttt gcgccgctcg gcgaagcgcg
     1201 cttccttctt cgcgccggcc ttcctagctt cggcgacgac gacggttccg ccagcggcct
     1261 cgatcgccgc cttggcgccc ttggtcacgc cgatgccgcg cacgctgatc ttgcggccga
     1321 tctcacccga cttgatcact cgtgcatcca gtgccaatgg cgagatcaca cccgcttgct
     1381 tcagtaccat caggtcgatc tcgtcagcgc cgagcttgtg caactcggac agccgtaccg
     1441 cctcgacgaa gcgccgcgac cgtgaagtga agccgcgctt gggcaggcgg cgatgcagcg
     1501 gcatctgccc gccctcgaag ccgaccttgt ggaatccgcc ggcgcgcgcc ttctggccct
     1561 tgtggccgcg gcccgccgtc ttgccccacc ccgagccgat gccgcggccg acgcgatgcg
     1621 cggcgctctt ggctcccgcg gcgggtttca gatcattcag tcgcatgacg aaagctcccg
     1681 gtccctaagc gacgcgcacc atgtacgcga ccttgttgat cattccgcgc accgctggcg
     1741 tgtcctccag ttcgcgcgtc tggtgcatcc tcttcaggcc gagcccgagc acggtggcct
     1801 ggtgatcctt cttcgccgac accgggcttt tgaccagcgt gactttcaaa gtcttcttcg
     1861 acatctgatc ctcgctcagc cgagcagttc ctcgaccgtc ttgccgcgct tggcggcgat
     1921 ttccgccggc gtgcgcagtt gctcgagtgc attcagcgtc gcgcgcacca tgttgtacgg
     1981 attggtcgag ccgtgcgact tggcaacgat gttcttgatg cccatcacgt cgaagatcgc
     2041 gcgcaacggg ccccccgcga tcacgcccgc gccctgctgc gccggcgcca ggaacacgcg
     2101 cgatgcgccg tgccgtccca gcaccgcgtg atgcagcgtg ccgtccttca gcggagcctt
     2161 cttcagatta cgacgtgcct tttccatcgc cttctgcacc gacaccggca cttcgcgagc
     2221 cttgcccttg cccatgccga tgcggccgtc gccgtcgccg accacggtca gcgacgcgaa
     2281 gccgaggatg cggccgccct tcacgacctt ggtgacccgg ttcaccgcga tcatcttttc
     2341 gcgcaagccg tcgtcgcgct cttccgacgc cacgcccttc atgcctctgg gttgcacctt
     2401 tgccatcgcc tgtccctcag aatttcaagc cggcttcgcg cgcagcgtcg gccagcgctt
     2461 tcacgcggcc atgaaagcgg aatcccgcgc gatcgaacgc gacggtctcg atgccagcag
     2521 ccttggcctt ctgcgccaag cgcgtgccga cgagcttcgc ggcagcgacg ttgccgccct
     2581 tgcctttcgg gccggccagc tgcgaacgca cttcgggctc ggcggtcgac gcggtcacca
     2641 gcaccttgcc gccgtccggc gagatcaccg acgcgtagat gtgcaggttc gagcggtaca
     2701 cggtcagccg atgcgaccgc tgcagcgcaa tgcgcacgcg cgtcgcacgc gcacggcgca
     2761 ggcgggtttg cttcttgtcg atcatgatgt gactccgtcc gcgcgctatt tcttcttggt
     2821 ctctttcaag accacggtct cgtcggcgta ccgcacgccc ttgcccttgt agggttccgg
     2881 cggcttgtat ccgcgcactt cggcggcgac ctgtccaacc ttctgcttgt cggcgccttt
     2941 gatgacaatc tcggtctgcg tcggagtctc gcatttgatg cccgcaggca tctgatgcac
     3001 gaccggatgc gagaacccga gcgacaggtt cagcttgtcg ccctgcgcct gcgcgcgata
     3061 accaacgccg accagcgtca gcttcttctg gaaacccgtc gacacgccct gcaccatggc
     3121 ggcgacgagc gcacgcagcg tgccgctcat cgcgttggct tcgcgcgatg cgtcggtggc
     3181 cgcaaagctg accttgccgt cggtgaccgc aaccgtcacc cgcggcgaaa ccggatgctt
     3241 cagcgtgccg aggggccctt ttaccgtgat ttcgccggcg ctcacctggg cttcgacgcc
     3301 cttgccgaga ttcaccggga ttcgtgcaac tcgagacatg gttcctctcc ccgcgcggtc
     3361 aggcgacgta gcacaggact tcgccgccga cgccggtggc gcgcgccttg cgatcggtca
     3421 tcacaccctg cggcgtcgag acgatcgcca cacccagccc gttcatcacc tgcgggatcg
     3481 cgttgcgccc cttgtagacg cgcagccccg gacgcgacac gcgctctagc tgctcgatca
     3541 cgggacggcc ggcgtagtac ttcagcccga tgtgcaattc cggtttgccg tcattggcag
     3601 cgacctggaa gccctcgata tagccctcgt ccttcaggac ctgagcgatc gcaactttca
     3661 gcttcgacga gggcatcacc acctcggtct tctcgatccg ctgggcgttg cggatgcggg
     3721 tcagcatgtc cgcgatcgga tcactcatgc tcatgtgcaa tctcctcgct taccagctgg
     3781 ccttgatgat gccgggaatg tcgccgcgca tcgccgcttc gcggatcttg gcgcgaccca
     3841 gtccgaactt gcggaacgtg ccgcgcgggc ggccggtcaa cgcgcagcgg ttgcgcagcc
     3901 gcgtcgggct ggcgttgcgc ggcagcgcct gcagcttcgc gcgcgcctcc atccgctcct
     3961 ccatcgagcg cgtcggatcc gcgagcgcgg ccttcaagtc cttgcgcctg gcctcgtact
     4021 tcttgacgag cttttcccgt ttggcctgcc gattgatcag tgcgagtttc gccatctctg
     4081 tctcaccttg ttccgcatca gttgcggaag ggaaaccgga acgccgccag aagggccttg
     4141 gcctcttcgt cggacttcgc ggtggtcgtg atgctgatgt tcatgccccg gagcttgtcg
     4201 atcttgtcgt actcgatctc cggaaagatg atctgttcct tcaggccgat gttgtagttg
     4261 ccctggccgt cgaacgcgcg acccgacacg ccgcggaagt cgcgcacgcg cggaaaagcg
     4321 atcgtgacca gccgatcgag gaattcgtac atccgctggc cgcgcagcgt gaccatgcag
     4381 ccgatcgcca gcccctggcg aatcttgaag ttcgcaatcg ccttgcgcgc cttcgtcacc
     4441 accggcttct ggccggcgat cttggtcagg tcggccaccg acatgtcgat gttcttcttg
     4501 tcgttgaccg cttcgcccac gcccatgttc agcgtgacct tggtgatgcg cggtacctgc
     4561 atggtcgagg tgtagccgaa cttctggatc agctcgggaa cgatcttctc gcggtacagc
     4621 gtctgcaatc gagccatgtt cgcctcttac gccttggcgc cgaccacggt gccgccgccc
     4681 ttgaacacgc gcaccttctt gccgtcgacc accttgaatc cgacccgcgc gcccttcttg
     4741 gtcgccgggt tgaacagcat cacattcgac acgtcgatcg gcagcgtctt gtcgacgatg
     4801 ccgccggtca cgcccttcat cgggttcggg cggacatgct tcttgaccac gttgacgcct
     4861 tcgaccgtga cgtggcgctc gtcgatgcgc gccagcacga cgccgcgctt gcccttgtcc
     4921 ctgccggcga tgacgacgac ctcgtcgccc ttgcgaattc gatccattgc gtcgtctcct
     4981 acaggacttc cggcgccagc gacacgatct tcatgaagcg ctcggtgcgc agttcgcgcg
     5041 tcaccggccc aaagatgcgc gtgccgatcg gttccagctt tgggttcagc agcaccgccg
     5101 cgttagagtc gaacttgatc gacgagccat ccggccggcg caggccgtgc gcggtgcgca
     5161 cgacgaccgc gctgtaaacc tcgcccttct tcacgcggcc gcgcggcgcc gcgtccttga
     5221 ccgtcacctt gatgacgtcg ccgatgccgg cgtagcgccg tttgctgccg cccagtacct
     5281 tgatacacat caccgaacgc gcgcccgtat tgtccgcgac gtccagcatc gtctgcatct
     5341 gaatcatgat ttttcccaac ttttcccacc gcattgacgc ggcggtcagt cttggtcccg
     5401 tccgggagat gaatcagtcg gccttcggtc cgggacccgc tgccgcggag gacttccgac
     5461 ctgtctgact ccgaattcct cgcgcccaac ctaccggtgc gaagccaagc attatagctg
     5521 caattcccgc ctaaaccgtg gtcgccgcct ccacgacgcg ggtgaccatc cacgacttgg
     5581 tgcgcgagat cggccgggtc tcggcgattt caaccagatc accctcgttg acctggttcg
     5641 acgcgtcgtg cgcgtggaac ttggtcgaac gcaccacgta cttgccgtac atcggatgct
     5701 tgacgcgacg ctcgaccagc accgtcaccg tcctgtccat cttgttgctc accacgcgcc
     5761 cgaccagggt gcgcttcgtc ttggcttcgg tcgtcatttg gctgccgcct tctcggccat
     5821 cacggtgcgc acgcgcgcga tatcgcgctt gaccctacgc agctggctcg tgttctgcaa
     5881 ttgctgggtt gccttctgca tgcgcagcga gaaatgcgcg cgcagcagat cattcagttc
     5941 cttgctgagc gccgccgcgt ccttgccgcg aagttccttc gtttccatcg cttcttcctt
     6001 cgctactggc cgagctggcg gatcacgaac gtggtcttga tcggcagttt ggcggcggcc
     6061 agcgcgaacg cctcgcgcgc caccgattcg tccacgccgt ccatctcgta gatcaccttg
     6121 ccgggctgga tctgcgcgat ccagtactcg gggttgccct tgccgttgcc catgcggacc
     6181 tcggccggct tctgcgagat cggcttgtcc ggaaaaatgc ggatccacac gcggccgccg
     6241 cgcttgatgt ggcgcgagat cgcacgccgc gcggcctcga tctggcgcgc agtcagtcga
     6301 ccgcgttcgg tggccttcag gccgaattcg ccgaaggcga cgttcgcgcc gcgcgtcgcc
     6361 agcccgcggt tgcggccctt gtggtccttg cggtatttgc gtcgagcagg ttgcagcatc
     6421 gttattctcc ggacttgccg ccgccggtct tcgcgtcagc cgaggcagac ttggccgccg
     6481 ccttgcgcac gcgcttgacc gcgggcttgg gcgccgcttc ttccttggcc ggcgccggtg
     6541 tggcttctcc ttcgggcttg ccggcctcgg tccggcgcgc cggacgcttg ccggccggac
     6601 gacgcgcggc tgggcggcga tcctcgcgct ccggtgccgg ggtcggctcc gtcgccacgg
     6661 cgggcgcctc gttgcgaccc agcgtgtcgc ccttgtagac ccacactttc accccgatca
     6721 cgccgtaggt ggtcttcgct tccgagaagc cgt
//
LOCUS       DBNHBY010000061         1664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_611868, whole genome shotgun sequence.
ACCESSION   DBNHBY010000061 DBNHBY010000000
VERSION     DBNHBY010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_611868"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="AC16HB_01540"
     CDS             <1..1464
                     /locus_tag="AC16HB_01540"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR5605499.1"
                     /translation="PQLEVHVPPAAPAGAFELLARHRRAPVRTLPPQVEDIALLTHTS
                     GTTGLPKIMPFEHWRLTESARNRIELAAMEAADRSLSVIPFYSTVAIRRGLLPVLFSG
                     ASLVCPTKLDGASLVTLLGTLQPTQLLAPPVVQIAMLDEFERRTPRPRHALRFITSSF
                     AELTPAVRRRLEQAFGVPVIMSYGTAEAGSIAQSPFPPERTPDDAVGRAHFLKVAVTD
                     DAGRILGQDEVGEIVVRGPDVISGYENNEEANREAFRGGWFRTGDAGRIDRDGFVFLA
                     GRIKDIINRGGNKISPADVEAALQQHPQVAEAAAFAVPHPTLGEDVAAAVVLRDARLT
                     EFELRRFLGGRLAAVKLPSRILPLRELPRGTLGKVSRIELARIAQQAVTADFESPRNR
                     TEAEVACIFADVLELPRVSRSGNFFHLGGDSLRGVRVLASVESAFGIRPELDLLFDHP
                     AVADFAERIVAMAASEPSPAPPGPDGPTARQSTENTQ"
     gene            1461..>1664
                     /locus_tag="AC16HB_01545"
     CDS             1461..>1664
                     /locus_tag="AC16HB_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5605500.1"
                     /translation="MNKRRDTLLLLAGNALLRTPSLAFAQALPNRSVRIIVAYPPGGQ
                     ADSVARVTAQKLGELLDGSVVVEN"
BASE COUNT          255 a          573 c          569 g          267 t
ORIGIN      
        1 ccgcagctcg aggtgcacgt acctccggct gcacctgcgg gcgcgttcga actgctcgcg
       61 cgccatcgcc gggccccggt ccggacgctg ccgccgcagg tggaggacat cgcactgctc
      121 acccacacgt cgggaaccac cgggctgccc aagatcatgc cgttcgagca ctggcgtctg
      181 acggaatcgg cgcgcaaccg tatcgaactc gccgccatgg aggcggcgga tcggagtctg
      241 tccgtcattc ccttctattc gaccgtcgcg atccgccgcg ggctgctgcc ggtactgttc
      301 tccggcgcgt cgctggtgtg cccgaccaag ctcgatgggg cgtcgctggt gacgctgctg
      361 gggacgctgc agccgaccca gttgctcgcg ccgccggtgg tgcagatcgc gatgctggac
      421 gagttcgagc gccgcacgcc acggccgcgc catgcgctgc ggttcatcac ctcgagcttc
      481 gcggagctca cgccggctgt gcggcggcgg ctcgaacagg cattcggcgt tccggtcatc
      541 atgtcctatg gaacggcgga agccggcagc atcgcccagt ctccgtttcc tcccgaacgc
      601 acgccggacg acgcggtggg gcgcgcgcac tttctcaagg tggcggtcac cgacgatgcg
      661 ggtcgcatcc tcggacagga cgaggtcggc gaaatcgtgg tgcgcgggcc cgatgtgatc
      721 agcggctacg agaacaacga ggaggccaat cgcgaagcgt tccgcggcgg ctggttccgc
      781 accggcgatg cgggacgcat cgatcgcgac gggttcgtct ttctcgcggg ccgcatcaag
      841 gacatcatca accgcggcgg caacaagatc agccctgccg acgtcgaagc ggcgctgcag
      901 cagcatccgc aggtggcgga agcggcggcc tttgcggtgc cgcacccaac actgggagaa
      961 gacgtggccg ctgcagtcgt gctgcgcgat gcgcgcctga ccgaattcga gttgcggcgc
     1021 ttcctgggcg gtcggctggc cgccgtcaag ctgccctcgc gcatcctgcc gctgcgggaa
     1081 ttgccgcgcg ggacgctggg caaggtcagc cgcatcgagc tggcgcgcat cgcgcagcag
     1141 gcggtcaccg cggacttcga atcgcctcga aaccgtacag aggccgaggt cgcctgcatc
     1201 tttgcggacg tgctcgaact tccgagggtc agtcgatccg gcaacttctt ccacctcggc
     1261 ggcgactccc tgcgcggcgt gcgcgtattg gccagcgtgg agtcggcctt cggcatccgg
     1321 cccgaactgg atctgctgtt cgatcacccg gccgtggccg atttcgcgga aaggatcgtt
     1381 gcgatggccg cgagcgagcc gagtccggcg ccgcccggac cggacgggcc gacggctcgc
     1441 cagtcaacgg agaacaccca atgaacaagc ggcgcgacac gctgctgctg cttgcaggca
     1501 acgcgctact gcggacgccg agcctcgcct tcgcccaggc gctccccaac cggtcggtcc
     1561 gcatcatcgt tgcgtacccg ccgggcggac aggccgactc ggttgctcga gtcacagcgc
     1621 agaagctcgg cgagttgctc gacggttccg tcgtcgtcga gaac
//
LOCUS       DBNHBY010000062         3753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1492336, whole genome shotgun sequence.
ACCESSION   DBNHBY010000062 DBNHBY010000000
VERSION     DBNHBY010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1492336"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="AC16HB_01550"
     CDS             <1..587
                     /locus_tag="AC16HB_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5605501.1"
                     /translation="IIHALFFWVLASGLGAKIVETVMGPVETKIIEELPDEKDEPPPP
                     PPDIEQPPPYVPPPEVAVDIQIDTSNTTAISNATSERPVVQAPPPPPPAEKGPSVKTP
                     PSTQGRGARITQPEYPPASRRAGEAGTVQLRCFVKEDGRCSEVTIVKSSGFPKLDEAA
                     VKEVQRNWKFVPGKEDGKPVAMNHTFAVTFRLTD"
     gene            656..1378
                     /locus_tag="AC16HB_01555"
     CDS             656..1378
                     /locus_tag="AC16HB_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5605502.1"
                     /translation="MATETVSVENPYGIQALWAQGDFVAKGVLILLVLMSAYSWFIIF
                     TKWWEQRRLLSQAAEAEKQFWAADNIKAGLNKLTGKGNIFKAMAAEAIDAAEHHDSRL
                     TQQVPLHEWIDNVLARATARISSALSTQLPFLATTGSTAPFIGLFGTVWGIYHALVAI
                     GVAGQASIDKVAGPVGEALIMTAIGLAVAVPAVLGYNFLLGRNKQIVEKVKLFSNDLE
                     QVIVSGSRIEGAGARVIAGAKK"
     gene            1430..1867
                     /locus_tag="AC16HB_01560"
     CDS             1430..1867
                     /locus_tag="AC16HB_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5605503.1"
                     /translation="MNVTTGGGAPDDEKPMSEINTTPLVDVMLVLLIIFLITIPVITQ
                     SVRVELPKAANIPTQTKPENINIAVDKEGNVFWNTALVPTQDALLDRIKQVAVMDPQP
                     EIHVRGDRATAYENIGRVIVLCQRGGIQKVGFITEPDRGLARQ"
     gene            1891..2307
                     /locus_tag="AC16HB_01565"
     CDS             1891..2307
                     /locus_tag="AC16HB_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5605504.1"
                     /translation="MSMSIGGGGEDEPMMDINTTPLIDILLVLLIMFIITIPVMTHAV
                     KLDMPRANNAPSQTTPVVINLEIDFDGAVLWNGTPVTVDSLDTYFRREAAIDPQPELH
                     IRPNKRATYDVVARVLAAAQRNGLKKIGFVGNEQFM"
     gene            2438..3655
                     /locus_tag="AC16HB_01570"
     CDS             2438..3655
                     /locus_tag="AC16HB_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5605505.1"
                     /translation="MRKASFVLSTALAVAGWLGVAGTMLAVPATAYAQQKVSAKVGVP
                     LKAAQDAIQRKKWDAALAKIREADAIPGKTAYDQYKINEMLWYVYLQQGRNADAARVL
                     EQQLASGQTPAGERVQRTKTLAQLYARAGNYSKSASVAQQYLKSVPGDKDMQLLVANA
                     YYQQKNYKGAIAAAEQIMKGGGTPSQDLLQLVLRSNYELGDSAGTAKALEQLLKYYPS
                     KETWARVLDGYIKQTKHDDGLMSLYRLAEDVGTLNDARQYTDMTQALVVGGFGIEAER
                     IMQAGIAANLFAGEEMTRAQRTLEAAKRKAAAERAALPKAGTQLAAAKSGQEIARVGE
                     LYFSAGDYANAAATLQKALAKGGLEDTDRDQMLYGIALKRKGDKTGAMKAFDGIKDPK
                     FAEIARLWKTASR"
BASE COUNT          743 a         1249 c         1229 g          532 t
ORIGIN      
        1 tcattatcca cgcgctcttc ttctgggtcc tcgcctcggg cctgggcgcg aagatcgtcg
       61 agacggtgat gggaccggtc gagaccaaga tcatcgaaga gcttccggac gagaaggacg
      121 agccgccgcc gccgccgccg gacatcgaac agccaccgcc ttacgtcccg ccgccggaag
      181 tggccgtcga catccagatc gacacgtcca acaccacggc gatcagcaac gcaacatccg
      241 agcgtccggt cgtgcaggcg ccgcctccgc cgccgccggc ggagaagggt cccagcgtca
      301 agacgccgcc ttcgacgcag ggcaggggcg cgcgcatcac tcagcctgaa tatccacccg
      361 cgtcgcgccg tgccggcgag gcgggtacgg tgcagttgcg ctgcttcgtg aaggaagacg
      421 gccgctgcag cgaagtgacc atcgtcaaga gcagcggttt ccccaagctc gacgaagccg
      481 ccgtcaagga agtgcagcgc aactggaaat tcgtgcctgg caaggaagac ggcaagcccg
      541 tcgcgatgaa ccacaccttc gccgtcacgt tccgtctcac cgactgaccg ccttcgtatc
      601 catcttcatc cactgtccgc tacgccacta gaaacagaca caggaaccga tcgtcatggc
      661 caccgaaacc gtctccgttg aaaaccctta cggcattcag gccctctggg cccagggcga
      721 tttcgtcgcc aagggcgtgc tgatcctgct cgtcctgatg tcggcctact cctggttcat
      781 catcttcacc aagtggtggg agcagcgccg cctgctcagc caggcagcgg aggccgagaa
      841 gcagttctgg gccgccgaca acatcaaggc cggcctcaac aagctgaccg gcaagggcaa
      901 catcttcaag gccatggccg ccgaggcgat cgacgccgcc gagcaccatg actcgcgcct
      961 gacgcagcag gtcccgctgc acgagtggat cgacaacgtg ctggcccgcg ccactgcgcg
     1021 catcagcagc gcgctgtcga cgcagctgcc gttcctggcc accacgggtt caaccgcgcc
     1081 gttcatcggc ctgttcggca cggtgtgggg catctaccac gcgctggtcg ccatcggcgt
     1141 ggccggccag gcctccatcg acaaggtcgc cggaccggtc ggcgaagcgc tgatcatgac
     1201 cgccatcggc ctcgcggtgg ccgtgcccgc ggtgctcggc tacaacttcc tgctcggccg
     1261 gaacaagcag atcgtcgaga aggtcaaact gttctcgaac gacctcgaac aggtgatcgt
     1321 cagcggttcg cgcatcgaag gcgcgggcgc ccgcgtcatc gccggggcca agaagtaacc
     1381 gagccgggca gcccgcgtac agcggccgca agcaggtcca actatgtcta tgaacgtgac
     1441 tacgggcggt ggcgcgcccg acgacgagaa gccgatgtcg gagatcaaca ccacgcccct
     1501 cgtggacgtg atgctggtgc tcctcatcat cttcctgatc accatcccgg tgatcacgca
     1561 gtccgtgcgg gtggaactgc ccaaggccgc caacatcccg actcagacca agcccgagaa
     1621 catcaacatc gcggtggaca aggaaggcaa cgtcttctgg aacaccgcgc tcgtgccgac
     1681 acaggacgct ctgctcgacc gcatcaagca ggtcgccgtg atggatccgc agccggagat
     1741 ccacgtccgc ggtgaccgcg cgacggccta cgagaacatc ggccgcgtca tcgtgctgtg
     1801 ccagcgcggt ggcatccaga aggtcggctt catcacggag cccgaccgcg ggctcgcgcg
     1861 gcagtaggcc gggcaggaga ggatcgaacc atgtctatga gcattggcgg cggcggcgaa
     1921 gacgagccga tgatggacat caacaccacg ccgttgatcg acatcctgct ggtgttgctg
     1981 atcatgttca tcatcacgat cccggtgatg acgcacgcgg tgaagctcga catgccgcgt
     2041 gccaacaacg cgccgtcgca gaccaccccg gtggtcatca acctcgagat cgatttcgac
     2101 ggcgccgtgc tgtggaacgg cacgccggtg acagtcgact cgctcgacac ctatttccgg
     2161 cgcgaggcgg ccatcgatcc gcagcccgaa ctgcacatcc gcccgaacaa gcgggccacg
     2221 tacgacgtgg tcgcgcgcgt gctggcggcc gcgcagcgca acgggctgaa gaagatcggg
     2281 ttcgtcggca acgagcagtt catgtagcag acgacgggcg ttcgtccggc aggcggacaa
     2341 gccgccggcg gatcgaccct ggggagctgc cggtgcgaca agcaccggcg gcttttcgcg
     2401 tatcagaggg ccgcgcagcg gccgcagcga ggattcgatg aggaaggcga gtttcgttct
     2461 gagcaccgcg ctggcggtgg caggctggct gggcgtggcc ggcaccatgc tggccgtgcc
     2521 ggcgacggct tacgcacagc agaaggtgag cgcgaaggtc ggcgtgccgc tcaaggcggc
     2581 gcaggacgcg atccagcgca agaagtggga cgcggcgctc gccaagatcc gcgaagccga
     2641 cgccatcccc ggcaagacgg cgtacgacca gtacaagatc aacgagatgc tgtggtacgt
     2701 ctacctgcag caggggcgca acgcggatgc cgcgcgcgtc ctcgagcagc agctcgcgtc
     2761 gggccagacg ccggcgggcg agcgcgtgca gcgcaccaag acgctggcac agctttacgc
     2821 ccgcgccgga aactattcga agtcggcatc ggtcgcccag cagtacctga agtccgtccc
     2881 gggcgacaag gacatgcagc tgctggtcgc caatgcgtac taccagcaga agaactacaa
     2941 gggcgcgatc gccgcggctg agcagatcat gaagggcggc ggcacgccga gccaggacct
     3001 gctgcagctc gtgctgcgct ccaactacga gctcggcgat tccgcgggca cggcgaaggc
     3061 gctcgagcag ctgctcaagt actacccctc caaggaaacg tgggcccgcg tgctggacgg
     3121 ctacatcaag cagaccaagc acgacgacgg cctgatgtcg ctgtatcgcc tcgctgaaga
     3181 cgttggcacg ctgaacgacg cgcgtcagta caccgacatg acgcaggcgc tggtggtcgg
     3241 cggcttcggc atcgaggccg aacgcatcat gcaggcgggc atcgccgcga acctcttcgc
     3301 gggagaggaa atgacccgcg cccagcgcac gctggaggct gccaagcgca aggctgccgc
     3361 cgagcgcgcc gcgctgccga aggcgggaac gcaactggcg gccgccaaga gcggccagga
     3421 gatcgcgcgt gtcggcgagc tgtacttcag cgccggcgac tacgccaatg ccgcagccac
     3481 gctgcagaag gcgctcgcca agggcggcct cgaggacacg gaccgtgacc agatgctgta
     3541 cggcatcgcg ctgaagcgca aaggcgacaa gaccggcgcg atgaaggcgt tcgacggcat
     3601 caaggacccg aagttcgcgg agatcgccag gctctggaag acggcgtcgc gctgatcatc
     3661 cgtgacgagg ctgcgccgca cctgcgacgc agcacgcaat agaaaaggcc cgtggcgcaa
     3721 gccacgggcc ttttgcatgg gcgagggggt gcg
//
LOCUS       DBNHBY010000063         2519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1424091, whole genome shotgun sequence.
ACCESSION   DBNHBY010000063 DBNHBY010000000
VERSION     DBNHBY010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1424091"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16HB_01575"
     CDS             <1..233
                     /locus_tag="AC16HB_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5605506.1"
                     /translation="VFAVPDGTSPKIFARVQVDLLPTGEILAVKVLKPSGLPGYDAAV
                     ERAIRRTDPFPRKRDGTIDRTITIDFFPVDAQ"
     gene            287..1561
                     /gene="tolB"
                     /locus_tag="AC16HB_01580"
     CDS             287..1561
                     /gene="tolB"
                     /locus_tag="AC16HB_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR5605507.1"
                     /translation="MSKTLMRAAACAVLAWCSLAAAQLRVEITGVGSNQFPIAIANFQ
                     REGEVPQPVDEIVRADLQRSGLFRMVDTGGAALSETAAVNLNDWRGRGADALVVGSVF
                     RLADGRFDLRFRLYDTVKQAQLDGVSFVSTVAELRLNAHRIADRIYEKITGEKGVFAT
                     RIAYVVQLAKNSFELHVADADGANAQVALRSREPIMSPAWSADGSRLAYVSFESGKPV
                     VYTHTIASGERRAVANFRGSNSAPAWAPDGRSLAVVLTRDGISQVYSINADGSGLKRL
                     THSSAIDTEPVFSPEGKYIYFTSDRGGGPQIYRMAPDGSGVQRVTFSGDYNVSPRVSS
                     DGRLIAYIGRRGGRFQVYTLDLASNQEMALTDTAMDESPSFAPNGRMLLYATEVGGRG
                     ILATVSTDARVRTRLSGPSGDVREPTWGPFIK"
     gene            1587..2126
                     /gene="pal"
                     /locus_tag="AC16HB_01585"
     CDS             1587..2126
                     /gene="pal"
                     /locus_tag="AC16HB_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR5605508.1"
                     /translation="MLRFIGLAAAALLAAGCASTKLDEPAPVTDKSTAAKPAQPPGTG
                     TGADTRAVAPVDAQQRGDPLNDPNSPLAKRSVFFDFDSFVVKPEFQSLVEAHGKYLVA
                     NKSRRIVIEGNADERGSREYNLALGQKRADAVKSRLTLLGVSDGQVETVSFGEEKPRA
                     SGHDEASWAENRRADINYK"
     gene            2137..>2519
                     /locus_tag="AC16HB_01590"
     CDS             2137..>2519
                     /locus_tag="AC16HB_01590"
                     /inference="COORDINATES: protein motif:HMM:NF027655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HIR5605509.1"
                     /translation="MILDPRKLAIAVVAWGVFVAPAYALFGDDEARKAIIELRAKVAE
                     QEKQLQSKDAELAKRLDQLETANRAQLEFANTIESLRREIATLRGEVERLTREVATQQ
                     QRSRDLYGDIDARLKKLEPVAVAVDG"
BASE COUNT          430 a          854 c          858 g          377 t
ORIGIN      
        1 tcgtgttcgc cgttccggat ggcacttcac cgaagatatt cgcgcgggtt caggtcgacc
       61 tcctgccaac cggcgaaatc ctcgcggtta aagtgctgaa gccgagcggc ctgcccggct
      121 acgatgccgc ggtcgagcgc gcgatccgcc gcaccgaccc ctttccccgc aagcgcgacg
      181 gcacgatcga tcgcaccatc acgatcgatt tctttccggt tgacgcgcaa tgacgtgacg
      241 gccgttcgat cgtcccgaga gcgtggcttc ttataatttg acgcacatgt cgaagactct
      301 tatgcgcgcg gccgcctgtg ccgtgctcgc ctggtgctcg ctggctgccg cgcagctgcg
      361 ggtcgagatc accggcgtcg gctccaacca gttcccgatc gcgatcgcga atttccagcg
      421 cgaaggcgag gtgccgcagc cggtcgacga gatcgtgcgc gccgacctgc agcgctcggg
      481 gctgttccgc atggtcgaca cgggcggcgc ggcgctgtcc gagaccgcgg cggtgaatct
      541 caacgactgg cgcggccgcg gcgccgatgc gctggtggtg ggctcggtgt tccgcctcgc
      601 cgatggccgc ttcgacctgc gcttccgcct gtacgacacg gtcaagcagg cgcagctcga
      661 cggcgtgtcg ttcgtgtcga ccgtggccga gctgcggctg aatgcgcacc gcatcgccga
      721 tcgcatctac gagaagatca ccggcgaaaa gggtgtgttt gccacgcgca tcgcgtacgt
      781 ggtgcagctc gcgaagaaca gcttcgagct gcacgtggcc gacgccgacg gcgccaacgc
      841 gcaggtggcg ctgcgctcgc gcgagccgat catgtcgccc gcgtggtcgg cggacggatc
      901 gcggctcgcg tacgtttcct tcgaaagcgg caagccggtc gtctacacgc atacgatcgc
      961 cagcggcgag cggcgcgcgg tcgccaactt ccgcggcagc aacagtgcgc cggcgtgggc
     1021 gcccgacggc cggtcgctcg cagtcgtgct gacgcgcgac ggcatttcgc aggtctattc
     1081 gatcaacgcc gacggcagcg gactgaagcg gctcacgcat tccagtgcga tcgacaccga
     1141 acccgtcttc tcgcccgaag gcaaatacat ctacttcacg tcggaccgcg gcggcgggcc
     1201 gcagatctac cggatggcac ccgatggctc aggcgtgcag cgcgtgacct tcagcggcga
     1261 ctacaacgtc agcccgcgcg tgagcagcga cggccgcctg atcgcctata tcggccggcg
     1321 cggcggccgg ttccaggtct atacgctcga cctcgcatcc aatcaggaaa tggcgctgac
     1381 ggacaccgcg atggacgaat cgccgagctt cgcgcccaac gggcgcatgc tgttgtacgc
     1441 gaccgaggtc ggcggccgcg gcattctcgc caccgtctcc accgacgcgc gcgtgcggac
     1501 gcgcctgtcg ggcccgagcg gcgacgtgcg cgagccgacc tggggacctt tcataaagta
     1561 ggagaccact cgtgatgaca agaaccatgc ttcgcttcat cggcctcgct gccgccgcac
     1621 tgctcgccgc cggctgcgca tcgaccaagc tggacgagcc ggcgccggtc accgacaaga
     1681 gcaccgcggc caagccggcg cagccgcccg gcaccggcac cggcgccgac acgcgcgcgg
     1741 tcgcgccggt cgacgcacag cagcgcggcg atccgttgaa tgacccgaac agtccgctcg
     1801 ccaagcgcag cgtgttcttc gacttcgaca gctttgtcgt caagcccgag ttccagtcgc
     1861 tggtggaggc gcacgggaag tacctggttg ccaacaagag ccgccggatc gtgatcgaag
     1921 gcaacgccga cgagcgcggc agccgcgaat acaacctcgc gctggggcag aagcgtgccg
     1981 atgcggtcaa gagccggctg acgttgcttg gcgtcagcga cggccaggtc gagaccgtga
     2041 gcttcggcga ggagaagccg cgcgcgagcg gccacgacga agcgtcgtgg gcggagaacc
     2101 ggcgcgccga catcaattac aagtgagcgg tcgaccgtga tccttgatcc gcggaaactc
     2161 gcgatcgccg tcgtggcgtg gggcgtgttc gtcgcgcccg cgtatgcgct gttcggcgac
     2221 gacgaggcgc gcaaggcgat catcgaactg cgcgccaagg tcgcggagca ggagaagcaa
     2281 ctgcagtcga aggacgcgga actggcgaag cggctcgacc agctcgagac cgccaaccgc
     2341 gcgcagctcg agttcgccaa cacgatcgag tcgctgcggc gcgagatcgc cacgctgcgc
     2401 ggcgaagtcg agcgcctgac gcgcgaggtc gcgacgcagc agcagcgcag ccgcgacctg
     2461 tacggcgaca tcgacgcgcg gctgaagaag ctcgagccgg tcgcggtggc ggtcgacgg
//
LOCUS       DBNHBY010000064         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1830554, whole genome shotgun sequence.
ACCESSION   DBNHBY010000064 DBNHBY010000000
VERSION     DBNHBY010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1830554"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC16HB_01595"
     CDS             <1..199
                     /locus_tag="AC16HB_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5605510.1"
                     /translation="CLLAHHGVIACEDSLAAALRLAIEVENLAQTYLYARSLGEPPVL
                     SEDEMARVAARFRTYGQPRDA"
     gene            467..>1519
                     /locus_tag="AC16HB_01600"
     CDS             467..>1519
                     /locus_tag="AC16HB_01600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5605511.1"
                     /translation="MKIDQASLLSRVDPTRVFASADVALQQTKRRRYVPVRAKFLLAT
                     LAASAWCALSAWLSVPWLSDLAGALGLVIAVFLVGGIALLPGFMNAFLATSLALDRRP
                     LRRIPARYPALTILIAAYNEEESIYDTLASIEKQSYPAPLQVLVLDDGSQDRTVERVD
                     AFRAEHDAGWVELVPMPVNGGKARALNAGLMRAEHALVVTLDADSYLYRDALQHLVER
                     YLSDPIGTRAVAGTMLVRNSRQNWVTKAQEWDYFHGIAAIKRVQSLYQGTLVAQGAFS
                     LYERKALLEVGGWSDRVGEDIVLTWAMLARGWRVGHAEDACCFTRVPTTLPQFIRQRR
                     RWARGVFEALRQHPEIL"
BASE COUNT          242 a          516 c          496 g          265 t
ORIGIN      
        1 ctgcctgctt gcgcaccacg gtgtgatcgc ctgcgaggat tcattggccg ccgcgctgcg
       61 gctcgcgatc gaggtcgaga acctggcgca gacctatctg tacgcacgtt cgctcgggga
      121 gccgcccgtc ctttccgagg acgagatggc gcgcgtcgcc gcccgcttcc gcacgtacgg
      181 ccagccgcgc gacgcctgaa ccggctgacc ggccggcccg gaatgagccg gcgaggccgt
      241 tgttgcggcg cgacatccga gccacttcct tcggtcagac ctaggccacc gggcagaccg
      301 ccgctcgctt taataccaaa tatgcggtac tagatgcggg acaatcatcc cattgatgcg
      361 ggatgagacc cggtggctcg tctggccgtc cacctcgacc gcagtctcgt gagcgtgaac
      421 tcactctcac agcagcatcg agagccgata gaggcagaga gattccatga agatcgacca
      481 agcaagcctg ctcagccggg tcgatccgac gcgcgttttt gcctcggccg atgtcgcatt
      541 gcagcaaacg aagcgccgcc gctacgtgcc ggtgcgcgcg aagttcctcc tcgccacgct
      601 cgccgccagc gcctggtgcg cgttgtcggc atggctttcg gtaccgtggc tctccgatct
      661 ggccggtgcg ctcggactcg tgatcgcggt tttcctggtc ggcggcatcg cactactgcc
      721 cggcttcatg aatgcgtttc tcgccacgag tctcgccctg gaccgccggc ccctgcgccg
      781 aattccggct cgctatccgg cgctgacaat cctgatcgcc gcgtacaacg aggaggagtc
      841 gatctacgac acactggcgt cgatcgagaa gcagagctac cccgcaccgt tgcaggtact
      901 cgtgctcgac gacggctcac aggaccgcac ggtcgagcgt gtggacgcgt ttcgcgccga
      961 gcacgatgca gggtgggtgg aactagtgcc gatgccggtc aacggcggca aggcacgcgc
     1021 gctcaatgcc ggcctgatgc gcgcggagca cgcgctggtg gtgacgctcg acgccgactc
     1081 gtacctgtat cgcgacgccc tgcagcacct ggtcgagcgc tacttgtccg atccgatcgg
     1141 cacgcgcgcc gtggctggca cgatgctggt gcgcaactcg cgccagaact gggtcaccaa
     1201 ggcgcaggaa tgggactact tccacggcat cgcggcgatc aagcgcgtgc agtcgctgta
     1261 ccagggcacg ctcgttgcac aaggtgcgtt ctcgctgtac gagcgcaagg cgctgctcga
     1321 agtcggcggc tggtcggatc gcgtcggcga ggacatcgtg ctgacctggg cgatgctggc
     1381 gcgcggctgg cgcgttggtc atgccgagga cgcctgctgc ttcacgcgcg tgccgaccac
     1441 gctgccccag ttcatccgcc agcgccgccg ctgggcgcgc ggcgtcttcg aagcgctgcg
     1501 ccagcacccg gaaatcctg
//
LOCUS       DBNHBY010000065         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_544158, whole genome shotgun sequence.
ACCESSION   DBNHBY010000065 DBNHBY010000000
VERSION     DBNHBY010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_544158"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>2125
                     /gene="gyrB"
                     /locus_tag="AC16HB_01605"
     CDS             60..>2125
                     /gene="gyrB"
                     /locus_tag="AC16HB_01605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR5605512.1"
                     /translation="MSSADVYDSSKIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VLDNSIDEALAGYCTLITVTIHADQSVTVSDNGRGIPVDIHPEEGRSAAEVILTVLHA
                     GGKFDDSSYKVSGGLHGVGVSVVNALSETLYLAIHRDGKEYRQEYHLGDPVQPLAVVG
                     PSEQRGTTIRFKPSDEIFTNIEFEYEVLQKRLRELSFLNSGVRIELIDERDDRREGFH
                     HEGGIREFVRYLNRSKTSIHESIFWFKGERDGMGVEVALQWNDSYAETTYCFTNNIPQ
                     RDGGTHLAGFRGALTRTLNDYIEGELANKKDKVPTTGDDAREGLTAVLSVKLPDPKFS
                     SQTKDKLVSSEVKGVVESLVAEKLGEFLLENPGQAKAIVNKIIDAARAREAARKAREL
                     TRRKGALDIAGLPGKLADCQEKDPAKSELFIVEGDSAGGSAKQGRDRRTQAILPLKGK
                     ILNVERARFDKMLSSQEVGTLITALGCGIGKDELDLAKLRYHRILIMTDADVDGSHIR
                     TLLLTFFYRQIPELIERGHVYIAQPPLYKVKKGKTETYVKDDVELNQILLRSALDGAQ
                     LTVRAGAEPMPVEMLEALARRYLDFQNVIRRSPRRYDTHVLEQMMLTPEVALADREDS
                     DKLLAWGARLEQALNAVPRAGRTYTVTLQPGTPPHLVVTRIEHGIAVEKHLHREFFSS
                     SEYRRIT"
BASE COUNT          435 a          704 c          658 g          328 t
ORIGIN      
        1 ggaacacggc aaaatcgccc gtctgatata atcccgcgtc ccctcgaacc gggtatccca
       61 tgagctctgc ggacgtctac gactccagca aaatcaaagt cttaaaaggc cttgacgcgg
      121 tccgcaaacg ccccggcatg tacatcggcg acaccgatga cggcaccggc ctgcaccaca
      181 tggttttcga ggtactggac aactcgatcg acgaggcgct ggccggctac tgcaccctga
      241 tcaccgtcac catccacgcc gaccagtccg tcaccgtctc ggacaacggc cgtggaattc
      301 ctgtggatat ccatccggag gaggggcgtt cggcagcgga ggtgattctc accgtcctac
      361 acgccggcgg aaagttcgac gacagttcgt acaaggtctc aggcggcctg cacggcgtcg
      421 gcgtctcggt ggtcaatgcc ctgtccgaga ccctctacct ggcgatccat cgcgacggca
      481 aggagtaccg gcaggaatac cacctgggcg acccggtcca gccgctcgcc gtcgtgggcc
      541 ccagcgagca gcgtggcacg acgatccgct tcaagcccag cgacgagatc ttcaccaaca
      601 tcgagttcga gtacgaggtg ctgcagaagc gcctgcgcga actgtcgttc ctgaactcgg
      661 gcgtacgaat cgaactgata gacgagcgag acgatcgccg cgaagggttc caccacgaag
      721 gcggcatccg cgagttcgtg cgttacctga accgaagcaa aacttcgatt cacgaatcaa
      781 ttttctggtt caagggcgaa cgcgacggca tgggcgtcga ggtggcgctg cagtggaacg
      841 actcgtacgc cgagacgacc tactgcttca ccaacaacat tccgcagcgc gacggcggca
      901 cgcacctcgc cggtttccgc ggcgcactga cgcgcacgct gaacgactac atcgaaggcg
      961 aactcgccaa caagaaggac aaggtgccga cgacgggcga cgacgcgcgc gaaggcctca
     1021 cggccgtgct gtcggtcaag ctgcccgacc ccaagttctc gtcgcagacc aaggacaagc
     1081 tggtctcgtc ggaagtgaag ggcgtggtcg aatcactggt cgccgaaaag ctgggcgagt
     1141 tcctgctcga gaaccccggc caggccaagg cgatcgtcaa caagatcatc gacgcggccc
     1201 gcgcgcgtga agccgcgcgc aaggcgcgcg aactcacgcg ccgcaagggt gcgctcgaca
     1261 tcgccgggtt gcccggcaag ctcgccgact gccaggagaa agaccctgcg aagtccgagc
     1321 tgttcatcgt cgagggcgac tccgcaggcg gctcggccaa gcagggccgc gaccgtcgca
     1381 cgcaggccat cctgccgctg aagggcaaga tcctgaacgt cgagcgcgcg cgcttcgaca
     1441 agatgctctc gtcgcaggaa gtgggcacgc tgattacggc gctcggctgc ggcatcggca
     1501 aggacgagct cgacctcgcc aagctccgtt atcaccgcat cttgataatg accgacgcag
     1561 acgtggacgg aagccacatc cgtacacttt tgttgacttt cttttacagg cagatccccg
     1621 agctcatcga gcgtgggcac gtctacatcg cccagccgcc gctctacaag gtcaagaagg
     1681 gcaagaccga gacctacgtg aaggacgacg tcgagctcaa ccagattctg ctgcgctcgg
     1741 cgctcgacgg cgctcagctg acggtgcgtg ccggcgccga gccgatgcct gtcgagatgc
     1801 tcgaggcgct ggcgcgccgg tacctcgatt tccagaacgt catccgccgt tcgccgcgcc
     1861 gctacgacac gcacgtgctc gagcagatga tgctgacgcc ggaggtcgcg ctggccgacc
     1921 gcgaggattc ggacaagctg ctcgcctggg gcgcgcggct cgagcaggcg ctcaatgccg
     1981 tgccgagggc ggggcgcacg tacaccgtga cgctgcagcc cggcacgccg ccgcacctgg
     2041 tggtgacgcg catcgaacac ggcatcgccg tcgagaagca cctgcaccgc gagttcttca
     2101 gctcgagcga gtaccgccgc atcac
//
LOCUS       DBNHBY010000066         1848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_70917, whole genome shotgun sequence.
ACCESSION   DBNHBY010000066 DBNHBY010000000
VERSION     DBNHBY010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_70917"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..>1848)
                     /locus_tag="AC16HB_01610"
     CDS             complement(247..>1848)
                     /locus_tag="AC16HB_01610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR5605513.1"
                     /translation="NGYLPALRHANASVYLVQPIVRNRRVWGLIVLGHSESVALSPRR
                     RALIRDVADRVAVALATVERDRRLHVMAHLDGLTGLPNRQHLQTILEQELAQAKAAGT
                     RAAVLFIDLDRFKQTNDTLGHSAGDTLLKRASERIRASVRESDQVGRHGGDEFTVILN
                     GIDAANDAAHVARKLVKAIDKPFEVEGHSVYVGASVGIAIYPDDGESGAELVKKADTA
                     MYRAKETGRGRFAFFEKTMNVEMNRRVTLDRELRQAIENNQFELHYQPQIELASGRIC
                     AAEALLRWRHPKRGLLSPIAFIELAEETGLIEQLGSWVLREACVQHGRWIAEGVRIPR
                     VAVNVSSRQLRQADFVPSVYYTMAATHMAPDSLEIEVTESLILDGGEAAIAALRALEN
                     AAARVAIDDFGTGYSSFTYLKTLPASILKLDKSFMLNVTTDKDAATIAAAIINMAHTL
                     RKEVVAEGVETAEQLEFLRRAGCEKVQGHHLSPPLPANKLAELARLSEREGLDAVLEI
                     TRQRMDRIAARAAQKKEGTRAAETA"
BASE COUNT          275 a          638 c          606 g          329 t
ORIGIN      
        1 gtgcccctgc agctgctcgc gtaccacacg gcggtcgcgc gtgggacgga tgtcgacaaa
       61 ccccgaaatc tggcgaaatc cgtgacggtc gagtaagcga ctcgaagcgg gtgccgagcc
      121 cggcacccgg tttcggggtt tgcggagcag gctgacttgc cgcaggcaag tcagggcggt
      181 gcagtcgccg gccggagaaa caacccgcga aactcgccgc aatccaccac ggtccagcga
      241 ttgtcctcag gcggtctctg ccgcccgtgt gccttccttc ttctgcgcgg cgcgcgcggc
      301 gatccggtcc atccgctgcc gggttatctc gagcacggca tccagaccct cgcgctccga
      361 cagtcgcgca agttcggcca gcttgttggc cggcaatggt gggctcaggt ggtggccctg
      421 caccttttcg cagccggcgc ggcgcaggaa ctccaactgc tcggcggtct cgacgccttc
      481 ggccacgact tccttgcgca gcgtgtgcgc catgttgatg atcgcggcgg cgatcgtggc
      541 ggcatccttg tcggtggtca cgttgagcat gaaggacttg tccagcttca ggatcgatgc
      601 cggcagcgtc ttcaggtagg tgaacgacga atagccggtg ccgaaatcgt cgatcgccac
      661 gcgcgccgcc gcgttctcca gcgcgcgcaa tgccgcgatc gccgcttcgc cgccgtcgag
      721 gatcagcgac tcggtgacct cgatctccag gctgtccggc gccatgtgcg tcgccgccat
      781 cgtgtagtac accgatggca cgaagtccgc ctgccgcagt tgccggctcg acacgttcac
      841 cgccacgcgc gggatgcgca cgccctcggc gatccagcgc ccgtgctgca cgcaggcctc
      901 gcgcagcacc caggagccga gctgctcgat caggccggtc tcctcagcca gttcgatgaa
      961 cgcgatcggc gacagcagcc cgcgcttcgg gtggcgccag cgcagcagcg cctctgcagc
     1021 gcagatgcgg ccggaggcga gctcgatctg cggctggtag tgcagctcga actggttgtt
     1081 ctcgatcgcc tgccgcagtt cgcggtcgag cgtcacgcgc cgattcatct ccacgttcat
     1141 cgtcttttcg aagaacgcga agcgtccgcg cccggtttcc ttggcgcgat acatcgcggt
     1201 atcggccttc ttgaccagct cggcaccgga ctcgccgtcg tctggataga tcgcgatgcc
     1261 cacgctggcg cccacgtaga cgctgtggcc ttcgacctcg aacggcttgt cgattgcctt
     1321 gaccagtttg cgcgcgacgt gggcagcgtc gttggccgcg tcgatgccgt tcaggatcac
     1381 cgtgaattcg tcgccaccgt ggcgcccgac ctgatcgctc tcgcgcacgc tggcgcggat
     1441 gcgctctgac gcgcgcttga gcagcgtgtc gccggccgag tggccaagcg tgtcgttggt
     1501 ctgcttgaag cgatccaggt cgatgaacag caccgccgcg cgggtaccgg cggccttggc
     1561 ctgcgccagc tcctgctcga ggattgtctg caggtgctgc cggttcggca atccggtcag
     1621 accgtccaga tgcgccatca cgtgcaggcg ccggtcgcgt tcgacggtgg ccagcgcgac
     1681 ggctacgcga tcggcgacgt cccggatcag cgcgcgtcgg cgcggcgaca gggccaccga
     1741 ctcgctgtgg ccgagcacga tcaaccccca cacgcgccga ttgcgcacga tcggctgcac
     1801 cagatacacc gacgcgttcg cgtggcgcag cgccggcagg tatccatt
//
LOCUS       DBNHBY010000067         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1153787, whole genome shotgun sequence.
ACCESSION   DBNHBY010000067 DBNHBY010000000
VERSION     DBNHBY010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1153787"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="AC16HB_01615"
     CDS             complement(<1..540)
                     /locus_tag="AC16HB_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5605514.1"
                     /translation="MTRVAIVGAGAIARGMAALLARGGHEVGVWSPSGASTAAWLGSE
                     VGFAWPGASVRPAALNYAGVASGSAHRAVLPDAAAIAGADVVAIALPANGYASVLPPV
                     AAALRDEHLVLFSGALSLAPLWLAELAARRGARPTIASFGTTIVTARSEPAGVRIMTL
                     RTRLDVAAIPARAGKRARAI"
     gene            complement(537..1511)
                     /locus_tag="AC16HB_01620"
     CDS             complement(537..1511)
                     /locus_tag="AC16HB_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5605515.1"
                     /translation="MLNHRRLALAILACAALAPALAFAQAYPSKPIKLVVPFTPGGSQ
                     DVIGRLLAQRVGESLGQQIVIENQAGAGGQIATQSVARAAPDGYTLLLSTGAQMAIEP
                     NLRPNIGYDPLRDFAHVVHVGDTPLVLLAIPSFAPQTVKDLIDYSKANKGKVNTASTG
                     NGTYTHLTLELFKAATGADLTHIPYKGAAPAFSDLLGGAVQTMFTTIASAQPHLVSGK
                     LRALAVTANKRSALLPGVPTFAEAGVPNLEVSVWIGISAPAGTAPAIVERLGKEFAAA
                     LARPDVRERLAALGVEPAGAQSAAFTQMVRADIERWARVIKSANIKVE"
     gene            1571..2365
                     /locus_tag="AC16HB_01625"
     CDS             1571..2365
                     /locus_tag="AC16HB_01625"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR5605516.1"
                     /translation="MHQYEDFMRHVFEHGADKRDRTGTGTRSVFGYQMRFPLREGFPL
                     VTTKKLHMRSIIHELLWFLRGDSNVRYLHENNVTIWDEWARPDGELGPVYGVQWRSWP
                     TPHGGHIDQMAQVLEQIRKNPDSRRLIVSAWNVGDIPKMALPPCHLLFQFYVANGALS
                     CQLYQRSCDIFLGVPFNIASYALLTHMVAQQCDLEVGDFVWVGGDCHIYGNHFEQVRE
                     QLAREPKPYPRLQIKRRPPTLFGYRYEDFEFVGYDPHPHIKAPVAV"
     gene            2365..2898
                     /locus_tag="AC16HB_01630"
     CDS             2365..2898
                     /locus_tag="AC16HB_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5605517.1"
                     /translation="MPRIRYRDADDPAIAELVAAIRARRGGKLLNLDRMLLVSPPYAR
                     GWNALLGAVRRELELSLRIRELAICAVAKLNRAAYEWQQHAPEFRAAGGRPDQLDALE
                     DVEAASTDARFDDAERAALALTLDMTRAVAVRPDTFARAQRFFTERQLVELVGTIATY
                     NMVSRFLVALDVTQDGE"
BASE COUNT          441 a         1022 c         1022 g          427 t
ORIGIN      
        1 gatggcccgc gcgcgtttcc cggcgcgcgc cggaatcgcc gctacgtcga gccgcgtgcg
       61 cagggtcatg atgcgcacgc cggcgggctc gctccgcgcg gtgacgatcg tggtgccgaa
      121 cgaagcgatc gtcggccgcg cgccgcgccg cgccgcgagc tccgccagcc acagcggcgc
      181 cagcgacagc gccccggaaa acagcacgag gtgttcgtcg cgcagcgccg cggcgaccgg
      241 gggcagcacc gatgcgtagc cgttggcggg cagcgcaatc gcgaccacgt cggcgccggc
      301 gatcgcggcg gcgtccggca gcacggcacg atgcgcgctg ccgctcgcga cgccggcata
      361 gttcagcgcc gcagggcgca cggacgcgcc cggccatgcg aacccgacct ccgacccgag
      421 ccacgccgcc gtgctcgcac ccgacggcga ccacacgccg acctcgtggc caccgcgcgc
      481 cagcagcgcg gccatgcccc gcgcgatcgc gcccgcgccg acgatcgcga cgcgcgtcat
      541 tcgaccttga tgtttgcgct cttgatgacg cgtgcccagc gctcgatgtc ggcgcggacc
      601 atctgcgtga aggcggcgct ctgcgcgccc gccggctcga cgccgagcgc agcgagccgc
      661 tcgcgcacgt cgggcctggc cagtgcggcg gcgaactcct tgccgagccg ctcgacgatc
      721 gcgggggcag tgcccgccgg cgcggagatg ccgatccaca ccgacacctc gaggttcggc
      781 acgccggcct cggcaaacgt cggcacgccg ggcagcagcg cgctgcgctt gttcgcggtc
      841 accgcgagcg cgcgcagctt gccggacacg aggtgcggct gcgccgaggc gatcgtcgtg
      901 aacatcgtct gcaccgcgcc gccgagcagg tcgctgaacg ccggcgccgc gcccttgtac
      961 ggaatgtgcg tgaggtcggc gccggtggcg gccttgaaca gctccagcgt caggtgcgtg
     1021 taggtgccgt tgccggtcga cgccgtgttg accttgccct tgtttgcctt cgagtaatcg
     1081 atcaaatcct tgaccgtctg cggcgcaaac gacgggatcg ccagcagcac cagcggcgtg
     1141 tcgccgacgt gcaccacgtg cgcgaagtcg cgcagcgggt cgtagccgat gttggggcgc
     1201 aggttcggct cgatcgccat ctgcgcgccg gtcgacagca gcagcgtgta gccgtcgggc
     1261 gccgcgcgcg cgaccgactg ggtcgcgatc tggccgccgg cgccggcctg gttctcgatc
     1321 acgatctgct ggccgagcga ctcgcccacg cgctgcgcga gcaggcgccc gatcacgtcc
     1381 tgcgagccgc cgggggtgaa ggggacgacg agcttgatgg gcttggaggg atatgcctgc
     1441 gcgaaggcaa gcgccggggc gagcgctgcg caggcgagga tggcgagcgc cagacgtcga
     1501 tggttcagca tggtcttccg tcgttcgatg tggtggccga tagaatgcag acgattatct
     1561 cccgaccgcc atgcaccagt acgaagactt catgcgccat gtgttcgagc atggcgccga
     1621 caagcgcgac cgcaccggca ccggcacacg ctcggtgttc ggctaccaga tgcgcttccc
     1681 gctgcgcgag ggcttcccgc tcgtcaccac caagaagctg cacatgcgct cgatcatcca
     1741 cgagctgctg tggttcctgc gcggcgattc gaacgtccgc tacctgcacg agaacaacgt
     1801 gacgatctgg gacgagtggg cgcgccccga cggcgaactc ggcccggtgt acggcgtgca
     1861 atggcgctcg tggccgacgc cgcacggcgg ccacatcgac cagatggcgc aggtgctgga
     1921 acagatcagg aagaaccccg attcgcgccg gctgatcgtg tcggcgtgga acgtcggcga
     1981 cattccgaag atggcgctgc cgccgtgcca tctgctgttc cagttctacg tggcgaacgg
     2041 tgcgctgtcg tgccagctgt atcagcgcag ctgcgacatc ttcctcggcg tgcccttcaa
     2101 catcgcgtcc tatgcgctgc tcacgcacat ggtcgcgcag cagtgcgatc tggaagtcgg
     2161 cgacttcgtg tgggtcggtg gcgactgcca catctacggc aaccacttcg agcaggtgcg
     2221 cgagcagctg gcgcgcgagc cgaagcctta tccgaggctc cagatcaagc gccggccgcc
     2281 gacgctgttc ggctaccgct acgaggactt cgagttcgtc ggctacgacc cgcacccgca
     2341 catcaaggcg ccggtggcgg tctgatgccg aggatccggt accgcgatgc ggacgatccc
     2401 gcgatcgccg aactggtcgc cgcgatccgc gcgcgccgcg gcggcaagct gctgaacctc
     2461 gaccgcatgc tattggtgag cccgccgtat gcgcgcggct ggaacgcgct gctcggcgcg
     2521 gtgcggcgcg agctggaact gtcgctgcgc atccgggaac tggcgatctg cgcggtggcc
     2581 aagctcaatc gcgccgcgta cgaatggcag cagcacgcac ccgagttccg cgccgcgggc
     2641 ggacgccccg accaactcga cgcgctcgaa gacgtcgagg ccgcgagcac cgatgcgcgc
     2701 ttcgacgatg cggagcgcgc ggcgctcgcg ctgacgctgg acatgacgcg cgcggtcgcg
     2761 gtgcggccgg acacgtttgc ccgtgcgcag cggttcttca ccgaacgcca gctcgtcgag
     2821 ctggtcggca cgatcgcgac ctacaacatg gtgtcgcgct tcctggtcgc gctcgacgtg
     2881 acgcaggatg gcgagtagcg atcagccgcg cg
//
LOCUS       DBNHBY010000068         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_409386, whole genome shotgun sequence.
ACCESSION   DBNHBY010000068 DBNHBY010000000
VERSION     DBNHBY010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_409386"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="AC16HB_01635"
     CDS             complement(<1..622)
                     /locus_tag="AC16HB_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605518.1"
                     /translation="MNARDELAALERAAAPGRPVLLTGNHAFAWAARLARPKVVPVYP
                     ITPQTPVLELITEFQAEGKFDAEIITPESEHSVMAACIPASLAGVRVFTATASQGLLL
                     MHELLHYASGARAPIVMANINRTVASPWAFWPDQTDSLAQRDTGWIQFYSESAQESLD
                     TTLHAFRIAERVLLPAMINHDAFYISHALEPVAIPAQELVDRFLPPY"
     gene            complement(693..2339)
                     /locus_tag="AC16HB_01640"
     CDS             complement(693..2339)
                     /locus_tag="AC16HB_01640"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR5605519.1"
                     /translation="MNAPIPPIWTTGSTEVFKTGTWRAALPQHIRAPSPCHAACPVNG
                     DIAEWIGRARNRDYRGAWEVLVRNNPFPAIAGRICHHPCESACNRTGFDEPLAICKLE
                     RFVGDLALAEGWTHDKPAAHHSERIAVVGGGPSGLSAAFQLARRGYRVTLFESQPELG
                     GLMRYGIPSYRLARSVLDGEIARIVALGVEVRCGESLDAPADLERLRSEFDAVYVAIG
                     ARRQKRLAQLDYTQSWVMDGADYLARSARGEKPALGKRVVVIGGGSAALDAARSARRA
                     GHDVTLLALEKAAQMPAQREEIQEALEEGILLVAGAMLTEVVPRLDDGVRLNCVRVRF
                     EPGLQRGQFTVTPIADSEFVLEADAIVPSIGQDPDLTALQALDRAGALLNVDARQATS
                     AERVYAGGDVSSMARFVTEAIGMGKRAALEIDRVLRAGDAAGNASTESIVPLGAIATA
                     YYAKQARPAERHLDPAQRLKSELEVQLGFDVDTALAETERCFSCGTCILCDNCVNVCP
                     DLAVKRSGAGYLVLADYCKGCGLCVKECPTGSMKMVEEVR"
     gene            complement(2336..2893)
                     /locus_tag="AC16HB_01645"
     CDS             complement(2336..2893)
                     /locus_tag="AC16HB_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5605520.1"
                     /translation="MLQIMIQGRGGQGAQTAGNLLAMAFFAEGRQVQAFSSYGGARRG
                     TPVNSYIRVDDRPVRLRCDIENADAILCFDASLLQGPLLAVARPDTLIVVNSAKTPAE
                     YARTLPGYRVIPVDGLGISRRNGLGRIVNSALLGGFARAIEAPSLDALTRTLTEEAPK
                     LHDENIAACEEGWRWVDAQMQRAAA"
BASE COUNT          444 a         1036 c         1027 g          478 t
ORIGIN      
        1 catacggcgg caggaagcgg tcgaccagtt cctgcgcggg aatcgcgact ggctccagcg
       61 cgtgcgagat gtagaacgca tcgtggttga tcatcgcggg aagcaggacg cgctcggcga
      121 tccggaatgc gtgcaatgtc gtgtcgagcg actcctgtgc gctctcggaa tagaactgga
      181 tccagccggt gtcacgctgc gccaggctgt cggtctggtc gggccagaac gcccacggcg
      241 aggccaccgt gcggttgatg ttcgccatca cgatcggcgc gcgcgcgccg ctggcgtaat
      301 gcagcagctc gtgcatcagc agcagcccct gcgacgcggt cgccgtgaac acgcgcacgc
      361 cggcgagcga cgccgggatg cacgcggcca tcaccgagtg ctcggactcg ggcgtgatga
      421 tctcggcatc gaacttgccc tcggcctgga actcggtgat cagctccagc accggcgtct
      481 gcggcgtgat cggatagacc ggcacgacct tcggccgcgc aagcctggcc gcccatgcaa
      541 acgcgtggtt gccggtcagc agcacgggcc ggccgggcgc tgcggcgcgt tcgagcgcgg
      601 ccagttcgtc tcgcgcgttc atgttgcccc cttcgccctg cgggctgtcc ccccgagggg
      661 gagcgagcgc cttcggagcg gccgtgcggc actcatctca cctcctcgac catcttcatc
      721 gagccggtcg ggcattcctt cacgcacagg ccgcagccct tgcagtagtc ggcgagcacg
      781 aggtagccgg caccactgcg cttcaccgcg agatcggggc agacgttcac gcagttgtcg
      841 cacaggatgc aggtgccgca ggagaagcag cgctcggtct cggcgagcgc ggtgtcgaca
      901 tcgaagccga gctgcacttc gagttcgctc ttcagcctct gcgcggggtc gagatggcgt
      961 tcggccggcc gcgcctgctt tgcgtaatac gcggtggcga tcgcgccgag cgggacgatc
     1021 gactctgtgc tcgcattccc ggcggcatcg cccgcgcgca ggacgcggtc gatctccagc
     1081 gccgcgcgct tacccatgcc gatcgcctcg gtcacgaagc gcgccatgct cgatacgtcg
     1141 ccacccgcgt atacgcgctc ggcgctggtg gcctggcgcg cgtcgacgtt cagcagcgcg
     1201 cccgcgcgat ccagcgcctg caacgcggtg aggtccggat cctggccgat cgacggaacg
     1261 atcgcgtcgg cctcgagcac gaattcgctg tcggcgatcg gcgtcaccgt gaattgaccg
     1321 cgctgcagtc cgggctcgaa gcgcacccgc acgcagttca gtcgaacgcc gtcgtccagg
     1381 cgcggcacga cctcggtcag catcgccccg gcaaccagca agatgccctc ctccagcgct
     1441 tcctggattt cctcgcgctg cgcgggcatc tgcgcggcct tctccagcgc gaggagcgtg
     1501 acgtcgtggc cggcgcggcg agcgctgcgc gcggcgtcga gcgccgcgct gccgccgccg
     1561 atcacgacca cgcgcttgcc gagcgcgggc ttctcgccgc gggccgaacg cgccaggtag
     1621 tcggcgccgt ccatcaccca cgactgcgtg tagtcgagct gcgccagtcg tttctgtctg
     1681 cgcgcgccga tcgcgacgta aaccgcgtcg aattcgctgc gaagccgctc gaggtcggcc
     1741 ggcgcgtcga gcgattcgcc acagcgcact tcgacgccga gcgcgacgat gcgggcgatc
     1801 tcgccgtcga gcacgctgcg cgcgagccga tacgacggaa tgccgtagcg catcagcccg
     1861 ccgagttccg gctgcgattc gaacagcgtc acccggtagc cgcgccgcgc gagctggaag
     1921 gccgcggaca ggcccgacgg cccgccgccg acgacagcga tgcgctcgct gtggtgcgcg
     1981 gcaggcttgt catgggtcca cccttccgcg agcgccaggt cgccgacgaa gcgttccagc
     2041 ttgcagatcg ccagcggttc gtcgaatccg gtgcggttgc acgcggactc gcacgggtgg
     2101 tggcagatgc ggccggcgat cgccgggaac ggattgttgc ggacaagaac ttcccacgca
     2161 ccgcgatagt cgcgattgcg cgcgcggccg atccactcgg cgatgtcgcc gttcaccggg
     2221 caggcggcgt ggcacggcga cggggcgcgg atgtgctgcg gcagcgcggc gcgccacgtg
     2281 ccggtcttga agacttcggt cgagccggtg gtccagatcg gcgggatcgg tgcattcatg
     2341 ccgcggccct ctgcatctgc gcatcgaccc agcgccagcc ctcttcgcac gcggcgatgt
     2401 tctcgtcgtg cagcttgggg gcctcctccg tcagcgtgcg cgtcaacgcg tcgagcgacg
     2461 gtgcttcgat tgcgcgcgcg aaaccgccga gcagcgcgga gttgacgatc cgccccagcc
     2521 cgttgcggcg tgaaatgccg agcccgtcga ccgggatcac gcggtagccc ggcagcgtcc
     2581 gcgcatactc ggccggggtc ttcgcggagt tgacgacgat cagcgtgtcg ggccttgcga
     2641 cggcgagcag tggcccctgc aacaggctgg cgtcgaagca caggatcgcg tcggcattct
     2701 cgatgtcgca gcgcagacgg accggtctgt cgtcgacgcg gatgtatgaa ttgacgggcg
     2761 taccgcggcg cgcgccgccg tagctggaaa acgcctgtac ctgccggccc tcggcgaaga
     2821 acgccatcgc cagcagattg cccgcggtct gcgcgccttg gccgccgcgg ccctggatca
     2881 tgatctgcag cacgtttgtc tcctcgtgcg cgccaatcta ggccgccgct cgccattcgt
     2941 ccaattgatt gtcagaatgc ggcgcattca cgaatcagat aggag
//
LOCUS       DBNHBY010000069         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1559944, whole genome shotgun sequence.
ACCESSION   DBNHBY010000069 DBNHBY010000000
VERSION     DBNHBY010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1559944"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="AC16HB_01650"
     CDS             <1..755
                     /locus_tag="AC16HB_01650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5605521.1"
                     /translation="LPIFDVHMHYSHDAWDNVPPKVALDLLQQAGVKRALVSSSNDDG
                     QQRLRQQAPDVIVPSLRPYRTRGDVGRWARDDSVIPYLEERLARFRYVAMGEFHIYGA
                     DVDLPVPRRMIELARKHDLVLHSHSDADAIEREFAQWPGARILWAHAGFVPPAAVRAI
                     LARHRSLWCDLAFRTDHAPGGQLDPEWRALFMEFPDRFMVGTDTFTPERWPYVVEHAR
                     WSRQWLAQLPADVAERIAWKNAEALLLRETKP"
     gene            752..1150
                     /locus_tag="AC16HB_01655"
     CDS             752..1150
                     /locus_tag="AC16HB_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605522.1"
                     /translation="MRPFALAAGLIGFAASALACEPALRGAGVQTVEGQTYVVAWRAS
                     APIEVGDFFELEVAACSRRANALATLRVDAQMPEHRHGMNYKPAVSTRGDGRFVASGF
                     LLHMPGRWEFTFDLRGADGAETLRTSVNVP"
     gene            1147..2328
                     /locus_tag="AC16HB_01660"
     CDS             1147..2328
                     /locus_tag="AC16HB_01660"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR5605523.1"
                     /translation="MNARILALALLLAGVAIAQGPQPDFTPNERARILAHGPWPPAIT
                     RDPSNRMSGNPAAIDLGRRLFSDSALSRSGYIGCVTCHQPDRALTDLRARAHGMADVD
                     RNTPTLANLRLQRWYGWGGASDSLWMASIRPMLDARELDSDAAVIARRFGLNDDLSCR
                     YRKVFGRAPDHADDELLANVGKALAAFEETLVTGRTPFDDFRDALERNDQAAMRRYPA
                     AARRGLQLFVGRANCFVCHTGPNFSNGEFHDVGIPFFVAKGKVDPGRHEGIRIVQASR
                     FSLLGRFNDDAGGASATATRHVVLEHRHWGEFRTPSLRNVAVTPPYMHNGSLATLRDV
                     VLHYSELDGERLHADGEQILRKLNLTEEEIGDLVAFLDTLTDAKGERRLLPPIDPRAC
                     E"
     gene            2432..2884
                     /locus_tag="AC16HB_01665"
     CDS             2432..2884
                     /locus_tag="AC16HB_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605524.1"
                     /translation="MVRRLALALIPFAFAGCATVTGSPTQSISIHTVDASGKPVNGMR
                     CRIANGNADYYGNSPLLDMQVRRSGADLEIECRSGSRVASATAFARSLSLTGAVLLPG
                     GGAAALIDRLSGYAYAYPRSMQLRVGEHMIFDVSAPAREGDESADSLR"
BASE COUNT          431 a          997 c         1005 g          451 t
ORIGIN      
        1 agctgcctat attcgacgtc cacatgcact actcgcacga cgcctgggac aacgtgccgc
       61 cgaaggtcgc gctcgacctg ctgcagcagg cgggcgtgaa acgtgcgctg gtgtcgagct
      121 ccaacgatga cggccagcag cgtttgcgtc agcaggcgcc cgatgtgatc gtgccgtcgc
      181 tgcggccgta ccgcacccgc ggcgacgtcg gccgctgggc gcgcgacgac tcggtgattc
      241 cgtacctgga agagcggctc gccaggttcc gctacgtcgc gatgggcgag ttccacatct
      301 acggtgccga cgtcgatctg ccggtgccgc ggcggatgat cgagctggca cggaagcacg
      361 atctggtgct gcactcgcat tcggacgccg acgcgatcga gcgcgagttt gcgcaatggc
      421 cgggcgcgcg catcctgtgg gcgcatgccg gtttcgtgcc gccggccgcg gtgcgcgcga
      481 ttctggccag gcatcgcagc ttgtggtgcg acctcgcgtt ccgcaccgat cacgcaccgg
      541 gcggccagct cgatccggag tggcgcgcgc tcttcatgga gtttcccgac cgcttcatgg
      601 tcgggaccga tacctttacg cccgagcgct ggccgtacgt ggtcgagcat gcgcgctggt
      661 cgcgtcaatg gctcgcgcag ctgcctgccg acgtcgccga gcgcatcgcg tggaagaacg
      721 ccgaggcgtt gctgctgcgg gagacgaagc catgagaccg ttcgcgcttg cggcagggct
      781 gatcggcttt gcggcgtcgg cgctcgcgtg cgagcccgcg ctgcgcggcg ccggcgtgca
      841 gacggtcgaa gggcagacct acgtcgtcgc ctggcgcgcg agcgcgccga tcgaggtggg
      901 cgactttttc gagctcgagg tggcggcgtg ttcgcgccgc gccaacgcgc tggccacgtt
      961 gcgcgtcgac gcgcagatgc ccgagcatcg gcacggcatg aactacaagc cggcggtcag
     1021 cacgcgcggc gacggccgct tcgtggcgag cgggtttctg ctccacatgc cgggccgctg
     1081 ggagttcacg ttcgacctgc gcggcgccga cggtgccgag acgttgcgca cgagcgtgaa
     1141 cgtgccgtga acgcgcgcat ccttgcgctg gcgctgctgc tggccggtgt cgcgatcgcg
     1201 caggggccgc agcccgactt cacacccaac gagcgcgcgc gcatcctcgc gcacggcccg
     1261 tggccgcctg caatcacgcg cgacccgagc aatcgcatgt cgggcaatcc ggccgcgatc
     1321 gatctcggcc gccgcctgtt ctcggattcc gcgctgtcgc gcagcggcta tatcggctgc
     1381 gtgacgtgcc atcagcccga ccgcgcgctg acggatctgc gcgcccgcgc gcacggcatg
     1441 gccgatgtcg atcgcaacac gccgacgctc gccaacctgc ggctgcagcg ctggtacggc
     1501 tggggcgggg ccagcgacag cctgtggatg gccagcatcc ggccgatgct cgacgcgcgc
     1561 gaactcgact cggacgcggc cgtcatcgcg cgacgcttcg ggctcaacga tgatctttcc
     1621 tgccgatacc gcaaggtgtt cggccgcgcg cccgaccacg ccgacgacga actgctcgcc
     1681 aacgtcggca aggcgctggc ggcgttcgag gaaacgctcg tgaccgggcg cacgccgttc
     1741 gacgatttcc gtgacgcgct ggaacgcaac gatcaggcgg cgatgcggcg ctatccggcc
     1801 gcggcgcgga gagggctgca gcttttcgtc ggacgcgcca actgcttcgt gtgccacacc
     1861 gggccgaact tcagcaacgg cgaattccac gacgtcggca ttccgttctt cgtggcgaag
     1921 ggcaaggtcg acccgggacg gcacgagggc atccgcatcg tgcaggcgag ccgcttcagc
     1981 ctgctcggga gattcaacga cgacgcgggc ggcgccagcg cgacggcgac gcgccacgtc
     2041 gtgctcgagc atcgccactg gggcgagttc cgcacgccga gtctgcgcaa cgtcgcggtc
     2101 acgccgccgt acatgcacaa cgggtcgctg gcgacgctgc gcgacgtggt gctgcactac
     2161 tcagaactcg atggtgagcg cctgcacgcc gacggcgagc agatcctgcg caagctgaat
     2221 ctgaccgaag aggagatcgg cgacctggtc gcgttcctcg atacgctgac cgacgccaaa
     2281 ggtgagcgcc gactgctgcc gccgatcgat ccgcgagcct gcgagtgatg ccggacggat
     2341 gaatcgttcg atcgcgcgtt cgcgtcgctc atccgaacac ccgtgctgag cgtgactcgc
     2401 ggcccgtgtg cggcggacgt accttgcgcg catggtccgc cgcctcgctc tcgcattgat
     2461 tcccttcgcc ttcgccggtt gcgcgacggt caccgggtcg ccgacgcagt cgatcagcat
     2521 ccacaccgtg gacgccagcg gcaagccggt caacggcatg cgctgccgga tcgccaacgg
     2581 taacgcggac tactacggca actcgccgct gctcgacatg caggtccgcc gctccggcgc
     2641 cgacctggaa atcgaatgcc gcagcggatc gagggtggcg agcgcgaccg cgttcgcgcg
     2701 cagcctgagc ctgaccggcg cggtgctgtt gccgggtggt ggggccgccg cgctgatcga
     2761 ccgcctgagc ggctacgcat acgcctatcc gcgctcgatg caactgcgcg tcggcgagca
     2821 catgatcttc gacgtcagcg ccccggcgcg cgagggcgac gagagcgccg actcgctgcg
     2881 ctag
//
LOCUS       DBNHBY010000070         6814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1695814, whole genome shotgun sequence.
ACCESSION   DBNHBY010000070 DBNHBY010000000
VERSION     DBNHBY010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1695814"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="AC16HB_01670"
     CDS             <1..1183
                     /locus_tag="AC16HB_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5605525.1"
                     /translation="QGAIPPDERVALADEPFDYVVQVWAGDGRITYASRVTPGLPPLA
                     VLGYSEVAIEGRPWRVFSTTARDRVIQVAQPLAVRQSLAAAAAWRSVAPLLVAAPVIA
                     LAVWWLVSVSLAPLRGLVTAVQARDAESLEPLRSAGLPSELAPLVAALNALLARLRTA
                     FDAQRAFVADAAHELRSPLTALKLQLDLARRAPDDATRAQAFGALGAGMERLRHLVEQ
                     LLALARAEPGSAEMALAQTDLAEAARKAAADCVAVAAVRAVDIELQAPHAVIVQGDAS
                     ALRILARNLIDNAVRYAGAGARVVVRVSDAGAGVVLQVDDSGPGIAPHERARVFDRFY
                     RREVDAGNETGGSGLGLAIVQAIAARHDAQVRLADSPLGGLRAEVSFARAAAAATVRA
                     S"
     gene            1183..1782
                     /locus_tag="AC16HB_01675"
     CDS             1183..1782
                     /locus_tag="AC16HB_01675"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5605526.1"
                     /translation="MPPAVQGLIIANVLAFLLESSAGPGLIDALALWVDPAAPLAGVL
                     IAPWQLVTYSFLHGSVAHLALNMLGLWMFGADVERVWGTPRLLLAYFAAVLTGALAQV
                     LAAALFGAAGPVIGASGGVFGLMLAYGLLFPHRRVMLLIPPIPMSARTMVIAYAAVEL
                     VFGITGTAAGVAHFAHLGGLLGGWLAYRYGWRLARGRWR"
     gene            1957..3438
                     /locus_tag="AC16HB_01680"
     CDS             1957..3438
                     /locus_tag="AC16HB_01680"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR5605527.1"
                     /translation="MKLKPLAAVLTAAGLLTAGGAGAWSLKDLLHRPAAAATPATASN
                     QTSATPAVAPAPIAQVAAPNYRAIVQKYGPAVVGVTVEGTRKLAAADMSNLPNDPFFQ
                     FFRGLPGFQFRFGPRGAVPFRGQGSGFIVSSDGVILTNAHVVRDAKEVTVKLSDRHEF
                     RAKVLGADPATDIAALKVDAKNLPTVALGDASQVQVGDYVLAIGAPYGFEETATQGIV
                     SAKGRSLPGDSYVPFIQTDAAVNPGNSGGPLFDAGGRVIGINAQIYSQSGGFQGLAFA
                     IPIDVALHVKDQLISKGHVEHARLGVYLQDLTQSLAESFGMKTPDGALISSVSAGSAA
                     AKASLKPGDVITQIDGEPVHTAGDVSTRIGMAAPGAQARLTVWRDKASRDVTVKLGAA
                     PAADQEQASADGTPGSLGLAVRPLAPEEKRQAHTDHGLVVEEVDGPAARAGIEAGDIV
                     LALNGQPVTSVEQIRRALATRPKHVALLIQRDEQRIFVPINLG"
     gene            3457..3906
                     /locus_tag="AC16HB_01685"
     CDS             3457..3906
                     /locus_tag="AC16HB_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR5605528.1"
                     /translation="MKSIHPTRPTALLLALMLCTGTTAALAATLPAEQHNSGIAYTTG
                     GIGAEQATAFKEAMNRYPLAIELVQKEKSGKRDEFIADAQVRITDHAGKEVFNAKADG
                     PFMLVKLEPGKYSMTASYETHTLHRNDFTVASGRTTHETFVFPADHQ"
     gene            3920..4516
                     /locus_tag="AC16HB_01690"
     CDS             3920..4516
                     /locus_tag="AC16HB_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605529.1"
                     /translation="MKPVLIALGIGAVLAGTTAQAQSVERLLQRNAAQQTRIASAVAL
                     GAIGTHDAAQLEYRAAAVYRVEARALAVPSPDAAARARVHRAEADLASATARAEKHRS
                     VHAGTALDRMHLRVAATRNAEQQRWIVREFRQGTLTASQVAALESAQAKIAHAEFGAA
                     HAGRETLAAAESIQHMQNLQDYAILKDPSLALPAAERA"
     gene            4555..4959
                     /locus_tag="AC16HB_01695"
     CDS             4555..4959
                     /locus_tag="AC16HB_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605530.1"
                     /translation="MRCSSLALGVALCAAARAAAPPTDALRELHSAAHELEQAALLRS
                     VATDATEAHHLLAARDLLREAEPTLHGPLRARAQRLDYDIGRAAGAAAIDVTSPLPDA
                     LGPVSVLPTLDRGELGALARRGLRLARQARTG"
     gene            complement(4871..5884)
                     /locus_tag="AC16HB_01700"
     CDS             complement(4871..5884)
                     /locus_tag="AC16HB_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5605531.1"
                     /translation="MDLAYAMQVFVKVVDGGSFVRASEALDTSNAAVTRQIGALEAHL
                     GARLLNRTTRRLSLTDAGKEFYDRSRAILDDIAEAEAVVGQQSLRPVGLLRITAPLSF
                     GVMQLSRRLPEFRAHYPQLRLDVDLTDRVVDLVNEGVDVAVRIAGELSGNLIARRIAP
                     VSMVVCAAPSYLKRHGTPARPEELSQHETLSFSYLWAGDDWPFVDAHGNTTRVRVQPS
                     VRATNGDLLRELALAGGGIILQPSFLVGADLARGTLVPLLTDFRTLELSLFAVYLSRR
                     HLSVKVRAFVDHLVAAFGAAPWEHWQRTARRSLTPSEPGVRAGGRGAPVRRVRRGRAS
                     AAR"
     gene            6025..6651
                     /locus_tag="AC16HB_01705"
     CDS             6025..6651
                     /locus_tag="AC16HB_01705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5605532.1"
                     /translation="MKVTVIGSGNMGSGFVKQLKAAGHDVRVTGRNADKAKNVAAQFG
                     ATAVSAAEAAASDVLILATGYADAAPALKSLGALDGKIVVDVTNPLTADYMGLTLGHS
                     TSAAEEIARSVPAARVVKAFNTVFAQVLAEGPDFGKNGKAPVFVAGDDEQAKQAVSSL
                     ARSIGFDVIDAGGLKNARYLEPLAGLSIYLGYGAGFGTNIAPTWIRRG"
BASE COUNT         1056 a         2434 c         2346 g          978 t
ORIGIN      
        1 ccagggcgca atcccgccgg acgagcgcgt ggcgctcgcc gacgaaccct tcgactacgt
       61 cgtgcaggtc tgggcgggcg acggcaggat cacctacgca tcgcgcgtca cgccggggct
      121 gccgccgctc gcggtgctcg gctattccga ggtcgcaatc gagggccggc cctggcgcgt
      181 tttcagcacg accgcgcgcg accgcgtgat ccaggtcgcg cagccgctcg cggtgcgcca
      241 gtcgctcgcg gcggcggccg catggcggag cgtggcgccg ctgctcgtgg ccgcaccggt
      301 catcgcgctg gccgtgtggt ggctggtcag cgtgtcgctg gcgccgctgc gaggcctcgt
      361 gacggccgtg caggcgcgcg acgccgagtc gctcgagccg ctgcgaagcg ccggactgcc
      421 gtccgagctc gcgccgctgg tggccgcgct gaacgcgctg ctggcgcgcc tgcgcaccgc
      481 gttcgacgcg cagcgcgcgt tcgtcgccga tgccgcgcat gagctgcgct cgccgctgac
      541 ggcgctgaag ctgcagctcg atcttgcgcg ccgcgcgccg gacgacgcca cgcgcgcgca
      601 ggcgttcggc gcgctcggcg ccggcatgga gcgcctgcgc catctcgtcg agcaattgct
      661 ggcgctggcg cgcgccgaac ccggcagcgc cgagatggcg ctcgcacaga ccgatctggc
      721 cgaagccgcg cgaaaggcag ctgcagactg cgttgccgtc gccgccgtgc gcgcggtcga
      781 catcgaactg caggcgccgc acgcggtgat cgtgcaaggg gatgcttcgg cgttgcggat
      841 tctggcgcgc aatctgatcg acaacgcggt gcgctatgcg ggcgccggcg cgcgcgtcgt
      901 cgtgcgcgtg tcggacgccg gcgcaggtgt ggtgctgcag gtcgacgact ccgggccggg
      961 cattgcgccg catgaacgcg cgcgtgtgtt cgaccgcttc tatcgccgcg aggtcgacgc
     1021 cggcaacgag acgggtggca gcggcctggg cctggcgatc gtgcaggcga tcgccgcgcg
     1081 ccatgacgcg caggtgcggc tggccgactc gccgctcggc ggactgcgcg ccgaggtgag
     1141 ctttgcgcgc gcggccgcgg ccgccacggt acgggcgtcg tgatgccgcc ggcagtccag
     1201 ggcctgatca tcgccaacgt gctcgcgttc ctgctcgaat cgagcgcggg accgggcctc
     1261 atcgacgcgc tcgcgctgtg ggtcgatccg gccgcaccgc ttgccggcgt gctgatcgcg
     1321 ccgtggcagc tcgtcaccta cagctttctg cacggcagcg tcgcgcatct ggcgctcaac
     1381 atgctcgggt tgtggatgtt cggcgccgac gtcgagcgcg tatggggcac gccgcgattg
     1441 ctcctcgcgt acttcgccgc cgtgctcacc ggcgcgctgg cacaagtgct ggccgccgcc
     1501 ctcttcggcg cagcggggcc ggtgatcggt gcctcgggcg gcgtgttcgg actgatgctc
     1561 gcctatgggt tgttgttccc gcaccggcgc gtgatgctgc tgattccgcc aattccgatg
     1621 tcggcgcgca cgatggtgat cgcgtatgca gcggtcgaac tcgtgttcgg gatcaccggc
     1681 accgcggccg gagtcgcgca tttcgcgcac ctcggcggac tgctcggcgg ctggctcgcg
     1741 tatcgctatg gctggcgcct cgcgcgcggg cgctggcgct gagcggcgta cggatgttcg
     1801 acgcgtcgca ctgtcgtgag cgaccgttcc cacacgatgc cggcgctccg cacgctccta
     1861 atcttcatct aacgcagcat taaccgtcgg ctcaccgcgc gctcctagag ttccgatcga
     1921 gcgcaagaca ctgcgcgatc ggagaaagga accgcgatga aactcaagcc ccttgccgcc
     1981 gtgctgaccg cagcgggcct cctgacggcc ggcggcgccg gcgcgtggag cctcaaggat
     2041 ctgctgcatc gaccggccgc cgcggcgacg ccggcgaccg catcgaacca aaccagtgcg
     2101 accccggccg tcgccccggc gccgatcgcg caggtggccg cgcccaacta tcgcgccatc
     2161 gtgcagaagt acggccccgc ggtggtcggt gtgaccgtcg agggcacgcg caagctcgcg
     2221 gccgccgaca tgtcgaatct gccgaacgat cccttcttcc agttcttccg cggcctgccg
     2281 ggattccagt tccgcttcgg accccgcggg gcggtgccgt tccgcggcca gggctcgggt
     2341 ttcatcgtca gttccgacgg cgtcatcctg accaacgcgc acgtggtgcg cgacgccaag
     2401 gaggtgactg tcaagctgtc tgaccggcac gagtttcgcg ccaaggtgct cggcgcggac
     2461 ccggccaccg acatcgcggc gctgaaggtc gacgcgaaga acctgccgac cgtcgcgctc
     2521 ggcgacgcca gtcaggtgca ggtcggcgac tacgtgctgg caatcggcgc cccttacgga
     2581 ttcgaggaga ccgccacaca gggcatcgtc agcgccaagg gccggtcgct gcccggcgat
     2641 tcttacgtgc ccttcatcca gaccgatgcc gcggtcaacc cgggcaactc cggcggaccg
     2701 ctgttcgacg cgggcggccg cgtgatcggc atcaacgcgc agatctactc gcaaagcggc
     2761 ggtttccagg ggctggcgtt cgcgatcccg atcgacgtcg cgctgcacgt gaaggaccag
     2821 ttgatcagca agggccacgt cgagcatgcg cgcctgggcg tgtacctgca ggacctgacg
     2881 caatccctcg ccgagtcgtt cgggatgaag acgccggacg gcgcgctgat ctccagcgtg
     2941 tcagccggca gcgccgcggc caaggcgagc ctgaaaccgg gtgacgtgat cacgcagatc
     3001 gacggcgaac ccgtccatac ggccggcgac gtgtcgacgc gcatcggcat ggcggccccc
     3061 ggcgcgcagg cgcgcctgac ggtctggcgc gacaaggcaa gccgcgatgt gaccgtgaag
     3121 ctcggcgccg cgccggcggc ggatcaggag caggcgtccg ccgacggcac gccgggttcg
     3181 ctcgggctcg ccgttcggcc gctggcgccc gaggaaaagc gccaggcgca caccgatcac
     3241 gggctcgtcg tcgaagaagt cgacggcccg gcggcgcgcg ccggcatcga ggcgggcgac
     3301 atcgtgctcg cgctgaacgg ccagcccgtc accagcgtcg agcagatccg ccgcgcactc
     3361 gccacccgtc cgaagcacgt agcgctgctg atccagcgcg acgagcagcg gatcttcgtg
     3421 ccgatcaacc tcggctgaac gtcaaaggag gacgcgatga aatcgattca cccgacccga
     3481 cccaccgcac tgctgcttgc gctgatgctc tgtaccggca cgacggccgc gctcgcggcc
     3541 actctgccgg ccgagcagca caactccggc atcgcctata cgaccggcgg catcggagcc
     3601 gagcaggcga cggccttcaa ggaagcgatg aacaggtatc cgctcgcgat cgaactggtg
     3661 cagaaggaaa agagcggcaa gcgcgacgag ttcatcgccg acgcgcaggt ccgcatcacc
     3721 gatcacgccg gcaaggaagt gttcaacgcg aaggccgacg gcccgttcat gctggtgaag
     3781 ctcgaacccg gcaagtattc gatgaccgcc agctacgaga cgcacacgct gcaccggaac
     3841 gacttcaccg tggccagtgg caggaccacg cacgagacgt tcgtcttccc ggcggaccac
     3901 cagtagacgg agcggcacca tgaaacctgt tctgatcgca ttgggcatcg gcgccgtgct
     3961 ggccggcacg accgcgcagg cgcagagcgt cgagcggctg ctccaacgca acgccgccca
     4021 gcagacgcgc atcgccagcg ccgtcgcgct tggcgcaatc ggcacgcacg acgccgcgca
     4081 actcgaatac cgcgccgccg cggtctaccg cgtcgaagcg cgggcactcg cggtgccgag
     4141 ccccgacgcc gcagcgcgtg cgcgcgtgca ccgggccgag gccgatctcg cgagcgcaac
     4201 tgcacgggcc gagaagcatc ggtctgtgca cgccggcacc gcactcgacc gcatgcatct
     4261 gcgcgtggcg gcaacgcgca acgcagagca gcagcgctgg atcgtccgcg aattccggca
     4321 gggcacgctg acggcgagcc aggtcgccgc gctcgaatcc gcccaggcga agatcgcgca
     4381 cgccgagttc ggcgccgcgc acgcggggcg cgagacgctt gccgcggccg aatcgatcca
     4441 gcacatgcag aacctgcagg actacgcgat cctgaaggat ccgtcgctcg ccctgccggc
     4501 cgccgagcgc gcgtgaagcc gcgtcggtgc gaaggttgtc cgatgacggc ccgcatgcgc
     4561 tgttcgtccc tcgcgctcgg cgtcgcgctg tgcgcggccg cgcgcgccgc ggcgccgccg
     4621 accgatgcgc tgcgcgaatt gcactcggct gcgcatgaac tcgagcaggc ggcgctgctg
     4681 cgctccgtcg ccaccgacgc gaccgaggcg catcatctgc tcgccgcgcg cgacctgctg
     4741 cgcgaggccg agccgacgct gcacggcccg ctgcgcgcgc gggcgcaacg ccttgactac
     4801 gatatcggcc gcgctgccgg tgccgcagca atcgacgtca cctcgccgct gcccgatgcg
     4861 ctcggccccg tcagcgtgct gccgacgctc gaccgcggcg aactcggcgc actggcgcgc
     4921 cgcggcctcc ggctcgcacg ccaggctcgg acggggtgag cgatcgccgc gccgtgcgct
     4981 gccaatgctc ccacggcgcc gcgccgaacg ccgcgacaag gtgatcgacg aatgcgcgca
     5041 ccttgaccga caggtgccgc cggctcagat agaccgcgaa caggctcagt tcgagcgtcc
     5101 tgaaatccgt caacagcggc acgagcgtcc cgcgcgccag gtcggcgccc accagaaagc
     5161 tcggctgcag aatgatgccg ccgccggcca gggcgagttc gcgcagcaga tcgccgttgg
     5221 tcgcgcgcac gctcggttgc acacgcacgc gcgtcgtgtt gccatgtgca tcgacaaacg
     5281 gccagtcgtc gccggcccac aggtaactga agctcagcgt ctcgtgctgc gacagctctt
     5341 cgggccgcgc gggcgtgccg tggcgcttca ggtatgacgg cgctgcgcac accaccatgc
     5401 tgaccggcgc aatgcggcgc gcgatcaggt tgccgctcag ttcgcccgcg atgcgcaccg
     5461 ccacgtcgac gccttcgttg acgagatcga ccacgcggtc ggtgagatcg acgtccagcc
     5521 gcagttgcgg atagtgcgcg cgaaattccg gcagccgccg cgacaactgc atcacgccga
     5581 acgacagcgg cgcggtgatg cgcagcagcc cgaccggccg cagcgactgc tgtcccacga
     5641 ccgcttcggc ctcggcaatg tcgtcgagga tggcgcggct gcggtcgtag aactccttgc
     5701 cggcgtcggt cagcgacagc cggcgcgtcg tgcgattgag cagccgcgcg cccaggtgcg
     5761 cctcgagcgc gccgatctgg cgcgtcacgg cggcgttcga cgtatcgagc gcctccgacg
     5821 cgcgcacgaa actgccgccg tcgaccacct tcacgaacac ctgcatcgca tacgccaggt
     5881 ccatcgccgc ccccatgaat cattacgatc atcgtaatgg tcaattccgg ccagcacgct
     5941 gtttcccggc cgccgcgact cgtacagtgc atgcatcgag gcagcacacc gattcacaga
     6001 tccgcacacc tgcaaggagg tcaaatgaaa gtgacagtca tcggctcggg caacatgggc
     6061 tcgggcttcg tcaagcaact gaaggccgcg ggccacgacg tccgcgtcac gggccgcaat
     6121 gccgacaagg ccaagaacgt cgcggcgcag ttcggcgcca ccgcggtcag tgccgcggag
     6181 gcggcggcca gcgacgtgct gatccttgcc accggctatg ccgatgcagc gccggcgctg
     6241 aagtcgctcg gcgcgctgga cggcaagatc gtcgtggacg tcaccaaccc gctgaccgcc
     6301 gattacatgg gactgacgct cggccacagc acgtcggccg cggaggaaat cgccaggtcc
     6361 gtccccgctg cgcgcgtcgt caaggccttc aacaccgtgt tcgcacaggt gctcgccgag
     6421 ggccccgact tcggcaagaa cggcaaggcg ccggtgtttg tcgctggtga cgacgagcag
     6481 gccaagcagg ccgtctcatc gctggcccgt tcgatcggct tcgacgtgat cgatgccggg
     6541 ggcctgaaga acgcacgcta tctggaaccg ctggcgggtc tgagcatcta cctcggctac
     6601 ggcgccgggt tcggcaccaa catcgcgccg acctggatcc gccgcgggtg aaggcaggaa
     6661 agcggcatag ccggcaacgc ttcgcgaagg attcgaagcc atcctgcgcc ggacggcgcc
     6721 ggcgccgata gcgtcgacgg tcgttgcgac gttcgtgctt cacgcgttcc gggccgcgcc
     6781 caccgcgccg gcaatgggcg gcggatcgac ttca
//
LOCUS       DBNHBY010000071         2167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_274476, whole genome shotgun sequence.
ACCESSION   DBNHBY010000071 DBNHBY010000000
VERSION     DBNHBY010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_274476"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC16HB_01710"
     CDS             <1..561
                     /locus_tag="AC16HB_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605533.1"
                     /translation="HRNVRLDPVPLAPAPPADVQRPADVGLRTFDELNATMSELTGIA
                     TTEPGVAATYATIRQSLPTVETIDTFVSAHPVAVAQLSIQYCNALVENPTARAAYFPG
                     FDFAAAPATAFAPAGRAIVLDALVGRMLRANVGTEPDAAGVRGDLDALITRLTACAGS
                     CPAGRTATVVKASCAALLGSAATLIQ"
     gene            589..>2167
                     /locus_tag="AC16HB_01715"
     CDS             589..>2167
                     /locus_tag="AC16HB_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605534.1"
                     /translation="MLIRHKPPRTLAPDEPLRHADAHKRPVTRRDFIAQGLSRGLATV
                     VAPGVFSLFANPRAAEAALSSDLQALKASCGIDVQGAGKIPFICIDLAGGANMVGSNV
                     LIGGPGGQLDFLSTAGYGKLGLPGDMLPGMTNTTTGTSFTDTRLGLAFHSDSAFLRGI
                     LDRVAPATAAAINGAVIAARSENDTGNNPHNPMYGIHQAGADGSLATLIGSRASDSGG
                     NSMAPAGMIDLSVRPTKVDRASDVTGLVDTGQLASLLSLDDTVAVMEAIQRISDRKLK
                     VIDPKQPWMDPKRPTVTRNEVVRELVSCGYVKAADVVDRYGDPASLNPDLDPDIVGAG
                     GIFSRAEYDADAEFRKTAAVMKMVLSGYAGAGTITLGGYDYHGQGRATGELRDLRAGR
                     CMGACLEYAARRGVPLMLYVYSDGSISASGQVDNSADGRGKFMWTSDNQSTAASFFLV
                     YNPCGRATLVSTGPGGAARHQQIGYFRADASVETASSPAANNVNLLVETVVLNYMALH
                     GEQGQFGTRFPRNGLGSA"
BASE COUNT          339 a          790 c          743 g          295 t
ORIGIN      
        1 caccgcaacg tgcgcctcga cccggtgccg ctcgcgccgg cgccgcccgc cgacgtgcag
       61 cgtcccgcgg acgtgggcct gcgcaccttc gacgaactga acgcgacgat gtccgaactg
      121 accggcatcg ccaccacgga gccgggcgtc gccgcaacgt acgcgaccat tcgccagtcg
      181 ttgccgaccg tcgagacgat cgacacgttc gtgtctgcgc atccggtcgc cgtcgcgcag
      241 ctgtcgatcc agtactgcaa cgcgctggtg gagaacccca ccgcgcgcgc cgcgtacttc
      301 ccgggattcg acttcgcggc ggcgccagcc acggcgttcg cgccggcggg acgcgccatc
      361 gtgctcgacg cgctggtcgg tcgcatgctg cgcgccaacg tcggcaccga gccggatgcc
      421 gcaggcgtgc gcggcgacct cgacgcgctg atcacgcggc tcacggcctg cgccggcagc
      481 tgtccggcgg gtcgcaccgc gacggtcgtg aaggcgagct gtgccgcgct gctcggcagc
      541 gccgcgacgc tgatccaatg acggcccgat gacgacgagg acgtacgcat gctgatccgt
      601 cacaagccgc cgcgcacgct cgctccggac gagccgctgc gacacgccga cgcgcacaag
      661 cggccggtca cgcgccgcga cttcatcgcg cagggcctga gccgcgggct cgccaccgtc
      721 gtcgcaccag gtgtcttcag cctgtttgcc aacccgcgtg cggccgaggc ggcgctgtcg
      781 tcggacctgc aggcactcaa ggcgagctgc ggcatcgacg tgcagggcgc cggcaagatc
      841 ccgttcatct gcatcgacct cgccggcggc gccaacatgg tcggctccaa cgtgctgatc
      901 ggcggaccgg gcggccagct cgatttcctg tccacggcgg gttacggcaa gctcgggctg
      961 ccgggcgaca tgctgccggg catgaccaac acgaccaccg gcaccagttt caccgacacg
     1021 cggctcggcc tggcgttcca cagcgacagc gcgttcctgc gcggcatcct cgaccgcgtg
     1081 gcgccggcca cggccgccgc gatcaacggt gcggtcatcg cagcgcgctc cgagaacgac
     1141 accggcaaca acccgcacaa cccgatgtac ggcatccacc aggcgggcgc cgacggttcg
     1201 ctggcgacgc tgatcggctc gcgcgcgtcc gactcgggcg gcaactcgat ggcgccggcc
     1261 ggcatgatcg acctctccgt gcggccgacg aaggtcgacc gggcgagcga cgtgaccggc
     1321 ctcgtggaca cgggccagct cgcgagcctg ctgagcctcg acgacaccgt cgcggtcatg
     1381 gaggcgatcc agcgcatcag cgaccgcaag ctcaaggtca tcgacccgaa gcagccgtgg
     1441 atggacccca agcggccgac ggtgacgcgc aacgaagtcg tgcgcgaact cgtcagctgc
     1501 ggctacgtca aggcggccga cgtggtcgac cgctatggcg atcccgcgag cctcaatccg
     1561 gacctcgacc cggacatcgt cggcgcgggc ggcatcttca gtcgcgccga atacgacgcc
     1621 gacgccgagt tccgcaagac ggccgcagtg atgaagatgg tgctgtccgg ttatgccggc
     1681 gccggcacga tcacgctcgg cggctatgac taccatggcc agggtcgtgc gaccggcgag
     1741 ctgcgcgacc tgcgcgcggg ccgctgcatg ggcgcgtgcc tggagtacgc cgcgcgccgc
     1801 ggcgtgccgc tgatgctgta cgtgtactcg gacggctcga tctcggcgag cggccaggtc
     1861 gacaacagcg ccgacgggcg cggcaagttc atgtggacca gcgacaacca gtccacggcc
     1921 gcgtcgttct tcctcgttta caacccgtgc ggccgcgcaa cgctggtgtc gaccgggccg
     1981 ggcggcgccg cacgacatca gcagatcggg tatttccgcg cggacgcgtc ggtggagacc
     2041 gcctcgagtc cggccgcgaa caacgtgaac ctgctggtcg aaacggtcgt gctgaactac
     2101 atggcgctgc acggcgagca ggggcagttc ggcacgcgct tcccgcgcaa cgggctcggc
     2161 agcgcgt
//
LOCUS       DBNHBY010000072         5147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1831197, whole genome shotgun sequence.
ACCESSION   DBNHBY010000072 DBNHBY010000000
VERSION     DBNHBY010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1831197"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16HB_01720"
     CDS             <1..311
                     /locus_tag="AC16HB_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HIR5605535.1"
                     /translation="RAAIVRFLQRKWARELDYRLIKELWAFGENRIAVRFAYEWHDDS
                     GTRFRSYGNENWEFDERGLMRRRIASINDLPITAADRKYRWPLGRRPDDHPGLSDLGL
                     "
     gene            complement(281..1615)
                     /locus_tag="AC16HB_01725"
     CDS             complement(281..1615)
                     /locus_tag="AC16HB_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HIR5605536.1"
                     /translation="MLLLLLSYVAGALTIVAPCILPVLPFVFARADRSFARNGLPLLM
                     GLALGFTAVATLATVAGGWAASVNEAGRGVALAALALTGLTMLVPRLADLWAQPLVAL
                     GARLGDVAQRTAPPDGRPLWASLALGVATGLLWAPCAGPVLGLVLSTAALQGPSVHTA
                     LLLFAYAAGAATSLALALLVGGRVFAAMRRALGAARWLRQALGLAVLAGVTAIALGLD
                     TGLLAELSSSQTDALEQKLLQRWRQGVTFREDAAAGAHLQRAVLPVERARPVPAALPA
                     LNLPVEGNLPSLAGATDWLNAQPITAESLRGKVVLIDFWTFGCINCRHALPYVRAWAD
                     KYRDQGLVVIGVHSPEFAYERKLDNVKRAARDLGLTFPIAIDNDFAIWRAFANEYWPA
                     HYFVDAQGRIRFHKFGEGDYERSEQVIRQLLREAGAARLSARDYSPRSDSPG"
     gene            complement(1637..1960)
                     /locus_tag="AC16HB_01730"
     CDS             complement(1637..1960)
                     /locus_tag="AC16HB_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HIR5605537.1"
                     /translation="MNKLAAALLSVSMVFVAGSALAQEAMKHDSMAKDAMEKDAMKKD
                     AMGKDAMHKDTMKKDAMHRDAMAKDSMKKDSMKNDAMKKDAMHHDAMQKDSGGDAMAK
                     DAMKK"
     gene            complement(2034..2825)
                     /locus_tag="AC16HB_01735"
     CDS             complement(2034..2825)
                     /locus_tag="AC16HB_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5605538.1"
                     /translation="MKIFERPRATGVDGTAAVREAARLLASRAGSPARRAFLQRSLSL
                     GGLALLTGCSITDDEGVERALAAISRANDRVQAWLFDPARLAPTYEDALIARPFPFNA
                     YYGESEVPQIDGSGWTLEVAGLVADRRRWTLADLRALPQIEQTTRHICVEGWSAIGKW
                     GGVPFAQFLRRVGADLTAKYVGFKCADDYYTSIDMPTALHPQTILALTFDGGALPPKH
                     GFPVKLRIPTKLGYKNPKHIQTIFVTNTYPGGYWEDQGYNWFGGS"
     gene            complement(2822..3466)
                     /locus_tag="AC16HB_01740"
     CDS             complement(2822..3466)
                     /locus_tag="AC16HB_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5605539.1"
                     /translation="MHSLPATDTATAASFTPAIHPPWLRAAHWLNALAVLLMVASGWR
                     IYDAAPLFDLRFPRELTLGGWLGGALQWHFAAMWLLGANGLFYLTLNLATGRFRRTFL
                     TLRLSDIWHDFAAALRSRLSHGDSRKYNAVQRAAYLFAVADLVLLVLSGLVLWKSVQF
                     PYLRLVLGGYEGARRVHFFAMAALVGFVAIHLTMVVLVPRSLLAMLGWHRGVSS"
     gene            3594..4301
                     /locus_tag="AC16HB_01745"
     CDS             3594..4301
                     /locus_tag="AC16HB_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5605540.1"
                     /translation="MPKRVLIVEDDDNIAELMRMHLHDEGYAIEHTADGHAGLALVER
                     EHWDALVLDLMLPGVDGLEICRRARSMARYTPIIITSARSSEVHRILGLELGADDYLA
                     KPFSLLELVARMRALLRRADALARNARLEAGHVEVGELWIDPVAREARVRDAPIELTP
                     REFDLLHFFARHPGKVFSRLELLNRVWGYRHEGYEHTVNTHINRLRVKIESDPANPRR
                     ILTVWGRGYKLAADGDA"
     gene            4298..>5147
                     /locus_tag="AC16HB_01750"
     CDS             4298..>5147
                     /locus_tag="AC16HB_01750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5605541.1"
                     /translation="MIGRLSLSQRLGAVFAVLLLACCGAAAWLQLRTNERHDQVVAQR
                     LSGGLAAHIAGSAALMEHDGLNQPAVRELFQKLMDVNPSVEVYLLAPDGRIVAQAAPA
                     GHLRRDRVDVAPIRRLLAGGELPIFGDDPRNATAQKVFNAAPLRVDGRDAGYVYVVLH
                     GEQLDTLAAQVGDDDVMRTALASLALVALLALVAGLAAFHWITRPLRELTADVRRFGA
                     DASHAVAPPAVPARIAHGRGELATLRMAFAQMTQRIGEQWHELTLQDQQRRELVANIS
                     HDLRTPL"
BASE COUNT          876 a         1753 c         1737 g          781 t
ORIGIN      
        1 gacgggcagc gatcgtgcgg ttcctgcagc gcaagtgggc gcgggaactc gattaccgcc
       61 tgatcaagga actgtgggcg ttcggtgaga accgcatcgc cgtgcgcttc gcctacgagt
      121 ggcacgacga ctcgggtact cggttccgct catacggcaa cgagaactgg gagttcgacg
      181 agcgtgggct gatgcggcgc cggattgcca gcatcaacga cctcccgatc acggcagcgg
      241 accgcaagta ccgctggccg ctcgggcggc gcccggatga tcaccccgga ctgtcggacc
      301 tcgggctgta gtcgcgggcg ctcagccgtg ccgctccggc ctcccgcagc aactgccgga
      361 tcacctgctc ggaccgctcg tagtcgcctt cgccgaactt gtggaagcgg atgcgcccct
      421 gggcgtcgac gaagtagtgc gccggccagt attcgttggc aaaagcgcgc cagatcgcga
      481 agtcgttgtc gatcgcgatc gggaaggtca ggccgagatc gcgcgccgcc cgcttcacgt
      541 tgtcgagctt gcgctcgtag gcgaattcgg gcgaatgcac gccgatgacg acgaggccct
      601 ggtcgcgata cttatccgcc cacgcccgca cgtacggcag cgcatggcgg cagttgatgc
      661 agccgaaggt ccagaaatcg atcagcacga ccttgccgcg cagcgattcg gcggtgatgg
      721 gctgcgcatt gagccagtcg gtggcaccgg cgagcgacgg cagattgcct tcgaccggca
      781 aattcaatgc gggcaacgcc gccggcacgg gtcgcgcgcg ctcgacgggc agcaccgcgc
      841 gctgcagatg cgcgccggcg gcagcgtcct cgcggaaagt cacgccctgg cgccagcgtt
      901 gcagcaactt ctgctcgagc gcatcggttt ggctcgacga cagttcggcc agcagaccgg
      961 tatccagccc gagcgcgatc gccgtcacgc cggccagcac cgcaaggccc agcgcctggc
     1021 gcagccatcg agcggcgccg agcgcgcggc gcatcgccgc gaacacgcgg ccgccgacca
     1081 gcagcgcgag cgcaagcgat gtcgccgcgc cggccgcata agcgaacagc agcagcgccg
     1141 tatgcacgct cggcccctgc agtgccgccg tcgacaacac cagaccgagc accgggccgg
     1201 cgcaaggtgc ccatagcaat ccggtggcga cgccgagcgc gagcgacgcc cacagtggtc
     1261 ggccatcggg cggggccgtg cgctgggcaa cgtcgccgag gcgtgcgccg agcgccacga
     1321 gcggctgcgc ccacagatcg gcgagccgcg gcaccagcat cgtgagaccg gtcagcgcca
     1381 gcgcggccag tgcaacgccg cggcccgcct cgttgacgct cgctgcccag ccgccggcca
     1441 ccgtggccaa cgtcgcgact gcagtgaacc ccaaggcgag gcccatcagc agcggcagcc
     1501 cattgcgcgc gaaggaccgg tccgcccgtg cgaagacgaa tggcagcacg ggcaggatgc
     1561 agggggcgac gatggtcaag gcgccggcga cgtaggacaa caagagaagc agcacgatgg
     1621 gactcctgcg caggaactac ttcttcatcg cgtccttcgc catcgcgtcg ccgccggaat
     1681 ccttctgcat cgcgtcatgg tgcatggcgt ctttcttcat tgcatcgttc ttcatcgagt
     1741 ccttcttcat gctgtccttc gccatcgcat ccctgtgcat ggcgtccttc ttcatggtgt
     1801 ccttgtgcat cgcgtccttg cccatcgcgt ccttcttcat ggcgtccttt tccatggcgt
     1861 ccttggccat cgagtcgtgt ttcatcgctt cctgcgcgag cgcactcccg gcgacgaaca
     1921 ccatggagac actcagaagt gcggcggcca gcttgttcat gatcgtttcc tttcgttgac
     1981 tgagtagaga gtggacggca acagtgccga gttgtcggga caggcgagcg gactcagctg
     2041 ccgccgaacc agttgtatcc ctggtcttcc cagtaaccgc cgggatacgt gttcgtgacg
     2101 aagatcgtct ggatgtgctt cggattcttg tagccgagct tggtcggaat ccgcagcttc
     2161 acgggaaagc cgtgcttcgg cggcagcgct ccgccgtcga aggtcagcgc gagaatcgtc
     2221 tgcggatgca gcgcggtcgg catgtcgatg ctggtgtagt agtcgtcggc acacttgaag
     2281 ccgacgtatt tcgcggtgag gtccgcgccc acgcgccgca ggaattgcgc aaagggcacg
     2341 ccgccccact tgccgatcgc gctccagccc tcgacgcaga tgtggcgcgt ggtctgctcg
     2401 atctgcggca acgcgcgcag gtcggcaagc gtccagcgcc ggcgatcagc gaccaggccg
     2461 gcaacttcga gcgtccagcc ggacccgtcg atctgcggaa cttcgctctc gccgtaatag
     2521 gcattgaagg ggaacggccg cgcgatcagc gcatcctcgt atgtcggcgc caggcgcgcg
     2581 ggatcgaaca gccacgcctg cacgcgatcg ttggcgcgcg agatcgccgc cagcgcgcgc
     2641 tccacgcctt cgtcatcggt gatgctgcag ccggtgagca gcgcaagccc gccgagcgac
     2701 agcgagcgct gcaggaaggc acggcgcgcc ggtgaacccg cgcgcgatgc cagcaaacgc
     2761 gccgcttcgc gcacggcggc ggtgccgtcg acgcccgttg cgcgtggtcg ctcgaagatc
     2821 ttcatgagga cactcctctg tgccagccga gcatcgccag cagcgagcgc ggcaccagta
     2881 ccaccatcgt cagatggatc gcgacgaaac cgaccagcgc cgccatcgcg aagaagtgca
     2941 cgcggcgcgc gccttcgtac ccacccagga cgaggcgcag ataggggaac tgcaccgact
     3001 tccaaagcac gaggcccgaa agaaccagca gcaccaggtc ggcaaccgcg aacaggtaag
     3061 cggcgcgctg tacggcgttg tacttacgcg agtctccgtg cgagaggcgg cttcggagag
     3121 cggcggcgaa gtcatgccag atgtcgctca ggcgcagcgt aagaaacgtg cggcggaatc
     3181 gaccggtcgc gaggttcagc gtcaggtaga agagcccgtt ggcgcccagc agccacatcg
     3241 cggcgaagtg ccactgcagc gcgccgccca gccagccgcc caatgtcagc tcgcgcggga
     3301 aacgaagatc gaacaagggc gcggcgtcgt agatgcgcca gccgctggcg accatcagca
     3361 gcacggccag cgcattgagc cagtgcgccg cgcgcagcca gggcggatgg atcgccggcg
     3421 tgaacgatgc ggcggttgcg gtgtctgttg cagggagcga atgcatggcg ccagtctggc
     3481 cgcggacgca tcgccaagtc ctcccggcga gttaaacaat ttgtgaacat cgacctcgcg
     3541 attcgatgga acaatcgcat cgcagtcgca gcgcgcagcc tcgagagtcg cccatgccca
     3601 aacgcgtcct gatcgtcgaa gacgacgaca acatcgccga gctgatgcgc atgcacttgc
     3661 acgacgaggg ctatgcgatc gagcacacgg ccgacggcca tgcggggctc gccctcgtcg
     3721 agcgcgagca ctgggacgcg ctcgtgctcg acctgatgct gccgggcgtg gacggactgg
     3781 agatctgccg ccgcgcccgc tcgatggcgc gctacacccc catcatcatc accagcgcgc
     3841 gctcgagtga agtgcatcgc atcctcggcc tcgagctcgg cgccgacgat tacctggcca
     3901 agccgttctc gctgctcgaa ctcgtcgcgc gcatgcgcgc attgctgcgg cgtgccgacg
     3961 ccctggcccg caatgcgcgg ctggaagccg gccacgtcga ggtcggcgaa ctctggatcg
     4021 atccggtggc gcgcgaagcg cgcgtgcgcg acgcaccgat cgagctgacg ccgcgcgagt
     4081 tcgacctgct gcatttcttc gcccgccatc cgggcaaggt gttctcgcgg ctcgaactgc
     4141 tgaaccgcgt ctggggctac cggcacgagg gctacgagca cacggtcaac acgcacatca
     4201 accggctgcg cgtgaagatc gaaagcgacc cggcgaatcc gcgccgcatc ctgaccgtgt
     4261 gggggcgcgg ctacaagctc gccgcggacg gcgacgcatg atcgggcggc tgtcgctgtc
     4321 gcagcgcctg ggcgcggtgt tcgcggtgct gctgctcgct tgctgcggcg ccgccgcctg
     4381 gctgcaactg cgaacgaacg agcgccacga tcaggtggtc gcccagcgac tgtcgggcgg
     4441 cctggccgcg cacattgccg gcagcgccgc gctgatggaa cacgacggcc tgaatcagcc
     4501 ggccgtgcgc gagctgttcc agaagctgat ggacgtgaac ccgagcgtcg aggtgtacct
     4561 gctggcgccc gacggccgca tcgtggcgca ggccgcgccc gcaggtcatc tgcggcgcga
     4621 ccgcgtcgat gtcgcgccga ttcgtcgcct gctcgccggc ggcgaactgc cgatcttcgg
     4681 cgacgacccg cgcaacgcca ccgcgcagaa ggtcttcaac gcggcaccgc tgcgtgtcga
     4741 tggccgcgat gcgggttacg tctacgtggt gctgcacggc gaacagctcg acacgctggc
     4801 ggcgcaggtc ggcgacgacg acgtgatgcg cacggcgctc gcttcgctgg cgctggtggc
     4861 gctgctcgcg ctggtggccg ggctcgcggc ttttcactgg atcacgcgtc cgctgcggga
     4921 actcaccgcg gatgtgcgcc gcttcggcgc cgacgcgtcg cacgccgtcg cgccgcccgc
     4981 ggtgccggcg cgcatcgcgc acggccgcgg cgaactggcg acgctgcgca tggccttcgc
     5041 gcagatgacc cagcggatcg gcgagcagtg gcatgaactg acactgcagg accagcagcg
     5101 gcgcgaactg gtggccaaca tctcgcacga cttgcgcacg ccactga
//
LOCUS       DBNHBY010000073         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_883709, whole genome shotgun sequence.
ACCESSION   DBNHBY010000073 DBNHBY010000000
VERSION     DBNHBY010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_883709"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="AC16HB_01755"
     CDS             <1..1733
                     /locus_tag="AC16HB_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605542.1"
                     /translation="FNSVAVRLARGAAEPGVIAALDRLLDRYGFTGAYGRTDQLSHKA
                     LTQEISQWRVYGTALPVVVLGVAVFLLNVALTRQIGTQRSQIAALKALGCDDRAIGLH
                     YLKFVLVIVLIGGPIGVAGGYYFGYAVAQLYARFFRFPDFEFRMLAWIPLSAVLLSMF
                     AAIAGVVTALRRVVRLPPAEAMRPAAPATFRPTLMEAIGLGHLYSPAVRMVVRDIERK
                     PLRAILTTFGIATAVAVMIAGTWWRDAVDYLLDVEIRMRDRQEVSIALTEPTSASAIY
                     ELARLPGVLRAEPDRDVAVRLSSGPRTYRTSLSGLRADGQMRPLLDARLQRIALPSAG
                     IVLNRRLAERLDLKLGDPVRVEVLQGTRAEATLTVSGFSHELMQLPAYIDYDALNRLL
                     GEGDALSGARLLIDRTQRAALFAQIKQTPRAAVAVEIGPIIRNVRENTARNILFFTSV
                     MAVMGAAIAFGVVYNNARIALAERAWDLASLRVLGFTRGEVSGLLLGELAVELAVALP
                     LGAVLGHWLSWGILQMMQHETMQLPFVIEPGTYAFACLVVLIAGAASALVVRQRIDHL
                     DLVAVLKTRE"
BASE COUNT          242 a          583 c          655 g          307 t
ORIGIN      
        1 ccttcaactc ggtggcggtc cgattggcgc gcggcgccgc ggagccgggc gtgatcgcgg
       61 cgctcgaccg cctgctcgac cgctacggct tcacgggtgc atacggtcgc accgatcagc
      121 tttcgcacaa ggcgctgacc caggagatct cgcaatggcg ggtctacggc acggcgctgc
      181 cggtcgtcgt cctcggcgtg gcggtgttcc tgctcaacgt ggcgctcacg cggcagatcg
      241 gcacgcagcg gtcgcagatc gccgcactga aggcgctcgg ctgcgacgac cgtgcgatcg
      301 ggctgcacta cctgaagttc gtgctcgtga tcgtgctgat cggcgggccg atcggcgtcg
      361 cgggcgggta ctacttcggc tacgcggtcg cgcagcttta tgcgcgcttc ttccgcttcc
      421 ctgatttcga gttccgcatg ctggcgtgga ttccgttgtc tgccgtgctg ctgtcgatgt
      481 tcgctgcgat cgcaggcgtg gtcactgcgt tgcggcgcgt ggtgcggttg ccgccggcgg
      541 aagcgatgcg accggccgcg ccggcgacct tccggccgac gctgatggag gcgatcgggc
      601 tcggacacct gtattccccg gccgtgcgaa tggtcgtgcg cgacatcgag cgcaagccgc
      661 tgcgcgcaat tctgacgacg ttcggcatcg ccaccgcggt ggcggtgatg atcgccggca
      721 cgtggtggcg cgacgcggtc gactacctgc tcgacgtgga gatccgcatg cgcgaccggc
      781 aggaagtcag catcgcgttg accgaaccca cgtcggcgag cgcgatctac gaactggcgc
      841 gcctgcccgg cgtgctgcgc gccgagcccg atcgcgacgt cgcggtacgg ctgtccagcg
      901 gcccgcgtac gtatcgcacg tcattgagcg gcttgcgcgc cgacgggcag atgcggccgc
      961 tgctcgatgc gagactgcag cgcatcgccc tgccgagcgc cggcatcgtg ctgaaccggc
     1021 gcctcgcgga gcggctggat ctgaagctgg gcgacccggt gcgcgtcgag gtgctgcagg
     1081 gcacgcgcgc ggaggcgacg ctcacggtca gcggtttcag ccacgagctg atgcagctgc
     1141 cggcctacat cgactacgac gcgctgaacc ggctgctcgg cgaaggcgac gcgctgtcgg
     1201 gcgcgcgcct gctgatcgac cgcacgcagc gcgcggcgct ctttgcccag atcaagcaga
     1261 cgccgcgcgc ggcggtggcg gtggagatcg gcccgatcat ccgcaacgtg cgcgagaaca
     1321 ccgcgcgcaa catcctgttc ttcacgtccg tgatggcggt gatgggggcg gcgatcgcgt
     1381 tcggcgtcgt ctacaacaac gcgcgcatcg cgctggcgga gcgcgcctgg gacctggcca
     1441 gccttcgcgt actcgggttc acgcgcggcg aggtgtcggg gctgctgctc ggcgagctgg
     1501 cggtcgagct ggccgtggcg ctgccgctcg gcgccgtgct ggggcactgg ctgtcctggg
     1561 gcatcctgca gatgatgcag cacgagacga tgcagctgcc gttcgtgatc gaaccgggca
     1621 cgtacgcgtt cgcctgcctg gtcgtgctga tcgccggcgc cgcgagcgcg ctggtggtgc
     1681 ggcagcggat cgatcacctc gatctggtgg ccgtattgaa gacaagggaa tgaggcgcgg
     1741 atgaagcgca gacagtggat cctgatcgga gcgggttccg cattgct
//
LOCUS       DBNHBY010000074         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1018995, whole genome shotgun sequence.
ACCESSION   DBNHBY010000074 DBNHBY010000000
VERSION     DBNHBY010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1018995"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..>2113
                     /locus_tag="AC16HB_01760"
     CDS             340..>2113
                     /locus_tag="AC16HB_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5605543.1"
                     /translation="MASIAPSGRSAEAAEPLRLRFDAFDLDEADARLTRDGASIAMPP
                     KAFAVLCALARQPRLLMTKDALLDAVWGHQFVSESVLKTTISELRAALADDARQPRYI
                     ETVSRRGYRFIGTVSARTASTVEPIPALMQVPSLTGRQGALARLHAAWERAATGQRQV
                     FWIAGEPGVGKSTLIDHFIGALGPIMSARGQCVEQYGAGEPYLPVLEALAQLCRVDPA
                     LTPLLRSVAPTWLLQLPWLASESEREALRRELAGTSQDRMLREFGELLDRYTQVRPLL
                     LVTEDLHWSDHATVHLMNHLARRRTSARLMWLASFRLAEVIAEDHPLKALRHELRLHR
                     LCDELMLEPFSEQEVADYLAERMPAFRASEPFVRAVHARTDGLPLFVTHVIDDLLAQG
                     SLRAEDGVPAEARLASMAIPENLAGIIEQRIARLAPDQRAVLEAASVCGVEFRPRTVA
                     DALESDSMWVGAQCEELARQQQWLNSATVDRRADGTLDARHAFRHALYRHVFYERIGA
                     LARAQLHRRIGGALERDRGAGVAVAPAELATHFERGHEPMTALRHYAEAAESALRHFA
                     PSDAMSLAAQALDVLPHAPAGPMRD"
BASE COUNT          328 a          726 c          719 g          340 t
ORIGIN      
        1 gagcacgggg ttctgcatac cctcgttcct ggtttggctc ttcttcggac ggggtcgtct
       61 gcgtgcagta gggcgccgcc gcgaggaacg cctgattcga ctgcgtgcgt catcgcacgt
      121 gccggtaacg gacggtggtt gcccgaaaat gcagcgctcc aagcgcggat cgacgaagtg
      181 agccggccgc tgtccacggc atcgaacgag gccgttattc ccctttcaaa ggcatgggga
      241 ctggcattgc ggagcgagcg cggttgcagc ggtcgttgaa tcacttcatt cttgccgcat
      301 cgccagcgcg gaggcgcgcc aaaagcgcgc ggcttgggca tggcatccat tgcgccgtca
      361 ggtcgatctg cggaagcggc cgaaccgctg cggctgcgct ttgacgcgtt cgatctggat
      421 gaagccgatg cgcgcctcac gcgcgacggc gcgtcgatcg ccatgccgcc gaaagccttt
      481 gcggtgctgt gtgcgttggc gcggcaaccc cgactgctga tgaccaagga cgcgctgctc
      541 gatgcagtgt gggggcatca gttcgtcagc gagtcggtgc tgaagaccac gatcagcgaa
      601 ctgcgcgcgg cgctcgccga cgatgccagg cagccacgct acatcgagac cgtgtcgcgc
      661 cgcggctacc gcttcatcgg cacggtcagc gcgcgaacgg catcgacagt ggagccgatt
      721 cccgcgctca tgcaggtgcc ttcgctcacc ggaaggcagg gcgcccttgc ccggttgcat
      781 gccgcctggg agcgcgccgc cacgggtcag cgccaggtgt tctggatcgc cggtgaaccc
      841 ggtgtcggca aatccacgct catcgatcat ttcatcggcg cgctcggacc catcatgagc
      901 gcgcgcggcc agtgcgtcga gcagtacgga gccggcgaac cctatctgcc ggtgctggag
      961 gcactcgcgc agttgtgccg cgtcgacccg gcgctgacgc ctttgcttcg atcggtcgcg
     1021 ccgacctggc tgctccagtt gccgtggctc gccagcgaat ccgagcgcga ggcattgcgg
     1081 cgcgagctgg cgggcacgag tcaggaccgc atgctgcgcg aattcggcga actgctcgat
     1141 cgttacacgc aggtccggcc gctgctgctc gtgacggagg atctgcactg gagcgaccac
     1201 gccaccgtcc acctgatgaa ccacctggcc cgccgccgca cgtcggcgcg tctgatgtgg
     1261 ctcgcgagct tccgcctcgc cgaggtgatt gcggaagatc acccgttgaa ggcgctgcgc
     1321 cacgaattgc ggctgcaccg cctgtgcgac gagctgatgc tcgaaccatt ctccgagcag
     1381 gaggtcgccg attacctcgc cgagcgcatg cccgcgttcc gcgcgtcaga gccgttcgtg
     1441 cgcgcggtgc acgcgcgcac cgacggcctg ccactgttcg tcacgcacgt gatcgacgat
     1501 ctgctcgccc aaggctcgct gcgcgcggag gacggcgtgc ccgccgaagc gcggctggcg
     1561 tcgatggcaa tccccgagaa cctggccggc atcatcgagc agcggatcgc acgcctggcg
     1621 cccgaccagc gtgccgtcct cgaagccgcc agcgtgtgcg gcgtggagtt ccggccacgc
     1681 accgtggccg acgcgcttga aagcgacagc atgtgggtcg gtgcgcagtg cgaagaactt
     1741 gcacgccagc agcagtggtt gaacagcgcg accgtcgatc ggcgcgccga cggcacgctc
     1801 gacgcgcgcc atgccttccg tcatgcgctg taccggcacg tcttctacga acggatcggt
     1861 gcgctcgcgc gcgcgcaact gcaccggcgc atcggcggcg cgctcgaacg ggaccgcggc
     1921 gcgggggtcg ccgtcgcacc cgccgagctc gccacccact tcgaacgcgg ccacgagccg
     1981 atgacagcgc tgcgccacta tgcggaagcg gcagagagcg cattgcgtca ctttgcgccg
     2041 agcgacgcca tgagcctggc cgcgcaggcg ctcgacgtct tgccgcacgc accggccggc
     2101 cccatgcgcg acg
//
LOCUS       DBNHBY010000075         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_477750, whole genome shotgun sequence.
ACCESSION   DBNHBY010000075 DBNHBY010000000
VERSION     DBNHBY010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_477750"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="AC16HB_01765"
     CDS             complement(<1..1160)
                     /locus_tag="AC16HB_01765"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5605544.1"
                     /translation="MPGHPTLRRRHARLGLVAAALCGVAGLVAIAAGSRAQPLADANG
                     VLVPVTGPIGPATSDYFVRQLQMAEQTGARLVVMRIDTPGGLDTAMRDMIQAILASDV
                     PVIAYVAPSGARAASAGTYLMYASHVAAMAPATNLGAATPVQIGGGGEQPGRAEPKDA
                     KGDGKAGSPDGTHALPGSASERKAVNDAVAYLRGLAELRGRNIDWAERAVREGASLPA
                     DEALAAGVIDLIATDLPELLRAVNGRVVKLEKREVTLDTAGLVLRETQPDWRTELLQL
                     ITNPNVAYLLMLIGIYGLLLEGYNPGTVLPGVVGAISLLLALFAFQVLSVNYAGLALI
                     ALGVLLIVAESFVPSFGTLGLGGIVAFVFGSIMLLDRDVPGFGIAWQLIAVMA"
     gene            1405..2229
                     /locus_tag="AC16HB_01770"
     CDS             1405..2229
                     /locus_tag="AC16HB_01770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5605545.1"
                     /translation="MQQVRPSVYVIATPTSNVTVWTGNDGVVLVDSGAAAQAGELIAA
                     VTRLSPNPLRFVINTQRRADHMGGNEAAARKGAVVVGHDALRELRGRASASATAAGEF
                     PNTSAAARPMLTLTDSIALHLNGDRLDVLHVADAHSSSDLVARLATADVVSLGDIFWN
                     GQYPFIDLDDGGSLAGIVAAVEAALARSTARTVIVPGHGPVATRADLAAYRDMLVGVG
                     RQVREAIEAGQGVDEVLAAKPTAEYDARFARPGAAVTAEDFVRTVYRDLAGPRSPR"
     gene            complement(2272..>2945)
                     /locus_tag="AC16HB_01775"
     CDS             complement(2272..>2945)
                     /locus_tag="AC16HB_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5605546.1"
                     /translation="VFGAVGTAGQRCTTTRRVFVHESRQAELERRLVAAYAQVRIGDP
                     LAHGTLMGPLIDAAAVERFEGAVRQAVDQGGRLLCGGKRLDRPGHFVQPAIVAARGDW
                     EIVRTETFGPVLYLIPYASLDEAIAAQNEVAHGLSSAIFTDRLQHAERFLSAAGSDCG
                     IANVNIGTSGAEIGGAFGGEKDTGGGRESGSDSWKAYMRRQTCTVNWGNDLPLAQGIN
                     FDVSG"
BASE COUNT          448 a         1019 c         1055 g          423 t
ORIGIN      
        1 gccatgacgg cgatcagctg ccaggcgatg ccgaagccag gcacgtcgcg gtcgaggagc
       61 atgatcgatc cgaacacgaa ggcgacgatg ccgccgagcc cgagggtgcc gaacgacggc
      121 acgaacgact ccgccacgat gagcaggacg ccgagcgcga tcaacgccag gcccgcgtag
      181 ttcaccgaga gcacctggaa cgcgaacaac gcgagcagca ggctgatggc gccgacgacg
      241 ccgggcagga ccgtgccggg gttgtagcct tcgagcagca ggccgtagat gccgatcagc
      301 atgagcagat aggcgacatt cgggttcgtg atcagctgca gcagttcggt gcgccagtcg
      361 ggctgggttt cgcgcagcac cagccccgcg gtgtccagcg tcacctcccg cttctccagc
      421 ttcacgacgc gcccgttcac ggcgcgcagc agctcgggca ggtccgttgc gatgaggtcg
      481 atcacgccgg cggccagcgc ctcgtccgcc ggcaggctgg caccctcgcg cacggcgcgt
      541 tcggcccagt cgatgttgcg gccgcgcagt tcggccagcc cacgcaggta ggccacggcg
      601 tcgttcacgg ccttgcgctc cgaggcggag ccgggcaggg cgtgcgtgcc gtcgggcgaa
      661 ccggccttgc cgtcgccttt ggcgtccttg ggttcggccc ggcccggctg ctcgccgccg
      721 ccgccgatct gcacgggtgt cgccgccccc aggtttgtcg ccggtgccat ggccgcaacg
      781 tggctggcgt acatgaggta cgtgcctgcg ctggcggcgc gggcgccgct gggtgcgacg
      841 taggcgatca ccgggacgtc gctggcgagg atcgcctgga tcatgtcgcg catggcggtg
      901 tcgagaccgc ccggggtgtc gatccgcatc acgaccagcc gggcgccagt ctgctcggcc
      961 atctgcagtt gccggacgaa gtaatcgctg gtcgcggggc cgatggggcc ggtgacgggc
     1021 acgagcacgc cattcgcatc ggccagtggc tgggcgcgcg agccggccgc gatggcgacc
     1081 agccctgcca cgccgcacag ggctgctgca accagtccga gccgcgcgtg gcggcgccga
     1141 agcgtcgggt gaccaggcat ggaccggatc ataccgcggt gaccgctgcg cgcgttgccc
     1201 tatactcggc gcagaaggag aacgacgatg agtgcggttc gaaggtcgcc gtggtgccta
     1261 atcctgggat cggtggcact gctggcgggg gcgggaatcc ttgcacccgc gggcgcggcg
     1321 ccagccccgt ccagcccgcc tccaacaggc ggtgagagcc cagccaggtc ggctgaaacg
     1381 gcgaacgcca gccccgacct gcgcgtgcaa caggtgcgtc cgtccgtcta tgtgattgcc
     1441 acgcccacga gcaatgtgac ggtgtggacc ggcaacgacg gcgtcgtgct cgtcgactcc
     1501 ggcgccgcgg ctcaggccgg cgaactgatc gccgccgtga cgcgactgtc gccgaatccc
     1561 ctgcgtttcg tcatcaacac gcagcgtcgc gcggaccaca tgggtggcaa cgaagcggcc
     1621 gcacgcaaag gggccgtggt cgtcggacac gacgcgctgc gcgagctccg gggtcgagcc
     1681 tcggccagcg cgacagcggc gggcgagttt ccaaacacat ccgccgcggc gcgaccgatg
     1741 ctgacgctca ccgattcgat tgcgctgcac ctgaacggcg atcgtctcga cgtgctgcac
     1801 gtggccgacg cgcattcctc gtcggacctg gtcgcgcgcc tggccaccgc ggacgtggtg
     1861 agcctcggcg acatattctg gaacggccag tacccgttca tcgacctcga cgacggcggc
     1921 tcgctggccg gcatcgtggc ggcggtcgaa gccgcactcg cgcgttcaac ggcgcgcacg
     1981 gtgatcgtgc cagggcacgg ccctgtggcc acgcgagccg acctcgcggc ctaccgcgac
     2041 atgctggtcg gcgtcggtcg ccaggtgcgg gaagccatcg aggccgggca gggcgtcgac
     2101 gaagtgctcg cggcgaagcc gacggcggag tacgacgcca ggttcgcgcg gccgggcgcc
     2161 gccgtgacag ccgaggactt cgtccgcacg gtctatcgcg acttggcggg accgcgctcg
     2221 ccgcgctgac gcgccgcgag cgcgagatcg caacggaagt cgtcgcgacc ctcagccgct
     2281 cacgtcgaag ttgatgccct gcgcgagggg caggtcgttg ccccagttca cggtgcaggt
     2341 ctggcggcgc atgtaggctt tccacgagtc ggagcctgat tcgcgtcctc cgcccgtgtc
     2401 tttctcccca ccgaaggccc caccgatctc ggcgccggac gtgccgatgt tgacgttggc
     2461 gatcccgcag tcggaaccgg ccgcggacag gaagcgctcg gcgtgctgca ggcgatccgt
     2521 gaagatcgcc gacgacaggc cgtgcgccac ctcgttctgc gccgcgatgg cctcgtcgag
     2581 cgaggcgtac ggaatgagat acaggacggg accgaacgtc tccgtgcgca cgatctccca
     2641 gtcaccgcgt gccgccacga tcgctggttg gacgaagtga ccggggcgat cgagtcgctt
     2701 gccaccacac agcaggcgtc cgccttggtc gacagcctgg cgcacggcgc cttcgaagcg
     2761 ctcgaccgct gccgcgtcga tcaacggccc catcagggtg ccgtgcgcga gcggatcgcc
     2821 gatgcgcacc tgggcgtagg cggcgacgag gcggcgttcc agttccgcct gtcgcgactc
     2881 gtgcacgaac acgcgtcgcg tcgtggtgca gcgctggccg gcggtgccga cggcgccgaa
     2941 cacga
//
LOCUS       DBNHBY010000076         2388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1222138, whole genome shotgun sequence.
ACCESSION   DBNHBY010000076 DBNHBY010000000
VERSION     DBNHBY010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1222138"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..699
                     /locus_tag="AC16HB_01780"
     CDS             280..699
                     /locus_tag="AC16HB_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5605547.1"
                     /translation="MRTLIAFDGSEHALRAVDFVCRYPHILGDRAQVTVVFVTTTSPL
                     RAVAALGAEGSAPLPAEAERTVRPALEKLRQSGIDATLADLTGDAGLEICQLARDDGY
                     DLVVIGSHGRGLLKRAVLGSVAERVLSHCKVPVLIVR"
     gene            complement(686..1150)
                     /locus_tag="AC16HB_01785"
     CDS             complement(686..1150)
                     /locus_tag="AC16HB_01785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5605548.1"
                     /translation="MIANYEVGLARAAEAGRIALLSRAVIEHGLAWSWTPQRVLRSVR
                     DPATNAIVARERGSLLGFAIMRYGDANAHLLLLAVDGTRRRRGVGSALLEWLDVTVRT
                     AGLESIRVEVRAANDPARAFYRRHGFDQVRVLRGYYQGVDDAIVLAKRISAR"
     gene            complement(1147..1533)
                     /locus_tag="AC16HB_01790"
     CDS             complement(1147..1533)
                     /locus_tag="AC16HB_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR5605549.1"
                     /translation="MTIKVVRLGTPRAPDEGPRLGTVRRPPRGVPKNEFARRDFYDAW
                     LPNLAPSAELVKFAQGARSERDWSVFKRRYRTEMAAPDNARVLDALAALSRHANFAVG
                     CYCEDETHCHRSVLRELLREHGASVE"
     gene            complement(1577..>2388)
                     /locus_tag="AC16HB_01795"
     CDS             complement(1577..>2388)
                     /locus_tag="AC16HB_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PI-PLC domain-containing protein"
                     /protein_id="HIR5605550.1"
                     /translation="IPANPAPRCCTSDLTLAEFKSLKGKMDASVPSATTPQGFLGGTA
                     TWRTDLYNSRGTLMKHSEYIQIVKALGGKFTPELKYPNNPDRVVAIFGSQANYAQKMI
                     DEYKAAGIPPSQVWPQSFFLGDILYWIQNEPQFGHQAVFLDSSAYPARLSVAQLNELK
                     AQGLRIFAPPMQLLLALNASNQIVPSQYALDIKAAGLDIITWTFERVDLRTGAAGKGS
                     YYAFDPTGAAVTKDSDMYKALDVLAKGVGILGIFSDWPATVTYYANCMGLK"
BASE COUNT          361 a          846 c          799 g          382 t
ORIGIN      
        1 ccctccccgc aacggctctt cgtcgtcgcc gaattcacct ttcgggatcg accacgcgcc
       61 ggtgtccttg cgcgcccaga acgggccgcc cggatgcacg agaaacacct cggcccgccc
      121 cgcgcgctcg cggaacagaa gcagaccggc gctcttcttc ggcatcgcga tcgagcgtaa
      181 cgcgctttcg cgcagccgcc cccgtgaccg gcatcaaccg cacccgacgc gcttctgcaa
      241 ccatcgcctt gacgcacgga acgacgagga gcaacgctca tgcgcaccct gatcgccttc
      301 gacggcagcg agcacgcttt gcgcgcggtc gatttcgtct gtcgttaccc gcacatcctc
      361 ggcgaccgcg cgcaggtcac ggtggtcttc gtcacgacga catcgcccct gcgcgccgtc
      421 gcagcgctcg gcgccgaggg atccgcgccg ctgcccgccg aggcggagcg caccgtacgg
      481 cccgcactgg agaagctgcg gcaatccggc atcgacgcga cgctggcgga cctcacgggc
      541 gacgccggac tggagatctg ccagctcgcg cgcgacgacg gctacgacct ggtcgtgatc
      601 ggctcgcacg ggcgcgggct cctgaagcgc gccgtgctcg gctcggtcgc cgaacgggtg
      661 ctgagccact gcaaggtgcc ggtgctcatc gtgcgctgat gcgcttggcc agcacgatcg
      721 cgtcgtccac gccctggtag tagccgcgca gcacgcgcac ctgatcgaag ccgtgccggc
      781 ggtagaacgc gcgcgccgga tcattggcgg cgcgcacctc gacgcggatc gattcgaggc
      841 ccgcggtgcg cacggtcacg tcgagccatt cgagcagcgc cgaaccgacg ccacggcggc
      901 gccgcgtccc gtcgaccgcg agcagcagca ggtgcgcgtt ggcgtcgccg tacctcatga
      961 ttgcgaagcc gagcaggctg ccgcgctcgc gcgcgacgat cgcgttggtg gccggatcgc
     1021 gcacgctcct gagcacgcgc tgcggcgtcc acgaccacgc caggccgtgc tcgatcacgg
     1081 cgcgcgacag cagcgcgatc cgcccggcct cggccgcgcg cgccaacccg acctcgtagt
     1141 tggcgatcac tcgacgctcg cgccgtgttc gcgcagcagt tcgcgcagca ccgagcgatg
     1201 gcagtgcgtc tcgtcctcgc agtaacatcc gacggcgaaa ttcgcgtggc gcgacagcgc
     1261 cgcgagcgcg tcgaggacgc gcgcgttgtc cggcgccgcc atctcggtgc ggtagcggcg
     1321 tttgaaaacg gaccagtcgc gctcgctgcg cgcgccctgt gcgaacttga ccagctccgc
     1381 gctcggcgcg aggttcggca gccacgcgtc gtagaagtcc cgccgggcga attcgttctt
     1441 cggcacgccg cgcggcggac gccgcacggt ccccagccgc ggaccttcgt ccggcgcgcg
     1501 cggggtcccg aggcggacta ccttgatcgt catggcggcc tcctgcgact gaggccgcct
     1561 attctgcgcc gaaccgctac ttcaatccca tgcagttggc gtagtaggtc accgtcgccg
     1621 gccagtcgga gaagatgccg aggatgccca cgcccttggc gagcacgtcg agcgccttgt
     1681 acatgtcgct gtccttcgtc accgcggcgc ccgtcgggtc aaacgcgtaa tagctgccct
     1741 tgcccgccgc accggtgcgc aggtcgacgc gctcgaaggt ccaggtgatg atgtcgagcc
     1801 cggccgcctt gatgtcgagc gcgtactgcg acggcacgat ctggttgctg gcattgagcg
     1861 cgagcagcag ctgcatcggc ggcgcgaaga tccgcaagcc ctgggccttg agctcgttga
     1921 gctgggcaac cgacagccgc gccggatacg cgctggaatc gaggaagacc gcctgatgcc
     1981 cgaactgcgg ctcgttctgg atccagtaca ggatgtcgcc gaggaagaac gactgcggcc
     2041 agacctggct cggcggaatg ccggccgcct tgtactcgtc gatcatcttc tgcgcatagt
     2101 tggcctggct gccgaagatc gcgaccacgc ggtccgggtt gttcggatac ttgagttccg
     2161 gcgtgaactt gccgccgagc gccttgacga tctggatgta ctcgctgtgc ttcatcaagg
     2221 tcccgcggct gttgtacaga tcggtgcgcc aggtggccgt gccgccgagg aaaccctgcg
     2281 gcgtcgttgc gctgggaacg gacgcatcca tcttgccctt cagcgacttg aactccgcga
     2341 gggtcaggtc actggtgcag catctcgggg ccggattggc ggggatcg
//
LOCUS       DBNHBY010000077         7237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1222233, whole genome shotgun sequence.
ACCESSION   DBNHBY010000077 DBNHBY010000000
VERSION     DBNHBY010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1222233"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16HB_01800"
     CDS             complement(<1..489)
                     /locus_tag="AC16HB_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase light chain"
                     /protein_id="HIR5605551.1"
                     /translation="MLIQWFDRIVEGRVRKVAQDTSRRSLLVGVGRTLVGAAFVLPVL
                     PFDRSTRAAEGRAKPQKTDVDCDYWRYCALDGFLCTCCGGSVSQCPPGTEPSKVTWIG
                     TCRSALDGKDYLISYNDCCGVTACGRCLCNGNEGERPGYRMGVHNDINWCMANSSTMY
                     HCT"
     gene            complement(513..1103)
                     /gene="mauD"
                     /locus_tag="AC16HB_01805"
     CDS             complement(513..1103)
                     /gene="mauD"
                     /locus_tag="AC16HB_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine dehydrogenase accessory protein
                     MauD"
                     /protein_id="HIR5605552.1"
                     /translation="MTEALVISNVLLWLVVLALLVAVIALARQVGILYERVAPMGALT
                     LDAGPAVGTAAPAMSLRALDGRAVELGTPARASTLLFFLSPTCPVCKKLLPILRSIRA
                     AEASWLDVVLASDGELAEHQAFIARQGLGDFAYVLSRDLGMAFRVGKLQYAVLMDEGG
                     LVRAKGLVNSREQIESLFNARDLGVGSVQELVRQKG"
     gene            complement(1105..1677)
                     /locus_tag="AC16HB_01810"
     CDS             complement(1105..1677)
                     /locus_tag="AC16HB_01810"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR5605553.1"
                     /translation="MLSDAIVVDAVIARACGAAVALILMIGAIDKLRDRELFEAIVEN
                     YRVLPPGPASRWFAALLPVAEIATAGMLLWPSMRGTGALAAVALLSMFSLAIALSLLR
                     GRRDVDCGCGGASGRQTLAWWLIVRNAALALLALIGAGDGAVRDLAWLDAFTAVAGTL
                     ALLALYVFFNQLGANAPRLNSLRHPRREEV"
     gene            complement(1680..2903)
                     /locus_tag="AC16HB_01815"
     CDS             complement(1680..2903)
                     /locus_tag="AC16HB_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - aliphatic amine dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HIR5605554.1"
                     /translation="MSKFNTGAPLCARPALSQCAAIRRTRVLGIAVAAVLSFASAPVC
                     AQSKDGVETVSVATLAPATPDRLYVADVAITHIVDGKLHILNGRTMKYEGTVATALAG
                     NFTLSADRKELYVATTYFTRLNRGERVDQVDIYDAATLKLTGEVSIPAKHAQALPYRG
                     YVRASSDGRWLFVQNATPATSVSIVDLKAKKFVVEVPTPGCWALLTVPSEPARFASIC
                     GDGSLLTVALDAEGKVASQRRMPKFFDPDVDPIFIHAEYDGDRAYFVSFKGMVHSVNL
                     AGEQPGFGAPWPLVQGADSKKNWRPGGYQPLALDAKNGRLYVGMHPDGKEGSHKEPAR
                     EIWAFDTAKKARVARVPGSMSIALAVTRGDKPLLFGIDGAKNALVSWDVQGTPRKRKE
                     QLAPMGDSATLIEVQ"
     gene            complement(3200..4885)
                     /locus_tag="AC16HB_01820"
     CDS             complement(3200..4885)
                     /locus_tag="AC16HB_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5605555.1"
                     /translation="MSKTLPRIPAGRLSLVRGATDVPLSEQTVHDVLAAAVRRWPQQD
                     AAVFREQGIRWTWSQLGLEIERAAAGLWALGVRKGDRVGIWSPNRSEWLVTQFATARI
                     GAILVNVNPAYRLAELEYALNKSGVSVLVTAAAFKSSDYIGMLQTLGFGSATGAKLPD
                     LKTVVRLGDDATAGMLNWSRMLEAGAAHAAALPAADAQNCREPINIQFTSGTTGAPKG
                     ATLTHHNIVNNALAVAQCMRLTERDSLCIPVPLYHCFGMVLSVLACVVSGAKMVFPGE
                     GFDPRATLAAVQAEKCTALHGVPTMFIAELDHPEFRQFDLSSLRTGIMAGAPCPIEVM
                     RRVQSDMHMHEVTIAYGMTETSPVSFQSATDDPIDKRVTTVGRVLPHLEVKIIDAEGR
                     IVPVGKKGELCTRGYSVMLGYWDDEKRTREAVRDGWMHTGDLATIDAEGYCNIVGRVK
                     DMVIRGGENVYPREVEEFLFRHPKVATVQVFGVPDDRYGEELCAWIVTKPGVDCTEAE
                     IRDFCRDQIAHYKVPRYIRFVPELPVTVTGKPQKFLMREAMMRELGLHVAATA"
     gene            5119..6120
                     /locus_tag="AC16HB_01825"
     CDS             5119..6120
                     /locus_tag="AC16HB_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5605556.1"
                     /translation="MTVASDATRTAAACHPPPEFAFARNESHDADEAAQRLRAWDQVY
                     RQLAPGRFAGLTTDLWFESMQVFRERSNRRIYQAGRAWPGSVTFGIPLAMEGTARFCG
                     QPMDLDSMLVLGSCGELDFLTSPTLDIVGISMRQEKLASLGAARAAFESECGRAPNRL
                     VQMNGPVAARVRALVQSLFEAIEADPDRLSSAGVREQIRDDVMEGLAAAAHDSVAPPM
                     HAERHANQRAIVQRATDYVLSHTDEPIFVERLCEVIGVSRRSLQYAFEHVLGTSPVEY
                     LRAARLHGVRRELKSTSRSVASIQDIAARWGFWHLGHFTTHYRQLFGCRPSETPRPA"
     gene            complement(6192..6995)
                     /locus_tag="AC16HB_01830"
     CDS             complement(6192..6995)
                     /locus_tag="AC16HB_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5605557.1"
                     /translation="MSPPQPAALIAEDEPLLARTLERLLRDAWPELRLVGSVADGLTA
                     TSLALEQTPDVLFLDIQMPGRSGLDVAEAVADEWPDDRPQPLFVFVTAYDEYAVAAFE
                     RAAVDYLLKPVTPERLVQTVRRLQQRLAERAAATHPGETAELVRSVQAIGAASDLPTE
                     RIRIIRAAAGNKVRMIALTDVILLEAADKYVNVVTEQGDALVRMSLRELAQRLEGVEF
                     LQVHRSVLVNATRIRSATRDELGHYWLELAGLARPVKVSRAFSHLFRPM"
     gene            complement(6992..>7237)
                     /locus_tag="AC16HB_01835"
     CDS             complement(6992..>7237)
                     /locus_tag="AC16HB_01835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5605558.1"
                     /translation="GIEPKVEGGTVTIAAERLAAGEVEIAVADTGIGTGEAAEEGAGY
                     GLAHVRERLRSVFGARAMLTLTPNLPRGTRATVRFPA"
BASE COUNT         1173 a         2587 c         2322 g         1155 t
ORIGIN      
        1 cgtgcagtgg tacatcgtgc tcgagttggc catgcaccag ttgatgtcgt tgtgcacgcc
       61 catccggtag cccggccgtt cgccttcatt gccgttgcac aggcagcggc cgcacgcggt
      121 cacgccgcag cagtcgttgt agctgatcag atagtccttg ccgtccagcg cgctgcgaca
      181 ggtaccgatc caggtcacct tggaaggttc ggttcccggc gggcactgcg aaacggaacc
      241 gccgcagcag gtgcacagga acccatcgag cgcgcaatag cgccagtaat cgcaatcgac
      301 gtccgtcttc tgcggcttgg cccgtccctc cgccgcgcgc gtcgatcgat cgaacggcag
      361 cacgggcaga acgaacgcgg ctcccaccag cgtgcgcccg acgccgacca gcaggctgcg
      421 ccgcgaggtg tcctgcgcca ccttgcgcac gcggccctcg acgatgcgat cgaaccattg
      481 aatcagcatg acttcctccc ggcgacgccg cctcagcctt tttgcctgac cagttcctgc
      541 accgagccca cgcccaggtc gcgcgcattg aacaggcttt cgatctgctc gcgcgagttg
      601 accaggccct tggcgcgcac cagaccgccc tcgtccatca gcaccgcata ctgcagcttg
      661 ccgacgcgaa atgccatgcc cagatcacgg ctgagcacgt aagcgaagtc gcccagaccc
      721 tggcgggcga tgaaggcctg gtgttcggcg agttcgccgt cgctggcgag cacgacatcg
      781 agccagcttg cttcggctgc gcgaatgctg cgcaggatcg gcagcagctt cttgcaaaca
      841 gggcaggtcg gcgacaggaa gaagagcagc gtgctcgcgc gcgccggcgt gccgagttcg
      901 accgcgcggc catcgagcgc gcgcaggctc atcgcaggcg ccgccgtccc caccgccggt
      961 cccgcgtcga gcgtcagcgc gcccatcggc gccacgcgtt cgtacaggat gcccacctgc
     1021 cgggcgagcg cgatgaccgc gaccagcagc gccagcacca ccagccacag cagcacgttc
     1081 gagatcacca gcgcttccgt catttcagac ttcctcgcgc cgcgggtggc gcagcgagtt
     1141 caggcgcggc gcattggcgc cgagctgatt gaagaatacg tacagcgcca gcagcgccag
     1201 ggtgccggcc accgcagtga acgcatcgag ccatgccagg tcgcgcactg cgccgtcgcc
     1261 ggcgccgatc agcgcgagca gcgccagtgc cgcattgcgc acgatcaacc accacgccag
     1321 cgtctgccgc ccgctcgcgc cgccgcagcc gcagtcgacg tcgcgccggc cgcgcagcag
     1381 gctcaatgcg atcgcgagcg aaaacatcga tagcagtgcg acggcggcaa gcgcgccggt
     1441 tccgcgcatg gacggccaca gcagcatgcc ggccgtcgcg atctcggcca ccggcaacag
     1501 cgccgcaaac cagcgggacg ccggcccggg cggcagcacg cggtagttct cgacgatcgc
     1561 ctcgaacagc tcgcggtcgc gcagcttgtc gatcgcgccg atcatcagga tcagtgcgac
     1621 cgccgcccca caggcgcgcg cgatcacggc atcgacgacg atcgcatcgg acaacatgcc
     1681 tactgcacct cgatcagcgt ggccgagtca cccatcggcg ccagctgttc cttgcgcttg
     1741 cgcggcgtgc cctgcacgtc ccacgacacc agcgcattct ttgccccgtc gatgccgaac
     1801 aggagcggct tgtcgccccg cgtcaccgcc agcgcgatcg acatcgagcc cggcacgcgc
     1861 gcgacgcgcg ctttctttgc cgtatcgaac gcccagattt cgcgcgccgg ctccttgtgg
     1921 ctgccctcct tgccgtccgg atgcatgccg acgtacagcc ggccgttctt cgcatccagc
     1981 gccagcggct gatagccgcc cgggcgccag ttcttcttgc tgtccgcgcc ctggaccagc
     2041 ggccacggcg caccgaatcc cggctgctcg ccggccagat tcaccgaatg gaccatgccc
     2101 ttgaaggaaa cgaagtacgc ccggtcgccg tcatattccg cgtgaatgaa gatcgggtcg
     2161 acgtcgggat cgaagaactt cggcatgcgc cgctgcgatg ccaccttgcc ttcggcatcg
     2221 agcgccacgg tgagcagcga cccgtcgccg cagatcgatg cgaagcgcgc cggctcggac
     2281 ggaacggtca gcagcgccca gcaccccggc gtcggcactt cgacgacgaa cttcttcgcc
     2341 ttcaggtcga cgatgctgac cgaggtcgcc ggcgtcgcgt tctgcacgaa caaccagcgg
     2401 ccatcggacg acgcccgcac gtagccgcgg taaggcagcg cctgcgcgtg cttggcgggg
     2461 atcgacactt cgccggtcag cttcagcgtc gccgcgtcgt agatgtcgac ctggtcgacc
     2521 cgctcgccgc gattcagccg cgtgaaatac gtggtcgcga catacagctc cttgcgatcc
     2581 gcggacagcg tgaagttgcc cgccaaggcg gtggcgaccg tgccttcgta cttcatcgtc
     2641 cggccgttga ggatgtgcag cttgccgtcg acgatgtgcg tgatcgccac gtcggcaacg
     2701 taaagccgat ccggtgtggc gggcgccagg gtggcgacgc tgaccgtctc cacgccgtcc
     2761 ttcgactgcg cgcagaccgg cgccgatgca aacgacagga ccgccgccac ggcgatgccg
     2821 agcacacgag tacggcgaat cgcggcgcat tgcgacagtg ccggcctggc gcagagaggc
     2881 gcgccggtat tgaacttcga catgatgtct cctgatcctg atcccgcgcg cgacctgcac
     2941 cgcatcatgc gcgcaccccg attgcggttc cgcgaagagc ccgcgtttcc tcgtccacgg
     3001 ccggaaacgc gcgacaaggt aacttgcttt ctgcgtccgc tgttatcccg gatcggcaat
     3061 ccgtggccgg atcttcgaag cgaaccgttg tgtctccctt tgccgagatc aagaaaaggc
     3121 gcacgcgtcc cggttggtgc gccgcaccct cccgcaaacc cgctgcggcc aatacgtagc
     3181 tcgcgccggc ccgcacccgc tacgcggtgg ccgcgacgtg caatcccagc tcgcgcatca
     3241 tcgcctcgcg catcaggaac ttctgcggct tgcccgtcac ggtgaccggc agttccggca
     3301 cgaatcgtat gtagcgcggc accttgtagt gggcgatctg gtcgcggcag aagtcgcgga
     3361 tctccgcttc ggtacagtcg acgcccggct tggtcacgat ccacgcgcaa agctcctcgc
     3421 cgtagcgatc gtccggaacg ccgaagacct gcaccgtcgc caccttcggg tggcgaaaca
     3481 ggaactcctc gacctcgcgc ggatagacgt tctcgccgcc gcgaatcacc atgtccttga
     3541 cccggccgac gatgttgcag tagccctccg cgtcgatcgt cgcgaggtcg ccggtgtgca
     3601 tccagccgtc gcgcaccgcc tcgcgcgtgc gcttctcgtc gtcccagtag cccagcatca
     3661 ccgagtagcc gcgcgtgcac agttcgccct tcttgcccac cgggacgatg cggccctcag
     3721 cgtcgatgat cttcacttcg agatgcggca gcacgcggcc gaccgtggtc acgcgcttgt
     3781 cgatcggatc gtcggtagca ctctggaacg acaccgggct ggtctccgtc atgccgtagg
     3841 cgatcgtcac ctcgtgcatg tgcatgtcgc tctgcacccg ccgcatcacc tcgatcgggc
     3901 acggggcccc ggccatgatg ccggtgcgca gcgaactcag atcgaactgc cggaactccg
     3961 gatggtcgag ttcggcgatg aacatcgtcg gcacgccgtg caaggcagtg catttctctg
     4021 cctgcaccgc ggccagcgtg gcgcgcggat cgaagccctc gcccgggaac accatcttgg
     4081 cgccgctgac cacgcacgcg agcaccgaca gcaccatgcc aaagcagtgg tacagcggca
     4141 ccgggatgca caggctgtcg cgctcggtca agcgcatgca ctgcgcgacc gccagcgcgt
     4201 tgttgacgat gttgtgatgc gtaagcgtgg cacccttcgg tgcgccggtc gtgccgctcg
     4261 tgaactggat gttgatcggc tcgcggcagt tctgcgcatc ggccgcgggc aacgcggcgg
     4321 cgtgtgccgc cccggcttcg agcatccggc tccagttcag catccccgcc gttgcatcgt
     4381 ccccgagccg cacgacggtc ttcagatccg gcaatttcgc gccggtggcg ctgccgaagc
     4441 cgagcgtctg cagcatgccg atgtagtcgc tggacttgaa cgccgccgcc gtgacgagca
     4501 cgctgacgcc cgacttgttc agcgcgtact cgagctcggc gagtcgatag gccggattga
     4561 cgttgacgag gatggctccg atgcgcgcag tggcgaactg cgtcaccagc cattcggagc
     4621 ggttcggcga ccagatgccg acccgatctc ctttgcgcac gccgagcgcc catagccccg
     4681 cggcagcgcg ctcgatctcc agccccagct gcgaccaggt ccagcggatc ccctgttcgc
     4741 ggaatacggc tgcgtcctgc tgcggccagc gccggactgc tgcggccagc acgtcgtgca
     4801 cggtctgctc ggacaacggg acgtcggtcg cgccgcgaac cagcgagagg cggccggccg
     4861 gaattctcgg aagtgtcttg ctcacgaatc cgctccctac tgatgcgccg caagcgcgag
     4921 aggcatgagc ctagcggcga tccggcggtc gcgttatccc ggatcggcaa cggttcggcc
     4981 ggcctcccga gcatccgcga cgcaacacct tgacgtcact gcattgccgg ccgcgcatcg
     5041 gatgccgcac aatggacgcc aaaccaaagc acgccgtgcg gattgccgca tgcaaagtgc
     5101 gcacagggag acatcaccat gacagttgcc agtgatgcga cgcgcaccgc ggcggcgtgc
     5161 cacccgcccc ccgagtttgc gttcgcgcgc aacgagagcc acgacgccga cgaggcggcc
     5221 cagcgcctgc gcgcctggga tcaggtctac cggcagctcg cacccgggcg gttcgcgggc
     5281 ctgacgaccg acctgtggtt cgagtcgatg caggtcttcc gcgagcgcag caatcgccgc
     5341 atctaccagg ccgggcgcgc gtggccgggc tcggtcacct tcggcattcc gctggcgatg
     5401 gaaggcacgg cccgcttctg cggccagccg atggatctcg actcgatgct ggtgctcggc
     5461 tcgtgcggag aactggattt cctgacctcg ccgacgctgg acatcgtcgg catctcgatg
     5521 cggcaggaga agctcgcatc gctcggcgcg gcgcgcgcgg cgttcgaatc cgaatgcggc
     5581 cgggcgccga atcgcctcgt gcagatgaat gggccggtcg ccgcccgcgt gcgggcactc
     5641 gtgcagtcgt tgttcgaagc gatcgaggcg gacccggatc ggctgtcctc ggccggcgtg
     5701 cgcgaacaga tccgcgacga cgtgatggaa ggcctcgccg cggcggcgca cgactcggtc
     5761 gcgccgccca tgcacgcgga gcgccacgcc aaccagcgtg cgatcgtgca gcgcgcgacc
     5821 gactacgtgc tgtcgcacac cgacgagccg atcttcgtcg agcgcctgtg cgaagtcatc
     5881 ggcgtatcgc gccgcagcct gcagtacgcg ttcgagcatg tgctcggcac gtcgcccgtc
     5941 gagtacctgc gcgccgcgcg gctgcacggc gtgcgccgcg aactgaaatc gacgtcacgc
     6001 agcgtcgcgt cgatccagga catcgccgcg cgctggggct tctggcatct aggccacttc
     6061 accacgcatt accggcagct gttcgggtgc cgtccgtccg agacgccgcg gccggcctag
     6121 ccgggcaacc aacgccgctc ctcacgctcg ccttccgggt cgccccgggg cgggacgtca
     6181 ttgtcacgag ttcacatcgg ccggaacagg tgggagaagg cgcggctcac tttcaccggc
     6241 cgtgcgagcc cggcgagttc gagccagtag tgcccgagct cgtcgcgagt cgcgctccga
     6301 atccgggtgg cgttgacgag caccgatcga tgcacctgca ggaactccac gccctcgaga
     6361 cgctgtgcca gttcgcgcag gctcatgcgt accagcgcat cgccttgctc ggtgaccacg
     6421 ttgacgtact tgtcggcagc ctcgagcagg atgacgtccg tgagcgcgat catgcgcacc
     6481 ttgtttccgg ctgcggcccg aatgatccgg atgcgctcgg tgggcaggtc gctcgcagcg
     6541 ccgatcgcct gcaccgaacg gaccagctcg gccgtttcgc ccgggtgcgt cgcggccgcg
     6601 cgttctgcca ggcgctgctg cagacgccgg accgtctgca ccaggcgctc cggcgtcacc
     6661 ggcttgagca gatagtcgac cgccgctcgc tcgaaagctg caaccgcata ctcgtcatag
     6721 gcggtgacga acacgaacaa cggctgcggt cgatcgtccg gccactcatc ggcgacggct
     6781 tcggcgacgt cgagcccgga tcgaccgggc atctggatgt cgaggaacag cacatccggc
     6841 gtttgctcca gcgcaagcga ggtcgctgtc aatccgtctg cgacgctgcc caccagccgc
     6901 agttcgggcc acgcgtcgcg gagcaggcgc tccagcgtgc gtgcgagcag cggctcgtct
     6961 tcggcgatca atgcggcggg ctgcggaggg ctcatgcggg aaacctgacg gtggcgcgag
     7021 tgccccgggg aagattcggg gtcagcgtca gcatcgcgcg tgcgccgaac acgctgcgca
     7081 gccgttcgcg aacatgtgcc aggccgtaac cggcaccctc ttccgcggcc tcccccgtgc
     7141 cgatcccggt gtcggcgacg gcaatctcga cctcgcccgc ggcaaggcgc tcggcagcga
     7201 tcgtgaccgt gcccccctcc accttcggtt cgatccc
//
LOCUS       DBNHBY010000078         4005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_613226, whole genome shotgun sequence.
ACCESSION   DBNHBY010000078 DBNHBY010000000
VERSION     DBNHBY010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_613226"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16HB_01840"
     CDS             <1..482
                     /locus_tag="AC16HB_01840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR5605559.1"
                     /translation="ARQHKQVALLFLDLNGFKRVNDGFGHAAGDRILQQVAARLVACI
                     RTSDTACRFGGDEFVILLPELEGQDGAIAVAEKLRAHLATPYVLDGTEIRLTASIGMA
                     IYPVDGHECADLVRVSDRAMYRSKTRVADVPGDHEPALQVRRRPAGFEESEVHRHAS"
     gene            complement(1670..2023)
                     /locus_tag="AC16HB_01845"
     CDS             complement(1670..2023)
                     /locus_tag="AC16HB_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605560.1"
                     /translation="MLANVAQLVFDDVEIVEQPFSGRRDGEALSCRSADQRMGFVQYA
                     AVLPHARNQRALARVILDDRLRNRQALRVLLEPFGAEQLRADRLLEFGEVGRTTGAGQ
                     AAEELCAAAHLSPAH"
     gene            2003..>4005
                     /locus_tag="AC16HB_01850"
     CDS             2003..>4005
                     /locus_tag="AC16HB_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HIR5605561.1"
                     /translation="MRDIREHLPAGYYRLLPKLADGPLRGYPRVYGVAWAIVAHTDSR
                     FDPDWLIRFVHAYQRVQPLTIGELWAVPISLRIVMVENLRRLSVRIVGSQMARQEADQ
                     FVEELRPDIGTRPAELIERAIRSLGSAALWPAFAVHLVQRLRYQAAGTTAMLEWLYRL
                     PATQDLSTDEIVQAQHADQAAANLTVRNLITSMRAIAAFDWRGFFEEVSLVDATLREH
                     EGFAAMDFATRDRYRHAIEDLARGSSCSEIDIAAAVIAKCNPVETDARHGPTLDPRRQ
                     DPGFHLIAAGRPALERELGYRSGLRERCVRACRANAAAVYLGAILLLTLALAALPVIA
                     SIGAGMTWAGLLLLGLLSLLPASDMAIALTHRAVTKIFGARTLPRLALQDGIPPPLRT
                     FVVMPTLLGSTNEIAEHIERLETHYLSNRDGDVFFALLTDWRDADAETVATDGELLAA
                     ATAGIQALNARYGPAEDTGCARFFQLHRARIWNSSERKWMGWERKRGKLHEFNRLLRG
                     ATDTSFLPVDGAAPALPTGVRYVVTVDADTRLPIGAVKRLVGTAAHPMNQPRWDSAAR
                     RVMHGYGVLQPRVTPAFPTRGETTIFQRVFSGPAGLDPYASAVSDVYQDLFGEGSYTG
                     KGLYDVDLFENALAGRVPENTVLSHDLFEGIFARCALVSDVEF"
BASE COUNT          660 a         1335 c         1342 g          668 t
ORIGIN      
        1 cggcacggca acacaagcag gtggcgctgc tgttcctcga tctaaacggt ttcaagcgcg
       61 tcaacgacgg gttcgggcat gcggcaggag accgcatcct gcagcaggtg gcagcgcgcc
      121 tggtcgcttg catccgcacc tccgacaccg cctgccgctt tggcggcgac gaattcgtga
      181 tcctgctgcc ggagctcgaa ggccaggacg gcgcgattgc cgtggcggag aaactgcgcg
      241 cacatctggc cacgccttat gtgctcgacg gaaccgagat ccgattgacc gccagcatcg
      301 gcatggccat ctacccggtc gacgggcacg agtgcgctga tctggtacgg gtgtcggacc
      361 gcgcgatgta tcgcagcaag acccgcgttg cggatgtgcc gggcgaccat gaacccgcgc
      421 tgcaggtgcg gcgtcggccc gctggcttcg aagaatccga ggtgcaccgg catgcaagct
      481 gaccaaccga aacagatccg accgaagacg aagttcgatc gcgcacggga agaggcgttc
      541 agcaacgaag gcgcgccgcc gccgccagac gtacccccga cgggtgcgcc cgatctgcgc
      601 aagcctcagg cccccgcccg gcacgcgcgg tcggcaatgc gcgatccgcc cgcaatggca
      661 ggtagggcag cagtcgaagc actcgcacgc gcgctggtcg acctgtcccc gaccgatcga
      721 ctctattcgt gccgcaccgc cggctggtag gcacgtctct tttgcacgca ccctgactgc
      781 gagccgtccg tgcgctggcg aacagacgcc ccccgctgcg cgccgtatgt tgtgtttgtg
      841 ctttcgatga agggggcagc ctaggagccc cgggcacgat cctgttgagc gtcttgatac
      901 tgattcttct cggcgtcgtt ccgacctgct gaacgcacag tcgatgtggc gcgtcgtgtc
      961 ggcagcgtca agtccctcag gaacgacatg cgggtgaagt gagcgaaagc tgattgcggc
     1021 ccgctgccgg acctcctgtc cggcagcggt cgcgaatgaa gcgatcgcga acgtcgccat
     1081 gaagcgcgcc ggctgagcgg cgacacgcgt gcccgatcgc acggactcta agcgcgcctg
     1141 catccagcgc gtcgttcttt cgtggaggtc tgatgaaact catcctggtc gtttccgcca
     1201 tgttggtcgt gagcgccctt cttctcggca gctgcgcttg attggcgcat acgcgagccg
     1261 gtgggcaccc tcaacgaatg ccggccgatg cgcgcggcgc gtgcatgcgc aatcgacgtc
     1321 gtgcgtcacc atgggagcga acccgcgatc acccgacgtc gcatggaatc gccgcgcagc
     1381 cgacctgccg acgttgccgg gcaccgacac gcggggccgg gcgggaaaat tcatcaccgg
     1441 gtcctgcacg aatcaggccg gcagacgcca atacaggctc tacatcccgg cgcatgacgc
     1501 gcgaccggat ttcgcgctcg tcgtgatgct gcatggctgc gggcacgagc gcgctggaac
     1561 actggctggt gcacggagtg ggccatgcct ggtcgggccg cagtccgagc ggctcgttca
     1621 cggatccgga cggaccgggc gcggcacgcg gaatgatccg attcctcatt caatgcgccg
     1681 gtgacaggtg agcagcagcg cacagctctt ccgccgcctg acccgcaccc gtcgtccgac
     1741 cgacttcacc gaattcgagg agccgatccg cgcggagttg ttcagcgccg aacggctcga
     1801 gcaacacgcg caaagcctgg cggttgcgca aacggtcatc caggatcacg cgggcgagcg
     1861 cgcgctggtt ccgcgcgtgc gggagaacgg ccgcgtattg cacgaagccc atgcgctgat
     1921 cggcgctgcg gcacgacaac gcctcgccat cacgccggcc gctgaatggc tgctcgacaa
     1981 tttccacgtc gtcgaagacc agttgcgcga cattcgcgag catctgccgg ccggctacta
     2041 ccggttgctg ccgaagctcg ccgacggacc gctgcgcggc tatccgcgcg tgtacggcgt
     2101 cgcctgggcc atcgtcgccc ataccgacag ccgcttcgac ccggactggc tgatccgctt
     2161 cgtccacgcc taccagcgcg tgcagccgct cacgatcggg gaactgtggg cggtgccgat
     2221 ttcgctgcgc atcgtgatgg tcgagaacct gcgccgcttg tcggtgcgca tcgtcggttc
     2281 gcagatggcg cggcaggagg cggaccagtt cgtcgaggag ttgcggcccg atatcggcac
     2341 gcgaccggcg gaactgatcg agcgcgcgat tcgcagcctg ggctccgctg cgttgtggcc
     2401 ggcattcgcc gtgcatctgg tgcagcggct gcgttaccag gccgcaggca cgaccgcgat
     2461 gctcgaatgg ctgtaccgct tgcccgcaac gcaggacttg agcaccgacg agatcgtgca
     2521 ggcccagcac gccgaccagg ctgcggcgaa cctcacggtg cgcaacctga tcaccagcat
     2581 gcgcgcgatc gctgcgttcg actggcgcgg tttcttcgag gaagtcagtc tggtcgacgc
     2641 gaccttgcgc gagcacgagg gcttcgccgc gatggacttc gcgacgcggg atcgctaccg
     2701 gcacgcgatc gaggatctcg cgcgtggctc gtcatgttcg gagatcgaca tcgcggccgc
     2761 cgtcatcgcg aagtgcaacc cggtcgagac cgacgcccgc cacggtccca ctctcgatcc
     2821 cagacggcag gatcccggct ttcatctgat cgcggcgggc aggccggcgc tggaacgcga
     2881 actgggttat cgcagcggcc tgcgcgagcg ctgcgttcgc gcctgccgcg cgaacgcagc
     2941 ggcggtgtat ctcggtgcga tcctgctgct gacgctggcg ctggcggcat tgccggtcat
     3001 cgcgagcatc ggggcgggga tgacgtgggc cgggttgctc ctgctcggtc tgctgagcct
     3061 gctgccggct tcggacatgg cgattgctct gacccatcgt gccgtcacga agatcttcgg
     3121 tgcgcggacc ttgccgcggc tggcgcttca ggacggcatc ccgccgccgc tgcggacctt
     3181 cgtcgtgatg ccgaccctgc tcggcagcac gaacgagatc gccgagcaca tcgagcgact
     3241 cgagacgcac tatctgtcga atcgcgacgg cgacgttttc ttcgcgctgc tgacggactg
     3301 gcgcgacgcc gacgccgaga cggtcgccac cgacggcgaa ctgctcgccg cggcgaccgc
     3361 gggaatccag gcgctgaatg cgcgctacgg acccgccgaa gacaccggct gcgcgcgttt
     3421 ctttcaactg catcgcgcgc gcatctggaa ttcgagcgaa cgcaaatgga tgggctggga
     3481 acgcaagcgc ggcaagctgc atgaattcaa ccggctgctg cgcggcgcga ccgatacgag
     3541 ttttctgccg gtcgacggcg cggcgcccgc gttgccgacc ggcgtgcgct atgtggtgac
     3601 cgtcgatgcg gacacgaggc tgccgatcgg cgcggtcaag cggctggtcg gaacggccgc
     3661 gcacccgatg aatcagccac ggtgggacag cgcagcgcgc cgcgtcatgc acggctacgg
     3721 cgtactgcaa ccccgcgtca cgccggcatt tccgacccgg ggcgagacca cgatctttca
     3781 gcgcgtgttt tccggaccgg ccgggctgga tccctatgcg tccgccgtat ccgacgtcta
     3841 ccaggacctg ttcggcgaag gcagctatac gggcaaggga ctgtacgacg tcgatctgtt
     3901 cgagaacgcg ttggcgggcc gggtgccgga aaacaccgtg ctcagccatg acttgttcga
     3961 aggcatattc gcgcgctgcg cactggtgag cgatgtcgag ttctt
//
LOCUS       DBNHBY010000079         1615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1493561, whole genome shotgun sequence.
ACCESSION   DBNHBY010000079 DBNHBY010000000
VERSION     DBNHBY010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1493561"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1474)
                     /locus_tag="AC16HB_01855"
     CDS             complement(65..1474)
                     /locus_tag="AC16HB_01855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5605562.1"
                     /translation="MNAPASLRQFTKPLPSAFLDALKARFGDRCSTAAAVREHHGRDE
                     SPFPPTPPDAVVFAQTTDDVVALTKLCTEHRVPLIPFGVGSSLEGHLLAIEGGVSVDL
                     SQMNRVLAVNAEDLTATVQAGVTRKQLNSEIKDTGLFFPIDPGADATLGGMAATRASG
                     TNAVRYGTMRENVLALTVVTAEGKVIRTARRAKKSSAGYDLTRIFVGAEGTLGVITDV
                     TLKLYPQPEAISAAICNFPTVGDAVRTTIQTIQVGIPIARCELLDAVGVKAVNAYSKL
                     SLRETPLLLFEFHGSPASVREQAEAVQEIAREHHGMDFEWATQAEERSRLWQARHNAY
                     FAVLQLKAGSRALSTDACVPISRLAECIEQTQADLKDSWIISSVIGHVGDGNFHVEML
                     VDPNSATDLEEAERLNRRIVERAIALEGTCTGEHGVGLHKMDFLRIEHGDDALDLMAR
                     IKRAFDPLNILNPGKVVRA"
BASE COUNT          248 a          539 c          551 g          277 t
ORIGIN      
        1 cgcatcgcgc gcgtgggcgc ggtgaccgta tcggagcgca ggtcgatccg ttcgccggtc
       61 atcgtcaagc ccgcacgacc ttgcccgggt tcaggatgtt cagcggatcg aacgcgcgct
      121 tgatgcgcgc catcaggtcg agcgcgtcgt cgccgtgttc gatgcgcagg aagtccatct
      181 tgtgcagccc cacgccgtgc tcgccggtgc aggtgccttc cagcgcgatc gcgcgctcga
      241 cgatgcgccg gttcagccgc tccgcttcct cgaggtcggt cgccgagttc ggatcgacca
      301 gcatctcgac gtggaagttg ccgtcgccga cgtggccgat caccgaactg atgatccacg
      361 agtccttgag atccgcctgc gtctgctcga tgcactcggc caggcgcgag atcggcacgc
      421 aggcgtcagt actcagcgcg cgcgatcctg ccttcagttg cagcaccgcg aaatacgcgt
      481 tgtgccgcgc ctgccacagg cggctgcgct cctcggcctg ggtggcccat tcgaaatcca
      541 tgccgtggtg ctcgcgcgcg atctcctgca ccgcctcggc ctgctcgcgc acgctcgcgg
      601 gcgagccgtg gaactcgaac agcagcagcg gcgtctcgcg cagggagagc ttgctgtacg
      661 cgttgaccgc cttgacgccg accgcgtcga gcagttcgca gcgggcgatc gggattccca
      721 cctggatcgt ctgaatcgtc gtacgcaccg cgtcgccaac ggtcgggaag ttgcagatcg
      781 ccgccgagat tgcctccggc tgcggataca gcttcagcgt gacgtcggtg atcacgccga
      841 gcgtgccttc ggctccgacg aatatgcgcg tcaggtcgta gccggccgac gacttcttcg
      901 cgcgccgcgc ggtgcggatc accttgcctt cggcggtgac gacggtcagc gcgagcacgt
      961 tctcgcgcat cgtgccgtag cgcacggcgt tggtgccgct ggcgcgcgtg gccgccatgc
     1021 cgccgagcgt ggcgtcggcc cccggatcga tcgggaagaa caggcccgtg tccttgatct
     1081 cgctgttcag ctgcttgcgc gtgacaccgg cctgcacggt cgccgtcaga tcctcggcgt
     1141 tgaccgcgag aacgcggttc atctgcgaca gatcgacgct gacgccgccc tcgatcgcga
     1201 gcaggtgtcc ctcgagcgac gaaccgacgc cgaatggaat cagcggcacg cggtgctcgg
     1261 tgcacagttt cgtcagcgcg acgacgtcgt cggtcgtttg cgcgaacacg accgcatcgg
     1321 gcggcgttgg cggaaacggc gattcgtcgc ggccgtgatg ctcgcgcacc gcggccgcag
     1381 tggaacaccg atccccgaag cgcgccttca gcgcgtcgag gaaagcggaa ggaaggggtt
     1441 tcgtgaactg ccgaagcgag gcgggtgcgt tcatggggtc ctcgtgatcc gtgatcggcg
     1501 atccgtgatc cgacgcgcgg gagcggaatc ttagccccga gacggagggc ggcggcataa
     1561 tggccgcatg ggcaaacgac tatcgaagat cgccacgcgc accggcgacg acggt
//
LOCUS       DBNHBY010000080         4747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_275067, whole genome shotgun sequence.
ACCESSION   DBNHBY010000080 DBNHBY010000000
VERSION     DBNHBY010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_275067"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16HB_01860"
     CDS             <1..455
                     /locus_tag="AC16HB_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5605563.1"
                     /translation="GKIFYVADMKSDGVHLVDGESFTKIGFIATGVGAHGLYPSRDGK
                     KLYVANRGSNKVWGPPRGKGSVSVLDFATRKVETTWPILGGGSPDMGNVSADGRTLWL
                     SGRYDDLIYVIDTATGDTKTIASPKEPHGLTVWPQPGRYSLGHTGNLR"
     gene            complement(462..1295)
                     /locus_tag="AC16HB_01865"
     CDS             complement(462..1295)
                     /locus_tag="AC16HB_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5605564.1"
                     /translation="MVARWNDTYFDNTPFAWTGDVAACRAGDTAEAFKLLVLKRLNYY
                     RAMAGLPSDLSLDLAFSAKAQEAALMMDAANSLNHAPATTWPCYSAGGAEAAGRSNLA
                     YSTYPRSVAILDGYMTDRGTNNFAAGHRRWILYSRLATVGTGDAPQANALWVIGSGTT
                     GAVAAKIGVPWPPAGFMPRPLQAPTDRFSYSCAGANFAGATVTMRGDIGQPIATRVES
                     RTDNGYGDNAIVWSIDTAGSPAGGWDRGVADTTLAVDIAGIANCAAGATASYAVTFIT
                     P"
     gene            complement(1638..3008)
                     /locus_tag="AC16HB_01870"
     CDS             complement(1638..3008)
                     /locus_tag="AC16HB_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018713995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR5605565.1"
                     /translation="MVDSVKYTLEEERIPKHWYNLMADLPSPPPPVLHPGTLKPVGPD
                     DLAPLFPLSLILQEVSTEREIEIPAPVREIYKQWRVTPLYRARRLEKALQTPARIYYK
                     YEGVSPAGSHKPNTAVAQAFYNKEAGVKKITTETGAGQWGSSLAFAGAVFGIEVQVFM
                     VRVSYNQKPYRRALMETYGAKCVASPSMETNYGRSVLAKSPDSNGSLGIAISEAVEIA
                     ASRDDTKYALGSVLNHVLLHQTVIGQEAIAQLELANDYPDVIVGCTGGGSNFAGIVFP
                     FLGAQLRGGRKVRVVAVEPSACPSLTRGRYAYDFGDTGHMTPLAKMHTLGSTFMPPGF
                     HAGGLRYHGMSPLVSHIKQLGLIEARAYHQTQCFEAGVFFARHEGIVPAPEANHAVKG
                     AIDEAVRCREEGKSRAILFNLCGHGHFDMQAYLDYFGGKLTDQEYDERELAMALAGLP
                     AVPEPA"
     gene            3114..3323
                     /locus_tag="AC16HB_01875"
     CDS             3114..3323
                     /locus_tag="AC16HB_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HIR5605566.1"
                     /translation="MTNHVYKTIELTGSSPASSDEAIRIAIAKASKTVRDIRWFQVLE
                     TRGQVDSGKVAHWQVTLKVGFTLES"
     gene            3328..4029
                     /locus_tag="AC16HB_01880"
     CDS             3328..4029
                     /locus_tag="AC16HB_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HIR5605567.1"
                     /translation="MTLDRETIDTLARVSTATLTTVLLKKGLRNVWMRGPKPLRPDQP
                     RLVGRAFTLRFIPAREDLATPESWGKPISTRAAIEAMPSGSIAVADAMGVTDAGIFGD
                     ILCARMAKRGVAALITDGVLRDVAGVLATGLPVWCRGVAAPPSVAGLTFVNWHEPIGC
                     GGVAVMPDDVIVVDADGAVVIPAALLDHALAAAPEQERLEAWIMQEVERGAALPGLYP
                     PNEENKARYEASKRR"
     gene            complement(4045..4422)
                     /locus_tag="AC16HB_01885"
     CDS             complement(4045..4422)
                     /locus_tag="AC16HB_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5605568.1"
                     /translation="MIDHLDHIVLTTIDRDACVDFYTRVLGMKLETFAGGRYALRFGM
                     QKINLHERGREFEPKAHLPVPGALDLCFIADRPLDQVIARLAECNWPIIEGPVERTGA
                     TRKLRSVYVRDPDLNLIEISEYV"
     gene            complement(4419..>4747)
                     /locus_tag="AC16HB_01890"
     CDS             complement(4419..>4747)
                     /locus_tag="AC16HB_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5605569.1"
                     /translation="VGTAAWDAITSIFLHLPAKPRRALHERCAAALKPGGVFIYEAYG
                     LEQLRYGTGGPKEPELLPTLDDVLADLNGLRIEHRFAGVRTVQEGRLHHGDGYVLQVV
                     ALREDA"
BASE COUNT          755 a         1597 c         1613 g          782 t
ORIGIN      
        1 acggcaagat cttctatgtc gccgacatga aatccgacgg cgttcatctg gtcgacggcg
       61 aatcgttcac caagatcggc ttcatcgcca ccggcgtcgg cgcgcatggc ttgtacccga
      121 gccgcgatgg gaagaagctc tacgtcgcca atcgcggttc caataaggtg tggggccccc
      181 cgcgcggcaa gggcagcgtc tcggtgctcg acttcgccac gcgcaaggtc gagaccacgt
      241 ggccgatcct cggcggcggc agcccggaca tgggcaacgt cagcgccgac ggccggacac
      301 tttggttgtc gggtcgctac gacgacctga tctacgtgat cgatacggcg accggcgaca
      361 cgaagacgat cgcctcgccg aaggagccgc acggactcac ggtgtggccg cagcccggcc
      421 ggtactcgct cggccacacc gggaacctgc gctgaactcg actacggagt gatgaacgtc
      481 accgcgtagc ttgcggtcgc gccggccgcg cagttggcga tgccggcaat gtcgaccgcg
      541 agcgtcgtgt cggccacgcc gcgatcccag ccgccggccg gagaccccgc ggtgtcgatc
      601 gaccacacga tggcgttgtc gccgtagcca ttgtcggtgc ggctctcgac gcgcgtggcg
      661 atcggctggc cgatgtcgcc gcgcatcgtg accgtggcgc cggcaaaatt cgcgcccgcg
      721 cacgaataag aaaaccgatc cgtcggcgcc tgcagcggcc gcggcatgaa gcccgccggc
      781 ggccacggca caccgatctt cgccgcgaca gcgcccgtcg tgcccgaccc gatcacccac
      841 agcgcgttgg cctgcggtgc atcgccggtg ccgaccgtgg ccagccgcga gtacaggatc
      901 cagcgccgat gcccggccgc gaagttgttc gtgccgcgat cggtcatgta gccgtcgaga
      961 atcgccacgc tgcgcgggta ggtcgaatac gctaggttgg accggccggc ggcctccgcg
     1021 ccgccggcgc tgtagcaagg ccaggtcgtc gccggcgcat ggttcaggct gtttgcggca
     1081 tccatcatca gcgcggcttc ctgcgccttg gcggaaaacg cgagatcgag cgacagatcg
     1141 ctgggcaaac ctgccattgc ccggtagtag ttcaggcgct tgagcaccaa gagcttgaac
     1201 gcttctgccg tgtcgccggc gcggcacgcc gccacgtcgc ccgtccaggc gaacggcgtg
     1261 ttgtcgaagt aggtgtcgtt ccagcgcgcg accacttcgc cacggttgct cgcgtcgatg
     1321 gcgacgacgg gtgcggcgag ctgtccggcc gggggcggtg gagtcggcgt cggcgcgggt
     1381 gccggcgccg gcgtctgtgc actcgagggc tgagacgcac tcgtgctctc cgacccgccg
     1441 cacgcggtga ggacagcgac tggaagcgag gcaatcagca ggaagcgcat cgacgggatg
     1501 agaagggcgc gtcgttccca ccgaacctcg caagaaccgc gcccgtctgc gtgagcccca
     1561 gtccggggcc cgcgaagtat ttcaagctcg tgtccggtac ggcagtcgaa cggaaggact
     1621 accgcgaaag ccgccgccta tgcgggctcc ggaaccgctg gtagtcccgc gagcgccatc
     1681 gccagttcgc gctcgtcgta ctcctgatcg gtgagcttgc cgccgaagta gtccaggtac
     1741 gcctgcatgt cgaagtggcc gtggccgcac aggttgaaca ggatcgcgcg gctcttgccc
     1801 tcctcgcggc agcgcaccgc ctcgtcgatc gcacctttca ccgcgtgatt ggcttcaggc
     1861 gcgggcacga tgccttcgtg tcgcgcgaag aacacacccg cctcgaagca ctgcgtctgg
     1921 tgatacgcgc gcgcttcgat caatccgagt tgcttgatgt gcgacaccag cggcgacata
     1981 ccgtggtagc ggagcccgcc ggcgtggaac cccggcggca tgaaggtcga gccgagcgta
     2041 tgcatcttgg cgagcggcgt catatggccg gtgtcgccga agtcgtatgc gtagcggccg
     2101 cgcgtcaggc tcgggcacgc ggagggctcg acggcaacga cgcgcacctt gcgtccgccg
     2161 cgcagttgcg cgcccaggaa cggaaacacg atgccggcga agttggagcc gcctccggtg
     2221 cagccgacga tcacatcggg atagtcgttc gcaagttcaa gctgcgcgat cgcctcctgc
     2281 ccgatcacgg tctggtgcag cagcacgtgg ttgagcaccg agccgagcgc gtacttggtg
     2341 tcgtcgcggc tggcggcgat ctcgactgcc tcggagatcg cgatgccgag gctgccgttg
     2401 ctgtcgggcg atttcgccag cacgctgcgg ccgtagttcg tttccatcga tgggctggcc
     2461 acgcatttcg cgccgtaggt ttccatcagc gcgcgccggt acggtttctg gttgtacgac
     2521 acacgcacca tgaacacctg cacctcgatg ccgaacacgg cgcccgcgaa cgcgagtgac
     2581 gaaccccact gcccggcgcc ggtctcggtg gttatcttct tcacgccggc ttccttgttg
     2641 tagaaggcct gcgcaacggc ggtgttgggc ttgtggctgc cggcgggact gacgccttcg
     2701 tacttgtagt agatgcgcgc cggcgtctgc agcgcctttt ccaggcgccg cgcccgatac
     2761 agcggcgtca cgcgccactg cttgtagatc tcgcgcaccg gtgcggggat ctcgatctcg
     2821 cgttcggtgg aaacctcctg caggatcagc gacagcggga acagcggtgc caggtcatcg
     2881 ggcccgaccg gcttcagcgt gcccgggtgc agcaccggcg gcggcggcga cggcaggtcc
     2941 gccatcaggt tgtaccagtg cttcgggatg cgctcctctt cgagcgtgta cttgacgctg
     3001 tcgaccatgg tctcctcctc tcgtccgctg ccggctcacg gcgactatag cgccggcacg
     3061 gttgcccgct gcagaggttc gtggcacgct ggccgcacac tcaggaggct gccatgacga
     3121 atcacgtgta caagacgatc gaactcaccg gctcctcgcc ggccagcagc gatgaagcga
     3181 tccggatcgc gatcgcgaag gcgtccaaga ccgttcgcga catccgctgg ttccaggtcc
     3241 tcgagacgcg cggtcaggtc gacagcggca aggtcgcgca ctggcaggtg acgctcaagg
     3301 tcggcttcac gctggaatcc tgacgcgatg acactcgatc gcgagaccat cgacacgctc
     3361 gcacgcgtgt cgaccgcgac gctgaccacc gtgctgctga agaagggtct gcgcaacgtc
     3421 tggatgcgcg ggccgaaacc gctgcggccc gatcagccgc ggctggtcgg ccgcgcgttc
     3481 acgctgcgtt tcatcccggc ccgcgaggac ctcgccacgc ccgagtcgtg gggcaagccg
     3541 atctccaccc gcgcggccat cgaggcgatg ccctccggca gcatcgcggt agccgacgcg
     3601 atgggcgtca ccgacgcggg gatcttcggc gacatcctgt gcgcgcgcat ggcgaagcgc
     3661 ggcgtggcgg ccctgatcac cgacggcgtg ctgcgcgacg tcgccggcgt ccttgcgacg
     3721 gggttgccgg tgtggtgccg cggcgtggct gcgccgccgt cggtggcggg cctgacattc
     3781 gtgaactggc acgagccgat cggctgcggc ggcgtggcgg tcatgccgga cgacgtgatc
     3841 gtcgtcgacg ccgacggcgc ggtcgtgatt cccgctgcgc tcctcgacca cgcgcttgcg
     3901 gccgcgccgg agcaggagcg actcgaggcc tggatcatgc aggaggtcga acgtggtgcc
     3961 gcgctaccgg gactttatcc gcccaacgag gagaacaagg cgcggtacga ggcgtcgaag
     4021 cggcgctgac ggcgcgagcg cgcgtcagac gtactccgag atctcgatca ggttcaggtc
     4081 cggatcgcgc acgtagaccg agcgcagctt gcgggtcgcg ccggtgcgct cgaccgggcc
     4141 ctcgatgatc ggccagttgc attcggcgag ccgcgcgatc acctgatcca gcggccgatc
     4201 ggctatgaag cacaggtcga gcgcgcccgg caccggcagg tgtgccttcg gttcgaattc
     4261 gcggccgcgc tcgtggaggt tgatcttctg catgccgaag cgcagcgcgt agcggccgcc
     4321 cgcgaacgtc tcgagcttca tccccagcac gcgcgtatag aaatcaacgc acgcatcacg
     4381 atcgatcgtc gtcagcacga tgtggtccag atgatcgatc atgcgtcctc cctgagcgcg
     4441 acgacctgca gcacgtaacc gtcgccgtga tgcagccgcc cttcttgcac cgtgcgcaca
     4501 ccggcaaatc ggtgttcgat gcgcagtccg ttcaagtcag cgagcacgtc gtcgagcgtc
     4561 ggcagcagtt ccggctcctt cggcccgccg gtgccgtagc gcagttgttc cagcccgtac
     4621 gcctcgtaga tgaagacgcc gcccggcttc aacgccgccg cgcagcgttc gtgcagcgcg
     4681 cggcgcggtt tcgccggcag gtgcaggaag atcgaagtga tcgcatccca cgcggcggtg
     4741 ccgacat
//
LOCUS       DBNHBY010000081         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_952439, whole genome shotgun sequence.
ACCESSION   DBNHBY010000081 DBNHBY010000000
VERSION     DBNHBY010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_952439"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="feoB"
                     /locus_tag="AC16HB_01895"
     CDS             <1..1437
                     /gene="feoB"
                     /locus_tag="AC16HB_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR5605570.1"
                     /translation="DEARRLGIRIDRDRLARALRAPVHLVDAKHGHGIAPLKEDLRRV
                     IGSAVPAATDAGGATVEDARFEEEAARIAASAIDIPHALPARLTDGVDRVLLHPIWGV
                     PMFFLLVLLLFEVVYGVGLPLQDGLQWLIDASKSVWIEPALASTAPALKSFVLEGVID
                     GVGTVVTFLPIILVFFIAMAVVEDSGYLVRVAFLMDRWMARLGLDGRAFVMQLMGMGC
                     NVPALMGTRVLRSRRQRLLSMLIIPFSLCSARLQVLVFLAAAFFTRQAAPVVMFALYV
                     ASFAVAFGTALVWQRRFRGDEPLVLELPPYRMPTLRSLFAQGWQSSRHFLESASAFIL
                     GGVLLIWWITHYPFDAAPASAGTLAGQLAAWVAPVFAPIGIDAILSIALLFGFVAKEI
                     VLGGLAVIYGAGQDELGRVLADRLTWQQGASFMLFTLIYTPCLSSVATIWRESRSAAF
                     TLLAMLWPLAIAWVASFAFYSVALLFAR"
BASE COUNT          191 a          517 c          570 g          309 t
ORIGIN      
        1 gacgaggcgc gccggcttgg catccgcatc gaccgcgacc ggctggcgcg cgcgttgcgg
       61 gcgccggtgc acctcgtcga cgccaagcat gggcacggca tcgcgccact gaaggaggac
      121 ctgcgccggg tgatcggcag cgccgttccc gcagccaccg atgcgggcgg cgctacggtc
      181 gaggacgcgc gtttcgagga agaggccgcg cggatcgcgg cttcagcgat cgacattccg
      241 cacgcgttgc cggcgcggct gaccgatggc gtcgaccgcg tgttgctgca ccccatctgg
      301 ggcgtgccga tgttcttcct gctggtgttg ctgctgttcg aggtggtcta cggcgtcggc
      361 ctgccgctgc aggacggcct gcagtggctg atcgatgcgt cgaaatcggt gtggatcgag
      421 cccgcgctcg cgtccaccgc cccggcgctg aagagtttcg tgctcgaagg cgtgatcgac
      481 ggcgttggca ccgtggtcac cttcctgccg atcatcctcg tgttcttcat cgcgatggcg
      541 gtggtcgagg acagcggtta tctggtgcgg gtggcgttcc tgatggaccg ctggatggcg
      601 cgcctcggcc tcgatggtcg cgcattcgtg atgcagctga tgggaatggg ctgcaacgtg
      661 cccgcgctga tgggcacgcg cgtgctgcgc tcgcgccggc agcggctgct gtcgatgctg
      721 atcatcccgt tctcgctgtg ctcggcccgg ctgcaggtgc tggtgttcct ggcggcggcg
      781 ttcttcacgc gccaggcggc gccggtcgtg atgttcgcgc tttacgtggc gagcttcgcg
      841 gtcgcgttcg ggacggcgct cgtgtggcag cgccgcttcc gcggcgacga gccgctggtg
      901 ctcgagctgc cgccgtaccg catgccgacg ctcagaagcc tcttcgcgca gggctggcag
      961 tcgtcgcgcc acttcctcga gagcgcgagc gcgttcatcc tcggcggcgt gctgctgatc
     1021 tggtggatca cccactatcc gttcgatgcc gcgccggcca gcgccgggac gctggcgggg
     1081 cagctcgcgg cgtgggtcgc gccggtgttc gcgccgatcg gcatcgacgc gatcctgtcg
     1141 atcgcgctgc tgttcggctt cgtcgccaag gagatcgtgc tcggcgggct tgccgtcatc
     1201 tacggtgccg ggcaggacga actcggccgc gtgctcgcgg accggctgac gtggcagcag
     1261 ggcgcgagct tcatgctgtt cacgctgatc tacacgccat gcctttcgag cgtggcgacg
     1321 atctggcgtg aatcgaggag cgcagccttc acgctgctcg cgatgctgtg gccgctcgcg
     1381 atcgcgtggg tcgcgagctt tgcgttctat tccgtcgcgc tgctgttcgc gcgctgatcc
     1441 gtggccatgc gctgggtcac gccgccgcaa tggggcaact gttacgatcc gccggcgtgc
     1501 cgctgcacgc acccattcca ataagcaggg agagcattcg atgaagcgca ttcatgctgc
     1561 cgcgctggca gctacgctgc tggcaac
//
LOCUS       DBNHBY010000082        12511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1831786, whole genome shotgun sequence.
ACCESSION   DBNHBY010000082 DBNHBY010000000
VERSION     DBNHBY010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1831786"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="AC16HB_01900"
     CDS             complement(<1..554)
                     /locus_tag="AC16HB_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5605571.1"
                     /translation="MFEKVTKTFQYGAHTVRLETGEIARQAGGAVVCSMDDTVVLATV
                     VAQPKAKAGQDFFPLTVDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPL
                     FPDGFFNEVQVVIHVMSVNPEIDPDIPAMIGASAALSISGIPFKGPIGACRVGYIDGA
                     YVLNPTATQLKSTNLDLVVAGTERA"
     gene            complement(756..1025)
                     /gene="rpsO"
                     /locus_tag="AC16HB_01905"
     CDS             complement(756..1025)
                     /gene="rpsO"
                     /locus_tag="AC16HB_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR5605572.1"
                     /translation="MSVADIKKGDIVAKYQRSKSDTGSPEVQVALLTARINELTEHFK
                     THVKDHHSRRGLLKMVSRRRKLLDYLKGRNPESYKGLIDSLGLRK"
     gene            complement(1121..2668)
                     /locus_tag="AC16HB_01910"
     CDS             complement(1121..2668)
                     /locus_tag="AC16HB_01910"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR5605573.1"
                     /translation="MDRLIIFDTTLRDGEQSPGASMTREEKLRIAKQLERLRVDVIEA
                     GFAASSNGDFDAVRAIANTVRESIVCSLARANDKDISRAAAALEGAARKRIHTFIATS
                     PLHMEKKLRMTPDQVFEQAKLSVRFARQFTDDVEFSPEDGYRSEPDFLCRVLEAAIQE
                     GATTINIPDTVGYAVPELYGKFLSDLRTRIPNSDRATWSVHCHNDLGMAVANSLAGVK
                     IGGARQVECTINGLGERAGNCSLEEVVMAVKTRRDFFGLEVGVDTTQIVAASKLVSGI
                     TGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGR
                     NAFKQRVQELAIPVESEAELNAAFAKFKELADRKAEIYDEDIQSLFSDEAVTPHNEHF
                     RLVSLAQRSETGEKPHARIVFSMGAEEVTSESDGNGPVDASLRAIERRVQSGAELLLY
                     SVNAITSGTESQGEVTVRLGRAGRIVNGVGADPDIIAASAKAYLSALNKLYSTAERLN
                     PQTSLAP"
     gene            complement(2866..3699)
                     /gene="pssA"
                     /locus_tag="AC16HB_01915"
     CDS             complement(2866..3699)
                     /gene="pssA"
                     /locus_tag="AC16HB_01915"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR5605574.1"
                     /translation="MRFRRRKPRPFGLAVAPGAGDEIAPPRRRRGIYLLPNLFTLAAL
                     FAGFFAIVQAMNQRFEWAAIAIFVAMVLDGMDGRVARLTHTQSAFGEQFDSLSDMTSF
                     GVAPALVMYEWILNDLGRWGWAGAFVYCAGAALRLARFNANIGVIDKRFFQGLPSPAA
                     AALIAGFVWLAIDNKFHIKDAWMPWVAFGLTVYAGLTMVSSAPFYSGKSFHVARSVPF
                     WVMVVAIVLVFVVSSDPPIALFSLFCCYALSGYLYWGWQWWHGRPNPAKPVQQSLDRS
                     G"
     gene            complement(3699..4337)
                     /locus_tag="AC16HB_01920"
     CDS             complement(3699..4337)
                     /locus_tag="AC16HB_01920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR5605575.1"
                     /translation="MSTYPHPLLAREGWPFVAIAAVAAIVVQALAGFWWALPLWLALL
                     FVVQFFRDPPRDVPAQANAVLAPADGRIVKVDKVRDPYTDRDTLLISVFMNVFNVHSN
                     RSPIDAMVERIDYQGGKFVNADLDKASTENERNAMVLRLDGERVTVVQVAGLIARRIL
                     CYAKVGDRLACGQRYGFIRLGSRVDVYLPLTARPRVVVGDKVAATSTILAEL"
     gene            complement(4392..5111)
                     /locus_tag="AC16HB_01925"
     CDS             complement(4392..5111)
                     /locus_tag="AC16HB_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR5605576.1"
                     /translation="MTPRLLLAAAALVAIAAQAQPTSPPRFEGTLLMMNGVAETEVAN
                     DEAALTFYLEVQEPELARAQSLLNQRVADGVAALKRADPTAQIETSGYGGYPVYQAGS
                     GRKIIGWRARQSVNLKTTALAGLSTTVAAGQQKLALGGIDFRLSRAARERVEGELIQG
                     AIANVNARIAAVAQALNVPASRLRIEELNFGVPTGGRPPPIPVARMSAMASDSVAEPQ
                     LDAGQSMQQVTVAAKVRLLAP"
     gene            complement(5122..6138)
                     /gene="ilvC"
                     /locus_tag="AC16HB_01930"
     CDS             complement(5122..6138)
                     /gene="ilvC"
                     /locus_tag="AC16HB_01930"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5605577.1"
                     /translation="MKVYYDKDADLSLVKGKKVAIVGYGSQGHAHALNLKESGVKVTV
                     ALRKGGASWNKAVNAGLEVKEVGDAVKDADIVMILLPDENHPEVYRQSVEPNIKNGAA
                     LAFAHGFNIHYGQIQPRADLDVIMVAPKAPGHTVRSTYMQGGGVPMLIAVHQNASGAA
                     RDIALSYAAAIGGGKAGVIETNFREETETDLFGEQTVLCGGCVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPEIITAETKAAMKR
                     ALGRIQTGEYAKSFILENRAGATTLHARRRLMAEHEIEKVGEKLRAMMPWIKANRLVD
                     RSRN"
     gene            complement(6253..6744)
                     /gene="ilvN"
                     /locus_tag="AC16HB_01935"
     CDS             complement(6253..6744)
                     /gene="ilvN"
                     /locus_tag="AC16HB_01935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5605578.1"
                     /translation="MRHIISVLLENEPGALSRVVGLFSARGYNIETLTVAPTEDASLS
                     RMTIVTVGSDDIIEQITKHLNRLIEVVKVVDLTEGDYTERELMLIKVRAVGKEREEMK
                     RMADIFRGRIIDVTERTYTIELTGNTEKLDAFIRGVDRASILETVRTGSSGIGRGERV
                     LRV"
     gene            complement(6744..8513)
                     /locus_tag="AC16HB_01940"
     CDS             complement(6744..8513)
                     /locus_tag="AC16HB_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HIR5605579.1"
                     /translation="MQTISAETRTESRPAAPRTEMLTGAEIVVRCLREEGVEHIFGYP
                     GGAVLYIYDAIFKQDSFQHILVRHEQAAVHAADAYSRSSRKIGVALVTSGPGVTNAVT
                     GIATAYMDSIPLVILSGQVPTHAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATI
                     RKAFFIAATGRPGPVLVDIPKDVTIARCEFHYPRDLTLRSYSPVVKGHQGQIKKAVQL
                     LMSAERPMIYTGGGVILSDSSELLNRLVNLLGYPCTSTLMGLGGFKASDPKFMGMLGM
                     HGTYEANQAMQHCDVLLAVGARFDDRVIGNPKHFAQVPRKIIHIDVDPSSISKRVKVD
                     IPIVGDVKEVLTELLTQIEASAQKPAVAPLKAWWTQITEWRKRDCLKYDRSSEIIKPQ
                     FVVEKLWEITKGDAFITSDVGQHQMWAAQYYRFDKPRRWINSGGLGTMGVGLPYAMGV
                     QKANPGAQVAVITGEASVQMCIQELSTCKQYDLTPKICNLNNRYLGMVRQWQQIDYGS
                     RYSHSYVDALPDFVKLAEAYGHVGMVITKPADVEPALREAFGKHKDRLVFLDFITDQT
                     ENVWPMVKAGKGLTEMLLGSEDL"
     gene            8808..9371
                     /locus_tag="AC16HB_01945"
     CDS             8808..9371
                     /locus_tag="AC16HB_01945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5605580.1"
                     /translation="MATREELAEFLASVERRAFKQAVFAVRDDDTALDIVQDAMIKLS
                     ENYGDRPAAELPPLFTRILQNTLHDHFRRQKVRSTWTTLLSSFTPSDDEDADPLETLE
                     ALAGTQAAESAADQVSREQVVGIIEEEVAKLPARQREAFLLRYWEDFDVAETAAAMGC
                     SEGSVKTHCSRATHALARALRERGITL"
     gene            9368..9778
                     /locus_tag="AC16HB_01950"
     CDS             9368..9778
                     /locus_tag="AC16HB_01950"
                     /inference="COORDINATES: protein motif:HMM:NF023699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HIR5605581.1"
                     /translation="MKETEFAYRVRQALNEGAERLDYKTVYRLEQARGRALACHRAGA
                     AEAPVWVPSLQTAGGASAPSSGGFWTWLRRAGLAAPIAALVIGFVSIYQWQRTQQIEH
                     LADLDFAVLLDEGPIDTFADSGFGVMLKSQDHAN"
     gene            9816..10301
                     /locus_tag="AC16HB_01955"
     CDS             9816..10301
                     /locus_tag="AC16HB_01955"
                     /inference="COORDINATES: protein motif:HMM:NF022748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HIR5605582.1"
                     /translation="MAALAATVFFAPAQGQTKAVGKPPATIASRPAWQELSPAQKEAL
                     APLAKDWDGFDRDRKQKWLEVAKKYPSLSADGKQRVHERMSELVKLSPEQRRNVRENF
                     RKAYELPLEQRQALIQEYKDLPPERKRELSAKAKTGSEPAARSTRESRHAKGAKKAEA
                     K"
     gene            10331..10855
                     /locus_tag="AC16HB_01960"
     CDS             10331..10855
                     /locus_tag="AC16HB_01960"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5605583.1"
                     /translation="MHVPFGESSTQSSDPGLLLRLAAMIYEAVLLFSVSFIVVYALLA
                     ALRWTYPLAPYQRWVVQAALFVAFGIYFVYCWTRTGQTLAMKSWHLRLIDRDGRPPPL
                     RTAVLRYLLAWTLFAPGLAFVALFHTHAGWDVLAFAVGFLAMLLLARTHAGRQLLHDR
                     LVGTRIIRDRSRPT"
     gene            complement(10827..11498)
                     /locus_tag="AC16HB_01965"
     CDS             complement(10827..11498)
                     /locus_tag="AC16HB_01965"
                     /inference="COORDINATES: protein motif:HMM:NF053467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8198 family protein"
                     /protein_id="HIR5605584.1"
                     /translation="MTRVIELRRQLEGDTKLRDTWRAVKRWQAERLLQSYADLLAQRR
                     LRPACEFFLAELYGVKDFSQRDQEALRVVPMLARMLPERAVTTMVLAVELDELSEKLD
                     ERLARQIAGTVDDASYAAAYRAAGTRAEREHQIDTIDRIGRSLERLARIPLLAPMLHM
                     MRGPAEMAGLGHLHRFLQSGFDAFVAMGNASDFLDTVRSRETALMQQLFEGGETATSV
                     SIGPG"
     gene            11647..12129
                     /locus_tag="AC16HB_01970"
     CDS             11647..12129
                     /locus_tag="AC16HB_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605585.1"
                     /translation="MKVGFAGGALLAAAGGAAWLLRRGGPDAATSDDTRIVLRAAMPA
                     LLADALPTEPPARAQALEGALARTLATIGGLPLPTRNELGQLFALLASAPGRWLAGVD
                     RWDNADVAQVSAWLQRWRTHTFDLYQVAYHALHDLTLGSYYADPSTWDAIGYPGPIRL
                     "
     gene            12126..>12511
                     /locus_tag="AC16HB_01975"
     CDS             12126..>12511
                     /locus_tag="AC16HB_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5605586.1"
                     /translation="MTAMPPVIADPIAAGLARGWKVVDASTLKAPLQLECDVAVIGTG
                     AGGGISADLFTKSGLNVILVEEGPLASSRDFRMREGDAYPALYWESAARKTKDKAINI
                     LQGRAVGGSTTVNWTSSFRTPPATLA"
BASE COUNT         2100 a         4203 c         4011 g         2197 t
ORIGIN      
        1 gcccgctcgg tgccggcgac gacgaggtcg aggttcgtgc tcttcagctg cgtggcggtc
       61 gggttcagga cgtacgcacc gtcgatgtaa ccgacgcggc aggcgccgat cgggcccttg
      121 aacggaattc cggatatcga cagtgcggcc gaggcgccga tcattgctgg gatgtccgga
      181 tcgatctctg gattcaccga catcacgtga atgacgacct gcacctcgtt gaagaagcca
      241 tcgggaaaca gcggccggat cggccggtcg atcaggcgcg aggtcagggt ttccttttcg
      301 ctcgggcgcc cttctcgctt gaagaagccg cccgggatct tgccggcggc ataggtcttc
      361 tcgacgtaat cgaccgtgag cgggaagaag tcctgccccg ccttggcctt gggctgcgcc
      421 accaccgtcg cgagcacgac cgtgtcgtcc atcgaacaca ccaccgcacc gcccgcctgg
      481 cgcgcgatct caccggtctc cagccgcacc gtgtgggcac cgtactggaa ggtcttcgtc
      541 actttctcga acacttgagc catcctctct gcgtcaactg ttacgcagct gcgacatcgt
      601 tggtcggggc acgccgcggt atcgacgact cggaggtcat ccggccgacc tcctcacgtc
      661 catagcgcgg cgcgcgccag cggaacacgg cggagaatgc aaaaagcccg cggctcaaaa
      721 gctcgcaggc tctatgcatc atctccggga cacgactact tgcgcaggcc gaggctgtcg
      781 atcagcccct tgtacgactc gggattgcgg cccttcaggt agtccagcag cttgcggcgc
      841 cggctgacca tcttgagcag accgcgtcga gagtgatgat ccttgacgtg ggtcttgaag
      901 tgctcggtca gttcgttgat ccgtgccgtc agcagtgcga cctgcacttc cggagatccg
      961 gtgtccgact tgctgcgctg gtacttggcg acgatgtcgc ccttcttgat gtctgcaact
     1021 gacatggttt ccctttcgaa tccacaggcg agcgcggaag gaaggccgcg tcgtgagggg
     1081 cgaagtatac cgcggtgcgg gcttcgcccc ggaacgcgac tcagggcgcc agagacgtct
     1141 gcgggttcag ccgctccgcc gtcgaataca gcttgttcag cgcgctcaga tacgccttgg
     1201 ccgacgccgc gatgatgtcc gggtcggcgc ccacgccgtt gacgatccgt cccgcgcggc
     1261 ccagccgcac ggtcacctca ccctgcgact cggtgccgga ggtgatcgca ttgaccgagt
     1321 acagcagcag ttcggcgccg ctctgaacgc gacgctcgat cgcgcgcagc gacgcgtcga
     1381 ccggaccgtt gccgtcggac tccgacgtga cctcctcggc acccatcgag aacacgatgc
     1441 gcgcgtgcgg cttctcgccg gtttccgacc gctgcgccag cgaaacgagc cggaaatgct
     1501 cgttgtgcgg cgtgaccgcc tcgtccgaga acagcgactg gatgtcctcg tcgtagatct
     1561 cggccttgcg gtcggcaagt tccttgaact tggcgaacgc ggcgttcagt tcggcttcgc
     1621 tttcgaccgg gatcgccagt tcctgcaccc gctgcttgaa cgcgttgcga cccgacagct
     1681 tgcccagcac gatcttgttg gcggtccagc cgacgtcctc ggcgcgcatg atctcgtagg
     1741 tgtcgcgcgc cttcagcaca ccgtcctggt ggatgcccga cgcgtgcgcg aacgcgttgg
     1801 cgccaacgac cgccttgttc ggctgcacga cgaagccagt gatgccggac acgagcttgg
     1861 aggcggcgac gatctgcgtg gtgtcgactc cgacctcgag gccgaagaag tcgcgccgcg
     1921 tcttcaccgc catcacgacc tcctccagcg agcagttgcc cgcgcgctcg ccgaggccgt
     1981 tgatcgtgca ctcgacctgc cgtgcgccgc cgatcttcac accggcgagc gaattggcca
     2041 ccgccatgcc gaggtcgttg tggcagtgga ccgaccaggt tgcccggtcg gagttgggaa
     2101 tccgcgtgcg caggtcggac aggaacttgc cgtaaagctc gggcaccgcg tagccgacgg
     2161 tgtctgggat gttgatcgtc gtggcgccct cctggatcgc cgcctcgagc acgcggcaca
     2221 ggaaatccgg ttccgagcgg taaccgtcct ccggcgagaa ctcaacatcg tcggtgaatt
     2281 ggcgcgcgaa ccgaaccgaa agcttcgcct gctcgaacac ctgatcgggc gtcattcgca
     2341 gcttcttctc catgtgcagc ggcgacgtgg cgatgaaggt gtggatgcgc ttgcgcgccg
     2401 caccctcgag cgcagcggcg gcacggctga tgtccttgtc attggcacgg gccagcgagc
     2461 agacgatcga ctccctgacg gtattggcga tcgcgcgcac ggcgtcgaaa tcgccgttgg
     2521 aactggccgc gaagccggcc tctatcacgt cgacgcgcag ccgctcgagc tgtttggcga
     2581 tgcgcagctt ttcttcgcgc gtcatcgagg cccccgggct ctgctcgccg tcgcgcaacg
     2641 tggtatcgaa gatgatcaag cggtccatgg gcgtctccaa cgtgggtcgg cctgccggca
     2701 ctttgcaggc gggatgttgc agcgggttgt gggggaaggg tcagctgcgc gcggggcgca
     2761 gcagaagcag cagaccgagg tctgcggcgg agcgaaggtc ggcaacggga tgaacagcca
     2821 tgacacggac tataggcgga atcagcggtt gcgacaaccg cgcgcctacc cggaccgatc
     2881 gagcgactgc tgcaccggct ttgccgggtt gggccgtccg tgccaccact gccagcccca
     2941 gtagaggtag ccggacagcg cgtaacagca gaacaggctg aagagcgcga tcggcggatc
     3001 cgacgacacc acgaacacca gcacgattgc gaccaccatc acccagaacg gcacgctgcg
     3061 cgcgacgtga aagctcttgc cgctgtagaa cggcgcgctc gacaccatcg tcagccccgc
     3121 gtaaacggtc agaccgaagg cgacccacgg catccacgcg tccttgatgt ggaacttgtt
     3181 gtcgatcgcc agccagacga agccggcgat cagcgccgcc gcggccgggc tcggcaaccc
     3241 ctggaagaag cgcttgtcga tgacgccgat gttggcgttg aaacgcgcca gccgcagcgc
     3301 ggcacccgcg cagtacacga acgccccggc ccagccccaa cggccgaggt cgttcaggat
     3361 ccattcgtac atcaccagcg ccggcgccac gccgaacgac gtcatgtccg acagggaatc
     3421 gaactgctcg ccgaacgcgc tctgcgtgtg ggtcagccgc gcaacgcggc cgtccatgcc
     3481 gtcgagcacc atcgcgacga agattgcgat tgcggcccac tcgaagcgct ggttcatcgc
     3541 ctgcacgatc gcgaagaatc ccgcaaacaa cgccgccagc gtgaacaggt tcggcaacag
     3601 gtagatgccg cggcggcggc gcggcggcgc gatctcgtcg ccggcccccg gcgcgacggc
     3661 cagtccgaag gggcgcggct tgcggcgacg gaagcgcatc agagctccgc cagaatcgtc
     3721 gacgttgccg ccaccttgtc gccgaccacg acccggggcc gcgcggtcag cggcagatag
     3781 acgtcgacgc gcgagccgag gcggatgaag ccgtagcgct gaccgcacgc gagccggtcg
     3841 ccgacctttg cgtagcacag gatgcggcgc gcgatcaggc ccgccacctg cacgaccgtg
     3901 acgcgttcgc cgtcgagtcg cagcaccatc gcgttgcgct cgttctcggt cgatgccttg
     3961 tccaggtcgg cgttgacgaa ctttccgccc tgatagtcga tgcgttcgac catggcgtca
     4021 atgggcgacc ggttgctatg cacgttgaac acgttcatga acacgctgat cagcagcgtg
     4081 tcacggtcgg tgtacggatc gcgcaccttg tcgaccttga cgatgcggcc gtcggccggc
     4141 gccagtaccg cgttcgcctg cgccggcacg tcgcgcggtg gatcgcggaa gaactgcacc
     4201 acgaacagca gcgccagcca cagcggaagc gcccaccaga atccggccaa cgcctgcacg
     4261 acaatcgcag cgaccgcggc aatcgcgacg aatggccagc cttcgcgcgc cagcaatggg
     4321 tgaggatagg tcgacactga accgagaact cgtatgcgaa agtaccatgg tatcgaagca
     4381 gcgcgcgccg atcagggcgc gagcagacgg accttggccg cgaccgtcac ctgctgcatc
     4441 gactgcccgg cgtcgagctg cggctcggcg accgaatccg acgccatcgc cgacatgcgc
     4501 gcaaccggaa tcggtggcgg gcgccctccg gtcggaacgc cgaagttcag ttcctcgatc
     4561 cgcagccgcg acgccggcac gttcagcgcc tgcgcaaccg cggcgatgcg cgcgttcacg
     4621 ttggcgatcg caccctgtat cagctcgcct tcgacgcgct cgcgcgctgc gcgcgacagc
     4681 cggaaatcga tgccacccag agccagcttc tgctgaccgg ccgcgaccgt cgtcgacaga
     4741 ccggcaagag cagtcgtctt cagattcacg ctctggcgcg cgcgccagcc gatgatcttg
     4801 cggccgctgc cggcctgata caccggatag ccgccatagc cggaggtctc gatctgcgcc
     4861 gtcgggtcgg cgcgcttcag tgccgccacg ccatcggcga cgcgctggtt gagcagcgat
     4921 tgcgcgcgcg ctagttccgg ctcctgcacc tcgagataga acgtcagcgc ggcctcgtcg
     4981 ttcgcgacct ccgtctccgc cacgccgttc atcatcagca gcgtgccttc gaaacgcggc
     5041 ggcgaggtcg gctgcgcttg ggcggcgatc gcgaccagcg cggccgccgc caggaggagg
     5101 cgcggcgtca tgcccaacgg gtcagttccg gctgcggtcg accagccggt tggccttgat
     5161 ccacggcatc atcgcgcgca gtttctcgcc gaccttctcg atttcgtgct ccgccatcag
     5221 acggcgccgg gcgtgcagcg tggtcgcgcc ggcgcggttt tcgaggatga agctcttcgc
     5281 gtattcgcca gtctggatgc ggccgagcgc gcgcttcatc gcagccttgg tctcggcggt
     5341 gatgatctcg gggccggtca cgtactcgcc gtattccgcg ttgttcgaga tcgaatagtt
     5401 catgttggcg atgccgccct cgtacatcag gtcgacgatc agcttcaact cgtgcagaca
     5461 ctcgaagtac gccatctcgg gtgcgtagcc ggcctcgacc agcgtctcga acccggcctt
     5521 gacgagctcc acacagccac cgcacaacac ggtctgttcg ccgaagagat cggtctccgt
     5581 ctcctcgcgg aagttggtct cgatcacgcc cgccttgccg ccgccgatcg ccgccgcgta
     5641 cgacagcgcg atgtcacgcg cggcccccga cgcattctgg tgcacggcga tcagcatcgg
     5701 cacgccgccg ccctgcatgt aggtcgaccg cactgtgtgg cccggcgcct tgggcgccac
     5761 catgatcacg tccaggtccg cgcgcggctg gatctgcccg tagtggatgt tgaatccgtg
     5821 cgcgaacgcg agcgccgcgc cattcttgat gttcggttcg accgactggc gatagacctc
     5881 ggggtggttc tcgtccggca gcaggatcat cacgatgtcg gcatccttca ccgcgtcgcc
     5941 gacttccttg acctccagcc cggcattgac tgccttgttc cacgacgcgc cgcccttgcg
     6001 cagcgccacg gtgaccttga cgcccgattc cttcaggttc agcgcgtgcg cgtgcccctg
     6061 cgacccgtat ccaacgatcg cgaccttttt gcccttgacc agcgacaggt cggcatcctt
     6121 gtcgtagtag accttcatct cttctctcct gaggtttcaa tcattggctt gcccaacctt
     6181 ggctggggga gcacgagggg gccagccgcg ccccttcgct cgtggtggac gcggaacgcg
     6241 ccctcggcgg cgtcagacgc ggaggacgcg ctcgccgcgg ccgatgccgg aacttccggt
     6301 ccgcaccgtt tcgaggatcg atgcacggtc tacgccgcgg atgaacgcgt cgagcttttc
     6361 ggtgttgccg gtcagctcga tcgtgtaagt gcgctcggtc acgtcgatga tgcggccgcg
     6421 gaagatgtcg gccatccgct tcatctcttc gcgctccttg ccgaccgcgc gcaccttgat
     6481 cagcatcagc tcgcgctcgg tatagtcgcc ctcggtcagg tcgaccacct tgaccacctc
     6541 gatcaggcgg ttcaggtgct tggtgatctg ttcgatgatg tcgtccgagc cgaccgtgac
     6601 gatcgtcatg cgcgacaggg aagcgtcctc ggtcggcgcc accgtcagcg tctcgatgtt
     6661 gtagccgcgc gcggagaaga gcccgaccac ccgcgacagc gcgcccggct cgttctccag
     6721 caggaccgaa atgatgtgtc tcatcagagg tcctcgcttc caagcagcat ttcggtcagc
     6781 cccttgcctg ctttcaccat cggccagacg ttctcggtct ggtcggttat gaaatcgagg
     6841 aacaccaacc ggtccttgtg cttgccgaat gcctcgcgca gcgccggctc cacgtcggcc
     6901 ggcttggtga tcaccatgcc gacatgtccg taggcctcgg caagcttgac gaagtcgggc
     6961 agcgcgtcta cgtacgaatg cgaatagcgg ctgccgtagt cgatctgctg ccactgccgc
     7021 accattccca agtaacggtt gttcagattg cagatcttcg gcgtgaggtc gtactgcttg
     7081 caggtcgaca gctcctggat gcacatctgg accgacgcct cgccggtgat caccgcaacc
     7141 tgtgcgcccg ggttggcctt ttgcacgccc atcgcgtacg gaagccccac gcccatcgtg
     7201 ccgagccccc cgctgttgat ccagcgcctc ggcttgtcga accggtaata ctgtgccgcc
     7261 cacatctggt gctggccgac gtccgaggtg atgaacgcgt cgcccttggt gatctcccac
     7321 agcttctcga ccacgaactg cggcttgatg atttcggaag agcggtcgta cttcaggcag
     7381 tcgcgcttgc gccactcggt gatctgggtc caccacgcct tcagcggcgc gacggccggc
     7441 ttctgggctg aagcctcgat ctgcgtgagc agttcggtca gcacttcctt tacgtcgccg
     7501 acgatcggta tatcgacctt cacacgcttc gagatcgacg acgggtcgac atcgatatgg
     7561 atgatcttgc gcggcacctg ggcgaagtgc ttcgggttgc cgatcacgcg atcgtcgaag
     7621 cgcgcgccga ctgccagcag cacgtcgcag tgctgcatgg cctggttggc ctcgtacgtg
     7681 ccgtgcatgc cgagcatgcc catgaacttc ggatccgaag ccttgaagcc gcccagcccc
     7741 atcagcgtgc tggtgcacgg atagccgagg aggttgacca gccggttcag cagctccgac
     7801 gagtccgaca ggatcacgcc gccaccggtg tagatcatcg gccgctcggc gctcatcagc
     7861 agctgcaccg ccttcttgat ctgcccctga tggcctttca cgaccgggct gtacgagcgc
     7921 agtgtcaggt cgcgcgggta gtggaattcg cacctggcaa tcgttacgtc cttcgggatg
     7981 tcgaccagca ccggtcccgg acgaccagtg gccgcaatga agaacgcctt gcggatcgtc
     8041 gccgccagat cgcgcacgtc cttgaccagg aagttgtgct tcacgcacgg ccgcgtgatg
     8101 cccaccgtat cgcactcctg gaacgcgtcc tggccgatcg cgtgcgtcgg cacctgcccg
     8161 ctgaggatca ccaacgggat cgagtccatg taggcggtgg cgatgccggt gaccgcattg
     8221 gtcacccccg ggccgctcgt gaccaaggcg acaccgatct tgcggctgga acgtgagtag
     8281 gcgtcggctg cgtggaccgc ggcctgctcg tgccgcacca ggatgtgctg gaaactgtcc
     8341 tgcttgaaga tcgcgtcgta gatatagaga accgcgccgc cgggataacc gaagatgtgc
     8401 tccacacctt cttcacgcag gcaccgcacg acgatctcgg cgccggtcag catttcggtt
     8461 cggggggccg cggggcggga ttcggtgcgc gtttccgcgc tgatggtctg catttcactg
     8521 ctcctctcaa ggtcctgctg gagattgatt ggatgccgtt ccatatccgc tcgtgacgga
     8581 cggtgcggcc cttcgcgcta acgaatcggc ttctgtggag ccaagcctct tgtgatgcaa
     8641 gaactgtgca cgatactgca gtgcaccagc aagagcaaat gacgcgctga gccgatgcgt
     8701 cgaaacatgc cccgagcgac agcgcgggga agcagacgac cctctgatag catgcgcgcg
     8761 ccctcacccg accgccgggg cgtctcgact cgcgccggca ctcgtggatg gccactcgag
     8821 aagaactcgc cgaattcctc gccagcgtcg aacgtcgcgc cttcaagcag gcggtctttg
     8881 ccgtgcgcga cgacgacacc gcgctggaca tcgtccagga cgccatgatc aagctgtcgg
     8941 aaaattatgg cgaccgtccg gcggccgaac tgccgccgct gttcacgcgg atcctgcaga
     9001 acacgctgca cgaccatttc cggcggcaga aggtgcggtc gacctggacc accctcctct
     9061 cctcgtttac gccgtccgac gacgaggatg ccgacccgct ggaaacgctg gaggcactgg
     9121 ccggaaccca ggctgccgaa agcgccgccg accaggtgtc cagggagcag gtcgtcggca
     9181 tcatcgagga agaagtcgcc aagctgcccg cccgtcaacg agaagctttc ctgctgcgtt
     9241 actgggagga tttcgatgtc gcggaaacgg cggcggcaat gggctgctcg gaaggcagtg
     9301 tgaaaaccca ctgctcgcgt gccacgcatg cgttggcacg cgccctacgc gaacggggaa
     9361 tcacgttatg aaagaaaccg agtttgcata tcgcgtccgt caggccctga acgaaggcgc
     9421 cgaaagactg gactacaaga ccgtttatcg gcttgaacag gcgcgcggcc gggcgctggc
     9481 gtgccatcgc gccggagccg ccgaggcgcc ggtctgggtt ccgtcgctgc agaccgccgg
     9541 tggcgcgtcc gcgccctcgt cgggcggttt ctggacttgg ctgcgccgtg ccgggctggc
     9601 tgctccgatc gccgcactgg tgatcggctt tgtcagtatc taccagtggc aacggaccca
     9661 gcagatcgag catctggccg atctcgactt tgcggtgctg ctggacgagg gtccgatcga
     9721 taccttcgcc gattccggct tcggcgtcat gctgaagtcg caggatcacg ccaactagca
     9781 tgcggacatc catcggactt cgcttctgtc tcgccgtggc cgctcttgcg gccacggtct
     9841 tttttgcacc tgcccagggc cagaccaagg cggtcggcaa accccctgcg accattgcct
     9901 cgcggccggc gtggcaggaa ctttcccccg cccagaagga agcgctggcg ccgctggcaa
     9961 aggactggga cggcttcgac cgtgatcgca agcagaagtg gctcgaggtt gcaaagaagt
    10021 atccgagcct gtcggcggac ggcaagcagc gcgtgcatga acgcatgagc gaactggtca
    10081 agctatctcc ggaacaacgg cgcaacgtgc gcgagaactt ccgcaaggcc tacgagttgc
    10141 cgctcgagca gcgccaggcg ctgatccagg agtacaagga cctgccgccg gagcgcaagc
    10201 gcgagctgtc cgcaaaagcg aagaccggca gcgagcccgc cgccaggtcg acgcgcgaat
    10261 cgcggcacgc caagggcgcc aagaaggcag aagcaaagta acgggcacag cgaccgactg
    10321 tccccggccg gtgcacgtgc ccttcggcga atcgtctacc caatcgtcgg atcccggcct
    10381 gctgctgcgc ctggccgcaa tgatctacga agcggtgctt ctgttcagcg tttcgttcat
    10441 cgtcgtctac gcgctgcttg ccgcgttgcg ctggacctat ccgctggcgc cgtaccagcg
    10501 ctgggtggtg caggcggcac tgttcgtcgc gttcgggatc tacttcgtgt actgctggac
    10561 gcggaccgga cagacgctgg ccatgaagtc atggcacttg cgactgatcg accgcgacgg
    10621 ccgcccaccg ccgctgcgca ccgctgtgct gcgctacctg ctggcgtgga cgctgttcgc
    10681 gcccgggctg gcgttcgtcg cgctgttcca cacgcatgcg gggtgggacg tgcttgcgtt
    10741 cgcggtcggc ttcttggcga tgctgctgct cgcgcgaaca catgccggtc ggcaactgct
    10801 gcacgatcgg ctcgtcggca cgcgcatcat ccgggaccga tcgagaccga cgtagccgtc
    10861 tcgccaccct cgaagagctg ctgcatcagc gcggtctcgc ggctgcggac cgtatcgagg
    10921 aaatcgctcg cgttgcccat cgcaacgaag gcgtcgaagc ccgactgcag gaagcggtgc
    10981 aggtggccga gccccgccat ttccgccggc ccgcgcatca tgtgcagcat cggcgccagc
    11041 agcggaatgc gcgccaggcg ctcgagcgaa cggccgatgc gatcgatggt gtcgatctgg
    11101 tgttcgcgct cggcgcgcgt gccggccgcg cgataggcgg ccgcgtagct cgcatcgtcg
    11161 acagtgcccg cgatctggcg cgcgaggcgc tcgtcgagtt tctcggacag ctcgtcgagc
    11221 tcgaccgcaa ggaccatcgt cgtcactgca cgctcgggca gcatgcgcgc cagcatcgga
    11281 accacgcgga gcgcttcctg atcgcgctgc gagaagtcct tgaccccgta gagctcggcg
    11341 agaaagaact cgcacgcggg gcgcagccgc cgttgcgcca gcaggtcggc gtatgactgc
    11401 aacaggcgct cggcctgcca gcgtttcacc gcgcgccagg tatcgcgcaa cttcgtgtcg
    11461 ccttcgagct ggcggcgcaa ttcgatgacc cgcgtcaatg cagcggcgag tcggtcggcc
    11521 ggtgacttcg tttgatccat cgcagccact gggtttagac gggcagttct acggcgcctt
    11581 tctacaatgg ccggcagaat acgtgaggac ttcgcggttc ggtgtgacat gcggcgacgc
    11641 acgctcttga aagtcggatt tgccggcggc gcgctgctcg cggccgcagg tggcgcggcc
    11701 tggctgctcc ggcgcggcgg accggatgcc gcgaccagcg acgacacgcg catcgttctg
    11761 cgcgctgcga tgccggcatt gctcgccgac gcgctgccga cggaaccgcc tgcacgcgcg
    11821 caggcgctcg aaggcgcgct tgcgcgcacg ctggcgacga tcggcggcct gccgctgccg
    11881 acgcgcaatg agctcggcca actgttcgcg ctgctcgcct cggcgcccgg gcgctggctg
    11941 gccggcgtcg accggtggga caacgcggac gtggcgcagg tcagcgcgtg gctgcaacgc
    12001 tggcgtacgc acacgttcga cctctaccag gtcgcctacc acgcactgca cgacctgacg
    12061 cttggctcct actacgccga tccatcgaca tgggacgcga tcggctaccc cgggccgatc
    12121 aggctatgac cgcaatgcca cccgtgatcg ccgacccgat cgccgcgggg ctcgcacgcg
    12181 gctggaaggt cgtcgacgca tcgacgctca aggcaccgct gcagctcgaa tgcgatgtcg
    12241 ctgtgatcgg caccggcgcc ggcggcggca tcagcgccga tctgttcacc aagtcagggc
    12301 tgaacgtgat cctggtcgag gaaggaccgc tcgcgagttc acgcgacttc aggatgcgcg
    12361 aaggcgatgc ctatcctgcc ctctactggg aatccgcagc gcgcaagacc aaggacaagg
    12421 cgatcaacat cctgcaaggg cgcgcggtcg gcggctcgac gacggtgaac tggacctcga
    12481 gtttccgcac cccgccggcg acgctcgcat a
//
LOCUS       DBNHBY010000083         2239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_275322, whole genome shotgun sequence.
ACCESSION   DBNHBY010000083 DBNHBY010000000
VERSION     DBNHBY010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_275322"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1175
                     /gene="scpB"
                     /locus_tag="AC16HB_01980"
     CDS             396..1175
                     /gene="scpB"
                     /locus_tag="AC16HB_01980"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HIR5605587.1"
                     /translation="MSFAQIETRNQIGIITLAHPERRNALSKALVDEIIAALAAFKAS
                     RLRAVILRAQPGAKVWSAGHDVDELPTSGRDPLGWDDPLRHLIREIETCPAPVIAMIE
                     GTVWGGACEAVLACDLIVATPESTFAITPARLGVPYNIGGVLTFLNATSLRIGKEMAF
                     TAKPISAERAEQHGMINYVVPVEKLEPFTFDLAATICENAPLSIAVMKEQMRILAGAH
                     PMSPQGFERVQGLRRIVYDSGDYQEGIRAFKEKRRPVFRGE"
     gene            complement(1115..>2239)
                     /locus_tag="AC16HB_01985"
     CDS             complement(1115..>2239)
                     /locus_tag="AC16HB_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605588.1"
                     /translation="GAVSPAHAQISVGIAVPGISIGINLPVYPELVPVPGYPVYYAPR
                     VNSNFFFYDGLYWVFQGDNWYASSWYNGPWHMTAPEYVPVFVLRIPVRYYRQPPVYFR
                     DWRRDAPPRWGEHWGRDWEQRRSGWDQWDRRSAPRPAPLPVYQRQYSGNRYPSAVEQQ
                     HAIRSERYRYQPREAVTRQYFQQQGSARAEPRQQGPGQQRPSAQQQRSQPPQQRQQAQ
                     PMQPPPQMQHPQAERQRQEQGQGKGQGKGREYKGEPQGKGQDSKRDERRQDHRESRRF
                     DEPARRRSESNRPRSGRRRRREQAVVFLADHGKVLSADVQGTTKPSTDTLSAANRLES
                     ASVSKCGSASCEITQRRGAARYSPRNTGLRFSLKARMPSW"
BASE COUNT          374 a          724 c          716 g          425 t
ORIGIN      
        1 gcgattcccg accgcaacga aagccgacat tcgatcggcg gtccgatttc gattgctcgt
       61 cgtcgaagcc caaagcggtc gttggtgatt ctcgcgaacg tcggcccgct ccacctgcag
      121 gccgctatcc catccttacc cgggacgcga atttccatcg gaaactcagc agcggccgcg
      181 accgacggat catggtcggc taccgccccc tgcgctgcat cggtcgattg cggtcgcagc
      241 gtaatctttc acggccggcg aagcgctgcc ctcgcgaagt gcacgtggtt gcgccgcccg
      301 ggtcctgatg tgcccgcagc gcgccaccac tcagggggcg gcaagcgtca cctagctgga
      361 ccgcgcacct tagtttcgac caattcggag acgccatgtc gttcgcgcag atcgagaccc
      421 gcaatcagat cggcatcatc acactcgctc accccgagcg acgcaatgct ttgtccaagg
      481 cgctggtcga tgaaatcatc gccgcgctgg ccgccttcaa ggcaagccga ctgcgcgcag
      541 tgatcttgcg cgcccagcct ggcgcgaagg tctggtcggc cgggcatgac gtcgacgaac
      601 tccccacgtc gggacgcgat ccactcggct gggacgatcc gttgcgtcac ctgatccgcg
      661 agatcgaaac ctgtccggca ccggtgattg cgatgatcga aggcacggtc tggggcggcg
      721 cctgcgaggc ggtgttggcg tgcgacctga tcgtggcgac gccggaatcc accttcgcca
      781 tcacgccggc gcggctgggc gtgccgtaca acatcggcgg cgtgctgacc tttctgaacg
      841 cgaccagcct gcgcatcggc aaggaaatgg cattcaccgc caaacccatc agcgccgagc
      901 gggccgagca gcacggcatg atcaactacg tggtgcccgt cgagaagctg gagccattca
      961 ccttcgacct tgcggcgacc atctgcgaga acgcgccgtt gtcgatcgcg gtgatgaagg
     1021 agcagatgcg tatcctcgcc ggcgcgcacc cgatgtcgcc gcagggattc gagcgcgtgc
     1081 aggggctccg gcggatcgtc tacgacagcg gcgattacca ggaaggcatc cgcgccttca
     1141 aggaaaagcg caggccggtg tttcgcggcg aatagcgtgc ggcgccgcgt cgctgtgtga
     1201 tctcgcaact cgctgatccg cacttcgaga ccgacgccga ttccagtcgg ttggcggcag
     1261 acagcgtatc ggtggacggc ttagtcgttc cctgaacgtc ggcgctcagg accttgccgt
     1321 ggtcagcaag gaatacgact gcctgctctc ggcgacgacg gcgacctgag cgcggtcgat
     1381 tggattcaga gcgccgtcgc gcaggctcat cgaacctgcg cgattcccgg tgatcctgac
     1441 ggcgctcatc cctcttgctg tcctgaccct tgccttgcgg ttcgcccttg tactcgcgac
     1501 ccttgccttg tccctttccc tgtccttgtt cctgtctttg cctttcagct tgggggtgct
     1561 gcatttgcgg cggcggctgc atgggttgcg cctgttgccg ctgctgcgga ggttgcgatc
     1621 gctgttgctg ggccgagggg cgctgctgac ccggcccctg ctgacgcggc tcggcgcgcg
     1681 cgcttccctg ctgctggaaa tactgccgcg tcacggcctc acgtggctgg tagcgatatc
     1741 tttcggatcg aatcgcgtgt tgttgttcta ctgcgctcgg ataccggttc cccgaatatt
     1801 gccgctgata taccggtaac ggagcgggac gaggagcgga acgacggtcc cattggtccc
     1861 atccgcttcg gcgctgttcc cagtcgcggc cccagtgctc gccccagcgt ggcggggcgt
     1921 cgcgacgcca gtcgcggaag tagaccggag gctggcggta gtagcgcacg ggaatgcgca
     1981 gtacgaacac cggcacatac tcgggcgccg tcatgtgcca cggaccgttg taccaactgc
     2041 tcgcatacca gttgtctccc tggaacaccc agtacaggcc gtcatagaag aagaagttcg
     2101 agttcactcg cggcgcgtag tagacgggat agcccggaac tggaacgagt tccggataca
     2161 cgggaaggtt gataccgatg ctgatgccag gaactgcaat gccgacgctg atctgggcgt
     2221 gggctgggct gacggcgcc
//
LOCUS       DBNHBY010000084         1701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_749241, whole genome shotgun sequence.
ACCESSION   DBNHBY010000084 DBNHBY010000000
VERSION     DBNHBY010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_749241"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16HB_01990"
     CDS             complement(<1..476)
                     /locus_tag="AC16HB_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HIR5605589.1"
                     /translation="MPWVGALFIAIIVAMAAFDIVRGYRMTVEDTGRELETQARIVAE
                     QTARSVQAIDVVLRHIAGEYRRGRLARLNPEELHAYLVEQSVGLVQIAGLAMHDANGD
                     SLAMSWLAPRTDLNIAHLPGFQQLRDDPKADLVIGMATRSASDGQWVIPIGRRLESP"
     gene            complement(530..892)
                     /locus_tag="AC16HB_01995"
     CDS             complement(530..892)
                     /locus_tag="AC16HB_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605590.1"
                     /translation="MIAPAPLIRVVNADRATLGLLQEWLDAAGYRVADGATTKEAAQL
                     TIVDVPFTRHGGLELLKRVAGEAPGVPILALSATFFSNVRCGGDCARSLGVAGVLPKP
                     LARDALVNAVDGLLRPAA"
     gene            1166..>1701
                     /locus_tag="AC16HB_02000"
     CDS             1166..>1701
                     /locus_tag="AC16HB_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5605591.1"
                     /translation="MHGPTPNEAPSSATTGATTQPHVLALDDDTSVRSLIAEYLTQHD
                     LRVTTVASGRELAAAMARETFDLVVLDLRLPGEDGMQIAQKLRAESAIPILMLTGRVD
                     EADRVMGLELGADDYLTKPFSPRELLARIRALLRRAQAGATVADAISKVRAYRFGNWE
                     LSIGLRRLKSADGRTLELT"
BASE COUNT          281 a          598 c          566 g          256 t
ORIGIN      
        1 ggcgactcga gccggcgccc gatcgggatc acccattgcc cgtcgctcgc gctgcgcgtg
       61 gccatgccga tcacgaggtc cgccttcggg tcgtcgcgca gctgctggaa gccgggcagg
      121 tgtgcgatgt tcaggtccgt gcgcggtgca agccaggaca tcgccagcga atcgccgttg
      181 gcgtcgtgca tggcgagccc ggcaatctgc accagtccga ccgactgctc gacgaggtac
      241 gcgtgcagtt cctccgggtt caggcgcgcc agccggccgc gccggtattc gccggcgatg
      301 tggcgcagca ccacgtcgat cgcctgcacg ctgcgcgcgg tctgttcggc gacgatgcgg
      361 gcctgcgtct cgagttcgcg tccggtgtcc tcgaccgtca tccgatagcc gcgaacgatg
      421 tcgaacgcgg ccatcgcgac gatgatcgcg atgaacagcg cccccaccca cggcacgctc
      481 cgacgcctcc agggtcggcc cccgaaagcg tcgaaagcgt tggcgagcct catgccgccg
      541 gccgcagcaa cccgtcgacc gcgttgacca gcgcgtcgcg cgccagcggt ttgggcagca
      601 cgccggcgac gccgagcgaa cgtgcgcagt caccgccgca gcggacgttc gagaagaacg
      661 tcgccgacag agcaagaatc ggcaccccag gggcctcgcc cgccacgcgc ttgagcagct
      721 cgagcccgcc gtggcgcgta aagggcacgt ccacgattgt cagttgcgcc gcttccttcg
      781 tggtggcccc atcggcgacc cgatagccgg ccgcgtccag ccattcctgc agcaatccga
      841 gcgtggcgcg gtccgcgttg accacgcgga tcagcggcgc gggggcaatc atcggtgcgg
      901 cccctgcagc ccgagcgacg accacggcga ttgcatctgt gtctctccca atggatgatt
      961 cgaacgggcc gcgccactca ccgcgccgaa ccccctccgc agtcgactgc ggatggcgtc
     1021 tattgggcaa ccgtaccgtt gccccgccat ttccggcaag catgggtgaa ttacatttgt
     1081 aattgcggct gtgccaacgg cggatgcgcg ctgccgcgag ttatgagaca gtaaagcgat
     1141 ccgcgaggtg ccggaggaga tattcatgca cggaccgacg cccaacgaag cgccgtcgag
     1201 cgcgacaacg ggcgcgacga cccagccgca cgtgctggcg ctcgatgacg ataccagcgt
     1261 ccgctcgctg atcgccgagt acctgaccca gcacgatctg cgcgtgacca cggtcgcgag
     1321 cggccgtgaa ctggccgcgg cgatggcgcg cgagacgttc gacctggtcg tgctcgatct
     1381 gagattgccc ggcgaagatg gcatgcagat cgcgcagaag ctgcgcgccg agtccgcgat
     1441 cccgatcctg atgctgaccg ggcgagtcga cgaggccgac cgggtgatgg ggctcgaact
     1501 cggcgccgac gactacctga ccaagccgtt ttcgccgcgc gaactgctcg cacgcatccg
     1561 cgcactgctg cggcgcgcgc aggcgggcgc caccgtcgcc gacgccatct cgaaggtgcg
     1621 cgcttaccgc ttcggcaact gggaactttc gatcggcctg cgacggctga agtccgccga
     1681 cggcaggacg ctcgaactca c
//
LOCUS       DBNHBY010000085         6211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1764186, whole genome shotgun sequence.
ACCESSION   DBNHBY010000085 DBNHBY010000000
VERSION     DBNHBY010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1764186"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC16HB_02005"
     CDS             <1..507
                     /locus_tag="AC16HB_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR5605592.1"
                     /translation="RTEAPRTHMFLTVPLPDGVFVVDPGFGSFAPRVPVPLRDRVPAR
                     RGAEVHWLAFDDGRWTLCAQSGDAVVEAWVSTLEEEYPIDFELGNHYTSTHPDSGFRH
                     HIMLRALTPDGRIGVLNRDVTHWRDGTPHKFRLADRAELRALLAEHFGFDLPEVDRLR
                     VSSIPEWS"
     gene            complement(504..2126)
                     /locus_tag="AC16HB_02010"
     CDS             complement(504..2126)
                     /locus_tag="AC16HB_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HIR5605593.1"
                     /translation="MTSDAQVVVRHFRQILLWPLQLMPIRPGAQIQKHWEHLDRTHAE
                     HPWRRVVDEIGNPDAFEERHYSEFITFLPHVQRFLYGEGDGGGAGDSPIRAYRRSDIA
                     AVRVTLSAAHAQPVEFALARVDLYFFLDLDVVILALEMHAADVPLDLAQEALYRFGRA
                     YPPFWEDDGSGGHCPRRVEWLDADGRLLAASDYEDRERYLHSVATHRAPEIASHWEFL
                     LAPMVQNHLPRSGALRFRQLEYYRMPLMAYFAVDEPQKLTRAQFVRLALATGEGQADD
                     LPYSAQHLADFERRYCYDRGWRDAAPTRLMSCGHATVVVGSSRDRYFTGRERGALAQF
                     RHQQFLVVLIAHLHKAALLMLSDRLVGALNRLDIASFESVKQFKRTIRATKEIFLRFS
                     HRYWFHEVSDQMLARDLHHLCAGHLGVEQLYTEVRDEIQDMSDYLDSDSLRRQANTVV
                     RLTVVTTFGLIGTVVTGFLGMNLFALAEASTAAKLAYFVLTLVPVTVLTFYTIVKSKR
                     LSDFLEALSDERLPARAKAASLAAVWQRGGAA"
     gene            complement(2209..2457)
                     /locus_tag="AC16HB_02015"
     CDS             complement(2209..2457)
                     /locus_tag="AC16HB_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605594.1"
                     /translation="MVDPDEGSTRRFLSAPIQVGRGELPSLRAAPGFCLGAAVNELIG
                     IGGTHAPLFAAVDAARASPAGPRHRRGPESPGRGPTML"
     gene            complement(2457..2732)
                     /locus_tag="AC16HB_02020"
     CDS             complement(2457..2732)
                     /locus_tag="AC16HB_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR5605595.1"
                     /translation="MPRYLIERTFPDGLSIPMNADGAKACMGVVANNAEKGVNWLHSY
                     VTPDRKHTYCIYDGPTPEAIRSVAQRNGLPVDRITEVSVLDPYFYRP"
     gene            complement(2828..5419)
                     /locus_tag="AC16HB_02025"
     CDS             complement(2828..5419)
                     /locus_tag="AC16HB_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5605596.1"
                     /translation="MQLLERAPLLETLNRCFERARDGRGVMVFIAGEAGIGKSALVSH
                     FAESQREPARVLWGNCEAMSTPTPLGALQDIALQLADAGSPLPPLDVPRIDLFAACLN
                     ALRGAPTVAVFEDVHWADAATLDLLRYFGRRAERTRALLIATYRDDELGPSHPLRVVL
                     GDLASAGVQRLTIPPLTADAVRTLAGSRSIDAAALHAATGGNAFFVTEALGAGGSGIP
                     PTVRDAVLARAARLTPSARAVLEYAAVAGPRVEPWLLAHLAAAEADAITQCEASGMLR
                     IDAGRYTFRHELARQAILESLAPARRVSMHEAVLLALRTAPSTQTDLARLAHHANLAE
                     DADAVLHFAPLAARQAAARGAHREAAAQYAAALQYADRLEAGQRAALIEARAYECYLI
                     SDIEAAIAARQVALQLARATGNALREGENLRWLSRLHWFLGHGEDAERCGAAAVQVLQ
                     PLPHGPELAMAFSNLSQLKMLAEDVDAAVAWGRRAIDLAERLDATEILAHALNNVGAS
                     LDRIGDPAGAPQLERSLALALEHGLEEHAARAYCNLASHAISQREYGRAEPYLAAGIA
                     YCADRDLDSWSHYLMAWRTRSRFDQGRWDEAALEAAWLCERSGAAPVARIPALAVLGH
                     VRVRRGDPAAAAALDEGHALACSSGELQRIWPVAVARAEAAWLQGDAQRCVAEVRTCF
                     ELARLRRNPWALGETGVCLRRAGALAEVPDGCAAPFALQLRGEWRAAAAAWQALGCPY
                     EQACALADGDAAAQIEALAIFDRLGAAPAARRLREAMRACGERSIPRGPRAATRANRF
                     GLTPRELDILALLAHGLSNAQIAGRRHRSVRTVDHHVASILAKLGAVTRNEAVAQARA
                     HDLLTEK"
     gene            complement(5576..>6211)
                     /locus_tag="AC16HB_02030"
     CDS             complement(5576..>6211)
                     /locus_tag="AC16HB_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5605597.1"
                     /translation="LRTIEMNFLPDVKVLCDVCGGRRFNRETLGIQMRGKTAGEVLAM
                     EVDEAVPFFGAHPSIARPLQLLQDVGLGYLTLGQPSPTLSGGEAQRIKLVTELAKVRM
                     DEGLVRTGRAYKPLHTLYVLDEPTVGLSMHDVEKLLAVLHRLVGAGNTVVVIEHNLDV
                     IAEADWIVDLGPEGGDGGGRVIAQCPPQQLARRDTHTGHALREFAAGRGTV"
BASE COUNT          903 a         2221 c         2186 g          901 t
ORIGIN      
        1 cgcaccgagg cgccgcgcac ccacatgttc ctgaccgtgc cgctgccgga cggcgtcttc
       61 gtggtcgatc ccggcttcgg ctcgttcgcg ccgcgcgtgc cggtgccgct gcgcgaccgc
      121 gtgccggcgc ggcgcggggc cgaagtgcac tggctggcgt tcgacgacgg gcgctggacg
      181 ctgtgtgcgc agtcgggcga cgcggtggtc gaggcctggg tgtcgacgtt ggaggaggag
      241 tacccgatcg acttcgagct cggcaaccac tacacatcga cgcatccgga ttcgggattc
      301 cggcatcaca tcatgctgcg cgcgctgacg cccgacggcc gcatcggcgt gctgaatcgc
      361 gatgtcacgc actggcgtga cggcacgccg cacaagttcc ggcttgccga tcgggccgaa
      421 ctgcgcgcgc ttctggccga gcacttcggc ttcgacctgc cggaggtcga tcggctgcgc
      481 gtgtcgtcga taccggagtg gtcctaggct gcgcccccgc gctgccacac cgcggccagc
      541 gatgccgcct ttgcgcgcgc cggcaggcgc tcgtcggaca gcgcctcgag gaagtccgac
      601 agccgcttcg acttgacgat cgtgtagaag gtcagcacgg tcaccggcac cagcgtcagc
      661 acgaagtacg ccagcttggc cgcggtgctc gcctccgcga gtgcgaacag gttcatcccg
      721 aggaatccgg tcaccacggt gccgatcagc ccgaacgtgg tgaccacggt cagccgcacg
      781 accgtgttgg cctggcgccg cagcgagtcg ctgtcgaggt agtcgctcat gtcctggatc
      841 tcgtcgcgca cctcggtgta gagctgctcg acgccaaggt ggccggcgca caggtggtgc
      901 aggtcgcgcg cgagcatctg gtccgacact tcgtggaacc agtagcggtg cgagaagcgc
      961 aggaagatct ccttggtcgc gcggatcgtg cgcttgaact gcttgaccga ttcgaacgag
     1021 gcgatgtcga ggcggttcag cgcgccgacc agccggtccg acagcatcag cagcgccgcc
     1081 ttgtgcaggt gggcgatcag gacgaccagg aactgctggt gccggaactg cgccagcgca
     1141 ccgcgctcgc gtccggtgaa gtagcggtcg cggctcgaac cgacgacgac ggtcgcatgg
     1201 ccgcagctca tcaagcgcgt cggcgccgcg tcgcgccagc cgcggtcgta gcagtagcgg
     1261 cgctcgaagt ccgcgaggtg ctgcgcggaa tacggcaggt cgtcggcctg gccctcgccg
     1321 gtggcgagcg ccagccgcac gaactgtgcg cgcgtcagct tctgcggctc gtcgacggcg
     1381 aagtacgcca tcagcggcat ccggtagtac tcgagctggc gaaagcgcag cgccccgctg
     1441 cgcggcagat ggttctgcac catcggcgcc agcaggaact cccagtgcga cgcgatctcc
     1501 ggcgcgcgat gggtggccac cgagtgcaga tagcgctcac ggtcctcgta gtcggacgcg
     1561 gcgagcagcc ggccgtccgc gtcgagccat tcgacgcgcc gcggacaatg gccgccgctg
     1621 ccgtcgtcct cccagaacgg cggatacgcg cgaccgaagc ggtagagcgc ctcctgcgcc
     1681 agatcgagcg gcacgtccgc ggcgtgcatc tcgagcgcga ggatcacgac atcgaggtcg
     1741 agaaagaagt acaggtcgac acgggcgagc gcgaactcga ccggctgcgc gtgcgcggcc
     1801 gacagcgtca cgcgcacggc ggcgatgtcg ctgcgacgat aagcgcggat cggcgaatcg
     1861 ccggcgccgc cgccgtcgcc ttcgccgtac aggaagcgct gcacgtgcgg caggaaggtg
     1921 atgaactcgc tgtagtgccg ctcctcgaac gcgtccggat tgccgatctc gtcgaccacg
     1981 cgccgccacg ggtgctcggc gtgcgtgcgg tcaaggtgtt cccagtgctt ctggatctgc
     2041 gcaccgggcc ggatcggcat cagctgcagc ggccacagca gaatctgtcg gaagtgccgc
     2101 accacgacct gcgcatcgct tgtcatcgct gcctccctcg cgcgggccga ttgtgctgcg
     2161 cggaggagcg acgctcaagc atgcatgccg gcggcgagcg ggcgttcgtt acagcatcgt
     2221 cggtcccctg ccgggagatt cggggccacg tcggtgccgc ggaccggcgg gcgaagcgcg
     2281 cgcggcatcg accgccgcga acagcggagc atgcgtgccg ccgatgccga tcagttcgtt
     2341 cacggcggcg ccgaggcaga agcccggcgc cgcgcgcagc gaaggcagct cgccgcggcc
     2401 gacctgaatc ggtgcggaca ggaagcggcg agtgctgcct tcgtctggat cgaccatcag
     2461 ggccgataga agtacggatc gagcaccgag acctcggtga tgcgatcgac cggcagcccg
     2521 ttgcgctggg ccacgctgcg gatcgcctcg ggcgtgggcc cgtcgtagat gcagtacgtg
     2581 tgcttgcgat cgggtgtcac gtacgaatgc agccagttga cgcccttctc ggcattgttg
     2641 gcgaccacgc ccatgcaggc cttggcgccg tcggcattca tcggaatcga cagtccgtcg
     2701 gggaaagtgc gttcgatcag gtaacgcggc atgggacctc ctttgtgtgt gaaccgcggc
     2761 cacacggtag gccgcggcgc gccgcgcagc atcggcggcc gctgcccata tttcgtgccc
     2821 cgattgccta tttttcggtg agcagatcgt gcgcgcgggc ctgcgcgacg gcctcgttgc
     2881 gcgtcaccgc gccgagcttg gcgaggatcg atgcgacgtg atggtcgacc gtgcgcaccg
     2941 agcggtggcg gcgtcccgca atctgcgcgt tcgacagccc gtgcgcgagc agcgccagga
     3001 tgtcgagctc gcgcggcgtc agtccgaagc ggttcgcgcg cgttgccgcg cgcgggccgc
     3061 gtgggatcga gcgctcgccg caggcacgca tggcctcgcg cagccgccgc gccgccggcg
     3121 ctgcgccgag ccggtcgaag atcgccagcg cctcgatctg cgccgccgcg tcgccgtcgg
     3181 ccagagcgca cgcctgctcg tacgggcagc cgagcgcctg ccatgccgct gccgctgcgc
     3241 gccattcgcc gcgtaactgc agcgcgaacg gcgccgcgca gccgtcggga acctcggcga
     3301 gcgcgccggc gcgccgcaga cagacgccgg tttcgccgag cgcccacgga ttgcgacgca
     3361 gccgtgccag ctcgaagcac gtccgcacct cggccacgca gcgttgcgca tcgccttgca
     3421 gccacgccgc ctcggcacgc gcaaccgcca ccggccagat ccgctgcaat tcgccggaac
     3481 tgcacgcgag cgcgtgcccc tcgtccagcg ccgccgcagc ggccgggtcg ccgcgccgca
     3541 cgcggacgtg tcccagcacc gccagcgccg gaatgcgcgc gacgggcgcg gcgccggagc
     3601 gctcgcacaa ccaggccgct tcgagcgcgg cttcgtccca tcgcccctga tcgaagcgcg
     3661 accgcgtgcg ccacgccatc agatagtgcg accacgaatc caggtcgcgg tccgcgcagt
     3721 aagcaatccc ggcggcaagg tacggctcgg cacgcccgta ctcgcgctgc gagatcgcat
     3781 ggctcgccag gttgcagtag gcgcgcgcgg cgtgctcctc caggccgtgt tcgagcgcga
     3841 gtgcgaggct gcgctcgagc tgcggcgcgc cggccggatc gccgatgcga tcgagactgg
     3901 cgccgacgtt gttcagggcg tgcgccagga tctcggtcgc gtcgagccgt tcggcgaggt
     3961 cgatcgcacg ccggccccac gcgaccgcag cgtcgacatc ttccgccagc atcttcagct
     4021 gcgagaggtt gctgaacgcc atcgccagct cgggcccgtg cggcagcggc tgcagcacct
     4081 gcaccgcggc cgcgccgcag cgctcggcgt cctcgccgtg cccgaggaac cagtgcaggc
     4141 gcgacagcca gcgcaggttc tcgccttccc tcagcgcgtt gccggttgcg cgcgcgagct
     4201 gcagcgccac ctgccgggcc gcgatcgctg cctcgatatc gctgatcagg tagcactcat
     4261 aggcgcgcgc ctcgatcagc gcggcgcgct gcccggcctc caggcgatcc gcgtactgca
     4321 gcgccgccgc gtattgcgcg gccgcctcgc ggtgcgcgcc ccgcgcagcc gcctggcgcg
     4381 cggcaagcgg ggcgaagtgc agcaccgcgt cggcgtcctc ggcgaggttc gcgtggtgcg
     4441 ccagccgcgc cagatccgtc tgggtcgacg gcgcagtccg caatgcgagc agcacggctt
     4501 catgcatcga tacgcgccgc gccggcgcga gcgactccag gatcgcctgg cgcgccagct
     4561 cgtggcggaa tgtgtagcgg ccggcatcga tgcgcagcat cccggaggcc tcgcactggg
     4621 tgattgcatc ggcttcggcg gcggcgaggt gcgcgagcag ccagggctcg acgcgtggcc
     4681 cggccaccgc cgcgtactcg agcaccgcgc gtgccgacgg ggtgagccgc gccgcgcgcg
     4741 ccagcaccgc gtcgcgcacg gtcggcggaa tgccactgcc accggcgccg agcgcctcgg
     4801 tcacgaagaa tgcattgccg ccggtggccg cgtgcagcgc cgcggcgtcg atgctgcggc
     4861 tgccggccag cgtgcgcacc gcatccgcgg tcagcggcgg gatcgtcagc cgctgcaccc
     4921 cggcgctggc gaggtcgccg agcacgacgc gcaacggatg cgacggcccg agctcgtcgt
     4981 cgcggtacgt ggcgatcagc agcgcgcgcg tgcgttcggc acgccgcccg aagtagcgca
     5041 gcaggtcgag cgtcgccgca tcggcccagt gcacgtcttc gaataccgcc accgtcggcg
     5101 cgccgcgcaa cgcgttcagg caggcggcga acagatcgat gcgcgggaca tcgagcggcg
     5161 gcagcgggct gcccgcatcg gcgagctgca gcgcgatgtc ctgcagcgcg ccgagcggcg
     5221 tcggcgtgct catcgcctcg cagttgcccc acagcacccg ggccggttcg cgctgcgatt
     5281 cggcgaagtg cgaaaccagc gctgacttgc cgatgcccgc ctcgccggcg atgaacacca
     5341 tgacgccgcg cccgtcacgc gcgcgctcga aacaccggtt gagtgtctcc aggagggggg
     5401 cgcgttcgag gagctgcatc agtcatcccg atcccggcgc agcggcgcgc tcccggctgg
     5461 cgcgtcgctg cacgcgggaa ttccaaccct gagtttcagg ttcttcttgg agcgcaattg
     5521 tccatccgtc ccgccgcgct tgaagcggtc gtcgcgttac agatgaaatg ccggctcaca
     5581 cggtgccgcg acctgccgcg aactcgcgca gcgcgtgccc cgtgtgcgtg tcgcgccgcg
     5641 ccagttgctg cggcgggcac tgcgcgatca cgcggccgcc gccgtcgcca ccctcggggc
     5701 cgaggtcgac gatccagtcc gcctcggcga tcacgtcgag gttgtgctcg attaccacca
     5761 ccgtgttgcc ggcgcccacc agccggtgca gcaccgcgag cagcttctcg acgtcgtgca
     5821 tcgacagacc cacggtgggc tcgtcgagca cgtacagcgt gtgcaacggt ttgtacgcac
     5881 ggccggtgcg gacgagcccc tcgtccatcc gcaccttggc gagttcggtc acgagcttga
     5941 tccgctgcgc ctcgccgccg gacagcgtcg gcgacggctg gccgagcgtc agatagccga
     6001 gccccacgtc ctgcagcagt tgcagcggcc gcgcgatcga cgggtgcgcg ccgaagaacg
     6061 gcaccgcttc gtcgacttcc atcgccagca cctcgcccgc ggtcttgccg cgcatctgga
     6121 tgccgagcgt ctcgcgattg aagcgccgcc cgccgcacac gtcgcacagc accttcacgt
     6181 ccggcaggaa gttcatctcg atcgtgcgca g
//
LOCUS       DBNHBY010000086         2947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_478376, whole genome shotgun sequence.
ACCESSION   DBNHBY010000086 DBNHBY010000000
VERSION     DBNHBY010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_478376"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..993)
                     /gene="rarD"
                     /locus_tag="AC16HB_02035"
     CDS             complement(67..993)
                     /gene="rarD"
                     /locus_tag="AC16HB_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR5605598.1"
                     /translation="MASEPPTPTPVPAAEAARTGLLAAIGAFTIWGLLPLYLRPLQHV
                     PAMEIVAHRIVWACLLVFAWLAVRGQVGEVRAALADPAARKRLVASALLITINWLLYV
                     WAVTHGHVVDASLGYFINPLLNVVLGVFVLGETLNRAQKLAVAIAAAGVLYLAIVAGR
                     PPWIALVLAASFGLYGLIRKTVQAGAVTGLATETLVLAPFAFAWLVWAQLHGGGAFGD
                     SLPIDALLVGSGLVTALPLALFAFGARRIRLSTVGLVQYIGPTLQFLLGVFLFNEAFT
                     QQRGIGFALIWTALAIYAGDSLWRNRKARALA"
     gene            1156..1530
                     /locus_tag="AC16HB_02040"
     CDS             1156..1530
                     /locus_tag="AC16HB_02040"
                     /inference="COORDINATES: protein motif:HMM:NF023184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HIR5605599.1"
                     /translation="MENSLSAGRLRREWRTMQAMVDIYCRGHEHDRAGAALCKDCSDF
                     LDYAGRRLQKCPYGPAKPTCARCPIHCYKPQPREHAREVMRYAGPRMMLRHPWLSLTH
                     LTDKLRRVSHPMEVRRRNRAAD"
     gene            complement(1532..1723)
                     /locus_tag="AC16HB_02045"
     CDS             complement(1532..1723)
                     /locus_tag="AC16HB_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605600.1"
                     /translation="MKLTWMNGKRSLEISPRARLVDDREAEALLQKLESLQWRPQNGF
                     LKDDWSGRIALDDATEHAA"
     gene            complement(2050..2724)
                     /locus_tag="AC16HB_02050"
     CDS             complement(2050..2724)
                     /locus_tag="AC16HB_02050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR5605601.1"
                     /translation="MNVSEAVSSRISCRAFLPAPVPAATVRAILDAARRAPSGGNLQP
                     WHVHVLTGAPLAELVTAIRRKLPAEPQGEGSEYSIYPAGLVEPYRGRRFQCGEDMYAT
                     INVPRDNKLARLLQFARNYEFFGAPVGLFFSIDRRLGPPQWSDVGMYMQTVMLLAREH
                     GLHTCAQEAWSVWYRTVGEFLELPAELMLFAGMALGYMDPAHPINSLRTQRAPLEEFA
                     TLRGFD"
BASE COUNT          512 a          999 c          992 g          444 t
ORIGIN      
        1 ttcccgggca gtccggaagg cagcgggggt tgctgccaca aatgcagcgc taaacgacgt
       61 tgagcactac gccagcgccc gcgccttccg attgcgccac aggctgtcgc ccgcgtagat
      121 cgcgagcgcc gtccagatca acgcgaaccc gatgccccgc tgctgcgtga aagcctcgtt
      181 gaacaggaac acgccgagca ggaactgcag cgtcggaccg atgtactgca cgagcccgac
      241 tgtcgacagc cggatgcgtc gcgcgccgaa cgcgaacagt gcgagcggca gcgccgtgac
      301 gagcccgctg ccgacgagca gcgcgtcgat gggcaggctg tcgccaaacg cgcctccgcc
      361 gtgcaattgc gcccacacca gccacgcgaa agcgaagggc gccagcacca gcgtttccgt
      421 tgcgagcccg gtcactgcgc ccgcctgcac cgtcttgcgg atcaggccgt acaggccgaa
      481 gctcgcggcc agaaccagcg cgatccacgg cggacgcccc gcgacgatgg cgaggtacag
      541 cacgcccgcc gccgcgatcg cgaccgccag cttctgcgct cggttcagtg tctcgccgag
      601 cacgaacacg ccgagtacga cgttcagcag cgggttgatg aaatagccga ggctcgcgtc
      661 gacgacgtgg ccgtgcgtca cggcccagac gtacaacaac cagttgatcg tgatcagcag
      721 agcggacgcg accagccgct tgcgagcggc gggatcggcg agcgccgctc gcacctcgcc
      781 gacctggccg cgcacggcga gccacgcgaa caccagcagg cacgcccaga caatgcgatg
      841 cgcgacgatt tccatcgccg gtacgtgctg caaggggcgc agatagaggg gcagcaggcc
      901 ccagatcgtg aaagcgccga tcgccgccag cagcccggtg cgcgcagctt cggctgccgg
      961 aacgggggtg ggggtaggcg gttcggaagc catggcggaa gcacgtgcgc cttgcagcgt
     1021 agcggactcg cattctcgca gatcggtcgt cgccgtggcg gtgcccggcc gcgcgtgtca
     1081 gcctgcggcc cgtctcgctg cgcgtcgtgg aaactgcgga gcgtcagcga ccgtccgacc
     1141 cggcaagctg cgcgcatgga gaattcgctg tctgcaggcc gcctgcgccg cgagtggcgc
     1201 accatgcagg ccatggtcga catctactgc cggggtcacg aacacgaccg cgccggcgca
     1261 gcgctgtgta aggattgcag cgattttcta gactatgcag ggcggcggtt gcagaagtgt
     1321 ccctacggcc ccgccaagcc gacctgcgcc aggtgcccga ttcactgtta taaaccgcag
     1381 ccgcgtgaac acgcgcgcga ggtcatgcgc tatgcgggac cgcgcatgat gctgcgtcat
     1441 ccgtggctca gcctgacgca cctcacggac aagttgcggc gcgtgtcgca cccgatggaa
     1501 gtccggcgtc gcaaccgcgc agcggattga ctcaagccgc gtgctcagtc gcgtcgtcga
     1561 gcgcgatacg gccgctccag tcgtccttga gaaacccgtt ctgcgggcgc cactgcaacg
     1621 actcgagttt ctgcagcagg gcctccgcct cacggtcgtc caccaggcgg gcgcgcggcg
     1681 agatctccag gctgcgcttg ccgttcatcc aggtcagttt catcagcgtg ctctccgtgg
     1741 ctgcgatgcg taacaaaggt tcgcatcgct taccctgcat gctgcgtgcc aagcgtggcg
     1801 ggcaagccgg aatgaagcct ggcgcggcga atcgcggtag ttgccagccg tcgtcgcggc
     1861 gcaccgtaaa actgcgcgac gcgcccgatt gaacagaagc gggcgggtcg gacgctggag
     1921 cgtgaagacc gtcacgtaat cgggcagcgt gtccgccatt cgcgacataa cgcggtatcg
     1981 aacatgaact gcgcagtgtc agctttgccg acacggcgaa cagagcctcg gacagcgcgt
     2041 cgccgcgggt cagtcgaacc cgcgcagcgt ggcgaactcc tcgagcggtg cgcgctgcgt
     2101 gcgcagcgag ttgatcgggt gcgccggatc catgtagccg agcgccatgc cggcaaacag
     2161 catcagctct gcgggcagtt cgaggaattc acccacggtg cgataccaga ccgaccacgc
     2221 ctcctgcgcg caggtgtgca ggccgtgctc gcgggcgagc agcatgaccg tctgcatgta
     2281 catgccgacg tcggaccact gtggcgggcc caggcgacga tcgatgctga agaacaggcc
     2341 gaccggcgcg ccgaagaact cgtagttgcg cgcgaactgc agcaggcggg cgagcttgtt
     2401 gtcgcgtggt acgttgatcg tcgcgtacat gtcctcgccg cactggaagc gtcgtccgcg
     2461 gtacggctcc accagtccag ccgggtagat cgaatactcg gagccttcgc cctgcggctc
     2521 ggccggcagt ttgcggcgga tcgcagtcac cagttccgcc agcggcgcgc ccgtgagcac
     2581 gtgaacgtgc catggctgca ggttgccgcc ggacggggcg cggcgcgccg cgtcgaggat
     2641 ggcccgcact gtcgcggcag ggacgggcgc cggcaggaac gcgcggcaac tgatgcggct
     2701 ggagacggct tcgctgacgt tcacttgagt tcctcctcgg cgcgggcgat tctgccagaa
     2761 gcccagcgca ccgcagtcct ctcttgcaca acgtcacgga ccctgtcgcc aagcctgcgt
     2821 ccagcatgag ccgcggctac aagatcaccc tgctcgcggc gctctacgtc gcgcagggcc
     2881 tgccttacgg ctttttcacc caggcgctgc cggtgctgct gcgcgacgcg ggcctttcgc
     2941 tcaaggc
//
LOCUS       DBNHBY010000087         2518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_749403, whole genome shotgun sequence.
ACCESSION   DBNHBY010000087 DBNHBY010000000
VERSION     DBNHBY010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_749403"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..380
                     /locus_tag="AC16HB_02055"
     CDS             15..380
                     /locus_tag="AC16HB_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HIR5605602.1"
                     /translation="MSVELLLATLIGVLAGAGIWLLLRARTFDLMLGLTLLSYAVNMF
                     LFAMGRLRVDAAPIVDPQRAPTLANYADPLPQALVLTAIVISFAMTALILVIALRARA
                     ETGSDHVDGVAPVTTERGE"
     gene            377..1930
                     /locus_tag="AC16HB_02060"
     CDS             377..1930
                     /locus_tag="AC16HB_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HIR5605603.1"
                     /translation="MTAHWLIAPVVLPLFTGAALVVLERRAPARVAPLSIAATAALLL
                     TALVLLARAADGEVEAYLVGNWRAPFGIALALDRLTALMLALTAVVALASVIYARGGD
                     DARGPHFHALFQFQLMGLNGAFLTADLFNLFVFFEVLLIASYGLLLHGGGAARLKAAI
                     HYVGFNLTGSALFLIAVASLYGLTGTLNMADLAQRVAQLPAESAQLARAAALLLLVVF
                     CVKAALLPLYFWLPDTYGAASAPVAALFAIMTKVGVYAVARVYTLVFGADGGVAANVA
                     QPWLPALALATLLLGALGALAATRLRTLVAYLVIASAGTLLLGLGLGRAATVAAALFY
                     LVNSTLVAAVLFLLIDRIGAQRAGDALAPACFGAERSMLGAAFAFCAIAVAGMPPLAG
                     FLGKALLLDAARATPYAAWVWTIVLVSSFGVVVALARAGSTVFWRPALPDAPPSATAP
                     TAPLQASAIGALLIALAAVAFAAQPLAAYTQAAARQLFERDAYISAVFGAVPVPPAFP
                     VRDRLGGKP"
     gene            1927..2409
                     /locus_tag="AC16HB_02065"
     CDS             1927..2409
                     /locus_tag="AC16HB_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR5605604.1"
                     /translation="MKSWLPAPLLSAVLLAVWLLLANTLDPAQFVLGALLALAVPLCV
                     DRGAEQARLRKPLVIARLALIVLWDIVRSNIDVARRILGPEAALNPRFVRVPLDLRDA
                     HGILALAGIITMTPGTLTADVSDDRGHLLVHALNVDDEAALVAQIKARYEQPLREIFE
                     "
BASE COUNT          272 a          891 c          898 g          457 t
ORIGIN      
        1 ttgagttcga gccgatgagc gtcgaactgc tgctcgcgac cctgatcggc gtgctggccg
       61 gcgccggcat ctggctgctg ttgcgcgcgc gcacgttcga cctgatgctc ggcctgacat
      121 tgctgtcgta cgcggtcaac atgttcctct ttgcgatggg ccggctgcgc gtcgacgcgg
      181 cgccgatcgt cgacccgcag cgcgcgccga cgctcgcgaa ctacgccgat ccgctgccgc
      241 aggcgctggt gctgaccgcg atcgtgatca gtttcgcgat gaccgcgctg atcctggtca
      301 tcgcactgcg cgcacgcgcg gagaccggca gcgatcacgt cgacggcgtc gcgccggtga
      361 cgacggagcg cggcgaatga ccgcgcactg gctgatcgcg ccggtcgtgc tgccgctgtt
      421 caccggcgct gcgctggtgg tgctcgagcg ccgcgcgccc gcgcgggtgg cgccgctgtc
      481 gattgcggcg accgcggcgc tgctgctgac cgccctcgtc ctgctcgcgc gcgcggcgga
      541 cggagaagtc gaggcgtacc tcgtcggcaa ctggcgcgcc ccgttcggca tcgcgctcgc
      601 gctcgaccgg ctgaccgcgc tgatgctggc gctgaccgcc gtcgtcgcgc tggcgagcgt
      661 gatctatgcg cggggcggcg acgatgcgcg cggcccgcac ttccatgcgt tgttccagtt
      721 ccagctgatg ggtctgaatg gcgcgttcct gaccgcagac ctgttcaatc tgttcgtgtt
      781 cttcgaagtg ctgctgatcg cctcgtacgg gctgctgctg cacggcggcg gcgctgcgcg
      841 tctgaaggcc gcgatccact acgtcggctt caacctcacc ggctcggcgc tgttcctgat
      901 cgccgtcgct tcgctgtacg ggctgaccgg cacgttgaac atggccgacc tcgcgcagcg
      961 cgtcgcgcag ctgccggccg agagcgcgca gctcgcacgt gcggccgcgc tgctgctgct
     1021 cgtggtgttc tgcgtgaaag cggcgctgct gccgctgtac ttctggctgc ccgacaccta
     1081 cggcgcagcg agcgccccgg tggcggcgtt gttcgcgatc atgaccaagg tcggcgtgta
     1141 cgcggtcgcg cgcgtctaca cgctggtgtt cggcgccgat ggcggcgtgg ccgccaacgt
     1201 cgcgcagccc tggctgccgg cgctcgcgct ggcgactttg ctgctcggtg cgctcggtgc
     1261 gctggctgca acaaggctgc gcacgctggt cgcgtatctc gtgattgcct cggccggcac
     1321 gctgttgctc gggctcgggc tcggacgcgc ggcgacggtg gcagccgcgc tgttctacct
     1381 tgtcaacagc acgctggtcg cggcggtgct gtttctgctg atcgaccgca tcggcgcgca
     1441 gcgcgcgggc gacgcgctcg cgccggcttg cttcggtgcc gagcggtcga tgctcggcgc
     1501 cgcgttcgca ttctgcgcga tcgcggtggc gggcatgccg ccgctggccg gattcctcgg
     1561 caaggcgctg ctgctcgatg ctgcgcgcgc cacgccgtac gcggcgtggg tgtggaccat
     1621 cgtgctcgtg tcgagcttcg gcgtggtcgt cgcactggca cgcgcgggca gtacggtgtt
     1681 ctggcgcccc gcgctgcccg acgcgccgcc gtccgcaacg gcgccgaccg caccgctgca
     1741 ggcgagcgcg attggcgcct tgttgatcgc gcttgccgcg gtggcgttcg ccgcgcagcc
     1801 gctggccgcg tacacgcagg ctgccgcgcg gcagttgttc gagcgcgacg cctacatcag
     1861 cgcggtgttc ggcgcggtgc cggtgccacc ggcatttccg gtgcgcgatc ggctcggggg
     1921 caagccgtga aatcgtggct gcccgcgccg ctgctgtccg ccgtgctgct cgcggtgtgg
     1981 ctgctgcttg ccaacacgct cgatcctgcg cagttcgtgc tcggcgcgct gctggcgctc
     2041 gcggtgccgc tctgcgtcga tcgcggcgcc gagcaggcgc gcctcaggaa gccgctcgtg
     2101 atcgcgcggc tggcgctcat cgtgctgtgg gacatcgtgc gctccaacat cgacgtggcg
     2161 cgccgcatcc tcggcccgga agcggcgctc aacccgcgct tcgtccgggt gccgctcgat
     2221 ctgcgcgacg cgcacggcat cctcgccctc gccggcatca tcacgatgac gccgggcacg
     2281 ctgacggccg acgtgagcga cgaccgcggc catctgctgg tgcatgcgct gaacgtcgac
     2341 gacgaagccg cgctcgtcgc ccagatcaag gcgcgctacg aacaaccgct gcgggagatc
     2401 ttcgaatgat gcttgccgta ctgccgttcg ccatcggcgc gttcgtggtt gccttgctgc
     2461 tcgccgcatg gcggctcatg gccgggccga gcctgcccga ccgcatcctc gcgctcga
//
LOCUS       DBNHBY010000088         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1358408, whole genome shotgun sequence.
ACCESSION   DBNHBY010000088 DBNHBY010000000
VERSION     DBNHBY010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1358408"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16HB_02070"
     CDS             <1..283
                     /locus_tag="AC16HB_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5605605.1"
                     /translation="WLKQQNAAPTTALLDADGTVGRAYGARTTPHMFVIDPAGKVRYA
                     GAIDDRRSTNPADVRTSNKYVAQALGELRSGKPVTVASTPPYGCSVKYE"
     gene            complement(296..>1587)
                     /gene="dacB"
                     /locus_tag="AC16HB_02075"
     CDS             complement(296..>1587)
                     /gene="dacB"
                     /locus_tag="AC16HB_02075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR5605606.1"
                     /translation="PPTASAIVVLPLSGSGPSIAVNDAVPMNPASTMKLVTTYTALGM
                     LGPAYTWRTEAFATGALRRDLLDGDLVIRGSGDPRLVIEHLWLLVNRIRGSGVREIRG
                     NVVLDKSAFEPLVHDPALFDGEVNRPYNAGPDALLLNFKALTLGFVPDPDARTVRVVA
                     TPALAGLKLQESIRGIDGHCGDWRARLQPDFADPMAPVFRGAYPMACGEKTWAISVLG
                     HTQYFGAAFRALWESAGGVWKASVREGAVPPDARRIALHESAALAEVIRDINKFSNNV
                     MTRQLYLTMGAVASGQPGNPERAGRAVRSWLASKGLDMPELVLENGAGLSRIERISAG
                     NMARLLAHAYAAPVMPEFMSSLPLAGVDGTMKRRNGAAASAHVKSGMLADVRAIAGYV
                     LAASGRRYAVVAIINHPNARDAQGVHDALLQWVYQNG"
BASE COUNT          256 a          554 c          539 g          238 t
ORIGIN      
        1 gtggctgaag cagcagaatg cggcgccgac caccgcactg ctcgacgccg acggcacggt
       61 gggccgtgcc tacggcgcac gcacaacgcc acacatgttc gtgatcgacc cggcgggcaa
      121 ggtgcgatac gcgggcgcga tcgacgacag gcgcagcacg aacccggccg acgtgaggac
      181 ctcgaacaaa tacgtcgcgc aggcgctcgg cgaattgcgc agcggcaagc cggtcacggt
      241 ggcatcgacg ccgccgtacg gctgctcggt caagtacgag tagcggcgcc cggcatcagc
      301 cgttctgata cacccactgc aggagtgcgt cgtgcacgcc ctgcgcgtcg cgcgcgttcg
      361 gatggttgat gatcgcaacc accgcgtagc ggcggccgct cgcggcgaga acatagcccg
      421 cgatcgcgcg cacgtcggcc agcatgccgg acttgacgtg cgcgctggcg gcggcgccgt
      481 tgcgccgctt catcgtgccg tcgacgcccg ccagcggcaa cgacgacatg aactccggca
      541 tgacgggcgc ggcatacgcg tgcgccagca ggcgcgccat atttccggcc gagatccgtt
      601 cgatgcgcga caggccggcg ccgttttcca gcacgagttc cggcatatcg aggcccttgc
      661 tcgcaagcca gctgcgcacc gcgcgccccg cgcgctcggg gttgcccggc tgcccgcttg
      721 cgacggcgcc catcgtcagg tagagctgcc gcgtcatcac attgttgctg aacttgttga
      781 tgtcgcggat tacttcggcc agcgcggcgg actcgtgtag cgcgatgcgc cgcgcgtccg
      841 gcggcaccgc gccttcgcgc acgcttgcct tccagacacc tccggcgctt tcccacagcg
      901 cgcgaaaggc cgcgccgaag tattgcgtgt gtcccagcac gctgatggcc caggttttct
      961 cgccgcacgc catagggtag gcaccgcgaa acacgggcgc catcgggtcg gcgaaatccg
     1021 gctgcagccg tgcgcgccag tcgccgcagt ggccatcgat gccgcggatc gattcctgca
     1081 gcttcagtcc cgccagcgcc ggtgtggcga cgacgcgcac ggtacgcgca tccggatcgg
     1141 gtacgaagcc cagcgtcagg gccttgaagt tcaacagcag cgcatcggga ccggcgttgt
     1201 aggggcggtt cacctcgccg tcgaacagcg cgggatcgtg caccagcggt tcgaaggccg
     1261 acttgtcgag cacgacgttg ccgcgaatct cgcgcacgcc agagccgcgg atgcggttca
     1321 ccagcagcca caggtgctcg atcaccaggc ggggatcgcc gcttccgcgt atcacgaggt
     1381 cgccgtccag caggtcccgc cgcagcgcgc cggtggcgaa cgcttctgtc cgccacgtgt
     1441 acgcgggccc gagcatgccc agcgcggtat aggtcgtcac gagcttcatc gtcgatgccg
     1501 gattcattgg cacggcgtcg ttgacggcga tcgacgggcc gcttcccgag agcggcagca
     1561 cgacgatcgc ggacgccgtc ggcggga
//
LOCUS       DBNHBY010000089         5756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1425924, whole genome shotgun sequence.
ACCESSION   DBNHBY010000089 DBNHBY010000000
VERSION     DBNHBY010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1425924"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="AC16HB_02080"
     CDS             <1..155
                     /locus_tag="AC16HB_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR5605607.1"
                     /translation="ALLGGDGDVVADIFRSRVLVRGKGSEVWGWGTAYGKATGGSTPP
                     AKATKS"
     gene            152..382
                     /locus_tag="AC16HB_02085"
     CDS             152..382
                     /locus_tag="AC16HB_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HIR5605608.1"
                     /translation="MKRILIVAGLVVALAACGERPQSGSTARKIDTKAWEGAQNAFVA
                     PGWKPGDQASWESQMRSRAMGQNEYLRTGPRQ"
     gene            407..1546
                     /locus_tag="AC16HB_02090"
     CDS             407..1546
                     /locus_tag="AC16HB_02090"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR5605609.1"
                     /translation="MQRLMRQFAVGSALALGLLASAPAQQAVASPDSAKGPPAGFVAP
                     PEPKPDETNAVRDKTQPGNNAPFWRAVRKSGEQQGFVTLPGAEQGTLIQRFVQYPGSR
                     YTTAGEAWREVRNRWIIPYGGALTLLVIVAIALFHWRKGPIGGRHPDSGRRIERFTPF
                     ERAAHWVNAIAFVILGISGLVMAFGKFFLLPVMGLTLFGALTYGLKTLHNLAGPLFAL
                     SLIVIFVTFVRDEMPRRGDLEWLRKGGGMLSGAEVPSHRFNAGEKVLFWIGMVVLGVI
                     VVASGLYLDKVLPGLDYLRGDMQIAHMVHAVSTILMTVLLLGHIYLGSIGVKGAYQGM
                     RTGYVDESWAHEHHALWHEDIKAGRIPAQRSGAAAPAARPAGAHS"
     gene            1574..1897
                     /locus_tag="AC16HB_02095"
     CDS             1574..1897
                     /locus_tag="AC16HB_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605610.1"
                     /translation="MNRITVSLTMLATLLCAGALSAKLPPPSDEAKSKAAEAAAKNAY
                     ADKVAAYKLCKSMDKVAAEYVAAQKKAGKAVKPPTPTPPCADPGAFVSTPATPAVAAA
                     APAKK"
     gene            1904..2815
                     /locus_tag="AC16HB_02100"
     CDS             1904..2815
                     /locus_tag="AC16HB_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5605611.1"
                     /translation="MSPPGEALQTVAPAVEWPTVPDAPRLTEARGELLREIVILDEYG
                     ERRTILIPVERPLTVFVDKRELVTLMTLGAMPELLVLGYLRNQRLIERVEQVESITVD
                     WEVEAAAVRSRDGLHDLEARTARRVVTTGCGQGTVYGDMMSQIERIKLPGPAVARIRQ
                     STLRRMLESIRQQDNIHRRAGSVHGCALFRGAEMLMFVEDVGRHNAIDTIAGWMAMHG
                     VEGADKVFFTTGRLTSEMVMKSAQIGVPIIVSRNGVTAMGFELASRLGITLFGRAANR
                     HFLCYTGAERFDSEPEPQRAPVRVVSG"
     gene            complement(2829..4103)
                     /gene="moeA"
                     /locus_tag="AC16HB_02105"
     CDS             complement(2829..4103)
                     /gene="moeA"
                     /locus_tag="AC16HB_02105"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5605612.1"
                     /translation="MSEPGPSPLAAGESNLSVAQARAAIAAALQPIRDVQEVSLLDAL
                     GRVLAVDVVSPIDVPAHDNSAMDGYAFASSALRAEGPTVLRAVGTIHAGAAFDGMVGA
                     GECVRIMTGAVMPAGLDTVVPLELCQIEGDRVRVEPGVIQPGENRRCRGEDLARGRPA
                     LHAGRVLKPADLGLAASLGIEHVTVMRRLRVALFSTGNELRTLGQTLDAGCIYDSNRY
                     SLRGALQRIGMDTIDLGVVRDDPQALQRTLASALAQADVVLTSGGVSAGDADYTRDVL
                     DRMGKVAFWKVAMRPGRPFAFGPIRGQESDRTIWLFALPGNPVAALVTFYVFAREALL
                     QLAGARPEPLPVLQARSTRAIRKRPGRTEFQRGRVERAQDGSWQVQITGSQGAGILRS
                     MSEANCLVVLNHEQGSVAAGEPVDVWLFDGLT"
     gene            4061..4984
                     /locus_tag="AC16HB_02110"
     CDS             4061..4984
                     /locus_tag="AC16HB_02110"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605613.1"
                     /translation="MIRPRREATAQAPTSCDSVRDSFDDRSIIPRGAPDSRGADADNE
                     SMQSASYRQPGWPNLDSFPFPARSGPVNPFSDSLVLALRLISNADANLLGIVGLSLRV
                     STTACAIGAIIGISLGMWLALARVRLRHGVVWFVNTLLALPSVVVGLIVYLLLSRSGP
                     LGSFGILFTPAAMIAAQSILVVPLFAALSRRLLLDGLREGGDQLRSLGATPLQSAVLM
                     LVHERLGLLTIVLTGFGRAVSEVGAVMIVGGNIEGFTRVMTTAIALETSKGDLPLALA
                     LGIVLLGLVGCANLLIGLMQGRSPIVREAFA"
     gene            4992..5744
                     /locus_tag="AC16HB_02115"
     CDS             4992..5744
                     /locus_tag="AC16HB_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5605614.1"
                     /translation="MDRARIVTQSPPAIPLVRLERATVQFGDVTALRDVSLTLHRGER
                     LALVGANGSGKTTLLRLLHGLLRDAKGARAIFPLHPEGRPPVAAMMFQRPFLLRVSAR
                     WKVLPALWLHGVRDRAERERRCHRALQRVGLEHVANRLATSLSGGQQQRLALARAWAV
                     EPDILLLDEPTANLDPSGKREVEALIEEIAHEGVTVVISTHNLGQTKRLATRVAYLEA
                     GRIVVDLPVERFFTDRLPHEAEQFLKGELPWG"
BASE COUNT          898 a         1933 c         1961 g          964 t
ORIGIN      
        1 aggcactgct cggcggcgac ggcgacgtgg tcgccgacat cttccgcagc cgcgtgcttg
       61 tccgcggcaa ggggagcgaa gtgtggggct ggggcactgc ttacggcaaa gcgacgggcg
      121 gttcgacccc gccggcgaag gcgaccaaat cgtgaaacgg atcctgatcg tggctggact
      181 ggtggtggcg ctggccgcct gcggcgaaag acctcagagc ggatccaccg cgcgcaagat
      241 cgacaccaag gcatgggagg gcgcgcagaa cgcgttcgtt gcgccgggat ggaagcccgg
      301 cgaccaggcg agttgggaat cgcagatgcg cagcagggcg atggggcaga acgaatatct
      361 gcgcaccggg ccccgccagt agaacgcgtc gaacgaagga ggcctcatgc aacgcttgat
      421 gcgccagttt gcggtgggca gcgcgctcgc gcttgggttg ctcgcgtccg cgccggccca
      481 gcaggcggtt gcgagcccgg attccgccaa gggtccgccc gccgggttcg tggcgccgcc
      541 cgagccgaag ccggacgaga ccaacgcagt gcgggacaag acccagcccg gcaataacgc
      601 gccgttctgg cgtgccgtgc gtaaatcggg cgagcagcag gggttcgtga cgctgccagg
      661 cgcggagcag ggaacgctga tccagcgctt cgtgcagtac ccgggttcgc gctatacgac
      721 ggccggcgaa gcgtggcgcg aagtgcgcaa ccgctggatc atcccctatg gcggtgcatt
      781 gaccctgctc gtcatcgtcg cgatcgcact gttccactgg cgcaagggcc cgatcggcgg
      841 ccggcatccc gacagtggcc gcaggatcga acgcttcacg ccgttcgagc gcgcggccca
      901 ctgggtcaat gcgatcgctt tcgtcatcct tggcatctcc gggctcgtga tggccttcgg
      961 caagttcttc ctgctgccgg tcatgggact cacgctgttc ggcgcgctga cctacgggct
     1021 caagaccctg cacaacctcg cgggaccgct gtttgcgcta tcgctgatcg tcatctttgt
     1081 tacgttcgtg cgcgacgaaa tgccgcgccg aggcgatctc gagtggctgc gcaagggtgg
     1141 cggcatgctc agcggcgccg aggttccgtc gcaccgattc aatgctggcg aaaaggtgct
     1201 gttctggatc ggaatggtcg tgctcggcgt catcgtcgtg gcgtccggcc tgtacctcga
     1261 caaggtcctg ccggggctcg actacctgcg cggcgacatg cagatcgccc acatggtgca
     1321 tgccgtgtcg acaattctga tgacggttct gctgctcggg catatctacc tcggcagcat
     1381 cggcgtgaag ggagcctacc aggggatgcg caccggctat gtcgacgagt cgtgggcgca
     1441 cgagcatcac gcgctgtggc atgaggacat caaggcgggc cggattcccg cgcaacgctc
     1501 gggggcggca gcgcccgcgg cgcgcccggc cggcgcccat tcctgattcg atcgcgcaac
     1561 ccggagccat cgcatgaacc gaatcaccgt ctccctcacg atgctggcca cgttgctgtg
     1621 cgcgggagcc ctgtcggcca agctgccgcc gccgagcgac gaagccaagt ccaaggcggc
     1681 ggaagcagcc gcgaagaacg cctacgccga caaggtggcc gcctacaagc tctgcaagag
     1741 catggacaag gtcgcggcgg agtacgtcgc cgcgcagaag aaagccggta aggccgtcaa
     1801 gccgccgaca ccgacgccgc cttgcgccga tccgggcgcc ttcgtctcta cgccggccac
     1861 gcccgcggtt gccgctgcgg caccggccaa gaagtagccg cgcatgtccc cgcccggaga
     1921 agccctgcaa accgtcgcgc cggcggtgga atggccgacc gtgccggatg cgccgcgtct
     1981 gacggaggca cgcggcgaac tgctgcggga gatcgtcatc ctcgacgagt acggcgagcg
     2041 gcgcaccatc ctgatcccgg tcgagcgccc gttgaccgtg ttcgtcgaca agcgtgaact
     2101 cgtcaccttg atgacgctgg gcgcgatgcc ggaattgctg gtgctcggct atctgcgcaa
     2161 tcagcgtttg atcgaacgtg tcgagcaggt ggagtcgatc actgtcgact gggaggtcga
     2221 ggcagccgct gtgcgatcgc gcgatggcct gcacgacctc gaggcgcgca cggcgcggcg
     2281 agtggtgacg accggctgcg gacagggcac cgtctacggc gacatgatgt cgcagatcga
     2341 gcgcatcaag ctgcccggcc ccgcggtcgc gcgcattcgg cagagcacgc tgcggcgcat
     2401 gctcgagtcc atccggcagc aggacaacat tcatcgccgg gccggatccg tacatggctg
     2461 cgcgctgttc cgcggcgccg agatgctgat gttcgtcgag gacgtggggc gccacaacgc
     2521 gatcgacacc atcgcgggct ggatggcgat gcacggcgtg gaaggggccg acaaggtctt
     2581 cttcaccacc ggacggctga ccagtgagat ggtgatgaag tcggcgcaga tcggcgtgcc
     2641 catcatcgtg tcgcgcaacg gcgtgaccgc gatgggcttc gaactcgcga gccggctcgg
     2701 catcacgctt ttcgggcgcg ctgcgaaccg gcacttcctg tgctacacgg gcgcggagcg
     2761 gttcgactca gaacccgagc cgcagcgcgc cccggtgcgc gtggtgtcgg gctgaagcgc
     2821 gggcggcttc aggtcaatcc gtcgaacagc cagacgtcga ccggttcgcc ggcggcgacc
     2881 gacccctgtt cgtggttcaa gacgaccagg cagttggcct cgctcatgct gcgaaggatt
     2941 ccggccccct gcgacccggt gatctgtacc tgccagcttc cgtcctgcgc cctttcgacg
     3001 cgaccgcgct ggaattcggt ccgccctggc cgtttccgaa tcgcccgcgt gctgcgcgcc
     3061 tgcaggactg gcagcggctc cggccgtgcg ccggcgagtt gcaggagcgc ctcgcgcgcg
     3121 aacacgtaaa acgtgaccag tgcggcgacc gggttgccgg gcaacgcgaa caaccagatc
     3181 gtccggtccg actcctgccc gcgtatcggg ccgaacgcaa acggccgtcc cggacgcatt
     3241 gcgaccttcc agaacgcgac tttgcccatg cgatccagca cgtcgcgcgt gtaatccgcg
     3301 tcgcccgcac tgacgccgcc gctcgtaagc accacgtcgg cctgcgcgag ggccgacgcc
     3361 agcgttcgct gcagcgcttg cggatcatcg cgtaccacgc cgaggtcgat ggtgtccatg
     3421 ccgatgcgtt gcagcgcgcc gcgcaggctg tagcggttgc tgtcgtatat gcagcccgca
     3481 tcgagcgtct gaccgagcgt tcgcagttcg ttccccgtgg aaaacagtgc cacgcgtagc
     3541 cgccgcatca ccgtcacgtg ctcgatcccg agcgaagcgg ccagtccgag atcggccggc
     3601 ttcaatacgc gaccggcatg tagcgctggc ctgccgcgtg ccagatcttc gccgcggcaa
     3661 cgtcggttct cgccgggctg gatcacgccg ggctcgaccc ggacccggtc gccttcgatc
     3721 tgacacagtt ccagcggcac cacggtgtcg agccccgccg gcatcaccgc cccggtcatg
     3781 atgcgcacgc attcgccggc gccgaccatg ccgtcgaatg ccgcgccggc atgaatcgtt
     3841 ccgaccgcgc gcagcacggt tggcccctcc gcacgtagcg cgctcgatgc gaacgcgtag
     3901 ccgtccatcg ccgaattgtc gtgtgccgga acgtcgatcg gcgacacgac gtcgacggcc
     3961 agcacgcggc cgagcgcatc gagcagcgac acctcctgaa catcgcggat cggctgcagc
     4021 gcggcggcga tggcggcgcg cgcttgagca acgctgaggt ttgattcgcc cgcggcgaga
     4081 ggcgacggcc caggctccga catcttgcga ctccgtacgg gactcgttcg acgaccgctc
     4141 gattatcccc cgcggcgcgc ccgattcccg cggtgcggac gcggacaacg aatctatgca
     4201 gtccgcttca tatcgccagc ccgggtggcc caacctagac tcgttcccct ttcctgcgcg
     4261 gtccggtccc gtgaatcctt tctctgacag cctggtgctc gccctgcgtc tgatctcgaa
     4321 tgcagacgcg aatctgcttg gcatcgtcgg tctgtctctg cgcgtgagta cgaccgcctg
     4381 cgcgatcggc gcgatcatcg gcatatcgct cggcatgtgg ctcgccctgg cccgggtgcg
     4441 gctgcggcac ggcgtggtgt ggttcgtgaa caccctgctg gcgctgccct cggtggtggt
     4501 cggcctgatc gtctatctgc tgctgtcgcg cagcgggccg ctcggatcgt tcggcatcct
     4561 gtttacaccg gcggccatga ttgcggcgca gagcatcctc gtggttcccc tgttcgccgc
     4621 gctgtcacgc cggcttctgc tcgacggtct gcgcgaagga ggcgaccagc tgcgctcgct
     4681 cggcgcgact ccgctgcaga gtgcggtgct gatgctggtt catgagcggc tcgggctcct
     4741 gaccatcgtt ctgaccggct tcggacgcgc ggtatcggag gtcggcgcgg tgatgatcgt
     4801 cggcggcaac atcgagggtt tcacgcgtgt gatgacgacc gccatcgcgc tggagacgag
     4861 caagggcgat ctgccgctcg cgctcgcgct cgggatcgtg ctgctcggct tggtcggctg
     4921 cgccaatctg ctgatcggcc tgatgcaggg acgcagtccc atcgtgcgcg aggcgttcgc
     4981 ctgaaccttg cgtggatcgc gcgagaatcg tgacgcagag cccgcccgcc atccctctgg
     5041 tgcgcctgga gcgcgcgacg gttcaattcg gcgacgtgac ggcactgcgc gatgtcagcc
     5101 tgacgctgca tcgaggcgag cggctcgcgc tcgtcggtgc caatggctcg ggcaagacca
     5161 cgctgctgcg actgctgcat ggcctgctgc gcgacgcaaa gggcgcgcgt gcaatctttc
     5221 cgctgcaccc tgaggggcgg ccgccggtgg cggcgatgat gttccagcgc cccttcctgc
     5281 tgcgcgtgtc ggcgcgctgg aaagtgttgc cggcgctctg gctgcacggc gtgcgggaca
     5341 gggccgagcg cgagaggcgt tgccatcggg ctctgcagcg cgtcggcctc gaacatgtcg
     5401 ccaatcgcct cgcgacttcg ctctcgggcg gccagcagca gcgccttgcg ctcgcccgcg
     5461 cgtgggcggt cgaaccggac atcctgcttc tcgatgagcc gaccgcgaat ctggatccca
     5521 gcggcaagcg cgaggtcgag gcgttgatcg aagagatcgc gcacgagggc gtgaccgtgg
     5581 tgatcagcac gcacaacctc ggtcagacca agcgcttggc cacccgcgtg gcctacctcg
     5641 aggccggccg catcgttgtc gatctgccgg tcgagcgttt tttcaccgac cgcctcccgc
     5701 acgaggccga gcagttcctg aaaggagaac tgccatgggg ctgatcgacc gactgc
//
LOCUS       DBNHBY010000090         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_884843, whole genome shotgun sequence.
ACCESSION   DBNHBY010000090 DBNHBY010000000
VERSION     DBNHBY010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_884843"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1063)
                     /locus_tag="AC16HB_02120"
     CDS             complement(59..1063)
                     /locus_tag="AC16HB_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605615.1"
                     /translation="MDYVVEGSVRSDRKRVVVHAALATAPAGEQLWSERIEVERAAAS
                     DVREQIAARVARSLRLALINAQAETARHRMHPDTQDLIWQAWSLWERYDPRSNREARA
                     IFERAVERDPKSALAWAGLATTWISEALGHRDVSTDIAVALEHAEAAARVAYDLDRND
                     PHAIQIWGAVLSTRGRIEEGQALIERRLEMEPNNPLSLLWTALNRLHLGQPERALLAI
                     DEALAVSPRDGLVHIFHLQRAQALFDLQRFDESVLSARRSLAARDDSMHARLMLAAAL
                     QRSGNAAAAQLALAEAAASRPGLTVERVAQESMAVNAEGKAMRARYFDALRDAGLPAR
                     "
     gene            complement(1331..1636)
                     /locus_tag="AC16HB_02125"
                     /pseudo
     CDS             complement(1331..1636)
                     /locus_tag="AC16HB_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1798..2532
                     /locus_tag="AC16HB_02130"
     CDS             1798..2532
                     /locus_tag="AC16HB_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5605616.1"
                     /translation="MGDCAGFVAPVVPIELTSSGTLRAGNFRGPLFVRRNNRGELSGF
                     SIDLMQELARRAGRPLEVEYFDAPTALLEALRVGRSDLGLLTDLPARHAFCEFTPPFM
                     ETEETIAVHPSLALRHVDEVDRPGLRIAVVRGTSTDAALTLRLRHAELVRVNNRDEYL
                     AAHTQGVQAYANDRNNLEGQLHKLPGWKILDGAFNTVPMSVALPKGRTAALGWVSATL
                     ESLKREGFFEAAFARMPLQASVVRRP"
     gene            complement(2545..>3150)
                     /locus_tag="AC16HB_02135"
     CDS             complement(2545..>3150)
                     /locus_tag="AC16HB_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5605617.1"
                     /translation="KALAPDQAVQLAAHATDGSALQLRDRALVELLYSSGLRLSELVS
                     LDWRHFGATGNQSASTSWIDLEARQVTVLGKGRKVRAVPIGSAALDALRAWLPVRARI
                     RNVDERALFVSERGGRIAPRSVQQRLDLLARRVGLGVHVHPHVLRHSMASHVLQSSGD
                     LRAVQELLGHANISTTQIYTRLDWQHLAKAYDAAHPRAKRR"
BASE COUNT          514 a         1088 c         1015 g          533 t
ORIGIN      
        1 ccgcaacgac cgctccttgc cgacgcgcag cgtgaccgtc ttcatgggtt cgctgcgatc
       61 agcgtgcggg caacccggcg tctcgcaggg cgtcaaagta acgcgcccgc atcgccttgc
      121 cctcggcgtt cactgccatc gactcctgtg ccacgcgttc tactgtcaga cccggccgcg
      181 atgcggccgc ttcggccaac gccaactgcg ctgcggccgc attgccgcta cgctgcaacg
      241 ccgccgccag catcaggcgc gcatgcatag agtcatcgcg cgcagcgagc gatcggcgcg
      301 ccgacagcac ggattcatcg aaccgctgca agtcaaacag cgcctgggcg cgctggaggt
      361 gaaaaatgtg gacaagtcca tcgcgcggac tgaccgccaa cgcttcatcg atcgcgagca
      421 gcgcgcgctc cggctgtccg agatggagac gattcagcgc ggtccagagc agcgaaagcg
      481 gattgttcgg ctccatttcg agccggcgtt cgatcaacgc ctggccttcc tcaattcggc
      541 cgcgcgtcga cagcaccgcg ccccagatct gaattgcatg gggatcattg cggtcgagat
      601 cgtaggcgac acgtgcggcg gcctcggcat gctcgagcgc aacggcaata tcggtgctga
      661 cgtcgcgatg gccaagcgcc tcggagatcc aggttgttgc caggcccgcc cacgccagcg
      721 ccgatttcgg gtccctctcg acggcacgtt cgaagatcgc acgcgcctcg cggttgcttc
      781 gcgggtcgta gcgctcccac aacgaccacg cctgccagat cagatcctgc gtgtccgggt
      841 gcatgcgatg ccttgccgtc tcggcctgcg cattgatcag cgccagtcgc agactgcggg
      901 caacacgcgc tgcgatttgc tcgcggacat ccgacgcggc ggcgcgttcg acttcgatcc
      961 gctcggacca gagttgctcc ccggccggtg cggttgccag cgccgcatgc acgacgacgc
     1021 gcttgcgatc gctgcgcacc gatccctcga cgacgtagtc cactccgagt tcttcatgca
     1081 gtcggcgcgg gtcggccgct gcatcgcgat agcgcagcgc gcttcctcgc gcaatcacgc
     1141 gcgttccggc aatgttgcca agccgcgtcg tgatctcttc tgtcagaccg tcggcgagcg
     1201 gctgctgggt tccttcgccg atcttgcgca gcggaagcgc cacgatcacg gggcgcgacg
     1261 cggcggtcgg aggcgcgatc gccgtcggtg cagcccgtgc cgcggtcacc tcgccggcaa
     1321 actgataacc gatgcgcggc accgtacgca ccaggcgctc gcgcaccggg ccgagtgcgc
     1381 ggcggatctc cttgatgcac tgggtcagcg aatcctcggt caccaccgcg tcgttccaga
     1441 ccgcgcgcag caactcgtcc ttgctgacca ggcgaccgcg ctggatgaga aggtattcga
     1501 gcactgcggc agtacggggc cgcaacggca cggccgaccc gtcgcagatc agctcgccgc
     1561 ttcgcgggtg aaacgtcgtg ccggcgaacg acagcggttc cgccgccgct acacgttccg
     1621 atgactgcgt gaacacgcgc gctcgttctc tcggatcatt gaccttaccg aaagtttacc
     1681 gacggcttca ggacacgcag cccccgtgtg cgagatgctg acggctctgg tgaagactcg
     1741 agaagcacca tgccgactcg tcgttccatt ctcgtcgcgc tcgctgcccc gctgggcttg
     1801 ggcgactgcg cgggcttcgt tgcacccgtc gtgccgatag agctgacctc cagcggcact
     1861 ctccgggccg ggaactttcg cggcccattg ttcgtgcgcc gcaacaaccg cggtgaattg
     1921 agcggattct ccatcgacct gatgcaggaa ctggcacggc gcgccggccg gccgcttgag
     1981 gtcgagtact tcgacgctcc aacggcgctc ctggaagcac tgcgcgtcgg acgcagcgac
     2041 ctcggcctgc tcaccgatct gccagcgcgg cacgcgttct gcgaattcac gccgccattc
     2101 atggagaccg aagagacgat cgcggtgcac ccctcgctgg cgttgcggca cgtcgatgaa
     2161 gtcgaccggc cgggactgcg cattgccgtg gttcgcggca cgtcgaccga cgcagcgctg
     2221 actttgcgat tgaggcacgc ggaactggtg cgggtgaaca accgcgacga gtatctggcg
     2281 gcgcacacgc aaggcgtcca agcgtacgcg aatgatcgca acaatcttga gggccagttg
     2341 cataagctgc ctgggtggaa gattctcgac ggcgccttca ataccgtgcc catgtcggtc
     2401 gcgctgccca agggacgaac cgcggcgttg ggctgggtaa gcgccacgtt ggagtcgctg
     2461 aagcgcgaag gcttcttcga agcagccttc gcccgcatgc ctctgcaggc cagcgtcgtg
     2521 cgcagaccct gaagcgctat ccggtcagcg gcgcttcgcc cgcggatgcg ccgcgtcgta
     2581 ggccttggcc aggtgctgcc agtcgagccg cgtgtagatc tgtgtcgtcg agatgttcgc
     2641 gtggccgagc aattcctgca ccgcgcgcag gtcgccgctc gattgcagca catgcgaggc
     2701 catcgagtgc cgcagcacgt gcggatgcac gtgcacgccg agcccgacgc gccgcgccag
     2761 caggtcgagt cgctgctgca cgctgcgcgg cgcgatgcgg ccgccgcgct cagagacgaa
     2821 cagtgcccgt tcgtcgacat tgcgaattcg cgctcgcacc ggcagccacg cgcgcagcgc
     2881 gtccagcgcg gccgacccga tcggtactgc gcgcaccttt cggcccttgc ccagcacggt
     2941 gacctggcgc gcttcgagat cgatccaact ggtcgatgcc gattgattgc cggtcgcgcc
     3001 gaaatgccgc cagtcgaggc ttacgagttc ggatagccgc agaccgctcg aatacagcag
     3061 ttcgaccagc gcgcggtcgc gcaactgcag cgccgagccg tcggtcgcgt gcgcggcaag
     3121 ctggaccgcc tgatccggcg caagcgcctt
//
LOCUS       DBNHBY010000091        17718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1494155, whole genome shotgun sequence.
ACCESSION   DBNHBY010000091 DBNHBY010000000
VERSION     DBNHBY010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1494155"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..663
                     /gene="phaR"
                     /locus_tag="AC16HB_02140"
     CDS             124..663
                     /gene="phaR"
                     /locus_tag="AC16HB_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR5605618.1"
                     /translation="MPTAQRLIKKYPNRRLYDTQTSTYITLADVKELVLEQTDFQVVD
                     AKTGEDLTRQILLQIILEEEQEGQPMFTPPVLSQIIRSYGNAMQGMMGSYLEKNVQAF
                     IDIQNKLQEQSKAFYDSNKIGPEMWTQFVNMQAPMIQSMMSNYIEQSKNLFVQMQENM
                     QNQARSMFNTFPFQGGDKK"
     gene            683..2053
                     /gene="rimO"
                     /locus_tag="AC16HB_02145"
     CDS             683..2053
                     /gene="rimO"
                     /locus_tag="AC16HB_02145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR5605619.1"
                     /translation="MTVSSLARRARKPVAPRGVPTVGFVSLGCPKALVDSERIVTQLR
                     AEGYALSSTYDSADLVIVNTCGFIDAAVQESLDAIGEALHENGRVIVTGCLGAKTDGG
                     ANLVAKIHPKVLAVTGPHATDEVMGHVHHHLPKPHDPFTDLVPPAGIKLTPRHYAYLK
                     IAEGCNHHCTFCIIPSMRGPLVSRPIGEVMHEAEGLVQQGVKEILVVSQDTSAYGVDV
                     KYKLDFVGGRAVKTRMTMLAQELGRLGVWVRLHYVYPYPHVDEVIPLMATGKVLPYLD
                     VPFQHAHPAVLKRMKRPADGERNIERIRAWRAICPDLTIRSTFIAGFPGETKDQFEYL
                     LDFLREARLDRVGCFAYSPVAGAAANGLDGALPDEVRDERRARFMQVQEAISRERLRR
                     KVGSVQRVLVDEVMAEGARARSTADAPEIDGVVHVKTSRKLKVGEFFDVRIDASDAHD
                     LSGRLV"
     gene            complement(2050..2883)
                     /locus_tag="AC16HB_02150"
     CDS             complement(2050..2883)
                     /locus_tag="AC16HB_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5605620.1"
                     /translation="MFRYPPAVLALGAIALWMSVAALAVSLKSVPPFLLVGAPMTIVG
                     LVTLPTWRAWRVPPATLAVGVYGLFGFHLLLFTALRLAPPVEANLINYLWPLLIVVLA
                     PLLLPGERLAPRHVIGALMGFSGAALVITGGRLTLSVEHWRGYLLALGAAIVWSTYSL
                     LTKRLRPFSTAAVGLFCLVSGTLSLACHVLLESRYNPSATEWMRIALLALGPLGIAYF
                     LWDAALKRGDAKTIGALSYLTPLGSTLMLVATGQGTLTALVALAALLIVGGAVLGSVR
                     R"
     gene            2973..4157
                     /gene="bktB"
                     /locus_tag="AC16HB_02155"
     CDS             2973..4157
                     /gene="bktB"
                     /locus_tag="AC16HB_02155"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HIR5605621.1"
                     /translation="MMREVVVVSGVRTAIGTFGGSLKDVPPTQLGARVVKEALARARA
                     DGGEVGHVVFGNVVHTEPRDMYLSRVAALDGGVKDHVPCLTLNRLCGSGLQAIVSAAQ
                     TIMLGDADIAIGGGAENMSRAPYAMPTARFGQRMGDAKIIDMMVAALHDPFQNIHMGV
                     TAENVAAKWGITRQMQDELAVESHRRAALATKEGRFKSQIVPIEIKGKKGPTVFDTDE
                     HFRAEATLEDMAKLKPVFVKENGTVTAGNASGINDGAGAVVLMERSVADKRGLKPLAR
                     LVAYGHAGVDPKYMGIGPVPATQNAMKKAGLTVAQMDVIEANEAFAAQACAVTKDLGA
                     NPAKVNPNGSGISLGHPIGATGAIITVKALYELQRIGGRYALVTMCIGGGQGIAAIFE
                     RL"
     gene            complement(4206..5084)
                     /gene="serB"
                     /locus_tag="AC16HB_02160"
     CDS             complement(4206..5084)
                     /gene="serB"
                     /locus_tag="AC16HB_02160"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR5605622.1"
                     /translation="MTDLVIQSPDLPTAAVEAFKVGCLARRVQRKLNSARLVEIQNDS
                     DTRKVAAALSTYWKCDAAFVSPNLRLSDFRLLALDMDSTLVTIETLDEAATYAGKGEE
                     VAAITAAAMRGEVTDYRESLRRRVAMLAGTDAALLLRVCEEKLQLSPGAERLLSACRA
                     AGLRTLLVTGGFSCFSERLQQRLGFEFMRANELEIVDGRLTGNVSGPPEHDGRIVDAD
                     GKARALRDACAHIGCPTQQAIAIGDGANDLAMLRLAGLSVAFRAQPVVQAQATQTLCY
                     AGLDGVLAWFADSWRQ"
     gene            complement(5088..8531)
                     /gene="mfd"
                     /locus_tag="AC16HB_02165"
     CDS             complement(5088..8531)
                     /gene="mfd"
                     /locus_tag="AC16HB_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR5605623.1"
                     /translation="MTEASRIELALPEIAAGIRFALPLPPGSGDALLIAQYAARARRR
                     GHLVCVICADALDAQRLTDELPYFEPALVVRAFPDWETLPYDILSPHQDLVSDRLEAL
                     YRLITRDANSPIDVLVVPATTALYRLVPTAYVAGHTFFFRQGERLAGDALRARLVPAG
                     YQSVSQVVAPGEFSVRGGLIDLFPMGSTLPYRLDLLDDAIESIRTFDPDTQRSLYPVP
                     EIRLLPGREFPLDEAARAAFRGRWREAFEGDPSRASVYKDVGNGIASAGIEYYLPLFF
                     DATATLFDYLPAESHLVLHGPIESAAHRFLNETSERYRFLARDRERPCLPPARLFMDA
                     EEFFSRAKPHARLALAEGAAPQFATPPAIAVDRKADDPVQRLRDYVERLDGRALLLAD
                     SHGRRETIAQMLREYGLEFADNADFNAFLASDAKLALGVGPLHAGFVLREPHLALLTE
                     TELYATSPRRLRGKARERASNVEAMVRDLAELRVGDPVVHVEHGIGRYLGLESLDLGD
                     GPAEFLHLQYANETKLYVPVAQLHLISRYSGADPDAAPLHHLGGGDWEKAKKKAAKQV
                     RDTAAELLNLYALRAARKGHAFEFKSHDVEAFAEGFGFEETPDQLAAIEAVTRDMGAG
                     FPMDRLVCGDVGFGKTEVALRAAFIAVAGGKQVAVLCPTTLLAEQHTQTFRDRFADWP
                     VKIVELSRFRSPKEVAEAVAGIHAGTIDIVIGTHKLLSEAVRFERLGLVIIDEEHRFG
                     VRQKERLKSLRTEVDVLTLTATPIPRTLALSMEGIREFSVIATAPQRRLAIKTFVRPE
                     SASLIREACLRELKRGGQIYFLHNEVETIANRRARLAELVPEARIEIAHGQMGERELE
                     RVMRDFYQQRFNLLLCTTIIETGIDVPTANTMIIHRADKFGLAQLHQLRGRVGRSHHQ
                     AYAYLMVPDEGGMTKNAEKRLEAIQSLEELGSGFYLAMHDLEIRGAGEVLGESQSGNM
                     QEVGFDLYTQMLNAAVRALRAGREPDLLAPLAAVTEINLHVPALLPADYVGDVHQRLS
                     LYKKLASCDDEDGLFALQEELIDRYGKLPDAARALIETHRLRLVAERLGVKKIDATSE
                     TIVITFVPNPPLDPARLIAMIQRERNMRLAGPERLRIDVKTPSVDARLGQLRDVFRSL
                     T"
     gene            8634..9329
                     /gene="ispD"
                     /locus_tag="AC16HB_02170"
     CDS             8634..9329
                     /gene="ispD"
                     /locus_tag="AC16HB_02170"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR5605624.1"
                     /translation="MNPAPRLIGLIPAAGSGARIGGGVPKQYALLGGRTMLEHSIDAL
                     AGDARVREIVVVVAPDDVRHRTLAPRTGLRFVAVGGASRAESVRNGLRAVDASGEDWV
                     LVHDAARPCLSTTELAGLIESVGNDEAGGLLALPLADTLKRARDGRVDVTLARAALWR
                     AQTPQMFRRDVLLRALDSLPDLEPITDEASAVERLGLHPRLVAGAATNIKVTTADDWP
                     LATAILKAQGRIK"
     gene            9326..9805
                     /gene="ispF"
                     /locus_tag="AC16HB_02175"
     CDS             9326..9805
                     /gene="ispF"
                     /locus_tag="AC16HB_02175"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR5605625.1"
                     /translation="MKFRVGQGFDVHRLVTGRRLVLGGVEIPFTQGLAGHSDADVLLH
                     AITDALLGAAGLGDIGRMFPDTDAQYAGADSVVLLKTATARVCAAGWRIENIDGTVIA
                     QQPRIAPYVDAMRARIAGAVGIEVGAVGIKGKTTEQLGFTGRGEGIAALAVALLVRD"
     gene            complement(9802..11127)
                     /locus_tag="AC16HB_02180"
     CDS             complement(9802..11127)
                     /locus_tag="AC16HB_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5605626.1"
                     /translation="MKLRSPSLFWRTFLLILLLIVASLLAWLQSFRVFEREPRAQRIA
                     QHIVSIVNITRAALVYSDPAERRQLLAELTENVGIRVVPLEPADIVEPLPDVAVVPMV
                     ADRIRAQLGPETRIGGEVNGVPGVWVSFDIEGDAYWVFIEGDLLTRDVGTQWIGWAIV
                     ATLLSLLAAVVITRVVNQPLQRLSRAARELGAGQHPRALPETGPSEIRTVNQSFNRMV
                     SDLGKLEQDRAVLLAGISHDLRTPLTRLRLEVEINDLPEETRRAMIGDLEQMDAIVGQ
                     FLDYARAKPQQATEPIDLSTLVDDALAHSRLMHTEAATIARDVEHGIVVQGHRTELLR
                     AIDNLLTNADRYGRTPEVDRLELAVSLTREGAVAVLSIADQGPGVATERVQQLMRPFE
                     RGDAARSEVKGAGLGLAIVQRIARMHGGSVELKANQPRGLRAELRLPVA"
     gene            complement(11129..11854)
                     /gene="ompR"
                     /locus_tag="AC16HB_02185"
     CDS             complement(11129..11854)
                     /gene="ompR"
                     /locus_tag="AC16HB_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmolarity response regulator transcription
                     factor OmpR"
                     /protein_id="HIR5605627.1"
                     /translation="MHRSGPSKVLVVDDDPRLRDLLRRYLTDNGFTVYTAESGQAMNK
                     LWLRERFDVLILDLMLPGEDGLSILRRLRGANETTPIIMLTAKGEDVDRIVGLEMGAD
                     DYLPKPFNPRELLARINAVLRRKGADEAPGAPAQEPKTVEFGGFVLDLATRTLTKDGE
                     QVPLTTGEFAVLKVFARYPRTPLSREKLMEMARGREYEAFDRSLDVQISRLRKMIEPD
                     PSKPKYIQTVWGLGYVFIPDGQT"
     gene            12025..12486
                     /locus_tag="AC16HB_02190"
     CDS             12025..12486
                     /locus_tag="AC16HB_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR5605628.1"
                     /translation="MRLRAFIASVLLLIAADGLRAQGVRDEVLRNMTPQQRENLWQSM
                     TPQQRAEFWRRLTPEQRQAIRQQMSPDQRDAMRQRMLEQRERHGGPGTSPLAESAAPT
                     PGVGPGAGAGPRRLSPEERQRLREQIMESARDLREDRRPRMLMERHKGRER"
     gene            12530..13249
                     /gene="tsaB"
                     /locus_tag="AC16HB_02195"
     CDS             12530..13249
                     /gene="tsaB"
                     /locus_tag="AC16HB_02195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5605629.1"
                     /translation="MPPTPTILAIDTAADVCSVALACAGGVREHSETVGHRHSERVLP
                     MVRTLLASERVELSDCDALAFGAGPGAFTGLRIACGVVQGLAYGAGKPVIAVGNLEAL
                     AWLALQRHTDAARVLCANDARMQEAYVAVYQRDGEDLIELSAPALAAGSELAELIDHW
                     AADLVVGDAPVVFSDRLAGVAASTRLVTLRATATAICERAVRRWTAGDGADAKYAAPL
                     YVRNHVALTIEERRARQGRAA"
     gene            13246..13713
                     /gene="rimI"
                     /locus_tag="AC16HB_02200"
     CDS             13246..13713
                     /gene="rimI"
                     /locus_tag="AC16HB_02200"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5605630.1"
                     /translation="MSAVVRPAEDRFAPMRAEDLDEVMAIENDLYPFPWTRGNFRDSL
                     RAGYSVWTLRGPEGELIAYAVVMIALDEAHLLNLSVARHQQRRGQGWRTLDWMASVAR
                     DHGARSMLLEVRPSNSTGQRLYASYGFEPIGRRRDYYPAVGGREDAIVMRIAL"
     gene            13724..14437
                     /locus_tag="AC16HB_02205"
     CDS             13724..14437
                     /locus_tag="AC16HB_02205"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5605631.1"
                     /translation="MTLDPRRAREWDAMGLGPRWQLRASAAEAPPPAAPSDARADTIR
                     RMDWDTLQQVVAGCTACALCKTRKQTVFGVGNPQAHWMLIGEAPGAEEDARGEPFVGQ
                     AGRLLDNMLSSVGLSRSGEGAHAVYIANVLKCRPPGNRNPDPAEVALCEPFLLRQVAL
                     VQPKLILVMGRFAAQSLLGTEATIASLRGRVHSIDVEGRRVPVIVTYHPAYLLRNLAD
                     KAKSWTDLCLARDVYARPA"
     gene            complement(14448..15770)
                     /gene="lplT"
                     /locus_tag="AC16HB_02210"
     CDS             complement(14448..15770)
                     /gene="lplT"
                     /locus_tag="AC16HB_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HIR5605632.1"
                     /translation="MNRGFYVIMAAQFFSSLADNALLIAAIALLRDINAPEWMTPMLK
                     LFFFVSYVMLAAFVGAFSDARPKGQVMLITNGVKIVGCLVMLFGLHPLAAYAVVGLGA
                     AAYSPAKYGILTELLPPEKLVVANGWIEGLTVGSIILGFVLGGVLVSPHVAASLTSFD
                     FPLIDFDIDQPGEAAIGVIALLYAIAAFINTRIPDTGARYPRQERSPWRLILDFSHCV
                     VTLWRDKLGQISLAVTTLFWGAGATLQFIILKWCEQVLGLSLSQGAIMQAVVAIGIAI
                     GSVWAAARVSLKRSLSVLPVGIAMGLVVMSMALIDFSPLRSSGLPPGTLALSPALLAA
                     SALMVIVGALAGYFVVPMNALLQHRGYVLLSAGHSIAVQNFNENLSILVMLGVYATLI
                     WLDLSIDTIIILFGLFVATTMALIMLRHQYNQRQFDSVALIGEGRRVH"
     gene            15896..16975
                     /gene="alr"
                     /locus_tag="AC16HB_02215"
     CDS             15896..16975
                     /gene="alr"
                     /locus_tag="AC16HB_02215"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5605633.1"
                     /translation="MPRPIRATVDLAAMRHNLARARQEAGARRVWAVVKANAYGHGIE
                     RAVRAFADADGLALLDLDEAQRARDAGWRKPILLLEGFFEPGDLDVVDALDLSTVVHQ
                     REQVEMLAAHRPGRAIEVYVKLDTGMRRLGFGDDAMEVVARLQSLPHVRVAALATHLA
                     NADRSASGPAAVADQLRAFAAATRGWQGAVSLANSAALFLRPEVGGDSVRPGIALYGG
                     RPRAGQPATQLGLRPAMRLRSRLLEVRDLQAGDAVGYGSRWIAQRPARIGVVACGYAD
                     GYPRVAPDGTPVWIAGKIVPLAGRVSMDMITVDLADAMHARVGAEVELWGEHVPIDDV
                     AEQAGTVGYELMCALARRVAVDEIG"
     gene            16979..>17718
                     /locus_tag="AC16HB_02220"
     CDS             16979..>17718
                     /locus_tag="AC16HB_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7125 family protein"
                     /protein_id="HIR5605634.1"
                     /translation="MAKVKTQFVCSECGGTSPRWQGQCPQCNAWNTLVEARVEAGSRR
                     YAPLAPVASVQLLSEIEAREVGRTSTGIEEFDRVLGGGLVEGGVVLIGGDPGIGKSTL
                     LLQALAAMAGGQNVLYVSGEESAGQIALRARRLGVDGRNVRFMAEIDLGRILGALETQ
                     RPQVAVIDSIQTLCWSELTSAPGSVAQVRECAAQLTRIAKASGITLVLVGHVTKEGAL
                     AGPRVLEHMVDTVLYFEGDTHSSFRLIRA"
BASE COUNT         2945 a         5885 c         6144 g         2744 t
ORIGIN      
        1 ggtgcggtgg cgcagcggcg aaagtcgacc atcccgcgaa gtggctagaa tccccttcgg
       61 acactctgtt gccgagggac ggcgccgcgc atgcggcgcc gatgattaca aggaagtcgt
      121 ttgatgccaa ccgcccagcg cttgatcaag aagtatccga atcgccgcct gtacgacacg
      181 cagaccagca cctatatcac gctggcggac gtgaaggagc tggtgctcga gcagaccgat
      241 ttccaggtgg tcgacgccaa gacgggggag gacctgacgc gccagatcct gctgcagatc
      301 attctcgaag aggagcagga aggccagccg atgttcacgc cgccggtgct gtcgcagatc
      361 atccgcagct acggcaatgc gatgcagggc atgatggggt cgtacctcga gaagaacgtg
      421 caggcgttca tcgacatcca gaacaagctg caggagcaga gcaaggcgtt ttacgacagc
      481 aacaagatcg gcccggagat gtggacgcag ttcgtcaaca tgcaggcgcc gatgattcag
      541 tcgatgatgt cgaactacat cgagcaatcg aagaacctct tcgtgcagat gcaggagaac
      601 atgcagaatc aggcgcgctc gatgttcaac accttcccgt tccagggcgg cgacaagaag
      661 tagcgccacc gcatcacccg caatgaccgt gtcgtccctc gcgcggcgcg cgcgcaagcc
      721 cgtcgcgccg cgcggcgttc ctaccgtcgg tttcgtctcg ctcggctgcc cgaaggcgct
      781 ggtcgactcg gagcgcatcg tcacgcagct gcgcgcggag ggctacgcgt tgagctcgac
      841 ctacgatagc gcggacctcg tgatcgtcaa cacctgcggc ttcatcgacg ccgcggtgca
      901 ggagtcgctc gacgcgatcg gcgaggcgct gcacgagaac ggcagagtga tcgtcaccgg
      961 ctgcctcggc gcgaagaccg acggcggtgc gaatctggtc gccaagattc accccaaggt
     1021 gctggcggtg accggaccgc acgcgacgga cgaggtgatg ggccacgtgc atcaccacct
     1081 gcccaagccg cacgacccgt tcacggatct ggtgcccccg gccggcatca agctgacccc
     1141 gaggcactac gcgtatctga agatcgccga gggctgcaac catcactgca cgttctgcat
     1201 catcccgtcg atgcgcgggc cgctcgtctc gcggccgatc ggcgaggtga tgcacgaggc
     1261 cgaagggctg gtgcagcaag gcgtcaagga aatcctcgtc gtgtcgcagg acacgagcgc
     1321 ctacggtgtg gacgtgaagt acaagctcga cttcgtcggc gggcgcgcgg tgaagaccag
     1381 gatgacgatg ttggcgcagg agttgggcag gctcggcgtc tgggtgcggc tgcactacgt
     1441 gtacccgtat ccgcacgtcg acgaggtgat tccgctgatg gcgacgggca aggtgctgcc
     1501 ctatctcgat gtcccgttcc agcacgcgca cccggcggtg ctgaagcgga tgaagcgacc
     1561 cgccgacggc gagcggaaca tcgaacgcat ccgcgcctgg cgggcgatct gccccgacct
     1621 cacgattcgc tcgaccttca tcgccggctt ccccggcgag acgaaagatc agttcgaata
     1681 tttgctggac ttcctgcgcg aagcgcggct cgatcgcgtc ggctgcttcg cctattcgcc
     1741 cgtcgcgggt gccgcggcca acggactcga cggcgcgctg ccggacgagg tgcgggacga
     1801 acggcgcgcg cgcttcatgc aggtgcagga ggcgatctcg cgcgaacgac tgaggcgcaa
     1861 ggtcggcagc gtgcagcggg tgctggtcga cgaggtcatg gccgaaggcg cgcgcgcgcg
     1921 ctcgaccgcg gatgcgccgg agatcgacgg cgtcgtgcac gtcaaaacca gtcgcaagct
     1981 gaaagtcggc gaattcttcg atgtgcgcat cgatgccagc gacgcgcacg atctatcggg
     2041 ccggctggtc tagcggcgca ccgagccgag caccgcaccg ccgacgatca gcagcgcggc
     2101 cagcgccacc agcgcagtga gcgtgccctg gccggtggcc acgagcatca gcgtcgagcc
     2161 gagcggcgtc aggtacgaca gtgcgccgat cgtcttcgca tcgccgcgct tcagcgccgc
     2221 gtcccacaga aagtaggcga tgcccagcgg cccgagcgcc agcagagcga tgcgcatcca
     2281 ttccgttgca cttgggttgt agcgggactc cagcagcacg tggcacgcga gcgaaagcgt
     2341 gcccgacacc aggcagaaca gaccgaccgc cgcggtggaa aagggccgca gtcgcttggt
     2401 cagcagcgaa tacgtggacc acacgatcgc cgcacccagc gccagcagat agccccgcca
     2461 atgctcgacc gacagcgtca aacggccgcc ggtgatcacc agcgcagcgc cggaaaaccc
     2521 catcagcgca ccgatcacgt ggcgcggcgc cagccgctcg ccgggcagca gcagcggcgc
     2581 cagcacgacg atcagcagcg gccagaggta gttgatcagg ttggcctcca ccggcggcgc
     2641 cagtcgcagc gcagtgaaca gcaacaaatg aaatccgaac agcccataca ccccgaccgc
     2701 gagcgtggcg gggggcacgc gccatgcgcg ccaggtcggc agtgtcacca gcccgacaat
     2761 cgtcatcggc gctcccacga gcaagaacgg cggcaccgac ttcagcgaga ccgcgagcgc
     2821 ggccaccgac atccacaacg cgatggcgcc gagcgcgagc acggcagggg gatagcggaa
     2881 catcgggacg gcaagcttga tcggacgcag acgcggccgt tgacaaggcg gtatcgtgcc
     2941 ggttctggtc aggccggagc ccaaaggagc ggattatgcg agaagtcgtc gtcgtcagcg
     3001 gtgtgcgcac agcgatcgga acctttggcg gcagcctgaa ggacgtgccg ccgacgcaac
     3061 tcggtgcgcg ggtcgtcaag gaggcgctcg cacgcgccag ggccgacggc ggcgaggtcg
     3121 gccacgtcgt tttcggcaat gtcgtgcaca ccgagccgcg cgacatgtat ctgtcgcggg
     3181 ttgccgcgct cgatggcggg gtgaaggatc acgtgccctg cctgacgctg aaccggctgt
     3241 gcggcagcgg gctgcaggcc atcgtcagcg ccgcgcagac gatcatgctc ggcgacgccg
     3301 acatcgccat cggcggcggt gccgagaaca tgtcgcgcgc gccgtatgca atgccgaccg
     3361 cgcgcttcgg ccagcggatg ggcgacgcga agataatcga catgatggtc gccgcactgc
     3421 acgacccctt ccagaacatc cacatgggcg tgaccgcgga aaatgtcgcc gccaagtggg
     3481 gcatcacgcg ccagatgcag gacgaactgg cggtcgagag ccatcgccgg gctgccttgg
     3541 cgaccaagga gggccgtttc aagtcgcaga tcgttccgat cgagatcaag ggcaagaagg
     3601 gcccgaccgt gttcgacacc gacgagcatt tccgcgccga ggcaacgctg gaggacatgg
     3661 cgaagctgaa gcccgtgttc gtgaaggaga acggcaccgt caccgctggc aacgcctccg
     3721 gcatcaacga cggcgccggc gccgtcgtgc tgatggagcg cagcgtcgcc gacaagcgcg
     3781 ggctgaaacc gctggcgcgg ctggtcgcct acggtcacgc gggcgtcgat ccgaagtaca
     3841 tgggcatcgg cccggtgccg gccacgcaga acgcgatgaa gaaggccggc ctgacggtcg
     3901 cgcagatgga cgtgatcgag gccaacgagg cgttcgctgc ccaggcctgc gcggtgacca
     3961 aggacctcgg ggcgaatcct gcgaaggtaa acccgaacgg cagcgggatc tcgcttgggc
     4021 atccgatcgg tgccaccggc gcgatcatca ccgtcaaggc gctatacgaa ctgcagcgca
     4081 tcggtggccg ctacgcgctg gtgacgatgt gcatcggcgg cgggcaggga atcgcggcaa
     4141 tcttcgagcg gttgtaggca ccgcgcagcg cgacccggaa gccggccttc gcgccggctt
     4201 ccttttcatt gccgccagga atcggcgaac cacgcgagta cgccgtcgag ccccgcgtag
     4261 cacaatgtct gcgttgcctg cgcctgcacc accggctggg cgcgaaaggc cacggacaga
     4321 ccggcgagtc tcagcatcgc caggtcattg gcaccgtcgc cgatcgcgat cgcctgctga
     4381 gtcggacacc cgatgtgcgc gcacgcgtcg cgcagcgcgc gcgccttgcc gtcggcatcg
     4441 acgatcctgc catcgtgttc cggcgggccc gacacgttcc cggtcaggcg gccatcgacg
     4501 atctccagtt cgttggcgcg catgaattcg aagccgagcc gttgctgcaa gcgctcgctg
     4561 aagcagctaa acccgcctgt gacgagcagg gttctcagac ccgcggcccg gcaggcggat
     4621 agaagccgtt ccgcacccgg gctgagctgc agcttctctt cacagacccg cagcagcagc
     4681 gcggcatccg tgcccgccag cattgccacg cgccggcgca ggctctccct gtagtcggtg
     4741 acctcgccac gcatcgcggc cgcggtgatc gctgcgactt cctcgccctt gccggcgtag
     4801 gtggcagcct cgtcgagcgt ctcgatcgtc acgagggtcg agtccatgtc gagcgcgagc
     4861 agcctgaagt cggagagcct caggttcggc gacacgaatg cagcgtcgca cttccagtac
     4921 gtcgacagcg ccgcggcaac ctttcgcgtg tcgctgtcgt tctgtatctc gaccagccgc
     4981 gcgctgttca gtttccgttg cacgcgccgc gccaggcacc cgaccttgaa ggcctcgacc
     5041 gcggcggtcg gcagatccgg ggactggatg acgagatcgg tcacagttca ggtgagtgac
     5101 ctgaagacgt cgcggagttg tcccagccgc gcatcgacgc tcggcgtctt gacgtcgatg
     5161 cgtaggcgct cggggcccgc cagccgcatg ttcctctcgc gctggatcat ggcgatgagt
     5221 cgagcgggat cgagcggcgg attcggaaca aatgtgatca cgatcgtctc gctggtggcg
     5281 tcgatcttct tcacgccgag tctctcggcg acaagccgca gccggtgcgt ctcgatgagc
     5341 gcgcgcgccg catcgggtag cttgccgtag cggtcgatca gctcttcctg cagcgcgaac
     5401 agcccgtcct cgtcgtcgca cgacgcaagc ttcttgtaca gcgacagacg ctggtgcacg
     5461 tcgccgacgt agtctgccgg cagcagcgcc ggcacgtgca ggttgatctc ggtcaccgct
     5521 gcgagcggcg ccagcagatc cggttcgcgc ccggcgcgca gcgcgcgcac cgcggcgttc
     5581 agcatctgcg tgtacaggtc gaagccgacc tcctgcatgt tcccgctctg cgattcgccg
     5641 agcacctcgc cggcgccgcg gatctcgagg tcgtgcatcg ccagatagaa gccgctgccg
     5701 agctcttcca ggctctggat cgcttcgagc cgcttctccg cgttcttcgt catgccgcct
     5761 tcgtccggca ccatgagata cgcatacgcc tggtggtgcg agcggccgac gcggccgcgc
     5821 agctggtgca gctgcgccag cccgaatttg tcggcgcgat ggatgatcat cgtgttcgcg
     5881 gtcggcacgt cgataccggt ctcgatgatg gtcgtgcaca gcagcaggtt gaatctctgc
     5941 tgatagaaat cgcgcatcac gcgctccagt tcccgctcgc ccatctgccc gtgcgcgatc
     6001 tcgatgcgcg cctcgggcac cagttccgcc agccgcgccc gccggtttgc gatcgtttcg
     6061 acctcgttgt gcaggaagta gatctggccg ccgcgcttca gctcccgcag gcacgcctcg
     6121 cggatcaggc tcgccgactc cggtcgcacg aacgtcttga tcgccagccg ccgctgcggg
     6181 gcggtggcga tcacgctgaa ctcgcggatg ccttccatcg acagcgccag cgtgcgcggg
     6241 atcggggtgg cggtcagcgt cagcacatcg acctcggttc gcagcgattt cagcctctcc
     6301 ttctgccgca cgccgaagcg gtgctcctcg tcgatgatga ccagccccag gcgttcgaac
     6361 cgcaccgcct cgctcaggag cttgtgcgtg ccgatcacga tgtcgatcgt gccggcatgg
     6421 atgccggcaa ccgcttcggc gacttccttc ggggagcgga agcgtgaaag ctccacgatc
     6481 ttcaccggcc agtctgcgaa gcggtcgcga aacgtctgcg tgtgctgttc cgccagcagc
     6541 gtggtcgggc acagcaccgc gacctgcttg ccgccggcca cggcgatgaa ggcggcgcgc
     6601 agcgcgacct cggtcttgcc gaagccgacg tcgccgcata cgagccggtc catcggaaag
     6661 cccgcgccca tgtcgcgtgt gaccgcctcg atcgcggcca gttggtcggg cgtttcctcg
     6721 aagccaaatc cctcggcgaa cgcctcgacg tcatgcgact tgaattcgaa cgcgtggccc
     6781 ttgcgcgcgg cacgcagcgc gtacaggttc agcagttccg ccgccgtatc gcgcacctgc
     6841 ttggcggcct tcttcttggc cttctcccag tcgccaccgc cgaggtggtg caagggtgcc
     6901 gcgtcgggat ccgcaccgct gtagcgcgag atcaggtgca gctgcgcgac cggcacgtac
     6961 agcttggttt cgttcgcgta ctgcagatgc aggaattcgg ccggtccgtc gccgaggtcg
     7021 agcgattcca gtccgaggta acggccgatg ccgtgctcga cgtggacgac cggatcgccg
     7081 actcgtagct cggcgaggtc gcgcaccatc gcttcgacat tcgacgcgcg ctcgcgcgcc
     7141 ttgccgcgca ggcggcgcgg gctggtcgcg tacagctccg tctcggtgag gagcgcgagg
     7201 tggggttcgc gcagcacgaa ccccgcgtgc agcgggccga caccgagcgc gagtttcgcg
     7261 tcgctcgcca ggaacgcgtt gaagtcggcg ttgtcggcga actcgagccc gtactcgcgc
     7321 agcatctgcg cgatcgtctc gcgccggccg tgcgagtcgg cgagcagcag cgcacgtccg
     7381 tccaggcgtt cgacgtaatc gcgcagtcgc tgcaccggat cgtcggcctt gcggtcgacg
     7441 gcgatcgcgg gcggcgtcgc gaactgcggg gccgcgccct cagccagcgc cagtcgcgca
     7501 tgcggcttgg cacgcgagaa gaattcctcg gcgtccatga agagccgcgc cggcggcagg
     7561 cacggccgct cgcgatcgcg cgcgaggaag cgatagcgct cgctggtctc gttcaggaat
     7621 cgatgcgcgg ccgactcgat cggaccatgc agcaccaggt gcgactccgc gggcaggtaa
     7681 tcgaacagcg tggcggtcgc gtcgaagaac aagggcaggt agtactcgat gccggcactg
     7741 gcgatgccat tgccgacgtc cttgtagacg ctggcgcggc tgggatcgcc ctcgaacgcc
     7801 tcgcgccagc ggccgcggaa tgcggcgcgc gcggcctcgt cgagcgggaa ctcgcgtccg
     7861 ggcaagaggc ggatctccgg caccggatag aggctgcgct gcgtgtcggg gtcgaacgtg
     7921 cggatcgatt cgatcgcatc gtcgagcaga tccagccggt acggcagcgt cgaacccatc
     7981 gggaagaggt cgatcagccc gccacgcacc gaaaactccc cgggcgcgac gacctgcgac
     8041 acgctctggt agccggccgg taccagccgc gcgcgcaatg cgtcacctgc cagccgctcg
     8101 ccctggcgaa agaagaacgt gtggcccgcg acgtatgccg tcggtaccaa acgatacagc
     8161 gcggtcgtcg cgggcacgac cagcacgtcg atgggcgagt tcgcgtcgcg cgtgatcagg
     8221 cggtacagtg cctcgaggcg gtccgaaacg aggtcctggt gcggcgacag gatgtcgtag
     8281 ggcagcgttt cccagtcggg aaacgcgcgt accacgagtg ccggttcgaa gtagggcagc
     8341 tcgtcggtga gccgctgcgc atcgagtgcg tcggcgcaga tcacgcacac cagatggccg
     8401 cgcctgcgcg cgcgcgccgc atactgcgcg atcaacaggg cgtcgccgga gccaggaggc
     8461 agcgggaggg caaagcgaat ccccgccgca atctcgggca gcgcaagttc gatccgcgat
     8521 gcttcggtca gtacgtccac ggactgcaca aacaacacgc ggcggccgaa tccgatccga
     8581 ccgccgcgaa cgcgccgcta ttataggtcc gcactcttct tcgactgctt acgttgaacc
     8641 cagcgccgcg actgatcgga ctgattcccg ctgccggcag cggcgcgcgt atcggcggcg
     8701 gcgttcccaa gcagtacgcg ttgctgggcg gccgcacgat gctcgaacat tcgatcgatg
     8761 cgctggccgg cgatgcgcgc gtgcgtgaga tcgtggtcgt ggtcgcgccg gacgacgtgc
     8821 ggcatcggac gctcgcgccg cggaccggcc tgcgtttcgt tgcggtcggc ggcgcgtcgc
     8881 gcgcggaatc cgtgcgcaac ggcctgcggg cagtcgatgc aagcggcgag gattgggtcc
     8941 tggtgcacga tgccgcgcgg ccgtgcctgt cgaccacgga gctggcgggt ttgatcgaat
     9001 ccgtgggcaa cgacgaggcg ggcggactgc tggcattgcc gcttgccgac acgctgaaac
     9061 gcgcgcgcga cgggcgggtc gacgtgaccc tggcgcgcgc ggcgctgtgg cgcgcccaga
     9121 cgccgcagat gtttcgccgc gacgtgctgc tgcgcgcgct cgattcgttg ccggacctcg
     9181 agccgattac cgacgaagcg agcgccgtcg agcggcttgg cctgcatccg cgactggtcg
     9241 cgggcgcggc gaccaacatc aaggtcacga ccgcggacga ttggccgctc gcgactgcga
     9301 tcctcaaggc acaggggcga atcaaatgaa gttccgcgtc gggcagggct tcgatgtgca
     9361 caggctggtc accggacggc ggctcgtgct ggggggtgtc gagattccgt tcacgcaagg
     9421 cctcgccggc cattccgatg ccgacgtgct gctgcacgcg atcactgacg cgttgctcgg
     9481 cgcagccggg ctgggcgaca tcggccgcat gtttccggac accgatgcgc agtacgctgg
     9541 cgccgacagc gtcgtgctgc tgaagacagc gaccgcgcgg gtatgcgccg ccggctggcg
     9601 gatcgagaac atcgacggca ccgtgatcgc gcagcagccg aggatcgcac cttacgtgga
     9661 tgcgatgcgg gcacggatcg ccggcgcggt cgggatcgag gtcggcgcgg tcggcatcaa
     9721 gggcaagacg accgagcagc tcggcttcac cggccgcggc gagggcattg cggcgctggc
     9781 ggtggcactg ctcgtacgcg actaagcgac gggtagccgc agttcggcgc gcagcccgcg
     9841 cggctggttg gccttcagct cgaccgatcc gccgtgcatg cgcgcgatgc gctgaacgat
     9901 cgccagcccg agtcccgccc ctttgacctc gctacgcgcc gcatcgccgc gctcgaaggg
     9961 gcgcatcaac tgctggacgc gttccgtcgc cacgcccgga ccttggtcgg cgatcgacag
    10021 cacggcaaca gcgccttcgc gcgtgaggct caccgcgagc tccagccgat cgacctccgg
    10081 cgtgcggccg taacggtcgg cgttggtcag caggttgtcg attgcgcgca gcagttcggt
    10141 gcgatggccc tgcacgacga tgccgtgctc gacgtcgcgc gcgatcgttg ccgcttccgt
    10201 gtgcatcagg cggctatggg ccagtgcgtc gtcgaccagg gtcgacaggt cgatcggctc
    10261 cgtcgcctgc tgcggtttgg cgcgggcgta gtccaggaac tggccgacga tcgcatccat
    10321 ctgttcgaga tcgccgatca tcgcccggcg cgtctcctcc ggcaggtcgt tgatctcgac
    10381 ctcgagccgc agcctcgtga gcggcgtgcg caggtcgtgc gagatgccgg ccagcaacac
    10441 cgcgcgatcc tgttccagtt tgccgaggtc gctgaccatg cggttgaagc tctggttgac
    10501 ggtgcggatc tcgctcggcc cggtctctgg cagggcgcgt gggtgctggc cggcgcccag
    10561 ttcgcgtgcg gcgcgcgaca accgctgcag cggctggttg accacgcgcg tgatcaccac
    10621 cgcggccagc agcgacagca gcgtggcgac gatggcccag ccgatccact gcgtgccgac
    10681 gtcgcgcgtc agcaggtcgc cctcgatgaa gacccagtat gcgtcgccct cgatgtcgaa
    10741 gctgacccag acgccgggca cgccgttgac ctcgccgccg atccgcgtct cggggccgag
    10801 ttgggcacgg atccgatcgg ccaccatcgg cactaccgcg acgtcgggca gcggttcgac
    10861 gatgtcggca ggctccagcg gcacgacacg gatgccgacg ttctcggtca gctccgccag
    10921 cagctgccgg cgctcggccg gatccgaata gaccagtgcc gcgcgcgtga tgttgacgat
    10981 ggaaacgatg tgctgcgcga tgcgttgcgc gcgcggctcg cgctcgaaca cgcgaaagct
    11041 ctgcaaccag gccaacaggc tggcgacgat cagcagcagg atcagcagga aggtgcgcca
    11101 gaacaggctt ggcgagcgca gcttcaactc aggtctgccc gtcggggatg aagacgtagc
    11161 ccaggcccca cacggtctga atgtacttgg gcttggacgg gtcgggctcg atcatcttgc
    11221 gcaggcgcga gatctgcacg tcgagcgagc ggtcgaacgc ctcgtattcg cgaccgcgcg
    11281 ccatctccat cagcttttcg cgcgacagcg gcgtgcgtgg gtagcgcgcg aagactttca
    11341 gcaccgcgaa ttcgcccgtc gtcagcggca cctgttcgcc atccttggtc agcgtgcgcg
    11401 tggcgaggtc cagcacgaag ccgccgaact cgaccgtctt cggctcctgt gccggcgccc
    11461 cgggcgcctc gtccgcgccc ttgcgccgca ggaccgcatt gatgcgcgcc agcagctcgc
    11521 gcggattgaa cggcttcggc aggtagtcgt ccgcgcccat ctcgaggccg acgatgcggt
    11581 cgacgtcctc gcccttggcg gtcagcatga tgatcggcgt tgtctcgttg gcgccgcgca
    11641 gccgccgcag gatcgacagc ccgtcctcgc ccggcagcat caggtccagg atcagcacgt
    11701 cgaagcgttc gcgcagccac agcttgttca tcgcctggcc actttccgcc gtgtagacgg
    11761 tgaagccgtt gtcggtcagg tagcggcgca gcaggtcgcg caagcgcgga tcgtcgtcga
    11821 cgaccagaac tttcgaaggg ccggatcggt gcatggtcga tctccagggt ctgccgggcg
    11881 cgatgctacg gaccgtctcc gacgtgtcgc cgcgcggtcc acaaagtgtt acagaattta
    11941 ccgcgcccgc cgtggtctcc gagtcccgaa gtgagtccat tcggacgttg aacgcatacc
    12001 attcgatttg tgattcggaa ccccatgcgc ctgcgcgcct tcatcgccag cgttctgctg
    12061 ctgatcgcgg ccgacggcct gcgcgcgcaa ggcgtgcgcg acgaggttct gcgcaacatg
    12121 acgccgcagc agcgcgagaa cctgtggcag tcgatgacgc cgcagcagcg cgccgagttc
    12181 tggcgccggc tcacgcccga gcagcggcag gcgatccggc agcagatgtc gcccgaccag
    12241 cgcgacgcaa tgcggcagcg gatgctcgag cagcgcgaaa ggcacggcgg gccggggact
    12301 tcgccgctgg cagaaagcgc cgcgccgacg ccgggtgtgg gtccgggcgc cggagccgga
    12361 ccgcgccgac tgtcaccgga ggagcgccag aggctgcgcg aacagatcat ggaatccgcg
    12421 cgtgacctgc gcgaagatcg acgcccccgc atgttgatgg agcggcacaa gggccgcgaa
    12481 cgctgagcag agcccaggcg cagcgggggc gccgctagaa tcggtccgcg tgcccccgac
    12541 cccgacgatc ctcgccatcg acaccgccgc cgacgtctgt tcggtcgcgc tggcgtgcgc
    12601 cggtggggtc cgcgaacaca gcgagacggt cggccatcgt cactcggagc gcgtgctgcc
    12661 gatggtgcga acgctgctcg cctcggagcg cgtcgagctt tccgactgcg atgcgctcgc
    12721 cttcggcgcc gggccgggtg ctttcacggg gctgcggatc gcctgcggcg tcgtgcaggg
    12781 ccttgcctac ggagctggca aaccggtgat cgcggtggga aacctcgagg cgctggcatg
    12841 gctcgcgttg cagcggcaca cggatgcagc gcgcgtgctg tgcgcgaacg acgcacgcat
    12901 gcaggaagcg tacgtcgccg tctaccagcg tgacggcgaa gacctgatcg aactttcggc
    12961 gccggccctg gcggcgggtt ccgagctggc agaactcatc gatcactggg cagccgatct
    13021 tgtcgtgggc gacgcgccgg tcgtgttctc ggaccgactc gcaggtgtcg ccgcatcgac
    13081 gcggctcgtg acgctgcgcg cgaccgccac cgccatttgc gagcgagcgg tgcggcgctg
    13141 gactgcgggt gacggcgccg acgccaagta cgcggctccg ctgtacgtac gcaaccatgt
    13201 cgcgctgacg atcgaagaac gccgcgcgcg gcagggcagg gccgcatgag cgccgtcgtt
    13261 cgtccggccg aggaccggtt cgcgccgatg cgcgcggagg atctcgatga ggtcatggcg
    13321 atcgagaacg acctttaccc gtttccgtgg acgcgcggaa atttccgcga ttcgctcaga
    13381 gccggttaca gcgtgtggac gctgcgcggt cccgagggtg agctgatcgc atacgccgtc
    13441 gtgatgatcg cgctcgacga ggcgcatctg ctgaacctgt cggtcgcccg gcatcagcag
    13501 cgccgcggcc agggctggcg cacgctcgac tggatggcat cggtggcgcg cgatcatggc
    13561 gcgcgctcga tgctgctcga ggttcggccc agcaattcga ccggccagcg tctgtacgcg
    13621 agctacggct tcgagccgat cggccgccgg cgcgattatt accccgccgt cggcggtcgc
    13681 gaggacgcga tcgtgatgcg catcgcactg tgagcagcgc cggatgacgc tcgatccgcg
    13741 ccgggcacgc gaatgggacg cgatgggact cggcccgcgc tggcagctac gcgcgtccgc
    13801 cgctgaagcg ccgccgcccg ccgcgccgtc ggatgcgcgt gccgacacga tccggcgcat
    13861 ggactgggac accttgcagc aagtggtggc gggctgcacc gcctgtgcgc tgtgcaagac
    13921 gcgcaagcag accgtgttcg gcgtcggcaa tccgcaagca cactggatgc tgattggcga
    13981 ggcgccgggc gcggaagagg acgcccgggg tgagcccttc gtgggccagg ccgggcgcct
    14041 gctcgacaac atgctgtcat cggtcgggct gtcgcgcagc ggcgaaggcg cgcacgcggt
    14101 ctacatcgcc aacgtgctga agtgccgtcc gcccggcaat cgcaatccgg atcccgcgga
    14161 ggttgcgctg tgcgaaccgt tcctgctgcg tcaggtggcg ctggtgcagc cgaaactcat
    14221 cctcgtgatg gggcgcttcg cggcgcagtc gttgctcggc accgaggcga cgatcgcgag
    14281 cctgcgcggg cgcgtgcatt cgatcgacgt cgaaggtcgc cgcgttccgg tcatcgtcac
    14341 ctatcacccc gcctacctgc tgcgtaacct cgccgacaag gcgaagtcct ggaccgatct
    14401 gtgcctggcg cgcgacgtgt acgcacggcc ggcgtgaccg ccgcgcctca gtgcacgcgg
    14461 cgcccttcgc cgatcagcgc gaccgaatcg aactggcgct ggttgtactg gtgacgcagc
    14521 atgattaagg ccatcgtggt cgcgacgaac aagccgaaca ggatgatgat cgtatcgatg
    14581 ctcaggtcga gccagatcag cgtggcgtag acgccgagca tcaccaggat gctgaggttc
    14641 tcgttgaagt tctgcaccgc gatcgagtgc ccggccgaca gcagcacata accgcggtgc
    14701 tgcagcagcg cattcatcgg cacgacgaaa taccccgcca gcgcgccgac gatgaccatc
    14761 agcgccgagg cggccagcag ggccgggctc agcgccaggg ttccgggcgg cagcccactg
    14821 ctgcgcagtg gcgagaagtc gatcagcgcc attgacatca cgaccaggcc catcgcaatg
    14881 ccgacgggca acacgctcag cgatcgcttc agcgatacac gcgcggcggc ccagacggaa
    14941 ccgatcgcaa tgccgatcgc gaccacggcc tgcatgatcg cgccctgcga aagcgacagc
    15001 ccgagcacct gttcgcacca tttcaggatg atgaactgca gagtcgcgcc ggcgccccag
    15061 aacagcgtcg tgaccgcgag cgagatctgg cccagcttgt cgcgccacaa cgtgaccacg
    15121 cagtggctga agtcgaggat caaccgccac ggcgagcgct cctggcgcgg gtagcgcgcg
    15181 cccgtatcgg gaatgcgcgt attgatgaat gccgcgatcg cgtacagcag cgcgatcacc
    15241 ccgatcgccg cttcgccggg ctggtcgatg tcgaagtcga tcagagggaa gtcgaacgac
    15301 gtcagcgacg ccgccacgtg cggcgatacg agcacgccgc cgagcacgaa gccgaggatg
    15361 atcgagccca ccgtcagccc ctcgatccag ccgttggcga ccaccagttt ctccggcggc
    15421 agcagttcgg tcaggatgcc gtacttcgcc ggcgagtaag cggccgcgcc gaggccgacc
    15481 accgcatagg ccgccagcgg gtgcaggccg aacagcatga ccaggcagcc gacgatcttc
    15541 acgccgttgg tgatcagcat cacctggccc ttcggccggg catcggaaaa tgcaccgacg
    15601 aacgcggcca gcatcacgta cgacacgaag aagaacagct tcagcatcgg cgtcatccac
    15661 tccggcgcgt tgatgtcgcg cagcagcgcg atggcggcga tgagcagcgc gttgtcggcc
    15721 agcgacgaga agaactgcgc ggccatgatc acgtagaagc cgcggttcat cggcggaatg
    15781 ggagtggctg cgcgggcaaa ggaacggtcg gaggaggcgg gcgcgcgcgt tatatcacgc
    15841 aggcgatgca ttgccgttgg catatcgccg tgccgccgcc cgttatgctt gccgcatgcc
    15901 gcgaccgatc cgcgccaccg tcgatctcgc cgcgatgcgc cacaacctgg cgcgcgcgcg
    15961 acaggaggcc ggcgcgcgca gggtgtgggc cgtggtcaag gcgaacgcgt acgggcacgg
    16021 catcgagcgc gccgtgcgcg cgttcgccga tgcggacggc ctcgcgctgc tcgacctcga
    16081 cgaggcgcag cgggcacgcg atgctggctg gcgcaagccg atcctgctgc tcgaaggatt
    16141 cttcgagccc ggtgacctcg acgtggtcga cgcgctcgac ctgtcgacgg tcgtgcacca
    16201 gcgcgagcag gtcgagatgc tcgctgccca ccggcccggc cgcgcgatcg aggtctacgt
    16261 aaagctcgac accggcatgc gccggctggg cttcggtgac gatgcgatgg aggtggtcgc
    16321 gcggctgcag tcgctgccgc acgtgcgcgt cgcagcgctc gcgacgcatc tggcgaatgc
    16381 ggatcgatcg gcctccggtc cagccgctgt cgccgatcag ttgcgcgcat tcgcggcggc
    16441 cacgcgtggc tggcagggcg cggtcagcct cgccaattcg gccgcgctgt tcctgcgacc
    16501 cgaggtcggc ggcgattcgg tgcgtccggg catcgcgctg tatggcggtc ggccgcgcgc
    16561 tggacagccg gccacccaac tcggattgcg acccgcgatg cggctgcgtt cgcggctgct
    16621 cgaagtccgg gacctgcagg caggcgacgc ggtcggctac ggcagccgct ggatcgcgca
    16681 gcggccggcg cgcatcggcg tcgtcgcgtg cggctatgcg gacgggtatc cgcgcgtcgc
    16741 gcccgacggc acgccggtgt ggatcgcggg aaagatcgtt cccttggccg gccgcgtgtc
    16801 gatggacatg atcaccgtcg acctggccga tgcgatgcac gcacgggtgg gcgccgaggt
    16861 cgaattgtgg ggcgagcacg tgccgatcga tgacgtggcg gaacaggccg gcaccgtcgg
    16921 ctacgagctg atgtgcgcgc tggcacggcg ggttgccgtc gacgagatcg gctgacgcgt
    16981 ggccaaggtc aagacgcagt tcgtctgcag cgagtgcggc ggtaccagcc cgcgctggca
    17041 gggccagtgc ccgcaatgca acgcatggaa cacgctggtc gaggcgcgag tggaggcggg
    17101 ctcgcgtcgc tacgcgccgc tcgcaccggt ggcgtcggtg cagttgctca gtgagatcga
    17161 ggcgcgcgaa gtcgggcgta cctcgaccgg tatcgaggag ttcgaccgcg tgctcggcgg
    17221 aggtctggtc gagggtggcg tcgtgctgat cggtggcgat ccgggcatcg gcaaatcgac
    17281 gctgctgctg caggcgcttg ccgcgatggc gggcggccag aacgtgctgt atgtcagcgg
    17341 cgaggaatcg gcgggccaga tcgcgctgcg ggcgcggcgc ctcggcgtgg acgggcgcaa
    17401 tgtgcgcttc atggccgaga tcgatctcgg ccgcatcctt ggcgcgctgg aaacgcagcg
    17461 gccgcaggtg gcggtgatcg attcgatcca gacgctgtgc tggagcgagc tcacatcggc
    17521 gccgggatcg gtcgcacagg tgcgcgagtg cgcggcgcag ctcacgcgca tagcgaaggc
    17581 gagcggcatc acgctggtgc tggtagggca cgtgaccaag gagggtgcac tcgccggccc
    17641 gcgcgtgctc gagcacatgg tcgacacggt gctgtacttc gagggcgaca cccattcctc
    17701 gttccgcctg attcgcgc
//
LOCUS       DBNHBY010000092         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1088198, whole genome shotgun sequence.
ACCESSION   DBNHBY010000092 DBNHBY010000000
VERSION     DBNHBY010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1088198"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="AC16HB_02225"
     CDS             complement(<1..676)
                     /locus_tag="AC16HB_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5605635.1"
                     /translation="MKLLILGGTRFLGRHLVDAACATGHEVTLFNRGHTAPELFPHLE
                     HLRGERDGGLDALGDRCWDAVIDTCGYVPRVVRQSAARLSARVARYVFVSSVSVYSDS
                     SCARSAEDAPVAALLDPKSEDVQAHYGALKAACESVVQEVFGRRALIIRPGLIVGPHD
                     PTERFSYWVCRCARGGAMLAPDVPEYPVQFIDARDLARWIIELVQRDIFGIFNAVGPA
                     RPLTLAE"
     gene            complement(881..1816)
                     /locus_tag="AC16HB_02230"
     CDS             complement(881..1816)
                     /locus_tag="AC16HB_02230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5605636.1"
                     /translation="MPFEMRPTTLKVTAAFGAMLALASGCASLDEWQRRSIFQHESSV
                     RFGDREPPAGGVAYDLALVNGDHVHAWFVPALEPGGPTLLYLHGARRNLAGSYGRIEL
                     LRSLGFNVLAIDYRGFGRSSALLPSEQSAIEDTRLAFAELLRREPDPSRRFVYGYSLG
                     GALAIDLAANTDGIAGLIVESTFTSIPDLVRASRWSWVPLLNAIVTQEFDSLSKIARV
                     NEPLLLIHGTADGLIPHAMADRLYAAAINVQPELRRVVKIDGATHWGAAFYGGETYTR
                     ALREFIAAAQTARPAVSAASNLATGAAASTAVDLQ"
     gene            complement(2185..>2443)
                     /locus_tag="AC16HB_02235"
     CDS             complement(2185..>2443)
                     /locus_tag="AC16HB_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5605637.1"
                     /translation="PGGATPPHRHDCDEVVLVLSGSGEVHLDGRVIPFGPDFTLAVPR
                     NVPHQIVNTGYEPLHTVAAFSKSPVGVAFPDGTPLELPWRS"
BASE COUNT          436 a          851 c          801 g          355 t
ORIGIN      
        1 actccgcgag ggtcaacggc cgcgctggcc ccacggcgtt gaagatgccg aagatgtcgc
       61 gttgcacaag ctcgatgatc cagcgcgcca agtcccgcgc gtcgatgaac tgcaccggat
      121 actcgggcac gtccggtgcg agcattgcgc cgccgcgcgc gcaacggcac acccagtaag
      181 aaaagcgttc cgtgggatcg tggggcccga cgatgaggcc cgggcggatg atcagcgccc
      241 gccgcccgaa cacttcttgc acgaccgact cgcacgcggc cttcagcgcg ccgtagtgtg
      301 cttgaacgtc ctcgctcttc gggtcgagaa gagctgcaac cggcgcatcc tcggcgctgc
      361 gggcgcacga agaatcgctg tacacgctga cgctggagac gaacacgtaa cgcgcgacgc
      421 gcgcggacag gcgcgcagcc gactgccgca ccacgcgtgg cacatagcca caagtgtcga
      481 tcacggcatc ccagcagcga tcgcccaatg cgtcgagtcc gccgtcgcgt tcaccgcgca
      541 agtgttccag gtgcggaaac aactccggcg cggtgtggcc ccgattgaac agcgtgactt
      601 catggccggt cgcgcacgcg gcatcgacca gatgccggcc gagaaagcgc gtgccaccca
      661 ggatcagcag tttcatcgcc ttcccgcaat ccggtcgatg gcacgattca gcgcgcgaca
      721 ctcaggacga ccgccgcagg cgggacggca tgcaacgccg acaccgcgag cgcaccgagc
      781 tacagcacct gcgacgtgat cgccattccg ccagccgcgc gctcgagcgg ccaagcatcg
      841 gcgtcgccga gttcaagcgg tcaccgcggc agccgtgcac tcattgcaga tcgaccgccg
      901 tggatgccgc agcgccggtc gccaagttcg aggccgccga cacggcgggc cgagccgtct
      961 gcgcggcagc gatgaattcg cgcagcgcgc gcgtgtaggt ctcgccgccg taaaacgccg
     1021 cgccccagtg tgtagccccg tcgatcttca ccacccgtcg cagttccggt tgaacgttga
     1081 tcgcggccgc gtacagacgg tcggccatcg catgtgggat caagccgtcg gcggtgccgt
     1141 ggatcagcag cagcggctcg ttgacgcgtg cgatcttgct gagcgaatcg aactcctggg
     1201 tgacgatcgc gttcagcaaa ggcacccagc tccagcgcga cgcgcgcacg agatccggaa
     1261 tgctcgtgaa cgtcgattcg acgatcagcc cggcgatgcc atcggtgtta gcggccagat
     1321 cgattgccag cgcgccgccg agactgtaac cgtagacgaa acgccggctc gggtcgggtt
     1381 cccgccgcag cagttcggcg aaggcgagcc gcgtgtcctc gatcgccgac tgctcgctcg
     1441 gcaacagcgc gctcgagcgg ccaaagccgc gatagtcgat cgcgaggacg ttgaagccca
     1501 gcgaccgcag cagttcgata cggccatagc tgcccgccag gttgcggcgc gcgccgtgca
     1561 gatacagcag cgtcggcccg ccgggttcga gcgccggcac gaaccaggcg tgtacgtgat
     1621 cgccgttgac cagagcaagg tcgtacgcga ccccgccggc cggcggctcc cgatcgccga
     1681 agcgcacgct cgactcgtgc tggaagatgc tgcggcgctg ccactcgtcg agactggcgc
     1741 agccgctcgc cagcgccagc atcgctccga acgcggcagt gactttcagc gtggttgggc
     1801 gcatctcgaa cggcatgtct ccaaattacc taacgccttt gcgcgatccc atgcgcaggc
     1861 gtcagatggc tcgcgctttc cgatcggcga tcgcttgcgc aaccgcagca tcaacgcacg
     1921 aggataaatg accgcttacc ggatcccttc atttgtgcag tgcgcgcagc ggatgacgtt
     1981 ccgcgagagc cagtgctgcg ggtggcaggc acccgaatca gtgcctgatg ccgagcgccg
     2041 ccgtgtccgc ggagcggccg atctcgatcc cgccaccgcc gcgcagcgcc cttcggcgct
     2101 cccggtactc gcgtgcggga acgacacgag cagcaccacg gctcggcgcc gatggcgggg
     2161 cgtatccgcg caaccgcacc gcgctcagct gcgccagggc agttcgagcg gcgtgccgtc
     2221 ggggaacgcc acgccgaccg gcgacttcga aaacgccgcc accgtatgga gcggttcata
     2281 gccggtgttg acgatctggt gcggcacgtt gcgcggcacc gcgagcgtga agtccggtcc
     2341 gaacggaatc acgcgaccgt cgagatggac ctcgccgctt cccgacagga ccaagaccac
     2401 ctcgtcacaa tcgtgacggt gcggcggcgt cgcgccgccg ggc
//
LOCUS       DBNHBY010000093         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_546011, whole genome shotgun sequence.
ACCESSION   DBNHBY010000093 DBNHBY010000000
VERSION     DBNHBY010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_546011"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16HB_02240"
     CDS             <1..233
                     /locus_tag="AC16HB_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5605638.1"
                     /translation="PPIFPRAAVKDGITEGAVRVRLAVAADGSVERVETSVSDPRHRV
                     FEPAARAAALTWTFVPGAAGRTHESVIQFRAP"
     gene            complement(235..1386)
                     /locus_tag="AC16HB_02245"
     CDS             complement(235..1386)
                     /locus_tag="AC16HB_02245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR5605639.1"
                     /translation="MTTITCIEDLRVLAQRRVPRMFYEYADSGSWTETTYRANQTDFQ
                     PIKLRQRVAVDMEGRSLRTTMVGQEVAMPVALAPTGLTGMQHADGEILAARAAEQFGV
                     PFTLSMMSICSLEDVAAHTSKPFWFQPYMLRDRAFMQRLIERAQAVKCSALVLTLDLQ
                     VLGQRHKDIRNGLSAPPRLTLRNLLNMMTKPRWCLGMLGTRRRQFGNIVGHVTGVDDM
                     GSLSDWTSKQFDPTLNWDDVAWVRRLWDGPLILKGIMEVEDAQLAARSGAQAIVVSNH
                     GGRQLDGAPSSIAALPAIAEAVGDKIEVWLDSGIRSGQDVLKAVALGARGTMIGRAFL
                     YGLGALGEAGVTRCLEILYKELDLSMAFCGRTKIGDVDRSILVPGTYPN"
     gene            1470..>2431
                     /locus_tag="AC16HB_02250"
     CDS             1470..>2431
                     /locus_tag="AC16HB_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5605640.1"
                     /translation="MSAVPQTAVAEPSRAALEMIDRLIGFPTVSRDSNLGLIEFARDY
                     LAKLGVKPHLVFDAREKKANLFCTLADDPARVQTAGIVLSGHTDTVPVDGQDWASDPF
                     KATHRDGRIHGRGSADMKGFIAIALAWVPRFLTVQKSMPIHLSMTYDEETTFFGVRGL
                     VRDLDERGVKPAGCIIGEPTDMQAIVAHKGKRDYCCRVRGREAHSSLTPTAVNAIEYA
                     AQIIAFIRRLADRNAREEPRDSRFEVPFSTLQTGVIRGGIATNVVPRDCEFMFEMRNI
                     PATKHDAMSGPILAYARDELLPQMKRVAPEAGIDFEMGLDLPAFG"
BASE COUNT          375 a          846 c          826 g          384 t
ORIGIN      
        1 cgccgccgat cttcccgcgc gctgcagtga aggacggcat caccgaaggc gcggtgcgtg
       61 tgcgcctggc ggtcgcggcc gacggcagcg tcgagcgcgt cgaaacgagc gtctccgacc
      121 cgcgccaccg cgttttcgag ccggcggcgc gtgccgcggc gttgacctgg accttcgtgc
      181 ccggcgccgc cggaaggacg cacgagtcgg tgatccagtt ccgcgcgccc tgactcagtt
      241 cggatacgtt cccggcacca ggatcgagcg atcgacgtcg ccgatcttcg tcctgccgca
      301 gaacgccatc gacaggtcga gctccttgta gagaatctcc aggcaccggg tcacgccggc
      361 ttcgccgagc gcgccgagac cgtagaggaa cgcgcggccg atcatcgtgc cgcgcgcgcc
      421 gagcgcgacg gccttcagca cgtcctggcc gctgcggatg ccgctgtcga gccacacttc
      481 gatcttatcg ccgaccgcct ccgcgatcgc gggcagcgcc gcgatcgacg acggcgcgcc
      541 gtcgagctgg cgtccgccgt ggttgctgac gacgatcgcc tgcgcgccgc tgcgggccgc
      601 gagctgtgcg tcctcgacct ccatgatgcc cttgaggatc agcggtccgt cccagagcct
      661 gcgcacccag gccacgtcgt cccagttcag ggtcgggtcg aactgcttgg aggtccagtc
      721 cgacagtgaa cccatgtcgt ccacgccggt cacgtggccg acgatgttgc cgaactgccg
      781 ccgccgcgtg ccgagcatgc cgaggcacca gcgcggcttg gtcatcatgt tcaggaggtt
      841 gcgcagcgtg agcctgggcg gggcggacag gccgttcctg atgtccttgt ggcgctggcc
      901 gagcacctgc aggtcgagcg tgagcacgag cgccgagcac ttgaccgcct gtgcgcgctc
      961 gatcagacgc tgcatgaacg cgcggtcgcg cagcatgtag ggctggaacc agaacggctt
     1021 gctcgtgtgg gccgcgacat cctccagcga gcagatgctc atcatcgaca gcgtgaacgg
     1081 cacgccgaac tgctccgcgg cgcgcgcggc caggatctcg ccgtcggcat gctgcatgcc
     1141 ggtcagcccc gtgggcgcga gcgcgaccgg catcgcgacc tcctgcccga ccatcgtcgt
     1201 gcgcaggctg cggccttcca tgtcgaccgc cacgcgctgg cggagcttga tcggctggaa
     1261 atccgtctga ttggctcggt acgtggtctc ggtccacgac ccgctgtccg cgtactcgta
     1321 gaacatccgc ggcacgcgcc tttgcgccag cacgcgcagg tcctcgatgc aggtgatcgt
     1381 ggtcattgcg gtctccctcc gctgcgcgat ggtaatcgca ctcgatgctc tgcctacaat
     1441 gacgccaccg ggctcaacgc gaggttgcca tgtccgccgt tccgcaaacc gcagtcgccg
     1501 agccatcgcg cgcggcgctg gagatgatcg atcggctgat cggattcccg acggtcagcc
     1561 gcgacagcaa cctggggttg atcgaattcg cgcgcgacta tctggcgaag ctcggggtca
     1621 agccgcacct cgtcttcgat gcgcgcgaga agaaggccaa cctgttctgc acgctggcgg
     1681 acgacccggc gcgcgtgcag accgccggca tcgtgctgtc gggccacacc gacaccgtgc
     1741 cggtcgacgg gcaggactgg gcgagcgatc cgttcaaggc cacgcaccgc gacggccgca
     1801 tccacggccg cggcagcgcc gacatgaagg gcttcatcgc gatcgccttg gcgtgggtgc
     1861 cgcgcttcct gacggtgcag aagtcgatgc cgatccacct gtcgatgacc tacgacgagg
     1921 agaccacgtt cttcggcgtg cgcgggctgg tcagggatct ggacgagcgc ggcgtcaagc
     1981 ccgcgggctg catcatcggc gagcccacgg acatgcaggc gatcgtcgcc cacaagggca
     2041 agcgcgacta ctgctgccgc gtgcgcgggc gcgaggcgca ttcgtcgctg acgccaaccg
     2101 cggtcaacgc gatcgagtac gcggcgcaga tcatcgcgtt catccggcgg ctcgccgatc
     2161 gcaacgcgcg cgaggagccg agggattccc gcttcgaggt gccgttctcg acgctgcaga
     2221 ccggcgtgat ccgcggcggc atcgccacca acgtcgtgcc gcgcgactgc gagttcatgt
     2281 tcgagatgcg caacatcccg gcgacgaagc acgacgcgat gagcgggccg atcctcgcct
     2341 atgcgcgcga cgagttgctg ccgcagatga agcgcgtcgc gcccgaggcg ggcatcgact
     2401 tcgagatggg gctcgacctg cccgcgttcg g
//
LOCUS       DBNHBY010000094         4378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1358557, whole genome shotgun sequence.
ACCESSION   DBNHBY010000094 DBNHBY010000000
VERSION     DBNHBY010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1358557"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="AC16HB_02255"
     CDS             <1..1341
                     /locus_tag="AC16HB_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5605641.1"
                     /translation="RIPRFVVYPSSHYVTPRQTVLRAIEDIKVELKQRVDELVAAGKL
                     VEAQRLEQRTRFDLEMLSELGFCKGIENYTRHLAGSMPGEPPPTLIDYLPADALMIID
                     ESHVTVPQLGGMYRGDRARKETLVEYGFRLPSALDNRPLRFDEFERKMRRCIFVSATP
                     SEFELGRSSQVVEQVVRPTGLVDPRVEVRPARTQVDDVLSEIRLRVEAGDRVLITTLT
                     KRMAEDLSSFLAEHGVKVRYLHSDVDTVERVEIIRDLRAGVFDVLVGINLLREGLDLP
                     EVSLVAILDADKEGFLRSERSLIQTIGRAARHRDGTAIMYADAETDSMRRAIGETNRR
                     REKQEVFNREHGIVPRSVQKEVRELIDGVIDSAPSRAELKAASAVADYEVLSEKQVAR
                     EIKRLEKAMFEHAKNLEFEKAARVRDQLALLRQQVFGAAGSGNVVAFTGNEKAA"
     gene            1545..2033
                     /locus_tag="AC16HB_02260"
     CDS             1545..2033
                     /locus_tag="AC16HB_02260"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR5605642.1"
                     /translation="MTKVLFVCMGNICRSPTAEGVFRKMIRDAGLQDVVQIDSAGTHA
                     YHVGEPPDLRAQQAARRRGYELDGLRARQINQDDFRDYDLILAMDWENLALLQQQCPR
                     AYKHKLHLLMRFAGEHDAATVPDPYYGGPEGFNTVLDYVEDACQGLIDVVRKRATMYA
                     AA"
     gene            2243..2746
                     /gene="iscR"
                     /locus_tag="AC16HB_02265"
     CDS             2243..2746
                     /gene="iscR"
                     /locus_tag="AC16HB_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HIR5605643.1"
                     /translation="MRLTTKGRFAVTAMIDLGLRQHHGPVTLAAISQRQRISLSYLEQ
                     LFGKLRRHGLVESTRGPGGGYTLGRTAADISVADIIYAVDEPLDATQCGGKENCDNDQ
                     RCMTHDLWANLNRVMVDYLDSVSLHDLVEQQKQRLVQQGVNVAQLHDSRRATRRPVTN
                     DKQAAMS"
     gene            2758..3966
                     /locus_tag="AC16HB_02270"
     CDS             2758..3966
                     /locus_tag="AC16HB_02270"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR5605644.1"
                     /translation="MQLPIYLDYSATTPVDPRVVDKMIPYLREHFGNPASRSHAFGWE
                     AEKAVETAREQVAELINADPREIIWTSGATEATNLALKGAAHFYKDKGKHLVTVKTEH
                     KATLDAMRELEREGFEVTYLDVLPNGLLDLEVLKAALRPDTILVSVMFVNNEIGVIQD
                     VAAIGAICRGRGILFHVDAAQATGKVVIDLQALKVDLMSLSAHKTYGPKGVGALYVRR
                     KPRVRLEAQMHGGGHERGLRSGTLPTHQIVGMGEAFRIARLEMATENERVRMLRDRLW
                     KGLSEMEEVHANGDMEHRIPHNLNVSFNFVEGESLIMAIKDIAVSSGSACTSASLEPS
                     YVLRALGRSDELAHSSIRFTVGRFTTQEEIDYAVDLIKRKVAKLRDLSPLWEMHKEGI
                     DLNKVQWAAH"
     gene            3983..>4378
                     /gene="iscU"
                     /locus_tag="AC16HB_02275"
     CDS             3983..>4378
                     /gene="iscU"
                     /locus_tag="AC16HB_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR5605645.1"
                     /translation="MSYSQKVIEHYENPRNVGSFDKSDAEVGTGMVGAPACGDVMKLQ
                     IKVNPQGIIEDARFKTYGCGSAIASSSLVTEWVKGKTLDQAMTIRNTQIAEELALPPV
                     KIHCSILAEDAIKAAINDYRKKHGLDQPAE"
BASE COUNT          850 a         1345 c         1474 g          709 t
ORIGIN      
        1 cggattccac gcttcgtcgt ctatccgtcg tcgcactatg tgacgccacg gcagacggtg
       61 ctgcgcgcga tcgaggacat caaggtcgaa ctgaagcagc gcgtcgacga actggtcgcc
      121 gcgggcaagt tggtcgaggc gcagcgcctc gagcagcgca cgcgcttcga tctggagatg
      181 ctgtccgagc tcgggttctg caagggtatc gagaactaca cgcggcacct ggccggatcg
      241 atgccgggcg agccgccgcc gacactgatc gactatctgc cggcggatgc gctgatgatc
      301 atcgacgagt cgcacgtcac ggttccgcag ctcggcggga tgtatcgcgg tgatcgggcg
      361 cgcaaggaga cgctggtcga gtacggcttc cggctgccgt cggcgctcga caaccggcct
      421 ctgcgcttcg acgagttcga gcgcaagatg cgccgctgca ttttcgtttc ggccactccg
      481 tccgagttcg agctggggcg ctcgtcgcag gtcgtcgagc aggtcgtgcg gccgaccggc
      541 ctggtcgacc cgcgcgtcga agtgcggccc gcgcggaccc aggtcgacga cgtgctgtcg
      601 gagatccggc tgcgcgtgga agccggtgat cgcgtgctga tcacgacgct gaccaagcgc
      661 atggccgagg acctgtcgtc gttcctggcc gagcacggcg tgaaggtgcg ctacctgcac
      721 tcggatgtcg acaccgtcga gcgagtggag atcatccgcg acctgcgcgc cggggtgttc
      781 gacgtgctgg tcggcatcaa tctgttgcgc gagggcctgg acctgccgga ggtatcgctg
      841 gtggcgatcc tcgatgcgga caaggaggga tttctccgct ccgagcgttc gctgatccag
      901 acgatcggcc gcgcggcgcg ccaccgcgac ggcacggcga tcatgtacgc ggacgccgaa
      961 accgactcga tgcggcgagc gatcggcgag acgaatcgcc gccgcgaaaa gcaggaggtc
     1021 ttcaatcgcg agcatggcat cgtgccgcgc agcgtgcaga aggaagtgcg cgagctcatc
     1081 gacggcgtca tcgattccgc cccctcgcgc gctgagctga aggccgccag tgcggtggcg
     1141 gattacgagg ttctgagcga gaaacaggtc gcgcgcgaga tcaagcgcct ggagaaggcc
     1201 atgttcgagc acgcgaagaa cctcgagttc gagaaggcgg cccgcgtgcg cgaccagttg
     1261 gcactgcttc ggcagcaggt attcggcgca gccggaagcg gcaacgtggt cgcgttcacg
     1321 ggcaacgaaa aggcggcatg acgtgtgtat gcggtatggc ttgaacaaag tcccgactcg
     1381 aacaaacgtt cggccggttg aacttcggtt tggagcagcg gtcccttgtg cggaagttcg
     1441 ctctgagggt gttattgggt gaaagcgccg cacggggtat gagccaagcg gcataacgcg
     1501 ggcagcacga acgccggccc gatccagaaa ggcgtgatga ggaaatgacc aaggttctct
     1561 tcgtatgcat gggcaacatc tgccgctcgc ccacggctga aggcgtgttc cgcaagatga
     1621 tccgggacgc cggccttcag gacgtggtcc agatcgattc cgcgggaacg cacgcctacc
     1681 acgtcggcga gcctcccgat ttgcgtgccc agcaggctgc gcgccgtcgc ggctatgagc
     1741 tggacgggtt gcgcgcgcgc cagatcaatc aggatgactt tcgcgactac gacctgatcc
     1801 tggcgatgga ctgggagaac ctcgcgctgc tgcagcagca atgcccgcgc gcctacaaac
     1861 acaagctgca tctgctgatg cgctttgcgg gtgagcacga cgcggcgacg gtgccggatc
     1921 cgtactatgg cggacccgag ggcttcaata cggtcctcga ttacgtggaa gacgcttgtc
     1981 agggcctgat cgacgtcgtg cgcaagcgcg caacgatgta cgcagcggct tgacgattca
     2041 cttgcccgga cgcggcaacg cgcgtccggg ccgtcggttc ggccgccgct ttagcctgcg
     2101 gcactggatt cttgaccaaa accctcgggt tcattagaat tccgaccaac ttggtcggat
     2161 atggcggcgc gggcgcgcca ccgaggaccg ggccaaggtc cgccggccac ttcaatggca
     2221 ggccggccag agagagacgc ctatgagatt gaccaccaaa ggccgctttg cggtcacggc
     2281 aatgattgac ctgggcctgc gacaacatca cggtccggtg acgttggcag cgatcagcca
     2341 gcggcagcgc atttcacttt cgtacctgga gcaactgttc ggcaagctgc gccgccacgg
     2401 actcgtcgag agcacgcgcg gtccgggcgg tggttacacg ctgggccgca cggctgcgga
     2461 tatctccgtc gccgacatca tctatgccgt cgacgaacct cttgacgcaa cccaatgcgg
     2521 cggcaaggaa aactgcgata acgaccagcg ctgcatgacg cacgacctgt gggcgaatct
     2581 gaaccgcgtc atggtggact acctggattc ggtgtcgctg cacgatctgg tcgagcagca
     2641 gaagcagcgc ttggtacagc agggcgtcaa cgtcgcgcag ttgcacgaca gccggcgcgc
     2701 gacacggcgc ccggtgacga acgacaaaca ggcggcgatg agctaggagc agggtcgatg
     2761 cagcttccga tctatctcga ctattcggcc accacgccgg tggatccgcg cgtggtcgac
     2821 aagatgatcc cgtacctgcg cgaacacttc ggcaatccgg cgtcgcgcag ccacgcgttc
     2881 ggctgggaag cggagaaggc ggtcgagacc gcccgcgagc aggtggccga actgatcaac
     2941 gccgatccgc gcgagatcat ctggacctcc ggtgcaaccg aggcaaccaa cctcgcgctc
     3001 aagggcgccg cgcacttcta caaggacaag ggcaagcacc tcgtcaccgt caagaccgag
     3061 cacaaggcga cgctcgacgc gatgcgcgaa ctggagcgcg aaggcttcga ggtcacgtat
     3121 ctcgacgtgc tgccgaatgg cctgctcgac ctcgaggtgc tgaaggccgc gctccgcccc
     3181 gacacgatcc tggtgtcggt gatgttcgtg aacaacgaga tcggcgtgat ccaggatgtc
     3241 gccgcgatcg gcgcgatctg tcgcggccgc ggcatcctgt tccacgtcga tgccgcgcag
     3301 gccaccggca aggtcgtgat cgacctgcag gcgctcaagg tcgacctgat gtcgctgtcg
     3361 gcgcacaaga cttacgggcc caagggcgtc ggcgccctgt acgtcaggcg caagccgcgc
     3421 gtgcggctgg aagcgcagat gcacggcggc ggtcacgagc gcggtctgcg ctcgggcact
     3481 ctgccgacgc accagatcgt cggcatgggc gaggcgttcc gcatcgcgcg gctcgaaatg
     3541 gccacggaga acgagcgcgt ccgcatgctg cgcgaccggt tgtggaaagg actgagcgag
     3601 atggaagagg tccacgccaa cggcgacatg gaacatcgca ttccgcacaa cctgaacgtg
     3661 agcttcaatt tcgtggaagg ggaatcgctg atcatggcga tcaaggacat cgccgtgtcg
     3721 tcggggtcgg cgtgcacgtc ggcgagcctg gaaccgtcgt acgtactgcg cgcgctcggc
     3781 cgcagcgacg agctggcgca cagctcgatc cggttcaccg tcgggcgctt caccacgcag
     3841 gaagagatcg actacgcggt ggatctcatc aagcgcaagg tcgccaagct gcgcgacctg
     3901 tcgccgctgt gggagatgca caaggaaggc atcgatctga acaaagtgca atgggccgca
     3961 cactagtcat agaggaaacg tcatgtcgta cagccagaag gtcatcgagc attacgagaa
     4021 cccgcgcaac gtcggctcgt tcgacaagag cgacgccgaa gtcggcaccg gcatggtcgg
     4081 cgcgccggcg tgcggtgacg tgatgaagct gcagatcaag gtgaatccgc agggaatcat
     4141 cgaggatgca cgcttcaaga cctacgggtg cggctcggcg atcgcctcgt cttcgctcgt
     4201 gaccgaatgg gtcaagggca agacgctcga ccaggcgatg acgatccgca acacgcagat
     4261 cgccgaggaa ctggcgctgc cgccggtcaa gattcactgc tcgatcctgg ccgaggacgc
     4321 gatcaaggcg gcgatcaacg attaccgcaa gaagcacggt ctcgaccagc ccgcggaa
//
LOCUS       DBNHBY010000095         2026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_275830, whole genome shotgun sequence.
ACCESSION   DBNHBY010000095 DBNHBY010000000
VERSION     DBNHBY010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_275830"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16HB_02280"
     CDS             complement(<1..194)
                     /locus_tag="AC16HB_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605646.1"
                     /translation="MQTRSSDPVGTAPPSIALPRLSVPEAERLLRGGLTLAAEMFGAA
                     FPAMCRPAADALIARLDSDPG"
     gene            complement(316..1092)
                     /locus_tag="AC16HB_02285"
     CDS             complement(316..1092)
                     /locus_tag="AC16HB_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5605647.1"
                     /translation="MGRTRRPARAASPPPRVTISEQAGVRYLHFGTEWIQGAMRIARP
                     FALELDYQKQMMAPLLFLPEPRRIVQLGLGAAALAKACWRALPDAEIRVVEISAEVVD
                     SARQWFKLPDSDRLAITQEDAESFVAHPRRRGCADWLQIDLYDAAARGPVCDSAEFYR
                     HCRALLAAPGVAAINLFGRSFEPSFARIAAAFDDRAIVLPEAEAGNRIVLAFTGAPLV
                     LPFAQLYERAGDIERRFGWPARRWVSGVKSGNGFERDFIL"
     gene            complement(1092..1583)
                     /locus_tag="AC16HB_02290"
     CDS             complement(1092..1583)
                     /locus_tag="AC16HB_02290"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR5605648.1"
                     /translation="MPAPRRLKGFPPVLDARVRRLILGSFPSEASLAAGQYYAHPRNQ
                     FWRLLGALIGEPLAELPYEQRLQRVLAHRIGIWDVLDACEREGSLDSSIRRHRPNDFT
                     RLKRLAPSLQRVIFNGGTAGRFAREFADAGFETRIAPSTSPAHAGRSFAQKLALWRTA
                     LED"
     gene            complement(1583..>2026)
                     /locus_tag="AC16HB_02295"
     CDS             complement(1583..>2026)
                     /locus_tag="AC16HB_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702271.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR5605649.1"
                     /translation="SVAKGDLDLGDLADAQEKEEQAKVADEYKELVARVKAALADRAQ
                     DVRVTLRLIDSPACIVADRDAMSQHLQRLLKAAGQKAPEVRPILELNPNHPLVQRLKY
                     ESDDKLADWAALLLDQAVLADGGSLDDPAAFVKRMNQMLLDAAAK"
BASE COUNT          299 a          740 c          695 g          292 t
ORIGIN      
        1 ccagggtcgg agtccagccg cgctatcagg gcgtccgccg caggccggca cattgcgggg
       61 aacgcggcgc cgaacatctc ggccgccagc gtcaggccgc cgcgcagcaa acgctcggcc
      121 tcaggtaccg aaagccgcgg cagcgcaatg ctcggaggcg cggtgccgac cggatcggac
      181 gaacgtgtct gcatggggcg gagattatgc gcgagtgagg aatccctcac aaacccgggt
      241 cagactatcc gtcggcccgg cactgtcaag cacgcgatcc tgcatcagcc gctcggatcg
      301 cgggcgccgg tccgctcaaa ggatgaagtc gcgctcgaag ccgttacctg acttgacgcc
      361 cgacacccag cgccgcgccg gccagccgaa tcggcgttcg atgtcgccgg cgcgctcata
      421 cagctgcgcg aacggcagca ccagcggcgc gccggtgaat gcgagcacga tgcggttgcc
      481 ggcctcggct tcgggcagca cgatcgcgcg atcgtcgaac gcggcggcga tgcgcgcgaa
      541 gctcggctcg aagctgcgcc cgaacaggtt gatcgccgcc acgccgggcg cagccagcag
      601 agcgcggcag tggcggtaga actcggcgct gtcgcagacc ggcccgcgcg ccgcggcgtc
      661 gtacaggtcg atctgcagcc aatcggcgca gccgcgccgg cgcgggtgcg cgacgaacga
      721 ctcggcgtcc tcctgcgtga tcgccagccg gtcgctgtcg ggcagcttga accactggcg
      781 cgcgctgtcg acgacctcgg cgctgatctc gaccacccgg atctccgcgt cgggcagcgc
      841 gcgccagcat gccttggcga gcgctgccgc gccgagtccg agctgcacga tgcggcgcgg
      901 ttcgggcagg aacagcagcg gcgccatcat ctgcttctgg taatcgagct cgagcgcgaa
      961 cgggcgcgcg atccgcatcg ccccttggat ccactcggtg ccgaagtgca ggtagcgcac
     1021 gccggcctgc tccgagatgg tcacccgcgg cggcggcgat gccgcgcgcg ccggacgccg
     1081 ggtgcgcccc atcagtcttc cagcgccgtg cgccacagcg cgagcttctg cgcgaagctg
     1141 cgccccgcgt gcgccgggct cgtcgacggc gcgatacgcg tctcgaagcc tgcgtcggcg
     1201 aactcgcgcg cgaatcggcc tgcggtgcca ccgttgaaga tcacgcgctg cagactcggc
     1261 gccaaccgct tcagccgcgt gaagtcgttc ggccggtggc ggcggatgct gctgtcgaga
     1321 ctgccctccc gctcgcacgc gtcgagcacg tcccagatgc cgatgcggtg cgcgagcacc
     1381 cgctgcaggc gctgctcgta cggcagctcc gccagcggct cgccgatcag cgcgccgagc
     1441 aggcgccaga actggttgcg cggatgcgcg tagtactgcc ccgcggcgag cgatgcctcg
     1501 ctcggaaaac tgccgagaat cagccgccgc acgcgcgcgt cgagcacggg cggaaagccc
     1561 ttgagccgcc tgggcgccgg cactacttcg ccgccgcgtc cagcagcatc tggttcatgc
     1621 gcttgacgaa cgcggccggg tcgtcgagcg atccgccgtc ggcgagcact gcctggtcga
     1681 gcagcagcgc ggcccagtcg gcgagcttgt cgtccgactc gtacttgaga cgctgcacca
     1741 gcggatgatt cgggttcagt tccaggatcg gcctgacctc cggcgctttc tgcccggccg
     1801 ccttcagcag ccgctgcagg tgctggctca tcgcgtcgcg gtcggccacg atgcacgccg
     1861 gcgagtcgat gagccgcagc gtcacgcgca cgtcctgcgc ccggtccgcc agcgccgcct
     1921 tcacgcgcgc gacgagctcc ttgtactcgt ccgcgacctt ggcctgttcc tccttttcct
     1981 gcgcgtcggc gagatccccc agatcgagat ctcccttcgc caccga
//
LOCUS       DBNHBY010000096         8674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_411105, whole genome shotgun sequence.
ACCESSION   DBNHBY010000096 DBNHBY010000000
VERSION     DBNHBY010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_411105"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2055)
                     /gene="nuoL"
                     /locus_tag="AC16HB_02300"
     CDS             complement(172..2055)
                     /gene="nuoL"
                     /locus_tag="AC16HB_02300"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR5605650.1"
                     /translation="MGALTLIVLLPLAGFLLNGLFGNRFGHPFVNVVGCGLPIAAFAI
                     ALKTTVDFLAGGGVPIVEPFFTWAAIGTNVFDISFYFDRLTVVMTLIVTGVGSLIHIY
                     SIGYMHKDDSYARFFAYLNLFLFFMLMLVLGRSLLVLFVGWEGVGLASYLLIGFWFED
                     PLNAAAGKKAFIANRIGDAGFLLGMFLLYTALGTLEMDRINAAFLAPNASAALLGLAT
                     AVGVFLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMTARMSGIYM
                     HSEAASNLIAVIGAATAFLAATIAIAQTDIKKVLAYSTVSQLGFMFVAVGVGAYGVAV
                     FHLYTHAFFKACLFLGAGSVIHAMSGEQDIRKMGALARRIPLTSVTFIIATAAIAGIP
                     FLSGFFSKDEILLYALVSPLGSQVLFGVMAATALMTAFYMFRLLWLVFFGHSRVEPEV
                     DHHIHESPRSMTWVLVILAFLSVVGGYLSIPHFLAPQLPLPAVEEAAHHYHWTVVGVS
                     VALGLIGLAGAWFVYGGAGTLGERLLATFAPLHKLLYAKYYIDELYELLLGRPLLWIS
                     DRLFLRVGDRFVIDGTLNGLAALAQRAAGRLQLVQAGSLHLYALFVLVGIVAALVWSW
                     RYV"
     gene            complement(2058..2360)
                     /gene="nuoK"
                     /locus_tag="AC16HB_02305"
     CDS             complement(2058..2360)
                     /gene="nuoK"
                     /locus_tag="AC16HB_02305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR5605651.1"
                     /translation="MDKVHMLLGLSFALFALGLTGVIVRRNLLVMLMCMELMLNGVNL
                     SLVTFAHQMGAAAGAVLVFLVFVVATAEIAIAIPIVLLLVRRKRTLDAAAYADLKG"
     gene            complement(2353..2877)
                     /locus_tag="AC16HB_02310"
     CDS             complement(2353..2877)
                     /locus_tag="AC16HB_02310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR5605652.1"
                     /translation="MIDALLANLDTLLLAVFALGALIGAALMLVLKQPMRVALALIST
                     MVFLGGIYGLLGVHFIAAFQVLIYVGAVMVFMVYVIMLLDPRDALISRYSRWLVPGVI
                     GFALLFGALAIGIWQALPAAALRTSATGFGLAQFATAFLNEYWLHFELTSVLLVAAVV
                     AALAVIKVGGKPRG"
     gene            complement(2874..3455)
                     /locus_tag="AC16HB_02315"
     CDS             complement(2874..3455)
                     /locus_tag="AC16HB_02315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR5605653.1"
                     /translation="MARKPFVRKRYWNEPEMGWYERAYLFEILRGLYITSVVFLRNMG
                     KWITGRKGAITTYYPEERRWDYARNNRGKHVLTQRPDGSPQCIACNMCATVCPAKVIE
                     IDAALDPADPAHPKYPSRFEIDYSRCVFCGLCVEACPEDAIRMVPDVPGFPSGDVHRM
                     WVGKEELLTWRPQQDVAKPYPPKGAATQARGHG"
     gene            complement(3458..4594)
                     /locus_tag="AC16HB_02320"
     CDS             complement(3458..4594)
                     /locus_tag="AC16HB_02320"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR5605654.1"
                     /translation="MIQDLVIYVIFITYAISVLMGFGTILTWVERKQAAVMSDRIGAN
                     RAYLRIPFTQVKLVWWGLFHGMADGLKMLLKEDFKPQTYDWYAYAVAPWVVFTPVLLV
                     FAVVPFGGMLNPGQLFPALADWFGGKTYPMQIATLDAGLLIVFAFGGMTIIGSMLAGW
                     SSSNKFSLLGALRAGSQMISYELIMGLTVLGLIVIYGTVDLDNIVRQQSGTVGWLPAW
                     GVVYQPFAATLFMAAAIAENKRIPFDLPEAESELIAGFYTEYSAMKMGLFMFAEFIEI
                     AIIAALFTTLFLGGYNLPYMNDKGFAFPGGTVIEMSHGAVVVTQLVVFLAKVFVISSF
                     QILIRWTLPRFRYDQVLRFAWKFMFPLALANLTVTAVWLWVVKG"
     gene            complement(4591..6033)
                     /locus_tag="AC16HB_02325"
     CDS             complement(4591..6033)
                     /locus_tag="AC16HB_02325"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR5605655.1"
                     /translation="MPDFFINGQPVHAERDQTVMQAALAAGFFIPYFCWHPNLSVAGN
                     CRICVVEVEDPKGGNWIEIACNMPVAEGMRVLTDSEGVRARRKETLQLILLNHPVDCG
                     ICDKAGECTLQDYHYTYNGERSVSVDAKVPATKFHPLSERVIIDNERCIFCSRCVRFT
                     HEVSKSCSLGIQSRGDHSLVRASEDGAFATDPYSDNVIDICPVGALLSKNFLYKSRVW
                     YLKATPSVCPGCERGCSVDVWHRKPEWKLNALDPKQNATIARVTPRENAAVNGPWICN
                     KGRDLAQLFERPRAEQALLKGKPVESSTAIESARRLIEGASKPVALVSSWGSNEELDA
                     FKRALGGRCTSFVKTDWQPQPGERVEDDVLIRPDKNPNGKRARELFGATAVQFPAGTD
                     LVLVWGEGCNFAELPRGVKIVYLNSYLAPENGHADVFIPISIQTERAGSYTNFAGTVS
                     RFEACFAKKPGVVHAEALFARLGAKEKVTA"
     gene            complement(6026..7330)
                     /gene="nuoF"
                     /locus_tag="AC16HB_02330"
     CDS             complement(6026..7330)
                     /gene="nuoF"
                     /locus_tag="AC16HB_02330"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR5605656.1"
                     /translation="MTGAKFIMNFPVTATSHTLADYRARGGYQTIRKALTSMRPEDVT
                     KEVTASGLLGHGGAAFPVGRKWAVIKPNDGQPHHLCANADEGEPGTFKDRWVLEHAPH
                     LLIESMLITSYAIGCRHSFVYIRGEFDLPYRRLQAAVDEAYAAGLLGVPLFGTDFALD
                     LFVYRGAGSYVCGEASGLLTSIEGKKGYPRNRPPRLTVRGLYQRPTVVNNVETLANIT
                     GIIANGAEAFKKTGTAKSPGTQLVSISGHVVKPGVYEVEYGYPLAKFLYEDCGGMLGG
                     KKLKALIPGGISTKVLKEEDIAPLTYDHASFDKAGSSLGSGGMIAIADGTCMVRLLQV
                     MLRFYHHESCGQCTPCREGMGWMHRIIDRIVEGQGVPQDIDRLIAISNANDGTTICGM
                     GDAAGYATVGIIAKFRNEFEYFIANKRSQCNGALEVEGTWHA"
     gene            complement(7327..7803)
                     /gene="nuoE"
                     /locus_tag="AC16HB_02335"
     CDS             complement(7327..7803)
                     /gene="nuoE"
                     /locus_tag="AC16HB_02335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5605657.1"
                     /translation="MSSVVTKLTPYFEQLKHRLPEGFESSLVLPCLRRIQEERGYVAD
                     EDVAALTAYIGVPRMQIEQVLSYYTQFRRKPIGRWHLQVCHNVTCSMRGAERALDMLQ
                     KRLHIKPGQTTADGRFTLSTVECLGSCGTAPVVMVNDTYHESLNGMKLDALLESLK"
     gene            complement(7800..>8674)
                     /locus_tag="AC16HB_02340"
     CDS             complement(7800..>8674)
                     /locus_tag="AC16HB_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR5605658.1"
                     /translation="LAEYSRIADHMTCTAAALMELGAMTAFLYLVTIRDAIYEHLADL
                     TGARVTYTYGRIGGLARDLPDGWLKRLEEILEQSDDFLGRVHGLVDRNRIFIDRMRNV
                     GCLSSTTAVNWGFTGPVLRSTGVPRDLRKDTPYLAYAELDFEIPVGIKGDNYDRYFVR
                     MREIDESKHMIRQLVDRLEPGPINVDDRRCMLPEKEGVYREIEALINHFKLIMEGPQV
                     PAGDIYVAHEAANGELGFYLVSNGSGTPYKMHVRAPSFVHLGGAHLMLDGGQLADIIP
                     TFGQINMIGGECDR"
BASE COUNT         1537 a         2912 c         2864 g         1361 t
ORIGIN      
        1 ccggcgatcg ccgggtcgaa gcgcgcatac aggatggccg tcaggaagaa ctgcgcgacc
       61 atcgcgccga acgacagcgc gcgtacgcgg tcctcgtgcc cctccggcgt catcgccacg
      121 agcgcgatgc cgaccagcgg caggtacagc acaacgttca gcaggaaggc ctcagacata
      181 gcgccagctc cacacgaggg cggcgacgat gccgaccagc acgaacagtg catacaggtg
      241 caggctgccc gcctgcacca actgcagccg ccccgcggcg cgctgcgcca gcgccgccag
      301 gccgttgagc gtgccgtcga tcacgaaacg gtcgccgacg cgcaggaaga ggcggtccga
      361 gatccacagc agcggccggc cgagcagcag ttcgtacagc tcgtcgatgt agtacttcgc
      421 gtacagcagc ttgtgcagcg gcgcgaaggt cgccagcagg cgttcgccga gcgtgccggc
      481 gccgccgtag acgaaccacg cgccggccag cccgatcaag ccgagcgcga ccgacacgcc
      541 gaccaccgtc cagtggtagt ggtgcgccgc ttcttcgacg gccggcagcg gcaactgcgg
      601 cgcgaggaag tgcggaatcg acagatagcc gccgaccacc gacaggaagg cgaggatcac
      661 cagcacccag gtcatcgacc gtggcgactc gtggatgtgg tggtcgacct cgggctcgac
      721 gcgcgaatga ccgaagaaca ccagccacag caaccggaac atgtagaacg cggtcatcag
      781 cgcggtcgcc gccatcacgc cgaacagcac ttgcgagccc agcgggctga ccagcgcata
      841 cagcaggatc tcgtccttcg agaagaagcc cgacaggaac gggatgccgg cgatcgcggc
      901 ggtggcgatg atgaaggtca ccgacgtgag cggaatccgg cgcgccagcg cgcccatctt
      961 gcggatgtcc tgctcacccg acatcgcgtg gatcacggat cccgcgccga ggaacaggca
     1021 cgccttgaag aacgcgtgcg tgtaaaggtg gaacaccgcc acgccgtacg cgccgacgcc
     1081 gaccgcgacg aacatgaagc ccagttgcga cacggtcgag tacgcgagca ccttcttgat
     1141 gtcggtctgg gcgatcgcga tcgtcgccgc gaggaacgcg gtggccgcgc cgatcaccgc
     1201 gatcaggttc gacgccgcct cgctgtgcat gtagatgccc gacatgcgcg cggtcatgta
     1261 cacgcccgcg gtcaccatcg ttgccgcgtg gatcagcgcg gacaccggcg tcgggcccgc
     1321 catcgcgtcg ggcaaccata cgtacagcgg gatctgcgcc gacttgccgg tggcgccgat
     1381 gaacaggaac acgcccacgg cggtcgccag cccaagcagc gcggccgagg cgttcggcgc
     1441 caggaacgcg gcgttgatgc ggtccatctc gagcgtgccc agcgccgtgt acagcaggaa
     1501 catgccgagc aggaagccgg cgtcgccgat gcggttggcg atgaacgcct tcttgcccgc
     1561 cgccgcgttc agcgggtcct cgaaccagaa gccgatcagc aggtacgacg cgagtccgac
     1621 gccttcccag ccgacgaaca gcaccagcag cgagcggccc agcaccagca tcagcatgaa
     1681 gaacaggaac aggttcaggt acgcgaagaa gcgcgcgtag ctgtcgtcct tgtgcatgta
     1741 gccgatcgag tagatgtgga tcagcgagcc gacgccggtg acgatcagcg tcatcacgac
     1801 ggtcaggcgg tcgaagtaga acgagatgtc gaacacgttg gtgccgatcg cggcccaggt
     1861 gaagaacggc tcgacgatcg gcacgccgcc gccggcgaga aaatcgaccg tcgtcttgag
     1921 cgcgatcgcg aacgcggcga tcggcagccc gcagccgacc acgttgacga acggatggcc
     1981 gaagcggttg ccgaacaggc cgttcagcag gaagccggcg agcggcagca ggacgatcag
     2041 cgtgagcgcg cccatggtca tcccttcagg tccgcgtacg ccgccgcatc gagcgtgcgc
     2101 ttgcgccgca ccagcagcag cacgatcggg atcgcgatcg cgatctcggc ggtcgcgacc
     2161 acgaacacga ggaacaccag caccgcgccg gcggccgcgc ccatctggtg cgcgaacgtg
     2221 accaggctca ggttcacgcc gttcagcatc agttccatgc acatcagcat cacgagcagg
     2281 ttgcggcgga cgatcacgcc ggtcagcccg agcgcgaaca gggcgaacga caggccgagc
     2341 agcatgtgca ccttatccac ggggcttgcc tccgaccttg atcaccgcca gcgccgcgac
     2401 gaccgcggcc accagcagca ccgacgtcag ttcgaaatgc agccagtact cgttgaggaa
     2461 cgccgtggcg aactgcgcca gcccgaaccc ggtggcgctc gtgcgcagcg ccgcggccgg
     2521 cagcgcctgc cagatgccga tggccagcgc gccgaacagc agcgcaaagc cgatcacgcc
     2581 cggcaccagc cagcgcgagt aacggctgat cagcgcgtcg cgcgggtcga gcagcatgat
     2641 cacgtacacc atgaacacca tcaccgcgcc gacgtagatc agcacctgga acgcggcgat
     2701 gaagtgcacg cccagcagac cgtagattcc gcccaggaac accatggtcg agatcagcgc
     2761 gagcgcgacg cgcatcggct gcttcagcac cagcatcagc gccgcgccga tcaaggcgcc
     2821 gagcgcgaac accgcgagca gcagcgtgtc gaggttggcg agcagggcgt cgatcatccg
     2881 tggcctcgcg cttgagtggc ggcgcccttc ggcgggtacg gcttcgccac gtcctgctgc
     2941 ggccgccagg tcagcagttc ttccttgccg acccacatgc ggtgcacgtc gcccgacggg
     3001 aaacccggta cgtccggcac catgcggatc gcatcctccg ggcacgcttc gacgcacagg
     3061 ccgcagaaca cgcagcgcga atagtcgatc tcgaagcggg acgggtactt cgggtgcgcc
     3121 ggatcggcgg gatcgagcgc ggcatcgatc tcgatcacct tggccgggca cacggtcgcg
     3181 cacatgttgc aggcgatgca ctgcggactg ccgtcggggc gctgcgtcag aacgtgcttg
     3241 ccgcggttgt tgcgcgcgta atcccagcgc cgctcctccg ggtagtacgt ggtgatcgcg
     3301 cccttgcgcc cggtgatcca cttgcccatg ttgcgcagga acacgacgct cgtgatgtag
     3361 agcccgcgca ggatctcgaa caggtacgcg cgctcgtacc agcccatctc gggctcgttc
     3421 cagtaccgct tgcggacgaa gggcttgcgc gccatggtca tcccttcacc acccacagcc
     3481 acaccgcggt gacggtgagg ttcgccagcg cgagcgggaa catgaatttc cacgcgaagc
     3541 gcagcacctg gtcgtagcgg aagcgcggca gcgtccagcg gatcaggatc tggaagctcg
     3601 aaatgacgaa caccttggcc aggaacacca cgagctgcgt gacgacgacc gcgccgtggc
     3661 tcatctcgat cacggtgccg cccgggaacg cgaagccctt gtcgttcatg tacggcaggt
     3721 tgtagccgcc gaggaacagc gtcgtgaaca gcgccgcgat gatcgcgatc tcgatgaact
     3781 cggcgaacat gaagaggccc atcttcatcg ccgagtactc ggtgtagaag ccggcgatca
     3841 gttccgactc cgcttccggc aggtcgaacg ggatgcgctt gttctcggcg atcgccgcgg
     3901 ccatgaagag cgtggccgcg aacggctggt agacgacacc ccacgccggc agccagccca
     3961 ccgtgccgga ctgctggcgc acgatgttgt cgagatcgac cgtgccgtag atcacgatca
     4021 ggccgagcac cgtcagcccc atgatcagct cgtacgagat catctgcgag cccgcgcgca
     4081 gcgcgcccag cagcgagaac ttgttcgacg acgaccagcc cgcgagcatc gagccgatga
     4141 tcgtcatgcc gccgaacgcg aacacgatca gcaggccggc gtcgagcgtg gcgatctgca
     4201 tcgggtaggt cttgcccccg aaccagtcgg cgagcgccgg gaacagttgc cccgggttca
     4261 gcatgccgcc gaacggcacc accgcgaaca ccagcagcac cggcgtgaac acgacccacg
     4321 gcgccaccgc gtacgcgtac cagtcgtagg tctgcggctt gaagtcctct ttcagcagca
     4381 tcttcagccc gtcggccatg ccgtggaaca ggccccacca gacgagcttc acctgcgtga
     4441 acgggatgcg caggtacgcg cggttggcgc cgatgcggtc cgacatcaca gcggcctgct
     4501 tgcgctcgac ccaggtgaga atggtgccga accccatcag cacgctgatc gcgtacgtga
     4561 tgaagatgac gtagatgacc aggtcctgga tcatgcggtc accttctcct tcgcgccgag
     4621 ccgcgcgaac agcgcctcgg cgtgcaccac gcccggcttc ttcgcgaagc acgcctcgaa
     4681 gcggctcacg gtgccggcga agttcgtgta cgagccggcg cgctcggtct ggatgctgat
     4741 cgggatgaac acgtcggcgt gtccgttctc cggcgccagg tacgaattca ggtagacgat
     4801 cttcacgccg cgcggcagct cggcgaagtt gcagccctcg ccccacacca gcacgaggtc
     4861 ggtgccggcc gggaactgca ctgcggtcgc gccgaacagc tcgcgcgccc gcttgccgtt
     4921 cggattcttg tcgggccgga tcagcacgtc gtcctcgacg cgctcgcccg gttgcggctg
     4981 ccagtcggtc ttcacgaagc tcgtgcagcg gccgccgagc gcgcgcttga aggcatcgag
     5041 ttcctcgttc gagccccagc tcgacacgag cgcgaccggt ttgctcgcgc cctcgatcag
     5101 ccggcgcgcg ctttcgatcg cggtactcga ctcgaccggt ttgcctttca gcagcgcctg
     5161 ctcggcgcgc gggcgctcga agagctgcgc caggtcgcgg cccttgttgc agatccacgg
     5221 tccgttgacg gccgcatttt cgcgcggcgt cacgcgcgcg atcgtcgcgt tctgtttcgg
     5281 gtcgagtgca ttgagcttcc actccggctt gcggtgccag acgtcgacac tgcaaccgcg
     5341 ttcgcagccc gggcacacgg aaggcgtggc cttcaggtac cagacgcgcg acttgtagag
     5401 gaagttcttc gacagcagcg ccccgaccgg gcagatgtcg atcacgttgt ccgaatacgg
     5461 atcggtggcg aacgcgccgt cctcgcttgc cctcacgagc gaatgatcgc cgcggctctg
     5521 gatgccgagc gagcacgact tcgacacctc gtgcgtgaag cgcacgcagc gcgagcagaa
     5581 gatgcagcgc tcgttgtcga tgatcacgcg ctccgacagc ggatggaact tggtcgccgg
     5641 caccttggca tcgaccgaca ccgagcgttc gccgttgtag gtgtagtggt agtcctgcag
     5701 cgtgcactca cccgccttgt cgcagatgcc gcagtcgacc ggatggttca gcaggatcag
     5761 ctgcagcgtc tccttgcgcc gcgcgcgcac gccttccgag tcggtcagca cacgcatgcc
     5821 ttcggcgacc ggcatgttgc aggcgatctc gatccagttg ccgcccttcg ggtcctcgac
     5881 ctcgaccacg cagatgcggc agttgccggc gaccgacagg ttcgggtgcc agcagaagta
     5941 cggaatgaag aagccggcgg cgagcgccgc ctgcatcacg gtctggtcgc gctcggcgtg
     6001 gaccggttgc ccgttgatga agaagtcagg catgccaggt cccttccacc tcgagcgcgc
     6061 cgttgcactg cgagcgcttg ttggcgatga agtactcgaa ctcgttgcgg aacttggcga
     6121 tgatcccgac cgtcgcatag ccggccgcgt cgcccatgcc gcagatcgtc gtgccgtcgt
     6181 tggcgttgga gatcgcgatc aggcggtcga tgtcctgcgg cacgccctgg ccctcgacga
     6241 tgcggtcgat gatccggtgc atccagccca tgccctcgcg gcacggcgtg cactggccgc
     6301 acgattcgtg gtggtagaag cgcagcatca cctgcagcag ccgcaccatg caggtgccgt
     6361 cggcgatcgc gatcatgccg cccgatccga gcgatgagcc cgccttgtcg aacgacgcat
     6421 ggtcgtacgt gagcggcgcg atgtcttctt ccttcagcac cttggtcgag atgccgcccg
     6481 ggatcagcgc cttcagtttc ttcccgccga gcatgccgcc gcagtcctcg tagaggaact
     6541 tggcgagcgg atagccgtac tcgacctcgt acacgccggg cttcaccacg tggcccgaga
     6601 tcgacacgag ctgcgtgccg gggctcttgg cggtgccggt cttcttgaac gcttcggcgc
     6661 cgttggcgat gatgccggtg atgttggcga gcgtctcgac gttgttcacc accgtcggcc
     6721 gctgatacag accccggacc gtcaggcgcg gcgggcggtt gcgcggatag cccttcttgc
     6781 cctcgatcga ggtcagcagc cccgacgcct cgccgcacac gtacgagccg gcgccgcgat
     6841 agacgaacag atccagcgcg aagtcggtgc cgaagagcgg cacgccgagc aggccggctg
     6901 cgtaggcttc gtcgacggca gcctgcagcc gccgatacgg caggtcgaac tcgccgcgga
     6961 tatagacgaa gctgtgccgg cagccgatcg cgtaggaggt gatcagcatc gactcgatca
     7021 gcaggtgcgg cgcatgctcg agaacccagc ggtccttgaa cgtgccgggc tcgccctcgt
     7081 cggcgttcgc gcacaggtgg tgcggctggc cgtcgttggg cttgatcacc gcccacttgc
     7141 ggccgaccgg aaacgccgcg ccgccgtggc cgagcagccc ggacgcggtg acttccttgg
     7201 tcacgtcctc cggccgcatc gatgtcagcg ctttccggat cgtctggtag ccgccgcgcg
     7261 cgcgatagtc ggcgagcgtg tgcgacgtcg ccgtcaccgg gaagttcatg atgaatttcg
     7321 cgccggtcat ttgagcgact ccagcagcgc gtcgagcttc atgccgttca ggctctcgtg
     7381 ataggtgtcg ttgaccatca cgaccggcgc ggtgccgcac gagcccaggc attcgacggt
     7441 ggacagcgtg aagcggccat cggcggtggt ctgccccggc ttgatgtgca ggcgcttctg
     7501 cagcatgtcg agcgcgcgct cggcgccgcg catcgagcag gtcacgttgt ggcacacctg
     7561 caggtgccag cggccgatcg gcttgcggcg gaactgcgtg tagtacgaca gcacctgctc
     7621 gatctgcatg cgcggcacgc cgatgtacgc cgtcagcgcg gcgacgtcct cgtcggcgac
     7681 gtagcccctc tcctcctgga tgcggcgcaa gcacggcagc accagcgatg attcgaaccc
     7741 ttccggcagc cgatgcttga gctgctcgaa gtacggcgtg agcttcgtaa cgactgagct
     7801 catcggtcgc attccccgcc gatcatgttg atctggccga acgtggggat gatgtcggca
     7861 agctgtccgc cgtcgagcat caggtgcgcg ccgcccaggt gcacgaagct cggcgcgcgc
     7921 acgtgcatct tgtacggcgt gccgctgccg ttgctgacga gatagaagcc cagttcgccg
     7981 ttggccgcct cgtgcgccac gtagatgtcg ccggcgggca cctgcgggcc ttccatgatc
     8041 agcttgaagt ggttgatcag cgcctcgatc tcgcggtaga cgccttcctt ctcgggcagc
     8101 atgcagcggc gatcgtcgac gttgatcggg ccgggctcga gcctgtcgac gagctggcgg
     8161 atcatgtgct tcgattcgtc gatctcgcgc atccgcacga agtagcggtc gtagttgtcg
     8221 cccttgatgc cgaccgggat ctcgaaatcc agctcggcgt aggcgagata gggcgtgtcc
     8281 ttgcgcaagt cgcgcggcac cccggtggaa cgcaggaccg gtccggtgaa gccccagttg
     8341 accgcggtcg tgctcgacag gcagccgacg ttgcgcatgc ggtcgatgaa gatgcggttg
     8401 cggtcgacca gcccgtgcac gcggccgagg aagtcgtcgg actgctcgag aatctcctcc
     8461 aggcgcttca gccagccgtc cggcaggtcg cgcgcgagcc cgccgatgcg gccgtacgta
     8521 taggtgacgc gcgcgccggt caggtcggcg aggtgctcgt agatcgcgtc gcggatcgtg
     8581 accaggtaca ggaacgcggt catcgccccg agctccatca gcgcggcggc ggtgcaggtc
     8641 atgtggtcgg cgatgcgcga gtattccgcc agca
//
LOCUS       DBNHBY010000097         3716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1561812, whole genome shotgun sequence.
ACCESSION   DBNHBY010000097 DBNHBY010000000
VERSION     DBNHBY010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1561812"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="AC16HB_02345"
     CDS             complement(<1..1824)
                     /locus_tag="AC16HB_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5605659.1"
                     /translation="MAGIDRQRWARISPLLDELLDADDAARAVRLAHIRATDSAVADD
                     IEALLAQQASVETAQFLEGSALALPAEATLAGKVVGSYTLERVIGQGGMGSVWLAHRS
                     DGRFEGRAAIKFLNLALLGHGGAERFRREGSVLARLAHPNVARLIDAGIHAGGQPYLI
                     LEYVDGEPIDRWCAARTLDIGARLRLFLDVLAAVAHAHSNLVLHRDLKPSNILVTASG
                     QVKLLDFGIAKLLEGDAQDATALTRHAGHAFTPEYAAPEHIAGTELTTAADVYSLGVV
                     LYELLTGKRPYQLKRASAAALVEAITREEPPLASSVASNEADRRRLRGDLDAILNFAL
                     KKRPEERYQTVAELAADIQRHLNGVPVRAQPDRLAYRVAKFVRRHRIGVSAAGAVVLA
                     VLAGGGAALWQAHEATRQRDRALSLSARNEAVVNFVNEMLTEVAPTDRPIMVNELLER
                     SLSMLTAAGGNVEHQAAVLGMLANYFLSTGNPAKAAPLLERALELTRATAVTALRARL
                     LCENADAAQLLGHPDAAARAIEEGMKLARGDPLAAAWCLQKRASIAQQSNDAKSALEF
                     ALQARMQLRESPIANPELDATLLADIAAAHYLTGQAAEADRY"
     gene            complement(1878..2450)
                     /locus_tag="AC16HB_02350"
     CDS             complement(1878..2450)
                     /locus_tag="AC16HB_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR5605660.1"
                     /translation="MDEPSVDVQTLVTALAAAERGDAAATADLFALLYGELRRLARRQ
                     LHANASGLTLGATTLLHEAYLDISGRGAAFPDRARFFAYAARAMRGLIIDYVRERRAL
                     KRGGEFHLTALDTEAAESIPEPTELTHLDEALAELAQAEPELAELVDLKYFCGFSFAE
                     IASMRNVSERTVQRDWAKARLYLHDALRAD"
     gene            2757..3590
                     /locus_tag="AC16HB_02355"
     CDS             2757..3590
                     /locus_tag="AC16HB_02355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5605661.1"
                     /translation="MNAVIDKPVATPDLATIKQRQQATWASGDFAVIGTTLQIVGESL
                     AEAADIRAGEHVLDVAAGNGNATLAAARRFAHVTSTDYVPALLDKGRARAQAEGLAVQ
                     FKVADVEALPFDDGCFDAVLSTFGAMFAPDQARTAREMLRVVRPGGRIGLANWTPEGF
                     IGRLFKLIGGYLPPPAGLASPALWGTEQHLFGLFGPQAAQICCARRTFNFRYRSAAHW
                     VQVFRDYYGPTHKAFAALDASRQTELERAITALLERMNTAGSDSLVVPAEYLEIVITK
                     C"
BASE COUNT          547 a         1322 c         1261 g          586 t
ORIGIN      
        1 gtagcgatcc gcctcggcag cctgtccggt caggtaatgc gcggccgcga tgtcggccag
       61 cagcgtggcg tccagttccg gattcgcgat gggcgattcg cgcagttgca tccgagcctg
      121 cagcgcgaac tcgagcgcag acttcgcgtc gttcgactgc tgggcgatgc tggcgcgctt
      181 ctgcaggcac caggcggccg cgagcggatc gccgcgggcg agcttcatgc cctcttcgat
      241 cgcgcgcgcg gcagcgtcgg ggtgcccgag cagctgtgcg gcgtcggcgt tctcacacag
      301 cagtcgcgcg cgcagcgccg tgacggcggt cgcgcgcgtc agttcgagtg cgcgttccag
      361 cagcggcgca gccttggccg ggttgccggt cgacaggaaa tagttcgcga gcatgccgag
      421 aaccgccgcc tggtgctcca cgttgccgcc ggccgccgtc agcatcgaca ggctgcgctc
      481 gagcagttcg ttgaccatga tcggccggtc ggtcggcgcg acctcggtca gcatctcgtt
      541 cacgaaattg accaccgcct cgttgcgcgc cgacagcgac agcgcgcggt cgcgctggcg
      601 cgtggcctcg tgcgcctgcc acagcgcggc gccgccgccc gcgaggaccg ccagcacgac
      661 ggcccccgca gcgctcacgc cgatgcgatg ccgccggacg aacttcgcga cgcgatacgc
      721 gagccgatcc ggctgcgccc gtaccggcac gccgttcagg tggcgctgga tgtccgccgc
      781 cagttcggca accgtctggt aacgctcttc ggggcgcttc ttcagcgcga aattcaggat
      841 tgcgtccagg tcgccgcgca gccggcggcg gtcggcttcg ttcgacgcga cgctgctcgc
      901 gagcggcggc tcctcccgcg tgattgcttc gaccagcgcg gcagcgctcg cgcgcttgag
      961 ctggtacggc cgcttgccgg tcagcagttc gtacagcacc acgccgagcg aatagacgtc
     1021 ggccgcggtc gtcagctcgg ttccggcaat gtgctccggc gccgcgtact ccggcgtgaa
     1081 ggcgtgaccg gcatggcgcg tcagcgcggt ggcgtcctgc gcgtcgcctt cgagcagctt
     1141 ggcgatgccg aagtcgagca gcttcacctg cccgctcgcg gtgacgagaa tgttcgaggg
     1201 cttcaggtcc cggtgcagca ccagattgct gtgggcgtgc gcgaccgcgg ccagcacgtc
     1261 gagaaacagg cgcagccgcg cgccgatgtc gagtgtgcgc gccgcgcacc agcggtcgat
     1321 cggctcgccg tcgacgtact cgaggatcag gtagggctga ccgcccgcat gaataccggc
     1381 gtcgatcagg cgcgccacgt tcggatgcgc cagccgcgcc agcacgctgc cttcgcgccg
     1441 gaagcgttcc gcgccaccgt ggcccagcag cgcaaggttc aggaacttga tcgcggcgcg
     1501 gccttcgaag cgcccatcgc tgcgatgagc cagccatacg ctgcccatgc cgccctgccc
     1561 gatcacgcgc tcgagcgtat agctgccgac gaccttgccc gccagcgttg cctcggccgg
     1621 cagcgccagc gcggagccct ctaggaactg cgcggtttcg accgacgcct gctgcgcgag
     1681 cagcgcttcg atgtcgtcgg ccaccgcgct gtccgtggca cggatatggg cgagccgcac
     1741 cgcacgcgcg gcgtcgtccg cgtccagcag ttcgtcgagc agcggactga tgcgtgccca
     1801 gcgttgtcgg tcgatgcccg ccatgattgc gccctttacc gactcgatcg gattgcgccg
     1861 gcgaaacccg acagcggtca gtcagcgcgc agcgcgtcgt gcaggtacag gcgcgccttg
     1921 gcccagtcgc gctgcacggt ccgttcggac acattgcgca tcgacgcgat ctctgcgaac
     1981 gagaatccgc agaagtactt caggtcgacc agctcggcca gttcgggctc ggcctgcgcc
     2041 agctcggcca gcgcctcgtc gaggtgcgtc agttcggttg gctcgggtat cgactccgcc
     2101 gcttcggtat cgagcgcggt cagatgaaat tcgccacccc gcttcagtgc ccggcgttcg
     2161 cgcacgtaat cgatgatcag cccgcgcatg gcgcgcgccg catatgcgaa gaagcgtgcg
     2221 cgatccggaa acgcagcgcc gcgcccggag atatcgaggt acgcctcatg cagcagtgtg
     2281 gtggcgccga gcgtcagtcc ggacgcgttg gcatgcagct ggcgccgtgc caggcgccgc
     2341 agctcgccgt acagcaacgc gaacaggtcc gccgtggcgg ccgcgtcgcc gcgctcggcg
     2401 gccgccagcg cagtgacgag cgtctgcaca tcgactgacg gttcgtccat cgcctggtgt
     2461 cctgctgacg tgggctgccg ggaaagccgc gcgcgcacgt ggcgcgggcg gcgggacgcg
     2521 aatgataaat cgcggctccc gcggtcgggt cggctttctc cccgcctatc caatccggga
     2581 ctaacggatc gaagtcactt cgacggcggc tcgagccgca acgcggcttc gccggctgtc
     2641 gccttttgac gccgaagtcc gattgactcg gcagtggcga tcggcgcgga cgtggaacgc
     2701 cgctgcaacg ccgagcgccc gaccatccgg cttcacttac ctcgaggagt tcacgcatga
     2761 atgcagtcat cgacaagccc gtggcgacac cggatctggc cacgatcaag cagcgtcagc
     2821 aggctacctg ggccagcggc gacttcgccg tcatcggcac gacgctgcag atcgtcggcg
     2881 agtcgctcgc cgaggccgcc gacatccgcg ccggcgagca cgtgctggac gtggccgccg
     2941 gcaacggcaa cgccacgctg gccgccgcgc gccgcttcgc ccacgtcacc tccaccgact
     3001 acgtgcccgc gctgctggac aagggccgtg cacgcgccca ggccgagggt ctggcagtgc
     3061 aattcaaggt ggccgacgtg gaggcgctgc cgttcgacga cggatgcttc gatgccgtgc
     3121 tgtcgacctt cggcgcgatg tttgcgcccg accaggcgcg cacggcccgc gagatgctgc
     3181 gcgtcgtgcg accgggcggc cgcatcggtc tggcgaactg gacgccggaa gggttcatcg
     3241 gaaggctgtt caagctgatc ggcggctacc tgccgccgcc cgcgggcctg gcctcgcccg
     3301 cactgtgggg caccgagcag cacctgttcg gcctgttcgg accgcaagcc gcgcagatct
     3361 gctgcgcacg gcgcaccttc aacttccgtt accgctcggc ggcacattgg gtgcaggtgt
     3421 tccgtgacta ctacgggccg acccacaagg cgttcgcggc gctggatgca tcccgccaga
     3481 ccgaactgga gcgcgccatc acggcgctgc tggaacgcat gaacaccgcc ggctccgact
     3541 ccctcgtcgt cccggccgag tacctcgaga tcgtgatcac caaatgctga ggaccgccat
     3601 gaaccattcc agctacgccg tgctttcgat cgcgctggcg ctcgcgggtt gcgcgagtgc
     3661 gctgcccgcc aacgtcccgc agcaactgca gcccgaggcc gccgaccctc acccgc
//
LOCUS       DBNHBY010000098        14226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_614329, whole genome shotgun sequence.
ACCESSION   DBNHBY010000098 DBNHBY010000000
VERSION     DBNHBY010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_614329"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC16HB_02360"
     CDS             complement(<1..662)
                     /locus_tag="AC16HB_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR5605662.1"
                     /translation="MISTPHPLDRDIIVNTLGHARTPLTPDDLAQRLKVSEAQRAQFE
                     SDLVELERAGQIVRNRAGLLLLAARAHLLSGQVQGHRDGFGFVIRDDGGPDLVLPEHE
                     MSKVLHGDRVLVRVSGQDRRGRTEAEIVEVIEHRTNRLVGRLLNERGVTIVVPEDQRI
                     QHDILVPPGGALSGQHGQVVVVQIVQQPQRHVQPVGRVVEVLGAIDDPGMEIEIAVRK
                     FAV"
     gene            748..832
                     /locus_tag="AC16HB_02365"
     tRNA            748..832
                     /locus_tag="AC16HB_02365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:782..784,aa:Leu,seq:cag)
     gene            complement(881..1393)
                     /locus_tag="AC16HB_02370"
     CDS             complement(881..1393)
                     /locus_tag="AC16HB_02370"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5605663.1"
                     /translation="MEHLYVEWDEYHRLIERLALRVYESGFKFDAVLCLARGGLRIGD
                     VFSRIYDRPLAILATSSYRDAAGTQQGQLDIAEFITMTGGQLRGKVLLVDDLVDSGVT
                     LQRVTAHVRKRFPEIAEVRTAVLWHKAVSMFKPDYSVDYLPTSPWIHQPFEEYDAMRP
                     NQLAARLRQP"
     gene            complement(1402..2697)
                     /locus_tag="AC16HB_02375"
     CDS             complement(1402..2697)
                     /locus_tag="AC16HB_02375"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR5605664.1"
                     /translation="MAKNVVIVGTQWGDEGKGKIVDWLTEHSQGVVRFQGGHNAGHTL
                     VIQGRKTILRLIPSGILRPDVACYIGNGVVLSPQALLAEIDELTAAGVACTQRLAISG
                     NCPLILEYHVALDQAREERKGAGKIGTTGRGIGPAYEDKVGRRAVRAQDLFDAGRFAE
                     RVREALDLHNFVLTRYLGAAAVDAEAVIDANLKLGERIKPMLTDVSYRLNQVMADGHR
                     LLFEGAQGTLLDVDHGTYPYVTSSNTVAGAASAGAGVAPQTLQYVLGITKAYATRVGS
                     GPFPTELTDATGEHLRAKGKEFGSVTGRPRRCGWFDAAALKRAVQINGLRGLCITKLD
                     VLDGLPTVRLCTGYGYEGKVADILPYGADQVARCEPVYEEFPGWSESTFGVRDWNGLP
                     ANAQRYLERLAEVTGVPIDLVSTGPDREQTIVLRQPFND"
     gene            complement(2717..3856)
                     /locus_tag="AC16HB_02380"
     CDS             complement(2717..3856)
                     /locus_tag="AC16HB_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR5605665.1"
                     /translation="MARWLLPEGISDVLPQEARRLEQMRRALLDLFARWGYELVIPPL
                     IEYVDSLLTGSGADLDLRTFKLIDQMSGRMLGLRADTTPQVARIDAHILNRSGVVRLA
                     YASQVLHARPLHPLASREPFQVGAELYGHAGLAADAEVLQLAMLALATLGLAGARFDL
                     GHTGVLRAVLASDADAQRRTDDIVAALSSKDGAALAVAIDGLSSAAQQGLRTLLKLHG
                     GLDILAHARRELPSRPDITAALDELEALARQAGAAELSLDLADLHGYRYHTGITFAAY
                     LPGPPGAVLRGGRYDDIGRAFGRARPATGFSIDLRDLTRVDTNGDNRAILAPPGADAA
                     LITRLRDAGETVVQQLAHDEVPAGHCFDRELRSVAGAWQVVPRGG"
     gene            complement(3860..4045)
                     /locus_tag="AC16HB_02385"
     CDS             complement(3860..4045)
                     /locus_tag="AC16HB_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR5605666.1"
                     /translation="MLSTFVLALALMLVIEGALPLLAPAVWRDMFRRMMELKDGQIRF
                     FGLASIAIGLALYWLAS"
     gene            complement(4045..4929)
                     /gene="hflC"
                     /locus_tag="AC16HB_02390"
     CDS             complement(4045..4929)
                     /gene="hflC"
                     /locus_tag="AC16HB_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR5605667.1"
                     /translation="MNRLVGTLVGIVVAAVLFRSMAFIVDQRQYAVLFAFGQIKEVIS
                     EPGLHFKLPPPFQNVVYLDKRILTIDTPQSDRVQTSEKKNLLVDSFVKWRISDARRYW
                     VSFGGSERAAQERVSTLVRDAMNQAINKRTVNDVTSRERDRAMEEIRVAVQERVKDVG
                     VEIVDVRLKRVDFVPEISESVFRRMEAERKRVANEQRSIGAAESEKVRADADRQREVL
                     LAEAYRDAQKTKGDGDAKASAIYAQAFGQNAEFFAFYRSLEAYRSAFRNRSDVLVVDP
                     SSDFFKYMKNPGTGRPGR"
     gene            complement(4939..6210)
                     /gene="hflK"
                     /locus_tag="AC16HB_02395"
     CDS             complement(4939..6210)
                     /gene="hflK"
                     /locus_tag="AC16HB_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR5605668.1"
                     /translation="MFHVSGIAMSLNDPQWGRGGGTDNNSSSEEQRRTGKPGGQDGPP
                     DLDELLRDFNRRLNTLFGRRGGNGEGGGRGGGFRPDARGAGIGVLAILAAVFVIWMGS
                     GFYIVQEGQAGVVTTFGRYSESTTAGFRWRAPWPIQAHEIVDVLKLQTVEIGARGRAD
                     RLKEALMLTDDENIVDIHFTVQYRIKEGPNGARDFLFNSRNPTEAVTQTAESAMREVV
                     GRKMMDSVLYESRQEIADQVRGRMQEMLDRYGTGILISAVAIQNAQPPEQVQAAFDDA
                     VKASQDRERQINEGQAYANDVVPKARGLAARLTQEAEGYRTRVTETAQGDASRFRQVL
                     VEYNKAPAVTRERLYLETMQQVFSNTTKVLVDSKSGNQLLYLPFDRLMQQAIAESGGA
                     RVAAPATPEPAPPAAEGRSRDNQRSRERETR"
     gene            complement(6251..7378)
                     /gene="hflX"
                     /locus_tag="AC16HB_02400"
     CDS             complement(6251..7378)
                     /gene="hflX"
                     /locus_tag="AC16HB_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR5605669.1"
                     /translation="MLVSVTLSRRPSGVHAARDEVEELRQLATSAGFEIRGEAAVRRE
                     RPDPQHYVGSGKVEQLRVEALARDAGVVIFDVALTPAQQRNLERALGAWVLDRTALIL
                     EIFGQRAKSREGKLQVELAQLEHLSTRLVRGWTHLERQRGGLGKTGGPGEKQIELDRR
                     MIASRVRLLRERLKILHRQRRTQRRARARRDVYSVSLVGYTNAGKSTLFNALARASSY
                     AADQLFATLDTTSRRCFVADGVEVVLSDTVGFIRGLPHQLVEAFKSTLEESVEADLLL
                     HVVDAAAPARDMQMAEVQRVLEEIGADQVPQLIVYNKIDALERAPAVERDPYGRMLAV
                     SVSAMTGAGLDLLRAAIAECVRTSRAEAPIPDFESAAASSA"
     gene            complement(7426..7659)
                     /gene="hfq"
                     /locus_tag="AC16HB_02405"
     CDS             complement(7426..7659)
                     /gene="hfq"
                     /locus_tag="AC16HB_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR5605670.1"
                     /translation="MSNKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPARPVNLSAETAS"
     gene            complement(7821..9140)
                     /gene="der"
                     /locus_tag="AC16HB_02410"
     CDS             complement(7821..9140)
                     /gene="der"
                     /locus_tag="AC16HB_02410"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5605671.1"
                     /translation="MPVVAIVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRHYGDG
                     RVGPVPYLVIDTGGFEPQGAAGIAREMAQQAELAIAESDVVIFVVDAREGLTAQDRRI
                     AQRLRETGRRLVIAVNKAEGLPPEASAEFHELGLGEPRPISAAHGDGVAGMMDELLAG
                     FEAPQQEAEPAVTQPIRVAIVGRPNVGKSTLANALLGENRLIAFDEPGTTRDAIEVDF
                     EFGGQQFRLIDTAGLRRKGKVFETIEKFSVVKTLQSIERCHVALLLLDGAAEIADQDA
                     HIAGYILEKGRALVIAINKWDGLDEYQRGLVKRELDRKLHFLRWARVHYISALQGTGL
                     AAVMRSVAGAHRAAFTKLPTPKLTRALHAAVERQMPPRAGASRPKMRYAHQGGQNPPT
                     IIIHGNRLNHVADSYRRYLEGWFRDVFDLHGTPLRIEFRTSANPYAS"
     gene            complement(9157..10284)
                     /gene="bamB"
                     /locus_tag="AC16HB_02415"
                     /gene_synonym="yfgL"
     CDS             complement(9157..10284)
                     /gene="bamB"
                     /locus_tag="AC16HB_02415"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR5605672.1"
                     /translation="MTQRWSPWQQCAAVLVLVGCASSTSTNKPAELAEFKSSLDVRVT
                     WRASIGSGRGTFLQPAVLENAIYAASADGALQRIDPQTGAVVWRVSVEAPLSAGVGSD
                     GFAIAVATPRGEVIAVGADGKPLWRAQASSDVVAPPLVGRGLVVVRSTDHRVTAYEAD
                     SGKRRWTYQRQSPPLTLRTSTEMAFAGDNVLVGLPAGRLIALALTNGAARWEAIVSEP
                     KGTTEVERLNDVVGPIAVDARDACAASFQGRVMCADPANGNLRWARELSARGGVSMNE
                     RAVYAVDNGSNVQAFARDGASLWRNNKLAWRAVTTPLALPGGAAVGDRSGYVHFLGSD
                     GEFIARVQIDSSAIVARPLRWADGVIVLTQDGTLALLTPQR"
     gene            complement(10281..10916)
                     /locus_tag="AC16HB_02420"
     CDS             complement(10281..10916)
                     /locus_tag="AC16HB_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR5605673.1"
                     /translation="MAYDLQEQEQLASMKAWWDKYGNFILTTATIVLLAIAAFNGWRW
                     YERREAQQAAVVYDELLRTLDGTDANRKKELGGTLIERYGRTVYAPMAALQMARFYHD
                     AGDNATARAQLQWVIDKSGHNEYVLVARVRLAGVLLDDKAYDEALKVLAVDVPRSHAT
                     AFADRRGDVLVAQNKLDEARASYREALDKADAQNPLRNLIQLKLDALPAAS"
     gene            complement(10926..12140)
                     /gene="hisS"
                     /locus_tag="AC16HB_02425"
     CDS             complement(10926..12140)
                     /gene="hisS"
                     /locus_tag="AC16HB_02425"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5605674.1"
                     /translation="MADWLRSYGYQQIRTPIVEYTELFVKGIGEVTDIVEKEMYSFED
                     KLNGEHLTLRPEFTAGVIRAAVEHNLTYDAPRRVWSMGPVFRHERPQRGRYRQFHQVN
                     VEALGFAGPDIDAELIVMLARLWKILGIERIRLELNCLGQADERARHRADLIVYLSRY
                     AAQLDDDARRRLHTNPLRILDTKNPALAEIVAGAPHITEYLGDASRAHFDGLRAQLAS
                     AGIEFTVNPRMVRGLDYYNLTVFEWITDALGAQGTLCGGGRYDPLIERMGGRPTPGIG
                     FGLGVERVIELLREAGRVQAAPACDVYVLHQGSATLVAAMHAAELLRDAGLDVILHAG
                     EGGLKSQMRKADASGAEFALIVGEAEAAQQMAAVKALRARGADAPFATQVQVPLAQVG
                     DALVTAMERLSE"
     gene            complement(12228..13508)
                     /gene="ispG"
                     /locus_tag="AC16HB_02430"
                     /gene_synonym="gcpE"
     CDS             complement(12228..13508)
                     /gene="ispG"
                     /locus_tag="AC16HB_02430"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR5605675.1"
                     /translation="MNQPTEAWNCLSLRAAPTPRRATRQVQVRWGARVVRIGGDAPVM
                     VQSMTNTDTADAIATAIQVKELFLAGSEVVRITVNSAEASAEVPAIRAQLDQMGVDVP
                     LVGDFHYNGHKLLAEFPACAQALSAYRINPGNVGRGAKREDNFARMIEVACRHEKPIR
                     IGVNWGSLDQELLARMMDENAARAEPWDAGSVLREALVRSAIENAQRAEELGLGRDMI
                     VLSAKVSGVQDLVAVYRELSRRCDYPLHLGLTEAGMGSKGIVASTAALAVLLQEGIGD
                     TIRVSLTPAPGEPRTREVEVAQQILQAMGLRAFTPQVIACPGCGRTTSTFFQELAQRI
                     QDYLRAQMPGWRARYPGVESMRVAVMGCVVNGPGESKHANIGISLPGSGEAPVAPVFV
                     DGVRTVTLKGEAIAEEFQAIVADYVRRRYARAGV"
     gene            complement(13518..>14226)
                     /locus_tag="AC16HB_02435"
     CDS             complement(13518..>14226)
                     /locus_tag="AC16HB_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5605676.1"
                     /translation="ARLRISAKQVSALEREDLAALPGTAFVRGIVRNYAKEVRIDPAP
                     LLASLNRMLEPASAETPVRATLLRTAERERGSRTLVIAGAIAALIVFAVIGWIANTRS
                     RQAAAPAVADTATRATAPSTTPSATTTGDTAGPAPTEITAPSLPASQPAAAPSEPGAL
                     RFGFRDIAWVEVTQQDGAVLLAQNNPAGSEQIVRGKPPYQIVIGNASSVTLEYEGKPI
                     DLKPVTGVANVARLMVK"
BASE COUNT         2231 a         5008 c         4584 g         2403 t
ORIGIN      
        1 accgcgaatt tgcgcaccgc gatctcgatc tccatgccgg gatcgtcgat cgcaccgagc
       61 acctcgacca ctcggcccac gggctgcacg tgccgctgcg gctgctgcac gatctgcacc
      121 accacgacct ggccatgctg ccccgacagc gcaccgccgg gcggaaccag gatgtcgtgc
      181 tgaatgcgtt gatcctcggg cacgacgatc gtcacgccgc gctcgttcag cagccgtccg
      241 acgaggcgat tggtccggtg ctcgatgacc tcgacgatct cggcttcggt ccgtccacgc
      301 cggtcctgac cgctgacgcg cacgagcacg cggtcgccgt gcagcacctt gctcatctca
      361 tgctcgggca gcaccagatc cggtccgccg tcgtcgcgga tcacgaaccc gaagccgtcg
      421 cgatgcccct gtacctggcc cgacagcaag tgcgcgcgcg ccgccagcag cagcagcccg
      481 gcgcgattgc ggacgatctg accggcgcgc tcgagttcca cgagatccga ctcgaactgc
      541 gcgcgctgcg cttcgctgac tttcagtcgc tgggcaagat cgtccggcgt cagcggcgtg
      601 cgtgcatgcc ccagcgtgtt gacgatgatg tcgcgatcga gcgggtgtgg cgtggaaatc
      661 aagcgaggct ttcgttcaga atgactgcgc cggccgcgtc aatttgacat gcaaaggccg
      721 gaccttacaa ttcgcgcctc gcgccgtgcc caggtggcgg aattggtaga cgcactagtt
      781 tcaggtacta gcgccgcgag gtgtggaggt tcgagtcctc tcctgggcac caaattagaa
      841 gccagcgcca gctggctttt tgttttgatg cgcccgcaag tcacggctgg cgcagccgtg
      901 ccgcgagctg attcggccgc atcgcgtcgt attcctcgaa cggctggtgg atccacgggc
      961 tcgtcggcag atagtcgacg gagtaatcgg gcttgaacat cgacaccgcc ttgtgccaga
     1021 gcacggccgt gcgcacctcc gcgatctccg gaaagcgctt gcgcacatgc gcggtcaccc
     1081 gctgcagcgt cacgccggaa tcgacgaggt cgtcgaccag cagtaccttg ccccgcaact
     1141 ggccgccggt catcgtgatg aactccgcga tgtcgagctg cccttgctgg gtgccggcgg
     1201 cgtcccggta cgaactcgtc gccaggatcg caagcggccg atcatagatg cgcgaaaaca
     1261 cgtcgccgat gcgcaatccg ccgcgcgcca ggcacagcac cgcgtcgaac ttgaagccgg
     1321 actcgtaaac tcgcaacgcg agcctttcga tcaggcggtg atactcgtcc cactcgacgt
     1381 acaggtgctc cattgcggca ttcagtcgtt gaatggctgc cgcagaacga tcgtctgctc
     1441 gcgatcggga ccggtggaaa ccaggtcgat cggcacaccg gtgacttcgg cgaggcgctc
     1501 gagatagcgc tgcgcattgg cgggcaggcc attccagtcg cgaacgccga acgtcgattc
     1561 gctccagccc ggaaattcct cgtacacggg ctcacagcgc gcgacctggt cggcgccgta
     1621 gggcaggatg tcggcgacct tgccttcgta accgtagccg gtgcacagcc gcacggtcgg
     1681 caagccgtcg agcacgtcga gtttggtgat gcacaagcct cgcaggccgt tgatctgcac
     1741 tgcgcgcttc agcgccgcag cgtcgaacca gccgcagcgg cgcggtctgc cggtaaccga
     1801 gccgaattcc ttgcccttcg cgcgcagatg ctcgccggtc gcgtcggtga gttcggtagg
     1861 gaacgggccc gagccgaccc gcgtcgcgta ggccttggtg atgccgagca cgtattgcag
     1921 ggtctgcggc gccacaccag ccccggccga ggccgcgccg gcgaccgtgt tgctggaggt
     1981 cacgtacggg taggtgccgt gatcgacgtc gagcagcgtg ccctgcgcgc cttcgaaaag
     2041 caggcggtgc ccgtcggcca tcacctggtt cagccgatac gacacgtcgg tcagcatcgg
     2101 cttgatccgc tcgccgagct tgagattcgc atcgatcacc gcctcggcat cgaccgccgc
     2161 tgcgccaagg tagcgggtca gcacgaaatt gtgcaggtcg agcgcctcgc gcacgcgctc
     2221 ggcgaagcgg ccggcgtcga acaggtcctg tgcgcgcacc gcgcggcggc cgaccttgtc
     2281 ctcgtatgcc gggccgatcc cgcggcccgt ggtgccgatc ttgcccgcgc ccttgcgctc
     2341 ctcgcgcgcc tggtcgagcg cgacgtggta ctcgaggatc agcgggcagt tgccgctgat
     2401 cgccagccgc tgcgtacacg caacgccggc cgcggtcagt tcgtcgatct ccgccagcag
     2461 cgcctgcggg gacagcacca cgccgttgcc gatgtagcac gcgacgtcgg gccgcagaat
     2521 gcccgacggg atcaaccgca ggatcgtctt gcgaccctgg atcaccagcg tatggcccgc
     2581 gttatggccg ccctggaagc gcaccacccc ttgcgaatgc tcggtcagcc aatcgacgat
     2641 cttgcccttg ccttcgtcgc cccactgggt gccgacgatg accacgttct ttgccatcgg
     2701 tgttccgttc tctcgctcag ccgccgcgcg gcacgacctg ccatgccccg gccacgcttc
     2761 gaagctcgcg atcgaagcag tgacccgccg gcacctcgtc gtgcgccagt tgctgcacga
     2821 ccgtttcgcc ggcgtcgcgc agccgcgtga tcagcgcggc gtccgccccc ggcggcgcaa
     2881 gtatcgcccg gttgtccccg ttcgtatcga cgcgcgtcaa atcacgcaga tcgatcgaga
     2941 agcccgtcgc cgggcgcgcc ctgccgaatg cccgcccgat atcgtcgtaa cgcccgccgc
     3001 gcagcaccgc gccgggcggt cccggcaggt acgcagcaaa ggtgatgccg gtgtggtagc
     3061 ggtagccatg caggtcggcc agatcaaggg acagttcggc cgcaccggcc tgccgtgcca
     3121 gcgcctcgag ctcgtccagc gccgcggtga tgtccggtcg cgacggcagt tcgcggcgtg
     3181 cgtgcgccag gatgtcgagt ccgccgtgca acttcagcag cgtgcgcagg ccctgctgcg
     3241 cagcggacga caggccgtcg atcgctaccg ccagtgccgc gccatccttg ctgctcagcg
     3301 ccgcgacgat gtcgtcggtc cgccgctgcg cgtcggcgtc cgacgccagc accgcgcgca
     3361 gcacgccggt gtggcccagg tcgaagcgcg cgcccgcgag gccgagcgtg gccaacgcca
     3421 gcatggcgag ttgcagcact tcggcatccg ccgcgagacc tgcgtgaccg tacagttcgg
     3481 caccgacctg gaacggctca cgcgaggcca gcggatgcag cgggcgcgca tgcagcacct
     3541 gcgacgcgta ggccagccgc accacgccgg accggttgag gatgtgcgca tcgatgcgcg
     3601 ccacctgtgg cgtcgtatcg gcacgcagcc ccagcatgcg gccggacatc tggtcgatca
     3661 acttgaacgt gcgcaggtcg aggtcggcgc cggagcccgt cagcagcgaa tcgacgtact
     3721 cgatcagcgg cggaatcacc agctcgtaac cccagcgcgc gaacagatcg agcagtgcac
     3781 gccgcatctg ctcgagccgt cgcgcctcct gcggcagcac gtcggaaatg ccctcgggga
     3841 gcagccagcg tgccatcgct caactcgcga gccagtacag ggcgagcccg atcgcgatcg
     3901 acgccaggcc gaagaagcgg atctgcccgt ccttcagttc catcatgcgg cgaaacatat
     3961 cgcgccagac ggcgggcgcg agcagcggaa gtgctccctc gatcaccagc atcagcgcca
     4021 gcgccaggac gaacgtcgac agcatcagcg tccgggacgg cccgtgcccg gattcttcat
     4081 gtacttgaag aagtcagacg acggatcgac gaccagcaca tcgctgcggt tgcggaacgc
     4141 cgatcgatac gcctcgaggc tgcggtagaa ggcgaagaac tcggcattct gtccgaacgc
     4201 ctgcgcgtag atcgcggacg ccttcgcgtc gccgtcgccc ttggtcttct gcgcgtcgcg
     4261 atacgcctcg gccagcagca cctcgcgctg ccggtcggca tcggcgcgca ccttttcgct
     4321 ctcggccgcg ccaatcgagc gctgctcatt ggccacgcgc ttgcgctccg cctccatgcg
     4381 acggaacacg gactcgctga tctccggcac gaaatcgacc cgcttgagcc gcacgtcgac
     4441 gatctccaca ccgacatcct tcacgcgctc ctggacggcg acgcggatct cctccatcgc
     4501 ccggtcgcgc tcgcgcgacg tcacgtcgtt gacggtgcgc ttgttgatcg cctggttcat
     4561 cgcgtcgcgc accagggtcg atacgcgctc ctgggcagca cgctcgctgc cgccaaagct
     4621 gacccaatag cggcgcgcgt cgctgatgcg ccacttgacg aacgaatcga ccagcaggtt
     4681 cttcttctcc gacgtctgca cgcggtccga ctgcggcgtg tcgatggtca ggatgcgctt
     4741 gtcgaggtag acgacgttct ggaacggcgg cggaagcttg aagtgcaggc cgggctcgct
     4801 gatcacttcc ttgatctgcc cgaacgcgaa caagaccgcg tactgacgct gatcgacgat
     4861 gaaggccatc gagcggaaca gcaccgctgc cacgacgatg ccgaccaggg tgccgacgag
     4921 tcgattcatc gcggtctcct agcgcgtctc gcgttcgcgg ctgcgctggt tgtcgcggct
     4981 gcgaccttcg gccgctggcg gcgccggctc gggcgtggcc ggtgcggcca cacgcgcccc
     5041 ccccgactcg gcgatcgcct gctgcatcag ccggtcgaac ggcaggtaca gcagctggtt
     5101 gccgctcttc gaatcgacca ggaccttggt cgtgttgctg aatacctgct gcatcgtttc
     5161 caggtacagg cgctcgcgcg tcacggccgg cgccttgttg tactcgacca gcacctggcg
     5221 aaagcgcgat gcatcgccct gcgctgtttc cgtcacgcgg gtccgatagc cttcggcctc
     5281 ctgcgtcagc cgcgcggcga ggccccgcgc cttgggtacg acgtcgttgg cgtaggcctg
     5341 gccctcgttg atctgccgct cgcggtcctg cgatgccttg accgcgtcgt cgaacgccgc
     5401 ctgcacctgc tcggggggct gtgcgttctg gatcgccacc gccgagatca ggatccccgt
     5461 gccataccga tcgagcatct cctgcatgcg tccgcggacc tggtcggcga tctcctgccg
     5521 cgactcgtac agaacgctgt ccatcatctt gcggccgacc acctcgcgca tcgcggactc
     5581 cgcggtctga gtcaccgcct cggtcgggtt gcggctgttg aacaggaaat cgcgtgcgcc
     5641 gttcggcccc tccttgatgc ggtactggac cgtgaagtgg atgtcgacga tgttctcgtc
     5701 gtcggtcagc atcagcgctt ccttcagccg gtcggcccgt ccgcgcgcgc cgatctcgac
     5761 cgtctgcagc ttcagcacgt cgacgatctc gtgcgcctgg atcggccacg gtgcgcgcca
     5821 gcggaagcct gccgtcgtgc tctccgagta gcggccaaac gttgtgacga cgccggcctg
     5881 cccttcctgc acgatgtaga agccgctgcc catccagatc acgaacacgg ccgcgagaat
     5941 ggcaagtacc ccgatgcccg cgccgcgtgc atcgggtcgg aagccgccgc cgcgcccgcc
     6001 tccttctcca ttgccgccgc gccggccgaa cagggtattc aggcggcgat tgaagtcgcg
     6061 caaaagttcg tcgaggtcgg gcgggccgtc ctgcccgccc ggcttgccgg tgcggcgctg
     6121 ctcctcgctg ctgctgttgt tgtcggtgcc gccgccgcgg ccccactgcg ggtcgttcag
     6181 cgacatcgca atgcccgata cgtggaacat gaaagtattg ttttgccgcg ttcgcctgcg
     6241 cgcacgccga tcaggccgaa gacgccgccg cagattcaaa atcagggatt ggcgcctccg
     6301 cccgcgacgt ccgaacgcac tcggcaatgg ccgcgcgcaa gagatcgaga cccgcgcccg
     6361 tcatcgcact gacagacacc gcaagcatcc taccataggg gtcacgttcg accgccggcg
     6421 cgcgctccag tgcatcgatc ttgttgtaga cgatcaactg cggcacctgg tcggccccga
     6481 tctcttccag cacgcgctgc acttcggcca tctgcatatc gcgcgctggc gccgcggcat
     6541 ccaccacgtg gagcagcagg tcggcctcga ccgactcttc cagcgtggac ttgaacgcct
     6601 cgacgagttg atgaggcagc ccgcggatga aaccgaccgt gtcggacagc accacctcca
     6661 cgccatcggc gacgaagcaa cgtcgcgacg tggtgtcgag cgtggcgaag agctgatctg
     6721 ccgcgtagct cgatgcccgg gccagcgcgt tgaacagggt cgacttgccg gcgttcgtgt
     6781 agccgaccag cgacaccgag tacacatcgc ggcgcgcgcg agcgcgtcgc tgcgttcgcc
     6841 gctgccggtg caggatcttc agacgctcgc gcagcagacg cactcttgac gcgatcatgc
     6901 ggcggtccag ctcgatctgc ttctcgcctg gcccaccggt cttgcccaga ccgccgcgct
     6961 gccgctcgag gtgggtccag ccacgcacaa ggcgtgtcga caggtgctcg agctgggcca
     7021 gctcgacctg cagcttgcct tcgcggctct tggcgcgctg gccgaagatc tccaggatca
     7081 gcgccgtccg gtcgagcacc caggcaccca gcgcgcgctc gagattgcgt tgttgcgccg
     7141 gcgtcagcgc gacgtcgaag atcacgacgc ccgcgtcgcg tgccagcgct tcgacacgca
     7201 attgctcgac cttgcccgag ccgacgtagt gctggggatc agggcgctca cgccgcactg
     7261 ccgcctcgcc ccggatttca aacccggccg acgtagccag ctggcgcaac tcctccacct
     7321 catcgcgtgc ggcgtgcaca cccgacggtc ggcggctcaa agtgacactg acgagcaggg
     7381 cgcggctgtt gcggtcggcc tggcccgcct cgatctgaaa gacggtcagg acgccgtctc
     7441 cgcgctgagg ttgaccggcc gcgccggcac cacggtcgag atggcgtgct tgtagaccat
     7501 ctgcgtcacc gtattacgca gcaggacaac gtactgatcg aacgattcga tctgcccttg
     7561 cagcttgatg ccgttgacca ggtagatgga caccggcaca tgctccttgc gcagagcgtt
     7621 caggaacggg tcttgtagta gttggccctt gttgctcatc tgcactccga tcgtttcttt
     7681 tcattttggc gccacccaaa agccgggcgc cactcgcatg tacgcgttag atggagcccg
     7741 cgcgcaaaaa ttcaatctat cggaaaaggc cgtatgccgc taccgcgtgg cggcgcgagc
     7801 ccgatcgtcg gatcaatgac ctagctggcg taagggttcg cactggtgcg aaattcgatc
     7861 cgcaacggcg ttccgtgcag gtcgaagacg tcgcggaacc agccctccag atagcgtcgg
     7921 tacgaatcgg ccacatgatt caagcggttg ccgtgaatga tgatcgtcgg cgggttctgg
     7981 ccgccctgat gcgcgtagcg catcttcggg cgcgaggcgc ccgcgcgtgg cggcatctgg
     8041 cgctccaccg cggcgtgcag cgcgcgcgtg agcttgggcg tgggcagctt ggtgaatgcc
     8101 gcgcggtgcg cgccggcgac cgagcgcatc accgccgcaa ggcccgtacc ctgcagcgcc
     8161 gagatgtagt ggacgcgcgc ccagcgcaga aagtgcagct tgcggtcgag ttcgcgcttg
     8221 acgaggccgc gctggtactc gtccaggccg tcccatttgt tgatcgcgat caccagcgcc
     8281 cgccctttct cgagaatgta gccggcgatg tgcgcgtcct ggtcggcgat ctcggccgcg
     8341 ccatcgagca gcagcagtgc cacgtggcac cgctcgatcg actgcagtgt cttgacgacc
     8401 gagaacttct cgatggtctc gaacaccttg cccttgcggc gcaggccggc ggtgtcgatc
     8461 aggcggaact gctgcccgcc gaattcgaaa tcgacctcga tcgcgtcgcg cgtcgtgcca
     8521 ggctcgtcga acgcgatcag ccggttctct ccgagcagcg cgttggccag ggtcgacttg
     8581 ccgacgttgg gccgcccgac gatcgccacg cgtatgggtt gcgtaacggc gggctcggcc
     8641 tcctgctgcg gcgcttcgaa gcccgccagc agttcgtcca tcatcccggc gacaccatcg
     8701 ccgtgcgcgg cggagatcgg ccgcggctcg cccagcccga gttcgtgaaa ctcggcactt
     8761 gcctccggag gcagaccctc ggccttgttg accgcgatca cgagccggcg acccgtctct
     8821 cgcagccgct gcgcgatgcg gcgatcctgt gcagtcagcc cttcgcgcgc atcgaccacg
     8881 aagatgacga cgtcgctttc ggcgatcgcg agttccgcct gctgcgccat ctcgcgcgcg
     8941 atgccggcgg caccctgcgg ctcgaagccg ccagtatcga tcacgagata aggcaccggc
     9001 ccgacgcggc cgtcgccgta gtgccggtcg cgcgtcaatc cgggaaagtc ggcgaccagt
     9061 gcgtcgcgtg tgcgcgtcag acgattgaac agcgtcgact tgccgacgtt cggccgcccg
     9121 acaattgcaa cgaccggcat ctttggctcg gcggattcag cgttgcggcg tgagcagcgc
     9181 cagcgtgccg tcctgagtca ggacgatgac gccatcggcc cagcgcagcg gacgggcgac
     9241 gattgcgctc gagtcgatct gcacgcgggc gatgaactcg ccgtcgctgc cgaggaaatg
     9301 gacatagccg gaccggtcgc cgacggccgc cccgccggga agcgccagcg gcgtggtgac
     9361 cgcgcgccag gccaacttgt tgttgcgcca cagactcgcg ccgtcacgcg cgaacgcctg
     9421 cacgttcgaa ccgttgtcga ccgcgtatac cgcgcgctcg ttcatcgaca caccgccacg
     9481 cgcggaaagc tcgcgcgccc agcgcagatt gccgttggca ggatcggcgc acatcacgcg
     9541 accctggaac gacgccgcgc acgcgtcgcg ggcgtcgacc gcgatcggtc cgacgacatc
     9601 gttcaggcgc tcgacctcgg tcgtgccctt cggctcggaa acgatcgcct cccagcgcgc
     9661 cgcgccgttg gtcaaggcca gcgcgatcag ccgcccggcc ggcagcccga ccaggacgtt
     9721 gtcgccggca aacgccatct cggtcgaggt acgcagcgtc agcggcggcg actggcgttg
     9781 ataggtccag cgccgcttgc cggaatcagc ctcataggcg gtgacgcgat ggtccgtcga
     9841 gcgcacgacc accaggccgc ggccgaccag cggaggggcg acaacgtcgc tcgacgcttg
     9901 cgcacgccac agcggcttgc cgtcggcacc gaccgcgatc acctcgccgc gcggcgttgc
     9961 gaccgcgatc gcgaagccgt cgctgcctac gcccgccgac aacggtgctt cgaccgacac
    10021 gcgccagacg accgcgccgg tctgcggatc gatgcgctgc agcgcgccgt cggcgctggc
    10081 ggcatagatg gcgttctcga gcacggccgg ctgcaggaac gtcccgcgcc cgctgccgat
    10141 cgacgcgcgc cacgttacgc gcacatccag gctgctcttg aactcagcga gttcggccgg
    10201 cttgttggtc gaagtgctcg acgcgcagcc gaccagaacg agcacggccg cgcactgctg
    10261 ccacggcgac cagcgctgtg tcatgacgcc gccggcagtg cgtcgagctt cagctggatg
    10321 aggttgcgca gcgggttctg cgcatccgcc ttgtcgagcg cctccctgta cgacgcgcgc
    10381 gcctcgtcca gtttgttctg cgccaccagc acgtcgcccc tgcggtcggc aaacgccgtc
    10441 gcatgcgatc ggggtacgtc gacggccagc accttcagtg cctcgtcata cgccttgtcg
    10501 tccagcagca cgccggccag gcgcacccgc gcgaccagca catattcgtt gtgaccggac
    10561 ttgtctatca cccactgcag ctgcgcgcgc gcggtcgcgt tgtcgccggc atcgtgatag
    10621 aagcgcgcca tctgcagtgc cgccatcggc gcgtaaacgg tacgaccgta acgctcgatc
    10681 agcgtcccgc ccagctcctt cttccggttc gcgtcggtgc cgtcgagcgt gcgcagcaat
    10741 tcgtcgtaga ccacggctgc ctgctgcgcc tcgcgccgtt cgtaccagcg ccagccgttg
    10801 aacgccgcga tcgccagcag cacgatggtc gcggtggtga ggatgaagtt gccgtactta
    10861 tcccaccagg ccttcatcga ggccaattgt tcctgttcct gtaagtcgta tgccatgtca
    10921 gggtctcact cagaaagcct ttccatcgct gtcaccagcg catcgccgac ctgtgccagc
    10981 ggcacctgca cctgggtcgc gaatggtgcg tccgcgccgc gcgcccgcaa cgccttgacg
    11041 gccgccatct gctgcgccgc ttccgcttcg ccgacgatca gggcgaattc ggcgccgctg
    11101 gcatccgcct tgcgcatctg cgatttcagc ccgccctcgc cggcgtgcag gatcacgtcg
    11161 agtccagcat cgcggagcag ctcggcagcg tgcatcgcgg cgaccagtgt ggcgctgccc
    11221 tgatggagca cgtagacgtc gcaggcgggt gcggcctgaa cccggcctgc ttcgcgcagc
    11281 aattcaatca cccgctccac tcccaagccg aagccgatgc ctggcgtggg ccgcccgccc
    11341 atgcgttcga tcagcgggtc gtagcggcca ccgccgcaca gcgtgccttg cgcgccaagc
    11401 gcgtcggtaa tccactcgaa caccgtgagg ttgtagtagt cgaggccgcg caccatgcgt
    11461 gggttgaccg tgaactcgat gccagccgac gccagctgcg cccgcagtcc gtcgaaatgc
    11521 gcgcgcgacg catctcccag gtattcggtg atgtgcggcg caccggcgac gatctccgcc
    11581 agtgccggat tcttggtgtc gaggatgcgc agcgggttcg tgtgcaaccg ccgccgggca
    11641 tcgtcgtcga gttgagctgc gtagcgcgac aggtacacga tcaggtcggc gcgatggcgt
    11701 gcgcgttcgt cggcctggcc gaggcagttc agctcgagtc gtatgcgctc gatgccgagg
    11761 atcttccaca gccgggccag catcacgatc aactcggcgt cgatgtcggg tccggcgaag
    11821 ccgagcgcct cgacgttgac ctgatggaac tgccggtagc ggccgcgctg cgggcgctcg
    11881 tgtcggaaca ccgggcccat cgaccacacg cggcgcggcg catcataggt caggttgtgc
    11941 tcgacggccg cgcgaatcac gccggcggtg aattccggcc gcagtgtcag gtgctcaccg
    12001 ttgagtttgt cctcgaagga gtacatctcc ttctcgacga tgtccgtgac ctcaccgatg
    12061 cccttcacga acagttcggt gtactcgacg atcggcgtcc ggatctgctg gtatccgtag
    12121 ctgcgcaacc agtcagccac gcgggcctag aaccactccc atagcggcgc gtccgcggga
    12181 agcatgtcgt tcatgccgcg cacgccggac agcttcttgc ccatcgctta caccccggcc
    12241 cgcgcgtacc tgcgccgcac gtaatcggcg acgatggcct ggaactcctc cgcgatcgct
    12301 tcacccttca gcgtaaccgt gcgtacgccg tcgacgaaca cgggcgcgac cggcgcctcg
    12361 cccgagccgg gcagtgagat cccgatgttc gcgtgcttgc tctcgcccgg gccattgacc
    12421 acgcatccca tcaccgcgac acgcatcgac tcgacgccgg gatagcgggc gcgccagccc
    12481 ggcatctgtg cgcgcaggta atcctggatc ctctgtgcga gttcctggaa gaacgtgctg
    12541 gtggtgcgac cgcagccggg gcaagcgatc acctgcggcg tgaacgcacg cagccccatc
    12601 gcctgcagga tctgctgcgc aacctcgact tcccgcgtgc gcggttcgcc gggcgccggc
    12661 gtgagcgaca cgcggatcgt gtcgccgatg ccctcctgca gcagcaccgc gagcgccgcg
    12721 gtcgacgcga cgatgccctt gctgcccatt cccgcctcgg tgaggccgag atgcagcggg
    12781 taatcgcagc ggcgcgacag ttcgcggtac acggcgacca ggtcctgcac gccgctgacc
    12841 ttggccgaca gcacgatcat gtcacgcccg aggccaagct cctccgcgcg ctgcgcattc
    12901 tcgatcgccg agcgcactaa cgcctcgcgc agcacggacc cggcgtccca cggttcggcg
    12961 cgcgcggcgt tctcgtccat catgcgtgcc agcagttcct ggtcgagcga gccccagttc
    13021 acgccgatgc gtatcggctt ctcgtgccgg caggccacct cgatcatccg cgcgaagttg
    13081 tcctcgcgct tcgcgccgcg accgacattg cccggattga tccggtacgc ggacaacgcc
    13141 tgcgcgcaag cggggaattc ggcgagcaac ttgtggccgt tgtagtggaa gtcgccgacg
    13201 agcggcacgt cgacgcccat ttggtcgagt tgtgcgcgga tcgccggcac ttcagccgac
    13261 gcttccgccg agttgacggt gatgcgaacc acctccgacc ccgccagaaa gagctccttg
    13321 acctggatcg cggtggcgat cgcgtcggcg gtgtcggtgt tcgtcatcga ctgcaccatc
    13381 accggcgcgt cgccgccgat gcggaccacg cgggcccccc agcgcacttg aacctgccgc
    13441 gttgcgcgcc ggggcgtggg cgcagcgcgc agcgacaggc agttccaggc ttcggtcggc
    13501 tgattcacgc gcgtccccta cttgaccatc agccgcgcga cattggcgac gccggtcacg
    13561 ggcttcaagt cgatcggctt gccctcgtac tcgagcgtga ccgacgacgc attgccgatc
    13621 acgatctggt acggcggctt gccgcgcacg atctgctcgc tgccggcagg attgttctgc
    13681 gccagcagca ccgccccgtc ctgctgtgtg acctcgaccc atgcaatgtc acgaaagccg
    13741 aagcgcagcg cgccaggctc gctcggcgcg gcggccggct gcgatgcagg tagcgacggt
    13801 gccgtaattt cagtcggcgc cggaccggcg gtgtcgccgg tcgtggttgc gctcggcgtc
    13861 gtggagggtg cagtcgcccg tgtcgcagtg tcggccacgg caggagcggc cgcctggcgc
    13921 gagcgggtgt tggcgatcca gccgatcacc gcgaacacga tcagcgcggc gatcgcacca
    13981 gcgatcacca gcgtgcgtga cccgcgttcc cgctcggccg tgcgcagcag cgtagcgcgc
    14041 acgggtgtct ctgcgctggc aggctcgagc atgcggttca gcgacgccag cagtggcgcc
    14101 ggatcgatgc gcacttcctt cgcgtagttg cgcacgatgc cgcgcacgaa tgccgtcccc
    14161 ggcaatgccg cgagatcttc gcgctcgagc gcgctcacct gcttcgcgct gatgcgcagc
    14221 cgggct
//
LOCUS       DBNHBY010000099         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_140815, whole genome shotgun sequence.
ACCESSION   DBNHBY010000099 DBNHBY010000000
VERSION     DBNHBY010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_140815"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC16HB_02440"
     CDS             <1..206
                     /locus_tag="AC16HB_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5605677.1"
                     /translation="DERARIDAAIRALPPALREVIVLREIEGLEYAEIARVASVPIGT
                     VMSRLSRAREKLRATLTQAGVGG"
     gene            208..912
                     /locus_tag="AC16HB_02445"
     CDS             208..912
                     /locus_tag="AC16HB_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5605678.1"
                     /translation="MTSDDDKLSALIREHATRYQASDQLRADVRTQIAIAAAGREQRT
                     PGFGRVGQSFGWLATAAGWLQGMGWRTASAGFALGVAAALLLTPVWRGLDLGDPLERQ
                     LVADHVRSLKVGPLTEVASSDRHTVKPWYQGRLDYAPTVFDLKEEGFPLVGGRIDHVR
                     GQSVAALTYMRRLHVMNVFEWPADGPQEPRHYERRGFNVVHWADGSMQYWIVSDVERG
                     EIERFVQAWRARAAAQ"
     gene            947..1426
                     /locus_tag="AC16HB_02450"
     CDS             947..1426
                     /locus_tag="AC16HB_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5605679.1"
                     /translation="MAHLTYPVAVVAAEAPARTKASNYPEPFASQMAGRSKQPLGDLF
                     GLTNFGVNLTRLAPNAVSSLRHAHSRQDEFVYILRGRPTLHTDAGRTRLAPGMCAGFK
                     AGTGNGHRLINDTQEDVVYLEIGDRTPGDEGTYPDDDLKAMLVDGKWKFVHKDGSPY"
     gene            1447..1776
                     /locus_tag="AC16HB_02455"
     CDS             1447..1776
                     /locus_tag="AC16HB_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HIR5605680.1"
                     /translation="MQISDIPFGTTDWSQVERTEHKGEQGVAYWRTRTFGAVRVRMVE
                     YSAGYRADHWCSKGHILLCLEGELHTSLKDGRSFVLRPGMSYQVADGAEPHRSWTTVG
                     ATLFIVD"
     gene            complement(1805..2194)
                     /locus_tag="AC16HB_02460"
     CDS             complement(1805..2194)
                     /locus_tag="AC16HB_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605681.1"
                     /translation="MHRLHIAGGLILGIAALIVAAHTRDSGGRPDRPIPGVVLFADGG
                     VHSIALSWSHNGASRYNVYLSSRPGCDIADYARCPDCRMFADVRSPLTIDALVNGRAY
                     YATVEGIHARGRSVSNEAAARPASLAR"
BASE COUNT          407 a          808 c          858 g          383 t
ORIGIN      
        1 gcgacgagcg tgcgcgcatc gacgccgcga tccgtgcgct gccgcccgcg ctgcgcgagg
       61 tgatcgtgct gcgcgagatc gaaggcctgg agtacgccga gatcgcgcgc gtggcgtcgg
      121 tgccgattgg cactgtaatg tcgcgcctgt cccgcgccag agagaagttg cgcgcgaccc
      181 tgactcaagc cggtgtgggt ggatagcatg acgagcgatg acgacaagct gtcggcgctg
      241 atccgcgaac acgcgacgcg ctaccaagcc agcgatcagc tgcgtgccga tgtgcgcacg
      301 cagatcgcga ttgccgccgc cggccgcgaa cagcggacgc cgggattcgg cagggtgggg
      361 caaagcttcg gctggcttgc cacggctgcc ggctggctgc agggaatggg ctggcgcacg
      421 gcatcggccg gattcgcgct cggcgttgcc gcggcgctgc tgctcacgcc ggtgtggcgc
      481 ggactggatc tcggcgatcc gctcgagagg caactcgtcg ccgatcacgt ccgctcgctg
      541 aaggtcggcc cgctgacgga agtcgcgtcg agcgatcggc acacggtcaa gccgtggtac
      601 cagggccggc tcgactacgc gccgaccgtg ttcgacctga aagaagaagg ctttcccctc
      661 gtcggcggac gcatcgatca cgtgcgtggc cagtcggtgg cggcgctgac gtacatgcgc
      721 cgactgcacg tgatgaacgt cttcgagtgg ccggccgacg gcccgcagga gccgcgccac
      781 tacgagcgtc gcggcttcaa tgtcgtgcac tgggccgacg gttcgatgca gtactggatc
      841 gtctccgacg tcgagcgcgg cgagatcgag cgcttcgtgc aggcctggcg cgcacgagcc
      901 gcggcgcagt gacgtcatac ttcacccatc ccgaagcgag cttcgcatgg cccatttaac
      961 ctatcccgtg gccgtcgtgg cggccgaagc gccggcaagg acgaaggcgt cgaactatcc
     1021 cgagccgttc gcgtcgcaga tggcgggtcg aagcaagcag ccgctcggcg acctgttcgg
     1081 ccttaccaac ttcggagtga atctgacgcg gctcgcgccg aacgcggtgt cgtcgctgcg
     1141 ccacgcgcac agcaggcagg acgaattcgt gtacatcctg cgtggacggc ccacgcttca
     1201 caccgacgcg ggccgcacgc ggctcgcgcc cggcatgtgc gccggcttca aggccggcac
     1261 cggcaatggc catcgattga tcaacgatac ccaggaagat gtcgtctacc tcgagatcgg
     1321 cgatcgaacg ccgggcgatg aaggtacgta tcccgacgac gacctgaagg cgatgctggt
     1381 cgacggcaag tggaagttcg tgcacaagga cggctcgccg tactgacacc tgcaaaggac
     1441 catcgcgtgc agatctccga catccctttc ggcaccaccg actggtcgca ggtcgagcgc
     1501 accgagcaca agggcgagca gggggtggcg tactggcgca cgcgaacctt cggcgccgtg
     1561 cgcgtccgca tggtcgagta cagcgcgggc tatcgcgccg atcactggtg cagcaagggg
     1621 cacatcctgc tgtgcctcga gggcgaactg cacacgagtc tcaaggacgg ccgcagcttc
     1681 gtgctccggc ccgggatgag ctaccaggtg gcggacggcg ccgagccgca ccgctcgtgg
     1741 accaccgtcg gcgcgacgct gttcatcgtc gactgagcgc gcgcctgccg cgcgtggtcc
     1801 gcattcagcg tgccagcgac gccggcctcg ccgcggcctc attcgaaacg ctgcgtcccc
     1861 gcgcgtggat gccttcgacg gtggcgtagt aggcgcggcc gttgaccagc gcatcgatcg
     1921 tcagcggcga gcgcacatcg gcaaacatgc ggcaatcggg gcagcgcgcg tagtccgcga
     1981 tgtcgcagcc cggacgcgac gacaggtaga cgttgtaacg ggacgcgcca ttgtggctcc
     2041 acgacagcgc gatgctgtgc acgccgccgt cggcgaacag caccacgccc gggatcggcc
     2101 ggtcgggtct gccgccgctg tcccgcgtgt gcgcagcgac gattagtgcg gcgatcccga
     2161 ggatcaatcc gccggcgatg tgaagacgat gcatggcatt gactccttga ttgcatgtgg
     2221 ttgagggcga tcggcgcgtt cctcggcgcc gacaggtcga cggcacgctg ttgaacgcgc
     2281 cgtcggctag cagcggcgcc ggcaccgatc gtgggaggcg acctgggcga agcggcgcgc
     2341 tgcagttatc gccggcgttt cggccgctga actcatgtcg gctccggcgc gtgccaacat
     2401 tcccgtgctg ccttcgagcg cgctgccgtc gcttcgaagg acgagaccat aagcgc
//
LOCUS       DBNHBY010000100         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_614403, whole genome shotgun sequence.
ACCESSION   DBNHBY010000100 DBNHBY010000000
VERSION     DBNHBY010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_614403"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1135
                     /locus_tag="AC16HB_02465"
     CDS             134..1135
                     /locus_tag="AC16HB_02465"
                     /inference="COORDINATES: protein motif:HMM:NF052859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5605682.1"
                     /translation="MVKEGDEVAAGAPIARLSLERSTASGATSSELVQRELTQRATNL
                     EAEKERVRQLAAQQREQIRTRVADLRKEIAQNDVEIRLQQTRVASAREDFQRTQQLVK
                     EGFVSDTALTQKRNDQLDQQVKLETLRRNRAGLERELRSAETELPTIDTRASQQIDLL
                     GRQKSELQQSIVQEEAKRETIIRAPIAGVVTNIAVSRGASVAADAPIAMLLPKGSGLQ
                     AQLLVPTRAIGFVQPGNDVVLRYEAFPFQRFGQYHGKVEAISRTVWSTGEKVGSMSAR
                     EPVYRIDVKLDRQSVAAGNQEFPLRPGMLVNADILLERRTVFEWVFEPVLQLKERLS"
     gene            1135..>2878
                     /locus_tag="AC16HB_02470"
     CDS             1135..>2878
                     /locus_tag="AC16HB_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR5605683.1"
                     /translation="MFGRRVPLILQSEAPECGIACVAMIASHHGYLTDLSAMRMRLAP
                     SMRGVTLQHIAAIAQTMNMSARGVQAPLEALGQLRLPAILHWDMNHFVVLTKVAGQRI
                     TVHDPARGKRVLTLGEASRHYTGVAMELQPAAGFAKRDEREKISAWQLLSAASGMKNA
                     IVQVLLLSVVLEVFAIASPFFVQVVVDRVVVGRDLDLLVVLGLGFALLVVMQVAVTAV
                     RAWLGVYLSTNLNLRLLDTLFGQLLKLPLAWFEKRQLGDIFARFKSVDAIQKTLTLTF
                     VETIVDGAMVILTLAVMFWYSTLLSSIVLAAAILYGLVRWAFYGPQRRALDEQIVHEG
                     KSNTHFIETLRGMLAIKLNLRENERRSAYQNLVVDHTNAGVKAQNIAIVHRAANGLIF
                     GLENVAVIWLAALSVIEGQFSVGMLFGFLAFKLLFLTRVNNLVDKAIDFRMLDLHAER
                     IADIALATPEPVRTGAAEPAGTPLTIEGKQLSFAYGVEGFVFRGVDFALHPGETVAIV
                     GPSGSGKTTLVKVLVGLLDRSEGSLRIAGRDARDWDHAAYRGRIGVVMQDDQLFVGTI
                     EDNISFFDPQHDAER"
BASE COUNT          482 a          945 c         1004 g          447 t
ORIGIN      
        1 gatcggaaga gcacacgtct gaactccagt cctcggcgaa tacacgcggc gcgaacgcgt
       61 cgaaggattt ctcgcgctcg acgcgggcgc ggcccgcatc caggcgccgg aaatcggcac
      121 catcagcgac ctgatggtca aggaaggcga cgaggttgcg gccggtgcgc cgatcgcacg
      181 gctgtcgctc gagcgcagca cggcgtccgg cgccacgtcg tcggaactcg tgcagcgcga
      241 actgacgcag cgcgcgacca acctggaagc ggagaaggag cgcgtgcgac agctcgccgc
      301 gcagcagcgc gagcagatcc gcacgcgtgt cgccgacctg cgcaaggaga tcgcgcagaa
      361 cgacgtcgag atccggctgc agcagacgcg cgtggcctcg gcccgcgaag acttccagcg
      421 cacgcagcag ctggtgaagg aaggcttcgt gtccgacacc gcgctgacgc agaagcgcaa
      481 cgaccagctc gaccagcagg tcaagctcga aaccctgcga cgcaatcgcg ccgggctgga
      541 gcgcgagctg cgttctgccg agaccgaact gccgacaatc gacacgcgcg ccagccagca
      601 gatcgacctg ctcggccggc agaagagcga gctgcagcag agcatcgtcc aggaggaagc
      661 caagcgcgag acgatcatcc gcgcgccgat cgccggcgtg gtgaccaaca tcgcggtgtc
      721 gcgcggcgcc tcggtcgccg ccgacgcccc gatcgcaatg ctgctgccga agggcagcgg
      781 attgcaggcg cagctgctgg tgccgacgcg cgcgatcggc ttcgtgcagc cgggcaacga
      841 cgtggtgctg cgctacgagg cgtttccgtt ccagcgcttc ggccagtatc acggcaaggt
      901 cgaggcgatc agtcgcaccg tgtggtcgac cggcgaaaag gtgggatcga tgagcgccag
      961 ggagccggtg taccggatcg acgtgaagct cgaccggcaa tcggtggccg ccggcaacca
     1021 ggagttcccg ttgcggccgg gcatgctggt caatgccgac atcctgctcg agcgccgcac
     1081 cgtgttcgag tgggtgttcg agccggtgct gcaactgaag gaaagactga gctagtgttc
     1141 ggccgccgcg tcccgctgat cctgcagtcc gaggcgccgg agtgcggcat cgcctgcgtg
     1201 gcgatgatcg ccagccacca cggctacctg accgatctgt cggcgatgcg catgcggctg
     1261 gcgccgtcga tgcgcggcgt cacgctgcag cacatcgccg cgatcgcgca gacgatgaac
     1321 atgtcggcgc gtggcgtgca ggcgccgctg gaagcgctcg gccagctgcg tctgccggcg
     1381 atcctgcact gggacatgaa ccacttcgtc gtgctgacga aggtcgcggg ccagcgcatc
     1441 accgtgcacg acccggcgcg cggcaagcgc gtgctgacgc tcggcgaggc gtcgcgccac
     1501 tacaccgggg tggcgatgga actgcagccg gccgccggct tcgccaagcg cgacgagcgc
     1561 gagaagatca gcgcctggca gctgctgtcg gcggcctcgg gcatgaagaa cgcgatcgtg
     1621 caggtgctgc tgctgtcggt cgtgctcgaa gttttcgcga tcgcctcgcc gttcttcgtg
     1681 caggtcgtcg tcgaccgcgt ggtggtcggg cgcgatctcg atctgctggt cgtgctcggg
     1741 ctgggcttcg cgctgctggt cgtgatgcag gtcgcggtca ccgcggtgcg cgcgtggctg
     1801 ggcgtgtacc tgtcgacgaa cctgaacctg cgcttgctcg acaccctgtt cgggcagttg
     1861 ctgaagctgc cgctggcatg gttcgagaag cgccagctcg gcgacatctt cgcgcgcttc
     1921 aagagcgtgg acgcgatcca gaagacgctg accctgacct tcgtcgagac catcgtcgac
     1981 ggcgcgatgg tcatcctcac cctggcggtg atgttctggt acagcacgct gctgtcgtcg
     2041 atcgtgctgg cggcggcgat cctgtacggc ctggtgcgct gggcgttcta cggcccgcag
     2101 cgacgcgcgc tggacgagca gatcgtgcac gagggcaagt cgaacacgca cttcatcgag
     2161 acgctgcgcg gcatgctggc gatcaagctc aacctgcgcg agaacgagcg ccgctccgcc
     2221 taccagaatc tcgtcgtcga ccacaccaac gccggcgtga aggcgcagaa catcgcgatc
     2281 gtgcaccgcg ccgccaacgg gctgatcttc gggctggaga acgtcgccgt gatctggctg
     2341 gcagcgctct cggtgatcga ggggcagttc tcggtcggca tgcttttcgg gttcctcgcg
     2401 ttcaagctgc tgttcctgac gcgggtgaac aacctggtcg ataaggccat cgacttccgg
     2461 atgctcgatc tgcacgccga gcgcatcgcg gatatcgcgc tcgccacgcc cgagccggtg
     2521 cgcacgggcg ccgcggagcc cgccggcacg ccgctgacga tcgagggcaa gcaactgagc
     2581 ttcgcctacg gcgtcgaagg cttcgtgttc cgcggcgtcg acttcgcgct gcacccgggc
     2641 gagacggtgg ccatcgtcgg tccctccggc tccggcaaga cgaccctggt caaggtgctg
     2701 gtggggctgc tcgaccggag cgaaggctcg ttgcgcatcg ccggacgcga tgcgcgcgac
     2761 tgggaccacg ccgcgtaccg cggcaggatc ggcgtcgtga tgcaggacga ccagctgttc
     2821 gtcggcacga tcgaggacaa catcagcttc ttcgatccgc agcacgacgc cgaacgcg
//
LOCUS       DBNHBY010000101         4563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1832766, whole genome shotgun sequence.
ACCESSION   DBNHBY010000101 DBNHBY010000000
VERSION     DBNHBY010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1832766"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16HB_02475"
     CDS             <1..218
                     /locus_tag="AC16HB_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605684.1"
                     /translation="GGAVRGTVSLTHGFCAGEYPVKGELDGNKLTLRHSVKGEGKQGC
                     GVNCELTVAGNKLEGKTKSGSLIRFSK"
     gene            complement(265..1023)
                     /locus_tag="AC16HB_02480"
     CDS             complement(265..1023)
                     /locus_tag="AC16HB_02480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5605685.1"
                     /translation="MRLAGKVAIVTGAGSGFGEGIAQRFAQEGCAVIVNDLAREAGER
                     VASAIRSAGGRAQFAYGDVSRGEDVKALLDAALKHFGALDIVVNNAGTTHRNQPLLAV
                     SEAEFDRVYAVNVKSLYWTAMHMVPYFRTRGGGCFVTIASTAGVRPRPGLVWYNGSKG
                     AAIVASKAMAAELGPDNIRVNCVNPVIGETGLLEQFMGMPDTPENRKRFLATIPLGRF
                     SKPLDIANACVFLASDEAAFITGACLEVDGGRCV"
     gene            complement(1036..2487)
                     /locus_tag="AC16HB_02485"
     CDS             complement(1036..2487)
                     /locus_tag="AC16HB_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5605686.1"
                     /translation="MEGKHHFIGNRWVGAQSGQSIDVVDPSDGQPFAKIARGNAADID
                     AAVRAARAAFGEFGSGDWGRCTATERGRLLAKLSARVAAQVDELARLESRTTGKPLKQ
                     GRADANALARYFEYYGGAADKLHGETVPYSNGMTVLTIREPHGVTGHIIPWNYPLQIF
                     GRSVGAALAAGNACVVKPAEDACLSLARVAELAAEVGFPDGALNIVTGYGNEAGAALA
                     AHGGIDHISFTGSPVTGTAVAQAAAKHHCPVTLELGGKSPQIVFADAHLDDVVPVVVN
                     AIVQNAGQTCSAGSRLLVERAIYEELLARVGDVMSRLKVGPALADLDVGPLIRRSQLQ
                     RVWDYLSQAQVDKIPVAAQGHVVEEAPQTGFYQAPTLLRDVPPTHKLAQEEIFGPVLA
                     ALPFDDEDEALRIANGTLFGLVAGVWTRDGARALRVARKVRSGQVFINNYGAGGGIEL
                     PFSGVKSSGYGREKGFEALLGFTTLKTIAIKHG"
     gene            2593..3141
                     /gene="ppa"
                     /locus_tag="AC16HB_02490"
     CDS             2593..3141
                     /gene="ppa"
                     /locus_tag="AC16HB_02490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR5605687.1"
                     /translation="MSLDRVPSGRALPDDFNVVIEIPAHADPVKYEVDKESGALFVDR
                     FIGTSMHYPCNYGYIPHTISDDGDPVDVLVITPFPLQHGVVVRCRPLGVLQMHDEAGG
                     DSKLLAVPIDKCLPWYKHWRKPEDIAPERLAQIQHFFEHYKDLEPGKWVKITGWAGPD
                     EARREIMSAVDAFKQASPKPAF"
     gene            complement(3110..3862)
                     /locus_tag="AC16HB_02495"
     CDS             complement(3110..3862)
                     /locus_tag="AC16HB_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-binding di-iron domain-containing
                     protein"
                     /protein_id="HIR5605688.1"
                     /translation="MITNPQSRTNVHEIADGIYRINTPVDIPDGPCGFSFNQYLIVDD
                     APLLFHTGPRRMFPLVREAVARLIPVEQLRYVAFSHVEADECGALNDWLASVPRAQPV
                     CSRIAAMVSINDIADRAPRALADGETLTLGRHTVRWFDTPHVPHGWDCGLMMEERTRT
                     FLCGDLFTQGGAGELALTESDILEPSEAFRAPLDYFAHAPATDAILERLAKEAPTTLA
                     CMHGSAWRGDGARLLRALAGRLVRTPASAMPA"
     gene            3962..4531
                     /locus_tag="AC16HB_02500"
     CDS             3962..4531
                     /locus_tag="AC16HB_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5605689.1"
                     /translation="MPSQVPPAIDDATLARRIAAAHPATDAGAEAELCRRFAPRVRLY
                     GLRHLRDAHAASDLAQQVLMLTLEALRNGKLRDPEQLVSFLFGTCRQVVVDQRRTQAR
                     RDDLLATYAGDVPIADPSGGPRLDHARLGQCLQRLAERERSVLVLTFYDDRAAKDVAA
                     ELALTEANVRVIRHRALQRLRECVTGGAS"
BASE COUNT          709 a         1553 c         1556 g          745 t
ORIGIN      
        1 agggtggggc ggtgcggggc actgtatcgc tgacccacgg attctgcgca ggcgagtacc
       61 cggtcaaagg cgaactggac ggcaacaagc tgactttgcg ccacagcgta aagggagaag
      121 ggaagcaggg ttgcggcgtc aactgtgaat tgacggtggc cggcaacaag ctcgaaggca
      181 aaacgaagtc cggaagcttg atacgtttct cgaaatagcg cccgcgctgc gggcgctcga
      241 gcatgggtat tgccatgacg tcgatcagac gcagcgcccg ccgtcgactt ccaggcatgc
      301 gccggtgatg aaggcggcct cgtcggaggc gaggaacacg caggcgttgg cgatgtcgag
      361 cggcttgctg aatcgaccga gcggaatcgt ggcgaggaac cgcttgcggt tctccggcgt
      421 gtcgggcatg cccatgaact gttcgagcag cccggtctcg ccgatcaccg ggttcacgca
      481 gttgacccgg atgttgtccg ggccgagttc ggcggccatc gccttgctgg cgacgatagc
      541 ggcgcccttg ctgccgttgt accagacgag ccccggccgc ggccgcacgc cggcggtcga
      601 cgcgatcgtc acgaagcagc cgccgccgcg cgtgcggaaa tacggcacca tgtgcatcgc
      661 ggtccagtac aggctcttca cgttgaccgc gtagacgcgg tcgaattccg cttcgctgac
      721 ggcgagcagc ggctgattgc gatgcgtggt gccggcgttg ttgacgacga tgtcgagcgc
      781 gccgaagtgc ttcagcgcgg catcgagcag cgctttcacg tcctcgccgc gcgacacgtc
      841 gccgtacgcg aactgagcgc ggccgccggc gctgcgaatc gcgctcgcca cgcgctcccc
      901 ggcctcgcgc gccagatcgt tgacgatcac tgcacagcct tcctgcgcga agcgttgcgc
      961 gatgccctcg ccaaaacccg agcccgcgcc ggtgacgatc gcgaccttgc ctgccagacg
     1021 cattcctctc tcctctcagc cgtgtttgat cgcaatcgtc ttcagcgtcg tgaacccgag
     1081 cagcgcctcg aacccctttt cacgcccgta gccgctcgac ttcacgccgc tgaacggcaa
     1141 ctcgatgccg ccgccggcac cgtagttgtt gatgaacacc tggccgctcc tcaccttgcg
     1201 cgccacgcgc agcgcacgcg caccgtcgcg cgtccacacg cccgcgacca acccgaacag
     1261 tgtgccgttg gcgatgcgca gcgcctcgtc ctcatcgtcg aacggcagtg cggcgagcac
     1321 cgggccgaag atctcctcct gcgccagctt gtgcgtcggc gggacgtcgc gcagcaatgt
     1381 cggcgcctgg tagaagcccg tctgcggcgc ctcttcgacc acgtggccct gtgctgcgac
     1441 cgggatcttg tcgacctgcg cctgcgacag gtaatcccac acgcgctgca gttgcgagcg
     1501 gcggatcagc gggccgacgt cgaggtcggc cagcgcaggc ccgaccttca accgcgacat
     1561 cacgtcgccg acgcgcgcga gcagttcttc gtagatcgcg cgctcgacca gcagccggct
     1621 gccggccgag cacgtttgcc ccgcgttctg cacgatcgcg ttgacgacca ccggcaccac
     1681 gtcgtcgaga tgggcgtcgg cgaacacgat ctgcggcgac ttgccgccaa gctcgagcgt
     1741 gaccgggcag tggtgcttcg ccgccgcctg cgcgaccgcg gtgccggtca ccggcgagcc
     1801 ggtgaacgag atgtgatcga tgccgccgtg cgcggcaagt gccgcgccgg cctcgttgcc
     1861 gtagccggtg acgatgttca gcgcgccgtc ggggaatccg acctcggccg ccagttctgc
     1921 gacgcgcgcg agcgacaggc acgcatcctc ggcgggcttg accacgcacg cgttgccggc
     1981 ggcgagcgcg gcaccgacac tgcgtccgaa gatctgcagc ggatagttcc acggaatgat
     2041 gtggcccgtc acgccgtggg gctcgcgaat cgtcagcacc gtcatgccgt tgctgtacgg
     2101 caccgtctcg ccgtgcagct tgtcggctgc gccgccgtag tactcgaagt agcgcgccag
     2161 cgcgttcgca tcggcgcggc cctgtttcaa cggcttgccg gtcgtgcgcg attcgagccg
     2221 cgccagctca tcgacctgcg ccgccactcg cgcggagagc ttcgcgagca atcgcccgcg
     2281 ctcggtcgcg gtgcagcggc cccagtcgcc gctgccgaat tcgccgaacg ccgcgcgtgc
     2341 cgcacggacc gcggcgtcga tgtcggccgc attgccgcgc gcgatcttgg cgaacggctg
     2401 gccgtcggat ggatcgacga cgtcgatcga ctgccccgac tgcgccccga cccagcgatt
     2461 gccgatgaag tgatgcttgc cctcgatggg cgtccagccg ccatccatgg tcgtctcctc
     2521 gcgtcgatcg acgattatcg gccagtgcgt tgccgctcgc tagaatcgcc gctacttccc
     2581 tgaggaaccg ccatgagcct ggatcgtgtg ccatcgggcc gcgcgctgcc ggacgacttc
     2641 aacgtcgtga tcgagattcc cgcgcatgca gatcccgtca agtacgaagt cgacaaggag
     2701 tcgggcgcgc tgttcgtcga ccgtttcatc ggcacgtcga tgcactaccc gtgcaactac
     2761 ggttacatcc cgcacacgat ctccgacgac ggcgatccgg tcgacgtgct cgtgatcacg
     2821 ccgtttccgc tgcagcacgg cgtggtggtg cgctgccgcc cgctcggcgt gctgcagatg
     2881 cacgacgagg cgggcggcga ttcgaagctg ctcgcggtgc cgatcgacaa gtgcctgccc
     2941 tggtacaagc actggcgcaa gcccgaggac atcgcgcccg agcggctggc gcagatccag
     3001 cacttcttcg agcactacaa ggatctcgag cccggcaagt gggtgaagat caccggctgg
     3061 gctggccccg acgaggctcg gcgcgagatc atgtcagcgg tcgatgcgtt caagcaggca
     3121 tcgccgaagc cggcgttctg acgagccgcc ccgcgagcgc gcggagaagc cgcgcgccgt
     3181 cgccgcgcca tgcggaaccg tgcatgcacg cgagcgtggt cggagcttcc ttcgcgagcc
     3241 gttcgaggat cgcatcggtc gcgggtgcat gcgcgaagta gtcgagcggc gcgcggaacg
     3301 cctcgctcgg ctcgaggatg tcggactcgg tcagcgcaag ctcgccggcg ccgccctgcg
     3361 tgaatagatc gccgcacagg aacgtgcgcg tgcgctcctc catcatcagc ccgcagtccc
     3421 agccgtgcgg cacgtgtggc gtgtcgaacc accgcactgt gtggcgtccg agcgtcagag
     3481 tctcgccgtc ggcgagcgcg cgcggcgcac ggtcggcgat gtcgttgatc gacaccatcg
     3541 cggcgatccg gctgcacacc ggctgcgcgc gcggtacgga tgcgagccaa tcgttcagcg
     3601 cgccgcattc gtccgcctcg acgtgcgaga aggcgacgta gcgcaactgc tcgaccggga
     3661 tcagccgggc gacggcctcg cgcacgagcg gaaacatgcg gcgcggcccc gtgtggaaca
     3721 gcagtggcgc gtcgtcgacg atcagatact ggttgaagct gaagccgcag ggtccgtccg
     3781 gaatgtcgac cggggtgttg attcgataga tgccatcggc gatttcgtgg acgttggtgc
     3841 gggactgcgg gttcgtgatc atgggacgct cctgttgggg gttgcggacg aggttgtgta
     3901 acgaaccgcc gtttcgttac acctcctcgt tcgattgccg caggtcgcct atgcttgccg
     3961 cgtgccgagc caagtaccac ccgcgatcga cgacgcgacg ctggcgcgcc gcatcgctgc
     4021 cgcgcatccg gcgaccgatg ccggcgcgga ggccgaactg tgccgccgct tcgcgccgcg
     4081 cgtgcgcctg tacgggttgc ggcatctgcg cgacgcgcac gcggcgtcgg acctggcgca
     4141 gcaggtgctg atgctgacgc tcgaagcgct gcgcaacggc aagctgcgcg atccagagca
     4201 actggtgtcg ttcctgttcg gcacgtgtcg ccaggtcgtg gtcgaccagc ggcgcacgca
     4261 ggcgcggcgc gacgacctgc tcgcgaccta tgcgggcgac gtgccgatcg ccgatccgtc
     4321 gggcgggcca cgtctcgacc acgcgcggct cgggcagtgc ctgcagcggc tggccgagcg
     4381 cgagcgttcg gtgctggtgc tgacgttcta cgacgatcgc gcggcaaagg acgtggcggc
     4441 ggaactggcg ctgaccgagg cgaacgtgcg cgtgatccgc caccgcgcgc tgcagcggct
     4501 gcgcgagtgc gtgaccggag gagcctcatg agctgtgccg caccgatcgc atcgcggacg
     4561 ttg
//
LOCUS       DBNHBY010000102         5984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_817240, whole genome shotgun sequence.
ACCESSION   DBNHBY010000102 DBNHBY010000000
VERSION     DBNHBY010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_817240"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="AC16HB_02505"
     CDS             complement(<1..1357)
                     /locus_tag="AC16HB_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR5605690.1"
                     /translation="MGKVTGFLEYARLEEAAEAPASRKKHWREFVTHLTDEQASIQGA
                     RCMDCGIPFCSNGCPVNNVIPDFNDLVYRQDFRTALDVLHSTNNFPEFTGRVCPAPCE
                     AACTLNINTDPVGIKSIEHKIIDKGWEEGWVEPQPPARRSGRKVAIVGSGPAGMAAAQ
                     QLARAGHDVTVLEKNDRIGGLLRYGIPDFKMEKSHIDRRIAQMEAEGVTFRTGVLVGK
                     GKLNGGVVDWSRQTIDPDQLLKDFDAVVLAGGAEQPRDLPVPGRELNGVHFAMEFLPQ
                     QNKVVAGDKVKDQITAAGKRVLVIGGGDTGSDCVGTSNRHGAKSVTQWELLPQPPKSE
                     NKELTWPYWPLKLRTSSSHEEGVERDWSITTKRFNGDGKGNVKSLTAARIEWHKDQTT
                     GQMKMVEVGSEFDVPADLVLFAMGFVSPVAAVLDAFGVEKDPRGNARATTDGSGSYRT
                     SV"
     gene            complement(1365..>5984)
                     /locus_tag="AC16HB_02510"
     CDS             complement(1365..>5984)
                     /locus_tag="AC16HB_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR5605691.1"
                     /translation="FVANIKGKASHSLIQQALLILENLDHRGAVGADPLCGDGAGILI
                     QIPDAFYRREMVKLGVALPPPGEYGVGMIFLPRENASRLACEQELERTVRAEGQVVLG
                     WRDVPVDRDMPMSPFVRDKEPMIRQIFIGRGPDVMVPAALERKLYVIRKTASHKIQAL
                     KLQHGKEYFVPSMSARTVVYKGLLLADQVGRYYRDLREPEVVSAIALVHQRFSTNTFP
                     SWELAHPYRMIAHNGEINTVKGNFNWIRAREGVMASPVLKDDLKKLWPLIYAGQSDTA
                     CFDNCLELLTMAGYPLAHAVMMMIPEAWEQHTLMDENRRAFYEYHAAMMEPWDGPAAI
                     AFTDGVRIGATLDRNGLRPARYVVTDDDIVVMASEVGVLPIPESRIVKKWRLQPGKMF
                     LIDTEQGRIIDDAEIKEQLAKSQPYKEWIRRIAIKLDALAPEGEAKSAARLDVPALDR
                     QQAFGYTQEDLKLLMAPMAEAGEEAIGSMGNDSALPVLSDRAKPLYSYFKQLFAQVTN
                     PPIDPIREQLVMSLVSFVGPKPNLLDINNINPPMRLEVSQPVLDFKDMAKIRGIEQYA
                     SGKFRSYEIDICYPVAWGKEGVEARLASLCAEAVDAVRAGYNILIVTDRKLDARHVAM
                     PALLATSALHQHLIDAGLRTATGLVVETGSAREVHHFALLAGYGAESIHPYLAMETLA
                     MQYAGKPGGTDADKAIKNYIKAIGKGLQKVMSKMGISTYMSYCGAQIFEAVGLNRGLV
                     DKYFRGTASNIEGIGLFEVMEEAVRMHRAAFGNDPVLANMLDAGGDYQWRTRGEEHMW
                     TPDAIAKLQHSTRANSYQTYKEYAQIINDQSRRRMTLRGLFEFKVDHRRAIQLSEVEP
                     AKDIVKRFATGAMSLGSISTEAHSTLAIAMNRIGGKSNTGEGGEDPRRYAQELRGIAI
                     KRGDTLASVLGSDRVEVDLPLQEGDSLRSKIKQVASGRFGVTTEYLVSADQIQIKMAQ
                     GAKPGEGGQLPGHKVSAYIAELRFSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANP
                     RASISVKLVSEVGVGTVAAGVSKAKADHVTIAGHDGGTGASPVSSIKHAGTPWELGLA
                     ETQQTLVLNRLRGRIAVQVDGQMKTGRDVVIGALLGADEFGFATAPLVVEGCIMMRKC
                     HLNTCPVGVATQDPVLRRRFKGQPEHVVNYFFFVAEEVREIMAQLGIRKFDDLIGRTD
                     LLDIKRGIDHWKAKGLDFSRIFYMPPMPADVARYRCEEQDHGLARALDLKLIEQSRPA
                     LDKRDRVSFISPIRNVNRTVGTMLSSEVARRFGHAGLSDDTIHIQFNGTAGQSFGAFL
                     AAGITLDLVGDANDYTGKGLSGGRIIVRSPNDFRGFGPEHIIVGNTVLYGAIAGEAYF
                     NGVAGERFAVRNSGASAVVEGTGDHGCEYMTKGTVVVLGGTGRNFAAGMSGGIAYVYD
                     EHGDFAKRCNTSMVTLEPVLTTQEQQEKVDEAIWHLGETDEFILRDLLEKQFRYTGSF
                     RAKELLHNWPAMRTRFVKVFPHEYRRALKQMHEAQRKVELQKLAA"
BASE COUNT          917 a         2017 c         1893 g         1157 t
ORIGIN      
        1 ccacgctggt ccggtagctg ccgctgccgt cggtcgtcgc gcgggcgttt ccgcgcggat
       61 ccttctcgac gccgaatgcg tcgagcaccg cggccaccgg cgacacgaag cccatcgcga
      121 acagcacgag gtcggcggga acgtcgaatt cggagccgac ctcgaccatc ttcatctggc
      181 cggtcgtctg gtccttgtgc cactcgatgc gagcggctgt cagcgacttg acattgccct
      241 tgccgtcgcc gttgaagcgc ttcgtcgtga tcgaccagtc gcgctcgaca ccttcttcgt
      301 gcgagctgga cgtgcgcagt ttcagcggcc agtacggcca cgtcagttcc ttgttctcac
      361 tcttcggcgg ctgcggcaac agctcccact gcgtaacgct cttggcgcca tggcggttgc
      421 tggtcccgac gcagtcggag ccggtgtcac cgccgccgat caccagaacg cgcttgccgg
      481 ccgcagtaat ctgatccttg accttgtcgc ccgcgaccac cttgttctgc tggggcagga
      541 actccatcgc aaagtggacg ccgttgagtt cccgccccgg caccgggaga tcgcgcggct
      601 gctcggcacc gccggcgagc acgaccgcgt cgaagtcctt cagcagctgg tcagggtcga
      661 tcgtctgcct cgaccagtcg accacgccgc cattgagctt gccctttccg accagcacgc
      721 cggtgcggaa cgtcacgccc tccgcctcca tctgcgcgat gcggcggtcg atgtgcgatt
      781 tctccatctt gaagtcgggg atgccatagc gcagcagccc gccgatgcgg tcgttcttct
      841 ccaacaccgt gacgtcatgg ccagcgcgcg caagctgctg cgcggcggcc atacccgccg
      901 ggccgctgcc gacgatcgcg accttcctgc cggacctgcg cgccggcggc tgcggctcga
      961 cccagccttc ctcccagccc ttgtcgatga tcttgtgctc gatcgacttg atgccgaccg
     1021 gatcggtgtt gatgttcagc gtgcatgcgg cctcgcacgg cgccggacac acgcgtccgg
     1081 tgaactcggg aaagttgttc gtgctgtgga gcacgtcgag cgcagtcctg aagtcctgcc
     1141 ggtagacgag gtcgttgaag tccggaatga cgttgttgac cgggcagccg ttgctgcaga
     1201 agggaatacc gcagtccatg cagcgcgcac cctggatcga cgcctgctcg tccgtcaggt
     1261 gcgtgacgaa ctcgcgccag tgcttcttgc gcgacgcggg cgcttcggcg gcttcttcca
     1321 gccgcgcgta ttcgaggaat ccggtgactt ttcccatttc gacctcacgc cgcaagcttc
     1381 tgcagctcga ccttgcgctg tgcttcgtgc atctgtttca gggcgcgccg gtactcgtgc
     1441 gggaagacct tgacgaacct ggtgcgcatc gccggccagt tgtgcagcag ctccttggcg
     1501 cggaagctgc cggtgtagcg gaattgcttc tccagcagat cgcgcaggat gaactcgtcg
     1561 gtctcgccga ggtgccagat cgcctcgtcg actttctcct gctgctcctg tgtcgtcagc
     1621 accggctcga gcgtcaccat cgacgtgttg cacctcttcg cgaagtcgcc gtgctcgtcg
     1681 tagacgtaag cgatgccacc tgacatgccg gccgcgaaat tgcgccccgt cccgcccagc
     1741 acgacgacgg tgccctttgt catgtactcg cagccatggt cgcccgtacc ctcgaccacc
     1801 gcgctcgcac cggagttccg gaccgcaaag cgctcgcccg ccacgccgtt gaaatacgcc
     1861 tcgccggcaa tcgcgccgta cagcacggtg ttgccgacga tgatgtgctc ggggccgaaa
     1921 ccacggaagt cgttcgggct gcgcacgatg atgcggccgc ctgacagccc cttgccggtg
     1981 tagtcgttgg cgtcgccgac gaggtccagc gtgatacctg cggccaggaa cgcaccgaag
     2041 ctctgtcccg cggtgccgtt gaactggatg tggatcgtgt cgtcggacag tcctgcgtgc
     2101 ccgaagcggc gcgcgacctc ggacgacagc atcgtgccaa ctgtccggtt gacgttgcgg
     2161 atcggcgaga tgaagctgac gcggtcgcgt ttgtccagcg ccggccggct ttgctcgatc
     2221 agcttcaggt ccagcgctcg cgccagtccg tgatcctgtt cctcgcacct gtaacgcgcc
     2281 acgtcggccg gcattggcgg catgtagaaa atgcgcgaga agtcgagccc cttcgccttc
     2341 cagtggtcga tgccgcgttt tatgtcgagc aggtcggtgc ggccgatcag gtcgtcgaac
     2401 ttgcggatgc caagctgcgc catgatctcg cgcacctctt ccgcgacgaa gaagaagtag
     2461 ttgacgacgt gttcgggctg gcccttgaag cggcggcgca gcaccgggtc ctgcgtggcc
     2521 acgccgaccg ggcaggtgtt caggtggcac ttgcgcatca tgatgcagcc ctcgaccacg
     2581 agcggcgcgg tggcgaagcc gaactcgtcg gcacccagca gcgcgccgat cacgacgtcg
     2641 cgtccggtct tcatctggcc gtcgacctgc actgcgatgc ggccgcgcag gcgattgagc
     2701 accagcgtct gctgcgtctc cgcgagcccc agctcccatg gtgtgcccgc gtgcttgatg
     2761 gacgacaccg gtgacgcgcc ggtgccgccg tcgtggccgg cgatcgtcac gtgatcggcc
     2821 ttcgccttcg acacgcctgc ggcgacggtg ccgacgccga cctcgctgac cagcttcacg
     2881 ctgatcgacg cgcgcggatt ggcgttcttc agatcgtgga tcagctgcgc caggtcctcg
     2941 atcgaataga tgtcgtggtg cggtggaggc gaaatcagac ccacgccggg gaccgagaag
     3001 cgcagctccg cgatgtaggc gctgaccttg tggcccggga gctgaccccc ttcgccgggc
     3061 ttcgcaccct gcgccatctt gatctggatc tggtcggcgc tgactaggta ctcggtcgtg
     3121 accccgaacc ggcccgaggc gacctgtttg atcttcgatc gcaacgagtc gccttcctgc
     3181 agcggtagat ccacttccac gcgatcgctg cccagcacgc tcgccagcgt gtcgccgcgc
     3241 ttgatcgcaa tgccgcgcaa ctcctgcgca taacggcgcg ggtcttcgcc gccctcgccc
     3301 gtgttgctct tgccgccgat gcggttcatc gcgatcgcga gcgtggagtg cgcctcggtc
     3361 gagatcgagc cgagcgacat cgcgccggtg gcaaagcgct tgacgatgtc ctttgccggt
     3421 tcgacctccg aaagctggat cgcacgccgg tgatcgacct tgaactcgaa gagcccgcgc
     3481 agcgtcatgc gccgccggct ctggtcgttg atgatctgcg cgtattcctt gtaggtctgg
     3541 tacgagttgg cgcgtgtcga atgctgcagc ttggcgatcg catcgggcgt ccacatgtgc
     3601 tcctcgccgc gggtgcgcca ctggtagtcg ccgccggcgt cgagcatgtt cgcgagtacc
     3661 ggatcgttgc cgaacgcggc ccggtgcatg cgcaccgcct cttccatcac ttcgaacagg
     3721 ccgatgccct cgatgttgct cgcggtgccg cgaaagtact tgtcgaccag gccgcggttc
     3781 aggcctaccg cctcgaagat ctgcgccccg cagtacgaca tgtaggtcga gatgcccatc
     3841 ttggacatca ccttctgcag ccccttgccg atcgccttga tgtagttctt gatcgccttg
     3901 tcggcgtcgg tgccgccggg cttgcccgca tactgcatcg cgagcgtttc catcgcgagg
     3961 tacgggtgga tcgactcggc gccgtagccg gcgagcagcg cgaagtggtg cacttcgcgc
     4021 gccgagccgg tctcgaccac gagtccggtc gcggtgcgca gtcctgcgtc gatcagatgc
     4081 tgatgcaatg cgctcgtggc aagcagcgcg ggcatcgcga cgtgccgggc atcgagcttg
     4141 cggtcggtaa cgatcaggat gttgtagccg gcgcgcaccg cgtcgaccgc ctcggcgcac
     4201 agcgacgcca gccgcgcctc cacgccctcc ttgccccagg cgaccggata gcagatgtcg
     4261 atttcgtagc tgcggaactt gcccgatgcg tactgttcga tgccccggat cttcgccatg
     4321 tccttgaagt cgagcaccgg ttggctgacc tccaggcgca tcggcggatt gatgttgttg
     4381 atgtcgagca ggttgggctt cgggccgacg aagctcacca gcgacatcac gagctgctcg
     4441 cggatcggat cgatcggcgg gttggtgacc tgggcgaaca gctgcttgaa gtaactgtaa
     4501 agaggcttgg cgcggtcgga cagcacgggc agcgcgctgt cgttgcccat cgacccgatt
     4561 gcctcctcgc ccgcctcggc catcggcgcc atcagcagct tcagatcctc ctgcgtgtag
     4621 ccgaacgcct gctggcggtc gagcgccggc acgtcgagtc gcgccgccga tttcgcctcg
     4681 ccttccggcg ccagcgcatc gagcttgatc gcgatgcgcc ggatccactc cttgtatggc
     4741 tggctcttgg cgagctgttc cttgatctcg gcgtcatcga tgatgcggcc ctgctcggta
     4801 tcgatcagga acatcttgcc gggctgcagc cgccacttct tgacgatcct gctttctggg
     4861 atcggcagca cgccgacctc ggaggccatc acgacgatgt cgtcgtcggt gacgacgtag
     4921 cgcgcggggc gcagtccgtt gcggtcgagt gtggcgccga tgcgcacgcc gtcggtgaac
     4981 gcaattgcgg ccgggccgtc ccacggctcc atcatcgccg cgtggtactc gtagaaagcg
     5041 cggcggttct cgtccatcaa cgtgtgctgc tcccacgcct ccggaatcat catcatcacc
     5101 gcgtgcgcga gcggataacc cgccatcgtc agcagctcga ggcagttgtc gaagcaggcc
     5161 gtgtcggact gcccggcgta gatcagcggc cacagcttct tcaggtcgtc cttcagcaca
     5221 ggcgaggcca tgacgccctc ccgcgcacga atccagttga agttgccctt gacggtgttg
     5281 atctcgccgt tgtgcgcgat catccggtac gggtgcgcaa gctcccacga cggaaaggtg
     5341 ttggtggaaa agcgctggtg caccagcgcg atcgccgaaa ccacttccgg ctcgcgcaga
     5401 tcgcggtagt aacggccgac ctgatcagcg agcagcaggc ccttgtagac gaccgtgcgc
     5461 gcggacatcg acggcacgaa gtactccttg ccgtgctgca acttcagcgc ctggatcttg
     5521 tggctcgcgg tcttccggat cacgtagagc ttgcgctcga gcgccgccgg caccatcaca
     5581 tcgggaccgc ggccaatgaa aatctgccgg atcatcggct ccttgtcgcg cacgaacgga
     5641 gacatcggca tgtcgcgatc gaccggcacg tcgcgccatc ccagcacgac ctgaccttcg
     5701 gcgcgtaccg tgcgttcgag ttcctgctcg cacgcgagcc gcgacgcgtt ctcgcgcggc
     5761 aggaagatca ttccgacgcc gtattcgccc ggcggcggaa gcgcgacgcc cagtttcacc
     5821 atctcgcgcc gatagaacgc gtcggggatc tggatcagta ttccggcgcc atcgccgcac
     5881 agcgggtccg caccgaccgc gccgcgatgg tcaaggtttt cgaggatcag cagcgcttgc
     5941 tggatcagcg agtggctcgc cttgcccttg atgttcgcca cgaa
//
LOCUS       DBNHBY010000103         1889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1223739, whole genome shotgun sequence.
ACCESSION   DBNHBY010000103 DBNHBY010000000
VERSION     DBNHBY010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1223739"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..476
                     /locus_tag="AC16HB_02515"
     CDS             51..476
                     /locus_tag="AC16HB_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5605692.1"
                     /translation="MANYTSSCACGAVQVELSGEPAVQVYCHCNSCRSWLSAPVHAAS
                     LWPTPNVRVVKGAENLGMYKRTDNSHRQFCKSCGAGVLVGHPSMGMTDIPAGTVAGMT
                     YRPTLHVNYGEKVMAVRDGLPKFKDFPKEFGGSGEMLPE"
     gene            complement(483..1220)
                     /locus_tag="AC16HB_02520"
     CDS             complement(483..1220)
                     /locus_tag="AC16HB_02520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5605693.1"
                     /translation="MQWVRSIVYTVLMIAVTAFFGVVVLVSALIPMPIERRYAIPRTW
                     GLILTGLAGTICGLRYTIEGRENLPSRPFVSLWKHSSAWDTMAQMFVVPPASWSLKRE
                     VLWIPIVGWAVAVFKPIAINRKAGQSAVNQVVRQGRERLASGMGVIVYPEGTRMPPGQ
                     TRKYGISGALLACETGAPVVPIAHTSGYFWRRRSLLKKRGTIRVVIGPPIDPTGLNPR
                     EVNERAQQWIEATIAATVAADETAHQP"
     gene            complement(1228..>1889)
                     /locus_tag="AC16HB_02525"
     CDS             complement(1228..>1889)
                     /locus_tag="AC16HB_02525"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR5605694.1"
                     /translation="TSIERRLDLDLQQLITQAVAAARADMATVAASQGKSLPPADAAA
                     LQNEVYDYVIERLRAYYVEGGAGIAVTPEMFDAVLATRPASPLDFDARLRALVEFLAL
                     PDAASLAAANKRIANILRKSTEPVGEQFDAARLIDPAEQVLAEQVVAIAREVEPRYAA
                     RDYTAALRQLAALRKAVDDFFDSVMVMADDPALRANRLALLNRMRGLFMRAADLSRLP
                     G"
BASE COUNT          307 a          648 c          649 g          285 t
ORIGIN      
        1 cgcggggata gactgcgcgc ttcaacgaca cacgcacagg ggaaaccatc gtggccaatt
       61 acacttcgag ctgtgcctgc ggcgccgtcc aggtcgagct cagcggcgag cccgccgtgc
      121 aggtctattg ccactgcaat tcgtgtcgca gctggctgag cgcgccggtg cacgccgcgt
      181 cgctgtggcc gacgccgaac gtgcgcgtcg tcaagggcgc agagaacctc ggcatgtaca
      241 agcgcaccga caacagccat cggcagttct gcaagagctg cggcgccggc gtactcgtgg
      301 ggcatccgtc gatgggcatg accgacatac cggccggcac cgtcgcgggc atgacctacc
      361 gcccgacgct gcacgtgaac tacggcgaaa aagtcatggc ggtgcgcgac ggcctgccga
      421 agttcaagga cttcccgaag gagttcggtg gctcgggcga aatgctgccc gagtaacgag
      481 cctcagggct ggtgcgccgt ctcgtccgcg gcgaccgtcg cagcgatggt cgcctcgatc
      541 cactgttgcg cgcgctcgtt gacctcgcgc ggattcagcc cggtcggatc gatcggcggg
      601 ccgatgacga cgcggatcgt gccccgtttc ttgagcaggc tgcgccgccg ccagaagtag
      661 cccgaggtgt gcgcgatcgg caccacgggc gcgccggtct cgcaggcgag cagtgcgccg
      721 ctgatcccgt acttgcgcgt ctggcccggc ggcatgcgcg tgccttcggg gtacacgatg
      781 acgcccatgc cggaggcgag acgctctcgc ccctgccgca cgacctggtt cactgcggac
      841 tgtcccgcct tgcggttgat cgcgatcggc ttgaacaccg cgacggccca gccgacgatc
      901 gggatccaca gcacttcgcg cttcagcgac cacgacgccg gcgggacgac gaacatctgc
      961 gccatcgtgt cccacgccga ggagtgcttc cacagcgaca cgaaaggccg cgacggcagg
     1021 ttctcccggc cctcgatcgt gtagcgcagg ccgcagatcg ttccggccag cccggtcagg
     1081 atgagtcccc acgtgcgcgg gatcgcgtaa cgccgctcga tcggcatcgg gatcagcgcc
     1141 gaaaccagca ccaccacgcc gaagaaagcg gtgaccgcga tcatcagtac ggtgtagacg
     1201 attgagcgca cccactgcat ggtcggctca gcccggcagt cgcgacaggt cggccgcgcg
     1261 catgaacaaa ccgcgcatgc gattgagcag cgcgagacgg ttggcgcgca gcgccgggtc
     1321 gtcggccatc accatgacgg aatcgaagaa gtcgtcgacg gccttccgca gagccgccag
     1381 ctgccgcagg gccgccgtgt agtcgcgcgc ggcgtagcgc ggctcgactt cgcgggcgat
     1441 cgcgacgacc tgttccgcca gcacctgttc cgccgggtcg atcaggcgtg cagcgtcgaa
     1501 ctgctcgccg accggctcgg tcgacttgcg caggatgttg gcgatgcgct tgttcgccgc
     1561 ggcgaggctc gcggcatccg gcagcgcgag gaactcgacc agcgcgcgca gccgggcgtc
     1621 gaagtccagc ggcgaggcgg gtcgcgtcgc gagcaccgcg tcgaacatct ccggcgtcac
     1681 ggcgatgcct gcgcctcctt cgacgtagta cgcgcgcagg cgctcgatga cgtagtcgta
     1741 aacctcgttc tgcaacgcgg ctgcatcggc cggcggcagg ctcttgccct ggctcgccgc
     1801 cacggtggcc atgtcggcgc gggcggcggc aacggcctgc gtgatcagct gctgcaggtc
     1861 gaggtcgagc cggcgctcga tcgacgtgc
//
LOCUS       DBNHBY010000104         2003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1697601, whole genome shotgun sequence.
ACCESSION   DBNHBY010000104 DBNHBY010000000
VERSION     DBNHBY010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1697601"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="AC16HB_02530"
     CDS             complement(<1..149)
                     /locus_tag="AC16HB_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5605695.1"
                     /translation="MTVRITGISSMATRQVLAELAAAYQRRSGVVVALESAGGVDASR
                     RVQAG"
     gene            374..1294
                     /locus_tag="AC16HB_02535"
     CDS             374..1294
                     /locus_tag="AC16HB_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5605696.1"
                     /translation="MDLKQLEYFVQVADLGSFTRASQVLRIAQPAMSRQVRTLELELR
                     QTLFERTGRGVTLTEAGKLLLAHGRGILQQVARARQDLEDHRGKPVGRLALGLPPSVS
                     RTLTGPLVEKFRQRFPKATLTIIEGLSIYALEWLVQGRIDCAVVYPVAPSAAVELEFV
                     LDEPLYLVTARGRRRGMGLIGPAVSLSDVAARELVIPSRPHSIRMAVEMALAGAGLEP
                     HIGLEIESVPAILDLVERTPLAAVLAVNALRGTAHERALVAQPIRNGNEVLATQLWIA
                     TSKQRPRGPLLEQGTALVRELLLQLWRHEP"
     gene            complement(1339..1926)
                     /locus_tag="AC16HB_02540"
     CDS             complement(1339..1926)
                     /locus_tag="AC16HB_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5605697.1"
                     /translation="MKSIRIIQDEHRSITAVIEGLRQLLADIEAGRTAPDFDLLDAMF
                     RYIEAFPERLHHPKEDEFLFARLRLRRPDAAPLLDRLHHEHEQGRIHFADLKAMFERY
                     RRDPRALRDFVERVERYAHFHWLHMRREEDEVLPLATAALSPEDWALIDAAFDSNSDP
                     VVGISATKEFRAMFRRIVSLAPPPLGVGPEVPARK"
BASE COUNT          327 a          680 c          677 g          319 t
ORIGIN      
        1 tcaccagcct gaacccgtct cgacgcgtcg acgccaccgg ccgactcgag cgcgaccacg
       61 acacccgagc gccgttgata cgccgcggca agttcagcga gcacctgacg cgtcgccatc
      121 gacgagatgc ccgtgatccg gacggtcatg cccggttccc gggtgacagc gcggcgcgcg
      181 gcacacgatc gcggcgcgca acgtcggccg acggatcgtc ggcgccaatg cgcgggtcgc
      241 gcggtgttcg cttgcgcgtc gacccgctgg gaatggcggc cttggacatg acgtgattct
      301 tgggcgtcac ggcctatcca gcaacgcgat cgctagacta cctgctagac cgattcgata
      361 tgacggcccg gacatggacc tgaagcagct ggagtacttc gttcaggtcg ccgacctcgg
      421 gagtttcacc cgggcctcac aggtgctgcg gatcgcccag cccgcgatga gccgacaggt
      481 tcgcacgctg gagctcgaat tgcgccagac gctgttcgag cgcaccggtc gcggcgtcac
      541 gctgacggaa gcgggcaagc tgctgctcgc gcatggccgc ggcatcctgc agcaggtcgc
      601 gcgggcgcgg caggatctcg aggaccatcg cggcaagccg gtcgggcgcc tcgcactcgg
      661 gttgcctcca agcgtcagcc gcacgctgac cgggccgctc gtcgagaaat ttcgccagcg
      721 gtttccaaag gcgacgctga ccatcatcga gggcctgtcg atctacgccc tcgagtggct
      781 cgtccagggg cggatcgatt gcgccgtggt gtatccggtt gcgcccagcg cggccgtcga
      841 actcgagttc gtactcgacg agccgctcta cctcgtgacg gcgcgtggcc gccggcgcgg
      901 gatgggcctg atcgggcccg ccgtctcgct gtccgacgtc gccgcgcgcg aactggtgat
      961 tcccagccga ccgcactcga tccgcatggc cgtcgagatg gcgctcgccg gcgccggact
     1021 ggagccgcac atcgggctgg agatcgaaag cgtgccggcg atcctcgatc tcgtcgaacg
     1081 aacgccgctc gcggccgtgc tggcggtcaa cgcgctgcgc gggaccgcgc acgagcgtgc
     1141 cctcgtcgcg cagccgatcc gcaacggcaa cgaggtgctg gcgacgcaac tgtggatcgc
     1201 gacgtccaag caacggccgc ggggcccgct gctcgaacaa ggaaccgcgc tggtacgcga
     1261 gttgctgctg cagttgtggc ggcacgagcc ttgagcgacc gctgaccggc aggacgtcgc
     1321 ttgggctcgt gtccccattc attttctcgc cgggacttcg ggcccgacgc ccaatggcgg
     1381 cggcgccagc gagacaatgc gacgaaacat cgcccggaat tccttcgtcg cactgatgcc
     1441 gaccaccggg tcgctgttcg agtcgaaggc ggcgtcaatc aacgcccagt cttccggcga
     1501 cagcgcggcg gtcgcgagcg gaagaacctc gtcctcctcg cgccgcatgt gcagccagtg
     1561 gaagtgggca tagcgctcga cgcgctcgac gaagtcgcgc agcgcgcgcg ggtcacgacg
     1621 ataacgctcg aacattgcct tcagatcagc gaagtggatg cgcccctgtt cgtgttcatg
     1681 gtgaaggcga tcgagcagcg gcgccgcgtc tggccggcgc aggcgcagac gcgcgaacag
     1741 gaactcgtcc tccttcggat ggtgcagccg ctccggaaac gcctcgatgt agcggaacat
     1801 cgcatcgagc agatcgaagt ccggcgccgt gcggccggcc tcgatatccg cgagaagttg
     1861 gcgcaggccc tcgatcaccg cagtgatcga gcggtgttcg tcctgaatga tccgaatcga
     1921 cttcatcgac gtgtgtcctt cccggccccg gtcaggccac ggtcaggagg cgctgcagtg
     1981 tctgctcatc ggcgagcagt gcc
//
LOCUS       DBNHBY010000105         2287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1562112, whole genome shotgun sequence.
ACCESSION   DBNHBY010000105 DBNHBY010000000
VERSION     DBNHBY010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1562112"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16HB_02545"
     CDS             complement(<1..225)
                     /locus_tag="AC16HB_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5605698.1"
                     /translation="MNTNQIARIAGMVGEPARTAMLIELMDGRALTAAELARAARITP
                     QTGSSHLAQLVDADLLRVTTSGRYRYYRLAS"
     gene            348..1628
                     /locus_tag="AC16HB_02550"
     CDS             348..1628
                     /locus_tag="AC16HB_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605699.1"
                     /translation="MAAISAQARSSFFGPAVVRGAFILALFGWGVGFYGPSVYLHAVE
                     QRTGWALTVVSSALTVHFVFGALVVTQLPKWHRRFGVGPTAVIGAALTSLGVLGWALA
                     AQPWQLFAAALASGGGWVTMGAVAVNAVIAPWYVRLRPAALAKAYNGASIGGVIFSPL
                     WVWLIATLGFASAAIAVGVVLFAVIALLARTVFARTPDVLGQRPDGDAPGASARSATP
                     ARARPLPGRRLWRDRRFVTLALGMAMGLFAQIGLVAHLFSLLVPALGAQRAGLTMGFA
                     TACAIAGRLIVARIMPLGADRRLVAAAGYLVQLTGSFVLLAASEAQVGLILLGVALFG
                     SGIGNATSLPPLIAQAEFVPEDVPRVVALIVALAQATYAFAPAVFGGVLTLSSGSAVG
                     IGHGVAGFFVSAALVQCIALACFLAGRAGAAARA"
     gene            1755..>2287
                     /locus_tag="AC16HB_02555"
     CDS             1755..>2287
                     /locus_tag="AC16HB_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605700.1"
                     /translation="MARKRAKVVRNVRPDAVDLRDLPFRPNVALTPAPQLFPPKDLQS
                     PVKYQGETSACTGFSLSLVVEHLLRAAQRDAPLISPYMLYSMARRYDEFPGSKDEGSS
                     LRGALKGWHKHGACADRLWTTGIDMPPAPTNAEDDWWLDAVRRPLGAYYRIDPRAISD
                     MHAALNEVGIVYASAVC"
BASE COUNT          271 a          803 c          824 g          389 t
ORIGIN      
        1 cgaagcgagc cggtaatagc ggtagcggcc cgaggtcgtc acgcgcagca gatccgcgtc
       61 gacgagttgc gccagatggc tgctccccgt ctgcggggtg atgcgcgcgg cgcgcgcgag
      121 ttcggcggcc gtcagcgcgc gcccgtccat cagttcgatg agcatggccg tgcgcgccgg
      181 ctcgccgacc atgccggcga tgcgcgcgat ctggttggtg ttcacgtcgc actcctgggt
      241 cgcggcccca gtatgcatgc gcgtgcgccg cccacatttc gaccgcggac gaagcgtcgg
      301 ccgccgcgtc cgccgcacga tggcagccgc aactcgtggc ggttcacatg gcggccatct
      361 ccgcgcaagc gcgctcttcc ttcttcggtc ccgcggtcgt gcgcggcgcg ttcattctcg
      421 cgctgttcgg ctggggcgtc ggcttctacg ggccgtccgt gtatctgcac gcggtcgagc
      481 agcgcacggg atgggcgctc accgtggtgt cgtccgcgct cacggtgcac ttcgtattcg
      541 gggcgctggt cgtcacccaa ctaccgaagt ggcatcgccg tttcggcgtc gggccgacgg
      601 cggtgattgg cgcggcgctg acgtcgctcg gcgtgctcgg ttgggccctt gccgcgcagc
      661 cgtggcagct ctttgccgcg gcgctcgcga gcggtggcgg ctgggtcacg atgggcgccg
      721 tggccgtgaa tgcggtgatc gcgccgtggt acgtgcggct gcgcccggcg gcgttggcca
      781 aggcctacaa cggcgcgagc atcggcggcg tgatcttctc tccgctctgg gtgtggctga
      841 tcgcgacgct cggcttcgcg tcggcagcca ttgccgtcgg cgtcgtgctc ttcgcggtga
      901 ttgcgctgct ggcgcgcacc gtgttcgccc gcacgcccga cgtgctcggc cagcgccccg
      961 atggcgacgc gcccggcgcg agcgcgcgga gcgccacgcc agcgcgcgcg cgacccctgc
     1021 cgggccggcg gctctggcgc gatcgccgtt tcgtgaccct ggcgctcggc atggcgatgg
     1081 gcctgttcgc gcagatcgga ctggtcgcgc atctgttctc gctgctcgtt cccgcgctgg
     1141 gcgcgcaacg cgcgggcctg acgatgggct tcgcgaccgc ctgcgcgatt gccggtcgcc
     1201 tgatcgtcgc tcgcatcatg cccctgggcg cggatcggcg tctcgtcgcc gccgccggct
     1261 atctcgtgca actgacgggc tcgttcgtcc tgctggctgc gagcgaagcg caggtcgggc
     1321 tgatcctcct cggcgtggcc ctgttcggct cgggcatcgg caatgccacg tcgctgccgc
     1381 cgttgatcgc gcaggccgag ttcgtgcccg aggatgtgcc gcgcgtggtg gcgctgatcg
     1441 tcgcgctggc gcaggcgacc tatgcgttcg cgccggccgt gttcggcggg gtgctcacgc
     1501 tttcgagcgg cagcgcggtc ggcatcgggc acggcgtcgc gggcttcttc gtatcggcag
     1561 cgctcgtcca gtgcatcgcg ctcgcgtgct tcctggccgg gcgcgcaggc gccgcagcgc
     1621 gcgcgtgagc gacacaacct gcaagacgcg cactcgctca tgccgccgaa ttcccacgcg
     1681 atcggcgatt gacgcgcgcc gtgcggccgc cctaccgtca cgcgtcgccg acgacggcac
     1741 gggaggaaat cgagatggca aggaagcgcg caaaggtcgt tcgcaacgtt cgccccgatg
     1801 cggtcgactt gcgcgacctg ccgttccggc ccaacgtcgc gctcacgccg gcaccgcaac
     1861 tgttcccgcc gaaggacctg cagtcgccgg tgaagtacca gggcgaaacg agcgcgtgca
     1921 ccggcttctc gctgtcgctg gtggtcgaac acctgctgcg cgcggcccag cgcgacgcgc
     1981 cgctgatctc gccgtacatg ctgtactcga tggcgcgccg ctacgacgag tttcccggct
     2041 cgaaggatga aggctcgagc ctgcgcggcg cactgaaagg ctggcacaag cacggcgcgt
     2101 gcgccgatcg gctgtggacc acgggcatcg acatgccgcc ggcaccgacg aacgccgagg
     2161 acgactggtg gctcgacgca gtgcgccggc cgctcggcgc gtattaccgg atcgatccgc
     2221 gcgcgatcag cgacatgcac gccgcgctga acgaggtcgg catcgtctac gcgagcgcgg
     2281 tgtgtca
//
LOCUS       DBNHBY010000106         4772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1223899, whole genome shotgun sequence.
ACCESSION   DBNHBY010000106 DBNHBY010000000
VERSION     DBNHBY010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1223899"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16HB_02560"
     CDS             <1..307
                     /locus_tag="AC16HB_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5605701.1"
                     /translation="RAADVRRRSLAGELRAEPRAALHERLGPDSGAAAALSTRECEVL
                     DWVAAGKSNAQISAILGISRRTVQKHLKHLYEKLGVESRTAAAMRVAAPRCRSAASR"
     gene            374..1213
                     /locus_tag="AC16HB_02565"
     CDS             374..1213
                     /locus_tag="AC16HB_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5605702.1"
                     /translation="MTLTVSFAVLLAALLHASWNAMIKGSRDVLLDTAGIVLGAGIVA
                     LPFLFFIAPPPAAAWPYIGTSLTIHLAYYYLMVNAYRVGDLTLVYPLMRGVAPLITGV
                     AGIALLRELPGTLAWTGMLLISGGVFLLAFRALGHAPSRAAIGFALANAAVIAAYTLV
                     DGKGARVSGNVWGYITWLFVLDAIPFGLYMLARHRARFGAYLSANVGRGLIGGGLSAA
                     AYAIAVWAMTQAPVALVASLRETSVLFATLIGASMLKEHLSPRRWAGVLAVVVGVVAL
                     KLA"
     gene            1259..2797
                     /locus_tag="AC16HB_02570"
     CDS             1259..2797
                     /locus_tag="AC16HB_02570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR5605703.1"
                     /translation="MRRSFQHLSGLDATFLHLESPATPMHVGGLHLFELPHGYRGDYC
                     DDVKRHVAARMHLLPAFRRKLVPMPFDLANPVWMRDPDVDLDYHVRRIVLSKPGSIAQ
                     LEAYVGRLHSSLLDRSRPLWEIYVIEGLADRQAAVYSKLHHAAVDGQAAVELARVLFD
                     TAPEGREIESPVEDEIEDEPTVRELLSAGARNSLEQFGKFGRMLPALAKGGAKLVAGG
                     LLPQRFGGGGTGLPRLARGPRTPLNVAITNQRNFAGASTSLADAKRISKVLDVTINDI
                     VLAVCSGALRRWLAAHGGVPDKPLVAAVPFSLRASGDASSNNQVSMMLADLATHLDDP
                     VQRLAAIRASMLKGKAIAGSLREGIPTDFPSIGAPWLLSGLAALYGRSRLADSMAPIA
                     NVVISNVPGPQMPLYLAGARMTSNFPVSIVVHGVALNITVQSYCGAIEYGLTACKRAV
                     PDLAKFKDHIVAAAAEYSQLAQMHVAAPSRPRAPARKRDGESASAPRPRRTRVKARTA
                     EAAS"
     gene            2794..3651
                     /locus_tag="AC16HB_02575"
     CDS             2794..3651
                     /locus_tag="AC16HB_02575"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR5605704.1"
                     /translation="MKKSPRSRPAPRPARSAARLAQEAAAIKNAIEWEREALSAPSLW
                     MLALEWRAPIEYGALLAALPWLQRAPNGDGHPVLVFPGLVAGDLSTAALRGFLRGRGY
                     DVHGWQQGLNFGARPGVIEACLARVMRLADQSKRKVSLIGWSLGGIYAREIAKDLPDY
                     VRCVVTLGTPFNGHARANNAWRLYEFAAGHAVDDHPRAAHLHRAPPVPTTSVYSRTDG
                     VVAWQSCINDAYPNSENIEVVASHFGIGMNPAALYAIADRLAQREEGWQPFHRDGWRA
                     LVYPEPPGN"
     gene            3651..4592
                     /locus_tag="AC16HB_02580"
     CDS             3651..4592
                     /locus_tag="AC16HB_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5605705.1"
                     /translation="MASLTANQIRIEYESHGDPAQPAIVLIMGLGMQLVAWPDELVEG
                     LVARGFRVVRFDNRDAGLSTQFDDYPAGSLTTALMRSMFGWPVAAPYKLDDMANDTIG
                     VLDALGIARAHMVGASMGGMIAQLVAVRHAARVASLTSIMSSSGARELPQPRREAFFA
                     LIKRPARGATLDQLVEHYVNLFRVIGSPAYSTPLPQLQERLQRGLARAYRPLGTSRQM
                     LAILASGSRSHLLPQITAPTLVIHGDADPLVPVAHGEDTAAKIPGATLKVMRGMGHDL
                     PPALMPELIELIAAHCAAADGETKGRPQPPFPVTGAD"
     gene            complement(4603..4752)
                     /locus_tag="AC16HB_02585"
     CDS             complement(4603..4752)
                     /locus_tag="AC16HB_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR5605706.1"
                     /translation="MSLFTTITHGSGIKVSSYDFEAKVPEPGSLALVGIGLLGLGARL
                     RRRKA"
BASE COUNT          713 a         1623 c         1666 g          770 t
ORIGIN      
        1 tcgcgctgcc gatgttcgtc gacgatcgct ggctggtgag cttcgtgcag agccgcgcgc
       61 ggcgctgcac gagcggctcg gcccggatag cggtgcggcg gcggcgctca gcacgcgcga
      121 atgcgaagtg ctcgattggg tcgctgccgg caagagcaac gcgcagatca gcgcgatcct
      181 cggcatcagc cgacgcaccg tgcagaaaca cctgaagcac ctgtacgaaa agctcggcgt
      241 ggaaagccgc accgccgcgg cgatgcgcgt cgcggcgccg cgctgccgtt ccgccgcatc
      301 ccgctgacgc gaccgtgcgc gttatgctcg cgccgccctg cgcaccggcg tgggacctcc
      361 ttgcgcgcgc gccttgaccc tcaccgtctc cttcgccgtg ctgcttgccg cgctgctgca
      421 cgcaagctgg aacgcgatga tcaagggcag ccgcgacgtg ctgctcgaca ccgcgggcat
      481 cgtgctcggc gcggggatcg tggcgctgcc gttcctgttc ttcattgcgc cgccgcctgc
      541 ggcggcctgg ccgtacatcg gcacatcgct gacgatccac ctcgcctact attacctgat
      601 ggtcaacgcg taccgcgtcg gcgacctgac gttggtgtat ccgttgatgc gcggcgtggc
      661 gccgttgatc acgggcgtgg ccgggatcgc gctgctgcgc gaactgcctg gcacgctggc
      721 gtggaccggc atgctgctga tctcgggcgg cgtgttcctg ctcgcgttcc gtgcgctcgg
      781 gcatgcccca tcgcgggcgg caatcgggtt tgcgcttgca aatgcggcgg tgatcgccgc
      841 ctacacgctg gtcgacggca agggcgcgcg cgtgtcgggc aacgtctggg gttacatcac
      901 gtggctgttc gtgctcgacg ccattccgtt cgggctgtac atgctggcgc ggcatcgcgc
      961 gcgattcggc gcgtacttga gcgcgaacgt ggggcgcggg ctgatcggcg gcgggttgtc
     1021 ggccgccgcc tatgcgatcg cggtctgggc gatgacgcag gcgccggtgg cgctggtggc
     1081 gtcgctgcgc gagacctcgg tgctgttcgc gaccttgatc ggcgccagca tgctgaaaga
     1141 acatctgtcg ccgcgccgct gggccggggt attggcggtg gtggtcggcg tcgtcgctct
     1201 gaagttggcc tgatctggta agttccgccg cacaatggac atggcgcgca gggagacgat
     1261 gcgacgcagc ttccagcacc tgagcgggct cgacgccact ttcctgcatc tggaatcgcc
     1321 cgcgacgccg atgcacgtcg gcggcctgca cctgttcgaa ctgccacacg gctaccgcgg
     1381 tgactactgc gacgatgtga agcgccacgt cgctgcgcgc atgcacttgc tgccggcgtt
     1441 ccgccgcaag ctcgtgccga tgccgttcga tctggccaat ccggtgtgga tgcgcgatcc
     1501 cgacgtcgac ctcgactacc acgtgcgccg catcgtgttg agcaagccgg gctcgatcgc
     1561 ccaactcgag gcgtacgtcg gccgcctgca ttcgagcctg ctcgaccgta gccgcccgct
     1621 gtgggagatc tatgtgatcg aggggctggc cgacaggcag gcggcggtct actccaagct
     1681 gcaccatgcg gcagtcgacg gccaggcggc ggtcgaactg gcgcgcgtgc tgttcgacac
     1741 cgcgccggag ggccgcgaga tcgaatctcc ggtcgaggac gagatcgagg acgagccgac
     1801 cgtgcgcgaa ctgctgtccg caggcgcgcg caattccctg gagcagttcg gcaagttcgg
     1861 aagaatgctg ccggcgctcg ccaagggcgg cgcgaagctc gtcgccggcg gcttgctgcc
     1921 gcaacgattc ggcggcggcg gcaccgggct gccgcgactc gcgcgcggtc cacgcacgcc
     1981 gctgaatgtc gcgatcacca accagcgcaa tttcgccggc gcctcgacct cgctcgccga
     2041 tgcgaagcga atctcgaagg tgctcgatgt cacgatcaac gacatcgtgc tggcggtttg
     2101 cagcggcgcg ctgcggcgct ggctcgccgc gcatggcggc gtgccggata agccgctggt
     2161 ggcggccgtg ccattctcgc tgcgcgccag cggcgacgcg tcgagcaaca accaggtgtc
     2221 gatgatgctg gcggatctcg ccacgcacct ggacgacccg gtgcagcggc tcgcggcaat
     2281 ccgggcgtcg atgctgaagg gcaaggcgat cgccggctcg ctgcgcgaag gcattccgac
     2341 cgactttcct tcgatcggcg cgccgtggtt gttgagcggg ctggcggcct tgtacgggcg
     2401 cagtcggctt gccgactcga tggcgccgat cgccaacgtg gtgatctcga acgtgcccgg
     2461 cccgcagatg ccgctgtatc tggccggcgc acggatgacg agcaactttc cggtgtcgat
     2521 cgtcgtgcac ggcgtcgcgc tgaacatcac cgtgcagagc tactgcggcg cgatcgagta
     2581 cggcttgacc gcctgcaagc gcgcggttcc cgatctggcg aaattcaagg atcacatcgt
     2641 cgctgccgcc gccgaatact cgcagctcgc gcagatgcac gtggccgcgc cgtcgagacc
     2701 gcgggcgccg gcgcgcaaac gtgacggcga atccgcgtct gcaccgcgcc cgcgtcggac
     2761 acgcgtcaag gcacgcactg cggaggcggc gagttgaaga agtccccgcg ctcgcgcccc
     2821 gcgccgcggc ccgcgcgcag cgccgcgcgt ctggcgcagg aagcggcagc gatcaagaat
     2881 gcgatcgagt gggagcgcga ggcgctgagc gcgccatcgc tgtggatgct ggcgctcgaa
     2941 tggcgcgcgc cgatcgaata cggcgcattg ctcgccgcgc tgccctggct gcagcgcgcg
     3001 ccgaacggcg acggccaccc ggtgctggtc tttcccggac tcgttgccgg cgacctgtcg
     3061 accgccgcgc tgcgcggatt cctgcgcgga cgcggctacg acgtgcacgg ctggcagcag
     3121 ggactgaact tcggcgcgcg ccccggcgtg atcgaggcgt gcctcgcgcg cgtgatgcga
     3181 ctggccgacc agtcgaagcg caaggtgagc ctgatcggct ggagcctggg cggcatctac
     3241 gcgcgcgaga tcgcgaagga tctgccggac tacgtgcgct gcgtggttac gctcggcaca
     3301 cccttcaacg gccacgcccg cgcgaacaat gcctggcggc tgtacgagtt cgcggcgggg
     3361 catgcggtcg atgatcaccc gcgtgctgcg cacctgcacc gcgcgccgcc ggtgccgacg
     3421 acctcggtct acagccgcac ggacggcgtg gtcgcgtggc agagctgcat caacgacgcc
     3481 tatccgaact ccgagaacat cgaggtcgtc gccagccact tcggcatcgg catgaatccg
     3541 gctgcgctat acgccatcgc cgatcggctg gcgcagcgcg aggagggctg gcagccgttt
     3601 caccgcgacg gctggcgcgc gctggtgtat cccgaaccgc cggggaactg atggcaagcc
     3661 tcaccgccaa ccaaatccgg atcgagtacg aatcgcacgg cgatcccgcg cagcccgcga
     3721 tcgtgctgat catgggtctc ggcatgcagc tcgtcgcctg gccggacgag ttggtcgaag
     3781 gcttggtcgc gcgcgggttt cgcgtcgtac gcttcgacaa ccgcgacgcg gggttgtcga
     3841 cgcagttcga cgattacccg gcgggttcgc tgacgaccgc gctgatgcgc agcatgttcg
     3901 gctggccggt ggccgcaccg tacaagctcg acgacatggc gaacgacacg atcggcgtgc
     3961 tcgacgccct cggcattgcg cgcgcccaca tggtcggcgc atcgatgggc gggatgatcg
     4021 cgcaactcgt cgcggtccga catgcggcgc gcgtggcatc gctgacctcg atcatgtcga
     4081 gcagcggcgc gcgcgagttg ccccagccca ggcgcgaagc gttcttcgcg ctgatcaagc
     4141 gacccgcgcg tggcgccacg ctcgaccagc tggtcgagca ctacgtgaac ttgttccgcg
     4201 tgatcggtag cccggcctac tcgacgccgc tgccgcaact gcaggagcgg ctgcaacgcg
     4261 gtctcgcgcg cgcgtatcga ccgctgggca cgtcgaggca gatgctcgcg atcctcgcgt
     4321 caggcagccg ctcgcacctg ctgccgcaga tcaccgcgcc gacgctggtg attcatggcg
     4381 acgccgaccc gctggtgccg gtcgcgcacg gcgaagacac cgcagcgaag atccccggcg
     4441 cgacgctgaa ggtcatgcgc ggaatgggac acgacctgcc gcccgcgctg atgccagagc
     4501 tgatcgagct gatcgccgcg cactgcgccg cagccgacgg cgaaacaaaa gggcggccgc
     4561 agccgccctt ccctgtcacc ggcgctgatt gacgcgcgcc gttcaggcct tgcgccggcg
     4621 caagcgggcg ccgagtccaa gcaggccgat gccgaccaac gcgagcgagc ctggctcggg
     4681 gaccttggct tcgaagtcgt aactcgagac ctttatgccg ctgccgtgcg tgatggtcgt
     4741 gaacagcgac atcgaataca ggcttgccag at
//
LOCUS       DBNHBY010000107        14849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_953541, whole genome shotgun sequence.
ACCESSION   DBNHBY010000107 DBNHBY010000000
VERSION     DBNHBY010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_953541"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16HB_02590"
     CDS             complement(<1..272)
                     /locus_tag="AC16HB_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605707.1"
                     /translation="MRQLKQLLATLDRAPRQLVVGVRQDRTDGRGARVVGADGSVTLT
                     KRRLTGNAHVEASDTRTSGTSDTAQTVRVLEGSRAHIAIGAAIPLT"
     gene            complement(423..1256)
                     /locus_tag="AC16HB_02595"
     CDS             complement(423..1256)
                     /locus_tag="AC16HB_02595"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5605708.1"
                     /translation="MKAIVSELNLYPIKGCRGLSVRSAQLPATGLEVDGIGDREWVVV
                     DADGRFLSQREHPRMALIEPRFVGGSMRLKAPGMLALEVPFASEGDVVEVSVWDDRVA
                     AVTQGEIADAWFSNFLGVVARLVRFDPEGRRLARRKYTGTVDAPYKFADAFPLLITGR
                     ASLGDVNSRLAKNGETAVTMARFRPNLVLDGLPEYEEDYIGWLRRDGLVLRAVKPCAR
                     CTVPGVDPLTGEASTIIPDLLATFRGSADGVMFGVNAIVVEGAGVTLAVGDEFTVELN
                     V"
     gene            1448..2323
                     /locus_tag="AC16HB_02600"
     CDS             1448..2323
                     /locus_tag="AC16HB_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605709.1"
                     /translation="MDEDTRGRDRFLDGIASAHRSADRQRLSAALVDAARAIRAKRDD
                     GAAADGALLTRCEALLQSARAAAESATHLVAWNCLHQIERELAAAMDESQRRAALVAY
                     RAEAGALGHLPRAAIEALAGEGGGSTADLQALMRIAHAAREERLYRAEQARRQVAALT
                     TLLMIAVVFLSGWALAGGFEWILHEDVEVTLAMALVNGMLFGFLGGLLSLVFGLLRGD
                     GRGGVWAATIARPFVGAVVAIPIAFFLQSGLLNLGNVTPALDLALCFLGGFSERWFVR
                     QFSRPDTGRHAGRAD"
     gene            complement(2350..3444)
                     /gene="ychF"
                     /locus_tag="AC16HB_02605"
     CDS             complement(2350..3444)
                     /gene="ychF"
                     /locus_tag="AC16HB_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR5605710.1"
                     /translation="MALRCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     AVPDPRLDALAKIARPEKVIPATVEFVDIAGLVAGASKGEGLGNQFLANIRECDAIAH
                     IVRCFEDPNVIHVAGKVDPVSDIEVINTELALADLATVEKQLARYSKVAKSGGDKEAK
                     RLVDALEKILPALNDAKPVRSVDLYAEERAVLRPLFLLTMKPTMYVANVEEHGFENNP
                     LLDKVKARAAPEGSPVVAVCAKVEAEIADLSDADKQVFLADMGMTEPGLARVIRAGYE
                     LLGLQTYFTAGPKEVRAWTVSRGATAPQAAGVIHTDFEKGFIRAETIAFDDYVKYNGE
                     HGAKEVGKMRLEGKDYVVQDGDVMHFRFNV"
     gene            complement(3533..4591)
                     /locus_tag="AC16HB_02610"
     CDS             complement(3533..4591)
                     /locus_tag="AC16HB_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5605711.1"
                     /translation="MSAERSFVVHAGARAAAHVRANGVAPADIACIPAAAGGPKGLTL
                     IPLDKLLFGAWLRDVKHIELIGASIGAWRMAAASMPDPLAALERLSEGYLAQTYPRNP
                     TTTYVSDECRKLARAVLGGARAIEPRAGVGLSIIATRARGVLQANRSKAAFAKAALAN
                     TLSRARLAAHMERVIFHSTAATFPARAPDAFDLTRIPLDEHNAEDALLASGSIPLVCD
                     PVSDIAGAPRGNYWDGGLIDYHLLLPYRELDGLVLYPHFVPYVTPGWLDKFLPWRQHP
                     RAHPWLDNVLLISPSPALQARLPNRKLPDRNDFYRYGADHAARVRDWRHAIAESARFA
                     DETMAWLGRPDPSVMQAL"
     gene            complement(4588..5202)
                     /gene="pth"
                     /locus_tag="AC16HB_02615"
     CDS             complement(4588..5202)
                     /gene="pth"
                     /locus_tag="AC16HB_02615"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5605712.1"
                     /translation="MSDNDNSIRLVVGLGNVGAEYERTRHNAGFWFVDAVARKAGAHL
                     AHERKFHGEVARLKDGARELWLLKPGTYMNRSGQAVVALALYYKILPTQILVVHDEVD
                     LLPGVVKLKRGGGTAGHNGLKDIQARLTTPDFWRLRIGVGHPRALALSQEVADFVLHP
                     PGRDHQVAIEDAITRALDVVPALIDGQFERAMMKLHSVPKTPSA"
     gene            complement(5264..5959)
                     /locus_tag="AC16HB_02620"
     CDS             complement(5264..5959)
                     /locus_tag="AC16HB_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR5605713.1"
                     /translation="MKVVATTRAKQGTSASRSLRRANKVPGIIYGGNGSPVNIEIDHN
                     PLFHSLRKEKFHASILDMELDGRGERVLLRDFQMHPYKPQVLHIDFQRVSEDQKIHMR
                     VPLHFVGQENSPAVKEAGGLISHVLSDVDVACLPKDLPEYIEVDLSGLRAHETIRVRD
                     LKLPAGVTAVLKGKENPVVVSVSTKGEEEVVEAEAAVVPAAEVPATAQKAPPAAAAPA
                     APEKKGGDKEKKK"
     gene            complement(6010..6969)
                     /locus_tag="AC16HB_02625"
     CDS             complement(6010..6969)
                     /locus_tag="AC16HB_02625"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR5605714.1"
                     /translation="MVPMVPENLVVFTGNANPRLAHAVVQHLNIPLGRATVSRFSDGE
                     VMVEINENVRGKDVFVLQSTCQPTNDNLMELVIMVDALKRGSAGRITAAIPYFGYARQ
                     DRRPRSARVAISAKVVANMLQAVGVHRVLTMDLHADQIQGFFDIPVDNIYASPILLGD
                     LWKHNIDNLMVVSPDVGGVVRARALAKRMDADLAIIDKRRPRANVAEVMHIIGDVAGR
                     TCVIMDDMIDTANTLCHAAAALRDHGAAKVMAYCTHPVLSGKAVERIMNSVLDEVVVT
                     DTIPLREDAQKCPKIRQLSCAALLGETISRISREDSVSNLFIE"
     gene            complement(7024..7100)
                     /locus_tag="AC16HB_02630"
     tRNA            complement(7024..7100)
                     /locus_tag="AC16HB_02630"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7064..7066),aa:Gln,seq:ttg)
     gene            complement(7136..7993)
                     /gene="ispE"
                     /locus_tag="AC16HB_02635"
     CDS             complement(7136..7993)
                     /gene="ispE"
                     /locus_tag="AC16HB_02635"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR5605715.1"
                     /translation="MTRLFLGVPAPAKLNLCLHVLGRRADGYHELQSVFVPIDLADTL
                     DFELREDGAITRGGDVIGDPQRDLALRSARLLQQQSGTKLGVAIRVDKRIPAGSGMGG
                     GSSDAASTLIALNRLWRLGWPRADLAALAPRLGADVAFFLGTGPAVVEGIGERVTPIE
                     VPSAWFAVVKPQAAVATSEIFGDPGLTRDTKATIISAFSAGLAQFPRALFGANDLEVV
                     VRRRIPEVDAALTLLARFGTARMTGSGSAVFVATASEERARGAIAGLPDGWTGWSVRG
                     LKQHPLAEW"
     gene            complement(8001..8561)
                     /locus_tag="AC16HB_02640"
     CDS             complement(8001..8561)
                     /locus_tag="AC16HB_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015512.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HIR5605716.1"
                     /translation="MRAAAALAVAGVLLGACTTLAPVREAERVHQGRFALTATWPDRS
                     ESSSGRFSLAIHADGVTLDLSSPFGNTLARIDTDRSGARLTAPAAEGELKRLQGPNAD
                     ALAEQVLGWSLPVSGIGDWIVGRPTPSRPYRTLDSGSIEQDGWVIHVLDRFGVDGAPR
                     RLSFERPATMGSPAVIVRLVLDDPSS"
     gene            complement(8568..10358)
                     /locus_tag="AC16HB_02645"
     CDS             complement(8568..10358)
                     /locus_tag="AC16HB_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5605717.1"
                     /translation="MRIRLGLPALLVGTIVGCTSVAQPTAPVGPGKPAEGVPAPRLDA
                     LPDVDLTGALLYQIVAAEVALQRGDAGSAFATYMNVARTTHDPRLARRAAEIAFGARA
                     AEQALEAAQLWRELAPHSSEAAQTLAAIQIANGRYQDAAPLLAQQIASAASPVDELAR
                     VQRTLARGPDRASGFQLLESLAAPYLSDPAHGGDVRLIIASGAHAAGLVQRAAQEARA
                     SVELQPDSERASLAAAQFLARPDGKDSAEGRKQALELLRNFLQRRPEAADARLTYARL
                     LIADNDLPAARQQFADMLKRDANNPDALYAMGVLTLDGKPPRTESRAYFERYLKAVEA
                     APGTRDPDPAYMNLARIAEEERKYDEAIAWLERIDDGEQQLPARLRQALLLGKLKRVD
                     EARSLLADLAAQPSRSDEEKVQVVVAEGQLLREARRYRESFDLLAAALAKTPDNTNLL
                     YDTAMAAEKLDRIDLMETHLRRMMQLKPDDAHAYNALGYTFADRNLRLAEARDLIAQA
                     LKLSPNDGYILDSMGWVFYRLGDLPKAREYLERAWKGRPEGEVGAHFGEVLWLSGDRD
                     AARRVWREALAAEPDNELLRATLARFKVKP"
     gene            10409..11257
                     /gene="mutM"
                     /locus_tag="AC16HB_02650"
     CDS             10409..11257
                     /gene="mutM"
                     /locus_tag="AC16HB_02650"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR5605718.1"
                     /translation="MPELPEVEVTRRALAPYVEGEQVQAVIVRDRRLRWPVPARLAVA
                     LQGQRIERLERRGKYLLWRFAHGTLISHLGMSGSWRVHPGTVPPAGAHDHVDIIVAGR
                     PRSTLRLTDPRRFGALLWHPVRRGGAERHPLLSHLGAEPFDPRFDADHLFHHTRNRRS
                     SIKQVLLAGDVVVGVGNIYASESLFRAGIDPRTAAFRLTRARCERLVAAVRAVLTEAI
                     EVGGSTLRNFVGADGAEGYFMLEAFAYDRAGQPCRVCRTPIRRILQGQRATYFCPHCQ
                     TRRGRV"
     gene            11349..13367
                     /locus_tag="AC16HB_02655"
     CDS             11349..13367
                     /locus_tag="AC16HB_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR5605719.1"
                     /translation="MAQLASSFQEYGAWRDSLAHSVTQLRTWLQGNQLLDHDAARRLA
                     LGLDRLAEDKLVIAFVAEFSRGKSELINAIFFADYGRRILPSSAGRTTMCPTELMYDE
                     SLPPCIRALPIETRARQGTMSDFKKAGAEWRVFPVDVDAPEGMLQAFRLVADTVRVSV
                     QEALLYGLYDPSDPDQQTAVAADGTIEISRWRHAVINFPHPLLKRGLVILDTPGLNAI
                     GAEPELTLSLVPSAHAVLFVLAADTGVTRSDLDVWRQVVHDTGAATNHIAVLNKIDGL
                     WDGLKTAHEIDGEVDRQVQAVGHTLELDTARVFPVSAQKGLVAKVMRDERLLAASRLP
                     KLESALVDLLVPAKQRIVGSQTLAVVERIGEEVRQALSARERGVIEQLYELRALQGKN
                     QSSIERMSRRAQSESREFEANVRKLVATRVVLTRLSDQATTPLSHETLRTTLEATRER
                     MRKVRLTPQFYSSIKDYFAELQQHLRDANTRLAEIEQMVIGVQQQFASELGWSLPPPM
                     PFTLDKYRGDLEQVERAARSHFGAFVVMTRDKWSLIERFFDTVVARTREIFTTAERDT
                     EAWIRSLLPPIESQVREQRAQLKKRAESVERIRTAQESLDSRIGELEAALEDTHGKLE
                     ALRAGLARVRLVGAACPVTRTEAPDADAAAMPVRRTDPTEPSYLRVEI"
     gene            13367..14404
                     /gene="mutY"
                     /locus_tag="AC16HB_02660"
     CDS             13367..14404
                     /gene="mutY"
                     /locus_tag="AC16HB_02660"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR5605720.1"
                     /translation="MPRFAQRIVAWQREFGRRDLPWQHTRDPYRIWLSEIMLQQTQVA
                     TVAPYYLRFVERFPDVASLARADVQEVLQLWAGLGYYARARNLHACARRVVAEHGGRF
                     PCGAIALAQLPGIGRSTAAAIAAFCFGERAAILDGNVKRVLARHFGVEGFPGAAAVER
                     ELWARADAVLPEAGGMPAYTQGLMDLGATVCTRSDPLCGRCPVRATCIARQTNRVREL
                     PAPRPRQPVRTRSAHLLLLQHADCILLERRPYAGIWGGLLAPPQFDRIEDLSLALDHL
                     APGTTARALPSRRHAFTHFTLHFTPHLARFEHPVAVMEPRLHWLARADIKQAALPTPI
                     RTLLRDVLAAG"
     gene            complement(14421..>14849)
                     /locus_tag="AC16HB_02665"
     CDS             complement(14421..>14849)
                     /locus_tag="AC16HB_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5605721.1"
                     /translation="WDMQKLGVLNIRTLGTQRFRVRHAAAQPDGLLVGDVEEIAADVD
                     APLAPEHQPCATLLARIIDDLKSQREAALRDHPDDAGMLAANAIERPHMLHSSVWVGN
                     RLCEVLPVSLRAKQKLMELDDARTRLDIVQRFLRQHAVIR"
BASE COUNT         2302 a         5023 c         5013 g         2511 t
ORIGIN      
        1 aaggtgagcg ggatcgctgc gccgatcgca atgtgggcgc ggctgccctc gagcacgcgg
       61 accgtctgcg ccgtgtccga ggtgccgctg gtgcgtgtgt cgctcgcctc gacgtgcgcg
      121 ttgccggtca agcgccgctt ggtcagggtg accgatccat cggcgccgac gacgcgcgcg
      181 ccgcgcccgt ccgtacggtc ctgtcgcacg cctaccacga gctgccgcgg cgcgcgatcg
      241 agtgtggcca gcagctgctt cagctgtcgc acgttggcag ccgatgcgcg cacgaacagc
      301 tgaaagccgt gtccggtcag gcgccgcctg gttcgaggaa tgcgcgcaga ttgggcagca
      361 cctcgtccgt ggtccggtgc ctcatgtgga tgatctcgat ttcctgcgcg cgctggcgca
      421 ggtcagacat tcagctcgac tgtgaattca tcgccgaccg cgagcgtgac gcccgcaccc
      481 tcgaccacga tcgcgttgac gccgaacatc acgccgtcgg cgctgccgcg gaaggtcgcc
      541 agcaggtcgg ggatgatggt tgacgcctcc ccggtcagcg gatcgacgcc aggcacggtg
      601 caacgcgcgc atggcttgac tgcacggagg acgagaccgt cgcggcgcag ccaaccgatg
      661 tagtcctcct cgtattccgg caggccgtcg agcacgaggt tgggacggaa cctcgccatc
      721 gtcactgccg tctcgccatt cttcgccagc cgcgaattga cgtcgccgag cgatgcgcgg
      781 ccggtaatca gcagcggaaa cgcatcggcg aacttgtatg gcgcgtcgac agtacccgtg
      841 tacttgcgtc gggcaaggcg tcgcccttcg ggatcgaagc gcaccagccg cgcaaccacg
      901 ccgagaaagt tgctgaacca cgcatcggcg atctcgccct gcgtgacggc ggcgacgcga
      961 tcgtcccaca cgctgacctc gaccacgtcg ccttcggacg cgaaagggac ctcgagcgcc
     1021 agcatgccgg gagccttcaa ccgcatcgat cctccgacga agcgcggctc gatgagcgcc
     1081 atgcgcgggt gctcgcgctg gctcaggaac cggccatccg cgtcgacaac gacccattcg
     1141 cggtcgccga tgccgtcgac ttcgaggccg gtggcaggca gttgtgcgct gcgcacggaa
     1201 agaccgcggc agcccttaat tggatacagg ttcagttcgg agacgattgc tttcatgagg
     1261 cggagtttat cgaccgagcg gcgccttcag tgcatcgcgc gcccgcaccg gcgcatgcga
     1321 gcgcgatcga cgcgaatgca gcgaacaggg ttcggcgcgg catcaaaccg acgggcgtga
     1381 ttggtgccgg ctatgctacc gagcggaagc cactttgcgc attcgcgccg gcaacggaag
     1441 atcgaagatg gacgaggaca cgcgtgggcg tgaccgattt cttgacggca tcgcatccgc
     1501 gcatcgcagt gccgaccggc agcggctgag cgcggcgctg gtcgacgccg cgcgcgcgat
     1561 ccgcgcgaag cgcgatgatg gggcggcggc cgacggcgcg ctgttgacgc gctgcgaagc
     1621 gctgcttcag tctgcacgcg ctgcagccga gtccgcaaca catctcgtcg cttggaattg
     1681 cctgcaccag atcgaacgcg aactcgcagc ggcgatggac gaatctcaac gccgcgccgc
     1741 gctagtcgcg taccgggccg aggccggggc gctcggccac ttgccgcgcg cggcgatcga
     1801 ggcgcttgcc ggcgagggcg gcggcagcac agcggacctg caggcgctga tgcgcattgc
     1861 gcatgcagcg cgcgaagagc ggctgtatcg cgccgagcag gcgcgccgtc aggtcgcggc
     1921 gctgacaaca ttgctgatga tcgccgtcgt gttcctgagc ggctgggcgc tggcgggcgg
     1981 cttcgagtgg attctgcacg aggacgtcga ggtgacgctg gcgatggcgc tggtcaacgg
     2041 catgctgttc ggcttcctgg gcggcttgct gagcctcgtg ttcggcctgc tgcgcgggga
     2101 tgggcggggc ggggtctggg cggcgacgat cgcgcgtccg ttcgtgggag cggtggtcgc
     2161 gatcccgatt gcattctttc tgcaatccgg cctgctgaac ctcggcaacg tgacgccggc
     2221 gcttgacctc gcgttgtgct tcctcggtgg cttctccgaa cgctggttcg tgcgccagtt
     2281 cagccgcccc gacaccggac ggcacgccgg ccgcgccgat tgagtcagcg gcgaccgcgt
     2341 ccgccgcgtt cagacgttga accggaagtg catgacgtcg ccgtcctgca cgacgtagtc
     2401 cttgccttcc agtcgcatct tgccgacctc cttggcgcca tgctcaccgt tgtacttcac
     2461 gtagtcgtcg aacgcgatcg tctcggcacg aatgaagccc ttctcgaaat cggtgtggat
     2521 cacaccggcc gcctgcggtg ccgtcgcgcc gcgtgagacg gtccacgcac gtacttcctt
     2581 cggccccgct gtgaaatacg tctgcagtcc gagtagttcg tagccggcgc ggatcacgcg
     2641 ggcaagcccc ggctcggtca ttcccatgtc ggcaaggaac acctgcttgt cggcgtcgga
     2701 caggtccgcg atctcggcct cgactttcgc gcataccgcc accactggcg atccttccgg
     2761 cgccgcgcgc gccttcacct tgtcgagcag cggattgttc tcgaatccgt gctcctcgac
     2821 gttggcaacg tacatcgtcg gcttcatcgt cagcaggaac agcggtcgca gcacggcccg
     2881 ttcctctgca taaagatcga cgctgcgcac cggcttggcg tcgttcaacg ccggcaggat
     2941 cttctccagc gcatcgacca atcgcttcgc ctccttgtcg ccgccgctct tcgcgacctt
     3001 gctgtacctc gcgagctgct tctcgaccgt cgccaaatcg gccagggcga gctcggtgtt
     3061 gatcacctcg atgtccgaca ccggatcgac cttgcccgcg acgtggatca cgttcgggtc
     3121 ctcgaagcag cgcacgatgt gcgcgatcgc atcgcactcg cggatgttgg cgaggaactg
     3181 gttgccaagc ccttcgccct tgctcgcgcc ggcgaccagt ccggcgatgt cgacgaactc
     3241 gaccgtggcc ggaatcacct tttcgggcct ggcgatcttc gcgagcgcgt cgagccgtgg
     3301 gtccggcacc gcgacgattc cgacgttcgg ctcgatcgtg cagaacgggt agttctcggc
     3361 ggcaattcct gccttcgtga gcgcgttgaa gagcgtcgat ttgcctacgt tgggcaggcc
     3421 gacaatgccg catctgagag ccatgatgat ccttagagcg aagccgccgc gcgcggttcg
     3481 cgccgcgggc aacggacgaa ttatcgcgtg cggcgcgtgc gcgccgccgc ggtcacagcg
     3541 cctgcatcac cgaagggtcg ggtcgcccaa gccaagccat ggtctcgtcg gcgaagcgcg
     3601 cgctctcggc gatcgcgtgc cgccaatcgc gcacgcgggc cgcgtggtcg gcgccataac
     3661 ggtagaagtc attgcggtcg ggcagcttgc gattgggcag ccgcgcctgc agggcgggcg
     3721 agggcgagat cagcaggacg ttgtcgagcc agggatgggc gcgggggtgc tggcgccacg
     3781 gcaggaactt gtcgagccag ccgggcgtga cgtacggcac gaagtgtggg tacagtacca
     3841 gtccgtccag ttcgcgatac ggcagcagca ggtggtagtc gatcagccca ccgtcccagt
     3901 aattgccgcg cggggcgccg gcgatgtcgg acaccgggtc gcataccagc gggatcgacc
     3961 ccgacgccag cagcgcgtct tccgcgttgt gctcgtcgag cgggattctc gtcaggtcga
     4021 acgcatcggg cgcgcgagca gggaatgtcg ccgcggtgga atggaagatc acgcgctcca
     4081 tgtgcgcggc gagccgtgcg cgcgacagcg tgtttgccaa cgcggccttt gcgaacgcgg
     4141 ccttcgagcg gttcgcctgc agcacgccgc gcgcccgtgt ggcaatgatc gacagtccga
     4201 cgccggcgcg gggttcgatc gcacgcgcgc cgccgagcac tgcgcgcgcg agcttgcggc
     4261 attcgtcgct gacgtacgtg gtggtcggat tgcgtggata cgtctgcgcg aggtagccct
     4321 ccgacaatcg ttcgagcgcg gcgagcggat cgggcatcga cgcggccgcc atccgccacg
     4381 caccgatcga tgcgccgatc agctcgatgt gcttgacatc gcgcagccat gcaccgaaca
     4441 gcagcttgtc gagcggaatc agcgtcagtc ctttcggtcc gccggctgcc gctggaatgc
     4501 acgcgatgtc ggctggcgcg acgccgttgg cgcgcacgtg cgcagccgcg cgtgcgccgg
     4561 cgtgcacgac gaacgaccgc tccgcgctca tgcactcggt gtcttcggta ccgagtgcag
     4621 cttcatcatc gcgcgttcga attgcccgtc gatcagcgcc ggcacgacgt cgagcgcgcg
     4681 cgtgatcgca tcctcgatcg cgacctggtg atcgcggccg ggcggatgca gcacgaagtc
     4741 ggcgacctct tgcgaaaggg cgagcgcgcg cggatggccc acgccgatgc gcagccgcca
     4801 gaagtcgggc gtggtgagcc gtgcctgaat gtctttcagc ccgttgtggc cggcggtgcc
     4861 gccgccgcgc ttcagcttca cgacgcccgg cagcaggtcg acttcgtcgt gcacgaccag
     4921 gatctgcgtc ggcaggatct tgtagtacag cgcgagcgcc accacggcct gccccgagcg
     4981 gttcatgtag gtgccgggct tcagcagcca cagttcccgc gcgccgtcct tcagtcgcgc
     5041 gacttcgccg tggaacttgc gctcgtgcgc aagatgcgcg ccggccttgc gggcaactgc
     5101 gtcgacgaac cagaagcccg cgttatgacg cgtgcgctcg tactcggctc caacgttgcc
     5161 gaggccgacg accagccgga tcgaattgtc gttgtcgctc atggttcaaa caaaacggcc
     5221 cgcggtgtcg ccacgcgcgg gccgcattgt gcagtacgcg ggcttacttc ttcttctcct
     5281 tgtcgccgcc cttcttctcg ggcgcggcgg gcgcagcggc agcggggggc gctttctgcg
     5341 cggtggccgg aacttcggca gccggaacga cggcggcttc ggcctcgacg acttcctcct
     5401 cgcccttggt ggagacagac acgacgaccg gattctcctt gcccttcagc actgcggtca
     5461 cgccggccgg aagcttcaag tcgcgcacgc ggatcgtctc gtgcgcgcgc aggccggaca
     5521 ggtcgacctc gatgtactcg ggcaggtcct tcggcaggca ggcgacgtcg acatcggaca
     5581 gcacgtggct gatcagtccg cccgcttcct tgaccgccgg cgagttttcc tggccgacga
     5641 agtgcagggg cacccgcatg tggatcttct ggtcctcgga gacgcgctgg aaatcgatgt
     5701 gcagcacctg cggcttgtac gggtgcatct ggaagtcccg cagcagcacc cgctcgcccc
     5761 ggccgtcgag ttccatgtcg aggattgacg cgtggaactt ctctttgcgc agcgaatgga
     5821 acagcgggtt gtggtcgatc tcgatgttga cgggactgcc gttgccgcca tagatgatgc
     5881 ccggcacctt gttcgcgcgg cgcagggagc ggctcgcact cgtaccctgc ttggcgcgtg
     5941 tagttgcgac taccttcatt tcactctcca gttggggccc gcgggccgcg accagccacg
     6001 acgggacggt tactcgatga acagattgct caccgagtcc tcgcgcgata tgcgcgaaat
     6061 cgtttcaccc agcagcgccg cgcaggaaag ctgccggatc ttggggcact tctgcgcgtc
     6121 ctcgcgcagc gggatcgtgt cggtcaccac gacctcgtcg agcaccgaat tcatgatccg
     6181 ttcgaccgcc ttgcccgaca gcacggggtg ggtgcagtag gccatcacct tggccgcgcc
     6241 gtgatcgcgc aacgcagcgg ccgcgtggca cagcgtgttt gccgtgtcga tcatgtcgtc
     6301 catgatcacg caggtgcggc cggccacgtc gccgatgatg tgcatcacct cggcgacatt
     6361 cgcgcgcgga cggcgcttgt cgatgatcgc caggtccgca tccatgcgct tggccagcgc
     6421 gcgcgcgcgc accacgccgc cgacgtccgg cgaaaccacc atcaggttgt cgatgttgtg
     6481 cttccacagg tcgccgagca ggatcgggct ggcgtagatg ttgtcgaccg gaatgtcgaa
     6541 gaacccctgg atctggtctg cgtgcagatc catcgtcagc acgcgatgca cgcccaccgc
     6601 ctgcagcatg ttggcgacca ccttggcgct gattgccacg cgtgccgagc gtgggcggcg
     6661 gtcctgccgc gcgtagccga agtacggaat cgcggcggtg atacggccgg ccgagccgcg
     6721 cttgagcgcg tcgaccatga tcaccagttc catcaggttg tcgttggtcg gctggcaggt
     6781 cgactgcaga acgaacacgt ccttgccacg gacgttctcg ttgatttcga ccatcacctc
     6841 gccatcggag aagcgcgaca cggtggcgcg tccgagcgga atgttcagat gctggacgac
     6901 cgcgtgcgcg agcctcgggt tggcgttgcc cgtgaatacg accaggttct caggaaccat
     6961 cggcaccatc ttctcactgc gcacaaaagt cgcccgtcaa gtggccgact gctgcgttga
     7021 aaatggctgg ggaggaagga ttcgaaccct cgaatggcgg aatcaaaatc cgctgcctta
     7081 accagcttgg cgactcccca gcgcgaaacc tgcgcccgga tgatccggcg ccggatcacc
     7141 actccgcgag cggatgctgc ttcaggcccc tcacggacca ccccgtccac ccatccggca
     7201 accccgcaat cgccccccgc gcccgctcct cgctcgcggt ggcaacgaac accgctgaac
     7261 cggagcccgt catccgcgcc gtgccgaatc gtgcgagcag ggtcagcgcg gcgtcgacct
     7321 cgggaattcg gcgccgcacg accacttcga gatcgttggc gccgaacaaa gcgcgtggaa
     7381 actgcgcgag ccccgcggaa aaggcagaaa ttatcgtggc tttcgtatct cgggtcaaac
     7441 cggggtcacc gaagatttcg ctcgtcgcga ccgcggcttg cggcttcacc accgcgaacc
     7501 atgcggacgg cacttcgatc ggcgttaccc gctcgccgat gccttcgacg accgcggggc
     7561 cggtgccgag aaagaacgcg acgtcagcgc cgagtcgcgg cgccagcgcc gccaggtctg
     7621 cgcgcggcca gccgagccgc cacagccggt tcagcgcgat gagcgtggac gcggcgtcgg
     7681 aactgccgcc gcccatgccg ctgccggcgg ggattcgctt gtcgacacgg atcgcgacgc
     7741 cgagcttcgt gcccgactgc tgctgcagca ggcgcgcgga gcgcagcgcg agatcgcgct
     7801 gcggatcacc gatcacatcg ccgccgcgtg tgatcgcgcc gtcttcgcgc agttcgaagt
     7861 cgagcgtgtc ggccagatcg atcggcacga acacgctctg cagctcgtgg tagccatcgg
     7921 cgcgccggcc gagcacgtgg aggcaaaggt tcagctttgc cggcgcgggc acgccgagga
     7981 acaggcgcgt catctcggcc tcacgaggac ggatcgtcga gcacgagccg cactatgacg
     8041 gccggcgaac ccatggtggc agggcgctcg aacgagagtc ggcgcggcgc gccgtcgacg
     8101 ccgaagcggt cgagtacgtg gatcacccag ccatcctgct cgatgctgcc ggagtccaga
     8161 gtgcgatacg gccgcgacgg tgtcggccgg ccgacgatcc agtcaccgat gcccgacacg
     8221 ggcagcgacc acccgaggac ctgctccgcc agcgcgtcgg cgttcggtcc ctgcagccgt
     8281 ttcagttcgc cttcggcggc cggtgccgtc aggcgcgcac cgctgcgatc ggtatcgatc
     8341 cgtgccagcg tattgccaaa gggcgacgac agatcgagcg tgactccgtc cgcatggatc
     8401 gcgagcgaga agcggccgct gctgctttcg ctgcggtcgg gccaggtcgc ggtcaatgca
     8461 aagcgtcctt ggtgcacgcg ctcggcttcg cgcaccggcg cgagcgtcgt gcaggcgccg
     8521 agcagcacgc cagcgaccgc caatgccgcg gctgcccgca tcgcgcgtta cggcttgacc
     8581 ttgaagcgcg ccagcgtggc gcgcagcagc tcgttgtcgg gctctgcggc gagcgcttcg
     8641 cgccagacgc gacgtgcagc atcgcgatcg ccactgagcc aaagcacttc cccgaagtgg
     8701 gcaccgacct cgccttcggg ccgacccttc caagcccgtt cgagatactc gcgcgccttc
     8761 ggcaggtcgc ccagccgata gaagacccag cccatcgaat cgaggatgta gccgtcgttc
     8821 ggtgacagct tcagcgcctg tgcgatgagg tcgcgcgctt ccgccaggcg caggttgcga
     8881 tcggcgaagg tatagccaag ggcgttgtag gcgtgcgcgt cgtccggctt cagctgcatc
     8941 atgcggcgca gatgcgtttc catcaggtcg atgcgatcga gcttttcggc cgccatcgcc
     9001 gtgtcgtaca gaagattcgt gttgtcgggt gtcttcgcga gcgccgcggc cagcaggtcg
     9061 aaggactcgc gatagcgccg cgcttcgcgc agcagctggc cctcggcgac gaccacctgc
     9121 accttctcct catcgctgcg cgacggttgc gccgccaggt cggcgagcag acttcgcgct
     9181 tcgtcgacgc gcttcagctt gcccagcagc agcgcctgcc gcagacgcgc aggcaactgt
     9241 tgctcgccgt cgtcgatgcg ttcgagccac gcgattgcct cgtcgtactt gcgctcctcc
     9301 tcggcgatgc gcgcgaggtt catgtacgcc ggatcggggt cgcgggtgcc gggcgccgcc
     9361 tcgacggcct tcaggtagcg ctcgaagtac gcgcgcgatt cggtgcgcgg cggcttgccg
     9421 tcgagggtga gcacgcccat cgcgtacagc gcgtcggggt tgttcgcatc acgtttgagc
     9481 atgtcggcga actgctggcg cgcagccggc aggtcgttgt ccgcgatcag cagccgcgca
     9541 tacgtcaggc gcgcatcggc tgcctcggga cgccgctgca ggaagttccg cagcagttcc
     9601 agcgcctgtt tgcggccttc ggcgctgtcc ttgccgtcag ggcgcgccag gaattgcgct
     9661 gccgccaggc tggcgcgctc ggaatcgggc tgcagttcga ctgaagcgcg cgcctcctga
     9721 gccgcgcgct gcacgagtcc ggccgcatgc gcgccgctgg cgatgatcag gcggacgtcg
     9781 ccgccgtgcg cgggatcgct cagatacggc gccgccagtg actcgagcag ctggaacccg
     9841 ctggcgcgat ccggcccgcg cgctaacgtg cgctggacgc gtgccagttc gtcgaccgga
     9901 ctggcggcgc tcgcgatctg ctgcgccagc agtggtgcag cgtcctggta gcggccgttg
     9961 gcgatctgga tcgccgccag cgtctgcgcg gcctcgctcg aatgcggcgc cagttctcgc
    10021 cagagttgcg cggcttcgag cgcctgctcg gccgcgcgcg cgccgaacgc tatttcggcc
    10081 gcgcgccggg cgagccgtgg atcgtgcgtg gtgcgcgcaa cgttcatgta cgtggcgaat
    10141 gcggatcccg cgtcgccacg ctgcagcgcg acctcggcgg cgacgatctg atacagcaac
    10201 gcaccggtca ggtcgacatc gggcagggcg tcgagcctcg gtgccggcac cccctccgcc
    10261 ggcttgcccg gtccaacggg cgccgtcggt tgcgcaacgg acgtacaacc tacaatcgtg
    10321 ccgacgagca gcgccggcag tcccagtctg attctcaacg cccaatcctt cccagcagaa
    10381 ccctcaatac atcttagacg cgtcgacgat gcccgagttg ccggaggtgg aagtcacgcg
    10441 gcgcgcgctg gcgccgtacg tcgagggcga gcaggtgcag gcggtcatcg tgcgtgaccg
    10501 tcgcctgcgc tggccggtgc ccgcgcggct ggcggtcgcg ctgcaaggcc agcgcatcga
    10561 gcgccttgag cggcgcggca agtatctgct ttggcggttt gcgcatggca cgttgatctc
    10621 gcatctcggc atgtccgggt cctggcgtgt gcatcccggt acggtcccac cggctggcgc
    10681 gcacgaccat gtcgacatca tcgtggctgg gcgcccgcgt agcaccctgc gactgaccga
    10741 cccgcggcgg ttcggcgcgc tgctgtggca tccggtccgc cgcggcgggg cggagcgcca
    10801 tccgctgctg tcgcacctgg gggcggaacc gttcgatccg cgcttcgacg ccgaccacct
    10861 cttccaccac acgcgcaacc gccgctcgtc gatcaagcag gtactgctgg cgggcgatgt
    10921 cgttgtcggc gttggaaaca tctacgcgtc ggagagcctg tttcgcgccg gtatcgatcc
    10981 gcgcacggcg gcatttcgcc tgacgcgcgc gcgctgcgag cgcctcgtcg ctgcagtgcg
    11041 tgcggtgttg accgaagcga tcgaagtggg cggcagcacg ctgcggaact tcgtcggcgc
    11101 cgacggcgcc gagggctatt tcatgttgga ggcgtttgcc tacgaccgcg ccggtcagcc
    11161 ctgtcgggtc tgccgcacgc cgattcgacg catcttgcag gggcagcgtg ccacatactt
    11221 ttgcccgcat tgccagaccc gccgcgggcg tgtctgatcc ggcggactca tccgctcgaa
    11281 tggggggctg ggcgaaaacg ctgtgataga ttgaaacgcg acctagcgcc tcgtgcgcgt
    11341 cttccccgat ggcccaactc gcgagcagct tccaggaata cggcgcatgg cgtgattcgc
    11401 tggcgcacag cgtcacgcag cttcgcacct ggctgcaggg caaccagctg ctcgaccacg
    11461 acgcagcgcg ccggctcgcg ctcggcctcg accgcctcgc cgaggacaag ctcgtcatcg
    11521 cgttcgttgc cgagttctcg cgcggcaagt cggaactgat caacgccatc ttcttcgccg
    11581 actacggccg ccgcatcttg ccgtcgtcgg ccgggcgcac cacgatgtgc ccgaccgagc
    11641 tgatgtacga cgaatcgctg ccaccgtgca tccgcgcgct gccgatcgag acccgtgccc
    11701 ggcagggcac gatgtccgac ttcaagaagg cgggcgcgga gtggcgcgtg ttcccggtcg
    11761 atgtcgatgc gcccgagggg atgctgcagg cattcaggct cgtcgccgac acggtgcgcg
    11821 tatcggtgca ggaagccctg ctgtacggcc tgtacgaccc gtccgatccg gaccagcaga
    11881 cggcagtcgc ggcagacggc acgatcgaga tctcgcgctg gcgccatgcc gtgatcaact
    11941 tcccgcaccc actgctgaag cgcgggctgg tgatcctcga cacgccgggg ctgaacgcga
    12001 tcggcgccga gccggaattg acgctttcgc tggtgccgag cgcgcacgcg gttctgttcg
    12061 tgctggcggc cgacaccggc gtgaccagaa gtgacctcga cgtctggcgc caggtagtac
    12121 acgacaccgg tgcggcgacc aatcacatcg cggtgctgaa caagatcgac ggcctgtggg
    12181 acggtctgaa gaccgcgcac gagatcgacg gcgaggtcga tcgccaggtt caggcagtcg
    12241 gccacacgct cgaactcgac accgcgcgcg tgttcccggt ttcggcgcag aaaggtctcg
    12301 tcgcgaaggt catgcgcgac gagcggctgc tcgccgccag ccgtctgccg aaactggagt
    12361 cggccctggt cgacctcctg gttccggcca agcagcgcat cgtgggctca cagacgctcg
    12421 ccgtggtcga acgcatcggc gaagaggtcc ggcaggcgct ttccgcgcgc gagcgcggcg
    12481 tgatcgaaca gctgtacgag ctgcgcgctc tgcagggcaa gaaccagagc tcgatcgagc
    12541 gcatgtcgcg gcgggcgcag agcgagagcc gcgaattcga agccaacgtg cgcaagctcg
    12601 tggccacgcg tgttgtgctg acgcggttgt cggaccaggc gaccacgccg ctgtctcacg
    12661 aaacattgcg cacgacgctg gaggcaacac gcgagcgcat gcgcaaggtt cgcctgacgc
    12721 cgcagttcta ctcgtccata aaggactact tcgcggagtt gcagcagcac ctgcgcgacg
    12781 ccaacacccg gctcgccgag atcgaacaga tggtgatcgg cgtgcagcag cagtttgcat
    12841 ccgagctggg ctggagcctg ccgccgccga tgccgttcac gctcgacaag taccgcggcg
    12901 atctggagca ggtcgagcgc gcggcacgct cccacttcgg cgccttcgtc gtgatgacgc
    12961 gcgataagtg gtcgttgatc gagcgcttct tcgacaccgt ggtagcgcgc acgcgcgaga
    13021 tcttcactac cgccgagcgc gacacggaag cctggatccg ttcgctgctg ccaccaatcg
    13081 agtcgcaggt gcgcgagcag cgcgcgcagt tgaagaaacg cgccgagtcg gtcgagcgca
    13141 ttcgcaccgc tcaggaatcg ctcgattccc gcattggcga actggaagcc gcgctggagg
    13201 acacgcacgg caagctcgag gctttgcgcg ccggactcgc gcgcgtgcgg ctggtcggcg
    13261 cggcctgtcc ggtaacgcgg accgaagcgc cggatgccga tgccgccgcc atgccagtgc
    13321 gccgcaccga cccgaccgaa cccagctacc tgcgcgtaga aatctgatgc cgcgcttcgc
    13381 gcagcgcatc gttgcctggc agcgggagtt cgggcggcgc gatctgccgt ggcagcacac
    13441 gcgcgatccg tatcggatct ggctgtcgga aatcatgctg cagcagacgc aggtcgcgac
    13501 cgtcgcgccc tattacctgc gcttcgtcga gcggtttccg gatgtcgcgt cgctggcgcg
    13561 cgccgacgtg caggaggtcc tgcagctttg ggcggggctc ggctactacg cacgcgcgcg
    13621 caacctgcac gcctgcgcac ggcgtgtggt cgcggaacac ggcgggcggt ttccgtgcgg
    13681 cgcgattgcg ctcgcacaac tgcccggcat cggccgctcg accgcggcgg cgatcgcggc
    13741 cttctgcttc ggtgagcggg cggcgatcct cgacggcaac gtcaagcgcg tgctcgcgcg
    13801 gcacttcggc gtagaggggt ttcccggcgc ggcggcagtc gaacgcgaac tgtgggcgcg
    13861 cgccgatgca gtgctgccgg aagcgggcgg aatgccggca tacacgcagg gcctgatgga
    13921 tctcggcgcg acggtatgca cacgttccga tccgctctgc ggtcgatgcc cggtgcgcgc
    13981 aacctgcatt gcccggcaga ccaaccgtgt gcgggaattg ccggcgccac gtccgcggca
    14041 gccggtgcgt acgcgctcgg cgcacttgct gctgcttcag catgccgact gcattcttct
    14101 cgaacggcga ccgtatgccg gcatctgggg cggactgttg gcgccgccgc agttcgaccg
    14161 catcgaagac ctgtcgctcg cgctcgatca cctcgcgccc ggcacgaccg cgcgggcact
    14221 gccttcacgg cggcatgcgt tcacgcattt cacgctgcat ttcacgccgc atctggcgcg
    14281 tttcgaacat cccgtcgcag tgatggagcc gcgcctgcac tggcttgcac gcgcggacat
    14341 caagcaagca gcgctgccga cgccgatccg gaccctgctg cgcgacgtac tggcggctgg
    14401 ctgagtcgaa gagcgcgggg tcagcggatc accgcgtgct gccgcaggaa tcgctgcacg
    14461 atgtcgagcc gcgtgcgcgc gtcgtccagt tccatcagct tctgctttgc gcgcaacgaa
    14521 accggaagaa cctcgcacag ccggtttccg acccataccg aggagtgcag catatggggc
    14581 cgctcgatcg cgttcgcggc cagcatcccg gcgtcgtccg ggtggtcccg cagtgccgcc
    14641 tcgcgctgcg atttgagatc gtcgatgatg cgcgccagca gcgtcgcgca cggctgatgc
    14701 tcgggcgcga gcggtgcgtc gacatcggcc gcaatctcct cgacgtcacc gaccagcagc
    14761 ccgtccggtt gggccgcggc gtgccggacc cggaaccgct gcgtaccgag cgtgcggatg
    14821 ttcagcacgc cgagcttctg catgtccca
//
LOCUS       DBNHBY010000108         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1630042, whole genome shotgun sequence.
ACCESSION   DBNHBY010000108 DBNHBY010000000
VERSION     DBNHBY010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1630042"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /gene="glmS"
                     /locus_tag="AC16HB_02670"
     CDS             <1..1480
                     /gene="glmS"
                     /locus_tag="AC16HB_02670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5605722.1"
                     /translation="AAGYVFESQTDTEVIAHLIDQLYEGDLFDAVQRAVRQLHGAYAI
                     AVFCRDEPHRVVGARAGSPLVVGIGKQENFLASDAMALAGTTDQIIYLEEGDVVDLQL
                     ARVWIVDSAGRPAQRSVRTVAAFSNAVELGPYRHFMQKEIFEQPRAIGDTLEDVNEIV
                     PDLFGDGAADILERIDGVLILACGTSYYAGCVARYWLEAIAQVPVTVEIASEYRYRTS
                     VPNPNALVVVISQSGETADTLAALKHAKSLGMHRTLAVCNVATSAMVRETALQFLTRA
                     GVEIGVASTKAFTTQLVALYLLANAIAKAQGRLTPEREHAALKRLRHLPAAVGSVLAQ
                     EPHIIAVSEIFARKENALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLA
                     LVTDAMPVVTVAPNDALLEKLKSNMQEVRARGGQLFVFADADTHIESSEGVQVIRLPE
                     HYGDLSPLLHVVPLQLLAYHTAVARGTDVDKPRNLAKSVTVE"
BASE COUNT          278 a          507 c          548 g          242 t
ORIGIN      
        1 agcggcgggc tacgtgttcg aaagccagac ggacacggag gtcatcgcac acctgatcga
       61 tcaattgtac gagggcgatc tgttcgacgc cgtgcagcgc gcggtgaggc aactgcacgg
      121 cgcgtacgcg atcgccgtct tctgccgcga cgagccgcac cgcgtagtgg gcgcgcgcgc
      181 cggctcgccg ctcgtggtcg gtatcggcaa gcaggagaac ttccttgctt cggacgcgat
      241 ggcgctggcc ggcaccaccg accagatcat ctacctcgag gaaggcgatg tcgtcgacct
      301 gcaactggcg cgcgtgtgga tcgtggacag cgcaggacgt ccggcgcagc gatcggtgcg
      361 cacggtggca gccttctcca acgcggtcga gctgggtccc taccgccact tcatgcagaa
      421 ggagatcttc gagcagccgc gcgcgatcgg cgacacgctg gaagacgtca acgagatcgt
      481 cccggacctg ttcggcgacg gtgccgcgga catactggag cgcatcgacg gcgtgctgat
      541 cctcgcctgc ggtaccagct actacgccgg ctgcgtggcg cgctactggc tcgaggcgat
      601 cgcgcaagtg ccggtgacgg tggagatcgc cagcgagtac cgctaccgca cgtcggtgcc
      661 gaatccgaat gcgctggtgg tcgtgatctc gcagtccggc gaaaccgccg acacgctagc
      721 tgcgctcaag cacgcgaagt cgctcggcat gcatcgcacg ctcgcggtct gcaatgtcgc
      781 gacgagcgcg atggtgcgcg agaccgcgct gcagttcctg acccgcgcgg gcgtcgagat
      841 cggcgtcgcg tcgaccaagg cattcacgac gcagctcgtg gcgctgtacc tgctcgccaa
      901 cgcgattgcc aaggcgcagg gccggctcac acccgagcgc gagcacgcgg cgctgaagcg
      961 cctgcgccat ctgccggcgg cggtgggcag cgtgctggcg caggagccgc acatcatcgc
     1021 ggtgagcgag atctttgcgc gcaaggagaa cgcgctgttt ctcgggcgcg gcctgcatta
     1081 cccgatcgcg ctcgaaggtg cgctgaaact gaaggagatc agctacatcc acgccgaggc
     1141 ctacccggcg ggcgaactga agcacggccc gctggcgctg gtgaccgacg cgatgccagt
     1201 cgtcacggtg gcgccgaacg acgcgctgct ggagaagctg aagagcaaca tgcaggaagt
     1261 gcgtgcgcgc ggcggacagc tcttcgtgtt cgccgatgcc gacacgcaca tcgaatcgag
     1321 cgaaggcgtg caggtgatac gcctgcccga gcactacggc gatctgtcgc cgctgctgca
     1381 tgtcgtgccc ctgcagctgc tcgcgtacca cacggcggtc gcgcgtggga cggatgtcga
     1441 caaaccccga aatctggcga aatccgtgac ggtcgagtaa gcgactcgaa gcgggtgccg
     1501 agcccggcaa ccggtttcgg ggtttgcgga acaggctcac ttgccgcagg caagtcaggg
     1561 tggcgcagtc gccgg
//
LOCUS       DBNHBY010000109         4260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_73215, whole genome shotgun sequence.
ACCESSION   DBNHBY010000109 DBNHBY010000000
VERSION     DBNHBY010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_73215"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="AC16HB_02675"
     CDS             complement(<1..625)
                     /locus_tag="AC16HB_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5605723.1"
                     /translation="MKPRDLVDLLLLAALWGAAFLFMRMAAPVFGPVALVEVRVAIAS
                     ACLLALLAWRGGLQRLHVHALPLLMVGVLHTAVPFVLFSYSVLSITGGFASILNATTP
                     MWTALVASLWLRERFRGAQWLGLAIGLAGVAVLVWGKVDFHPAGSQWSGTLAILAGLA
                     GAASYGVAGNYTKRHLAGVSSLAVATGSQIGAALVLIPFALALWPHGA"
     gene            complement(622..1092)
                     /locus_tag="AC16HB_02680"
     CDS             complement(622..1092)
                     /locus_tag="AC16HB_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HIR5605724.1"
                     /translation="MSFRIVALAPDPFVELGRRSDAELARRHAHRMVADESDSYPCRV
                     SLRYAQAGEELLLLNFAHQDADSPYRARGPIFVRVGAPGLQLAPGEVPSLVRRALLSV
                     RGYTRAGWIEFAAVTAGRAVAESIARAFARAGVDYLHLHFARPGCFLCRVDRVQ"
     gene            complement(1113..1595)
                     /locus_tag="AC16HB_02685"
     CDS             complement(1113..1595)
                     /locus_tag="AC16HB_02685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5605725.1"
                     /translation="MRYTVCASPIGPLTLVSEGTCLAAVRFARRNGAPDIGRNWQRDD
                     TLPVLAQARAQLDEYFAGKRTRFDVPLKLDGTEFQQAVWRALQKVGFGRTSTYGALAA
                     AVGRPDAARAVGAVMGANPIPIIVPCHRIIGADGTLTGFGGGIDRKTRLLQLEGVLLA
                     "
     gene            complement(1607..3052)
                     /gene="alkA"
                     /locus_tag="AC16HB_02690"
     CDS             complement(1607..3052)
                     /gene="alkA"
                     /locus_tag="AC16HB_02690"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HIR5605726.1"
                     /translation="MQIDADACYRAVRARDARFDGRFFTAVRTTRIYCRPVCTAPLPK
                     RENCSFFPSAAAAEDAGFRPCLRCRPELAPGLASVDAAARLARAAANLIDDGALAEAS
                     LASLATRLGVTDRHLRRVFEAEFGVTPVAYAQTARLLLAKRLLTDTAMPVTEVALAAG
                     FGSLRRLNTLFAQRYRLSPTVLRAQRAQARPLDHHVFRLHTRAPYDFDALLRFLATRA
                     IDGVEAIDDRGYRRTLALQNAQGRVACGWVEVRRAPRGTGVEARIDARLASVIPRVLA
                     AVKHTFDLNCRPEEVAAALGELAHARPGLRLPGAFDAFEIGVRGILGQQITVKAARTL
                     AARLVQRFGAPLDTPFAGVERVFPAVATLAECKPSQIAELGVIAQRAHSIVALARALA
                     DGALQLAPGVDVERTLDALRQLPGIGPWTAHYIALRALHWPDAFPPGDVGVLRALRVT
                     RAAEAEARAQAWRPWRGYAVIHLWQSREKST"
     gene            complement(3113..4189)
                     /gene="aroG"
                     /locus_tag="AC16HB_02695"
     CDS             complement(3113..4189)
                     /gene="aroG"
                     /locus_tag="AC16HB_02695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HIR5605727.1"
                     /translation="MRYMTDDLRIKEIKELVPPAHLLREFPCTDTASQTVFEARSAAH
                     RVLHAMDDRLLVVIGPCSIHDPKAALEYAERLAEQRKRLASELEIIMRVYFEKPRTTV
                     GWKGLINDPDLDGSFRINHGLRVARELLWEINEKALPAGCEYLDMITPQYIADFVAWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNVRIAAEAIRAAQQPHHFLSVTKGGHS
                     AIVSTKGNEDCHVILRGGRAPNYDSASVEAACQELARNGLAQRVMIDASHANSRKQHD
                     NQVAVCADIAQQLEAGEARIIGVMIESHLVGGRQDLVAGKPLNYGQSITDACLGWEET
                     VPALERLSAAVRTRRLRAANYSGK"
BASE COUNT          662 a         1512 c         1477 g          609 t
ORIGIN      
        1 ttgccccgtg cggccatagc gcgagcgcga acgggatcag gaccagcgcc gcgccgatct
       61 gactgccggt cgcgaccgcg agcgaactca cgccggcgag atgccgcttc gtgtaattgc
      121 ccgcgacacc gtacgacgcg gcgccggcga gcccggccag gatcgcgagc gtgcccgacc
      181 actgcgagcc ggccgggtga aagtcgacct tgccccacac cagcaccgca acgccggcaa
      241 gcccgatcgc caatcccagc cactgcgcgc cgcggaagcg ttcgcgcagc cacagcgatg
      301 cgaccagcgc cgtccacatc ggcgtggtcg cgttcaggat cgacgcgaag ccgccggtga
      361 tgctgagcac cgaatacgag aacagcacga agggcacggc cgtatgcagc acgccgacca
      421 tcaggagcgg aagcgcgtgc acatgcaagc gttgcagccc gccgcgccac gcgagcaacg
      481 ccagcaggca cgcgcttgcg atggccacgc gcacctcgac cagcgcgacc ggcccgaata
      541 ccggtgcggc catgcgcatg aacagaaacg ccgcgcccca cagcgcagcg agcagcaaca
      601 gatcgacgag gtcgcgcggc ttcattgcac gcgatcgacg cggcacagga agcagccggg
      661 ccgcgcgaag tgcaggtgca ggtaatcgac gccggcgcgg gcgaacgcgc gcgcgatcga
      721 ttctgcgacg gcgcggcctg cggtcactgc ggcgaattcg atccagcccg ccctcgtata
      781 gccgcgcacc gacagcagcg cacggcgcac gagcgacggc acctcgccgg gtgcgagctg
      841 cagccccggc gcgccgacgc gaacgaagat cggaccgcgc gcgcggtacg gcgagtccgc
      901 gtcctggtgc gcgaaattca gcagcagcag ttcttcgccg gcctgcgcat agcgcaggct
      961 gacgcggcac ggatagctgt cggactcgtc ggcgaccatt cgatgggcat ggcggcgcgc
     1021 gagttcggcg tcgctgcgtc ggcctaactc gacgaacggg tccggcgcca gagccacgat
     1081 tcggaacgac aagcgagact ccctttcgac gttcaggcga gcagcacgcc ctcgagctgc
     1141 agcagcctcg tcttgcgatc gatcccgccg ccgaagccgg tcagcgttcc gtccgctccg
     1201 atgatgcgat ggcatggcac gatgatcggg atcggattcg cccccatcac cgcgccgacc
     1261 gcgcgcgccg cgtcgggccg gccgacggcg gccgcgagcg caccgtaggt cgacgtgcgg
     1321 ccgaagccga ccttctgcag cgcgcgccac accgcctgct ggaactcggt gccgtccagc
     1381 ttcagcggca cgtcgaagcg cgtgcgcttg cctgcgaaat actcgtcaag ctgtgcgcgc
     1441 gcctgcgcca ggaccggaag ggtgtcgtcg cgctgccagt tgcgcccgat gtccggcgcg
     1501 ccgttgcggc gcgcgaagcg caccgcggcg aggcaagtgc cttcggagac gagtgtcagc
     1561 ggcccgatcg ggctcgcgca gaccgtgtag cgcatcgtgc tgttcctcat gtcgatttct
     1621 cccgcgattg ccatagatga atgacggcgt acccgcgcca cgggcgccac gcctgcgcgc
     1681 gcgcttcggc ctccgccgcg cgcgtgaccc ggagcgcgcg cagcacgccg acgtcgccgg
     1741 gtggaaacgc gtcgggccag tgcagcgcgc gcagcgcgat gtagtgcgcc gtccatggcc
     1801 cgatgccggg cagctggcgc agggcgtcga gcgtgcgctc gacgtcgacc ccgggcgcaa
     1861 gctgcagtgc cccgtcggcg agcgcgcgcg cgagtgcgac gatcgaatgc gcgcgctgcg
     1921 cgatcacgcc cagttctgca atctggctcg gcttgcattc ggccagcgtg gcgacggcgg
     1981 gaaacacgcg ctccacaccg gcaaacggcg tgtcgagcgg cgcgccgaag cgttgcacga
     2041 gccgcgctgc gagcgtgcgc gccgccttca ccgtgatctg ctggcccagg atcccgcgca
     2101 cgccgatctc gaacgcgtcg aacgctcccg gcagtcgcaa ccccggccgc gcgtgcgcga
     2161 gttcgccgag cgcagcggcc acttcctccg gacgacagtt caggtcgaag gtgtgcttga
     2221 ccgccgccag cacgcgcggg atgacggacg cgagccgcgc atcgatgcgc gcctccacgc
     2281 ccgtgccgcg cggcgcgcgc cgcacttcga cccagccgca cgcgacgcgg ccctgtgcgt
     2341 tctgcagcgc cagcgtgcgc cggtagccgc gatcgtcgat cgcctcgacg ccgtcgatcg
     2401 cgcgcgtcgc cagaaaccgc agcagggcgt cgaagtcata cggcgcgcgc gtgtgcagac
     2461 ggaacacatg gtggtcgagc gggcgagcct gggcgcgctg tgcccgcagc accgtcgggc
     2521 tcagccgata gcgttgcgcg aacagcgtat tgagccggcg caggctgccg aatccggctg
     2581 ccagcgcgac ctccgtcacc ggcattgcgg tgtcggtcag cagtcgcttt gcgagcagca
     2641 gccgcgcggt ctgcgcgtat gccaccggcg tcacgccgaa ctcggcctcg aacacgcgtc
     2701 gcaggtggcg gtcggtgacg ccgagccgtg tcgccagcga cgcgaggctc gcctccgcga
     2761 gcgcgccgtc gtcgatcagg ttggcagcag cacgcgccag ccgggccgca gcgtcgacgc
     2821 tcgccagccc cggcgccagt tccggacggc agcgcaggca gggccggaag ccagcatcct
     2881 cggccgccgc ggcgctcggg aagaagctgc agttctcgcg cttgggcaac ggcgcggtgc
     2941 acaccggccg gcagtagatg cgcgtcgtgc gcaccgcggt gaagaagcgg ccgtcgaagc
     3001 gcgcgtcgcg ggcgcgaacg gcgcggtagc aggcgtcggc atcgatttgc acgccggcac
     3061 tatggccgaa aatggcgcct cctgctcgcc gttttcggac atcggcacgc tgctacttgc
     3121 cggagtagtt cgccgcccgc agccggcgcg tgcgcaccgc ggcggagagc cgctcgagtg
     3181 ccggaaccgt ctcctcccag ccgaggcagg cgtcggtgat cgactgcccg tagttcagcg
     3241 gtttgccggc gacgagatcc tgccggccgc cgaccagatg gctttcgatc atcacgccga
     3301 tgatgcgcgc ctcgcctgcc tcgagttgct gcgcgatgtc tgcgcagacg gcgacctgat
     3361 tgtcgtgctg cttgcggctg ttcgcgtggc tggcgtcgat catcacgcgc tgcgccaggc
     3421 cgttgcgcgc cagttcctgg cacgcagcct ccacgctcgc tgagtcgtag ttcggcgcgc
     3481 ggccgccgcg caggatcacg tggcagtcct cgttaccctt ggtcgaaacg atcgcggagt
     3541 gcccgccttt ggtgaccgac aggaaatggt gcggctgctg ggcggcgcgg atcgcctcgg
     3601 cggcgatgcg cacgttgccg tcggtgccgt tcttgaagcc gaccgggcac gacaggcccg
     3661 acgcgagttc gcggtgcacc tgcgattcgg tcgtgcgtgc gccgatcgcg ccccaggcga
     3721 cgaaatccgc gatgtattgc ggcgtgatca tgtcgaggta ctcgcagccg gccggcagcg
     3781 ccttctcgtt gatctcccac agcaactcgc gcgccacgcg cagcccgtgg ttgatgcgga
     3841 agctgccgtc cagatccggg tcgttgatca ggcccttcca gccgacggtg gtgcgcggtt
     3901 tctcgaagta gacacgcatg atgatttcca gctccgacgc cagccgcttg cgctgctccg
     3961 ccaggcgctc ggcgtattcg agcgcagcct tcggatcgtg gatcgaacat ggcccgatca
     4021 cgaccagcag ccggtcatcc atcgcgtgca ggacgcggtg cgcggcgctg cgcgcctcga
     4081 acactgtctg cgacgcggtg tcggtgcatg ggaactcgcg caacaggtgc gccggcggga
     4141 cgagttcctt gatctccttg atgcggagat cgtcagtcat gtaacgcatc ggtcgggctc
     4201 catccatggc agggtttccg ttgtttcggc ccgcccggcg aaaaaaaacc gccaggtttc
//
LOCUS       DBNHBY010000110         3483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1020803, whole genome shotgun sequence.
ACCESSION   DBNHBY010000110 DBNHBY010000000
VERSION     DBNHBY010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1020803"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="AC16HB_02700"
     CDS             complement(<1..1120)
                     /locus_tag="AC16HB_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605728.1"
                     /translation="MNPSRQFILRPVATTLLMVAILVAGLVAYRFLPQSALPQVDYPT
                     IQVTTLYPGASPEVVTSSITAPLERQFGQMPGLSQMWSTSSAGASVITLRFDLALTLD
                     VAEQEVQAAINAAGTFLPNDLPQPPVYNKVNPADAPILTLGLTSKTLPLTRVQDLADT
                     RLAQKLSQVPGVGLVSVSGGQKPAVRVQVNPKALAALGLSLDDLRAAIAAGNVNQAKG
                     SFDGPTRASTIDANDQLKSEDDYRDLVVAWKNGAPIRVRDVADVVNDAENVRLAAWVN
                     ADPGIVLNIQRQPGANVIETVDRIKALLPQLAQGLPQAIEIKPLTDRTVTIRASVADV
                     QLELLFAVALVVLAIFLFLRSVPATIIPSLAVPLSLVGT"
     gene            complement(1117..2421)
                     /locus_tag="AC16HB_02705"
     CDS             complement(1117..2421)
                     /locus_tag="AC16HB_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HIR5605729.1"
                     /translation="MRDDAPLEDSPQTGVPDRSGAARGSRRRFAAIIAVVVIAAALAI
                     WYALRPGPTATAPATSAASGKGGRFDPAGRPMPVSAVAARAADLPLRLTALGTVVARN
                     TVTVRSRVDGQLLRVHFREGDTVKAGQLLAQIDPRPFEVALAQAEGQQARDRAQFDGA
                     KVDLARYEGLLATDSIARQQVDTQQALVRQLDAALAVDKAAADNARLQLSYTRIAAPV
                     SGRVGLRQVDPGNLVHAGDANGLAVVTQVQPIDVVFPLPQDNVPAVLARLRSGSTLRV
                     DAFDREGSKRLATGTLMTADNLADPATGTVKMKAEFDNKDSALFPNQFVNVRLLLDTL
                     KNATVVPTSAIQRGAPGTYVYVVRDDSTVTVRKVTLGPADGETTAISDGLAVGERVVS
                     DGADKLREGARVEVIDRNAQPAAAPAPRDGKGERKRRDATKP"
     gene            2642..>3483
                     /locus_tag="AC16HB_02710"
     CDS             2642..>3483
                     /locus_tag="AC16HB_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5605730.1"
                     /translation="MQYVYTMRRVGKIVPPKREILKNISLSFFPGAKIGVLGLNGAGK
                     STLLRIMAGEDKNYEGEAVPMPGMKVGFLPQEPQLDPEKTVRQEVEAGLGEVFDAKAK
                     LDAIYAAYAEPDADFDALAAEQARYEAILATRGEDLEHQLEIAADALRLPPWDAKIAS
                     LSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVEWLEQFLLRFPGTVVGVTHDRY
                     FLDNACQWILELDRGFGIPYEGNYSAWLEQKEKRLEIEAKQEQARIKTMKAELEWVRQ
                     NAKGR"
BASE COUNT          539 a         1206 c         1209 g          529 t
ORIGIN      
        1 aggtgccgac cagcgacagc ggcaccgcga ggctcgggat gatcgtcgcc ggcacgctgc
       61 gcaggaacag gaagatcgcc agcacgacca gcgccaccgc gaacagcagt tcgagctgta
      121 cgtcggcgac cgacgcgcgg atcgtcaccg tgcggtccgt gagcggcttg atctcgatcg
      181 cctgcggcag tccctgcgcg agctgcggca gcaacgcctt gatgcgatcg acggtctcga
      241 tcacgttcgc gccgggctgg cgctgaatgt tcagcacgat gcccggatcg gcattgaccc
      301 acgccgccag tcggacgttc tcggcgtcgt tgaccacgtc ggcgacgtcg cgcacgcgga
      361 tcggcgcacc gttcttccac gcgaccacga ggtcgcggta atcgtcttcc gacttcagct
      421 ggtcgttggc gtcgatggtc gaggcgcgcg tcggcccgtc gaagctgccc ttggcctggt
      481 tgacgttgcc ggccgcgatc gccgcgcgca ggtcgtcgag cgacagcccg agcgccgcca
      541 gcgccttcgg gttgacctgc acccgcaccg cgggcttctg tccgccgctg acgctgacca
      601 gccccacgcc cggcacctgc gacagcttct gcgcgagccg cgtgtcggcg aggtcctgca
      661 cgcgggtgag cggcagcgtc ttcgaggtca ggcccagcgt caggatcggc gcgtcggccg
      721 gattgacctt gttgtacacg ggcggttgcg gcagatcgtt cggcaggaac gtgcccgcgg
      781 cattgatcgc ggcctgcact tcctgctccg ccacgtcgag cgtcagcgcg agatcgaagc
      841 gcagcgtgat caccgaggca ccggccgaac tcgtcgacca catctgcgac agcccgggca
      901 tctgtccgaa ctggcgctcc agcggcgcgg tgatcgacga cgtgaccacc tcggggctcg
      961 cgcccggata cagcgtggtg acctggatcg tcgggtagtc gacctgcggc agcgccgact
     1021 gcggcaggaa gcgatacgca accagaccgg cgacgaggat cgcgaccatc agcagcgtgg
     1081 tcgccaccgg gcgcaggatg aactggcgcg acggattcat ggcttggtgg cgtcgcgccg
     1141 cttgcgttcg cccttgccgt cgcgcggcgc aggcgcagcg gctggctgcg cattgcggtc
     1201 gatcacctcg acgcgcgcgc cttcgcgcag cttgtcggcg ccgtcggaca ccacgcgctc
     1261 gccgaccgcg agcccgtcgc tgatcgcggt cgtctcgccg tcggcgggcc cgagcgtgac
     1321 cttgcgcacg gtgaccgtcg aatcgtcgcg caccacgtag acgtaggtgc ccggcgcgcc
     1381 gcgctggatg gcgctggtcg gcacgacggt cgcgttctta agcgtgtcga gcagcagccg
     1441 cacgttgacg aactggttcg gaaacagcgc gctgtccttg ttgtcgaact cggctttcat
     1501 cttcacggtc ccggtggccg ggtcggcgag gttgtcggcc gtcatcagcg tgccggtcgc
     1561 cagccgcttg ctgccctcgc ggtcgaacgc atcgacccgc aacgtgctgc cgctgcgcag
     1621 gcgcgccagc accgccggca cgttgtcctg cggcagagga aacacgacgt cgatgggctg
     1681 gacctgcgtg acgacggcga gcccgttggc gtcgccggca tggaccagat tgcccggatc
     1741 gacctggcgc agtccgacgc gccccgacac cggcgccgcg atgcgcgtat acgagagctg
     1801 cagccgcgcg ttgtccgccg ccgccttgtc gaccgcgagc gccgcatcga gctggcgcac
     1861 cagcgcctgt tgcgtgtcga cctgctggcg cgcgatcgaa tcggtcgcga gcagcccttc
     1921 gtagcgcgcc agatcgacct tggcgccgtc gaactgcgcg cgatcgcgcg cctgctgccc
     1981 ttcggcctgc gcgagcgcca cttcgaacgg tcgcgggtcg atctgcgcca gcagttgccc
     2041 cgccttcacc gtgtcgcctt cgcggaagtg cacgcgcaag agctggccgt cgacgcggct
     2101 gcgcaccgtc accgtattgc gcgcgacgac cgtgccgagc gcggtcagcc gcagcggcag
     2161 gtcggcggcg cgcgccgcga ccgcgctgac cggcatcggc cgtccggcgg gatcgaaccg
     2221 gccgcctttg cctgatgccg cggacgtcgc cggggcggtc gcagtcggac ctggccgcag
     2281 cgcataccag attgctagag cggcggcgat cacgaccacg gcgatgatcg cggcgaagcg
     2341 gcgccgcgaa ccgcgcgcgg cgccggatcg atccggaacg ccggtctggg gggagtcttc
     2401 gaggggagcg tcgtctctca tggcaggtcg gcccgagcca tgctcggtga agcacggcaa
     2461 caggcgtcaa ttctcctctc tggcgggccg ggcgccgggc tcggccagga gatggccaga
     2521 ccctaagccg tgcatgtaaa tgctttgccg ccgattcttt gcaaaacgta tccgccgccc
     2581 tgcgataatc ctgcgttgca accattccgc accgacggcg ccgcagcgcg ccgctcttcc
     2641 catgcaatac gtgtacacca tgcgccgggt cggcaagatc gtgccgccga agcgcgagat
     2701 cctgaagaac atctcgctga gtttctttcc gggcgccaag atcggcgtgc tggggctcaa
     2761 cggcgccggc aagtcgacgc tgctgcggat catggccggc gaggacaaga actacgaggg
     2821 cgaggcggtg ccgatgccgg gcatgaaagt cggcttcctg ccgcaggagc cgcagctcga
     2881 cccggagaag accgtgcgcc aggaagtcga ggcggggctg ggcgaggtgt tcgacgcgaa
     2941 agccaagctc gacgcgatct acgccgccta cgcggagccc gatgccgact tcgacgcgct
     3001 cgccgccgag caggccaggt acgaagccat cctcgccacc cgcggcgagg acctcgagca
     3061 tcaactggag atcgccgccg acgcgctgcg actgccgccg tgggacgcga agatcgcgag
     3121 tctctccggc ggcgagaagc gccgcgtcgc gctgtgtcgc ctcctgctgt cgaagcccga
     3181 catgctgctg ctggacgagc cgaccaacca cctcgacgcc gagagcgtcg agtggctgga
     3241 gcagttcctg ctgcgctttc cgggcacggt cgtcggcgtc acgcacgacc gctacttcct
     3301 cgacaacgcg tgccagtgga tcctcgaact cgaccgcggc ttcggcatcc cgtacgaggg
     3361 caactactcg gcgtggctgg agcagaagga aaagcggctg gagatcgagg ccaagcagga
     3421 gcaggcgcgc atcaagacga tgaaggccga gctcgaatgg gtgcgccaga acgccaaggg
     3481 ccg
//
LOCUS       DBNHBY010000111         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1697847, whole genome shotgun sequence.
ACCESSION   DBNHBY010000111 DBNHBY010000000
VERSION     DBNHBY010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1697847"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16HB_02715"
     CDS             complement(<1..304)
                     /locus_tag="AC16HB_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5605731.1"
                     /translation="MGFLAGKRILITGLLSNRSIAYGIAQACRREGAELALTYVGERF
                     KERVTEFAQEFGSSIVLACDVGEDAQIASMVEGLRAHWDGLDGLVHSIGFAPREAIS"
     gene            complement(386..1924)
                     /locus_tag="AC16HB_02720"
     CDS             complement(386..1924)
                     /locus_tag="AC16HB_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5605732.1"
                     /translation="MALPSFPRFDTRTLACALLACALAPFAARADDPAPPTELTVTSA
                     TTSAAATTPDPLGQLLRKLESSHPAESARVEPLATGDTSIDVWERIRRGFQMPDLTGR
                     LADKSTRWYAAQPDYIGRMSSRASRYLFHIVEEIEKRGMPTELALLPFVESAMQPEAE
                     SHAKAAGLWQFIPSTGKLYQLEQNLWKDERRDVLESTRAALDYLQKLYNDFGDWHLAL
                     AAYNCGEGCVAKAIARNRAQRRLLNYSSLRLPKETQYYVPKLQAIKNIVRAPQDYGIA
                     LPAIQNEPYFAVVRKTRDMDIATAARLAEMPLDEFQALNPSFNRPVMVGAAQSRILLP
                     ADHADTFIGNLAAYEATGQALASWTTYAMKPNETLAQVAERAGISEAALREANRIPPR
                     FRLAAGSTILLPRDETMEGDIAADKLDAAFALVPEQPNLRRITYRVRRGDTLHSVARR
                     WRVPVDDVVAWNRLRSRDLFAGQRLNLMVAATAAPKKNTVAQKKGQSRNGKKKTQVAA
                     SGRR"
     gene            complement(2006..>2317)
                     /locus_tag="AC16HB_02725"
     CDS             complement(2006..>2317)
                     /locus_tag="AC16HB_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5605733.1"
                     /translation="KLAALPAATRMYCAHEYTLANIRFALAVEPGNAALQARQRACEA
                     LRAQGKPTLPSTLADELATNPFLRCDVAAVRTRAERESPGANASTVAAFAALRAWKNR
                     F"
BASE COUNT          325 a          764 c          826 g          402 t
ORIGIN      
        1 cgctgattgc ctcgcgcggg gcgaacccga tcgaatgcac cagcccgtcg agaccgtccc
       61 aatgtgcgcg cagcccctcg accatcgacg cgatctgtgc gtcctcgccg acgtcgcacg
      121 cgagcacgat cgaggagccg aactcctgcg cgaactcggt cacgcgctcc ttgaagcgct
      181 cgccgacgta ggtgagggcg agttcagcgc cttcgcgccg gcacgcctgc gcgatgccgt
      241 aggcaatcga tcgattcgac agcagcccgg tgatcaggat gcgtttgccg gccaggaatc
      301 ccatgatttt tcggtcgagc aggagttgcg gtcggatgcg gatgctaccg gcccaccgcc
      361 gccggcagag gttcgggcgc cggcgtcagc gccggccgga cgcggcgacc tgtgttttct
      421 tcttgccgtt gcgcgattgg cccttcttct gggcgactgt gttcttcttc ggcgcggccg
      481 tggcggcgac catcaggttc agccgctggc cggcaaacaa gtcgcgcgaa cgcagccggt
      541 tccacgcgac cacgtcgtcg accggcacgc gccagcggcg cgccaccgag tgcagcgtgt
      601 cgccgcgccg cacgcgatag gtgatacggc gcaggttcgg ctgctcgggc accagtgcga
      661 aggcggcgtc gagcttgtcg gccgcgatgt cgccttccat cgtctcgtcg cgtggaagca
      721 ggatcgtcga accggctgcg agtcggaacc tgggcggaat gcgattggcc tcgcgcagcg
      781 ccgcttcact gatgccggcg cgctccgcga cctgggccag tgtctcgttc ggtttcatcg
      841 catatgtggt ccagcttgcc agcgcctgtc cggtcgcctc gtaggccgcc agattgccga
      901 tgaaggtgtc ggcgtggtcg gcgggcaaca gaatgcgcga ctgggccgcg cccaccatga
      961 ccggccggtt gaacgacggg ttgagcgcct ggaattcgtc cagcggcatt tccgccagcc
     1021 gcgcggcggt ggcgatgtcc atgtcgcgcg ttttgcgcac gaccgcgaaa tacggctcgt
     1081 tctggatcgc cggcagcgcg atgccgtagt cctgcggcgc gcgcacgatg ttcttgatcg
     1141 cctgcagctt cggcacgtag tactgggttt ccttcggcag gcgcaggctc gaatagttga
     1201 gcagccggcg ctgcgcgcga ttgcgcgcga tcgccttggc gacgcaacct tcgccgcagt
     1261 tgtaggcggc cagcgccagg tgccagtcgc cgaaatcgtt gtacagcttc tgcaggtagt
     1321 cgagcgcggc gcgggtggat tcgagcacgt cgcgccgctc gtccttccat aggttctgct
     1381 ccagctgata cagcttgccg gtcgaaggaa tgaactgcca cagcccggcc gccttggcgt
     1441 gcgactcggc ctccggctgc atcgcgcttt ccacgaaggg cagtagcgcg agttcggtcg
     1501 gcatgccgcg cttctcgatt tcctcgacga tgtggaacag gtagcggctg gcgcggctcg
     1561 acatgcggcc aatgtaatcc ggttgcgccg cgtaccagcg cgtgctcttg tcggccagcc
     1621 ggccggtgag gtcgggcatc tggaaaccgc ggcggatccg ctcccacacg tcgatcgacg
     1681 tgtcgcccgt cgcgagcggc tccacgcgcg ccgactcggc gggatggctg gattcgagct
     1741 tgcgcagcag ttggccgagc ggatcgggtg tggtggctgc cgcgctggtg gtcgcgctcg
     1801 tcaccgtcag ctcggtcggc ggcgccggat cgtcggcgcg tgccgcgaac ggcgcaagcg
     1861 cgcacgcgag cagtgcgcac gccagcgtgc gggtgtcgaa gcgcgggaat gagggcagcg
     1921 ccatccggaa ccgtcgcttg ccggcgtgaa aggtcgcggc agtatatagg cgcgacttcg
     1981 gcacgggggc ggcgcgatcc atcggtcaga accggttctt ccaggcgcgc agcgctgcga
     2041 atgcggcgac cgtcgacgcg ttcgcgccgg ggctttcgcg ctcggcgcgc gtccgcaccg
     2101 cggcgacgtc acagcgcagg aacggattgg tcgccagctc gtccgcgagc gtcgacggca
     2161 gcgtcggctt gccctgcgcg cgcagcgcct cacacgcgcg ctggcgggcc tgcagcgcgg
     2221 cgttgccggg ctcgaccgcg agcgcgaagc gaatgttggc gagcgtgtat tcgtgtgcgc
     2281 agtacatgcg cgtcgcggcg ggaagggcgg ccagctt
//
LOCUS       DBNHBY010000112         3249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1630134, whole genome shotgun sequence.
ACCESSION   DBNHBY010000112 DBNHBY010000000
VERSION     DBNHBY010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1630134"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="AC16HB_02730"
     CDS             complement(<1..540)
                     /locus_tag="AC16HB_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5605734.1"
                     /translation="MTSSFSAADLEAHIPRLRRYARALTGNREAADDLTQDTLERAWG
                     KRTLWHAGEGDPAGSLRAWLFAVMHNVFVNSVRRMKPVESLDAGGDSVPEVAASGASA
                     ETGAAVRDIRKAMLRLPDEQREVILLVGLEQLSYAEAAYALDVPIGTVMSRLSRGRER
                     LRLMLEGDADQEPRVGAPST"
     gene            complement(693..2717)
                     /locus_tag="AC16HB_02735"
     CDS             complement(693..2717)
                     /locus_tag="AC16HB_02735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR5605735.1"
                     /translation="MPSAMYSQIIVDLPIEQPLDYENGTDAPAPTIGQLCVVPVGRRR
                     TVGICVGTSSSSTVDIGRVRPVARIIDEIPPLASNWRELTRFAADYYQHNWGEVAIPA
                     LPPQLRSPPGPRLVSVLARLRKWEFGLEGQPVSSVVRLNSEQLAAASAILERPGFAPN
                     LLFGVTGSGKTAVYLESIARVLERETDSQCLLLVPEINLTPQLLAPVALRFPEVRVVA
                     MHSAMSDRDRSAAWLAAHERRARVVVGTRLAVFASLPQLRLIVVDEEHDASYKAGEGV
                     RFSARDLAIKRAQIEAIPVVLGSATPSLETWAHAQSGRYRLLRLAERVEARAHPSIEL
                     IDIRRAPPESGISDALGQALSDVVNAGEQALVFINRRGYAPVLACAACGWLSSCRHCS
                     VYAAFHKADGTLRCHHCGWQKRVPRACPSCGNQDLGAVGLGTQRIEEGLRRLLPNARI
                     ARIDRDSTRRRGAAESAFAAVHAGETDVLVGTQMIAKGHDFQRVALVGVLNVDAQLLS
                     PDFRAPERLFATLMQVAGRAGRAGRSSRVLVQTRYPQHPLLGAFARQDYEGFADLQLA
                     ERRAAGMPPFVSQALCTADARQLESALAFLAGARRLGQRKGGAVRIYDPVPMPVVKIG
                     GLHRAQLLVEADQRLDLQSFLRAWLAEVRSAAKETRPRARWRIEVDPLEI"
     gene            complement(3020..>3249)
                     /locus_tag="AC16HB_02740"
     CDS             complement(3020..>3249)
                     /locus_tag="AC16HB_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5605736.1"
                     /translation="QGNLDPMVLFAGDAAVRKAARATLDNFGPVQRPDGRWGGHVFNL
                     GHGISQFTPPDAVKVLVDTVHSESRSFRRTN"
BASE COUNT          595 a         1101 c         1022 g          531 t
ORIGIN      
        1 agtcgaaggc gcacccacgc gcggctcctg atctgcatcg ccctcgagca tcagacgcag
       61 ccgctcgcgg ccccgcgaga gccgcgacat cacggttccg atcgggacgt cgagcgcata
      121 cgccgcctcg gcgtatgaga gttgctccag gcccaccaac agaatcactt cccgctgttc
      181 atccggcaac cgcagcattg ccttgcggat gtcacgcact gccgcgccgg tctccgcgct
      241 agcgccgctc gccgccacct cgggcaccga gtcgccgccc gcgtcgagcg attcgaccgg
      301 cttcatccgc cgcacggaat tgacgaacac gttatgcatc accgcaaaga gccacgcgcg
      361 caggctgccg gccggatccc cctcgcctgc gtgccagagc gtccgcttgc cccatgcacg
      421 ctcgagtgtg tcctgtgtca ggtcatcggc cgcctcgcgg ttgccggtca gcgcgcgcgc
      481 atatcgccgc agacgtggaa tgtgcgcctc gagatccgcc gcgctgaagg aggatgtcat
      541 cgatcgaccg gccgactcac acgatcaaca tcatcgaaac gctccgatcc cagcggccca
      601 catccctgcg cgtcatcggc gtttccttgc gacccgatca gcaaaacgga agcggacacg
      661 actttattcc gtctcgcgcc tggcgcgcgg actcagatct ccagcggatc gacctcgatg
      721 cgccagcgcg cgcgcggcct ggtctctttc gcggcgctgc gcacttcggc gagccacgcc
      781 ctgaggaatg actgcagatc gaggcgctga tccgcctcga cgagcagttg tgcccgatgc
      841 agtccgccga tcttgacgac cggcatcggc accggatcgt agattcgaac ggcgccgcct
      901 ttgcgctgac cgagtcggcg cgctccggcg aggaacgcca gggccgactc gagttgccgc
      961 gcgtcggccg tgcacagtgc ttgcgacaca aagggtggca tgccggccgc gcgacgttca
     1021 gccagttgca ggtcggcgaa tccctcgtag tcctgacgcg caaacgcgcc cagcaagggg
     1081 tgctgcggat agcgtgtctg caccagcacg cgactcgagc gccccgcacg gccggctcgc
     1141 cctgccacct gcatcagcgt tgcgaacaag cgctcgggag cgcgaaagtc gggcgacagc
     1201 aactgtgcat cgacgttgag aacgcctacc agtgcgacgc gctggaaatc gtgccccttg
     1261 gcaatcatct gcgtgccaac cagcacatcg gtctcgccgg catggaccgc ggcgaacgcg
     1321 gattcggccg cgccacgccg ccgggtcgag tcgcgatcga tgcgcgcaat gcgcgcgttt
     1381 ggcagcaggc gccgcagtcc ttcctcgatg cgctgcgtgc ccagtccgac tgcaccgagg
     1441 tcctgattgc cgcagctcgg acacgctcgt ggaactcttt tctgccagcc gcagtggtga
     1501 caacgcaggg ttccgtcggc cttgtgaaac gccgcgtaga cggaacagtg acgacaactc
     1561 gatagccacc cgcacgcggc gcatgcgagc accggcgcgt agccgcgacg gttgatgaag
     1621 acgagcgcct gctcgcctgc gttgaccacg tcgctgagcg cttgcccgag ggcatccgag
     1681 attccgctct ccggcggcgc tcgacggatg tcgatcagct cgatcgacgg gtgcgctcgt
     1741 gcttcgacgc gctcagccag ccgcagcagg cgatagcggc ccgactgcgc atgtgcccag
     1801 gtttccagcg acggcgtcgc cgatccgagt acgaccggaa tggcctcgat ttgcgcgcgc
     1861 ttgatcgcga ggtcgcgggc ggaaaaccgc acgccttcgc ccgccttgta ggacgcgtcg
     1921 tgctcctcgt cgaccacgat cagtcgcagt tgcggcagcg atgcaaagac ggcgagccga
     1981 gttccgacca cgacgcgggc gcgtcgctcg tgcgcggcca gccacgcggc cgaacgatcg
     2041 cgatcgctca tggcgctgtg catggcaacg acacgaacct cgggaaaccg aagcgccacc
     2101 ggcgccagca gctgcggggt caggttgatc tccggtacca gcagcaggca ctgcgaatcg
     2161 gtctcgcgct cgagcacgcg cgcgatcgac tcgaggtaga ccgccgtctt cccgctgccg
     2221 gtgacaccga agaggagatt cggagcgaag cctggccgct cgagaatcgc cgatgcagcg
     2281 gcgagctgct ccgagttcag tctgacaacg ctcgaaaccg gctgcccttc aaggccgaac
     2341 tcccacttgc gcaggcgagc cagtactgaa acgagtcgtg gccccggggg ggaccgcaac
     2401 tgcggcggca gggccgggat ggcgacctcg ccccagttgt gctggtagta gtccgccgcg
     2461 aagcgcgtca gttcacgcca gttcgatgcc agtggaggga tctcgtcgat gattctcgcg
     2521 acaggtcgaa cccgaccaat gtcgaccgtg ctcgaggacg acgtgccaac gcatatcccg
     2581 acagtccgcc ggcgcccgac cggtacgacg caaagctgcc cgatcgtcgg cgcaggcgcg
     2641 tcggtgccat tttcgtagtc gagcggttgc tcgatcggca ggtcaacgat gatctgcgag
     2701 tacatcgcgg acggcacgct cgatgatctt ggaaggcact tcaagtagag agcgcaagta
     2761 cctgattcat ttgttcgaat ccaggcgcat cgatcctgtg gaaaactttg tggacaagct
     2821 gaccgcgccc tctgggcaca cgatccaagg gctgcagcgg cggaacgtcg ggttacccgc
     2881 tgccgaggta aagagttttg taatcagaga cttggagtca actcgtcagt gcccgcacac
     2941 tcggcaaagg ccctgatttc acgcctatac cgagaacgcg cgtttgtgaa taagtcaagc
     3001 ccagtcgcga aaacgcgact cagttcgtgc gcctgaacga gcggctttcg gagtgaactg
     3061 tgtccaccag aactttgact gcgtccggtg gcgtgaactg cgaaatccca tgcccaagat
     3121 tgaagacgtg gccaccccaa cgcccatcgg gccgctgaac aggcccgaag ttatccaagg
     3181 tggcacgagc ggccttgcgc actgctgcgt cgcccgcgaa gagaaccatg ggatcgaggt
     3241 ttccctgca
//
LOCUS       DBNHBY010000113         2382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1697972, whole genome shotgun sequence.
ACCESSION   DBNHBY010000113 DBNHBY010000000
VERSION     DBNHBY010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1697972"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16HB_02745"
     CDS             complement(<1..523)
                     /locus_tag="AC16HB_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605737.1"
                     /translation="MMRPLPTRWFEIVVARDDAFIALEALAGAGCVEIEWHPAAGQVD
                     PARNAELLKDFGDLARRYRPYWPAAATRLAAERRAPLDAMAEALQTLARWSAAADGAI
                     AELQRAEGLLRELRLTEDALRAMTDARFDFSQLAQASHGVIASVFALPPRAPLAVPQD
                     VLMREALLANERLV"
     gene            complement(520..1302)
                     /locus_tag="AC16HB_02750"
     CDS             complement(520..1302)
                     /locus_tag="AC16HB_02750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5605738.1"
                     /translation="MISGLPLDFAHARIGAHLSRRPDDRVWSQARSARTVAALLESLR
                     ASPAAGDVSGIAPRADSDDIERAFRQQLRLRIAEVARWAPQEWRAALLWTRHLVDLPA
                     LIQLLGDEPPAAWMRADPVLADHARATPLQRRIALADSELAPVARALAAGGETAPARL
                     HVAVEAWRREWRRRWPRAGAEVRAQLEALERAVAEHVVRFAGVPAEQTTETRSALATR
                     ALRFVHRFAGQPVALFAYLLVAAIDLERLRGEFALRAAMVRP"
     gene            complement(1299..1652)
                     /locus_tag="AC16HB_02755"
     CDS             complement(1299..1652)
                     /locus_tag="AC16HB_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605739.1"
                     /translation="MSTETVRNTAETSAVDAAIEQVLKAEQAARDAVNRCGADAELRV
                     AQAREQARRIAERAAERIARVQDIAQARLQARMGAIERERAALDRPETAASAQVAHLR
                     AAIDRLAAELTSEAT"
     gene            1806..2105
                     /locus_tag="AC16HB_02760"
     CDS             1806..2105
                     /locus_tag="AC16HB_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605740.1"
                     /translation="MKKLLIALTLGSSLVLASGIAAAQDAKKGEAAIKEAGCLTCHDV
                     AKKKMGPAFKTAAADLKKAGATTPDKVIATIKSKHAEVKAKDDDLKNIAAWLLTL"
BASE COUNT          357 a          866 c          806 g          353 t
ORIGIN      
        1 gcaccagccg ctcgttcgcg agcagcgcct cgcgcatcag cacgtcctgc ggcaccgcca
       61 gcggcgcgcg cggcggcagc gcgaacacgc tcgcgatcac gccgtggctc gcctgcgcga
      121 gctgcgagaa atcgaaacgc gcgtcggtca tggcgcgcag cgcgtcctcg gtcagacgga
      181 gttcgcgcag cagcccctcg gcacgctgca gttccgcgat cgcgccgtcg gccgctgcgc
      241 tccaccgtgc cagcgtctgc agcgcctctg ccatcgcatc gagcggcgca cgccgctcgg
      301 ccgcgagccg cgttgccgcg gccggccagt aggggcggta tctgcgcgcg agatcgccga
      361 agtccttcag cagttcggcg ttgcgcgccg ggtcgacctg gcccgcggcc ggatgccatt
      421 cgatctccac gcagcccgcg ccggccagcg cctcgagcgc gatgaaggcg tcgtcgcgcg
      481 cgaccacgat ctcgaaccag cgcgtgggca gcgggcgcat catggccgca ccattgccgc
      541 gcgcagcgcg aactcgccgc gcagccgctc caggtcgatc gccgccacca gcaggtacgc
      601 gaagagcgcc accggctgcc ctgcaaagcg gtgcacgaag cgcagcgcgc gggtcgccag
      661 tgcgcttcgc gtttcggtgg tctgctcggc cggcacgccg gcgaagcgga cgacatgctc
      721 ggccaccgcg cgttcgagcg cctcgagttg cgcgcgcact tcggccccgg cgcgcggcca
      781 gcgccggcgc cattcgcggc gccacgcttc caccgccacg tgcagtcttg ccggcgcggt
      841 ttccccgccg gcggccagcg cccgcgcgac cggcgccagt tcgctgtcgg ccagcgcgat
      901 gcgccgctgc aggggcgtgg cacgcgcatg gtcggccagg acggggtcgg cgcgcatcca
      961 cgccgccggc ggctcgtcgc cgagcaattg aatcagcgcc ggcaggtcga cgaggtgccg
     1021 ggtccacagc agcgccgccc gccattcctg cggcgcccag cgtgcgacct cggcaatgcg
     1081 cagccgcagt tgctgccgga aggcgcgctc gatgtcgtcg ctatccgcgc gcggggcgat
     1141 gccggacacg tcgccggccg ccggcgaggc gcgcagagat tccagcagcg ccgccaccgt
     1201 gcgcgccgaa cgcgcctgcg accacacgcg gtcatcgggg cgccgcgaca gatgcgcacc
     1261 gatacgcgcg tgcgcgaagt cgagcggcag tcccgaaatc atgtcgcttc cgaggtcagc
     1321 tcggccgcaa gccggtcgat cgcggcacgc aggtgcgcga cctgcgcgct ggcggccgtt
     1381 tccggtcgat cgagcgccgc gcgctcgcgt tcgattgcgc ccattcgtgc ctgcaggcgc
     1441 gcctgcgcga tgtcctgcac gcgggcgatg cgctccgccg cgcgctcggc gatgcggcgc
     1501 gcctgctcgc gcgcctgcgc gacgcgcagt tcggcgtcgg cgccgcaccg attcaccgcg
     1561 tcgcgcgccg cctgctctgc tttcagcacc tgctcgatgg cggcgtccac cgctgacgtt
     1621 tccgcggtat tgcgcaccgt ttcggtcgac atggagcctt tcctcgcgcc ccgtcagtgt
     1681 cgcgtgcggt tcaggcccat ggttgacggc tgtcaagtgc ttcatgttgc gtcaacggca
     1741 cagtcgccgg cgcgttgttg gatcgtggtc atcaacgatc accaacccat ccggaagggg
     1801 acctcatgaa gaaactgctc attgcactga cactcggaag ctcactagtg ctggcaagcg
     1861 gcatcgccgc cgcgcaggac gccaagaagg gcgaggccgc gatcaaggag gcgggctgcc
     1921 tgacctgcca cgacgtggcc aagaagaaga tgggcccggc attcaagacc gccgccgcgg
     1981 acctgaagaa agccggcgcc accacgcccg acaaggtcat tgcgacgatc aaatccaagc
     2041 acgccgaagt gaaggcgaaa gacgacgacc tgaagaacat cgctgcgtgg ttgctgacgc
     2101 tgtaacgggt cacgtaacat gttttcctaa aggccggcac cgccggcctt tttttgttct
     2161 tcttcaggac gccggctgcg actggggaag gaccggcgcg agtgtcatcc ggcacggcgt
     2221 gcgcggccgc atgtaacgat ttccgtatca catggcgcat gtcgcgcgaa cacgacgcgc
     2281 acggatcgcc ttgctcgcca tatgatgagc gcaggttcgg ggcacatcga acctgtgtcg
     2341 ctcgcaatcc gcagtttcct tcgcacgaac cggccacttt tt
//
LOCUS       DBNHBY010000114         7170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1630206, whole genome shotgun sequence.
ACCESSION   DBNHBY010000114 DBNHBY010000000
VERSION     DBNHBY010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1630206"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..304)
                     /locus_tag="AC16HB_02765"
     CDS             complement(65..304)
                     /locus_tag="AC16HB_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6967 family protein"
                     /protein_id="HIR5605741.1"
                     /translation="MEEKLRATEIAKLRAPIGGQEIELQQIDYVHGGLSLLRIRIREG
                     RRFTVFDIDSATAATWGEAMLNWSRSQAPAQGGTP"
     gene            479..1774
                     /gene="dsrA"
                     /locus_tag="AC16HB_02770"
     CDS             479..1774
                     /gene="dsrA"
                     /locus_tag="AC16HB_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HIR5605742.1"
                     /translation="MAKPMHETPMLNELESGPWPSFVTGLKRLAKDKDYMVDVLGQLE
                     TSYETRKGYWKGGTVSVFGYGGGVIPRFTELKDEKGNPKFPDAAEFHTLRVMPPAGMH
                     YSTEVLRKMCDIWEQHGSGLIAFHGQSGDIMFQGVKSDKVQDAFDAINELGFDLGGAG
                     PAVRTSMSCVGSARCEQSCYDEGKAHRTVINSFLDDIHRPALPYKFKFKFSGGPNDCM
                     NSVQRADLAVIGTWRDNIKTDEALAKKWFARHGMTELVNDVIARCPTKAIQIKEIKDV
                     RKGESISSVAVNDTQALEIDNRDCVRCMHCINVMTGALAHGDDTGATILCGGKRTLKI
                     GDLMGTVIVPFMKLKTDEDREAFVDLGKRIVDFFAENALEHERIGETIERIGLVNFLE
                     GVGLEVDPNMVAAPRTNPYVRTDGWDEEVAKMQAKKKAA"
     gene            1786..2892
                     /gene="dsrB"
                     /locus_tag="AC16HB_02775"
     CDS             1786..2892
                     /gene="dsrB"
                     /locus_tag="AC16HB_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HIR5605743.1"
                     /translation="MAQVEAPVKPAAPKTPRRAVESGVPDNTPFLHPLMKKNYGKWKW
                     HDRPRPGVLHHVAHSGDELWTVKAGTQRQMDVWTIRKLCDIIDKFGEGHVRFTIRSNV
                     EIGVSKWDKVEPLIKALESAGFPVGGTGNSVSMIAHTQGWLHCDIPGTDASGAVKALM
                     DELIHEFRHEEMPNRVHMSTSCCEINCGGQADIAIIVQHTKPPKINHDLVANVCERPA
                     VVARCPVAAIRPALVNGKPSLEVDEKKCICCGACYPPCPPMQINDPEHSKLAIWVGGK
                     NSNARGKPTFMKMVASGLPNNPPRWPEVSEVVKRILRVYKEDARSWERMADWIERIGW
                     PRFFEKTGLTFTKYLIDDWRGSRANLNASAHIRF"
     gene            2907..3266
                     /gene="tusD"
                     /locus_tag="AC16HB_02780"
     CDS             2907..3266
                     /gene="tusD"
                     /locus_tag="AC16HB_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HIR5605744.1"
                     /translation="MKLAVLVNEGPYQHQASDSAYLFCQAALAKGHELLRVFFYHDGV
                     NNASKFTEPPQDDRNIVARWSKFAEEHKVDLVVCVAAALRRGIVADSLAPGFRISGLG
                     QLIDAGIKADRLVTFGD"
     gene            3270..3629
                     /gene="tusC"
                     /locus_tag="AC16HB_02785"
     CDS             3270..3629
                     /gene="tusC"
                     /locus_tag="AC16HB_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HIR5605745.1"
                     /translation="MAKKFMLINRKAPYGSIYALESLEVVLIAAAFEQDVSLVFADDG
                     VYQLKKGMQTKAIATKNFSPTYRALEDYDVDKLYVEAESLAARGLSAEDLVTDVKVVD
                     AKTLAGLMEQQDVVLSF"
     gene            3639..3935
                     /gene="tusB"
                     /locus_tag="AC16HB_02790"
     CDS             3639..3935
                     /gene="tusB"
                     /locus_tag="AC16HB_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HIR5605746.1"
                     /translation="MLHIVNKSPLEKPMLDSVLRLAQSGALLLIEDGVYAATRGNKAE
                     SKLRDVMGKLKVYALAPDLQARGMADRLIDGVATVDYAGFVDLVTEHPNCQSWL"
     gene            3962..4297
                     /locus_tag="AC16HB_02795"
     CDS             3962..4297
                     /locus_tag="AC16HB_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR5605747.1"
                     /translation="MPTIEVSGKSYETDEEGYLVNLGDWNEDVANAIAGAENIEMSEN
                     HWEVVNFLRKYYEEYQIAPAVRVLTKAIGKTLGPEKGNSQYLYELFPYGPAKQACKIA
                     GLPKPTGCV"
     gene            4362..5075
                     /locus_tag="AC16HB_02800"
     CDS             4362..5075
                     /locus_tag="AC16HB_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR5605748.1"
                     /translation="MFALLFYAATVVLVAGLVARIADYARTPAPLKIPTTPAPTTRGG
                     VVLRMAREVVLFESLFKGSLWTWLFGWVFHLSLALVLVRHLRYFTEPVWFWVAFAQPL
                     GILAAFGMLIGLAGLLARRVLVERIRYISVPSDYLMLVLLILIAASGLALKYLFHTDI
                     VAVKAFFLGLLRFDWQPLPADGALYVHLSLVLALLVIFPFSKLLHAPGVFFSPSRNMA
                     DNPRETRHLAPWAAKLERR"
     gene            5076..6608
                     /gene="dsrK"
                     /locus_tag="AC16HB_02805"
     CDS             5076..6608
                     /gene="dsrK"
                     /locus_tag="AC16HB_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrK"
                     /protein_id="HIR5605749.1"
                     /translation="MAAEVTAPPLREFPSPPALVPGAMEKSRPYVANEKMQQAIGFPG
                     QLVDGWESRAIEHMGSLLGKYRSLKVFMDSCVHCGACADKCHYFIGTQDPKNMPVARQ
                     ELMRSVYRRYFTLPGKLFPKLVGARDLTRDVLDEWYTYFNQCSECRRCSVFCPYGIDT
                     AEVTMAAKEILDVVGYGQKYTNEIIGKVHKVGNNLGLPGPALADTLSGLEEDVKEDTG
                     ADVRFPLDEPGAEVLLVTPSADFFAEPHVDSLIGYAKVLHAAGIRWTLSTKASEAGNF
                     GLFIGNYEQLRAIALRIREAALELGVKRIVVGECGHAWRVAYSFWNTLVGIGAGGRDP
                     FAIQLQRQLDPSVRQPTHICEFTWDLIQRGALKFDKEANDHRVITFHDSCNVARASRM
                     GDLPGGQFEIPRAIIRAVANKYVEMAPDTTRERTFCCGGGGGLLTDELLDLRVKGALP
                     RMEALRAVADQHGVNFMATICAICKAQFTKVLPYYGFKMNMVGGVHQLVSTAIKLDAA
                     PH"
     gene            6627..>7170
                     /locus_tag="AC16HB_02810"
     CDS             6627..>7170
                     /locus_tag="AC16HB_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5605750.1"
                     /translation="MSSPAQTDAQPLTFRRFKDGDFKWKDLHQKIFSADHSHKCPTYI
                     QSTPPCQGSCPSGEDIRGYLNIVRGMEKPPAGMSWQEYAWRRLTEANPFPSIMGRVCP
                     APCETGCNRNEVEDHVGINSVEHYLGEWAIKNELKFNAPARRSGKSVAVIGGGPAGLS
                     CAYQLALKGHDVTIFDEREYL"
BASE COUNT         1324 a         2296 c         2394 g         1156 t
ORIGIN      
        1 ccgtacgagt aatcgacatc gatcgtgtcg cccgcaatcg cgaacgcgaa gtcgatcgag
       61 gcggtcacgg cgtgccgcct tgcgccggcg cctgcgaccg gctccagttc agcatcgctt
      121 cgccccatgt cgcggccgtg gccgagtcga tgtcgaacac ggtgaaacgg cggccctcgc
      181 ggatgcggat gcgcagcaag ctcagcccgc cgtgcacgta gtcgatctgc tgcagctcga
      241 tctcctggcc gccgatcggc gcgcgcagct tggcgatctc ggtggcgcgc agtttctctt
      301 ccatgcagtc gacgctttct ccctgaatgc acgggcattc tgcccgcgtt cgagatgccg
      361 ttgcgggctg cggccgcgca cccctccctt tggggttatg cgcgtaatca ccgcgggtga
      421 tggttcggcc ccggcacggc ccgtacgatg cccgcggaca cctgcgtcag gaggactcat
      481 ggcaaagccg atgcacgaaa cgccgatgct caacgagctc gaaagcggtc cgtggccgag
      541 cttcgtgacg gggctgaaac gcctcgccaa ggacaaggac tacatggtcg acgtgctcgg
      601 ccagctcgaa acctcgtatg aaacccgcaa gggctactgg aagggtggca cggtcagcgt
      661 gttcggctac ggtggtggcg tgatcccgcg cttcaccgag ctgaaggacg aaaagggcaa
      721 tccgaagttc ccggacgcgg ccgagttcca cacgctgcgc gtgatgccgc cggcgggcat
      781 gcactactcg accgaggtgc tgcgcaagat gtgcgacatc tgggaacagc acggctcggg
      841 cctgatcgcg ttccacggcc agagcggcga catcatgttc cagggcgtga agtcggacaa
      901 ggtgcaggac gccttcgacg ccatcaacga actgggcttc gatctcggcg gcgcgggtcc
      961 ggcggtgcgc acgtcgatgt cgtgcgtcgg aagcgcgcgc tgcgagcaat cgtgctacga
     1021 cgagggcaag gcgcaccgca cggtcatcaa cagcttcctc gacgacatcc atcgcccggc
     1081 gctgccgtac aaattcaagt tcaagttctc cggcggcccg aacgactgca tgaactccgt
     1141 gcagcgcgcg gacctggcgg tgatcggcac ctggcgcgac aacatcaaga ccgacgaggc
     1201 gctggcgaag aagtggttcg ccaggcacgg catgaccgaa ctggtgaacg acgtcatcgc
     1261 gcgctgcccg accaaggcga tccagatcaa ggaaatcaag gacgtccgca agggtgagtc
     1321 gatttccagc gtggccgtca acgacacgca ggcgctcgag atcgacaacc gcgactgcgt
     1381 ccgctgcatg cattgcatca acgtgatgac gggcgcgctc gcgcacggcg acgacaccgg
     1441 cgccacgatc ctgtgcggcg gcaagcgcac gctgaagatc ggcgacctga tgggcacggt
     1501 gatcgtgccc ttcatgaagc tcaagaccga cgaggaccgc gaggcgttcg tcgacctcgg
     1561 caagcgcatc gtcgacttct tcgccgagaa cgcgctcgag cacgagcgca tcggcgagac
     1621 gatcgagcgc atcgggctgg tcaatttcct cgaaggcgtg ggcctcgagg tcgatcccaa
     1681 catggtcgcg gcgccgcgca ccaatccgta cgtgcgcacc gacggctggg acgaggaagt
     1741 ggccaagatg caggccaaga agaaagcggc ttgagaggaa tgacgatggc gcaagtcgaa
     1801 gcaccagtga aacccgcggc gccgaagacg ccgcggcggg cggtcgaaag cggcgtgccg
     1861 gacaacacgc cgttcctgca cccgctgatg aagaagaact acggcaagtg gaagtggcac
     1921 gaccggccgc gtccgggcgt gctgcaccac gtggcccatt ccggcgacga gctatggacg
     1981 gtcaaggccg gcacgcagcg gcagatggac gtgtggacca tccgcaagct gtgcgacatc
     2041 atcgacaagt tcggcgaagg ccatgtgcgc ttcacgatcc gcagcaacgt cgagatcggc
     2101 gtgtcgaagt gggacaaggt cgagccgctg atcaaggcgc tcgaatcggc cggcttcccg
     2161 gtcggcggca ccggcaactc ggtgtcgatg atcgcgcaca cgcagggctg gctgcactgc
     2221 gacatcccgg gcaccgacgc gtcgggcgcg gtcaaggcgc tgatggacga actgatccac
     2281 gagttccgcc acgaggagat gccgaaccgc gtgcacatgt cgacctcgtg ctgcgagatc
     2341 aactgcggcg gccaggccga catcgcgatc atcgttcagc acaccaagcc gccgaagatc
     2401 aaccacgacc tcgtcgccaa cgtgtgcgag cggccggcgg tggtggcgcg ctgcccggtg
     2461 gccgccatcc ggccggcgct ggtcaacggc aagccctcgc tcgaagtcga cgagaaaaag
     2521 tgcatctgct gcggcgcctg ctacccgccg tgcccgccga tgcagatcaa cgatcccgag
     2581 cactccaagc tcgcgatctg ggtcggaggc aagaactcca acgcgcgcgg caagccgacc
     2641 ttcatgaaga tggtcgcgtc gggcctgccg aacaacccgc cgcgctggcc cgaggtgagc
     2701 gaagtcgtca agcgcatcct gcgcgtctac aaggaagacg cgcgctcgtg ggagcgcatg
     2761 gccgactgga tcgagcgcat cggctggccg cgcttcttcg agaagacggg gctcaccttc
     2821 accaagtacc tgatcgatga ctggcgcggc tcgcgtgcga acctgaacgc gtcggcgcac
     2881 atccggttct agcgacgcga gggaacgtga agcttgcagt cctggtcaac gaaggcccgt
     2941 accagcacca ggccagcgat tccgcctacc tgttctgcca ggcggcgctg gccaaggggc
     3001 acgaactcct gcgcgtgttc ttctatcacg acggcgtgaa caacgcgtcg aaattcaccg
     3061 agccgccgca ggacgaccgc aacatcgtcg cgcgctggag caagttcgcc gaggagcaca
     3121 aggtcgacct cgtcgtgtgc gtggcggcgg ccctgcggcg cggcatcgtc gccgactcgc
     3181 tcgcgccggg gttccggatc tccggcctcg ggcagctgat cgatgccggc atcaaggccg
     3241 atcgtctcgt gacgtttggg gattgagcga tggccaagaa attcatgctg atcaatcgca
     3301 aggcgccgta cgggtcgatc tatgcgctcg aaagcctgga ggtcgtgctg atcgccgccg
     3361 ccttcgagca ggacgtgtca ctggtgttcg ccgacgacgg cgtgtatcag ctgaagaagg
     3421 gcatgcagac caaggcgatt gcgaccaaga acttctcgcc gacgtatcgc gcgctggagg
     3481 actacgacgt cgacaagctg tacgtcgagg ccgagtcgct cgccgcgcgc gggctgagcg
     3541 ccgaggacct ggtcacggac gtgaaggtcg tcgacgcgaa gacgctcgcc ggcctgatgg
     3601 agcagcagga cgtggtactg agcttctagg aaaccgagat gctgcacatc gtcaacaagt
     3661 caccgctcga aaagccgatg cttgattcgg tgctgcggct ggcgcagtcc ggcgcgctgc
     3721 tgctgatcga ggacggcgtg tatgcggcca cgcgtggcaa caaggccgag tcgaagctgc
     3781 gcgacgtgat ggggaagctg aaggtctacg cgctcgcgcc tgacctgcag gcgcgcggca
     3841 tggcggaccg cctgatcgac ggcgtggcga cggtcgacta cgcgggcttc gtcgacctgg
     3901 tcaccgagca cccgaactgt caatcctggc tgtaggaatt catttcaaag aggagcatca
     3961 catgccgacc atcgaagtca gcggcaagag ttacgagacc gacgaggagg gttacctcgt
     4021 caatctcggc gactggaacg aggacgtcgc caacgccatc gccggtgccg agaacatcga
     4081 gatgtccgag aaccactggg aagtggtgaa cttcctgcgc aagtactacg aggaatacca
     4141 gatcgccccg gccgttcgcg tgctgaccaa ggcgatcggc aagacgctcg gaccggagaa
     4201 gggcaacagc cagtacctgt acgagctgtt cccgtacggt ccggccaagc aggcgtgcaa
     4261 gatcgcgggc ctgccgaagc cgaccggctg cgtttaacgg tcggggatcc gggtggcgcg
     4321 tagcgccacc cggtacttgt cctatgtcca ctgctggcat cgtgttcgcg ctgctgttct
     4381 acgcggcgac cgtggtgctg gtcgccgggc tcgtggcgcg catcgccgat tacgcgcgca
     4441 cgccggcgcc gctgaagatt ccgaccacgc ccgcgccgac cacgcgcggc ggggtcgtgc
     4501 tgcgcatggc gcgtgaggtg gtgctgttcg aaagcctgtt caagggaagc ctgtggacgt
     4561 ggctgttcgg ctgggtcttc cacctgagcc tcgcgctggt gctggtgcgc cacctgcgct
     4621 acttcactga accggtgtgg ttctgggtgg cattcgcgca gccgctcggc atcctcgcgg
     4681 cgttcggcat gctgatcggg ctggcgggcc tgctggcgcg gcgcgtgctg gtcgaacgca
     4741 tccgctacat ctcggtgcca tcggactatc tgatgctggt gctgctgatc ctgatcgcgg
     4801 cctccggcct cgcgctgaag tacttgttcc ataccgacat cgtggcggtg aaggcattct
     4861 tcctcggcct gctgcgcttc gactggcagc cgctgcccgc ggacggcgcg ctgtacgtgc
     4921 acctgtcgct ggtgctggcg ctgctggtga tcttcccgtt ctcgaagctt ttgcatgcgc
     4981 ccggcgtgtt cttctcgcct tcgcgcaaca tggccgacaa tccgcgcgag acgcggcacc
     5041 tggcgccgtg ggcggccaaa ctggagcgca gatagatggc cgccgaagtc accgcaccgc
     5101 cgttgcgcga attcccgtcg ccgcccgcgc tcgtgcccgg cgcgatggag aaatcgcggc
     5161 cctacgtcgc gaacgagaag atgcagcagg cgatcggctt tccgggccag ctcgtggacg
     5221 gctgggagag ccgggcgatc gagcacatgg gctcgctgct cggcaagtac cggtcgctca
     5281 aggtcttcat ggattcgtgc gtgcactgcg gcgcgtgcgc ggacaagtgc cactacttca
     5341 tcggcacgca ggacccgaag aacatgccgg tcgcgcgcca ggaactgatg cgaagcgtct
     5401 accgtcgcta tttcacgctg ccgggcaagc tgttcccgaa gctcgtcggc gcgcgcgacc
     5461 tgacccgcga cgtgcttgac gagtggtaca cgtacttcaa ccagtgctcc gagtgccgcc
     5521 gctgctcggt gttctgcccg tacggcatcg ataccgccga agtgaccatg gcggcgaagg
     5581 agatcctcga cgtcgtcggc tacggccaga agtacacgaa cgagatcatc ggcaaggtgc
     5641 acaaggtcgg caacaacctc gggcttccgg gcccggcact ggccgacacg ctgagcgggc
     5701 tcgaagagga cgtcaaggaa gacaccggtg ccgacgtgcg ttttccgctc gacgagccgg
     5761 gcgccgaggt gctgctggtc acgccgagcg ccgatttctt cgccgagccc cacgtcgaca
     5821 gcctgatcgg ctacgcgaag gtgttgcacg ctgccggcat ccgctggacg ctgtcgacga
     5881 aggcgtccga ggctggcaac ttcggtctgt tcatcgggaa ctatgaacag ttgcgcgcga
     5941 tcgcgctgcg catccgcgaa gcggcgctcg aactgggcgt gaaacgcatc gtcgtcggcg
     6001 agtgcggcca cgcatggcgc gtcgcataca gcttctggaa cacgttggtc ggcatcggcg
     6061 ccggtggtcg ggacccgttc gcgatccagc tgcagcgcca gctcgacccg agcgtgcgcc
     6121 agccgacgca catctgcgaa ttcacctggg acctgatcca gcgcggagcg ttgaagttcg
     6181 acaaggaagc gaacgatcac cgcgtgatca cgttccacga ctcgtgcaac gtcgcgcgcg
     6241 cttcgcgcat gggcgaccta ccgggcggcc agttcgagat cccgcgcgcg atcatccgcg
     6301 cggtcgcgaa caagtacgtc gagatggcgc ccgacaccac gcgcgaaagg accttctgct
     6361 gcggcggcgg cgggggactc ctgaccgacg aactgctcga cctgcgcgtc aagggcgcgc
     6421 tgccgcgcat ggaagcgctg cgcgcggtcg cggaccagca tggggtgaac ttcatggcga
     6481 cgatctgcgc gatctgcaag gcgcagttca ccaaggtcct tccgtattac ggcttcaaga
     6541 tgaacatggt cggcggcgtg catcagctcg tcagcacggc gatcaagctc gacgccgcac
     6601 cgcactgaat tccgttggag acatcgatgt ccagcccagc ccagaccgac gcccagccgc
     6661 tgaccttccg ccgcttcaag gacggcgatt tcaagtggaa ggatctgcac cagaagatct
     6721 tcagcgccga tcactcgcac aagtgcccga cctacatcca gtcgacgcca ccgtgccagg
     6781 gcagctgccc gtcgggcgag gacatccgcg ggtatctgaa catcgtgcgc ggcatggaga
     6841 agccgccggc ggggatgagc tggcaggaat acgcgtggag gcgcctgacc gaagccaacc
     6901 cgttcccgtc gatcatgggg cgcgtgtgcc cggcgccgtg cgagaccggc tgcaaccgta
     6961 acgaggtcga ggaccacgtc ggcatcaatt cggtcgagca ctacctcggg gagtgggcga
     7021 tcaagaacga gctgaagttc aacgcgccgg ccaggcgcag cggcaagagc gtggcggtga
     7081 tcggcggcgg tccggcgggc ctctcctgcg cgtaccagct cgcgctgaag ggtcacgacg
     7141 tcacgatctt cgacgagcgc gaatatctcg
//
LOCUS       DBNHBY010000115         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1426838, whole genome shotgun sequence.
ACCESSION   DBNHBY010000115 DBNHBY010000000
VERSION     DBNHBY010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1426838"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="AC16HB_02815"
     CDS             complement(<1..430)
                     /locus_tag="AC16HB_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5605751.1"
                     /translation="MDAVDKHRSTDHKAGSATYTGRCFCGAVQFTVTGEPAAMGYCHC
                     DSCRSWSAAPVNAFTLWKPEAVRITQGADNIGTFNKTPRSFRRWCKTCGGHLLTEHPG
                     WGLTDVYAAVIPEFPYRAGVHVNYQETRLHITDGLPKLKDL"
     gene            599..1579
                     /locus_tag="AC16HB_02820"
     CDS             599..1579
                     /locus_tag="AC16HB_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR5605752.1"
                     /translation="MDALSQVLKSVRLEGAVFLNAEFTAPWCIRGRFGLARARERLRG
                     ADHVVFFHFLVEGRCKVRLVDELGVVAEVLDVTAGDVILLPQDDRYLMGSDLHVAPMD
                     TDNVDAADAAAEPEFIQMHHGGGGSATRFVCGYLGCTRSVIRPLITALPRLLCIPIGD
                     GHAMSLLRDLLRVGVRESLAPRPGAESMLAKLAELIFVEALRRYAEGLPPDVKGWLAA
                     VRDPHVGRALALLHEDPARAWTVDALAREVALSRSALAESFASLVGEPPMQYLTRWRL
                     ALAAQALRTGPEPLARVAARSGYETEAAFNRAFKREFGMPPAAWRRNGVR"
     gene            complement(1602..>2070)
                     /locus_tag="AC16HB_02825"
     CDS             complement(1602..>2070)
                     /locus_tag="AC16HB_02825"
                     /inference="COORDINATES: protein motif:HMM:NF047071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LmrA/YxaF family transcription factor"
                     /protein_id="HIR5605753.1"
                     /translation="RASGAPKGSVYHFFPAGKLQIVSEALALYSERVQAFIEHALSSR
                     RSAAGKVQALFDAFARRVEAGDFLQSCAVGTVSLDLDADLEGLRAVLAAAFDDWVALI
                     ARHVACGDARRSRSFASLVLTTIEGAYIRCRAERSNRPFKEAGVWLADLVARQ"
BASE COUNT          304 a          708 c          725 g          333 t
ORIGIN      
        1 gaagatcctt cagcttcggc aggccgtccg tgatgtgcag ccgggtctcc tggtagttga
       61 cgtgcacgcc ggcgcggtag gggaattcgg gaatcacggc ggcatagacg tcggtgagtc
      121 cccagcccgg atgctcggtg aggagatgtc cgccgcaggt cttgcaccac ctgcggaagc
      181 tgcgcggcgt cttgttgaat gtgccgatgt tgtcggcgcc ctgcgtgatc cgcaccgcct
      241 cgggcttcca cagcgtgaag gcgttgacgg gcgcagccga ccaactgcgg caagaatcgc
      301 agtggcagta gcccatcgcg gccggctcgc cggtgacggt gaactggacc gcgccgcaga
      361 aacagcggcc ggtgtaggtt gcgctgccgg ccttgtggtc ggtggaacga tgtttgtcga
      421 cggcatccat gctgcatttc cctttcggtt gaggtccgcg ccggtcttca tccggcgcga
      481 acggggtcgg tggacacttg cgccggcgcc gatcagtcgg cgcggcgggc gttgctacta
      541 tcgattcgca agcgccttgc gtgttgcccg gaacgccgcg ttccttgtcc gaaaccccat
      601 ggatgccttg tcgcaggttc tcaagtcagt gcggctcgag ggcgccgtct tcctcaacgc
      661 ggaattcacc gcgccgtggt gcatccgcgg ccgcttcggg ctggcccgcg cgcgcgagcg
      721 gctgcgcggc gcggaccacg tggtgttctt tcacttcctc gtggagggac gctgcaaggt
      781 gcggctcgtg gacgaactgg gcgtcgtcgc cgaagtgctc gacgtgaccg ccggcgacgt
      841 gatcctgctg ccgcaggacg atcggtacct gatgggcagc gacctgcacg tcgcgccgat
      901 ggacaccgac aacgtggacg cggcggacgc cgcggccgaa cccgagttca tccagatgca
      961 ccatggcggc ggcggctcgg ccacgcgctt cgtgtgcggc tatctcggct gcacccgcag
     1021 cgtgatccgg ccgctgatca cggcgttgcc gcgcctgctg tgcatcccga tcggcgacgg
     1081 tcacgcgatg tcgctgctgc gcgacctgct gcgggtcggc gtgcgcgaat cgctcgcgcc
     1141 gcggcccggc gccgaatcga tgctcgccaa gctcgccgag ctgatcttcg tcgaagcgct
     1201 gcgccgctat gcggaaggac ttccgcccga cgtcaagggc tggctcgccg cggtgcgcga
     1261 tccgcatgtc ggccgcgcgc tcgccctgtt gcacgaagat ccggcgcggg cctggacggt
     1321 ggacgcactg gcgcgcgaag tcgcgctgtc gcgttcggcg ctggccgaaa gcttcgcctc
     1381 actcgtcggc gagccgccga tgcagtacct gacgcgttgg cgcctggcgc tcgcggcgca
     1441 ggcgctgcgc acgggtccgg aaccgctcgc gcgcgtggcc gcgcgcagcg gctatgaaac
     1501 ggaagccgcc ttcaaccgcg cgttcaagcg cgagttcggc atgccgcccg cagcgtggcg
     1561 caggaacggg gtcaggtgag ccgcggacca ggcgggcccc atcattgccg cgcgaccaga
     1621 tccgccagcc ataccccggc ttccttgaag ggccggttag agcgctcggc ccggcagcgg
     1681 atgtacgcgc cttcgatggt cgtcagcacg aggctggcaa acgacctgct gcggcgcgca
     1741 tcgccgcagg cgacgtgccg cgcgatcaga gcaacccagt catcgaaagc ggccgcgaga
     1801 accgcccgca agccctccag atccgcatcc agatcgagac tcacggtgcc gacggcgcag
     1861 ctctgcagaa agtcgcccgc ctcgacgcgc cgcgcgaagg cgtcgaacaa cgcctgcacc
     1921 ttgccggcgg ccgagcgccg gctcgacaag gcgtgctcga tgaacgcctg cacccgctcc
     1981 gaatagagcg ccagcgcttc gctgacgatc tgcagcttgc cggccggaaa gaagtggtag
     2041 acggagccct tcggcgcccc gctggcgcgc
//
LOCUS       DBNHBY010000116         1685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_479700, whole genome shotgun sequence.
ACCESSION   DBNHBY010000116 DBNHBY010000000
VERSION     DBNHBY010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_479700"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1044
                     /locus_tag="AC16HB_02830"
     CDS             355..1044
                     /locus_tag="AC16HB_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR5605754.1"
                     /translation="MNLDFYEMAIRIVVALAAGALIGYERSYRGRPAGFRTHALVCMA
                     SSMLMLITVYEAHWVRAVGETVRLDPTRMAQGIMTGIGFLGAGVIMKEGLSVRGLTTA
                     ASIWITAAIGVLAGIGFYFPLVLSTVLTLGVLSTFRWIEARMPTQAYYQFEVHFSGDK
                     ATGESDVRNLVEREGLSIANLSFQVDGDRQVRRYSMTIASSDRRAASRLAVALESNGS
                     VQQFRIAPTGD"
     gene            complement(1104..>1685)
                     /locus_tag="AC16HB_02835"
     CDS             complement(1104..>1685)
                     /locus_tag="AC16HB_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5605755.1"
                     /translation="TLVHASFAFVRGEPKKDPLRSLAAKILECEHLVLSGPVPACVVR
                     LGYLYGPESRDLRLYVKAFRLGRPYWSGPRGALQYHLHQHDAVRALLAAARPGNAGRI
                     FYATDGTPVSFMQFMDDFAHRIGRRFPLHVPRIAAPVMRAIVREEHMQQTALAMPARA
                     PRPRVPGWTPEFSDYRSGIDQIIAAWKLAAPGK"
BASE COUNT          290 a          545 c          572 g          278 t
ORIGIN      
        1 ggccagtggg agatcgacat cgccggcacg cgctttccgg cgcgcgcgtc gctcgcgccg
       61 atgtacgacc cgcagatgaa gcggatcaag gcgtagccga cgcatccggc gcgcgcagtc
      121 caagtgcagc gcagggtcgc atgcgactgg aagtcggcgc tcgaagcgac gatagtgaac
      181 gatccgaagc ccgaatctcc gatacccggc gccgcgcgtg gcacggtgat cccgaaagcg
      241 cttcgtcgat ggtgaggacc gcaagggcat cgcgcgaccc gagtcctccg ctgcccgtga
      301 gtaccattct tgcggcaacc gtggacaccg caggccgatc gaggagaacg cgccgtgaat
      361 ctggatttct acgagatggc gattcgcatt gtcgtcgcgc tggcggcggg cgcgctgatc
      421 ggctacgagc gctcgtatcg cggtcggccc gccgggtttc gcactcacgc tctcgtatgc
      481 atggcctcga gcatgctgat gctcatcact gtctatgagg cgcattgggt gcgagcggtg
      541 ggtgaaacgg tccggctcga tccgacccga atggcacagg gcatcatgac cggcatcggt
      601 ttcctcggcg ccggcgtcat catgaaggaa gggctctcgg tccgcgggct cacgacagcg
      661 gcatcgatct ggatcactgc cgccattggc gtgctggccg ggatcggttt ctacttcccc
      721 ctcgtgctat cgacggtgct cacccttggc gtgctctcga cgttccgctg gatcgaggcg
      781 cgcatgccga cgcaggccta ctaccagttc gaggtccact tttccggcga caaagccacg
      841 ggcgagagtg atgtccgcaa cctggtcgag cgcgaggggc tcagcatcgc caacctgagc
      901 ttccaggtcg atggcgatcg gcaggtgcgg cgctacagca tgacgatcgc gtcatcggac
      961 cgacgcgcgg caagtcgtct tgccgttgct ttggaatcaa acggcagcgt gcagcagttc
     1021 cgcatcgcgc caacgggcga ctagaaaaga cagcgccgag cgctcgttcc aggattcgct
     1081 cagcagatat gcaagcgcgc ggttcatttg ccaggcgctg caagcttcca cgccgcgatg
     1141 atctggtcaa tgccgctacg gtaatcggaa aattcgggcg tccaacccgg cacgcgcggc
     1201 cgcggcgcgc gtgcgggcat cgcgagcgcg gtctgctgca tgtgctcctc gcgcacgatc
     1261 gcccgcatca ccggtgccgc aatgcgcggc acgtgcagcg gaaaccgcct gccgatgcgg
     1321 tgcgcgaagt catccatgaa ctgcatgaac gacacgggcg tgccgtcggt ggcgtagaag
     1381 atccttccgg cgttgcccgg acgggcagcc gcgagcagcg cgcgcacggc atcgtgctgg
     1441 tgcaggtggt actgcagcgc gccgcgcggg ccggaccagt acggccggcc gaggcggaac
     1501 gccttcacgt acagccgcag gtcgcggctc tcgggcccgt acaggtagcc caggcgcacc
     1561 acgcacgcgg gtaccgggcc cgacaggacg agatgttcac actcgaggat cttcgcggcg
     1621 agcgagcgca gcgggtcctt tttcggctcg ccgcggacga acgcgaagct ggcgtgcacg
     1681 agcgt
//
LOCUS       DBNHBY010000117         4459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1089138, whole genome shotgun sequence.
ACCESSION   DBNHBY010000117 DBNHBY010000000
VERSION     DBNHBY010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1089138"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="rpsC"
                     /locus_tag="AC16HB_02840"
     CDS             complement(<1..596)
                     /gene="rpsC"
                     /locus_tag="AC16HB_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5605756.1"
                     /translation="MGQKIHPTGFRLAVTRNWNSRWFATGDEFGRMLGEDLRVRDFLR
                     KRLRNASVGRILIERPAKNARITIYSARPGVVIGKKGEDIESLKTALQKMMGVPVHVN
                     IDEIRKPELDAQLISDSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNGI
                     EIARTEWYREGRVPLHTLRADIDYGFSEAKTTYGVIGV"
     gene            complement(606..944)
                     /gene="rplV"
                     /locus_tag="AC16HB_02845"
     CDS             complement(606..944)
                     /gene="rplV"
                     /locus_tag="AC16HB_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR5605757.1"
                     /translation="METTASVRGARLSAQKGRLVADLVRGKPVDKALNILAFTQKKAA
                     GIIRKALESAIANAEHNDGADIDELRVTRICVEQGPTLRRFSARAKGRGTRINKKSCH
                     VYVTVGDGRN"
     gene            complement(954..1247)
                     /gene="rpsS"
                     /locus_tag="AC16HB_02850"
     CDS             complement(954..1247)
                     /gene="rpsS"
                     /locus_tag="AC16HB_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR5605758.1"
                     /translation="MSRSLKKGPFVDGHLLKKVETAVAGKDKKPIKTWSRRSTIVPDF
                     IGLTIAVHNGKQHVPVYVTEQMVGHKLGEFALTRTFKGHSADRKTAPAAPAKK"
     gene            complement(1258..2085)
                     /gene="rplB"
                     /locus_tag="AC16HB_02855"
     CDS             complement(1258..2085)
                     /gene="rplB"
                     /locus_tag="AC16HB_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5605759.1"
                     /translation="MPLVKVKPTSAGRRSLVKVVNPQLHKGRPLASLTEKKIRGSGRN
                     NSGHITMRHQGGGHKQHYRVIDFRRDKDGIAAKVERLEYDPNRSANIALLCYADGERR
                     YVVAPKGLTIGAELQSGQDAPIRVGNTLPLRNIPVGTTVHCVELLPGKGAQLARAAGT
                     SVQLLAREGNYAQLRLRSGEIRKVLIECRATIGEVGNEENNLRQFGKAGVMRWRGIRP
                     TVRGVAMNPIDHPHGGGEGRTGTKRDPVSPWGKLTKGYRTRKNKRTDSMIVQRRYSK"
     gene            complement(2085..2384)
                     /gene="rplW"
                     /locus_tag="AC16HB_02860"
     CDS             complement(2085..2384)
                     /gene="rplW"
                     /locus_tag="AC16HB_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5605760.1"
                     /translation="MSQERLFNVLLGPVVSEKSTMVADKHNQVAFRVLQNATKTEIKA
                     AVEQLFKVRVESVQILNQKGRKKRFGRFSGRRSDVRKAYVSLAPGQEINFAQEVK"
     gene            complement(2387..3007)
                     /gene="rplD"
                     /locus_tag="AC16HB_02865"
     CDS             complement(2387..3007)
                     /gene="rplD"
                     /locus_tag="AC16HB_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5605761.1"
                     /translation="MELKVLNDQGQSTATVAAPATLFGRDYNEALVHQLVVAYQANAR
                     LGTRAQKDRTTVKHSTRKPWRQKGTGRARAGMTSSPIWRGGGKAFPNSPDENFSHKVN
                     KKMYRAGVCSILSKLARDDRIAVIDGLKVDAPKTKLLAQKLKAMGLDSVLIITDALDD
                     NLYLASRNLKNVMVVEPRYADPLSLIHFKKVVLTKPAMVQLEEMWG"
     gene            complement(3018..3683)
                     /gene="rplC"
                     /locus_tag="AC16HB_02870"
     CDS             complement(3018..3683)
                     /gene="rplC"
                     /locus_tag="AC16HB_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR5605762.1"
                     /translation="MSEAAHGQAARLGLVGRKVGMTRIFTDDGETVPVTVIDVSNNRV
                     AQIKSVESDGYSAVQVAYGSKKPSRVSKPVAGQFVKAGVEAAEMLKEFRVDAAKAGDL
                     KAGAALGADLFSVGQKVDVTGISIGKGFAGAIKRHHFSSNRATHGNSVSTNAPGSIGM
                     RQDPGRVMPGKRMAGHLGDVQRTTQNLVVARVDTARQLLLVRGAVPGSRGGNVIVRPA
                     VKG"
     gene            complement(3804..4115)
                     /gene="rpsJ"
                     /locus_tag="AC16HB_02875"
     CDS             complement(3804..4115)
                     /gene="rpsJ"
                     /locus_tag="AC16HB_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR5605763.1"
                     /translation="MQSQRIRIRLKAFDYKLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RIQRFDILRSPHANKTSRDQFEMRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(4242..>4459)
                     /locus_tag="AC16HB_02880"
     CDS             complement(4242..>4459)
                     /locus_tag="AC16HB_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR5605764.1"
                     /translation="FNGYRPQFYFRTTDVTGSIELPSGTEMVMPGDNITMQVALIAPI
                     AMEEGLRFAVREGGRTVGAGVVAKVIE"
BASE COUNT          694 a         1506 c         1359 g          900 t
ORIGIN      
        1 ttcaccccga tcacgccgta ggtggtcttc gcttccgaga agccgtagtc gatgtcggcg
       61 cgcagcgtat gcagcggcac gcgaccctcg cggtaccact cggtgcgcgc gatctcgatg
      121 ccgttcaggc gccccgcgct catgatcttg atgccctggg cgcccagccg catggcgttc
      181 tgcatcgcgc gcttcatcgc gcggcggaac atgatgcgct tttcgagctg ctgcgtgatc
      241 gagtcggaga tgagctgcgc gtcgagctcc ggcttccgaa tctcgtcgat gttcacgtgc
      301 accggcacgc ccatcatttt ctgcagcgcg gtcttcaggg actcgatgtc ctcgcccttc
      361 ttgccgatca ccacgccggg acgggccgaa tagatcgtga tgcgtgcatt cttagccggc
      421 cgctcgatca agatacgccc cacggacgca ttgcgcagac gcttgcggag gaaatcgcgc
      481 acgcgcagat cctcgccaag catgcgaccg aattcgtcgc ccgtggcaaa ccagcgcgaa
      541 ttccagttgc gcgtaacggc caatcgaaag ccggtcggat ggatcttctg ccccatgtgt
      601 actcctcagt tcctgccgtc gccgaccgtc acatacacgt gacacgactt cttgttgatc
      661 cgcgtgccgc ggccctttgc gcgcgccgag aagcgccgca gcgtcggtcc ctgctcgacg
      721 cagatgcgtg tgacgcgcag ctcgtcgatg tccgcgccgt cgttgtgctc ggcgttggcg
      781 atcgccgatt cgagcgcctt gcggatgatt ccggcggcct tcttctgcgt gaaggcgagg
      841 atgttcaacg ccttgtccac cggtttgccg cggaccaggt cggcgaccag ccggcccttc
      901 tgggccgaga ggcgcgcacc gcgtacggag gctgtggttt ccatgctgcc ctcttacttc
      961 ttggccggcg cggcgggcgc cgtcttcctg tcggccgaat gacccttgaa cgtgcgcgtc
     1021 aacgcgaact cgcccagctt gtgaccgacc atctgttccg tgacgtagac cggcacgtgc
     1081 tgcttgccgt tgtgaactgc gatcgtcagg ccgatgaagt ccggcacgat cgtcgagcgg
     1141 cgcgaccacg tcttgatcgg cttcttgtcc ttgcccgcga cggccgtctc gaccttcttg
     1201 agcagatgcc cgtccacgaa cgggcctttc ttcagagaac gcgacattca gtgcccctta
     1261 cttgctgtat cggcgctgca cgatcattga atcggtgcgc ttgttcttgc gcgtgcgata
     1321 gcccttggtg agcttgcccc aagggctgac cgggtcgcgc ttggtgccgg tgcgaccctc
     1381 gccgccgccg tgcgggtgat caatcgggtt cattgccacg ccgcgcacgg tcgggcgaat
     1441 gccccgccaa cgcatgactc cggccttgcc gaactggcgc aggttgttct cctcgttgcc
     1501 gacctcgccg atcgtcgcgc ggcactcgat cagcaccttc cggatctcgc ccgaacgcag
     1561 gcgcagctgc gcgtagttgc cctcgcgcgc cagcagctgc accgaggtgc cggcggcacg
     1621 cgccagctgc gcgcctttgc ccggcagcag ctcgacgcag tgaaccgtcg tgccgaccgg
     1681 aatgttccgc agcggcagcg tgttgccgac gcgaatcggt gcgtcctgcc cgctctgcag
     1741 ctcggcgccg atcgtcaggc ccttgggcgc cactacgtaa cggcgctcgc cgtccgcata
     1801 gcacagcagc gcgatgttcg ccgagcggtt tgggtcgtac tccaatcgct cgaccttggc
     1861 ggcaatgccg tccttgtcgc gccggaaatc gatgacacgg tagtgctgct tgtgaccacc
     1921 gccttggtgc cgcatcgtga tgtggccgct gttgttgcgg cccgatccgc ggatcttctt
     1981 ctcggtcagc gacgcaagcg gcctgccctt gtgcagctgc gggttgacga ccttgaccag
     2041 cgaccggcgg ccggccgatg tcggtttgac tttgaccagc ggcattactt cacctcttgc
     2101 gcgaagttga tttcctgtcc gggcgcgagc gaaacgtaag ccttgcgcac gtcgctgcgc
     2161 cgcccgctga agcgcccgaa gcgcttcttc ctgcccttct ggttcaggat ctggaccgac
     2221 tcgacccgca ccttgaacaa ctgctcgacg gcagccttga tttcggtctt ggttgcgttt
     2281 tgcagcacgc ggaaggcgac ctggttgtgc ttgtcagcaa ccatcgtgct cttctcggag
     2341 acaacaggtc cgagcagtac gttgaacaaa cgctcctggc tcatcgtcat ccccacattt
     2401 cctcgagctg gaccatcgcc ggcttggtca gtactacttt cttgaagtgg atcagcgaca
     2461 gtggatcggc atagcgcggc tccaccacca tcacgttctt caggttgcgc gaagcgaggt
     2521 acaggttgtc atcgagcgca tcggtgatga tcagcaccga atcgagcccc atcgccttca
     2581 gcttctgcgc cagcagcttg gtcttgggcg catcgacctt caggccgtcg atcaccgcga
     2641 tgcggtcatc ccgcgccagc ttcgacagga tcgaacacac gccggcccgg tacatcttct
     2701 tgttcacctt gtgcgagaag ttctcgtcgg gcgagttcgg aaaggccttg ccgccgcccc
     2761 gccagatcgg gctggaggtc atcccggcac gcgcgcgtcc ggtacccttc tgccgccacg
     2821 gcttgcgcgt cgaatgcttg accgtcgtac ggtccttttg ggcgcgggtg cccagacgcg
     2881 catttgcctg gtacgccacc acgagctgat gtaccagcgc ttcgttgtag tcgcgaccga
     2941 acagcgtggc gggtgcagcc accgtggcgg tcgactggcc ctggtcgttc aagactttga
     3001 gttccatcga acgctcctta gcctttcacc gccggacgca cgatcacatt gccgcccctg
     3061 gatccaggca ccgcgccgcg aaccagcagg agctgacgcg ccgtgtccac acgcgcgacc
     3121 acgaggttct gcgtcgtccg ctgcacgtcg cccaagtgac cggccatgcg cttgcccggc
     3181 atcacccgac ccgggtcctg ccgcatgcca atcgatccgg gcgcgttggt cgacaccgag
     3241 ttgccgtgcg tggcgcggtt cgacgagaag tgatggcgct tgatcgcgcc agcaaagccc
     3301 ttgccgatcg aaatgcccgt cacgtcgact ttctggccga cactgaaaag atcggcaccg
     3361 agcgcggcac cggccttcag atcgcccgcc ttggcggcgt caacgcggaa ttccttcagc
     3421 atctcggccg cttcgacgcc ggccttgacg aactggcccg cgactggctt gcttacccgc
     3481 gagggcttct tgctgccgta ggcgacctgg accgcggaat agccgtcgga ctcgacggac
     3541 ttgatctgcg cgacgcggtt gttggaaaca tcgatgacgg tgaccggaac ggtctcgccg
     3601 tcgtcagtga agatgcgcgt catgccgacc ttgcggccga ccaaacccag acgcgcagcc
     3661 tgaccgtgcg ctgcttcgct catcgttttt ctccacaccc cgactgcaat tggccgggac
     3721 tgattccggt cgtctcctgg atcgatgatc cggaaacgga aagttcgtga ttctactgaa
     3781 tacgggccgc tcgccgcaac ccgctactgc accttgatct cgacgtcgac gcccgccggc
     3841 aggtcgagct tcatcagcgc gtcgaccgtc ttgtcggtcg ggtcgatgat atccatcagc
     3901 cgcaggtgcg tgcgcatctc gaactggtcg cgcgaggtct tgttggcatg cggcgagcgc
     3961 aggatgtcga agcgctggat gcgcgtcggc aggggcaccg ggccgcgcac gaccgcgccg
     4021 gtccgtttcg ccgtatcgac gatttccagc gccgactggt cgatcagctt gtagtcgaac
     4081 gccttcaggc ggatccggat tcgttggctt tgcatgtctg ttccctcaaa gagcaagcgg
     4141 cgctgcgcgc cgcagaaagc aggtcggcgg tcctcaatcg gcagtcgatt ccaccgtcgg
     4201 cgatcgcggg cgccgccgac ggtcgacagc cgacttgccg actactcgat cacctttgcc
     4261 acgacgccgg cgccgacggt gcggccgccc tcgcgcaccg cgaagcgcag gccctcctcc
     4321 atcgcgatgg gtgcgatcag cgccacctgc atcgtgatgt tgtcccccgg catcaccatc
     4381 tcggtgccgc tgggcagttc gatggaaccc gtcacatccg tggtgcggaa gtaaaactgc
     4441 ggccgataac cgttgaaaa
//
LOCUS       DBNHBY010000118         5808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1292138, whole genome shotgun sequence.
ACCESSION   DBNHBY010000118 DBNHBY010000000
VERSION     DBNHBY010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1292138"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="AC16HB_02885"
     CDS             complement(<1..1051)
                     /locus_tag="AC16HB_02885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5605765.1"
                     /translation="MIEFRLPSLGADMDEGTLLEWRVQPGGTVKRGQIVCVVDTAKAA
                     IDVECWQEGIVHALLIEPGTKIPVGTLMAALRAPDESVEAVERALAVLKAKETGPRPL
                     APTPVAAETRLVTTVPPPTRLRASPAAKRRAEELHIDLAQVSGSGPDGAITILDVEAA
                     ARAAPPPAPKPAPAATRAEEMRRVIAAAMARSKREIPHYYLSEDIPLAAATAWLAQEN
                     AQRPVTNRLLLAPLLLKAVAAALRRYPELNGYFREGAFEASAAIHIGVAISLRAGGLV
                     TPALHDVGRKDMDTVNRELLDLTQRTRAGSLRLTELTDATVTVTSLGDRGVGAVFGVI
                     FPPQVAIVGFGRVTDR"
     gene            complement(1048..1521)
                     /locus_tag="AC16HB_02890"
     CDS             complement(1048..1521)
                     /locus_tag="AC16HB_02890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5605766.1"
                     /translation="MDKATERFIEKELNRHRILTLATVRPDGFPQATIVTFAHDGLTL
                     FIAVDAKSQKARNIRRNNKVSLAIGRDQRDWGRITGLSMAAQARILRRRDEIAWAKAL
                     LGKRFAQMKEMGEADDYAGWAFIAIVPLVISVLDYTQGLGHTELVKLWPRQVQRR"
     gene            complement(1531..2511)
                     /locus_tag="AC16HB_02895"
     CDS             complement(1531..2511)
                     /locus_tag="AC16HB_02895"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5605767.1"
                     /translation="MTHTTYREALRAAHHEALTRDARVFIMGEDVCRYGGSYAVTKGF
                     YDEFGPERIRDTPLSELAFVGAGIGAALGGLRPIVEVMTVNFSLLALDQIVNSAATIR
                     HMSGGQFSIPLVIRMATGAGRQLAAQHSHSLEVWYAHIPGIRVLAPATLEDARGMLWP
                     ALQDPDPVVLFEHAQLYNLEGELADPPPQVDIARATVRRAGTNLSIITYGGSLPRALA
                     AADELARDGIDAEVVDLRVLRPLDWDTLVASVRRTRRALVVDEGWKTCSLAAEIMAGL
                     VERAFYDLDAPLARVCTEEVPIPYAKHLEDAALPLPPRIVAAARSLVAER"
     gene            complement(2508..3068)
                     /locus_tag="AC16HB_02900"
     CDS             complement(2508..3068)
                     /locus_tag="AC16HB_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR5605768.1"
                     /translation="MKRILVVYGTTEGHTRKIAEFVAERLRKAGKTVDLVDSATPAAA
                     QIAPIYAGAFIGGSLHQGRHQAPLAHFIKVNAAWLNTIPTAFFSVSLSMASQDAEWQA
                     EAKRLADAFLADTGLNAGMVRLVAGALMYTEYDFFRRWMMKAIAKKAGGSTDTSRDAD
                     YTDWNDVARFVDEYLAATRGAERMPA"
     gene            complement(3065..4090)
                     /gene="pdhA"
                     /locus_tag="AC16HB_02905"
     CDS             complement(3065..4090)
                     /gene="pdhA"
                     /locus_tag="AC16HB_02905"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR5605769.1"
                     /translation="MTPEELTRFTTDVTKYPREFCLARVRDMVRIRRLEERCAELYGA
                     GRIRGFLHLYIGEEAVAAGVLPNLTRDDAVVATYREHGQALLRGVPMNAIMAEMYGKA
                     EGCSHGRGGSMHLFDAATRFYGGNAIVGGGLPLAVGLALADRMAGRKGRVTACFFGEG
                     AVAEGVFHESLNLAALWRLPVLFLCENNLYAMGTALARSESQIDLTKKAASYGIATRH
                     INGMNVLAVHEAAQAAADAVRADGLPYFVEFHTYRFRAHSMFDAELYRNKDEVERWKQ
                     HGPIHTFTDRLKAAGMMTEDDFAAIDRAAQAEVDAAVAFAEAGTWEPAEQLTRDVTTP
                     AQQRSNA"
     gene            complement(4101..4769)
                     /locus_tag="AC16HB_02910"
     CDS             complement(4101..4769)
                     /locus_tag="AC16HB_02910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5605770.1"
                     /translation="MTKTLKERIARARRIAAKALLLAALLVIAEVAGQEMVLPPVQTD
                     GGKPLMQALKDRRSTRSFAEQPLPTQVLSNLLWAAFGVNRPDGHRTAPSARNWQEIDV
                     YVALREGLYVYDARAHALKLTVAKDLRATAGTQAHAREAPVTLVYVADERRMGRTDDE
                     TRRTFAASDAGFIAQNVYLYCASEGLATVVFASIDRERFAQAAGLAPAQKITLAQSVG
                     YPKR"
     gene            complement(4786..5307)
                     /locus_tag="AC16HB_02915"
     CDS             complement(4786..5307)
                     /locus_tag="AC16HB_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR5605771.1"
                     /translation="MRRTLIVYHSRTGNTRRVAQTLAQRLDADLEEIRIVQPMTGALG
                     YAFCALEAIARLAPALRPLHKDPTAYDLVVIGTPVWFWSLSSPVRSWLERHPLKQRVA
                     FFCTMGGSGARRVFAAMAGLAGREPVATLALTDREVAAGSNKLDAFVGELHAGAPRRP
                     SRAPRARAAHATG"
     gene            complement(5323..>5808)
                     /locus_tag="AC16HB_02920"
     CDS             complement(5323..>5808)
                     /locus_tag="AC16HB_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR5605772.1"
                     /translation="LNNEERYRKCFVESSQGRLYLTEDMVKRDAEGYFWFVGRADDVI
                     KSAGHLIGPFEVESALMEHPAVAEAGVIGKPDPVVGEAVKAFVSLKPGFEASEALRRE
                     LLGHARKRLGPAVAPKEIDFTAALPRTRSGKIMRRLLKARELGLPEGDTSTLEAGAPS
                     N"
BASE COUNT          791 a         2086 c         1982 g          949 t
ORIGIN      
        1 gccggtcggt gacgcgtccg aacccgacga ttgccacttg cggcggaaag atcacgccga
       61 acaccgcgcc gacgccgcgg tcgccgaggc tggtcaccgt gaccgtcgcg tcggtcagtt
      121 ccgtcagccg cagcgagccg gcgcgcgtgc gctgtgtcag gtcgagcagt tcgcggttga
      181 cggtgtccat gtccttgcgg ccgacgtcgt gcaacgccgg ggtgaccagc ccgcccgcgc
      241 gcagcgagat tgcgacaccg atgtggatcg ccgccgacgc ctcgaacgcg ccctcgcgga
      301 aatagccgtt caattccgga taccggcgca gcgccgccgc cacggccttc agcagcagcg
      361 gcgccagcag caaccggttc gtcaccggtc gctgcgcgtt ctcctgcgcg agccaggcgg
      421 tggccgccgc tagcggaatg tcctcggaca ggtagtagtg cgggatctcg cgcttggaac
      481 gcgccatcgc ggcggcgatc acgcgccgca tctcctcggc gcgcgtggcg gccggcgcag
      541 gtttcggggc cggcggcggc gcggcgcgcg cggcggcttc gacgtcgagg atcgtgatcg
      601 ctccatcggg cccgctgccg ctcacctgcg cgaggtcgat gtgcagttcc tccgcgcgcc
      661 gcttggcggc cggcgatgcg cgcagccgcg tcggcggtgg cacggtcgtg acgaggcgag
      721 tttcggcggc cacgggcgtc ggcgccagcg gcctcggtcc cgtttccttc gccttcagca
      781 cggcgagcgc gcgttccacg gcctccacgc tctcgtccgg cgcgcgcaac gccgccatca
      841 gcgtgccgac cgggatcttc gtgccgggct cgatcagcag cgcgtgcacg atcccttcct
      901 gccagcactc gacgtcgatc gccgccttcg cggtgtcgac cacgcagacg atctggccgc
      961 gcttgaccgt gccgccgggc tgcacgcgcc actcgagcag cgtgccttcg tccatgtcgg
     1021 cgccgagcga gggcaggcgg aactcgatca tcggcgctgc acctgcctgg gccacagctt
     1081 caccagttcc gtgtggccga ggccctgcgt gtaatcgagc accgagatca ccagcggcac
     1141 gatcgcgatg aacgcccagc cggcgtagtc gtccgcttcg cccatctcct tcatctgcgc
     1201 gaagcgcttg ccgagcagcg ccttcgccca ggcgatctca tcgcgccggc gcaggatgcg
     1261 cgcctgtgcg gccatcgaga gtccggtgat gcggccccag tcgcgctggt cgcggccgat
     1321 cgcgagcgac accttgttgt tgcggcggat gttgcgcgcc ttctgactct tcgcgtccac
     1381 cgcgatgaac agcgtcaacc cgtcgtgcgc gaaggtgacg atcgtcgcct gcggaaatcc
     1441 gtccggccgc accgtggcga gcgtcagtat ccggtggcgg ttcagctctt tctcgatgaa
     1501 gcgctcggtc gccttgtcca ttgtcggctc ctaccgctct gcgacgagcg atctcgccgc
     1561 ggcgacgatc cgcggcggca gcggcagcgc ggcgtcttcg aggtgcttcg catacgggat
     1621 cggcacctcc tcggtgcaca cgcgcgccag cggcgcatcg aggtcgtaga acgcgcgctc
     1681 gaccagcccc gccatgatct cggccgcgag gctgcaggtc ttccagcctt cgtcgaccac
     1741 cagcgcgcgg cgcgtccggc gcacggacgc gaccagcgtg tcccagtcga gcgggcgcag
     1801 cacgcgcagg tcgaccactt cggcgtcgat gccgtcgcgt gccagttcgt ccgccgcggc
     1861 gagcgcgcgc ggcaggctgc cgccataggt gatgatcgag agattcgttc ccgcgcggcg
     1921 caccgtggcg cgcgcgatgt cgacctgcgg cggcggatcg gcgagttcgc cttcgaggtt
     1981 gtacagctgc gcgtgctcga acagcaccac cgggtccggg tcctgcagcg ccggccacag
     2041 catgccgcgc gcatcctcga gcgttgccgg cgccagcacc cggatgccgg ggatgtgcgc
     2101 gtaccagacc tcgaggctgt gcgaatgctg cgcggccagc tgccgccccg cgccggtcgc
     2161 catgcggatg acgagcggga tcgagaactg cccgcccgac atgtggcgga tggtcgccgc
     2221 gctgttgacg atctggtcga gtgcgagcag cgagaagttc accgtcatca cctcgacgat
     2281 cggccgcagg ccgcccagcg ccgcgccgat gccggcgccg acgaatgcga gttccgacag
     2341 cggcgtgtcg cggatgcgct cggggccgaa ttcgtcgtag aagcccttgg tcaccgcgta
     2401 cgaaccgccg tagcgacaga cgtcctcgcc catgatgaag acgcgcgcgt cgcgggtcag
     2461 cgcctcgtgg tgggcggcgc gcagtgcttc gcggtaggtg gtgtgcgtca tgcgggcatg
     2521 cgctcggcgc cgcgcgtggc ggccaggtac tcgtcgacga agcgcgcgac gtcgttccag
     2581 tccgtgtagt cggcgtcacg cgacgtgtcg gtgctgccgc cggctttctt cgcgatcgcc
     2641 ttcatcatcc agcggcggaa gaagtcgtac tcggtgtaca tcagcgcgcc ggcgacgagc
     2701 cgcaccatgc cggcgttcaa ccccgtgtcg gcgaggaacg cgtcggcgag ccgcttcgct
     2761 tccgcttgcc actcggcgtc ctggctcgcc atcgacaggc tgaccgagaa gaacgcggtc
     2821 gggatcgtgt tcagccacgc ggcattgacc ttgatgaagt gcgcgagcgg cgcctggtgc
     2881 ctgccctggt gcagcgagcc gccgatgaac gcgccggcat agatcggcgc gatctgcgcc
     2941 gcggccggcg tggcgctgtc gaccagatcg accgtcttgc ccgccttgcg caatcgttcg
     3001 gcgacgaact ccgcgatctt gcgcgtgtgt ccttcggtgg tgccgtagac gacgaggatc
     3061 cgtttcatgc gttgctcctc tgttgcgcgg gcgtggtcac gtcgcgcgtg agctgctcgg
     3121 ccggctccca cgtgcccgct tcggcgaatg ccacggctgc atcgacctcg gcctgcgcgg
     3181 cgcggtcgat cgccgcgaag tcgtcttcgg tcatcatgcc ggccgccttc agccggtcgg
     3241 tgaaggtgtg gatcgggcca tgctgcttcc agcgttcgac ctcgtccttg ttgcgataca
     3301 gctctgcgtc gaacatcgag tgcgcgcgga agcggtaggt gtggaattcg acgaagtacg
     3361 gcagcccatc ggcacgcacc gcatcggccg ctgcctgggc cgcttcgtgc acggcgagca
     3421 cgttcatgcc gttgatgtgg cgggtcgcga tgccgtagct cgcagccttc ttcgtcaggt
     3481 cgatctgcga ctcggagcgc gcgagcgccg tacccatcgc gtacaggttg ttctcgcaca
     3541 ggaacagcac cggcagccgc cacagcgcgg cgaggttcag cgattcgtgg aacacgcctt
     3601 cggccaccgc gccttcgccg aagaagcacg cggtcacgcg ccccttgcgc ccggccatcc
     3661 tgtccgccag cgccagcccc accgccagcg gcaggccgcc gccgacgatc gcgttgccgc
     3721 catagaagcg ggtcgcggcg tcgaacagat gcatcgaacc gccgcgcccg tgcgagcagc
     3781 cttccgcctt gccgtacatc tcggccatga tcgcgttcat cggcacgccg cgcagcagcg
     3841 cctggccgtg ctcgcgatag gtcgccacca ccgcatcgtc gcgcgtcagg ttcggcagca
     3901 ctccggcggc gaccgcttcc tcgccgatgt acaggtgcag gaagccgcgg atcctgcctg
     3961 cgccgtacaa ctcggcgcag cgctcctcga gccggcggat gcgcaccatg tcgcgcacgc
     4021 gcgcgaggca gaactcgcgc gggtacttcg tcacgtcggt ggtgaagcgc gtcagttctt
     4081 cgggcgtcat tgccgcatcc tcagcgtttc ggatacccca ccgactgcgc cagcgtgatc
     4141 ttctgcgcgg gcgcaagccc cgctgcctgc gcgaagcgct ctctgtcgat cgacgcgaac
     4201 acgaccgtcg ccagtccttc cgaggcgcag tacagataga cgttctgcgc gatgaagccg
     4261 gcatcggagg cggcaaaggt ccgtcgcgtt tcgtcgtcgg tccgtcccat gcggcgctcg
     4321 tcggcgacat agaccagcgt gaccggcgcc tcgcgcgcat gcgcctgcgt tcccgcggtc
     4381 gcgcgcaggt ccttggccac cgtgagcttc agcgcgtgcg cccgcgcgtc gtacacgtac
     4441 agtccctcgc gcagcgcgac gtagacatcg atctcctgcc agttgcgcgc cgacggcgcc
     4501 gtgcgatggc cgtcggggcg attgacgccg aacgccgccc acagcaggtt cgacagcacc
     4561 tgcgtcggca acggctgctc ggcgaagctg cgcgtggagc gccggtcctt cagcgcctgc
     4621 atcagcggtt tgccgccgtc cgtctgcacg ggcggcagca ccatctcctg gccggccacc
     4681 tcggcgatca ccagcagcgc ggcgagcagc agcgccttgg cagcgatgcg gcgcgcgcgg
     4741 gcgatgcgtt ccttcagtgt cttcgtcatc gcggcctccg tgcgctcagc cggtcgcgtg
     4801 cgccgcgcgc gcgcgtggtg cgcggctcgg ccggcgcggc gcgcccgcgt gcaactcccc
     4861 cacgaaggcg tcgagcttgt tgctgccggc agcgacttcc cgatcggtca gcgccagcgt
     4921 ggcgaccggc tcgcgaccgg cgagtccggc catcgccgcg aacacgcgcc gcgctcccga
     4981 gccgcccatc gtgcagaaga acgcgacgcg ctgcttgagc ggatgccgct cgagccaact
     5041 gcgcaccggg ctcgacaggc tccagaacca caccggcgtg ccgatcacga ccaggtcgta
     5101 cgcggtcggg tccttgtgca gcggtcgcag cgcgggcgcg agccgcgcga tcgcctcgag
     5161 cgcgcagaac gcgtagccga gcgcgccggt catcggctgc acgatgcgga tctcttcgag
     5221 gtccgcgtcg agccgctgcg ccagcgtctg tgccacgcgg cgggtgttgc ccgtgcgcga
     5281 gtgatagacg atcagggtcc tgcgcatgat gtcctccggg actcagttcg atggcgctcc
     5341 cgcctccagc gtcgacgtat cgccttcggg caatccgagt tcgcgcgcct tcaggagccg
     5401 gcgcatgatc ttgccgctgc gcgtgcgcgg cagagcggcc gtgaagtcga tttccttcgg
     5461 cgcgaccgcg ggaccgaggc gcttccgcgc atggccgagc agttcgcgcc gcagcgcctc
     5521 gctcgcctcg aagccgggct tcagcgacac gaacgccttg accgcctcgc ccaccaccgg
     5581 gtcgggcttg ccgatcacgc cggcctcggc gacggcgggg tgttccatca gtgcgctctc
     5641 gacctcgaac gggccgatca ggtggcccgc gctcttgatc acgtcatcgg cgcggccgac
     5701 gaaccagaag tacccttcgg cgtcgcgctt gaccatgtcc tcggtcaggt acagccgacc
     5761 ttggctcgac tcgacgaagc acttgcggta gcgctcctcg ttgttcag
//
LOCUS       DBNHBY010000119         3151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1765677, whole genome shotgun sequence.
ACCESSION   DBNHBY010000119 DBNHBY010000000
VERSION     DBNHBY010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1765677"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16HB_02925"
     CDS             complement(<1..200)
                     /locus_tag="AC16HB_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR5605773.1"
                     /translation="MTTRLAAAVAAAIACAGCSSMYAAGPSAGPSATAELKPTQGNNA
                     TGTVQFMQQGNKVAVMVSVRGLP"
     gene            complement(279..500)
                     /locus_tag="AC16HB_02930"
     CDS             complement(279..500)
                     /locus_tag="AC16HB_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605774.1"
                     /translation="MAWLDHIPLWLIVAVAIWLAVAPLVPEPHLTEKLGMLVHGTLKR
                     PLDVFDLLYHLAPIVVLVLKLARMRQEKS"
     gene            complement(506..2167)
                     /gene="maeA"
                     /locus_tag="AC16HB_02935"
     CDS             complement(506..2167)
                     /gene="maeA"
                     /locus_tag="AC16HB_02935"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HIR5605775.1"
                     /translation="MAANPSPAAQARRELPHNAALLRDPALNKGTAFTEAERDALGLH
                     GLLPPHVATPEEQEARVMENLHRLQVPLNKYVALEALHDRNETLFYRVVINHIDEMMP
                     VIYTPTVGLACQQFGHIYRRPRGMYVSAADRGRVARVLANWPTREVDIIVVTDGERIL
                     GLGDLGANGMGIPVGKLALYTACAGIHPQRCLPIVLDVGTNTDAIRSDPLYLGLRRRR
                     LAGAEYDALIDEFVLAAQQVFPGVLVQFEDFANHHAFHLLERWRRRVPCFNDDIQGTA
                     AVTVAGLLSALRVTGNRLDEHRFLFLGAGEAATGIADLITAAMVDGGTTVEAARGRSW
                     LFDSKGLVVAERTDLAAHKEPYAHAHAPVADFVAAIERLKPTAIIGVAATGGAFTEAV
                     LRTMARLNARPIVFALSNPTSKGECTAEQAYRSTDGRALFASGSPFDPVTQDGRTFVP
                     RQGNNSYVFPGIGLGAIVSRAREVTDEMFLAAARSLATQVSESDLAQGSLYPPLSAVR
                     EVSAHIAAAVAEVAYARGLAGKPRPADVLADVRAAMYEPVYASYA"
     gene            complement(2303..2956)
                     /locus_tag="AC16HB_02940"
     CDS             complement(2303..2956)
                     /locus_tag="AC16HB_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR5605776.1"
                     /translation="MHPLPGLSLLETRILGVLVEKEKTVPDTYPLSLNALVGGCNQKN
                     NRDPVLSATETEVQAAIDRLKSLSLVIESSGARVARYSHNAGRVLGVPAQSVALLATL
                     MLRGAQTAGELRIASERLHRFADISSVEAFLDELATRPDSNGGALVVKLPRQPGEREQ
                     RWAHLLSGAVAAPAQAEATAAADASPDIAMLRAEVARLADEVAQLRALVTRLQSSEP"
BASE COUNT          472 a         1087 c         1088 g          504 t
ORIGIN      
        1 ggcaatcccc gcacgctgac catgacggct accttgttgc cctgctgcat gaactgcacg
       61 gtgcccgtcg cgttgttgcc ctgggtcggc ttcagctccg ctgtggccga ggggcccgct
      121 gacgggccgg cggcgtacat cgaggaacag ccggcgcatg cgatcgcggc cgcgactgcg
      181 gcggcaagtc tggtcgtcat cttcgatctc ccgcggtgtc gtcggtgcca gggcatgcac
      241 gctagcaccg ggcgcgactt cccgccacac ggccgcgatc aggatttctc ctgccgcatc
      301 cgcgcgagct tcagcaccag caccacgatc ggcgcgaggt ggtagagcag gtcgaagaca
      361 tcgagcggcc gcttcagcgt gccgtgcacg agcatcccga gcttctctgt caggtgcggc
      421 tccggcacga gtggcgcgac ggcgagccag atcgccaccg cgacgatgag ccacagcggg
      481 atgtggtcca gccacgccat ccgcactacg cgtagctcgc gtacaccggc tcgtacatcg
      541 ccgcgcgcac atcggccagc acgtcggcgg ggcgcggctt gccggcgagc ccacgcgcgt
      601 acgcgacctc tgccaccgcg gcagcaatgt gcgccgacac ctcacgcacc gcgctgagcg
      661 gcggatacag gctgccctgc gcgagatccg actcgctgac ctgcgtggcg agcgagcgcg
      721 cggcggcgag aaacatctcg tcggtcactt cgcgcgcgcg gctgacgatc gcgcccagcc
      781 cgatgcccgg gaacacatac gagttgttgc cctgccgcgg cacgaaagtg cggccgtcct
      841 gcgtgaccgg atcgaacggg ctgccggagg cgaacagcgc gcgcccgtcc gtggagcggt
      901 acgcctgctc ggcagtgcat tcgcccttcg aggtcgggtt cgacagcgcg aacacgatcg
      961 gccgcgcatt cagccgcgcc atcgtacgca gcaccgcctc ggtgaacgcg ccgccggtgg
     1021 cagccacgcc gatgatcgcg gtcggtttca agcgttcgat cgcggcgacg aagtcggcga
     1081 ccggggcgtg cgcatgcgcg tacggctcct tgtgcgcggc cagatccgtg cgttccgcga
     1141 cgaccagccc cttgctgtcg aagagccagc ttcttccgcg cgccgcttcc accgtcgtgc
     1201 cgccgtcgac catcgcggcg gtgatcagat cggcgatgcc ggtggcggcc tcgcccgcac
     1261 cgaggaagag gaaccggtgc tcgtcgagcc ggttgcccgt cacgcgcagc gccgacagca
     1321 ggccggcgac ggtgaccgcc gcggtgccct ggatgtcgtc gttgaagcac ggcacgcgac
     1381 ggcgccagcg ttcgagcaga tggaacgcgt ggtgattggc gaaatcctcg aactggacca
     1441 gcacgccggg gaacacctgc tgcgccgcga gcacgaactc gtcgatcagc gcgtcgtact
     1501 cggcgccggc gaggcgccgg cgccgcaacc ccagatacag cggatcgctg cggatcgcgt
     1561 cggtgttggt gccgacgtcg agcacgatcg gcaggcagcg ctgtggatgg atgccggcgc
     1621 acgcggtgta cagcgccagc ttgccgaccg gaattcccat gccgttggcg ccgaggtcgc
     1681 ccagccccag gatgcgctcg ccatcggtga cgacgatgat gtcgacttcg cgcgtgggcc
     1741 agttggcgag cacccgtgcc acgcggccgc ggtcggcggc gctgacgtac atgccgcgcg
     1801 ggcgccggta gatgtggccg aactgctggc acgccagacc cacggtcggg gtgtagatga
     1861 ccggcatcat ctcgtcgatg tggttgatca ccacgcgata gaacagcgtc tcgttgcggt
     1921 cgtgcagcgc ctccagcgcg acgtacttgt tcagcggcac ctgcagccgg tgcaggttct
     1981 ccatcacgcg cgcctcctgc tcttccggcg tggccacgtg cggcggcagc aggccgtgca
     2041 ggccgagcgc atcgcgctcg gcttcggtga aggcagtgcc cttgttcagg gccgggtcgc
     2101 gcagcagcgc ggcgttgtgc ggcagttcgc ggcgcgcctg cgccgccggc gaaggattcg
     2161 cagccatcgt ggttctccag tggatgcggc cagcgcgacg cgctggcgcg ttaatggtct
     2221 ggcgccactc taggacgcaa gcgccggacc gtgttgacgg tgcgcaagcg cgtcggcacg
     2281 gagtcggtca ggtactccgc gatcagggtt ccgacgattg cagccgcgtg acgagcgccc
     2341 gcagctgcgc gacttcgtcc gccagccgtg ccacttccgc gcgcagcatt gcgatgtcgg
     2401 gcgaagcgtc cgccgccgcc gtggcctcgg cttgcgccgg cgccgcgacg gctccggaca
     2461 aaaggtgcgc ccagcgctgc tcccgctcgc cgggctggcg cggcagcttg acgaccagcg
     2521 cgccgccgtt cgaatcgggc cgcgtagcga gttcgtccag gaacgcctcg accgacgaga
     2581 tgtcggcgaa ccgatgcagg cgttcgctcg cgatgcgcag ttcgccggcg gtctgcgcgc
     2641 cgcgcagcat cagcgtcgcg agcagcgcga ccgactgcgc cggaacgccg agcacgcggc
     2701 cggcgttgtg cgaatagcgc gccacgcgcg ccccgctcga ctcgatgacg agcgacagcg
     2761 acttcagccg atcgatcgcg gcctgcacct cggtttcggt cgcgctgagc accgggtcgc
     2821 ggttgttctt ctggttgcag cctccgacca gcgcattcag cgacaacgga tacgtgtcgg
     2881 gaacggtctt ctccttctcg accagcacgc cgaggatgcg tgtttcgagc agtgagagtc
     2941 cgggaagcgg atgcatggct tgtatgcccg aatcagacgc ggcaatatgc cttggcgccg
     3001 cagagcacga attcgaccgt gtgtccttgt atgttcttct ttagctgatt gtttgcagac
     3061 ccactcggcg ctgctgcatc gttgtgctcc cttctgcggg cactccatgc agaagtagga
     3121 ccgcgaggca agctgcgctg tcctctacgg c
//
LOCUS       DBNHBY010000120         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_344850, whole genome shotgun sequence.
ACCESSION   DBNHBY010000120 DBNHBY010000000
VERSION     DBNHBY010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_344850"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="AC16HB_02945"
     CDS             <1..1580
                     /locus_tag="AC16HB_02945"
                     /inference="COORDINATES: protein motif:HMM:NF027637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5605777.1"
                     /translation="DADWDTLEGRHMAVKWFVDSSLAGTAAIGPHQSDPRTGEILYGA
                     VLIPDVWARISGQRFGEVLPPRVQLPTGLSNLLRSEQCSYAFDALEQAAFSFELLVER
                     GAIARDSEAARRFILDSIKEVVMHEVGHALGLRHNFKGSSAIKFDQLRDGGFTEKYGL
                     SSSIMDYVPENIPLDGEARTGRLQMDTIGTYDRWAIEWGYRQFAAADEAKLLSALAAR
                     SATDPLLAYATDDDAGSDGDIGSGPPGLDPRANRFDLGEDSLVYYERQLKLTRELFAR
                     TQRRKLDADDDYRLYRRNLERGLRQMRGVAPNVAKYVGGLYVNRDRAASGRPLLTPVE
                     APRQRAALKLLTAELFSTASFAFDPQFMQRLGVDQFARGNTGPDFSLPSAVLDIQRPV
                     LDQLMSEMVALRMANAESKSAEPRALLDFAEVQSTLTDAVWSELKTGQDIGTLRRNLQ
                     REHLRRLASALVRPISTVAADVRSVQRQQAERLVAQLKRALTSKGKRSDVAQAHLAES
                     LVTLREALAAPMYRTGV"
     gene            complement(1588..2223)
                     /gene="ureG"
                     /locus_tag="AC16HB_02950"
     CDS             complement(1588..2223)
                     /gene="ureG"
                     /locus_tag="AC16HB_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIR5605778.1"
                     /translation="MRVGPPNHPPLRVGVGGPVGSGKTTLVEKLCKQMRSQYQLAVVT
                     NDIYTKEDERLLVDAQALAPERIMGVETGGCPHTAIREDASINLEAIDRMCQRFPDLD
                     LIFIESGGDNLAATFSPELSDLTIYVIDVAGGEKIPRKGGPGITKSDLLVINKTDLAP
                     HVGARLDVMRHDTQRMRGDRPWVMTNLKTGAGLDAVVRFIVERGALGPARA"
BASE COUNT          370 a          765 c          794 g          367 t
ORIGIN      
        1 acgacgccga ttgggacacg ctcgagggcc ggcacatggc ggtcaagtgg ttcgtcgact
       61 cgagcctcgc cggcacggcc gcgatcggcc cgcaccagag cgacccccgg accggcgaaa
      121 tcctgtacgg cgcggtgctg atccccgacg tgtgggcgcg catttccggc cagcgcttcg
      181 gcgaagtgct gccgccgcgc gtgcagttgc cgacgggcct gtcgaacctg cttcggtccg
      241 agcagtgctc gtacgcgttc gacgcgctgg agcaggccgc gttctcgttc gaactgctcg
      301 tcgagcgcgg cgcgatcgcg cgcgacagcg aggcggcgcg gcgcttcatc ctcgactcga
      361 tcaaggaggt ggtgatgcat gaggtcggcc acgcgctcgg gctgcgccac aacttcaagg
      421 gctcgagcgc gatcaagttc gatcagctgc gcgacggcgg gttcaccgag aagtacggcc
      481 tgtcgtcgtc gatcatggac tacgtgccgg agaacattcc gctcgacggc gaggcgcgta
      541 ccggccgcct gcagatggac acgatcggca cctacgaccg ctgggcgatc gaatggggct
      601 accggcagtt tgccgccgcc gacgaggcga agttgctgtc cgcgcttgcc gcgcgttcgg
      661 cgaccgatcc gctgctcgcc tatgcaaccg acgacgacgc cggcagcgac ggcgatatcg
      721 gttcagggcc gccgggcctc gatccgcgcg ccaaccgatt cgacctcggc gaggattcgc
      781 tcgtctacta cgagcggcag ctgaaactca cacgcgagct gttcgcgcgc acgcaacggc
      841 gcaagctcga cgccgacgac gactaccgcc tctatcgccg caatctcgag cgcggcctgc
      901 ggcagatgcg cggtgtggcg cccaacgtgg ccaagtatgt cggcgggctg tacgtgaacc
      961 gcgatcgcgc ggcgtcgggc cggccgctgc tgacgccggt cgaggcgcca cgccagcgtg
     1021 cggcgctgaa gctgctgacg gccgaactct tctcgaccgc gagcttcgca ttcgatccgc
     1081 agttcatgca gcgcctcggc gtcgatcagt tcgcgcgcgg caacacgggg ccggatttca
     1141 gcctgccgag cgcggtgctc gatatccagc gaccggtgct cgatcagttg atgtcggaga
     1201 tggtcgcgct gcgcatggcc aatgccgagt cgaagtccgc cgagccgcgc gcgctgctcg
     1261 atttcgccga agtgcagtcg acgctgaccg acgcggtgtg gagcgagctg aagaccggac
     1321 aggacatcgg aaccctgcgc cgcaacctgc agcgcgaaca cctgcgccga ctggccagcg
     1381 cgctggtgcg gccgatctcg accgtggccg ccgacgtccg ctcggtgcag cggcagcagg
     1441 cggaacgact ggtcgcgcaa ctgaagcgcg cattgacgag caagggcaag cgcagcgatg
     1501 tggcgcaggc gcatctggcc gaaagcctgg tcacgctgcg cgaagcgctg gcggcgccga
     1561 tgtaccggac gggagtctga tcgacgctca agcgcgggct ggaccgagcg cgccgcgctc
     1621 cacgatgaag cgcacgaccg cgtcgagccc tgcgcccgtc ttcaggttcg tcatcaccca
     1681 gggccgatcg ccgcgcatgc gctgcgtgtc gtgccgcatc acgtcgaggc gggcgccgac
     1741 atggggcgcc agatcggtct tgttgatcac gagcagatcg cttttagtga tgccgggccc
     1801 gcccttgcgt ggaatcttct cgccgccggc cacatcgatc acgtagatcg tcaggtccga
     1861 caactccggg ctgaaggtcg cggccaggtt gtcgccgccg ctctcgatga agatcaggtc
     1921 gagatcggga aagcgctggc acatgcggtc gatcgcctcg aggttgatcg atgcatcctc
     1981 gcggatcgcc gtgtgcggac agccgcccgt ctccacgccc atgatgcgct cgggcgccag
     2041 cgcctgcgcg tcgaccagca gccgctcgtc ctccttggtg tagatgtcgt tggtgacgac
     2101 cgcgagctga tactgcgagc gcatttgctt gcacagcttt tcgaccagcg tggtcttgcc
     2161 cgagccgacg gggccgccga ccccgacccg aaggggaggg tggttcggag ggccgacgcg
     2221 cagtggattg gatgacacgg agttcaggat cgatacaggc gtgaatactg cgactcgtgg
     2281 cgcgcactcg tcagcg
//
LOCUS       DBNHBY010000121         5903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_73592, whole genome shotgun sequence.
ACCESSION   DBNHBY010000121 DBNHBY010000000
VERSION     DBNHBY010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_73592"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16HB_02955"
     CDS             <1..270
                     /locus_tag="AC16HB_02955"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permuted SH3 domain-containing protein"
                     /protein_id="HIR5605779.1"
                     /translation="VGYSANVEIVLAARDDVLRVPTAAILEGGRVLVVGSDGRLVERK
                     LKTGLANWEWTEVVEGLSAGDKIVTSLERAGVKAGAAVEEEKAAK"
     gene            417..2225
                     /locus_tag="AC16HB_02960"
     CDS             417..2225
                     /locus_tag="AC16HB_02960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR5605780.1"
                     /translation="MAKTNLKLVTKSPPPRAPAKAIEPPSEEYLAEPVPLANYDRSIR
                     AAVARASRGIAWPSLVSSWAEWYWHLAAAPGKQMELISKAQRKAARFALYAAECAQGG
                     AAPCISPLPQDRRFEDPSWQEFPFNLIHQSFLLTQQWIWNATNHVRGVSRHHERVVTF
                     TARQALDVASPTNFVWTNPEVLQKTLETSGQNLMQGAADWWRDFLAFGSGKPRADGLQ
                     FRPGQEVAVTPGKVVLRNDLIELIQYAPATDTVYAEPVLIIPSPILKYYILDLSPHNS
                     MVKYLVGQGHTVFMLSWRNPDARDRELGVDHYLRLGIRAAIDAASAITAQKLHAVGYC
                     LGGTFLAALAASMAGAGDDRLATLTLLAAEVDFTEAGELSIFIDDSQLAYLEDLTWEQ
                     GYLDGKQMSGAFTLLNSNDLIWSRVVRQYLMAEPQKVTDLTAWNVDTTRLPHRMHTEY
                     LRNLYLNNDLASGRMRYDGRSIALSDIRAPIFLVGTERDHVAPWKSVYMLNLYADADI
                     TFCLTSGGHNVGIVNPPEGEAPHRHYRIATKNAADRYVDADQWYARTAPLDGSWWSAW
                     QAWLAERSGARGKPPALGNRDYVAMENAPGTYVLMK"
     gene            2272..2967
                     /locus_tag="AC16HB_02965"
     CDS             2272..2967
                     /locus_tag="AC16HB_02965"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605781.1"
                     /translation="MATAANGALIELAGVERVFRLGDSEVHALHELTLRVAAGEYIAV
                     MGPSGSGKSTLLNILGLLDRPNTGRYLLEGRDVTTLSAEEQARVRSRRIGFVFQSFHL
                     VPRLTAAENVALPMMLAGIAPKERAVRVAQALKDYGLADRSHHRPDQLSGGQRQRVAI
                     ARATIMQPALILADEPTGNLDRATGEEVVRLLEALNARGVTLIVVTHDQALGARARRR
                     VLMEDGAVVGDES"
     gene            2967..4175
                     /locus_tag="AC16HB_02970"
     CDS             2967..4175
                     /locus_tag="AC16HB_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605782.1"
                     /translation="MRAPDILRFATGAAAGSLLRTVLSMLAMSIGVAAVVVLTALGDG
                     ARRFVIDQFSSLGTNLVIVLPGRTQTGGFSPANVVTQTPRDLTVEDARAVLRAPTVAR
                     VAPVSVGNAELTVGGKLRATVIVGTVAEYLDIRHFEMAQGSFLPVEDWSHGTPVMVIG
                     GKVRRELFGAEPAVGKLVRAGDRRFRVVGVLATSGVGLGMNTDELAFVPVSIAQQMFN
                     TNTLFRILIEARHRDAIGTAKAQAQAIIKARHEGEEDVTVITQDAVLATFDRVLGTLT
                     VGVAGIAAISLAVAGILVMNVMLVSVTQRTAEIGLLKALGATPRAIRGAFLTEAALLS
                     AAGGLVGFALGHLGAWVIRYLYPTFPAYPPDWAVLAGLGTAFATGLVFGVMPARRAAR
                     LDPVLALAKR"
     gene            4172..5371
                     /locus_tag="AC16HB_02975"
     CDS             4172..5371
                     /locus_tag="AC16HB_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605783.1"
                     /translation="MIARDAAFLALRAITAHRLRSFLTLLGIGVGIAAVILLTSIGEG
                     IHRYVLAEFSQFGTNVIGIAPGRVEASGGPPGLPASVRPLTFEDARALERIPGVVAVV
                     PTVSGNSEVAGNSRVRRTTIFGGSADMLRVFNVHVRVGQFLPQEEAGVARNFTVLGPK
                     LKEELFGAESALGARVRIGGLQFRVIGVLEAKGQFLGIDLDDTAYIPAARGLELFNRD
                     GLTEIHLSYAEGVPAARVAAGAKKLLTARHGREDFTITTQEEMLKTLGNILDILTMAV
                     AGLGSISLLVGGVGIVTIMTIAVAERTGEVGLMLALGAQRRTILGLFLGEAIVLAALG
                     GGFGLVLGFGLAQGIHAAVPALPVHTPVLYAVLAELVAAAIGLAAGVLPARRASRLDP
                     VESLRAE"
     gene            5555..>5903
                     /locus_tag="AC16HB_02980"
     CDS             5555..>5903
                     /locus_tag="AC16HB_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605784.1"
                     /translation="MGSTTGRLLCTALLASATAFAAPPQAGQDNRYYGGAYNAAYDGR
                     GHYELGRMMHDQRVQLGPRPFFLVEGMDAGPLKDRLMMCKDGPFYRSDFSIAHRGAAL
                     QFPEHTKEAAEAGA"
BASE COUNT          882 a         2021 c         2109 g          891 t
ORIGIN      
        1 gtcggctaca gcgcgaacgt cgagatcgtg ctcgctgcgc gcgacgacgt gctgcgcgtg
       61 cccaccgcgg cgatcctcga aggcggccgt gtgctcgtcg tcggcagcga cggccggctg
      121 gtcgagcgca agctcaagac cgggctcgcg aactgggagt ggaccgaggt cgtcgaagga
      181 ctatccgccg gcgacaagat cgtcacgtcg ctggagcgtg ccggggtgaa agcaggggcg
      241 gcggtggagg aggagaaggc ggcgaagtag gcgatacccg atcttccagc cccctcgaca
      301 ggcgcgaaca ggaagctttc cgagccgcgc ctccaaacca acccgtcgca acttgccccg
      361 cgtcaacctt tccgcgcacc acgctcgcat gctttgaccg tgtcggagaa cctcccatgg
      421 caaagacaaa tctgaaactc gtaacgaagt ccccgccgcc gcgcgcgccg gccaaggcaa
      481 tcgagccgcc cagcgaggaa tacctggccg agccggtgcc gctggccaac tatgaccggt
      541 cgatccgcgc cgcggtggcg cgggccagcc gcggcatcgc gtggccgtcg ctggtgtcgt
      601 cctgggccga gtggtactgg cacctggcgg cggcaccggg caagcagatg gaactcatca
      661 gcaaggcaca gcgcaaggcc gcgcgcttcg cgctgtacgc ggccgagtgc gcacagggtg
      721 gcgccgcgcc ctgcatctcg ccgctgccgc aggaccgccg cttcgaggat ccttcctggc
      781 aggaattccc tttcaacctg atccatcagt cgttcctgct gacgcagcag tggatctgga
      841 acgcgaccaa ccacgtgcgc ggcgtcagcc gccatcacga gcgcgtggtg acgttcacgg
      901 cgcggcaggc actcgacgtg gcgagcccga cgaacttcgt ctggaccaac cccgaggtgc
      961 tgcagaagac gctggagacg agcggccaga acctgatgca gggggcggcc gactggtggc
     1021 gcgacttcct cgcgtttggc tcgggcaagc cgcgcgccga cggcctgcag ttccgccccg
     1081 gccaggaagt cgcagtcaca cccggcaagg tcgtgctgcg caatgacctg atcgaactga
     1141 tccagtacgc gccggcgacc gacaccgtct atgccgagcc ggtgctgatc attccgtcgc
     1201 cgatcctgaa gtactacatc ctcgacctgt cgccgcacaa ctcgatggtc aagtatctgg
     1261 tcgggcaggg gcacacggtg ttcatgctgt cgtggcgcaa ccccgatgcg cgcgaccgcg
     1321 aactcggcgt cgatcactac ctgcggctcg gcatccgcgc cgcgatcgac gcggcgagcg
     1381 cgatcaccgc gcagaagctg cacgcggtcg gctattgcct cggcggcacg ttcctcgccg
     1441 ccctggcggc atcgatggcc ggtgccggcg acgatcgcct cgccacgctg acgctgctcg
     1501 cggccgaggt cgacttcacc gaggccggcg agctgtcgat cttcatcgac gacagccagc
     1561 tcgcgtacct cgaagacctg acgtgggagc agggctatct ggacggcaag cagatgtcgg
     1621 gcgcgttcac gcttctgaac tccaacgacc tgatctggtc gcgcgtggtg cgccagtacc
     1681 tgatggccga accgcagaag gtgacagacc tgaccgcgtg gaacgtcgac acgacgcggc
     1741 tgccgcaccg gatgcacacc gagtatctgc gcaacctgta cctgaacaac gatctcgcgt
     1801 cgggccgcat gcgctacgac ggccgttcga tcgcgttgtc ggacatccgc gcgccgatct
     1861 tcctggtcgg caccgagcgc gatcacgtcg cgccgtggaa gtccgtgtac atgctgaacc
     1921 tgtacgccga cgccgacatc acgttctgcc tcaccagcgg cgggcacaac gtcggcatcg
     1981 tcaatccgcc cgagggcgag gcgccgcacc ggcactaccg gatcgcgacc aagaatgccg
     2041 ccgaccgcta cgtcgatgcc gaccagtggt acgcgcgcac tgcgccgctc gacggctcgt
     2101 ggtggtcggc atggcaggcg tggctcgcgg agcgctccgg cgcgcgcggc aagccgccag
     2161 cgctcggcaa ccgcgactac gtcgcgatgg agaacgcgcc ggggacgtac gtgctgatga
     2221 agtaggggcg cgcggcttat agtccgccgc gatgtttccg cttccgaaat cgtggcgacg
     2281 gcagccaacg gcgcgctgat cgagctcgcc ggcgtcgagc gcgtgttccg gctcggcgac
     2341 agcgaggtgc acgcgctgca cgagctgacg ctgcgcgtgg cggccggcga atacatcgcg
     2401 gtgatggggc cgtcgggctc gggcaagtcg acgctgctga acatcctggg cctgctcgac
     2461 cggccgaaca ccggacgcta cctgctcgaa gggcgcgacg tcacgacgct gtcggccgag
     2521 gagcaggcgc gcgtgcgcag ccggcgcatc ggcttcgtgt tccagagctt ccatctcgtg
     2581 ccgcgcctga ccgcggcgga gaacgtcgcg ctgccgatga tgctggccgg catcgcaccg
     2641 aaagagcgcg ccgtgcgggt ggcgcaggca ctgaaggact acgggctcgc ggatcggtcg
     2701 caccatcgtc ccgaccagtt gtcgggcggg cagcggcagc gcgtggcgat cgcgcgcgcg
     2761 acgatcatgc agccggcgct gatcctcgcc gacgagccga ccggcaacct cgaccgcgcc
     2821 accggcgagg aagtggtgcg gctgctcgaa gcgctgaacg cgcgcggcgt cacgctgatc
     2881 gtcgtcacgc acgaccaggc gctcggcgca cgcgcgcggc ggcgcgtgct gatggaggac
     2941 ggcgccgtgg taggcgacga gagctgatgc gcgcacccga catcctgcgc tttgccaccg
     3001 gcgccgccgc cggcagtctg ctgcgcacgg tgctgtcgat gctggcgatg tcgatcggcg
     3061 tggcggcggt cgtggtgctg accgcgctcg gcgacggcgc gcgccgcttc gtgatcgatc
     3121 agttctcttc cctcgggacc aacctcgtga tcgtgctccc aggacgcacg cagacgggcg
     3181 gcttttcgcc ggccaacgtg gtcacgcaga cgccgcgcga tctgacggtc gaggacgccc
     3241 gcgcggtgct gcgcgcgccc acggtcgcgc gcgtggcgcc ggtgtcggtc ggcaatgcgg
     3301 agctgaccgt cggcggcaag ctgcgcgcga ccgtgatcgt cggcaccgtg gcggagtatc
     3361 tggacatccg ccatttcgag atggcgcagg gcagcttcct gccggtggag gactggtcgc
     3421 acggcacgcc ggtgatggtg atcggcggca aggtgcggcg cgagctgttc ggcgccgagc
     3481 ccgccgtggg caagctcgtg cgcgcgggcg accggcgctt ccgcgtggtc ggcgtgctcg
     3541 ccaccagcgg cgtcgggctc ggcatgaaca ccgacgaact ggcgttcgtg ccggtgtcga
     3601 tcgcgcagca gatgttcaac accaacacgc tgttccgcat cctgatcgag gcgcgccatc
     3661 gcgacgcgat cggcaccgcc aaagcgcagg cgcaggcgat catcaaggcg cgccacgagg
     3721 gcgaggagga cgtcacggtc atcacgcagg acgccgtgct cgcgaccttc gaccgcgtgc
     3781 tcggcacgtt gaccgtcggc gtggccggca tcgccgccat cagcctcgcg gtggcgggca
     3841 ttctggtgat gaacgtgatg ctggtgtcgg tcacgcagcg cacggccgag atcggcttgc
     3901 tgaaggcgct cggcgccacg ccgcgcgcga tccgcggggc gttcctgacc gaggcggcgc
     3961 tgctgtccgc cgccggcggg ctggtcggct tcgcgctcgg ccacctgggg gcgtgggtga
     4021 tccgttacct gtacccgacc tttcccgcgt atccgcccga ctgggccgtg ctggccggac
     4081 tcggcaccgc gttcgcgacc ggcctcgtct tcggcgtgat gccggcgcgt cgcgcagcgc
     4141 ggctcgatcc ggtgctggcg ctggcgaagc gatgatcgcc cgcgacgccg ccttccttgc
     4201 gctgcgcgcg atcaccgcgc accggctgcg cagtttcctg acgctgctcg gcatcggagt
     4261 gggcatcgcg gcggtgattc tgctcacctc gatcggcgag ggcatccacc gctacgtcct
     4321 ggcggagttc tcgcagttcg gcaccaacgt gatcggcatt gcgccggggc gcgtcgaggc
     4381 ctcgggcggg ccgcccgggc tgccggcatc ggtgcggccg ctgacgttcg aggacgcacg
     4441 tgcgctcgag cgcatccctg gcgttgtcgc ggtcgtgccg acggtgtcgg gcaactccga
     4501 ggtcgccggc aacagccgcg tgcggcggac cacgatcttc ggcggcagcg ccgacatgct
     4561 gcgcgtgttc aacgtgcacg tgcgcgtcgg ccagttcctg ccgcaggaag aggcgggtgt
     4621 ggcgcgcaac ttcaccgtgc tcggcccgaa gctgaaggag gaactgttcg gcgcagagag
     4681 cgcgctcggc gcgcgcgtgc gcatcggcgg gctgcagttc cgcgtgatcg gcgtgctcga
     4741 agcgaagggt cagttcctcg gcatcgatct ggacgacacc gcgtacatcc ccgccgcgcg
     4801 cgggctggaa ctcttcaacc gcgacggact gaccgagatc cacctgtcgt acgcggaggg
     4861 cgtgcccgcg gcgcgcgtgg ccgccggtgc gaagaagctg ctcaccgcgc gccacggccg
     4921 cgaggacttc acgatcacca cgcaggagga gatgctgaag accctcggca acatcctcga
     4981 catcctgacg atggcggtcg ccgggctcgg cagcatctcg ctgctggtgg gcggcgtggg
     5041 gatcgtgacg atcatgacga tcgcggtcgc cgaacgcacc ggcgaagtgg gcctgatgct
     5101 ggcgctcggc gcgcagcggc gcacgatcct cggcctgttc ctcggcgagg cgatcgtgct
     5161 cgccgcgctc ggcggcgggt tcggcctcgt actcgggttc gggctcgcgc agggcatcca
     5221 cgccgccgtg cccgcgctgc cggtgcacac gccggttctc tatgcggtgc tggcggaact
     5281 ggtcgccgcg gcgatcgggc tcgctgccgg cgtgctgccg gcgcggcggg cctcgaggct
     5341 cgacccggtc gagtcgctgc gggccgagta gagcgcgcgc cggcgcaggg ccggctggcg
     5401 gatttgccat cgggcgtttg cgcgattgcg cggatcgaac ctgggaattg tcaaggacgc
     5461 gtggtcgcgc tgtgtccaac tgacgtgacc gatgtccgcg cgcgggcgcg accgcgtgct
     5521 cgcggcgtcg aacgtcgttc gagcggagga accgatgggc agcacaacag gcaggctgtt
     5581 atgcacggcc ctgctcgcgt cggccacggc gtttgccgcg ccgccacagg ccgggcagga
     5641 caatcgctat tacggcggcg cctacaacgc cgcctacgac gggcggggcc actacgaact
     5701 cggccggatg atgcacgacc agcgcgtgca gctcggcccg cggcccttct tcctcgtcga
     5761 gggcatggac gcgggcccgc tgaaggaccg gctgatgatg tgcaaggacg ggccgttcta
     5821 tcgcagcgat ttctcgatcg cgcaccgcgg cgcggcgctg cagtttcccg agcacacgaa
     5881 ggaagcggcc gaggccggcg cgc
//
LOCUS       DBNHBY010000122         5225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_141839, whole genome shotgun sequence.
ACCESSION   DBNHBY010000122 DBNHBY010000000
VERSION     DBNHBY010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_141839"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="AC16HB_02985"
     CDS             complement(<1..361)
                     /locus_tag="AC16HB_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR5605785.1"
                     /translation="MKRVDYMHFILARTSARGSRGAVLVVGLIFLVLLMLIGITAYNV
                     ATQEERQAGNARDRIRAIEGAEYALRQCEMQLTGAVVPNFTSTGTNGFYISNPGDLEL
                     YQKPGFSWTSSPTYTVPD"
     gene            complement(358..1416)
                     /locus_tag="AC16HB_02990"
     CDS             complement(358..1416)
                     /locus_tag="AC16HB_02990"
                     /inference="COORDINATES: protein motif:HMM:NF027400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR5605786.1"
                     /translation="MEQLTMRNLNIARAQRGLTIIELMVSVALSLIVLSALTYIYVSS
                     RGAYRSNEAVARVQENGRFAMDWLSREIRGAGFYGCVSRGPAPVVIANNFTGFPMGLQ
                     AIAGAENGAGWTNPTGVTRVAGDVLLLSGMYGTAANILDDNPKAVNANIKYDRCIGLN
                     QNDLVMATNCARSTIMRITNNPENGGCPPPAGFDTATDANNNNNSNFRLDPPYLVSSR
                     AILFKFGAYAYFIGRNPAGRPSLYKTNMDTGGNALTEEVVENIENMDLLFGEDTDNDG
                     FADTYRTASQVANWQSVVSVRVTLIAVSPEAGATQSPQNYYFGANNGGTAVTWQTAAD
                     LRLRQVFVSTVAVRNKLP"
     gene            complement(1404..1838)
                     /gene="pilV"
                     /locus_tag="AC16HB_02995"
     CDS             complement(1404..1838)
                     /gene="pilV"
                     /locus_tag="AC16HB_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HIR5605787.1"
                     /translation="MSLIEVLVAVVILSIGLLGLAGLQAGGLRTGQGALYRGIAAQFA
                     YDMADRMLANVPAVKAGNYNLSLGAAFPVSGGNAAAVNDLNDWMTRMRAALPAADGAV
                     SVAGDLATITVQWDDRRAAVRDANPSAAQTAQIVVTTQLWNN"
     gene            complement(1924..2967)
                     /locus_tag="AC16HB_03000"
     CDS             complement(1924..2967)
                     /locus_tag="AC16HB_03000"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR5605788.1"
                     /translation="MRARPPKPGRRPLTVPTSLRASSRPTASRGDGSGSASAVSSDIG
                     ARFDNPNARPRLTACLSNNRQSGCRISDGRTAVFGGAFPRHFRRVRNPFPSVGGGPPA
                     GLVHFGRVVADDRIAQQPVRARRPLENVARNRSIASSRNATAHCYLRGVTLTELMVVV
                     AIMAVLLGLAVPYMQEFYVSNRLASKSNEFMAALADARNEAMRRGVPVTLRSTAGSQN
                     WGNGWTVCIDSNATTPAGTCASGAANTLRTGMPLNAPLTQYANGTFSTAVTFDANGRV
                     VNTAANSATTYPGIFVICHGGVYASSGRSRSRAILVNAVGRIRVGLDSNGDGTPEGDG
                     ATNIGGTCTAPTP"
     gene            2859..3389
                     /locus_tag="AC16HB_03005"
     CDS             2859..3389
                     /locus_tag="AC16HB_03005"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR5605789.1"
                     /translation="MPIPTHRLSKQLGVTMLEVMLGLSVAGVLASAAVPAFDNLLRSR
                     HLLPTTHELLTALNLARSEALTRGERVAVIARNANWADGWRIFVDRNNNGTHDVDEPV
                     IRDSVPAAPGLSITPNFGITYAGTVLSYESSGRLARPGSHGLVLGRLTLTQGGEVRSL
                     CFASLRVRVVRSATCS"
     gene            3495..4658
                     /locus_tag="AC16HB_03010"
     CDS             3495..4658
                     /locus_tag="AC16HB_03010"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HIR5605790.1"
                     /translation="MDERLRFVARLLEGEKMAELCREFDISRKTGYKIFRRYKDLGLE
                     GLTDRSRRPFRQANKLPMQIETLIVQLKREHPSWGAPKIREKLRRQHDDVQTPAISTV
                     HAVLDRHGLVKHGRRRRHRAQGTGLSQPRRPNDLWCADYKGEFMLADRRYCYPLTVTD
                     FASRYLLSCEALGSTQEAYAFTVFQRVFHEFGLPRAIRTDNGVPFASAHALFGLSRLA
                     VWWLRLGIAIERIKPGCPQQNGRHERMHLTLKKEATKPAAQNLLQQQERFDAFIDCYN
                     HERPHQALEMRYPAELYAASPRPYRGLSELAYPLHDHTVTVTHCGRVCFDRRKINLST
                     VFAGQNVGVKQVAERIWLVSFMHYDLGFFDDETCRLEPTENPFQTKVLPMSPA"
     gene            complement(4696..4771)
                     /locus_tag="AC16HB_03015"
     tRNA            complement(4696..4771)
                     /locus_tag="AC16HB_03015"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4736..4738),aa:Phe,seq:gaa)
BASE COUNT          958 a         1717 c         1586 g          964 t
ORIGIN      
        1 ggtcgggcac ggtgtacgtc ggactcgagg tccagctgaa cccaggcttc tgatagagct
       61 cgaggtcgcc ggggttactg atgtaaaagc cgttcgtgcc cgtgctggtg aagttcggca
      121 ccacggcgcc ggtcaattgc atttcgcact ggcgcagcgc gtactcggcg ccttcgatcg
      181 cacggatcct gtcccgtgca ttgccggcct gacgctcttc ctgggttgcg acgttgtagg
      241 cggtgatccc gatcagcatc aacagcacga gaaagatcaa gcccaccacg agcacggcgc
      301 cgcggcttcc gcgagccgat gtgcgagcca gaatgaaatg catgtagtcg acgcgtttca
      361 tggcagcttg tttcgaacgg cgacagtcga cacgaagacc tgccgcaacc gcaaatccgc
      421 cgcggtctgc caggtcactg ccgtgccgcc gttgtttgcg ccgaagtagt agttctgcgg
      481 tgactgcgtc gcgcccgcct cgggactgac cgcgatcaga gtcacgcgca cgctgaccac
      541 tgactgccag ttggcgacct gagatgcggt ccggtacgtg tctgcgaagc cgtcgttgtc
      601 ggtgtcttcg ccgaacagca gatccatgtt ctcgatgttc tcgacgacct cttcagtcag
      661 cgcgtttcct cccgtgtcca tgttggtctt gtacagcgat gggcggccag ccggatttct
      721 cccgatgaag tacgcatagg cgccgaattt gaacaggatc gcgcgtgagc tgacgaggta
      781 aggaggatcc aggcgaaaat tagaattgtt gttgttgttg gcatcagtcg ccgtgtcgaa
      841 cccggcgggc ggcgggcacc caccattttc gggattattc gtgatgcgca tgatcgtgct
      901 gcgtgcgcag ttcgtcgcca tcaccaaatc gttctggttc agtccgatgc agcggtcgta
      961 cttaatgttg gcgttgaccg ccttcgggtt gtcgtcgagg atgttggcgg ccgtgccgta
     1021 cattcccgac agcagcagaa catcaccggc gacgcgagtt acccccgttg gattggtcca
     1081 gcccgccccg ttctccgcgc ccgcgatcgc ctgcaacccc atcgggaagc cggtgaaatt
     1141 gtttgcgatc accaccggtg ccgggcctcg tgatacgcaa ccatagaagc cggcgccgcg
     1201 gatctcgcgc gacagccaat ccatcgcgaa gcggccgttt tcctgcacgc gcgccacggc
     1261 ctcgttgctc cggtacgcgc ctcgcgaact gacgtagata tacgtgagcg cgcttagcac
     1321 gatcaggctc agcgcgacgc tgaccatcaa ctcgatgatg gtcaagccgc gctgcgcgcg
     1381 ggcgatgttc aaattgcgca tcgtcagttg ttccatagct gcgtcgtcac gacgatctgc
     1441 gcggtctgcg ccgcactcgg gttcgcgtct cgaacggcgg cgcgacgatc gtcccattgc
     1501 accgtgatcg tcgccagatc tccagcgacc gaaaccgcgc catcggccgc aggcagcgcg
     1561 gcgcgcatcc gcgtcatcca atcgttaagg tcgttcaccg ccgcggcatt gcctccgctc
     1621 actggaaacg cggcgccgag cgacaggttg tagttacctg ccttcactgc aggcacattg
     1681 gccagcatgc gatcggccat gtcgtaagcg aactgcgcgg cgatgccgcg gtatagcgcg
     1741 ccctgtcccg tccgcaatcc gcccgcctgc aaacccgcga gaccgagcaa gccgatcgaa
     1801 aggatcacga cggcgaccag cacctcgatc aggctcacgc cacgctcgaa ggctcgcatt
     1861 ccgcaaccac tagggatcaa ttgcttcatc ctcgttcccg ccagttcttt cgtccctgct
     1921 gagctaaggg gttggtgccg tgcatgtgcc gccgatgttc gtcgcgccat caccctccgg
     1981 agtgccgtcg ccgttggaat ccagaccgac ccggatcctg ccgactgcat tgacgagaat
     2041 cgcgcgcgag cgtgagcgtc cgctcgacgc gtagacaccc ccatggcata tgacgaagat
     2101 tcccggatat gtggtcgcgc tgttggcggc ggtgttgacg acccggccgt tggcgtcgaa
     2161 cgtgaccgcc gtcgaaaacg taccgttcgc atactgcgtc aatggcgcgt tcagcggcat
     2221 tcccgtgcgc agcgtattgg ccgcgcccga cgcgcacgtc cccgccggcg tcgttgcatt
     2281 ggagtcgatg catacggtcc agccattacc ccagttctgg gatccggccg tgctcctcaa
     2341 agtcaccgga actcctcggc gcattgcctc gttgcgagca tcggcgaggg cggccatgaa
     2401 ttcattcgac ttcgacgcta ggcgattact gacgtaaaac tcctgcatgt agggcactgc
     2461 cagcccgagc agcaccgcca tgatcgcgac cacgaccatc agttccgtca aagtcacgcc
     2521 gcgcagataa cagtgcgcgg tcgcgtttcg actgctcgcg atcgaacgat tgcgtgcgac
     2581 gttttccaaa ggccgtctcg ccctcaccgg ttgctgagca attctgtcgt cagcgacaac
     2641 cctgccgaag tgaactaagc cggccggcgg accccccccg accgacggga aagggttgcg
     2701 cacgcgcctg aaatgcctcg gaaacgcccc cccaaacacg gcagtccggc cgtcggatat
     2761 tcgacacccc gattgccgat tattcgacag acacgcggtc aggcgaggtc gagcattcgg
     2821 gttgtcgaag cgagcaccga tatccgagga gaccgccgat gccgatcccg acccatcgcc
     2881 tctcgaagca gttgggcgtg acgatgctcg aagtgatgtt gggactgtca gtggccggcg
     2941 tcctggcttc ggcggccgtg cccgcattcg acaacctgct tcgcagccgg cacctcctgc
     3001 cgaccaccca cgaacttctc acggcgctga acctggcccg cagcgaggca ctcacgcgag
     3061 gcgaaagggt cgcggtgatc gcccgcaacg ccaactgggc cgacgggtgg cgaatcttcg
     3121 tcgataggaa caataacggc acgcacgatg ttgacgagcc ggtgatccgt gattcggttc
     3181 cggcggcgcc cggtttgtcc atcacgccca atttcggcat cacctacgcg gggaccgtac
     3241 tttcgtacga aagcagcgga aggctggcac gccccgggag ccacggactg gtgctcggcc
     3301 ggctgacact gacgcaaggc ggtgaagtgc gttcgctctg cttcgcctcg ctgcgcgttc
     3361 gggtcgtccg atcggcgacc tgcagctagc tgtccgtcct gagagagggg taacggttca
     3421 ttccgggcac acaggtgaca ctttcggcct ttgccagaag ggagggcaaa gtgccgtggc
     3481 aggagtgtca caagatggac gagcgcctgc gatttgtcgc ccgactgctg gagggcgaga
     3541 agatggccga gctgtgccgc gagttcgaca tctcacgcaa gaccggctac aagattttcc
     3601 gacgctacaa ggaccttggg ctcgagggcc tgacggaccg cagtcgccgg ccgtttcgac
     3661 aggcgaacaa gctgccgatg cagatcgaga cgctgatcgt gcagctcaag cgcgagcacc
     3721 cgagctgggg cgcacccaag attcgcgaga agctgcgccg gcaacacgac gacgtgcaga
     3781 cgccggcgat cagtaccgtg cacgccgtgc tcgatcgcca cggcctggtc aagcacggcc
     3841 gcagacgccg ccacagggcg cagggcaccg ggctgtcgca gccgcgccgg cccaacgacc
     3901 tgtggtgcgc cgattacaag ggcgagttca tgctggccga tcgtaggtac tgctatccgc
     3961 tgaccgtcac cgacttcgcc agccgctatc tgctcagctg tgaggcgctc ggcagcacgc
     4021 aggaggccta tgcgttcacg gtcttccagc gtgtcttcca tgagtttggc ttgcccaggg
     4081 cgatccgcac cgacaacggt gtgcccttcg ccagcgcgca cgccctgttc ggcctaagca
     4141 ggctcgccgt atggtggctg cgcctgggca tcgcgatcga gcgcatcaag cccgggtgtc
     4201 ctcagcagaa cggccgccat gagcggatgc acctgacgct gaagaaggaa gccaccaagc
     4261 cggccgcgca gaacctgctc cagcagcagg aacgcttcga tgcgttcatc gactgctaca
     4321 accacgagcg cccgcaccag gccctcgaaa tgcgctaccc cgccgaactt tacgcggcgt
     4381 ccccgcgtcc gtatcgagga ctgagcgagc ttgcctaccc gctgcacgac cacacggtca
     4441 cggtcaccca ctgcgggcgc gtctgcttcg accggcgcaa gatcaatctg agcaccgtct
     4501 tcgccggcca gaacgtcggc gtcaaacagg tcgccgagcg catctggctc gtcagcttca
     4561 tgcactacga tctgggattc ttcgacgatg agacatgtcg cctggaaccg actgagaacc
     4621 cgttccagac gaaagtgtta cccatgtctc cggcatgaac cgtcacccat gtgaccggaa
     4681 tacacccatg gggagtggtg gccaagggcg gaattgaacc accgacacaa ggattttcag
     4741 tcctctgctc taccaactga gctacttggc cttgccttgc tacgaccgtc gacaaggatt
     4801 ttgtcttcgg ccgcggggct cgcgccccgc ttaatttcgg ctacgccgaa gttagccttc
     4861 gacgcaactt gtcctgcggc caagttgtca gtcctctgct ctagcaactg agctacttgg
     4921 ccttgccttg aaccggtgac gagaactccg tctccggcca aatgcgaggg cgcgatctta
     4981 ccggaatcgg tcgcctgaat ctcggccgcg acgatggcgg acagaatcgc tgcgagcggc
     5041 cagcgcgtta ccgaacaagg gctggccacg ccgtcacccg gccgctcccg cccgcgccac
     5101 gtagccgcag tactcgcagt tctgcgccgc ggccggtgcg cgctccgatg cgaggcaatc
     5161 cgcgacgtca tgcagcgtcg gctcgaccca caggtcgtcg atcgcgccgt agacggtgag
     5221 cccgg
//
LOCUS       DBNHBY010000123         1980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1021486, whole genome shotgun sequence.
ACCESSION   DBNHBY010000123 DBNHBY010000000
VERSION     DBNHBY010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1021486"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16HB_03020"
     CDS             <1..314
                     /locus_tag="AC16HB_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5605791.1"
                     /translation="AKLGEMDTALDGAALLAYRAAWLRDVRKVRTTKEAAMAKWNATE
                     SAQQVIDRAVQLFGGLGVRKGTAVEGLYREIRALRIYEGATEVQKLVIARDLLHPGNR
                     P"
     gene            311..1969
                     /locus_tag="AC16HB_03025"
     CDS             311..1969
                     /locus_tag="AC16HB_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5605792.1"
                     /translation="MTAHVDTFARDHLPPRGQWPEFLFDRPEYRHPERLNAAVELLDR
                     AVQRGWGKRIALRTLTDGKVLTCTYEQLTAQVNRIARVLIEDLRLVPGNRVLLRGPNN
                     PMMAASWLAAVKAGLVAVGTMPLLRARELQPVIDKARISAALCDVRLKDELAHNLDPA
                     SEFFTPTLKQVLFFNDGRPGSLDARAAAKPVDYEGCDTRRDDVALIAFTSGTTGVPKG
                     TIHFHRDILIMSDAFPQHVLKPEPDDIFCGTPPLAFTFGLGGLLTMPLRAGASVVLAE
                     KLMPDSLLELIAKTRATVLFTAPTMYRQCALLLRETARGLAIPSLRKSVSAGEALPDA
                     TRQLWKEATGLEMIDGIGTTEMIHMFISSAGTDVKRGAIGRVVPGYVAQVVDDDMRPV
                     APGTLGKLAVKGPTGCRYLADPRQASYVKQGWNLPGDTFSMDADGYFTYHARSDDMII
                     SAGYNIAGPEVEGALLLHPAVAECAVIGADDPERGQIVKAYVVPKAGMHADTALVREL
                     QEFVKSQIAPYKYPRAVEFIDALPRTETGKVQRFKLRQRIAATG"
BASE COUNT          330 a          674 c          696 g          280 t
ORIGIN      
        1 aggcgaagct cggcgagatg gacacggcgc tcgatggcgc ggcgttgctt gcctaccgcg
       61 cggcgtggct gcgcgacgtg cgcaaggtgc gaacgacgaa ggaggccgcg atggccaagt
      121 ggaacgcgac cgagtcggcg cagcaggtga tcgaccgcgc cgtgcagctg ttcggcgggc
      181 tcggcgtgag gaagggcacg gcggtcgagg gcctgtaccg cgagatccgc gcgctgcgga
      241 tctacgaggg cgcgaccgag gtgcagaagc tcgtgatcgc gcgcgatctg ctgcatccgg
      301 ggaacaggcc atgaccgccc acgtcgacac cttcgcccgc gaccacctgc cgccgcgcgg
      361 ccagtggccg gaattcctct tcgatcgacc ggagtatcgc cacccggaac ggctcaacgc
      421 ggcggtcgaa ctgctcgacc gcgccgtgca gcgcggctgg ggcaagcgca ttgcgctgcg
      481 cacgctcacc gacggcaagg tgctgacctg cacttacgag cagctgaccg cgcaggtcaa
      541 ccgcatcgcg cgcgtgctga tcgaggacct gaggctggtg ccgggcaacc gcgtgctgct
      601 gcgcggcccg aacaacccga tgatggccgc gagctggctc gcggccgtga aggcgggact
      661 ggtcgcggtc ggcacgatgc cgctgctgcg cgcgcgcgaa ctgcaaccgg tcatcgacaa
      721 ggcgcggatc tccgccgcgc tgtgcgacgt gcgactgaag gacgaactgg cgcacaacct
      781 cgatccggcc tcggagttct tcacgccgac actgaagcag gtgctgttct tcaacgacgg
      841 ccgcccgggc tcgctcgacg cgcgcgcggc cgcgaagccg gtcgactacg aaggctgcga
      901 cacgcgccgc gacgacgtcg cgctgatcgc gttcacgtcc ggcaccaccg gcgtgcccaa
      961 gggaacgatc cattttcacc gcgacatcct gatcatgtcg gacgcgttcc cgcagcacgt
     1021 gctcaaaccg gagcccgacg acatcttctg cggcacgccg ccgctggcgt tcacgttcgg
     1081 gctcggcgga ctgctgacga tgccgctgcg cgccggcgcg agcgtcgtgc tggccgagaa
     1141 gctgatgccg gatagtctgc tcgagctgat cgcgaagacg cgcgccaccg tgctcttcac
     1201 cgcaccgacg atgtaccgcc aatgcgcgct gctgctgagg gaaacggcgc gcggtctggc
     1261 gatcccgtcg ctgcgcaaga gcgtgtcggc gggcgaggcg ctgcccgacg cgacacgcca
     1321 gctgtggaag gaagcgaccg gcctcgagat gatcgatggc atcggcacca ccgagatgat
     1381 tcacatgttc atttcgtcgg cgggcactga cgtgaagcgc ggcgcgatcg gccgggtcgt
     1441 gcccggctac gtcgcgcagg tggtcgacga cgacatgcgg ccggtggcgc cgggaacgct
     1501 cggcaagctc gcggtcaagg ggccgaccgg ctgccggtat ctcgccgacc cgcgccaggc
     1561 gagctacgtg aagcagggct ggaatctgcc cggcgacacg ttctcgatgg acgccgacgg
     1621 ctacttcacc tatcacgcgc gctccgacga catgatcatc tctgccggct acaacatcgc
     1681 cggtcccgag gtcgaaggcg cgctgctgct gcacccggcc gtggccgagt gcgcggtcat
     1741 cggcgccgac gaccccgagc gcgggcagat cgtgaaggcg tacgtcgtgc ccaaggccgg
     1801 catgcacgcc gatacggcgc tggtgcgcga actgcaggaa ttcgtgaagt cgcagatcgc
     1861 cccgtacaag tatccgcgcg ccgtcgagtt catcgacgcg ctgccgcgca cggagaccgg
     1921 caaggtacag cgcttcaagc tgcgccagcg cattgccgcg accggctgag cggcgacagc
//
LOCUS       DBNHBY010000124         6439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_73886, whole genome shotgun sequence.
ACCESSION   DBNHBY010000124 DBNHBY010000000
VERSION     DBNHBY010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_73886"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16HB_03030"
     CDS             <1..513
                     /locus_tag="AC16HB_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5605793.1"
                     /translation="AAERAIEKQVVIDATLDQAWDAWTTRTGITSFFAPDARIEPRVG
                     GAFHIYINPLAEPGMKGADEMRFMALQPKTMISFDWNAPPSLPEARAQRTFVIVRFEP
                     VAEKQTRVSLHHTGWGDGGEWDKAYAYFDRAWGNVLGNLKKRFESGPIDWTEWMEQLR
                     KFQQQSAQKK"
     gene            532..801
                     /locus_tag="AC16HB_03035"
     CDS             532..801
                     /locus_tag="AC16HB_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5605794.1"
                     /translation="MPHVEFAPHLQRHVLCPPQTVDAGTLRAALEAAFAAAPALRAYV
                     LDDQGAVRRHVAVFINGELLHDRGRLERALAPADRIYVAQALSGG"
     gene            849..1943
                     /locus_tag="AC16HB_03040"
     CDS             849..1943
                     /locus_tag="AC16HB_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5605795.1"
                     /translation="MQNLFIATRKGLLVFEADGDMWLLRSTHFLGEPVTSVLVDATGQ
                     RWLAALKLGHFGVKLRLTDDAGATWQEVAAPAYPPKPEAKPGAAADPHAWSLDQVWIL
                     EGFEPMTPERVWAGTNPGGLFRSDDGGRTWMLSESLWNMPERLQWFGGGYDIPGIHSI
                     CVHPNDPDDIVIGVSCGGAWRTRDGGKTWSAGRGMRADFMPPERRDDPIIQDPHRIVQ
                     CASDPNALWTQHHCGIWKSRDRGATWQEVATAIPSNFGFAVAVHPHDPRRAWFVPAMK
                     DDTRVPVNGEFVVSRTSDGGETFEVQRAGLPHRDAFDLVYRHALDITADGHHLAMGST
                     TGSLWVSTGNGESWRTISNHLPPIHAVRFA"
     gene            2046..3005
                     /locus_tag="AC16HB_03045"
     CDS             2046..3005
                     /locus_tag="AC16HB_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5605796.1"
                     /translation="MMRRILALVCAAIASGFALVAQAQNISIGTGGTGGVYYPMGGGI
                     AAVLSKYVPGMQATAEVTGGSVDNLKLIATGKPYIGFTMADAALEAAKGEDKFKGNKV
                     PVRTLMILYPNRMHVVTVEGTGITKFADLKGKRVSTGSPGSATEVMAFRLIEAAGLDK
                     DKDMKRERLGVAESVNAMKDRKIDAFFWVGGLPTAAVTDLAATPVTKIKMIDHADLVP
                     AMNAKWGNLYVQDVIPKETYKGMDADNHQATVMNILVANENMDDKTAYNIVKTIFDKR
                     DDLIAVHKEAANFRLEFQKAAASPVPFHPGAVKYFAEKGIKVN"
     gene            3005..5044
                     /locus_tag="AC16HB_03050"
     CDS             3005..5044
                     /locus_tag="AC16HB_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR5605797.1"
                     /translation="MPAPEAGSPPAAGEVSAEALARAEEYIEEEEGQAHRLTGALGIL
                     VTLVAVAISVFHLYTAYAIVPTQTLRPLHVAMIQALCFLSFPVAKRFRHRIMWWDWLG
                     VLLAIAIVAYLIAGGDDFTDRNTTPNDWDIVFGIALIVLVLEVMRRTTGWVMPVITAT
                     FILYALIGPVLPAPWTHKGYEVGRLIGHMYMTLEGIYGVAVDVSSSLIILFTIFGAFL
                     QYSGAGKFFIDFSFAAMGGKPTGVGRTVVLASFLLGGPSGSGVATTVTIGSVAYPMLA
                     KAGYEKNAAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYLDVLLMATIPTLLYYL
                     ALFLMVEIDARKYGMHTAAFELVDSAWNLTKKYWFHFLSLVSIVVFMMLGYSPVLSVF
                     WATVVTAATSFLHRDCALVPYDVLRGRAPFWRGIYESGLMKALQGGSVGVINVAATCA
                     GAGIIVGVVTLTGLGLKFSGIVIDYAGGSLLLTAIFTALVVWIVGLAVPVTASYIIGA
                     VITAPALIKLGVPDFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQCWK
                     YTVPAFLLPFMFVLDPSGTGLLLTGSIKNLAHADWGSIAWITFIAGVGIAAIAAGFQG
                     WALLKTTLLERWLFIVAGFALVLPGTNSDSVGFGCVAVAIAMQLLRRRRATSAST"
     gene            complement(5183..6181)
                     /locus_tag="AC16HB_03055"
     CDS             complement(5183..6181)
                     /locus_tag="AC16HB_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5605798.1"
                     /translation="MSKRRPPRLSLDLLRGFNAAARHLSFTRAAQELSLTQSAISRGI
                     RTLEQQLGQPLFHRVHRALQLAPAGQKLLQTTEEVFARIDDVAAQLIDSKRVLAITTT
                     TALASLWLAPRLPRFNRLHAGIDVRIVASDAPVDLEREQIDIAIPFVPHGAHPPPTGE
                     RLLECEFFPVCSPALANDPAHPLRTPADLAHHVWLDHDTIRDGRPWSKWDVWFKGTGT
                     SPVKPVSMLRFSHGDQMIAATLDGSGVAMGGRPHLARQLRDGVLCAPFGPEAVAPVGA
                     FFVHIRPAAASQDAVQAFVAWLRSEARRDNELTLAVPSRTRRAPRAPVHGPRARAS"
BASE COUNT         1086 a         2061 c         2203 g         1089 t
ORIGIN      
        1 gcggccgagc gcgcgatcga gaagcaggtg gtgatcgacg ccacgctcga ccaggcgtgg
       61 gacgcgtgga ccacccgcac agggattacg agcttctttg cgccggacgc gcggatcgag
      121 ccgcgcgtcg gcggtgcgtt ccacatctat atcaatccgc tcgccgagcc cggcatgaag
      181 ggcgcagacg agatgcgctt catggcgctg cagcccaaga cgatgatctc gttcgactgg
      241 aacgcgccgc ccagtctgcc tgaagcccgc gcgcaacgga ccttcgtgat cgtgcgcttc
      301 gagccggtcg cggagaagca gacgcgcgta tcgctgcacc acaccggttg gggcgacggt
      361 ggcgaatggg acaaggcgta cgcatacttc gaccgcgcct ggggcaacgt gctcggcaac
      421 ctgaagaagc gcttcgagtc cgggcccatc gactggaccg agtggatgga gcagttgcgc
      481 aaattccagc agcagtcggc gcagaagaag tagcgtcacc aggctcgaac cttgccgcac
      541 gtcgaattcg cgccccatct gcagcggcac gtcctgtgcc cgccgcagac ggtcgacgcc
      601 ggcactctgc gcgcggcgct cgaagcggcc ttcgctgccg cgccggcact gcgtgcatac
      661 gtgctcgacg accagggcgc ggtgcgccgg cacgtggccg tgttcatcaa cggcgaattg
      721 ctgcacgacc gcgggcgcct cgaacgcgcg ctggcacccg cggaccgcat ctacgtcgca
      781 caggccttgt ccgggggctg acgccggacc acactcgccg ccgcgcagat cgacgaccgg
      841 aggtccagat gcagaacctg ttcatcgcca cccgcaaggg cctgctggtg ttcgaggccg
      901 acggcgacat gtggctgctg cgcagtacgc attttctcgg cgagccggtc acgtcggtgc
      961 tggtcgacgc gaccgggcag cgctggctgg cggcgctcaa gctcgggcac ttcggcgtga
     1021 aactcaggct gaccgacgac gccggcgcaa cgtggcagga ggttgcggcg cccgcgtatc
     1081 cgcccaagcc cgaagcaaag cccggcgcgg cggcagaccc gcacgcgtgg tcactcgacc
     1141 aggtgtggat acttgaaggc ttcgagccga tgacgcccga gcgcgtctgg gccggaacga
     1201 atccgggcgg cctgttccgc tccgacgacg gcggccggac gtggatgctg agcgaaagcc
     1261 tgtggaacat gcctgagcgc ttgcagtggt tcgggggcgg ctacgacatc cccggcatcc
     1321 actcgatctg cgtgcacccg aatgatcccg acgacatcgt gatcggcgtc tcgtgtggcg
     1381 gcgcgtggcg tacgcgcgat ggcggcaaga cctggagcgc gggccgcggc atgcgggccg
     1441 atttcatgcc gccggaacgg cgcgacgatc cgatcatcca ggatccgcac cggatcgttc
     1501 agtgcgcgtc tgacccgaac gcactgtgga cgcagcacca ttgcggcatc tggaaatcgc
     1561 gcgatcgcgg cgcgacgtgg caggaagtgg cgacggcgat tccgagcaac ttcggcttcg
     1621 cggttgccgt acatccgcac gatccgcgca gggcctggtt cgttcccgca atgaaggacg
     1681 acacgcgggt gcccgtcaac ggcgagttcg tggtttcgcg caccagcgac ggcggcgaga
     1741 ccttcgaagt gcagcgggca gggttgccgc atcgcgatgc gttcgatctg gtctatcgcc
     1801 acgcgctcga catcaccgcc gacggccacc atctcgcaat gggctcgacc accggttcgc
     1861 tgtgggtttc cacggggaac ggggagtcct ggcgcacgat cagcaatcac ctgccaccga
     1921 tccatgcggt ccggttcgcc tgaacgcctc gcgtcgactt gaccgcgctg gccggaccgg
     1981 gtcagcatcc gtcattgcgc gcagccaccc ggccgcgcgt ccgacaacat gacggagata
     2041 cagacatgat gcgaaggatc ctggccctgg tctgcgcggc gatcgcgagc ggtttcgcgc
     2101 tcgttgcaca ggcacagaac atctcgatcg gcaccggcgg caccgggggt gtgtactacc
     2161 cgatgggggg cggcatcgcc gccgttctct cgaagtacgt gccgggcatg caggcgactg
     2221 ccgaggtgac cggcggctcg gtcgacaact tgaagctgat cgccaccggc aagccctaca
     2281 tcggcttcac gatggccgat gcggcgctcg aggcggcgaa gggcgaggac aagttcaagg
     2341 gcaacaaggt cccggtgcgc acgctgatga tcctctaccc gaaccgcatg cacgtggtca
     2401 cggtcgaggg cacgggcatc accaagttcg ccgacctgaa aggcaagcgc gtgtcgaccg
     2461 gttcgcccgg cagcgcgacc gaggtgatgg cattccgcct gatcgaggca gcggggctcg
     2521 acaaggacaa ggacatgaag cgtgagcggc tcggcgtggc cgaatcggtc aacgcgatga
     2581 aggacaggaa gatcgacgcc ttcttctggg tcgggggact tccgaccgcg gcggtgaccg
     2641 acctcgctgc cacaccggtg acgaagatca agatgatcga ccatgcggat ctggtgccgg
     2701 cgatgaacgc caagtggggc aatctgtacg tgcaggacgt gatcccgaag gaaacctaca
     2761 agggcatgga cgccgacaac caccaggcca cggtgatgaa catcctggtc gccaacgaga
     2821 acatggacga caagaccgcc tacaacatcg tcaagacgat cttcgacaag cgcgatgacc
     2881 tgatcgccgt gcacaaggaa gccgccaact tccggctcga gttccagaag gccgccgctt
     2941 cgccggtgcc gttccatccc ggcgcggtga agtacttcgc tgagaaaggc atcaaggtca
     3001 actagtgccc gcgcccgaag ccggttcgcc gcccgcagcc ggcgaagtca gcgcggaagc
     3061 gctcgcgcgc gccgaggagt acatcgagga ggaggagggc caggcgcacc ggctcaccgg
     3121 cgcgctcggc atcctcgtca cattggtcgc ggtggcgatc tcggtgtttc acctgtacac
     3181 cgcgtacgcg atcgtgccga cgcagacgtt gcggccgctg cacgtggcga tgatccaggc
     3241 gctgtgcttc ctgtcgtttc cggtcgcgaa gcggtttcgc caccggatca tgtggtggga
     3301 ctggctgggc gtgctgctgg cgattgcgat cgtcgcgtat ctcatcgcgg gcggcgacga
     3361 cttcaccgac cgcaacacca cgcccaacga ctgggacatc gtgttcggca tcgcgctgat
     3421 cgtgctggtg ctcgaggtaa tgcggcgcac gaccggctgg gtgatgccgg tcatcacggc
     3481 aacgttcatc ctgtatgcgc tgatcggacc ggtgctgccg gcgccgtgga cgcacaaggg
     3541 ctacgaggtc ggccggctga ttggccacat gtacatgacg ctcgagggca tctacggcgt
     3601 cgcggtcgac gtgtcgtcgt cgctgatcat cctgttcacg atcttcggcg cgttcctgca
     3661 gtactcgggc gcgggcaagt tcttcatcga cttctcgttc gccgcgatgg gcggcaagcc
     3721 caccggcgtc ggccgcacag tcgtgctggc atccttcctg ctcggcgggc cgtcgggctc
     3781 cggcgtggcg acgacggtca cgattggctc ggtcgcgtat ccgatgctgg ccaaggccgg
     3841 ctacgagaag aacgccgccg gcggattgct cgccgccggc gggctgggcg ccatcatctc
     3901 gccgccggtg ctgggcgccg ctgcgttcct gatcgccgag ttcctgaaga tctcatacct
     3961 cgacgtgctg ctgatggcga cgatcccgac gctgctgtat tacctcgcgc tgttcctgat
     4021 ggtcgagatc gacgcgcgca agtacggcat gcacaccgcg gcgttcgaat tggtcgactc
     4081 ggcgtggaac ctgacgaaga agtactggtt tcacttcctg tcgctggtgt cgatcgtcgt
     4141 cttcatgatg ctcggctact cgccggtgct gtcggtgttc tgggccaccg tcgtgaccgc
     4201 ggccaccagc ttcctgcacc gcgactgcgc gctcgtgcct tacgacgtgc tgcgcggccg
     4261 cgcgccgttc tggcggggca tctatgaatc ggggctgatg aaggcgctgc agggcgggtc
     4321 ggtcggcgtg atcaacgtcg cggcaacctg cgccggcgcc ggcatcatcg tcggcgtggt
     4381 cacgctgacc gggctgggcc tgaagttcag cggcatcgtg atcgactacg cgggcggctc
     4441 actgctgctc accgcgatct tcaccgcgct cgtggtgtgg atcgtcggcc tcgcggtgcc
     4501 ggtgaccgcc tcctacatca tcggcgcggt gatcaccgcg ccggcgctga tcaagctcgg
     4561 cgtgcccgat ttcgcggcgc acatgttcat cttctactac gcggtactgt ccgaggtctc
     4621 gccgccgacc gcgctgtcgc cgttcgccgc cgctgcaatc accggcggcg atccgtacaa
     4681 gacgacgctg cagtgctgga agtacacggt gccggcgttc ctgctgccgt tcatgttcgt
     4741 gctcgacccg agcggcacgg gtctgctgct gaccggatcg atcaagaacc tcgcgcacgc
     4801 cgactggggg tcgatcgcgt ggatcacctt catcgccgga gtcggcatcg ccgcgatcgc
     4861 ggcgggcttt cagggctggg cgctgctgaa gacgacgttg ctggagcggt ggcttttcat
     4921 cgtcgccggg ttcgcgttgg tgctgcccgg cacgaacagc gactcagtcg ggttcgggtg
     4981 cgtggcggtg gcgattgcga tgcagttgct gaggcgccgg cgcgctactt ccgcgtcgac
     5041 gtgaagagcg gaagagggtc acggccccgc cggctttcgg accacgcggt aagcccgaga
     5101 gatcctttgg cgtccttcga atcgtccgaa tgcggtcgtt caggctgctt cggaagcagg
     5161 cgctcaacgt tccggttggg cgtcacgatg cgcgagcgcg gggtccatga accggcgcac
     5221 gaggtgcacg ccgtgttcgg ctggggactg ccagtgtcag ttcgttatcg cggcgcgctt
     5281 cgctccgcaa ccatgcgacg aatgcctgca cagcatcttg cgaggctgcg gcaggcctga
     5341 tgtgaacgaa gaacgcgccc acgggggcga cggcctcggg cccaaatggg gcacagagca
     5401 ctccatcccg aagctgtcgg gcaaggtgcg ggcggccacc catggcaaca ccgctgccgt
     5461 cgagcgttgc cgcgatcatc tgatccccgt gcgagaaccg gagcatgctc acgggcttca
     5521 cgggcgaagt cccggttccc ttgaaccaaa catcccactt ggaccacggg cggccgtcgc
     5581 ggatcgtgtc gtggtccaac cacacgtgat gcgccagatc ggctggcgtg cgtagcgggt
     5641 gcgccgggtc attcgcaagc gccggcgagc agaccgggaa gaactcgcac tcgagcaacc
     5701 gttcaccggt gggtggagga tgtgcgccgt gcgggacgaa gggaatggcg atgtcgatct
     5761 gctcgcgctc caggtcgacc ggagcgtcgc tggcgacgat ccggacgtcg atgcctgcat
     5821 gcagccggtt gaagcgaggg agcctcggcg caagccagag ggaggcgagc gcggtcgtgg
     5881 tcgtgatcgc cagaacccgc ttcgaatcga tcagttgagc cgcaacgtca tcgatccgcg
     5941 cgaatacctc ctcggtcgtc tgcagaagct tctggcccgc aggggcgagc tggagcgcgc
     6001 ggtgcacccg atgaaacagc ggctgtccga gctgttgttc gagcgtcctg atccctcgac
     6061 tgattgccga ttgcgtaagc gacagttctt gcgcggcgcg ggtgaagctc aagtggcgag
     6121 cggcggcgtt gaagccacgc agaagatcga gcgacaaccg aggcgggcgg cgcttactca
     6181 tcgtcggctc ctgaggcgaa tcgacggcga acgcgttgac tgggacagat tatcactacc
     6241 gtgtagcgcg gatatgcgta gagctctcgg ctccgcgacg cataacgatt gagctcagcc
     6301 gcgtgtggac gccgcaggcc gtagcaggtc gtgtcagagt cgagcgccgc cgggattgcg
     6361 atggaagggg caaagatttc aaaggccgca atgtccggtg ctcgcgaacg acggcctgca
     6421 cgctcactca tcggccgtg
//
LOCUS       DBNHBY010000125         2399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_6492, whole genome shotgun sequence.
ACCESSION   DBNHBY010000125 DBNHBY010000000
VERSION     DBNHBY010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_6492"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /gene="metG"
                     /locus_tag="AC16HB_03060"
     CDS             <1..1479
                     /gene="metG"
                     /locus_tag="AC16HB_03060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR5605799.1"
                     /translation="AWTAGRNRRGEPHLQPEVYAKTQEWIGAGGKGLADWDISRDAPY
                     FGIPIPDTKDKFFYVWLDAPIGYLASLKAYFDQGKARAKGETRSYDEFVAASDVEQVH
                     FIGKDIVYFHTLFWPAMLKFSGRKVPDSVYVHGFITVSGEKMSKSRGTGIDPLRYLEV
                     GMNPEWFRYYVAAKLNAHVEDIDFNPDDFVARVNSDLIGKYVNIASRAAGFLVKHFDG
                     RLAATEDPSGAPAGASAVALASRDEIGAHLDGRDFGKALREAMRIADFANEHFDKHKP
                     WELARDPAQRPLLQAVCSDVLNAFRALTIYLAPVLPATASRVARELFGMTRDFVWDDL
                     ARPTERVLPYKHLMARVEAKQIDALFEPPAPPEVAVPLPGGEAIAATITIDDFAKLDL
                     RIAKIVDAAYVEGSDKLLRLTLDAGEGRQRTVFSGIKSAYRPEDLVGRLTVLVANLAP
                     RKMKFGVSEGMVLAASHADEKAQPGLYLLDPHPGALPGMRVR"
     gene            complement(1480..2385)
                     /locus_tag="AC16HB_03065"
     CDS             complement(1480..2385)
                     /locus_tag="AC16HB_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR5605800.1"
                     /translation="MSVKFWIAGLFAASAVYVHLRGRVRHGFARQLTDHSTILAPINS
                     LLYLSSKVPAQPFLDAADFPELAPLQANWQKIRDEAMQLYDVGRITVATGYNDVGFNS
                     FFKTGWTRFYLTWYGESLPSALAACPQTMALLQSIPSIKAAMFAVLPPGATLPRHRDP
                     YAGSLRYHLGLRTANSPACYIDVDGERRHWRDGEVLMFDETFIHYARNETDVQRVILF
                     CDVERPLNNPLARAFNRLVRATMMNASASANVPGERIGGVNRLFARVIGIRLWGKRLK
                     ARSRFWYYALKYAVLAALVALFVLA"
BASE COUNT          416 a          789 c          843 g          351 t
ORIGIN      
        1 gcgtggaccg ccggcaggaa ccgccgcggc gagccgcacc tgcagccgga ggtgtacgcg
       61 aagacgcagg agtggatcgg cgcgggcggc aagggcctgg cggactggga catctcgcgc
      121 gatgcgccct acttcggcat accgatcccc gacacgaagg acaagttctt ctacgtgtgg
      181 ctcgacgcgc cgatcggcta cctggcgtcg ctgaaggcgt acttcgacca gggcaaggcg
      241 cgcgccaagg gagagacgcg cagctacgac gagttcgtcg cggcgtccga cgtcgagcag
      301 gtgcacttca tcggcaagga catcgtgtac ttccacacgc tgttctggcc ggcgatgctg
      361 aagttctccg gccgcaaggt gcccgacagc gtgtacgtgc acggcttcat caccgtcagc
      421 ggcgaaaaga tgagcaagag ccgcggcacc gggatcgatc cgctgcgcta tctcgaggtc
      481 ggcatgaacc ccgagtggtt ccgctactac gtggcggcaa agctgaacgc gcacgtcgag
      541 gacatcgact tcaacccgga cgacttcgtc gcgcgcgtga acagcgacct gatcggcaag
      601 tacgtgaaca tcgcgagccg ggcggcgggt ttcctggtca agcacttcga cggccgcctg
      661 gcggcgaccg aagatccgtc cggcgcaccg gcgggcgcca gcgcggtggc gctggcctcg
      721 cgcgacgaga tcggcgcgca cctggacggc cgcgatttcg gcaaggcgct gcgcgaggcg
      781 atgcggatcg cggatttcgc caacgagcac ttcgacaagc acaagccgtg ggagctggcg
      841 cgcgacccgg cgcagcgccc gctgctgcag gcggtgtgca gcgacgtgct caacgccttc
      901 cgcgcgctga ccatctatct cgcgccggtg ctgccggcca cggcgagccg cgtggcgcgt
      961 gaactgttcg gcatgacgcg cgatttcgtc tgggacgacc tcgcgcgacc gaccgagcgc
     1021 gttctgccgt acaagcacct gatggcgcgc gtcgaggcga agcagatcga tgcgctgttc
     1081 gagccgcccg cgccgcccga agtcgcggtg cctctgccgg gcggcgaggc gatcgccgcg
     1141 acgatcacga tcgacgattt cgcgaagctc gacctgcgga tcgcgaagat cgtcgatgcc
     1201 gcttacgtcg agggctcgga caagttgctg cggctgacgc tcgacgccgg tgaagggcgt
     1261 cagcgcaccg tgttctccgg gatcaagtcc gcctaccggc ccgaggacct cgtcggcagg
     1321 ctcacggtgc tggtcgcgaa tctggcgccg cgcaagatga agttcggcgt cagcgaaggc
     1381 atggtgctcg cggcgagcca tgccgacgag aaagcgcagc ccggcttgta cctgctcgat
     1441 ccgcatccgg gcgcgctgcc cgggatgcgc gtccgatagt caggcgagga cgaacaacgc
     1501 gacgagcgcg gccagcaccg cgtacttcag cgcgtagtac cagaagcggc tgcgcgcctt
     1561 cagccgcttg ccccacaggc ggatcccgat cacgcgcgcg aacaggcggt tcacgccgcc
     1621 gatgcgctcg cccggcacgt tggcgctggc cgacgcgttc atcatcgtcg cgcgcaccag
     1681 ccggttgaac gcgcgcgcca gcggattgtt cagcggccgc tcgacgtcgc agaacaggat
     1741 cacccgctgc acgtcggtct cgttgcgcgc gtagtggatg aaggtctcgt cgaacatcag
     1801 cacttcgccg tcgcgccagt ggcggcgctc gccgtcgacg tcgatatagc aggccggcga
     1861 attggccgtg cgcaggccga ggtggtagcg cagcgagccg gcgtacggat cgcggtggcg
     1921 cggcaatgtg gcgcccggcg gcagcaccgc gaacatcgcc gccttgatcg aaggaatcga
     1981 ttgcagcagc gccatcgtct gcggacacgc agcgagcgcc gacggcagcg actcgccgta
     2041 ccaggtcagg tagaagcgcg tccagccggt cttgaagaag gagttgaagc cgacgtcgtt
     2101 gtagccggtc gccaccgtga tgcggccgac gtcgtagagc tgcatcgcct cgtcgcggat
     2161 cttctgccag ttggcctgca gcggcgccag ctcgggaaag tcggccgcgt cgagaaaggg
     2221 ctgcgccggc accttcgacg acaggtacag cagcgagttg atcggcgcca ggatcgtcga
     2281 gtgatcggtc aactggcgcg cgaaaccgtg ccgcacgcgg ccgcgcaggt ggacgtacac
     2341 cgccgatgcc gcgaacaggc cggcgatcca gaacttgacg gacaggagga gctgcacgg
//
LOCUS       DBNHBY010000126         2174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1360248, whole genome shotgun sequence.
ACCESSION   DBNHBY010000126 DBNHBY010000000
VERSION     DBNHBY010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1360248"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16HB_03070"
     CDS             complement(<1..704)
                     /locus_tag="AC16HB_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5605801.1"
                     /translation="MEKVWLKQYPPGIPAEVNIDEYASLKEILEKSCAKFSELPAYGN
                     MGHSITFRELDRASSAFGAYLQKVVGMKKGDRIAIMLPNLLQYPVALFGALRAGFTVV
                     NTNPLYTARELEHQLKDSGATAILVLENFASTLQQVLGRTQVRTVITTQIGDMFPPVK
                     ALITNLVVKHVKKMVPEWKIANAISFPAVLRLGREHALADVQLGHDDIAFLQYTGGTT
                     GVAKGAMLTHGNMVANV"
     gene            complement(736..1911)
                     /gene="maeA"
                     /locus_tag="AC16HB_03075"
     CDS             complement(736..1911)
                     /gene="maeA"
                     /locus_tag="AC16HB_03075"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HIR5605802.1"
                     /translation="MVREVLELDHDPRKDLLCCRDELLDQAVVGLAGQSRLAGPQYDA
                     LVEEFVTAAQEVFPGVLVQFEDFGNANAFRLLARYRDRLPCFNDDIQGTGAMALAGVL
                     SALCISGKPLTEQRFLFFGAGEANIGIGTIVAAALERAGLSATDARRHCWYIDSKGLV
                     TTQRTDLAAHKQPFAQDRETMPDLRATVEAVQPTFLIGASGQAGAFTEPVLAAMARLN
                     QRPVIFALSNPTAKAECTAEQVYRATQGRAIFASGSPFPPVTFEGRTFVTGQGNNSHV
                     FPGIGLAVLACGLTRVTDEMYFAAAQTLAGQVGADDLAAGCIFPPASRMREVSAAVAI
                     AVAEVGYAQGVARVARPADLGAHIRASRYHARYADSRTFQPETSTGSMRALGAVATL"
     gene            1834..>2174
                     /locus_tag="AC16HB_03080"
     CDS             1834..>2174
                     /locus_tag="AC16HB_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR5605803.1"
                     /translation="MVEEFVAATQEVFPGVVVQFEDFANHNAFRLLERYRNRVCCFND
                     DIQGTGAMALAGVLSALRISGKPLTEQRFLFFGAGEANIGIGTIVAAAFERAGLSAAD
                     ARRHCWYIDSKG"
BASE COUNT          356 a          731 c          708 g          379 t
ORIGIN      
        1 acgttcgcga ccatgttgcc gtgagtcagc atcgcaccct tggccacgcc cgtcgtgccg
       61 cccgtgtact gcaggaacgc gatgtcgtcg tggccgagtt gaacgtccgc cagcgcgtgt
      121 tcgcgaccga ggcgcagcac cgccgggaac gagatcgcgt tcgcgatctt ccactccggc
      181 accatcttct tcacgtgctt gaccaccagg ttggtgatca gcgccttgac gggcgggaac
      241 atgtccccga tctgcgtcgt gatcaccgtg cggacctgcg tacgcccgag cacctgctgc
      301 agcgtgctgg cgaagttttc cagcacgagg atcgcagtgg cgccggaatc cttcagctgg
      361 tgctccagct cacgcgcggt atagagcgga ttggtattga cgaccgtgaa ccccgcacgc
      421 agcgcaccga agagcgccac cggatactgg agcaggttcg gcagcatgat cgcgatgcgg
      481 tcgcccttct tcatgccgac gaccttctgc agataggcgc cgaacgcgct gctggcgcga
      541 tcgagttcgc ggaaggtgat cgagtggccc atattgccgt aggccggcag ctcgctgaac
      601 ttggcgcagg acttctccag gatttccttc agcgaggcgt attcgtcgat attgacttcg
      661 gccgggattc ccggcgggta ttgcttcagc cagacttttt ccatgatcca ctcccagaca
      721 acgaaactct ttcttttata aggttgccac cgcaccgagc gcgcgcattg atccagtaga
      781 tgtttcgggc tgaaaggtcc gcgaatccgc atagcgcgcg tggtagcggc tggcgcggat
      841 gtgcgcaccg aggtcggccg gtcgcgcgac gcgcgccacc ccctgtgcat agcccacctc
      901 ggccaccgca atcgccacgg cagccgacac ctcccgcatg cgcgacgccg gcgggaagat
      961 gcagcccgcc gccaggtcat cggcgccgac ctgccctgcc agcgtttgcg cggcggcgaa
     1021 atacatctcg tcggtcacgc gcgtcagccc gcaggcgagc accgcgaggc cgattcccgg
     1081 aaagacgtgc gagttgttgc cttgcccggt cacgaacgtg cgaccctcga aggtcaccgg
     1141 cgggaacggg ctgccgctgg caaagatcgc tcgcccctgt gtggcgcggt agacctgctc
     1201 ggcggtgcac tccgctttcg cggtcgggtt tgacaacgcg aagatcaccg gccgctggtt
     1261 cagccgcgcc atcgctgcaa gcaccggctc ggtgaacgcg cccgcctgcc cgctggcgcc
     1321 gatcaggaac gtgggctgga ccgcttccac cgtggcgcgc aggtctggca tcgtttcgcg
     1381 gtcctgcgcg aatggctgct tgtgggcggc cagatcggtc cgctgcgtcg tcaccagccc
     1441 cttgctgtcg atgtaccagc aatgccgccg cgcgtcggtc gcggacaggc ccgcgcgctc
     1501 gagcgccgcc gcgacgatcg tgccgatccc gatgttcgcc tcgccggcac cgaagaaaag
     1561 gaaccgctgc tccgtgagcg gcttgccgga gatgcacagc gccgacagca cgcccgcgag
     1621 tgccatcgca ccggtgccct gaatgtcgtc gttgaagcac ggcaagcggt cgcgataacg
     1681 cgccagcagc cggaaggcgt tggcgttgcc gaaatcctcg aactgcacca gtacgcccgg
     1741 aaatacttcc tgcgcggcgg tgacgaactc ctcgaccagt gcgtcgtact gcggtccggc
     1801 cagccgcgac tgaccggcga ggcctacgac agcctggtcg aggagttcgt cgcggcaaca
     1861 caggaggtct ttccgggggt cgtggtccag ttcgaggact tcgcgaacca caacgcgttc
     1921 cggctgctcg agcggtaccg gaaccgcgtc tgctgcttca acgacgacat tcagggcacc
     1981 ggtgcgatgg cactcgcggg cgtgctgtcg gcgctgcgca tctccggcaa accgctcacc
     2041 gagcagcggt tcctgttctt cggtgccggc gaggcgaaca tcgggatcgg cacgatcgtt
     2101 gcggcggcgt tcgagcgcgc aggcctgtcc gcggccgacg cgcggcggca ttgctggtac
     2161 atcgacagca aggg
//
LOCUS       DBNHBY010000127         1531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_277446, whole genome shotgun sequence.
ACCESSION   DBNHBY010000127 DBNHBY010000000
VERSION     DBNHBY010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_277446"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16HB_03085"
     CDS             <1..260
                     /locus_tag="AC16HB_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605804.1"
                     /translation="VADLYAAVMEGAVERVRPKMMTVVAIMGGLLPIMWSTGTGSEVM
                     RRIAAPMVGGMVSSTVLTLVVIPAIYTLVMQWKLKRTESPT"
     gene            275..601
                     /locus_tag="AC16HB_03090"
     CDS             275..601
                     /locus_tag="AC16HB_03090"
                     /inference="COORDINATES: protein motif:HMM:NF023036.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HIR5605805.1"
                     /translation="MKTLHFLLAAALFAPALPAFAQHSGHQPAATAAKTELSDGEVRR
                     IDKAKGTVLLKHGEIKNIGMGAMTMSFKFKDAKMAEGLAVGDKVKFAAEQKGEDLIVT
                     QIVKAP"
     gene            complement(588..>1531)
                     /locus_tag="AC16HB_03095"
     CDS             complement(588..>1531)
                     /locus_tag="AC16HB_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5605806.1"
                     /translation="LALVPSLAFVAAAAVLLGLGYGPITPASSHVLARTATPRNMALT
                     LSIKQTGVPAGMALAGIVVPPLAVALGWRIAVLIVAALCAAVAVAAQPLRSELDADRD
                     RRARISLAQLAAGLRLVITTPRLRTVAAVSFVYSGMQMCTTGFIVAYLADELGVPLVA
                     AGVGLTTASLTGVAARIAWGALADRWLSPHMTLALLGALMAAFSCVVALFTHAWPFAA
                     MLVVLAALGATAIGWNGVYLAEVARVAPDGQAGLATGGCLFFTFVGVVVCPALFGWAQ
                     RVSGSYAVSFAAAAAVCGAVALWLFAGGRAGAPATAP"
BASE COUNT          290 a          540 c          520 g          181 t
ORIGIN      
        1 cggtcgccga cctgtacgcg gcggtgatgg aaggcgcggt cgagcgcgtg cggccgaaga
       61 tgatgaccgt ggtcgcgatc atgggggggc tcctgccgat catgtggtcg accggcaccg
      121 ggtccgaagt gatgcgccgg atcgccgcgc ccatggtcgg cggcatggtg tcgtcgacgg
      181 tgctcacgct cgtcgtcatc ccggcgatct acacgctcgt tatgcaatgg aaactgaaac
      241 gcaccgaatc cccaacctga agacggagcc atcgatgaag accctgcatt tcctcctcgc
      301 cgccgccctg ttcgcgcctg ccctgccggc gttcgcgcag cacagcgggc atcagcccgc
      361 cgcgaccgcg gcgaaaaccg aactctcgga cggtgaggtg cgccgaatcg acaaggccaa
      421 aggcaccgtt ctgctgaaac atggcgagat caagaacatc ggcatgggcg cgatgacgat
      481 gagcttcaag ttcaaggacg ccaagatggc cgaaggcctc gccgtcggcg acaaggtgaa
      541 attcgctgcc gagcagaagg gcgaggatct gatcgtcacg cagatcgtca aggcgccgta
      601 gccggtgcgc cggcgcggcc gccggcgaac agccacagcg cgaccgcgcc gcagacggca
      661 gccgccgcgg cgaaactcac ggcgtagctg ccgctgacgc gttgcgccca gccgaaaagt
      721 gccggacaca cgacgactcc gacgaaggtg aagaacaggc agccgccggt ggccagcccc
      781 gcctggccgt ccggtgcaac gcgcgcgact tccgcgaggt aaacgccgtt ccagccgatc
      841 gcggtcgcac cgagcgcggc cagcacgacg agcatcgcgg cgaacggcca cgcatgcgtg
      901 aagagcgcca ccacgcagga gaacgccgcc atcagcgcgc cgagcagcgc cagcgtcatg
      961 tgcggtgaca gccagcggtc ggccagcgca ccccacgcga tgcgcgccgc cacgccggtc
     1021 aggctcgcgg tcgtcaggcc cacgccggct gcgaccagcg gcacgccgag ttcgtcggcg
     1081 aggtaggcga cgatgaagcc ggtcgtgcac atctgcatgc cggagtagac gaacgacacc
     1141 gcagccaccg tgcgcaagcg cggtgtcgtt atgaccagcc gcaatcccgc cgccagttgc
     1201 gccagcgata ttcgcgcgcg ccggtcacga tccgcatcga gttcggaccg cagcggctgc
     1261 gccgcaaccg caaccgcggc acacagcgcg gcgacgatca gcaccgcgat gcgccagccg
     1321 agcgccaccg ccagcggcgg cacgacgata cctgccagcg ccatcccggc cggcacaccg
     1381 gtctgcttga tcgacagcgt cagcgccatg ttgcgcggcg tggccgtgcg cgcgagcacg
     1441 tggctggagg ccggcgtgat cggcccgtag ccaagcccga gcagcacggc cgcggcagca
     1501 acgaatgcca gcgacggcac cagcgcgagc g
//
LOCUS       DBNHBY010000128         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1495783, whole genome shotgun sequence.
ACCESSION   DBNHBY010000128 DBNHBY010000000
VERSION     DBNHBY010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1495783"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /gene="soeB"
                     /locus_tag="AC16HB_03100"
     CDS             complement(<1..636)
                     /gene="soeB"
                     /locus_tag="AC16HB_03100"
                     /EC_number="1.8.5.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase subunit SoeB"
                     /protein_id="HIR5605807.1"
                     /translation="MTQLALVIDLNVCVGCHACVTSCKEWNTSGSAGTMADLRPYDAD
                     PTGTFFNRVQTFEVGEYPNTQTVHFPKSCLHCEEPPCVPVCPTGASHKRPEDGIVLVD
                     YDKCIGCKYCSWACPYGVRELDEKQQVMKKCTLCVDRIYDAKLPAFERKPACVMACPT
                     SARLFGDVHDPDSEVSIAIRERAGYALMPEWGTRPANHYLPRRRTELPIAAE"
     gene            complement(633..>2009)
                     /locus_tag="AC16HB_03105"
     CDS             complement(633..>2009)
                     /locus_tag="AC16HB_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR5605808.1"
                     /translation="AWRGDPYRIDTLLIFMANMAWNSTMNTVEVRRMLNDKDDNGDYK
                     IPFLVVCDAFQSETTAFADLVLPDTTYLERHDVMSILDRPISEFDGPVDSVRIPVVPP
                     TGECKPFQEVLIELASRLKLPAFTRPTGERRFRDYPDFVVNFETEPGSGIGFLSGWRG
                     RAGEKFMHGEPNPQQWERYKQNNCVFHYKLPPSYQYMRNWNKGYLEWSTRNRITRYDE
                     PILIHLYSEVLQRFRTAARGKGLTRKPPAHLRDRIATYFDPLPFYYAPLEVQATDTER
                     FPLAAITQRPMAMYHSWDSQNAWLRQIHTHNYLFVNPRTARAAGIGDGDWIWVESQWG
                     KVRCMCRYSEAVEAGTVWTWNAIGKAAGAWALAPDANESRKGFLLNHLITEELPALAG
                     NGTISNSDPVTGQAGWYDVRVRIRKADPNEDKESSPQFAPMKSFPGMATARRLWLAYT
                     TRRDGGAR"
BASE COUNT          302 a          666 c          681 g          360 t
ORIGIN      
        1 ctccgcggcg atcggcagtt cggtcctgcg ccgcggcagg tagtgattgg ccggacgcgt
       61 gccccattcg ggcatcagcg cgtagccggc gcgctcgcgg atcgcgatcg acacctccga
      121 gtccggatcg tgcacgtcgc cgaacaggcg cgcactggtc gggcacgcca tcacgcaggc
      181 gggcttgcgc tcgaaggccg gcagcttcgc gtcgtagatg cggtcgacgc acagcgtgca
      241 cttcttcatc acctgctgct tctcgtcgag ttcgcgcacg ccgtacgggc acgcccacga
      301 gcagtacttg cagccgatgc acttgtcgta gtcgaccagc acgatgccgt cctcgggccg
      361 cttgtggctg gcccccgtgg ggcacaccgg cacgcacggc ggctcctcgc agtgcaggca
      421 cgacttcggg aagtgcaccg tctgcgtgtt cgggtactcg ccgacctcga acgtctgcac
      481 gcggttgaag aaggtgccgg tcggatcggc atcgtacggc ctcaggtcgg ccatcgtccc
      541 ggcggagccg gacgtgttcc actccttgca gctcgtcacg cacgcgtggc agccgacgca
      601 cacgttcagg tcgatgacca aggccaactg ggtcaccgcg cccctccatc tcggcgtgtc
      661 gtatacgcga gccacagcct gcgcgcggtc gccatgccgg gaaacgactt catcggcgcg
      721 aactgcggcg acgattcctt gtcttcgttc gggtccgcct tgcggatgcg cacgcggacg
      781 tcgtaccagc cagcctggcc ggtgaccggg tcggagttcg agatcgtgcc gttgccagcc
      841 agcgccggga gctcctcggt gatcaggtgg ttcagcagga agcccttgcg cgactcgttg
      901 gcgtcggggg cgagcgccca cgcgccggcc gccttgccga tcgcgttcca cgtccacacg
      961 gtgccggcct cgaccgcctc cgagtagcgg cacatgcagc gcaccttgcc ccattgcgac
     1021 tcgacccaga tccagtcgcc gtcaccgatg cccgcggcgc gtgccgtgcg cggattgacg
     1081 aacaggtagt tgtgcgtgtg gatctgccgc agccacgcgt tctgcgaatc ccacgagtgg
     1141 tacatcgcca tcggccgctg cgtgatcgcc gcgagtggaa agcgctccgt gtcggtcgcc
     1201 tgcacctcga gcggcgcgta gtagaacggc agcgggtcga agtaggtcgc gatgcggtcg
     1261 cgcaggtggg cgggcggctt gcgcgtgagc cccttgccgc gcgcggcggt gcggaaccgc
     1321 tgcagcactt ccgaatacag gtgaatcagg atcggttcgt cgtagcgcgt gatgcggttg
     1381 cgcgtcgacc actccaggta gcccttgttc cagttgcgca tgtactggta cgagggcggc
     1441 agtttgtagt ggaacacgca gttgttctgc ttgtagcgtt cccactgctg cgggttcggc
     1501 tcgccgtgca tgaacttctc gcccgccctg ccgcgccagc cggagaggaa gccgatgccc
     1561 gatcccggct cggtctcgaa gttgaccacg aagtccgggt agtcgcggaa cctgcgctcg
     1621 ccagtgggcc gcgtgaaggc cggcagcttc agccttgagg ccagctcgat gagcacttcc
     1681 tggaacggct tgcattcgcc ggtcggcggc acgaccggga tgcgcaccga gtcgaccggg
     1741 ccgtcgaact ccgagatcgg ccggtcgagg atcgacatga cgtcgtggcg ctcgagatac
     1801 gtggtgtccg gcagaacgag gtcggcgaac gcggtcgtct ccgactggaa cgcgtcgcag
     1861 acgacgagga acgggatctt gtagtcgccg ttgtcgtcct tgtcgttcag catccggcgg
     1921 acctcgaccg tgttcatcgt cgagttccac gccatgttgg ccatgaagat cagcagcgtg
     1981 tcgatgcggt acggatcgcc gcgccacgc
//
LOCUS       DBNHBY010000129         2639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1427666, whole genome shotgun sequence.
ACCESSION   DBNHBY010000129 DBNHBY010000000
VERSION     DBNHBY010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1427666"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="AC16HB_03110"
     CDS             complement(<1..1244)
                     /locus_tag="AC16HB_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605809.1"
                     /translation="MYDRTPILVGAGQYVGREAHAAAQMLSPIEIAATAAARALADTG
                     ARTALGSEIDTLAVVRLFEHSVHDGAMWPNPLGSSNNVPWSVARRLRATPRTAIYAEV
                     GGQSPQRLVNTTAERIHAGEVRLAVLTGAEAIATIREATRRGIELDWHEEASGQFEDR
                     WPGAPFVTPYEQRHGIALPIHVYALFEQVRRRQRGLSLEACRAEIAAMLAPFTAIAAA
                     HPFAQFPTVHDAEFLRTVSSQNYALCEPYTKWMVAQDAVNQGAAVVLTSVGVARELGI
                     AESRWVYLAGYGDVDDVTVLSRSHLASSRAQLLAVQTALDAAGVGIEAIGYRDFYSCF
                     PIAVSSIAEALGLPLDGSVPLTLTGGLPFFGGPGNNYSMHAIATMIERIRRDRDAHGL
                     VVANGGYLSKHSAAVYSGKLARS"
     gene            1601..2440
                     /locus_tag="AC16HB_03115"
     CDS             1601..2440
                     /locus_tag="AC16HB_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605810.1"
                     /translation="MTARILSAVSNDDRVMPVTRIVAACVVPFLVIAFLSLYLRPDLS
                     ATHFTWKIVPTFTAFWIGTGYIGGAWFFYSVTRARRWHEVALGFLPVTAFVWMMLVAT
                     ILHWDRFDIHHLPFQLWLVLYAVTPFVVPLLWLWNRRRDPHRIEPGAVVVPGPVRGAM
                     GVIGVVLVLTALWMFLFPQAAIAAWSWKLTPLTARVMGGWLALSGVGGLVLASEPRWS
                     AWRVLIQSMLVWLLLLTIGVFRSWDEFDLSRPSIVAVFAVPASLLGLGAVYLAFEHKR
                     PGG"
BASE COUNT          414 a          849 c          874 g          502 t
ORIGIN      
        1 gaccgcgcca gcttgccgct gtagaccgcg gccgaatgct tggacagata gccgccattg
       61 gcgacgacca gcccatgcgc atcgcgatcg cggcgaatgc gttcgatcat ggtcgcgatc
      121 gcatgcatgc tgtagttgtt gccggggccg ccgaagaatg gcaggccccc agtgagcgtc
      181 aaaggcaccg aaccgtcgag cggcaggccg agtgcctcgg ctatcgacga caccgcgatc
      241 ggaaaacagc tatagaaatc gcgataaccg atggcttcga taccgacgcc ggccgcgtcg
      301 agcgcggttt gcacggcgag cagctgagcc cgtgacgagg ccaggtgtga acgcgacagt
      361 acggtgacgt cgtcgacatc gccatagcct gcgagataga cccatcgcga ttccgcgatg
      421 ccgagttcgc gcgccacacc gaccgaggtc agcaccaccg ccgcgccctg gttgaccgcg
      481 tcctgcgcca ccatccactt cgtatagggc tcgcacagcg cgtagttctg cgacgacacc
      541 gtgcgcagga attcggcgtc atggaccgtc ggaaactgcg caaacggatg agctgccgca
      601 atcgcggtaa acggtgccaa catcgcggcg atctcggcgc ggcaggcctc gagtgacagc
      661 cctcgttgcc gccgtcgaac ctgttcgaac aatgcataga catggatcgg caaggcgatg
      721 ccgtggcgct gctcgtaggg cgtgacgaac ggcgcgccgg gccagcgatc ctcgaactga
      781 ccgcttgctt cttcgtgcca gtcgagttcg atgccgcgcc gcgtcgcctc gcggatggtg
      841 gcgatggcct cggcgccggt aagtacggcg agtcgcacct cgccggcatg aatgcgctcg
      901 gcggtggtgt tgaccaggcg ctgcggcgat tgtccgccga cttcggcata gatcgccgtg
      961 cgtggcgttg ctcgcagtcg ccgcgccacc gaccacggta cgttgttgga cgagccgagc
     1021 ggattcggcc acatcgcgcc atcgtgcacc gagtgctcga acaggcggac caccgccagc
     1081 gtgtcgatct cggagccgag cgccgtgcgc gcgccggtgt cggcaagtgc gcgcgcggcg
     1141 gcggtcgcgg cgatttcgat cggcgaaagc atctgcgcgg cggcatgcgc ctcgcggccg
     1201 acgtattggc cggcaccgac gagtatgggg gtacggtcgt acatgacgaa ggctgctcca
     1261 cgcgcgcagg accgttctgc ggcctgctac gacaggccgt gaagcgaatc gctcgagacc
     1321 gcaaaataac accggagatt tcttgcgcct gcagtgactc actgcgccgt actatcgatc
     1381 gtcacatggc tgccgagtcg aggcggccgg caagtgccat agtggcggtg gtcatcgtgc
     1441 ttcctttgga caagcgttgg tgggtcgcac ctcgaccttg tccggattca cgatggccgc
     1501 ccccaagtga cggtcgccgc cgcgcggatg gtcatccggt catctgtctc ggcccgccgt
     1561 cgcgccattc tgatcgatat gatcgtcgag gaggataccg atgactgctc gcatcttgtc
     1621 cgccgtatcc aacgacgatc gcgtgatgcc tgtgacgcgc atcgtggcgg cctgcgtcgt
     1681 gccgtttctc gtgatcgcgt tcctgtccct gtacctgcgg cccgatctca gcgcgacgca
     1741 cttcacctgg aagatcgtgc cgaccttcac tgccttctgg atcggcactg gctacatcgg
     1801 cggcgcatgg ttcttctaca gcgtcacgcg cgcccggcga tggcacgagg tagcgctcgg
     1861 ctttctaccg gtcacggctt tcgtctggat gatgctggtg gcgacgatcc tgcactggga
     1921 tcgcttcgac attcaccatc tgccgttcca actctggctc gttctctacg cggtgacgcc
     1981 gttcgtcgtg ccattgctgt ggctgtggaa ccgacgccgc gatccgcatc ggatcgaacc
     2041 gggcgcagtc gtcgtgcccg gcccggtgcg gggggcgatg ggcgtgatcg gcgtggtgct
     2101 cgtgctgacc gcgctgtgga tgttcctgtt cccgcaggcg gccatcgccg cgtggtcgtg
     2161 gaagctcacg ccgctcaccg cacgcgtgat gggcggatgg ctcgcgctgt cgggcgtggg
     2221 tggactggtg ctcgcgagcg agccacgctg gtcggcctgg cgcgtcctga tccagagcat
     2281 gctggtgtgg ctgctgctgc tcacgatcgg cgtgttccgt tcctgggacg agttcgactt
     2341 gtcgcgcccg agcatcgtgg ccgtgttcgc agtgcccgcg tcccttctgg gtctcggtgc
     2401 ggtctatctg gcgttcgagc acaagcggcc cggcggctga tcgaaaagcg attgccttcg
     2461 cccgccgcgc tctcatttct tcggacgctg cttcgcgaag gcgttgggca cgctgcgcca
     2521 cggcaaaaca acagtctgac ggcaaccatg tgatcgttgc tgcagccgag tgtctgctct
     2581 tgcagcggtg gaaatgtccg catcgcacgc cccagtgaat gaccgctatc catgaggtg
//
LOCUS       DBNHBY010000130         2629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_412666, whole genome shotgun sequence.
ACCESSION   DBNHBY010000130 DBNHBY010000000
VERSION     DBNHBY010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_412666"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="AC16HB_03120"
     CDS             <1..697
                     /locus_tag="AC16HB_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5605811.1"
                     /translation="PYASLNPRMRAADIVVEPLRNFEPMSDSAARERAASLFARVGLR
                     ADQLVKYPFEFSGGQRQRLGIARALSVQPQLIVCDEPVSALDVSVQAQVINLLMDLQR
                     EFHLSYLFVAHDLAVVEHISHRVAVMYLGKIVEIADKKSIFTRPQHPYTEALLEAVPV
                     PDPKRRKQRRVLTGDVPSPVNPPPGCRFHTRCPYVEARCKVEEPPLKQVAAGQWVACH
                     LRQPVAAKEEAVA"
     gene            694..1911
                     /locus_tag="AC16HB_03125"
     CDS             694..1911
                     /locus_tag="AC16HB_03125"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR5605812.1"
                     /translation="MTREARAIRNPAAAPAHEPYGAQRAQVLSQAGFDAALAEVSSWP
                     GYAATPLAPLNGLAKTLGVGSIHYKDERGRFSRYGLKSFKALGGAYAVANVLRQRVAA
                     ATGRADITSRQLLSGEFAAIVRAATVTCATDGNHGRSVAWGAQLFGCNCVIYVHEHVS
                     QGRRDAIAKYGADVREVKGNYDDAVRHAAATAQANGWTVVSDTSYPGYRDIPLDVMHG
                     YGVMAAEIERQLDAPPTHVFAQAGVGALAAAMCAHFWLHWKEQRPFFVVVEPIRADCV
                     YASLAAGKPVVVGGSLDTVMAGLACGEVSELAWEILHGGANAAVAVDDDYALDAVRLL
                     ARPTAGDAPIVAGETGGSGLAALLAARDYPEIRNTLALDGNARVLLLGSEGDTDPQIY
                     REVVGRSAEEVLA"
     gene            1908..>2629
                     /locus_tag="AC16HB_03130"
     CDS             1908..>2629
                     /locus_tag="AC16HB_03130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HIR5605813.1"
                     /translation="MKAESLRINPARLLARLNELAAIGATPDGSCCRLALTDEDKAGR
                     DLVVRWMRELGLEVRIDQAGNIFGLQRGAEDVAPVMTGSHIDTVRTGGRYDGCLGVLG
                     GLEVVQTLKDAGLTTRRPLVVGAFTDEEGARFHPDMAGSLVYVGGMPLAELYATRGID
                     GKLFRDELERIGYLDDAPLPLYKPHAYVELHIEQGPVLDKEGITIGAVEDLQGISWTE
                     VLITGQSNHAGTTPMRMRHDAG"
BASE COUNT          412 a          868 c          962 g          387 t
ORIGIN      
        1 cccctatgcg tcgctgaacc cgcgcatgcg agccgccgac atcgtggtcg agccgctgcg
       61 caacttcgag ccgatgtcgg acagcgctgc gcgcgagcgc gcggcgagcc tgttcgcacg
      121 ggtcggcctg cgcgccgacc agttggtcaa gtatccgttc gagttctcgg gcgggcagcg
      181 gcagcggctc ggcatcgcga gagcgctttc agtgcagccg cagctgatcg tctgcgacga
      241 gccggtgtct gcgctcgacg tgtcggtgca ggcgcaggtg atcaacctgc tgatggattt
      301 gcagcgcgag ttccatctgt cgtacttgtt cgtggcgcat gacctggccg tggtcgagca
      361 catcagccat cgcgtcgcgg tgatgtacct cggcaagatc gtcgagattg ccgacaagaa
      421 atcgatcttc acgcggccgc agcacccgta caccgaggcg ctgctggaag cggtgcctgt
      481 gcccgacccc aagcggcgca agcagcggcg cgtgctgacc ggcgacgtgc cgagccccgt
      541 caatccgccg ccgggctgcc gcttccacac gcgctgcccg tacgtggagg cgcgctgcaa
      601 ggtcgaggag ccgccgctca agcaggttgc ggccggacag tgggtcgcgt gtcacctgcg
      661 gcagccggtt gctgcgaaag aggaggccgt cgcatgacgc gcgaggcacg cgccatccgc
      721 aatcccgccg cggcgccggc gcacgagccg tacggcgccc agcgcgcgca ggtgctgagc
      781 caggcgggct tcgacgccgc gctggcggaa gtatcgagct ggcccggcta tgcggccacc
      841 ccgctcgcgc cattgaacgg gctggcgaag acgctgggcg tcggcagcat tcactacaag
      901 gacgaacgcg ggcgcttctc gcgttacggg ctgaagagct tcaaggcgct cggcggcgcg
      961 tacgccgtgg ccaacgtgct gcgccagcgc gtggcggcgg cgacggggcg cgcggacatc
     1021 acgtcgaggc aactgctgtc gggcgagttc gccgccatcg tgcgcgccgc aacggtcacg
     1081 tgcgcgaccg acggcaatca cggccgctcg gtcgcctggg gcgcgcaact cttcggctgc
     1141 aactgcgtga tctatgtgca cgagcacgtg agccaggggc ggcgcgacgc gatcgcgaag
     1201 tacggggccg acgtgcgcga ggtgaaagga aactacgacg atgccgtgcg ccatgcggcc
     1261 gcgaccgcgc aggccaacgg ctggacggtg gtgtcggata cgtcgtatcc cggctaccgc
     1321 gacattccgc tcgacgtgat gcacggctac ggcgtgatgg cggccgagat cgaacggcaa
     1381 ctcgacgcgc cgccgacgca cgtgttcgcg caggcgggcg tgggcgcgct cgcggcggcg
     1441 atgtgcgcgc acttctggct gcactggaag gagcagcggc cgttcttcgt cgtggtggag
     1501 ccgattcgcg ccgactgcgt gtacgcgagc cttgccgcgg gcaaaccggt cgtcgtgggc
     1561 ggctcgctcg atacggtgat ggccgggctc gcgtgcggcg aagtgtcgga actcgcgtgg
     1621 gagatcctgc acggcggcgc caatgccgcg gtcgcggtcg acgacgacta cgcgctcgac
     1681 gcggtgcgcc tgctcgcgcg gccgaccgcg ggtgacgcgc cgatcgtcgc tggcgaaacc
     1741 ggggggtcgg ggctggctgc gctgctcgcc gcgcgcgact atcctgagat tcgcaacacc
     1801 ctggcgctcg atggcaacgc gcgcgtgctg ctgctcggca gcgaaggcga caccgacccg
     1861 cagatctatc gcgaggtggt cgggcgcagc gcagaggagg tactggcatg aaagcggaaa
     1921 gcctgagaat caatccggcg cgcctgctgg cgcggctgaa tgaactcgcg gcgatcggcg
     1981 cgacccccga cggcagttgc tgccggctcg cgctgaccga cgaggacaag gcggggcgcg
     2041 atctcgtcgt gcggtggatg cgtgagcttg gcctcgaagt gcgtatcgac caggccggca
     2101 acatcttcgg cctgcagcgc ggcgccgagg atgtggcgcc ggtgatgacc ggatcgcaca
     2161 tcgacaccgt gcgcaccggc ggacgctacg acggctgcct cggcgtgctc ggcgggctcg
     2221 aagtcgtgca gacactcaaa gacgccggcc tcaccacgcg ccggccgctg gtggtcggtg
     2281 cgttcaccga cgaggaaggc gcgcgcttcc atcccgacat ggccggctcg ctcgtctacg
     2341 tcggcggcat gccgctcgcg gagttgtacg cgacgcgcgg catcgacggg aaactcttcc
     2401 gcgacgagtt ggagcgcatc ggctacctcg acgatgcgcc gctgccgctg tacaagccgc
     2461 atgcgtacgt cgagttgcac atcgagcagg ggccggtgct cgacaaggaa ggcatcacga
     2521 tcggcgcagt cgaggacctg cagggcatct cctggaccga ggtgctgatc accggccagt
     2581 cgaaccacgc cggaacgacg ccgatgcgga tgcggcacga cgccggcta
//
LOCUS       DBNHBY010000131         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1292923, whole genome shotgun sequence.
ACCESSION   DBNHBY010000131 DBNHBY010000000
VERSION     DBNHBY010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1292923"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16HB_03135"
     CDS             complement(<1..413)
                     /locus_tag="AC16HB_03135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR5605814.1"
                     /translation="MAIVIDALVQLAQGVIVLLLAPLLTGYVRKVKARLLRRQGPPLI
                     QPYRDLVKLLHKEVVLAHNASWVFRVAPYIIFATTWVAAALVPTFATGLALRPAADLI
                     AIVALLGSARFMLALAGLDVGTSFGGIGSSREMMIA"
     gene            complement(404..>1930)
                     /gene="hyfB"
                     /locus_tag="AC16HB_03140"
     CDS             complement(404..>1930)
                     /gene="hyfB"
                     /locus_tag="AC16HB_03140"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR5605815.1"
                     /translation="GYIYLLMASLGTLSLLLAFGLLAGPGSGYGFESIRTSELSPAVA
                     AAVLALVLLGAGSKAGLVPMHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDL
                     MGPPAWWWGAVVLLFGGVTAVLGVLHALMEHDLKRLLAYHTVENIGIIFIGLGLALAF
                     SANQIWAPAALAVTAALFHVFNHSIFKSLLFFGAGAVLTATGARDMERLGGLIHAMPV
                     TAFAFLIGSAAISALPPFNGFVSEWLTFQAILLSPQLPQWLLKFLVPAVGAMLALSAA
                     LAAACFVKAFGITFLGRPRSDVAQRAREVDAFSLTAMLTFVLLCLVAGILPGYFIDAL
                     APVAQTAVGAHMPQQQALEWLVIVPVAESRSSYNGLALFLLIALAASLAAYVIHRWAS
                     HAVRRSAPWDCGFPDPDPRAQYTAGSFAQPIRRVFGSFVFRAREQVQMPPPGDPSPAR
                     LEVHLRDLIWEFAYAPIERVVGYTADLFNRVQFLTIRGYLTLVSGALVALLVVLAVWQ
                     "
BASE COUNT          391 a          649 c          659 g          231 t
ORIGIN      
        1 gcgatcatca tttcgcggct cgacccgatg ccgccgaagc tggttccgac atccagcccc
       61 gccagcgcga gcatgaaacg cgcgcttccc agcagcgcaa cgatcgcgat caggtcggcg
      121 gccggacgga gcgcgagtcc cgtggcgaag gtcggcacga gcgccgcggc gacccaggtc
      181 gtcgcgaaga tgatgtaagg cgccacgcga aagacccacg aggcgttgtg cgccagcacg
      241 acctccttgt gcagcagctt gaccaggtcg cgatacggct gaatcagcgg cgggccctga
      301 cggcgcagca gacgcgcctt caccttgcgc acgtacccgg tcagcagcgg ggcgagcagc
      361 aggacgatca cgccctgcgc gagctggacg agcgcatcga tgactattgc catacggcca
      421 gcaccaccag gagcgcgacc agcgcgccgg aaaccaatgt cagatagccg cggatggtca
      481 ggaactgcac ccgattgaac agatcggccg tgtagccgac gactctctcg atcggcgcat
      541 acgcgaactc ccagatgaga tcgcgcagat gcacttccag ccgcgccggg ctcgggtcgc
      601 cggggggcgg catctgcacc tgctcgcggg cacgaaacac gaagctgccg aacacgcggc
      661 ggatcggctg cgcgaaactg cccgcggtgt actgcgcgcg cgggtccgga tcgggaaagc
      721 cgcagtccca cggcgccgag cgccggaccg cgtgcgacgc ccagcggtga atcacataag
      781 ctgcaagact ggcggccagg gcgatcagca ggaacagtgc gagcccgttg taggagctgc
      841 gactctcggc gacgggaacg atcaccagcc actcgagcgc ctgctgctgc ggcatgtgcg
      901 caccgaccgc ggtctgcgcc accggagcga gcgcatcgat gaagtagccc ggaagaatgc
      961 cggcgacgag acacagcagc acgaacgtca gcatcgccgt cagcgagaag gcatccactt
     1021 cgcgggcacg ctgcgcgaca tcggatcgtg gccgtcccag aaaggtgatg ccgaatgcct
     1081 tgacgaagca ggccgccgcc agcgcggcgg acaaggcgag catcgcaccg accgcgggca
     1141 cgagaaactt cagcagccac tgcggcagtt gcgggctgag caggatcgcc tggaacgtga
     1201 gccactcgga gacgaagccg ttgaacgggg gcagcgccga gatcgcggcc gaaccgatca
     1261 ggaaggcaaa agccgtcacc ggcatcgcat ggatcagtcc gccgagccgc tccatgtcgc
     1321 gtgcaccggt cgccgtcagg acggcgccgg cgccgaagaa cagcaggctc ttgaagatcg
     1381 agtgattgaa cacgtggaac agcgccgcgg tgacggccag ggctgccggc gcccagatct
     1441 ggtttgcgct gaaggcgagc gcgagaccga ggccgatgaa gatgatgccg atgttctcga
     1501 ccgtgtgata cgcgagcagg cgcttcagat cgtgctccat cagcgcgtgc agaacgccga
     1561 gcaccgcggt cacgccgccg aagagcagca cgacggctcc ccaccaccac gccggcggcc
     1621 ccatcaggtc gaagacgatg cgcacgaagc cgtacaccgc gaccttggtc atcacgccgc
     1681 tcatcagcgc cgagacgtgg ctcggcgccg ccgggtgcgc cagcggcagc cagacgtgca
     1741 tcggcaccag tccggctttc gagcctgcgc ccagcagcac gagcgcgagg acggcggcgg
     1801 cgacggcggg cgacagctcg ctcgtgcgga tcgactcgaa cccgtagccg ctgcccgggc
     1861 cggccagcag accgaaggcg agcagcagcg acagcgtgcc gaggcttgcc atcagcaggt
     1921 agatgtatcc
//
LOCUS       DBNHBY010000132         1991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1495963, whole genome shotgun sequence.
ACCESSION   DBNHBY010000132 DBNHBY010000000
VERSION     DBNHBY010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1495963"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16HB_03145"
     CDS             complement(<1..194)
                     /locus_tag="AC16HB_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5605816.1"
                     /translation="MSLLWTILVGFVVGLIAKLVHPGRENMGIIMTTLLGIGGSLLAG
                     YVGQAIGWYQAGQGAGFIGSL"
     gene            382..1662
                     /gene="hemA"
                     /locus_tag="AC16HB_03150"
     CDS             382..1662
                     /gene="hemA"
                     /locus_tag="AC16HB_03150"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR5605817.1"
                     /translation="MQLLTLGINHQTAPLALRERVAFVPEEIGPTIARLRDRLALRTA
                     GALSEAAIVSTCNRTELYCAVAEPQAASTALAEFIAVEKQLDTDEVRRSAYALPQTNA
                     VRHAFRVASGLDSMVLGEPQILGQMKQAERLARDAGGMGLMLNHLFQHTFAVAKEVRT
                     TTEIGAHSVSMAAAAVRVAERIFGSLVDSDVLFVGAGEMIELTGTHFAARHPKSITVA
                     NRTIERGDTLAAKLQGRSMRLAELPEAIARFDIVVSCTASSLPIIGLGMVERAIKTRR
                     HRPMFIVDLAVPRDVEPEVARLDDVYVYTVDDLGKIVQSGTESRQAAVAQAEAIIESR
                     VRDFDAWLRTRAAVPIIQGLRSRADELRQYELDRARRLLARGESPEAVLEQLSQALTN
                     KFLHAPVAALHQASSHDNDEERARLVALLSRFYS"
     gene            1729..>1991
                     /locus_tag="AC16HB_03155"
     CDS             1729..>1991
                     /locus_tag="AC16HB_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR5605818.1"
                     /translation="MQAKLAHLARRLNEIDTALASEDATRDMNRYRALGQERAEIEPV
                     VARFHDYNRAQADLAGAQEMLAEPDMKALAEEEIHVARGRIAAL"
BASE COUNT          344 a          670 c          679 g          298 t
ORIGIN      
        1 agcgaaccga tgaatccagc gccctgccct gcctggtacc agccgatcgc ctgtccgaca
       61 tacccggcca gcagcgagcc gccgatgccg agcagcgtcg tcatgatgat ccccatgttt
      121 tccctgccgg ggtgcacgag cttggcgatc aaaccgacga cgaaccccac cagaatggtc
      181 caaagcagtg acattgtctc ctccttgacc tgtgacaagc cgcccgattc ggaacgaccc
      241 cgatcggccg cggctgcgac agaatcctcc cgcaggtgga aattgtcacc cccaccgtaa
      301 cgcgggaggc gggccggcat ttcggctgcc gcgtaagttg gagccttacg gcctaaaatt
      361 caaggccttg ccggaacgac tatgcagttg ctgacactcg ggatcaacca ccagaccgcg
      421 ccgcttgcgc tgcgcgagcg ggtggcgttc gtcccggagg aaatcggccc gacgatcgcg
      481 cggctgcgcg accggctggc gctgagaacc gcaggcgccc tttccgaagc ggcgatcgtg
      541 tcgacctgca accgcacgga actctactgc gcggtcgccg agccgcaggc ggcgagcacg
      601 gcgctggcgg agttcatcgc ggtggaaaag caactcgaca ccgacgaggt gcggcgttcc
      661 gcctacgcgc tgccgcagac caacgcggtg cggcacgcgt tccgggtggc gagcgggctg
      721 gattcgatgg tgctcggcga gccgcagatc ctgggccaga tgaaacaggc cgaacgcctg
      781 gcgcgcgacg ccggcggcat gggcctgatg ctgaaccatc tgttccagca cacgttcgcg
      841 gtcgccaagg aagtgcggac cacgacggag atcggcgccc attcggtgtc gatggccgcg
      901 gctgcggtgc gcgtggccga gcgcatcttc ggctcgctcg tcgatagcga cgttctgttc
      961 gtcggtgccg gcgagatgat cgaactgacc ggtacgcact tcgcggcgcg ccatccgaag
     1021 tcgatcacgg tggccaaccg cacgatcgag cgcggggaca cgctcgccgc caagctgcag
     1081 ggccgctcca tgcggctggc cgagctgccc gaggcgatcg cgcgtttcga catcgtggtg
     1141 tcgtgcacgg cgagttcgct gccgatcatc ggcctgggca tggtcgagcg cgcgatcaag
     1201 acgcggcggc accggccgat gttcatcgtc gatctggcgg tgccgcgcga cgtggagccc
     1261 gaggtggcgc ggctggacga cgtgtacgtc tacacggtcg acgacctggg caagatcgtg
     1321 cagagcggca ctgaaagccg gcaggccgcc gtcgcgcagg ccgaggcgat catcgagtcg
     1381 cgcgtgcgcg atttcgacgc ttggctgcgc acccgcgccg cggtgccgat catccaggga
     1441 ctccgaagcc gcgccgacga gctgcgccag tacgagctgg atcgcgcccg cagactgctg
     1501 gcgcgcggtg aatcgcccga ggctgtgctc gagcagcttt cgcaggcgct gaccaacaag
     1561 ttcctgcacg cgccggtggc cgcgttgcat caggcgtcca gccacgacaa cgacgaagag
     1621 cgcgcgcgcc tcgtggcgct gctctcccgc ttttactcat agggtcgggg atcgaggcgg
     1681 ccgccggccg cgtcgcactc cttttgttca aagccgatga agccttcgat gcaagccaag
     1741 ctcgcgcacc tggcgcggcg actgaatgaa atcgataccg cgctggcgag cgaggatgcg
     1801 acgcgcgaca tgaaccgcta ccgcgcgctc gggcaggagc gcgccgagat cgagcccgtc
     1861 gtggcgcgct tccatgacta caaccgcgcg caggccgacc tcgccggcgc acaggagatg
     1921 ctggccgagc cggacatgaa agcgctggcc gaggaagaga tccacgtcgc ccggggccgg
     1981 atcgccgcgc t
//
LOCUS       DBNHBY010000133         3756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_683898, whole genome shotgun sequence.
ACCESSION   DBNHBY010000133 DBNHBY010000000
VERSION     DBNHBY010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_683898"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC16HB_03160"
     CDS             <1..434
                     /locus_tag="AC16HB_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5605819.1"
                     /translation="SRIGAVLAGADAVTEEHAAQYGSSLGTAFQMIDDLLDYSGNISE
                     IGKRLGDDLREGKVTLPLIHALRLAHPREREKIERAIREGGGDFDQIATIVRGNGSLD
                     YVRERALTESTAAESALAAFPASAYRDSLVELNAFAMRRNH"
     gene            489..565
                     /locus_tag="AC16HB_03165"
     tRNA            489..565
                     /locus_tag="AC16HB_03165"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:523..525,aa:Pro,seq:cgg)
     gene            612..1880
                     /locus_tag="AC16HB_03170"
     CDS             612..1880
                     /locus_tag="AC16HB_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR5605820.1"
                     /translation="MEGIPLWAQVTALVILLILAAFFSLAETAMMATSRLRLRHLVRQ
                     GSRAARLTQSLLERTDQLLGVILLGNTLLNAGATALVTALAIYYFGDNQWSLVLATGS
                     ISFLILVFAELTPKVIGATYPEHIALPSSYAIAPLLRLMAPAIWFVNLFVRVLLRLAR
                     VPQNAGAETQRLTPEELRTIVLEGGGVVRGKHRSILLNLFDLEQVTVDDLMTPRARIE
                     LLDVSHPVAAIRRQLATCYHNKLPVVDGDITRVIGVMHIRKLLTLLDADSLSHESIRE
                     ILVAPYFVPTGTPILQQLQMFQDNQQRLGLVVDEYGEVQGLITLEDIVEEIVGEFTTQ
                     APGLGTRGLRWDDHGHAVVDGTTPLRELNRRLSLRFSLAGPKTVNGLILEALQEIPSG
                     PCCIRYDRCIVEVIQIHDQSVRSVRLVRAA"
     gene            2067..>3756
                     /gene="pilB"
                     /locus_tag="AC16HB_03175"
     CDS             2067..>3756
                     /gene="pilB"
                     /locus_tag="AC16HB_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIR5605821.1"
                     /translation="MATSSLTGLARALVQQGRLRPDRAETLARKAAQANAQFIDELVG
                     ASETTGLSAAQIARFAADTFGHPLLDIAAFDQDQMPRDIIDRKLVGSLRVLALRKRGN
                     RLAVAVSDPTNFQALDQVKFQTQLALDVVVVEHDKLLRLIESSSQTVAQSLDKLVDDE
                     INFDELLSDAPDTTQEEATNAEVDDAPVVRFLQKLLLDAITDGASDLHFEPYEKFYRI
                     RMRVDGVLREIAQPPLAIKERLVTRIKVISRLDISEKRVPQDGRMKLALSGQRAIDFR
                     VSTLPTLFGEKVVMRILDPSQAKLGIDALGYEPEQKQLLVDAIQRPYGMVLVTGPTGS
                     GKTVSLYTCLNILNVPGVNISTAEDPAEIQLAGINQVNVNEKAGLTFANSMRAFLRQD
                     PDVIMVGEIRDLETADIAVKAAQTGHMVMSTVHTNDAPSTLTRLSNMGVAPFNIASSV
                     ILITAQRLARRLCSCKQPTEISKDALLGAGFDGADVDGSWVPYKPVGCERCKGTGYKG
                     RTGIFEVMPITDEIQRLILKNSSSVEIAAQAKREGVKNLRQSGLLKVKQGITSVD"
BASE COUNT          704 a         1192 c         1181 g          679 t
ORIGIN      
        1 cgagccggat cggcgctgtc cttgcggggg cggatgccgt gaccgaggag catgcggcgc
       61 agtatggctc gagcctcggg accgccttcc agatgatcga tgatctgctc gattattccg
      121 gcaacatcag cgagatcgga aagcggctcg gtgatgatct gcgcgagggc aaggtcacac
      181 tgccgctgat ccatgcattg cggctggcac atccgcgcga gcgcgagaag atcgaacggg
      241 caattcgcga aggcggcgga gacttcgatc agatcgccac catcgtgcgc ggcaacggct
      301 cgctcgacta cgtccgcgag cgcgcgctga ccgaatcgac cgccgcggag tccgctctcg
      361 ccgcctttcc cgcctcggcc tatcgagact ctttggtaga attgaatgcg tttgcgatgc
      421 gacggaacca ctgatttcgt tgctgcaagg cagttcagcc ggcgagccgc ctggcactga
      481 actggcgtcg gggtgtagct cagcctggta gagtactgcg ttcgggacgc aggagtcgga
      541 ggttcaaatc ctctcacccc gaccatcgtt ttccttcgcg ccggatgatt cagtccgcct
      601 ggaactgacg attggaaggc atcccgctct gggcgcaggt cacggcgctg gtcatcctgc
      661 tgatcctcgc cgcgttcttc tcgctggcgg aaacggcgat gatggccacc agccgtctgc
      721 ggcttcgcca tctggtgcgc cagggcagcc gcgccgccag actgacccaa tcgctgctcg
      781 agcgcactga tcagctactc ggcgtcatcc tgctcggcaa tacgctcctg aacgccggcg
      841 ccaccgcgct ggttacagcg ctggcgatct attacttcgg cgacaaccag tggtcgttgg
      901 ttctggcgac aggctccatc tcgttcctga tccttgtgtt cgccgaattg acgcccaagg
      961 tcatcggcgc gacctatcca gagcacatcg cgctgccctc cagttacgcg atcgcgccac
     1021 tactgcgcct gatggcgccg gcgatctggt tcgtgaatct cttcgttcgc gtcctgctgc
     1081 ggctcgcgcg cgtgcctcag aacgccgggg ccgaaacgca gcgactgacg ccggaagaac
     1141 tgcgcaccat cgtgctcgaa ggtgggggcg tggttcgcgg caagcatcgc agcatcctgc
     1201 tgaacctgtt cgacctcgag caggtcacgg ttgatgatct gatgacaccg cgcgcgcgca
     1261 tcgaactgct cgacgtttcg catccggtgg ctgcgattcg tcggcaactg gccacctgct
     1321 accacaacaa gctgccggtg gtcgacggtg acatcacgcg cgtgatcggc gtgatgcaca
     1381 tcagaaagct gctaacgctg ctcgacgcgg attcgctttc gcacgaaagc atccgggaga
     1441 tcctcgtggc cccgtacttc gtgccgaccg gcacgccgat cctgcagcag ttgcagatgt
     1501 tccaggacaa ccagcagcgc ctcgggctcg tcgtggacga atacggcgaa gtgcagggtc
     1561 tgatcactct cgaggatatc gtcgaggaga tcgtcggcga attcacgacg caggcgccgg
     1621 ggctggggac acgcggactg cgatgggacg atcacggcca cgctgtggtc gacggcacca
     1681 cgccattgcg cgaactcaat cgacgcctgt cgctgcggtt ctcgttggcg ggacccaaga
     1741 ccgtgaacgg gctgatcctt gaagcactgc aggaaattcc gagcggcccc tgctgcatcc
     1801 ggtacgatcg ctgcatagtc gaagtcatcc agattcatga ccaatcggtc cggagcgtgc
     1861 gcctggtccg agcggcgtga atgtgcaatc tccgatcgat cggttacgcc gtttgcccca
     1921 tcgcctaata cgttggtttg ctttacaaaa cgagggcttg tcggcagtat cgtgagcctc
     1981 gactcgaact ttgtgcggcg cgcagaccgg ttcgaccgac ccgcctctcc gaggtccaga
     2041 tgtcagctgt aagtcagagc accgcgatgg caacgtcctc cctgacggga ttggcgcgcg
     2101 cgctcgtgca gcaagggagg cttcgtccgg atcgcgccga gacgctcgcc cgcaaggcgg
     2161 cccaggcaaa cgcccagttc atcgacgaac tggtcggagc gagcgaaacg accggcctgt
     2221 cagccgcgca gatcgcgcgc ttcgccgccg acaccttcgg gcacccgctg ctcgacatcg
     2281 ccgcgttcga tcaggaccag atgccgcgcg acatcatcga tcggaagctg gtcgggtcgc
     2341 tgcgcgtgct ggcgctgcgc aagcgcggca atcgactcgc cgtggctgtg tcggatccga
     2401 ccaatttcca ggcgctggac caggtcaagt tccagaccca gcttgcgctc gatgtcgtgg
     2461 tggtcgagca cgacaagctg ctcaggctga tcgaatcctc cagccagacg gtcgcgcagt
     2521 ccctggataa actggtcgac gacgagatca acttcgacga actgctgtcg gatgcgccgg
     2581 acacgacgca ggaggaagcg accaatgccg aggtcgatga cgcgccggtc gtccgcttcc
     2641 tgcagaagct gctgctcgac gccatcaccg acggcgcctc cgacctgcac ttcgagccgt
     2701 acgagaagtt ctaccgtatc cgcatgcgcg tcgacggcgt gctgcgggag atcgcgcagc
     2761 caccgctcgc gatcaaggag cgcctggtca cgcggatcaa ggtcatctcc aggctcgaca
     2821 tctccgagaa gcgcgtgccg caggacggcc gcatgaagct cgcgctgtcg ggacagcggg
     2881 cgatcgactt ccgcgtcagc actctgccca ccctgttcgg cgaaaaggtc gtgatgcgga
     2941 ttctggatcc ctcgcaggcc aagctgggga tcgacgcgct cggttacgag ccggaacaga
     3001 agcaactgct cgtcgatgca attcagcgtc cgtacggcat ggtgctggtc acgggaccga
     3061 ccggcagcgg caagacggta tcgctctata cgtgcctgaa catcttgaat gtgccgggcg
     3121 tcaacatctc cacagcggaa gacccggcgg aaatccagct tgcgggcatt aaccaggtca
     3181 acgtcaacga gaaagccgga ctgacgttcg cgaactcgat gcgagcgttt ctgcggcagg
     3241 acccggacgt gatcatggtc ggcgagattc gcgacctcga gacggccgac atcgccgtga
     3301 aggcggcgca gaccggccac atggtcatgt cgacggtgca caccaacgat gcgccgtcga
     3361 ctctgacgcg actgtcgaac atgggcgtag caccgttcaa cattgcctct tccgtgattc
     3421 tgatcaccgc acaacgcctg gcacgacgac tgtgctcctg caagcagccg accgagattt
     3481 cgaaagacgc gctgctcgga gcgggcttcg acggggccga cgtcgacggt agctgggtcc
     3541 cctacaagcc ggtcggctgc gagcgctgca agggcactgg ctacaagggt cgcaccggaa
     3601 tcttcgaggt catgccgatc acggacgaga tccagcgcct gatcctgaag aattcaagct
     3661 cggtcgagat tgccgcgcag gccaagcgcg aaggcgtgaa gaatctgagg cagtcgggct
     3721 tgctgaaggt caagcagggc atcacctcgg ttgacg
//
LOCUS       DBNHBY010000134         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_142533, whole genome shotgun sequence.
ACCESSION   DBNHBY010000134 DBNHBY010000000
VERSION     DBNHBY010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_142533"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC16HB_03180"
     CDS             <1..572
                     /locus_tag="AC16HB_03180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5605822.1"
                     /translation="TIADPQVRYKGTIGGDISHGDPGNDHPALMIALDASFVSSGPKG
                     ERVVKADGYFLGLYSTLLEPNEILTQIRIPIPAAGTGWSYQKLKRKAGDFATAATAVL
                     LQMKGGSIAKVAIALTNVGPTALKASAAEAYLSGKTINEASLNEAARLAMSICDPAPD
                     QRGDAAYKTAMCGEMTRRALQAAAARAKG"
     gene            588..1082
                     /locus_tag="AC16HB_03185"
     CDS             588..1082
                     /locus_tag="AC16HB_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5605823.1"
                     /translation="MPNSHKVSFKLNGKGVEVAVEARELLIHTLRERLAHTGPHIGCE
                     TSHCGACTVDVDGMSVKACTVLTVQCDGAEMLTVEGLEQGGVLHALQQAFHEEHGLQC
                     GYCTPGMLTRAYRLLQENPNPSEQDIRFGLAGNLCRCTGYQNIIKAVQSAARKLASAP
                     AATA"
     gene            1136..>2820
                     /locus_tag="AC16HB_03190"
     CDS             1136..>2820
                     /locus_tag="AC16HB_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5605824.1"
                     /translation="MGDLSELQEREYKLQGMGTSLLRKEDARFIRGQGTYIDDIKLPG
                     MLFGAIVRSPFAHARIKSIDKSRALAVPGVAAVLTADDLKPVNLHWMPTLGGDVQAVL
                     ADKKVCFQYQEVAMVLASDRYAAYDGAALVEVDYEELPAIVDPKQSMAPDAPIIREDI
                     ADKKEVGHGPRTHPNHIFTWEAGNKAAADAAFAGAEVTVREEILNPRVHPCPLETCGC
                     VASFNKATGYLTVYMTTQAPHLVRTVLAMLSGIAESKIRVVGGDIGGGFGNKVPVYPG
                     YVVAIVASIVTGVPVKWIESRIDNISTTGFARDYHCVGELAAEKDGRIKGLRFTALAD
                     HGAFNSHVSASKLPAGLFSICTGSYAIPNAYARVDAVYTNKAPGGVAYRCSLRVTEAI
                     YLIERMMDVLAQKIGVDKAEIRRRNFVKTEQFPYTTAFGWTLDSGNYHAALDKVLQAV
                     DYEGLRREQAAKRADPNCPTLMGIGISTFTEIVGAGPTKVCDILGIGLFDSCEIRVHP
                     TGAVIARMGTMSQGQGHATTYAQIIATELGLPASDIVVEEGDTDKAPYGAGTWG"
BASE COUNT          528 a          942 c          933 g          417 t
ORIGIN      
        1 gcacgatcgc cgacccgcag gtgcgctaca agggcacgat cggcggcgac atttcgcatg
       61 gcgacccggg caacgatcac ccggcgctga tgattgcgct cgacgcctcg ttcgtgtcga
      121 gcgggccgaa gggcgagcgc gtggtgaagg ccgacggcta ctttctcggc ctctactcca
      181 cgctgctgga gcccaacgaa atcctgacgc agatccgcat accgatcccg gctgcgggca
      241 ccggttggtc gtaccagaag ctcaagcgca aggccggcga ctttgccacc gcggcaaccg
      301 cggtcctgct gcagatgaag ggcggcagca tcgccaaggt cgccatcgcg ctcaccaacg
      361 tcggcccgac cgcgctcaag gccagtgccg cggaggccta cctttcgggc aagacgatca
      421 acgaggccag cctgaacgag gcggcgcggc tggcgatgag catctgcgat cccgcgcccg
      481 accagcgcgg cgacgccgcc tacaagaccg cgatgtgcgg tgagatgacg cggcgtgcgc
      541 tgcaggccgc ggccgctcgt gcgaagggct gagggaggaa tcgagccatg ccgaactcac
      601 acaaggtgtc gttcaagctc aacggcaagg gagtcgaggt cgcggtcgaa gcacgcgaac
      661 tcctgatcca cacgctgcgc gagcggcttg cccacaccgg tccgcacatc gggtgcgaaa
      721 cgtcgcactg cggcgcctgc acggtcgacg tggacggaat gtcggtcaag gcctgcacgg
      781 tgctgaccgt gcagtgtgac ggtgccgaga tgctgaccgt cgaggggctc gagcaaggcg
      841 gcgtgctgca tgcgttgcag caggcgtttc acgaggagca cggcctgcaa tgcggctact
      901 gcacccccgg gatgctgacc cgcgcgtacc gcttgctgca ggagaacccc aaccccagcg
      961 agcaggacat ccgcttcggc ctcgccggca atctgtgccg ctgcaccggc taccagaaca
     1021 tcatcaaggc cgtgcagtcg gccgcccgca agctcgcgtc ggcgccagcc gcgacggcct
     1081 gaaaagcttt ttcgcgcgac gcgtacgcac cctcatcgga tcacggagac atttcatggg
     1141 cgatctgtcc gaactccagg aacgcgaata caaactgcag ggcatgggca cctcgctgct
     1201 gcgcaaggag gatgcacgct tcatccgcgg ccagggcacc tatatcgacg acatcaagct
     1261 gccggggatg ctgttcggcg cgatcgtgcg cagcccgttc gcgcatgcgc gcatcaagtc
     1321 gatcgacaag tccagggcac tcgcggtgcc cggcgtcgca gcggtgctga ccgccgacga
     1381 tctgaagccc gtgaacctgc actggatgcc gacgctcggc ggcgacgtgc aggccgtgct
     1441 ggccgacaag aaggtgtgct tccagtacca ggaagtcgcc atggtgctcg cgtccgaccg
     1501 ctacgccgct tacgacgggg cggcgctggt cgaagtcgac tacgaggaac tgccggccat
     1561 cgtcgatccg aagcagtcga tggcacccga cgcgccaatc attcgcgagg acatcgccga
     1621 caagaaggaa gtcggtcacg gaccgcgcac ccatcccaac cacatcttca cgtgggaagc
     1681 cggcaacaag gcagccgcgg acgcggcatt tgccggcgca gaggtgacgg tacgggagga
     1741 gatcctcaat ccgcgcgtgc acccctgccc actggagacc tgcggctgcg tcgcatcgtt
     1801 caacaaggcg accggctacc tcacggtcta catgacgacg caggcgccgc acctcgttcg
     1861 caccgtgctg gcgatgctgt cgggcatcgc tgaaagcaag atacgcgtgg tcggcggcga
     1921 tatcggcggc gggttcggca acaaggtgcc ggtgtatccg ggctacgtgg tcgcgatcgt
     1981 cgcttcgatc gtcaccggcg tgccggtgaa gtggatcgag tcccgcatcg acaacatttc
     2041 gaccaccgga ttcgcgcgcg actatcactg cgtcggcgag ctggcggccg aaaaggacgg
     2101 gcgcatcaag ggcctgcgtt tcaccgcgct ggccgatcac ggggcgttca attcgcacgt
     2161 gtcggcgagc aagctgccgg ccggactgtt ctcgatctgc accggctcgt atgcgatccc
     2221 caacgcctat gcgcgcgtcg acgcggtcta tacgaacaag gctccgggcg gcgtggccta
     2281 ccgctgctcg ctgcgggtga ccgaggcgat ctacctgatc gagcgcatga tggacgtgct
     2341 ggcgcagaag atcggcgtcg acaaagccga gatccggcgc cgcaacttcg tcaagaccga
     2401 gcagttcccc tacaccaccg cgttcggctg gacgctcgac agcggcaatt accacgccgc
     2461 gctcgacaag gtgctgcagg cggtcgacta cgaaggcctg cgccgcgagc aggccgccaa
     2521 gcgcgcggat ccgaactgcc cgacgctgat gggcatcggc atctcgacct tcaccgagat
     2581 cgtcggcgcc gggccgacca aggtgtgcga catcctcggc atcggactgt tcgacagctg
     2641 cgagatccgc gtgcatccga ccggagccgt cattgcgcgc atgggcacga tgagccaggg
     2701 tcagggccac gcgacgacct acgcgcagat catcgccacc gagctgggcc tgccggccag
     2761 cgacatcgtg gtcgaggaag gcgacaccga caaggcgccc tacggcgccg gcacctgggg
//
LOCUS       DBNHBY010000135         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_7107, whole genome shotgun sequence.
ACCESSION   DBNHBY010000135 DBNHBY010000000
VERSION     DBNHBY010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_7107"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..913
                     /gene="glxR"
                     /locus_tag="AC16HB_03195"
     CDS             20..913
                     /gene="glxR"
                     /locus_tag="AC16HB_03195"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HIR5605825.1"
                     /translation="MSKLGFVGLGIMGAPMASHLIKAGHEVFLFSRSGVPQSLRELGG
                     RACANGKEVGANADVIFTMVPDTPHVEEALFGANGIAAGLSKGKTVVDMSSISPLATK
                     QFAQRINELGCDYLDAPVSGGEVGAKNATLSIMVGGAQATFDRIKPLFELMGKNITLV
                     GGNGDGQTAKVANQIIVALNIEAVGEALLFAARAGADPAKVRQALMGGFASSKILEVH
                     GERMIKRTFDPGFRIELHQKDLNLALGTARQLGLSLPNTATAQELFNSCAAHGGKAWD
                     HSGLVRALEKLANFEIGQKVG"
     gene            924..>1807
                     /locus_tag="AC16HB_03200"
     CDS             924..>1807
                     /locus_tag="AC16HB_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR5605826.1"
                     /translation="MSAAPRELLRRMFDAAVAASQPELVVPKHLPATPNGRLVVLGAG
                     KASAAMARAVDDHWQGPLEGLVVTRYGYAVPCRRIEIVEAAHPVPDTAGERAARRMLA
                     IAASLGADETVLFVISGGGSALLPLPLPGLTLADKQELNRALLASGATITEMNCVRRH
                     LSAIKGGRLAAACAPAKVVSLLMSDVPGDDPIDIASGPTVPDPTTCADALAIAQRYGV
                     PLPAHVREVLESGRGESVKPGDPRLAASETQIVAAPQAALEAAARVARQAGVPAHLLC
                     DALEGEARDLGKALAGIAL"
BASE COUNT          297 a          599 c          650 g          261 t
ORIGIN      
        1 gcattgaacg gagaacggca tgagcaaact gggatttgtc ggcctcggca tcatgggtgc
       61 gccgatggcg agccacctga tcaaggccgg gcacgaagtt ttccttttca gtcgctcggg
      121 ggttccgcag agcctgcgcg aactcggcgg ccgggcatgc gcgaacggca aggaagtcgg
      181 ggcgaatgcg gacgtgatct tcacgatggt gcccgacacg ccgcacgtcg aagaggcgct
      241 gttcggcgcc aacggcatcg cggcaggcct gtcgaagggc aagaccgtgg tcgacatgag
      301 ctcgatctcg ccgctcgcaa ccaagcagtt cgcacaaagg atcaacgagc tgggctgcga
      361 ctatctcgac gcgcccgtct ccggcggcga ggtcggcgcg aaaaatgcca cgctgtcgat
      421 catggtcggc ggcgcgcagg cgaccttcga tcggatcaag ccgctgttcg agctgatggg
      481 caagaacatc acgctggtcg gcggcaacgg cgacggtcag accgccaagg tcgccaacca
      541 gatcatcgtt gcgttgaaca tcgaagcggt gggcgaggcg ctgctgttcg ccgcccgggc
      601 cggcgccgat ccggccaagg tgcggcaggc gctgatgggc ggattcgcgt cgtcgaagat
      661 cctcgaagtg cacggcgagc gcatgatcaa gcgcacgttc gacccgggct tccgcatcga
      721 gctgcaccag aaggatctga accttgcctt gggcacggcg cgccagctcg gactgagcct
      781 gccaaacacc gcgaccgcgc aggagctgtt caactcgtgc gccgcgcacg gcggcaaggc
      841 ctgggatcac tcgggcctgg tgcgggcgct ggagaagctg gcgaacttcg agatcggcca
      901 gaaggtcggc tgacggcacg acgatgagcg ccgcgccgcg cgagttgctg cgccggatgt
      961 ttgacgccgc cgtggccgcg tcgcagcccg agctcgtcgt gccgaagcac ctgccggcca
     1021 cgccgaatgg caggctggtc gtgctcggtg caggcaaggc ttcggccgcg atggcgcgcg
     1081 cggtggacga tcattggcag ggcccgctgg aaggcctcgt cgtgacgcgt tacggctacg
     1141 cggtgccatg ccggcgcatc gagatcgtcg aggcagcgca tccggtcccg gatacggccg
     1201 gcgaaagggc cgcgcgacga atgctcgcga tcgccgcatc gctcggcgcc gacgaaaccg
     1261 tgctgttcgt gatctcgggc ggcggctccg cgctgttgcc gctgcccctg ccgggattga
     1321 cgctcgccga caagcaggaa ctgaatcgcg cgctgctcgc ctcgggcgcg acgatcaccg
     1381 agatgaactg cgtgcgtcgg cacctgtcgg cgatcaaggg cggccgcctt gccgcggcat
     1441 gcgctccggc aaaggtcgtc tcgctgctga tgtcggacgt gcccggcgac gatcccatcg
     1501 acatcgcttc cggaccgacg gtgcccgacc cgacgacctg tgccgacgcg ctggcgatcg
     1561 cgcagcgcta cggcgtgccg ctgcccgcgc acgtgcgcga ggtgctggaa agcggccgcg
     1621 gcgagagcgt gaagcccggc gatccgcggc tggcggccag cgaaacgcag atcgtcgcgg
     1681 cgccgcaggc cgcgctcgaa gcagccgcgc gcgtggcgcg gcaggccggg gtacccgccc
     1741 acctcctctg cgacgctctc gagggcgagg cacgcgacct cggcaaggcg ctcgccggca
     1801 tcgcgct
//
LOCUS       DBNHBY010000136         9972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1766837, whole genome shotgun sequence.
ACCESSION   DBNHBY010000136 DBNHBY010000000
VERSION     DBNHBY010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9972
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1766837"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC16HB_03205"
     CDS             <1..177
                     /locus_tag="AC16HB_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605827.1"
                     /translation="YHVPKIAGQSEKYIEAALHAYKKGDRDHPTMRAIAASLSDEQIA
                     ALAAYYAARGAEAR"
     gene            187..513
                     /locus_tag="AC16HB_03210"
     CDS             187..513
                     /locus_tag="AC16HB_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605828.1"
                     /translation="MKRLMIAAALGIALAGSAWAADTEAGRKIADAQCAACHGKDGKT
                     PVDPTYPKLAGQYPDYLAKALNDYASGARKNAIMGGMAKPLSKSDVQNLAAYFASLPG
                     PLTHRK"
     gene            complement(560..1003)
                     /locus_tag="AC16HB_03215"
     CDS             complement(560..1003)
                     /locus_tag="AC16HB_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HIR5605829.1"
                     /translation="MFNPSRAEVRGFFCDAWRKQNAGELLTPLEGLALKWIAAHPEYQ
                     GLLADPEHALAQDFGPERGVTNPFLHLSMHLALEEQLSIDQPPGIRAAFAQLAGRAGS
                     EHDAAHEAMECLGEIVWRAQRGTLPADTSAINAAYLDCLQRRVGT"
     gene            complement(1009..1647)
                     /gene="nth"
                     /locus_tag="AC16HB_03220"
     CDS             complement(1009..1647)
                     /gene="nth"
                     /locus_tag="AC16HB_03220"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR5605830.1"
                     /translation="MNADKRAQIFATLRAANPHPTSELEFRSPFELLVAVVLSAQATE
                     RSVNLATRELFKVASTPAAIAALGEDGLIPYIRTIGLFRTKAKNIARLAQLIVERHGG
                     EVPRAREALEQLPGVGRKTANVVLNVAFGEPTIAVDTHIFRVSNRTGIAPGRNVAEVE
                     RRLEKFVPDEFRQHVHHWLILHGRYVCKARRPECWRCGIAQWCEHRPKTAAP"
     gene            complement(1647..2264)
                     /gene="rsxB"
                     /locus_tag="AC16HB_03225"
                     /pseudo
     CDS             complement(1647..2264)
                     /gene="rsxB"
                     /locus_tag="AC16HB_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
     gene            complement(2272..3192)
                     /locus_tag="AC16HB_03230"
     CDS             complement(2272..3192)
                     /locus_tag="AC16HB_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HIR5605831.1"
                     /translation="MLCALLIAVLLAGCATPPQTARLTAHAPAGLPRQALLSHVPFFA
                     QEQFQCGPAALAMTLNASGLATTPDMLQPQVYLPAREGSLQPEMLAAARRNGRLPVVL
                     APRLDAVLTEIADGRPVIVLQNLSLPILPQWHYAVAIGYDLDRGELVLHSGVTEGQRL
                     TLQVFERTWARSGYWAMVADAPAKLPRTPAPEALLAAAIALERVDATAARQAYQALTE
                     RAPRMTLAWIGLGNTAFASGDREQAASAFEHATVLDPQAADAWNNLANALAALGRREE
                     ARSAARRAIALGGPRLERYRETLSAIDRLR"
     gene            complement(3237..3623)
                     /locus_tag="AC16HB_03235"
     CDS             complement(3237..3623)
                     /locus_tag="AC16HB_03235"
                     /inference="COORDINATES: protein motif:HMM:NF042483.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR5605832.1"
                     /translation="MTFFRRTIATVAAVCVLGVSLPLPAQAGMVGTDEIAQPSTTSAQ
                     ARLSQLLAREDVRDALVARGVDPREVQARVDALTADEAQLLAQRLDQLPAGGDVIGVI
                     LFIFVLLLITDILGLTKVFPFTRPIR"
     gene            complement(3701..4936)
                     /locus_tag="AC16HB_03240"
     CDS             complement(3701..4936)
                     /locus_tag="AC16HB_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR5605833.1"
                     /translation="MLYDLHDLQRKFMNPLAMWAQATSEMFSSPYSPLSYTPFSRRVA
                     AGYDLLHRIGKQYEKPGFELSPTVVDGRKVPVKEEVVVTKPFCKLLHFRRGFGNGQAK
                     RNEDPTVLVVAPLSGHHATLLRDTVRALLPEHDVYITDWIDARLVPIAHGPFHLDDYV
                     DYVIEFVRLLGPDVHVISVCQPTVPVLAAISLMATNKDPASPRTMTMMGGPIDTRRNP
                     TQVNALATRKPIEWFENNVIFKVPSTHPGYLRSVYPGFLQHAGFVAMNPDRHLNSHWD
                     YYLDLVKGDLEDVESHRYFYDEYNAVIDLPAEYYLDTIRIVFQEQALPRGKWYVRGLR
                     VAPEDIRNTALFTIEGELDDISGSGQTQAAHDLCRSIPKSRHRHLTARGVGHYGIFSG
                     RRWREEIYPEIRDFIRANV"
     gene            complement(5062..5862)
                     /locus_tag="AC16HB_03245"
     CDS             complement(5062..5862)
                     /locus_tag="AC16HB_03245"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR5605834.1"
                     /translation="MVAPDKYTAERIVWIHPWAPNLFSFRLTRYAGFRFAPGQFARLG
                     VAKPDPDNPKNPSGTRIVWRAYSIVSASDDAQLEFYSIVIPGGDFTSELARLRVGDIV
                     YVEKLNYGFLTCDRFEGGKDLWLLATGTGLAPFISILWDPKTWDDYENLILVHSARYP
                     NELAYQATIAAFSTHERFRAHAGKLRYVPVVTRAKVDGALDQRIPALLDSGRLESQVG
                     LGLDPARSRVMICGNPDMITDTRKRMSTRGFATSRRGIPGQLAVENYW"
     gene            complement(5879..6202)
                     /gene="fdxA"
                     /locus_tag="AC16HB_03250"
     CDS             complement(5879..6202)
                     /gene="fdxA"
                     /locus_tag="AC16HB_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR5605835.1"
                     /translation="MTHVVTEPCIKCKYTDCVDVCPVDCFREGPNFLAIEPDECIDCA
                     VCIPECPVNAIYAEEDVPEDQRHFTKLNAELSRKWPSITRMKAHPADADEWKDVRQKE
                     HLLER"
     gene            6296..7525
                     /locus_tag="AC16HB_03255"
     CDS             6296..7525
                     /locus_tag="AC16HB_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5605836.1"
                     /translation="MQLSFKRRTLIAAGASALVAPSVRAQPTRGSAPIRIGEINSYST
                     IPQFLVPYRKAWELAVEEVNAAGGLLGRKVEVISRDDAGKPDDAIRHATELVANERVD
                     LLAGTFLSNVGLAVADFALRNRRLFVASEPLTDALVWDKGNRYTFRLRPSTYMQSAML
                     VEEAVKLPAKRWVTIAPNYEYGQSAVASFKALLKAKRPEVEFVAEQWPAQGKLEAGST
                     VQAVLSARPEAVFNVAFGADLTRLVREGNLRGLFPRVPTVSLLSGEPEYLDALKDETP
                     KGWIVTGYPWDQIATPEHAKFFNAYYRRNQDTPKLGSVVGYSAMQAIFEAIRKARSTD
                     NEKLIAALRGLSFATPFGAATFRALDQQSTMGAYVGKLDVRGDKGTMVDWRYADGAKY
                     LPSDDEVRRRRPAEAMK"
     gene            7633..8523
                     /locus_tag="AC16HB_03260"
     CDS             7633..8523
                     /locus_tag="AC16HB_03260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5605837.1"
                     /translation="MQVPTVSLGLPVFNGEAFLARALDCVLAQTWTDFELVVSDNAST
                     DATATIIADYTARDARIRGFRQPRNIGSGNNWTFVARQARGRYFKWVAADDEFEPRLL
                     VDCIEVMERDPAVVLCYGRTRFIDLAGGRLDVYRGDFEVQADDPIERYRVVRERLHLG
                     TPIQAGVIRTAALRRCGYLGNYPDSDRVLIAGLALEGKFVLLPRVLFYRRWDRSVASA
                     LRSPLELAQLYRPGTTAAPRFASLPRQLGQLRVALKTPPNLGAKLAALYAVVRYTDWR
                     RKLGVVGRRASGVGRTNCGG"
     gene            8623..>9972
                     /locus_tag="AC16HB_03265"
     CDS             8623..>9972
                     /locus_tag="AC16HB_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605838.1"
                     /translation="MTQFLAQLLTGLAYASTLFLVAAGLTLIFGVTRVVNFAHGSLYM
                     LGAYLASTLTAALPLTVVGFFGGIVLAALIVAAIGIVIEVLVLRRIYAAPEIFQLLAT
                     FGVVLIVQDLALALWGPEDLFAPRAPGLKGSVEIAGALVPQYTLFLLVIGPLMFAALW
                     LVFRRTRWGIQVRAATQDGEMAGALGVNRALLFTTVFALGSFLAGLAGALQIPRESVN
                     LQMDLGVIVEAFVVVVVGGLGSIGGAALASILIGLLHAFGVWFLPQSALVLAFVAMAI
                     VLVVRPFGLLGRAEVVAHTPRGAHAPFRPAPAALKWLAVIATAAAASAPLWAGEYLLV
                     ILTEMAIVAVFAASLHFFMGLGGITSFGHAAFFGLGAYGAALLAKHAAAPFALGLVAG
                     PLAAFIGGIAAGWFVLRAQGVYAAMLTLAFAQILWSVAVQWIEVTGGDNGVLGLWPPD
                     "
BASE COUNT         1517 a         3286 c         3480 g         1689 t
ORIGIN      
        1 tatcacgtgc cgaagatcgc cggccagtcg gagaagtaca tcgaggccgc gctgcacgcc
       61 tacaagaagg gtgaccgcga tcatccgacg atgcgcgcca tcgccgcgag cctgagtgac
      121 gaacagatcg ccgcgctcgc cgcctattac gcggcgcgcg gtgcggaagc caggtaagga
      181 gtgacgatga agcgactcat gatcgccgct gcgctcggca tcgcgctcgc gggcagcgcc
      241 tgggcggccg acaccgaggc cggccgcaag atcgccgacg cgcagtgcgc cgcgtgccac
      301 ggcaaggacg gcaagacgcc cgtcgatccg acgtatccga aactggccgg gcagtacccc
      361 gactacctcg ccaaggcact gaacgactac gccagcggcg cacgcaagaa cgcgatcatg
      421 ggcggcatgg ccaagccgct gtccaagagc gacgtgcaga acctggccgc gtatttcgcc
      481 agcctgccgg ggccgctgac gcatcgcaag tagccggtac ggtgttgcat gcgacgggcg
      541 ctgcggcgcc cgtcgtcttt cacgtgccca cccggcgctg caggcagtcg agataggcgg
      601 cgttgatcgc cgaggtgtcg gccgggagcg ttccccgctg cgcgcgccat acgatttcgc
      661 ccaggcactc catcgcttca tgcgcggcgt cgtgctccga tccggcgcgc ccggccaact
      721 gtgcgaaagc ggcgcggatt cccggcggct ggtcgatcga caactgctcc tccagcgcga
      781 gatgcatcga cagatgcagg aatggattgg tcacgccacg ctcgggaccg aagtcctgcg
      841 ccaacgcgtg ctccggatca gcgagcagtc cctggtattc cggatgcgcg gcgatccatt
      901 tcagcgccag cccttcgagc ggcgtcagca gttcgcctgc gttctgcttg cgccacgcgt
      961 cgcagaagaa accgcggacc tctgcgcgcg acgggttgaa cagcgtcgtc atggcgccgc
     1021 ggtcttgggc cgatgctcgc accattgcgc gatcccacag cgccagcatt ctggcctgcg
     1081 tgccttgcac acataacggc cgtgcaggat cagccagtgg tgcacgtgct ggcggaattc
     1141 gtccggcacg aacttctcca gcctgcgctc gacctcggcg acgttcctgc cgggtgcgat
     1201 cccggtgcgg ttcgatacgc ggaagatgtg ggtgtcgacc gcgatcgtcg gctcgccgaa
     1261 cgcaacgttc aaaacgacgt ttgctgtctt gcggccgacg cccggcaact gttccagcgc
     1321 ctcgcgcgcg cgcggcacct cgccgccgtg ccgctcgacg atcagctgcg ccaaccgtgc
     1381 gatgtttttc gccttggtgc ggaacaggcc gatggtccgg atgtatggga tcagtccatc
     1441 ctcgccgagc gcggcgatcg ccgccggcgt gctggcaacc ttgaacagtt cgcgcgtcgc
     1501 caggttcacg cttcgttcgg ttgcttgcgc ggacagcacg accgcgacca gcagttcgaa
     1561 cggcgagcgg aattcgagct cggacgtggg gtgcggattg gccgcacgca gcgtggcaaa
     1621 gatctgcgcg cgcttgtcgg cgttcacgcc gcgtacccct cgcggcgacg gcgcgcgcgt
     1681 gcgagcgcgg cggcgacgag cgcctgcttg tcgttcgccg gcggcggtat gggtgctcga
     1741 actgcgcgcc gcgcgtcgtt cgccgccggc gcgcgcgcca gccgcgcgtt gcgcgcgtgg
     1801 tggcgcgcac gggccgcgtt agcgtcgtcg cgcgtccacg cacggccgtg ttcgaccggc
     1861 accatcgcga tgcagtccat cgggcagggc ggcaggcaca ggtcgcagcc cgtgcacagc
     1921 cgcggaacga cggtgtgcat cagcttggca gccccgacga tcgcgtcgac cgggcaggcc
     1981 tggatgcaca acgtgcagcc gatgcagcgc gcttcgtcga tcagcgcgat tcgcagcggc
     2041 tgctcggcgc cgttggcgga gttcatcggc agcgcgggcc ggccaagcaa cgcggccagc
     2101 tttgcgatgc ccgcggctcc gccgggcggg cactggttga tctcggccgc gccgctcgcg
     2161 atcgcgtccg cgtacggacg gcagcctgcg tggccgcact tcgtgcattg cgtctgcggc
     2221 agcagcgcat cgatcgcatc cgccagcgcc ttggtgtcgg gcatgtggca attatcgcaa
     2281 gcggtcgatc gccgacagcg tctcgcgata gcgctcgagg cgtggcccgc ccaacgcgat
     2341 tgcgcgccgc gcggcgctgc gcgcctcctc gcggcgaccg agcgcggcaa gtgcattggc
     2401 gagattgttc catgcgtccg ccgcctgcgg gtcgagcacg gtcgcgtgct cgaaggcgga
     2461 tgcggcctgt tcgcggtcgc cgcttgcgaa tgcagtgttg ccgagaccga tccacgccag
     2521 cgtcatccgc ggcgcgcgct cggtcagcgc ttgataggct tgccgtgccg ccgtcgcgtc
     2581 gacgcgctcc agcgcgatcg cggctgcgag cagcgcttcc ggcgccggcg tgcgcggcag
     2641 ctttgccggt gcgtcggcga ccatcgccca gtaaccgctg cgagcccacg tgcgctcgaa
     2701 gacttggagc gtcagtcgct gcccctcggt gacgcccgag tgcagcacca actcgccgcg
     2761 gtcgaggtca taaccgatcg cgaccgcgta atgccactgc ggcagaatcg gcagcgacag
     2821 gttctgcagc acgatcaccg gacggccgtc ggcgatctcg gtcagcactg cgtcgagccg
     2881 aggcgccagc acgaccggca gccgcccgtt gcggcgagcg gccgcgagca tctcgggctg
     2941 cagcgagccc tcgcgcgccg gcaagtacac ctgcggttgc agcatgtcgg gcgtcgtcgc
     3001 gagaccggac gcgttgagcg tcatcgcgag cgccgccggg ccgcactgga actgttcctg
     3061 cgcaaagaag ggcacgtgcg acagcagcgc ctgccgcggc aggccggcag gtgcatgcgc
     3121 cgtgagccgt gcggtctgcg gcggcgtcgc gcaaccggcg agaagcactg cgatcagcag
     3181 cgcacaaagc aagacccccg ccgcagcggg ggtcgctttc cggtcagatg ccgtgttcaa
     3241 cggatcggac gcgtgaacgg gaagaccttg gtcaggccga gaatgtcggt gatcagcagc
     3301 agcacgaaga tgaacaggat cacgccgatc acatcgccgc cggccggcaa ctggtcgagc
     3361 cgctgcgcca gcagctgggc ttcgtcggcc gtcagtgcat cgacgcgcgc ctggacctcg
     3421 cgcggatcga cgccgcgcgc cacgagcgcg tcgcgcacgt cctcgcgcgc cagcagttgc
     3481 gacagccggg cctgggccga tgtcgtcgac ggctgcgcga tttcatcggt cccaaccatg
     3541 cccgcctgtg caggcagcgg caggctcacg ccgagcacgc agacggcggc aacggtggcg
     3601 atggtgcgcc ggaagaaggt catgttgtct cctcgcagaa aggccacaag gccggaggag
     3661 ccgaagtttg ccacgcggcg cggcgcgccg catgcgcggg tcagacgttc gcccgaatga
     3721 aatccctgat ctcggggtag atctcctcgc gccagcgccg gccggagaag atgccgtaat
     3781 gaccgacgcc cctggcggtg aggtggcggt gtcggctctt cggaatcgat ctgcacaggt
     3841 cgtgcgcggc ctgggtctgg ccgctgcccg agatgtcgtc gagttcgccc tcgatcgtga
     3901 acagcgccgt attgcggatg tcctccggcg ccacgcgcag gccgcgcacg taccatttgc
     3961 cgcgcggcag cgcttgctcc tggaacacga tgcggatcgt gtcgaggtag tactcggcgg
     4021 gcaggtcgat caccgcgttg tactcgtcgt agaagtagcg atgcgactcg acgtcctcga
     4081 ggtcgccctt gaccagatcg aggtagtagt cccagtgcga gttcaggtgc cggtccgggt
     4141 tcatcgcgac gaatcccgcg tgttgcagga atcccggata cacgctgcgc aggtagcccg
     4201 gatgcgtgct cggcacctta aagatcacgt tgttctcgaa ccactcgatc ggcttgcggg
     4261 tggcgagcgc gttgacctgg gtcggattgc gtcgcgtatc gatcggcccg cccatcatcg
     4321 tcatcgtgcg cgggctggcc ggatccttgt tggttgccat cagggagatc gctgccagca
     4381 ccggcaccgt cggctggcat acggagatca cgtgcacgtc ggggccgagc aggcgcacga
     4441 actcgatcac gtaatcgaca tagtcgtcga ggtggaacgg cccatgcgcg atcggcacca
     4501 gccgcgcgtc gatccagtcc gtgatgtaga catcgtgctc ggggagcagc gcacgcacgg
     4561 tgtcgcgcag cagcgtcgca tggtgccccg acaggggcgc gaccaccagt acggtcgggt
     4621 cctcattgcg tttcgcctga ccattgccga agccgcgcct gaagtgcagc agcttgcaga
     4681 acggcttggt gacgacgacc tcttccttga ccggcacctt gcggccgtcg acgaccgtcg
     4741 ggctgagttc gaagcctggc ttttcgtact gcttgccgat ccgatgcagc aggtcgtagc
     4801 cggcggcgac gcggcgggaa aacggggtgt aggagagcgg actgtatggc gacgagaaca
     4861 tctccgaggt ggcctgggcc cacatcgcga gcggattcat gaacttgcgc tgcaggtcat
     4921 gcaggtcata cagcatccgc ggctcccccc aaggcgtcgt ctctcccacc ctgtgatcac
     4981 agatttgcgg gggattatgc gcgcggggat gcgacgcagc aaatccgccg accgccggcg
     5041 ttgcgccgac gcaatcttcg cctaccagta gttttccacc gccagctgac cggggatgcc
     5101 gcggcgcgac gtggcgaagc cacgggtcga catccgcttg cgcgtatcgg tgatcatgtc
     5161 ggggttgccg cagatcatca cacgcgaacg cgccggatcc aggcccaggc caacctggct
     5221 ttcaagccgt ccgctgtcca acaaggccgg gatgcgctga tcgagggcgc catcgacctt
     5281 tgcccgcgtc accacgggta cgtagcgcag cttgcccgcg tgggcgcgga agcgctcgtg
     5341 cgtggagaag gccgcgatcg tcgcctggta ggcaagttcg ttcggatagc gggcgctgtg
     5401 cacgaggatc aggttctcgt agtcgtccca ggtcttcggg tcccacagga tcgagatgaa
     5461 cggcgccagc cccgtgccgg tcgcgagcag ccacaggtcc ttacccccct cgaagcggtc
     5521 gcaggtcagg aagccgtagt tcagcttttc gacgtacacg atgtcgccga cccgcagccg
     5581 cgccagttcg gaggtgaagt cgccgccggg gatgacgatc gagtagaact cgagttgcgc
     5641 gtcgtcgctg gccgacacga tcgaatacgc gcgccagacg atgcgcgtgc cggacgggtt
     5701 cttcgggttg tccggatcag gctttgcgac cccaaggcgc gcaaactggc cgggcgcgaa
     5761 tcgaaatccc gcgtagcgcg tgaggcggaa cgagaacagg ttcggcgccc acggatggat
     5821 ccagacgatc ctctccgcgg tgtatttgtc tggtgcgacc acgaatgcag cgcggcgttc
     5881 agcgctccag caggtgttcc ttctgccgca cgtccttcca ctcgtcggcg tcggccggat
     5941 gcgccttcat gcgcgtgatc gacggccact tgcgcgacag ctcggcattg agcttggtga
     6001 agtggcgctg atcctccggc acgtcctcct cggcgtagat cgcgttgacg gggcactcgg
     6061 ggatgcagac ggcgcagtcg atgcactcgt cgggttcgat cgcgaggaag ttcggccctt
     6121 cgcgaaagca atcgacgggg cagacgtcga cgcaatcggt gtacttgcac ttgatgcagg
     6181 gctcggtgac gacgtgggtc atcgcgtgct cggaatcgat cgttgaaggc gcgatattct
     6241 atcgcgctgc gtcatcgaac cccgctttcc caccgacccg cccgaaggga cttcaatgca
     6301 gttgtcattc aaacgcagga cgctgatcgc cgcgggcgcc tcggcgctgg tcgcgccttc
     6361 cgtgcgtgcg cagcccaccc ggggcagcgc gccgatccgc atcggcgaga tcaacagcta
     6421 ctcgaccatt ccgcagttcc tcgtgccgta tcgcaaggca tgggagcttg cggtcgagga
     6481 ggtcaacgct gccggcggac tgctcggccg caaggtcgag gtgatctccc gcgacgacgc
     6541 gggcaagccc gacgatgcta tccggcatgc gaccgaactc gtcgccaacg agagggtgga
     6601 tctgctcgcc ggcacgttcc tgtcgaacgt ggggcttgcg gtggcggact tcgcgctgcg
     6661 caaccggcgc ctgttcgtcg caagcgagcc gctgaccgat gcgctggtat gggacaaggg
     6721 caaccgttac acgttccggt tgcggccgag cacctacatg cagagcgcga tgctggtcga
     6781 ggaagcggtc aaacttcccg cgaagcgctg ggtcacgatc gcgcccaact acgagtacgg
     6841 gcagagcgcg gtggcgagct tcaaggcgct gctgaaggca aagcggccgg aagtcgagtt
     6901 cgtcgccgag caatggccgg ctcagggcaa gctcgaggcg ggcagcacgg tgcaggccgt
     6961 cctttccgcg cggcccgagg cggtcttcaa cgtcgccttc ggggccgatc tgacgcggct
     7021 cgtgcgcgag ggcaatctgc gcggactgtt cccgcgcgtg ccgaccgtga gcctgctgtc
     7081 gggcgagccc gagtatctgg atgcgctgaa ggacgaaacc ccgaagggct ggatcgtcac
     7141 cggctacccg tgggaccaga tcgccacgcc cgagcacgcg aagttcttca acgcctacta
     7201 caggcgcaac caggacacgc cgaagctcgg ctccgtcgtc ggctacagcg cgatgcaggc
     7261 gatcttcgag gcgattcgga aagcgcgctc gaccgacaac gagaagctga tcgccgcgct
     7321 gcgcgggctg tcgttcgcca cgccgttcgg cgcggcgacg ttccgcgcgc tcgaccagca
     7381 gtcgaccatg ggcgcgtacg tcggcaagct cgatgtgcgc ggagacaagg gcacgatggt
     7441 cgactggcgt tacgccgacg gagcgaagta cctgccgagc gacgacgagg tgcgcaggag
     7501 gaggccggcg gaggcgatga aatgaggatt gcggaagtcg gattgcggct agcgcggggt
     7561 gcatgcccct gagaacgggc acgacatttg cggcgcgctt gcgtaagtgg aggtgcggtg
     7621 ccgatgccgg gcatgcaggt tccgacggtc agcctcgggc tgccggtgtt caacggcgag
     7681 gcgttcctgg cccgcgcgct ggattgcgtt ctggcgcaaa cctggaccga cttcgagctg
     7741 gtcgtctccg acaacgcgtc gaccgacgcc accgccacca tcattgccga ctacacggcg
     7801 cgcgatgcac gtattcgcgg cttccgccag ccgcgcaaca tcggcagcgg caacaactgg
     7861 acctttgtcg cccgccaggc gcgcggccgg tacttcaagt gggtcgctgc cgacgacgaa
     7921 ttcgagccgc gcctgctggt cgactgcatc gaagtgatgg aacgggaccc ggccgtcgtg
     7981 ctttgctacg gccgcacgcg cttcatcgat ctcgccggcg gccggctcga tgtctaccgc
     8041 ggcgacttcg aggtgcaagc cgatgatccg atcgagcgct accgtgtggt gcgcgagcgg
     8101 ctgcacctgg gcacgccgat ccaggccggc gtgattcgaa ccgcggcgct gcgtcgctgc
     8161 ggctaccttg gcaactatcc cgactcggat cgggttctga tcgcggggct tgcgctcgaa
     8221 ggcaagttcg tgctgctgcc gcgggtgctg ttctatcgcc gctgggacag gtcggtcgcc
     8281 tcggcgctca ggtcgccgct cgaactagcg caactgtatc ggcccggcac gacggctgca
     8341 ccgcggttcg cgagcctgcc gcgccagctg ggtcagctgc gcgtcgcgct gaagacgccg
     8401 cccaatttgg gcgccaagct ggcggcactc tacgcagtgg tgcgctacac cgattggagg
     8461 cgcaagctgg gagtcgtggg gcgtcgggcg tcgggagtcg ggcgcacgaa ctgcggggga
     8521 tgacgcacgc cgcgcttggg agccgacccg aggcttccgg ggtttggtca gccgagtgat
     8581 tgctttagtc tcctgacgcc cgacgccccg cgcccgacgc tcttgaccca gttcctcgcc
     8641 cagctcctga ccggtctcgc gtacgcgtcc acgctgttcc tggtggcggc cgggttgacg
     8701 ctgatcttcg gcgtcacgcg cgtcgtgaac ttcgcgcatg ggtcgctgta catgctcggc
     8761 gcctacctgg cgtcgacgct gaccgcggcg ctgccgctga cggtggtcgg gttcttcggt
     8821 ggcatcgtgc tcgccgcgct gatcgtcgcc gcgatcggca tcgtgatcga ggtgctggtg
     8881 ctgcggcgga tctacgccgc gccggagatc ttccagttgc ttgcgacctt cggcgtggtg
     8941 ctgatcgtgc aggatctcgc acttgcgctc tgggggccgg aggatctgtt cgcgccgcgc
     9001 gcaccggggc tgaagggcag cgtggaaatc gcgggcgcgc tggtgccgca gtacacgctg
     9061 tttctgctcg tgatcggccc gctgatgttc gcggcgctgt ggctcgtttt ccgccgcacg
     9121 cgctggggca tccaggtgcg ggccgccacg caggacggcg agatggccgg tgcgctcggc
     9181 gtgaaccgcg cgctgctgtt cacgaccgtg ttcgcgctcg gcagttttct cgccggactg
     9241 gccggcgcgc tgcagatccc gcgcgagtcg gtgaacctgc agatggatct gggcgtgatc
     9301 gtcgaggcat tcgtcgtggt ggtggtcggc gggctgggct cgatcggcgg ggccgcactg
     9361 gcttcgatcc tgatcggact gctgcacgcg ttcggcgtct ggttcctgcc gcagtcagcc
     9421 ctggtgctgg cgttcgtcgc gatggcaatc gtgctggtcg tgcggccgtt cggcctgctc
     9481 ggccgggccg aggtcgtcgc acacacgccg cgcggcgcgc acgctccgtt ccggccggcc
     9541 cccgcggcac tcaaatggct cgcggtcatt gcgaccgccg cggcggccag tgcgccgctg
     9601 tgggccggcg aatacctgct ggtgatcctg accgagatgg cgattgtcgc ggtgtttgcc
     9661 gccagcctgc acttcttcat ggggctgggc ggcatcacgt cgttcggcca tgcggcgttc
     9721 ttcgggctgg gcgcgtacgg cgccgcactg cttgccaagc atgcagccgc gccgtttgcg
     9781 ctcggcctcg tcgccggtcc gctcgcggcg ttcatcggcg gcatcgcggc cggttggttc
     9841 gtcttgcgcg cgcagggcgt gtacgcggcg atgctgaccc tcgcgttcgc gcagatcctg
     9901 tggtcggtgg cggtgcagtg gatcgaggtg acgggcggcg acaacggtgt cctcggcctg
     9961 tggccgccgg at
//
LOCUS       DBNHBY010000137         1708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1563723, whole genome shotgun sequence.
ACCESSION   DBNHBY010000137 DBNHBY010000000
VERSION     DBNHBY010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1563723"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16HB_03270"
     CDS             complement(<1..376)
                     /locus_tag="AC16HB_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5605839.1"
                     /translation="MDLPSLLIQILNSVQYGLLLFLVASGLTLIFGIMGIINLAHGSF
                     YMVGAYMAFVLTSATGSFALAIALGVPLALALGWLLEWLVFAHLYRRDHLEQVLLTYG
                     LILFFEEARSILVGDDVHSVAIP"
     gene            complement(439..1620)
                     /locus_tag="AC16HB_03275"
     CDS             complement(439..1620)
                     /locus_tag="AC16HB_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5605840.1"
                     /translation="MKTNQTRRATVGAIAGGTALAALPFATFAQGRKLKIGLMLPYTG
                     TYAQLGEAITNGFKLYVAETGGKLGGMDVEFVTVDDESAPPKAVENMNRLVRRDNVDV
                     VVGTVHSGVALAMANVARQTNTTLIIPNAGADDITGSMCAPNIFRTSFTNWQPGYGMG
                     KVMADRKIKSAVTLTWKYAAGEESVNGFKEAFTAAGGKIVKDMTLPFPNVEFQPFLTE
                     IASLKPEAVFVFFAGAGAAKFVKDYYAAGLKSIPLYGSGFLTDGVGEAIQDVAQGLTT
                     VLHYGDGIDNPKNVAFRKAYAVKYKLQPDVYAVQGYDAAQLLAAGLRAVKGDPSKKAE
                     MYKAMEAAKIDSPRGPFTLSKAHNPVQDMYIRQLKGRDNVVTGIAVKALADPARGCKA
                     A"
BASE COUNT          279 a          563 c          551 g          315 t
ORIGIN      
        1 cagggatcgc cacgctgtgc acgtcgtcgc cgaccaggat gctgcgcgcc tcctcgaaga
       61 acaggatcag cccgtaggtg agcagcacct gctcgaggtg atcgcgcctg tacaggtgcg
      121 cgaacacgag ccattcgagc agccagccca gagcgagcgc gagcggcacg ccgagcgcga
      181 tggccagcgc aaagctgccg gtcgcgctcg tcagcacgaa cgccatgtac gcgccgacca
      241 tgtagaagct gccgtgcgcg aggttgatga tgcccatgat cccgaagatc agcgtcagcc
      301 cgctggcgac gaggaacagc agcaggccgt actgaaccga gttcaggatc tggatcagga
      361 gcgaaggcaa atccatcaac aaaaactccc ggcgccttgt gggaacgccg ggagtctatg
      421 cgcgggatgg aggcgcggct acgctgcctt gcaaccgcgc gccggatcgg cgagtgcctt
      481 gaccgcgatg ccggtcacga cgttgtcgcg tcccttcagc tgccggatgt acatgtcctg
      541 caccgggttg tgcgccttcg acagcgtgaa cgggccgcgc gggctgtcga tctttgccgc
      601 ttccatcgcc ttgtacatct cagccttctt cgacgggtcg cccttgaccg cgcgcagccc
      661 cgcggcgagc agctgtgcgg cgtcgtagcc ctgcaccgcg tagacgtccg gctgcagctt
      721 gtacttgacc gcgtacgctt tgcggaacgc gacgttcttc gggttgtcga tgccgtcgcc
      781 gtagtggagc accgtggtca gtccctgcgc cacgtcctgg atcgcctcgc ccacgccatc
      841 ggttaggaag ccggatccat atagcggaat gctcttcagc ccggccgcgt agtagtcctt
      901 gacgaacttg gccgcgccgg cgccggcaaa gaacacgaac accgcctccg gtttcagtga
      961 cgcgatctcg gtcaggaacg gctggaactc gacattcggg aacggcagcg tcatgtcctt
     1021 gacgatcttg ccgccggcgg cggtgaacgc ctccttgaat ccgttgaccg actcttcgcc
     1081 ggccgcgtac ttccaggtca gcgtgacggc gcttttgatc ttgcggtcgg ccatcacctt
     1141 gcccatgccg tagcccggct gccaattggt gaacgaggtg cggaagatgt tcggcgcgca
     1201 catcgagccg gtgatgtcgt cggcgcccgc gttcgggatg atcagcgtcg tgttggtctg
     1261 gcgcgcgacg ttggccatcg ccaacgccac gcccgagtgc acggtgccga ccaccacgtc
     1321 gacgttgtcg cggcgaacca gtcggttcat gttctccact gccttgggcg gcgccgattc
     1381 gtcgtcgacg gtcacgaact cgacgtccat gccgccgagc ttgccgccgg tttcagcgac
     1441 gtacagcttg aagccgttgg tgatcgcctc gccgagctgc gcgtacgtgc cggtgtacgg
     1501 cagcatcagg ccgatcttga gcttccggcc ctgggcaaac gtcgcgaacg gcagcgccgc
     1561 aagtgccgtg cccccggcaa tcgcgccgac ggtggcgcga cgcgtctggt tggtcttcat
     1621 ccgtgtctcc ttgtgcaaga aatcgtccgg tcgcagtgta ctttcgcggt gcgacgaggg
     1681 gagcggcatt tacccgcccg ccgcctgc
//
LOCUS       DBNHBY010000138         3546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_819211, whole genome shotgun sequence.
ACCESSION   DBNHBY010000138 DBNHBY010000000
VERSION     DBNHBY010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_819211"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC16HB_03280"
     CDS             complement(<1..532)
                     /locus_tag="AC16HB_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5605841.1"
                     /translation="MNRTLALFAASIAVAFAPAGHAGNEFAKATSTAAGYSIWDADLI
                     NVETVAQTGSGVFVAVLDTGLVPNWRDYFPRARIATNLGTGFDQSVVFRMASGNNPCG
                     LEAEVGRLRQTTWVGSTGSTHGTHVVSTIVGYNYQSPSDAVAGFVLPPIQVRGIAPDV
                     TIIPVKVLADYQIPPAP"
     gene            764..2848
                     /locus_tag="AC16HB_03285"
     CDS             764..2848
                     /locus_tag="AC16HB_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HIR5605842.1"
                     /translation="MNQWHRALLPAALLSCVTAASAQGGQFSHAPNKLPAGVTPYSAI
                     HYDGSSDDLLTAGLGKTGLAGARPAPADVNDPAQIRKLVIWTNYRAVLDITAAGGFGV
                     LYGPNIDVNGNDTLGEGKVAGTEYIAVFDRGASGRNVTLMVQVPDAFDPDHACIVTGT
                     SSGSRGVYGAIGSSGEWGLKRGCAVAYADKGTGNGVHDLQNNTVNRINGVRADAAAAG
                     DDTVFTAPISALERQQFNADTPNRFAVKHAHSQLNPEKDWGRHTLTAIEYAFYVLNEK
                     FGTERKGKGIARTITPANTIVIASSISNGGGAALQAAEQDRAGLIDGVAVTEPNIQVM
                     HPQNPPIRRGAVTYTGGSKPLYDFFTFENLYQPCAALAPAAAAGPLSVLVNATRAANR
                     CQSLAEKGLLSSTTLAAQAQEALDKLIAYGWQPESTPLHASHWALATPSITMTYSNAY
                     GRFSVLDNLCGFSFAATDAATGNVLSTTPALLATVFGDGNGVPPTIGINIVNNLSPGG
                     AKADGLSKSPSTNREDFNLDGALCQRNLWTGSDTNAQRVRAGIAEVQQSGSLRGKPSI
                     IVHGRADALVPVNFSSRAYVAQNHWREGDATNLRYIEVTNAQHFDAFLGLAGYNSAFV
                     PLHVYFVRAMDAMWAHLTQGTPLPPSQVVRTTPRGAGAPPITGANVPAIASAPGAADR
                     VTFDGDTLVIPD"
     gene            2955..>3546
                     /locus_tag="AC16HB_03290"
     CDS             2955..>3546
                     /locus_tag="AC16HB_03290"
                     /inference="COORDINATES: protein motif:HMM:NF052711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5605843.1"
                     /translation="MATGNEPSSLSKLAIDRSAAPIRRRRPLWKRWWVWLLALIAAGA
                     AAAFVSQRGRPLPFEIGAVVAAYPTQSIAMLNATGRVSAARRASVSSKATGRLEWLGV
                     QEGQRVQEGEIIARLENRDVAAQREQAAANVKAAQANLVQGQAELADAEQAYKRAQDL
                     LQKNFVSESSVDTALARYNKARAATESLKAQIAVAEA"
BASE COUNT          617 a         1201 c         1207 g          521 t
ORIGIN      
        1 gcggggcggg cgggatctgg taatccgcca gcaccttgac cgggatgatc gtcacgtcgg
       61 gcgcgatgcc gcgcacctgg atcggcggca atacgaatcc ggccaccgca tcgctcgggc
      121 tctgatagtt gtaaccgacg atggtgctta cgacatgcgt gccgtgtgtg gaccccgtcg
      181 atccaaccca agtcgtctgg cgcagccggc cgacctcggc ctcgagtcca cacggattgt
      241 tgccgctggc catgcggaag acgaccgact gatcgaagcc tgtgccgaga ttggtcgcga
      301 tgcgcgcgcg cggaaaatag tcgcgccaat tggggaccag acccgtgtcc agcacggcca
      361 caaatacgcc gctgccggtc tgcgcgacgg tttcgacgtt gatcagatcg gcgtcccaga
      421 tcgagtaccc cgctgccgtc gaagtcgctt tggcgaactc gttgccggcg tggcccgcgg
      481 gcgcgaatgc gacagcgatg ctggcggcga acagtgcgag cgttcggttc atggacatcc
      541 cctcaattga gcgacgacgc gcccgagtgc gggcatgctc gcgccgcgcg gaggacggtg
      601 tcaaagccga tcggcaagca cgcacatgaa tccgacgaaa tcgcgatttg ttagcgctcg
      661 cacacgattg tgcgagtcgc gaagttgcgt caagctgcgc agcccgagcc gcacgatcga
      721 taacaaggcg gccccattga agtcggaagt cgaggagaac acgatgaacc aatggcaccg
      781 cgcgctgctg ccggcagcgc ttctttcctg cgtcacggcc gcaagcgcac aaggcgggca
      841 gttcagccat gcgccgaaca agttgccggc cggtgtgaca ccctacagcg cgatccacta
      901 cgacggcagc agcgacgatc tgctgactgc gggactcggc aagacggggc tcgctggtgc
      961 gcgcccggcg ccggcggacg tcaacgatcc cgcgcagata cgcaagctcg tgatctggac
     1021 caactaccgc gcggtactcg acatcacggc cgccggcggc ttcggcgtgc tgtacgggcc
     1081 gaacatcgac gtgaatggca acgacacgct gggcgaaggc aaggttgccg gcaccgaata
     1141 catcgcggtg ttcgaccgcg gcgccagcgg ccgcaacgtc acgttgatgg tgcaggtgcc
     1201 cgacgcattc gatccggacc acgcgtgcat cgtcaccggc acctcgagcg gctcgcgcgg
     1261 cgtctacggc gcgatcggtt ccagcggcga atggggcctg aagcgcggct gcgcggtcgc
     1321 atacgcggac aagggcactg gcaatggcgt acacgacctg cagaacaata cggtgaaccg
     1381 gatcaacggc gtgcgcgccg acgccgcggc cgccggcgac gacacggtgt tcaccgcgcc
     1441 gatctccgct ctcgagcggc agcagttcaa cgccgacaca ccgaaccgct tcgcggtcaa
     1501 gcacgcgcat tcgcagctca atcccgagaa ggactggggc cggcacacgc tgacggcgat
     1561 cgagtacgcg ttctatgtgc tcaacgagaa gttcggcacc gagcgcaagg gcaagggcat
     1621 cgcgcgcacg attacgcccg caaacacgat cgtgatcgcc tccagcattt ccaacggcgg
     1681 gggcgcggcg ctgcaggccg ccgagcagga tcgcgctgga ctgatcgacg gggttgcggt
     1741 gactgagccg aacatccagg tgatgcatcc gcagaatccg ccgatccggc gtggcgcagt
     1801 gacctacacc ggcggcagca aaccgctgta cgacttcttc acgttcgaga acctgtatca
     1861 gccgtgcgcg gcgctcgcgc cagcggccgc cgccggcccg ctgtcggtgc tggtcaacgc
     1921 cacacgcgcg gccaaccgtt gccagtcgct cgccgaaaaa ggtctgttat cgagcacgac
     1981 gctcgccgcg caggcgcagg aagcgctcga caagctcatc gcgtatggct ggcagccgga
     2041 gtccacgccg ctgcacgcgt cgcactgggc gctggcgacc ccatcgatca cgatgactta
     2101 cagcaatgcg tacggccgct tctcggtgct cgacaacctg tgcggcttca gcttcgcggc
     2161 gacggacgcg gcaaccggca acgtgctcag caccacgccg gcgctgcttg ccaccgtgtt
     2221 cggcgacggc aacggcgtgc cgccgacgat cggcatcaac atcgtcaaca acctgagccc
     2281 gggtggcgcc aaggccgacg gcctgtcgaa gtcgccgtcg accaatcgcg aggacttcaa
     2341 tctcgacggc gcgctgtgcc agcgcaacct gtggaccggc agcgacacca acgcgcagcg
     2401 tgtgcgcgcc ggcatcgccg aggtgcagca gtcgggctcg ctgcgcggca agccgtcgat
     2461 catcgtgcac ggccgcgcgg acgcgctggt gccggtgaac ttctcgtcgc gcgcgtacgt
     2521 ggcgcagaac cactggcgcg agggcgatgc gaccaacctg cgctacatcg aagtcaccaa
     2581 cgcgcagcac ttcgatgcat tcctcggcct ggccggctac aacagcgcgt tcgtgccgct
     2641 gcacgtgtat ttcgtccgcg cgatggatgc gatgtgggcg cacctgacgc agggcacgcc
     2701 gctgccgccg tcgcaggtcg tgcgcaccac accgcgcggc gcgggcgcgc cgccgatcac
     2761 gggcgccaac gtgccggcga ttgcgtcggc gccgggcgcg gccgatcgcg tcacgttcga
     2821 cggcgatacg ctcgtgattc cggactgatc ggcgtcgttt cgctcacgcg gccggggcgc
     2881 ggtgatgcgc cccggctttt tttgtgcgcg cgaccctccg gtagcatgcc ccacccggta
     2941 cgcgaagact gcccatggcc actggcaacg agccctcgtc gctgtcgaag cttgcgatcg
     3001 atcgttcggc cgcgccgatc cggcgccggc gcccgctgtg gaaacgctgg tgggtctggc
     3061 tgctcgcgct catcgccgcc ggcgccgcgg cggcattcgt gtcgcagcgc gggcgcccgc
     3121 tgccgttcga gatcggcgcg gtggtggccg cgtacccgac ccagagcatc gcgatgctga
     3181 acgcgacggg ccgcgtgtcg gcggcgcggc gggcgtcggt ttcgagcaag gccaccgggc
     3241 ggctcgagtg gctcggcgtg caggaaggcc agcgcgtcca ggagggcgag atcattgcgc
     3301 gcctggagaa ccgcgacgtc gctgcgcagc gcgagcaggc ggccgccaac gtcaaggccg
     3361 cgcaggcgaa cctcgtgcag ggacaggccg aactcgccga cgccgagcag gcgtacaagc
     3421 gcgcgcagga tctgttgcag aagaatttcg tgtccgaatc gtcggtcgac acggcgctcg
     3481 cgcgctacaa caaggcgcgc gcggcgaccg agtcgctgaa ggcgcagatc gcggtcgccg
     3541 aggcca
//
LOCUS       DBNHBY010000139         6465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1496588, whole genome shotgun sequence.
ACCESSION   DBNHBY010000139 DBNHBY010000000
VERSION     DBNHBY010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1496588"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="AC16HB_03295"
     CDS             complement(<1..764)
                     /locus_tag="AC16HB_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR5605844.1"
                     /translation="MTATRLDLLFGVHAHQPAGNFPEVIEQAHRDCYAPFLHTLAEYP
                     QFRCAAHFSGPLLDQLAVQYPRDVAVLREMAARGQIEMFGAGDTEPVLAAIPHRDRIG
                     QLERMALRLERDFGARPRGAWLTERVWEPTVVPALADTGIRYALVDDYHFLCTGKRAG
                     ELDGCFTTEEDGRRLELFAISEELRYRIPFAPAREAVQYLESLARPDGMPAAVYFDDI
                     EKFGVWPETHDWVYGQRWLRDFIEAVLASKVIRTTS"
     gene            complement(761..2479)
                     /locus_tag="AC16HB_03300"
     CDS             complement(761..2479)
                     /locus_tag="AC16HB_03300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR5605845.1"
                     /translation="MSSAGAPGIDLVLVWHMHQPEYRVGAERTPRMPWVYLHALKDYS
                     DMAAHLDAHPQARAVVNFVPILLEQLDDYAQQFAAGNVRDPLLRLLGRPDAGALSAEE
                     RALVLSQCFHANHTHMVAPFPSYRRLYEMARAAEAQNAAEYLSDRFMYDLLTWYHLSW
                     TGEFVRRESPLVKDLMALGSGYSSAHRRALLEVIGNVIARVLVRFRRLEDSERIELST
                     TPYFHPLGPLLVDFGAARQARPDLPLPQAPQYPGGADRLDWHIAAALAEHAHRFGRAP
                     TGLWPAEGAVSDGLLQAAGRARVAWIASGQEVLRNSLRQAGRDPGVGPDCLYRPYRIA
                     GLAPQTLCFFRDDRLSDLIGFVYRSWDGGHAADHFVQELDKIARHGCTDGRRAVVSVI
                     LDGENAWEYYPYNGYYFLSALYDKLADHPHIRMRTYRDWIAEHGTSVLSGVDVLPSLV
                     AGSWVHGDLTTWIGSPDKNAAWDLLVAAKQTYDAAVASGQLTPVEVAAAGRQLGVCES
                     SDWFWWFGDDNPARSVGGFDALYRDNLAQLYALLHRPAPENLAQPVSRGSPSRAGEGA
                     MRRADG"
     gene            complement(2476..3795)
                     /gene="glgC"
                     /locus_tag="AC16HB_03305"
     CDS             complement(2476..3795)
                     /gene="glgC"
                     /locus_tag="AC16HB_03305"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR5605846.1"
                     /translation="MTDAACQHLGSCPYKNDRSGPPAGNLAHDTMAIVLAGGRGSRLG
                     PLSEWRAKPALPFAGKFRIIDFTLSNCVNSGIRRIGICTQYKAQSLIRHVQRGWSIFD
                     GRFNEFIELLPAQQRVTTHWYAGTADAIYQNIDILRVQEPRFVLVLAGDHVYKMDYSK
                     MLGEHVARNADMTIACLEVPVADARPFGVMQADAEERIVGFQEKPAHPRPLPDRPDTA
                     LVSMGVYCFNTPFLYEQLIRDAGMRTSSHDFGKDIIPYSIERGYRVFAHRFAASCVNM
                     VGDRPYWRDVGTIDAYWEANLDLVHVTPELNLYDEAWPIWTNQEQLPPAKFVFDDDDR
                     RGIAIDSIVSGGCIISGSTVRRSLLFSNVHVHSYCTIEDSVLLPDVDVARHVVLKRVV
                     VEKGCRLPEGLQAGVDHHADRHRFRVTDHGITLITPQMLGQEIHQVP"
     gene            3861..6044
                     /gene="glgB"
                     /locus_tag="AC16HB_03310"
     CDS             3861..6044
                     /gene="glgB"
                     /locus_tag="AC16HB_03310"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR5605847.1"
                     /translation="MNDHSWAGAPELRERLFAARLHEPLALLGLHGDPGAGWTYRCYL
                     PYARSVALALADGWAAMTAAGEGLFVWNGAAAPPRPYRLRAVAAGAEREFIDPYGFAP
                     TIGDFDLHLFNSGRLLEGYRILGANHIDLDGVRGVRFAVWAPNAERVSVVGDFNRWDG
                     RRHPLQSRGASGVWELFVPGLAAGVLYKFEIRNRASGHVLLKTDLYGRSFELRPGTAA
                     RVASAPAHAWGDGEWLARRAGWDWQHAPISIYEVHAGSWRRHADGGLLDYRALAQTLV
                     PYASELGFTHVELLPITEHPLDESWGYQTTGYFAPTCRHGDADDLKFFVDACHRAGLG
                     VILDWVPGHFPSDDFALAHFDGTALYEHEDPRLKIHPDWSTYVFNYGRAEVRSFLLSS
                     AHYWLDEFHFDGLRVDAVASMLYLDYSRREGEWLPNKYGGRENLEAIGFLRDLNVMVH
                     ERFAGAVTIAEESTAWPMVSRPTHVGGLGFSMKWNMGWMHDTLDYLRRDPAHRRYHHE
                     LLSFGQLYAYSENFVLPLSHDEVVHGKGSLRAKMPGDEWQRFATLRLLATYQATCPGK
                     KLVFMGNEIGQAQEWNASAQLDWESLREEPAAGLQRLVATLNRLYRDTPALHELDFES
                     DGFRWVDCHDADHSILSYLRRARDGRSVLIVLNFTPVPRHGYRLGVPGRGRYIEWLNS
                     DSRHFGGGDVGNAGGVDAEPAPCMGQAWSIVLTLPPLAGLLIECP"
     gene            6079..>6465
                     /locus_tag="AC16HB_03315"
     CDS             6079..>6465
                     /locus_tag="AC16HB_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR5605848.1"
                     /translation="MTLRVLFAASECVPLVKTGGLADVAGALPAALRRLDLDVRVLLP
                     GYPPVLEALRGRRPLAFIEPFADLPAADLLAAELPDGVPLYVIDCPPLFDRAGGLYQD
                     GGKDWPDNALRFALLGRIAAALANDRG"
BASE COUNT         1018 a         2160 c         2189 g         1098 t
ORIGIN      
        1 aagctcgtcg tgcggatcac cttcgatgcg agcaccgctt cgatgaagtc acgcagccag
       61 cgctgcccgt agacccagtc gtgcgtctcg ggccagacgc cgaacttctc gatgtcgtcg
      121 aagtacacgg ccgccggcat gccatccggg cgcgccaagg actccaggta ctgcaccgct
      181 tcgcgcgccg gtgcaaacgg aatccggtag cgcagttcct cggagatcgc gaacagttcg
      241 agtcgccggc cgtcctcctc ggtcgtgaaa cagccgtcga gttcgcccgc gcgcttgccg
      301 gtgcagagga agtgataatc gtcgaccagc gcgtaccgaa tgccggtgtc ggcgagcgcc
      361 ggcacgacgg tcggctccca cacgcgctcc gtcagccacg cgccgcgcgg gcgcgcgccg
      421 aaatcgcgtt cgaggcgcag cgccatgcgt tcgagctggc cgatgcggtc gcgatgcggg
      481 atcgcggcca gcaccggctc ggtgtcgccg gcgccgaaca tttcgatctg cccgcgggcc
      541 gccatctcgc gcagcacggc gacgtcgcgc ggatactgca cggccagctg atcgagcagc
      601 ggcccactga aatgcgcggc gcaacggaac tgcggatact ccgccagcgt gtgcagaaac
      661 ggcgcgtagc agtcgcggtg cgcctgttcg atcacttcgg ggaagttccc ggccggctgg
      721 tgcgcgtgca cgccgaacag cagatcgagt cgtgtggccg tcatccgtcg gccctgcgca
      781 ttgcgccttc gcccgcgcgc gacgggctgc cgcggctgac gggttgcgca aggttctccg
      841 gagctggccg gtgcagcagc gcgtacaact gcgcgagatt gtcgcgatac agcgcgtcga
      901 agccgccgac cgatcgcgct ggattgtcgt cgccgaacca ccagaaccag tccgagctct
      961 cgcacactcc gagctgtcgc cccgccgcgg cgacctcgac cggcgtcagc tgaccgctcg
     1021 cgactgcggc gtcgtaagtc tgcttggccg cgacgagcag gtcccacgcc gcattcttgt
     1081 ccggcgaacc gatccacgtc gtcagatcgc cgtggaccca gctgcctgcg accagcgacg
     1141 gcaacacgtc gacgccgctg agcacgctcg taccgtgttc ggcaatccag tcgcggtagg
     1201 ttcgcatccg gatgtgcgga tggtcggcga gcttgtcgta cagagctgag aggaagtaat
     1261 agccgttgta tgggtagtac tcccacgcgt tttcgccgtc gaggatcacg ctgacgacgg
     1321 cgcgccggcc gtcggtgcag ccgtgccggg cgatcttgtc cagctcctgc acgaagtgat
     1381 ccgccgcgtg gccgccgtcc cagctgcggt agacgaaacc gatcagatcg gacagccgat
     1441 cgtcgcggaa gaaacaaagc gtttgcggcg cgagtccggc gatgcgatac ggtcgataca
     1501 gacagtccgg cccgacgccg gggtcgcgtc cggcctgtcg caggctgttg cgcagcacct
     1561 cttgtcccga ggcgatccat gccacgcgcg cgcgcccggc cgcctggagc agcccgtcgc
     1621 tgaccgcgcc ttccgcgggc cacagtccgg tcggtgcacg gccgaagcgg tgcgcatgct
     1681 cggcgagggc ggccgcgatg tgccagtcga gccggtcggc accccccgga tactgcggcg
     1741 cctgcggcag cggcagatcg ggccgagcct ggcgcgccgc gccgaagtcg accagcagcg
     1801 gccccagcgg atgaaagtag ggcgtcgtcg acaactcgat gcgctccgaa tcctccagcc
     1861 gccggaatcg gaccagtacc cgcgcgatca cgttgccgat cacctcgagc agggcccgtc
     1921 tgtgtgcgga cgagtacccg ctgccgagcg ccatcagatc cttgaccagc ggagattcgc
     1981 gccgcacgaa ttcgccggtc cacgacaggt ggtaccaggt gagcaggtcg tacatgaaac
     2041 ggtcggacag atactcggcg gcgttctgcg cctcggccgc gcgcgccatt tcgtacagcc
     2101 gtcgatacga cggaaacggt gcgaccatgt gcgtgtgatt cgcgtggaaa cactggctga
     2161 gcaccagtgc gcgttcctcc gcggagagcg cgccagcgtc cggccggccg agcaggcgca
     2221 gcaatggatc gcgcacgttg cccgcggcga actgctgcgc gtagtcgtct aactgctcga
     2281 gcaggatcgg cacgaagttg acgaccgcgc gtgcttgcgg gtgcgcgtcc aggtgcgcgg
     2341 ccatgtccga gtagtccttc agggcatgca gatacaccca cggcatgcgc ggcgtacgct
     2401 cggcgccgac gcgatactcc ggctggtgca tgtgccacac cagtaccagg tcgatgcctg
     2461 gagcacccgc agaactcatg gcacctggtg aatttcctgc cccagcatct gcggcgtgat
     2521 cagcgtgatt ccgtgatcgg tcacgcggaa gcggtgccgg tcggcgtggt ggtcgacgcc
     2581 ggcctgcagc ccttcgggca ggcggcaacc cttttcgacc accacgcgct tgagcaccac
     2641 gtggcgcgcg acatcgacat ccggcagcag caccgagtcc tcgatcgtgc agtagctgtg
     2701 cacgtgcacg ttggaaaaca gcagcgagcg gcgcaccgtc gagccgctga tgatgcagcc
     2761 accggagacg atcgaatcga tcgcgatgcc acgccggtcg tcgtcgtcga acacgaactt
     2821 ggcgggaggc agctgttcct ggttggtcca gatcggccat gcctcgtcgt agagattcag
     2881 ctcgggcgtg acatgcacga ggtccagatt ggcctcccag taggcgtcga tcgtgccgac
     2941 atcgcgccag tacgggcggt cgccgaccat gttgacgcag ctggcagcga agcggtgggc
     3001 aaacacgcgg tagccgcgct cgatcgagta gggaatgatg tccttgccga agtcgtgcga
     3061 tgacgtacgc atgccggcat cgcggatcag ttgctcgtac aggaacggcg tgttgaagca
     3121 atacacgccc atgctgacga gtgcggtatc gggacggtcg ggcagcggcc gcggatgcgc
     3181 cggcttctcc tggaaaccga cgatgcgctc ctcggcgtcc gcctgcatca cgccgaaagg
     3241 ccgtgcatcc gccaccggca cctcgaggca cgcgatcgtc atgtcggcgt tgcgcgccac
     3301 gtgctcgccg agcatcttcg agtagtccat cttgtagacg tgatcgcccg ccagcacgag
     3361 cacgaagcgc ggttcctgca cgcgcaggat gtcgatgttc tgatagatcg catcggcggt
     3421 gcccgcatac caatgcgtgg tcacgcgctg ctgcgccggc agcagttcga tgaactcgtt
     3481 gaagcggccg tcgaagatgg accagccgcg ctgcacgtgg cggatcaggc tctgcgcctt
     3541 gtactgcgtg cagatgccga tgcggcggat gccggagttc acgcagttcg acagcgtgaa
     3601 gtcgatgatg cggaacttgc cggcgaacgg cagcgccggc ttggcgcgcc attcgctcag
     3661 tggtccgagc cggctgccgc gcccgccggc cagcacgatc gccatcgtgt cgtgtgccag
     3721 gttgcccgcg ggcgggcccg acctgtcgtt cttgtacgga caagagccga ggtgctggca
     3781 cgcggcgtcg gtcatctgct ctcccatcgc atccgtgtcg caccggacgc cgcgcgcgcg
     3841 gctcttatac tgcctcggcc atgaatgacc actcgtgggc cggcgcgccc gaactgcgcg
     3901 aacgcctctt cgcggcccgg ttgcacgagc ccctcgcgct gctcggcctg cacggcgacc
     3961 ctggagccgg ctggacctac cgttgttatc ttccgtatgc ccgcagcgta gcgctcgcgc
     4021 tcgccgacgg ctgggccgcg atgaccgcgg ccggcgaagg cctgttcgtc tggaacggcg
     4081 ccgcggcccc gccgcggccg taccgattgc gcgccgtggc agcgggtgca gaacgcgaat
     4141 tcattgaccc gtatggcttt gcgccgacca tcggcgactt cgacctgcat ctgttcaact
     4201 ccgggcgcct gcttgaaggc taccgcatct tgggagcgaa ccacattgac ctcgatgggg
     4261 ttcgcggcgt gcgctttgcc gtctgggcgc cgaatgccga gcgcgtatcg gtcgtcggcg
     4321 atttcaatcg ctgggacgga cggcggcatc cgctgcaatc gcgtggcgca agcggcgtgt
     4381 gggaactgtt cgtgcccgga ctcgctgcag gcgtcctgta caagttcgag atccggaatc
     4441 gcgcgtccgg gcacgtcctg cttaaaacgg atctgtacgg acgctcgttc gaactgcgac
     4501 ccggaacggc ggcgcgcgtg gcgtcggcgc cggcccatgc atggggcgac ggcgaatggc
     4561 tcgcgcgccg cgcaggctgg gactggcagc acgcgccgat cagcatctac gaggtacacg
     4621 ccggatcgtg gcgccgccat gccgatggtg gtttgctcga ctatcgcgcg ctggcacaga
     4681 cgctggtgcc gtatgcgtcc gaactgggct tcacgcacgt ggagttgctg ccgatcaccg
     4741 aacatccgct cgacgaatcc tggggctacc agacgaccgg ctacttcgcg ccgacctgtc
     4801 gccacggcga cgccgacgat ctgaagttct tcgtcgatgc gtgtcatcgc gccggcctcg
     4861 gcgtgatcct cgactgggtg cccggccatt tcccgagcga cgacttcgcg ctcgcgcact
     4921 tcgacggcac ggcgctgtac gagcatgagg acccgcgcct gaagatccac cccgactggt
     4981 ccacgtacgt gttcaactac gggcgcgccg aggtgcggtc gttcctgctg tcgagtgcgc
     5041 attactggct cgacgagttt catttcgatg gcttgcgcgt tgacgcagtc gcttcgatgc
     5101 tgtacctcga ctattcgcgc cgcgaaggcg aatggctgcc gaacaagtac ggcggccgcg
     5161 aaaacctgga ggcgatcggc ttcctgcgcg acctgaacgt gatggtgcac gagcgcttcg
     5221 cgggcgccgt gaccatcgcc gaggagtcga ccgcgtggcc gatggtgtcg cggccgacgc
     5281 atgtcggcgg gctcggcttc tcgatgaagt ggaacatggg ctggatgcac gacacgctcg
     5341 actatctgcg gcgggatccg gcgcatcgcc gctaccacca cgagttgctg agcttcggcc
     5401 agctgtacgc gtacagcgag aacttcgtgc tgccgctttc gcatgacgag gtcgtgcatg
     5461 gcaagggctc gctgcgcgcg aagatgcccg gcgacgaatg gcagcgtttt gccaccctgc
     5521 gactgctcgc gacctaccag gcgacctgcc ccggcaagaa gctcgtgttc atgggtaacg
     5581 aaatcggcca ggcgcaggaa tggaatgcgt ccgcgcaact ggactgggag tcgctgcgcg
     5641 aagagcctgc ggctgggctg cagcgactgg tcgccacgct caatcgcctg taccgcgata
     5701 cgccggcgtt gcacgaactg gatttcgagt ccgacggttt ccgctgggtc gactgccacg
     5761 atgcggacca ctccatcttg agctacctgc gccgcgcgcg cgatggccgc agcgtgctga
     5821 tcgtgctgaa tttcacgccg gtgccgcggc acggctaccg cctcggcgtt cccggccgcg
     5881 gccgctatat cgagtggctg aacagcgact cgcgccactt cggcggcggc gatgtcggca
     5941 acgcgggcgg cgtcgatgcg gagcccgcgc cgtgcatggg ccaggcatgg tcgatcgtgc
     6001 tcacgctgcc gccgctggcg gggctgctga tcgaatgccc gtaggacgtc gtccttcgcc
     6061 acgaaccgaa gcctcgccat gacactgcgc gtgctgtttg cggcatccga gtgcgtgccg
     6121 ctggtcaaga ccggcggcct ggccgacgtg gcgggcgcgt tgccggcggc gctgcgccgg
     6181 ctggatctgg atgtgcgcgt gctgctgccg ggctatccgc cggtgctgga ggccctgcgc
     6241 ggacgccgcc ctctggcgtt catcgaacct ttcgccgatc tgccggcggc cgatctgctc
     6301 gctgccgaac tccccgacgg cgtgccgctg tacgtgatcg attgtccgcc gttgttcgac
     6361 cgtgcgggcg gcttgtatca ggacggcggc aaggactggc ccgacaacgc attgcggttt
     6421 gctctgctcg gccggatcgc cgcggcgctg gcgaacgacc gcggt
//
LOCUS       DBNHBY010000140         6035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_955344, whole genome shotgun sequence.
ACCESSION   DBNHBY010000140 DBNHBY010000000
VERSION     DBNHBY010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_955344"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC16HB_03320"
     CDS             complement(<1..509)
                     /locus_tag="AC16HB_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HIR5605849.1"
                     /translation="MARITASVYTSHVPAIGAALDLGKTHEPYWQPLFAGYEVSKRWM
                     LANRPDVIFLVYNDHATAFSLEIIPTFAIGTAAEFKVADEGWGPRPVPNVIGHPELAS
                     HIAQSVIQDDFDLTIVNRMDVDHGLTVPLSLMCGQPQAWPCRVIPFAVNVVQYPVPSG
                     RRCFQLGQA"
     gene            complement(509..904)
                     /gene="ligA"
                     /locus_tag="AC16HB_03325"
     CDS             complement(509..904)
                     /gene="ligA"
                     /locus_tag="AC16HB_03325"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HIR5605850.1"
                     /translation="MALEKPYLDIPGTTIFDADQARKGYWLNQFCVSLMKAENRARFK
                     ANERAYLAEWPMTEAQKQAVLDRDLNRCIALGGNIYFLARIGATDGKSFQQMAGSMTG
                     MSEEEYRKMMVSGGRPIEGNRYLHEWERR"
     gene            complement(906..1820)
                     /locus_tag="AC16HB_03330"
     CDS             complement(906..1820)
                     /locus_tag="AC16HB_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5605851.1"
                     /translation="MAFEKTPGWLDWYAAPSRPMFRLPTGSVDAHCHVFGPGVEFPFA
                     PERKYTPCDASKHQLFALRDHLGFARNVIVQATCHGADNRALVDACLASGGKARGVAT
                     VKRDVTDKELEALHAAGIRGVRFNFVRRLVDFTPRDELMEIAARIAPLRWHVVVYFEA
                     QDLPELWPFFTSLPTVVVVDHMGRPDVSRPVDGPEFELFVRLLREHENIWSKVSCPER
                     LSKSGPPALHGQRSAYRDVVPFARRVVETFPDRVLWGTDWPHPNLKDHMPDDGLLVDF
                     IPHIATTPVLQKKLLVDNPMRLYWPEEV"
     gene            complement(1822..2748)
                     /locus_tag="AC16HB_03335"
     CDS             complement(1822..2748)
                     /locus_tag="AC16HB_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5605852.1"
                     /translation="MTIPSDSRQWRVGLVGYGEVGRILAEDLRLQGVMVRAYDIKLDG
                     GQGSAIRQHAAQHGVVLADSHVALAADADFIISAVTASQAVPVAEACAPAVRRGVFFL
                     DFNSASPGAKVRAGAIIDGVGGRYVEGAVMTSVPPKRIKVPLLLGGPSAKALEPLVNA
                     LGFAASVSSDKLGIASATKMCRSVMIKGLEAMVIESFTAARAYGVEDAVVASLYETFP
                     GIDWERQAAYLFQRVIEHGRRRSEEVREVAQTVREIGLEPWSAAGTAERQAWVADLAD
                     AGWFGQRGTPAFARSADWRTEADRILAYLGKA"
     gene            complement(2753..3718)
                     /locus_tag="AC16HB_03340"
     CDS             complement(2753..3718)
                     /locus_tag="AC16HB_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5605853.1"
                     /translation="MDRRQFLLAGAAWALLAPSARAQAFPSKPIHIIVGYTAGGAVDA
                     IARVVGTQLSTSLGQPVIIDNKPGAATNVAVKALIDSPPDGHTLMLTANAIAANMALF
                     QPAPYDLERDVAPVAFVGRVPVVIAANAASPITTLTKLVEAAKAKPGSLDYATPGNGS
                     TPHLAVELFARAAGIKLSHVPYRGGAQALTDVIGGQVPLVALNALEVLPHAKSGKLRV
                     VAVLSAGRSSVLPDVPTIAESGYPGFEASVWYGLIAPAATPKAIVTQLNAATQKALQS
                     AEVRDRLTSAGGEVAPGTPEAFGAYLRAERLRYDKLIREAQIRPD"
     gene            complement(3730..4413)
                     /gene="ligK"
                     /locus_tag="AC16HB_03345"
     CDS             complement(3730..4413)
                     /gene="ligK"
                     /locus_tag="AC16HB_03345"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HIR5605854.1"
                     /translation="MYELGVVKRTITRADAAAVGQLARFGVATIHEAMGRVGLMKPYL
                     RPIYPGAQLAGTAVTVLMHPGDNWMLHVVAEQIREGDVVVAAVTADCTDGYFGELLAT
                     SFRARGARGLVIDAGVRDVKDLTAMQFPVFSRAISAKGTIKATLGSVNIPVVCAGAPV
                     EPGDVVVGDDDGVVVVPAALARKTADAAAAREANEADKRARLAAGVLGLDMYKMREPL
                     EKAGLKYID"
     gene            complement(4416..5444)
                     /locus_tag="AC16HB_03350"
     CDS             complement(4416..5444)
                     /locus_tag="AC16HB_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5605855.1"
                     /translation="MIIDCHGHYTTAPQALEQWRNRQVAGIKDPAAMPKAAELSISDD
                     ELRESIEINQLAKMKERGADLTIFSPRASFMAHHVGDFDVSSTWAAICNELCFRVAQL
                     FPDHFIGCAMLPQSPGASPATCIPELEKCVREYGFVAINLNPDPSGGHWNSPPLTDRH
                     WYPIYEKMVEYEIPAMIHVSTSCNACFHTTGAHYINADTTAVMQLIQGDLFKDFPTLK
                     FVIPHGGGAAPYHWGRYRGLAQELRKPLLKDHLLRNIFFDTCVYHQPGIDLLTKVIPV
                     DNILFASEMIGAVRGIDPETGHHYDDTRRYIEAATILGVADRFKIYEGNARRVYPRLD
                     AALKLRGK"
     gene            complement(5476..>6035)
                     /locus_tag="AC16HB_03355"
     CDS             complement(5476..>6035)
                     /locus_tag="AC16HB_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5605856.1"
                     /translation="RVQAGEPFDVVVLAADAIDALVASGRVVAGSEVDLVRSGVAIAV
                     RTGIPRPDIGSANALERAVLSARTVGYSTGPSGVALTKLLERWGIAEAIRGRLVQAAP
                     GVPVGQLVARGEVELGFQQLSEMINVDGIDVIGPMPPGLEILTVFAGGVCTASTRPEA
                     ARALLDFMRSPAAAEAKRRLGMEPA"
BASE COUNT          961 a         2068 c         2000 g         1006 t
ORIGIN      
        1 atcgcctgcc cgagctggaa gcagcgccgt cccgacggca ccgggtattg caccacgttg
       61 acggcgaacg gaatcactcg gcacggccag gcctgcggct gaccgcacat cagcgacagc
      121 ggcacggtga gtccgtggtc gacgtccatc cggttgacga tcgtcagatc gaaatcgtcc
      181 tggatcaccg actgcgcgat gtgcgacgcc agttccggat gcccgatcac gttcggcacc
      241 ggacgtggac cccagccctc gtcggcgacc ttgaactccg ccgccgtccc gatcgcaaag
      301 gtcggaatga tctcgaggct gaacgcggtc gcgtgatcgt tgtagacgag gaagatcacg
      361 tcgggccggt tcgccagcat ccagcgtttg gagacttcgt agcccgcgaa cagcggctgc
      421 cagtacggct cgtgcgtctt gccgaggtcg agcgccgcgc cgatcgccgg cacgtgcgac
      481 gtgtagacgc tggcggtgat ccgtgccatt accgcctctc ccactcgtgc agatagcggt
      541 tgccctcgat cggacggccg ccgctcacca tcatcttccg gtattcctcc tccgacatgc
      601 cggtcatgct gccagccatc tgctggaagc tcttgccgtc ggtcgccccg atccgcgcca
      661 ggaagtagat gtttccgccc agagcgatac agcggttcag gtcacgatcg agcacggcct
      721 gcttctgtgc ctcggtcatc ggccactcgg cgaggtatgc gcgctcgttt gccttgaagc
      781 gcgcgcggtt ctcggccttc atcagcgaca cgcagaactg gttgagccag taacccttgc
      841 gcgcctggtc cgcatcgaag atggtggtgc ccggaatgtc gaggtacggc ttctcgaggg
      901 ccatgtcaga cctcctccgg ccaatacagg cgcatcggat tgtcgaccag cagcttcttc
      961 tgcagtaccg gcgtggtcgc gatatgcgga atgaagtcga ccagcagccc gtcgtcgggc
     1021 atgtgatcct tcaggttggg gtgcggccag tcggtccccc acagcacgcg atccgggaag
     1081 gtctcgacga cacggcgcgc gaacggcacg acgtcgcgat aggcgctgcg ctggccatgc
     1141 aacgccggtg gcccgctctt cgacaggcgt tccgggcagc tcaccttcga ccagatgttc
     1201 tcgtgctcgc gcagcagtcg cacgaacagt tcgaattccg gcccatcgac cggccggctc
     1261 acgtcgggcc gacccatgtg gtcgacgacc accacggtcg gcaacgacgt gaagaagggc
     1321 cacaactccg gcaggtcctg cgcctcgaaa tagacgacga cgtgccagcg gagtggcgca
     1381 atgcgcgcgg cgatctccat caactcgtcg cggggcgtga agtcgaccag ccgcctgacg
     1441 aagttgaagc gcacgccgcg tatgcccgcg gcgtgcagtg cctcgagttc cttgtcggtc
     1501 acgtcgcgct tgaccgtcgc cacgccccgc gctttgccgc cggaggccag gcaggcgtcg
     1561 acgagcgcgc ggttgtcggc gccatggcag gtcgcctgga cgatcacgtt gcgcgcgaaa
     1621 ccgaggtgat cgcgcagcgc gaacagctga tgctttgaag cgtcgcaggg cgtgtacttg
     1681 cgctccggcg cgaacgggaa ttcaacgccg ggcccgaata cgtggcagtg cgcgtcgacg
     1741 ctgcccgtcg gcagcctgaa catcggccgc gacggcgcgg cgtaccagtc gagccagcca
     1801 ggcgtcttct cgaatgccat gtcatgcctt cccgagataa gcgaggatgc ggtccgcctc
     1861 ggtgcgccag tccgcgctgc gcgcaaaggc cggcgttccg cgctgaccga accagcccgc
     1921 gtccgcgagg tcggcaaccc aggcctggcg ctccgcggtg ccagcagcgg accaaggctc
     1981 gaggccgatc tcgcgcacgg tctgtgcgac ctcgcgcacc tcctcgctgc ggcggcgacc
     2041 gtgctcgatc acgcgctgga agaggtaggc ggcctgccgc tcccagtcga tgcccgggaa
     2101 ggtctcgtat agcgacgcca ccacggcgtc ctcgacgcca tacgcgcgtg ccgcagtgaa
     2161 gctctcgatc accatcgcct cgaggccctt gatcatcacg ctgcggcaca tcttcgtcgc
     2221 ggaggcgatg ccgagcttgt cgctcgatac gctggcggcg aagccgagcg cgttgaccaa
     2281 cggttccagc gccttcgcgc tcgggccgcc gagcaacagc ggcaccttga tgcgtttcgg
     2341 cgggaccgag gtcatcacgg cgccctcgac gtaacggccg ccgacgccgt cgatgatcgc
     2401 gccggcgcgg accttcgcgc cgggcgaggc ggagttgaag tcgaggaaga acacgccgcg
     2461 tcgcaccgcc ggcgcgcagg cctcggccac cggcaccgcc tgactggcgg tgactgccga
     2521 gatgatgaag tccgcgtcgg ccgcaagggc gacgtgcgag tcggcgagca cgacgccgtg
     2581 ttgcgccgcg tgctggcgga tcgcgctgcc ttgcccgccg tcgagcttga tgtcgtacgc
     2641 cctgaccatg acgccctgca ggcgcaggtc ctcggcgagg atgcggccga cctcgccgta
     2701 gccgacgagg ccgacgcgcc actgccggga atcggacgga atcgtcattg cttcagtccg
     2761 gcctgatctg cgcttcgcgg atcagcttgt cgtagcgcag gcgctcggcg cgcaggtagg
     2821 cgccgaaggc ttccggcgtt ccgggcgcga cttcgccgcc ggccgacgtg agccggtcgc
     2881 gcacctcggc tgactgcagc gccttctgcg tggcggcgtt gagttgagtc acgatcgcct
     2941 tcggtgtcgc ggccggtgcg atcaatccgt accagaccga ggcctcgaag ccggggtagc
     3001 ccgattcggc gatggtcggc acgtcgggca agacactgct gcgtccggcg ctgagcacgg
     3061 ccaccacgcg caacttgccg ctcttcgcat gcggcagcac ctcgagcgcg ttcagcgcaa
     3121 cgagcggcac ctgcccgccg atcacgtcgg tcagcgcctg cgcgccgccg cgatacggca
     3181 cgtgcgagag cttgatgccg gccgcgcgcg cgaacaactc caccgcgagg tgcggcgtcg
     3241 atccgttgcc gggggtcgcg tagtcgagcg agccgggctt ggcctttgcg gcctcgacga
     3301 gcttggtcag ggtcgtgatc ggcgatgcgg cgttcgcggc gatcaccacc ggcacgcgcc
     3361 cgacgaacgc caccggcgcg acatcgcgtt cgaggtcgta cggcgccggc tggaacagcg
     3421 ccatgttcgc tgcaatcgca ttggccgtca gcatcagcgt gtggccatcg ggcgggctgt
     3481 cgatcagcgc cttcaccgcg acgttggtcg ccgcgccagg cttgttgtcg atgatcacgg
     3541 gttgtccgag gctcgtcgag agttgcgtgc cgaccacgcg ggcgatcgca tcgacggcgc
     3601 cgcccgccgt gtagccgacg atgatgtgga tcggcttgct cggaaacgcc tgtgccctgg
     3661 cagacggagc gagcaacgcc catgcagcgc cagcaagcag gaactgtctt cggtccacga
     3721 atcgccccct cagtcgatgt atttaaggcc cgccttctcc agcggctcgc gcatcttgta
     3781 catgtcgagc cccagcacgc cggcggcgag cctggcgcgc ttgtcggcct cgttggcctc
     3841 gcgcgctgcc gctgcgtcgg ctgtcttccg ggcgagtgcg gccggcacga cgacgacgcc
     3901 atcatcgtcg ccgacgacca cgtcacccgg ttccaccggc gcgcccgcgc acacgacggg
     3961 gatgttgacg gaccccagcg tggccttgat cgtgcccttg gcgctgatgg cgcgggaaaa
     4021 caccggaaac tgcatcgcgg tcaggtcctt cacgtcgcgc acgccggcat cgatgacgag
     4081 cccccgggcg ccgcgcgcgc ggaagctggt ggccagcagt tcgccgaagt agccgtcggt
     4141 gcagtccgcc gtcacggccg ccaccacgac atcgccctcg cggatctgct ccgcgacgac
     4201 atgcagcatc cagttgtcac cgggatgcat cagcaccgtg accgcggtcc cggcgagttg
     4261 tgcgcccggg tagatcggcc gcaggtaagg cttcatcagg cccacccggc ccatcgcttc
     4321 gtggatggtc gccacgccga agcgggccag ctggccgacc gcggccgcgt cggcacgcgt
     4381 gatggtgcgc ttcacgactc cgagttcgta catgatcact tccctctgag cttcagtgcc
     4441 gcatccaggc gcggatagac gcgccgcgcg ttgccctcgt agatcttgaa gcggtccgcg
     4501 acgccgagga tcgtcgcggc ctcgatgtac cgccgggtgt cgtcgtagtg atgcccggtc
     4561 tccggatcga tgccgcgcac cgccccgatc atctcgctgg cgaacaggat gttgtccacg
     4621 ggaatcacct tcgtcagcag gtcgatgccc ggctggtgat agacgcaggt gtcgaagaag
     4681 atgttccgaa gcagatggtc cttcagcagc ggcttcctca actcctgcgc cagtccgcgg
     4741 tagcggcccc agtggtaggg tgcagcgccg ccgccgtgcg ggatcacgaa tttcagcgtc
     4801 ggaaaatcct tgaacaggtc gccctggatc agctgcatca ccgccgtcgt gtcggcattg
     4861 atgtaatgcg cgccagtggt atggaagcac gcattgcacg acgtgctgac gtggatcatc
     4921 gccgggatct cgtactcgac catcttctcg tagatcgggt accagtggcg gtcggtcagc
     4981 ggcggcgaat tccagtggcc gccggacgga tcgggattca gattgatcgc gacaaagccg
     5041 tactccctga cgcacttctc gagttcggga atacaggtcg caggactggc gcccggcgac
     5101 tgcggcagca tcgcgcagcc gatgaagtga tcaggaaaga gctgcgcgac gcggaagcac
     5161 agctcgttgc agatcgcggc ccacgtcgag ctgacgtcga agtcgccgac gtggtgcgcc
     5221 atgaagctgg cgcgcgggga gaagatcgtc agatcggctc cgcgttcctt catcttcgcg
     5281 agctgattga tttcgatcga ctcgcgcagc tcgtcgtcac tgatcgaaag ttcggcggcc
     5341 ttcggcatcg ccgccgggtc cttgatgccc gcgacctgac gattgcgcca ctgctccagc
     5401 gcctggggcg cggtggtgta gtggccgtga cagtcgatga tcatcgatgc gctccttgtc
     5461 tttcgcgtgc cgccgtcacg ccggctccat gcccaggcgc cgcttcgctt cggcggcagc
     5521 gggcgagcgc atgaagtcga gcagggcgcg cgcggcctcc ggtcgagtcg acgcggtgca
     5581 tacaccgccc gcaaagacgg tgaggatctc gaggccgggc ggcatcgggc cgatgacgtc
     5641 gatgccgtcg acgttgatca tttcactcaa ctgctggaag cccagttcga cttcgccgcg
     5701 cgcgaccagt tgccccaccg gcacgccggg cgccgcctgg acgagacgac cgcgaatcgc
     5761 ttctgcgatg ccccaccgtt ctaagagctt cgtcaacgca acgccgctgg gcccggtcga
     5821 gtacccgacc gtgcgcgccg acagcaccgc gcgctcgagc gcatttgccg aaccgatgtc
     5881 cggccgcggg atgcctgtgc gcaccgcaat cgccacaccc gaacgcacga ggtcgacttc
     5941 gctgcccgcg accacccgtc cggaggcgac cagcgcgtcg atcgcgtcgg ccgcgagtac
     6001 gaccacgtcg aacggctcac cggcctgaac ccgtc
//
LOCUS       DBNHBY010000141         3437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1834844, whole genome shotgun sequence.
ACCESSION   DBNHBY010000141 DBNHBY010000000
VERSION     DBNHBY010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1834844"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..569)
                     /gene="lolA"
                     /locus_tag="AC16HB_03360"
     CDS             complement(30..569)
                     /gene="lolA"
                     /locus_tag="AC16HB_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR5605857.1"
                     /translation="MRSFLSDTKSARGEFSQRVSAGKKPPVSSTGRFEFERPGRFRWS
                     YDKPYEQVIVSDGQRLFVYDKDLNQVTVKKIGAALPASPASILFGTNDFEREFDVRDD
                     GERDGLAWIVAKPRTSDSQFDAIRIGFKGGVPGAMQIADTFGQTTVLAFKSFERNPKL
                     DAGLFQFVPPKGADVLEDR"
     gene            complement(639..2945)
                     /locus_tag="AC16HB_03365"
     CDS             complement(639..2945)
                     /locus_tag="AC16HB_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR5605858.1"
                     /translation="MESTIQPGGVHSRRRSAAAPEPPSRGARLLAEARWIVGAAAVIA
                     LASILITYSRADPGFSHSTSGALIHNVGGRVGAWVADVLLLVFGFSAWLWVVGGAAWA
                     LRGFRRLHAQYRDHGLPDWLQGVGFVVLLASCSTLEALRLYTLGVQLPAAPGGVSGGL
                     LAGALKGALGFTGTSVLLIVAIALGASLFFDFSWLALAERVGGWFESIAQRTRAAREA
                     KEDRAIGEAVARSREQMIEQERERIDEAPPVHIERAEARVKPSERKQKEKQVPLFAEL
                     ADGSLPALDLLDEPPANVETISTETLEFTSRLIEKKLKDFGVEVAVVAAYPGPVITRY
                     EIEPAVGVKGSQIVNLAKDLARALSLVSIRVVETIPGKNLMGLELPNPKRQIVKLSEI
                     LGSQTYNDSHSLLTLGLGKDIGGKPVVIDLARMPHLLVAGTTGSGKSVGINAMILSVL
                     YKADPSQVRLLLVDPKMLELSVYEGIPHLLAPVVTDMKEAAHALNWCVAEMERRYRVM
                     SKVGVRGLTTYNQKIAEAKAKGETIPNPFTLTPEAPEPLDTLPMIVLVIDELADLMLV
                     IGKKVEEPIIRLAQKARAAGIHLILATQRPSVDVITGLIKANIASRIAYQVASRVDSR
                     TILDQMGAETLLGQGDMLYLAGGTGLPTRVHGAFVSDAEVHRVVEMLKARGAPQYVEG
                     ILDAPEAPLPGMEGEGQPGGEADPLYDQAVEIVLKNRRASISLVQRHLRIGYNRAARL
                     LEDMEKAGMVSAMQPNGNREILVPHREP"
     gene            3113..>3437
                     /locus_tag="AC16HB_03370"
     CDS             3113..>3437
                     /locus_tag="AC16HB_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605859.1"
                     /translation="MATATSADPDRAGFEWRAERFRHEFFRFFNFGTVAAVFFICLAV
                     AFTRWLPTLYFDAPAIWALDGIRYLRQTLISGLCALAAIALVQALARAFDWSQRRTYA
                     VGVIAI"
BASE COUNT          515 a         1218 c         1125 g          579 t
ORIGIN      
        1 gagcggccgg cgacgctgcg gcgctgacgc tatcggtcct cgagcacgtc cgcacccttg
       61 ggcgggacga actggaacag cccggcgtcg agcttcgggt tgcgctcgaa gctcttgaac
      121 gccagcaccg tggtctggcc gaaggtgtcg gcgatctgca tcgcgccggg cacgccgccc
      181 ttgaatccga tgcggatcgc gtcgaactgc gaatcgctcg tgcgcggctt ggcgacgatc
      241 cacgccagcc catcgcgctc gccgtcgtcg cgcacgtcga actcgcgctc gaagtcgttg
      301 gtgccgaaca ggatcgacgc ggggctggcc ggcaaggcgg cgccgatctt cttgaccgtg
      361 acctggttca ggtccttgtc gtagacgaag agccgctgcc cgtcggacac gatgacctgt
      421 tcgtacggct tgtcgtagct ccagcggaag cggcccggcc gctcgaattc gaagcgcccg
      481 gtcgacgaca ccggcggttt cttccccgcc gacacccgct ggctgaactc gccgcgcgcg
      541 ctcttcgtgt cggacagaaa gctgcgcaac tagtccaccg cgctcgcgtg cgcgatgttc
      601 accagcgcgg tcaatgcaac ggctgcaagc ggacgcaact aaggctcccg gtgcggcacc
      661 aggatctcgc ggttgccgtt gggctgcatc gcggacacca tgccggcctt ttccatgtct
      721 tcgagcaggc gggcggcgcg gttgtagccg atcctgaggt gccgctgcac gagcgagatc
      781 gacgcgcgcc ggttcttcaa cacgatttcc accgcctggt catataacgg atcagcctcg
      841 ccgccgggtt gaccttcgcc ttccatgccg ggcagcggcg cctccggcgc gtccagaatg
      901 ccctcgacat actgcggggc gccgcgcgcc ttcagcatct cgaccacccg gtgcacctcg
      961 gcgtccgaca cgaacgcgcc atgcacgcgc gtcggcagtc cggttcctcc tgccaggtac
     1021 agcatgtcgc cctggccgag cagcgtctcc gcgcccatct ggtcgaggat cgtgcgggag
     1081 tcgacgcggc tcgcgacctg gtaggcgatg cgcgacgcga tgttggcctt gatcaacccc
     1141 gtgatcacgt cgacgctcgg ccgctgggtg gcgaggatga gatgaatgcc cgccgcgcgc
     1201 gccttctgtg caaggcgaat gatcggctcc tcgaccttct tgccgatcac cagcatcagg
     1261 tccgccagct cgtcgatcac cagcacgatc atcggcagcg tgtcgagcgg ctccggcgct
     1321 tcgggcgtca gcgtgaacgg gttggggatc gtctcgccct ttgccttcgc ctcggcgatc
     1381 ttctggttgt aggtcgtcag cccgcgcacg cccaccttgc tcatcacgcg gtagcggcgc
     1441 tccatctccg ccacgcacca gttcagcgcg tgcgcggcct ccttcatgtc ggtgacgacg
     1501 ggcgccagca ggtgcgggat gccctcgtac accgagagct ccagcatctt cggatcgacc
     1561 agcagcagcc gcacctgcga cgggtcggcc ttgtacagga ccgacaggat catcgcgttg
     1621 atgccgaccg acttgcccga tccggtcgtg cccgcgacca gcaggtgcgg catccgtgcg
     1681 aggtcgatca ccaccggctt gccgccgatg tccttgccga gccccagcgt gaggagcgaa
     1741 tggctgtcgt tgtaggtctg cgagccgagg atctcggaca gcttgacgat ctgccgcttc
     1801 gggttcggca gctcgagccc catcaggttc ttgccgggaa tcgtctcgac cacgcggatc
     1861 gacaccagcg acagcgcgcg tgcgaggtcc ttcgcgagat tgacgatctg gctgcccttc
     1921 acgccgaccg cgggctcgat ctcgtagcgt gtgatcaccg gtcccggata cgcggcgacg
     1981 accgcgacct cgacgccgaa atccttcagc ttcttctcga tcagccgcga cgtgaactcc
     2041 agcgtctcgg tcgagatcgt ctcgacgttc gccggcggct cgtcgagcag atcgagcgcg
     2101 ggcagcgagc cgtccgccag ttccgcgaaa agcggcacct gcttttcctt ctgcttgcgt
     2161 tccgacggct tcacgcgcgc ctcggcgcgc tcgatgtgca cggggggcgc ctcgtcgatc
     2221 cgctcgcgct cctgttcgat catctgctcg cggctccgcg cgaccgcctc gccgatcgca
     2281 cggtcctcct ttgcttcgcg cgcggcgcgc gtgcgctgcg cgatcgactc gaaccagccg
     2341 ccgacgcgct cggcaagcgc gagccacgaa aaatcgaaga acaggctcgc gccgagggcg
     2401 atcgcgacga tgagcagcac ggaggtgccg gtgaatccga gcgcaccctt cagcgcgccc
     2461 gcgagcaggc ctccggacac cccgccgggt gcggctggca actgcacgcc gagcgtgtac
     2521 aggcgcagcg cctcgagcgt gctgcaggat gcgagcagca ccacgaaccc cacgccctgc
     2581 agccagtccg gcaggccgtg atcgcggtac tgtgcgtgca ggcgccggaa gccgcgcagc
     2641 gcccaagcgg caccgccgac cacccatagc cacgcggaaa agccgaacac cagcagcagc
     2701 acgtcggcga cccatgcgcc gacccgtccg ccgacgttgt ggatcagcgc gccggaggtc
     2761 gaatgcgaga acccgggatc ggcgcgactg taggtgatca ggatcgacgc cagcgcgatc
     2821 accgccgcgg cgccgacgat ccagcgcgct tcggcgagca ggcgggcgcc gcgcgatggc
     2881 ggctcgggcg ccgcggcgct gcgccgccgc gagtggacgc cgcccggctg aatcgttgat
     2941 tccatgcgac tcgacatcgg cgcagattat atggagacct tcgacgcgag atcgcgcaca
     3001 ccaccctttc gtctgcatta gcgcgagttc gtcgccgcgc cgggccggtt cgtcgcaccc
     3061 ttgccgccgg cgtgggcatg gcgggaaaat cgctccgctt gccgactccg ccatggcgac
     3121 cgccacctct gccgaccccg accgcgccgg attcgaatgg cgcgccgagc gcttccggca
     3181 cgaattcttc cggttcttca atttcggcac cgtggcggcg gtgttcttca tttgcctggc
     3241 ggtcgctttc acgcgctggc tgccgaccct gtacttcgac gcgccggcga tctgggcact
     3301 ggacggcatc cgctatctga ggcagaccct gatcagcggc ttgtgcgcgc tggccgcgat
     3361 cgcgctggtg caggcgctgg cgcgcgcgtt cgactggtcg cagcggcgca cgtacgcggt
     3421 gggcgtgatc gcgatcg
//
LOCUS       DBNHBY010000142         8807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_481452, whole genome shotgun sequence.
ACCESSION   DBNHBY010000142 DBNHBY010000000
VERSION     DBNHBY010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_481452"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC16HB_03375"
     CDS             complement(<1..182)
                     /locus_tag="AC16HB_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5605860.1"
                     /translation="MKWVTRERPKIDRIACPWLIARFIDREAEFLFVPSSQVQEVARS
                     ASAIPFDVPGVELTHVG"
     gene            complement(179..1105)
                     /locus_tag="AC16HB_03380"
     CDS             complement(179..1105)
                     /locus_tag="AC16HB_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HIR5605861.1"
                     /translation="MDFEALPLPFDAARLNGLSQRLIESHHRNNYDGAVRRLNAIRAR
                     SRALDLAQAPGFELNGLKREELLATNSLQLHEVYFDNLGGTGELEAGPLRTALAAAFG
                     SYERWRNEFTAMGRALAGGSGWVLLSWLPRGATLVNQWAADHAHACADATPLVALDMY
                     EHAYHLDYGAAAADYVQAFMRNLDWQRIAERFERAVAASAASIEILPESLRDHLSDWQ
                     VIDVRRRPVFDGAEHRIENARWFDPEQVDGWARDLDRARPVALYCVHGHAISRSTALA
                     LKRRGYDAYCVAGGIEAWQARALPLQAKGGGR"
     gene            complement(1119..2477)
                     /gene="chrA"
                     /locus_tag="AC16HB_03385"
     CDS             complement(1119..2477)
                     /gene="chrA"
                     /locus_tag="AC16HB_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR5605862.1"
                     /translation="MQVTSSSNEVAARPATVEFAVALRFWLKLGFISFGGPAGQIAIM
                     HQELVERRRWISESRFLHALNYCMLLPGPEAQQLATYIGWLMHRTWGGFVAGTLFVLP
                     SLALLIVLSWIFIAFGDVPAVAGLFYGIKPAVTAIVLHAAWRIGSRALKNRWLWAIAA
                     AAFIAIFVFDAPFPLIVAVAAACGVIGGRVMPDRFALGGGHADPKAASDSVAIIDDRM
                     PIPAHARYSTARLARTALVGIALWLAAMAALITGFGWQSTLTQMGWFFTKAALLTFGG
                     AYAVLPYVYQGAVEHHQWLSPAQMIAGLALGETTPGPLIMVVAFVGFVGAWQAQVFGP
                     DALFAAGAAAAGVVTFFTFLPSFVFIFAGAPFIETTHGDLRFTAPLTAITAAVVGVIV
                     NLALFFAWHVLWPHGRSGAFDWAAALIAIAAAIALFRFKAGVIPVIGACALAGLVMTL
                     FP"
     gene            complement(2477..3433)
                     /locus_tag="AC16HB_03390"
     CDS             complement(2477..3433)
                     /locus_tag="AC16HB_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5605863.1"
                     /translation="MDQKWIVLIATQPTGNAAVRMRLWREVRSRGAAILRDGVYLLPA
                     RPELAAAFTALAGAVEAAEGSAHVLNVHADDAQDADWRRLFDRGAEYADLMTQIQQRS
                     ARAKQPVEAARAACRELEEQFAAIATTDYFAAAAREQVAQALADWRARIERRATPDEP
                     QAQTRSIERLQREHFQGRVWATRRGLWVDRVASAWLIRRFIDPAARFAWIAKPDAKPK
                     RAIGFDFDGAQFTHQSGRVTFEVLLASFGLDEDAALVRLGCVIRALDVGGLPIAEAAG
                     IETVLAGLRRRHDDDDAFLATAGQVFDACHAGFARSESKEGG"
     gene            3572..4675
                     /gene="moaA"
                     /locus_tag="AC16HB_03395"
     CDS             3572..4675
                     /gene="moaA"
                     /locus_tag="AC16HB_03395"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5605864.1"
                     /translation="MTDRIIPLLDERVRAPRPVVPPVLETPTGRLNDRLARRLHDLRI
                     SVTDRCNFRCTYCMPREVFDVNYHFLPHAELLTFEEITRLARVFIDHGVEKIRLTGGE
                     PLLRKDIERLIEMLSALTTRAGQPLDLTLTTNASILARKARALAAAGLKRVTVSLDAL
                     DDAVFRRMNDADFPVAEVLRGIDAAAAAGLAPIKLNMVVKRGTNDREIVPMARHFRAS
                     GHILRFIEFMDVGTSNGWRMDDVVPSAEVIERIGAEWPVEPIDPNYEGEVAERWRYRD
                     GAGEIGVISSVTQAFCRNCTRARLSTEGKLYLCLFAQSGHDLRALVRGGASDAQISTA
                     IAHIWRARDDRYSELRSEATARLPKIEMSYIGG"
     gene            complement(4681..6099)
                     /locus_tag="AC16HB_03400"
     CDS             complement(4681..6099)
                     /locus_tag="AC16HB_03400"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006081 - aldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HIR5605865.1"
                     /translation="MMPSEFPLQMQALFETQRAAFAREPYPTWPTRRDRLQRLRKLLT
                     ENERALEAAIDHDFGHRPGIETQLAEIFPSLEEIKAALRDGREWMKARHAPVSKWFLP
                     ARGKLIPKPLGVIGIIVPWNYPVFLAVGPMVGALAAGNRVMLKLSEHTPAFSACFTQL
                     VARYFAADEVAVVLGGADVAAEFSGLPFDHLLFTGSTLVGKTVMAAAAKNLTPVTLEL
                     GGKSPTVIAPGYPLRKAATRILAGKLLNAGQTCIAPDYVLLPRGQVDAFVAEARTKAT
                     RMYPDGVASDDYCSIINERHYRRLSGYLEQATAAGARIEQMFAGATRDDTARKLAPTL
                     IVDPPLQLAAMQEEIFGPLLPLVPYDRIEDAVEFVTRQPHPLALYWFDRNEKRIEWAL
                     QNTRAGGISINDTLLHIAQDALPFGGIGPSGMGHYHGRWGFDTFSKLKPVFRQAHVNA
                     MGIFLPPYRPLARRLLEVMKRF"
     gene            complement(6370..7956)
                     /gene="aceB"
                     /locus_tag="AC16HB_03405"
     CDS             complement(6370..7956)
                     /gene="aceB"
                     /locus_tag="AC16HB_03405"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR5605866.1"
                     /translation="MTLPQGVEIKAPLKAGYDRILSADALALVTKLHRAFEPRRRELL
                     ARRAERARRLDAGEHPDFLADTKSIRDGNWKIAPIPADLQCRRVEITGPVERKMVINA
                     LNSGADVYMTDFEDSNTPNWDNQIQGQLNLIDAIRRTITLEQGGKVYRLNDKVATLLV
                     RPRGWHLDEKHVQVDGQRISGGIFDFALFMFHNAKELLARGTGPYFYLPKLESHLEAR
                     LWNDVFVMTQKELGIPNGTVKATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIF
                     SCIKKFKVDRNFCLADRARVTMTAPFMRAYALLLLKTCHKRGAHAMGGMSALIPIKND
                     PVANEKALGGVRSDKARDATDGYDGGWVAHPGLVGLAMEEFVKVLGDRRNQIDKQRDD
                     VQVAAADLLNFQPEQPITETGLRLNINVGIHYLGSWLAGNGCVPIHNLMEDAATAEIS
                     RSQVWQWIRSPKGKLEDGRKVTVELVRSLIPGELAKVKADVGAEAGPTYDRAAQIFEQ
                     MSTQETFAEFLTLPLYEEIQ"
     gene            complement(7965..8648)
                     /locus_tag="AC16HB_03410"
     CDS             complement(7965..8648)
                     /locus_tag="AC16HB_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR5605867.1"
                     /translation="MTIRAVLFDVFGTLLDVQSVWARADQLFPGNGASLARLWREKQL
                     EYTRLRTISSRYVSFTRVTEDALQFACDALGLPLDAAARGMLMHEYTQLDPFSDAIPA
                     LKKLEQAGVTMGVLSNGDPGLLDDVLHGARLADYFDVVLSADQVHAFKTAPAVYELGP
                     LTLKHPAGEIVLVSSNAWDAIGAKWYGYVSFWVNRGGGPLDRLGERPDAIGTSLDDAA
                     SFVLQSVKK"
BASE COUNT         1531 a         2991 c         2802 g         1483 t
ORIGIN      
        1 ccaacgtggg tcagttcgac ccccggcacg tcgaagggga tcgccgaagc gctgcgtgca
       61 acctcctgca cctgcgagct cggaacgaac aggaattcgg cttcacggtc tatgaagcgc
      121 gcgatcagcc acgggcaggc aatgcgatcg atcttcggcc gctcccgcgt cacccatttc
      181 atcgtccacc tcctttggct tgcagcggca gtgcacgcgc ctgccacgcc tcgattccac
      241 cagccacgca gtacgcgtcg tagccgcgcc gcttcaacgc gagtgccgtg ctgcggctga
      301 tcgcgtggcc gtgcacgcag tacagcgcca caggccgcgc tcgatcgaga tcgcgcgccc
      361 agccgtcgac ctgctccgga tcgaaccagc gcgcgttctc gatccgatgc tcggcgccgt
      421 cgaacaccgg ccggcgccgc acgtcgatca cctgccagtc ggacagatgg tcccgcagcg
      481 actcgggcag gatctcgatc gacgccgcgc ttgccgccac cgcgcgttcg aagcgttcgg
      541 cgatccgctg ccagtcgaga ttccgcatga acgcctgcac gtagtccgcc gcggcagcgc
      601 cgtagtcgag gtggtatgcg tgctcgtaca tgtcgagcgc gacgagcggc gtggcgtccg
      661 cgcatgcgtg cgcgtggtcg gcggcccact gattgaccag cgttgcacca cgcggcagcc
      721 acgacagcag cacccagccc gatccgcccg cgagcgcccg gcccattgcg gtgaactcgt
      781 tgcgccagcg ttcgtacgaa ccgaaagccg ccgccagtgc ggtgcgcagt ggtccggctt
      841 cgagttcgcc cgtcccgccg aggttgtcga agtacacctc gtgcagctgc agcgaattgg
      901 ttgcgagcag ctcctcgcgt ttcagtccgt tcagctcgaa gccgggtgcc tgcgcgagat
      961 cgagcgcacg tgacctcgcg cggatcgcgt tcaggcgtcg cacggcgccg tcgtagttgt
     1021 tgcggtgatg gctttcgatc aaccgctgcg acaggccgtt caaccgggcc gcatcgaacg
     1081 gcaggggtag cgcctcgaag tccatgtcgt tctccgaatc acggaaagag tgtcatcacg
     1141 agccccgcca gcgcgcaggc gccaatgacg ggtatcaccc cggccttgaa gcgaaagagt
     1201 gcaatcgctg ccgcgatcgc gatcagtgcg gcggcccagt cgaacgcgcc gcttcgaccg
     1261 tgcggccaca gcacgtgcca ggcgaaaaag agcgccagat tgacgatcac cccgaccacg
     1321 gccgcggtga tcgcggtcag cggcgcggtg aagcgcagat cgccgtgcgt ggtctcgatg
     1381 aagggcgcgc cggcgaagat aaagacgaac gacggcagaa aggtgaagaa cgtcaccacg
     1441 cccgccgctg ccgcacccgc ggcgaagagc gcgtcgggcc cgaacacctg tgcctgccac
     1501 gcgccgacaa agccgacgaa cgccaccacc atgatcagcg gtccaggcgt cgtctcgccg
     1561 agcgcgagcc ccgcaatcat ctgtgccggc gacagccact gatgatgctc gaccgcgccc
     1621 tgatagacgt agggcagcac cgcataagcg ccgccaaagg tcagcagcgc tgccttggtg
     1681 aagaaccaac ccatttgagt cagcgtcgac tgccagccaa agcccgtgat cagcgcagcc
     1741 atcgccgcca gccacagggc gatgccgacg agcgcggtgc gcgccagtcg cgccgtcgag
     1801 tagcgcgcat gcgcagggat cggcatccgg tcgtcgatga tcgccacgga atcggatgcc
     1861 gctttgggat ctgcatgccc gccgccaagg gcgaagcggt ccggcatgac ccgcccgccg
     1921 atcacaccgc aggcagcagc gaccgcaacg atcagcggga acggggcatc gaaaacgaag
     1981 atcgcgataa acgccgcagc ggcgatcgcc catagccacc ggttcttcag cgcacgcgat
     2041 ccgatccgcc aagccgcgtg cagcacgatc gcggtgaccg ccggcttgat gccgtagaac
     2101 aatccggcca ccgccggtac gtccccgaat gcgatgaaga tccacgacaa cacgatcagc
     2161 agcgcgagcg acggcagcac gaacagcgtg ccggcaacga agccgcccca ggtccggtgc
     2221 atcagccagc cgatgtaggt cgcaagttgc tgcgcctcgg gccccggcag cagcatgcag
     2281 tagttcagcg cgtgcaggaa gcgactctcg gagatccacc gacggcgctc gaccagttcc
     2341 tggtgcatga tcgcaatctg ccccgcgggc ccgccgaagc tgatgaaccc gagcttcagc
     2401 cagaagcgca gcgcgacggc gaattcaacg gtggccggcc ttgccgcgac ctcatttgac
     2461 gaggaagtca cctgcatcag ccaccctcct tcgattcgga tcgcgcaaat cctgcgtgac
     2521 acgcatcgaa cacctgtccg gcggtggcga gaaacgcgtc gtcgtcgtcg tgccgtcgcc
     2581 gcaggccggc gagcaccgtt tcgattcccg ccgcttccgc gatcggcagg ccaccgacgt
     2641 cgagcgcgcg aatcacacag ccgagccgca ccaacgcagc gtcttcgtcc agcccgaaac
     2701 tcgccagcag cacctcgaac gtcacgcgac ccgactggtg cgtgaactgc gcaccgtcga
     2761 aatcgaaccc gatcgcccgc tttggcttgg catccggctt tgcgatccat gcgaagcgcg
     2821 ccgccggatc gatgaagcgg cggatcagcc atgcgctggc gacgcggtcg acccacaagc
     2881 cgcggcgcgt cgcccatacg cggccctgga aatgctcccg ctgcaaccgc tcgatcgagc
     2941 gtgtctgcgc ctgcggttca tccggcgttg cgcgtcgctc gatccgcgcg cgccagtccg
     3001 ccagcgcctg tgcgacctgc tcgcgtgccg cagcggcgaa gtagtcggtc gtggcaatcg
     3061 ccgcaaactg ttcctccagt tcgcggcacg ccgcgcgcgc agcttcgacc ggctgcttgg
     3121 cgcgcgcgga gcgctgctgg atctgggtca tcagatcggc gtactcggca ccacgatcga
     3181 acaaccggcg ccaatcggca tcctgtgcat cgtcggcgtg cacgttcagc acgtgtgccg
     3241 aaccttctgc tgcctctacg gcgcccgcca gcgccgtgaa cgccgcggca agctccggcc
     3301 gcgccggcag cagatagacc ccgtcgcgga ggatcgccgc gccgcggctg cgcacctcgc
     3361 gccacagacg catgcgtacc gccgcattgc cggtgggctg cgtagcgatc aatacgatcc
     3421 acttctggtc catcgacaga tgccatcgaa atgttgttgc atttataact tctatgtagt
     3481 atctataaca tcccataatc acctgatgca ggcagggcga cttcttcagg ccgcaggttg
     3541 cgacgccgcc ggcggccggt aacatcgccg catgactgat cggatcattc ctctgctcga
     3601 cgagcgcgta cgcgcgccgc gtcccgtggt tccgcccgtt ctcgaaacgc cgacgggccg
     3661 gctgaacgac cggctggcgc gccgactgca cgatctgcgc atctcggtca ccgaccgctg
     3721 caacttccgc tgcacgtact gcatgccgcg cgaagtgttc gatgtcaact atcactttct
     3781 gccacacgcg gaactgctga ccttcgagga aatcacgcgg ctcgcgcgcg tcttcatcga
     3841 tcacggcgtc gaaaagatcc gcctcaccgg tggcgagccg ctgctgcgca aggacatcga
     3901 gcgcctgatc gagatgcttt ccgccttgac gacgcgcgcc ggccagccgc tcgacctgac
     3961 cctgacgacc aatgcgtcga tcctggcgcg caaggcgcgg gcgctcgcgg cggcagggct
     4021 gaaacgcgtg acggtgtcgc tcgacgcgct cgacgacgcg gtgttccggc gcatgaacga
     4081 cgccgacttc ccggtcgccg aggtgctgcg cggcatcgac gctgcagccg cagcgggtct
     4141 ggcgccgatc aagctcaaca tggtcgtcaa gcgcggcacc aacgaccgcg agatcgtgcc
     4201 gatggcgcgc cactttcgcg cgagcggcca catcttgcgc ttcatcgaat tcatggacgt
     4261 cggcacgtcg aacggctggc ggatggacga cgtggtgccg tcggccgagg tgatcgagcg
     4321 catcggagcc gaatggccgg tcgaaccgat cgacccgaac tacgaaggtg aagtcgctga
     4381 gcgctggcgt taccgcgacg gcgcgggaga aatcggcgtg atctcgagcg tcacgcaggc
     4441 attctgccgc aactgcacgc gcgcacggct atcgaccgaa gggaaactgt atctctgcct
     4501 gttcgcgcag agcgggcacg atctgcgcgc gctggtgcgc ggcggcgcca gcgacgccca
     4561 gatctcgacc gcgatcgcgc acatctggcg cgcgcgtgac gaccgctact cggagctgcg
     4621 cagcgaggca accgcgcgcc tgccgaagat cgagatgtcg tacatcggcg gctgagtcgc
     4681 ctagaaccgc ttcatcactt cgagcaggcg ccgcgccagc ggccggtacg ggggcaggaa
     4741 gattcccatc gcgttgacgt gcgcctggcg gaacacgggt ttgagcttgc tgaacgtgtc
     4801 gaagccccag cgcccgtgat agtgacccat gcccgacggt ccgatgccgc cgaatggcag
     4861 tgcgtcctgc gcgatgtgca gcagggtgtc gttgatactg atgccgccgg cgcgcgtgtt
     4921 ctggagcgcc cattcgatgc gcttctcgtt gcggtcgaac cagtacagcg ccaacgggtg
     4981 cggctgccgc gtgacgaact cgaccgcgtc ctcgatccgg tcgtacggaa cgagcggcag
     5041 caagggaccg aagatctctt cctgcattgc ggccagttgg agcggcggat cgacgatcag
     5101 cgtcggggcc agcttgcgcg ccgtgtcgtc gcgcgtcgcg ccggcgaaca tctgctcgat
     5161 gcgcgcgccg gccgcggttg cctgttccag atagccgctc agccgccggt agtggcgctc
     5221 gttgatgatg ctgcagtagt cgtcgctcgc gacgccgtcg ggatacatgc gcgtcgcctt
     5281 cgtgcgcgcc tcggccacga acgcatcgac ctgcccgcgc ggcagcagca cgtagtcggg
     5341 cgcgatgcag gtctgccccg cgttcagcag cttgccggca aggatgcgcg tcgccgcctt
     5401 gcgcagcgga tagccgggcg cgattaccgt cggcgacttg ccgccgagct cgagcgtgac
     5461 cggcgtgagg ttcttcgcgg cagccgccat cacggtcttg ccgaccagcg tcgagccggt
     5521 gaacagcaga tgatcgaacg gcaggccgct gaactccgcc gcgacgtcgg caccgccgag
     5581 cacgaccgcc acttcatcag cggcgaagta gcgcgcgacg agctgcgtga agcatgcgga
     5641 gaacgccggc gtgtgctcgg acagcttcag catcacgcgg ttacccgcgg cgagcgcacc
     5701 caccatgggc ccgactgcga ggaacaccgg gtagttccac ggcacgatga tgccgattac
     5761 gccgagcggc ttcgggatca gcttgccacg tgcgggcaga aaccacttgg ataccggcgc
     5821 gtggcgcgcc ttcatccatt cgcggccgtc gcgcagcgcg gccttgatct cctccagact
     5881 gggaaagatc tccgcgagct gagtttcgat ccccgggcgg tgtccgaagt cgtgatcgat
     5941 cgcagcttcc agcgctcgtt cgttctccgt cagcagcttg cgcagccgct gcagtcggtc
     6001 acggcgcgtc ggccaggtcg ggtacggctc gcgcgcgaag gcggcgcgct gcgtctcgaa
     6061 cagagcctgc atctgtagcg gaaactcgga cggcatcatg gcgagtcgct cggtagtcaa
     6121 ctcactcaat gctagtcgcg aaagacgaac ggccgctgga gcggccgttc taaagcgcaa
     6181 gtctcggcta gcgcttcctt gccttcgcct ttggcggggg tgattccgca cgctcaccgg
     6241 gctccacctt cttctggtcg ccgatcacgg tcttcaatgg acagaccgag aaggccgggc
     6301 atttgcggca gccggcaacg accgcaatcg ggcacagtgt catgttgggg tcctccttat
     6361 cggcgcggcc tactggatct cttcgtaaag cggcagcgtc agaaactcgg cgaaggtctc
     6421 ctgcgtgctc atctgctcga agatctgcgc ggcacgatcg taggtcggac cggcttcggc
     6481 gccaacgtcg gccttgacct tggcgagttc cccgggaatc agggatcgca ccagttcgac
     6541 cgtcaccttg cggccgtcct ccagcttgcc cttgggcgag cggatccatt gccacacctg
     6601 cgaacggctg atctccgcgg tggcggcgtc ttccatcagg ttgtggatcg gcacacagcc
     6661 gttgcccgcg agccacgagc cgaggtaatg gatgccgaca ttgatgttca ggcgcagtcc
     6721 cgtctcggtg atcggctgct cgggctggaa attcagcaga tcggcggccg cgacctgcac
     6781 gtcgtcacgc tgcttgtcga tctggttcct gcgatcgcca agcaccttga cgaattcctc
     6841 catggccagc ccgaccagtc ccgggtgcgc gacccagccg ccgtcgtagc cgtcggtcgc
     6901 gtcgcgcgcc ttgtcgctgc gcacgccgcc gagcgccttc tcgttcgcga ccggatcgtt
     6961 cttgatcgga atcagtgcgc tcatgccgcc catcgcgtgc gcgccacgct tgtggcaggt
     7021 ctttagcagc agcagcgcgt atgcacgcat gaacggcgcg gtcatcgtca cgcgcgcgcg
     7081 atcggcgagg cagaaattcc ggtcgacctt gaacttcttg atacacgaga agatatagtc
     7141 ccagcggccc gcattgagtc cggcgctgtg ctcgcgcagc tcatagagga tctcgtccat
     7201 ttcgaacgcg gcaaggatcg tctcgatcag gacggtcgct ttgacggtgc cgttcggaat
     7261 gccgagctcc ttctgcgtca tcacgaacac gtcgttccag agccgtgcct caaggtggga
     7321 ctccagcttc ggcaggtaga agtaaggacc cgtgccacgg gccagcaatt ctttcgcgtt
     7381 gtggaacatg aagagcgcga agtcgaagat gcctcctgaa atgcgctgcc cgtcaacctg
     7441 cacgtgcttt tcgtcgaggt gccagccgcg cgggcgcacc agcagggtcg cgaccttgtc
     7501 attcagtctg tagaccttgc cgccctgctc cagcgtgatc gtgcggcgga tggcgtcgat
     7561 caggttgagc tggccctgga tctggttgtc ccagttcggc gtgttcgaat cctcgaagtc
     7621 ggtcatgtag acgtcggcgc ccgagttcag ggcgttgatg accatcttgc gctcgaccgg
     7681 accggtgatc tccacgcggc ggcactgcaa gtcggccggg atcggagcga tcttccagtt
     7741 gccgtcacga atcgacttgg tatccgcgag gaaatccggg tgctcgccgg catcgagccg
     7801 gcgcgcgcgc tcggcccgcc gggcaagcag ttcgcggcgg cgcggttcga atgcgcggtg
     7861 cagcttggtg accagcgcaa gagcgtcggc cgagaggatc cggtcatagc cggctttcag
     7921 gggcgccttg atttcgacgc cttgcggcag agtcatcact tctcctactt cttcacggat
     7981 tgcagaacga aactggcggc atcatccagt gacgtgccga tggcgtcggg gcgctcgccg
     8041 agacgatcga gcgggccgcc gccacgattg acccagaagc tcacgtagcc gtaccacttc
     8101 gcgccgatgg catcccacgc gttgctgctg acgagcacga tctcgcccgc cggatgcttc
     8161 agcgtcagcg gaccgagctc gtacaccgct ggcgcggtct tgaacgcatg tacctggtcg
     8221 gccgacagca cgacgtcgaa atagtcggcc aggcgcgcgc catgcagcac gtcgtcgagc
     8281 aggccaggat cgccattgct gagcacgccc atcgtcaccc cggcctgctc gagcttcttc
     8341 agcgccggaa tcgcatcgga aaacggatcg agctgcgtgt actcgtgcat cagcattccg
     8401 cgcgcagccg cgtcgagcgg caagcccaat gcatcgcagg cgaactgcag cgcgtcttcc
     8461 gtcacgcgcg tgaacgacac gtagcggctc gaaatcgtgc gcagccgcgt gtattccagc
     8521 tgcttctcgc gccacagtcg cgccagcgat gcaccgttgc cgggaaacag ctgatcggcg
     8581 cgcgcccata ccgactgcac gtcgagcagg gtcccgaaca catcgaaaag cactgcacga
     8641 atcgtcatga tttcccctcc aatgcgctca gtatattggg gtcgcgcatc gagcgtcggc
     8701 gcgcctcgga gcgaaaaaaa gggccgcttg cgcggcccga ttcgtttgta ttgatctcga
     8761 tcttcttgac tgcgtctcct tcgacctcgc ccttctcctt cggtacg
//
LOCUS       DBNHBY010000143         6599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1699853, whole genome shotgun sequence.
ACCESSION   DBNHBY010000143 DBNHBY010000000
VERSION     DBNHBY010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1699853"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="AC16HB_03415"
     CDS             complement(<1..280)
                     /locus_tag="AC16HB_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605868.1"
                     /translation="MTVRPPTAPVAHWKRFRTLYLLAALCTAPVIASYTAYYLLPPTG
                     RTNYGDLVEPQRPMPALALRELDGRDVATSALRGRWLLVTVDSGDCRDA"
     gene            complement(294..968)
                     /locus_tag="AC16HB_03420"
     CDS             complement(294..968)
                     /locus_tag="AC16HB_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR5605869.1"
                     /translation="MLGIALTVSLGNWQTRRAQYKVELQAHWDAAGLAPPIVVNAATI
                     ADIETRTPVRVRLTGRFDHRHSVWLDNRPSHGRAGFHVITPLVTSGGIVLVVRGWAAR
                     DPQDRLLLPAVGEPAGELTIEGLALKDWPRLLELGSPGESERWPRVWQNLDYAAYERA
                     SGLNVAPFVVEQTSRTDDELVRDWPAPDLGIDMHRGYALQWYSLAALIAGLTLFFGWR
                     AWRAAQ"
     gene            1062..1259
                     /locus_tag="AC16HB_03425"
     CDS             1062..1259
                     /locus_tag="AC16HB_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR5605870.1"
                     /translation="MKAFIAVAFVAVLGALGSALVFLMKDKGQGTRTLNALRLRVGLS
                     IALLLLIWFSWYMGWISPRTY"
     gene            1684..3636
                     /locus_tag="AC16HB_03430"
     CDS             1684..3636
                     /locus_tag="AC16HB_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR5605871.1"
                     /translation="MPARTELFSHFQEHFARAQDEEMSLGDFLHFCKDDRMAYASAHE
                     CVLEAIGKPELLNTAEDPRLSRIFQNRVIQTWDTFSEFHGMEETLMNLYAYFLHAAQG
                     LEERKQVLYLLGPVGGGKSSLAEKIKSLCEQFPIYALKDSLTGKVSPLLESPLGLFNR
                     DRYGAQMEDKFDIPRRALTGIASPWALKRLKEYNGDLNKFRVVRVMPSVLNQIGIVKT
                     EPGDENTQDISSLVGKVDLRMLEQYAQDDPDAYSYSGALCLGSARCVEFVEMFKAPLK
                     VLNPILTATQEGNFKGTEALPAMPFNGFIMAHSNEAEWLKFRNNKDNEAFIDRVYLVK
                     VPYCMRVDEEVRIYDKMLKASTLSEAPCAPQTLRTLAEFTVLSRLTVPENSSVYSKLR
                     AYNGENLKDIDPRSKSVKEYRDDAGLAEGFSGLSTRWAFKTISSAFNRDPSEIAADPV
                     NLLVVLEDRLNKDDLPEEYKRRLRSHIKEWIVPRFAEFLEKELQMSYLDAYGEYGQNL
                     FDRYVTYADKWLLDEDWRDPDTGMQYSRDALNAELEKIEKPAGISNPKDFRNEVVNFV
                     LRARAHGKNPHWTSYEKLRVVIEKRMFSKTEDILPVISFEAKGSQELDDRHKSFVNRM
                     VARGYTEKQVRRLVDWYLHYKKSAQS"
     gene            3692..4963
                     /locus_tag="AC16HB_03435"
     CDS             3692..4963
                     /locus_tag="AC16HB_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HIR5605872.1"
                     /translation="MMRIVDRRGDPPGRGAVNRERFVRRFKDDIKRAADDEFAKRSIK
                     DIGRGGKISIPGRGLKEPFLHHDDKTGNRDFVLPGNRDFLPGDAIPRPSGGGQGGPEG
                     DEPGEGEGRDNFAFVLSRDEFLSLFFDDLELPNLERTRFGEVTMERTRRAGFSRDGAP
                     SNLLPAMTVRMSIARRIALRGAAGERIDDLIDELAGAPHDEKDEIRAEIARLLERRDR
                     LPFLEDVDRRYRARAPMPAPATRAAMICLMDVSGSMDERKKDLAKRFFALLYMFLERK
                     YGAVEIVFIRHTETAQEVDEKTFFYDPQSGGTLVMPALELARKVIDERFTGSDWNVYI
                     AQASDGDCGEDDATRCAGYVKSDLLPRVRYFAYVDIPSNASGGWFSRPSDLWQCYEAV
                     SDDSFAKRKVHDRRDIWPVFRDLFARREAVA"
     gene            5047..6546
                     /locus_tag="AC16HB_03440"
     CDS             5047..6546
                     /locus_tag="AC16HB_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HIR5605873.1"
                     /translation="MPRAERPRREPIATGTEWTVPLLERFDTAIAGHAKRLGLVTFPI
                     QYEVITADQMLDLCSTVGMPVHYPHWSFGKQLVSQERGYRKGRAGLAYEIVINTNPSL
                     VYLMETNTLALQVLVMAHAAYGHNAFFRNNYLFQQFTQPDAILDYLKFARGYIVECEQ
                     RYDWRDVEKVLDCCHAIAPYGVDRYRRPGRLSAKRERERQVERLRFAERHFNPDYAHM
                     YAERKEEPSAPAFEPQENLLYFIEKSAPKLAPWQRELVRIVRKVSQYFYPQRLTKVAN
                     EGAATFWHYTLLHELYDAGQVDDGFMLEWLANHSDVVTQLPFDSQHYHGINPYALGFA
                     IMRDIRRICEQPTDEDRQWFPHLAGREWTEQIHFAMANFKDESLIEQYLSPKVMRDMR
                     LFMLADMEQSRDEYRVEAIHNEQGYLRVRQTLAAQYRPEHYLPQIVVARDSADSDRAL
                     LLQHRVEHGRLLEAEAVAEVLGYVKELWQFDVVLEEIDDAGNRLKIHRT"
BASE COUNT         1267 a         2097 c         2143 g         1092 t
ORIGIN      
        1 aggcgtcgcg gcagtcgccg ctgtccacgg tgaccagcag ccagcgaccg cgcagcgcag
       61 atgttgccac gtcgcgccca tcgagttcgc gcagcgcgag cgcaggcatc gggcgctgcg
      121 gctcgaccag atcgccgtag ttggtccgcc ccgtgggcgg cagcaggtag taggcggtgt
      181 acgacgcaat gaccggcgcc gtgcaaagcg cggcgagcag gtacaacgtg cggaatcgct
      241 tccagtgagc gaccggggcg gtcgggggac gcaccgtcat gtgcagaata gggtcattgc
      301 gccgcgcgcc aggcgcgcca gccgaagaac agggtcagcc ccgcgatcag cgccgcaagc
      361 gaataccact gaagcgcgta gccgcggtgc atgtcgattc caaggtcggg tgccggccag
      421 tcccgcacca gttcgtcgtc ggtccgcgaa gtctgctcga ccacgaaggg cgccacgttg
      481 agcccgctcg cgcgctcgta cgccgcatag tcgagattct gccaaacgcg cggccatcgc
      541 tccgattcgc cgggcgatcc cagttcaagc agccgcggcc aatccttgag tgccagccct
      601 tcgatcgtca attcgccggc aggttcgccc acggctggca acagcaggcg gtcctgcgga
      661 tcgcgcgctg cccatccgcg cacaaccagc acgatgccgc cgctcgtcac caagggcgtt
      721 atgacatgga agccggcgcg accatgactc gggcgattgt cgagccagac gctgtggcga
      781 tggtcgaagc ggccagtcag gcgcacccga accggggtgc gagtctcgat gtccgcgatc
      841 gtcgctgcgt tgacaacgat gggtggggca agcccagcag cgtcccagtg cgcctgcagt
      901 tcgaccttgt attgggcgcg gcgcgtttgc caattgccga gcgacaccgt cagggcgatg
      961 ccgagcacgc cgacgatcag cgcgacgatc gtgcggcggc ggttcatcgc gggaaccgga
     1021 agccccgatg ggataatgaa gtcgcgccca gcgcccgtcc catgaaagcc ttcatcgccg
     1081 tcgcattcgt cgccgtcctt ggcgccctcg gttctgcgtt ggtgttcctg atgaaggaca
     1141 agggccaggg cacccgtacg ctcaacgccc tgcgcctgcg cgtcgggctg tccatcgctc
     1201 tgctgctgtt aatctggttc agctggtaca tgggctggat cagcccgcgc acttactgac
     1261 cgcaagcggg cggccgatcg tcggggcgac cgtgtcgtcc tccatatcac cgccggggtc
     1321 tggacatttc attcgacata tggtgatcta atggacagcg cgcgcgtcca agaaaaaggg
     1381 gagaaacgac gacgagtgaa ctttcgcggt ggcagacgaa actaacatga gtgaatgttt
     1441 cgaaccgaat cggcgcccgg ctctcgccgg cagcgaatgt cgaatcctcg ccactccaat
     1501 gatccgcgcc gccattcccc tgctccgatc gggattccgt aaagattcgg ccgaccgcac
     1561 gatagccaac acagtggcgg gcgtttactc ttctagtagc cgcaccgcga caggcacttt
     1621 gtcaactagt ctggcttcat cgcgtttgct tcggtgccgg ccctcagggc caaggaggtc
     1681 atgatgccag cgcgaacgga actgttctcg cacttccagg aacacttcgc ccgggcccag
     1741 gacgaggaga tgagcctggg agacttcctg cacttctgca aagacgatcg catggcctat
     1801 gccagcgcac acgagtgcgt gctcgaggcg atcggcaagc cagaactcct gaacactgcc
     1861 gaggatccgc ggctgtcgcg catcttccag aaccgcgtga tccagacgtg ggacacgttc
     1921 tccgagttcc acggcatgga agaaacgctg atgaatctgt acgcgtactt cctgcacgcc
     1981 gcgcagggcc tggaagagcg aaaacaggtg ctctacctgc tcggcccggt cggtggcggc
     2041 aagtcgtcgc tggccgagaa gatcaagtcg ctgtgcgagc agttcccgat ctacgcattg
     2101 aaggattcct tgactggcaa ggtgtcgccg ctgctggagt cgccgttggg tctgttcaac
     2161 cgcgaccgct acggcgctca gatggaggac aagttcgaca tcccgcggcg agcgctgacc
     2221 ggcatcgcga gtccgtgggc actgaaacgg ctgaaggaat acaacggcga tctgaacaag
     2281 ttccgcgtcg tacgcgtcat gccgtcggtg ctgaaccaga tcggcatcgt gaagaccgag
     2341 cccggtgacg aaaacacgca ggacatctcg tcgctggtcg gcaaggtcga cctgcgcatg
     2401 ctcgagcagt acgcgcagga cgatccggac gcttacagct actcgggcgc actgtgtttg
     2461 gggtccgcgc gctgcgtgga gttcgtcgag atgttcaagg cgccgctgaa ggtgctcaat
     2521 ccgatcctga ccgccacgca ggaaggcaac ttcaagggca ccgaggcgct gccggcgatg
     2581 ccgttcaacg gcttcatcat ggcgcactcc aacgaggcgg aatggctgaa gttccgcaac
     2641 aacaaggaca acgaggcctt catcgaccgc gtgtatctcg tcaaggtgcc gtactgcatg
     2701 cgcgtcgacg aggaggtccg catctacgac aagatgctga aggcctccac gctatcggag
     2761 gcgccgtgcg cgccgcagac gctgcgcacg ctggccgagt tcaccgtgct ttcgcggctg
     2821 acggttccgg agaattcgtc cgtctattcg aagctgcgcg catacaacgg cgagaacctg
     2881 aaggacatcg atccgcgctc caagagcgtg aaggagtatc gcgacgacgc cgggctggcc
     2941 gaaggcttct cgggcctttc gacgcgctgg gcgttcaaga cgatctcatc ggcgttcaac
     3001 cgcgatccga gcgagatcgc ggccgacccg gtgaacctgc tggtggtgct ggaagatcga
     3061 ctgaacaagg acgacctgcc ggaggaatac aagcgtcgcc tgcgctcgca catcaaggag
     3121 tggatcgtcc cgcgcttcgc cgagttcctg gagaaggaac tgcagatgtc gtatctcgac
     3181 gcgtacggcg agtacggcca gaacctgttc gaccgctacg tgacatacgc ggacaagtgg
     3241 ttgctcgacg aggactggcg cgatccggac accggcatgc agtactcgcg cgacgcgcta
     3301 aatgcggagc tggaaaagat cgagaagccg gccggcatca gcaacccgaa ggacttccgc
     3361 aacgaggtcg tcaatttcgt gttgcgcgcg cgtgcccacg gcaaaaaccc gcactggacc
     3421 agctacgaga agctgcgcgt cgtgatcgag aagcggatgt tcagcaagac cgaggacatc
     3481 ctgccggtaa tctcgttcga ggccaagggc tcgcaggagc tcgacgatcg gcacaagagc
     3541 ttcgtcaacc gcatggtcgc gcgcggctac accgaaaagc aggtgcgacg cctcgttgac
     3601 tggtatctgc actacaagaa gtcggcgcag tcctgagcca ccgccgccga tatgtgaggc
     3661 cgcgatggcc gacctggtcg cacccggcaa actgatgcgg atcgtcgatc gccgtggcga
     3721 tccgccgggg cgcggcgcgg tgaaccgcga acgcttcgtg cgccgcttca aggacgacat
     3781 caagcgcgcc gcggacgacg agtttgccaa gcgatcgatc aaggacatcg gccgcggcgg
     3841 gaagatcagc atccccggcc gcggtctgaa ggagccgttc ctccatcacg acgacaagac
     3901 cggcaaccgc gatttcgtgc tgccaggcaa tcgcgacttt ctgccgggcg atgcgatccc
     3961 gcgtccgagc ggcggcggcc agggcgggcc ggagggcgac gagccaggcg aaggcgaagg
     4021 ccgcgacaac ttcgcgttcg tgctgtcgcg cgacgagttt ctgtcgctgt tcttcgacga
     4081 tctcgaactg ccgaacctcg agcgcacccg cttcggcgag gtcacgatgg agcgcacgcg
     4141 ccgggcgggc ttcagccgcg acggcgcgcc gagcaacctg ctgccggcga tgacggtgcg
     4201 catgtcgatc gcacgccgca tcgccctgcg cggcgccgcc ggcgagcgca tcgacgacct
     4261 gatcgacgag ttggcgggcg cgccgcacga cgaaaaggac gaaatccgtg ccgagatcgc
     4321 gcgccttctc gagaggcgtg atcggctgcc gttcctggag gacgtcgatc gccgctaccg
     4381 cgcgcgggcg ccgatgccgg cgccggccac gcgcgcggcg atgatctgcc tgatggacgt
     4441 gtcgggctcg atggacgagc gcaagaagga cctcgccaag cgcttctttg cgctcttgta
     4501 catgttcctg gagcgcaagt acggcgcggt cgagatcgtc ttcattcgtc acaccgagac
     4561 ggcgcaggaa gtcgacgaga agaccttctt ctatgacccg caatccggcg gcacgctggt
     4621 gatgcccgcg ctcgaactgg cacgcaaggt gatcgacgaa cgcttcacag gcagcgactg
     4681 gaacgtgtac atcgcgcagg cctccgacgg cgactgcggc gaagacgatg cgacgcgctg
     4741 cgccggctac gtgaagagtg atctgctgcc gcgcgtgcgc tacttcgcct acgtcgacat
     4801 accctcgaat gcgtccgggg gctggttttc gcggccgtcg gacctgtggc agtgttacga
     4861 agcggtttcc gacgacagct tcgccaagcg caaggtgcac gatcggcgcg acatctggcc
     4921 ggtgttccga gatttgttcg cgcgccggga ggccgtggca tgaacccttg ggatgccgcc
     4981 ttcaagggcg atcagccagt cgccgagcgg ccgtggccga agctgctgtc gaagccgacg
     5041 gtgccgatgc cgcgcgccga gcgtccgcga cgtgagccga tcgccaccgg caccgaatgg
     5101 accgtgccgc tactggagcg cttcgacacg gcgatcgccg ggcatgccaa gcgattgggg
     5161 ttggtgacgt ttccgatcca gtacgaggtg atcactgccg atcagatgct cgacctgtgc
     5221 tcgaccgtcg gcatgccggt gcactacccg cactggagct tcggcaagca gttggtaagc
     5281 caggagcgcg gctatcgcaa gggccgcgct ggactggcgt acgaaatcgt gatcaacacc
     5341 aacccgtcgc tcgtctacct gatggaaacg aacacgctgg cgctgcaggt gctggtgatg
     5401 gcgcacgccg cgtacgggca caacgcattc ttccgcaaca actacctgtt ccagcagttc
     5461 acgcaaccgg acgcgattct cgactacctg aagttcgcgc gcggctatat cgtcgagtgc
     5521 gagcagcgct acgactggcg tgatgtcgag aaggtactcg actgctgcca cgcgatcgcg
     5581 ccctacggcg tcgatcgcta ccgtcggccg gggcgcttgt ctgcgaaacg cgagcgcgag
     5641 cggcaggtcg agcggctgcg ctttgcggag cggcacttca atccggacta tgcgcacatg
     5701 tacgccgaac gcaaggaaga gccgagtgcg ccggcgttcg aaccgcagga gaacctgctt
     5761 tacttcatcg aaaagtcggc gcccaagctc gccccctggc agcgcgaact ggtgcgcatc
     5821 gtgcgcaagg tgtcgcagta cttctacccg cagcgactga ccaaggtcgc gaatgaaggc
     5881 gcggcgacct tctggcacta cacgctgctg cacgagctgt acgacgccgg ccaggtcgac
     5941 gacggcttca tgctcgagtg gctggccaac cacagcgacg tggtcacgca gctgccgttc
     6001 gactcgcagc actaccacgg catcaatccc tacgccctcg gcttcgcaat catgcgcgac
     6061 attcgccgca tctgcgagca gccgaccgac gaggaccggc agtggttccc gcacctggcc
     6121 ggccgcgaat ggaccgagca gatccatttc gcgatggcga acttcaagga cgagagcctg
     6181 atcgagcaat acctgtcgcc gaaggtcatg cgcgacatgc ggctgttcat gctggcggac
     6241 atggagcagt cgcgcgacga atatcgcgtc gaggcgatcc acaacgagca gggctacctg
     6301 cgcgtgcgac agactctggc cgcgcaatac cgtccagagc attacctgcc gcagatcgtg
     6361 gtcgctcgcg acagcgcgga ctcggaccgt gcgctgctgc tgcagcatcg cgtcgagcac
     6421 ggccggctgc tcgaagccga agcagtcgcc gaggtgctcg gctatgtcaa ggaactgtgg
     6481 cagttcgacg tcgtcttgga agagatcgac gacgccggca accggctgaa gatccaccga
     6541 acctagctcc gataacggat caggttatcg gcacgagcct cgatccgagt gtcgggcaa
//
LOCUS       DBNHBY010000144         4175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1428777, whole genome shotgun sequence.
ACCESSION   DBNHBY010000144 DBNHBY010000000
VERSION     DBNHBY010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1428777"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..657
                     /locus_tag="AC16HB_03445"
     CDS             211..657
                     /locus_tag="AC16HB_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR5605874.1"
                     /translation="MNATVHPLLARLSAALDAPILPVEQFDAWAAQPGHALVVFTEDP
                     VLFRETLDLAVIVPELAQAFPQRFRTAVLLQAAARAIAPRYGFRRWPALVLLKDGRYV
                     GAIDGLRDWQDYLQELQRLLEAQPTRPPSIGIAVTAAGQGDSQPCH"
     gene            671..1525
                     /locus_tag="AC16HB_03450"
     CDS             671..1525
                     /locus_tag="AC16HB_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HIR5605875.1"
                     /translation="MKDFPIPVRGAGSQPEDDGFSYMSIPREVPTFSMPRVPENAEAA
                     AMAAARDLLTRFVDAMGAGNGPCVDLTSFHADGLKVLNDVLGEGEVSVRIQSRNGATH
                     DIRIQETVFAGVWRERHHDGAGDLLHDYLYAGGIPPIAIDMAQSAGAAALAERGAVPP
                     GAMNAPALLTEIAAQMRAFHPGTRPHVINLTLLPLTPEDTHLIEQRVPPGPVAILSRG
                     FGNCRVTSTGLRNVWRVQYFNSMQTLILNTLEITRVPEVALAAAEDLADSRERLRELI
                     DWIAESAA"
     gene            1540..1758
                     /locus_tag="AC16HB_03455"
     CDS             1540..1758
                     /locus_tag="AC16HB_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR5605876.1"
                     /translation="MAEFAGTCQDDGARIGPATRLECRICWYVYDPARGDPVWQIAPG
                     TPFNDLPAHWSCPNCAAERAQFMVLDDE"
     gene            1751..2257
                     /gene="hybE"
                     /locus_tag="AC16HB_03460"
     CDS             1751..2257
                     /gene="hybE"
                     /locus_tag="AC16HB_03460"
                     /inference="COORDINATES: protein motif:HMM:NF023365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HIR5605877.1"
                     /translation="MSETLQQTPSQRLEAAFRAIHAQRMQGLPFVNERLCVEAVGFRR
                     WEGRWLGVLITPWFMNLVLLPDAPESWEPVPLRADARYEFPAGSFDFIGGHEPACGEF
                     QSCSLFSPVFEFEQQEVARATAEAVLAALFDPAAREGARPPEHNPSPAPVVSRRDVLR
                     GRWGEAKT"
     gene            2406..2585
                     /locus_tag="AC16HB_03465"
     CDS             2406..2585
                     /locus_tag="AC16HB_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605878.1"
                     /translation="MNADLAGELLIRMQLRDERVAAVTIESTRPQVADKLLTGRTAAD
                     AVALVPRVFTICGRS"
     gene            2631..3515
                     /locus_tag="AC16HB_03470"
     CDS             2631..3515
                     /locus_tag="AC16HB_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR5605879.1"
                     /translation="MTATARAQHTRAIEAEMAHEYLWRALIDWPKATGGLAQRDAFAR
                     ARDALAREDLAVNEAIGAVVEREVLAGASGAWYEAARVPAFEIWIARAATPAARMVGA
                     VQRDGPRHGAPRDVRAVPLLPAFEGVAASRLLSALDADGAFERAPMLDGKPCETGATA
                     RLAQHPLVAALNEAYGRSVVTRLAARVTELARLAAGQPAGKPLLGNRKLGEGRGLGWV
                     ETARGLLLHVVELVHDEVKRYRIVAPTEWNFHPRGALPAGLVGVRALNDRELRRRAEW
                     LVQALDPCVTYRLELTRA"
     gene            3545..3877
                     /gene="hypA"
                     /locus_tag="AC16HB_03475"
     CDS             3545..3877
                     /gene="hypA"
                     /locus_tag="AC16HB_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR5605880.1"
                     /translation="MALAEGVLGVIEDAARAQGFASVRTVWLEIGRLAAVEPEALCFS
                     FDVVKRGTVADGARIEIVDVPGAAWCMKCGGTVAIEERGSACPACGSYQLQVCGGEDM
                     RVKELEVV"
     gene            3888..>4175
                     /locus_tag="AC16HB_03480"
     CDS             3888..>4175
                     /locus_tag="AC16HB_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR5605881.1"
                     /translation="MCTTCGCTSGETRIEGQHEEEHAHGAGHEHTDVHTHVHADGTTH
                     SHADHAHGPDAAAHSHAPGLTPARMVQIERDILGQNNALAANNRRFFAEHGI"
BASE COUNT          624 a         1432 c         1545 g          574 t
ORIGIN      
        1 gcgaggacga agcgctgcag accctcgccg cactcgatgc gctcgacgcc gcgctgcgcg
       61 gcgaaaccga tccggccgcg ctcgacgcgg ggttcgcgga cctcgtcggt cgcgagccgc
      121 agctccccga acacctgagg aagatcgcat gagcgcgccg ggccgctccc aagcgctcat
      181 cgcgcagcgt gcagcgcgga gggtagcccc gtgaatgcga ccgtgcaccc gttgctcgcg
      241 cgcctgtcgg ccgcgctcga cgcgccgatc ctgccggtcg agcagttcga tgcgtgggcg
      301 gcgcagcccg gccacgcgct cgtcgtgttc accgaggatc cggtgctgtt tcgcgagacg
      361 ctcgacctgg cggtgatcgt gcccgaactg gcgcaggcgt tcccgcagcg tttccgcacc
      421 gcggtgctgc tgcaggccgc ggcgcgcgcg atcgcgccgc gctacggctt ccgccgctgg
      481 ccggcgctgg tgctgctgaa ggacggcagg tatgtcggcg cgatcgacgg cctgcgcgac
      541 tggcaggact acctgcagga actgcaacgc ctgctcgagg cgcagccgac ccgcccaccg
      601 tcgattggca tcgcggtgac ggccgcgggt cagggcgact cgcaaccatg tcattgaacc
      661 tgggatcacg atgaaggact ttcctattcc cgtgcgcggc gccggctcgc agccggagga
      721 cgacggcttc agctacatgt cgatcccgcg cgaggtgccg acgttctcga tgccgcgcgt
      781 gcccgagaac gcggaagcgg cagcgatggc ggccgcacgc gacctgctga cacgcttcgt
      841 cgatgccatg ggcgccggca atggtccgtg cgtcgacctg acgagctttc atgccgacgg
      901 gctcaaggtg ctcaacgacg tgctcggcga gggcgaagtc agcgtgcgca tccagagccg
      961 caacggcgcg acgcatgaca tccgcattca ggagaccgtg ttcgcgggtg tgtggcgcga
     1021 gcgccatcac gacggtgcgg gtgacctgct gcacgattac ctgtacgcgg gcggcatccc
     1081 gccgatcgca atcgacatgg cgcagagcgc cggcgccgcg gcgctcgccg agcgcggcgc
     1141 agtgccgccg ggcgcgatga acgcgcccgc gctactgacc gagatcgcgg cgcagatgcg
     1201 tgcgtttcat cccggaacac gcccgcacgt gatcaacctg acgctgctgc cgctgacgcc
     1261 ggaggacacg cacctgatcg agcagcgggt gccccccggc ccggtggcga tcctgtcccg
     1321 tggcttcggc aactgccgcg tcacgtcgac cgggctgcgc aacgtgtggc gcgtgcagta
     1381 cttcaacagc atgcagacgc tgatcctgaa cacgctcgag atcacgcgcg tgcccgaggt
     1441 ggcgctcgcc gccgccgagg atctggccga cagccgcgaa cgcctgcgcg agctgatcga
     1501 ctggatcgcc gagtccgccg cctgagcgcc ggagatccga tggcggaatt cgcaggcacg
     1561 tgccaggacg acggtgcccg gatcgggccc gcgacgcggc tcgaatgccg gatctgctgg
     1621 tacgtctacg accccgcgcg cggcgacccg gtgtggcaga tcgcgcctgg cacgccgttc
     1681 aacgatctgc cggcgcactg gtcctgcccg aactgcgcgg ccgagcgcgc gcagttcatg
     1741 gtgctcgacg atgagtgaga ccctgcaaca gacgccgtcg cagcggctcg aggcagcctt
     1801 ccgcgcgatc cacgcgcagc gcatgcaagg cctgccgttc gtcaacgaac ggctctgcgt
     1861 cgaggcggtg ggcttccgcc gctgggaggg ccgctggctg ggcgtgctga tcacgccgtg
     1921 gttcatgaat ctcgtgctgt tgccggatgc gccggagagc tgggagccgg tgccgctgcg
     1981 cgcggacgcg cgctacgaat ttccggccgg cagcttcgac ttcatcggcg gccacgagcc
     2041 cgcgtgcggc gagttccaga gctgctcgct gttctcgccg gtgttcgagt tcgagcagca
     2101 ggaagtcgcg cgcgcgaccg ccgaggcggt gctcgccgcg ctgttcgacc cagcggcgcg
     2161 cgaaggcgcg cgccccccgg agcacaatcc cagccccgca ccagtggtgt cgaggcgcga
     2221 cgtgctgcgc ggccgatggg gagaagcgaa gacatgagcc atcgccgggc cgccccagga
     2281 tggctcggac agcccgtcgc gggacgcgac gggctttcga gccgtccagg cccgcgcagg
     2341 caggcttgga gccgcggctc tcacccacct cggggggcca gcaccgaagg tgcgaagggg
     2401 gcactatgaa cgctgacctg gccggtgaac tgttgattcg catgcagcta cgcgacgagc
     2461 gcgtggccgc ggtgacgatc gaatcgacgc ggccgcaggt cgccgacaag ctgctcaccg
     2521 gccgcaccgc cgccgacgcg gtcgcgctgg tgccccgcgt gttcacgatc tgcggacgtt
     2581 cgtaggcggt cgtttcccag ctcgcggtcg aggccgcgcg cggcgagacg atgaccgcca
     2641 ccgcgcgtgc gcagcacacg cgcgcgatcg aggccgaaat ggcgcacgag tacctgtggc
     2701 gtgcgctgat cgactggccc aaggccaccg gggggctggc gcagcgcgac gcctttgcgc
     2761 gcgcgcgcga tgcgctggcg cgcgaggacc tggcggtcaa cgaggcgatc ggcgctgtgg
     2821 tcgagcgcga agtgctggcc ggcgcgagcg gcgcctggta cgaagccgcg cgcgtgcccg
     2881 ccttcgagat ctggatcgcg cgcgcagcca cgccggccgc gcgcatggtc ggtgccgtgc
     2941 agcgcgacgg gccgcgccat ggcgcgccgc gcgacgtgcg cgccgtgccg ctgctgcccg
     3001 cattcgaagg tgtcgccgcc agccggctgc tgagcgcgct ggacgccgat ggcgcgttcg
     3061 agcgcgcgcc catgctcgac ggcaagccat gcgagaccgg cgcgaccgcg cggcttgccc
     3121 agcacccgct ggtcgcggcg ctgaacgagg cctacggccg ctcggtggtc acgcggctgg
     3181 cagcccgcgt gactgagttg gcgcggctgg cggccgggca gccggcgggc aagccgctgc
     3241 tcggcaaccg caagctcggc gaaggtcgcg ggttgggctg ggtcgagacg gcgcgcggcc
     3301 tgctgctgca cgtggtcgaa ctcgtgcacg acgaggtgaa gcggtaccgc atcgtcgcgc
     3361 cgaccgagtg gaacttccat ccgcgcggcg cgctgccggc tggcctggtc ggcgtgcgcg
     3421 cgttgaacga ccgcgaacta cgccgccgcg ccgaatggct ggtgcaggcg ctcgacccgt
     3481 gcgtgaccta ccggctggag ctgacgcgtg catgagacgg aaggtcggga gcgccatgca
     3541 tgagatggct ttggcggaag gcgtgctcgg cgtgatcgag gacgcagcgc gcgcgcaggg
     3601 gttcgcgagc gtgcggacgg tgtggctgga gatcggccgc ctggccgcgg tcgagcccga
     3661 ggcgctgtgc ttctcgttcg acgtggtcaa gcgcggcacg gtcgccgacg gcgcgcgcat
     3721 cgagatcgtc gacgtgccgg gcgcggcgtg gtgcatgaag tgcggcggca cggtggcgat
     3781 cgaagaacgg ggcagcgcct gccccgcgtg cggcagctac cagctgcagg tgtgcggcgg
     3841 cgaggacatg cgggtcaagg aactggaagt ggtctgagga acagacgatg tgcacgacat
     3901 gcggctgcac tagcggcgaa acacggatcg aaggccagca cgaggaagag cacgcgcacg
     3961 gcgccgggca cgagcacacg gacgtgcaca cgcatgtaca tgctgacggc acgacgcatt
     4021 cgcacgctga ccatgcgcac ggaccagacg cagcggcgca ttcgcacgcg cccggcctga
     4081 cgccggcgcg gatggtgcag atcgagcgcg acatcctcgg tcagaacaac gcgctggccg
     4141 cgaacaatcg gcgcttcttc gccgagcacg gcatc
//
LOCUS       DBNHBY010000145         7296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1835238, whole genome shotgun sequence.
ACCESSION   DBNHBY010000145 DBNHBY010000000
VERSION     DBNHBY010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1835238"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16HB_03485"
     CDS             <1..288
                     /locus_tag="AC16HB_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5605882.1"
                     /translation="RVVALDATRNLLNRLAGVRVSLRLRGAPPEALRARVIEASDGHL
                     SLRLAAASEVEGVLAACRAAGCEIDELEVGHADLEDVFLQLMADDRQRMAA"
     gene            285..1097
                     /locus_tag="AC16HB_03490"
     CDS             285..1097
                     /locus_tag="AC16HB_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605883.1"
                     /translation="MNAVQANRIGLDRAQTTASTSGFRTLLYKEVLRFWKVSFQTIAA
                     PVLTGVLYLLVFGHVLEKHVEAFPGVSYTAFLIPGLVMMSVLQNAFANSSSSLIQSKI
                     TGNLVFVLLPPLSNAEFFAAYVIASIVRGLVVGVGVFAVTLWFAPLTYLAPLWIAAFA
                     LAGSALLGVLGLIAGIVSDKFDQLAAFQNFIIMPATFLSGVFYSVKGLPPFWQSVSHF
                     NPFFYIVDGFRYGFFAQADVSPWLSLAVVVVALALVSTLALAMLERGYKIRH"
     gene            1097..1342
                     /locus_tag="AC16HB_03495"
     CDS             1097..1342
                     /locus_tag="AC16HB_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR5605884.1"
                     /translation="MEPTADDVRRYIAAGLDCEMLQVEGDGRHFSALIVARAFEGRNR
                     VQRHQLVYAALGERMRAEVHALSMRTLTPVEYARERK"
     gene            1530..2792
                     /gene="murA"
                     /locus_tag="AC16HB_03500"
     CDS             1530..2792
                     /gene="murA"
                     /locus_tag="AC16HB_03500"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR5605885.1"
                     /translation="MDKLRIAGGMPLRGEIAVAGAKNAALPIMAAGLLTKDALRIANV
                     PQLADIATMAKLTRGMGARIEREPGALTIQATDIASTEAPYELVKTMRAAVLVLGPLV
                     ARFGRARVSLPGGCAIGARPVDQHIKGLQALGADIRVEHGYLIAAAPRLQGARIVTDM
                     VTVTGTENLMMAACLASGETIIENAAREPEVVDLANCLNAMGARVRGAGTDRIVIDGV
                     RELHGTEHRVMPDRIESGTFLVAAAATGQFGGADVRLAGAAPDTLDAVLGKLREAGAQ
                     VDIESQAIRVRMNAPPTAVSLRTAPYPAFPTDMQAQFMALDCMAHGVGRITETIFENR
                     FMHVLELQRLGAKIDIEGNTAIVRGVPRLSGARVMATDLRASASLVIAGLMADGETLV
                     DRIYHLDRGYDRMETRLARLGARIERVK"
     gene            2789..3427
                     /gene="hisG"
                     /locus_tag="AC16HB_03505"
     CDS             2789..3427
                     /gene="hisG"
                     /locus_tag="AC16HB_03505"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR5605886.1"
                     /translation="MITLALSKGRIFDETAPLLARAGITPLENPEQSRKLIIGTSHPG
                     VRIVVVRATDVPTYVQYGAAELGVAGKDVLMEHGGGGLYQPLDLGIANCRMCVAVAAG
                     FDYASAVRRGARLRVATKYVATAREHFASKGVHVDVIKLYGSMELAPLAGLADAIVDL
                     VSSGGTLRANNLVEVEEIASISSRLIVNQAALKLKREEVMPVIDRLEAALNR"
     gene            3424..4722
                     /gene="hisD"
                     /locus_tag="AC16HB_03510"
     CDS             3424..4722
                     /gene="hisD"
                     /locus_tag="AC16HB_03510"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR5605887.1"
                     /translation="MKMRRLDTRAADFDAAFAQLVAHDSAIDRAIGRAAEAIVDDVRT
                     RGDSAVLEYTNRFDRMSAASMSALEVSAAEMRAALDALPTGQRVALEAAARRIESFHE
                     KQRAESWTITEADGTELGQRVNALDSVGVYVPGGLAAYPSSVLMNVVPAQVAGVKDIV
                     MVSPTPGGERNPLVLAAASLAGVTRAFAVGGAQAVAALAYGTAAVPRVDKIVGPGNAY
                     VAAAKRCVFGVVGIDMIAGPSEILVICDGATDPDWIAMDLFSQAEHDELAQSILLTPD
                     ADFIGRVQESIANLLPTMPRREIIEKSLANRGALILTRDLEEACAVADRIAPEHLEIS
                     TDAPERWAAKIRHAGAIFLGRYTSEALGDYCAGPNHVLPTARTARFSSPLGVYDFQKR
                     TSIIRVSRAGAQSLGRIASTLAQGERLPAHAQSAEYRLDR"
     gene            4719..5789
                     /gene="hisC"
                     /locus_tag="AC16HB_03515"
     CDS             4719..5789
                     /gene="hisC"
                     /locus_tag="AC16HB_03515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR5605888.1"
                     /translation="MSRPDLSIIRDDVRAISAYHVPPAAGFVKLDAMENPFGLPAALA
                     HELGARLAQVALNRYPLSDPQAFKAKLAHAIGLPAGMQLLLGNGSDELIHLVIQACAK
                     PGTTVLSPWPSFVMYEMSARFDGCRFVGVPLRPDFALDAGAMRAAIAREQPAVVFIAW
                     PNNPTGNLFDRAEVAAILDAAPGLVVIDEAYLPFAQDTWITQLLSRPNLLVLRTLSKL
                     GLAGIRLGYLAGDPAWLEQFDKVRPPYNVNVLTLAAAEFMLDQLRLLDEQAATIRTER
                     TRMLGSLRRLPGVTAFDSAANFILFRVSDPDGVFARLKERGILIKNVSRMHPLLAGCL
                     RATVGTPAENDAFIEALETSLS"
     gene            5794..6387
                     /gene="hisB"
                     /locus_tag="AC16HB_03520"
     CDS             5794..6387
                     /gene="hisB"
                     /locus_tag="AC16HB_03520"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR5605889.1"
                     /translation="MTARSAEVVRNTKETQIRVVVDLDGSGRSSLATGIGFFDHMLDQ
                     LARHGALDLTIEAQGDLHIDGHHTVEDVGITLGQAVAKAVGDRVGIRRYGHAYVPLDE
                     ALSRVVVDFSGRPGLEWHVPFSRAMIGEFDVDLVHEFFQGFVNHALVTLHVDNLRGDN
                     AHHQCETVFKAFARALRMALEPDPRAAGVVPSTKGTL"
     gene            6444..7088
                     /gene="hisH"
                     /locus_tag="AC16HB_03525"
     CDS             6444..7088
                     /gene="hisH"
                     /locus_tag="AC16HB_03525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR5605890.1"
                     /translation="MIAVIDYGMGNLRSVAKALEHVAPKDAVVVTSDAAVVERAARIV
                     FPGQGAMPDCMRYLHEAGLEEPVRRALRSKPVLAVCIGEQMLFDYSEETRSPGATTPG
                     LRLFPGAVVRFRDADMQHNGLRLKVPHMGWNRVRQTRPHRLWDGIADGAFFYFVHSYY
                     VAPSDAQLTVGQTDYGVSFTCAVARDNIFATQFHPEKSAAAGLRLYENFTRWNP"
     gene            7170..>7296
                     /locus_tag="AC16HB_03530"
     CDS             7170..>7296
                     /locus_tag="AC16HB_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR5605891.1"
                     /translation="MLLIPAIDLKDGKCVRLRQGDMKDVTVFSEDPVAMARHWVAQ"
BASE COUNT         1143 a         2436 c         2528 g         1189 t
ORIGIN      
        1 cgcgtcgttg cactcgatgc gacgcggaac ctgctgaacc ggctcgccgg cgtgcgcgtc
       61 agcctgcgtt tgcgcggcgc gccgcccgag gcactgcgcg cgcgcgtgat cgaggccagc
      121 gacggccacc tctcgctgcg gctcgctgcg gcgtcggagg tcgaaggcgt gctcgccgcg
      181 tgccgcgccg cgggttgcga gatcgacgaa ctcgaagtcg ggcacgcgga cctggaggac
      241 gtgttcctgc aactgatggc ggacgaccgc cagcggatgg cggcatgaac gcggtgcaag
      301 ccaaccggat cgggttggac agggcgcaga cgacggcctc gacctccggc ttccgcacgc
      361 tgctgtacaa ggaagttctg cgcttctgga aggtgagctt ccagaccatc gcggcgcccg
      421 tgctgaccgg cgtgctgtac ctgctggtct tcggtcatgt gctggaaaag cacgtcgagg
      481 cgtttcccgg cgtcagttac accgcgttcc tgatccccgg gctggtgatg atgagcgtac
      541 tgcagaacgc gttcgccaac agctcgtcgt cgctgatcca gtcgaagatc accggcaacc
      601 tggtgttcgt gctgctgccg ccgctgtcga acgcggaatt cttcgctgcg tacgtgatcg
      661 cgtcgatcgt gcgcgggctg gtggtcggcg tcggcgtgtt cgcggtcacg ctctggttcg
      721 cgccgctcac gtatctcgcg ccgttgtgga tcgcggcgtt cgcgctggcc ggcagcgcgc
      781 tgctcggcgt gctcgggctc attgcgggca tcgtgtcgga caagttcgac cagctcgccg
      841 cgttccagaa cttcatcatc atgccggcca cgttcctgtc cggcgtgttc tattcggtca
      901 aggggctgcc gccgttctgg caatccgttt cgcacttcaa cccgttcttc tacatcgtcg
      961 atggttttcg ctacgggttc ttcgcgcagg ccgacgtgtc gccgtggctg agcctcgccg
     1021 tcgtggtcgt cgcgctggcg ctggtgtcca cgcttgcgct ggcgatgctc gagcgtggct
     1081 acaagatcag gcactgatgg aaccgactgc cgacgacgtc aggcgctaca tcgccgccgg
     1141 gctcgactgt gagatgctgc aggtcgaagg cgatggccgt cacttcagcg cgctgatcgt
     1201 cgcgcgcgcg ttcgagggca ggaaccgcgt gcagcggcac cagctcgtgt acgccgcgct
     1261 cggcgagcgg atgcgggcgg aggtgcacgc gctgtcgatg cgcacgctga ccccggtcga
     1321 gtacgcgcgg gagcggaaat gacgcaagct tcacgccgac ttcacattcg cttggcgccc
     1381 gcctcaccgc gcgcggcgat cgttcttccc ggcggaccgc agcccgcggc ccgcgctgac
     1441 cgggggagac cgacatgccc gattcgccgc gcatcaccgc cgccgtgcgc ctgcgctacc
     1501 cgcagcacgc ccgagccacg gagcccgcaa tggacaagct gaggatcgcc ggcggcatgc
     1561 cgctgcgcgg cgagatcgca gtcgccggcg ccaagaacgc ggcgctgccg atcatggccg
     1621 cggggctgct gacgaaggat gcgctgcgga tcgccaacgt gccgcagctc gccgacatcg
     1681 cgacgatggc caagctcacg cgtggcatgg gcgcgcgcat cgagcgcgag cccggcgcgc
     1741 tgacgatcca ggcgacggac atcgcctcga ctgaagcccc gtacgagctc gtcaagacga
     1801 tgcgcgccgc ggtgctggta ctgggacccc tggtggcgcg cttcggccgc gctcgtgtgt
     1861 cactgccggg tggctgcgcg atcggcgcac ggcccgtcga tcagcacatc aaagggctgc
     1921 aggcgctcgg cgccgacatc cgcgtcgagc acggctatct gatcgcggcc gcgccgcggc
     1981 tgcagggcgc ccgcatcgtc acggacatgg tgaccgtcac gggcactgag aacctgatga
     2041 tggccgcatg cttggcctcc ggcgaaacga tcatcgaaaa cgccgcgcgc gagcccgagg
     2101 tggtagacct cgcgaactgc ctgaacgcga tgggcgcgcg cgtgcgcggc gccggcaccg
     2161 accgcatcgt gatcgacggg gtacgcgaac tgcatggcac ggaacaccgc gtgatgccgg
     2221 accggatcga aagcggtacc ttcctggtcg ccgcggcggc gaccgggcag ttcggcggcg
     2281 cggacgtgcg actggcgggc gccgcgcccg acacgctcga cgcggtcctc ggcaagctgc
     2341 gcgaagccgg cgcgcaggtc gacatcgaat cgcaggcgat ccgcgtccgg atgaacgcac
     2401 cgccgacggc ggtcagcctg cgtaccgcgc cgtatccggc gtttccgacc gacatgcagg
     2461 ctcagttcat ggcgctcgac tgcatggcgc acggcgtcgg ccggatcaca gagacgatct
     2521 tcgagaatcg cttcatgcac gtgctcgaac tgcagcggct cggcgcgaag atcgacatcg
     2581 aaggcaacac cgcgatcgtg cgcggcgtgc cgcggctgtc cggggcgcgc gtgatggcga
     2641 ccgacctgcg tgcctcggca agcctcgtga tcgccggcct gatggctgac ggcgaaacgc
     2701 tggtcgatcg catctaccat ctggatcgcg gttacgatcg gatggaaacg cgcctcgctc
     2761 gactcggcgc gcgcatcgaa cgagtcaaat gatcacgctg gcactgtcca agggaaggat
     2821 cttcgacgaa accgcgccgc tgctcgcgcg cgcgggcatc acaccgctgg agaatccgga
     2881 gcagtcgcgc aagctgatca tcggcacctc gcatccgggc gtgcggatcg tcgtggtgcg
     2941 cgccaccgac gtgccgacct acgtgcagta cggcgcggcc gaactcggtg tcgccggcaa
     3001 ggacgtgctg atggagcacg gcggcggcgg actctatcag ccgctcgacc tgggcatcgc
     3061 caactgccgg atgtgcgtgg cggtggcggc gggtttcgat tacgcgagcg cggtgcgtcg
     3121 cggcgcgcgg ttgcgtgtgg cgaccaagta cgtggccacg gcacgggagc atttcgcgtc
     3181 gaaaggcgtg cacgtcgacg tgatcaagct gtacggctcg atggaactgg cgccgctcgc
     3241 gggactggcc gacgcgatcg tcgatctggt gtcgagcggt ggcacgctgc gcgcgaacaa
     3301 cctggtcgag gtcgaggaga tcgcgtcgat cagctcgcgc ctgatcgtca accaggcagc
     3361 gctgaagctc aagcgcgaag aagtgatgcc ggtgatcgac cggctcgagg cggcgttgaa
     3421 tagatgaaga tgcggcgact cgacacccgc gcggcggatt tcgacgctgc gttcgcgcaa
     3481 ctggtcgcgc acgattcggc gattgaccgc gccatcgggc gtgcggcaga ggcgatcgtc
     3541 gacgacgtgc gcacgcgcgg cgacagcgcc gtgctggagt acaccaatcg cttcgaccgc
     3601 atgtccgccg cttcgatgtc cgcgctggag gtttccgctg ccgagatgcg tgccgcactc
     3661 gacgcgctgc cgaccgggca gcgcgtggca ctggaggccg ctgcgcgccg catcgagtcc
     3721 ttccacgaga agcagcgggc ggagtcgtgg acgatcacgg aagcagacgg caccgaactc
     3781 gggcagcgcg tcaacgcgct cgacagcgtc ggcgtgtacg tgccgggcgg gctggcggcg
     3841 tatccgtcgt cggtgctgat gaacgtggtg ccggcgcagg tcgccggcgt caaggacatc
     3901 gtgatggtgt cgcccacgcc aggcggtgag cgcaacccgc tggtgctggc ggccgccagc
     3961 ctggccggcg tcacgcgcgc gttcgcggtc ggcggcgcac aggcagtcgc cgcgctcgcg
     4021 tacggcaccg cggccgtgcc gcgcgtcgac aagatcgttg gccccggcaa cgcgtacgtc
     4081 gcggccgcca agcgctgcgt gtttggcgtg gtcggcatcg acatgatcgc cggcccgagc
     4141 gagatcctgg tgatctgcga cggcgcgacc gatcccgact ggatcgcgat ggatctgttt
     4201 tcacaggccg agcatgacga actggcccag tcgatcctgc tgacgcccga cgcggatttc
     4261 atcggccgcg tccaggaatc gatcgcaaat ctgctgccga cgatgccgcg gcgggagatc
     4321 atcgagaaga gcctcgccaa ccgcggcgcg ctgatcctca cgcgcgatct cgaagaagcc
     4381 tgcgcggtcg ccgaccgcat agcgcccgag cacctcgaga tctcgaccga cgcccccgag
     4441 cgctgggcgg cgaagatacg ccacgcgggc gcgatcttcc tcggccgcta cacgagcgag
     4501 gcgctcggcg actactgcgc gggcccgaac cacgtgttgc cgaccgcgcg caccgcccgc
     4561 ttctcgtcgc cgctcggtgt gtatgacttt cagaagcgca ccagcatcat ccgcgtgtcg
     4621 cgtgcgggcg cgcaatccct cggccgcatc gcctccacgc ttgcccaggg cgagcggttg
     4681 ccggcacacg cgcaaagcgc cgaatatcgg ctcgatcgat gagtcgcccc gatctgtcga
     4741 tcatccgcga cgacgtgcgc gcgatcagcg cgtaccacgt tccgccggcg gcgggcttcg
     4801 tcaagctcga cgcgatggag aatccattcg gcttgccggc cgcgctggcg cacgaactcg
     4861 gcgcgcggct ggcacaggtg gcgctgaacc gctatccgct gtcggatccg caggcgttca
     4921 aggccaagct cgcgcacgcg atcgggctgc cggccggcat gcagctgctg ctcggcaacg
     4981 gctctgacga actgatccat ctcgtgattc aggcctgcgc gaagccgggc acgaccgtgc
     5041 tgtcgccgtg gccgagcttc gtgatgtacg agatgagcgc gcgcttcgat ggctgccgct
     5101 tcgtcggcgt gccgctgcgg ccggacttcg cgctcgatgc cggcgcgatg cgggcggcca
     5161 tcgcgcgcga gcagccggcc gtggtgttca tcgcgtggcc caacaatccc accgggaacc
     5221 tcttcgatcg cgcggaggtc gcggcgattc tcgacgccgc gccggggctg gtcgtgatcg
     5281 acgaggcgta cctgccgttc gcgcaggaca cctggatcac acagctgctg tcgcgtccga
     5341 acctgctggt actgcgcacg ctgtcgaagc tcgggctggc ggggatccgg ctcggctacc
     5401 tcgccggcga tcccgcgtgg ctggagcaat tcgacaaggt gcgaccgccc tacaacgtga
     5461 acgtgctgac gctggcggcg gcagagttca tgctcgacca gctgcgactc ctcgacgagc
     5521 aggcggcgac catccgcacc gaacgaacgc gcatgctggg gtcgctgcgc cgattgcccg
     5581 gcgtaaccgc gttcgattcg gccgccaact tcatcctgtt ccgggtgtcc gatccggacg
     5641 gcgtgtttgc gcggctgaag gagcgcggca tactgatcaa gaatgtctcg cgcatgcatc
     5701 cgctgctcgc cggctgcctg cgcgccacgg tcgggacgcc ggccgagaac gacgcgttca
     5761 tcgaagccct cgagacgagc ctgagctgaa gccatgaccg cgcgcagcgc cgaagtcgtc
     5821 cgcaacacca aggaaacgca gatccgcgtc gtcgtcgatc tcgacggcag cgggcggagc
     5881 tctctcgcga ccgggatcgg cttcttcgac cacatgctcg atcagctggc gcggcacggc
     5941 gcgctggatc tgacgatcga ggctcagggc gacctgcaca tcgacgggca tcatacggtg
     6001 gaggacgtcg gcatcacgct cggtcaggcc gtggcgaaag cggtcggcga cagggtgggc
     6061 atccggcgct acggtcacgc gtacgtgccg ctcgacgagg ctttgtcccg cgtggtggtc
     6121 gatttttccg ggcggccggg actcgaatgg cacgtgccgt tctcgcgcgc gatgatcggc
     6181 gagttcgacg tcgacctcgt gcacgaattc ttccagggct tcgtcaacca cgcgctggtc
     6241 acgctgcacg tcgacaacct gcgcggcgac aacgcgcacc atcagtgcga gaccgtgttc
     6301 aaggcctttg ctcgcgcgct gcggatggca ctggaaccgg acccacgcgc ggcgggtgtc
     6361 gtgccctcca cgaaagggac cttgtgaccc gtagccggtg acccgacacg ggcgtcacga
     6421 gtcgcgaacc acgagtctcg agcatgatcg cagtcatcga ctacggcatg ggcaacctgc
     6481 gttcggtggc gaaggcgctg gagcacgtcg caccgaagga tgcggtcgtc gtgaccagcg
     6541 acgcggcagt ggtcgagcgc gcggcgcgga tcgtctttcc cggacagggc gcgatgccgg
     6601 actgcatgcg ctacctgcac gaggccggcc tggaagaacc ggtccggcgt gcgctgcgga
     6661 gcaagcccgt actcgcagtc tgcatcggcg agcagatgct gttcgactac tccgaggaga
     6721 cgcgctctcc cggtgcgact acgccggggt tgagactgtt tccgggtgcg gtggtgcgct
     6781 tccgcgacgc cgacatgcag cacaacggct tacggctgaa ggtgccgcac atgggctgga
     6841 atcgcgtgcg acagacgcgg ccgcatcggc tttgggatgg gatcgccgac ggcgcgttct
     6901 tttacttcgt gcacagctac tacgtcgcgc cgtccgacgc gcagttgacg gtcggccaga
     6961 cggactacgg cgtcagcttt acctgtgcgg tggcgcggga taatattttc gccacccagt
     7021 tccatcccga aaagagcgct gccgccggac tgaggctcta cgagaacttc acgcgctgga
     7081 atccttgatt ccggcgggac cggcatcttc cccccatcct ccccgcccgc agcgacgttt
     7141 tcgagccgcg gctcttctcc aggaagtcaa tgctgctgat tcccgccatc gatctgaaag
     7201 acggcaaatg cgtgcgcctg cgtcagggcg acatgaagga cgtcaccgtc ttttcggagg
     7261 acccggtggc gatggcgcgg cactgggtcg cacagg
//
LOCUS       DBNHBY010000146        14464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1497174, whole genome shotgun sequence.
ACCESSION   DBNHBY010000146 DBNHBY010000000
VERSION     DBNHBY010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1497174"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="truB"
                     /locus_tag="AC16HB_03535"
     CDS             complement(<1..871)
                     /gene="truB"
                     /locus_tag="AC16HB_03535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR5605892.1"
                     /translation="MARARRGDPVDGVLLLDKPSGITSNLALQTARRLLNAAKAGHCG
                     TLDPLASGLLPLTFGEATKFSADLLDADKAYEARLQFGVRTATGDAEGEVIETRAVAV
                     TRDAVEAVLARFIGTIEQVPPMHSALKHQGRPLYALARRGIEVARVPRAVRIGRLRLL
                     EFDGDRARIAVECSKGTYVRVLAEDIGAALGCGAHLAALRRTRVGALTLEHAVELAAL
                     ETMPPAARREWLLPADALLASLARIDLNEADAARLAHGQAVATRQAESPRVRVYGAAG
                     LLGVASIDAHGVLA"
     gene            complement(875..1243)
                     /gene="rbfA"
                     /locus_tag="AC16HB_03540"
     CDS             complement(875..1243)
                     /gene="rbfA"
                     /locus_tag="AC16HB_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR5605893.1"
                     /translation="MKPSKNPSRGLRIADQIQRDLGELIPREVRDPRVGLVTVSGVEV
                     SPDYAHAKVFFTVIGSDPEVAAHGLNAAAGHLHNLLFKRLHIHTVPRLHFVHDASVER
                     GFEIDRLIDRAVGKPEHPNE"
     gene            complement(1258..3966)
                     /gene="infB"
                     /locus_tag="AC16HB_03545"
     CDS             complement(1258..3966)
                     /gene="infB"
                     /locus_tag="AC16HB_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5605894.1"
                     /translation="MASNTVAQFAAELKLLPPMLLEQLRAAGVNKTAESDVLSEDDKA
                     RLLESLRRAHGGEAAEKRKITLTRKQTSEIKQADATGKARTIQVEVRKKRVFVKRDAE
                     GEAEAVPAPAAAPVIDEAELRKREEEARRQAELAARQTAEARERAELAQREVVEAQRK
                     EQQELEAARAREASAQAELERVKAEQVAADKVAKEAQLAQERARRKAEEEVAAIKTMM
                     AAPRKVVKAPAPEGTLHKPVAKVEAKPAEAKKDEKKPGVKEVKSGKLASSWSEDAAKK
                     RALKTRGDTGGAGGWRGPKGRRAGRGEEERAPVAMPSEPIVRDVSVSETISVADLAHK
                     MAFKASEVIKVLMKMGQMVTINHVLDQETAMIVVEEMGHRALAAKLDSPESLLAETEA
                     AGASEAVAETRPPVVTIMGHVDHGKTSLLDYIRRTKVAAGEAGGITQHIGAYHVETPR
                     GIITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAAGVPIVV
                     ALNKIDKPEANPERVKQELVANEVVPEEYGGDSPFVPVSAKTGKGIEELLENVLLQAE
                     VKQLTAVKDAPAKGLIIESKLDKGRGPVATVLVQAGTLKRGDVVLAGSAFGRVRAMLD
                     EAGKSIPDAGPSIPVEIQGLSDVPAAGDELIVLGDERKAREIALYRQGKFRDTKLAKQ
                     QAAKLENMFEQMGEGAARTLPLIIKADVQGSQEALVHALTKLSTDEVKVQVVHAQVGG
                     ITESDVNLALASKAVIIGFNVRADQSARKLAESNGIQIRYYNIIYDAVDDVKAALSGL
                     LAPEQREQVLGLVEIRQIFRVPKVGSVAGCMVLDGVVKRTAQARLLRDNVVIWTGELA
                     SLKRFKDDVREVKAGFECGLGLKGYDDIKEGDQLEVFEVQEVARTL"
     gene            complement(4016..5503)
                     /gene="nusA"
                     /locus_tag="AC16HB_03550"
     CDS             complement(4016..5503)
                     /gene="nusA"
                     /locus_tag="AC16HB_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012432652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5605895.1"
                     /translation="MNGREILLTVDVLAREKNVPRDVVFGALEAALAGATKKLFDEED
                     VDIRVAVDRETGDYEAFRRWLVVPDEAGLQEPGHQIILTEAKEQIPDIEVDEYIEEAL
                     PKREETLGRRFAQDTKQAILQKIRDAEREQLLTEFLARGDEIINGTVKRLDKGDLIIE
                     AGKIEARLPRSEMIPKENLRIGDRVRAFVAKIDRQARGPQVILSRTSPEFLKKLFELE
                     VPEIEQGLLQIKSAARDPGVRAKIAVWTNDRRIDPIGTCVGMRGSRVQAVTNELAGER
                     VDIVLWSDDPAQFVIGALAPANVSSILVDEDKHAMDVVVDEDNLAIAIGRSGQNVRLA
                     SELTGWQINIMTAEESAQKKESEQVNVRAMFVEKLDVDEEVANILIQEGFSTLEEIAY
                     VPVSELLEIEAFDEDTIEELRNRARNALLTEAIVREENLEKVDKSLLELEDMDNELAA
                     KLAANNLHTRDDLAELAVDELTEMTGIDDERAKKLIMAARAHWFA"
     gene            complement(5500..6033)
                     /locus_tag="AC16HB_03555"
     CDS             complement(5500..6033)
                     /locus_tag="AC16HB_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR5605896.1"
                     /translation="MGRATAELIALVERTAEGLGYELVDVERQGGLLRVTIDAAAGIR
                     IDDCERLSHQLTHLFAVEGVDYERLEVSSPGLDRPLKRANDFRRFIGAEANFHLFAPV
                     DGRKRLRGRLLEVRGADGQEQIVVQVSEAAVPQPRSPSKRKPAARPAAAPKVVEFALA
                     DVDRARLVPELDFRSGR"
     gene            complement(6261..7355)
                     /gene="rluB"
                     /locus_tag="AC16HB_03560"
     CDS             complement(6261..7355)
                     /gene="rluB"
                     /locus_tag="AC16HB_03560"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF007976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HIR5605897.1"
                     /translation="MGEGDDINMAAMAAMPLPREMLERGRRAAKQTLNAQSEKLHKVL
                     ADAGIGSRRDMEELIIAGRVSVNGEPAHVGQRVMPTDQVRVNGRPLQLRLKGAGRPPR
                     VLLYHKPAGEIVSQDDPEHRPSVFDNLPKVSGGRWVAVGRLDFNTEGLLIFTTSGELA
                     NRLMHPRYEVEREYAVRVLGQLTDEQQQRLRDGVQLEDGLARFARIDDAGGQGANHWY
                     RVTISEGRNREVRRIFETVGLEVSRLIRTRFGAVQLPRTLPRGRFQELSPEWVQAWTH
                     DLGISIDELRSRPQGQGGQGSHGGRRNKPQRQGPPRQPDPMTSTVSYIAAGTHHGRGF
                     GGARGQGYGQGYGQGQGPMRSRRGKPRGFG"
     gene            complement(7781..8281)
                     /gene="scpB"
                     /locus_tag="AC16HB_03565"
                     /pseudo
     CDS             complement(7781..8281)
                     /gene="scpB"
                     /locus_tag="AC16HB_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007728.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
     gene            8726..9370
                     /gene="lexA"
                     /locus_tag="AC16HB_03570"
     CDS             8726..9370
                     /gene="lexA"
                     /locus_tag="AC16HB_03570"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5605898.1"
                     /translation="MAVTDTPAPLTSRQSEILEFIQQALIEHGAPPTRDEIARAFGFR
                     SLNAAEQHLQALARKGLIELVAGTSRGIRVKDAVHALREAAKEFGLPLVGKVAAGSPI
                     LAQENVLKHPAIDPRTFKPRADYLLEVRGMSMRDAGILDGDWLAVHKQAQAQSGQIVV
                     ARLGDDVTVKRLKLRGARAELIAENPDYKPIIVDPRRDAFVIEGIAVGVIRPSV"
     gene            9412..10206
                     /gene="imuA"
                     /locus_tag="AC16HB_03575"
     CDS             9412..10206
                     /gene="imuA"
                     /locus_tag="AC16HB_03575"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HIR5605899.1"
                     /translation="MRAATALASLLNSGAAPIWQASEIAPRDGAASIATGFAALDAEL
                     PGGGWPQGQLVELLLDAPGMGELSLLAPALARMARDRPGVWVLPCDAPGACTLLYPPA
                     LAGASIAVARSIFVQPATPSESLWALEQSLRTAHLGALVGWLPASASRDGDFRALRRL
                     HLLAQRSRALAFLLRETRAAEAASPAALRLRLACADGKLHVTVLKRRGWPLLEPVVLQ
                     VHPAHWNEARVQTQMPTPPDTPERIVVAGAPALPARRWSLQSIFSH"
     gene            10218..10799
                     /locus_tag="AC16HB_03580"
     CDS             10218..10799
                     /locus_tag="AC16HB_03580"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5605900.1"
                     /translation="MASLAHHFLTMAYNNAWANHRLLKACAQLSQADFVATRTSFFPS
                     IKATLNHNVTVDWLYVDMLERSTRGEPLNPAPERFFEPEEPFDACAELAPAQRAVDRR
                     PIDLCAHLSDAQLDGPVQVPRRAGTYTDRVARILAHLFQHQIHHRGQAHAMLAGTPVK
                     PPQLDEFFCSNEGPLRAADFAELGFTEEMIWGA"
     gene            10820..12319
                     /locus_tag="AC16HB_03585"
     CDS             10820..12319
                     /locus_tag="AC16HB_03585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR5605901.1"
                     /translation="MATPLWLAIVLPALPLQLVARALEQPGPVAIVEGPAQRLLVAFC
                     NDVARAAGIAPGLKLAAAQALARELIAIERKLEVERDALHELACWAYQFSAQISLQDN
                     GLLLETGASEVLFGGRIPLHRHIAHGLRELGYTAAFGHAATPRAAWLIGRARTLGIAA
                     HDALQAAQMRDALASLPLRVLDWDERTTDTLHALGLATIGDVLRLPRDAFAKRFGTWL
                     LDDLDRALGCRPDPQPLFAPPELFEAGIELPADVIDAEQLMFPAHRLLRSLEGFLRGR
                     GAGTTELVFTARHSPRRARPLAPTRIALQLATPERDAARLAKLLAERLARVRLPEPAI
                     TLALHVEHLSPFAAVNASLLPPAGGGGTDWLQLAETLHARLGSERVFQLQLVDDHRPE
                     HAYRVVPLAVDADERGVPAQATPQRPLLLVESPRPLACRQETPDYHGPLALVAGPERI
                     ESGWWDIGAPRRHSVHRDYFVARNPRGQTLWVYRELAAPRRWFLHGFFA"
     gene            12353..12610
                     /locus_tag="AC16HB_03590"
     CDS             12353..12610
                     /locus_tag="AC16HB_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HIR5605902.1"
                     /translation="MSTVLRYRNLRLAIYSNDHPPPHVHVLGPDGEARIAIGDDHTPP
                     EIWDFHGMPVRQVITALRQVARHQTELLAAWRKIHGPLDLD"
     gene            12588..13028
                     /locus_tag="AC16HB_03595"
     CDS             12588..13028
                     /locus_tag="AC16HB_03595"
                     /inference="COORDINATES: protein motif:HMM:NF021873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR5605903.1"
                     /translation="MARWTWTDKQLQTQLQKAARAGRLAEKVESRAAAARYVRSSCKL
                     EITLRSGITVAIPIALLPYFDGATERELADVEISALGRGLHWKALDLDLSVPALMAQT
                     LEASTAMAELGRIGGSRTSRVKARAARENGRRGGRPRKREATTV"
     gene            13070..13321
                     /locus_tag="AC16HB_03600"
     CDS             13070..13321
                     /locus_tag="AC16HB_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR5605904.1"
                     /translation="MGTTRVFKVGLRQAVRLPRQCHFDGNEVYIARMGDAVVLLPKPG
                     NWRVLLSALKKLEPDFRIERGWLKILRRRPALARLLKIR"
     gene            13393..>14464
                     /locus_tag="AC16HB_03605"
     CDS             13393..>14464
                     /locus_tag="AC16HB_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5605905.1"
                     /translation="MQVSYAELHCVSNFSFLRGASHPEELVERALVLEYAGLAITDEC
                     SFAGSVRAHLTLTELRENDAAARDFRLIHGTEIQLADGPRLVLLAQTRAGYGNLSQLV
                     TLARRQAVKGSYRLTRADVEAHAHWLDDTLAILVPASAPSAEHVDWLKELAPDRCWIA
                     CELTRGPDDAAHLAQLQALARRTGIPLLAAGDVHIHVRRRKKLQDALTAVRLKTPLAQ
                     CGRRLLPNAERYLRPLYRLARIYPPELLAETLAIAQRCTFSLDELRYEYPEEIVPAGD
                     TPTTWLRKATYEEAKVRYPAGMPDKVRAIVEHELALIEELRYEPYFLTVYDIVKFARR
                     QNILCQGRGSAANSAVCYCLGIT"
BASE COUNT         2116 a         5217 c         4689 g         2442 t
ORIGIN      
        1 gtgcaagcac tccatgggcg tcgatcgacg ccacgccgag cagtcccgcc gcgccgtaca
       61 cgcgcacgcg cggcgactcc gcctgccggg tcgcgaccgc ctggccgtgc gccagccgcg
      121 cggcgtccgc ttcgttcagg tcgatccgcg ccagcgatgc caacaaggca tcggccggaa
      181 gcagccactc gcgccgcgcc gcgggcggca tcgtctccag cgcggccagt tccaccgcgt
      241 gctccagcgt cagcgcgccg acgcgcgtcc tgcgcagcgc ggcaaggtgc gcgccgcaac
      301 cgagcgccgc accgatgtcc tcggccagca cgcgtacgta cgtgcccttg ctgcactcga
      361 ccgcgatgcg cgcgcggtcg ccgtcgaatt ccagcaatcg cagccgcccg atgcgcaccg
      421 cgcgcggcac gcgcgcgact tcgatgcccc gccgcgccag cgcgtacagc ggccggccct
      481 ggtgcttcag cgccgaatgc atcggcggca cctgctcgat cgtgccgatg aagcgcgcca
      541 gcactgcctc gacggcatcg cgcgtgaccg ccaccgcacg cgtttcgatc acctcgccct
      601 cggcatcccc ggtcgccgtg cgcacgccga actgcaacct agcctcgtac gccttgtcgg
      661 cgtcgagcag gtcggccgag aacttcgtcg cctcgccgaa cgtcagcggc agcagcccgc
      721 tggcaagcgg atcgagcgtg ccgcagtgcc ccgccttggc cgcattcagc agccggcgcg
      781 ctgtctgcag ggcgaggttc gacgtgatgc cactcggctt gtcgagcagc agcacgccat
      841 cgaccgggtc accccggcga gcgcgtgcca ccggtcactc gttcgggtgt tcgggcttgc
      901 cgaccgcgcg gtcgatcagc cggtcgatct cgaagccacg ctcgacgctg gcgtcgtgga
      961 cgaagtgcag gcgcggcacg gtgtggatgt gcagccgctt gaacagcagg ttgtgcagat
     1021 gccccgcggc tgcgttcaat ccgtgcgcag cgacttccgg atcgctgccg atcacggtga
     1081 agaacacctt cgcgtgcgcg tagtccggcg agacctcgac gccgctgacc gtcaccagcc
     1141 cgacacgcgg atcgcgcacc tcgcgcggaa tcagttcgcc cagatcgcgc tggatctggt
     1201 cggcgatgcg cagcccgcgg ctcggattct tcgacggttt catgcggctc gcgcgcgtta
     1261 cagcgtgcgc gcgacctcct gcacctcgaa cacctcgagc tggtcgcctt ccttgatgtc
     1321 gtcgtagccc ttcaggccga gtccgcactc gaagcccgcc ttgacctcgc gcacgtcgtc
     1381 cttgaagcgc ttcagcgacg ccagttcgcc ggtccagatc accacgttgt cgcgcagcag
     1441 gcgcgcctgc gcggtgcgct tgacgacccc gtcgagcacc atgcagccgg ccaccgaccc
     1501 gaccttcggc acgcggaaga tctgccggat ctctaccagc ccgagcacct gctcgcgctg
     1561 ctccggcgcc agcagcccgc tcaaggccgc tttcacgtcg tcgaccgcgt cgtagatgat
     1621 gttgtagtag cggatctgga tgccgttgct ctccgccagc ttgcgcgccg attggtcggc
     1681 gcgcacattg aagccgatga tcacggcctt gctcgccagc gcgaggttga cgtcgctctc
     1741 tgtgatgccg cccacctgcg cgtgcacgac ctgcaccttg acctcgtcgg tcgacagttt
     1801 cgtgagcgca tgcaccagcg cttcctgcga cccctgcacg tcggccttga tgatcagcgg
     1861 cagcgtgcgc gcggcgccct ctcccatctg ctcgaacatg ttctccagct tcgccgcctg
     1921 ctgcttggcg agcttggtat cgcggaactt gccctggcgg tacagcgcga tctcgcgcgc
     1981 cttgcgctcg tcgccgagca cgatcagctc gtcaccggcg gccggcacat cggacaggcc
     2041 ctggatctcg accgggatcg acggccccgc gtccggaatg ctcttgccag cctcgtccag
     2101 catcgcgcga acgcggccga acgccgagcc cgcgaggacg acgtcgccgc gcttgagcgt
     2161 cccggcctgc accagcaccg tggctaccgg accacggccc ttgtcgagtt tcgattcgat
     2221 gatgaggccc ttggccggag catcctttac cgccgtcagc tgcttgacct cggcctgcag
     2281 cagtacgttt tccagcaact cttcgatgcc cttgccggtc ttggccgaca ccggcacgaa
     2341 cggagaatca ccaccgtatt cctccggtac gacctcgttg gcaaccagtt cctgcttcac
     2401 tcgctcgggg ttcgcctcgg gcttgtcgat cttgttcagg gcgacaacga tcggaacccc
     2461 ggccgccttc gcgtgcgcga tcgcctcctt ggtctgcggc atcacgccgt cgtcggccgc
     2521 cacgaccagg atgacgatgt cggtcgcctt ggcaccgcgc gcgcgcatcg cggtgaacgc
     2581 ctcgtggccc ggcgtgtcga ggaaggtgat gatcccgcgc ggcgtctcca cgtggtaggc
     2641 gccgatgtgc tgcgtgatgc cgcccgcctc gcccgcggcg accttggtgc ggcgaatgta
     2701 gtcgagcaac gaagtcttgc cgtgatcgac gtggcccatg atcgtgacga ccggcggacg
     2761 cgtctcggcc acggcctccg atgcccccgc cgcctccgtc tccgcaagca gcgactcggg
     2821 ggaatccagc ttggccgcga gcgcgcggtg gcccatctcc tcgaccacga tcatcgcggt
     2881 ttcctggtcg agcacatggt tgatggtcac catctggccc atcttcatca gcaccttgat
     2941 gacctcggag gccttgaagg ccatcttgtg cgcgaggtcg gccaccgaaa tcgtctcgga
     3001 caccgacacg tcgcgcacga tcggttcgct cggcatcgcc accggtgcgc gctcctcttc
     3061 gccgcgcccg gcacggcggc ccttcggacc gcgccagcca ccggcgccgc cggtgtcacc
     3121 gcgtgtcttc agcgcgcgct tcttcgccgc gtcctcggac cacgacgacg cgagcttgcc
     3181 ggacttcact tccttgacgc ctggcttctt ctcgtccttc ttggcctcgg ctggtttggc
     3241 ttcgaccttg gccaccggct tgtgcagtgt gccctccggc gccggcgcct tcacgacctt
     3301 gcgcggcgcg gccatcatgg tcttgatcgc ggcgacttct tcctcggcct tgcggcgcgc
     3361 gcgctcctgt gcgagctgcg cctccttggc aaccttgtcg gccgcgacct gctctgcctt
     3421 cacgcgctcg agttccgcct gcgcactggc ctcgcgcgcg cgcgcggctt ccagctcctg
     3481 ctgttccttg cgctgcgcct ccaccacttc gcgctgtgcg agctcggcgc gctcgcgcgc
     3541 ttcggcggtc tggcgcgcgg caagttcggc ctggcgccgc gcttcttcct cgcgcttgcg
     3601 cagttcagct tcgtcgatca ccggcgcggc ggccggcgcg ggaaccgctt ccgcctcgcc
     3661 ctcggcatcg cgcttgacga acacgcgctt cttgcgcacc tcgacctgga tcgtgcgcgc
     3721 cttgccggtc gcgtcggcct gcttgatctc ggacgtctgc ttgcgtgtca gcgtgatctt
     3781 gcgcttctcg gccgcctcgc cgccgtgtgc gcggcgcagc gactccagca ggcgcgcctt
     3841 gtcgtcctcc gacagcacat cgctctcggc ggtcttgttc acgccggcgg cgcgcagctg
     3901 ttcgagcagc atcggcggca gaagcttcag ttccgcggca aattgggcga cggtattgct
     3961 ggccatggtg tcctcttcag cgtcgggtcg gttgcgccta ggttgcgtga gcggatcagg
     4021 caaaccagtg ggcgcgcgcc gccatgatca gtttcttcgc gcgttcatcg tcgatgccgg
     4081 tcatctcggt cagctcgtcg accgcgagct ccgccaggtc gtcgcgtgtg tgcagattgt
     4141 tggcggccag cttcgccgcc agttcgttgt ccatgtcttc gagctcgagc agcgacttgt
     4201 cgaccttctc gaggttctcc tcgcgcacga tcgcctcggt cagcaatgca ttgcgcgcgc
     4261 ggttacgcag ctcttcgatc gtgtcctcgt cgaaggcctc gatctccagc agctcggaca
     4321 ccggtacgta ggcgatctcc tcgagcgtgg agaagccttc ctggatcagg atattcgcga
     4381 cttcctcgtc gacgtcgagc ttctcgacga acatcgcacg cacgttgacc tgctcggact
     4441 ccttcttctg cgcggactcc tcggcggtca tgatgttgat ctgccagccg gtcagttcgc
     4501 tcgccaggcg cacgttctgc ccgctgcggc cgatcgcgat cgcgaggttg tcctcgtcga
     4561 ccaccacgtc catcgcgtgc ttgtcctcgt cgaccaggat cgacgacacg ttggccgggg
     4621 ccagtgcgcc gatcacgaac tgcgccggat cgtcggacca caacacgatg tcgacccgct
     4681 cgcccgcgag ctcgttggtc accgcctgca cgcgcgagcc gcgcatgccg acgcaggtcc
     4741 cgatcggatc gatgcggcgg tcgttggtcc acaccgcgat cttggcgcgt acgccgggat
     4801 cgcgcgcggc cgacttgatc tgcagcaggc cctgctcgat ctcgggcact tcgagctcga
     4861 acagcttctt caggaactcg ggcgacgtgc gcgacaggat cacctgcggg ccgcgcgcct
     4921 gccggtcgat cttggcgacg aaagcgcgca cgcgatcgcc gatgcgcagg ttctccttcg
     4981 ggatcatctc cgaccggggc agccgcgcct cgatcttgcc ggcctcgatg atcagatcgc
     5041 ccttgtcgag ccgtttgacg gtgccgttga tgatctcgtc gccgcgcgcg aggaactcgg
     5101 tcagcagctg ctcgcgctcc gcatcgcgga tcttctgcag gatcgcttgc ttggtgtcct
     5161 gggcaaagcg acggccgagc gtttcctcgc gcttgggcag cgcttcctcg atgtactcgt
     5221 cgacctcgat gtcggggatc tgctccttgg cttccgtcag gatgatctgg tgcccgggct
     5281 cctgcaatcc ggcctcgtcc ggcacgacga gccagcggcg gaacgcctcg tagtcaccgg
     5341 tctcgcgatc gaccgcgacg cggatatcga cgtcttcctc gtcgaacagc ttcttggtcg
     5401 cgcccgcgag cgcggcttcg agggcgccga acaccacgtc gcgcggcacg ttcttttcac
     5461 gcgccagcac gtcgaccgtc agcagaattt ctcttccgtt catcgcccac tcctgaaatc
     5521 cagttccggc accagccgcg cccgatcgac gtcggccaat gcaaactcaa cgaccttcgg
     5581 cgccgccgcc ggcctggccg ccggcttgcg cttcgaaggc gaacgcggct gcggcaccgc
     5641 cgcctcgctc acctgcacca cgatctgctc ctgcccgtcg gccccgcgca cctcaagcag
     5701 ccggccgcgc agccgcttgc gcccatcgac cggcgcgaac aggtggaagt tcgcctcggc
     5761 gccgatgaag cgcctgaaat cgttcgcccg cttcagcggc cggtccagtc ccggcgagga
     5821 aacctccagc cgctcgtagt cgacgccttc gaccgcaaac aggtgggtga gctggtgact
     5881 caggcgctcg cagtcatcga tgcgtatgcc agcggccgca tcgatcgtca cgcgcagcag
     5941 accgccctgg cgctcgacgt cgaccagctc gtaacccagc ccttcggcgg tccgctcgac
     6001 cagcgcgatc aattccgccg tcgccctgcc cacttcgatc tcccgccgcg ctacgcgcgg
     6061 cgacatccaa aaaaaaatgg gcgtaaagcc cattcctccg ttccaacagg cccgcgcgtt
     6121 cggcgaaacc cgcgagatcc cggaaccgta cgcgctgcgg tcagtagccc gacccggttg
     6181 atgcgatcga acttttccga cagccctgca ttataggggc gcgccgccgc tgtctcaagc
     6241 gcgcgcccgg gaaaagtgct ctaaccgaag ccgcgtggct tgccgcggcg cgagcgcatc
     6301 ggcccctggc cctgtccata gccctggccg tatccctgcc cgcgcgcgcc accgaacccg
     6361 cggccgtgat gcgtgcccgc cgcgatatag ctcaccgtgc tcgtcatcgg atccggttgg
     6421 cgcggcggcc cctgccgttg cggcttgttg cgccgaccgc cgtgactgcc ctgcccgccc
     6481 tggccttggg ggcgcgatcg cagttcatcg atcgaaatgc cgagatcatg ggtccatgcc
     6541 tgcacccact cgggcgacag ctcctgaaag cggccgcgcg gcagcgtgcg cggcagctgg
     6601 actgcaccga agcgcgtgcg gatcaggcga ctgacctcga ggccgaccgt ctcgaagatg
     6661 cgccgcactt cgcgattgcg gccctcgctg atcgtgacgc gataccagtg gttcgctccc
     6721 tggccgccgg cgtcgtcgat gcgcgcaaag cgtgccaggc cgtcctccaa ttgcacgccg
     6781 tcgcgcaacc gctgctgttg ctcgtcggtg agctggccca ggacgcgcac cgcgtattcg
     6841 cgctcgacct cgtagcgcgg gtgcatcagc cgattcgcca gttcgcccga tgtggtgaag
     6901 atcagcaggc cctcggtgtt gaaatccagc cgcccgaccg cgacccagcg cccgcccgac
     6961 accttgggca ggttgtcgaa cacgctgggg cgatgctcgg ggtcgtcctg cgacacgatc
     7021 tcgccggccg gcttgtgata caggagcacg cgcggcggcc gaccagcgcc cttcagccgc
     7081 agttgcagcg gcctgccgtt cacgcgtacc tgatcggtgg gcatcacgcg ctgcccgaca
     7141 tgcgccggct cgccattgac cgacacgcgg ccggcgatga tcagttcttc catgtcgcgg
     7201 cgcgaaccga tgccggcatc ggcaagcacc ttgtgcagct tctccgactg cgcgttcagc
     7261 gtctgcttgg cggcgcgccg cccgcgctcg agcatctcgc gcggcaacgg catggctgcc
     7321 attgcggcca tgttgatgtc gtcgccctca cccaccagcg gcagcggcgc cgtgggggcc
     7381 tcggcggcga ctgcttcgcc ctctgtcggc gctgcatcgg cgcgcggccc tcgacctcta
     7441 ccgcgacgcc ggcgtccgcg cgggccgcgc tgcggacggt cgtccgcctc gccatcggcc
     7501 acgtcgctcg cggcctcggg tgcgctttct gcgggcgctc ccgcggcggc cggcgcggtc
     7561 ggggcggcag cacctcccgt cgcgtcgtcg ggcgacgggc cagggttgtc cggatcttca
     7621 gcggggcgta tcgcgtgcgt catgactggg cttcggaagt ggcggtcacc gtcacggtgt
     7681 ccgcatcgga ttcgggagcg ggggtggcag gccggtccgc ggtggcggcg gcaaactgaa
     7741 gctcgaactc ggtgggcacg gtcgcctcgc tgcccgcggt gagcgccggc agttcttcga
     7801 gcgagcgcag ggaaaggtcg tcgagaaagg cgcgcgtcgt ggccagcagc gcgggccggc
     7861 ccggtgtttc gcggtgaccg atcacctcga cccagccgcg ctcctcgagc gccttgagca
     7921 cctgtgtgga gacggtcacg ccacggatct cctcgatgtc gccgcgcgtc accggctgcc
     7981 ggtgggcgat gatcgccagt gtctcgagca ccgcacgcga atacctgggc ggcttctccg
     8041 ggttcaggcg atcgagatac ggccgcatcg ccggccggct ctggaagcgc caaccggaag
     8101 ccagcgtcac cagctcgatg ccgcggccct gccagtcgcg cttcagctcg tcgagcaata
     8161 cgcgcagggt gtcggcgttc aactcatcag cgaacaactg gcgcagttcc gcaatcggca
     8221 acggctgctg tgccgtcagc aatgcagtct cgaggacgat cttcgcctct tgtgtgttca
     8281 tgcttctgcg ttcaaatggc tgcaggtcaa caatgcgatc gcatcggcac gccggccgcg
     8341 catcgatgcg ccggccgggc tgccaatacc cgccatcaag cacaaagtcc ggacgatcgg
     8401 tccggcaaat caaaacccgg tagttgaggt ctggccgcgc cgcaggcggc gttcagtgcc
     8461 tgcctgcgtt gcgccgtgac agacgattcg caacacggga ccgtccggcg gaagctgccg
     8521 cgggccgcgt cgacatcacg cgcttcatcc tgcggcaatc gacagtcgat ccaggacggt
     8581 caatcgatgg ctgggcagtt tatcacgcag cctcgcgccg gcaagcgttt gtccgcacgc
     8641 gcattcgggc gagtctgcgc gcgccacttg catttccatt cagtactgta taaattaaca
     8701 gtcaaccccg gcacggagag acacgatggc cgtcaccgac acccccgccc cgctcaccag
     8761 ccgccagagc gagatcctgg agttcatcca gcaggcgctg atcgagcacg gcgcgccgcc
     8821 gacgcgcgat gagattgcgc gcgcgttcgg cttccgctcg ctgaacgcgg ccgagcagca
     8881 tctgcaggcg ctggcgcgaa agggtctgat cgagctggtg gccggcacgt cgcgcggcat
     8941 ccgtgtgaag gacgccgtgc acgcgctgcg cgaggcagcc aaggaattcg gcctgccgct
     9001 ggtgggcaag gtggccgccg gcagcccgat cctggcgcag gaaaacgtgc tgaagcaccc
     9061 ggccatcgat ccgcgcacgt tcaagccgcg cgccgattac ctgctcgagg tacgcggcat
     9121 gagcatgcgc gacgccggca tcctcgacgg cgactggctt gccgtgcaca agcaggcgca
     9181 ggcgcagtcg ggacagatcg tggtggcgcg gctcggtgac gacgttaccg tcaagcggct
     9241 aaagctgcgc ggcgcgcgcg ccgaactgat cgccgagaat cccgactaca agccgatcat
     9301 cgtcgacccg cggcgcgacg ccttcgtgat cgagggcatc gcggtgggcg tgatccggcc
     9361 gtcggtgtag cgcacagcaa cttttccccg gttcacactt ttcgaggcgt catgcgtgcc
     9421 gccacggccc ttgcttccct cctgaattcg ggtgcggccc cgatctggca ggccagcgag
     9481 atcgcgccgc gtgacggcgc cgcatcgatc gcgaccggct ttgccgcgct cgacgccgaa
     9541 ctgcccggcg gcggctggcc gcagggccag ctcgtcgaac tgctgctcga tgcaccgggc
     9601 atgggcgagt tgtcgctgct cgcgccggcg ctggcgcgca tggcgcgcga tcgccccggc
     9661 gtgtgggtgc tgccatgcga tgcgcccggc gcgtgcacgc tgctctaccc gcccgcgctc
     9721 gccggagcca gcatcgcggt cgcgcgcagc atcttcgtgc agcccgccac gccgagcgaa
     9781 agcctgtggg cgctcgagca atcgctgcgc accgcgcatc tgggcgcact ggtcggctgg
     9841 ttgccggcgt cggcttcgcg cgatggggat ttccgtgctc tgcgtcggct gcacctgctc
     9901 gcgcagcgca gccgcgcgct ggcgttcctg ctgcgcgaga cgcgcgccgc cgaggcggca
     9961 tcccccgcgg cgctgcgcct gcggctggcg tgcgccgacg gcaaactgca cgtcaccgtg
    10021 ctcaagcgcc gcggctggcc attgctggag ccggtggtgc tgcaggtgca tccggcacac
    10081 tggaacgagg cgcgcgttca gacgcagatg ccgacgccgc ccgatacgcc ggagcgcatc
    10141 gtcgtcgccg gcgcacccgc cttaccggcg cgccggtggt cgttgcagtc gatcttcagc
    10201 cactgagaag gcgcccgatg gcaagcctcg cgcatcactt cctgacgatg gcctacaaca
    10261 acgcgtgggc caaccaccgg ctgctgaagg cgtgcgcgca gctgtcgcag gccgatttcg
    10321 tcgccacgcg cacgagcttc ttcccgtcga tcaaggcgac gctgaaccac aacgtcaccg
    10381 tcgactggct gtacgtcgac atgctcgagc gcagcacgcg cggtgagccg ctcaatccgg
    10441 cacccgaacg cttcttcgag ccggaggagc cgttcgacgc ctgcgccgag ctcgcgcccg
    10501 cgcagcgcgc ggtcgatcgg cgcccgatcg acctgtgcgc ccatctctcc gacgcgcaac
    10561 tggacggccc ggtgcaggtg ccgcgccgcg ccggcacgta caccgatcgc gtggcgcgca
    10621 tcctcgcgca cctgttccag caccagatcc atcaccgcgg ccaggcgcac gcgatgctcg
    10681 ccggcacgcc cgtgaagccg ccccagctcg acgaattctt ctgctccaac gaagggcccc
    10741 tgcgcgcggc cgacttcgcc gagctcggct tcacggaaga aatgatctgg ggcgcctagc
    10801 ccgatgcgcc gcggtcgaca tggcaacacc gctgtggctc gcgatcgtgc ttcccgcgct
    10861 gccgctgcaa ctggtcgcac gcgcgctcga gcagccggga ccggtggcga tcgtcgaagg
    10921 cccggcgcag cggctgctgg tcgctttctg caatgacgtt gcacgcgcgg ccggcatcgc
    10981 gccggggctg aagctcgcgg ccgcgcaggc actcgcgcgc gaactgatcg ccatcgagcg
    11041 caagcttgag gtcgaacgcg atgcgctgca cgaactggca tgctgggcct atcagttcag
    11101 cgcgcagatc tcgttgcagg acaacggact gctgctggaa accggcgcca gcgaagtgct
    11161 cttcggcggc cgcataccgc tgcaccggca cattgcgcac ggtctgcgcg aactcggcta
    11221 cacggccgca ttcggccatg ccgccacgcc gcgcgcggca tggctgatcg ggcgggcgcg
    11281 cacgctcggc atcgcggcac acgatgcgct gcaggcggcg cagatgcgtg acgcgctggc
    11341 gtcgctgccg ttgcgcgtgc tcgactggga cgaacgcacc accgacacgc tgcacgcgct
    11401 gggactggcg acgatcggcg acgtgctgcg gctgccgcgc gacgcgttcg cgaagcgctt
    11461 cggcacgtgg ctcctcgacg atctggaccg cgcgctcggg tgccggcccg acccgcagcc
    11521 gctgttcgcg ccgcccgaat tgttcgaggc aggaatcgag ctgccggccg acgtgatcga
    11581 tgccgagcag ttgatgtttc ccgcgcatcg gctgctgcgt tcgctggagg gattcctgcg
    11641 cggccgcggc gcgggcacga ccgaactcgt cttcaccgct cggcacagcc cacgtcgcgc
    11701 gcggccactg gcgccaacgc ggatcgcgct gcaactggcc acgcccgagc gcgatgcggc
    11761 gcggttggca aaactgctcg ccgagcgcct ggcgcgcgtg cggctgcccg agcccgcgat
    11821 cacgctcgcg ctgcacgtcg agcacctttc gccgttcgcg gcggtcaacg catcgctgct
    11881 gccgccggcc ggaggcggcg gaacggactg gctgcaactg gcggaaacgc tgcacgcgcg
    11941 gctcggctcc gagcgcgtgt tccagctgca gctcgtcgac gaccatcggc ccgagcacgc
    12001 gtaccgcgtc gtgccgctcg cggtcgacgc ggacgagcgc ggcgtgccgg cgcaggccac
    12061 gccgcagcgg ccgctgctgc tggtcgaatc accgcgaccg ctcgcctgca ggcaggaaac
    12121 ccctgactac cacggcccgc tcgccctcgt ggccgggccg gagcgcatcg agtccggctg
    12181 gtgggatatc ggcgcgccgc gccggcacag cgtgcaccgc gactacttcg tcgcccgcaa
    12241 cccgcgcggg cagacgctgt gggtgtaccg cgagctcgct gcgccgcgca ggtggttcct
    12301 gcacggtttt tttgcctgag ccaatgcata aaaacccaac cgttcggtta ttatgagcac
    12361 cgtacttcgc tatcgcaacc tgcgcctcgc gatctattcg aacgatcatc cgccacccca
    12421 cgtgcatgtg ctcggccccg atggcgaggc gcggatcgcg atcggtgacg atcacactcc
    12481 accggagatc tgggactttc atgggatgcc ggtaagacaa gtgatcactg cgttgcgtca
    12541 agttgcaaga catcagaccg aactactcgc cgcttggagg aaaattcatg gcccgctgga
    12601 cctggactga caagcagttg caaacgcaac tgcagaaagc ggcgcgcgcc ggccggctcg
    12661 ccgagaaagt cgagtcgcgc gccgctgcag cgcgctacgt gcgctcgtcg tgcaagctcg
    12721 agatcacgct gcgctccggc atcaccgttg ccatacccat tgcattgcta ccctacttcg
    12781 acggcgcgac agagcgcgag ctcgcggacg tcgaaatatc tgcgctaggc cgcggcctgc
    12841 attggaaagc actcgacctc gatctcagcg taccggcgct gatggcacag acccttgaag
    12901 cgtcaaccgc catggcagaa ctcggccgca tcggcggcag ccgcacgtcg cgcgtcaagg
    12961 cgcgcgccgc acgcgaaaat ggccgcagag gtgggcgtcc acgcaagcgc gaggcgacca
    13021 ccgtctgatg cagcgccctc atgcgtcgct gaaaggaaag gagagcccga tgggtaccac
    13081 gcgagtcttc aaagtcggcc tgcgccaggc ggtgcgtctg ccaaggcagt gtcacttcga
    13141 cggcaacgag gtctacatcg cgcgcatggg cgacgcggtc gtgctgttgc ctaaacccgg
    13201 caattggcgc gtgcttcttt ccgctctcaa gaagctcgaa cccgattttc ggatcgaacg
    13261 cggctggctg aagatactgc ggagacgccc tgcgctggcc cgcctgttga agatccgatg
    13321 atccgcgcga caaagctgcg catcgaagag cggaccgcct gaccgcgagc tgtgcgcgat
    13381 gaccgaacgc ccatgcaggt ctcctatgcc gaactgcatt gcgtgtcgaa cttctcgttc
    13441 ctgcgcggtg cctcgcaccc ggaggaactg gtcgagcgtg cgctggtgct ggagtacgcg
    13501 gggctcgcga tcaccgacga gtgctcgttc gccggttcgg tgcgcgcgca tctgacgctc
    13561 acggagctgc gcgagaacga tgccgccgcg cgcgacttca ggctgatcca cggcaccgag
    13621 atccagcttg ccgacggacc gaggctcgtg ctgctggcgc agacgcgcgc gggctacggc
    13681 aacctgagcc agctcgtcac gctggcgcgg cggcaggcgg tcaagggcag ctaccggctg
    13741 acgcgcgccg acgtcgaggc gcacgcgcac tggctggacg acacgctggc gattctggtt
    13801 ccggcatccg cgccatccgc ggaacacgtc gactggctga aggaactcgc gcccgatcgc
    13861 tgctggatcg catgtgagct gacgcgcggg cccgacgatg ccgcgcacct cgcgcaactg
    13921 caggcgctcg cgcgacgcac cggcatcccg ctcctcgccg ccggcgacgt gcacatccac
    13981 gtacgccggc gcaagaagct gcaggacgcg ctgacggcgg tgcggctgaa gacgccgctc
    14041 gcgcaatgcg gccggcgact gctgccgaac gccgagcggt atctgcggcc gctgtatcga
    14101 ttggcgcgca tctatccacc cgagttgctg gccgagacgc tggcgatcgc acagcgctgc
    14161 acgttttcgc tcgacgagct gcgctacgag tatccggagg agatcgtgcc ggccggcgac
    14221 acgccgacca cctggctgcg caaggcgact tacgaggaag cgaaggtccg ctaccccgcc
    14281 ggcatgccgg acaaggtccg cgcgatcgtc gagcacgaac tcgcgctgat cgaggagctg
    14341 cgctacgagc cgtacttcct caccgtatac gacatcgtca agttcgcgcg ccgccagaac
    14401 atcctgtgcc aggggcgcgg ctcggcggcg aattcggcgg tgtgctactg cctcggcatc
    14461 accg
//
LOCUS       DBNHBY010000147         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_548898, whole genome shotgun sequence.
ACCESSION   DBNHBY010000147 DBNHBY010000000
VERSION     DBNHBY010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_548898"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16HB_03610"
     CDS             <1..294
                     /locus_tag="AC16HB_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR5605906.1"
                     /translation="AIDLAATMLGAKRVGATRLAVIGAAIGTFAGLLLGIPGLILGPF
                     VGAVAGEMLSHGEWRKASQAGVATWMGLVFGTLAKLALVFAMLGIFAFAYFVG"
     gene            complement(857..>1861)
                     /locus_tag="AC16HB_03615"
     CDS             complement(857..>1861)
                     /locus_tag="AC16HB_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5605907.1"
                     /translation="YVVYGPLANGATSLMFEGVPNYPDHSRFWQVCDKHQVTIFYTAP
                     TALRALMREGDEPVKKFSRKSLRLLGTVGEPINPEAWEWYHRVVGDGRCPIVDTWWQT
                     ETGAALISPLPGAIAQKPGSATLPFFGVQPAIVDNDGKILEGACEGNLVLLDSWPGQM
                     RTVFGDHQRYIDTYFRTFPGRYFTGDGAKRDADGYYWITGRVDDVLNVAGHRLGTAEI
                     ESALVAHHLVAEAAVVGCPHEIKGQGVYAYVTLVHDAEPTEELRKELREWVRREIGPI
                     ATPDYIQWAPGLPKTRSGKIMRRILRKIAANEAEQLGDISTLADPSVVAQLVDNRMLR
                     "
BASE COUNT          277 a          609 c          659 g          316 t
ORIGIN      
        1 gccatcgatc tggcggccac gatgctcggt gcgaaacgcg tcggtgcgac gcggctcgcg
       61 gtgatcggcg cggcgatcgg cacgttcgcg gggctgttgc tcggcattcc ggggctgata
      121 ctcgggccgt tcgtcggtgc ggtcgcgggc gaaatgctgt cgcacggcga gtggcgcaag
      181 gcgtcgcagg cgggcgtggc cacgtggatg gggctggtgt tcggcacgct cgcgaagctg
      241 gcgctggtgt tcgcgatgct cgggatcttc gcgttcgcgt acttcgtggg ctgacccggg
      301 cgatcggagg cagcgcgagc cgacgcgacc gcttccagat caggttcgac gaaacacgcc
      361 gccagtacgt ccgtgtaggc tcgcactcgg catcctgcct cgtgacggtt tcgcgaacct
      421 gaccggaagc gatcacgccg gccggtggcg ttgtgcaccg ctgcgcgaaa gacctgaccg
      481 gaagcgatca cgccggccgg tggcgttgtg caccgcttcg cgaaagccct gaccggaagc
      541 gatcacgccg gccggtggcg ttgtgcaccg cttcgcgtaa gacctgaccg gaagcgatcg
      601 cgccggccgg tgacgttgtg caccgcttcg cgaaagacct gaccggaagc gatcacgccg
      661 gcggagcgct agtgcaccac ctccagcggc ggcacgtggg cttcgggtct gtccggcaaa
      721 agcgtcacga ggcaggatgc cgagtgccga gcccatcagg gacgggattc gaaggcgtct
      781 tttgccggac agatcccgcg gctcgcgggt cgcctctgga gggcgacgcc gaagcgccgc
      841 ccccgcgagt ctggcgtcag cgcagcatgc ggttgtcgac cagctgcgcg acgacgctcg
      901 gatcggccag cgtcgagatg tcgcccagct gctcggcttc gttcgccgcg atcttgcgca
      961 ggatgcgccg catgatcttg ccggaacgag tcttcggcag gcctggcgcc cactggatgt
     1021 agtccggcgt ggcgatcggg ccgatctcgc gccgcaccca ctcgcgcagt tccttgcgca
     1081 gttcctccgt gggctcggcg tcgtgcacca gcgtgacgta cgcgtacacg ccctggccct
     1141 tgatctcgtg cgggcaaccg acgaccgcgg cttcggcgac gaggtgatgc gccaccagtg
     1201 cgctctcgat ctccgccgtg ccgagacgat ggccggcgac gttcagcacg tcgtccacgc
     1261 ggcccgtgat ccagtagtag ccgtccgcat cgcgcttggc accgtcgccg gtgaagtagc
     1321 ggccagggaa ggtgcggaag tacgtgtcga tgtagcgctg gtggtcgccg aacaccgtgc
     1381 gcatctgtcc cggccagctg tcgagcagca cgaggttgcc ttcgcaggcg ccctcgagga
     1441 tcttgccgtc gttgtcgacg attgcaggct gcacgccaaa gaacggcagc gtcgccgaac
     1501 caggcttctg tgcaatggcg ccgggcagcg ggctgatgag cgcggcgccg gtctcagtct
     1561 gccaccacgt gtcgacgatc gggcagcgtc cgtcgccgac gacgcggtga taccactccc
     1621 acgcttcggg attgatcggc tcgccgaccg tcccgagcag gcgcagcgac ttgcgtgaga
     1681 acttcttgac cggctcgtcg ccctcgcgca tcagcgctcg cagggccgtg ggcgcggtgt
     1741 agaagatcgt cacctggtgc ttgtcgcaca cctgccagaa gcggctgtgg tccggatagt
     1801 tcggcacgcc ttcgaacatg agcgaggtcg cgccgttggc cagcggaccg tagacgacgt
     1861 a
//
LOCUS       DBNHBY010000148        15492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_616876, whole genome shotgun sequence.
ACCESSION   DBNHBY010000148 DBNHBY010000000
VERSION     DBNHBY010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_616876"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="AC16HB_03620"
     CDS             complement(<1..716)
                     /locus_tag="AC16HB_03620"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5605908.1"
                     /translation="MAQVAVLGAGAWGTAITSVLAQRHHLTLWSHDAAQVRALAATRR
                     NDRYLAGTVLDASITVTPDLAAAVAHARHGLLVLAVPTAALRETVRGLHAETALAPLV
                     WLCKGFERDSAKLAHEIVRDEIASHPSGPLSGPSFAAEVARGLPTALTVAGPAQFCRW
                     VTQIFHGGALRIYSSDDVIGVEVGGAVKNVMAIATGISDALGLGHNARAALITRGLAE
                     IARLGAALGARAEKFLGLAGV"
     gene            complement(732..1217)
                     /gene="secB"
                     /locus_tag="AC16HB_03625"
     CDS             complement(732..1217)
                     /gene="secB"
                     /locus_tag="AC16HB_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR5605909.1"
                     /translation="MAEIVSPQTAPQAVFQLQRIYLKDASLELPHAPQIFLEQTAPQV
                     DVQLEVSNSKVVDGIYEVVVRATTTAKVGDKVLFLVEGKQAGIFEMRGIPNEQFEAIL
                     GIVCPGIIYPYLRANVADLINRTGLPSVHLAEINFEAMYQQRIAQQQQGIATAAPATT
                     Q"
     gene            complement(1285..1545)
                     /gene="grxC"
                     /locus_tag="AC16HB_03630"
     CDS             complement(1285..1545)
                     /gene="grxC"
                     /locus_tag="AC16HB_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIR5605910.1"
                     /translation="MTARVRMYSTAVCPFCVRAEALLKARGVTEIEKIRIDLDAARRD
                     EMIARTGRRTVPQIYIGETHVGGFDDLSALDRAGKLQPLLAA"
     gene            complement(1565..1966)
                     /locus_tag="AC16HB_03635"
     CDS             complement(1565..1966)
                     /locus_tag="AC16HB_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5605911.1"
                     /translation="MRFFIDNIFLIAVAFISGGMLLWPLIRARAAGPSVSTLQATQLI
                     NSRHAQVVDVRSEDDFGKGSLPNAKNIPTARIASRTGELKKDRPVILVCNTGRSAGPA
                     AAQLRAAGFTDVYVLAGGLAAWREAGLPVKA"
     gene            2041..2796
                     /gene="gpmA"
                     /locus_tag="AC16HB_03640"
     CDS             2041..2796
                     /gene="gpmA"
                     /locus_tag="AC16HB_03640"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR5605912.1"
                     /translation="MQKLVLLRHGESQWNLENRFTGWTDVDLSDNGIREARAAGELLQ
                     REGYVFDIAYTSVLKRAIRTLWITLDALDLMWLPVVHSWRLNERHYGALQGLNKAETA
                     AKYGEQQVLTWRRAYAIAPEPLARDDPRWAGNDPRYRHLKPEDIPATECLKDTVARVV
                     PFWSDSIAPAISRGRRVLVAAHGNSLRALIKYLDNVSDDDIVGLNIPTGQPLVYELDD
                     QLTPIRHYYLGDPEAVARATAAVAAQGRAAPRG"
     gene            2806..4113
                     /locus_tag="AC16HB_03645"
     CDS             2806..4113
                     /locus_tag="AC16HB_03645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR5605913.1"
                     /translation="MTAKLLLAACLAIALGVPMIAADAAAQAKKSAAKKETKKKVAPR
                     RAEGAADVARKDQLQAEQRGLQQRLAQLKRQLAASEASHSEATDALRASEAAISTANR
                     RLRELASARRELERRIAEIQDRSRAIVSRQGEQERQFGLILRSQYAIARTSPWHALLD
                     GENPGQIGRDLQYLDYIARAKAQLIGELRARREELAGLETESRMRQTELAAIADEERA
                     SRAQLLQQQTAHKLTLDRLARQISSQRQSIATMERDERRLSTLIEQLTQALAEQTRRR
                     ERAPRATAPAAPRTADVEPPANSTFAQLRGRLNLPVQGEIAARFGSSRRTEAGVNAPT
                     WKGVFIRAPAGTEVHAIAAGRVIFADWLRGFGNLLILDHGEGFISVYGNNATLVPSVG
                     ETVGRGDVIAEVGSTGGSAEAGLYFELRYQGRPMDPLRWAAAR"
     gene            4167..5609
                     /locus_tag="AC16HB_03650"
     CDS             4167..5609
                     /locus_tag="AC16HB_03650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5605914.1"
                     /translation="MSGRMRNIGLIAAGAFAGVAISLGISAYAFRDSRGPIPLDDIRQ
                     FTDVFGVIKANYVEPIDDKKLMQEAISGMLAGLDPHSAFLDGDAFKELQTSTQGEFGG
                     LGIEVAPEDGAVRVVTPIDDTPAARAGIKSGDLIIKIDDKLTRGMQLNEAVKLMRGKK
                     GTAVTLTVLRKNEAQPLEFKLVRDVIRTQSVRAKMIEPGYGFIRISSFQERTVDDLVA
                     KINELYKQGPVKGLVLDLRNDPGGLLHAAIGVSAAFLPARTLVVSTDGRTEDARRRFV
                     AAPEDYQRGRGEDVLGKLPREVKTVPLVVLVNNASASASEIVAGALQDHKRAMLIGAQ
                     TFGKGSVQTIIPIRTDGDQPAAIKLTTARYFTPSGRSIQAKGIVPDMNVDDTPEGNFA
                     GLTVREADLARHLEDKSKPGDASATPPGASAAPAAPHATTPPSPAAQRRYEFGSTDDF
                     QLRQALNHLQGKPVVTAKPPENVAATAVTK"
     gene            5677..6429
                     /locus_tag="AC16HB_03655"
     CDS             5677..6429
                     /locus_tag="AC16HB_03655"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR5605915.1"
                     /translation="MNDQELLRYSRHILLNEIGVEGQQRLRDSHMLVIGAGGLGSPAA
                     FYLGSAGVGAITLVDGDNVDLTNLQRQILHTTERIGQPKATSGQATLAAINPQVEVIA
                     RTQRVAEADLDELVRGATVVLDCSDNFATRHAVNRACVRHGRPLVSGAAIRFDGQLAV
                     FDLTRADSPCYACLFPEGDGEDELCAVMGVFAPVTGIIGTLQAAEALKLAAKAGESLS
                     GRLVLLDALTMTFRTLNVPRDPDCSVCRARTT"
     gene            complement(6422..7030)
                     /gene="slmA"
                     /locus_tag="AC16HB_03660"
     CDS             complement(6422..7030)
                     /gene="slmA"
                     /locus_tag="AC16HB_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR5605916.1"
                     /translation="MDAASLSVESPSKRKPGARRLQILQTLATMLEDPKGEKVTTAAL
                     AAKLDVSEAALYRHFASKAQMFEGLIEFIESTIFGLINEISAKETSGLKQVRRIVMML
                     LEFAQTNRGMTRVLIGDALVNEHERLQERMNQLLDRIEASIRQSYRVAVTQNELPTEF
                     DPATHANLIIAYVVGRWLRFAKTGFKRLPAEGLDAQLPSLLT"
     gene            complement(7166..9013)
                     /gene="sppA"
                     /locus_tag="AC16HB_03665"
     CDS             complement(7166..9013)
                     /gene="sppA"
                     /locus_tag="AC16HB_03665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR5605917.1"
                     /translation="MPVGRPGLIRRFFGFLWRTLDFSRRLVLNLLFLAIVVVFVFAWF
                     YEARPTLADNTVLVLDLKGPIVEQYTGAASEAVLAQALLERQRETQLRDVLTALDAAA
                     KDSHIARVLLLLDDFEGAGQPTLREVAAALGRVRAAGKQIVAWGASFDQRQYFIAAHA
                     DEVYLHPQGMILMRGFGGYRNYYRDALDRLGISVNVFKAGKYKSFAEPFTQNAPSSEA
                     QQDEQQLLDDLWHQYTAAVETARKLPAGSIGRMIDELPQRFAAARGDAAQLALKEKLV
                     DGLKTRDELRSLLIERGAVDRERKTFRQIAFGDYLSLQPQPARGDAIGVVVAAGDIVD
                     GEAPQGMIGGRSTAELVRRARESERVKAVVLRVDSPGGSAFGSELIRRELELTQQAGK
                     PVIVSFGDVAASGGYWISMAADEVIADPTTVTGSIGVFALLPSADKAMEKLSLHTAGV
                     TTTWLGGAGDPRRPLDPRVGELIQTGVAHSYDEFIRLAASARKSTPEQINEIAQGRVW
                     TGRQAQQQQLIDRLGSYVDALKAAAVRAKLGDDFRVVYIEREPRGVDRLLALLFGETA
                     RVLQAGSGLNPEELIGLEGARQARRDLAWLRKAAANPFGAVAHCLCEPR"
     gene            complement(9062..9718)
                     /locus_tag="AC16HB_03670"
     CDS             complement(9062..9718)
                     /locus_tag="AC16HB_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR5605918.1"
                     /translation="MKRVWFFDLDGTLHDASHAIFAAIDTRMTEYVVRHLRVGHEQAN
                     HLRLTYWRRYGATMLGLIRHHAIDPHHFLRETHDFDVASLPRAERGLALLFGRLRGRK
                     VLLTNAPQAYAESVLTGIDLARRIGTRYSIELMRVHGTFRPKPSRAMLRHMLARERVA
                     RGNAILVEDSAANLKAARAVGFRTVLIGRGGRAGRASYVGLRVRSVRALPRALARLRG
                     "
     gene            complement(9715..10602)
                     /gene="argB"
                     /locus_tag="AC16HB_03675"
     CDS             complement(9715..10602)
                     /gene="argB"
                     /locus_tag="AC16HB_03675"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR5605919.1"
                     /translation="MDRAHVTPQMKADILSEALPYIRRFHGKTIVVKYGGNAMVDQNL
                     KEAFAHDVVLLKLVGMKPVVVHGGGPQIDEALERVGKKSTFIQGMRVTDAETMEIVEW
                     VLAGQVQGELVMLINRFGGQAVGMTGKDGSLIRARKLRMPDKDDPSKSHDIGHVGEVE
                     AINPAVVKALQDDAFIPVISPIGFGEDGTTYNINADVVAGKIAEILKAEKLVMLTNTP
                     GVLDRNGKLLTGLTAREIDDLFADGTISGGMLPKISSALDAARSGVHAVHIIDGRVDH
                     CLLLEILTDAGVGTMIRSH"
     gene            10701..10907
                     /locus_tag="AC16HB_03680"
     CDS             10701..10907
                     /locus_tag="AC16HB_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR5605920.1"
                     /translation="MQQSFQITGMHCNGCVNRVTKALQPLADEVTVTLDPPRAVLEAP
                     SAVSLDAVRAALARIGGYQASPLD"
     gene            complement(10904..11050)
                     /locus_tag="AC16HB_03685"
     CDS             complement(10904..11050)
                     /locus_tag="AC16HB_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605921.1"
                     /translation="MPRKRAGALAPRNPIALAARKRAAGPHGPTRKAQRLQEKRRLKK
                     LLEG"
     gene            complement(11050..11502)
                     /locus_tag="AC16HB_03690"
     CDS             complement(11050..11502)
                     /locus_tag="AC16HB_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR5605922.1"
                     /translation="MLRRLFVLVATLIALPALAQVSVSGAWARPTVQGQMGTGAFMTL
                     TAKDGARVVGAASPVAGVVEIHEMKMEGNVMKMRAVDALELPAGKAVELKPGGYHVML
                     LDLKQALKPGETIPLSLRIETHDRKLVTLPVEVEVARSAPPASGSHKH"
     gene            complement(11520..12842)
                     /gene="hslU"
                     /locus_tag="AC16HB_03695"
     CDS             complement(11520..12842)
                     /gene="hslU"
                     /locus_tag="AC16HB_03695"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR5605923.1"
                     /translation="MTPKEIVHELNKHIVGQDNAKRAVAIALRNRWRRQHVAEPLRSE
                     ITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDSIVRDLVD
                     ISIKQAREAEMKKVRAKAQDTAEDRILDVLVPPARDFGFSDARRDDASETRQKFRKRL
                     REGELDEKEIEIELAAPGAQMEVMAPPGVEELTQQIQAMFQNLGRERRKPRKLKISDA
                     LKLLIDEEAARLVNDEDLKTRALANVEQNGIVFIDEIDKIAARSEVQGADVSRQGVQR
                     DLLPLVEGTTINTKYGMVKTDHVLFIASGAFHYAKPSDLIPELQGRFPIRVELDSLTA
                     QDFERILTATDACLTEQYKALLATEEVKVDFTADGITHLAQIAYSVNEKTENIGARRL
                     YTVMERLLEEVSFDAGSRHGETLTVDAAYVEARLANLAGNEDLARYVL"
     gene            complement(12907..13443)
                     /gene="hslV"
                     /locus_tag="AC16HB_03700"
     CDS             complement(12907..13443)
                     /gene="hslV"
                     /locus_tag="AC16HB_03700"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR5605924.1"
                     /translation="MEPFHGTTIVSARRGAAVAMGGDGQVTLGNIVVKQSARKVRRLY
                     HDRILAGFAGATADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRILRRLEAMLA
                     VADRETSLIISGNGDVLEPEFGLVSIGSGGAYAQAAARALLDNTDLAPVDIVKKSLTI
                     AGDLCIYTNQSHTIESLD"
     gene            complement(13488..14966)
                     /locus_tag="AC16HB_03705"
     CDS             complement(13488..14966)
                     /locus_tag="AC16HB_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5605925.1"
                     /translation="MVFSFFKKDPKDPKQRPARPGTPARADVPARAGTPAPRPAAPAV
                     RATPLDGAPPEREVARSLAIETAAKIDAIESEMARDFLRPSGRNSDGPLTAAPQPMTG
                     NPAVVEAEPALKLVPKAEAKREVEEDDDFDPGSDLLAGSVDAIEINTSGAGSIIDETA
                     ILFSNGQIDAAEAVLRAGIRADDLAHATHNAWLMLFELINQRGDKKAFEQLTMEYVLR
                     FENTPSGWFDYVDAPVREPRVAAAVVTPASAGAEPAVRLAATVDASVVKQLEELKNLA
                     TSHAALRLDFSAVRSIDLVGAELLLRVINAFKRARHELTLAGAEQFLGPLRSATEPGR
                     RDASDALWMLMLEILRVLDRQEEFDETAIQYCITFEVSPPSWEPAPPNIKPGPATALE
                     PMPQIDAAVDFFEWRGAIDGEGEPHFGRLLIAAPANPQLIVECAQLRRMAFSAASAML
                     TLSRRVQKAGSSLELRNVNPMIAALLHLLGVDGIATVQPRRN"
     gene            complement(15241..>15492)
                     /locus_tag="AC16HB_03710"
     CDS             complement(15241..>15492)
                     /locus_tag="AC16HB_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605926.1"
                     /translation="AARSGGRAAADASPRDARRRRSLPSPAQHRWRGARELPEFMPQI
                     VALERRGVKLRGFMMPIVAGETHEHFLLTAAQLSSAPHP"
BASE COUNT         2493 a         5335 c         5119 g         2545 t
ORIGIN      
        1 actccggcca gccccaggaa cttctcggcg cgcgcgccga gcgcggcgcc cagccgcgcg
       61 atctcggcca ggccgcgcgt gatcagcgcc gcgcgcgcgt tgtggccgag gccgagcgcg
      121 tcgctgatgc cggtggcgat cgccatcacg ttcttcactg caccgccgac ctcgaccccg
      181 atcacgtcgt cgctcgagta gatccgcaat gcgccgccgt ggaagatctg cgtgacccag
      241 cggcagaact gcgcgggtcc ggcgacggtc agcgcggtcg gcagaccgcg tgcgacctcg
      301 gcggcaaagc tcggccctga cagcggaccg ctcggatgcg acgcgatctc gtcgcgcacg
      361 atctcgtgcg ccagcttggc gctgtcgcgc tcgaagccct tgcacaacca cacgagcggt
      421 gccaacgccg tctcggcgtg caggccgcgc acggtctcgc gcagcgccgc cgtgggcacg
      481 gcgagcacga gcagcccgtg ccgcgcgtgc gcgaccgccg cggccaagtc cggcgtcacc
      541 gtgatcgatg cgtcgagcac cgtgcccgcc agataacggt cattgcggcg cgtggccgcg
      601 agcgcacgca cttgcgccgc gtcatgcgac cacagcgtca ggtgatgacg ctgcgcgagc
      661 acgctggtga tcgcggtgcc ccaggcgccc gcgccgagga ccgcgacctg cgccatgcgc
      721 gtgcgtccgg cttactgcgt ggtcgccggc gcggcggtcg cgattccctg ctgctgctgc
      781 gcaatgcgct gctggtacat cgcctcgaag ttgatctccg ccaggtgcac cgaaggcagc
      841 ccggtgcgat tgatcaggtc ggcgacgttc gcgcgcagat acggatagat gatgccgggg
      901 cagacgatgc cgaggatggc ctcgaactgc tcgttcggga tgccgcgcat ttcgaagatc
      961 ccggcctgct tgccctcgac caggaacagc accttgtcgc cgaccttggc ggtggtcgtg
     1021 gcgcgcacca ccacctcgta gatgccgtcg acgaccttgc tgttcgacac ctcgagctgc
     1081 acgtcgacct gcggcgcggt ctgttcgagg aagatctgcg gcgcgtgcgg cagttcgagc
     1141 gaggcatcct tcagatagat gcgttgcagc tgaaacaccg cctgcggcgc agtctgcggg
     1201 ctgacgatct cggccatttc gatcctttct tgaggtgccg gctgcctgcc ggcggtgcga
     1261 tttcggggcg gggatccgga aaggctaggc ggcgagcagc ggctgcagtt tgcccgcgcg
     1321 atcgagcgcg gacaggtcgt cgaagccgcc gacgtgcgtc tccccgatgt agatctgcgg
     1381 cacggtgcgg cgtccggtac gggcaatcat ttcgtcacgc cgcgcggcgt ccaggtcgat
     1441 gcggatcttt tcgatctcgg tcacgccacg cgccttcagc aacgcttcgg cacgcacgca
     1501 gaacggacac accgcggtgc tgtacatcct gacgcgggca gtcatccggc tcggtctttc
     1561 gcggtcatgc ctttaccggc agtccggcct cgcgccaggc agccagcccg ccggcgagca
     1621 cgtacacgtc ggtgaagccg gcggcgcgca gttgcgccgc cgcgggtccg gcgctgcgcc
     1681 cggtgttgca cacgaggatc accgggcggt ctttcttcag ctcgccggtg cgcgatgcga
     1741 tccgcgcggt cgggatgttc ttcgcgttcg gcagcgaacc cttgccgaag tcgtcctcgc
     1801 tgcgcacgtc gacgacctgc gcatgcctgg agttgatgag ctgcgtggcc tgcagcgtcg
     1861 ataccgacgg accggccgcg cgcgcgcgaa tcaacggcca gagcagcatg ccgccgctga
     1921 tgaacgccac cgcgatcagg aagatgttgt cgatgaagaa acgcacgggc aacggcgaga
     1981 tcggacgaag gactcgaatt atagaatgcg ggtgtcgatc cgacctgtgc cgtgactccg
     2041 atgcaaaagc tcgttctatt gcgccatggc gaaagccagt ggaatctcga aaaccgcttc
     2101 accggctgga ccgacgtcga tctatcggac aacggcatcc gcgaggcgcg cgcggcgggc
     2161 gaactgctgc agcgcgaggg ctacgtgttc gacatcgcgt acacgtcggt cctcaagcgc
     2221 gcgatccgca cgctgtggat caccctcgat gcgctcgact tgatgtggct gccggtggtc
     2281 cactcgtggc gtctcaacga gcgccactat ggcgcgctgc aggggctgaa caaggccgaa
     2341 accgcagcga agtacggcga acagcaagtg ctgacctggc gccgcgcgta cgcgatcgct
     2401 cccgagccgc tcgcgcgcga cgatccgcgc tgggcgggaa atgatccgcg ctaccggcat
     2461 ctgaagcccg aggacatccc ggccaccgaa tgcctgaagg acacggtcgc gcgcgtggtg
     2521 ccgttctgga gcgattcgat cgcgccggcc atctcgcgcg gccgccgcgt gctggtggcc
     2581 gcgcatggca actcgttgcg cgcgttgatc aagtatctcg acaacgtgtc cgacgacgac
     2641 atcgtcggcc tgaacatccc gaccgggcag ccgctggtgt acgagctgga tgatcaattg
     2701 acgccgatcc gccattacta cctcggcgat cccgaggcgg tcgcgcgcgc cacggcggct
     2761 gtggcggcgc agggacgggc tgcgccgcgc gggtagccgg acgcattgac agcgaagctc
     2821 ctcctcgcag cgtgcctggc gatcgcgctc ggcgtgccga tgatcgcggc cgacgccgcg
     2881 gcccaggcaa agaagtccgc agcgaagaag gagacgaaga agaaggtcgc gccgcggcgc
     2941 gccgagggtg cggcagatgt ggcgcgcaag gaccagctgc aggctgagca gcgcggcctg
     3001 cagcagcgcc tcgcgcagct gaagaggcag cttgccgcct cggaggcgtc gcactcggag
     3061 gccaccgatg cgctgcgcgc gtccgaggcg gcgatctcga ccgccaaccg ccgcctgcgc
     3121 gaactggcgt ccgcgcgcag ggaactggaa cgcaggatcg ccgaaatcca ggaccgcagc
     3181 cgtgcgatcg tctcgcgcca gggcgagcag gagcggcagt tcggcctcat cctgcgcagt
     3241 cagtacgcga ttgcgcgcac gtcgccatgg cacgcgttgc tcgacggcga gaatccggga
     3301 cagatcggcc gcgatctgca gtacctcgac tacatcgccc gcgcgaaagc gcaactgatc
     3361 ggagaactgc gggcgcgccg ggaagagctg gccggactgg agaccgaatc gcggatgcgc
     3421 cagaccgaac tggccgcgat cgccgatgag gaacgggcca gtcgcgcgca actgctgcag
     3481 cagcagaccg cgcacaagct gacgctcgac cgcctggccc ggcagatcag ttcgcagcgg
     3541 cagtcgatcg caacgatgga gcgcgacgag cgccggctgt cgacgctcat cgagcagttg
     3601 acgcaggccc tcgcggagca aacacggcgg cgcgaacgcg cgccgcgcgc caccgctccc
     3661 gcggcaccgc gcacggcgga cgtggagccg cccgccaact ccactttcgc gcaactgcgc
     3721 gggcggctga atctgccggt gcagggggag atcgcggcgc gcttcggcag ttcgcgccgc
     3781 accgaagccg gcgtcaatgc gccgacctgg aagggggtct tcatccgcgc acccgccggc
     3841 accgaggtgc acgccatcgc cgccggtcgg gtcatcttcg ccgactggct tcgcggcttc
     3901 ggcaacctgt tgattctcga ccacggcgaa ggcttcatct cggtctatgg gaacaacgcc
     3961 acactggtcc ccagcgtggg cgagacggtc gggcgcggcg acgtgatcgc agaggtggga
     4021 agcaccgggg gcagcgcaga ggccggccta tacttcgaac tgcgctacca gggtcgaccc
     4081 atggacccgt tgcggtgggc agcggcgcgc taagctgttg gaactttcgt cctgccccgc
     4141 gatctgttgg aactccggga ggtgacatga gcggacgcat gcgcaacatc ggactgatcg
     4201 ccgccggcgc cttcgccggc gtcgcgatca gtctcggaat ttctgcctac gccttccgcg
     4261 attcgcgcgg cccgatcccg ctcgacgaca tccgtcagtt caccgacgta ttcggcgtga
     4321 tcaaggccaa ctacgtcgag ccgatcgacg acaagaagct gatgcaggaa gcgatctccg
     4381 gcatgctggc cggcctcgac ccgcattcgg cgttcctcga cggcgatgca ttcaaggaac
     4441 tgcagaccag cacgcagggc gagttcggtg gcctcggcat cgaagtggcg ccggaggacg
     4501 gcgcggtgcg cgtggtgacg ccgatcgacg acacgcccgc tgcgcgcgcc ggcatcaagt
     4561 cgggcgacct gatcatcaag atcgacgaca agctgacgcg cggcatgcag ctgaacgagg
     4621 cagtcaagct gatgcgcggc aagaaaggca cggcggtcac gctgaccgtg ctgcgcaaga
     4681 acgaggcgca gccgctcgaa ttcaagctgg tgcgcgatgt cattcgcacg cagtcggtgc
     4741 gcgccaagat gatcgagccg ggctacggct tcatccgcat ctcgagcttc caggagcgca
     4801 ccgtcgatga tctggtggcc aaaatcaatg agttgtacaa gcagggcccg gtcaagggcc
     4861 tcgtgctcga cctgcgcaat gacccgggcg gcctgctgca cgcggccatt ggcgtctccg
     4921 ccgctttcct tccggcacgt acgctggtgg tctccacgga cggccgcacc gaggacgcgc
     4981 ggcgacgctt cgttgccgcg cccgaggatt accagcgcgg ccgcggcgag gacgtgctcg
     5041 gcaagctgcc gcgcgaggtc aagacagtgc cgctggtggt gctggtcaac aacgccagcg
     5101 catcggcatc cgagatcgtc gccggcgcgc tgcaggacca caagcgcgcg atgctgatcg
     5161 gcgcgcagac cttcggcaag ggctcggtgc agacgatcat cccgatccgc accgacggcg
     5221 accagccggc cgcgatcaag ctcaccaccg cgcgctactt cacgccgagc ggacgctcga
     5281 tccaggccaa gggaatcgtg cccgacatga atgtcgacga cacaccggaa ggcaattttg
     5341 ccggcctgac cgtgcgcgaa gcagatctgg cacgccatct ggaggacaag tccaaaccgg
     5401 gcgatgcgag cgcaacgccg ccgggcgcct cggccgctcc cgccgcgccg cacgcgacga
     5461 cgccaccgtc ccccgccgcg cagcgccgct acgagttcgg ttcgaccgac gacttccagt
     5521 tgcggcaggc gctgaaccac ttgcagggca agccggtcgt caccgcgaag ccgccggaga
     5581 acgtggccgc gacggcggtc accaaataga cacgccggat gacgactgca ggggccgcgc
     5641 agcggccctt gtcatttgca gcggccggac agaccgatga acgaccagga actgctgcgc
     5701 tattcgcgcc acatcctgct gaacgagatc ggcgtcgagg ggcagcagcg cctgcgggat
     5761 tcgcacatgc tggtgatcgg cgcgggcggc cttggctcgc ccgccgcctt ctacctgggg
     5821 tcggccggcg tgggcgccat cacgctggtc gacggcgaca acgtcgatct gacgaacctg
     5881 caacggcaga tcctgcacac gaccgagcgg atcggccagc cgaaggcgac ctcgggtcag
     5941 gcaacgctgg ccgcgatcaa tccacaggtc gaggtgatcg cgcgcacgca gcgcgtcgcc
     6001 gaggcggacc tcgacgaact ggtgcgcggc gcgaccgtcg ttctcgactg ctcggacaac
     6061 tttgctaccc ggcatgcggt caaccgggcg tgcgttcgtc acggccggcc gctcgtgtcc
     6121 ggcgcggcga tccgcttcga cggtcagctc gcggtgttcg acctgacgcg cgccgatagc
     6181 ccgtgctacg cgtgcctgtt cccggagggc gacggtgagg acgaactgtg cgcggtgatg
     6241 ggcgtgttcg cacccgtgac gggcatcatc ggcacgctgc aggcggccga ggcgctgaag
     6301 ctggccgcga aagcaggcga atcgctgtcg ggacggctgg tgctgctcga cgcgctgacg
     6361 atgactttcc gcacgctcaa cgtgccgcgc gatccggact gctcggtgtg tcgcgcgcga
     6421 actacgtaag cagcgacggc agctgcgcgt cgagaccctc ggccggcaac cgcttgaagc
     6481 cggtcttggc aaagcgcagc cagcgcccga ccacgtaggc aatgatcagg ttcgcgtggg
     6541 tggccgggtc aaactcggtc ggtagttcgt tctgcgtgac cgcgacgcgg taactctggc
     6601 gaatcgaggc ctcgatccgg tcgagcagct ggttcatccg ctcctgcagc cgttcgtgtt
     6661 cgttgaccag cgcatcgccg atcagcacgc gcgtcatgcc gcggttggtc tgagcgaatt
     6721 cgagcagcat catgacgatc cgccgcacct gcttcagacc cgaggtctct tttgcggaga
     6781 tctcgttgat cagtccgaag atggtcgact cgatgaactc gatcagaccc tcgaacatct
     6841 gcgccttgct cgcgaagtgc cggtacagcg ccgcttccga cacatcgagc ttggccgcca
     6901 gggctgccgt ggtgaccttt tcgcccttcg ggtcttcgag catcgtcgcc agcgtctgca
     6961 ggatctgcag cctgcgcgcg ccgggcttgc gcttactggg tgattcgacc gacagacttg
     7021 cggcgtccat tgccacctcg catcagtggc caaaatctac cgtaatgccg cgtgacgtgg
     7081 ggaattactt ccatcgggaa gccggccgat acacggattt gctaacccgc gtggactaaa
     7141 tcgaacggcg gggcaggaag ctgactcagc gcggttcgca caggcagtgc gcaacggcgc
     7201 caaacggatt ggcagccgcc ttccgcagcc atgcaaggtc gcgccgcgcc tgtcttgcgc
     7261 cttcgagtcc aatcagttcc tccgggttca gccccgatcc tgcctgcagc acgcgggcgg
     7321 tctcgccgaa cagcagcgcc agcagccgat cgaccccgcg cggctcgcgt tcgatgtaga
     7381 cgacacggaa gtcgtcgccc agcttcgcgc gcacggcggc ggccttcaac gcgtcgacat
     7441 agctcccgag ccggtcgatc agctgctgct gctgtgcctg ccgaccggtc catacgcgcc
     7501 cctgggcgat ttcattgatc tgctccggcg tcgacttccg cgcagaagct gccagtcgga
     7561 tgaattcgtc gtagctatgg gccaccccgg tctggatcag ctcgccgaca cgcggatcga
     7621 gcgggcggcg cggatcgccg gcgccgccga gccacgttgt ggtgacgccg gccgtatgca
     7681 gcgacagctt ctccatcgcc ttgtcggcgg agggcagcag cgcgaacacg ccgatcgaac
     7741 cggtcaccgt ggtgggatcc gcgatcacct cgtcggccgc catcgagatc cagtatccgc
     7801 ccgacgcggc aacatcgccg aacgacacga tcaccggctt gccggcctgc tgcgtcagct
     7861 cgagttcgcg ccggatcagc tccgacccga acgcgctgcc gcccggcgag tcgacccgaa
     7921 gcacgaccgc tttcacgcgc tcgctttcgc gcgcgcgccg caccagttcc gcggtcgagc
     7981 gcccgccgat catcccctgc ggcgcttcgc cgtcgacgat gtcgcccgcg gcgaccacga
     8041 cgccgatcgc gtcgccccgc gcaggctgtg gctgcaacga caggtagtcg ccgaacgcga
     8101 tctggcgaaa cgtcttgcgc tcgcgatcga ctgcgccgcg ttcgatgagc aggctgcgca
     8161 gttcgtcgcg cgttttgagt ccgtcgacca gcttctcctt cagtgcaagc tgtgccgcat
     8221 cgcccctcgc cgcggcgaaa cgctgcggca gttcatcgat cattcgcccg atgctgccgg
     8281 ccggcagctt gcgcgccgtc tcgaccgcgg ccgtgtactg gtgccacaga tcatcgagca
     8341 gttgctgctc gtcctgctgc gcttccgacg acggcgcgtt ctgcgtaaac ggctcggcaa
     8401 agctcttgta tttgccggcc ttgaacacgt tgaccgagat gcccagccgg tcgagcgcgt
     8461 cgcggtagta attgcggtag ccgccgaagc cgcgcatcag aatcatgccc tgcgggtgca
     8521 gatacacctc gtccgcgtgc gcggcaatga aatactggcg ctggtcgaac gacgcgcccc
     8581 aggcgacgat ctgcttgccg gcggcccgca cgcgtccgag tgcggcggcg acctcgcgca
     8641 gcgtcggttg gcccgcgccc tcgaaatcgt ccagcagcag gagcacgcgc gcgatgtgcg
     8701 aatccttggc cgcagcatcg agcgcggtca gcacgtcgcg caactgcgtc tcccgctggc
     8761 gctcgagcag tgcctgcgcc agcaccgcct cgctcgcggc gcccgtgtac tgctcgacga
     8821 tcggaccctt gaggtcgagc accagcacgg tgttgtcggc cagcgtcggc cgggcttcat
     8881 agaaccacgc gaagacgaat acgacgacga tcgcgaggaa cagcaggttg agcaccagcc
     8941 ggcgcgagaa atcgagcgtc cgccagagga aaccgaaaaa gcgtcgaatc aggccagggc
     9001 ggccgacagg catgggcaac tccgaatgca gtccgatcgc ccgaaagctt acagcgcgcg
     9061 tctaaccacg cagccgggcc aacgcgcgcg gcagagcccg caccgagcgc acgcgcagtc
     9121 cgacatagct cgcgcgcccc gcgcgcccgc cgcgaccgat gagcacggtg cgaaagccga
     9181 ccgcgcgcgc cgctttcagg ttggcggcac tgtcctcgac cagaatcgcg tttccgcgcg
     9241 cgacgcgttc gcgcgccagc atgtggcgca gcatcgcacg cgagggtttg gggcggaacg
     9301 tgccgtgcac gcgcatcaat tcaatcgaat accgcgtgcc gatgcgccgc gcgaggtcga
     9361 tgccggtcag caccgactcc gcatacgcct gcggcgcatt ggtaagcagc accttgcgcc
     9421 cgcgcagccg gccgaacagc agcgcgagcc cgcgctcggc gcgcggcagc gatgcaacgt
     9481 cgaaatcgtg ggtctcgcgc aggaaatggt gcgggtcgat cgcgtgatgc cggatcagcc
     9541 cgagcatcgt cgcgccgtag cgccgccagt aggtcaaccg caggtggttc gcctgctcgt
     9601 gcccgacccg aaggtgccgc acgacgtact ccgtcatccg cgtgtcgatc gccgcgaaga
     9661 tcgcgtgcga cgcgtcgtgc agcgtgccgt cgaggtcgaa gaaccagacg cgcttcaatg
     9721 gctgcgaatc atggtgccga cgccggcatc ggtcagaatc tcgagcagca ggcagtggtc
     9781 gacgcggccg tcgatgatgt gtaccgcgtg gaccccgctc cgcgccgcgt cgagcgcgga
     9841 cgagatcttc ggcagcatgc cgccggagat cgtgccgtcg gcgaacagat cgtcgatctc
     9901 gcgcgccgtc aatcccgtca gcagcttgcc gttgcgatcg aggacgccgg gcgtgttggt
     9961 cagcatcacc agcttctcgg ccttgaggat ctcggcgatc ttgccggcca ccacgtcggc
    10021 gttgatgttg taggtggtgc cgtcctcgcc aaagccgatc ggcgagatca ccgggatgaa
    10081 tgcgtcgtcc tgcaaggcct tgacgaccgc cggattgatc gcctcgacct cgccgacatg
    10141 gccgatgtcg tgcgacttgc tgggatcgtc cttgtcgggc atgcgcagtt tgcgcgcgcg
    10201 gatcaggctg ccgtccttgc ccgtcatgcc gaccgcctgg ccgccgaagc ggttgatcag
    10261 catgaccaac tcgccctgca cctggccagc cagcacccac tcgacgatct ccatcgtctc
    10321 ggcgtcggtg acacgcatgc cctggatgaa ggtcgacttc ttgccgacgc gttcgagcgc
    10381 ttcgtcgatt tgcggaccgc cgccgtgcac gacgaccggc ttcatgccga ccagcttcag
    10441 cagcaccacg tcatgggcga aagcttcctt cagattctgg tcgaccatcg cgttgccacc
    10501 atacttgacc acgatggtct tgccgtggaa gcggcggatg tacggcagcg cttcggacag
    10561 gatgtccgcc ttcatctgcg gcgtgacatg ggcgcgatcc atcgcgggaa ttcagagttt
    10621 tgtggcgcgg cgcagtttat cagcggtcga tgcgcctgga cggagcagaa tacgcagcgt
    10681 cgaagcgcag gagccgaacc atgcaacaga gcttccagat caccggcatg cactgcaacg
    10741 gctgcgtaaa ccgcgtcacc aaggcgctgc agccgctggc ggacgaggtc accgtcacgc
    10801 tcgatccgcc gcgcgccgta ctcgaagccc cgagcgcggt atcgctcgat gccgtgcgcg
    10861 ccgcgctggc tcggatcggc ggctaccagg cgagtccgct cgactagcct tcgagcagct
    10921 tcttcagcct tcgcttctcc tgcagccgct gcgccttgcg cgtcgggccg tgcggcccgg
    10981 ccgcgcgctt gcgcgcggcc aatgcgatcg gattgcgcgg tgcaagtgca cccgcgcgct
    11041 tgcggggcat cagtgcttgt ggctgccgct cgccggcggc gcgctccggg cgacctcgac
    11101 ctcgaccggc aatgtgacca gcttgcggtc atgggtctcg atgcgcaacg acagcgggat
    11161 cgtttcgccc ggtttcagcg cctgtttgag gtcgagcagc atcacgtgat agccgccggg
    11221 cttcagttcc actgccttgc cggccggaag ttcgagcgca tcgaccgcgc gcatcttcat
    11281 cacgttgccc tccatcttca tctcgtggat ctcgaccacg cccgcgacgg gacttgccgc
    11341 accgacgacg cgcgcgccgt ccttcgcggt cagcgtcatg aaggcgccgg tgcccatctg
    11401 cccctgcacc gtcggtcgcg cccacgcgcc gctcacgctg acctgtgcga gcgcgggcag
    11461 cgcaatcagc gtggcaacca gaacgaacag tcggcgcaac atccggttcc cctctctcgt
    11521 caaagtacgt agcgtgccag atcctcgttg ccggcgagat tcgccagccg cgcctcgaca
    11581 taggcggcgt cgaccgtcag cgtttcgccg tgacggctgc cggcgtcgaa cgacacttct
    11641 tcaagcagcc gctccatgac ggtgtacagg cggcgcgcgc cgatgttctc ggtcttctcg
    11701 ttgaccgagt acgcgatctg cgccaggtgc gtgatcccgt cagcggtgaa atcgaccttg
    11761 acttcctcgg tcgccagcag cgccttgtac tgctcggtca ggcaagcgtc ggtcgcggtc
    11821 aggatgcgct cgaaatcctg cgcggtcagc gaatcgagtt ccacccggat cgggaatcgc
    11881 ccctgcagtt ccgggatcag gtcactcggc ttggcgtagt gaaacgcgcc cgaggcgatg
    11941 aacaggacgt ggtcggtttt caccatcccg tacttggtgt tgatcgtcgt gccttcgacc
    12001 agcggcagca ggtcgcgctg cacgccctgg cgcgacacgt cggcgccctg gacttccgag
    12061 cgcgcggcga tcttgtcgat ctcgtcgatg aacacgatgc cgttctgctc gacattggcg
    12121 agcgcgcgcg tcttcaggtc ctcgtcgttg accagccgcg cggcctcctc gtcgatcagc
    12181 agcttcagcg cgtcgctgat cttcagcttg cgcggcttgc gccgctcgcg gccgaggttc
    12241 tggaacatcg cctggatctg ctgcgtcagc tcctccactc cgggcggcgc catcacctcc
    12301 atctgcgcgc ccggcgccgc cagttcgatc tcgatctcct tctcgtcgag ttcaccctcg
    12361 cgcagcctct tgcggaactt ctgtcgcgtc tcgctcgcgt cgtcacgccg cgcatcggag
    12421 aagccgaagt cacgcgccgg cggcaccaac acgtcgagga tgcggtcctc ggccgtgtcc
    12481 tgcgccttgg cgcgcacctt cttcatctcc gcttcgcgcg cctgcttgat cgagatgtcc
    12541 accaggtcgc gcacgatcga gtcgacatcg cgccccacgt agccgacctc ggtgaacttg
    12601 gtggcctcca ccttgatgaa gggcgcgtcc gccagccgcg ccagccgccg cgcgatctcg
    12661 gtcttgccca cgcccgtggg cccgatcatc aggatgttct tcggcgtgat ctccgagcgc
    12721 agcggctcgg ccacgtgctg gcgccgccag cggttgcgca gcgcgatcgc gaccgcgcgc
    12781 ttggcgttgt cctggccgac gatgtgcttg ttcagctcgt ggacgatttc cttgggggtc
    12841 atcatggcag cagcggcccg gatgtgcgcg acgccgcgcg tcgcatctgc accctcaccg
    12901 atcaggtcag tccagcgact cgattgtgtg cgactggttg gtgtagatgc acaggtcacc
    12961 ggcgatggtg agcgacttct tgacgatatc gaccggcgcc agatccgtat tgtcgagcag
    13021 cgcgcgcgcc gccgcctgcg cgtaggcgcc gccggagccg atcgagacca gcccgaactc
    13081 gggctccagc acgtcgccgt tgccgctgat gatcagcgac gtttcgcgat cggcgaccgc
    13141 cagcatcgcc tccagccgtc gcaggatgcg gtcggtacgc cagtccttcg ccaactcgac
    13201 ggcggagcgc agcaggttgc cctggtgctt ctccagcttc gcctcgaagc gctcgaacag
    13261 cgtgaacgca tcggcggtcg cgccggcgaa cccggctagg atgcgatcgt gatacaggcg
    13321 ccgcaccttg cgcgcgctct gcttgacgac gatgttgccg agcgtgacct ggccatcacc
    13381 gcccatcgcg accgccgcgc cgcggcgtgc ggacacgatg gtggtgccgt gaaagggttc
    13441 catttagcgc gacatggggt cgcgtcgcgc gggattcaag gccgctttca gttgcggcgc
    13501 ggctgcaccg ttgcaatgcc gtcgacgccg agcagatgca gcagcgccgc gatcatcgga
    13561 ttgacgttgc gcagctcgag cgaactgccg gccttctgca cgcggcgtga aagcgtgagc
    13621 atcgcggagg ccgcggagaa cgccatgcgc cgcagttgcg cacactcgac gatcagttgc
    13681 gggttcgcag gcgccgcgat gagcagacgc ccgaaatgcg gttcgccctc gccgtcgatc
    13741 gcgccgcgcc actcgaagaa atcgaccgcg gcgtcgatct gaggcatcgg ctcgagcgca
    13801 gtcgcgggac ccggcttgat gttcggcggc gccggctccc acgacggcgg cgagacctcg
    13861 aacgtgatgc agtactggat cgccgtctcg tcgaattcct cctggcggtc cagcacgcgc
    13921 aggatctcga gcatcagcat ccacagcgcg tcggaggcgt cgcggcggcc gggttcggtc
    13981 gcgctgcgca acggcccgag gaactgttcg gctccggcca gcgtcaattc atggcgcgcg
    14041 cgcttgaacg cattgatcac ccgcagcaac aattcggcgc ccaccagatc gatcgatcgc
    14101 accgcggaaa agtcgagcct cagcgcggcg tgcgaggtcg cgagattctt cagctcctcc
    14161 aactgcttca ccacgctggc gtcgaccgtc gcggccagcc gcaccgccgg ctcggcgccc
    14221 gcagatgcgg gcgtcacgac cgctgcggcc acacgcggct cgcgcacggg cgcgtccacg
    14281 taatcgaacc atcccgacgg cgtgttctcg aaccgcagca cgtattccat cgtcagctgt
    14341 tcgaacgcct tcttgtcgcc acgctggttg atcagttcga acagcatcag ccaagcgttg
    14401 tgcgtggcgt gcgcgagatc gtcggcgcgg atgccggcgc gcagcaccgc ctcggctgcg
    14461 tcgatctggc cgttggaaaa caggatcgcc gtttcgtcga tgatcgagcc cgcgcccgag
    14521 gtgttgatct cgatcgcatc gacgctgcct gccagcaggt cggaacccgg gtcgaaatcg
    14581 tcgtcttcct ccacctcgcg cttggcctcc gctttcggca ccagtttcag tgccggctcg
    14641 gcttcgacga cggccgggtt gcccgtcatc ggctgcggcg cggcggtcaa cggtccgtcg
    14701 gaattgcgcc cggacggtcg caggaagtcg cgcgccatct cgctttcgat ggcgtcgatc
    14761 ttcgccgcgg tttctatcgc cagcgaacgg gccacctcac gctcgggcgg cgcgccgtcg
    14821 agcggcgtgg cgcgcacggc gggggcggca ggtcgcggcg ccggcgttcc cgcgcgggcc
    14881 ggcacatccg cgcgcgcagg tgtacctggc cgcgccggac gctgcttcgg gtccttgggg
    14941 tccttcttga agaacgagaa aaccacgtgt gctctgcaga atcctggcgc ggtcaaccgg
    15001 gccgactgca cggcccaccg tccctggcga tcgtcgtcct gtcgcctcgc ccagcaattt
    15061 ggaccgggag gctcgtgacc gacggagtcg cgggttattc gccgcccgtc gatcgctttg
    15121 ccgccccgcg tccatcagtc ggcaaatcac tataacaact ttcgctttcc gggctcatcg
    15181 tccgacgggc tgcccggacc gatcgactac cgtctaggcg ttaggtgccc ggcaccgcgg
    15241 ctacgggtgc ggggcgctcg aaagctgcgc cgctgtcagc aggaagtgct cgtgcgtctc
    15301 gccggcaacg atcggcatca tgaagccccg cagcttcacc ccgcgccttt cgagcgcgac
    15361 gatctgcggc atgaattccg gcagctcgcg cgcgccgcgc cagcggtgct gcgctgggga
    15421 cggtagagat cgtcgccgtc gagcatcacg cgggcttgca tcggcggcag cccgcccgcc
    15481 agaacgtgca gc
//
LOCUS       DBNHBY010000149         4695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1767494, whole genome shotgun sequence.
ACCESSION   DBNHBY010000149 DBNHBY010000000
VERSION     DBNHBY010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1767494"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16HB_03715"
     CDS             complement(<1..473)
                     /locus_tag="AC16HB_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5605927.1"
                     /translation="MLKEEHHLLRDSLRAFAQSRLAPFAAQWDRDHTFPKDALRELAA
                     LGAFGIAVPEQYGGAGMDYTALAVALEEIAAGDGGVSTIISVNNCPVCAILMGYADEA
                     QKQRFLKPLASGAALGAFCLTEPHVGSNAAALRTTARRDGDHYVLNGVKQFITSG"
     gene            complement(485..925)
                     /locus_tag="AC16HB_03720"
     CDS             complement(485..925)
                     /locus_tag="AC16HB_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605928.1"
                     /translation="MRHLSLPLIAALLFGICGGVAAASLRFTLREFAIESGVNGWVFV
                     AVPGLFAGLFALIVYQGAEQRVQRVGPSMTRGLLVALLTWFGFSALATAVWFPIEDFF
                     NWLSTVLLLTGFVGGGPMLLAALVAGALVGWVIVRRGRAWVLKT"
     gene            complement(987..1370)
                     /locus_tag="AC16HB_03725"
     CDS             complement(987..1370)
                     /locus_tag="AC16HB_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5605929.1"
                     /translation="MQRRLISSGSSFEAVAGYSRAVVDGDTVHVSGTTGFDYATMTIE
                     PDPVAQCRQCFRNIAAALGEADCTLDDVVRVHYYLTDAALFERLAPIFGEVFGRARPA
                     ATAIVCGLIDPRMAIEIEVTACRRR"
     gene            complement(1378..2055)
                     /locus_tag="AC16HB_03730"
     CDS             complement(1378..2055)
                     /locus_tag="AC16HB_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5605930.1"
                     /translation="MPTVLVIGASRGIGFEFARQYADDGDRVIATHRNPDDAGKLRAL
                     GAKTIELDVTDETGIAALAWHLDGDSVDLAILNAGVSIRPRTDGLVAPTPRDFDHVMH
                     TNVRAPMQLLPILAPSLLRAKGKLAVMSSRMGSSSLMTAAGSWLYRASKAALNNVLRA
                     ASIELGARGVVCMAFHPGWVRTDMGGPEGEVDVRDSVAGMRRVIAAASASHNGQFFDY
                     TGERLSW"
     gene            complement(2067..3248)
                     /locus_tag="AC16HB_03735"
     CDS             complement(2067..3248)
                     /locus_tag="AC16HB_03735"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR5605931.1"
                     /translation="MSESIVIVSAARTPIGGLLGDFSSQQAYQLGAVAIKAAVERANV
                     SRDAIDEVLFGCCLMAGQGQAPARQATLLAGLPASAGAVTLSKMCGSGMRTMMFAHDM
                     LLAGSANVLVTGGMESMTNAPHLLVGARKGYRYGAATMYDHMALDGLEDAYERGKAMG
                     VFAEQCVAKYEFTREAQDKFAIASTERALKANTDGSFAWEIAPVMIPGKGGEVKIDKD
                     EQPFKAKLDKIPGLKPAFKKDGTITAANASSISDGAAALVMMRQSTADKMGCKPIARV
                     LAHAVHAQAPEWFTTAPVGAIDKVLKKVGWKAEQVDLWEVNEAFAAVTMAAMKEHRIP
                     HEKVNIHGGACALGHPIGASGARIVVTLLGALKKTGGKRGVAALCIGGGEATALALEM
                     M"
     gene            3346..>4695
                     /locus_tag="AC16HB_03740"
     CDS             3346..>4695
                     /locus_tag="AC16HB_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5605932.1"
                     /translation="MADGPDFKLTYSTMFDPPPALHQRFEAALARVRGQLGQDYPLWI
                     GGRERLADTTFEVRSPIDRDWLIGCFQAGTARHADAALTAAREAFPAWAARPWQERVR
                     LLRRAAALIEERVYDIGAAVALEVGKNRMESLGEVQETADLIAWYCDQMEANAGFARE
                     LPNDPLAGLVSRNRTVLKPYGPWVVIAPFNFPFALAGGPIGAALVAGNTVVFKVASDT
                     SLSGWLLLEALRDAGLPDGVVNFVTGAGGEVGNALIAHGDVAGVTFTGSYEVGMGIVR
                     AFAQRPWPRPSIAEMGGKNAAIVSRHADIERAALGILRSAFGLQGQKCSACSRVYVER
                     PLADPLRERLVESARAIKVGDPTLRENWMGPVINAAALARYEKCAQQLKQHGAVFFGG
                     EMLKRDGLERGYFVAPTIGTAPLDYRLWREEKFIPLVLVAEVDSVDDALRLANQSEYG
                     "
BASE COUNT          711 a         1636 c         1610 g          738 t
ORIGIN      
        1 ttgccgctgg tgatgaattg cttgacgccg ttcagcacgt agtgatcgcc gtcgcgcctc
       61 gcggtggtgc gcagcgcagc ggcgttgctg ccgacgtgcg gctcggtcag gcagaacgcg
      121 ccgagcgccg cgccgctggc cagcggcttc aggaagcgtt gcttctgcgc ctcgtccgcg
      181 tatcccatca ggatcgcgca caccgggcag ttgttgacgc tgatgatcgt cgatacgccg
      241 ccgtcgccgg cggcgatttc ctccagcgcc acggcgagcg cggtgtagtc catgccggcg
      301 ccgccgtact gctccggcac cgcgatgccg aacgcgccga gcgccgccag ttcccgcagc
      361 gcgtccttcg gaaacgtgtg gtcgcggtcc cactgtgccg caaacggtgc cagccgcgac
      421 tgcgcgaatg cgcgcaggct gtcgcgcaac aggtggtgct cttctttcag catggtcgac
      481 ctcgtcaggt cttcagcacc caggcgcggc cacgccggac gatcacccag cccaccagtg
      541 cgccggcaac cagtgcggcc agcagcatcg gcccaccgcc gacgaagccc gtcagcagca
      601 gcacggtcga cagccagttg aagaagtcct cgatcggaaa ccagaccgcg gtggcaagcg
      661 ccgagaagcc gaaccacgtc agcagcgcga ccagcagccc gcgcgtcatc gacgggccga
      721 ctcgctgcac gcgctgctcc gcgccctggt agacgatcag cgcgaagaga ccggcgaaca
      781 gtcccggcac cgccacgaat acccagccgt tcacgccgct ttcgatcgcg aattcgcgca
      841 gcgtgaatcg caggctggcg gccgcgacgc cgccgcagat cccgaacagc agtgcggcga
      901 tcagcggcag cgacagatgc ctcacgcggc ggttcccctc ggctggtttc gacggctcgc
      961 gaatgcgggc cgcattgtcg cgcgtttcag cgccgccggc acgcggtgac ttcgatctcg
     1021 atcgccatgc gcggatcgat cagtccgcac acgattgcgg tggcggcggg gcgcgcgcgg
     1081 ccgaacactt cgccgaagat cggggcgagc cgctcgaaca gggccgcgtc ggtcaggtag
     1141 tagtgcacgc gcacgacatc gtcgagcgtg cagtcggcct caccgagcgc ggccgcgatg
     1201 ttgcggaagc attgccggca ttgcgcgacc gggtcgggct cgatcgtcat cgtcgcgtag
     1261 tcgaagccgg tcgtgcccga cacgtggacg gtgtcgccgt cgaccacggc gcggctgtag
     1321 cccgccaccg cctcgaacga cgacccggac gagatcaacc gtcgctgcat gtcaacctca
     1381 ccaggagaga cgttcgcccg tgtagtcgaa aaactggccg ttgtgcgagg cgctcgccgc
     1441 agcgatcacg cgccgcatgc cggccacgct gtcgcgtacg tcgacctcgc cctcgggtcc
     1501 gcccatgtcg gtgcgtaccc agccgggatg aaacgccatg cacaccacgc cgcgcgcccc
     1561 cagttcgatc gaggcagccc tcagcacgtt gttcaaggcc gccttgctgg cgcggtacag
     1621 ccagctaccc gccgccgtca tcagcgaact cgaccccatc cggctcgaca tcacggcgag
     1681 cttgcccttt gcccgcagga gcgacggcgc gaggatcggc aagagctgca tcggcgcgcg
     1741 cacgttggtg tgcatcacgt ggtcgaagtc gcgcggcgtc ggcgcgacca gcccgtccgt
     1801 gcgcgggcgg atcgacacgc cggcgttgag gatggccaga tcgaccgaat cgccgtccag
     1861 gtgccacgcc agcgcggcga tgccggtttc gtcggtgacg tcgagctcga tcgtcttggc
     1921 gccgagggcg cgcagcttgc cggcatcgtc cgggttgcgg tgcgtggcga tcacgcggtc
     1981 accgtcatcg gcgtactggc gcgcgaattc gaagccgatg ccgcgcgagg cgccgatgac
     2041 gaggacggtg ggcatgcgcc gggcgcttac atcatctcca atgccagcgc cgtcgcttcg
     2101 ccgccgccga tgcacagcgc ggccacgccg cgcttgccgc cggtcttctt cagcgcgccc
     2161 agcagcgtga cgacgatgcg cgcgccggaa gcgccgatcg ggtgtccgag cgcgcaggcg
     2221 ccgccgtgga tgttgacctt ctcgtgcgga atcctgtgct ccttcatcgc cgccatggtc
     2281 acggcggcga aggcctcgtt gacctcccac aggtcgacct gctccgcctt ccagccgact
     2341 ttcttcagca ccttgtcgat cgcgccgacc ggcgccgtgg tgaaccactc gggtgcctgc
     2401 gcgtgcaccg catgtgccag cacgcgcgcg atcggcttgc agcccatctt gtcggccgtc
     2461 gactgccgca tcatcaccag cgccgccgca ccgtcggaaa tcgacgaggc gttggcggcc
     2521 gtgatcgtgc cgtccttctt gaatgcgggc ttgagccccg ggatcttgtc gagcttggcc
     2581 ttgaacggct gctcgtcctt gtcgatcttc acttcgccgc ccttgccggg gatcatgacc
     2641 ggcgcgatct cccacgcgaa gctgccgtcg gtgttggcct tgagcgcgcg ctcggtcgag
     2701 gcgatcgcga acttgtcctg cgcctcgcgc gtgaattcgt acttggccac gcactgctcg
     2761 gcgaacaccc ccatcgcctt gccgcgctcg taggcgtctt ccagcccgtc gagcgccatg
     2821 tggtcgtaca tcgtcgccgc gccgtagcgg tagcccttgc gcgcgccgac cagcaggtgc
     2881 ggggcgttgg tcatgctctc catgccgccg gtgacgagca cgttggccga gcccgcgagc
     2941 agcatgtcgt gcgcgaacat catcgtgcgc atgcccgagc cgcacatctt gctcagggtg
     3001 accgcgccag ccgatgccgg cagcccggcg agcagggtgg cctggcgcgc gggcgcctgg
     3061 ccctggccgg ccatcaggca gcagccgaac agcacttcgt cgatcgcgtc gcggctgacg
     3121 ttggcgcgtt cgaccgcggc cttgatcgcg accgcgccga gttgatacgc ctgctgcgat
     3181 gagaaatcgc cgagcaaacc gccgatcggt gtgcgggcgg ccgagacgat gacaatggat
     3241 tcgctcatgg tatgacctcc tcaggaatgc gtaaccacca ttgttccaga aacgcaaggc
     3301 cgccgacggc gctacgatca aatcctgtca tcgcgcgagg cagccatggc cgacggtccc
     3361 gacttcaagc tgacctattc gacgatgttc gacccgccgc ccgcgctgca ccagcgcttc
     3421 gaggcggcgc tggcgcgcgt gcgcgggcaa ctcggccagg actatcccct gtggatcggc
     3481 gggcgcgagc ggctggccga tacgaccttc gaagtgcgct cgccgatcga ccgcgactgg
     3541 ctgatcggct gcttccaggc gggcacggcc aggcacgcgg atgccgcgct cacggcggcg
     3601 cgcgaagcct ttcccgcctg ggccgcgcgg ccctggcagg agcgcgtgcg gctgctgcga
     3661 cgtgcggcgg cgctgatcga ggagcgcgtg tacgacatcg gcgccgcggt cgcgctcgag
     3721 gtcggcaaga accgcatgga atcccttggc gaggttcagg aaaccgcgga tctgatcgcg
     3781 tggtactgcg accagatgga ggccaacgcc ggtttcgcgc gcgagttacc gaacgaccca
     3841 ttggcgggat tggtcagccg caaccgcacc gtgctgaaac cgtacggccc ctgggtcgtg
     3901 atcgcgccgt tcaatttccc gttcgcgctc gccggcgggc cgatcggcgc ggcgctcgtc
     3961 gccggcaaca cggtggtgtt caaggtggcg agcgacacgt cgctgtccgg ctggctgctg
     4021 ctcgaggcac tgcgcgatgc ggggctgccc gacggcgtgg tcaacttcgt caccggcgcc
     4081 ggcggcgagg tcggcaacgc gctgatcgcg cacggcgacg tggcgggcgt gaccttcacc
     4141 ggctcgtacg aagtcggcat gggcatcgtg cgcgcgttcg cgcagcggcc ctggccgcgc
     4201 cccagcatcg ccgagatggg cggcaagaac gcggcgatcg tcagccgcca cgcggacatc
     4261 gagcgcgcgg cgctcggcat cctgcgctcg gcgttcggcc tgcaggggca gaagtgctcg
     4321 gcctgctcgc gcgtgtacgt cgagcggccg ctcgccgatc cgctgcgcga aaggctggtc
     4381 gagtcggcac gcgcgatcaa ggtgggcgac ccgacgctgc gcgagaactg gatggggccg
     4441 gtgatcaacg cggcagcgct ggcgcgctac gagaaatgcg cgcagcagct gaagcagcac
     4501 ggcgcagtgt tcttcggcgg ggagatgctg aagcgcgacg ggctggaacg cgggtatttc
     4561 gtcgcgccca cgatcggcac ggcgccgctc gactaccggc tgtggcgcga ggaaaagttc
     4621 atcccgctgg tgctggtggc cgaggtcgac tcggtcgacg acgcgctgcg gctggccaat
     4681 cagtcggagt acgga
//
LOCUS       DBNHBY010000150         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_346538, whole genome shotgun sequence.
ACCESSION   DBNHBY010000150 DBNHBY010000000
VERSION     DBNHBY010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_346538"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16HB_03745"
     CDS             <1..252
                     /locus_tag="AC16HB_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5605933.1"
                     /translation="LRRPLLPPDEAAAQPYTPAEAAIIERLREKALVGTADRVAARLR
                     ELAQRLSLDELVIVTWTYDVAPRMRSYELLASAFGLQPR"
     gene            519..1481
                     /locus_tag="AC16HB_03750"
     CDS             519..1481
                     /locus_tag="AC16HB_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_066336475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR5605934.1"
                     /translation="MSDGVYVGIDVASQTLEVYCSAPPKTWQVSNEASGIEALVGELH
                     ALQPALVVLEATGGYEFNAAFALQAAGLQVAVVNPRMARDFARAMGVLAKTDALDARM
                     LAAFARVVDERPDRDRFVKPLADVELQRLQALVMRRRQLIQMMVGEQHRLRIAHAAAH
                     PSIERVVSFLKKELADTDTSVAEHVERHHAELAEALRSVPGIGAASVAVVLAELPELG
                     KLDRRRIAALVGVAPLNRDSGKLRGQRSTWGGRASVRATLYMATLSAIRYNPQIKATF
                     QRLSATGKPAKVSLVACMRKLLTILNAIAKKRSRWNPNLTPTSA"
     gene            complement(1611..>1800)
                     /locus_tag="AC16HB_03755"
     CDS             complement(1611..>1800)
                     /locus_tag="AC16HB_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5605935.1"
                     /translation="PNGTSALMMAVREGKVSTVSLLLAKGADVNQKNQQGGTALAWAT
                     RGNEQGMAAQLRRAGAKE"
BASE COUNT          283 a          586 c          615 g          316 t
ORIGIN      
        1 cttcgccgcc cgctcctgcc gcccgacgaa gcggccgcgc aaccgtatac gccggccgaa
       61 gcggccatca tcgagcgcct ccgcgagaaa gccttggtcg ggaccgctga tcgcgtggcg
      121 gcgcggctgc gcgagcttgc ccagcggctg tcgctcgacg agctcgtgat cgtgacgtgg
      181 acgtacgacg tggcgccgcg gatgcgctcc tacgagctcc tcgcttccgc gttcggcttg
      241 caaccgcgct agggaacgtc tgcaaaaggc gtccggggcg tcggtggatg acgcgatgcc
      301 ggcgttgcgc cttttgcagc cggaacttcc actgcaagtc aggccaccgc cttgtatctt
      361 ggctgtgagt tcggccggcc cgcgtagccc gtcgccgcct tgggcagact cctagcccgt
      421 ccctaagcat gaggcccgcg ggccggatcg tcctctcttg cagcccgaac ggttgtgaga
      481 acgtcatggt tcgcattgac aaggatggga gcgagaggat gtccgacggt gtgtacgtgg
      541 gaattgacgt ggccagccaa accctggagg tctattgcag cgccccgccg aagacgtggc
      601 aggtcagcaa cgaagcctcg ggcattgagg cactggttgg tgaactgcat gccctccagc
      661 cggctttggt ggtcttggaa gcgaccggtg gctacgaatt caacgccgcc tttgcgctcc
      721 aggcggcggg tttgcaggtg gcggtggtga atccccggat ggcgcgtgat ttcgcgcgcg
      781 cgatgggggt gttggcgaag acggacgcgt tggatgcccg gatgttggcg gcctttgcgc
      841 gggtggttga cgaacggccc gaccgggatc gcttcgtcaa gccgctggcc gacgtggaat
      901 tgcaacggtt gcaagccctg gtcatgcgtc ggcggcagct catccaaatg atggtcggcg
      961 aacagcatcg cctgcgcata gcgcacgccg cggcgcatcc gagcatcgaa cgcgtcgtga
     1021 gcttcctcaa gaaggagctg gccgataccg acacgagcgt ggccgagcac gtcgagcggc
     1081 atcacgctga attggcggaa gcgttgcgca gtgtcccggg gatcggcgcc gccagcgtgg
     1141 ccgtggtgtt ggcggagctg cctgaactgg gcaagctcga ccggcggcgc attgcggcgc
     1201 tggtgggcgt ggctccgctc aatcgcgatt ccggtaagct gcgcggacag cgctcgacct
     1261 gggggggtcg ggcgagcgtg cgcgcaactc tgtacatggc caccctcagt gccatccgct
     1321 acaacccgca gatcaaggcg accttccagc gcctgagcgc caccggcaaa cccgcgaaag
     1381 tcagcctggt ggcctgcatg cgaaagctgc tgaccatcct gaatgccatc gccaaaaagc
     1441 gctctcgttg gaaccccaac ttgaccccca cttccgcttg acagcgaaca cggttgcttg
     1501 gggcggcccg gcggcggccg caggaaaccc gacggaagaa cgcgcgtcgt gcgactgtta
     1561 agccgcctgc gcaggttgtg cagatgttcc ccaggggcgt ccggagtgcc tcattccttc
     1621 gcgcccgcgc ggcgcagctg cgcggccatt ccctgctcgt tgccccgggt tgcccaggcc
     1681 agcgccgtcc cgccctgctg gttcttctgg ttcacgtcgg cgcccttggc caggagcagg
     1741 gacaccgtcg acaccttgcc ctcgcgcact gccatcatga gcgcggacgt cccgttgggc
//
LOCUS       DBNHBY010000151         6338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1294034, whole genome shotgun sequence.
ACCESSION   DBNHBY010000151 DBNHBY010000000
VERSION     DBNHBY010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1294034"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16HB_03760"
     CDS             <1..194
                     /locus_tag="AC16HB_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HIR5605936.1"
                     /translation="VIDRLVAALQAALKDPAAVERFAEFGAQPARPADATPAALQTWL
                     RAETARWSSIVKAAGVRAE"
     gene            212..901
                     /locus_tag="AC16HB_03765"
     CDS             212..901
                     /locus_tag="AC16HB_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605937.1"
                     /translation="MSASKLLFALALAASVQVAAQTVPDTLEQRLLACAACHGRNGEG
                     QRKNEYFPRLAGKPAGYLYNQLLNYRELRRQFPIMNYMVAYLSDAYLAEIAEHYAMLQ
                     PPYPPPARAAPALLARGGRLVTQGDPARNLPACTSCHGTALTGLAPAIPGLVGLYADY
                     VAAQMGAWKEGKRHSIESDCMREVARKLMPEDIGAVSAYLAAQPATGDMKPAPAGSLK
                     LPLECGGLARK"
     gene            898..2181
                     /locus_tag="AC16HB_03770"
     CDS             898..2181
                     /locus_tag="AC16HB_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605938.1"
                     /translation="MKRLAMTRLLCSIACAVVLAACSKQEAPAPVAEQKADTHAEQIV
                     RGQYLAAAGDCYACHTVRGGQPYAGGLEIPTPFGSLFAPNITPDSEHGIGKWSADDFW
                     QAMHEGKSKDGSFLYPAFPFTNYTKVTRADSDAIYAYLMSLKPVAQKRRPNELGFPYN
                     QRQLMAGWRALYFKAGVYQDDPNQSPEWNRGAYLVEGLGHCNACHATRNVLGAISADD
                     DYSGGLIPVQNWYAPSLTSSRDTGLGDWDNREIVDLLRTGLSARGAVFGPMAAVVQHS
                     LQNLTLTDLTAMATYLKTQAQKPAAGALMATGPSGEQVAAMMKAGAKVYTDHCEACHQ
                     RDGKGVARVYPPLVESQTTRSANPINAIRIVLNGGYPPSTEGNARPYGMPPFYQDLSD
                     EQIAAVVTYIRRSWFNQGRPVTAAEVARARGVPAD"
     gene            complement(2185..2634)
                     /gene="aroQ"
                     /locus_tag="AC16HB_03775"
     CDS             complement(2185..2634)
                     /gene="aroQ"
                     /locus_tag="AC16HB_03775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5605939.1"
                     /translation="MSRIVYVLNGPNLDLLGQREPEVYGHATLADVQDECALVAAELK
                     LVLEFRQSNAEHQLIEWIHEARTRAAGIVINPAAFSHTSVAILDALKACACPIIEVHI
                     SNIHAREPFRHHSYVSAVAAGVIVGFGTQGYLFALRRLAALLDQPAS"
     gene            2700..3935
                     /gene="pcaCD"
                     /locus_tag="AC16HB_03780"
     CDS             2700..3935
                     /gene="pcaCD"
                     /locus_tag="AC16HB_03780"
                     /EC_number="3.1.1.24"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-carboxymuconolactone
                     decarboxylase/3-oxoadipate enol-lactonase PcaCD"
                     /protein_id="HIR5605940.1"
                     /translation="MSQDRFDEDLERGLANRRAILGDAWVDRSLANANAFSAEFQNLI
                     SRYAWHEIWGRPGLDPKTRRVIVLAITCALGRWEEFELHLRAALTGGDTALSPDEVKE
                     VLMQAAIYAGVPAANTAFTHAQKLLRELGMTAAAADVLAAAHPGVGRVVRTASAPALH
                     FTVRAPRAPRHTIVLSHALGCDLSMWDALARVLAREHRVIAYDHRGHGGSAAPDGPYT
                     MAQLADDAERLLAEIDAGPVIWIGLSMGGMVGQELALRHPARVKALVIANSAGHYPEA
                     ALALWQQRVDAVRAGGMAAIADMVLGRYFSDAFRAARVATVERIRRRLVTTDASGYLA
                     CCFAVRDVDTLDRLSRLNLPALVLAGELDQGTPVAFSQAIAERLRARLVVIPNAAHLS
                     VVEAPEEFERRVCEFIAAL"
     gene            3949..4536
                     /locus_tag="AC16HB_03785"
     CDS             3949..4536
                     /locus_tag="AC16HB_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605941.1"
                     /translation="MPSDPLPLDRFIPTPDVRERFEIAIDAPADLVMEVAAGFDMQSL
                     SLVRAVFRLRERLMGASHQTRQPQGILDETRSLGWGVLEEEPGRLVICGARCQPWLAE
                     VKFTAIAPEQFAAYAEPDQVKIAWTLEARPMGPATTRFAQETRVLATDAAARIKFRRY
                     WRWARFGIIAIRLLLLPAVRRRAQQRWSALQRAHG"
     gene            complement(4565..5677)
                     /locus_tag="AC16HB_03790"
     CDS             complement(4565..5677)
                     /locus_tag="AC16HB_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HIR5605942.1"
                     /translation="MPAQPGDALADVDTPALILDLDRFETNLGRLMDAARDAGVRVRP
                     HAKTHKSLQIAHRQVAAGAVGVCCQKVGEAEVFLAGGIKDVLVTNEVVGERKLRRLAQ
                     LAAMFAPARVGVCIDDASNLRQLAVICDEEAGHIDVYIEIDVGQNRCGVEHAAAAVEL
                     AREVMSYPCLTLMGLQAYAGSAQHRRGVPERKAAAMMAAHRAREVRNALRDAMLPCEI
                     ITGGGTGTFMYEAWSGVYTEIQPGSFAMMDSDYARNESDPDVPPFEHALFILSTVMSV
                     RGDRATLDAGLKAFATDSGPARLAFPGWHVHMVSDEHTVLTRIDDESPTIHLGAKALM
                     IPSHCDPTVNLHDWIVVVRKNRVEDVWCVDARGAVF"
     gene            complement(5815..>6338)
                     /locus_tag="AC16HB_03795"
     CDS             complement(5815..>6338)
                     /locus_tag="AC16HB_03795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5605943.1"
                     /translation="IALLPALSLAEVDAIAARVRTAKRRGDVAVASIHWGGNWGYAIP
                     AQQRAFAHALIDTAQIDLVHGHSSHHAKGIEVHRDRLILYGCGDFINDYEGIGGHESF
                     RAELGLMLCATLDAASGALTQLALTPTRLQRFRIARATSADAHWMAAMLDRECGPLGS
                     GVAFEGDELRLVW"
BASE COUNT          987 a         2211 c         2178 g          962 t
ORIGIN      
        1 cggtgatcga ccggctggtg gccgcgctgc aggcggcgct gaaggatccg gccgcggtcg
       61 aacgtttcgc cgagttcggc gcgcagcccg caagacccgc cgacgccacg cccgccgcat
      121 tgcagacgtg gctgagggcg gagacggcgc gctggagttc gatcgtcaag gcggcgggcg
      181 tgcgcgccga atagcgcgcc gggagacatg catgtcggcc tccaagctgc tgttcgccct
      241 cgcactcgcc gcatccgtcc aggtcgcagc gcagaccgtg cccgacacgc tcgagcagcg
      301 cctgctcgcg tgcgctgcgt gtcacggcag gaacggcgag ggacagcgca agaacgagta
      361 cttcccgcgg ctggccggca aacccgccgg gtatctgtac aaccagttgc tgaactatcg
      421 cgagctgcgg cgccagttcc cgatcatgaa ttacatggtc gcgtacctgt ccgacgctta
      481 tctggccgag atcgccgagc actacgcgat gctgcagcca ccgtatccgc cgccggcgag
      541 agccgcaccg gcgctgctgg cgcgcggcgg ccggctcgtc acgcagggcg atccggcgcg
      601 caacctgccg gcgtgcactt cgtgccacgg caccgcgctg acgggtctcg caccggcgat
      661 tccggggctg gtggggttgt atgccgacta cgtcgctgcg cagatgggcg catggaaaga
      721 gggcaagcgg cactcgatcg agtccgactg catgcgcgaa gtcgcgcgca agctcatgcc
      781 cgaggacatc ggtgcggtca gcgcatacct ggccgcgcag cccgccaccg gtgacatgaa
      841 gccggcgcct gcaggctcgc tgaaactgcc gctcgaatgc ggcgggctcg cgcgcaaatg
      901 aagaggctcg cgatgacacg cctgctgtgc tcgattgcct gcgcggtcgt gctcgccgca
      961 tgctcgaagc aggaggcgcc ggccccggtt gcggagcaga aggccgacac gcacgccgag
     1021 cagatcgtgc gcggccagta cctcgcggcg gcgggcgact gctacgcgtg ccacacggtg
     1081 cgcggcggac aaccgtacgc cggcggactc gagatcccca cgccgttcgg atcgctgttc
     1141 gcgcccaaca tcacgccgga ttcggagcac ggcatcggca agtggtcggc cgacgacttc
     1201 tggcaggcga tgcacgaggg caaatcgaaa gacggctcgt tcctgtaccc cgcatttcct
     1261 ttcacgaact acaccaaggt cacgcgcgct gactcggacg cgatctacgc ctacctgatg
     1321 tcgctgaagc cggtcgcgca gaagcgccgc ccgaacgagc ttggcttccc atacaaccag
     1381 cggcagctga tggcgggctg gcgggccctg tacttcaagg ccggcgtcta ccaggacgat
     1441 ccgaaccagt cgcccgaatg gaaccgcggc gcctatctcg tcgaggggct gggccactgc
     1501 aacgcttgcc acgcgacgcg caacgtgctc ggcgcgatct ccgccgacga cgactactcc
     1561 ggcgggctca taccggtgca gaactggtac gcaccgtcgc tgacctcgag ccgtgatacc
     1621 gggctcggcg actgggacaa cagggagatc gtcgacctgc tgcgcaccgg actctccgcc
     1681 cgcggcgcgg tgttcggtcc gatggcggcg gtcgtgcagc acagcctgca gaacctgacg
     1741 ctgaccgacc tgaccgcgat ggcgacctac ctgaagacgc aggcgcagaa gcccgctgcg
     1801 ggcgcgctga tggcgaccgg gccgtccggg gagcaggtcg cggccatgat gaaggccggt
     1861 gcgaaggtct acaccgacca ctgcgaggcc tgccaccagc gcgacggcaa gggtgtcgcg
     1921 cgcgtgtatc cgccgctggt cgagtcgcag acgacgcgca gcgccaatcc gatcaatgcg
     1981 atccgcatcg tgctgaacgg cggctatccg cccagcaccg agggcaacgc gcggccctat
     2041 ggcatgccgc cgttctatca ggacctctcc gacgaacaga tcgccgcggt cgtcacctac
     2101 atccgccgct cgtggttcaa ccagggcagg cctgtgaccg cggccgaggt cgcccgcgca
     2161 cgcggtgtgc ccgcggattg atcgctaact ggcaggctgg tcgagcagcg ccgccagccg
     2221 ccgcagcgcg aacaggtagc cctgcgtgcc gaacccgacg atcacgcccg ccgcgaccgc
     2281 ggagacgtac gagtgatgcc ggaacggctc gcgggcgtgg atgttcgaga tgtgcacttc
     2341 gatgatcggg caggcgcaag ccttcagtgc gtcgaggatc gccaccgacg tgtgcgagaa
     2401 cgcggccgga ttgatcacga tgccggccgc gcgcgtgcgc gcctcgtgga tccattcgat
     2461 cagctggtgt tcggcattgc tctgccggaa ctcgagcacg agcttcaact cggccgcgac
     2521 gagcgcgcac tcgtcctgca cgtccgcgag cgtggcgtgg ccatagactt ccggctcgcg
     2581 ctggccaagc agatccaggt tcgggccgtt cagcacgtac acgatgcggc tcattcggtc
     2641 tccccggcga cgtagactgc acggatcata agggccggcg gacgcaagga tctacagcga
     2701 tgagccagga caggttcgac gaagacctcg agcgcgggct cgccaaccgc cgcgcgatcc
     2761 tcggcgacgc gtgggtcgac cgttcgcttg ccaacgccaa cgcgttcagc gccgagttcc
     2821 agaacctgat ctcgcgctac gcatggcacg agatctgggg ccgcccggga ctcgacccga
     2881 agacgcgtcg cgtaatcgtg ctggcgatca cctgcgcgct tggccgctgg gaagagttcg
     2941 aactgcacct gcgcgccgcg ttgactggag gcgacaccgc gctctcgccc gacgaggtga
     3001 aggaagtgct gatgcaggcg gcgatctacg ccggcgtgcc ggccgccaac accgcgttca
     3061 cgcacgcgca gaaactgctg cgcgaactcg ggatgacagc ggctgcagcc gacgtgctcg
     3121 ccgccgcgca ccccggcgtc gggcgcgtgg tacgcacggc gagcgcgccg gcgctgcact
     3181 tcaccgtgcg cgcaccgcgc gcgccgcggc acacgatcgt gctcagccac gcgctcggct
     3241 gcgacctgtc gatgtgggat gcgctcgccc gtgttctcgc gcgtgagcac cgcgtgatcg
     3301 catacgacca ccgcggccac ggcggctcgg cggcgcccga cggaccgtac acgatggcgc
     3361 aactggcgga cgatgcggag cgcctgctcg cggagatcga tgccggcccg gtgatctgga
     3421 tcggcctgtc gatgggcggc atggtcgggc aggaactcgc gctgcgccac ccggcgcgcg
     3481 tgaaggcgct ggtgatcgca aactcggccg ggcactatcc ggaggcggcg ctggcgctgt
     3541 ggcagcagcg ggtcgacgcc gtgcgtgccg gcggcatggc cgcgatcgcc gacatggtgc
     3601 tcggccgcta cttcagcgac gcatttcgcg ccgcgcgcgt ggcgacggtc gaacgcattc
     3661 gccgccgcct cgtgaccacc gacgccagcg gctacctcgc gtgctgcttc gcggtgcgtg
     3721 acgtcgatac gctcgaccgg ctgtcgcgat tgaacctgcc cgcgctggtg ctcgcgggcg
     3781 aactcgacca gggtacgccg gtcgcgttct cgcaggcgat tgcggagaga ctgcgcgcca
     3841 ggctggtcgt gattccgaac gccgcccacc tgagcgtggt cgaggcgccg gaggaattcg
     3901 agcgccgcgt gtgcgagttc atcgcggcgc tgtgatccgg cgccgcgcat gccttccgat
     3961 ccgctgccgc tcgatcgctt catccccacc cccgacgtac gcgagcgctt cgagatcgcg
     4021 atcgacgcgc cggccgatct ggtgatggag gtcgcggccg gcttcgacat gcagtcgctg
     4081 tccctggtca gggccgtgtt ccggctgcgc gagcggttga tgggagcgtc gcatcagacg
     4141 cgccagccgc agggcattct ggacgagacg cggtcgctgg gctggggtgt gctggaggag
     4201 gaaccgggac ggctggtcat ctgcggtgcg cggtgccaac cctggctggc cgaggtgaag
     4261 ttcaccgcaa tcgcgcccga acaattcgcc gcgtacgcgg agccggatca ggtgaagatc
     4321 gcgtggacgc tggaagcgcg gccgatgggt ccggcgacca cgcgcttcgc gcaggagacg
     4381 cgcgtgctcg ccaccgacgc ggcggcgcgc atcaagttcc gccgctactg gcgctgggcg
     4441 cgcttcggga tcatcgcgat ccgacttctg ctgctgccgg ccgtgcggcg ccgcgcgcag
     4501 caacgctggt ccgccctgca gcgcgcgcac ggctgaacct cgaccgggtc acgggccgcg
     4561 cgcctcagaa cacggccccc cgcgcatcga cgcaccagac gtcctcgacc cggttcttgc
     4621 gcacgacgac gatccagtcg tgcagattca ccgtcgggtc gcagtggctc gggatcatca
     4681 gcgccttggc gccaaggtgg atagtcgggc tttcgtcatc gatgcgcgtc agcaccgtgt
     4741 gctcgtcgct caccatgtgt acgtgccagc cgggaaaagc caggcgcgcc gggccggagt
     4801 cggtcgcgaa cgcctttagt cccgcatcca gcgtggcgcg gtcgccgcgc acgctcatca
     4861 ccgtcgacaa gatgaacaga gcgtgctcga acggcggcac gtcggggtcg gattcgttgc
     4921 gtgcgtagtc cgaatccatc atcgcgaacg agccgggctg gatctcggtg tacacgccgc
     4981 tccacgcttc gtacatgaac gtgccggtgc cgccgccggt gatgatctcg cacggaagca
     5041 tggcgtcgcg cagcgcgttg cgcacttcgc gcgcgcgatg cgccgccatc atcgccgccg
     5101 ccttgcgctc gggcacgccg cgccggtgct gcgcgctgcc ggcgtaggcc tgcagcccca
     5161 tcaaggtcag gcacggatag ctcatcacct cgcgcgccag ctcgaccgcg gccgcggcgt
     5221 gctccacgcc gcagcggttc tggccgacgt cgatctcgat gtagacatcg atgtgccccg
     5281 cctcctcgtc gcagatcacc gcgagctggc gcaggttcga ggcatcgtcg atgcagacgc
     5341 cgacgcgcgc cggcgcgaac atggccgcca gttgcgccag ccgtcgcagc ttgcgctcgc
     5401 cgacgacttc gttggtgacc agaacgtcct tgatgccgcc ggcgagaaat acttcggcct
     5461 cgccgacttt ctggcagcac acgccgaccg cgccggcggc gacctgccgg tgcgcgatct
     5521 gcagcgactt gtgcgtcttg gcgtgcggcc gcacgcgcac gccggcgtcg cgcgcggcat
     5581 ccatcaggcg ccccaggttc gtctcgaacc ggtcgaggtc gaggatcagc gctggcgtgt
     5641 cgacgtccgc cagcgcgtct cccggttgcg cgggcatcca gtgcaaagtc atatcgcggt
     5701 tggttggtcc gcgcgcactg ccgtgccgct tcgctgcgcg gagggcggag tctagccggc
     5761 tcccgtcgcg gcgacccgcc actgcgcctt cgcgggatac accgcgcggc gcattcacca
     5821 caccaggcgc agttcgtcgc cctcgaatgc cacgccggag cccagcggcc cgcattcgcg
     5881 gtcgagcatc gcggccatcc agtgtgcatc cgcggacgtg gcgcgtgcga tgcggaaacg
     5941 ctgcaatcgc gtcggcgtca gcgcaagctg tgtcagcgcg ccgctggcgg cgtcgagcgt
     6001 ggcgcacaac atcagtccga gctcggcgcg aaacgattcg tgtccgccga tgccttcgta
     6061 gtcgttgatg aaatcgccgc agccatacag gatcaaccgg tcccggtgca cctcgatgcc
     6121 cttcgcgtgg tgcgacgaat ggccgtgcac caggtcgatc tgcgccgtgt cgatcagcgc
     6181 gtgtgcaaag gcgcgctgct gcgccggtat cgcgtaaccc cagttgccgc cccagtggat
     6241 cgatgccacc gcgacgtcgc cgcgccgctt cgcggtgcgc acgcgtgcgg cgatcgcgtc
     6301 gacttccgca agcgacaacg ccggcagcag cgcaatac
//
LOCUS       DBNHBY010000152         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1632110, whole genome shotgun sequence.
ACCESSION   DBNHBY010000152 DBNHBY010000000
VERSION     DBNHBY010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1632110"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="AC16HB_03800"
     CDS             complement(<1..912)
                     /locus_tag="AC16HB_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5605944.1"
                     /translation="MKKNTLLVTGGRDRETFDFVNPPLTRGSTVLHESVADMRERVRR
                     RNAGDDAEPVAYGIYGTPTHQAFYEALTALEGGYRSWALPSGLTACTMAILAYVQRGD
                     HLLVPDSVYWPTRRFCRDTLPRYGVATTFYDPRIGAEIEQFFTPATRVLFLESPGSLT
                     FEMQDVPLLASIARARDAVSVIDNTWATPLFFQPLKVGVDVSVHAATKYIGGHSDLLM
                     GTVTCNERAWPRMRETLHHYGLTTSPDDCYVALRGLRSMAARLVQHRATAERLIAWLK
                     RQPEVERVLYPADPDDPGHALWKRDMSG"
     gene            complement(909..1988)
                     /locus_tag="AC16HB_03805"
     CDS             complement(909..1988)
                     /locus_tag="AC16HB_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5605945.1"
                     /translation="MNPNESELTYPWADVPAPGTVQKLRPGVHWLRMRLPFALDHINL
                     WLFDDEIDGVRGWTIVDCGITSDETRAAWERIFDTVFDGRPLLRVISTHFHPDHLGLA
                     WWLTQGGDRGRWTAPLWITATEYSVGRFLSLGADDTSGESAALHFRRNGLADPDALAK
                     VRARGGGYYPKLVPAVPANYRRIWNDEEIAIGPRGARRVFKVIIGYGHAPEHVSLYCA
                     EDELLISGDMVLPRISTNVSVFDVEPDGDPLPRYLRSLDRYLMLPADTLVLPSHGKPF
                     LGLHRRVTQQHMHHQARLDEVLAACSQPRTAAEIVPVLFRREFDVHQLTFALGESLAH
                     LHALWHEGKLARSLRADGVYTFQAT"
     gene            2059..2481
                     /locus_tag="AC16HB_03810"
     CDS             2059..2481
                     /locus_tag="AC16HB_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5605946.1"
                     /translation="MPATRPEAKPPKDPGTLTITDLAREFDITPRAIRFYEDHGILQP
                     TRAGSSGLKRIYSPRDRTRLKLTLRGKRLGLTLAQVRELIDMYESPKDAVAQAKRFLA
                     VLTQHRAALEQQREDIEVTLSEIERHEADCRRILGTRR"
     gene            2623..3081
                     /locus_tag="AC16HB_03815"
     CDS             2623..3081
                     /locus_tag="AC16HB_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR5605947.1"
                     /translation="MLKSILELIEGRTTGQRTQDPRAVELATAALLVEVVHAARDVLP
                     TEQQALRRAMHEKFGLDTPQADELLLDAEERMRQATDYYQYTSLINANFTHEQKQQVI
                     ELMWRVAYADDVLSAQEQHLIRKIAGLLHVPDGEYIAAKLRAKADAERAR"
     gene            complement(3085..>3305)
                     /locus_tag="AC16HB_03820"
     CDS             complement(3085..>3305)
                     /locus_tag="AC16HB_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HIR5605948.1"
                     /translation="AAMLDGATDEAQARRLARDTALVLQAALLRQHSTDAMFAAFCAS
                     RLDDAADVFGALPAGCELDAIIARAQPH"
BASE COUNT          556 a         1128 c         1141 g          480 t
ORIGIN      
        1 gccgctcata tcccgcttcc aaagcgcatg gccgggatcg tcgggatcgg ccgggtacag
       61 cacgcgctcg acctcgggct gccgtttcag ccacgcgatc aaacgttccg cggtcgcgcg
      121 gtgctgcacc agtcgtgccg ccatcgagcg caacccgcgc agcgcgacat aacagtcgtc
      181 ggggctggtc gtcagcccgt agtggtgcag cgtctcgcgc atgcgcggcc acgcgcgctc
      241 gttgcaggtg acggtcccca tcagcaggtc cgagtggccg ccgatgtatt tggttgccgc
      301 atgcaccgat acgtccacgc cgaccttcag cggctggaaa aagagtggcg tggcccaggt
      361 gttgtcgatg acgctcaccg cgtcgcgtgc gcgcgcgatc gaagccagca gcggcacgtc
      421 ctgcatttcg aacgtcagcg atcccggtga ctcgaggaac aggacgcgtg tggccggcgt
      481 gaagaactgc tcgatttcgg cgccgatccg cgggtcgtag aacgtggtcg caactccgta
      541 acgcggcagc gtgtcgcggc agaaccggcg cgtcggccag tagaccgaat cgggcacgag
      601 caagtggtcg ccccgctgca cgtacgcgag gatcgccatc gtgcaagcgg tcaggcccga
      661 cggcagtgcc cacgagcgat agccgccttc gagcgcggtc agcgcctcgt agaacgcctg
      721 gtgcgtcggc gtgccgtaga tgccgtaggc gaccggctcg gcgtcgtcgc cggcattgcg
      781 ccggcgcacg cgctcgcgca tgtcggccac gctctcgtgc agcacggtcg agccgcgcgt
      841 cagtggcgga ttgacgaagt cgaacgtctc gcgatcgcgg ccgccggtga cgagcagcgt
      901 gttcttcttc atgtcgcctg gaacgtgtag acgccgtcgg cacgcaggct tcgcgcgagc
      961 ttgccctcgt gccacagcgc gtgcagatgc gcgagagatt cgccgagcgc gaaagtcaac
     1021 tggtgcacgt cgaactcgcg cctgaacagc accggcacga tctcggccgc ggtgcgcggc
     1081 tggctgcagg ccgcgagcac ctcgtcgagc cgcgcctgat ggtgcatgtg ctgctgcgtg
     1141 acgcgccggt gcaggcccag gaacggcttg ccgtgcgacg gcagcaccag cgtgtcggcc
     1201 ggcagcatca ggtagcggtc gagcgagcgc aggtagcgcg gcagcggatc accgtcgggc
     1261 tcgacgtcga acacgctgac gttggttgag atgcgcggca gcaccatgtc gccggatatc
     1321 agcagttcgt cctcggcgca gtagagcgac acatgctcgg gcgcgtggcc gtagccgatg
     1381 atgaccttga acacgcgcct ggcaccgcgc ggaccgatcg cgatctcctc gtcgttccag
     1441 atgcggcggt agttggccgg caccgcgggc acgagcttcg ggtagtagcc gccaccgcgt
     1501 gcgcgcacct tggcaagcgc gtcggggtcg gccagcccat tgcggcggaa atgcagcgct
     1561 gccgattcgc cgctggtgtc gtcggcgccg agcgacagga agcggccgac cgagtactcg
     1621 gtcgccgtga tccacaaggg cgcggtccac cggccgcggt ctccaccctg cgtcagccac
     1681 catgccagcc ccaggtgatc cggatggaaa tgcgtgctga tcacgcgcag gagcggccgg
     1741 ccgtcgaaca cggtgtcgaa gatcctctcc cacgccgccc gcgtttcgtc cgacgtgatg
     1801 ccgcagtcga cgatcgtcca gccgcgcacg ccgtcgatct cgtcgtcgaa cagccacagg
     1861 ttgatgtggt cgagcgcgaa cggcaatcgc atccgcagcc agtgcacgcc cggccgcaat
     1921 ttctggaccg taccgggcgc cggtacgtcg gcccacggat acgtgagttc gctttcgttg
     1981 ggattcacgg cggcaacgtt cgacttgacg ttcgcgtcaa ctgcgccgat tctacgcggc
     2041 gaatcccgat caccgccgat gcccgcgacc agacccgaag ccaagccgcc gaaagacccc
     2101 ggcacactca cgatcaccga cctggcgcga gagttcgaca tcacgccgcg cgcgatccgc
     2161 ttctacgagg accacggcat cctgcagccg acgcgcgcgg gcagctcggg cctgaagcgg
     2221 atctactcgc cgcgcgaccg cacgcggctg aagctcacgc tgcgcggcaa gcggctgggg
     2281 ctgacgctgg cgcaggtgcg cgagctgatc gacatgtacg agtcgcccaa ggacgcggtc
     2341 gcgcaggcga agcgtttcct cgcggtgctg acccagcacc gcgcggcgct cgagcagcag
     2401 cgcgaggaca tcgaggtcac gctgtcggag atcgagcgcc acgaggccga ttgccggcgc
     2461 atcctcggca cgcgccgctg actgcgcgcc cgccgcggcc ggtgcattag gcggtggtta
     2521 agcgcgcgaa ccatccagcg ccgcgatggt cgatggtatg ccccttcggc ccgaagcgcc
     2581 gcgtcgccgt ggcaggtcgg ggcagaacgg agccgcaccg cgatgctgaa atcgatcctg
     2641 gaactcatcg aaggccgcac gaccgggcaa cgcacgcagg acccgcgcgc ggtcgaactg
     2701 gccacggcgg cgctgctggt cgaggtggtc cacgccgcgc gcgacgtgct gccgacggag
     2761 cagcaggcgc tgcggcgcgc tatgcacgag aagttcgggc tggacacgcc gcaggctgac
     2821 gagctgctgc tcgacgccga ggaaagaatg cgccaggcga ccgactacta ccagtacacg
     2881 tcgctgatca acgccaactt cacgcacgaa cagaagcagc aggtgatcga gctgatgtgg
     2941 cgcgtcgcgt atgcagacga cgtgttgtcg gcgcaggagc agcacctgat ccgcaagatc
     3001 gccggcctgc tgcacgtacc cgacggcgag tacatcgccg ccaagctgcg cgccaaagcc
     3061 gacgccgaac gggcacgctg acggtcaatg cggctgcgcg cgcgcgatga tcgcgtccag
     3121 ttcgcaaccg gccggcagcg caccgaacac gtcggccgca tcgtccaggc gtgacgcaca
     3181 gaacgccgcg aacatcgcgt cggtggagtg ctggcgcagc agcgccgcct gcaggacgag
     3241 cgcggtgtcg cgtgccagcc ggcgcgcctg cgcctcgtcg gtggcgccgt cgagcatggc
     3301 ggcca
//
LOCUS       DBNHBY010000153         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_685003, whole genome shotgun sequence.
ACCESSION   DBNHBY010000153 DBNHBY010000000
VERSION     DBNHBY010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_685003"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16HB_03825"
     CDS             complement(<1..225)
                     /locus_tag="AC16HB_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605949.1"
                     /translation="MNIATAVMIIRMPRSVCCAGCAESPALIAEGNRTAESRRAMIAL
                     RPLRIANDTTMPAPAVQPDATMVDALRRAGL"
     gene            218..1045
                     /locus_tag="AC16HB_03830"
     CDS             218..1045
                     /locus_tag="AC16HB_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5605950.1"
                     /translation="MFTVLDSIAKYLARFYPVPGVVWARYAFHLLLVVLILGPRLRGR
                     LVRTRRLPLQIVRGVVLAASSMFFVTALKFMPMAEASSITFIAPVLVTLAAALVLKEK
                     VEPARWAAVCAGFIGVLIIIRPGSGVFSAVALLPLATAACFAAYQVMTRTLAGVDDPY
                     TTLFYSGLIGTVLLAVVLPFAWVAPATFSHAALMVLMGLIGGGSHLVLIKAYEYAPAS
                     RLAPFSYTQLLWVLVAGYVVFGDFPDGWSLLGIAVIASSAIYIATHQRFGARRNVAD"
     gene            complement(1042..2013)
                     /locus_tag="AC16HB_03835"
     CDS             complement(1042..2013)
                     /locus_tag="AC16HB_03835"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR5605951.1"
                     /translation="MGAQNGERRRIGVTSAQRGRGRVLLLVLVVVVLGAILWRQRAPG
                     AVAPGASLLQRLNPACADPFPPASTIIAGSGARPQAAAVSSTQFVNRTPVDRIVDLLD
                     GEHVVLSIAVPAHQTATVPLPVGAYGWRLRNGAAWCAATAAFVREQRTTITNGIQIVP
                     TSMLSIHIEPDAAQAAGFTLHASDRPVLANAPASPDSTSTRPLAGGGLLIPRAADGHY
                     YLEGMIDGERVQFMVDTGASGVAIPAELARRLGHYSGPDIVSTTANGPTTGYAIRVAR
                     VAFGPFFADDVRVVALPALDKPLLGMSLLQSLDIRQTAAGLELRPAR"
     gene            2166..>2701
                     /locus_tag="AC16HB_03840"
     CDS             2166..>2701
                     /locus_tag="AC16HB_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR5605952.1"
                     /translation="MMGVVHDPLANKGTAFSVAEREGLGLTGFLPPRVESIDEQVERV
                     LAAVRAKSTPLEKYCALAALQNENETLYFRALIDHLAELVPIVYTPTVGQACLDWSRI
                     YERPRGLYLTLRERGRIRDVLKRWPRQEVGIIVVTDGGRILGLGDLGANGMGIPIGKL
                     ALYVACAGVDPARCLPVML"
BASE COUNT          378 a          897 c          968 g          458 t
ORIGIN      
        1 caatccggcg cggcgcagcg catcgaccat cgtcgcgtca ggctggacgg caggcgcagg
       61 catggtcgtg tcgttcgcaa tgcggagcgg ccgcaaggct atcatcgcgc gccgtgactc
      121 cgccgtccga tttccttccg cgatgagcgc gggcgattcc gcgcagccgg cgcagcacac
      181 gctgcgcggc atcctgatga tcatgacggc ggtggcgatg ttcaccgtcc tggattccat
      241 cgccaagtac ctggcgcgct tctatccggt gcccggtgtc gtctgggcgc gctacgcgtt
      301 tcacctgctg ctggtcgtgc tgatcctcgg gccgcggttg cgcggccggc tggtgcgcac
      361 gcggcggttg ccgctgcaga tcgtgcgcgg cgtggtgctc gcggcgtcga gcatgttctt
      421 cgtcaccgcg ctcaagttca tgccgatggc ggaagcctcg tcgatcacct tcattgcccc
      481 ggtgctggtg acgctggcgg cggcgctggt gctgaaagag aaggtcgagc cggcacgctg
      541 ggcggcagtg tgcgcgggct tcatcggcgt gctgatcatc atccgtcccg gcagcggcgt
      601 tttcagcgcg gtggcgctgc tgccgctggc caccgcggcg tgcttcgcgg cctatcaggt
      661 gatgacgcgc acgctggccg gcgtggacga cccatacacg acgctgttct attccggcct
      721 gatcggcact gtgctgctcg cggtcgtgtt gccgtttgcg tgggttgcgc cggcgacgtt
      781 ctcgcacgcg gcgctgatgg tgctgatggg tctgattggc ggcggcagcc acctggtgct
      841 gatcaaggcg tacgagtacg cgccggcctc gcggctggcg ccgttctcgt acacgcaact
      901 gctgtgggtg ctggtcgccg gttacgtggt gttcggtgat ttccctgacg gctggtcgct
      961 cctcggcatc gcggtgatcg cgtcgagcgc gatctacatc gcgacccatc agcgcttcgg
     1021 tgcgcgccgg aacgtggcgg actagcgcgc cggtcgcagt tcgagcccgg cggcggtctg
     1081 ccggatgtcc agcgactgca gcagtgacat gccgagcagc ggtttgtcga gcgcgggcag
     1141 cgcgacgacc cgcacgtcgt cggcgaagaa cgggccgaat gccacgcgcg caacccggat
     1201 cgcatagccc gtggtcggcc cgttggcggt ggtcgagacg atgtcggggc ccgagtaatg
     1261 accgagccgg cgcgcgagtt cggccggtat cgccacgccg ctcgcgccgg tgtcgaccat
     1321 gaactgcacg cgctcgccat cgatcatgcc ttcgaggtaa tagtggccgt cggccgcgcg
     1381 cgggatcagc aggccgccac ccgcgagcgg gcgggtcgac gtcgaatccg ggctggcggg
     1441 agcgttcgcc agcaccggcc ggtcggaggc gtgcagcgtg aatccggccg cttgcgctgc
     1501 gtccggctcg atgtgaatcg agagcatcga cgtcgggacg atctggatgc cgttcgtgat
     1561 ggtcgtccgc tgctcgcgca cgaacgcagc ggtcgccgcg caccacgccg caccgttgcg
     1621 cagtcgccac ccataggcgc cgaccggcaa aggcaccgtt gctgtctggt gcgccggcac
     1681 ggcgatgctc agcaccacgt gttcgccgtc aagcagatcg acaatgcgat cgaccggcgt
     1741 gcggttgacg aattgcgtgc tcgagaccgc ggccgcctgg ggccgcgcgc cgctgccagc
     1801 gatgatcgtg ctcgcaggcg ggaacggatc ggcgcacgcc ggattcagcc gttgcagcag
     1861 gctggcgccc ggcgcgaccg caccgggcgc gcgttggcgc cacaggatcg cgccgagcac
     1921 gacgaccacg agcacgagca gcagcacgcg accgcggccg cgctgcgccg aagtgacgcc
     1981 aatgcgcctt cgttccccgt tctgcgcgcc catcgagtcc ttcctcgcag ccgatatcgg
     2041 ccgagcttag aggaccgtcc ggggacgcgt gaatccgccg tccggcgccg cgccctgcca
     2101 attcgcaacg tgcgtcggcg cggaatacct acgatgaatg cacgctgatg atggggagaa
     2161 cggcgatgat gggcgttgtg cacgatccgt tggcgaacaa gggcacggcg ttttccgtcg
     2221 cggagcgcga agggctgggc ctcaccggct ttctgccgcc gcgcgtcgag agcatcgacg
     2281 agcaggtcga gcgcgttctc gcggcggtgc gcgcgaagtc gaccccgctg gaaaagtact
     2341 gcgcgctggc ggcgctgcag aacgagaacg aaacgctgta cttccgtgca ctgatcgacc
     2401 acctggccga actggtgccg atcgtctaca cgccgacggt cgggcaggcc tgtctcgact
     2461 ggagccgcat ctacgagcgg ccgcgcgggc tgtacctgac actgcgtgag cgcggccgca
     2521 tccgcgacgt gctgaagcgc tggccgcgcc aggaagttgg catcatcgtc gtcaccgacg
     2581 gcggccgcat cctggggctg ggcgacctcg gcgccaacgg catgggcatc ccgatcggca
     2641 agctggcgct ctatgtggcg tgcgccggcg tcgatccggc gcgctgcctg ccggtgatgc
     2701 t
//
LOCUS       DBNHBY010000154         1518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1023198, whole genome shotgun sequence.
ACCESSION   DBNHBY010000154 DBNHBY010000000
VERSION     DBNHBY010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1023198"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1363
                     /locus_tag="AC16HB_03845"
     CDS             179..1363
                     /locus_tag="AC16HB_03845"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HIR5605953.1"
                     /translation="MKIDQYETDALMDVAARPPLVFVEGRGSWLVDQRGKRYLDFVQG
                     WAVNALGHCPHEIAAALAAQSQRLISPSPAFFNDAAIRLARRLAELSGLQRVFLASSG
                     AEANEGAIKLARKYGSLHKDGAFEIVTFADGFHGRTLATMAASGKAGWDRLFEPKVPG
                     FPKARLNDLDSVRACIGPNTVAVMLEPVQGEAGVVPATAEFLRAVRALCDEHGLLLIL
                     DEVQTGVGRTGTLFAFQQAGIAPDIMTLGKGLGGGVPLAALLARQEAACFEHGDQGGT
                     YCGNPLMAAVGSAVLEAVAQPEFLGGVAERGMHLQRHLQQLIGRHRLVGERGAGLLRA
                     LDLGRPIASAVVEAARELQPEGLLLNAPRPHLLRFMPALNVAFEEIDRMSALLDGVLH
                     DI"
BASE COUNT          244 a          489 c          558 g          227 t
ORIGIN      
        1 aagctgacct ggcttgatcc tgaggtatag tgcacgccat gacgcacatg actagccgac
       61 gcagccgacg ccatgcggaa ctccgcaggg gagcgcgcgc gtaggacacg tcaccggaaa
      121 cggttgaccc aacgaaggcc gcggaccccg cggccttttt gctttcaagg agccgaagat
      181 gaagatcgat cagtacgaga ccgatgccct gatggacgtc gcggcgcgtc cgccgctggt
      241 gttcgtcgag ggtcgaggtt cgtggctggt cgatcaacgc ggcaagcgct acctggactt
      301 cgtgcagggc tgggcggtca acgcgctggg gcattgcccg cacgagatcg ctgcggcgct
      361 cgccgcgcag tcgcagcgac tgatctcgcc cagccccgct ttcttcaacg atgccgcgat
      421 ccggctcgcg cggcggcttg cggaactgtc cggattgcag cgcgtgttcc tcgcatcgag
      481 cggcgcggag gcgaacgagg gcgcgatcaa gctggcgcgc aagtacggat cgctgcacaa
      541 ggatggcgcg ttcgagatcg tcaccttcgc cgacggcttt cacggccgca cgctggcgac
      601 gatggcggcg agcggcaagg ccggctggga tcggctgttc gagccgaaag tgccgggatt
      661 cccgaaagcg cggctgaacg atctcgactc ggtgcgcgcc tgcatcggac cgaacacggt
      721 tgcggtgatg ctcgagcccg ttcaaggcga ggccggcgtc gtaccggcga cggcggagtt
      781 cctgcgcgct gtgcgcgcgc tgtgcgacga gcacgggctg ctgctcatcc tcgacgaggt
      841 gcagaccggc gtgggccgga ccggcacgct gttcgcattc cagcaggcgg gcatcgcccc
      901 cgacatcatg acgctgggca aggggctcgg gggcggcgtg ccgctggccg cgctgctcgc
      961 ccggcaggag gccgcgtgct tcgagcacgg cgaccagggc ggcacctatt gcggcaaccc
     1021 gctgatggcg gcggtcggaa gcgcggtgct cgaggccgtc gcgcagccgg agttcctggg
     1081 cggcgtcgcc gagcgcggca tgcacctgca gcggcatctg cagcagctca tcggcaggca
     1141 tcggctcgtc ggcgaacgcg gcgcggggct gctgcgtgcc ctcgacctcg gccggccgat
     1201 cgcgtcggcg gtggtcgagg cggcgcgcga actgcagccc gaggggctgc tgctgaacgc
     1261 gccgcggccg catctgctgc gattcatgcc ggcactcaat gtcgcgttcg aggagatcga
     1321 ccgcatgtcc gcattgctcg acggggtgct gcatgacatc tgaaatcggg aacgtgcgcc
     1381 ggggcgggca gaatggcgct gaagacaagg gagatgcggc atgagcaagg cgaccctcta
     1441 catcgacggc gaacacggca ccacggggct cgagatccgc gagcggctgg ccggccgcac
     1501 cgacgtcgaa ctcgtcag
//
LOCUS       DBNHBY010000155         4515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1429128, whole genome shotgun sequence.
ACCESSION   DBNHBY010000155 DBNHBY010000000
VERSION     DBNHBY010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1429128"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /locus_tag="AC16HB_03850"
     CDS             complement(<1..2089)
                     /locus_tag="AC16HB_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5605954.1"
                     /translation="MFSRFFIDRPIFAAVLSIFIVLAGLAAMRILPIAQYPEIAPPVV
                     TVAAVYPGASAQVLESTVAAPLENAINGVEGMLYMSSNSASNGVVQIQVTFEIGTHID
                     QAALNVNNRVKQAESRLPLEVRRQGVTVEKGSSAFLQVLAFFSPDDRYDDLFTSNYVT
                     LNVLDELKRVPGTTNVQIFGAKDYAMRVWLRPDRLAQLKLTTADVTRALNEQNAQFAA
                     GKVGAAPYGGAQDLVYTITTQGRLADPKQFEDIIVRANADGSTVRLRDVARVELGSKD
                     YEFTGRYNGKRATLMGIFLQPGANALAVAGDVKQTVAELARRFPPGLAYAIPYDTTRF
                     VEVSIREVVKTLGEAMLLVLAVVYLFLQNWRATLIPILAVPVSLIGTFAGLHLLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENIERIMHEEHMNAREAAIEAMREVTGPVIAIVLV
                     LIAVFVPIAFLGGLTGELYRQFSITISIAVGISGLVALTLTPSLCVLILKREHKQPGR
                     FFNAFNRWFGRVRMRYGDGVIWMIRRGAIALVLFGGMVAIAGGLWRITPGSLVPDEDQ
                     GFFIAAVILPDASTLQRTEKVTDEVVQILKSNPVNEDVVAFTGFDFIGGGFRNNAATI
                     FVTQKHWDERTVTTPQLVGEFFMKTAHIKEGLVLAFGPPAIFGLGTAGGYELYLQNRG
                     EGGPQKTAEVLQQL"
     gene            complement(2111..3334)
                     /locus_tag="AC16HB_03855"
     CDS             complement(2111..3334)
                     /locus_tag="AC16HB_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5605955.1"
                     /translation="MNRYRHSLRLPVFAAATLLLAACGGGQQHGGFGGFPPAPVTLME
                     VKTATVPIRFEYVGQTAGSKEAEVRARVQGILEKRTYQEGGAVKAGQQLFVIDPKPYA
                     AQAEQAIAALAQAQAQHAQAKRNLERLQTLLADSAISRREYDDAVSNEESSAAAVKLA
                     QAKLTEAKLNLSYTTVTAPVSGYASRALKSEGSLVTPGQDSLLTTVSQIDPMYVNFSV
                     SENEQLRVNRLLADGKLVAPDGKTGYEVSVRLADGSVFPRKGRLEFIDTRINPATGSY
                     DARALIPNRDAGLKPGQFVRVVVAGATRPSAVLVPQTAVMEGPQGKFVYVAGKSDKGQ
                     DVAQPRPVTVGDWVEVDGANQWIVESGLKPGDKVVIDGMAKIFPVPGGAPIVLGPPPG
                     ADAGKGEMKKSADKK"
     gene            complement(3502..4296)
                     /locus_tag="AC16HB_03860"
     CDS             complement(3502..4296)
                     /locus_tag="AC16HB_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5605956.1"
                     /translation="MNERADVGAQRTPNIAGIGRIYVWNGGSLWIGRAAGRTQMHAHH
                     AIQITLTPDGPVRFCTRSDGEWSEYHGAVIRHDEPHQFDGCGARVAQIFVEPETAAGR
                     ALLARHGDATILRLRDDAADELVRPLFACFAATKAEAPMTAAAQAALDRLAGGTLPSP
                     AIDARLQRALDALGTRLDGVPTLAEVAALAHLSPSRFRHLFVEQMRTPFRSYVLWRRL
                     NIAVESFNRAHNWTDAAHRAGFSDSAHLSRTFRRMFGISPVMLIKE"
BASE COUNT          703 a         1606 c         1467 g          739 t
ORIGIN      
        1 gcagttgctg cagcacctcg gcggtcttct gcggcccgcc ctcgccgcgg ttctgcaggt
       61 acagctcgta gccgcccgcg gtgcccagcc cgaagatcgc cggcggcccg aacgcgagca
      121 ccagcccttc cttgatgtgc gcggtcttca tgaagaactc gccgaccagc tgcggcgtgg
      181 tcaccgtgcg ctcgtcccag tgcttctgcg tgacgaagat cgtcgccgcg ttgttgcgga
      241 acccgccgcc gatgaaatcg aagccggtga acgcgaccac gtcctcgttg accggattcg
      301 acttcaggat ctgcaccact tcgtccgtga ccttctcggt gcgctgcagg gtcgacgcgt
      361 ccggcaggat caccgccgcg atgaagaaac cctggtcctc gtccggcacc agcgagcccg
      421 gcgtgatccg ccacagcccg cccgcgatcg cgaccatgcc gccgaaaagc acgagcgcga
      481 tcgcgcctct gcggatcatc cagatcacgc cgtcgccgta gcgcatgcgc acgcgcccga
      541 accagcggtt gaacgcgttg aagaaccgcc ccggctgctt gtgctcgcgc ttcaggatca
      601 gcacgcacag gctgggcgtg agcgtcagcg cgaccagccc cgagatgccg accgcgatcg
      661 agatcgtgat cgagaactgc cggtacagct cgcccgtcag cccgccgagg aacgcaatcg
      721 gcacgaacac cgcgatcagc accagcacga tcgcgatcac cggccccgtg acctcgcgca
      781 tcgcctcgat cgccgcctcg cgcgcgttca tgtgctcctc gtgcatgatg cgctcgatgt
      841 tctccagcac gacgatcgcg tcgtcgacca cgatgccgat cgccagcacc atgccgaaca
      901 gcgtcagcgt gttgatcgag tagccgagca ggtggagccc ggcgaaggtg ccgatcagcg
      961 acaccggcac cgcgaggatc gggatcagcg tcgcgcgcca gttctgcagg aacaggtaca
     1021 cgaccgcgag caccagcagc atcgcctcgc cgagcgtctt gaccacttcg cggatcgaca
     1081 cctcgacgaa gcgcgtggtg tcgtacggga tcgcataggc gagccccggc gggaagcgcc
     1141 tggcgagttc cgcgaccgtc tgcttgacgt cgcccgcgac cgcgagcgcg ttggcgcccg
     1201 gctgcaggaa gatgcccatc agcgtcgcgc gcttgccgtt gtagcggccg gtgaattcgt
     1261 agtccttcga gccgagctcg acgcgcgcca cgtcgcgcag gcggaccgtc gaaccgtccg
     1321 cgttggcgcg cacgatgatg tcctcgaact gcttcgggtc cgcgagccgc ccctgcgtgg
     1381 tgatcgtgta cacgaggtcc tgcgcgccgc cgtacggcgc ggcgcccacc ttgccggcgg
     1441 cgaactgcgc gttctgctcg ttcagcgcgc gcgtgacgtc cgccgtcgtc agcttcaact
     1501 gcgcgagccg gtcgggccgc agccacacgc gcatcgcgta gtccttggcg ccgaagatct
     1561 gcacgttggt cgtgcccggc acgcgcttca gctcgtcgag cacgttcagc gtcacgtagt
     1621 tggacgtgaa cagatcgtcg taccgatcgt cgggcgagaa gaacgccagc acctgcagga
     1681 aagccgacga gcccttctcg acggtcacgc cctggcgccg cacttccagc ggcaggcgcg
     1741 actcggcctg cttcacgcga ttgttgacgt tcagcgccgc ctggtcgatg tgggtgccga
     1801 tttcgaacgt gacctggatc tgcaccacgc cgttggacgc cgagttcgag ctcatgtaga
     1861 gcatcccttc gacgccgttg atcgcgttct ccagcggcgc ggcgacggtc gattcgagca
     1921 cctgcgcgga ggcgcccgga tacaccgccg ccaccgtcac cacgggcggc gcgatctccg
     1981 ggtactgcgc gatcggcagg atccgcatcg ccgccagccc tgccagcacg atgaagatcg
     2041 acagcaccgc cgcaaatatc gggcgatcga tgaagaagcg cgagaacatg tgagcgtgac
     2101 ggtcggtcgg ctacttcttg tcggcggact tcttcatctc gcccttgccg gcatccgcgc
     2161 ccggcggcgg gccgagcacg atcggcgccc cgcccggcac cgggaagatc ttcgccatgc
     2221 cgtcgatcac gaccttgtcg ccgggcttca gccccgactc gacgatccac tggttcgcgc
     2281 catcgacttc gacccagtcg ccgaccgtca ccgggcgcgg ctgcgccacg tcctgcccct
     2341 tgtccgactt gccggccaca tagacgaact tgccctgcgg cccctccatc accgcggtct
     2401 gcggcaccag caccgcgctc ggccgcgtgg cgcccgcgac gaccacgcgc acgaactgcc
     2461 cgggcttcag tcccgcatcg cgattcggga tcagcgcccg cgcgtcgtac gacccggtgg
     2521 ccggattgat gcgcgtgtcg atgaattcga gccggccctt gcgcgggaac acgctgccgt
     2581 cggccagccg cacgctgacc tcgtagcccg tcttgccgtc cggcgcgacc agcttgccgt
     2641 cggcgagcag gcggttcacg cgcaactgct cgttctccga cacgctgaag ttcacgtaca
     2701 tcgggtcgat ctgcgacacc gtggtcagca ggctgtcctg gccgggcgtg acgaggctgc
     2761 cctcggattt cagcgcgcgg ctcgcgtaac ccgacaccgg cgcggtgacc gtggtgtacg
     2821 agaggttcag cttcgcctcg gtgagcttcg cctgcgcgag cttgaccgct gccgcactgc
     2881 tctcctcgtt cgacaccgcg tcgtcgtatt cgcgcctgct gatcgcgctg tcggccagca
     2941 gcgtttgcag ccgttccagg ttgcgcttgg cctgtgcgtg ctgcgcctgc gcctgcgcga
     3001 gcgcggcaat ggcctgctcg gcctgcgccg catacggctt cggatcgatc acgaagagct
     3061 gctggcccgc cttgaccgcg ccgccttcct ggtaggtgcg cttctcgagg atgccctgca
     3121 cgcgcgcgcg cacctcggcc tccttgctgc ccgcggtctg gccgacgtac tcgaagcgga
     3181 tcggcacggt cgcggtcttc acctccatca gcgtgaccgg cgccggcgga aagccgccga
     3241 agccgccgtg ctgctggccg ccgccgcacg cggcgagcag gagggtcgct gccgcgaaca
     3301 cgggcaaccg caatgaatgg cgatatcggt tcatggcgta aggggtggcg gtcgaagccg
     3361 cacggtgatg tcggccggag agaaggcgcg agcgtaacgg ctcgactgcc gcggcgggtc
     3421 cggcgcgatc gcgaatttac gtcagcggcg ccctgagcac cgaaagctgc tcgttacaga
     3481 tgtaatgagc ctgccgcgcc gctattcctt gatcagcatg acgggcgaga taccgaacat
     3541 gcggcggaac gtgcggctca agtgggccga atcggaaaat cctgcgcggt gtgccgcgtc
     3601 ggtccagttg tgggcccgat tgaacgattc gaccgcgatg ttcagtctcc gccacagcac
     3661 gtagctgcga aacggcgtgc gcatctgctc gacgaacagg tgccgaaaac ggctcggtga
     3721 caggtgcgcc agcgccgcga cctccgccag cgtcggcacg ccgtcgagtc gcgtgcccag
     3781 cgcatcgagc gcgcgctgca ggcgggcgtc gatcgccggt gacggcaaag ttccgccggc
     3841 cagccgatcg agcgccgcct gtgccgcggc agtcatcggc gcttcggcct tcgtcgcggc
     3901 aaagcatgcg aacagcggcc ggacgagttc atcggccgcg tcgtcgcgca accgcaggat
     3961 cgtcgcgtcg ccgtgacgcg cgagcagcgc tcggccggcc gcagtctccg gctcgacaaa
     4021 gatctgcgcc acgcgcgcgc cgcagccatc gaactggtgc ggctcgtcat gtcggatcac
     4081 cgcgccgtgg tactcggacc actcgccgtc gctgcgcgtg caaaagcgca ccggcccgtc
     4141 cggcgtcagc gtgatctgga tcgcgtgatg cgcatgcatc tgcgtccgac cggcagcgcg
     4201 gccgatccac aggctgccgc cgttccagac gtagatgcgg ccgatcccgg cgatgttcgg
     4261 cgtgcgctgc gcgccgacat cagccctttc gttcaagtcg gttcgctcgc cgctacccac
     4321 aatgcgcacc atgccgctcg ccattccggt ggcggaccta aactgaaagg atcgatttat
     4381 ggactcgcaa gcgacttcct gcaactgtgt gaactttccc ggtgccggct gcaactgcgg
     4441 ctgccaggcg caggccggct cgcaggcgtg catctgcggc ccgaactgcg gctgccagaa
     4501 cacgggggcc ggctg
//
LOCUS       DBNHBY010000156         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_617109, whole genome shotgun sequence.
ACCESSION   DBNHBY010000156 DBNHBY010000000
VERSION     DBNHBY010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_617109"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="AC16HB_03865"
     CDS             <1..1542
                     /locus_tag="AC16HB_03865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR5605957.1"
                     /translation="LIVAIADVSRYVQPGDPLDAEAQNRSTSVYFPRRVIPMLPEKLS
                     NGLCSLNPRVDRLVLVADMVITSKGDVSAYQFYPAVMHSHARLTYTEVWEALSVPGSP
                     AAQRLAPLAAQLSDLYDLYRVLAKARDARGAIDFETIETYIVCDANGRIERIVPRTRN
                     DAHKLIEECMLAANVCAADLVARHEHPALYRVHEGPTPDRLANLRALLKSLGLQLDGG
                     DAPQPSDYAALIRKIRARPDVQLLQTVLLRSMQQAIYSPHNAGHFGLAYEHYTHFTSP
                     IRRYPDLLTHRVIKAILARTQFVPQVFGAGAVDDALRARREPKGRAGEALTSWEKLGL
                     LCSANERRADDASRDVEAWLKSYYMRERVGETFSGTINAVVPFGIFVQLDDLFVEGLV
                     HVSELGSEYFSFNDALHELRGERTGIRFRLADRVTVQVARVDLEARRIDFRLVRGTDR
                     KSLQAAAQPPKSTAPPRKGESVEERQARHTARRLRDIDKRERARTGKRGVGGQRSGKL
                     RHRGS"
     gene            1547..2308
                     /gene="rlmB"
                     /locus_tag="AC16HB_03870"
     CDS             1547..2308
                     /gene="rlmB"
                     /locus_tag="AC16HB_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR5605958.1"
                     /translation="MGEDRRTLAGFHAVTARLRHAADSVDAVYFDGRRRDARMQQLVQ
                     RAQAAGVKMLPVDAQRLHELAGDVPHQGVVAVCHASQRARTLDDVLEGMREDTLVLLL
                     DGVTDPRNLGACLRTADAAGAAAVIVPRDRSAPTTAVVAKAAAGAAEAIPLVAVTNLA
                     RAMDDLKQAGCWIVGAVGEADRSLYELDLTGPLAWVLGAEGEGLRRLTRERCDWLARI
                     PMAGHVESLNVSVAAGICLFETQRQRAATGRTGAA"
     gene            2340..2666
                     /locus_tag="AC16HB_03875"
     CDS             2340..2666
                     /locus_tag="AC16HB_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605959.1"
                     /translation="MVAARSSIVFPVLFTRTPLAWLKIGVPGLPPAITLVAIPLLDAL
                     AGARVPKPSARTHPALARWVPCGLALFLLRRQARLHQRPMTRQQRRRPIHRTQQLVRP
                     PQEAAT"
     gene            2666..3403
                     /locus_tag="AC16HB_03880"
     CDS             2666..3403
                     /locus_tag="AC16HB_03880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR5605960.1"
                     /translation="MPDGIVRARQILAQARRVAVLTGAGVSAESGIPTFRDAQTGLWA
                     RFDPMQLASRDGFRADPALVWGWYAARRARMREVEPNPAHRALAAAATRYEQFTLMTQ
                     NVDGLHARAGSRDVIELHGNILRTKCFDACGVVFESPEDLPAGEPPRCPRCRAWLRPD
                     VVWFGEMLDPDALDRAERASAAADAMLVVGTSGLVYPAAGLALTARRHGVPVLIVNPD
                     ESELDSIAEVCVRARAGETLPRILAAD"
BASE COUNT          500 a         1162 c         1246 g          528 t
ORIGIN      
        1 ctgatcgtcg cgatcgccga cgtcagtcgc tacgtgcagc cgggcgatcc gctcgatgcc
       61 gaggcacaga accgctcgac ctcggtctac ttcccgcgcc gcgtgatccc gatgttgccg
      121 gagaagctgt ccaacggtct gtgctcgttg aacccgcgcg tcgaccgact cgtgctggtc
      181 gccgacatgg tgatcacgag caagggcgat gttagtgcct atcaattcta tccggcggtc
      241 atgcattcgc acgcgcggct gacgtacacc gaggtatggg aggcgctatc ggtgcccggg
      301 agtccagcgg cgcagcggtt ggcgccgctc gccgcgcagt tgagcgacct gtacgacctg
      361 tatcgcgtgc tggccaaggc gcgcgacgcg cgcggcgcga tcgacttcga gacgatcgag
      421 acctacatcg tctgcgacgc caacggccgc atcgagcgga tcgtgccgcg cacgcgcaac
      481 gacgcgcaca agctgatcga ggaatgcatg ctcgcggcca acgtgtgcgc ggccgacctc
      541 gtcgcgcgcc acgagcaccc tgcgctgtat cgcgtgcacg agggtccgac gccggaccgg
      601 ctcgcgaacc tgcgcgcgct gctgaagtcg ctcggcctgc aactcgacgg cggcgacgcg
      661 ccgcagccgt cggactatgc agccctgatc cgcaagattc gcgcgcggcc cgacgtgcag
      721 ctattgcaga ccgtgctgct gcgctccatg cagcaggcga tctacagccc gcacaacgcc
      781 gggcacttcg gccttgccta cgagcactac acgcacttca cgtcgccgat tcgccgctac
      841 ccggacctgc tcacgcatcg agtgatcaag gcgatcctcg cgcgcacgca gttcgtgccg
      901 caggtgttcg gcgccggtgc ggtcgacgat gcgctgcggg cgcggcggga gcccaagggg
      961 cgtgctgggg aggcgctcac gagctgggag aagctcggac tgctgtgttc ggccaacgag
     1021 cgccgcgccg acgacgcgtc acgcgacgtc gaagcatggc tgaagagtta ctacatgcgc
     1081 gagcgcgtcg gcgagacgtt ttcgggcacg atcaacgcgg tcgtgccgtt cggcatcttc
     1141 gtgcagctcg acgacctgtt cgtcgagggg ctggtgcacg tgtccgaact gggctcggag
     1201 tacttcagct tcaacgatgc gctgcacgaa ctgcgcggtg agcgtacggg aatccgcttt
     1261 cgactggcgg accgggtgac ggttcaggtc gcgcgcgtcg atctcgaggc gcggcgcatc
     1321 gatttccgcc tcgtgcgcgg caccgatcgc aagtcgctgc aggcggctgc gcagccgccg
     1381 aagtcgacgg cgccgccgcg caaaggcgaa agcgtcgaag agcggcaggc acggcacacg
     1441 gcgcgccggc tgcgcgacat cgacaagcgc gagcgcgcgc gcaccggcaa gcgcggtgtc
     1501 ggcggccagc ggtcgggcaa gctacgccat cgcggcagtt gaggatatgg gcgaggacag
     1561 gcgaacgctg gccgggtttc acgcggtcac cgcgcggctg cgtcacgctg ccgacagcgt
     1621 cgacgccgtg tacttcgacg ggcggcggcg cgacgcgcgc atgcagcaat tggtgcagcg
     1681 ggcgcaggcg gcgggggtca agatgcttcc cgtcgacgcg cagcggctgc atgagctcgc
     1741 gggcgacgtg ccacaccagg gtgtggtcgc ggtctgtcac gcgtcgcaga gggcacgcac
     1801 gctggacgac gtgctggagg gcatgcgcga ggacacgctc gtgctgctgc tcgacggcgt
     1861 gaccgatccg cgcaacctcg gcgcgtgcct gcggacggcg gatgccgccg gggccgctgc
     1921 ggtgatcgtg ccgcgcgatc gttccgctcc gacgacagcg gtcgtcgcca aggcagcggc
     1981 gggtgcggca gaggcgatcc cgctggtcgc ggtcaccaat ctggcgcgcg cgatggacga
     2041 tctgaaacag gcgggctgct ggatcgtcgg cgcggtgggc gaggcggatc ggtcgctgta
     2101 cgaactcgat ctcaccggac cgctcgcgtg ggtgctcggc gcggagggcg agggactgcg
     2161 gcggctgacg cgcgagcgct gcgactggct cgcgcgcatt ccgatggcgg gccacgtcga
     2221 gagcctgaac gtttcggtcg ccgccggcat ctgcctgttc gagacccagc ggcagcgagc
     2281 agcgacgggg cgcaccgggg cggcttgaat ccgacagcgc ttgcgcgcag catgaccgca
     2341 tggtcgctgc gcgttcttca atcgtctttc cggtgctgtt cacgaggacg ccgctcgcgt
     2401 ggctgaagat cggtgtgccg gggttgccgc ctgcgatcac actcgttgcg attccgctgc
     2461 tcgatgcatt ggccggtgcg cgcgtgccga aaccgagtgc gcgcacgcat cccgcgctgg
     2521 cgcgctgggt cccgtgcgga ctggcactgt tcctcctgcg gcggcaggcg cggctccatc
     2581 agcgtccgat gacccgccag cagcgccgcc gcccaatcca ccgaacgcag caactcgtac
     2641 gcccgccgca ggaggcggca acgtgatgcc ggacggcatc gtgcgggcgc ggcagatcct
     2701 cgcgcaggcc cggcgtgtcg cggtgctgac cggcgcaggc gtctcggccg agagcggcat
     2761 tcctacgttc cgcgacgcgc agaccggctt gtgggccagg ttcgatccaa tgcagcttgc
     2821 ctcccgtgac gggtttcgtg ccgatcccgc gctggtgtgg ggttggtacg cagcgcgccg
     2881 cgcgcgcatg cgcgaggtcg agccgaatcc ggcgcaccgc gcgctggcgg ccgcggcgac
     2941 gcgctacgag cagttcacgc tgatgacgca gaacgtcgac ggcttgcacg cgcgcgccgg
     3001 cagccgcgac gtgatcgaac tgcacggcaa tatcctgcgc accaagtgct tcgacgcatg
     3061 cggcgtcgtc ttcgagtcgc ccgaggactt gcccgctggc gagccgccgc gctgcccgcg
     3121 ctgccgcgcc tggctgcgtc ccgacgtggt ctggttcggc gagatgctcg atcccgacgc
     3181 gctcgaccgc gcggagcgcg cgtccgcagc ggcagatgcg atgctggtgg tcggcacttc
     3241 gggcctggtc tatccggcgg cggggctggc gctgacggcg cgccgccatg gcgtgccggt
     3301 gctgatcgtc aaccccgacg aaagcgaact ggactcgatc gccgaggtct gcgtccgcgc
     3361 gcgcgccggc gagacgctgc cgcggatact ggcggccgac tgaattccgg cgccggcccg
     3421 ctttgctccg ggcttg
//
LOCUS       DBNHBY010000157         2880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_685171, whole genome shotgun sequence.
ACCESSION   DBNHBY010000157 DBNHBY010000000
VERSION     DBNHBY010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_685171"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2652)
                     /locus_tag="AC16HB_03885"
     CDS             complement(<1..2652)
                     /locus_tag="AC16HB_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5605961.1"
                     /translation="MAGASAESLVARGWRFAIDRGGTFTDIVGIARDGSLHVDKVLSR
                     DPAHPGDPALRGIGRVLQQQGHRLGRRVESVRLGTTVATNALLERAGAPTLLVVSAGM
                     RDALAIGTQERPDIFARVIRKPSPLYTEVVEVPERTDASGRALTPLDEAALRTVLMAA
                     RERGLRACAIGFLHGFRYPEHERRAAAIARDCGYEEVVASHEAAPLLGFLARTGTAVA
                     DAYLSPVLLRYTREFVASLRQAHGEVAVSFMQSNGGLVDAEGFRGVNGVLSGPAGGVV
                     GMIHAGRSAAGERPLIGFDMGGTSTDVSLAAAGAVPRRYEVELDGIRLRTPVVDVHTI
                     AAGGGSILRLADGRLQVGPASAGADPGPACYRRGGPATVTDCNVVLGRIDTGRFPHVF
                     GPRGDAPLDPAASRRRLGELAQQAQAETGTRYTIEQLAAAYIDVAVTRMAHAIRELAL
                     QRGEDPARCALVCFGGAAGQHACAVADALGIGDVVLHPLAGVLSAYGIALTGRRAIRR
                     RSAECALDATGHGAAARWLEDVAAAARDELLRQGVRPDDCDVHLAAQVRAAGSDVPLD
                     LPWQPLPELLAAFRDAHQHLYGFVDPQASLVIASVVAEASERAPPLPDRALEARPETG
                     LLEATQRTWLDGTWCDVPIRLRSDLRIDDTVTGPLLLCEDGATSWIAPGWTGRIVADG
                     ALRLQRDLQANVQTAEAASAAPDPMRLEVFNALFMHVAEQMGAVLRHTASSVNIKERL
                     DFSCALFDADANLVANAPHMPVHLGSMGASVAAVRDAFRAELRPGDAYVVNSPYAGGT
                     HLPDVTVVAPVFDAAGHHVEFYTASRAHHADIGGITPGSMPPHSRDIGEEGALIEPCR
                     IVRDGVLDEPRVRELLGAGRWPARNLPQ"
     gene            2684..>2880
                     /locus_tag="AC16HB_03890"
     CDS             2684..>2880
                     /locus_tag="AC16HB_03890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR5605962.1"
                     /translation="MRPPRPSRLKKSQPTAKPADDLREAAILAVVRSIPRGRVTTYGQ
                     VAARAGLPRQARLVGRVLSRLP"
BASE COUNT          420 a         1045 c         1010 g          405 t
ORIGIN      
        1 ctgcggcagg ttgcgtgccg gccagcgccc cgcgccgagc agctcccgca cgcgcggctc
       61 gtcgagcact ccgtcgcgca cgatgcggca gggctcgatc agcgcgcctt cctcgcctat
      121 gtcgcggctg tgcggcggca tggacccggg cgtgatgccg ccgatgtccg cgtgatgcgc
      181 gcgcgacgcc gtgtagaact ccacgtgatg gccggcagcg tcgaacaccg gcgccaccac
      241 ggtcacgtcg ggcaggtgcg taccgccggc gtacggcgag ttcacgacat aggcatcgcc
      301 aggccgcagc tcggcacgaa acgcatcgcg gacggccgcc acgctcgcgc ccatcgagcc
      361 gagatgcacg ggcatgtgcg gcgcattggc aaccaggttg gcatcggcgt cgaacagcgc
      421 gcaggaaaaa tcgagacgct ccttgatgtt caccgagctc gccgtgtgtc gcagcacggc
      481 gcccatctgc tcggcgacgt gcatgaacag tgcgttgaac acctcgagcc gcatgggatc
      541 gggtgccgcg ctcgcggctt cggccgtctg tacgttcgct tgcaggtcgc gctgcagccg
      601 taaggcacca tcggcgacga tccgcccggt ccagccgggg gcgatccagc tcgtcgcgcc
      661 gtcctcgcac agcagcagcg gtcccgtgac ggtgtcgtcg attcgcagat cgctgcgcag
      721 gcggatcggc acgtcgcacc atgtaccatc cagccaggtg cgctgcgtcg cctcaaggag
      781 acccgtctcg gggcgcgcct ccagcgccct gtccggcaaa ggcggggcac gctcgctcgc
      841 ctcggcgacg acgcttgcga tgaccagcga tgcctgcggg tcgacgaatc cgtaaaggtg
      901 ctggtgagcg tcgcgaaaag ccgcgagcaa ttcaggcaac ggttgccatg gtaggtcgag
      961 tggcacatcg gaaccggcgg cgcgcacctg cgccgccagg tggacatcgc agtcgtccgg
     1021 ccgcacgcct tgtcgcagca gttcgtctcg cgcagccgcg gcgacgtcct ccagccagcg
     1081 cgcagctgcg ccatggccgg tggcgtcgag cgcgcattcc gcgctgcgtc ggcgtatcgc
     1141 gcgccgtccg gtcagcgcga tgccgtaagc cgacagcacg cccgcgaggg gatgcagcac
     1201 gacgtcgccg atgccgagcg cgtcggcgac ggcgcaggcg tgctgtccgg ccgcgccgcc
     1261 gaagcacacc agcgcgcagc gcgccggatc ttcgccacgc tgcagcgcga gctcgcggat
     1321 cgcgtgtgcc atgcgggtga ccgccacgtc gatgtacgcc gcggcgagct gctcgatcgt
     1381 gtagcgcgtt cccgtctccg cctgtgcctg ctgcgccagt tcgccgagtc gccggcgcga
     1441 cgccgccggg tccagcggcg cgtcgccgcg tggaccgaac acgtgcggga agcgcccggt
     1501 gtcgatacgc ccgaggacca cgttgcagtc cgtcacggtg gccggaccac cacggcgata
     1561 gcacgcgggt cctggatccg cgccggccga tgctggcccc acctgcagcc gaccgtcggc
     1621 gagtcgcagg atcgagccgc cgcctgcggc gatggtgtgc acgtccacga cgggcgtgcg
     1681 caagcggata ccatccagtt cgacctcgta gcgccgtggg acggcgcctg ccgcagcgag
     1741 gctcacgtcg gtcgacgtgc cacccatgtc gaagccgatc agcggtcgct cccccgctgc
     1801 tgagcggccg gcgtggatca tgccgacgac gccgcccgcc ggccccgaca gcacgccgtt
     1861 gactccgcga aagccttccg cgtcgaccag cccgccgttc gactgcatga agctcacggc
     1921 gacctcgccg tgggcctgcc gcaggctcgc gacgaattcg cgcgtgtagc gcagcagcac
     1981 cggcgacagg taagcgtcgg ccacggccgt gccggtgcgc gcaaggaacc cgagcagtgg
     2041 ggctgcctcg tgtgacgcca ccacttcctc gtaaccgcaa tcgcgcgcga tggcggcggc
     2101 gcggcgctcg tgctccggat agcggaagcc gtgcaggaag ccgatcgcgc acgcgcggag
     2161 cccacgttcc ctggccgcca tcagcacggt gcgcaaggcc gcttcgtcga gcggcgtcaa
     2221 ggcgcgccca ctggcgtccg tgcgctcggg cacttcgacg acttccgtgt agagcggaga
     2281 cggcttgcgg atgacccgcg cgaagatgtc gggccgttcc tgcgtgccga tggcgagcgc
     2341 atcgcgcatg cctgccgaga ccaccagcaa ggtcggtgcg ccggcgcgct cgagcaaggc
     2401 attggtcgcc accgtcgtgc cgagccggac cgattcgacg cgccgcccga gacgatgccc
     2461 ctgctgctgc aggacgcgcc cgataccgcg cagggccggg tcgcccgggt gcgccgggtc
     2521 gcgcgacagg accttgtcga cgtgcagtga cccgtcccgg gcgataccga cgatgtcggt
     2581 gaacgtgccg ccgcgatcga tggcgaaccg ccagccgcgc gcaacgagtg actctgcaga
     2641 agctccagcc atggctggct atcgtggcat ccttggtgct gccatgcgac cgcccaggcc
     2701 ctcccgattg aagaagtcgc agcccaccgc gaagccggcg gacgacctgc gcgaggccgc
     2761 catcctcgcc gtcgtgcgtt cgattccgcg tggccgtgtc acgacttacg gacaggttgc
     2821 cgcccgcgcg gggctgccgc ggcaggcgcg gctggtcggc cgcgtgctgt cgcgcctgcc
//
LOCUS       DBNHBY010000158         8650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1023517, whole genome shotgun sequence.
ACCESSION   DBNHBY010000158 DBNHBY010000000
VERSION     DBNHBY010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1023517"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC16HB_03895"
     CDS             <1..322
                     /locus_tag="AC16HB_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5605963.1"
                     /translation="LGARAETFMGLAGVGDLILTCTGELSRNRQVGVLLGRGVPLATA
                     LERIGHVAEGVWSAPAVAARAQSLGVEMPLTAAVCAVLDGQLTPKDALAQLLARDPKR
                     ESAA"
     gene            328..912
                     /locus_tag="AC16HB_03900"
     CDS             328..912
                     /locus_tag="AC16HB_03900"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5605964.1"
                     /translation="MRSACVFCGSQPGARPEYIDVARELGAVFARRGLALIYGGGHVG
                     MMGALADACLAAGGTVTGVIPEHLMRPEVAHQGLTELLVVDSMHTRKRLMAERADAFL
                     VLPGGYGTFEEMFEMITWLQLRLHAKPVGLINVEGYFDHLLAFLRHSAAEGFIRPEHW
                     DLLIVETTPEALLDRLQLHAPAVARGAAADFSKS"
     gene            complement(936..1406)
                     /locus_tag="AC16HB_03905"
     CDS             complement(936..1406)
                     /locus_tag="AC16HB_03905"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR5605965.1"
                     /translation="MFNVILVEPEIPPNTGNVIRLCANTGARLHLVEPLGFDLSDKQL
                     RRAGLDYHECASVRVPSSFDALLDAERCDPQRMFAFTTRGARLLGDVAFLPGDWLVFG
                     AETRGLPERLRERFAREQRIRLPMRAGNRSLNLSNAVAVVVFEAWRQQGYAEGS"
     gene            complement(1409..2110)
                     /locus_tag="AC16HB_03910"
     CDS             complement(1409..2110)
                     /locus_tag="AC16HB_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR5605966.1"
                     /translation="MRLGSTLHRIALGIAPCALCEINDAGADGPLCADCERDFLSPDA
                     SRCAVCAIRLPATAAAICGACLRQPPHFDATLALGDYAPPLDGMVVALKSGGRLALAR
                     VFGELLARRLRSIDGGDALIVAVPLAFERHAERGFNQALEIARCMARMLRLPIDADAL
                     LRMRHAAPQHTLALEERRRNVRDAFSVRADVRGRHLLVVDDVMTTGSTLDEVAAVLKR
                     AGAARVANLVVARTP"
     gene            2153..3088
                     /locus_tag="AC16HB_03915"
     CDS             2153..3088
                     /locus_tag="AC16HB_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5605967.1"
                     /translation="MALGPGTSELTPTIVDPPRVPVRTETVRRQFDRRAAAYPQRDFL
                     AREVERRMLERLDVIRLAPARIVDIGCGYGASRAPLLQRFAGAEWIGIDHSLRMLAAG
                     RRAVGLGRLTRWWRGAREGWIAADAGALPLADGCADFVFSNLMLHWHPTPHRLFPEWK
                     RVLRSDGLLMFSCFGPDTIAELRAAFAAVVPRAMPMPFIDMHDFGDMLVAAGFATPVM
                     DVERIRLTYASPRELLRDVVALGGNPRTDRATGLVSGTRARALLAALQAGRGEDGRIG
                     LTFEISYGHAWNPKPRESGLTRIPVDSLRAQLRAR"
     gene            3139..3615
                     /gene="lysM"
                     /locus_tag="AC16HB_03920"
     CDS             3139..3615
                     /gene="lysM"
                     /locus_tag="AC16HB_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HIR5605968.1"
                     /translation="MGLISFIKEAGEKLFGKEVKAAETGGNIAAANAAAGDAILAYIQ
                     AQNLSATALTVTFDGASGVAKVYGVADDQATREKIILAAGNVQGVAGVEDNMTVSRSG
                     PAAQYYTVVRGDTLSKIAKQFYCDANKYPAIFEANKPMLTHPDKIYPGQNLRIPPL"
     gene            3804..4271
                     /locus_tag="AC16HB_03925"
     CDS             3804..4271
                     /locus_tag="AC16HB_03925"
                     /inference="COORDINATES: protein motif:HMM:NF021517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR5605969.1"
                     /translation="MSEPAIRWMIKRNCSASPVQLATVFVSIVALSFAIGVGFASYGL
                     WLVLPFVGLELIVLASAFVCFGRHAGDYELLELQEGQLHIEQLRGTQRSTWQFEVPRA
                     RLETAASADGSGPGRLFVVAAGERVEIGADLHEERRQRLAHELNRALRSAAAA"
     gene            4321..5460
                     /gene="coxB"
                     /locus_tag="AC16HB_03930"
     CDS             4321..5460
                     /gene="coxB"
                     /locus_tag="AC16HB_03930"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5605970.1"
                     /translation="MDSTRMMTTIRATLAAALLAAAGGAAAIQSRYNLAPPVTQIARD
                     IHWLHWMMLAICGVIFVVVFGVMFYSIYAHRKSKGHKAENFHESTAVEIAWTVVPFII
                     VIVMGALATRTVVAMKDTSNADLTIKATGYQWKWGYDYLKGEGEGISFLSTLATPRDQ
                     IDGGNDAARKANPTYLLEVDNELVVPVDKKVRVITTANDVIHAWFVPALAIKQDAIPG
                     FVRDAWFRAEKTGVYYGQCAELCGKDHAFMPIKVRVVTAEEYTKWVAEKKQAIAALAD
                     DPNKTWTLAELKDRGAKVYAANCQACHQPNGQGIKGTFPALDGSQVVANKEQQIALML
                     AGKGVMPSWKALSDVELAAAATYVRNSWSNNAGPVQPAEFKAARK"
     gene            5511..7103
                     /gene="ctaD"
                     /locus_tag="AC16HB_03935"
     CDS             5511..7103
                     /gene="ctaD"
                     /locus_tag="AC16HB_03935"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR5605971.1"
                     /translation="MSAVVHGHGHAHDAAHEHAPSGWRRWLYATNHKDIGTMYLWFSF
                     TMFLTGGVLALGIRSELFQPGLQIFEPQFFNQLTTMHGLIMVFGAIMPGFVGFANWQI
                     PLQIGAPDMAFARMNNFSFWLLPPAALLLVGSFFVPGGAPAAGWTVYAPLTIQMGMGM
                     DMGIFALHIMGASSIMGSINIITTILNMRAPGMTLMKMPMFVWTWLITAYLLIAVMPV
                     LAGAITMTLTDRHFGTSFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPAFGIVSQIVP
                     VFSRKPLFGYASMVYATASIAILSFIVWAHHMFTTGMPVTGQLFFMYATMLIAVPTGV
                     KIFNWLATMWKGSMTFETPMLWAIGFIFVFTMGGFTGLICAMAPIDIQIQDTYYVVAH
                     FHYVLVAGSLFAMFGGAYFWLPKWTGHMYDEKLGKWHFWLSMIFFNVTFFPMHFLGLA
                     GMPRRYADYPMQFADFNMVASIGAFGFGLSQLVFLWAVLKCIRGGAPAKDNYFDAEDG
                     GLEWTVPSPAPFHTFETPPVVK"
     gene            7108..7269
                     /locus_tag="AC16HB_03940"
     CDS             7108..7269
                     /locus_tag="AC16HB_03940"
                     /inference="COORDINATES: protein motif:HMM:NF038351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HIR5605972.1"
                     /translation="MTPVAARPATQVSMAQQTSEVRQSNNKRTALVLLSIVLVFFIGI
                     IVRRWLFGS"
     gene            7277..7858
                     /locus_tag="AC16HB_03945"
     CDS             7277..7858
                     /locus_tag="AC16HB_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5605973.1"
                     /translation="MAAPGSDTQSNRHTLRKLLVVAVLMFGFGWALIPIYRTICEITG
                     INILTGKDPAAERRAANTQIDRSRTVIVEFDANRQGPWRFRPQVGHLEVHPGELVHVE
                     YDLVNLEDRAMVGQAIPSYAPMQSAKYFNKIECFCFKQQQLAAGETRKFAVVFFVDPE
                     LPKDIGQITLSYTFFEVPGAAPAQAEAARERRS"
     gene            7863..8081
                     /locus_tag="AC16HB_03950"
     CDS             7863..8081
                     /locus_tag="AC16HB_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR5605974.1"
                     /translation="MDAPEPPVVRRKASLGQTARAVFWSFFGVRKRAHYEADAAHLNP
                     VHVIVAGVIGAVLFIAVLVAIIKFVVLR"
     gene            8121..>8650
                     /locus_tag="AC16HB_03955"
     CDS             8121..>8650
                     /locus_tag="AC16HB_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5605975.1"
                     /translation="MGSTHGGAASHYFVPAPARWPMVGMISMIFTTFGAAMLVNRVGG
                     GGWVLLAGFLILLYMMRGWFAEVIGESEGGRYNRQVDVSFRWGMSWFIFSEVMFFASF
                     FGALFYARVITLPSLGDFDHKLLWPDFTPAWPTLGPAGIVGEFSKIHAWPVPTINTIL
                     LVSSGFTLTVAHHGLLS"
BASE COUNT         1400 a         2788 c         2946 g         1516 t
ORIGIN      
        1 gctcggcgcg cgcgccgaga cgttcatggg gctggccggc gtcggcgatc tgatcctgac
       61 ctgcacgggc gagctgtcgc gcaatcggca ggtcggcgtc ctcctcgggc gcggagttcc
      121 gttggccacc gcactcgaac gcatcggcca tgttgccgag ggcgtgtggt cggcgccggc
      181 cgtggccgcg cgcgcgcagt cgcttggcgt ggagatgccg ctgaccgctg cggtgtgcgc
      241 ggtgctcgac ggccagctga cgccgaagga tgcgctggcg caacttctgg cgcgtgatcc
      301 gaagcgggag tccgccgcat gaatccgctg cgctcggcgt gcgtcttctg cggctcgcag
      361 cccggcgcgc ggcccgaata catcgacgtt gcgcgcgaac tcggagcggt gttcgcgcgg
      421 cgcggcctgg cgctcatcta cggcggcggc catgtcggca tgatgggcgc gctcgccgac
      481 gcctgcctgg ccgccggcgg tacggtgacc ggtgtcattc ccgagcacct gatgcggccc
      541 gaggtggcgc atcaagggct gaccgaactg ctggtcgtcg attcgatgca cacgcgcaag
      601 cggttgatgg ccgagcgtgc cgatgcgttt ctcgtgctgc cgggcggcta cggaacgttc
      661 gaagagatgt tcgagatgat cacctggctg cagctgcgtc tgcatgccaa accggtcgga
      721 ctgatcaatg tggagggcta tttcgatcac ctgctcgcgt tcctgcgaca cagcgcggcc
      781 gaagggttca ttcggccgga acactgggac ctgctgatcg tcgagacgac tcctgaggcg
      841 ctgctggaca ggctgcagct gcacgcgccg gcggtggcgc gtggtgccgc ggcggatttc
      901 tcgaagtcgt agcgatcggg cgccgccgca ggctgctacg agccctcggc atagccctgc
      961 tgccgccacg cctcgaacac gactaccgca accgcgttgg aaagattcag gctgcgattg
     1021 ccggcgcgca tcggcagtcg gatccgctgc tcgcgcgcga agcgctcgcg cagtcgctcc
     1081 ggcagaccgc gcgtctcggc gccgaacacc agccagtcac cgggcaggaa cgccacatcg
     1141 ccgagcagcc gcgccccgcg cgtggtgaac gcgaacatcc gctgggggtc acagcgctcg
     1201 gcgtcgagca gcgcatcgaa gctcgaaggc acccgcaccg acgcgcattc gtgatagtcg
     1261 agcccggcgc ggcgcagctg cttgtcgctg agatcgaagc caagcggctc gacgaggtgc
     1321 aaccgcgcgc cggtgttcgc gcacagccgg atcacgttgc cagtgttggg cggtatctcc
     1381 ggttcgacca ggatgacgtt gaacatcgtc agggcgtgcg cgcgaccacg aggttggcca
     1441 cgcgcgcggc gcccgcccgc ttgagcaccg ccgcgacctc gtcgagcgtg ctgccggtgg
     1501 tcatcacgtc gtcgaccacc agaaggtgcc gcccccgcac gtcggcgcgc accgagaatg
     1561 cgtcgcgcac gttgcgtcgc cgttcttcca gtgcgagcgt gtgctggggc gcggcgtgac
     1621 gcatgcgcag cagcgcatcc gcatcgatcg gcaaccgaag catgcgtgcc atgcaccgcg
     1681 cgatctcgag cgcctgattg aatccgcgct cggcatgccg ttcgaacgcc agcggcaccg
     1741 cgacgatcag cgcgtcaccg ccgtcgatcg accgcaagcg tcgcgccagc aactcgccga
     1801 acacgcgcgc cagcgccagc cgaccgccgg acttcagcgc gacgaccatg ccgtcgagcg
     1861 gcggcgcata gtcgccgagc gcgagcgtgg cgtcgaaatg cggcggttgg cgcaggcagg
     1921 cgccgcagat cgccgccgcc gtcgccggca gcctgatcgc gcagaccgcg cagcgcgacg
     1981 cgtcgggcga caggaaatcg cgttcgcagt cggcgcacag cgggccgtcg gcaccggcgt
     2041 cgttgatttc gcacagtgcg cacggcgcga tacctagcgc aatgcgatgc agcgtggagc
     2101 cgagtctcat ccgcgccgag catatactgc ctccgcgcag cagcgccgcc gcatggctct
     2161 cggtcccggt acatccgaac tcactcccac gatcgtcgat ccgccgcgcg ttccggtgcg
     2221 gaccgaaacg gtgcggcgcc agttcgaccg gcgcgccgca gcgtatccgc aacgcgattt
     2281 cctggcccgc gaggtggagc ggcgcatgct cgagcgactc gacgtgatcc ggctcgcgcc
     2341 ggcgcgcatc gtcgatatcg gctgtggcta tggtgcctcg cgcgccccgc tgctgcagcg
     2401 cttcgccggc gccgagtgga tcggcatcga tcattcgctg cgcatgctgg ctgcgggccg
     2461 ccgcgccgtc ggcctggggc gcctcactcg ctggtggcgc ggtgcgcgcg agggatggat
     2521 agccgccgac gcaggcgcac tgccgcttgc cgacggctgc gccgatttcg tgttctccaa
     2581 cctgatgctg cattggcatc cgacaccgca tcgactgttc ccggagtgga aacgcgttct
     2641 gcgcagcgac ggcttgttga tgttcagctg cttcggtccg gacacgatcg cggaactgcg
     2701 cgcagcgttc gccgccgtcg tgccgcgggc gatgccgatg ccgttcatcg acatgcacga
     2761 cttcggcgac atgctggtcg cagccggctt cgcgacgccg gtgatggacg tggagcgcat
     2821 ccgcctgact tatgcctcgc cgcgggaact gctgcgtgac gtcgtcgcgc tcggcggcaa
     2881 tccgcgcacc gaccgggcga ccggactggt ctcgggaacc cgggcacggg cgctccttgc
     2941 cgcattgcag gccggtcgcg gcgaggacgg ccgtatcggg ttgaccttcg agatttccta
     3001 tggtcacgcg tggaacccga agccgcgcga aagcgggttg acgcgcatcc ctgtcgatag
     3061 cctgcgcgcg caattgcgag cgcgttgacg cgcggtcatg ctgcaatacc agaaccgact
     3121 tcaaccagga gagaggacat gggactgatc tcattcatca aggaagcggg cgagaagctg
     3181 ttcggcaagg aggtcaaggc ggcggaaaca gggggcaaca tcgcggccgc caacgcggcg
     3241 gcgggtgacg cgatcctcgc ctacatccag gcgcagaacc tttccgcgac cgcgctgacg
     3301 gtgacgttcg acggcgccag cggcgtggcg aaggtatacg gcgtggccga cgaccaggcg
     3361 acccgggaga agatcattct cgcggccggc aacgtccagg gcgtggcggg ggtcgaggac
     3421 aacatgaccg tctcgcgttc cgggcccgct gcccagtact acacggtcgt ccgtggtgac
     3481 accctgtcga agatcgccaa gcagttctac tgcgacgcga acaagtaccc ggcgatcttc
     3541 gaggcgaaca agccgatgct cacgcatccg gacaagatct atccggggca gaacctgcgc
     3601 ataccgccgc tctgacggca gcagcgcgtt gagggggcgg cctaacgcgg acgttggccc
     3661 gccctcggcg gtccgcatat aatgcgccgc tttgtaattg tcgtggatca aacccattcg
     3721 gttgctaccg agtcagcggc ccgactttcc tcggctgcgc ccgcgtttcc ctgaccgcaa
     3781 gtacgtcgac cagatctcag acgatgtccg agcccgcgat ccgctggatg atcaagcgca
     3841 actgttccgc ctcgccggtg cagctggcaa cggtattcgt gtcgatcgtg gcgctgtcct
     3901 tcgcgatcgg cgtcgggttt gcctcctacg ggttgtggct ggtgctgccg ttcgtgggac
     3961 tcgaactgat cgtgcttgcg tcggcgttcg tctgcttcgg ccgtcacgcg ggggactacg
     4021 agttgctcga actgcaggag gggcaactgc acatcgagca actgcgcggc acgcagcgct
     4081 cgacctggca gttcgaggtg ccgcgcgcgc ggctcgaaac cgcggcgtct gcagacggca
     4141 gcggaccggg ccggctgttc gtcgtggcgg ccggcgagcg ggtggagatc ggcgccgacc
     4201 tgcacgaaga acgccggcag cggctggcgc atgaattgaa tcgcgcgctg cgttcggcgg
     4261 ccgcggcttg acgcatgcag tggaatttgg ggggcggcct gggccgcggc ggatgacttg
     4321 atggattcga ccagaatgat gacaacgatc agggcgacct tggcggcggc attgctcgcg
     4381 gcggcgggcg gagcggcggc catccagagc cgctacaacc tcgcgccgcc ggtgacccag
     4441 atcgcgcgcg acatccattg gttgcactgg atgatgctcg ccatctgcgg cgtcatcttc
     4501 gtcgtcgtgt tcggcgtgat gttctattcg atctacgccc atcgcaaatc gaaggggcac
     4561 aaggccgaga atttccacga gagcacggcg gtcgagatcg cgtggaccgt agtgccgttc
     4621 atcatcgtga tcgtgatggg cgcgctggcc acccggacgg tggtggcgat gaaggacacc
     4681 tcgaatgccg acttgacgat caaggcgacc ggataccagt ggaagtgggg ttacgactac
     4741 ctgaagggcg agggcgaagg catctcgttc ctgtcgacgc tcgccacgcc ccgtgaccag
     4801 atcgacggtg gcaacgacgc ggcgcgtaag gcgaatccga cttacctgct cgaagtcgac
     4861 aacgagctcg tcgttccggt cgacaagaag gtgcgcgtga tcacgaccgc caacgacgtg
     4921 atccacgcgt ggttcgtgcc ggccctcgcc atcaagcagg acgcgatccc gggcttcgtg
     4981 cgcgacgcct ggttccgggc cgagaagacg ggcgtctact acggccagtg cgcggagctg
     5041 tgcggcaagg atcacgcttt catgccgatc aaggtccggg tcgtgactgc agaggagtac
     5101 accaagtggg tcgccgagaa gaagcaggca attgccgcgc tggccgatga cccgaacaag
     5161 acctggacgc tcgccgagtt gaaggacagg ggcgcaaagg tctacgccgc caattgccag
     5221 gcctgtcacc agcccaatgg ccagggcatc aagggaacgt tccccgcgct cgacggcagt
     5281 caggtggtcg ccaacaagga acagcagatc gcgctgatgc tggccggcaa gggcgtgatg
     5341 ccgtcgtgga aggccctgtc cgacgtcgaa ctggcggccg ccgcgactta cgtgcggaat
     5401 agctggagca acaacgccgg gccggtccag ccggcggaat tcaaggcggc gcgcaagtga
     5461 agcgcggcgc cggtacgagc ggaaacggat aacgggaaac aggagccaag atgagcgcag
     5521 tcgtccacgg tcacggtcac gcgcatgacg ccgcccacga gcacgccccg tccggctggc
     5581 gacggtggct ctacgcgacc aaccacaagg acatcggcac gatgtacctg tggttctcgt
     5641 tcacgatgtt cctgaccggt ggcgtgctgg cgctcgggat ccgctcggaa ttgttccagc
     5701 ccgggctgca gatcttcgag ccgcagttct tcaaccagct gaccacgatg cacgggctga
     5761 tcatggtgtt cggcgcgatc atgccgggct tcgtcggctt cgcgaactgg cagattccgc
     5821 tgcagatcgg cgcgcccgac atggcgttcg cgcgcatgaa caacttcagc ttctggctgc
     5881 tgccgccggc cgcgctgctg ctggtcgggt cctttttcgt gcccggcggt gcccccgccg
     5941 ctggctggac cgtttatgcg ccgctgacca tccagatggg catggggatg gacatgggca
     6001 tcttcgcgct gcacatcatg ggcgccagtt cgatcatggg gtcgatcaac atcatcacca
     6061 cgatcctgaa catgcgcgcg cccggcatga cgctgatgaa gatgccgatg ttcgtctgga
     6121 cctggctgat caccgcgtac ctgctgatcg cggtgatgcc cgtgctggcc ggcgcgatca
     6181 cgatgacgct gacggaccgc cacttcggca cgagcttctt caacgccgcg ggcggcggcg
     6241 acccggtgat gttccagcac atcttctggt tcttcggcca tcccgaggtc tacatcatga
     6301 tcttgccggc gttcgggatc gtcagccaga tcgtgcccgt gttctcgcgc aagcccctgt
     6361 tcggttacgc ctcgatggtg tacgcgaccg cgtcgattgc gatcctgtcg ttcatcgtct
     6421 gggcgcacca catgttcacc accggcatgc cggtgaccgg gcaactgttc ttcatgtacg
     6481 cgaccatgct gatcgcagtg ccgaccggcg tgaagatctt caactggctg gcgacgatgt
     6541 ggaagggatc gatgacgttc gagacgccga tgctgtgggc gatcggcttc atcttcgtgt
     6601 tcacgatggg tggcttcacg ggcctgatct gcgcgatggc gccgatcgac atccagatcc
     6661 aggacacgta ttacgtggtg gcgcacttcc actacgtgct ggtcgccggc tcgctgttcg
     6721 cgatgttcgg cggcgcgtac ttctggttgc cgaaatggac cggacacatg tacgacgaga
     6781 agctcggcaa gtggcacttc tggctgtcga tgatcttctt caacgtcact ttcttcccga
     6841 tgcacttcct cgggctggcg ggcatgccgc gtcgctacgc cgattacccg atgcagttcg
     6901 ccgacttcaa tatggtcgcg tcgatcggcg ccttcggctt cgggctgtcg cagctggtct
     6961 ttctttgggc ggtgctgaag tgcatcaggg gcggcgcgcc ggcgaaggac aattacttcg
     7021 acgccgagga cggcggactc gagtggaccg tgccgtcgcc ggcgccgttc catacgttcg
     7081 agacgcctcc cgtggtgaag tagacggatg acgccggtcg ccgcgcggcc agcaacacag
     7141 gtctccatgg cgcagcagac ttcagaagtg cggcagtcga acaacaagcg caccgcgctg
     7201 gtgctgctgt cgatcgtgct ggtgttcttc atcggcatca tcgtgcgccg ctggctgttc
     7261 gggagctgag gatccgatgg ccgcgccggg cagcgacacc caatccaacc gccacacact
     7321 gcgcaagctg ctggtcgtgg ccgtcttgat gttcggcttc ggctgggcgc tgatccccat
     7381 ttaccgcacc atctgcgaga tcaccggcat caacatcctg accggcaagg atcccgcggc
     7441 cgagcggcgc gcggccaaca cgcagatcga ccgctcgcgc accgtcatcg tcgagttcga
     7501 cgccaaccgc cagggtccgt ggcgcttcag gccgcaggtc ggccacctcg aagtgcatcc
     7561 gggcgaactc gtgcatgtcg agtacgacct cgtcaatctc gaggaccgcg cgatggtcgg
     7621 gcaggcgatt ccgagttacg cgccgatgca gtcggcgaag tacttcaaca agatcgagtg
     7681 tttctgcttc aagcagcagc agcttgcggc gggggagacg cgcaagttcg cggtggtgtt
     7741 cttcgtcgat cccgaactgc cgaaggacat cggccagatc acgctgtcgt acacgttctt
     7801 cgaggtgccg ggcgcggcgc ctgcgcaggc cgaggcggcg cgtgagcggc ggagctgaag
     7861 cgatggacgc gccggaaccc cccgtggtcc gccgcaaggc atcgctcggc cagaccgcgc
     7921 gcgccgtgtt ctggtcgttc ttcggcgtgc gcaagcgcgc gcattacgaa gccgatgcgg
     7981 cgcacctgaa tccggtccac gtgatcgtgg cgggggtgat cggcgcggtg ctgttcatcg
     8041 ccgtgctggt ggcgatcatc aagttcgtcg tgctgcgctg aggcggcggg tcaatcgcga
     8101 taaggaagga gaaagggcaa atgggttcga cgcacggggg agcggcatcg cactactttg
     8161 tgccggcgcc ggcgcgctgg ccgatggtgg ggatgatctc gatgatcttc accaccttcg
     8221 gcgcggcgat gttggtcaat cgcgtgggtg gtggcggctg ggtgctgctg gcgggcttcc
     8281 tgatcctgct ctacatgatg cgcggctggt tcgccgaagt gatcggcgag tccgagggcg
     8341 ggcgctacaa ccgccaggtc gacgtgagct tccgctgggg gatgagctgg ttcatcttct
     8401 cggaggtcat gttcttcgcg tcgttcttcg gcgcgctgtt ctatgcgcgc gtgatcacgc
     8461 tgccgtcgct cggcgacttc gaccacaagc tgctgtggcc cgatttcacg ccggcctggc
     8521 ccacgctggg accggccggc atcgtcggcg agttcagcaa gatccacgcc tggccggtgc
     8581 cgacgatcaa cacgatcctg ctcgtgagct cgggcttcac gctgaccgtg gcgcaccacg
     8641 gattgctgtc
//
LOCUS       DBNHBY010000159         2226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_8408, whole genome shotgun sequence.
ACCESSION   DBNHBY010000159 DBNHBY010000000
VERSION     DBNHBY010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_8408"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1433)
                     /locus_tag="AC16HB_03960"
     CDS             complement(63..1433)
                     /locus_tag="AC16HB_03960"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5605976.1"
                     /translation="MNTSTHPSASLAQFAAELRFGDFPEPVVRKTEDLFVDWLGSALA
                     GKGARAVESIARFAEAMGPASGPSEVLIHRRTTSPLLAAAINAASSHVAEQDDVHNGS
                     VFHPATVVFPPALAVAQALGASGAEFLTAAVAGYEVGIRVGEFLGREHYKIFHTTGTA
                     GTLAAAAAVGSLLKLDAEQMLHAFGSAGTQSAGLWEFLRDAADSKQLHTAHASAAGLT
                     AAYLARDGFTGARHILEGPQGMAAGMARDADPTRLVDRLGARWATAETSFKYHASCRH
                     THPAADALLQVMHANALVPAQIARVTAHVHRAALDVLGPVTNPATVHQSKFSMGTVLA
                     LAARFAHAGLPEFEHHFRDADVVDFCARVSMEFDPEVDAAYPARWIGKVTVTTTDGRR
                     LTGRVDDPKGDPGNTLDRAELRAKALRLASFSGAATEPEMNALVDRAWAIAPAQRMPH
                     LLARRD"
     gene            complement(1430..>2226)
                     /locus_tag="AC16HB_03965"
     CDS             complement(1430..>2226)
                     /locus_tag="AC16HB_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5605977.1"
                     /translation="GPYRDKKAYDLLIQSESGFLSITGTPEEPSKAGCSIADIAAGMY
                     GYTNILAALIERGRSGRGVRIDVSMLESMVEWMSYPLYYAFEGASPPPRTGAAHATIY
                     PYGPFRTGDGKTVMLGLQNEREWGEFCAKVLRRPEVATDARFSSNALRVAARKELHAL
                     IVSVFAELTAEQLVARLDEAQIANAQVNSLAAVWEHSQLKARGRWTVVDTPAGPVPAL
                     LPPGVPADFAPRMDAVPALGQHSAAILKELGYAEGEIEGLRTEGAW"
BASE COUNT          338 a          783 c          750 g          355 t
ORIGIN      
        1 ggcgcgatgc aggccagcgc aatcagggca atccatttct tcatcgtgtg tctcctgtca
       61 tttcaatctc tgcgtgcaag caagtgcggc atgcgttgcg ccggcgcgat cgcccaggcg
      121 cgatcgacga gcgcgttcat ctcgggttcg gttgcggcgc cgctgaacga cgccagccgc
      181 agcgccttgg cgcgcagctc ggcacggtcg agcgtgttgc cgggatcgcc cttcggatcg
      241 tcgacgcggc cggtcaggcg ccggccgtcg gtcgtggtca ccgtgacctt gccgatccag
      301 cgcgccggat acgcggcgtc gacctcgggg tcgaactcca tcgacacgcg cgcgcagaaa
      361 tcgacgacat ccgcatcgcg gaagtgatgc tcgaactccg gcagccccgc gtgcgcgaag
      421 cgcgccgcca gcgcgagcac ggtgcccatc gagaacttcg actggtgcac ggtcgccgga
      481 ttcgtcaccg gtccgagcac gtcgagcgcg gcgcggtgca catgcgcggt cacgcgtgca
      541 atctgcgcag gcaccagtgc gttggcgtgc atcacctgca gcaacgcgtc cgcggcggga
      601 tgcgtgtggc ggcacgatgc gtggtacttg aacgaggtct ccgccgtggc ccagcgtgca
      661 ccgagccgat cgacgagccg cgtcggatcg gcgtcgcgcg ccatgccggc cgccatgccc
      721 tgcggtcctt cgaggatgtg ccgcgcaccg gtgaagccat cgcgcgccaa gtacgcggcg
      781 gtcagtccgg ccgcggacgc gtgcgcggtg tgcagttgct tcgaatctgc cgcgtcgcgc
      841 aggaattccc acagccccgc cgactgcgtg cccgccgagc cgaacgcatg cagcatctgc
      901 tcggcatcga gtttcagcag gctgccgacc gccgccgccg cggcgagcgt gcctgcggtg
      961 ccggtcgtat ggaagatctt gtagtgctcg cgaccgagga actcgccgac gcggatgccg
     1021 acctcgtagc cggcgacggc ggccgtcagg aattcggcgc cgctcgcccc gagtgcctgc
     1081 gcaaccgcga gcgccggcgg aaagacgaca gtggccggat ggaacaccga gccgttgtgc
     1141 acgtcgtcct gctcggccac gtgcgacgac gcagcgttga tcgccgccgc cagcagcggg
     1201 ctggtcgtgc ggcgatgaat cagcacttcg ctcgggccgc tcgccggacc catcgcctcg
     1261 gcaaagcgcg cgatcgattc caccgcgcgc gcgcccttgc ccgcgagtgc cgagccgagc
     1321 cagtcgacga acagatcctc ggtcttgcgt acgaccggct caggaaagtc gccgaagcgc
     1381 aactcggccg cgaactgcgc aaggctcgca ctcggatgcg tgctcgtatt caccatgctc
     1441 cttcggttcg gagtccttcg atctcgccct cggcgtaacc cagttccttc aggatcgccg
     1501 ccgaatgctg tccgagcgcc ggcaccgcgt ccatcctggg tgcgaagtcc gccggcacgc
     1561 ccggcggcag cagtgccggc accgggcccg caggcgtgtc gaccacggtc cagcgcccgc
     1621 gcgccttcag ctgagaatgt tcccacacag cggcgagcga attgacctgc gcattggcga
     1681 tctgcgcttc gtcgaggcgc gcgacgagtt gctcggcggt gagttccgcg aacacgctca
     1741 cgatcaacgc atgcagttcc ttgcgcgcgg ccacgcgcag cgcgttggac gagaaacgcg
     1801 cgtcggtcgc gacctcgggc cgtcgcaaaa ccttggcgca gaactcgccc cactcgcgct
     1861 cgttctgcag cccgagcatc accgtcttgc cgtcgccggt ccggaacggt ccgtacggat
     1921 agatcgtcgc gtgcgcggcg ccggtgcgcg gcggcggcga tgcaccctcg aacgcgtagt
     1981 acagcggata gctcatccac tcgaccatgc tctcgagcat cgacacgtcg atgcgcacgc
     2041 cgcgcccgct gcggccgcgc tcgatcagcg cggcgaggat gttggtgtag ccgtacatcc
     2101 cggcggcgat gtcggcgatc gagcagccgg ccttcgacgg ttcttccggc gtgccggtga
     2161 tcgacaggaa gccggattcg ctctggatca gcaggtcgta cgccttcttg tcgcgatagg
     2221 gacccg
//
LOCUS       DBNHBY010000160         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_956305, whole genome shotgun sequence.
ACCESSION   DBNHBY010000160 DBNHBY010000000
VERSION     DBNHBY010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_956305"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="AC16HB_03970"
     CDS             <1..175
                     /locus_tag="AC16HB_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR5605978.1"
                     /translation="RARAHVEEAHRLLTERHAGELVAQELTDAQRQLGEITGEVTSDD
                     LLGRIFASFCIGK"
     gene            292..>1594
                     /locus_tag="AC16HB_03975"
     CDS             292..>1594
                     /locus_tag="AC16HB_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HIR5605979.1"
                     /translation="MKQLSGADAMFLQVERGSNFMHVASLAIYDPSTAPGGSVRFKDV
                     LRFFATRVAQFPQFRRRLVTLPFGIDRPYWLEAASIDVEFHVRHIALPAPGDWRQLCI
                     QVARLHSRPLDRSKPLWEAYVIEGLDNVAGTPPGSFALYTKMHHAIVDGESGTEIMKA
                     LHTLTPEKLDLGDETHDAAAVYHADREPTALELYSRALLNNVERVPAMARLTVSTARR
                     FAALGAESLRTYRREGEGNLITDRLRALLSGDLSALLPGLPQSRFTGKVSAHRVFEAV
                     SFPLADFRKVRKVVPQATINDQFLAIVGGALRAYLSDKGELPEASMIAGVPMTLRGDQ
                     KGGDVGNQVTMTFVAVHTDIEDPIERLRAVCESATKSKRLSDQIGRELIRDVMEALPS
                     LVTDPLLGHTQMPKLGLVVSNVRGPDVPMYMAGAQLVNYVPI"
BASE COUNT          264 a          569 c          519 g          242 t
ORIGIN      
        1 ccgtgcccgg gcccacgtcg aggaagccca ccgcctgctc accgagcgcc acgccggcga
       61 gctggtcgcc caggagctga ccgacgcgca gcgccagctc ggcgagatca ccggcgaagt
      121 caccagcgac gacctgctcg gacggatttt cgccagcttc tgcatcggca aatagcgggg
      181 gtgccccgta cggcaacggc ccggcgcacg gatcagtccg cgggcttctg ggctatcatt
      241 accgacccgg cacgcaacgc ctgccggtgt cattcgcggg gaatcgaggc gatgaaacag
      301 ctgtccggcg cggatgccat gttcctgcag gtggagcgtg gcagcaattt catgcacgtg
      361 gcgtcgctcg ccatctacga cccgtcgacg gcgccgggcg gctcggtgcg cttcaaggac
      421 gtgctgcgat tcttcgcgac gcgcgtcgcg cagttcccgc aattccgccg ccgtctggtc
      481 acactgccgt tcggcatcga ccgtccgtac tggctcgagg cggcgagcat cgacgtcgaa
      541 ttccacgtgc gccacatcgc gctgcccgcg cctggcgact ggcgccagct ctgcatccag
      601 gtcgcgcggc tgcactcgcg cccgctcgat cgcagcaagc cgctgtggga agcgtacgtc
      661 atcgagggcc tcgacaacgt cgcgggcacg ccgcccggca gcttcgcgct gtacaccaag
      721 atgcaccacg ccatcgtcga cggcgaatcg ggcaccgaga tcatgaaggc gctgcacacg
      781 ctgacgcccg aaaagctcga cctcggcgac gagacccatg acgctgccgc ggtctaccac
      841 gccgaccgcg aaccgacggc gctcgagctc tactcgcgcg cgctgctcaa caacgtcgag
      901 cgagtgcccg cgatggcgcg gctcaccgtc agcacggcac gccggtttgc tgcactcggc
      961 gccgagtcgc tgcgcactta ccgtcgcgaa ggcgagggca acctcattac tgaccgcctc
     1021 agggcgctgc tctccggcga cttgagcgcg ctgctgccgg gcctgccgca atcccgcttc
     1081 accggcaagg tctccgcgca ccgcgtgttc gaggccgtgt cattcccgct cgcggacttc
     1141 cgcaaggtcc gcaaggtcgt gccgcaggcg acgatcaacg accagttcct ggcgatcgtc
     1201 ggtggcgcgc tgcgcgccta cttgtccgac aagggcgagc tgccggaggc ctcgatgatc
     1261 gcgggcgtgc cgatgacgct gcgcggcgac cagaagggcg gcgacgtcgg caaccaggtc
     1321 acgatgacgt tcgtcgccgt gcacaccgac atcgaagatc cgatcgagcg cctgcgtgcc
     1381 gtctgcgaat cggccaccaa gagcaagcgg ctcagcgacc agatcgggcg cgagctgatc
     1441 cgcgacgtca tggaagcact gccgtcgctg gtgacggatc cgctgctcgg gcacacccag
     1501 atgcccaagc tgggtctcgt ggtgtccaac gtgcgcggac ccgacgtgcc gatgtacatg
     1561 gcgggcgcac agctcgtgaa ctacgtgccg atca
//
LOCUS       DBNHBY010000161         5129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1023555, whole genome shotgun sequence.
ACCESSION   DBNHBY010000161 DBNHBY010000000
VERSION     DBNHBY010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1023555"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC16HB_03980"
     CDS             complement(<1..853)
                     /locus_tag="AC16HB_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605980.1"
                     /translation="MAGSIPASATGSVAPARGTSPWREAWRRFRRHRLAYASLFVLLA
                     LVAAVLFGPLVWKLPINDIDFAAQLKGPTLAHPFGTDDLGQDLLARMLYGGRISLAVG
                     LAAMTMAVIVGVLVGAIAGSSRGSIDAALMWLTDLFLSLPQLPLLLLIIYLFRDSLKS
                     VLGPEGGVFVLIVAVIGGFRWMPVARLVRAQFFSLREKEFVEAARALGASPTRTVVRH
                     ILPNALGPVIVAGTIDVAAAIIAESTLSFLGLGFPPDIPTWGRILFDSKDHMDIAAHW
                     ALFPGAAI"
     gene            complement(819..1778)
                     /locus_tag="AC16HB_03985"
     CDS             complement(819..1778)
                     /locus_tag="AC16HB_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5605981.1"
                     /translation="MGTYLLRRLLLAVPSLLGISVVLFTVLALAPGDPFGELALNPNV
                     PAEVRMALRAKFGLDDPVWQRYFHWLAAMLQGDWGFSFISRVDVDTLIWQRVPVTLAV
                     IGASQLLALAIALPVGVFSAMRPYSWFDRIASTFAFIGFSLPTFFTGILLILIFSIKL
                     GWLPFVFRADLSTTGWQWWVEMIRQSVMPITVLGLFQGASWTRYVRSAALDVIRLDYV
                     TTARAKGLAERVVVIKHVVRNALIPVVTLVALQMPAVFGGAIVTEQIFRVPGIGSLLI
                     DAILRNDTPVIMAVTFVFSCLVIVFNLIADLLYGWLDPRISYR"
     gene            complement(1782..1943)
                     /locus_tag="AC16HB_03990"
     CDS             complement(1782..1943)
                     /locus_tag="AC16HB_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5605982.1"
                     /translation="MNERDLREAVEAVRAGTLPRRRFVQRLAALGIGAPLAGWDIALA
                     ALHDWYRET"
     gene            complement(2048..3850)
                     /locus_tag="AC16HB_03995"
     CDS             complement(2048..3850)
                     /locus_tag="AC16HB_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5605983.1"
                     /translation="MREQELRDLIEEVREGRLARRAFIRQMVGLGLSAPMASMLLMHA
                     GVASAQTAPPYKPTKRGGGGVLKVLWWQGATLLQPHFANGTKDQEGSRIFYEPLAVWD
                     ADGNLVPILAAEIPTRENGGLAADGKSVTWKLKKGVTWHDGQPFTADDCVFTWEYARN
                     PETAAVTSGVYKDGTVTKVDSHTIRVSFAKPTPFWARAFVAAEGMIIPKHVFGPYIGS
                     KSRDAPGNLKPVGTGPYKFVDFKPGDMVRGELYPNYHMPNRPFFDAIEMKGGGDATSA
                     ARAVLQTGEFDYAWNLQVEDEVLKRLEAGGKGRALIVDSGDVEFIQLNLKDPWNEVDG
                     ERAHMKSKHFAFSDPAVRSAMSLLADRKSMQDFIYGRTGVATSNFLNNPSRYRSPNTK
                     LEFNIDKANQILDAAGWKRGADGIREKDGKKLKFVFQTSVNSLRQKEQAVVKQGCQKA
                     GIDLELKSVTASVFFSSDVANPDTYGKFWCDMQMYTTTMTQPDPERFMDQYLSREVST
                     KANKWQGRNITRWVSEEYDKTYQAVEDELDPVKRAALFIKLNDLPVNDHVIVPLISRP
                     RVRGSGMKLVAPLTGWDLDFSQLQNWYRDGAPTG"
     gene            complement(3945..4916)
                     /locus_tag="AC16HB_04000"
     CDS             complement(3945..4916)
                     /locus_tag="AC16HB_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5605984.1"
                     /translation="METPSTSPHHLRIHRFSALAPGPRLIVTGAVHGNEVAGTRGIAC
                     VLGEIERGEVPIVRGSVTFVPVANPLAYAKGERFGDRNLNRRLLPTSAPRDNEDRIAN
                     VLCPLLAEHDVLLDLHSFRSPGEPFVLRGPVDNDGPLEPFGHAADESRLAAHVGARRV
                     VDGWMEAYAAGVARRRALRLTLDAVNEDPAYGVGTTEYMRSVGGYAVTLECGQHADPA
                     GPEVARQAIRQALALLAIATLPLEPPKGPFECLTLATVVDRHAAGDRFVKDWTSFDPL
                     SAGELIALRADGTEVRAPEPGYIVFPDRAALPGHEWFYLARPSRRPL"
BASE COUNT          856 a         1777 c         1727 g          769 t
ORIGIN      
        1 agatcgccgc gccggggaac agcgcccagt gcgcggcgat gtccatgtgg tccttgctgt
       61 cgaacaggat ccggccccag gtcgggatgt cgggcgggaa tcccagcccg aggaacgaca
      121 gcgtggactc ggcgatgatc gccgccgcca cgtcgatcgt gccggcgacg atcaccggcc
      181 cgagcgcgtt gggcaggatg tgccgcacga cggtgcgcgt gggcgacgcg ccgagcgcgc
      241 gcgcggcctc gacgaactcc ttctcgcgca gcgagaagaa ctgcgcgcgc accagccgcg
      301 ccaccggcat ccagcgaaag ccgccgatca ccgcgacgat cagcacgaac acgccgccct
      361 cgggaccgag cacgctcttc aggctgtcgc ggaacagata gatgatcagc agcagcagcg
      421 gcagctgcgg cagcgacagg aagagatccg tcagccacat cagcgcggcg tcgatcgagc
      481 cgcgcgagct gcccgcgatc gcgccgacca gcacgccgac gatgaccgcc atcgtcatcg
      541 ccgccagccc caccgcgagc gagatgcgcc cgccgtagag catgcgcgcg agcaggtcct
      601 ggccgagatc gtcggtgccg aacggatgcg cgagcgtcgg tcccttcagt tgcgctgcga
      661 agtcgatgtc gttgatcggc agcttccaca ccagcggccc gaacaggacc gccgcaacga
      721 gcgccagcag cacgaacagg ctcgcatacg ccagccggtg gcgccggaag cggcgccacg
      781 cctcgcgcca cggcgacgtg ccgcgcgccg gcgcaacgct accggtagct gatgcgggga
      841 tcgagccagc catacaggag gtcggcgatc aggttgaaca cgatgacgag gcaggagaac
      901 acgaacgtca ccgccatgat gaccggcgtg tcgttgcgca ggatcgcgtc gatcagcagc
      961 gaaccgatgc cgggaacacg gaagatctgc tcggtgacga tcgcgccgcc gaacaccgcc
     1021 ggcatctgca gcgccaccag cgtcaccacc ggaatcaacg cgttgcgcac cacgtgcttg
     1081 atcaccacca cgcgttcggc cagccccttg gcgcgcgcgg tggtcacgta gtcgagccgg
     1141 atcacgtcga gcgcggccga gcgcacgtag cgcgtccagc tcgcgccctg gaacaggccc
     1201 agcacggtga tcggcatgac cgactgccgg atcatctcga cccaccactg ccagccggtg
     1261 gtcgagagat cggcgcggaa cacgaagggc agccagccga gcttgatcga gaagatcagg
     1321 atcagcagga tgccggtgaa gaacgtcggc agcgagaaac cgatgaacgc gaaggtgctg
     1381 gcgatgcggt cgaaccacga gtacggccgc atcgccgaga acacgccgac cggcaacgcg
     1441 atcgccagcg ccagcagttg cgaggcgccg atcaccgcca gcgtgaccgg cacccgctgc
     1501 cagatcagcg tgtcgacgtc gacgcggctg atgaacgaga acccccagtc gccctgcagc
     1561 atcgcggcga gccagtggaa gtagcgctgc cagaccggat cgtcgagccc gaacttggcg
     1621 cgcagcgcca tgcgcacctc ggccggcacg ttgggattga gcgccagttc gccgaacgga
     1681 tcaccgggcg ccagcgccag caccgtgaac agcaccacgc tgatgcccag caagctgggc
     1741 acggcgagca atagccggcg cagcaggtac gtgcccaagg gtcaggtctc gcgataccag
     1801 tcgtgcagcg cagcaagcgc gatgtcccag ccggccagcg gcgcgccgat acccagcgcc
     1861 gccagtcgct gcacgaagcg gcggcgcggc agcgtgcccg cgcgcaccgc ttcgaccgcc
     1921 tcgcgcaggt cgcgctcgtt catccgctcc tctccggaag acgaagcggc atcgtcgctg
     1981 cctcagtgag gcgaccgacg atcagcaggc gccgcagcag ggatgtgccc acggcgtttt
     2041 gcttcgatca gcccgtgggg gcgccgtcgc gataccagtt ctgcaactgc gagaagtcga
     2101 ggtcccaccc ggtgagcggc gccaccagtt tcatgcctga accgcgcacg cgcgggcggc
     2161 tgatcagcgg aacgatcacg tggtcgttga ccggcaggtc gttcagcttg atgaacagcg
     2221 ccgcgcgctt gaccggatcg agttcgtcct cgaccgcctg gtaggtcttg tcgtattcct
     2281 cgctgaccca gcgcgtgatg ttccggccct gccacttgtt ggcctttgtc gagacctcgc
     2341 gcgacaggta ctggtccatg aagcgctcgg gatcgggctg cgtcatggtc gtcgtgtaca
     2401 tctgcatgtc gcaccagaac ttgccgtacg tgtccgggtt ggcgacgtcg gacgaaaaga
     2461 acacgctggc ggtgaccgat ttgagttcca ggtcgatgcc ggccttctgg cagccctgct
     2521 tgacgaccgc ctgctccttc tggcgcagcg agttcaccga ggtctggaac acgaatttca
     2581 gcttcttgcc gtccttctcg cggatgccgt cggcgccgcg cttccagccc gccgcgtcga
     2641 ggatctggtt ggccttgtcg atattgaact cgagcttcgt gttcggcgag cggtagcgcg
     2701 aggggttgtt caggaagttg ctggtcgcca ccccggtgcg gccgtagatg aaatcctgca
     2761 tgctcttgcg atccgccagc agcgacatcg ccgagcgcac cgccgggtcg ctgaacgcga
     2821 aatgcttcga cttcatgtgc gcgcgctcgc cgtcgacctc gttccatgga tccttgaggt
     2881 tcagctggat gaactcgacg tcgccgctgt cgacgatgag cgcgcggccc ttgccgccgg
     2941 cctccaggcg cttcagcacc tcgtcctcga cctgcaggtt ccacgcgtaa tcgaactcgc
     3001 cggtctgcag caccgcgcgc gcggccgagg tggcgtcgcc gccgcccttc atttcgatcg
     3061 cgtcgaagaa cggccggttc ggcatgtggt agttcggata gagctcgccg cgcaccatgt
     3121 cgcccggctt gaagtcgacg aacttgtacg gcccggtgcc gaccggtttc aggttgcccg
     3181 gcgcgtcgcg cgacttcgaa ccgatgtagg gcccgaacac gtgcttcggg atgatcatcc
     3241 cctcggcggc gacgaaggcg cgcgcccaga atggcgtggg cttcgcgaag ctgacgcgga
     3301 tcgtgtgcga gtcgaccttg gtgaccgtcc cgtccttgta gacgccgctg gtgaccgccg
     3361 cggtctccgg attgcgcgcg tactcccacg tgaacacgca gtcgtccgcg gtgaacggct
     3421 ggccgtcgtg ccaggtgacg cccttcttca gcttccacgt gacgctcttg ccgtcggccg
     3481 ccagcccgcc gttctcgcgc gtcgggatct cggccgcgag gatcggcacc aggttgccgt
     3541 cggcgtccca caccgcgagc ggctcgtaga agatgcgcga accttcctga tccttggtgc
     3601 cgttggcgaa gtgcggctgc agcagcgtgg cgccctgcca ccacagcacc ttgagcactc
     3661 cgccgccgcc gcgcttggtc ggcttgtacg gcggcgcggt ctgcgcgctc gcaacgccgg
     3721 cgtgcatcag cagcatcgac gccatcggcg cggagagccc caggccgacc atctgccgga
     3781 tgaaggcgcg gcgcgccagg cgaccttcgc gcacctcttc gatgaggtcg cgcaattcct
     3841 gttcccgcat ggtctgtctc ctcggtattt gtatggaacg ggccggtcat tgtgcaacag
     3901 gccgcacgac gatggaaccg ggcaaagccc gcgtattcag gacgtcatag cggccgccgg
     3961 ctcgggcgcg ccaagtagaa ccactcatgc ccgggcagtg ccgcgcggtc ggggaacacg
     4021 atgtagccgg gctccggggc gcgcacctcg gtgccgtcgg cgcgcagcgc gatcagctca
     4081 cccgccgaca gcgggtcgaa actggtccag tccttgacga agcggtcgcc cgccgcatgg
     4141 cggtcgacga cggtggcgag cgtcaggcat tcgaacggac ccttgggcgg ctccagcggc
     4201 agcgtggcga tcgcgagcag cgcgagcgcc tgccggatcg cttgccgcgc cacttcgggg
     4261 ccggcgggat ccgcgtgctg cccgcactcg agcgtgaccg catagccgcc gaccgagcgc
     4321 atgtactcgg tggtgccgac gccgtaggcc ggatcctcgt tcacggcgtc cagcgtaagc
     4381 cgcagcgcgc ggcggcgcgc cacgccggcg gcataggctt ccatccagcc gtcgacgacg
     4441 cgccgagcgc cgacgtgcgc agccagccgc gattcgtccg ccgcgtggcc aaagggctcg
     4501 agcggcccgt cgttgtcgac cgggccccgc agcacgaacg gctcgccagg actgcgaaag
     4561 ctgtgcagat cgagcagcac gtcgtgctcg gcgagcagcg gacacagcac gttcgcgatg
     4621 cggtcttcgt tgtcgcgcgg cgcgctcgtc ggcagcaggc ggcggttcag attgcgatcg
     4681 ccgaagcgct cgcccttcgc gtaggccagc gggttggcca ccggcacgaa cgtcaccgag
     4741 ccgcgcacga tcggcacctc gccgcgctcg atctcgccga gcacacacgc gatgccgcgc
     4801 gtgccggcga cctcgttgcc gtgcaccgcg ccggtcacga tcagccgcgg accgggcgca
     4861 agcgcgctga agcgatggat gcgcaggtgg tgcggcgaag tcgacggcgt ttccatgcgg
     4921 caaccgtacc ggtgcttcgt ccggcgcgca aggcagtagc atccgcgcat gacgcatgcc
     4981 gcgacttcca tcgatctcac ctgcctgcgc gccgcgctgg cgcggcggat cgcgctgcgc
     5041 accgagagtc aggacccgga acgcaagtcc gaactgcggc gctatctgac cgaggagatc
     5101 gcgccgacgc tgcaacggct gggattcgc
//
LOCUS       DBNHBY010000162         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1362217, whole genome shotgun sequence.
ACCESSION   DBNHBY010000162 DBNHBY010000000
VERSION     DBNHBY010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1362217"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC16HB_04005"
     CDS             <1..515
                     /locus_tag="AC16HB_04005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231681.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HIR5605985.1"
                     /translation="VAMCGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSNPT
                     KLIEVVEIGKQMLMTRGALTTFSISNDVAKYFAIIPAAFASTYPQLNALNVMALASPA
                     SAILSAVIFNALIIVALIPLALKGIKLREDMAAAVLRRNLLIYGVGGLIVPFIGIKAI
                     DLVLVATGLA"
     gene            525..1097
                     /gene="kdpC"
                     /locus_tag="AC16HB_04010"
     CDS             525..1097
                     /gene="kdpC"
                     /locus_tag="AC16HB_04010"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR5605986.1"
                     /translation="MLIDLKRGVILFLLLTVLTGLIYPGVVTGIAHGLLSNQASGSLI
                     VRDGRTVGSALIGQPFSDPKYFWSRPSATGPQPYNGMASGGSNQGPTNRALVDVVRER
                     IAALRSADPRNKGPVPQDLVTASASGLDPHISPQAAEYQLPRVALARGLAEARVRELV
                     GQHTQPRVLGVLGEPRVNVLTLNLALDALR"
     gene            1172..>2904
                     /locus_tag="AC16HB_04015"
     CDS             1172..>2904
                     /locus_tag="AC16HB_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014728.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR5605987.1"
                     /translation="MEERRADPDALLAHVQAEERTAKRGKLKIFFGATAGVGKTYAML
                     EAAQRKKAEGVEVVVGYVEPHGRAETEALLKGLESIPYLMLREGPAQVREFDLDRALA
                     RKPQVLLVDELAHTNASGARHAKRWQDVEELRDAGIDVWTTVNVQHLESLNDVVAGIT
                     SVRMQETVPDTVFESADEVELIDLPPDDLLQRLHEGKVYVPERARVAIENFFRKGNLI
                     ALRELALRKTAERVDAAMDRYRMTKGIAEPWAAGERVMVGVGPTSDGETLVRAGKRLA
                     VALRAPWIVVYVETPELLRLPEAERNRRIAWLRLAETLGAEAVTLGGHAVADELLSYA
                     RTRNVSRLLLGDPSRRGLARWLRPSTVDAVMRRARGVDVTVVKTTETMRIAKSPLLVR
                     SAAYLVPSPSGKLRWPGYVAATGLVAFATAMAWLARSFLNEPNVVMIFLLAIAVAALR
                     FGRGPSVAASAMSVLAYNFFFVPPLFTLNVADGQYLITFAVLMGVGLVIGQLMASVRL
                     QARVAGHRERRTALLYAMTRELTATRGEQNTARVAVRHIGEVFESQVVVLLPDATGRL
                     RYPRDPPEPSSL"
BASE COUNT          457 a          964 c         1005 g          478 t
ORIGIN      
        1 tggtcgcgat gtgcggcgac ggcaccaacg acgcgccggc gctggcgcag gccgacgtcg
       61 ccgtggcgat gaacaccggc acgcaggcgg ccaaggaagc cggcaacatg gtcgacctcg
      121 actcgaaccc gaccaagctg atcgaggtgg tggagatcgg caagcagatg ctgatgaccc
      181 gcggcgcact gacgacgttc tcgatctcca acgacgtcgc caagtacttc gcgatcattc
      241 cggccgcgtt cgcctcgacc tatccgcagc tgaacgcgct gaacgtgatg gcgctggcgt
      301 cgccggcttc ggcgatcctg tcggccgtga tcttcaatgc gctgatcatc gtggcgctga
      361 ttccgctggc actgaagggc atcaagctgc gcgaggacat ggcggccgcg gtgttgcgcc
      421 ggaatctgct gatctacggc gtcggcggat tgatcgtgcc attcatcggc atcaaggcga
      481 tcgacctcgt gctggtcgcc accggattgg cttaggagtc ggacatgctg atcgatctga
      541 aacgcggggt gattctcttc ctgctgctca ccgtcctcac cggactgatc tacccgggcg
      601 tggtgaccgg catcgcacac ggattgctgt cgaaccaggc gagcggcagt ctgatcgtgc
      661 gcgatggcag gacggtgggt tcggccctga tcgggcaacc gttctccgat ccgaagtact
      721 tctggagccg cccgtcggcc accggcccgc agccatacaa cggtatggct tcaggtggat
      781 cgaaccaggg gccgacgaac cgggctctgg tcgatgtcgt ccgggagcgg atcgcggcgc
      841 tgcgctctgc cgatcctcgg aacaagggac cagtcccgca ggatctcgtc accgcttccg
      901 caagtggcct cgatccgcac atcagcccgc aggcagccga ataccagttg ccgcgcgttg
      961 cgcttgcacg cggactggcg gaagcgcggg tgcgcgagct ggtcgggcag catacgcagc
     1021 cgcgcgtact cggcgtgctc ggggagccgc gtgtcaacgt cctgacgctc aacctggcgc
     1081 tcgacgcgct gcgctgaagc agggccggcg attcgtcgtg cggactacga tgccggtgcc
     1141 gccgaagcgt ccgatccact gaccacggac catggaagaa cgccgcgccg atcccgatgc
     1201 attgctcgcg cacgtgcagg ccgaggagcg gactgcaaaa cgcggcaagc tgaagatctt
     1261 cttcggcgcg accgccggcg tcggcaagac ctacgcgatg ctggaggccg cgcagcgcaa
     1321 gaaggccgag ggtgtcgagg tggtcgtcgg ctacgtcgag ccgcatggcc gcgccgagac
     1381 cgaggcactg ctgaaaggcc tggagtcgat tccgtacctg atgctgcgcg aaggcccggc
     1441 gcaggtgcgt gagttcgatc tcgatcgcgc gctggcgcgc aagccgcagg tgctgctggt
     1501 cgatgaactc gcgcacacca acgctagcgg cgcacgccat gccaagcgct ggcaggacgt
     1561 cgaggagttg cgcgacgcgg gcatcgacgt ctggaccacg gtcaatgtcc agcacctcga
     1621 aagcctgaac gacgtcgtcg cgggcatcac ctcggtgcgg atgcaggaga cggtgcccga
     1681 caccgtcttc gagtccgccg acgaggtcga gctgatcgac ctgccgccgg acgacctgct
     1741 gcagcgcttg cacgaaggca aggtctacgt ccccgagcgc gcgcgcgtcg ccatcgagaa
     1801 cttcttccgc aagggcaatc tgatcgcgtt gcgcgagctc gcgctgcgca agacggccga
     1861 gcgcgtcgat gcggcgatgg accgctaccg catgaccaag ggcattgccg agccctgggc
     1921 ggcgggcgaa cgcgtgatgg tcggcgtcgg tccgacgagc gacggcgaga cgctggtgcg
     1981 cgccggcaag cggctggcgg tcgcgctgcg tgcgccctgg atcgtcgtct acgtcgagac
     2041 gccggaactg ctgcgcctgc ccgaggcgga gcgcaaccgg cgcatcgcct ggctgcggct
     2101 cgccgagacg ctcggcgccg aggcggtgac gctcggtggc catgccgtcg ccgacgagct
     2161 gctgagctac gcacgcacgc gcaacgtttc gcgcctgctg ctcggcgatc cgagccggcg
     2221 cggcctggcg cgctggctgc gaccgtcgac cgtcgatgcc gtgatgcgcc gggcccgcgg
     2281 tgtcgatgtc acggtcgtga agacgaccga aaccatgcgg atcgcgaagt ctccgttgct
     2341 cgtgcgcagc gccgcctatc tcgtgccctc gccgtcgggc aagctgcgct ggccgggcta
     2401 cgtggccgcg acgggtctcg tcgcctttgc caccgcgatg gcgtggttgg cgcggtcctt
     2461 cctcaacgaa cccaacgtgg taatgatctt tctgctggcg atcgcagtcg cggcgctgcg
     2521 cttcgggcgt gggccctccg tcgccgcctc ggcgatgagc gtgctggcct ataacttctt
     2581 cttcgtgcct ccgctgttca cgctgaacgt cgccgacggc cagtatctga ttacctttgc
     2641 cgtgctgatg ggagtcggcc tcgtgattgg tcagctgatg gcgagcgtgc ggctgcaggc
     2701 acgcgtcgcg ggccaccgcg agcgtcgcac cgcgctgctg tacgcgatga cgcgagagct
     2761 gacggccacg cgcggcgagc agaacaccgc ccgcgtcgcc gtgcgccaca tcggcgaagt
     2821 gttcgagtcc caggtcgtcg ttctgctgcc ggacgcgacc ggacgcctgc ggtatccacg
     2881 cgacccgccg gagccgtcat cgct
//
LOCUS       DBNHBY010000163         2822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1023643, whole genome shotgun sequence.
ACCESSION   DBNHBY010000163 DBNHBY010000000
VERSION     DBNHBY010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1023643"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC16HB_04020"
     CDS             complement(<1..962)
                     /locus_tag="AC16HB_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042884 - microcin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HIR5605988.1"
                     /translation="MLAYILKRILLMVPTLIGALTVTFVVIQFVPGGPVETLLAEARI
                     GAGGAYKASRDVDRQQIEELKKLYGFDKPVHVRYVEMLESFARFDLGRSFLRNKDVWD
                     LIKEKLPVSVSLGVWTFLISYLISIPLGVSKAVREGSRFDAATTMAVLVGYAIPGFVL
                     GVLLIVVFAGGTFLDWFPLRGLVSDNWAELSWPRRIADYFWHLTLPLICLTIGSFATI
                     TMLTKNTFVEEIRKQYVLVARAKGLSQDRVLYKHIFRNALIPLVTGFPAAFVGAFFTG
                     ALLIETLFSLDGLGLLSYESVIRRDYPVVLGSLYLFTLIGLIVKL"
     gene            complement(988..>2822)
                     /locus_tag="AC16HB_04025"
     CDS             complement(988..>2822)
                     /locus_tag="AC16HB_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5605989.1"
                     /translation="CLLLAVPALAPAADGQWVHAYAAFGEPKYPRGFTHFEYANPDAP
                     KGGVLNLRNPDRRSSFDKFNYFTVRGNAPAGVGIFMLESLATLSADEPRTMYGLLAEQ
                     MLIAPDRSSITFRLDPRARFSNGDPVLAVDVKYSFDMQTGKYASPIYQTPLAGVEKAE
                     VLDERTIRFGLRDRSSDTLFKVGLMMVFSHQWGLKPDGTHTRFDEIVSEYPIASGPYT
                     IALADSGRRIEFRRNPDYWARDLPVRRGFYNFDRVVYRYYQDEAVATEAFKAGEFDLV
                     RVYSARIWVRQHKGRKWDDGRIVKQAFPVATGQGLQSYQLNLRRPIFQDIRVREALNW
                     SYDYDTNNRYRLYKRASSVFNNSEFAAQGLPSPGELKLLEPFRRELPPQVFGPPFVAP
                     GTGGDPAKLRQNLLRARALLEAAGWKLDAQGVLRNAQGEPFEFEYLAPGDSTGREEAW
                     QRNLDKLGIRLKTRNVDFALYNRRLEEYDFDMVTIVEGAFTLPSAADYVTYYGSKAAD
                     EKGNNNYRGVKSAAVDHILDAMNRATTMDEFRDACRALDRVVTWNWWQVPELYSDFEA
                     ISYWNKFGMPAVRAKYFTADLAPDVDPQLPWPLATWWIKAPEQR"
BASE COUNT          497 a          924 c          930 g          471 t
ORIGIN      
        1 agcttgacga tgagcccgat cagcgtgaac aggtacagcg acccgagcac caccggatag
       61 tcgcgccgga tcaccgattc gtacgacagg aggcccaggc cgtcgagcga gaacagcgtc
      121 tcgatcagca gcgcaccggt gaagaaggca ccgacgaaag cggccgggaa gccggtgacg
      181 agcgggatca gcgcgttgcg gaagatgtgc ttgtacagca cgcggtcctg cgacagaccc
      241 ttggcgcgcg cgaccagcac gtactgcttg cggatctctt cgacgaatgt gttcttggtc
      301 agcatcgtga tcgtcgcgaa gctgccgatg gtcaggcaga tcagcggcag cgtcaggtgc
      361 cagaagtagt cggcgatgcg ccgcggccac gacagttcgg cccagttgtc ggaaaccagc
      421 ccgcgcagcg gaaaccagtc gagaaacgtg ccgccggcga acaccacgat cagcagcacg
      481 ccgagcacga agcccggtat cgcatagccg accagcaccg ccatcgtggt ggcggcgtcg
      541 aagcgcgagc cctcgcgcac tgccttggac acgccgagcg ggatcgagat caggtacgaa
      601 atcaggaagg tccagacgcc gagcgagacc gacaccggca gcttttcctt gatcaggtcc
      661 cacacgtcct tgttgcgcag aaagctgcgc ccgagatcga agcgcgcgaa ggattcgagc
      721 atttcgacgt agcgcacgtg caccggcttg tcgaagccgt acagcttctt cagctcctcg
      781 atctgctggc ggtcgacgtc gcggctcgcc ttgtacgcgc cgccggcgcc gatgcgcgct
      841 tcggccagca gcgtttcgac cgggccaccg ggcacgaact ggatcacgac gaacgtgacc
      901 gtcagcgcgc cgatcagtgt cggcaccatc agcaggatgc gcttgaggat gtaggcgagc
      961 atcagccgtc cgccgtcgcg cgaaccgtca tcgctgctcc ggcgccttga tccaccacgt
     1021 cgccagcggc cacggcaact gcggatcgac gtcgggcgcg aggtcggcgg tgaagtattt
     1081 cgcgcgcacc gccggcatgc cgaacttgtt ccagtacgag atcgcctcga aatcggaata
     1141 cagttccggc acctgccacc agttccacgt cacgacgcgg tcgagcgcgc ggcaggcgtc
     1201 gcggaactca tccatcgtcg tggcgcggtt catcgcgtcc aggatgtggt ccaccgccgc
     1261 gctcttgacg ccgcgatagt tgttgttgcc cttctcgtcg gcggccttgc tgccatagta
     1321 ggtcacgtag tccgcggctg acggcagcgt gaacgcgcct tcgacgatcg tgaccatgtc
     1381 gaagtcgtac tcctccagcc ggcggttata cagcgcgaag tcgacattgc gcgtcttcag
     1441 ccggatgccg agcttgtcga gattgcgctg ccacgcttcc tcgcggccgg tcgagtcgcc
     1501 gggcgcgagg tactcgaact cgaacggttc gccctgcgca ttgcgcagca cgccctgcgc
     1561 gtccagtttc catccggccg cctccagcag cgcgcgcgcg cgcagcaggt tctgtcgcag
     1621 cttggccgga tcgccaccgg tgccgggcgc aacgaagggc gggccgaaca cctgcggcgg
     1681 caattcgcgg cggaacggct cgagcagctt caactcgccc ggggacggca gcccctgcgc
     1741 cgcgaactcg gaattgttga atacgctgct cgcacgcttg tacagccggt agcggttgtt
     1801 ggtgtcgtag tcgtacgacc agttgagcgc ctcgcgcacg cggatgtcct ggaagatcgg
     1861 ccggcgcagg ttgagctggt aggactgcag cccctgcccg gtcgcaaccg ggaacgcctg
     1921 cttgacgatg cgaccgtcgt cccatttccg gcccttgtgc tgacgcaccc agatgcgcgc
     1981 gctgtacacg cgcacgaggt cgaactcacc ggccttgaat gcctcggtcg cgaccgcctc
     2041 gtcctggtaa tagcggtaga cgacgcgatc gaagttgtag aagccgcgcc gcaccggcag
     2101 gtcgcgcgcc cagtaatccg gattgcgcct gaactcgatg cggcgtcccg agtcggccag
     2161 cgcgatcgtg tagggcccgc tcgcgatcgg gtactcgctc acgatctcgt cgaagcgcgt
     2221 gtgcgtgccg tccggcttca gcccccactg gtgcgagaag accatcatca gtccgacctt
     2281 gaacagcgtg tcgctgctgc ggtcgcgcag gccgaagcgg atcgtgcgct cgtcgagaac
     2341 ttccgccttt tccacccctg ccagtggcgt ctggtagatc ggcgatgcgt acttgccggt
     2401 ctgcatgtcg aagctgtact tgacgtccac ggccagcacc gggtcgccgt tggagaagcg
     2461 cgcgcgcgga tcgagacgga acgtgatcga cgacctgtcg ggcgcgatca gcatctgctc
     2521 ggcgagcaga ccgtacatcg tgcgcggctc gtcggccgac agcgtcgcca gggactccag
     2581 catgaagatg ccgacgccgg ccggcgcgtt gcccctgaca gtgaagtagt tgaacttgtc
     2641 gaaactggag cggcgatccg ggttgcgcag gttcagcacg ccgcccttgg gcgcatcggg
     2701 attcgcgtac tcgaagtggg tgaagccgcg cgggtacttc ggctcgccga aggccgcgta
     2761 ggcgtgtacc cattggccgt cggcggcagg cgcgagtgcc ggcaccgcga gcagcaggca
     2821 aa
//
LOCUS       DBNHBY010000164         4005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1700768, whole genome shotgun sequence.
ACCESSION   DBNHBY010000164 DBNHBY010000000
VERSION     DBNHBY010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1700768"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="AC16HB_04030"
     CDS             complement(<1..798)
                     /locus_tag="AC16HB_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5605990.1"
                     /translation="MQCSLATLLSFALSPVAFAQPAPTATGETTLAPVVVTATRVERP
                     LFDVPASISVIDRDTLRDAQLRVNLSESLGRVPGLVVLNRENYAQDLQISIRGFGSRS
                     TFGVRGVRLYVDGVPASFPDGQGQVSHFPLDAAERIEVLRGPFSALYGNSSGGVIALT
                     TELKPQPFRAELNGAAGENSTWRIGANAVGGTDPYAFAIDATRFDTNGTRPHSAARRD
                     TLNLRWAALESPLGRVRLALNSVSMPDAQDALGLTRAQFDADPDQTAA"
     gene            complement(839..1168)
                     /locus_tag="AC16HB_04035"
     CDS             complement(839..1168)
                     /locus_tag="AC16HB_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5605991.1"
                     /translation="MLLRLIAAAALAAASAAAPACAQNIAAGARKAASCQACHGLDGI
                     AKLPDAPNLAAQPAPYLERALRAYRSGERRNEVMSIAARSLSDEDVRDLAAYYAAIQI
                     EIRALPK"
     gene            complement(1173..2444)
                     /locus_tag="AC16HB_04040"
     CDS             complement(1173..2444)
                     /locus_tag="AC16HB_04040"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5605992.1"
                     /translation="MKVRPSAVALAVLLVIAGAATAQQNIEKLKQMKVSGTDLNIPTV
                     PQTGKNADAIRENLKRIRLPAGFKIDLYAIVPDARHMAVAPSTNMLFVGTRKTTVWAV
                     TDRNGDYVADEVKPFAPSLNFKVPNGVCWTKDGFLIVVEHNRVLNFPAAEFFYEGPDV
                     AVIEVVPQGKLVPEDEESYNHGARTCRIGSDGMLYITLGQPHNVQPAGKVALYEKLGI
                     GGIVRMNPFDGSKREVFAVGIRNSVGQDFNPKDGTLWFTDNQTDGLGDDVPPGELNRA
                     TKPGQHFGYPYWNGHFKVAGSPAAPDLKDLKEPANAVYPQIEFPAHQAQLGMTFYAGK
                     MFPAKYQGGIFVASHGSWNRTKASGALINFVSLKADGTADKSEVFAEGWLDGETGTYR
                     GRPVDVAVMKDGSLLVSDDYAGAIYRITYQP"
     gene            complement(2636..3070)
                     /locus_tag="AC16HB_04045"
     CDS             complement(2636..3070)
                     /locus_tag="AC16HB_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5605993.1"
                     /translation="MVSRVLVATDGSPRSQKAIEVAVGIAKALNAALVGCTATPPYPY
                     YGLGEVLPDSEGQYRTAASAAATERLTEVERAARAAGVVCTTVIKEQQHPHLAILQTA
                     QEADCDLIVMASRGRGEVSALFIGSETQRVLAFADRPVLVVR"
     gene            complement(3102..3518)
                     /locus_tag="AC16HB_04050"
     CDS             complement(3102..3518)
                     /locus_tag="AC16HB_04050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR5605994.1"
                     /translation="MSTIHVDVVSAEESIFAGEAEFVVLPGEAGELGIYPRHAPLITR
                     IKPGVVRIKVPARSDEEQVFVAGGILEVQPTVITVLADTAIRGKDLDEAKANEALKAA
                     EEARKNATSELEIAKVEAEMSALAAQLAAIKKLRGK"
     gene            complement(3540..>4005)
                     /locus_tag="AC16HB_04055"
     CDS             complement(3540..>4005)
                     /locus_tag="AC16HB_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase beta subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR5605995.1"
                     /translation="SPATTFGHLDATVVLSRDIAALGIYPAVDPLDSTSRQVDPNTIG
                     EEHYNTTRRVQATLQRYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVAE
                     VFTGSPGKYVPLKETIRGFKMIVEGECDALPEQAFYMVGTIDEVFEKAKKLQ"
BASE COUNT          657 a         1330 c         1286 g          732 t
ORIGIN      
        1 tgcagcggtc tggtccggat ccgcatcgaa ctgcgcgcgc gtcagcccga gtgcatcctg
       61 cgcgtcgggc atcgataccg aattcaacgc gaggcgaacc cgcccgagtg gtgattcgag
      121 cgcggcccag cgcaggttca gcgtgtcacg ccgcgccgcg ctgtgcggac gcgtgccatt
      181 ggtgtcgaac cgggttgcat caattgcgaa cgcataggga tcggtgccgc cgaccgcgtt
      241 ggcgccgatg cgccaggtgg aattctcgcc cgcagcgccg ttcagttcgg cgcgaaaggg
      301 ctgcggcttg agttcggtcg tcaacgcgat cacgccgccc gacgaattgc cgtaaagcgc
      361 cgagaacggg ccgcgcagaa cctcgattcg ctccgcggcg tcgagcggga agtgggacac
      421 ctgcccctgg ccatcgggaa acgaggcggg cacgccatcc acgtacagcc tcacgccgcg
      481 cacgccgaac gtcgagcgcg acccgaagcc gcggatcgag atctgcagat cctgcgcgta
      541 gttctcgcgg ttcagcacga cgagccccgg cacgcggccg agcgactcgg acagattgac
      601 gcgcagttgc gcgtcgcgca gcgtgtcgcg atcgatcacg gagatcgacg cgggaacgtc
      661 gaacagcggg cgctcgacgc gggtcgcggt caccacgacc ggcgccagcg ttgtttcccc
      721 agttgcggtc ggcgctggct gcgcgaacgc cacaggcgac agcgcgaatg acaacaaggt
      781 cgcgagactg cattgcattc gagatgcctg caccgggaga cgaaagccgc agcccagact
      841 atttcggcag cgcccggatc tcgatctgga tcgctgcata gtacgcagcc aggtcgcgca
      901 cgtcttcgtc gctcagcgat cttgcagcga tcgacatcac ctcgttgcgc cgctcacccg
      961 atcggtatgc acgcagcgcg cgttcaagat agggcgcggg ctgggcagcg aggttcggcg
     1021 catccggcag tttggctatt ccgtcgagtc cgtggcacgc ctggcacgag gccgccttgc
     1081 gtgccccggc cgcgatgttc tgcgcgcacg ccggagctgc ggcagacgcc gccgccaacg
     1141 cggcggcggc gatcaggcgc agcaacatca catcacggct gatacgtgat gcggtagatc
     1201 gcacccgcgt aatcgtccga gacgagcagc gatccgtcct tcatcaccgc cacatcgacc
     1261 ggacgcccgc gataagtacc ggtttcacca tcgagccaac cttcggcgaa tacttctgac
     1321 ttgtcggccg tgccgtccgc cttcaacgat acgaaattga tcagcgcgcc gcttgccttg
     1381 gtgcggttcc acgagccgtg cgacgcgacg aagatgccgc cctggtattt cgccgggaac
     1441 atcttgcctg cgtagaaagt catgccgagc tgtgcctgat gggcggggaa ctcgatctgc
     1501 ggatacaccg cgttcgccgg ctccttgagg tctttcaagt ccggcgccgc gggcgagcca
     1561 gccaccttga agtgtccgtt ccaatacggg tacccgaaat gctggcccgg cttcgtggca
     1621 cggttcagtt cgcccggcgg aacgtcgtcg cccaggccgt ccgtctggtt atcggtgaac
     1681 caaagcgttc cgtccttcgg gttgaaatcc tgaccgacgg agtttcgtat gccgaccgcg
     1741 aagacctcgc gcttggaccc atcgaacgga ttcatacgca cgatcccgcc gatgccgagc
     1801 ttctcgtaca gcgcaacctt gcctgccggt tgcacgttgt gcggttgccc gagcgtgatg
     1861 tagagcatgc cgtcggatcc gatgcggcag gtgcgcgcgc cgtggttgta tgactcctcg
     1921 tcttccggaa ccagcttgcc ctgcggcacg acctcgatca cggcgacatc cggtccttca
     1981 tagaagaact cggcggccgg gaaattcagg acgcggttgt gctcgacaac gatcaggaat
     2041 ccatctttgg tccagcacac tccgttgggc accttgaagt tcagcgaggg ggcgaatggc
     2101 ttgacttcat ccgccacgta gtcgccgttg cgatcggtta ccgcccacac cgtggtcttg
     2161 cgggtgccga cgaacagcat gttggtactc ggggcaacgg ccatgtggcg cgcgtcgggc
     2221 acgatggcgt agaggtcgat cttgaagcca gccggcagtc tgatgcgttt gagattctcg
     2281 cggatcgcat ctgcattctt ccccgtctgc ggcacggttg gaatgttcag gtccgtgccc
     2341 gagaccttca tctgcttgag cttctctatg ttctgctgcg ccgtggcggc gccggcaatc
     2401 acaagcagga ccgccagcgc aactgcgctt ggtctcacct tcatgtctgt ctcctgagcg
     2461 tcgattgttg tgctgtccgc gcgcgaacgc ggcgcgccga atatacggtc cacccactgc
     2521 ctcgtccacg cgacggccaa ttcgaggact cgcgcaagca cgcgaggtag aaggcaagag
     2581 cgggtgacgt acgagactgg cgcgaaacct gcgccgcgct cactggcgcg gcggctcacc
     2641 gcacgacgag cacgggccga tcggcgaacg ccaataccct ctgcgtctcg ctgccgatga
     2701 acagcgcgga gacttcgccg cgaccgcgcg atgccatcac gatcaggtcg cagtcggctt
     2761 cctgcgcggt ttgcaggatg gccagatgcg ggtgctgctg ttccttgatg acggtcgtgc
     2821 agaccacacc ggcggcgcgc gccgcgcgct cgacctccgt caggcgctcg gtcgccgcag
     2881 cactcgccgc cgtgcggtac tggccctcgc tgtcgggcag gacctccccc aatccatagt
     2941 atggatacgg gggcgttgcc gtgcatccga ccagtgctgc attcagcgcc tttgcgatgc
     3001 cgacggcgac ctcaattgcc ttttgcgatc tcggcgaacc gtcggtggcg accagaactc
     3061 gcgagaccat gtcggcggac tccgttcagg gcaacggcgg gctacttgcc gcgcagcttc
     3121 ttgatcgccg ccagctgggc agcgagcgcc gacatctcgg cctcgacctt ggcgatttcg
     3181 agttcgctgg tggcgttctt gcgcgcttct tcggccgctt tcagcgcctc gttggccttg
     3241 gcctcgtcga gatccttgcc gcgaatcgcg gtgtcggcca gcacggtgat caccgtcggc
     3301 tgcacttcga gaatgccgcc ggcgacgaac acctgctcct cgtcgctgcg agccggcacc
     3361 ttgatgcgca ccacgccggg cttgatgcgc gtaatcagcg gcgcatggcg tggatagatg
     3421 ccgagctcgc cggcttcgcc cggcagcacg acgaactcgg cctcgccggc gaagatgctc
     3481 tcctcggcgc tgacgacgtc gacatggatg gttgacatga gtcgctttcc cgccgcgcgc
     3541 tactgcagct tcttcgcctt ctcgaacacc tcgtcgatcg tgccgaccat gtagaacgcc
     3601 tgctccggca gcgcgtcgca ttcgccctcg acgatcatct tgaagccgcg gatcgtctcc
     3661 ttcagcggca cgtacttgcc gggcgacccg gtgaacacct cggcgacgtg gaacggctgc
     3721 gacaggaagc gctggatctt gcgcgcgcgc gccaccgcca gcttgtcctc gggcgacagc
     3781 tcgtccatgc cgaggatcgc gatgatgtcg cgcagctcct tgtagcgctg cagcgtggcc
     3841 tgcacgcggc gcgtcgtgtt gtagtgctcc tcgccgatcg tgttggggtc gacctggcgc
     3901 gacgtcgaat cgagcgggtc caccgccgga taaatgccga gcgcggcgat gtcgcgggag
     3961 agcaccacgg tcgcgtccag atggccgaac gtcgtcgcgg gcgac
//
LOCUS       DBNHBY010000165         2614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1768250, whole genome shotgun sequence.
ACCESSION   DBNHBY010000165 DBNHBY010000000
VERSION     DBNHBY010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1768250"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16HB_04060"
     CDS             <1..200
                     /locus_tag="AC16HB_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5605996.1"
                     /translation="PDAAKKGKPGTPEFRAGLRDAIEATHDLVASQGVINLSAQDHSG
                     FDQRGQALITVRDGKFVLVKD"
     gene            220..>2614
                     /locus_tag="AC16HB_04065"
     CDS             220..>2614
                     /locus_tag="AC16HB_04065"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5605997.1"
                     /translation="MSVAAVTLEDKYRLERGRVFMTGVQALARLPMLQAARDHAAGLN
                     TAGYVSGYRGSPLAGLDNALHDARAHLAAHGVAFHPAVNEELAATAIWGTQQLHLLPG
                     ARRDGIFALWYAKGPGVDRCGDVFKHANHAGTSKHGGVLLVAGDDHSARSSAVAHQSE
                     HIFSACGIPVLAPADLQDCLDYGLHGWAMSRYCGCWIAMKTTSDIVESSATVELDPQR
                     VESVVPDDFALPPDGVHIRWPDPQLAQERRMQEFKVYAAVAYARANRLNRLVWDAPRP
                     RVGIIACGKAWLDVRQALHDLGIDAQVARDIGLRLFKVGMAWPLEADGVRHFAEGLEE
                     ILVVEEKRQIIEYQLKEMLYNWREDVRPRVVGKFDESGEWPAPHGQWLLPPTGELTPA
                     LAARAIAARIGRFHSSESMRRHLAFLDERERAPAGVPARAPWFCSGCPHNRSTRVPEG
                     SCALGGVGCHLMATWMDRGTLTVSQMGGEGVTWLGMAEHAGAPHVFANMGDGTYYHSG
                     LLAIRAAVAAGVNITYKVLYNDAVAMTGGQPVDGPLTVPQITRQLAAEGVQRIVVLSE
                     ETGKYTDLAPGVAVRARDELDAAQRELRTVPGVSALIFDQTCAAEKRRRRKRGALPAA
                     PRRVFIHELVCEGCGDCSLKSNCLSVVPVQTEFGRKYAIDQASCNVDESCLAGLCPSL
                     VTIEGGTPRRAQPVQAEGEPPPDPGLPALDVPYSLLVAGIGGTGVVTVAALVGMAAHL
                     DGHGVTVLDMTGLAQKGGAVLSHVRIARTQEELAAPRIASGAAAALLGCDALVAASAD
                     "
BASE COUNT          386 a          920 c          947 g          361 t
ORIGIN      
        1 tcccggacgc ggcgaagaag ggcaagcccg gcacgcccga gttccgcgcc gggctgcgcg
       61 acgcgatcga ggccacgcat gacctggtcg catcgcaggg cgtgatcaac ctgtcggcgc
      121 aggaccactc cggcttcgac cagcgcggcc aggcgctgat caccgtgcgc gacgggaagt
      181 tcgtgctggt gaaagactga gtcgtttacg tcagcggcca tgagcgtcgc ggccgtcacg
      241 ctcgaagaca agtaccgcct cgagcgcggc cgcgtgttca tgacgggcgt gcaggcgctc
      301 gcgcgcctgc cgatgctgca ggcggcgcgc gatcacgccg cggggctgaa taccgcaggc
      361 tacgtgtccg gctaccgcgg ctcgccgctg gcggggcttg acaacgcgct gcacgacgcg
      421 cgcgcgcatc tcgccgcaca cggcgtcgca ttccatccgg cggtcaacga ggaactggcc
      481 gccaccgcga tctggggcac gcagcagttg cacctgctgc cgggcgcgcg gcgggacggc
      541 atcttcgcgc tgtggtacgc gaagggaccc ggcgtcgatc gttgcggcga cgtgttcaag
      601 cacgccaacc acgccggcac gtcgaagcac ggcggcgtgc tgctcgtcgc cggcgacgat
      661 cattccgcgc gctcgtcggc ggtcgcgcac cagagcgagc atatcttctc cgcctgcggc
      721 atcccggtgc tcgcacccgc cgacctgcag gattgcctcg actacggcct gcacggctgg
      781 gcgatgtcgc gctactgcgg ctgctggatc gcgatgaaga cgacctcgga catcgtcgag
      841 agctcggcca cggtcgagct cgatccgcag cgcgtcgagt ccgtcgtgcc ggacgatttc
      901 gcgctgccgc ccgacggcgt gcacatccgc tggcccgacc cgcagctcgc gcaggagcgg
      961 cggatgcagg agttcaaggt ctacgcggcg gtggcgtatg cgcgcgcgaa ccggctgaac
     1021 cggctcgtgt gggacgcgcc gcggccgcgg gtcggcatca tcgcctgcgg caaggcgtgg
     1081 ctcgacgtgc ggcaggcgct gcacgacctc ggcatcgacg cgcaggtcgc gcgcgacatc
     1141 gggctgcgcc tcttcaaggt cggcatggcg tggccgctcg aggccgacgg cgtgcggcat
     1201 ttcgccgaag ggctcgagga gatcctggtc gtcgaggaaa agcgccagat catcgaatac
     1261 cagctcaagg aaatgctcta caactggcgc gaggacgtgc gcccgcgcgt cgtcggaaaa
     1321 ttcgacgaaa gcggcgaatg gccggcgccg cacgggcagt ggctgctgcc gccgaccggc
     1381 gagctgacgc cggcgctcgc ggcgcgcgcg attgcggcgc gcatcgggcg ctttcactcc
     1441 tcggagtcga tgcgccggca cctcgcgttc ctcgacgaac gcgagcgcgc gcccgccggc
     1501 gtgccggcac gtgcgccgtg gttctgctcg ggctgtccgc acaaccgctc cacgcgcgtg
     1561 ccggaaggat cgtgcgcgct cggcggcgtc ggttgccacc tgatggcgac ctggatggat
     1621 cgcggcacgc tcacggtgag ccagatgggc ggcgaaggcg tgacctggct cggcatggcc
     1681 gagcatgcgg gcgccccgca cgtattcgcc aacatgggcg acggcaccta ttaccactcc
     1741 ggtctgctcg cgatccgcgc cgcggtcgcc gccggggtca acatcacgta caaggtcctg
     1801 tacaacgatg cggtcgcgat gacgggcggg caaccggtcg acgggccgct gacggtgccg
     1861 cagatcacgc gccagcttgc cgccgagggc gtgcagcgca tcgtcgtgct gagcgaagaa
     1921 accggcaagt acacggacct cgcacccggc gtcgcggtcc gtgcccgcga cgaactcgat
     1981 gccgcgcagc gcgaactgcg cacggttccc ggcgtcagcg cgctgatctt cgaccagacc
     2041 tgcgcggccg agaagcgccg gcgccgcaag cgcggcgcgc tgcccgcggc gccgcgccgt
     2101 gtgttcatcc acgagctcgt atgcgagggc tgcggcgact gcagcctgaa gtcgaactgc
     2161 ctgtcggtgg tgccggtgca gaccgagttc ggccgcaagt acgcgatcga ccaggcgagc
     2221 tgcaacgtcg acgagagctg cctcgcggga ctgtgcccga gcctggtcac gatcgaaggc
     2281 gggacgccgc ggcgcgcgca gcccgtgcaa gccgaaggcg agccgccgcc cgaccccggc
     2341 ctgcccgcgc tcgacgtgcc gtacagcctg ctcgtcgccg gcatcggcgg caccggcgtg
     2401 gtgacggtgg ccgcgctggt cggcatggcc gcgcacctcg acgggcacgg cgtcaccgtg
     2461 ctcgacatga ccgggctggc acagaagggc ggcgccgtgc tgtcgcacgt gcgcattgcg
     2521 cgcacgcagg aagaactcgc cgccccgcgc atcgccagcg gcgccgccgc cgcgctgctt
     2581 ggttgcgacg cgctggtggc cgcgagtgcc gatg
//
LOCUS       DBNHBY010000166         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_211705, whole genome shotgun sequence.
ACCESSION   DBNHBY010000166 DBNHBY010000000
VERSION     DBNHBY010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_211705"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1043
                     /locus_tag="AC16HB_04070"
     CDS             18..1043
                     /locus_tag="AC16HB_04070"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR5605998.1"
                     /translation="MSDQSDLQATVDALVQKGKGILAADESSPTIAKRFKAIGVESTE
                     ENRRAYRQLLLATPGIGEFISGVILFEETLGQRADDGTPLPQLAARQGIVPGIKVDAG
                     KIPLAHAPGDEITQGLDGLAKRLAGYKPLGARFAKWRAVYNVSPTLPSRLAIEANAEA
                     LARYAAICQEQGFVPIVEPEVLIDGNHTMERCAEVTEAVLHEVFHALHRHGVVLECTL
                     LKPSMVVPGKEHPQPAKPAEVAAWTIRILKRTVPAAVPGIFFLSGGQTPAEASANLDA
                     INRLGTQPWQLSFSYGRALQDPAMKAWKGQAANARETQNALLKRARLNGAAREGRYLP
                     ALEDARD"
     gene            complement(1201..1575)
                     /locus_tag="AC16HB_04075"
     CDS             complement(1201..1575)
                     /locus_tag="AC16HB_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5605999.1"
                     /translation="MRAAFDRFVQIQNAHDAKALETLLVDSPQFLWITRGTVVWGREA
                     ALQRFAKLYEGTWRLDPDPSSVRVTPISDNAAQLHAAVQFTTGAPNQPPQVTRTLLNQ
                     VLVKSDGTWRVMSIFPIPAPAQ"
     gene            1798..>2154
                     /locus_tag="AC16HB_04080"
     CDS             1798..>2154
                     /locus_tag="AC16HB_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5606000.1"
                     /translation="MKGHEKPVYRFGPFLLLPSERMLLHDGEPVALAGKAFDLLVALV
                     SQAGHLLTKDELLRRVWPGLVVEEVNLSVNMSAIRKALACAPDTADWIETVPRQGYRF
                     KAPVEVEDVATLKQVWP"
BASE COUNT          423 a          674 c          705 g          352 t
ORIGIN      
        1 gcaaggagga acggatcatg agtgatcaaa gcgatcttca agccacggtt gacgcactgg
       61 tgcagaaggg caagggaatc cttgctgccg acgaaagcag ccccacgatc gcaaagcggt
      121 tcaaggcgat cggcgtcgag tcgacggagg agaaccgccg cgcctatcgc cagctcttgc
      181 ttgcaacgcc cggcatcggc gaattcatca gcggcgtcat cctcttcgag gagacgctgg
      241 gccaacgggc ggacgatggc acgccgttgc cacaactcgc ggcacgccag ggcatcgtgc
      301 ccggcatcaa ggtcgatgcc ggcaagatcc cgctggcgca cgcacccggt gatgagatca
      361 cgcagggcct ggatggactt gcgaagcgtc tggccggcta caagccgctg ggcgcgcgct
      421 ttgcaaagtg gcgcgctgtc tacaacgtat cgcctacgct tcccagccgg ctggcgatcg
      481 aggccaacgc agaagcgttg gcgcgctatg ccgccatctg ccaggaacaa ggcttcgtcc
      541 cgattgttga acccgaggtc ctgatcgacg gtaaccacac catggagcgc tgtgccgagg
      601 tcaccgaggc agttctgcac gaagtgttcc acgcgctgca tcgacacggg gtcgttctcg
      661 aatgcacgct gctgaaaccg agcatggtcg tgcccggcaa ggagcacccg caaccggcga
      721 agcctgcgga agtcgccgca tggacgatcc ggatcctgaa gcgcacggta ccggctgcgg
      781 tgcccggaat cttctttctg tcgggcgggc agacaccggc agaggcgagc gcgaatctcg
      841 acgcgatcaa ccgactcggc acgcaaccgt ggcagttgag cttctcgtat ggacgcgcgc
      901 tgcaggatcc ggccatgaaa gcctggaaag ggcaagctgc caatgcacgc gagacacaga
      961 acgctctgct caagcgcgcc cggctcaacg gcgccgcgcg cgaaggaaga tacctaccgg
     1021 cgctggagga cgctcgggat tgagtcgacg ttgagcgcga gacgcgcgga ctcttcgaca
     1081 agggccgtcg aaatgaatgc ggcgcgaatc gctgcaggca cttgaagtgc tggcgggccc
     1141 ggcagcattc gaacccacga ccccttggtt cgtacgtcct gcatcccggc gactcgcacc
     1201 tcactgcgcg ggcgccggaa ttggaaagat gctcatcacc cgccaggtgc cgtctgactt
     1261 gacgagcacc tggttcagca gggtgcgtgt cacctgcgga ggctggtttg gcgcgccggt
     1321 cgtgaactgc accgccgcat ggagctgcgc ggcgttgtcc gaaatcggcg tcactcgtac
     1381 gctgctcggg tccgggtcga gccgccaggt gccttcgtag agtttcgcaa agcgctgcaa
     1441 cgccgcctcg cggccccaga ccacggtgcc gcgtgtgatc cagaggaact gtggcgaatc
     1501 gacaagcagc gtttccagcg cctttgcatc gtgcgcgttc tgtatctgca caaagcggtc
     1561 gaaagcggcg cgcacctcat cctcggtcga cgcgaggaca cctccgactg aacacgtgag
     1621 tgcgatggca gtgctgagcg cgcagaaggc gcggcgaggg gacagggaca tgacagactc
     1681 ctccgacaca ggtataccgg cggcccgccg gtactgagaa gattcgaacc aggctcgcct
     1741 aaagtccctg cgaaactcct gtttttcgcc tgcaaatcgc ctatggaggc atagccgatg
     1801 aaaggccacg aaaagcccgt gtatcgcttc ggcccgttcc tgctgttgcc gagcgagcgc
     1861 atgctgctgc acgatggcga gccggttgcg ctcgccggca aggcgttcga cttgctcgtg
     1921 gcgctcgtga gccaggcagg ccatctgctg acaaaggacg agttactacg acgcgtttgg
     1981 ccaggccttg tcgtcgagga ggtcaacctc agcgtcaaca tgtcggccat ccgcaaggca
     2041 cttgcctgcg cgccggacac ggccgactgg atcgaaacgg tgccgcggca gggctaccgg
     2101 ttcaaggcgc cagtcgaggt ggaggacgtc gcgaccttga agcaggtctg gccg
//
LOCUS       DBNHBY010000167         2889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_414688, whole genome shotgun sequence.
ACCESSION   DBNHBY010000167 DBNHBY010000000
VERSION     DBNHBY010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_414688"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="AC16HB_04085"
     CDS             complement(<1..878)
                     /locus_tag="AC16HB_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HIR5606001.1"
                     /translation="MKRRQLLQASGALALGASGITAAFGQSKTPIKVGILHSLSGTMA
                     ISETALKETALMTIAEINAKGGVMGRPLEPVVVDPASNWPLFAEKARQLITQDKVAVV
                     FGCWTSVSRKSVLPVFKELNHLLFYPVQYEGEELEKNVVYTGAAPNQQAIPATEYLMS
                     KEGGAAKRFFLLGTDYVYPRTTNKILRAFLKSKGVAEKDIEEIYTPFGHSDYQTIVAN
                     VKKFSGGGKTCVISTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPL
                     VGHLAAWNYFMSLKNP"
     gene            complement(1097..1498)
                     /locus_tag="AC16HB_04090"
     CDS             complement(1097..1498)
                     /locus_tag="AC16HB_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606002.1"
                     /translation="MTNPAQAGSAPQAIDDDSIPVLTERLTLPALDLDFALPTLERVI
                     EAEPPAPPAPPPAPPPPTVDELRDAVLRAVLERLPAEIEQLVREQLRAAIDGAIAHLA
                     TEANQAIRAALSEIIERAVREALSERGDASG"
     gene            complement(1495..1920)
                     /locus_tag="AC16HB_04095"
     CDS             complement(1495..1920)
                     /locus_tag="AC16HB_04095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR5606003.1"
                     /translation="MRVDFYFNVDHRLHYACRVVRKARTSNLSVLVYARDADRLARFD
                     AALWTFSALDFVPHVYADSPLAAQTPVWLTLAPSGDARRDLLLNLDDDVPATDWCTRF
                     ARVVEVVSKDDQDRAHARTRFRAWRERGVTPVAHEVGEE"
     gene            complement(1957..>2889)
                     /locus_tag="AC16HB_04100"
     CDS             complement(1957..>2889)
                     /locus_tag="AC16HB_04100"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR5606004.1"
                     /translation="TKRLGNLPANVCTPTYLGEQARQLGNEWKLDVEVLGRKDIEKLK
                     MGSFLSVTRGSAEPPRLIVARYHGAAKSQAPVVLVGKGITFDSGGISLKPGAHMDEMK
                     FDMCGAASVLGTLRAVAEMKLKVNVIGVIAACENLPSGTATKPGDIVTSMSGQTIEVL
                     NTDAEGRLILCDALTFVERFKPAAVIDVATLTGACVVALGDINSGLFATDDALADELL
                     KAARVAIDPAWRMPVEEDYQEQLKSNFADMANIGAPGKAGAVIAACFLARFTKAYKWA
                     HLDVAGTAWKTGSAKGATGRPVPLLANFLLQRAP"
BASE COUNT          441 a         1005 c          939 g          504 t
ORIGIN      
        1 ttcggattct tcagcgacat gaagtagttc cacgccgcca gatggcccac cagcggcttg
       61 gtgtcgacgc cgcgcagttc ctcctcgccg accgagaacg cgaccaccgg cacgtccttc
      121 gctttcaggc ccgcgttgcc caactccttg tagaaaggca cgttcgaatc gccgttgatg
      181 gtcgagatca cgcacgtctt gccgccgccg gagaacttct tcacgttggc cacgatcgtc
      241 tggtaatcgc tgtgaccgaa gggcgtgtag atctcctcga tgtccttctc ggccaccccc
      301 ttgctcttga ggaacgcgcg caggatcttg ttggtcgtgc gcgggtacac gtagtcggtg
      361 ccgagcagga agaagcgctt ggccgcgccg ccttccttgc tcatcaggta ctcggtggcg
      421 gggatcgcct gctggttggg cgccgcaccc gtgtagacga cgttcttctc cagctcctcg
      481 ccctcatact gcaccgggta gaagagcagg tggttcagct ccttgaacac cggcagcacg
      541 ctcttgcgcg acacgctcgt ccagcagccg aagacgaccg cgaccttgtc ctgcgtgatc
      601 agctggcgtg ccttctccgc gaacagcggc cagttcgacg cggggtcgac gaccaccggc
      661 tccagcggcc gccccatgac gccgcccttg gcgttgatct cggcgatcgt catcagcgcg
      721 gtttccttca gcgccgtttc cgagatcgcc atcgttcccg acagcgaatg caggatgccg
      781 accttgatcg gcgtcttcga ctgcccgaag gcggccgtga tgccgctggc gccgagtgcc
      841 agcgcgcccg aggcttgcaa caactgtctg cgtttcattc cgaagttccc ccaccggttg
      901 gacgtacgcg aggattcgcg tcaacgaaag gggaacaaga tccgtgccgg gccgagaaag
      961 ccgtcgcgcc gcgcgtattg cacaacaccg gtgctggtat gcaccacttg ggcaagtttt
     1021 ggaacacatc tgcaccacgg acagccatgc tggcttgccc ggcgcagccg gcgcgctgcg
     1081 gcgatctgtg gaacgctcaa cccgacgcgt cgccgcgttc gctcaaggcc tcgcgtaccg
     1141 cacgttcaat gatctcgctc agcgcggcgc ggattgcctg atttgcttcc gtcgccaggt
     1201 gcgcgatcgc gccgtcgatc gcggcacgca gctgctcgcg caccagttgc tcgatctccg
     1261 ccggcaagcg ttcgagcacg gcccgcagca cggcgtcgcg cagctcgtcc accgtcggcg
     1321 gtggcggggc cggcggcggc gccggcggcg cgggcggctc ggcttcgatc acccgctcca
     1381 gcgtcggcag cgcgaagtcc aggtcgagcg cgggcagcgt gaggcgctcg gtcaggaccg
     1441 gaatcgaatc gtcgtcgatc gcctgcggcg ccgagccagc ctgcgcggga ttcgtcattc
     1501 ctcgcccact tcgtgcgcga ccggcgtgac gccgcgctcg cgccacgcgc ggaagcgggt
     1561 ccgcgcgtgc gcgcgatcct ggtcgtcctt cgacacgact tcgaccacgc gcgcaaagcg
     1621 cgtgcaccaa tccgtcgcgg gcacgtcgtc gtcgaggttc agcagcaggt cgcgccgcgc
     1681 atcgccgctc ggcgccagcg tcagccacac cggcgtctgc gctgcgagcg gtgaatccgc
     1741 gtacacgtgc ggaacgaagt cgagcgccga gaaggtccat agcgctgcat cgaagcgcgc
     1801 cagtcggtcc gcgtcgcgcg cgtagaccag caccgacagg ttcgacgtgc gtgccttgcg
     1861 caccacgcgg caggcgtagt gcagccggtg atcgacgttg aagtagaagt cgacgcgcat
     1921 cgcgcgtcca tcatacggcg gcgcggtgcg atcccatcac ggcgcgcgct gcaacaggaa
     1981 attggccagc agcggcaccg gccggccggt cgcgcccttt gcgctgccgg tcttccacgc
     2041 ggtgccggcg acgtcgaggt gggcccattt gtacgccttg gtaaagcggg cgagaaagca
     2101 ggcggcgatc acggcaccgg ccttgccggg tgccccgatg ttggccatgt ccgcgaagtt
     2161 cgacttcaac tgctcctggt agtcctcctc gaccggcatg cgccacgccg ggtcgatcgc
     2221 gacgcgcgcc gctttgagca gttcgtcggc gagcgcgtcg tcggtggcga acaggcccga
     2281 gttgatgtcg cccagcgcga ccacgcaggc gccggtcagc gtggcgacgt cgatcaccgc
     2341 cgccggcttg aagcgctcca cgaaggtcag cgcatcgcac aggatcaggc gcccctcggc
     2401 atcggtgttc agcacctcga ttgtctggcc cgacatcgag gtcacgatgt cgccgggctt
     2461 ggtcgccgtg cccgacggca ggttctcgca cgcggcgatc acgccgatca cgttgacctt
     2521 cagcttcatc tcggcgaccg cgcgcagcgt gccgagcacc gaggccgcac cgcacatgtc
     2581 gaatttcatc tcatccatgt gggcgccggg cttcagcgag atgccgccgc tgtcgaacgt
     2641 gatgcccttg ccgaccagca cgaccggcgc ctgcgacttc gccgcgccgt ggtacctggc
     2701 cacgatcagg cgcggcggct cggccgagcc gcgcgtgacc gacaggaacg aacccatctt
     2761 cagtttctcg atgtccttgc ggcccagcac ttcgacgtcg agcttccatt cgttgccgag
     2821 ttgccgcgcc tgctcgccga ggtaggtcgg cgtgcacacg ttggcgggca ggttgcccag
     2881 gcgcttggt
//
LOCUS       DBNHBY010000168         3440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1023954, whole genome shotgun sequence.
ACCESSION   DBNHBY010000168 DBNHBY010000000
VERSION     DBNHBY010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1023954"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16HB_04105"
     CDS             complement(<1..470)
                     /locus_tag="AC16HB_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606005.1"
                     /translation="MDQNLPNPVRPAFDKPISDDHVIVNIGPSHPATHGTVQIIAELD
                     GERVVRTDIHPGYLHRGFEKECEDHTWHNLIPYVDRLNYCSALINDFAYCDAVERLMG
                     VEITPRCRYLRTLLAEYSRIADHMTCTAAALMELGAMTAFLYLVTIRDAIYEHLA"
     gene            complement(474..938)
                     /locus_tag="AC16HB_04110"
     CDS             complement(474..938)
                     /locus_tag="AC16HB_04110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5606006.1"
                     /translation="MDVFARVKERLPAAQAADVSHGTLVVFIAPDAVLATAQKLKSEF
                     DFDLFTDVTAVDWLGQAPRFEVVWHFYSTRHFARIRVKARVDESDPTVDSLVSLYGSA
                     RYMERECHDMYGIRFRGNPDLRPVLLYEGFQGHPLRKDYPKQQEQPLVPYRS"
     gene            complement(941..1459)
                     /gene="nuoB"
                     /locus_tag="AC16HB_04115"
     CDS             complement(941..1459)
                     /gene="nuoB"
                     /locus_tag="AC16HB_04115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HIR5606007.1"
                     /translation="MNAKSIPIVPAQGGKSAAFEYITTKKDELAGWARKFSLFPYPFV
                     TACCAMEFMAVSGTPYDTDRFGAALPRFSPRQADLLMVVGTVTHKQAPILLKVYEQMC
                     EPKWVMAFGVCASSGGFYQNYAAVAGIDKIIPVDVFVPGCPPRPEMVIDGIMQLQDKI
                     ARGKHPIITREF"
     gene            complement(1459..1824)
                     /locus_tag="AC16HB_04120"
     CDS             complement(1459..1824)
                     /locus_tag="AC16HB_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5606008.1"
                     /translation="MSPYAVFLLYMVAILGFVALTLLLNKLLGPKPAMTASKLEPFEC
                     GATPVETQNVKAVPAKYYAVAIVFILFDLETVFLFIWALGAQPMTGFMLATFAIFMAL
                     LVLILLYVYKARLLEAVTE"
     gene            complement(2059..>3440)
                     /locus_tag="AC16HB_04125"
     CDS             complement(2059..>3440)
                     /locus_tag="AC16HB_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR5606009.1"
                     /translation="GASAGHFGRRRHMHGQATHAATAIFGLNPLWIAVALFVATYVLI
                     MTEKVNRAILALLGAGLLILLGVVTQGEAMGSVDFNTLGLLLGMMLIVNITRRSGLFQ
                     FVAIWSAKKVDARPWGILLMMSIVTAVFSAFLDNVTTVLLTVPVTLLITDELKVKPYP
                     YLFAQIFASNIGGTATLIGDPPNIMIGSATGLTFNDFAIALTPVIIVVLVATLVPLWY
                     IWGKHLHADAEHRQRVLGFNEREAITDVRLLKQSLTVIALVIVGFMLQRQIRIEPATI
                     AIFGAAALLLLDNLGRDPEKQSHNVHAALSEAEWITLMFFLGLFVLVHGLEKTGVIHW
                     MAERLLEATRGDFKITALAILWASAILSAFIDNIPFVATMIPLIKATAPTFGGDAALV
                     PLWWALSLGACLGGNGTLIGASANLVVAGLAERAGTGFRFVEFLKIAFPLMLVSIVIS
                     HVYVYARFL"
BASE COUNT          625 a         1104 c         1144 g          567 t
ORIGIN      
        1 gcgaggtgct cgtagatcgc gtcgcggatc gtgaccaggt acaggaacgc ggtcatcgcc
       61 ccgagctcca tcagcgcggc ggcggtgcag gtcatgtggt cggcgatgcg cgagtattcc
      121 gccagcagcg tgcgcaggta gcggcagcgc ggcgtgatct ccacgcccat caggcgttcg
      181 accgcgtcgc agtacgcaaa gtcattgatc agcgccgagc agtagttcag ccggtcgacg
      241 tacgggatca ggttgtgcca ggtgtggtcc tcgcactcct tctcgaaacc gcggtgcagg
      301 tagcccggat gaatatcggt gcggacgacg cgctcgccgt ccagctcggc gatgatctgc
      361 accgtgccgt gcgtcgccgg atgcgacgga ccgatgttga cgatcacgtg atcgtcgctg
      421 atcggcttgt cgaaagccgg acggacggga ttgggaaggt tctggtccat ggtctacgag
      481 cgatacggca ccagcggctg ctcctgctgc ttcggatagt ccttgcgcag cggatgaccc
      541 tgaaatcctt cgtacagcag caccggacgc aggtcggggt tgccgcggaa ccggatgccg
      601 tacatgtcgt ggcattcgcg ctccatgtag cgcgccgagc cgtacagcga caccagcgaa
      661 tccacggtcg gatcggactc gtcaacgcgc gccttcacgc gaatgcgcgc gaagtgccgc
      721 gtcgagtaga agtgccacac gacctcgaag cgcggcgcct ggccgagcca gtcgacggcg
      781 gtcacgtcgg tgaagaggtc gaaatcgaac tcgctcttga gcttctgcgc ggtggcgagc
      841 accgcgtcgg gcgcgatgaa gacgaccagc gtgccgtgcg acacgtccgc cgcctgtgcc
      901 gccggcagac gctccttcac ccgtgcgaac acgtccatcg tcagaattcc cgcgtgatga
      961 tcggatgttt gccgcgcgcg atcttgtcct gcagctgcat gatcccgtcg atcaccatct
     1021 cggggcgcgg cgggcagccc gggacgaaca cgtcgaccgg aatgatcttg tcgatgccgg
     1081 cgaccgcggc gtaattctgg tagaagccgc cgctcgaggc gcacacgccg aacgccatca
     1141 cccacttggg ctcgcacatc tgctcgtaga ccttcagcag gatgggcgcc tgcttgtgcg
     1201 tgacggtgcc gaccaccatc agcaggtcgg cctggcgcgg cgagaaacgc ggcagcgcgg
     1261 cgccgaagcg gtcggtgtcg tacggcgtgc cgctgaccgc catgaactcc atcgcgcagc
     1321 aggcggtgac gaacgggtag gggaacagcg agaacttgcg tgcccagccg gccagttcgt
     1381 ccttcttggt cgtgatgtat tcgaacgcgg cgctcttgcc gccctgcgcc ggcacgatcg
     1441 ggatcgattt cgcgttcatc actcggtcac cgcttcgagc agccgcgcct tgtagacgta
     1501 cagcaggatc agcaccagca gcgccatgaa gatcgcgaag gtggccagca tgaaaccggt
     1561 catcggctgc gcaccgagcg cccagatgaa caggaatacg gtttcgaggt cgaacaggat
     1621 gaagacgatc gccaccgcgt agtacttggc cggcacggcc ttgacgttct gcgtttcgac
     1681 cggcgtggcg ccgcactcga acggctccag cttggacgcg gtcatcgccg gcttcggacc
     1741 cagcagcttg ttgagcagca gcgtgagcgc gacgaagccc aggatggcga ccatgtacag
     1801 caggaagacg gcataggggc tcatcgcgtt tcctcggcgt ggcgcacgct cgtttggcgc
     1861 ggcccttgca tcgtcagtcg cagggccatg gctggctcct tggtgctccc tggatttcgc
     1921 catcggtcgc ggctgcgacc ggtcacgcgg cgattgggtg caaccgtagg cgcagtcgac
     1981 ccgccccctt ttgatggcgg tcaagcgaac gcggtcgcac gaccggggtg tttgatttag
     2041 gtaccgcgcc ccggagactc agaggaagcg cgcgtacacg tacacgtggc tgatgacgat
     2101 cgacacgagc atcagcggga acgcgatctt gaggaactcg acgaagcgaa aaccggtgcc
     2161 ggcgcgctcc gcgagccccg cgaccacgag gttggccgag gcgccgatca gcgtgccgtt
     2221 gccacccagg cacgcgccga gcgagagcgc ccaccacagt ggcaccagcg ccgcgtcgcc
     2281 gccgaaagtc ggcgcggtgg ccttgatcag cggaatcatc gtcgccacga acgggatgtt
     2341 gtcgatgaag gccgacagga tcgccgaggc ccacaggatc gccagcgccg taatcttgaa
     2401 gtcgccgcgg gtcgcctcga gcaggcgctc tgccatccag tggatcacgc cggtcttctc
     2461 cagcccgtgc accagcacga acaggccgag gaagaacatc agggtgatcc attccgcctc
     2521 ggacagcgcg gcgtgcacgt tgtgcgactg cttttccggg tcacggccga ggttatccag
     2581 cagaagcagc gcggcggcgc cgaagatcgc gatcgtcgcg ggttcgatcc tgatctgccg
     2641 ctgcagcatg aagcccacga tcaccagcgc gatcacggtc agcgactgct tgagcagtcg
     2701 cacgtcggtg atcgcctcgc gctcgttgaa gcccagcaca cgctggcggt gctccgcgtc
     2761 ggcgtgcaga tgctttcccc agatgtacca cagcggcacc agcgtggcga ccagcaccac
     2821 gatgatgacg ggcgtcagcg cgatggcgaa gtcgttgaag gtgagcccgg tcgcggagcc
     2881 gatcatgatg ttgggcggat cgccgatcag tgtggccgtg ccgccgatgt tggaggcgaa
     2941 gatctgcgcg aacaggtacg gatagggctt gaccttcagt tcgtcggtga tcagcagcgt
     3001 gacgggcacc gtcagcagca cggtcgtcac gttgtcgagg aacgcggaga acaccgcggt
     3061 cacgatcgac atcatcagca ggatgcccca cgggcgggcg tcgaccttct tggccgacca
     3121 gatcgccaca aactggaaca ggcccgaccg ccgcgtgatg ttgacgatca gcatcatccc
     3181 cagcagcagg ccgagcgtgt tgaagtcgac cgaccccatc gcctccccct gcgtgaccac
     3241 gccgagcagg atcaggagcc ccgcgccgag caacgcgagg atcgcgcggt tgaccttctc
     3301 ggtcatgatc agcacgtagg tcgcgacgaa cagcgccacc gcgatccaca gcggattcag
     3361 cccgaagatc gcggtggccg catgggtagc ctggccgtgc atgtgtctcc tccgaccgaa
     3421 gtgcccggcg ctcgcgccgg
//
LOCUS       DBNHBY010000169         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1768545, whole genome shotgun sequence.
ACCESSION   DBNHBY010000169 DBNHBY010000000
VERSION     DBNHBY010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1768545"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1861
                     /locus_tag="AC16HB_04130"
     CDS             <1..>1861
                     /locus_tag="AC16HB_04130"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606010.1"
                     /translation="TAASPSGSASPPPAADAGLASLKGVGEVFRYTRHALELVWSTSR
                     RLTFALAALTLIAGVLPAAIAFVGARIVDAVVAAIAAHSASGAVPLDTVLGYVVLEGA
                     LVAALAAAQRGLSTCQSLLRAQLGQRVNEMILEKALTLDLRHFEDSEFYDKLTRARRE
                     ASSRPLSLVVRSFGLVQNAIALLSFGTLLAQFSPWAVAVLLLAGLPSFIAEAKFSGDA
                     FRLFRWRSPETRMQLYLETVLAREDHAKEVKLYELGPRLLDRYRTIFRKVFREDRALT
                     IRRDTWGFFLGLVSTAAFYAAYAWVAVSAVRGQITLGEMTMYVLLFRQGQAAVTASLS
                     AIGGMYEDNLYLSTLYEYLDTPVTPGTGTATTGAAPGTGLRFEHVSFTYPGASSPAVR
                     DISFDLRPGQSLALVGENGSGKTTLIKLLTRLYTPDSGRITLDGTDLADWQPAALRRR
                     VGVIFQDFARYQLSVGENIGVGDVAHFDDEARWREAAALGMAAPFIEELPAGYATQLG
                     KWFRDGRELSGGQWQKIALSRAFMRREADILVLDEPTAAMDARAEAQVFEQFQALARD
                     RMVILISHRFSTVRRADQILVIENGRILERGSHEALMRIGGQYAALFALQARG"
BASE COUNT          260 a          656 c          639 g          306 t
ORIGIN      
        1 ccaccgccgc atcgccatca ggctcggcgt cgccgcctcc cgcggctgac gcggggctcg
       61 cctcgctcaa gggcgtcggc gaggtcttcc gctacacgcg tcacgcgctc gagctggtct
      121 ggtcgacgag ccgccggctc accttcgcac tcgcagcgct gacgctgatc gctggcgtcc
      181 tgcccgccgc catcgccttc gttggcgccc gcatcgtcga cgctgtcgtc gcggccattg
      241 ccgcgcacag cgcttccggc gcggtaccgc tcgacaccgt actcggctac gtcgtgctcg
      301 aaggcgcact ggtcgcagcg ctcgccgccg cgcagcgcgg gctgtcgacg tgccagtcgc
      361 tgctgcgcgc gcagctcggc cagcgcgtca acgagatgat cctcgagaag gcgctgaccc
      421 tcgacctgcg tcacttcgag gactccgagt tctacgacaa gctgacgcgc gcgcgacgcg
      481 aggcgtcgag ccgaccgctg tcgctggtcg tccgcagctt cggcctggtg cagaacgcga
      541 tcgccctgct ctccttcggc acactgctcg cgcagttttc gccgtgggcc gtcgcggtgc
      601 tgctgctcgc cggcctgccc tcgttcatcg ccgaagcgaa gttctcgggc gacgcgttcc
      661 ggctgttccg ctggcgctcg cccgagacgc gcatgcagct ctatctcgag acggtgctgg
      721 cgcgcgaaga ccacgcgaag gaggtcaagc tctacgagct ggggccgcgc ctgctggacc
      781 gctaccgcac gattttccgc aaggtgttcc gcgaggaccg cgcgctcacc atacgacgcg
      841 acacctgggg cttcttcctc gggctcgtca gcactgcggc gttctacgct gcgtacgcct
      901 gggtggcggt cagcgccgtg cgcggccaga tcacgctcgg cgagatgacc atgtacgtgc
      961 tgctgttccg ccaggggcag gccgccgtca cggcgagcct gtcggccatc ggcgggatgt
     1021 acgaagacaa tctctacctt tcgacgctgt acgagtacct cgacactccg gtgacgcccg
     1081 ggacgggcac agccacgaca ggcgcagccc cgggcacggg gctgcggttc gagcacgtca
     1141 gcttcacgta tcccggcgcg tcgtcaccgg cagtgcgcga catcagcttc gacctgcgtc
     1201 ctggccagag cctcgcgctg gtcggcgaga acggctcagg caagacgaca ctgatcaagc
     1261 tgctgacgcg gctgtacacg ccggactcgg ggcgcatcac gctggacggc accgatctcg
     1321 cggactggca gcccgcggca ctgcggcgtc gcgtcggggt catcttccag gatttcgcgc
     1381 gttaccagct gtccgtcggc gagaacatcg gcgtgggcga cgtggcgcat ttcgacgacg
     1441 aggcacgctg gcgcgaggcc gctgcgctcg gcatggccgc gccgttcatc gaggaactgc
     1501 cggccggcta cgcgacgcag ctcggcaagt ggttccgcga cggtcgcgag ctgtcgggcg
     1561 gccagtggca gaagatcgcg ctgtcgcgcg ccttcatgcg gcgcgaggcc gacatcctcg
     1621 tgctggatga acccactgcg gcgatggatg cgcgcgccga agcgcaggtg ttcgagcagt
     1681 tccaggcgct cgcgcgcgat cgcatggtga tcctcatctc gcaccggttc tcgacggtgc
     1741 ggcgcgcaga ccagatcctg gtcatcgaga acgggcgcat cctggagcgc ggcagccacg
     1801 aggcgctgat gcggatcggg gggcagtacg cggcgctgtt cgcgctgcag gcgcgggggt
     1861 a
//
LOCUS       DBNHBY010000170         1536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_549993, whole genome shotgun sequence.
ACCESSION   DBNHBY010000170 DBNHBY010000000
VERSION     DBNHBY010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_549993"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..1246
                     /locus_tag="AC16HB_04135"
     CDS             455..1246
                     /locus_tag="AC16HB_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5606011.1"
                     /translation="MPAESRVPGGIAILDLGPGEQPPGEVWFGKYRAPVVPDGDTWKA
                     IVGIPLATAPGPQTARLVTPATDDADRTLKFDVAPKAYATQKLTVEPRKADPLPEDLK
                     RIEAESARTEKALSMFSTDFTPTWVWDPPVPGTQSSSFGLRRVFNGLPRNPHTGMDIA
                     APTGTPIRSPAAGRVVEAGDFFFNGNTLFVDHGLGVLTMYCHLSRIDVKVGDTVEAGT
                     VLGLVGATGRVTGPHLHWGVAVNRALVDPALFLRPQGTPAVAAGK"
     gene            complement(1292..>1536)
                     /locus_tag="AC16HB_04140"
     CDS             complement(1292..>1536)
                     /locus_tag="AC16HB_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606012.1"
                     /translation="SLCTAYAKFRTKSALVIGVQSDMLYTIDQQEQIAAQLRDAGREV
                     EFHAFPSLQGHDAFLVDLARFEPAICDFLGRIEPGR"
BASE COUNT          248 a          561 c          515 g          212 t
ORIGIN      
        1 accgcggcta cgcgatcgtg actgatgcgt cgaccggccg tgtgctggtc gatgcggcgg
       61 gcgtgacgcc gggcacggcg atcgacgcgc ggctcgcgca aggcacgctg cgggcgacgg
      121 tgacgcaggc aacgcgcggc gccgtcaaaa taccgaagaa accgcagaag caatgaacca
      181 agcaggcaaa caagcacgcg cggcagcgac gtggcgcacc gtgtcgggtg ctcttccggc
      241 gggccgtccg ccgcacggac gcggcggccg gagcctacat ggacgtattc acggcgtccc
      301 gccggaagag cacgcggcgc ggggcgactc gtcgcgctct cattcgtcgc ttgcgcatcg
      361 tgctcgcacc tcagcggcgc acgtcttccg cagtctgctg gtcgcagccg cgttcgctct
      421 cgcgccgccg gcgcacgcca ccgagtccgc gacgttgccc gccgaatcgc gcgtgcccgg
      481 gggcattgcc atcctcgacc tcggcccggg cgaacagcct cccggcgaag tctggttcgg
      541 caagtaccgc gcacccgtcg tgcccgacgg cgacacgtgg aaagcgatcg tcggcatccc
      601 gctcgccacg gcgcccggtc cgcagaccgc acgcctggtc acgcccgcaa ccgacgacgc
      661 cgaccgcacc ctgaaattcg acgtcgcgcc aaaggcctat gcgacgcaga aactgaccgt
      721 cgagccgcgc aaggccgacc cgctgcccga ggacctgaaa cgcatcgagg ctgaatccgc
      781 acggacggag aaggcattgt cgatgttttc caccgacttc accccgacgt gggtctggga
      841 cccgccggtg cccggcaccc agtccagctc gttcgggctg cgccgcgtct tcaacggcct
      901 gccgcgcaat ccgcacaccg gcatggacat cgcggccccg accggcacgc cgatccgctc
      961 gcccgccgcc gggcgcgtcg tcgaggccgg cgacttcttc ttcaacggca acacgctgtt
     1021 cgtcgaccac ggcctcggcg tgctgaccat gtactgccac ctgagccgca tcgacgtgaa
     1081 ggtcggcgac accgttgaag ctggcacggt gctggggctg gtgggggcga cgggccgcgt
     1141 gaccggcccg cacctgcact ggggcgtcgc ggtgaatcgc gcgctggtgg accccgcgct
     1201 gttcctcagg ccacagggca cgcctgccgt cgcagcaggc aaataggcag cgccacgcct
     1261 cgggcagatt gcagcgcctg gcgcgtgcga actagcggcc gggttcgatc ctgccgagga
     1321 agtcgcagat cgccggctcg aagcgcgcga ggtcgaccag gaacgcgtcg tgaccctgca
     1381 gcgacgggaa cgcgtggaat tccacctcac gcccggcgtc gcgcagctgc gccgcgatct
     1441 gctcctgctg atcgatggtg tacagcatgt ccgactgcac gccgatcacg agcgcgctct
     1501 tcgtgcggaa cttcgcgtac gccgtgcaca gcgaac
//
LOCUS       DBNHBY010000171        12838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_414868, whole genome shotgun sequence.
ACCESSION   DBNHBY010000171 DBNHBY010000000
VERSION     DBNHBY010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_414868"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16HB_04145"
     CDS             <1..183
                     /locus_tag="AC16HB_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5606013.1"
                     /translation="VAQELAGAAAVSDAIDAPRVERYGSIFRVLIGDLVDRAAALALA
                     QRVERILSRETSVFAR"
     gene            211..1701
                     /locus_tag="AC16HB_04150"
     CDS             211..1701
                     /locus_tag="AC16HB_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5606014.1"
                     /translation="MNLADLRHFVERRWDEELIPRLVDYVKVPAKSPAFDASWSAHGY
                     LQAVVQDAYAWGAAQKIKGLAIEIVSIEGRTPCIFFDVPATGTYGNQKTVLFYGHLDK
                     QPEMFGWREGFGPWTPVIEDGKLYGRGSADDGYAIYCALTVIAALDAQGAPRPRCVGI
                     IESCEESGSPDLPAYLELLAPRFGDVTLVAGLDSGCGNYEQLWMTTSLRGMAGGKLTV
                     EILGEGVHSGHASGIVPSSFRIARQLLNRVDDPATGRVLDPAFHTEIPQERLDQAQVA
                     GQVLGDKVWTEFPWIACARGPGTENELLPQDLRQHVHEGLPTSQPMSRDPVVAILNRT
                     WRPALSITGAGGLPSIESAGNVLRPKTTLKLSLRIPPTVDGERATRRLKELLEADPPY
                     NAKVTFDADWGATGWNAPETASWLKQAADDASRAVFGKGSAWTGSGGTIPFMNMLGDR
                     FPRAQYLITGVLGPKSNAHGPNEFLHIPYVKQISAAVARVITALPG"
     gene            1723..3783
                     /locus_tag="AC16HB_04155"
     CDS             1723..3783
                     /locus_tag="AC16HB_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5606015.1"
                     /translation="MTDAPLLDRLGALARRRPVEMAVALLAIAALAVWIYSGVRNALT
                     GLEANTLRTLVGSQSVTLDAWISEKKLNVQRWATDPRLVDAAAPLLAAAARGPDELQR
                     ACRGEPGARLIAVIDTLRQPDIAASVHLFDLEGRVLAARNPAKCGWPLHDAMRATFDR
                     VRAGATVFSGSRSDAERVGAVPGVEAGPPRVWIAAPVRDGHGDVVAVLDIGKPADERF
                     SQLFEAARTGATGEAYAFDAAGRLLTASRFVATLEGAGRIKAGSSPIGQLRLSDPGAE
                     SGAPPLTALIAHALAVRRDGVKAGEMLQPYRNYVGEPVIGAWRWFDDYDFGVAVEIAE
                     REAFAPLARLETAFLGLGGVVSVAVFGLVVALLRMLRMQQEVEAAKRVGNYELLEEIG
                     QGGFARVYRARHRLLKRPTAVKIVELRLANDETLARFDREAKLASQLSHPNTVEVFDF
                     GRTPEGQPFLAMEFLDGLTLQQVVERHGALSAPRVAHVLRGIAGSLAEAHARGLVHRD
                     IKPANVMLCRRGGEFDVVKVLDFGLVKDTHTEATRDLTHALRVLGTPSYMAPERIEHP
                     DSADPRSDLYALGAVGSFLLRGAPPFEGANDLALAYQVVHVAPPPLPATVPTALAQLI
                     ERCLAKSPAARPQSATEVIDALAPLLAQTTWTFHEARAWWESRDGVDLGTTVQPRSFP
                     ISAS"
     gene            complement(3750..4652)
                     /gene="rsmI"
                     /locus_tag="AC16HB_04160"
     CDS             complement(3750..4652)
                     /gene="rsmI"
                     /locus_tag="AC16HB_04160"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR5606016.1"
                     /translation="MERKPFLNDDPLIALSGVADQDLPAGALYVVATPIGNAADITLR
                     ALWVLSQMDAIACEDTRTTRPLLARYRIDVPLLAAHEHNERTVAARIDDRLSRGQRVA
                     LVTDAGTPAVSDPGARLVGALRAAGHRIVPVPGASSVTAALSAAGLSGAPWVFIGFLP
                     SGAKQRAQTLRAAAATGHAAIILEAPHRIAATAQEITAAFDPAQRVIVARELTKEFES
                     IVATTIIDLPREIARREARGEYVLLIDAPAPAPERALDELTLRWLHALGAELPAARAA
                     ALAAQVTGLPRARLYDALIGKLRG"
     gene            4651..5757
                     /locus_tag="AC16HB_04165"
     CDS             4651..5757
                     /locus_tag="AC16HB_04165"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR5606017.1"
                     /translation="MLVRLLSLWMTAACLSLSAHAQAPTNPAPTPPPPASEAAIALIV
                     PGADTPFGRAGEAVREGFIAAHRATRAPVAIQVIETDASAAQAVAAVQAARERGARLV
                     VGPLTRNAVNGVVERGDAGIPIVLLNVPDHAGAIADSQLAFGLGVEDEAALLTRASLR
                     EIDPGATASAARRCVVVVGDGALARRIAAAFVAALKDAGAPTVTLDYAQHGADGVARE
                     IMRAPVTSVFLALGSRDATQVRTRLPIDAPLYATSQINVGGGMLLANDLEGVRFVDMP
                     WLIEPERPTVKAYPRSADGSSTEMQRLYALGIDAYRVALEWLAHRTRFEIDGVTGWLR
                     VDRARSPRVERTPGFAVFREGRIERSDALREVAR"
     gene            5770..6174
                     /locus_tag="AC16HB_04170"
     CDS             5770..6174
                     /locus_tag="AC16HB_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5606018.1"
                     /translation="MSFLRRRPGLRTPKQRAGDAAEQAACERLLASGCRILARNVRYR
                     EGELDIVAQDGATVVFVEVRLRNDERFGGAAASVDRFKRRRLARAAQHYLVEHFGGRS
                     SAALPPCRFDVITADDGGVGEWIRNAFSETGD"
     gene            6175..6819
                     /locus_tag="AC16HB_04175"
     CDS             6175..6819
                     /locus_tag="AC16HB_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5606019.1"
                     /translation="MRKILVRWAAAGAVVSAVVGCAPLLVGGVVAGGAMVATDRRSVG
                     TQLDDEAIERRVNRALAEKFPRDSANINVVSYNRKVLLTGEVTTPQAKDSAEQIALKT
                     DNVREVLNELEVGALSTIGARTDDTATTAKVRAALINAKEVPSAAIHVVTEHGVVYLL
                     GRVTESEGTYAGTVAARVSGVKRVVKSFDYLTQAEVTDLQSKPAQPAPTDAPRK"
     gene            6931..7518
                     /locus_tag="AC16HB_04180"
     CDS             6931..7518
                     /locus_tag="AC16HB_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5606020.1"
                     /translation="MGWLIFLGLIALLIFWAIGAYNRLVGLRNQFKNAFAQIDVQLKR
                     RYDLIPNLVETAKAYMKHERETLEAVIKARNSAVTANQAAAADPGNAGAMQGLVAAEG
                     ALRGALGKLFALAEAYPDLKANQNMMQLTEELTGTENKIAFARQAFNDAVMSFNTAVE
                     QFPSNVIASAFSFKPAELLAATEAPEEKKPVKVQF"
     gene            7550..8686
                     /locus_tag="AC16HB_04185"
     CDS             7550..8686
                     /locus_tag="AC16HB_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5606021.1"
                     /translation="MNFFEHQDQARRNTKRLVVLFVLAVIGVVIAVNVVGALVYVSVM
                     PHPRGVPGAAALPNGFFAVNTLVTLALIGGGTWWETARLSAGGAAVAKMVDARLIDPS
                     TSDLLERRLVNVVEEMAIASGIPVPRVYVMDHENTINAFAAGHSINDAIIAVTRGTLT
                     RLSRDELQGVIGHEFSHILNGDMRLNLRLIGVLFGLVMIAMFGRFMMQMSRGSRDRGT
                     GAFVIAGLVLWIVGYIGVLFGRIIKSAVSRQREFLADASSVQFTRNPDGIGGALRKIG
                     GLGGVGSMAPAVAAPDPALPQMVDTAHELRARGVRVDVGSQIGHAHAETLSHMFIAPA
                     RLAFAGGWLATHPPLEERIERIYGRALPFLPAPEQPLQLALDAA"
     gene            8789..9646
                     /locus_tag="AC16HB_04190"
     CDS             8789..9646
                     /locus_tag="AC16HB_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606022.1"
                     /translation="MADVHAAIGGSPPQAQDFARRLTQERTDAVEAILTDTTGVQLLV
                     LGMLIEKGQPFSGQQRQLVTEAFGATAAQRVDALHELMQQLPPGRRLPLLDRAMPALR
                     KLSMESAERLLMLCHALIAADGRVTLPEFLLFTVLGRRIGRNAQRAVPVRYRSVAERA
                     ADAALVLSLIAAVRSPEKAEHAFNAGALLLPNVEVARTDAAAIRLDEVSAALERLNQL
                     APLAKPQLIKACTAAAFVDGQTNWKAASCLRTICAALDAPLPPQIDGAPEEDTPAAAV
                     AQPVQGELL"
     gene            complement(9656..10375)
                     /locus_tag="AC16HB_04195"
     CDS             complement(9656..10375)
                     /locus_tag="AC16HB_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR5606023.1"
                     /translation="MLEHEFSADEVRRALVIGSALAAALGSRAAFALSLADLTQKDAS
                     AGVKAALEKGATVAVELLGRTDGFWGNDKVRIPLPEWLQRAEGALKLVGRGKDIDDLK
                     LGVNRAAEQAVPQAKALLVSAVRNMTVQDAKGILTGGNDAVTKFFAAKTREPLSGKFL
                     PIVTKVTDRIGLARQYNTLAAKGEQLGLVRGDQSRIERHVTAKALDGMYFMIGEEEKK
                     IRQDPVGTGSEILKKVFGAIR"
     gene            10364..10555
                     /locus_tag="AC16HB_04200"
     CDS             10364..10555
                     /locus_tag="AC16HB_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606024.1"
                     /translation="MLEHPVLLLQRHRVAALIVVLLAMGAVFTAAAVLSKPRAPDLAP
                     VNLDGERERWRICAGRWCG"
     gene            10683..10886
                     /locus_tag="AC16HB_04205"
     CDS             10683..10886
                     /locus_tag="AC16HB_04205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5606025.1"
                     /translation="MLGNRERNRLPFPIALDVQGEIARAFDAARVTPTTFLIDKRGRI
                     VKRYIGEPDLTEMRERIEKPRAG"
     gene            complement(10883..11845)
                     /gene="lipA"
                     /locus_tag="AC16HB_04210"
     CDS             complement(10883..11845)
                     /gene="lipA"
                     /locus_tag="AC16HB_04210"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5606026.1"
                     /translation="MSTSPDPFAKKKGDAKTGVVFKKIPIAVEARTTLRKPDWIRVRA
                     GSPTTRFYEIKDILRSHKLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCTFCDV
                     AHGRPEPLDADEPINLAQTIAALKLSYVVITSVDRDDLRDGGAQHFVDCIRAVRERSP
                     ATKIEVLVPDFRGRLDRALEILEAAPPDVMNHNLETVPRLYKVARPGSDFAHSLKLLQ
                     EFKRRVPSVPTKSGLMLGLGENDDEILQVLRDLRAHDVDMLTLGQYLAPSTAHFPVQR
                     YVHPDTFAMFEREAVALGFSHAACGPLVRSSYYADQQAHKAGVV"
     gene            complement(11842..12486)
                     /gene="lipB"
                     /locus_tag="AC16HB_04215"
     CDS             complement(11842..12486)
                     /gene="lipB"
                     /locus_tag="AC16HB_04215"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR5606027.1"
                     /translation="MRALVVRSLGRADYLATWQAMREFTARRTADMPDELWIVEHPPV
                     FTLGQAGRREHVLDSGDIPVIATDRGGQVTYHGPGQVVAYPLLDLKRLGIFVRELVFR
                     IEQSVIQVLDSYGIAGQRVKGAPGIYVPFESTSSGEFAGLAKIAAIGIKITRGCSYHG
                     VAFNVAMDLAPFSRIDPCGYAGLATVDLRRIGVSTTWADAAQRLSERLASHFSR"
     gene            complement(12510..12773)
                     /locus_tag="AC16HB_04220"
     CDS             complement(12510..12773)
                     /locus_tag="AC16HB_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HIR5606028.1"
                     /translation="MQTESSLSFPTDFPIKVMGKRVNGFAQAVVDVVQKHAPDFDPAT
                     LELRVSKAGNYLSVTATVNATSREQLDDLYRALTSHPLVKVVL"
BASE COUNT         1916 a         4411 c         4559 g         1952 t
ORIGIN      
        1 gtcgcgcagg aactggccgg tgccgccgcg gtctccgacg ccatcgacgc tccgcgcgtc
       61 gagcgctacg ggagtatctt tcgcgtcctg atcggcgatc tcgtcgatcg cgccgcagcg
      121 ctggcgctgg cgcagcgcgt cgagcgcatc ctgagccgcg aaacctctgt gttcgcgcgc
      181 tgaagcgaac ccatccgcaa ggagaacccg atgaatcttg ccgacctcag acacttcgtc
      241 gagcgccgct gggacgagga actgatcccg cgcctggtcg attacgtgaa ggtgcccgcc
      301 aagtcgcccg cgttcgacgc gagctggagc gcgcacggct acctgcaggc agtcgtgcag
      361 gacgcgtatg cgtggggtgc ggcgcagaag atcaagggcc tcgcaatcga gatcgtgtcg
      421 atcgaaggca ggacaccgtg catcttcttc gacgtgcccg ccaccggcac gtacggcaac
      481 cagaagacgg tgctgttcta cggccacctc gacaagcagc ccgagatgtt cggctggcgc
      541 gagggcttcg gcccgtggac gccggtgatc gaggacggca agctgtacgg gcgcggttcg
      601 gccgacgacg gctatgcgat ctactgcgcg ctcaccgtga tcgccgcgct cgacgcgcag
      661 ggtgcgccgc ggccgcgctg cgtcggcatc atcgagagct gcgaggagag cggctcgccg
      721 gatctgcccg cgtatctcga gctgctggcg ccgcgcttcg gcgacgtgac gctggtggct
      781 ggactcgact ccggctgcgg caactacgaa cagctgtgga tgacgacctc gctgcgcggg
      841 atggccggcg gcaaactgac ggtcgagatc ctcggcgaag gcgtgcactc cgggcacgcg
      901 agcggcatcg tgcccagttc gttccgcatc gcgcggcagc tgctaaatcg tgtcgacgac
      961 cccgccacgg gccgcgtgct cgatcccgcg ttccacaccg agataccgca ggagcggctc
     1021 gaccaggcgc aggtcgcggg ccaggtgctc ggcgacaagg tgtggaccga gttcccgtgg
     1081 atcgcctgcg cgcgcgggcc cggcacggaa aacgaactgc tgccgcaaga cctgcgtcag
     1141 cacgtgcatg agggcctacc gacttcgcaa ccgatgtcgc gcgatccggt cgtcgcgatc
     1201 ctgaaccgca catggcggcc cgcactgtcg atcaccggcg ccggcgggct gccgtcgatc
     1261 gaatcggcgg gcaacgtgct gcggccgaag acgacgctga agctctcgct gcgcattccg
     1321 cccacggtcg acggcgagcg ggcgacgcgc cggctgaagg aactgctcga ggccgatccg
     1381 ccgtacaacg cgaaagtgac gttcgacgcc gactggggcg cgaccggctg gaacgcgcct
     1441 gagaccgcgt cgtggctgaa gcaggcggcg gacgacgcgt cgcgggcggt gttcggcaag
     1501 gggtccgcat ggaccggctc cggcggcacg atcccgttca tgaacatgct gggcgacagg
     1561 tttccgcgcg cgcagtacct gatcaccggc gtgctcggtc cgaagtccaa cgcgcacggc
     1621 cccaacgagt tcctgcacat cccctacgtg aagcagatct cggccgcggt ggcacgcgtg
     1681 atcaccgcgc tgcccgggta acttcgggaa cgggcgtcaa cgatgactga cgccccgctt
     1741 cttgaccgtc tcggtgccct ggcgcgcagg cggccggtcg agatggcggt cgcgctgctc
     1801 gcgatcgcgg cgctcgccgt ctggatctac agcggcgtgc gcaacgcgct gacggggctc
     1861 gaagcgaaca cgctgcgtac gctggtcggc agccagagcg tcacgctcga tgcctggatc
     1921 agcgagaaga agctgaacgt gcagcgctgg gcgaccgacc cgcgcctagt cgacgcggcc
     1981 gcgccgctgc tggccgctgc cgctcgtggg cccgacgagc tgcaacgcgc ctgccgcggc
     2041 gagccgggcg cgcgtctgat cgccgtgatc gacacgttgc gacagcccga catcgccgct
     2101 tcggtccacc tgttcgatct cgaagggcgc gtgctggccg cgcgcaaccc ggccaagtgc
     2161 ggctggccgc tgcacgatgc gatgcgtgcc acgttcgatc gggtgcgagc cggtgcgacg
     2221 gtcttcagcg gctcgcgcag cgatgcggaa cgagtcggcg cggtaccggg cgtggaggcc
     2281 gggccgccgc gggtctggat cgccgcgccg gtgcgcgacg gccacggcga tgtcgtcgcg
     2341 gtgctggata tcggcaagcc ggccgacgaa cgcttctcgc aactgttcga ggcggcgcgc
     2401 accggcgcca ctggcgaggc gtacgcgttc gacgcggccg gccggctgct gaccgcaagc
     2461 cgcttcgtcg cgacgctcga gggcgccggt cgcatcaagg ccggcagcag cccgatcggg
     2521 cagctgcggc tgagcgatcc cggcgccgaa tccggcgcgc cgccgctgac cgcattgatc
     2581 gcgcatgcgc tcgccgtgcg ccgcgacggc gtcaaggccg gcgagatgct gcagccgtat
     2641 cgcaactacg tcggcgagcc cgtgatcggc gcatggcgct ggttcgacga ctacgacttc
     2701 ggtgtcgcgg tcgagatcgc cgagcgcgag gcgttcgctc cgctggcgcg cctggagacc
     2761 gcgtttctcg ggctgggcgg tgtcgtcagc gtcgcagtgt tcgggctggt ggtcgcgctg
     2821 ctgcgcatgc tgcggatgca gcaggaggtc gaggcggcga agcgcgtcgg caactacgag
     2881 ctgctcgagg agatcgggca gggcggcttt gcgcgcgtgt accgcgcacg ccatcggttg
     2941 ctcaagcggc cgacggccgt gaagatcgtc gaactgcgcc tggccaacga cgaaacgctg
     3001 gcccgcttcg atcgcgaggc aaagctggcg agccagctgt cgcacccgaa cacggtcgag
     3061 gtgttcgact tcggccgcac gcccgagggc cagccgttcc tcgccatgga gttcctcgac
     3121 gggttgacgc tgcaacaggt cgtcgagcgg cacggcgcgc tgtcggcgcc gcgtgtggcg
     3181 cacgtgctgc gcggcatcgc tggatcgctg gccgaggcgc acgcgcgcgg gctcgtgcac
     3241 cgggacatca aacctgccaa cgtgatgctg tgccggcgcg gcggcgaatt cgacgtggtc
     3301 aaggtgctcg atttcggcct ggtcaaggac acccacaccg aagccacgcg cgatctgacg
     3361 cacgcgctgc gcgtgctcgg cacgccgtcg tacatggcgc cggagcgcat cgagcatccg
     3421 gacagcgccg acccgcggtc ggacctgtac gcgctcggcg ccgtcggctc gttcctgctg
     3481 cgcggcgcgc cgccgttcga gggtgcgaac gatctggcgc tcgcctacca ggtcgtgcac
     3541 gtggcgccgc cgccgcttcc cgcaaccgtg ccgaccgcgc tcgcgcaact gatcgagcgc
     3601 tgcctggcga aatcgcccgc cgcgcggccg cagtccgcaa ccgaagtgat cgacgcgctc
     3661 gccccgctgc ttgcgcagac gacgtggacc ttccacgagg cgcgcgcgtg gtgggaatcg
     3721 cgcgacggcg tcgacctggg cacgacggtt cagccgcgca gcttcccgat cagcgcgtca
     3781 tagagccgcg cgcgcggcag cccggtgacc tgcgccgcca gtgcagccgc gcgtgccgcc
     3841 ggcagctccg cgccgagcgc atgcagccag cgcagcgtga gctcgtccag ggcgcgctcc
     3901 ggcgcgggcg cgggcgcgtc gatcagcagc acgtattcgc cgcgcgcctc gcggcgcgcg
     3961 atctcgcgcg gcaggtcgat gatcgtggtg gcgacgatcg actcgaactc cttcgtcagc
     4021 tcgcgcgcga cgatcacgcg ctgcgccgga tcgaacgccg cggtgatttc ctgcgcggtg
     4081 gcggcgatcc ggtgcggtgc ctcgaggatg atcgccgcgt ggccggtcgc ggcggccgcg
     4141 cgcagcgtct gcgcgcgctg cttcgcgccg ctcggcagga acccgatgaa gacccatggc
     4201 gcgccgctca gaccggcggc gctgagcgcg gcggtcacgc tgctcgcccc gggcaccggc
     4261 acgatccgat ggcccgcggc gcgcagcgcg ccgaccagcc gcgcgcccgg atcggacacc
     4321 gccggcgtgc cggcatccgt gaccagcgcc acgcgctgtc cgcgtgagag acggtcgtcg
     4381 atgcgcgcgg cgaccgtgcg ctcgttgtgc tcgtgcgcgg ccagcagcgg cacgtcgatc
     4441 cgatagcgcg cgagcagcgg ccgcgtggtg cgagtatcct cgcaggcgat cgcgtccatc
     4501 tgcgatagca cccacagtgc acgcaacgta atgtccgcgg cattgccgat cggcgtggcg
     4561 accacgtaca gcgcgccggc tggcaagtcc tgatccgcca cgccgctcaa agcgatcagt
     4621 ggatcgtcgt tcaagaacgg cttgcgttcc atgttggttc gattgctctc gctgtggatg
     4681 accgccgcgt gcctgtcatt gtcggcgcac gcgcaggcgc cgacaaaccc ggcgccgaca
     4741 ccgccaccgc cggcatcgga ggccgcgatc gcgttgatcg tgcccggcgc ggacacgccg
     4801 ttcggacgcg caggcgaagc ggtgcgtgaa ggcttcatcg ccgcgcatcg cgccacgcgc
     4861 gctcccgtgg cgatccaggt gatcgagacc gacgccagcg cggcgcaggc cgtggctgcg
     4921 gtgcaggccg cgcgcgaacg cggcgcgcgc ctggtcgtcg gcccgctgac gcgcaacgcg
     4981 gtcaacggcg tggtcgagcg cggcgacgcg ggcatcccga tcgtgctgct gaacgtgccc
     5041 gaccacgccg gcgcgatcgc cgactcgcaa ctggcgttcg gcctgggcgt cgaggacgag
     5101 gcggcgctgc tcacgcgcgc gtcgctgcgc gagatcgacc ccggcgccac cgcgtcggca
     5161 gcgcgccgct gcgtggtcgt ggtcggcgat ggcgcgctgg cgcgtcggat cgcggccgcg
     5221 ttcgtcgccg cgctgaagga cgcgggcgcg ccgaccgtca cgctcgacta cgcgcagcac
     5281 ggcgccgatg gtgtggcgcg cgagatcatg agggcgcccg tcacgtcggt cttcctcgcg
     5341 ctcggcagcc gggacgcgac gcaggtgcgc acgcggctgc cgatcgacgc gccgctgtac
     5401 gcgacctcgc agatcaacgt cggcggcggc atgctgctgg cgaacgacct ggagggcgtg
     5461 cgcttcgtcg acatgccgtg gctgatcgaa ccggagcggc cgacggtgaa ggcgtacccg
     5521 cgcagtgcgg acggctccag caccgagatg cagcgcctgt acgcgctcgg catcgacgcg
     5581 tatcgcgtcg cgctggagtg gctcgcgcac cgcacgcgct tcgagatcga cggcgtgacc
     5641 ggctggctgc gcgtggaccg cgcgcgttcg ccgcgcgtgg aacgaacccc cggctttgcg
     5701 gtctttcggg aaggccgcat cgagcgcagc gacgcgttgc gcgaggtggc gcgctgagac
     5761 aggagcagca tgagttttct gagacggcga ccgggcctgc gcacaccgaa gcagcgcgcc
     5821 ggcgatgcgg ccgagcaggc cgcgtgcgag cggctgctgg cgagcggatg tcgtatcctc
     5881 gcgcgcaacg tgcgctacag ggaaggcgaa ctggacatcg tcgcgcagga cggcgcgacc
     5941 gtcgtgttcg tcgaggttcg actgcgcaac gacgagcgct tcggcggcgc ggccgcgagc
     6001 gtcgatcgtt tcaagcggcg gcggctggcg cgcgccgcgc agcattacct ggtcgagcac
     6061 ttcggcggca gatcgtctgc ggcgctgccg ccatgccggt tcgacgtgat cacggccgac
     6121 gacggcggtg tcggcgaatg gattcgcaac gcgttttcgg aaacgggaga ctgaatgagg
     6181 aagatcctgg tgcgatgggc agcggccggc gcggtcgtgt ccgcagtcgt gggctgcgcg
     6241 ccgctgctcg tcggcggcgt ggtcgctggc ggcgccatgg tggcgaccga tcggcgttcg
     6301 gtcggcacgc agctcgacga cgaggcgatc gagcgtcgcg tcaatcgtgc gctggccgag
     6361 aagttcccgc gcgacagcgc caacatcaac gtcgtcagct acaaccgcaa ggtcctgctg
     6421 acgggcgagg tgacgacgcc gcaggcgaag gacagcgcgg agcagatcgc actgaagacc
     6481 gacaacgtgc gcgaggtgct gaacgaactc gaggtcggag cgctgtcgac gatcggcgcg
     6541 cgcaccgatg acaccgcgac gacggccaag gtgcgcgccg cgctgatcaa cgccaaggaa
     6601 gtgccatccg cggcaataca cgtcgtgacc gagcacggcg tcgtctacct gctcgggcgc
     6661 gtcaccgaga gcgagggcac ctatgccggg accgtcgctg cccgcgtgtc gggcgtgaag
     6721 cgcgtggtca agtccttcga ctacctgacg caggccgaag tgaccgatct gcagtcgaag
     6781 cccgcgcagc cggcgccgac cgacgcgccg cgcaagtagc gcgcgccgcg ctgctgcgcg
     6841 cgaacaggtt cggccgaaaa accgcttgcg cacaagcgcg gttctcttat agtccgccgc
     6901 tcgccgggcg acttggacag ggaggtacgc atgggctggc tgatctttct cggcttgatc
     6961 gcgctgttga tcttctgggc gatcggcgcc tacaaccgtc tggtcggcct gcgcaaccag
     7021 ttcaagaacg cgttcgcgca gatcgacgtg cagctgaagc gccgctacga cctgatcccg
     7081 aacctggtcg agaccgccaa ggcctacatg aagcacgagc gcgagacgct cgaggcggtg
     7141 atcaaggctc gcaacagcgc cgtgaccgcc aaccaggcgg ccgcggccga tcccggcaac
     7201 gcgggcgcga tgcagggcct ggttgccgcg gaaggcgcgc tgcgcggcgc gctcggcaag
     7261 ctcttcgcgc tggccgaggc gtatccggac ctgaaggcca accagaacat gatgcagctc
     7321 accgaggaac tgacaggtac cgagaacaag atcgcgttcg cgcgccaggc gttcaacgac
     7381 gccgtgatgt cgttcaacac cgcggtcgag cagtttccct ccaacgtgat cgccagcgcc
     7441 ttctccttca agccggccga actgcttgct gccaccgaag cgccggaaga gaagaagccg
     7501 gtgaaggtgc agttctagcc gaccctccgg cgtccggcat cgggcgcaaa tgaatttctt
     7561 cgagcatcag gatcaggcgc gccgcaatac caagcggctg gtcgtgctct tcgtgctcgc
     7621 ggtgatcggt gtcgtcattg cggtcaacgt cgtcggcgcg ctcgtctacg tgtcggtgat
     7681 gccgcatccc cgcggcgtgc cgggtgcggc ggcgttgccg aacggattct tcgccgtcaa
     7741 cacgctggtg acgctggcgc tgatcggcgg cggcacgtgg tgggagaccg cgcggctgtc
     7801 cgccggcggc gcggcggtgg ccaagatggt cgacgcgcgg ctgatcgatc cgtcgacgtc
     7861 cgacctgctg gagcgacggc tggtcaacgt cgtcgaggaa atggcgatcg cctccggcat
     7921 tccggtcccg cgcgtgtacg tgatggacca cgagaacacg atcaacgcct tcgcggccgg
     7981 ccactcgatc aacgacgcga tcatcgcggt cacgcgcggc accctgacgc gcttgagccg
     8041 cgacgagttg cagggcgtga tcgggcacga gttctcgcac atcctgaacg gcgacatgcg
     8101 gttgaacctg cggctgatcg gcgtgctgtt cgggctggtg atgatcgcga tgttcggccg
     8161 cttcatgatg cagatgagcc gcggcagccg cgaccgcggc accggcgcgt tcgtgatcgc
     8221 cgggctcgtc ctgtggatcg tcggctacat cggcgtgttg ttcggccgga tcatcaagtc
     8281 cgccgtctcg cgccagcgcg agttcctcgc ggacgcgagc agcgtgcagt tcacgcgcaa
     8341 tcccgacggc atcggcggcg cgttgcgcaa gatcggcggg ctgggcggcg ttggcagcat
     8401 ggcgcccgcc gtcgcggcac ccgacccggc gctgccgcag atggtcgaca ccgcgcacga
     8461 actgcgcgcg cgcggtgtgc gcgtcgacgt cggcagccag atcggccacg cgcacgcgga
     8521 gacgctgtcg cacatgttca tcgcgccggc gcgtctcgcg ttcgccggcg gctggctcgc
     8581 gacgcatccg ccgctcgaag agcgcatcga gcgcatctac ggccgcgcgc tgccgttcct
     8641 gcccgcgccg gagcagccgc tgcagctggc gctggacgcg gcctagcgct ggggccgcag
     8701 tggctgcaag gacctgcccc cgatcgaata cacggtcggc gccgcgtcga ccagcccggt
     8761 cgcgggcctc gtcgctggag gggccgcgtt ggccgacgtc cacgccgcga tcggcgggtc
     8821 gccgccgcag gcgcaggact tcgcgcgccg actgacgcag gagcgcacgg acgcggtcga
     8881 ggcgatcctg accgacacga ccggcgtgca gctgctggtg ctcggcatgc tgatcgagaa
     8941 gggccagccc ttcagcggcc agcagcggca gctcgtgacc gaggcgttcg gcgccacggc
     9001 cgcgcagcgc gtcgacgcgc tgcacgagct gatgcagcag ctgccgccgg gccgccggct
     9061 gccgctgctc gaccgggcga tgccggcgct gcgcaagctg tccatggaaa gcgcggagcg
     9121 gctgctgatg ctttgccacg cgctgatcgc cgccgacggc cgcgtgacgc tgccggaatt
     9181 cctgctgttc acggtgctcg ggcgccgcat cggccgcaac gcacagcgcg cggtgccggt
     9241 caggtaccgc agcgttgccg agcgcgcggc cgacgcggcg ctcgtgctgt cgctgatcgc
     9301 cgcggtgcgc tcgcccgaga aagcggaaca cgcgttcaac gcgggcgcgc tgctgctgcc
     9361 gaacgtcgag gtcgcgcgca ccgatgcggc agcgatccgg ctcgacgaag tctccgccgc
     9421 gctggaacgg ctgaaccagc tcgcgccact ggcgaagccg cagctgatca aggcgtgcac
     9481 tgccgccgcg ttcgtcgacg gccagaccaa ctggaaggcg gccagttgcc tgcgcacgat
     9541 ctgcgccgcg ctcgatgcgc cgctgccgcc gcagatcgat ggcgcgccgg aggaggacac
     9601 gccggccgcg gcggtcgcgc agccggtgca gggcgaattg ctgtgatcca gcgcgctacc
     9661 tgattgcgcc gaacaccttc ttcagtattt cgctgcccgt gccgaccggg tcctggcgga
     9721 tcttcttctc ttcttcgccg atcatgaagt acatgccgtc gagcgccttc gcggtcacgt
     9781 ggcgctcgat ccgggactga tcgccgcgca cgagcccgag ctgctcgcct ttcgctgcca
     9841 gcgtgttgta ctgtcgcgcc aggccgatgc gatcggtcac cttggtcacg atcggcagga
     9901 acttgccgct cagcggctcg cgcgtcttcg cggcgaagaa cttggtcacc gcgtcgttgc
     9961 cgccggtgag gatgcccttg gcgtcctgca cggtcatgtt gcgcactgcg ctcaccagca
    10021 acgccttggc ctgcggcact gcctgctcgg ccgcgcgatt gacgccgagc ttcagatcgt
    10081 cgatgtcctt gccacggccg acaagcttca gcgcaccctc ggcgcgctga agccattcgg
    10141 gcagcgggat gcgcaccttg tcgttgcccc agaagccgtc ggtgcggccg agcagttcga
    10201 ccgcgaccgt cgcgcccttc tcgagcgctg ccttcacgcc cgcgctcgcg tccttctgcg
    10261 tcaggtccgc cagcgacagc gcgaacgcgg cgcggctgcc gagcgccgcc gccaatgcgc
    10321 ttcctatgac cagtgcgcgc cgcacctcgt cggcgctgaa ttcatgctcg agcatcctgt
    10381 tctcctgctg cagcggcacc gcgtcgccgc gttgatcgtg gtcctgcttg cgatgggagc
    10441 agtgttcacc gcggcggcag tgctgtcaaa accgcgcgca cctgacctgg cgccggtcaa
    10501 tctcgacggc gaacgcgagc gctggcggat ctgcgcgggc aggtggtgcg ggtgaatttc
    10561 cgggcgacga cccgcgtggt ctgcgtgaag cagatgccgc agcgcgcgga caccgatcgc
    10621 aggttcgcgg ggcgcggcct gcgcgcttcg cggtggcgat gccgtgcgac cggccggatc
    10681 gagtgctcgg caaccgcgag cgcaaccggc tgccgtttcc gatcgcgctc gacgtgcagg
    10741 gcgagatcgc gcgcgcgttc gacgccgcgc gcgtgacgcc gaccacgttc ctgatcgaca
    10801 agcggggacg gatcgttaag cgctacatcg gtgagccgga cctcaccgaa atgcgagagc
    10861 ggatcgagaa accgcgagcc ggctaaacga cgcctgcctt gtgcgcttgc tggtcggcgt
    10921 agtacgagct gcgcaccagc ggcccgcacg ctgcgtgcga gaagcccagc gccaccgcct
    10981 cgcgctcgaa catcgcgaac gtgtccggat gcacgtagcg ctgcaccggg aaatgcgcgg
    11041 tgctcggcgc gagatactgg ccaagcgtca gcatgtccac gtcgtgcgcc cgcaggtcgc
    11101 gcagcacctg caggatctcg tcgtcgttct cgcccaggcc cagcatcagc ccgctcttgg
    11161 tcggcacgct cggcacgcgg cgcttgaatt cctgcagcag cttcagcgaa tgcgcgaagt
    11221 ccgatcccgg ccgcgccacc ttgtacaggc gcggcacggt ctcgagattg tgattcatca
    11281 cgtccggcgg cgcggcttcg aggatctcca gggcgcggtc gagccgcccg cggaagtcgg
    11341 gcaccagcac ctcgatcttc gtcgccggcg accgctcgcg caccgcgcgg atgcagtcga
    11401 cgaagtgctg cgcgccgccg tcgcgcaggt cgtcgcggtc gaccgacgtg atcacgacgt
    11461 agctcaactt cagcgcggcg atcgtctgcg ccaggttgat cggctcgtcg gcatcgagcg
    11521 gctcgggccg gccgtgcgcg acgtcgcaga acgtgcagcg gcgcgtgcac ttgtcgccca
    11581 tgatcatgaa ggtcgccgtg cccttgccga agcactcgcc gatgttcggg cacgacgctt
    11641 cctcgcacac cgtgtgcagc ttgtggctgc gcaggatgtc cttgatctcg tagaagcgcg
    11701 tggtcggcga gccggcgcgc acgcggatcc agtcgggctt gcgcagcgtc gtgcgtgctt
    11761 cgaccgcgat cggaatcttc ttgaacacga cgccggtctt cgcgtcgccc ttcttcttcg
    11821 cgaaaggatc gggggaggta ctcatcggga gaagtggctg gcgagccgct cgctcaggcg
    11881 ctgcgcggcg tcggcccacg tggtcgacac accgattcta cgcaggtcga ccgtggccag
    11941 acccgcatag ccgcacggat cgatgcggga aaacggcgcg aggtccatcg cgacgttgaa
    12001 cgccacgccg tggtagctgc agccgcgcgt gatcttgatg ccgatcgcgg cgatcttggc
    12061 gagtcctgcg aattcgccgc tcgatgtcga ctcgaacggc acgtagatgc ccggcgcgcc
    12121 cttgacgcgc tggccggcga tcccgtagct gtcgagcacc tggatcacgc tttgctcgat
    12181 gcggaacacc agttcccgaa cgaagatccc gagccgcttc agatccagca gcggatacgc
    12241 gaccacctgc cccggaccgt ggtacgtgac ctggccgccg cgatcggtcg cgatcaccgg
    12301 gatgtcgccg gagtcgagca cgtgctcgcg ccggccggcc tgcccgagcg tgaacacggg
    12361 cgggtgctcg acgatccaca gttcgtccgg catgtcggcg gtgcgccgcg cggtgaactc
    12421 gcgcatggcc tgccacgttg ccaggtagtc ggcgcggcct aacgagcgca cgaccagcgc
    12481 ccgcactgcg ggcgcaggcg cgctgtcgat caaagcacca ccttgaccag cgggtgcgag
    12541 gtcagcgcgc gatacaggtc gtcgagctgc tcgcgcgagg tcgcgttgac ggtggcagtc
    12601 accgagaggt agttgcccgc cttcgagacg cgcaattcga gcgtcgcagg gtcgaagtcc
    12661 ggcgcgtgct tctgcacgac gtcgaccacg gcctgcgcaa agccattgac acgcttgccc
    12721 atcaccttga tggggaaatc ggtcgggaaa gaaagcgatg attcggtctg catttcgccg
    12781 atatggggac gcgcgccgga ttttctagct ggcgcggttc agccgctgca tttcggcg
//
LOCUS       DBNHBY010000172         3803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_888970, whole genome shotgun sequence.
ACCESSION   DBNHBY010000172 DBNHBY010000000
VERSION     DBNHBY010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_888970"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="AC16HB_04225"
     CDS             <1..749
                     /locus_tag="AC16HB_04225"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HIR5606029.1"
                     /translation="PSRLISGVADVAFVDGTLYALLAGAGCSHGASMPNGVVRVNSND
                     TWSLIANLSAYQQANPTKVIEPDDFEPDGTWYGMVAVRGALYAVEPNHGEMVKITTDG
                     KITRVIDISERLGHEVPTAISYHGVFYVGNLGRFDPDDVDQQKIVQITPSGQLRVVAT
                     GLSKVLGVAFDGRDRLYALEMSKGDAGPVPFTSRLVRVTRNGDRKVLVDGLFFPTGMT
                     FGPDGALYVSNAGFGPPVGEILRIEVPDAD"
     gene            complement(782..1531)
                     /locus_tag="AC16HB_04230"
     CDS             complement(782..1531)
                     /locus_tag="AC16HB_04230"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR5606030.1"
                     /translation="MDPSNSTPVEPRSVDAGRGISWWSEAWALFMKNAGVWIVIALIM
                     IVIFIALHFIPILGSIAGSLILPVFTAGWLLAARKVEGGGTLEVGDLFAGFRDKLTPL
                     LVLGALLLAAGFVIALAAGALGFGAVMGMFMGGAQHSTGGMMAAFGAGMLAVLVALAL
                     GALVAMALWFAPALVVFRDVAPVDAMKQSFAASLKNIVPFLLYGIAYLIAAIVASIPF
                     GLGWIVLLPVLMLTAYTSYQDVFGTSSAEHV"
     gene            complement(1555..2334)
                     /locus_tag="AC16HB_04235"
     CDS             complement(1555..2334)
                     /locus_tag="AC16HB_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5606031.1"
                     /translation="MTEQAKSEQHAHHGHPIISFDKVNKWYGNNFHVLRDINLQVARG
                     ERIVICGPSGSGKSTLIRCINRLEEHQEGHILVDGTELTDDVRAIEKVRKEVGMVFQQ
                     FNLFPHLTVIENLTLAPMWVGKLPKKEAEERAMAQLKRVRIAEQALKYPLQLSGGQQQ
                     RVAIARALCLTPKIMLFDEPTSALDPEMIKEVLDVMIELAKGGITMLCVTHEMGFAKA
                     VANRVIFMDQGQIVEENSPHEFFNNPKSDRTKDFLSKILGH"
     gene            complement(2348..3451)
                     /locus_tag="AC16HB_04240"
     CDS             complement(2348..3451)
                     /locus_tag="AC16HB_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5606032.1"
                     /translation="MAAIFQPVAPRPAPVKLEGFVPWVKSNLFSNWRNTLITVLLGAL
                     IVYWAPSIINWTLLQAVFEPNADVCQSARGTGACWGVVAEKYRLIIFGRYPYEQQWRP
                     EVATLLLLAMLVTSCIRAFWKPWLGAVWIVGLPSFFILMYGGLLGLEVVQTDRWGGLP
                     LTIMLASLSIILAFPLAVLVALGRRSNLPAIRTTCVIYVELIRGVPLISVLFMASFMF
                     PLFMPPGLTIDVLLRVLVGITLFAAAYLAEIVRGGLQAIPKGQIEAAESLGLSYWQTQ
                     RKIVLPQALALVVPGIVNSFISIFKDTSLVAIVSLYDLTGSLGLALNSDADWRPFKIE
                     GYLFITGIYFVFCFAMSRYSLWIEKQVNVSKTR"
     gene            complement(3454..>3803)
                     /locus_tag="AC16HB_04245"
     CDS             complement(3454..>3803)
                     /locus_tag="AC16HB_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5606033.1"
                     /translation="EVVRAGIASVAHGQVEAASSLGLSRGHSMRLVILPQALRVIIPP
                     ITNQYLNLTKNSSLAVAIGYPDVVSISNTSLNQTGRAVECIAIIMLVYLTTSLTTSGI
                     MNWYNKRSAIKER"
BASE COUNT          711 a         1277 c         1196 g          619 t
ORIGIN      
        1 taccgtcacg gctcatcagc ggcgtggccg atgtcgcctt cgtcgacggc acgctgtacg
       61 cactgctcgc cggcgcgggt tgctcgcacg gcgccagcat gccgaacggc gtggtgcgcg
      121 tgaactccaa cgacacatgg agcctgatcg ccaacctgag cgcgtaccag caggcgaatc
      181 cgaccaaagt catcgagccc gacgatttcg agcccgacgg cacttggtac ggaatggtcg
      241 cggtgcgcgg cgccctgtac gcagtcgagc cgaatcacgg cgagatggtg aagatcacga
      301 ccgacggcaa gatcacgcgc gtgatcgaca tttccgaacg gctgggccac gaggtcccca
      361 ccgcgatctc ctatcacggc gtgttctacg tcgggaacct cggccgcttc gacccggacg
      421 acgtcgacca gcagaagatc gtgcagatca cgccgagcgg gcaactgcgc gtcgtcgcga
      481 caggcctgag caaggtgctg ggcgtcgcat ttgacggccg ggaccggcta tatgcgctcg
      541 aaatgtcgaa gggagatgcc ggcccggtcc cgttcaccag tcgcctcgtt cgcgtcacgc
      601 gcaacggcga ccggaaggtg ctggtcgatg ggctgttctt cccgaccggg atgacgttcg
      661 gcccggacgg cgcgctgtat gtctcgaatg ccggcttcgg cccgccggtc ggcgagatcc
      721 tgcgcatcga agtgccggac gccgattagg cggcacggtt cgtgacggcc gtgccgatgc
      781 gtcagacgtg ttcggcgctc gacgtgccga acacgtcctg gtacgacgta taggccgtga
      841 gcatcagcac cggcagcagc acgatccagc cgagcccgaa cggaatcgac gccacgatcg
      901 ccgcaatcaa gtaggcgatg ccgtacagca ggaacggcac gatgttcttc agggatgcag
      961 cgaaactctg cttcatcgca tcgaccggcg ccacgtcgcg gaacacgacc agcgccggcg
     1021 cgaaccacag cgccatcgcg accagggcgc cgagcgccag cgccaccagt actgcaagca
     1081 tgccggcgcc gaaggcggcc atcatgccgc cggtgctgtg ctgcgcgccg cccatgaaca
     1141 tgcccatcac cgcaccgaag ccgagcgcgc cggcggcgag cgcaatgacg aagcccgctg
     1201 ccagcagcag cgcgccgagc acgagcagcg gcgtcagctt gtcgcggaag ccggcaaaca
     1261 ggtcgcccac ttcgagcgtg ccgccgccct cgaccttgcg cgccgcaagg agccaaccgg
     1321 cggtgaacac cggcaggatc agcgagccgg caatgctgcc gaggatcggt atgaagtgca
     1381 gcgcgatgaa gatcacgatc atgatgagcg ctatcacgat ccacacgccg gcattcttca
     1441 tgaagagcgc ccacgcctcg ctccaccacg agatgccgcg tccggcatcg acgctccgcg
     1501 gctcaacggg cgtcgagttc gatgggtcca tcgggccctc cccttctacg tgcctcagtg
     1561 cccgaggatc ttcgacagga agtccttggt gcggtcgctc ttcggattgt tgaagaactc
     1621 gtgcgggctg ttctcctcga cgatctggcc ctggtccatg aagatcaccc ggttggccac
     1681 cgccttggcg aagcccatct cgtgcgtcac gcacagcatc gtgatgccgc ccttggccag
     1741 ctcgatcatc acgtcgagca cttccttgat catctcgggg tcgagcgcgg aggtcggctc
     1801 gtcgaacagc atgatcttcg gcgtcaggca cagcgcccgc gcgatcgcca cgcgctgctg
     1861 ctggccgccc gacaactgca gcgggtactt gagcgcctgc tcggcgatgc gcacgcgctt
     1921 cagttgcgcc atggcgcgct cctcggcttc cttcttcggc agcttgccga cccacatcgg
     1981 cgccagcgtc aggttctcga tcaccgtcag gtgcgggaac aggttgaact gctggaacac
     2041 catgccgact tccttgcgca ccttctcgat cgcgcgcacg tcgtcggtga gctccgtgcc
     2101 gtcgacgagg atgtggccct cctggtgctc ctccagccgg ttgatgcagc ggatcagcgt
     2161 cgacttgccc gaccccgacg ggccgcagat cacgatccgc tcgccgcgcg cgacctgcag
     2221 gttgatgtcg cgcagcacgt ggaagttgtt gccgtaccac ttgttcacct tgtcgaagga
     2281 gatgatcggg tggccgtgat gggcgtgttg ttcggacttc gcctgctcgg tcatggtggc
     2341 tccggactca gcgcgtcttg ctgacgttga cctgcttttc gatccacagg ctgtagcgcg
     2401 acatcgcgaa acagaacacg aagtagatcc ccgtgatgaa cagataccct tcgatcttga
     2461 acggccgcca gtcggcgtcg gagttcagcg ccaggccgag cgatcccgtc aggtcgtaca
     2521 gcgaaacgat cgccaccagc gacgtgtcct tgaagatcga gatgaagctg ttgacgatgc
     2581 ccggcacgac cagcgccagc gcctgcggca gcacgatctt gcgctgcgtc tgccagtacg
     2641 acagcccgag cgactcggcc gcctcaatct ggcccttcgg gatcgcctgc agcccgccgc
     2701 gcacgatctc ggccagatac gccgccgcga acagcgtgat gccgaccagc acgcgcagca
     2761 gcacgtcgat cgtgagtccc ggcggcatga acaacgggaa catgaagctc gccatgaaaa
     2821 gcaccgagat cagcggcacg ccccggatga gttccacgta gatcacgcag gtggtacgga
     2881 tcgccggcag gtttgaacgc cgaccgagcg cgaccaggac cgccagcgga aacgccagga
     2941 tgatcgacag cgacgccagc atgatcgtca acggcaggcc accccagcga tcggtctgca
     3001 cgacctcgag tcccagcaat ccgccgtaca tcaggatgaa gaacgatggc agcccgacga
     3061 tccacacggc accgagccac ggcttccaga acgcgcgaat gcagctcgtc acgagcatcg
     3121 ccagcagcag cagcgtcgcg acctcgggcc gccattgctg ctcgtacgga tagcgtccga
     3181 agatgatcag ccggtacttc tccgcgacca caccccagca ggcgccagtg ccacgcgcgc
     3241 tctggcacac gtcggcgttc ggttcgaaca ccgcctgcag cagcgtccag ttgatgatcg
     3301 acggcgccca gtagacgatc agcgcgccga gcagcaccgt gatcagggtg ttgcgccagt
     3361 tgctgaacag gttgctcttg acccagggaa cgaagccctc gagcttcacc ggcgccgggc
     3421 gcggtgcgac gggctggaat atcgcagcca tcgtcaccgc tccttgatcg ccgagcgttt
     3481 gttgtaccag ttcatgatcc ccgacgttgt gagcgacgtg gtcaggtaca ccagcatgat
     3541 gatcgcgatg cactcgaccg cgcgcccggt ctggttgagc gaggtgttgg agatcgacac
     3601 cacgtccgga tagccgatcg ccacggcaag cgacgagttc ttggtcaggt tcagatactg
     3661 gttggtgatc ggggggatga tcacgcgcag cgcctgcggc aggatcacca gccgcatcga
     3721 atgcccgcgc gacagcccga gcgaactggc ggcctcgact tgaccgtgcg ccactgacgc
     3781 gatgccggcg cgcacgactt cag
//
LOCUS       DBNHBY010000173         2291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1092679, whole genome shotgun sequence.
ACCESSION   DBNHBY010000173 DBNHBY010000000
VERSION     DBNHBY010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1092679"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="AC16HB_04250"
     CDS             <1..2073
                     /locus_tag="AC16HB_04250"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR5606034.1"
                     /translation="PAQAGLKKPPAAVDGARLVAADREPGNWMSPGRTYEEQRFSPLK
                     QVNDGNVDQLGLAWTFKYDLDRVIEATPIVVDGVMYTTSAYSMVYALDAATGKLLWKY
                     DPKVFRAIQATGCCDAANRGVAVWQGKVYVGVYDGRLEALDAKTGAKVWSVDTVIDHE
                     RNYTITGAPRIVKGKVIIGNGGSEFGVRGYVTAYDAQTGKQLWRFFTVPGDPAKGDEN
                     AAMTMARKTWFGDKYWKYGGGGTVWDSMAYDPALDLLYLGVGNGSPWNYGARSEGKGD
                     NLFLSSIVAVRPDTGEYVWHYQTTPGEAWDYTATQSIIVATLPINGKPRKVVMQAPKN
                     GYFYVLDAATGEFLSAGQFATKLTWAKGIDPKTGRPIIDLEAADYWTKGEGKMVFPGS
                     QGAHNWFPMSYSPLTGLVYIPQHTTAEVYVPLKDPPMPRKGWPGLGVEMPRIPEDKAG
                     IDELAKIFTGKLQAWDPVQQKEIWSAPYPIMSNGGTLATAGNLVFQGTVDGRLIAFTA
                     DKGKEVWQREVSSAAVAGPITYEVNGEQYVTFNVGWGGSFPITFGALAERARTVPDSR
                     IMTFKLGGKAPMPEVKRRVLTPPNPPPVTADAATLEEGRTLFNNRCGACHGFGARSAN
                     VIPDLRYLTPEKHEQFMGFVYGMAAERGMPNFVGILEPADVAKIHQYLIQRSHDLKNE
                     LAQAATKP"
     gene            2070..>2291
                     /locus_tag="AC16HB_04255"
     CDS             2070..>2291
                     /locus_tag="AC16HB_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5606035.1"
                     /translation="MKALLCKAYGDPSALVYEEAPDPVPGPGEVLIAVRAAGVNFPDA
                     LMVQGKYQFKPALPFAPGAELAGDVIALGE"
BASE COUNT          422 a          742 c          795 g          332 t
ORIGIN      
        1 ccggcacagg cagggttgaa gaagccgccg gcggcggtcg atggtgcccg gctggtcgcc
       61 gcggaccgtg agcccggcaa ctggatgagc cccggcagga cgtacgaaga gcagcgcttc
      121 agcccgctca agcaggtcaa cgacggcaac gtggaccagc tcgggctggc gtggacgttc
      181 aagtacgacc tcgaccgcgt gatcgaagcc acgccgatcg tcgtcgacgg cgtgatgtac
      241 acgaccagcg cgtacagcat ggtttacgcg ctcgacgccg cgacgggcaa gctgctgtgg
      301 aagtacgacc cgaaggtgtt ccgtgccatc caggccaccg gttgctgcga cgcggccaat
      361 cgcggcgtag ccgtctggca gggcaaggtg tacgtcgggg tgtacgacgg ccgcctcgag
      421 gcgctggacg ctaagaccgg cgccaaggtc tggtcggtcg acaccgtcat cgaccacgag
      481 cgcaactaca cgatcacggg cgcgccgcgc atcgtcaaag gcaaggtcat catcggcaac
      541 ggcggcagcg agttcggcgt gcgcggctac gtcacggcct acgacgcgca gaccggcaag
      601 cagctgtggc gcttcttcac cgtgcccggc gaccctgcga agggcgacga gaacgcggcc
      661 atgaccatgg cgcgcaagac ctggttcggc gacaagtact ggaagtacgg cggcggcgga
      721 acggtgtggg attcgatggc ctacgatccc gcgctcgatc tcctgtacct gggcgtcggc
      781 aacggctcgc cctggaatta cggtgcgcgg tcggaaggca agggcgacaa cctgttcctc
      841 tcgtccatcg tcgctgtcag gccggacacc ggcgagtacg tctggcatta ccagacgact
      901 cccggcgagg cctgggacta cacggcgacg cagagcatca tcgtcgcgac gctcccgatc
      961 aacggcaagc cgcgcaaggt cgtgatgcag gcgccgaaga acggttactt ctacgtgctc
     1021 gacgcagcga cgggcgagtt cctctcggcg gggcagttcg ccaccaagct cacgtgggcc
     1081 aagggcatcg acccgaagac cggccggccg atcatcgacc tcgaggcggc ggattactgg
     1141 accaagggcg aagggaagat ggtcttcccg ggctcgcagg gcgcgcacaa ctggttcccg
     1201 atgagctaca gcccgttgac cgggctggtc tatatcccgc agcacaccac cgccgaggtg
     1261 tacgtgccgc tgaaggatcc gccgatgccg cgcaagggct ggcccggtct aggcgtcgag
     1321 atgccgcgca tccctgaaga caaggcgggc atcgacgagc tggcgaagat cttcaccggc
     1381 aagctgcagg cgtgggatcc cgtgcagcag aaggaaatct ggtcggctcc gtatccgatc
     1441 atgtccaacg gcggcacgct ggccacggcc ggcaacctcg ttttccaggg caccgtcgac
     1501 ggccgactga tcgcgttcac ggcggacaag ggcaaggaag tctggcagcg cgaggtgagc
     1561 agcgccgccg tcgccggacc gatcacctac gaggtgaacg gcgagcagta cgtcacgttc
     1621 aacgtcggct ggggcggctc gtttccgatc acgttcggcg cgctggccga gcgcgctcgc
     1681 acggtgccgg attcgcgcat catgacgttc aagctcggcg gcaaggcgcc gatgcccgag
     1741 gtcaagcggc gcgtgctgac gccaccgaac ccgccgccgg tcacggccga tgcggccacc
     1801 ctcgaagagg ggcgcaccct gttcaacaac cgctgcggcg cctgccacgg cttcggcgcg
     1861 cgcagcgcca acgtgatccc cgacctgcgt tacctgacgc cggagaagca cgagcagttc
     1921 atgggcttcg tgtacggcat ggcggcggaa cgcgggatgc cgaatttcgt cggcatcctc
     1981 gagccggcgg acgtggcgaa gatccaccag tacttgatcc agcgcagcca cgacctgaag
     2041 aacgaactgg cgcaggcggc gacgaaacca tgaaggcgct gctgtgcaag gcgtacggcg
     2101 acccgagcgc gctggtctac gaagaagcgc ccgatccggt gccgggcccc ggcgaggtcc
     2161 tgatcgccgt gcgtgcggcc ggggtcaatt tcccggacgc gctgatggtg cagggcaagt
     2221 accagttcaa gccggcgctt ccctttgcgc cgggggctga actggcgggc gacgtcatcg
     2281 cgctgggtga g
//
LOCUS       DBNHBY010000174         4626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1498485, whole genome shotgun sequence.
ACCESSION   DBNHBY010000174 DBNHBY010000000
VERSION     DBNHBY010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1498485"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..504
                     /gene="ribH"
                     /locus_tag="AC16HB_04260"
     CDS             13..504
                     /gene="ribH"
                     /locus_tag="AC16HB_04260"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5606036.1"
                     /translation="MAIDFIAPDLDGSELRIGLVQARFNGWAGDALAKSCLDELRALG
                     VDEDDITHVTVPGALEVPLVLAKLAEAGDFDALIAIGCVIRGETYHFEIVANESARGI
                     AQVALERGVPIANAILTTDNEEQARVRVAEKGRDAARVAVEMANLMWALDEGGESDSA
                     DNE"
     gene            482..961
                     /gene="nusB"
                     /locus_tag="AC16HB_04265"
     CDS             482..961
                     /gene="nusB"
                     /locus_tag="AC16HB_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5606037.1"
                     /translation="MTARTTSSPVVSAPGGRGGRSARRRARELALQGLYQWLIGGQNL
                     AAIEVHLMETEGYDRADAAHLREVLQGAIEGADDLRAEFGGFLDRSVAELSPIEHAIL
                     LIGSYELKHRAEIPFRVVINEAVELAKSFGGTEGFKYVNGVLDKLALRLRPNEVGRH"
     gene            1005..1976
                     /gene="thiL"
                     /locus_tag="AC16HB_04270"
     CDS             1005..1976
                     /gene="thiL"
                     /locus_tag="AC16HB_04270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR5606038.1"
                     /translation="MNEFELIARYFTRPSRERQGVGDDCALIDVGDRTLAITCDMLVE
                     RRHFFPGVDPEGLGHKALAVNLSDLAAAGAAPRCFLLALALPRADESWLAAFARGLFG
                     LADQFGCELIGGDTTRAPQWHQTAGPLTICITALGEVVSGRALSRAGARPGDDLWVSG
                     TLGDAALALALADGTASAEPADRRHCEARLERPMPRVRLGERLRGLATAAIDVSDGLL
                     GDLAHIAERSRVGARLHWDEIPRSPALQGQPAALQQRCALAGGDDYELLFTAPPSHRL
                     QVVAAARDGGVAVARIGEILAAPGIAVLDAASANMETPFSAFDHFRS"
     gene            1994..2566
                     /locus_tag="AC16HB_04275"
     CDS             1994..2566
                     /locus_tag="AC16HB_04275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR5606039.1"
                     /translation="MATMPLVNDPQPPQVLAAVKGESAPPRERPARPTWRWMWSHPWY
                     VLALACGAGLPRFAPGTLGTLVAWALFALLDPVLTDLSRAGFIVAGLYFGAWAAQRAG
                     AALGSSDSGHIVIDEVVAFWIVLWLLPPHGLWLQALAFILFRLFDIAKPPPIRRLEAR
                     FRNGFGVMLDDLVAAFYTLLAIAIGVRVLG"
     gene            2563..3087
                     /locus_tag="AC16HB_04280"
     CDS             2563..3087
                     /locus_tag="AC16HB_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR5606040.1"
                     /translation="MNDVSDHIVGLSRDLGALLLAQHLMVATAESCTAGGIAYAITQT
                     PGSSQWFDRGFVTYSNPSKQTVLGVSTAYLRDFGAVSEPVARAMALGALTHSQAQITV
                     AVTGIAGPGGGTPDKPVGRVCLAWAIKRDPSSAPWVRTATRSLGGDRAAVRTQAIIEA
                     LEGLIGLLKQRQDV"
     gene            3091..3981
                     /locus_tag="AC16HB_04285"
     CDS             3091..3981
                     /locus_tag="AC16HB_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5606041.1"
                     /translation="MEWFPGFSVDRRAVNGVRIHVRHTVGQSSRPPMLLLHGFPQTHA
                     MWHKVAPRLASHFTLVLPDLRGYGDSDKPGGPADHSAYSKRAMAADQVALMRSFGFES
                     FHVCGHDRGARVAHRLALDHPQAVRRLMLLDISPTLTMYERTTMEFARLYYHWFFLIQ
                     PEPLPERLIGADPVFYLRRKIGAWGSGATDFFDPQALAEYERCFANPAAIHAMCEDYR
                     AAATIDLEHDQADAERRIQCQLRVLWGEHGVVHRLFTPIADWQAKARGTVTGRATPSG
                     HYITEQVPDLLLDDMLEFFR"
     gene            3978..4328
                     /locus_tag="AC16HB_04290"
     CDS             3978..4328
                     /locus_tag="AC16HB_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606042.1"
                     /translation="MIDAPFHRALRLGLHGFPHPEAEEEVAQMVRGIWTPHAPWIVSN
                     EAPVDALLLWRGTRTDDPVDAAVLRVNLHRSGSSRRDADRRLEPLFVRRPLRQSALRV
                     ALDAAFARIQRLQK"
     gene            complement(4331..>4626)
                     /locus_tag="AC16HB_04295"
     CDS             complement(4331..>4626)
                     /locus_tag="AC16HB_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606043.1"
                     /translation="ANIVVDCSHANSMKEPSLQPLVFMDCVHQIKEGNRSIVGLMLES
                     SLNAGNQPIPDDLSQLAYGVSVTDACIGWDTTRELLQRAHGELAPTLAQRARS"
BASE COUNT          657 a         1577 c         1641 g          751 t
ORIGIN      
        1 ggaagggtga gcgtggcgat cgatttcatc gcgccggatc tcgatggttc ggaactgcgg
       61 atcgggctgg tgcaggcgcg cttcaacggc tgggccggcg atgcgctggc caagtcatgc
      121 ctcgacgaac tgcgggcact cggcgtcgac gaggacgaca tcacgcacgt gacggtgccg
      181 ggcgcgctcg aagtgccgct cgtgctggcg aagctcgcgg aggcgggcga cttcgacgcg
      241 ctgatcgcga tcggctgcgt gatccgcggc gaaacgtacc atttcgagat cgtcgccaac
      301 gagtcggccc ggggcattgc gcaggttgcg ctggaacgcg gcgtgccgat cgccaacgcc
      361 attctcacca ccgacaacga ggagcaggcg cgggtgcgtg tggccgaaaa gggccgcgac
      421 gccgcccgcg tcgcggtcga gatggcgaac ctgatgtggg cgctggacga aggcggcgag
      481 agtgacagcg cggacaacga gtagcccggt cgtctctgcg ccaggcggcc gcggcggccg
      541 cagcgcgcgg cggcgtgcac gcgaactggc cctgcagggc ctgtaccagt ggctgatcgg
      601 cggccagaac ctggccgcga tcgaggtcca tttgatggag acagagggct acgaccgtgc
      661 cgacgccgcg cacctgcgcg aagtgctgca gggtgcgatc gaaggcgccg atgacctgcg
      721 cgccgaattt ggcggctttc tcgatcgcag tgtggcggaa ctgtcgccta tcgagcacgc
      781 gatcctgctg atcggcagct acgaactgaa gcatcgggcc gaaatcccgt tccgcgtggt
      841 gatcaacgag gcggtcgaac tcgccaagag cttcggcggc accgaaggct tcaagtacgt
      901 gaacggcgtc ctcgacaagc tggcgctgcg gctgcggccg aacgaggtgg gccggcactg
      961 aacgacccgc ggctactggc gtccgctccc gttcgccgat ctcgatgaac gagttcgaac
     1021 tgatcgcccg ctacttcacg cgtccatcgc gcgagcggca gggcgtcggg gacgactgcg
     1081 ccctgatcga tgtcggcgac cgcacgctgg caatcacctg cgacatgctg gtcgagcggc
     1141 gccatttctt ccccggcgtc gaccccgagg ggctcggcca caaggcgctg gcggtgaacc
     1201 tctccgatct ggccgcggcc ggggcggcgc cgcgctgctt cctgcttgcg ctcgccctgc
     1261 cgcgcgcgga tgaaagctgg ctcgctgcgt tcgcgcgcgg gctgttcggt ctggccgacc
     1321 aattcggctg cgaactgatc ggcggcgata ccacccgggc gccgcagtgg caccagaccg
     1381 ccggcccgct gacgatctgc atcaccgcgc tgggcgaggt cgtgtccgga agggcgctgt
     1441 cgcgcgccgg cgctcgcccg ggcgatgacc tctgggtgtc cggaacgctc ggcgacgccg
     1501 cgctggcgct ggcgctggcg gacggcacgg ccagtgcgga gcccgccgac cgacgacact
     1561 gcgaggcgcg gctggagcgc ccgatgcccc gggtacgcct gggcgagcgg ctgcgcgggt
     1621 tggcgactgc ggccatcgac gtgtcggacg ggttgctcgg tgacctggcg cacatagccg
     1681 agcgatctag ggtcggggcc cggttgcatt gggatgagat accgcgctcg ccggcactgc
     1741 aaggccagcc ggcggcgttg cagcagcgct gcgcgctggc gggcggcgat gactacgagc
     1801 ttctcttcac ggccccgccg tcgcaccggc tgcaggtcgt cgcggcggct cgggatggcg
     1861 gggttgcggt cgcccgaatc ggcgagatac tggccgcgcc cggcatcgcg gtgctcgacg
     1921 cggcgagcgc gaacatggaa actccgttct cggcgttcga ccatttccgc tcctgacgtc
     1981 gtctgccgcc gccatggcga ccatgcccct cgtcaacgat ccccagccgc cgcaggtgct
     2041 tgctgccgtt aaaggcgaat cggctccgcc gcgcgagcgg ccggcacgcc cgacctggcg
     2101 ctggatgtgg tcgcacccct ggtatgtgct tgcgctggct tgcggtgccg ggttgccgcg
     2161 cttcgcgccc ggcaccctgg gcacgctggt tgcatgggcg ctgttcgcgc tgctcgatcc
     2221 ggtgctgacc gacctgtccc gggccggctt catcgtcgcg ggcctgtact tcggcgcctg
     2281 ggccgcgcag cgcgccggcg ccgcgctcgg cagttccgac agcggccaca tcgtcatcga
     2341 cgaggtggtc gcgttctgga tcgtgctgtg gctgctgccg ccgcatgggc tgtggctgca
     2401 ggcgctggcg ttcatcctat tccgcctctt cgacatcgcc aagccgccgc cgatccgccg
     2461 cctggaagcg cgcttccgaa acggcttcgg cgtgatgctc gacgatctcg tcgcggcctt
     2521 ctacacgctg ctcgcgatcg cgatcggtgt gcgggtgctc ggatgaacga cgtctccgat
     2581 cacatcgtcg ggctgtcgcg ggatctcggc gcgctgctgc tggcgcagca cctgatggtg
     2641 gcgactgccg agtcgtgcac cgccggcggc atcgcatatg cgatcacgca gactccgggc
     2701 tcgagccaat ggttcgaccg cggcttcgtg acgtattcca acccgtcgaa acagaccgtc
     2761 ctcggggttt cgaccgcgta cctgcgcgac ttcggcgcgg tgagcgagcc ggtggcgcgc
     2821 gcgatggcgc tcggtgcact gacacacagc caggcgcaga tcacggtggc ggtcaccggc
     2881 atcgcgggtc ccggcggtgg cacgcctgac aagccggtcg gcagggtgtg cctcgcttgg
     2941 gcgatcaagc gcgatccgtc cagcgcaccg tgggtgcgca cggccacgcg cagtcttggc
     3001 ggcgatcgcg cggccgtgcg cacccaggcg atcatcgaag cactcgaagg gctgatcggc
     3061 cttctcaagc agcgacagga cgtctgacgg atggagtggt tccccggctt ctcggtggac
     3121 cggcgcgccg tcaacggcgt gcggatccac gtccgccaca cggtcggcca gtcgagccgg
     3181 ccgccgatgc tgctgctgca cggctttccg cagacccacg cgatgtggca caaagtggcg
     3241 ccgcggctcg cttcacactt cacgctggtt ctgcccgatc tgcgcggcta cggtgactcg
     3301 gacaaaccgg gcggcccggc cgatcactcg gcgtacagca agcgcgcgat ggccgccgac
     3361 caggtcgcgc tgatgcgcag cttcggcttc gagtccttcc acgtctgcgg tcacgatcgc
     3421 ggcgcgcgcg tggcgcaccg gctggcgctg gaccatccgc aggccgtccg ccggctgatg
     3481 ctgctcgaca tctcgccgac actgacgatg tacgagcgca cgacgatgga gttcgcgcgg
     3541 ctctactacc actggttctt cctgatccag ccggagccgc tgcccgagcg actgatcggc
     3601 gcggacccgg tgttctacct gcgccggaag atcggcgcct gggggtccgg cgcgaccgac
     3661 ttcttcgatc cgcaggcgct ggccgagtac gaacgctgct tcgcgaaccc cgccgcgatc
     3721 cacgcgatgt gcgaggacta ccgcgcggcc gcgacgatcg atctcgagca cgatcaggcc
     3781 gacgccgagc gccgcatcca gtgccaattg cgtgtgctgt ggggcgagca cggcgtcgtg
     3841 caccggctct tcacgccgat cgccgactgg caggccaagg cgcgcggcac cgtgacaggg
     3901 cgggcaacgc cgagcggcca ttacatcacc gagcaggtgc ccgacctgct gctcgacgac
     3961 atgctggagt tcttccggtg atcgatgcgc cctttcatcg cgcgctgcgg ctcgggctgc
     4021 atgggtttcc gcacccggag gcggaggaag aggtcgcaca gatggtgcgt ggcatctgga
     4081 cgccgcacgc gccgtggatc gtctcgaacg aggcaccggt cgacgcgctg ctgctgtggc
     4141 gcggcacgcg taccgacgac ccggtggacg ccgcagtgct gcgcgtcaat ctgcatcgct
     4201 ccggctcatc gcggcgcgac gcggaccggc ggctcgaacc gctgttcgtg cggcggccgc
     4261 tgcgccagtc cgcgctgcgg gtggcgctgg atgcggcgtt cgcacgcatc cagcgcctgc
     4321 aaaagtagga tcagcttcgc gcgcgctgcg ccagcgtcgg cgcgagttcc ccgtgcgccc
     4381 gctgcagcag ttcacgcgtc gtgtcccagc cgatgcaggc gtcggtcacc gacaccccgt
     4441 acgcgagctg cgacaggtcg tccgggatcg gttggttgcc ggcgttcagg ctgctttcca
     4501 gcatcagccc gacaatcgag cgattgcctt ccttgatctg gtgcacgcaa tccatgaaca
     4561 ccagcggctg cagcgacggc tccttcatcg aattggcgtg cgagcagtcg acgacgatgt
     4621 tggcgg
//
LOCUS       DBNHBY010000175         4043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1565831, whole genome shotgun sequence.
ACCESSION   DBNHBY010000175 DBNHBY010000000
VERSION     DBNHBY010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1565831"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2011
                     /gene="metG"
                     /locus_tag="AC16HB_04300"
     CDS             <1..2011
                     /gene="metG"
                     /locus_tag="AC16HB_04300"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR5606044.1"
                     /translation="YVQTDIWVRFQRMRGHDCLFIWASDAHGTPIMLKARQEGITPEA
                     LIERVSAQQRRDFVDFDISFDNFHTTHSDENREVTYEIYRRLVAGGHIRREKIVQAYD
                     EQAKMFLPDRYVRGTCPKCGAADQYGDSCEVCGATYTPADLLNAVSVVSGTAPVQRES
                     EHLFFRLGDFEPMLREWTGSGRLQAAVTAKLGEWFGAGLRDWDISRDEPYFGFEIPGE
                     TGKYFYVWLDAPIGYLSSHLNYCRRTGTDFDRYWKPDSQAEVYHFIGKDIVYFHTLFW
                     PAVLVGAGYRPPTSVYAHGFLTVNGQKMSKSRGTFITARTWLDHLPAEYLRYYFASRL
                     GDGVEDLDLNLDDFAAKLNSDIVGKLVNIASRCAGFIARGSGGRLAAELAEPALQQEF
                     VAAGERIAALYEGRDLAGAMREIMALTDRANLYIDQRKPWLMAKDPARAAEVQGVCTQ
                     GLNLFRLLALYLKPVMPRLAEGAERFLALPAQQWSDSAAPLLGTTINAYEPLATRVDP
                     AAVKSLLAAAAEDLKPSGVPQAAAATATAAPAASSKTTAAAASAPAAATAAAAPISID
                     DFGRVDLRIARIEAAELVDGADKLLKLGVDLGDGGRRTIFAGIRSAYEPAQLVGRLTV
                     VVANLAPRKMRFGTSEGMVLAAGPGGAEIFLLSPDAGATPGMKVK"
     gene            2031..2612
                     /gene="rsxA"
                     /locus_tag="AC16HB_04305"
     CDS             2031..2612
                     /gene="rsxA"
                     /locus_tag="AC16HB_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HIR5606045.1"
                     /translation="MTELLLLALGASLVNNFVLSNFLGLCPFVGVSRRFEAAAGMALA
                     TLFVLTLASGLSFVLWRWVLQPLHLEYLRTLTFILLIAGVVQFTELMVRATSPLLHQL
                     LGIFLPLITTNCAVLGVALLNVDRQHGLLESLVFGASAGAGFGLALLLFAALRERLET
                     ADVPAAFRGAPIALVTAGLMALAFMGFAGLGAR"
     gene            2609..3151
                     /gene="rsxB"
                     /locus_tag="AC16HB_04310"
     CDS             2609..3151
                     /gene="rsxB"
                     /locus_tag="AC16HB_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HIR5606046.1"
                     /translation="MNAVLLACAVAGGLALLIGAALGYAARRLPPSTDRLVDQIDALL
                     PQTQCGQCQYPGCRPYAQAIARGEADIDRCPPGGDETARALAQLLDREPRPVDPQYGT
                     SKGPLVAWIDEERCIGCARCLPACPVDAIVGAARYTHTVIAAQCTGCELCLPPCPVDC
                     IELHPVARTPAAERRLGRAA"
     gene            3151..>4043
                     /locus_tag="AC16HB_04315"
     CDS             3151..>4043
                     /locus_tag="AC16HB_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HIR5606047.1"
                     /translation="MARTNPFPAGVVLEAHKDAALRSPIRALAAPAQCVLSLDQGSGS
                     ACEPVVAVGDTVRVGTAIARPVDEFAATLHAPIAGHVVAIETRDAATASGRDTCIVIA
                     SDGSDAVEPAPWDPADLARMDAEEIVRALAAAGVAGLGGAAFPTATKLSAARSRGATQ
                     LLLNGAECEPWICCDDALMREAAADVLYGATVLQRALGAESCVVAVESDKPVAIAALQ
                     AAASAAPEARVSIQVVPAVYPQGAERQLVLDVFGVEVPARGLPADVGLTCQNVATAVA
                     VANWARTGQPLIERVVTVTGSG"
BASE COUNT          589 a         1449 c         1387 g          618 t
ORIGIN      
        1 gtacgtgcag acggacatct gggtgcgctt ccagcggatg cgcggccacg actgcctgtt
       61 catctgggcc agcgacgcgc acggcacgcc gatcatgctc aaggcgcgcc aggaagggat
      121 cacgcccgag gcactgatcg agcgcgtcag cgcacagcag cgccgcgact tcgtcgactt
      181 cgacatctcg ttcgacaact tccacacgac gcactcggac gagaaccgcg aagtcaccta
      241 cgagatctac cgtcgcctgg tggccggcgg ccacatccgc cgcgagaaga tcgtccaggc
      301 ctacgacgag caggcgaaga tgttcctgcc cgaccgctac gtgcgcggca cctgccccaa
      361 gtgtggcgcg gcggaccagt acggcgacag ctgcgaggtc tgcggcgcca cgtacacgcc
      421 ggccgacctg ctcaatgccg tgtccgtcgt cagcggcacc gcgccggtgc agcgcgagtc
      481 cgagcacctg ttcttcaggc tcggcgactt cgagccgatg ctgcgcgaat ggaccggcag
      541 cggccgcctg caggctgcag tcacggccaa gctgggcgaa tggttcggcg caggcctgcg
      601 tgactgggac atctcgcgcg acgagccgta cttcggcttc gagatcccgg gcgagaccgg
      661 caagtatttc tacgtctggc tcgacgcacc gatcggctac ctgtcgagcc acctgaacta
      721 ctgccgccgc accggcacgg acttcgaccg ctactggaag cccgacagtc aagccgaggt
      781 ctaccacttc atcggcaagg acatcgtcta cttccacacg ctgttctggc ccgccgtgct
      841 ggtcggtgcc ggctatcgcc cgccgacgtc cgtctacgcg cacggcttcc tgacggtcaa
      901 cggccagaag atgtcgaagt cgcgcggcac gttcatcact gcgcggacgt ggctcgatca
      961 cctgccggcc gagtacctgc gctactactt cgcgtcgcgt ctcggcgacg gcgtcgagga
     1021 cctcgacctg aacctcgacg acttcgccgc gaagctcaac tccgacatcg tcggcaagct
     1081 cgtcaacatc gcgagccgct gcgcgggctt catcgcgcgg ggctcaggcg gccggctcgc
     1141 ggcggaactc gccgagcccg cgctgcagca ggaattcgtc gccgcgggcg agcgcatcgc
     1201 ggcgctgtac gaaggtcgcg accttgccgg cgccatgcgc gagatcatgg cgctgacgga
     1261 ccgggccaat ctgtacatcg accagcgcaa gccctggctc atggccaagg acccggcccg
     1321 cgccgcggag gtgcagggtg tctgcacgca gggcctgaac ctgttccggc tgctcgcgct
     1381 gtacctgaag cccgtcatgc cgcgactcgc ggagggcgcc gagaggttcc tcgcgctacc
     1441 ggcgcagcaa tggtcggaca gcgcggcgcc gctgctcggc acgacgatca acgcctacga
     1501 gcctctcgcg acgcgcgtcg atccggcagc cgtcaagtca ctgctggccg cggcggccga
     1561 ggacctcaag ccgtccggcg tgccgcaggc cgctgccgcg accgcaaccg ccgcacctgc
     1621 agcgtcatcg aagacgaccg cggccgctgc atcggcacca gccgctgcaa cggccgctgc
     1681 ggctcccatc tcgatcgacg acttcggccg cgtcgacctg cgcatcgcgc gcatcgaagc
     1741 cgcggaactg gtcgatggcg cggacaagct gctcaagctc ggcgtggacc tgggcgacgg
     1801 cggccggcgc acgatctttg ccggcatccg gtcggcctat gagccggcgc agctggtcgg
     1861 gcggctcacc gtggtagtcg ccaacctcgc gccgcgcaag atgcgcttcg ggacatccga
     1921 aggcatggtg ctcgccgcgg gcccgggcgg cgcggagatc ttcctgttgt cgccggacgc
     1981 gggcgccacg cccggcatga aggtgaaata agcgcggccg acggaggcac atgaccgagc
     2041 tgctgctgct cgcgctcggg gcgagcctcg tcaacaactt cgtcctctcg aacttcctcg
     2101 ggctgtgccc gttcgtcggc gtgagccggc gcttcgaggc ggcagcgggc atggcgctgg
     2161 cgacgctgtt cgtcctgacg ctggcctccg gactgagctt cgtgctgtgg cgctgggtgc
     2221 tgcagccgct gcacctcgaa tacctgcgca cgctcacctt catcctgctg atcgcaggcg
     2281 tggtgcagtt cacggagctc atggtgcgcg cgacgagccc tctgctgcac cagctgctgg
     2341 gcatcttcct gccgctcatc accaccaatt gcgcggtgct gggcgtcgcg ctgctcaacg
     2401 tcgaccgcca gcacggcctg ctcgagtcgc tggtgttcgg cgccagcgcc ggagcgggct
     2461 tcggccttgc gctgctgctc ttcgccgctc tgcgcgaacg gctcgaaacg gcggacgtgc
     2521 cggccgcctt ccgcggcgca ccgatcgcgc tggtgaccgc cggactcatg gcgctcgctt
     2581 tcatgggctt cgcggggctc ggcgcgcgat gaacgccgtc ctgctcgcgt gcgccgtggc
     2641 gggcggcctg gcgctgctca taggtgctgc gcttggctac gccgcgcgcc ggctgccgcc
     2701 gtccaccgat cgtctcgtcg accagatcga cgccctgctg ccgcagaccc aatgcggcca
     2761 gtgccagtat cccggctgcc ggccgtatgc gcaggccatt gcgcgcggcg aagccgacat
     2821 cgaccgctgc ccgccgggcg gcgacgagac cgcgcgggca ctggcgcagc tgctcgaccg
     2881 cgagccacga ccggtcgacc cgcaatacgg aaccagcaag ggtcccctgg tcgcctggat
     2941 agacgaggag cggtgcatcg gttgtgcgcg ctgcctgccg gcatgcccgg tcgatgcgat
     3001 cgtcggcgcg gcccgctaca cccacaccgt catcgcggca cagtgcaccg ggtgcgagct
     3061 gtgcctgccg ccctgccccg tcgactgcat cgaactgcac ccggtcgcgc gtacgccagc
     3121 ggcggagcgc cgcctcggca gggccgcctg atggcgcgaa ccaacccctt ccccgccggc
     3181 gtcgtgctcg aagcgcacaa ggacgcggcg ctgcgatcgc cgatccgggc gttggcggcg
     3241 cccgcgcaat gcgtgctgtc gctcgaccag gggtctggtt cggcttgcga accggtcgtt
     3301 gcggtgggcg acaccgttcg cgtcggcacc gccatcgccc ggccggtcga cgagttcgcc
     3361 gcgacgctgc acgcgccgat cgccgggcac gtcgtggcca tcgaaacgcg cgatgcagcc
     3421 acggcgagcg gacgcgacac atgcatcgtc atcgccagcg acgggagcga tgcggtcgag
     3481 ccggcgccgt gggatccggc tgatctcgcg cgcatggatg cggaagagat cgtccgtgcg
     3541 ctggccgccg ccggcgtggc cggtctcggc ggcgccgcgt tcccgacggc caccaaacta
     3601 tctgcggcac gctcgcgcgg tgcgacgcag ctgctgttga acggcgcgga gtgcgagccc
     3661 tggatctgct gcgacgacgc gttgatgcgc gaggccgcag ccgacgtgtt gtacggcgcc
     3721 acggtcctgc agcgcgcgct cggtgccgaa tcgtgcgtgg tggccgtgga gagcgacaag
     3781 ccggtagcga ttgcggcctt gcaggctgct gccagtgcgg ccccagaggc ccgcgtatcg
     3841 atacaggtcg tgccggcggt gtatccacag ggcgccgagc ggcaactcgt gctcgacgtg
     3901 ttcggcgtcg aagtgcccgc ccgcgggctg ccggccgacg tcggcttgac gtgccagaac
     3961 gtcgcgacgg cggtcgcggt cgcgaactgg gcgcggaccg gacaacccct catcgagcga
     4021 gttgtcaccg tcaccggcag tgg
//
LOCUS       DBNHBY010000176         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1430447, whole genome shotgun sequence.
ACCESSION   DBNHBY010000176 DBNHBY010000000
VERSION     DBNHBY010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1430447"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="AC16HB_04320"
     CDS             complement(<1..1232)
                     /locus_tag="AC16HB_04320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5606048.1"
                     /translation="MKVAEKPAAIEPDTLIGKRIPKLDAPEKASGKTRYIHDINLPGQ
                     LHARILRSARVHARILRIDTSAAKALPGVHVVLTAADVPDQRPIGVAKDHLPLKGDRV
                     RSVRDEIAAVAADSAEIAEEALKLIKVEYEDLPVLANAVDAIRSDAVLIHPEPHGADG
                     THLHLKPNTPIAFSGKKDNIAMRFDYSHGDVAKGEAESDVVVEDTFKLHYVTHCCMGV
                     SGVIVDFDASGNLLMYSNTQVPFLHRREFAEYLNMDPARIRIIQPPIGGGFGSKLDIY
                     PFEVICVFLARAAKRPVKMVFTREEEFLASPTRQPVLLTLRSGCKRDGTLTFRQVHTL
                     HDNGAYTSWGATTPFVMMQTFSSLYRVPHCDYHTTAVYTNNPYAGSFRGYGNLQATFA
                     VEQHMDMMADAIGMDPLE"
     gene            complement(1229..1714)
                     /locus_tag="AC16HB_04325"
     CDS             complement(1229..1714)
                     /locus_tag="AC16HB_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5606049.1"
                     /translation="MSLKHEIAFTLNGVQRKVTVEVGMSTLAMLRDVIGLTGTKYGCG
                     EGECGACTIVVDGATVNACLMFAVDCDGRRVTTIEGLTGDARADALRAAFVAKGAVQC
                     GFCTPGLMVQATHLLEQHPHPDAETVKRGIEGNLCRCTGYKKIVEAVMTAGRAQEGVA
                     G"
     gene            complement(1698..2564)
                     /locus_tag="AC16HB_04330"
     CDS             complement(1698..2564)
                     /locus_tag="AC16HB_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5606050.1"
                     /translation="MNAIEQYAAPTTLAQALEALRGGEVTILAGGTDLMPQSASGKVK
                     FKPTLMNIQRVPELKGIARDGDAIRIGALVTVTEMMRDPLVQEHFPVLVEVGDHFASD
                     QIRNAATIGGNIVNASPAGDTLVPLLVLGAEVELATKPNGTITTRRLPLAEFFVGPGR
                     TKRAPNELLTGVRIPVPPRGHVARFYKFGTRPALDISAISIGIAAVTQDGVLSNVRVA
                     FGAVAPTPVRAPRTEAALEGKRLNRATIDAAAQTAHDEVKPIDDVRASAWYRRELIRN
                     MTQRMLDDVAQA"
     gene            complement(2573..>2977)
                     /locus_tag="AC16HB_04335"
     CDS             complement(2573..>2977)
                     /locus_tag="AC16HB_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5606051.1"
                     /translation="VDAEGGIVRLVSEGIVQARVSAQAYQRQREIESGRFRKVGVNCY
                     QSEAEPERVVEFHPYKEDDARAQIAALNRVRAERDHAAVATALARVADDARSGVNLMP
                     AIVDAVKAYASVGEITNELVKVFGRYVEPVRF"
BASE COUNT          419 a         1026 c          998 g          534 t
ORIGIN      
        1 aactcgagcg ggtccatgcc gatcgcgtcg gccatcatgt ccatgtgctg ctcgaccgcg
       61 aacgtcgcct gcaggttgcc gtagccgcgg aacgatccgg catacgggtt gttcgtgtag
      121 acggcggtcg tgtggtagtc gcagtgcggc acgcggtaca gcgacgagaa cgtctgcatc
      181 atcacgaacg gcgtggtcgc gccccacgat gtgtacgcgc cgttgtcgtg cagcgtgtgc
      241 acctggcgga acgtgagcgt gccgtcgcgc ttgcagcccg aacgcaacgt cagcagcacc
      301 ggctggcgcg tcggcgaggc gaggaactct tcctcgcgcg tgaacaccat cttgaccggg
      361 cgttttgccg cgcgtgcgag gaacacgcag atcacttcga acggatagat gtcgagcttg
      421 gagccgaagc cgccgccgat cggcggctgg atgatgcgga tgcgcgccgg gtccatgttc
      481 aggtactcgg cgaactcgcg ccggtgcagg aacggcacct gcgtgttcga gtacatcaag
      541 aggttgccgc tggcgtcgaa gtcgacgatc acgccggaga cgcccatgca gcagtgcgtg
      601 acgtaatgca gcttgaacgt gtcctcgacc acgacgtcgg attccgcctc gcccttggcc
      661 acgtcgccgt ggctgtagtc gaagcgcatc gcgatgttgt ccttcttgcc gctgaacgcg
      721 atcggcgtgt tcggcttcag gtgcaggtgc gtgccgtccg cgccgtgcgg ctcggggtgg
      781 atcagcaccg cgtcggatct gatcgcgtcg accgcgttgg cgagcaccgg caggtcctcg
      841 tattcgacct tgatcagctt cagcgcctcc tcggcgatct cggccgagtc ggccgccacc
      901 gcggcgatct cgtcgcgcac gctgcgcacc cggtcgccct tcagcggcag gtggtccttc
      961 gcgacgccga tcgggcgctg atccggcacg tcggcggcgg tcagcaccac gtgcacgccc
     1021 ggcagagcct tcgcggccga cgtgtcgatg cgcagaatcc tggcgtgcac gcgcgccgag
     1081 cgcaggatcc tggcgtgcaa ctgacccggc aggttgatgt cgtggatgta gcgcgtcttg
     1141 ccgctcgctt tctcgggcgc gtcgagcttc gggatgcgct tgccgatcag cgtgtcgggc
     1201 tcgattgccg cgggcttctc tgcgaccttc atcctgcgac tccttcctgc gcgcgacccg
     1261 cggtcatcac cgcttcgacg atcttcttgt acccggtgca gcggcacagg ttgccctcga
     1321 tgccgcgctt gaccgtctcc gcgtcgggat gcgggtgctg ctcgagcaga tgcgtcgcct
     1381 gcaccatcag tcccggcgtg cagaagccgc actgcaccgc gcctttggcg acgaacgcgg
     1441 cccgcagcgc atcggcgcgc gcgtcgccgg tcagcccctc gatcgtggtc acgcggcggc
     1501 cgtcgcagtc gaccgcgaac atcaggcacg cattcaccgt cgcgccatcg accacgatcg
     1561 tgcacgcgcc gcattcgcct tccccgcagc cgtacttggt gccggtcagc ccgatcacgt
     1621 cgcgcagcat cgcgagcgtg ctcatgccga cttccaccgt caccttgcgc tgcacgccat
     1681 tcagcgtgaa cgcgatctca tgcttgagcg acatcgtcga gcatcctctg ggtcatgttg
     1741 cggatcagtt cgcgccgata ccaggcggag gcgcgcacgt cgtcgatcgg cttcacttcg
     1801 tcgtgcgccg tctgcgcggc ggcgtcaatc gttgcgcggt tcagccgctt gccttcgagc
     1861 gcggcctccg tgcgcggcgc gcgcaccggg gtcggcgcca cggcgccgaa ggcgacgcgg
     1921 acgttcgaca gcacgccgtc ctgcgtgacc gccgcgatgc cgatcgagat cgcggagatg
     1981 tccagcgccg gccgcgtgcc gaacttgtag aagcgcgcga cgtggccgcg cggcgggacc
     2041 ggaatgcgta cgccggtcag aagttcgttc ggcgcgcgct tcgtcctgcc cggcccgacg
     2101 aagaactcgg cgagcggcag gcggcgcgtc gtgatggtgc cgttgggctt ggtcgcgagt
     2161 tccacttcgg cgccgagcac cagcagcggc accagcgtgt cgccggcggg cgacgcgttg
     2221 acgatgttgc cgccgatcgt ggctgcgttg cggatctgat cgctggcgaa gtgatcgccg
     2281 acctcgacca gaaccgggaa atgctcttgc accagcggat cgcgcatcat ctcggtcacg
     2341 gtgacgagcg cgccgatgcg gatcgcgtca ccgtcacgcg cgatgccctt cagctccggc
     2401 acgcgctgga tgttcatcag cgtcggcttg aacttcacct tgccgctggc gctctgcggc
     2461 atcaggtcgg tgccgccggc gaggatcgtg acctcaccgc cgcgcagggc ttcgagcgcc
     2521 tgggcgaggg tggtcggggc ggcgtactgc tcgatcgcat tcatcttttg cctcagaaac
     2581 ggacgggctc gacgtagcgc ccgaacacct tgaccagctc gttcgtgatc tcgcccacgc
     2641 tggcgtaggc cttgaccgca tcgacgatgg ccggcatcag gttcacgccc gagcgcgcat
     2701 cgtcggccac gcgcgcgagt gccgttgcca ccgccgcgtg gtcacgctcg gcgcggaccc
     2761 gattcaacgc cgcgatctgc gcgcgcgcgt cgtcctcctt gtatgggtgg aactcgacga
     2821 cgcgttcggg ttccgcctcg ctctggtagc agttgacgcc gaccttgcgg aaccggcccg
     2881 actcgatctc gcgctgccgc tggtacgcct gcgccgacac gcgcgcctgc acgatgccct
     2941 cggacaccag ccgcacgatg ccgccttcgg cgtcgac
//
LOCUS       DBNHBY010000177         3472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1498578, whole genome shotgun sequence.
ACCESSION   DBNHBY010000177 DBNHBY010000000
VERSION     DBNHBY010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1498578"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /gene="htpG"
                     /locus_tag="AC16HB_04340"
     CDS             complement(<1..1450)
                     /gene="htpG"
                     /locus_tag="AC16HB_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR5606052.1"
                     /translation="MGAIKETLGFQAEVKQLLHLMVHSLYSNKEIFLRELISNASDAC
                     DKLRFESIAHPELLDGGAELAIRVAYDKAARTITVSDNGIGMSRQEAIEHLGTIAKSG
                     TREFFAKLTGDQQKDAQLIGQFGVGFYSSFIVADRVSVISRRAGTSEAVKWESDGSGE
                     FTVEPAERTARGTDVVLHLKEGEDELLSHWKLKSILTKYSDHIATPIRMQKEEWDKDK
                     GAYAVKDEEETVNQASALWARAKADITDEQYKEFYKHVSHDFGDPLAWTHNRVEGKQE
                     YIQLLYVPGRAPFDLWDRNSRHGVKLYVRRVFIMDDAEQLMPPYLRFVRGVIDSNDLP
                     LNVSREILQESRDVKAIREGSTKRVLGLLEDLAENRKDDYAKFWAEFGQCLKEGIGED
                     TTNRERIAKLLRFSTTTSDAQSVTFADYVGRMKDKQKALYYLTADSLDAAKSSPHLEI
                     FRKKGVEVILFTDRVDEWMLTFLRDFDGKELVS"
     gene            1581..2288
                     /locus_tag="AC16HB_04345"
     CDS             1581..2288
                     /locus_tag="AC16HB_04345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5606053.1"
                     /translation="MANELHQLFDPTSSTYTYVLVSGPNRAAVIIDPVAEQFERDLAF
                     VRGRELALRYVIETHTHADHITSAGELARITGAFAAVPSGCGIAPAPVQLGDGDALWF
                     GLTSLKAIHTPGHTAGSMCYLWRDEHCVFTGDTLLIGGCGRTDFQSGDAGALYDSITQ
                     RLFTLPDDMRVYPAHDYHGQTSTSIGAERTGNPRLAGRSRDDFIALMQALNLPKPKLI
                     DVAVPANRQLGQVPHAA"
     gene            complement(2292..>3472)
                     /locus_tag="AC16HB_04350"
     CDS             complement(2292..>3472)
                     /locus_tag="AC16HB_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR5606054.1"
                     /translation="RRDARSVHAAREPAPAVDAGAPDVVWLLRSMLRQAPASPQPGAG
                     LLPAQWLDSDLVSAAVRAVGRSMGASALSYGLPEGYLPLREQIVQRLALADIGADASQ
                     IVTTCGVTQGIDLVARHFVAAGDTVFVEDPAWFVMFARFAAFGARVIGVPRRADGPDI
                     KVLRNLLTRHQPKLFVVGSVLHNPTCTTISAANAHELLKVADAYGFTIVEDDIYGDLH
                     PGRGAAVRLASLDQLKRVVYVAGFSKTLAASLRVGYVACARELAEQLANLKLLTGLTT
                     PEATERIVARVLAEGGYRRHLDRVRGKLDAARDRTARALERIGAKLFAPPAAGMFLWA
                     DFGRDTNAIAAEASQQDILCAPGSLFSPTQLPSPWMRVSAAASLNPAAMRFLAQTLER
                     "
BASE COUNT          530 a         1194 c         1149 g          599 t
ORIGIN      
        1 ccgataccag ttccttgccg tcgaagtcgc gcagaaaggt cagcatccac tcgtcgacgc
       61 gatcggtgaa caggatcacc tcgacgccct tcttgcggaa tatctccagg tgcgggctgc
      121 tcttcgcggc gtcgagcgag tcggcggtca ggtagtacag cgccttctgc ttgtccttca
      181 tccggccgac atagtcggcg aacgtcaccg actgcgcgtc gctcgtcgtg gtcgagaaac
      241 gcagcagctt tgcaatccgc tcgcgattcg tcgtgtcctc accgatgccc tccttcaggc
      301 actggccgaa ctcggcccag aacttcgcgt aatcgtcctt gcggttctcc gcgaggtctt
      361 cgagcagtcc gaggacgcgc ttggtcgagc cctcgcggat cgccttcacg tcgcgcgatt
      421 cctgcaggat ttcgcgcgac acgttcaacg gcaggtcgtt ggagtcgatc acgccgcgca
      481 cgaagcgcag gtacggcggc atcaactgct cggcgtcgtc catgatgaac acgcgacgca
      541 cgtacagctt gacgccgtgg cgactgttgc gatcccacag gtcgaacggc gcgcgccccg
      601 ggacgtacag gagctggatg tattcctgct tgccctcgac gcggttgtgc gtccacgcca
      661 gcgggtcgcc gaagtcgtgg ctgacgtgct tgtagaactc cttgtactgc tcgtcggtga
      721 tgtcggcctt cgcgcgtgcc cacagcgcgc tggcctggtt gacggtttcc tcttcgtcct
      781 tgaccgcgta cgcgcccttg tccttgtccc actcctcctt ctgcatgcgg atcggggtgg
      841 cgatgtggtc ggagtacttg gtcaggatcg acttgagctt ccagtgcgac agcagctcgt
      901 cctcgccctc cttcaggtgc aggaccacat cggtgccgcg cgcggtgcgt tctgccggct
      961 cgaccgtgaa ctcgccgctg ccgtcgcttt cccacttgac cgcttcgctc gtgccggcgc
     1021 gccgcgagat caccgatacc cggtcggcga cgatgaagct cgaataaaag ccgacgccga
     1081 actggccgat cagctgcgcg tccttctgct ggtcgccggt gagtttcgcg aagaattcgc
     1141 gcgtgccgct cttggcgatc gtccccaggt gctcgatcgc ttcctggcgc gacatgccga
     1201 tgccgttgtc ggacaccgtg atcgtgcgcg cggccttgtc gtacgcgacc cggatcgcaa
     1261 gctcggcacc gccgtccagc agctccggat gcgcgatcga ctcgaagcgc agtttgtcgc
     1321 acgcgtccga cgcgttgctg atcagctcgc gcaggaagat ttccttgttc gaatacagcg
     1381 agtggaccat caggtgaagc agttgcttca cctcggcctg gaagcccagc gtttccttga
     1441 ttgcacccat gacgatgttc ccggcaggtc gtggttgaca gttggcgcgg catggccgcg
     1501 ttcgcgttga tggggtcgaa ttctgccgct tcaaggcccc gcccgcttgg cagaccggcg
     1561 accaagcgct ctaatcgacc atggccaacg agctgcacca gttgttcgac ccgacgtcgt
     1621 cgacctacac gtacgttctg gtcagcggcc cgaaccgcgc cgcggtgatc atcgacccgg
     1681 tagccgagca gttcgagcgc gacctggctt tcgtgcgcgg gcgggagctg gcgttgcgct
     1741 acgtgatcga gacgcatacg cacgccgacc acatcacatc ggcgggggag ctggcacgca
     1801 tcaccggcgc cttcgccgcg gtgccgagcg gctgcggcat cgcgccggcg ccggtgcagc
     1861 tcggcgacgg cgacgccttg tggttcggcc tgacgagcct gaaggcgatc cacaccccgg
     1921 ggcacaccgc cggcagcatg tgctacctct ggcgcgacga gcactgcgtg ttcaccggcg
     1981 acacgctgct gatcggcggc tgcggccgca ccgatttcca gtcgggcgac gccggcgcgc
     2041 tgtacgacag catcacccag cggctcttca ccctgcccga cgacatgcgc gtctaccctg
     2101 cgcacgacta ccatggccag acgagcacgt cgatcggcgc cgagcgcacg ggcaaccccc
     2161 ggttggccgg ccgttcgcgc gatgatttca tcgcgctgat gcaggcgctg aacctgccga
     2221 agccgaagct gatcgacgtc gcggtgccgg cgaaccggca gctcgggcag gtgccgcacg
     2281 ccgcgtaggc ttcagcgctc gagggtttgc gcaaggaaac gcatcgccgc gggattcagg
     2341 ctcgccgccg ccgacacgcg catccatggg ctcggcaatt gcgtggggga gaacaggctg
     2401 ccgggcgcgc acaggatgtc ctgctggctc gcctcggcgg cgatcgcatt ggtgtcgcgc
     2461 ccgaagtcgg cccacaggaa catcccggcc gcggggggcg cgaagagctt ggcgccgatc
     2521 cgctccagtg cgcgcgcggt gcggtcgcgc gcagcgtcga gcttgccgcg cacccggtcg
     2581 aggtggcgcc ggtagccgcc ctcggccagc acgcgtgcga cgatgcgctc ggtcgcctca
     2641 ggcgttgtca gcccggtcag cagtttcaga ttcgcgagtt gctccgcaag ttcgcgcgca
     2701 caagcgacat atccgacgcg caaactcgcc gccagcgtct tggagaatcc ggcgacgtag
     2761 acgacgcgct tgagctggtc gaggctcgcc agccgcaccg ccgcgccacg accggggtgc
     2821 aggtcgccat agatatcgtc ctcgacgatc gtgaagccgt acgcgtcggc caccttcagc
     2881 agttcgtgcg cattggcggc ggagatcgtc gtgcaggtcg ggttgtgcag caccgagccg
     2941 accacgaaga gtttcggctg gtgccgcgtc agcaggttgc gcagcacctt gatgtcgggt
     3001 ccgtcggcgc ggcgcggcac gccgatcacg cgcgcaccga acgcggcgaa gcgggcgaac
     3061 atcacgaacc acgccgggtc ctcgacgaac accgtgtcgc cggccgccac gaagtggcgc
     3121 gcgacgaggt cgatcccctg cgtcacgccg caggtggtga cgatctgcga ggcatcggcg
     3181 ccgatgtcgg cgagcgcgag ccgctgcacg atctgctcgc gcagcggcag gtagccctcg
     3241 ggcaggccgt acgagagcgc ggaggcaccc atgctgcgcc ccaccgcgcg caccgcggcg
     3301 ctgacgaggt cggagtcgag ccactgtgcc ggcagcagcc cggcgccggg ttgcggcgat
     3361 gccggtgcct gccgcagcat cgaccgcagc agccacacca cgtccggcgc accggcatcg
     3421 accgcggggg ccggctcgcg cgccgcgtgg acacttcgtg catcgcgccg cg
//
LOCUS       DBNHBY010000178         3241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1295578, whole genome shotgun sequence.
ACCESSION   DBNHBY010000178 DBNHBY010000000
VERSION     DBNHBY010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1295578"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..648)
                     /locus_tag="AC16HB_04355"
     CDS             complement(364..648)
                     /locus_tag="AC16HB_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606055.1"
                     /translation="MKFVPAVEVEGPGAELPPARRVLHALLLHAAERAAHLAARLEAA
                     EVRARAPAEVVAAVRPYAPAGALGVKLAEAVALLAVAGYFVAGLVGVFVA"
     gene            1296..1739
                     /locus_tag="AC16HB_04360"
     CDS             1296..1739
                     /locus_tag="AC16HB_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606056.1"
                     /translation="MATTDTPAFTWVGIDPAEWLRSWQSAWRLAPENLTQPILPGWTF
                     NINSNNSSSPQTEVDVVAKHSYGRQLGRIADALALLIVEQHGETPADARYAEFLQMKR
                     EIDQVKEAGAAARIERFKKDLELLKANSTPEYERLRDELRRVLKG"
     gene            2018..2335
                     /locus_tag="AC16HB_04365"
     CDS             2018..2335
                     /locus_tag="AC16HB_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606057.1"
                     /translation="MKTDELEKQLVRAKKGAAKLAVRAQEQIGMRTDELEKQLTRAKK
                     GAGKLAVRAQEQATAATKVVRRSMKEAVVALKRATNKAARRVMEATAPAKPMKATRRH
                     AAG"
     gene            2415..>3241
                     /locus_tag="AC16HB_04370"
     CDS             2415..>3241
                     /locus_tag="AC16HB_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606058.1"
                     /translation="MRSRAERRAARAVLQRLQQRLADYGNDARADKLSLLTALRDLHL
                     DRAADLQALQRIICFVCAFPDDARALRAARRLATGFAERVARLPRTERAALEDSGMAG
                     TTTRHVYSFTAARWLARRYPDTTEIDWKGYECPESLSAVLQPLLDPFEDEHTEFDAAG
                     VRAWIGRAKVGRAGSDLRWLVAQAPATRKDAQAFEQAYDAAEVPIEWRIGTSRAAIVH
                     NAFAQRPHFRADGMRRPADDPRAAISEPLASIALPPRREAVRLLDVWRTALWSRTRNV
                     "
BASE COUNT          581 a         1073 c         1124 g          463 t
ORIGIN      
        1 tcaccggcat cggtgccggg tccggcagag tgttcgtcga gcgcagcgtt cgctgtgctg
       61 tcttggtcca ggtgcggggt cggttcgagc agcgatgcgg cagcgccgcc gctgctcacg
      121 aggggcaggg tggtcatcga aatctccagc attgcccccg ccgtgtggcc gagggcgtct
      181 cgctgagtgc ggcgccagcg gctggaggtt caaactcatt tcgggtcgac gccgaagtca
      241 agccgattcg attcgatagt cggggcgaag cggcaaggtg cgttgcgatc gacgcgacac
      301 accctgccga ctccggcttc caacgcaact tcgcacatcg aaatgatcgg catcaccgtc
      361 tcgctacgcg acgaagacgc cgaccaggcc ggcgacgaag tacccggcca ccgcgagcag
      421 cgcgacggcc tccgccagct tcacgccgag cgcgcccgcg ggcgcgtacg gccggaccgc
      481 ggcgacaacc tctgccggcg cgcgcgcccg cacctcggcc gcttcgagcc gcgcggccag
      541 atgcgccgcg cgctcggcgg catgcagcag cagcgcatgc aacacgcgcc gggcgggggg
      601 cagttccgca cccgggcctt cgacttcgac tgcagggaca aacttcaaga ctcgcaacat
      661 tgtgatcacc cttgatttcg cttggcgccg cgcctcagtc gctgcgcgac gcgtgattga
      721 ttaaggtccc tcgtcgaggg cttcctgttc caggtgcagt ccgcgccgca tgcgctcgta
      781 tgtcgacggg acacgctcgc ttcgaaccag attcgcaaac gacatgacac ctcctatctg
      841 ccgcgcaagg cggcaagcaa gaaagaaaac gtgagcctga aaagcaaaag cccgggtgca
      901 tcgctgcaac ccgggccgct tgcggagcgg tgacgcgcta tgtgacgcgc gcatcccggg
      961 ttggtcgatg cagggcgctg gccgccgcgg cccacacgac ggcggcagcc atgcccaggc
     1021 agcgttcggc gttcattgcg aacatctggc tactcctcgt ctaggtgggt gggcccggct
     1081 gtgatggcgc gccagcgtcg aatcgattcg cttgcgttcg ccgtcacgga agggacgatg
     1141 tgcgaatgat agttggtcac cgcgcttctg caatagccga cgtgcgtgaa tattcgcgcg
     1201 cgcgcgatta cagatgcaat catcgcggcg ccgatagact gcgcggccgg cgcgaactgt
     1261 gcgtgcgccg cggatcagcg aggagcagac gacccatggc aaccaccgac acgccggcgt
     1321 tcacctgggt tggcatcgac cccgccgaat ggctgcgctc gtggcagtcc gcgtggcgct
     1381 tggcgccgga aaacctgacg cagccgatcc tgcccggctg gaccttcaat atcaacagca
     1441 acaactcgtc gtcgccgcag accgaagtcg acgtggtggc gaagcacagc tacggccggc
     1501 aactcggccg catcgccgat gcgctggcgc tgctgatcgt cgagcagcac ggcgagacgc
     1561 ccgccgatgc gcgctacgcc gagttcctgc agatgaagcg cgagatcgat caggtgaaag
     1621 aggcgggcgc cgcggcgcgg atcgagcgct tcaagaagga cctggaactg ctgaaggcca
     1681 acagcacgcc ggagtacgag cggctgcgcg acgagctgcg gcgcgtgctg aaaggctgag
     1741 cgcctagacg aatccgtcgg caagacaccg ttcctgaccc gcggcagtgc gcgcgcgccc
     1801 gtactgccta cagtgcaatc gggttggcgc gatgtccgcg ccctccgcac atgcgatgga
     1861 aggagtccat atggtcacgc gtcgcactac gttgagaagt cgcacgggta cgaagctgta
     1921 cgccgtacgc gacagcagcg gcaagttcaa ggacatccaa agctacaaac gcgcgcacgc
     1981 ggcagatctg cgccggaagt cagcggcgga aatcggcatg aagaccgatg aactcgagaa
     2041 gcagctcgta cgcgcgaaaa agggtgccgc gaaactggcc gtccgcgcgc aggagcaaat
     2101 cggcatgagg accgatgaac tcgagaagca gttgacacgc gcgaagaaag gcgccgggaa
     2161 actggctgtc cgagcgcagg aacaagctac cgccgccacg aaggtggtcc ggcgttcgat
     2221 gaaggaggcc gtggtcgcct tgaagcgcgc aacgaacaag gccgcgcgcc gggtgatgga
     2281 agccaccgcg cccgcgaagc ccatgaaagc cacgcggcgc cacgctgcgg gctgaaggtt
     2341 agacacaggg cacgctgcgg atggtctccg ccgctccccg cccgcgctaa agtgcgcggc
     2401 ggggaggacg ggcgatgaga tcacgcgcgg agcggcgggc ggcgcgggcc gtacttcagc
     2461 ggctgcagca gcgtctggcc gactacggca atgacgcgcg cgccgacaaa ctctcgctcc
     2521 tgacagcgtt gcgcgatctg cacttggatc gcgccgccga cttgcaggcg ctgcagcgga
     2581 tcatctgctt cgtttgcgcg ttcccggacg atgcgcgcgc gctgcgcgcc gcgcggcggc
     2641 tcgcgacggg atttgccgag cgcgtggcgc ggttgccgcg cactgaacgc gccgcgctcg
     2701 aagacagcgg catggccggc acgacgacgc gccacgtgta ttcgttcacc gccgcgcgat
     2761 ggctcgcgcg gcgctatccg gacacgaccg agatcgactg gaagggctac gagtgcccgg
     2821 agtcgctgtc ggccgtgctg cagccgctgc tggatccgtt cgaggacgag cacaccgagt
     2881 tcgacgccgc cggcgtgcgc gcgtggatcg ggcgcgccaa ggtcggccgc gccggctccg
     2941 atctgcgctg gctggtcgcg caggcgccag cgacccggaa ggacgcgcag gcgttcgagc
     3001 aggcctatga cgctgccgag gtgccgatcg aatggcgcat cgggacgagc cgcgccgcga
     3061 tcgtgcacaa cgcattcgcg cagcggccgc acttccgcgc cgacggcatg cgcagaccgg
     3121 cggacgatcc gcgggcagcg atttccgagc cgctggcgag catcgcgctg ccgccccggc
     3181 gcgaggccgt gcgcctgctc gacgtgtggc gcacggcgct gtggtcgcgc acgcgcaacg
     3241 t
//
LOCUS       DBNHBY010000179         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1024834, whole genome shotgun sequence.
ACCESSION   DBNHBY010000179 DBNHBY010000000
VERSION     DBNHBY010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1024834"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16HB_04375"
     CDS             complement(<1..184)
                     /locus_tag="AC16HB_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HIR5606059.1"
                     /translation="MTAVAMRKPDLVCPAGSLRALTVAIDAGADAVYMGLKDATNARN
                     FAGLNFDLASTRAGIRY"
     gene            complement(181..1416)
                     /locus_tag="AC16HB_04380"
     CDS             complement(181..1416)
                     /locus_tag="AC16HB_04380"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR5606060.1"
                     /translation="MIVTDLPRLRLAGRELLPIVQGGMGVGVSAHRLAGSVAECNAVG
                     TLSSVDLRRHHPDLMARTARLGEGAKEAVNVANLEALAREIRAARELAQGRGLLAVNV
                     MRAVSEYAPYIRRACENGIDAVVVGAGLPLDLPDLCADFPRVALVPILSDARGIALIL
                     KKWERKGRLPDAIVIEHPKLAGGHLGAARIEELADPRFDFENVLPEARALLRALGAEE
                     RVPLIPAGGINSPQRVRQLFSLGASAVQIGTAFAVTREGDAHPEFKRVLAEAKPEDIV
                     EFVSVAGLPARAVKTPWLERYMSLAGKLQAKVAERGRGKSRCTLAFDCLAQCGLRDGI
                     AKFGQFCIDNQLAAALRGDVAHGLFFRGAGKLPFGSQIRSVRELIAFLLDKPIAEVAA
                     VGQKVSDKVSALLAEPVAA"
     gene            1871..2122
                     /locus_tag="AC16HB_04385"
     CDS             1871..2122
                     /locus_tag="AC16HB_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606061.1"
                     /translation="MLMKVDRLDIGVGWLLAVTLLALVLSAASAPGAAAYAAAFFAVQ
                     RLQSESLAMGRARPGPVSAVVQTSAVSDPDALLRTSRRH"
     gene            complement(2177..2407)
                     /locus_tag="AC16HB_04390"
     CDS             complement(2177..2407)
                     /locus_tag="AC16HB_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5606062.1"
                     /translation="MFIGCSDSRIVPYALMDCGPGDLFMARNVGNLAPPWDMSAEFHG
                     TANSIAAATRPPGDTVTEIDASHWANFAVPDR"
BASE COUNT          424 a          918 c          896 g          427 t
ORIGIN      
        1 catagcggat gcccgcgcgc gtcgacgcaa ggtcgaagtt cagcccggcg aaattgcgcg
       61 cgttggtcgc gtccttgagc cccatgtaga ccgcgtcggc gccggcgtcg atcgcgaccg
      121 tgagcgcacg cagcgatccc gccgggcaga ccagatccgg cttgcgcatt gcgacggcgg
      181 tcatgcggcg accggctccg ccagcagcgc gctgaccttg tcgctgacct tctgaccgac
      241 ggcggcgact tccgcgatcg gcttgtcgag caggaacgcg atcagctcgc gcaccgagcg
      301 gatctgcgag ccgaacggca gcttgccggc gccgcggaag aacaggccgt gcgccacgtc
      361 gccgcgcaac gctgcggcga gctggttgtc gatgcagaat tggccgaact tggcgatgcc
      421 atcgcgcagc ccgcactgcg ccaggcagtc gaacgccagc gtgcagcgcg acttgccgcg
      481 gccgcgttcg gcgaccttgg cctgcagctt ccccgccagg ctcatgtaac gctccagcca
      541 gggcgtcttc accgcgcgcg ccggtagccc ggcgacgctg acgaattcaa cgatgtcctc
      601 cggcttcgcc tccgccagca cgcgcttgaa ctccggatgc gcgtcgccct cccgcgtgac
      661 cgcgaaggcc gtgccgatct gcaccgccga ggcgccgagc gagaacagct ggcgcacgcg
      721 ctgcggtgag ttgatgccgc cggcgggaat cagcggcacg cgctcctccg cgccgagcgc
      781 gcgcagcaac gcgcgcgcct cgggcagcac gttctcgaaa tcgaagcgcg gatcggcaag
      841 ctcctcgatt cgcgcggcgc cgaggtggcc gccggcgagc ttcggatgct caatcacgat
      901 cgcatcgggc agccggccct tgcgttccca cttcttcagg atcagcgcga tgccgcgcgc
      961 gtccgacagg atcggcacca gcgccacccg cgggaaatcc gcgcacaggt cgggcagatc
     1021 gagcggcagt ccggcgccga ccacgaccgc gtcaatgccg ttctcgcagg cgcgccggat
     1081 gtagggcgca tattcggaca ccgcgcgcat cacgttcacc gccagcagcc cgcgcccctg
     1141 agcgagttcg cgcgcggccc ggatctcgcg cgcgagcgct tcgagattcg cgacgttgac
     1201 cgcctccttc gcgccttcgc ccagacgcgc ggtgcgtgcc atcaagtccg gatgatggcg
     1261 ccgcaagtcg accgacgaca gcgtgccgac cgcgttgcac tcagccaccg agccggcgag
     1321 ccggtgcgca gaaacgccga cacccatgcc gccttggacg atcggtagca gttcgcgtcc
     1381 ggccagtcgc aatcgaggca gatcggtcac gatcatgcgc gcgccgtcag ttcggcctcg
     1441 accggaagac gggcgggcag cagcgggtcg gagaacaatt cggccatcgc atcgacaatc
     1501 ggatcggcaa atggacgcaa gtacgcacgc tgtaccgggc gtccagtcgg gcgagtgtga
     1561 ggcgcggggc cgggtgattc attgagcctt atcaagactg gccgtcccga ccctccatga
     1621 cgaaggggat cgatcggcgt gaacgcgtgg tgagccccac agaaaatcgg cgagtaagag
     1681 tcacgccttg ctcgtgccgt ggcgcccgac gaatggcgag gtgagtgtcg actcgattta
     1741 cagcctcgcg cggcccgtca tttgatcctt cgccaagggc ttgaagcgaa agccccgcgg
     1801 actcgttggc atgcgccttg cagcgatcag tgcgaacgcg ctgtgagccc gtatccggat
     1861 ctggcaggag gtgctcatga aggtcgacag actcgatatc ggcgtaggct ggctgctcgc
     1921 ggtcacgctg ctggcactgg tgctttcggc cgcgtcggca cccggcgcgg ccgcatatgc
     1981 ggcggctttc ttcgccgttc aacgcctcca gtccgaatcg ctcgccatgg gccgtgccag
     2041 gcccggtcca gtctctgccg ttgtccaaac gtccgcggtc tctgatccgg acgcactgct
     2101 gcgcaccagc cgccggcact gaacggtctt tcacgcggtt ggtctttgcc ccgcgccgtg
     2161 cgcggggttt ttttgctcag cgatcgggaa cggcgaagtt tgcccagtgc gatgcgtcga
     2221 tctcggtcac cgtgtcgccc ggcgggcgcg tcgcggcggc gatcgagttc gcggtgccat
     2281 ggaactccgc cgacatgtcc catggcggcg ccaggttgcc gacgttgcgc gccatgaaga
     2341 ggtcgcccgg accacagtcc atcagcgcat acggcacgat ccgtgaatcg gagcagccga
     2401 tgaacagcgt cttcggttgc tgtccttatt cgacgaggcg gcggtatgtt ttctcgtagc
     2461 gcgggaagaa attgcgctcg aaccgcgcca gccgcgccag caggtcgtcc attcagtgca
     2521 cgtgggcgtg cgactccgcg ccgagggcgt cgtgcagcag cgcgtgcgcg tattcgcgca
     2581 gagcgacgcg cagccggtgt tcggccaggt gcatcgccac gcggtcggcg accatctcgt
     2641 acggcagcag ttcgcccggc acgcg
//
LOCUS       DBNHBY010000180         4823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_348350, whole genome shotgun sequence.
ACCESSION   DBNHBY010000180 DBNHBY010000000
VERSION     DBNHBY010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_348350"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /gene="pgl"
                     /locus_tag="AC16HB_04395"
     CDS             <1..703
                     /gene="pgl"
                     /locus_tag="AC16HB_04395"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5606063.1"
                     /translation="TQIDVLADAAAVARAAADFIAAQARAAVTERGCFRIALSGGSTP
                     WLMLRELSSQDVPWANVHVFQVDERVAPAGDRARNLTCLRESLLAHASLPTANVHPMP
                     VEATDLVVAGARYAAELRECAGSPPILDLVHLGLGADGHTASLIPGDPVLDVVDRDVA
                     ITRPYQGRLRMTLTFPLLDRARCVLWIVTDAAKAAVLARLRAGDRSIPAARVRRDRAR
                     AFADRAAAAELGMRR"
     gene            747..3077
                     /locus_tag="AC16HB_04400"
     CDS             747..3077
                     /locus_tag="AC16HB_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5606064.1"
                     /translation="MQAFGAPGIDPTWSSSAKDIVGCALGPARLWFTSGFGILNEVYF
                     PRIDIPQIRDLGFIVADDSGFWVEVKRLKSHRAQVPHTGVPALEIVHPHARFELRLRL
                     APDPQRDVVLLQVTLQGDESLRPFALLAPHLGGSGRDNCAETTLYRGRRILWAEKPPF
                     GVALAAADAHQRDVWRATSAGYVGASDGWQDFARNGRLTWHFDRAGPGNVALIGELPR
                     AATLALGVSSSRESAATLAIAALCQPFDDVWDRHVGAWRDWQQRCSVPPDLPAPVREQ
                     LAISAMVLRVHQDKTFPGAMVASLSVPWGNAHDDVGGYHLVWPRDLVESAGGLLALGA
                     VAEARDILRYLIATQHDTGSWAQNQWLGGKAYWHGKQLDEAAFPVLLVAALAERDALD
                     GIQVAATVRSALAFIARNGPASEQDRWEEDAGINAFTLATCIAALVCGAAWLDEPARS
                     WALRLADDWNARIESWTLVHDTALARAHGVTGYYVRIAPTLSCGENGELGRILPIKNR
                     VRDPGIPADEQIATDFLQLVRLGLRDAHDPRVLDTLKLIDQQLKVDTPAGPAWHRYTG
                     DGYGEAEDGSPFRGTGCGRAWPLLTGERGHYELAAGRDPLPYLQAMAAMTGRCGLIPE
                     QVWDTDAIEERWLWPGRPTGSAMPLVWAHAEFVKLVASRALQRPFDRPEAVWRRYGGR
                     CPPSRHAIWTPRFPIARLRAGQTLCLLLPRPARVHYGFDGWQRVADVDTQDSGLALHA
                     APLPTPELVAGRRIDFTMFWTDTDTWEGHDYHVDID"
     gene            3052..4461
                     /gene="zwf"
                     /locus_tag="AC16HB_04405"
     CDS             3052..4461
                     /gene="zwf"
                     /locus_tag="AC16HB_04405"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5606065.1"
                     /translation="MTTTSTSTERAPELSDALVLYGATGDLAYKKIFPALQALVRRGR
                     FDMPVIGVALSGWNLDRLRERVRDSLRQHGVADDASAARLGELLRYVDGDYRAAATFQ
                     RVEQTLGAARRPLHYLAIPPSLFETAVEHIARLASTPTARVVVEKPFGRDLASAQALN
                     RSLRRVFAESAIFRIDHYLGKEAVQNLLFFRFANSFLEPLWNRNYLASVQITMAERFG
                     VEGRGRFYEEVGLVRDVVQNHLLQVVANLAMEPPVGTDSEALRDEKFKVFKSIRTLTG
                     ASLVRGQYAGYRNEPGVAPDSQVETFAAMQVHLDSWRWAGVPFFIRAGKQLPVTATEV
                     LVQLHRPPQDVFRDPLPPGKNYLRFQLGPGAVSIAIGALAKRPGTTMAGDEVELNVCR
                     TRDDEAGAYERLLGNAIHGDATLFARQDGVEQAWRIVDPILTSSKPAFPYQPGSWGPA
                     EAAAMTAEIGGWHAPADSA"
BASE COUNT          750 a         1648 c         1681 g          744 t
ORIGIN      
        1 tacgcagatc gacgtgctcg ccgacgccgc cgccgttgcg cgcgccgccg ccgacttcat
       61 cgccgcgcag gcgcgcgcgg cggttaccga acgcggatgc tttcggatcg cgctcagcgg
      121 cgggagcacg ccctggctga tgctgcgtga actctccagc caggatgtac cgtgggcgaa
      181 cgtgcatgtg ttccaggtcg acgagcgcgt cgcgcccgcc ggcgaccgcg cgcgcaatct
      241 cacctgcctg cgggaaagcc tgcttgcaca cgcgtcactg ccgaccgcca acgtacaccc
      301 gatgccggtc gaagcgaccg acctcgtcgt cgctggcgcg cgatatgcgg ccgaattgcg
      361 cgaatgcgcc ggttcgccgc cgatcctcga tctcgtgcat ctcggtcttg gagccgatgg
      421 gcacacggcg tcgctcatac ccggcgaccc ggtgctcgac gtcgtcgacc gcgacgtcgc
      481 gatcacgcgg ccctaccagg gcaggctgcg gatgacgctg acctttccac tgctcgatcg
      541 cgcgcgctgc gtgctgtgga tcgtgaccga tgcggcgaag gcggccgtgc tggcgcgcct
      601 gcgcgccggc gaccgctcga ttcccgccgc gcgcgtgcgc cgcgatcgtg cacgcgcgtt
      661 cgcggaccgg gccgcggcag cggaactcgg aatgcgccga tgaatcgacg aggtagcggc
      721 agaggcgcgc agaaggactc gtcacgatgc aggcattcgg agcgccggga atcgatccca
      781 cctggagcag cagtgcgaag gatatcgtcg gctgcgcgct cgggccggcg cggctgtggt
      841 tcaccagcgg cttcggcatt cttaacgagg tctactttcc gcgcatcgac attccccaga
      901 tccgcgatct gggattcatc gtcgcggacg acagcggctt ctgggtcgag gtcaagcgac
      961 tgaagtcgca tcgggcgcag gtgccgcaca ccggcgttcc ggcactggaa atcgttcatc
     1021 cgcacgcccg cttcgagctg cggttgcgac tcgcgcccga tccgcagcgc gacgtcgtgc
     1081 tgctgcaggt cacgctgcag ggcgacgagt cgctgcgacc gttcgcgctg cttgcgccgc
     1141 atctgggcgg cagcggccgc gacaattgcg ccgagacgac cctgtaccga ggtcgccgca
     1201 tcctgtgggc ggagaagccg ccattcgggg tggcgctcgc ggcagcggac gcgcaccaac
     1261 gcgatgtctg gcgcgcgacc agcgccggct acgtgggcgc cagcgacggc tggcaggact
     1321 tcgcgcgcaa cggccgcctc acctggcatt tcgaccgcgc agggccgggc aatgtcgcgc
     1381 tgattggcga gctgccccgc gcggcgacgc tcgcgctcgg agtcagctcg agccgcgagt
     1441 ccgccgccac gctggcgatc gcggcgctat gccagccgtt cgacgacgta tgggatcgtc
     1501 acgtcggcgc ttggcgcgac tggcagcaga ggtgcagcgt gccgcccgac ctgcctgcgc
     1561 cggtgcggga gcagctggcg atctcggcaa tggtcttgcg cgtacaccag gacaagacct
     1621 ttccgggcgc gatggtggcc agtttgagcg tcccctgggg caatgcgcac gacgacgtcg
     1681 gcggctatca cctcgtctgg ccgcgcgatc tcgtggagag tgcgggcggg ctgctcgcac
     1741 tgggtgcggt cgccgaggcg cgcgacatcc tgcgctatct gatcgccacg cagcacgaca
     1801 ctggcagctg ggcgcagaac cagtggcttg gcggcaaggc gtactggcac ggtaagcaac
     1861 tcgacgaagc ggcgttcccg gtgctgctcg tcgccgcgct cgccgagcgc gacgcactgg
     1921 acggtatcca agtcgcggcg acggtgcgca gcgcgttggc cttcatcgcc cgcaacggtc
     1981 cggcgtccga acaggaccgc tgggaggaag acgccggcat aaacgcgttc acgctggcga
     2041 cctgcatcgc ggcgcttgtc tgcggcgcag cgtggctgga cgagccggcg cgcagctggg
     2101 cgttgcgtct ggccgacgac tggaacgcac gcatcgagag ctggacgctg gtgcacgaca
     2161 ccgcactcgc gcgggcacac ggcgtgaccg gctattacgt ccgcatcgcg ccgacgctgt
     2221 cgtgcggtga aaacggtgag cttggccgca tcctgccgat caaaaaccgg gtacgcgacc
     2281 ccggcatccc ggccgatgaa caaattgcta ccgacttcct tcaactggtg cgcctggggc
     2341 tgcgcgatgc gcacgatccg cgcgtgctcg acaccctgaa gctgatcgat cagcagctga
     2401 aggtcgacac gccagccgga ccggcgtggc accgatatac cggcgatggc tacggcgagg
     2461 cggaggacgg cagtccgttc aggggcacgg ggtgcggccg cgcatggccg ttgctcaccg
     2521 gtgaacgagg ccactacgag ctggcagccg ggcgcgaccc gctgccgtac ctgcaggcga
     2581 tggcagcgat gaccggccga tgcggactga tcccggagca ggtgtgggac accgatgcga
     2641 tcgaagagcg ctggctttgg ccgggcaggc cgaccggttc ggcgatgccg ctcgtctggg
     2701 cccacgcaga gttcgtcaag ctcgtcgcca gccgcgcgct gcagcggccg ttcgaccgtc
     2761 ccgaggcggt gtggcggcgc tatggcgggc ggtgcccgcc ctcgaggcac gcgatctgga
     2821 cgccgcgctt tccgattgcg cggctgcggg ccgggcagac actgtgcctg ctgctgccgc
     2881 gcccggcacg cgtgcactac ggcttcgatg gctggcagcg cgtcgccgac gtggacacgc
     2941 aggacagcgg ccttgccctt catgccgcgc ccctaccgac cccggagctg gtggcgggca
     3001 gacgcatcga cttcaccatg ttctggaccg acaccgacac ctgggagggc catgactacc
     3061 acgtcgacat cgactgaacg cgcaccggag ctcagcgacg cgctggtgct gtacggcgcg
     3121 accggcgacc tcgcgtacaa gaagatcttt ccggcgctgc aggcgctcgt gcggcgtgga
     3181 cgcttcgaca tgccggtcat cggcgtcgca ttgagcggct ggaacctcga tcgtctgcgc
     3241 gagcgcgtgc gcgacagcct gcggcagcac ggcgtcgccg acgacgcctc ggccgcgcgg
     3301 ctcggcgaac tgctgcgcta tgtcgacggc gattaccgcg cggcggcgac atttcagcgc
     3361 gtcgagcaga cgctcggcgc ggcgcggcgg ccactgcatt acctagcgat tccgccaagc
     3421 ctgttcgaaa ccgcggtcga gcacatcgcc cgccttgcca gcacgccgac ggcgcgcgtc
     3481 gtggtcgaga agccgttcgg acgcgatctg gcgtcggcgc aggcactcaa tcgcagtctt
     3541 cgccgcgtgt ttgccgagtc ggcgatcttc cgcatcgatc attacctcgg taaggaagca
     3601 gtgcagaacc tgctgttctt ccgttttgcc aattcgtttc tcgagccgct gtggaaccgc
     3661 aactacttgg cgagcgtgca gatcacgatg gccgagcgat tcggcgtcga aggccgcggc
     3721 cgcttctacg aggaggtcgg cctggtgcgc gacgtggtgc agaaccatct gctgcaggtg
     3781 gtcgccaacc tggcgatgga accgccggtg ggcaccgaca gcgaagcgct gcgcgacgag
     3841 aagttcaagg tgttcaagtc gatccggacg ctgaccgggg cgagcctcgt gcgcggacag
     3901 tacgccggct accgcaacga gcccggggtg gcgccggact cgcaggtcga gacctttgcc
     3961 gcgatgcagg ttcatctcga ctcgtggcgc tgggccggcg tgccgttctt catccgtgcc
     4021 ggcaagcagc ttccggtcac cgcgaccgag gtgctcgtgc agttgcaccg gccgccgcag
     4081 gacgtgttcc gcgacccgct gccgccgggc aagaattacc tgcgtttcca gctcgggccg
     4141 ggcgccgtca gcatcgcaat cggcgcgctg gccaagcgac cggggacaac catggccggc
     4201 gacgaggtcg agctgaacgt atgccgcacg cgcgacgacg aggcgggcgc ctacgagcga
     4261 ctcctcggca atgccattca tggagatgcg acactgttcg cgcggcagga cggcgtcgaa
     4321 caggcgtggc gcatcgtcga cccgatcctg acgtcgtcga aaccggcgtt cccgtaccaa
     4381 ccggggtcct ggggtccggc cgaggcggcg gcaatgacag cggagatcgg cggctggcac
     4441 gcgccggccg attcggcgtg agccggacga agccgcgcgg tccgtgaaac ctcggtgcga
     4501 tgaaatccta tccttcggca tcacccgggt aacacgtctg cccacttgtt cttcgtcagg
     4561 cggagcatcc gccgacgccg gccgcccgga ttgcgcgaca ggacgcaggc ccggcgacgc
     4621 gacgatcgcg cgccgcacaa tgctgagcgg gggctccggc tgactactcg ctgggaccaa
     4681 cccgccatcc catcagaagc gctcgaccgg cgggaacggg tttatggctc gcggcggact
     4741 ggactcgaag ctgcctctcg ctcgccgccg tgccagccgt caacaccaac gaccgctatc
     4801 gcgaagccct actacgcacg tcg
//
LOCUS       DBNHBY010000181         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1498972, whole genome shotgun sequence.
ACCESSION   DBNHBY010000181 DBNHBY010000000
VERSION     DBNHBY010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1498972"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16HB_04410"
     CDS             <1..350
                     /locus_tag="AC16HB_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606066.1"
                     /translation="VQDGRMYVQSFPPHEEHPEAKAAAKGQRFNAKLQERMSQRAKQA
                     GLVLDWALVEKVVNGAQGIATPVSEAGLDLPMYAASARLVANAIPAGATWDGSDRPVP
                     IREAAASAAPEAR"
     gene            352..1524
                     /locus_tag="AC16HB_04415"
     CDS             352..1524
                     /locus_tag="AC16HB_04415"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5606067.1"
                     /translation="MQQPALQGLPAGWYREPARAALEAAAIFARSWQFVCHAADLPAP
                     GTAARFDCAGRSLFVLRTAAGGLRAFRNACVHRGARLIEGDAHTGLAFCVDQRVRCPY
                     HGWTYDDSGALLEAGTCAGSPQTPARTALRPAAVEVWRGLVFAAFDTPSASLAQGIGA
                     VGKEWPDLMPLRRLAEPLQQPVAADWKLACEHLLDVTHRNVAHPRSRPYAYGAADDAG
                     SNEHALGATAALAVSDDALWSARIYRRLVAALQEAPALEHWLYVWPNVLLQLTPDGLR
                     VRQLLPAGTGASVLRELRYGVPDSSRAMRRLRYAHQRLQRRARQEEVRLLARVQQGLA
                     SLESDDTLAVARDDAGLAWFAARCRSALVSIASDVVPVPAPAPRKRSPRRSEKTPA"
BASE COUNT          228 a          579 c          611 g          264 t
ORIGIN      
        1 gggtgcagga cggccgcatg tacgtgcagt cgttcccgcc gcacgaagag catccggaag
       61 ccaaggcggc ggccaagggc cagcgcttca acgccaagct gcaggagcgc atgtcgcagc
      121 gcgcgaagca ggcggggctg gtgctcgact gggcgctggt ggagaaggtc gtgaacggcg
      181 cgcagggcat tgccacgccg gtgtccgagg cgggactgga cctgccgatg tacgccgcga
      241 gtgcgcgact cgtcgccaac gccatccctg ccggcgccac ctgggacggc agcgatcggc
      301 cagtgccgat ccgcgaagcc gcggcgtctg ctgcgcccga ggcgcgctga tttgcagcag
      361 ccggcgttac aggggctgcc cgccggctgg tatcgcgaac cggcgcgtgc cgcgctcgag
      421 gccgcggcga tcttcgcgcg cagctggcaa ttcgtatgtc acgccgctga cctgccagca
      481 ccgggcaccg ctgcgcgctt cgattgcgcc gggcgatcgc tgttcgtcct gcggacggcc
      541 gcaggcggat tgcgcgcgtt tcgcaacgcc tgtgtgcatc gcggcgcgcg cctgatcgaa
      601 ggcgacgccc acacggggct cgcgttctgc gtcgaccagc gcgtgcgttg cccctaccac
      661 ggctggacct atgacgactc gggtgcgctg ctcgaagccg ggacgtgtgc cggttcgccg
      721 cagaccccgg cacgcaccgc cttgcgtcct gccgccgtcg aggtctggcg cggtctcgtg
      781 ttcgccgcgt tcgacacgcc ttccgcgtcg ctcgcgcagg ggataggcgc ggtcgggaag
      841 gagtggcccg acctgatgcc gctgcgccgc ctggccgagc cgctgcagca gccggtcgcg
      901 gctgactgga agctcgcctg cgagcacctg ctggacgtga ctcaccgcaa cgtcgcgcat
      961 ccgcgttcca ggccttacgc ctacggtgct gcggacgacg ccggctcgaa cgagcacgcg
     1021 ctcggtgcga cggctgccct cgccgtgagt gacgatgcgc tgtggtccgc acgtatttat
     1081 cggcggctgg tcgccgcgct gcaggaagcg cctgcgctgg aacactggtt gtacgtctgg
     1141 cccaacgtac tgctgcagct cacgccggac gggttgcggg tgcggcagtt gctgccggcc
     1201 ggcacgggcg ccagcgtgct gcgcgagctg cgctatgggg tgcctgactc gagccgggcc
     1261 atgcggcgcc tgcgttacgc gcaccagcga ctgcagcggc gcgcgcgaca ggaggaagtc
     1321 cggttgctgg cacgcgtcca gcagggcctc gcgtcgctgg agtccgacga cacgctggcc
     1381 gtggcgcgcg atgacgcagg gcttgcgtgg tttgccgccc gctgtcggag cgccctggtt
     1441 tcaatcgcat cggatgtcgt gcccgtgcca gctccggcgc cgcgcaagcg gagtccgcga
     1501 cggtcggaga agactccggc ctgagtcgcc ctcacccggc actgccgacc tctcccgcaa
     1561 tgcgggagag gtgagccaga acccattcca ttggggttgc gctgccggcc caaccctaca
     1621 agttgtgtgg taatttaccg gcgctttccc aggggcggca gttgcgcgag acggtacacg
     1681 gc
//
LOCUS       DBNHBY010000182         4992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_550901, whole genome shotgun sequence.
ACCESSION   DBNHBY010000182 DBNHBY010000000
VERSION     DBNHBY010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_550901"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..614)
                     /locus_tag="AC16HB_04420"
     CDS             complement(225..614)
                     /locus_tag="AC16HB_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5606068.1"
                     /translation="MNASKQVIDNRAAARSVLPGIEHETLACADNGVPTLSLWRQTIA
                     PGGATPPHRHDCDEVVLVLSGSGEVLLDGRVIPFGPDFTLAVPRNVPHQIVNTGDEPL
                     HTVAAFSKSPVGVAFPDGTPLELPWRS"
     gene            749..1639
                     /locus_tag="AC16HB_04425"
     CDS             749..1639
                     /locus_tag="AC16HB_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5606069.1"
                     /translation="MASLDRFLSFANAAQHGSFAKAARDLGLSPSAIAKNIARLEQQL
                     GLRLFHRTTRQVTLTQDGQRLFERCRRISDEVAALEDEAAGARAAVRGTLTLDAPFTY
                     GKQVVLPILQKLLSRHSELRLDLRLSDAYTDLVREGLDAVIRIGHLPDSRLVARMFDQ
                     QDLVVCASPAYLSRHGEPLTPDALSGHHCVVFRMPRTGRDRPWQFRSGRRAIELHPET
                     RVRVDDGEALVRAAATGMGLVQAPDYMAEEDLKRGRLVEVLKPYRPAPLPISVVYPSQ
                     RHLPPRVKALIQALTQRRTR"
     gene            1775..2716
                     /locus_tag="AC16HB_04430"
     CDS             1775..2716
                     /locus_tag="AC16HB_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606070.1"
                     /translation="MKRLLLAAVLALLNAAVAAQETRVAIGLSGWTGFAPLTLAKEAG
                     LFKKHGLDVTLRKIPQKDRHLAIASGDVQCAATTVETWIVWNANGVATTQIFQLDKSY
                     GADGMAVRNSIAKIADLKGKTVAASAPGTAPYFTLAWFLKKNGLSVKDVKIVNLEPQA
                     AANAFITGSDLDAAMTYEPYLSTVRAAPQAGKIIATTLDYPMIMDTFGCTPKFLAENP
                     QAAKALADSYFEAVEMIGKEPKKSFEIMGADVKQTAEQFEASQKYLRWQDRAANRKFF
                     AGEHAAFSKDAAELLLDVGIIKQIPDLSKLADTRFIQ"
     gene            2720..3493
                     /locus_tag="AC16HB_04435"
     CDS             2720..3493
                     /locus_tag="AC16HB_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606071.1"
                     /translation="MKPLRPVSTLSRAVLGAAFFVAFIAAWALATYGGFVPTTFLASP
                     GATLQAGWTLLVKFNFLHDVGMTVWRVIGGFAIAALIAVPLGLAMGAYKPIEAFFEPF
                     VSFARYLPASAFIPLLILWAGIGEEQKLAVIFIGAFFQLVLMIAVTVGATRRDLVEAA
                     YTLGADDRGILWRVMVPASLPTMAEQLRLVLGWAWTYVIVAELIGASSGIGHMITDSQ
                     ALLATDQIIFGIVVIGVIGLVSDFAFKRLNQRLFRWASL"
     gene            3490..4278
                     /locus_tag="AC16HB_04440"
     CDS             3490..4278
                     /locus_tag="AC16HB_04440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606072.1"
                     /translation="MSFLSVRAVEKTFAAAHRAGTTVALLPIDLEVAENDFVTVLGPS
                     GCGKSTLLRIVAGLESPTRGEVALEGRAIEGPGADRGMVFQSYTLFPWLTVRQNICFG
                     LREKGVPPAQQREISDRYVAEVGLRGFEDHYPKQLSGGMQQRTALARALANDPKILLL
                     DEPFGALDHQTRALMQELLLAIWEHHRKTVLFVTHDVDEAIFMANRCCVFSARPGRIK
                     CEVAVDLPTPRHYRMKTTPRFAEFKARLTEEIRVESIRTLEVTA"
     gene            4275..>4992
                     /locus_tag="AC16HB_04445"
     CDS             4275..>4992
                     /locus_tag="AC16HB_04445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606073.1"
                     /translation="MNERFDAIVIGGGVIGCAIAYHLARERARVLLLERGQIGQGTTA
                     QSSCILRTHYSVPENVALAQAAWRVYADFAAYLDDPDADCGLVECGYLIAAPAGDKAD
                     ALRDSLAAQRARGIDAREIDAATATRLLPIARFDDATLIGYEPRAGFADAWLVTSCFA
                     RNARRLGAAVREGVAATGLVCAGRRVIGAQTARGSIHAGVVISAQNIWSRELAQWCGI
                     VLPLTIERHTVITLDCPGTPY"
BASE COUNT          804 a         1691 c         1676 g          821 t
ORIGIN      
        1 tgcgcgcaac ggatgacgtt ccgcgagagc cagtgctgcg ggtggccggc atccgaatca
       61 gtgcctgatg ccgagcgccg ctgtgtccgc ggagcggccg atctcgatcc cgccaccgca
      121 gcgcagcgcc cttcggcacg cccggtactc gcgtgcggga acgacacgag cagcaccacg
      181 gctcggcgcc ggcggcgggg cgtatccccg caactgcacc gcgctcagct gcgccagggc
      241 agttcgagcg gcgtgccgtc gggaaacgcc acgccgaccg gcgacttcga aaacgccgcc
      301 accgtatgga gcggttcgtc gccggtgttg acgatctggt gcggcacgtt gcgcggcacc
      361 gcgagcgtga agtccggtcc gaacggaatc acgcgaccgt cgagaaggac ctcgccgctt
      421 cccgacagga ccaacacaac ctcgtcacaa tcgtgacggt gcggcggcgt cgcgccgccc
      481 ggcgcgatcg tctgccgcca caacgaaagc gtggggacgc cattgtccgc gcacgccagg
      541 gtctcgtgct cgatacccgg cagcacggag cgcgcggcgg cgcgattgtc gatcacctgc
      601 ttcgatgcat tcatgagctt gctccttgtc gatgtcggcg cggtgcgcag ccggtgacgc
      661 gcagtgtccg ccccttcgac cgcagcgtga acagccctca gagtcgcaac attgatgaca
      721 caatgtcggc aataactccg ggagtgtgat ggcgagcctc gaccgctttc tgtccttcgc
      781 gaatgccgca cagcacggca gcttcgcgaa agccgcgcgc gacctcggct tgtcgccgtc
      841 ggcgatcgcc aagaacattg cgcgactgga gcagcagctc ggactgcgcc tgttccatcg
      901 cacgacgcgc caggtcaccc tgacgcaaga cggacaaagg ctcttcgaac gctgccggcg
      961 catcagcgac gaagtggcgg cgctggaaga cgaggcagcc ggcgcgcgcg cggccgtgcg
     1021 cggcacgctg accctggacg cacccttcac ctacggcaag caggtcgtgc tgccgatcct
     1081 gcagaagctg ctgtcgcggc actcggagct gcgactcgac ctgcgactgt cggacgccta
     1141 caccgacctc gtccgcgaag ggctcgatgc cgtgatccgg atcggccacc tgcctgactc
     1201 gcgcctggtc gcgcgcatgt tcgatcagca ggatctcgtc gtgtgcgcga gcccggccta
     1261 cctgtcccgt catggcgaac cgcttacgcc cgacgcgctc tccgggcacc actgcgtggt
     1321 gttccggatg ccgcgcaccg gacgcgaccg gccctggcag ttccgcagcg gtcggcgtgc
     1381 gatcgaactg catcctgaga cgcgcgtgcg cgtcgatgat ggcgaagcgc tggtgcgcgc
     1441 ggcggccacc ggcatggggc tggtgcaggc gcccgattac atggccgaag aggacctcaa
     1501 gcgcgggcgc ctggtcgaag tgctgaagcc ctaccgcccg gcgccgctgc cgatctcggt
     1561 cgtgtatccg agccagcgcc atctgccgcc gcgggtaaag gccctgatcc aagcgctgac
     1621 tcagcgccgc acgcgctgac gatcaatgca aatcagcacc cctgcggcgg gagcatcgtg
     1681 cgcccctccg caactcgggc cagtcgccgc gcggtgcttg cgggcgccga acgcgcgccg
     1741 ataattcgca gtccccttga gaggagcagg accaatgaaa cgcttgttgc ttgctgctgt
     1801 gctcgcgctg ctcaacgcgg ccgtcgctgc ccaggaaacc cgggtcgcga tcgggctgtc
     1861 gggctggacc ggctttgcgc cgctcacgct cgccaaggaa gcgggcctct tcaagaagca
     1921 tgggctcgat gtgacgttgc gcaagatccc gcagaaggac cgccatctgg cgatcgcctc
     1981 tggcgacgtc cagtgcgcgg cgaccacggt cgagacctgg atcgtgtgga acgccaatgg
     2041 cgtggcgacc acgcagatct ttcagctcga caagagctac ggcgccgacg gcatggcggt
     2101 gcgcaactcg atcgcgaaga tcgcggatct caagggcaag accgtagcgg caagcgcacc
     2161 cggcaccgca ccgtacttca cgctggcgtg gttcctgaag aagaacgggc tgtcggtgaa
     2221 ggacgtgaag atcgtcaacc tcgagccgca ggcggccgcc aacgcgttta tcaccggcag
     2281 cgatcttgac gccgcgatga cctacgagcc gtacctgtcc acggtgcgcg cggccccgca
     2341 ggccgggaag atcatcgcca ccacgctcga ctatccgatg atcatggata cgttcggctg
     2401 cacgccgaag ttcctcgccg agaatccgca ggccgccaag gcgctcgccg acagctattt
     2461 cgaagcagtc gagatgatcg gcaaggagcc gaagaagtcg ttcgagatca tgggcgccga
     2521 cgtcaagcag actgcggaac aattcgaggc ctcgcagaag tacctgcgct ggcaggatcg
     2581 cgcggcgaac cggaagttct tcgcgggcga gcatgcggcg ttctccaagg acgcggcgga
     2641 actgctgctg gatgtcggaa tcatcaagca gattcccgac ctgtcgaagc tcgccgacac
     2701 gcgcttcatt cagtagccgt tgaagccgct ccggccggtt tcgacactgt cgcgcgccgt
     2761 gctcggcgcg gcgttcttcg ttgccttcat cgccgcgtgg gcattggcga cctacggcgg
     2821 cttcgtgccg acgacgtttc ttgccagccc gggcgcaacg ctgcaagccg gctggaccct
     2881 gctcgtgaag ttcaatttcc tgcacgatgt cggcatgacc gtctggcgcg tcatcggcgg
     2941 cttcgcgatc gcggcgctga tcgcggtgcc gctcgggctg gcgatgggcg cgtacaagcc
     3001 gatcgaggcg ttcttcgagc cgttcgtgtc gttcgcgcgc tatctgccgg cctcggcttt
     3061 catcccgctg ctgatcctgt gggcgggcat cggcgaggag cagaagctcg cggtgatctt
     3121 catcggcgcc ttcttccagc tcgtgctgat gatcgccgtc accgtcggcg ccacgcgcag
     3181 ggatctggtc gaggccgcgt acacgctcgg tgccgacgac cgcggcatcc tgtggcgcgt
     3241 gatggtgccg gcgtcgctgc ccacgatggc cgagcaactg cggctggtgc tcggctgggc
     3301 gtggacctac gtgatcgtcg ccgaactgat cggcgcctcg agcggcatcg ggcacatgat
     3361 caccgattcg caggcgctgc tggcgaccga tcagatcatc ttcggcattg tcgtgatcgg
     3421 cgtgatcggg ctggtgtcgg acttcgcgtt caagcggctg aaccagcggc tgttccggtg
     3481 ggcaagcttg tgagctttct ttcggtccgc gccgtcgaga agaccttcgc cgcggcgcac
     3541 cgcgcaggca cgaccgttgc cctgctgccg atcgacctgg aggtcgccga aaacgacttt
     3601 gtcacggtcc tcggtccgtc gggctgcggc aaatcgacgc tgctgcggat cgtcgccggc
     3661 ctggagtcgc cgacccgcgg cgaagtggcg ctcgagggcc gcgctattga aggccccggc
     3721 gccgaccgcg gcatggtgtt ccagagctac acgctgtttc cctggctcac cgtccggcag
     3781 aacatctgct tcggcctgcg cgagaagggg gtgccgcccg cgcagcagcg tgagatcagc
     3841 gaccgctacg tcgccgaggt cggcctgcgt gggttcgagg accactatcc gaagcagctt
     3901 tccggcggca tgcagcagcg caccgcgctg gcgcgggcac tcgccaacga tccgaagatc
     3961 ctgttgctcg acgagccgtt cggcgcgctc gaccaccaga cgcgcgcgtt gatgcaggaa
     4021 ctgctgctcg cgatctggga acatcatcgc aagaccgtgc ttttcgtcac gcacgacgtc
     4081 gacgaggcga tcttcatggc caaccgctgc tgcgtgttct ctgcacgccc cggccggatc
     4141 aagtgcgagg tcgcggtcga tctgccgacg ccgcgccact accggatgaa gaccacgccg
     4201 cgctttgccg aattcaaggc gcgcctgacc gaagagatcc gtgtcgagtc gatcaggacg
     4261 ctcgaggtca cggcatgaac gaacggttcg atgcaatcgt gatcggtggt ggcgtcatcg
     4321 gttgcgcgat cgcttaccac ctcgcgcgcg agcgtgcgcg cgtgctgctg ctcgagcgcg
     4381 ggcagatcgg gcagggcacg accgcgcaat cgtcgtgcat cctgcgcacg cactacagcg
     4441 tgccggagaa cgtcgctctg gcgcaggcgg cctggcgggt ctacgccgat ttcgccgcat
     4501 atctcgacga tccggatgcg gactgcggac tcgtcgagtg cggctacctg atcgccgcac
     4561 ccgcgggcga caaggccgac gcgctgcgcg actcgctcgc ggcgcagcgt gcgcgcggga
     4621 tcgacgcgcg ggagatcgat gccgcgaccg cgacccgcct gctgccgatc gcgcgcttcg
     4681 acgacgcgac gctgatcggc tacgagcccc gggccggctt cgccgacgcg tggctcgtca
     4741 cgagctgctt cgcgcgcaac gcacgccgac tcggtgctgc cgtgcgcgag ggcgtggccg
     4801 cgaccgggct cgtttgcgcg ggccggcgcg tgatcggtgc acagaccgcg cgcgggtcaa
     4861 tccacgccgg cgtcgtgatc agcgcgcaga acatctggag ccgtgagctc gcgcaatggt
     4921 gcggcatcgt gctgccgctg acgatcgagc gccatacggt gatcacgctc gattgccccg
     4981 gcacgccgta ca
//
LOCUS       DBNHBY010000183         5376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_754856, whole genome shotgun sequence.
ACCESSION   DBNHBY010000183 DBNHBY010000000
VERSION     DBNHBY010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_754856"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..689)
                     /locus_tag="AC16HB_04450"
                     /pseudo
     CDS             complement(1..689)
                     /locus_tag="AC16HB_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877964.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
     gene            complement(724..948)
                     /locus_tag="AC16HB_04455"
     CDS             complement(724..948)
                     /locus_tag="AC16HB_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HIR5606074.1"
                     /translation="MDLANLIRMANRIGDFFEAMPDADEARAGIAQHLQRFWEPRMRR
                     EIVAHVDAGGQTELKPLVLEAIRRHRALLV"
     gene            complement(952..1794)
                     /gene="fdhD"
                     /locus_tag="AC16HB_04460"
     CDS             complement(952..1794)
                     /gene="fdhD"
                     /locus_tag="AC16HB_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5606075.1"
                     /translation="MPSRTERRAPAAHAPTNEPVAEPLAVLRWGDGAVRTPLEVAREV
                     PVAFEYNGISHAVMLATPADLEDFALGFTLSEGIASRAEFYGVEAHTAANGITLAIDI
                     AAAAFMRLKSRRRTLAGRTGCGICGTESLDQVLRPLPDLSALEVRVSAAALARTYASL
                     HEHQPLQHATGAAHTAVWCDGGGAIQLAREDVGRHNALDKLIGALIAARVDTAAGYAL
                     VTSRASVEMVQKAASAGIAVLAAVSAPTALGVRIATDCGQTLIGFARGAEFCVYSHPE
                     RIHA"
     gene            complement(1778..4639)
                     /gene="fdhF"
                     /locus_tag="AC16HB_04465"
     CDS             complement(1778..4639)
                     /gene="fdhF"
                     /locus_tag="AC16HB_04465"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR5606076.1"
                     /translation="MQATALRRTRDDIDYGTPARTGSPVTLAIDGRAVTVPAGTSVLR
                     AAIEAGIEIPLLCATDSLEAWGSCRLCLVEIEGRKGTPASCTTPVEAGMRVRTQSDKL
                     SRLRRGVMELYLSDHPRDTDGRPIDRETQFGRLADDLGVRDVRYSSGAHHGAEPVDAS
                     NPYFAFDPALCIVCNRCVRACEETQGTYALTVDGRGFGSRISAGGLPFMQSECVSCGA
                     CVAACPTGSLREKSVLGLGAPDRTAITTCAYCGVGCSFKAELKDDAVVRMVPDRHGAA
                     NEGHACVKGRFAWGYATHPDRVTKPMIRDRFTDPWREVSWDEAISHAARRLREIQAQY
                     GRDSIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAG
                     TQTFKSVEHADVIVVIGANPTDAHPVFASRLKRRLRQGAQLIVIDPRQIELVSGAHVK
                     ADYHLQLKPGTNVAMLDALAHVIVTEGLVDESFVAARCEPTAFAQWREFIARPENSPE
                     RIEAHTGVPAALVRGAARLYATGGNAAIYYGLGVSEHAQGSTAVMAIANLAMATGNLG
                     REGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDTSVRGAFEAAWGVAILPEPGLR
                     IPNMFEAALTGSFKGLYCQGEDLVQSDPDTQHVAAALSALECLVVQDIFLNETARYAH
                     VFLPGSSFLEKDGTFTNAERRISRVRKVIEPLAGLADWEVTIGLAKALGCAMQYAHPS
                     EIMAEIARLTPTFAGVTYEKLDALGSIQWPCNDTAPAGTPIMHVDEFVRGKGRFIITR
                     YVPSDEKVTRRHPLLLTTGRVLSQYNVGAQTRRTDNVHWHHEDLLEIHPHDASERGIR
                     DGDWVGIESRAGQTVLRATVTERVAPGVVYTTFHFPESGANVITTDSSDWATNCPEYK
                     VTAVQVLRVTQPSEWQRRYQRFARAQEELYRERDRAVAN"
     gene            complement(4620..>5376)
                     /locus_tag="AC16HB_04470"
     CDS             complement(4620..>5376)
                     /locus_tag="AC16HB_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5606077.1"
                     /translation="VCGEETAMLESLEGRRGVVRAKPPLPAIAGLFGRPNVINNVVTL
                     ATVPIILARGAAFYRDYGVGRSRGTLTVQLAGNIRRGGLIEAAFGITLRELLTTWGGG
                     TASGRPIRAVQVGGPLGAYIPEHHWDIPLDYERFAEIGAVLGHGSVVVHDDTADLAQL
                     ARFAMEFCAFESCGKCTPCRVGSVRGVELIDDIIAGRGRPQRIALLRELCETMVDGSL
                     CAMGGMTPYPVLSALNHYPQDFGEGVADAGHRT"
BASE COUNT          788 a         1859 c         1855 g          874 t
ORIGIN      
        1 tcgcgccggt aaccgaccac gatccggccg agccgctcct tcaggtccgc cagcgacgtg
       61 gcgaggcaga agatcgccat cacctcgctt gccaccacga tgtcgaaggc atcctcgcgc
      121 ggaaacccgt tggcggggcc gccgagcccg atcgtgatcg agcgcagcgc gcggtcgttc
      181 atgtcgacca cacggcgcca cgtgacgcgc cggaaatcga agcgcagctc gttgttgtgg
      241 tggatgtggt tgtcgatcat cgccgccagc aggttgttgg cgagcgcaat ggcgttgaag
      301 tcgccggtga agtgcaggtt gatgtcctcc atcggcacca cctgcgcgta gccgccgccg
      361 gcggcgccgc ccttcatgcc gaacaccggg ccgagcgagg gttcgcgcag cgcgatgatc
      421 gcgcgcaggc cgatgcgatt tagcgcgtct gcgaggccca ccgtggtcgt ggtcttgcct
      481 tcgccgggcg gcgtcgggct catcgccgtg acgaggatca gcttgccgtc ggcgcgcgcg
      541 tcgatcgacg ccaggtagtc gagcgagatc ttggcctttt agcggccgta gggctcgatc
      601 gcgtcttccg ggatgtgcag ccggtcgcgt gccagggcaa cgatcggcag caggcgggcc
      661 gcctgcgcga tctcgatgtc ggtcttcatc gtctcccttg ttcttcttgt gccggtcgat
      721 gcgtcagacg agcagcgcgc gatgcctgcg gatcgcctcg agcacgagcg gcttcagttc
      781 ggtctgccct ccggcatcga cgtgcgcgac gatctcgcgc cgcatgcgcg gctcccagaa
      841 acgctgcaga tgctgcgcga tgccggcgcg cgcttcgtcg gcatccggca tcgcctcgaa
      901 gaaatcgccg atccggttcg ccatgcggat caggttcgcg agatccatct ttcaggcgtg
      961 aatccgttcc ggatggctgt agacgcagaa ttcggcgccg cgcgcgaagc cgatcagcgt
     1021 ctgcccgcag tcggtcgcga tgcgcacgcc gagcgcggtc ggcgccgata ccgccgcgag
     1081 gaccgcgatg ccggcagagg cggccttctg caccatctcc acgctggcgc ggctggtgac
     1141 cagcgcgtag ccggccgcgg tatcgacgcg cgcggcgatc agcgcgccga tcagcttgtc
     1201 gagcgcgttg tggcggccga cgtcctcgcg cgcgagctgg atcgcgccac cgccgtcgca
     1261 ccagacggct gtgtgcgcgg cgccggtcgc gtgctgcagc ggctgatgtt cgtgcagcga
     1321 cgcataggtg cgcgccagcg cggcggcgct cacgcgcacc tcgagcgccg acaggtcggg
     1381 cagcgggcgc agcacctggt cgaggctttc ggtgccgcag atgccgcagc cggtgcgccc
     1441 cgcgagcgtg cgccggcgcg atttcaggcg catgaaggcg gccgcggcaa tgtcgatggc
     1501 aagcgtgatg ccgttggccg cggtgtgcgc ttcgacgccg tagaactcgg cgcgcgaggc
     1561 gatgccttcg ctcagcgtga agccgagcgc gaagtcctcc aagtctgccg gcgtggcgag
     1621 catcactgcg tgcgaaatgc cgttgtattc gaacgcgacc ggcacctcgc gcgcgacttc
     1681 gagcggcgtg cgcaccgcgc cgtcgcccca gcgcaggacg gcgagcggct ccgcgaccgg
     1741 ctcgttggtc ggcgcgtgcg cagccggagc gcggcgttca gttcgcgacg gcacgatcgc
     1801 gctcgcggta aagctcttcc tgggcgcgcg cgaagcgctg gtagcgccgc tgccactccg
     1861 acggctgcgt cacgcgcagc acctgcaccg cggtgacctt gtattcaggg caattggtgg
     1921 cccagtccga actgtcggtc gtgatcacgt tggcgccgct ctccgggaag tggaaggtcg
     1981 tgtagaccac gcccggcgcc acgcgctcgg tcacggtcgc gcgcagcacc gtctggccgg
     2041 cgcggctttc gatgccgacc cagtcgccat cgcggatgcc gcgctcgctg gcatcgtgcg
     2101 gatggatctc gagaaggtcc tcgtggtgcc agtgcacgtt gtcggtgcgg cgcgtctgcg
     2161 caccgacgtt gtactgcgac agcacacggc cggtcgtcag cagcagcgga tggcggcgcg
     2221 tgaccttctc gtcgctcggc acgtagcggg tgatgatgaa gcggcccttg ccgcgcacga
     2281 attcgtcgac gtgcatgatc ggcgtgccgg cgggcgccgt gtcgttgcac ggccactgga
     2341 tgctgccgag cgcatcgagc ttctcgtagg tcacgcccgc aaaggtcggc gtcaggcgcg
     2401 cgatctcggc catgatctcg gaagggtgcg cgtactgcat tgcgcagcca agtgccttgg
     2461 cgagcccgat cgtcacttcc cagtccgcca gccctgccag tggttcgatc accttgcgca
     2521 cgcgcgagat gcgccgttcg gcattggtga aggtgccgtc cttctcgagg aacgacgagc
     2581 cgggcaggaa cacgtgcgcg tagcgggccg tctcgttcag gaagatgtcc tgcacgacca
     2641 ggcattccag cgccgacagc gccgcggcca cgtgctgcgt gtccgggtcc gactgcacca
     2701 ggtcctcgcc ctggcagtac agacccttga agctgccggt cagcgccgcc tcgaacatgt
     2761 tggggatgcg cagtccgggc tcgggcagga tcgcgacacc ccacgcggcc tcgaatgcgc
     2821 cgcgcaccga ggtatccgac acgtgccggt accccggcaa ctcgtgcggg aacgatccca
     2881 tgtcgcatga gccctgcacg ttgttctggc cgcgcagcgg attgacgccc acgccttcgc
     2941 ggccgaggtt gcccgtggcc atcgccagat tggcgatcgc catcacggcg gtcgagccct
     3001 gcgcgtgctc gctgacgccg agcccgtaat agatcgccgc attgccgccg gtcgcgtaca
     3061 gccgcgccgc accgcgcacg agcgcggcgg gcacgcccgt gtgcgcctcg atccgttcgg
     3121 gcgagttctc cgggcgcgcg atgaactcgc gccactgcgc gaacgccgtc ggctcgcagc
     3181 gcgcggcgac gaacgattcg tccaccagcc cctcggtgac gatcacgtgc gccagcgcat
     3241 cgagcatcgc gacattggtg ccgggtttga gctgcaggtg gtagtcggcc ttcacgtgcg
     3301 caccactgac cagttcgatc tgccgcggat cgatcacgat cagctgcgcg ccctggcgca
     3361 gccgccgctt cagccgcgag gcaaataccg ggtgggcgtc ggtcgggttg gcgccgatca
     3421 ccacgatcac gtcggcgtgc tcgaccgact tgaaggtctg cgtgccggcc gattcgccga
     3481 gagtctgctt cagcccgtag ccggtcggcg aatggcagac gcgcgcgcag gtatcgacgt
     3541 tgttgttgcc gaacgcggcg cgcaccagct tctgcaccag gtacgtctct tcattggtgc
     3601 agcggctcga agtgatcccg ccgatcgaat cgcggccata ttgcgcctgg atttcgcgca
     3661 agcgccgcgc ggcatgcgaa atcgcctcgt cccagctcac ctcgcgccac gggtcggtga
     3721 accggtcgcg gatcatcggc ttcgtcacgc gatccgggtg cgtcgcgtag ccccatgcaa
     3781 agcgaccctt cacgcaggcg tgtccctcgt tggcggcgcc gtgcctgtcc ggcaccatgc
     3841 gcacgaccgc gtcgtccttc aactcggcct tgaagctgca gccgacgccg cagtacgcgc
     3901 acgtcgtgat cgcagtgcgg tccggcgcgc cgagcccgag caccgatttc tcgcgcagcg
     3961 agccggtcgg gcacgcggcg acacacgcgc cgcaagacac gcattcggat tgcatgaacg
     4021 gcaggccgcc cgcgctgatg cgcgatccaa agccgcggcc gtcgaccgtc agcgcgtagg
     4081 tgccctgcgt ctcctcgcac gcgcgcacgc agcggttgca gacgatgcac agggcgggat
     4141 cgaacgcgaa atacgggttc gaggcgtcaa ccggctcggc tccgtgatgg gcgccgctcg
     4201 agtagcgaac gtcgcgcacg ccgaggtcgt cggccagccg gccgaactgc gtttcccggt
     4261 cgatgggtcg gccgtccgtg tcgcgcggat gatcggagag atacagctcc atcacgccgc
     4321 gccgcaggcg cgacagcttg tcgctctgcg tgcgaacgcg catgccggct tcgacgggcg
     4381 tggtgcacga tgcgggggtg cccttgcggc cctcgatctc gaccaggcac agccggcacg
     4441 agccccacgc ctcgaggctg tcggttgcgc acagcagcgg gatctcgatg ccggcctcga
     4501 tcgcggcgcg cagcaccgac gtgccggccg gcaccgtgac cgcacggccg tcgatggcga
     4561 gcgtgaccgg actgccggtg cgcgcgggcg tgccgtagtc gatgtcgtcg cgggttcgcc
     4621 taagtgcggt ggcctgcatc tgcgactccc tcaccgaagt cctgaggata atggttcagc
     4681 gccgacagca ccgggtaggg cgtcatgcca cccatcgcgc acagcgaccc gtcgaccatc
     4741 gtttcgcaca gttcgcgcag cagtgcgatc cgttgcggcc ggccgcggcc ggcgatgatg
     4801 tcgtcgatca gctccacgcc gcgcaccgag ccgactcggc acggcgtgca cttgccgcat
     4861 gactcgaacg cacagaattc catcgcgaaa cgcgcgagct gcgccagatc tgcagtgtcg
     4921 tcgtgcacga ccacgctgcc atggccaagc accgcgccga tttccgcaaa gcgttcgtag
     4981 tcgagcggta tgtcccagtg gtgttcgggg atgtacgcgc cgagcggccc gcccacctgc
     5041 accgcgcgga tgggccgacc cgacgcggtg ccgccgcccc aggtcgtcag cagttcgcgc
     5101 aacgtgatgc cgaatgcggc ttcgatcagt ccgccgcgcc gaatgttccc ggcgagttgc
     5161 acggtcagcg tgccgcggga gcgtccgacg ccgtagtcgc ggtagaacgc ggcgccgcgc
     5221 gcaaggatga tcggcacggt cgcgagcgtg accacgttat tgatcacgtt cggccggccg
     5281 aacaggcccg cgatcgccgg cagcggcggc ttggcgcgca ccacgccgcg gcggccctcg
     5341 aggctttcga gcatcgcggt ttcttcgccg cacacg
//
LOCUS       DBNHBY010000184        18091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_348545, whole genome shotgun sequence.
ACCESSION   DBNHBY010000184 DBNHBY010000000
VERSION     DBNHBY010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_348545"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="metX"
                     /locus_tag="AC16HB_04475"
     CDS             <1..1076
                     /gene="metX"
                     /locus_tag="AC16HB_04475"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR5606078.1"
                     /translation="LASGASIGDYMLLYETYGELNAARSNAVLICHALNASHHVAGVY
                     ADQPKNVGWWDNMVGPGKPVDTNRFFVVGVNNLGSCFGSTGPMSVNPATGRPYGASFP
                     VITVEDWVDAQARLADALGIERWAAVMGGSLGGMQAISWAIRYPARIAHSVAIACTPK
                     LTAQNIAFNEVARTAIRSDPDFHGGDYYAHGVVPKRGLTVARMIGHITYLSGDDMAEK
                     FGRSMRTDDYQFHYDVEFEVESYLRYQGDKFSEYFDANTYLLITRALDYFDPARATGG
                     DLRAALAPARAQFLLASFTTDWRFTPARSREIVEALIANDRDVTYAEIDAPHGHDAFL
                     LDDPRYHAAVAAYFDNIAREVGA"
     gene            1073..1657
                     /gene="metW"
                     /locus_tag="AC16HB_04480"
     CDS             1073..1657
                     /gene="metW"
                     /locus_tag="AC16HB_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR5606079.1"
                     /translation="MTLRADLQLIAGWIAPATRVLDLGCGDGALLAHLRDGQRCRGYG
                     VEIDDGHVLDCIRRRVNVIQSNLDAGLRMFSDDMFDIVVLSQTLQAMHRVEDVLREMA
                     RVARHGIVSFPNFGHWRHALALLGGHMPVTRAIPYQWYDTPNIHLCTPKDFEILAAKL
                     GFSIKQRALLADGAPVEFAPSLRATLAVYRFDMR"
     gene            1805..2308
                     /locus_tag="AC16HB_04485"
     CDS             1805..2308
                     /locus_tag="AC16HB_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5606080.1"
                     /translation="MSLRDKLVRNIEEVALEDFRREPRYDTRCGGVIGPRVPGTLARD
                     LGAGYDILAPGKISCPYHSHYAEEEMFVILEGEGTLRVAGEMIPIRAGDVIDIPPGPE
                     YPHQIINSSSAPLRYLSISTQKSPEVCEYPDSGKYGLYTREKGGIVAGRRLHRAETDL
                     DYWDGEP"
     gene            complement(2287..3720)
                     /locus_tag="AC16HB_04490"
     CDS             complement(2287..3720)
                     /locus_tag="AC16HB_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR5606081.1"
                     /translation="MQVRAVANTVTSNIVVAARVRGPRAWLQALAVYREPAVLRMLFL
                     GFSAGLPLLLVFGTLSFWLREAGVDLTTIGFVSWVALAYAFKWAWAPLVDRLPLPWLT
                     RTLGRRRSWLLVSQCAVAASLVGMALTDPARNLHLLVVLALLTAFASATQDIALDAYR
                     IESAAVEKQAALAAAYQTGYRIAMIWAGAGALAIAARIDPGAGGYSFAAWTAAYLAMA
                     GSMVVGVITTLISPEPAARVSTDTLAREQAAAVQISAHAHSPRQARLLAWLHGAVVAP
                     FADFLRRYRWHAVLLLALIATYRISDIVMGVMANPFYKDMGFSKDEVAAVSKIYGVVM
                     TLAGAFVGGVISLRIGVLRTLFLGAALSALTNLLFAWLALRGHDLTFLVFAISADNLS
                     AGIASSAFVAYLSGLTNVAYSATQYALFSSVMLLLPKYLAGFSGLFVDSFGYVNFFVA
                     TALLGLPVLLLVWLAARAAQAQGSPSQ"
     gene            3828..4643
                     /locus_tag="AC16HB_04495"
     CDS             3828..4643
                     /locus_tag="AC16HB_04495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5606082.1"
                     /translation="MRSWRSTAIALLLAVAPALQAQTPTDPAVQVRKPRLMLVPESTV
                     NQSAAQQYAQLKQQAAARGVLNTDVPNVQRVQAIAKRLIPHGARINPRAADWRWEVNV
                     IDAPTVNAFCMPGGKIMVFDGIIDRLQLNDNELAAVMGHEMAHALLEHGRARMSEAVM
                     KNVGVNLAAAYFGLSSVGTQALAQAAEVAISLPYSRGHESDADLLGIELAARAGYDPR
                     AAVTLWQKMAAASRGQPPQFLSTHPGHAERIRKIEAALPRVMPLYEAAQRPTR"
     gene            complement(4644..5420)
                     /locus_tag="AC16HB_04500"
     CDS             complement(4644..5420)
                     /locus_tag="AC16HB_04500"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR5606083.1"
                     /translation="MFRIATLNVNGIRSAAAKGLFRWVRRADPDVLCLQEVKAHEQAI
                     PERLRYHDGLTSTFYCAQKKGYAGTALYSKKKPRRVQLGIGVAEFDDEGRYIEAEFPG
                     VTVISVYFPSGSAGPARQASKDRFLDVFYPHLERLRAGGREVIFCGDVNIAHREIDLK
                     NWRSNQKNSGFLPHERAWLSRVFDDQGWVDVFRRLDPRPDRYTWWSNRGQAWSKNVGW
                     RIDYQIATPGIAAKAKAAEIYTKQRFSDHAPLIIDYAGRL"
     gene            5498..6175
                     /gene="pyrE"
                     /locus_tag="AC16HB_04505"
     CDS             5498..6175
                     /gene="pyrE"
                     /locus_tag="AC16HB_04505"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR5606084.1"
                     /translation="MDLLRRQFIEFALQSGVLRFGEFTTKAGRKSPYFFNAGLFCRGS
                     TLGRLARFYAQTLVAAQAAGRVRFDMLFGPAYKGITLASSAAVALAELGHDIGFAFNR
                     KEAKHHGEGGVIVGTPLAGRVMIVDDVITEGLAKRESVQLIRSLGAEPAGVVIALDRR
                     ERTGPDDKLSSQSAVQAFEQAFGVPIVAIADFDDLIDFLDSGSPLAVPAQPYRAAMQA
                     YRSRYGV"
     gene            6225..6845
                     /locus_tag="AC16HB_04510"
     CDS             6225..6845
                     /locus_tag="AC16HB_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606085.1"
                     /translation="MTDATAATGTGSGNLYVCTDAHGKTITSDQPPPECADRPIRELR
                     NDGSLKRVIEPPLNAAQREQKAAEDRRKAEEEGRRRDQARRDKALLDAYSDEKEIEDA
                     RTRAVDGRRVIIDRARKRMDELQRERKKLDDEAEFYAKRDKPEKLKRAYDTNATMVKA
                     QEKIIADTEAELKRINERFDADLKRYRQLVQTGGKPAPRSASTASR"
     gene            complement(6853..8301)
                     /gene="gatB"
                     /locus_tag="AC16HB_04515"
     CDS             complement(6853..8301)
                     /gene="gatB"
                     /locus_tag="AC16HB_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5606086.1"
                     /translation="MAWEVVIGLETHAQLSTRSKIFSGASTAFGAAPNTQACAVDLAL
                     PGVLPVLNRGAVERAIKLGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQG
                     GALSFVVKPKKGEPYTKTVRLTRAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVS
                     EPDLRSSDEAVAYAKALHALVVWLGICDGNMQEGSFRCDANVSVRPLGQKEYGTRCEI
                     KNLNSFKFLQAAIDYEVRRQIELIEDGGTVVQETRLYDPERDETRSMRSKEDAMDYRY
                     FPDPDLLPLAISKEWIEQVAREMPELPAAMRERFVRDYALPEYDAGVLTSSKAMAAYF
                     EEVARAVPDKKAAANWVMGEVAAALNDAAIEIDAAPVSATQLAALIGRVLDGTVNNKT
                     AKEVFAALWSRAGESADAIIEARGLKQISDAGAIEKLIDEVLAANAKSVDEFKAGKEK
                     AFNALVGQCMRATKGKANPQQVNEILRRKLGG"
     gene            complement(8303..9775)
                     /gene="gatA"
                     /locus_tag="AC16HB_04520"
     CDS             complement(8303..9775)
                     /gene="gatA"
                     /locus_tag="AC16HB_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR5606087.1"
                     /translation="MSELAHASVAELGRLLEDRTVSAVELARLFLERIDAHRHLNAFL
                     DVRPEVTLAQARAADARRAKGEAGPLLGVPIAHKDIFATRDWATTAGSKMLRGYMSPF
                     NATVVDNLARAGMVCLGKLNCDEFAMGSSNENSAYGNVLNPWDTRAVPGGSSGGSSAV
                     VAGRIAPVATGTDTGGSIREPAAFTGVTGLKPTYGRASRWGVVAFASSLDHPGLFAQS
                     ARDAALVCNAMLGFDPKDSTSVDRPAEDFSRHLNMPVQGLRIGVPQEFFGAGLQPDVE
                     AAVRGALADYERMGARLVDVRLPNADLGIPVYYVIAPAEASSNLSRFDGVRYGHRAEK
                     YADLIDMIKKSRSEGFGAEPKRRILIGTYVLSHGYYDAYFIKAMQVRRLIADDFQRAF
                     RECDVIAGPVTTSVAFEFGEKSADPVEMYMSDLYTIPADLVGVPALSIPCGFGQGEKN
                     AHRPVGLHLMTNHFEEAKLLGVAAAYQRATDWHQRIPAGF"
     gene            complement(9786..10073)
                     /gene="gatC"
                     /locus_tag="AC16HB_04525"
     CDS             complement(9786..10073)
                     /gene="gatC"
                     /locus_tag="AC16HB_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5606088.1"
                     /translation="MSLTIDDVRRIAHLARIEITDTQAEATLAQLNDIFAMIEKMQQV
                     PTAGVEPMSHPLGGSQRLRDDVATEGDERDANLRNAPEQQDGLFLVPKVIE"
     gene            10279..11322
                     /locus_tag="AC16HB_04530"
     CDS             10279..11322
                     /locus_tag="AC16HB_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR5606089.1"
                     /translation="MFGFLRSYFSSDLAIDLGTANTLIYVRGKGIVLNEPSVVAISQE
                     GGPSGKRTIRAVGREAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPS
                     SLFAPSPRIIICVPCGSTQVERRAIKESAEAAGASQVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYAGSVRVGGDKFDESIINYIRRNYGMLIGEPTAES
                     IKKEIGSAFPGSEVKEMEVKGRNLSEGIPRSFTISSNEILESLTDPLNQIVSAVKIAL
                     EQTPPELGADIADRGMMLTGGGALLRDLDRLLMEETGLPVLVAEDPLTCVVRGCGIAL
                     ERMDKLGTIFAQG"
     gene            11360..12253
                     /gene="mreC"
                     /locus_tag="AC16HB_04535"
     CDS             11360..12253
                     /gene="mreC"
                     /locus_tag="AC16HB_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR5606090.1"
                     /translation="MEHGPPPLFNQGVSARARLAFFSFLAIALIIVDARVRALETIRV
                     GVGVVLYPIQQVLLAPVHMAEAVGEYFTTLSRLTRENEALKREAAERARELQQAKALE
                     SENAQLRRLLAAREKSGKPGVLAQVLYESRDRFTRKLALDKGSSDGLRTGQPVIDDRG
                     VLGQITRVFPLTAEMTLLTDKEQSIPIQIARNGLRGVAYGGSEPGTLDLRFMPANADV
                     KQGDIAMTSGIDAIYPAGLQVATVTKVERAENDQFSRIVMTPTAGVQNYAFLLVLPVD
                     ASAPPRPADETRSEPRKAVRK"
     gene            12411..12884
                     /gene="mreD"
                     /locus_tag="AC16HB_04540"
     CDS             12411..12884
                     /gene="mreD"
                     /locus_tag="AC16HB_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR5606091.1"
                     /translation="MFIWMSLLVALVINLMPWGQATFVPDFVALGLIFWNVHQPRRVG
                     IGAAFVFGLLMDVHNGALFGEHALAYTLLSYGAISLHRRILWFPLGAQALHVFGLLLV
                     AQFASMLVRLWVGGSFPGLSYFAESLVGALVWPIVTWLLLAPQRQPHERDETRPI"
     gene            complement(12891..13148)
                     /locus_tag="AC16HB_04545"
     CDS             complement(12891..13148)
                     /locus_tag="AC16HB_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606092.1"
                     /translation="MTHDQELLGLLQKIEANQRQALAAQTEHLKLAQEQFARSEARIE
                     ESLKIQRLAVQRQQRLSLAVLPVILGLIVLVVWIAIKWRVI"
     gene            complement(13152..13772)
                     /locus_tag="AC16HB_04550"
     CDS             complement(13152..13772)
                     /locus_tag="AC16HB_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5606093.1"
                     /translation="MFDIQHYPSFIAAVLVFQAVPGPGTLAILNATARNGIGAGMGAV
                     AGTLVGDFAYMLAAVLGLAAVMNANPMLFTALQWFGAGYLIWIGLQLLQARSPATHGD
                     AEPRRSAWVYFRQAFAVSLTNPKVMLFFVAFFPLFLRADSSPATLAAMMLHVTGLSLV
                     YQTALVLAGNAVAQRLAALPGARRLATRLAGIALIGFGAKLALSNR"
     gene            complement(13851..14171)
                     /gene="sugE"
                     /locus_tag="AC16HB_04555"
     CDS             complement(13851..14171)
                     /gene="sugE"
                     /locus_tag="AC16HB_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HIR5606094.1"
                     /translation="MSWVILVLAGLFEVGWAIGLKYTQGFTRPWPTVWTVAAMIASLW
                     LLGIAMRTLPVGTAYSVWVGVGAVGTVILGIVLFGEPASALRLGSVALIVAGIVGLKL
                     ATPS"
     gene            14402..16336
                     /gene="mrdA"
                     /locus_tag="AC16HB_04560"
     CDS             14402..16336
                     /gene="mrdA"
                     /locus_tag="AC16HB_04560"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5606095.1"
                     /translation="MTTFRNPEHEIHHFRTRVVAATLFVLVCFGALLARFVWLQVIKH
                     AELYEQAEINRIAVVPVQANRGLIRDRNGAIIARNFSAYTLEINPRKVENVNAVIDEL
                     AGVIEVQPRDRRRFKKLQEELKGAESIPIRTRLTDDEVARFAAQRFRFPGVELRARLF
                     RHYPLGEVGAHMIGYIGRISPADKERIATFEDTDNYVGAEHIGKTGIELSYQRELHGA
                     TGSERVEITAGGRAVRTLDRSPSTPGNNLNLSIDIKLQQIVEQAFGDRRGALIAIEPE
                     TGDVLAFVSKPSFDPNLFVDGIDPKNWDELNNDPDKPLLNRALRGTYPIGSTYKPFLA
                     LAALETGKRRPEQVIYDPGYFVFGNRRFRDSNKVPLGPVDMHKSIVKSSDIYYYMVAN
                     DLGVDTIHDFMKPFGFGQITGIDLEGEQTGVLPSSAWKEKRFKQKWFPGETISIGIGQ
                     GYNAFTLLQLAHATATLANDGVVMKPHLVKSVEDVKTGEVRLTVPKESYRIPLKPEHI
                     RFVREAMVDVNISGTGRQAFAGVPYKVAGKTGTAQVIGIKQNEKYNANRIDERHRDHS
                     LFIAFAPAGPGEKPKIALALLVENGGFGSMAAAPIARQAIDYYLLGKVPNAPAPLKPE
                     GDGEPSGELVAQVNGGAREQ"
     gene            16468..17586
                     /gene="rodA"
                     /locus_tag="AC16HB_04565"
     CDS             16468..17586
                     /gene="rodA"
                     /locus_tag="AC16HB_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR5606096.1"
                     /translation="MNSDTLRARIWLWVRERLSVLDPALLAIIGGLMLLGLITLYSAA
                     LDFPWRFTDQMRNFAIAAGVMFLVASIPPSVLMKAALPIYVIGLVLLVAVDRFGVIVK
                     GAQRWLDLGFTRIQPSEVMKLALPMMLAWYFHKREGAVRSLDFLLAALLLILPVGLIA
                     KQPDLGTAILVLAAGFFVIFFAGLPWKWLAAMGVAGAAMLPVAWTFMHGYQRERIMTL
                     IDPSQDPLGKGFHTLQAMIAIGSGGLTGKGWMQGTQTHLEFIPERTSDFIFAVYSEEF
                     GLIGNVVLVVLFFALVTRALLIAANAATIYARLMAAAIALIIFTYGFVNMGMVSGILP
                     VVGVPLPFVSYGGTALVTLGMACGMLMSIARNRKLVQS"
     gene            17583..>18091
                     /locus_tag="AC16HB_04570"
     CDS             17583..>18091
                     /locus_tag="AC16HB_04570"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR5606097.1"
                     /translation="MNSVGGRRSAVVGLLAAALLAGCATAPKPPQRAGGYYQNDGPPA
                     QVPADLANVPDAVPRVEPLHPYANRPYVALGKSYTPLAADVPFRQRGLASWYGRQFHG
                     NRTSSGEIYDMFAMTAAHPTLPIPSYARVTSVKSGASVIVRVNDRGPFKDDRIIDLSY
                     AAATKLGFA"
BASE COUNT         3013 a         6162 c         6098 g         2818 t
ORIGIN      
        1 cgctcgccag cggtgcttcg atcggcgact acatgctgct ctacgagacc tacggcgaac
       61 tcaacgccgc gcgcagcaac gccgtgctca tctgccacgc gctgaacgca tcgcatcacg
      121 tcgccggcgt ctatgccgat cagccgaaga acgtcggctg gtgggacaac atggtcggcc
      181 cgggcaagcc ggtcgacacc aaccgcttct tcgttgtcgg cgtcaacaac ctcggctcct
      241 gcttcggctc gaccgggccg atgtcggtca atcccgccac cggccggccg tacggtgcga
      301 gctttccggt gatcacggtc gaggactggg tcgacgcgca ggcgcggctc gccgacgcgc
      361 tgggcatcga gcgctgggcc gcggtgatgg gcggctcgct cggcggcatg caggcgatct
      421 cgtgggcgat ccgctacccg gcgcgcatcg cgcacagcgt ggcgatcgcc tgcacgccca
      481 agctcaccgc gcagaacatt gcgttcaacg aggtcgcgcg caccgcgatc cgctccgacc
      541 cagatttcca cggcggcgat tactacgcgc acggcgtggt gccgaagcgc ggcctcaccg
      601 tggcgcgcat gatcggccac atcacgtatc tgtcgggcga cgacatggcg gagaagttcg
      661 gccgcagcat gcgcaccgac gactaccagt tccactacga cgtcgagttc gaggtcgaga
      721 gctacctgcg ctaccagggc gacaagttct cggagtactt cgacgccaac acgtacctcc
      781 tgatcacgcg cgcgctcgac tacttcgatc cggcccgggc caccggcggc gacctgcgcg
      841 cggcgcttgc cccggcacgg gcgcagttcc tgctggcgtc gttcaccacc gactggcgct
      901 tcacgccggc gcgctcgcgc gagatcgtcg aggcgctgat tgccaacgat cgcgacgtca
      961 cctacgccga gatcgacgcg ccgcacggtc acgacgcgtt cctgctcgac gatccgcgct
     1021 accacgcggc ggtggcggcg tacttcgaca acatcgcgcg cgaggtcggc gcatgaccct
     1081 gcgcgccgac ctgcagttga tcgccggctg gatcgcgccg gccacgcgag tgctcgatct
     1141 gggctgcggc gacggtgcgc tgctggcgca cctgcgcgac gggcagcggt gccgcggcta
     1201 cggtgtggag atcgacgacg gccacgtgct cgattgcatc cggcgacgcg tcaacgtgat
     1261 ccagtccaac ctcgacgccg ggctgcgcat gttcagcgac gacatgttcg acatcgtcgt
     1321 gctgtcgcag acgctgcagg cgatgcatcg tgtcgaggac gtgctgcgcg agatggcgcg
     1381 cgtggcgcgc cacggcatcg tcagctttcc gaacttcggc cactggcgcc acgcgctggc
     1441 gctgctcggc ggccacatgc cggtgacgcg ggcgattccg taccagtggt acgacacgcc
     1501 gaacatccat ctgtgcacgc cgaaggattt cgagatcctc gccgccaagc tcggcttcag
     1561 catcaagcag cgcgcgctgc tggccgacgg cgcgccggtc gagttcgcgc ccagcctgcg
     1621 ggccacgctc gcggtgtacc gtttcgacat gaggtgatga gccatcgccc ggccgtcccc
     1681 gggaaggctt ggccggcccg tcgcggggcg cgacgggcgt tcgagcgatc gcagcccgcg
     1741 cagccgggct gggagccgct ctcgccccat caggggacag cgccgtaggc gccaaggggg
     1801 aaacgtgagc ctgcgtgaca agctggtgcg caacatcgag gaagtcgcgc tggaagactt
     1861 ccggcgcgaa ccgagatacg acacgcgctg cggcggcgtg atcgggccgc gcgttcccgg
     1921 gacgctcgca cgcgatctcg gcgccggcta cgacatcctg gcgccgggca agatctcgtg
     1981 cccgtaccac tcgcactacg ccgaggagga gatgttcgtg atcctcgagg gcgagggcac
     2041 gctgcgcgtc gcaggcgaga tgatcccgat ccgcgccggc gacgtgatcg acattccgcc
     2101 cggccccgag tacccgcacc agatcatcaa ctcgtcgagc gcgccgctgc ggtatctgtc
     2161 gatcagcacg cagaagagcc ccgaggtgtg cgaatacccg gattcgggca agtacggcct
     2221 gtacacccgc gagaagggcg gcattgtcgc tggccgccgc ctgcatcgcg ccgagaccga
     2281 tctcgactac tgggacggcg agccctgagc ctgtgcggcg cgtgccgcca gccacaccag
     2341 cagcagcacc ggaagcccga gcagcgcagt ggcgacgaag aagttcacgt agccgaagct
     2401 gtcgacgaac agaccggaga agcccgccag gtacttcggc agcagcagca tcaccgacga
     2461 gaacagcgcg tactgcgtgg ccgagtaggc gacgttggtc agccccgaca ggtaggccac
     2521 gaacgcgctg ctggcgatgc ccgcggacag gttgtcggcc gagatcgcga acaccaggaa
     2581 cgtcaggtcg tggccgcgca gcgcgagcca cgcgaacagc agattggtca gtgccgacag
     2641 cgccgccccg aggaagagcg tgcgcagcac gccgatgcgc agcgaaatga cgccaccgac
     2701 gaaggcgccc gccagcgtca tcacgacgcc gtagatcttc gacaccgccg ccacctcgtc
     2761 cttggaaaac cccatgtcct tgtagaacgg attggccatc acgcccatca cgatgtcgga
     2821 gatgcgatag gtcgcaatca gcgcgagcag cagcaccgcg tgccagcggt agcggcgcag
     2881 gaaatcggcg aacggagcca cgaccgcgcc gtgcagccag gccagcagcc gcgcctgccg
     2941 cggcgagtgc gcgtgcgcgg agatctgcac ggcggccgcc tgttcgcgcg ccagcgtgtc
     3001 ggtggacacg cgcgcggccg gctcgggcga tatcagcgtc gtgattacgc cgacgaccat
     3061 cgaccccgcc atcgccaggt aggccgccgt ccacgccgca aacgaatagc cgcccgcacc
     3121 ggggtcgatg cgcgcagcga tcgccagcgc accggcgccg gcccagatca tcgcgatgcg
     3181 atagccggtc tggtaagcgg cggcgagtgc agcctgcttc tcgaccgcag ccgactcgat
     3241 ccggtacgca tcgagcgcga tgtcctgcgt tgccgacgcg aacgcggtca gcagcgccag
     3301 cacaaccagc aggtgcaggt tccgggcggg atcggtcagc gccatgccga ccaggctggc
     3361 cgcaaccgcg cattgcgata ccagcagcca gctgcgccgc cggcccagcg tgcgtgtgag
     3421 ccacggcagc ggcagccggt cgaccagcgg cgcccacgcc cacttgaacg cgtacgcgag
     3481 cgcaacccag ctgacgaatc cgatcgtggt cagatcgacg ccggcttcgc gcagccagaa
     3541 cgacagcgtg ccgaacacca gcagcagcgg cagcccggcc gagaagccga ggaacagcat
     3601 gcgcagcacc gccggctcac ggtacacggc gagcgcctgc agccaggcgc gcgggccgcg
     3661 tacgcgcgcc gcgacgacga tattcgacgt cacggtgttg gccactgcgc ggacttgcat
     3721 cgggtcgaag cgcccatatg ttggcgaatc agtgtagcgg ctgaactcgc acgaagtcct
     3781 tcggccgaac cgccggttaa gctcgcgcac agatggaggg aacaccgatg cgttcatggc
     3841 gatcgaccgc gattgcgctg ctgctggcgg tggcaccggc gctgcaggcg cagacgccga
     3901 ccgaccctgc cgtgcaggtg cgcaagccgc ggttgatgct ggtgcccgag tcgacggtca
     3961 atcaaagcgc ggcacagcag tacgcgcagc tgaagcagca ggcggccgcg cgcggcgtgc
     4021 tcaacaccga cgtgccgaac gtgcagcgcg tgcaggcgat tgcgaagcgg ctgatcccgc
     4081 acggcgcgcg catcaatccc cgcgcggccg actggcgctg ggaggtcaac gtgatcgacg
     4141 cgcccacggt caacgcgttc tgcatgccgg gcggcaagat catggtgttc gacggcatca
     4201 tcgaccggct gcaactgaac gacaacgaac tggccgcggt gatggggcac gagatggcgc
     4261 acgcgctgct cgagcacggc cgtgcgcgca tgagcgaggc ggtcatgaag aacgtcggcg
     4321 tcaacctcgc cgccgcgtac ttcggtctga gcagcgtcgg cacgcaggcg ctggcccagg
     4381 cggcggaggt ggcaatctcg ctgccgtatt cgcgtggcca cgaatccgac gccgatctgc
     4441 tcggcatcga actcgcggca cgcgcgggct acgacccgcg tgccgccgtc acgctatggc
     4501 agaagatggc ggccgccagc cgcggccagc caccgcagtt cctgtccacg cacccgggtc
     4561 acgcggagcg catcaggaag atcgaggcgg cgctgccgcg cgtgatgccg ctgtacgaag
     4621 cggcgcagcg gccgacacgc tgatcacagg cgcccggcgt aatcgatgat cagcggcgcg
     4681 tggtcgctga agcgctgctt cgtgtagatc tccgccgcct tggccttcgc cgcgatgccg
     4741 ggcgtggcga tctggtagtc aattcgccag ccgacgttct tggaccacgc ctggccgcgg
     4801 ttgctccacc atgtgtaacg gtccggtcgc gggtccagcc gccggaacac gtcgacccaa
     4861 ccctggtcgt cgaagacgcg cgagagccac gcccgctcgt gcggcaggaa acccgagttc
     4921 ttctggttcg agcgccagtt cttcagatcg atctcccggt gcgcgatgtt cacgtcgccg
     4981 cagaagatca cttcgcggcc gccggcccgc agtcgttcga gatgcggata gaacacgtcg
     5041 aggaagcggt ccttcgacgc ctgccgcgcg ggcccggccg agccggaggg gaagtagacc
     5101 gagatcaccg tgacgcccgg gaactcggct tcgatatagc ggccctcgtc gtcgaactcc
     5161 gccacgccga tgccgagttg cacgcgccgg ggcttcttct tcgagtaaag cgccgtgccg
     5221 gcgtagccct tcttctgcgc gcagtagaag gtgctggtca ggccgtcgtg gtagcggagt
     5281 cgttccggga tggcctgctc gtgcgccttg acctcctgca ggcacagcac gtcggggtcg
     5341 gcccgccgga cccagcggaa caggcccttg gcggcggcgg agcggatgcc gttgacgttc
     5401 agggtggcga tacggaacac cgggattagt ccgatcggtc agccgacacg atagcggctt
     5461 tttgacacaa ttcgcgccag tgcaactgct tacgtccatg gacctgctgc gccgacagtt
     5521 catcgagttt gccctgcagt cgggcgtgct gcgctttggc gaattcacga ccaaggccgg
     5581 gcgcaagtcg ccatacttct tcaatgccgg gctgttctgc cgcggttcca ccctcgggcg
     5641 gctggcgcgc ttctatgcgc agacgctggt cgccgcgcag gccgccgggc gcgttcgctt
     5701 cgacatgctg ttcgggcccg cttacaaggg catcacgctg gcatcgagcg cggcggtggc
     5761 gctggccgaa ctcggtcacg acatcgggtt cgcgttcaac cgcaaggaag ccaagcatca
     5821 cggcgagggc ggcgtgatcg tcggcacgcc attggccggc cgcgtgatga tcgtcgacga
     5881 cgtgatcacc gaggggctcg ccaagcgcga gtcggtgcag ctgatccgct cgctcggagc
     5941 cgagccggcc ggcgtggtga tcgcgctcga ccggcgcgag cgtaccgggc ccgacgacaa
     6001 gctgtcatcc cagtcggcgg tccaggcgtt cgagcaggca ttcggcgtgc cgatcgtggc
     6061 aattgcggac ttcgacgatc tgatcgactt cctcgattcc ggctcgccgc tggccgtacc
     6121 ggcgcagccg taccgcgcgg cgatgcaggc ctatcggagt cgctatggcg tctgatctgc
     6181 gtttgggtgc ggcgtgcgtg gtcatcgcgg ccggtgtcgg ggccatgacc gatgccacgg
     6241 cggcgaccgg caccggcagc ggcaacctgt acgtgtgcac cgatgcgcat ggcaagacga
     6301 tcacgtcgga tcagccgcca ccggaatgtg cggaccggcc gatccgtgaa ctgcgaaacg
     6361 acggctcgct gaagcgcgtg atcgagccgc cgctgaacgc cgcgcagcgc gaacagaagg
     6421 ctgccgagga tcggcgcaag gccgaggagg aaggacgccg gcgcgaccag gcgcggcgcg
     6481 acaaggcgct gctcgacgcc tacagcgacg aaaaggagat cgaggacgcg cgcacgcgcg
     6541 ccgtcgacgg ccggcgcgtg atcatcgacc gcgcccgcaa gcggatggac gaactgcagc
     6601 gcgagcgcaa gaagctcgac gacgaagccg agttctacgc caagcgcgac aagccggaga
     6661 agctgaagcg cgcctacgac accaacgcga cgatggtgaa ggcgcaggaa aagatcatcg
     6721 ccgataccga ggcggaactg aagcgcatca acgagcgctt cgacgccgac ctcaagcgct
     6781 accgccaact ggtgcagacc ggcggcaagc cggcgccgcg cagcgcgagc accgcgtcgc
     6841 gctgagcgct cctcagccgc ccagcttgcg gcgcaggatc tcgttgacct gctgggggtt
     6901 cgccttgccc ttggtcgcgc gcatgcactg gccgaccagc gcgttgaatg ccttctcctt
     6961 gccggccttg aactcgtcga cggatttcgc gttggcggcg agcacctcgt cgatcagctt
     7021 ctcgatcgca ccggcgtcgg agatctgctt cagcccgcgc gcctcgatga tcgcatcggc
     7081 gctttcgccc gcgcggctcc atagcgcggc gaacacttcc ttggcggtct tgttgttgac
     7141 cgtgccgtcg agaacgcgac cgatcagagc ggcaagctgt gtggccgaca ccggcgcggc
     7201 atcgatctcg atcgcagcgt cgttcagcgc ggcggcgacc tcgcccatga cccagttggc
     7261 ggcggccttc ttgtccggca ccgcgcgcgc gacctcctcg aagtaggccg ccatcgcctt
     7321 cgaactcgtc agcacgccgg cgtcgtactc cggcagcgcg tagtcgcgca cgaagcgctc
     7381 gcgcatcgcg gcaggcaact ccggcatctc gcgtgcgact tgctcgatcc actctttcga
     7441 aatcgcgagc ggcagcaggt ccggatcggg gaagtagcga tagtccatcg cgtcttcctt
     7501 gctgcgcatg ctgcgcgtct cgtcgcgctc ggggtcgtaa agccgggtct cctgcacgac
     7561 cgtgccgcca tcctcgatca gctcgatctg ccgccgtacc tcgtagtcga tcgcggcctg
     7621 caggaacttg aagctgttca ggttcttgat ctcgcagcgc gtgccgtatt ccttctgccc
     7681 gagcggtcgc acgctgacgt tggcatcaca gcggaagctg ccctcctgca tgttgccatc
     7741 gcagatgccg agccacacga ccagcgcatg cagcgccttc gcgtacgcga ctgcctcgtc
     7801 ggaggagcgc aggtcgggct cggagacgat ctccagcagc ggcgttccgg cgcggttcag
     7861 atcgatgccg gtcatgccgt gatagtcctc gtgcagcgac ttgccggcgt cttcctcgag
     7921 gtgcgcgcgc gtcagccgca cggtcttcgt gtaaggctcg cccttcttcg gcttgaccac
     7981 gaacgacagc gcaccgccct gcacgacggg gatctcgtac tggctgatct ggtagccctt
     8041 cggcaggtcc gggtagaagt agttcttgcg cgcgaagatc gagcgcggcg caacggtggc
     8101 gccgatcgcc agcccgagct tgatcgcgcg ctcgaccgcg ccgcggttca ggaccggcag
     8161 cacgccgggc agcgccagat cgaccgcgca cgcctgcgtg ttgggcgcgg cgccgaacgc
     8221 ggtcgaagcg cccgagaaga tcttgctgcg cgtcgacagt tgcgcgtgcg tttcgagccc
     8281 gatgacgact tcccacgcca tgtcagaacc ccgccgggat gcgctggtgc cagtcggtcg
     8341 cgcgctgata cgccgctgcg acgccgagca gcttcgcttc ctcgaaatgg ttcgtcatca
     8401 ggtgcagccc gaccggccgg tgcgcgttct tttcgccctg cccgaagccg cacgggatcg
     8461 acagcgccgg cacgccgacg aggtcggccg ggatcgtgta caggtcggac atgtacatct
     8521 ccaccgggtc ggcggacttc tcgccgaact cgaacgccac gctggtcgtc accggaccgg
     8581 cgatcacgtc gcactcgcgg aacgcgcgct ggaagtcgtc ggcgatcagc cgccgcacct
     8641 gcattgcctt gatgaagtag gcgtcgtagt agccgtgcga cagcacgtag gtgccgatca
     8701 ggatgcgccg cttcggctcg gcgccgaagc cctcggagcg cgatttcttg atcatgtcga
     8761 tcaggtcggc gtacttttcg gcgcgatgcc cgtagcgcac gccgtcgaag cgcgacaggt
     8821 tcgagctcgc ctccgccggc gcaatcacgt agtacacggg aatgccgagg tcggcgttcg
     8881 gcagccggac atcgaccagt cgcgcgccca tccgttcgta gtcggcgagc gcgccacgca
     8941 ccgcggcttc gacgtccggc tgcagtccgg cgccgaagaa ttcctgcggc acgccgatcc
     9001 tcagcccctg cacgggcatg ttcaagtggc gcgagaagtc ctcggccgga cgatcgacac
     9061 tggtcgaatc cttcggatcg aagcccagca tcgcgttgca gacgagcgcc gcgtcccggg
     9121 ccgactgcgc gaacaggccc ggatgatcga ggctggaggc gaatgccacc acgccccagc
     9181 gcgacgcgcg cccgtacgtg ggcttcaggc ccgtgacgcc ggtgaatgct gccggctcgc
     9241 ggatcgatcc gccggtgtcg gtgccggtgg ccaccggtgc gatgcgtccg gcgacgaccg
     9301 ccgacgaacc gcccgaggac ccgcccggca cggcgcgcgt gtcccacggg ttcagcacgt
     9361 tgccgtatgc gctgttctcg ttgctcgagc ccatcgcgaa ctcgtcgcag ttcagcttgc
     9421 caaggcacac catgccggct cgcgcgagat tgtcgacgac ggtcgcgttg aacgggctca
     9481 tgtaaccgcg cagcatcttc gagcccgcgg tggttgccca gtcgcgcgtg gcgaagatgt
     9541 ccttgtgcgc gatcggcacg ccgagcagcg gcccggcctc gcccttcgcg cgccgcgcgt
     9601 ctgccgcgcg cgcctgcgcc agcgtgacct ccggccgcac gtcgagaaac gcgttcaggt
     9661 ggcggtgcgc gtcgatgcgc tcgaggaaca gccgcgccag ctcgaccgcc gacaccgttc
     9721 tgtcctcaag cagccgtccc agttccgcga cggacgcgtg cgccagttcg ctcatggctg
     9781 cccggctatt cgatcacctt cggcacgagg aacagcccgt cctgctgctc gggcgcgttg
     9841 cgcaggttgg cgtcgcgttc atcgccctcg gtggcgacat cgtcgcgcag gcgctggctg
     9901 ccgcccaggg gatgcgacat cggctcgacg cctgcggtcg gcacctgctg catcttctcg
     9961 atcatcgcga agatgtcgtt caactgcgcc agcgtcgctt ctgcctgcgt gtcggtgatc
    10021 tcgatgcgcg ccaggtgcgc aatgcggcgg acgtcgtcga tggtcagaga catcggagcg
    10081 cggaaccggg cgggacagac gaagggatga gcgaaaaaat gtggtcgcgg gagtgaaatc
    10141 acacgcgcgt cggagtgacg actcgcattc ctagtctatc atccgagcct catcgcttct
    10201 ctctggcgca agccatttcg agcaacgtct gcgccgccgc ccacggccgc agcgcagcga
    10261 ggatcagaca aggcgtccat gttcggcttt ctccgcagct acttctccag cgatctcgcg
    10321 atcgacctcg gcactgccaa caccctcatc tacgtccgcg gcaagggcat cgtcctgaac
    10381 gagccgtcgg ttgtcgccat cagccaggaa ggcggcccca gcggcaagcg cacgatccgc
    10441 gcggtcggcc gcgaggccaa gcagatgctc ggcaaggtgc cgggcaacat cgaagcgatt
    10501 cgcccgatga aggacggcgt gatcgccgac ttcacggtca ccgagcagat gctcaagcag
    10561 ttcatcaaga tggtgcaccc gtcgagcctg ttcgcgccca gtccgcgcat catcatctgc
    10621 gtgccgtgtg gctccacgca ggtggagcgg cgcgcgatca aggaatcggc cgaggcggcc
    10681 ggagcatcgc aggtctatct gatcgaggag ccgatggccg ccgcgatcgg cgccgggctg
    10741 ccggtgtcgg aggcgtctgg ctcgatggtg gtcgacatcg gcggcggcac gacggaggtc
    10801 ggcgtgatct cgctcggcgg catggtgtac gcgggcagcg tgcgcgtcgg cggggacaag
    10861 ttcgacgagt cgatcatcaa ctacatccgc cgcaactacg gcatgctgat cggcgagccc
    10921 accgccgagt cgatcaagaa ggagatcggt tccgcgttcc cgggctccga ggtcaaggag
    10981 atggaggtca aggggcgcaa cctctccgag ggtattccgc gcagtttcac gatctccagc
    11041 aacgagatcc tcgaatctct gaccgacccg ctgaaccaga tcgtctcggc ggtcaagatc
    11101 gcgctcgagc agacgccgcc ggaactcggc gcggacatcg ccgaccgcgg catgatgctg
    11161 accggcggcg gcgcgctgct gcgcgacctg gaccgcctgc ttatggaaga gacaggactg
    11221 ccggtgctgg tggccgagga tccgctgacc tgcgtcgtgc ggggctgcgg catcgcgctg
    11281 gagcgcatgg acaagctcgg cacgatcttc gcgcagggct gatgccaccg gcgctgcagg
    11341 ccttgcgccc cggagacgga tggaacacgg cccgccgccg ctattcaacc agggcgtttc
    11401 cgcccgcgcg cggctcgcat tcttctcctt tcttgcgatc gcgctgatca tcgtcgacgc
    11461 gcgcgtgagg gcgctcgaaa cgatccgcgt cggcgtcggc gtggtgctgt atccgatcca
    11521 gcaggtgctg ctcgcgccgg tgcacatggc cgaagcggtc ggcgaatact tcacgacctt
    11581 gagccggctg acgcgcgaaa acgaggccct gaagcgcgag gccgccgagc gcgcgcgcga
    11641 actgcagcag gccaaggcgc tggaatccga gaatgcgcaa ctgcggcgcc tgctggccgc
    11701 gcgcgagaag agcggcaagc cgggcgtgct agcgcaggtg ctgtacgagt cgcgcgatcg
    11761 gttcacgcgc aagctggcgc tcgacaaggg cagcagcgac gggctgcgca ccggccagcc
    11821 ggtgatcgac gatcgcggcg tgctcgggca gatcacgcgc gtgttcccgt tgacggcgga
    11881 gatgacgttg ctcacggaca aggagcagtc gattccgatc cagatcgcgc gcaacgggct
    11941 gcgcggcgtg gcctacggtg gttcggagcc cggcacgctc gacctgcgct tcatgcccgc
    12001 caatgccgac gtcaagcagg gggacatcgc gatgacctcc ggcatcgacg cgatctaccc
    12061 ggccggactg caggtggcta cggtgaccaa ggtcgaacgc gcggagaacg accagttctc
    12121 gcgcatcgtg atgacgccga cggcaggagt gcagaactac gcgttcctgc tggtgctgcc
    12181 ggtcgacgcg tcggccccgc cgcgcccggc ggacgagacc cggtccgagc cgcgcaaggc
    12241 cgtccgcaaa tgatgggctg cgggcgatga gctggttcaa gcgcctgcgc agcgccttcg
    12301 cgctgaaccg tccgcgcaac ccggaccccg tcgaactcgt ttcggccggc ggcttgagcg
    12361 tcggccgcgc cgcgtatcga ccgtcggtgc tgctgcgccc cgccaacccg atgttcatct
    12421 ggatgtcgct gctggtcgcg ctggtgatca acctgatgcc gtggggacag gccaccttcg
    12481 tgccggactt cgtggcgctc ggactcatat tctggaacgt gcaccagccg cgccgggtcg
    12541 gcatcggcgc ggcgttcgtg ttcgggctgc tgatggacgt gcacaacggt gcgctgttcg
    12601 gcgagcatgc gctggcctac acgttgctgt cgtacggcgc gatctcgctg caccggcgca
    12661 tcctgtggtt cccgctcggc gcgcaggcgc tgcacgtgtt cgggttgctg ctggtggcgc
    12721 aattcgcgtc gatgctggtc aggctctggg tcggtggcag cttcccggga ctgagctact
    12781 ttgccgaaag cctggtcggc gcgctcgtat ggccgatcgt cacctggctg ctgctcgcgc
    12841 cgcagcgcca gccgcacgaa cgcgacgaga cgcggccgat ctagcgcgtc tcagatgacg
    12901 cgccacttga tcgcgatcca gaccaccagc acgatcagcc ccaggatcac cggcagcacc
    12961 gccagcgaca gccgctgctg acgctgcaca gccaagcgct gaatcttcag cgactcctcg
    13021 atccgcgcct cggaacgcgc gaactgctcc tgcgccagtt tcagatgctc ggtctgcgcc
    13081 gcgagggcct ggcgctggtt cgcttcgatc ttctgcagca ggccgagcag ttcctgatcg
    13141 tgtgtcatgg atcaccggtt gctaagggcc agctttgcgc cgaaaccgat cagcgcgatc
    13201 cccgccagtc gcgtcgccag ccggcgtgct cccggcaaag ccgcgaggcg ttgcgcgacc
    13261 gcgttgcccg ccagcaccag cgcggtctgg taaacaaggc tcagtccggt cacgtgcagc
    13321 atcatcgccg ccagcgtggc cggcgacgaa tcggcgcgca ggaacagcgg gaaaaaagcg
    13381 acgaagaaca gcatgacctt ggggttggtc aggctgaccg cgaacgcctg gcggaaatac
    13441 acccaggcgc tgcggcgggg ctcggcgtcg ccgtgcgtgg cgggtgaacg cgcctgcagc
    13501 agttgcagcc cgatccagat caggtagccg gcgccgaacc actgcaatgc ggtgaacagc
    13561 atcggattcg cgttcatcac cgccgccagt ccgagcacgg ccgcgagcat gtacgcgaag
    13621 tcgccgacca gcgtgccggc gaccgcgccc atgcccgcgc cgatgccgtt gcgcgcggtg
    13681 gcgttcagga tcgcgagcgt gcccggtccc ggaacggcct ggaacacgag cacggccgcg
    13741 atgaagctgg gatagtgctg aatgtcgaac atcttcgtcg tcccttgctc gaagccgatc
    13801 cggattctgc gctacgcagt tctccgaacg gctgcgcgcc agccgcaatg ttaggacggc
    13861 gtggcgagct tcaggccgac gatgccggcg acgatcagcg ccacgctgcc gagccgcagc
    13921 gcgctcgccg gctcaccgaa cagcacgatg cccaggatca cggtgcccac cgcaccgacg
    13981 ccgacccaga cgctgtacgc cgtgccgacc ggcagcgtgc gcatcgcgat tcccagcagc
    14041 cagaggctgg cgatcatcgc tgccacggtc cataccgtcg gccacggtcg ggtgaagccc
    14101 tgcgtgtact tcagtccgat cgcccatccg acctcgaaca gacccgcaag cacgagaatg
    14161 acccaggaca tcgtcgcccg ctccgattga aatcgctcgt agaatccacg cgttgcagaa
    14221 cccgacggca agcgagtgct gcggaagttg ttggccccct catggcgccg gccgggtacg
    14281 acatccggac ggtacctgat cggctcggct gctcggatgt cacccccatc agtgcacggc
    14341 gccgcgtgca aaatgcgggc gcgcgacctt ccgagcccgc cggttgcgtc gtgaacctga
    14401 cgtgacgacg tttcgcaatc ccgagcacga gatccaccac ttccgcaccc gcgtggtggc
    14461 ggccacgctg ttcgtgctcg tctgcttcgg cgcgctgctg gcgcgcttcg tctggctgca
    14521 ggtgatcaag cacgcggagc tgtacgagca ggccgagatc aaccgcatcg cggtggtgcc
    14581 ggtgcaggcc aaccgcgggc tgatccgcga ccgcaacggc gcgatcatcg cgcgcaactt
    14641 ttccgcctac acgctcgaga tcaacccgcg caaggtcgag aacgtcaacg cggtgatcga
    14701 cgagctggcc ggcgtgatcg aggtccagcc gcgcgaccgc cgccgcttca agaaactgca
    14761 ggaggagctg aagggcgccg agtcgatccc gatccgcacg cgcctgaccg acgacgaggt
    14821 cgcgcgcttt gccgcgcagc gcttccgctt ccccggcgtc gagctgcgcg cgcggctgtt
    14881 ccgccactat ccgctcggcg aggtcggcgc tcacatgatc ggctacatcg gacgcatctc
    14941 gccggccgac aaggagcgca tcgcgacgtt cgaggacacc gacaactacg tcggcgccga
    15001 acacatcggc aagaccggca tcgagctgtc gtaccagcgc gaactgcacg gcgccaccgg
    15061 ttcggagcgg gtcgagatca ccgccggcgg acgcgcggtg cgcacgctgg accgcagccc
    15121 gtcgacgccg ggcaacaacc tgaacctgtc gatcgacatc aagctgcagc agatcgtcga
    15181 gcaggcgttc ggcgatcgcc gcggcgcgct gatcgcgatc gagcccgaga cgggcgacgt
    15241 gcttgcgttc gtgtccaagc cgagcttcga cccgaatctg ttcgtcgacg gcatcgatcc
    15301 gaagaactgg gacgaactga acaacgatcc cgacaagcca ctcctgaacc gcgcgctgcg
    15361 cggcacctac ccgatcggct cgacctacaa gccgttcctc gcgctggccg ccctggagac
    15421 cggcaagcgc aggcccgagc aggtgatcta cgacccgggc tacttcgtgt tcggcaaccg
    15481 gcgcttccgc gacagcaaca aggtgccgct gggaccggtc gacatgcaca agtcgatcgt
    15541 caagtcgagc gacatctatt actacatggt cgccaacgat ctgggcgtgg acacgatcca
    15601 cgacttcatg aagccgttcg gcttcggcca gatcacgggc atcgatctcg aaggcgagca
    15661 gaccggcgtc ctgccgtcgt cggcctggaa ggagaagcgc ttcaagcaga agtggttccc
    15721 gggcgagacg atctcgatcg gcatcggtca gggctacaac gcgttcacgc tgctgcaact
    15781 ggcgcacgcg accgccacgc tcgccaacga cggcgtggtg atgaagccgc acctggtcaa
    15841 gagcgtcgag gacgtgaaga ccggcgaggt gcggctgacg gtgccgaagg agagctaccg
    15901 gattccgctg aaaccggagc acatccgctt cgtgcgcgag gcgatggtcg acgtgaacat
    15961 cagcggcacc gggcggcagg cgttcgccgg cgtgccgtac aaggtcgccg gcaagaccgg
    16021 caccgcccag gtgatcggca tcaagcagaa cgagaagtac aacgccaacc gcatcgacga
    16081 gcggcaccgc gaccattcgc tgttcatcgc gttcgcgccc gccggcccgg gcgagaagcc
    16141 gaagatcgcg ctggcgctgc tggtggaaaa cggcggcttc ggttcgatgg ccgcggcgcc
    16201 gatcgcgcgc caggcgatcg attactacct gctcggcaag gtgcccaacg caccggcgcc
    16261 gctgaagccg gaaggcgacg gcgagccatc gggagagttg gtcgcacaag tgaacggagg
    16321 cgcccgtgag cagtgagcgg cgtcggtgcg ttcgtcaacc tttgccacag gcgcagcgcg
    16381 ttacgtgccc cgccccagcg ccattgaaac cagagggcag cgccgaaccc gcgggcgaac
    16441 gggtcgcgca ggtcaacggg ggcacgcatg aacagtgaca cgctccgcgc gcgcatctgg
    16501 ctgtgggtgc gcgagcgcct gtccgtgctc gacccggcgc tgctcgcgat catcggcggt
    16561 ctgatgctgc tggggctgat cacgctgtac tcggcggcgc tcgactttcc gtggcgcttc
    16621 accgaccaga tgcgcaattt cgccatcgcc gccggcgtga tgttccttgt cgccagcatt
    16681 ccgccgagcg tgctgatgaa ggcggcgctg ccgatctacg tgatcggcct cgtgctgctg
    16741 gtcgccgtcg accgcttcgg cgtcatcgtc aagggcgcgc agcgctggct cgacctcggc
    16801 ttcacgcgca tccagccctc ggaggtgatg aagcttgcgc taccgatgat gctggcgtgg
    16861 tatttccaca agcgcgaagg cgcggtgcgc tcgctcgatt tcctgttggc ggcgctgctg
    16921 ctgatcttgc cggtcggcct gatcgccaag cagcccgatc tcggcacggc gatcctggtg
    16981 ctggcggcgg gcttcttcgt gatcttcttc gccggcctgc cgtggaagtg gctggcggcg
    17041 atgggggtgg cgggcgccgc gatgctgccg gtcgcgtgga ccttcatgca tggctaccag
    17101 cgcgagcgga tcatgacgct gatcgatccg tcgcaggacc cgctcggcaa gggcttccac
    17161 acgctgcagg cgatgatcgc gatcggcagc ggcgggctca cgggcaaggg ctggatgcag
    17221 ggcacgcaga cgcacctgga gttcattccc gagcgcacca gcgacttcat cttcgcggtc
    17281 tactcggagg agttcgggct gatcggcaac gtggtgctgg tcgtgctgtt cttcgcgctc
    17341 gtcacgcgcg cgctgctgat cgccgccaat gccgcgacca tctacgcgcg cctgatggcg
    17401 gcggcgatcg cgctgatcat cttcacctac ggcttcgtca acatgggcat ggtgagcggc
    17461 atcctgcctg tcgtgggcgt gccgctgccg ttcgtgagct acggcggcac ggcgctcgtc
    17521 acgctcggca tggcgtgcgg catgctgatg agcatcgcgc gcaatcgcaa gctggtgcag
    17581 tcgtgaattc ggtcggcggt cggcggtcgg cagtcgtcgg cctgctggcg gctgcactgc
    17641 tggccggctg cgcgacggcg ccgaagccgc cgcagcgcgc gggcgggtac taccagaacg
    17701 acgggccgcc ggcgcaggtg ccggcggacc tcgccaacgt gccggacgcg gtgccgcgcg
    17761 tggagccgct gcacccgtat gccaaccggc cctacgtcgc gctcggcaag agctatacgc
    17821 cgctcgccgc ggacgtgccg ttccggcaac gcggactggc ctcgtggtac gggcggcaat
    17881 tccacggcaa ccgcacgtcc agcggcgaga tctacgacat gttcgcgatg accgcggcgc
    17941 acccgacgct gccgatcccg agctatgcgc gcgtgaccag tgtcaagagc ggcgccagcg
    18001 tgatcgtgcg cgtcaacgat cgcgggccgt tcaaggacga ccgcatcatc gacctgtcgt
    18061 acgccgccgc gacgaaactc ggcttcgcca a
//
LOCUS       DBNHBY010000185         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1025476, whole genome shotgun sequence.
ACCESSION   DBNHBY010000185 DBNHBY010000000
VERSION     DBNHBY010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1025476"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="AC16HB_04575"
     CDS             <1..1511
                     /locus_tag="AC16HB_04575"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5606098.1"
                     /translation="VRCFAERPRDLNAMFAQAVALNGAGEALIDGDRRLIWSALDDEV
                     ARVAAGLARRGVERGERMALLFGNRAEFVTATLAAARLGAIVVPLSIRAQAPELAYML
                     EHSGARLVVHEAELANRLPAGPTRVAVGNCPGSEPFSSLLGDARTEAAPDGKEEDTAA
                     ILYTSGTTGRPKGALLTHLGIVHSTLHYASTMRLTDRDRSIAAVPLSHVTGVIAHIAT
                     WLRCAGALILLREFKASAFLELAARERITHTVLVPAMYNLCLLTPELDRFDLSAWRIG
                     GYGGAPMPPATIERCAQKLPRLELMNLYGATETTSPATIMPARFTATRPDSVGLTVAC
                     GHIVVVDDLGREVAPGATGEIWIGGPMVVKGYWNDPQATAREFTAGYWHSGDLGSKDA
                     EGFVRVFDRKKDLINRGGYKIYSAEVESVLIAHPAVVEAAVVAKPCPVLGERVHAFVC
                     LRADTDVADLRAHCAGQLSDYKVPETFTLTREPLPRNANGKLLKRELRVQLA"
     gene            1523..>1728
                     /locus_tag="AC16HB_04580"
     CDS             1523..>1728
                     /locus_tag="AC16HB_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5606099.1"
                     /translation="MSDAQAPLIFTRDGAIARLVFNRPAVLNALDRATAQAFAQACHT
                     LAADRSIRAVIMSGAGRAFMAGGDL"
BASE COUNT          261 a          605 c          605 g          257 t
ORIGIN      
        1 tcgtgcgctg cttcgccgag cggccgcgcg atctcaacgc gatgttcgcg caggccgtcg
       61 ccctcaatgg cgccggcgag gcgctgatcg atggcgaccg cagactgatt tggagcgcgc
      121 tcgacgacga ggtcgcacgt gtggccgccg ggctcgcgcg gcgtggcgtc gaacgcggcg
      181 agcgcatggc gctgctgttc ggcaaccgcg ccgaattcgt gaccgcaacg ctggctgcag
      241 cgcgactcgg cgcgatcgtg gtgccgctgt cgatccgcgc gcaggcgccg gagctcgcct
      301 acatgctcga gcacagcggc gcgcgcctcg tcgtgcacga ggcggaactc gcgaaccgcc
      361 ttccggccgg gccgacgcga gttgccgtcg gcaactgccc gggctccgag ccgttttcgt
      421 cactgctcgg cgacgcgcgg accgaggctg cccccgacgg caaggaagaa gacaccgcgg
      481 cgatcctgta cacgtccggc acgaccggcc ggccgaaggg cgcgctgctg acgcacctgg
      541 gcatcgtgca ctcaaccctg cactatgcgt cgacgatgcg cctgaccgac cgcgaccgct
      601 cgattgccgc ggtgccgctg tcgcacgtga ccggcgtgat tgcgcatatc gccacctggc
      661 tgcgctgcgc cggcgcgctg atcctgctgc gcgagttcaa ggcctcggcc tttctcgaac
      721 tggcggcgcg cgagcggatc acgcacacgg tgctggtgcc ggcgatgtac aacctgtgcc
      781 tgctcacgcc ggaactcgat cgcttcgacc tgtccgcatg gcgcatcggc ggctatggcg
      841 gcgcaccgat gccgccggcg acgatcgagc gttgcgcgca gaagctgccg cggctcgagt
      901 tgatgaacct gtacggcgcg accgagacca cgtcgcccgc gacgatcatg ccggcgcgct
      961 tcaccgcgac ccgccccgac agcgtcggac tgaccgtggc gtgcggccac atcgtggtgg
     1021 tggacgatct cggacgcgag gtcgcgccgg gcgcaacggg tgaaatctgg atcggcggtc
     1081 cgatggtggt caagggctac tggaacgacc cgcaggccac cgcgcgcgag ttcacggccg
     1141 gctactggca ttcgggcgac ctgggatcga aggacgccga gggcttcgtg cgcgtgttcg
     1201 accgcaaaaa ggacctgatc aaccgcggcg gttacaagat ctacagcgcc gaggtcgaga
     1261 gcgtgctgat cgctcatccc gcggtggtcg aggccgcagt ggtggcgaag ccatgtcccg
     1321 tgctcggcga gcgcgtgcac gcgttcgtct gcctgcgcgc ggacacggac gtcgccgacc
     1381 tgcgcgcgca ctgcgctgga cagctctccg actacaaggt gcccgagacc ttcacgctga
     1441 cgcgcgagcc gctgccgcgc aacgccaacg gcaagctgct gaagcgcgaa ctgcgagtgc
     1501 aactggcgta gtcttggccg ccatgagcga cgcccaagcc ccgctgatct tcacccgcga
     1561 cggcgccatc gcgcggctgg tcttcaaccg cccggcggtg ctcaacgcac tcgaccgcgc
     1621 aactgcgcag gcgttcgcgc aggcgtgtca cacgctcgcg gccgacaggt ccatacgcgc
     1681 ggtgatcatg agcggggcgg ggcgcgcgtt catggccggc ggcgatct
//
LOCUS       DBNHBY010000186         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_958150, whole genome shotgun sequence.
ACCESSION   DBNHBY010000186 DBNHBY010000000
VERSION     DBNHBY010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_958150"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16HB_04585"
     CDS             complement(<1..296)
                     /locus_tag="AC16HB_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5606100.1"
                     /translation="MRVGLIVVTYERPDALQRVLQSVGRQTRAPDELIVADDGSGPAV
                     ARIVADHARSAATPVRHVRQEHAGFRAGRVRNVGVAATDCDYIVLIDGDMVLHPR"
     gene            complement(296..>1797)
                     /locus_tag="AC16HB_04590"
     CDS             complement(296..>1797)
                     /locus_tag="AC16HB_04590"
                     /inference="COORDINATES: protein motif:HMM:NF044969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR5606101.1"
                     /translation="VGDRTNDAVRVDGRDLRCKVVGEGGNLGFSQLGRIEFALRGGRM
                     NTDFVDNSGGVDCSDHEVNIKILLKTVQERTRLKPAERNRLLAAMTDDVAQLVLRDNY
                     LQSQAISLLQSTAPERIGEHAHFIRTLELDGLLDRALEYLPSAEDIDDRRRTGRGLVR
                     PELAMVLSYAKIALNAQLIQSDVPEDPYLSQELDRYFPERLATRYGKLLGEHRLRREI
                     ITTATTNSIVNRMGPTFVSRTQQDTGADAAAVARAYAIAREAFEVRATWSAIEALDNK
                     VEAAVQYGMVHDTVGLLRQTTYWLLQRYRTSLGIEQQVARLRPGLRELAQAPLQWLQG
                     SDRAAFESRSAELARAGVPAEITIRVAACPALHCGPDIVELAAARKLTVEAAARAYFA
                     VGEHFGLDWLRAQVEGLDTQGHWHAVARGSLREALFEAHRALAQDVLTSTREADPAKA
                     LQRWLQRHEAAAAHARAVVSDIRAQGTGIDFASLSVALQAVRRLAVATA"
BASE COUNT          240 a          638 c          639 g          280 t
ORIGIN      
        1 cgtggatgca gcaccatgtc gccgtcgatc agcacgatgt agtcgcagtc ggtggcggcg
       61 acgcccacgt tacgcactcg gccggcgcgg aagccggcgt gttcctgccg cacgtgacgc
      121 accggcgtcg ctgcgctgcg cgcgtgatcc gcgacgatgc gtgcgaccgc cgggccggag
      181 ccgtcgtcgg ccacgatcag ttcgtccggt gcacgcgtct gccgcccgac gctctgcagc
      241 acacgctgca gggcgtcggg tcgctcgtag gtgacgacga tcaggccgac gcgcatcagg
      301 ccgtcgcgac cgcaaggcgg cgcaccgcct gcagcgcgac cgagagcgag gcgaagtcga
      361 tgcccgtgcc ctgcgcgcgg atgtcgctga ccaccgcccg cgcgtgcgcc gccgcggcct
      421 cgtgccgctg cagccagcgc tgcagtgcct tggcgggatc cgcttcacgc gtcgacgtca
      481 gcacgtcctg cgcgagtgcg cggtgcgcct cgaacaacgc ctcgcgcagg ctgccgcgcg
      541 cgacggcgtg ccagtggccc tgcgtgtcga gtccctcgac ctgtgcgcgc agccagtcga
      601 gcccgaaatg ctcgccgacc gcgaaatacg cgcgtgccgc ggcttcgacc gtcagcttgc
      661 gggccgccgc gagctcgacg atgtccggtc cgcagtgcag cgccgggcag gccgcgacgc
      721 gaatcgtgat ctcggcaggc acgcccgccc gcgcgagttc ggccgagcgc gattcgaagg
      781 cggcgcgatc cgagccctgc agccactgca gcggggcctg ggccagttcg cgcaggcccg
      841 gacgcagacg cgcgacctgc tgctcgatgc cgagcgacgt gcggtagcgc tgcaacagcc
      901 agtaggtcgt ctggcgcagc aggcccaccg tgtcgtgcac catgccgtac tgcacggccg
      961 cctcgacctt gttgtcgagc gcctcgatcg cgctccacgt ggcgcggacc tcgaacgcct
     1021 cgcgcgcaat ggcatacgcc cgcgcgacgg cggccgcgtc ggcaccggtg tcctgctgcg
     1081 tgcggctcac gaacgtcgga cccatgcgat tgacgatgct gttggtcgtc gcggtcgtga
     1141 tgatctcgcg gcgcagccgg tgctcgccca gcagcttgcc gtagcgcgtt gccagccgct
     1201 ccgggaagta gcggtccagt tcctgcgaga ggtaagggtc ctccggcacg tcggactgga
     1261 tcagctgtgc gttcaaggcg atcttggcgt agctcagcac cattgccagt tcgggacgca
     1321 ccaggccccg gccggtgcgg cgccggtcgt cgatgtcctc cgcgctgggc aggtactcga
     1381 gcgcgcggtc gagcaggccg tcgagctcca gcgtgcggat gaagtgcgcg tgctcgccga
     1441 tgcgttccgg cgccgtggac tgcagcaggc tgatcgcctg gctctgcagg tagttgtcgc
     1501 gcagcaccag ttgcgcgacg tcgtccgtca tcgcggcgag caggcggttg cgctccgccg
     1561 gcttgaggcg cgtgcgctcc tgcaccgtct tgagcaggat cttgatgttg acctcgtgat
     1621 ccgagcagtc gacgccgccg gagttgtcga cgaagtccgt gttcatgcgc ccgccgcgca
     1681 gcgcgaactc gatgcggccg agctgcgaga agccgaggtt gccgccttca ccgaccacct
     1741 tgcagcgcag gtcgcgcccg tcgacgcgca cggcgtcgtt ggtgcggtcg ccgactg
//
LOCUS       DBNHBY010000187         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_822437, whole genome shotgun sequence.
ACCESSION   DBNHBY010000187 DBNHBY010000000
VERSION     DBNHBY010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_822437"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2244
                     /gene="tssM"
                     /locus_tag="AC16HB_04595"
     CDS             <1..>2244
                     /gene="tssM"
                     /locus_tag="AC16HB_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HIR5606102.1"
                     /translation="NSMFKALLSRPVLVTAGLIAASALIWYLGPLLTLGGLRPLAPDW
                     SRWLAITVFSGLAASTVAARGVRSARTNRRLLDGLVAATPAVAAPGAHEVATIARRFE
                     EAVALLKRSRLGGRRSALDALAGRPYLYQLPWYVIIGAPGAGKTTALANSGLEFPLAS
                     KFGKRGIRGVGGTRNCDWWFTSDAVLIDTAGRFTTQDSDPKADHAAWSAFLDLLRKYR
                     PRRPVNGVLLTVSVSDLLGASAEDRREHARRLRERVDELTARFGAGIPIYVLITKLDL
                     LAGFMEFFADFDKDERAQVWGVTFPYGGNDAQADPLARLSNELVALEKRLDERLLERL
                     QGESDRERRAAIYAFPQQWRVLRETLNDFLLASFGGAQPSARPLVRGVYFTSATQEGT
                     PMDRALGSLARSLGLANRIVAPARPTGKTFFVTRLLREVVFAEAGLSGTNLRWQRGRR
                     LLEWSAIAATAVTVAFALAFAWRGYRDNQQYLAAVQSRVAEVERSVATARAAPATDLG
                     ALLPVLDALPTIGAPDAAQTGTAALRRGFDLGLDQSAKLAAAGQDAYRRLLRDALLPR
                     IAARLQDRLRANATERVDTIYEALKAYLMLFSGQHFDRAGLGTSLRADWDLAMPGAEG
                     GDTGARLRRHFDQLIASGEVGAPVLADRQLIDRTRALVARVPLAQRVYTRLKSAGLEA
                     AASGLPDFTLEAVAGPAVRQVFARASGRPTSQGVPALYTRAAFQQLARQSDAIVRQLA
                     AESSWVL"
BASE COUNT          299 a          783 c          814 g          348 t
ORIGIN      
        1 tgaacagcat gttcaaggcg ctgctcagcc ggccagtgct cgtgactgcg gggctgatcg
       61 cggcgagcgc actgatctgg tacctcgggc cgctgctcac gctcggcgga ctgcggccgc
      121 tcgccccgga ctggtcacgc tggctcgcga tcaccgtctt cagcggtctg gcggcatcga
      181 ccgtggcggc gcgcggcgta cgcagtgcgc gcaccaaccg ccggttgctc gacggcctgg
      241 tagccgccac cccggcggtg gccgcgccgg gtgcgcacga agtcgcaacg atcgcgcgac
      301 gcttcgaaga ggccgtggcg ctgctcaagc gctcgcgtct gggcggacgc cgctccgcgc
      361 tcgatgcgct cgccggtcga ccgtatctgt atcagctccc ctggtacgtc atcatcggcg
      421 cgccgggcgc cggcaagacg acggcactcg caaactccgg cctcgaattc ccgctcgcgt
      481 cgaaattcgg caagcgcggc atccgcggcg tcggcggcac gcgcaactgc gactggtggt
      541 tcaccagcga tgccgtgctg atcgacaccg ccggccgttt cacgacgcag gacagcgacc
      601 ccaaggccga tcatgcggcc tggtccgcgt ttctcgatct gctgcgcaag tatcggccgc
      661 gccggccggt caatggcgtg ctgctgacgg tcagtgtcag cgacctgctc ggcgcgagcg
      721 ccgaagaccg gcgtgaacac gcgcggcgcc tgcgcgagcg cgtcgacgaa ctgaccgcgc
      781 gcttcggcgc gggaattccg atctatgtgc tgatcacgaa gctcgacctg ctggcgggct
      841 tcatggagtt cttcgctgac ttcgacaagg acgagcgcgc gcaggtgtgg ggcgtgacct
      901 ttccgtatgg cggcaacgac gcgcaagccg atccgcttgc gcgtttgtcg aacgagctgg
      961 tggcgctcga gaagcgcctc gacgagcgcc tgctcgagcg gctgcagggc gagagcgacc
     1021 gcgagcgccg tgcggcgatc tacgccttcc cacagcagtg gcgggtgctg cgcgagacgc
     1081 tgaacgactt cctgctcgcc agcttcggcg gtgcgcagcc gtctgcgcgg ccactggtgc
     1141 gcggcgtcta tttcaccagc gccacccagg agggcacgcc gatggatcgc gcgctcggca
     1201 gcctcgcgcg ctcgctcgga ctggccaacc gcatcgtggc gccggcgcgc cctaccggca
     1261 aaacgttctt cgtcacacgg ctcctgcgcg aagtggtgtt tgccgaagcg ggcttgtccg
     1321 gcaccaacct gcgctggcag cgcggacgcc ggctgctcga gtggagcgcg atcgcggcga
     1381 ctgcggtcac ggtggcgttc gcgctggcct ttgcgtggcg cggctatcgc gacaaccagc
     1441 agtatctggc cgcggtgcag tcgcgcgtgg ccgaggtgga gcgcagcgtc gcaaccgcgc
     1501 gggcggcgcc ggcgaccgat ctcggtgcgc tgctgccggt gctcgatgcg ctgccgacga
     1561 tcggcgcgcc ggacgccgcg cagaccggta ctgcggccct gcgccgcggg ttcgatctcg
     1621 gtctcgacca gagcgcgaag cttgccgccg ccggtcagga cgcgtatcgc cgcctgctgc
     1681 gggacgcgct gctgccgcgc atcgcggcgc ggttgcagga ccggctgcgt gcgaacgcga
     1741 cggagcgcgt cgacacgatc tacgaagcgc tgaaggccta cctgatgctt ttcagcggcc
     1801 aacacttcga tcgcgccggg ctgggtacgt cgctgcgtgc cgactgggac ctcgcgatgc
     1861 cgggcgccga gggtggcgat accggcgcgc ggctgcgccg gcacttcgat cagctgatcg
     1921 cgagcggcga ggtcggcgcg cccgtgctcg ccgatcggca actgatcgat cgcacgcgcg
     1981 cgctggtcgc gcgcgttccg ctcgcgcagc gcgtgtacac gcggctgaag agcgccggtc
     2041 tggaggcggc cgcgagtggc ctgcccgact tcacgctcga ggccgtcgcc ggcccggcgg
     2101 tgcgtcaggt gttcgcgcgc gcgagcggcc gtcccacctc gcaaggggtg ccggcgctgt
     2161 acacgcgcgc ggcctttcag cagctggcac ggcagagcga cgcgatcgtg cgccagcttg
     2221 ccgcggagtc gagctgggtg ctgg
//
LOCUS       DBNHBY010000188         6847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1025561, whole genome shotgun sequence.
ACCESSION   DBNHBY010000188 DBNHBY010000000
VERSION     DBNHBY010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1025561"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..820
                     /locus_tag="AC16HB_04600"
     CDS             134..820
                     /locus_tag="AC16HB_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5606103.1"
                     /translation="MTTLPRFDLYALIHKALRAAMTDTLTAVGRMDPLDDAETAATLA
                     RVRALLALARTHLEKENEFLHPALEARRPGSSAAVAHDHAHHEESLAALEGLIGRMES
                     APAGARAGDAGRLYRALALFVAENFEHMHLEETELNAALWAAYSDDELHAIHDALVAS
                     VEPQKMAEVVRWMVPNIAPFERAVVLGDMQRKAPAAVFSGVLDLVRPSLSARDWDKLM
                     NALTPLPVAA"
     gene            complement(998..2359)
                     /locus_tag="AC16HB_04605"
     CDS             complement(998..2359)
                     /locus_tag="AC16HB_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5606104.1"
                     /translation="MSPALSNPLEAIRTCLEGLIPSLVASCSADGTPNVTYVSQVHYV
                     DREHVALTYQFFNKTRENILANPHVTAFVGDTTVGRRYLLRLRYLRTEESGPTFESMK
                     ARLAGIASHSGMTGVFRLRGADIYRVLAVEPVDGPLLPPQPRRYQPMSAVRQIAQRIA
                     AAGDLRALLDEALLSLEQHLDIHYAKVLMLEAGKSRPARLYTVASRGYEISGVGSEIP
                     LGQGVIGVAAEQRVPIRIMHFTAEYTYSRAVREQWIACGAADQLETEIPLPGLAEPHS
                     QLAVPIVLGSRVVGALYVESEEAARFDYDDEDALLAVATHLGATIAALQTAADASEES
                     PGVPTSALPAAGEPIVIRHFAANDSVFVDDEYLIKGVAGAILWKLAREHAAGRRSEFT
                     NRELRLDPSLRLPDISDNLEARLILLARRLEERVPSMRIVKTGRGRFRLDVARPLRLA
                     AND"
     gene            complement(2356..2838)
                     /locus_tag="AC16HB_04610"
     CDS             complement(2356..2838)
                     /locus_tag="AC16HB_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606105.1"
                     /translation="MSYTLDEQHAEFIARPHASLAVASRDANNVPSLFKAAACRVSRD
                     RQRVTLYVDQNLATSVLRDLRAGHPIAAVFTEPATHRSIQLKGERADIGPATPADREY
                     ARERIESVVTHLVALKYPEAGVRCYFHSVPEQLVAVSFAPIAAFEQTPGPSAGARLNK
                     "
     gene            complement(2981..3229)
                     /locus_tag="AC16HB_04615"
     CDS             complement(2981..3229)
                     /locus_tag="AC16HB_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606106.1"
                     /translation="MSPNLKPGSTGRFRPTVLSDRTVPHLDREFVELQDKPEVSAIGS
                     LVGSMGGACQRANKPYLDWPREQSVGMHVMVSHAAATT"
     gene            3299..4186
                     /locus_tag="AC16HB_04620"
     CDS             3299..4186
                     /locus_tag="AC16HB_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606107.1"
                     /translation="MDRKPLDALAFGLMVVLTMLWGMQQVAIKLALPGVSPVAQAAIR
                     STVATVLLLAWARARAIPLFDRDRTLVPGLLAGLLFAGEFFLIYIGLNHTSASRMVVF
                     VYLAPILTALGLAWLVPGERLTGVQWAGVFLAFAGLALAFADGFATQARSTVIGDACG
                     VVAAALWAATTVLIRATRLAPARAEKTLLYQLAVSALLLALASPAIGEPGVMRIDAAV
                     IASLVFQSVVVAFASYLAWFWLLTKYLAARLAVFSFLAPMFGVAFGVLLLGESLTLRF
                     GLAALGVGAGIALVNLRRR"
     gene            4549..5736
                     /locus_tag="AC16HB_04625"
     CDS             4549..5736
                     /locus_tag="AC16HB_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606108.1"
                     /translation="MTNALEIQRRSALRGIAAAAMLCALPHAALAADPFKVGLILPMT
                     GPFASTGKQIEAAVRLYMAKNGDTVAGRKIQVIVKDDTGLAPETTKRIAQELVVQEKV
                     GVLAGFGLTPLALATAPVATEAKVPMIVMGAATAMIPTRSPFIVRPNFTLPQVTGPIA
                     EWAAKNKIKRVFTLVTEYGPGLDAEKTFHAVFKAAGGEIVEAVRTPLRNPDYAPFLQR
                     AKDAKPEALFVFVPSGEGLAVMKQFDERGLKQAGIRLIGTGDVTDDDLLESMGPASVG
                     VITSFHYSAAHDSPENKAYVDAFIKANNGMRPNFHSIGGYDGMHLIYEALKKTGGTGT
                     GEQLVEAMKGMKWISPRGPMSIDPATRQPVQNVYLREVKMVNGKPWNIEFDKVENVKD
                     PGN"
     gene            5774..6640
                     /locus_tag="AC16HB_04630"
     CDS             5774..6640
                     /locus_tag="AC16HB_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606109.1"
                     /translation="MFGNLIGVLFDGVAYGSLLFVISIGLSVTMGLMNFVNLAHGAFA
                     MLGGYACVMLMTRAGVPFLATLPVAFLVSAVAGIVLERSLYQRLYRATHLDQVMFSIG
                     IVFMAVAATTYFFGPSQQPVRLPEFLRGQVQVLGLGLGAYRLFLIGVVALVTIALHLL
                     LTRTRFGAQVRAAVDNAVASAGLGINVNRVFGITFALGSGLAGLGGGLGIDVLGLDPT
                     FPLKYMVYFLLVVAVGGAGTIKGGLVAAMILGICDVAGKYYVPQIGSFIIYLLMVALM
                     IAFPTGLYGRRA"
     gene            6637..>6847
                     /locus_tag="AC16HB_04635"
     CDS             6637..>6847
                     /locus_tag="AC16HB_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606110.1"
                     /translation="MSDASDAAGRASTALGAIVPAVPRLPTDRWHSLEVAFWLAAAAA
                     FFAFPDHRVLGSQILITGLFAVALDL"
BASE COUNT         1029 a         2280 c         2341 g         1197 t
ORIGIN      
        1 attgaaacgc agattttcac acacgaaatc gtcttacgaa gttcttaggg aatccttagc
       61 aaacaattag cggcgcggtc gcgtcctcgt ttctacgctg tgggctcgac actgaaccga
      121 ccaggagact gccatgacga ccttgccccg cttcgacctc tacgcgctca tccacaaggc
      181 actgcgcgcc gcaatgaccg atacgttgac tgccgtcggc cggatggatc cgctcgacga
      241 cgccgagacc gccgccacgc tggcgcgcgt gcgcgcgctg ctggcgctgg cccgtacgca
      301 cctggagaag gagaacgaat tcctgcatcc cgcgctcgag gcgcgccgac cgggatcgag
      361 cgcggcggtc gcgcacgatc acgcacacca cgaggagtcg ctcgccgcgc tcgaaggtct
      421 gattggccgc atggaatcgg cgccggcggg cgcgcgcgcc ggcgacgcgg gccggctgta
      481 tcgcgcgctg gcgcttttcg tcgccgagaa cttcgaacac atgcacctcg aggaaaccga
      541 actgaacgcc gcgctgtggg cggcctacag cgacgacgaa ctgcacgcga tccacgatgc
      601 gctggtggcc tcggtcgagc cgcagaagat ggccgaagtc gtgcgctgga tggtgccgaa
      661 catcgcgccg ttcgagcgcg cggtcgtgct cggcgacatg cagcgcaagg cgcccgccgc
      721 ggtcttcagc ggcgtcctcg acctggtgcg cccgagcctg tcggcgcgcg actgggacaa
      781 gctgatgaat gcactgacgc cgctgccggt cgcggcctga cctgagtctc acggagaaga
      841 agatgttgaa gaggttgcac tggtccgaat tcgccttgtg gggcgtggcg ttgattgcgg
      901 tcgcgctcgc ggcaggcccc gcgttcgcgt agggcgctac gcccagcgcg gcgctcgagg
      961 ggttgttcgt gatgttcccg gcgctgcggc aatagcgtca gtcgtttgcg gccagccgca
     1021 gcggacgcgc gacgtcgagc ctgaagcgcc cgcgcccggt cttgacgatg cgcatcgacg
     1081 gcacgcgctc ctccaatcgg cgcgccagca ggatcaggcg cgcctcgagg ttatcgctga
     1141 tgtcaggcag ccgcagcgac ggatcgagcc gtagctcgcg gttggtgaac tcggagcggc
     1201 gcccggccgc atgctcgcgc gccagcttcc acaggatcgc gccggccacg cccttgatca
     1261 ggtattcgtc gtcgacgaac acgctgtcgt tggcggcgaa gtggcggatc acgatcggct
     1321 cgccggctgc gggaagcgca ctcgtcggca cgccgggcga ttcctcgctg gcgtcggcgg
     1381 cggtctgcag cgcggcgatc gtcgcgccga gatgggtggc gaccgcgagc agcgcgtcct
     1441 cgtcgtcgta atcgaagcgc gccgcctcct cgctctccac gtatagcgcg ccgacgaccc
     1501 ggctgccgag cacgatcggc accgcgagct ggctgtgcgg ttcggccagc cccggcagcg
     1561 ggatttcggt ctccagctga tcggccgcgc cgcacgcgat ccactgttcg cgcacggcgc
     1621 ggctgtatgt gtactcggcg gtgaagtgca tgatgcggat cggcacgcgc tgctcggcgg
     1681 ccacgccgat cacgccctgc ccgagcggaa tttccgagcc gacgcccgag atctcgtagc
     1741 cgcgcgacgc aaccgtgtac aggcgggccg ggcgcgactt gcccgcctcc agcatcagca
     1801 ccttcgcgta gtggatgtcc agatgctgtt cgagcgaaag caaggcttcg tcgagcaagg
     1861 cgcgcaggtc acccgcggcg gcgatgcgct gcgcgatctg gcgtaccgcc gacatcggct
     1921 gatagcgccg cggctgcggc ggcagcagcg gtccgtcgac cggctcgacg gccagcacgc
     1981 gatagatatc ggcgccgcgc agcctgaata cgccggtcat gcccgagtgc gaggcgatgc
     2041 cggccagcct cgccttcatg ctctcgaacg tcggcccact ctcctcggtg cgcaggtacc
     2101 gcaaacgcag caggtagcgc cggccgaccg tggtgtcgcc gacgaacgcc gtcacgtgcg
     2161 gattggcgag gatgttctcg cgcgttttgt tgaagaactg gtaagtgagt gccacatgct
     2221 cgcgatcgac gtaatgcacc tgcgacacat aggtcacgtt cggcgtgccg tcggcgctgc
     2281 agctggcgac cagcgacggg atcagtccct ccaggcaggt gcggatcgcc tcgagcgggt
     2341 tcgacagcgc cgggctcatt tgttcaacct cgcgccggca ctcgggccgg gcgtctgctc
     2401 gaacgcggcg atcggcgcga acgacaccgc gaccagctgc tccggcacgg aatggaagta
     2461 gcagcgcacc ccggcttcgg ggtacttgag cgccacgagg tgcgtgacca cgctttcgat
     2521 ccgctcgcgc gcatattcgc ggtcggccgg ggtcgccggc ccgatgtcgg cgcgctcgcc
     2581 cttcagctgg atcgaccggt gcgtcgcggg ctcggtgaac accgcggcga tcgggtggcc
     2641 cgcgcgcaga tcgcgcagaa cgctcgtggc caggttctga tcgacataca acgtgacgcg
     2701 ctgccggtcc cggctcacgc ggcaggccgc ggccttgaac agcgacggca cgttgttcgc
     2761 gtcgcgcgac gcgaccgcga gcgacgcgtg cggccgcgca atgaactcgg cgtgttgttc
     2821 gtcgagggtg tagctcatgg ccagcctcgc gcgtgttgcg tgatgcggag cggggcatcc
     2881 taactccgct caccccgaac cggctacggc ctcctgggtg ccaggcgtcc gttccacttc
     2941 ttgccgtcga tgatcgcgcg ttcgggcgtg ccggccggga tcaggtcgtg gctgccgcgt
     3001 gcgagaccat cacgtgcatc ccgacgctct gttcccgcgg ccagtccaga taaggcttgt
     3061 tcgcgcgctg gcacgcgcct cccatcgacc cgacgagaga gccgatggcg gacacctccg
     3121 gcttgtcctg caattcgacg aactcgcgat ccaggtgcgg cacggtcctg tcgctcaaca
     3181 ccgtggggcg gaaccggccg gtgctgcccg gcttcaggtt cgggctcatg cacttgattc
     3241 cgtcaactcg cgtcgcgacg attctatgga atgatgccgg ccgcactggc ctgccctgat
     3301 ggatcgcaaa ccgctcgacg ccctcgcatt cggcctgatg gtcgtgctca cgatgctgtg
     3361 gggcatgcag caggtcgcga tcaagcttgc cctgcccggt gtatcgccgg tcgcgcaggc
     3421 ggcgatccgc tccacggtcg ccacggtact gctgctcgcc tgggcgcggg cgcgcgcgat
     3481 tccgctcttc gatcgcgacc gcacgctcgt gcctggcctc ctggccgggc ttctgttcgc
     3541 cggtgagttc tttctgatct acatcggact gaaccacacc tcggcgtcgc gcatggtggt
     3601 gttcgtctac ctggcgccga tcctgaccgc gcttggcttg gcatggctcg tgcccggcga
     3661 gcgcttgacc ggcgtgcagt gggccggcgt gttcctcgct tttgcgggac tggcgctcgc
     3721 gttcgccgac ggcttcgcca cgcaggcgcg cagcacggtg atcggcgatg cgtgtggcgt
     3781 ggtcgccgcg gcgctgtggg ccgcgaccac tgtgctcatc cgcgccacgc gactcgcgcc
     3841 cgcgcgcgcc gagaagacgc tgctgtacca actcgcggtg tcggcgctgc tgcttgcgct
     3901 tgcgtccccc gcgatcggcg agccgggcgt gatgcgcatc gacgcggcgg tgatcgcgag
     3961 tctcgtgttc cagagcgtcg tggtcgcgtt cgccagctat ctcgcctggt tctggctgct
     4021 gaccaagtac ctcgcggcgc gacttgccgt gttctcgttc ctcgcgccga tgttcggcgt
     4081 ggcgtttggc gtgctgctcc ttggcgaatc gctgacgctc cgctttggcc tcgccgcgct
     4141 gggggtcggc gcggggattg cccttgtgaa cctgcgccgc cggtgagcgg ctatccgtac
     4201 cgttccgacg gccgcagtcg tgtcgctcga actcggtggc ttgcggacct catatcgaaa
     4261 tggtctagca gctagtgcag cgatcacatt gctgaataga ccggcgtcac taagaatcgc
     4321 gcatgacagg cactgccggg tctcgcgcgt tcgggaggcg actggcccag cgagggtggc
     4381 gtcgacagcg tcgcgggcgg ccctggtttg ccggagactg caggatgccg cgcggcgtct
     4441 ggcaccaatt gagacgccaa ggcatgcgca tgcagacgag aggggagtgc gtgccgttcc
     4501 tggcagtttc gtaaccccgg gttcctgacc cacgaggagg agatgcacat gaccaatgcg
     4561 ctggaaatcc aacgccgcag cgcgctgcgc ggcatcgctg ccgccgccat gctttgcgcg
     4621 ctgcctcacg cggcgctggc ggccgatccg ttcaaggtcg gcctgatcct gccgatgacc
     4681 ggaccattcg cgtccacggg caagcagatc gaggccgcgg tgcggctgta catggccaag
     4741 aacggcgata cggtcgcggg ccgcaagatc caggtgatcg tgaaggacga caccgggctt
     4801 gcgccggaga ccaccaaacg cattgcgcag gaactcgtcg tgcaggagaa ggtcggcgtg
     4861 ctcgccggct tcggcctgac gccgctggca ttggccaccg caccggtggc caccgaggcg
     4921 aaggtgccga tgatcgtgat gggggccgcc acggcgatga tcccgacccg ttcgcctttc
     4981 atcgtgcgtc cgaacttcac gctgccgcag gtgaccggac cgatcgcgga atgggcggcg
     5041 aagaacaaga tcaagcgcgt gttcacgctg gtcaccgagt acggcccggg gctcgacgcg
     5101 gagaagacct tccacgcggt gttcaaggcg gccggcggcg agatcgtcga ggcggtgcgc
     5161 acgccgttgc gcaatccgga ctacgcgccg ttcctgcagc gggcgaagga cgcgaagccc
     5221 gaggctctgt tcgtcttcgt gccgtccggg gaggggctcg cggtgatgaa gcagttcgac
     5281 gagcgcggcc tcaagcaggc aggcatccgg ctgatcggca ccggtgacgt caccgacgac
     5341 gacctgctcg agagcatggg tcccgcgtcg gtcggcgtga tcacctcgtt tcactactcg
     5401 gcggcgcacg actcgcccga gaacaaggcc tacgtcgatg cattcataaa ggcgaacaac
     5461 ggcatgcggc cgaacttcca ttcgatcggc ggctacgacg gaatgcacct gatctacgag
     5521 gctctcaaga agaccggcgg caccggcacc ggagagcagc tcgtcgaggc gatgaaaggc
     5581 atgaagtgga tcagcccgcg cgggccgatg tcgatcgacc cggcgacccg ccagcccgtg
     5641 cagaacgtct acctgcgcga agtgaagatg gtcaacggca agccgtggaa catcgaattc
     5701 gacaaggtag agaacgtcaa ggaccccggc aactgaacca cggcggttcg agcccgcggt
     5761 ttcggggcgc gcgatgttcg gcaacctgat cggcgtcctg ttcgacggcg tcgcctacgg
     5821 cagcctgctc ttcgtgatca gcatcggcct gtcggtgacg atgggtctga tgaacttcgt
     5881 gaatctcgcc catggcgcgt tcgcgatgct cggcggttac gcctgcgtga tgctgatgac
     5941 gcgcgcgggc gtgccgttcc tcgcgacgct gcccgtcgcc ttcctggtct cggccgttgc
     6001 gggcatcgtc ctcgaacgga gcctgtacca gcggctttat cgggcgacgc acctcgacca
     6061 ggtgatgttc tccatcggca tcgtcttcat ggcagtcgcg gcgacgacct atttcttcgg
     6121 gccgtcgcag cagccggtgc ggctgccgga attcctgcgc ggacaagtgc aggtgctcgg
     6181 cctcggcctc ggcgcgtacc ggctgttcct gatcggcgtc gtcgcgctgg tgacgatcgc
     6241 gctgcacctg ctgctgacgc gcacgcgctt cggcgcgcag gtccgcgccg cggtcgacaa
     6301 tgccgtcgcc tcggcgggcc tgggaatcaa cgtcaaccgc gtgttcggca tcacgtttgc
     6361 gctcggctcg ggactcgcgg gactgggcgg aggtctgggc atcgacgtgc tcgggcttga
     6421 tcccactttc ccgctcaagt acatggtgta tttcctgctc gtcgtcgcgg tcggcggagc
     6481 cggcacgatc aagggcgggc tcgtcgccgc gatgatcctc gggatctgcg acgtcgccgg
     6541 caagtactac gttccgcaga tcggctcgtt catcatctac ctcctgatgg tggcgctgat
     6601 gatcgccttc ccgacgggcc tgtacggcag gcgagcatga gcgatgccag cgatgccgcg
     6661 ggaagggcga gcaccgcgct tggagccatc gtgccggcgg tgccgcggct gccgaccgac
     6721 cgctggcact cgctcgaggt cgcgttctgg ctcgcggccg cggcggcatt cttcgcattt
     6781 ccggaccacc gtgttctggg cagccagatc ctgatcaccg gacttttcgc cgtcgcgctc
     6841 gacctga
//
LOCUS       DBNHBY010000189         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_484594, whole genome shotgun sequence.
ACCESSION   DBNHBY010000189 DBNHBY010000000
VERSION     DBNHBY010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_484594"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1052)
                     /gene="trxA"
                     /locus_tag="AC16HB_04640"
     CDS             complement(186..1052)
                     /gene="trxA"
                     /locus_tag="AC16HB_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5606111.1"
                     /translation="METTQQAFSHDVIEASHRNPVLVDFWAPWCGPCRALGPMLEKIE
                     TEFVGRLRVVKINTDENQEIAAQFNVRSIPYVVAFVDGRAIDSFVGVMPEGQLRSFVE
                     RLLPNPSELERRKAARLVAAGDIAGAAVALRAAVALSGDNESARLDLAALLLDHAEAM
                     GGSSAVEEAGQLLTAAPRATREDPVWRALSTRFDSMRRATELPSAESLRARIEAMPAD
                     LQARLDLARRHIARSEFESALEQLLAIVERDRNFGEDIGRKTMLSVFDLAADQPQLVA
                     AFRRRLSAALNR"
     gene            complement(1120..1791)
                     /locus_tag="AC16HB_04645"
     CDS             complement(1120..1791)
                     /locus_tag="AC16HB_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HIR5606112.1"
                     /translation="MSISLFSRSGSKSDRARKPDNFVHSVLSYTKLEVPTVEVFETAV
                     TFAPDTSPLSAHAELAGAFGQRAGEGHFLFRADSTIGGRYWVQSVKPWPQRPARAVSA
                     LEPKRTLIQVAPGLMYRFTLLVCAGRARLEGAEKEVEPFRTKEEVEHWFKGTAERYGM
                     RLLMADVTMTALRFEHRGSRIRIDHAAIEGALEVLQPDFFRARILRGFGHHRRAGLGM
                     IQLSA"
     gene            2107..2394
                     /gene="groES"
                     /locus_tag="AC16HB_04650"
     CDS             2107..2394
                     /gene="groES"
                     /locus_tag="AC16HB_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5606113.1"
                     /translation="MKIRPLHDRVIVKRLDQERKTASGIVIPDTAAEKPDQGEVVAVG
                     PGKRDDNGKLIAPDVKVGDRVLFGKYAGQTVKVEGEELLVMREEDLMGVIA"
     gene            2425..>2705
                     /locus_tag="AC16HB_04655"
     CDS             2425..>2705
                     /locus_tag="AC16HB_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR5606114.1"
                     /translation="MAAKQVFFHDDARHRMVRGVNVLANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEVELKDKFENMGAQMVKEVASRTSDDAGDGTTTATV"
BASE COUNT          494 a          861 c          896 g          454 t
ORIGIN      
        1 tttccggcaa caccgccgtc gacgcgccgt cgggttgcgc gaatgttgcg ccggccgcca
       61 gccataccat gagcgcggca atggctcgac gcgaacgcgc cgacggcagg agtacagcgg
      121 cgaacattgc gaccggactg aatgcggcta cgacggcgca cagcttagcc gccgcgcgtc
      181 gcgattcagc gattgagcgc ggcggacagg cggcgccgga aggcggcaac cagttgtggc
      241 tgatctgccg cgagatcgaa cacggacagc atcgtcttgc ggccgatgtc ttcgccgaag
      301 ttgcggtcgc gttcgacgat cgcgagcagt tgttcgagcg cggactcgaa ctcgctgcgc
      361 gcgatgtgcc gacgggccag gtccagtcgc gcctgcagat ccgccggcat cgcctcgatg
      421 cgcgcgcgca gcgattcggc ggacggcagt tcggtcgcgc ggcgcatgct gtcgaatcgg
      481 gtcgagagcg cgcgccagac gggatcctcg cgggtggccc tgggcgctgc ggtgaggagt
      541 tggccggcct cttcgaccgc agaggagccc cccatcgcct cggcatggtc cagcagcaat
      601 gccgccaggt cgagtcgcgc cgactcgttg tcccccgaga gtgccacggc ggcgcgcagt
      661 gcgaccgcgg cgccggcgat atcccccgcc gcgaccagtc gcgcggcttt gcggcgctcc
      721 aattcactcg gattcgggag cagccgctcc acgaaggaac gcaactggcc ttcgggcatc
      781 acgccgacga aggagtcgat cgcccggccg tcgacgaacg cgacgacgta cgggatgctg
      841 cgcacgttga actgcgcggc aatttcctgg ttctcgtccg tgttgatctt gacgacacgc
      901 agtcggccga cgaattcggt ctcgatcttc tcgagcatcg gcccgagcgc gcgacatgga
      961 ccgcaccatg gtgcccagaa atcgaccagc accgggttgc ggtgcgacgc ctcgatgacg
     1021 tcatgcgaga aagcctgctg ggtggtttcg atcatcggtt gcctcgatcc ttcagatcgg
     1081 gtcgagggcg caaagctcaa gcccgccgat cgtcgcccat catgccgaca gctgaatcat
     1141 gcccagcccg gcgcgccgat gatggccgaa gccgcggaga atgcgggctc ggaagaaatc
     1201 gggttgcagc acctcgagtg ccccttcgat cgccgcatga tcgatccgga tgcggctgcc
     1261 acgatgttcg aagcgcagcg cggtcattgt cacgtcggcc atcaacagac gcatgccgta
     1321 gcgctcggcc gtccccttga accagtgctc gacctcctcc ttggtgcgga acggttcgac
     1381 ttccttttcg gcgccctcaa gccgggcgcg gcccgcgcac acgagcagcg tgaaccggta
     1441 catcagaccc ggcgcgacct ggatcagggt ccgcttcggt tccagtgcgc tgaccgcgcg
     1501 cgccggccgc tgcggccagg gcttgaccga ctgcacccag tagcggccgc cgatcgtgct
     1561 gtcggcgcgg aaaagaaaat ggccttcgcc ggcgcgttgg ccgaacgccc cggcgagttc
     1621 ggcgtgtgcc gacagcggac tcgtatccgg cgcgaaagtg accgcggttt cgaacacctc
     1681 gaccgtcggc acctcgagct tcgtgtaact caggaccgag tggacgaagt tatcgggctt
     1741 gcgcgcacgg tccgacttcg atccgctgcg ggaaaacagg ctgatgctca tggttcgatg
     1801 cgtgtccaac cgcaatgcga accgggcccg gcagaaaacc cgcgggcccg tcgcgcgcga
     1861 gtgcttagta gcgtatcgcc gatcgcggcg aaatcccttc ggatcattag gctattttgc
     1921 tgaagccgac cgtcgcgctt gcgcgtgtcc gcgatcgtcg ggggcttgaa cgggcggggc
     1981 gatgccctta ttattagcac tcgaaagcgg cgagtgctaa gagtccggcg cagccgcctt
     2041 tccatttctg gaggtcggcc tccacccttt ccgtaattcg aaaaagtcct ttaaccagga
     2101 gagtttatga aaattcgtcc attgcacgat cgcgtgatcg tcaagcggct ggaccaggag
     2161 cgcaagaccg cttccggcat cgtgattccg gacactgctg cggagaagcc cgatcagggc
     2221 gaagtggtgg cagtcggccc gggcaagcgc gatgacaacg gcaagctgat tgcccccgat
     2281 gtgaaggttg gcgaccgggt gctgttcggc aagtacgcag gacagaccgt caaggtcgag
     2341 ggcgaggaac tcctcgtgat gcgcgaggaa gacctcatgg gcgtgatcgc ctgatgacga
     2401 cgattcagaa cggagaaatc aaacatggca gctaaacagg tcttcttcca cgacgacgcg
     2461 cgtcatcgca tggtccgcgg cgtcaacgtc ctggccaacg cggtcaaggt gacgctcggc
     2521 cccaaaggtc gcaacgtcgt tctggagcgc tccttcggcg ccccgacggt gacaaaggac
     2581 ggcgtgtcgg tcgccaagga ggtcgaactg aaggacaagt tcgagaacat gggtgcgcag
     2641 atggtcaagg aagtcgcttc caggacttcc gacgacgcgg gtgacggcac cacgaccgcg
     2701 accgt
//
LOCUS       DBNHBY010000190         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1296418, whole genome shotgun sequence.
ACCESSION   DBNHBY010000190 DBNHBY010000000
VERSION     DBNHBY010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1296418"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..405
                     /locus_tag="AC16HB_04660"
     CDS             226..405
                     /locus_tag="AC16HB_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606115.1"
                     /translation="MKTTRDPSFHYTPSYMTDVKKTFERVRRRQLKELEKSAPAPVTP
                     VNIASLVRKKAGGRS"
     gene            complement(662..>1654)
                     /locus_tag="AC16HB_04665"
     CDS             complement(662..>1654)
                     /locus_tag="AC16HB_04665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR5606116.1"
                     /translation="NLTVITDTLVTRLRLQGKRAVGVEFVRGSEAQFAEARLETVMAA
                     GSIGTTQILQLSGIGPAALLKQHGIDVAHELPGVGENLQDHLQLRMGFKVRNVLTLNK
                     LANSWIGKARMALEYALFRTGPLTMAPSQLGGFTRSSGQHATPNIEYHVQPLTLDKFG
                     DPLHEFPAFTASVCNLRPTSRGHVRIKSADPRAAPAIQPNYLSTPEDHQVAIDSLRLT
                     RRIVQAPALARYTPEEFLPGPQVQGDAELVRAAGDIGTTVFHPIGICRMGRPGDRMAV
                     VDPRLRLLGLGGLRVVDASIMPTITSGNTNSPVLMIAERGAEMIRQDRRSARSG"
BASE COUNT          269 a          513 c          569 g          303 t
ORIGIN      
        1 attcttgagg ctgcgcggac gacggcaaga ttgttgcttg ccgtgttcgg atatcgccgg
       61 cgcgcgtctg atctgcggtt ctcgctgcgc agtgatttca gggaagcgca agtctttctt
      121 gcggcgcagc gttgcgttgc gttgtgaaag gtgatcgtca gacgcgtgcg caccacgtct
      181 gcgcgtgcac ctgcgtcgag ggtgcgtctc gccgcgggag gccatatgaa aacgacccgt
      241 gatccatcgt ttcactacac gccaagctac atgacggacg tgaagaagac cttcgaaaga
      301 gtccggcgcc gtcagctcaa ggagttggag aaaagcgcgc cggcgccggt gacgccggtc
      361 aacattgcgt cgctggtcag gaagaaggcc ggcggacgca gctgagtctc aggaacccag
      421 aagtcgcagg ttcggcccga cgggcacggg ttgatcggtg gtcattgcag cgcagaacgc
      481 ccagcagcgg cgttccttca atgcgtgcgt gacggatctc agggaaatga acttcacgag
      541 cgcctcccaa gtcatctgtc ccttgggttg caggcagcgc cggtcgggtt caaggtcgct
      601 tcgatcgatg cctcgcatcc gtccggccaa tccgtgctga ccgggcggat gccgcagttc
      661 gctatccgct gcgcgctgat cttcggtcct gccggatcat ctccgccccg cgctcggcga
      721 tcatcagcac gggcgagttg gtattgcccg atgtgatcgt cggcatgatc gacgcgtcga
      781 caacgcgcag gccgcccagg ccgagcaggc gcaggcgcgg atcgacgacg gccatgcggt
      841 cgccgggccg gcccattcta cagataccga tcggatggaa gaccgtggtg ccgatgtcgc
      901 cggcggcgcg cacgagttcg gcatcgcctt gcacctgcgg tcccggcagg aactcttccg
      961 gcgtgtaacg cgcgagcgcg ggcgcctgca cgatacgtcg cgtgagccga agcgaatcga
     1021 tcgcgacctg gtggtcctcg ggcgtcgaca ggtagttggg ctggatcgcg ggcgcggcgc
     1081 gcggatcggc gctcttgatg cgcacgtggc cgcgcgacgt cgggcgcagg ttgcacacgc
     1141 tggcggtgaa cgccggaaac tcgtgcagcg gatcgccgaa cttgtccagc gtcagcggct
     1201 gcacgtgata ttcgatgttc ggcgttgcgt gctggcccga tgagcgcgtg aagccgccga
     1261 gctgcgacgg tgccatcgtc aacgggccgg tgcggaacag cgcgtattcg agcgccattc
     1321 tcgccttgcc gatccagctg ttggcgagct tgttcagcgt cagcacgttg cgcaccttga
     1381 agcccatacg cagttgcagg tggtcctgca ggttctcgcc gacgccgggc agttcgtgcg
     1441 cgacgtcgat gccatgctgc ttcagcagcg cggcggggcc gatgccggac aactgcagaa
     1501 tctgcgtggt cccgatcgag ccggcggcca tcacggtctc caggcgtgcc tcggcaaact
     1561 gcgcctcgct gccgcgcacg aactcgacgc cgaccgcgcg cttgccttgc agtcgcagcc
     1621 gcgtgacgag cgtgtcggtg atgacggtga gatt
//
LOCUS       DBNHBY010000191         4749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1093934, whole genome shotgun sequence.
ACCESSION   DBNHBY010000191 DBNHBY010000000
VERSION     DBNHBY010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1093934"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16HB_04670"
     CDS             complement(<1..190)
                     /locus_tag="AC16HB_04670"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5606117.1"
                     /translation="MKALIFAAGRGERMRPLSDVTPKPLLVAGGRRLIEWQIDALVRA
                     GVRDLVINTAHLATSIETT"
     gene            complement(198..1217)
                     /locus_tag="AC16HB_04675"
     CDS             complement(198..1217)
                     /locus_tag="AC16HB_04675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR5606118.1"
                     /translation="MNDSTPDPRLRQLQRWLTSLPSSLRLQPESLRPASADASFRRYF
                     RLDAANTTLVAMDAPPDKEDSRPFVHIAALLNAAGLNAPRIVAQDLAQGFLLLTDLGT
                     TTYLTALNADNADRLFADAIDALIRWQLASRANELPPYDEALLRRELLLFPDWYVARH
                     LQRTLSPTQQQALDGAFRAILDVCLAQPRVYVHRDYMPRNLMLSDPNPGVLDFQDAVY
                     GPVTYDVVSLFRDAFVSWDEERVLDWTIRYWEKARRAGLPVGTDFADFWREFEWMGLQ
                     RHLKVLGIFARINYRDGKPHYLADTPRFVMYVRSVTQRYVALAPLARLIDELEGVERR
                     AGYTF"
     gene            1335..3551
                     /locus_tag="AC16HB_04680"
     CDS             1335..3551
                     /locus_tag="AC16HB_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR5606119.1"
                     /translation="MFRILAAALLVTANALAQTTDAPVVLRIERALGAGRVADAPPGA
                     TYARAQRVSGEIDERIVLEGEAEVRRGGTVLRGDRIEYSVPDDQVTVTGQARVFREGA
                     VVTGPSLKLKVDAQTGSMPDASFYYAQQNGRGRCALIEFLGEDKTRLNDTTYTTCAPG
                     DDSWWVKADRLDLDRAEELAVARGTSIHFMGVPIFASPYFQFPLGDKRRSGFLTPSFG
                     INSRLGAEGTLPYYWDIAPNMDATITPRLMQRRGVLLGNEFRYLEPTFRGTAQYEILP
                     NDRVTGTSRWLGSLRHEYVNPLGVVGGVNYNRVSDDRYFADFSRSIVTASQSVLPQEA
                     FIGFNRPYWNTALRVTKNQTLQDPLAPVVKPYERVPQVAFNARNTDWRGFDVSLAVEA
                     TRFDHPSLETGSRVLVNPSVSYPWLAPGYFVVPKVQWHATSYALDAAVHPGDTHPTRA
                     LPLTSVDAGLVFEREARWFGAPSVQTLEPRLFYASVPFRDQSKLPNFDSALADFNFAQ
                     LFTENIFVGSDRIGEANQVTTALVGRVLDPDSGAERVRALVGQRFYFAPQRVTLPGGT
                     ERTGNASDILFALSGAPARYWYVDAALQHSTELSRLVRTSVALRWQPRPASVLSVAYR
                     YKLDELEQVDLAGQWPLAPRWYGVGRINYSLRDSRWVELLGGVEYKADCWVARVVMHR
                     FVTTAQTATTAIFFQLQLNGLASIGSSPIEQLRRNIPGYQVVNPPPREPGRFDIYE"
     gene            3555..>4749
                     /locus_tag="AC16HB_04685"
     CDS             3555..>4749
                     /locus_tag="AC16HB_04685"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5606120.1"
                     /translation="MKLKSAARSAAFCLLAALSGGVLAQAAPPPDAPAAAPARAPAKL
                     LDRIVAVVNDEAVTLAELQARVRITEQQLKRQNIPSPPADVLRRQVLERMIVDRAQLQ
                     LARESGVRVDDATVNAAIARIADQNNATLPVFRERLEREGIAFARFREDVRDDIVMSR
                     LRDREVDSRIQVSEGEIDNFLAAQAGVEAGAIEYNIAQILLRVPDNATTDRIEETRKR
                     AQELLEQLRSGADFARAAASFSNAAEALQGGELGWRTAERLPSLFLDAVKDLKPGELG
                     TIVRSPGGFHILKLIGRRNAVEGKLSTAAVEQTHVRHVLLRVSDLTPESEVKRRLSDL
                     RDRVLKGGQDFGQLARLHSVDGSSTRGGDLGWLYPGDTVPEFERAMNQLKPGEISEPV
                     QTPFGW"
BASE COUNT          770 a         1610 c         1593 g          776 t
ORIGIN      
        1 gcgtggtctc gatgctcgtg gccaggtgcg cggtgttgat caccagatcg cgcacgccag
       61 cgcgcaccag cgcgtcgatc tgccattcga tcagtcgtct cccgcccgcc accagcagcg
      121 gctttggcgt cacgtccgac agcggccgca tccgctcgcc gcggccggcg gcgaagatca
      181 gggctttcat gccaggctca gaacgtgtac cccgcccgcc gctcgacgcc ttccagttcg
      241 tcgatcaggc gtgccagcgg cgcaagcgcg acgtagcgct gcgtgacgct gcggacgtac
      301 atgacaaagc gcggcgtgtc ggcgagatag tgcggcttgc cgtcgcggta attgatacgt
      361 gcgaagatgc cgagcacttt caagtggcgc tgcagtccca tccattcgaa ttcgcgccag
      421 aagtccgcga agtcggtgcc gaccggcaac ccggcgcgac gcgccttctc ccagtagcgg
      481 atggtccagt cgagcacgcg ctcctcgtcc caagacacga acgcatcgcg gaacagcgat
      541 acgacgtcat aagtaaccgg tccgtacacg gcatcctgga agtcgagcac gccggggttc
      601 ggatcgctca gcatcagatt gcgcggcatg tagtcgcggt gcacgtacac gcgcggctgc
      661 gcgaggcaga cgtcgaggat cgcacggaac gcgccatcga gcgcctgctg ctgtgtcggg
      721 gacagcgtgc gctgcagatg gcgcgcgaca taccagtcag gaaagagcag cagttcccgc
      781 cgcagcagcg cctcgtcgta aggcggcagt tcgttcgcgc ggcttgccag ctgccatcgg
      841 atcagtgcgt cgatcgcatc ggcgaagagt cggtcggcat tgtccgcgtt cagcgcagtc
      901 aggtacgtcg tcgtgcccag atcggtgagc agaagaaagc cctgcgcgag atcctgtgcg
      961 acgatgcgcg gtgcgttcag ccccgccgcg ttcagcagcg ctgcgatgtg cacgaacggt
     1021 cggctgtcct ccttgtcggg tggcgcgtcc atcgccacca gcgtggtgtt cgccgcgtcg
     1081 agccggaaat agcggcgaaa gctcgcatcg gccgacgccg ggcgcagcga ttccggctgc
     1141 agccgcaagc tcgacggcag cgacgtgagc cagcgctgca gttgccgcag gcgcggatcg
     1201 ggcgtcgaat cgttcatcgt gtgcggttgc gtgctggaag gtgggcgcga aggccgttcc
     1261 tatcgaaggc cgttcctata atagccacgc gatccgaacc acaatcaatg ccacgctcaa
     1321 cgtcgcgcgc gcccgtgttc cgaatcctcg ccgcggcgct gctggtgact gcgaatgcgc
     1381 tggcgcagac gaccgatgcg ccggtcgtgc tcaggatcga gcgcgcgctc ggcgccggcc
     1441 gcgtcgcgga cgcgccgccc ggcgccacct acgcgcgcgc acagcgcgtc tccggcgaga
     1501 tcgatgaacg catcgtgctc gaaggcgagg ccgaagtgcg ccgcggcggc accgtgttgc
     1561 gcggcgaccg catcgaatac tcggtgcccg acgatcaggt gaccgttacc ggacaggcgc
     1621 gcgtgtttcg cgaaggtgcg gtcgtgaccg gcccgagcct gaagctgaag gtcgacgcac
     1681 agaccggcag catgccggac gcgagcttct actacgcgca gcagaacggg cgcggccgct
     1741 gcgcgctgat cgaatttctc ggcgaagaca agacgcgcct gaacgacacg acctacacga
     1801 cctgcgcgcc cggcgacgat tcgtggtggg tgaaggcaga ccggctcgac ctcgaccgcg
     1861 cggaggaact cgccgtcgcg cgcggcacgt cgatccactt catgggcgtg ccgatcttcg
     1921 cctcgcctta cttccagttc ccgcttggcg acaagcggcg cagcggtttc ctgacaccga
     1981 gcttcggcat caactcgcgc ctgggcgccg agggcacgct gccgtactac tgggatatcg
     2041 cgcccaacat ggacgccacg atcaccccgc gactgatgca gcgccgtggc gtgctgctgg
     2101 gcaacgagtt ccgttacctg gagccgacct tccgtggcac cgcgcagtac gagattctgc
     2161 cgaacgaccg cgtcaccggc acctcgcgct ggttgggttc gctgcggcac gagtacgtca
     2221 atccgctcgg cgtggtgggc ggcgtcaact acaaccgcgt gtcggacgat cgctacttcg
     2281 cggatttttc gcgcagcatc gtgaccgcgt cgcagtcggt gctgccgcag gaggcattca
     2341 tcggtttcaa tcggccgtac tggaacaccg cgctgcgtgt gaccaagaac cagacgctgc
     2401 aggacccgct ggcaccggtg gtcaagccgt acgagcgcgt gccgcaggtc gcgttcaacg
     2461 cgcgcaacac cgactggcgc ggcttcgatg tatcgctcgc agtggaagcc acgcgcttcg
     2521 atcatccctc gctcgaaacg ggctcgcgcg tgctcgtcaa tccgagcgtg tcttacccgt
     2581 ggctggcccc cggctacttc gtggtgccga aggtgcagtg gcacgccacc tcctacgcgc
     2641 tcgacgctgc cgtgcatccg ggcgacacgc atccgacgcg cgcgctgccg ctcacatcgg
     2701 tcgacgccgg cctcgtgttc gagcgcgagg cgcgctggtt cggcgcgccg tcggtgcaga
     2761 cgctcgagcc gcgcctgttc tatgccagtg tcccgttccg cgaccagtcg aagctgccga
     2821 acttcgacag tgcgctcgcg gacttcaact tcgcgcagct cttcaccgag aacatcttcg
     2881 tcggcagcga ccgcatcggc gaggccaacc aggtgacgac ggcactggtc ggccgcgtgc
     2941 tcgaccccga ttccggtgcc gaacgcgtgc gcgcgctggt cggccagcgc ttttatttcg
     3001 cgccgcagcg cgtgacgctg cccggcggaa ccgagcgcac cggcaatgcg tccgacatcc
     3061 tgttcgcgct cagcggcgcg ccggcgcgct actggtacgt cgatgcggcg ctccagcatt
     3121 cgaccgagct cagccggctg gtgcgcacgt cagttgcgct gcgctggcaa ccgcggccgg
     3181 catccgtgct gagcgttgcc taccgctaca agctcgacga actggagcag gtcgacctcg
     3241 ccggacagtg gccactggcg ccgcgctggt acggcgtcgg ccgcatcaac tattcgctgc
     3301 gcgattcacg ctgggtcgag ctgctcggcg gcgtcgagta caaggccgac tgctgggttg
     3361 cgcgcgtcgt gatgcatcgc ttcgtcacga ccgcgcagac ggcgaccacc gcgatcttct
     3421 tccagctgca actgaatggt ctggccagca tcggctcgag tccgatcgag cagctgcggc
     3481 gtaatattcc aggctatcag gtcgtcaatc ctccgccccg cgagccgggg cgcttcgata
     3541 tctacgagta gccgatgaag ttgaagtccg cggcgcgcag cgccgctttc tgtcttttgg
     3601 ccgcgctctc cggtggcgta ctggcacaag ccgcgccgcc gccggacgcg cccgcggccg
     3661 ccccggcgcg cgcgccggcc aagctgctcg accgcatcgt cgccgtcgtc aacgacgagg
     3721 ccgtgacgct ggccgagctg caggcgcgcg tgcgcatcac cgagcagcag ttgaagcggc
     3781 agaacattcc gtccccgccg gccgatgtgc tgcgccggca ggtgctggag cggatgatcg
     3841 tcgaccgtgc gcagctgcag ctggcgcgcg agtccggcgt gcgcgtcgac gacgccaccg
     3901 tgaacgcggc gatcgcgcgc atcgcggacc agaacaacgc gaccctgccc gtgttccgcg
     3961 agcggctcga gcgcgaaggc atagcgttcg cgcgtttccg cgaggatgtg cgcgacgaca
     4021 tcgtcatgtc gcgcctgcgc gaccgcgagg tcgacagcag gatccaggtc tccgagggcg
     4081 aaatcgacaa ctttctcgcc gcgcaggcag gcgtcgaggc gggtgcgatc gaatacaaca
     4141 ttgcgcagat cctgctgcgc gtgccggaca atgccaccac cgatcgcatc gaagagacac
     4201 gcaagcgcgc gcaggagctg ctggagcagt tgcgcagcgg cgcggatttc gcacgtgcgg
     4261 cggcgagctt ctccaatgcc gcggaagcgt tgcagggcgg cgaacttggc tggcgcactg
     4321 ccgagcgact gccgtcgctg ttcctcgacg cggtgaagga cctgaagccc ggcgagctcg
     4381 ggaccatcgt ccgcagcccg ggcggctttc atatcctcaa gttgatcggc cggcgtaacg
     4441 cggtcgaagg caagctctct acggccgcgg tcgaacagac gcacgtgcgg cacgtcctgc
     4501 tgcgcgtgtc ggatctgacg cccgagtcgg aggtcaagcg ccggctgagc gacctgcgcg
     4561 atcgcgtact caagggcggt caggatttcg gccagctggc gcggctgcat tcggtcgacg
     4621 gaagctccac gcgtggcggc gatctcggct ggctctatcc gggcgatacc gtgcccgagt
     4681 tcgagcgcgc gatgaatcag ctcaagcccg gtgagatcag cgagccggtg cagactcctt
     4741 tcggctggc
//
LOCUS       DBNHBY010000192         5324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1567020, whole genome shotgun sequence.
ACCESSION   DBNHBY010000192 DBNHBY010000000
VERSION     DBNHBY010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1567020"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="ubiG"
                     /locus_tag="AC16HB_04690"
     CDS             complement(<1..599)
                     /gene="ubiG"
                     /locus_tag="AC16HB_04690"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR5606121.1"
                     /translation="MRTINARKERMSVNVDEAELRKFGELAHRWWDPQGTMRPLHEMN
                     PLRLDWIDRIASLADKRVLDVGCGGGILAESMAARGAQVTGIDLADKPLQVAELHALE
                     SGARVGYRKVSAEELAREMPATFDVVTCMEMLEHVPDPAQTVHACATLVRSGGAVFFS
                     TINRNPKSFLFAIVGAEYVLRLLPAGTHEYAKFIRPSELA"
     gene            757..1824
                     /locus_tag="AC16HB_04695"
     CDS             757..1824
                     /locus_tag="AC16HB_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606122.1"
                     /translation="MKMRKIAALLSTLFAFGAACAQAPAQPGTARFVAPGEAQPVAGA
                     RPIEVKAIDVLRGDGLFYFSGTRVVNLRAADKPQGERYPLTESTSDLGIGAFSANIDV
                     QNFVRDGERRPIGEPSFAVPDADGSMQLIYNAQHPLRLAVSMKAYDVSGLPIGDFLRT
                     AENYTRPEAAKTNGTRFPAGSVAYLASARFVDDVLMLPRRESFTGAANVKQMVTNFSK
                     DTPFCLAYEDRDGATPYALHFRGAGAKGKTELYPAKPGTMFCARASERPVAEGIWEER
                     TIGGTRAVVLSFGANVDPLDTGVTRAEREAALIAFVEPTRGAPGVRPGKLYRSGARIV
                     DFQYRFNGTAANAIRAALGVP"
     gene            complement(1896..2618)
                     /gene="ompA"
                     /locus_tag="AC16HB_04700"
     CDS             complement(1896..2618)
                     /gene="ompA"
                     /locus_tag="AC16HB_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpA"
                     /protein_id="HIR5606123.1"
                     /translation="MKKSVKLGYLIATAMVTASGAAFAQGKDVDGKPNTPAYVTDGRG
                     NVVFDPFGLCWRTPWWTTQLASQGGPKGAGCACDKAALPKEVCEPPPPPPPAPPPPPA
                     APPTPPPPPPPPPPPAPTSEKVTFAADALFDFNKSVLKPEATAKLDDLVSKLAGVNLE
                     VIVAVGHTDSIGSDSYNDKLSMRRAEAVKSYLVKKGIEANRIYTEGKGERQPVADNKT
                     AAGRAKNRRVEIEVVGTRTIRR"
     gene            complement(2738..4069)
                     /locus_tag="AC16HB_04705"
     CDS             complement(2738..4069)
                     /locus_tag="AC16HB_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR5606124.1"
                     /translation="MTPVDTLIECRWVVPIRPRGALENHAIAIDQGRIVALLPIGEAR
                     RRHQPRLRVELPHHVVTPGLVNAHTHAAMALLRGVGDDLPLMRWLTERIWPLEKALVS
                     EEFVYDGTRLAALEMLRAGVTCCSDMYFFPDACARALRDVGMRGVVGIIAIEFPSAYA
                     SDAEDYLRKGVATRDALRDDPLISFTLAPHAPYTVSDATFARIAMLAEELDLPVHMHV
                     HETQHEIDESIAQHGVRPLARLDRMGLVTERLIAVHAVHLDPVEIALLADRGASVAHC
                     PASNLKLASGIAQVALLLEAGVNVAIGTDGAASNNRLDLLGETRLAALLAKSAARDAA
                     AMPAQVALECATLGGARALGIERTVGSIEAGKMADLAAFDLSGPEENPCFEPVSHLVY
                     STDRSAVSDVWVAGRHVVRARQVVTHAGDEVSSPILAAITAWQNRSWQYLK"
     gene            4266..>5324
                     /locus_tag="AC16HB_04710"
     CDS             4266..>5324
                     /locus_tag="AC16HB_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5606125.1"
                     /translation="MESFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMQEANNVWNRPYVKCARIVGDVMGKFHPHGDAAIYDALVRMAQEFSLRYTL
                     IDGQGNFGSIDGDNAAAMRYTECRLEKISALLLADIDKETVDFQPNYDGKELEPTVLP
                     ARVPNLLINGSAGIAVGMATNIPPHNLNEIVDGCLLLLNMPDASIEALLELIPAPDFP
                     TAGIIYGLDGVREGYRTGRGRVVMRARTHFEGVDEKPGDKGARPAIIVDELPYQVNKR
                     VLLERMAELVNEKKLEGISDIRDESDKSGMRVVIELKRGELPEVVLNNLYKQTQLQDT
                     FGMNFVALVDGAPRLLNLK"
BASE COUNT          887 a         1763 c         1776 g          898 t
ORIGIN      
        1 gcgagctcgc tggggcggat gaacttcgca tactcgtgcg tgcccgcggg caggagccgc
       61 agcacgtact cggcgccaac gatcgcgaac aggaatgatt tcggattgcg gttgatggtg
      121 gaaaagaaca cggcgccgcc cgaccgcacg agcgtggcgc acgcgtgcac ggtctgcgcc
      181 gggtcgggca cgtgttcgag catctccata caggtgacca cgtcgaacgt ggccggcatc
      241 tcgcgcgcga gctcctcggc cgaaaccttg cgatagccga cgcgcgcgcc gctttcaagg
      301 gcgtgcagct cggcgacctg cagcggcttg tccgccagat cgattccggt cacctgcgcg
      361 ccgcgcgccg ccatcgattc ggcgaggatg ccaccaccgc agccgacgtc gagtacgcgc
      421 ttgtcggcca gcgacgcgat gcggtcgatc cagtccagcc gcagcgggtt catctcgtgc
      481 agcggccgca tcgtgccctg gggatcccac cagcgatgcg cgagttcgcc aaacttgcgc
      541 agttccgctt cgtcgacgtt gacgctcatt cgttccttgc gcgcattgat cgtgcgcata
      601 ctagccaagt gctcatccct ttgccggagc tgcgctccga ctgtgcgcgc cgctccggac
      661 taaccggatc aggggacatc gcgcgccgtg cggacggcag cgcgcgcggg ccgttggctt
      721 agattgcgca tcaccgcatt tgtacggagc ccgacgatga agatgaggaa gattgccgcg
      781 ctgttgtcta ccctgttcgc gttcggcgcg gcctgcgcgc aggcgccggc gcaaccgggc
      841 accgcgcgct ttgtcgctcc gggggaagcg cagccggtcg cgggcgcgcg gccgatcgag
      901 gtcaaggcga tcgatgtgct gcgcggcgac ggcctgttct acttctcggg cacccgcgtg
      961 gtgaacctgc gcgccgccga caagccgcag ggagagcggt atccgctgac cgaatcgacc
     1021 agcgatctgg gaatcggtgc gttcagcgcc aacatcgacg tgcagaactt cgtgcgcgac
     1081 ggcgaacggc ggccgatcgg cgagccaagc ttcgcggtcc ccgacgccga cggcagcatg
     1141 caactgatct acaacgcgca gcacccgctg cggctggcgg tttcaatgaa ggcgtacgac
     1201 gtctccgggc tgccgatcgg ggatttcctg cgcacggcgg agaactacac gcggcccgag
     1261 gccgcgaaga cgaacggcac gcgctttccc gccggctcgg tcgcgtacct ggccagcgcg
     1321 cgtttcgtcg acgacgttct aatgctgcca cggcgcgaat cgttcaccgg cgcggccaac
     1381 gtcaagcaga tggtcacgaa cttctcgaag gacacgccgt tctgccttgc gtacgaggat
     1441 cgcgacggtg ccacgcccta tgcactgcac ttccgcggcg ccggtgccaa gggcaagacc
     1501 gagctttatc cggccaagcc cggcacgatg ttctgcgccc gtgcgtcgga gcgcccggtg
     1561 gccgaaggca tctgggaaga gcgcaccatc ggcggcacac gtgccgtggt gctgtcgttc
     1621 ggcgcaaacg tggatccgct cgacaccggt gtcacgcgtg cggagcgcga agcagcgctg
     1681 atcgcgttcg tcgagcccac ccggggcgca ccgggggttc gaccgggcaa gctgtaccgg
     1741 tccggcgcgc gcatcgtcga cttccagtat cgcttcaacg gcacggccgc caacgcgatc
     1801 cgcgctgcgc ttggcgtccc ttgacctgtt gacctgcttg aggacgaaaa aaaagccccg
     1861 cggttgcggg gcttttccgt tgcgcgcgac tcgccttagc gacggatcgt ccgcgttccg
     1921 acgacctcga tctccacgcg gcggttcttg gcgcggccag cggcggtctt gttgtcggcg
     1981 accggctgcc gctcgccctt gccttcagtg tagatgcggt tggcttcgat acccttcttg
     2041 acgaggtacg acttcaccgc ttcggcgcga cgcatcgaca acttgtcgtt gtacgaatcg
     2101 ctgccgatcg agtcggtatg accgactgcg acgatcacct cgaggttgac acccgccagc
     2161 ttcgacacca gatcgtcgag cttggcggtc gcttccggct tcagtacgga cttgttgaag
     2221 tcgaacagcg cgtcagccgc aaacgtcacc ttctcgctcg tcggcgccgg cggaggcggc
     2281 ggtgggggcg gcggaggcgg tgtcggcgga gcagccggcg gcgggggtgg agccggcggt
     2341 ggcggcggag gcggctcgca gacctccttc ggcagtgcgg ccttgtcgca ggcgcagccg
     2401 gcgcccttcg gcccgccctg cgaggcaagc tgcgttgtcc accacggtgt ccgccagcac
     2461 aggccaaacg gatcgaacac cacgttgccg cggccgtcgg tcacatacgc cggagtgttc
     2521 ggtttcccgt cgacgtcttt gccttgagca aaggcggcgc cggacgcggt caccatcgcg
     2581 gtggcgatga gataaccgag tttcacggat ttcttcatgg ttctcctcac tcgtgtacgc
     2641 agaattcctg caccggttcg tgcgcaattc aggcgttgga ataccgtcct ccgcccgacc
     2701 tgcgccgcaa acgttgttgc cggttgcggg ccgccggtca cttcaaatac tgccaactcc
     2761 tattctgcca cgccgtgata gcggccaaga tggggctcga aacctcgtct cctgcatgcg
     2821 tgacgacctg tcgcgcgcgc acaacatgac ggcccgcgac ccacacatca ctgacggcgg
     2881 agcgatcggt cgagtaaacg agatgcgata ccggctcgaa gcaggggttt tcctcgggtc
     2941 cgctcagatc gaaagcggcc agatcggcca tttttccggc ctcgatcgag ccgaccgtgc
     3001 gctcgatgcc cagcgcgcgc gcgccgccga gcgtcgcgca ctcgagcgcc acctgcgcgg
     3061 gcatggcagc tgcatcccgc gctgcgcttt tggcgagcag cgctgcgagc cttgtttcgc
     3121 cgagcagatc gagccggttg ttgctcgcgg cgccgtcggt gccgattgcc acgttgacac
     3181 cggcctccaa cagcaacgcg acctgcgcga taccgcttgc aagcttcagg ttcgacgccg
     3241 ggcaatgcgc gaccgacgca ccgcggtccg ccaacaacgc gatctccacc gggtccagat
     3301 gcacggcgtg caccgcaatc agccgctcgg tgaccagccc cattcggtca aggcgggcga
     3361 ggggacgcac gccgtgctgc gcaatcgatt cgtcgatctc gtgctgcgtc tcgtgcacgt
     3421 gcatatgcac cggcaggtcg agttcctcgg ccagcatcgc gatgcgcgcg aaagtggcat
     3481 cggacaccgt gtacggtgcg tgcggcgcca gcgtgaaact gatcagagga tcgtcgcgca
     3541 gcgcgtcgcg ggtggccacg cccttgcgca gataatcctc ggcgtcgctt gcatacgcgc
     3601 tcggaaactc gatcgcgatg atgccgacca cgccgcgcat gcccacgtcg cgcagcgcgc
     3661 gcgcgcaggc gtcgggaaag aaatacatgt ccgagcagca ggtcactccg gcgcgcagca
     3721 tttccagcgc cgccagccgc gtgccgtcgt agacgaattc ctccgacacc agcgccttct
     3781 cgagcggcca gatgcgctcg gtcagccagc gcatcagcgg caagtcgtcc cccacgccgc
     3841 gcagcagcgc catcgcggcg tgggtatgtg cgttgacgag gcccggcgtg acgacgtggt
     3901 gcggcagttc gactcgcagg cgtggttgat gccggcgacg tgcctcgccg atcggcagca
     3961 gcgcgacgat gcggccctgg tcgatcgcga tcgcgtggtt ttccaacgca ccgcgcggcc
     4021 gtatcggcac gacccagcgg cattcgatca aggtatcgac gggcgtcatc gtgcacaccg
     4081 tggcggccgg attggagtgg cggcggatcg gcgcgcagtg tgccacggcg cccgggaaaa
     4141 gcggtgtgcg ctgatagact tgccgcttcg aactcacgtt gaatatgcgg tccggcggtc
     4201 ccctccgtcg cccgctttcg aaccgcgccg caacgcctga ggggcgcggt ccgcctcccc
     4261 cgccgatgga atcattcgcc aaggaaacgc tgccggtctc gctcgaagag gagatgcggc
     4321 ggtcctatct cgattacgca atgagcgtga tcgtcggccg cgccctgccc gatgtgcggg
     4381 acggcctgaa gccggtgcat cggcgcgtcc tgtacgcgat gcaggaggcg aacaacgtct
     4441 ggaaccggcc ctacgtcaag tgcgcgcgca tcgtcggcga cgtgatgggc aagttccatc
     4501 cgcacggcga cgccgccatt tacgacgcgc tggtgcgcat ggcacaggaa ttctcgctgc
     4561 gctacacgct gatcgacggc cagggcaact tcggctcgat cgatggcgac aatgcggcgg
     4621 cgatgcgcta caccgagtgc cgcctggaga agatttccgc gctgctgctg gccgacatcg
     4681 acaaggagac ggtcgacttc cagcccaact acgacggcaa ggaactcgag ccaacggtgc
     4741 tgccggcgcg ggtgccgaac ctgctgatca atggctcggc cggtatcgcg gtcgggatgg
     4801 cgaccaatat cccgccgcac aatctgaacg agatcgtcga cggctgcctg ctgctgctga
     4861 acatgccgga cgcgtcgatc gaagcgctgc ttgaattgat cccggcaccg gactttccga
     4921 cggcggggat catctacggg ctcgatggcg tgcgcgaagg ctatcgcacc ggccgtgggc
     4981 gcgtcgtgat gcgcgcgcgg acgcatttcg agggggtgga cgaaaagccc ggcgacaagg
     5041 gcgcgcgtcc cgcgatcatc gtcgacgaac tgccgtacca ggtcaacaag cgcgtgctgc
     5101 tcgaacgcat ggcggagttg gtcaacgaga agaagctcga gggcatttcc gacattcgtg
     5161 acgagtccga caagagcggc atgcgggtgg tgatcgagct gaaacgcggc gagctgcccg
     5221 aggtggtgct gaacaacctg tacaagcaga ctcagctgca ggacacgttc ggcatgaatt
     5281 tcgtggcgct ggtcgacggc gcgccgcggc tgctgaacct gaag
//
LOCUS       DBNHBY010000193         6124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_484690, whole genome shotgun sequence.
ACCESSION   DBNHBY010000193 DBNHBY010000000
VERSION     DBNHBY010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_484690"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="AC16HB_04715"
     CDS             complement(<1..2279)
                     /locus_tag="AC16HB_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5606126.1"
                     /translation="MLKLQRLLLLLFTLLLAPWAAAQTSLPTGVQRAVEFEGITEYRL
                     GNGLRVLLYPDSSKPTITVNVTYLVGSRMESYGETGMAHLLEHLMFKGTPKNPMIWEQ
                     YKKRGFQINGTTSYDRTNYFEVFNASDEDLRWAIETEADRMVNSFIARKDLDTEMTVV
                     RNEYEMGENDPGNVLFKRLTAVMYDWHNYSHPTIGNRSDIEHVAIENLQAFYRTWYQP
                     DNAVLLIAGKFEPAKALQWVAQYFGPIPKPTRKLPALWTVEPAQDGERSVIVRRKGDV
                     QLVVVAYHGPPALHPDKRALDFAVQVLSATPTGRLHKALVETGMATQVFGFGWTTHDP
                     GFIGFGAAVRKEQPIGPVRDKLIEIVESSLPQAPPAADEMDRVRRQAETSAERTLNDP
                     QAIGVQLSEYLALGDWRLFFQFRNAMQAVQPAEVQAAAQRYFRRDNRTVALFMPEDNP
                     QRASVPAAPELAQALQDLKPRAAVAAGEVFDPSPANIDARTKLVRIGDLAVALLPKSN
                     RGQTVNVNLNFRFGDAQSLFGREAAAQLTNAMLTRGTSRFTRQQIADERTRLKIQGGL
                     LSFRTDREHLADALRFTAHLLRDSTFPAAEFEQLKREVVVEAEEAKNDPDQRAFELLR
                     RQFNTYPKGDPRYVYSTEEWIDAVSRTTLEDVKAFHREFFGTARGEVAVIGDFDAAQI
                     EKALRDDFADFKSKAPYTRVVRDYAAIEGRRLTVNTPDKENAVFRARIALKMRDDDPE
                     YPALLLANYMFGGSGAGANR"
     gene            complement(2338..3216)
                     /locus_tag="AC16HB_04720"
     CDS             complement(2338..3216)
                     /locus_tag="AC16HB_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606127.1"
                     /translation="MDEFKDKVAVITGAGSGFGREFARMGASLGMRLVLADVQRDALE
                     TTLNELRFGGARAIGEVIDVADGEQVKALADRTFAEFGAAHLLFNNAGVGGGGFLWEG
                     DERDWRWVLGVNLMGVVHGIRHFVPRMIEANARGAPGHIVNTASIAGLLCPPLMGVYN
                     VSKHAVVALTETLYHDLALAKSTIGVSVLCPAFVPTGIAQSHRNRPAELAPEAAPTAS
                     QRMAQAASEKAVSSGKMTAAEVAQITFDAIRAKRFYVFTHPKIMTAVEARLASIVSGG
                     APADPYASNPSTRPAG"
     gene            complement(3223..3639)
                     /locus_tag="AC16HB_04725"
     CDS             complement(3223..3639)
                     /locus_tag="AC16HB_04725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5606128.1"
                     /translation="MRNDLDGVPFMRDLGAQLLRADKGEADIALDLAERHMNSWGVGH
                     GGVVMSLLDVAMARAGKSLVDAEQGADVGSVTVEMKTSFFRPARGRVVAHGRVLHRST
                     TLIYCEAELRDVSRHLVAKALGTIKFLNRREAANRL"
     gene            complement(3646..4425)
                     /locus_tag="AC16HB_04730"
     CDS             complement(3646..4425)
                     /locus_tag="AC16HB_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606129.1"
                     /translation="MTVRKLFDLTGRTALVTGGSRGLGLQIAEALGEMGARVVLSARK
                     AEELEAAAALLKANGVDASWVAADAAQPADLARLVDETLERLGHVDILINNAGATWGA
                     PAEDHPLDAWDKVMNLNVRGTFLLTQAIGKRSMIPRRSGRIVIVASIAGLRGNAPGTM
                     KTLAYNTSKGALVNFTRALAGEWGPYGITVNALAPGFFPSKMTRGVLQQFGADRLAER
                     APLARIGGEEDLKGAALLFASDAGRHITGQVLAIDGGVTAV"
     gene            complement(4445..>6124)
                     /locus_tag="AC16HB_04735"
     CDS             complement(4445..>6124)
                     /locus_tag="AC16HB_04735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5606130.1"
                     /translation="PHNRAADLNEPRLVGEQVQLLPDIKRALDAFIAGGFMAAAADAE
                     LGGMQLPALIEKACFAQFDAANVATAAYAMLTIGNASLLLAHASQAQIDAFARPELAG
                     RFFGTMCLSEPQAGSSLSDIRTRALPDGESQLGARYRLFGTKMWISGGEHELAANIVH
                     LVLAKVPVADGTLPPGVKGISLYIVPRVLVGADGTPGERNDVALAGLNHKMGYRGTVN
                     TLLNFGEGKFTPDGRAGAIGYRIGAEGQGLALMFHMMNEARIGVGLGATMLGYAGYRA
                     SLDYARQRPQGRPSGPAGKDPAAPQVPIVAHADVRRMLLAQKSYVEGALALNLYCARL
                     VDEEKTGASVDERKRATLLLDMLTPIAKSWPSQWCLEANNLAIQIFGGYGYTRDFPVE
                     QYWRDNRLNMIHEGTHGIQALDLLGRKVVMKDGAGLALLGVTIAATIERALAAQHPDT
                     RRFGAELQEAMQRVERVTRTLWSGGNSVAALAHATTYLQAFGHVVLAWIWLDLSLAAS
                     RAYTPAADEFLHGIWHACRYFFVYELPQVAAWLGAAEAGDTTLLDARDAWL"
BASE COUNT          943 a         2128 c         2005 g         1048 t
ORIGIN      
        1 cggttggcgc ccgcgccaga cccgccaaac atatagtttg cgagcagcag tgccggatac
       61 tcggggtcat cgtcgcgcat cttcagcgcg atgcgggcgc ggaacaccgc gttctccttg
      121 tccggcgtgt ttacggtcag acgccgaccc tcgatcgcgg cgtagtcgcg cacgacccgc
      181 gtgtatggtg ccttcgactt gaagtcggca aagtcgtcgc gcagcgcttt ctctatctgc
      241 gccgcgtcga agtcaccgat gaccgcgacc tcgcctctgg ccgtgccgaa gaattcccgg
      301 tgaaacgcct tcacgtcctc gagcgtggtg cggcttacgg cgtcgatcca ctcctccgtc
      361 gaatacacat agcgcggatc gcccttcggg taggtgttga actgccgccg cagcagttcg
      421 aaggcgcgct ggtcggggtc gttcttcgcc tcctcggcct cgaccacgac ctcgcgcttc
      481 agttgctcga actcggcggc gggaaacgtc gagtcacgca gcaggtgcgc cgtgaagcgc
      541 agcgcatcgg ccaggtgctc gcggtcggtg cggaacgaca gcagcccgcc ctgtatcttc
      601 agccgcgtcc gctcgtccgc gatctgctgg cgcgtgaacc ggctcgtgcc tcgcgtcagc
      661 atcgcgttgg tgagctgcgc ggccgcttcg cgtccgaaca gcgactgcgc gtcgccaaat
      721 cggaagttca ggttgacgtt gaccgtctgg ccgcgattgg acttgggcag cagtgcgacc
      781 gccagatcac cgatgcgcac gagcttcgtg cgtgcatcga tattggcagg cgacgggtcg
      841 aacacctcgc cggccgcgac cgcggcccgt ggcttcaggt cctgcagcgc ctgcgcgagt
      901 tccggggccg ccggcacgct ggcgcgctgc gggttgtcct ccggcatgaa cagcgccact
      961 gtgcggttgt cgcgccggaa gtagcgctgg gcagcagcct gaacctccgc gggctgcacc
     1021 gcctgcatcg cattgcggaa ctggaagaac agccgccagt cgccgagcgc gaggtattcg
     1081 gacaactgca cgccgatcgc ctgcggatcg ttcagcgtcc gctcggccga agtctcggcc
     1141 tgccgccgca cgcggtccat ctcgtcggcc gcggggggcg cctgcggcag gctgctctcg
     1201 acgatctcga tcagcttgtc gcgtaccggc ccgatcggct gctccttgcg cacggccgca
     1261 ccgaagccga tgaagccagg atcgtgcgtg gtccagccaa agccgaacac ctgtgtcgcc
     1321 atgccggtct cgaccagcgc cttgtgcagg cggcccgtcg gcgtggcact gagcacctga
     1381 acggcgaaat cgagcgctcg cttgtccggg tgcagcgcgg gaggcccgtg gtacgcgacc
     1441 acgaccaact gcacgtcacc cttgcggcgg acgatcacgc tgcgctcgcc gtcctgcgcc
     1501 ggctcgaccg tccacaatgc cggcagcttg cgcgtcggct tcggaatcgg gccgaagtac
     1561 tgcgcgaccc actgcagcgc cttggccggt tcgaacttgc cggcgatcag cagtaccgcg
     1621 ttgtcgggct ggtaccaggt gcgataaaag gcctgcaggt tctcgatcgc gacgtgctcg
     1681 atgtcgctgc gattgccgat cgtcgggtgg ctgtagttgt gccagtcgta catcaccgcg
     1741 gtcaggcgct tgaacagcac gttgccggga tcgttctcgc ccatctcgta ttcgttgcgc
     1801 accaccgtca tctcggtgtc gaggtccttc ctcgcgatga acgagttgac catgcggtcg
     1861 gcctcggtct cgatcgccca gcgcaggtcc tcgtcgctgg cgttgaacac ctcgaagtag
     1921 ttggtgcggt cgtagctcgt ggtgccattg atctggaagc cgcgcttctt gtactgctcc
     1981 cagatcatcg gattcttcgg cgtacccttg aacatcaggt gctcgagcag gtgcgccatg
     2041 ccggtttcgc cgtagctctc catccgcgag ccgacgagat aggtcacgtt gaccgtgatc
     2101 gtcggcttgg acgaatccgg gtacagcagc acgcgcagcc cgttgccgag ccggtattcg
     2161 gtgatgcctt cgaactcgac cgcgcgctgc acgccggtcg gcaatgaagt ctgcgcagcg
     2221 gcccatggcg cgagcagcag cgtgaagagt agtagcagca gtcgttggag cttcagcatg
     2281 gtccttcccg gcgcgtgagg ccgttcgacc gccgcgcccg gtatcggttc atcgcggtca
     2341 acctgccggc cgcgtcgatg ggttggaggc gtagggatcg gccggcgcac cgccagacac
     2401 gatcgacgcc aagcgcgcct ccacggccgt catgatcttc gggtgcgtga atacgtagaa
     2461 gcgcttggcg cggatcgcat cgaaggtgat ttgcgccact tccgccgcgg tcatctttcc
     2521 cgacgacacg gccttttccg aggcagcctg cgccatgcgc tgcgacgcgg tcggcgccgc
     2581 ctcgggggcc agttcggccg ggcggttgcg atgcgactgc gcgatcccgg tcggcacgaa
     2641 tgccggacac aggaccgaca cgccgatcgt cgatttcgcg agcgccagat cgtgatacag
     2701 cgtttcggtc agtgcgacca ccgcgtgctt ggatacgttg tacacgccca tcagaggggg
     2761 acacagcagg ccggcgatcg acgcggtgtt gacgatgtgt cccggcgcgc cgcgcgcatt
     2821 ggcctcgatc atgcgcggca cgaaatggcg aatgccgtgc acgacgccca tcaggttcac
     2881 gccgagcacc cagcgccagt cgcgctcgtc gccttcccac aggaacccgc cgccaccgac
     2941 gcccgcgttg ttgaacagca gatgcgccgc gccgaactcg gcgaaggtgc gatccgcgag
     3001 cgccttgacc tgctcgccgt cggcgacgtc gatgacttcg ccgatcgctc gcgcgccgcc
     3061 gaagcgcaac tcgttgagcg tggtttccag cgcgtcgcgc tggacgtcgg ccagcaccag
     3121 ccgcatgccg aggctcgccc ccatgcgcgc gaactcgcgc ccgaaaccgc tgcccgcgcc
     3181 ggtgatcacg gcgaccttgt ccttgaattc gtccatcgtt tcctagagtc ggttggcggc
     3241 ttcgcgccga ttgagaaact tgatcgtgcc cagcgccttg gcgaccaggt ggcgcgatac
     3301 atcgcgcagt tccgcctcgc agtagataag cgtcgtcgac cggtgcagca cgcgcccatg
     3361 agcgacgacg cgcccgcgcg ccgggcggaa gaagctcgtc ttcatttcca cggtgacgct
     3421 gccgacgtcg gcgccctgct ccgcgtcgac cagcgacttg ccggcgcgcg ccatcgccac
     3481 gtccagcagc gacatcacca caccgccgtg acccacgccc cagctgttca tgtgccgctc
     3541 ggccaggtcg agcgcgatgt ccgcctcgcc cttgtcggcg cgcagcagtt gcgcgcccag
     3601 gtcacgcatg aacggcacgc cgtcgaggtc gttccgcatc gatgctcaca ccgcggtcac
     3661 gccgccgtcg atcgccagca cctggcccgt gatgtggcgc ccggcgtcgg aggcgaacag
     3721 cagcgccgcg cccttcagat cctcctcgcc gccgatccga gcaaggggtg cgcgctccgc
     3781 gagcctgtcc gcgccgaact gctgcagcac gccgcgcgtc atcttcgaag ggaagaaccc
     3841 cggcgccaac gcattgacgg tgattccgta gggtccccac tcgcccgcca gcgcacgcgt
     3901 gaagttgacc agcgcaccct tgctggtgtt gtaggcaagc gtcttcatcg tgccgggggc
     3961 gttgccgcgc agcccggcga tcgacgcgac gatcacgatc cggccggagc ggcgcgggat
     4021 catcgagcgc ttgccgatcg cctgtgtcag caggaacgtg ccgcgcacgt tcaggttcat
     4081 caccttgtcc caggcgtcga gcggatggtc ctcggccggg gcgccccagg ttgcaccggc
     4141 attgttgatc aggatatcga catggcccag gcgttcgagc gtctcgtcga ccaggcgcgc
     4201 aaggtcggca ggctgcgcgg cgtccgcggc gacccagctc gcatcgacgc cgtttgcctt
     4261 gagcagcgcg gcggcggctt ccagttcctc ggccttgcgc gcggacagca cgacgcgcgc
     4321 acccatctca ccgagcgctt cggcgatctg caggccgagc ccgcgcgagc cgccggtcac
     4381 gagcgcggtg cgaccggtca ggtcgaagag tttgcgaacg gtcatgtttg cctcgcgaga
     4441 ttcgtcacag ccacgcgtcg cgcgcatcga gcagggtcgt gtcgccggcc tcggcggccc
     4501 cgagccaggc ggccacctgc ggcagctcgt acacaaagaa gtagcggcac gcatgccaga
     4561 tcccgtgcag aaactcgtcg gccgccggcg tgtaggcgcg cgacgcagcc agactcaggt
     4621 ccagccagat ccacgccagc accacgtggc cgaacgcctg caggtacgtt gtcgcgtgcg
     4681 cgagcgcggc cacggagttg cctcctgacc acaacgtgcg ggtgacgcgc tcgacccgct
     4741 gcatggcttc ctgcagttcg gcgccgaagc gtcgggtgtc cggatgctgc gcggccaatg
     4801 cgcgctcgat cgtggccgcg atcgtcacgc cgagcagcgc gagcccggcg ccgtccttca
     4861 tcacgacctt gcgcccgagc aggtcgagcg cctgaatgcc gtgcgtgccc tcgtggatca
     4921 tgttcaggcg gttgtcgcgc cagtattgct cgaccggaaa gtcacgcgtg tagccgtagc
     4981 caccgaagat ctggatcgcg agattgttgg cctccaggca ccactgcgac ggccagctct
     5041 tggcgatcgg cgtcagcatg tcgagcagca acgtcgcccg cttgcgctcg tccacgctcg
     5101 caccggtctt ctcctcgtcg accaggcgcg cgcaatagag gttcagcgcc agcgcgccct
     5161 cgacatacga cttctgcgca aggagcatgc ggcgcacgtc ggcgtgcgcg acgatcggca
     5221 cctgcggcgc tgcgggatcc ttgcccgcag gcccactcgg gcggccctgc ggccgctggc
     5281 gcgcgtagtc aagcgacgcg cgataccctg catagcccag catcgtcgcg ccgagaccca
     5341 cgccgatgcg cgcctcgttc atcatgtgga acatgagcgc aagcccctgc ccttcggcgc
     5401 cgatccgata gccgatcgcg ccggcgcggc cgtcgggcgt gaactttccc tcgccgaaat
     5461 tcagcagcgt gttgaccgtg ccacggtagc ccatcttgtg gttcagcccc gcgagcgcca
     5521 cgtcgttgcg ttcgccgggc gtgccatcgg cgccaaccag cacgcgcggc acaatgtaga
     5581 gcgagatccc tttcactccc ggcggcagcg ttccgtcggc cacggggact ttcgccagca
     5641 ccagatgcac gatgttcgcg gccagttcgt gctcgccgcc cgagatccac atcttggtgc
     5701 cgaacagccg gtagcgtgca cccaactgcg attcgccgtc gggcagcgca cgcgtgcgga
     5761 tgtccgacag cgaggagcct gcctgcggct ccgacaggca catcgtcccg aagaaccggc
     5821 ccgcaagttc cggccgcgcg aaggcgtcga tctgcgcctg tgaggcgtgc gccagcaaca
     5881 ggctggcgtt gccgatcgtc agcatcgcgt acgcggccgt ggcgacgttg gcggcatcga
     5941 actgcgcgaa gcaggctttc tcgatgagcg cgggcagctg catgccaccg agttcggcat
     6001 cggctgcagc agccatgaag ccgccggcga tgaacgcgtc gagtgcgcgc ttgatgtcgg
     6061 ggagaagttg cacctgctcg ccaacgaggc gcggctcgtt caaatcggct gcccggttat
     6121 gcgg
//
LOCUS       DBNHBY010000194         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1567184, whole genome shotgun sequence.
ACCESSION   DBNHBY010000194 DBNHBY010000000
VERSION     DBNHBY010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1567184"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="AC16HB_04740"
     CDS             complement(<1..767)
                     /locus_tag="AC16HB_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HIR5606131.1"
                     /translation="MISIGTPLLWSLFAAFVLVALLIDFFALSRQGAHKVSMREAALW
                     SLIWVAVSFAFCGWLWWYLGGAQGNAVADTKALEFATGYLVEKALAVDNIFVFLMLFT
                     YFSVPAEFQKRVLMIGILGALVLRAVMILIGAWLIARFDWILYIFGAFLLFTGIKMWW
                     AAGQEPDLESNPALKWMRRRLKIAPGYDGERFFTRVDGVRMATPLAVVILLIGVVDIV
                     FAVDSIPAIFAITTDPFIVLTSNVFAILGLRAMYFLLA"
     gene            complement(1025..2038)
                     /locus_tag="AC16HB_04745"
     CDS             complement(1025..2038)
                     /locus_tag="AC16HB_04745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5606132.1"
                     /translation="MRVFQIQDDWGMDHLQIAARPDPKPGPGQVLLKMKAASLNYRDL
                     VVPNRGYGSFTGNLPLIPISDGVGEVVEVGAGVTRAKVGDRVCPCFHQNWIAGEPDLE
                     RITRTLGGPIDGAMADLMCLSEQGVAKVPAHLSDEEAATLPCSALTAWSALVTYEDLR
                     PGSRVLVQGTGGVALFALQFAKLLGAHVTVISSSDAKLARAKLLGADVGINYATTPEW
                     SKPAREASGGGGFDHIVELGGEKTLPQSLRCIRPGGTLSMIGVLSGSSLSAPLGLVVT
                     RQVRMQGITVGHRDGFEAMLRAIAQHKLRPVLDRVFTFEQLKEALAYLKSGAQFGKIC
                     IKH"
     gene            2154..>2563
                     /locus_tag="AC16HB_04750"
     CDS             2154..>2563
                     /locus_tag="AC16HB_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR5606133.1"
                     /translation="MTADLDRLRDWIGRTEERTDVVTAAPLAALSATLDRDDPAPAAG
                     DEVPPLAHWLYFLPMAKQSELGPDGHPKRGGFLPPVPLPRRMWAGGRLTFEQPLRVGD
                     AITRTSRIADVTVKSGRSGALVFVCVRHEIRTTRG"
BASE COUNT          441 a          861 c          871 g          390 t
ORIGIN      
        1 gcgagcagga agtacatcgc gcgcaggccg aggatcgcga atacgttgga tgtgagcacg
       61 atgaacgggt cggtcgtgat cgcgaagatc gccgggatcg agtcgaccgc gaacacgatg
      121 tcgacgacgc cgatcagcag gatcacgact gcgagcgggg tcgccatccg cacgccgtcg
      181 acgcgcgtga agaacctctc gccgtcgtag ccgggcgcga tcttcagacg gcggcgcatc
      241 catttcagcg caggattgga ctcgaggtcg ggctcctgcc cggcggccca ccacatcttg
      301 atccccgtga agagcaggaa cgcgccgaag atgtacagga tccagtcgaa gcgcgcgatc
      361 agccacgcgc cgatcaggat catcaccgcg cgcagcacca gcgcgccgag aatgccgatc
      421 atcagcacgc gcttctggaa ctcggccggc accgaaaagt aggtgaacag catcaggaac
      481 acgaagatgt tgtccaccgc gagcgccttc tcgaccaggt agccggtcgc gaattcgagc
      541 gccttggtgt cggcgactgc gttgccttgc gcgccgccga ggtaccacca cagccagccg
      601 cagaaggcga acgatacggc gacccagatc agcgaccaaa gggccgcttc gcgcatcgac
      661 actttgtgcg cgccctgccg gctgagcgcg aagaagtcga tcagcagggc gacgagaacg
      721 aaggccgcga acagcgacca cagcagcggc gtgccgatgg aaatcatgga gttgcctcga
      781 tcgtgtggcc aggcgcgggc cgcggggttg gcgtcgttgc tctcctggtc tggcggaccg
      841 gctgcgccgg cctgcggtac cgggcgaggg accttgcgcc tgcgcgatca ccccgccgta
      901 ttgacgacac cgcagcggga acatgagtcc gttcccgcgc tactccccgt cagaggcgcg
      961 cgcattagac cagccgtgga cgcaagccgt caacgcgcgc ggcggcggtg aaaaccctga
     1021 atccctagtg cttgatgcag atcttgccga actgcgcgcc gctcttcaga tacgccagcg
     1081 cttctttcag ttgttcgaag gtgaacacgc gatcgagcac gggccggagc ttgtgctgag
     1141 cgatcgcgcg cagcatcgcc tcgaagccgt cgcggtggcc gaccgtgatt ccctgcatac
     1201 gcacctggcg cgtcaccacc aggccgagcg gcgccgacag gctggatccg gacagcacgc
     1261 cgatcatcga cagcgttccg cccgggcgga tgcagcgcag cgattggggc agggtcttct
     1321 cgccgccgag ctcgacgatg tgatcgaagc cgccgcctcc actggcttcg cgcgccggtt
     1381 tcgaccactc gggcgtggtc gcgtaattga tgccaacgtc ggcgccgagc agcttcgcgc
     1441 gcgcgagttt cgcgtcgctc gacgaaatta ccgtcacgtg cgcgcccagc agcttggcaa
     1501 actgcagcgc gaacagcgcg acgccgccgg tgccctgcac cagcacgcgc gagccaggtc
     1561 gcaagtcctc gtaggtgacc agcgcgctcc acgcggtcag cgcggagcaa ggcagggtgg
     1621 cggcttcttc gtcggacagg tgggcgggca ccttcgccac cccctgctcg gacaggcaca
     1681 tcaggtcggc cattgcgccg tcgatcggcc cgccgagcgt gcgcgtgatg cgttccaggt
     1741 ccggctcgcc tgcgatccag ttctgatgga agcacgggca gacgcggtcg cccaccttcg
     1801 cgcgcgtgac cccggcgccg acctcgacca cctcgcccac gccgtcggag atcggtatca
     1861 gcggcaggtt gccagtgaac gaaccgtagc cgcggttcgg caccacgaga tcgcggtagt
     1921 tcagcgatgc ggccttcatc ttcagcagca cctgccctgg tccgggcttc ggatcgggac
     1981 gcgcggcgat ctgcaggtgg tccatgcccc agtcgtcctg gatctgaaag actctcacgg
     2041 tcgcctcctc gtacgagacc cgcatcatcg cgccaaacgc gcgcaaggtg gatggctcga
     2101 attgtcgttt ccgcgcggac ggtgcaagct ttcgccatgg tggaggagac gcgatgacgg
     2161 cggacctgga tcgactgcgc gactggatcg ggcgcaccga agagcggacc gacgtggtca
     2221 ccgccgcgcc gctggcggcg ctttcggcca cgctcgaccg cgacgatccc gcgcccgcgg
     2281 cgggcgacga ggtgccgccg ctggcgcact ggctgtactt cctgccgatg gcgaagcaga
     2341 gcgagctcgg gcccgatggc catccgaagc gcggtgggtt cctgccgccg gtgccgctgc
     2401 cgcgtcggat gtgggcgggc ggccggctca cgttcgagca gccgctgcgc gtcggcgacg
     2461 cgatcacgcg cacctcgagg atcgccgacg tgacagtgaa gtcggggcgc agtggtgcgc
     2521 tggtctttgt ctgcgtgcgg cacgagatcc gcacgacgcg cgg
//
LOCUS       DBNHBY010000195         3896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1025858, whole genome shotgun sequence.
ACCESSION   DBNHBY010000195 DBNHBY010000000
VERSION     DBNHBY010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1025858"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC16HB_04755"
     CDS             complement(<1..807)
                     /locus_tag="AC16HB_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606134.1"
                     /translation="MIDRGTAVLLTLPPLFWAGNAVFARMLVGEMPPLALSFARWALA
                     LVLLLPFAWRALRDHRAALRTHWRTIALIGVIGVGGYNTFQYLAVQTSTALNVTLIAS
                     STPVFVLAVGALFFGEHPRHAQWLGAALSIVGVLVVLSRGDPANLLAVSFVAGDLIML
                     AANLTWTVYTWLLRTRRPRLPFAPFLTLQMAFGVLAIAPFAAAEAALGATKIQWTVPV
                     VLVVLYVALLPSIVAYYCWDRGVAQVGAIIPVYFANLTPLFTAALSTALLG"
     gene            complement(804..1949)
                     /locus_tag="AC16HB_04760"
     CDS             complement(804..1949)
                     /locus_tag="AC16HB_04760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606135.1"
                     /translation="MPDATIDTDIVVIGAGIAGASITYELAATRRVILLERESQPGYH
                     TTGRSAAMYAPAYGNPVVRALTRASQAFYDTPPAGFTDVPLLHARGMLFIGTAERTEE
                     ARQMLAEPQLARILKAMTLDEVRAWIPILRPEACVHALLDETSQDIDANALHMGYLRA
                     FKARGGRTLTDAGVRGLEGAAGHWRVDVGTRVLRAPIVVNAGGAWVDEIAQLAGLAPL
                     GMQPLRRTALIVDAPHGVDVTRWPAVIEIAETFYFKPDAGRLLICPVDETPSPPCDAQ
                     PEEIDVAIAVDRFERATEESVKRVIRKWAGLRTFAPDRSPVAGFDPRAPGFFWMAGQG
                     GYGLQMGPALARAAAALLLDQPLPADIADEGVDPAALSPRRLLGAAP"
     gene            2039..2551
                     /locus_tag="AC16HB_04765"
     CDS             2039..2551
                     /locus_tag="AC16HB_04765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606136.1"
                     /translation="MQIRQLTPSDAAEYRALRLRALREHPEAFRSSYEEEVLKADEWS
                     ARRLAADGHGCFLGAFNAGGALIGTVGLELEPRAKLRHQGHVIGMYVAPEQAHRGVGR
                     ALLAAIIAHARALEHVEALTLTVTSSNEHAVRLYRNAGFVECGREPRALKLDGRYYDK
                     TLMFLDLRAE"
     gene            2563..3186
                     /gene="kynB"
                     /locus_tag="AC16HB_04770"
     CDS             2563..3186
                     /gene="kynB"
                     /locus_tag="AC16HB_04770"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HIR5606137.1"
                     /translation="MRRVFDISPAVRPGFPVFPGDSPFTFRWTWKLGPNCPVNVSEVS
                     MSPHTGAHTDAPLHYDDAGASIGEVALEPYLGPCRVIHAIGTAWVGPHHLARRLDGTP
                     PRVLVRTYERAPVGTWDSSFAALAPAAIDLLHEHGVRLVGIDTPSLDPEQSKTLDSHQ
                     VVRRNGMAILEGIVLDAVSEGDYELIALPLKWHGLDASPVRALLREL"
     gene            3191..>3896
                     /locus_tag="AC16HB_04775"
     CDS             3191..>3896
                     /locus_tag="AC16HB_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5606138.1"
                     /translation="MTREDCVALDRSDPLAAHRNAFALPDGVVYLDGNSLGALPRATP
                     ARVREVVEREWGEGLIRSWNAARWIEAPRRVGAKIARLIGAQPHEVICADSTSINVFK
                     VLAAALKLQAARPQVRARERRVVLSERGNFPTDLYIAQGLLALLGDRYELKLADFDEI
                     AEAIDNTVAVVLVTHVNFQTGAMHDMATVTRRARNAGALMIWDLAHSAGAVPVNLDEC
                     EADFAVGCGYKYLNGGP"
BASE COUNT          624 a         1355 c         1366 g          551 t
ORIGIN      
        1 gccgagcagc gcggtcgaca gcgccgccgt gaacagcggc gtcaggttgg cgaaatacac
       61 gggaatgatc gcgccgacct gcgcgacacc gcggtcccag cagtagtacg cgacgatcga
      121 tggcagcagc gcgacgtaca acaccaccag cacgaccggc acggtccact ggatcttcgt
      181 cgcgcccagt gccgcttccg ccgctgcgaa cggcgcgatc gccagcacgc cgaacgccat
      241 ctgcagcgtc aggaagggcg cgaatggcag ccttggccgg cgcgtgcgca gcagccacgt
      301 atacacggtc caggtcaggt tggcggccag catgatcagg tcgccggcga cgaacgacac
      361 ggcgagcagg ttggcggggt cgccgcgcga gagcaccacc agcacgccga cgatcgacag
      421 cgccgcgccc agccactgcg cgtggcgcgg atgctcaccg aagaaaagcg caccgaccgc
      481 gagcacgaac accggcgtgc tcgacgcgat cagggtcacg ttcagcgcgg tcgaggtctg
      541 caccgcgagg tactggaagg tgttgtaccc gccgacgccg atcacgccga tcagcgcgat
      601 cgtccgccag tgtgtgcgca gcgcggcgcg atggtcgcgc agcgcacgcc acgcgaacgg
      661 cagcagcagt accagcgcga gcgcccagcg cgcgaacgac aacgcgagcg gcggcatttc
      721 gcccaccagc atccgcgcga acaccgcatt gccggcccag aacagcggtg gcagcgtcag
      781 caggaccgcg gtgccgcggt cgatcaagga gcggccccga gcagtcgccg cggcgacagc
      841 gcggccggat cgacgccttc gtccgcgatg tccgccggca gcggctgatc cagaagcagc
      901 gccgccgccg cgcgcgcgag cgccggtccc atctgcaggc cgtagccgcc ctgccccgcc
      961 atccagaaga agcccggcgc gcgcggatcg aagccggcca ccggggagcg gtcgggcgcg
     1021 aacgtgcgca gtccggccca cttgcggatc acgcgcttga ccgattcctc ggtcgcgcgc
     1081 tcgaaccggt cgaccgcgat cgccacgtcg atttcctccg gctgcgcatc gcatggcggc
     1141 gatggtgtct cgtcgaccgg gcatatgagc aggcggccgg catcgggctt gaagtagaac
     1201 gtctcggcga tctcgatcac cgcgggccag cgcgtgacat ccacgccgtg cggcgcgtcg
     1261 acgatcagcg cggtgcgccg gagcggctgc atgccgagcg gggcgagccc agcgagctgc
     1321 gcgatctcgt cgacccaggc gcccccggcg ttgaccacga tcggcgcgcg cagcacacgc
     1381 gtaccgacgt cgacgcgcca gtggccggct gcgccctcga gcccgcgcac gccggcgtcg
     1441 gtcagcgtcc gcccgccccg cgccttgaac gcgcgcagat aacccatgtg cagcgcgttg
     1501 gcatcgatat cctgcgaggt ttcgtcgagc agcgcgtgca cgcatgcctc gggccgcagg
     1561 atcggaatcc aggcccgcac ttcatccagc gtcatcgcct tcaagatgcg cgcgagctgc
     1621 ggctcggcaa gcatctgccg cgcttcttcc gtgcgctccg cggtgccgat gaacagcatg
     1681 ccgcgggcat gcagcagcgg cacgtcggtg aagccggcgg gcggtgtgtc gtagaacgcc
     1741 tggctggcgc gcgtcagcgc acgcacgacc ggattgccgt aggccggcgc atacatcgcg
     1801 gccgagcgcc cggtcgtgtg ataacccggc tgcgattcgc gttcgagcag gatcacgcgg
     1861 cgtgtcgccg cgagttcata ggtgatcgag gcaccggcga tgccggcgcc gatcacgacg
     1921 atgtcggtgt cgatggtcgc atctggcatg gccgaagatt accgaagcct tgggcctgtt
     1981 tacgcttggc tcgcggatgc agcgatccaa gccttagcag gccctagaat tccttcgcat
     2041 gcagatacgc cagctgacgc cctccgatgc cgccgagtat cgcgcgctgc ggctgcgcgc
     2101 actgcgcgaa cacccggagg cgtttcgctc cagctacgag gaggaggtgc tgaaggcgga
     2161 cgagtggtcg gcccgccgcc tcgccgctga cgggcacggt tgcttcctcg gcgcgttcaa
     2221 cgccggcggc gcgctgatcg gcacggtggg ccttgaactc gagccgcgcg cgaagttgcg
     2281 ccaccagggg cacgtgatcg gcatgtacgt tgccccggaa caggcacatc gcggcgtcgg
     2341 acgggcgctg ctcgcggcca tcatcgcgca cgccagggcg ctcgagcatg tcgaagcgct
     2401 gacgctgacc gtgaccagca gcaacgagca cgcggtccgg ctgtaccgca atgccggctt
     2461 cgtcgaatgc ggccgcgagc cgcgcgcgct gaagctcgac ggccgctatt acgacaagac
     2521 actgatgttc ctcgacctgc gcgcggagta gatttgcgcg ccatgcgtcg tgtctttgac
     2581 atctcgcccg cggtacgtcc ggggtttccg gtgtttcccg gcgactcgcc gttcacgttc
     2641 cgctggacgt ggaagctcgg cccgaactgc ccggtcaacg tgtcggaggt gtcgatgtcg
     2701 ccgcacacgg gcgcgcacac cgacgcgccg ctgcactacg acgacgccgg tgcgtcgatc
     2761 ggcgaggtcg cgctcgaacc gtatctcgga ccgtgccgcg tgatccatgc gatcggcacg
     2821 gcatgggtcg ggccgcacca tctggcgcgc cgcctcgacg gcacaccgcc gcgcgtgctg
     2881 gtgcgcacgt acgagcgcgc gccggtcgga acgtgggatt cgagtttcgc cgcgttggcg
     2941 ccggcggcca tcgacctgct gcacgagcac ggcgtgcgac tcgtcggcat cgacacgccg
     3001 tcgctcgacc ccgagcagag caagacgctc gacagccatc aggtcgtgcg ccgcaacggc
     3061 atggcgatcc tcgagggcat cgtgctggac gccgtgtccg agggcgatta cgaactgatc
     3121 gcgctgccgt tgaaatggca cgggctggac gcgagccccg tgcgcgcgct gctgcgggag
     3181 ctatgagtcg atgacccgcg aagactgcgt cgccctcgac cgcagcgatc cgctggcggc
     3241 gcaccgcaac gcgttcgcgc tgcccgatgg cgtggtctac ctggacggca attcgctcgg
     3301 cgcgctgccg cgcgcaacgc ccgcgcgtgt gcgcgaagtc gtcgagcgcg aatggggcga
     3361 gggcctgatc cgctcgtgga acgcggcgcg ctggatcgaa gcgccgcggc gcgtcggcgc
     3421 caagatcgcg cggctgatcg gcgcccagcc gcacgaggtg atctgcgccg actcgacgtc
     3481 gatcaacgtg ttcaaggttc tggcggcggc actgaagctg caggcggcgc ggccgcaggt
     3541 gcgtgcgcgc gagcgacgcg tggtgctgtc ggagcgcggc aatttcccga ccgatctgta
     3601 catcgcgcag ggactgctcg cgctgctcgg cgaccgctac gagctgaagc tcgcggattt
     3661 cgacgagatc gccgaggcga tcgacaacac ggtcgcggtc gtgctggtca cgcacgtcaa
     3721 ctttcagacc ggcgcgatgc acgacatggc gaccgtcacg cgccgcgcgc gcaacgccgg
     3781 cgcgctgatg atctgggacc tggcgcactc cgccggcgcg gtgccggtga atctcgacga
     3841 atgcgaggcc gatttcgcgg tcgggtgcgg ctacaagtac ctgaacggcg gcccgg
//
LOCUS       DBNHBY010000196         3406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_11199, whole genome shotgun sequence.
ACCESSION   DBNHBY010000196 DBNHBY010000000
VERSION     DBNHBY010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_11199"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1483)
                     /locus_tag="AC16HB_04780"
     CDS             complement(236..1483)
                     /locus_tag="AC16HB_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5606139.1"
                     /translation="MLIASLLLIGIVLVVTALAEPAVRRLPLSPALVYLASGWSAAWI
                     ASPPLPEADLERHATLLRVASEIAVLVSLFAIGLKIRMPATWRAWRVPALLATSSMLW
                     SVALAAALAWLLLPLDWPLALLLAAILAPTDPVLASDVQVHGQHDRDALRLSLTAEGA
                     LNDGTAYPIVMLALGLLGLHELGEYGMQWALRDVLWSMVAGVALGVACGRAIGWAMLR
                     RLRAGSSSDWDELLYLGAIALVYGLALAAQASAFLAVLTAGVTLLRRHPAHAPEPEAQ
                     QLGQQMQAFGARCERLVEVLMVLVIGAALTRVQWSSGVIAFACAMLLAVRPLSVLVGV
                     PPRELPFTQRGLIAWFGIRGVGSIFYLGLALQYGIADAGAQPVISACLATLALSIALH
                     GVSATPLMNWYQQRRATRMPRLR"
     gene            complement(1580..2044)
                     /locus_tag="AC16HB_04785"
     CDS             complement(1580..2044)
                     /locus_tag="AC16HB_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein"
                     /protein_id="HIR5606140.1"
                     /translation="MRSVVMFGPAMACAALLAACAGPIGPAAADPSGSVVSGTDRYAG
                     YGRVARIDAVPSERRTSGAGALIGGVLGAVVGNQIGGGTGRTAATVAGAVGGAVVGNQ
                     VEKNQSQPAVAAYDVTVRLDSGESRVITVSDPGGLAIGDRVRIEGYNIVRLG"
     gene            complement(2067..2390)
                     /locus_tag="AC16HB_04790"
     CDS             complement(2067..2390)
                     /locus_tag="AC16HB_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5606141.1"
                     /translation="MKHLHAIAAALTAASLITLPGCAVTRGQSTVGEYIDDTAITTSV
                     KARFAENKDVDATSIRVETLNGTVLLSGFAKSAKERDSAETIARAVKGVKSVKNEIVI
                     RPPAG"
     gene            complement(2599..>3406)
                     /locus_tag="AC16HB_04795"
     CDS             complement(2599..>3406)
                     /locus_tag="AC16HB_04795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5606142.1"
                     /translation="VSRVGIDALAVLSLLVLFLYVRQARAIGEMRARQAREIQAERDQ
                     LEREVGRRTAQLTELARHLQSAREDERRRLARELHDELGALLTAAKLDAARIRSRLGA
                     REPEALERLDHLNQALNDGIALKRRIIEDLRPSSLDNLGLVAALEILAREFEQTAKVV
                     VRCRLESVRLAPDAELTVYRLVQEAFTNIAKYARARAVAVSLAARDGMAEVEVVDDGV
                     GFDPDAHTQSAHGLLGMRYRVESDGGTLAIDATPARGTRIRARIPEQRVA"
BASE COUNT          552 a         1239 c         1155 g          460 t
ORIGIN      
        1 tcgggattcg cgtactcgaa gtgggtgaag ccgcgcgggt acttcggctc gccgaaggcc
       61 gcgtaggcgt gtacccattg gccgtcggcg gcaggcgcga gtgccggcac cgcgagcagc
      121 aggcaaaacg atagaagaag gcgcatcggc gggtcggtcg aaagcgggcg gatgatcgcc
      181 cacggcctgc gtgccgacaa gcgcgccggg agccggcacc tccgccgccg cccggtcagc
      241 gcagccgagg catgcgtgtc gcgcggcgct gctgatacca gttcatcagc ggcgtggccg
      301 agacaccgtg cagcgcaatc gacagggcga gcgtggccag gcaggcggat atcaccggct
      361 gcgcgccggc atcggcgatt ccgtactgaa gcgcaagccc gagatagaag atcgacccga
      421 cgccgcggat tccgaaccag gcgatcaggc cacgctgcgt gaacggcagt tcccggggcg
      481 gcacgccgac cagtacgctg agcggccgaa ctgccagcag catcgcgcag gcaaaggcga
      541 tgacgccgct gctccactgg acgcgggtca gggccgcgcc gatgacgagc accatcagga
      601 cttcgaccag gcgttcgcag cgggcaccga aggcctgcat ctgctggccc aactgctgcg
      661 cctccggctc gggtgcatgc gcagggtgtc tgcgcaacag cgtgacgcca gccgtaagga
      721 cggcgagaaa cgccgatgcc tgtgcggcga gcgcaagacc gtagacgagc gcgatcgcgc
      781 caaggtacag cagctcgtcc cagtcggacg acgatcccgc ccggagccgg cgcagcatcg
      841 cccagccgat cgcgcgtccg cacgcgacgc cgagtgcaac gccggcgacc atcgaccaca
      901 gcacatcgcg cagcgcccac tgcatcccgt attcccccaa ctcatgcagt ccgagcagcc
      961 cgagcgcaag catcacgatc ggatacgcgg tgccgtcgtt cagcgcgccc tcggcggtca
     1021 gcgacagccg cagggcgtca cgatcgtgct ggccgtgcac ctgcacgtcg gaagcgagta
     1081 ccggatcggt gggtgcgagt atggccgcga gcagcagcgc gagcggccag tcgagcggca
     1141 gcagcagcca tgccagcgcc gccgccagcg ccaccgacca cagcatgctc gacgtggcga
     1201 gcagcgccgg cacgcgccat gcgcgccagg tggcgggcat gcggatcttc aacccgatcg
     1261 cgaacagcga taccagcacc gcgatctcgc tcgccacgcg cagcagcgtc gcatggcgct
     1321 ccagatcggc ctccggcaac ggcggcgacg cgatccacgc cgcgctccag cctgacgcga
     1381 ggtacaccag cgcgggcgag agcggcagcc ggcgcacggc gggctccgcc agcgccgtga
     1441 ccaccagcac gattccgatc agcagcagcg aagcgatcag cacgtgcgcg cgcgccgcgg
     1501 cgccttcaaa ccgcaaaggg ccggggccgc gcgacggcac gcggccggtg cgagccgaac
     1561 cgtcgctcga cccgcgcgat cagccaagac ggacgatgtt gtagccctcg atacgcacgc
     1621 ggtcgccaat ggccaaaccg cccggatcgg acaccgtgat cacgcgcgac tcgccgctgt
     1681 cgagccgcac cgtcacgtcg tacgcggcca ccgccggctg cgactggttc ttctcgacct
     1741 gattgccgac caccgcgccg ccgacggcac cggccaccgt cgccgcggtg cgtcccgtgc
     1801 caccgccgat ctggttgccg acgaccgcgc cgagcacgcc gccgatcaag gcgccggcac
     1861 cggacgtgcg gcgctcgctc ggcaccgcgt cgatgcgagc cacgcggccg tagcccgcat
     1921 agcggtccgt gccgctgacc acgctgccgg acggatcggc agcagccggc ccgatcgggc
     1981 cggcgcaggc ggccagcagc gccgcgcacg ccatggccgg tccaaacatg acgacagatc
     2041 tcatccaatg catcgttcgt ctccggttaa ccagccgggg gccggatcac gatctcgttc
     2101 ttgaccgact tcacgccctt gacggcgcgc gcaatggtct ccgcgctgtc ccgctccttc
     2161 gcgctcttgg cgaagcccga cagcagcacg gtgccgttca gcgtctcgac ccggatcgac
     2221 gtggcgtcga cgtccttgtt ctccgcgaac ctcgccttca ccgacgtggt gatcgccgta
     2281 tcgtcgatgt actcaccgac ggtcgactgg ccgcgcgtca cggcgcagcc cggcagcgtg
     2341 atcagtgatg ccgcggtcag cgcggcggcg attgcatgca gatgcttcat tgcgtttctc
     2401 ctcgcttggt gtagccggcg gagccccttc acctgcggtg cgggacgacc agaccccggc
     2461 gttccggttg gctacgcagc agcgtgctgc gatgcaggca gactacgcag cgccatcgac
     2521 gaccgccatg ggacagccgg ccggtgtcgt gtcggtcgac ggcccgatcc tttgtaggac
     2581 cagaccgaca gtccgctatt aggccacgcg ctgctcggga atcctcgcgc ggatgcgcgt
     2641 gccgcgcgcc ggcgtggcgt cgatcgccag cgtcccgccg tccgactcga cgcggtagcg
     2701 catgccgagc aggccgtgcg ccgactgcgt gtgcgcgtcg ggatcgaagc cgacgccgtc
     2761 gtcgacgacc tcgacctcgg ccatgccgtc gcgtgcggcg aggctgactg cgacggcgcg
     2821 tgcgcgcgcg tacttggcga tattggtgaa tgcctcctgc acgagccggt aaacggtcag
     2881 ctctgcgtct ggtgccaggc gcaccgattc gaggcggcat cgcacgacga ccttcgccgt
     2941 ctgttcgaac tcgcgcgcca ggatctccag cgcggcgacc aggcccaggt tgtcgagcga
     3001 cgacggccgc aggtcctcga tgatccgccg cttcagcgcg atgccatcgt tcagcgcctg
     3061 gttcagatgg tccagtcgct ccagcgcctc gggctcgcgc gcgccgagcc gcgagcggat
     3121 gcgtgccgcg tcgagcttgg ccgcggtcaa cagcgcaccg agttcgtcat gcagttcgcg
     3181 cgcgagtcga cggcgctcgt cctcgcgcgc cgactgcagg tgccgcgcca gctcagtgag
     3241 ctgcgcggta cggcgcccga cctcgcgctc gagttgatcg cgctcggcct ggatttcgcg
     3301 cgcctggcgc gcccgcatct cgccgatcgc gcgcgcctga cgcacataga ggaacagcac
     3361 gagcaggctc agcaccgcca gcgcgtcgat gcccacgcgg ctgacg
//
LOCUS       DBNHBY010000197         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1769934, whole genome shotgun sequence.
ACCESSION   DBNHBY010000197 DBNHBY010000000
VERSION     DBNHBY010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1769934"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16HB_04800"
     CDS             complement(<1..560)
                     /locus_tag="AC16HB_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5606143.1"
                     /translation="MSAAYVGTSGWSYDFWRTDFYAGVPRRAWLAHYATRFNAVEVNA
                     SFYHALRPAVLAGWFEQTPAAFRFCLKAHRWITHVERLRVDAAAIARERERAAPLEHK
                     LAVVLWQLPQWLRRDLPRLLDFALRLEAWPSVRHAIEFRDRSWFYAEVATCLRAHDIA
                     AVQSDAADWPRWDAVTARFVYVRLHG"
     gene            complement(557..1072)
                     /locus_tag="AC16HB_04805"
     CDS             complement(557..1072)
                     /locus_tag="AC16HB_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606144.1"
                     /translation="MKITINRAPVLTLWAAVVAERLGFDWDEAVTLGRTVAGLNAYAK
                     GKALGIFHPRPKELKEQRKALAHGAELHVDLLHRAVPVVRTPDGLRAVSKGKPVDPAS
                     VERYLQAKFGTGFDAALKAMRELARTRDADALAHEAFHLYESFRPEVPAGTRGWGAAG
                     VLDLARVLALR"
     gene            1121..1729
                     /locus_tag="AC16HB_04810"
     CDS             1121..1729
                     /locus_tag="AC16HB_04810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606145.1"
                     /translation="MARTAESTTALSFAPLTPARWHDIEQLFGPNGACGGCWCMWWKR
                     TREEFDRGKGSANKRAFRRIVAGGIEPGLIAYQDGAPIGWCAVEPRERYPVLARSPLL
                     APVDDQPVWSVTCFFVKAGHRRSGVGIALLHEAKRFVAARGGRLIEGYPKDLRGKFPG
                     ANSVWMGLATSFTAAGFKEIARRAPTRPIMRIAVRPQRSTTR"
     gene            complement(1686..>2014)
                     /locus_tag="AC16HB_04815"
     CDS             complement(1686..>2014)
                     /locus_tag="AC16HB_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606146.1"
                     /translation="ARRTYEVCAITASRVLRLCRDHVQRDADQVWQALDEGLDRLALD
                     TVHAAALRAAFSSLRASHGAYFDAMLRMHEELRAIADGVMVVAAPVEARCVSGSSTAE
                     GAPRSA"
BASE COUNT          318 a          711 c          695 g          290 t
ORIGIN      
        1 tggccgtgca gccgcacgta gacgaagcga gcggtgaccg catcccagcg cggccagtcg
       61 gctgcgtcgg actgcacggc cgcgatgtcg tgcgcgcgca ggcacgtcgc gacctcggcg
      121 taaaaccatg aacggtcgcg gaactcgatg gcatgccgca ccgatggcca cgcctcgagt
      181 cggagggcga agtccagaag gcgcggcaga tcgcggcgga gccactgggg cagttgccac
      241 agcacgaccg cgagcttgtg ttcgagtggc gcagcgcgct cgcgttcgcg cgcgatggcc
      301 gccgcatcga cacgcaagcg ctcgacgtgc gtgatccagc gatgcgcctt caggcagaaa
      361 cggaaggcgg ccggcgtttg ctcgaaccag cctgccagca ccgcgggccg cagcgcgtga
      421 tagaaggacg cgttgacctc gactgcgttg aaccgcgtcg cgtagtgcgc gagccacgcg
      481 cggcgcggca cgccggcgta gaagtcggtg cgccagaagt cgtagctcca gccgctggtg
      541 ccgacgtacg cagcgctcat ctgagcgcaa ggacccgcgc gaggtcgagc acgcccgccg
      601 cgccccagcc gcgcgtgccg gccggcacct ccgggcggaa cgactcgtag agatgaaacg
      661 cctcgtgcgc gagtgcatcg gcgtcgcgtg tgcgcgccaa ttcgcgcatc gccttcagcg
      721 ccgcgtcgaa gcccgtgccg aacttggctt gcagatagcg ctccacgctc gccggatcga
      781 ccggcttgcc cttgctcacg gcgcgcaggc cgtcgggcgt gcgcaccacc ggcaccgcgc
      841 gatgcagaag gtcgacgtga agctccgcgc cgtgcgcaag cgccttgcgc tgctccttca
      901 gctccttcgg ccgcggatgg aagatgccga gcgccttgcc cttcgcgtac gcgttcaggc
      961 ccgccaccgt gcggccgagc gtgaccgcct cgtcccagtc gaagccgagt cgttcggcaa
     1021 ccaccgccgc ccacagcgtc agcaccggcg cgcgattgat ggtgatcttc atcgcgatgt
     1081 tccagtacgc tcccgcactc cggattcccg agcgacgccc atggcccgca ctgccgaatc
     1141 tacgaccgcg ctgagcttcg cgccactgac gcccgcgcgc tggcatgaca tcgagcaact
     1201 gttcggtccc aacggcgcgt gcggcggctg ctggtgcatg tggtggaagc gcacgcgcga
     1261 ggaattcgat cgcggcaagg gctcggcaaa caagcgtgcg ttccggcgca tcgtcgctgg
     1321 cggcatcgag ccgggactga tcgcgtacca ggatggcgcg ccgatcggct ggtgcgcggt
     1381 cgagccgcgt gagcgctacc cggtgctggc gcgatcaccg ctgctggcgc cggtcgacga
     1441 tcagcccgtg tggtcggtca cgtgcttctt cgtcaaggcc ggacaccgca ggagcggcgt
     1501 gggcatcgcg ctgctgcacg aagcgaagcg tttcgtcgcc gcgcgcggcg gccggctcat
     1561 cgaaggctat cccaaggact tgcgcggcaa gtttcccggc gccaattccg tctggatggg
     1621 cctggcaacc agcttcaccg cagccggctt caaggaaatc gcgcggcgcg cgcccacccg
     1681 gccgatcatg cggatcgcgg tgcgccctca gcggtcgacg acccgctgac gcagcgggct
     1741 tcgaccggcg cagcgacgac catcacgccg tcggcgatcg cacgcaattc ttcgtgcatg
     1801 cgaagcatcg cgtcgaagta ggcgccgtgg ctcgcgcgca gcgagctgaa cgccgcgcgc
     1861 aacgcggccg catgcacggt gtcgagcgcg agccgatcga gcccctcgtc gagcgcctgc
     1921 cagacctggt ctgcatcgcg ctgcacgtga tcgcggcata gccgcagcac gcggctggcg
     1981 gtgatcgcgc acacctcgta agtgcggcgc gcaa
//
LOCUS       DBNHBY010000198         2763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1431992, whole genome shotgun sequence.
ACCESSION   DBNHBY010000198 DBNHBY010000000
VERSION     DBNHBY010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1431992"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="bcrB"
                     /locus_tag="AC16HB_04820"
     CDS             complement(<1..1178)
                     /gene="bcrB"
                     /locus_tag="AC16HB_04820"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HIR5606147.1"
                     /translation="MSTTIAVPASTKKPQNVQKEDAMWLQKELINKNYMELATARANN
                     RKVSATFVPGNLNELLMCFDFARSLPETNALQNGMRKKSGKMIMDAERDGQSEDVCTY
                     VKADLGMMMGGEIGPTGDPLPRPDVLLLSYTGCFTFMKWFELIRHKYGCETVMLHVPY
                     QGEGKIAPNMREYVVKQLKETVIPTLERISGVKFDIDRLRQYMRESAKAEEDLVKVLQ
                     SAKNRPSPIDGYFGAVYYIGPIFTAFRGTPEATEYYRVLRGEIEQRVREGKGPVTPDG
                     EMGEEKYRLIVEGPPNWTSFRDFWKMFYEEGAVVVASTYSKVGGVYDFGFRHDPEHPL
                     ESLADYCLGCYTNLNLPSRIDMICRYIDQYQADGLLINSIKSCNSFSAGQLLILREVE
                     "
     gene            complement(1175..2353)
                     /gene="bcrC"
                     /locus_tag="AC16HB_04825"
     CDS             complement(1175..2353)
                     /gene="bcrC"
                     /locus_tag="AC16HB_04825"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HIR5606148.1"
                     /translation="MTALSPVMQIVQRCEALFEDLDFNAVKQWKAAAPGRKAIGYMPV
                     YVPRELIHAAGWLPVGILGGGDQLEVIQGDAYYQSYICRIPRSTIEMGLTGRLNCLDG
                     MLFPSICDVIRNLSGMWQILFKDKYVRYLDVPQNYQDAIGGRFYIQEMQVLREDLGKL
                     RGAPITDAELNASIAVYNDNRAAVRELYAYRAQKPWQAPTAEVYLLLRAGMVLPPEEH
                     TRLVRDYIVATDAVPRPRRDNARVVVTGVFCEQPPLALIKSIEMSGCYIVDDDFMLVT
                     RWLLDDVPTNGDPLEELSKAFLHRSASTAAKYDTTREEKGVFLLKQVKTRGAEGVIFA
                     APSFCDPALLERPMLQDVLGRQKIPHTAFKYAENTGQMAPIREQAGTFADSIKLWSAA
                     "
     gene            complement(2362..>2763)
                     /locus_tag="AC16HB_04830"
     CDS             complement(2362..>2763)
                     /locus_tag="AC16HB_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5606149.1"
                     /translation="DAQFGQPEMKLGVFAPAASCLLPYRVNQPAAEDLLFSGRSIGAE
                     EAKSIGLVFRVFDDPETAALAYFQEHLAGKSAAALAYAIAAARGPLIRDTRARLAEVE
                     KLYLERLMATRDANEGLTAFLAKRKPNWEHK"
BASE COUNT          443 a          878 c          899 g          543 t
ORIGIN      
        1 ttctcgactt cgcgcaggat cagcaactgg ccggcggaaa agctgttgca gctcttgatc
       61 gagttgatca gcagcccgtc ggcctggtac tggtcgatgt acctgcagat catgtcgatc
      121 cgcgacggca ggttcagatt cgtgtagcag ccgaggcagt agtcggcgag cgattcgagc
      181 ggatgttccg gatcgtggcg gaagccgaag tcgtagacgc cgccgacctt ggagtaggtg
      241 cttgcgacga cgaccgcgcc ttcttcatag aacatcttcc agaagtcgcg gaagctggtc
      301 cagttgggcg ggccttcgac gatcagtcga tacttctctt cccccatctc gccgtccggc
      361 gtgacggggc ccttgccttc gcgcacgcgt tgctcgatct cgccgcgcag cacgcggtag
      421 tactcagtgg cttccggcgt gccccggaac gcggtgaaga tcgggccgat gtaatagacc
      481 gcgccgaagt agccatcgat cggcgaaggc cggttcttcg ccgactgcag gaccttgacc
      541 aggtcttcct cggccttggc cgattcgcgc atgtactggc gcagccggtc gatgtcgaac
      601 ttcacgccgg agatgcgttc cagcgtcggg atcacggtct ccttcaactg cttgaccacg
      661 tactcgcgca tgttcggtgc gatcttgcct tcaccctgat agggcacgtg cagcatcacc
      721 gtttcgcagc cgtacttgtg gcggatcagc tcgaaccact tcatgaacgt gaagcagccg
      781 gtgtacgaca gcagcagcac gtcgggacgc ggcagcgggt cgccggtggg tccgatctcg
      841 ccgcccatca tcatgccgag gtcggccttg acgtaggtgc agacgtcctc gctctggccg
      901 tcgcgctcgg cgtccatgat catcttgccg ctcttcttgc gcattccgtt ctgcagtgcg
      961 ttggtctcgg gcaggctgcg cgcgaagtcg aagcacatca ggagttcatt cagatttccc
     1021 ggcacgaagg tcgccgagac cttgcggttg ttggcgcgcg cggtggcgag ctccatgtag
     1081 ttcttgttga tcagttcctt ctgcagccac atcgcgtcct ctttctggac gttctgcggc
     1141 ttcttggtcg aagccggtac tgcgatcgtc gtgctcatgc ggcactccac aatttgatcg
     1201 aatcggcgaa ggtgcccgcc tgctcgcgga tcggggccat ctgcccggtg ttctcggcat
     1261 acttgaacgc ggtgtgcgga atcttctgtc ggcccagcac gtcctgcagc atcgggcgct
     1321 cgagcagcgc cggatcgcag aacgatggcg cggcaaagat cacgccttcg gcgccgcgtg
     1381 tcttcacctg tttcagcagg aacacgcctt tctcctcgcg cgtcgtgtcg tacttggccg
     1441 cggtcgacgc cgagcggtgc aggaacgcct tggacagctc ctccagcgga tcgccgttgg
     1501 tcggcacgtc gtcgagcagc cagcgcgtga ccagcatgaa gtcgtcatcg acgatgtagc
     1561 agcccgacat ctcgatcgac ttgatcagcg ccagcggcgg ctgctcgcag aagacgcccg
     1621 tcaccaccac gcgcgcgttg tcgcgcctgg gtcgcggcac ggcatcggtc gcgacgatgt
     1681 agtcacgcac gagtcgcgtg tgttcctcgg gcggcagcac catgccggcg cgcagcaaga
     1741 ggtagacctc ggcggtgggc gcctgccacg gcttctgggc gcgataggcg tacagctcgc
     1801 gcacggccgc gcggttgtcg ttgtagaccg cgatcgacgc gttgagctcc gcatcggtga
     1861 tcggcgcgcc gcgcagcttg cccagatctt cgcgcagtac ctgcatctcc tggatgtaga
     1921 agcgcccgcc gatggcgtcc tggtagttct gcggcacgtc gaggtagcga acgtacttgt
     1981 ccttgaacag gatctgccac atgcccgaca ggttgcggat cacgtcgcag atcgacggga
     2041 acagcatgcc gtcgaggcag ttcaggcgcc cggtcaggcc catctcgatc gtcgagcgcg
     2101 gaatgcggca gatgtagctc tggtagtagg cgtcgccctg gatgacttcg agttgatcgc
     2161 cgccgccgag gatgccgacc ggaagccagc cggcggcatg gatcaattcg cgcggcacgt
     2221 acaccggcat gtagccgatc gccttgcgac cgggcgccgc cgccttccac tgcttgacgg
     2281 cgttgaaatc gaggtcttcg aacagcgcct cgcagcgctg cacgatctgc atcacgggag
     2341 agagggcggt catcggtact cctacttgtg ctcccagttg ggtttgcgct tggcaaggaa
     2401 cgcggtcagt ccctcgttgg catcgcgcgt ggccatcagg cgttcgagat agagcttttc
     2461 cacttccgcg agccgcgcgc gcgtgtcgcg gatcagcggg ccgcgcgcgg cggcgatggc
     2521 gtacgcgagt gcggcggcgc tcttgcccgc gaggtgctcc tggaagtagg cgagcgccgc
     2581 ggtctccggg tcgtcgaaca cccgaaagac gaggccgatc gacttcgctt cctcggcgcc
     2641 gatcgagcgg ccggagaaca gcagatcctc cgccgcgggc tggttcacgc gatagggcag
     2701 caggcacgac gccgccggtg cgaacacgcc gagcttcatc tcgggctggc cgaactgcgc
     2761 gtc
//
LOCUS       DBNHBY010000199         4692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_756091, whole genome shotgun sequence.
ACCESSION   DBNHBY010000199 DBNHBY010000000
VERSION     DBNHBY010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_756091"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16HB_04835"
     CDS             complement(<1..308)
                     /locus_tag="AC16HB_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HIR5606150.1"
                     /translation="MRALMLTPLVAAVLAACSVAPTYQRPDANVGAAFKETVPESALW
                     KRAEPGEATARGQWWTIFDDRKLDELQADAARANPTLKAGLARLAQARAAAGIAESQR
                     "
     gene            complement(321..3512)
                     /locus_tag="AC16HB_04840"
     CDS             complement(321..3512)
                     /locus_tag="AC16HB_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5606151.1"
                     /translation="MKLSHYFIDRPILAGVLSIVIFLAGLLAIFKLPISEYPEVTPPN
                     VVVVANYPGANPKVVAETVATPLEEAINGVEGMLYMGSQATSDGRLTLTVTFKLGVDP
                     DLAQQLVQNRVQQALPRLPEEVRRLGVITSKRSPDLLMVVHLTSPNERYDANYLRNYA
                     VLNVKDVLGRIPGVGDALVFGAGDYAMRAWLDPDKVAARNLTATDVARAMRDQNANVA
                     AGVIGAPPSLPGVDYQLAVNARGRLATDEEFGDIVIKTSADGAVTRLRDVARIELGAA
                     DYSVRSLLNNNPAVAVAIFQAPGSNALAIADHVRAAMAELKGNMPEGVNYQIVYDTTR
                     FVQSSIEEVIKTLLEAVALVVLVVIVFLQTWRASVIPLIAVPVSIVGTFALLLAFGFS
                     INVLSLFGLVLAIGIVVDDAIVVVENVERNIAAGLSPREATIKAMNEVSGPIVAIALV
                     LIAVVVPLAFVSGLTGQFYKQFALTIAISTVISAFNSLTLSPALAAKLLRPHGAREDR
                     FTRAMNAVFGPFFRAFNRFFRRASDAYSNGVTRVLGHKASAMGVYVLLIGVAVLVFQA
                     VPRGFVPSQDKQYLVGFAQLPDGATLDRTESVIRRMSDIALKQPGVEAAVAFPGISLN
                     GFTNSPNAGVVFVTLKPFDERRAPQLSGFAVAGALNQQFMAIQDAFTMVFPPPPVQGL
                     GVIGGFKLQIEDRGALGYEALDGATKAFLGKAYQTPELAGVFSNYRINVPQLYADVDR
                     VKARQLGVSVTDVFDTMQVYLGSLYVNDFNRFGRTYQVKLQADAPFRAHAEDIGRLKT
                     RNDRGEMVPLSSLLAVSPSFGPDRVMRYNGYTAADINGGPAPGFSSGQAQAAAERIAH
                     EVLPKGISFEWTELTYQEILAGNTAIIVFPLAVLLVFPVLAALYESWTLPLAIILIAP
                     MALLAAVAGVWFTRGDNNVFTQIALVVLMGLAAKNAILIVEFARDLERAGRTIVQAAI
                     EASRLRLRPILMTSFAFIMGVIPLAFSVGAGAEMRRAIGIAVFSGMLGVTFFGLVLTP
                     VFYVLLRTLAERFTRKPAGGALPVTVHAAPRDE"
     gene            complement(3523..>4692)
                     /locus_tag="AC16HB_04845"
     CDS             complement(3523..>4692)
                     /locus_tag="AC16HB_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5606152.1"
                     /translation="LATALAIVAALGAAGYFLAPVSASPQPAPAAPMAVPVSVAAALE
                     RRITEFDEFSGRLEAIDRVDVRPRVAGYIDSIHFAPGAMVKKGDTLFVIDPKPYAAEV
                     ARAEAALAAAQAKLALATSELDRAKRLLDDRAIAQREFEERINAQREAQASIQSAQAA
                     LDIARLNLGYTRVTAPISGRVSRAEVTVGNLVAAGAGGPALTSIVSTSPMYATFEADE
                     QAYLKYAGKAHGRGARQLPVYMGLANEQGFPRKGAIEFVDNRLDPQSGTIRVRALFDN
                     ADGVLTPGMFARLKIGGAEASPAVLIDDRAVGTDQSKKFVFVVGADGKVAYREIRLGP
                     VVDGLRVVREGLAAGEQIVVNGLQRVRPGVPVAPTVVQMERKPELARSEASAARS"
BASE COUNT          747 a         1668 c         1575 g          702 t
ORIGIN      
        1 aagcgctgcg actcggcaat cccagcggcc gcgcgtgcct gcgccagtcg cgccagcccg
       61 gctttcagcg ttggattggc gcgtgccgcg tcggcctgca gttcgtccag cttccgatcg
      121 tcgaagatcg tccaccactg cccgcgcgcg gtggcctcgc ccggctcggc gcgcttccaa
      181 agcgccgatt cgggcaccgt ctccttgaac gccgcgccga cgttcgcgtc gggccgctgg
      241 tacgtcggtg cgacgctgca ggcggccagc acggccgcga ccaacggcgt cagcatcaat
      301 gctctcatct ccatcctccg ctattcgtcg cgcggcgccg cgtgcaccgt caccggcagc
      361 gcaccgccgg ccggcttgcg cgtaaagcgc tcggccagcg tgcgcagcag aacgtagaac
      421 acgggcgtca acacgaggcc gaagaacgtg acgccgagca tccccgagaa cacggcgatg
      481 ccgatcgcgc gccgcatctc ggcgccggca ccgaccgaga acgccagcgg tatcacgccc
      541 atgatgaagg cgaacgaggt catcaggatc ggccgcagcc gcagccggct cgcttcgatc
      601 gcggcctgca cgatcgtgcg gccggcgcgc tccaggtcgc gcgcgaactc gacgatcagg
      661 atcgcgttct tcgccgccag gcccatcagc accacgagcg cgatctgcgt gaacacgttg
      721 ttgtcgccgc gcgtgaacca cacgccggcg accgccgcca gcagcgccat cggcgcgatc
      781 agaatgatcg ccagcggcag cgtccagctt tcgtacagcg ccgccagcac cgggaacacc
      841 agcagcaccg cgagcgggaa cacgatgatc gcggtgttgc cggcgaggat ttcctggtac
      901 gtgagctcgg tccactcgaa cgagatgccc ttgggcagga cctcgtgcgc gatgcgctcg
      961 gccgccgcct gcgcctgtcc ggagctgaag cccggcgccg gaccgccgtt gatgtcggcc
     1021 gcggtgtagc cgttgtagcg catcacgcgg tcggggccga agctcggcga aacggcgagc
     1081 agcgaggaca gcggcaccat ctcgccgcgg tcgttgcgtg tcttcagccg cccgatgtcc
     1141 tcggcgtgcg cgcggaacgg tgcgtcggcc tgcagcttaa cctggtaggt gcggccgaag
     1201 cggttgaagt cgttcacgta cagcgacccg aggtacacct gcatcgtgtc gaacacgtcg
     1261 gtcaccgaga cgccgagctg gcgcgccttc acacgatcga cgtccgcgta cagctgcggc
     1321 acgttgatcc ggtagttgga gaacacgccc gccagttccg gcgtctggta cgccttgccg
     1381 aggaaggcct tggtcgcgcc gtcgagcgct tcgtagccga gcgcgccgcg gtcctcgatc
     1441 tgcagcttga agccgccgat cacgccgagc ccctgcaccg gcggcggcgg aaacaccatc
     1501 gtgaacgcat cctggatcgc catgaactgc tggttcagcg cgcccgcgac cgcgaacccc
     1561 gaaagctgcg gcgcgcggcg ctcgtcgaaa ggcttcaacg tgacgaacac gacgcccgcg
     1621 ttcgggctgt tggtgaagcc gttcagcgag atgccgggaa acgcgaccgc cgcctcgacg
     1681 ccgggctgct tcagcgcgat gtccgacatg cggcggatca cgctctcggt gcggtcgagc
     1741 gtggcgccgt ccggcaattg cgcgaagccg accaggtact gcttgtcctg gctcggcacg
     1801 aagccgcgcg gcaccgcctg gaacacgagc accgccacgc cgatcagcag cacgtacacg
     1861 cccatcgcgc tcgccttgtg cccgagcacg cgcgtgacgc cgttgctgta cgcatcggac
     1921 gcgcgcctga agaagcggtt gaacgcgcgg aagaacggcc cgaacaccgc gttcatcgca
     1981 cgcgtgaagc ggtcctcgcg cgccccgtgc ggccgcagca gcttggccgc gagcgccggc
     2041 gacagcgtca gcgaattgaa ggccgaaatg acggtggaga tcgcgatcgt cagcgcgaac
     2101 tgcttgtaga actgtcccgt caggccggac acgaacgcga gcggcacgac caccgcgatc
     2161 agcaccagcg cgatcgcgac gatcggcccg ctgacctcgt tcatcgcctt gatcgtcgcc
     2221 tcgcgcggcg acagtccggc ggcgatgttg cgctcgacgt tctcgaccac cacgatcgcg
     2281 tcgtcgacca cgatgccgat cgccagcacc aggccgaaca gcgacagcac gttgatcgaa
     2341 aagccgaacg cgagcagcag cgcgaaggtg ccgacgatcg acaccggcac tgcgatcagc
     2401 gggatgaccg atgcacgcca cgtctgcagg aacacgatca cgaccagcac caccagcgcc
     2461 accgcctcca gcagcgtctt gatcacctcc tcgatcgagc tctgcacgaa gcgcgtggtg
     2521 tcgtaaacga tctggtagtt gacgccctcg ggcatgttgc ccttcagttc cgccatcgcc
     2581 gcacgcacgt ggtcggcgat cgccagcgcg ttcgagcccg gcgcctggaa gatcgccacc
     2641 gccaccgccg ggttgttgtt cagcaggctg cgcaccgagt agtcggccgc gcccagttcg
     2701 atgcgcgcga cgtcgcgcag ccgcgtgacc gcgccgtcgg ccgaggtctt gatcacgatg
     2761 tcgccgaact cctcgtcggt cgccaggcgc ccgcgcgcgt tgaccgcgag ctggtaatcg
     2821 acgccgggca gcgacggcgg cgcgccgatc acgccggcgg cgacgttggc gttctggtcg
     2881 cgcatcgcgc gcgcgacatc ggtcgcggtc aggttccgcg cggcgacctt gtccggatcg
     2941 agccaggcgc gcatcgcgta gtcgccggcg ccgaagacga gcgcgtcgcc cacgccgggg
     3001 atgcggccga gcacgtcctt gacgttcagc accgcgtagt tgcgcaggta gttggcgtcg
     3061 tagcgctcgt tcggcgacgt caggtgcacg accatcagca ggtcgggcga gcgcttcgac
     3121 gtgatcacgc cgagccggcg cacctcctcc ggcagtcttg gcagcgcctg ctgcacgcgg
     3181 ttctgcacca gctgctgcgc cagatcggga tcgacgccga gcttgaacgt gacggtcaga
     3241 gtcaggcgcc catcgctggt cgcctgcgag cccatgtaca gcatgccctc gacgccgttg
     3301 attgcctctt ccagcggcgt ggcgacggtt tcagcgacca ccttcggatt ggcgcccgga
     3361 tagttggcga cgacgacaac gttcggcggc gtgacctccg gatattctga gatcggcagc
     3421 ttgaagatcg ccagcagccc ggcgaggaag atcacgatcg acagcacgcc cgcgaggatc
     3481 gggcggtcga tgaagtagtg cgacagcttc atcggccggc ccctagctgc gcgcggccga
     3541 cgcttcgctg cgcgccagct ccggcttgcg ttccatctgc accacggtcg gcgcgaccgg
     3601 cacgcccggg cgcacgcgct gcaggccatt gacgacgatc tgctcgcccg ccgccaggcc
     3661 ttcgcgcacc acgcgcagtc cgtcgacgac ggggccgagc cggatctcgc ggtaggcgac
     3721 cttgccgtcg gcgccgacca cgaagacgaa cttcttgctc tggtcggtgc cgaccgcgcg
     3781 gtcgtcgatc agcaccgcgg ggctcgcctc ggcgccgccg atcttcagcc gcgcgaacat
     3841 gccgggcgtc agcacgccgt cggcattgtc gaacagcgcg cgcacgcgga tcgttcccga
     3901 ttggggatcg agccggttgt cgacgaactc gatcgcgccc ttgcgcggga atccctgctc
     3961 gttcgccagc cccatgtaga cgggcaactg cctggcgccg cgaccatggg ccttgccggc
     4021 gtatttcaga taggcctgtt catcggcctc gaaggtggcg tacatcggcg acgtcgatac
     4081 gatcgaggtc agcgcgggtc cgcccgcgcc ggcggcgacc aggttgccga ccgtgacctc
     4141 ggcgcggctg acgcggccgc tgatcggcgc ggtcacgcgc gtgtagccga ggttcagccg
     4201 cgcaatgtcg agcgcggcct gcgccgactg gatcgacgcc tgcgcctcgc gctgcgcgtt
     4261 gatgcgctcc tcgaattcgc gctgcgcgat cgcccggtca tcgagcaatc gcttggcacg
     4321 gtcgagctct gacgtggcca gcgcgagctt cgcctgcgcg gcggcgagtg ccgcctcggc
     4381 gcgcgcgacc tcggccgcgt acggcttcgg atcgatcacg aacagcgtgt cgcccttctt
     4441 gaccatggcg ccgggcgcga agtggatcga gtcgatatag cctgccacgc gcggccgcac
     4501 atcgactcgg tcgatcgcct ccagccggcc ggagaactcg tcgaactcgg tgatgcgccg
     4561 ctcgagcgcg gcggcgaccg aaaccggcac cgccatcggt gcggccggcg caggctgcgg
     4621 cgacgcgctc accggggcaa ggaaatagcc ggcggcgccg agcgcggcga cgatggccag
     4681 tgccgttgcc ag
//
LOCUS       DBNHBY010000200         1916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_688225, whole genome shotgun sequence.
ACCESSION   DBNHBY010000200 DBNHBY010000000
VERSION     DBNHBY010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_688225"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16HB_04850"
     CDS             complement(<1..242)
                     /locus_tag="AC16HB_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR5606153.1"
                     /translation="MSTPVSALPAPDTASRRAIGVTLALLALWVVAYANLTRFADFVF
                     GLTPLERDTHLGDAVHFFLYDTPKVLLLLLLVVFAMG"
     gene            complement(250..495)
                     /locus_tag="AC16HB_04855"
     CDS             complement(250..495)
                     /locus_tag="AC16HB_04855"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5606154.1"
                     /translation="MRVQVLGSGCSKCQITARQVEEVAATMGVPIELEKVQDPAAIVA
                     MGVFSTPAIAIDGKVVHSGGVPAREKIRALLGMAVLA"
     gene            complement(578..1606)
                     /gene="arsB"
                     /locus_tag="AC16HB_04860"
     CDS             complement(578..1606)
                     /gene="arsB"
                     /locus_tag="AC16HB_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR5606155.1"
                     /translation="MSVFERYLTLWVALCIVAGIVLGQVFPAPLQALGRMEFARVNLP
                     VGLLIWVMIIPMLLKIDFGALGQVRSHVRGIAVTLAINWLVKPFSMALLAWIFIRHVF
                     APYLPADQLDSYIAGLILLAAAPCTAMVFVWSRLTNGDPYFTLTQVALNDTIMVFAFA
                     PIVALLLGVASIRVPWDTLLTSVVLYIVVPVVVAQAIRARVLARGGQAALDALLHRIQ
                     PWSVAALLATLVLLFGFQGRQILDQPLVIALLAVPILIQVFLNSGLAYLANRALGEKH
                     TVACPSALIGASNFFELAVAAAISLFGFQSGAALATVVGVLIEVPVMLLVVRVVNTSR
                     GWYERGAR"
     gene            complement(1636..>1916)
                     /locus_tag="AC16HB_04865"
     CDS             complement(1636..>1916)
                     /locus_tag="AC16HB_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HIR5606156.1"
                     /translation="FARPGAPALDFVITVCDSAAGEVCPIWPGQPITAHWGVEDPAAV
                     EGSDDDKRRAFFHAYNRLQNRIQLFLSLPLAKLDRVALTQRLKEIGKS"
BASE COUNT          359 a          656 c          637 g          264 t
ORIGIN      
        1 cccatcgcga acacgaccag caacagcagc agcagtacct tgggcgtgtc gtagagaaag
       61 aagtgaaccg cgtcgcccag gtgcgtgtcg cgttcgagcg gcgtcaatcc gaatacgaaa
      121 tcggcgaacc gcgtgaggtt cgcataagcg acgacccaca gagcgagcag tgccagcgtg
      181 acgccgatcg cgcgccggct cgcggtgtcc ggcgcgggca gcgccgagac cggtgtgctc
      241 atcggccgct caggccagca cggccatgcc gagcagcgcg cggatctttt cgcgcgccgg
      301 cacgccgccc gaatgcacga ccttgccgtc gattgcgatg gccggcgtgc tgaatacgcc
      361 catcgcgacg atcgcggccg ggtcctggac cttctcgagt tcgatcggca cgcccatcgt
      421 cgcggcgact tcctcgacct ggcgcgcggt gatctggcac ttcgagcagc cgctgccgag
      481 aacctgaact ctcatgtgcg cctccgggaa tcctgcaaag cgtattcaga ctaggccgat
      541 cgcccgggca acgattgacg ttccgcaagc ggctgcctca gcgtgcgccg cgctcgtacc
      601 agccgcggct ggtgttgacc acgcgcacga cgagcagcat caccggtacc tcgatcagca
      661 cgccgaccac ggtggcgagc gccgcgccgg actggaagcc gaacagcgaa atcgccgccg
      721 cgaccgccag ttcgaagaaa ttgctcgctc cgatcagcgc cgacgggcag gcgacggtgt
      781 gcttctcgcc cagcgcgcga ttggccaggt acgcgagtcc cgagttgagg aacacctgga
      841 tcaggatcgg caccgcgagc agcgcgatca ccagcggctg gtcgaggatc tgccggccct
      901 ggaaaccgaa cagcagcacc agtgtggcca gcagcgccgc gaccgaccac ggctggatcc
      961 ggtgcagcag cgcgtcgagc gcggcctgcc cgccgcgggc gagcacgcgc gcgcggatcg
     1021 cctgcgcgac gacgaccggc accacgatat agagcacgac cgaggtcagc agcgtgtccc
     1081 acggcacgcg gatcgacgcg acgccgagca gcagcgccac gatcggcgcg aacgcgaaca
     1141 ccatgatcgt gtcgttcagc gccacttggg tcagcgtgaa gtacggatcg ccgttcgtga
     1201 gccggctcca cacgaacacc atcgcggtgc acggcgcggc cgcgagcagg atcaggccgg
     1261 cgatgtagct gtcgagctga tcggccggca gatacggcgc gaacacatgg cggatgaaga
     1321 tccacgccag cagcgccatc gagaacggct tgaccagcca gttgatcgcc agcgtgaccg
     1381 cgattccgcg cacgtgactg cgcacctggc cgagcgcgcc gaagtcgatc ttcagcagca
     1441 tcgggatgat catcacccag atcagcagcc cgaccggcag gttcacgcgc gcgaactcca
     1501 tgcggcccag ggcctggagc ggcgcaggga acacctgtcc aagcacgatg ccggcgacga
     1561 tgcacagcgc gacccacagt gtcaggtagc gctcgaagac gctcataccc gcgatcgtgg
     1621 gcgctgccgc cgcgctcatg acttgccgat ctccttcagc cgctgcgtga gcgctacgcg
     1681 atcgagcttg gcgagcggca ggctgagaaa gagctggata cggttctgca gccggttgta
     1741 ggcatggaag aacgcgcggc gcttgtcgtc gtcgctaccc tcgactgccg cgggatcctc
     1801 gacaccccaa tgcgcggtga tcggctggcc cggccagatc gggcagactt cgccggccgc
     1861 gctgtcgcac acggtgatca cgaagtccag cgccggcgcg ccggggcgtg cgaatt
//
LOCUS       DBNHBY010000201         3152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_349873, whole genome shotgun sequence.
ACCESSION   DBNHBY010000201 DBNHBY010000000
VERSION     DBNHBY010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_349873"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16HB_04870"
     CDS             <1..188
                     /locus_tag="AC16HB_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606157.1"
                     /translation="LASKGLDTLKAGDLVSIRLPGAGGYGDPRQRDRALVQADLRDDK
                     ISRESAVRDYGLDATEP"
     gene            193..831
                     /locus_tag="AC16HB_04875"
     CDS             193..831
                     /locus_tag="AC16HB_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5606158.1"
                     /translation="MLRYHTVPVTPFQQNSSIVWCDRTMAGAVIDPGGEIARLRAAAD
                     RLGVRLEQILLTHAHIDHAGGAGALARELAVPIIGPHPGDQFWIDALAQQSQMFGLPA
                     AETFTPSRWLTDGDTVRVGNCELAVRHCPGHTPGHVVFHSVDTQRAFVGDVLFAGSIG
                     RTDLPGGDYDTLIASIVERLWPMGDDTVFIPGHGPESTFGRERRSNPFVGGT"
     gene            914..2056
                     /locus_tag="AC16HB_04880"
     CDS             914..2056
                     /locus_tag="AC16HB_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606159.1"
                     /translation="MSAIPYRSWYFPRPALAQRILALLLEGAGDPVALVGARRIGKTS
                     HLLFELMPAAQKRGLLPVYIDMFQQRADPVAAINYALQEAIDEVEVPRSRVGRRLSTA
                     VKRIGAGSFSLDLGEEPARRRPADPLLLIDWLLKTLVRTARKPVLLIFDEAQELATLA
                     DSDNTVSAIRSAITKSRNNVRVVFTGSSQERLLALFARARAALYEGASTMPFPHLGDD
                     FIAFVAQRCRELFRKRVSAAELTEAFARLHHQPRALIDLVLAYAAGDQDSLAAVLNAR
                     IEAQLTGADYSAQWAALKPLQQRICLRVLRGDDVTSVAARREYAAGTDKPEIAPGTVN
                     DALRALVASHVLHKPSGARGAYRIDDPLFAEWLRRESASLSGRGSR"
     gene            complement(2066..2311)
                     /locus_tag="AC16HB_04885"
     CDS             complement(2066..2311)
                     /locus_tag="AC16HB_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR5606160.1"
                     /translation="MALMITQDCTACDACKPPCPNEAISVGDPIYVIDALKCTECVGA
                     EDEPQCMLVCPADCIVPNPDFQESKDELLAKYQALHG"
     gene            complement(2325..>3152)
                     /gene="bcrD"
                     /locus_tag="AC16HB_04890"
     CDS             complement(2325..>3152)
                     /gene="bcrD"
                     /locus_tag="AC16HB_04890"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HIR5606161.1"
                     /translation="IGIDIGSGAVKSVLFRVEGGQQQWIAKRCERIRRRDPMTLAQEG
                     LDGVLAEARLAANDIDYIATTGEGENVKFATGHFYSMTTHARGGVFLHPGISAVVDIG
                     ALNGRAIYVDERGKVLSYKMTSQCASGSGQFLENIARYLGVAVEEIGSLSQQSKDPEK
                     VSSICAVLAETDVINMVSRGIATPDILKGIHLSMASRIVKLLKVTGIKTGTALLTGGL
                     ALDSGLLAAIQEEMVNEKVSVNAVSHPDSIYAGAIGAALWGAFRHEKLAELQSRAAA"
BASE COUNT          480 a         1072 c         1077 g          523 t
ORIGIN      
        1 cgctggcgtc gaaggggctc gacacgctga aggcgggcga cctggtcagc attcgtcttc
       61 cgggcgcagg cggctacggc gatccgcggc aacgcgaccg cgcgctcgtg caggccgacc
      121 tgcgcgacga caagatcagc cgcgagtcgg ccgtgcgcga ttacggcctc gatgcaaccg
      181 aaccctgaat ccatgctgcg ctatcacacg gttcccgtca cgccgtttca gcagaacagc
      241 tcgatcgtct ggtgcgaccg gacgatggcg ggcgcagtga tcgatcccgg cggcgaaatc
      301 gcgcgattgc gcgctgccgc cgatcgattg ggcgtgcgcc tcgagcagat cctgctcacg
      361 catgcgcaca tcgaccacgc gggcggcgcc ggcgcgctcg cgcgcgagct cgcagtgccg
      421 atcatcggcc cccatccagg agatcagttc tggatcgatg cgctggcgca gcagtcgcag
      481 atgttcggcc tgccggccgc cgagacgttc acgccgagcc gctggctcac cgacggcgac
      541 acggtgcgcg tcggtaactg cgaactcgcg gtgcgccact gccccggaca cacgccgggg
      601 cacgtggtgt ttcatagtgt cgacacgcag cgcgcattcg tcggcgacgt gctgttcgcc
      661 ggctccatcg gccgcacgga tcttcccggc ggtgactacg acaccctgat cgccagcatc
      721 gtcgagcgcc tgtggccgat gggcgacgac acggtcttca tccccggcca cggccccgaa
      781 agcacgttcg gacgcgagcg gcgcagcaat ccgttcgtgg gcgggacgtg acgcggcgcg
      841 cacgcgcggg ccggtcgatt ccggttgatt acggaaatct ccgtaattac ggcaggcggc
      901 gtaatatgcc gtaatgagcg cgatcccgta ccggagctgg tacttccccc gcccggcgct
      961 ggcacagcgc atcctggcgc ttttgcttga gggtgcgggc gatccggtcg cgctggtcgg
     1021 ggcgcgccgc atcggcaaga cgtcgcatct gctgttcgaa ctgatgccgg cggcgcagaa
     1081 gcggggcctg ctgcccgtct acatcgacat gttccagcag cgcgccgatc cggtggcggc
     1141 gatcaactac gcgctgcagg aggcgatcga cgaggtcgag gtgccgcggt cgagggtcgg
     1201 tcggcgcttg agcacggcgg tgaagcggat cggcgccgga tcgttctcgc tcgacctggg
     1261 cgaagaaccc gcgcgccggc gccctgcgga tccgctgctg ctgatcgact ggctgctgaa
     1321 gacactggtg cgcaccgcgc ggaagcccgt gctgctgatc ttcgacgaag cgcaggaact
     1381 ggcgacgctg gccgacagcg acaacaccgt gtcggcaatc cgctcggcga tcaccaagag
     1441 ccggaacaac gtgcgcgtcg tattcaccgg ctcgtcgcag gaaagactgc tggcgctctt
     1501 cgcgcgcgcg cgcgccgcgc tgtacgaggg cgcgagcacg atgccgttcc cgcatctcgg
     1561 cgacgacttc atcgcgttcg tcgcgcagcg gtgccgggag ctcttccgca agcgcgtgtc
     1621 ggcggccgag ctgacggaag cctttgcgcg tctgcatcac cagccgcgcg cgctgatcga
     1681 cctcgtgctc gcatacgcgg cgggcgacca ggattcgctg gccgccgtcc tgaacgcgcg
     1741 catcgaggcg cagctgacgg gcgcggatta cagcgcgcag tgggcggcgc tcaagccgct
     1801 gcagcagcgc atctgcctgc gcgtgctgcg cggcgatgac gtcacctcgg tggcggcgcg
     1861 ccgcgaatac gcggcgggaa ccgacaagcc ggaaatcgcg cccggaaccg tcaacgatgc
     1921 gctgcgcgcg ctggtcgcgt cgcacgtgct gcacaagccg agcggcgcgc gcggcgcgta
     1981 tcgcatcgac gatccgctgt ttgcggagtg gctgcggcgc gagagcgcga gcctctccgg
     2041 acgcgggagt cgctgagggc gaccctcagc cgtgcagtgc ctggtacttc gccagcagct
     2101 cgtccttcga ttcctggaag tccgggttcg gcacgatgca gtcggccgga cacaccagca
     2161 tgcactgcgg ctcgtcctcg gcgccgacgc attcggtgca cttcagcgcg tcgatcacgt
     2221 agatcgggtc gccgacggaa atcgcttcgt tggggcaggg cggcttgcac gcgtcgcagg
     2281 cggtgcagtc ctgggtgatc atcaacgcca ttgcagttct cctgctacgc ggcagcgcgg
     2341 ctctgcagct ctgccagctt ctcgtgccgg aacgcacccc acagcgcggc gccgatcgcg
     2401 ccggcgtaga tcgagtccgg gtggctcacg gcgttcacgc tgactttctc gttgaccatt
     2461 tcctcctgga tcgcggcgag cagcccgctg tcgagcgcga gcccgccggt cagcagcgcc
     2521 gtcccggtct tgatgccggt gactttcagc agcttgacga tgcgcgaggc catcgacagg
     2581 tggatgccct tcaggatgtc gggcgtggcg atgccgcggc tcaccatgtt gatcacgtcg
     2641 gtctcggcga gcaccgcgca gattgagctc accttctccg ggtccttcga ctgctgcgac
     2701 agcgagccga tctcctcgac cgccacaccg aggtagcgcg cgatgttctc caggaactgc
     2761 cccgagcccg acgcgcactg gctcgtcatc ttgtacgaca gcaccttgcc gcgttcgtcg
     2821 acgtagatcg cgcggccgtt cagcgcgccg atatcgacca cggcgctgat gcccgggtgc
     2881 aggaacacgc cgccgcgcgc atgggtggtc atcgaataga agtggccggt ggcgaacttg
     2941 acgttctcgc cctcaccggt ggtggcgatg tagtcgatgt cgttggcggc gagcctggcc
     3001 tcggcgagta cgccgtcgag gccttcctgt gccagtgtca tcggatcgcg ccggcggatg
     3061 cgttcacagc gcttggcgat ccactgctgc tggccgccct cgacgcggaa cagcacgctc
     3121 ttgaccgcgc ccgagccaat atcaatgccg at
//
LOCUS       DBNHBY010000202         5133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1229823, whole genome shotgun sequence.
ACCESSION   DBNHBY010000202 DBNHBY010000000
VERSION     DBNHBY010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1229823"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="AC16HB_04895"
     CDS             complement(<1..125)
                     /locus_tag="AC16HB_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR5606162.1"
                     /translation="MFRKILIANRGEIACRVATTARRLGLRTVAVYSDADAGARHV"
     gene            complement(134..751)
                     /locus_tag="AC16HB_04900"
     CDS             complement(134..751)
                     /locus_tag="AC16HB_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR5606163.1"
                     /translation="MDKLDLAQLMALAATLGFASGIRLYAVLLIVGLVGHFDLAPLPA
                     GLSILAHPWVLAASGFMVAVEFLADKIPAVDSLWDAMHTFIRIPAGAALAAAVFGGDQ
                     VANQAAWATIAAILGGTLAATSHFTKAGARAAVNTSPEPFSNIAVSTAEDIAVGGLLW
                     LAIAHPFVAAAIVVALVALAVWLLPRIFRFIIGVFRRVFGVVKST"
     gene            complement(724..1359)
                     /locus_tag="AC16HB_04905"
     CDS             complement(724..1359)
                     /locus_tag="AC16HB_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606164.1"
                     /translation="MAERLRLTRTFGTPGWQAWHADGDGFVLAALGVCLLKPGASLAL
                     RGLWRLARIWRDARAIDPAALRIALCLQRAVQVSGVRLRVEVALPARRHARSKTYAYA
                     LRGQRRDAGLPAVEDGSAWTVLALAASDRRAFAAMAADLRDAPRGTRLTLDIAARASR
                     APDTLRADFPLRADALIDGAGWFPLLRATRIDVAGALAQGEGRWTSSTSHS"
     gene            complement(1413..2246)
                     /locus_tag="AC16HB_04910"
     CDS             complement(1413..2246)
                     /locus_tag="AC16HB_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5606165.1"
                     /translation="MSTDLLEVSVAHRVAVVWMAREKVRNAFNEAMIAELNATFTQLG
                     ADAGVRAIVLAGKGPAFCAGADLDWMRRMSGYTLEENRADARALAEMLRTIYLCPKPV
                     LARVHGDAYAGGIGLVAACDIAFAATTASFCLSETRLGLLPATISPYVIRAMGTNAAR
                     RYFLTAEKFDAAQAFRIGLVHDITPPDGLDAAINETLGALMLTSDHAVREAKKLVREV
                     AGRPIDDALVADTADRIAQIRASDDGREGVKSFLDKRKPRWVADYEAERAAAEGDDEE
                     M"
     gene            complement(2243..3073)
                     /locus_tag="AC16HB_04915"
     CDS             complement(2243..3073)
                     /locus_tag="AC16HB_04915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5606166.1"
                     /translation="MPMRWLCAATAVALIGAASAEPISLATVDGTRIEAHWFPAPASG
                     PRPAVVALHGCGGLYRRDGGTFDARYPEYIERLHRAGYHVLLPDSVGPRGSGPICRVK
                     SRDRSITVETRRMDAIAAVRWLAGRSDVDPQRIAVLGWSHGAMTTLSALNALREPHAQ
                     PLAGAVVFYPGCRALLERPYRLDVPLLMLLGERDDWTPPARCAQFAQRTRAAQPDADL
                     TLIVYPDSYHGFDSARPVRFLSGMPNGVDASGVHACGNPTARAQAQYEMDAFFARILK
                     "
     gene            complement(3073..3735)
                     /locus_tag="AC16HB_04920"
     CDS             complement(3073..3735)
                     /locus_tag="AC16HB_04920"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5606167.1"
                     /translation="MPTDELAELFDSNRAWSARRRADDPEFFARLARQQAPKYLWIGC
                     ADSRVPANEIVGLDPGELFVHRNIANVVAHTDFNCMSVLQFAIELLHVEHVIVVGHYQ
                     CSGIRCALGGGLQGVADHWLRHIEDVQRRHAAAFKAVAPRHHEDLLCELNVIEQVRNV
                     CRTNVVRGTWRAGRPLTIHGWVYGLADGLLQDLGWSPNASPDDLEAALAERAARLQAK
                     PR"
     gene            complement(3739..4890)
                     /locus_tag="AC16HB_04925"
     CDS             complement(3739..4890)
                     /locus_tag="AC16HB_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5606168.1"
                     /translation="MSASVFLRSEHVELQRQVARFVANEVEPHALAWDEAGCVPRAVL
                     RRMGALGWLGLMYPPEYGGAGADAITNAVFQEALSRATSGGFVVTVLVHTDMASPHLA
                     LAGTDAQKARWLPKIVRGECVTALAVTEPDAGSDVAAIRTRATRDGDAWVLNGTKMFI
                     TNGVHADLVFVAARTDLHAKGSRGISIFAIEKGTPGFRVGRALRKQGWLASDTAELAF
                     ENCRVPAANLIGDENAGFYAIMKNFQTERIALGSMAIGHCLTALELTFRHVNERRAFG
                     AALWDKQAVRQRLSMLAARVEAARQLLYHCAWLVDQKRDAVREVSMLKALTGELVNDV
                     MYDCQQFHGGAGYLRESPIERLVRDARVLAIGGGATEVMLEEVAKRFAG"
     gene            complement(4887..>5133)
                     /locus_tag="AC16HB_04930"
     CDS             complement(4887..>5133)
                     /locus_tag="AC16HB_04930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5606169.1"
                     /translation="DVAMWLAIMTMRGREERWVTSDMQTAFLRSGRAESIACRARVLR
                     LGRRSAFGAAECRGVDSGDLLAHHSVRYAKALGGEQV"
BASE COUNT          785 a         1849 c         1775 g          724 t
ORIGIN      
        1 acgtggcgcg cgccggcgtc ggcgtccgag tacaccgcga ccgtgcgcaa gccgagccgg
       61 cgcgcggtcg tcgcgacccg gcaggcaatc tctccccggt tggcgatcag gatcttgcgg
      121 aacactgaat tcttcaggtt gatttcacga cgccgaagac gcgccggaac acgccgatga
      181 tgaagcggaa gatcctcggc agcagccata ccgccagcgc aacgagcgcg acgacgatcg
      241 ccgcggcgac gaacggatgg gcgatcgcca gccacagcag gccgccgacc gcgatgtcct
      301 ccgcggtcga cacggcgatg ttggagaagg gctcgggcga cgtgttgacc gctgcgcgcg
      361 cgccggcctt ggtgaaatgg ctggtcgcgg ccagcgtgcc gccgaggatc gcggcgatcg
      421 tcgcccacgc tgcctgattc gcgacctggt cgccgccgaa cacggctgcc gcgagcgcgg
      481 cgccggccgg gatgcggatg aatgtgtgca tcgcgtccca cagcgagtcg acagccggaa
      541 tcttgtcggc gaggaactcg accgcaacca tgaagcccga cgccgcgagc acccacggat
      601 gcgcgaggat cgagagcccg gccggcagcg gcgcgagatc gaaatgaccg accagcccca
      661 cgatcagcag caccgcgtac aggcggatac cgctggcgaa gcccagcgtg gcagcgagcg
      721 ccatcaactg tgcgaggtcg agcttgtcca tctgccttct ccctgcgcca gcgcgccggc
      781 cacgtcgatc cgcgtggcgc ggagcagcgg gaaccagccc gcgccgtcga tcagcgcgtc
      841 ggcccgcagc ggaaagtccg cacgcagtgt atccggcgca cgactcgccc gcgcggcgat
      901 gtccagcgtg agccgcgtgc cgcgcggcgc gtcacgcagg tcggccgcca tcgccgcgaa
      961 cgcgcgccgg tcggatgctg ccagggcgag cacggtccat gcgctgccgt cctcgacggc
     1021 gggcaatccg gcgtcgcgcc gctgaccgcg cagcgcatac gcgtacgtct tcgagcgggc
     1081 gtgccgccgc gccggcagcg caacctcgac gcgcagccgg acgcccgaca cctgcacggc
     1141 gcgctgcagg cacagggcga tacgcagcgc ggcgggatcg atcgcccgcg cgtcgcgcca
     1201 aatccgggcg agccgccaca ggccgcgcag cgcgagcgag gcgccgggct tcagcaggca
     1261 tacgccgagc gccgccagca cgaagccgtc gccgtccgcg tgccaggcct gccagccggg
     1321 cgtgccgaac gtgcgcgtca gtcgcaggcg ttcagccatc gaaacctatc tccgagtctc
     1381 cggtggtcgc ggcggaaact aggagacagg tcttacattt cctcgtcatc gccttccgct
     1441 gccgcgcgct cggcctcgta atcggccacc cagcgcggct tgcgcttgtc gaggaagctc
     1501 ttcacgcctt cgcggccgtc gtcggacgcg cggatctgcg cgatgcggtc ggccgtgtcc
     1561 gccaccagcg catcgtcgat cggccggccg gcgacctcgc gcaccagctt cttcgcctcg
     1621 cgcaccgcgt gatcgctggt cagcatcagc gcgccgagcg tctcgttgat cgcggcgtcg
     1681 agtccatcgg gcggcgtgat gtcgtgcacg agcccgatgc ggaaggcctg cgccgcgtcg
     1741 aatttctcgg cggtcaggaa atagcggcgc gcggcattcg tccccatcgc gcggatcaca
     1801 tagggactga tcgtcgccgg cagcaggccg agccgggtct cgctcaggca gaagctcgcg
     1861 gtggtggcag cgaacgcgat gtcgcacgcg gccaccagcc cgatgccgcc cgcatacgcg
     1921 tcgccgtgca cgcgcgcgag caccggcttc ggacacaggt agatcgtgcg cagcatctcc
     1981 gcgagcgcgc gcgcgtcggc gcggttttcc tccagcgtgt agccgctcat gcgcctcatc
     2041 cagtccaggt ccgcgccggc gcagaacgcc ggtcccttgc cggcaagcac gatcgcgcgc
     2101 acgccggcat ccgcccccag ttgcgtgaag gtcgcgttca gctccgcgat catcgcttcg
     2161 ttgaacgcgt tgcgcacctt ctcgcgcgcc atccacacca ccgccacgcg gtgggcgacg
     2221 gagacttcca gcaggtcggt gctcatttca ggatcctcgc gaagaatgca tccatctcgt
     2281 attgcgcctg tgcgcgtgcc gtgggattgc cgcacgcgtg cacgccgctc gcatcgacgc
     2341 cgttgggcat gccggacagg aagcgtaccg ggcgcgcgct gtcgaagccg tgatagctgt
     2401 cgggatacac gatcaaggtc aggtccgcgt caggctgtgc tgcgcgcgtg cgctgcgcga
     2461 actgcgcgca gcgcgccggc ggcgtccagt cgtcgcgctc gccgagcagc atcagcagcg
     2521 gcacgtcgag ccggtacggc cgctcgagca gcgcgcgaca gccgggatag aagacgaccg
     2581 cgccggcgag cggctgcgcg tgcggctcgc gcagggcgtt cagcgcggac aaggtcgtca
     2641 tcgcgccatg cgaccagccg agcaccgcga tgcgctgcgg gtcgacatcg gaccgtcccg
     2701 cgagccagcg cacggccgcg atcgcgtcca ttcgccgcgt ctcgaccgtg atcgagcggt
     2761 cgcggctctt cacgcgacag atcgggcccg atccccgcgg gccgacactg tccggcagca
     2821 gcacgtggta gccggcgcgg tgcagccgct cgatgtactc cgggtagcgt gcgtcgaagg
     2881 tcccgccgtc gcggcgatac aacccgccgc agccgtgcaa cgcgacgacg gctggtcgcg
     2941 ggccggacgc gggcgcagga aaccagtgcg cctcgatcct ggtgccgtcg accgtggcga
     3001 gcgagatggg ctcggccgaa gcggcgccga tcagcgcaac cgcagttgcc gcgcacagcc
     3061 aacgcatcgg catcagcgcg gctttgcctg cagccgcgcg gcgcgctcgg cgagcgcggc
     3121 ctcgaggtcg tcgggagaag cgttcggcga ccagccgaga tcctgcagca gcccgtccgc
     3181 gaggccatag acccagccgt ggatcgtcag cggccgcccg gcgcgccacg tgccgcgcac
     3241 gacgttcgta cggcagacat tgcgcacctg ctcgatcacg ttcagctcgc acagcaggtc
     3301 ctcgtggtgg cgcggggcga ctgccttgaa ggcggccgcg tggcggcgct gcacgtcctc
     3361 gatgtgccgc agccaatggt cggccacgcc ctgcaaccca ccgccgagcg cgcagcggat
     3421 gccgctgcac tggtagtggc cgacgacgat cacgtgctcg acgtgcagca gttcgatcgc
     3481 gaactgcagc accgacatgc aattgaagtc cgtgtgcgcg accacgttgg cgatgttgcg
     3541 gtggacgaag agctcacccg gatcgaggcc gacgatctcg ttggcaggca cgcggctgtc
     3601 ggcgcagccg atccacaggt acttgggcgc ctgctggcgt gccagccgcg cgaagaattc
     3661 cggatcgtcg gcgcgccggc gcgcggacca ggcacgattg ctgtcaaaca gttcggcgag
     3721 ctcatcggtc ggcatcgtct accccgcaaa gcgcttggcc acttcctcca gcatcacttc
     3781 ggtcgcgccg ccgccgatcg caaggacgcg cgcgtcgcgc accagccgct cgatcggcga
     3841 ctcgcgcagg tagcccgcgc cgccgtggaa ctgctggcag tcgtacatca cgtcgttcac
     3901 gagctcgccg gtcagcgcct tgagcatcga cacttcgcgc accgcgtcgc gcttctgatc
     3961 gacgagccac gcgcagtgat acagcagctg gcgcgccgcc tcgacgcgcg cggccagcat
     4021 cgacaggcgc tgccgcacgg cctgcttgtc ccacagcgcg gcgccgaatg cgcggcgttc
     4081 gttgacatgg cggaacgtca gctcgagcgc ggtgaggcaa tgaccgatcg ccatgctgcc
     4141 caacgcgatc cgttcggtct ggaagttctt catgatcgcg tagaagccgg cgttctcgtc
     4201 gccgatcagg ttcgcggccg gcacgcggca gttctcgaac gcgagctccg cggtgtcgga
     4261 ggcgagccag ccctgtttgc gcagcgcgcg gccgacgcgg aagcccggtg ttcccttctc
     4321 gatcgcgaaa atcgagatgc cgcgcgagcc tttcgcgtgc aggtcggtgc gcgcggcgac
     4381 gaagaccagg tccgcgtgca cgccgttggt gatgaacatc ttggtgccgt tcagcaccca
     4441 cgcgtcgccg tcgcgtgtcg cgcgcgtgcg gatcgccgcg acgtcggagc cggcgtcggg
     4501 ctcggtgacc gccaacgcgg tgacgcactc gccgcggacg atcttcggca gccagcgcgc
     4561 cttctgcgca tcggtccccg cgagcgcgag gtgcgggctc gccatgtcgg tgtgcacgag
     4621 cacggtgacg acgaagccgc cgctggtggc gcgcgacagc gcctcctgga acacggcgtt
     4681 ggtgatcgca tcggcgccgg cgccgccgta ctccggcggg tacatgagcc ccagccagcc
     4741 gagcgcgccc atccggcgca gcactgcgcg cggcacgcag cccgcttcgt cccacgcgag
     4801 cgcgtgcggc tcgacttcgt ttgcgacgaa tcgcgcgacc tggcgctgca gttcgacgtg
     4861 ctcggagcgg aggaagaccg aggcgctcaa acctgctccc cgcccagagc ctttgcgtag
     4921 cgcaccgagt ggtgcgccag caggtcgccg gagtcgacgc cgcggcactc ggcggcgccg
     4981 aatgcgctgc ggcgcccgag ccgcaacacg cgcgccctgc aggcaatgct ctccgcgcgg
     5041 ccgctgcgca ggaacgcggt ctgcatgtcc gacgtgaccc agcgctcctc gcgtccgcgc
     5101 atcgtcatga tcgccagcca catcgcgacg tcg
//
LOCUS       DBNHBY010000203         2078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1635522, whole genome shotgun sequence.
ACCESSION   DBNHBY010000203 DBNHBY010000000
VERSION     DBNHBY010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1635522"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16HB_04935"
     CDS             complement(<1..570)
                     /locus_tag="AC16HB_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606170.1"
                     /translation="MDIFGIPSQALFGQLLIGLINGSFYALLSLGLAVIFGLLNIINF
                     THGAQYMLGAFCAYLLLNRLGIGYWPALVIAPIIVGATGIVIERLMLSKLYKLDHLYG
                     LLLTFGLALIIQGLFRNEYGSSGLPYTIPQQLTGGTNLGFMFLPNYRGWVIFASLVIC
                     LSTWYVIERTKLGAYLRAATENAQLVQAFG"
     gene            complement(632..1849)
                     /locus_tag="AC16HB_04940"
     CDS             complement(632..1849)
                     /locus_tag="AC16HB_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606171.1"
                     /translation="MKRKLLSTFVAAALAFGTAAQAQISDGVVKIGVLSDMSSLYSDI
                     GGPGSTLAAKLAVEDFQKTSKAGLKIEIVSADHQNKPDVGSNIARTWYDTDKVDVIVD
                     VPTSSVALAVSQVTCEKGKAFLVSGAASSDLTGKACSPNTIHWTYDTWMLANGTGGAI
                     VKTGGDSWFFLTADYAFGHALERDTEAVVLKSGGKVLGKVRHPLNAQDFSSFLLQAQS
                     SKAKIIGLANAGGDTINSIKQAAEFGIVKGGQNLAGLLVFLTDIKALGLPTAQGLIFT
                     ETFYWDMNDATRAFAQRFSKEAPRNNYPTMVQAGVYSAVLHYLKAIEAIKTDEGTKVV
                     EQMKKMPTDDPLFGKGTIRADGRKIHPAYLVEVKKPAESKGPWDLYKIRATIPAEQAF
                     RPIDQGDCPLVKK"
     gene            complement(1891..>2078)
                     /locus_tag="AC16HB_04945"
     CDS             complement(1891..>2078)
                     /locus_tag="AC16HB_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606172.1"
                     /translation="ARMIRMLREKGLTIVMVEQNFRFAAPLADRFYVMERGRIVEGFT
                     AAELSSKTELLHEYLGV"
BASE COUNT          373 a          662 c          656 g          387 t
ORIGIN      
        1 gccgaacgcc tgcaccagtt gcgcgttctc ggtcgccgcg cgcaggtacg cgccgagctt
       61 ggtgcgctcg atcacgtacc aggtcgacag gcagatcacg agcgaggcga agatcaccca
      121 gccccgatag ttggggagga acatgaaccc gaggttcgtg ccgccggtca gttgttgcgg
      181 aatcgtgtag ggcaggcccg acgatccgta ctcgttgcgg aacaggccct ggatgatcag
      241 cgccagtccg aaagtcagca gcaggccata caggtggtcg agcttgtaca gcttcgacag
      301 catcagccgc tcgatcacga tgccggtggc gccgacgatg atcggcgcga tcacgagcgc
      361 cggccagtag ccgatgccga gcctgttcag cagcagatag gcgcagaagg cacccagcat
      421 gtactgggcg ccgtgcgtga aattgatgat gttcagcagc ccgaagatca ccgccagccc
      481 gagcgacagc agcgcgtaga acgagccgtt gatcagcccg atcagcagtt gcccgaacag
      541 ggcctgggag gggatgccga aaatgtccat acggtcgggg atgtcggtgg gtgtaaccgg
      601 cggggccggc tacaccaccg actactcggg cctatttctt gaccagaggg cagtcgccct
      661 ggtcgatcgg gcggaacgcc tgctcggccg gaatcgtggc gcggatcttg tacaggtccc
      721 acggtccctt cgactcggcc ggtttcttga cttcgaccag ataggccgga tggatcttgc
      781 ggccgtcggc gcggatcgtg cccttgccga acagcgggtc gtcggtcggc atcttcttca
      841 tctgctcgac caccttcgtc ccttcgtcgg tcttgatcgc ctcgatcgct ttcaggtaat
      901 gcagcaccgc cgaatagacg ccggcctgga ccatcgtcgg gtagttgttg cgcggcgcct
      961 ctttcgagaa gcgctgcgcg aacgcgcgcg tggcatcgtt catgtcccag tagaaggtct
     1021 cggtgaaaat cagcccctgc gcggtcggca gcccgagcgc cttgatgtcg gtcaggaata
     1081 ccagcagccc ggccaggttc tgcccgccct tgacgatgcc gaactcggcc gcctgcttga
     1141 tcgagttgat cgtgtcgccg ccggcattgg ccagcccgat gatcttggcc ttcgacgact
     1201 gcgcctgcag caggaacgac gagaagtcct gtgcattcag cggatgccgc accttgccca
     1261 gcaccttgcc gccgctcttc aataccacgg cctcggtgtc gcgctcgagc gcatggccga
     1321 acgcgtagtc ggcggtcagg aagaaccacg agtcaccgcc ggtcttgacg atcgcgccgc
     1381 cggtgccgtt ggccagcatc cacgtgtcat aggtccagtg gatcgtgttc ggcgagcagg
     1441 ccttgccggt cagatcggac gacgccgcgc ccgacacgag gaatgccttg cccttctcgc
     1501 acgtgacctg gctgaccgcc agcgcgaccg aggaggtcgg tacgtcgacg atcacgtcca
     1561 ccttgtcggt gtcgtaccac gtgcgggcga tgttcgatcc gacgtcgggc ttgttctggt
     1621 ggtcggccga gacgatctcg atcttcaggc cggccttcga ggtcttctgg aagtcctcga
     1681 ccgccagctt tgccgcgagt gtcgaacccg gtccgccgat atcggaatac aggctcgaca
     1741 tgtcgctcaa cacgccgatc ttgaccaccc catcggaaat ctgcgcctgc gccgcggtgc
     1801 cgaacgcgag cgcggcagcg acgaaggtgg ataacagctt gcgtttcatg tgctctcctc
     1861 ttgcctacgt gccggaaacg gatgggccgg tcacacaccc aggtattcgt gcagcaattc
     1921 ggtcttcgat gaaagctctg cagcggtgaa accctcgacg atgcggccgc gctccatcac
     1981 gtagaagcga tcggccagcg gtgcggcaaa gcggaagttc tgctcgacca tcacgatcgt
     2041 aagacccttt tcccgcagca tgcggatcat gcgcgcca
//
LOCUS       DBNHBY010000204         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1365117, whole genome shotgun sequence.
ACCESSION   DBNHBY010000204 DBNHBY010000000
VERSION     DBNHBY010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1365117"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..778
                     /locus_tag="AC16HB_04950"
     CDS             164..778
                     /locus_tag="AC16HB_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR5606173.1"
                     /translation="MSDFTPTRSLAIALADERRTMREGYAFLDEKCLLLAGRMLAEVR
                     RWREWSRELRAAQEGAHAQLAAAIARHGLNGLQVYAAAVSMQPVTTEHNDLIGVTLLD
                     AHVAGEAPAPPPAVHPSPEAEACRRAFSALIAKMVEMAALSGNLARLYAEYRKTVRRV
                     RALQDVLLPELEGTLAEIETSLEELEQDEYGFLRRAVRHGEAAP"
     gene            928..1116
                     /locus_tag="AC16HB_04955"
     CDS             928..1116
                     /locus_tag="AC16HB_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR5606174.1"
                     /translation="MSVWTVLIVVALAATVYSFVCGVSTMAVDGEVRHMSGEKWMFLR
                     VGFQALTAALVLVALAME"
     gene            complement(1154..1618)
                     /locus_tag="AC16HB_04960"
     CDS             complement(1154..1618)
                     /locus_tag="AC16HB_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606175.1"
                     /translation="MGLLDLAGGLLQQYLNASPAQANAGAEQHFDQVAQQASPDLLSR
                     GLAAAFRSDQTPDFAQMAAQMFGNASPEQKQALLAQLQSVLGPAAGALAGGGAVAPST
                     VQSALEQAHANNPSIIDRVSGFYAQHPDLIKTLGGAALTVALAKMAEHARQG"
     gene            complement(1629..1877)
                     /locus_tag="AC16HB_04965"
     CDS             complement(1629..1877)
                     /locus_tag="AC16HB_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5606176.1"
                     /translation="MHIVWTILIGLVVGLLARFLMPGDDKMGLIMTAALGIAGSLVAT
                     YAGHLLGWYVAGQGAGFIASLIGAIVLLFVYHLVRKST"
     gene            complement(2054..2575)
                     /locus_tag="AC16HB_04970"
     CDS             complement(2054..2575)
                     /locus_tag="AC16HB_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HIR5606177.1"
                     /translation="MKRIHAAALAATLLATFFNCLAAAPAGAPAGSTGLCNDGSYYSG
                     PEKKGACRGHKGVKDWWGEASAAKGEKKAEAKAEAKVPAKAAPAPAAKPSAIATTPAA
                     APAAAPKAEPPKPAAAAAPGGGAGKVWVNESTKVYHCAGDRWYGKTKQGEYMSEADAK
                     AKGFKPDHGKACQ"
BASE COUNT          415 a          909 c          894 g          437 t
ORIGIN      
        1 accgagttcg agcagcggct ggtgaatcag gccggcgcgc gcacgctggt gcagagcatg
       61 gaagcgggct gggaactgct gcgcgcgctg ccggtcgcgg agctcacgcg gctgtccgac
      121 gcgcagatcg ccgagcacct gtcgcgcagc gcgctgtaag ccaatgagcg acttcacgcc
      181 gacgcgcagc cttgcgatcg cgctcgcgga cgagcgccgc acgatgcgcg agggctacgc
      241 gttcctcgac gagaagtgcc tgctgctcgc gggccgcatg ctggccgagg tccggcgctg
      301 gcgcgaatgg tcacgcgagt tgcgggccgc gcaggaaggt gcacacgcgc aactggccgc
      361 cgcgatcgcg cgccatgggc tgaacggcct gcaggtgtac gcggcggcgg tgtcgatgca
      421 gccggtcacc accgagcaca acgacctgat cggcgtcacg ctgctcgatg cgcatgtggc
      481 cggcgaagcc cccgcgccgc cgcctgccgt gcatccgtcg cccgaggcgg aggcgtgccg
      541 gcgcgcgttc agcgcgctga tcgcaaagat ggtcgaaatg gccgctctct ccggcaacct
      601 cgcgcggctg tacgccgaat accgcaagac cgtgcgccgc gtgcgcgcgc tgcaggatgt
      661 gctgctgccc gaactggagg gcacgctggc cgagatcgaa acgagcctgg aagaactcga
      721 acaggacgaa tacggcttcc tgcgccgcgc ggtacggcat ggcgaagcag ccccctagtc
      781 gacgacgcag tgcagcacgc cggcacccat cgtcgagacg ggatgcgcca tccgccgcaa
      841 actgatctcg atcaaggcac cgccgattcg atcggcgtag caaatgcggt gcggtcaact
      901 cgtcgttgcc gcgcccggga ggatgccatg agtgtgtgga ccgtcttgat cgtcgtggcc
      961 ctggcggcca ccgtttactc gttcgtctgc ggcgtttcga cgatggccgt cgacggtgaa
     1021 gtgcgccaca tgtcgggcga gaagtggatg ttcctgcgcg tcggcttcca ggcgctcacc
     1081 gccgcgctgg tgctcgtcgc gctggcgatg gagtagagcc gcggcgcccg gcgcgccgca
     1141 cgtcagcgcg cgatcagccc tgccgcgcgt gctcggccat cttggccagg gcgacggtca
     1201 gggcagcgcc gcccagcgtt ttgatcaggt cgggatgctg cgcgtagaag ccgctgaccc
     1261 tgtcgatgat cgacggattg ttcgcgtgcg cctgttccag cgccgactga accgtcgatg
     1321 gcgcgaccgc gccgccgccc gccaacgcgc cggccgcagg ccccagcacc gactgtagtt
     1381 gcgccagcag cgcctgcttc tgttcgggcg aggcattgcc gaacatctgc gcggccattt
     1441 gcgcgaagtc cggcgtctgg tcggagcgga aagccgccgc caggccccgg ctcagcaggt
     1501 ctggcgaggc ctgctgcgcg acctggtcga agtgctgttc cgcccccgca ttcgcctgag
     1561 cgggagaggc attcagatac tgctgcaaca gtccgcccgc gagatcgagg aggcccatcg
     1621 ttgctccttc aggtgctctt gcgaaccagg tgatagacga acagcagcac gatggcaccg
     1681 atcagcgaag cgatgaatcc ggcgccctga ccggccacat accagccgag caagtggccc
     1741 gcataagtcg cgaccagcga ccccgcgatg ccgagcgcgg ccgtcatgat gaggcccatc
     1801 ttgtcgtcac ccggcatcag aaagcgcgcg agcagtccga ccaccagccc gatcaaaatc
     1861 gtccagacga tatgcatact cgacctccca tgtcgttgtt gtcgttcgat ccgcgcgcag
     1921 cgtaggcgcg ctacccgcgc gcggcaatcc cgggacagcc gcaccgatgc cttggtcgcg
     1981 ggatggccgg ggatcagtca acgcacactg gcgcatcaaa cacgtgaagc gccaatgccg
     2041 ccacgagcgg agcctactgg caggccttgc cgtgatcggg cttgaacccc ttggcctttg
     2101 cgtcggcctc gctcatgtac tcgccctgct tggtcttgcc gtaccagcga tcgcctgcgc
     2161 agtggtacac cttggtcgat tcgttgaccc agaccttgcc ggcaccgcct cccggcgcag
     2221 ccgccgccgc gggtttcgga ggctcggctt ttggcgccgc ggcgggcgcc gccgcgggag
     2281 tggttgcgat cgcgctcggc ttggcggcag gtgcgggggc ggccttggcc ggcactttcg
     2341 cttcagcctt ggcttccgcc ttcttttcgc ccttcgcggc cgacgcttca ccccaccagt
     2401 ccttcacgcc cttgtggccg cgacaggcgc ctttcttctc tggaccggag tagtaagacc
     2461 cgtcgttgca cagccccgtc gatccggccg gtgcgccggc gggtgctgca gccagacaat
     2521 tgaagaacgt tgccagcagc gtagctgcca gcgcggcagc atgaatgcgc ttcatcgaat
     2581 gctctccctg cttattggaa tgggtgcgtg cagcggcacg ccggcggatc gtaacagttg
     2641 ccccattgcg gcggc
//
LOCUS       DBNHBY010000205         4597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1365123, whole genome shotgun sequence.
ACCESSION   DBNHBY010000205 DBNHBY010000000
VERSION     DBNHBY010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1365123"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /gene="purD"
                     /locus_tag="AC16HB_04975"
     CDS             <1..1195
                     /gene="purD"
                     /locus_tag="AC16HB_04975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5606178.1"
                     /translation="HARLANLAVTNIDALADFAQKNEIALTVVGPEAPLAAGAVDAFR
                     ARGLRVFGPTRAAAQLESSKDFAKAFMKRHGIPTADYETFADPISAHAYVERKGAPIV
                     IKADGLAAGKGVVVAQTLGEAHAAIDAMLLDHRMGAAGARVVIEDFLAGEEASFIVMC
                     DGRNVLALASSQDHKRLRDGDQGPNTGGMGAYSPAPAITPTLHARVMREIIVPTIQGM
                     AADGIVYTGFLYAGLMIDDAGRARTLEFNCRLGDPETQPIMLRLKSDLVELLEHAMEG
                     TLDRAEAEWDRRSALGVVIAAAGYPEAPRKGEVIHSLPADAEDCVTFHAGTELTADGA
                     LVTSGGRVLCVTALGDSVRVAQRRAYEAAAQVKFAGSQYRTDIGHRAIGRGPGAYNAA
                     RGGAA"
     gene            1192..3180
                     /locus_tag="AC16HB_04980"
     CDS             1192..3180
                     /locus_tag="AC16HB_04980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5606179.1"
                     /translation="MTYTVAQHIQLHRVSEAAAAPDGTWLAVVVQRLDRDGAKYVSDI
                     WRVPTDGGAATQLTRGDCKDSAPCFRHDGSLGFLSNRQPNELEPDDEAEKRMQVWLLP
                     AGGGEPKQLTDEPLGVESFRFARNADRLVLFAPVLAGVPYEQQREVATKRAKEGPSAR
                     RFSRQPVRHWDRWLHENPDLPCTHVVAYDASGGNRTDLTPEARREFAIEPALDVSADG
                     RRVAVTRQTPGKDRELDTTLLVIDLETRTQRVLGEADNVNFDAPLFSPDGSRIATTRS
                     TRSPKLAVRPVATLVDVTSGEMRQPAAHWDRWPSLARWSHDGKQLIATADDEGHTPVF
                     TIDAANGTVERITAWAAGGAHSNLEVLRDGRIAGIRSTLLDAPECFVVEASPVSVPKS
                     LARLSGFAPATEWAEVESMTTPSTDGTPIHAFLVKPRGASGKLPLLLWIHGGPIGMDV
                     DGWHWRWNPLLAVAAGYAVALPNPRGSTGFGQDFVQGIWGNVWGDQCYKDLMAVTDAL
                     VARSDIDAARSMAMGGSFGGYMTNWIGTQTDRFKCLITHASIVTMAQFTGTTDHPAWW
                     YLEMGGENPYEDMARFDRFAPIRHIRNWKTPVLIIHGELDYRCPVNEGLNLFEALQYH
                     GVDSELLLFPDENHWILKPKNIVAWYEAVLGFIARHLK"
     gene            3177..4097
                     /gene="hemF"
                     /locus_tag="AC16HB_04985"
     CDS             3177..4097
                     /gene="hemF"
                     /locus_tag="AC16HB_04985"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR5606180.1"
                     /translation="MTEFVDSQSVREYLLGLQQRIVDRFVQLDGRPFMRDVWEKPHDA
                     PLTGGGLTRIIENGNLLERGGVGFSHVNGTRLPPSASAGRPELAGRGFEAMGVSLVLH
                     PHNPYVPTVHMNVRMFVARREGEPDVYWFGGGMDLTPYYGFEEDARHFHATCRTALEP
                     FGADLHPRFKRWCDEYFFLQHRNEPRGVGGIFFDDFHALGFERGFALLRSVGNAFLDA
                     YVPIVERRFSHPYGERERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPP
                     VVKWRYDWQPAPGTPEARLYTDFLPARDWV"
     gene            4097..>4597
                     /gene="nadD"
                     /locus_tag="AC16HB_04990"
     CDS             4097..>4597
                     /gene="nadD"
                     /locus_tag="AC16HB_04990"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR5606181.1"
                     /translation="MPAPIGLLGGSFDPIHLGHLQLARDTLRQLRLAEVRFVPAGQPW
                     QKHVATAAGHRARMVELAIRGEDHFMLDVHEIERAGPTYTIDTLRTLRATLGAAARLV
                     LILGGDQMQRLDTWHEWQHLLDYAHLAVARRNDAVLTLSWPLQEFYNSHWAPPDAIVA
                     RPAGSVV"
BASE COUNT          755 a         1568 c         1596 g          678 t
ORIGIN      
        1 gcacgcgcgg ctggccaatc tggcggttac caacatcgac gcgctcgccg acttcgcgca
       61 gaagaacgag atcgcgctga cggtggttgg ccccgaggcg ccgctggcgg cgggcgcggt
      121 cgacgcattt cgcgcgcgcg ggctgcgggt gttcgggccg acccgcgcgg cagcacaact
      181 ggagagctcg aaggacttcg ccaaggcgtt catgaagcgc cacggcattc cgaccgcgga
      241 ctacgagacc ttcgccgatc cgatctccgc gcacgcctat gtcgaacgca agggcgcgcc
      301 catcgtgatc aaggcggacg gcctggcggc cggcaagggc gtggtcgtcg cacagacgct
      361 cggcgaggcg cacgcggcca tcgacgcgat gctgctcgac caccgcatgg gcgcggccgg
      421 agcacgggtc gtcatcgagg acttcctcgc gggcgaggaa gcgagcttca tcgtgatgtg
      481 cgacggccgc aacgtgctgg cgctggcgag cagccaggat cacaagcgcc tgcgcgacgg
      541 cgaccagggg cccaataccg gtggcatggg cgcgtattcg ccggcgccgg cgatcacgcc
      601 gacgctgcac gcgcgggtga tgcgcgagat catcgtgccg acgatccagg gcatggcggc
      661 cgacggcatt gtctacacgg gcttcctgta cgcgggtctg atgatcgacg atgccgggcg
      721 ggcgcgcacg ctcgagttca actgccggct gggcgacccc gaaacgcagc cgatcatgct
      781 gcggctcaaa tccgacctgg tcgagttgct ggagcacgcg atggaaggca cgctcgaccg
      841 cgccgaagcg gaatgggacc gccgcagcgc gctgggcgtg gtgatcgccg cggcgggcta
      901 tccggaggcg ccgcgcaagg gcgaagtgat ccacagtctg cccgccgacg ccgaagactg
      961 cgtgaccttt catgccggca ccgagctcac cgccgacggc gcgctcgtca ccagcggcgg
     1021 ccgcgtgctg tgcgtgaccg cgctcggcga cagcgtgcgc gtggctcagc ggcgtgcgta
     1081 cgaggccgcc gcgcaggtga agttcgccgg cagccagtac cgcaccgaca tcggccaccg
     1141 cgcgatcggc cgcggaccgg gcgcatacaa cgccgcgcgc gggggtgcag cgtgacctac
     1201 accgtcgcgc agcacatcca actgcatcgc gtcagcgagg cagccgccgc gccggacggc
     1261 acgtggctcg cagtggtcgt gcagcggctc gatcgcgacg gcgccaagta cgtgagcgac
     1321 atctggcgcg tgccgaccga cggcggtgcg gccacgcagc tgacgcgcgg cgactgcaag
     1381 gacagcgcgc cctgctttcg acacgacggc tcgctcggct tcctgtcgaa ccggcagccg
     1441 aacgagctcg agcccgacga cgaggccgag aagcggatgc aggtctggct gctgccggcc
     1501 ggcggcggcg agccgaaaca gctgaccgac gagccattgg gggtcgagag cttccgcttc
     1561 gcacgcaacg ccgaccggct ggttctcttc gcgccggtgc tcgccggcgt gccgtacgag
     1621 cagcaacgcg aggtcgcgac gaagcgcgcc aaggagggtc cgagcgcgcg ccgcttttcg
     1681 cgccaacccg tgcggcattg ggaccgctgg ctgcacgaga accccgacct gccctgcacg
     1741 cacgtggtcg catacgatgc gtcgggcggc aaccgcaccg acctgacgcc ggaggcgcga
     1801 cgcgaattcg cgatagagcc cgcgctcgac gtgtcggccg acggcagacg cgtcgcggtg
     1861 acgcggcaga cgccgggcaa ggaccgagag ctcgacacga cgctgctggt gatcgacctc
     1921 gaaacgagaa cgcagcgcgt gctgggcgaa gcggacaacg tgaacttcga cgcgccgctg
     1981 ttttctcccg acggcagccg catcgccacc acgcgcagca cgcgcagtcc caagctcgcg
     2041 gtgcggccgg tggcgacact cgtcgatgtc acgagcggcg aaatgcggca gccggcggca
     2101 cactgggacc gctggccgag cctggcgcgc tggagccatg acggcaagca gctcatcgcc
     2161 accgccgacg acgagggtca cacgccggtc tttacgatcg acgccgccaa cggcaccgtc
     2221 gagcgcatca ccgcgtgggc cgcgggcggc gcgcattcga acctcgaggt gctgcgcgac
     2281 ggccgcatcg cgggcatccg ttcgacactg ctcgacgcgc ccgagtgttt cgtcgtcgag
     2341 gcgagcccgg tgtcggtgcc gaaatcgctg gcgcgtctgt cgggatttgc gccggcgacg
     2401 gaatgggccg aggtcgagag catgacgacg ccgtcgacgg acggcacgcc gatccacgcg
     2461 ttcctggtca agcctagagg cgccagcggc aaactgccgc tgctgctgtg gatccacggc
     2521 ggcccgatcg gaatggacgt cgacggctgg cactggcgct ggaatccgct gctggcggtg
     2581 gcagcgggct acgcggtcgc gctgccgaac ccgcgcggct cgaccggttt cggccaggac
     2641 ttcgtccagg gcatctgggg caacgtgtgg ggcgaccagt gctacaagga cctgatggcg
     2701 gtcaccgatg cgctggtcgc gcgttccgac atcgacgccg cgcgctcgat ggcaatgggc
     2761 ggatcgttcg gcggctacat gaccaactgg atcggcacgc agaccgatcg cttcaaatgc
     2821 ctgatcacgc acgccagcat cgtcacgatg gcgcagttca ccggcacgac cgatcatccg
     2881 gcgtggtggt acctggagat gggcggcgaa aacccgtacg aagacatggc gcgcttcgat
     2941 cgcttcgccc cgatccgcca catacgcaac tggaagacgc ccgtgctgat catccacggc
     3001 gaactcgact accgctgtcc ggtcaacgaa ggactcaatc tgttcgaggc gctgcagtac
     3061 cacggcgtcg acagcgaact gctgctcttc ccggacgaga accactggat cctgaagccg
     3121 aagaacatcg tcgcgtggta tgaggcggtg ctcggcttca tcgcgcgtca tctcaaatga
     3181 ccgaattcgt cgactcgcaa tcggtacgcg agtacctgct cggcctgcag cagcgcatcg
     3241 tcgatagatt cgtgcaactc gacggccgac ctttcatgcg cgacgtgtgg gagaagccgc
     3301 atgatgcacc gctgaccggc ggcggtctga cgcgcatcat cgaaaacggc aacctgctag
     3361 agcgcggcgg cgtgggtttc tcgcacgtga acggtacgcg gctgccgccg tcggcttcgg
     3421 cgggtcgccc cgaacttgcg ggccgcggct tcgaggcgat gggcgtgtcg ctggtcttgc
     3481 atccgcacaa tccgtacgtg ccgaccgtgc acatgaacgt gcgcatgttc gtcgcgcgca
     3541 gggaaggcga gcccgacgtg tactggttcg gcggaggcat ggacctgacg ccgtactacg
     3601 gcttcgagga ggacgcgcgc catttccacg cgacgtgcag gactgcgctc gagccgttcg
     3661 gcgccgatct gcatccgcgc ttcaagcggt ggtgcgacga gtacttcttc ctgcagcacc
     3721 gcaacgaacc gcgcggtgtc ggcggcatct tcttcgacga cttccacgcg ctcggtttcg
     3781 agcgcggctt cgcgctgctg cgctcggtcg gcaacgcctt cctcgacgcg tacgtgccga
     3841 tcgtcgaacg acgcttcagc cacccgtatg gcgaacgcga gcgcgatttc caggcctacc
     3901 ggcgcggccg ctacgtcgag ttcaatctcg tgtacgaccg cggcacgctg ttcgggttgc
     3961 agtcgggcgg ccgcaccgaa tcgatcctga tgtcgctgcc gccggtcgtg aagtggcgct
     4021 acgactggca gcccgcaccg ggcacgcccg aggcgcgcct gtacaccgac tttctgccgg
     4081 cacgcgactg ggtgtgatgc ctgcacccat cggcctgctt ggcggcagct tcgatccgat
     4141 tcacctcggt catctgcaac tggcgcgcga tacgctgcgg cagctgcggc tcgccgaagt
     4201 gcgcttcgtg cccgccggcc agccgtggca gaagcacgtc gcgaccgctg ccgggcatcg
     4261 cgcgcgcatg gtcgaactgg cgatccgtgg ggaagaccac ttcatgctcg acgtgcacga
     4321 gatcgaacgc gcgggcccga cctacacgat cgacacgctg cgcacgctgc gggccacgct
     4381 cggcgcggcc gcgcggctgg tgctgatcct cggcggcgac cagatgcagc gcctcgacac
     4441 gtggcacgaa tggcagcatc tgctcgacta cgcgcacctg gcagtcgcgc gccggaacga
     4501 cgcggtgctc acgctttcgt ggccgctgca ggagttctac aactcgcact gggcgccgcc
     4561 cgacgcgatc gtggcgcgcc cggccggatc ggtcgtc
//
LOCUS       DBNHBY010000206         4124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1770368, whole genome shotgun sequence.
ACCESSION   DBNHBY010000206 DBNHBY010000000
VERSION     DBNHBY010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1770368"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC16HB_04995"
     CDS             complement(<1..370)
                     /locus_tag="AC16HB_04995"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106430 - dihydroorotate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR5606182.1"
                     /translation="MLYEFARPLLFALDPEVAHDLAFANLRRAHAVGLTRCLSPRVAD
                     DPVDVMGLSFPNPIGLAAGLDKNGAHVDALARLGFGFVEVGTVTPRPQPGNPKPRLFR
                     LPAAEALINRFGFNNDGLERF"
     gene            complement(401..1162)
                     /locus_tag="AC16HB_05000"
     CDS             complement(401..1162)
                     /locus_tag="AC16HB_05000"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR5606183.1"
                     /translation="MANLKELPFSALQFYATAPYPCSYLEERQARSQVATPAHLINAD
                     VYSELVRMGFRRSGIFTYRPHCDGCRACIPVRIPVARFSPNRSQRRAWRGHRRLVARV
                     VGLAFDPEHYELYLRYQATRHAGGGMDNDSREQYSQFLLQSKVNTRLVEFREPTPCND
                     PNERGRLLLVSIIDILNDGLSSVYTFFDPDIERASFGTYNVLWQIEQCRQLGLPYLYL
                     GYWIEASAKMAYKANFEPTEMLLQGRWIQRPKPSR"
     gene            complement(1171..1893)
                     /gene="aat"
                     /locus_tag="AC16HB_05005"
     CDS             complement(1171..1893)
                     /gene="aat"
                     /locus_tag="AC16HB_05005"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR5606184.1"
                     /translation="MIPWLEPGAAFPPVDRSLVSPNGLLAATAEVTEARLLDAYRHGI
                     FPWYSEGDPVLWWSPDPRMVLFTDELRVSRSLRKRVRAFAGEGANEVVVDRDFVGTMQ
                     ACAQPRRDQDGTWITDDVIASYAALHRRGLAHSIEVWCKGVCVGGLYGVALGRMFFGE
                     SMFSRQTDASKVALVALVELLLREGCPVIDCQQKTSHLASLGAREIRRAEFCTLVARM
                     VDAPSMNWPHYAGRPINELLTK"
     gene            complement(1890..2465)
                     /locus_tag="AC16HB_05010"
     CDS             complement(1890..2465)
                     /locus_tag="AC16HB_05010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5606185.1"
                     /translation="MVTRSPTRFCASCGAAIVRRVPSGDNRERDCCDQCGAIHYINPR
                     PVVGTIPLWEDRILLCRRAIDPRYGKWTLPAGFMEVGETTAGGAQRETTEEAGARVEL
                     GPVFSILDVPHVEQVHIFFRARLLDLDFAPGTESLEVRLFEESAIPWEELAFRTVSMT
                     LRLFFADRARGAFGVHMQAVPPPPALRNTGT"
     gene            complement(2558..2634)
                     /locus_tag="AC16HB_05015"
     tRNA            complement(2558..2634)
                     /locus_tag="AC16HB_05015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2598..2600),aa:Met,seq:cat)
     gene            3007..3645
                     /locus_tag="AC16HB_05020"
     CDS             3007..3645
                     /locus_tag="AC16HB_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HIR5606186.1"
                     /translation="MWVRTVSLKVKALVSIATLCCAGLVVSGCGSVGSFPPAPASAAT
                     PEYRYLIGPLDTVNIIVWRNPELSMSVPVRPDGRITTPLVDDLPALGRSSTDLARDLE
                     KALAKVIRDPVVTVIVTGFQGPFSEQIRIVGEAAKPQAIPYRQNMTLLDVMIVVGGLT
                     DFADGNGAVLVRGKEGGKQYSVRLRDLVKRGDISANVDVKPGDVLIIPQSWF"
     gene            3702..>4124
                     /locus_tag="AC16HB_05025"
     CDS             3702..>4124
                     /locus_tag="AC16HB_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIR5606187.1"
                     /translation="MDELLNQAITFLRGTWHRRWIGLGVAWIVAIIGAVVLLRIPDRY
                     EASARIYVDTDSVLKPLMAGLAVQPNVEQRVSMLARTLISRPNMERLMRSSDLDLLAT
                     TARERDAVIDGLIESITIMNDGRGPNLYLVSYQDVHPER"
BASE COUNT          752 a         1270 c         1349 g          753 t
ORIGIN      
        1 cgaagcgctc caggccgtcg ttgttgaagc cgaagcggtt gatcagcgct tcggcggcag
       61 gcaggcgaaa cagtcgcggc tttggattgc ccggctgggg tcgcggcgtc actgttccga
      121 cttcgacgaa accgaacccg aggcgcgcga gcgcgtcgac gtgcgcgccg ttcttgtcga
      181 gccccgccgc cagaccgatc gggttgggaa aactcaatcc catcacgtcg accggatcgt
      241 cggcaacgcg cggcgacagg cagcgcgtga ggccgacggc gtgtgcacgc cgcaggttcg
      301 cgaacgccag gtcgtgtgca acctccgggt cgagggcaaa gagcagtggg cgcgcgaatt
      361 cgtacagcac gcctcgattc tacggtcggg cgaccgtgga ctaccgcgac ggtttgggcc
      421 gctgaatcca ccggccttgc agcagcatct cggtcggttc gaagttggcc ttgtaggcca
      481 tcttggcgct cgcttcgatc cagtagccga ggtacaggta agggaggcca agctggcggc
      541 actgttcgat ctgccacaac acgttgtagg tgccgaagct cgcgcgctcg atgtccgggt
      601 cgaagaatgt gtagacggac gacagaccgt cgttgaggat gtcgatgatc gacaccagta
      661 gcaaccggcc gcgctcgttt gggtcgttac agggtgtggg ttcgcggaac tcaaccagcc
      721 gcgtattgac cttgctctgc aggaggaact gcgagtactg ctcgcggctg tcgttgtcca
      781 tgccgccgcc cgcatgacgc gtcgcctgat accgcaggta gagttcgtaa tgttccgggt
      841 cgaatgcaag tccgacgacg cgcgcgacga ggcggcggtg cccccgccat gcgcgtcgct
      901 ggctgcggtt cggcgagaac ctggcgaccg gaatgcggac cgggatacag gcacggcaac
      961 cgtcgcagtg cgggcgatag gtgaagatcc cgctgcgccg gaaccccatc cgcaccagtt
     1021 cgctgtagac gtcggcattg atcaggtgcg cgggcgtggc gacctgcgag cgggcctggc
     1081 gttcctcgag atagctgcag ggatagggcg ccgtcgcgta gaactgcagc gccgagaaag
     1141 gcagttcctt gaggttcgcc acgccggaat ctacttcgtc agcagttcgt tgatcgggcg
     1201 gcccgcgtag tgcggccagt tcatcgatgg tgcatcgacc attcttgcca cgagcgtgca
     1261 gaactctgcc ctgcggatct cgcgcgcgcc gagcgaggcg aggtgcgaag tcttctgctg
     1321 gcagtctatc accgggcatc cctctcgaag cagcaactcg accagggcaa ccagcgccac
     1381 cttggacgcg tctgtctggc gactgaacat cgattcgccg aagaacatgc gaccgagcgc
     1441 cacgccatat agcccgccga cacagacgcc cttgcaccag acttcgatcg aatgcgccaa
     1501 cccgcgccga tgcagcgcgg cgtacgaagc gatcacgtcg tccgtgatcc acgtgccgtc
     1561 ctggtcgcgg cgcggctgcg cgcacgcctg cattgtgccg acgaaatcgc gatcgaccac
     1621 gacctcgttt gcgccttcgc ctgcgaacgc gcgcacgcgc ttgcgcagcg aacggctgac
     1681 gcgtagttca tcagtgaaca ggaccatgcg gggatccggc gaccaccaca gcaccggatc
     1741 gccttccgag taccagggaa agatgccatg ccggtaggcg tccagcaggc gggcttccgt
     1801 gacctcggct gttgcggcga gcagcccgtt cggtgagaca agtgaccgat caaccggcgg
     1861 aaacgcggcg ccgggctcga gccacggaat catgtccccg tgtttcggag cgcgggaggc
     1921 ggcgggacag cttgcatgtg tacgccgaac gcgccgcgcg cccggtccgc gaagaagagt
     1981 ctgagcgtca tcgagaccgt cctgaaggcg agttcttccc acggaatcgc cgactcctcg
     2041 aacaacctga cttcgagact ttctgtacct ggcgcgaaat ccaggtcgag cagccgcgca
     2101 cgaaagaaga tgtgcacctg ctcgacatgc gggacatcga gaatcgaaaa caccggaccc
     2161 agttcgacgc gcgcgccggc ttcctcggtc gtttcgcgct gcgcgccgcc ggcagtcgtc
     2221 tcgccgactt ccatgaagcc ggcgggcagc gtccacttgc cgtaccgcgg atcgatcgct
     2281 cgacggcaca acagaatccg gtcttcccag agcgggatcg tgccgaccac gggccgcggg
     2341 ttgatgtagt gaatcgcacc gcattgatcg cagcaatcgc gttcgcggtt gtctcccgac
     2401 ggcacgcgac gcacaatggc ggcgccgcaa ctggcgcaga agcgagtggg actgcgtgtg
     2461 accacgaagc cagtctagca accggggccg ccaattgacg tgcggtcagt cgcggccggg
     2521 aattgaaaag ggcctcgctc ggaggccctg cattgtctgg ttgcggggga aggatttgaa
     2581 ccttcgacct tcgggttatg agcccgacga gctgccagac tgctccaccc cgcgtcgagg
     2641 aggcagagta taccccatcg gactctgagc attgctttcc gccgtcgcgc cggcgatgcg
     2701 aggcgtcgcc gagtcgtccg gatcgaaatt ctttgctccc tcgctctgcc aatcgacgga
     2761 tttcaactaa cctcgcgatt gcgtagggtt ggctgcatga cggtgtcagg caagacggat
     2821 agtcttctgc gatggccgag ccgtcggtgc agtgcagatg agcgaaatgc gacagaatgt
     2881 ccgcgcatcg gggccgggat gccatcggtg ttgctgcgcc acgggcgcat tcggctgtgt
     2941 aatagactgg caagacaagt cggcgcgcgg cgttttccct ttgtaatcag ctacctcgga
     3001 ggagtcatgt gggtgcgcac ggtctcactt aaggtcaagg cgctcgtctc gatcgcgacc
     3061 ctatgctgcg caggtctggt cgtgtccggc tgcgggtccg ttggcagctt ccccccagcg
     3121 ccggcgagtg ccgcgactcc ggagtaccgg tacctcattg gacccctcga taccgtcaac
     3181 atcatcgtct ggcgcaaccc cgagttgtcg atgtcggttc cagtcaggcc ggatggccgc
     3241 ataacgacac cgctcgtcga tgaccttccg gcgctcggtc gcagttcgac tgacctcgcg
     3301 cgcgatctgg aaaaggcgct tgcgaaggtg atccgcgatc cggtggtcac ggtgatcgtg
     3361 acgggattcc aggggccgtt cagcgaacaa atccgcatcg tcggcgaggc ggcgaagccg
     3421 caggcaattc cgtaccgtca gaacatgacc ttgctcgacg tcatgatcgt tgtcggcggt
     3481 ctgaccgatt tcgcggacgg caacggcgcc gtgttggtgc gcggcaagga gggtggaaag
     3541 cagtactccg tccggctgcg cgacctcgtc aagcggggcg atatctccgc aaacgtcgat
     3601 gtgaagccgg gcgatgtcct gatcattccg cagagttggt tctagtggct cgcattggac
     3661 ggttcgctgc atacccctca gggattccga gacgctgaac gatggacgag ttgcttaacc
     3721 aggcaataac gttcttgcgt ggcacctggc atcggcgctg gatcggcttg ggtgtcgcct
     3781 ggattgtcgc gatcatcgga gccgtagtgc tgctgcggat accggatcgc tacgaggcga
     3841 gtgcccgtat ctacgtcgat accgattcgg tgctcaaacc gctgatggct ggtctggccg
     3901 tgcagccgaa cgttgaacag cgggtttcca tgctcgcgcg aacgttgatc agccggccga
     3961 acatggaacg gctcatgcgc agttcggacc tcgatctgct cgcaacaacg gcccgagaac
     4021 gagatgcggt gatcgacggc cttatcgaga gtatcacgat catgaacgac gggcgcgggc
     4081 cgaatctcta cctcgtgtcg tatcaggatg ttcatcccga gcgc
//
LOCUS       DBNHBY010000207         2127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1094972, whole genome shotgun sequence.
ACCESSION   DBNHBY010000207 DBNHBY010000000
VERSION     DBNHBY010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1094972"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..827
                     /locus_tag="AC16HB_05030"
     CDS             228..827
                     /locus_tag="AC16HB_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5606188.1"
                     /translation="MKPIEFYFDFSSPYSYLASEKIEAIAAKYGRTVAYKPTLLGVAF
                     KAAETRPLVDYPLKGDYSRRDFARSARFHGVKLNMPEKFPISTVNAARALLWLQGTGS
                     AKAVSFVHKTFRTYWVDNRDINDPAVLADIADELGIDKAALLAAIQDPAIKDKLKAVV
                     EESVARGVFGAPFFFVDGEPFWGHDRLPQIERWLERGPF"
     gene            841..1167
                     /locus_tag="AC16HB_05035"
     CDS             841..1167
                     /locus_tag="AC16HB_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606189.1"
                     /translation="MSYFYLAIAIVAEVIGTSALKATEGFTRAVPTVIVAIGYGVAFY
                     FLALALRHIPVGVAYAIWSGVGIALITLIGWIVFKQALDAAALAGILLIVLGVVVIRV
                     YSQAAP"
     gene            1164..1643
                     /locus_tag="AC16HB_05040"
     CDS             1164..1643
                     /locus_tag="AC16HB_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606190.1"
                     /translation="MTRPPTIDERMRDVFEVAPGAHDPLADLAATAGASIEEHLHAQS
                     RRRRAWAAYPWLLLAIVAGVTGTSALSVSAAFTRALPVAVMALAYLVCFLALTHALKT
                     IPVGIAYAIWSGVGIVLIALIGWRVFGQQLNVGELAGIALILIGVVVIQLFSRTKVK"
     gene            1727..>2127
                     /locus_tag="AC16HB_05045"
     CDS             1727..>2127
                     /locus_tag="AC16HB_05045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5606191.1"
                     /translation="MMPTTTRVPHRVFGTSTSGNCHKVRLALDLLKLPYRWHEVDIMK
                     GETRTPEFLAMNPNGRVPLLQIDDHTWLAESNAILCYLADGTHLWPGDRLQRARALQW
                     MFFEQYSHEPYIAVARFILKFLKQPDHPRLP"
BASE COUNT          333 a          725 c          702 g          367 t
ORIGIN      
        1 gcggcacgag atcggcgcgg cgcttcagtt gcacgtcgat gtcggcgtag ccctgcgcga
       61 ccaggttgcg gtccgcgatc aaccggttga atacccagac gccgtaggcg accagcgccg
      121 ccggcagcag ccaccaccag accacgattc gcctccccga gtgcggcggc atcgtacgca
      181 cgagccggct gcggcacaat cgagctgtcg accgctctgg aaagtccatg aaaccgatcg
      241 agttctactt cgatttctcg tcgccctact cctacctcgc cagcgaaaag atcgaggcga
      301 tcgccgccaa gtacggccgc acggtcgcgt acaagcccac gctgctcggc gtcgccttca
      361 aggcggcgga aacgcggccg ctggtcgact atccgctgaa gggcgattac agccggcgcg
      421 actttgcgcg cagcgcgcgc tttcacggcg tgaagctcaa catgccggag aaattcccga
      481 tctcgaccgt caatgcggcg cgcgcgctgc tgtggctgca gggcaccggg tcggccaagg
      541 cggtgtcgtt cgtgcacaag accttccgca cctactgggt cgacaaccgc gacatcaacg
      601 atccggcggt gctggccgac atcgccgacg aactgggcat cgacaaggcc gcgctgctgg
      661 ccgcgatcca ggacccggcg atcaaggaca agctcaaggc ggtggtcgaa gagtcggtcg
      721 cgcgcggcgt gttcggcgcg ccgttcttct tcgtcgacgg cgaaccgttc tggggccacg
      781 accgcctgcc gcagatcgag cgctggctgg agcggggtcc gttctgacgc ggccgccggc
      841 gtgtcgtact tctacctcgc gatcgcgatc gttgccgaag tgatcggtac ctcggcgctg
      901 aaggccaccg aaggattcac gcgcgccgtg cccacggtca tcgtcgcgat cggctatggg
      961 gttgccttct acttcctggc gctggcgctg cggcacatcc ccgtcggggt cgcgtacgcg
     1021 atctggtccg gtgtgggcat cgcgctgatc acgctgatcg gctggatcgt gttcaagcag
     1081 gcgctcgacg cggccgcgct ggccggcatc ctgctgatcg tgctcggcgt cgtggtgatc
     1141 cgggtgtatt cgcaggcggc gccatgacac ggccgccgac gatcgacgag cggatgcgcg
     1201 acgtgttcga ggtcgcgccc ggcgcacacg atccgctcgc cgatctcgcc gcgaccgcgg
     1261 gcgcgagcat cgaagagcat ctgcatgcgc agtcgcggcg ccggcgcgcg tgggccgcgt
     1321 atccgtggct gctgctggcg atcgtcgccg gcgtgaccgg cacctcggca ctgtcggtga
     1381 gcgcggcgtt cacccgcgcg ctcccggtcg ccgtgatggc gctcgcctac ctggtgtgct
     1441 ttctcgcgct cacccacgcg ctgaagacga ttccggtcgg aatcgcctac gcgatctggt
     1501 ccggcgtcgg catcgtgttg atcgcgctga tcggctggcg cgtgttcggc cagcaactga
     1561 acgtcggtga actcgccggc atcgcgctga tcctgatcgg cgtggtcgtg attcagctgt
     1621 tctcccgcac gaaggtgaaa taggatgaaa cagcccccac gctcgctcag ctcgctgccc
     1681 cccgaggggg cgcgtcagta ccttcggacg gccgggcggt actgacatga tgcccaccac
     1741 cacccgcgtg ccgcaccgcg tgttcggcac ctcgaccagc ggcaattgcc acaaggtgcg
     1801 gctggcgctc gatctgctga agctgccgta tcgctggcac gaagtcgaca tcatgaaggg
     1861 cgagacgcgc acgcccgagt tcctggcgat gaaccccaac ggcagggtgc cgctgctgca
     1921 gatcgacgat cacacgtggc ttgccgaatc gaacgcgatc ctctgttatc tcgccgacgg
     1981 cacgcacctc tggccgggcg accggctgca gcgggcgcgc gcgctgcaat ggatgttctt
     2041 cgagcagtat tcgcacgagc cgtacatcgc ggtggcgcgc ttcatcctga agttcctgaa
     2101 gcagcccgac catccgcgtc tgcccga
//
LOCUS       DBNHBY010000208         1625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1432324, whole genome shotgun sequence.
ACCESSION   DBNHBY010000208 DBNHBY010000000
VERSION     DBNHBY010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1432324"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="AC16HB_05050"
     CDS             <1..1005
                     /locus_tag="AC16HB_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5606192.1"
                     /translation="GGYVFGDYMSNTFLSFGAHSSAIYAALVIVALTAVNIAGLRQSA
                     RTQNVMTSLLVLGMLGVAVASILAPVPAPPAAAAPTTGVPPLLATALLFVLFTFGGWN
                     EAAYISAEVKGGRRTILAVLVLALALITAVYLAFIWALVSGLGFDALKASKVPGADVM
                     RAAFGPLGEKFLGGVAALAALTTINATMIVGARTNYALGRDWPIVAFMSAWDGGRDAP
                     VKAYLVQAAIALALVMLAAFEKSGVATMVEFTAPVFWFFFMLTGIALIVLRFRRPNAA
                     RPFLVPGYPILPIVFIGTCGWLFYSSVTYAQSQKAVSVALYVMGAGLVAWLIARQKKA
                     "
     gene            1002..1229
                     /locus_tag="AC16HB_05055"
     CDS             1002..1229
                     /locus_tag="AC16HB_05055"
                     /inference="COORDINATES: protein motif:HMM:NF049803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7661 family protein"
                     /protein_id="HIR5606193.1"
                     /translation="MIRSVRLDVFGETMVAEFDATGWTLYRVGNEGKRSDTGLVIPSF
                     ITVEELDQYLFDLLHEAAKPSHPTIRRLPER"
     gene            1226..>1625
                     /locus_tag="AC16HB_05060"
     CDS             1226..>1625
                     /locus_tag="AC16HB_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR5606194.1"
                     /translation="MKLVGLLLAAGTGQRFDPTGARLKLLEPAPLGTHAGAPIAIAAA
                     RALRATLADVRAVVRPAANDLLQQLHALLSAEGCRLVVCERADEGMGASLACGVRAAA
                     DADGWIVALADMPAVRATTVAAVCAALERGA"
BASE COUNT          213 a          550 c          574 g          288 t
ORIGIN      
        1 ggcggctatg tgttcgggga ttacatgtcg aacacatttc tgtcgttcgg tgcgcattca
       61 tcggcgatct acgcggcgct ggtgatcgtc gcgctcaccg cggtcaacat tgccggcctg
      121 cgccagagcg cccgcacgca gaacgtcatg acgagcctgc tggtgctcgg catgctcggc
      181 gtggcggtcg ccagcattct ggcgcccgtc cccgcgccgc cggccgccgc ggcgccgacg
      241 accggcgtgc cgccgctgct cgcgactgcg ctgctgttcg tgctgttcac cttcggtggc
      301 tggaacgagg cagcctacat ctccgccgag gtcaagggcg gccgccgcac gatcctcgcg
      361 gtgctggtgc tcgcgctcgc gctgatcacc gcggtgtatc tcgcgttcat ctgggcgctg
      421 gtcagcggac tgggcttcga tgcgctgaag gcgtcgaagg tgcccggcgc cgatgtgatg
      481 cgtgccgcgt tcggcccgct cggcgagaag ttcctcggcg gcgtcgcggc gctcgcggcg
      541 ctgaccacga tcaacgccac catgatcgtc ggcgcgcgca ccaactacgc gctcggccgc
      601 gactggccga tcgtcgcgtt catgagcgca tgggacggcg gccgcgacgc gccggtcaag
      661 gcttacctgg tgcaggccgc gatcgcgctc gcgctcgtca tgctggcggc gttcgaaaag
      721 agcggggtcg ccacgatggt cgaattcacc gcacccgtgt tctggttctt tttcatgctg
      781 accggcatcg cgctgatcgt gctgcgattt cggcggccga acgccgcgcg cccgttcctg
      841 gtgcccggct acccgatcct gccgatcgtg ttcatcggga cctgcggctg gctgttctac
      901 tcgagcgtca cctatgcgca gtcgcagaag gcggtcagcg ttgcgctcta cgtgatgggc
      961 gcggggctcg tcgcctggct gatcgcgcgg cagaagaagg cgtgattcgg agcgtccgcc
     1021 tcgacgtgtt cggcgaaacg atggtcgccg aattcgatgc gacgggctgg acgctgtatc
     1081 gcgtcggcaa cgaaggcaag cgcagcgata ccggactcgt gattccgtcg ttcatcacgg
     1141 tcgaggaact ggaccagtat ctgttcgacc tgctgcacga ggcggcgaag ccgtcgcacc
     1201 cgaccattcg ccggctgccg gagcgatgaa actcgtcgga ttgctgctcg ctgccggcac
     1261 cggccagcgc ttcgacccga ccggtgcacg gctgaaattg ctcgagccgg cgccgctggg
     1321 cactcatgcc ggcgcgccga tcgcgatcgc agccgcgcgc gccctgcggg cgacgctcgc
     1381 cgacgttcgc gccgtcgtgc ggccggccgc gaacgacttg ctgcagcaac tgcacgctct
     1441 gctcagcgcc gagggttgcc ggctggtcgt gtgcgagcgt gccgacgaag gaatgggcgc
     1501 gtcgctcgcg tgcggcgtgc gcgctgcggc cgacgccgac ggctggatcg tcgcgctggc
     1561 cgacatgcct gcggtgcgcg caacgacggt cgcggccgtg tgcgcggcac tggaacgcgg
     1621 cgcgg
//
LOCUS       DBNHBY010000209        13108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1229933, whole genome shotgun sequence.
ACCESSION   DBNHBY010000209 DBNHBY010000000
VERSION     DBNHBY010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1229933"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="AC16HB_05065"
     CDS             <1..1183
                     /locus_tag="AC16HB_05065"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR5606195.1"
                     /translation="EAVDFLRYYAAQAERHLAEQPLPGPVGEANRLRFAGRGVFVCIS
                     PWNFPLAIFAGQIAAALVAGNAVIAKPAEQTPLISFDAVRLMHAAGVPRAVLQLVPGR
                     GESVGAALTSDPRVTGVAFTGSTATARAINRALAARDAPIGVLIAETGGQNAMIVDST
                     ALPEQVSDAVVASAFRSCGQRCSALRVLCLQSEIADSMLAMIMGAMRELRMGDPADPR
                     TDVGPVIDAEQLAALQRHRDWLRANATRIHECELPAGLDGHYFAPIAYEIGSIAQLTQ
                     EHFGPILHVVRFDKLKLDALLNEINATGYGLTCGLHTRLDARIDQVAARIRAGNLYVN
                     RNMIGAVVGVQPFGGEGLSGTGPKAGGPHYLLRFCTERTLTINTAAAGGNVELAAGLA
                     G"
     gene            complement(1180..2766)
                     /locus_tag="AC16HB_05070"
     CDS             complement(1180..2766)
                     /locus_tag="AC16HB_05070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5606196.1"
                     /translation="MDCDVLVIGAGHNGLVCACYLAAAGLKVRIVERRSVVGGAAVTE
                     EFVPGFRNSSASYTVSLLNPRVIRDLKLVERGLRIVERPFGNFLPLAEGTFLKFGGSA
                     TEAAAEVAKFSVRDSSALPAYFARCERVASLLRGWMLRAPPNLGGPLLVRDWRDALAA
                     ARLLVQLGRMPRVSQRDLIDLLTKSAGHWLDATFESEPLKGVLGWDAVVGNYASPYAP
                     GSAYLLLHYALGEIHGRRGQWGHPIGGMGRITELMAGEARARGVQIEVEAPVERVLVE
                     RGRAGGVALSGGREIRARAVAANVNPKLLYQRLVARADLPEDFAQSIDRYRCGSGSFR
                     INVALSELPDFACLPGTQAQAHHASGIVFAPSLACMDRAWRDAQVDGFARTPIVEMLI
                     PSVLDDSLAPRGAHVASLFAQQFKPDADWERLKDAAVRAIFDVVESYCPNFRRSLLGW
                     RAWSPLDLEREFGLVGGDILHGQMSLDQLFSLRPVHGYARYRGPIAGLYHCGAGAHPC
                     GGVSGAPGHNAAREIIRDLR"
     gene            complement(2785..3261)
                     /gene="bfr"
                     /locus_tag="AC16HB_05075"
     CDS             complement(2785..3261)
                     /gene="bfr"
                     /locus_tag="AC16HB_05075"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR5606197.1"
                     /translation="MQGDKAVVAQLNAQLKNEISAVNQYFLHARMLRQWGYGKMGKKE
                     YDESIDEMKHADRLIERILLLGGLPNLQDLAKLQIGQDVPEVLKGDLALERGSQQTLK
                     GAIAKCESAHDFVSRQILVDILDDTEEHIDYIETQLALIDQVGQQNYLQSQMDAGS"
     gene            complement(3301..3513)
                     /locus_tag="AC16HB_05080"
     CDS             complement(3301..3513)
                     /locus_tag="AC16HB_05080"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5606198.1"
                     /translation="MYVCICNAVSDQQVKAAVAAGARSLGELSLQLGVGAGCGCCRDA
                     AQEYILSAGRCGGDCATCSRGGASAA"
     gene            complement(3522..4598)
                     /locus_tag="AC16HB_05085"
     CDS             complement(3522..4598)
                     /locus_tag="AC16HB_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5606199.1"
                     /translation="MNAGDRRAERWLEGALSAVCALGLAVGAALPWSSHAQSGGDKVL
                     NLYSARHYQTDEALYENFTRQTGIGINRIELGDEPLLQRLKSEGANSPADVVLLVDAA
                     RLWRAQIDGLFQPIKSKVLDERIPAELRSNDGSWFGFSTRARVIVYDKSRLQPTDVDT
                     YEKLADPKLKGLVCTRSGSHPYMLSLIGAMVERSGEAATQAWAKGVVANMARPPRGGD
                     TDQIRAVASGECGVALTNTYYWVRLVRSSDAKDKDVVAKVGFVWPNQATSGTHVNVSG
                     GGVARHAPHRDNAIRFLEYLSSKEAQAYFAEGNNEWPVVKGAENINPALAALGTFKHE
                     SVPISSIGKGQIAAQRLLDRVGYR"
     gene            complement(4701..5225)
                     /locus_tag="AC16HB_05090"
     CDS             complement(4701..5225)
                     /locus_tag="AC16HB_05090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR5606200.1"
                     /translation="MALAVAVPSRADEAVAEPSAGAEIVMRALSFLGVPYRLGGEDPA
                     RGFDCSGLVRHVVRVALGFDLPRRSDAIGTVGRSVAPEESLAPGDLLFFNTLGRPFSH
                     VAVYIGEGRFVHAPARGGRVRIESMQFAYWRARFSGARRIAGGSPDVSATPGSVGAGT
                     QAFVDAVSVADNRP"
     gene            5465..6646
                     /locus_tag="AC16HB_05095"
     CDS             5465..6646
                     /locus_tag="AC16HB_05095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR5606201.1"
                     /translation="MSDDANFKNTAPLPPRARLLIADDSRIVRASITQHIRDRFDIRE
                     VTDGEAAWQAILLDASIRVLISDLTMPKIDGFELLSRIRSSKVQRIRELPVVVITGAD
                     EEAERERAAALGATDFIQKGVNAAELISRLEVLVKLSTTRETLSETQATLESARTIDP
                     DTELHNLVFFDRQVDKLISYSRRNLSDVALICIRVELSMPQSDSWEGETESRVKLVGR
                     TLAASIRLEDLGTRSDRYEFCVATQTNGLSGVLRFSARLRKVLENVDAAGPGVEVWTC
                     IGVATLSEDLRRGAEELRAIAQRRAQQAQANRSRRIVLGPAEGAASGSADARPDEVSM
                     DVSLALAVIRAGRANEVVPHLPRILAQIEPLLKLVRQQREIQTNPSEGARDDQLQGVP
                     R"
     gene            6618..8513
                     /locus_tag="AC16HB_05100"
     CDS             6618..8513
                     /locus_tag="AC16HB_05100"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HIR5606202.1"
                     /translation="MTSYKEFHAKSISDPEGFWGEQARLIDWQTPFSKVLDYSKPPFA
                     KWFVGGRTNLCHNAIDRHLQARGDQAALIYVSSETNQDKTYTFRQLHREVNRFAGILK
                     GLGVGHGDRVLIYMPMVPEAVFAMLACTRIGAIHSVVFGGFASVSLATRIDDAQPKVI
                     VSADAGSRAGKVVPYKGLLDEAITLAKFPPRNVVMINRGLAEMSLVAGRDLDYAKLRE
                     KHMNDEVPCEWLESSEPSYVLYTSGTTGKPKGVQRDTGGYAVALTSSMQCIFCGNAGE
                     TYFSTSDIGWVVGHSYIVYGPLLAGMATVLYEGTPIRPDGGVWWSIVEKYKVTSMFSA
                     PTAIRVLKKQDPAFLRKYDLSSLRLLFLAGEPLDEPTARWIADGIGKPIIDNFWQTET
                     GWPILALCRGVEALPPKFGSPGLPVYGYDVRLYNEMTGAEIGDNQKGVVGINGPLPPG
                     CMSTVWGDDQRFVDTYWKTIPNRMVYSTFDWGIRGADGYFLILGRTDDVINVAGHRLG
                     TREIEESISSHPNIAEVAVVGVADQLKGQVAMAFAVLKDHGKVATPELRRAHEGEMMK
                     VVDDQLGAVARPARVHFVSVLPKTRSGKLLRRSIQAICEGRDPGDLTTIEDPTALEQV
                     RTALKSG"
     gene            8524..9306
                     /locus_tag="AC16HB_05105"
     CDS             8524..9306
                     /locus_tag="AC16HB_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5606203.1"
                     /translation="MIDFTLETFSLSYFLASFLLPPILLLITAIIGAARLWRQPYSWP
                     ARVLLALSLAGMLILSLPVVAFSLFNPLEVRPLDSAQARAAQAIVIVAGGRARGAIEW
                     GGDTVNGFSLTRLRYGAHLARRTGLPVLVTGGGGTAERAPEAVLMQRALQGEFGIAPR
                     WVESASRTTAENARFSAAMLRTAGITRVLLVTHSFHMRRAAAQFERAGLQVIPAPTGF
                     QGLRDFEWLHLVPSPDALRLSHVALREWLALARDRLISHFGS"
     gene            9318..9722
                     /locus_tag="AC16HB_05110"
     CDS             9318..9722
                     /locus_tag="AC16HB_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR5606204.1"
                     /translation="MQSRKYLTLDDVQRIGAAATAEAQKNKWAVTIAIVDDGGHLLWL
                     QRLDGAATASVYIGQEKARSAALGRRETKRYEDMINQGRTAFLSAPLAGMLEGGVPIV
                     AEGHVIGAVGVSGVQSSEDVQIAKAGIAALGV"
     gene            complement(9724..11022)
                     /locus_tag="AC16HB_05115"
     CDS             complement(9724..11022)
                     /locus_tag="AC16HB_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606205.1"
                     /translation="MPLFDPQALNPGVTRREVFAWSCYDFANSGYTTVVLTAVFNAYF
                     VSVVARGADWATLAWTLALSASYVVGMVLMPPLGAYADLHAKKKLMLLVVTVGCIVGT
                     VALAFVGPGDLALAAAALVVSNFCFSAGVTLNSAFLPELARPEALGKVSGWGWSFGYL
                     GGLLALGLGLGYVQWAQARGQTATQFVPVTMLITAAVFALAAAPMFALVRERAVPRAG
                     VSVGNFLAESTQRMWHTLKNIGAHRDFGWLLVCGFFYQGGIAVVIALAAIYAQQAMHF
                     TMAQTMLLILLVNITAAIGAFLFGYVQDALGHRATLAITIAGWIAMVLIAALSTGTTG
                     FWIAANIAGLCMGSSQSAGRALVGVFAPTARLAEFYALWNAAQWLSGVVGPMTYGLVT
                     WITDNDHRLAILVTGLYFVVGLALLAPIDVERGRRAGQIE"
     gene            11064..11531
                     /locus_tag="AC16HB_05120"
     CDS             11064..11531
                     /locus_tag="AC16HB_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5606206.1"
                     /translation="MKTIDRIDRQILEVLQQDGRLTNQALSERVALSPRACLERVRRL
                     EKAGYISGYMALVEPRKLGGLLTIVVEVSLRDQSAATHARFEARMRAADEVIECFLVS
                     GACDYVLRLACRSLDHYRDLTNAWTDDPTLGVERIASKPELQTVKPFRGYPIP"
     gene            complement(11500..12186)
                     /locus_tag="AC16HB_05125"
     CDS             complement(11500..12186)
                     /locus_tag="AC16HB_05125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5606207.1"
                     /translation="MTTYFMTPTGTHPALASLRRAGFHAQRIDQVLVFYDEPHRHYHD
                     REHIGEMLDAALGLGVTLTPAQSLALLFHDAVYVPGAARGTNEAMSAQLLRAFAHGLA
                     LDCIERAAQIVLDTTEHATRSLEAELVLDLDLLRLAAPPLHFERYSRAVLAEQRALIR
                     LEDDEAAYRYFCRLRRPFFERLLARASIYRTPLASKAFEAAARANLLMAVSTAGDAAA
                     VRESDIRETA"
     gene            12412..>13108
                     /locus_tag="AC16HB_05130"
     CDS             12412..>13108
                     /locus_tag="AC16HB_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5606208.1"
                     /translation="MDIRRTLATEAIAREAAVAAPNYHPLPVVLQSGRGEWLTDVDGK
                     RYLDMMSAYSAVSHGHAHPRLVRVLIEQAQRVAVTSRAYHTDTLAPFLEKLTQLTGLD
                     RALPANGGAESVETAIKAARRWGYRARGIASDRAEIVVARGNFHGRSTTIVSFSTEAG
                     YRDGFGPFTPGFRHFDFGDAESLGAAINERTCAVLIEPIQGEAGIVVPPDGFLRAARR
                     LCDERKVLLILDEI"
BASE COUNT         2255 a         4493 c         4306 g         2054 t
ORIGIN      
        1 cgaggccgtc gattttctgc gctactacgc cgcgcaggcc gagcgccacc tggccgaaca
       61 gccgctgccc ggaccggtcg gcgaggcaaa ccgattgcgg ttcgccggac gaggcgtgtt
      121 cgtctgcatc agcccgtgga actttccgct cgcgatcttc gccgggcaga tcgcggccgc
      181 actcgtcgcc ggcaacgcgg tgatcgccaa acccgccgag cagacgccgc tcatttcctt
      241 cgatgcggtg cgcctgatgc acgcggcggg cgttccgcgc gctgttctgc aactcgtgcc
      301 gggacgcggc gaaagtgtcg gcgcggcgct cacctccgat ccgcgcgtga ccggcgtcgc
      361 gttcaccggg tcgaccgcga ccgcacgcgc catcaaccgc gccctggccg cgcgcgacgc
      421 gccgatcggc gtgctgatcg ccgaaacggg cgggcagaat gcaatgatcg tcgactctac
      481 ggcgctgccc gagcaggtga gcgacgcggt cgtagcgagc gccttccgct cctgcggcca
      541 gcgctgcagc gcgctgcggg tgctgtgcct gcagagcgaa atcgccgact cgatgctggc
      601 gatgatcatg ggcgcgatgc gcgagttgcg catgggcgat cccgccgatc cgcgcaccga
      661 cgtcggcccc gtgatcgacg cggagcaact cgccgccctg caacgacacc gcgactggct
      721 gcgcgcgaac gcgacgcgga ttcacgaatg cgaactgccg gccggactcg acggccacta
      781 cttcgcaccg attgcctacg agatcgggtc gatcgcgcaa ctgacgcagg agcacttcgg
      841 cccgatcctg cacgtggtcc ggttcgacaa gctgaagctc gacgcgttgc tcaacgagat
      901 caacgccacc ggatacgggc tgacctgcgg gctgcacacg cggctcgacg cgcgcatcga
      961 tcaggtggca gcgcgcatcc gcgccggaaa tctttacgta aaccgcaaca tgatcggcgc
     1021 cgtcgtcggc gtgcagccct ttggcggcga agggctgtcg ggaacgggac cgaaggccgg
     1081 cgggccgcac tacctgctgc gcttctgcac cgaacgcacg ctgacaatca acaccgcggc
     1141 ggccggcggc aatgtcgaac tcgcggcggg gctcgccggc taacgcagat cgcggatgat
     1201 ctcgcgcgcc gcgttgtgtc ccggtgcgcc gctgacgccg ccgcaggggt gcgcgccggc
     1261 gccacagtga tagagccctg cgatcgggcc gcgatagcgc gcgtagccgt gaacgggccg
     1321 cagcgaaaac agctgatcga gcgacatctg gccgtgcagg atgtcgcctc cgacgaggcc
     1381 gaactcgcgt tcgagatcca gcggcgacca tgcacgccag ccgagcaagg aacggcgaaa
     1441 gttcgggcag tacgactcca cgacgtcgaa gatcgcccgc accgcggcgt ccttcaaacg
     1501 ctcccagtcg gcgtcgggct tgaactgctg cgcaaacagg ctcgccacgt gcgcgccgcg
     1561 cggcgccagc gagtcgtcga gcacgctcgg gatcagcatt tcgacgatcg gcgtgcgtgc
     1621 gaaaccgtct acctgcgcgt cgcgccaagc acgatccatg caggcgagcg acggcgcgaa
     1681 cacgataccg ctcgcatgat gggcctgcgc ctgcgttccc ggcaggcacg cgaagtccgg
     1741 tagctccgac agcgcgacat tgatgcggaa cgaccctgac ccgcagcgat agcggtcgat
     1801 cgactgcgcg aagtcctccg gcagatcggc gcgcgcaacg aggcgctgat acaggagttt
     1861 cggattgacg tttgccgcca ccgcgcgcgc ccggatttcg cggcctccgc tcaacgcgac
     1921 gccgccggcg cggccgcgct ccaccagcac gcgttccacc ggcgcttcga cctcgatctg
     1981 caccccgcgc gcccgcgcct cgcccgccat caactccgtg atgcgcccca tgccgccgat
     2041 cgggtgaccc cattgcccgc gtcggccatg gatctcgccc agcgcgtagt gcagcagcag
     2101 atacgcggag cccggcgcgt atgggctcgc gtagttgccg accaccgcgt cccagccgag
     2161 cacgcccttc agcggctcgg actcgaacgt cgcgtcgagc cagtggccgg cgcttttcgt
     2221 caagaggtcg atcaggtcgc gctgactgac gcgcggcata cgacccaatt gcaccagcag
     2281 gcgcgctgcc gccagcgcat cgcgccaatc ccgcacgagc agcggccccc cgaggttcgg
     2341 cggcgcgcgc agcatccagc ccctcagcag cgacgccacg cgctcgcagc gggcgaaata
     2401 cgccggaagc gcggaggaat cgcgcaccga gaacttggcg acttctgccg cggcttccgt
     2461 tgcggagccg ccgaatttca ggaacgttcc ttccgcaagc ggcaggaaat ttccgaacgg
     2521 ccgctcgacg atgcgcagtc cacgctcgac gagcttcagg tcgcgaatga cccgcggatt
     2581 gagcaggctg accgtgtagc tggcgctcga attgcggaat ccggggacga actcctcggt
     2641 gaccgcggcg ccgccgacga cggatcggcg ctcgacgatg cggaccttca gcccggccgc
     2701 ggccaggtag caggcgcaga ccagaccgtt atggccggcg ccgatgacga gaacatcgca
     2761 gtccatcgcg cgccgacggc gcgctcaact gcctgcgtcc atctgcgact gcaggtagtt
     2821 ctgctgaccg acctggtcga tcagcgcaag ctgcgtctcg atgtagtcga tgtgctcttc
     2881 ggtgtcatcg aggatgtcga ccaggatctg gcgcgagacg aagtcgtggg ccgactcgca
     2941 cttggcgatc gctcccttca gcgtctgctg cgatccgcgc tcgagcgcga ggtcaccctt
     3001 caggacctcc ggcacgtcct gcccgatctg cagcttcgcg aggtcctgca ggttggggag
     3061 gccgccgagc agcagaatgc gctcgatgag ccgatcagcg tgcttcatct cgtcgatcga
     3121 ctcgtcgtac tccttcttgc ccatcttgcc atagccccac tggcgcagca tgcgagcgtg
     3181 caggaaatac tggttgaccg cgctgatctc gttcttcagt tgagcgttca gctgcgcaac
     3241 cacagccttg tccccttgca tcgcctgccc tcctgcgttg cgtgcgattc gtcgccgcgc
     3301 ctacgccgcc gaagcgccgc cgcgcgaaca ggtcgcgcag tcgccaccgc agcgacccgc
     3361 cgaaagaatg tactcctgcg cggcgtcgcg acagcatccg caaccagctc cgacgccgag
     3421 ttgcagcgag agttcgccca acgatcgcgc gcccgccgcc accgcagcct tcacctgctg
     3481 gtcgctgacc gcgttgcaga tgcagacgta catggtgtcc tctatcggta accgacgcgg
     3541 tcgaggagcc gctgcgcggc gatctggcct ttgccgatcg acgagatcgg aacgctctcg
     3601 tgcttgaacg taccgagcgc ggccagggcg ggattgatgt tctccgcacc cttgaccacc
     3661 ggccactcgt tgttgccctc ggcgaagtag gcctgcgcct ccttgctcga caggtattcg
     3721 aggaagcgga tcgcgttgtc acgatggggc gcatggcgcg ccacgccgcc gccggacacg
     3781 ttcacatgcg tgccgctcgt ggcctggttc ggccacacga agccgacctt ggcgaccacg
     3841 tccttgtctt tcgcgtcact cgagcgcacg agcctcaccc agtagtacgt attggtcagc
     3901 gccacgccgc attcaccgct cgcgaccgcg cggatctggt ccgtgtcgcc accacgtggc
     3961 ggccgcgcca tgttggcgac gacgcctttc gcccaggctt gggtcgctgc ttccccgctg
     4021 cgctcgacca tggcgccgat cagcgacagc atgtacgggt gcgagcccga gcgcgtgcag
     4081 acgagccctt tgagcttcgg gtcggcgagc ttctcgtacg tgtcgacgtc ggtcggctgc
     4141 agtcgcgact tgtcgtacac gatcacccgc gcgcgggtgg agaagccgaa ccagctgccg
     4201 tcgttcgagc gcagctcggc ggggatgcgc tcatccagta cctttgattt gatcggctgg
     4261 aacagcccat cgatctgcgc gcgccacagc cgcgctgcat cgaccagcaa gacgacgtcg
     4321 gccgggctgt ttgcgccttc gctcttcagc cgctgcaaca ggggctcgtc acccaactcg
     4381 atgcggttga tgccgatgcc tgtctgccgg gtgaagttct cgtacagggc ctcgtcggtc
     4441 tggtagtggc gcgccgagta aagattgagc actttgtcgc cgccggactg cgcgtggctc
     4501 gaccatggca gtgccgcccc gactgcaagc ccgagcgcgc agacagcgct cagcgctcct
     4561 tcgagccagc gttcggctcg cctgtcccct gcattcatcg ccgctccgat tcaggttgcc
     4621 ggcactgcgt gcaactctat cggaagccgt tctcaaatgc aaatcattct catttgattg
     4681 atgtcaacaa ggccgcagat tcaaggcctg ttgtccgcga cggagacggc atcgacgaac
     4741 gcttgcgtgc cggcgccgac cgatccgggc gtggcggaca cgtccggcga tccaccggcg
     4801 atgcgccgcg cgccgctgaa gcgcgcgcgc caataggcga actgcatcga ttcgatccgc
     4861 acccgcccgc cgcgtgccgg agcgtgcacg aaacgccctt cgccgatgta cacggcgaca
     4921 tgcgagaaag gccgacccag cgtgttgaag aacagcagat cacccggcgc aagcgactcc
     4981 tcgggcgcca ccgatcgccc gaccgtgccg attgcatccg agcgacgcgg caagtcgaac
     5041 ccgagcgcaa cccgcacgac gtgccgcacg agtccgctgc aatcgaagcc acgcgctgga
     5101 tcctcgcctc ccagccggta cggcacgccg aggaaactca atgcgcgcat cacgatctcg
     5161 gcgccggccg acggctcggc caccgcctcg tccgcgcgtg acggcaccgc aaccgcgagt
     5221 gccatggtgc agccgagcgc gatcaacccc gcgcgcaagc gcgcggggcg tgggccgaag
     5281 cgcagggatg agcgcaaatg cgtgaccatg atcagacgaa cggtgaattc atgtcgccaa
     5341 gatagcgaaa gacttcgcaa aatcccagcc tgcaatgcca cgaatcggcg gtggatcggc
     5401 cgtttcgggt aaattccgga gccctgtagc gtcgcggaag caccgcgcgc ctgcgagtga
     5461 ttgcgtgtcg gacgacgcca acttcaagaa cacagcgcct ctgccgcccc gggcgcggct
     5521 gctgatcgcg gacgattcgc gcatcgtgcg cgcgtcgatc acccagcata tccgcgaccg
     5581 tttcgatatc cgcgaggtca ccgacggcga ggccgcctgg caggcaatcc tgctcgacgc
     5641 atcgatccgc gtcctgatca gcgacctgac gatgccgaag atcgacggct tcgaactgtt
     5701 gtcgcgcatt cgcagctcga aggttcagcg catccgcgag ttgccggtcg tggtgatcac
     5761 cggcgccgac gaggaggcag agcgcgagcg cgccgccgcg ctcggtgcaa ccgacttcat
     5821 ccagaagggc gtgaacgcgg cggaactgat ctcgcgcctc gaggttctgg tgaagctttc
     5881 gaccacgcgc gagacgttgt ccgagacgca ggccacgctc gaatcggcgc gcacgatcga
     5941 cccggacacc gaacttcaca acctggtctt cttcgaccgc caggtcgaca agctgatctc
     6001 gtactcgcgc cgcaatcttt cggacgtcgc gctgatctgc atacgcgtgg aactctcgat
     6061 gccgcaatcc gactcatggg aaggcgagac ggagagccgc gtgaagctgg tcggtcgcac
     6121 gctcgcggca tcgattcgcc tggaggatct gggcacccgt tcggaccgct acgaattctg
     6181 cgtcgccaca cagaccaacg gtctgtcggg cgtgctgcga ttctcggcgc gcctgcgcaa
     6241 ggtgctggaa aacgtcgacg cggccggacc gggcgtcgag gtgtggacct gcatcggcgt
     6301 agcgacgctg tcggaggacc tgcgccgcgg cgcggaggaa ctgcgcgcga tcgcgcagcg
     6361 gcgcgcgcag caggcgcagg cgaaccgatc gcgtcggatc gtgctcggac cggccgaagg
     6421 cgcggcatcg ggctcggccg acgcgcggcc cgacgaagtg tcgatggacg tgagcctcgc
     6481 gctcgccgtg atccgcgccg gacgcgcgaa cgaggtcgtg cctcacctgc cgcggatcct
     6541 ggcgcagatc gagccgctgc tgaaactggt gcggcaacaa cgagaaatcc agaccaaccc
     6601 ttcggaggga gcacgcgatg accagctaca aggagttcca cgctaagtcg atctccgacc
     6661 ccgagggttt ctggggcgag caggcgcgcc tgatcgactg gcagacgccg ttttcgaaag
     6721 tcctcgacta ctcgaagccg cctttcgcga agtggttcgt cggcggccgg accaacctgt
     6781 gccacaacgc gatcgaccgc catctgcaag cgcgcggcga ccaggcggcg ctgatctacg
     6841 tctcgagcga gacgaatcag gacaagacat acaccttccg ccagctgcat cgcgaggtca
     6901 atcgcttcgc cgggatcctg aaaggcctcg gcgtcggcca cggcgaccgc gtgctgatct
     6961 acatgccgat ggtgcccgaa gcggtgttcg cgatgctcgc ctgcacgcgc atcggcgcga
     7021 tccattcggt ggtgttcggc ggcttcgctt cggtcagcct cgccacgcgc atcgacgacg
     7081 cccagccgaa ggtcatcgtc agcgccgatg cgggctcgcg cgccggcaag gtggtgccgt
     7141 acaagggcct gctcgacgag gcgatcacgc tcgcaaagtt tccgccgcgc aacgtggtga
     7201 tgatcaaccg cggactggcc gagatgagcc tcgtcgccgg ccgcgatctc gactacgcga
     7261 agctgcgcga aaaacacatg aacgacgagg tgccgtgcga atggctcgag tcgagcgaac
     7321 cctcgtacgt gctgtacacc tcgggaacga ccggcaagcc caagggcgtg cagcgcgaca
     7381 ccggcggcta tgcggtcgct ctgacttcat cgatgcagtg catcttctgc ggcaatgcgg
     7441 gcgagaccta tttctccacc agcgacatcg gctgggtggt cggacacagc tacatcgtgt
     7501 acggaccgct gctggcgggc atggccaccg tgctgtacga gggcacgccg atccgtcccg
     7561 acggcggcgt gtggtggagc atcgtcgaga agtacaaggt cacgtcgatg ttctcggcgc
     7621 cgaccgcgat ccgggtgctg aagaagcagg acccggcatt cctgcgcaag tacgacctgt
     7681 cgtcgctgcg cctcttgttc ctcgcgggcg agccactgga cgagccgacc gcgcgctgga
     7741 ttgccgacgg catcggcaag ccgatcatcg acaatttctg gcagaccgaa accgggtggc
     7801 cgatcctcgc gctgtgccgt ggcgtggagg cgctgccgcc gaagttcggc tcgccggggc
     7861 tgccggtcta cggatacgac gtgcggctgt acaacgagat gaccggtgcc gagatcggcg
     7921 acaaccaaaa gggcgtggtc ggcatcaatg gcccgctgcc gccaggatgc atgagcacgg
     7981 tgtggggcga tgaccagcgt ttcgtcgata cgtactggaa gaccattccg aaccggatgg
     8041 tctactcgac cttcgactgg ggcattcgcg gcgccgatgg ctacttcctg atcctcggcc
     8101 gcaccgacga cgtgatcaac gtcgccgggc accgtctggg cacacgcgag atcgaggagt
     8161 cgatcagttc gcaccccaac atcgccgagg ttgcggtggt cggcgtcgcc gatcagctga
     8221 aaggccaggt cgcgatggcg ttcgcggtgc tgaaggatca cggcaaggtc gccacgccgg
     8281 agcttcggcg cgcacacgag ggcgagatga tgaaggtcgt cgacgatcag ctcggcgcgg
     8341 ttgcgcgacc ggcgcgcgtt catttcgtct ccgtgctccc gaagacgcgc tccggcaagc
     8401 tcctgcgccg ctcgatccag gcgatctgcg aagggcgcga ccccggcgat ctcacgacga
     8461 tcgaggatcc gaccgcgctc gagcaggtgc gcacggcgtt gaagtcgggc tgatcgcgct
     8521 gcggtgatcg acttcacgtt ggagacgttc agtctcagct atttcctcgc gtcgttcctg
     8581 ctgccgccga tcctgctgct gatcaccgcg atcatcggcg cggcgcggtt gtggcggcag
     8641 ccgtattcct ggccggcgcg cgtgctgctc gcgctgtcgc tggccggaat gctcatattg
     8701 agcctgccgg tggtcgcatt ctcgctgttc aatccgctcg aggtccggcc gctcgattcc
     8761 gcacaggcca gggcggcgca ggcgatcgtg atcgtcgctg gcggtcgcgc gcggggcgcc
     8821 atcgaatggg gcggcgacac cgtcaacggc ttctcgctga cacggctgcg ctacggcgcg
     8881 cacctcgcgc gtaggaccgg tctgccggtg ctcgtgaccg gcggcggcgg gacggctgag
     8941 cgagcgcccg aggcggtgct gatgcagcgt gcgctgcagg gcgaattcgg catcgcgccg
     9001 cgctgggtcg aaagcgcaag ccgcacgacc gcagagaacg caaggttcag tgccgcaatg
     9061 ttgcgcacgg ccgggatcac gcgtgtgctg ctggtcaccc acagcttcca catgcggcgc
     9121 gcggcagcac agttcgagcg cgccggcctg caggtgattc ccgcgccgac ggggttccag
     9181 ggtttgcgcg atttcgaatg gctgcacctc gtcccctcgc cggacgccct gcgcctgtcg
     9241 cacgtcgcgc tgcgcgagtg gctggcgctg gcgcgcgatc ggctaatctc gcacttcggg
     9301 tcctgaagga gcatgcgatg cagagccgga agtatctgac gctggatgac gtgcagagga
     9361 tcggcgccgc cgcgaccgcg gaggcgcaga agaacaaatg ggcggtcacg atcgcgatcg
     9421 tcgacgacgg cggccatctg ctgtggctgc agcgcctcga cggcgccgcc accgcatccg
     9481 tgtacatcgg gcaggagaag gcgcgctcgg ccgccctcgg ccggcgcgag accaaacgct
     9541 atgaagacat gatcaaccag ggacgcaccg cgttcctgtc ggcgccgctg gcgggcatgc
     9601 tcgaaggcgg cgtgccgatc gtcgccgaag gccacgtgat cggcgccgtc ggcgtatccg
     9661 gcgtgcagtc gagcgaagac gtccagatag caaaggcagg tatcgcggcg ctcggcgtct
     9721 gattcattcg atctgccccg ctcgccgccc tcgctcgacg tcaatcggcg ccagcagcgc
     9781 gagcccgact acgaaataca ggcccgtcac caggatcgcc agccggtgat cgttgtcggt
     9841 gatccaggtg accagaccgt aggtcatcgg gccaaccaca cccgagagcc actgcgccgc
     9901 attccaaagc gcatagaact ccgccagccg tgccgtcggc gcgaacacgc ccaccagcgc
     9961 gcggccggcc gactggctcg atcccatgca cagcccggcg atgttggcag cgatccagaa
    10021 gccggtcgtg ccggtgctca gcgccgcgat cagcaccatc gcgatccagc cggcaatcgt
    10081 gatcgccagt gtggcgcgat gtcccagcgc atcctgcacg tagccgaaca ggaacgcgcc
    10141 gattgccgcc gtgatgttga ccagcaggat cagcagcatc gtctgcgcca tcgtgaagtg
    10201 catcgcctgc tgtgcgtaga tggcggccag cgcgatcacg acggcgatgc caccctgata
    10261 gaagaatccg cacaccagca gccagccgaa gtcgcgatgc gcgccgatgt tcttcagcgt
    10321 atgccacatg cgctgcgtcg attcggcgag gaagtttccg accgaaacgc cggcacgcgg
    10381 cacggcgcgc tcgcgcacga gagcaaacat cggtgcggcc gccaacgcga acaccgccgc
    10441 ggtgatcagc atcgtcaccg gcacgaattg cgtggcggtc tggccgcgcg cctgcgccca
    10501 ttggacgtag cccagaccca gcccgagcgc cagcagcccg ccaaggtagc cgaagctcca
    10561 gccccacccc gagaccttgc cgagtgcctc gggcctcgcc agttccggca ggaacgcgct
    10621 gttcagcgtc acgccggccg agaaacaaaa gttcgaaacg accagcgcgg ccgccgccag
    10681 tgccagatcg ccaggaccca cgaacgcgag cgcaaccgtg ccgacgatgc agcccacggt
    10741 gaccacgagc agcatcagct tcttcttcgc atgcaggtcc gcgtaggcgc cgagcggcgg
    10801 catcagcacc atgcccacca cgtaggaggc cgacaacgcc agcgtccacg ccagcgtggc
    10861 ccagtcggca ccgcgcgcga ccacgctgac gaaatacgcg ttgaacaccg ccgtcagcac
    10921 gacggtcgta tagccggagt tcgcgaagtc gtagcacgac cacgcgaaga cttcgcgccg
    10981 ggtgacgccg gggttcagcg cttgcggatc gaagagcggc atgggtcgcg catgctaccg
    11041 ccgcacctat actcacgcgc gcaatgaaga ccatcgaccg catcgaccgg cagatcctgg
    11101 aggtcttgca gcaggatggc cggctgacga accaggcgct gtccgaacgc gtggctctgt
    11161 cgccgcgcgc gtgcctggaa cgagtgcgcc gcctcgagaa ggcgggctac atctccggct
    11221 acatggcgct ggtcgagccg cgcaagctcg gcggcctgct gacgatcgtg gtcgaagtct
    11281 cgctgcgcga ccaatcggct gccacgcacg cgcgtttcga ggcccggatg cgagccgcgg
    11341 acgaggtgat cgagtgcttc ctcgtttcgg gagcatgcga ctacgtgctg cggctcgcgt
    11401 gccgcagcct cgatcactac cgcgatctca ccaacgcgtg gaccgacgac ccgacgctcg
    11461 gcgtggagcg catcgcctcc aagccggagc tgcagaccgt caagccgttt cgcggatatc
    11521 cgattccctg acggccgctg cgtcgcccgc agtcgaaact gccatcagca ggtttgcccg
    11581 cgccgcggcc tcgaacgcct tgctcgcgag cggcgtgcgg tagatcgacg cgcgcgcgag
    11641 caaccgctcg aagaacggtc gccgcagccg gcagaaatag cggtacgccg cctcgtcgtc
    11701 ctcgagccgg atcagcgcgc gctgctccgc cagcaccgcg cgcgaatagc gctcgaagtg
    11761 cagcggtggc gccgccagcc gcagcagatc gaggtcgagc accagttccg cctcgagcga
    11821 acgcgttgcg tgctcggtcg tgtcgagcac gatctgcgcc gcgcgctcga tgcagtcgag
    11881 cgcgagcccg tgcgcaaacg cgcgcagcag ttgtgccgac atggcctcgt tggtcccgcg
    11941 ggccgcgccc ggcacgtaga ccgcgtcgtg gaacagcagc gccagcgatt gtgccggcgt
    12001 cagcgtcacg ccgagaccga gcgccgcgtc gagcatctcg ccgatgtgct cgcgatcgtg
    12061 atagtggcga tgcggctcgt cgtagaagac cagcacctgg tcgatacgct gcgcgtgaaa
    12121 gcccgcgcgg cgcaggctgg ccagcgcggg atgggtaccg gtgggtgtca tgaaataggt
    12181 cgtcatgccg tcatcgtagg cagccgcgtc gtgccggcca acgaaaatcg gcgtgaatat
    12241 tcgaccgcct cggcacgccc gtgcgatcgg gcgatgcgct gcagattcgg cggtctcgtg
    12301 tcgcgcgcgc ggccgaagcg gaatccgcag cgcatcactt tcgccgctgt cgaagcgcat
    12361 cttccctaga cttttcgtga tgcggtacga agaccgcgca agaggcaaat gatggatatc
    12421 cgacgcaccc tggctaccga ggcaatcgcg cgcgaagccg cagtcgccgc gcccaattac
    12481 cacccgcttc cggtcgtact gcagagcggc cgcggcgaat ggctgacgga tgtcgacggc
    12541 aagaggtacc tcgacatgat gagcgcgtat tccgcggtca gccatggcca cgcgcatccg
    12601 cgcctcgtcc gcgtcctgat cgaacaggcg cagcgcgtcg cagtcacgtc acgcgcgtat
    12661 cacaccgaca cgctcgcgcc cttcctcgag aagctcaccc aactgacggg cctggaccgt
    12721 gcgttgccgg ccaacggcgg cgccgagtcg gtcgaaaccg cgatcaaggc ggcccggcgc
    12781 tggggctatc gcgcgcgagg aatcgctagc gatcgggcgg agatcgtcgt ggcccgcggc
    12841 aacttccatg gccgctcgac gacgatcgtg agcttctcga cggaggcggg ctatcgcgac
    12901 gggttcggcc cgttcacgcc gggttttcgc catttcgatt tcggcgatgc cgagtcgctc
    12961 ggtgccgcga tcaacgagag aacgtgcgcg gtgctgatcg agccgatcca gggcgaggcc
    13021 ggcatcgtgg tgccaccgga cggattcctg cgcgcggcac gacgactgtg cgacgagcgc
    13081 aaggtcctgc tgatcctcga cgaaatcc
//
LOCUS       DBNHBY010000210         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_823463, whole genome shotgun sequence.
ACCESSION   DBNHBY010000210 DBNHBY010000000
VERSION     DBNHBY010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_823463"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC16HB_05135"
     CDS             complement(<1..437)
                     /locus_tag="AC16HB_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5606209.1"
                     /translation="MNRRVAVVGAGWSGLACALTLAERGARVTLIDAAPQAGGRARRV
                     EVALGDATYALDNGQHLLVGAYRDTLRLIARVGLDTGQVLLRQPFALRYPDGFLLQVG
                     RWPAPWHLAAALVNARGFTGADRVSIARDVHAWRRSGWQAARDA"
     gene            complement(434..1273)
                     /gene="hpnD"
                     /locus_tag="AC16HB_05140"
     CDS             complement(434..1273)
                     /gene="hpnD"
                     /locus_tag="AC16HB_05140"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR5606210.1"
                     /translation="MTPDEYCQKQAAQSGSSFYYSFLFLPPERRRAITALYAFCREID
                     DAVDEASDSSVAGAKIAWWQSEIERLYAGEPQHPVTRALAPHVTAFDITRERLLGVLE
                     GVRMDLTQTRYLDFTALARYCHLVAGIVGELSAGIFGMTDPRTFEYAHTLGLALQLIN
                     IVRDVGEDARRGRIYLPLDELQRFGVKAADLLNARYVEGYVPLMKFQAQRARETYVAA
                     RRLLPEADRRAQRPGLIMASIYVALLDELERDGFPVLHQRVSLTPLRKLWIAWRTWAF
                     TGR"
     gene            complement(1270..>1927)
                     /gene="hpnC"
                     /locus_tag="AC16HB_05145"
     CDS             complement(1270..>1927)
                     /gene="hpnC"
                     /locus_tag="AC16HB_05145"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIR5606211.1"
                     /translation="AQLAALERQLDAIAAGRAHDWPDLAAAIRAHGLPLEPLRHLLSA
                     FAQDVRVHRYADYDALLDYCRRSANPIGRLLLALYRCADATSCAQSDAICSALQLTNF
                     WQDVAIDFERGRVYIPQSELRRFGLSTDDLVARRVDARWTALMRSLTAHARAMLSSGA
                     PLARSLGGRIGLELRLIVEGGLRILERIEAVDGDVFSRRPTLRAADWLLMGARCAWRR
                     "
BASE COUNT          314 a          677 c          678 g          258 t
ORIGIN      
        1 gcatcacgcg cggcttgcca gccgctgcgg cgccaggcgt gcacatcgcg cgcgatcgaa
       61 acccggtcag cgccggtgaa gccgcgcgcg ttcacaagcg ccgccgccag atgccagggc
      121 gcgggccaac ggcccacctg gagcaggaag ccgtcgggat agcgcaaggc gaacggctgg
      181 cgcagcagaa cctgcccggt gtcgagcccg acgcgcgcga tcagccgcag cgtgtcgcgg
      241 tacgcgccga ccagcagatg ctggccgttg tcgagcgcgt acgtcgcatc gccgagcgca
      301 acctcgacgc ggcgcgcgcg gccgccggcc tgcggcgcgg catcgatcaa cgtgacacgc
      361 gcgccgcgct cggcgagcgt cagcgcgcac gcgagtccgg accagcccgc gccgaccacc
      421 gcgacgcgcc ggttcatcga cccgtgaagg cccacgtgcg ccacgcgatc cacagcttgc
      481 gcagcggggt cagcgacacg cgctggtgca gcaccggaaa gccgtcgcgc tccagttcgt
      541 cgaggagcgc gacgtagatc gaggccatga tcagtcccgg ccgctgggcg cggcggtccg
      601 cttccggcaa cagccgccga gccgccacgt acgtctcgcg cgcgcgctgc gcctggaact
      661 tcatcagcgg cacataacct tcgacatagc gcgcattcag caggtcggcc gccttcacgc
      721 cgaagcgctg caactcgtcg aggggcagat agatacggcc gcggcgcgcg tcctcgccga
      781 cgtcgcgcac gatgttgatc agctgcagcg ccaacccgag cgtgtgcgcg tactcgaacg
      841 tgcgcggatc ggtcatgccg aagatgccgg ccgacagctc gccgacgatg cccgcgacca
      901 gatggcagta gcgcgccagc gccgtgaagt cgaggtagcg cgtctgtgtc aggtccatcc
      961 gcacgccctc gagcacgccc aggagtcgct cgcgcgtgat atcgaacgcc gtgacatgcg
     1021 gtgccagcgc gcgtgtgacc gggtgctgcg gctccccggc gtaaaggcgc tcgatctcgc
     1081 tctgccacca cgcgatcttt gcgccggcga cagaagagtc cgaagcctcg tcgaccgcat
     1141 cgtcgatctc gcggcagaac gcgtacagcg cggtgatcgc gcggcgccgc tcgggcggca
     1201 ggaacagaaa gctgtagtag aagctggagc cgctctgcgc tgcctgcttc tgacagtatt
     1261 cgtcgggtgt catcgtcgcc atgcgcagcg agcgcccatc agcagccagt ccgccgcgcg
     1321 cagcgtgggg cgccggctga acacgtcgcc gtcgacggct tcgatccgtt ccaggatgcg
     1381 caggccgcct tccacgatca gccgcagttc gagtccgatg cggccgccga gggagcgcgc
     1441 caatggggcg ccggacgata gcatcgcgcg cgcgtgcgca gtcagcgagc gcatcagcgc
     1501 ggtccagcgt gcatcgacgc ggcgcgccac gaggtcgtcg gtcgacaggc cgaaacgccg
     1561 cagttccgac tgcgggatgt agacgcgccc tctttcgaag tcgatcgcga cgtcctgcca
     1621 gaagttggtg agctgcagcg cgctgcagat cgcatcggat tgcgcgcagg aggtagcgtc
     1681 ggcgcatcgg tacagcgcca gcagcaggcg cccgatcggg ttggcggaac ggcggcagta
     1741 gtcgagcagc gcgtcgtagt ccgcgtatcg gtgcaccctg acgtcctgcg cgaaagccga
     1801 cagcaggtgg cgcaagggct ccagcggcag gccgtgcgcg cggatcgcgg ccgcaaggtc
     1861 cggccagtcg tgcgctcgtc cggcagcgat cgcgtcgagc tgcctctcga gcgcggccag
     1921 ttgcgcg
//
LOCUS       DBNHBY010000211        12071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1365233, whole genome shotgun sequence.
ACCESSION   DBNHBY010000211 DBNHBY010000000
VERSION     DBNHBY010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1365233"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="AC16HB_05150"
     CDS             <1..1432
                     /locus_tag="AC16HB_05150"
                     /inference="COORDINATES: protein motif:HMM:NF051647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR5606212.1"
                     /translation="SRCARAAQQRARTTSGHLRLAIDRVFTLAGIGTIVTGTAHAGCV
                     RVGDEVRLQPSGRMARVRSIHAQDRSADAGCAGQRCALNLAGIAKDDAARGEWVQAEA
                     LANTTARFDAALRLSPRAARALPAWSSVHLHHGTRDVLARVAVLDAERIEPGAEQLVS
                     ITLEAPLAACKGDRFVLRDASARRTLGGGIVLDIAPPARGKRRPERLALLHALHDLPA
                     RDALAAWLAAEPVPLARLVGGWNLLDADVDELLHAVDARVAAGTAFSPARWDALRSTV
                     LAAVAANHQREPEMPGLEQNRLRRMVAPAIAADAFAELIDELLGGGKLVRRGAFVALP
                     THRAELGKEERIQWERIQPLLMNERFNPPRVRDIARATGCAETELRSLLRRVARVGAV
                     ALVAHDHYFLTDALREMADIAGELMASRGVARAAEFRDRIGTGRKVAIQILEFFDRVG
                     YTRRVRDDHVPRRDNPWRAEQEGRVP"
     gene            1418..1513
                     /locus_tag="AC16HB_05155"
     tRNA            1418..1513
                     /locus_tag="AC16HB_05155"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Sec,seq:tca)
     gene            complement(1608..1967)
                     /locus_tag="AC16HB_05160"
     CDS             complement(1608..1967)
                     /locus_tag="AC16HB_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606213.1"
                     /translation="MQCVQPAEAELQCLGVLETCHQAEVLAQMAQEARVIHLAFEDGM
                     NSGLALADELERALGQALDAEEPDESSAAHGELMRVLDRLRGFGLQVSASVSELSIDG
                     VLGAVRMPVLTAKLLAV"
     gene            complement(2122..2322)
                     /locus_tag="AC16HB_05165"
     CDS             complement(2122..2322)
                     /locus_tag="AC16HB_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5606214.1"
                     /translation="MNRLLIVLGLALLVIGLAWPWLGRIPWGRLPGDIFVQRENFSFY
                     LPLATGLIVSIVLSVVIWLLRK"
     gene            complement(2341..5958)
                     /locus_tag="AC16HB_05170"
     CDS             complement(2341..5958)
                     /locus_tag="AC16HB_05170"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006228000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5606215.1"
                     /translation="MNAPLPEAVRRALAEVTLDDKWTLERGRAYMSGTQALVRLLMLQ
                     RQRDLAAGLNTAGFLTGYRGSPLGGVDQTAWRAKKHLETHHIKFQPGVNEDLAATSVW
                     GTQQVNLFGGARYDGVFGMWYGKGPGVDRCSDVFKHANAAGTSPHGGVVALAGDDHGA
                     KSSTLPHQSDHIFKACMIPVLFPATVQEYLDLGLHAYAMSRYAGVWVAMKCVTEVVEV
                     SASVTVEPERVTIAAPTDFAMPTGGLAIRWPDTPLAMEARLLDYKLYAALAYCRANRL
                     NYTAIDSPHARFGIVASGKAYLDTRQALLDLGLDEATCRDIGIRLFKCGMVWPLEAQT
                     LGTFAEGLEEILVVEEKRQMIEYQVKEELFNWIGKGKRIPRVIGKFDEHDGGEWSVPR
                     GDWILPAHYEFSPVIIAKAIATRIGKLELPRDVRARMQARLAIIEAKERALARPRVVT
                     ERTPYFCSGCPHNTSTRVPEGSRAAAGIGCHYMALWMNRNTATFTQMGGEGTPWIGQA
                     PFTSEKHIFANLGDGTYFHSGSLAVRAAVAARVPITYKILFNDAVAMTGGQPTDGPLT
                     VPQITQQMYAEGVRRIVIVTDEPGKYDATRELDHSALNARGAGEDAWRLAPGVTVHHR
                     DDLDRVQRELREYPGVSVLVYDQTCASEKRRRRKRNEFPDPPRRVVINELVCEGCGDC
                     SVQSNCLSVEPLETEFGRKRTINQSSCSKDFSCLKGFCPSFVTIEGGTPRKGKSASGA
                     SLPALPAPHVPELPQGRNYGVVITGVGGTGVVTIGQLMGMAAHLEGKGLSVLDMAGLA
                     QKGGAVFSHVQIAPRPEELFATRIAMGEADLLLGCDLIVSTSNEALAKIHAGRTKAVV
                     NSSESPTADFVRNPDWQFAGGTMAQQLREAVGNDAHSAFVDANALTTALMGDAIYTNP
                     FVLGYAWQKGWVPLQHETLLRAIELNGVAIEANQRAFDWGRAAAHDLDAVKKAAFPAQ
                     VIELKRPASTLAEVVARRVEFLTGYQNAAYAKRYADLVERVRKVESDRLHGATKLAEA
                     VARGYFKLLAYKDEYEVARLHADPAFRARIAAQFEGDFTLNFYLAPPLLAKPDPATGQ
                     VRKMRFGSWMMTAFAVLARLKFLRGTAFDVFGRTEERRTERALIREYEQLVDELLSRL
                     AGDNHEFAVQLASVPDEIRGYGQVKAANLETARAKWAGLLAKFRGQSSAQVIQMPVKA
                     A"
     gene            complement(6136..6549)
                     /locus_tag="AC16HB_05175"
     CDS             complement(6136..6549)
                     /locus_tag="AC16HB_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR5606216.1"
                     /translation="MNATPSAVAPEPGLVRVAHIVYALHTLSLVIGAFGAATIVGAFV
                     FGWPSIIAVIINYVKRGEARGTWLESHFRWQIRTFWFALLWAVVVAVLSVLLAVVLIG
                     IALWVVGLFALGIWAIYRIARGWLALKDGRALPPG"
     gene            complement(6629..7615)
                     /locus_tag="AC16HB_05180"
     CDS             complement(6629..7615)
                     /locus_tag="AC16HB_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5606217.1"
                     /translation="MPPAAVHPRISLDQWRALAAVVEAGGYAQASARLHKTQSSVSYL
                     IQKIERLLDVKLFTVEGRKAHLTAHGEVLYRRARALLDEAEAIERSARTLAAGWEPEL
                     RIAADIVFPTWLLLRAFARFAEERPETRIQLYETVLGGTDEALVERRVHLGINTSVPP
                     GLVGDTLMQMRFIAAAHPDHPLHRLGRPLTYRDLRTHRHLIVRDSGLTMTREGGGWRG
                     AEQRWTFSNKATSIFAAGMGLGFAWFPEETIRGELERGALKLLPLREGAERYVTLYLI
                     VADGDAAGPGTRRMAQIVREEVDATCAARGAALSRPPAEVPARKLRQRRRSR"
     gene            7718..8341
                     /locus_tag="AC16HB_05185"
     CDS             7718..8341
                     /locus_tag="AC16HB_05185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase (NADP+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HIR5606218.1"
                     /translation="MKTLLQINTSLFGAGGNSTQLADNFVAAWKALNPGGRVIVRDLA
                     RDPIPHLAAEHVQAFGMPAAQRTAVQATIVARSNALIEELKSAHVVVLGLPMYNFGVP
                     SVLKSYFDQIARAGVTFRYTANGPEGLIGDRPTYVFAARGGMHRDTPRDLQTGFVTTF
                     LNFIGIRSIEFVYAEGLGYGDEAKTKSLSAARRQIAEIVGAVELAAA"
     gene            complement(8342..9502)
                     /locus_tag="AC16HB_05190"
     CDS             complement(8342..9502)
                     /locus_tag="AC16HB_05190"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5606219.1"
                     /translation="MLRPSLDALPASRIREVANTAIGRADLVKFWFGEGDLPTPEFIR
                     NAAKASIDAGETFYNHNLGIIELREAVAGYVDRLHAPREPIGVDRIAITSAGVNALSL
                     VSQALIEPGDRVAIVTPVWPNVTAQPQIVGGRVARVPLTCANGTWTLDLQRLIDAAPA
                     GTRMVVVNSPGNPTGWVMPQEQWNALLAHCRRHGIWLVADDAYERIIFDGSAHAPGVL
                     AQADPDERFISANTFSKSWTMTGWRLGWLVAPRSFISNFAKMVEFNTSCAPTFVQRAG
                     IVAIRDGDELIAQTVARLKGARDLLVGRLRALKGVEAASPPGSMYLFFRIPGRSGDSL
                     AFCKSLVTTVGLGLAPGIAFGPEGEGYVRWCFAATPGLIEQGVERRVRYLQV"
     gene            9621..10313
                     /locus_tag="AC16HB_05195"
     CDS             9621..10313
                     /locus_tag="AC16HB_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606220.1"
                     /translation="MAGRFKPVAFEPLGGRRSRIRVPRWLVLLLSGIALGVGGVVVVQ
                     ERYLPPRLTADESSQLRAAFEQADTERQRLAADLAETSKRLDKALADGKGLSDQLAAS
                     RATTDRLRDDLATVVAALPADPRGGTVEVRAGRFALQRGALAYDVVLTRERAAGKPMK
                     GVLQLTVAGESGRGGESTVALSPINVLIGSHEVVRGSAPLPEGFRPRQTTVQVLDRVA
                     GNSLGMRVMLVK"
     gene            complement(10306..11985)
                     /locus_tag="AC16HB_05200"
     CDS             complement(10306..11985)
                     /locus_tag="AC16HB_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606221.1"
                     /translation="MPSPPLAALERCRLQFGPAAADAKLALLRRLDRIRLGSARAVKR
                     LHEALCFIRAYPDDAAVLAQVQAMLAGFAGRADLRAVRAALADTGIAGTPIHYRFFAG
                     QAQWLAAKWPAWLRLDRSDTAADANIARALPPLLSSAESTALMELKLAGYAALDRLRG
                     DETDAAFLLRRIAAMPGNTFTREAFADAIDASFVLAPGPDTPSRTRAELRGAPVVFRR
                     APPPRVRPDLRAELLRAPRSVRRLSPRAGREVIDLARGAMVTRARSLEAFSFADARDA
                     WLVDDGDGLAFAFIGVLPERRQALASCYGSLTLRNGVPIGYMQSDHVGAGAALSFNTF
                     ESFRGGEAAFVFARWLAALRHAFGATSFSIEPYQLGKDNDEALESGAWWFYAKLGFAP
                     RAAATRALTRVERDRLRRDPRHRTRRATLLRLAARHLFFDLDSAHPRPLLSLGALGLL
                     AGVALSRRAGSDRERGVAQASEALMRQCGLASLRALTPDQREAWRRMAPILALLEVDA
                     WREAERLALADVIRAKGGRSERDFIARCLAHPRLEAALLRWARAQNGTAPT"
BASE COUNT         1868 a         4187 c         4171 g         1845 t
ORIGIN      
        1 atcccggtgc gcgcgcgccg cgcagcagcg ggcgcgcacc acgtccggtc acctgcggct
       61 cgcgatcgat cgcgtcttca cgctcgccgg catcggcacc atcgtcacgg gcaccgcaca
      121 cgcgggctgc gtgcgcgtcg gcgacgaggt gcggctacag ccgagtggcc gcatggcgcg
      181 ggtgcgctcg atccacgccc aggaccgcag cgccgacgct ggctgcgccg gccagcgctg
      241 cgcgctgaac ctcgccggca tcgccaagga cgacgccgcg cgcggcgagt gggtgcaggc
      301 agaggcactc gccaacacga ctgcgcgctt cgacgcggcg ctgcggctgt cgccgcgcgc
      361 ggcccgcgcg ctgcctgcgt ggagcagcgt gcacctgcat cacggcacgc gcgacgtgct
      421 ggcgcgggtt gcggtgctcg atgccgagcg catcgagccc ggcgctgagc agctcgtgtc
      481 gatcacgctg gaggcgccgc tcgccgcgtg caagggcgat cgcttcgtgc tgcgcgacgc
      541 gagcgcgcgc cgcacgctcg gcggcgggat cgtgctcgac atcgctccgc cggcgcgcgg
      601 aaagcgccgg ccggaacggc tcgcgctgct gcatgcgctg cacgatctgc ctgcgcgcga
      661 cgcgctcgcc gcgtggctgg ccgccgagcc ggtgccgctg gcgcggctgg tcggcggctg
      721 gaatctgctg gacgccgacg tcgatgaact gctgcacgcg gtcgatgcgc gcgtggcggc
      781 cggcaccgcg ttttctcccg cacgctggga tgctttgcgc agcacggtgc tcgccgcggt
      841 cgcggcgaac catcagcgcg agcccgagat gccggggctg gagcagaacc gtctgcgccg
      901 catggttgcg cctgcgatcg cggcggacgc gttcgcggaa ctgatcgacg aactgttggg
      961 cggcggcaag ctggtccgcc gcggcgcatt cgtcgcccta cccacgcacc gggccgaact
     1021 gggcaaggag gaacggattc agtgggagcg catccagcca cttctgatga acgagcgctt
     1081 caatccgccg cgcgtgcgcg acatcgcgcg cgcgacgggg tgcgcggaga ccgagctgcg
     1141 cagcctgctg cggcgcgtgg cgcgggtcgg cgcggtcgcg ctggtcgcgc acgaccacta
     1201 ctttctgacc gacgcgctgc gtgagatggc cgacatcgcg ggcgaactga tggcgagccg
     1261 cggcgtggcg cgcgcggccg agttccgcga ccgcatcggc actgggcgca aggtggcgat
     1321 ccagatcctc gaattcttcg accgcgtggg ctacacccgc cgcgtgcgcg acgatcacgt
     1381 gccgcgccgg gataatccgt ggcgagcaga gcaggaagga agagtcccgt gacccggtgg
     1441 tccggccaga cttcaaatct ggtaggggtt gccagcaatc cctggtgggt tcgactccca
     1501 tgctcttccg ccaaggcgca tcgatacggc cgacgtctcg tgcgcggcgc cgagccgacc
     1561 gcaatcgaca gcgcggccgg gcccagtgag cgaacgcggg ccttcttcta gacggccaag
     1621 agcttcgcgg tcagcaccgg catccggacc gcgccgagca cgccgtcgat cgacagctcg
     1681 gacaccgacg ccgacacctg caagccgaaa ccgcgcaacc gatcgagtac gcgcatcagt
     1741 tcgccgtgcg cggcgctcga ttcgtcgggc tcctcggcgt cgagcgcctg gccgagtgcg
     1801 cgctcgagct catcggccag cgccagaccg gagttcatcc catcctcgaa cgcgaggtgg
     1861 atcacgcgtg cttcctgcgc catttgcgcc agcacttcgg cctgatggca cgtttcgagc
     1921 acgcccaagc attgcagttc ggcttcagca ggttgaacgc attgcatggc ggtctccagc
     1981 ggggcaggtt cgactccgag cgaaccgtag tggacggcgc cgcggtcggc cacgattttt
     2041 cgagcaaagt ttgaccggtc gggaaaaagc ttgatcaagc gcaaatgggg acgcgcaccc
     2101 cgccaatgcg gggtgcgggg gtcacttgcg gagcagccag atcacgaccg acagcacgat
     2161 cgagacaatc agcccggtcg ccagcgggag gtagaagctg aagttttccc gctgtacgaa
     2221 gatgtcgccg ggcaggcgtc cccacggaat cctgccgagc cagggccacg ccaggccgat
     2281 caccagcagc gcgaggccaa ggacgatcag cagccggttc atgcgccgcc ctcgtggcca
     2341 tcaagccgcc ttgactggca tctggatcac ctgcgcagaa ctttgccccc ggaatttcgc
     2401 gagcagtccg gcccatttcg cgcgtgccgt ttcgaggttc gcagccttca cttgaccgta
     2461 gccgcggatc tcatccggta cgctggccaa ctgaaccgcg aattcatggt tgtcgccggc
     2521 cagccggctc agcaactcgt cgaccaactg ctcgtattcg cggatcagtg cgcgctcggt
     2581 gcggcgctct tcggtacggc caaagacgtc gaacgcggtg ccgcgcagga acttcagcct
     2641 cgcaagtacg gcgaaggcag tcatcatcca cgagccgaag cgcatcttgc ggacctgccc
     2701 ggttgccggg tcgggctttg ccagcagcgg cggggcgaga tagaagttca gcgtgaaatc
     2761 gccctcgaac tgcgcggcga tcctggcgcg gaacgccggg tccgcgtgca gccgcgcgac
     2821 ctcgtactcg tccttgtacg cgagcagctt gaagtagccg cgggcgaccg cttcggcgag
     2881 tttcgtcgcg ccgtggagcc ggtcggattc gaccttgcgc acgcgctcga caagatccgc
     2941 gtagcgcttc gcgtacgcgg cgttctgata gcccgtcagg aattcgaccc gccgcgcgac
     3001 gacctccgcc agcgtcgaag ccggccgctt cagctcgatc acctgcgcgg ggaatgcggc
     3061 tttcttcacc gcgtccaggt cgtgtgcagc cgcgcggccc cagtcgaacg cgcgctggtt
     3121 ggcttcgatc gccacgccgt tcagctcgat cgcacgcagc agcgtttcat gttgcagcgg
     3181 cacccagccc ttctgccacg cgtagccgag gacgaacggg ttggtgtaga tcgcgtcacc
     3241 catcagcgcg gtcgtgagcg cgttggcgtc gacgaatgcg ctgtgggcgt cattgcccac
     3301 cgcctcgcgc aattgctgcg ccatcgtgcc gcccgcgaac tgccagtcgg gattgcggac
     3361 gaagtccgcg gtcggtgact cggaggaatt caccaccgcc ttcgtgcgtc cagcgtggat
     3421 ctttgccaac gcctcgttgc tcgtcgacac gatcaggtcg cagccgagca gcaggtctgc
     3481 ctcgcccatc gcaatgcgcg tggcgaagag ctcctccggc cgcggcgcga tctgcacgtg
     3541 cgagaacacc gcgccgccct tctgcgccag ccccgccatg tcgagcaccg aaaggccctt
     3601 gccttcgagg tgcgcggcca tccccatcaa ttggccgatc gtgaccacac ccgtgccgcc
     3661 cacgccggtg atcacgacgc catagttgcg cccctgcggc agctccggca catgcggcgc
     3721 cggcagcgcc ggcaacgatg ctcccgacgc ggacttgccc ttgcgcgggg tgccgccttc
     3781 gatcgtgacg aagctcgggc agaagccctt caggcatgag aaatccttgc tgcaactcga
     3841 ctggttgatc gtgcgcttgc ggccgaactc ggtttcgaga ggctcgaccg acaggcagtt
     3901 cgactgcacg ctgcagtcac cgcagccttc gcacaccagc tcgttgatca ccacgcggcg
     3961 cggcggatcg gggaactcgt tgcgcttgcg ccgccgccgc ttctcggagg cgcaggtctg
     4021 gtcgtacacc agcaccgaca cgccgggata ctcgcgcagc tcgcgctgca cgcggtccag
     4081 atcgtcgcgg tggtgcaccg tcacgccggg cgcgagccgc cacgcgtcct cgcccgcgcc
     4141 gcgcgcgttc agcgcgctgt gatcgagttc gcgcgtcgcg tcgtacttgc ccggctcgtc
     4201 ggtgacgatc acgatccggc gcacgccctc cgcgtacatc tgctgcgtga tctgcggcac
     4261 tgtcagcggc ccgtcggtcg gctgcccgcc cgtcatcgcg accgcgtcgt tgaacaggat
     4321 cttgtaagtg atcggcaccc tcgccgcgac cgcagcgcgt accgcgagcg aaccggagtg
     4381 gaaatacgtg ccgtcgccga ggttggcgaa gatgtgcttc tcgctcgtga acggcgcttg
     4441 cccgatccac ggcgtgcctt cgccgcccat ctgcgtgaag gttgccgtat tgcggttcat
     4501 ccacagcgcc atgtagtggc agccgatgcc ggccgcggcg cgcgaacctt ccggcacgcg
     4561 ggtcgacgtg ttgtgcgggc agcccgagca gaagtacggc gtgcgctccg tgacgacgcg
     4621 tggcctggca agcgcgcgtt ccttggcttc gatgatcgcc agccgtgcct gcatgcgcgc
     4681 gcgcacgtcg cgcggcaatt ccagcttgcc gatgcgcgtg gcgatcgcct tggcgatgat
     4741 caccggcgag aactcgtagt gcgcgggcag gatccagtcg cctcgcggca ccgaccactc
     4801 gccgccgtcg tgctcgtcga acttgccaat tacgcgcggg atgcgcttgc ccttgccgat
     4861 ccagttgaag agctcttcct ttacctggta ctcgatcatc tggcgctttt cctcgacgac
     4921 gaggatctcc tccagcccct cggcgaacgt gccgagcgtc tgcgcctcca gcggccacac
     4981 catgccgcac ttgaagagcc gaatgccgat gtcgcggcag gtcgcctcgt ctagtcccag
     5041 gtcgagcagt gcctgccgcg tgtccaggta cgccttgccg ctggcgacga tgccgaagcg
     5101 cgcatgcggt gaatcgatcg cggtgtagtt cagtctgttc gcgcggcagt acgcgagcgc
     5161 cgcgtacagc ttgtagtcga gcagccgcgc ttccatcgcc agcggcgtgt ccggccagcg
     5221 aatcgcgagc ccccccgtcg gcatcgcgaa gtcggtcgga gccgcgatcg tcacccgctc
     5281 gggttcgacc gtcaccgacg ccgagacctc gaccacttcg gtcacgcact tcattgccac
     5341 ccagacgccg gcatagcggc tcatcgcgta tgcatgcagc ccgaggtcga ggtactcctg
     5401 cacggtggcg gggaacagca ccgggatcat gcaggccttg aagatgtggt ccgactggtg
     5461 cggcagcgtc gagctcttgg cgccatgatc gtcgccggcg agcgccacca cgccgccgtg
     5521 cggactggtg ccggccgcat tcgcgtgctt gaacacgtcg ctgcaccgat ccacgcccgg
     5581 ccccttgccg taccacatgc cgaagacgcc gtcgtagcgc gcgccgccaa acaggttcac
     5641 ctgctgcgtg ccccagacgc tggtggcggc caggtcctcg ttcacgccgg gctggaactt
     5701 gatgtggtgc gtctcgaggt gtttcttcgc gcgccacgcg gtctgatcca cgccgcccaa
     5761 cggtgagccg cggtaacccg tgagaaaccc tgcggtgttc agaccggcgg cgagatcgcg
     5821 ctgcctttgc agcatcagta gccgcaccag cgcctgcgtg ccgctcatgt aggcgcggcc
     5881 gcgctcgagc gtccatttgt cgtcaagggt gacctccgcc agggcgcggc ggacggcttc
     5941 gggcagcggt gcgttcatgc gaggacctta tccaaaaatc gtgtttctgg ggtcgcggcg
     6001 gagcacgcga ttgcgcgtcg gccgggccgg aggacgcgcg ccttgtgggc gcggaactcg
     6061 ttcgggattt gcgagggggc gaagcttaac ggctggccgc ggtgcgctca atcgcgcccc
     6121 ccgcggcgtg cgggtctatc ccggcggcag cgcgcgcccg tccttcagcg cgagccagcc
     6181 tcgcgcgatg cggtagatcg cccagatgcc gagcgcgaac aggcccacca cccacagcgc
     6241 gatgccgatc agcacgacgg cgagcagcac cgagagcacc gcgacgacca ccgcccacag
     6301 cagcgcgaac cagaaggtgc ggatctgcca gcggaagtgc gattcgagcc aggtgcctcg
     6361 agcttcgccg cgcttcacgt agttgatgat gaccgcgatg atcgacggcc agccgaatac
     6421 gaacgcgccg acgatggtcg cggcgccgaa cgcgccgatc accagcgaca gcgtgtgcag
     6481 cgcgtacacg atgtgcgcga cgcgcacgag tccgggttca ggggcgacgg cggagggagt
     6541 ggcattcatg atcggctcct gcggcggtgg agggtgcatc gcgttcaagg tgaggcggcg
     6601 cgcgcgcatt tcaagcgacg caggcgcgtc acctggaccg gcgacgctga cgaagtttgc
     6661 gcgccgggac ttctgcgggc ggccgcgaca aggcagcgcc gcgcgcggca caggttgcgt
     6721 ccacctcctc gcgcacgatc tgcgccatgc ggcgcgtccc cggaccggcg gcatcgccgt
     6781 cggcgacgat caggtaaagg gtcacgtagc gctccgcacc ttcgcgcaac ggcagcagtt
     6841 tgagcgcgcc gcgctccagt tcgcctcgga tcgtctcttc gggaaaccac gcgaagccca
     6901 gccccatgcc ggcggcgaag atcgacgtgg ccttgttgct gaacgtccag cgctgttccg
     6961 cgccgcgcca gcctcctcct tcgcgcgtca tcgtcaggcc tgagtcgcgc acgatcagat
     7021 ggcggtgcgt gcgcaggtcg cgataggtca gcggccgccc gagccggtgc agcggatggt
     7081 ccggatgcgc ggcggcgatg aagcgcatct gcatcagcgt gtcgccgacc agtcccggcg
     7141 gcacgctggt gttgatgccg aggtgcacgc ggcgctcgac cagcgcctcg tcggtgccgc
     7201 cgagcacggt ctcgtagagc tgaatgcgcg tttccggccg ctcttcggcg aaacgcgcga
     7261 acgcgcgcag cagcagccag gtcggaaaca cgatgtcggc ggcgatgcgc aactcgggct
     7321 cccagccggc ggcgagcgtg cgtgcggaac gctcgatcgc ctcggcctcg tcgagcagcg
     7381 cacgcgcacg gcgatagagc acttcgccgt gtgcggtcag atgtgccttg cggccttcga
     7441 ccgtgaacag cttcacgtcg agcaggcgct cgatcttctg gatcaggtag ctgacactcg
     7501 actgcgtctt gtgcagccgc gcggatgcct gcgcgtagcc gccggcctcg actaccgcgg
     7561 cgagcgcgcg ccactggtcg agcgagatgc gcgggtggac ggcggcgggc ggcaactgat
     7621 cgaacgattc gaacgaagcg cgcgaaattt tccgcttttc gaccgcggcg aacaatcgtt
     7681 caatggcgcc aacacaacct gcctgatcgg aatcgccatg aagaccctgc tgcagatcaa
     7741 caccagcctg ttcggtgccg gcggcaactc gacgcagctc gccgacaact tcgtcgccgc
     7801 ctggaaggcc ctcaacccag gcggccgggt gatcgtgcgc gacctcgcgc gcgatccgat
     7861 cccgcacttg gccgccgagc acgtgcaggc gttcggtatg cccgccgcgc agcgcaccgc
     7921 cgtgcaggcg accatcgtcg cgcgctcgaa cgcgctgatc gaagaactga agtcggccca
     7981 tgtcgtcgtg ctcgggctgc cgatgtacaa cttcggcgtg ccgtcggttc tgaagtcgta
     8041 cttcgaccag atcgcgcgcg cgggcgtcac gttccgctac acggccaacg gcccggaggg
     8101 cctgatcggc gatcgcccga cctacgtgtt cgccgcgcgc ggcggcatgc accgcgacac
     8161 gccgcgcgac ctgcagaccg gcttcgtcac gacattcctg aatttcatcg gcattcgcag
     8221 catcgagttc gtctatgccg aaggcctcgg ctacggcgac gaagcgaaaa cgaagtcgct
     8281 gtcggctgcg cggcggcaga tcgcggagat cgtcggcgcg gtggagcttg cggccgcgta
     8341 gctacacctg gagatagcgc acccgccgct ccacgccctg ctcgatcagg ccgggcgtgg
     8401 cggcgaagca ccagcgcaca tagccctcgc cctcggggcc gaacgcgatg ccgggcgcga
     8461 gcccgaggcc gaccgttgtc accagcgact tgcagaacgc cagcgaatcg ccggagcggc
     8521 cgggaatccg gaagaacagg tacatcgatc cgggcggcga cgcggcctcg acgcccttga
     8581 gggcgcgcag ccggcccacc aggaggtcgc gtgcgccttt cagccgcgcc accgtctgcg
     8641 cgatcagttc gtcgccgtcg cggatcgcca cgatgccagc gcgctgtacg aaggtcggcg
     8701 cgcacgaggt gttgaattcg accatcttgg cgaagttgga gatgaaggag cgcggtgcca
     8761 ccagccagcc aagtcgccag ccggtcatcg tccacgactt ggagaaggtg ttggcgctta
     8821 tgaagcgttc gtcgggatcg gcctgcgcca acacgccggg tgcgtgcgcc gagccgtcga
     8881 agatgatgcg ttcgtacgca tcgtccgcga cgagccagat gccgtggcgg cgacagtgcg
     8941 ccagcagcgc gttccactgc tcctgcggca tgacccagcc ggtcggattg ccgggcgagt
     9001 tcaccaccac catcctcgtg cccgcaggcg ctgcatcgat gagccgctgc aggtcgagcg
     9061 tccacgtgcc gttggcgcac gtcagcggca cgcgcgccac gcggccgccg acgatctgcg
     9121 gctgcgcggt cacgttcggc cacaccggtg tcacgatcgc gacacgatcg cccggctcga
     9181 tgagcgcctg tgacacgagc gacagcgcgt tgacgccagc gctggtgatc gcgatgcgat
     9241 cgacgccgat cggctcgcgg ggtgcatgca gccggtcgac gtagcctgcc accgcctcgc
     9301 gcagctcgat gatgccgagg ttgtggttgt agaacgtctc gccggcgtcg atcgacgcct
     9361 tggcggcgtt gcggatgaac tcgggcgtgg gcagatcgcc ttcgccgaac cagaacttga
     9421 ccaggtcggc gcggccgatc gcggtgttgg cgacctcgcg gatgcgcgag gcggggagtg
     9481 cgtcgagcga agggcgcagc attgcgcaat tatcgcgcgc cgcgtcacgc acattggcat
     9541 catccgcccc gtgtcgcctt ccggcgcggc ccgagccatg tacccttgcg gctcgcaacc
     9601 gaactgcaag cgcgcatctc atggcaggcc gattcaaacc cgttgccttc gaacccctcg
     9661 gcgggcggcg gtcgcgcatt cgcgtgccgc gctggctcgt gctgctgctg agcggcatcg
     9721 cgctcggcgt ggggggcgtg gtcgtggtgc aggagcgcta tttgccgccg cgcctgacgg
     9781 cggacgagtc gtcgcagctg cgcgccgcgt tcgaacaggc cgataccgag cggcagcggc
     9841 tggccgcgga tctcgcggaa acttcgaagc gtctcgacaa ggcactggcc gacggcaagg
     9901 gtctgagcga ccagcttgcc gccagccggg cgacgaccga ccgactgcgc gacgacctcg
     9961 cgacggtggt cgcggcgttg ccggccgatc cgcgcggcgg cacggtcgag gtgcgcgcgg
    10021 ggcgattcgc tctgcagcgc ggcgcgcttg cctacgacgt ggtgctgacg cgcgagcgcg
    10081 cggccggcaa gccgatgaag ggggtgctgc aactcaccgt ggccggggaa tcggggcgcg
    10141 gcggcgaaag cacggtcgcg ctgagcccga tcaacgtgct gatcggcagc cacgaggtcg
    10201 tgcgcggcag cgcgccgctg ccggagggct tcaggccgcg ccagacgaca gttcaggtgc
    10261 tcgatcgcgt cgcgggcaat tcgctcggca tgcgcgtcat gctggtcaag taggcgccgt
    10321 cccgttctgc gcccgtgccc agcgcagcag cgcggcctcg agccgcgggt gcgcgagaca
    10381 gcgagcgatg aagtcgcgtt cgctacgccc gcccttggcg cggatcacgt ccgccagcgc
    10441 gaggcgctcc gcttcccgcc acgcatcgac ctccagcagc gcgaggatcg gggccatgcg
    10501 ccgccaggct tcgcgctggt caggcgtcag ggcgcgcagc gatgccagtc cgcactggcg
    10561 catcagcgct tcgctcgcct gcgcgacgcc gcgctcacgg tccgatcccg cgcgccgcga
    10621 cagcgctacc cccgccagca atccgagcgc gccgagcgag agcagcggcc gcggatgcgc
    10681 ggaatcgaga tcgaagaaaa ggtgccgcgc ggccagccgc aggagcgtcg cgcgtcgcgt
    10741 gcggtggcgc ggatcgcgtc gcaggcggtc gcgctccacg cgcgtgagcg cacgcgttgc
    10801 ggccgcgcgt ggggcgaagc cgagcttggc gtagaaccac caggcgccgg attcgagtgc
    10861 ctcgtcgttg tccttgccga gttggtacgg ttcgatgctg aacgacgtcg cgccgaacgc
    10921 gtgccgcaag gcagccagcc agcgcgcgaa gacgaacgct gcttcgccgc cgcgaaagga
    10981 ttcgaacgtg ttgaacgaca gcgccgcgcc agcgccaacg tgatccgact gcatgtagcc
    11041 gatcggcaca ccattacgca gcgtcaggct gccatagcag gaggcgagcg cctggcgccg
    11101 ctcgggcaat acgccgatga acgcgaatgc cagtccgtcg ccatcgtcga cgagccaggc
    11161 atcgcgcgcg tcggcgaacg agaacgcctc cagagatcgc gcgcgcgtga ccatcgcgcc
    11221 gcgtgcgaga tcgatgactt cgcggcccgc gcgcggcgac agccgacgca ccgagcgcgg
    11281 cgcgcgcagc agttcggcgc gcaggtccgg ccgcacgcgc ggtggcggcg cgcgccggaa
    11341 cacgaccggc gcgccgcgca actccgcccg ggtgcgcgat ggcgtatcgg gaccgggcgc
    11401 gagcacgaaa ctcgcgtcga tcgcatccgc aaacgcttcg cgcgtaaatg tgttgcctgg
    11461 catcgcggcg atccgccgca ggaggaacgc ggcgtcggtc tcgtcgccgc gcaaccggtc
    11521 cagcgccgcg tagccggcga gtttcagttc catcaatgcg gtgctctccg ccgacgacag
    11581 cagcggcggc agcgcgcgcg cgatgttggc gtccgcggcg gtatcgctgc ggtcgagccg
    11641 cagccatgcc ggccacttcg cggcgagcca ttgcgcctgg ccggcgaaga agcggtagtg
    11701 gatcggcgtg ccggcgatgc cggtgtcggc gagcgcagcg cgcaccgcac gcaggtcggc
    11761 gcgcccggcg aagccggcca gcatcgcctg cacctgcgcg agcactgccg cgtcatcggg
    11821 gtaggcacgg atgaagcaca gtgcctcgtg cagccgcttc accgcgcgcg cgctgccgag
    11881 ccggatgcgg tcgaggcgcc gcagcaacgc gagcttggca tccgccgccg cggggccgaa
    11941 ctgcaggcgg caacgctcga gcgcggcgag cgggggcgag ggcatggccg cgcatggtac
    12001 gcggccgcgc ccttgcgcta gggaaacact gaacaagctc tcgcagagtt tgtcgtagtc
    12061 ggcaatgctt g
//
LOCUS       DBNHBY010000212         2091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_620470, whole genome shotgun sequence.
ACCESSION   DBNHBY010000212 DBNHBY010000000
VERSION     DBNHBY010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_620470"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16HB_05205"
     CDS             complement(<1..208)
                     /locus_tag="AC16HB_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5606222.1"
                     /translation="MAKAGQDRAAEMTALVRAVDGGGFSQAARALGVTPSAISKTITR
                     LEARLGVRLLNRTTRRLTPTPEGEA"
     gene            207..545
                     /locus_tag="AC16HB_05210"
     CDS             207..545
                     /locus_tag="AC16HB_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606223.1"
                     /translation="MISQILCQSAGLYAFKSQITLAFIGRPSAVAERESAMDHDFGPN
                     RTIDYDFHKARAHELRQQEIDRLFERVVAWARSLTGTHTDASRRLRSGNAARRQSPKL
                     GMRGASDAHC"
     gene            588..>2091
                     /gene="groL"
                     /locus_tag="AC16HB_05215"
     CDS             588..>2091
                     /gene="groL"
                     /locus_tag="AC16HB_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238928.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5606224.1"
                     /translation="MAAKQILFYDDARERIRDGLNQLADAVKVTLGPKGRTVVLGRSF
                     GAPTVINSGVAVAREIELEDPVENLGAQMVREVASKTSELAGDGTTTATVLAQSMVNE
                     GAKFVAAGHDPMEIKRGIDLAVERVVERLKTNSRPCSAKQEIAQVGTISSNGDESIGA
                     LIADAMERVGESGVIKSEDARGMTSELEVVEGLQFDRGYLSPYFINDGEKQRVVLDDA
                     YVLIYEKTISSVRDLLPLLEEVIKQNKPLLVIAEDVSGEALATLVVNAIRGVLKVCAV
                     KAPGFGDRRTAMLQDIAIVTGGALVSETTGRTLEKAALEDLGRARRIEIDKDNTTLIG
                     SAGDAAQIAARIAGLKKEVEKTTSDYDREKLEERIAKLSGGVALIRVGASTELEMKEK
                     KSRVEDALHATRAAKAEGIGPGGGVALIRAGAALADLPGKSLSQQAGIDIVKRALEEP
                     LRQLLRNAGLEPAPIVDRVKSESGSFGYNVAAEQYGDLVAMGVLDPTKVTR"
BASE COUNT          370 a          686 c          739 g          296 t
ORIGIN      
        1 acgcctcgcc ttcgggagtg ggtgtcagcc ggcgcgtggt gcgattgagc agccgcacgc
       61 cgagccgcgc ctccaggcgc gtgatcgtct tcgagatcgc ggacggcgtg acgccgagtg
      121 cgcgtgcggc ctgcgagaag ccgccgccgt cgactgccct cacgagcgcg gtcatctcgg
      181 ccgcgcggtc ctgccccgcc tttgccatga tttcacaaat cctgtgccag agcgccggat
      241 tgtatgcttt caagtcgcaa attacattgg cgttcatcgg cagaccgtct gccgttgcag
      301 aaagagagag cgccatggac cacgacttcg gaccgaatcg aacgatcgac tacgacttcc
      361 acaaggcgcg cgcgcacgaa ctgcggcagc aggagatcga ccggctgttc gagcgcgtcg
      421 tggcatgggc tcgctcgctg accggcacgc atacggacgc gtcacggcgc ttgcgctcgg
      481 gcaatgctgc gcggcgccag tctcctaagc tcggaatgcg tggggcgtcc gacgcgcact
      541 gctgaagtgc ccgccgcacc ccgcgcgcaa tcgagcgggg tgcacagatg gcggccaagc
      601 agatcctctt ctacgacgac gcacgcgagc gcattcgcga cgggctgaac caactcgccg
      661 acgcggtcaa ggtcaccctc ggaccgaaag gccgcacggt ggtgctgggc cgctcgttcg
      721 gcgcgccgac ggtgatcaat tccggcgtgg cggtcgcgcg cgagatcgag ctcgaagatc
      781 cggtagagaa cctgggcgcg cagatggtgc gcgaggtcgc ctcgaagacc tccgagctgg
      841 cgggcgacgg gacgaccacc gcgaccgtgc tggcgcagtc gatggtcaac gagggcgcca
      901 agttcgtcgc cgccggccac gacccgatgg agatcaagcg cggcatcgat ctggcggtcg
      961 agcgcgtggt cgagcggctc aagaccaact cgcgcccgtg ctccgcgaag caggagatcg
     1021 cccaggtcgg cacgatttcc agcaacggcg acgagtcgat cggcgcgctg atcgccgacg
     1081 cgatggaacg cgtgggcgaa tccggcgtga tcaagtccga ggacgcgcgc ggcatgacct
     1141 ccgagctcga ggtggtcgaa gggctgcagt tcgaccgcgg ctatctgtcg ccgtatttca
     1201 tcaacgacgg cgagaagcag cgcgtggtgc tcgacgatgc ctacgtgctg atctacgaga
     1261 agacgatctc gtcggtgcgc gacctgctgc cgctgctcga ggaggtgatc aagcagaaca
     1321 agccgctgct cgtgattgcc gaggacgtga gcggcgaggc gctcgcgacc ctggtcgtca
     1381 acgcgatccg cggcgtgctg aaggtgtgcg cggtcaaggc gccgggtttc ggcgaccgcc
     1441 gcaccgcgat gctgcaggac atcgcgatcg tcaccggcgg cgcgctggtg tccgaaacca
     1501 ccggccgcac gctggagaag gccgcgctcg aggatctcgg ccgcgcacgg cgcatcgaga
     1561 tcgacaagga caacaccacg ctgatcggct cggccggcga tgcggcgcag atcgccgcgc
     1621 gcatcgcggg gctgaagaag gaagtcgaga agaccaccag cgactacgac cgcgagaagc
     1681 tcgaggagcg catcgcgaag ctttccggcg gcgtggcgct gatccgcgtc ggcgcctcca
     1741 ccgagctcga aatgaaggag aagaagtcgc gcgtcgagga cgcgctgcac gccacgcgcg
     1801 cggccaaggc cgaaggcatc ggccccggtg gcggcgtggc gctgatccgg gccggcgccg
     1861 cgctcgcgga tctgccgggc aagtcgctgt cgcagcaggc cggcatcgac atcgtcaagc
     1921 gcgcgctcga ggagccgctg cggcagctgc tgcgcaacgc cggactggag ccggcgccga
     1981 tcgtcgatcg cgtgaagtcc gaaagcggca gcttcggcta caacgtcgcc gccgagcagt
     2041 acggcgatct cgtcgcgatg ggcgtgctcg acccgaccaa ggtcacgcga c
//
LOCUS       DBNHBY010000213         2817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_350605, whole genome shotgun sequence.
ACCESSION   DBNHBY010000213 DBNHBY010000000
VERSION     DBNHBY010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_350605"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      112..221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            267..1355
                     /gene="gcvT"
                     /locus_tag="AC16HB_05220"
     CDS             267..1355
                     /gene="gcvT"
                     /locus_tag="AC16HB_05220"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5606225.1"
                     /translation="MTLKRTPLFETHVASGARMVDFGGWEMPVNYGSQIEEHHAVRRR
                     AGMFDVSHMRVVDVDGAASRDFLRTLVANDVVKLTPGKALYTCMLNDAGGVLDDLIIY
                     WIHTDRYRIVVNAATADKDIAWMQQVAAARRFNVNLRPRPDLAMIAVQGPQARAILAQ
                     VLPEAAELIGGLKVFSAAFRDDWFIARTGYTGEDGCEIVLPATEAAAFWRRLKAAGVA
                     ECGLGARDTLRLEAGMNLYGQDMDETVSPLESGLAWTVSLAEPRDFIGRGALENQQVR
                     GGLRQLVGLKMLDKGVLRAHQKVMTPHGDGEITSGTFSPTLGFSIALARVPGGVQVGD
                     RIQVDLRGKPVAAQVVKYPFVRNGKALI"
     gene            1382..1768
                     /gene="gcvH"
                     /locus_tag="AC16HB_05225"
     CDS             1382..1768
                     /gene="gcvH"
                     /locus_tag="AC16HB_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5606226.1"
                     /translation="MKIPADLRYTESHEWVKSEADGTVTVGITDFAQDALGDLVFVEL
                     PEVGRVLKAGEACAVVESVKAASDVYAPVAGTVVAAHDALKDKPEAINSDAFGAWLFK
                     LKPANAGDLAKLLDATKYQANIEAAK"
     gene            1769..>2817
                     /locus_tag="AC16HB_05230"
     CDS             1769..>2817
                     /locus_tag="AC16HB_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HIR5606227.1"
                     /translation="MTTIAQLEHHDAFVARHIGPNDEEIALMLAVIGQPSLEAMVDAI
                     VPASIRLAAPLALPGATTEEEALAKLAAIAQKNQVFRSFIGMGYYGTFTPNVILRNIL
                     ENPAWYTAYTPYQAEISQGRMEALINFQTMVCDLTGMEIANASLLDEGTAAAEAMTLA
                     KRSCKSKSNVFFVSADCHPQTIAVVRTRAEALGIEVTVGDDAAAAKADAFGVLLQYPC
                     STGDVRDYADIVRAAHARGALVAAAADLLALTLIEPPGEWGADIVVGNSQRFGVPFGF
                     GGPHAAFLACRDAFKRSMPGRLVGVSVDAQGQPAYRLTLQTREQHIRREKATSNICTA
                     QVLLAVMASMYAVYHG"
BASE COUNT          490 a          922 c          991 g          414 t
ORIGIN      
        1 gcggtgtatc cttccgcatc gctcaacgcg cgacacggga gagcgctccg aaccgcggag
       61 ccgccgaagg cgcaacgccc gtaatcgctc aggtaccgaa aaccgcctcg cgcgagcgac
      121 tctggagaga ccggccctgc atctgccagc acgggacggc gccgaagggg caggggccga
      181 accggttcca aactctcagg caaaaggaca gaggggcgcc cgtcgattcc cgacgggtgc
      241 cccatttgtt tttttggagg ctccggatga cgctcaaacg cacgccgctc ttcgaaaccc
      301 acgtcgcctc cggcgccagg atggtcgact tcggcggctg ggaaatgccg gtcaactacg
      361 gctcgcagat cgaggagcac cacgcggtgc ggcggcgcgc ggggatgttc gacgtgtcgc
      421 acatgcgcgt cgtcgacgtc gatggcgcgg cgtcgcgcga cttcctgcgc accctggtcg
      481 ccaacgacgt ggtcaagctc acgcccggca aggcgctgta cacctgcatg ctgaacgacg
      541 ccggcggcgt gctcgacgat ctgatcatct actggatcca caccgaccgc taccggatcg
      601 tcgtcaacgc ggcgactgcc gacaaggaca tcgcgtggat gcagcaggtc gcggcggcgc
      661 gcaggttcaa cgtgaacctc agaccgcggc ccgatctggc gatgatcgcg gtgcagggcc
      721 cgcaggcgcg cgcgatcctg gcgcaggtgc tgccggaagc ggccgaactg atcggcggct
      781 tgaaggtgtt cagcgccgca ttccgcgacg actggttcat cgcgcgcacc gggtacacgg
      841 gcgaggacgg ctgcgagatc gtgctgcccg cgaccgaggc ggccgcgttc tggcggcggc
      901 tgaaggcggc cggtgtcgcc gaatgcggac tcggcgcacg cgacacgctg cggctcgagg
      961 ccggcatgaa cctgtacggg caggacatgg acgagaccgt gtcgccgctg gaatccgggc
     1021 tcgcgtggac cgtcagcctg gcggagccac gcgacttcat cggccgcggc gcgctggaaa
     1081 accagcaggt gcgcggcggg ctgcggcaac tggtcggcct gaagatgctc gacaagggcg
     1141 tgctgcgcgc gcatcagaag gtgatgacgc cgcacggtga cggcgagatc accagcggca
     1201 ccttttcgcc cacgctcggc ttctcgatcg cgctggcacg cgttccggga ggcgtgcagg
     1261 tcggcgaccg gattcaggtg gatctgcgcg gcaagccggt ggcggcgcag gtcgtcaagt
     1321 atccgttcgt tcgcaacggc aaggcgctga tctgaatctc acgaatcacg aaaggaagaa
     1381 catgaagatt cccgcagacc tcaggtacac cgaatcgcac gagtgggtga agagcgaggc
     1441 cgacggcacg gtgacggtcg gcatcacgga tttcgcgcag gacgcgctcg gcgatctggt
     1501 gttcgtcgaa ctgcccgagg tcggccgcgt cttgaaggcc ggtgaagcgt gcgcggtggt
     1561 cgagtcggtg aaagccgcgt ccgacgtgta cgcgccggtg gccggcaccg tcgtcgccgc
     1621 ccacgacgca ctgaaggaca agccggaggc gatcaacagc gacgcgttcg gcgcgtggct
     1681 cttcaagctg aaacccgcga atgcgggcga cctcgccaag ctgctcgacg cgacgaaata
     1741 ccaggccaac atcgaggcgg caaagtagat gacgaccatc gcgcaactgg agcaccacga
     1801 cgcgttcgtc gcgcgccaca tcggccccaa cgacgaggag atcgcgctca tgctggccgt
     1861 gatcggccag ccgtcgctcg aggcgatggt cgatgcgatc gtgcccgcat cgatccggct
     1921 tgccgcgccg ctcgcgctgc cgggcgccac gaccgaggaa gaggcgctgg cgaagctcgc
     1981 ggcgatcgcg cagaagaacc aggtcttccg cagcttcatc ggcatgggct actacggcac
     2041 gttcacgccg aacgtcatcc tgcgcaacat cctggagaac ccggcctggt acaccgcgta
     2101 tacgccctac caggccgaga tctcgcaggg gcggatggaa gcgctgatca acttccagac
     2161 gatggtgtgc gatctcacgg ggatggagat cgccaacgcg tcgctgctcg acgaaggcac
     2221 ggccgcggcg gaagcgatga cgctcgccaa acgctcgtgc aagagcaagt cgaacgtgtt
     2281 cttcgtgtcg gcggactgtc atccgcagac cattgcggtc gtgcgcacgc gcgccgaggc
     2341 gctcgggatc gaggtcacgg tcggcgacga tgccgccgcc gcgaaggccg atgcattcgg
     2401 cgtgctgctg cagtacccgt gctcgaccgg cgacgtgcgc gactacgcgg acatcgtgcg
     2461 cgcggcgcac gcgcgcggcg cgctggtggc cgcggccgcc gatctgctcg cgctgacgct
     2521 gatcgagccg ccgggcgagt ggggtgccga catcgtggtc ggcaactcgc agcgcttcgg
     2581 cgtgccgttc ggattcggcg gcccgcacgc ggcgttcctc gcttgtcgcg acgcgttcaa
     2641 gcgctcgatg ccgggccggc tggtgggggt gtcggtcgac gcccagggcc agcccgcgta
     2701 tcgcctcacg ctgcagacgc gcgagcagca catccggcgc gagaaggcga cctcgaacat
     2761 ctgcaccgcg caggtgctgc tggcggtgat ggcgagcatg tacgccgtct atcacgg
//
LOCUS       DBNHBY010000214        12095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_350659, whole genome shotgun sequence.
ACCESSION   DBNHBY010000214 DBNHBY010000000
VERSION     DBNHBY010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_350659"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="AC16HB_05235"
     CDS             <1..772
                     /locus_tag="AC16HB_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5606228.1"
                     /translation="AGAGGGIGMAAAAKAAPDGYTVLLALSSISIIPEADRILKRPAM
                     YELKQLKPVARFTADPTVLVVRADSPWQTARDFIDAMKREPTRYSYGSSGNYGTMHVP
                     MEMLKNAAGFRLLHIPYTGAGPAIVALLGHQVDALATGPSTIVQYVRSGRLRALAHWG
                     DGRLAALPDVPSLAELGYPVQFSQWSGLFVPAATPEPTVARLREAARAAANDSKVQQT
                     IRTAGSPVLYLDAPEFQSFWDTDAARMVDAVKQIGRVD"
     gene            complement(780..1286)
                     /locus_tag="AC16HB_05240"
     CDS             complement(780..1286)
                     /locus_tag="AC16HB_05240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606229.1"
                     /translation="MRIRIADVDDAEDIARIHVLTWQSAYRGLVCDDFLDSQSVERRR
                     ALRRERIEQAPGSVLIAADGAACGWIAFGRAPDVDAPPATGEVWALDVLPAAWCRGTA
                     RALWSAARERLIGDGMKPATLWVFSNNVRARAAYERLGWQPEPGSERTFELGGRTMRE
                     TRFRIALA"
     gene            1354..2817
                     /locus_tag="AC16HB_05245"
     CDS             1354..2817
                     /locus_tag="AC16HB_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR5606230.1"
                     /translation="MLLAAVDLGSNSFRLEIGRADGNRVEPLGYWKETVRLARGIGDD
                     GRLSKRSIDTACECLARMNERLRGLKNEQVRAVGTQTLREAVNVNEFLLEAQAALGHP
                     IEVISGREEARLTFEGCMHALPPSAQPRLVIDIGGASTELIVGRGFTAEAAESFKIGC
                     VSVTHRFFTDGEIDRSRFKRAVVACAAELEEAIAQFARGRWDEAYGTSGTIGAVSEIL
                     RSEGWASGTITSEGLQRLRQALIDFGAVKRIRLNGIRPDRQEVIAGGVAVLLAVFETL
                     RIGEMRPGRGGLRTGVLYDLLGRRERRDLRDATVERLQSRFGVDRAQAQQVARIAGGF
                     HAALEPDADEEMAKRVHWAAALHEIGFAISHNDHHKHGAYLVRHGDLPGFSTTDQERI
                     ATLVLAQRGNLKKVIDALMEEPRVAKIVALRLAIILCHARREVQLPRWRLTTGKTWIE
                     LALAAEWLADHPLTQHLLEEEAAQWAKVGIRFSLKPL"
     gene            complement(2830..3294)
                     /gene="sixA"
                     /locus_tag="AC16HB_05250"
     CDS             complement(2830..3294)
                     /gene="sixA"
                     /locus_tag="AC16HB_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR5606231.1"
                     /translation="MELILWRHAEADAGEPDLGRKLTAKGEKQARRVAEWLHAQLPDS
                     ARVLVSPAARAQQTAQALADLSQRRLKTVVELSPGASADAVLSAAGWPDGNSTVVIVG
                     HQPTLGRVASRLLAGRELGWSMRKGAVWWFSARERAGDEQVVLRAVVSPDLL"
     gene            complement(3304..5472)
                     /gene="ppk1"
                     /locus_tag="AC16HB_05255"
     CDS             complement(3304..5472)
                     /gene="ppk1"
                     /locus_tag="AC16HB_05255"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR5606232.1"
                     /translation="MRWKHRIRHRLGQGRKDSKIRPVAAPPERYFNRELSQLAFNERV
                     LALAERGDVPLAERLRFVCIVSSNLDEFFEIRVSGLIDEMRDDVGVGGGWGPQASQWE
                     HYLQISARAQRLVERQYALFNHVLMPRLARRGIVVVSHAERTPAQRGWVREYFESEVR
                     PLLAPIGLDPSHPFPQVMNKALNFIVALEGKDAYGRPAATAILNVPRVLPRVIPLPAR
                     LAPGKQAFAMLTSVIRAHLGDVFPGRKMVGFSQFRVTRDSDLWVDEREVKNLRQAMRV
                     ELTQRHFGNPLRLEVLRTCPPHLELMLREQFALPNEAVFRVEGPVNLVRMNQLVDHVS
                     QPELRWPPFVPAWPAKITPAADGADLFEAMKRGDILLHHPYESFEPVIEFLRQSAVDP
                     RVVAIRMTLYRTGTASVIVDLLVDAARRGKDVTVIIELKARFDEEANINLAERLEAVG
                     AQVVYGVVGLKTHAKMLLVLRREEMPGNRGAAKARLVHYAHLGTGNYHPATSKLYADF
                     GMLTADTALCADMERVFMHLTSQNKLSKLAHLWVAPFTLHKRVLAAIAREATIARSGK
                     PGRIVAKMNALIDEATIDALYAASNAGVKIDLIVRGACSLRPGVKGLSENITVRSIVG
                     RFLEHHRIYYFRNGGEDDVYLSSADWMGRNLFRRIEIAWPVLNRKLKQRVMDEGLLPF
                     LIDTTNAWTLGSAGDYRPPRPRKRSYSAQQALLQRLTAAN"
     gene            5583..6263
                     /locus_tag="AC16HB_05260"
     CDS             5583..6263
                     /locus_tag="AC16HB_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR5606233.1"
                     /translation="MNETLPESPPLIVVERVRKRYGEAAPTIDDVSFTVARGEFLLLT
                     GPSGAGKSTLLRLLAALESPSSGRIVVADTELAKLNQRARALLRRSLGIVPQDLLLLH
                     DRTALENVMLPALIAGLARSEAESRALAALARVDVVAADQYPPALSGGAQQRVALARA
                     IVNRPTAILADEPTAHLDDAAASGIVRLLDEFSAAGVAVVLASHGESVALPARTRVLR
                     LTNGRTAA"
     gene            6260..7168
                     /locus_tag="AC16HB_05265"
     CDS             6260..7168
                     /locus_tag="AC16HB_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR5606234.1"
                     /translation="MSGAARLSHALREAGRLLRRRSATFSLAVLLAACALVLPLLAIT
                     VGYGLAPLANRVPIAPEISVFASLSATGQDIATLKSKLEALPNVERVQWITRDQSLAE
                     LARRSGGLAPLADVKPNPLPDTLVVSLNRRVQPDDLDRAANEIRKLARVDAVYADSSW
                     FRKAVGLTQVVVKVAAFVGIATVGLLALVVVGAVRLIAISDRDELRLLRLIGAEDRQI
                     ARPYAYAGGIALLLASMLATATVAALLQWLMPDLDWLAQVLAVQIGLGPLPWPTLAGL
                     AAVAFLIGMAGGALGLRSALRRIVRR"
     gene            7331..8230
                     /gene="rpoH"
                     /locus_tag="AC16HB_05270"
     CDS             7331..8230
                     /gene="rpoH"
                     /locus_tag="AC16HB_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR5606235.1"
                     /translation="MATSLNALVPVGNSALALHPGSLGNLDAYISAVNRVPLLTPDEE
                     LRLARAFRAEGDLEAAQRLVLSHLRLVVAVARNYLGYGLPHADLIQEGNIGLMKAVKR
                     FDPERGVRLVSFALHWIKAEIHEFILRNWRLVKVATTKAQRKLFFNLRSMKQGIRTMS
                     PDQVAEVASTLNVKPEEVVEMETRLSGGDVALESRAEDGEDEYAPIAYLADSGSEPVQ
                     VLERREYDRLQTDGIRAALEGLDARSRRIIEARWLNEDAQGSVGSATLHDLADEFGVS
                     AERIRQIEAKALQKMRGALAEYA"
     gene            8568..9008
                     /locus_tag="AC16HB_05275"
     CDS             8568..9008
                     /locus_tag="AC16HB_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606236.1"
                     /translation="MHAALLAQLPPGKGLRLRERFEWYACRGAGFHTDAHYGGVLFGA
                     WCVAGPKREIVFSRSGLRVGAAVGELVVFDPFEPHAVLDPGQARYAREHFERAPVSLF
                     LGFEIDLDAAARVAFGIGAPPAGGAILSSAVAVNAETGALPGSR"
     gene            complement(9005..9568)
                     /locus_tag="AC16HB_05280"
     CDS             complement(9005..9568)
                     /locus_tag="AC16HB_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5606237.1"
                     /translation="MRRLLWILGIAVLAACSPPTFNNVDVTGADFARDFTLTDHTGAR
                     RTLADYRGKVVAIFFGFTQCPDICPTTLADMAQVKKKLGTDGDRLQVLFITVDPARDS
                     REVLAQYVPGFDPSFVGLYGTDEEIARTAKEYKVFYQKVAGKTPTSYTIDHTAGTFVY
                     DKEGRLRLFLKHGGDIDAIVADLKKLL"
     gene            complement(9598..10497)
                     /gene="cyoE"
                     /locus_tag="AC16HB_05285"
     CDS             complement(9598..10497)
                     /gene="cyoE"
                     /locus_tag="AC16HB_05285"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5606238.1"
                     /translation="MQTHTSIEYIAPVAHLARQYWLLTKPRVTQLALFCAVIGMFLST
                     PELPAVGLVLKATAGIWLLAGAAFAVNSIIERHIDARMQRTRMRPVARGEITPAQAMF
                     FSGLIGGAGMWVLYTQVNALTMWLTFATFVGYAVIYTVLLKPYTPQNIVIGGMSGAMP
                     PVLGWAAITNAAPAEAWVLALIIFVWTPPHFWALALYRLDDYRKSGLPMLPVTHGSEL
                     TRLHILLYTIVLVAVTMLPFVIQMAGALYLGAALALGAMFLVYAGRLLRGYSDGLARS
                     TFNYSIVYLAALFAALLIDKYLG"
     gene            complement(10546..11646)
                     /locus_tag="AC16HB_05290"
     CDS             complement(10546..11646)
                     /locus_tag="AC16HB_05290"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5606239.1"
                     /translation="MIQADALLWLALKAIAVAALPLAYVWRSRDPAKLRKLAWVTAFL
                     TLDLVMFGSFTRLTDSGLGCPDWPGCYGHSNPLSAGEPIRAAQTAQPTGPVTMTKAWI
                     EMLHRYFAMAVGFLIIVLMAACWMRWRRDRSHPAPLLITATFLTVCLQGAFGAWTVTM
                     KLQPVIVTTHLLGGVALLALLTWFALRESPSRRVAPRATDIGVHAAAGLIVLALQIAL
                     GGWVSTNYAVLACPDFPLCQGELMPPADFRHGFTLWRDLGMTGDGAPIPFQALVAIHW
                     AHRAFSLAAFVVLGTLAWRAWRLEGLRGPARWLAMLLAAQFLTGLSNVVLQWPLLLAV
                     VHNGSAALLIAIMVVVLYRIGSAGRAAVLDGG"
     gene            complement(11646..>12095)
                     /locus_tag="AC16HB_05295"
     CDS             complement(11646..>12095)
                     /locus_tag="AC16HB_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5606240.1"
                     /translation="VEPQRPMPALALRELDGRDVATSALRGRWLLVTVDSGDCRDACQ
                     RKLWQMRQVRLTTGKDADRVERIWLVDDAAPLPTIVMREFDGTRFLRADPAQLESYLP
                     LPAGGRLTDHIWVIDPLGNLMLRWPKDADPNRMKRDLARLLKASGIG"
BASE COUNT         2077 a         3987 c         4047 g         1984 t
ORIGIN      
        1 cgccggcgcg ggcggtggta tcggtatggc ggccgcggcc aaggccgcac ccgacgggta
       61 cacggtgctg ctcgcgctgt cgtcgatctc gatcatcccc gaggctgatc ggatactgaa
      121 gcggccggcg atgtacgagt tgaagcagct gaaaccagtc gcgcgcttca ccgctgaccc
      181 gaccgttctg gtcgtgcgcg ccgacagccc gtggcagacc gcgcgcgatt tcatcgacgc
      241 gatgaaacgc gagccaaccc ggtattcgta tggctcgtca ggcaattacg gcacgatgca
      301 tgtgccgatg gagatgctga agaacgccgc aggcttccgc ctgctgcaca tcccttacac
      361 cggcgcgggg cccgcgatcg tcgcgctgct cggtcaccag gtcgacgcgc tggcgacggg
      421 tccgtcgacg atcgtgcagt acgtccgcag tggacggctg cgtgcgctcg ctcactgggg
      481 cgatgggcgg ctggccgcgc tgcccgacgt gccgagcctc gccgagctcg gctatccggt
      541 acagttctcg cagtggtcgg ggctgttcgt tccggccgcg acgcccgagc ccacggtcgc
      601 gcggttgcgc gaggcggcgc gggcggccgc gaacgactcc aaggtgcagc agaccatccg
      661 aaccgcgggc agccccgtcc tgtatctcga cgcgcccgag ttccagagct tctgggacac
      721 cgatgccgct cgtatggtcg acgcggtgaa acagatcggc cgcgtcgact gaagtgcgtt
      781 catgccaggg cgatgcggaa acgggtctcc cgcatcgtcc ggccgccaag ctcgaacgtt
      841 ctttcggaac cgggttccgg ttgccatccc agacgttcgt aggcggcacg tgcacggacg
      901 ttgttggaaa agacccacag cgtggccggt ttcatgccgt cgccgatcag ccgctcgcgc
      961 gccgcggacc acagcgcgcg agctgtaccg cgacaccagg cggccggcag aacatccaac
     1021 gcccatactt cgccggtcgc gggtggtgcg tcgacgtcgg gcgcgcggcc gaaggcgatc
     1081 cagccgcatg ctgcgccgtc cgcggcgatc agcacgctgc caggcgcctg ttcgatgcgc
     1141 tcgcgccgca acgcacgacg ccgctcgacc gactgcgagt cgaggaagtc atcgcacacg
     1201 agaccgcgat atgcgctttg ccacgtgagc acatggatgc gcgcgatgtc ctcagcgtcg
     1261 tcaacgtctg caatccggat tctcatcgcg tcgcagtccg gtccttagaa tcggcgcagt
     1321 ctattcgacc gtcctctcgg ggcactccgc cgcatgctgc tcgccgccgt cgatcttgga
     1381 tcgaacagct ttcggctgga gatcgggcgc gccgacggca atcgcgtcga gccgctcggc
     1441 tattggaagg agacggtgcg cctggcgcgc ggcatcggcg acgatgggcg gctgtcgaaa
     1501 aggtcgatcg acaccgcgtg cgaatgcctg gcgcgcatga acgagcggtt gcgcgggctg
     1561 aagaacgagc aggtgcgcgc ggtcggcacg cagacgctgc gcgaggcagt caacgtgaac
     1621 gagttcctgc tcgaggcaca ggccgcgctc ggccatccga tcgaagtgat ctcgggccgc
     1681 gaagaggcgc gcctcacctt cgaaggttgc atgcacgcgc tgccgccgtc ggcgcagccg
     1741 cgcctcgtga tcgacatcgg cggcgcgtcg actgaattga tcgtcggccg cggcttcacg
     1801 gccgaggctg cggaatcgtt caagatcggc tgcgtaagcg tcacgcaccg cttcttcacg
     1861 gacggcgaaa tcgaccgcag ccgattcaaa cgcgcggtgg tcgcgtgcgc ggccgaactg
     1921 gaggaggcga tcgcacagtt tgcgcgcggc cgctgggacg aggcatacgg gaccagcggc
     1981 acgatcggtg ccgtgagcga gatcctgcgc agcgaaggct gggcgagcgg cacgatcacg
     2041 agcgaaggcc tgcaacggct gcggcaggcg ctgatcgatt tcggcgcggt caaacgtatc
     2101 cgcctgaacg gcatcagacc agaccggcag gaggtgatcg ccggcggggt cgcggtgctg
     2161 cttgcggtgt tcgagacgct gcgcatcggc gagatgcggc cgggccgcgg cgggctgcgc
     2221 accggcgtcc tgtacgacct gctcggccgg cgcgagcggc gcgatctgcg cgacgccacg
     2281 gtcgagcgcc tgcagtcgcg ttttggcgtc gatcgcgcac aggcgcagca ggtcgcgcgc
     2341 atcgccggcg ggttccacgc ggcgctggag ccggacgctg acgaggagat ggcgaagcgc
     2401 gtgcattggg cagccgcgct gcacgagatc ggatttgcca tctcgcacaa cgaccatcac
     2461 aagcacggcg cgtatctggt ccgccatggc gacctgcccg gcttctcgac caccgatcag
     2521 gagcgcatcg cgacgctggt cctcgcgcag cgcggcaacc tgaagaaggt gatcgacgcg
     2581 ctgatggagg agccccgcgt ggcgaagatc gtcgcgctgc ggctggcgat catcctgtgc
     2641 cacgcgcggc gtgaggtgca gttgccccga tggcggctca cgaccggcaa gacgtggatc
     2701 gaactcgcgc tcgcggccga gtggctcgcc gatcacccgc tgacgcagca cctgctggaa
     2761 gaggaagctg cgcagtgggc caaggtcggc atccggttca gcctgaagcc gctctagcgc
     2821 acccggcagt cagaggaggt cggggctgac caccgcgcgc agcaccactt gttcgtcccc
     2881 ggcgcgctcg cgcgcgctga accaccacac tgcccccttg cgcatcgacc agccgagttc
     2941 gcgccccgcg agcaggcgac ttgcgacccg acccagcgtc ggctgatgac caacgatcac
     3001 gacggtcgaa ttgccgtcgg gccagccggc cgccgagaga acggcatctg cgctcgcgcc
     3061 gggactcagt tcgacgaccg tcttcagcct tcgctgcgac aggtccgcga gggcctgcgc
     3121 ggtctgctgc gcccgcgcag ccggactcac cagcacgcgc gcggaatccg gcaactgtgc
     3181 gtgcagccat tcggcgacac ggcgcgcctg cttctcgccc tttgcggtca gcttgcgtcc
     3241 gaggtcgggc tcgccggcat cggcttccgc gtggcgccac aggatcagtt ccattctcct
     3301 tgctcagttg gctgcggtca gccgttgcag cagcgcctgc tgcgccgagt aactgcgctt
     3361 gcgcggcctc ggcggccgat agtctccggc ggaaccaagc gtccatgcat tggtcgtgtc
     3421 gatcaggaac ggcagcagtc cttcgtccat gactcgctgc ttgagcttcc ggttcagcac
     3481 cggccaggcg atctcgatac gacgaaacag gttgcgcccc atccagtctg cgctcgaaag
     3541 atagacatca tcctcgccgc cgttgcggaa gtagtagatg cggtgatgtt ccagaaagcg
     3601 gccgacaatc gagcgcacgg tgatgttctc cgacagcccc ttcacgcccg gacgcaggct
     3661 gcacgcgccg cgtacgatca ggtcgatctt cacgccggcg ttcgaggccg cgtacagcgc
     3721 atcgatggtc gcctcgtcga tcagcgcgtt catcttggcg acgatccggc ccggcttgcc
     3781 gctgcgggcg atcgtcgcct cgcgcgcgat cgcggccagc acgcgcttgt gcagcgtgaa
     3841 cggcgcgacc cacaggtgcg cgagcttcga cagtttgttc tggctggtga ggtgcatgaa
     3901 cacgcgctcc atgtcggcgc acagcgcggt atcggcggtc agcatgccga aatcggcata
     3961 cagcttggac gtggccgggt ggtagttgcc ggtgcctagg tgcgcatagt ggaccagccg
     4021 cgcctttgcc gcaccccggt tgccgggcat ttcctcgcgg cgcaggacga gcagcatctt
     4081 ggcgtgcgtc ttcaggccta cgacgccata gacgacctgc gctccgaccg cctccagtcg
     4141 ttcggctagg ttgatattgg cttcctcgtc gaagcgcgcc ttcagctcga tgatcacggt
     4201 gacgtccttg ccgcgccgcg cggcatcgac cagcaggtcg acgatcaccg aagcggtgcc
     4261 ggtgcgatac agcgtcatgc ggatcgcgac caccctcggg tcgacggcgg actggcgcag
     4321 gaactcgatc accggctcga agctctcgta cgggtgatgg agcaggatgt cgccgcgctt
     4381 catcgcctcg aacagatcag cgccgtcggc tgccggcgtg atcttggccg gccaagccgg
     4441 tacgaagggc ggccagcgca attccggctg cgatacgtga tcgaccagct ggttcatacg
     4501 caccaggttt accggacctt cgacgcggaa cacggcctcg ttcggcagag caaactgctc
     4561 gcgcagcatc agttccaggt gtggcggaca ggtgcgcagc acctcgagcc gcagcggatt
     4621 gccgaagtgg cgctgcgtga gttcgacccg catcgcttgc cgcaggttct tcacctcgcg
     4681 ctcgtcgacc cacaggtccg agtcgcgcgt cacgcgaaat tgggagaagc ccaccatctt
     4741 gcggccgggg aacacatcgc ccaagtgtgc gcgtatcacc gaggtgagca tcgcgaacgc
     4801 ctgctttccc ggcgcgaggc gcgccggcag cggaatcacg cgcggcaaca cgcgcggaac
     4861 attcagtatc gcggtcgcgg ccgggcggcc gtaggcgtcc ttgccttcga gcgcgacgat
     4921 gaaattcaac gctttgttca tcacctgcgg gaatggatgc gacggatcga ggccgattgg
     4981 cgccagcagc ggcctgacct cgctctcgaa atactcacgc acccagcccc gctgcgccgg
     5041 cgtgcgttcg gcgtggctga cgacgacgat tccgcgccgc gcgagccgcg gcatcagtac
     5101 gtggttgaac agcgcgtact gccgctcgac cagccgctgc gcgcgcgcgg aaatctgcag
     5161 gtaatgctcc cattgcgacg cctgcgggcc ccaacctccg cccacgccga cgtcgtcgcg
     5221 catctcgtcg atcagcccgg acacccggat ctcgaagaat tcgtcgagat tcgacgacac
     5281 aatgcagacg aaccgcagcc gctccgcgag cggaacgtcg ccgcgctcgg cgagcgcaag
     5341 cacgcgttcg ttgaatgcga gctgcgacag ttcccgattg aagtagcgtt ccgggggcgc
     5401 ggcgacgggc ctgatcttgc tgtccttgcg gccctggccg agccgatgcc tgatcctgtg
     5461 cttccagcgc acgatgcacg atgccgatga cgggaagatg acgaaccggg ctaatacaat
     5521 ccggccgcgc tgttcgattc taggcgccag ctcttcaccg cccgcgccgt gctgtcgccc
     5581 gcatgaacga aaccctaccc gaatcgccgc cgctgatcgt ggtcgagcgc gtccggaagc
     5641 gctacggcga ggccgccccc acgatcgacg acgtgagctt caccgtggcg cgcggcgagt
     5701 ttctgcttct gaccggcccg agcggagccg gcaaatcgac actgctgaga ctcctcgcgg
     5761 cgctggaatc tccctcgagc gggcgcatcg tggtcgccga tacggaactg gcgaagctga
     5821 accagcgcgc gcgtgcgctg ttgcgccgtt cactcgggat cgtgccgcag gatttgctgc
     5881 tgctgcacga tcgcacagcg ctcgaaaacg tgatgctgcc ggcgctgatt gctggtctcg
     5941 cacgctccga agccgagagc cgcgcgctgg ccgcgcttgc gcgcgtggac gttgtcgccg
     6001 cagatcaata ccccccggcg ctttcgggtg gagcgcaaca gcgggtcgcg ctggcgcgcg
     6061 cgatcgtcaa caggccaacg gcgatcctcg ccgacgagcc gactgcacac ctggatgacg
     6121 cggcagcgtc tggcatcgtg cggctgctcg acgagttttc ggcggcgggc gtcgcggtcg
     6181 tactcgcatc ccacggcgaa agcgtggcgc tgcccgcgcg cacgcgcgta ttgcgactca
     6241 ccaacggcag gaccgcagca tgagcggcgc ggcccgcctt tcgcatgctc tgcgcgaggc
     6301 aggccggctg ctgcgccggc gcagcgcaac gttttcgctt gcggtgctgc tcgctgcgtg
     6361 cgcgctggta ttgccgctcc tcgccatcac ggtgggctac gggctagcgc cattggcgaa
     6421 ccgagtgccg atcgcgccgg aaatcagcgt gttcgcctcg ctttcggcaa cgggccagga
     6481 catcgcgacg ctcaagtcaa agctcgaggc gctgcccaat gtcgagcgcg ttcagtggat
     6541 tacacgcgac cagtcactgg ctgagcttgc gcgccgtagc ggaggtctag cgccgctcgc
     6601 cgacgtcaaa cccaacccac tgccggacac gctggtagtg tcgctgaacc gccgcgtgca
     6661 gcccgacgac ctcgatcgcg ccgcgaacga aatccgcaag cttgcacgtg tcgacgcggt
     6721 gtacgcagat agcagttggt tccgcaaggc ggtcggcctg acgcaggtcg tcgtgaaggt
     6781 cgccgctttc gtcgggatcg cgacggtcgg cctgctcgcg ctggtagtcg tcggagcggt
     6841 gcgactgatt gcgataagcg atcgggacga actgcggctg ctgcgcctga ttggcgccga
     6901 ggaccgacag atcgcccggc cgtatgccta cgccggcggg atcgcgctgc tgctcgcgag
     6961 catgctggcg acggcgaccg tggccgccct gctgcagtgg ctgatgccgg acctcgattg
     7021 gctggcgcaa gtgctcgcgg tgcaaatcgg tctaggcccg ttgccatggc cgacgttggc
     7081 ggggttggcc gcggtcgcgt ttctgattgg aatggcgggc ggggcgctcg gcctgcggag
     7141 cgcgctgcgt cgcatcgtcc gtaggtaacg aaacgctgcg gtacctatag tcagtgtatg
     7201 ctcactgtct cataattcaa tacaaaaagg acgttggcac tgattgaacc attcggactg
     7261 ggcagactct attcgctagc actcccggat tgagagtgct agcattttat tgatgagagg
     7321 cctagacgga atggcaactt ccttgaatgc attggtcccg gtcgggaaca gcgcgctggc
     7381 gctgcatccc ggcagcctcg gaaatctcga cgcatacatc agcgccgtca atcgcgtccc
     7441 gctgctgacg ccggacgagg agttgcggct ggcgcgagcg tttcgagcag aaggcgacct
     7501 cgaggctgcg caacggctcg tgctgtcgca tctgcgcctc gtggtagccg tggcccgcaa
     7561 ttacctgggc tatgggctgc cgcatgcgga cctgatccag gaaggcaaca tcgggctgat
     7621 gaaagcggtc aagcgcttcg atccagagcg cggggtgcgg ctcgtgtcgt tcgcgctgca
     7681 ctggatcaag gcggagatcc atgaattcat cctgcgcaac tggcggctgg ttaaggttgc
     7741 cacgaccaag gcgcagcgca aactgttctt caacctgcgc agcatgaagc aggggatccg
     7801 gacgatgtcg cccgatcagg tcgccgaggt cgcatcgacg ctgaacgtga agccggagga
     7861 agtcgtcgag atggaaaccc ggctgtccgg cggagacgtg gcgctcgaaa gccgcgccga
     7921 ggatggcgaa gacgaatacg cgccgatcgc ctatctggcg gattcgggct cggagccggt
     7981 ccaagtgctg gagcggcgcg agtatgaccg gttgcagacg gacgggatcc gcgctgcgct
     8041 cgaagggctc gacgcacgca gccgccggat catcgaggca cgctggctga acgaagacgc
     8101 gcagggcagc gtcggctcgg ccaccttgca cgatcttgcc gacgaatttg gggtttctgc
     8161 ggaacgaatt cgccaaatcg aggccaaagc gctacagaag atgcgcggcg cgctcgccga
     8221 gtacgcctga gcgccgtggt cttccgacgg ttggttgcct cgacagatcc gcctctcctc
     8281 ctccccctct cctccgaaga ggcggattct ggaccccgct acggcggggt ctttttttgt
     8341 ctgcggcggc taaccttcgt ctcatgaggc cgtcggggtt cgttcggctc gaggtgccgc
     8401 cggccgtctg cgagggcacg gacggcaagc gctcgtccgc gcgttgcgtg tgggtggcca
     8461 gcatcctgcc ggacgactcg ctcactgaat cggcgcttct attcgcggac cgttgccctc
     8521 ggacgccggg acgcgtgcac ggtttggggg acctccttga ccccggagtg catgctgcac
     8581 ttctggcaca actgccgccg ggcaagggct tgcgcctgcg tgagcgcttc gagtggtatg
     8641 cgtgccgcgg ggccggattc cacacggatg cgcactatgg cggcgtcctg ttcggcgcgt
     8701 ggtgcgtggc tggcccgaag cgcgaaatcg tgttctcgcg ctcgggactt cgcgtcggcg
     8761 cggcggtcgg agaacttgtc gtgttcgatc cgttcgagcc ccatgccgtg ctcgatccgg
     8821 gtcaggcgcg gtacgcgcgg gagcatttcg agcgggcgcc ggtgagcctc ttccttggct
     8881 tcgagatcga cctcgacgcc gccgcgcgcg tcgcattcgg catcggagcg ccgcccgccg
     8941 gcggcgcgat cctgtcgagc gcggtcgcgg tcaatgcgga gaccggcgcg ttgcccggtt
     9001 cgcgctagag gagtttcttg aggtcggcga cgatcgcatc gatatcgccg ccgtgcttca
     9061 ggaacaatcg cagacggcct tctttgtcgt aaacgaacgt tccagcagtg tggtcgatcg
     9121 tgtaggacgt gggcgtcttg cccgcgacct tctgataaaa gaccttgtat tccttcgccg
     9181 tacgcgcgat ttcctcgtcg gtgccataca ggccgacaaa gctcggatcg aacccgggaa
     9241 cgtactgcgc cagcacctcg cgcgagtcgc gggcgggatc gaccgtaatg aacaacacct
     9301 ggagccggtc gccgtccgtg ccgagcttct tcttgacctg cgccatgtcc gccagcgtgg
     9361 tcgggcagat gtcaggacac tgcgtaaacc caaagaagat cgccacgacc ttgccgcgat
     9421 agtcggccag cgtgcgcctg gcgccggtat ggtcggtcag ggtgaagtcg cgagcgaaat
     9481 ccgcgccggt gacgtcgacg ttgttgaacg tcggcggcga gcacgcggca aggaccgcga
     9541 tgccgaggat ccagagaaga cggcgcacgg cgtccacgcc cggcacagaa ccgaagatca
     9601 gcccaggtac ttgtcgatca gcagcgctgc gaacagcgcc gccaggtaga cgatcgagta
     9661 gttgaacgtg gatcgggcaa gcccgtcgct gtaaccgcgc agcagccgtc cggcgtagac
     9721 aaggaacatc gctccgagtg ccagcgcggc ccccaggtac agcgcgccgg ccatctggat
     9781 cacgaacggc agcatcgtca ccgcgaccag cacgatcgtg tacagcagga tgtgcagccg
     9841 cgtcaattcg gaaccgtgcg tgaccggcag catcgggagg ccagacttgc gatagtcgtc
     9901 cagccggtac agcgccagcg cccagaaatg gggtggcgtc cacacgaaga tgatcagtgc
     9961 cagcacccag gcctcggcgg gcgcggcgtt ggtgattgcc gcccagccca ggaccggcgg
    10021 catcgcacca ctcatgccgc cgatcacgat gttctgcggc gtgtacggct tcagcagcac
    10081 cgtatagatc acggcgtagc caacgaaggt cgcgaacgtg agccacatcg tcagcgcgtt
    10141 gacctgcgtg taaagcaccc acattccggc gccgccgata aggcccgaaa agaacatcgc
    10201 ctgcgcaggt gtgatctctc cgcgcgccac cggtcgcatc cgggtgcgct gcatccgagc
    10261 gtcgatatgc cgctcgatga tggagttgac cgcgaacgca gcgcccgcca gcagccagat
    10321 gcctgcggtc gccttcagta ccagcccgac cgccggcaac tcgggcgtcg acaaaaacat
    10381 cccaatcacc gcgcaaaaca gcgcgagttg ggtcacccgt ggcttggtca gcagccagta
    10441 ttgccgggcc aggtgcgcga ccggcgcaat gtattcgata cttgtgtggg tttgcatcgg
    10501 tatcgggaca aattcgccgc aacttcgagg gcgaaacggc gaattctacc cgccgtcgag
    10561 gaccgccgcc cgtcccgccg acccgatccg atagagcacg acgaccatga tggcgatcag
    10621 aagtgcggca ctgccgttat gcacaacggc gagcaacagc ggccattgca gcaccacgtt
    10681 ggaaaggccc gtcagaaatt gcgccgccag cagcatggca agccagcgcg cgggtccacg
    10741 cagaccctcg agtctccacg cgcgccaagc cagcgtaccg agcacgacga atgccgccag
    10801 cgaaaatgcg cggtgcgccc aatggatcgc caccagcgcc tggaacggaa tcggcgcgcc
    10861 gtcgccggtc atgccgaggt cgcgccacag cgtgaacccg tgtcggaaat cggctggcgg
    10921 catcaattcg ccttggcaga gcggaaagtc aggacatgca agcaccgcgt aattggtgct
    10981 gacccagccg ccgagcgcaa tctgcagggc gagcacgatc agccccgcgg cggcgtgaac
    11041 gcctatatcc gttgcacgcg gcgcaacacg acgcgacggg ctttcgcgca gcgcgaacca
    11101 ggtaagcagc gccaacaatg cgacgccgcc gagcaggtgc gtcgtgacga tcacgggctg
    11161 cagcttcatt gtcaccgtcc atgccccaaa cgcaccctgc aggcacacgg tgagaaaggt
    11221 cgcggtgatc agcaagggcg ccggatgcga acgatcgcgt cgccaacgca tccagcacgc
    11281 ggccatcagc acgatgatca ggaagccgac cgccatcgcg aaataccggt gcagcatctc
    11341 gatccaggcc ttggtcatcg tgacggggcc ggtcggctgc gccgtctgcg cagcgcggat
    11401 tggttcgccc gcagacagcg ggttggaatg cccatagcag cccggccagt ctgggcagcc
    11461 gagccccgag tcagtcagcc gcgtgaagct gccgaacatc accaggtcga gtgtcagaaa
    11521 cgcggtgacc catgccagct tgcgcagctt ggccggatcg cgggagcgcc agacgtaagc
    11581 gagaggaagc gcggcgaccg cgatcgcctt gagcgcgagc cacagcagcg cgtcagcctg
    11641 gatcatcagc cgatccccga ggccttcagc aggcgggcca gatcgcgctt cattcggttc
    11701 ggatccgcgt ccttcggcca acgcagcatc aagttgccga gcggatctat gacccagatg
    11761 tgatcggtca accggcctcc ggcaggcaac gggagatagg actcaagctg cgccggatcg
    11821 gcgcgcagga agcgggtacc gtcgaactcg cgcatcacga tggtcggcag cggcgcagcg
    11881 tcgtcgacca gccagatccg ctcgacgcgg tcggcgtcct tcccggtggt cagacgcacc
    11941 tgccgcatct gccatagctt gcgctggcag gcgtcgcggc agtcgccgct gtccacggtg
    12001 accagcagcc agcgaccgcg cagcgcagat gttgccacgt cgcgcccatc gagttcgcgc
    12061 agcgcgagcg caggcatcgg gcgctgcggc tcgac
//
LOCUS       DBNHBY010000215         4430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_282859, whole genome shotgun sequence.
ACCESSION   DBNHBY010000215 DBNHBY010000000
VERSION     DBNHBY010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_282859"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="ytpR"
                     /locus_tag="AC16HB_05300"
     CDS             complement(<1..823)
                     /gene="ytpR"
                     /locus_tag="AC16HB_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIR5606241.1"
                     /translation="MQFSEQWLRMYVDPPIGSDELAHRLTMAGLEVEANEPVAPAFSN
                     VVVARVLSVAKHPAADKLTVCQVDAGAGTPLAIVCGAPNVAAGIRVPCALPGAQLPGD
                     FRIKATTMRGVESQGMLCSARELGLSEDHSGLMILTADAPIGRDVREYLALEERKFTI
                     KLTPNRGDCLSVVGVAREVAAITGAALALPAFAAVKPTIDERLRVRIDAPDLCGRFSG
                     RVVRGLNAGAPTPEWMKQRLERSGQRPISALVDISNYVMLELGRPSHIFDLDKVHG"
     gene            complement(839..1855)
                     /gene="pheS"
                     /locus_tag="AC16HB_05305"
     CDS             complement(839..1855)
                     /gene="pheS"
                     /locus_tag="AC16HB_05305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR5606242.1"
                     /translation="MERLEQLIDEDRAAFAAVDLPAALENEKARFLGKSGSVTELLKG
                     LGKLPPEEKRAQGARINAAKEAIERLLAERRAQLAEAELAVRLAQESIDVTLPGRKRG
                     AGGIHPVVRTWMRIEEIFGSIGFDVADGPEIETDWFNFTALNNPPNHPARSMQDTFYV
                     DMNGSDGLPLNLRTHTSPMQVRYARMHQPPLKVIAPGRTYRVDSDATHSPMFHQVEGL
                     WIDEDVSFADLKGVYTDFLRRFFESDDLQVRFRPSFFPFTEPSAEIDMMFTSGPNKGK
                     WLEISGAGQVHPTVVRNYGLDPERWIGFAFGSGLERLTMLRYGVDDLRLFFEGDLRFL
                     SQFN"
     gene            complement(1940..2299)
                     /gene="rplT"
                     /locus_tag="AC16HB_05310"
     CDS             complement(1940..2299)
                     /gene="rplT"
                     /locus_tag="AC16HB_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR5606243.1"
                     /translation="MPRVKRGVTARARHKKVLSAAKGYTQRRKSVFRVAKEAVMKAGQ
                     YAYRDRRNRKRVFRSLWIARINAAVREQGLSYSSFIAGLKKAAIDMDRKVLADMAVLD
                     KPAFAAIVDKVKSSLAA"
     gene            complement(2309..2506)
                     /gene="rpmI"
                     /locus_tag="AC16HB_05315"
     CDS             complement(2309..2506)
                     /gene="rpmI"
                     /locus_tag="AC16HB_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR5606244.1"
                     /translation="MPKMKTKKSAAKRFVVRNSGSIKRGQAFKRHILTKKTTKSKRHL
                     RGPVELHKSDVESVRRMLPYA"
     gene            complement(2619..3140)
                     /gene="infC"
                     /locus_tag="AC16HB_05320"
     CDS             complement(2619..3140)
                     /gene="infC"
                     /locus_tag="AC16HB_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR5606245.1"
                     /translation="MAQDRSHRLNREITAPEVRLTGVNNEVLGIVSLAEALRLAEEAD
                     VDLVEIAPNASPPVCRLMDYGKFKYQEQKKAHEAKLKQKVIEVKEIKFRPATDDHDYQ
                     TKVRKLHEFLEEGDKAKVTMRFRGREMAHQELGLKLLERVKSDLQEVSQVEAMPKLEG
                     RQMVMVLAPKKKK"
     gene            complement(3169..>4430)
                     /gene="thrS"
                     /locus_tag="AC16HB_05325"
     CDS             complement(3169..>4430)
                     /gene="thrS"
                     /locus_tag="AC16HB_05325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5606246.1"
                     /translation="AKKEDQDAYLKMLEEAERRDHRKLGRELDLYHFQDEAPGLIFWH
                     PRGWAIWQSVEQYMRQVYRDNGYQEVRAPQILDRSLWEKSGHWEKYRENMFTTESENR
                     YYALKPMNCPGHIQIYNANLHSYRELPLRYGEFGACHRNEASGALHGLMRVRGFTQDD
                     GHIFCTEEQILDECVAYTALLQKVYRDFGFEHIRYKVSTRPDKRIGSDESWDKAEQAL
                     MESLRRSGVEFTISPGDGAFYGPKIEYTLKDAIGREWQCGTMQVDFSMPGRLGAEYVD
                     EDNTRKVPVMLHRAIVGSLERFIGVLIENYAGALPPWLAPAQVAVANISEHQVAYAGD
                     VAQTLKKQGFRVEADLRNEKITYKIRALALQKIPYILVVGDKEMQAGQVAVRARGGVD
                     LGVMSREAFAARLAEDVAQRRNVGSAA"
BASE COUNT          717 a         1518 c         1329 g          866 t
ORIGIN      
        1 tcccgtgcac cttgtcgagg tcgaaaatgt gcgaaggccg cccgagctcg agcatcacgt
       61 agttggagat gtcgaccaac gccgaaatcg gacgctgccc gctgcgctcc aggcgctgct
      121 tcatccactc cggcgtcggc gcgccggcat tcaggccgcg cacgacgcgg ccggaaaagc
      181 gcccgcacag gtcgggcgcg tcgatcctca cgcggagtcg ctcgtcgatc gtcggcttga
      241 ccgctgcgaa cgccggcagc gccagcgccg caccagtgat cgccgcgacc tcgcgcgcca
      301 cgccgaccac gctcagacaa tcaccccggt tgggcgtgag cttgatggtg aacttgcgtt
      361 cctcgagtgc gaggtattcg cgcacgtcgc ggccgattgg cgcgtcggcg gtcaggatca
      421 tcaatccgct gtggtcctcc gacagcccga gttcgcgcgc cgagcaaagc atcccctgcg
      481 attccacgcc gcgcatggtc gtcgccttga tgcggaagtc gcccggcagt tgcgcccccg
      541 gcaatgcaca gggcacgcgg attcctgccg cgacgttcgg cgcgccgcac acgatcgcga
      601 gcggggtgcc ggcgccggca tcgacctggc acacggtcag cttgtctgcg gccggatgct
      661 tcgcgaccga cagcacgcga gcgacgacga cgttgctgaa cgcgggcgcg accggctcgt
      721 tcgcctcgac ctcgagcccc gccatcgtca gccgatgcgc gagttcgtcg ctgccgatcg
      781 gcgggtcgac gtacatgcgc agccactgtt ccgagaactg catcgctgat ctcccgggtc
      841 agttgaactg cgacaggaag cgcaggtcgc cctcgaagaa caggcgcagg tcgtcgacgc
      901 catagcgcag catcgtcagc cgttccaggc cggaaccgaa cgcgaatccg atccagcgct
      961 cgggatcgag cccgtagttg cgcacgaccg tcggatgcac ctggccagca ccggagatct
     1021 ccagccactt gcccttgttc ggtccgctgg tgaacatcat gtcgatctct gcggacggct
     1081 cggtgaatgg gaagaacgac ggccggaagc gcacctgcag gtcgtcgctc tcgaagaagc
     1141 gccgcaggaa gtccgtgtac acgcccttca ggtcggcaaa gctcacgtcc tcgtcgatcc
     1201 acagaccctc gacttgatgg aacatcggcg agtgcgttgc atccgaatcg acgcgatacg
     1261 tacggcccgg cgcgatcacc ttgagcggcg gctggtgcat gcgcgcatag cgcacctgca
     1321 tcggtgacgt gtgggtacgc agattcagcg gcagcccgtc actgccattc atgtcgacgt
     1381 agaaggtgtc ctgcatcgag cgcgccggat ggttgggcgg gttgttcagg gccgtgaagt
     1441 tgaaccagtc ggtctcgatt tccggcccgt cggcgacgtc gaagccgatc gagccgaaga
     1501 tctcctcgat ccgcatccac gtgcggacca caggatggat gccgcctgcg ccgcgcttgc
     1561 gccccggcag ggtgacgtcg atcgactcct gcgccagccg cacagcaagt tccgcttcgg
     1621 ccagttgcgc gcgccgctcg gccagcagcc gctcgatcgc ttccttggcg gcgttgatgc
     1681 gcgcgccctg cgcccgcttc tcctccggcg gcagtttgcc gagccccttg agcagttcgg
     1741 tcacgctgcc gctcttgccc aggaagcgcg ccttctcgtt ctccagcgcg gccggcaggt
     1801 ccaccgcagc gaacgccgcg cggtcctcgt caatcagttg ttcgagtcgt tccatcagtg
     1861 atctcaaaac gaaaaacggg gcccgccgcc aaggccaggc cccgatcttt cggctgcgcg
     1921 cgcacgaggc gcgcttgcat caggcagcga ggctggactt gaccttgtcg acgattgccg
     1981 caaatgccgg cttgtcgagt acggccatgt ccgcgaggac cttgcggtcc atgtcgatgg
     2041 cggccttctt caggcccgcg atgaacgacg aatacgacaa gccttgctcg cgcaccgctg
     2101 cgttgatgcg tgcgatccac agcgaacgga acacgcgctt gcggttgcgg cgatcgcggt
     2161 aggcgtactg ccccgccttc atcaccgctt ccttggcgac gcggaagacg ctcttgcgcc
     2221 gctgcgtata ccctttggcc gcggacagga ccttcttgtg ccgcgcgcgc gcggtcacgc
     2281 cacgtttgac tcgaggcatg gtgtctcctt acgcgtaggg cagcatccga cgcacggatt
     2341 cgacgtcgga cttgtgcaat tcgaccggac cgcgcaggtg ccgcttggac ttggtggtct
     2401 tcttggtgag gatgtggcgc ttgaacgcct gtccgcgctt gatcgatccg ctgttgcgga
     2461 cgacgaagcg cttcgcagcg ctcttcttgg tcttcatctt gggcatgtgg gccctccttt
     2521 cttggatgac ggcaggcgct cccgcgggag actttctggc ccgccttcac ttgtaatcgc
     2581 ggcgcgccgc cgaccaagcg gcgcaccacg gcgaaacact acttcttctt cttcggcgcc
     2641 agcaccatca ccatctggcg tccttcgagc ttcggcatcg cctcgacctg gctcacctcc
     2701 tgcaggtcgg acttcacgcg ctcgagcagt ttcaggccca gttcctggtg cgccatctcg
     2761 cgcccgcgga accgcatcgt gactttcgcc ttgtcgccct cctcgaggaa ctcatgcagc
     2821 ttgcgcacct tggtctggta atcgtgatcg tcggtcgcgg gacggaactt gatttccttg
     2881 acctcgatga ccttctgctt gagcttcgcc tcgtgcgcct tcttctgttc ctgatacttg
     2941 aacttgccat agtccatcag gcgacagacc ggtggcgaag cattcggcgc gatctcgacg
     3001 aggtccacat cggcttcctc ggccaaccgc agcgcctcag ccaggctgac gatacccagc
     3061 acttcgttgt tgacacccgt cagccggact tccggggccg tgatctcgcg attgagtcga
     3121 tgcgagcgat cctgagcgat ggtctttgac tccgaagtaa agaagaaact aagccgcgct
     3181 tcccacgttg cgccgctgcg cgacgtcttc tgccagtcgc gcggcaaaag cctcccggga
     3241 catgaccccg agatcgacgc cgccgcgggc cctcacggcc acctgtcccg cctgcatttc
     3301 cttatccccc acaaccagga tgtacggaat cttctgcaaa gcgagggcgc ggattttata
     3361 agtgatcttc tcgttgcgca aatcggcttc taccctaaag ccttgttttt tcaatgtttg
     3421 cgcaacatcg cccgcatacg ccacctggtg ctcggagata tttgccaccg ccacctgcgc
     3481 cggtgccagc cacggtggca gcgcgcccgc gtaattctcg atcagcacgc cgatgaagcg
     3541 ttccaacgag cctacgatcg cccggtgcag catcaccggc accttgcgcg tgttgtcctc
     3601 gtccacgtac tctgcgccga gccgccccgg catcgagaag tcgacctgca tcgtgccgca
     3661 ctgccattcg cggccgatcg cgtccttcag cgtgtattcg atcttcggcc catagaacgc
     3721 accgtcgccc ggcgagatcg tgaactcgac gccggagcgg cgcagcgact ccatcagcgc
     3781 ctgctcggcc ttgtcccagc tttcgtcaga gccgattcgc ttgtccggcc gcgtcgacac
     3841 cttgtaccgg atgtgctcga agccgaaatc cctgtaaacc ttctgcagca gcgccgtgta
     3901 cgcgacgcac tcgtcgagga tctgctcctc ggtgcagaag atgtgaccgt cgtcctgtgt
     3961 gaaaccacgc acgcgcatca ggccgtgcag tgcacccgac gcttcgttgc ggtggcaggc
     4021 gccaaactcg ccgtatcgca gcggcagttc gcgatagctg tgcagattgg cgttgtagat
     4081 ctggatatgg ccggggcagt tcatcggctt cagcgcgtag taccggttct ccgactcggt
     4141 cgtgaacatg ttctcgcggt acttctccca gtgccctgat ttctcccaca gcgagcggtc
     4201 gaggatctgt ggcgcgcgca cctcctggta gccgttgtcg cgatacacct gcctcatgta
     4261 ctgctcgacc gactgccaga tcgcccagcc gcgcggatgc cagaaaatca gccccggcgc
     4321 ctcgtcctgg aagtggtaca ggtcgagctc gcgtcccagc ttgcggtggt cgcgccgctc
     4381 cgcctcctcc agcatcttca ggtacgcatc ctggtcctcc ttcttggccc
//
LOCUS       DBNHBY010000216         9589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_283102, whole genome shotgun sequence.
ACCESSION   DBNHBY010000216 DBNHBY010000000
VERSION     DBNHBY010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_283102"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="AC16HB_05330"
     CDS             <1..735
                     /locus_tag="AC16HB_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR5606247.1"
                     /translation="ALPPDGVAVFPADDACAGIWRNLAGTRRVLDFALHAYAAITAQH
                     EQTDNGSRIDLATPVGEIEVTLKIPGVHNVHNALAATAAALAVDVAPATIAAALATFT
                     PASGRGVRHLLASGATLIDDTYNANPDSVRAAIDVLAGCSAPRVLVLGDMGEVGAQGP
                     QFHREVGAYARERGIERLLCLGDASRETAAAFGSDAQHFDAVELLIDACRAAAAAEAT
                     LLVKGSRFMRMERVVQALSNTTAGAH"
     gene            740..1894
                     /gene="mraY"
                     /locus_tag="AC16HB_05335"
     CDS             740..1894
                     /gene="mraY"
                     /locus_tag="AC16HB_05335"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5606248.1"
                     /translation="MLLELFQWLSQDIRAFRVFDYLTLRAVLAAATALVIGLFAGPKV
                     IRRLTELKIGQAVRTEGPQSHLSKSGTPTMGGALILIAIGITTLLWADLTNRFVWVVL
                     LVTLGFGWIGWIDDYRKVVHRNPKGMSAKEKYFWQSLIGLTAAFYLAFAIAAPSTDKI
                     WLLMGQWIAHGFDIDLPPRSDLIVPVFKNYAYPMGVFGFIVLSYLVIVGTSNAVNLTD
                     GLDGLAILPASMVGAALGVFAYVVGRADFSKYLLFPYIPGAGELVIICAAIAGAGLAF
                     LWFNAYPAQVFMGDVGALALGAALGTIAVITRQEIVLFIMGGVFVAETVSVMLQVSYF
                     KWTKGRRIFRMAPLHHHFELSGWKENQVVVRFWIITIMLVLVGLSTLKIR"
     gene            1903..3381
                     /gene="murD"
                     /locus_tag="AC16HB_05340"
     CDS             1903..3381
                     /gene="murD"
                     /locus_tag="AC16HB_05340"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5606249.1"
                     /translation="MHVLVLGLGESGLAMARWLEREGARLRVADTRATPPMLDALRAQ
                     VPLAEFSGGAFDGALLESIDCIAISPGLSLTASPAARLLKEAKARGIEVVGEVELFAR
                     ALARLRAERGYAPRVLGITGTNGKTTTSRLTGLLVERAGQSVAVAGNISPTALDALRE
                     HLDADELPSAWVLELSSFQLAATFSLACDAAAVLNVTQDHLDWHGSIDAYAKAKARIF
                     GKDTVQVLNRDDPIVLAMARKSAHVCTFGTDAPVHADQYGLLRDGALTWLAYAEQTGG
                     QRRRRSAADAAAPFAEAPTFVHRLMPTEALRLRGRHNALNALAALALARAIACPLAPM
                     LHALREYRGEPHRVELVANFRGVEYYDDSKGTNVGATVAAIEGLGAEGKRIAIILGGD
                     GKGQDFAPLAAPLARHARAVALIGRDAERIAAALDAKYPIERCAALSDAVRVCARHAR
                     SGDAVLLSPACASLDMFRNYAHRSEVFVDAVRELGMEAGQPC"
     gene            3375..4640
                     /gene="ftsW"
                     /locus_tag="AC16HB_05345"
     CDS             3375..4640
                     /gene="ftsW"
                     /locus_tag="AC16HB_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR5606250.1"
                     /translation="MLGRLWQRLRGGARRRRETDAPSNSVLAFAGLGAVPVSARTPRS
                     AFERYDAPLAMVASALILIGVVMVYSASISLADSSKYTVTSTHFVARHMFALGMAFLV
                     ATVAFSIPMARWQRLAPWMFLAGLALLVIVLVPGVGKGVNGARRWIPLGMLNLQPSEV
                     MKVACVLYAANFTVRKQDFMHHFGKGFAPMAAVMALLGTLLLLQPDLGAFGVLVAISM
                     GILFLGGVNGRLFAGISATLATAFVLVIWLSPWRRERIFAYIDPCAPEQALSKGYQLC
                     TSLISFGRGELFGVGLGGSVVKLSYLPEAHTDFIFAVIGEELGLAGVLTVMVLFYWLT
                     KRAFEIGRQAVALERTFAGLVAQGVGLWIGVQSFVNMGVATGLLPTKGLTLPLVSYGG
                     SALLSTITALGLLLRVDYENRILMRGGRA"
     gene            4637..5722
                     /gene="murG"
                     /locus_tag="AC16HB_05350"
     CDS             4637..5722
                     /gene="murG"
                     /locus_tag="AC16HB_05350"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR5606251.1"
                     /translation="MRHLVVVAAGTGGHVMPGLAVAGELRARGWTVSWLGTQAGLERR
                     LVEPQGIEFDALDFSGLRGKGFKTLLLGGFQLLHALWQSRAALRVRRPALVFSTGGYV
                     AVPAGLAASLHGAPLALLNADAAPLLSTKMLRSLASAVFCGFDGEAARLAGAKGLVTG
                     NPVRDEIARVTAPDERYAQRQGPLNLLVVGGSLGAQVLNQSLPAALARLAADTRPRVV
                     HQCGAAHVDATRAAYGALNVEAQVIPFIDDIAAQYAAADVVLCRAGAITVTELCVAGV
                     ASILVPFVVLTTAHQRTNAEFLAQHGAAIHLPQADLTADRLAQSLRGLTRAQLAAMAR
                     KARALGRPDATRVVADAIEHIAQEAAR"
     gene            5838..7229
                     /gene="murC"
                     /locus_tag="AC16HB_05355"
     CDS             5838..7229
                     /gene="murC"
                     /locus_tag="AC16HB_05355"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5606252.1"
                     /translation="MKHAIKHIHFVGVGGSGMSGIAEVLLNLGYAVSGSDAASSAVTR
                     RLARLGVRVAHGHAAENIAGADAVVVSTAVGADNPEVRAARAAHIPVVPRAMMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAAAGLDPTFVIGGRLNAAGANARLGQGEFIVVE
                     ADESDASFLNLLPVIAVVTNIDNDHMETYGHDFGRLKQAFIEFLSRLPFYGTAVLCID
                     DPNVREILPFVSKPVVTYGFAPEAHFRAVEAVPDGTAMRFTVQRESAASLGVRLNLPG
                     RHNVCNALAAIAVATELGVPDAAIRDALASFTGVGRRFARHGEVEIAGGRFTLIDDYG
                     HHPVEMAATLAAARGAFPGRRIVLAFQPHRFTRTRDCFEDFVKVLSTADALLLADVYP
                     AGETPIVAADGRSLARAVRVAGKVEPVFVESIDDMPAAILAAARPDDVVITMGAGSVG
                     AVPAKLTHAGGAE"
     gene            7226..8191
                     /locus_tag="AC16HB_05360"
     CDS             7226..8191
                     /locus_tag="AC16HB_05360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR5606253.1"
                     /translation="MTMTIDPKSFGRVGVLFGGRSSEREVSIMSGTGVLDALKSRGVD
                     AHAFDPGAQSLAELEAARFDRVFIALHGRFGEDGTIQGVLETLRIPYTGSGVQASAIA
                     IDKLTTKKIWLAEELPTPAWRVVHAHTDWMQLVAELGDQLIVKPSREGSTIGITKVIG
                     HDRNELAMAFEAAVKYDDCVIVEELIRGRELTCAILGEGATARALPLIEIRAPAENYD
                     YHSKYFSDETKYLCPAPLDDVLAEELQDLCVRAYNALGARGWGRIDLMLNQDPRGDRP
                     YLLELNTSPGMTGHSLVPMAAKAIGVTYEELVLEILSSARLEVPA"
     gene            8206..8943
                     /locus_tag="AC16HB_05365"
     CDS             8206..8943
                     /locus_tag="AC16HB_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5606254.1"
                     /translation="MQWATRALLAATGVLVLSAAALWVAQRPQFGIRRIELRGADGAL
                     QHVSAATVRAALRGDGRTRLRGGYFTLRLDDARRVFETVPWVAGVSVRRGWPDRLVVT
                     FTEHRALGTWVDGRLLSDAGVLFVANPAEADVDGALPEFDGPERFAPDVARRFREFTS
                     TLAPLQLTVESVEVSERAAWRLRTDTEQTIELGRDDPAGRIAQRLAAFVASYPLVLAR
                     TQGAPPRIDLRYANGFAVALPATHRKP"
     gene            8940..>9589
                     /gene="ftsA"
                     /locus_tag="AC16HB_05370"
     CDS             8940..>9589
                     /gene="ftsA"
                     /locus_tag="AC16HB_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5606255.1"
                     /translation="MKNENKDLVVGLDIGTSKVVVAVAEVLPDGRFQVIGLGQAASEG
                     LRKGVVVNIEATVQSIRRALEEAELMAACRITNVFTGIAGSHIKSFNSSGMVAIKDRE
                     VTASDVARVIETARAVNIPTDQQVLHVLTQEFIVDGQEDIREPIGMSGVRLEVKVHIV
                     TGAVSAAQNIIKCVRRCGLEVQDLILQPLASSLSVLTPDEKELGVALVDIGGGTSD"
BASE COUNT         1424 a         3122 c         3408 g         1635 t
ORIGIN      
        1 gctttgccgc ccgatggcgt ggcggttttt cccgctgacg acgcctgcgc cggcatctgg
       61 cgcaacctcg ccggcacgcg gcgcgtgctc gactttgcgc tgcacgccta tgcggcgatc
      121 acggcgcagc acgagcaaac ggacaacggc agccgcatcg acctggcgac gccggtcggc
      181 gagatcgagg tcacgctgaa gattccgggc gtgcacaacg tgcacaacgc actggccgca
      241 accgctgcag cgctcgccgt cgatgtcgcg ccagcgacga ttgccgccgc actggcgacg
      301 ttcacgccgg ccagcggacg cggcgtgcgc cacctgcttg cttccggcgc gacactgatc
      361 gacgacacct acaacgccaa tcccgactcg gtgcgcgcgg cgatcgacgt actggccggc
      421 tgcagcgcgc cgcgcgtgct ggtgctcggc gacatgggtg aggtcggcgc gcagggtccg
      481 cagttccatc gcgaggtggg cgcctacgcg cgcgagcgcg gcatcgagcg gctgctgtgc
      541 ctcggcgacg catcgcgcga gaccgccgcg gccttcgggt ccgacgcgca acacttcgat
      601 gcggtcgagc tgctgatcga cgcgtgccgc gccgccgccg ctgcggaggc aacgctgctg
      661 gtcaaggggt cacgtttcat gcgcatggag cgcgtggtgc aggcgctttc gaataccacc
      721 gcgggagcgc actgacacga tgctgctcga actcttccaa tggttgtcgc aggacattcg
      781 ggcgttccgc gtgttcgact acctgacgct gcgcgcggtg ctcgccgccg ccaccgcgct
      841 ggtgatcggt ctgtttgccg gcccgaaggt gatccgcagg ttgacggagc tgaagatcgg
      901 acaagccgtg cggaccgagg gtccgcaatc gcatctgagc aagtccggca cgccgacgat
      961 gggcggcgcg ctgatactga tcgcgatcgg catcacaacg ctgctatggg cggatctcac
     1021 caatcgcttc gtctgggtgg tgctgctggt cacgctcggc ttcggctgga tcgggtggat
     1081 cgacgactat cgcaaggtcg tgcatcgcaa cccgaagggc atgagcgcga aagagaagta
     1141 cttctggcag tccctgattg gcctgacggc cgcgttctat ctcgcgttcg cgatcgctgc
     1201 gccgtcgacc gacaagatct ggttgctgat gggccagtgg atcgcccacg gtttcgacat
     1261 cgacctgccg cctcgctccg acctgatcgt gccggtcttc aagaactacg cctatccaat
     1321 gggcgtgttc ggcttcatcg tgctgtcgta tctggtgatc gtcggcacca gtaacgcggt
     1381 gaatctgacc gacggtctcg acggcctggc catcctgccg gcgtcgatgg tcggcgccgc
     1441 cttgggcgtc tttgcgtacg tggtcggacg cgcggacttc tcgaagtacc tgctgttccc
     1501 ttacatcccc ggtgcgggcg agctggtgat catctgcgcg gccatcgcgg gggcggggct
     1561 cgcgttcctc tggttcaacg cgtatccagc gcaggtcttc atgggcgacg tcggtgcgct
     1621 agcgctcggc gccgccctcg gcaccatcgc ggtgatcacc cgacaggaga tcgtgctctt
     1681 catcatgggc ggcgtgttcg tcgccgagac cgtgtcggtg atgctgcagg tcagctattt
     1741 caaatggacc aaggggcgcc gcatcttccg catggcgccg ttgcaccatc acttcgaact
     1801 gtcgggctgg aaagagaatc aggtggtcgt gcggttctgg atcatcacga tcatgttggt
     1861 gctggtcggc ctgtcgacgc tgaagatccg gtaggcgaac gcatgcacgt actggtcctc
     1921 ggtctgggcg agagcggtct ggcgatggcg cgctggctgg agcgcgaagg ggcgcggctg
     1981 cgcgtggccg acacgcgcgc gacgccgccg atgctcgacg cgctgcgggc gcaggtgccc
     2041 ttggccgagt tcagcggtgg cgcgttcgac ggcgccctgc tcgagagcat cgactgcatc
     2101 gcgatcagcc cgggactatc gctgaccgct tcgcccgctg cacgcttgct gaaggaggcg
     2161 aaggcgcgcg gcatcgaggt cgtcggcgag gtcgaactct tcgcgcgtgc gctcgcccgc
     2221 ttgcgcgcgg aacgcggcta tgcgccgcgg gtgctgggca tcacaggcac caatggcaag
     2281 accaccacgt cccgcctgac cgggctgctg gtcgaacgtg ccggacagag cgtcgcggtg
     2341 gccggcaaca tctcgccgac cgcgctcgac gcgctgcgcg aacacctcga cgccgatgaa
     2401 ctgccgagcg cctgggtgct cgagctgtcg agcttccagc ttgcggccac gttctcgctc
     2461 gcatgcgatg cggcggccgt gctgaacgtc acccaggacc atctcgactg gcacggttcg
     2521 atcgacgcct acgcaaaggc gaaggcgcgc atcttcggca aggacacggt gcaagtgctc
     2581 aatcgcgacg atccgatcgt gctcgcgatg gcgcgcaaga gcgcccacgt ctgcacgttt
     2641 ggcaccgatg cgccggtgca cgcggatcaa tatggcctgc tgcgcgacgg cgcgctgacc
     2701 tggctcgcgt atgccgagca gacgggcgga cagcggcgcc gcagatcggc cgccgatgcc
     2761 gccgcaccct ttgcggaggc gccgaccttc gtgcatcggc tgatgccgac cgaggcattg
     2821 cgcctgcgcg gccgccacaa cgcgctcaat gcgctggccg cattggcgct ggcacgcgcg
     2881 atcgcctgcc cgctggcgcc gatgctgcat gcgctgcgcg agtaccgcgg agagccgcat
     2941 cgggtcgagc tcgtcgccaa cttccggggc gtggagtact acgacgactc gaagggcacc
     3001 aacgtcggtg cgaccgtcgc cgcgatcgaa gggcttggcg cggaaggcaa gcggattgcg
     3061 atcattctcg ggggcgacgg caaggggcag gacttcgcgc cgctcgccgc gccgctggcg
     3121 cggcacgcgc gcgccgtcgc gctgatcggt cgcgacgcgg aacggattgc agccgcgctc
     3181 gacgccaagt atccgatcga acgctgtgcc gcactttccg acgcggtgcg ggtgtgcgcc
     3241 cgccacgcaa ggagcggcga tgcggtgctg ctgtcgccgg cgtgcgcgag cctcgacatg
     3301 ttccgcaact atgcgcatcg cagcgaagtg tttgtcgacg cggtgcgcga actcggcatg
     3361 gaggccggcc agccatgcta gggcggctct ggcagcggct gcgcggaggc gcgcgtcggc
     3421 ggcgcgagac ggacgcgccg tcgaattccg tgcttgcatt cgccggtctc ggtgcggtgc
     3481 ccgtcagtgc ccgcacgccg cgcagtgcgt tcgagcgcta cgacgcgccg ctcgcgatgg
     3541 tggccagcgc gctgatcctg atcggcgtgg taatggtgta ctcggcgtcg atctcgctcg
     3601 cggattcatc gaagtacacg gtgacgtcga cgcacttcgt ggcgcggcac atgtttgccc
     3661 tcggcatggc cttcctcgtc gccacggtgg cgttctcgat tccgatggcc aggtggcagc
     3721 ggctggcgcc gtggatgttc ctcgccggct tggcgctgct ggtgatcgtg ctcgtgcccg
     3781 gtgtgggcaa gggtgtcaac ggcgcgcggc gctggattcc gctcggcatg ctgaacctgc
     3841 agccgtccga ggtgatgaag gtcgcctgtg tgctgtatgc ggcgaacttc accgtgcgca
     3901 agcaggactt catgcatcac ttcggcaagg gcttcgcgcc gatggccgca gtgatggcgc
     3961 tgctcggcac tctgctgctg ctgcagccgg acctcggcgc gttcggtgtg ctggttgcaa
     4021 tcagcatggg catcctgttc ctcggtggtg tgaacgggcg actgttcgcc ggcatctctg
     4081 ccacgctcgc gaccgcgttc gtgctggtga tctggctcag cccgtggcgg cgcgagcgga
     4141 tcttcgccta catcgatccg tgcgcgcccg agcaggcgct gtcgaagggt taccagttgt
     4201 gcacgtcgct gatctcgttc ggccgcggcg agctgttcgg cgtcgggttg ggcggcagcg
     4261 tggtcaagtt gagctacctg cccgaggcgc acaccgactt catctttgcc gtgatcggcg
     4321 aggagctcgg gctcgccggt gtgctcacgg tgatggtgct gttctactgg ctgaccaagc
     4381 gcgcgttcga gatcggccgt caggcggtcg ctctcgagcg cacgtttgcc gggctcgtcg
     4441 ctcagggcgt gggactgtgg atcggtgtgc agtcgttcgt caacatgggc gtggccacgg
     4501 ggctgctgcc cacaaagggg ttgaccttgc cactggtgag ctacggcggt tccgcgctgc
     4561 tgtcgacgat caccgcactg ggactgctgc tgcgggtcga ttacgagaat cggattctga
     4621 tgcgcggagg ccgcgcatga gacatctcgt cgtggtcgcc gcaggcaccg gtgggcacgt
     4681 gatgcccggg ctcgcagtcg ccggagagtt gcgggcgcgc ggctggaccg tttcctggct
     4741 cggcacccaa gccggcctgg agcgtcggct ggtcgagcca cagggcatcg aattcgatgc
     4801 gctcgacttc agcggcctgc gcggcaaggg tttcaagacg ctgctgctcg gcggctttca
     4861 actgctgcac gcgctgtggc agagccgcgc ggccttgcgt gtgcggcggc cggcgctggt
     4921 gttctcgacc ggcggctacg tggcggtgcc ggccggactc gcggcatcgc tgcacggtgc
     4981 gccgttggca ctgctgaacg ccgacgccgc gccgctgctg tcgacgaaga tgctgcgttc
     5041 cctcgcgtcg gcagtgttct gcggcttcga cggcgaggcg gcacggctgg ccggcgccaa
     5101 gggactcgtg accggcaatc cggtacgcga cgagatcgcg cgtgtaacgg cgccggacga
     5161 gcgctacgcg cagcggcagg ggccactgaa cctgctcgtc gtcggcggca gcctcggcgc
     5221 gcaagtgctg aaccagtcac tgccggccgc gctggcacgg cttgcggcgg acacgcgtcc
     5281 gcgcgtcgtg catcagtgcg gcgcggccca tgtcgacgcg acacgcgccg cctacggggc
     5341 gttgaacgtc gaggcgcagg tgattccgtt catcgatgac atcgccgcgc agtacgctgc
     5401 tgccgacgtt gtgctgtgcc gcgccggcgc gatcacggtc accgagctgt gcgtggctgg
     5461 cgtggcgtcg atcctggtgc cgttcgtcgt gttgacgacc gcgcaccagc gcaccaacgc
     5521 cgagttcctt gcgcagcatg gcgcggcgat ccatctcccg caggcggatt tgacggccga
     5581 tcggctggcg cagtcgctgc gcgggctgac gcgcgcgcaa ctggctgcaa tggcgcgcaa
     5641 ggcgcgggcg ctcggtcggc ccgatgcgac ccgcgtcgtg gccgacgcga tcgagcacat
     5701 cgcgcaagag gcggcgcgat gaggcacatc atcaagcaca tcctcttcct cggtacgtcg
     5761 ggtgaggtgc cccggcgtcc acatcgtgcg gctgcgccga tgctgacgaa gcgtccggct
     5821 cgatcggagg cgacggagtg aagcacgcca tcaagcacat ccacttcgtc ggcgtcggcg
     5881 gcagcgggat gtcgggcatc gccgaggtgc tgctgaacct cggctacgcg gtcagcggtt
     5941 ccgacgcggc gagcagcgct gtgacgcggc ggcttgcgcg actcggcgtg cgcgtggcgc
     6001 acggccatgc agcggaaaac atcgccggcg ccgacgcggt ggtggtctcg accgccgtcg
     6061 gtgccgacaa ccccgaagtg cgcgctgcac gcgcagcgca cattccggtg gtgccgcgcg
     6121 cgatgatgct ggcggaactg atgcggctga agcagggcat cgccatcgcc ggcacgcacg
     6181 gcaagacaac gaccaccagc ctcgtcgcca gcgtgctcgc cgcggcgggg ctcgatccca
     6241 cgttcgtgat cggcgggcgg ctgaacgcgg cgggtgccaa cgcgcgtctc gggcaaggcg
     6301 aattcatcgt ggtcgaggcc gacgaatccg acgccagctt cctgaacctg cttccggtga
     6361 tcgcggtcgt gaccaatatc gacaacgacc acatggaaac ctacggccac gatttcgggc
     6421 ggctgaagca ggcgttcatc gagttcctgt cgcggctgcc gttctatggg acagcggtgt
     6481 tgtgcatcga tgatcccaac gtgcgcgaga tcctgccgtt cgtctcgaag cccgtggtga
     6541 cgtacggctt cgcgcccgag gcgcatttcc gggcggtcga agcggttccc gacggcaccg
     6601 cgatgcgttt caccgtgcag cgcgagagcg cggcttcgct cggcgtgcgg ctgaacctgc
     6661 cggggcggca caatgtctgc aacgcgctcg ccgcgatcgc tgtggcgacc gagctaggcg
     6721 tgccggatgc agcgatccgc gatgcgctgg ccagcttcac cggcgtcggc cgccgcttcg
     6781 cgcggcacgg cgaggtcgag atcgctggcg gccgcttcac gctgatcgac gactacggcc
     6841 atcacccggt cgagatggcg gccacgctcg cggccgcacg cggcgcattt ccggggcgcc
     6901 gcatcgtgct ggcgtttcag ccgcaccgct tcacgcgtac gcgcgactgc ttcgaggact
     6961 tcgtcaaggt gctgtcgacg gcggacgcgc tgctgctggc tgatgtttat ccggctggcg
     7021 aaacgccgat cgtcgcagcc gacggccgct cgctggcgcg cgcggtccgc gtcgcgggaa
     7081 aggtcgagcc ggtattcgtc gagtcgatcg acgacatgcc ggcggcgatc ctggctgccg
     7141 cgcgtcccga cgacgtggtg atcacgatgg gtgcaggctc ggtcggcgcc gtacccgcga
     7201 agctcacgca cgcaggaggt gccgaatgac gatgaccatc gacccgaaga gcttcggtcg
     7261 cgtgggcgtg ctgtttggcg ggcgctcgtc cgagcgggag gtgtcgatca tgtccggcac
     7321 gggcgtgctc gacgcgctga agagccgcgg cgtcgatgcg cacgcattcg atccgggcgc
     7381 gcagtcgctc gcggaactcg aggcggcgcg gttcgaccgc gtgttcatcg cgctgcacgg
     7441 ccgcttcggc gaggacggca cgatccaggg cgtgctggag acgctgcgca ttccgtatac
     7501 gggcagcggc gtgcaggctt ccgccatcgc gatcgacaag ttgactacga agaagatctg
     7561 gctggcggag gaactaccca cgccggcgtg gcgcgtcgtg cacgcgcaca ccgactggat
     7621 gcagctggtt gccgagctcg gcgaccagct gatcgtgaag ccgtcgcgcg aagggtcgac
     7681 gatcggcatc accaaagtga tcggccacga ccgtaatgaa ctcgcgatgg ccttcgaggc
     7741 agcggtgaag tacgacgact gcgtgatcgt ggaggagttg atccgcgggc gcgagttgac
     7801 ctgcgcgatc ctcggcgaag gcgcaaccgc ccgcgcattg cctctgatcg aaatccgggc
     7861 tccggcggag aactacgact accacagcaa gtacttcagc gacgagacga agtacctctg
     7921 tccggcaccg ctggacgatg ttcttgcgga ggaactgcag gatctgtgcg tgcgagccta
     7981 caacgcactg ggtgcgcgcg gctggggccg catcgacctg atgctcaacc aggatccgcg
     8041 cggcgaccgg ccgtatctgc tcgaactcaa cacctcgccg ggcatgaccg gccactcgct
     8101 ggtgccaatg gcggccaagg cgatcggcgt gacctacgag gagctcgtgc tggagatact
     8161 ttcctcggcg cggctcgagg tcccggcgtg aatcgggttc gcacgatgca atgggcgacg
     8221 cgagcgctgc tggcggcgac gggcgtgctc gtgctcagcg cggcggcgct gtgggtcgcg
     8281 cagcggccgc agttcgggat ccggcgcatc gagctgcgtg gcgccgacgg cgccttgcag
     8341 catgtttctg ctgccacagt ccgcgcggcg ctacgcggcg acggccgcac gcggctgcgt
     8401 ggcggctact tcacgctgcg gctcgacgat gcacgccggg tcttcgagac ggtgccctgg
     8461 gtcgcgggcg tgtcggtgcg acgcggctgg cccgatcgtc tggtcgtcac attcaccgag
     8521 catcgggcgc ttggtacctg ggttgacggc cggctgctat cggacgcggg cgttctgttc
     8581 gtcgccaatc cggcggaagc ggacgtcgat ggcgcgctgc cggaattcga cgggccggag
     8641 cgcttcgcac ccgatgtcgc gcgccgcttc cgcgaattca cgtccacgct cgcgccgctg
     8701 cagctcacgg tcgagtcggt cgaggtctcc gagcgagcgg cgtggcgtct gcgcaccgat
     8761 accgagcaga cgatcgagct cgggcgcgac gatcccgcgg gacgtatcgc gcaacggctg
     8821 gccgccttcg tcgccagtta cccgctcgtg ctcgcacgca cacaaggcgc gccgccacgc
     8881 atcgacctgc gctacgccaa cggatttgcg gtggcgctgc cagcaaccca caggaagcca
     8941 tgaagaacga gaacaaggat ctggtcgtcg gactcgatat cggcacctcg aaggtcgtgg
     9001 tcgcggtggc cgaggtgctt cccgacggac gcttccaggt gatcggactc gggcaggcgg
     9061 cctccgaggg actgcgcaag ggcgtggtgg tgaacatcga ggcgacggtg cagtcgatcc
     9121 ggcgcgcgct cgaggaagcg gaactcatgg ccgcgtgccg catcaccaac gtcttcaccg
     9181 gcatcgccgg cagccacatc aagagcttca actcgagtgg catggtggcg atcaaggatc
     9241 gcgaagtcac cgcgagcgac gttgcccggg tgatcgagac cgcgcgcgcg gtcaacatcc
     9301 cgaccgacca gcaggtgctg cacgtgctga cgcaggagtt catcgtcgac gggcaggagg
     9361 acattcgcga gccgatcggc atgagcggag tgcggctcga ggtcaaggtg cacatcgtca
     9421 ccggcgccgt gagcgcggcg cagaacatca tcaagtgcgt gcgccgctgc gggctcgagg
     9481 tgcaggacct gatcctgcag ccgctggcgt cgagtctttc ggttctcacg cccgatgaga
     9541 aggaactggg cgtggcgctg gtcgacatcg gcggcggcac cagcgacat
//
LOCUS       DBNHBY010000217         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_757216, whole genome shotgun sequence.
ACCESSION   DBNHBY010000217 DBNHBY010000000
VERSION     DBNHBY010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_757216"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2030)
                     /locus_tag="AC16HB_05375"
     CDS             complement(<1..2030)
                     /locus_tag="AC16HB_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606256.1"
                     /translation="MPVRLALFGSPSLEHDGGSVALPFERRNQLLAFLALRRSWVGRA
                     ELAALLWPEQESGRAYANLRKTLFRLQALPWAAPIEAQGGAIRFDADTDVHAFDAALR
                     EGRIADALRLRRGELLAGFDDDGNEAWSNWLGFERDRLRAAWRDAALEHLAHDVDPGA
                     AIELSARLLDADPLDEAALRAHMAWLARGGQSARARQTYRDFVRRIDAELGIAPSAEL
                     KALHDSLGAPVAVPAPSTTATAPPAEEGFIGRAVELHRIADLLARGDCRLLCLIGPGG
                     VGKTRLAQRALRELAAGYAGGAAFIPLEDIASAAELGGQLARELGVALTGGSDPFDQV
                     IEFLRERQMLLVLDNFEQLVPAAPALDRLLNACPRLKIVVTSRVRLASSLEWLLPIEG
                     LPCPEMEDQDRIESFDAARLFIRAARRVEPALLPAVEAASIVDICRQVEGLPLALELA
                     AAWTRVLSCEAIAAELRQGSELLSAVDASRPARHASFEVVFEQSWRLLGTAERDALAK
                     LSVFRAGFSSEAALAVARAPLPVLGALADKSLLREEGTRLSLHPLVHQLAAARLGQGA
                     ERDATETEHARYFHRLLVQLRSAVKRCDRDALALFETEFENCRLAWRQSAARDTGAAL
                     LASSALTLLHFCDHRGRFDEGLALMRAALASRIAGADTRLGALLQAAVAHLE"
BASE COUNT          377 a          870 c          827 g          311 t
ORIGIN      
        1 tattccagat gcgccacggc ggcctgcagc agcgcgccca gtcttgtgtc ggcgccggcg
       61 atgcgcgatg caagcgccgc gcgcatcagg gccaggcctt cgtcgaaccg gccacggtga
      121 tcgcagaagt gcagcaaggt gagcgcgctg ctcgccagca gcgccgcacc cgtatcgcgc
      181 gccgccgact gccgccaggc gagccggcag ttctcgaact cggtctcgaa gagtgcgagc
      241 gcatcgcggt cgcaccgctt cactgcgctt ctcagctgga ccagcaggcg atggaagtag
      301 cgcgcatgct cggtctccgt cgcgtcacgc tccgcgccct ggccaaggcg agccgcggcg
      361 agctgatgca ccagagggtg gagcgacaga cgcgtcccct cctcgcgcag cagcgacttg
      421 tcggccagcg caccgagcac ggggagcggc gcgcgggcga cggcgagcgc ggcctcggaa
      481 gagaacccgg cgcgaaacac cgacagtttc gcgagtgcat ctcgctccgc cgtaccgagc
      541 aggcgccacg actgctcgaa caccacctcg aagctcgcgt gccgcgccgg gcgggacgcg
      601 tcgaccgcgc tcagcagttc acttccctgc cgcaactcgg cggcgatcgc ctcgcacgaa
      661 agaacgcggg tccaggcggc agccagctcc agcgccagcg gcagcccctc gacctggcga
      721 cagatgtcga cgatcgaggc cgcttcgacc gcgggcagca gtgccggctc gacccgccgc
      781 gcggcgcgaa tgaacaggcg cgcggcatcg aacgactcga tgcggtcctg atcctccatt
      841 tcggggcacg gcagtccctc gatcggcagc agccactcca gtgaactggc cagccgcacg
      901 cgcgacgtga cgacgatctt gaggcgtgga cacgcattca ggagtcgatc gagagccggc
      961 gctgcgggca ccagttgctc gaagttgtcg agcaccagca gcatctgccg ttcgcgcagg
     1021 aattcgatca cctggtcgaa cggatcactg ccgccggtga gggcgacgcc gagttcgcgc
     1081 gccagctggc cgcccaactc ggcggccgag gcgatgtctt cgagcgggat gaacgccgcg
     1141 ccgcccgcat agcctgccgc aagctcccgc aacgcgcgct gagccaggcg cgtcttgccg
     1201 acgccgcccg ggccgatcag gcacagcagc ctgcagtcgc cgcgcgccag cagatcggcg
     1261 atccggtgca gctcgaccgc gcggccgatg aatccctcct cggccggcgg cgcggttgcc
     1321 gtcgtcgacg gcgccggcac ggcgaccggc gcgccgagcg agtcgtgcag cgccttcagc
     1381 tcggcgctcg gcgcaatgcc gagttccgcg tcgatgcgcc ggacgaaatc gcggtaggtc
     1441 tggcgcgcgc gcgcactctg cccgccgcgt gccagccatg ccatgtgtgc gcgcagcgcg
     1501 gcctcgtcga gcgggtcggc gtcgagcagc cgtgccgaca gctcgatcgc cgcgccgggg
     1561 tcgacgtcgt gcgccaggtg ttcgagcgcg gcgtcgcgcc acgccgcacg cagccggtcg
     1621 cgctcgaagc cgagccagtt cgaccaggcc tcgttgccgt cgtcgtcgaa cccggcgagc
     1681 agctcgccgc ggcgcaggcg cagcgcgtcg gcgatccggc cctcccgcag cgccgcgtcg
     1741 aaggcgtgca cgtcggtatc ggcgtcgaag cggatcgcgc cgccctgcgc ttcgatcggc
     1801 gcagcccacg gcagcgcctg caggcgaaac agcgtcttgc ggaggttggc gtaggcgcgg
     1861 ccgctttcct gctcgggcca cagcagtgcc gccagctccg cacgaccgac ccacgaccgc
     1921 ctgagcgcga gaaacgcaag cagctggttg cgccgctcga acggcagcgc gaccgacccg
     1981 ccgtcgtgct cgagcgacgg cgagccgaac aaggcaagac gaaccggcat ctcctctcct
     2041 gagcgggcgc acagcatgcg cgagtccgct gcgtgcggca atcgcgtgga acgctggtgc
     2101 aacgggcacg tgcttatggt cccgtccttc ggagcgacgg cagcgcgctc gaaggcagca
     2161 cgggaatgtt ggcacgcgcc ggagccgaca tgagttcagc ggccgaaacg ccggcgataa
     2221 ctgcggcgcg ccgcttcgcc caggtcgcct cccacgatcg gtgccggcgc cgctgttcgc
     2281 cgacggcgcg ttcaacagcg tgctgtcgac cgggcggcac cgaggaacgc gccgatcgtc
     2341 cgcaacgtca tgcagtcaag gagtcattgc catgcatcgt cttca
//
LOCUS       DBNHBY010000218         1903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1839573, whole genome shotgun sequence.
ACCESSION   DBNHBY010000218 DBNHBY010000000
VERSION     DBNHBY010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1839573"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="AC16HB_05380"
     CDS             <1..1535
                     /locus_tag="AC16HB_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5606257.1"
                     /translation="PALSCDPEFGGQGLPITLNQCLYEMLNSANQAWTMYPGLSHGAY
                     EALHEHGTEEQKKTYLPKLVSGEWTGTMCLTEAHCGTDLGMLRTKAEPQPDGSHRLTG
                     EKIFISAGEHDLADNIVHLVLGRLPDAPAGTKGISLFVVPKFVPQGKGADARVGARNK
                     LHCGGIEEKMGIHGNATCQIILDGATGWLVGEPNKGLNAMFVMMNGARLAVGVQGLGI
                     TEAAYQNAVAYARDRIQGRSLTGAKQPDKPADPIIVHADVRRMLLTARAYAEGGRAFA
                     YWVALQADKQLHHPDEESRKFAADMMALMTPVVKAFLTDNGYQCATECMQVLGGHGYV
                     RANGMEQLVRDARINMIYEGTNGIQSLDLIGRKVLMDNGAKLRKFGKLVQDFIEAHGT
                     STEMNEFVTPLADLGDKVTKLTMELGMKAFQNQDEAGAAATSYLRVIGHLCLAYFWAR
                     MARVALTRVKQDGDEVDPFYTAKLTVARFYFQRLLPETAYHMRAARSGAKNVMELPAE
                     MF"
     gene            1562..>1903
                     /locus_tag="AC16HB_05385"
     CDS             1562..>1903
                     /locus_tag="AC16HB_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR5606258.1"
                     /translation="MTNKNFIVRKVAVLGAGVMGAQIAAHCVNAKVPVVLFDLPAKEG
                     PKNGIVLKAIDNLKKLSPAPFASKDDAVHIEPANYEEHLELLKGCDVVIEAIAERMDW
                     KHDLYKKVAPYI"
BASE COUNT          382 a          601 c          645 g          275 t
ORIGIN      
        1 ggccggcgtt gtcgtgcgac ccggagttcg gcggccaggg cctgccgatc accctcaacc
       61 agtgcctcta cgagatgctc aatagcgcga accaggcgtg gacgatgtat ccgggcctct
      121 cgcatggcgc gtacgaggcg ctgcacgagc acggcaccga ggagcagaag aagacctacc
      181 tgcccaagct ggtgtccggc gagtggaccg gcacgatgtg cctgaccgag gctcactgcg
      241 gcaccgacct cggcatgctg cgcacgaagg ccgagccgca acccgacggc agccacaggc
      301 tcaccggcga gaagatcttc atctccgccg gcgagcacga cctggcagac aacatcgtgc
      361 acctcgtgct ggggcggctg cccgatgcgc cggcgggcac caagggaatt tcgctgttcg
      421 tggtgccgaa attcgtaccg cagggcaaag gtgccgacgc cagggtcggc gcgcgcaaca
      481 agctgcactg cggcggcatc gaggagaaga tgggaattca cggcaacgcg acctgccaga
      541 tcatcctcga cggcgcgacc ggctggctgg tcggcgagcc gaacaagggc ctgaatgcga
      601 tgttcgtgat gatgaacggc gcgcggctcg cggtcggcgt acagggactc ggcatcaccg
      661 aggcggccta ccagaacgcg gtcgcgtacg cgcgcgatcg catccagggc cggtcgctga
      721 cgggcgcgaa gcagcccgac aagccggccg atccgatcat cgtgcacgcc gacgtgcggc
      781 gcatgctcct gaccgcgcgc gcgtacgcgg aaggcggacg cgcattcgcc tactgggtcg
      841 cgctgcaggc tgacaagcag ttgcaccacc cggacgagga gagccgcaag ttcgccgccg
      901 acatgatggc gctgatgacg ccggtggtga aggccttcct caccgacaac ggctaccagt
      961 gcgcgaccga gtgcatgcag gtgcttggcg gccacggcta cgtccgtgcc aacggcatgg
     1021 agcagctggt gcgcgacgcg cgcatcaaca tgatctacga gggcaccaac ggcatccagt
     1081 cgctcgacct gatcggccgc aaggtgctga tggacaacgg tgccaagctg cgcaagttcg
     1141 gcaagctggt gcaggacttc atcgaggcgc acggcacgtc gaccgaaatg aacgagttcg
     1201 tcacgccgct ggcggacctc ggcgacaagg tgacgaagct cacgatggaa ctcggcatga
     1261 aggcgttcca gaaccaggac gaagccggcg cggccgcgac cagctacctg cgcgtgatcg
     1321 gtcacctgtg cctggcctac ttctgggcgc gcatggcgcg cgtggcgctg acgcgcgtga
     1381 agcaggacgg tgacgaggtc gatccgttct acaccgccaa gctcacggtc gcccgcttct
     1441 atttccagcg cctgctgccg gagaccgcgt atcacatgcg tgcggcacgc tccggcgcga
     1501 agaacgtgat ggaattgccc gcggaaatgt tctgaagagc gtccggccag gagagaaagt
     1561 gatgaccaac aagaatttca tcgtccgcaa agtcgccgtg ctcggcgccg gcgtgatggg
     1621 cgcgcagatc gccgcgcatt gcgtgaatgc caaggtgccg gtggtgctgt tcgacctgcc
     1681 ggcgaaggaa ggaccgaaga acggcatcgt gctgaaggcg atcgacaacc tcaagaaact
     1741 gagtcccgcg ccgttcgcta gcaaggacga cgcggtccac atcgagcccg cgaactacga
     1801 ggagcacctc gagctgctca agggctgcga tgtggtgatc gaggcgatcg ccgagcgcat
     1861 ggactggaag cacgacctgt acaagaaggt cgcgccatat atc
//
LOCUS       DBNHBY010000219         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1501338, whole genome shotgun sequence.
ACCESSION   DBNHBY010000219 DBNHBY010000000
VERSION     DBNHBY010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1501338"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16HB_05390"
     CDS             <1..261
                     /locus_tag="AC16HB_05390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5606259.1"
                     /translation="GTFTQAMFVLPNSRENEQEADRIGVELAARAGYDPRAAVSLWNK
                     MSQLGGSSTPQWLSTHPSHETRVRDLQDYSQRVLPLYEKARR"
     gene            complement(258..926)
                     /locus_tag="AC16HB_05395"
     CDS             complement(258..926)
                     /locus_tag="AC16HB_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5606260.1"
                     /translation="MRPNNLPTLRRRRALASIGALCLAPALAGAQINRERRTTPAQTL
                     GPFYPRSAAERPAQTDNDLLVLDGDRVLTKGVPLYLTGHVVSKLGPIADARVEIWQCD
                     ANAVYHHPAGGAEGDRDRSFQGYGETRTDGSGAFHFRTIRPVPYPGRTAHIHVRVEAN
                     GLPPLSTQLYLADEAGNARDFLYRQLNEAERTAVTLSPRPTASTHPLARGTRVTADVE
                     IVVA"
     gene            1022..>1842
                     /locus_tag="AC16HB_05400"
     CDS             1022..>1842
                     /locus_tag="AC16HB_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5606261.1"
                     /translation="MPQTLYDKLWDAHVVHVENDGTTILYVDRHLVHEVTSPQAFEGL
                     RLAGRTPWRMTSVVATADHNTPTKGWERGYDGIVDPISREQIVTLDANIRQYGAAAYF
                     PFLDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAFGIGTREVEHVLATQ
                     TLLRKKAKNMLVKVDGKLPFGCTAKDIVLAVIGRIGTAGGTGCAIEFAGSTIESLSME
                     GRMTVCNMAIEAGARAGMVAVDDRTIEYLRGRPFAPQGEAFDEAARYWRTLKSDPG"
BASE COUNT          310 a          558 c          687 g          287 t
ORIGIN      
        1 ggcactttca cacaggcgat gttcgtgctg ccgaacagtc gcgagaatga gcaggaggcc
       61 gaccgcatcg gcgtggaact cgccgcgcgc gccggctacg atccgcgcgc cgcggtcagc
      121 ctgtggaaca agatgtcgca actgggtggc agctcgacgc cgcagtggct gtcgacgcac
      181 ccgtcgcacg agacgcgggt gcgcgacctg caggactatt cgcagcgcgt gctgccgctg
      241 tacgagaagg cgcggcgcta ggccacgacg atttcgacgt ctgccgtaac gcgcgtaccg
      301 cgcgccagcg ggtgggtcga tgcggtcggc cttggcgaca gcgtgaccgc cgtgcgctcg
      361 gcctcgttca gttgccgata cagaaagtcg cgcgcgttgc cagcctcgtc ggcaaggtag
      421 agctgggtcg acagcggcgg caagccgttc gcttcgacgc gtacgtgaat gtgcgccgtg
      481 cggcccggat acgggaccgg acggatcgtg cggaagtgga acgcgccgga accgtcggtg
      541 cgcgtctcgc cgtagccctg gaagctgcgg tcgcggtcgc cctcggcgcc accggcggga
      601 tggtggtaga cggcgttggc gtcgcactgc cagatctcca cgcgcgcgtc agcgatcggg
      661 ccgagtttcg atacgacgtg gccggtcagg taaagcggca cgcccttggt cagcacgcga
      721 tcgccgtcga gcaccagcag gtcgttgtcg gtctgcgccg ggcgctcggc ggccgagcgc
      781 ggatagaacg ggccgagcgt ctgcgccggc gtggtgcgac gctcgcgatt gatctgcgcg
      841 ccggccagcg ccggcgcgag gcagagcgcg ccgatcgagg cgagcgcacg gcggcggcgg
      901 agcgtgggca gattgttggg tcgcatgcga tggcctctcg cagtggtcga ttgcgctatt
      961 aaggcgcgcg gcgaaattcg ttccgcgtcg cggcataatt gcatttctag ccggaaagat
     1021 catgccgcaa acgctgtacg acaagctgtg ggatgctcat gtggtgcacg tcgagaacga
     1081 cgggaccacg attctctacg tcgatcgcca tctggtgcac gaagtgacgt cgccgcaggc
     1141 gttcgaaggg ctgcggctcg ccggccgcac gccgtggcgc atgacgtcgg tcgttgcgac
     1201 cgccgatcac aacacgccga ccaagggctg ggagcgcggt tacgacggca tcgtcgatcc
     1261 gatcagcagg gagcagatcg tcacgctcga cgcgaatatc cggcagtacg gcgcggcggc
     1321 gtatttcccg tttctggaca agcgacaggg catcgtgcac gtgatcgggc cggagcaggg
     1381 cgcgacgctg ccgggcatga cggtcgtgtg cggtgattcg cacaccagca cgcatggtgc
     1441 gttcggtgcg ttggcgttcg gcatcggcac cagagaagtc gagcacgtgc tggctacgca
     1501 gacgctgctg aggaagaagg cgaagaacat gctggtgaag gtcgacggca agctgccgtt
     1561 cggctgcacc gcaaaggaca tcgtgctcgc ggtgatcggc aggatcggca cggccggcgg
     1621 cacgggctgt gcgatcgagt tcgccggcag cacgatcgag tcgttgtcga tggaagggcg
     1681 aatgaccgtc tgcaacatgg cgatcgaggc gggcgcgcgc gccggcatgg tcgcggtcga
     1741 cgacaggacg atcgagtacc tgaggggccg gccgttcgcg ccgcagggcg aggcgttcga
     1801 cgaagcggca cgctactggc gcacgctgaa gagcgatccg gg
//
LOCUS       DBNHBY010000220         2349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1501342, whole genome shotgun sequence.
ACCESSION   DBNHBY010000220 DBNHBY010000000
VERSION     DBNHBY010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1501342"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="AC16HB_05405"
     CDS             <1..1043
                     /locus_tag="AC16HB_05405"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606262.1"
                     /translation="VLMADVTGIDTAARAVQLDDGQRIAYDQLIVATGATHSYFGHAE
                     WQAHAPGLKTLEDAFDVRRRILVAFEQAEREPDDAARAAWLTFIVIGAGATGVEMAGM
                     LAEIARHTLSGEFRRFDSRNARVILLEGGPRVLPAFPEDLSQRARVQLEKIGVTVWLD
                     SRVTAIDERGVEIGGTRIDARTIIWAAGVEASSLGRALAVPLDRAGRVLVEPDLSIPG
                     HPEVFVIGDLAAVTTDGKPVPGVSPAAKQMGRHTARNIVRRLRGEPTLPFRYRDYGTL
                     ATIGRKAAVAILGPLKLSGYPAWLTWLFAHVYFLIGFRNRLVVLLDWAWAYWSFTRFA
                     RVVIEPMRAAKD"
     gene            complement(1050..1586)
                     /locus_tag="AC16HB_05410"
     CDS             complement(1050..1586)
                     /locus_tag="AC16HB_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5606263.1"
                     /translation="MLIRKIHHVAYRCRDAKQTVEWYEKHLGMKLVLAIAEDEVPSTR
                     EPDPYMHIFLDAGGGNILAFFELPTRPPMGRDASTPTWVQHFAMEVDSVDTLLATKAK
                     LEAAGIEVVGPTDHTLFKSIYFFDPNGLRLELAANTATPEMMEKLDAVKWDMIDEWSR
                     TRKAPKHAAWLHDGSAAR"
     gene            complement(1623..1847)
                     /locus_tag="AC16HB_05415"
     CDS             complement(1623..1847)
                     /locus_tag="AC16HB_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014619268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HIR5606264.1"
                     /translation="MPLQLSPNFAEPGRRYVRDFSPGDDFYQALIDTHRDLSDEQSAL
                     LNAKLVLLLANHIGDIGLLREALALAREGV"
     gene            complement(1858..>2349)
                     /locus_tag="AC16HB_05420"
     CDS             complement(1858..>2349)
                     /locus_tag="AC16HB_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5606265.1"
                     /translation="ARRHAFARKLVNSGRLSVPAFYVDSSLNTPDEDTFSSHLVPGAP
                     AEDAPVGVDGRDGWLIDQVGNRFQLLVYVDDAARFDLARFAALRDAPIPVETIVVSPR
                     AAIRSGVRVLHDRKDRFAQRFDARDGAAYLLRPDQHLAARWRAFDAGKVRAAVMRATC
                     NAL"
BASE COUNT          355 a          801 c          801 g          392 t
ORIGIN      
        1 ccgtgctgat ggccgacgtg accggaatcg acaccgccgc gcgtgccgtg cagctcgacg
       61 acgggcagcg catcgcctac gaccagctga tcgtcgcgac cggcgccacg cacagctact
      121 tcgggcacgc ggaatggcag gcacacgcgc cggggctcaa gacgctggag gatgcgttcg
      181 acgtccgccg ccgcatcctc gtcgccttcg agcaggcgga gcgcgagccc gacgatgccg
      241 cgcgtgctgc gtggttgact tttatcgtga tcggcgccgg cgccaccggc gtcgagatgg
      301 ccggcatgct ggccgaaatc gcgcgccata cgctgagcgg cgagttccgc cgcttcgatt
      361 cgcgcaatgc gcgcgtgatc ctgctcgaag gtgggccgcg cgtactgccg gcgtttcccg
      421 aggatctgtc tcagcgcgcc cgggtgcagc tcgaaaagat cggcgtcacg gtctggctcg
      481 acagccgcgt caccgcgatc gacgaacgcg gcgtcgagat cggcggcacg cgcatcgacg
      541 cgcggacgat catctgggcc gcgggggtcg aagcctcgtc gctcggccgt gcgctcgccg
      601 tgccgctgga ccgtgcaggc cgcgtgctgg tcgaacccga tctgtcgatc cccggccacc
      661 ccgaggtctt cgtgatcggc gacctcgccg cggtgacgac cgacggcaag ccggtgccgg
      721 gcgtcagccc ggccgccaaa cagatggggc gccacacggc gcgcaacatc gtgcgccggc
      781 tgcgcggcga accgacgctg ccgttccgct atcgcgacta cggcacgctg gcgacgatcg
      841 gccgcaaggc ggccgtcgcg atcctcgggc cgctgaagct gtcaggctat ccggcgtggc
      901 tgacgtggct gttcgcgcac gtctatttcc tgatcggatt ccgcaatcgc ctcgtggtgc
      961 tgctcgattg ggcgtgggcg tactggagct tcacgcgctt tgcgcgcgtg gtgatcgagc
     1021 cgatgcgtgc tgcgaaggac tgagtcgcgt cagcgcgcag cactgccgtc gtgcagccac
     1081 gccgcgtgct tgggcgcctt ccgggtgcgc gaccactcgt cgatcatgtc ccatttcacc
     1141 gcgtcgagct tctccatcat ctccggcgtg gcggtgttgg cggcgagctc cagccgcagg
     1201 ccgttgggat cgaagaagta gatcgacttg aacagcgtgt ggtcggtcgg gccgacgacc
     1261 tcgatgcctg ccgcttcgag cttcgccttc gtggcgagca gcgtgtcgac cgaatcgacc
     1321 tccatcgcga aatgctgcac ccaggtcggc gtgctggcgt cgcggcccat cggcggcctg
     1381 gtcggcagct cgaagaacgc caggatgttg cccccgccgg cgtcgaggaa gatatgcatg
     1441 tacggatcgg gttccctggt cgacggcacc tcgtcctccg caatcgccag caccagtttc
     1501 atgccgaggt gcttctcgta ccactcgacg gtctgcttgg cgtcgcggca gcgataggca
     1561 acgtgatgaa tcttgcggat cagcatggcg agctccgttt ctttctgcac gtccgacgac
     1621 ttttataccc cttcgcgcgc cagcgccagc gcctcgcgca gcaggccgat gtcgccgatg
     1681 tgattcgcga gcagcaacac cagcttggca ttcagcagcg cgctctgctc gtcgctgagg
     1741 tcgcgatggg tatcgatcag cgcctgatag aagtcgtcgc ccggcgagaa gtcgcgcacg
     1801 taacggcgcc ccggctcggc aaagttggga ctcaattgca gcggcatcgt cgtcctttca
     1861 gagcgcgttg caggtcgcac gcatgaccgc ggcgcgtacc ttgccggcgt cgaacgcgcg
     1921 ccagcgcgcg gcaaggtgct gatcgggccg cagcaggtac gcggctccgt cgcgcgcatc
     1981 gaatcgctgc gcgaagcggt ccttgcgatc gtgcagcacg cgcacgccgg accgtatcgc
     2041 cgcgcgcggc gatacgacga tcgtctcgac cgggatcggc gcgtcgcgca gtgccgcgaa
     2101 ccgcgcaagg tcgaaccgcg cagcgtcgtc cacgtacacg agcagctgga agcggttgcc
     2161 gacctgatcg atcagccagc cgtcgcggcc gtcgacgccg accggcgcgt cctcggccgg
     2221 cgcgcccgga accagatgcg acgagaaggt gtcttcgtcc ggggtgttca gggacgaatc
     2281 gacgtagaac gcgggcaccg acagtcgccc cgagttcacc agcttgcgcg cgaacgcgtg
     2341 cctgcgcgc
//
LOCUS       DBNHBY010000221         2131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1365920, whole genome shotgun sequence.
ACCESSION   DBNHBY010000221 DBNHBY010000000
VERSION     DBNHBY010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1365920"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC16HB_05425"
     CDS             <1..428
                     /locus_tag="AC16HB_05425"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5606266.1"
                     /translation="EFNLPWVAKAADAPVAIEVNAWILVHPDDRVVIRIARSEMGQGT
                     LTALAQLVAEELDCDWSNVSTEFASPNEHVRRNRVWGSMSTGGSQGVRGSHDYVRKAG
                     ASAREMLIAAAAARWQVAPSECTVERGVVSHRPSGRTLR"
     gene            474..>2131
                     /locus_tag="AC16HB_05430"
     CDS             474..>2131
                     /locus_tag="AC16HB_05430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5606267.1"
                     /translation="MKLRDPKDWKIAGRAVHRLDIPDKVLGKPVFAVDVSLPGMLHAS
                     IAQCPVFGGQVASVDAREAERMRGVRKIVRESNFVAVVADNWWRANEALKKVKIQRDA
                     AGNGDASNETIDTMLRDGLAQAALPKARELGNVASALASAAKVIEAEYRSPYLNHATL
                     EPQTCTAWLRPDGFLEVWTSTQNGEASLSAAAETAGLPLERVEVHKMMLGGGFGRRGA
                     PQDFVRQGVAIAKALPGVPVKLMWSREEDMQHGFYRPASIVRMRAGLDAKGNLIALHT
                     KIACPSIAESFARYNVPATNSVIAAGRSIARGLMSQVTPGDRIDSSAVRAFSDLPYAV
                     PNQQVDYAQRNGHVPVGYWRAPGQQNEFYRECFIDELALAAGRDPVDYRLALLKEGDK
                     HRLVLQAAAKAAGWATPPPPGVHRGVALAYGFGSYAAMVAEVSVSAQGEPKVHRIVVA
                     IDSGHVVNPDTCRAQAESNVVFGLGAVLNQQINVRNGRVVESNFHDFPLPTIADMPQV
                     ETLLVPSGGFWGGHGEPAILPLAPAVCNAVSRATGRRIRALPLK"
BASE COUNT          366 a          707 c          756 g          302 t
ORIGIN      
        1 tcgagttcaa cctgccgtgg gtggcgaagg ctgcggacgc accggtcgcc atcgaagtca
       61 acgcctggat cctggtccat cccgacgacc gcgtggtgat ccgcatcgcg cgctcggaga
      121 tgggacaggg caccctgacc gcgctcgcgc aactggtcgc ggaggaactg gactgcgact
      181 ggtccaacgt cagcacggag ttcgcgtcgc ccaacgagca cgtccggcgt aaccgcgtct
      241 ggggctcgat gtcgaccggc ggcagccagg gcgtgcgtgg gtcgcacgac tacgtgcgca
      301 aggccggtgc gagcgcgcgc gagatgctga tcgcggccgc ggccgcacga tggcaagtcg
      361 cgccgtcgga gtgcacggtc gagcgcggag tggtttcgca ccgaccgtcg ggccgcacgc
      421 tgcgctaagg tcaggtcgca gcggcggcag cgaagctgac gccaccgcgc gaggtgaagc
      481 tgcgcgaccc aaaggactgg aagatcgcgg gccgagccgt gcaccgcctc gacattcccg
      541 acaaggtgct cggcaaaccg gtattcgcgg tcgacgtatc gttgccgggc atgctgcacg
      601 cgtcgatcgc ccagtgtccg gtgttcggcg gccaggtcgc aagcgtcgac gcgcgcgagg
      661 cggaaaggat gcgcggcgtc aggaagatcg tgcgcgagtc gaacttcgtc gccgtcgtcg
      721 ccgacaactg gtggcgcgcg aacgaggcgc tgaaaaaagt gaagatccag cgggacgccg
      781 ccggcaacgg cgacgccagc aacgaaacga tcgacaccat gctgcgcgac ggcctggcgc
      841 aggcggcgct gccgaaagcg cgcgaactcg gcaacgtggc gtcggcgctc gcgtccgccg
      901 ccaaggtgat cgaggccgaa taccgttcgc cctacctgaa ccatgccacg ctggagccgc
      961 agacgtgcac tgcgtggttg cggcccgacg gcttcctgga agtgtggacg tcgacgcaga
     1021 acggcgaggc gtcgctgtcg gccgcagcgg aaaccgcggg cctcccgctc gaacgggtcg
     1081 aggtgcacaa gatgatgctc ggtggcggtt tcggccgccg cggggcaccg caggatttcg
     1141 tccgccaggg cgtggcgatc gcaaaggcgc tgcccggcgt gccggtcaag ctgatgtggt
     1201 cgcgcgagga ggacatgcag cacggcttct atcgcccggc gagcatcgtg cgaatgaggg
     1261 ccggcctcga cgccaagggc aacctgatcg cgctgcacac gaagatcgcc tgcccatcga
     1321 tcgccgagtc cttcgcgcgg tacaacgtgc cggcgacgaa ctccgtgatt gcagcgggaa
     1381 ggtcgatcgc ccgcggcctg atgtcacagg tcacgccggg agaccggatc gactcctcgg
     1441 cggtgcgcgc attcagcgac ctgccctacg cggtgccaaa ccagcaggtc gactacgcgc
     1501 agcgcaacgg ccacgtgccc gtcggctact ggcgtgcgcc ggggcaacag aacgagttct
     1561 atcgcgagtg cttcatcgac gaactcgcac tcgccgcggg cagggatccc gtcgactacc
     1621 ggctcgcgct gctgaaggaa ggcgacaaac accgcctcgt gttgcaggcc gcagcgaagg
     1681 ccgccggctg ggcaacgccg ccgccgcccg gcgtccaccg gggtgtcgcg ctcgcgtacg
     1741 gattcggaag ctatgctgcg atggtcgcgg aagtctcggt gagcgcgcag ggcgaaccga
     1801 aggtccaccg catcgtcgtc gcgatcgact cgggccacgt ggtcaatccc gacacctgcc
     1861 gtgcccaggc tgagagcaac gtggtgttcg ggctcggcgc ggtgctgaac cagcagatca
     1921 acgtcaggaa cggccgcgtc gtggaatcga acttccatga cttcccgctg ccgacgatcg
     1981 ccgacatgcc gcaggtcgag acattgctcg tgcccagcgg cggtttctgg ggcggccatg
     2041 gcgagccggc gatcctgccg ctggcaccgg cagtctgcaa cgccgtgtcg cgcgccaccg
     2101 gcaggcgcat ccgtgcgctg cctctgaagc a
//
LOCUS       DBNHBY010000222         5160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1568931, whole genome shotgun sequence.
ACCESSION   DBNHBY010000222 DBNHBY010000000
VERSION     DBNHBY010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1568931"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16HB_05435"
     CDS             complement(<1..347)
                     /locus_tag="AC16HB_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5606268.1"
                     /translation="MTAQFDPIDPPPALAPATDRIDLADPAFISDLHLNADRPRTLDA
                     FLRFMREIAPGHKELVILGDLFEYWAGDDDLADPVAQVVCGALAEHAHQNRRVFVMHG
                     NRDLLLGRGFAQHA"
     gene            complement(350..841)
                     /locus_tag="AC16HB_05440"
     CDS             complement(350..841)
                     /locus_tag="AC16HB_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5606269.1"
                     /translation="MVRLHTNHGVITLELDEAKAPKTVANFLAYVKSGHFGNTLFHRV
                     INGFMIQGGGFEPGMKQKPTSKPVENEAGNGLRNDKYTIAMARTSDPHSATAQFFINV
                     ADNQFLNHTAPTGQGWGYCVFGKVVDGFETVDKIKAVRTTRFGMHQDVPEQDVVIERA
                     EIV"
     gene            complement(857..1429)
                     /locus_tag="AC16HB_05445"
     CDS             complement(857..1429)
                     /locus_tag="AC16HB_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5606270.1"
                     /translation="MILSRRGFAAALLALALPAFAQSEPAPRVTLETTEGNIVLELAA
                     DKAPKTVANFLAYVKSGHYDGTIFHRVIDGFMIQGGGYTADLAQKPTRAPIPLESQNG
                     LKNDRGTIAMARTSVPDSATSQFFINVANNDRLNFPSFDGHGYAVFGRVVEGMDVVDK
                     IKSAATAASGPHQNLPVKPIVIRAARIIAK"
     gene            complement(1452..1994)
                     /locus_tag="AC16HB_05450"
     CDS             complement(1452..1994)
                     /locus_tag="AC16HB_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606271.1"
                     /translation="MLLVVAALALAPGAPAQVVSEPPSTVPVPQSAAAEIAALMGKQQ
                     WEPALSRADAFLARNPRDAQVRFQRGVILGELARPQDAIAVFEALTQDYPELPEPYNN
                     LGVLYAAQGNYEAAREQLRKSILAHPGYVTAYENLGDLYVAMAVASYQQAATLDPKNR
                     TAPAKLALARELGTRVRAVR"
     gene            2179..3603
                     /gene="cysS"
                     /locus_tag="AC16HB_05455"
     CDS             2179..3603
                     /gene="cysS"
                     /locus_tag="AC16HB_05455"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5606272.1"
                     /translation="MTLTIYNTLTRQQAPLTPLEPGRVRLYVCGMTIYDFCHLGHARL
                     MMTFDVVVRWLRQRGYEVTYVRNITDIDDKIIKRAAERGITTTALTEEFARYMHEDFD
                     ALGILRPDAEPRATQFVPQMLGLIELLEKKGLAYQDDPDGDVNFAVRRFAGYGKLSGK
                     SLDDLRAGERVAVGERKRDPLDFVLWKHAKAGEPQWASKWGPGRPGWHIECSAMASEV
                     LGRTFDIHGGGPDLIFPHHENEIAQSEGAYEQAFANVWMHCGALRVGEDKMSKSLGNF
                     FTIRDVLRQYGEKWGTARAAEVVRFFLVRGHYRSQIAYDESLLADARSALDRLYIALR
                     DTPPAAGAIDWNESHAQRFAAAMDDDFNTALAVSVLFDLANEVNRGRSPEMARQLKAL
                     GATLGLLQSEPQKYLQGRDVAVHLEGSAAQVEAGRIEALIAQRAAAKKDKNFAEADRI
                     RAELAVQGIALEDRPGGKTLWRRQ"
     gene            3609..4247
                     /locus_tag="AC16HB_05460"
     CDS             3609..4247
                     /locus_tag="AC16HB_05460"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5606273.1"
                     /translation="MQPPYWAQAKRALAKSDPVLARIIRRHPRVALASRGDAFSTLAR
                     SIVGQQISVKAADAVWRRLLGACPELTPPAVLRKRAATLRNCGLSERKVEYLRDLAVH
                     FAKGRVDATAMQAMSDEAVIECLTDIRGIGRWTAEMFLIFNLLRPNVLPLDDLGLLRA
                     ISLNYLDEMPTENLLRREGRAHVERLAAPWAPWRSVATWYLWRSLDPVPVEY"
     gene            4247..>5160
                     /locus_tag="AC16HB_05465"
     CDS             4247..>5160
                     /locus_tag="AC16HB_05465"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR5606274.1"
                     /translation="MAKTTFLEFEQPIAELEAKIEELRFVQDDSAVDISEEIERLQKK
                     SQGLIKDIYAKLTPWQVAQLARHPQRPYTLDYVNDIFTDFHELHGDRAFADDMSIVGG
                     LARFNGHSVMVLGHQKGRDTKERTLRNFGMSKPEGYRKALRLMKLAEKFGVPIFTFVD
                     TPGAYPGVDAEERGQSEAIGHNLFEMSHLEVPIVATIIGEGGSGGALAIAVADHVLML
                     QYATYSVISPEGCASILWKSAEKAPEAAEALGLTAHRLKALGVIDKIVSEPLGGAHRD
                     PKQMAALLKRALGDSLRQFQGMKTKDLLA"
BASE COUNT          879 a         1648 c         1772 g          861 t
ORIGIN      
        1 gcatgctgcg cgaagccgcg cccaagcagc aggtcgcggt tgccgtgcat cacgaacacg
       61 cgccggttct ggtgtgcatg ttcggcaagc gcgccgcaga cgacctgggc gaccggatcg
      121 gcaagatcat cgtcgccggc ccagtactcg aacaggtcgc cgaggatcac caactccttg
      181 tggcccggcg cgatctcgcg catgaagcgc aggaacgcgt cgagcgttcg cgggcgatcg
      241 gcattcaggt gcaggtcgga gatgaaggcg ggatcggcca ggtcgatgcg atccgtcgcc
      301 ggtgccagcg ccggcggcgg atcgatcggg tcgaactgcg ccgtcacgct cagactatct
      361 cggcgcgttc gatcaccacg tcctgctcgg gcacgtcctg gtgcatgccg aagcgcgtgg
      421 tgcggacggc cttgatcttg tcgaccgtct cgaagccgtc gaccaccttg ccgaacacgc
      481 agtagcccca gccctgaccc gtcggtgcgg tgtggttcag gaattggttg tcggcaacgt
      541 tgatgaagaa ctgcgcggtc gccgaatgcg gatccgacgt gcgcgccatc gcgatcgtgt
      601 acttgtcgtt gcgcaggccg ttgccggctt cgttctcgac gggcttcgac gtcggcttct
      661 gcttcatgcc gggctcgaag ccgccgccct ggatcatgaa gccgttgatc acgcggtgga
      721 acagcgtgtt accgaagtgg ccggacttga cgtaagcgag gaagttggcg accgtcttcg
      781 gcgccttggc ctcgtcgagt tccagcgtga tgacgccgtg gttggtgtgc aggcgaacca
      841 tgaatgctcc cgtgggttac ttggcgatga ttctggcggc ccggatcacg atcggcttga
      901 cgggcaggtt ctggtgcggg ccggacgcgg ccgtggcggc ggacttgatc ttgtccacca
      961 cgtccatgcc ctcgaccacg cggccgaaca ccgcgtagcc gtggccgtcg aagctcggga
     1021 aattgaggcg atcgttattg gcgacgttga tgaagaactg cgaggtggcc gagtccggca
     1081 cgctggtgcg cgccatcgcg atcgtgccgc ggtcgttctt caggccgttc tggctctcga
     1141 gcggaatcgg cgcgcgggtc ggcttctgcg cgagatccgc ggtgtagccg ccgccctgga
     1201 tcatgaagcc gtcgatcacg cgatggaaga tcgtgccgtc gtagtgcccg ctcttcacgt
     1261 atgcgaggaa attggccacg gtcttcggcg ctttgtcggc agcgagttcg agcacgatgt
     1321 tgccttcggt ggtttcgagc gtcacgcgcg gcgcgggctc cgattgcgcg aacgccggca
     1381 gcgccagcgc gagcagtgcg gcggcaaagc cgcgacggga aaggatcatt ctctgaaact
     1441 cctgcgcaag gtcaacggac cgcgcgaacg cgcgttccca gttcgcgcgc cagcgcgagt
     1501 ttggccggcg cggttcgatt cttcggatcg agggtcgccg cctgctggta gctggcgacc
     1561 gccatcgcca cgtacaggtc gccgaggttc tcgtacgcgg tcacgtagcc gggatgcgcg
     1621 aggatcgact tgcgcaactg ctcgcgggcc gcttcgtagt tgccctgcgc ggcgtacagc
     1681 acgccgagat tgttgtaggg ctccggcagc tcggggtagt cctgtgtcag cgcctcgaac
     1741 acggcgatcg cgtcctgcgg gcgcgcgagt tctcccagga tcacgccgcg ctggaaccgc
     1801 acctgcgcat cgcgcggatt gcgcgcgaga aacgcatcgg cgcgcgagag ggccggctcc
     1861 cattgctgct tgcccatcag cgcggcgatc tcggctgccg cgctttgcgg caccggcacg
     1921 gtcgacggcg gctcgctgac cacctgcgcc ggtgcgcctg gcgcgagcgc aagggcggcg
     1981 acaaccagca gcaagctgac aatgaaagga ggcaagtgga gcgcgcgacg ggatatactg
     2041 cttggccatt ctatcaacgc aatccttttc gacctctcga tgcacacgcg gcgctgttga
     2101 cgtccagtcg tttccttcgc gccgctgcat cgcgccgtcc gtccgacgcg cgctcgaacc
     2161 cctgcggatt gccgaccgat gacactgacc atctacaaca cgctcacgcg ccagcaggcg
     2221 ccgctcacgc cgctcgagcc gggccgggtc cggctctacg tctgcgggat gacgatctac
     2281 gacttctgtc atctgggcca cgcgcggctg atgatgacgt tcgacgtggt tgtccgctgg
     2341 ctgcggcagc gcggctacga ggtgacctac gttcgcaaca tcaccgacat cgacgacaag
     2401 atcatcaagc gcgcggccga gcgcggcatc accaccaccg cgctgacgga agagttcgcg
     2461 cgctacatgc acgaggactt cgatgcgctc ggcatcctgc ggccggacgc ggagccgcgt
     2521 gccacgcagt tcgtgccgca gatgctcggc ctgatcgaac tgctcgagaa gaagggcctc
     2581 gcgtaccagg acgatcccga cggtgacgtg aacttcgccg tacgccgctt tgccggctac
     2641 ggcaagctgt ccggcaagtc gctcgacgac ctgcgtgccg gcgagcgcgt cgcggtgggc
     2701 gagcgcaagc gcgatccgct cgacttcgtg ctgtggaagc acgccaaggc cggcgagccg
     2761 cagtgggcgt cgaagtgggg cccgggtcgc ccgggctggc acatcgaatg ttccgccatg
     2821 gcaagcgagg tgctcggacg cacgttcgac atccacggcg gcgggccgga cctgatcttc
     2881 ccgcaccacg agaacgagat cgcgcagagc gaaggcgcgt acgaacaggc gttcgccaac
     2941 gtgtggatgc attgcggcgc gttgcgcgtg ggcgaggaca agatgtccaa atcgctcggc
     3001 aatttcttca cgatccgcga tgtgctcagg cagtacggcg agaagtgggg gacggcgcgc
     3061 gccgccgagg tggtgcgctt cttcctcgtc cgcggtcact atcggagcca gatcgcctac
     3121 gacgagtcgc tgctcgcgga tgccaggagc gcactcgacc gcctgtacat cgccctgcgc
     3181 gacacgccgc cggcggccgg ggcgatcgat tggaacgagt cgcacgcgca gcggttcgcg
     3241 gcggcaatgg acgacgactt caacaccgcg ctcgccgtct ccgtgctgtt cgatctcgcg
     3301 aacgaggtca atcgcgggag gtcgccagag atggcccgcc aactgaaagc gctcggtgcc
     3361 acgctcggat tgctgcagag cgagccgcag aagtatctgc aaggtcgaga tgtcgctgtg
     3421 cacttggaag gctcggcggc gcaggtcgaa gccggtcgaa tcgaagcatt gatagcgcaa
     3481 cgtgcggctg ccaagaaaga caagaacttc gccgaagccg accgcatccg cgccgaactc
     3541 gcagtgcagg gcatcgcgct cgaggaccga ccgggcggca agaccttgtg gcggcggcaa
     3601 tagggtcgat gcagccgccg tactgggccc aggccaagcg cgcgctggcg aaatccgatc
     3661 cggtgctggc gcgcatcatt cggcgccatc cgcgcgtggc gctggcatcg cgcggcgatg
     3721 cgttctccac gctggcgcgc tccatcgtcg gacaacagat ctcggtcaag gcggccgatg
     3781 cggtgtggcg gcggctgctg ggcgcctgtc ccgaattgac gccgcccgcg gtgctgcgca
     3841 agcgcgcggc tacgctgcgc aactgcgggc tgtcggagcg caaggtcgaa tacctgcgtg
     3901 accttgcggt gcatttcgca aaagggcgcg tcgatgcgac ggcgatgcag gcgatgagcg
     3961 acgaggcggt gatcgaatgc ctgaccgaca tccgcggcat cggccgctgg acagcggaga
     4021 tgttcttgat cttcaacctg ttacggccta atgtcctgcc gctcgacgac ctcggcctgc
     4081 tgcgcgccat cagcctgaac tacctcgacg agatgccgac cgaaaacctg ctgagacgcg
     4141 aaggccgtgc gcacgtcgag cgactggcag cgccgtgggc gccgtggcgc agcgttgcca
     4201 cgtggtatct gtggcgcagc ctcgacccgg tcccggtgga gtactgatgg cgaagacaac
     4261 tttcctcgag ttcgaacagc cgatcgcgga gctggaagcg aagatcgagg aactgcgctt
     4321 cgtgcaggac gactccgcgg tcgacatttc ggaagagatc gagcggctgc agaagaagag
     4381 ccaggggctg atcaaggaca tctacgccaa gctcacgccg tggcaggtcg cgcagctcgc
     4441 gcggcatccg cagcggccgt acacgctgga ctacgtcaac gacatcttca cggacttcca
     4501 cgagctgcac ggcgatcgtg cattcgcaga cgacatgtcg atcgtcggcg ggctggcgcg
     4561 cttcaatggc cacagcgtga tggtgctggg ccaccagaag ggccgcgaca ccaaggagcg
     4621 cacgctgcgc aacttcggca tgagcaagcc ggagggctac cgcaaggcgc tgcggctgat
     4681 gaagctggcg gagaagttcg gcgttcctat cttcaccttc gtcgacacgc ccggcgcgta
     4741 tcccggcgtc gacgccgagg aacgcggcca gtcggaggcg atcggccaca acctgttcga
     4801 gatgtcgcac ctcgaagtgc cgatcgtcgc gaccatcatc ggcgagggcg gctccggcgg
     4861 cgcgctggcg atcgcggtcg ccgaccacgt gctgatgctg cagtacgcga cgtactcggt
     4921 gatctcgccc gaaggctgcg catcgatcct gtggaagagc gcggagaagg cgcccgaggc
     4981 ggccgaagcg ctcggactga ccgcgcaccg tctgaaggcg ctcggcgtga tcgacaagat
     5041 cgtctcggag ccgctcggtg gcgcgcatcg cgatccgaag cagatggccg cgctgctgaa
     5101 gcgcgcgctc ggcgacagcc tgcggcagtt ccagggcatg aagaccaagg atcttctcgc
//
LOCUS       DBNHBY010000223        17741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1366084, whole genome shotgun sequence.
ACCESSION   DBNHBY010000223 DBNHBY010000000
VERSION     DBNHBY010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 17741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1366084"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16HB_05470"
     CDS             complement(<1..469)
                     /locus_tag="AC16HB_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP-binding domain-containing protein,
                     MshE-related"
                     /protein_id="HIR5606275.1"
                     /translation="MQRIRQRGEFPVVMAEQGSNVIEQPAGADAPQAVRLGQALVSEG
                     KLSPRDLEHALAAQRETGGLIGRVLIRLGLVSELDVARALSSQLNVPLLLAEAFPPVP
                     PLVDRVAPEFFRSHRILPLDIRDDGLHVVMATPQDQFALRALRLAAGLQIVRYI"
     gene            1012..1785
                     /locus_tag="AC16HB_05475"
     CDS             1012..1785
                     /locus_tag="AC16HB_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5606276.1"
                     /translation="MTLSVISHEQHSLVDALLSDLAGIDTPELARIVLTQNLPERPPA
                     SGGSNLPPIDVIRNRSPKGFGMNHNAAFRNCDTEFFAIANPDIAMMHNPFPALISALR
                     GGAGVAAPAVLEPDGRMADTARSLITPFELIYRRTVLYSPPEHPVWCAGMFLVFRADA
                     FAAVGGFDEGYFMYCEDFDICARMRLAGWPIAYARQVQVVHAAQQLSHRSLRHFRWHL
                     SSLMRMWTSNPFWRYRSSLAAQRETAPAADSESGQEVRP"
     gene            complement(1767..3179)
                     /locus_tag="AC16HB_05480"
     CDS             complement(1767..3179)
                     /locus_tag="AC16HB_05480"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR5606277.1"
                     /translation="MLVPVILCGGAGTRLWPASRASMPKPFIKIRDEKSLLRLTHERV
                     SALADVATVVAVTNVEYGLRTAEELRGIGAELQLLLEPFGRNTAPAVAVAALWVERTY
                     GPDATLLVVPADHVVEPQADFAAAVDAARGQAESGHLVVFGVRPTRPETGYGYVKQGA
                     VLGASAFVVERFVEKPPRTEAERMLADGCYAWNSGMFCFSAAAIRTALSRYAPDVLKG
                     AEDAVASAVARAGMIEFLASAFSALPDISIDYAVMERATNVAMIPCRFSWSDIGSWKS
                     LADALPTDAHGNYSEGRALLVDSQNTYVRSGGRLVAAVGLNDLVVVETPDAVLVAHKD
                     ASQQVKDVVARLKLDGDPVATEHLTVERPWGSFTVLASTDSFKLKRIEVKPRRRLSLQ
                     MHQRRSEHWVVIEGTARVTVGDHQFDVPTGESTYVPVRTRHRLENASDGPLVIVEVQC
                     GDYVGEDDIVRFADDYGRTS"
     gene            3354..3941
                     /locus_tag="AC16HB_05485"
     CDS             3354..3941
                     /locus_tag="AC16HB_05485"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR5606278.1"
                     /translation="MNTTSLFDRAFAEHRRVFDAIAALAPAVDACAQRAAECIARGGK
                     IMFCGNGGSAGDAQHLAAELVGRLVRDRRALPGLALSTDSSALTCIANDFGYDEVFAR
                     QVEGLGRQGDVLVAISTSGNSPNVLRAAETAKRIGVYVIGFLGRDGGKLGALVDLPVT
                     VGSEETARIQEAHIFLGHVMCARIEQRLGVGGWDA"
     gene            3929..4879
                     /gene="rfaE1"
                     /locus_tag="AC16HB_05490"
     CDS             3929..4879
                     /gene="rfaE1"
                     /locus_tag="AC16HB_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR5606279.1"
                     /translation="MGRVSEISRERLARCRVLVVGDAMLDRYWFGEVERISPEAPVPV
                     IKVGATEERLGGAANVARNIVALGAQAGLLAVLGDDDAGRAIERLVRSSAIRPHLHTD
                     PALATTVKLRVIGRRQQVVRIDFESVPEAEVLAAHLDEYAALLPDYDAVLLSDYGKGG
                     LTHIARMIELARAGARPVLVDPKGDDYSRYAGATIVTPNRAELREVVGRWKSEADLAV
                     RADRLRRELGLDALLLTRSKEGMTLYTEAPPWSVPARAREVFDVSGAGDTVIGVLAVM
                     LAAGVDLRTAVEIANRAGGIVVGKLGTATVSHDELFAGRA"
     gene            4876..5868
                     /gene="rfaD"
                     /locus_tag="AC16HB_05495"
     CDS             4876..5868
                     /gene="rfaD"
                     /locus_tag="AC16HB_05495"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HIR5606280.1"
                     /translation="MRIIVTGAAGFIGANLVRALNERGETDIVAVDNLTHAEKFRNLT
                     DCEIADYLDKREFRELVQRGAAPRPDVVFHQGACSDTMESDGRYMLDNNYRYSLELLH
                     WCQEARVPLIYASSGSVYGLGPGFSEERAHETPLNVYGYSKFLFDQIVRRLLPTASAP
                     VVGLRYFNVYGPRESHKGRMASVAFHHFNQFRTGGKVRLFEGSHGYGNGEQRRDFIHV
                     DDAVRANLLFWDRPASGIYNVGTGRAQPFNDVALAVVNSLREAAGEAPLTLAHAVEQG
                     LIEYVPFPDELRGKYQAYTQADVARLRAAGLREPMMSVEHGTAAYVRWLLKNAG"
     gene            5844..6455
                     /gene="gmhB"
                     /locus_tag="AC16HB_05500"
     CDS             5844..6455
                     /gene="gmhB"
                     /locus_tag="AC16HB_05500"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR5606281.1"
                     /translation="MAAEERGMSARRRAAFLDRDGVINNDHGYVARIEDFEFVPGTFD
                     ACSALARHGFALVVITNQSGIGRGLYSEADYHTLTAWMRAQFAAAGAPLAGVYHCPHH
                     PTDAYGIFRIECDCRKPAPGMLLRAAAELTLDLERSIVFGDRRSDLQAAHAAGVPHRI
                     LLGTDGVAQPDTDDGGFATARFRSLAEAVSSVELHALMAAEHV"
     gene            6448..6945
                     /locus_tag="AC16HB_05505"
     CDS             6448..6945
                     /locus_tag="AC16HB_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR5606282.1"
                     /translation="MSEPKLPPPDFVRKIVSRDQLATAVARLPRPIVFTNGVFDILHR
                     GHVTYLAQARALGASLVVAVNSDASVRLLGKGDDRPINSQDDRAAVLAALESVSLVIV
                     FDEQVPMAVLDAVRPEVYVKGGDYDMSRVPEAQRAQSWGALAVAIPILFQRSTTSLLH
                     RLRGR"
     gene            7111..8172
                     /locus_tag="AC16HB_05510"
     CDS             7111..8172
                     /locus_tag="AC16HB_05510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5606283.1"
                     /translation="MPLLLQASLACLLSALMVVALRVPAARLRLIDHPDGRKRHGEAV
                     PVTGGLAILLAFFGALAIPAGTAEMHRVLTVAMLGLGAVGLYDDSHGGSARAKLVAQI
                     AAALVMTLWGNQLLLSLGDILAIGPIGLKAWSIPVTVFATVAVVNGINMLDGLDGLAG
                     GTVAVMLVYFALFAWLLGDARALTLLAVLLGAVVGFLVFNAPHPWRGRLRTFMGDTGS
                     LALGFVVAWFTLELSQKAHAVPPVVMLWVAGVVLLDLFTVTLRRLLRGRNPLVGDRGH
                     IHHLLLRRGASPAVAAATLVGANALLGAIGTAGWLIGIAEAWLFAGFLAVGVAYLAVF
                     LNPARLMRGTGRRRPSKRR"
     gene            8181..8996
                     /locus_tag="AC16HB_05515"
     CDS             8181..8996
                     /locus_tag="AC16HB_05515"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR5606284.1"
                     /translation="MASIVAIGDVQGCVGRLRQLLGAHDGNERLVFVGDIVNRGPESL
                     DSLRFAKSLGNRATVLLGNHDLHLLAVAAGIRPQHGDDTLQEVLDAPDRDELLDWLRA
                     RPLAHCEAGALFVHAGVLPQWSVEKTMQLAGEVQARLRADDYRGFLATMYGNRPARWD
                     DALVGADRARCVINALTRLRFVDADGTMDFKVKEGIDAAPPGFMPWFDHPQRATRTTP
                     VIFGHWSALGLLLRGDAICLDTGCVWGGRLSALRWPDRSLIQVDCPQYRAPKG"
     gene            complement(8923..9786)
                     /locus_tag="AC16HB_05520"
     CDS             complement(8923..9786)
                     /locus_tag="AC16HB_05520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5606285.1"
                     /translation="MRALIAPLRIVALLVHILVGLAIAVAIFPWQSQRRRNPTIRAWS
                     RALLAICGMRLRVTGITLDPELARSGALDWTPGRLLLANHVSWIDVFAILATLPSRFV
                     AKSEIRRWPLLGWLVTLVGTLYVERGRRHAVAAINHRVRDRLKAGESVAVFPEGTTTD
                     GASMLPFHSNLIAPALEIGCDVWPVALRYTERGGPSSAPAFVGEMGLMTSLWNILIAR
                     DLAVEVAVLSPVGTSSEGNRHRVAHVAQDAIAARLGLMAPQPRRGVNPSAPGTAGNRP
                     ESATDPASAAR"
     gene            complement(9806..11077)
                     /locus_tag="AC16HB_05525"
     CDS             complement(9806..11077)
                     /locus_tag="AC16HB_05525"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5606286.1"
                     /translation="MKLALRNGRLIDPASGHDAVADLFIADGRVVAVGRAEDFAADRT
                     IDARGLIVAPGLVDLCARMREPGAEGALKVEMRAALAGGVTGVVCPPDTDPPLDEPGL
                     VRMLRQRSREAAGARLYPLGALTAGLAGEALAEIETLHEVGCVAFMQTRALPHDNSVL
                     LQAMRYAKTFDLPLWLRPVEDTLAGSGVAAAGAVAGRLGLPSIPVAAETVALHTVFEL
                     QRAAGNPRVHLARLSSGAGVELVRQAKREGLPVTADVGIHHVHLIDVDIGYFDPNFRL
                     DPPLGSQRDRDAILAGLADGTIDAICSDHTPVGADDKLLPFGEATPGATALETLLPLV
                     LKWASRADVPLVRALGKVTSDPARVLGEPTGRLAVGAKADVVVFDPIAHWRVLPEALV
                     SDGKNTPFTGHELEGRARFTLVGGEVRFERR"
     gene            complement(11074..12033)
                     /locus_tag="AC16HB_05530"
     CDS             complement(11074..12033)
                     /locus_tag="AC16HB_05530"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR5606287.1"
                     /translation="MRNPQLNANGELHHLLSLDGLPRAIIERILTTADSFINVAAREV
                     KNVPLLRGKAVFNLFFENSTRTRTTFEIAARRLSADVFNLDIMRSSTAKGESLLDTIN
                     NLTAMQADMFVVRHAASGAPFLIAQHVAPHIHVINAGDGRHAHPTQGLLDMYTIRHYK
                     RDFTNLTVAIVGDIQHSRVARSDIHALTTLGVPEVRAVGPLTLLPAGLPEMGVRVCTR
                     LDEGIRDADVIIMLRLQNERMRGALLPSAQEFFRNYGLTPEKLRLAKPDAIVMHPGPM
                     NRGVEIDSHVADGVQSVILPQVTFGIAVRMAVLSIASSSQRMQ"
     gene            complement(12026..12529)
                     /gene="pyrR"
                     /locus_tag="AC16HB_05535"
     CDS             complement(12026..12529)
                     /gene="pyrR"
                     /locus_tag="AC16HB_05535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR5606288.1"
                     /translation="MISLDAEAIYRSFAQRVREEVAGRDVRLVGVHTGGVWLAERLKE
                     ELRNPHPVGVLDISFYRDDFHRAGLNPSVKPSRIPFDIEGADVLLIDDVLYTGRTVRA
                     AMNELFDYGRPARIELAVLIDRGGRELPIAAIVVGRTIEVPADVSVVLARDDSGRLGL
                     TLEQKRA"
     gene            complement(12526..12900)
                     /gene="ruvX"
                     /locus_tag="AC16HB_05540"
     CDS             complement(12526..12900)
                     /gene="ruvX"
                     /locus_tag="AC16HB_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5606289.1"
                     /translation="MTDAVVLGFDFGARRIGVAIGNSVTREARPLAVIDAQGAARWTR
                     IGALIEEWQPSQFVVGIPRHPDGTPHEMTARCERFARQLAGRWQRAVARVDERYSSAV
                     VRDGADDEAAAVILQQWLEERR"
     gene            complement(12953..13519)
                     /locus_tag="AC16HB_05545"
     CDS             complement(12953..13519)
                     /locus_tag="AC16HB_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR5606290.1"
                     /translation="MAKAQMNLTHQFLIAMPTLVDPNFERTVVYVCEHNDKGAIGLVI
                     NRSTDLTLAKLFDKIDLKLEIAPWKDAPVYFGGPVQTERGFVLHSPAGNYSSSLTVAD
                     EIALTTSKDVLEAVASGAGPDKLLVTLGHSGWGGGQLENEIAHNAWLTVAARAEIIFE
                     TPVEQRFDAALKLLGVDSAQLSGLAGHA"
     gene            13589..15028
                     /locus_tag="AC16HB_05550"
     CDS             13589..15028
                     /locus_tag="AC16HB_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR5606291.1"
                     /translation="MDAALVWFRRDLRLRDHAALYHALRAARRVYCGFVFDRAILDPL
                     LAEGIVADRRVEFIHRAVTELAQALDERGGALLVRHGFASEEIVRLAAELHVDAVFAN
                     HDYEPDAIGRDRHVDAELAATGRRLLTFKDQVIFEKEEVVTVAGRPFSVFTPYRVAWL
                     KKLTPFYVKPYPVERHATALARPPRVEPLPTLAELGFAGTNLGRLRIAAGAAGGAQAF
                     TEFKARIDDYDRLRDFPSRNGPSYLSVHLRFGTVSIRELADFASERAAAESRGAAIWL
                     SELIWRDFFQQILFHHPHVVSGAFRPEYDRIRWRDDPELLAAWCEGRTGYPLVDAAMA
                     QINRTGYMHNRLRMVVASFLVKDLGIDWRRGERYFARHLNDFDLAANNGGWQWAASSG
                     CDAQPYFRIFNPVAQSEKFDPDGAFIRRYLPQLAGFSPREIHAPWRVPIARQRDAGCV
                     IGVDYPAPIVDHAQARAQTLARYAAVRRA"
     gene            complement(15015..16178)
                     /locus_tag="AC16HB_05555"
     CDS             complement(15015..16178)
                     /locus_tag="AC16HB_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606292.1"
                     /translation="MLIALGVTQIVSWGSIYYGFALLLEPLQHDLGAGKDAVAGAFSV
                     ALLASGLCSTRIGRTIDRLGGRGVMTLGSLAAAALLAALSRVETLAALYVVWAGLGVA
                     MAATLYEPAFAVITQIFALGYRRALTVLTLFGGFASTVFWPLTTWLIERHGWREATLW
                     LALINLLVCVPLHYWLLPRARTGRAAESAPFSKAAHPPWHERRFRALALAFLAHYVVV
                     SAVAAHLIALLLARGLSPTAAAGIGALIGPMQVAGRVVEFGASRWVHAGRVGRIAASL
                     LPASLLALLVADGGAALLVLFAVLYGAGNGAMTVVRGALPAELYGREQYGAIAGALAM
                     PGLLARAVGPILAALLWTVLGGYDRVTMVLIAVASLGAVAFYYATVEREIRRA"
     gene            complement(16312..>17741)
                     /gene="groL"
                     /locus_tag="AC16HB_05560"
     CDS             complement(16312..>17741)
                     /gene="groL"
                     /locus_tag="AC16HB_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753915.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5606293.1"
                     /translation="VASRTSDDAGDGTTTATVLAQSIVAEGMKYVAAGMNPMDLKRGI
                     DKAVTAAVEELKKISKPCTTNKEIAQVGAISANADENIGDIIAKAMDKVGKEGVITVE
                     DGKSLDNELEIVEGMQFDRGYLSPYFINNADKQIAILDNPYVLLHDKKVSNIRDLLPI
                     LEQVAKAGRPLLIVAEEVEGEALATLVVNNIRGILKTCAVKAPGFGDRRKAMLEDIAI
                     LTGGNVIAEETGMTLEKATLNDLGQAKRIEIAKENTTIIDGAGDAKAIEARVKAIRAQ
                     IEEATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALHATRAAVEEG
                     IVAGGGVALIRAKQAISKVKGDNTDQEAGIKIVLRAMEEPLRMIVENSGYEPSVVVNK
                     VAEGKGNFGFNAQTGEYGDLVTMGVLDPTKVTRTALQNAASVAGLMLTTDAMVAELVE
                     EKPAGGGGGMPGGMGGMGGMGGMDM"
BASE COUNT         2824 a         6086 c         5865 g         2966 t
ORIGIN      
        1 cgatataccg gacgatctgc aggccggccg ccagacgcag tgcgcgtagc gcaaactggt
       61 cctgcggcgt cgccatcacg acgtgcagcc cgtcgtcgcg gatatcgagc ggcagaatcc
      121 gatgcgagcg gaagaactcg ggcgccacac gatcaaccag cggcggcaca ggtggaaacg
      181 cctccgcgag cagaagcggc acattcagct ggctcgacag cgcccgtgcg acatccagtt
      241 cggataccag cccaagccgg atcagcaccc gcccgatcag cccgccggtt tcgcgctgcg
      301 cagccagcgc gtgctccagg tcacgcggcg agagcttgcc ctcggacacg agcgcctggc
      361 ccagacgaac cgcctgcggt gcatccgcgc ccgcaggctg ctcgatcacg ttcgaacctt
      421 gttctgccat gaccaccggg aattcgccgc gctgtcggat ccgctgcaag tcacaacctt
      481 gtacacgcga tctgcgccgc aagtgtttcg tccggtaacg gatcggcgac cggagcccgg
      541 cggaattttg tcatgaatga ccacgcacaa cgctgcggcc gttcgagacg gcgcggtgct
      601 cggcatcgat ggatcggccg ggcgctgtcg cgtcgtccat gggcgcgtgc ggcccgacgt
      661 gctgcatccc gaagcgaccg cgatgccgga aaactcttgt tacgcgatcg cctgcaggat
      721 cctcggcgcc gtgtctattc ttcgaacatc cggatcgtgc gggtggatgc gtttcgaccg
      781 ccaagatcaa gcttgcacga ccctgtacca aggataggtg gccacccgat cggccacacg
      841 cgcgttcacc tgaaccggac cccaacctgc ggatcgaccc atgcgagcgg acgagtatta
      901 tcgcggccca tgatgcgatg catgagcccc gcattgccgc tgctgcggct ccgctagaac
      961 aacaaccctc actcgcgacg cttcttccat tccagaatga ataaagcatg cgtgaccttg
     1021 tcggtcatca gccatgagca gcattcgctc gtcgatgcgc ttctgtccga tctggcgggt
     1081 atcgacaccc ccgaactggc gcgaatcgtt ctgactcaga acctgccgga gcgccctccg
     1141 gccagcggcg ggtcaaatct gccgccgatc gatgtgattc gcaatcgttc gccgaagggg
     1201 ttcggtatga accataacgc ggcgttccgc aattgcgaca cggagttctt cgcgatcgcc
     1261 aaccccgaca tcgcgatgat gcacaacccg ttcccggcgc tcatttccgc gctgcgtggc
     1321 ggcgccggcg tcgcggcgcc ggcagtgctg gagcccgatg gtcgcatggc cgacaccgcg
     1381 cgcagcctga tcactccgtt cgaactgatc taccgtcgca ccgttttgta ttcgccgcca
     1441 gaacacccgg tctggtgtgc cggcatgttc ctcgtgttcc gcgcggacgc gtttgccgcc
     1501 gtcggcggat tcgacgaggg ctacttcatg tactgcgagg acttcgacat ctgcgcgcgc
     1561 atgcgactgg ccggctggcc gattgcctat gcgcgtcagg tccaggtcgt gcacgccgcg
     1621 cagcagttga gccatcgctc gctgcgccac tttcggtggc acttgtcgag cttgatgcgg
     1681 atgtggacgt cgaatccgtt ctggcgctac cggtcttcac tcgcggcgca gagggaaacc
     1741 gcgccggcag cggattcgga atcaggtcag gaagtccgcc cgtagtcgtc ggcgaagcgg
     1801 acgatgtcgt cttcgccgac atagtcgccg cactgcacct cgacgatcac cagcggcccg
     1861 tcgctcgcat tctccagccg gtgccgcgtc cgtaccggca catacgtcga ttcgccggtc
     1921 ggcacatcga actgatggtc gcccacggtg acgcgcgcgg tgccctcgat cacgacccag
     1981 tgctcgctgc ggcgctgatg catctgcagc gacagccggc gtctgggctt gacctcgatg
     2041 cgcttgagct tgaaggaatc cgtgctggcc aggacggtga aacttcccca cggacgctcg
     2101 acggtcaagt gctcggtggc gaccggatcg ccgtcgagtt tcagtctggc gacgacgtcc
     2161 ttgacctgct ggctggcatc cttgtgagcc accagcaccg cgtccggggt ttcgaccacc
     2221 accaggtcgt tcagccccac cgcggcgacg agacgcccgc ccgatcgcac gtaggtgttc
     2281 tgcgaatcga ccagcaacgc cctgccctcg ctgtagttgc cgtgcgcatc ggtgggcaat
     2341 gcgtcggcta gcgacttcca ggaaccgatg tcgctccagg aaaagcggca cggaatcatc
     2401 gccacgttgg tcgctcgttc catgacggcg tagtcgatcg agatatccgg cagcgcactg
     2461 aaggccgacg cgaggaactc gatcatcccg gcgcgcgcaa cggcgctggc gacggcatcc
     2521 tccgctccct tcagaacatc cggcgcatac ctggaaagcg cggtgcgaat ggccgcggcg
     2581 ctgaagcaga acatgccgct gttccacgca tagcagccgt ccgccagcat gcgctcggcc
     2641 tccgtgcgtg gcggcttctc gacgaagcgc tccacgacga aggcactcgc acccaacacg
     2701 gccccttgct tgacgtatcc gtatccggtt tccgggcgcg tcggtcgcac gccaaacacg
     2761 acgagatggc cggattcggc ctgaccgcgc gccgcgtcga cggccgcggc gaagtcggcc
     2821 tgcggctcga ccacgtggtc agccggaacg acgagcagcg tggcgtccgg gccgtaggtc
     2881 cgctcgaccc acagcgcggc gaccgccacc gcaggagcgg tattgcggcc gaagggctcg
     2941 agcagcaatt gcagctcggc gccaatgccg cgcagttcct ccgcggtccg cagtccgtac
     3001 tcgacattcg tcaccgcgac aactgtcgcc acgtcggcga gcgcactcac gcgttcgtgc
     3061 gtaagacgca gcaggctctt ttcgtcgcgg atcttgatga aaggcttcgg catcgaggcg
     3121 cgagaagcag gccaaagccg ggttccggcg ccaccgcaga gaatgactgg aacgagcatc
     3181 gggataggaa agaggggttg gacaaatgcg ccggcgccga ttcgcgcgcg cgaacgcaac
     3241 gatgttcgca caccgccttg cccaccgagg cgacgacggc cgggcggttg gaccaatgcg
     3301 gtgagcggcg cactacctat aatccgcacc ttgccgaatc gcagcgcgca tgaatgaaca
     3361 cgacctcgct gtttgaccgg gcgttcgctg agcaccggcg cgtgttcgac gccatcgccg
     3421 cactcgcacc ggccgtggac gcctgcgcgc agcgggccgc ggaatgcatc gcacgcggcg
     3481 gcaagatcat gttctgcggc aacggcggat cggcgggaga cgcgcagcat ctggcggcag
     3541 agctggtcgg ccggctcgtg cgcgaccggc gtgcgctgcc gggactggca ttgtcgaccg
     3601 attcatcggc gctgacgtgc atcgccaacg atttcggcta tgacgaggtg ttcgcgcgcc
     3661 aggtcgaggg gctcggccgt cagggcgatg tgctggtagc gatttccacg tccggcaatt
     3721 cgcccaatgt gctgcgcgcg gcggagaccg cgaagcgcat cggcgtgtat gtgatcggct
     3781 tcctcggccg cgacggcggc aagctcggtg cgctggtcga cctgccggtc accgtcggat
     3841 cggaagagac cgcgcgcatc caggaggcgc atatcttcct gggccacgtg atgtgcgcgc
     3901 gcatcgagca gcggctcggc gtcggcggct gggacgcgtg agcgagattt cgcgcgagcg
     3961 gctggcgcgc tgccgcgtgc tcgtggtggg cgacgcgatg ctcgaccgct actggttcgg
     4021 cgaggtcgag cgaatctcgc ccgaggcacc ggtgccggtc atcaaggtcg gcgcgactga
     4081 ggaacggctc ggcggcgcgg caaacgttgc gcgcaacatt gtcgccctcg gcgcgcaggc
     4141 gggcctgctt gcggtgctcg gcgacgacga cgcggggcgt gcgatcgaac ggctcgtcag
     4201 gagcagcgcg atccgtccgc acctgcacac cgatccggcc ctggcgacga cggtcaagct
     4261 gcgggtgatc ggccggcggc agcaggtcgt gcgcatcgat ttcgagagcg ttcccgaggc
     4321 cgaggtgctg gcagcgcatc tggacgaata cgcggcgctg ctgcccgact acgacgcggt
     4381 gctgctgtcg gattacggca agggcgggct cacgcacatt gcgcgcatga tcgaactggc
     4441 gcgcgcgggc gcgcggcccg tgctggtcga cccaaagggc gacgactact cgcggtacgc
     4501 gggcgcgacg atcgtgacgc ccaaccgtgc ggaactgcgc gaagtagtcg gacgctggaa
     4561 gagcgaagcc gacctcgcgg tgcgcgcgga caggctgcgg cgcgaactcg gactcgatgc
     4621 gctgctgctg acacgcagca aggaaggcat gacgctgtac accgaggcgc cgccctggtc
     4681 ggtgccggca cgggcgcgcg aagtgttcga cgtgagcggc gccggcgaca cggtgatcgg
     4741 cgtgctggcg gtgatgctgg cggccggcgt cgacctgcgc accgcagtcg agattgcgaa
     4801 tcgcgccggc ggcatcgtgg tcggcaagct ggggacggcg accgtatcgc acgacgaact
     4861 cttcgcgggg cgcgcatgag gatcatcgtc accggcgcgg ccggattcat cggcgccaac
     4921 ctcgtccgcg cgctgaacga gcgcggcgag accgacatcg ttgcggtcga caacctgacg
     4981 catgccgaaa agttccgcaa tctgaccgac tgcgagattg ccgactacct cgacaagcgc
     5041 gagttccgcg agctcgttca gcgcggtgcc gcaccgcggc ccgacgtggt gtttcatcag
     5101 ggcgcctgct cggacacgat ggagtccgac ggccgctaca tgctcgacaa caactaccgc
     5161 tattccctcg aactgttgca ctggtgccag gaggcgcgcg tccccctcat ctatgcatcg
     5221 tcggggtcgg tctatgggct cggcccgggc ttcagcgaag agcgcgcaca cgaaacgccg
     5281 ctgaacgtct acggctactc caagttcctg ttcgaccaga tcgtgcggcg cctgctgcct
     5341 accgcgagcg cgccggtcgt cggattgcgc tacttcaatg tctacggccc gcgcgagtcg
     5401 cacaagggtc gcatggcatc ggtggcgttc catcacttca accagttccg caccggcggc
     5461 aaggttcgcc tgttcgaggg cagccatggc tacggtaacg gcgagcagcg gcgcgacttc
     5521 atccacgtcg acgacgcggt gcgggccaac ctgttgttct gggaccgacc tgccagcggc
     5581 atctacaacg tgggcaccgg gcgcgcgcag ccgttcaacg acgtggcgct cgcggtggtg
     5641 aattcgctgc gcgaagcggc cggggaggcg ccgctcacgc ttgcgcacgc cgtcgaacag
     5701 ggattgatcg aatacgtgcc tttcccggac gaattgcgcg gcaagtacca ggcctatacg
     5761 caggccgacg tggcgcgcct gcgcgcggcc gggttgcggg aaccgatgat gtcggtcgag
     5821 cacggcacgg ccgcgtacgt gcggtggctg ctgaagaacg cgggatgagc gcccggcgac
     5881 gcgcggcatt cctggatcgc gacggcgtga tcaacaacga tcacggctac gtggcgcgga
     5941 tcgaagactt cgagttcgtc cccggcacgt tcgacgcctg cagcgcgctg gcgcggcacg
     6001 gcttcgctct ggttgtgatc accaatcagt ccggcatcgg ccgcgggctg tactcggagg
     6061 ccgactatca cacactgacc gcatggatgc gcgcgcagtt cgccgccgcc ggcgcaccgt
     6121 tggcgggcgt gtaccactgc ccgcaccacc cgaccgacgc gtacgggatc tttcgcatcg
     6181 aatgtgattg ccgcaagccc gccccgggca tgctgctgcg tgcggcggcc gagctgaccc
     6241 tggacctgga gcgctcgatc gttttcggcg atcggcgctc ggatctgcag gctgcgcacg
     6301 ccgccggcgt gccgcatcgg atcctgctcg gcaccgacgg cgtcgcgcaa cccgataccg
     6361 acgacggcgg attcgcgaca gcgcgtttcc gctcgctggc cgaggcggtt tcgagcgtgg
     6421 aactgcacgc attgatggcg gccgagcatg tctgaaccga agctcccgcc gccggacttc
     6481 gtgcgcaaga tcgtgtcgcg cgatcagctc gcaaccgcgg tggcccgcct gccgcgcccg
     6541 atcgtcttca ccaacggcgt gttcgacatc ctgcaccgcg gccatgtcac gtacctcgcg
     6601 caggcgcgcg cgctgggcgc gagcctcgtc gtcgcggtga actcggacgc ttcggtgcgg
     6661 ctgctgggca agggcgacga tcggccgatc aactcccagg acgatcgtgc ggccgtgctg
     6721 gcggcgctgg aatcggtgtc attggtgatc gtgttcgacg aacaggtgcc gatggcggtg
     6781 ctcgatgccg tgcgaccgga ggtctacgtc aaagggggcg attacgacat gtcgcgggtc
     6841 cccgaagccc agcgcgcgca atcgtggggc gcactcgcag tcgcaattcc aattctgttc
     6901 cagcgctcga cgacctcgtt gttgcaccga ctgcgcggcc gctgagccct tcggcgtgac
     6961 gagttcccgc cggcggtcga actcgaatgg gctgtgcgaa aatgcccccg aggcagtgag
     7021 cgaccggtac gatcgagtcg ctcccttcgt tccccgtatt cagcgttcga tcccaccgtc
     7081 gacccgggcc cgcggccgtt tgcttcttcg atgccacttc tcctgcaagc gtcgctcgcc
     7141 tgtctgctgt ccgcgttgat ggtcgtcgcg ctgcgcgtac cggccgcacg attgcggctc
     7201 atcgaccatc cggacggtcg caagcgccat ggcgaggccg taccggtgac cggcggactg
     7261 gcgatcctgc tggcgttctt cggcgcgttg gccattccgg ccggaactgc cgagatgcat
     7321 cgcgtgttga ccgtcgcgat gctcggactg ggagcggtcg ggctctacga cgacagccac
     7381 ggcggttctg cccgcgcgaa gcttgtcgcg caaattgccg ccgcgttggt gatgacgctg
     7441 tggggcaacc agttgttgct ttcgctgggc gacatccttg ccatcggacc catcggcttg
     7501 aaggcgtggt ccattccagt caccgtgttt gcgaccgtgg ccgtggtcaa tggcatcaac
     7561 atgctcgatg ggctcgatgg tctggcgggc ggtacggttg ccgtcatgct cgtttacttc
     7621 gcgttgttcg cctggctgct cggcgatgcg cgtgcgctga cgctgctcgc ggtgctgctg
     7681 ggcgccgtcg tcggattcct ggtcttcaat gcgccgcatc cttggcgcgg ccgcctgcgc
     7741 actttcatgg gcgacaccgg cagcctcgca ctcggcttcg tcgtcgcgtg gttcacgctc
     7801 gaactgtcgc agaaggcgca cgccgtgccg ccggtggtga tgctgtgggt cgcgggcgtg
     7861 gtcctgctcg acctgttcac ggtcacgctg cgtcgcctgc tgcgcggccg caatccgctg
     7921 gtcggagatc gcggccacat ccatcacctc ctgctgcgcc ggggtgcctc cccggccgtc
     7981 gctgccgcga cgctcgtggg cgcgaatgcg ctgctcggcg cgatcggaac ggccggatgg
     8041 ctgatcggaa tcgcggaggc atggctgttc gcgggattcc tcgcagtcgg ggtcgcatat
     8101 ctcgcggtct tcctgaatcc agcacggctg atgcgcggga ccggacggcg gcgtccgtcg
     8161 aaacgtcgct agcgattccg atggcatcga ttgtggccat tggcgacgtc cagggctgcg
     8221 tcgggaggct gcgccaattg ctcggcgcgc atgatgggaa cgagcgcctg gtcttcgtcg
     8281 gcgacatcgt caaccgcggc ccggaatcgc tcgactcgct gcgcttcgcg aaatcgcttg
     8341 gcaaccgtgc gaccgtgctg ctcggcaatc acgatctgca cctgcttgcg gtcgctgcgg
     8401 gcatccggcc gcagcacggc gacgacacgc tgcaggaagt cctcgacgca cccgaccgcg
     8461 atgaactgct cgactggctg cgcgcccgcc cgctggcgca ctgcgaggcc ggcgccctgt
     8521 tcgtgcacgc gggcgtgctg ccgcagtggt ccgtcgagaa gaccatgcag ctcgctggcg
     8581 aggtgcaggc caggctgcgt gccgacgact accgcggatt cctggcgacg atgtacggca
     8641 accggccggc acgctgggac gatgcattgg tcggcgccga tcgtgcgcgc tgcgtgatca
     8701 acgcgctcac gcgactgcgt ttcgtcgacg cagacggcac gatggacttc aaggtcaagg
     8761 aaggcatcga tgccgcacca ccgggattca tgccgtggtt cgatcatcca cagcgggcca
     8821 cgcgcaccac tccagtgatc ttcggccact ggtccgccct cggcctgctg ctgcgcggtg
     8881 acgcgatctg cctggacacc ggctgcgtgt ggggcggccg gctcagcgcg ctgcgctggc
     8941 cggatcggtc gctgattcag gtcgattgcc cgcagtaccg ggcgccgaag ggttgacgcc
     9001 gcgccgcggc tgcggagcca tcaggccgag acgcgcggcg atcgcgtcct gcgcgacgtg
     9061 cgcgacgcga tggcgattgc cctccgagct cgttccgacc ggcgacagca cggcgacctc
     9121 gaccgccaga tcgcgcgcga tcagaatgtt ccatagcgac gtcatcagtc ccatctcgcc
     9181 gacgaacgcc ggcgcgctcg agggcccacc gcgctcggtg tagcgcagcg cgaccggcca
     9241 cacgtcgcac ccgatctcga gcgcgggcgc gatcaggttc gaatgaaacg gcagcatcga
     9301 ggcgccgtcg gtggtcgtgc cttcgggaaa caccgcgacg gattcgccgg ccttcagacg
     9361 atcgcgcacg cgatggttga tcgccgccac cgcgtggcgc cggccgcgct cgacgtacag
     9421 cgtgcccacc agcgtgacca gccagcccag cagcggccag cgccggatct cggacttggc
     9481 cacgaagcgg ctgggcagcg ttgcgagaat cgcgaacacg tcgatccacg agacgtgatt
     9541 ggccagcagc agccttcccg gtgtccagtc aagcgcgccg ctgcgcgcga gttcgggatc
     9601 gagcgtgatg ccggtgacgc gcagacgcat accgcagatc gccaggagcg cccgtgacca
     9661 ggcgcggatc gtgggattgc gccgccgctg cgactgccac ggaaagatcg cgaccgcgat
     9721 cgccagtccg accaggatgt gaacgagcag cgcaacgatc cgcagcggtg cgatcagcgc
     9781 acgcattgcg gtcgcagcct gcgccctaac ggcgctcgaa acggacttcg ccgcccacca
     9841 gggtgaaacg ggcccggccc tcgagttcat ggccggtgaa cggggtgttc ttgccgtcgc
     9901 tcacaagcgc ctcgggcaga acgcgccagt gcgcgatcgg gtcgaacacg accacgtcgg
     9961 ccttcgcgcc gaccgcgagt ctcccggtcg gttcgccgag cactcgcgcc ggatcgctgg
    10021 tgaccttgcc gagcgcgcgc acgagcggca cgtcggcgcg gctggcccac ttgagcacga
    10081 gcggcagcaa cgtctcgagc gcggtggcac ccggcgtcgc ttccccgaac ggcagcagct
    10141 tgtcgtcggc gccgaccggc gtgtggtcgg agcagatcgc atcaatcgtg ccgtcggcga
    10201 gccccgccag aatcgcgtcg cgatcgcgct gtgatcccag cggcgggtcg agccggaaat
    10261 tcggatcaaa gtagccgatg tcgacatcga tcaggtgcac gtgatggatg ccgacatcgg
    10321 ccgtcacggg cagaccttcg cgcttggcct ggcgcacgag ttcgacgccg gcgcctgacg
    10381 acagccgcgc cagatgcacg cgcggattgc ccgccgcgcg ctgcagttcg aacacggtgt
    10441 gcagcgcgac cgtctcggcc gcaaccggaa tcgacggcag cccgagtcgc cccgccactg
    10501 cgcccgcagc ggccacgccg ctgccggcaa gcgtgtcctc gaccggccgc agccacagcg
    10561 gcaggtcgaa cgtcttcgcg taacgcatcg cctgcaacag gacgctgttg tcgtgcggca
    10621 gtgcgcgcgt ctgcatgaac gccacacagc ccacctcgtg cagcgtctcg atctcggcca
    10681 gagcctcacc tgccagcccc gccgtcagtg cgccgagcgg atacagtcgc gcaccggccg
    10741 cttcgcgcga acgctggcgc agcatgcgca ccaagcccgg ctcgtcgagc ggcggatcgg
    10801 tatcgggcgg gcacaccacg cccgtcacac cgccggcgag tgccgcgcgc atttccacct
    10861 tcagcgcgcc ttcggcaccg ggctcgcgca tgcgcgcgca gaggtcgacg agtcccggcg
    10921 caacgatcag gccgcgcgca tcgatcgtgc gatccgccgc gaaatcttcc gcgcggccga
    10981 ccgcgacgac acggccgtcg gcgatgaaca gatcggcgac cgcgtcgtga cccgacgcgg
    11041 gatcgatcag gcggccgttc ctgagcgcga gcttcactgc atcctctgcg acgacgaggc
    11101 gatcgacagc accgccatcc gcaccgcgat gccgaacgtg acctgcggca ggatcacgct
    11161 ctgcacgccg tcggcgacgt gcgagtcgat ttccacacca cggttcatcg ggcccgggtg
    11221 catcacgatc gcgtcgggct tggcgagcct gagtttctcc ggcgtcagcc cgtagttgcg
    11281 gaagaattcc tgcgcgctcg gcagcagcgc cccgcgcatc cgctcgttct gcagccgcag
    11341 catgatgatg acatcggcat ccctgatgcc ctcgtccagc cgcgtgcaga cgcgcacgcc
    11401 catttcgggc agtcccgccg gcagcagcgt cagcggcccg actgcgcgca cctcgggcac
    11461 accgagcgtg gtcagcgcgt ggatgtcgga ccgcgccacg cgcgagtgct ggatgtcgcc
    11521 gacgatcgcg accgtcaggt tcgtgaagtc gcgcttgtaa tgccggatcg tgtacatgtc
    11581 gagcaggccc tgcgtcgggt gcgcatggcg gccgtcgccg gcattgatca catgaatgtg
    11641 gggcgccacg tgctgcgcga tcaggaacgg cgcaccgctg gcggcatggc ggaccacgaa
    11701 catgtcggcc tgcatcgcgg tcaggttgtt gatcgtgtcc agcagcgact cacccttggc
    11761 ggtggacgag cgcatgatgt cgaggttgaa cacgtcggcg gacagccgcc gcgcagcgat
    11821 ctcgaacgtg gtgcgcgtgc gcgtgctgtt ctcgaagaac aggttgaaga ccgccttgcc
    11881 gcgcagcagc ggaacgttct tcacctcgcg cgcggcgacg ttgatgaagc tgtcggccgt
    11941 ggtcaggatg cgctcgatga tcgcgcgcgg cagcccgtcg agcgaaagca ggtgatgcag
    12001 ttcaccgttc gcattgagct gcggattacg cacgcttctg ctccagcgtc agtccgaggc
    12061 gacccgagtc atcgcgcgcc agcacgacgc tgacgtcggc gggcacctcg atcgtgcgac
    12121 cgacgacgat cgcggcgatc ggcagctcgc gcccgccgcg atcgatcagc accgcgagtt
    12181 cgatccgcgc tggacggcca tagtcgaaca gttcgttcat cgccgcgcgt accgtgcggc
    12241 cggtgtacag cacgtcgtcg atcagcagga cgtcggcgcc ttcgatatcg aacggaatgc
    12301 gcgagggctt cacgctcggg ttcagccccg cacggtggaa gtcgtcgcgg tagaacgaga
    12361 tgtcgagcac gccgaccgga tgcggattcc ggagctcttc cttcagccgc tccgccagcc
    12421 acacgccgcc ggtatgcacg ccgaccagcc gcacgtcgcg tccggcgacc tcctcgcgca
    12481 cgcgctgcgc gaagctgcgg tagatcgctt cggcgtcgag cgaaatcatc ggcgctcttc
    12541 cagccactgc tgcaggatca cggcggccgc ctcgtcatcg gcgccgtcgc gcacgacggc
    12601 cgatgaatag cgctcgtcga cgcgcgcgac cgcacgctgc cagcgccccg cgagctgacg
    12661 ggcaaagcgc tcgcagcgcg cggtcatctc gtgcggcgtg ccgtcgggat ggcgcggaat
    12721 gccgaccacg aactgcgacg gctgccattc ctcgatcagg gctccgatcc gcgtccagcg
    12781 cgcggcaccc tgtgcgtcga tcaccgcgag cggccgcgcc tcgcgcgtga ccgagttgcc
    12841 gatcgccacg ccgatgcggc gtgcgccgaa gtcgaaaccg agcacgacgg catcggtcat
    12901 cggggagaga agagcggagt gcggccggcc cgaaggtgtg aggcacacgc ggtcaagcat
    12961 gcccggccag ccccgacagt tgcgccgagt cgacaccgag cagcttcagc gccgcatcga
    13021 agcgttgctc gaccggcgtc tcgaagatga tctccgcgcg cgcggccacc gtcagccatg
    13081 cgttgtgcgc gatctcgttc tccagctgtc cgccgcccca gcccgagtgg ccgagcgtca
    13141 ccagcagttt gtccgggccc gcgccactgg ccaccgcctc gagcacgtcc ttcgacgttg
    13201 tcagcgcaat ttcatccgcg acggtcagcg acgagctgta attgccggcc ggactgtgca
    13261 gcacgaagcc gcgctcggtc tgcaccgggc cgccgaagta gaccggcgcg tccttccacg
    13321 gcgcgatttc gagtttcagg tcgatcttgt cgaacagctt cgccagcgtc aggtcggtcg
    13381 agcgattgat gaccagcccg atcgcgccct tgtcgttgtg ctcgcagacg taaaccactg
    13441 tgcgctcgaa gttcggatcg accagcgtcg gcatcgcgat caggaactga tgcgtcaggt
    13501 tcatctgggc cttggccatg cgacgaattg tattctcgtt cccgcgcgac gacggcgagc
    13561 cgaacgtcgt ctcacagttg ccattcctat ggatgccgcc ctcgtctggt tccgccgtga
    13621 tctgcgcctg cgcgatcacg cggctctgta tcacgcgctg cgcgccgcgc ggcgcgtcta
    13681 ctgcgggttc gtgttcgacc gcgcgattct cgatccgttg ctggcggaag gaatcgtcgc
    13741 cgaccgtcgc gtcgagttca tccatcgcgc cgtgaccgag ctggcgcagg cgctcgatga
    13801 gcgcggcggc gcgctgctcg tgcggcacgg gttcgcgtcc gaggaaatcg ttcgcctcgc
    13861 ggctgaactc catgtcgacg cggttttcgc caatcacgat tacgagcccg atgcgatcgg
    13921 gcgcgaccgc cacgtcgacg ccgagcttgc cgccactggc agacgcctgc tgaccttcaa
    13981 ggatcaggtg atcttcgaga aggaggaggt ggtgacggtc gccggcaggc ccttctccgt
    14041 gtttacgcct taccgcgtcg cgtggctcaa gaagctgacg ccgttctatg tcaaaccgta
    14101 tccggtcgaa cgtcacgcga ctgcgctcgc gcgaccgccg cgcgtcgagc cgttgccgac
    14161 gctggccgag cttggatttg ccggcacgaa cctcggccgc ctgcgcattg ccgcgggcgc
    14221 agcaggcggc gcgcaggcat tcaccgaatt caaggcccgc atcgacgatt acgaccggct
    14281 gcgcgatttt ccatcgcgca acgggccgtc gtatctgtcg gtgcatctgc gcttcggcac
    14341 ggtttcgatc cgcgaactgg cggacttcgc gtccgagcgg gcggcagcgg aatcgcgcgg
    14401 cgcggcgatc tggctgtcgg aactgatctg gcgcgatttc ttccagcaga tcctgtttca
    14461 ccatccgcac gtcgtgagcg gcgccttccg tccggaatac gaccgcatcc gctggcgcga
    14521 cgatcccgaa ctcttggccg cgtggtgcga gggccgcacc ggctacccgt tggtcgacgc
    14581 ggcgatggcg cagatcaacc gcaccggcta catgcacaac cggctgcgca tggtggtcgc
    14641 gagttttctc gtcaaggatc tcggcatcga ctggcggcgc ggcgagcggt acttcgctcg
    14701 ccacctgaac gatttcgatc tggcggccaa caacggcggc tggcagtggg cagcgtcgag
    14761 cggatgcgat gcacagccgt actttcgcat cttcaatccg gtcgcgcaga gcgagaagtt
    14821 cgatccggac ggcgcgttca tccggcgcta cctgccgcag ttggccgggt tcagcccacg
    14881 cgagatccat gcgccatggc gcgtgccgat cgcacggcag cgcgatgccg gatgcgtgat
    14941 cggcgtcgac tatcccgcgc cgatcgtcga ccatgcgcaa gcgcgcgcgc agacgctggc
    15001 gcgctatgca gcagtcaggc gcgcctgatc tcgcgctcga ctgtggcgta gtagaacgca
    15061 acggcgccga gcgacgccac cgcgatcagc accatcgtca cgcggtcgta accgccgagc
    15121 acggtccaca gcagcgccgc caatatcggt ccgaccgcgc gcgcgagcag gccgggcatc
    15181 gcgagcgcgc cggcgatcgc gccgtactgt tcgcgaccat agagctccgc cggtagtgcg
    15241 ccacgcacga cggtcatcgc cccgttgccg gcgccgtaca gcaccgcaaa cagcacgagc
    15301 agcgcggcgc cgccatcggc aacgagcaga gccaggagtg acgccggcag cagcgacgct
    15361 gcgatacgcc ccacccgtcc cgcatgcacc caacggctgg cgccgaattc gacgacgcgt
    15421 ccggcaacct gcatcggacc gatcagcgca ccgatgccgg cagcggcggt cggcgacagg
    15481 ccgcgcgcca gcagcagcgc gatcagatgc gccgccaccg cggacacgac gacgtaatgg
    15541 gccaggaacg cgagcgcaag tgcccggaag cgtcgctcgt gccacggcgg atgcgcggcc
    15601 ttgctgaacg gtgccgattc cgccgcgcgg cctgtccgcg cgcgcggcag cagccaatag
    15661 tgcagcggca cgcagacgag gagattgatc agagccagcc aaagcgtcgc ctcgcgccac
    15721 ccatggcgct cgatcagcca tgtcgtcagt ggccagaaca cggtgctggc gaagccgccg
    15781 aacaacgtca gcaccgtcag cgcacggcgg tatccgagtg cgaagatctg cgtgatcacc
    15841 gcgaaggccg gctcgtacag cgtcgccgcc atcgccacgc cgagccccgc ccagaccacg
    15901 tacaacgcgg ccagggtctc gacgcgcgac agggccgcga gcagcgcggc cgccgccagc
    15961 gaccccagcg tcatcacgcc acgcccgccg agccggtcga tggtcctgcc gattcgtgtc
    16021 gagcacaggc ccgatgcgag cagcgcgacc gagaatgctc cggccaccgc gtccttgccg
    16081 gcgccgaggt cgtgttgcag cggctccagc agcagtgcga agccatagta gatcgaaccc
    16141 cacgagacaa tttgcgtgac gccgagcgcg atcagcaaag gccagccgcg cgaccgtatc
    16201 ggcgccgggg attcgtccgc agtcactgtc cgagcataag gcttgcgcgg cgggggcaaa
    16261 aaaaacggcc cgccagggcg ggccgtttca gcttgccgcg gatcgcggga cttacatgtc
    16321 catcccgccc atgccgccca tgccgcccat gccgccgggc ataccgccgc caccgcctgc
    16381 gggtttctcc tcgaccagct cggcgaccat cgcatcggtg gtcagcatca agcccgcgac
    16441 cgatgcggcg ttctgcaacg ccgtgcgcgt gaccttggtc gggtcgagca cgcccatcgt
    16501 caccagatca ccgtactccc cggtctgcgc gttgaagccg aaattgccct tgccttcggc
    16561 gaccttgttg acgaccacgc ttggctcgta cccgctgttc tcgacgatca tgcgcagcgg
    16621 ctcttccatc gcgcgcagca cgatcttgat gccggcctcc tggtcggtgt tgtcgccctt
    16681 gaccttggag atcgcctgct tggcacgaat cagcgccacg ccgccgccgg caacgatgcc
    16741 ttcctcgacc gccgctcggg tggcatgcag cgcgtcctcg acgcgggcct tcttctcctt
    16801 catctcgacc tcggtggcgg caccgacctt gatcagggcc acgccgccgg ccagcttcgc
    16861 cacgcgctcc tgcagttttt cgcggtcgta gtcgctggtc gcctcctcga tctgggcgcg
    16921 aatcgccttc acgcgcgcct cgatcgcctt ggcatccccg gcgccgtcga tgatcgtcgt
    16981 gttctctttc gcgatctcga tccgcttggc ctggccgagg tcgttcagcg tcgccttctc
    17041 cagcgtcatg ccggtttcct cggcgatcac gttgccaccc gtcaggatcg caatgtcttc
    17101 cagcatcgcc ttgcgacgat cgccgaagcc cggcgccttc accgcgcagg tcttgagaat
    17161 cccgcgaatg ttgttgacca ccagcgtcgc cagcgcttcg ccttcgacct cttcggcgac
    17221 gatcagcagc ggacgccccg ccttggccac ctgctcgaga atcggcagca ggtcgcggat
    17281 gttggagact ttcttgtcgt gcagcagcac gtaggggttg tccaggatcg cgatctgctt
    17341 gtcggcattg ttgatgaagt acggcgacag atagccgcgg tcgaactgca tgccctcgac
    17401 gatctcgagt tcgttgtcga gcgacttgcc gtcctcgacc gtgatcacgc cttccttgcc
    17461 gaccttgtcc atcgccttgg cgatgatgtc gccgatgttc tcgtcggcat tggccgagat
    17521 cgcgccgacc tgggcaattt ccttgttggt cgtgcagggc ttgctgatct tcttcagttc
    17581 ttcgacggcc gccgtgacgg ccttgtcgat gccacgcttc aggtccatcg ggttcatgcc
    17641 ggcggccacg tacttcatgc cttcggcgac gatcgactgc gccaggacgg tcgcggtcgt
    17701 ggtgccgtca cccgcgtcgt cggaagtcct ggaagcgact t
//
LOCUS       DBNHBY010000224         6118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_960188, whole genome shotgun sequence.
ACCESSION   DBNHBY010000224 DBNHBY010000000
VERSION     DBNHBY010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_960188"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16HB_05565"
     CDS             complement(<1..365)
                     /locus_tag="AC16HB_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5606294.1"
                     /translation="MSTRPRILVVEDEPAIADTIVYALATDGFDVQWVATGAEALAAL
                     DKQPCALAILDVGLPDINGFDLFKRVSARHPGLPAVFLTARTAEIDRVVGLELGADDY
                     VAKPFSPRELVARVRAVLR"
     gene            complement(405..1631)
                     /locus_tag="AC16HB_05570"
     CDS             complement(405..1631)
                     /locus_tag="AC16HB_05570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5606295.1"
                     /translation="MSEPPHVDDEAGREAPGGRRALRRQLWLGVAAVALMACGVALAL
                     SALKIEGALHERYDDALSLAGKTLEAQQAAADLRRWQVRFALRIAETGAPGRFEEAFR
                     AVMQTRASLDRLARQLREFDSGSASNHIARIETALDSLAQTNQRVHDDVAARRIAPSA
                     ARDQLLSSTEAALAEIDATLPELVAVTHEHVDQMSRRAQEHGQLARRSLLAVSLIALA
                     LAALLGIVGWRAMRSNARLLAQLEQIAHEDALTGAINRRGLAERLPVELARAGRIAYP
                     LTVAMIDFDHFKRFNDRRGHPAGDRLLRGAVEAWRDRLRPMDMLVRYGGEEFTLVLPA
                     CDAEQTCALIERLRPLMPERQTFSAGVAKWNGTDAADELLRNADAALMQAKKSGRNRT
                     VVWGNEPQITLPLVVR"
     gene            complement(1658..1981)
                     /locus_tag="AC16HB_05575"
     CDS             complement(1658..1981)
                     /locus_tag="AC16HB_05575"
                     /inference="COORDINATES: protein motif:HMM:NF020469.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HIR5606296.1"
                     /translation="MALVVFRSKAAGEIFYLAENARRLFEIIGKEPAPRGVITAAQLP
                     DAIAKLVAAVDEEKAQLKAAQQQADDEQPGGAPRAVTLGQRAYPLLEMLRAAQKRNVD
                     VTWGV"
     gene            complement(2040..2219)
                     /locus_tag="AC16HB_05580"
     CDS             complement(2040..2219)
                     /locus_tag="AC16HB_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606297.1"
                     /translation="MWLAFRSHYPYVALTLVGVPVLLADPALFILGLALAIGLVPCAY
                     AQDVDEIADRTHDQQ"
     gene            2363..2485
                     /locus_tag="AC16HB_05585"
     CDS             2363..2485
                     /locus_tag="AC16HB_05585"
                     /inference="COORDINATES: protein motif:HMM:NF028380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HIR5606298.1"
                     /translation="MGKGDLRTRRGKIYNGSYGKKRPGRVKKKAAAAKTAAHRR"
     gene            2527..3084
                     /locus_tag="AC16HB_05590"
     CDS             2527..3084
                     /locus_tag="AC16HB_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606299.1"
                     /translation="MFVLRGFLSVIAFALLAACTPKFDWREIRSAEGGFAILMPDKPQ
                     TATREISFDGRPIRMTMVSTGVGPTLFAAGVARLPPEALAAPQLDATLARFRDALLHN
                     IDGHATDVRPIVLLPAAAAGHVVHGGQAVEARGRVGKEGRPGQLAARFHVVDDRLYQV
                     VAIGAEGELNADALDTFFSSFRLTQ"
     gene            3095..4432
                     /gene="aptA"
                     /locus_tag="AC16HB_05595"
     CDS             3095..4432
                     /gene="aptA"
                     /locus_tag="AC16HB_05595"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR5606300.1"
                     /translation="MKLENPLTGSMVSLDEWWMPFTANRQFKAAPRLLAKADGMYYWT
                     PDGRQVLDGTAGLWCVNAGHNRKPIVEAVQRQAATMDYAPPFNLGHPVAFEAATAVAA
                     VAPEGMKRVFFTNSGSESADTSIKIALAYHRARGEPGRRIIIGRERGYHGVNVGGTTA
                     GGIPGNRKAFPTLMAGVDHMRATHDLARNAFSRGQPAHGAEFADDLEKLIAFHDASNV
                     AAVIVEPMAGSAGVLIPPKGYLEKLRAVCDKHGVLLIFDEVITGFGRLGAPFAAQRFN
                     VKPDILNCAKAINNAAVPMGAVIVRNEIHDAIVNGAPEGQIEFLHGYTYSGHPLACAA
                     AIATQKLFAEEKLFDRARAMEPVFEAALHSLKGARHVIDIRNLGLVGGIELEPRPGAP
                     GRRALETHIRCFESGALVRFTGDILALSPPLIVEKAEIERLVETIRSVLQALE"
     gene            4521..5000
                     /locus_tag="AC16HB_05600"
     CDS             4521..5000
                     /locus_tag="AC16HB_05600"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR5606301.1"
                     /translation="MSMPSRLSNYLQRCGASYDLCSHPHSRCSAETARAAHVTPHQLA
                     KSVILEDDQGCVMAVVPADARVNVGQLARMLGRDRLHLSDEERIAEMFSDCDLGAVPA
                     IGMAWGMETVVDDELEAGAEVFIEAGDHEMLLRMSREEFRAMMSDARHGHFCSRTIH"
     gene            5010..>6118
                     /gene="mmsA"
                     /locus_tag="AC16HB_05605"
     CDS             5010..>6118
                     /gene="mmsA"
                     /locus_tag="AC16HB_05605"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR5606302.1"
                     /translation="MGKGETTLEDITHWIDGARVGGTPARTGEVFNPATGEVTGLVRF
                     ADAATVDAAVQSAKRAFPAWADTPALRRARVMFRFKELLEKHHEALAAILTREHGKVF
                     EDAKGEVVRGIEVVEFACGIPHLLKGDYSEQVSTGIDQFSLRQPLGVVAGITPFNFPA
                     MVPMWMYPIALACGNAFVLKPSERDPSAALFMADLLKQAGLPDGVFNVVHGDKVAVDA
                     LLTHPDLAAVSFVGSTPIAEYIQQEGTRHGKRVQALGGAKNHMVVMPDADLEQAVDAL
                     IGSAYGSAGERCMAISVCVAVGRIADALVERLAARAQQLKVGDGMDRTSEMGPLVTGA
                     HHAKVKGYVDLGVQEGARLVVDGRRLKVPGREKGF"
BASE COUNT          948 a         2073 c         2154 g          943 t
ORIGIN      
        1 cttcgcagga cggcacggac gcgggcgacc agctcgcgcg gcgagaacgg cttggcgaca
       61 tagtcgtcgg cgccgagttc caggccgacg acgcggtcga tctcggcggt gcgcgcggtc
      121 aggaagaccg cgggcaggcc cggatgccgg gcactgaccc gcttgaacag gtcgaagccg
      181 ttgatgtcgg gcaggccgac gtcgaggatc gccagcgcac acggctgttt gtccagcgcc
      241 gccagcgcct cggcgccggt ggcgacccat tgcacgtcga agccgtcggt cgcgagcgcg
      301 tagacgatcg tgtcggcaat cgccggctcg tcctcgacga ccagaatgcg ggggcgcgtg
      361 ctcatcgcag ccgcgatgat cgcaaatccg cgcggcggcg cgcctcagcg cacgaccagc
      421 ggcagcgtga tctgcggctc gttgccccac acgacggtgc ggttgcggcc cgacttcttg
      481 gcctgcatca gcgcggcgtc ggcattgcgc agcagttcgt cggcggcgtc ggtgccgttc
      541 catttcgcga cgccggccga gaacgtctgc cgctccggca tcaacgggcg gagccgctcg
      601 atcagcgcac aggtctgctc ggcgtcgcac gccggcagca ccagcgtgaa ctcctcgccg
      661 ccatagcgaa ccagcatgtc catcggccgc aagcggtcgc gccacgcctc gaccgcgcca
      721 cgcagcagcc ggtcgccggc cgggtggccg cggcggtcgt tgaagcgctt gaagtggtcg
      781 aagtcgatca tcgccaccgt cagcggatag gcgatgcggc cagcgcgtgc gagttcgacc
      841 ggcaggcgtt cggcgagacc gcggcggttg atcgcgcccg tcaacgcgtc ctcgtgcgcg
      901 atctgctcga gttgcgccag caggcgcgcg ttcgaccgca tggcgcgcca gccgacgatg
      961 ccgagcagtg cggccagcgc cagcgcgatc aggctcaccg ccagcaggct gcgccgcgcg
     1021 agctgcccgt gttcctgcgc gcgccgcgac atctggtcga cgtgctcgtg cgtgacggcg
     1081 acgagctccg gcagcgttgc gtcgatctcc gccagcgccg cctcggtcga cgacagcagc
     1141 tgatcgcgcg cggcgctggg cgcgatgcga cgcgcggcca cgtcatcgtg cacgcgctgg
     1201 ttggtctgcg cgaggctgtc gagcgccgtc tcgatccgcg cgatgtggtt cgacgcgctg
     1261 ccggagtcga attcgcgcag ttggcgcgcc aggcgatcga gcgaagcgcg cgtctgcatc
     1321 accgcccgaa acgcttcctc gaagcggccc ggtgcgccgg tttcggcgat gcgcagcgcg
     1381 aagcggacct gccagcggcg caggtcggcc gctgcctgct gcgcctcgag tgtcttgccc
     1441 gcgagcgaca gcgcatcgtc gtagcgctcg tgcagcgcgc cttcgatctt cagcgcggag
     1501 agcgccagcg ccacgccgca ggccatcagc gcgactgcgg cgacgccgag ccagagttgc
     1561 cgccgcagcg cccgtcgtcc gcccggcgcc tcgcgcccgg cttcgtcatc gacgtgaggc
     1621 ggctcgctca ccgcggctcc cgacccggtc tgtcggctca gacgccccaa gtcacgtcga
     1681 cattcctctt ctgcgccgcg cgcagcattt cgagcagcgg ataggcgcgc tgtcccagcg
     1741 tcaccgcgcg cggagcgccg ccgggctgct catcgtccgc ctgctgctgg gccgccttca
     1801 actgcgcctt ctcctcgtcc actgcggcga ccagcttggc gatcgcgtcc gggagctgcg
     1861 cggccgtaat cacgccgcgc ggcgccggct ccttgccgat gatctcgaac agccggcgcg
     1921 cattctcggc gagatagaag atctcgcccg cggccttgga gcggaagaca accagcgcca
     1981 cgacttcctc ccatgttgtc ggtgtcgggc gattgtaggg tgccgatcgt cgcccactct
     2041 cactgctgat cgtgcgtgcg atcggcgatc tcgtcgacgt cctgcgcgta ggcgcacggc
     2101 accagcccga tcgcgagcgc gaggccgagg atgaacagcg ccggatcggc cagcagcacc
     2161 ggcaccccga ccagcgtcag cgccacatac gggtagtgac tcctgaatgc cagccacatt
     2221 gccacctccc cggaccgcca gcgtccgcgc gtctgatcga acgattctgc gccgacaccg
     2281 gcagggcgcg tgatgcggac attccggatc ccgtttccgg gctacctata atccgcgctc
     2341 gactcgaacg agggagtttc agatgggcaa aggcgacctg cgcacgcggc gcggcaagat
     2401 ctacaacggc agctatggaa agaagcggcc gggccgcgtg aagaagaagg cggccgctgc
     2461 caagacggcc gcgcaccgca ggtagccgcg cgcgatccgg acgagccggg ccgcgggccc
     2521 ggttccttgt tcgtgctgcg cgggttcctg tccgtcatcg cctttgcgct gttggcggca
     2581 tgcactccga aattcgactg gcgcgagatc aggtcggccg agggcgggtt tgcgatcctg
     2641 atgccggaca agccgcagac cgccacgcgc gagatttcat tcgacgggcg accgatccgg
     2701 atgaccatgg tgtcgaccgg cgttggtccg acgctttttg ccgctggggt cgcgcggttg
     2761 ccgccggagg cgctcgccgc gccgcaactc gacgcgactc tggcgcgctt tcgcgacgcg
     2821 ctgctgcaca acatcgacgg ccacgcgacc gacgtgcgcc cgatcgtgct gctgccggcc
     2881 gcggcggctg gtcatgtggt acacggcggg caggctgtcg aggcgcgcgg gcgggtcggc
     2941 aaggaagggc gacccggaca acttgccgcg cgcttccatg tggtcgatga tcgcctgtac
     3001 caagtggtgg ccatcggcgc cgaaggcgaa ctgaacgccg acgcgctcga cacctttttc
     3061 tcttcctttc gcctcaccca ataagggggc ctgaatgaag ctcgagaatc cgctgaccgg
     3121 atcgatggtt tcgctcgacg aatggtggat gccattcacc gcgaaccggc agttcaaggc
     3181 cgcgccgcgg ctgctggcga aggccgacgg catgtactac tggacgccgg atggccgcca
     3241 ggtgctcgac ggcaccgcgg gactgtggtg cgtgaacgct ggccacaacc gcaagccaat
     3301 cgtcgaggcg gtgcagcggc aggcggcgac gatggactac gcgccgccgt tcaacctcgg
     3361 gcacccggtc gccttcgagg ccgctaccgc ggtcgccgcg gtcgcgccgg aggggatgaa
     3421 gcgggtgttc ttcaccaact ccggctcgga gtcggccgac acctcgatca agatcgcgct
     3481 cgcctatcac cgcgcgcgcg gtgaaccggg ccgccgcatc atcatcggcc gcgagcgcgg
     3541 ttaccacggc gtcaacgtgg gcggcaccac ggcgggcggc atccccggca accgcaaggc
     3601 gttcccgacg ctgatggcgg gcgtcgatca catgcgcgcc acgcacgatc tggcgcgcaa
     3661 cgcgttttcg cgcgggcagc ccgcgcatgg cgccgaattt gccgatgacc tggaaaagct
     3721 gatcgccttc cacgacgcca gcaacgtcgc cgcggtgatc gtcgagccga tggctggctc
     3781 ggccggcgtg ctgattccgc ccaagggcta cctggagaag ctgcgggccg tctgcgacaa
     3841 gcacggcgtg ctgctgatct tcgacgaggt gatcaccggg ttcggccggc tcggcgcccc
     3901 gttcgccgcg cagcgcttca acgtcaagcc cgacatcctg aactgcgcca aggcgatcaa
     3961 caacgcggcg gtgccgatgg gcgcggtgat cgtgcgcaac gagatccacg acgccatcgt
     4021 caacggcgca ccggaaggcc agatcgagtt cctgcacggc tacacgtatt cgggtcaccc
     4081 gttggcgtgc gcagcggcga tcgccacgca gaaactgttc gccgaggaga agctgttcga
     4141 ccgcgcgcgc gcgatggagc cggtgttcga ggctgcgctg cattcgctca agggagcgcg
     4201 ccacgtgatc gacatccgca acctggggct ggtcggcggc atcgagctgg agccgcggcc
     4261 gggagcgccg ggccggcgcg cgctggagac gcacatccgc tgcttcgagt ccggcgcgtt
     4321 ggtgcgcttc acgggcgaca tcctggcgct gagcccgccg ctgatcgtcg agaaggccga
     4381 aatcgagcgg ctggtcgaga cgatccgttc tgtgctgcag gcactcgaat agcacctatc
     4441 ggggtgctgg cgcccactgg cgcgcgggag cagactttgc gtgctgcgcc atcctgcccg
     4501 gcgcgtcaga ggagactgcc atgtcgatgc catcacgcct gtcgaactac ctgcaacgct
     4561 gcggggccag ctacgacctg tgctcgcacc cgcacagccg ctgcagcgcg gagactgcgc
     4621 gcgcggcaca tgtcacgccg catcagctcg ccaagtccgt gatcctggag gacgaccagg
     4681 gctgcgtgat ggcggtggtg ccggccgatg cgcgcgtcaa cgtggggcag ctcgcgcgca
     4741 tgctggggcg cgatcgactc cacctgtccg acgaggagcg catcgccgag atgttctccg
     4801 actgcgatct gggcgcggtg cccgcgatcg gcatggcatg gggcatggaa acggtcgtcg
     4861 acgacgaact ggaagccggt gcggaggtgt tcatcgaggc cggcgatcac gagatgctgc
     4921 tcaggatgtc gcgcgaggaa ttccgcgcga tgatgagcga cgcgcgccac ggacacttct
     4981 gcagtcggac gattcactga gccggactca tgggaaaagg cgaaaccact ctcgaagaca
     5041 tcacgcactg gatcgacggc gcgcgcgtcg gcggcacgcc ggcgcgcacg ggtgaggtgt
     5101 tcaaccccgc aaccggggaa gtgaccggcc tggttcggtt cgccgatgcg gcgacggtcg
     5161 acgccgccgt gcagtcggcg aagcgtgcgt tcccggcatg ggccgacacg cctgcactgc
     5221 ggcgcgcgcg tgtgatgttc aggttcaagg aactgctgga gaagcaccac gaggccctgg
     5281 cggcgatcct gacgcgcgag catggcaagg tgttcgagga cgccaagggc gaggtggtgc
     5341 gcggcatcga ggtggtcgag ttcgcgtgcg gcattccgca cctgctgaag ggcgactact
     5401 cggagcaggt gtccaccggc atcgatcaat tctcgctgcg acagccgctc ggcgtggtcg
     5461 ccggcatcac gccgttcaac ttcccggcga tggtgccgat gtggatgtat ccgatcgcgc
     5521 tcgcgtgcgg caacgcgttc gtgctcaagc cctccgagcg cgatccttcg gctgcgctct
     5581 tcatggccga cctgctgaag caggcggggt tgcccgacgg cgtgttcaac gtcgtgcacg
     5641 gcgacaaggt cgcggtcgat gcgctgctta ctcaccccga tctcgcggcg gtcagcttcg
     5701 tcggctccac gccgattgcc gaatacatcc agcaggaagg cacgcggcac ggcaagcgcg
     5761 tgcaggcgct cggcggtgcg aagaaccaca tggtcgtgat gccggacgcg gacctcgagc
     5821 aggcggtcga cgcattgatc ggatcggcat acggctcggc gggcgagcgc tgcatggcga
     5881 tttcggtgtg cgtggcggtc gggcgcatcg ccgatgcgct ggtcgagcgg ctcgccgcgc
     5941 gtgcgcagca actgaaggtc ggcgacggca tggaccggac gtccgagatg gggccgttgg
     6001 tcaccggcgc gcatcacgcg aaggtcaagg gctacgtcga tctcggcgtg caggagggcg
     6061 cgcggctcgt cgtcgacggc cgcaggctga aggtgccggg gcgcgagaag ggcttctt
//
LOCUS       DBNHBY010000225         7919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_147911, whole genome shotgun sequence.
ACCESSION   DBNHBY010000225 DBNHBY010000000
VERSION     DBNHBY010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_147911"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..826
                     /locus_tag="AC16HB_05610"
     CDS             188..826
                     /locus_tag="AC16HB_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606303.1"
                     /translation="MAGALPLFATVIAYALAIRLELAPACNPFLEGCVSISRAARHDL
                     PNILFRALILPAAVLQIACWLLCPAWLRSIDAKPDGWLRALPWLGVGAGVALILYGAF
                     LGTEGEGYRFLRRYGTTFYFGFTCIAMLLVTRGMRRLSHTVTRLLTALCMALPLLGLV
                     HVVLPIALSGENARNAVQNATEWWGGAIFTLFFVVLAWAWRVTAFRARFGRG"
     gene            912..2135
                     /locus_tag="AC16HB_05615"
     CDS             912..2135
                     /locus_tag="AC16HB_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5606304.1"
                     /translation="MIDRRRFIGGATASGAGALLPAWARAATAELGFAELPAGQVSAQ
                     ALEALPGKVALIRKSYRPPNYETPVEYLGDVYTRNDAFFVRWHLANIPEVKADQWRLN
                     VGGEAAERSVSFTLQELKTQFQQTEVVAVCQCSGNRRGLFEPHVPGVIWGPGAMGNAR
                     WRGVRLKDVLARAGVKKEAVEISFDGADAGAIAATPDFVKSLPPWKALDDDTLIAFEM
                     NGAPLPHLNGYPARLIVPGWTATYWVKQLTDIAVLAKPFTGFWMATAYRVPVGKFPTV
                     DRFITQETAVNTPITQMVTNSLITNLRVGQRVNAAQGLEVRGVAWDAGSGIVAVDVSL
                     DGGASWQRSALGENVGRYSFRTFALRVPKLAPGSYEVLARAESANGTTQTFTLIHNPA
                     GYHHNVVQRIPIVAA"
     gene            2152..2442
                     /locus_tag="AC16HB_05620"
     CDS             2152..2442
                     /locus_tag="AC16HB_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR5606305.1"
                     /translation="MKRWMGIALAALPLAAAADEAQIALKDAPGRNVLLANCTMCHSV
                     DYVQGNSPFPDRKMWEAEVAKMRNAFKAPISDADARTIVDYLVANYSSEAKK"
     gene            complement(2449..3570)
                     /locus_tag="AC16HB_05625"
     CDS             complement(2449..3570)
                     /locus_tag="AC16HB_05625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR5606306.1"
                     /translation="MSRPQRRRDRSRHSRPRRTSPGEAAPGASVPRYHRRLEATQDND
                     SPDRQTAPFADLTPDAVLNAIDAVGLASSGTLLALNSYENRVFQLGVEDAQRFVVAKF
                     YRPARWTDAQILEEHAFTLELANAEIPAVPPLALDGQTLHARAGYRFALYPRRGGRAP
                     ELEDRDVRLWLGRFIGRIHAMGGTKRYAHRPALDVATFGEEPSAYLIDSGAVPHALSD
                     VYSGVVAQALDEVRRCYERAGAVRTLRLHGDVHVGNVLWTDAGPHFVDFDDSRMGPAV
                     QDLWMLLSGDREAMQAQLADLLAGYEEFMDFDRRELQLVEALRTLRLIHYAAWLARRW
                     DDPAFPAAFPWFDTPRFWEARVLELREQVAAMQDTPLAA"
     gene            complement(3503..3790)
                     /locus_tag="AC16HB_05630"
     CDS             complement(3503..3790)
                     /locus_tag="AC16HB_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606307.1"
                     /translation="MTLRTLHCAAVSAALAAAGAAQAQERCDPPHDMLDARACAKAAE
                     GPVALRRFVERTRMLWNLYYWDYAPRDADDKSATTAARPQPTQSTAADKSG"
     gene            complement(3889..4056)
                     /locus_tag="AC16HB_05635"
     CDS             complement(3889..4056)
                     /locus_tag="AC16HB_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606308.1"
                     /translation="MNRAFNPTSRSIRSLLAVAAVAVTALIAGSIAGLVQHYEAAAQV
                     AALPGVVVAAR"
     gene            complement(4399..4962)
                     /locus_tag="AC16HB_05640"
     CDS             complement(4399..4962)
                     /locus_tag="AC16HB_05640"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5606309.1"
                     /translation="MLASELRTGTVLRFEGDFYRVIDASGHAGGGQLGGVMHAKLLNL
                     RSRHAVERRFRPDERLEPVDVDRARWQFIYADGDDYYFMNPETYEQIPIPGAALGAAK
                     AFVAPGLEATVESHEGRPLYVVLPDAVELQIAETAEPTHQRDTSAMKTAKLDNGVEVL
                     VPLFIKTGDRVRIDTASGKYLERAKAK"
     gene            5123..7540
                     /locus_tag="AC16HB_05645"
     CDS             5123..7540
                     /locus_tag="AC16HB_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606310.1"
                     /translation="MSTVTALTSTAYGFAGFELKPQERALLQAGTPVKLGARAFDVLM
                     ALIERRDRVVTKDELFDIVWPGLVVEENNLQVQVSSLRKLFGPHVIATVPGRGYQFVS
                     PLQTPAANEAPAHATPNNLPQQRTHFIGRGAELAQCTRLLRDTRLLTLTGVGGCGKTR
                     LALQFAQQQLDGFPDGVWFVDLAPLQEAGRVAIPLATVLGVLEVAGKSVVERLIEHLS
                     SRRALFVLDNCEHVIDAVAGLAETLLTRCAGLKFVVTSREGLGLAGEQIFSVRPLSLP
                     ATADVETMNASEAVRLFVDRARLAIPELVVDERNAAVIADICRRLDGIALALELAAAR
                     VAMLSFEEIHDRLDDRFRLLTGGSRALPRQQTLQATMQWSHDLLAPEEQRLFRQLAVC
                     SGGWTLPAAAHITDAADEYEVLHLLTRLHDKSLLQVDRDGAGQPRYRMLETVRQYAQE
                     RLNDSGESDAVRTRHLHYFVALAEEAARNMPGPHEGVWMARLRLEQEDLLAAHAWAEH
                     APDAESALRLVAALRHYLANSDQIVCGYRLARSALALDGAGVGASPRAYALLAASKFA
                     YFLGKYADALRYAEQALELARSAGDADLNLKALRWLASTLEATGDTERARQYLEEVRD
                     AARSAGKHADLSATLIGLGELHRAAGDLAQAVACYEESIALDRAAQSSCGMANGLGNL
                     ARALVLLGLLERARVATLECLQVAEAAGQRATCACALDPVAGVAESLGDHARSARYYG
                     AANALRRESGTLREPVDQAFIDTLIARTRTALDAAAFAAAEEEGSAMSFEAAIADSQH
                     WLARAAH"
     gene            7609..>7919
                     /locus_tag="AC16HB_05650"
     CDS             7609..>7919
                     /locus_tag="AC16HB_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5606311.1"
                     /translation="MPAQEYLRRILTARVYDVAIESPLEEAPKLSERLGNRVLLKRED
                     LQPVKSFKLRGAYNKMARLPRDELKRGVIAASAGNHAQGVAMAAQKLGCRAVIVMPVT
                     TP"
BASE COUNT         1213 a         2634 c         2775 g         1297 t
ORIGIN      
        1 ttcggcggca gcggcgcatt catcgtggtc gatcggcaag cggcccacgg tcaacggcgg
       61 cgcggctcga ggtggaagtt tatctgttgc atcgcggcga aacgcgcacg atcatcaagg
      121 aaaagcctgg cctcggggaa tgcagcccgt ttccgcccgc gatcgtccgc tctggccgct
      181 gccgctcgtg gccggcgcgc tgccgttgtt cgcgaccgtg atcgcgtatg cgctcgcgat
      241 ccgactcgaa ctggcacccg cgtgcaatcc gttcctcgaa ggctgcgtgt cgatcagccg
      301 cgcggcacgc cacgatctgc ccaatatcct gttccgcgca ctgattctgc cggctgcggt
      361 gctgcagatt gcctgctggc tgctgtgccc ggcgtggttg cggtcgatcg atgcgaagcc
      421 ggatggctgg ctgcgggcat tgccgtggct gggggtgggg gccggcgttg cactgatcct
      481 gtacggcgcc ttcctgggca cggagggcga gggctatcgc ttcctgcgtc gctacggcac
      541 gaccttctat ttcggcttca cctgcatcgc catgctgctt gtcacccgcg ggatgcggcg
      601 actctcgcac accgtgacgc ggctactgac cgccctgtgc atggcactgc cgctgctcgg
      661 cctcgtgcac gtggtgctcc cgatcgcgct gtccggcgag aacgcgcgca acgcggtgca
      721 gaacgcgacc gagtggtggg gtggtgcgat ctttaccctg ttcttcgtcg tcctcgcatg
      781 ggcgtggcgc gtgacggcgt ttcgcgcccg gttcggcagg ggctgaccgc atacgggctt
      841 cccggattgt tgcgttggcc ggacgtggct ataagttcgg tcgacaccga caacaatcaa
      901 agggagggac gatgatcgat cgacgcaggt tcatcggcgg cgcgaccgcg agcggagcgg
      961 gcgcgctgct gccggcctgg gcgcgcgcag caacggcgga gctcggcttt gccgagctgc
     1021 cggcgggcca ggtcagcgcg caggcgctcg aagcgctgcc cggcaaggtg gcgttgatcc
     1081 gcaagagcta ccggccgccg aactacgaaa cgccggtcga gtatctgggc gacgtttaca
     1141 cgcgcaacga cgcgttcttc gtgcgctggc atctcgcgaa catccccgag gtgaaggccg
     1201 accaatggcg cctgaacgtc ggcggcgaag ccgccgagcg gtcggtgagt ttcacgctgc
     1261 aggagctgaa gacgcagttc cagcagaccg aagtcgtcgc ggtgtgccaa tgctcgggaa
     1321 atcgcagggg cttgttcgaa ccgcacgtgc ccggcgtgat ctggggcccc ggcgcgatgg
     1381 gcaatgcgcg ctggcgcggg gtgcggctga aggatgtgct ggcgcgcgcc ggcgtgaaga
     1441 aggaagcggt cgaaatctcg ttcgacggcg ccgatgccgg cgcgatcgcc gccacgcccg
     1501 acttcgtcaa gagcctgccg ccgtggaagg cactcgacga cgacacgctg atcgcgttcg
     1561 agatgaacgg tgcaccgttg ccgcatttga acggctatcc ggcgcggctg atcgtgccgg
     1621 gctggaccgc aacctactgg gtcaagcaac tgaccgatat cgcagtactg gcgaagccat
     1681 tcaccgggtt ctggatggcg accgcgtatc gcgtgccggt cgggaagttc ccgacggtcg
     1741 atcgtttcat tacgcaggag accgccgtca acacgccgat cacgcagatg gtgaccaact
     1801 cccttatcac caacctgcgc gtcggccagc gggtcaatgc cgcgcagggg ctcgaagtgc
     1861 gcggcgtggc gtgggatgcc ggttcgggca tcgtcgccgt cgacgtgtcg ctagacggcg
     1921 gcgccagctg gcaacgcagt gcgctcggcg agaacgtcgg acgctactcg ttccgcactt
     1981 tcgcgctgcg cgtaccgaag ctggcaccgg gcagctacga ggtgttggcg cgcgcggaga
     2041 gcgccaacgg cacgacgcag accttcacgc tgatccacaa cccggccggc tatcaccaca
     2101 acgtcgtgca gcggattccg atcgtggcgg cctgagacga tgagaaccga catgaaacgc
     2161 tggatgggaa tcgcactggc tgcgctgccg ctcgcggcgg ccgccgacga agcgcagatc
     2221 gcgctgaagg acgcgcccgg tcggaacgtg ctgctcgcca actgcacgat gtgccacagc
     2281 gtcgactacg tgcaggggaa ctcaccgttc cccgatcgca agatgtggga ggccgaggtg
     2341 gcgaagatgc gcaacgcctt caaggcgccg atctccgacg ccgacgcgcg cacgatcgtc
     2401 gactacctgg tcgccaatta cagcagcgaa gcgaagaagt agtccgcgtc aggccgcgag
     2461 cggcgtgtcc tgcatcgctg cgacctgctc gcgcagttcg agcacgcgcg cttcccagaa
     2521 gcgcggcgtg tcgaaccacg ggaacgccgc cgggaacgcc ggatcgtccc agcggcgcgc
     2581 gagccacgcc gcgtagtgga tcaggcgcag cgtgcgcagc gcttcgacca gctgcaactc
     2641 gcggcggtcg aagtccatga actcctcgta accggccagc aggtccgcca gctgcgcctg
     2701 catcgcctcg cggtcgccgg acagcagcat ccacaggtcc tgcacggccg gacccatacg
     2761 gctgtcgtcg aagtcgacga agtgcggccc ggcgtcggtc cacagcacgt tgccgacgtg
     2821 gacgtcgccg tgcaggcgga gcgtgcgcac ggcgccggcg cgctcgtagc agcgccgcac
     2881 ctcgtcgagc gcctgcgcca cgacgccaga gtagacgtcg gacagtgcgt gcggcaccgc
     2941 accgctgtcg atcagatacg cgctcggttc ttcgccgaac gtcgccacgt cgagcgccgg
     3001 tcgatgtgcg tagcgcttcg tgccccccat cgcatggatg cggccgatga aacggccgag
     3061 ccacagacgc acgtcgcggt cctcgagttc gggcgcgcgc ccaccgcggc gcggatacag
     3121 cgcaaagcgg taacccgcac gcgcatgcag cgtttgcccg tcgagcgcga gcggcggcac
     3181 cgccgggatt tcggcgttgg ccagctccag cgtgaaggcg tgctcctcca ggatttgcgc
     3241 gtcggtccag cgtgccgggc gatagaactt ggcgaccacg aagcgctgcg cgtcctcgac
     3301 cccgagctgg aacacccggt tctcgtagct gttcagcgcc agcagcgtcc cgctgctggc
     3361 gagcccgacc gcatcgatcg cgttgagcac cgcgtcgggc gtcaggtcgg cgaatggcgc
     3421 ggtctgccga tccgggctgt cgttgtcttg cgttgcctcc agtcgccgat ggtatcgggg
     3481 aaccgacgcg cccggcgccg cctcacccgg acttgtccgc cgcggtcgac tgtgtcggct
     3541 gcggtcgcgc cgccgttgtg gccgacttat cgtcggcgtc gcgcggcgca tagtcccagt
     3601 agtacaggtt ccacagcatg cgcgtgcgct cgacgaagcg gcgcagcgca acggggccct
     3661 ccgcggcctt ggcgcaggcg cgcgcgtcga gcatgtcgtg cggcggatcg cagcgctcct
     3721 gcgcttgcgc ggcgccggcc gcagcgaggg ccgccgacac ggcagcacag tgaagggttc
     3781 gaagcgtcat gagggtctcc tttgcgtgcg cgccgcccac ttgcgcgact ggcgcgatgc
     3841 tgccagtttg cggatgccgg caacggtgcg tcgagtccgg ctcctgcttc agcgcgcagc
     3901 gaccacgacc cctggcaagg cggcgacctg cgcagcggct tcatagtgct gcaccagccc
     3961 tgcgatcgag cccgcgatca gcgccgtgac ggcaaccgcg gcgacggcga gaagggagcg
     4021 gatcgagcgg gacgtcgggt tgaatgcgcg gttcatctgg gtctccgggg gtgggcgttg
     4081 acgacggcgc cactgtgcgc cccgcccccc gattcagtcc tttacccgcc tgaagccgcc
     4141 gcttaaacgt tatgaattcg tctggcgccg gccttgagcc gggcgtggcg tggcgctcta
     4201 atgggctgcg caggcagggg atgcagatgt cagatgtaca ccgcttcggc cgcgtcgagg
     4261 tccaccccgg cgcgcggcag ctctgggtcg acggtcgacc cgtcgcgttg ggagcgcgtg
     4321 ccttcgacct gttgctggcg ctcatcgaac ggcgcgaccg cgtggtcggc aagaacgaac
     4381 tctcgacctt gtcttcggtt acttcgcctt cgcccgctcc agatacttcc cgctcgccgt
     4441 gtcgatgcgt acgcgatcgc cggtcttgat gaacagcggc accagcactt cgacgccatt
     4501 gtcgagcttc gcggtcttca tcgcgctggt gtcgcgctgg tgcgtcggct cggccgtctc
     4561 ggcgatctgc agttcgaccg cgtccggcag cacgacgtac agcggccggc cttcgtggct
     4621 ctcgaccgtc gcttccagtc ccggagcgac gaacgccttt gcggcgccga gcgcagcgcc
     4681 cgggattgga atctgctcgt acgtttcggg attcatgaag tagtagtcgt cgccgtcggc
     4741 gtagatgaac tgccagcgtg cgcgatcgac gtcgaccggc tcgagccgct cgtccggacg
     4801 gaagcggcgc tcgaccgcgt ggcgcgaacg caggttcagc agcttcgcgt gcatgacgcc
     4861 gccgagttgc ccgccaccag cgtgtcccga ggcatcgatc acgcgataga agtcgccttc
     4921 gaagcgaagg acggtgcccg tgcgcagttc ggaagccagc atggtcggct cctttcccat
     4981 ttcgccatta tgttcatctc agccaggatt gtcggggctg gccttaaacg ttcttaattc
     5041 tgcgtccgtg cgcagtccgg acgatggcgg cgcgcccggc ccgcgggtaa tctggtccgc
     5101 gatccgcaac aggtgcacct gcatgtcgac cgtgacggct ctgacctcca ctgcgtacgg
     5161 attcgccggc ttcgagctga agccgcaaga gcgcgccctc ctgcaggcag gcacgccggt
     5221 caagctcggc gcgcgcgcgt tcgacgtgct gatggcgctg atcgagcgcc gcgaccgcgt
     5281 cgtgaccaag gacgagttgt tcgacatcgt ctggcccggt ctcgtcgtcg aggagaacaa
     5341 cctgcaggtg caggtgagca gcctgcgcaa gctgttcgga ccgcacgtga tcgcgaccgt
     5401 tcccggtcgc ggctatcagt tcgtgtcgcc gctgcagacc cccgcggcga acgaggcgcc
     5461 ggcgcacgcg acgcccaaca acctgccgca gcagcgtacg cacttcatcg gccgcggcgc
     5521 agagctcgcg cagtgcacgc gtctgctgcg cgacacgcga ctgctgacgc tcaccggcgt
     5581 gggcggctgc ggcaagacgc ggctggcgct gcagttcgcg cagcagcagc tcgatggctt
     5641 tccggatggc gtgtggttcg tcgacctggc gccgctgcag gaggccgggc gcgtcgcgat
     5701 cccgctggcc accgtgctcg gcgtgcttga ggtcgccggt aaatcggtgg tcgagcgcct
     5761 gatcgagcat ctgtcctccc ggcgcgcgct gttcgtgctc gacaactgcg agcacgtgat
     5821 cgacgcggtg gccggcctgg ccgaaacgct gctcacgcgc tgcgcggggc tgaagttcgt
     5881 cgtcaccagc cgcgaggggc tgggcctcgc aggcgagcag atcttctcgg tgcggccgct
     5941 gtcgctgccg gccaccgccg atgtggaaac gatgaacgcg tcggaagcgg tgcgcttgtt
     6001 cgtcgaccgc gcgcggcttg caattccaga acttgtggtg gacgagcgta acgccgcggt
     6061 gatcgccgac atctgccgcc ggctcgacgg cattgcgctg gcgctcgaac tagcggcggc
     6121 acgcgtggcg atgctgtcgt tcgaggagat ccacgaccgc ctcgacgatc gcttccgcct
     6181 gctcaccggc ggcagccgcg cgctgccgcg ccagcagacg ctgcaggcga cgatgcagtg
     6241 gagccacgat ctgctggcgc cggaggagca gcggctgttc cgtcaactcg cggtgtgctc
     6301 cggcggctgg acattgccgg cggcggcgca catcacggat gcggccgacg aatacgaggt
     6361 gctgcacctt ctgacgcgct tgcacgacaa atcgctgctg caggtcgatc gcgatggcgc
     6421 cgggcagccg cgctaccgga tgctggagac cgtacgccag tatgcgcagg agcgattgaa
     6481 cgattccggc gagagcgatg cagtccggac ccggcatctg cactatttcg ttgccctggc
     6541 cgaggaagcc gcgaggaaca tgcccggtcc gcacgagggc gtctggatgg cgcgtctgcg
     6601 gctggagcag gaagacctgc tcgcggcgca tgcgtgggcc gagcatgcac cggacgccga
     6661 atcggcgctg cggctggtcg ctgcgctgcg gcattatctg gcgaattccg atcaaatcgt
     6721 ctgcggctac cggctggcgc gcagcgcact ggccctcgac ggcgcgggcg tcggtgcgtc
     6781 gccgcgcgcc tacgcattgc tcgcggccag caagtttgcg tacttcctgg gcaagtatgc
     6841 cgatgcattg cgctacgcgg agcaggcgct ggaactggcc cggtcggccg gcgacgctga
     6901 tctgaacctc aaggcattgc gttggctggc tagcacgctg gaagcaacgg gagataccga
     6961 gcgcgcgcgg caatacctcg aggaagtacg cgatgccgcg cgctccgccg gcaagcatgc
     7021 ggatttgagc gcaaccttga tcggactcgg cgaacttcac cgcgcggcag gtgacctcgc
     7081 gcaagcggtg gcctgctacg aggaatcgat cgcgctggat cgcgcggcgc agtcgagttg
     7141 cggcatggcg aatggcctcg gcaatctggc ccgggcgctg gtgctgctgg gcctgctcga
     7201 acgggcccgc gtcgcgacgc tcgaatgcct gcaggtcgcg gaggcggccg ggcagagagc
     7261 gacctgcgcg tgcgcgctcg atccggttgc cggggtcgcc gagtcgctcg gcgatcacgc
     7321 gcgctccgcg cgctactacg gcgccgcgaa cgccctccgc cgcgagagcg gcacgctgcg
     7381 cgagccggtc gaccaggcgt tcatcgacac gctgatcgcg cgcacgagga ccgcgctgga
     7441 cgcggccgcc ttcgccgctg ccgaggagga gggatccgcg atgagcttcg aggcggcgat
     7501 tgccgactcg cagcactggc tcgcgcgcgc cgcgcactga ctcgggcgcg gcgccggtcc
     7561 ggctttcccc gcgtgttacc tttcgcgcga gcgaggggga cgagagtaat gcccgcccag
     7621 gaatatctgc gccggatcct gaccgcgcgc gtgtacgacg tggcgatcga atcgccgctg
     7681 gaggaagcgc cgaagctttc cgagcggctc ggcaaccgcg tgctgctgaa gcgcgaggat
     7741 ctgcagccgg tcaagagctt caagctcaga ggcgcctaca acaagatggc gcggctgccg
     7801 cgcgacgagc tgaagcgcgg cgtgatcgcc gccagcgcgg gcaatcacgc gcagggcgtg
     7861 gcaatggcgg cacagaagct cggctgccgc gcggtgatcg tgatgccggt gacgacgcc
//
LOCUS       DBNHBY010000226         2620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1637025, whole genome shotgun sequence.
ACCESSION   DBNHBY010000226 DBNHBY010000000
VERSION     DBNHBY010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1637025"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="aroC"
                     /locus_tag="AC16HB_05655"
     CDS             <1..847
                     /gene="aroC"
                     /locus_tag="AC16HB_05655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5606312.1"
                     /translation="EDPKSRDYEDLVDTFRPGHADYTYWRKYGLRDPRGGGRSSARLT
                     APMVAAGAVARKWLREKFGTRVRGCMQQLGPIAVAFESWEHVDANPFFAPNAAIVPRL
                     EESMDALRKRGDSCGARIRVTATNVPVGLGSPLYDKLDADIAYALMGINAVKGVEIGA
                     GFGAVTQLGSEHGDELTPAGFVSNHAGGMLGGISTGQDIEVSIAIKPTSSIRIARRSI
                     NQRNEAVSIETHGRHDPCVGIRATPIAEAMLALVLIDHALLDRAQNADVVHAVAPIAG
                     AVEKG"
     gene            847..2025
                     /locus_tag="AC16HB_05660"
     CDS             847..2025
                     /locus_tag="AC16HB_05660"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606313.1"
                     /translation="MFRRLPPADWEPWRPALFFGAYFAAAGAFMPYFPLYLEHRGIVA
                     GEIGVLMATAQATRIFGPAFWGWLADHTLQRLRIIRWTAVATALLFALLLAPGGFWWL
                     LPVFFATQFCGTGQVPVCEAIIAARLRGDAHAPARYGRLRVWGSIGFVGTVLALGPVL
                     DRFGIGVQPWLVVLLLALTALATFLLRDAPHAFAPHERVSVRARLAEPRVRWFFASVV
                     LMIFAHGALYSYLSLHLAQLGYSKTEIGVLWVLGVVIEIGFFASQGRFFRRYGVYPLL
                     TATFVLAAIRFALIAQFAHVVVLLVIAQLLHATTFAVHHSASILTVQRWFTGATAARG
                     QALYVSLGYGIGGTAGSLGAAWLWSSIGPSSAFFASSVAALLGWGAVRRSHQLDLQAA
                     "
     gene            2041..>2620
                     /locus_tag="AC16HB_05665"
     CDS             2041..>2620
                     /locus_tag="AC16HB_05665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5606314.1"
                     /translation="MRRMFRRRVAWIAFGVAAVVAGCETVQTTRGGTVGVTREQQMSP
                     LVSRAQVDTAAKQQYVSVLKESGQKGQLNRDSTQTERVRRIAQRLIPQTAVFRDDAPA
                     WQWEVNVITSDQVNAWCMPGGKIAVYTGLIDKLKVSDDELAAVVGHEIAHALREHARE
                     RMGRQMATEATTAIGAAALEIFTGVRIDSQVAG"
BASE COUNT          375 a          832 c          959 g          454 t
ORIGIN      
        1 cgaggacccg aagagcaggg actacgagga tctcgtcgac acgttccggc ccggtcacgc
       61 cgactacaca tactggcgca agtacggcct ccgcgatccg cgcggcggcg ggcgctcgtc
      121 ggcgcggctg accgcgccga tggtcgcggc cggcgccgtg gcaaggaaat ggctgcgcga
      181 gaagttcggc acacgcgtgc gcggctgcat gcaacagctc gggccgatcg ccgtggcgtt
      241 cgagtcgtgg gagcatgtcg atgcgaatcc gttcttcgcg ccgaacgcgg cgatcgtgcc
      301 gcggctcgag gagtccatgg atgcgctgcg caagcgcggc gattcgtgcg gcgcgcgcat
      361 ccgcgtgacc gcgacgaacg tgccggtcgg gctaggaagc ccgctgtacg acaagctcga
      421 cgccgacatc gcgtacgcgc tgatgggcat caacgcggtc aaaggcgtgg aaatcggtgc
      481 cggattcggt gcggtcacgc aactcggcag cgagcacggc gacgaactga cgccggcggg
      541 cttcgtcagc aaccacgccg gcggcatgct gggcggcatc tcgaccggac aggacatcga
      601 ggtgtcgatc gcgatcaagc cgacttcgag catccgcatc gcgcgccgct cgatcaacca
      661 gcgcaacgaa gcggtgtcga tcgaaacaca cgggcgccac gatccgtgcg tcggcatccg
      721 cgccaccccg atcgcagagg cgatgctggc gctggtgctg atcgatcacg cgctgctcga
      781 ccgggcgcag aacgccgacg tcgtgcatgc ggtcgcgccg atcgcgggcg ccgtcgaaaa
      841 gggttgatgt tccgccgcct cccgccggcg gactgggagc cgtggcggcc ggcgctgttc
      901 ttcggagcgt acttcgctgc ggccggcgcg ttcatgccgt acttccccct ttacctcgag
      961 catcgcggca tcgtcgccgg cgagatcggc gtgctgatgg cgaccgcaca ggcgacgcgg
     1021 atcttcgggc cggccttctg gggctggctg gcggaccaca cgttgcagcg gctgcgcatc
     1081 atccgctgga ccgcggtggc gaccgcactt ctgtttgcgt tgctgctggc gccgggcggc
     1141 ttctggtggt tgctgccggt gttcttcgcc acgcagttct gcggcaccgg gcaggtgccg
     1201 gtttgcgagg cgatcatcgc ggcgcgcctg cgcggcgacg cgcacgcacc cgcgcgctat
     1261 gggcggctcc gtgtatgggg atcgatcggc ttcgtcggga cggtgctggc gctggggccg
     1321 gtgctcgatc gcttcggcat cggggtgcag ccgtggctgg tggtgctgct gctggcgctg
     1381 actgcgctgg cgacctttct gctgcgcgac gcaccgcacg ccttcgcgcc gcacgagcgc
     1441 gtgtcggtgc gcgcgcgtct ggccgagccc cgggtgcggt ggttctttgc atcggtcgtg
     1501 ctgatgatct tcgcgcacgg cgcgctgtat tcgtacctgt ccctgcatct ggcgcaactg
     1561 ggttacagca agaccgagat cggcgtgctg tgggtgcttg gcgtggtgat cgagatcggg
     1621 ttcttcgcgt cgcagggccg gttcttccgc cgctacggcg tgtatccgct gctgaccgcg
     1681 acgttcgtgc tggcggcgat ccgcttcgca ctgatcgcgc agtttgcgca cgtggtggtg
     1741 ctgctggtga tcgcgcaact gctgcacgcc acgacctttg ccgtgcatca cagcgcgtcg
     1801 atcctgaccg tgcagcgctg gttcaccggc gcgaccgccg cgcgcgggca ggccttgtac
     1861 gtcagtttgg gatatgggat cggcggcacc gctggcagtc tcggcgctgc ctggctttgg
     1921 tcttccatcg ggccgtcgtc cgcattcttt gccagcagtg tggccgcgct gctgggctgg
     1981 ggagcggtgc gacgctcgca tcagcttgac ttgcaggcag cataggagga gatcgttgga
     2041 atgagacgga tgtttcggcg gcgggtggcg tggatcgcat ttggcgtcgc tgcagttgtc
     2101 gcgggctgcg agaccgtgca gaccacgcgc ggcggcacgg tgggcgttac tcgtgagcag
     2161 cagatgtcgc cgctggtatc gcgcgctcag gtcgacactg ccgcgaagca gcagtacgta
     2221 tcggtgctga aggaatcggg gcagaagggg cagctgaacc gcgactccac gcagacggag
     2281 cgcgtgcgcc ggatcgcgca gcggctgatt ccgcagacgg ccgtgttccg cgacgacgcg
     2341 cccgcctggc agtgggaagt gaacgtgatt acgtccgacc aggtgaatgc gtggtgcatg
     2401 ccgggcggga agatcgccgt ctacaccggc ctgatcgaca agctgaaggt cagcgacgac
     2461 gagctcgcgg ccgtggtcgg ccacgagatc gcacacgcgc tgcgcgaaca cgcgcgcgag
     2521 cgcatggggc ggcagatggc caccgaagcg acgaccgcga tcggcgcagc agcgctggag
     2581 atcttcaccg gcgtgcgcat cgactcgcag gttgcaggca
//
LOCUS       DBNHBY010000227         1673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_960579, whole genome shotgun sequence.
ACCESSION   DBNHBY010000227 DBNHBY010000000
VERSION     DBNHBY010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_960579"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16HB_05670"
     CDS             <1..246
                     /locus_tag="AC16HB_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5606315.1"
                     /translation="HQCFLAPNQLLANLYRVRHLPVSIVQGRYDMVCPIVSADDLARA
                     WPGARYVVVNDAGHSVWEPAIRSAVMREVELFKQALG"
     gene            386..838
                     /locus_tag="AC16HB_05675"
     CDS             386..838
                     /locus_tag="AC16HB_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5606316.1"
                     /translation="MKVSDILRVKGSALFTVTPDESVWTAVQTMADNDIGSVVVMEHG
                     ELVGMLTFREVISTLNVQNGAVGGTLVRRVMDDSPLTITPETDVNEVRRLMLERHARY
                     LPVLDGRALLGVISFYDVAKAVLEEQSFENRMLKAYIRDWPAEESVNR"
     gene            838..>1673
                     /locus_tag="AC16HB_05680"
     CDS             838..>1673
                     /locus_tag="AC16HB_05680"
                     /EC_number="1.6.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606317.1"
                     /translation="MWSHHARVAIVGAGVAGLACARELAAHGAQVAVFDASDTPGGRL
                     ATMVSEAGAYDHGTQYFTVHDERFGAAVRSWEAEGVVQRWNGRLISFTSAGAEDKSRA
                     VERFVAVPGMLRLGMHLARGLDVRYLSTVSRVWRGDAGWHVDCAGQPETADPVAYDVV
                     CVAMPSTQAAAMLRDLTPLAEIAATVNWDPCWAAMMALPRPSGADYDAAFFNDDPILG
                     WCARDSAKPRRGTVTGIAERWVLHARPRWSRRFFQMDEIEVAKWLIRSFSARIRRPLS
                     PT"
BASE COUNT          252 a          542 c          602 g          277 t
ORIGIN      
        1 catcagtgct ttcttgcccc caaccagctg ctcgccaatc tgtaccgcgt gcgccatctg
       61 ccggtgtcga tcgtccaggg ccgctacgac atggtttgcc cgatcgtcag cgccgacgac
      121 ctcgcgcgag catggcccgg ggcacgctac gtcgtcgtca acgacgccgg ccactcggtc
      181 tgggagccgg caatccgctc ggcggtgatg cgcgaagtgg aactgttcaa gcaggcgctc
      241 gggtgaggcg gccgcttgtg agggagtcgg gcactgggct agcatggttt cttgagaaga
      301 cgtaccttgc tcagcctcga ttgctctcct ggccggagag gcgtcgaggt gagggcgggc
      361 aaatcactgg aaccggggcg gcgccatgaa agtcagcgac atcctgcgcg tcaagggcag
      421 cgcactgttc accgtcacgc cggacgagtc ggtgtggacg gcggtgcaga cgatggccga
      481 caacgacatc ggctccgtcg tcgtgatgga gcacggtgag ctggtcggca tgctgacgtt
      541 ccgcgaggtg atcagcaccc tgaacgtgca gaacggcgcg gtcggcggca cgctggtgcg
      601 gcgggtcatg gacgactcgc cgctgaccat cacgcccgaa accgacgtca acgaggtgcg
      661 ccgcctgatg ctggagcgcc acgcgcgcta tctgcctgtg ctcgacggcc gagcgctgct
      721 cggggtgatc tcgttttacg acgttgccaa ggctgtcctc gaagagcaga gcttcgagaa
      781 ccgcatgctg aaggcctaca tccgcgactg gcctgcggag gagtcggtca accggtgatg
      841 tggagccatc acgcccgcgt tgcgatcgtt ggcgcgggtg tggccgggtt agcgtgcgcg
      901 cgcgaactgg cggcacacgg cgcgcaggtc gccgtgttcg acgccagcga cacgccgggc
      961 ggtcgcctcg ccacgatggt ttccgaggca ggcgcctacg accacggcac gcagtacttc
     1021 acggtgcacg acgagcgttt cggggctgcc gtgcgcagct gggaggccga gggcgtcgtg
     1081 cagcgctgga acggacggct gatttcgttc accagcgcag gcgccgagga caagtcgcgc
     1141 gcggtcgagc gctttgtcgc ggtgccgggc atgctccggc tcggcatgca cctcgcgcgc
     1201 gggctcgatg tgcgatacct gagcaccgtg tcccgcgtgt ggcgcggtga cgcgggctgg
     1261 catgtcgatt gcgcgggtca gcccgagacg gcggacccgg tggcgtacga cgtcgtgtgc
     1321 gttgccatgc cctccacgca ggcggcagcg atgttgcgcg acctgacgcc gctggcggag
     1381 atcgccgcga ccgtgaactg ggacccgtgc tgggcggcca tgatggcgtt gccgcgcccg
     1441 agcggcgccg actacgacgc cgccttcttc aacgacgatc cgatcctggg ttggtgcgcg
     1501 cgcgacagcg ccaagccgcg ccgcggcacc gtgaccggca ttgcggaacg ctgggtgctg
     1561 cacgcgcgcc cgcgctggtc gcgccgcttc ttccagatgg acgagatcga ggtggccaag
     1621 tggctgattc gctcgttctc cgcacgcatc cgccggccgc tgagccccac gca
//
LOCUS       DBNHBY010000228         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_554094, whole genome shotgun sequence.
ACCESSION   DBNHBY010000228 DBNHBY010000000
VERSION     DBNHBY010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_554094"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="AC16HB_05685"
     CDS             <1..999
                     /locus_tag="AC16HB_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5606318.1"
                     /translation="KNLKRYNPINISGYHISEAGSSPLQEAAFTLANLIVYVEEVLKT
                     GMKVDDFAPRLAFFFVSQGDFFEEIAKFRALRRCYAKIMKERFGARNPDSMRLRFHCQ
                     TAAATLTKPQYKVNIVRTAMQALSAVLGGAQSLHTNGYDEAFAIPTEDAMKMALRTQQ
                     IIAEETNVTNVIDPLGGSYYIEALTTDYEQRIFQILQEVEERGGTIKLIEQGWFQKHI
                     ADFAYETALRKQSGEKPVIGVNRFVEEEEQHDIEIHPYDPTTAERQIARTQRVRRERD
                     NAKLGRLLDQLVAVAKDPAQNIMPITIEAVKCGATMGDIVEKLKGLWGTYRETPVY"
     gene            1305..>2007
                     /locus_tag="AC16HB_05690"
     CDS             1305..>2007
                     /locus_tag="AC16HB_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR5606319.1"
                     /translation="MNLLSYGGVAPTIVGPPVQFARTAVVIGRVTLGAGAWLDDHAVI
                     RADGHYVRAGTELMLGTRATVHIAHDVYPTLIGDRVTVGEYGVVHACTIGDDCVIEDG
                     AVVLDGCVLEGGSVLAPHSVVFPRTTLPGGFLYSGRPAKPERRLEAGELGLRRERLRE
                     HIRTVDNPVLTGTAKCDFDADVFIANTATLRGHIRAAAQAQVYYACDLDAGSGEIVLG
                     ARCNVQDNSLLHAGTG"
BASE COUNT          374 a          668 c          647 g          318 t
ORIGIN      
        1 aagaacctga aacgctacaa cccgatcaac atctccggct atcacatctc ggaggccggc
       61 tcgtcgccgc tgcaggaagc cgctttcacg ctggcgaacc tgatcgtcta cgtcgaggag
      121 gtgctgaaga ccggcatgaa ggtcgacgac ttcgcgccac ggctcgcgtt cttcttcgtg
      181 tcgcagggcg acttcttcga ggaaatcgcc aagttccgcg cgctgcgccg ctgctacgcg
      241 aagatcatga aggagcgctt cggcgcccgg aatcccgact cgatgcggct gcgcttccac
      301 tgccagaccg cggcggccac actcacgaag ccgcagtaca aggtcaacat cgtgcgcacc
      361 gcgatgcagg cgctgtcggc ggtgcttggc ggcgcgcagt cgctgcacac caacggctac
      421 gacgaggcgt ttgcgatccc gaccgaggat gcgatgaaga tggcgctgcg aacgcagcag
      481 atcatcgccg aggagaccaa cgtcaccaac gtgatcgatc cgctcggcgg cagctactac
      541 atcgaggcac tgaccacgga ctacgagcag cgcatcttcc agatcctgca ggaagtcgag
      601 gaacgcggcg gcacgatcaa gctgatcgag cagggctggt tccagaagca catcgccgat
      661 ttcgcctacg aaacggcgct gcgcaagcaa tcgggcgaga agccggtgat cggcgtcaac
      721 cgtttcgtcg aggaggagga gcagcacgac atcgagatcc atccctacga tccgaccacc
      781 gcggagcgcc agatcgcccg cacgcaacgc gtacggcgcg agcgggacaa cgcgaagctc
      841 gggcgcctgc tcgaccagtt ggtcgcggtg gcgaaggacc ccgcgcagaa catcatgccg
      901 atcacgatcg aggcggtgaa gtgcggtgcg acgatgggcg acatcgtcga gaagctgaag
      961 gggctgtggg ggacttatcg ggagaccccg gtctactgac acacgcttcc cgttcatttc
     1021 ctgcggcttc gaggcgaccc ttcaacttcg ctgcggtgga cgaaaaagga gctcgctcgg
     1081 gcccctcgtg ctcacccagc aggatcgagg caagcgactt cgagcctgat attgctcccg
     1141 ggacactcca aaaacaccaa gtgacgtcag gcccgccaac actcacaccc cactccaccg
     1201 caggcgtgat cgcgcgcttc ctgaaggcgc gcgcgttcgg gttgttcggc gagcgtgtcg
     1261 agcgcgcctg gcgcggtcgc tagaatcccg ccatgagcac gcaaatgaac ttgctttcct
     1321 acggcggcgt ggcacccacg atcgtcggtc cgccggtgca gttcgcccgc accgcggtcg
     1381 tgatcggacg cgtcacgctc ggggccggcg cctggctcga cgaccacgcg gtgatccgcg
     1441 ccgacggcca ctatgtgcgc gcgggcaccg agctgatgct gggaacacgt gcgacggtcc
     1501 atatcgcgca tgacgtctac ccgacgctga tcggcgaccg ggtcaccgtc ggtgagtacg
     1561 gcgtcgtgca cgcctgcacg atcggcgacg attgcgtgat cgaagacggc gcggtcgtgc
     1621 tcgacggttg cgttctcgaa ggcggcagcg ttctggcgcc ccacagcgtc gtgtttcccc
     1681 gcaccacgct gccgggcggt ttcctgtatt cgggccgtcc ggcgaagccc gagcgtcggc
     1741 tcgaagctgg cgagctcggt ctgcgccgtg aacggctgcg cgagcacatc cgcacggtcg
     1801 acaatcccgt gctgaccggc accgcgaaat gcgacttcga tgccgacgtg ttcatcgcca
     1861 ataccgccac gctgcgcggc catatccgcg ccgccgcgca ggcgcaggtc tactacgcgt
     1921 gcgacctcga cgccggcagc ggcgagatcg tgctcggtgc gcgctgcaac gtgcaggaca
     1981 attcgttgct gcacgcgggc acgggcc
//
LOCUS       DBNHBY010000229         4582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_960618, whole genome shotgun sequence.
ACCESSION   DBNHBY010000229 DBNHBY010000000
VERSION     DBNHBY010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_960618"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="pheT"
                     /locus_tag="AC16HB_05695"
     CDS             <1..1755
                     /gene="pheT"
                     /locus_tag="AC16HB_05695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR5606320.1"
                     /translation="WMKQRLERSGQRPISALVDISNYVMLELGRPSHIFDLDKVHGSL
                     TVRWGRKGEQVELLNGQRVEVDEQVGVIADDLRVEALAGVMGGAPTAVSLATRNIYIE
                     AAFWWPESIQGRARRYNFSTDAAHRFERGVDFSTTADHIEIITQLVLSVCGTADTQIG
                     PIDDQVVRLPERRPVRMRTDRCRKVLGVAIGDDEMTQVFRRLGLAFQREPEAILVTPP
                     PYRFDLEIEEDLIEEVARLWGFERIAAHPPRTRAAMRATREDRRSTHALRRELAHAGY
                     QELVNFSFVDAAWESDFAANDAPIRVVNPIAAQYAVMRSSLLGGLVAALTYNLNRKAG
                     RVRVFELGRVFLRDATAQDGPLAVAGTRQPLCVAGIAYGPAVEEQWGERRRDVDFFDV
                     KGDVERLIGNESVRFHKFKHAALHPGRAARIGLEGRAIGWIGELHPRWQQKYELPKAP
                     IAFELDVDALLATRIPVAQPVARTPAVIRDIALIVEERVSVQEIQDVVEELVRSDEHL
                     SSLREFRLFDLYRASAADSSKVAGVGANVLLNKEKNLAFRMVLQDTERTLSDADVDAS
                     VAAVLAGLTARVGARLRQ"
     gene            1760..2137
                     /locus_tag="AC16HB_05700"
     CDS             1760..2137
                     /locus_tag="AC16HB_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR5606321.1"
                     /translation="MQNESDLTAFKYAEVPPAAAQDVERTGHTITKAELADALFERLG
                     LNKREAKDMVDAFFDEIRAALERGESVKLSGFGNFQLRDKPQRPGRNPKTGEEIAITA
                     RRVVTFHPSQKLKAAVSDANDAG"
     gene            2169..2558
                     /locus_tag="AC16HB_05705"
     CDS             2169..2558
                     /locus_tag="AC16HB_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5606322.1"
                     /translation="MQRIDSNHAVGGALPAIPAKRYFTIGEVSELCGVKPHVLRYWEQ
                     EFTQLKPIKRRGNRRYYQHHEVLLVRRIRELLYEQGFTISGARNRLDEGGRGGRNSGR
                     ETGPTAVLGLAEVRAELEAIRALLRRE"
     gene            2588..2664
                     /locus_tag="AC16HB_05710"
     tRNA            2588..2664
                     /locus_tag="AC16HB_05710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2622..2624,aa:Pro,seq:ggg)
     gene            2722..3474
                     /gene="surE"
                     /locus_tag="AC16HB_05715"
     CDS             2722..3474
                     /gene="surE"
                     /locus_tag="AC16HB_05715"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5606323.1"
                     /translation="MRILVSNDDGYLAPGLAALVEAVRPFGEVFVVAPEQNHSGASNS
                     LTLSRPLSVQRAANGFFYVNGTPTDCVHIALTGLLEHRPDLVVSGINNGQNMGDDTIY
                     SGTVAGAMEGYLFGVPSIAFSLIDKGYEHLDSARAIATEIVTHWCANPLPAPSLLNVN
                     IPNLPRERMRGIRCTRLGKRHPSEAVIRAQNPYGDTIYWVGPAGSARDAGPDTDFYAT
                     AQGYVSVTPLQIDLTHTEQLPQVAAWLQRGHN"
     gene            3450..4160
                     /locus_tag="AC16HB_05720"
     CDS             3450..4160
                     /locus_tag="AC16HB_05720"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5606324.1"
                     /translation="MASARSQLKPPVTLSPNTGLASERVRARMVERLRAQGVTDARVL
                     AAMEWVPRHQFVDAALASRAYEDTALPIGGGQTISRPHIVARIAELALEGQQASKTAK
                     VLEIGTGCGYACAVLARLFGTVISVERVRLLHELARRNLRPLRLPNVRLVFGDGNDGI
                     AAEAPFDAIVAAAAGERIPEAWIEQLKPGGRIVAPVITNPADDAQALKVVTKDAAGRL
                     ASKAVQAARFVPLRFGTK"
     gene            4178..>4582
                     /locus_tag="AC16HB_05725"
     CDS             4178..>4582
                     /locus_tag="AC16HB_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5606325.1"
                     /translation="MMGKPIRKPISVLLLAGALLQAACTTTGVRQAPIVERAPPTAGA
                     TPEVPPTPPVKREARDGIYVVQKCDTLRQIGAAFGHDPRDIARWNNIEDPNTLRPGQD
                     LRVVPPADAAVATPVTPATGAQSRPLTTTVPGV"
BASE COUNT          794 a         1448 c         1595 g          745 t
ORIGIN      
        1 tggatgaagc agcgcctgga gcgcagcggg cagcgtccga tttcggcgtt ggtcgacatc
       61 tccaactacg tgatgctcga gctcgggcgg ccttcgcaca ttttcgacct cgacaaggtg
      121 cacgggagtc tcaccgtgcg gtgggggcgc aagggcgagc aggtcgagtt gctgaacggc
      181 cagagggtcg aggtcgacga gcaggtcggc gtgatcgccg acgacctccg cgtcgaggcg
      241 ctggctggcg tcatgggcgg tgcacccacg gcggtgtccc tcgcgacccg caacatctac
      301 atcgaagcgg cgttctggtg gccggagtcg atccagggtc gcgcgcgtcg ctacaacttc
      361 tcgaccgacg cggcgcaccg cttcgagcgt ggcgtcgact tctcgaccac cgccgatcac
      421 atcgagatca tcacgcaact cgtgctgtcg gtctgcggca ccgcggacac gcagatcggc
      481 ccgatcgacg accaggtcgt gcgcctgccg gaacgcaggc cggtgcggat gcgcacggat
      541 cgctgccgca aggtgctcgg cgtggcgatc ggcgatgacg agatgacaca ggtcttccgg
      601 cggctcggac tcgcgtttca gcgcgagccc gaagcgatat tggtcacccc gccgccgtat
      661 cgcttcgatc tggagatcga ggaagacctg atcgaggaag tcgcgcggct gtggggattc
      721 gagcgcatcg ccgcgcaccc gccacgcacg cgcgcggcga tgcgcgccac gcgcgaggat
      781 cggcgctcga cgcacgcgct gcggcgcgag ctggcgcacg ccggctatca ggagttggtg
      841 aatttctcgt tcgtcgatgc ggcgtgggag agcgatttcg ccgcgaacga tgcgccgatc
      901 agggtcgtga atccgatcgc cgcgcagtac gccgtgatgc gttcgtcgct gctcggcgga
      961 ttggtcgctg cgctcacgta caacctgaat cgcaaggccg ggcgggtgcg cgtgtttgag
     1021 ctcggacgcg tgttcctgcg cgacgccacg gcgcaagatg gtccgctcgc ggtcgcgggt
     1081 acacggcagc cgctgtgcgt ggcggggatt gcgtatggtc cggcggtcga agagcaatgg
     1141 ggcgagcgca ggcgcgacgt ggatttcttc gacgtgaagg gcgacgtcga gcggctgatc
     1201 ggcaacgagt cggttcggtt ccacaaattc aagcacgcag cgctgcatcc gggccgcgcc
     1261 gcgcgcatcg ggctcgaggg gcgtgcgatc ggctggatcg gcgaactgca tccgcgctgg
     1321 cagcagaagt acgaactgcc gaaggcgccg atcgcgttcg agctcgatgt ggacgcgttg
     1381 ctcgccaccc gcatcccggt cgcgcagccc gtggcgcgga cgcctgccgt gattcgggac
     1441 attgcgctga tcgtcgagga gcgcgtgagc gtgcaggaga tccaggatgt cgtggaggag
     1501 ctcgtccgtt cggacgagca cctttcatcg ctgcgcgaat tccgcctgtt cgacctgtac
     1561 cgcgcatccg ccgcagattc aagcaaagtc gcgggagtag gcgctaacgt gttgttaaat
     1621 aaagagaaaa atcttgcgtt cagaatggtt ctgcaagata ctgaacgcac tctcagcgat
     1681 gcggacgtgg atgcctcggt tgctgccgtg cttgcagggt tgacggcccg agtcggtgcc
     1741 aggctccgac aatgaatcga tgcagaacga atcggatctg accgcgttca agtacgccga
     1801 ggtgccgccc gccgcggcac aggatgtcga acgcacgggt cacacgatca ccaaggcaga
     1861 actggcggat gcactattcg agcggctcgg cctgaacaag cgcgaagcca aggacatggt
     1921 cgacgcgttc ttcgatgaaa tccgtgcggc actcgagcgc ggcgaatcgg tgaagttgtc
     1981 cggcttcggc aacttccagc tgcgcgacaa gccgcagcgg ccgggacgca acccgaagac
     2041 cggcgaagag atcgcgatca ccgcacggcg cgtggtcacg tttcatccga gccagaaact
     2101 gaaggccgcg gtctccgacg cgaacgacgc cggataggtc gggcggtctg gcactgaagc
     2161 gatcatcgat gcagcggatc gactccaacc atgcggtggg cggcgcactg cccgcgattc
     2221 cggcgaaacg ctacttcacg atcggcgaag tgagcgaact gtgcggcgtc aagccgcacg
     2281 tgctgcgcta ctgggaacag gagttcaccc agttgaagcc gatcaagcgg cgcggcaacc
     2341 gccgctatta ccagcaccac gaggtcctgc tcgtgcgccg cattcgcgag ttgctgtacg
     2401 agcaggggtt cacgatcagc ggcgcgcgca atcgcctcga cgagggcggc cgcggcgggc
     2461 gcaacagtgg ccgcgaaacc gggccgacgg cggtgctggg ccttgccgaa gtgcgcgcag
     2521 agctggaagc gatacgagcg ctgttgcgtc gtgagtgacg aatcgcgcgt gtacaatgcc
     2581 gcgcgatcgg ggcgtagcgc agcctggtag cgcacttgca tggggtgcaa ggggtcgcga
     2641 gttcgaatcc cgccgccccg accaggacac ccggagaagg ccggtgcgcg cggagcgtgc
     2701 accggccact tcgtttctgt gatgcggatt ctcgtttcca acgacgacgg ttatctcgct
     2761 ccgggcctgg cggcgctggt cgaggctgtg cgaccgttcg gcgaggtgtt cgtcgtcgca
     2821 cccgagcaga accacagcgg cgccagcaat tcgctgacgc tgtcacggcc gctgtcggta
     2881 cagcgtgcgg ccaacggctt cttctacgtg aacggcacgc ccaccgattg cgtgcacatc
     2941 gcgcttacgg gcctgctcga gcaccggccg gacctcgtcg tctccggtat caacaacggc
     3001 cagaacatgg gcgacgacac gatctactcc ggcacggtcg ccggagcgat ggagggatac
     3061 ctcttcggtg taccgtcgat cgcgttctcg ctgatcgaca agggctacga gcatctggac
     3121 agcgcgcgcg cgatcgcgac cgagatcgtc acgcactggt gcgcgaatcc gctgccggcg
     3181 ccttcactgc tgaacgtcaa cattccgaat ctgccgcgtg aaaggatgcg cggcatccgc
     3241 tgcacgcggc tcggcaagcg tcatccttcg gaggcggtga tccgggccca gaacccgtac
     3301 ggcgacacga tctactgggt gggccctgcc ggcagcgcgc gcgatgcggg tcccgacacc
     3361 gatttctatg ctacggcgca aggctatgtg tcggtgacgc cactgcagat cgatctgacc
     3421 cacaccgaac agctgcctca ggtcgcggca tggcttcagc gcggtcacaa ctgaagccgc
     3481 cggtcacgct gtcgcccaac acgggtcttg cctctgaacg cgttcgcgcg cgcatggtcg
     3541 agcggctgcg cgcgcagggc gtgaccgacg cgcgcgtgct cgcggcgatg gaatgggtcc
     3601 cgcgtcatca gttcgtagac gcggcattgg cgagccgcgc ctacgaggac actgcgttgc
     3661 cgatcggcgg cggccagacc atctcgcggc cgcacatcgt ggctcggatc gcggagctcg
     3721 cgcttgaggg gcagcaggcg tcgaagaccg caaaggtgct ggagatcggc accggttgtg
     3781 gctacgcctg tgcggtgctg gcgcgtctgt tcggcaccgt gatctcggtc gagcgcgtgc
     3841 ggctgctgca cgaactggca cgacggaacc tcaggccgtt gcgactgccg aacgtgcggc
     3901 tggtgttcgg cgacggtaac gacggcattg cagccgaagc acctttcgac gcgatcgtcg
     3961 ccgcggctgc gggcgagcga attcctgagg cgtggatcga gcagctgaag cccggcggcc
     4021 gcatcgttgc gcccgtcatc acgaatccgg cggacgacgc gcaggcgctg aaagtcgtga
     4081 cgaaggacgc ggcggggcgg ttggcgtcga aggccgtgca ggcggcacgc tttgtaccgt
     4141 tacgattcgg gacgaaatga tcaggatgac gatacggatg atgggcaagc cgatccgaaa
     4201 gccgatttcg gtcctgttgc tggccggtgc attgctgcag gcggcctgca cgacgacggg
     4261 tgtgcgccag gcgcctatcg tggagcgcgc tcctccgacc gcgggcgcga cgccagaagt
     4321 ccctccgacg ccgcccgtta agcgcgaggc gcgcgacggc atttacgtgg tgcagaagtg
     4381 cgacacgctg cggcagatcg gagccgcatt cggccatgac ccgcgcgaca tcgcgcgctg
     4441 gaacaacatc gaggacccga acacgctgcg tcccggacag gatctacgcg tcgtgccgcc
     4501 ggccgacgcc gccgtggcca caccggtcac gcccgccacc ggcgcgcagt cgcggccgct
     4561 gacgacgacc gttcccggcg tc
//
LOCUS       DBNHBY010000230         6370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1637075, whole genome shotgun sequence.
ACCESSION   DBNHBY010000230 DBNHBY010000000
VERSION     DBNHBY010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1637075"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="AC16HB_05730"
     CDS             <1..158
                     /locus_tag="AC16HB_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5606326.1"
                     /translation="QVWDMNFDSDTNVVEVAVKRLRAKVDDPFEHKLIHTIRGVGYVL
                     EDRSPQW"
     gene            155..1588
                     /locus_tag="AC16HB_05735"
     CDS             155..1588
                     /locus_tag="AC16HB_05735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HIR5606327.1"
                     /translation="MTQRRLGGDSITARLALLFAVALAALSLAAGGYLYHALTIDLTR
                     RDEDELHGKMALAVEAVGRIDSFADVSQRDSELAHVVVGHERLTLYVVGADDRMLFAT
                     GHPRLDAGARAALLESARPQMFATGSHSVRALGSRAALRSATPVGVFIESDTAETDRL
                     LAGHLREIVLAVLLSAVLGLGAAYGIARAGLRPLREMADTAKRISADQLDQRLLVEAA
                     PAELRQLASAFNAMLQRLQESFTRLNDFSSDLAHEMRTPINNLIGHTQVVLSRSRSAD
                     DYRAALESNMEEFERLSRMVGDMLFIARADSPQTRVARRRVDLRAAALKMQQFYEPVL
                     ADRGLSLSVTGAAHVEADPLMVERAVSNLVSNAVRHAATGSEIAIRIDNEPPTTTLSV
                     SNRGQPIPPEVKDRLFSRFAHADTGGRNGDGIGLGLAIVQSIMKLHDGAVTVESSAGG
                     ETRFVLSFAASLEAAVESVPAPARPSP"
     gene            complement(2013..2615)
                     /locus_tag="AC16HB_05740"
     CDS             complement(2013..2615)
                     /locus_tag="AC16HB_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606328.1"
                     /translation="MTKSFIHAAPLWLLAVSSIVYATDLYPWRDHSPPFDFRFGNDID
                     AHQQTRRTPDGGLFGFFYVRFTGVVTKDRYRVASHADCSAFTDCTVGWTLNGVPQNAT
                     YLYQLASDHPVFLVARTGVPQPGAHAHFHWLGAMPSVGQSVSGYLLQLTAVDKFCFIH
                     HDAESATTNRTCSDNGGVNVDFGTDIATHMNIVASRPPGL"
     gene            2785..3996
                     /locus_tag="AC16HB_05745"
     CDS             2785..3996
                     /locus_tag="AC16HB_05745"
                     /inference="COORDINATES: protein motif:HMM:NF017000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HIR5606329.1"
                     /translation="MLFDPARHELLRAEAWDEQRAHDAIARIVRDTEARFSAQSHWPI
                     HPKDADGGDVGPLFPLYFGACGTVWALHYLQAAGAARLERSYADYVDSLLPLNQAWLR
                     SIGSADFASYLMGDTGLLLLSHWMYPTEQKAARLEALIAGNLDNPTRELMWGAPGTLL
                     AALFLHQRTGEARWAELYRATARKLWSQLQWSSEFECHYWTQDMYGNTSTYLDGVHGF
                     VATAAPLIHGRHLLAPAEWEDWNQCITRTIARTAMREAGRVNWPALLYMPAGRAPVLL
                     MQFCHGAPGFVVCLADIPGTALDELLTAAGESIWDAGPLIKGSSLCHGTGGNGYAFLK
                     LYQRTRDALWLERARAFAMHAIAQTEADAARYGQMRYSLWTGDPGLAIYLWDCIRGGG
                     AFPTLDVFCAN"
     gene            4169..4543
                     /locus_tag="AC16HB_05750"
     CDS             4169..4543
                     /locus_tag="AC16HB_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606330.1"
                     /translation="MKLARTDRIRTILVVLCALLLAQWSLAAHACPKVWVANPTPVSA
                     AEQDPCCADEGDAPSVLCYKHCNADEQASGGIAVAFAAPPPALVIARSTDVVRIESPA
                     STLGLAHATAPPFTILYCVSLT"
     gene            4627..5097
                     /locus_tag="AC16HB_05755"
     CDS             4627..5097
                     /locus_tag="AC16HB_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR5606331.1"
                     /translation="MNTRISRRNALQTLALLATAPLLARAAPALPTVEVYKSPTCGCC
                     EDWIRHLEASGFKVKATDVPDSRNYRVRFGMPNKYGSCHTAVVGGYVVEGHVPAREIE
                     RLLREKPNALGLAVPGMPIGSPGMDGPEYKGLKDMYDVLLVARDGSARVWASYN"
     gene            5106..>6370
                     /locus_tag="AC16HB_05760"
     CDS             5106..>6370
                     /locus_tag="AC16HB_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5606332.1"
                     /translation="MNASVRLLAAAAALAASAALAQSPAPAELGATLQGLLDYAAARH
                     PELRSMRYEADAAAARVLPAGALPDPMFQIELRDIDNSGASSPNLLPGRVGSTRYQIR
                     QTLPSWGKRDAKRDAAQASADEAAYRTDATWTELAMRIKSTYARYQQTTDGLVQSREI
                     LDLVNRLESVAQVRYAGGLAPQQDALRAQVERTSMTSEIAMLEADLAGTRARINGLLA
                     RPSDAPLAAPRTEAALPPLARVDFATVRDRVLARNPQLKAEDARIRAAEKNKDAVFAN
                     RYPEFTFGVSPIQMRNRIAEWELMFEVNIPLQQSSRRADEREALSMLAAAQARREAQL
                     NDVLAQLGESVAGYEAAHRVETLTRTSLLPQAEVTFQAALAGYETGKVDFTTVLEAQR
                     QIRQARLTLIRTRAEARMKQAEIERLLGE"
BASE COUNT         1079 a         2169 c         2102 g         1020 t
ORIGIN      
        1 agcaggtgtg ggacatgaac ttcgacagcg acaccaacgt ggtcgaagtg gcggtcaagc
       61 ggctgcgcgc caaggtcgac gatccgttcg aacacaagct gatccacacc atccgcggcg
      121 tcggctacgt gctcgaggac cgctcgccgc agtggtgacg cagcggcgcc tgggcggcga
      181 ctcgatcacg gcgcggctcg cgctgctgtt cgcggttgcg ttggccgcac tgtcgctcgc
      241 cgccggcggc tatctgtatc acgcgctgac gatcgatctg accaggcgcg acgaggacga
      301 actgcacggc aagatggcgc tcgcggtcga ggcagtcggc aggatcgact cgttcgccga
      361 cgtgagtcaa cgcgactccg agctcgcgca cgtcgtggtc ggtcacgagc ggctcacgct
      421 gtacgtggtc ggcgccgacg accgcatgct gttcgccact ggccatccgc gactggacgc
      481 gggcgcgcgc gccgcgctgc tcgagtcggc gcggccgcag atgttcgcca ccggcagcca
      541 ctcggtgcgt gcgctcggca gccgcgccgc gctgcgatcg gcaacgccag tcggcgtctt
      601 catcgagtcc gacaccgcgg aaaccgaccg cctgcttgcc ggccatctgc gcgagatcgt
      661 cctcgccgtg cttctgagcg ccgtgctcgg actcggcgcc gcatacggca tcgcccgcgc
      721 cgggctgcga ccgctgcgcg agatggccga caccgccaag cgcatctcgg ccgatcagct
      781 cgaccagcgg ctgttggtcg aagctgcgcc ggccgagctg cgacaactcg cgagcgcgtt
      841 caacgcgatg ctgcagcggc tgcaggagtc cttcacccgg ctgaacgact tctcgtcgga
      901 tctggctcac gagatgcgca cgccgatcaa caacctgatc ggccacacgc aggtcgtgct
      961 gtcgcgcagc cgcagcgccg acgactaccg cgcggcgctc gaatcgaaca tggaagagtt
     1021 cgagcgtctg tcgcgcatgg tcggcgacat gctcttcatt gcgcgcgccg atagcccgca
     1081 gacgcgcgtg gcgcgacggc gcgtcgacct gcgcgcggcg gcgctcaaaa tgcagcagtt
     1141 ctacgaaccg gtcctcgccg accgcggact ttcgctcagc gtcaccggcg cggcccacgt
     1201 cgaggccgac ccgctgatgg tcgagcgcgc ggtcagcaat ctcgtctcca acgccgtgcg
     1261 ccatgccgcg accggcagcg agatcgcgat tcgcatcgac aatgaaccgc ccacgaccac
     1321 gctttccgtc agcaaccgcg gccagccgat tccgccagag gtaaaggacc gcctgttcag
     1381 ccgctttgcc cacgccgaca cgggtggccg caacggcgac ggcattggcc tcgggcttgc
     1441 gatcgtgcag tcgatcatga agctgcacga cggcgcggtg accgtagaaa gcagcgcggg
     1501 cggcgagacg cggtttgtgc tgtcatttgc cgcgtcgctc gaagccgcgg ttgaatcagt
     1561 tccggccccc gccagaccat cgccttgaat cgcaaccgaa gtccctcgga gcgctattcg
     1621 cctacggtgt gcgctcgaca aactgctgct cgaggcaagc gaggcgaact ggcagtcagt
     1681 taccctctcg aagggctcga acccgtgcca cggcactggc ggcaatggct acggttgctg
     1741 aagctctact tgcgcacgcg cgacccgctg tggcgcgatc gcggggttcc cctcgggccg
     1801 ccgattgcag aacgatgccg gcaaggttca cggcgaagtc gcgccccgcg agtcaatgca
     1861 gaacctgcgt gagggcgctc ccgtttgccc gctgcacgga ttgcggaaga actccctcaa
     1921 cgagttgaag cgacttgctc cggcgtgcag tactgcagca tgctgcgcgg gaaccggtct
     1981 ttacaaaggg cgccaagatc tgcgcgccat cgctacagtc cgggcggtcg gctcgcgacg
     2041 atgttcatgt gagtggcgat gtcagtcccg aaatcgacgt tcacgccccc attgtcactg
     2101 caggtccggt ttgtcgtcgc gctctcggcg tcgtgatgga tgaagcagaa tttgtcgacc
     2161 gcagtcaact gcagcaggta tcccgacact gactgcccga ccgatggcat cgcgcccagc
     2221 cagtgaaaat gcgcatgggc gcccggctgt ggaacgcccg tccgggcgac caaaaacacc
     2281 gggtgatcgg acgcaagttg atacagatag gttgcattct gcggcactcc gttcagggtc
     2341 cagcccacgg tacagtccgt gaacgcgctg caatcggcgt gcgacgcaac gcggtaccga
     2401 tcctttgtca caacgccggt gaaacgcacg tagaagaatc cgaacagccc gccgtcgggc
     2461 gtccttctcg tttgctggtg cgcatcgatg tcgttgccga aacgaaaatc aaacggcggc
     2521 gagtgatcgc gccaggggta cagatcggtg gcgtagacga tactgctcac cgccagcagc
     2581 catagcggtg cggcatggat gaatgacttg gtcatgcgaa cacctcgtaa aagacagggg
     2641 catggccgtc gtcggacggc gtttctcgac tatccgacgt cgctcaacca aaggacagcg
     2701 ccgataccgc ctttcggctg caagtgtgac ctgtgctcgg gatcgttcgc gcatccggcg
     2761 ccacgaacca ccgagtagcc gtcgatgcta ttcgaccccg cacgccacga actcctgcgg
     2821 gctgaagcct gggacgagca gcgcgcgcac gacgcgatcg cgcgcatcgt gcgcgatacg
     2881 gaggcccgct tctccgcgca gtcgcactgg ccgattcacc ccaaggatgc cgatggcggt
     2941 gacgtcgggc cgctttttcc gctctacttc ggcgcctgcg gcaccgtctg ggcgctgcat
     3001 tatctgcaag ccgccggcgc ggcgcgcctc gagcgatcct atgcggacta tgtcgattcg
     3061 ctgctgccgc tgaaccaggc gtggctgcgt tcgatcggca gcgcggattt cgcgtcctac
     3121 ctgatgggcg acaccggtct tctgctgctc agccattgga tgtatccgac ggagcagaag
     3181 gccgctcgtc tcgaagcgct gatcgcgggc aatctcgaca acccgacgcg cgaactgatg
     3241 tggggtgcgc ccggcacgct gctcgccgcg ctgttcctgc atcagcgcac cggcgaagcg
     3301 cgctgggcgg agttgtatcg cgccacggcg cgcaagctgt ggtcgcaact gcagtggtcg
     3361 tccgaattcg agtgccatta ctggacgcag gacatgtacg gcaacacgag cacgtatctc
     3421 gacggcgtgc acggcttcgt cgccaccgcg gcaccgctga tccatggccg gcacctgctg
     3481 gcaccggccg agtgggagga ttggaaccag tgcatcacgc ggaccatcgc gcgcacggcg
     3541 atgcgcgaag caggccgcgt caactggccc gcgctgctgt acatgccggc aggccgcgcg
     3601 ccggtcctgt tgatgcagtt ctgccatggt gcgccgggct tcgtcgtctg cctcgccgat
     3661 attccgggca cggcactcga cgaactgttg actgccgccg gcgaatccat ctgggacgcc
     3721 ggcccgctga tcaaggggtc gagtctgtgc cacggcaccg gcggcaacgg ctatgcgttt
     3781 ctcaagctgt atcagcggac acgcgatgcg ctgtggctcg aacgcgcgcg ggcgttcgcg
     3841 atgcacgcga ttgcgcagac cgaggccgac gcagcgcgtt acggacagat gcgctattcg
     3901 ctgtggactg gcgatccggg gctcgcgatc tacctatggg actgcatccg cggcggcggc
     3961 gcttttccga cgctcgacgt gttctgtgcc aactgacgtt gctccaggaa acggggacat
     4021 gtcgcaagtt caccgcgctc cgcttcattc ggtgccgcag cggaacgctt cgcgcgaccg
     4081 cgcgacgtgc ctggcgtttg cgctagcaca aacatacgtg acggaccgga cgtgtaacct
     4141 acgccgtaac gatgccgctc tgcagccgat gaaactcgcc cgaaccgacc gcattcgcac
     4201 gatcctggtc gtgctgtgcg cgctgctgct ggcgcaatgg tcgttggccg cccacgcctg
     4261 tccgaaggtg tgggtcgcca accccacgcc cgtctcggca gcggagcagg atccgtgctg
     4321 cgcggacgaa ggcgacgccc cgtccgtcct ctgctacaag cactgcaacg ccgacgagca
     4381 ggcgtcgggc ggcatcgcag tcgcgttcgc cgcaccacca cccgcactcg tcatcgcgcg
     4441 ttccaccgat gtggtgcgca tcgagtcgcc cgcatcgacc ctcgggctgg cgcacgcgac
     4501 cgccccgccc ttcacgatcc tgtactgcgt ttccctgacc tgacgtcgat cgccgcgggc
     4561 cgcgcgcccg cgtgatttcg atcgagttca ctttcgcgac ccgctgcggt cgctcaggag
     4621 aaacgcatga acacccgcat ttcacgccgt aacgcgctgc agacgctggc gctgctggcc
     4681 acggccccac tgctcgcccg cgccgcgccc gccttgccga cggtcgaggt ctacaagagc
     4741 ccgacgtgcg gctgctgcga agactggatc cgacacctcg aagccagcgg cttcaaggtg
     4801 aaagccactg acgttcccga ctcgcgcaac taccgcgtgc gcttcggcat gccgaacaag
     4861 tacggctcgt gccacaccgc ggtggtgggc ggctacgtgg tcgagggaca tgtgccggca
     4921 cgcgagatcg agcggctgct gcgcgagaag ccgaacgcgc tcgggctggc ggtgccggga
     4981 atgccgatcg gctcgccggg catggacggc cccgagtaca agggtctgaa ggacatgtac
     5041 gacgtgctgc tcgtcgcacg cgatggcagc gcgcgcgtgt gggcgagcta caactaggtg
     5101 cggccgtgaa cgctagcgtc cgtctactgg cggcggctgc cgcgcttgcg gcgagcgccg
     5161 cattggcgca gtcgcccgcg ccggccgaac tcggcgccac gctgcaggga ctgctcgact
     5221 acgcggccgc gcgccacccg gaactgcgct cgatgcgcta cgaggccgac gccgccgcgg
     5281 cgcgcgtcct ccccgccggc gcgctgcccg acccgatgtt ccagatcgag ctgcgcgaca
     5341 tcgacaacag cggcgcgtcg agcccgaacc tgctgccggg gcgcgtcggc agcacgcgct
     5401 accagattcg acagactttg ccgtcgtggg gcaagcgcga tgcgaagcgc gacgcggcac
     5461 aggcgtccgc cgacgaagcc gcataccgga ccgacgcgac gtggacggag ctggcgatgc
     5521 ggatcaagtc gacttatgcg cgctaccagc agacgaccga cggtctcgtg cagtcgcgtg
     5581 agatcctcga cctcgtgaac cggctcgagt cggtcgcgca ggtgcgctac gcgggcggcc
     5641 tcgcgccgca gcaggatgcg ctgcgcgcgc aggtcgagcg cacctcgatg acgagcgaga
     5701 tcgccatgct tgaggcggat ctcgccggca cgcgcgcacg catcaacggt ctgctggcgc
     5761 gcccctccga cgcgccgctg gcggcaccgc gcacggaagc cgcactgccg ccacttgcac
     5821 gcgtcgattt cgcgaccgtg cgcgatcgcg tgctggcacg caacccgcaa ctgaaggccg
     5881 aggatgcgcg catccgtgcc gccgaaaaga acaaggacgc ggtgtttgcc aaccgctatc
     5941 cggagttcac gttcggagtc tcgccgatcc agatgcgcaa tcgcatcgcc gaatgggagt
     6001 tgatgttcga ggtcaacatc ccgctgcagc agtcgagtcg ccgcgccgac gagcgcgaag
     6061 cgctttcgat gctcgcggcc gcgcaggcgc ggcgcgaggc gcagttgaac gacgtgctgg
     6121 cgcaactcgg cgagagcgtg gccgggtacg aagccgcgca ccgcgtcgag acgttgacgc
     6181 gcaccagcct gttgccacag gcggaagtga catttcaggc ggcactcgcc ggctacgaga
     6241 ccggcaaggt cgatttcacg acggtgctcg aagcgcagcg gcagatccgc caggcgcgac
     6301 tcacgctgat ccgcacgcgc gcggaagcac gcatgaagca ggccgagatc gagcggctgc
     6361 tgggagagga
//
LOCUS       DBNHBY010000231        10468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1840303, whole genome shotgun sequence.
ACCESSION   DBNHBY010000231 DBNHBY010000000
VERSION     DBNHBY010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1840303"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1112
                     /gene="prfA"
                     /locus_tag="AC16HB_05765"
     CDS             30..1112
                     /gene="prfA"
                     /locus_tag="AC16HB_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5606333.1"
                     /translation="MKPSMQAKLAHLARRLNEIDTALASEDATRDMNRYRALGQERAE
                     IEPVVARFHDYNRAQADLAGAQEMLAEPDMKALAEEEIHVARGRIAALEDDLQRLLLP
                     RDPNDERNVFLEIRAGTGGDESALFAGDLLRMYSRFAERHRWQTELISESPSDLGGYK
                     EVIVRIIGAGAYSKLKFESGGHRVQRVPETEAQGRIHTSACTVAVLPEADEINEVKID
                     PSELRIDVFRASGAGGQHVNKTESAVRLTHLPTGIVVECQDDRSQHKNRERAMKILAA
                     RIKDKQVQEQRSKEASTRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDQIMD
                     GDLDEIAAALTAEHQAELLAALGETA"
     gene            1109..2503
                     /gene="fumC"
                     /locus_tag="AC16HB_05770"
     CDS             1109..2503
                     /gene="fumC"
                     /locus_tag="AC16HB_05770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR5606334.1"
                     /translation="MTTRTEQDTFGPIEVPAERLWGAQTQRSLQYFAVSTEKMPPELI
                     RALVLIKRAAAGVNAELGLLEARKGDAIVAACDEVLAGRHDGEFPLSVWQTGSGTQTN
                     MNVNEVLANRASELLGGARGPGRLVHPNDDVNRCQSSNDVFPTAMHVAAALALDRQLR
                     PALCGLRTTLDDKARAFADIVKIGRTHLQDATPLTFGQEISGWAAQLEAAGSAIVAAE
                     PALLRLAIGGTAVGTGLNAHPEFGARVAARLAADTGLAFAPAPNKFAALAGHEPLLAA
                     HGALKTLAAALMKIANDVRWLASGPRAGIGELTIPENEPGSSIMPGKVNPTQCEMLTM
                     VAAQVLGNDVAIGIGAASGNFELNVFKPLLAHAFLQSVRLLADGSASFDAHCARGIEP
                     NRARIAELLDRSLMLVTALAPHIGYDKAAAIAKKAHRDGSTLKEAALALGHVTAQQFD
                     AWVVPATMTRPGTE"
     gene            2500..3324
                     /gene="prmC"
                     /locus_tag="AC16HB_05775"
     CDS             2500..3324
                     /gene="prmC"
                     /locus_tag="AC16HB_05775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5606335.1"
                     /translation="MTVGELLRAAMLPAREARALLACVLRARREWLIAHPEVHVDESQ
                     QAQFATLIERRVRGEPLAYLLGAQEFYGREFEVNPAVLIPRPETELLVDATLDALRGV
                     PVPRILELGTGSGCIAITLALERGDAAVTATDRSAAALGVARANAERLGARVAFIECD
                     WYAGVAGEFDAIVANPPYVAAGDPHLPALRDEPLHALTDHADGLTHLRAIAAGAPAHL
                     SAGASLLVEHGYDQGAAVRALFAAAGLGEVRTLRDAAGIERVCVGRLSEVRAVDRG"
     gene            3460..3780
                     /gene="grxD"
                     /locus_tag="AC16HB_05780"
     CDS             3460..3780
                     /gene="grxD"
                     /locus_tag="AC16HB_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR5606336.1"
                     /translation="MDAKDFIQKTVTENPVVLFMKGTAQFPQCGFSGRAIQVLKACGV
                     KNLITVNVLEDEGVRQSIKEFANWPTIPQLYVKGEFVGGSDIMMEMYQSGELQELLQK
                     AEVA"
     gene            3777..4361
                     /locus_tag="AC16HB_05785"
     CDS             3777..4361
                     /locus_tag="AC16HB_05785"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR5606337.1"
                     /translation="MSAARRRLIVAITGASGAIYGVRLLRHLAARPDVETHLLLSSAG
                     VLNLHQELGLKRAEVERLAHVVHNVNDVGASLESGSFRSAGMVVAPCSMKTLAACALG
                     LADNLITRAADVCLKERRRVVLLVREAPLNLAHIRHMEAVTLMGGVIFPPVPAFYQRP
                     QSIEQLVDHTLARVLDLFDIENDLAPRWQGMGGA"
     gene            complement(4358..5329)
                     /locus_tag="AC16HB_05790"
     CDS             complement(4358..5329)
                     /locus_tag="AC16HB_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5606338.1"
                     /translation="MNRRRLLSAGAAGALLLQAKTAFGQTYPAKPIHLIVGYQPGGQS
                     DLIARHVAKNIGRVLGVSVIVENRPGAGGTIGAEYVARAPADGYVLLLSGGNSLTLAP
                     AMDSRVRYDAQRDFVPVVRVARIPLVLVARADLPVTSLPELIAHARKNPGELTYASGA
                     TIVQVALESLRISDGVEMLHVPYAGSAPAIVDLVAGRVDIGLADVAVVAPHVRSGKLR
                     VLAVADTARARAFPEAATGIEQGVPGFVWDTWAGILAPAGTPTAVVGTLQTAMREALA
                     APDFREGLAKLGFEPIDEDPRAFPDVLSKEAEKFRRLVARIGLRAEN"
     gene            complement(5326..7260)
                     /locus_tag="AC16HB_05795"
     CDS             complement(5326..7260)
                     /locus_tag="AC16HB_05795"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR5606339.1"
                     /translation="MARRWTSRLRVAAKTPVGNSREEFAAIVTTLVVRPTVIENNNIL
                     ATRPRPRHPARTLAQLLEGPIRKHGWREAVLALGRPPLTYARLGEQLEDTARILAAAG
                     YGRGSRIAAALPEGPEFAVVVLALSCAATCAPLSSALDEASLAALLAAMRIDALVTVA
                     GESTVVRAARRASVPLFRLLPCAHDAAGTFQLEVERRRAPVRVEPAAVDDIALLGHTS
                     GTTAIPKIVPYEQWRLAEAVRNLVEQRRYCGDDRSLLLTPLHSLGTTRRILIPPLLVG
                     GSVVCPTALDGRSLVDLLEPLRPTQLTASPAVLRSMLDEFERRQPRPRHALRHIYSGY
                     SELPSPLRQRIEEVFGTTVFLVYGMSEAATIAETPLPPDSAPAYALRWPTTLEVAVAD
                     EAGNHLGAGQTGEIIVRGAEVIEGYENNDEANRQSFQAGWFRTGDAGWIDRDGFLYLS
                     GRIKDIVNRGGMKIGPSDVEKVLVSHPQVAEAAAFGVPHCTLGEDLAAAVVLRDGARA
                     GEGELREFLRSRLPSFKVPAMIFCVPRLPRLSLEKVDRTELARMAEQRVRVDFEAPHA
                     GIESEVARMFAETLAVPRIGRMDNFFHLGGDSLRGVRVLAGVEEAFGLTVSLDLLVDH
                     PTVAGFAGKIGTALSLQSQA"
     gene            7305..7967
                     /locus_tag="AC16HB_05800"
     CDS             7305..7967
                     /locus_tag="AC16HB_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR5606340.1"
                     /translation="MADPIVIFGNGRMAELASLRFGRDPRFEIVGYTVDRTHLNEPRF
                     RGLPIVPFDEVDRHFPAQSVRMFVAVGPVQANRIRAERFLQARALGYRFASHVSPRAS
                     VDPDVRIGENCSIGENAIVGAFTSIGENVFVSAASVVGHHCVLGDHCFVAAGAVVAGT
                     VRVGARAFIGVNATIRDRVAIGEACIIGAGATIVRDTAPGSVHVAPEAVSVPISSDRA
                     RL"
     gene            complement(8005..8080)
                     /locus_tag="AC16HB_05805"
     tRNA            complement(8005..8080)
                     /locus_tag="AC16HB_05805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8045..8047),aa:Thr,seq:tgt)
     gene            8116..9135
                     /gene="galE"
                     /locus_tag="AC16HB_05810"
     CDS             8116..9135
                     /gene="galE"
                     /locus_tag="AC16HB_05810"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR5606341.1"
                     /translation="MSTLLVTGGAGYVGSHTIVELLAAGHRVVCLDNYSNSSPAALAR
                     VQSITGREVVAVEGDLRDAGLLDRLFAENAVDGVLHFAALKAVGESVARPLAYYENNV
                     GGTLTLLAAMQRARVHRLVFSSSATVYGAPDSLPIREDAPIRTMNPYGATKAMVEQIL
                     RDLAASDPAWSFISLRYFNPIGAHPSGLIGEDPHDIPNNLFPFILQVAIGKRARLSVF
                     GNDWPTPDGTGVRDYLHVVDLARGHLRAMEVAERRGGFTAINLGTGHGTSVLELVHAF
                     EAATGQRIAHAITARRPGDIAACWADPTRASQLLQWQAARTIEQACADGWRWQQRNPN
                     GYRGQ"
     gene            complement(9129..9806)
                     /locus_tag="AC16HB_05815"
     CDS             complement(9129..9806)
                     /locus_tag="AC16HB_05815"
                     /inference="COORDINATES: protein motif:HMM:NF027851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HIR5606342.1"
                     /translation="MSMILDALRKADSEREHGSVPGLHSQPVSSLALEEVQPSRTLPW
                     QWIAIGLLSVLVIGLVWLVTNRESQRSAASIAAAESAATHAAAPNPAAVPAPAAIAPA
                     ADRSLEVAEAAPWPQPEARSAPAKSAAPASASARPASPPATGATPPEAIVYAREQLPE
                     AIRAALPALAFGGSMYSSNPANRSLVVNGGLYRENDQLTAELQLEQIKPKAAVFRFRG
                     YRFEVPY"
     gene            complement(9811..>10468)
                     /locus_tag="AC16HB_05820"
     CDS             complement(9811..>10468)
                     /locus_tag="AC16HB_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR5606343.1"
                     /translation="AAARDAAAPAANEPAAFDLPTGFASLVDAEEDSWQALGHAWNTA
                     FKGADPCLAALKQKLQCFRRSNGTLALIRQLDRPVILTLRDRNGRAAYALLEGLSNKT
                     ALLRIDGAPRTVSLVALADYWRGEFATVWRAPPDYSGTGAEFRAGPGARWLAVQLAAA
                     RGEPQPSAGRIDARMLKDWIYAFQLTQGLPLDGLPGPITLMLLNREVGIDEPHLQKAD
                     "
BASE COUNT         1745 a         3559 c         3570 g         1594 t
ORIGIN      
        1 ccgcgtcgca ctccttttgc tcaaagccga tgaagccttc gatgcaagcc aagctcgcgc
       61 acctggcgcg gcgactgaat gaaatcgata ccgcgctggc gagcgaggat gcgacgcgtg
      121 acatgaaccg ctaccgcgcg ctcgggcagg agcgcgccga gatcgagcct gtggtcgcgc
      181 gcttccatga ctacaaccgc gcgcaggccg acctcgccgg tgcacaggag atgctggccg
      241 agccggacat gaaggcgctg gccgaggaag agatccacgt cgcccggggc cggatcgccg
      301 cgctggagga cgacctgcag cgactgctgc tgccgagaga tccgaacgat gagcgcaacg
      361 tgttcctcga gatccgcgcc ggcaccggcg gcgacgagtc ggcgctgttc gccggcgacc
      421 tgctgcgcat gtattcgcgc ttcgccgagc ggcaccggtg gcagaccgaa ctgatctccg
      481 aaagtccgtc cgatctcggc ggctacaagg aggtgatcgt gcgcatcatc ggtgccggcg
      541 cgtactcgaa gctcaaattc gagtccggcg gccaccgcgt gcagcgcgtg cccgagaccg
      601 aggcgcaggg acgcatccac acgtcggcat gcacggtcgc cgtgctgccc gaggccgacg
      661 agatcaacga ggtgaagatc gatccgagcg aactgcgcat cgacgtgttc cgtgcctccg
      721 gcgccggcgg ccagcacgtg aacaagaccg agagcgcggt gcggctcacg cacctgccga
      781 ccgggatcgt ggtcgagtgc caggacgacc gcagccagca caagaaccgc gaacgggcga
      841 tgaagatcct tgccgcgcgc atcaaggaca agcaggtgca ggagcagcgt tcgaaggagg
      901 cgtcgacgcg caagagcctg atcggctccg gcgaccgcag cgagcgcatc cgcacctaca
      961 actttccgca gggccgggtc accgaccacc gcatcaacct gacgctgtac aagatcgacc
     1021 agatcatgga cggcgacctg gacgagatcg ccgccgcgct gaccgcggag catcaggccg
     1081 agctgctggc ggcgctggga gaaacggcat gaccacccga acggagcagg acactttcgg
     1141 cccgatcgaa gtccccgcgg aaagactgtg gggcgcgcag acgcagcggt cgctgcagta
     1201 cttcgcggtg tccaccgaga agatgccgcc ggaactgatc cgcgcactcg tgctgatcaa
     1261 gcgcgccgcg gcaggcgtga acgcagagct cggtctgctc gaagcgcgca agggcgacgc
     1321 gatcgtcgcg gcctgcgacg aggtgctggc cggccggcat gacggcgaat tcccgctgtc
     1381 ggtatggcag accggctccg gcacgcagac caacatgaac gtcaacgagg tgctggccaa
     1441 tcgcgcctcc gaactgctgg gcggagcgcg cggtcccgga cgactggtgc acccgaacga
     1501 cgacgtgaac cgctgccagt cgtccaacga cgtgttcccg acagcgatgc acgtcgcggc
     1561 ggcgctcgcg ctggaccggc aactgcggcc ggcgctgtgc ggcctgcgca ccaccctcga
     1621 tgacaaggcg cgcgcgttcg ccgacatcgt gaagatcggc cgcacgcatc tgcaggacgc
     1681 cacgccgctg acgttcgggc aggagatttc cggctgggcc gctcaactcg aggccgccgg
     1741 cagcgcgatc gtggccgccg agccggccct gctgcggctc gcgatcggtg gcaccgcggt
     1801 cgggacgggg ctgaacgcgc atcccgagtt cggcgcgcgg gtggcggcgc gccttgcggc
     1861 ggataccggc ctcgcctttg cgccggcgcc gaacaaattt gccgcgctcg ccggccacga
     1921 gccgctgctc gccgcgcacg gcgcgctgaa gacgctcgcg gccgcgctga tgaagatcgc
     1981 caacgatgtg cgctggctcg caagcgggcc gcgcgccggc atcggcgagt tgacgatccc
     2041 ggagaacgag ccgggctcgt cgatcatgcc gggaaaggtc aatccaaccc agtgcgagat
     2101 gctgacgatg gtcgccgcgc aggtgctcgg caatgatgtc gcgatcggca tcggcgcggc
     2161 gtcgggcaac ttcgagctga acgtgttcaa gccgctgctc gcgcatgcgt tcctgcagag
     2221 cgtgcggctg ctggccgacg gcagcgcgag cttcgacgcg cattgcgcgc gcggcatcga
     2281 gccgaaccgt gcgcgcatcg cggaactgct cgaccgctcg ctgatgctgg taaccgccct
     2341 cgcgcctcac atcggctacg acaaggcagc ggcaatcgcc aagaaagcgc atcgtgacgg
     2401 cagcacgctg aaggaagcgg cgctcgcgct cggccatgtg accgcgcagc agttcgacgc
     2461 ctgggtcgtg ccggccacga tgacgcgtcc cggcactgaa tgacggtcgg cgagttgctg
     2521 cgcgcggcaa tgctgcccgc gcgcgaggca cgcgcgctgc tggcgtgcgt gctccgcgcg
     2581 cggcgcgaat ggctgattgc gcacccggaa gtacacgtgg atgaatccca acaggcgcag
     2641 ttcgcgacgc tgatcgagcg gcgcgtgcgc ggcgagccgc tcgcgtatct gctgggcgca
     2701 caggagttct acggccgcga attcgaggtg aatcctgccg tgctgattcc gcggccggaa
     2761 acggagctgc tggtcgacgc cacgctcgac gcgctgcggg gcgtgccggt gccgcgcatc
     2821 ctggaactcg gcaccggctc cggctgcatc gcgatcacgc tcgcgctcga gcgcggcgat
     2881 gccgccgtca ccgcgaccga tcgttcggcc gccgcattgg gggtggcgcg cgccaacgcg
     2941 gagcggcttg gcgcgcgtgt ggcgtttatc gaatgcgact ggtacgcggg ggtcgcgggc
     3001 gagttcgatg cgatcgtcgc caatccgcca tacgtcgccg ccggggatcc gcacctgccg
     3061 gcgctgcgcg acgagccgct gcacgcgctg acggaccacg ccgacgggct cacgcacttg
     3121 cgcgcgatcg ccgccggcgc gcccgcgcac ctcagcgcgg gcgcatcgct gctcgtcgag
     3181 catggctacg accagggcgc agcggtgcgt gcgctgttcg cggccgcggg cctcggcgaa
     3241 gtgcggacct tgcgggacgc cgcggggatc gagcgcgtgt gcgttggccg cctgtcagag
     3301 gtccgcgcgg tcgaccgcgg ataacccgac tcgcagcagc gtttatttcg ctggccgcca
     3361 ccctcatgtg caaacgaggc ccgccgctcg ccgccgtttg cgaccgtgtc ggctcggcgc
     3421 ttaaactggc cgcagactca acctgaacag gcgatcgcaa tggacgcaaa ggatttcatc
     3481 cagaagaccg tcaccgagaa tccggtggtt ttgttcatga agggaaccgc gcagttcccg
     3541 caatgcggct tctcgggccg cgcgatccag gtgctgaagg cgtgcggcgt gaagaatctg
     3601 atcacggtca acgtgctcga ggacgaaggc gtgcgccaaa gcatcaagga attcgccaac
     3661 tggccgacga tcccgcagct ctacgtgaaa ggcgaattcg tcggcggctc ggacatcatg
     3721 atggagatgt accagagcgg cgaactgcag gaactgctgc agaaagcgga agtcgcttga
     3781 gtgccgcacg ccgccggctg atcgtcgcca ttaccggcgc ctcgggcgcc atctacggcg
     3841 tgcggttgct gcggcatctt gcggcgcgcc ccgatgtcga aacgcacctg ctgctgtcgt
     3901 cggcgggcgt gctgaacctg caccaggaac tggggctgaa gcgcgccgag gtcgagcggc
     3961 tcgcgcacgt cgtgcacaac gtcaacgacg tcggcgccag cctcgaatcc gggtcgttcc
     4021 gcagcgcggg catggtggtc gcaccgtgct cgatgaagac gctcgccgca tgcgcgttgg
     4081 ggctggccga caacctgatc acgcgcgccg ccgacgtgtg cctgaaggaa cgccggcgcg
     4141 tcgtcctgct ggtgcgcgag gcgccgctga atctcgcgca catccgccac atggaagcgg
     4201 tgacgctgat gggcggcgtg atcttcccgc cggtgcccgc gttctaccag cggccgcagt
     4261 cgatcgagca gttggtcgac cacacgctgg cgcgcgtgct ggacctgttc gacatcgaga
     4321 acgatctggc gccgcggtgg caaggcatgg gcggagccta gttctcggcg cgcaacccga
     4381 tccgggcaac cagcctgcgg aacttctccg cttctttgct caggacgtcc gggaacgctc
     4441 tcgggtcttc gtcgatcggt tcgaaaccca gcttcgcgag gccttcacgg aagtcgggcg
     4501 cagccagcgc ttctcgcatc gccgtctgca gcgtgccgac gaccgccgtc ggcgtgcccg
     4561 cgggagcgag tattccggcc caggtgtccc atacgaagcc cgggaccccc tgctcgattc
     4621 ctgtcgccgc ctccgggaaa gcgcgcgcac gcgcggtgtc ggcgacggcg aggactcgga
     4681 gctttccgga ccgcacgtgc ggcgcgacaa ccgccacgtc cgcgaggcca atgtcgacgc
     4741 ggccagcgac cagatcgacg atggcgggag ccgatcccgc gtacggcacg tggagcatct
     4801 ccacgccgtc cgagatccgg agcgactcga gcgccacctg cacgatcgtc gcgccggatg
     4861 catacgtcaa ttcgcccgga ttcttgcgcg cgtgcgcgat cagttccggc agcgacgtga
     4921 ccggcaggtc ggcccgtgcc accagcacga gcggaatccg cgcaacgcgg acgacgggga
     4981 cgaaatcgcg ttgggcgtca tagcgcacgc ggctgtccat ggcgggtgca agtgtcaggg
     5041 aattgccgcc gctgaggagc agcacgtagc cgtcggctgg cgctcgggca acgtactccg
     5101 caccgatcgt gccgccggcg ccgggccggt tctcgacgat caccgagacg ccaagcacgc
     5161 ggccgatatt cttcgcgaca tggcgtgcta tgaggtcgct ctgcccgccg ggctgatagc
     5221 cgacgatgag gtgaatcggc ttcgcggggt aagtctggcc aaaggccgtc ttcgcttgca
     5281 gcagcagtgc gccggcagcg ccggcgctca gcagtcgacg ccgattcatg cttgactctg
     5341 gagtgagagc gccgtcccga tcttgccggc gaatcccgcg accgtcgggt gatcgaccag
     5401 caggtccagc gaaaccgtca ggccgaaggc ctcttcgacg ccggcaagaa ccctgacgcc
     5461 ccgcaacgag tccccgccga ggtgaaagaa gttgtccatt ctgccgatgc gcggcacggc
     5521 aagcgtttct gcgaacatcc gcgcgacctc gctctcgatt cccgcatgcg gcgcctcgaa
     5581 atcgacccgg acgcgctgtt cggccatccg cgccagttcc gtgcggtcaa ctttctcgag
     5641 tgaaaggcga ggcaaccgcg gcacgcagaa aatcattgcc ggcaccttga acgacggcaa
     5701 ccggctgcgc aggaattcgc gcaactcgcc ctcgccagcc ctggcaccgt cgcgtagcac
     5761 gacagcggcg gcgagatcct cgccgagcgt gcaatgaggc acaccgaagg cggccgcttc
     5821 cgcaacctgc gggtgggaca ccagcacctt ctccacgtcg ctcggcccga tcttcatgcc
     5881 gccgcggttg acgatgtcct tgatccgccc ggacagatag aggaatccgt cgcgatcgat
     5941 ccagcccgca tctccggtgc ggaaccagcc ggcttggaac gattgtcgat tggcttcgtc
     6001 gttgttctcg taaccctcga tcacttcggc accgcggacg atgatttctc ccgtctgtcc
     6061 ggcacccagg tggttcccgg cctcgtcggc gactgcgacc tccagagtcg tcggccacct
     6121 cagtgcgtaa gcaggggcgc tgtcgggcgg cagcggagtc tcggcaatcg tcgcggcttc
     6181 gctcatgccg taaacgagaa agaccgtcgt cccgaagacc tcctcgatcc gttggcgcaa
     6241 cggcgacggc aattccgaat accccgagta gatgtggcgc agcgcatgcc gcgggcgtgg
     6301 ctgccgacgc tcgaactcat cgagcatcga acgaagcact gccggggagg cggtcaactg
     6361 cgtcggccgg agcggttcca gcaggtcgac gagagacctg ccgtccagcg cggtcgggca
     6421 caccaccgat ccgccgacca gcaacggcgg aatcaggatg cgtcgcgtcg ttccaagaga
     6481 atgcagcggt gtcagcagca ggctgcggtc gtcgccacaa tagcgacgct gttcgacaag
     6541 gttgcgcacg gcctccgcaa gccgccactg ctcatacggc acgattttcg gtatcgctgt
     6601 cgtgccggaa gtgtgcccaa gcaacgcgat gtcatcgacc gcagccggct cgacccggac
     6661 cggtgctcgg cggcgctcga cctccaactg gaacgtgccc gcggcgtcgt gcgcgcatgg
     6721 aagcagccga aacaacggca ccgatgcgcg acgcgcggcg cggactactg tcgattcccc
     6781 cgccaccgtc acgagcgcat cgatgcgcat cgccgcgagc aacgcagcga gcgacgcttc
     6841 gtcgagcgcg ctgctcagcg gagcgcaggt ggccgcgcaa ctcaacgcaa gtacgacgac
     6901 ggcgaactca gggccctcgg gcagcgccgc cgcgatgcgc gatccgcgtc cgtagccggc
     6961 tgcagccaga atacgtgccg tgtcttcgag ttgctcgccc aaccgcgcgt aggtcagcgg
     7021 cgggcggccc agcgcgagta ccgcctcgcg ccagccatgc ttgcgaatcg ggccttcgag
     7081 caattgcgcg agcgtgcgtg ccggatgtcg tggtcgcggt cgcgtcgcta gaatgttgtt
     7141 gttttcgatc acggtcggtc ggacgacgag agtggtaacg atcgcggcga attcttcccg
     7201 gctgttcccc acgggagtct tcgccgcaac gcgcaggcgc gatgtccagc ggcgcgccac
     7261 tatagcccga acaacgatga atgattcgcc cgctcgactc gtgaatggct gatcccatcg
     7321 tcatcttcgg caatggccgg atggcagagc ttgcctccct gcgattcggt cgtgatcccc
     7381 gtttcgagat cgtcggctac accgtagacc gaacgcacct caatgaaccg cggttccggg
     7441 gtctgccgat cgttccgttc gacgaggtgg accgccactt tccggcgcag tcagtgcgca
     7501 tgttcgtcgc cgtcggaccg gtgcaggcca accgcatccg cgccgagcgc tttctgcagg
     7561 cgagagccct cgggtatcgc ttcgcaagtc acgtcagtcc acgcgcgagc gtcgatcccg
     7621 atgtccgcat cggcgagaac tgctcgatcg gcgaaaacgc gatcgtcggc gcgttcactt
     7681 ccatcggcga aaacgttttc gtcagcgcgg ccagcgtggt cggccaccac tgtgtgctcg
     7741 gcgatcactg cttcgtggcc gcgggcgccg tcgtcgccgg cacggtgcgc gtgggagcac
     7801 gtgccttcat cggcgtcaac gcgacgatcc gcgaccgggt cgccatcggc gaggcctgca
     7861 tcatcggagc cggagcgacg atcgtgcgag acaccgcccc cggcagcgtt cacgtggcgc
     7921 ccgaagcggt cagcgtgccc atcagcagcg atcgggccag gctgtgatcg gggcggcgca
     7981 acccgagccc gcatagggat cgcgtggtgc cggttatccg actcgaacgg atgacctatc
     8041 gcttacaagg cgattgctct accaactgag ctaaaccggc gcgggcgaaa gtctagcccg
     8101 gatatcatgg ctccgatgag cactctcctc gtgacgggcg gcgccggcta cgttggatcc
     8161 cacacgatcg tcgagctgct ggccgcggga caccgcgtcg tctgcctcga caactactcg
     8221 aacagttcgc cggcggctct cgcgcgcgtg cagtcgatca ccggccgcga ggtggtcgcg
     8281 gtcgagggtg acctgcgtga cgcggggctg ctcgaccgct tgttcgccga gaacgccgtc
     8341 gacggcgtgc ttcatttcgc ggcgctgaag gcagtcggcg aatcagtcgc gcggccgctc
     8401 gcttactacg agaacaacgt cggcggcacg ttgacgctgc tggcggcgat gcagcgcgcg
     8461 cgtgtgcacc ggctggtatt cagctcgtcg gcgaccgtct acggcgcgcc ggattccttg
     8521 ccgatccgcg aagatgcgcc gatccgcacg atgaacccgt acggcgcaac caaggcgatg
     8581 gtcgagcaga tcctgcgcga cctcgcagcg tcggacccgg catggtcgtt catcagcctg
     8641 cgctatttca acccgatcgg cgcgcacccg agcggcctga tcggcgagga tccgcacgac
     8701 atccccaaca acctgttccc gttcatcctg caagtggcga tcggcaagcg cgcgcgtcta
     8761 tcggtgttcg gcaacgactg gcccacgccg gacggcacgg gcgtgcgcga ttacctgcac
     8821 gtggtcgacc tggcgcgcgg tcacctgcgc gccatggagg tcgccgagcg ccgcggcggc
     8881 ttcacagcga tcaacctcgg cacgggacac ggcaccagcg tgctggaact ggtgcatgcg
     8941 ttcgaagcgg cgaccggcca gcgcatcgcc catgcgatca ccgcacgccg acccggcgac
     9001 atcgcggcct gctgggccga tccgacgcgc gcgtcgcagt tgttgcagtg gcaagccgcg
     9061 cgcacgatcg agcaggcgtg cgccgatggc tggcgctggc agcagcgcaa tccgaacggc
     9121 taccgcggtc agtaaggaac ttcgaaccga tagccgcgga agcggaagac cgccgccttc
     9181 ggcttgatct gctcgagctg aagctcggcc gtcaactggt cgttctcgcg atacagaccg
     9241 ccgttgacga ccagcgaacg gttggccgga ttgctcgaat acatcgagcc gccgaacgcc
     9301 agtgccggca gcgcggcgcg gatggcctcc ggcaactgtt cgcgcgcgta gacgattgcc
     9361 tcgggcggcg tggcgccggt cgccggcggc gacgccgggc gggcggatgc ggacgccggc
     9421 gcggccgatt ttgcaggcgc gctgcgcgcc tccggctgcg gccacggcgc cgcctcggcc
     9481 acctccagcg atcggtcggc cgccggagcg atcgcggcag gagccggcac tgcagcgggg
     9541 ttcggtgcgg cggcatgcgt ggccgccgat tccgccgccg caatcgacgc cgcggatctc
     9601 tgtgattcac ggttcgtgac gagccacacc agcccgatca cgagcacgga aagcagaccg
     9661 atcgcgatcc attgccaggg cagcgtgcgc gacggctgca cctcctccag cgcaagcgac
     9721 gacaccggtt gcgagtgcaa gccgggcacc gaaccatgct cgcgctccga gtcggccttg
     9781 cgcagggcat cgagaatcat cgacatgggg ctagtcggcc ttctgcaggt gcggctcgtc
     9841 gatgccgacc tcgcgattca acagcatcag cgtgatcggg ccgggaaggc catccagcgg
     9901 cagtccctgg gtcagctgga acgcgtagat ccaatccttg agcatcctgg cgtcgatgcg
     9961 gccggcgcta ggctgcggct cgccgcgcgc tgcggcgagc tgcacggcca gccagcgcgc
    10021 gccggggccg gcgcggaatt cggcgccggt gccggagtaa tcgggcggcg cgcgccagac
    10081 ggtcgcgaac tctccgcgcc agtaatcagc gagcgcgacg agcgacaccg tgcgcggcgc
    10141 gccgtcgatg cgcagcagcg cggtcttgtt cgacagcccc tcgagcagcg cgtatgcagc
    10201 acggccgttg cggtcgcgca gcgtcaggat gaccgggcgg tcgagttgcc ggatcagcgc
    10261 cagcgtgccg ttgctgcgcc ggaagcactg cagcttctgc ttcagcgcgg ccagacacgg
    10321 atccgcgccc ttgaacgcgg tgttccatgc gtgccccagc gcctgccagg agtcttcctc
    10381 ggcgtcgacc agggatgcga accctgtcgg cagatcgaat gcggccggtt cgttcgcggc
    10441 tggcgcggca gcgtcgcgcg ctgcggcg
//
LOCUS       DBNHBY010000232         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1298938, whole genome shotgun sequence.
ACCESSION   DBNHBY010000232 DBNHBY010000000
VERSION     DBNHBY010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1298938"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..812
                     /locus_tag="AC16HB_05825"
     CDS             153..812
                     /locus_tag="AC16HB_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5606344.1"
                     /translation="MGGQVHGRLCELLVSGVLSPGEKLSLRTVAERLGVSITPVRAAV
                     ARLITDHALEVAPNRAVRVPVVSLPQFRELTAIRLAIEGFAAERAARHRTDGDLTAMR
                     RHDAAFRHQCRSGRPDAAAAVKANQEFHFAVYRAAGLPSLMPIIEGLWLRIGPVLNLD
                     MRSSAQRLRIGAAERCHAELLREIELSDGPAARAALAADIEGAAAFIESRGVLPDEAP
                     H"
     gene            827..1603
                     /locus_tag="AC16HB_05830"
     CDS             827..1603
                     /locus_tag="AC16HB_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606345.1"
                     /translation="MNLQIEGLRVMISAGAAGIGLEIARAFVGEGARVHVCDVDEAAP
                     AALARSDPKLSWTRCDVASRVDVACFFDDGVAKLGGLDCLINNAGIAGPTGRVDEIDP
                     DEWDRCIAIDLTGQFNCIRLAVPHLRQSSNASIMNVSSLGGRMGFPLRTPYASAKWGV
                     IGLARSLAMELGPDRIRVNALTPGIVAGERQRRVLSAKAQARGISFEAMEQLAFSSTS
                     LKEYVTPQQLADLVVFVASPRGRLISGQVLSVCGDTQMLG"
     gene            complement(1610..1882)
                     /locus_tag="AC16HB_05835"
                     /pseudo
     CDS             complement(1610..1882)
                     /locus_tag="AC16HB_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
BASE COUNT          302 a          676 c          709 g          343 t
ORIGIN      
        1 gccggccgcg cttgccgaaa cggtgatcac cgcttgcttg cggcaaactg tgatcacaat
       61 tatcgtggat caaggaggcg gatttgtcca gcgcgatcgc gaaacaccgg ggcacagcga
      121 aacggacgca cgtccgttcg gcgcgtccga ccatgggtgg acaggtgcac ggccgcctgt
      181 gcgagttgct ggtctccggc gtgctctcgc cgggcgagaa gctgtcgctg cgcacggtgg
      241 ccgaacgcct cggcgtatcc atcacgccgg tgcgcgccgc ggtcgcgcgg ctgatcaccg
      301 atcacgcgct cgaggtggct ccgaaccgcg cggtgcgcgt gccggtcgtg tcgctgccgc
      361 aattccgcga actgaccgcg atccggctgg cgatcgaagg ctttgcggcc gaacgcgccg
      421 cccggcatcg aaccgacggc gacctcacgg cgatgcgccg gcacgacgcc gccttccgcc
      481 accagtgccg cagcggtagg cccgatgctg cggccgcggt caaggcaaac caggaattcc
      541 acttcgccgt ctaccgcgcg gcggggctgc cgtcgctgat gccgatcatc gaggggctgt
      601 ggctgcgcat cgggccggtg ctgaacctcg acatgcgttc gagtgcgcag cgtctgcgca
      661 tcggcgccgc cgagcgttgc cacgcggaac tgctgcgcga gatcgagctg agtgacgggc
      721 cggccgcgcg cgcggcgctg gcggccgaca tcgagggcgc ggccgccttc atcgaatcgc
      781 gcggcgtgct gccggacgaa gcgccgcatt gacggaaaga ttccgcatga acctgcagat
      841 cgaaggcttg cgcgtgatga tctcggcggg ggctgccggc atcgggctgg agatcgcgcg
      901 cgcattcgtg ggcgagggcg cgcgcgtgca cgtgtgcgac gtcgacgagg cggcgcccgc
      961 ggcgctggca cgctccgatc cgaagctgag ctggactcgc tgcgatgtcg cgtcgcgcgt
     1021 cgacgtggcg tgcttcttcg acgacggcgt cgcgaagctc ggggggctcg actgcctgat
     1081 taacaacgct ggcatcgccg ggcccaccgg acgtgtcgac gagatcgatc cggacgaatg
     1141 ggaccgctgc atagcaatcg acctcaccgg ccagttcaac tgcatcaggc tcgcggtgcc
     1201 gcacctgcgg caatcgtcga acgccagcat catgaacgtg tcgtcgctcg gcgggcgcat
     1261 ggggtttccg ctgcgaacgc cgtacgcgtc ggcgaaatgg ggcgtgatcg gactggcgcg
     1321 ctcgctggcg atggaactgg ggccggaccg cattcgcgtc aatgcgctga cccccggcat
     1381 cgtcgcgggc gagcggcagc ggcgcgtgct atcggccaag gcgcaggcac gcggcatctc
     1441 gttcgaggcg atggagcagc tcgcgttctc ctccacgtcg ctgaaggagt acgtgacccc
     1501 gcagcaactc gcggatctgg tcgtgttcgt cgccagccca cgcgggcggc tgatctcggg
     1561 tcaggtgctg tcggtctgcg gcgacacgca gatgctcggc tgaccgccgt tacctgcccg
     1621 cgtacgcggt cacggcctgc cgttgctcgc ccagcccggc gatgccgagc gtgacgacat
     1681 cgccggcctt caggtactgg ggcggcttca tccccaggcc cacgcccggc ggcgtgccgg
     1741 tggtgatgat gtcgcccggc agcagggtca tgaactggct gatgtagctg accagttgcg
     1801 ccacgccgaa caccatcgtg cgcgtggtgc cgcgctggcg cttctcgccg ttgacgtcga
     1861 gccacatgtt caggttcgtc cctgatctcg cccttgccct tgtgccactg gccgttcatt
     1921 gatgccttga agccgggcgc cgtctttgct agcggcttga gcacatcccg aactttatcg
     1981 gccattatgg aaggtcatcg ccggatcatg cgcattgcag ccatcaatag
//
LOCUS       DBNHBY010000233         3067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1502224, whole genome shotgun sequence.
ACCESSION   DBNHBY010000233 DBNHBY010000000
VERSION     DBNHBY010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1502224"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16HB_05840"
     CDS             <1..215
                     /locus_tag="AC16HB_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606346.1"
                     /translation="RDEFLDLLDAEIVSHVTHRSGSIHGRVSVVSARPAKKNRQVSGL
                     AVGVGFGLGYARRSTLPARARKKPKK"
     gene            complement(316..1050)
                     /locus_tag="AC16HB_05845"
     CDS             complement(316..1050)
                     /locus_tag="AC16HB_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606347.1"
                     /translation="MNASQRRGCRPGRALATCAALCLLAGPAVAQVTIGAAGDNPALV
                     APPSYQLIDTAFGTPLRSAQPLGLWRLEATTSLAPRHELRFSTWAIGRGGLESLPGVS
                     LATLASDASLRVDPARATYRYTFFAQRDWAWKLGISANVKEFGDTLRPGLGFSDRLRF
                     GALPLLHVGAEGQLASRWRLAFDADGLMTPRGRTFDLGLRVNYTLTPNFLLFGGYRLT
                     DSAGDAEEFYGAGLSNAANFGLRYRF"
     gene            complement(1171..2112)
                     /locus_tag="AC16HB_05850"
     CDS             complement(1171..2112)
                     /locus_tag="AC16HB_05850"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5606348.1"
                     /translation="MRPYETFFVVLLGMVLLIATATLLGNWLATRAADAAKLAWIGQL
                     NSRIRASWLIVLLFALGFAVGGGALVVLFAFASFFALREFVALTPIRPSDHWALLIAF
                     YLVIPIQYLLVAGEGYGVFSVFIPVYVFLLLPVVMALKQDTVDYLQRVAKMQWGLMIS
                     VYCVSHAPAIASIEMPGYEGRGALLLLYFLLVLQMSDLLAVIASAALGRTPLKSNPNK
                     SKEGVLLGGIGATLVGTALWWMTPFTWSESTLMSASIVAAGVMGGLVLNSVKRGLGAR
                     DWAEDGVLLSRGVLDRLDALTFAAPVFFHLTLYFFVS"
     gene            complement(2126..2743)
                     /locus_tag="AC16HB_05855"
     CDS             complement(2126..2743)
                     /locus_tag="AC16HB_05855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5606349.1"
                     /translation="MRIPFPKFLPPIVWLVRLLVGAYPRWIGSTPKATQRIYFANHTS
                     HMDTVVLWAALPGALRVNTRPVAAKDYWGGGGLKTRIAQDELNVVMIERAREERTGDP
                     LDPLRDALNHGFSLIIFPEGTRTPQPLPGQFRSGLYTLAQEFPQVELIPVYIENLHRS
                     MPKGALLPVPIICTVRFGAALALSEGESKEQFLSRARNAVIALAD"
     gene            complement(2764..>3067)
                     /locus_tag="AC16HB_05860"
     CDS             complement(2764..>3067)
                     /locus_tag="AC16HB_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR5606350.1"
                     /translation="VKKGLYATNFGGGQVDITNGKFVFSMAEAYLIENGRVTAPVKGA
                     TLIGNGPDALTRVTMVGNDMQLDPGIGVCGKDGQSVPVGVGQPTLRIEGLTIGGTG"
BASE COUNT          568 a         1018 c         1011 g          470 t
ORIGIN      
        1 ggcgggacga gttccttgat ctccttgatg cggagatcgt cagtcatgta acgcatcggt
       61 cgggctccat ccatggcagg gtttccgttg tttcggcccg cccggcgaaa aaaaaccgcc
      121 aggtttcggg cctggcggtg ggtgtcggat tcggtctagg ttacgcgcgc cgctcgactc
      181 ttcccgccag agcccggaaa aagccaaaga agtaaaagaa gaagcgcgcc gaagagagca
      241 tgtgcgtcgt tcgtatcgtt gcgatccgga ctttacccgg cttgacgcgg cgcggcaagc
      301 aaggtttcgg gccgctcaga accggtagcg caggccgaag ttcgcggcgt tcgacaagcc
      361 cgcgccatag aactcctcgg cgtcgccggc cgaatcggtc aggcggtaac cgccgaagag
      421 caggaagttc ggcgtcaacg tgtagttgac gcgcaggccg aggtcgaacg tacggccgcg
      481 cggcgtcatc agtccgtcgg catcgaacgc aaggcgccag cgcgacgcaa gctggccctc
      541 ggcgcccacg tgcaacagcg gcagtgcgcc gaagcgcagt cggtccgaga atcccaggcc
      601 gggccgcagg gtgtcaccga actctttcac gttggccgag atgccgagct tccatgccca
      661 gtcgcgttgc gcgaagaacg tgtagcgata ggtggcgcgc gcgggatcga cgcgcagcga
      721 agcgtcgctt gccagcgttg ccagagaaac gcccggcagc gactcgagtc cgccgcggcc
      781 gattgcccac gtgctgaagc gcagttcgtg tcgcggcgcg agcgacgtcg tcgcctccaa
      841 tcgccacagg cccagcggct gcgctgagcg cagcggtgta ccgaaagccg tgtcgatcag
      901 ttggtacgac ggcggcgcga ccagcgccgg attgtctccg gcggcgccga tcgtcacctg
      961 tgcgaccgcc ggacccgcca acaggcacag cgccgcgcac gttgcaagcg cgcggccggg
     1021 ccggcaacct cgcctctgac tcgcgttcat gacgtcaccc tgggaaccca cgcatcgccg
     1081 tcgtggcgtg tgacaggaat attcgccttc cgccgcgggt gtgcaagcac ttgccgcaac
     1141 aaggcgcgat ctacttgacg tatggcggcg tcaggagacg aaaaaataca acgtcaggtg
     1201 gaagaacacc ggcgccgcaa aggtcagcgc gtcaagccgg tccagcacgc cgcgcgacag
     1261 caaaacgccg tcttcagccc agtcgcgcgc gcccaggccg cgtttgacgg agttgagcac
     1321 caatcccccc atgacgccgg cggcgacgat ggaagccgac atcaacgtcg attcggacca
     1381 cgtgaacggg gtcatccacc acaatgcggt gccgacgagt gttgcgccga tgccaccgag
     1441 cagcacgcct tccttgctct tgttcgggtt ggacttcagc ggcgtgcggc cgagcgcggc
     1501 gctggcgatc accgccagca agtccgacat ctgcagaacc agcaggaagt acagaagcag
     1561 cagtgcgccg cggccctcgt agccgggcat ctcgatgctc gcgatcgccg gcgcatggct
     1621 gacgcagtag acgctgatca tcagtcccca ttgcatcttg gccacgcgct gcaggtagtc
     1681 cacggtgtcc tgcttcagcg ccatcacgac cggcaatagc aggaatacgt acaccgggat
     1741 gaacacggaa aagacgccgt acccctcgcc ggcgacgagc aggtattgga tcggaatcac
     1801 caggtagaac gcgatcagca gcgcccagtg gtcgctcgga cggatcggcg tcagggcgac
     1861 gaactcgcgc agcgcgaaga acgacgcgaa cgcgaacagc acgaccagcg cgccgccgcc
     1921 gaccgcgaag ccgagcgcga acagcagcac gatcaaccac gaggcgcgga tgcgcgaatt
     1981 cagttgcccg atccacgcca gcttggcggc gtccgccgcg cgggtggcga gccagttgcc
     2041 cagcagcgtg gcggtcgcga tcagcagcac catgccgagc agcacgacaa agaaggtttc
     2101 gtagggcctc atgtcaatcg ctgtatcaat ccgcgagcgc gatcaccgca ttgcgtgcgc
     2161 gcgacaggaa ctgctccttc gattcgccct cggacagcgc cagcgccgcg ccgaagcgca
     2221 cggtgcagat gatcggcacc ggcagcagcg cgcccttggg catgctgcgg tggaggttct
     2281 cgatgtacac ggggatcagt tcgacctgcg gaaactcctg cgccagcgtg tacagcccgc
     2341 tgcgaaactg ccccggcagc ggctgtggtg tgcgcgtacc ctcgggaaag atgatcagcg
     2401 aaaagccgtg gttcagcgcg tcgcgcagcg ggtcgagcgg gtcgccggta cgctcctcgc
     2461 gcgcgcgctc gatcatcacg acgttcaact cgtcctgcgc gatgcgcgtc ttcagcccgc
     2521 cgccgcccca gtagtccttg gccgcgaccg ggcgcgtgtt gacgcgcagc gctcccggca
     2581 gggcagccca cagcacgacc gtgtccatgt ggctggtgtg attggcgaag tagatgcgct
     2641 gtgtggcctt cggcgtcgag ccgatccagc gcgggtacgc gcccaccagc aggcgcacca
     2701 gccagacgat cggcggcagg aatttcggga acggaattcg caagagttgc gcgctcgagg
     2761 cgctcagccg gtgccgccta tggtcagccc ttcgatgcgc agcgtcggct ggcccacgcc
     2821 gaccggcacg ctctggccgt ccttgccgca cacgccgatg ccggggtcga gctgcatgtc
     2881 gttgccgacc atcgtcacgc gcgtcagcgc gtcggggccg ttgccgatca gcgtggcgcc
     2941 cttgacgggc gcggtgacgc gaccgttctc gatcaggtac gcctcggcca tgctgaacac
     3001 gaacttgccg ttggtgatgt cgacctgccc gccgccgaag ttggtagcgt acaacccctt
     3061 cttgacc
//
LOCUS       DBNHBY010000234         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1163723, whole genome shotgun sequence.
ACCESSION   DBNHBY010000234 DBNHBY010000000
VERSION     DBNHBY010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1163723"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="argS"
                     /locus_tag="AC16HB_05865"
     CDS             complement(<1..531)
                     /gene="argS"
                     /locus_tag="AC16HB_05865"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR5606351.1"
                     /translation="MLPQQRQAIVGLIRDALAAFSAPEIPIELTRPKVAAHGDLACAV
                     ALQLARQLKQSPRDVAQAIVDNLKLNPQSKGLLDAVEVAGPGFINLRVAASAKQDVVS
                     AVLRERDRFGTSNAAHGQKVMVEFVSANPTGPLHLGHARQAALGDALANLLATQGWDV
                     TREFYYNDAGVQIDTLA"
     gene            597..1226
                     /locus_tag="AC16HB_05870"
     CDS             597..1226
                     /locus_tag="AC16HB_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5606352.1"
                     /translation="MRSQVDHLVVACGDLEQGAAWLEQRLAVAAQPGGKHALMGTHNR
                     LLKLGARSYLELIALDPEGNAMRPRWFALDDPAIQRRAREQPFLLTWAAATTSIVEAV
                     VQVPELGEVIAATRNQFAWRITVPDDGRLNFSGVLPTVIEWDGDVHPCDVLPDSGCEL
                     LELRLSHPAASSIVPMFRSLRLAGAVAVRPGPIELAARVRSPRGEVDLR"
     gene            complement(1228..>2208)
                     /locus_tag="AC16HB_05875"
     CDS             complement(1228..>2208)
                     /locus_tag="AC16HB_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR5606353.1"
                     /translation="PLVVATRGDLPELVHVGSIAVVDAGGKLIAGVGDPTAFNFTRSA
                     LKPLQALPFVADGGMDRFAFTSHELALMCASHSGEAIHVATVQRMLDRIGLGEPDLQC
                     GCHPPTYYVHTATQAPPNARWNQLHHNCSGKHSGFLAWCRLHDAPTKSYLDPEASLQR
                     RVRDVVRSYAAGSEPPLGIDGCSAPNFALPLTGLAAAFRDLATGKTREHAALAYAMTH
                     HPDLVSGTARSDLALMQAGNGDWVTKIGADGVQAIGVRSKGIGIAIRMADGNTRALLA
                     AAVEVLQQLGVLADASTTPLARYMRPQIKNYRGTVVGHVEPLFALPRVSV"
BASE COUNT          322 a          740 c          786 g          360 t
ORIGIN      
        1 cgccagcgtg tcgatctgca cgccggcgtc gttgtaatag aactcgcgcg taacgtccca
       61 gccctgtgtg gcgagcagat tggcgagtgc atcgcccagc gccgcctgcc gtgcgtgacc
      121 gaggtgcagc ggcccggtcg ggttggccga cacgaactcg accatcacct tctgtccgtg
      181 cgccgcgttg ctggtgccga agcggtcgcg ctcgcgcagc accgcggaca cgacgtcctg
      241 cttggcactc gccgccaccc gcaggttgat gaagccggga ccggcgacct cgaccgcatc
      301 gagcagcccc ttggattgcg ggttcagttt caggttgtcg acgatcgcct gcgcgacatc
      361 gcgcgggctc tgcttcaact gcctggcgag ttgcagcgcc accgcgcacg ccaggtcgcc
      421 gtgcgcggcg accttggggc gcgtcagttc gatcgggatc tcgggagcgg aaaatgcggc
      481 gagcgcgtcg cgtatcagtc cgacgatcgc ctgccgttgc tgcggaagca tgtccgggct
      541 gacgcaatgt gcagaggagc gaattctacc cgcgcggctg ccctaaactc gtcgcgatga
      601 gaagccaagt cgatcatctg gtggtggcgt gcggcgatct ggagcagggc gcagcctggc
      661 tggaacagcg gctcgcggtg gcggcgcagc ccggtggcaa gcatgcgctg atgggcacgc
      721 acaaccgatt gctgaaactc ggtgcgcgga gctacctcga actgatcgcg ctcgatccgg
      781 agggcaacgc aatgcgcccg cgctggttcg cgctcgacga ccccgcgatc cagcggcgcg
      841 cgcgcgagca gccgtttctg ctgacctggg cggcggcgac gaccagcatc gtcgaggcgg
      901 tggtgcaggt gccggagctg ggcgaggtca tcgcagcgac gcgcaaccag ttcgcgtggc
      961 gcatcacggt gccggacgac gggcgtctga atttctccgg cgtgctgccg acggtgatcg
     1021 aatgggacgg cgacgtgcat ccgtgcgacg tgctgcccga cagcggctgc gagctgctgg
     1081 aactgcggct gtcgcatccg gccgccagca gcatcgtgcc gatgttccgc tcgctgcggc
     1141 tggccggcgc ggtcgccgtc cggcccgggc cgatcgaact cgcggcgcgg gtccgctcgc
     1201 cgcgcggcga ggtcgatcta cgctgaatca gaccgaaacg cgcggcagcg cgaagagcgg
     1261 ctcgacgtgg ccgacgacgg tgccgcggta gttcttgatc tgcggccgca tgtaccttgc
     1321 cagcggcgtc gtcgatgcgt cggcaagcac gcccagttgc tgcagcacct cgaccgccgc
     1381 cgcgagcagc gcgcgcgtgt tgccgtccgc catgcggatc gcgatgccga tgcccttgct
     1441 cctcaccccg attgcctgca cgccatcggc gccgatcttg gtgacccagt cgccgttgcc
     1501 cgcctgcatc agtgcgagat cgctgcgtgc ggtgcccgac accaggtccg gatggtgtgt
     1561 catcgcgtac gcgagcgccg cgtgttcgcg ggtcttgccg gtcgcgagat cgcggaacgc
     1621 tgcagcgagg ccagtgagcg gcagcgcgaa attcggcgcg ctgcagccgt cgatgcccag
     1681 cggcggttcg ctgcccgccg cgtagctgcg taccacgtcg cgcactcggc gttgcagcga
     1741 tgcctcgggg tcgaggtagc tcttcgtcgg tgcatcgtgc agccggcacc acgcaaggaa
     1801 gccgctgtgc ttgccggagc agttgtggtg cagttggttc cagcgcgcgt tcggcggcgc
     1861 ctgcgtcgcg gtgtgcacgt aatacgtggg tgggtggcag ccgcactgca ggtccggctc
     1921 gcccagcccg atgcggtcga gcatgcgctg cacggtggcg acgtggatcg cctcgccgct
     1981 gtggctcgcg cacatcaggg ccagctcgtg cgacgtgaat gcgaaacggt ccatgccacc
     2041 gtcggcgacg aaaggcagtg cctgcagcgg cttcagcgcc gagcgcgtga aattgaacgc
     2101 ggtcggatcg ccgacgccgg cgatcaactt gccacctgca tccaccaccg cgatcgagcc
     2161 gacgtgcacc agttccggca ggtcgccgcg cgtggcgacg accagcgg
//
LOCUS       DBNHBY010000235         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_352080, whole genome shotgun sequence.
ACCESSION   DBNHBY010000235 DBNHBY010000000
VERSION     DBNHBY010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_352080"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="AC16HB_05880"
     CDS             <1..1272
                     /locus_tag="AC16HB_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606354.1"
                     /translation="ALRHYAEAAESALRHFAPSDAMSLAAQALDVLPHAPAGPMRDAL
                     EFAHCALTGVSAAQSLGVSSVDTKRAFERALALLPGLPQHPMRGLVLHGLGVALLVRG
                     EYAQAIALAERTKSLADASGDRVLLLSACNVLGQIHSMQGRPQQAREWLERGVAACEA
                     LGDRAMQAAFIIDPGVTMSAALAMPLLHLGFVGEARARLQAAHARAHRLGQPMARMVA
                     HWFSALAEIRLDDVERVAAHADEIRVIVEGAALAQGHAIHRWLRGWALARRGEPREGY
                     RLIREAYERNTRLDTLVGATEVLGYATEALLLDRDLPGAEAQLDEAMQLGARLGERVY
                     LPQLWVLQARIADAQGRPDTAREAMRQALHEARDQQAAWLELNVLTAQCGRKEATAAD
                     FESLAAVCARLSEGLDTAPVAQARALLNSRT"
     gene            1426..1626
                     /locus_tag="AC16HB_05885"
     CDS             1426..1626
                     /locus_tag="AC16HB_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606355.1"
                     /translation="MTLDPQSKYPISRSYVLKLHRDARPRSDALAGRLENMMTGNHRE
                     FASGEQLLALLASELDSIPIRT"
     gene            1643..>1974
                     /locus_tag="AC16HB_05890"
     CDS             1643..>1974
                     /locus_tag="AC16HB_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024978.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HIR5606356.1"
                     /translation="MRLLPLPGSAPSVLSATLRAGCALAALAAVLSGCASSPKIMVDK
                     DPAVDLKHYRTFGYFDKVATDKTQYSTLLTSHLKRATRAQLERHGYAYAEENPDLRVN
                     FFLNVADK"
BASE COUNT          314 a          690 c          670 g          300 t
ORIGIN      
        1 gcgctgcgcc actatgcgga agcggcagag agcgcattgc gtcactttgc gccgagcgac
       61 gccatgagcc tggccgcgca ggcgctcgac gtcttgccgc acgcaccggc cggccccatg
      121 cgcgacgcgc tggagtttgc gcactgtgca ttgaccggag tgtccgcggc gcagtcgctc
      181 ggcgtcagct ctgtcgacac caagcgcgcc ttcgagcgcg cgctggcgct gcttcccggc
      241 ttgccgcagc acccgatgcg cgggctcgtg ctgcacgggc tcggggtggc gctgttggtg
      301 cgcggcgagt acgcgcaggc gatcgcgctg gccgagcgca ccaagtcgct cgccgatgcc
      361 agcggcgacc gcgtgttgct gctatcggcc tgcaacgtac tcggccagat ccattcgatg
      421 cagggccggc cgcagcaagc gcgcgaatgg ctcgaacgcg gcgtggcggc ctgcgaagcg
      481 ctcggtgatc gcgcgatgca ggccgcgttc atcatcgatc ccggcgtgac gatgtctgcg
      541 gcgctcgcga tgccgctgct gcacctggga ttcgtcggcg aggcacgcgc gcgcctgcaa
      601 gccgcccatg cgcgcgcgca tcgacttggc cagccgatgg cgcggatggt cgcccactgg
      661 ttcagcgcgt tagcggagat acgcctggac gacgtcgaac gggtggccgc gcacgccgac
      721 gagatacgcg tgatcgtcga aggcgccgcg ctcgcgcagg gtcacgcgat ccatcgctgg
      781 ctgcgcggct gggcgctggc acggcgcggc gagccgcgcg agggctatcg cctgatccgc
      841 gaagcgtacg agcgcaacac gcgcctcgac acgttggtcg gcgccaccga ggtgctcggt
      901 tatgcgaccg aggcgctgct cctcgatcgc gatttgcccg gcgcggaagc acagctcgac
      961 gaagcgatgc aacttggcgc cagactcggc gagcgcgttt acctgccgca actgtgggtg
     1021 ctgcaggcgc gcatcgcgga cgcgcagggc aggcccgaca ctgcgcgcga agcgatgcgg
     1081 caggcgttgc acgaggcacg cgaccagcag gcagcgtggc tggaattgaa cgtactgacc
     1141 gcgcaatgcg ggcgcaaaga agcgacggcg gcggacttcg agtcgctcgc ggccgtatgc
     1201 gcacgtctga gcgaaggtct cgacaccgcg ccggtcgcgc aggcgcgcgc gttgctcaac
     1261 tcacgcacgt gaccattgtc cgggccgact gacgcccggc agttccgccc gcgtccccgc
     1321 ggagcggcgc aaaccggacc gaaacccgac cgtttcctga cgacgccgtc ggcgccggtt
     1381 cttacgctgc cgcccatcga ttcacggttg atgacggctg tcccgatgac ccttgatccg
     1441 caatccaagt acccgatttc gcgcagctac gtgctgaagc tgcaccgcga tgcgcggccg
     1501 cgatccgacg cgctggcggg ccggctggaa aacatgatga ccggcaacca tcgcgagttc
     1561 gcgagcggcg agcagttgct cgcgctgctg gcgagcgagc tcgattcgat cccgatccgc
     1621 acctgacttg aaggagagac tcatgcgcct gctccccctc cccggctccg ccccgtccgt
     1681 gttgtccgcc accctccggg ccggatgcgc attggccgcg ctggcggcgg tcctgtccgg
     1741 ctgcgcttcc agcccgaaga tcatggtcga caaggacccc gcggtcgatc tgaagcacta
     1801 caggaccttc ggctacttcg acaaagtcgc gaccgacaag acccagtatt cgacgctcct
     1861 gacatcgcat ctgaagcgcg cgacgcgcgc ccagctcgaa cggcacggct acgcatacgc
     1921 cgaggagaac ccggatctgc gggtcaactt cttcctcaac gtcgccgaca agca
//
LOCUS       DBNHBY010000236         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_148593, whole genome shotgun sequence.
ACCESSION   DBNHBY010000236 DBNHBY010000000
VERSION     DBNHBY010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_148593"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1005)
                     /locus_tag="AC16HB_05895"
     CDS             complement(88..1005)
                     /locus_tag="AC16HB_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5606357.1"
                     /translation="MQKNPDRLPPLELLTAFEAAARHLSFTKAAAERFLTQSAISRQI
                     KALEDDLGVALFRRRHRALELTDTGHQLHEATRAALAELRRAVNRIRAPQRREVVALT
                     TTPGLASLWLIPRLSEFLRQHPGIDVRIDTSLDRRNLNAEGFDLAIRYARVGATAGAQ
                     LFREATLPVCSPALAKSHEQPLKKPADLRHHTLLQVTVPPGASVPLEWQPWLQAVGLG
                     DLEPAATLSFSSYDSAIAAALAGQGVALGRRPLIDHLLRTRKLVAPFKADPVASERAY
                     FLLIAPPARSRPALRALEEWLQAQAGSGA"
     gene            1146..1466
                     /locus_tag="AC16HB_05900"
     CDS             1146..1466
                     /locus_tag="AC16HB_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HIR5606358.1"
                     /translation="MSIELTCGQLRLNGHGIALLKNARGMRVAARHGVAWLTIDGVRD
                     DVVLNPGDAFRIDTDREVIVSAIAGPVEVAVQAGPTAPSAAPARRLWALLRGLFGGPH
                     TVAV"
     gene            1556..1939
                     /locus_tag="AC16HB_05905"
     CDS             1556..1939
                     /locus_tag="AC16HB_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR5606359.1"
                     /translation="MPLVTLTVRRPKSPQFKSQVLAAVHRALVSIGVPPADLFQRVLE
                     LDAQDFRFDPRYPDLASPRGDDFVLIEILLSVGRSVKVKRALLESLLADLAREPGIDA
                     EHVMVVFKETQWENWSFGGGRVLHA"
BASE COUNT          334 a          735 c          822 g          374 t
ORIGIN      
        1 tcggaggcgc cgtggtggct gtggcgccgc tacgggcgcg cttccgcggc cctcgggcgc
       61 aggcgttgat cccattcctc gcgaagatca agcgccggag ccggcctgcg cctgcagcca
      121 ctcctccagc gcgcgcagcg cggggcggct gcgcgcgggg ggcgcgatca ggaggaagta
      181 cgcgcgctcg gaggccaccg gatcggcctt gaacggcgcg accagcttgc gcgtgcgcag
      241 cagatggtcg atcagcggcc ggcggccaag ggccacacct tgccccgcga gcgcagcggc
      301 aatcgcgctg tcgtagctgc tgaacgagag tgtcgcggcc ggctccaggt cgccgaggcc
      361 gaccgcctgc agccacggct gccattcgag cggcacgctt gcgccgggcg gcaccgtgac
      421 ctgcagcagt gtgtggtggc gcaggtcggc gggtttcttc aggggctgct cgtgcgactt
      481 cgccagtgcc gggctgcaca cgggtagcgt cgcttcgcgg aagagctgcg cgcccgcggt
      541 agcgccgacg cgcgcgtagc ggatcgccag gtcgaagccc tcggcattca ggttgcgtcg
      601 gtcgaggctg gtgtcgatgc gcacatcgat gcccggatgc tggcgcagga attcggacag
      661 gcgcgggatc agccacagcg aagccaggcc cggcgtggtc gtcagcgcga ccacctcgcg
      721 ccgctgcggc gcgcggattc gattgaccgc gcgccgcagt tcggccagcg cggcgcgcgt
      781 ggcttcgtgc agttggtggc cagtgtcggt cagttcgagc gcgcggtggc gacggcggaa
      841 cagcgcgacg ccgaggtcgt cttcgagcgc cttgatctgc cggctgatcg ccgactgcgt
      901 gaggaagcgc tcggcggccg ccttggtgaa cgacaggtgt cgtgccgccg cttcgaaggc
      961 ggtcagcagt tccagcggcg gcaggcggtc cgggttcttt tgcatgcgat ctgcgaatgt
     1021 gaatcgtgcg aaaagatcgc ttgagcgcgg cttcgaccga ggcgcactat gcgcgcatcg
     1081 ctgcaacggg tcaaatgttt gcatgcattc caggcatgca gcgccgagac cgaaaggagt
     1141 ccgcgatgag catcgaactg acttgtggcc aactgaggtt gaacgggcac ggcattgcgc
     1201 tgctgaagaa tgcgcggggc atgcgcgtcg cggcgcggca cggcgtggcc tggctgacga
     1261 tcgacggcgt gcgtgacgac gtggtgctga acccgggcga cgcgtttcgg atcgacaccg
     1321 atcgcgaagt catcgtctcc gcaattgccg ggccggtcga agtcgccgtg caggctggac
     1381 cgacggcgcc ttctgcggcg ccggcacgac gcctgtgggc gttgttgcgg ggcttgttcg
     1441 gcgggccgca caccgtggcg gtctgaccgg atcatcgacg ggacgtgcgg cgatcgagtc
     1501 gccgccgccc gcttcgactg cagcacagat gacgcattga acgaaggagc atcggatgcc
     1561 actcgtcacg cttaccgtac gtcgaccgaa gtcgccgcag ttcaagtcac aggtgctggc
     1621 ggcggtgcac cgcgcgctgg tgtcgatcgg cgtgccgcca gcggatctct tccagcgggt
     1681 cctcgaactc gacgcgcagg acttccgttt cgacccgcgc tatcccgatc tggcgtcccc
     1741 gcgcggcgac gacttcgtgc tgatcgagat cctcttatcg gtcgggcgca gcgtgaaggt
     1801 caagcgcgcg ctgctcgagt cattgctcgc cgacctcgcg cgcgagcccg ggatcgatgc
     1861 ggaacacgtg atggtcgtgt tcaaggaaac ccagtgggag aactggtcct tcggcggcgg
     1921 acgcgtgctg cacgcatgac attctccgcg cggaccacgg gcctgcggtg gctgccggaa
     1981 cgcgtggcga tgcggctcca gatggacttg cgcccgatgg ctgccgcgtg cgcgagccaa
     2041 gcgtttacag gccctcgtcg ccccgaagtc agccttcgac gagggaccgg cccgcaggcg
     2101 acgggaagcc attcgccgaa tgcccgcagc ttccgctaac acggccgcct cgtggctctg
     2161 gccgtctgct atgcgggctg aacctcggtc acgccgaggt tcgccgccgc tgcagggccg
     2221 cgtagatgat caggcagctt tcgcttgccg tttgattcca accgc
//
LOCUS       DBNHBY010000237         4772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1366957, whole genome shotgun sequence.
ACCESSION   DBNHBY010000237 DBNHBY010000000
VERSION     DBNHBY010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1366957"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="AC16HB_05910"
     CDS             <1..563
                     /locus_tag="AC16HB_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5606360.1"
                     /translation="PDKENAVFRARIALKMRDDDPEYPALLLANYMFGGSGAGANRLW
                     NRIREKEGLSYSIGTGVNVDPFSQAGTIGFVAIAAPQNIDRVEQSTREELERTRRDGF
                     TADELDNARRGLLRDRALARAQDGALAGNWVRLLDAGRAFAYDRQLDERIGALTLDQV
                     NAAFRKYVVPERMVIVIGGDAAKGAK"
     gene            574..1029
                     /locus_tag="AC16HB_05915"
     CDS             574..1029
                     /locus_tag="AC16HB_05915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5606361.1"
                     /translation="MAYEDFRHRVPLRVRWAEVDMQGVVFNGHYLTYCDVCLTEYWRA
                     IGLRYPEDFLKLGSDTYARKITIQYHAPALYDDELDVCGRAARLGRTSLSFAIGIFRR
                     NEPAKVLIDAELVYVNVDPATKRPAPWPTPLREAMRGYERIAPIEAGER"
     gene            992..1462
                     /locus_tag="AC16HB_05920"
     CDS             992..1462
                     /locus_tag="AC16HB_05920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606362.1"
                     /translation="MNGSHRSRPANAELRIADVRIDLGDWERLRAEAVPIRRAVFVDE
                     QKVPSELEIDDWDLRSLHALARDRSGRALGTARLLPDGHIGRIAVLREARGAGVGTAL
                     VEAMLQAARTRGIGEAVLSAQTHALAFYRRLGFTEEGEPYDDAGIPHITMRRRL"
     gene            complement(1475..2308)
                     /locus_tag="AC16HB_05925"
     CDS             complement(1475..2308)
                     /locus_tag="AC16HB_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR5606363.1"
                     /translation="MCLAVFAWQSHPEFPLVVVANRDEFYARSTRAAAWWGQNVSLLG
                     GRDEEAGGAWFGINRRGRFSLLTNVRSPTERNPHAPSRGAVVLASLQSHEPIGEWLRD
                     LTPRAQVFNGFNLVVGDPLDQGRQPAELHYFSNRLTAETTLLRPGVYGLSNAFLDSPW
                     PKVTRTVSRFATMIAQRVSIDALLSMMADRSLALDRELPSTGVPLEWERALSAVLIRA
                     NGYGTRSTTVLTVRADGVANFVERTFDPASPDQHTDRAFEFTIGAGQRVSTGFGSPRG
                     K"
     gene            complement(2309..2593)
                     /locus_tag="AC16HB_05930"
     CDS             complement(2309..2593)
                     /locus_tag="AC16HB_05930"
                     /inference="COORDINATES: protein motif:HMM:NF027614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HIR5606364.1"
                     /translation="MQHWYVYYKLPRAQAAQIAARVRLMQDALAAAAPVRGRLLRRVD
                     DDGQTMTLMEQYDRIDDPADFESRLARAVAAAGLSAEIVAQRRVERFEDC"
     gene            complement(2617..3603)
                     /gene="ygfZ"
                     /locus_tag="AC16HB_05935"
     CDS             complement(2617..3603)
                     /gene="ygfZ"
                     /locus_tag="AC16HB_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR5606365.1"
                     /translation="MQAGTWLCPPEEVAACLKEPVVAALDELGVIAVEGADAMSFLHA
                     QLTNDVAGLDAERVQLNGYCSAKGRLYAVFDNWRDAQAVYLQLPREILPAVMKRLSMF
                     VLRAKAKLRDASSEWHALSIVGPGAAQALAGVAVAPPSGAARVLADGARLVRLPPAPQ
                     VEERFLLLARPDAANPLSVALGAMRRVPSAAFWWTQIDAAIPNVFAATQEKFVPQMIN
                     LEVLGGVNFRKGCFPGQEVVARSQYLGKLRRRMQVAHTDGVARAGADVCAEGVEQPIG
                     TVVMAASAPQGGMDALFECPVERAAGTLHLDGSSGPRLSPRPLPYALVDVTA"
     gene            3719..4720
                     /gene="mltG"
                     /locus_tag="AC16HB_05940"
     CDS             3719..4720
                     /gene="mltG"
                     /locus_tag="AC16HB_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5606366.1"
                     /translation="MRRLFVILLVVLLLAAAGALAWAARIYQQPLTMTGERVEFRIAP
                     GASARTIARQLKSAGIDIDETVFALAARFAKVSQSLRAGRYEILRGATLEQLLAKLRA
                     GDTLRERITLIEGWSLRDLRAALSAQPDLKQDSAVLSEAELLKAIGASESKAEGLFAP
                     DTYVFDAGTSDLDVLRQAYRAQAAVLQRAWESRAAGLPYATPYQALIMASIVEKKTGH
                     ATERTRIAGVFVNRLQRGMPLQTDPTVIYGLGEKFDGNLRKRDLITDGPYNTYTRAGL
                     PPTPIALAGRASIEAVLQPQQTRELYFVARGDGTSEFSETLNQHNRAVTKYQLGGKR"
BASE COUNT          788 a         1600 c         1644 g          740 t
ORIGIN      
        1 cgccggacaa ggagaacgcg gtgttccgcg cccgcatcgc gctgaagatg cgcgacgatg
       61 accccgagta tccggcactg ctgctcgcaa actatatgtt tggcgggtct ggcgcgggcg
      121 ccaaccggct gtggaaccgc atccgcgaga aggaaggctt gagctattcg atcggcacgg
      181 gtgtcaacgt cgatccgttc tcgcaggctg gcacgatcgg cttcgttgcg atcgcagcgc
      241 cgcagaatat cgaccgcgtc gagcagtcga ctcgagaaga gctcgagcgg acccgccgcg
      301 acggtttcac cgcggacgaa ctcgacaacg cacgccgcgg gctgttgcgc gaccgcgcgc
      361 tggcgcgagc ccaggacggc gcgctggccg gcaactgggt gcggctgctg gacgccggac
      421 gcgcgttcgc gtacgaccgc cagctcgacg agcgcatcgg cgcgctcacg ctcgaccagg
      481 tcaacgcggc gttccgcaaa tatgtcgtgc ccgaacgcat ggtgatcgtc atcggtggcg
      541 acgcggccaa gggcgcgaaa taggagaggg atcgtggcct acgaggattt ccggcaccgg
      601 gtgccgctgc gtgtgcgctg ggccgaggtc gatatgcagg gcgtggtatt caacggtcat
      661 tacctgacct actgcgacgt ctgcctgacc gaatactggc gcgcgatcgg actgcgctat
      721 ccggaggact tcctgaagct gggatccgat acctacgcgc gcaagatcac gatccagtat
      781 cacgcaccgg ccctgtacga cgacgaactg gatgtctgcg gccgcgcggc gcggctgggt
      841 cggacgagtc tgtccttcgc gatcggcatc ttccgtcgca acgagcccgc gaaagtgctg
      901 atcgacgccg aactcgtgta cgtgaacgtc gatccggcga ccaagcgccc ggcgccgtgg
      961 ccgacgccgc tgcgcgaggc gatgcgaggc tatgaacgga tcgcaccgat cgaggccggc
     1021 gaacgctgaa ctgcggatcg ccgacgtccg tatcgacctc ggcgattggg agcgcttgcg
     1081 tgccgaggcg gttccgattc gccgggccgt gttcgtcgac gagcagaagg ttccttccga
     1141 actggagatc gacgattggg atttgcgctc cctgcacgcg ttggcgcgcg atcgatcggg
     1201 gcgcgcgctc ggcacggcgc gcctgctgcc cgacgggcac atcggccgca ttgcggtgct
     1261 gcgcgaggca cgcggcgccg gtgtcggtac cgcactggtc gaggcgatgc tgcaggcggc
     1321 gcgcacacgc ggcatcgggg aggccgtgct gagtgcgcag acgcacgcgc tggcgttcta
     1381 tcggcgcttg ggcttcacgg aggagggcga accctacgac gacgccggca tcccgcacat
     1441 cacgatgcgg cgccggctgt agacgtgcgg gccgctactt gccgcgcggc gagccgaagc
     1501 cggtcgatac ccgctgaccg gcgccgatcg tgaactcgaa tgcgcgatcg gtatgctggt
     1561 cgggcgacgc gggatcgaac gtccgctcga cgaaattggc cacgccgtcc gcccgcacgg
     1621 tcagcaccgt ggtcgaacgc gtgccgtagc cgttggcgcg tatcagcact gccgacagcg
     1681 cacgttccca ttcgagcggc acgccggtcg atggcagttc acgatcgagt gcgagcgagc
     1741 ggtccgccat catcgagagc aacgcatcga tgctgaccct ctgcgcgatc atggtcgcga
     1801 agcggctcac ggtccgcgtg accttcggcc acggcgaatc gaggaacgca ttggacagcc
     1861 cgtatacgcc gggccgcagc agggtggtct cggccgtcag gcgattggag aaatagtgca
     1921 gttccgccgg ctggcggccc tggtcgagcg gatcgccgac caccagattg aagccgttga
     1981 acacctgcgc gcgcggcgtg aggtcgcgca gccactcgcc gatcggttcg tgcgactgca
     2041 gtgacgccag cacgaccgcg ccgcgcgacg gcgcatgcgg attgcgttcc gtcggcgagc
     2101 gcacattggt gagcagcgag aaccggccgc gccgattgat gccgaaccag gcgccgccgg
     2161 cctcttcgtc gcgtccgccg agcagcgaga cgttctgacc ccaccacgcc gccgcgcgcg
     2221 tgctgcgtgc gtagaactcg tcgcggttgg cgacgaccac gagcggaaac tcggggtgcg
     2281 actgccatgc gaacacggca aggcacattc agcaatcctc gaagcgttcg acgcgccgct
     2341 gcgcgacgat ctcggccgac agtcccgcgg ccgcgaccgc gcgtgcgagg cgggactcga
     2401 agtcggctgg atcgtcgatg cggtcgtact gttccatcag cgtcatcgtc tgcccgtcat
     2461 cgtccacccg gcgcagcagc cgtcctcgca cgggcgcggc cgcggcgagc gcgtcctgca
     2521 tcaggcgcac gcgcgccgcg atctgtgcgg cctgcgcacg tggcagcttg tagtagacgt
     2581 accagtgctg catttccatc cgtcattgcg acatcgctaa gcggtgacgt cgaccagagc
     2641 atacggcagc gggcgcggcg acagccgtgg gcccgacgaa ccgtcgaggt gcagtgtgcc
     2701 ggcggcgcgc tcgaccgggc attcgaacag cgcgtccatg ccgccctgcg gcgccgatgc
     2761 cgccatcacg accgtgccga tcggctgctc cacaccttcg gcgcacacat cggcgcccgc
     2821 ccgcgccacg ccgtccgtgt gcgcgacctg catccgccgt cgcagcttgc cgagatactg
     2881 gctgcgagca accacttcct ggccggggaa gcaacccttc cggaaattga cgccgccgag
     2941 cacctcgagg ttgatcatct gcggcacgaa cttctcctgc gtggccgcga agacgttcgg
     3001 gatcgcggcg tcgatctgcg tccaccagaa ggcggcgctc ggcactcttc tcattgcgcc
     3061 gagtgcgacc gacaaagggt tggctgcatc cggacgcgcg agcagcagga accgctcctc
     3121 gacctgcggt gcaggcggca ggcgaaccag gcgcgcgccg tcggccagca cccgcgcggc
     3181 gccactgggc ggtgcaaccg ccacgcccgc gagggcctgc gcagccccgg gtccgacgat
     3241 cgagagcgcg tgccattcgc tgctcgcgtc gcgcagcttc gccttggcgc gcagtacgaa
     3301 catcgacagg cgcttcatca ccgccggcag gatctcgcgc ggcagttgca ggtacacggc
     3361 ctgcgcatcg cgccagttgt cgaacaccgc gtacagccgg cccttggccg agcagtagcc
     3421 gttcagctgc acgcgctccg cgtcgagccc ggccacgtcg ttggtgagct gcgcatgcag
     3481 gaaactcatc gcgtccgcac cctcgaccgc gatcacgccg agttcgtcga gcgcggcgac
     3541 caccggctcc ttcagacatg cagcgacttc ctcgggcggg cacagccagg tgccggcttg
     3601 catcggggcg gacggatcgt tggccatggt gctcggactg cggtgatgcg ctcgtgttca
     3661 gctcgaatca aaaagtatca taggacggtc cgccgatcga ttcgccttca cgttgccgct
     3721 gcgccgcctg ttcgtgatcc tgctcgtcgt gctgctgttg gcggcggcgg gtgcattggc
     3781 gtgggcggcg cggatctacc agcagccgct gaccatgacc ggcgagcgcg tcgagttcag
     3841 gatcgcgccc ggtgcgtcgg cgcgcaccat tgcacgccag ctgaagtccg cgggcatcga
     3901 tatcgacgag accgtcttcg cgctggcggc gcgcttcgcg aaggtgtcgc agtcgctgcg
     3961 tgcgggacgc tacgagatct tgcgcggcgc cacgctcgaa cagctgctgg ccaagctgcg
     4021 cgcgggggac acgctgcgcg aacggatcac cctgatcgag gggtggtcgc tgcgcgacct
     4081 gcgtgccgcg ctgtcggcgc agccggacct gaagcaggac agcgcggtgc tgtccgaggc
     4141 ggagctgctg aaggcgatcg gcgcgagcga atcgaaagcc gagggactgt tcgcgccaga
     4201 cacctacgtg ttcgacgccg gcaccagcga tctcgacgtg ctgcgccagg cttatcgcgc
     4261 gcaggcagcg gtactacagc gcgcgtggga aagccgtgcg gcgggcctgc cgtacgcgac
     4321 gccgtaccag gcgctgatca tggcgtcgat cgtcgagaag aaaacgggac atgcgaccga
     4381 acgcacccgt atcgccggcg tgtttgtcaa tcgcctgcag cgcggcatgc cgctgcagac
     4441 cgaccctacg gtgatctacg gacttggcga gaagttcgat ggcaatctgc gcaagcggga
     4501 cctgatcacc gacgggccgt acaacacgta tacgcgcgcg ggactgccgc cgacgccgat
     4561 tgcgctggcc ggccgcgcgt ccatcgaggc ggtgctgcag ccacagcaaa cgcgcgagct
     4621 gtacttcgtc gcgcgtggcg acggcacctc ggagttttcc gagacactga atcagcacaa
     4681 tcgcgcggtg acgaagtacc agctcggcgg caaacgatga cctcgcgggg ctgcttcatc
     4741 acgtttgaag gcatcgacgg cgccggcaag ag
//
LOCUS       DBNHBY010000238         5513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_352235, whole genome shotgun sequence.
ACCESSION   DBNHBY010000238 DBNHBY010000000
VERSION     DBNHBY010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_352235"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1144)
                     /locus_tag="AC16HB_05945"
     CDS             complement(77..1144)
                     /locus_tag="AC16HB_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606367.1"
                     /translation="MNAPSDHGGSQHPALRGGLLALLAAAMFGVSTPLVQHFGRALGA
                     FTTAALLYAGAAAIGAVLRRPVEREARLQRGDALRLVAIAAFGAVLAPVALAWGLQRT
                     SATGASLMLALEALFTAILAWWLYREAIGARVWSALLFLLAGSAVLVLDQGRGGESGL
                     IGLLAVLAATAAWGMDNALSRTLAERDPARVVLAKAALGAAATFVLARLAAEPLPAPM
                     AAFGLLVVGATGYGLSLRFYLLAQRAFGAARTGSVFAAAPFIGAAAALALGDRSGTWA
                     TALGGALMLAGVVLHLTEAHGHAHEHGALEHEHAHRHDDGHHDHVHDPMPAGAHSHWH
                     RHAPLRHAHPHVPDAHHVHPH"
     gene            complement(1219..2730)
                     /locus_tag="AC16HB_05950"
     CDS             complement(1219..2730)
                     /locus_tag="AC16HB_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HIR5606368.1"
                     /translation="MANALTTSGAVQAGTPTTQKVGHGFSWKALVPVLVTVVLALIPA
                     PEGLAQHAWYFFAIFAGVIVGLMLEPLPGAAIGLIGVVLVTLLAPYVLFGPAELAKPG
                     FKAANASLTWALSGFSNTTVWLIFAAFMFALGYEKTGLGRRIALSLVKLMGKRTLTLG
                     YAVTFADVLLAPFTPSNTARSAGTVYPVIRNLPALYGSQPNDPSARRIGSYLMWVAIA
                     ATTITSSMFVTAMAPNLLAIEMVNKTAKIAISWTDWFVAFAPVGIVLLLALPLVVYFL
                     YPPQVKSGDEVPNWARGELTKLGGLTLREIELAVLVLLALALWIFGGDLINATTVAIA
                     VIALMVVLRVVSWDDILANKQAWNTLVWFGTLVALADGLARVGFVKWFADTVGAHMTG
                     FSPLLAMLVLVAVFFFTHYLFASITAHVTAMLPVMLAVGLGVPGMDMRVFALLLVLTL
                     GIMGILTPYATGPSPVYYGSGYIPTKDYWRLGAIFGVLYLVALLAIGLPWMAM"
     gene            complement(3009..4568)
                     /locus_tag="AC16HB_05955"
     CDS             complement(3009..4568)
                     /locus_tag="AC16HB_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5606369.1"
                     /translation="MRAGIEAAAAGSFRRALWLALVLAAGQVVLFGLAVGLTYSAPEI
                     DSAEQFVWSFSLENGYWKHPPMPSWIMHALLDVFGPSVVLPFIAAQACTVIALLLTWR
                     LACEFMTPNRALVAMALTSLVTYHNIGADSFNHSTALLPAQAAMVLFFYLAVRRGDRQ
                     FWLLAGLFAGLAILVKYTALLPIAALLLYFVTDRRLHTWRQTVGLALAGAVFALTLLP
                     HVLWLESTNFLPFRYAQAVAQAAPGTSALLLNVADFLLAQLVRSLPFALGLWLVLRRR
                     SAEAPAAPAQPLAKEDTHFLWMAGVGPLALTVLYSIATATELQSRWGSNAFLLVGVLA
                     LAAWPRAVTAETLRRTLWVVVIGQVALCLGQTLGKSVIAESLQRPTRANFPGELLARE
                     AQATWARHTERPLRLVVSDIWLGGNVVANSRHQRVAVLIDGMHFKSPWVRAGAVDACG
                     ALILDDLTPDKAGRDAPNPALDALMARADDTGIWTLPWAHASAADPATPRGLVRWGVI
                     LPRGSNECRLR"
     gene            complement(4780..5493)
                     /locus_tag="AC16HB_05960"
     CDS             complement(4780..5493)
                     /locus_tag="AC16HB_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HIR5606370.1"
                     /translation="MRFHILRYNPQKKGDAPRVQTYELEEADGMTLFIALNEIRDKQD
                     PSLSFDFVCRAGICGSCGMMINGRPGLACRTLTKNVGPDIHLAPLPVFELIADLSVNT
                     GKWMRAMSERLQTWVHMKMHEVDLARIEQKMDPELAEKIYELDRCIECGCCVAGCGTA
                     RMREDFVGAVGINKIARFRLDPRDARTDDDYYELIGDDDGVFGCMTLLACHDVCPKEL
                     PLATQIAFVRRKMVAQGLK"
BASE COUNT         1010 a         1868 c         1861 g          774 t
ORIGIN      
        1 gcgaaggcgc cgttgagcag gatcagtgcg agcagaaaga ggacttccat ggcgcgcttt
       61 cggtggactg cgagggtcag tgcggatgga cgtgatgcgc atccggcacg tgcggatgcg
      121 cgtggcgcag cggcgcgtgc cggtgccaat ggctgtgcgc gccggccggc atcggatcgt
      181 gcacgtgatc gtggtggccg tcgtcgtggc ggtgcgcgtg ttcgtgctcg agcgcaccgt
      241 gctcgtgcgc gtgcccgtgc gcctcggtca ggtgcagcac gacgccggcc agcattagtg
      301 cgccgccgag cgccgtcgcc cacgtgcccg aacgatcgcc cagcgccagt gcggcggccg
      361 caccgatgaa gggtgcggcc gcgaacaccg acccggtgcg cgcggcaccg aacgcgcgct
      421 gcgccagcag atagaagcgc aggctcaatc cgtagcccgt cgcgccgacg acgagcaatc
      481 cgaacgcagc catcggcgcc ggcaggggct cggcggcgag ccgcgccagc acgaacgtgg
      541 ccgcggcgcc gagcgcggcc ttggccagaa ccacgcgcgc cgggtcgcgc tcggctaacg
      601 tgcgcgagag cgcgttgtcc atgccccacg cggcggtggc cgccagcacc gcgagcaggc
      661 cgatcaaccc ggattcgccg ccgcgcccct ggtcgagcac cagcactgcg ctgccggcga
      721 gcagaaacaa cagcgccgac cacacgcgcg caccgatcgc ctcgcgatac agccaccacg
      781 cgagaatcgc ggtgaacagc gcctcgagcg cgagcatcag cgacgcgccg gtcgcactcg
      841 tgcgctgcag gccccacgcc agcgcaaccg gcgcgagcac cgcgccgaac gccgcgatcg
      901 cgacaagccg cagtgcgtcg cctcgttgca gccgtgcctc acgctcgaca ggccgccgga
      961 gcacggcacc gattgccgcc gcacccgcat acagcagcgc cgcggtcgtg aacgcgccga
     1021 gcgcgcgccc gaagtgctgc accagcggcg tgctgacgcc aaacatcgcc gcggcgagca
     1081 gcgctagcag tccgccgcgc agcgcggggt gctgcgatcc tccgtggtcg ctcggtgcgt
     1141 tcattcgaat gcccagtctg ccacgcagca tgaaaaacgg ggcgccgcag cgccccgttg
     1201 agtgttcagg ccacgaaact acatcgccat ccacggcaga ccgatcgcga gcagcgcgac
     1261 gaggtacagc acgccgaaga tcgcgcccag ccgccagtag tccttcgtcg gaatgtaacc
     1321 gctgccgtag tacaccgggc tcggtcccgt cgcatacggg gtgaggatgc ccatgatgcc
     1381 aagcgtcagc acgagcagca gcgcgaagac gcgcatgtcc atgcccggga cgcccaggcc
     1441 gaccgcgagc atgaccggca gcatcgcggt cacgtgcgcg gtgatgctgg cgaacaggta
     1501 gtgcgtgaag aagaacaccg cgaccagcac cagcatcgcg agcagcggcg agaagccggt
     1561 catgtgcgcg ccgacggtgt cggcgaacca cttgacgaag ccgacgcgcg ccaagccgtc
     1621 ggccagcgcg accagcgtgc cgaaccacac cagggtgttc cacgcctgct tgttggcaag
     1681 gatgtcgtcc cagctcacca cgcgcagcac gaccatcagc gcaatcacgg cgatcgcgac
     1741 cgtggtggca ttgatgaggt cgccgccgaa gatccacagc gccagcgcca gcagcaccag
     1801 caccgcgagt tcgatttcgc gcagcgtcag gccgccgagc ttcgtcagtt cgccgcgggc
     1861 ccagttcggc acctcgtcac cgctcttcac ctgcggcgga tagaggaagt agacgacgag
     1921 cggcagcgcc agcagcagca cgatgcccac cggcgcgaac gcgacgaacc agtcggtcca
     1981 tgagatcgcg atcttggcgg tcttgttgac catctcgatc gcgagcaggt tcggcgccat
     2041 cgcggtgacg aacatcgaac tcgtgatcgt ggtcgcggcg atcgcgaccc acatcaggta
     2101 gctgccgatg cggcgcgccg acgggtcgtt gggctggctg ccgtacagcg ccggcaggtt
     2161 gcggatcacc ggatacacgg tgcccgcgct gcgggccgtg ttcgacggcg tgaacggcgc
     2221 cagcagcacg tcggcgaacg tgaccgcgta gcccagcgtc agcgtgcgct tgcccatcag
     2281 cttcacgagc gacagcgcga tgcggcggcc gagcccggtc ttctcgtagc cgagcgcgaa
     2341 catgaacgcg gcaaagatca gccacaccgt cgtgttcgag aagcccgaca gggcccacgt
     2401 cagcgacgcg ttggcggcct tgaagccggg cttggcaagc tcggccggac cgaacaatac
     2461 gtacggcgcg agcagcgtga ccagcacgac gccgatcaga ccgatcgccg cgcccggcag
     2521 cggttcgagc atcaggccga cgatcacgcc ggcaaagatc gcgaagaagt accacgcgtg
     2581 ctgggcgagt ccttccggcg cgggaatcaa ggcaagaacg acggtgacga gcaccggcac
     2641 cagcgctttc cagctgaacc cgtgacccac tttctgggtg gtcggggtcc cggcttggac
     2701 agcgccactt gtggtgagcg cattggccat acgacctccg tagttaaggc aagaagaacc
     2761 gcttgaaatc cttcgggtgt cttcaatgcg gattctcggc gcgcccgcac gcaactttgt
     2821 gccgtaggtc acaaaattgg tcaggccatt tgaccgaatt ggtggatcgg gcggagattc
     2881 ggatgcggac gcaccaagag agggcgggcg gcgcgcctgc aaactgcagc gtcgatgcgc
     2941 gaggcttgct taacccaacg ccgtcggcga ccctgccgtc actcgatcgc gttatgcgct
     3001 gtgcattgtc agcgcagccg gcactcgttc gacccgcgcg gcaggatgac gccccagcgt
     3061 acaaggccgc gcggggtggc tgggtctgct gcggaggcgt gcgcccaggg caacgtccat
     3121 attccggtgt cgtcagcgcg cgccatcagg gcgtcgagag ccgggttcgg cgcgtcacgc
     3181 cccgccttgt cgggggtcag gtcatccagt atcagcgcgc cgcaggcatc gacggctccg
     3241 gcccggaccc acggcgattt gaaatgcatg ccgtcgatga ggaccgcgac ccgctgatgg
     3301 cggctgttcg caacaacgtt gccgccgagc cagatgtccg agacgaccaa ccgaaggggt
     3361 ctctcggtat ggcgcgccca ggtcgcctgc gcctctcgcg ccagcaattc acccggaaaa
     3421 ttggcgcgcg tggggcgctg aagactctcg gcaatcacgc tcttgccgag tgtctggcca
     3481 aggcagagcg caacttgccc gatcaccacg acccagagtg tccggcgcaa ggtctcggcg
     3541 gtcacagcac gcggccacgc cgccagcgca aggacgccga ccagcaggaa cgcattggat
     3601 ccccagcgag actgcagttc ggttgccgtc gcgatcgaat acagaacggt caacgcgagc
     3661 ggaccgacac cggccatcca gaggaagtgc gtgtcttcct tcgccaaggg ctgcgccggg
     3721 gcggcgggcg cctccgcgga gcgccgccgc aagacgagcc agaggccaag cgcaaagggc
     3781 agcgacctga ccagctgtgc cagcagaaag tcggcgacgt tgagcagaag cgcactcgta
     3841 ccgggcgcgg cctgcgctac tgcctgcgca tagcgaaacg gcagaaagtt ggtcgactcc
     3901 agccacagca cgtgcggcag aagcgtcaac gcaaacacgg cgccggcgag ggcgaggcca
     3961 actgtctgcc gccacgtatg caacctgcgg tcagtcacga aatacagcag cagcgcggct
     4021 atcggcagca acgccgtgta cttgacgaga atcgccaggc ccgcgaagag ccccgccagc
     4081 agccagaact gccggtcgcc gcgccgcact gccaggtaga agaaaagcac catcgcggcc
     4141 tgcgccggca gcaacgcggt gctgtggttg aaggagtcgg cgccgatgtt gtgataagtg
     4201 accagcgatg taagcgccat cgccaccagc gcgcgattgg gtgtcatgaa ctcgcaggca
     4261 aggcgccacg tgagcagaag cgcaatcacc gtgcaggcct gcgccgcaat gaacggcagc
     4321 acgaccgaag gcccgaacac gtccagcagc gcgtgcatga tccacgacgg catgggcggg
     4381 tgcttccagt agccgttctc gagcgagaac gaccacacga actgctcggc gctgtcgatt
     4441 tcgggcgcgc tataggtgag gccgaccgcg aggccgaaca gaacgacctg tcctgccgcg
     4501 agcaccaggg cgagccaaag cgcccggcga aagcttccgg cggccgccgc ttcgatgccg
     4561 gcgcgcacgc cattcgcagg gacggacacc gctgtgggta cgctggacat tggggcgcgc
     4621 ccgtgaatcg ccaaaaggcg cgatgttagc ggcacgggcg cgatgttcgt cctggcgaca
     4681 caagacttcc cgcgccggga cgcgaccccg atttggccgc caggattctg taagcgggcc
     4741 tcggggcgag aggtgcgctc gcgccccgcc gcgggccggt tacttcaagc cctgcgccac
     4801 catcttccgc cgcacgaaag cgatctgggt ggccagcggc agttccttcg ggcacacgtc
     4861 gtggcaggcg agcagcgtca tgcagccgaa cacgccgtcg tcgtcgccga tcagctcgta
     4921 gtagtcgtcg tcggtgcgcg cgtcgcgcgg atcgaggcgg aagcgcgcga tcttgttgat
     4981 gcccaccgca ccgacgaaat cctcgcgcat gcgcgcggtg ccgcagccgg ccacgcagca
     5041 gccgcattcg atgcagcggt ccagttcgta gatcttttcc gccagctcgg ggtccatctt
     5101 ctgctcgatg cgcgccaggt cgacctcgtg catcttcatg tgcacccagg tctgcagccg
     5161 ctcgctcatc gcgcgcatcc acttgccggt gttgaccgac aggtccgcga tcagctcgaa
     5221 caccggcagt ggcgccagat ggatgtcggg gccgacgttc ttcgtcagcg tgcggcaggc
     5281 caggccgggg cggccgttga tcatcatgcc gcagctgccg cagatgccgg cgcgacagac
     5341 gaagtcgaac gacagcgacg ggtcctgctt gtcgcggatc tcgttcagcg cgatgaacag
     5401 cgtcatgccg tcggcttcct cgagctcgta cgtctgcacg cgcggcgcgt cgcccttctt
     5461 ctgcgggttg tagcgcagga tgtggaaacg gatcttctgg ccggtggtga cag
//
LOCUS       DBNHBY010000239         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_690323, whole genome shotgun sequence.
ACCESSION   DBNHBY010000239 DBNHBY010000000
VERSION     DBNHBY010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_690323"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16HB_05965"
     CDS             <1..194
                     /locus_tag="AC16HB_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606371.1"
                     /translation="FDALLALAGEVATAEVDVEDDTTPAAIDAAWIDAPVTFLGAESP
                     CSTPRQPVEQAIHIHRRAA"
     gene            191..877
                     /gene="narI"
                     /locus_tag="AC16HB_05970"
     CDS             191..877
                     /gene="narI"
                     /locus_tag="AC16HB_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR5606372.1"
                     /translation="MTSFNFLLFGIYPYVCLAVFLIGSLIRFDREPYTWRSESSQMLR
                     TGQLRLGSNLFHIGILGIFFGHLFGLLTPIPVWHLLGVEAPAKQMLAIVAGGIFGFTM
                     LIGLIVLMHRRFTEPRIRATTAPMDWVVLWLLLIQLALGLFSLTVSWQHRDGGEMIRL
                     MTWAQHIVTLRTDAAAFVAEAAPVFKLHLLLGMTIFLVFPFSRLVHIWSGFGALAYLG
                     RAYQVVRTRR"
     gene            874..1512
                     /locus_tag="AC16HB_05975"
     CDS             874..1512
                     /locus_tag="AC16HB_05975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5606373.1"
                     /translation="MTVDLQAVRGQLLARAEALGITGESEEVIVDALLEREVRVEPPR
                     DDECRAYYAAHPQAFRAGTLVEADHILFAAREDSHRPALRDVAQEKLQALLHGEIEFA
                     DAAKQWSNCPSSAVGGNLGQLTREQVVPEFWQALEEFGATGLLPQPIETRFGLHIVRV
                     SRRVPGELLPYDMVADRVAMHLAEHRLRVALREYAHALLHDALGAESHAHVH"
     gene            1624..>1695
                     /locus_tag="AC16HB_05980"
     CDS             1624..>1695
                     /locus_tag="AC16HB_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5606374.1"
                     /translation="MGCSDSRIVPYALMDCGPGDLFMA"
BASE COUNT          248 a          583 c          557 g          307 t
ORIGIN      
        1 cattcgatgc tctgctggca ctggcgggcg aggtcgcaac cgccgaggtc gatgtggagg
       61 acgacaccac gcccgccgcg atcgacgccg cctggatcga tgcaccggtc acgttcctcg
      121 gcgccgagtc gccgtgcagc acgccgcgcc agccggtcga acaagcgatc cacatccatc
      181 ggagagccgc atgacttcgt tcaacttcct cctgttcggc atctatccgt acgtctgcct
      241 cgctgtcttt ctgatcggca gcctgatccg gttcgaccgc gagccgtata cgtggcgctc
      301 ggaatcgagc cagatgctgc gcaccgggca gctgcgcctg gggtcgaacc tgttccacat
      361 cggcatcctc ggcatcttct tcggccacct gttcggcctg ctgacgccga tccccgtctg
      421 gcacctgctc ggcgtcgagg cgccggccaa gcagatgctg gcgatcgtcg ccggcgggat
      481 cttcggcttc acgatgttga tcgggctcat cgtcctgatg caccgtcgat tcaccgagcc
      541 gcgcatccgc gccaccacgg cgccgatgga ctgggtcgtg ctttggctgc ttctgattca
      601 gctcgcgctc gggctgttct cgctcaccgt gtcgtggcag caccgcgacg gtggcgaaat
      661 gatccggctg atgacgtggg cgcagcacat cgtgacgttg cgcaccgatg ccgcggcgtt
      721 cgtcgccgag gcggcgccgg tgttcaagct gcacctcttg ctgggcatga cgatcttcct
      781 cgtgttcccg ttctcccgcc tcgtgcacat ctggagcggg ttcggcgcac tcgcctacct
      841 cggtcgcgcc tatcaggtcg tgcggacgcg gcgatgacgg tcgacctgca ggcggtccgc
      901 ggtcagctgc tcgcacgagc cgaagcgctc ggcatcaccg gcgagtcgga agaagtgatc
      961 gtggacgcgc tgctcgagcg cgaggtgcgc gtcgagccgc cgcgcgacga cgagtgccgc
     1021 gcttactatg ccgcgcatcc gcaggcgttc cgtgcgggca cgctggtcga ggccgaccac
     1081 atcctgtttg ccgcgcgcga ggattcgcac cgccccgcgc tgcgcgatgt cgcgcaggag
     1141 aagttgcagg cactgctgca cggcgagatc gaattcgccg acgccgcgaa gcagtggtcg
     1201 aactgtccgt ccagcgcagt cggcggcaac ctgggacagc tgacgcgcga gcaggtcgtg
     1261 cccgagttct ggcaggcgct ggaggagttc ggcgccacgg gcctcctgcc gcagccgatc
     1321 gagacgcgct tcggcctgca catcgtgcgc gtgtcacgcc gcgtgccggg cgaactgctg
     1381 ccgtacgaca tggtcgccga ccgcgtggcg atgcacctgg ccgaacaccg gttgcgcgtc
     1441 gcgctgcgcg aatacgcgca cgcgctgctg cacgacgccc tcggcgcgga gtcgcacgcc
     1501 cacgtgcact gaattgacga cctgctggcg cggctggcgc ggttcgagcg caatttcttc
     1561 ccgcgctacg agaaaaccta ccgccgcctc gtcgaataag gacagcaacc gaagacgctg
     1621 ttcatcggct gctccgattc acggatcgtg ccgtatgcgc tgatggactg tggtccgggc
     1681 gacctcttca tggcg
//
LOCUS       DBNHBY010000240         1726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_352387, whole genome shotgun sequence.
ACCESSION   DBNHBY010000240 DBNHBY010000000
VERSION     DBNHBY010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_352387"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC16HB_05985"
     CDS             <1..192
                     /locus_tag="AC16HB_05985"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957853.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5606375.1"
                     /translation="VGMGYSQHAGVVIVCDGTQEAARRIERVLWNDPGTGVMRHADAG
                     YELAVDCAREQGLNLPMAR"
     gene            189..>1726
                     /gene="hutH"
                     /locus_tag="AC16HB_05990"
     CDS             189..>1726
                     /gene="hutH"
                     /locus_tag="AC16HB_05990"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR5606376.1"
                     /translation="MSLTIVPGELGLAPLHAVHAGGVPLALDAGARARIRASAAVVQR
                     AAAGDTPVYGVNTGFGKLANQRISRTQLDTLQSNLIRSHSAGVGEPLPPAAVRLMLAL
                     KAASLARGHSGVCEQVVDTLLAVLNAGLVPYVPSQGSVGASGDLAPLAHMTLALMGEG
                     EMLADGRRAPAAALLRAAGIEPLTLEAKEGLALINGTQTSTALALHALLTFEPVLEAA
                     LVIGALTVDAARGSDGPFDPRIHALRGQPGQIDVAEYYRALLAGSEIRRSHLEGDDRV
                     QDPYSLRCQPQVVGACLDQLRHAALVLLREANAVTDNPLVFGEDASLISGGNFHAEQV
                     ALACDAMALAIAEVGAIAERRVAMLIDAGVSRLPPFLTADAGLNSGFMIAHVTAASLA
                     SENKSLAHPASVDSLPTSANQEDHVSMATFAARRLQPMIANTAHILGIELLAAAQGID
                     FLRPLQSSAALEQAHALLRESSPPVERDRYLAPDIERATALVTDGALSRVFRTLEGLP
                     ALWIP"
BASE COUNT          230 a          638 c          617 g          241 t
ORIGIN      
        1 gtcggcatgg gctactcgca gcacgccggc gtcgtgatcg tctgcgacgg cacgcaggaa
       61 gcggcccgcc gcatcgagcg cgtgctgtgg aacgatcccg gcaccggcgt gatgcggcac
      121 gccgacgcag ggtacgaact cgcggtcgat tgcgcgcgcg agcagggcct gaacctgccg
      181 atggcgcgat gagcctcacg atcgtccccg gcgaactcgg cctcgcaccg ctgcacgccg
      241 tgcacgcggg cggcgtgccg ctcgcgctcg acgcgggcgc gcgggcccgc atccgcgcca
      301 gtgccgccgt cgtgcagcgt gccgccgccg gcgacacgcc cgtctacggt gtcaacaccg
      361 gcttcggcaa gctcgccaac cagcgcatca gccgcacgca actcgacacg ctgcagtcga
      421 acctgatccg ctcgcacagc gccggcgtcg gtgagccgct gccgcccgcc gcggtgcgac
      481 tcatgctggc cctgaaggcg gcgagcctgg cgcgcgggca ctccggcgtg tgcgagcagg
      541 tggtcgacac tctgctcgcc gtgctcaacg ccgggctggt tccttacgtg ccgagccagg
      601 ggtcggtcgg cgcgtcgggc gatctcgcgc cgctggcgca catgacgctc gcgctgatgg
      661 gcgaaggcga gatgctggcc gacggccggc gcgcacccgc ggcggcgctg ctgcgcgcgg
      721 ccggcatcga accgctgacg ctggaagcca aggagggatt ggcgctgatc aacggtacgc
      781 agacgtcgac tgcgctcgcg ctgcatgcgc tgctcacgtt cgagccggtg ctcgaggccg
      841 cgctggtgat cggcgcgctg acggtcgacg cggcgcgcgg cagcgacggc ccgttcgatc
      901 cgcgcatcca cgcgctgcgc ggccagccgg ggcagatcga tgtcgccgag tactaccgcg
      961 cgctgctcgc cggcagcgag atccgccgct cgcacctcga gggcgacgac cgcgtgcagg
     1021 acccgtattc gctgcgctgc cagccgcagg tcgtcggcgc ctgcctcgac cagctgcgcc
     1081 atgcggcgct ggtgctgctg cgcgaagcca atgcggtgac cgacaatccg ctcgtcttcg
     1141 gcgaggacgc gagcctgatc tccggcggca acttccacgc cgagcaggtg gcgctcgcct
     1201 gcgatgcgat ggcgctggcg atcgccgagg tcggtgccat cgccgagcgg cgggtcgcga
     1261 tgctgatcga cgccggcgtg tcgcgcctgc cgccgttcct gaccgcggac gcgggactga
     1321 attcgggctt catgatcgcg catgtgaccg cggcgtcgct ggcgagcgag aacaagtcgc
     1381 tggcgcaccc ggccagcgtc gattcgctgc cgacctcggc caaccaggaa gatcacgtgt
     1441 cgatggcgac tttcgccgcg cgccgcctgc agccgatgat cgccaacacc gcgcacatcc
     1501 tcggcatcga gctgctcgcc gccgcgcagg gcatcgattt cctgcggccg ctgcagagct
     1561 cggccgcgct ggagcaggcg cacgcgctgc tgcgcgagtc cagcccgccg gtggagcgcg
     1621 accgctacct cgcgcccgac atcgagcgtg ccacggcgct cgtcaccgac ggcgcgctgt
     1681 cgcgcgtgtt ccgcaccctc gagggcctgc cggccctgtg gatccc
//
LOCUS       DBNHBY010000241         3664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_690669, whole genome shotgun sequence.
ACCESSION   DBNHBY010000241 DBNHBY010000000
VERSION     DBNHBY010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_690669"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="AC16HB_05995"
     CDS             complement(<1..584)
                     /locus_tag="AC16HB_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606377.1"
                     /translation="MDINVFPRHELPTVFRALRTALGPTSALDARERAFLQTYARITG
                     FDLGESDPLPIEARHVSIDGAHARKRLVQLSALAVLLSRPVKPASQAFLKALARHLAT
                     HDPVIDVIDALIKGRRRKVRLLAMRRAMRVMFKEAHLAEGVLGTVRLASALMFKAAVN
                     RVLMVKYKRLGMLPEGTLGREYWKHMTREGFNFPG"
     gene            complement(647..1996)
                     /locus_tag="AC16HB_06000"
     CDS             complement(647..1996)
                     /locus_tag="AC16HB_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR5606378.1"
                     /translation="MNTNLLKLALAAQFTLLSGPSQAIDLLGSYEKALRFDPSMQAAG
                     EAVLAGREKSLQGDALLRPQVSLAASYSHVDDRTTTSLPPELAAVIKPESSGNVHQAA
                     LQLKQTLYDAKAAADRRQLHQQTQLAEIRFGDARQDLMQRVAEAYFQVLTAQENLRVV
                     LAEKAAVGLQRDRAQARFEVGRGRITDVQEAQARYDSVLTKEVAATSTLALRQAQYEE
                     LTGTRAEALAELRPGFTPAPPQPDSLQAWQQKSRDYNTRVQTKRSELAIATAEIGKHK
                     LSGRPTLDLVASYAHKGSNGGLSPTIAPDSNRSAVIGVQLNIPLFAGGALDSRERESL
                     AKQRQAEHELSAAQRDARLQVQDAYLAVKTGVARIVSVEQSVLSAQTALEATTLGRDV
                     GNRTELDVLDAQQRLFAAQLDLAQARNDYLLGRVRLAAAAGELKESDLRSLNAYLAN"
     gene            complement(2006..>3664)
                     /locus_tag="AC16HB_06005"
     CDS             complement(2006..>3664)
                     /locus_tag="AC16HB_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5606379.1"
                     /translation="VVGVSLMLSWIVAVLFTPYIGYKLLREDHAASHDEDAVYQRGFY
                     VRFRRVVDACLVHRKWVIGVTVAAFVGAIALFRLVPQQFFPASDRPELMIDLWLPQAA
                     TFDASLREVQALEAKLKDDPDVVAVTSYVGNGSPRFYLPLDVQTPNLNLGEMMVMTKG
                     GAARERMLAKIHNLFDTDFPFVRGRVNRLANGPPVGYPVQFRVFGQDNAKVQAIADQV
                     AGIMRADPHLRRVNQDWGERLKRVRVDVDQDKARAVGITSREIKIALENSLSGTNVTQ
                     YREGDDDIDVVGRLVASERTDLNNLKDAQIFLRNGKFVPVSQVARLSLDSEDSVLWRR
                     NRVPTLTVRADVAGAEAPDVTRALKPKLDALARTLPLGYGIEVGGAQESSSKAQASII
                     AVMPLTIVAVLLLLMVQLQDMKKMALVLLTAPLGLIGVSAILATFRIPFGFVAMLGVI
                     ALAGMIIRNSVILVVQIDHDLAAGRPLWHAIVESTVRRLRPIVLTALAAILAMIPLTR
                     SVFWGPMAWAIMGGLFVATLLTLLFLPALYASWYRAQKPVAMPA"
BASE COUNT          575 a         1241 c         1212 g          636 t
ORIGIN      
        1 ccggggaagt tgaatccctc ccgggtcatg tgcttccagt attcgcgccc gagagttcct
       61 tccggcagca tgcccaggcg cttgtacttc accataagca cccggttcac cgctgccttg
      121 aacatcaatg cgctggccag gcggaccgtg cccaacacgc cttcagcgag gtgcgcctcc
      181 ttgaacatca cgcgcatcgc gcgtcgcatc gcgagcaggc ggaccttgcg gcgccgcccc
      241 ttgatcaacg cgtcgatcac gtcgatcacg gggtcgtgag tcgcgaggtg gcgcgcgagc
      301 gccttcagga acgcctgcga ggcgggcttc acgggacggc tgagcagaac ggcaagcgct
      361 gacaactgga ccagccgctt gcgcgcgtgg gccccgtcga tcgagacgtg gcgtgcctcg
      421 atcggcagcg ggtcggattc ccccaggtca aagccggtga tccgggcgta ggtctggagg
      481 aacgcacgct cgcgtgcgtc gagtgcactc gtggggccga gcgccgtgcg cagcgcgcgg
      541 aagaccgtcg gcaattcatg acggggaaaa acgttgatgt ccatgggaat tccgtttggt
      601 ttgaggttcg aaaagtcgtc gagcgttgat gaccggaggg gcccgctcag ttggcgaggt
      661 aggcattgag cgaccgcagg tcgctctctt tcagttcacc ggctgccgcc gcgaggcgga
      721 cccggccgag caggtagtcg ttgcgcgcct gcgcgaggtc gagctgcgcc gcgaacaggc
      781 gttgctgcgc gtcgagcacg tcgagttcgg tgcggttgcc gacatcgcgg ccgagcgtgg
      841 tcgcttcgag cgcggtctgg gcggacagca ccgactgctc gaccgatacg atgcgcgcca
      901 cgccggtctt caccgcgagg taggcgtcct gcacctgcag tcgcgcgtcg cgctgcgcgg
      961 cgctcaactc gtgctcggcc tggcgctgct tcgccagcga ctcgcgctcg cgcgagtcga
     1021 gcgcgccacc ggcgaacagc gggatgttca actgcacccc gatcaccgca gagcggttgc
     1081 tgtcgggtgc gatcgtcggc gacaggccgc cgttactgcc cttgtgcgca tagctggcca
     1141 cgaggtccag cgtgggccgg ccgctcagtt tgtgcttgcc gatctcggcg gtcgcgatcg
     1201 ccagttcgct gcgctttgtc tgcacgcgcg tgttgtagtc cctgctcttc tgttgccacg
     1261 cttgcaggct gtcgggttgg ggcggtgcgg gcgtgaagcc cggtcgcaac tcggccagcg
     1321 cttcggcccg ggtgccggtc agctcctcgt actgcgcctg ccgcagcgcg agcgtgctcg
     1381 tcgctgcgac ctccttcgtg agcacgctgt cgtagcgcgc ctgtgcctcc tgcacgtcgg
     1441 tgatcctgcc gcggcccacc tcgaagcgag cctgcgcgcg gtcgcgctgc aagccgaccg
     1501 ccgctttctc ggccagcacc acgcgcaggt tctcctgtgc cgtcagcacc tggaaatagg
     1561 cttcggcgac gcgctgcatc aggtcctgcc gcgcgtcccc gaacctgatc tccgcgagct
     1621 gcgtctgctg atgcagttgc ctcctgtcgg ccgccgcctt ggcgtcatac agggtttgct
     1681 tcagctgcag cgccgcctga tgcacgttgc ccgaactttc cggcttgatc accgccgcca
     1741 gttccggcgg caggctggtc gtcgtgcggt cgtcgacgtg cgaatagctt gcggccagcg
     1801 agacctgtgg ccgcagcaat gcatcgccct gcagcgactt ctcgcgacct gcgagcacag
     1861 cctcgccggc ggcctgcatg ctcgggtcga accgcagcgc cttctcgtac gagccgagca
     1921 gatcgatcgc ctggctgggg ccagacagca aagtgaactg cgcggcgagc gccagtttca
     1981 gcagattcgt gttcatcgtc atcctctacg ccggcatggc aacgggtttc tgcgcgcggt
     2041 accagctcgc gtacagcgcg ggcaggaaga gcagcgtcag cagggtcgcg acgaacagac
     2101 cgcccatgat cgcccaggcc atcgggcccc agaacaccga acgggtcagc ggaatcatcg
     2161 cgaggattgc ggccagtgcg gtcagcacga tcggccgcag ccgccgcacc gtcgactcga
     2221 cgatcgcgtg ccacaacggc cgaccggccg ccagatcgtg gtcgatctgc accaccagga
     2281 tcaccgagtt gcggatgatc attccggcaa gcgcgatcac gcccagcatc gcgacgaagc
     2341 cgaacggaat gcggaaggtc gccaggatcg cggaaacgcc gatcaggccg agcggtgcgg
     2401 tcaggaggac cagcgccatc ttcttcatgt cctgcaactg caccatcagc agcaggagca
     2461 cggcgacgat ggtcagcggc atcacggcaa tgatcgatgc ctgtgccttg ctgctcgact
     2521 cctgtgcgcc gcccacttcg atgccgtagc cgagcggcag cgtccgtgcc agcgcgtcga
     2581 gcttgggctt cagtgcgcgc gtcacgtcgg gtgcttcggc gccagcgaca tcggcgcgca
     2641 cggtcagcgt cggcacgcga ttgcgccgcc acagcacgct gtcctcgcta tcgagcgaca
     2701 gccgcgccac ctgcgatacc ggcacgaact tgccgttgcg caaaaagatc tgcgcgtcct
     2761 tcaggttgtt caggtccgta cgctcggatg cgaccagacg cccgaccacg tcgatgtcgt
     2821 cgtcgccttc gcggtactgc gtgacgttcg tgcccgacag cgagttctcc agcgcgatct
     2881 tgatctcgcg cgacgtgatg cccaccgcgc gcgccttgtc ctggtccacg tcgacgcgca
     2941 cgcgcttcag ccgctcgccc cagtcctgat tgacgcggcg cagatgcggg tcggcgcgca
     3001 tgatcccggc cacctggtcg gcgatcgcct gcactttggc gttgtcctga ccgaacacgc
     3061 ggaactgcac cgggtagccg accggtgggc cgtttgcgag ccggttgacg cggccgcgca
     3121 cgaatggaaa gtcggtgtcg aagaggttat ggatcttcgc cagcatgcgc tcgcgcgccg
     3181 cgccgccctt ggtcatcacc atcatttcgc cgaggttgag gttcggcgtc tgcacgtcga
     3241 gcggcaggta gaagcgcggg ctgccgttgc cgacgtagct ggtgaccgcc accacgtcgg
     3301 gatcgtcctt cagcttcgct tcaagagcct gcacctcgcg caggcttgcg tcgaacgttg
     3361 ccgcctgcgg cagccacagg tcgatcatca gttcggggcg gtcggaagcc gggaagaact
     3421 gctgcggcac caggcggaac agcgcgatcg cgccgacgaa tgccgcgacc gtgacgccaa
     3481 tcacccattt gcgatgcacg aggcacgcat cgacgacgcg ccggaaccgc acgtagaagc
     3541 cgcgctggta gacggcgtcc tcgtcgtggc ttgccgcgtg gtcttcgcgc agcagcttgt
     3601 agccgatgta cggcgtgaac agcaccgcga cgatccacga cagcatcagc gaaacgccga
     3661 ccac
//
LOCUS       DBNHBY010000242         1622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_284867, whole genome shotgun sequence.
ACCESSION   DBNHBY010000242 DBNHBY010000000
VERSION     DBNHBY010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_284867"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16HB_06010"
     CDS             <1..360
                     /locus_tag="AC16HB_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5606380.1"
                     /translation="RVRQPFNVNTLAQAAAVAALGDADFLRTSYELNLRGMQQLVAGF
                     EALGLEHVPSFGNFVLVKVGPAAKVYQALLRRGVIVRPVANYGLPEWLRVSIGLPEEN
                     AKLLAALPEALASARAG"
     gene            357..1235
                     /locus_tag="AC16HB_06015"
     CDS             357..1235
                     /locus_tag="AC16HB_06015"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR5606381.1"
                     /translation="MKLALIGVGLIGGSAAVAWRRAGAISEVVGFDRDSVTLQRAQAL
                     GVIDRAAPSAGDAVHDADLVLLAVPVGAMRATFAAIAATVPAHALITDVGSTKRSVID
                     AARAELNATSSVSSRRFVPGHPIAGREQPGVEHADAQLFEGRLFVSTPVAETDASALT
                     RIEALWTAAGAKVSRMDADEHDRIFAAVSHLPHLLAFALVARIASEADGERKLGFAGA
                     GFRDFTRIAASSPEMWRDISVANRAALGDELRGYRALLDDLQRAVDAGDAAAIERVFD
                     LASRTRRRHARTFDAE"
     gene            1225..>1622
                     /locus_tag="AC16HB_06020"
     CDS             1225..>1622
                     /locus_tag="AC16HB_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR5606382.1"
                     /translation="MPSDASWPAVLTIAPIARASGAVPLPGSKSISTRALLLAALALG
                     ETELTGLLDADDTRVMIAALRALGVRLEQSAGAVRVTGCAGAFPVREADLFLGNAGTA
                     MRPLTAALAFAGGRYRLDGVARMRERPIGDL"
BASE COUNT          216 a          555 c          603 g          248 t
ORIGIN      
        1 cgcgtgcggc agccgttcaa cgtcaacacg ctggcgcagg cggcggccgt ggccgcgctc
       61 ggtgacgcgg acttcctgcg cacgagctac gagctgaatc tgcgcggcat gcagcagctg
      121 gtcgcgggct tcgaagccct gggactggag cacgtgccga gcttcggtaa cttcgtgctg
      181 gtcaaggtcg ggccggcggc gaaggtctat caggcgcttc tcagacgcgg cgtgatcgtg
      241 cgcccggtgg ccaattatgg cctgcccgag tggctgcgcg tcagcatcgg tctgccggag
      301 gagaacgcga agctcctcgc cgcgttgcct gaggcgctgg cgtccgcgcg cgcgggatga
      361 agctggcgct gatcggcgtc ggcctgatcg ggggctcggc cgcggtcgcg tggcgacgcg
      421 ccggcgccat ctccgaggtg gtcggcttcg atcgcgacag tgtcacgctg caacgggcgc
      481 aggcgctcgg cgtgatcgac cgcgcggcac cgagcgcggg cgatgcggtg cacgatgccg
      541 atctcgtgct gctcgccgtg ccggtgggcg cgatgcgcgc aacgttcgcc gcgatcgccg
      601 cgacggttcc cgcgcatgcg ctgatcaccg atgtcggcag caccaagcgc tcggtgatcg
      661 acgccgcacg cgcggaactg aacgcgacga gcagcgtctc ctcccgccgc ttcgtccccg
      721 ggcacccgat cgccggacgc gagcagccgg gcgtggaaca cgccgacgcg cagcttttcg
      781 aaggcaggct cttcgtcagc acgccggtcg cggagacgga cgccagtgcg ctgacgcgca
      841 tcgaggcgct gtggacggcg gcgggcgcca aggtctcgcg catggacgcc gacgagcacg
      901 atcgcatctt tgccgcggtc agccatctgc cgcacctgct cgcgttcgcg ctggtcgcgc
      961 gcatcgcgtc ggaagcagac ggcgagcgca agctcggctt tgccggtgcg ggcttccgcg
     1021 acttcacgcg tatcgccgcc agcagcccgg agatgtggcg cgacatctcg gtcgccaatc
     1081 gcgctgcgct cggcgacgaa ctgcgcggct accgcgcact gctcgacgac ctgcagcggg
     1141 ccgtcgacgc gggcgacgcg gccgcaatcg agcgcgtgtt cgatctggcg agccgcacgc
     1201 gccgtcgcca cgcgaggacc ttcgatgccg agtgatgcga gctggcccgc tgtgttgacg
     1261 atcgcgccga tcgcacgggc cagcggcgcg gtgccgctgc cgggctcgaa gagcatttcc
     1321 acccgtgcgc tgctgctggc ggcactcgcg ctgggcgaga cggagctgac gggcctgctc
     1381 gacgccgacg atacgcgcgt gatgatcgct gcgctgcgcg cactcggtgt acgactcgag
     1441 caaagcgcgg gtgcggtgcg cgtgaccggt tgcgcgggcg cgtttccggt ccgggaggcg
     1501 gatctctttc tgggtaacgc cggcaccgca atgcgcccgc tgacggcagc actcgcgttt
     1561 gccggtggcc gctatcggct cgacggcgtg gcgcggatgc gcgagcggcc catcggcgat
     1621 ct
//
LOCUS       DBNHBY010000243         2625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1097747, whole genome shotgun sequence.
ACCESSION   DBNHBY010000243 DBNHBY010000000
VERSION     DBNHBY010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1097747"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="AC16HB_06025"
     CDS             <1..1623
                     /locus_tag="AC16HB_06025"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606383.1"
                     /translation="LADLPERRMREFRGAKVSMIFQEPATSLNPVLTVGRQIVESIET
                     HTPLRGDAARAKAVEWLRRVGLPEPERRVDAYPFQLSGGQKQRVMIAIALAVEPDLVI
                     ADEPTTALDVTIQKQILDLLKELQAAQRMSLLLITHDLGIVAEMAHRIALMYAGQVVE
                     IAGTAEFFARPLHPYAQLLFEAIPDTGKRGRQLAAIPGTVPPLNRPFTACRFVERCPA
                     AMERCRATPPPLFEPRAAHHVRCVLHESGPGSLLPRVHSEAIAVRETRPEPTPASPLL
                     EVRDYRVWFPIRRGLLKRTVGYVKAVDGVSFALAPARTLALVGESGCGKTTTGKALLQ
                     LLRGVAHVLGDARLEGRPLDRLHGETLRKARRDIQIIFQDPFASLNPRMRVAEILEEG
                     VGSLRPEIGAGERRAHIAALLEKVGLRADSMQRYPHEFSGGQRQRIAIARALAVEPKL
                     IVCDEPTSALDVSVQAQILNLLAQLQRELGVAYLFITHNFGVVEYLAHDIAVMKNGRI
                     VEAGAAEQVLERPAHEYTKTLLAAVPRLKRAA"
     gene            complement(1657..2001)
                     /locus_tag="AC16HB_06030"
     CDS             complement(1657..2001)
                     /locus_tag="AC16HB_06030"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5606384.1"
                     /translation="MGSVLTPLEVRLTGQLAERAHAMPSALRVWVFGSRARGASTPDS
                     DLDVAVEFDAPETPELRSWLERIRIETEAPLVDRWPGFVNLVGLYSDDPDSRLARCVR
                     AEGVLIWERRFD"
     gene            complement(2017..2436)
                     /locus_tag="AC16HB_06035"
     CDS             complement(2017..2436)
                     /locus_tag="AC16HB_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5606385.1"
                     /translation="MTSDDARLLLAQRELDEATREAAAADLLTGQDFFRQAVTRAYFA
                     AFHVAQALLATHGIEAATQEGVQRLLGLHFVLPGALSKDAGRALSALSARRHEADYRL
                     LVDVDGKTWESARSEAMIFVAAARDYLKRAWPTLGLH"
     gene            2477..2625
                     /locus_tag="AC16HB_06040"
                     /pseudo
     CDS             2477..2625
                     /locus_tag="AC16HB_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568003.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          445 a          903 c          884 g          393 t
ORIGIN      
        1 ctggcggacc tgccggagcg gcgcatgcgc gaattccgcg gcgccaaggt gtcgatgatc
       61 tttcaggagc cggccacgag cctcaatccg gtgctgaccg tggggcgcca gatcgtcgag
      121 tcgatcgaaa cgcatacgcc gctgcgcggt gacgccgcgc gagccaaggc ggtcgagtgg
      181 ctccggcgcg tcggactgcc cgaaccggag cggcgcgtcg atgcgtatcc gttccagctc
      241 tccggcgggc agaagcagcg cgtgatgatc gcgatcgcgc tggcggtcga accggatctg
      301 gtgatcgccg acgaaccgac caccgcgctc gacgtgacga tccaaaagca gatcctcgac
      361 ctgctcaagg aactgcaggc ggcgcagcgg atgtcgctgc tgctgatcac gcacgatctg
      421 ggcatcgtcg ccgagatggc gcaccggatc gcgctgatgt acgcgggcca ggtggtcgag
      481 atcgccggta cggccgagtt cttcgcgcgc cccctgcacc cgtacgcaca actgctgttc
      541 gaggcgattc ccgacaccgg caagcgcgga aggcagcttg ccgcgatccc cggcacggtg
      601 ccgccgctca atagaccgtt caccgcatgc cgctttgtcg agcgctgccc ggccgcgatg
      661 gaaagatgcc gcgcgacgcc tccgccgttg ttcgagccac gggcggcgca ccacgttcgc
      721 tgcgtattgc acgaaagcgg gccgggcagc ctccttccgc gcgttcattc cgaggcgatc
      781 gccgttcggg agacgcgccc ggagccgact cccgccagcc cgctgctcga agtccgtgac
      841 taccgcgtct ggtttccgat ccggcgcggc ctgctgaagc gcacggtcgg ctacgtgaag
      901 gcggtcgacg gcgtgtcgtt cgcgctcgcg ccggcgcgca cgctggcgct ggtcggcgag
      961 tccggctgcg gcaagacgac gacgggcaag gcgctgctgc agttgctgcg cggcgtggcg
     1021 catgtgctgg gcgatgcgcg actcgaaggg cggccgctcg atcgtttgca cggcgagacg
     1081 ctgcgcaagg cgcggcgcga catccagatc atcttccagg acccgttcgc ctcgctgaac
     1141 ccacggatgc gcgtggccga gatcctggag gagggggtcg ggtcgctgcg accggagatc
     1201 ggcgcgggtg aacgccgcgc gcacatcgcg gcgctgctgg agaaggtcgg cctgcgcgcg
     1261 gacagcatgc agcgctaccc gcacgagttc tccggcggcc agcgtcagcg gatcgcgatc
     1321 gcgcgcgcgc tggcggtgga gccgaagctg atcgtctgcg acgagccgac ctcggcgctc
     1381 gacgtatcgg tgcaggcgca gatcctgaac ctgctggccc aactgcagcg cgaactcggc
     1441 gtcgcgtatc tcttcatcac gcacaacttc ggcgtggtcg aatacctcgc gcacgacatc
     1501 gcggtgatga agaacggccg catcgtcgag gccggcgctg ccgaacaggt gctcgagcgg
     1561 ccggcgcacg agtacacgaa gacgctgctg gccgcggtgc cgcggctgaa acgggcggcg
     1621 taaagcggcg cctgctcaga cggagcttcg gccaggctaa tcgaagcgcc gctcccagat
     1681 caacacccct tcggctcgga cgcaacgcgc gagccgcgag tccgggtcgt ccgaatacaa
     1741 cccgaccaga ttgacaaacc caggccatcg atcgaccagc ggcgcctcgg tttcaattcg
     1801 aattctttcc agccaggagc gaagctcagg cgtttcgggg gcgtcgaact cgaccgcaac
     1861 atcgagatcg gaatccggcg tcgatgcccc gcgcgcacgg cttccgaaca cccagacgcg
     1921 cagcgcgctc ggcattgcgt gtgcccgctc cgccaactgc cccgtgaggc gaacttccag
     1981 tggcgtcagt accgatccca tcagatgccc ggcgtttcaa tgcaggccca gggtcggcca
     2041 cgcacgcttc aggtaatcgc gcgccgctgc gacgaaaatc atcgcctcgc tccgcgccga
     2101 ttcccacgtc ttcccatcga catcgaccaa caggcggtaa tcggcctcat gtcggcgcgc
     2161 cgacagtgcg gaaagcgccc gccctgcatc cttcgaaagc gcgccgggca gtacgaagtg
     2221 cagtccgagc aaacgctgca cgccctcctg cgtggccgcc tcaatgccgt gagtcgccag
     2281 cagggcctgt gcgacatgga acgcggcgaa atacgcgcgg gtcaccgcct ggcggaagaa
     2341 atcctgaccc gtcagcagat ccgccgccgc ggcttcgcgc gtcgcctcgt cgagttcccg
     2401 ctgtgcgagc aacaaccgcg cgtcatcgga agtcatatcg ggtcggtacc gaatgggccg
     2461 cagagcagat tacgccgcgc acatcgcggc gctgctggag aaggtcggcc tgcgcgcgga
     2521 cagcatgcag cgctaccggt acgagttctc cggcgaccag cgtcagcgca tcgcgatcgc
     2581 gcgctggcgg cggagccgaa gctgatcgtc tgcgacgagc cgact
//
LOCUS       DBNHBY010000244         2323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_353178, whole genome shotgun sequence.
ACCESSION   DBNHBY010000244 DBNHBY010000000
VERSION     DBNHBY010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_353178"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="AC16HB_06045"
     CDS             <1..718
                     /locus_tag="AC16HB_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606386.1"
                     /translation="MHRRLARDMQIIFQDPYASLNPRMTVGAIIGEALIIHKLARTRR
                     EFEDRIVQLLETVGLAADHMRRYPHEFSGGQRQRIGIARALAVSPKLIVCDEAVSALD
                     VSIQAQVINLLEDLRSQFNLTYIFIAHDLSVVEHISQRVAVMYLGRIVEIAPARSLYT
                     NPKHPYTEALLAAVPIPDPTVKRKRIPLQGDVPSPLNPPSGCPFHTRCPIAQQPLCAT
                     QTPPLKQTGAGHWVACHLRG"
     gene            723..1259
                     /locus_tag="AC16HB_06050"
     CDS             723..1259
                     /locus_tag="AC16HB_06050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5606387.1"
                     /translation="MNTALLVIDVQQGLCEGESPAFGSQQVIERINRVTDQARAAGAS
                     VIFVQHESASELEFGTDAWRLARGLHVQPGDLRLRKTTCDSFHRTELQETLQRHAVTR
                     LVICGMHTEFCVDTTVRRALALGYEVVLVADAHTTADRPHLAAAQIIRHHNETLANIE
                     SFGVRVRAIPHGELTFGA"
     gene            complement(1302..>2323)
                     /locus_tag="AC16HB_06055"
     CDS             complement(1302..>2323)
                     /locus_tag="AC16HB_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606388.1"
                     /translation="MVASAATERGTGAVTFETWREPNEGAYTIDVPKGWRISGGVRRF
                     SPLDVRSALSAVSPDGSIRIFIGDYDLVPRREPDAATQMAGWREGQMVAGGLIARYMT
                     GVQFAERYPAWKLCRQPRIAQSGILRRESQTLSAEIARFGQGTGLASSASVGEAIFRC
                     PEGEGFVLATTLLVRPAYGQGVSGWFVYQLSGFITRDPRQGYFAKYVLSAMLASQKMN
                     PQWEMRVAQAGGQYANSMMQMSNAITRTTIQHARQQAAQGSAGGWNHPNTANLPKITR
                     DPGVEARRDIANRGSRRVCDDLGTCKTVDNAWSTVWRDHNGNVVPGSASGYPPDYSGQ
                     WTEMK"
BASE COUNT          371 a          823 c          769 g          360 t
ORIGIN      
        1 catgcatcgc cgcctggcgc gcgacatgca gatcatcttc caggacccgt acgcgtcgct
       61 gaacccgcgc atgacggtcg gcgcgatcat cggcgaggcg ctgatcatcc acaagcttgc
      121 caggacgcgg cgcgagttcg aggaccgcat cgtgcagttg ctcgagaccg tggggcttgc
      181 cgccgaccac atgcgccgct acccgcacga gttctccggc ggccagcggc agcgcatcgg
      241 catcgcgcgc gcgcttgccg tatcgccgaa gctcatcgtg tgcgacgagg cggtgtccgc
      301 gctcgacgtg tcgatccagg cgcaggtgat caacctgctg gaggacctgc gcagccagtt
      361 caacctgacc tacatcttca tcgcgcacga cctgtcggtg gtcgagcaca tcagccaacg
      421 cgtggcagtg atgtatctgg gccgcatcgt cgagatcgcg cccgcgcgct cgctgtacac
      481 gaaccccaag catccgtaca ccgaggcgct gctggcggcg gtgccgatcc cggacccgac
      541 ggtcaagcgc aagcgcatcc cgctgcaggg cgacgtgccg agcccactga atccgccgtc
      601 cggctgtcca ttccacacgc gctgcccgat cgcgcagcag ccgctgtgcg ctacgcaaac
      661 gccgccactg aagcagaccg gcgccggcca ctgggtcgcc tgccatctgc gcgggtaagc
      721 ggatgaacac cgcgctgctc gtgatcgatg tgcagcaggg cctgtgcgaa ggcgagtccc
      781 cggcgttcgg gtcgcagcag gtcatcgagc ggatcaaccg cgtcacggac caggcgcgcg
      841 cggccggtgc gagcgtgatc ttcgtgcagc acgagtcggc ctccgaactt gaattcggca
      901 ccgacgcgtg gcggctcgcg cgcggcctgc acgtgcagcc cggcgacctg cgcctgcgca
      961 agacaacgtg cgactcgttc caccgcaccg agttgcagga gaccctgcag cggcacgcgg
     1021 tcacgcggct cgtgatctgc ggcatgcaca cggagttctg cgtcgacacc accgtgcgcc
     1081 gcgcgctggc gctcggctac gaggtcgtgc tggtcgccga cgcgcacacg accgcggacc
     1141 ggccgcatct cgccgcggcg cagatcatcc ggcatcacaa cgagacgctg gcgaacatcg
     1201 agagcttcgg ggtgcgggtg cgcgccatcc cgcacggcga attgactttc ggcgcctgac
     1261 cgatcgccgg agcgggagcg atcggcacgg gaccgaagcc gtcacttcat ctcggtccac
     1321 tgccccgaat aatcgggcgg gtaaccgctg gcggacccgg gcacgacgtt gccattgtgg
     1381 tcgcgccaca cggttgacca ggcgttgtcg accgtcttgc aggtgccgag gtcgtcgcag
     1441 acgcgacggc tgccgcgatt ggcgatgtcg cgccgcgctt ccacgccggg gtcgcgcgtg
     1501 atcttcggca aattggcggt attcggatgg ttccagccgc cggccgagcc ctgcgcagcc
     1561 tgctgcctgg cgtgctggat cgtcgtccgc gtgatggcat tgctcatctg catcatcgag
     1621 ttcgcgtact ggccacccgc ctgcgccacg cgcatttccc attgcggatt catcttctgg
     1681 ctcgccagca tcgccgacag cacgtacttg gcgaagtagc cctgcctggg atcacgggtg
     1741 atgaatcccg agagctggta gacgaaccag ccggagaccc cttgaccgta ggccggacgt
     1801 accaggagcg tcgtggccag cacgaagcct tcgccctcgg ggcaacggaa gatcgcctcg
     1861 ccgaccgagg cgctcgacgc cagacccgtg ccctggccga agcgtgcgat ctcggcgctc
     1921 agggtctggg attcgcgccg caggatcccc gactgcgcaa tccgcggctg gcggcagagc
     1981 ttccaggccg gatagcgctc cgcgaactgg acacccgtca tgtagcgcgc gatcagcccc
     2041 ccggcaacca tttgcccttc gcgccagccg gccatctgcg tggccgcatc cggctcgcgg
     2101 cggggcacga ggtcatagtc gccgatgaag atgcggatcg agccgtccgg cgagaccgcg
     2161 ctcaacgcgg agcgcacgtc gagcggcgag aaccgccgca cgccgccgct gatgcgccaa
     2221 cccttcggca cgtcgatcgt gtaggcgccc tcgttcggct cgcgccaggt ttcgaaggtg
     2281 accgcgccgg tgccgcgctc ggtcgcagcc gaggccacca tcg
//
LOCUS       DBNHBY010000245         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1232866, whole genome shotgun sequence.
ACCESSION   DBNHBY010000245 DBNHBY010000000
VERSION     DBNHBY010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1232866"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="AC16HB_06060"
     CDS             complement(<1..1757)
                     /locus_tag="AC16HB_06060"
                     /inference="COORDINATES: protein motif:HMM:NF053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimL-related protein"
                     /protein_id="HIR5606389.1"
                     /translation="MADLARRLRPRFQVALLGWIRGERAEENLAHLADIATQFERAAS
                     TQPLFQLWWVVGAVLEALQSGGVDANVSVKRLLGHVDREMKRLHEQGEQQYSAAPPVE
                     VLNNLLYYVAQSTVAGERISDVRRSFKLDDMVASRDAEMPDADTMSAPSVRLMRTVAA
                     AIREDLTRVKDVLDIFVRKGATQVDDLAPQLEMLRKISDTLGVLGLGALRARVDSELE
                     SLRAIVERRIAPDDTALLSIAAALIAVEDSLDSQLVRLILPGGTPTDAEPGEPTDEEF
                     RLVQEAVLRECIINMARIKEAVTQSLSAPAEAQGLDQVPQLVRGITAGLLMLGKQRAV
                     EVMEAIGRALGTVVRPEPNALAPARLDRLADAIVAVEYYMETLQTGRADQWQMLDAAE
                     ASLGGIEPVSARVIPLREAAPAAAAASDESVASEVAASVPAPEPAPSAAASAPSTVAK
                     PPVVVTAVDPEFVALFVEEAREHVARIAELFPRWEQNPLDAEALRDLRRAFHTLKGSG
                     RMVGAQRVGEFAWSVESLLNRVISATLARSPDILNVVRDAVATMPQLIDEIDGGAGAT
                     ADVAGIMARADALSGREGPA"
BASE COUNT          251 a          642 c          627 g          269 t
ORIGIN      
        1 gccgggcctt cgcgccccga cagcgcatcg gcgcgcgcca tgatgccggc gacgtcggcg
       61 gtggcgcccg cgccgccgtc gatctcgtcg atcagttgcg gcatcgtcgc caccgcatcg
      121 cgcacgacat tgagaatgtc gggcgagcgc gccaacgttg cgctgatgac gcggttgagc
      181 agggattcga cgctccacgc gaactcgccc acgcgctggg caccgaccat gcggccgctg
      241 cccttcagcg tgtggaacgc gcgcctcagg tcgcgcagcg cttcggcgtc cagcgggttc
      301 tgctcccagc gcgggaacag ttcggcgatg cgcgcgacgt gctcgcgcgc ctcctcgacg
      361 aagagcgcaa cgaattccgg gtcgacggcc gtcacgacca ccggcggctt cgccacggtt
      421 gatggtgcag atgcagcggc gctcggcgcg ggttccggcg caggtacgga agcggcgacc
      481 tcgcttgcga ccgactcatc ggacgccgct gcagcggcgg gtgccgcttc gcgcagcggg
      541 atgacgcggg cggagacggg ttcgatgcca ccgagactcg cctcggccgc gtccagcatc
      601 tgccactggt cggcgcggcc cgtctgcagc gtctccatgt agtactcgac cgcgacgatc
      661 gcatcggcca ggcgatcgag ccgggccgga gccagcgcat tcggttcggg gcgcacgacg
      721 gtgccgagcg cacggccgat cgcctccatg acttcgaccg cccgctgctt gcccagcatc
      781 agcaggccgg cggtgatgcc gcgcacgagc tgcggcacct ggtcgaggcc ctgcgcctcg
      841 gcgggcgcgg acagggactg cgtcaccgcc tccttgatgc gcgccatgtt gatgatgcat
      901 tcgcgcagga ccgcttcctg cacgagacgg aactcctcgt ccgtcggctc gccgggctct
      961 gcgtccgtcg gtgtcccgcc gggcaggatc aggcgcacga gctgcgagtc gaggctgtcc
     1021 tcgaccgcga tcagcgcggc cgcgatgctg agcagcgcgg tgtcgtccgg agcgatgcgg
     1081 cgctcgacga tcgcccgcag cgattcgagc tcgctgtcga cgcgtgcgcg cagcgcgccc
     1141 aggccgagca cgcccagggt gtcgctgatc ttgcgcagca tctcgagctg cggcgccagg
     1201 tcgtccacct gcgtcgcgcc cttgcgcacg aagatgtcca gcacgtcctt gacgcgcgtc
     1261 aggtcctcgc ggatcgcggc cgccacggtg cgcatcaggc gcacgctggg cgccgacatg
     1321 gtgtcggcat ccggcatctc ggcgtcgcgg ctcgcaacca tgtcgtcgag cttgaacgag
     1381 cggcgcacgt cgctgatgcg ctcgcctgcc accgtactct gcgcgacgta atagagcagg
     1441 ttgttcagga cctcgaccgg cggcgcggcg gagtactgct gctcgccctg ttcgtgcagg
     1501 cgcttcatct cgcggtcgac gtggccgagc aggcgcttga ccgacacgtt cgcgtcgaca
     1561 ccaccggact gcagcgcctc gagcactgcg ccgaccaccc accacagctg gaacagcggc
     1621 tgcgtcgagg ctgcgcgctc gaactgcgtg gcgatgtccg cgagatgggc caggttctcc
     1681 tccgcacgtt caccgcggat ccagccgagc agcgcgacct ggaagcgcgg ccgcaggcgg
     1741 cgcgcgagat cggccacctc tcggtcgccg gcgaagggcg atgacggac
//
LOCUS       DBNHBY010000246         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_759354, whole genome shotgun sequence.
ACCESSION   DBNHBY010000246 DBNHBY010000000
VERSION     DBNHBY010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_759354"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="AC16HB_06065"
     CDS             <1..795
                     /locus_tag="AC16HB_06065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5606390.1"
                     /translation="TLRKVSDDLARQQQEKVQFTSRLMTMGEMASSLAHELNQPLTAI
                     TNYSEGTLARLKERQISIDELQKALEKTSVQAQRAGGIIRRIREFVKRSEPRRRPTPP
                     HRIVEDAIGFAEIEANKKRIQIAASVDPTLEPLLVDPLLIEQVLLNLLKNAIDAMEHA
                     TVRRIDVVVRRSLLDGMAEFAVIDRGSGIPEENLAMLFEPFFSTKTEGMGMGLNICRS
                     IIEFHQGRLTVEKNPERAGGTIMRFTLPITGEVGADAPAVQPTASQ"
     gene            792..1415
                     /locus_tag="AC16HB_06070"
     CDS             792..1415
                     /locus_tag="AC16HB_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5606391.1"
                     /translation="MSTNESAARGVAYVIDDDEAVRDSLQFLLEPDYRVESYESAEAF
                     LARYDPQAIACLILDVRMPGMSGLQLQELLLAREANLPITFITGHGDVPMAVSTMKKG
                     AADFIEKPFDRSELKALVEKMLAHARARATEIERKRINAALLARLTPREQQVLERIVA
                     GRLNKQIADDLGISIKTVEAHRANIMDKLKAGTVADLMRVALASGSG"
     gene            complement(1451..>1659)
                     /locus_tag="AC16HB_06075"
     CDS             complement(1451..>1659)
                     /locus_tag="AC16HB_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606392.1"
                     /translation="PDDHQIAMRWRSLDEHFVAVGPQFLAEMLGNEIAETNDRVIVAL
                     LGGTAEACGRAARQSQVAGWMTRL"
BASE COUNT          338 a          541 c          534 g          246 t
ORIGIN      
        1 acgctgcgca aggtcagcga cgatcttgcg cgccagcagc aggagaaagt gcaattcacc
       61 tcgcggctga tgacgatggg cgaaatggct tcgtcgctcg cgcacgagct gaaccagccc
      121 ttgaccgcca tcaccaacta cagcgagggc acactggcgc ggctgaagga gcggcagatc
      181 tcgatcgacg aactgcagaa agcgctggag aagacctccg tgcaggcgca gcgcgccggc
      241 ggcatcattc gccgcattcg cgaattcgtg aagcgctccg agccgcgccg ccggccaacg
      301 ccgccgcacc ggatcgtgga ggatgccatc ggcttcgccg aaatcgaggc aaacaagaaa
      361 cgcatccaga tcgccgcaag cgtcgatccg acacttgagc cgctgctggt cgatccgctg
      421 ctgatcgagc aggtgctgct caatctgctg aaaaacgcga tcgacgcgat ggagcacgcg
      481 accgtgcgcc gcatcgacgt cgtcgtgcgc cgctcgctgc ttgacggcat ggccgagttc
      541 gcggtgattg accgcggcag cggcattcca gaggaaaacc ttgccatgct gttcgagccg
      601 ttcttcagca ccaagaccga aggcatgggc atgggcctga acatctgccg ctcgatcatc
      661 gaattccacc agggccggct gacggtcgag aagaatccgg agcgcgccgg cggaacgatc
      721 atgcgcttca cgctgccaat caccggcgag gtcggcgccg atgcgccggc ggtgcagccg
      781 accgcaagcc aatgagcacg aacgaatcgg ccgcccgggg cgtcgcctac gtcatcgacg
      841 acgacgaagc ggtgcgcgac tcgctgcagt tcctgctgga gcccgactat cgcgtcgaaa
      901 gctacgaaag cgcggaagcc tttctggcgc gctatgaccc gcaggcgatc gcgtgcctga
      961 tcctcgacgt gcgcatgccg ggcatgagcg ggctgcagtt gcaggaactg ctgctcgcgc
     1021 gcgaggccaa cctgccgatc accttcatca ccggccacgg cgacgtgccg atggcggtgt
     1081 cgacgatgaa aaaaggcgcg gccgacttca tcgaaaaacc gttcgatcgc agcgaactga
     1141 aggcgctggt cgagaagatg cttgcgcacg cacgcgcgag ggcgaccgag atcgagcgca
     1201 aacgaatcaa cgccgcgctg ctggcccggc tgacgccgcg cgagcagcag gtgctcgagc
     1261 ggatcgtcgc cggacgcctg aacaagcaga tcgccgacga cctcggcatt tcgatcaaga
     1321 cggtcgaggc gcaccgcgcc aacatcatgg acaagctgaa ggcaggcacg gtggcggacc
     1381 tgatgcgggt ggcgctggcg agcggctcgg gatgatgcgg ccggccgcca gcaccttgcc
     1441 ccgcatgaaa ctacagtcga gtcatccagc ccgccacttg cgactgcctc gccgcgcgac
     1501 cgcacgcttc tgccgtgccg cccagtaaag cgacgatgac gcggtcattc gtctccgcga
     1561 tctcgttgcc gagcatctcg gcaaggaatt gcggtccaac tgccacgaaa tgctcgtcga
     1621 gtgaacgcca gcgcatcgcg atctggtggt catcgggca
//
LOCUS       DBNHBY010000247         2836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_488453, whole genome shotgun sequence.
ACCESSION   DBNHBY010000247 DBNHBY010000000
VERSION     DBNHBY010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_488453"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="AC16HB_06080"
     CDS             <1..1470
                     /locus_tag="AC16HB_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606393.1"
                     /translation="FMVDLFKVSDPSEALGNTITAREILDKGAARIDRELARQPAIQA
                     TLMDTMGTVYTSLGLYDAAIPLVRKAYERRLQLWGPEHAEVASSLNHLGEVLTLKSDY
                     AGAERSLRDALSVQRRVHGPQSDAAAQSLTLLADVLRRRGDLAGAESAINEALAIRRK
                     LHGNAPNGDVAVGLETLGIIYTEAGRYEDAVKVMREVAAMQRKLHPAAHPDVARAMDN
                     LSNALMNVGRPDEAEPLARLAVSTYRLVYGEDHPATATGLNNLAYVLEARERYAEAES
                     TYRSALEINRRLLGPRHPTISLMMSNIAYVQYARGDESGAIATMRESLAIAREALGPE
                     HAEVASRAAALGQWLLDAGSMSEAREMIRVALDVRRKALGEQHPAYLNTLALEANLAL
                     AEGDPTRAQALGRDARIGLEKALPAGSWQIANAMSVEGAALVQLGRYAEAEPLLRDSQ
                     AGLAKAPVPRLAERGRERLAELYRKSGRPEDARKLREQG"
     gene            1506..2660
                     /locus_tag="AC16HB_06085"
     CDS             1506..2660
                     /locus_tag="AC16HB_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR5606394.1"
                     /translation="MFLMATSAIGPGFITQTTQFTAQLGAAFAFAIVVSVLIDIALQL
                     NVWRVIGVSGLRAQELAQRVRPGAGHVLAALVVLGGFVFNVGNVAGAGLGLEAMLGLD
                     PRYGGLLSALLAVFIFLSRRAGAAMDRSVVALGFLMIAMTGYVAFVSQPPYGAALRNA
                     VLPDHVDVLAITTLVGGTVGGYITYAGAHRMLDAGIVGVAHVGEISRASVSGVLLTAL
                     MRVLLFLAVLGVVAAGTALDAANPAASAFEAAAGEVGRRLFGLILWAAAITSVIGASY
                     TSVSFLTAPGPAGEPRRRKWVLGFIAASTAVFVFAGAAPVPLLIFAGAFNGLILPLGV
                     GILLWVAWRRADLLGGYRYPTALVACGLAAWALTVYLGWQSVARLATLFQ"
BASE COUNT          405 a          962 c         1010 g          459 t
ORIGIN      
        1 ttcatggtgg acctgttcaa ggtgtccgac ccgagtgaag cgctgggcaa caccatcacc
       61 gcacgcgaga tcctcgacaa gggcgctgcg cgcatcgacc gcgagctcgc ccgccagccg
      121 gccatccagg cgacgctcat ggacaccatg ggcacggtgt acacgagcct cggcctgtac
      181 gacgcagcga taccactcgt gcgcaaggct tatgagcgcc gcctgcagct ctggggtccg
      241 gagcacgccg aggtcgcgag cagcctgaat cacctcggcg aagtgctcac gctgaaatcc
      301 gattacgccg gagccgaacg cagcctccgt gatgccctgt cggtgcagag gcgcgtgcac
      361 ggcccgcaga gcgatgccgc ggcgcagtcc ctgacgctgc tggcggacgt gctgcgaagg
      421 agaggcgatc tcgcgggcgc cgagtccgcg atcaacgagg cactggccat tcgccgcaag
      481 ctgcacggga acgcccccaa tggcgacgtc gcggtcgggc tggaaaccct cggcatcatc
      541 tacaccgagg cgggacgcta cgaggacgcg gtcaaggtga tgcgcgaggt cgccgccatg
      601 cagcgcaagc tgcatcccgc agcacaccca gacgttgccc gtgcaatgga caacctgtcc
      661 aacgccttga tgaacgtcgg tcgacccgac gaagccgagc cgctcgcgcg cctggccgtg
      721 tcgacctacc ggctggtcta tggcgaggac catccggcca ctgcgacggg cctgaacaac
      781 ctcgcctacg tgctggaggc gcgcgagcgc tacgccgagg cggagtcgac ctatcggtcc
      841 gcgctcgaga tcaaccgccg cctgctcggg ccgcggcatc cgaccatttc gctgatgatg
      901 agcaacatcg cctacgtgca gtacgcacgc ggtgacgaat ccggtgcgat cgccacgatg
      961 cgtgaatcgc ttgcgatcgc gcgcgaggcg ctgggaccgg agcacgcgga agtggcgagt
     1021 cgcgccgccg cgctggggca gtggctgctc gatgcgggca gcatgtccga ggcgcgcgag
     1081 atgatccgcg ttgcgctcga cgtccgccgc aaggcactcg gcgagcagca tcctgcatac
     1141 ctgaacacgc tggccctgga agcgaacctg gcgctggcgg agggcgatcc gacgcgggcg
     1201 caggcgctgg gtcgcgatgc gcgcatcggc ctcgagaagg cgctgcccgc cgggagctgg
     1261 cagatcgcca acgccatgag cgtcgaaggg gcggcgctgg tgcagctcgg gcgttatgcg
     1321 gaagctgaac ctctgctccg cgacagccag gcgggcctgg cgaaggcgcc ggtgccgcgc
     1381 ctcgcggagc gcggtcgcga gcgcctcgcc gagctctacc ggaaatcggg gcgccccgag
     1441 gacgcacgca agctgcgaga gcaggggtga cagatacgcg atcccggagc gggctcgccg
     1501 gcgccatgtt cctgatggcc acctcggcga tcggtccggg cttcattacg cagaccacgc
     1561 agttcaccgc gcagctcggc gccgcttttg cttttgcgat cgtggtatcg gtgctgatcg
     1621 acatcgcgct acagctcaac gtctggcgcg tcatcggcgt gtcggggctg cgcgcgcagg
     1681 agctggcgca gcgcgtgcgg ccgggcgccg gccacgtgct tgcggcgctc gtggttctcg
     1741 gcggattcgt gttcaacgtc ggcaacgtcg cgggcgcggg cctgggactc gaggcgatgc
     1801 tgggcctcga cccgcgttac ggcggcctgc tgtccgcgct gctcgcggtg ttcatcttcc
     1861 tgtcgcgccg ggccggcgct gcgatggatc gcagcgtcgt ggcgctcggc ttcctgatga
     1921 tcgccatgac gggctacgtc gcgttcgtgt cgcagccacc ctatggcgcg gcgctgcgca
     1981 acgcggtgct gcccgaccat gtcgacgtgc tcgcgatcac gacgctggtc ggaggtacgg
     2041 ttggcggtta catcacttac gccggcgcgc atcgcatgct cgatgcgggc atcgtcggcg
     2101 tcgcccacgt aggcgagatt tcgcgtgcgt cggtcagcgg cgtgctgctg acggccttga
     2161 tgcgcgtgct gctgttcctc gcggtgctcg gcgtcgttgc agcgggcact gcgctcgacg
     2221 cggcgaaccc ggcagcatcg gcgttcgagg cggcggcggg cgaggtgggg cgccggctgt
     2281 tcggcctcat cctgtgggcg gcggccatca cctcggtgat cggcgcttcg tacacctccg
     2341 tcagcttcct caccgcaccc ggacccgcgg gtgagccgcg tcgccgcaag tgggtcctgg
     2401 ggttcattgc ggcgtcgacg gcggtcttcg tcttcgcggg cgctgcgccg gtgccgctgc
     2461 tgatcttcgc tggcgctttc aacggcctca tcctgccgct tggcgtcggg atcctgctgt
     2521 gggtggcctg gcgccgtgcc gacctgctgg gaggttaccg atacccgacg gcgctggtcg
     2581 cctgcggcct cgctgcctgg gcactgacgg tgtacctcgg ctggcagtcc gtggcgcgcc
     2641 tcgccacgct gttccagtag ccgctggcag cgcgcgtccc gcgcttccgt ctggcagcca
     2701 gacgctgccg cttcgtcgta cgattcggtc atgcagaaga agacgcacga cgaagccggc
     2761 gccgcgcgcg cgaaccggct cgctgaattc ctgctggaaa acgggctgtc aggggccggg
     2821 ccgctgtcga gcgcgc
//
LOCUS       DBNHBY010000248         8382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_691259, whole genome shotgun sequence.
ACCESSION   DBNHBY010000248 DBNHBY010000000
VERSION     DBNHBY010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_691259"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..240)
                     /locus_tag="AC16HB_06090"
     CDS             complement(10..240)
                     /locus_tag="AC16HB_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606395.1"
                     /translation="MVRTLKPYIAVRPERLLAAALLAVAAAAGALAARLARSEQRVCT
                     REFGTIELDGRTMGAVYEDGRLVGVLPGVGRL"
     gene            complement(285..446)
                     /locus_tag="AC16HB_06095"
     CDS             complement(285..446)
                     /locus_tag="AC16HB_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606396.1"
                     /translation="MKVRRIRLPSPRNPLALIVRFRRAGAHGTSGKAMRRRAATALRR
                     EIQALKEGP"
     gene            complement(772..1323)
                     /locus_tag="AC16HB_06100"
     CDS             complement(772..1323)
                     /locus_tag="AC16HB_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606397.1"
                     /translation="MSVRDLARVRLDNALLLFDEFVRQAARTPDAATLRGLEGRFADR
                     LQIQPSYWSQIKSRARQIGERLARQFEQRMHKPYGWMDVPHDAADAARKPVPAAATAP
                     APEPESPLPVDDDERFIVGLVLTYYRRHPQRARTRLLDLLGEVLMPGAAGAAAPPPRA
                     PAVSDAEAWQRSQRGVAPLKRKR"
     gene            1580..2812
                     /locus_tag="AC16HB_06105"
     CDS             1580..2812
                     /locus_tag="AC16HB_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606398.1"
                     /translation="MSPDSALVDSPAAWRRLVAAVLLSTLGGVGMWSFVVALPPVAAE
                     FGLSRSAASTPFSALMIGFAAGGVLMGRWADRAGVMTPVLAGTVALAAGYVAAGLATD
                     PLQFTLAHGVLIGFAGASATFGPLLADTSHWFLRRRGLAVAIAASGNYFAGTLWPPVV
                     QALIDQVGWRHAHIAIGVFCLVTMLPLALALRRRAPLHDDANGAGTRAVAARPLGLHP
                     NALQLLLIIAGVACCVAMSMPQVHIVAYCADLGYGAARGAEMLSLMLGFGIVSRLASG
                     WIADRIGGLATLLLGSALQGLALLLYVPFDGLTSLYVISALFGLFQGGIVPSYAITVR
                     EYFAPQEAGARVGTVLMATLAGMALGGWLSGAVFDLTGSYRAAFINGIGWNLLNLSIA
                     GWLLLRARSRPRAGTAIA"
     gene            2809..3864
                     /locus_tag="AC16HB_06110"
     CDS             2809..3864
                     /locus_tag="AC16HB_06110"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR5606399.1"
                     /translation="MRAFDLPPPQTGPAAWRGPEMAERDDWIVQLAPGAIAELEAAMR
                     GVIARGLDIARLAPADFPLPTLAARIDGLRRELLHGRGFALLRGLPIERYSRLEAATI
                     FLGLGSHLGRARSQNASGHVLGHVCDLGLSSADPNVRIYQTHERQTFHTDSCDVVGLL
                     CLREAKSGGDSLLVSALAIFNELRAARPDLLARLLAPMAHDRRGEVPAGALPYFMIPV
                     FSWHADRLTVFYQRQYFDSAQRFVDAPRLTREDIDALDALDALADDPRLNLTMRLVPG
                     DVQFVHNHTLLHDRTAFEDWPDPDRRRHLLRLWLACPGARELPPAFAARYGALTIGDR
                     GGIVVPGTRLHAPLEPQ"
     gene            3984..4418
                     /locus_tag="AC16HB_06115"
     CDS             3984..4418
                     /locus_tag="AC16HB_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5606400.1"
                     /translation="MRLALPLVAALLLAACATPRPDRADAERQVADTERAFARTMAER
                     DHAAFMAFLSDEAVFFSGETPLRGKQAVADAWRRFFAKPAAPFSWRPQTVEALDSGTL
                     ALSTGPVFDPTGKCIGSFLSIWRLEPSGWRIVFDKGGPCPPQ"
     gene            complement(4420..4887)
                     /locus_tag="AC16HB_06120"
     CDS             complement(4420..4887)
                     /locus_tag="AC16HB_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5606401.1"
                     /translation="MSANTIDELPLFKQFRPRNIDKLRALARPMCFEPGQRVFREGDA
                     SSLFYLIKSGHVALELEAPGRAMRVQIQGPGDEFGWSALLPGRGMQFQARTMDAVEAF
                     AIEGAELLALCRDDPAFGFELMHHLLGLVADRLAHTRVQLSDFYSPQAKRAGA"
     gene            complement(4956..6407)
                     /locus_tag="AC16HB_06125"
     CDS             complement(4956..6407)
                     /locus_tag="AC16HB_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5606402.1"
                     /translation="MKLAEYARYDALGLAELVARREVTPRELAATAARAIAAANPAVN
                     AVVETYPDRIEGLDETTLGAGPFRGVPFLIKDVFGHEAGRLIEFGSRLCRGMRVQQST
                     HYLELLQASGVNVLGRSAAPEYSMSGTTESALYGNTSTPWRHGYSAGGSTGGGMAAVV
                     AGMVPVAHGSDIGGSIRIPASFCGGVGLKPSRGRVSFGPMLDENGYGLAQTFVQTKTV
                     RDAAAMLDCLAIPQPGDPFVIPKPAETYATLIGARAPRLRIAWSTDALMGVPTDPEVA
                     AAVERTARMLADMGHAVEHVNVQFDDLEALRRMMDVWFFGFDLRLALYSRRSGHAIGP
                     DTLEPVIYRIYEHARRMTAAQFLDAMTALNGARRSLGRIFVACDVWLTPTTARVAEPW
                     GNYNIGRADVAYEEIPEKLLRVPCQFTLPHNIMGTPAVSLPLAMHSSGLPIGVQLGAR
                     PAQEHLLLQLATALEAAAPWAQRVPPLHVSHLD"
     gene            6466..6771
                     /locus_tag="AC16HB_06130"
     CDS             6466..6771
                     /locus_tag="AC16HB_06130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5606403.1"
                     /translation="MILEVADIRIHPGKQAEFDTAIRRGVESVIAKAKGFRGFKVNKG
                     IESPERYLLMIFWDTLENHTVDFRGSDAFTAWRAIVGPFFAQPPQVEHFTLLAKSGA"
     gene            6768..7436
                     /locus_tag="AC16HB_06135"
     CDS             6768..7436
                     /locus_tag="AC16HB_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5606404.1"
                     /translation="MTKAALIVGAGSGISASFARLLAREGHAVALASRGGAARSGEVP
                     AALKLACDATNPDDVERLFARLDTELPPLDVVLYNPSYRARGPITELAPDDVRRSLEV
                     TAFGAFLVAQQAARRMLARGSGALLFTGASASVKGYAQSAPFAMGKFALRGLAQSLAR
                     ELAPQGVHVAHFVIDGAVRAGGSTDDRQLDPDAIAAAYLAIVNQPRSAWTWEVELRPW
                     VEKF"
     gene            complement(7438..>8382)
                     /locus_tag="AC16HB_06140"
     CDS             complement(7438..>8382)
                     /locus_tag="AC16HB_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR5606405.1"
                     /translation="YSMTQQAIHLGFLPRMAITYTSAKEMGQIYMPAVNWMLLAAVLV
                     VTTGFRSSSNLAGAYGIAVTLMMLVTTFLTFFVVRHGWRYPLWLALAATAFFILLDAV
                     LVTACSAKFFDGGWFTLALGLVLFAVMATWKRGRELLFEHIHRADPEVKGFIDLLLRD
                     DVRRVAGTAVYAVGDPERVPQALLHNLKHNKVLHERVVILNVVFREVPWISFDKRVEV
                     EPLARGVWKVRVDYGFMNQPDIPQALTLCRPYGLDINLFETSYFLSRETVVPTARGIM
                     ARWRERLFETLSRNAASVVELFRIPPNCVIELGTRVQL"
BASE COUNT         1211 a         2943 c         2916 g         1312 t
ORIGIN      
        1 cgaccgcgct cacagccgtc ccacgccggg cagcacgccg accaggcgac cgtcctcgta
       61 caccgcaccc atcgtgcgac cgtcgagttc gatcgtgccg aattcgcgcg tgcacacgcg
      121 ttgctcactt cgcgcgaggc gtgcggcaag tgcccctgct gcggctgcca cggcgagcag
      181 tgccgcggca agcagacgct ccggccggac ggcgatgtaa ggcttcaatg tgcggaccat
      241 cttcttctcc ttgggtgtcg ggcgaccggc agagttcgtt cagatcacgg gccttccttc
      301 agcgcctgga tctcgcggcg cagtgccgtc gcggcgcgcc gtcgcatggc tttgccggag
      361 gtgccatgcg ccccggcgcg gcggaagcgc acgatgagcg cgagcggatt gcgcggggac
      421 ggcaagcgaa tgcgcctgac tttcatctcg atctccttgc ggtgtgcaga tccgaccgcc
      481 cgcgcggttt gccggcgtat cggttgggct gaaaacagtc cggcccgggg cagcagcgct
      541 gcaacccggg ccggggagcg gcgcgacgcg acgttcagcg cgcggcgacc ccggcggcag
      601 tcacagcggc gacggcgcag ccaaacgggg aaccgacggc ggcgcagccg atttgcagtg
      661 gcgtcatgcg gctcctttcc cgttgcgcgt tgaaccgatg tgcagcattg gcgcgcatga
      721 taaataaaaa tttatcttgc gcaagaacta ttttgcggga gggcaagatc ttcagcgctt
      781 gcgtttcagc ggggccacgc cgcgctggct gcgctgccac gcttccgcgt cgctgaccgc
      841 gggcgccctc ggcggcggtg cggcggcgcc ggcggcgccc ggcatcagca cttcgcccag
      901 cagatcgagc agccgcgtgc gcgcgcgttg cggatggcgt cggtagtagg tcaacacgag
      961 cccgacgatg aagcgctcgt cgtcatcgac gggcaacggg ctctcgggct cgggcgccgg
     1021 ggcggtcgcc gccgccggca caggcttgcg cgccgcgtcc gctgcatcgt gcggcacgtc
     1081 catccacccg tacggcttgt gcatccgctg ctcgaactgg cgcgccagcc gctcgccgat
     1141 ctgccgcgcg cggctcttga tctggctcca gtagctcggc tgaatctgca gacggtccgc
     1201 gaatcgccct tcgagcccgc gcagcgtggc ggcgtcgggc gtgcgcgccg cctgccgcac
     1261 gaactcgtcg aacagcagca gcgcgttgtc cagacgtacg cgcgccaggt cgcgcacgct
     1321 catctcagtc gtcgccccgg tttcatttgc ccagatgata aaggccgcac acgccggcgc
     1381 cgcggatcgc gtcgacaaat ttgaagtgcg ctggtttcgc accagcacgc tccgggcctt
     1441 cgtgcccggc gcggcatcgt tgcaccgaag gtgatcgcgc cgattgcgct cgaaggccac
     1501 gaccgcgcag ccggccgcgt acgcgtgagt gggcggggcc acgcgccggg taagctcgcg
     1561 gtctgcccgc gagcctgcct tgagccctga ttccgcactc gtcgactcgc ccgcggcgtg
     1621 gcgccgcctc gtcgcggcgg tattgctgtc gaccctcggc ggcgtcggca tgtggtcgtt
     1681 tgtagtcgcc ctgccgccgg tggcggcgga gttcggactg tcgcgctcgg ccgcatcgac
     1741 gccgttttcg gcgctgatga tcgggtttgc cgccggcggc gtgctgatgg gccgctgggc
     1801 cgatcgcgcc ggcgtgatga cacccgtgct ggcgggcacc gtcgcactgg ccgcgggcta
     1861 tgtcgccgcg gggctggcga cggacccgct tcagttcacg ctcgcgcacg gcgtgctcat
     1921 cggattcgcc ggcgcgtcgg ccacgttcgg gccgctgctc gccgatactt cgcactggtt
     1981 tctgcgccgg cgcgggctcg cggtggcgat cgccgcgagc ggcaactact tcgccggcac
     2041 gttgtggccg ccggtggtgc aggcgctgat cgatcaggtc ggctggcgcc acgcgcacat
     2101 tgcgatcggc gtcttctgcc tggtcacgat gctgccgctc gccctggcgc tgcgccggcg
     2161 tgcgccgctg cacgacgacg ctaacggcgc cggcacgcgc gcggtggcgg cgcgcccgct
     2221 cggactgcac ccgaacgcgc tgcaactgct gctaatcatc gccggcgtgg cgtgctgtgt
     2281 cgcgatgtcg atgccgcagg tccacatcgt cgcgtactgc gccgacctcg gttacggcgc
     2341 ggcacgcggc gccgagatgc tgtcgctgat gctcggtttc ggcatcgtca gcaggctggc
     2401 gtcggggtgg atcgcggatc gcatcggcgg actggccacg ctgctgctcg gatctgcgct
     2461 gcaggggctg gcgctgctgc tctacgtacc gttcgacggt ctgacctcgc tgtacgtgat
     2521 ctcggcgctg ttcggtctgt tccagggcgg catcgtgccg tcgtacgcga tcacggtgcg
     2581 cgagtacttc gctccgcagg aggccggcgc gcgcgtcggc accgtgctga tggcgacgct
     2641 cgccggcatg gcgctgggcg gctggctgtc gggcgcggtg ttcgacctga cgggatccta
     2701 tcgggccgcg ttcatcaacg gcatcggctg gaacctgctg aacctgtcga tcgccggctg
     2761 gctgctcttg cgcgcccgca gccgaccgcg cgcgggcacc gcgatcgcat gagggcgttc
     2821 gacctgccgc cgccgcagac cgggcccgcc gcgtggcgcg ggcccgagat ggcggagcgc
     2881 gacgactgga tcgtgcaact cgcgcccggc gcgattgcgg agctcgaagc ggcgatgcgc
     2941 ggcgtgatcg cgcgcggcct cgacatcgcg cgactggcgc ccgccgactt cccgctgccg
     3001 acgcttgcgg cgcggatcga tggactgcgg cgcgagctgc tgcacggccg cggcttcgca
     3061 ttgctgcgcg gtctgcccat cgagcgctat tcgcgcctcg aagcggcgac gatctttctc
     3121 ggactcggca gccatctcgg ccgtgcgcgc tcgcagaacg ccagcgggca cgtgctcgga
     3181 cacgtgtgcg acctcgggct gtcgagcgcc gatccgaacg tgcgcatcta ccagacgcac
     3241 gagcggcaga cctttcacac cgactcgtgc gacgtggtcg gcctgctgtg cctgcgcgaa
     3301 gcgaagtcgg gcggcgactc gctgctggtg agcgcgctcg cgatcttcaa cgaactgcgc
     3361 gcggcccgcc ccgatctgct ggcgcggctg ctggcaccga tggcgcacga tcgccgcggc
     3421 gaggtgccag ccggcgcgct gccgtacttc atgatcccgg tcttcagttg gcatgcggat
     3481 cggctcactg tgttctacca gcggcagtac ttcgacagcg cgcaacggtt cgtcgacgcg
     3541 ccgcggctga cgcgcgagga catcgacgcg ctcgacgcgc tcgacgcgct cgccgacgat
     3601 ccgcggctga atctgacgat gcgcctggtg cccggcgacg tgcagttcgt gcacaaccat
     3661 acgctgctgc acgatcgcac cgcgttcgag gactggcccg acccggatcg ccgccgccat
     3721 ctgctgcgac tgtggctcgc ctgccccggc gcacgcgaac tgccgcccgc gttcgccgca
     3781 cgctacggcg cgctcaccat cggcgaccgc ggcggcatcg tcgtacccgg cacgcggctg
     3841 cacgcgccgc tcgagccgca gtgagcgcga cgcgggcggt gcgcgcacgc agcgtggctg
     3901 caggcggtag gttccggcga atcgagttgc agttgttgcg gatcggcgcg gcagcgaagg
     3961 ctccttcacg tatcgtcgcg gccatgcgcc tcgccttgcc cctcgtcgcc gcgctgctgc
     4021 tcgccgcctg cgcgacgccg cgcccggacc gcgccgacgc cgagcgccag gtcgcggaca
     4081 ccgagcgcgc gttcgcgcgc acgatggccg agcgcgatca cgcggcgttc atggcgttcc
     4141 tgtcggacga ggccgtgttc ttcagcggcg agacgccgct acgcggcaag caggcggtgg
     4201 ccgacgcatg gcgacgtttc ttcgcaaagc cggcggcgcc gttttcgtgg cggccgcaga
     4261 cggtcgaggc gctcgactcg ggcacgctgg cgctgtcgac cgggccggtg ttcgacccga
     4321 ccggcaagtg catcggctcg ttcctgtcga tctggcgcct cgaaccgagc ggctggcgca
     4381 tcgtgttcga caagggcggg ccctgcccgc cgcagtgatt caggcgccgg cgcgcttggc
     4441 ctgcggcgaa tagaagtccg acagctgcac gcgcgtgtgc gcgagccggt cggcgacgag
     4501 gccgagcagg tgatgcatca gctcgaatcc gaatgcggga tcgtcgcggc acagcgcaag
     4561 gagctcggcg ccttcgatcg cgaaggcctc gaccgcatcc atcgtgcgcg cctggaactg
     4621 catgccgcgc ccgggcagca gcgccgacca gccgaactcg tcgccgggcc cctggatctg
     4681 cacgcgcatc gcacgccctg gcgcctcgag ctcgagcgcg acgtgccccg acttgatcag
     4741 gtagaagagc gaactggcgt cgccctcgcg aaacacccgc tggccgggtt cgaagcacat
     4801 ggggcgggcg agcgcacgca gcttgtcgat gttcctgggc cggaactgct tgaacagtgg
     4861 caattcgtcg atcgtgttcg cactcatgcg gacctccgtc gaatgcaaac cactttagcc
     4921 caaccgctcg ccggtgcaag cgcgcatccg cgcagtcagt cgaggtgcga cacgtgcagc
     4981 ggcggcaccc gctgcgccca cggcgctgcg gcctccagcg cggtggccag ctgcagcagc
     5041 aggtgctcct gcgccggccg cgcccccagc tgcacgccaa tcggcagtcc ggacgaatgc
     5101 attgccagcg gcagcgacac ggccggcgtg cccatgatgt tgtgcggcag cgtgaactgg
     5161 cacggcacgc gcagcagttt ctccgggatc tcctcatacg cgacgtcggc gcggccgatg
     5221 ttgtagttgc cccacggctc cgccacgcgc gcggtcgtcg gcgtcagcca cacgtcgcac
     5281 gcgacgaaga tgcggccgag cgaacggcgt gcgccgttca gcgccgtcat cgcatcgagg
     5341 aactgcgccg cagtcatgcg gcgcgcgtgc tcgtagatcc tgtagatgac cggctccagc
     5401 gtgtcggggc cgatcgcgtg cccgctacgg cgcgagtaga gcgcgagccg caggtcgaac
     5461 ccgaagaacc agacgtccat catccgccgc agcgcttcca ggtcgtcgaa ttgcacgttc
     5521 acgtgctcga ccgcgtggcc catgtcggcc agcatgcgcg cggtgcgctc gaccgcggcg
     5581 gcgacctcgg ggtcggtcgg cacacccatc agcgcatcgg tcgaccacgc gatccgcagg
     5641 cgcggcgcac gcgcgccgat cagcgtggcg taggtctcgg ccggcttcgg gatcacgaac
     5701 ggatcgccgg gctgcggaat cgccaggcaa tcgagcatcg ccgccgcatc gcgcacggtc
     5761 tttgtctgca cgaaggtctg cgcgagcccg tagccgttct cgtcgagcat cggcccgaac
     5821 gagacgcgcc cgcgcgacgg cttcaggccg acgccgccgc agaagctggc ggggatgcgg
     5881 atcgacccgc cgatgtccga gccatgcgcg accggcacca tgcccgcgac gaccgccgcc
     5941 atgccgccgc cggtcgagcc gcccgccgag tagccgtgcc gccagggcgt cgacgtgttg
     6001 ccgtacaacg cgctctccgt cgtgcccgac atcgagtact ccggcgcagc agaccgaccg
     6061 aggacattga cgcccgacgc ctgcagcagt tcgaggtaat gcgtgctctg ctgcacccgc
     6121 atgccgcggc acaaccggct gccgaactcg atcagacgtc cggcctcgtg gccgaacaca
     6181 tccttgatca ggaacggcac gccacggaag gggccggcgc cgagcgtggt ctcgtcgagt
     6241 ccctcgatgc gatcgggata ggtctcgacc accgcgttga cggctggatt ggcagcggcg
     6301 atggcgcgtg ccgcggtcgc ggccagctcg cgcggtgtca cctcgcggcg cgcgaccagt
     6361 tcggcgagtc ccagcgcgtc atagcgcgcg tattcggcga gtttcatcgt gcctccgtcc
     6421 tcacgtcgtc agcgctacca tcatgcgcca caccgaggag ccgccatgat cctggaagtc
     6481 gccgatatcc gcatccaccc gggcaagcag gccgaattcg acaccgcgat ccgccgcggc
     6541 gtcgagtccg tgatcgcgaa agcgaagggc tttcgcggct tcaaggtgaa taagggcatc
     6601 gagtcgcccg agcgctacct gctgatgatc ttctgggaca cgctcgagaa tcacacggtc
     6661 gacttccgcg gctcggatgc gttcaccgcg tggcgcgcca tcgtcggtcc gttcttcgcg
     6721 cagccgccgc aggtcgagca cttcacgctg ctggcgaagt cgggcgcatg acgaaggcgg
     6781 ctctgatcgt cggcgctggc agcggtatca gcgcgtcgtt cgcacgcctg ctcgcgcgcg
     6841 agggccatgc ggtcgcgctg gcgtcgcgcg gcggcgccgc gcgcagcggc gaggtgccgg
     6901 ccgcgctgaa gctcgcttgc gacgcgacga acccggacga cgtcgagcgg ctcttcgctc
     6961 ggctcgacac ggaactgccg ccgctcgacg tcgttctgta caacccgagc taccgcgcgc
     7021 gcgggccgat cacggaactg gcgcccgacg acgtgcggcg cagcctcgag gtcacggcgt
     7081 tcggcgcgtt cctcgtcgcg cagcaggcgg cgcgccgcat gctcgcacgc ggaagcggcg
     7141 cgttgctgtt caccggcgcg tcggcgagcg tgaagggcta tgcgcagtcc gccccgttcg
     7201 cgatgggcaa gttcgcgctg cgcgggctgg cgcagagtct cgcgcgcgaa ctggcgccgc
     7261 agggcgtcca cgtcgcgcat ttcgtgatcg acggcgcggt gcgcgccggc ggaagcacgg
     7321 acgaccgcca gctcgatccc gacgcgatcg ccgcggcgta tctcgcgatc gtcaaccagc
     7381 cgcgcagcgc gtggacctgg gaagtcgagc tgcggccgtg ggtggaaaag ttctagctca
     7441 gagttgaacg cgggtcccga gttcgatcac gcagttcggc ggaatccgga acagttcgac
     7501 gacagacgcg gcgttgcgtg acagcgtctc gaacagccgc tcgcgccagc gcgccatgat
     7561 gccccgcgcg gtcggcacca ccgtttcgcg gctgaggaag tacgaggtct cgaacaggtt
     7621 aatgtcgagt ccgtatggac ggcacagcgt cagcgcctgc gggatgtccg gctggttcat
     7681 gaagccgtaa tcgacgcgca ccttccacac gccgcgcgcc agcggctcga cctcgacccg
     7741 cttgtcgaag gagatccacg gcacctcgcg aaagacgacg ttcaggatca cgacgcgctc
     7801 gtgcagcacc ttgttgtgct tcaggttgtg cagcagcgcc tgcggcacgc gctccggatc
     7861 gccgaccgcg tagaccgccg tgccggcgac gcgcctgacg tcgtcgcgca gcagcaggtc
     7921 gatgaagccc ttcacttccg ggtccgcgcg gtggatgtgc tcgaacagca gttcgcgccc
     7981 gcgcttccac gtcgccatca ccgcgaacag caccagcccg agcgcgagcg tgaaccagcc
     8041 gccgtcgaag aacttcgccg agcacgcggt gacgagcacc gcatcgagca ggatgaagaa
     8101 cgcagtggcc gcgagcgcga gccagagcgg gtaacgccac ccatgacgca cgacgaagaa
     8161 cgtcaggaac gtggtgacca gcatcatcag cgtgactgcg atgccgtagg caccggccag
     8221 gttggacgac gagcggaagc cggtcgtgac gaccagcacc gccgcgagca gcatccagtt
     8281 gacggcgggc atgtagatct gccccatttc cttcgccgac gtgtacgtaa tcgccatgcg
     8341 cggcaggaag ccgagatgga tcgcctgctg cgtcatcgaa ta
//
LOCUS       DBNHBY010000249         3589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1503672, whole genome shotgun sequence.
ACCESSION   DBNHBY010000249 DBNHBY010000000
VERSION     DBNHBY010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1503672"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16HB_06145"
     CDS             complement(<1..245)
                     /locus_tag="AC16HB_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014273.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR5606406.1"
                     /translation="MNVKRIRCADIKPDRGASRYLPHEIEVLADSIRRHGILRPVLLR
                     AVADGYVIVHGVRRWHAALLLGLKEIPAFIVETLSGH"
     gene            complement(438..1259)
                     /locus_tag="AC16HB_06150"
     CDS             complement(438..1259)
                     /locus_tag="AC16HB_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR5606407.1"
                     /translation="MFEFIGRAIAGYLSKPIGKPGGGSAEAALVADALAPGDVLLIEG
                     NTRMSAAIKYLTQSTWSHAALFVGPRPELPVVEGEAATLIEADMLEGVRAVPLSCYIN
                     FRVRICRPVRLTTEDCERLILYMVERLGYRYDLKNLLDLARYLLPLPVPARLRRRLIA
                     FGSGDPTRAICSTLIAQAFGAVRYPILPIISRKHDGNPGRADQVRELWRIRHHSLYVP
                     RDFDISPFFEVVKPRIAKATFDYQCIPWSEGNTVITGDAPPTDFPTILPMGDTPQ"
     gene            complement(1365..2171)
                     /locus_tag="AC16HB_06155"
     CDS             complement(1365..2171)
                     /locus_tag="AC16HB_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR5606408.1"
                     /translation="MSARAVARSPSRGWLAAVLIAGIAALAWTALVVFSAGPYARYLD
                     HGRWTDLPLLGAWCTPLAQGEAAALLLFHAAAWLLMIAAMMLPTVLPLVGLVAQMAFG
                     RRDASGAVALVVLGYVIIWSAFGVLAFGIDAAVRGVAEESPWLAAHGRWLGVAVLAGA
                     GAFQFSALKYRCLDRCRTPFGFINERWSGRTPRRDALRIGLDHGLFCVGCCWALMLVM
                     FVVGMSNLGWMLVLAALMAAEKNLPIGRRLAAPVGIALLAWAALLAAGAA"
     gene            complement(2168..2770)
                     /locus_tag="AC16HB_06160"
     CDS             complement(2168..2770)
                     /locus_tag="AC16HB_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR5606409.1"
                     /translation="MGYHVEGRLLEVCNCRVLCPCWIGEDPDFGTCDTIVAYRFDKGT
                     IDGVDVSGKTIALIAHVPGNILQGNWRAAVYLDDSVSPQQEQAILNLYTGKLGGPVAD
                     LVKLVGEIVSVEKVPIQFDVHEGRGTLKVGSNGYAELEPYQGATGSNTTLSDTIFSTV
                     PGAPVYVGKSPRYWSKNDKLGINLDIKGHNALQSTFRFDA"
     gene            3126..3410
                     /locus_tag="AC16HB_06165"
     CDS             3126..3410
                     /locus_tag="AC16HB_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606410.1"
                     /translation="MSVITIRAAAVNPAATPWSNGHGRRYAPIRLGVAMKFTIWLVVE
                     LALMPAFVLFAGLSVAGALKSPVEYAVTGAFLVAALIAGAMALRALRRDD"
BASE COUNT          600 a         1199 c         1207 g          583 t
ORIGIN      
        1 tcgtggccgc tcagcgtctc gacgatgaac gccggaatct ccttcagccc gagcagcagt
       61 gcggcatgcc agcggcgcac gccgtgcacg atcacgtagc cgtcggccac ggcgcgcagc
      121 agcaccggcc gcaggatgcc gtggcggcgg atgctgtcgg cgaggacttc gatttcgtgg
      181 ggcagatagc ggctggcacc gcggtccggc ttgatgtcgg cacagcggat tcgcttcacg
      241 ttcatgctcg ctcccggttc agatcgaatg gcgttgtgct ttagaccgag tttgccggca
      301 acatcgttaa gcgaagatga aattcgcatg ccgcattcgc gcgaagacgt ttcgcgcaac
      361 ccgaacacgc gtcgcgtgaa acgggacttg tcctcaccgc gcgctgttgg agtcgccgcg
      421 ctgcagatcc gcgtcggtca ctgcggcgtg tcacccatcg gcaggatggt cgggaagtcc
      481 gtcggtggcg cgtcgcccgt gatgaccgta ttgccctcgc tccacgggat gcactggtag
      541 tcgaaggtcg ccttcgcgat gcgcggcttg accacctcga agaagggcga gatgtcgaag
      601 tcgcgcggca cgtacaggct gtgatggcgg atgcgccaca gttcgcgcac ctggtcggcg
      661 cggcccggat tgccatcgtg cttgcgcgag atgatcggca ggatcggata gcggaccgca
      721 ccgaacgcct gcgcgatcag cgtcgagcag atcgcgcgtg tcgggtcgcc gctgccgaag
      781 gcgatcagcc ggcggcgcag gcgcgcgggc acgggcagcg gcagcaggta gcgcgccaga
      841 tcgagcaggt tcttcaggtc gtaccggtaa ccgagccgct cgaccatgta aaggatcagg
      901 cgctcgcaat cctccgtcgt cagccggacc ggtcgacaga tacgcacccg gaagttgatg
      961 tagcaggaca gcggcacggc gcgcacgcct tccagcatgt cggcctcgat cagcgtggcc
     1021 gcctcgcctt cgacgaccgg cagttcaggg cgcggcccga cgaacagcgc cgcgtgcgac
     1081 caggtcgact gtgtcaggta cttgatcgcc gcgctcatgc gcgtgttgcc ttcgatcagc
     1141 agcacgtcgc ccggcgcaag ggcgtcggcg accagcgccg cctcggcgct gccgccgccc
     1201 ggcttcccga ttggcttcga cagatagccg gcgatcgccc ggccgatgaa ctcgaacacc
     1261 gttccgatgg ggaggcatga aagtgacgac cgactatatc catttggcgc agcgcgtcac
     1321 gcggccgttc gtatggtccg cggtagccgc tacggacggt gcggctatgc cgcgccagcc
     1381 gcgagcagcg ccgcccaggc gagcagcgcg atgccgactg gcgccgcgag gcgacgcccg
     1441 atcggcaggt tcttctcggc cgccatcagc gcggccagga ccagcatcca gccgaggttg
     1501 ctcatcccga ccacgaacat cacgagcatc agcgcccagc agcagccgac gcagaacagg
     1561 ccgtgatcca ggccgatgcg cagcgcgtcg cgccgcggcg tgcggccgct ccagcgctcg
     1621 ttgatgaaac cgaacggcgt gcgacagcgg tcgaggcagc ggtacttcag tgcgctgaac
     1681 tggaacgcgc ccgcaccggc gagcacggcc acgccgagcc agcggccgtg cgcggcgagc
     1741 cagggcgact cttcggcgac cccgcgcacc gccgcgtcga tcccgaacgc gagcacgccg
     1801 aacgcgctcc agatgatcac gtagccaagg acgaccagcg caaccgcgcc acttgcatcg
     1861 cggcggccga atgccatctg cgccaccagg ccgacgagcg gcagcacggt cggcagcatc
     1921 atcgccgcga tcatcagcag ccacgcggcg gcatggaaca gcagcagcgc ggccgcttca
     1981 ccctgcgcca gcggtgtgca ccacgcgccg agcagcggca gatcggtcca gcgaccgtgg
     2041 tccaggtagc gcgcgtacgg ccccgcgctg aacacgacaa gcgcggtcca cgcgagcgcg
     2101 gcgatcccgg cgatcaggac cgccgccagc cagccgcgcg acgggctccg agcgacggcg
     2161 cgtgcgctca cgcgtcgaag cggaacgtgc tttgcagcgc gttgtgcccc ttgatgtcga
     2221 ggttgatgcc gagtttgtcg ttcttcgacc agtagcgcgg gctcttgccc acataaacgg
     2281 gcgcgcccgg caccgtcgag aagatcgtgt cggagagcgt cgtattcgat ccggttgcgc
     2341 cctgatacgg ctcgagttcg gcgtagccgt tgctgccgac cttcagcgtg cccctgccct
     2401 cgtgcacgtc gaactggatc ggcaccttct cgaccgacac gatttcgccc accagcttga
     2461 cgaggtcagc gaccggtcca ccgagcttgc ccgtatagag gttcaggatc gcctgctcct
     2521 gctggggcga cacgctgtcg tcgaggtaga cggccgcccg ccagttgccc tgcaggatgt
     2581 tgccgggaac gtgggcgatc agcgcgatgg tcttgccgga cacatcgaca ccgtcgatcg
     2641 tgcccttgtc gaagcggtaa gcaacgatcg tgtcgcaggt tccgaaatcc gggtcctcgc
     2701 caatccagca aggacacagg acccggcagt tgcagacttc cagcagccgt ccttccacgt
     2761 gatagcccat cgtcgtctcc tgttcggttg agggtcgaat cgcacctgcg tagagcacct
     2821 gcggccgctg ggcggcatac gtcttgtgac acttgcttgc gagctatccg gcgcatcgtc
     2881 cacgttgaag cgagcggcaa tcgtcacgaa acgctcgtcg gcaatcttcg atcgtgggcg
     2941 tcgcccgcgc cctcaggcgc tgaagcgaaa cgtgctctga atcgcgttga gtgttgactc
     3001 cttatcgctc cgacagcgca cggccgcgcc tggcgcaagt gtcatgcgcc gagtgggcgg
     3061 gtctattcct gcacccgtcg ccgtgcaatc ccaagaatcg atcacgccgt gcggggccct
     3121 cgagcgtgag cgtgatcacg atccgcgcgg cggccgtaaa ccccgcggcg actccgtggt
     3181 cgaatggtca cggacggcgg tacgcgccga tccgcttggg agtcgcgatg aaattcacca
     3241 tctggctggt ggtggaattg gcgttgatgc cggcgttcgt gctgttcgcc gggctgagcg
     3301 tggccggcgc attgaagtcg ccggtggagt acgccgtcac gggcgccttt ctcgtcgcgg
     3361 cgctgatcgc aggcgcgatg gcgctgcgcg cgctgcgccg cgacgattaa cgctccctta
     3421 attcgtcgta cgcagaatca cttaaagcgg gttgcgggag gcagggcgtg cgtgtactgc
     3481 tggtcgatga ctaccggatg atcggcgaca acctgagaag cgcgctgcgc cagtcgggct
     3541 acgcggtcga ctgggtgcgc gacggtgccg ccgccgacac ggcgctgtc
//
LOCUS       DBNHBY010000250         2905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1706739, whole genome shotgun sequence.
ACCESSION   DBNHBY010000250 DBNHBY010000000
VERSION     DBNHBY010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1706739"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16HB_06170"
     CDS             <1..357
                     /locus_tag="AC16HB_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5606411.1"
                     /translation="FSSIEEIAYVPQSEIASIEEFDEEIVQELRNRARDVLLTQAIAS
                     EETLEKAPAADLLEVEGMDAQLAAELARHGIVTREDLAEQAVDDLVEIPDLDEKRAGE
                     LILAARKHWFEQGQQA"
     gene            374..1105
                     /locus_tag="AC16HB_06175"
     CDS             374..1105
                     /locus_tag="AC16HB_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HIR5606412.1"
                     /translation="MAEVTVTQFAEVLKVPVERLLVQLGEAGIKVGGANDIISDEAKM
                     ELLTHLRRAHGRQEDAAAPRKITLQRKSQQEIKLASTQGRARTVSVEVRRKKTYLNRS
                     VIEEEQRQRQEEVDKQRPLPPEETDRLEQERIEAERRDAEQRERERLEEESRRAEEES
                     RRRAEADARAAAEQLARERTERTERDRAEAERRVLEQQRAALAKARPAETAPTRPAPG
                     AAPSTKYGRQELHVSANASARFKKK"
     gene            1112..2905
                     /gene="infB"
                     /locus_tag="AC16HB_06180"
                     /pseudo
     CDS             1112..2905
                     /gene="infB"
                     /locus_tag="AC16HB_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915210.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
BASE COUNT          529 a          967 c         1045 g          364 t
ORIGIN      
        1 ttctccagca tcgaggaaat cgcctacgtg ccgcagtccg agatcgcctc gatcgaggaa
       61 ttcgacgagg agatcgtgca ggagctgcgc aaccgcgccc gtgacgtgct gctgacgcag
      121 gcgatcgcga gcgaggagac gctcgagaag gccccggcgg ccgatctgct cgaggtcgag
      181 ggcatggacg cgcagctcgc tgcggaactg gcacgccacg gcatcgtgac gcgcgaggac
      241 ctcgccgagc aggccgtgga cgacctcgtc gaaatcccgg acctggacga gaagcgcgcg
      301 ggcgagctga tcctcgcggc gcgcaagcac tggttcgagc agggccagca ggcgtgagcg
      361 taaggagcac tacatggcgg aagtgacggt cacgcagttt gccgaggtcc tgaaggttcc
      421 ggtcgagcgc ctgctggtgc agctcggcga agccggcatc aaggtcggcg gcgccaacga
      481 catcatcagc gacgaagcca agatggaact cctgacgcac ctgcgccgcg cgcacggtcg
      541 gcaggaagac gcggctgcac cgcgcaagat cacgctgcag cgcaagtcgc agcaggagat
      601 caagctcgcc tcgacgcagg gccgggcccg cacggtctcg gtcgaggtcc gccgcaagaa
      661 gacgtacctc aaccgctccg tcatcgagga agaacagcgg cagcggcagg aagaggtcga
      721 caagcagcgt cccctgcctc ccgaggaaac ggaccgcctc gagcaggagc gcatcgaggc
      781 cgagcgtcgc gacgccgagc agcgcgagcg cgagcgcctc gaggaggaga gccgtcgcgc
      841 cgaggaagag tcgcgccgtc gcgcagaggc cgatgcccgt gcggccgccg agcagctggc
      901 gcgcgaacgc accgaacgca ccgaacgcga ccgcgcggag gccgaacgcc gcgtgctcga
      961 acagcagcgc gccgcgctgg ccaaggcgcg gccggccgaa accgccccga cgcgacctgc
     1021 gccgggcgcc gcgccgtcca cgaagtatgg ccgtcaggag ctgcacgtct ccgccaacgc
     1081 gagcgcgcgc ttcaagaaga agtagcaggc accgggtggt cgtttccgcc gctcgggcca
     1141 ggtgcaggtc gaggcccgcc acggcttcgt cgagcccacg gcgccggtca agcgcgaggt
     1201 gcagctgccc gagacgatca cggtcggcga actcgccaac cgcatggcca tcaaggccaa
     1261 cgaagtcatc aagacgatga tgaagatggg cgtgatggcc accatcaacc agcccatcga
     1321 ccaggacacg gcgcagctcg tggtcgagga gttcggccac accgcggtcc gcctcaagga
     1381 ggacgtgatt gaggagcagc tgctcgcgca gcaggccgac gtcgagctgc tgccgcggcc
     1441 gccggtggtc acgatcatgg gccacgtcga ccacggcaag acgtcgctgc tcgactacat
     1501 ccgccgcacc aaggtcgcgg cgggcgaagc cggtggcatc acgcagcaca tcggcgccta
     1561 ccaggtcgag acgccgcgcg gcctgatcac gttcatcgac accccgggcc acgccgcgtt
     1621 caccgcgatg cgcgcccgcg gcgcgcacgt caccgacatc gtgatcctgg tggtcgcggc
     1681 cgacgacggc gtcatgccgc agacgatcga ggcgatccag cacgcgcgcg cggccgaggt
     1741 gccgatcgtc gtcgcggtca acaagatcga caagcacggc gcggatcccg agcgggtccg
     1801 caacgacctc ggcaagcaca acgtgattcc cgaggcctgg ggcggcgacg tgatgttcgt
     1861 gaacgtgtcg gccaagacgg gcgagggcgt cgattcgctg ctcgagtccg tgctgctgca
     1921 ggccgaagtg ctcgacctca aggcgccacg ccagggcatg gcggcgggcg tcgtgctcga
     1981 atcgagcatc gagaagggcc gtggcccggt cgcgaccgtg ctggtcaagc gcggcacgtt
     2041 gcggaccggc gacccgatca tcgccggcca ggagttcggc cgtgtccgcg cgctgttcga
     2101 cgcgctcggc aagcaggtgc aggaggccgg tccggccgtg ccggtgcagg tgctcggcct
     2161 gtccgatgcg cccaacgccg gcgacgactt cctcgcagtc gagagcgagc gcaaggcgcg
     2221 cgaagttgcg ctgtatcgcc agggcaagtt ccgcgacgtg cgcctcgccg gtgcggcgaa
     2281 gaaggtcgag gacgtcttct cgcagatggg cgaggcggcc ggccaggtgg tcaacctcat
     2341 cgtcaaggcc gacgtgcagg gcagcgccga agcgctgcgc gactcactgt cgaagatcgg
     2401 cacggacgag gtgtcggtca aggtcatcgc gagcggcgtc ggcgggatca ccgaatccga
     2461 cgtgtcgctg gcggcggcct cgcgtgcccg catcatcggc ttcaacgtcc gcgccgacgg
     2521 cgcggcgcgc ggtgcgatga aggagtcggg ggtcgaggtc aactactaca gcatcatcta
     2581 cgaggcgctg gacgacgtgc gcagcctggt ctcgggcatg ctcaagcccg aggtcaagga
     2641 gcagatcgtc ggcctggccg aggtgcgcga ggtgttccgc tcgagcaagt tcggcacggt
     2701 ggccggctgc ctcgtggtgg aaggcttcac gaagcgcggc aacccggtgc gcgtgctgcg
     2761 cgacaacgtc gtgatccacc agggcgaact cgactcgctg cggcgcttca aggacgacgt
     2821 cggcgaggtc cgtgccggca ccgagtgcgg catcggcgtg cgcaactaca acgacatccg
     2881 cgcgggcgac cagatcgagt gcttc
//
LOCUS       DBNHBY010000251         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_623444, whole genome shotgun sequence.
ACCESSION   DBNHBY010000251 DBNHBY010000000
VERSION     DBNHBY010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_623444"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="AC16HB_06185"
     CDS             <1..1669
                     /locus_tag="AC16HB_06185"
                     /inference="COORDINATES: protein motif:HMM:NF036590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HIR5606413.1"
                     /translation="EFVADGLPDEASRALRRIDDVRTLRATQFPEDAGPMAHPVRPDS
                     YQEIANFYTATVYEKGAEVVRMLHALLGREGFRRGLESYLQRHDGQAVTCDDFVAALA
                     DANDRDLSQFSRWYAQAGTPRVSVASSFDAGARACTLTFTQSCPPTPSQADKAPFHMP
                     FAVGLIGADGRDLPLRLEGEVGAPTTRVLELTQPTQMFRFVDVDAAPVPSLARGFSAP
                     VIVDYPYTDTELAFLTRHDSDPFNRWEAAQRLACMRLLALVDAVEAGRPLALDGALTD
                     LHAQILDDAALAPAFKAQALQLPTESFLAEQRAMVDPTAIRSARQFARRELGSELAPR
                     WRALYAQLASSAAYRPDPAEAGRRALRNLALGLLVDSGNADALVLARAQFDQSDNLTD
                     RLAALAAIVNSTAPFKTDILARVAFDWRAAPLLMNKWFQLQATAIALPGEAPVLPRVR
                     TLMKHPAFSISNPNNVYALILGFCAHNPAEFHRDDGTGYEFWIEQVLRLDRLNPTVAA
                     RVARALDRWRKFTPSRGRLMRNALLEVAGHASLSRDVREIVTKALAG"
     gene            1675..2112
                     /locus_tag="AC16HB_06190"
     CDS             1675..2112
                     /locus_tag="AC16HB_06190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5606414.1"
                     /translation="MLSWLLVVLQFALIGLLIVTTQPLATPASNAAAAILLAAGCAVG
                     IAALRANRPGNFNVRPELKSGARLVTHGIYRYVRHPMYLGVLLAMAAAIAADPRLSRC
                     ALWAALLAVLLAKLGREERNLLAAFPGYSDYAARTRRLIPGLY"
     gene            2209..>2515
                     /locus_tag="AC16HB_06195"
     CDS             2209..>2515
                     /locus_tag="AC16HB_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606415.1"
                     /translation="MKLRSALAFVALAPTLLGTAAAQSLLMSPEWAQAACEAWNKDAV
                     LTDQLAESGWVKNDKGRGFKVMHLFRKDCGAAPTAELRVALKDGKALCVYGGKVETAK
                     "
BASE COUNT          371 a          890 c          853 g          401 t
ORIGIN      
        1 ggagttcgtc gccgacggcc tgcccgatga agccagccgc gcgctgcgca ggatcgacga
       61 cgtacgcacc ttgcgcgcga cgcaattccc ggaggacgcc gggccgatgg cgcacccggt
      121 gcgacccgat tcgtaccagg agatcgccaa cttctacacc gcgaccgtct acgaaaaggg
      181 cgccgaggtc gtacgcatgc tgcatgcgct gctcgggcgc gagggctttc ggcgcggact
      241 cgagtcgtac ttgcagcgcc acgacgggca ggcagtcacg tgcgacgact tcgtcgctgc
      301 gctggcggac gccaacgacc gtgacctgtc gcagttctcg cgctggtacg cgcaggcggg
      361 cacgccgcgc gtcagcgtcg catcgagctt cgacgccggc gcgcgcgcct gcacgttgac
      421 gttcacgcag agttgccccc ccaccccaag ccaggccgac aaggcgccgt ttcacatgcc
      481 gttcgccgtc gggctgatcg gcgcggacgg gcgcgacctg ccgctgcgtc tggaaggcga
      541 ggtcggcgcc ccgaccacgc gcgtactgga gctgacgcag ccgacgcaga tgttccgttt
      601 cgtcgacgtc gatgcggcgc cagtgccgtc gcttgcgcgt ggcttttctg cgcccgtcat
      661 cgtcgactac ccgtacaccg acaccgagct ggcatttctc acgcgccacg acagcgatcc
      721 attcaatcgc tgggaggccg cgcagcggct ggcgtgcatg cgcctgctcg cgctggtcga
      781 cgcggtcgaa gccgggcgac cgctcgccct cgacggcgcg ctgaccgacc tgcatgcgca
      841 gatcctcgac gatgcggcgc tggcgccggc gttcaaggcg caggcgctgc agctgccgac
      901 ggaatcgttc ctcgccgagc agcgcgcgat ggtcgatccg acggcgatcc gctccgcgcg
      961 ccagttcgcc cgccgcgagc tcgggagcga gctggcgccg cgctggcgcg cgctgtatgc
     1021 gcaactcgcg agcagcgccg cgtaccggcc cgatccggcg gaagccgggc ggcgtgcgct
     1081 gcgcaatctt gcgctcggtc tgctggtcga cagcggcaac gccgacgcgc tggtgctggc
     1141 gcgcgcgcag ttcgaccagt ccgacaacct gaccgaccgg ttggccgcgc tggctgcgat
     1201 cgtcaacagc accgcgccat tcaagaccga catcctggcg cgcgtcgcgt tcgactggcg
     1261 cgccgcgccg ctgctgatga acaagtggtt ccagctacag gccacggcga tcgcgctgcc
     1321 cggcgaagcg cccgtcctgc cgcgcgtgcg caccttgatg aagcatccgg cattctcgat
     1381 cagcaatccc aataacgtct acgcgctgat tctcgggttc tgcgcccaca atccggcgga
     1441 attccatcgc gacgacggca ccggctacga gttctggatc gagcaggtgc tgcgtctcga
     1501 ccgcctgaat ccgaccgtgg cagcgcgcgt ggcgcgcgcg ctcgaccgct ggcgcaagtt
     1561 cacgccaagc cgcggccggc tgatgcgcaa cgcgttgctc gaagtcgcgg gccacgcatc
     1621 gctgtcgcgc gacgtgcgcg agatcgtgac caaggcgctc gcgggttgac gcgcatgctg
     1681 tcgtggttgc tggtcgtgct tcagttcgcg ctgatcgggc tgctgatcgt aaccacgcag
     1741 ccgctcgcga caccggcatc gaacgcggcc gccgcgatcc tgctcgccgc cggctgcgcg
     1801 gtcggcatcg ctgccctgcg tgccaaccgt ccgggcaact tcaacgtccg gcccgaactg
     1861 aaatccggcg cgcggctcgt cacgcacggc atctatcgtt acgtgcgcca tccgatgtat
     1921 ctcggcgtgc tgctcgcgat ggcggcggcg atcgccgccg atccgcgcct gtcgcgctgc
     1981 gcgctgtggg cagcgctgct ggcggtgctg ctcgccaagc tcgggcgcga ggagcgcaat
     2041 ctgctggcgg catttccggg atattcggac tatgcggcgc gcacgcggcg gttgattccc
     2101 ggcttgtact gagtccaaaa gcggacgcct gtgtgagctt cgtcacacag atcgagcgtg
     2161 ccgcgccgat acgcttgcgg tcgaacccaa cgacgcccgg gacctgcaat gaagctgcgc
     2221 tccgctctcg cctttgtcgc cctcgcgccc acgctgctcg gaaccgccgc tgcgcagtcg
     2281 ctgctgatgt cgcccgaatg ggctcaagcc gcctgcgagg cctggaacaa ggacgccgtg
     2341 ctgaccgatc agctcgccga gtcgggctgg gtgaagaacg acaagggtcg cggcttcaag
     2401 gtcatgcacc tcttccgcaa ggactgcggc gcggcgccga ccgcggagct gcgcgtcgcg
     2461 ctgaaggacg gcaaggcgct gtgcgtgtat ggcggcaagg tcgagaccgc gaagc
//
LOCUS       DBNHBY010000252         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_962397, whole genome shotgun sequence.
ACCESSION   DBNHBY010000252 DBNHBY010000000
VERSION     DBNHBY010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_962397"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="AC16HB_06200"
     CDS             complement(<1..241)
                     /locus_tag="AC16HB_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5606416.1"
                     /translation="MAGRLRGKVALVTAAGQGMGRAGALAFAREGAQVIATDLKPELL
                     RTLEGTPAVRTRALDVRDHAAVHALVEEIGAVDILF"
     gene            412..1110
                     /locus_tag="AC16HB_06205"
     CDS             412..1110
                     /locus_tag="AC16HB_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5606417.1"
                     /translation="MPLQAVEARRLYRQIADQLITLIRGGEYRPGARLPAERDLARQL
                     GVSRPSVREALIALEVEGYVEVRVGAGVYVRNRAGVASARAALPPDSGPFELIEARRI
                     VESECAALAARKATPAQLRQLKAALNSMRRDRRRNVVPLEGDRLFHERISAASGNSAL
                     AMLVGTLWVQRTGPLFLQLEHHFDTPALWSAAISEHETVLHAIERHDAAAARTAMRRH
                     LDRAAMRFKRSFGK"
     gene            1114..>1655
                     /locus_tag="AC16HB_06210"
     CDS             1114..>1655
                     /locus_tag="AC16HB_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5606418.1"
                     /translation="MDEARERDDAPVMTDDGHFDVHDAPVDLGVYRIEDWVALAFFWV
                     LALVIFLQFFTRYALNDSASWTEEIARYLLICTVFIGAAISVRKNNHVQVDFFYRVLP
                     RPLMRLLSTLVDLARIGFFGYAVVLTYSLMQRIGRQPMSIIDWPIGIVYAVVLGGFVL
                     MTWRAVQVAALNWRRGASVL"
BASE COUNT          233 a          557 c          594 g          271 t
ORIGIN      
        1 tgaacaggat gtcgaccgcg ccgatctcct cgaccagcgc gtgcaccgcg gcgtggtcgc
       61 gcacgtcgag tgcgcgggtg cggacggccg gcgtgccttc gagcgtgcgc agcagctcgg
      121 gcttcagatc ggtggcgatc acctgcgcgc cctcgcgcgc gaatgccagc gcgccggcgc
      181 gtcccatgcc ctggccggcc gcggtcacga gcgcgacctt gccccgcaaa cgtcctgcca
      241 tcgaatctcc cttgtgatgc ctgccgcgcg cgggtgcgtt gacactgcgg cggcgccatg
      301 cgaagctgcc ccgccaattg gcctgaccac tttaccagcg cgcggcagtg taagagacgg
      361 ccccggggct tgtccatcgc gcatcgaacc cagccagaat ccgctcctgc catgccattg
      421 caagccgtcg aggcgcggcg actgtatcgc cagatcgccg atcagctgat cacgctgatc
      481 cgcggcggcg agtaccggcc gggtgcgcgc ctgccggcag agcgcgacct ggcgcgccag
      541 ctcggcgtca gccgaccctc ggtgcgcgag gcgttgatcg cgctcgaggt cgagggctat
      601 gtcgaggtgc gggtcggcgc cggcgtgtac gtgaggaacc gcgctggcgt ggcgagtgcg
      661 cgcgcggcgc tgccgcccga ttcgggtccg ttcgagctga tcgaggcgcg ccgcatcgtc
      721 gaatccgaat gcgcggcgct cgccgcgcgc aaggcgacgc ccgcgcaact gcggcagttg
      781 aaggcggcgc tgaattcgat gcgccgggac cgccggcgca acgtcgtgcc gctcgagggc
      841 gaccgtctgt ttcacgagcg gatcagcgcc gcgagcggca acagcgcgct ggcgatgctg
      901 gtcggcacgc tgtgggtgca gcggaccggg ccgctgttcc tgcagctcga gcatcacttc
      961 gacacgccgg ccctgtggtc ggccgcgatc agcgagcacg aaacggtgct gcacgcgatc
     1021 gaacgccacg acgccgcggc cgcgcggacg gcgatgaggc gccacctcga tcgcgcggcg
     1081 atgcgcttca agcgcagctt cggaaagtag cgcatggacg aggcacggga gcgcgacgac
     1141 gcgccggtca tgaccgacga cgggcacttc gacgtgcacg acgcgccggt cgaccttggc
     1201 gtgtaccgga tcgaggactg ggtcgcgctc gcgttcttct gggtgctcgc gctggtgatc
     1261 ttcctgcagt tcttcacccg ctacgcgctg aacgactcgg cgtcctggac cgaggagatc
     1321 gcgcggtatc tcttgatctg caccgtgttc atcggcgcgg cgatcagcgt gcgcaagaac
     1381 aaccacgtac aggtcgactt cttctaccgc gtgctgccgc ggccgctgat gcgcctgctg
     1441 tcgacgctgg tcgatctggc gcgcatcggc ttcttcggtt acgcggtggt tctgacttac
     1501 tcgctgatgc agcgcatcgg gcggcagccg atgtcgatca tcgactggcc gatcggcatc
     1561 gtgtacgcgg tcgtgctggg gggcttcgtg ctgatgacgt ggcgcgcagt gcaggtggcg
     1621 gcactgaact ggcggcgcgg cgcgtcggtg ctgga
//
LOCUS       DBNHBY010000253         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1639160, whole genome shotgun sequence.
ACCESSION   DBNHBY010000253 DBNHBY010000000
VERSION     DBNHBY010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1639160"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1300)
                     /locus_tag="AC16HB_06215"
     CDS             complement(125..1300)
                     /locus_tag="AC16HB_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR5606419.1"
                     /translation="MAVISKDTAFLGYRRENRRVGVRNHVLILPLDDLSNAAAEAVAH
                     NIKGALAIPHPYGRLQFGADLELHFRTLIGAGCNPNVAAVVVIGIEDSWTRKVVDGIA
                     VTGKPVTGFGIEGHGDHDTIMRASRAAREYVQWASEKQREPCNIRELWVSTKCGESDT
                     TSGCGANPTVGNAFDKLHALGSTLCFGETTELTGGEHIVAARCVNDAVRERFMFMFNR
                     YQDVINRHKTSDLSDSQPTKGNIAGGLTTIEEKALGNIQKIGKKCTVDGVLDKAEMPT
                     HPGLWFMDSSSAAAEMVTLCAASGFAVHFFPTGQCNVIGNPILPVIKICANPRTVRLM
                     SEHIDVDTSGLLQREITLDQAGDKLLECMLRTANGRLTAAEALGHREFVLTRLYESA"
     gene            complement(1313..1594)
                     /locus_tag="AC16HB_06220"
     CDS             complement(1313..1594)
                     /locus_tag="AC16HB_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR5606420.1"
                     /translation="MIHFVLHDRKDTVAVVVVEGVQAGMQLTGWIMDEDRTATVQARQ
                     DIPIGHKIALTDMNPGDTVWKYGIDMGKVVAPIRAGEHAHVHNIKTKRW"
     gene            1706..2512
                     /locus_tag="AC16HB_06225"
     CDS             1706..2512
                     /locus_tag="AC16HB_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5606421.1"
                     /translation="MPTAAAARNHVSATPGRHAPALRAIAPEAADMPLYRVVKRALLQ
                     VIEDGTYAPGAALPNETALATTFGVSVGTLRHAVDELAADHILVRRQGRGTFVASHNA
                     DRFVFQFFHIERADGLREAPLVELIGFERTRLDEEAAQALGSRPGEPGLAIETRLSLQ
                     GRAVVHDRIALPAALFRGLTEKRFRDRPSTIYHLYQIEFGITVVRAHERLRAAAANRV
                     AARLLGLNHGAPVLQVRRTALTFGDKPVEYRVSAVNTAHHDYVNLLTRPT"
     gene            2509..3303
                     /locus_tag="AC16HB_06230"
     CDS             2509..3303
                     /locus_tag="AC16HB_06230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5606422.1"
                     /translation="MSGIKLARALEVAQTAVRAAGRFLLDSRARIGSAALEGRAPREV
                     TANIEREAEALIRSRIGAVFPEHGFVGADLGGTFDARRAQWLVDALDGTDNYLHGYPQ
                     YAVSLALQHQGEAVLGVIYDPHRDELFSATRGQGAYCNGSRLACSMRAAPEEALVATM
                     FPPPNSECMPRFLEEFARVTNGCGGVRRSGALALDLAYLAAGRVDAFWAHDVGAWDAA
                     AAIILLRESGARVESLDNGPLLASRSLLAAAPSLLAPTRALLAGRT"
BASE COUNT          498 a         1176 c         1190 g          574 t
ORIGIN      
        1 cgctgcggag cgtcgccgat gatagccggc cacgctgcgg tgacagcttc gcgcagaccg
       61 cccacgcctc gggattcgcg cccgttgggt cggcgcagcg cgcaacgccc ggtcgtcccg
      121 gaggtcaggc cgactcgtac agccgggtca gcacgaactc gcgatggccg agcgcctcgg
      181 ccgcggtcag ccgcccgttc gcggtgcgca gcatgcactc gagcagcttg tcacccgcct
      241 ggtcgagcgt gatttcacgc tgcagcaggc ccgaagtgtc gacgtcgata tgctcgctca
      301 tcagccgcac cgtgcgcggg ttggcgcaga tcttgatcac cggcaggatc ggattgccga
      361 tcacgttgca ctggccggtg gggaagaaat gcactgcgaa tccgctggcc gcgcatagcg
      421 tcaccatctc ggccgcagcc gacgacgaat ccatgaacca cagaccggga tgggtcggca
      481 tctcggcctt gtcgagcacg ccgtcgaccg tgcatttctt gccgatcttc tggatgttgc
      541 cgagcgcctt ctcctcgatc gtcgtcagac cgccggcgat gttgcccttg gtcggctgcg
      601 agtcggaaag atccgaagtc ttgtggcggt tgatcacgtc ctggtagcgg ttgaacatga
      661 acatgaaacg ttcgcgcacg gcgtcgttga cgcagcgcgc ggcgacgatg tgttcgccgc
      721 cggtcaattc cgtcgtctcc ccgaagcaca gggtgctgcc gagcgcatgc agcttgtcga
      781 acgcgttgcc cacggtggga ttggcgccgc agccgctggt ggtgtcgctc tcgccgcact
      841 tggtcgacac ccacagctcg cggatgttgc aaggctcgcg ctgcttctcg ctcgcccatt
      901 gcacgtattc gcgcgctgct cggctcgcgc gcatgatcgt gtcgtggtcg ccgtggccct
      961 cgatgccgaa gccggtgacc ggcttgcccg tgacggcgat gccgtcgacc accttcctgg
     1021 tccagctgtc ctcgatgccg atcacgacca ccgccgccac gttcgggttg cagccggcgc
     1081 cgatcagcgt acggaaatgc agttcgaggt cggcgccgaa ctgcagccgc ccgtaagggt
     1141 gcgggatcgc gagcgccccc ttgatgttgt gcgcgaccgc ctctgccgcc gcatttgaca
     1201 ggtcgtcgag cggcagaatg agcacgtggt tgcgtacgcc gacgcgtctg ttctcgcgcc
     1261 ggtagcccag gaacgcggtg tccttcgaga tgactgccat cgcgatttcc tcctaccagc
     1321 gcttggtctt gatgttgtgc acgtgggcgt gctcgccggc cctgatcggc gcgaccacct
     1381 tgcccatgtc gatgccgtac ttccacacgg tgtccccggg attcatgtcg gtgagcgcga
     1441 tcttgtggcc gatcgggatg tcctggcgcg cctgcacggt cgccgtcctg tcctcgtcca
     1501 tgatccagcc ggtgagctgc atgccggcct gcacgccctc caccaccacc accgcgaccg
     1561 tgtccttgcg gtcgtgcagt acgaaatgaa tcatcgcgtc ctccggcgct gcggatgccg
     1621 gggactgtag tcagcgcccg acagcctgtc aaacaagtca tatgaatcac ttgcatgaca
     1681 agcatggcaa ggcgacaatc ggtgcatgcc gactgccgcc gccgcgcgca accacgtgag
     1741 cgccacgccg gggcgccacg cgccggcgct gcgcgcgatc gcgccggagg cggccgacat
     1801 gccgctgtac cgcgtcgtca agcgggcgct gctgcaggtg atcgaggacg gcacctacgc
     1861 gccgggggcg gcgctgccga acgagaccgc gctggcgacg actttcggcg tctccgtcgg
     1921 cacgctgcgg cacgcggtcg acgaactcgc ggcggaccat atcctggtgc ggcggcaggg
     1981 gcgcggcacg ttcgtcgcca gccataacgc cgaccgcttc gtgttccagt tctttcacat
     2041 cgagcgcgcc gacgggctgc gcgaggcgcc gctggtcgag ctgatcggct tcgagcgcac
     2101 gcggctcgac gaggaggccg cgcaggcgct cggcagccgc cctggcgagc cgggactcgc
     2161 gatcgagacg cggctttccc tgcaggggcg ggcggtcgtc cacgaccgca tcgcgctccc
     2221 ggcggcgctg tttcgcgggc tgaccgagaa gcgcttccgc gaccggccga gcacgatcta
     2281 tcacctgtac cagatcgagt tcggcatcac cgtcgtgcgc gcgcacgagc ggctgcgcgc
     2341 cgccgccgct aaccgcgtgg cggcgcgctt gctcgggttg aaccacgggg cgccggtgtt
     2401 gcaggtgcgg cgtaccgcgc tcacgttcgg cgacaagccg gtcgaatacc gtgtgtcggc
     2461 cgtcaacacg gcacaccatg actatgtgaa tctgctgacg aggccgacat gagtggcatc
     2521 aaactcgcac gtgcgctcga ggtcgcgcag accgcggtcc gcgccgcagg gcgcttcctg
     2581 ctcgacagcc gcgcgcgcat cggaagcgcg gcgctcgagg gcagggcccc gcgcgaggtg
     2641 accgcgaaca tcgagcgcga ggccgaggcg ctgatccgca gccgcatcgg cgcggtgttt
     2701 ccggaacacg gattcgtcgg cgccgacctg ggcggcacgt tcgatgcgcg tcgcgcgcag
     2761 tggctggtcg acgcgctcga cggcacggac aactacctgc acggctatcc gcagtatgcg
     2821 gtgtcgctgg cgctgcagca ccagggcgag gccgtgctgg gcgtgatcta cgacccgcac
     2881 cgcgacgaac tcttcagcgc gacgcgcggg cagggcgcgt actgcaacgg ctcgcggctg
     2941 gcgtgcagca tgcgcgcggc gccggaagag gcgctggtcg cgacgatgtt tccgccgccg
     3001 aactcggagt gcatgccgcg attcctggag gaattcgcgc gcgtgaccaa tggctgcggc
     3061 ggcgtgcggc ggtccggcgc gctggcgctc gaccttgcgt atctggccgc gggccgcgtc
     3121 gacgcgttct gggcgcacga cgtcggtgcg tgggacgcgg cggccgcgat catcctgctg
     3181 cgcgaatccg gcgcgcgggt cgagtcgctc gacaacgggc cgctgctggc gtcgcggtca
     3241 ctgctcgcgg cggcgccgtc gctgctcgca cccacacgcg cgctgctggc cgggaggacg
     3301 tgatgtcgcc cacggccggt acaatcgcgc gatggccaag tcgacaccca agcccgtcgc
     3361 ggagttgagt ttcgagcagg cgctggacga actcgacgcg ctcgtgcggc gcatggaagc
     3421 gggcgagctg tcgctcga
//
LOCUS       DBNHBY010000254         2365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1368704, whole genome shotgun sequence.
ACCESSION   DBNHBY010000254 DBNHBY010000000
VERSION     DBNHBY010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1368704"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16HB_06235"
     CDS             <1..663
                     /locus_tag="AC16HB_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015734 - taurine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5606423.1"
                     /translation="ARNGSGINGLKDLKGKRIATPFVSTSHYQLMVALKLEGVDPKSV
                     NVMNLRPPEIAAAWERGDIDATFIWDPVLSKVKRNGKVIASSKSIGDKGYPTFDGYIV
                     NAKWAAENEAFMVAFVKLLAKADEEYRKNKAKWTADSPQVKAVAKWSKADAKDVPDAM
                     ALYSFPSLQEQASAKWLGGGAAKAMTDTAAFLKEQGRIQEVKPDYKAFVTDVYVKKAM
                     GK"
     gene            737..1525
                     /locus_tag="AC16HB_06240"
     CDS             737..1525
                     /locus_tag="AC16HB_06240"
                     /EC_number="7.5.2.-"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015734 - taurine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding protein"
                     /protein_id="HIR5606424.1"
                     /translation="MPTLELRELTVNYAVKGGTLQALAAVNLTMRDGDFVVALGASGC
                     GKTTLLNCIAGFLPASIGEILLDGRPVVGPGADRGVVFQKHALMPWLNVRDNVALGLR
                     MRGVDAATRARIAEDKLDLVGLRGFADRAVYELSGGMQQRVGIARALANDPAVLLMDE
                     PMGALDAFTREQLQEIILEAWSKSNKMVFFITHSVEEALFLATRLIVMSPSPGRITHV
                     YDDVPFSREFLTHHDARRVKSKPEFIRLREEVLALIHHREPAHA"
     gene            1518..>2365
                     /locus_tag="AC16HB_06245"
     CDS             1518..>2365
                     /locus_tag="AC16HB_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5606425.1"
                     /translation="MPEVISTPVVAQPADGAAPKTSKFKEPGEGSSAAISVATVLVLI
                     ALWFIVTNAGWIKPLFLPTPQTVALQFYDYLIGDVNDKPLWQHFVASILRVTIAFWAA
                     FATAVPVGVAMGMSRTARGIFDPPIEFYRPLPPLSYLPLIIIWFGIDELPKVLLIYLA
                     CFAPLALAARSGMKSASPEQIHAAYSMGATYWQVIRHVILPSALPEILVGMRIAIGFG
                     WTTLVAAEMVAASVGLGQMVLNASNFLRTDIVIMGIIVIGVVAYLFDLFMRWIEHKLV
                     PWKGRM"
BASE COUNT          403 a          766 c          802 g          394 t
ORIGIN      
        1 gcgcgcaacg gcagcggcat caacggcctg aaggacctga agggcaagcg catcgccacg
       61 ccgttcgtgt ccacgtcgca ctaccagctg atggtcgcgc tcaagctcga gggcgtggac
      121 ccgaagagcg tgaacgtgat gaacctgcgc ccgcccgaga ttgccgccgc gtgggaacgc
      181 ggcgacatcg acgcgacgtt catctgggac ccggtgctgt ccaaggtcaa gcgcaacggc
      241 aaggtgatcg cgagctccaa gtcgatcggc gacaagggct atcccacctt cgacggctac
      301 atcgtcaacg ccaagtgggc cgccgagaac gaggcgttca tggtggcgtt cgtcaagctg
      361 ctggccaagg ccgacgagga gtaccgcaag aacaaggcca agtggaccgc cgattcgcca
      421 caggtcaagg cagtggccaa gtggagcaag gccgacgcga aagatgtgcc ggatgcgatg
      481 gcgctgtatt cgttcccgag cctgcaggag caggccagcg cgaagtggct cggcggcggc
      541 gctgccaagg cgatgaccga cacggcggcg ttcctcaagg agcagggccg catccaggag
      601 gtcaagcccg actacaaagc cttcgtcacc gacgtgtacg tgaagaaggc gatgggcaag
      661 tagcgcactt tcattgcgcg gttcgaacgg ggcggcggcc actcgcgccg ccccgttctt
      721 ctatgcagga ctccgtatgc ccacgctcga actccgcgaa ctgacggtca attacgccgt
      781 caagggcggc acgctgcagg cgcttgcggc cgtgaacctg acgatgcgcg acggcgactt
      841 cgtggtcgcg ctcggcgcgt cgggctgcgg caagaccacg ctgctgaact gcatcgccgg
      901 cttcctgccg gcctccatcg gcgagatcct gctcgacggg aggccggtcg tcggtcccgg
      961 cgcggaccgc ggcgtggtgt tccagaagca cgcgctgatg ccgtggctga acgtcaggga
     1021 caacgtcgcg ctcggcctgc gcatgcgcgg cgtcgacgct gccacgcgcg cgcgtatcgc
     1081 cgaggacaag ctcgacctcg tcgggctgcg cggcttcgcc gaccgcgcgg tgtacgagtt
     1141 gtcgggaggc atgcagcagc gtgtcggcat cgcgcgcgcg ctggcgaacg acccggcggt
     1201 gctgctgatg gacgagccga tgggcgcact cgacgccttc acgcgcgagc aactgcagga
     1261 gatcattctc gaggcctggt cgaagtcgaa caagatggtg ttcttcatca cgcattcggt
     1321 cgaggaggct ttgttcctcg ccacgcgcct gatcgtgatg agtccgagcc caggccgcat
     1381 cacgcacgtc tacgacgacg tgccgttctc gcgcgaattc ctgacgcacc acgacgcgcg
     1441 cagggtgaag tcgaagccgg agttcatccg gctgcgcgag gaggtcctgg cgctgatcca
     1501 ccaccgggag cccgcccatg cctgaggtga tctccacgcc ggtcgtcgcg cagccggccg
     1561 acggcgccgc tccgaagacc agcaagttca aggagccggg agaaggctcc agcgcggcga
     1621 tcagcgtggc gaccgtgctc gtgctgatcg cgctgtggtt catcgtcacc aacgcgggct
     1681 ggatcaagcc gctgttcctg ccgacgccgc agacggtggc gctgcagttc tacgactacc
     1741 tgatcggcga cgtgaacgac aagccgctgt ggcagcactt cgtcgccagc atcctgcgcg
     1801 tgacgatcgc gttctgggcc gcgttcgcga ccgcggttcc ggtcggcgtc gcgatgggca
     1861 tgtcacgcac cgcgcgcggc atcttcgatc cgccgatcga gttctaccgg ccgctcccgc
     1921 cgttgtcgta cctgccgctg atcatcatct ggttcggcat cgatgagctg ccgaaagtgc
     1981 tgctgatcta tctcgcctgc ttcgcgccgc tggcgctggc cgcgcgctcg gggatgaaga
     2041 gcgcatcgcc cgagcagatc cacgccgcgt actcgatggg cgcgacctac tggcaggtga
     2101 tccggcacgt aatcctgccg tcggcgctgc ccgagatcct ggtcggcatg cggatcgcga
     2161 tcggcttcgg ctggaccacg ctggtggcgg ccgagatggt cgcggccagc gtcggcctcg
     2221 ggcagatggt gctcaacgca tcgaacttcc tgcgcaccga tatcgtgatc atgggcatca
     2281 tcgtgatcgg tgtggtggcg tacctgttcg atctcttcat gcgctggatc gagcacaagc
     2341 tcgtgccgtg gaagggccgg atgta
//
LOCUS       DBNHBY010000255         7675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_218587, whole genome shotgun sequence.
ACCESSION   DBNHBY010000255 DBNHBY010000000
VERSION     DBNHBY010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_218587"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="AC16HB_06250"
     CDS             complement(<1..421)
                     /locus_tag="AC16HB_06250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR5606426.1"
                     /translation="MDTHTVLFLVFAGILLAAALRVITARNPVHAVLFLVLSFFSAAC
                     LWMLLQAEFLAIVLVLVYVGAVMVLFLFVVMMLDINVDRLREGFWRHFPLAAVVGVAI
                     ALQLWLVLSHGGWQFLRTPGPGMPKGPNIEALGVLTFN"
     gene            complement(425..913)
                     /gene="nuoI"
                     /locus_tag="AC16HB_06255"
     CDS             complement(425..913)
                     /gene="nuoI"
                     /locus_tag="AC16HB_06255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR5606427.1"
                     /translation="MGAVAEFFNSLLLKELFKGLALTGRYLFSRKITIQFPEEKTPAS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRADGTRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETHVSEYHGEKRGDLYYTKDMLLAIGDRYEKEIAAAREADAR
                     YR"
     gene            complement(929..2017)
                     /gene="nuoH"
                     /locus_tag="AC16HB_06260"
     CDS             complement(929..2017)
                     /gene="nuoH"
                     /locus_tag="AC16HB_06260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR5606428.1"
                     /translation="MQGVTDFGVEAFGGAWPAVWALVKIVAIVVPLLVLVAYLTLWER
                     KLIGWMHIRLGPNRVGYGGLLQPWADAVKLIFKEVITPTQADKPLYFLAPMLVMMPAL
                     AAWAVIPFGPGVVLSDINAGLLFVMAVTSVGVYGVIIAGWASNSKYSFIGALRASAQM
                     VSYELAMGFVLVTVLLVAGTLNMTGIVNGQNTGIFADRGLNLLSWNWLPLLPLFVIYV
                     VSAVAETNRHPFDVVEGESEIVAGHMVEYSGMSFAVFFLAEYANMILLSTLATIMFLG
                     GWAAPVDLEKLLGLTFPGWVGAMSAWFWLFAKAFFVVSLFIWFRASFPRYRYDQIMRL
                     GWKVFIPITLVWLVVIAVWMQTPWNIWK"
     gene            complement(2017..4332)
                     /gene="nuoG"
                     /locus_tag="AC16HB_06265"
     CDS             complement(2017..4332)
                     /gene="nuoG"
                     /locus_tag="AC16HB_06265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR5606429.1"
                     /translation="MVELEIDGRKVSVPEGSMVMDAANQLDIYVPHFCYHKRLSIAAN
                     CRMCLVEVEKAPKPLPACATPVTQGMIVRTASARAKQAQRGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSASRYVEPKRVVFHKDLGPLISAEEMTRCIHCTRCVRFGQEI
                     AGIMELGMAGRGEHSEILSFVGETVDSEISGNVIDLCPVGALTSKPFRYAARTWELSR
                     RRSVSPHDALGSNLIVQVKNYRVLRVVPLENEAINDCWLSDRDRFSYEGLYAADRLTQ
                     PMIRQASGWREVDWSDALTHVATLLNAAKVKHGAAAIGALAGAGSTAEELFLLGKLVR
                     ALGSDNVDFRLRQSDFSADGQRGGVPWLGMPVADLDNLDRLLLVGSFLRKDHPLLAVR
                     VRTAVKRGCKASVMHAVDDDLLMPVAHKVIVRPSAWVDELAQIAAAVAQIKGVAAPVA
                     TEPGAAARAIAESLCSGHNAAVLLGNAAVQSPHAVQLQAWSQWIAQATGARFGVLGEA
                     ANSVGGYLVGALPRTGGLNARAMVEQPRRAYLLWNMEPEFDVADPSATHAALAAADAV
                     IAFSPYRNGALEYAHAILPITPFTETSGTFINIEGRAQTFNGSVRPLGDSRPGWKVLR
                     VLGNALELPGFDYDTPEAVRAEAIPPDIPARLSNKIDVAPARIAPATNPERVADVPPY
                     FTDAIVRRAESLQATRDARPPKATANANTLASFGLAAGAKARVRQGEASALLECALDD
                     RLPDGVVRVPAGHASTATLAALFGPITLERA"
     gene            complement(4336..5640)
                     /gene="nuoF"
                     /locus_tag="AC16HB_06270"
     CDS             complement(4336..5640)
                     /gene="nuoF"
                     /locus_tag="AC16HB_06270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR5606430.1"
                     /translation="MTLQTCLHGRHIDPILLAGLNGDNWRLRDYEARGGYAALKKILA
                     EKIAPDAVIAEVKKSALRGRGGAGFPTGLKWSFMPRQFPGQKYLVCNSDEGEPGTFKD
                     RDILRYNPHIVIEGMAIAAYAMGTSVGYNYIHGEIWADYERFQQALDEAYAAGYLGQR
                     ILGSAFSFDLYAAHGYGAYICGEETALLESLEGKKGQPRFKPPFPASYGLYGKPTTIN
                     NTETFAAVPWIINHGGDAFLNIGRPNNGGTKIFSVSGDVEKPGNYEVPLGTPFAKLLE
                     LAGGMRGGRKLKACIPGGSSMPVLPGDVMMACDMDYDSIAKAGSMLGSGAVIIMDETR
                     CMVRCLERLSYFYFEESCGQCTPCREGTGWLYRVVHRIETGQGRPEDLDLLNSVADNI
                     QGRTICALGDAAAMPVRAFIKHFRSEFEHHIEHRHCLVPEYV"
     gene            complement(5637..6137)
                     /gene="nuoE"
                     /locus_tag="AC16HB_06275"
     CDS             complement(5637..6137)
                     /gene="nuoE"
                     /locus_tag="AC16HB_06275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5606431.1"
                     /translation="MLSATALAQIDRELAKYPPEHRQSAVMAALAIAQDEHGWISKEL
                     MQEVADYLGMPAIAVYEVANFYTMYNTAPVGKYKITVCTNLPCALSGANRAVNHLKQR
                     LGIEFNETTADGHFTLKEGECMGACGDAPVLLVNNKRMCSFMSDERLDALLAELNREL
                     RPGKDR"
     gene            complement(6161..7414)
                     /locus_tag="AC16HB_06280"
     CDS             complement(6161..7414)
                     /locus_tag="AC16HB_06280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5606432.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETRTFIQSVPYMDRLDYVSMMCNEHAYVMAIEKLIGVEVPVRAQFIRVMFD
                     EITRILNHLLWIGAHALDVGAMAVLLYAFREREDLFDVYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYKPSRYRDATELKRLNENRQGSVLDFIEDFTRRFPTYVDEYETLLTDNR
                     IWKQRLVGIGVVSPERALQMGFTGPMLRGSGIEWDLRKKQPYEVYDRVAFDIPVGRNG
                     DSYDRYLVRVEELRQANRIIQQCVDWLRANPGPVMTDDHKVAPPSRIDMKSNMEELIH
                     HFKLFTEGFHVAAGETYAAVEHPKGEFGIYLVSDGANKPYRMKIRAPGFAHLASLDEM
                     AKGHMIADAVTIIGTQDIVFGEIDR"
     gene            complement(7407..>7675)
                     /locus_tag="AC16HB_06285"
     CDS             complement(7407..>7675)
                     /locus_tag="AC16HB_06285"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="complex I 30 kDa subunit family protein"
                     /protein_id="HIR5606433.1"
                     /translation="TSVWNCANWYEREAFDLFGIMFEGHPDLRRILTDYGFVGHPFRK
                     DFPVSGHVEMRYDPELKRVIYQPVSIEPREVTPRVIREENYGRG"
BASE COUNT         1320 a         2636 c         2443 g         1276 t
ORIGIN      
        1 cgttgaaggt cagcacgccg agcgcctcga tgttcggccc cttgggcatg ccgggcccgg
       61 gtgtgcgcag gaactgccag ccgccgtgcg acagcaccag ccacagttgc agcgcgatcg
      121 ccacgccaac cacggcggcc agcgggaaat ggcgccagaa gccctcgcgc aggcggtcga
      181 cgttgatgtc cagcatcatc accacgaaca ggaacagcac catcaccgcg ccgacgtaaa
      241 ccagcaccaa cacgatcgcg aggaactccg cctgcagcag catccacagg caagcggcag
      301 agaagaacga aagcaccagg aaaagcaccg catgcaccgg attgcgcgca gtgatgacgc
      361 gcagcgccgc ggcgagcagg atgccagcga agaccaggaa caggacggta tgggtatcca
      421 tgcgttatcg gtagcgggca tccgcctcgc gcgcggcggc gatttccttc tcgtagcgat
      481 cgccgatcgc cagcagcatg tccttcgtgt agtacaggtc gccgcgcttc tcgccgtggt
      541 actcggagac gtgcgtctcg acgatcgagt cgaccgggca gctctcctcg cagaagccgc
      601 agaagatgca cttggtcagg tcgatgtcgt agcgcgtcgt gcggcgcgtg ccgtcggcgc
      661 gctgctcgct ctcgatcgtg atcgccagcg ccggacacac cgcttcgcac agcttgcacg
      721 cgatgcagcg ctcctcgccg ttcggatagc ggcgcagcgc gtgcaggccg cggaagcgtg
      781 gcgacgccgg cgtcttctcc tctggaaact ggatcgtgat cttgcgcgaa aagaggtacc
      841 gtccggtcag ggccagcccc ttgaacaact ccttcagcag cagcgaattg aagaactcgg
      901 cgacggcgcc catggcccta ttcccggtct acttccagat gttccacggc gtctgcatcc
      961 acactgcgat cacgaccagc cacaccagcg tgatcgggat gaacaccttc cagccaagcc
     1021 gcatgatctg gtcatagcgg tagcgcggaa acgacgcgcg gaaccagatg aacagcgaca
     1081 cgacgaagaa cgccttggcg aagagccaga accaggcgct catcgcaccg acccacccgg
     1141 ggaaggtcag gccgagcagc ttttccaggt ccaccggcgc ggcccagccg ccgaggaaca
     1201 tgatcgtcgc cagcgtcgac agcaggatca tgttcgcgta ttcggcgagg aagaagacgg
     1261 cgaagctcat gccggagtac tcgaccatgt gcccggcgac gatctcgctc tcgccctcga
     1321 ccacgtcgaa aggatggcgg ttggtttcgg cgaccgccga caccacgtag atcacgaaca
     1381 gcggcagcag cggcagccag ttccacgaca ggaggttgag gccgcggtcg gcgaagattc
     1441 cggtgttctg cccgttgacg atgccggtca tgttcagcgt cccggccacc agcagaaccg
     1501 tcaccagcac gaagcccatc gccagctcgt agctcaccat ctgcgccgaa gcgcgcagcg
     1561 cgccgatgaa cgagtacttg gagttgctcg cccagcccgc gatgatcacg ccatacacgc
     1621 cgaccgaggt gaccgccatc acgaacagca accccgcgtt gatgtccgac agcaccacgc
     1681 cggggccgaa cggaatcacc gcccacgccg ccagcgccgg catcatcacc agcatcggcg
     1741 ccaggaagta caacggcttg tcggcctgcg tcggtgtgat gacttccttg aagatcagct
     1801 tgaccgcgtc ggcccacggc tgcagcagcc cgccatagcc gacgcggttc gggccgagcc
     1861 ggatgtgcat ccagccgatc agcttgcgtt cccacagcgt gagatacgca accagcacca
     1921 gcagcgggac gacgatcgcg acaatcttca ccagcgccca cactgccggc caggcgccgc
     1981 cgaatgcctc cacgccgaag tccgtcacgc cctgcatcac gcgcgctcca gcgtgatcgg
     2041 tccgaacagc gcggcaagcg tggccgtcga cgcgtggccc gcaggcacgc gcaccacgcc
     2101 gtccggcagg cgatcatcga gtgcgcattc gagcagtgcg ctcgcctcgc cctgccggac
     2161 gcgcgccttg gcgcccgcag cgagcccgaa gctcgccagc gtattcgcgt tcgcggtcgc
     2221 cttcggcggc cgcgcatcgc gcgtggcctg cagcgattcg gcgcggcgca cgatcgcgtc
     2281 ggtgaagtac ggcggcacat cggccacacg ctcggggttg gtggcgggtg cgatgcgcgc
     2341 gggcgcgacg tcgatcttgt tcgacaagcg cgcggggatg tcgggcggaa tcgcctcggc
     2401 gcgtacggct tccggcgtgt cgtagtcgaa gcccggaagc tccagtgcgt tgccgagcac
     2461 gcgcagtact ttccagcccg gccgcgagtc gccgagcggc ctcacgctgc cattgaaggt
     2521 ctgcgcgcgg ccttcgatgt tgatgaaagt ccccgacgtt tcggtgaatg gcgtgatcgg
     2581 caggatcgcg tgtgcatact ccagcgcgcc gttgcgatac ggcgaaaacg cgatcaccgc
     2641 gtccgccgct gcaagcgccg cgtgcgtcgc ggacggatcg gcaacgtcga actcgggctc
     2701 catgttccac agcaggtacg cgcgccgcgg ctgttcgacc atcgcacgcg cattcaggcc
     2761 gccggtccgg ggcagtgctc cgacgaggta accgccgacg ctgttggctg cctcaccgag
     2821 cacgccgaaa cgcgcaccgg tcgcctgcgc gatccactgg gaccaagcct gcagttgcac
     2881 cgcgtgcggc gattgcacgg cggcattgcc aagcagcact gccgcgttgt gtccgctgca
     2941 caggctctcg gcgatcgcgc gggccgccgc gcctggctcc gtggcgaccg gtgcggcaac
     3001 gcccttgatc tgcgcgaccg ccgcggcgat ctgcgccagt tcgtccaccc acgccgacgg
     3061 ccgcacgatc accttgtgcg caactggcat cagcaggtcg tcgtcgaccg catgcatgac
     3121 gctcgccttg cagccgcgct tgacggcggt gcgcacgcgc accgcgagca gtggatggtc
     3181 cttgcgcagg aacgagccga ccagcagcag ccggtcgagg ttgtcgaggt ccgccacggg
     3241 catgccgagc cacggcacgc cgccgcgctg gccatcggcg gaaaagtcgg attgccgcag
     3301 ccggaagtcg acgttgtcgc tgcccaacgc gcgcaccagt ttgcccagca ggaacagttc
     3361 ctcggcggta ctgccggcgc cggccagcgc gccgatcgcg gcggcgccat gcttcacctt
     3421 cgcggcgttc agcagcgtcg ccacgtgcgt cagcgcatcg gaccaatcga cctcgcgcca
     3481 cccgctcgcc tgccgaatca tcggctgggt caggcgatcg gctgcgtaca ggccctcgta
     3541 ggagaagcgg tcgcggtcag agagccagca gtcgttgatc gcctcgttct ccagcggcac
     3601 cacgcgcagc acgcggtagt tctttacctg cacgatcaga ttggagccga gcgcatcgtg
     3661 cgggctcacg ctcctgcggc gcgacagctc ccacgtgcgc gccgcgtaac gaaacggctt
     3721 gctcgtcagt gcgccgaccg ggcacaggtc gatcacgttg cccgatatct cggagtcgac
     3781 cgtctcgcca acgaacgaaa ggatctcaga gtgctcgccg cggccggcca tgccgagttc
     3841 catgatgccg gcgatctcct gcccgaaccg cacgcagcgc gtgcagtgga tgcagcgggt
     3901 catttcctcc gccgagatca gcgggccgag atccttgtgg aacacgacgc gcttgggctc
     3961 cacgtagcgc gatgccgaac cgccgtagcc gaccgcgaga tcctgcagct ggcactcgcc
     4021 gccctggtcg cagatcgggc agtcgagcgg atggttgatc agcaggaact ccatcacgcc
     4081 gcgctgcgcc tgcttggcgc gcgcgctcgc ggtgcgcacg atcatgccct gcgtgaccgg
     4141 cgtggcgcac gccggcagcg gcttcggcgc cttctcgacc tcgaccaggc acatgcggca
     4201 gttggccgcg atcgacagcc gcttgtggta acagaagtgc ggcacgtaga tgtcgagctg
     4261 gttggccgca tccatcacca tgctgccctc gggcacggac accttcctgc cgtcgatttc
     4321 cagttcgacc atgacctaga cgtactccgg caccaggcag tgcctgtgct cgatgtggtg
     4381 ctcgaactcc gagcggaagt gcttgatgaa cgcgcgcacc ggcatcgcgg ccgcgtcgcc
     4441 gagcgcgcag atggtgcggc cctggatgtt gtcggccacc gagttcagca ggtcgaggtc
     4501 ctcgggccgg ccctggccgg tctcgatccg gtgcaccacg cgatacagcc agccggtgcc
     4561 ctcgcggcac ggcgtgcact ggccgcagct ctcctcgaaa tagaagtacg acagtcgctc
     4621 caggcagcgc accatgcagc gtgtctcgtc catgatgatc accgcgccgg agccgagcat
     4681 ggaaccggcc ttggcgatcg agtcgtagtc catgtcgcac gccatcatca cgtcgcccgg
     4741 cagcaccggc atggacgaac cacccgggat gcaggccttc agcttgcgcc cgccgcgcat
     4801 gccgccagca agttcgagca gcttcgcgaa cggcgtgccg agcgggactt cgtagttgcc
     4861 gggcttctcg acgtcgcccg acaccgagaa gatcttggtg ccgccgttgt tcggccggcc
     4921 gatgttcagg aacgcgtcgc cgccgtgatt gatgatccaa ggcaccgcgg cgaaggtttc
     4981 ggtgttgttg atcgtggtcg gcttgccgta caggccgtag ctcgcgggga aaggtggctt
     5041 gaagcgcggc tggcccttct tgccctcgag cgactcgagc agcgcggttt cctcgccgca
     5101 gatgtacgcg ccgtagccat gtgcggcgta caggtcgaac gaaaacgccg atccgaggat
     5161 cctctggccg agatagccgg ccgcgtacgc ctcgtcgagc gcctgctgga agcgttcgta
     5221 gtcggcccag atctcgccgt ggatgtagtt gtagccgacg gaagtaccca tcgcgtaggc
     5281 cgcgatcgcc atgccctcga tgacgatgtg cgggttgtag cgcaggatgt cgcggtcctt
     5341 gaatgtgccc ggctcgcctt cgtccgagtt gcagacgagg tacttctgcc ccggaaactg
     5401 gcgcggcatg aagctccact tcaatcccgt cggaaagccc gcgccgccgc ggccgcgcag
     5461 cgccgacttc ttcacctcgg cgatcaccgc gtcgggcgcg atcttctcgg ccaggatctt
     5521 cttcagcgcc gcgtagcccc cgcgcgcctc gtagtcgcgc aggcgccagt tgtcgccgtt
     5581 caacccggcg aggaggatcg gatcgatgtg gcggccatgc aagcaggtct gcagcgtcat
     5641 cggtccttgc ccggccgcaa ttcgcggttc agttcggcca gcagcgcgtc gagcctttcg
     5701 tcgctcatga agctgcacat ccgcttgttg ttcaccagca gcaccggcgc atcgccacac
     5761 gctcccatgc actcgccttc cttcagcgtg aagtgcccgt cggcggtcgt ctcgttgaac
     5821 tcgatgccga gccgctgctt caggtggttc acggcacggt tcgcgcccga cagcgcacac
     5881 ggcagattgg tgcagacggt gatcttgtac ttaccgaccg gcgcggtgtt gtacatcgtg
     5941 tagaagttcg cgacctcata caccgcgatc gccggcatgc cgaggtagtc ggcgacttcc
     6001 tgcatcagct ctttcgagat ccagccgtgc tcgtcctgcg cgatcgccag cgccgccatc
     6061 actgccgact gccggtgctc cggcgggtac ttggccagct cgcgatcgat ctgcgccagc
     6121 gccgtcgcgg acagcagcgt tcgcggccgc ggtacggcgc tcatcgatcg atctccccga
     6181 acacgatgtc ctgcgtgccg atgatggtga ccgcgtcggc gatcatgtgg cccttcgcca
     6241 tctcgtccag cgacgcgagg tgcgcgaatc ccggcgcgcg gatcttcatc cggtacggct
     6301 tgttggcgcc gtcgctgaca agatagatgc cgaactcacc ctttggatgc tcgaccgcag
     6361 cgtaagtctc gccggccgcc acatggaaac cctcggtgaa gagcttgaag tggtggatca
     6421 actcctccat gttcgatttc atgtcgatcc gcgacggcgg cgcgaccttg tggtcgtcag
     6481 tcatcaccgg gcccggattc gctcgcagcc agtcgacgca ctgctggatg atgcgattgg
     6541 cctgccgcag ctcctcgacg cgcaccagat agcggtcgta cgagtcgcca ttgcggccga
     6601 ccgggatgtc gaacgccacg cggtcgtaga cctcgtaagg ctgcttcttg cgcaggtccc
     6661 attcgatgcc ggagccgcgc agcatcggcc cggtgaagcc catctgcagc gcgcgctcgg
     6721 gcgagaccac gccgatgccg accagccgct gcttccagat ccggttgtcg gtcagcagcg
     6781 tctcgtactc gtccacgtag gtcgggaaac gccgcgtgaa atcctcgatg aagtcgagca
     6841 ccgagccctg gcgattctcg ttcagccgct tgagttccgt tgcgtcgcgg tacctgctcg
     6901 gcttgtactg cggcatcgag tccggcaggt cgcgatacac accgcccggc cggtagtagg
     6961 cagcgtgcat gcgcgcgccg gacaccgcct cgtagacgtc gaacaggtcc tcgcgctcgc
     7021 ggaacgcata cagcagcacc gccatcgcgc ccacgtcgag cgcgtgcgcg ccgatccaca
     7081 gcaggtggtt caggatgcgc gtgatctcgt cgaacatcac gcgaatgaac tgcgcgcgga
     7141 cgggcacctc gacgccgatc agcttctcga tcgccatcac gtacgcgtgc tcgttgcaca
     7201 tcatcgacac gtaatcgagc cggtccatgt acggcaccga ctggatgaac gtgcgcgtct
     7261 cggccagctt ctccgttgcc cggtgcagca gtccgatgtg cggatccgcg cgctggatca
     7321 cctcgccgtc gagctcgagc accagccgca gaacaccatg cgccgcgggg tgctggggac
     7381 caaagttcaa cgtgtagttc ttgatctcag ccacgcccgt agttctcctc gcgaatcacc
     7441 cgcggcgtga cttcgcgcgg ttcgatcgac accggctggt agatcacgcg cttcagctcg
     7501 gggtcgtagc gcatctcgac gtggcccgac accgggaaat ccttgcggaa cggatggccc
     7561 acgaacccgt agtcggtgag gatgcggcgc aggtccgggt ggccctcgaa catgatgccg
     7621 aagaggtcga atgcctcgcg ctcataccag ttcgcacagt tccagacgct ggtca
//
LOCUS       DBNHBY010000256         2939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1165594, whole genome shotgun sequence.
ACCESSION   DBNHBY010000256 DBNHBY010000000
VERSION     DBNHBY010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1165594"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1059
                     /locus_tag="AC16HB_06290"
     CDS             58..1059
                     /locus_tag="AC16HB_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HIR5606434.1"
                     /translation="MSHALTAAIAARLARVALDNVGREYPYHVLHLLNGDADARPPRE
                     LHPVFHGSYDWHSCVHMHWTLARCLRRFPVLPEAASIAAHFDARFTPQAVGREIAYLE
                     APGRTTFERPYGWGWLLALHAELARLAHKSWHTALAPLAALIAQRIVDWLPRQDYPVR
                     AGTHANTAFALLHALDHAQRERHPALRAAIVGRTHAWFAADRDYPAGYEPGGDDFLSG
                     GLCEALLMLRATDDSTFADWWQAFEAAPAALARWLAPVAVSDPHDAKIVHLHGLNLSR
                     AWCWRGLAPHLPKPLQPAVAAAIESHLDASVQSALAGDYVGTHWLASFALLALDAPD"
     gene            1116..2687
                     /locus_tag="AC16HB_06295"
     CDS             1116..2687
                     /locus_tag="AC16HB_06295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5606435.1"
                     /translation="MESHPAPADAIVIGSGIGGLAAAAALATQGRRVLVLERHTHAGG
                     LTQAFERDGFRFNIGVHYLGGFGPGSLNRRIFDLLGGGRIALAPIPGTYDRIRFPGFE
                     TAFSPPREALVQTLKAAFPEEGAGIDRYFDAIDHGVRALGAAFVTHSAPPLVAKTMQW
                     LRGAEIERWVGSTTWDTVCDCVRDPRLRAVLCAQWGDYGSRPQESSFAMHATVAQHYF
                     DGAWYPVGGSGSFAREMGATIAAAGGAIRTGAEVAAIRMHEGRVAGVTLAGGETLDAP
                     CVISDAGMRNTLRMLPSQEIDYAWASDVLELEPSFGHVGLYLGIEGDIAALGADTANE
                     WIYESWDVNALWRDPFTQQRAPALFVSFPSLRDPAHDPGPRQRHTAEVVALVDWNVFA
                     QWDRSLEPGGMRAGTAAAERSESYLAFKALLEQNLLAQFAHHFPDVAARVVQHTASTP
                     ISVATFIGSEHGAIYGLETSPRRFLSNALKPRTPVGGLFLAGQDASTPGIAGAMMGGL
                     MAAASVEPKLWSMLR"
     gene            complement(2742..>2939)
                     /locus_tag="AC16HB_06300"
     CDS             complement(2742..>2939)
                     /locus_tag="AC16HB_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5606436.1"
                     /translation="STFFMLTGFHGFHVIVGAIMLTVMFFRILRGHFRPDHHFAFEAA
                     AWYWHFVDVVWIGLYIVVYWL"
BASE COUNT          435 a         1013 c         1036 g          455 t
ORIGIN      
        1 gggcgacgag agcgccgact cgctgcgcta gttaagcctg cctgctaacg tgcaggcgtg
       61 tcccatgcat tgactgcagc gattgccgcc cggctcgccc gcgttgcgct cgacaacgtc
      121 ggccgcgagt atccgtacca cgtgcttcac ctgctgaatg gcgacgccga cgcgaggccg
      181 ccgcgtgagc tgcatccggt gttccacggc agctacgact ggcattcgtg cgtgcacatg
      241 cactggacgc tggcgcgctg cctgcggcgc ttccccgtcc tgcccgaggc ggcttcgatc
      301 gccgcgcact tcgatgcgcg cttcacgccg caggccgtgg gacgcgagat cgcgtacctc
      361 gaggcaccgg gccgaacgac cttcgagcga ccgtacggct ggggctggct gctcgcgctg
      421 cacgccgagc tcgcacggct cgcgcacaag tcctggcaca cggcgctggc gccgctcgcg
      481 gcgctgattg cgcagcgcat cgtcgactgg ctgccgcggc aggactaccc ggtgcgcgcc
      541 ggcacgcatg ccaacaccgc gttcgcgctg ctccatgcgc tcgaccatgc gcaacgcgag
      601 cggcatccgg cgctgcgcgc ggcgatcgtc gggcgcacgc acgcctggtt cgcggccgat
      661 cgtgactacc cggccgggta cgagcccggc ggcgacgatt tcctctccgg cggcctgtgc
      721 gaggcgctgc tgatgctacg cgcgaccgac gactccacat tcgccgactg gtggcaggcg
      781 ttcgaagccg cccccgcggc actggcgcgc tggctggccc cggtcgcggt cagcgacccg
      841 cacgacgcca agatcgtgca tttgcacggg ctgaacctgt cgcgcgcctg gtgctggcgc
      901 gggctggcgc cgcacctgcc caagccgctg cagccggccg tcgccgctgc gatcgagtcg
      961 cacctcgacg catcggtcca gtccgcgctc gccggcgatt acgtcggaac gcactggctc
     1021 gcgtcattcg cgctgctcgc cctcgacgcc cccgattgac cgtcatcaac gcgccattcc
     1081 cgcgcgctgc tgagattgcg ccagggaggt cgggaatgga aagccatcca gcgcctgcgg
     1141 acgcgatcgt catcggctcg ggcattggcg gattggctgc ggcggcggcg ctggccacgc
     1201 aaggtcgccg ggtgctggtg ctcgagcggc acacgcacgc cggcggactg acgcaggcat
     1261 tcgagcgcga cggctttcgc ttcaacatcg gcgtgcacta tctcggcgga ttcgggcctg
     1321 gcagcctgaa tcggcgcatc ttcgatctgc tcggcggcgg gcgcatcgcg ctggcgccga
     1381 tccccggcac gtacgaccgt attcggttcc cgggcttcga gaccgcgttc tcgccgccgc
     1441 gcgaagcgct ggtgcagacg ctgaaggcgg catttccgga ggaaggcgcc ggcatcgatc
     1501 gctacttcga cgcgatcgat cacggcgtgc gcgcactcgg cgcggcgttc gtcacgcaca
     1561 gcgcgccgcc gctggtcgcc aagacgatgc agtggttgcg cggcgccgag atcgagcggt
     1621 gggtcggcag cacgacgtgg gacacggtat gcgattgcgt gcgcgatccg cgcctgcgcg
     1681 cggtgttgtg cgcgcaatgg ggcgattacg gcagccggcc acaggagagc tcgttcgcga
     1741 tgcacgcgac cgtcgcgcag cactacttcg acggcgcgtg gtatccggtc ggcggctcgg
     1801 ggagcttcgc gcgcgagatg ggtgccacga tcgcggccgc gggcggcgcg atccgcaccg
     1861 gcgccgaggt cgcggcgatc cgcatgcacg aagggcgcgt cgcgggcgtg acgcttgccg
     1921 gcggcgaaac actggatgcg ccgtgcgtga tctccgatgc cggcatgcgc aacacgctgc
     1981 ggatgctgcc gtcgcaggag atcgactacg cgtgggcgag cgacgtgctg gagctcgagc
     2041 cgtcgttcgg ccacgtgggg ctgtacctcg gcatcgaggg cgatatcgcc gcgctcggcg
     2101 ccgacaccgc caatgaatgg atctacgaga gctgggacgt caacgcgctg tggcgcgacc
     2161 cgttcacgca gcagcgcgcg ccggcgctgt tcgtatcgtt cccgtcgctg cgcgatcccg
     2221 cgcacgatcc ggggccgcgc cagcgacaca ccgcggaggt cgtggcgctg gtcgactgga
     2281 acgtgttcgc gcagtgggac cgctcgctcg agcccggcgg aatgagggcc ggcacggccg
     2341 cggccgagcg cagcgaaagc tatctcgcgt tcaaggcgct gctggagcag aacctgctcg
     2401 cgcagttcgc gcaccacttt cccgacgttg ccgcgcgcgt cgtgcagcac accgcatcga
     2461 cgccgatttc ggtggcgacc ttcatcggtt ccgagcatgg cgcgatctac gggctggaga
     2521 catcgccgcg ccggttcctg tcgaacgcat tgaagccccg tacgccagtc ggcggactgt
     2581 tccttgccgg ccaggatgcg tctacgcctg gcatcgccgg cgcgatgatg ggcgggctga
     2641 tggccgccgc cagcgtcgag ccgaagttgt ggtcgatgct gcgttgagac ttcagaaacg
     2701 cagacggcgc ccacggcgcc gtctgcgtgg gccgccgagg ctcacagcca gtacacgacg
     2761 atgtacaggc cgatccagac cacgtcgacg aagtgccagt accaggcggc ggcctcgaac
     2821 gcaaagtgat ggtcgggacg gaagtggccc ctgaggatgc ggaagaacat cacggtcagc
     2881 atgatcgcgc cgacgatcac gtggaaaccg tggaatccgg tcagcatgaa gaacgtcga
//
LOCUS       DBNHBY010000257         5843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_623959, whole genome shotgun sequence.
ACCESSION   DBNHBY010000257 DBNHBY010000000
VERSION     DBNHBY010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_623959"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1038
                     /gene="selD"
                     /locus_tag="AC16HB_06305"
     CDS             16..1038
                     /gene="selD"
                     /locus_tag="AC16HB_06305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:43..45,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR5606437.1"
                     /translation="MTEMVACAGUASKLAAGELAQVLGDIPLNPDPRVLVDFRHSDDA
                     GVYAWEGGPALVQTVDFFTPIVDDPYMYGQIAAANSLSDVYAMGGRPLTALAIAGFPK
                     GTLTKEVVGAVFRGGYDKLREAGVALLGGHTVQDPEIKFGYAVTGAIDPARILHNAGA
                     KEGDRVLLTKPLGTGVVGTAIKNGRAPEALVAQAIESMRTLNRAAAEVIDRLPGVNSC
                     TDITGFGLIGHASEVANASGVTLVLDAAQVPLFDGVKALVRRNRSAGMKSNREHFGSG
                     VAVGAGIDDDLLDLCYDPQTSGGLLIFVAEAHAPAALAALRAANVPAVEIGRAVAPTG
                     HWIELR"
     gene            1041..1505
                     /gene="nikR"
                     /locus_tag="AC16HB_06310"
     CDS             1041..1505
                     /gene="nikR"
                     /locus_tag="AC16HB_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HIR5606438.1"
                     /translation="MERFTISLSEELAKEFDALIATRGYENRSEAVRDLIRRELESLR
                     RTRDPHAHCVANLSYVYNHHERDLADRLIELQHEYHDLCVSTLHAHLDHDHCMESLIL
                     RGHLDEVQSFADRLVAERGVRHGQLNVVTVEPGRAHRHGHGAPPHTHFRPHD"
     gene            complement(1512..3035)
                     /locus_tag="AC16HB_06315"
     CDS             complement(1512..3035)
                     /locus_tag="AC16HB_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5606439.1"
                     /translation="MQIRLLDELGLDPRARVMLLLTLVFTTLVIRAVTLGTYALTDTT
                     EARYGEIARVMLATGNWITPQEMPGQPFWAKPPLYAWLSAASMALLGVNELAARLPSF
                     LFAVGTLALVFSWARDGGSNGTPWLALERGLMAAAILATSVLFYASAGAVMTDPSLAF
                     CSTWMMVAYMQTLLRGQTGWFWRWGFFIAAGLGMLAKGPVVMVYAGLPIALWTLATGR
                     VRAAWRALPWLRGTLLFAALWVPWYAAAEMRTPGFLSYFLLGEHVMRFIDPGWRGDLY
                     GAAHVRPRGFIWLFLLGALGVWAPVVIALLVSRVRASGLRVPLRDADPQFTYALLCAA
                     VPLLFFTFARNIIWPYVLPMLSPLAVLLANAFERRLPRLAWARAFMSGLPLAWISLAA
                     AWIAWAPYESRDRSTESLVELWRQEGIAEPGPLLYWGDRVPHSLRFYSRDKATAFIGT
                     ATTPAPPPPVFMIVERRRISEVEQWLAATHPGRDFEPLAGNARWVLAEIELPRPPPH"
     gene            3171..3995
                     /locus_tag="AC16HB_06320"
     CDS             3171..3995
                     /locus_tag="AC16HB_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HIR5606440.1"
                     /translation="MHNLPTEWTALAALVFLLGLKHGFDADHLATIDGLTRINARARR
                     SWARYCGALFSLGHGAVVLAIALGVGLASAKWDAPQWLEASGAWISIAFLAALGILNL
                     HAVLATPRHQIVAPVGLKGRLLGRLNRAQHPALVALVGALFALSFDTVSQAALFAMTA
                     AQFGGVTHALMLGLLFMLGMMVTDGINGLWISRLIARADQLALIASRVMSLAVSGVSL
                     LVAALGAAKLAVPVVDTWSDGKELAFGAVVVAVVAGSYLAARALALQPGAAADRRT"
     gene            complement(4001..4507)
                     /locus_tag="AC16HB_06325"
     CDS             complement(4001..4507)
                     /locus_tag="AC16HB_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5606441.1"
                     /translation="MNSRRTWISAAVAAVLIGAAVPALSQGPGYGPGPGAHRSAPMAG
                     AQPERAQARIEALRNELKLAPNQVAAFDAWAAKVRSEAEARAKLRTEMQNRMGDAQAM
                     SEFHATMAKHHAEAAQELHTLRTNLYAVLTAEQKKVLDSYGPGARMGQRGAGWGPGNG
                     PRRGCMST"
     gene            4718..>5843
                     /locus_tag="AC16HB_06330"
     CDS             4718..>5843
                     /locus_tag="AC16HB_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5606442.1"
                     /translation="MKNWFFRCVPAVLVSLLVACGGSNGGSASPPTNVTTQSGDGWIR
                     VNWDSAGGLEYWVFTALDASLTTLNWLNLVGGAAFINVTPPLTLCSQTNGLQRWFTVN
                     SRSGSDPGGPGSAVVSAVPSAAGANWTGGATLGDDLAGIGFGVLTTCLRTGLPTGVLT
                     AVGPGAVIYSSSDAGANWTRRAAPSGFTGDLNAVANYTANPNSTTASPGMRTVAVGAG
                     GASIYSTDSSVTWTVGRAPSASSATLRGLVTVSSAFVAVGDGGTIQSSSDGITWNALP
                     SNTTTNLRGLAFGNSRYVAVGDGGTLVTSTDSGVSWTVQTIAGAGNLRAVAYGSNNNN
                     TDNGGTLLINTFVAVADNGSAVVSHEGGVNWSVVAVPGAGE"
BASE COUNT          917 a         2025 c         2040 g          861 t
ORIGIN      
        1 ttcggcaaga agcgcctgac cgaaatggtc gcctgcgcgg gttgagcgag caagctcgcc
       61 gccggcgagc tcgcccaggt cctgggcgac attccgctca accccgatcc gcgcgtcctc
      121 gtcgatttcc ggcactcgga cgacgcgggc gtttacgcct gggagggcgg gcctgcgctc
      181 gtgcagacgg tggatttctt cacgccgatc gtcgacgacc cgtacatgta cgggcagatc
      241 gcggcggcca attcgctcag cgacgtgtac gcgatgggcg gccgtccgct gactgcgctg
      301 gccatcgccg gctttccgaa gggcacgctg acgaaggaag ttgtcggcgc ggtgttccgc
      361 ggcggctacg acaagctgcg cgaggccggc gtggcgctgc tcggcggtca cacggtgcag
      421 gaccccgaga tcaagttcgg ttacgcggtc accggcgcga tcgacccggc gcgcatcctg
      481 cacaacgcgg gcgcgaaaga aggcgaccgc gtgctgctga ccaagccgct cggcaccggc
      541 gtggtcggca ccgcgatcaa gaatggccgc gcacccgagg cgctcgtggc gcaggcgatc
      601 gaatcgatgc gcacgctgaa cagggccgcg gccgaggtaa tcgaccgact gccgggcgtg
      661 aactcgtgca ctgacatcac cggcttcggc ctgatcggcc acgcgtcgga ggtggccaac
      721 gcgagcggcg tgacactcgt gctcgatgcg gcgcaggtgc cgctcttcga cggcgtcaag
      781 gcgctcgtgc gccgcaaccg cagcgccggc atgaagagca atcgcgagca ctttggcagc
      841 ggcgtggcgg tcggcgccgg catcgacgac gatctgctcg atctctgcta tgacccgcag
      901 acctcgggcg gcctgctgat ctttgtcgcc gaggcgcacg cgcccgcggc gctggccgcg
      961 ctgcgcgcgg cgaacgtgcc ggcggtcgag atcggccgcg cggtggctcc caccggtcac
     1021 tggatcgagc tgcgctgacg atggaacgct tcacgatctc gctgtcggag gaactcgcaa
     1081 aggaattcga cgcgctgatc gccacgcgcg gctacgagaa ccgttccgag gcggtgcgcg
     1141 acctgatccg gcgcgagctg gaatcgctgc ggcggacacg cgatccgcac gcgcactgcg
     1201 tggcaaacct gtcctatgtc tacaaccacc acgagcgcga tctcgccgac cggctgatcg
     1261 aactgcagca cgagtaccac gatctgtgcg tatcgacgct gcacgcgcat ctggatcacg
     1321 accactgcat ggaaagcctg atcctgcgcg gccatctgga cgaggtgcag tcgttcgccg
     1381 accggctcgt cgccgagcgc ggcgtgcgcc acgggcagtt gaacgtggtg acggtcgagc
     1441 cgggccgcgc gcaccggcac gggcacggcg cgccgccgca cacgcatttc cggccgcacg
     1501 actgacgccg ctcagtgcgg cggcgggcgc ggcagttcga tctcggccag cacccagcgc
     1561 gcgttgcccg ccagcggctc gaagtcgcga ccggggtggg tcgctgcgag ccattgctcg
     1621 acctcggata tccgcctgcg ctcgacgatc atgaacaccg gaggcggcgg cgccggcgtc
     1681 gtggctgtgc cgatgaatgc cgttgccttg tcgcgcgaat agaagcgcag cgaatgcggc
     1741 acgcggtcgc cccagtacag cagcgggccg ggctcggcga tcccttcctg ccgccacagt
     1801 tcgaccagcg actcggtact gcgatctctc gattcgtagg gcgcccaggc gatccaggcg
     1861 gccgcgaggc tgatccacgc cagcggcagc ccggacatga acgcgcgcgc ccaggccaat
     1921 cgcggcaatc gccgctcgaa cgcatttgcc agcagcaccg ccagcggact gagcatcggc
     1981 agcacgtagg gccagatgat gttgcgggcg aaggtgaaga agagcagtgg caccgccgcg
     2041 catagcagcg cgtacgtgaa ctgcgggtcg gcgtcgcgca gcggcacgcg cagcccggat
     2101 gcgcgcacgc gcgaaacgag cagcgcgatc acgaccggcg cccatacgcc gagtgcaccg
     2161 agcaggaaca gccagatgaa accgcgcggc cgcacgtgcg cggcgccgta caggtcgccg
     2221 cgccagcccg gatcgatgaa gcgcatcacg tgctcgccga gcaggaaata ggacagaaag
     2281 cccggcgtgc gcatttcggc cgcggcgtac cacggcaccc agagtgcggc aaagagcagg
     2341 gtgccgcgca gccacggcag cgcacgccac gccgcgcgca cgcggccggt ggccagcgtc
     2401 cacagcgcaa tcggcaggcc cgcgtagacc atcacgaccg gtcccttcgc gagcatgccg
     2461 agcccggccg cgatgaagaa gccccagcgc cagaaccacc cggtctgccc gcgcagcagc
     2521 gtctgcatgt acgcgaccat catccacgtg ctgcagaagg cgagcgatgg atcggtcatc
     2581 actgcgcccg ccgacgcgta gaacagcacc gacgtggcga ggatcgccgc cgccatcagt
     2641 ccgcgctcga gcgcgagcca cggcgtgccg ttgcttccgc cgtcgcgcgc ccaggaaaac
     2701 accaacgcca gcgtgccgac cgcgaacagg aacgacggca gccgcgccgc cagttcgttc
     2761 acgcccagca gcgccatcga tgcggcgctc agccaggcat acagcggcgg cttggcccag
     2821 aacggctgcc cgggcatctc ctgcggcgtg atccagttgc cggtcgccag catcacgcgc
     2881 gcgatctcgc cgtagcgcgc ctcggtggtg tcggtcagcg cgtaggtgcc gagcgtgaca
     2941 gcgcgtatca ccaaggtggt gaacacgagc gtgaggagaa gcatcacgcg cgcgcgcggg
     3001 tcgagcccca gttcgtcgag cagccggatc tgcatgggtg cctgcgatgg tagcgggtcc
     3061 gcaccgtcga acccgctgcg cttgatcaag cacaaggcat cgatatgaag aaccgtggat
     3121 tattcatacg acgaggcggg cgtcgcccga cctcgacgcg aaagcaagcg atgcacaatc
     3181 tgcccaccga atggaccgcg ctggcggcgt tggtcttcct gctcggcctg aagcacgggt
     3241 tcgacgccga tcacctggcc acgatcgacg ggctgacgcg catcaacgcg cgcgcgcgtc
     3301 ggtcatgggc gcgctactgc ggcgcgctct tttcgctcgg gcacggtgct gtcgtgctgg
     3361 cgattgcgct cggcgtcggg ctggcaagcg cgaagtggga tgcgccgcaa tggctggagg
     3421 ccagcggcgc gtggatctcg atcgccttcc tcgcggcgct cggcatcctg aacctgcacg
     3481 cggtgctggc gacgccgcgg caccagatcg tggcgcccgt tggactgaag ggccggctgc
     3541 tcgggcgact gaatcgcgcg cagcatccgg cgctggtggc gctggtcggc gcgctgttcg
     3601 cgctgtcgtt cgataccgtg agccaggcgg cgctgttcgc gatgaccgcc gcgcagttcg
     3661 gcggcgtgac gcatgcgctg atgctggggc tgctgttcat gctcggcatg atggtgaccg
     3721 acggcatcaa cgggttgtgg atctcgcggt tgatcgcgcg cgccgatcag ttggcactga
     3781 tcgcctcgcg cgtgatgagc ctcgcggtct cgggcgtgag cctgctggtc gcggcgctcg
     3841 gtgcggccaa gcttgcggtt ccggtcgtcg atacgtggag cgacggcaag gaactcgcgt
     3901 tcggtgccgt cgtcgtcgcc gtggtcgcgg gcagctatct ggccgcgcgc gcgctcgcgc
     3961 tgcagccagg cgcggcggcg gaccgccgca cctgatcgca ttacgttgac atgcagccgc
     4021 gccggggccc gttgccggga ccccagccgg ctccgcgctg ccccatgcgc gcgcccggac
     4081 cgtaactgtc gagcaccttc ttctgctcgg ccgtcagcac cgcgtacagg ttggtgcgca
     4141 gcgtgtgcag ttcctgcgcc gcttccgcgt ggtgcttcgc catcgtggcg tggaactcgc
     4201 tcatcgcctg cgcgtcgccc atgcggttct gcatttcggt gcgcagcttg gcgcgcgcct
     4261 cggcctcgct ccgcaccttg gcggcccagg cgtcgaacgc ggcgacctga ttcggcgcga
     4321 gcttcagttc gttgcgcagc gcctcgatgc gcgcctgcgc acgctcgggc tgcgcgcctg
     4381 ccatcggggc ggagcgatgc gcgcccgggc cggggccgta ccccggtccc tgcgacagcg
     4441 cgggcacggc cgccccgatc agaacggccg cgaccgcggc cgatatccat gtgcgtctgc
     4501 tgttcatccg attctccttt gcgtctgcct gactgcaccg tcacggtagg cgcatggcgc
     4561 ggcaccggtg taaccgcgca ttgcggtgcg ggcgcgccgt aacacattga gagcagacgt
     4621 gatcgagatc acgcacgcgc gaatggcgag aggtccgcga gggcggtgat aaagtacgcg
     4681 gccgcgcgag ccggcgcgaa tcgatccgag gttgtttgtg aagaactggt ttttccggtg
     4741 tgtgccggcc gtgctggtgt ccctgctcgt cgcctgcggc ggcagcaatg gcggctcggc
     4801 ctcgccgccg accaacgtga ccacccagtc gggcgacggc tggatccggg tcaactggga
     4861 ttcggcgggc ggtctcgaat actgggtgtt caccgcgctc gatgcgtcgc tgacgacgct
     4921 gaactggctg aacctcgtcg gcggcgccgc cttcatcaat gtgacgccac cgctgacgct
     4981 gtgcagccag accaacggac tgcagcgctg gttcaccgtc aacagccgca gcggctccga
     5041 ccccggcggg cccggcagcg cggtcgtcag cgccgttccg agcgcggcag gcgccaactg
     5101 gaccggcggc gccacgctcg gcgacgacct tgcgggcatc ggcttcggcg tgctcacgac
     5161 ctgccttcgc accgggctgc cgaccggtgt gctgaccgcg gtcgggccgg gcgcagtcat
     5221 ctattcgagc agcgacgccg gcgcgaactg gacgcggcgc gccgccccga gcggcttcac
     5281 cggcgacctg aacgccgtcg cgaactacac cgccaatccg aactcgacca cggccagccc
     5341 cggcatgcgc acggtcgcgg tcggcgccgg cggcgcgtcg atctacagca ccgacagcag
     5401 cgtcacctgg acagtcggcc gtgcgccgag cgcgtcgagc gccacgctgc gcggcctcgt
     5461 cacggtgagc agcgcgttcg tcgcggtcgg cgacggcggg acgatccagt cgtccagcga
     5521 cggcatcacg tggaacgcac tgccgtcgaa cacgaccacg aacctgcgcg ggctggcatt
     5581 cggcaactcg cgctatgtcg cggtcggcga tggcggtacg ctcgtcacca gcaccgacag
     5641 cggcgtgtca tggaccgtgc agacgatcgc cggcgccggc aacctgcgcg cggtggcata
     5701 cggcagcaac aacaacaaca ccgacaacgg cggcacgctc ctgatcaaca ccttcgttgc
     5761 ggtggccgac aatggcagcg cggtcgtcag ccacgaaggt ggcgtgaact ggtccgtcgt
     5821 cgccgtgccc ggcgcagggg aac
//
LOCUS       DBNHBY010000258         1775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_218670, whole genome shotgun sequence.
ACCESSION   DBNHBY010000258 DBNHBY010000000
VERSION     DBNHBY010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_218670"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="glmU"
                     /locus_tag="AC16HB_06335"
     CDS             complement(<1..1126)
                     /gene="glmU"
                     /locus_tag="AC16HB_06335"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR5606443.1"
                     /translation="MNIVILAAGQGKRMRSALPKVLHPVAGQPMLAHVIATAEAVARD
                     HAVTANVVAVIGHGADQVRAAFSDRADLRFVLQQPQLGTGHAVQQAVPWLRDDEPTLV
                     LYGDVPLVRTETLNRLLDGAAGEVGLLTVELADPTGYGRVVRDGRGNVQRIVEQKDAS
                     ASERALREVNTGILVAPTARLKSWLAALSNDNAQQEYYLTDVIARAVREGVRVAAIAA
                     HDADETLGVNSKAQLALVERIAQRRAAEVLLSAGVTLADPARIDVRGEISTGADVSID
                     VGCVFEGRVHLADGARIGPYCVLRDVTIGARTQLIAFTYLESTTVGADARVGPYSRTR
                     PGAVLDDEVHIGNFVEVKASRLGRGAKANHLAYVGDAVVGA"
     gene            1223..>1775
                     /locus_tag="AC16HB_06340"
     CDS             1223..>1775
                     /locus_tag="AC16HB_06340"
                     /inference="COORDINATES: protein motif:HMM:NF039714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HIR5606444.1"
                     /translation="MNARRFVLVSVALLVAACSSLKLGYNNADTLLLYSLDSYLDLDN
                     RQEELAGERVRRLLEWHRATQLAGYAQLLGDAQRTLDGRVGADDVAALQRQMNDKLAA
                     IGEQVAPDVAALALTLTPAQIDHFARKLAKESSVARRQLARFAGGRESIDERVKRYSN
                     RAESWFGSVTAEQTQILHAALATA"
BASE COUNT          277 a          632 c          591 g          275 t
ORIGIN      
        1 gtgcgcccac cacggcatcg ccgacgtagg cgaggtgatt cgccttcgcg ccgcggccga
       61 gccggctcgc cttcacttcg acgaagtttc cgatgtgcac ctcgtcgtcg agcacggcgc
      121 ccggccgcgt acgcgagtaa ggaccgaccc gcgcgtcggc gcccacagtc gtgctctcca
      181 ggtaggtgaa tgcgatcaac tgcgtgcgcg caccgatcgt gacgtcgcgc agcacgcagt
      241 acggtccaat gcgcgcgccg tcggccagat gcacgcggcc ctcgaacacg cagccaacgt
      301 cgatcgacac gtcggcgccc gtgctgatct cgccgcgcac gtcgatgcgc gccgggtccg
      361 ccagcgtgac gccggccgaa agcagcacct cggccgctct gcgctgcgcg atgcgctcga
      421 ccagcgcaag ctgggccttg ctgttcacgc cgagcgtctc gtcggcgtcg tgcgccgcga
      481 tcgccgcgac acgcacgcct tcgcgcaccg cccgggcgat cacatcggtc aggtagtact
      541 cctgctgcgc gttgtcgttc gacagcgcag cgagccagct tttcagccgt gcggtcggag
      601 ccacgaggat gccggtgttg acctcccgca acgcgcgttc gctcgcgctt gcatccttct
      661 gctcgacgat ccgttgcacg ttgccccgtc cgtcgcgcac gacgcggccg tagccggtgg
      721 ggtcggcgag ttcgaccgtc agcagtccga cctcaccggc cgcgccgtcg agcagccggt
      781 tcaaggtttc ggtccgcacc agcggcacgt cgccgtacag caccagcgtc ggctcgtcgt
      841 cgcgcaacca cggcacggcc tgctgcaccg catgtccggt acccaattgc ggctgctgca
      901 gaacaaagcg cagatcggca cggtcagaga aggcggcccg gacctggtcg gcgccgtgtc
      961 caatgacggc gacgacgttc gcggtcacgg cgtgatcgcg cgccacggcc tcggcggtcg
     1021 cgatcacgtg cgctagcatc ggttgacccg cgaccggatg cagcaccttg ggcagcgcgg
     1081 agcgcatgcg cttgccctgg ccagcggcca ggatgacgat gttcatcgtg cgcaacagga
     1141 tggcactgcg gttatgcgag ttatcacaga gcaaatcgat cgtagcaccg gggacgcgct
     1201 gcgctgcccc ggtcgcctcg gcttgaacgc gcgccgcttc gtgctggtca gcgtcgcgct
     1261 gctggtggcc gcgtgctcgt cgctgaaact cggctacaac aacgccgaca cgctgctgct
     1321 gtattcgctc gacagctatc tcgatctcga caacaggcag gaggaactgg cgggcgaacg
     1381 cgtgcgccgg ctgctggagt ggcatcgggc cacgcagctt gcaggctatg cccagctgct
     1441 gggagacgca cagcgcactc tggacgggcg cgtcggcgcc gacgacgtgg cggcactgca
     1501 gcggcagatg aacgacaagc tcgcggcaat cggcgaacag gtcgcgcccg acgtcgccgc
     1561 gctggcgctg acgctgacgc cggcgcagat cgaccacttc gcccgcaagc tggccaagga
     1621 gtcgtctgtc gcgcggcgtc agctcgcgcg cttcgccggc ggccgcgaat cgatcgacga
     1681 gcgcgtcaag cgctattcga accgggccga atcctggttc ggctccgtga ccgccgaaca
     1741 gacgcagatc ctgcacgctg cgctcgccac agcga
//
LOCUS       DBNHBY010000259         4498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_624070, whole genome shotgun sequence.
ACCESSION   DBNHBY010000259 DBNHBY010000000
VERSION     DBNHBY010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_624070"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC16HB_06345"
     CDS             complement(<1..567)
                     /locus_tag="AC16HB_06345"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5606445.1"
                     /translation="MRPRAARRAGSTEGSRNTNCGARKLGLRSDWDFVLHLPLRYEDE
                     TRVTPIAELRAGDDAQIEGEIVACEVTRRARRQLNVRMADASGELLLRFLHFYPSQLK
                     SLAVGRRVRAFGTVRGGLTGHEMVHPRVRGADAGAALPASLTPIYSTTAGVPQSWLRK
                     RIERALRDVDLADTLPDTLVARLRLLPLR"
     gene            complement(573..953)
                     /locus_tag="AC16HB_06350"
     CDS             complement(573..953)
                     /locus_tag="AC16HB_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR5606446.1"
                     /translation="MNKEIVATKAAPAAIGPYSQAVKTGLVVFTSGQIGLDPRTTELV
                     GDDFEAQVRQSFRNLAAVAQAAGGSLANAVKFTLFLTDLSKFSIANSVMAEMVPQPFP
                     ARSTVGVASLPKGAQFEVDAVLVL"
     gene            1245..1727
                     /locus_tag="AC16HB_06355"
     CDS             1245..1727
                     /locus_tag="AC16HB_06355"
                     /inference="COORDINATES: protein motif:HMM:NF037735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HIR5606447.1"
                     /translation="MILFLDFDGVLHPDPCYDGRRMFEHVPLLAAVLARFPETGIVLT
                     TSWRSDRSLAQLREPLGALAARVVGATPHFSSFRAPARLQPYTRQAECEQWLIATSAT
                     GREWIALDDRASLFAPDCDRLIACDSTRGLDESSAGRLCFALARARRVAEQRRESLSA
                     "
     gene            complement(1884..2978)
                     /locus_tag="AC16HB_06360"
     CDS             complement(1884..2978)
                     /locus_tag="AC16HB_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5606448.1"
                     /translation="MLTRRQLIQAAGAGALGGGSLAALAQGGSAEPLKIGFVYVSPIG
                     EAGWTFQHDTGRKEMEKALAGRVQTRFIESVQEGPDAERVFRELAASGHKLVYGTSFG
                     YMNYMEKVARQFPNTIFEHATGYKTAKNLGVYNARFYEGRYLCGVIAGRMTQSNTLGY
                     VAAFPIPEVLQGINAFIRGARSVNPKAEVRVVWVNSWYDPGKEREAANTLLSQKADIV
                     THHTDSTAVVQAAEARGAMAFGYHSDMSKFGPKAHLTACTHNWGDYYTKSARSVLDGT
                     WKSGSVWGGMREGFIRLAPLNPIVPAAVKAEVARLEAGLKAGSFHPFAGPVKDQSGRE
                     VVPAGRSMTDDDLGKMNYYVDGVASQLPKS"
     gene            complement(3023..3955)
                     /locus_tag="AC16HB_06365"
     CDS             complement(3023..3955)
                     /locus_tag="AC16HB_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606449.1"
                     /translation="MEHGILTSIVAATILSGTPLVIAALGQLVAERSGVLNLGAEGTM
                     AMGAVAAFAVALHTGSAWSGVLAGMAAGIAMALIFAVLTLTLMANQVAAGLALTIFGM
                     GLSAFIGKPYESHSLPEIPPIQVPLLSDLPFFGPALFGQKSLVYFSWLLFAAVTWFLY
                     RSRAGLMLRAIGESPSSAHAIGHPVIRVRYLAALFGGAMCGIAGAFLSVYYTPMWAEG
                     MVAGRGWIALALVVFATWRPLRVLAGAYLFGGVMIMQLFLQGSGAQISIPAQFLSSLP
                     YWATIAVLVLISRNPNMIRLHSPASLGQPYRPDA"
     gene            complement(3966..>4498)
                     /locus_tag="AC16HB_06370"
     CDS             complement(3966..>4498)
                     /locus_tag="AC16HB_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5606450.1"
                     /translation="IEGTRLNLAFVVALLVLVAGYVFMQRSFYGFQMLVAGEAAAAAR
                     YAGYSAKGVVWVGLLVGGATAGIAGMAEVAGPMAQLTPAVSANYGFAAIIVAFVGRLN
                     SIGILLASLVMSLLYLGGEQVQQYLNLPSSISLVFQGMLLFFLLGADLFINFRLRRRA
                     AQSAPVAASAVAKPVA"
BASE COUNT          751 a         1589 c         1473 g          685 t
ORIGIN      
        1 gcgcagcggc agcagacgca gtcgcgcgac gagcgtgtcg ggtagcgtgt ccgcgagatc
       61 gacatcgcgc agcgcgcgct cgatccgctt gcgcagccac gattgcggta cgccggccgt
      121 cgtcgaatag atcggcgtca gggacgccgg cagcgcggcg cccgcatcgg cgccccgcac
      181 gcgcgggtgc accatctcat gcccggtcag tccgccgcgc acggtgccga acgcgcgcac
      241 gcggcggccg accgcgaggc tcttcagctg tgacgggtag aagtgcagaa accgcagcag
      301 cagctcgccg ctggcgtcgg ccatccgcac gttgagctgc cggcgcgcgc gccgcgtgac
      361 ctcgcacgcc acgatctcgc cttcgatctg cgcatcgtca cccgcccgca gctcggcgat
      421 cggcgtgaca cgtgtctcgt cctcgtagcg caacggcagg tgcagcacga agtcccagtc
      481 gctgcgcagg ccgagcttgc gtgcaccgca atttgtgttc cgtgatcctt ccgtcgaacc
      541 ggctcgacgg gccgcgcgcg gccgcaccga cgtcagagca cgagtaccgc atcgacctcg
      601 aactgcgcgc ccttgggcag gcttgccacg ccgacggtcg agcgcgccgg gaacggctgc
      661 gggaccatct ctgccatcac gctgttggcg atggagaact tggaaagatc ggtcaggaac
      721 agcgtgaact tcaccgcgtt cgcgagcgat ccgccggcgg cctgcgccac tgccgccaga
      781 ttgcggaacg actgccgcac ctgggcctcg aagtcgtcgc cgacgagctc ggtcgtccgc
      841 ggatccagcc cgatctggcc ggacgtgaac accacgagcc ccgttttcac cgcctgcgaa
      901 tacggcccga tcgcggcggg ggctgctttc gtcgcaacga tttccttgtt catgtggtgg
      961 ctccgcgcgt caacacgggc agtctagcaa cggcggcgcg cgggaaaaga aagcgcgggc
     1021 acaactcggg ggaagccggc ccgcgcatgc ccctgacggg gcgtcggagg agacaacaca
     1081 cgtccgaccc aagcgtcctg acaaagttcc catctcgcgg atggcatgaa cggggcattg
     1141 cgtacgatga acgacccgca gagcgcatcg ccccgtgcaa atgtccgata ggcacgtgac
     1201 gtcgcgcctg tcatagtcgc cccagcatca ccgagtgggc gatcgtgatc ctgttcctcg
     1261 acttcgacgg cgtactgcat cccgacccgt gttatgacgg gcggcggatg ttcgagcacg
     1321 tgccgctgct cgccgcggta ctggcccgtt tccccgagac cggaatcgtc ctgacgacga
     1381 gctggcgcag cgatcgcagc ctcgcgcaat tgcgcgagcc tttgggcgca ctggccgcgc
     1441 gcgtggtcgg cgcgacgccg cacttctcct cgttccgcgc gccggcgcgg ctgcagccct
     1501 atacgcgcca ggctgaatgc gaacaatggt tgatcgcaac cagcgccacc ggccgcgaat
     1561 ggatcgcgct cgacgatcgc gcctccctgt tcgcgcccga ctgcgaccgg ctgatcgcat
     1621 gcgactcgac gcgtgggctg gacgaatcga gcgccggccg cctgtgcttc gcgctcgcgc
     1681 gtgcgcgccg cgtcgccgag cagcggcgcg agtccctgtc cgcgtgacac ggcgccggac
     1741 ggcgccaccc ggttacgatc cgctgcgtcg aagtgggcgc agcgcgcggg tgcgctgcgc
     1801 atgaccgaca cacgtcagcg cgaaccgggc ctctcggccc cgacttctgc ctgccgatgc
     1861 gacgcgacgc tcgcgccgcg tcttcaactc ttcggcaact ggctcgccac gccgtcgacg
     1921 tagtagttca tcttgccgag atcgtcgtcg gtcatgctgc ggccggccgg cacgacttcc
     1981 ctgcccgact ggtccttcac cggtcccgcg aacggatgga acgagccggc cttcagtccc
     2041 gcctcgagcc tggcgacctc ggccttcacc gctgcgggaa cgatcgggtt cagcggcgcc
     2101 aggcggatga agccttctct catgccgccc cacacgctgc cgctcttcca cgttccgtcg
     2161 agcaccgacc gcgcgctctt cgtgtagtag tcgccccagt tgtgcgtgca tgcggtcagg
     2221 tgcgccttcg ggccgaactt gctcatgtcc gagtggtagc cgaacgccat cgcgccgcgc
     2281 gcctcggccg cctgcacgac cgcggtcgag tcggtgtgat gggtgacgat gtcggccttc
     2341 tgggacagca gcgtgttcgc cgcctcgcgc tccttccccg ggtcgtacca ggagttgacc
     2401 cacaccacgc gcacctcggc cttcgggttc acgctgcgcg cgccgcggat gaacgcattg
     2461 atcccctgca gtacttcggg gatcggaaac gccgccacgt agccgagggt gttcgattgg
     2521 gtcatgcgcc ccgcgatcac gccgcacagg tagcggccct cgtagaagcg cgcgttgtag
     2581 acgccgaggt tcttcgcggt cttgtatccg gtcgcgtgct cgaagatcgt gttcggaaac
     2641 tgccgggcga ccttttccat gtagttcatg tagccgaagc tggtgccgta gaccagcttg
     2701 tggccgctcg cggccagttc gcggaacacg cgttcggcgt ccggaccttc ctgcacgctt
     2761 tcgatgaaac gggtctgcac gcgaccggcc agggccttct ccatctcctt gcggcccgtg
     2821 tcgtgctgga acgtccatcc ggcctcgccg atcgggctga cgtagacgaa gccgatcttc
     2881 agcggctccg cgctcccgcc ctgcgcgagc gcggccaggc tgccgcctcc gagcgcgccc
     2941 gcacccgcgg cctggatcag ttgtctgcgt gtcaacatgc tcgaatctcc gagaggcagg
     3001 tatgcgtcgc tgggggattg cgctacgcat ccgggcgata gggctggccg agcgacgccg
     3061 gcgaatgcag ccggatcatg ttcggattgc gcgagatcag caccagcacg gcgatggtcg
     3121 cccagtaagg cagggaagag aggaactgcg ccgggatgct gatctgggcg ccggacccct
     3181 gcaggaagag ctgcatgatc atcacgccgc cgaagagata cgcacccgcg agcacgcgca
     3241 gcggccgcca ggtcgcgaac accacgagcg cgagcgcgat ccagccgcgc ccggcgacca
     3301 ttccctcggc ccacatcggc gtgtagtaga cggatagaaa cgcgccggcg atgccgcaca
     3361 tcgcgccgcc gaacagcgcc gcgagatagc gcacgcggat gaccgggtgc ccgatcgcat
     3421 gcgcggacga cggcgattcg ccgatcgcgc gcagcatcag cccggcgcgg ctgcgataca
     3481 ggaaccacgt caccgcggcg aacagcagcc acgagaagta gaccagcgac ttctggccga
     3541 acagtgccgg tccgaagaat ggcaggtcgg aaagcagagg aacctggatc ggcgggattt
     3601 ccggcagaga atgcgattcg tacggcttgc cgatgaacgc cgacagtccc atgccgaaga
     3661 tggtgagcgc aagtcccgcc gcgacctgat tggccatcag ggtcagcgtc agtaccgcga
     3721 agatcagcgc catcgcgatg ccagccgcca tgcccgcgag cacgccgctc cacgcgctgc
     3781 cggtgtgcag cgcgaccgca aacgccgcga ccgcgcccat cgccatcgtg ccctcggcac
     3841 cgagattgag cacgcccgag cgctctgcca ccagctggcc gagcgcggcg atcaccagcg
     3901 gcgtgcccga caggatggtg gctgcgacga tcgacgtgag gatgccgtgc tccatcacgc
     3961 gcctcttacg ccacgggctt cgcaaccgcg gacgcggcga cgggcgctga ctgcgccgcg
     4021 cggcgtctca gccggaaatt gatgaacagg tctgcgccga gcaggaagaa cagcagcatg
     4081 ccctggaaca cgagcgatat cgacgacggc aggttcaggt actgctggac ctgttcgccg
     4141 cccagataca gcagcgacat caccaggctc gcgagcagga tgccgatgga attcagccgg
     4201 cccacgaacg cgacgatgat cgccgcgaag ccgtaattgg ccgataccgc cggagtcagc
     4261 tgcgccatcg gtccggcgac ctccgccatg ccggcgatgc cggccgttgc gccgccgacc
     4321 agcaggccga cccacaccac ccccttcgcc gagtagcccg catagcgcgc cgcggctgcg
     4381 gcctcgccgg cgaccagcat ctggaagccg tagaagctgc gctgcatgaa gacgtagccg
     4441 gcgacgagca ccagcaacgc gaccacgaac gccaggttca gtcgtgtccc ctcgatca
//
LOCUS       DBNHBY010000260         4181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1504330, whole genome shotgun sequence.
ACCESSION   DBNHBY010000260 DBNHBY010000000
VERSION     DBNHBY010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1504330"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16HB_06375"
     CDS             <1..265
                     /locus_tag="AC16HB_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606451.1"
                     /translation="AEAAGFRLAGALTMSPAPDAVDAAFTHALASAHVVVVDAHCAAR
                     LALARLEAALAKTHPLVLIAPGDPPHPLDPATRVARQLGLERF"
     gene            375..980
                     /locus_tag="AC16HB_06380"
     CDS             375..980
                     /locus_tag="AC16HB_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606452.1"
                     /translation="MNLKQRTSALLQVVDAYRARRCAELLEPASEQARQLQREALRTA
                     RSRVHTAIEEQRKRVAAEIGAAEARLATERRLLAQRSATATLAQAWEPLRAALLARWQ
                     DASTRARWVETHLARAPAALPRSQWQIHHPPDWPPAEREAVAARLAAQGIGAEMIADA
                     SIAAGLRVRAGNNALDFTLDGLLAERAALEGRLLQLLVETP"
     gene            977..2758
                     /locus_tag="AC16HB_06385"
     CDS             977..2758
                     /locus_tag="AC16HB_06385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR5606453.1"
                     /translation="MSTATISWISGPVLRARTTGEFRLREAIRVGPEQLLGEVIRVAE
                     NEIVAQVYEDTTGLRPGVAVEGSGELLSIRVGPHLLGRIFDGLLRPLAQGARHILPGA
                     AAHDDARFDFQPSVARGDALARGAVFGEVQGGRRVLLPPDAAGSVDRIATAGNYSDTE
                     PLLILRQPDGRTREIALSHSWPVRTPRPVIERLPSDEPLVTGQRILDSLFPIALGGKG
                     AIPGGFGTGKTILLEALAKGCSADVIVYLGCGERGNEMAGVLEEFPQLVDPRTGRPLM
                     ERTVIIANTSNMPVAAREASIYSGVTVAEYFRDQGLHVALMADSTSRWAEALREVSGR
                     FGELPGEGGYPNYLSSRLADFYERAAKVRTLAGDIGSVTLLGSISPPSGDFSEPVTSH
                     TKRYVRSFWALDARRAQARFYPAVNPLQSYSEDSLDMAVWWQKLYANVPTARTRAASG
                     ENEWLELRRKFLTLLDEQARLERMARIIGKDALPARQRLALACAELVNEAFLRQSAFS
                     PIDRTCSPQRQACLMRLLAHFIELAQHALAHGASPEAIAALDVMRPLARMSEDIGEDA
                     LDAFAALEARVEAAFAALVKTEAASAV"
     gene            2828..>4181
                     /locus_tag="AC16HB_06390"
     CDS             2828..>4181
                     /locus_tag="AC16HB_06390"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR5606454.1"
                     /translation="MRSRITVHQAAVKIDGPLLFLRRTLEIGLNEAVEVLDVRGRSRL
                     GRVAALDEQFVTIELLEPSAGLGLTETVVRFLGTPIEFGVGPGVLGRVFNGVGQVIDG
                     GPPLAVKKQLRVDGLPLNPVARATPRAFIESGVAAIDLMNSLVRGQKLPIFSGGGLPH
                     DRIAVEIAAHARLLGAEANEFAIVFCGIGVPYTTADYFRRALEGSGALERAALFLNLA
                     SDSSTQRLLTPRFALTAAEYLAFEEGLHVLVIVTDLTNYCEALREVAGAKGEVPGRKG
                     FPGYMYSDLATLYERAGCVHGAKGTLTQLPILTMPSDDIGHPIPDLSGYITEGQIVLS
                     RELDRAGVYPPINVLPSLSRLMKDGTGKGYTHPDHPALASQLYAAYAHATQARVLASV
                     VGEEGLSETDRRYLAFGTEFEQRLVNQAGARTLVQSMEAGWELLRALPVAELTRLSDA
                     Q"
BASE COUNT          607 a         1448 c         1537 g          589 t
ORIGIN      
        1 ggcggaggcg gcggggtttc gcctcgccgg cgcgctgacg atgtcgcctg cgcccgatgc
       61 ggtcgatgca gcattcacgc acgcgctggc ctcggcgcac gtggtggtcg tcgacgcgca
      121 ctgcgcggcc cgccttgcgc tcgcgcggct cgaagcggcg ctggcgaaga cgcacccgct
      181 ggtgctgatc gcgcccggcg atccaccgca tccgctcgac cccgccacgc gcgtggcacg
      241 tcagctcggg ctggagagat tctgagcatg cgcgaagcgc ctgcgatcgc gagtccgacg
      301 cacggccgcc cgaagacggg cgagaccccc gcggggagtc gcgcgccaca tgtggcgcgc
      361 ggggggcgcg ccagatgaat ctcaagcaaa gaacctcggc gctgctgcag gtcgtcgacg
      421 cgtatcgcgc gcgccgttgc gccgagttgc tcgagcccgc gtccgagcag gcgcggcagc
      481 tgcagcgcga ggcgctgcgc accgcgcgca gccgcgtgca caccgcgatc gaggaacaac
      541 gcaagcgcgt tgcggccgag atcggcgccg ccgaggcgcg gctggcaacc gagcgccggc
      601 tgctcgcgca gcgcagcgcg acggccacgc tggcgcaggc gtgggagccg ttgcgcgccg
      661 ccttgctggc gcgctggcag gacgcatcga cgcgcgcccg ctgggtcgag acgcatctgg
      721 cgcgcgcgcc ggccgcgctg ccgcggtcgc agtggcagat ccatcatccg cccgattggc
      781 cgccggcgga gcgcgaagcc gtggcggcgc ggctcgccgc gcagggtatc ggcgccgaga
      841 tgatcgccga cgcgtcgatc gccgcgggac tgcgcgtgcg cgccggcaac aacgcgctcg
      901 acttcacgct cgacggcctg ctggccgagc gcgcggcgct cgaaggccgg ctgctgcaac
      961 ttctggtcga aacgccgtga gcaccgcgac catctcctgg atcagcggcc cggtcctgcg
     1021 cgcgcgaacg acgggcgagt tccggctgcg cgaggcgatc cgcgtcgggc ccgagcaact
     1081 gctcggcgaa gtgattcgcg tcgcagagaa cgagatcgtc gcgcaggtgt acgaggacac
     1141 gaccgggctg cgtcctggcg tggcggtcga aggcagcggc gaattgctgt cgatccgcgt
     1201 cgggccgcac ctgctcggcc gcatcttcga cgggctgctg cggccgctgg cgcagggcgc
     1261 gcggcacatc ctgcccggag ccgcggcgca cgacgatgca cgtttcgatt tccagccctc
     1321 ggtggcgcgc ggcgatgcgc tggcgcgcgg cgcggtgttc ggcgaggtgc agggcggccg
     1381 gcgcgtgctg ctgccgccgg acgctgccgg cagcgtcgac cgcatcgcga ccgccggcaa
     1441 ctacagcgac accgaaccgc tgctgatcct gcggcagccc gacggccgca cgcgcgagat
     1501 cgcgctgagc cactcgtggc ccgtgcgcac gccgcggccg gtgatcgagc gcctgccatc
     1561 ggacgaaccg ctcgtcaccg gacagcgcat cctcgattcg ctgttcccga tcgcactcgg
     1621 cggcaagggt gcgattcccg gcggctttgg caccggcaag acgatcctgc tcgaggcgct
     1681 cgccaagggg tgcagcgccg acgtgatcgt ctatctaggc tgcggcgagc gcggcaacga
     1741 gatggccggg gtgctggagg aattcccgca actcgtcgac ccgcggaccg ggcggccgct
     1801 gatggagcgc acggtgatca tcgccaacac gtcgaacatg ccggtcgcgg cgcgcgaggc
     1861 gagcatctac tcgggagtga cggtcgctga gtatttccgc gaccaggggc tgcacgtggc
     1921 gctgatggcg gattccacat cgcgctgggc cgaggcgctg cgcgaggtgt cgggccgctt
     1981 cggcgaactg cccggcgagg gcggctatcc gaactacctg tcgtcgcggc tggcggactt
     2041 ctacgagcgc gccgccaagg tgcgcacgct cgccggcgac atcgggtcgg tcacgctgct
     2101 gggctcgatt tcgccgccat cgggcgactt ctccgagccg gtgaccagcc acaccaaacg
     2161 ctatgtgcgc agcttctggg cgctggacgc gcggcgcgcg caggcgcgct tctatccggc
     2221 ggtcaatccg ctgcagtcgt attcggagga ttcgctcgac atggcggtgt ggtggcagaa
     2281 gctctacgcc aacgtgccga ccgcgcgcac gcgcgccgcc agcggcgaga acgaatggct
     2341 cgagttgcgc cgcaagttcc tcacgctgct cgacgagcag gcgcggctcg aacggatggc
     2401 gcgcatcatc ggcaaggacg cgctgccggc gcggcagcgc ctggcgctcg cgtgcgcgga
     2461 actcgtcaac gaggccttcc tgcgccagtc ggcgttctcg ccgatcgacc gcacctgcag
     2521 tccgcagcgg caggcgtgcc tgatgcggct gctggcgcac ttcatcgagc tggcacagca
     2581 cgcgcttgcg cacggtgcaa gccccgaggc gatcgcggca ctcgacgtga tgcggccgct
     2641 ggcgcggatg agcgaggaca tcggcgagga cgcgctcgat gcgttcgccg cgctcgaagc
     2701 gcgcgtcgag gccgcgttcg cagcgctggt gaagacggag gctgcgagtg cggtttgagc
     2761 ggcatacgag gccgatccat acgcgccgac gcctctcccg gggcgagccg ggcggagggc
     2821 gagggacatg agatcccgca tcaccgtcca tcaggcggcg gtcaagatcg acggcccgct
     2881 gctgttcctg cgccgtacgc tcgagatcgg attgaacgaa gcggtcgagg tgctcgacgt
     2941 ccgcggccga tcgaggctcg ggcgcgtggc cgcgctcgac gagcagttcg tgacgatcga
     3001 actgctcgag ccgagcgcgg ggctgggcct caccgagacc gtggtgcgct tcctcggcac
     3061 gccgatcgag ttcggcgtcg gccccggcgt gctgggccgc gtgttcaacg gcgtcgggca
     3121 ggtcatcgac ggcgggccgc cgcttgcggt gaagaagcaa ctgcgtgtcg atggattgcc
     3181 gctgaatccc gtcgcgcggg ccacgccgcg cgcattcatc gaatccggcg tggccgcgat
     3241 cgacctgatg aacagcctgg tgcgcgggca gaagctgccg atcttctccg gcggcgggct
     3301 gccgcacgac cgcattgcgg tcgagatcgc cgcgcacgcg cggctgctcg gcgcggaggc
     3361 gaacgaattc gcgatcgtgt tctgcggcat cggcgtgccg tacaccaccg cggactactt
     3421 ccgccgcgcg ctcgagggca gcggtgccct ggagcgcgcg gcgctgttcc tgaacctcgc
     3481 cagcgattcg tcgacgcagc ggctgctgac gccgcgcttc gcactgaccg cggccgagta
     3541 cctcgcgttc gaggaagggc tgcacgtgct ggtgatcgtc accgatctca ccaactactg
     3601 cgaggcgctg cgcgaagtcg cgggcgcgaa gggcgaggtg ccggggcgca agggcttccc
     3661 cggctacatg tattcggacc tcgcgacgct gtacgagcgc gccggttgcg tgcacggcgc
     3721 gaagggaacg ctcacacaat tgccgatcct gacgatgccg agcgacgaca tcggccaccc
     3781 gatccccgat ctgtccggct acatcaccga gggacagatc gtgctgtcgc gcgaactcga
     3841 ccgcgccggc gtgtatccgc cgatcaatgt gctgccgagc ctgtcgcggc tgatgaagga
     3901 cggcaccggc aagggctaca cgcacccgga ccacccggcg ctcgcgagcc agctctacgc
     3961 cgcgtacgcg cacgcgacgc aggcgcgcgt gctcgcgagc gtcgtcggcg aggaaggact
     4021 ttcggaaacc gatcggcgct acctcgcctt cggcaccgag ttcgagcagc ggctggtgaa
     4081 tcaggccggc gcgcgcacgc tggtgcagag catggaagcg ggctgggaac tgctgcgcgc
     4141 gctgccggtc gcggagctca cgcggctgtc cgacgcgcag a
//
LOCUS       DBNHBY010000261         4358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1504331, whole genome shotgun sequence.
ACCESSION   DBNHBY010000261 DBNHBY010000000
VERSION     DBNHBY010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1504331"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2739
                     /locus_tag="AC16HB_06395"
     CDS             <1..2739
                     /locus_tag="AC16HB_06395"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5606455.1"
                     /translation="EALKPALRAVLARANGVDSAQWMGTLPHTASAATLLDETDLKPD
                     AVVGPYRLIREIGTGGMGAVWLAERADGTLKRQVALKLPRAIWSPGLAQRMQRERDIL
                     AALEHPHIARLYDAGTDAQGRPFLALEYVEGQPIDAYCRARTLSIKARLQLLLQVAHA
                     VAFAHSRLVIHRDLKPSNILVTADGQVRLLDFGIAKLMEGERTAETQLTQIAGRALTL
                     DYASPEQIKGEPIGTASDVYSLGVVAYELLAGAKPYKLKRGSAAELEETIAAVDAPLA
                     SATATNTQTRKELKGDLDAILNKALKKNPAERYATVDAFAQDIERYLAGHRVVARPDS
                     LAYRLTRFAKRYRTPLIAAAIAIAAFGLAIGVGATALVILALLLGLGAALWQARKARE
                     QTRIAQQQRDAARKEAHRAQAVQGFLVKVFELNSSAQADPIRARQTTVREVLDLGAAE
                     IPQTLKDAPEAQLEVMHVLSSMYLDLALGDQAVALMQSGVQVARRTFGPYGTQLAEAL
                     LWYARGLHQEARRTEIPPLLEEARAILDREGESTSFLRAALLLGTAHYLRDESLSAAR
                     RSADGLVAFLRAHHPERPALIAALRLAVRTRLFAFEHAGAKELAQQAVGQARGLGPTR
                     AAWLVGALGDLGETQWLQAEFDAAENSYREAVATALQANGPDHPRTLGARATFAALLL
                     NTGRRDEGESIAADVLQRIEQAELKLGRGHAVEIRSILAVARWELGYPADAEGVIEAH
                     VEKLRTTHPNSGTLAFRLRTLAEIKIALGLHDEAERLLRQSEAVWHRFAGPDRPQEGD
                     RQFAMTRVRLLNAKGEHGSALGQLQTMPRLDPTAPRLDMSLLLIELERAVTALDIGRV
                     DDGAASARTVVQLIQRFAGGRLPLIEARALMLLERAALMTGGMAAEHGNRQCARWT"
     gene            complement(2803..3201)
                     /gene="arfB"
                     /locus_tag="AC16HB_06400"
     CDS             complement(2803..3201)
                     /gene="arfB"
                     /locus_tag="AC16HB_06400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR5606456.1"
                     /translation="MRHVFADGEVEFSAVRAQGPGGQNVNKVATAVQLRFNVHTSGLP
                     LAVKARLLALNDSRITKDGEVVIKAQSARSQELNKADALVRLTELIEQAEHVPKARRA
                     TKPTYASRLRRLAGKSKRSEIKAGRGRITE"
     gene            complement(3403..3933)
                     /locus_tag="AC16HB_06405"
     CDS             complement(3403..3933)
                     /locus_tag="AC16HB_06405"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5606457.1"
                     /translation="MAKLVFGMNQSLDGYVDHLAFAPSPALFRHFIEEAQRQAGSVYG
                     RMMYEVMRYWDDDHPEWDAEEHAFAAAWRSQPKWVVSRSLKSVGPNARLVEDDLARAI
                     REMKAERDGEIEVAGPNLAHSLAELGLIDEYRIYLHPVVLGHGKPYFAGPRPRLRLMT
                     HDRIGEDVIRLTYIPA"
     gene            complement(4146..>4358)
                     /locus_tag="AC16HB_06410"
     CDS             complement(4146..>4358)
                     /locus_tag="AC16HB_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606458.1"
                     /translation="VVLIAGYALHGGWDLLHEVQAHMQVDIFGGRAATRIPLAYGAFC
                     AMFDWAIAAYFVYRRRQWVAAWKSRA"
BASE COUNT          789 a         1397 c         1427 g          745 t
ORIGIN      
        1 gaggcgctga agccggcgct gcgcgcggtg ctggcgcgtg ccaacggggt ggacagcgcg
       61 cagtggatgg gcaccctgcc gcacaccgcc agcgcggcca cactcctcga cgagaccgac
      121 ctgaagcccg atgccgtggt cggcccgtat cggctgatcc gcgaaatcgg caccgggggc
      181 atgggcgcgg tgtggctggc ggagcgcgcc gacggcacgt tgaagcggca agtcgcgctg
      241 aagctgccgc gggcgatctg gtcgccggga ctggcgcaac ggatgcagcg cgagcgcgac
      301 atcctggccg cgctcgaaca cccgcacatc gcgcggctgt acgacgcggg caccgacgcg
      361 cagggccggc cgtttctcgc gctggagtac gtcgaaggtc agccgatcga tgcgtactgt
      421 cgcgcgcgga ccttgtccat caaggcgcgg ctgcagttgc tgctgcaggt ggcgcacgcg
      481 gtggcgttcg cgcacagccg gctggtgatt catcgggacc tgaagcccag caacatcctg
      541 gtgactgccg atggccaggt gcggctgctc gacttcggca tcgccaagct catggagggc
      601 gaacgcaccg ccgagacgca attgacgcag atcgccggca gagcgctgac gctggactat
      661 gcgagtccgg agcagatcaa gggcgagccg atcggcaccg ccagcgacgt gtacagcctg
      721 ggcgtggtgg cctacgagct gctggccgga gccaagccgt acaagctgaa gcgcggcagc
      781 gcggccgagc tggaggagac catcgccgcc gtcgacgcgc cgctggccag tgccaccgcc
      841 accaacaccc aaaccaggaa agaactgaag ggcgacctcg acgcgatcct gaacaaggcg
      901 ctgaagaaga accccgccga gcgctacgcc accgtcgatg ccttcgcgca ggacatcgag
      961 cgctacctgg ccggccaccg ggtcgtcgcc cgtcccgact cgctcgccta ccgactgacc
     1021 cgcttcgcca agcgctaccg cacgcctcta atcgcggcgg cgatcgccat cgccgccttc
     1081 ggcctggcca tcggcgtggg cgccaccgcg ctggtgatcc tcgccctgct gctcggactg
     1141 ggcgcggcac tgtggcaggc acgcaaggcg cgggagcaga ccagaatcgc gcagcagcag
     1201 agagacgcag cccgaaaaga ggcgcaccgc gctcaggcgg tgcagggctt cctcgtcaag
     1261 gtgttcgagc tgaactcaag cgctcaagcg gatccgatca gggcgcgcca gaccacggtg
     1321 cgtgaggtgc tcgacctcgg cgccgccgag atcccacaga cactgaagga cgcgcctgag
     1381 gcgcagcttg aagtaatgca tgtgctgagc agcatgtacc tggatcttgc gctcggtgac
     1441 caggccgttg ccctgatgca aagtggcgta caggtggcgc gccggacatt cggtccgtac
     1501 gggacacagc ttgcggaagc gctgctgtgg tacgcgcggg ggctgcacca ggaagcgcgg
     1561 cgcacagaga tcccgccttt gctcgaggaa gcgcgcgcca tcctcgatcg cgagggcgaa
     1621 tccacatcgt tcctgcgagc tgcgctgctc cttggaacgg cgcattatct tcgggacgag
     1681 tcgctctctg ccgcgcgccg aagtgctgat gggcttgtgg cgtttctccg cgcacatcat
     1741 ccggagcgac cagcattgat cgccgcgctc cgtcttgcag ttcgaacacg attgttcgcg
     1801 ttcgaacacg ccggcgccaa ggagcttgcg cagcaggccg tcggccaggc aagaggtctg
     1861 ggacccacaa gagcggcctg gctcgtcggc gcgcttggcg acctcggaga gactcaatgg
     1921 cttcaggctg aattcgatgc tgctgaaaac agctatcgcg aagcggtggc gacggcgctg
     1981 caagcaaacg gtcccgatca tcccaggacg ctgggagcgc gcgccacttt cgccgccctg
     2041 ttgctgaaca ccggtcgacg cgacgagggc gaatcaatcg cggccgatgt gctccagcgt
     2101 atcgagcagg ccgagttgaa gctggggcgt ggccacgcag tcgaaatccg cagcatcctc
     2161 gcggtcgccc gctgggaact cggctacccg gcggacgcag agggtgttat cgaggcgcac
     2221 gtggagaagc tgcgcaccac ccatccaaac tccggcactc tggcgttccg actgcgcaca
     2281 ctggcagaaa tcaagattgc acttggcctg catgatgagg cggagcgcct gcttcggcag
     2341 agcgaagccg tctggcatcg gtttgctgga ccggatcgtc cgcaggaggg ggaccgtcag
     2401 ttcgccatga cgcgcgtgcg ccttctcaat gcgaagggcg agcacggatc tgccttgggt
     2461 cagctccaaa caatgccaag gcttgacccg acggccccga ggctggacat gtcgctgctt
     2521 ttgatcgagt tggagcgcgc ggtcacggcg ctcgacatcg gaagagttga cgatggcgcc
     2581 gcgtccgctc gaaccgtcgt tcagctcatt cagcgctttg ccggcggacg gctgccactg
     2641 attgaagcac gcgcactcat gttgctggag cgcgccgctt tgatgacagg cggcatggca
     2701 gctgaacatg gcaatcgtca gtgcgcgcga tggacttgaa gttgacccga tttcgcggac
     2761 acctcatcct gatggaagaa ggccagcgcc tcgaaccgcc gctcactctg tgatccggcc
     2821 acggcccgcc ttgatctctg accgcttgga cttgccagcc agtctgcgga gccgagaggc
     2881 gtacgtcggc ttcgtcgccc tgcgcgcctt gggaacgtgt tcggcttgtt cgatcagttc
     2941 cgtcagccgc accagggcat ctgccttgtt gagttcttgg ctgcgcgcac tttgtgcctt
     3001 gatgacgact tcgccgtcct tcgtgattcg actgtcgttg agcgccagca ggcgggcctt
     3061 gactgcgagc ggcaggcccg aggtgtggac gttgaaccgc agttgcactg ccgtggcaac
     3121 cttgttgacg ttctgaccgc caggcccttg cgctcgcacc gcgctgaact caacttctcc
     3181 gtctgcgaag acatgccgca tcgtcgcgta gtgctaccta aggttcgagc tgagtcgacc
     3241 gcgaaggcag gcggcgtaag cccgggctga gacaatgtac ggagtacccc agaccgggct
     3301 tacgctgcct gcggtagcgg gtcggctcga gcgatggatt aggccgcact gagatgcacc
     3361 ttggacacct tagcgacgat ttctgttcgg cgagtcgcga gattaggcag gaatgtaggt
     3421 caacctgatt acatcctcgc caatccggtc atgagtcatg aggcggagcc gcggccgggg
     3481 tccggcgaaa tatggtttgc cgtgaccaag cacgacgggg tgcaggtaga ttcgatactc
     3541 atcgatcagg ccaagttcgg cgaggctgtg cgccaagttg gggccagcaa cttcgatctc
     3601 cccgtcgcgc tcggctttca tctcgcggat cgcgcgcgca agatcatcct caacaagcct
     3661 ggcgttgggg ccgaccgact tcaacgagcg cgagacgacc catttcggct ggctccgcca
     3721 cgccgccgcg aaggcatgtt cctctgcatc ccattcagga tgatcgtcgt cccagtaccg
     3781 catgacctca tacatcatgc gaccgtagac gctgcccgcc tgcctctgag cctcctcgat
     3841 gaagtggcgg aagagcgcgg ggcttggcgc aaacgccaga tggtcgacgt agccgtccag
     3901 ggactggttc attccgaaca cgagcttagc cataccactc cctttcctcg cgcggcacat
     3961 ccgcgcggtc ttgagcatcg ttgatggccg agacgttgcg agttgagcgg cctgacgtac
     4021 tggctcagcc gcggccttga cggccgaccg caggccgggc ggcagggacg tcggccggag
     4081 cgcagagttg ggcggctgcg agagaagttt tccgaagaaa gccttggctg ccccagcctc
     4141 catgatcacg ctcgtgattt ccaggcagcg acccattggc ggcgccgata gacaaaatat
     4201 gcggctatgg cccagtcgaa cattgcacag aatgcgccat aggcgagagg aattcttgtc
     4261 gctgctcttc ctccgaagat gtccacctgc atgtgggcct gaacctcatg caacaggtcc
     4321 cagcctccat gcagtgcgta tccagctatc agcacaac
//
LOCUS       DBNHBY010000262         5753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1030344, whole genome shotgun sequence.
ACCESSION   DBNHBY010000262 DBNHBY010000000
VERSION     DBNHBY010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1030344"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..883
                     /locus_tag="AC16HB_06415"
     CDS             89..883
                     /locus_tag="AC16HB_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5606459.1"
                     /translation="MAACTTTGVRQAPIVERAPPTAGATPEVPTTPPVKREARDGIYV
                     VQKGDTLRQIGAAFGHDPRDIARWNNIEDPNTLRPGQELRVVPPADAAVATPVTPATG
                     AQSRPLTTTVPGVAPSGDATTSPATAAPPAAAVAPSTAPPAGLTWAWPLTGKLLEKFD
                     EKRNKGIDIAAREGDPVLAAGDGSVVYSGSGLRGYGNLVIVKHNDEFISAYAHNRQIL
                     VKQGQAVKRGQRIAEAGKTDAPTPKLHFEIRRGGKPVDPILYLPPR"
     gene            889..1668
                     /locus_tag="AC16HB_06420"
     CDS             889..1668
                     /locus_tag="AC16HB_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5606460.1"
                     /translation="MRPTLVFDLETVPDVAGLRRLRTQCNDLSDADVAAQAFAERREK
                     TGSDFLPHHLHRVVAIGCAFRDEQGFRVRCLGAESDDEPKLIADFFRLIDRYTPQLVS
                     WNGGGFDLPVLHYRGLIHGVAAARYWEMGEDDRDFKWNNYISRYHTRHLDLMDLLALY
                     QPRANAPLDELARLAGFAGKLGMDGAKVWDAYAAGRLRDIRNYCETDVVNTYLVYCRF
                     QKMRGVLTAAEYGAELDLVRAALRDAGTPHWTEYLAAWPAH"
     gene            1671..3023
                     /gene="rlmD"
                     /locus_tag="AC16HB_06425"
     CDS             1671..3023
                     /gene="rlmD"
                     /locus_tag="AC16HB_06425"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR5606461.1"
                     /translation="MRERYTVEIDSLDLDGRGVARRDGKVLFVDGALPGELVEAETIR
                     AKPRYETARAVRIERESALRVVPRCRHFGLHAGACGGCSMQHLESRAQVALKQRALED
                     ALWHLGRVKPQNVLRPIVGPLWNYRHRARLTVRYVARKNGVLVGFHERASSYVAEMTT
                     CEVLPERVAALIVPLRELVNGLSLRERLPQIEVAVGAPADAQAIVLVLRVLQPLTDAD
                     VERLRAFGARHSVALWLQPGGPDSAAPLDAVDAIPLALPLPEFGVSIPFAPTDFTQVN
                     HRINEVLVRRAVRLLDAAPTDRVIDFFCGLGNFTLPLATRAACVLGIEGSAGLVRRAE
                     EAVRAHGLARAARFEVRNLFEWTDADWQRTVCSLGGADRALIDPPREGALAIARTLAA
                     AATRPSRIVYVSCNPPTLARDAAILVHEGGWAMRAAGVVNMFPHTSHVESVAVFDTRA
                     "
     gene            3151..3576
                     /gene="ndk"
                     /locus_tag="AC16HB_06430"
     CDS             3151..3576
                     /gene="ndk"
                     /locus_tag="AC16HB_06430"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR5606462.1"
                     /translation="MAVERTLSIIKPDAVAKNAIGQILARFEAARLQIVAARMMHLSR
                     GEAEAFYAVHRERPFFKDLVDFMVSGPIMVQVLEGENAITRNRELMGATDPKKAAKGT
                     IRADFADSIDANAVHGSDGPETARSEVAFFFPAMNIYAR"
     gene            3594..4703
                     /gene="rlmN"
                     /locus_tag="AC16HB_06435"
     CDS             3594..4703
                     /gene="rlmN"
                     /locus_tag="AC16HB_06435"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR5606463.1"
                     /translation="MDRVNLLGLDAAALTGFVDSLDEKPFRARQLSRWIHRRGVASFD
                     AMTDVAKSLRGKLAQCAEVVAPAVLRDSTSADGTRKWLLDVGQGNAIEAVFIPEDDRG
                     TLCISTQAGCAMNCLFCSTGRQGFNRNLTTAEIVGQLWLAEHSLRASGVTAEHERVIS
                     NVVLMGMGEPLQNLDAVLPALRLMLDDDAYGLSRRRVTVSTSGLVRQIDKLAAQCPVA
                     LAVSLHAPNDALRDELVPLNRKHPLRDLLAACRRYLRVAPRDFITFEYVMLDGLNDAD
                     AHAHELVNVVADVPCKFNLIPFNPFQGSGLARSSPERIRAFAQILTDAGIVTTIRKTR
                     GDDIDAACGQLAGEVRDRTRVAERRATRMPPVVAA"
     gene            4712..5515
                     /gene="pilW"
                     /locus_tag="AC16HB_06440"
     CDS             4712..5515
                     /gene="pilW"
                     /locus_tag="AC16HB_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HIR5606464.1"
                     /translation="MRKAFAAFVIGAGAWLAAACTTTTTVNGVPVPEARQSTPLPEAD
                     PRRRAEIRLQLAANYYQKGQLSVALEEVRRALSFDPGNALAHSMLGLIYLDLGDRREA
                     EASYAQALRLDPENPEILNNHGWFLCQTGRERESMEFFQRAAANRLYRTPGLAMQNAG
                     LCMLRVKDTAAAESFLRRSFELDAANPVTKFQLARLYLANRQFDRANFYYGLIDKGPN
                     PPPELLWLGLRIARGNADLRSERQLADDLRRRYPNSPEAAALSRGAFDE"
     gene            5508..>5753
                     /locus_tag="AC16HB_06445"
     CDS             5508..>5753
                     /locus_tag="AC16HB_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5606465.1"
                     /translation="MSETEAPAQRPADTGAIGPSFGRRLGAAREALALSVNDIAARLR
                     ISAKQVSALEREDLAALPGTAFVRGIVRNYAKEVRIDP"
BASE COUNT          953 a         1893 c         1971 g          936 t
ORIGIN      
        1 ggacgaaatg atcaggatga cgatacggat gatgggcaag ccgattcgaa agccgatttc
       61 ggtcctgttg ctggccggcg cattgctggt ggcggcctgc acgacgacgg gtgtgcgcca
      121 ggcgcctatc gtggagcgcg ctcctccgac cgcaggcgcg acgccagaag tccctacgac
      181 gccgcccgtt aagcgcgagg cgcgtgacgg catttacgtg gtgcagaagg gcgacacgct
      241 gcggcagatc ggcgccgcat tcggccatga cccacgcgac atcgcgcgct ggaacaacat
      301 cgaggacccg aacacgctgc gtcccggaca ggagctacgc gtcgtgccgc cggccgacgc
      361 ggccgtggcc acaccggtca cgcctgccac cggcgcgcag tcgcggcccc tgacgacgac
      421 cgttcccggc gtcgcgccat cgggagacgc aacgacgtcg cccgcaacgg ccgctccgcc
      481 cgcggcggcg gtagcgcctt caaccgcgcc accggcgggg ctgacctggg cttggccgct
      541 gaccggaaag ctgctcgaga aattcgacga gaagcgcaac aagggtatcg acatcgccgc
      601 gcgcgagggc gatccggtgc tggccgctgg cgacggcagc gtcgtctaca gtggcagcgg
      661 gctgcgtggc tatggcaacc tcgtgatcgt caagcacaac gacgagttca tctcggccta
      721 tgcgcacaat cgccagattc tcgtcaaaca ggggcaagcg gtaaaacgcg gacagcgcat
      781 tgccgaggct ggcaagaccg acgcgcccac gccgaagctg catttcgaga tccgtcgcgg
      841 cggcaagccg gtcgatccaa tcctgtacct gccgccgcgg tgacgcgcgt gaggccgacg
      901 ctcgtcttcg atctcgagac cgtgcccgat gtggcggggc tgcgtcggct gcggacgcag
      961 tgcaatgacc tgtccgatgc cgacgtcgcg gcgcaggcct tcgccgaacg gcgcgagaag
     1021 accggcagcg atttcctgcc gcatcacctg catcgcgtgg tggcgatcgg ctgcgccttt
     1081 cgcgatgagc agggattccg cgtgcgctgt ctgggcgcgg agagcgacga cgagccgaag
     1141 ctgatcgccg acttcttcag gctgatcgat cgctacacac cacagctggt gagttggaac
     1201 ggtggcggtt tcgacctgcc ggtgctgcac taccgcgggc tgatccacgg cgtcgccgct
     1261 gcgcgttact gggaaatggg cgaagacgat cgcgacttca agtggaacaa ctacatcagc
     1321 cgctaccaca cgcgtcatct cgacctgatg gacctgctgg cgctgtatca gccgcgcgcc
     1381 aacgcgccgc tggacgaact ggcgcggctc gccggattcg ccggcaagct cggcatggac
     1441 ggcgccaagg tgtgggacgc gtacgccgcc ggtcggctgc gtgacatccg caactactgc
     1501 gagacggatg tcgtcaacac ctacctggtc tactgtcgct tccagaagat gcgcggcgtg
     1561 ctgaccgcag ccgagtacgg cgccgaactc gacctggtgc gcgcggcgct gcgcgacgcc
     1621 ggcacgcccc actggaccga atacctcgcc gcctggccgg cgcattgacg gtgcgcgagc
     1681 gctatacggt cgagatcgac tcgctcgatc tggatgggcg cggggtggcg cgccgtgatg
     1741 gcaaggtact gtttgtcgac ggcgcgctgc ccggcgagct cgtcgaggcc gagacgatcc
     1801 gcgccaagcc tcggtatgag acggcgcgcg cggtccggat cgaacgcgag agcgcgctgc
     1861 gtgttgtacc gcgctgccgt catttcgggc tgcacgccgg cgcctgcggc ggctgctcga
     1921 tgcagcacct cgagtcgcgc gcgcaggtcg cgctcaagca gcgcgccctg gaagacgcac
     1981 tgtggcatct ggggcgggtg aagccgcaga acgtgctgcg gccaatcgtg ggtccgttgt
     2041 ggaactatcg ccaccgcgcg cgcctgaccg tgcgctacgt cgccagaaag aatggggtgc
     2101 tggtcggctt ccacgaacgc gccagcagct acgttgccga gatgacaacg tgcgaggtgc
     2161 tgcccgagcg cgtcgcggcg ctgatcgtgc cgctgcgcga actcgtgaat gggttgtcgc
     2221 tgcgcgagcg cttgccgcag atcgaagtcg cggtgggtgc accggcggac gcgcaggcga
     2281 tcgtgctggt gctgcgcgtg ctgcagccgc tgaccgatgc ggatgtcgaa cgactgcggg
     2341 cattcggcgc gcggcattct gtcgcgctat ggctgcagcc gggcgggccg gattcggcgg
     2401 caccgctgga cgcggtggac gcgatcccgc tcgcgctgcc gctgccggag ttcggcgtgt
     2461 cgattccgtt tgccccgacc gatttcacgc aggtcaatca ccggatcaac gaagtgctgg
     2521 tgcggcgtgc ggtgcggctg ctcgatgccg cgccgaccga ccgcgtgatc gacttcttct
     2581 gcgggctcgg caatttcacc ctgccgctgg cgacgcgcgc agcgtgcgtg ctcgggatcg
     2641 aaggcagcgc cggcctcgtg cggcgcgccg aagaagccgt gcgtgcccat gggctggcgc
     2701 gggctgcgcg gttcgaagtg cgtaaccttt tcgagtggac cgacgccgac tggcagcgca
     2761 ccgtgtgcag cctcggcggc gccgaccgcg cactgatcga tccgccgcgc gaaggcgctt
     2821 tggcgatcgc gcgcacgctg gcggcggcag ccacgcgtcc gagccgcatc gtctacgtgt
     2881 cgtgcaatcc gcccacgctc gcgcgtgacg ccgcgatcct cgtgcacgaa ggcggctggg
     2941 ccatgcgcgc agccggcgtc gtcaacatgt tcccgcacac atcgcatgtc gaatcggtgg
     3001 cggtgttcga cacgcgcgca tagcaaaaac ccgcgggaaa catggcggcc gtgctaagat
     3061 ttcgcgttca tcagtctccc taacgccttc atttatcgaa gcttttcgga aggcgcttca
     3121 accttctggc gcacgcacga gtgacacgaa atggccgttg aaagaaccct ctccatcatc
     3181 aaacccgacg cagttgccaa gaacgcgatc gggcagatcc tcgcgcgctt cgaagcggcc
     3241 agactgcaga tcgttgccgc gcgcatgatg catctgtcgc gcggcgaagc ggaagcgttc
     3301 tacgccgtgc atcgcgaacg gccgttcttc aaggatttgg tcgacttcat ggtgtctgga
     3361 ccgatcatgg tgcaagtgct cgaaggcgaa aacgccatca cacggaaccg cgaactgatg
     3421 ggcgccaccg acccgaagaa ggcggcgaag ggaacgatcc gtgccgactt cgccgacagc
     3481 atcgacgcca acgcggtgca cggttccgac ggtcccgaga ccgcgcgcag cgaagtcgcg
     3541 ttcttcttcc cggcgatgaa catctacgcc cgataggcgc agtcgacgtt tgcatggatc
     3601 gcgtcaacct tcttggactg gacgccgcgg cactgacggg tttcgtcgat agtctcgacg
     3661 agaagccgtt ccgcgcgcgc cagctgtcgc gctggatcca ccggcgcggc gtggcgagct
     3721 tcgacgcgat gacggatgtg gcgaaatcgc tgcgcggcaa gttggcgcag tgcgcggagg
     3781 tcgtggcgcc agcggtgctg cgcgattcga catccgccga cggcacccgc aaatggctgc
     3841 tcgacgtggg ccagggcaac gcgatcgagg cggtgttcat tccggaagac gaccgcggca
     3901 cgttgtgcat ctcgacgcag gccggctgcg cgatgaactg cctgttctgt tcgactggca
     3961 ggcagggctt caatcgcaat ctgaccacgg ccgagatcgt ggggcagctg tggctggccg
     4021 agcattcgct gcgcgcttcc ggcgtcacgg ccgagcacga gcgcgtcatc agcaacgtcg
     4081 tgctgatggg gatgggagag cccttgcaga atctcgacgc cgtccttccg gcgttgcgac
     4141 tgatgctcga cgacgacgcc tacggactgt cgcgccggcg cgtgacggtg tcgacctccg
     4201 gactggtgcg gcagatcgac aaactcgccg cgcagtgccc cgtcgcgctg gcggtgtcgc
     4261 tgcatgcacc gaacgacgcg ttgcgcgacg aactcgtgcc gctgaaccgc aagcacccgc
     4321 tacgcgacct gctggccgct tgccgccgct acctgcgcgt ggcgccgcgc gatttcatca
     4381 cgttcgagta cgtgatgctc gatggcctga acgatgccga cgcgcatgcg cacgaactgg
     4441 tcaacgtggt ggccgatgtg ccgtgcaaat tcaacctgat cccgttcaat cccttccagg
     4501 gctctggact ggcgcgctct tcgccggagc ggattcgtgc gttcgcgcag atcctgaccg
     4561 atgccggcat cgtgaccacg attcgcaaga cgcgcggcga tgacatcgat gccgcctgcg
     4621 gccagctcgc cggcgaggtg cgggatcgca cgcgcgtggc cgagcggcgc gcgacgcgca
     4681 tgccgccggt cgtcgcggcg tgagctcggt catgcgaaag gcgttcgctg ctttcgtcat
     4741 cggggccggt gcgtggctgg cggcggcatg cacgaccacg acgacggtca acggcgtgcc
     4801 ggtgcccgaa gcgcgccagt cgacgccgtt gcccgaggcc gatccgcgcc ggcgagccga
     4861 gatccggctg caactggccg ccaactatta ccagaagggc cagctgtcgg tcgcgctgga
     4921 ggaagtgcgg cgcgcgctgt cgttcgatcc gggcaacgcg cttgcgcact cgatgctcgg
     4981 cctgatctat ctcgatctgg gcgatcgtcg cgaggccgag gcaagctacg cacaggcgct
     5041 gcggctcgat ccggagaatc cggagatact gaacaaccac ggctggttcc tctgccagac
     5101 gggccgcgag cgtgaatcga tggaattctt ccagcgcgcg gccgcgaacc ggctgtaccg
     5161 cacgccggga ctggcgatgc agaacgcggg gctgtgcatg ctgcgcgtga aggacactgc
     5221 cgccgctgag agcttcctgc gccgttcctt cgagctcgac gccgccaatc cagtcaccaa
     5281 gtttcagctc gcacggctgt atctcgccaa ccgccaattc gaccgcgcga acttctacta
     5341 cggcctgatc gacaaagggc cgaatccgcc gcccgaactg ctgtggctgg ggctgcgcat
     5401 tgcgcggggg aatgccgacc tgaggtcgga gcgacaactc gcggatgatc tgcgccgtcg
     5461 ctatccgaac tcacccgagg ccgcggcgct gtctcgcggt gcgttcgatg agtgaaaccg
     5521 aagctcctgc gcaacgaccg gcggacaccg gggcgatcgg tccttccttt ggccggcgcc
     5581 tgggcgccgc gcgcgaggcg ctcgcactgt cggtgaacga cattgcagcc cggctgcgca
     5641 tcagcgcgaa gcaggtgagc gcgctcgagc gcgaagatct cgcggcattg ccggggacgg
     5701 cattcgtgcg cggcatcgtg cgcaactacg cgaaggaagt gcgcatcgat ccg
//
LOCUS       DBNHBY010000263         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1234059, whole genome shotgun sequence.
ACCESSION   DBNHBY010000263 DBNHBY010000000
VERSION     DBNHBY010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1234059"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2677)
                     /gene="carB"
                     /locus_tag="AC16HB_06450"
     CDS             complement(<1..2677)
                     /gene="carB"
                     /locus_tag="AC16HB_06450"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5606466.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADSIYIEPVNWRTVARIIEKERPDALLPTMGGQTALNCALDL
                     VREGVLEKFGVELIAASREAIDMAEDREQFRNAMRDIGLECPRSRVAHSMEEALAIQA
                     EIGFPCVIRPSFTLGGTGGGIAYNREEFEQIVARGLDASPTSEVLLEESVIGWKEFEM
                     EVVRDKADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRDASIAVLRKIG
                     VECGGSNVQFAVSPTDGRLLVIEMNPRVSRSSALASKATGFPIAKVAAKLAIGYTLDE
                     LRNDITGGATPASFEPTIDYVVTKIPRFTFEKFPGANARLTTQMKSVGEVMAMGRTFK
                     ESLQKALRGLETGIDGLTPVATLPLADDDARNALLEELRMPGPHRLRYVADAFRAGYS
                     FAEVQRASRIDSWFLEQVADLVREEAAVTAGGLAALDEPRLRALKRQGFADSRLAKLL
                     GVSEATVRDRRHALGVRPVYKRVDTCAAEFATSTAYMYSTYEEECEAAPTDRRKVVIL
                     GGGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTF
                     EDVMEIMHVERPYGVIVQYGGQTPLKLAKALGAAGVPIIGTTPDSIDIAEDRERFQQL
                     VDRLQLRQPPNRTARTEDEAVRLAAEVGFPLVVRPSYVLGGRAMEVVFSEDDLRVYMN
                     TAVQVSNDSPVLLDRFLDLAIEVDVDAICDGKDVYVGGIMEHIEQAGVHSGDSGCSLP
                     PNSLSPQLQDQIRDQTRRMALALNVVGLMNVQFAIQSGVVYVLEVNPRASRTAPFVSK
                     ATGVAMAKAGARVMTGRTLAQLGLVKERVPAYFSVK"
     gene            complement(2682..3848)
                     /gene="carA"
                     /locus_tag="AC16HB_06455"
     CDS             complement(2682..3848)
                     /gene="carA"
                     /locus_tag="AC16HB_06455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR5606467.1"
                     /translation="MTTPAVLALEDGTVFRGVSIGASGTATGEVVFNTAMTGYQEILT
                     DPSYARQIVTLTYPHIGNTGTNPEDLESNLVHAAGLVIRDLPALHSSWRATESLPQFL
                     QRRNTVAIAEIDTRKLTRILREKGAQAGCIASGDAAQRPDLAVEAARRFPGLKGMDLA
                     KVVCTQQPYQWNEGTNWSLEQGPKKRAGQRLHVVAYDFGIKRNILRLLADHGCRMTVV
                     PAQTSAREVMALGPDGVFLSNGPGDPEPCTYAIEATREFLAQGVPVFGICLGHQLLGL
                     AVGARTVKMKFGHHGANHPVLEIETGRVMITSQNHGFAVDETTLPANVKVTHKSLFDG
                     SLQGIACTDRPAFSFQGHPEASPGPRDLFPLFGQFVESMLQKGAAASLRKTVQG"
BASE COUNT          589 a         1341 c         1385 g          686 t
ORIGIN      
        1 ccttgaccga gaagtaggcc ggcacgcgct ccttcacgag gccgagctgc gccagcgtgc
       61 gccccgtcat gacgcgcgcg cccgccttgg ccatggccac tcccgtcgcc ttggagacga
      121 acggagcggt gcgtgacgcg cgcggattga cctccagcac gtagacgacg ccgctctgga
      181 tcgcgaactg cacgttcatc aggccgacga cgttcagtgc gagcgccatg cggcgcgtct
      241 ggtcgcggat ctggtcctgc agctgggggc tcaggctgtt gggcggcagc gagcagcccg
      301 agtcgccgga gtgcacgccg gcctgctcga tgtgctccat gatgccgccg acgtagacgt
      361 ccttgccgtc gcagatcgcg tcgacgtcca cctcgatggc gagatcgagg aagcggtcca
      421 gcagcaccgg gctgtcgttg gagacctgca cagccgtgtt catgtagacg cgcaggtcgt
      481 cctcgctgaa caccacttcc atggcacggc cgcccagcac gtagctcggc cggacgacca
      541 gcgggaaccc gacctccgcg gcgagccgga cggcttcgtc ctcggtacgc gcggtgcgat
      601 tcggcggttg cctgagctgc aggcgatcca cgagctgctg gaagcgctcg cggtcttcgg
      661 cgatgtcgat cgaatcgggg gtggtgccga tgataggtac gccagccgcg ccgagcgcct
      721 tggcgagctt gagcggcgtc tggccaccgt actgcacgat gacgccgtaa ggccgctcga
      781 cgtgcatgat ttccatgacg tcttcgaagg tgagcggctc gaagtacagg cggtcggacg
      841 tgtcgtagtc ggtcgacacc gtctccgggt tgcagttgac catgatggtc tcgaacccgt
      901 cttcgcgcag cgcgagcgcc gcgtgcacgc agcaatagtc gaattcgatg ccctgcccga
      961 tgcggttcgg gccgccgccg aggatgacga ccttgcggcg gtccgtcggc gccgcttcgc
     1021 attcttcctc gtaggtcgaa tacatgtagg ccgtcgacgt ggcgaactcg gccgcgcagg
     1081 tgtccacccg cttgtacacc gggcgcacgc cgagggcgtg cctgcggtcg cgcacggtgg
     1141 cctcgctgac ccccagcagc ttcgcgaggc ggctgtccgc gaagccctgg cgcttcagcg
     1201 cgcgcaggcg cggctcgtcc agcgcggcga gaccgccggc cgtcacggcc gcttcctcgc
     1261 ggaccaggtc tgccacctgc tcgaggaacc acgagtcgat gcgcgaggcg cgctgcacct
     1321 cggcgaagct gtaaccggcg cggaaggcgt cggcgacata gcgcagccgg tgcgggccgg
     1381 gcatgcgcag ctcctcgagc agcgcgttgc gcgcgtcgtc gtcggcgagc ggcagcgtcg
     1441 cgacgggcgt cagtccgtcg atgccggtct cgagcccgcg cagcgccttc tgcagcgact
     1501 ccttgaaggt gcgccccatg gccatcacct cgcccacgga cttcatctgc gtggtgagac
     1561 gcgcgttggc gcccgggaac ttctcgaacg tgaagcgcgg gatcttggtg acgacgtagt
     1621 cgatggtcgg ttcgaacgat gccggtgtcg cgccgccggt aatgtcgttg cgcagctcgt
     1681 cgagcgtgta accgatggcg agcttggccg ccaccttggc gatcgggaac ccggtggcct
     1741 tcgaggcgag cgccgacgac cgcgacacgc gggggttcat ctcgatgacc agcagccggc
     1801 cgtcggtcgg gctgaccgcg aactgcacgt tcgagccgcc gcactcgaca ccgatcttgc
     1861 gcagcaccgc gatcgaggcg tcgcgcatga tctggtattc cttgtccgtg agcgtctgcg
     1921 ccggcgccac ggtgatggaa tcgccggtgt gcacgcccat cgggtcgagg ttctcgatgg
     1981 agcagacgat gatgcagttg tccgccttgt cgcggacgac ctccatctcg aactccttcc
     2041 agccgatcac ggattcctcg agcagcacct cgctggtggg cgaagcgtcg aggccgcgcg
     2101 cgacgatctg ttcgaattcc tcgcggttgt aggcgatgcc gccgccggtg ccgcccagcg
     2161 tgaacgaggg gcggatcacg cacgggaagc cgatctcggc ctggatcgcg agcgcttcct
     2221 ccatgctgtg cgccacgcgc gagcgcgggc attcgagccc gatgtcgcgc atcgcgttgc
     2281 ggaactgctc gcggtcctcg gccatgtcga tggcttcgcg cgaggccgcg atcagctcga
     2341 cgccgaattt ttccagcacg ccttcgcgga ccaggtcgag cgcgcagttg agcgcggtct
     2401 ggccgcccat cgtcggcagc agagcgtccg gtcgttcctt ctcgatgatg cgcgcgacgg
     2461 tgcgccagtt gaccggctcg atgtagatcg agtcggccat ctccgggtcg gtcatgatcg
     2521 tggccgggtt cgagttcacc aggatgaccc ggtagccctc ctcgcgcagc gccttgcagg
     2581 cctgcgcgcc ggagtagtcg aactcgcaag cctggccgat gacgatcggg cccgcgccga
     2641 tgatcaggat ggacttgagg tcggtgcgct tcggcatgtc gctacccctg gaccgtcttg
     2701 cgcagcgagg ccgcggcgcc cttctgcagc atcgattcga cgaactgccc gaacagcggg
     2761 aacaggtcgc gcggtccggg gctcgcctcg gggtggccct ggaaactgaa cgcggggcgg
     2821 tcggtgcagg caatgccctg cagcgagccg tcgaacagcg acttgtgcgt caccttcacg
     2881 ttcgccggca gcgtcgtctc gtccacggca aagccgtgat tctgcgaggt gatcatcacc
     2941 cgcccggttt cgatctcgag caccgggtgg ttcgcgccgt ggtgcccgaa cttcatcttg
     3001 acggtgcgcg cgccgacggc gaggccgagc agctggtggc cgagacagat tccgaacacg
     3061 ggcacgccct gcgccaggaa ctcgcgcgtc gcctcgatcg cgtaggtgca cggctcgggg
     3121 tcgccggggc cgttcgacag gaacaccccg tcgggcccga gcgccatcac ctcgcgtgcc
     3181 gaggtctgcg ccggtaccac cgtcatgcgg caaccgtgat cggcgagcag gcgcaggatg
     3241 ttgcgcttga tgccgaagtc gtacgccacc acgtgcagcc gctgccccgc gcgcttcttc
     3301 ggcccctgct ccagcgacca gttggtgccc tcgttccact ggtacggctg ttgggtgcag
     3361 acgaccttgg cgaggtccat gcccttgagg cccgggaagc gacgcgccgc ctccaccgcg
     3421 aggtcggggc gctgcgctgc gtcgccgctg gcgatgcagc ccgcctgggc acccttctcg
     3481 cgcaggatgc gggtcagctt gcgggtatcg atctcggcga tcgcaacggt gttgcgccgc
     3541 tgcaggaact gcggcagcga ctcggtggca cgccagctgg agtgcagcgc cggcaggtca
     3601 cggatgacca ggcccgcggc gtgcacgagg ttcgattcga ggtcttccgg gttggtgccc
     3661 gtgttgccga tgtgcgggta cgtcagcgtg acgatctgtc gcgcgtacga cgggtcagtc
     3721 aggatttcct gatagcccgt catggccgtg ttgaacacga cctcgccggt ggcggtgccc
     3781 gatgcgccga tcgagacgcc ccgaaacacg gtgccgtcct cgagagccaa gacagcgggt
     3841 gtcgtcacgg gtacctccgg caagcgctct caaccctaca ttcagcgcgt gcgacgactg
     3901 ccggggttga gcggggctca ctccggtgca gttttgtctc acgggctaag tgaagaattg
     3961 tacgcgagcg tcccggcccg cgccacgcgc cgccgtcaga t
//
LOCUS       DBNHBY010000264        12721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_963092, whole genome shotgun sequence.
ACCESSION   DBNHBY010000264 DBNHBY010000000
VERSION     DBNHBY010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12721
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_963092"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="AC16HB_06460"
     CDS             complement(<1..665)
                     /locus_tag="AC16HB_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR5606468.1"
                     /translation="MYVRYFGLKQPPFSIAPDPRYLFMSERHREALAHLLYGVRGGGG
                     FVVLPGEIGAGKTTVCRCFLEQVPKRCNVAYIFNPKLTVEELLRSICDEYGIPYWHEG
                     PGVATVKDYVDALNEYLLRMHAVGHDNVLIIDEAQNLSAEVLEQLRLLTNLETNKRKL
                     LQIVLIGQPELRTMLARPEMQQLAQRVIARYHLGALSEADTVHYVRHRLEVAGLTRDL
                     PFDA"
     gene            complement(756..1223)
                     /locus_tag="AC16HB_06465"
     CDS             complement(756..1223)
                     /locus_tag="AC16HB_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR5606469.1"
                     /translation="MSEVSTKPYLIRAICDWCNDAGFTPYVAVAVDESVRVPMEFVKN
                     GEIVLNVSALATNRLKLGNDAIEFQARFSGVPREVYVPVVRVIAVYARENGQGMAFEV
                     PRGEAPAGVAPAQPDASTERGALVPVASVPDSGPEPTPPPPKPGDRPKLTRVK"
     gene            complement(1240..1842)
                     /locus_tag="AC16HB_06470"
     CDS             complement(1240..1842)
                     /locus_tag="AC16HB_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5606470.1"
                     /translation="MMVLYSGTTCPFSQRCRFVLFEKGMDFEIKDVDLFNKPEDINVM
                     NPYGQVPILVERDLILYESNIINEYIDERFPHPQLMPADPVMRARARLFLFNFERELF
                     IHVQTLENSNNQKAIDKGKLLIRDRLTQLAPILLKNKYMLGDDFSMLDVAIAPLLWRL
                     DHYGIDLPKSAAPLMKYAERIFSRPAYIEALTPSEKVMRR"
     gene            complement(1932..2795)
                     /locus_tag="AC16HB_06475"
     CDS             complement(1932..2795)
                     /locus_tag="AC16HB_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR5606471.1"
                     /translation="MKKLLASVTAAAALLFVAPPSAHATGGLAVPMDHWPEARAHDLA
                     ALQNGARLFANYCLTCHSASLMRWNRLQDIGLDDDQIKDFLIFGNQKVGDVMTVAMRP
                     ADSKLWFGKTPPDLSVITRARTSFEYKGTDYIYSLLRSFYRDASTPTGWNNAVFPSIG
                     MPHVLWERQGAREATIERVLHVEDEKTKKSSAVREVAVYDADGNVKVTRTPLPGHPDE
                     SIEFTFKAADPAAARQYDSDVADLVAYLAFMTDPSGAKRVRIGVWVLVFLGFLAVLTW
                     WLNREYWKDIK"
     gene            complement(2809..4206)
                     /locus_tag="AC16HB_06480"
     CDS             complement(2809..4206)
                     /locus_tag="AC16HB_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5606472.1"
                     /translation="MGLHKTEFKGPLGWIDERMPTLMAEYRKHMSQYYAPKNFNFWYI
                     FGGLAITVLMIQIITGIFLVMNYKPDAASAFASVEYIMREVPGGWFIRYMHSTGASFF
                     FIVVYLHMFRGLLYGSYRKPRELVWIFGCLIFLCLMGEAFFGYLLPWGQMSYWGAQVI
                     VNLFSAIPLIGPDLAIFIRGDYVIGDATLNRFFAFHVIAIPLVLLGLVVAHVLALHDV
                     GSNNPDGVEIKDTLNPATGRPLDGIPFHPYYTVHDIFWLSLFMIAFFAVLFLAPEMGG
                     YFLEWNNFIPADPFQGPPHIAPVWYFTPFYSILRATTEYFMYALAAFTVAYALLVFFS
                     ARNSTARAATLGIALVLVVFMVVPGLKLDPKFWGVVLMGVSVVIFFGLPWLDCSPVRS
                     IRYRPGWHLTLLLVFAVIFLILGAFGTMPPEDTRNVISQVLTLLYLAFFLLMPWWSRM
                     GAFKTVPARVTFAAH"
     gene            complement(4221..4823)
                     /gene="petA"
                     /locus_tag="AC16HB_06485"
     CDS             complement(4221..4823)
                     /gene="petA"
                     /locus_tag="AC16HB_06485"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR5606473.1"
                     /translation="MSFSDTNDAVDPSRRAALLTTTAVGAAGCVAMAAPFVASLAPSE
                     RAKAAGAPVTADISQLAPGEKMTVEWRGKPVWILRRTPEMLESLKRADKEVADPSSTR
                     TAFPTPPYARNEYRSIKPEYLVVIGICTHLGCNPIGPLAAGTAAPLGNEPGFLCPCHG
                     STFDLAGRVFKNKPAPDNLEIPPYRYLSDTVVMIGDDSKA"
     gene            5056..5478
                     /gene="mscL"
                     /locus_tag="AC16HB_06490"
     CDS             5056..5478
                     /gene="mscL"
                     /locus_tag="AC16HB_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR5606474.1"
                     /translation="MSFVSEFREFAVKGNVMDLAVGVIIGAAFGKIVDSVVKDVVMPI
                     VNFVAGGQIDFSNKYWVLSGNVPAGAALAEAQKAGTVLAWGSFATVVINFVILALIIF
                     WMVKAMNKVKREAPPAPPAAPPEDVVLLREIRDALKKK"
     gene            5545..6711
                     /locus_tag="AC16HB_06495"
     CDS             5545..6711
                     /locus_tag="AC16HB_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR5606475.1"
                     /translation="MLRRLWLLFAQATTVGLALLFIVATLRPDWLRRAPLPTATPVVR
                     DVSIQQVASPPTRAGAVNSYADAARRAMPAVVSVYTTKEIRRTPSMDDPLFERFFGDR
                     LRGQAERVSSLGSGVIASSEGYILTNHHVVQAADEISIALADGRRLDARLIGADPETD
                     LAVLRIDAPRLPVITFGRADALSVGDVVLAIGNPFDVGQTVTMGIVSALGRNNLGINR
                     FENFIQTDAAINQGNSGGALVDSSGNLVGINSAIYSRSGGSIGIGFAIPTTIVTQVMD
                     QLVKSGRVVRGYFGIEPVDITPELVDALKLARNEGVVVRGVQRNAPAGKAGMEPGDVM
                     VSINGIGVRDTPGMLNQIAQLAPGSVARVKVLRAGREVDLDVTVAERPVPQERK"
     gene            complement(6722..7384)
                     /locus_tag="AC16HB_06500"
     CDS             complement(6722..7384)
                     /locus_tag="AC16HB_06500"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606476.1"
                     /translation="MSLLRIAALALLLFANVAGAQRLQILPDDDVDLTRGWAAYQAAD
                     HAKALEHFRKAAQRNQRVAQFNLAVMLVNGEGTRADPKEGVQWLRKAADNGLARAQYS
                     LAVLYDRGEHLPKSPTEATTWYLKAAEQGWRDAQVSAATQFFLGRGAPQDYAQAAKWY
                     ERAAEQGDEGACYIIASMYENGDGVPKDLNRAVYWYVQAASFGDEVARIKAREVAARI
                     KR"
     gene            complement(7421..8194)
                     /gene="tatC"
                     /locus_tag="AC16HB_06505"
     CDS             complement(7421..8194)
                     /gene="tatC"
                     /locus_tag="AC16HB_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5606477.1"
                     /translation="MSSPAPESPDQEQSFLSHLIELRQRIVRAALAVVIVFLALTPFM
                     KQIFELLSEPMMMALPAGTKLLATGVITPFLVPLKVTLFTSFLLALPYVLYQAWAFVA
                     PGLYQHERRLALPVIMSSVAMFALGVAYCYFVVFGVVFKFIAGFAPTTVNVAPDIEAY
                     FNFVMTMFLAFGLAFELPIVVVLMVRFGIAGIDKLKQARPYVIVGAFIVAAIFTPPDV
                     LSQLLLAVPLCVLYELGLQIGRFVAPREAPEPTATESTP"
     gene            complement(8203..8715)
                     /gene="tatB"
                     /locus_tag="AC16HB_06510"
     CDS             complement(8203..8715)
                     /gene="tatB"
                     /locus_tag="AC16HB_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR5606478.1"
                     /translation="MFDFSFGELAVISAVALVVLGPERLPKVARTVGDWVGKAQRYVS
                     QVKADINREMELAELKKLQEEARNAAQSLQSAVQDVQSGFRNVASSIETPATPADAPA
                     STDSAWEGAAESWQTLTFPKRYKPGPSVDELAEEVARLKRELARPDAHAGTRRKYAPR
                     GRVNRPRIRR"
     gene            complement(8797..9027)
                     /gene="tatA"
                     /locus_tag="AC16HB_06515"
     CDS             complement(8797..9027)
                     /gene="tatA"
                     /locus_tag="AC16HB_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR5606479.1"
                     /translation="MGSFSIWHWLIVLLVIVLIFGTSKLKNIGKDLGGAIKGFKEGVA
                     EGSSDPAKPAAKVEEAKQVAGQTIDVQAKEKS"
     gene            complement(9037..9384)
                     /locus_tag="AC16HB_06520"
     CDS             complement(9037..9384)
                     /locus_tag="AC16HB_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5606480.1"
                     /translation="MTYDPNCIFCRIVKGELPAKRIHEDDEFIVFPDIHPAAPVHLLM
                     VPKEHFATLADAQAKHAALLGRMLLLAPRLAREQGATNGYRVVVNNGPDGGQEVYHLH
                     VHVLGGARPWKRL"
     gene            complement(9399..9734)
                     /locus_tag="AC16HB_06525"
     CDS             complement(9399..9734)
                     /locus_tag="AC16HB_06525"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR5606481.1"
                     /translation="MNDETLRRLADVIESRKGGDPDRSYVARLLAQAPDAILKKIGEE
                     ATETVMAAKDGDAERIVAETADLWFHCLVMLAHHGLRPEQVLAELDRRAGTSGLEEKA
                     ARKARNRGH"
     gene            complement(9731..10114)
                     /gene="hisI"
                     /locus_tag="AC16HB_06530"
     CDS             complement(9731..10114)
                     /gene="hisI"
                     /locus_tag="AC16HB_06530"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR5606482.1"
                     /translation="MNWLDDIAFDERGLVTVIAQDVATHRVLMVAWADREALAETAAT
                     GQAVYFSRSRGRLWRKGEESGHRQQVREVRLDCDGDVVLYRVEPAGGIACHTGRESCF
                     FRRLDGTEWRTVDPVLKDPELIYRK"
     gene            complement(10114..10665)
                     /gene="lepB"
                     /locus_tag="AC16HB_06535"
     CDS             complement(10114..10665)
                     /gene="lepB"
                     /locus_tag="AC16HB_06535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5606483.1"
                     /translation="MSRLARDNVGLLLFLLGMFVFRSAVADWNSIPSGSMEPTLQVGD
                     RVLVDQHAYAWRLPFTRVHIGERGTPRAGDIVTFASPVDGERLIKRVVAVGGDTVAAR
                     RGVLYVSATPVGAAGRIEFGPLAVPAGHVFVMGDNHDDSFDSRFWGPLAVDRIYGRAI
                     KVVASFDGVRPRGDRFWIDLGRR"
     gene            complement(10674..11450)
                     /gene="hisF"
                     /locus_tag="AC16HB_06540"
     CDS             complement(10674..11450)
                     /gene="hisF"
                     /locus_tag="AC16HB_06540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR5606484.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGVNFVALRDAGDPVEIARRYDEQGA
                     DELTFLDITASSDQRDIILHVIEAVAEQVFIPLTVGGGVRKVDDVRRLLNSGADKISV
                     NTAAVQDPRLVAAATARFGSQCIVVAIDARRRQPHDPSRGWEVFTHGGRTPTGLDAID
                     WARRVAELGAGEILLTSMDRDGTKMGFDLELTRAVADAVPIPVIASGGVGTLQHLADG
                     IAEGGADAVLAASIFHYGEFTVRQAKEYLAAQGIPMRLEP"
     gene            12030..>12721
                     /locus_tag="AC16HB_06545"
     CDS             12030..>12721
                     /locus_tag="AC16HB_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5606485.1"
                     /translation="MRAPYWVFENLVVRGACATHDDCEHAFHVVGAAHDIVIRNNLLR
                     DFNAHIKINGDGERFPDAGQIVSNTLINGRPRQTDAPVTPIDLVAASGWTVEANLIAD
                     FVKAGGDQTSYGGFAKGGGAGNRFVRNVVLCEHRLRGAPGRRIGLSFGGGGSGKASCR
                     DRRCTVEHDGGEISGNLVMSCSDEGIYLNRAARARLMHNTVIDTAGVVVRYPDSSARA
                     DGNLIDGALRTR"
BASE COUNT         2182 a         4237 c         4204 g         2098 t
ORIGIN      
        1 gcgtcgaacg gcaggtcccg cgtcagcccg gcgacctcga ggcggtggcg cacgtagtgc
       61 acggtgtcgg cttcggacag cgcccccagg tgatagcgcg cgatcacccg ctgcgcgagt
      121 tgctgcatct cggggcgggc cagcatcgtg cgcaattcgg gctggccgat cagcacgatc
      181 tgcagcaatt tgcgtttgtt ggtctccagg ttcgtgagca gtcgcaactg ctcgaggacc
      241 tcggccgaca ggttctgcgc ctcgtcgatg atcagcacgt tgtcgtgccc gaccgcgtgc
      301 atccgcaaca gatactcgtt gagcgcgtcg acgtagtcct tgaccgtcgc gacccccggc
      361 ccctcgtgcc agtacggaat gccgtactcg tcgcagatcg agcgcagcag ttcttcgacc
      421 gtgagcttcg gattgaagat gtaggcaacg ttgcagcgct tcgggacctg ctcgagaaag
      481 cagcggcaca cagtcgtctt gccggcgccg atctcgccgg gcagcacgac gaacccgccg
      541 cccccgcgca cgccgtacag cagatgcgcg agcgcctcgc gatggcgctc gctcatgaac
      601 aggtaacgcg gatccggcgc gatcgagaag ggcggctgct tcagcccgaa atagcgcaca
      661 tacatgatgg acaggatacc tccccgccgc aggtcgagcc gcgcccgttg gttcgagcga
      721 gtgcagcaat gccggcgccg cgcgtcggca cccgcctact tgacgcgcgt gagcttcggc
      781 cggtcgcctg gcttcggcgg cggcggcgtg ggttcgggcc ccgagtcggg caccgaggcg
      841 acgggcacca gtgccccgcg ctcggtcgag gcatcgggct gcgccggcgc gactcccgcg
      901 ggcgcctcgc cgcgcggcac ctcgaaggcc atgccttgtc cgttctcgcg cgcatagacc
      961 gcgatcacgc gcaccaccgg cacgtacacc tcgcgcggca cgccggagaa gcgcgcctgg
     1021 aactcgatcg cgtcgttgcc gagcttcagt cgattggtcg ccagcgccga gacgttgagc
     1081 acgatctcgc cgttcttgac gaactccatc ggcacgcgca ccgactcgtc gaccgccacc
     1141 gcgacgtacg gcgtgaagcc cgcgtcgttg caccagtcgc agatggcgcg gatcaggtag
     1201 ggcttggtcg agacttcgct catgcggccg attgtgcgcc tatcggcgca tcactttttc
     1261 gctcggcgtc aacgcctcga tgtacgcggg gcggctgaag atgcgctcgg cgtacttcat
     1321 cagcggggcc gcgctcttcg gcaggtcgat tccgtagtgg tcgagtcgcc acagcagcgg
     1381 cgcgatcgcc acgtcgagca tcgagaagtc gtcgcccagc atgtacttgt tcttcagcag
     1441 gatcggcgcg agctgcgtca gacggtcgcg gatcagcagc ttgcccttgt cgatcgcctt
     1501 ctggttgttg ctgttctcca gcgtctgcac gtggatgaac agctcccgct cgaagttgaa
     1561 caggaacagg cgcgcccgcg cgcgcatcac cgggtcggcc ggcatcagct gcggatgcgg
     1621 aaagcgctcg tcgatgtact cgttgatgat gttcgactcg tacaggatca ggtcgcgctc
     1681 gaccaggatc ggcacctgcc cgtagggatt catcacgttg atgtcctcgg gcttgttgaa
     1741 gaggtcgacg tccttgatct cgaagtccat gcccttctcg aacagcacga aacggcagcg
     1801 ttgcgaaaac gggcaggtcg ttccggagta aagcaccatc atcttgtgtc ccctgaaaac
     1861 gattcagggt gaggctgccg cctcaccccg cagtcaacga cggagccgcc cgcggcggcc
     1921 gccccgatcg gctatttgat gtccttccag tactcgcgat tcagccacca ggtcagcacg
     1981 gcgagaaagc ccaggaacac cagcacccag acgccgatgc gcacgcgctt ggcgccgctg
     2041 ggatcggtca tgaacgccag ataggccacc aggtcggcca cgtcggagtc gtactggcgc
     2101 gccgcggccg gatcggctgc cttgaacgtg aactcgatcg actcgtcggg atggcccggc
     2161 agcggcgtgc gcgtgacctt cacgttgccg tcggcgtcgt aaacggcgac ctcgcgcacc
     2221 gcggacgact tcttcgtttt ctcgtcctcg acgtgcagca cgcgttcgat cgtcgcctcg
     2281 cgcgccccct ggcgctccca cagcacgtgc ggcattccga tgctggggaa cacggcgttg
     2341 ttccatccgg tcggcgtcga cgcgtcgcga tagaacgagc gcagcagcga atagatgtag
     2401 tcggtgccct tgtattcgaa cgacgtgcgc gcacgcgtga tcaccgacag gtcgggcggc
     2461 gtcttgccga accatagctt gctgtcggcc gggcgcatcg ccacggtcat cacgtcgccg
     2521 accttctggt tgccgaagat caggaaatcc ttgatctggt cgtcgtcgag accgatgtcc
     2581 tgcagccggt tccagcgcat cagacttgcg ctgtggcagg taagacagta gttcgcgaac
     2641 agccgcgcgc cattctgcag cgcagccagg tcgtgcgcgc gcgcctcggg ccagtggtcc
     2701 atgggcacgg cgagcccgcc cgtggcatgg gccgatggcg gcgccacgaa cagcagcgcg
     2761 gcggccgcgg tgacggatgc aagcagcttc ttcatgttcg cgtccccgtc agtgcgcggc
     2821 aaacgtgacg cgggccggca cggtcttgaa cgcgcccatc cggctccacc acggcatcag
     2881 caggaagaac gcgagataca gcagcgtcag cacctgagag atcacgttgc gcgtgtcctc
     2941 gggcggcatc gtgccgaacg cgccgaggat gaggaagatc acggcgaaca ccagcagcag
     3001 cgtcaggtgc cagcccggcc gatagcggat cgagcgcacc ggcgagcagt cgagccacgg
     3061 caggccgaaa aagatcacga ccgacacgcc catcagcacg acgccccaga acttcgggtc
     3121 gagcttcagc cccggcacga ccatgaaaac gaccagcacc agcgcgatcc cgagcgtggc
     3181 ggcgcgcgcc gtcgagttcc tggccgagaa gaacaccagc agcgcgtagg cgaccgtgaa
     3241 tgcggccagc gcatacatga agtactccgt ggtcgcgcgc aggatcgagt agaacggggt
     3301 gaagtaccag accggcgcga tgtgcggcgg accctggaac ggatcggccg ggatgaagtt
     3361 gttccactcg aggaagtagc cgcccatttc gggcgcaagg aacagcaccg cgaagaacgc
     3421 gatcatgaac agcgacagcc agaagatgtc gtgcaccgtg tagtaaggat ggaacggtat
     3481 gccgtccaac ggcctgccgg tcgcaggatt cagcgtgtcc ttgatctcca cgccgtccgg
     3541 gttgttcgag cccacgtcgt gcagcgcgag cacgtgcgcc accacgaggc cgagcagcac
     3601 cagcgggatc gcgatcacgt ggaacgcgaa gaagcggttc agcgtggcgt cgccgatcac
     3661 gtagtcgccg cggatgaaga tcgccaggtc gggaccgatc agcggaatcg ccgagaacag
     3721 gttgacgatc acctgcgcgc cccagtagct catctgcccc cacggcagca ggtaaccgaa
     3781 gaacgcctcg cccatcaggc acaggaagat caggcaaccg aagatccaca ccagttcgcg
     3841 cggcttgcgg taactgccat agagcagccc gcggaacatg tgcagataga cgacgatgaa
     3901 gaagaacgac gccccggtgg agtgcatgta gcggatgaac cagccgcccg gcacttcgcg
     3961 catgatgtat tcgaccgacg cgaacgcgct cgcggcatcc ggcttgtagt tcatcaccag
     4021 gaagatgccg gtgatgatct ggatcatcag caccgtgatc gcgaggccac cgaagatgta
     4081 ccagaagttg aagtttttcg gcgcgtagta ctgcgacatg tgcttgcggt attccgccat
     4141 caacgtcggc atgcgctcgt cgatccagcc gagcgggccc ttgaattcgg tcttgtgcag
     4201 tcccatggtg cggtctcgga tcaggccttg gagtcgtcgc cgatcatgac gacagtgtcg
     4261 gaaaggtacc tgtacggcgg aatctcgagg ttgtcgggcg ccggcttgtt cttgaacaca
     4321 cgccccgcca gatcgaacgt cgagccgtgg caggggcaga gaaagccggg ttcgttgccg
     4381 agcggcgccg ccgtccccgc cgcgagcggg ccgatcggat tgcagcccag gtgcgtgcag
     4441 atgccgatca cgaccagata ctcgggcttg atcgagcggt attcattgcg cgcgtagggc
     4501 ggcgtcggaa acgcggtgcg cgtcgaactc gggtcggcga cctccttgtc ggcgcgcttc
     4561 aacgactcga gcatctcggg cgtgcggcgc agtatccaaa ccggcttgcc gcgccactcg
     4621 accgtcatct tttcgccggg ggcaagctgc gagatgtccg cagtcaccgg tgcgccggct
     4681 gccttggcgc gctccgaagg cgccagcgaa gccacgaaag gggcggccat ggcaacgcag
     4741 ccggcggcgc cgaccgcagt ggtcgtcagc agagcggcgc gacgactggg gtcgacggcg
     4801 tcgttcgtat cagaaaagga catgggatct gtttgtggaa ggacgagaaa cgcgggcgtc
     4861 gcccgcgtgc agccggcaag tttaccgcat ggccgcttca cgcccctgtg ggaccagggc
     4921 gcgttgcata gccggcagcg cgtctcaatc accgtctgac gtgatggggg tcaacgcggc
     4981 ggatcggctt ggcccgggat aatggctcgg ccgccccccc ctggacgcgg cgacggagaa
     5041 agaaggagag gaacaatgag cttcgtgtcg gagttccgcg aattcgcggt caagggcaat
     5101 gtgatggacc ttgcggtcgg cgtgatcatc ggcgcggcgt tcggcaagat cgtcgattcg
     5161 gtagtcaagg atgtcgtcat gccgatcgtg aacttcgttg ccggcggaca gatcgatttt
     5221 tccaacaagt actgggtgtt gtcgggcaac gtaccggccg gcgcggcgct ggccgaagcg
     5281 cagaaagccg ggacggtgct cgcatggggc agtttcgcca ccgtggtgat caacttcgtc
     5341 atcctggcgt tgatcatctt ctggatggtc aaggcgatga acaaggtgaa gcgcgaggcg
     5401 ccgcctgcgc caccggccgc gccgccggag gacgtggtac tgctgcggga gatccgcgac
     5461 gctttgaaga agaagtagcg ggcccggcag cgatgctcgg cgccgataca atcgacgcct
     5521 tcagcatcaa aggccgttcc cgtcatgctc cgccgtctct ggctgctgtt cgcgcaagcg
     5581 accaccgtcg gcctcgcgct gctgttcatc gtcgccacgc ttaggccgga ttggctgcgg
     5641 cgcgcgccgc tgccgactgc cacgccggtg gtgcgcgacg tgtcgatcca gcaggtggcc
     5701 agcccgccga cacgcgcggg cgccgtgaat tcctacgcgg acgctgcgcg gcgcgcgatg
     5761 ccggccgtgg tcagcgtcta cacgaccaag gagatccgcc gcaccccgtc gatggacgat
     5821 ccgctgttcg agcggttctt cggcgaccgg ctgcgcggcc aggccgagcg cgtctccagc
     5881 ctgggctcgg gcgtgatcgc gtcgtccgag ggctacatcc tgaccaatca ccacgtcgtg
     5941 caggcggccg acgagatctc gatcgcgctc gccgacggcc gtcggctgga cgcgcggctg
     6001 atcggcgccg atccggagac ggatctcgcg gtgctgcgca tcgacgcgcc ccgattgccg
     6061 gtgatcacgt tcggccgggc ggacgcattg tcggtgggcg atgtggtgct ggcgatcggc
     6121 aacccgttcg acgtcggcca gaccgtcacg atgggcatcg tatcggcgct cgggcgcaac
     6181 aacctcggca tcaaccggtt cgagaacttc attcagaccg atgcggcgat caaccagggc
     6241 aactccggcg gcgcgctcgt cgacagcagc ggcaacctgg tcggcatcaa ctcggcgatc
     6301 tattcgcgct ccggcggatc gatcggcatc ggcttcgcga tcccgacgac gattgtcacg
     6361 caggtgatgg atcagctggt caagtcgggc cgggtcgtgc gcggctattt cggcatcgaa
     6421 ccggtcgaca tcacgcccga actcgtcgac gcgctgaagc tggcgcgcaa cgagggtgtc
     6481 gtggtgcgtg gcgtgcagcg caatgcgccg gcgggcaagg ccggcatgga gcccggcgac
     6541 gtgatggtgt cgatcaacgg catcggcgtg cgcgacacgc ccggcatgct gaaccagatc
     6601 gcgcagctgg cgcccggcag cgtggccagg gtgaaagtgc tgcgcgcagg ccgcgaggtc
     6661 gatctcgacg tgaccgttgc cgagcggccg gttccgcagg agcggaagta gctggggccc
     6721 gttaacgctt gatgcgcgcc gccacctcgc gcgccttgat ccgcgcgacc tcgtctccaa
     6781 acgacgcggc ctgaacgtac cagtagacgg cgcggttcag gtccttcggc acgccgtcgc
     6841 cgttctcgta catgctggcg atgatgtagc aggcgccttc gtcgccctgc tcggccgccc
     6901 gttcgtacca cttggccgcc tgcgcgtagt cctgcggcgc gccgcgaccc aggaagaact
     6961 gcgtggccgc gctgacctgc gcgtcgcgcc agccctgctc ggccgccttc agataccagg
     7021 tggtggcctc ggtcggcgac ttgggaaggt gctcgccgcg gtcatacagc accgccagcg
     7081 aatactgcgc gcgcgccagg ccgttgtccg ccgccttgcg cagccactgc acgccctcct
     7141 tcggatcggc gcgcgtgccc tcgccgttga ccagcatcac cgcgaggttg aactgggcga
     7201 cgcgctgatt gcgctgcgcg gccttgcgga agtgctcgag cgccttcgcg tggtcggccg
     7261 cctgatacgc ggcccagccg cgcgtcagat ccacgtcgtc gtccgggagg atctgcagcc
     7321 gctgcgcgcc ggcgacgttg gcgaagagca gcagcgcgag ggctgcgatg cgaaggaggc
     7381 tcatctgcgc ttggtcggtc gacggcgctg tttctgacgt tcagggcgtg gattcggtgg
     7441 cagtcggctc gggtgcttcg cgcggcgcca cgaagcgccc gatctgcagt cccagttcgt
     7501 atagcacgca cagcggcacc gcgagcagca actgagacag cacgtccggg ggcgtgaaga
     7561 tcgcggcgac gatgaatgcg ccgacgatca cgtacggccg cgcctgcttg agcttgtcga
     7621 tgccggcgat gccgaagcgc accatcagca cgaccacgat cggcagctcg aacgccagac
     7681 cgaacgcgag gaacatcgtc atgacgaagt tgaaataggc ctcgatgtcc ggtgcgacgt
     7741 tgaccgtcgt cggtgcgaag ccggcgatga acttgaacac cacgccgaag acgacgaagt
     7801 agcagtacgc cacgccgagc gcgaacatcg ccacgctcga catgatcacc ggcagcgcca
     7861 agcgccgctc gtgctgatac agcccgggcg caacgaacgc ccacgcctgg tacagcacgt
     7921 acggcagcgc gagcaggaac gacgtgaaga gcgtcacctt caacggcacc aggaacggcg
     7981 tgatcacgcc ggtggcaagc agcttggtgc cggccggtag cgccatcatc atcggctccg
     8041 acagcagttc gaatatctgc ttcatgaagg gcgtcagcgc caggaacacg atcacgaccg
     8101 cgagcgccgc ccgcacgatg cgctggcgca actcgatcag gtgcgacaga aagctctgtt
     8161 cctggtcggg cgactcgggt gccggcgagc tcattggcgc agtcagcgac gaatgcgcgg
     8221 ccggttcacg cgcccacgcg gcgcatactt acgccgcgtg cccgcatggg cgtcgggccg
     8281 cgccagttcg cgcttcaacc gcgcgacctc ttccgccagc tcgtcgaccg acgggccggg
     8341 cttgtagcgc ttgggaaaag tcagggtctg ccagctttcg gcagcgccct cccacgcgga
     8401 atcggtggac gcaggtgcgt cggccggagt ggcaggcgtc tcgatgctgc ttgccacgtt
     8461 cctgaacccg gactgcacgt cctgcacggc cgactgcagc gactgtgcgg cgttgcgggc
     8521 ttcttcctgc agtttcttca gttcggccag ttccatctcg cgattgatgt cggccttgac
     8581 ctggctgacg tagcgctgcg cctttcccac ccagtcgccg accgtgcggg cgaccttggg
     8641 caggcgctcg gggccgagca cgaccagcgc aaccgcgctg attacggcga gttcaccgaa
     8701 actgaaatcg aacatgggcg tcgggcgctc ggcgtcggac gcccgacgtc aggcgacttc
     8761 gcgcccgacg cctgcacccg acgcccgacg ggaagctcag gacttctctt tcgcctgcac
     8821 gtcaatggtc tggccggcga cctgcttggc ttcctcgacc ttggcggccg gcttggcggg
     8881 atcgctgctg ccctcggcaa cgccttcctt gaagcccttg atcgcgccgc cgagatcctt
     8941 gccgatgttc ttcagcttgc tggtaccaaa gatcagcacg atcacgagca gaacgatcag
     9001 ccaatgccaa atcgagaacg aacccattgt cagctcctaa agacgtttcc acggccgggc
     9061 gccgcccagc acgtgcacgt gcaagtggta gacctcttgc ccgccgtccg ggccgttatt
     9121 gacgacgaca cgatatccgt tcgtggcgcc ctgttctcgt gctagccgcg gtgcgagaag
     9181 cagcattcta cccaacagcg ccgcgtgttt cgcctgcgcg tcggcaagcg tcgcgaagtg
     9241 ctccttcggc accatcagca ggtgcaccgg cgcggccggg tgaatgtcgg ggaacacgat
     9301 gaactcgtcg tcctcgtgga ttcgcttggc ggggagttcc cccttgacga tgcgacagaa
     9361 gatgcagttg gggtcgtagg tcataggcgt tttgggagtc agtggccgcg gttgcgcgcc
     9421 ttgcgcgccg ccttctcctc caggcccgag gttccggcgc ggcggtcgag ttcggccagc
     9481 acctgctcgg gtcgaagacc atgatgcgcg agcatgacga ggcagtggaa ccacaggtcg
     9541 gcagtttcgg cgacgatgcg ctcggcgtcg ccgtccttgg ccgccattac ggtttcggtc
     9601 gcctcctcgc cgatcttctt caggatcgcg tcgggggcct gcgccagcag gcgcgcgacg
     9661 taggatcggt ccgggtcgcc gcccttgcgt gactcgatca cgtcggccag acggcgcagc
     9721 gtttcgtcgt tcatttgcgg tagatgagtt cggggtcctt cagcaccggg tcgaccgtcc
     9781 gccattccgt tccgtcgagc cggcggaaga agcaactctc gcgaccagta tggcaggcga
     9841 tgccacccgc cggttcgacg cgatagagca cgacatcgcc atcgcagtcg agccgcactt
     9901 cgcggacctg ctgccggtgg ccggactcct cgcccttgcg ccacagcctg ccgcgcgaac
     9961 gcgaaaagta gactgcttgt ccggtcgcgg ccgtttcggc aagggcctca cgatcggccc
    10021 acgcgaccat cagcacgcga tgggtcgcca catcctgggc gatcacggtc acgaggccgc
    10081 gctcgtcgaa cgcaatgtcg tcgagccagt tcatcagcgc ctgccgaggt cgatccagaa
    10141 gcggtcgccg cgcggccgca cgccgtcgaa cgatgccacg accttgatcg cgcgcccata
    10201 gattcggtcg actgccagcg gtccccagaa gcgcgaatcg aagctgtcgt cgtggttgtc
    10261 ccccatcacg aacacgtggc cggcgggaac tgcgagcggc ccgaactcga tcctgccagc
    10321 cgccccgacc ggcgtcgcgc tgacgtacag gacgccacgg cgcgcggcga ccgtgtctcc
    10381 gccgaccgcg acgacgcgct tgatcagacg ctcaccatcg acgggcgagg caaacgtcac
    10441 gatgtcgccg gcgcgcggcg ttccgcgctc cccgatatgg acacgcgtga atggcaatcg
    10501 ccatgcgtac gcgtgctgat cgacgagcac ccgatcgccg acctgcagcg tcggttccat
    10561 cgaaccgctg ggaatggagt tccagtccgc caccgcggag cggaacacga acatgcccag
    10621 cagaaacagc aggaggccga cgttgtctcg tgccagcctc gacattcgat tcatcatggc
    10681 tcgagcctca tcgggatgcc ctgcgcggcc aggtactcct tggcttgacg cacggtgaac
    10741 tcaccgtagt ggaagatcga cgccgccagc accgcgtcgg ccccgccctc ggcaatgccg
    10801 tccgccaggt gttgcagcgt gcccacgccg cccgaggcga tcaccgggat cggcaccgcg
    10861 tccgcgaccg cacgcgtcaa ttcgaggtcg aatcccatct tcgtgccgtc acggtccatg
    10921 ctggtcagca ggatttcgcc ggcaccgagt tcagccacgc ggcgcgccca gtcgatcgcg
    10981 tcgagcccgg tcggcgtgcg accaccgtgc gtgaaaacct cccagccgcg cgacgggtcg
    11041 tgcggctgcc tgcgccgcgc atcgatcgcc accacgatgc actgcgagcc aaagcgcgcg
    11101 gtcgcggcgg cgacgagccg ggggtcctgc acggcagcgg tgttgacgga gatcttgtcg
    11161 gcgcccgaat tcagcagtcg gcgcacgtcg tcgaccttcc gcaccccacc accgacggtc
    11221 agcgggatga acacctgctc ggcgaccgct tcgatcacgt gcaggatgat gtcgcgctgg
    11281 tccgaactgg cggtgatgtc gaggaacgtc agttcgtcgg caccctgctc gtcgtagcgg
    11341 cgcgcgatct cgaccggatc gcccgcatcg cgcagcgcga cgaagttcac gcccttgacc
    11401 acacgtccgg cggtcacgtc gaggcaggga ataatgcgtt tcgcgagcat gggagggaca
    11461 ctctgaagcc aagcatagtg caaccgcacc gcccacccca gagatcggac tcgcgggaag
    11521 cgcgccgccc gcagcgtcgt cgggccatcg ttgtcgcact ggtggcagcc gtcttatgcg
    11581 cgttcgccgc cgcggtggtc gcgatcgatt cgctcggcat ttcgccgcga ctgttggcgc
    11641 cctatctgga gcggcgcgcg agcggccaca atgcactgat cgaaaccgcc gcgagcatcg
    11701 tcagccggtg gctgatggcc gccgaccgcg gcgaactggc accgcgcgcc gactatcccg
    11761 actgggccgg cgcgattgcc gggccgcgca ccgcggaacc cgggcccgct ggcgcacggg
    11821 tcatcctggt tggcagttct gacgtcgcgg tcagcgcgtt cgctcatgcc gaacccggcg
    11881 acgtgatcga gttcctgccg ggaacttatc gattcagcgg ccgcgcgctt gatctctcac
    11941 gccccggccg agccgacgcg ccgatcaccg ttcgcgcccc gcgtctcgga caggcggtgc
    12001 tggaattcgc gatgctcgag ggattccacg tgcgcgcgcc gtactgggtc ttcgagaacc
    12061 tcgtcgtgcg cggcgcatgc gcaacccacg acgactgtga gcacgcgttt cacgtcgtcg
    12121 gcgcagccca cgacatcgtg atccgcaaca acctgctgcg cgacttcaac gcccacatca
    12181 agatcaacgg cgacggcgag cgcttccccg acgcgggaca gatcgtgtcc aacacgctca
    12241 tcaacggccg gccgcgccaa acggacgccc cggtgacacc gatcgacctc gtcgccgcca
    12301 gcggctggac tgtcgaagcc aacctgatcg cggatttcgt gaaggccggc ggggaccaga
    12361 ccagctacgg cggctttgcc aaaggcggcg gtgccggcaa tcgcttcgtt cgtaatgtcg
    12421 tgctctgcga acaccgcctg cgcggagcgc ccggccggcg catcgggctg tcgtttggag
    12481 ggggcggcag cggtaaggcg agctgccgcg accggcgctg cacggtcgag cacgacggcg
    12541 gcgagatctc cggcaatctc gtcatgtcct gttccgacga aggcatctac ttgaaccgcg
    12601 cagctcgtgc acggctgatg cacaacacgg tcatcgacac cgcgggcgtc gtagtgcgct
    12661 atcccgacag ctcggcgcgc gccgacggca atctgatcga cggcgcactg cgcacccgcg
    12721 a
//
LOCUS       DBNHBY010000265         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_692034, whole genome shotgun sequence.
ACCESSION   DBNHBY010000265 DBNHBY010000000
VERSION     DBNHBY010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_692034"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16HB_06550"
     CDS             complement(<1..442)
                     /locus_tag="AC16HB_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606486.1"
                     /translation="MTKMIKLAPLAIAVAVALSACGKKEEPAKKEAAKAAAPAETVVK
                     IGHVAPLTGAIAHLGKDNENGARLAIDEINGAGGIKVGDTTYKLELAAEDDKADPKEG
                     TIAAQKIVDSGAVAVVGHLNSGTSIPASKIYSDVNMTQISPSATN"
     gene            complement(644..952)
                     /locus_tag="AC16HB_06555"
     CDS             complement(644..952)
                     /locus_tag="AC16HB_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5606487.1"
                     /translation="MKQLAILVAAGMTVASGAALANADLAKAKNCLACHAQDKKVVGP
                     AYKDVAAKYASDKDAVAKLSKKIREGGVGVWGQVPMPANPQVNEAEAQTLAKWVLSLK
                     "
     gene            1087..1332
                     /locus_tag="AC16HB_06560"
     CDS             1087..1332
                     /locus_tag="AC16HB_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HIR5606488.1"
                     /translation="MWLLIFAFLLLALKLVGFPPQIEELSWWWVAAPFALAFVWFEFI
                     ERHLGLDKKQAFDELQRTKAKRIKDALARNKHSRVRR"
     gene            complement(1351..>1621)
                     /locus_tag="AC16HB_06565"
     CDS             complement(1351..>1621)
                     /locus_tag="AC16HB_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606489.1"
                     /translation="GHRGHEIFAHVARGDCGLGGNIALIREGDSITIDAYKLLLQLNL
                     SVQQLAHRRSQWKQPAPCYANGVLARFARLASTASGGAITNEERF"
BASE COUNT          235 a          524 c          523 g          339 t
ORIGIN      
        1 ggttggtcgc cgacggcgag atctgcgtca tgttgacgtc ggagtagatc ttcgacgccg
       61 ggatcgaggt gccggagttc agatgcccga ccaccgcaac cgcgcccgag tcgacgatct
      121 tctgcgcagc gatcgtgcct tctttcgggt cggccttgtc gtcctcggcg gcgagttcga
      181 gcttgtaggt cgtatcgccg accttgatac cgccggcgcc gttgatttcg tcgatcgcca
      241 gcctggcgcc gttctcgttg tccttgccga ggtgcgcgat cgcgccggtc agcggcgcca
      301 catgcccgat cttgaccacc gtttccgccg gtgccgccgc cttggccgct tccttcttgg
      361 ctggctcttc cttcttgccg catgcggaca gggcgaccgc gacggcgatc gcgagcggtg
      421 ccagcttgat catcttcgtc atgaaccttt ctccttgacc ctgcgtcaat cactcaaccc
      481 gctcggagtc cagcggccgg cgagcgggcc cggccggccg cgaaagcgcg gcattgtacg
      541 cgccgcgctt cgcgtcgcaa cacgaccgcc ttgccgggaa tcgtgtgtgc aaaagcatcg
      601 gggcgccgca gcgccccgag agaaacccat gtggccgatt cgattacttg agggaaagaa
      661 cccacttcgc cagcgtttgc gcttcggcct cgttgacctg cgggttggcg ggcatcggta
      721 cctggcccca gacgccgacg ccgccttcgc ggatcttctt ggagagcttg gcgaccgcgt
      781 ccttgtcgct ggcgtacttg gcggcgacgt ccttgtatgc gggaccgacc accttcttgt
      841 cctgggcatg gcaggcgagg cagttcttgg ccttggcaag atcggcgttg gcaagggccg
      901 cgccgctggc aacggtcatt ccggcggcga cgaggatggc aagctgtttc atcatggagc
      961 gctccagttt cggtgggggt cacccgttgg tctgcgagtc taacgggtta gtctgatcgg
     1021 cgttgaccgg ggctagtcgc gcgggaatat agttgacgtg gtttttcaca ttgcggaccg
     1081 cgctcgatgt ggctcctgat cttcgccttc ctgctgctcg ccctgaagct tgtcggcttc
     1141 ccgccccaga tagaggagct ctcgtggtgg tgggtcgccg cgccgtttgc gctggccttc
     1201 gtctggttcg aattcatcga acgccatctc gggctggaca agaagcaggc gttcgatgaa
     1261 ctgcagcgca ccaaggcaaa gcgcatcaag gatgcgctcg cgcgcaacaa gcactcgcgc
     1321 gtgcgccgct gatcgcggcc gggcgcgctg ctagaagcgc tcttcgttgg tgatcgcgcc
     1381 cccgctcgcg gtggacgcga gcctggcgaa cctggccagc accccattcg catagcacgg
     1441 cgccggctgc ttccattggc tgcggcgatg cgcaagttgc tggaccgaca ggttcagttg
     1501 cagcagcagc ttgtaagcgt cgatcgtgat cgagtcgccc tcgcggatca gcgcgatatt
     1561 cccgccgagt ccgcaatcac ctcgcgcgac atgggcgaat atttcgtgcc cccggtgccc
     1621 a
//
LOCUS       DBNHBY010000266         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1234437, whole genome shotgun sequence.
ACCESSION   DBNHBY010000266 DBNHBY010000000
VERSION     DBNHBY010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1234437"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="AC16HB_06570"
     CDS             complement(<1..739)
                     /locus_tag="AC16HB_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HIR5606490.1"
                     /translation="MKINWRTVAPLGVWLVLFLIPTPSGLNVNQWHYFAVFAAVIAGL
                     VLESMPVGAVGLIGLTFAAVMGYVEPDPNKSLRWALSGFSESTVWLIVGAFVFSIGYR
                     KSGLGKRIALLLVRALGRRTLGLGYAVTLADLILAPATPSNTARSGGTIYPIISNIPR
                     IYGSEPGPTAGKIGTYVMWTAFAATAVSSSLFLTALAPNAAALAIAKKSVGVDVTWSQ
                     WFVGFAPLGVLLILLVPLLSYIVCRPQI"
     gene            904..>1570
                     /locus_tag="AC16HB_06575"
     CDS             904..>1570
                     /locus_tag="AC16HB_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5606491.1"
                     /translation="MDLNFTPEEEAFRAEVREFARTAMPADLRTAVEQGEHIEREQTI
                     RWQKILHAKGWGAPSWPREWGGPGWDVVRQHIFDEEMALASAPRQLPFGLRMVGPVLM
                     KFGTEAQKRRFLPRIPSMEDWWCQGYSEPGAGSDLASLATRAERKGDRYVVNGQKTWT
                     TLAQHADWIFNLVRTDTTVKKQEGISFLLIDMKSKGVTVRPIELLDGSVEVNEVWFDN
                     VEVP"
BASE COUNT          299 a          507 c          527 g          237 t
ORIGIN      
        1 tgatctgcgg ccggcagacg atgtacgaca gcagcggtac cagcaggatc agcagcacgc
       61 ccaggggcgc gaagccgacg aaccattgcg accaggtcac gtccacgccg accgacttct
      121 tggcaatcgc gagtgcggcg gcgttcggtg ccagcgcggt gaggaacagt gaactggaca
      181 cggcggtggc cgcgaatgcc gtccacatca cgtaggtgcc gatcttgccc gcggtgggtc
      241 ccggctcgga accgtagatg cgcgggatgt tgctgatgat cggatagatc gttccgccgc
      301 tgcgtgcggt gttcgacggc gtggcgggcg cgagaatcag gtccgcgagc gtcacggcgt
      361 aacccagccc cagggtgcgc cgtccgagtg cgcgcaccag caacagcgca atgcgctttc
      421 ccagcccgct cttgcgatac ccgatcgaaa agacgaaagc gccgacgatc agccacaccg
      481 tggattccga aaacccgctg agcgcccagc ggagcgactt attcggatcc ggctcgacat
      541 agcccatcac cgcggcgaaa gtgagcccga tcaaaccgac cgcgccgacc ggcatcgatt
      601 cgagcacgag tcccgcgatg accgccgcga acacggcgaa gtagtgccac tgattgacgt
      661 tgagccccga cggagtcggg atcaggaaca gcaccaacca gacgccaagc ggtgccacgg
      721 ttctccagtt gatcttcatg gtctcccctt cgaagcgcgc atgcggttgg caagctcgct
      781 gctgcctccc gcgcggctcg catcttcgcc tgttcgcaac catccgaagt tgccgcgcgg
      841 ccacaatcgc aatggcgcgc cgcggccgcg tagactcgca gcaactgcga tggcggagtc
      901 attgtggacc tgaacttcac tcccgaggaa gaggcattcc gtgccgaggt gcgcgagttc
      961 gcgcgcactg ccatgccggc cgacctgcgc accgcggtgg agcagggcga gcacatcgaa
     1021 cgtgagcaga cgatccgctg gcagaagatc ctgcacgcca aaggctgggg cgcgccgtcc
     1081 tggccgcgcg agtggggcgg accggggtgg gacgtcgtgc gccagcacat cttcgacgag
     1141 gagatggcgc tcgcctccgc gccgcggcag ttgccgttcg ggttgcgcat ggtcgggccg
     1201 gtgctgatga agttcggcac cgaggcgcag aagcgccgct tcctgccgcg cattccgagc
     1261 atggaggact ggtggtgcca gggctactcg gagcccggcg ccggcagcga ccttgcatcg
     1321 ctcgcgacgc gcgccgagcg caagggcgac cgctatgtcg tcaatggcca gaagacctgg
     1381 accacgctgg cgcagcacgc cgactggatc ttcaatctcg tgcgcaccga cacgacggtg
     1441 aagaagcagg aaggcatcag cttcctgctg atcgacatga agagcaaggg cgtcaccgtg
     1501 cggccgatcg aactgctcga cggctcggtc gaggtcaatg aagtgtggtt cgacaacgtc
     1561 gaggtgccga
//
LOCUS       DBNHBY010000267         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1707899, whole genome shotgun sequence.
ACCESSION   DBNHBY010000267 DBNHBY010000000
VERSION     DBNHBY010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1707899"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="AC16HB_06580"
     CDS             complement(<1..565)
                     /locus_tag="AC16HB_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606492.1"
                     /translation="MIPSLPRSPLTRYGLAAFVTAFFAFLFLPLAVVAVFAFNDANYP
                     APPWQGFTLDWFIGGAARVGLFADQPLLASVGTSALVATCVTVLSVLVGSANAFLLER
                     AQFRGKGLLSLAMLAPLVIPGVILGISILAFASRLAQLADDLWGLELEFLRPGLPLVV
                     LGQFSFIVSIATLTIAARLKRFDLTLEE"
     gene            complement(562..1470)
                     /locus_tag="AC16HB_06585"
     CDS             complement(562..1470)
                     /locus_tag="AC16HB_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606493.1"
                     /translation="MASARAIAPAAPDSGAISASRLMLMLLLAPAALWLLGLIVLPHV
                     ELAALSLRARVAPREYEFSLKQYRTFFEEPLYWNTFVRTATMSIVATACTLALAFPTA
                     WYIAKVARARLQSILLVLCLIPFWVSETVRTLGWMILLRESGVLPRLLVDLGLAEAPP
                     ELLYHDATILVGLVYTSMLFMVIPLVNALQTLDDALVEAAYDLGGNGFDILRRIVIPH
                     AAPGIAAGSIVVFMLTLGNYLTPTLLGGKNSLWFTEQIYTQFITRFNWEQGAAFGFLL
                     LVLSTLIVWSALKLTGQRFGDVMQRS"
     gene            complement(1474..2574)
                     /locus_tag="AC16HB_06590"
     CDS             complement(1474..2574)
                     /locus_tag="AC16HB_06590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606494.1"
                     /translation="MTADLEIDRVSKRFGDYLAVAELSFTVERGSFFSILGPSGCGKT
                     TLLRMIAGFSAPDAGEIRIGGRSMAGVPPNRRPVNMVFQHLALFPMMTVGDNVGYGLT
                     QRGLPRAEITVRVQKMLARVGLAGAQDKRIDQLSGGQQQRVAIARCLVLEPTLLLLDE
                     PLGALDLKLREHMKIELKQLQADFGTTFVYITHDQSEALVLSDRVAVMHAGRFDQIGS
                     PQELYYHPATAFVAGFVGANNRIAGRVERVDDGVALLRTERGLNVRARPAERLAAGDA
                     AEAFVRPEMALLARAAQAAELDSVGQRFEGRVASLLFDGANSAVLVRERASGAEFRIA
                     LPQSGRFADLAVDESIAFGFAPERAVAFKAAG"
     gene            complement(2633..>3214)
                     /locus_tag="AC16HB_06595"
     CDS             complement(2633..>3214)
                     /locus_tag="AC16HB_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5606495.1"
                     /translation="VRLKRPTLIALAFASGKDPFALYKDTKAYTALMEEMGRKLAACK
                     ANLKFFWDNKDQLLNGIRSGEIVGAMMWDTGGWKLNAEKPEIDFIAPKSGALGWVDTF
                     ALPAKGRNDAAAYAWINFNLRPEIAAKVAASAGNFTASSGADQKMTDAKLKAQFAKSF
                     PEAALKNIKWYPAVPAGLEEIEGRILDRVKAGN"
BASE COUNT          568 a         1112 c         1068 g          466 t
ORIGIN      
        1 cttcctccag cgtcaggtcg aatcgcttca ggcgcgccgc gatcgtcagc gtggcgatcg
       61 acacgatgaa cgagaactga cccagcacca cgagcggcag cccggggcgc aggaactcca
      121 gttcgagccc ccacaggtca tccgcgagct gcgccagccg actggcgaac gcgaggatcg
      181 agatgccgag gatcacgcca gggatcacca gcggtgcgag cattgccagc gacagcagtc
      241 ccttgccgcg gaactgcgcg cgctcgagca ggaacgcatt ggcgctgccg accagcaccg
      301 acaaaaccgt gacgcaggtg gcgaccagcg cactggtgcc gacgctcgcg agcagcggct
      361 ggtcggcgaa cagcccgacg cgggccgcgc caccgatgaa ccagtcgagc gtgaagccct
      421 gccaaggcgg cgccgggtag ttggcgtcgt tgaacgcgaa caccgcgacc accgcgagcg
      481 gaagaaacag aaatgcgaag aacgcggtca cgaacgccgc gagaccgtag cgcgtcagcg
      541 gggaacgggg cagggaaggg atcacgagcg ctgcatcacg tcgccgaagc gctgaccggt
      601 gagcttcagc gccgaccaca cgatcagcgt cgacagcacc agcagcagga acccgaaggc
      661 cgcgccttgt tcccagttga agcgcgtgat gaactgggtg tagatctgct cggtgaacca
      721 cagcgagttc ttgccgccga gcagcgtcgg cgtcaggtag ttgccgagcg tgagcatgaa
      781 cacgacgatc gacccggcgg cgatgccggg cgccgcgtgc gggatcacga tccgccgcag
      841 aatgtcgaag ccgttcccgc cgaggtcgta cgcggcctcg accagcgcgt cgtcgagcgt
      901 ctgcagcgcg ttcacgagcg ggatcaccat gaacagcatc gacgtgtaga cgaggccgac
      961 caggatcgtc gcgtcgtggt agagcagttc cggcggcgcc tcggcaagcc cgagatcgac
     1021 cagcaggcgc ggcagcacgc cggattcgcg cagcaggatc atccagccca gcgtgcgcac
     1081 ggtttcgctg acccagaagg ggatcaggca cagcaccagc aggatcgact gcagccgcgc
     1141 acgcgcgacc ttggcgatgt accacgccgt cggaaacgcc agcgccagcg tgcacgcggt
     1201 ggccacgatc gacatcgtcg cggtgcgcac gaaagtgttc cagtacagcg gttcttcgaa
     1261 gaaggtccgg tactgcttca ggctgaattc gtactcgcgc ggcgccacgc gcgcgcgcag
     1321 cgacagcgcc gcaagctcga cgtgcggcag cacgatcagc cccagcagcc acagggccgc
     1381 cggcgccagc agcagcatca gcatcaggcg cgacgcgctg atcgcgcctg agtcgggcgc
     1441 ggcgggcgcg atggcgcgcg cgctcgccat cgctcaaccc gccgccttga acgcgaccgc
     1501 acgctcgggc gcgaagccga acgcgatcga ttcgtcgacg gcgagatcgg cgaagcgtcc
     1561 gctctgcggc agcgcgatgc ggaactccgc gccgctcgcg cgctcgcgca ccagcacggc
     1621 agagttggcg ccgtcgaaga gcaggctcgc gacgcggcct tcgaagcgct gtccgaccga
     1681 gtcgagctcg gccgcctgcg ccgcgcgcgc cagcagcgcc atctccgggc gcacgaacgc
     1741 ttcggccgca tcgccggcgg cgagcctctc cgccggacgc gcgcgcacgt tcagcccccg
     1801 ctcggtgcgc agcagtgcga caccgtcgtc gacgcgctcg actcggcctg cgatgcgatt
     1861 gttggcgccg acgaagccgg ccacgaacgc ggtcgccggg tggtagtaca gctcctgcgg
     1921 cgagccgatc tgatcgaagc ggcccgcatg catcaccgcg acacgatccg acagcaccag
     1981 cgcctcggac tggtcgtgcg tgatgtagac gaacgtggtg ccgaagtcgg cctgcaactg
     2041 cttcagctcg atcttcatgt gctcgcgcag cttcagatcc agcgcgccga gcggctcgtc
     2101 cagaagcagc agcgtcggct ccagcaccag gcagcgcgcg atcgccacgc gctgctgctg
     2161 cccgccggag agctggtcga tgcgcttgtc ctgcgcgccc gcaagcccca ctcgcgccag
     2221 catcttctgc acccgcaccg tgatctcggc gcgcggcagt ccgcgctgcg tgagtccata
     2281 gccgacgttg tcgccgaccg tcatcatcgg aaacagcgcc aggtgctgga ataccatgtt
     2341 gaccggccgc ctgttgggcg gcacgcccgc catcgagcgg ccgccgatgc ggatctcgcc
     2401 agcgtccggt gcgctgaagc ccgcgatcat tcgcagcagc gtcgtcttgc cgcagcccga
     2461 cggcccgagg atcgagaaga agctgccgcg ctcgaccgtg aatgacagct cggcgaccgc
     2521 gaggtagtcg ccgaagcgct tgctgacacg gtcgatctcg aggtcggcgg tcatcacgca
     2581 acaaaaacgg cgcatgggtg gatgcgccgt tgcggtgaag aaaggactgg cttcagttcc
     2641 ccgccttcac gcggtcgagg atgcggccct cgatctcctc cagcccggcg ggcacggcgg
     2701 gataccactt gatgttcttc agcgccgctt cggggaagct cttggcgaac tgcgccttga
     2761 gcttcgcatc ggtcatcttc tggtcggcgc cactggatgc ggtgaagttg ccggccgatg
     2821 ccgccacctt ggcggcgatc tccggccgca ggttgaaatt gatccacgcg tacgcggccg
     2881 cgtcgttgcg acccttggcc ggcagcgcga aggtgtcgac ccagccgagc gcgccggact
     2941 tcggagcgat gaagtcgatc tccggcttct cggcgttgag cttccagccg ccggtgtccc
     3001 acatcattgc gcccacgatc tcgccgctgc ggatgccgtt cagcaactgg tccttgttgt
     3061 cccagaagaa cttgaggttg gccttgcacg cggcgagctt cctgcccatc tcctccatca
     3121 gcgccgtgta cgccttggtg tccttgtaca gtgcgaacgg atccttgcca ctggcgaacg
     3181 ccagcgcgat cagcgtcggc cgcttcagcc gcac
//
LOCUS       DBNHBY010000268         1566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1030747, whole genome shotgun sequence.
ACCESSION   DBNHBY010000268 DBNHBY010000000
VERSION     DBNHBY010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1030747"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC16HB_06600"
     CDS             <1..726
                     /locus_tag="AC16HB_06600"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5606496.1"
                     /translation="FLEELTWTELRGELATGKTTILIPIGGTEQNGPAMVLGKHNVRV
                     KLLAERIARALGNALVAPVLAYVAEGNIDPPTAHMRFPGTITLPEATFEKVIESAARS
                     LQHHGFRDIVLLGDHGGYQKNLQAVAARLNRAWAASGARVHAISDYYRATEDAYPQLL
                     KRRGFRDEEIGTHAGLADTSLALALDPRLVRGERLQAEPHAGRAEGVYGDPRRASAEL
                     GKFGVDLIVARSVDAIRKAVARH"
     gene            755..>1566
                     /locus_tag="AC16HB_06605"
     CDS             755..>1566
                     /locus_tag="AC16HB_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5606497.1"
                     /translation="MKRHAMKMHRRFAPLALAAVTVGVGAQMPPQPDPNNLYSETVAG
                     KMSPAVKDHLPRIYVPNRQSNDVTVIDPATLKVVDRIKVGINPQHIVPSWDLQTLWVT
                     NNAEGRTDGTLTPIDPKTGKRRGPDVVVDDPYNMYATPDGASAIVVAEARKRLDFRDP
                     HTMKLQYSIDVPQCPGINHADFSIDGKYAIFTCEYSGGLAKIDLANRKVLGYLSLGKG
                     RMPQDVRASPDGKIFYVADMKSDGVHLVDGESFTKIGFIATGVGAHGLYPSR"
BASE COUNT          289 a          533 c          513 g          231 t
ORIGIN      
        1 ttcctcgagg agctgacctg gaccgagctg cgcggcgagc ttgcgacggg caagaccacg
       61 atcctcattc ccatcggcgg taccgagcag aacgggccgg cgatggtgct cggcaagcac
      121 aacgtgcggg tgaagctgct ggcggagcgg atcgcgcgcg cgctcggcaa tgcgctggtg
      181 gcgccggtgc tcgcgtacgt ggctgaaggc aacatcgatc cgccgaccgc gcacatgcga
      241 ttccccggca cgatcacgct gcccgaagcc accttcgaga aggtgatcga gtcggcagcg
      301 cgcagcctgc agcaccacgg cttccgcgac atcgtgctgc tcggcgacca cggcggctac
      361 cagaagaacc tgcaggcggt cgcggcgcgg ctgaaccgcg cgtgggccgc gagcggcgcg
      421 cgagtgcacg cgatcagcga ttactaccgc gccaccgagg acgcgtatcc gcagttgctg
      481 aagcggcgcg gctttcgcga cgaggagatc ggaacccacg cggggctcgc cgacacttcg
      541 ctcgcgctgg cgctcgatcc gcggctggtg cgcggcgagc ggctgcaggc cgagccgcac
      601 gccggtcggg ccgagggcgt ctacggcgac ccgcgacgcg ccagcgcaga gctcggcaag
      661 ttcggcgtcg acctgatcgt cgcgcgcagc gtcgacgcga ttcgaaaagc ggtcgcacgc
      721 cattaggaat gcaggtacac cggatttcga cctcatgaag cgacatgcaa tgaagatgca
      781 ccgccgattc gccccccttg cgcttgccgc cgtcaccgtc ggcgtcggcg cacagatgcc
      841 gccgcagccc gatccgaaca atctgtatag cgagaccgtt gccggcaaga tgagccccgc
      901 ggtcaaggat catctgccgc gcatctacgt gcccaaccgt caatcgaacg acgtgaccgt
      961 gatcgacccg gcgacgttga aggtggtcga ccgcatcaag gtcggcatca atccgcagca
     1021 catcgtgccg tcgtgggacc tgcagacgct gtgggtcacc aacaacgccg agggtcgcac
     1081 cgacggcacg ctgacgccga tcgacccgaa gaccggcaag cggcgcgggc ccgacgtcgt
     1141 cgtcgacgat ccgtacaaca tgtacgccac gcccgatggc gcctcggcca tcgtcgtcgc
     1201 ggaagcgcgc aagcggcttg acttccgcga tccgcacacg atgaagctgc agtactcgat
     1261 cgacgtgccg caatgcccgg gcatcaacca cgcggatttc tcgatcgacg gcaagtacgc
     1321 gatcttcacc tgcgagtatt ccggcggcct ggccaagatc gatctcgcca atcgcaaggt
     1381 gctcgggtac ctgtcgctcg gcaagggacg gatgccgcag gacgtgcgcg cgtcgcccga
     1441 cggcaagatc ttctatgtcg ccgacatgaa atccgacggc gttcatctgg tcgacggcga
     1501 atcgttcacc aagatcggct tcatcgccac cggcgtcggc gcgcatggct tgtacccgag
     1561 ccgcga
//
LOCUS       DBNHBY010000269         2283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_490030, whole genome shotgun sequence.
ACCESSION   DBNHBY010000269 DBNHBY010000000
VERSION     DBNHBY010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_490030"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1115
                     /locus_tag="AC16HB_06610"
     CDS             132..1115
                     /locus_tag="AC16HB_06610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5606498.1"
                     /translation="MLNTLLRLTVKPRWKPGVEIAEIRRRASASDRRFGGRGPDIPSE
                     DVMIAGCAARWFGAPELATQRGTLLYLHGGAWVLHLPALYQRFAAELSVATGLRVLLV
                     DYRLAPENPFPAATDDCLAVYRALVENGLSPVAVAGDSAGGSLTLVTLMRARDAGLPM
                     PACAVLLSPSTDLTLSGASAHYNAEADPMFSRVAGDVLPAFYCPGEARQHPWLSPLFG
                     QWHGLPALYFIAASTEMLLDDSIRAQDRAVQAGVEARIDVWPEVPHVFPLFSFLPEAR
                     QAVAQIVEFLNAHVDRRAAAVEHSRPLLIAEPHEAAVRQVETATAQEASSL"
     gene            complement(1116..2066)
                     /locus_tag="AC16HB_06615"
     CDS             complement(1116..2066)
                     /locus_tag="AC16HB_06615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5606499.1"
                     /translation="MSPEPLPADPTEASPRRSVAERVVAGVLRKVLAATLLPTFTPER
                     PIESQRRRLEKISALTLAPRAVYAAAEYGGVRGESVTARGQGEPDRAILYLHGGAYCV
                     GSPRTHRPITGTLARLTGARVFAVDYRLAPEHPFPAALDDALAAYRGLLAQGYPAQRV
                     AFAGDSAGGGLALASAIRARELSVPLPAALVAFSPWVDLGAEHDGVPPGEVMISPPWV
                     AECARLYLAGREAREPLASPVYGQLQGLPPTLLQVGTDEVLLADSRRMRDALRAAGVS
                     VSYQEYARRWHVFQVNAGMLADADRALASVADFLRERWAV"
BASE COUNT          350 a          822 c          794 g          317 t
ORIGIN      
        1 gctgcagcgc gccatggcgg cgtccgccga cgcccggcct gcgtgaacgc cctgatgcgg
       61 ggccacaccg caccgccgac aatgccgtca cactgaaaga gcgatcgccc atgcccagtc
      121 tgcgcgcacg tatgctgaac accctgctgc gcctcacggt caagccgcgc tggaagcccg
      181 gcgtcgagat cgcggagata cgccggcggg cgagcgcctc ggatcgacgg ttcggcggcc
      241 gcggcccgga catacccagc gaagacgtca tgatcgcggg ttgtgccgcg cgctggttcg
      301 gcgcgccgga actggcgaca cagcgcggaa cgctgctcta cctgcacggc ggcgcgtggg
      361 tgctgcacct gcccgcgctg taccagcgct tcgccgccga actcagcgtg gccacggggc
      421 tgcgggtgct gctggtcgat taccggctgg cgccggagaa ccccttcccg gcggctacgg
      481 acgactgcct cgcggtctat cgcgcgctcg tggaaaacgg gctcagtccc gtcgcggtcg
      541 ctggcgattc ggctggaggc agcctgacgc tcgtcaccct gatgcgcgcg cgcgatgccg
      601 gcctgccgat gcccgcgtgc gcggtactgc tctcaccttc gaccgacctc acgctcagcg
      661 gcgcttcggc gcactacaac gcagaggccg atccgatgtt cagtcgtgtg gccggcgacg
      721 tgctgcccgc gttctactgc ccgggcgagg cacgacagca tccgtggctg tcgccgctgt
      781 tcggccaatg gcacggcctg ccggcgctct atttcatagc ggcgtcgacc gagatgctgc
      841 tcgacgactc gatccgcgcg caggaccgcg cggtgcaggc cggcgtcgaa gcgcgcatcg
      901 acgtatggcc cgaggtccct cacgtgttcc cgctgttctc gttcctgccc gaagcccggc
      961 aagccgtcgc gcagatcgtc gagttcctca atgcgcacgt cgatcggcgt gcggccgcag
     1021 tcgagcactc gaggccgctg ctgatcgcgg agccacacga ggccgccgtg cggcaggtcg
     1081 aaaccgccac ggcgcaagag gcgtcctcgc tgtaactaga ccgcccagcg ctcgcgcagg
     1141 aaatcggcaa ccgacgcgag cgcgcgatcc gcgtccgcca gcatgcccgc gttgacctgg
     1201 aacacgtgcc agcgtcgcgc gtattcctgg tagctgacgc tgacgcccgc agcgcgcaga
     1261 gcgtcgcgca tgcgtcgcga atcggccagc agtacctcat cggtgccgac ctgcagcagc
     1321 gtcggcggca atccttgcag ttggccgtag acaggggacg cgagcggctc ccgcgcctcg
     1381 cgaccggcga ggtacaggcg tgcgcactcg gccacccagg gcggcgagat catgacctcg
     1441 cctggcggca cgccgtcatg ctcggcgcca aggtcgaccc acggggaaaa cgccacgagc
     1501 gccgcaggca acggcacaga cagttcgcgc gcgcgaatcg cgctggcgag agcgagcccg
     1561 ccgcctgcgg agtcgccggc gaacgccact cgttgcgccg ggtagccctg cgcaagcagt
     1621 ccgcggtacg cggccagcgc atcgtcgagt gcggctggaa aaggatgctc gggtgccagc
     1681 cggtagtcga ccgcaaagac ccgcgcacct gtcaggcgcg cgagtgtgcc cgtgatcgga
     1741 cggtgcgtgc gcggcgaacc cacgcagtaa gcgccgccgt gcagatagag gatggctcga
     1801 tcgggctcgc cctgcccgcg cgccgtaacc gattcgcctc gcacgccacc gtactctgcc
     1861 gccgcataga cggcgcgagg ggccagggtc agcgcactga tcttctcgag gcggcgccgc
     1921 tgcgactcga tcggtcgctc cggcgtgaag gtgggcagca gcgtggccgc gagcaccttg
     1981 cgcagtacgc ctgcgacgac gcgctccgcg acgctccgcc gcggcgaagc ctctgtcggg
     2041 tcagcgggca acggctcggg gctcatgccg cgcaacgata ccgcaacagc ccgccacaac
     2101 gggcgtgaag cggccgctgc gccggggcca cgattagaga tcgatatcag tccgagaggc
     2161 ggcgcgaatg cgcgcgccgg aggtcagcgc ggctcgccga tgacctcgat gcgtgccttg
     2221 gtgacgccgc ggcgcaccag gccgatctgc tccgccgccg cgtgcgagag gtccgcgatg
     2281 cgc
//
LOCUS       DBNHBY010000270         7393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1234653, whole genome shotgun sequence.
ACCESSION   DBNHBY010000270 DBNHBY010000000
VERSION     DBNHBY010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1234653"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="AC16HB_06620"
     CDS             <1..649
                     /locus_tag="AC16HB_06620"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5606500.1"
                     /translation="FVHVFQEEAAQLQGRGTAAKWLAQGTIYPDVIESAGAKTKKATT
                     IKSHHNVGGLPESLHLKLLEPLRELFKDEVRALGVALGLPHAMVYRHPFPGPGLGVRI
                     LGEVKREFADLLRRADAIFIDELRASGWYDKTSQAFAVFLPVKSVGVMGDGRTYDHVV
                     ALRAVQTQDFMTAHWAHLPHDLLARVSNRIINEVRGINRVVYDISGKPPATIEWE"
     gene            812..1204
                     /locus_tag="AC16HB_06625"
     CDS             812..1204
                     /locus_tag="AC16HB_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5606501.1"
                     /translation="MEARHMRSTRNVIDSHLKCFIERDLGGTLADYAPGAVLFTPDGP
                     LRGVDAIRPFFQALLSEFGKPGATFSLRHLSVEGEHAYILWTGETADNVYELGTDTFV
                     VRDGKIAIQSFAGKLTPRRGSIASVRPG"
     gene            1226..2161
                     /locus_tag="AC16HB_06630"
     CDS             1226..2161
                     /locus_tag="AC16HB_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5606502.1"
                     /translation="MAASPSILASVGGTPLLELRRVVPAGCARVLVKLESHNPTGSMK
                     DRMALAVVEGAARAGRLAPGGTVVEYTGGSTGTSLAFVCAALGYRVTLVSSDAFSREK
                     RDHMRALGATVIELPSEGGRTTKELIQRMIAKAAELTDAPNVFFADQFNNPDAAAGYA
                     PMAEEMWEQSGQALDAFVQSVGTAQCITGAARVLRRQNPGIQIAAVEASESPVLSGGA
                     PGAHKIEGVGPGFVPPLWQADLADEVHQVSTADAKDMARRLAKEEALFAGTSSGANVV
                     AALRIARRLGPARTVATLVCDTGLKYLSTDLYSAG"
     gene            2337..2513
                     /locus_tag="AC16HB_06635"
                     /pseudo
     CDS             2337..2513
                     /locus_tag="AC16HB_06635"
                     /inference="COORDINATES: protein motif:HMM:NF021386.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
     gene            2673..3197
                     /gene="tadA"
                     /locus_tag="AC16HB_06640"
     CDS             2673..3197
                     /gene="tadA"
                     /locus_tag="AC16HB_06640"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR5606503.1"
                     /translation="MEGAPPPLDQPDDETYMRLALDQSNNAWALGEVPVGAVLVKDGQ
                     VLATGFNQPIGNSDPTAHAEIQTIRAAAELLGNYRLSHCTLYVTLEPCAMCAGAIQHA
                     RIARLVYGASDPKTGACGSVVDLFAEPKLNHNAQVRRGVLADACARVLSDFFAQRREL
                     QRAGSLPADDDEAN"
     gene            3202..4101
                     /locus_tag="AC16HB_06645"
     CDS             3202..4101
                     /locus_tag="AC16HB_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HIR5606504.1"
                     /translation="MRAHAIGLVAPSGRLPHRRIADRAARFFARRGWRVQAGESVFAR
                     ETRFAGSDELRAADLQRFATDRSLDVVLAARGGYGLSRILDRLDYAAIARAKRIVVGY
                     SDFTAFNLALLAKTGAISFQGPAATDFAAEKPDGFAVEHFFAALENRRHALKFDADGP
                     NASVKGTLWGGNLALVCALLATPYFPKVRGGILFLEDVSEAAYAIERMLLQLAHADVL
                     ARQRAIVLGAFDPVRPMPNDYGYDLRAAIARVRAAVPVPIVTGLPFGHVARKATLPVG
                     AAAQLIVRDGEAELAFSGHPTLK"
     gene            4148..6064
                     /locus_tag="AC16HB_06650"
     CDS             4148..6064
                     /locus_tag="AC16HB_06650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR5606505.1"
                     /translation="MAGPITRFGIEIESREQRQEKILAGVIASVYRQVPQSVLGSIVG
                     GVALVAVFWKTHNPNVLLAWFIAMLVESLVRLRVARAFRAATATADRIRMWSARWVTL
                     AAVAGVLWGAAGVLFFAPREPLQQLVLVAVILGVAFGSLTLYATHRPALFAFLPAALL
                     PLIARMVAQGEPAYYTAAIVVLAVFGFTMFFGRNFGETMFEAVKNNYENEVLVAQLMN
                     EKRVADEARRAAEDATRSKTKFFAAASHDLRQPLQAIGIYCSLLKKRAQGPLEPLVKN
                     LSSAVDSLSKLVEELLEISRLDAGAIQPRVQPVLIDEMFTALEQEFMPQAAGKGLALR
                     VRQAGIAVESDPVLLSRVLRNLLGNALRYATRGGVLLAARPRGGLISLEVWDTGPGIR
                     KEELDRIFEEFYRGESSRGEAASGFGLGLSIVKRICSVLGHPLIVTTRPGSGTVFRVE
                     VPLSSSPRPAARSANELTDASLRSLTGHSIVLIEDNEEILNSMSNLLRSWGAEVVAAT
                     GFNEALVADLKACKRVDLVLADQNLRDSISGVEAVFRVREIVGFPVPVVMLTAVQGIE
                     VVGEFQRTLQRHLQANPQLASIIARSRVEEPLVLQKPTNAIVLNNRIAGALGLLVSGL
                     PLVTRQSNEISLAG"
     gene            complement(6076..6744)
                     /locus_tag="AC16HB_06655"
     CDS             complement(6076..6744)
                     /locus_tag="AC16HB_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5606506.1"
                     /translation="MKILLVDDHALITNAMTTLLLDLDPKTEIHTASDAAGAERLVEQ
                     HPDADLMLLDLGLPGTTGTNLLEALVGRAPDLKILVLSGVQDQRTVMRVLQLGAAGFL
                     PKSMATETLINAVRFVMSGGVYIPADLLEDATRGASMLGLPERGRDMLGRPTSGRVQL
                     TERQEQVLQLLARGSPIKIICRELGLSEGTVKTHVTAIYRAFGASNRTEALLAARRNG
                     YDVG"
     gene            complement(6861..>7393)
                     /locus_tag="AC16HB_06660"
     CDS             complement(6861..>7393)
                     /locus_tag="AC16HB_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5606507.1"
                     /translation="CEPIGHVQVMGDYVESWQPHPMRPTALAAAREYARRVTDDLGGV
                     GIFGVELFVKGDKVWFSEVSPRPHDTGLVTLCTQWQSEFELHARALLGLPVDPSLRNA
                     GASHVIYGGVDARAVIFDGVADALAVPGADVRLFGKPESYVRRRMGVALARAESVEQA
                     RERARQAAAQVRPRAA"
BASE COUNT         1157 a         2393 c         2601 g         1242 t
ORIGIN      
        1 attcgtgcac gtgttccagg aggaagcggc gcagctgcag ggtcgaggaa ccgcggcgaa
       61 gtggctggcg caggggacga tctacccgga cgtgatcgaa tcggccggcg cgaagaccaa
      121 gaaggccacc acgatcaaga gccaccacaa cgtcgggggg ctgcccgagt cgctgcacct
      181 gaagctgctc gaaccgctgc gcgaactgtt caaggacgag gtgcgggcgc tcggcgtcgc
      241 actcggtttg ccgcacgcga tggtgtaccg gcacccgttc cccggtcccg ggctgggcgt
      301 gcgcatcctc ggcgaggtga agcgcgagtt cgccgacctg ctgcggcggg cggacgcgat
      361 tttcatcgac gagttgcgtg ccagcggctg gtacgacaag accagccagg cgtttgcggt
      421 gtttctgccc gtgaagagcg tgggcgtgat gggcgacggc cgcacctacg accacgtggt
      481 cgcgctgcgc gcggtgcaga cgcaggactt catgaccgcg cactgggcgc acctgccgca
      541 cgacctgctg gcgcgcgtgt cgaaccgcat catcaacgag gtgcgcggca tcaaccgcgt
      601 cgtctacgac atctcgggca agccgccggc gacgatcgag tgggagtagg ttcggtcggc
      661 tgattcgcgt cgaagaagcg tcatccggcg caagttccgt acgcgcgaac aaggttccag
      721 gacgttcggt gaagcgcgaa tggcttccgg cgcgaacaat cgggtcgtcg catgaggcga
      781 ccggtcgcgc agcgaccaat cttttcctgc aatggaggct cggcatatgc gttcgacgag
      841 gaatgtcatc gacagtcatt tgaagtgctt tattgagcgt gatctcggag gcacgctcgc
      901 cgactatgcg ccgggtgcgg tgctgttcac gcccgacggg ccgctgagag gcgtcgacgc
      961 gattcggccg tttttccagg cgctgctttc ggagtttggc aagcccgggg caacgttcag
     1021 cctgcggcac ctgtccgtcg agggcgagca tgcctacatc ctgtggaccg gagagactgc
     1081 agacaacgtg tacgaactgg gcacggacac gttcgtcgtg cgtgacggca agatcgcgat
     1141 tcagtcgttc gcgggcaagc tcacacccag gcgcggctcg atcgcgtcgg ttcggccggg
     1201 atagggctgc ggcggccgtc ggctcatggc tgcgtcgccg agcatccttg cttccgtcgg
     1261 aggaacgccg ctgctggaac ttcggcgcgt cgttcccgcc ggctgcgccc gtgtgctggt
     1321 gaagctcgaa agccacaatc cgacgggaag catgaaggac cgcatggcgc tcgccgtcgt
     1381 ggaaggtgcc gccagggccg ggcggctggc accaggcgga acggtcgtcg aatacaccgg
     1441 cggcagcacc ggaacgtcgc tggccttcgt gtgcgccgcg ctgggctatc gagtcacgct
     1501 cgtcagttcg gatgcgttca gccgcgagaa gcgcgatcac atgcgggcgc tcggagcgac
     1561 ggtgatcgag ctgccgagcg aaggcgggcg cacgacgaag gaactgatcc agcgaatgat
     1621 tgcgaaagcg gctgaactga ccgacgcgcc gaacgtcttc ttcgccgacc agttcaacaa
     1681 tccggacgcc gccgccggtt acgctcccat ggcggaggag atgtgggagc aatcgggaca
     1741 agcgctcgac gcgtttgtcc agtcggtcgg gaccgcgcag tgcatcactg gggctgcgcg
     1801 cgttctccgg cgccagaatc cgggaattca aattgccgcc gtcgaagcga gcgaatcgcc
     1861 ggttctgagc ggcggcgcgc ccggtgcgca caagatcgag ggcgttggtc ccggcttcgt
     1921 tcctccgcta tggcaggccg atctcgccga tgaggtccat caagtctcca cggccgacgc
     1981 gaaagacatg gcgcgacggc tggcgaaaga ggaagcgctg tttgccggga cctcttccgg
     2041 ggccaatgtc gtcgcggcgc tgcgaattgc ccgccgtctc ggaccggccc ggaccgtggc
     2101 gaccctcgtc tgcgacacgg gactgaagta tctgtcgacc gacctgtact cggccggcta
     2161 gccgcgctca caaagtaaac tgcgcacggt ccgatgaccg agcaggagtg gccggcgtgc
     2221 agctcgccgc gagtatttcc gaggacgggt ggctggagcg gctggcgcag ctcgcggtgc
     2281 gcgacggcgt tcatctggcc acgctgcgcc agcgcgatgc gcgcgatttc gaactggtgc
     2341 tggcgtcggc cacattgttc ctcccgcccg gccagttgct gaccgagcgc gaagcgaacg
     2401 acgtcctgag ggagtttctt tcgtcggcag gcgcgatgct cgcgaccgat cacgtcgaac
     2461 tgcggcgctg gctcgctgac acgggcttcg tgcatcggtc cgaccgcggc accgactatc
     2521 gccgcggcac gttgcccgac tggcttgtgg ccgcagcgga cagcctcact gcggcacggc
     2581 tgcgcgatga agtgctcgcg gcgcgcaacg cgaagggcag ggcacgcgcg gcgcgcaagc
     2641 agtcctggct ggagcagggc gcggtcgagg agatcgaagg tgcgccaccg ccgctcgatc
     2701 agcccgacga cgagacctac atgcggcttg cgctcgatca gtccaacaac gcgtgggcgc
     2761 tgggcgaagt gccggtcggt gcggtgctgg tcaaggacgg ccaggtgctc gccacgggat
     2821 tcaaccagcc gatcggcaat tccgatccga ccgcacacgc ggagattcag acgatccgtg
     2881 ccgcggccga gttgctcggc aactaccggt tgagccattg cacgctgtac gtgacgctgg
     2941 agccgtgcgc gatgtgcgcc ggcgcgatcc agcatgcgcg cattgcgcgg ctcgtctatg
     3001 gcgccagcga tccgaagacc ggcgcctgcg gcagcgtggt ggatctcttc gcggagccga
     3061 agctgaacca caatgcgcag gtgcgccgcg gcgtgctggc ggatgcgtgc gcccgggtgc
     3121 tgtccgactt cttcgcgcag cgccgcgaac tgcagcgcgc gggttcattg ccggccgacg
     3181 acgacgaggc gaactgagcc gatgcgtgca cacgcgatcg gcttggtcgc accctccggc
     3241 cgtcttccgc accgacgcat cgccgatcgc gccgcacgtt tcttcgcgcg gcgtggttgg
     3301 cgcgtgcagg caggcgagag cgtgttcgcg cgcgagacgc gcttcgccgg cagcgacgag
     3361 ttgcgcgcgg ccgacctgca gcgattcgcg accgatcgct cgctcgacgt ggtgttggcg
     3421 gcgcgcggcg gctacgggct gtcgcgcatc ctcgatcggc tggattacgc ggcgatcgcg
     3481 cgcgcgaagc gcatcgtcgt tggctacagc gacttcaccg cgttcaatct tgcgctgctg
     3541 gcgaagactg gcgcgatcag cttccagggt ccggcggcga ccgatttcgc tgccgagaag
     3601 cccgacggat ttgcggtcga gcatttcttc gctgcgctcg aaaaccgacg gcatgcgctg
     3661 aagttcgatg cggacggccc caacgcatcg gtgaagggaa cgctgtgggg cggcaacctt
     3721 gcgctggtct gcgcgctgct ggccacgccg tacttcccca aggtccgcgg cggcatcctg
     3781 ttcctcgagg acgtgagtga agccgcctac gcgatcgagc gcatgctgct gcaactggca
     3841 cacgcggacg tgctggcgcg tcagcgcgcg atcgtgctcg gcgcattcga tccggtcagg
     3901 ccaatgccga acgattatgg ctacgatttg cgggcggcca tcgcacgcgt ccgcgccgcg
     3961 gtgccggtgc ccatcgtcac gggcctgccg tttgggcacg tggcgcgcaa ggccacgctg
     4021 ccggtcggcg cggccgctca gctcatcgtg cgcgacggtg aggccgagct tgcgttcagc
     4081 gggcatccga cgctgaagtg agcgtgccgc gcttcgcgca gcggcgccgc tcgcctatca
     4141 tgcggccatg gccggcccga taacccgctt tggcatcgaa atcgagtcgc gcgagcagcg
     4201 ccaggagaag atcctcgccg gcgtcatcgc gtcggtgtat cgccaggtcc cgcagtcggt
     4261 gctgggcagc atcgtcgggg gggtcgcact ggtcgctgtc ttctggaaga cgcacaaccc
     4321 gaacgttctg ctggcctggt tcatcgccat gctggtcgaa tcgctggtgc ggctgcgcgt
     4381 tgcgcgcgcg ttccgggcgg caacggcgac cgcggatcgg atccgcatgt ggtcggcccg
     4441 ttgggtcacg ctggctgccg ttgcgggcgt gctgtggggc gcggccgggg tgctgttctt
     4501 cgcgccgcgg gaaccattgc agcaactggt gctggtcgcg gtgatcctcg gtgtggcgtt
     4561 cggctcgctg acgttgtatg cgacgcatcg gccggcgctg ttcgcgttcc tccccgcggc
     4621 gctgctgccg ctgatcgcgc gcatggtcgc gcagggcgag ccggcctact acaccgctgc
     4681 gatcgtcgtg ttggcggtgt tcggcttcac catgttcttc ggccgcaact tcggcgagac
     4741 gatgttcgag gcggtgaaga acaactacga gaacgaggtg ctggttgcgc agctgatgaa
     4801 cgagaagcgt gtggccgacg aagcacgccg cgccgccgaa gacgccacgc gttcgaagac
     4861 gaaattcttc gctgccgcca gccacgatct gcggcagccg ctgcaggcga tcggtatcta
     4921 ctgctcgctg ctgaagaagc gcgcgcaggg gccgctggag ccactcgtga agaacctgtc
     4981 gagcgccgtc gactcgctgt ccaagctggt cgaggagttg ctcgaaatct cgcgccttga
     5041 cgccggtgcg atccagccgc gggtgcagcc ggtgctgatc gacgaaatgt tcacggcgct
     5101 ggagcaggaa ttcatgccgc aggccgccgg caaggggctg gcactgcgcg tgcggcaggc
     5161 gggcatcgcg gtggaatccg atccggtgct tctctcgcgc gtgctgcgca acctgctcgg
     5221 caatgcgctg cgctatgcga cacgcggtgg cgtgctgctg gcggcgcgtc cgcgcggcgg
     5281 cctgatcagc ctggaggtat gggacaccgg accaggcatc cgcaaggaag agctcgaccg
     5341 catcttcgag gaattctatc ggggcgagag ttcgcgcggc gaggcggcga gcggcttcgg
     5401 gctcgggctg tcgatcgtca agcgtatctg ctcggtgctc ggccatccgc tgatcgtcac
     5461 cacgcggccg ggctcgggaa cggtgttccg cgtcgaggtg ccgctgtcgt cgtcgccgcg
     5521 gccggcggcg cgcagcgcga acgaactcac ggacgcgtcg ctgcgctcgc tgaccgggca
     5581 ttcgatcgtg ctgatcgagg acaatgagga gatcctgaac agcatgagca acctgctgcg
     5641 ctcgtggggt gcggaagtcg tcgctgcgac cggcttcaac gaagcgctgg tggcggatct
     5701 gaaggcgtgc aaacgggtcg acctggtcct tgccgaccag aatctgcgcg actcgatctc
     5761 gggcgtcgag gcggtcttcc gcgtgcgcga gatcgtcggc ttcccggtgc cggtcgtgat
     5821 gctgacggcc gtgcagggga tcgaagtggt gggcgagttc cagcggacgt tgcagcgcca
     5881 cttgcaggcg aacccgcaac tggcgtcgat catcgcgcgc agccgcgtcg aggagccgct
     5941 ggtgctgcag aaaccgacca acgcgatcgt tctgaacaac cgcatcgcgg gcgcgctcgg
     6001 attgctggtt tccggcctgc cgctggtgac gcggcagtcg aacgaaatca gcctggcggg
     6061 ctgagccgcc ggcggtcagc cgacgtcgta gccgttgcgg cgtgcggcga gcagcgcctc
     6121 ggtgcggttc gacgctccga acgcgcgata gatcgcggtc acgtgtgttt tcaccgtgcc
     6181 ttcgctgagt ccgagctcgc ggcagatgat cttgatcgga gagccacgtg ccagcagctg
     6241 cagcacctgc tcctggcgtt cggtgagctg cacgcggccc gaggtcggac ggccgagcat
     6301 gtcgcggccg cgttccggca gacccagcat cgatgcgccg cgcgtcgcgt cctccaacag
     6361 gtcagccgga atgtagacgc cgcccgacat cacgaagcgc accgcgttga tcaacgtttc
     6421 ggtcgccatc gatttcggca agaagcccgc ggcgccaagc tgcagcacgc gcatgacggt
     6481 gcgctggtcc tgcacacccg acagcacgag gatcttcagg tcgggcgcac gcccgaccag
     6541 cgcctcgagc agattcgtcc cggtcgttcc cggcaggccg aggtcgagca gcatcaggtc
     6601 cgcgtccggg tgctgctcga ccagccgctc tgctcccgcg gcatcgctgg ccgtgtgaat
     6661 ctcggtcttg gggtcgagat cgagcagcag cgtcgtcatt gcgttggtga tcaacgcgtg
     6721 gtcatcaacc agcagaatct tcatcgcgcg cctgtcggtc ctgtagtcgt tgaattctgg
     6781 aatgcgggtc ggccgcttcg agcgtgcatt ctaacgacac atgcacgctg gcatgttggc
     6841 accccgagcc gatcgcgcac tcacgccgcg cgtggccgta cctgcgccgc cgcctgccgg
     6901 gcgcgctcgc gcgcctgctc gaccgactcg gcgcgcgcca gtgccacgcc catgcgccgc
     6961 cggacatacg actccggctt gccgaacagg cgcacgtcgg cgcccggcac ggcgagtgca
     7021 tcggccacgc cgtcgaagat caccgcccgg gcgtcgacgc cgccatagat cacgtggctc
     7081 gcacccgcgt tgcgcagcga gggatcgacc ggcaggccga gcagggcgcg cgcgtgcaac
     7141 tcaaactcgc tctgccactg ggtgcacagc gtcacgaggc cggtgtcgtg cgggcgcggg
     7201 ctgacctccg agaaccagac cttgtcgcct ttgacgaaca actcgacgcc aaagatgccg
     7261 accccgccca ggtcgtcggt gacgcgccgg gcgtattcgc gcgccgcggc gagcgctgtc
     7321 gggcgcatcg gatgcggctg ccagctttcc acatagtcgc ccatcacctg cacgtggccg
     7381 atcggctcgc aga
//
LOCUS       DBNHBY010000271         5914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1505177, whole genome shotgun sequence.
ACCESSION   DBNHBY010000271 DBNHBY010000000
VERSION     DBNHBY010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1505177"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="sctC"
                     /locus_tag="AC16HB_06665"
     CDS             <1..1049
                     /gene="sctC"
                     /locus_tag="AC16HB_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HIR5606508.1"
                     /translation="NAVIVRDVAERLPRYDQLIAALDVEPQSLEIEATIIDVNVDRLR
                     ELGVNWRWNNAGREVGYNGGVPTAGVGGIASAVLGSVGQFVARIRALQAEGAARIVSS
                     PQVVTLSDVEAVFDNSSTFYVRVAGREQVDLFNVSAGTSLRVTPHVFRDRDDTRIKLL
                     VQVEDGNLTGHQVDQIPVVERSTINTQALITEGESLLIGGMVRESTSSSVDKVPGLGD
                     VPIIGNAFKNRTSSSSRVERMFLITPRLAGARPAPVGAGLRTAATAAPTSASTSAPAA
                     TSQTKPATVPVAPIAAIATPAAAPAAVAPAAPAIQTPVQRASVVLDLDAPLPAAATRS
                     ISILPAAQTSPRKP"
     gene            1052..2182
                     /locus_tag="AC16HB_06670"
     CDS             1052..2182
                     /locus_tag="AC16HB_06670"
                     /inference="COORDINATES: protein motif:HMM:NF048680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctD/MshK family protein"
                     /protein_id="HIR5606509.1"
                     /translation="MDRPALAHDLLHALELRVFEGPQTGARAPMVCGTDLVLATGPEG
                     HGIEADIVLREEGVTPARVRVTADLTDALIEVLAGEIRLGERVLTAGTQSPWAMHSPL
                     RIGSAVVAFGRAAVADWPVAIAQAEAADAAKPTATVTRARAPLHLRAEVWLAGLGATV
                     LLFCLAAFWTARSAATPLQTPDSAPPSLAAELRASEFSGLTITTGVDGHMTVHGRIGT
                     LAQRARLDAWLAEHQVAPVIDVQVDEALVRDVTETFRVNGVSVQAQVSGMGTIAVEAA
                     ERDADRLARAEEVVRRDVRGVTDLAVRNVAPPAPKPVRQVSDDPGKRIASLVPGDPAY
                     LVTADGSRYFVGSMLPTGHRITRIAAGSVTLELDGQQTTLNF"
     gene            2225..2521
                     /locus_tag="AC16HB_06675"
     CDS             2225..2521
                     /locus_tag="AC16HB_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606510.1"
                     /translation="MTTPTNLYALQSVVLTSPQPKSTGGDFFEALARAWGEALDKQAQ
                     VIQEKSAALNQDGNDTPAAITELSAESARISFLANSSHTSLSESSEALKAVAQK"
     gene            2544..2948
                     /locus_tag="AC16HB_06680"
     CDS             2544..2948
                     /locus_tag="AC16HB_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606511.1"
                     /translation="MSIEIAAASALGQAASASGATAGQVQAGYGVSMTDFGGFQQALS
                     NASARLEARPVSTPSEAAQVLSKPFDHINGEAARISAESQAVQAEGKQITPGEMVMLT
                     ARCQEFMFHCQLMSSVANKTSDGLQQLFRQQS"
     gene            2964..3824
                     /gene="sctJ"
                     /locus_tag="AC16HB_06685"
     CDS             2964..3824
                     /gene="sctJ"
                     /locus_tag="AC16HB_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HIR5606512.1"
                     /translation="MKTNTLLRRLGITANVALVLLLCACGKQELYSQLSERQANEMVA
                     VLRGAGIEAEKEVKDGQFSISTGSGDFAQAIRTLSAQGYPRETYDSMGKVFKREGFVS
                     SPLEERARLLHAMSQEISNTLTNIDGVVTARVHLNIPEKNPLADKPQPASAAVFIKYR
                     ADRNLSGQITQIKALVVNSIEGLAYDNVTVALFPAESIPAEVVKPQQSALPAAPQAIA
                     AGAPLLAGSVGGALALGGGGLLWWRRRGTAAKEPAADTRPAAGAAPERSRDIVAREMQ
                     DALRRAASRH"
     gene            3821..4402
                     /locus_tag="AC16HB_06690"
     CDS             3821..4402
                     /locus_tag="AC16HB_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606513.1"
                     /translation="MSAPDPLAHATMATNEVRERRRRRAAIAARLMAGSADGISTVGW
                     DLLDVAPDWLAWPDAGLATLQRQIGALLYAPEIRLWIDGARLGAARAALGEPYMHALA
                     AQRDLRSFPSDAPARPRIESAAQVADQLRTAGAAVLLASLPEGALRDAVAASMAPATA
                     AVMASGLAHSLINRALALGAPARSSQSTAGRQP"
     gene            4399..5031
                     /gene="sctL"
                     /locus_tag="AC16HB_06695"
     CDS             4399..5031
                     /gene="sctL"
                     /locus_tag="AC16HB_06695"
                     /inference="COORDINATES: protein motif:HMM:NF018358.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system stator protein SctL"
                     /protein_id="HIR5606514.1"
                     /translation="MSFLLWHRDRKVGVASTRVVLRASEVPLFADAQALRDRLEHLEA
                     EASQRIADAREEARASGYLQGREEGAQQARAEVAATIVELTRAAAQERARLGGEIAAL
                     ALQVVRKLLGQLPADGVLAALAETAARDMLPTQMLTLFVHPDQCEAVRARLAATSDAT
                     ADAPTPRFEVQADPACVPGACRIETEHGTVDASLEAQLARLASAWGVAVQ"
     gene            5028..>5914
                     /locus_tag="AC16HB_06700"
     CDS             5028..>5914
                     /locus_tag="AC16HB_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HIR5606515.1"
                     /translation="MTIATALQNTAETHRLLRALQTTRTVVELGRVVQATGTVLRATG
                     LRARIGQQCRIFDPATDVDGAEPLLAEVIGFAGAEVILAPYGPLQGVAVGAAVQALAD
                     EAHVPCGPALLGRVIDAFGRPLDGRPLDAAVGSTPLHASAPSPLQRRSIDAPLPTGVR
                     AIDAALTVGEGQRVGIFAMAGGGKSTLLGMLARQARADVNVIALVGERGREVREFLDD
                     SLGEDGLARSVVVVSTSDRPALERVRAAYAATAIAEGFRKEGKRVLLLVDSVTRFARG
                     LRELGLSALEPPVRRGYPPS"
BASE COUNT          927 a         2073 c         2038 g          876 t
ORIGIN      
        1 tgaacgcggt gatcgtccgc gacgtggccg agcggttgcc gcgctacgac cagctgatcg
       61 cggccctcga cgtcgagccg cagtcgctcg agatcgaagc caccatcatc gacgtcaacg
      121 tcgatcggct gcgcgaacta ggcgtcaact ggcgctggaa caacgccggc cgcgaggtcg
      181 gctacaacgg tggcgtgccg accgccggcg tcggcggcat cgcgtcggcg gttctcggct
      241 cggttggcca gttcgtcgcc cgcatccgcg cgctgcaggc cgaaggcgcg gcgcgcatcg
      301 tgtcgagtcc gcaagtggtc acgctgtcgg acgtcgaggc cgtgttcgac aacagctcga
      361 cgttctatgt gcgtgtggcc gggcgcgagc aggtggatct cttcaacgtc agcgccggca
      421 cctcgctgcg cgtgacgccg catgtctttc gcgaccgcga cgacacgcgc atcaagctgc
      481 tggtgcaggt cgaggacggc aacctgaccg gccaccaggt cgaccagatc ccggtggtcg
      541 agcgctcgac gatcaacacg caggcgctca tcacagaagg cgagagcctg ctgatcggcg
      601 gcatggtgcg cgaaagcacg tcctcgagcg tcgacaaggt tccgggcctg ggcgatgttc
      661 cgatcatcgg caatgcgttc aagaaccgga ccagcagttc gtcgcgcgtc gagcgcatgt
      721 tcctgatcac gccgcgcctg gcgggagctc gccccgctcc cgttggcgcc ggcctgcgca
      781 ccgccgcgac cgctgcgccg acgtcggcgt cgacttcggc tccggcggcg acgtcccaga
      841 cgaagccggc gaccgtgccg gtggcgccga ttgctgccat cgcgacgccg gcggcagcgc
      901 cggcggcagt tgccccggct gcgcccgcca tccagacccc cgtgcaacgc gcctccgtcg
      961 tgctcgatct cgatgcaccg ctgccggccg ccgccacgcg ctcgatctcg atcctcccgg
     1021 ccgcgcaaac ctccccgcga aagccctgac catggaccga cccgccctcg cccacgacct
     1081 gctgcatgcg ctcgagctgc gcgtattcga aggcccacaa accggtgctc gcgcgccgat
     1141 ggtctgcggc actgacttgg ttctcgcgac cgggccggaa ggccatggca tcgaagccga
     1201 catcgtgctg cgcgaggagg gcgtgacgcc ggcgcgtgtg cgggtcaccg ccgacctgac
     1261 cgatgcgctg atcgaagtcc tggcgggcga gatccgcctc ggtgaacgtg tgctgacggc
     1321 tggaacgcag tcgccgtggg cgatgcactc gccgctgcgt atcggcagcg cggtggtcgc
     1381 tttcggccgc gccgcagtgg ccgactggcc ggttgcgatt gcccaagccg aggcagcgga
     1441 cgcagcgaag ccgaccgcaa cggtcacgcg ggctcgcgcc ccgttgcacc tgcgcgccga
     1501 ggtctggctt gccggcttgg gtgccacggt gctgctgttc tgcctggccg cgttctggac
     1561 cgcgcgctcg gcggccacgc ccctgcagac cccggacagt gcgcccccct cgctcgccgc
     1621 cgagttgcgc gcctccgagt tctccggcct caccattact accggcgtcg acggtcacat
     1681 gaccgttcac ggccggatcg gcacgttggc gcagcgcgcg cgcctcgatg cctggctggc
     1741 cgagcaccag gtcgcgccgg tcattgacgt gcaggtcgac gaggcactgg tccgcgacgt
     1801 aaccgagacg ttcagggtca acggcgtctc ggtgcaggcg caggtttccg gcatgggaac
     1861 gatcgcggtg gaggccgccg agcgcgatgc cgatcgcctg gcgcgggcgg aggaggtggt
     1921 gcggcgtgac gtccgcggcg ttaccgacct tgccgttcgc aacgtggcgc ccccggcgcc
     1981 gaagccggtt cgtcaggttt ccgacgatcc gggcaagcga atcgcctcat tggtccccgg
     2041 cgacccggct tatctcgtca cggccgacgg atcgcgctac ttcgtcggct cgatgctgcc
     2101 cacgggacat cggatcaccc ggatcgcggc cggcagcgtg acgctggaac tcgacggcca
     2161 gcagacgact ttgaacttct gacttacccc ccgcaaccaa ctgcaaccgc aaaggagcaa
     2221 tcccatgacc acgcccacga acctctacgc cctgcaatcg gtcgtcctga cctcgccaca
     2281 accgaagagc accggcggcg acttcttcga agctcttgcc cgtgcgtggg gcgaggccct
     2341 cgacaagcag gcgcaggtga ttcaggagaa atccgccgca ctcaatcagg atggcaacga
     2401 cacgccggcc gcgattaccg aactgagcgc cgagtcggcg aggatcagct tcctcgccaa
     2461 cagctcgcac acatcgctgt cggagtcgtc ggaagccctc aaggcggtgg cgcagaagta
     2521 agcgaccgtt gaggtaaccc gccatgagca tcgaaatcgc agctgcctcg gcgcttggcc
     2581 aggccgcttc cgcctccggc gcgacggctg gccaggtgca agccggttat ggcgtgtcga
     2641 tgaccgactt cggaggtttc cagcaggcgc tttcgaacgc gagcgcccgc ttggaagccc
     2701 ggccggtcag cacgccctcc gaggccgcac aggtgctgtc caagccattc gatcacatca
     2761 acggcgaggc ggcgcgaatc tctgccgaaa gccaggccgt gcaggccgaa ggcaagcaga
     2821 tcacgccggg tgagatggtg atgctgaccg cacgctgcca ggaattcatg ttccattgcc
     2881 agctgatgtc cagcgtcgcc aacaagacgt cggacggcct gcagcagctg ttccgccaac
     2941 aatcctgacc cacaaggacg accatgaaga cgaatacgct cctgcgacgc ctgggcatca
     3001 ccgcgaacgt tgcgctggtg cttctgctgt gtgcctgcgg caagcaggag ctctattcgc
     3061 aactgtccga acgccaggct aacgagatgg tggcggtgct gcgcggcgcc ggcatcgagg
     3121 ccgagaagga agtcaaggac ggacagttct cgatctcgac cggcagcggc gacttcgcgc
     3181 aggcgatccg caccctgagc gcccagggct atccgcgcga gacctacgac agcatgggca
     3241 aggtcttcaa gcgggaaggc ttcgtctcct cgccgctgga agagcgtgcg cgactgctgc
     3301 atgcgatgtc gcaggaaatc tccaacacgc tcaccaacat cgacggcgtg gtcaccgcgc
     3361 gcgtgcatct gaacattcca gagaagaacc cgctcgcaga caagccgcag ccggcctcgg
     3421 cggccgtatt catcaagtac cgggccgacc gcaacctttc gggtcagatc acccagatca
     3481 aggcgctggt cgtcaacagc atcgaagggt tggcctatga caacgtcacg gttgcgctgt
     3541 ttcccgccga gagcatcccg gccgaagtcg tgaagccgca gcagtcggcg ctgccggcgg
     3601 ctccccaggc aatcgccgca ggagcgcctc tgctggccgg ctccgtcggc ggcgcgctcg
     3661 cgctgggcgg cggcggcctg ctctggtggc gccggcgcgg cacggcagcg aaagaaccgg
     3721 ccgcggatac gcgtccggcg gctggcgcgg caccggaaag atctcgcgac atcgtcgcgc
     3781 gcgaaatgca ggacgcactg cgccgcgcgg cgagccgcca ttgagcgcgc cggacccgct
     3841 cgctcacgcc accatggcga ccaacgaggt gcgcgaacgc cgccggcgcc gggccgcgat
     3901 tgcggcccgg ctgatggccg gcagcgccga cggaatctcg acggtgggct gggacctgct
     3961 cgacgtcgcg cccgactggc tggcctggcc cgacgcagga ctcgcgacac tgcagcggca
     4021 gatcggcgcg ctgctgtacg cgcccgaaat ccgtctctgg atcgacggtg cccgtctagg
     4081 cgccgcgcgc gccgcgctcg gggaacccta catgcatgcg ctggcggcgc aacgcgacct
     4141 gcggtcgttc ccgagcgatg cgcctgcgcg cccgcgcatc gagtcagccg cgcaagtggc
     4201 agatcaactt cggaccgccg gcgcggcggt acttctcgcg tcgctgccgg aaggcgcatt
     4261 gcgggatgcg gttgccgcgt ccatggctcc ggccaccgcg gccgtgatgg cgagcggact
     4321 ggcgcattcg ctgatcaacc gcgcactggc gctcggcgcg ccggcgcgat cgtcacagtc
     4381 gaccgccggg aggcaaccgt gagctttctg ctctggcatc gcgaccgcaa ggtcggcgtc
     4441 gcgtccacac gcgtcgtgtt gcgcgcgagc gaagtgccgc ttttcgccga cgcgcaggcg
     4501 ctgcgcgacc ggctcgagca tctggaggcc gaagcgtcac agcgcatcgc cgacgcccgt
     4561 gaagaggcgc gcgcgagcgg atacctgcaa gggcgcgagg agggcgcgca gcaggcacgc
     4621 gccgaggtcg ccgcaacgat cgtcgagctg accagggcgg ccgcgcagga acgcgcgcgc
     4681 ctgggcggcg aaattgctgc gctcgcgctg caggtggtgc gcaagctgct ggggcagctt
     4741 cccgccgacg gcgtgctggc agcgctggcc gaaaccgctg cgcgcgacat gttgccgaca
     4801 cagatgctga ctctcttcgt tcacccggac cagtgcgagg ccgtgcgcgc gcggctggcc
     4861 gcaacatccg atgcgacggc cgatgcgccg acgccgcgct tcgaggtgca agccgatccg
     4921 gcctgtgtgc ccggcgcctg ccgcatcgaa accgagcacg gaacggtcga tgcatcgctg
     4981 gaggcgcagc tcgctcggct cgcgagcgcc tggggagtgg ctgtgcaatg acgatcgcta
     5041 ccgccctgca gaacactgcc gaaacgcatc gccttctgcg cgcgctgcag accacgcgaa
     5101 cggtggtcga actcggtcgc gtcgtgcaag ccaccggcac ggtgctgcgc gccaccggcc
     5161 tgcgtgcgcg gatcggccag caatgccgga tcttcgatcc ggcgaccgac gtcgacggcg
     5221 ccgagccgct gctggccgaa gtgatcgggt ttgccggcgc cgaagtgatt ctcgcgccct
     5281 acgggccgct gcagggcgtg gccgtcggcg ctgccgtgca ggcgctcgcc gacgaagcgc
     5341 atgtgccgtg cggacccgcg ctgctgggcc gcgtgatcga cgctttcggc cgtccgctcg
     5401 atgggcggcc attggacgcc gcggtcggca gtacgcccct gcatgcgagc gcgccgtcgc
     5461 cgctgcaacg acgcagcatc gatgcgccac tgccgaccgg tgtgcgcgcg atcgatgcgg
     5521 cgctcaccgt cggcgaagga cagcgcgtcg gcatctttgc gatggccggc ggcggcaagt
     5581 cgacgctgct cggcatgctg gcgcggcagg cgcgcgccga cgtcaacgtg atcgcactgg
     5641 tcggcgaacg cggccgcgaa gtgcgcgaat tcctcgacga cagcctgggc gaggacggct
     5701 tggcgcgctc ggtggtagtg gtatcgacct ccgaccggcc ggcgctcgaa cgggtgcgtg
     5761 cggcctatgc cgccaccgcg atcgccgaag ggttccgcaa ggaaggcaag cgcgtgctgc
     5821 tgctggtcga ctcggtcacg cgctttgcgc gtggcctgcg ggagctcggc ctgtcggcac
     5881 tcgagccgcc ggtgcggcgc ggctatccgc cctc
//
LOCUS       DBNHBY010000272         4229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_557347, whole genome shotgun sequence.
ACCESSION   DBNHBY010000272 DBNHBY010000000
VERSION     DBNHBY010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_557347"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..368
                     /locus_tag="AC16HB_06705"
     CDS             141..368
                     /locus_tag="AC16HB_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HIR5606516.1"
                     /translation="MIVIDARGLEPPAPFERVMEALCDLQPDQRILLILEREPFPLYR
                     VLERNGYAHRTRQSDQGYFEVEIGAPEIFRS"
     gene            complement(365..1063)
                     /locus_tag="AC16HB_06710"
     CDS             complement(365..1063)
                     /locus_tag="AC16HB_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5606517.1"
                     /translation="MATTPISAETFLAAMPLFRELAPAQRERIAERTRMLRVARGDTL
                     FHRGDPCLGMHLIVHGQVKLAFVSSSGVEKVIEFLGAGQTFGEAVMFLDAPHVVTAQA
                     LADSLLLFIPKEAVFERIESDAGFARGMLAGLARRLHQLVADVEAFSTRSGTERVIGF
                     LLRDCATAGEEIECGPVDVVLPVAKGVIASRLNLTQEHFSRILHDLSATGLIEVRGRN
                     IRVPDPRRLRDYAP"
     gene            complement(1436..1894)
                     /locus_tag="AC16HB_06715"
     CDS             complement(1436..1894)
                     /locus_tag="AC16HB_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5606518.1"
                     /translation="MRARLAGANVFNQPVKSIMERHKLLLAAPTTTVLAAAKMMADKK
                     VGAIVVVDDDRLVGIFTERDALFRVIARGLAPSTTVLGDVMTREPRTIAPGKTFGHAM
                     LVMHEGAFRHLPVVESGKLIGIVSARDALDPDLEEFVFEERRRRQLQETR"
     gene            2049..2621
                     /locus_tag="AC16HB_06720"
     CDS             2049..2621
                     /locus_tag="AC16HB_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5606519.1"
                     /translation="MTRVLVDFSTPAAGFDQPLELWGACHGRVRRMIGLLQRLAEHVP
                     KAGANSDARTTATSIRRYFDEAAPRHHDDEEIDLFVRLRERLGQLDAAHAKQVADAIA
                     TLESDHHDLSALWRTLREALVCIEGGQAAVLDEATIAVFVNRYEAHMELEETVIEPAL
                     KRLLKKSDLAAIGQSMAKRRGVDWSELAAR"
     gene            complement(2618..4138)
                     /locus_tag="AC16HB_06725"
     CDS             complement(2618..4138)
                     /locus_tag="AC16HB_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR5606520.1"
                     /translation="MADPLIIAKAGTRELALLPALANRHGCITGATGTGKTVTLQKLA
                     ESFSRIGVPVFMADVKGDLTGICKPGTLTDKLKARFEMLKVDAPKWEGCPVTLWDVFG
                     EQGHPVRATISDMGPLLLARLLNLNETQEGVLALVFKVADDNSLLLLDLKDLRSLLQF
                     VGDNAEQFKTQYGNVSSASIGAIQRGLLQIEQQGGDRFFGEPMLNVDDLMQTVEGRGV
                     ISVLAADKLLQSPKLYSTFLLWLLSELYERLPEVGDRDKPKLVFFFDEAHLLFDEAPV
                     ALLDKVEQVVRLIRSKGVGVYFVTQNPLDIPDKVLGQLGNRVQHALRAFTPRDQKAVA
                     TAAGTMRPNPKLDIGKAITELAVGEALISFLDPKGSPTVTERAFVLPPASQIGPIAPD
                     ERKQLIAGSIVAGQYEKTVDRESAFEKLKARAGTGQPLPGSTEPPGGATAPAQPGGGL
                     LGGLGDILFGSTGPRCGRREGMVDSMAKSAARSVGSAVAREVMCGVLGSLLGGRWR"
BASE COUNT          690 a         1452 c         1414 g          673 t
ORIGIN      
        1 accgcggcgg cgggaatggc gctgtacaca ttcgacagtc cgctgtggct ggcggttctg
       61 cacgcggccc tggccggcgg gctggtggcc gcgctcgcgg tcctcgccgc cggatgcaag
      121 gcttcgatgg agagtgcctg atgatcgtga tcgacgcgcg cggactcgag ccgccggccc
      181 cgttcgagcg ggtgatggaa gcgctatgcg acctgcagcc ggaccagcgc atcctgctga
      241 tcctcgagcg cgagccgttt ccgctgtatc gcgtgctcga gcgcaacggt tatgcgcacc
      301 ggacacggca gtcggatcag ggctacttcg aggtcgagat cggcgcgccc gagatcttca
      361 gaagctaggg cgcatagtcg cgcaggcggc gcggatcggg cacgcgaata ttgcggccgc
      421 gcacctcgat cagaccggtg gccgacaggt cgtgcaggat gcgcgagaag tgttcctgcg
      481 tcagattgag ccgcgacgcg atcacgccct tggcgaccgg cagcaccacg tcgacggggc
      541 cgcattcgat ttcctcgccc gcggtcgcgc agtcgcgcag cagaaagccg atcacgcgtt
      601 cggtgccgga tcgggtcgag aacgcctcga cgtcggccac cagctgatgc aggcgtcgcg
      661 ccagcccggc cagcatgccg cgcgcgaagc cggcgtccga ctcgatgcgc tcgaacaccg
      721 cctccttggg gatgaacagc agaagcgagt ctgcgagcgc ctgcgcggtc accacgtgcg
      781 gcgcgtcgag gaacatcacc gcctcgccga aggtctgccc ggcgccgagg aactcgatca
      841 ccttctccac gccggaggac gatacgaacg ccagcttgac ctggccgtga acgatcaggt
      901 gcatgccgag gcacgggtcg ccgcgatgga acagcgtgtc accgcgcgcc acgcgcagca
      961 tgcgcgtgcg ctccgcgatg cgctcgcgct gcgcgggcgc cagctcgcgg aacagcggca
     1021 tcgcggcgag gaacgtctcg gcactgatcg gagtcgtggc catcgttgcg gttcagaacg
     1081 cggaatcggg cgaagtctcg tgcgcgcgcg ccggcgaaat ttgatttcgg tcagaccgaa
     1141 gggagcagcg gcgcgcgact gcctcgttcg cgccgcgccg gcaccacttt cggagtggcc
     1201 ccaacgcccc gcacgtcgcg ggtgcgacgt tgtgccatgt caatcacgcg cggcacgcac
     1261 gcccctaacg tacgcggacc acagtttacc tgcccgcatc acgatggccc gccgcgatcg
     1321 agctgccgga cattgccgcc aaggtgcgcg tcgagggcga cgcgatcgaa gtgacgaccg
     1381 ccgccggtgc gctgccgctg cgcgcagtcg acctgcggct cgacggagac cgcttctagc
     1441 gcgtctcctg cagttgccgg cggcggcgct cctcgaacac gaattcctcc aggtccggat
     1501 cgagcgcatc gcgcgcggac acgatgccga tcagtttgcc gctttcgacc accggcaggt
     1561 ggcgaaaggc gccttcgtgc atcaccagca tcgcatgccc gaacgtcttg ccgggcgcga
     1621 tcgtcctcgg ctcgcgcgtc atcacgtcac cgagcaccgt ggtcgacggc gcgagtccgc
     1681 gcgcgatcac ccggaacagc gcatcgcgct cggtgaagat gccgaccagg cgatcgtcgt
     1741 cgaccacgac gatggcgccg accttcttgt ccgccatcat cttcgcggct gcaagcaccg
     1801 tcgtcgtcgg cgcggcaagc aacagcttgt gccgctccat gatgctcttt acaggctgat
     1861 tgaacacgtt ggccccggcc aatcgggcgc gcatgcgcgc agtggggcat taatagcgag
     1921 gtgagcggcc catgcgcttg acgtaaggca aatttctctc gatggtctac cgcaaagcgc
     1981 tcggccacgc gactgaagcg ccgatatcat tcccggcacc aacgatgttc ggccgtggag
     2041 acttggcagt gacgcgcgta ctcgtcgatt tcagcacccc ggcggcgggg ttcgatcagc
     2101 cgctcgagct ctggggcgcg tgccacggcc gcgtgcgccg gatgatcggc ctgctgcagc
     2161 ggctcgccga gcacgtgccc aaggcgggcg cgaacagcga cgcgcgcacg acggcgacct
     2221 cgatccggcg ctatttcgac gaggccgcgc cgcgtcatca cgacgacgag gagatcgatc
     2281 tcttcgtgcg gctgcgcgaa cgcctcggcc agctcgatgc agcgcacgcg aagcaggtgg
     2341 ccgacgcgat tgccacgctg gaatccgacc accacgatct gtcggcgctg tggcggacgc
     2401 tgcgcgaagc gctggtctgc atcgagggcg ggcaggcggc cgtgctcgat gaagcgacga
     2461 tcgcggtctt cgtcaaccgc tacgaggcgc acatggagct cgaggaaacg gtgatcgagc
     2521 cggcactcaa gcgcctgctg aagaagagcg atctggccgc gatcggccag tcgatggcaa
     2581 agcggcgcgg cgtggactgg agcgaactcg cggcgcgcta gcgccagcgc ccgccgagca
     2641 gcgaaccgag cacgccgcac atgacctcgc gcgccaccgc cgagccgacg ctgcgcgccg
     2701 cactcttggc catcgaatcg accatgcctt cgcggcggcc gcagcgcgga ccggtcgagc
     2761 cgaacaggat gtcgcccagc ccgcccagga ggccgccgcc cggctgtgcc ggcgcggtcg
     2821 cgccgcctgg cggttccgtg ctgcccggca gcggctgacc cgtgccggcg cgcgccttca
     2881 gcttctcgaa ggcgctttcg cgatcgaccg tcttttcgta ctggccggcg acgatcgagc
     2941 ccgcgatcag ctgcttgcgc tcgtccggcg cgatcgggcc gatctggctc gccggcggca
     3001 gcacgaacgc gcgctcggtg acggtgggcg agcccttcgg atcgagaaac gagatcagcg
     3061 cctcgccgac cgccaactcg gtgattgcct tgccgatgtc cagcttcggg ttcggccgca
     3121 tcgtgccggc ggcggtcgcc accgccttct ggtcgcgcgg ggtgaacgcg cgcagcgcgt
     3181 gctgcacgcg gttgccgagc tgaccgagca ccttgtccgg gatgtcgagt gggttctgcg
     3241 tgacgaagta gacgcccacg cctttcgagc ggatcagccg caccacctgc tcgaccttgt
     3301 ccagcagcgc gaccggcgcc tcgtcgaaca gcagatgcgc ctcgtcgaag aagaacacca
     3361 gcttcggctt gtcgcggtcg ccgacctccg gcagccgttc gtacagctcg gacagcagcc
     3421 acagcaggaa tgtcgaatac agcttgggcg actgcagcag tttgtcggcg gcgagcacgc
     3481 taatcactcc ccggccctcg accgtctgca tcaggtcgtc gacgttcagc atcggctcgc
     3541 cgaagaaccg gtcgcccccc tgctgctcga tctgcaacag gccgcgctgg atcgcgccga
     3601 tcgatgccga cgacacgttg ccgtactggg tcttgaactg ctcggcgttg tcgccgacga
     3661 actgcagcag cgagcggaga tccttcaggt ccagcagcag caggctgttg tcgtcggcga
     3721 ccttgaaaac cagcgcgagt acgccttcct gcgtctcgtt caggttcagc aggcgcgcca
     3781 gcagcagcgg ccccatatcg gagatcgtcg cgcgtaccgg gtgcccctgc tcgccgaaca
     3841 cgtcccacaa cgtgaccggg cagccttccc atttcggcgc atcgaccttc agcatctcga
     3901 accgcgcctt cagcttgtcg gtcagcgtgc ccggcttgca gatgccggtc aaatccccct
     3961 tcacgtcggc catgaacacc ggcacgccga tcctcgagaa actctccgcc agtttctgca
     4021 gcgtcacggt ctttccggtg ccggttgcgc cggtgatgca gccgtgccgg ttggcaagcg
     4081 ccggcagcag cgcgagttcg cgggttccgg ctttggcgat gatcaggggg tcggccatgt
     4141 gaacctcgga ttgcggatcg cagatgaagg cttgctctga agcgtagcgc atgtgggccg
     4201 acccaagaac ggcgattgat agaattcgc
//
LOCUS       DBNHBY010000273         3988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1436940, whole genome shotgun sequence.
ACCESSION   DBNHBY010000273 DBNHBY010000000
VERSION     DBNHBY010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1436940"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16HB_06730"
     CDS             <1..266
                     /locus_tag="AC16HB_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HIR5606521.1"
                     /translation="VLDTVQLGCTGPNQMKSFLRCGMGPCQGRLCGLTVTELIARARN
                     VAPGAVGHYRLRFPVKPITLGELASLPQTPESVQAVVRIGKSH"
     gene            270..1388
                     /locus_tag="AC16HB_06735"
     CDS             270..1388
                     /locus_tag="AC16HB_06735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606522.1"
                     /translation="MNPVASADVIVIGGGLHGCSAALHLARAGAATIVLEKNYVGRHA
                     SGVNAGGVRTLSRHEAEVPLALAALELWYRIGELVDDDCGFETHGQIRVAENEDDVAQ
                     LRQREARMRTLGYTHERWIDRDELFARVPALARHCLGGLIAERDGAAAPYQTTAAFRR
                     KAEALGQRFFEGVRVGRVQRASNAWTVETDQGRFAARSLVNCAGAWADRICAQLDEPV
                     PLEAIAPMMMVTLRMPRFLEPVVLGTGRPLSFKQTCEDTVLIGGGRRARVERDAESTE
                     LDFRELAASAQTVWDLFPIMRRATIHRGWAGIEARMPDDIPVIGPSQTTPDAYHAFGF
                     SAHGFELGPIVGRITADLITQGTTSLPIAPFSIARFRR"
     gene            1461..2672
                     /locus_tag="AC16HB_06740"
     CDS             1461..2672
                     /locus_tag="AC16HB_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606523.1"
                     /translation="MNDRRLIYAAGLLRGLAAGAISVLAAVYLAKRGFPETEIGIVLS
                     AGLAGVLAGTVYGTFCADRVGRRRSLVLLALLAAAGGCALAWADSLWAASAAAFCGMV
                     SAQGTDRGAAQALEQAILPGIVTARERTRVFAWYNALQDAGGAAGALLAALPAVLRDG
                     LALPESRAYDATIFAYAALLVAVGLLYARLSHAAESNATAANHDANFLPPSAKRDVYR
                     FSALSALDSIGGGFIGSALIAYYLYARFGVGEGALALLFTAARMMNVFSHFAAAWIAA
                     RIGLVNTMVFTHIPSSLLLLTLPIAPSFPVAALLFVLREGLSEMDVPTRQSYLMAIVP
                     EHERSWAAGLSQLARAAGRMIAPAFAGAAMQAGALWLPLVAGATIKIGYDLLLWRAFR
                     RVRPPEEAAPT"
     gene            2741..3163
                     /gene="soxX"
                     /locus_tag="AC16HB_06745"
     CDS             2741..3163
                     /gene="soxX"
                     /locus_tag="AC16HB_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR5606524.1"
                     /translation="MKRALSIALTLLSGIASAQVVDYKVAGDGIPKPLTATPGNAARG
                     KALLAQREAANCLTCHSINDKDLKPGGKRGPALDGIGAQLTASQLRLSVVDYTRISRG
                     TDMPTFHKAARGSDNDQPILSAQEIEDVVAFLTTLRYK"
     gene            3276..>3988
                     /locus_tag="AC16HB_06750"
     CDS             3276..>3988
                     /locus_tag="AC16HB_06750"
                     /inference="COORDINATES: protein motif:HMM:NF022665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HIR5606525.1"
                     /translation="MEEQRSYLAWVIGIAVLVAAAAIGYNAWRQSRPVEVPPPVAQSE
                     MPAPPKAPAEPQIQHPLPANEAEPLPPLEKSDALLAESLTQLVGGKSVAELLVTDGIV
                     RRIVATIDNLPREKVATRIMLARPVPGTLAVDKSGDDMLLAARNSARYARYVQLAQSI
                     DVKRAVALYVRLYPLFQRAYQDLGYPKGYFNDRLVEVIDHLMAAPEARAPLKLMQPKV
                     RYEYVDPALESLSAGQKIML"
BASE COUNT          593 a         1367 c         1410 g          618 t
ORIGIN      
        1 aggtgctcga cacggtccag ctcgggtgca ccgggccgaa ccagatgaag agtttcctgc
       61 gctgtggcat gggaccgtgc cagggccggc tgtgcgggct gaccgtgacc gagctgatcg
      121 cgcgcgcacg caacgttgcg cctggcgccg tcggtcacta ccggctgcgc tttccggtca
      181 agccgatcac gctcggcgaa ctggcgtcgc tgccgcagac gccagagtcg gtgcaggccg
      241 tggtgcggat cggcaagtcg cactgagtcg tgaatccggt cgcgagcgcc gacgtgatcg
      301 tgatcggcgg cggcctgcat ggctgctcgg ccgcgctgca cctggcgcgc gcgggtgccg
      361 cgacgatcgt gctcgagaag aactacgtcg ggcgtcacgc ctcgggcgtc aatgccggcg
      421 gcgtgcgcac gctgtcgcgg cacgaggccg aggtgccgct tgcgctcgct gcactcgagc
      481 tgtggtaccg catcggcgaa ctcgtcgacg acgattgcgg cttcgagacc cacggccaga
      541 tccgcgtggc ggagaacgag gacgatgtcg cgcagctgcg gcagcgcgag gcgcgcatgc
      601 gcacgctcgg ctacacgcac gagcgctgga tcgaccgcga cgaactgttc gcgcgcgtgc
      661 cggcgctggc gcgccactgt ctcggcggac tcattgccga acgcgacggc gccgcggctc
      721 cgtaccagac caccgccgca ttccgccgca aggccgaagc gctcggccag cgcttcttcg
      781 aaggcgtgcg cgtcgggcgt gtgcagcgcg cgagcaacgc atggaccgtc gagacggatc
      841 aaggccgctt cgccgcgcgc agtctggtca actgcgcggg cgcgtgggcc gatcgcatct
      901 gcgcgcaact ggacgagccg gtgccgctgg aggcaatcgc gccgatgatg atggtcacgc
      961 tgcgcatgcc gcgcttcctc gagcccgtcg tgctcggcac cggccggccg ttgtcattca
     1021 agcagacctg cgaggacacg gtgctgatcg gcggcgggcg gcgcgcgcgg gtggagcgcg
     1081 atgcggagtc gaccgagctc gacttccgcg aactggctgc ctccgcgcag acggtgtggg
     1141 acctgtttcc gatcatgcgg cgggcaacga ttcatcgcgg ctgggccggc atcgaggcgc
     1201 ggatgcccga cgacattccg gtgatcgggc ccagccagac gacgcccgac gcgtaccacg
     1261 ccttcggttt ctcggcgcac ggcttcgagc tcgggccgat cgtcggacgc atcacggccg
     1321 acctgatcac tcaaggcacg acgagtctgc cgattgcacc gttctcgatt gcgcgctttc
     1381 gccgatgagc caatgccgat ctgccccaac gtggttcgtt ccttcttgga gcgcaccgcc
     1441 gcaggcatga agggggccgc ttgaacgatc gccggctcat ctatgcggcc ggactgctgc
     1501 gtggcctggc ggccggcgcg atcagtgtgc tggcggccgt atacctcgcc aagcgcggct
     1561 tcccggaaac cgaaatcggc atcgtgttga gcgccggtct cgcgggcgtg ctggccggca
     1621 ccgtgtacgg cacgttctgc gccgatcgcg tcggccggcg ccgcagcctc gtgctgctcg
     1681 cgctgctggc tgccgccggt ggctgtgcgc tcgcttgggc ggactcgctg tgggccgcga
     1741 gcgccgcggc gttctgcggc atggtgagcg cgcagggtac cgatcgcgga gcggcacagg
     1801 cgctggagca ggcgatcctg cccggcatcg tgacggcgcg ggagcgcact cgggtgttcg
     1861 cctggtacaa cgcgctgcag gacgcgggcg gcgccgcggg cgcgctgctc gcggcgttgc
     1921 ccgccgtgct gcgcgacgga ctcgcgctgc ccgagagccg cgcctatgac gcgacgatct
     1981 tcgcgtacgc cgcgctgctt gtcgcggtcg ggttgctgta tgcgcggctg tcgcacgcgg
     2041 ccgagtcgaa cgccaccgcg gcgaatcacg atgcaaattt cctgccgccg agtgcgaagc
     2101 gcgacgtcta tcgcttctcg gcgctgtccg cgctcgattc catcggcggc ggtttcatcg
     2161 gctcggccct gatcgcctat tacctgtacg cgcgcttcgg cgtcggcgag ggcgcgctcg
     2221 cgctgctgtt caccgccgcc cgcatgatga acgtgttctc gcacttcgcg gcggcgtgga
     2281 tcgccgcgag gatcggactg gtcaacacga tggtgttcac ccacattccg tcgagcctgt
     2341 tgctgctgac gctgccgatc gcgccgtcgt ttccggtagc ggcgctgctg ttcgtcctgc
     2401 gggagggcct gtcggagatg gacgtgccga cgcggcagtc gtacctgatg gcgatcgtgc
     2461 cggagcacga gcgctcgtgg gccgcggggc tgtcgcaact ggcgcgcgcg gcgggacgca
     2521 tgatcgcgcc ggcattcgcg ggtgcggcga tgcaggcggg cgcgctgtgg ctgccgctgg
     2581 tggccggcgc aacgatcaag atcggctacg acctgctgct gtggcgcgcg tttcgccgcg
     2641 tgcggccgcc ggaggaagcc gcgccgactt gagctacgcc aacacccgaa acgaccgttc
     2701 gggcacagtg aatgcggcat ctgacacgag cagcaccgcc atgaaacgcg cactctcgat
     2761 cgcactcaca ctcctcagcg gcatcgcatc ggcgcaggtg gtcgactaca aggtcgcggg
     2821 cgacggcatt ccgaaaccgc tgaccgccac gcccggcaac gccgcgcgcg gcaaggcgct
     2881 gctcgcgcag cgagaggccg ccaactgcct gacctgccac tcgatcaacg acaaggacct
     2941 caagccgggc ggcaagcgcg gaccggcgct cgacggcatc ggcgcgcagc tcaccgcctc
     3001 gcagttgcgg ctctccgtcg tcgactacac gcgaatctcg cgcggcaccg acatgccgac
     3061 cttccacaag gccgcccgcg gctccgacaa cgaccagccg atcctgagcg cgcaggaaat
     3121 cgaggacgtg gtcgcgttcc tcaccacgtt gcggtacaag tagccggctt gccccacgat
     3181 tgcgggaccg aggtgtcccg tgttgacgcg cgccggaacg attccgctcg ccatcggacc
     3241 aaaatctctg cgagaagccg cgcagagggg gcaccatgga agagcaacgc agttatctcg
     3301 cctgggtgat cggcatcgcc gtgctggtcg cggcggcggc gatcggctac aacgcgtggc
     3361 ggcagtcgcg cccggtcgaa gtgccgccgc cggtcgcgca gagcgagatg cccgcgccgc
     3421 ccaaggcgcc ggccgagccg cagattcagc acccgttgcc cgcgaacgaa gccgagccgc
     3481 tgccgccgct ggaaaagagc gacgcactgc tggccgaatc actgacgcag ctggtgggcg
     3541 gcaagagcgt cgccgaactc ttagtcaccg acggcatcgt ccgccgcatc gtcgcgacga
     3601 tcgacaatct gccgcgcgag aaggtggcca cgcgcatcat gctggcgcgg ccggtgcccg
     3661 gcacgctggc ggtcgacaag agcggcgacg acatgctgct cgccgcgcgc aactccgcgc
     3721 gctacgcgcg ctatgtgcag ctcgcgcagt cgatcgatgt caagcgcgcg gtggcgctgt
     3781 acgtcaggtt gtacccgctg ttccagcgcg cgtaccagga cctcggctat ccgaagggct
     3841 acttcaacga ccggctggtc gaggtgatcg accacctcat ggcggcgccc gaggcgcgcg
     3901 cgccgctgaa gctgatgcag ccgaaggtgc gctacgagta cgtcgacccg gcgctggagt
     3961 cgctgtcggc gggacagaag atcatgct
//
LOCUS       DBNHBY010000274        10216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1166784, whole genome shotgun sequence.
ACCESSION   DBNHBY010000274 DBNHBY010000000
VERSION     DBNHBY010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1166784"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="AC16HB_06755"
     CDS             complement(<1..1882)
                     /locus_tag="AC16HB_06755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5606526.1"
                     /translation="MTIESSARREFLQTAALVVGFSFAGAPVVRAQPSAGAGTHTLDP
                     TEVDAFLAVRKDGTVVIYTGKVDLCTGHRIAMRQMVAEELNMDADDLARIELVEGDTW
                     LTPDQGPTAGSTGVMRGGVQLRQAAATAREALLALASSRLKRPAGEPTLDAGLVGAAD
                     GGPKVTIAELAGARPFNVKLNPKAALKDPLRYSLVGRSLPRPDLPAKLTGRHVFVHDF
                     RLANMLYGRVLRPATVGAKLLEVDESSIGHLRGARVVRIENFLGVVANDEWTAVRAAR
                     ELKAKWSAGRGLVGDAGVRDWAKRGPFVKDDVLLSKGSTAALGADTRALRATFFWPVQ
                     SHASMGPSCAVADVRADSATIWSASQATHRYQPALAHVAGLPRDKVRVIYLDGAGCYG
                     MNGHDDAAADAVLLSKAVGRPVRVQWMREDELGWDPKGPPQLIELRALLNDDNRIAAW
                     EAELWVPMATANLSHVPLLGPTDAGIAQPAGQSVGLISQNADPPYAVANVKVTAHWLA
                     DAPLRPSNIRAPGKVANCFAVECFIDQLAASAKLDPVEFRLRDLANPRGVEVLQRCAA
                     LIGWTPRPSPAGQSGGKARGRGIAYIHYKHNETFVAVAMEVEVDRASGAIRVLQVACA
                     HDC"
     gene            complement(1884..2354)
                     /locus_tag="AC16HB_06760"
     CDS             complement(1884..2354)
                     /locus_tag="AC16HB_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5606527.1"
                     /translation="MPAFELIVNNLRQRVESWDADQPLLYVLRNDLGLHGAKFGCGVG
                     QCGSCTVLIDDRPARACVTPVQAVAGKRITTIEGLGTPDKPHPVQAAFIAGQAAQCGY
                     CTTGVVMSTVALLRSNPRPTREQAKAALDGNLCRCGSHDRVLRAVLRAADAGRT"
     gene            2467..3378
                     /locus_tag="AC16HB_06765"
     CDS             2467..3378
                     /locus_tag="AC16HB_06765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR5606528.1"
                     /translation="MAARLFQGVKGVAATLVLVLNTLVFVTAMVPFALVKLIAPAAPV
                     RRVVDRVLNALASYWIRVNGAWIAAVGHPRWDLQGVEGLKRRGWYLIASNHTSWVDIL
                     VLQKVFSGRIPMLKFFLKQELIWVPVIGLAWWALDFPFLKRGRGGASGQVDLESTRRA
                     CERFKLIPTSVIIFVEGTRFTHAKHDAQRSPYAHLLKPKGGGLAIALATMGGQFDALL
                     DVTLAYPDGTPTFWDLLSGRVHEVAVRVRQRPIPPALHDASAKRTQVQEWIDAIWRDK
                     DRLLDELLSRASATSSSSSGGAERSCS"
     gene            complement(3317..4069)
                     /locus_tag="AC16HB_06770"
     CDS             complement(3317..4069)
                     /locus_tag="AC16HB_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5606529.1"
                     /translation="MVAAAVAREPPARNTRYAAIFRVLADGIRSGRYPVGGRLPTELE
                     LCKTFRASRHTVREAIRRLTDQGLIARRPGAGTVVLRRTRSGGFTQRISALQDLLAYV
                     KTAQLEVLEARDIKASPAEARLLKCRRGQAWHRLTALKHLRGARHPVAYVLAYVHRDH
                     PALRGVLDRRGVGLHDFIEDKIGEPIVTVEQEFSAKPLVGHEAEALGVEAGYAGFVIG
                     RRYISASGKSVLVTSTIFPHHRMNYSMSLKLG"
     gene            4219..5256
                     /locus_tag="AC16HB_06775"
     CDS             4219..5256
                     /locus_tag="AC16HB_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606530.1"
                     /translation="MKLRCRASCVALALTAAFASAAALAQGKLTVAAYGGSFEDMMRK
                     EIMPAFEKKTGAKLEYVAGNSTDNIARMQAQRANMEVDVAIVDDGPMYQAVALGLCGE
                     LKGVNFGELYPVADMKTGKSVTIGVVATGLMYNKKYFDDNKMAAPTSWNDIKDQKYRK
                     KLVIPPLNNTYGLQTVIMMARLAGGGEKNIDPGFKVMKDEVAPNVLAFEPQPGKMTEL
                     FQSGQAIIAVWGSGRVKAFQDTGFPAEFVYPKEGAMALGISACPTAKSNPSPLAMQFI
                     QFLLQPDTQAVFAKGAGAGPVNRKTVLPPELQKGLPYGDAVGKLLTTDWDTVNANRTA
                     WNNRFTREIER"
     gene            5281..6321
                     /locus_tag="AC16HB_06780"
     CDS             5281..6321
                     /locus_tag="AC16HB_06780"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606531.1"
                     /translation="MSFLVLEGLTKRYGDHAAVDGISLAVAKGEFVSLLGPSGCGKTT
                     TLQMIAGFVEPTAGRIALEGRDLLVVPAAKRGLGIVFQNYALFPHMTVVENVAFGLEM
                     RNVGRTERQQRVNETLALVGLSGFAERHPAQLSGGQQQRVALARALVIRPELLLLDEP
                     LSNLDAKLREEMQVELRRIQRAVGITTLMVTHDQAEALALSDRVVVMHQGRIEQQDSP
                     LAAYEHPRTGFVCDFLGKANLFTVERVDADAMKIGVLTLPLPAGDPVRAGDRILVRPE
                     KISFGAPGPGALAARVTNRVFQGNHWLYQLATPLGDAQVIRQNDGGACPVEGDEVHLV
                     WRADAMRAVAAH"
     gene            6330..7202
                     /locus_tag="AC16HB_06785"
     CDS             6330..7202
                     /locus_tag="AC16HB_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606532.1"
                     /translation="MAAGKRIAAYWLSAPALAVYAIFLVVPIASVALISFQSFEFYGG
                     IQPGFTLKNYREVFTDSYYWEVFGRTFWIAAVVTALSAVLGTFEAIVLHKMANPWKSF
                     FLLIVLGPLLISVVVRTLGWALLFGSTGLVNQALLALGLIQEPIAFMYSVTGVVIALT
                     HVLVPFVVISVWASLQRIDPAVERAAVSLGAGQPTVVRRVIVPLAMPGILSGSVIVFA
                     LAASAFATPAIIGGRRLKVIATAAYDEFLNTLNWPLGASLAVLLLAANVLVLYASNRM
                     IERRYKAVFRESAA"
     gene            7199..8002
                     /locus_tag="AC16HB_06790"
     CDS             7199..8002
                     /locus_tag="AC16HB_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606533.1"
                     /translation="MIRNGALALAFHALFIGFIVAPLAIVCVVAFTPLGYISLPTDAL
                     SLRWFAAILDNPRFISAFWLSLGLGLVSSVIATALALPAALAIARFRFPGRDAISAFL
                     LSPLMIPHIVLGVAFLRFFSAIGINGTFGGLVMGHVIVILPFALRLVLASATGMDPAV
                     ERAAVSLGAGRLTVFRRITLPLILPGIVSGWLLAFITSFDELTMSVFIASPSTTTLPV
                     RMFLHIQDTIDPLVAAVSALLIAGTGAVMWVLDRLVGLERLFVGRGRHG"
     gene            7999..9117
                     /locus_tag="AC16HB_06795"
     CDS             7999..9117
                     /locus_tag="AC16HB_06795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606534.1"
                     /translation="MTQDYDIAVLGGGLVGGALAYGLARAGRRAALLDEGDIAFRASR
                     GNFGLVWVQGKGLGLPSYGGWTQHSARMWPQFARLLADETGIDVALGQPGGFHVCLSD
                     AEMAHRVAALTKLVEQPGFERYAFDVLDRAGVAARVPGIGPTVVGATYCALDGHCNPL
                     RLLRALHAAFEKRGGHYLPLHRAERIERNGDRFAIHTVAAQVRAQQVVLAAGLGNAPL
                     APQVGLHAPVRPQRGQVIALERVRPFLSYPMSTLRQTDEGTVLIGDSQEETGFDDSLA
                     TAVSATEAERAVRIFPGLRDVRVTRTWSALRVMSPDGFPIYEQSSSQPGAFLVTCHSG
                     VTLAAAHVLELAPRIAAGDLGSDLKPFSAERLNVSKAA"
     gene            9098..9409
                     /locus_tag="AC16HB_06800"
     CDS             9098..9409
                     /locus_tag="AC16HB_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5606535.1"
                     /translation="MFRRLPEAQAANVRVTIDGQPFAARDGDSVAATLFAAGVSACRT
                     TPVSGAARGPYCMMGVCFECLVTIDGRPNQQGCLVPVRAGMRIERQSGARISVDAQGR
                     P"
     gene            9406..>10216
                     /locus_tag="AC16HB_06805"
     CDS             9406..>10216
                     /locus_tag="AC16HB_06805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606536.1"
                     /translation="MNLATNYDVAVVGAGAAGLAAATECARAGLATVVFDEQSGPGGQ
                     IYRAITTTPLADRRVLGPDYWHGEGLASEFEHARADYVPRATVWAVTRSGGGFEIGVS
                     GDGTARLITAWQMILATGALERPFPIPGWTLPGVLTAGAAQILLKTSGLVPEGRVLLA
                     GTGPLLYLVAAQLARVGAKIDRLLDTTPRGRWREAASYALEFVTSPFFLKGLTLLREA
                     RAAARIVKGVERIEALGDDRLQRVRFVADGRSEELPADVLLLHQGVVPNINL"
BASE COUNT         1430 a         3510 c         3633 g         1643 t
ORIGIN      
        1 cgcagtcatg cgcgcaggcg acctgcagca cgcggattgc gccgctcgcg cggtcgactt
       61 cgacttccat cgcgaccgcg acgaaggtct cgttgtgctt gtagtggatg taagcgatgc
      121 cgcgcccgcg cgccttgccg cccgactgcc cggcgggcga cggccgcggc gtccagccga
      181 tcagcgccgc gcagcgctgc agcacctcga ccccgcgcgg gttggcgagg tcgcgcaggc
      241 ggaattcgac cggatccagc ttcgcgctcg cggcgagctg atcgatgaag cactcgacgg
      301 cgaagcagtt ggccaccttg cccggcgcac ggatgttcga cgggcgcagc ggcgcgtcgg
      361 caagccagtg tgcggtgacc ttgacgttcg cgaccgcgta cggcggatcg gcgttctgcg
      421 agatgagccc gaccgactgt cctgccggct gcgcgatgcc ggcgtcggtc ggcccgagca
      481 gcggcacatg cgacaggttg gcggtcgcca tcggcaccca cagctccgct tcccacgcgg
      541 caatgcggtt gtcgtcattc agcagcgccc gcagttcgat cagctgcggc gggcctttcg
      601 ggtcccaccc cagttcgtcc tcccgcatcc actgcacgcg caccgggcgg ccgaccgcct
      661 tcgacagcag caccgcatcg gcggccgcgt cgtcgtggcc gttcatgccg tagcagccgg
      721 cgccgtcgag gtagatcact ctcaccttgt cgcgcggcag ccccgctacg tgcgccagcg
      781 ccggctgata gcggtgcgtg gcctgcgacg cggaccagat cgtcgcggag tcggcccgca
      841 cgtccgccac cgcgcacgac ggccccatcg acgcgtgcga ctgcacgggc cagaagaagg
      901 tggcgcgcag cgcgcgcgtg tcagcgccga gcgccgccgt gctgcccttg ctgagcagca
      961 cgtcgtcctt cacgaatggg ccccgcttgg cccagtccct gacgccggca tcgccgacca
     1021 gcccgcggcc tgcgctccac ttcgccttca gttcgcgcgc cgcgcgcacc gcggtccatt
     1081 cgtcgttcgc caccacgccg aggaagttct cgatccgtac cacgcgtgcg ccgcgcaggt
     1141 gtccgatcga gctctcgtcg acttcgagca gcttcgcgcc gaccgtcgcc ggccgcagca
     1201 cgcgcccgta cagcatgttc gccagccgga agtcgtgcac gaacacgtgc cgccccgtca
     1261 actttgcagg caggtcgggg cgcggcagcg agcggccgac gagggagtag cgcagcggat
     1321 ccttcagcgc tgccttgggg ttcagcttca cgttgaacgg gcgggcgccg gcgagttcgg
     1381 cgatcgtgac cttcggcccg ccgtcggccg cgccgacgag gcctgcgtcg agcgtcggct
     1441 caccggcggg acgcttcagc cgggacgacg ccagtgcgag cagcgcctcg cgtgccgtcg
     1501 ccgcggcctg gcgcaactgc acgccgccgc gcatgacgcc agtcgagccc gccgtggggc
     1561 cctggtccgg cgtcagccac gtatcgcctt cgacgagttc gatgcgcgcg agatcgtcgg
     1621 cgtccatgtt cagttcctcg gccaccatct gccgcatcgc gatccggtgc ccggtgcaga
     1681 gatcgacctt gcctgtatag atgacgaccg tgccgtcctt gcgcacggcg agaaacgcgt
     1741 cgacttcggt cgggtcgagc gtatgcgtgc ccgcgccggc gctcggctgc gcgcgcacga
     1801 ccggcgcgcc ggcgaacgag aagccgacga ccagcgcggc cgtttgcagg aattcgcgcc
     1861 gcgccgatga ttcgatggtc atctcacgtc ctccccgcgt cggccgcgcg cagcacggcg
     1921 cgcagcacgc gatcgtgcga accacagcgg cacaggttgc cgtcgagcgc ggcctttgcc
     1981 tgctcccgtg tcggacgcgg gttcgatcgc agcagcgcga cggtcgacat cacgacaccg
     2041 gtcgtgcagt agccgcactg cgcggcctgc ccggcgatga aggcggcctg caccgggtgc
     2101 ggtttgtccg gcgtgccgag accctcgatc gtggtgatgc gttttccggc cacggcctgc
     2161 accggcgtca cgcaggcgcg cgcgggtcgg tcgtcgatca gcacggtgca actgccgcac
     2221 tggccgaccc cgcagccaaa ctttgcgccg tgcaggccga ggtcgttgcg cagcacgtac
     2281 agcagcggct gatcggcatc ccaggactcg acgcgctggc gcagattgtt gacgatcagt
     2341 tcgaatgcag gcatcggtgc ggcctcctcg tgcgcggcgt tctacgcgcg gcgcggcggg
     2401 aattcaatcg cggggacagc actggatcat ggcagcgccg gccgctttgt ccgatactcg
     2461 cgcgcgatgg cggctcgact cttccagggc gtcaagggtg tggccgcgac gctcgtgctg
     2521 gtgctcaaca cgctggtgtt cgtcaccgcg atggtgccgt tcgcactggt caagctgatc
     2581 gcccccgccg caccggtgcg gcgtgtggtc gatcgcgtgc tcaacgcgct tgcgagctac
     2641 tggatccgcg tcaacggcgc gtggatcgcg gcggtcgggc atccgcgctg ggacttgcag
     2701 ggcgtcgaag gcctgaagcg gcgtggttgg tacctgattg cgagcaacca cacgagctgg
     2761 gtcgacatcc tggtgctgca gaaggtgttc agcggccgca tcccgatgct gaagttcttc
     2821 ctgaagcagg agctgatctg ggtgccggtg atcgggctgg cctggtgggc gctcgacttc
     2881 ccgttcctga agcgcggtcg cggcggcgcg tcgggccagg tcgacctcga aagcacgcgt
     2941 cgcgcgtgcg agcgcttcaa gctgatcccg acctcggtga tcatcttcgt cgagggcacg
     3001 cgcttcacgc acgccaagca cgacgcgcag cgctcgccgt acgcgcacct gctgaaaccc
     3061 aagggcggcg gcctggcgat cgcgctggcg acgatgggcg gccaattcga cgcgctgctc
     3121 gacgtcacgc tcgcgtatcc cgacggcacg ccgacgttct gggacctgct gtcggggcgc
     3181 gtgcacgagg tcgcggtgcg cgtgcggcag cggccgatcc cgcccgcgct gcatgacgcc
     3241 agcgccaagc gaacgcaggt gcaggagtgg atcgacgcga tctggcgcga caaggatcgc
     3301 ttgctggacg aactgctcag ccgagcttca gcgacatcga gtagttcatc cggtggtgcg
     3361 gaaagatcgt gctcgtgacg agcacgctct tgccgctcgc gctgatgtaa cgccgaccga
     3421 tcacgaaccc cgcgtaaccc gcttcgacgc ccagcgcttc cgcctcgtgc ccgaccagcg
     3481 gcttggcgga gaactcctgc tcgacggtga cgatcggctc gccgatcttg tcctcgatga
     3541 agtcgtgcag ccccacgccg cggcggtcga gcacgccgcg cagcgcgggg tggtcgcggt
     3601 gcacgtacgc gagcacgtag gcgaccgggt ggcgcgcgcc gcgcaggtgc ttgagcgccg
     3661 tcagccgatg ccaggcctga ccgcgccggc acttcagcaa ccgcgcctcg gcggggctcg
     3721 ccttgatgtc gcgcgcctcc agcacctcga gctgcgcggt cttcacgtac gcgagcaggt
     3781 cctgcagcgc ggagatgcgc tgcgtgaagc cgcccgagcg tgtgcgccgc aggaccacgg
     3841 tgccggcgcc ggggcggcgc gcaatcaggc cctggtcggt caggcgccgg atcgcctcgc
     3901 gtaccgtgtg gcggctggcg cgaaacgtct tgcacagctc cagttcggtc ggcaggcggc
     3961 cgccgaccgg gtaacggccc gagcggatcc cgtcggcgag cacgcggaag atcgccgcgt
     4021 agcgcgtgtt gcgcgcgggc ggctcgcggg cgacggcggc ggcaaccatg gtgcggcggc
     4081 gatagcggcg atgcgtgcag cggcgcggct tgacaggcag tcgcggccgc gcgcagactt
     4141 tgtccggaca aactcatggt cgcatgcgcg cgcatcgttg gcaagcggcc gtcccgatcg
     4201 atcgccacag gaggagccat gaaacttcgc tgccgcgcgt cgtgcgtcgc gctcgcactc
     4261 accgccgcct tcgcatccgc cgccgcgctg gcgcagggca agctcaccgt cgccgcgtac
     4321 ggcggctcgt tcgaggacat gatgcgcaag gagatcatgc cggcgttcga gaagaagacc
     4381 ggcgccaagc tcgagtacgt ggccggcaac tcgaccgaca acatcgcgcg catgcaggcg
     4441 cagcgcgcca acatggaggt cgacgtcgcg atcgtcgacg acgggccgat gtaccaggcg
     4501 gtcgcgctcg ggttgtgcgg cgaactgaag ggcgtgaact tcggcgagct gtatccggtc
     4561 gcggacatga agaccggcaa gtcggtgacg atcggcgtgg tcgccaccgg gctgatgtac
     4621 aacaagaagt acttcgacga caacaagatg gcggcgccga cgtcgtggaa cgacatcaag
     4681 gatcagaagt accgcaagaa gctcgtgatc ccgccgctga acaacaccta cggcctgcag
     4741 accgtgatca tgatggcccg cctggccggc ggcggcgaga agaacatcga tccgggcttc
     4801 aaggtgatga aggacgaggt cgcgcccaac gtgctcgcgt tcgagccgca gccgggcaag
     4861 atgacggagc tgttccagag cgggcaggcg atcatcgccg tgtggggcag cggccgcgtg
     4921 aaggcgttcc aggacaccgg attcccggcc gagttcgtct atccgaagga aggcgcgatg
     4981 gcactcggca tctcggcctg cccgaccgcg aagtcgaatc cgtcgccgct cgcgatgcag
     5041 ttcatccagt tcctgctgca gcccgacacg caggcggtgt tcgccaaggg cgcgggagcg
     5101 ggcccggtga accgcaagac ggtgctgccg cccgagttgc agaagggcct gccgtatgga
     5161 gacgcggtcg gcaaactgtt gaccaccgac tgggacacgg tcaacgccaa ccgcaccgcg
     5221 tggaacaacc gcttcacgcg cgagatcgag cgctgagcaa cgcctgatgc gcagcgggcg
     5281 gtgagcttcc tcgtcctcga agggctgacc aagcgctacg gcgatcacgc cgcggtcgat
     5341 ggcatctcgc tcgcggtcgc gaagggcgag ttcgtgtcgc tgctcggccc gtcgggttgc
     5401 ggcaagacga cgacgctgca gatgatcgcc ggcttcgtcg agccgaccgc gggccgcatc
     5461 gccctcgagg ggcgcgatct gctcgtggtg ccggccgcga agcgcgggct gggcatcgtg
     5521 ttccagaact acgcgctgtt cccgcatatg acggtggtgg aaaacgtcgc cttcgggctg
     5581 gagatgcgca acgtcggcag gaccgaacgg cagcagcgcg tgaacgagac gctggcgctg
     5641 gtcggactgt ccggcttcgc cgagcgtcac ccggcgcagc tgtccggcgg ccaacagcag
     5701 cgcgtggcgc tggcgcgcgc gctggtgatc cgccccgaac tgctgctgct cgacgagccg
     5761 ctgtcgaacc tcgacgccaa gctgcgcgag gagatgcagg tcgagctgcg ccgcatccag
     5821 cgcgcggtcg gcatcacgac gctgatggtc acgcacgacc aggccgaggc actcgcgctg
     5881 tccgaccgtg tggtggtgat gcaccagggc cgcatcgagc agcaggacag cccgctcgcc
     5941 gcgtacgagc atccgcgcac gggcttcgtg tgcgacttcc tcggcaaggc caacctgttc
     6001 accgtcgagc gcgtcgacgc ggacgccatg aagatcggcg tgctcacgct gccgctgccg
     6061 gccggcgatc cggtgcgcgc cggcgatcgc atcctggtgc ggccggagaa gatctccttc
     6121 ggtgcgcccg gcccgggcgc gctggccgcg cgcgtgacca accgcgtgtt ccagggcaac
     6181 cactggctgt accagctggc caccccgctc ggcgatgcgc aggtgatccg tcagaacgac
     6241 ggcggcgcgt gcccggtcga aggcgacgag gtgcacctgg tgtggcgcgc cgacgcgatg
     6301 cgcgcggtcg ccgcgcactg aggcgcggga tggccgccgg caagcgcatc gccgcgtact
     6361 ggctgtcggc gccggcgctc gcggtctacg cgatcttcct cgtggtgccg atcgcgtcgg
     6421 tcgcgctgat cagtttccag agcttcgagt tctacggtgg catccagccc ggcttcacgc
     6481 tgaagaacta ccgcgaggtc ttcaccgaca gctactactg ggaggtgttc ggccgcacgt
     6541 tctggatcgc cgcggtggtc accgcgctgt ctgccgtgct cggcacgttc gaggcgatcg
     6601 tgctgcacaa gatggccaat ccgtggaaga gcttcttcct gctgatcgtg ctcggcccgc
     6661 tgctgatctc ggtggtggtg cgcacgctcg gctgggcgct gctgttcggc tccacgggtc
     6721 tcgtcaacca ggcgctgctc gcgctcggcc tgatccagga accgatcgct ttcatgtact
     6781 cggtgacggg cgtcgtgatc gcgctcactc acgtgctggt gccgttcgtg gtgatctcgg
     6841 tgtgggcgtc gctgcagcgg atcgatcctg ccgtcgagcg cgccgcagtg tcgctcggcg
     6901 cagggcagcc gaccgtggtg cggcgcgtga tcgtgccgct ggcgatgccg ggcatcctgt
     6961 cgggcagcgt gatcgtgttc gcgctggcag cgagcgcctt cgccaccccg gccatcatcg
     7021 gcgggcgccg cctgaaggtg atcgcgactg ccgcctacga cgagttcctg aacacgctga
     7081 actggccgct cggcgcctcg ctcgcggtgc tgctgctcgc ggccaatgta ctggtgctgt
     7141 acgcgtcgaa ccggatgatc gagcgccgct acaaggccgt gttccgggag agcgccgcat
     7201 gatccgcaat ggtgcgctcg cgctcgcgtt ccatgcgctg ttcatcggct tcatcgtcgc
     7261 gccgctggcg atcgtctgcg tggtcgcgtt cacgccgctc ggctacatct cgctgccgac
     7321 cgacgcgctg tcgctgcgct ggttcgcggc gatcctcgac aacccgcgct tcatcagcgc
     7381 gttctggctg agccttgggc tcgggctggt gtcgtcggtc atcgcgaccg cgctcgcgct
     7441 gccggcggcg ctggcgatcg cgcggttccg ctttcccggg cgcgacgcga tctcggcttt
     7501 cctgctgtcg ccgctgatga tcccgcacat cgttctcggc gtcgccttcc tgcgcttctt
     7561 cagcgcgatc ggcatcaacg gcaccttcgg cggactggtg atggggcacg tgatcgtgat
     7621 cctgccgttc gcgctgcggc tggtgctggc gtcggccacc ggcatggacc cggcggtcga
     7681 gcgcgccgcg gtgtcgctcg gcgccggccg cttgaccgtg ttccggcgca tcacgctgcc
     7741 gctgatcctg ccgggcatcg tcagcggctg gctgctcgcg ttcatcacca gcttcgacga
     7801 gttgacgatg agcgtgttca tcgccagccc gagcacgacg acgctgccgg tgcggatgtt
     7861 cctgcacatc caggacacga tcgatccgct ggtggccgcg gtgtcggcgc tgctgatcgc
     7921 gggcacgggc gcggtgatgt gggtgctcga tcggctggtc gggctggagc ggctgttcgt
     7981 gggccgggga cgacacggat gacgcaggac tacgacatcg ctgtgctcgg cggcggtctg
     8041 gtcggcggcg cgctcgcgta cggcctggca cgcgccggcc gccgcgcggc gctgctcgac
     8101 gagggcgaca tcgcgtttcg cgcctcgcgc ggcaacttcg gtctcgtgtg ggtgcagggc
     8161 aaggggctag ggttgccgtc gtacgggggg tggactcagc attcggcgcg catgtggccg
     8221 cagttcgcgc ggctgctcgc ggacgagacc ggcatcgacg tcgcgctcgg gcagccgggt
     8281 ggcttccacg tgtgcctgtc ggacgccgaa atggcgcacc gggtcgcagc gctgacgaag
     8341 ctcgtcgaac agccggggtt cgagcgctac gcgttcgacg tgctcgaccg cgccggggtc
     8401 gccgcgcgcg tgccgggcat cggcccgacc gtcgtcggcg cgacctactg tgcgctggac
     8461 ggacactgca acccgttgcg cctgctgcgc gcgctgcacg ccgcattcga gaaacgcggc
     8521 ggccattacc tgccgctgca ccgcgccgag cggatcgagc gcaacggcga tcggttcgcc
     8581 atccataccg tcgcagcgca ggtccgcgcg caacaggtcg tgctggccgc cgggctcggc
     8641 aatgcgccgc tcgcgccgca ggtcggattg cacgcgccgg tgcggccgca gcgcggacag
     8701 gtgatcgcgc tcgagcgggt gcgcccgttc ctgtcctatc cgatgtcaac gctgcgccag
     8761 actgacgagg gcaccgtgct gatcggcgat tcgcaggagg aaacgggctt cgacgattcg
     8821 ctcgctaccg cggtcagcgc cacggaggcc gagcgcgcgg tccgcatctt ccccggcctg
     8881 cgcgacgtgc gcgtcacgcg cacctggtcc gcgctgcgcg tgatgagtcc ggacggcttc
     8941 ccgatctacg agcaatcgtc gagccagccc ggcgcgtttc tcgtcacctg ccattccggc
     9001 gttacgctcg cggccgcgca cgtgctggag ctggcgccgc ggatcgcggc gggcgatctc
     9061 ggttccgatc tgaaaccgtt ctcggcggag agactgaatg tttcgaaggc tgcctgaggc
     9121 gcaagcggcg aacgtgcggg tcacgatcga cggccagccg tttgccgcgc gcgacggcga
     9181 ctcggtggct gccacactgt tcgcggccgg cgtgagcgcg tgccgcacca cgccggtgtc
     9241 cggcgcggcg cgcgggccgt actgcatgat gggcgtgtgc ttcgagtgcc tggtcacgat
     9301 cgacggccgg ccgaaccagc agggatgtct cgtgcctgtg cgcgccggca tgcgcatcga
     9361 acgccagtcc ggggcacgca tctcggtcga cgcacagggc cggccatgaa ccttgcgacc
     9421 aactacgacg tcgcggtcgt cggcgccggc gcggcggggc tcgccgcggc gaccgagtgc
     9481 gcgcgcgcgg ggctggcgac ggtcgtgttc gacgagcagt ccgggccggg tggtcagatc
     9541 tatcgcgcga tcacgacgac gccgctcgcg gatcgccgcg tgctcggtcc cgactactgg
     9601 cacggcgagg gcctcgccag cgagttcgag catgcgcgcg ccgactacgt gccacgcgcg
     9661 accgtgtggg cggtcacgcg ctcgggcggc ggtttcgaga tcggcgtgtc gggcgacggc
     9721 accgcgcgcc tgattaccgc gtggcagatg atcctcgcca ccggcgcgct cgagcggccc
     9781 tttccgatcc ccggctggac gctgccgggc gtgctgaccg ctggcgccgc acagatactg
     9841 ctgaagacgt cgggcctggt tccggaaggc cgggtcctgc tggccggcac cgggccgctg
     9901 ctgtacctgg ttgccgcgca actggcgcgc gtcggcgcga agatcgaccg cctgctcgac
     9961 accacgccgc gcggccgctg gcgcgaggcg gcgtcgtatg cactcgagtt cgtgacttca
    10021 ccgttctttc tcaagggcct gacgctgctg cgcgaggcgc gtgcggccgc gcggatcgtc
    10081 aagggcgtgg aacgcatcga ggcgttgggc gacgatcgac tgcagcgcgt gcgcttcgtc
    10141 gccgacggcc gcagtgaaga gttgcccgcc gatgtgctgc tgctgcacca gggcgtggtg
    10201 ccgaacatca acctgt
//
LOCUS       DBNHBY010000275         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1302587, whole genome shotgun sequence.
ACCESSION   DBNHBY010000275 DBNHBY010000000
VERSION     DBNHBY010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1302587"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="AC16HB_06810"
     CDS             complement(<1..1873)
                     /locus_tag="AC16HB_06810"
                     /inference="COORDINATES: protein motif:HMM:NF045284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR5606537.1"
                     /translation="MEVTDPAKIDRIEEVVKLALAHAPSGERAQIEMFAREYFRQVDP
                     DDIVERPAEELMGALLSHWQFGAKREPGKPKVRVLSPTAAEHGWVSRHTVIEIVNDDM
                     PFLVDSATMEINRRGSTLHQIVHPIFAVERGADGALKWIRPRGPHGTADDTPRESWMH
                     IEIDRLVEPQMRADVQEDIERVLGDVRAAVEDWKSMLARLREAIAELDRHPPTPPADE
                     VAEARAFLQWLFEDHIVLLGYRQHDLVTQDGDDALKLVAGSGLGLLRQKPGEKVSESF
                     ASLPAAARALARKPSPVVIVTKANTRATVHRPGYTDYIGVKRFNDKGDVIGEHRFIGL
                     LTSTAYSARVTEIPLLRGKLARVAERAGFLTGSHLAKALDHVFETFPRDELFQISTED
                     FYEIALGILQLGERQRLRIFIRPDQFERFVSCLVFVPRDVYSTDLRLKFQQILIDAFD
                     GIGAEFDVLLSDAILARIQFTVRTTPGRLPVYDRHQIERKLAAAARRWDDELREALIE
                     DEGEARGIQMYKRWAGTFPAAFSERVTPRAAVADVRKIDGLTKESPFALSFYRPLGSN
                     DTMGFKIYHAGAPVVLSESLPMLEHMGVRLLSENPYRVGPDGAAQVWIHDFSLQAQVA
                     "
     gene            1943..>2563
                     /gene="aroC"
                     /locus_tag="AC16HB_06815"
     CDS             1943..>2563
                     /gene="aroC"
                     /locus_tag="AC16HB_06815"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5606538.1"
                     /translation="MSGSTIGHLFRVTNFGESHGPAIGCVIDGCPPGLELSADDLQPD
                     LDRRKPGTSRHVTQRRESDRVEILSGVYLGRTTGAPIALLIRNEDPKSRDYEDLVDTF
                     RPGHADYTYWRKYGFRDPRGGGRSSARLTAPMVAAGAVARKWLREKFGTRVRGCMQQL
                     GSIAVPFVSWEHVDANPFFAPNAAIVPRLEEYMDALRKRGDSCGARI"
BASE COUNT          398 a          851 c          880 g          434 t
ORIGIN      
        1 ccgcgacctg cgcctgcagg ctgaagtcgt gaatccacac ctgcgcggcg ccgtcggggc
       61 cgacccggta cgggttttcg gagagcaagc gcacgcccat gtgctccagc atcggtaggc
      121 tctccgacag cacgaccggc gcgccggcgt gatagatctt gaagcccatc gtgtcgttgg
      181 agccgagcgg acggtagaaa ctcagcgcga acggtgattc cttcgtcagt ccgtcgatct
      241 tgcgcacgtc ggccaccgcc gcgcgcgggg tcacgcgctc gctgaacgcg gcgggaaagg
      301 tgccggccca gcgcttgtac atctggatgc cgcgcgcttc gccctcgtcc tcgatcagcg
      361 cttcgcgcag ttcgtcgtcc cagcggcgtg cggcggcggc gagcttgcgt tcgatctggt
      421 ggcggtcgta cacgggaagt cttcccggtg tcgtacggac ggtgaactgg atgcgcgcca
      481 ggatcgcatc ggacagcagc acgtcgaact cggcgccgat gccgtcgaat gcgtcgatca
      541 ggatctgctg gaacttgagc ctcagatcgg tcgaatacac atcgcgcggc acgaacacca
      601 ggcacgagac gaagcgctcg aactggtcgg gccggatgaa tatgcgcagc cgctggcgct
      661 cgcccaactg caggatgccg agcgcgatct cgtagaagtc ctcggtcgag atctggaaca
      721 gctcgtcgcg cgggaaggtc tcgaacacgt ggtcgagcgc cttggccaga tgactgccgg
      781 tcaggaaacc tgcacgctcc gcgaccctgg ccagcttgcc ccgcaacagc ggaatctccg
      841 tcacgcgggc gctgtaggcg gtcgaggtga gcaggccgat gaaacggtgc tcgccgatca
      901 cgtcgccctt gtcgttgaag cgcttgacgc cgatgtagtc cgtgtagccg gggcggtgca
      961 cggtggcgcg cgtgttggcc ttggtgacga tgacgaccgg cgacggcttg cgcgccagcg
     1021 cgcgcgcggc cgcaggcagc gacgcaaagc tttcggacac cttctcgccg ggcttctggc
     1081 gcagcaggcc gagtccgctt ccggcaacga gcttcagcgc atcgtcgccg tcctgcgtga
     1141 cgaggtcgtg ctggcggtag ccgagcagca cgatgtggtc ctcgaacagc cactgcagga
     1201 acgcgcgcgc ctcggcgacc tcgtctgcag gcggcgtcgg cggatggcgg tcgagttcgg
     1261 cgatcgcctc gcgcaggcgc gcgagcatcg acttccagtc ctcgaccgcg gcgcgcacgt
     1321 cgccgagcac gcgctcgatg tcctcctgca cgtcggcacg catctgcggc tcgaccaggc
     1381 ggtcgatctc gatgtgcatc cacgattccc gcggagtgtc gtcggcggtg ccgtgcgggc
     1441 cgcgcggacg gatccatttc agcgcgccgt cggcgccgcg ctcgaccgcg aagatcgggt
     1501 gcacgatctg gtgcaatgtc gagccgcggc ggttgatctc catcgtggcc gaatcgacca
     1561 ggaacggcat atcgtcgttg acgatctcga tcaccgtgtg gcgcgacacc caaccgtgct
     1621 cggccgcggt cggtgagagc acgcgcacct tcggcttgcc cggctcgcgc ttcgcgccga
     1681 actgccagtg cgacaagagc gcgcccatta gttcctcggc ggggcgttcg acgatgtcgt
     1741 cggggtcgac ctggcgaaag tactcgcgcg cgaacatctc gatctgcgcg cgctcgcccg
     1801 agggcgcgtg cgctagcgcg agcttcacga cctcctcgat gcgatcgatc ttggccggat
     1861 cggtgacttc catgtcggtc ccccgtcttc ttcttgggtc ggcgtgcgca tttatacgcg
     1921 caaggccact tacaatgccc gcatgtcagg ctcgaccatc ggccaccttt tccgcgtcac
     1981 gaacttcggc gaatcgcacg gtccggcgat cggctgcgtg atcgacggct gtccgccggg
     2041 actcgaactg tcggccgacg acctgcagcc cgacctcgat cggcgcaagc ccggcacatc
     2101 gcgccacgtg acgcagcggc gcgaatcgga tcgcgtcgag atcctgtccg gtgtgtacct
     2161 gggcaggacg acgggcgcgc cgatcgcgct gctgatccgc aacgaggacc cgaagagcag
     2221 ggactacgag gatctcgtcg acacgttccg gcccggtcac gccgactaca catactggcg
     2281 caagtacggc tttcgcgatc cgcgcggcgg cgggcgctcg tcggcgcggc tgaccgcgcc
     2341 gatggtcgcg gccggcgccg tggcaaggaa gtggctgcgc gagaagttcg gcacacgcgt
     2401 gcgcggctgc atgcaacagc tcgggtcgat cgcggtgccg ttcgtttcgt gggagcatgt
     2461 cgatgcgaat ccgttcttcg cgccgaacgc ggcgatcgtg ccgcggctcg aggagtacat
     2521 ggatgcgctg cgcaagcgcg gcgattcgtg cggcgcgcgc atc
//
LOCUS       DBNHBY010000276         3482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1302625, whole genome shotgun sequence.
ACCESSION   DBNHBY010000276 DBNHBY010000000
VERSION     DBNHBY010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1302625"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..626)
                     /locus_tag="AC16HB_06820"
     CDS             complement(63..626)
                     /locus_tag="AC16HB_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606539.1"
                     /translation="MNVERLLAPDDQLAATADDLARAPGASLGRLIVRMLAGAAAAGL
                     AIYIGTHWLTTFWDFELGFVPVFEGRVDPLRAMVFSMLAGPLLLASAFTLVAPFFGAP
                     RRPLAALAVAVIGMTPIYAAGALMFFLPAVLLMLLAFIVSCFRWGRGAQRLLGVPERE
                     TAEFIAITLLATSLALQIASHLAADLL"
     gene            complement(623..1126)
                     /locus_tag="AC16HB_06825"
     CDS             complement(623..1126)
                     /locus_tag="AC16HB_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5606540.1"
                     /translation="MNLKRWLKHWLITPRRVHRVLPQESLERIRAAIAQTETTHAGEI
                     RFAVEASLPWSYLRRNAPARQRATMVFAKLRVWDTEANNGVLLYVDLADRAIEIVADR
                     AIARHVHRHEWEAICNDVREHFRRNDFEAGVIAGVQAIGALLARHFPLAPGQVNPNEL
                     SDKPAVL"
     gene            complement(1126..2034)
                     /locus_tag="AC16HB_06830"
     CDS             complement(1126..2034)
                     /locus_tag="AC16HB_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5606541.1"
                     /translation="MTRWLRALAALLLFTVAAGAFAQARTPDGLLAIPPLARVTDATG
                     ALSAADKQALEDKLAAFETAHGSQIAIVMVASTQPEPISDYAQRIGDAWKIGRAGIGD
                     GLLIVVAKDDRKVWIAVAKALEGAIPDVAAKRVIREAIAPRFRNNDFAGGLSAGLDSI
                     FKLIEGEGLPTPAGVSERQVDAGEGALALLVPFIMVGVVVGAILRRVLGRPGALLAGG
                     GAGAVAGWMLSSLALGAVAGFAVLIFSLAGGASSAGRVLGGRRGGVFLPGGWSGGGGW
                     SSGGGGGGWSSGGGGDFGGGGAGGDW"
     gene            complement(2051..2647)
                     /locus_tag="AC16HB_06835"
     CDS             complement(2051..2647)
                     /locus_tag="AC16HB_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5606542.1"
                     /translation="MRVRALIAVVLVSLLAGCGYNEIQSKDEAVKAAWSEVLSQYQRR
                     ADLIPNIVNTVKGEANFEQETLTRVIEARAKATSIQATPELVNDPAALAKFQQAQGEL
                     SSALSRLMVVVERYPDLKTNAGFQDLRTQLEGTENRITVARNRYIKSTQDYNVLVRQF
                     PVNLTAMVFGYKEKAQFAVENEAVISKPPTVDFGAPKK"
     gene            complement(2723..3112)
                     /locus_tag="AC16HB_06840"
     CDS             complement(2723..3112)
                     /locus_tag="AC16HB_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR5606543.1"
                     /translation="MLLNGLPGLAALVERRIEEAVARGEFDNLPGAGRPFDLDDDLLV
                     PVDMGVANRILKNAGYVPPDAQQMAEINRLIAQIEREEIAPAADAPKARRLRALLIQL
                     EMSGRHAMTRAAWVRYEEALAARFARA"
BASE COUNT          565 a         1253 c         1153 g          511 t
ORIGIN      
        1 gggctccgat acaaagaggg aggtgcagcc gtttggtcgg cgcgggggcg cccagcttac
       61 ggctagagga ggtcggccgc gaggtggctc gcgatctgca gcgcgagcga cgtggcgagc
      121 agcgtgatgg cgatgaactc cgccgtttcg cgctccggaa cgccgagcag ccgctgtgcg
      181 ccgcggcccc agcggaagca gctgacgatg aaggcgagca gcatcagcag cacggccggc
      241 agaaagaaca tcagcgcgcc cgcggcgtag atcggagtca tgccgatcac cgcgaccgcc
      301 agcgccgcaa gcggccgccg cggcgcgccg aagaacggcg ccaccagcgt gaacgcggat
      361 gcgagcagga gcggaccggc gagcatcgag aacaccatcg cgcgcagcgg atcgacgcgt
      421 ccctcgaaca ccgggacgaa cccgagctcg aagtcccaga acgtggtcag ccagtgcgtg
      481 ccgatgtaga tcgccagccc ggccgctgcc gcgccggcca gcattctcac gatcagccgc
      541 ccgaggctcg cgcccggcgc gcgcgcgaga tcgtccgcgg tcgccgccag ttggtcgtcg
      601 ggagcgagca ggcgttcgac gttcacagca ccgccggctt gtcggaaagc tcgttggggt
      661 tgacctggcc aggtgcgagc ggaaagtgcc gcgccagcaa cgcaccgatc gcctgcacgc
      721 ccgcgatcac gccggcttcg aaatcgttgc gacgaaagtg ctcgcgcacg tcattgcaga
      781 tcgcttccca ttcgtgacga tgcacgtggc gcgcaatggc gcggtcggcg acgatctcga
      841 tcgcgcggtc ggccagatcc acgtacagca gcaccccgtt gttcgcctcc gtgtcccaca
      901 cgcgcagctt ggcgaacacc atggtcgcgc gctggcgcgc gggcgcgttg cgcctcagat
      961 agctccacgg cagagacgcc tcgaccgcaa agcggatttc gccagcatgc gtggtttcgg
     1021 tctgcgcgat cgcggcgcgg atgcgctcga gcgattcctg tggcagcacc cggtgcacgc
     1081 ggcgcggcgt gatcagccaa tgcttcaacc agcgtttcag attcatcacc agtctccgcc
     1141 cgcgccgccg ccgccgaagt cgccgccgcc gcccgaactc cagccgccgc ccccgccgcc
     1201 ggacgaccag ccgccgcctc cgctccatcc gcccggcaag aacacgccgc cgcgcctgcc
     1261 gccgagcacg cgcccggcgc tcgacgcgcc gccggccagc gaaaagatca gcaccgcgaa
     1321 gcccgccacc gcgccgagcg ccagcgacga cagcatccac ccggccactg cgcccgcgcc
     1381 gccgccggcg agcagcgcgc cgggccggcc aaggacacga cgcaggatcg cgccgacgac
     1441 gacaccgacc atgatgaacg gcaccagcag cgcgagcgcg ccctcgccgg catcgacctg
     1501 gcgttcggaa acgccggccg gcgtgggcag cccttcaccc tcgatcagct tgaatatcga
     1561 atcgagcccg gccgacaggc cgccggcgaa gtcgttgttc ctgaagcgcg gcgcgatcgc
     1621 ctcgcggatc acgcgcttgg ccgcgacatc gggaattgcg ccttcgagcg ccttggccac
     1681 ggcgatccag accttgcggt cgtccttcgc cacgacgatc agcagcccgt cgccgatgcc
     1741 ggcacggccg atcttccatg catcgccgat gcgctgcgcg tagtcggaga tcggctccgg
     1801 ctgcgtgctc gcgaccatga cgatcgcgat ctgcgagcca tgcgccgtct cgaacgcggc
     1861 gagcttgtct tcgagcgcct gcttgtcggc cgccgacagc gcgccggtgg cgtcggtcac
     1921 gcgcgcgagc gggggaatcg cgagcagccc gtccggcgtg cgcgcctgag cgaacgcgcc
     1981 ggcggccacc gtgaacaaca gcagcgcggc gagcgcgcgc agccagcggg tcatgtcgag
     2041 cgatcaggcg ctacttcttg ggcgcaccga aatcgaccgt cggcggcttg ctgatgaccg
     2101 cttcgttctc gaccgcgaac tgcgccttct ccttgtagcc gaacaccatc gcggtgagat
     2161 tcaccgggaa ctggcgcacc agcacgttgt agtcctgcgt ggacttgatg tagcgattgc
     2221 gcgcgaccgt gatgcggttc tcggtccctt ccagttgagt ccgcaagtcc tgaaagccgg
     2281 cgttggtctt caggtccgga tagcgctcga ccaccaccat caggcgcgac agcgcgctcg
     2341 acagttcgcc ctgcgcctgc tggaacttcg cgagcgcagc cggatcgttg acgagttccg
     2401 gcgtggcctg gatcgaggtc gccttggcgc gtgcctcgat cacgcgcgtc agcgtctcct
     2461 gctcgaagtt cgcctcgccc ttgacggtgt tgacgatgtt ggggatcagg tcggcgcggc
     2521 gctggtactg cgacagcacc tcggaccacg cggccttcac cgcttcgtcc ttcgactgga
     2581 tctcgttgta gccgcagccg gccagcaacg acaccagcac cacagcgatc agcgcgcgca
     2641 cacgcatggt ttctcctcga cggttggtga acaggcgccg ctcagatggc gccacagcgg
     2701 attatttcaa gttccgcgcc aatcaagcgc gcgcgaagcg cgcggccagc gcctcctcgt
     2761 agcgcaccca ggcggcgcgc gtcatcgcat gacggcccga catctcgagc tggatcagca
     2821 gcgcgcgcaa ccggcgcgcc ttcggcgcgt cggcggcggg cgcgatctct tcgcgttcga
     2881 tctgcgcgat cagccggttg atctcggcca tctgctgcgc gtccggcggc acgtagcccg
     2941 cgttcttcag gatgcggttg gccacgccca tgtccaccgg cacgagcagg tcgtcgtcta
     3001 agtcgaacgg ccggccggca ccgggcaggt tgtcgaactc gccgcgcgcg accgcctcct
     3061 cgatgcgccg ttcgaccagc gccgcgaggc cggggagtcc gttcagcagc attcgcggat
     3121 ggtagccgca aacgtccccg ggtccgcacg gccccgaatg tcgttcggcc cgtccgcgcg
     3181 attgcggttc atcaacttgc ttcggagcag gcttcgcaca atgcatccaa gccgctgcgt
     3241 ttcgcagcgc ggaccgcgca cccgcccggt cgatgctgca gcagtcgatg cagcggcatg
     3301 aggagggcac tatgaacggc aaggtgattg cggtcctggt cgcgagcgcg ctcgccattc
     3361 cggcgctggc gcagcagacg tccgatctcg gcaagtacga atacttctcc aaatgcgcgc
     3421 cgtgccatgg cgccgccggc aagggcgacg gcccgatgcg caagcagctc gtcaccgcac
     3481 cg
//
LOCUS       DBNHBY010000277         2129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1235407, whole genome shotgun sequence.
ACCESSION   DBNHBY010000277 DBNHBY010000000
VERSION     DBNHBY010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1235407"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="AC16HB_06845"
     CDS             <1..684
                     /locus_tag="AC16HB_06845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5606544.1"
                     /translation="GDCVTSFEYLPRIAVELTVRHVPGVQDPFPGAYPAYVLCETASS
                     RDDPQLRERVEDTLASAIDAGLALDAAFAESLAQREAMWKLRESVPEAQRHEGASLKH
                     DVAVSVAALPEFLERASAAVRRVTPRGRLVAYGHVGDGNLHFNVSEPGPAGERDAFLA
                     AEEALHRAVHDCVQEFAGSISAEHGIGQLKRAALAHYKNPVALDVMRAIKRALDPHGI
                     MNPGKVLPD"
     gene            846..>2129
                     /locus_tag="AC16HB_06850"
     CDS             846..>2129
                     /locus_tag="AC16HB_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5606545.1"
                     /translation="MEFLRALLKSKLFRTAALVAGLVGLYALLGFYAAPRLVRSQAIE
                     YVRETYGRELAIGEVRIHPFKLQAEVRDVALPDTDGQPMLAFQRLFADFELSSLWHRA
                     FVFREVLLEAPFARAVIRRDGSVNLADLAPKEETPDEGPLPRVWIQHFGLSNGKVDFA
                     DQQRRRPLERAFAPVNFELEDFKTTPEGGDFGLVARSRADEEFEWHGKFALEPQVSSS
                     GNFAVSRLQLTNVAELLGDALPFVLSRGSMDLAGSYSFALPKDAPLQLTLALPQVKVT
                     DIGLQPYGVAEDWVTVPSLVVSDTKVALPAQTVALGQIALDGLRTRVWMNADGSLSTD
                     QLFAGAPAEGAVPAATEPVQAPAAEPPAAAGPVPASGTSPSAPAQAAAPAAAGEWTVN
                     VGAVALRQAAIDFEDRTVTPAATFALAPVNVSLRDA"
BASE COUNT          318 a          761 c          742 g          308 t
ORIGIN      
        1 ggcgactgcg tgacgagctt cgagtacctg ccgcgcatcg ccgtcgagct gaccgtgcgc
       61 cacgtgccgg gcgtgcagga tccgtttccg ggcgcctacc ccgcctatgt gctgtgcgag
      121 accgcgagca gccgcgacga cccacagttg cgcgagcggg tggaggacac gctggcgtcc
      181 gcgatcgacg ccggcctcgc gctcgatgcc gctttcgccg aatcgctggc acagcgcgag
      241 gcgatgtgga agctgcggga gtcggtgccc gaggcccagc gccacgaggg cgccagcctc
      301 aagcacgacg tcgccgtgtc ggtcgccgcc ctgccggagt tcctggaacg cgccagcgcg
      361 gccgtgcgac gcgtcacgcc gcgagggcga ctcgtcgcct acgggcacgt cggcgacggc
      421 aacctgcact tcaacgtcag cgagcccggt ccggccggcg agcgcgacgc cttcctcgcc
      481 gccgaggagg ctttgcaccg ggcggtgcac gactgcgtgc aggagttcgc cggcagcatc
      541 agcgccgagc acggcatcgg gcagctgaag cgggcggccc tggcgcacta caagaatcca
      601 gtcgcgctcg acgtgatgcg cgccatcaag cgggccctcg acccgcacgg catcatgaac
      661 ccgggcaagg tcctgccgga ctgagcgcat cccctggcgt tcgcgctgcg aaacgaatcg
      721 cgacggcccc ggttccggtc ggtcgcttgt gggccgtcac ccgagtgcct acagtccgct
      781 cgggggagga tgacgcagcg gcctggcccg gacggcgccg cgcttagaaa aagccacatg
      841 aagccatgga attcctgcgc gcgctgctga agtcgaagct gttccggacg gcggcccttg
      901 tcgcgggcct ggtcggcctg tatgcgctgc ttgggtttta cgcggcaccc cgtctggtgc
      961 gcagccaggc catcgaatac gtgcgcgaga cctacggacg tgaactggcg atcggcgagg
     1021 tccgcatcca cccgttcaag ctgcaggcgg aggtgcgcga cgttgcgctg ccagacacgg
     1081 acggccagcc gatgctggcc ttccagcgcc tgttcgccga cttcgagctg tcgtccctgt
     1141 ggcaccgcgc gttcgtgttc cgcgaggtgc tgctggaagc gccgttcgcg cgcgccgtga
     1201 tccgccgcga cggctcggtg aacctcgccg acctcgcacc gaaggaagag acccccgacg
     1261 aaggcccctt gcccagggtc tggatccagc acttcgggct cagcaacggc aaagtcgatt
     1321 tcgcggacca gcagcggcgc cgccccctcg agcgcgcctt tgcgccggtg aacttcgagc
     1381 tcgaggattt caagacgacc cccgaaggcg gcgacttcgg cctcgttgcg cgcagtcgtg
     1441 ccgacgagga gttcgagtgg cacggaaagt tcgcgctcga gccgcaggtc agctccagcg
     1501 gcaacttcgc ggtcagcagg ctgcagctga ccaatgtcgc ggagctgctc ggcgacgcgc
     1561 tgccgttcgt gctgtcgcgc ggcagcatgg acctcgcggg ctcgtacagt ttcgccctgc
     1621 cgaaggacgc gccgctgcag ctcacactcg cgctgccgca ggtcaaggtc accgacatcg
     1681 ggctgcagcc ttacggcgtc gccgaggact gggtcaccgt gccgtcgctg gtggtgagcg
     1741 acacgaaggt cgcgctgccg gcccagacag tcgcgctcgg gcagatcgcg ctcgacgggc
     1801 tgcgcacgcg cgtctggatg aacgccgacg gctcgctcag caccgaccag ctgttcgccg
     1861 gggccccagc cgagggcgcc gtacccgccg cgaccgaacc tgtacaggcg cccgctgcgg
     1921 agccccctgc tgcagcggga ccggtgccgg cgagcggaac cagcccgtcc gcgccggccc
     1981 aggccgcggc accggcagcc gcaggcgagt ggacggtcaa cgtcggtgcg gtcgcactgc
     2041 gccaggcggc gatcgatttc gaagaccgca ccgtgacgcc cgcggccacg tttgccctgg
     2101 cgcccgtcaa cgtctcgctc cgcgatgcc
//
LOCUS       DBNHBY010000278         1827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_557783, whole genome shotgun sequence.
ACCESSION   DBNHBY010000278 DBNHBY010000000
VERSION     DBNHBY010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_557783"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1446)
                     /locus_tag="AC16HB_06855"
     CDS             complement(19..1446)
                     /locus_tag="AC16HB_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIR5606546.1"
                     /translation="MSDTSPTVEAAAEFLENHPLAQIRVGDGARLVRTVTNRDIQLFA
                     AVSGDVNPAHVDAQYAKSSQFHEIIAHGMLGGALISTVLGTRYPGPGMTYLGQTLRFL
                     KPVHIGDTLAVSVTVTSADPATRRVILATRCIDQDGAVVIDGEAEVLAPAESLRIRRT
                     DLPEPLLADKNLRYDQLLAGAKQFPAVRVGVVHPCSREALDGALSAARLGLIVPVLIG
                     PQAKIQSVAAECGYALDGIALRDVPHSHAAAAAGVALARAGEVEALMKGSLHSDELMT
                     EVVNATSGLRTARRISHVFVLDVPRYPKPLLISDAAINIEPDLDAKADIVRNAIDLAH
                     ALNIAEPKVAILSAVETVSSKMRSSMDAAALAKMADRGQITGGIVDGPLAFDNAVSLA
                     AARTKGIRSPVAGDADVLIVPDIESGNMLAKQLEHLGDAQAAGIVLGARVPIVLTSRA
                     DSAHSRIASCAIALILAHRYRVVPP"
BASE COUNT          289 a          624 c          615 g          299 t
ORIGIN      
        1 ggatcagtgg gtcggcggtc atggcggcac gacccggtag cgatgcgcca ggatcagcgc
       61 gatcgcgcac gacgcgatgc gcgagtgcgc gctgtcggcg cggctggtca gcacgatcgg
      121 tacgcgcgcg ccgagcacga tgccggccgc ctgcgcgtcg ccaaggtgtt caagctgctt
      181 ggcgagcatg ttgcccgact cgatgtcggg aacgatcagc acgtcggcgt cgccggcgac
      241 gggcgagcgg atgcccttgg tcctggcggc ggccagcgac accgcattgt cgaatgccag
      301 cggcccgtcg acgatgccgc ccgtgatctg gccacggtcg gccatcttgg ccagtgcggc
      361 agcgtccatc gacgagcgca tcttcgacga gacggtttcc actgccgaca ggatcgcgac
      421 cttcggctcg gcgatgttca gcgcatgcgc caggtcgatc gcgttgcgca cgatgtccgc
      481 cttcgcgtcc agatcgggct cgatattgat cgctgcatcg ctgatcagca gcggcttcgg
      541 atagcgcggc acgtcgagca cgaacacatg gctgatgcgc cgcgcggtgc gcaggccgga
      601 agtcgcgttc accacctcgg tcatcagttc gtcgctgtgc aggctgccct tcatcagcgc
      661 ctcgacttcg cccgcgcgcg ccagcgccac gcccgcggcc gccgccgcat ggctgtgggg
      721 cacgtcgcgc agcgcgatcc cgtcgagtgc atatccgcat tcggccgcga cgctctggat
      781 cttcgcctgc gggccgatca gcaccggaac gatcaatccc agccgcgccg cggaaagtgc
      841 gccgtcgagc gcctcgcggc tgcagggatg gaccacgccc acgcgcacag ccggaaactg
      901 tttcgcgcct gcgagcaact ggtcgtagcg caggttcttg tcggccagca gcggctcggg
      961 caggtcggtg cgccggatgc gcaggctctc ggccggtgcc aggacctcgg cttcgccgtc
     1021 gatgacgaca gcgccgtcct ggtcgatgca acgcgtcgcc aggatcacgc gtcgtgtcgc
     1081 cggatcggcc gacgtgaccg tgaccgacac cgccagcgtg tcgccgatgt gcaccggctt
     1141 caggaagcgc agcgtctggc ccaggtaggt catgcccgga cccggatagc gcgtgccgag
     1201 cacggtcgag atcaacgcgc cacccagcat gccgtgggcg atgatctcgt ggaactggct
     1261 cgacttcgcg tactgcgcgt cgacatgcgc cggattcacg tcgccggaca ccgcggcaaa
     1321 gagctggatg tcgcggttcg tcaccgtgcg caccaggcgc gcgccgtcgc ccacgcggat
     1381 ctgcgcgagc gggtggttct cgaggaattc agcggccgct tcgaccgtgg gagatgtgtc
     1441 gctcatacgg atcctgctca agacggcgca aacctcgaag cccggtgtcc ggacgcgccg
     1501 tcgaggaacg gtgcagatcc gtgcacgcag cctggcgcgg cggcagggct cgccggattc
     1561 aacgacacat ccggggatcc atcgccgcca taccctcgtc gatcatcgta aggggccgat
     1621 ttcatacgac gctgtcgaat gtcaatctgc cgatgacccg gaggggactg cttcgcgatg
     1681 ggttccgaca ctacctttcc agcgcccgca gtccgcatcg gccgggagtc cggccgcaac
     1741 tcgtcgcggc aatcggcttc gaggtcctga caggccattc atcgcgcatc cgcgcggatc
     1801 gcattgttac gtacgccgca atgaaca
//
LOCUS       DBNHBY010000279         5327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1775697, whole genome shotgun sequence.
ACCESSION   DBNHBY010000279 DBNHBY010000000
VERSION     DBNHBY010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1775697"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC16HB_06860"
     CDS             <1..441
                     /locus_tag="AC16HB_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606547.1"
                     /translation="RFAGGRESIDERVKRYSNRAESWFGSVTAEQTQILHAALATATE
                     TWWLEERERRQHELMRLLRGLHDEKPGAAEATRRVRDYFAQLTEPHDEVRRASLLEYR
                     RSNAELLARLINAATPTQRATLAKRLRNYAEDFTALADASKGRG"
     gene            complement(449..1588)
                     /locus_tag="AC16HB_06865"
     CDS             complement(449..1588)
                     /locus_tag="AC16HB_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606548.1"
                     /translation="MKLRDLLRFAMVMTAVSLAACGKKEEAPPPKAEAKAEAKAAPAK
                     PTGTLNLYNWNDYIAAETIERFEKAFNAKVMQTYFSDNDEMLAKLAAGATGYDIIVPT
                     SNAMELMIKKGDLQPLDPAQLPNLKNIRPEFLNTPIDPGNKFSVPYAYSTTIIGYNDK
                     HMKQLGIEPRGWEAIFDPKIACRVKGKLTVLDSPDEVFSAAFIYLGIDPNTTSADDYK
                     KAAEAIRKAKPCWAAFNSSSYIKELTAGNIWLALGYSTDMFQANRNAQEAKQDFRIVS
                     VLPTQGAVLSVDNMVIHKAAPNAALAHAFINFMLEGQNSSELSNLIGSGNANQAAMEF
                     IKPEVKSNVAVFPDAESLKKLHQLRLLSAELRQLRGQLWTEIKIR"
     gene            complement(1613..2392)
                     /locus_tag="AC16HB_06870"
     CDS             complement(1613..2392)
                     /locus_tag="AC16HB_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606549.1"
                     /translation="MNRRVPAWMWACAAAAYVFLYAPLVVVVVFSFNDSKLNAQWVGF
                     TTAWYVKLFHNEEMMRAARNSLAIALVTSLASTVLGTMAGFVMHRYRTRVLPVLVLAP
                     IAIPEILMGVALLMFFVMINLTLGLVSITLAHVAFCIGYVAIVVRARLAGMDESLVEA
                     ARDCGASAPAAFRYVTLPLIMPGVIAGSLMAFTLSIDDFVITFFTAGASASTLPLQIY
                     SMVKVAVTPEVNAISTLLLALTLALITLATKFVPGLLRLNR"
     gene            complement(2389..3267)
                     /locus_tag="AC16HB_06875"
     CDS             complement(2389..3267)
                     /locus_tag="AC16HB_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606550.1"
                     /translation="MATAERGKWLVGGPPFVYLIVFFAIPSLLMVVASVRAPGEFGGL
                     APLVTDSGRLNLTWASYARFFASPIYLELFARSLAYALLTTALCLAIAYPLSMLIAAS
                     SKRYRDLLLLLVILPFWSNFLIRVYAWMIILGPEWALARLVNGVLGLFGAEPVTLMFS
                     PFAVIVVLVYVHLPFMVLPLYANLEKHDPALLDAAQDLGARPWQRFWHVTWPLSLPGV
                     FAGAALVFIPAFGIFAVPDLVGGTSGIMIGNVIKQQFLETRDWPFGSMLSIVLTLAAL
                     AVTALAALAARPRRSP"
     gene            complement(3269..4357)
                     /locus_tag="AC16HB_06880"
     CDS             complement(3269..4357)
                     /locus_tag="AC16HB_06880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606551.1"
                     /translation="MALLEIQNVTRRFGSMTAVDGVSLSIEAGEFFTLLGPSGCGKTT
                     LLRMIAGFDASDGGRIVLDGKDLTGVPPERRNVRTVFQSYALFPHMTVAANVAFPLKM
                     AKTPAGELGAKVREALADVQLDALAARFPHELSGGQRQRVAIARALVTHPALMLLDEP
                     LAALDAKLREQMQIELINLQKEVGITFVYVTHDQGEALALSHRIAVMNQGRIEQLDEP
                     SRIYSYPKNRFVADFIGQCNILEATVGRRDGTVAELDLPGVGPARALIDAALASGVRG
                     ALALRPEKVEINPSGQPKTDGDNHVSGTVTDFLYLGDVTLYRVALASGRKIEAMRANS
                     AAGRARFFEVGDAVDVAWQVGAGHFLFD"
     gene            complement(4380..5015)
                     /locus_tag="AC16HB_06885"
     CDS             complement(4380..5015)
                     /locus_tag="AC16HB_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5606552.1"
                     /translation="MTGFTTRDASRPEFWDERFAAGFTPWDAHGAPPALSRFLAEQGA
                     ALGRRVLVPGCGAAYEAVLLDAAGFDVLAIDYSAAAIARAREVLPADVAERVLRQADF
                     FAFDAAPFDWIYERAFLAALPPALWPRWSTRVAELLVTGGVLAGFFFIDDAVAEPRRG
                     PPFAARRAEVDALLDGFACEFQLEIARDQSAPVFAGREHWMTWRRTRSAAA"
     gene            complement(5012..>5327)
                     /locus_tag="AC16HB_06890"
     CDS             complement(5012..>5327)
                     /locus_tag="AC16HB_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606553.1"
                     /translation="TSKSIIVTWGERGRAGDPAVKPRAQQIASAFAPWIDAAQVDALA
                     APVSMRSGESWADNLVLSAGAQGARALGTQIDPRSDAELLQRALRRPGVGRTPSRQSA
                     QP"
BASE COUNT          926 a         1839 c         1747 g          815 t
ORIGIN      
        1 cgcttcgccg gcggccgcga atcgatcgac gagcgcgtca agcgctattc gaaccgggcc
       61 gaatcctggt tcggctccgt gaccgccgaa cagacgcaga tcctgcacgc tgcgctcgcc
      121 acagcgaccg aaacatggtg gctcgaagag cgcgagcggc gccagcacga gctgatgcgt
      181 ctgctgcggg ggctccatga cgagaagcca ggtgccgccg aggcgacgcg ccgcgtgcgc
      241 gactacttcg cgcagctcac cgagccccac gacgaggtgc gccgcgcgtc gctgctggaa
      301 tatcggcgct cgaatgccga gctgctggcg cgcctgatca acgccgccac gcccacgcag
      361 cgggccacgc tggcgaagcg gctgcgcaac tatgcggagg atttcacggc gctggccgac
      421 gcgagcaagg ggcgcggctg atccgcattc agcggatctt gatctcggtc cacagctgcc
      481 cgcgcagctg acgcagctcg gccgacagca gccgcagctg gtgcagcttc ttcaggctct
      541 ccgcgtcggg aaacaccgcg acgttcgact tcacctcggg cttgatgaat tccatcgccg
      601 cctgattggc gttgccggat ccgatcaggt tcgacagctc cgagctgttc tggccctcga
      661 gcatgaagtt gatgaatgcg tgcgccagcg ccgcgttcgg cgccgccttg tgaatcacca
      721 tgttgtcgac cgacagcacg gcgccctgcg tcggcagaac cgacacgatg cggaaatcct
      781 gcttcgcctc ctgcgcgttg cggttggcct ggaacatgtc cgtcgagtaa ccgagcgcca
      841 gccagatgtt gcccgccgtg agttccttga tgtacgaaga tgagttgaac gcagcccagc
      901 acggcttggc cttgcggatc gcctcggcgg ccttcttgta gtcgtcggcg ctggtcgtat
      961 tcggatcgat gccgaggtag atgaatgccg ccgagaacac ctcgtcgggc gagtcgagca
     1021 cggtcagctt gcccttcacg cggcaggcga tcttcgggtc gaagatcgcc tcccagcccc
     1081 tgggttcgat gccgagctgc ttcatgtgct tgtcgttgta gccgatgatc gtggtcgagt
     1141 acgcgtacgg caccgagaac ttgttgccgg gatcgatcgg cgtgttcagg aactccggcc
     1201 gaatattctt caggttcggc agctgcgcgg gatcgagcgg ctgcagatcg cccttcttga
     1261 tcatcagctc catcgcgttg ctggtcggca cgatgatgtc gtagccggtc gcgcccgcgg
     1321 cgagcttcgc cagcatctcg tcgttgtccg agaagtaggt ctgcatgacc ttggcgttga
     1381 acgccttctc gaatcgctcg atggtctcgg ccgcgatgta gtcgttccag ttgtacaggt
     1441 tcaatgtccc ggtcggcttc gccggcgcgg ccttcgcctc cgccttggcc tcggccttcg
     1501 gcggcggtgc ctcctctttc ttgccgcacg ccgccagaga gacggccgtc atcaccatcg
     1561 cgaaacgcag caggtcacgc agtttcatct tttcctcctt gccgaacgaa agctatcggt
     1621 tcaggcgcag cagcccgggc acgaacttgg tcgccagcgt gatcagcgcc agcgtcagcg
     1681 ccagcagcag cgtcgagatc gcattgacct cgggtgtcac cgcgaccttg accatcgagt
     1741 agatctgcag cggcagcgtc gaggccgacg ccccggcggt gaagaaagtg atcacgaaat
     1801 catcgatcga cagcgtgaag gccatcaggc tgcccgcgat cacgcccggc atgatcagtg
     1861 gcagcgtcac gtacctgaat gccgccggcg cggaggcacc gcagtcgcgc gccgcctcga
     1921 ccaggctttc gtccatgcct gcgagccgcg cgcgcacgac gatcgccacg tagccgatgc
     1981 agaacgccac gtgcgcgagc gtgatcgaca ccagcccgag cgtcaggttg atcatcacga
     2041 agaacatcag cagcgccaca cccatcagga tctcggggat cgcgatcggc gccagcacca
     2101 gcaccggcag cacgcgcgtg cggtagcggt gcatcacgaa tcctgccatt gtgccgagca
     2161 ctgtcgacgc cagcgacgtg acgagcgcga tcgcgagcga gttgcgcgct gcccgcatca
     2221 tctcctcgtt gtggaagagc ttcacgtacc acgcggtcgt gaagccgacc cactgcgcgt
     2281 tcagcttgct gtcgttgaac gagaacacca ccaccacgac cagcggcgcg tacaggaaca
     2341 cgtacgcagc cgccgcgcat gcccacatcc aggccgggac gcggcggttc atgggctgcg
     2401 ccgcggtcgc gcggcgagcg ccgccagcgc ggtgaccgcg agcgcggcca gcgtcaggac
     2461 gatcgacagc atcgagccga acggccagtc gcgcgtctcg aggaactgct gcttgatcac
     2521 gttgccgatc atgatgccgc tggtgccgcc gacgagatcc ggcaccgcga atatgccgaa
     2581 cgccgggatg aacaccagcg cggcgccggc aaacacgccc ggcagcgaca gcggccaggt
     2641 cacgtgccag aagcgctgcc acggccgcgc gcccaggtcc tgcgcggcgt cgagcagcgc
     2701 ggggtcgtgc ttctccaggt tcgcgtacag cggcagcacc atgaacggca ggtgcacgta
     2761 gacgagcacg acgatcaccg cgaacggcga gaacatcagc gtcaccggct cggcgccgaa
     2821 caatccgagc acgccgttga cgagccgcgc cagcgcccac tcgggtccga ggatgatcat
     2881 ccatgcatag acgcgaatca ggaagttgct ccagaacggc agaatcacga gcagcagcag
     2941 caggtcgcgg tacctcttcg agctcgcggc gatcagcatc gacagcggat acgcgatcgc
     3001 caggcacagc gcggtcgtca acagcgcata tgcgagcgat ctagcgaaca actccaggta
     3061 gatcgggctc gcgaagaacc gtgcgtaact ggcccaggtc aggttgagcc ggccgctgtc
     3121 ggtgaccagc ggcgccagcc cgccgaattc gccgggcgcg cgcacgctgg cgaccaccat
     3181 caacaggctc gggatcgcga agaagacgat cagatagacg aagggcggcc cgccgaccag
     3241 ccacttgccg cgctctgccg tggccacgtc agtcgaacag gaaatggccg gcgccgacct
     3301 gccaagcgac gtcgaccgcg tcgccgacct cgaagaagcg cgcgcggccc gcggccgaat
     3361 tcgcgcgcat ggcctcgatc ttccgtcccg acgccagcgc gacgcggtaa agcgtcacgt
     3421 cgcccaggta cagaaagtcg gtcaccgtgc ccgacacatg attgtcgccg tcggtcttcg
     3481 gctggccgct cgggttgatc tcgaccttct ccggccggag cgcgagcgcg ccgcgcacgc
     3541 cgctcgcgag cgccgcgtcg atcagcgcgc gcgccggccc gacgccgggc aggtcgagct
     3601 cggcgaccgt gccgtcgcgc cggcctacgg tcgcctcgag gatgttgcac tggccgatga
     3661 aatcggcgac gaagcggttc ttcggatacg agtagatcct cgagggctca tcgagctgct
     3721 cgatgcggcc ctgattcatc accgcgatcc ggtgcgacag cgcgagcgcc tcgccctggt
     3781 cgtgcgtcac gtagacgaac gtgatgccga cctccttctg cagattgatg agctcgatct
     3841 gcatctgttc gcgcagcttg gcgtcgagtg ccgccagcgg ctcgtcgagc agcatgagcg
     3901 ccggatgcgt gaccagcgcg cgcgcgatcg ccacgcgctg tcgttgaccg ccggacagtt
     3961 cgtgcggaaa gcgtgccgcc agcgcgtcga gctgcacgtc cgccagcgcc tcgcgcacct
     4021 tggcaccgag ttcgcccgcc ggcgtcttcg ccatcttcag cggaaacgcg acgttggcgg
     4081 ccaccgtcat gtgcgggaac agcgcataac tctggaatac ggtgcgcacg ttgcgccgct
     4141 cgggcggcac gccggtgagg tccttgccgt cgagcacgat gcgcccgcca tcggacgcat
     4201 cgaatccggc gatcatccgc agcaaggtcg tcttgccgca gccggagggc cccagcagcg
     4261 tgaagaactc gcccgcctcg atcgacagcg acacaccgtc gaccgcggtc atcgatccga
     4321 agcgacgggt gacgttctgg atttcgagca gggccatggc gggtcgaggg caggcgcgat
     4381 tatgcggcag cagacctcgt acgacgccag gtcatccagt gctcgcggcc cgcgaagacc
     4441 ggggccgatt gatcccgcgc aatttccagt tggaactcgc aagcgaagcc atccaacagc
     4501 gcatcgactt ccgcgcgacg ggcggcgaac ggcggtccgc gtcgcggctc ggcgaccgca
     4561 tcgtcgataa agaagaagcc ggcgagcacg ccgccagtca ccagaagttc agccacgcgc
     4621 gtcgaccagc gcggccacag cgcgggcggc agcgccgcga ggaatgcgcg ctcgtagatc
     4681 cagtcgaacg gcgccgcatc gaaggcgaag aagtccgcct gtcgcagcac gcgctcggcg
     4741 acgtcggccg gcagcacttc gcgcgcgcgt gcgatcgctg ccgccgagta gtcgatcgcc
     4801 agcacgtcga acccggcggc atcgagcagc accgcctcgt acgccgcgcc gcagcccggc
     4861 accagcacgc gccgtccgag agcggcgccc tgctcggcca ggaagcgcga caacgccggc
     4921 ggtgcgccgt gcgcgtccca cggcgtgaag ccggcggcaa agcgctcgtc ccagaactcg
     4981 ggcctcgagg cgtcgcgcgt ggtgaagccg gtcatggctg cgccgattgc cgcgacggcg
     5041 tgcggccaac gcccggccga cgcagcgccc gctgcagcag ctcggcgtcg ctgcgtggat
     5101 cgatctgcgt gcccagggcg cgcgcgccct gggctccggc gctgagcacc aggttgtccg
     5161 cccagctctc gcccgaacgc atcgaaaccg gcgcggccaa cgcgtcgact tgcgcggcgt
     5221 cgatccacgg cgcgaatgcc gacgcgatct gctgcgcgcg cggtttgacc gccggatcac
     5281 ccgcgcgtcc gcgctcgccc caggtcacga tgatcgactt cgacgtg
//
LOCUS       DBNHBY010000280         4643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_287610, whole genome shotgun sequence.
ACCESSION   DBNHBY010000280 DBNHBY010000000
VERSION     DBNHBY010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_287610"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16HB_06895"
     CDS             <1..784
                     /locus_tag="AC16HB_06895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5606554.1"
                     /translation="HRFPDGELKLTLPPALPPRVVALRSLHQPNEKLIELMLVARTAR
                     ELGARELVLVAPYLGYMRQDIAFNPGEAVSQRIVGKFLASLFDTVIAVDPHLHRTGEL
                     GAAVPASRVIAVATAPLVAPFLARREDDPILIGPDEEAAQWVRPAAAGRYAWTVCRKT
                     RNGDRDVEIVLPGMDVRGRDVVLIDDIASTGRTLVQTVHGLWDAGATGIDVFVTHPVF
                     VGDALAQLRAAGVGSIWSCDTVPHPTNAISVVPLLAEALRTL"
     gene            complement(861..1241)
                     /locus_tag="AC16HB_06900"
     CDS             complement(861..1241)
                     /locus_tag="AC16HB_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HIR5606555.1"
                     /translation="MATAKKAKKATAKKAKKAAPKKAAAKKTVKKAAPKKAAPKKKVA
                     RKPNPAFMRPLMPTGPLAAIIGSAPMPRTEVTSKIWAYIKKNKLQDAVNRRMINADEK
                     LRALFGKAQASMFELTKYVSKHLK"
     gene            complement(1856..4534)
                     /locus_tag="AC16HB_06905"
     CDS             complement(1856..4534)
                     /locus_tag="AC16HB_06905"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HIR5606556.1"
                     /translation="MTRLPPGPNVGTQLRAIRARQRRAERALAELPAPAELARRPPPL
                     QLALPSAEAWFAAHRWRPFDFQREVWAHVAAGRSGLLHATTGSGKTYAVYFALLNRAL
                     SGEVPGGGLRLLWVTPMRALSADTARALAAPLGSLGLPWQVGVRTGDTEGAERARQDR
                     RLPEVLVTTPESLTLLLTRTDCREQFAGLALVVVDEWHELIGNKRGVQVQLALARLRR
                     FARERAVQSAAAPRPRAEAVGGGTGSRGLPDPQTQLSVWGLSATLGNLDEAMRALLSH
                     GDGVLVRGRIDKSIVVDTLLPDDPGRFPWGGHLGIRMLDPVLREIAASGTTLVFTNTR
                     SQAEIWYQSILGARPQWAGLIALHHGSLDREVRDWVERGLKEGRLKAVVATSSLDLGV
                     DFLPVERVLQIGSPKGMARLLQRAGRSGHAPGRVSRVTLVPTNTLELIEAAAAQKAAA
                     TGVRDGRVESRHAPAKPLDVLVQHLVTIALGTGFTEQDLLAEVRDTASFATLTDAEWH
                     WALDFAGRGGAALHVYPQYRRVAADADGVFRVPDAQVARRHRMSVGTIVAEASMLVRY
                     GNGARLGFIEENFIARLNPGDTFTFAGKLLELVRVQDMTAFVAPSKRKSGAVPRWAGT
                     KMPLSSEMAEAVLQELEGALAGCYDTAELRLARPLIELQARWSAVPTRSDLVVESLRS
                     REGRHLFVYPFAGRLAHQGLAALLAVRAARSAPSSFSLAVNDYGLELLATADLDWRTL
                     WRHGGLLDEANLLEDILSSLNAGQLAQRRFREIARVAGLIFQGYPGQPKSTRQLQASS
                     QLIYEVFRKYDAGNLLLTQAEREVLEQELDLARLRTALARMRAQRLVYRNLLQPTPFA
                     FPLMVERLREELTTEKLADRIARMVAELERQASGAG"
BASE COUNT          676 a         1654 c         1568 g          745 t
ORIGIN      
        1 ccaccgcttt cccgacggcg aactgaagct caccctgccc ccggcgctgc cgccgcgcgt
       61 ggtcgcgctg cgctcgctgc accagccgaa cgagaagctc atcgagctga tgctcgttgc
      121 gcggaccgcg cgcgaactgg gcgcgcgcga actggtgctg gtcgcgccgt acctcggtta
      181 catgcggcag gacatcgcgt tcaaccccgg cgaggcggtc agccagcgca tcgtcgggaa
      241 gttcctggca tcgcttttcg acaccgtgat cgcggtcgat ccgcacctgc accgcaccgg
      301 cgaactcggc gcggcggtgc cggcttcacg cgtcatcgcg gtggccaccg cgcctctggt
      361 ggcgccgttc ctggcgcgac gcgaggacga tccgatcctg atcggccccg acgaggaggc
      421 ggcgcaatgg gtgcgcccgg cggcagccgg acgctacgcg tggaccgtgt gccgcaagac
      481 gcgcaacggc gaccgcgacg tcgagatcgt gctgccgggg atggacgtgc gcggccgcga
      541 cgtggtgctg atcgacgaca tcgccagcac cggccgcacg ctggtgcaga ccgtgcacgg
      601 cttgtgggac gcgggcgcca ccggcatcga cgtgttcgtc acgcacccgg tgttcgtcgg
      661 cgacgcgctc gcccagctgc gcgcggcggg tgtcggcagc atctggagct gcgatacggt
      721 tccgcatccg accaacgcga tctcggtcgt gccgctgctg gcggaagcgc tgcgcaccct
      781 gtagaaacga aaaaacccgc gcgtggtgcc accgcgcggg tatctgaccg tcggccaccc
      841 ttggaccgac ccgggcagct ctatttcagg tgtttgctga cgtacttggt cagctcgaac
      901 atcgacgcct gcgccttgcc gaacagcgcg cgcagcttct cgtcggcgtt gatcatgcgc
      961 cggttgaccg cgtcctgcag cttgttcttt ttgatgtacg cccagatctt gctggtcacc
     1021 tcggtgcgcg gcatcggcgc gctgccgatg atcgccgcca gcgggccggt cggcatcagc
     1081 ggcctcatga aagccgggtt cggcttgcgt gcgactttct tcttcggtgc ggccttcttc
     1141 ggcgcggcct tcttgacggt cttcttcgcg gcggctttct tcggcgccgc tttcttggcc
     1201 ttcttcgcag ttgctttctt cgccttcttg gcagttgcca ttgaacgatc tcctgaactg
     1261 gtttcgatgg gaccacagcg acagcgctct cctctgaaac cacatcgaga gcgctctcgt
     1321 tgcgtgacgc ggatactaag cgaaaaaagt cgcgatgcaa gggttttcag agggaaattt
     1381 gcgtttcacc cgtgtgcgcg gcacgcattc tcagagggaa aaccgcgtct ccgaggcccc
     1441 acggcgcgga tcatgcgtca cgcgacccct acaagatatt gatcgagaag cgatgggctg
     1501 ctcgatatgt tgggccgctt gtggtgcgtg gcgggcctcg atcgcggccg tttctgccgc
     1561 gaccgccgag cggataaccc attaccgcgc agtcgcggga acgcgacgcg cacgagcgac
     1621 acactggtcc cgtccgctgg gcttgcgttc gatccgtgcc gcgagcttct gtctccggcc
     1681 gcgccgctgc ggcggcgctt aaccgcggct gacgccgcag ttggcctcga cgaaagctcg
     1741 catcaggccg cggcagactg ctccagttcc ctcagcatgc gttcgttccg tgcggcgagc
     1801 ttttggctac ggccgcagcg ctgttcgcgc tgcttaacgt cggcagtcgc cgccgttagc
     1861 ctgcgccgga agcttgccgc tcgagctcgg ccaccatgcg tgcgatccgg tcggcaagct
     1921 tctcggtcgt cagctcctcg cgcaggcgct cgaccatcag ggggaacgcg aacggcgtcg
     1981 gctgcagcag gtttcggtag acgagccgct gcgcgcgcat gcgcgccagc gccgtgcgca
     2041 gccgcgccag atcgagttcc tgctccagta cttcgcgctc ggcctgggtc agcagcaggt
     2101 tgccggcgtc gtacttgcga aacacctcgt agatcaactg actcgacgcc tgcagttggc
     2161 gcgtcgactt cggctggccc gggtagccct ggaagatcag ccccgccacg cgcgcgatct
     2221 cgcggaagcg gcgctgcgcg agctggcccg cgttcaggct ggacaggatg tcctcgagca
     2281 ggttcgcctc gtcgagcaat ccgccgtggc gccacagcgt gcgccagtcg agatctgccg
     2341 tcgcaagcag ttccagtccg tagtcgttga ccgcgagcga gaacgacgac ggcgcgctgc
     2401 gcgccgcgcg caccgcgagc agcgcggcca gcccctggtg tgcgagccgg ccggcaaatg
     2461 gatagacgaa caggtgccgg ccctcgcgtg agcgcagcga ctcgaccacg aggtcgctgc
     2521 gggtcggcac cgcggaccag cgcgcctgca gttcgatgag cgggcgcgcg agccgcagct
     2581 ccgcggtgtc gtagcagccg gccagcgcgc cttcgagctc ctgcagcacg gcctccgcca
     2641 tttcggacga cagcggcatc ttcgtgcccg cccagcgcgg taccgcgccg ctcttgcgct
     2701 tcgacggcgc cacgaacgcg gtcatgtcct gcacgcgcac cagttccagc agcttgccgg
     2761 cgaacgtgaa ggtgtcgccc gggttcaggc gcgcgatgaa gttctcctcg atgaagccga
     2821 gcctggcgcc gttgccgtaa cggaccagca tcgaggcctc ggcgacgatc gtgccgaccg
     2881 acatccggtg ccgccgcgca acctgcgcat cgggcacgcg gaacacgccg tcggcatccg
     2941 ccgccacccg ccggtactgt ggatacacat gcagcgccgc gccgccgcgg ccggcgaaat
     3001 cgagcgccca gtgccactcg gcgtcggtga gtgtcgcgaa gcttgcggtg tcgcgcactt
     3061 cggccagcag atcctgctcc gtgaagccgg tgccgagcgc gatcgtcacg aggtgctgga
     3121 ccagcacgtc gagcggcttg gcgggagcat ggcgcgactc gacacggccg tcgcgcacgc
     3181 cggtcgcggc cgccttctgc gcagcggcgg cctcgatcag ctccagcgtg ttggtcggca
     3241 ccagtgtcac gcgcgacaca cgccccggcg cgtgcccgct gcggccggcg cgttgcagca
     3301 gccgcgccat gcccttcggc gagccgatct gcagcacgcg ctcgaccggc aggaagtcga
     3361 caccaaggtc gaggctgctg gtcgcgacca ccgccttcaa tcgcccttcc ttcagcccgc
     3421 gctcgaccca gtcgcgcacc tcgcgatcga gcgaaccgtg atgaagcgca atcaaaccgg
     3481 cccattgcgg ccgcgccccg aggatcgact gataccagat ctccgcctgc gagcgcgtgt
     3541 tggtgaacac gagcgtggtg ccgctggccg cgatctcgcg cagcaccgga tcgagcatcc
     3601 tgatgccgag gtgcccgccc caggggaagc ggcccgggtc gtcgggcagc agggtatcga
     3661 ccacgatcga cttgtcgatc cggccgcgca ccagcacacc gtcgccgtgg gagagcagcg
     3721 cgcgcattgc ctcgtcgagg ttgccgagcg tggcgctcaa gccccacacc gagagctgcg
     3781 tttgcgggtc gggcaggccc cgagatcctg tccctccacc cacggcctcc gccctcggac
     3841 gcggggcggc ggcgctttgc accgcgcgct cgcgcgcgaa gcgccgcagg cgcgcgagcg
     3901 cgagctggac ctgcacgccg cgcttgttgc cgatcagttc gtgccattcg tcgacgacga
     3961 ccaacgccag cccggcgaac tgctcgcggc aatcggtgcg cgtcagcagc agcgtcaggc
     4021 tttcgggggt ggtgaccagc acctcgggca ggcgcctgtc ctggcgcgcg cgctccgcgc
     4081 cctccgtgtc gccggtgcgc accccgacct gccacggcag cccgagcgag ccgagcggcg
     4141 ccgccagcgc gcgtgcggtg tcggcgctca atgcgcgcat cggcgtcacc cacagcaacc
     4201 gcaggccgcc gcccggcact tcgccggaca gcgcgcgatt cagcagcgcg aagtacaccg
     4261 cgtaggtctt gcccgagccg gtggtcgcgt gcagcagccc cgagcgaccg gcagcaacgt
     4321 gcgcccatac ctcgcgctgg aaatcgaacg gccgccagcg atgcgccgcg aaccatgctt
     4381 ccgcgctcgg cagcgccagt tgcagcggcg gcgggcgacg cgccagttcg gccggcgccg
     4441 gcagctcggc cagcgcccgc tccgcccgcc gttggcgcgc gcggatcgca cgcagctggg
     4501 taccgacgtt ggggccgggc ggcagccgag tcatgtgcgg atgatgcccg cgcaagaaaa
     4561 acgccgccct cgggcggcgt tcttcgtcga agcgtgcgcg gctgttactg gacgccgaga
     4621 atcttcatgg ccttggccag cgt
//
LOCUS       DBNHBY010000281         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1437388, whole genome shotgun sequence.
ACCESSION   DBNHBY010000281 DBNHBY010000000
VERSION     DBNHBY010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1437388"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC16HB_06910"
     CDS             complement(<1..738)
                     /locus_tag="AC16HB_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5606557.1"
                     /translation="MAQQPSVVLLHASASSARQWSALGEMLRPRFRVRALDLHGHGAR
                     PAWRGDAPFTLADDAALAAPLLDQAGAVHLVGHSYGAAVALKLATLHPRSVRSVVVYE
                     PVLFRWLIDDPARHHHPVQDVIAVATSMRERHARGESRAAAQRFVDFWSGAGAWDALS
                     VEAWDAIAQRVPALLRQFDALFREPMPRARLSGLDVPMLFLSGARTIAVARRIADLLR
                     AALPDAGHDILPQMGHMGPITHAAEVNS"
     gene            complement(803..1771)
                     /locus_tag="AC16HB_06915"
     CDS             complement(803..1771)
                     /locus_tag="AC16HB_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606558.1"
                     /translation="MQTTVADPFRHCAITAAQRYARCVEASRRIRWDIDRDVIRGRSL
                     EFDRKFMPDALSKLDELPFLQPDERLLLSQIQGRTYANMLALLERFIGAKTLEISRDH
                     WLGDQVALEALVRFADEELKHQALFRRLEAIAAKRMPGGYAFRPDANEVAAAVLRRST
                     WAALGLTLDIELASQAHYRSSIESAQDLSELWQDVFLFHWKEEAQHAVLDELEWRRED
                     ARLLDVERDRGVDDLIELVAVVDDIVQSQARADADYFLRCAGRSFSPAEANAIGDVML
                     KAYRWQYIASGMQEPRFVAVLKTLASPRQMERIGIALAPIVAHTES"
     gene            complement(1810..>2302)
                     /locus_tag="AC16HB_06920"
     CDS             complement(1810..>2302)
                     /locus_tag="AC16HB_06920"
                     /inference="COORDINATES: protein motif:HMM:NF018294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HIR5606559.1"
                     /translation="PGASDGVGGVCPLLPSTTGSLRDVAVTAAGADDAPERDEIEAYA
                     LRVIGANGFGDAALAHTEPHTLRPSGHLGRAARVHAARRPGAVFASALRACAAWVRGR
                     VAAWQRRRRVRETYRALLALDEHTLRDLGIDRTEALSVASELAGDAEATRLHPERVLR
                     GLY"
BASE COUNT          360 a          808 c          808 g          326 t
ORIGIN      
        1 cgagttgact tccgcggcgt gggtgatcgg ccccatgtgg cccatctgcg gcaggatgtc
       61 gtggccggcg tccggcagcg cggcgcgcag cagatcggcg atgcggcgtg cgaccgcgat
      121 cgtgcgcgca cccgacagga acagcatcgg cacgtcgagg ccggacagtc gtgcccgcgg
      181 catcggctcg cggaacaggg cgtcgaactg ccgcagcagc gccggcacgc gctgtgcgat
      241 cgcgtcccac gcctccacgg acagcgcatc ccaggcgccg gcgccggacc agaagtcgac
      301 gaagcgctgc gcggcagcgc gagactcgcc gcgcgcatgt cgctcgcgca tcgacgtggc
      361 gacggcgatc acgtcctgca ccggatgatg gtggcgcgcg ggatcgtcga tcagccagcg
      421 gaacaaaacc ggctcgtaca cgaccacact gcgcaccgag cgcggatgca gcgtcgcgag
      481 cttcagtgcc acggccgcgc cataggagtg gcccaccagg tgcaccgcgc cggcctgatc
      541 cagcagcggc gccgcgagcg cggcatcgtc ggccagcgtg aacggcgcat cgccgcgcca
      601 tgcgggccgt gcaccgtggc cgtgcagatc gagcgcgcgg acgcggaagc gtgggcgcaa
      661 catctcaccg agcgcgctcc attgccgcgc cgaactcgcg ctcgcgtgca gcagcacgac
      721 cgacggctgc tgcgccacag gggtcagctt gtcagaagag caacacattc agtctccgtg
      781 agaacgtcgt ccaggggtgc ggtcaggatt cggtgtgggc gacgatcggc gccagcgcga
      841 tgccgatgcg ctccatctgc cgcggtgaag cgagtgtctt cagcaccgcg acgaaacgcg
      901 gctcctgcat gccgctggcg atgtactgcc agcggtatgc cttcagcatc acgtcgccga
      961 tcgcattcgc ttcggccggc gagaacgacc ggccggcgca ccgcaagaag tagtccgcgt
     1021 cggcccgcgc ctgcgattgc acgatgtcgt cgaccacggc gacgagctcg atcaggtcgt
     1081 ccacgccgcg gtcgcgctcg acgtcgagca accgcgcgtc ttcgcgccgc cactccagct
     1141 cgtcgaggac cgcgtgctgc gcttcctcct tccagtggaa caggaacacg tcctgccaca
     1201 gctccgacag gtcttgtgcg gactcgatgc tcgaccggta gtgcgcctgc gaggcgagct
     1261 cgatgtcgag tgtcagcccg agcgcggccc aggtcgaccg gcggagcacg gctgcggcga
     1321 cttcgttcgc atcgggccgg aacgcgtagc cgcccggcat ccgcttcgcg gcgatcgctt
     1381 ccagccgccg gaacagtgcc tggtgcttca gttcctcgtc ggcgaagcgc accagtgctt
     1441 cgagggcgac ctggtctccg agccagtggt cccggctgat ctcgagcgtc ttggcgccga
     1501 tgaagcgctc gagcagcgcg agcatgttcg cgtacgtgcg cccctggatc tgcgacagca
     1561 gaagcctttc gtccggctgc aggaacggca gttcgtcgag cttcgacagc gcgtccggca
     1621 tgaacttgcg gtcgaactcc aggctgcggc cgcggatcac gtcgcgatcg atatcccagc
     1681 ggatgcgccg ggacgcctcg acgcaacgcg cgtagcgctg cgcggccgtg atcgcgcagt
     1741 gtcgaaaagg atcggcgacg gtggtttgca tggcggtatg tctcgcgacg gcggtcggcg
     1801 gcaatcgctt cagtacaggc cgcgcagcac ccgctccggg tgcaggcggg tggcctccgc
     1861 atcccctgca agttcggagg cgaccgacag tgcttcggtg cggtcgatgc cgaggtcccg
     1921 cagcgtgtgt tcgtcgagtg cgagcagcgc gcgataggtc tcccgcacgc gccggcgccg
     1981 ctgccatgcg gcgactcggc ctcgcaccca agccgcgcaa gcgcgcaggg cagacgcgaa
     2041 gaccgcgccc ggccgacgcg ccgcatgcac gcgcgcggcc cgtccgagat gaccgctggg
     2101 ccggagagtg tgcggctcgg tgtgcgccag cgcggcatcg ccgaagccgt tggcaccgat
     2161 gacgcgcagt gcatacgcct cgatctcgtc gcgctccggc gcgtcgtccg cgccggcagc
     2221 cgtgacggca acgtcacgca gcgaacccgt ggtcgatggc agcagcggac aaacgccgcc
     2281 gacaccgtcg gaggccccgg gc
//
LOCUS       DBNHBY010000282         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_83813, whole genome shotgun sequence.
ACCESSION   DBNHBY010000282 DBNHBY010000000
VERSION     DBNHBY010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_83813"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="AC16HB_06925"
     CDS             complement(<1..1253)
                     /locus_tag="AC16HB_06925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5606560.1"
                     /translation="MEKKTPFNIAYLIFAIVAVLILQNLWVGLRAIEPMAYSEFVAQL
                     KSGNVDEIAVAANVIQGTLKTPLADGRKQFVTTRVDPDLARDLEQYNVKFSGVIENTF
                     LKDLLGWVVPTLLFFGIWFFLVRRMQERGGIGAGLLSIGKSKAKVYVETDVKVTFEDV
                     AGVDEAKEELREVVGFLKDPHKYGRLGARIPKGVLLVGPPGTGKTLLARAVAGEAGVP
                     FFSINGSEFVEMFVGVGAARVRDLFEEGRKKAPAIIFIDEIDALGRARGAFGLGGHDE
                     KEQTLNQLLAELDGFDPRSGLVLLAATNRPEILDPALLRAGRFDRQVLVDRPDRVGRV
                     QILKVHVRRIKIAPGVDLDQIAGLTPGFSGADLANLVNEAALQATRRGRDVVGQDDFT
                     LAIERIVAGLEKRTRILNPKERAVVA"
     gene            1421..1966
                     /locus_tag="AC16HB_06930"
     CDS             1421..1966
                     /locus_tag="AC16HB_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5606561.1"
                     /translation="MSWKSTRTRYGLVPIALHWLMLLLLAAVCATMELKSIFPRGSAQ
                     RDALAAWHYLLGLSVFVLVWLRLLARAAGTAPLIAPPPPPWQAMLARLAHGALYALMI
                     MLPLLGWLTLSARGTPVTYLGVELPALVGANKPLAGWLKEIHEALATAGYFVVGLHAA
                     AALYHHYVRHDNTLRQMLPVR"
     gene            1978..>2681
                     /locus_tag="AC16HB_06935"
     CDS             1978..>2681
                     /locus_tag="AC16HB_06935"
                     /inference="COORDINATES: protein motif:HMM:NF020811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIR5606562.1"
                     /translation="MAAHPAEPAGATRAAALDYAARGWSVIPIEPRGKRPLVTWLEFQ
                     QHCADAVQIDAWFARWPDANVGIVTGSVSRLAVIDVDAQHGGLQSLATLEDEHGALPR
                     TVEAVTGGGGRHLYFTHAQPPLHNRVGIRPGIDLRAEGGCVVAPPSVHPSGRRYAWAS
                     GCAPDQVSLAPLPRGLFELMRPAAHGGHGLAHWRHLVLAGVAEGERNATLASLTGHLL
                     WHGIDPQVALELLLAW"
BASE COUNT          385 a          930 c          930 g          436 t
ORIGIN      
        1 gcgaccaccg cccgctcctt cgggttcagg atgcgcgtgc gcttttcgag cccggcaacg
       61 atgcgctcga tcgccagcgt gaagtcgtcc tgtcccacga cgtcccggcc gcgccgcgtg
      121 gcctgcagcg ccgcctcgtt gacgagattg gcgagatcgg cgcccgagaa gcccggtgtc
      181 agcccggcga tctgatccag atcgacgccg ggtgcgatct tgatcctgcg cacgtgcacc
      241 ttgaggatct gcacgcggcc gacgcggtcg ggccggtcga ccagcacctg ccggtcgaag
      301 cgcccggcgc gcagcagcgc cggatcgaga atctcgggcc ggttggtcgc cgccagcagc
      361 acgaggccgc tgcgcgggtc gaagccgtcc agctccgcca gcaactggtt tagcgtctgc
      421 tccttctcgt cgtggccgcc gagaccgaac gcgccgcggg cgcggccgag cgcgtcgatc
      481 tcgtcgatga agatgatcgc cggcgccttc ttgcgcccct cctcgaagag atcgcgcacg
      541 cgcgcggcgc cgacgccgac gaacatctcg acgaactcgg agccgttgat cgagaagaac
      601 ggcacacccg cctcgccggc gacagcgcgc gcgagcagcg tcttgccggt gccgggcggg
      661 ccgaccagca gcacgccttt cggaatgcgc gcgcccagcc ggccatactt gtgggggtcc
      721 ttgagaaatc cgacgacctc gcgcagttcc tccttcgcct cgtccacacc cgcgacgtct
      781 tcgaacgtga ccttgacgtc ggtctcgaca tacaccttgg ccttgctctt gccgatcgac
      841 agcagcccgg cgccgatgcc gccgcgctcc tgcatgcgtc gcacgaggaa gaaccagatg
      901 ccgaagaaca gcagcgtcgg cacgacccag cccagcaggt ccttcaggaa cgtgttctcg
      961 atcacgccgc tgaacttgac gttgtactgc tcgaggtcgc gcgcgaggtc cggatcgacg
     1021 cgtgtggtga cgaactgctt gcggccatcg gcgagcggcg tcttcagcgt gccctggatc
     1081 acgttggccg cgaccgcgat ttcgtcgacg ttgccgctct tcagctgggc gacgaattcg
     1141 ctgtacgcca tcggctcgat cgcgcgcagg ccgacccaca ggttctgcag gatcagcacc
     1201 gcgacgatcg cgaagatcag gtacgcgatg ttgaacggag tcttcttttc cacgctgcct
     1261 tcggtgtcgc attggccgcc cgttcgggcg gcggggcatc cgctggcttg acgatagccg
     1321 cgcgggccgc cgcggccatg acgaaacgca atcgcgcccg gcccgccgcg cgcaccattt
     1381 tgcgtaggtg ggatctaact accgggaggg tgagttgcgt atgtcctgga agagcacacg
     1441 aacacggtac ggacttgttc cgatcgcact gcactggctg atgctgctgc tgctggcagc
     1501 ggtgtgcgcg acgatggagc tgaagtccat ctttccccgg ggcagcgcgc agcgcgacgc
     1561 gctggcggcg tggcactacc tgctcggctt gtcggtgttc gtcctcgttt ggctgcggct
     1621 gctcgcgcgc gcagcgggca ccgcgccgct gatcgcacca ccgccgccac cgtggcaggc
     1681 gatgctggca aggctcgcgc acggcgcgct gtatgcgctg atgatcatgc tgccgctgct
     1741 cggctggctg acgctgagcg cgcgcggcac gcccgtgacg tatctcggcg tcgaactgcc
     1801 tgccctcgtc ggcgcgaaca agccgctggc ggggtggctg aaggaaatcc acgaagcgct
     1861 cgcgacggcg ggctatttcg tggtcggcct gcacgcggcc gccgcgctgt atcaccacta
     1921 cgtgcgccac gacaacacgc tgcggcagat gctgcccgtg cgctgaaggg tgcagccatg
     1981 gccgcgcacc cggcggaacc ggcgggcgcc acgcgcgccg cggcgctcga ttacgcggcg
     2041 cgcggttggt cggtgatccc gatcgagccg cgcggcaagc gcccgttggt gacctggctc
     2101 gaattccagc agcattgcgc cgacgccgtg cagatcgacg cgtggttcgc acgctggccc
     2161 gatgcgaacg tcggcatcgt gaccggttcg gtgtcgcggc tcgccgtgat cgacgtcgat
     2221 gcgcagcacg gcgggctgca aagcctggcc acgctcgagg acgagcacgg cgcgctgccg
     2281 cgcacggtcg aggcggtcac cggaggcggc ggacggcacc tgtacttcac gcatgcgcag
     2341 ccgccgctgc acaaccgcgt cgggatccgg cccggcatcg acctgcgcgc cgagggtggc
     2401 tgcgtggtgg cgccgccctc cgtgcatccg agcgggcggc gttacgcctg ggcgtcgggg
     2461 tgcgcgccgg atcaggtgtc gttggcgccg ctgccgcgcg ggttgttcga actgatgcgc
     2521 cccgccgcgc atggcgggca tggactggcg cactggcgcc atctcgtgct tgcgggcgtg
     2581 gccgagggcg agcgcaacgc gacgctggca tcgctgaccg gccatctgct gtggcacggc
     2641 atcgacccgc aggtcgcgct cgaactgctg ctggcgtgga a
//
LOCUS       DBNHBY010000283        12208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1302874, whole genome shotgun sequence.
ACCESSION   DBNHBY010000283 DBNHBY010000000
VERSION     DBNHBY010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1302874"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /gene="mobB"
                     /locus_tag="AC16HB_06940"
     CDS             complement(<1..250)
                     /gene="mobB"
                     /locus_tag="AC16HB_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR5606563.1"
                     /translation="MKAIGIIGYSNTGKTTLIEKMIPIFRARGLAVSAIKNAHHGFDM
                     DRPGKDSYRYREAGAGQVLIATGLRWALLTETPRHAATL"
     gene            complement(247..765)
                     /locus_tag="AC16HB_06945"
     CDS             complement(247..765)
                     /locus_tag="AC16HB_06945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606564.1"
                     /translation="MLELDLGRQLGWLNQPHVNRWWSRRRFTRDEVRAKYLPRVRGEQ
                     PIVCMIALLDARPAGFVQTYRIEDYAQYGPGFVLPRGGWGLDWFIGEPALIGRGLGAA
                     LLDAFVREWLWARGDAAYAVAGAHEDNVPATKCYESARFVHWFSTIPRGSSERYYRRD
                     RGSDEGWSNKKR"
     gene            complement(782..2191)
                     /gene="thrC"
                     /locus_tag="AC16HB_06950"
     CDS             complement(782..2191)
                     /gene="thrC"
                     /locus_tag="AC16HB_06950"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5606565.1"
                     /translation="MRYVSTRGKMPPAGFTDILLEGLAPDGGLVVPEFYPRIDRATLA
                     RWRALDYAALAAEVLSHYCTDIPRADLDRLTRATYTRATYGTDAITPLTKVAPGLHLL
                     HLSNGPTLAFKDMAMQLLGALFEYALAQRGERLNVLGATSGDTGSAAEYAMRGRAGIN
                     VFMLSPHGRMSAFQRAQMYSLQDPNIFNIAIEGVFDDCQDIVKKVSADLDFKRRYRIG
                     SVNSINWARVVAQVVYYFRGWFAATQNEDERVSFCVPSGNFGNILAGWIAKQMGLPVE
                     RLILATNENNVLDEFFRTGRYRVRSSRETHATSSPSMDISKASNFERYVFDVVGRDPD
                     AVRELWWQVDTRGEFDLAATPFWDKVRASGFVSGSSTHADRLRVIREVYESTGTIVDP
                     HTADGIKVAREHLDARMPTVCLETALPVKFAETIVEAIGRAPGRPAAYEGIEQRPQRC
                     VVLPDDALRVKDYIVAHAQ"
     gene            complement(2192..3502)
                     /locus_tag="AC16HB_06955"
     CDS             complement(2192..3502)
                     /locus_tag="AC16HB_06955"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5606566.1"
                     /translation="MKPIKVGLLGLGTVGRGVFNVLRRNQHEITRRAGRGIEIAAIAV
                     RNVHKAHDAIGTTVPITANAAEVIRRDDIDIVVELIGGYEPARTYVLDAIARGKHVVT
                     ANKALLARHGNEIFEAARAQGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNFILSEMRSKGLPFDAVLKEAQRLGYAEADPTFDIEGIDAAHKLTILSSIAFGVPI
                     NFDAAYTEGISKLAAADIRYAEQLGYRIKLLGITKRTDHGIELRVHPTLVPMQRLLAN
                     VEGAMNAVLVKGDAVGTTLYYGKGAGAEPTASAVVADLVDVTRMHTADPENHVPHLAF
                     QAEAMVEVPWLSIDDTVTSYYFRMRVADQPGVLADITRILADRRISIDALFQREPSEG
                     ENQTDIIMLTHDAVERDVNAAIRRIEALPTVLASIIRIRQEELI"
     gene            complement(3561..4760)
                     /gene="alaC"
                     /locus_tag="AC16HB_06960"
     CDS             complement(3561..4760)
                     /gene="alaC"
                     /locus_tag="AC16HB_06960"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR5606567.1"
                     /translation="MVEFPRIKRLPPYVFNITGELKMAARRRGEDIIDMSMGNPDGPT
                     PKHIVDKLVEAAQRPDTHGYSVSKGIPRLRRSITHWYKTRFAVDFDPDSEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTREVDFFAELERAIR
                     ESFPKPKMMILGFPSNPTAQCVDLDFFHHVVALAKQHGIYVVHDLAYADIVFDGFRAP
                     SIMEVPGARDVAVEFFTMSKSYNMAGWRIGFMVGNKDLVSALARIKSYHDYGTFTPIQ
                     VASIAALEGPQDCVEEVRAQYQRRRDVLAKGLQEAGWMVEVPKASMYIWAEIPPPYKP
                     MGSLEFAKKVLSEAKVAVSPGIGFGDYGDDHVRFALIENEHRIRQAVRGIKEMFRRDG
                     LLPRAAA"
     gene            4891..5265
                     /locus_tag="AC16HB_06965"
     CDS             4891..5265
                     /locus_tag="AC16HB_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR5606568.1"
                     /translation="MKLHADALNSLNTVTAYGPGFVEINQVRHRGNLILVPEQAVAAW
                     SAVSFEALRVEDFDALLELRPEVVLLGTGARQRFPHPRLTRNLAGAHIGVEAMDTRAA
                     CRTYNILMNEGRRVVAVFLQDD"
     gene            5274..5735
                     /locus_tag="AC16HB_06970"
     CDS             5274..5735
                     /locus_tag="AC16HB_06970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5606569.1"
                     /translation="MAVALDKKVPDFGAASTAGEFKLSALKGRSVVLYFYPKDNTPGC
                     TTEGSDFSVAYARFRKAGAEVLGVSRDSLKSHENFKAKMKFPFELLSDPDEKLCTLFG
                     VIKMKNMYGKQVRGIERSTFVIDGEGRLRREWRGVKVPGHVDEVLAFVQSL"
     gene            5771..7390
                     /locus_tag="AC16HB_06975"
     CDS             5771..7390
                     /locus_tag="AC16HB_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR5606570.1"
                     /translation="MPLPKPPSKPATLLTSIPTAAATPRKDTANVRPQATEIHSAPQA
                     RAPSSPAAEQRASAAAHLALVEARPKNGKKPRVSGAARRLFVLDTNVLMHDPTSLFRF
                     EEHDVFLPMMTLEELDSHKKGMSEVARNARQVSRSLDSLVAACKTPIEDGIPLDALGN
                     VDARGRLFFQTQAAAGPLPATLPAGKADNQILGVVKALQDTHPGRDVVLVSKDINMRV
                     KARALGLPAEDYYNDKVLEDTDILYTGVLALPANFWDKNGKGMESWQQSGYTFYRITG
                     PIADSLLVNQFVYLEDGAPFHAQVREIRSKTAVLRVLRDYQHAKNAVWGITARNREQS
                     FALNLLMDPECDFVTLIGQAGTGKTLLALAAGLAQTLETKRFSEIIVTRATVPVGEDI
                     GFLPGTEEEKMAPWMGALEDNLEVLNKSDDSAGEWGRAATNDLIRTRIRIKSLNFMRG
                     RPFIHKYLIIDECQNLTPKQMKTLITRAGPGTKVICLGNIAQIDTPYLTEGSSGLTFV
                     VDRFKGWPHSGHVTLQRGERSRLADHAAEVL"
     gene            complement(7449..8567)
                     /locus_tag="AC16HB_06980"
     CDS             complement(7449..8567)
                     /locus_tag="AC16HB_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5606571.1"
                     /translation="MKKALLGVAVGLVFAGTAWSQEVEVKIGHVAPLSGGQAHYGKDN
                     ERGAIMAIDDLNAQNLTIGGKKIKWKLISEDDAADPKQGAIAAQKLVDQKVNGVVGHL
                     NSGTTIPASKIYFDAGIPQITPSATNPKYTQQGFKTAFRLLANDNALGAATANYATKT
                     LKAKKVAVVDDRTAYGQGVADIFEKVAKQNGAEVVTRQFTNDKATDFNAILTAIKDKK
                     PEVIFYGGMDSQAGPMLRQLKQLGISAKFMGGDGICTVEIAKLAGDAIGDHVYCAEGG
                     ASLDKMPKGKEFKQRFEAKFKDQIQVYAPYVYDAVMTLAAAMQKANSVEPAKYLPELA
                     KIQYDGVTTKVRFDATGELVDAATTLYSYPGGKKTPLN"
     gene            complement(8642..9103)
                     /locus_tag="AC16HB_06985"
     CDS             complement(8642..9103)
                     /locus_tag="AC16HB_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR5606572.1"
                     /translation="MIAVGVVGTVLPALPGVVFVFGGIVLAAWIDGFTRISGWMLGVL
                     AVLAALGLLVDLVVPAVSARRVGASRSGIVGAAIGTVAGIFTGLVGLIFMPLAGAVIG
                     ELVARRDALRAGQVGLATWLGLLAAAVLKIALVFVMVGIFVAALLFSFRGD"
     gene            complement(9142..10527)
                     /locus_tag="AC16HB_06990"
     CDS             complement(9142..10527)
                     /locus_tag="AC16HB_06990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5606573.1"
                     /translation="MNSPIADPQLVSVRTPPHSIEAEQSVLGGLLLDNAAWDRVADMV
                     RAEDFYRHDHRLIFQYVSRLIDQSRPADAVTVYESLQTAGKAEEAGGLAYLSALAQNT
                     PSAANIRRYGEIVRDRAVLRRLVTVGDEIATSALNPQGKDTKTVLDEAESKVFQIAEE
                     GARGRQGFVEIEPLLTKVVERIQELFERANPSDVTGVPTGFTDVDRMTSGLQPGDLVI
                     VAGRPSMGKTAFALNIGEHVAIDQGLPVAVFSMEMGAAQLALRMLSSVGRINQQRLRT
                     GRLEDGDWPKLTAAIQKMHEAQLFIDETPALNALELRARARRLARTCGKLGLIVIDYL
                     QLMSASSMGENRATEISEISRSLKALAKELQVPVVALSQLNRTVESRTDKRPVMSDLR
                     ESGAIEQDADVIFALYREEFYNPDTPDKGVAEVLILKQRNGPTGRLSLRFAGENTRFD
                     NLAQGFGAAAY"
     gene            complement(10600..11052)
                     /gene="rplI"
                     /locus_tag="AC16HB_06995"
     CDS             complement(10600..11052)
                     /gene="rplI"
                     /locus_tag="AC16HB_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR5606574.1"
                     /translation="MQVILLEKVINLGGLGDVVKVKEGYARNYLIPQGKARRATEAAI
                     KEFEGRRAELEGAQAEKLAAAQAEGEKLNGQKIQLSAKAGVDGRLFGSVTNFDIAEAL
                     TKQGFHVAKGQIRMPTGPLKTIGEHHLTVALHSDVLAEITVTVAGETQ"
     gene            complement(11067..11357)
                     /gene="rpsR"
                     /locus_tag="AC16HB_07000"
     CDS             complement(11067..11357)
                     /gene="rpsR"
                     /locus_tag="AC16HB_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5606575.1"
                     /translation="MAFGPRGKGKGKGKPGGRRDQQKNALFKRKKFCRFTVEKVEQID
                     YKDVETLRDFILDNGKIMPARLTGTKAHYQRQLDTAIKRARFLALLPYSDNH"
     gene            complement(11359..11658)
                     /gene="priB"
                     /locus_tag="AC16HB_07005"
     CDS             complement(11359..11658)
                     /gene="priB"
                     /locus_tag="AC16HB_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HIR5606576.1"
                     /translation="MNRVQLSANLVARAELRYTPAGVAVLEMRIAHQGTVAESGAERS
                     LQFELDAIALGDAAQRLARIDLGAQLNLAGFLAPRSRRSQRLVLHINEFVLIQGV"
     gene            complement(11666..12031)
                     /gene="rpsF"
                     /locus_tag="AC16HB_07010"
     CDS             complement(11666..12031)
                     /gene="rpsF"
                     /locus_tag="AC16HB_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5606577.1"
                     /translation="MRHYEICFIVHPDQSEQVPAMIERYKALVNGQGGKVHRVEDWGR
                     RQLAYPIDKLVKAHYVLMNIECGKATLDELEHGFRYNDAVLRHLTVGTKRAETAPSPM
                     MKVVEREEAKKAASEQVAA"
BASE COUNT         2036 a         4080 c         3896 g         2196 t
ORIGIN      
        1 cgagcgtggc cgcatgccgc ggcgtctcgg tcagcagcgc ccagcgcagt ccggtcgcga
       61 tcagcacctg gcccgcgccg gcctcgcgat agcgatacga gtccttgccc ggccgatcca
      121 tgtcgaagcc gtggtgcgcg ttcttgatcg cagaaaccgc cagcccgcgc gcacggaaga
      181 tcgggatcat cttctcgatc aacgtggtct tgccggtgtt cgagtagccg atgatgccga
      241 tggctttcat ctcttcttgt tgctccagcc ttcgtcgctg ccgcgatcgc gccgatagta
      301 gcgttcgctc gatccgcgcg gaatcgtgct gaaccaatgg acgaaccgcg cggactcgta
      361 gcacttcgtc gccgggacgt tgtcctcgtg cgcgcccgcc accgcgtacg ccgcatcgcc
      421 ccgtgcccac aaccattcgc gcacgaacgc atcgagcagt gccgcaccga gcccgcggcc
      481 gatcaacgcg ggctcgccga tgaaccagtc gagtccccag ccgccacgcg gcagcacgaa
      541 gcccggccca tactgcgcat agtcctcgat ccggtaggtc tgcacgaacc cggccgggcg
      601 tgcgtcgagc agcgcgatca tgcacacgat cggctgctcg ccgcgcacgc gtggcaggta
      661 cttggcgcgc acttcgtcgc gcgtgaagcg gcgccggctc caccagcgat tgacgtgcgg
      721 ctggttcagc cagcccagct gccgcccgag atcgagctcc agcatgggac ggaaccgcca
      781 cttactgcgc atgcgcgacg atgtagtcct tcacgcgcag cgcgtcgtcc ggcagcacga
      841 cgcagcgctg cgggcgctgc tcgatcccct cgtacgctgc gggacgaccc ggcgcgcggc
      901 cgatcgcctc tacgatcgtc tctgcgaatt tcacgggcaa cgcggtctcc aggcaaaccg
      961 tcggcatgcg cgcgtcgaga tgctcgcgcg cgaccttgat gccgtccgcg gtgtgcggat
     1021 cgacgatcgt gccggtggat tcgtacacct ctcggatcac gcggagccga tcggcgtgcg
     1081 tgctcgagcc ggagacgaag ccggacgcgc gcaccttgtc ccagaacggc gtggccgcga
     1141 gatcgaactc gccgcgtgtg tcgacctgcc accacagttc gcgcaccgca tcgggatcgc
     1201 ggccgacgac atcgaacaca tagcgctcga agttcgaagc cttgctgatg tccatcgacg
     1261 ggctgctggt ggcatgcgtt tcccggctcg accgcacccg gtagcgtccg gtgcggaaga
     1321 attcgtcgag cacgttgttc tcgttggtcg cgaggatcag ccgctcgacc ggcagcccca
     1381 tctgcttcgc gatccagccg gccaggatgt tgccgaagtt gcccgacggc acgcagaacg
     1441 acacgcgctc gtcctcgttc tgcgtcgccg cgaaccagcc gcggaagtag tagacgacct
     1501 gtgcgaccac gcgcgcccag ttgatcgagt tgacgctgcc gatccggtag cggcgcttga
     1561 agtcgagatc ggccgatacc ttcttgacga tgtcctggca atcgtcgaac acgccctcga
     1621 tcgcgatgtt gaagatattc gggtcctgca gcgaatacat ctgcgcgcgc tgaaacgcgc
     1681 tcatccgccc gtgcggcgac agcatgaaga cgttgatgcc ggcacggccg cgcatcgcgt
     1741 actcggccgc agaaccggtg tcgcccgaag tcgcgccgag cacattcaac cgctcgccgc
     1801 gttgggcgag tgcgtattcg aacagcgccc cgagcaactg catcgccatg tccttgaacg
     1861 cgagcgtcgg cccgttcgac aggtgcagca ggtgcagccc cggcgcgact ttagtcaacg
     1921 gcgtgatcgc atcggtaccg taggtcgcgc gggtgtaggt cgcgcgcgtc agacggtcga
     1981 ggtcggcgcg cggaatgtcg gtgcagtaat gcgagaggac ctcggccgcc agcgccgcgt
     2041 agtcgagcgc gcgccagcgc gcgagcgtgg cgcggtcgat gcgcggatag aactcgggca
     2101 ccacgaggcc gccgtcgggc gcgagccctt ccagcagaat gtcggtgaac ccggcgggcg
     2161 gcatcttgcc gcgcgtggag acgtagcgca tctagatcag ctcttcctga cggatgcgga
     2221 tgatcgaggc gagcacggtc ggcaacgcct cgatgcgcct gatcgccgcg ttcacgtcgc
     2281 gttcgacggc atcgtgcgtc agcatgatga tgtcggtctg gttctcgccc tcgctcggct
     2341 cgcgctggaa cagcgcgtcg atcgagatcc tgcggtcggc gaggatgcgc gtgatgtccg
     2401 ccagcacgcc gggctggtcg gccacccgca tgcggaagta atacgaggtc accgtgtcgt
     2461 cgatcgacag ccatggcacc tcgaccatcg cctcggcctg gaatgcgaga tgcggcacgt
     2521 ggttctccgg gtcggcggtg tgcatgcgcg tgacgtcgac cagatccgcg accacggcgg
     2581 aggcggtcgg ctctgcgccc gcccccttgc cgtagtacaa ggtggtgccg accgcgtcgc
     2641 ccttgaccag caccgcgttc atcgcgcctt cgacattggc cagcagccgc tgcatcggca
     2701 ccagcgtcgg gtgtacccgc aattcgatgc catggtcggt gcgcttggta atgccgagca
     2761 gcttgatccg gtatccgagc tgttccgcat agcggatgtc cgcggctgcg agcttcgaga
     2821 taccctcggt ataggcggcg tcgaagttga tcggcacacc gaaggcgatc gacgacagga
     2881 tggtcagctt gtgcgcggca tcgatccctt cgatgtcgaa ggtcggatcg gcttccgcgt
     2941 aaccgagccg ctgtgcttcc ttcagcaccg cgtcgaacgg cagccccttc gaccgcatct
     3001 ccgacaggat gaagttgctg gtgccgttga tgatcccggc aatccattcg atgcgattgg
     3061 ccgtcaggcc ctcgcgcagc gccttgatga tcgggatgcc gccggcgacc gcagcttcga
     3121 acgcaaccat cacgccctgc gcgcgcgcgg cctcgaagat ctcgttgccg tgcctggcca
     3181 gaagcgcctt gttggcggtc accacatgct tgccacgcgc gatcgcgtcc agcacgtagg
     3241 tacgcgctgg ctcgtagccg ccgatcaact ccacgacgat gtcgatgtcg tcgcggcgga
     3301 tgacttcggc cgcgttggcg gtgatcggta cggtcgtgcc aattgcgtcg tgtgccttgt
     3361 gcacgttacg taccgcgatc gcggcgatct cgatgccgcg cccggcgcgc cgcgtgattt
     3421 cgtgctgatt gcggcgcagg acgttgaaca ccccgcgccc gaccgtgccc aggcccagca
     3481 aaccgacttt gattggcttc atctcgaccg tcccgcgtcc gcgcgggcgc attcgtggtt
     3541 aaccgcgttc gcgggtgcgt tcaggccgcc gccctcggca gcagcccgtc gcgtcggaac
     3601 atttccttga tgccacggac cgcctgtctg attcggtgct cgttttctat cagcgcgaac
     3661 cgcacgtgat cgtcaccgta gtcgccgaag ccaatacccg gcgatacggc gacctttgcc
     3721 tcggacagta ccttcttcgc gaattcgagc gagcccatcg gtttgtaagg aggcgggatc
     3781 tcggcccaga tgtacatcga tgccttcggc acttcgacca tccagcccgc ttcctgcagg
     3841 cccttggcga gcacatcgcg gcgccgctga tactgagcgc ggacctcctc gacgcagtcc
     3901 tggggtcctt ccagcgcggc gatcgacgcg acctggatcg gggtgaacgt gccgtagtcg
     3961 tgatagctct tgatccgcgc cagcgcgctg accaggtcct tgttgccgac catgaagccg
     4021 atgcgccagc cggccatgtt gtagctcttg ctcatcgtga agaactcgac cgcaacgtcg
     4081 cgcgcgcccg gcacttccat gatcgacggc gcccggaagc cgtcgaagac gatgtcggcg
     4141 tatgcgaggt cgtgcacgac gtagatgccg tgctgcttcg ccagcgcgac cacgtgatgg
     4201 aagaagtcca gatcgacgca ctgcgcggtc gggttgctcg gaaatccgag gatcatcatc
     4261 ttcggcttcg ggaacgactc gcggatcgcg cgctccagtt ccgcgaagaa atccacttcg
     4321 cgcgtcatgc ggaccgagcg gatgtcggcg ccggcgatga tcgcgccgta gatgtgaatc
     4381 gggtagctcg ggttcggcac cagcacggtg tcgccacggt cgagcgtggc aagcatcagg
     4441 tgcgccagcc cctccttcga gccgatcgtc acgatcgcct cggagtctgg atcgaagtcg
     4501 actgcgaacc gcgtcttgta ccagtgggtg atcgagcgcc gcagccgcgg aatgcccttg
     4561 gacaccgagt agccgtgcgt gtcagggcgc tgcgccgcct cgaccagctt gtcgacgatg
     4621 tgcttcggcg tgggtccgtc cggattgccc atgctcatgt cgatgatgtc ttcgccgcgc
     4681 cgtcgcgcgg ccatcttcaa ctcgccggtg atgttgaaga cgtagggtgg caggcgcttg
     4741 atgcgcggga attcgaccat ccaggttgcc tcgagcctat gagcgcttgt gcaatcagcg
     4801 ccattgggca taatttaccg tgaatcgcag ggtgctgctg ctgcgcagca agcttcgctc
     4861 aatcgcgcgg cagcccgaac cggagtcgaa ttgaagcttc acgcagacgc gctgaactcg
     4921 ctcaacaccg tcaccgccta cggacccggc ttcgtcgaaa tcaaccaggt gcgccatcga
     4981 ggcaacctga tcctcgtccc cgagcaagca gtggcagcct ggagcgccgt cagcttcgaa
     5041 gcgctgcgcg tcgaggattt cgacgcgctg ctcgaactgc gccctgaagt ggtgctgctc
     5101 ggcaccgggg cgcggcagcg ctttccgcat ccgcgtctga cgcgcaacct ggccggagcg
     5161 cacatcggcg tcgaagcgat ggacacgcgg gccgcctgcc gtacgtacaa catcctgatg
     5221 aacgaaggac gccgcgtcgt cgcggtgttc ctgcaggacg actgagagga ccgatggccg
     5281 tcgcactcga caagaaggtt cccgacttcg gcgcggcctc caccgcgggc gagttcaagc
     5341 tgtccgcgct gaagggcagg tcggtggtgc tgtacttcta tccgaaggac aacacgcccg
     5401 gatgcacgac cgaaggctcc gacttcagcg tcgcgtacgc gaggttcagg aaggccggtg
     5461 cggaagtgct cggcgtgtcg cgcgacagcc tgaagtcgca cgaaaacttc aaggcgaaga
     5521 tgaagttccc gttcgagctt ctgtccgacc ctgacgagaa gctgtgcaca ctgttcggcg
     5581 tgatcaagat gaagaacatg tacggcaaac aggtgcgcgg gatcgagcgc agtaccttcg
     5641 tgatcgatgg cgagggcagg ctcaggcgcg agtggcgcgg cgtgaaggtg ccgggccatg
     5701 tcgacgaggt gctggccttc gttcagtcgc tgtaaccctt tccaacctca cggccaagcg
     5761 ccgtacgctg atgcccctgc cgaagccgcc gagcaagccc gccaccctac tgacatcgat
     5821 tcccaccgcc gcagcgacgc cgcgcaagga cacggcgaac gtccggccgc aagccacaga
     5881 aatccactct gcaccccagg cgcgcgcgcc gtcgtcgccg gctgccgagc agcgcgcctc
     5941 ggccgccgcg caccttgcgc tcgtcgaggc caggccgaag aacggcaaga agccccgcgt
     6001 cagtggcgcc gcccggcgcc tgttcgtgct cgacaccaac gtgctaatgc acgacccgac
     6061 gtcgctgttc cgcttcgagg agcacgacgt tttcctcccg atgatgacgt tggaggaact
     6121 cgacagccac aagaagggca tgtccgaggt tgcgcgcaac gcgcgccagg tcagccgctc
     6181 gctcgattcg ctggtcgccg cgtgcaagac gccgatcgag gacggcatcc cgctggacgc
     6241 gctcggcaat gtcgatgcgc gcggccgctt gttcttccag acgcaggcag cggcaggccc
     6301 gctgccggcc acgctgccgg ccggaaaggc cgacaaccag atcctcggcg tagtcaaggc
     6361 cctgcaggac acccatcccg gccgcgacgt ggtgctggtg tcgaaggaca tcaacatgcg
     6421 cgtgaaggcg cgcgcgctcg gattgccggc cgaggactac tacaacgaca aggtgctgga
     6481 ggacaccgac atcctctaca ccggcgtgct cgcactaccg gccaacttct gggacaagaa
     6541 cggcaagggc atggagtcgt ggcagcagag cggctacacg ttctatcgca tcaccggccc
     6601 tatcgccgac agcctgctcg tcaaccagtt cgtctatctg gaggacgggg cgccattcca
     6661 cgcgcaggtg cgcgagatcc gcagcaaaac cgccgtcctg cgcgtactgc gcgactacca
     6721 gcatgcgaag aacgccgtgt ggggcatcac cgcccgcaat cgcgagcaga gctttgcgct
     6781 gaacctgctg atggatccgg agtgcgactt cgtgacgctg atcgggcagg cgggcaccgg
     6841 caagacgctg ctcgcgctcg ccgccgggct ggcgcagacg ctggagacca agcgcttctc
     6901 ggagatcatc gtcacgcgcg ccaccgttcc ggtcggcgag gacatcggct tcctgcccgg
     6961 caccgaagaa gagaagatgg cgccgtggat gggcgcgctg gaggataacc tcgaggtgct
     7021 gaacaagtcc gacgactcgg ccggcgaatg ggggcgcgcc gcgaccaacg atctgatccg
     7081 cacgcgcatc cgcatcaagt cgctgaattt catgcgcggc cgcccgttca tccacaagta
     7141 cctgatcatc gacgagtgcc agaatctgac gccgaagcag atgaagacgc tgatcacgcg
     7201 cgccgggccc ggtaccaagg tgatctgcct cggcaacatc gcgcagatcg acacgccgta
     7261 cctgaccgag ggcagttcgg gcctgacttt cgtggtcgac cgtttcaagg gctggccgca
     7321 ttcgggccac gtgacgctgc agcggggaga gcgctcgcgg ctcgccgatc acgcggccga
     7381 ggtgctgtga cgcgggcacc aaagaaaacg cccggcgaac cgggcgtttg cgatcgagcg
     7441 taccgggatc agttgagcgg ggtcttcttg ccgcccgggt agctgtacag cgtggtcgcc
     7501 gcatcgacca gttcgccggt ggcatcgaag cgaaccttgg tcgtcacgcc gtcgtactgg
     7561 atcttggcca gctcgggcag gtacttggcc ggctcgaccg agttggcctt ctgcattgcg
     7621 gccgccagcg tcatcactgc gtcgtacacg tatggcgcgt agacctggat ctggtccttg
     7681 aacttggctt cgaaacgctg cttgaattcc ttgcccttcg gcatcttgtc cagcgacgcg
     7741 ccgccctcgg cgcaatacac gtgatcgccg atcgcgtcac ccgcgagctt ggcgatctcg
     7801 accgtgcaga tgccgtcgcc gcccatgaac ttggcgctga tgccgagctg cttcagctgt
     7861 cgcagcatcg gtccagcctg cgagtccatg ccgccgtaga agatcacctc gggcttcttg
     7921 tccttgatcg cggtcagaat ggcattgaag tcggtggcct tgtcgttggt gaactggcgc
     7981 gtgacgacct cggcaccgtt ctgcttggcg accttctcga agatgtcggc cacgccctgg
     8041 ccgtacgcgg tgcggtcgtc gaccactgcg accttcttcg ccttcagcgt cttggtcgcg
     8101 taattggcgg tcgcggcgcc caatgcgttg tcgttggcca gcaggcggaa cgcggtcttg
     8161 aagccctgct gcgtgtactt cgggttggtt gccgatggcg tgatctgcgg aatgccggcg
     8221 tcaaaataga tcttcgaagc cggaatcgtc gtgcctgagt tcaggtgacc caccacaccg
     8281 ttgaccttct ggtcgaccag cttctgcgcc gcgatggcgc cctgcttcgg gtccgcggcg
     8341 tcgtcctcgc tgatcagctt ccatttgatc ttcttgccgc cgatcgtgag gttctgcgca
     8401 ttcaggtcgt cgatcgccat gatggcgccg cgctcgttgt ccttgccgta atgcgcctgg
     8461 ccgccggaca gcggtgcgac atgaccgatc ttgacttcga cctcctgtga ccaggcggtg
     8521 cccgcgaaca cgaggccaac cgccactccc agtagtgctt tcttcatcct ccgtctcctt
     8581 tctgaagtcc ggaagccata cctccggaag cgcaggaatg tagcacttgc gggccgaccg
     8641 ctcagtcccc gcgaaaagaa aagagcagag cggcgacgaa gatcccgacc atgacgaaaa
     8701 cgagggcgat cttcagaacc gcggcggcca gcaggccgag ccaggtcgcc agccccacct
     8761 gcccggcgcg cagcgcgtcg cggcgcgcga ccagttcgcc gatgaccgcg ccggcgagcg
     8821 gcatgaagat caagccgacg aggccggtga agatgccggc aaccgtgccg atcgccgcgc
     8881 cgacgatgcc cgaccggctc gcgccgaccc ggcgggcgga cacggccggc accacgaggt
     8941 cgaccaagag tcccaacgca gcgagcactg cgagcacccc gagcatccag cccgagatgc
     9001 gggtgaaacc gtcgatccag gcggcgagca cgatgccgcc gaagacgaag accacacccg
     9061 gcagcgccgg cagtacggtg ccaacaacgc cgaccgcgat catcgcgatc gcaacgatcc
     9121 acagcgcggt cgtcatgcgc ttcagtacgc ggcggcgccg aagccctgcg cgaggttgtc
     9181 gaagcgcgtg ttctcgccgg cgaagcgcag gctcaggcgt ccggtcgggc cgttgcgctg
     9241 cttcaggatc agcacctcgg ccacgccctt gtcgggtgtg tcggggttgt agaactcctc
     9301 ccgatacagg gcgaagatca cgtcggcatc ctgctcgatc gcacccgact cacgcaggtc
     9361 gctcatcacc gggcgcttgt cggtacggct ctcgaccgtg cggttgagct gcgacagagc
     9421 caccaccggc acctgcagtt ccttggcgag tgccttcagt gagcgggata tttcggagat
     9481 ctcggtggcg cggttctcgc ccatgctgga cgccgacatc agctgcaggt agtcgatcac
     9541 gatcagccca agcttgccgc aggtgcgcgc cagccgccgc gcgcgcgcgc gcagctccag
     9601 cgcattgagt gcgggggtct cgtcgatgaa gagctgtgcc tcgtgcatct tctggatcgc
     9661 cgcggtgagt ttcggccagt cgccgtcttc gagccgcccg gtgcgcaacc gctgctggtt
     9721 gatgcggcca accgaactga gcatgcgcaa agcaagctgc gcggcaccca tctccatcga
     9781 gaacaccgcg acgggcagcc cttgatcgat cgcaacgtgc tcgccgatgt tgagcgcgaa
     9841 cgccgtcttg cccatcgacg gccgcccggc gacgatgacc agatcacccg gctgcaagcc
     9901 cgaggtcatg cggtcgacgt cggtgaagcc cgtcggcacc ccggtcacgt cggatgggtt
     9961 cgcgcgctcg aagagttcct ggatccgttc gaccaccttg gtgaggagcg gctcgatctc
    10021 gacgaagccc tgccggccgc gcgcgccctc ctccgcgatc tggaacacct tagactcggc
    10081 ctcatccagc actgtcttcg tgtccttgcc ctgcggattc agcgccgacg tggcgatctc
    10141 gtcgccgacc gtcaccagcc gccgcagcac cgcgcgatcg cgcacgatct cgccgtagcg
    10201 acggatattg gccgccgacg gcgtgttctg cgcaagtgca ctgaggtacg cgagtccgcc
    10261 cgcctcctct gccttgccgg cggtctgcag cgactcgtaa accgtgaccg cgtcggcggg
    10321 ccgcgactgg tcgatcagcc tgctgacgta ctggaagatc agccggtggt catggcggta
    10381 gaaatcctcc gcccgcacca tgtcggcaac gcgatcccat gcggcgttgt cgagcagcag
    10441 tccgccgagc acggattgct cggcctcgat cgagtgcggc ggcgtgcgaa ccgaaacgag
    10501 ctgcgggtcc gcgatcggag aattcatgga gtccggcgtt gaaaagttca gcgtccgggc
    10561 gcatgtcccg cccgacgctc aacgcggaac attaccggat tactgcgtct cgcctgcgac
    10621 cgtcactgtg atttcggcca gcacgtcgct atgcagcgcc accgtcaggt gatgctcgcc
    10681 gatcgtcttc agcgggccgg tgggcatgcg gatctggccc ttcgcgacgt ggaagccctg
    10741 cttggtcagc gcctcggcga tgtcgaagtt cgtgaccgag ccgaacaggc gcccatcgac
    10801 gccggccttg gcgctgagct ggatcttctg gccgttgagc ttctcacctt cggcctgtgc
    10861 ggccgctagc ttttcggcct gtgccccttc gagctcggcg cggcggcctt cgaattcctt
    10921 aatcgccgcc tcggtggcgc ggcgcgcctt gccctgcggg atcaggtagt tgcgcgcata
    10981 gccttctttg accttgacta cgtcgccgag gccgccgagg ttgatcactt tttcgagcag
    11041 gatcacttgc atcgtgttct ccgcgctcag tggttgtccg agtacggcag cagcgcgaga
    11101 aagcgtgcgc gcttgatcgc cgtgtcgagc tggcgctggt agtgcgcctt ggtgcccgtc
    11161 aggcgtgccg gcatgatctt gccgttgtcg agtatgaagt cgcgcagcgt ctccacgtcc
    11221 ttgtagtcga tctgctcgac cttttcgacc gtgaagcggc agaacttctt gcgcttgaac
    11281 agcgcgttct tttgctggtc gcgtcggccg cccggcttac ccttgccctt gcccttgccc
    11341 ctcggtccga atgccatttc aaactccttg aatcagtacg aattcgttga tgtgaagaac
    11401 caatcgctgt gaccgccggc tccgcggcgc aaggaatccg gcgagattca gttgcgcgcc
    11461 cagatcgatc cgtgccaacc gctgcgccgc gtcgccgagc gcaatcgcgt cgagttcgaa
    11521 ctgcagcgat cgctccgccc cagactctgc caccgtgccc tgatgagcga tgcgcatttc
    11581 caggaccgca acgcccgccg gcgtgtagcg cagttcggcg cgggcgacga ggttcgcgct
    11641 gagctgaacc cgattcaatc gtgcactagg cagccacctg ttcgctcgcc gccttcttgg
    11701 cctcttcgcg ctcgaccacc ttcatcatcg gcgacggcgc cgtttccgcg cgcttggtac
    11761 cgacggtcag gtgccgcagc accgcgtcgt tgtagcggaa gccgtgctcg agctcgtcga
    11821 gcgtggcctt gccgcactcg atgttcatca ggacgtaatg cgccttgacg agtttgtcga
    11881 tcgggtacgc aagctggcgg cgaccccagt cctcgactcg atgaaccttg ccgccctggc
    11941 cgttgaccag cgctttgtag cgctcgatca tcgccggcac ctgctcgctc tggtccggat
    12001 ggacgatgaa gcagatttcg tagtgacgca ttcgatctcc ttgtggttta agccaccccg
    12061 agcgtcgttc gagtgtggca aggctgcgaa gagccgtgca ttttacctgc aaacgcaagc
    12121 ccggagcaaa gcgggccggc gccggaattc agtcggccgc cagtatccgc ggcagcgtct
    12181 cgccggcgcg cgcgcggacg cagacctc
//
LOCUS       DBNHBY010000284         3159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_422503, whole genome shotgun sequence.
ACCESSION   DBNHBY010000284 DBNHBY010000000
VERSION     DBNHBY010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_422503"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="atpA"
                     /locus_tag="AC16HB_07015"
     CDS             <1..1277
                     /gene="atpA"
                     /locus_tag="AC16HB_07015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5606578.1"
                     /translation="STGRILEVPVGPELIGRVVNSLGQPIDGKGPINAKMTDVIEKVA
                     PGVIARKSVGQPVQTGLKAIDSMVPIGRGQRELIIGDRQTGKTAVAIDTIINSKGKDL
                     FCIYVAIGQKASTIANVVRKLEETGAMAYTIVVAASASESAAMQYIAPYSGCTMGEYF
                     RDRGQDALIIYDDLTKQAWAYRQVSLLLRRPPGREAYPGDVFYLHSRLLERAARVNEA
                     YVEKLTKGAVKGRTGSLTALPIIETQAGDVTAFVPTNVISITDGQIFLETDLFNAGIR
                     PAINAGISVSRVGGAAQTKVIKKLGGGVRLALAQYRELAAFAQFASDLDDATRKQLER
                     GRMVTELMKQPQYHPLQVWEQAITLFAVNNGYFDDIDVKRALGAEKALRDYLKSKHGA
                     LVERIESSKDLTKEDEEALHAAIKEFKRSGAY"
     gene            1299..2180
                     /gene="atpG"
                     /locus_tag="AC16HB_07020"
     CDS             1299..2180
                     /gene="atpG"
                     /locus_tag="AC16HB_07020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR5606579.1"
                     /translation="MPSTKEIRLKIKSVQNTRKITKAMEMVAASKMRKAQERMRQARP
                     YGDKIRNIAAHLASANPEYRHPFVVKRRGLRNIGLIMVSTDKGLCGGLNTNIQRALLQ
                     RVKQWEADGVKFQATAIGNKGLGFLQRLNANVVSSVVQLGDKPHMERLIGPIKLQLDA
                     FAEGRLDAVFIAYSRFVNTMKQEPMIEQLLPLSAEMLAQSESEKRAYAWDYLYEPDAQ
                     RVIDELMTRYVETIIYQSVVENMACEQSARMVAMKSASDNAKKLIDELQLIYNKTRQA
                     GITKEISEIVGGAAAVS"
     gene            2318..>3159
                     /locus_tag="AC16HB_07025"
     CDS             2318..>3159
                     /locus_tag="AC16HB_07025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5606580.1"
                     /translation="MSTGTIVQCIGAVVDVEFPREQMPRIYDALKLEIDAGNTLVEQG
                     LTFEVQQQLGDGIVRTIALGSSDGIRRGMKVKNTGDGIKVPVGPATLGRVMDILGRPI
                     DERGPVQADEYRTIHQPAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFGGAGV
                     GKTVNMLELINNIAKQHSGLSVFAGVGERTRDGNDFYHEMSDAKVIVQEDLRQSKVAM
                     VFGQMNEPPGNRLRVGLTGLTMAERFRDEGRDILFFVDNIYRYTLAGTEVSALLGRMP
                     SAVG"
BASE COUNT          646 a          958 c         1069 g          486 t
ORIGIN      
        1 agtcgaccgg ccgcattctc gaagtgccgg tcggtccgga actgatcggc cgtgtcgtga
       61 attcgctcgg ccagccgatc gacggcaagg gaccgatcaa cgccaagatg acggacgtga
      121 tcgagaaggt cgcgccaggc gtgatcgcgc gcaagtcggt gggccagccg gtgcagacgg
      181 gcctgaaggc gatcgactcg atggtgccga tcggccgtgg ccagcgcgag ctgatcatcg
      241 gcgaccgcca gaccggcaag accgcggtgg cgatcgacac gatcatcaac tccaagggca
      301 aggatctgtt ctgcatctac gtcgcgattg gccagaaagc ctcgacgatc gccaacgtgg
      361 tgcgcaagct cgaagaaacc ggcgcgatgg cctacacgat cgtggtggcg gcatcggcgt
      421 ccgaatcggc ggcgatgcag tacatcgcgc cgtactccgg ctgcacgatg ggcgaatatt
      481 tccgcgaccg cggccaggac gcgttgatca tctacgacga cctgaccaag caggcgtggg
      541 cctatcgtca ggtgtcgctg ctgctgcgcc gtccgccggg ccgggaagcg tatcccggcg
      601 acgtgttcta tctgcactcg cgcctgctcg aacgcgccgc gcgcgtcaac gaggcgtacg
      661 tggagaagct caccaaaggt gcagtcaagg gcaggaccgg gtcgctgacc gcgctgccga
      721 tcatcgagac gcaggccggc gacgtgaccg cgttcgtgcc gaccaacgtc atctcgatca
      781 ccgacggcca gatcttcctc gagaccgacc tcttcaacgc cggcatccgc ccggcgatca
      841 acgccggcat ctcggtgtcg cgcgtcggcg gcgcggcaca gaccaaggtg atcaagaagc
      901 tgggcggcgg cgtacgtctg gcactcgcgc agtaccgcga gctggcggcg tttgcgcagt
      961 tcgcgtccga cctcgacgac gcgacgcgca agcagctcga gcgcggccgc atggtcaccg
     1021 aactgatgaa gcagccgcag taccacccgc tgcaggtgtg ggagcaggcg atcacgctgt
     1081 tcgcggtcaa caacggctac ttcgacgaca tcgacgtgaa gcgcgcgctc ggcgcggaga
     1141 aggcgctgcg cgattacctg aagagcaagc acggcgcgct ggtcgagcgc atcgaatcgt
     1201 ccaaggatct gacgaaggag gacgaggagg cattgcacgc cgcgatcaag gagttcaagc
     1261 gctcgggcgc gtactgatcg actgcgccgg gaatcgccat gccgagtacc aaagaaatcc
     1321 gcctgaagat caagagcgtg cagaacacgc gcaagatcac caaggcgatg gagatggtcg
     1381 ccgccagcaa gatgcgcaag gcgcaggagc gcatgcgcca ggcgcgaccc tacggcgaca
     1441 agatccgcaa catcgcggcg catctggcgt cggccaaccc ggagtaccgg cacccgttcg
     1501 tggtcaaacg tcgcggtttg cgcaacatcg ggttgatcat ggtgtcgacc gacaagggcc
     1561 tgtgcggggg gctcaacacc aatatccagc gcgcgttgct gcagcgggtc aagcagtggg
     1621 aagcggatgg cgtgaagttc caggccaccg cgattggcaa caagggcctc gggttcctgc
     1681 agcggctgaa tgcgaatgtc gtgtcgagcg tggtgcagct cggcgacaag ccacacatgg
     1741 aaagactgat cggaccgatc aagctgcaac tcgacgcgtt tgccgagggg cggctggacg
     1801 cagtcttcat cgcgtattcg cgcttcgtca atacgatgaa gcaggagccg atgatcgagc
     1861 agttgctgcc gctgtcggcc gaaatgctcg cgcagagcga gtccgagaag cgcgcctacg
     1921 cgtgggacta cctgtacgag cccgacgcgc agcgcgtgat cgacgagctg atgacccgct
     1981 acgtcgagac gatcatctac cagtcggtgg tcgagaacat ggcgtgcgag cagagcgcgc
     2041 gcatggtggc gatgaaatcg gcttcggaca acgccaagaa gctgatcgat gaacttcagc
     2101 tcatctacaa caagacacgg caggccggga tcaccaaaga gatttcggaa atcgtcggcg
     2161 gcgcggccgc ggtttcctag tgatgagtcg aacgccgcga ttcggcgagg ctaacttgaa
     2221 cgaagtgaaa gttaagagag cgcgagcgag cggccggagc caaatcgtcg gcggcgcggc
     2281 cgcggtgtcc taacgatttc gaaagcaagg aagagtcatg agtaccggaa ccattgttca
     2341 gtgcatcggc gccgtggtgg atgtggagtt cccgcgcgag cagatgccca ggatctacga
     2401 cgcgctgaag ctggagatcg atgccggcaa cacgctggtc gagcaggggc tcactttcga
     2461 ggtgcagcag caactcggcg acggcatcgt gcgcacgatc gcgctcggat cgtcggacgg
     2521 gatccggcgc ggcatgaagg tcaagaacac cggcgacggc atcaaggtgc cggtcggccc
     2581 cgccacgctc ggccgcgtga tggacatcct cggacgcccg atcgacgagc gcggtccggt
     2641 gcaggccgac gagtaccgca cgatccacca gccggcgccg aagttcgacg aactttcgcc
     2701 gtcggtcgag ctgctggaga ccggcatcaa ggtgatcgac ctgatctgcc cgttcgccaa
     2761 gggcggcaag attggcctgt tcggcggcgc cggggtgggc aagacggtga atatgctcga
     2821 gctgatcaac aacatcgcca agcagcactc gggtctgtcc gtgttcgctg gcgtgggcga
     2881 gcgcacgcgc gatggcaacg acttctatca cgagatgtcg gacgccaagg tcatcgtgca
     2941 ggaagacctg cgccagtcga aggtggcgat ggtgttcggc cagatgaacg aaccgccggg
     3001 caaccggctg cgcgtgggcc tgacgggcct gacgatggcc gaacgcttcc gcgacgaagg
     3061 ccgcgacatc ctgttcttcg tcgacaacat ctatcgctac acgctcgccg gcaccgaagt
     3121 gtccgcgctg ctcggccgca tgccgtcggc ggtgggcta
//
LOCUS       DBNHBY010000285         2316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_761868, whole genome shotgun sequence.
ACCESSION   DBNHBY010000285 DBNHBY010000000
VERSION     DBNHBY010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_761868"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="AC16HB_07030"
     CDS             complement(<1..940)
                     /locus_tag="AC16HB_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606581.1"
                     /translation="MKLKLLSAAALAALLGAAAAQDLKVGVTLSATGPAASLGIPERN
                     TIALLPKTIAGHKVDYIVLDDGSDTTTAVKNTRKLITEDKVDVVVGSTVTPNSLAMID
                     VAAENETPMVSMAASARIVDPVDAKRRWVFKTPQNDQQMATVIVGHMLGNGIKTVAYI
                     GFADAYGEGWWGAFSTIAEGRGLKVVGNERFQRTDTSVTGQVLKIMSLKPDAVLIGGS
                     GTPAALPQKTLKERGYAGVIYQTHGVANNDFLRVCGKDCEGTYLPAGPVLVADQLPND
                     HPVKKSALAYIAAYEKAQGKGSVSTFGAHAWDASILL"
     gene            complement(1017..1727)
                     /locus_tag="AC16HB_07035"
     CDS             complement(1017..1727)
                     /locus_tag="AC16HB_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5606582.1"
                     /translation="MARSRAATFDAQRDAMLAAAARLFAERGYPVASVAGLAQALGVS
                     KALLYHYYRDKEQMLFDIVDRYLDGLIAAVDEVKARQLPADEHLRAMIERLMRAYEHS
                     AAYHRVLVQDVKYLSPPHRNRARAKQRRVVDAFADSIVAVAPELAKRALAKPVTMVLF
                     GMMNWTFTWLRENGALTYADLAPVVAGIFLGGVRQIKEAWPAKAKNGAHSAGQLRDNH
                     DKSRRPASRARLAETSGV"
     gene            complement(1727..>2316)
                     /locus_tag="AC16HB_07040"
     CDS             complement(1727..>2316)
                     /locus_tag="AC16HB_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5606583.1"
                     /translation="EALEDLKDRYLTRFALYNVFSREEQEVGLFNGRIDAAKTRAFLD
                     TLVPADSIDAAFICGPASMIDEVESALLAADVPPKRVHVERFGTPGTEAAPAPVADAA
                     EARVTLVIDGLKREVDFRREHGSILDAGVAAGLDLPFSCKGGMCCTCRAKLIEGEVKM
                     RKNFALEPTDVDAGFVLTCQSYPLTGRVVLSYDDR"
BASE COUNT          361 a          811 c          738 g          406 t
ORIGIN      
        1 gcagcaggat gctggcgtcc cacgcgtgcg cgccgaacgt cgacacgctg cccttgccct
       61 gcgccttctc gtacgcggcg atgtacgcca gcgccgactt cttgaccgga tgatcgttcg
      121 gcaactggtc ggcgaccagc accgggcccg cgggcaggta cgtgccctcg caatccttgc
      181 cgcacacgcg caggaagtcg ttgttggcca cgccgtgcgt ctggtagatc acgcccgcgt
      241 agccgcgctc cttcagcgtc ttctgcggca aagcggccgg cgtgccggat cccccgatca
      301 gcaccgcatc gggcttcaac gacatgatct tcaggacctg ccccgttacc gacgtgtcgg
      361 tgcgctggaa gcgttcgttg ccgacgacct tcaggccacg gccttcggcg atcgtcgaga
      421 atgcgcccca ccagccctcc ccgtacgcgt cggcgaagcc gatgtaggcc acggtcttga
      481 tcccgttacc cagcatgtgg ccgacgatca cggtagccat ctgctggtcg ttctgcggcg
      541 tcttgaacac ccagcgccgc ttggcgtcga ccggatcgac gatgcgcgcc gacgcggcca
      601 tcgagaccat cggcgtctcg ttctcggccg ccacatcgat catcgccagc gagttcggcg
      661 tcaccgtcga cccgaccacc acgtcgacct tgtcctcggt gatcagcttg cgcgtgttct
      721 tcaccgcggt cgtggtgtcg gaaccgtcgt cgaggacgat gtagtcgacc ttgtgaccgg
      781 cgatcgtctt cggcaacagc gcgatcgtgt tgcgctcggg aatgccgagc gacgcggccg
      841 ggccggtggc cgacagcgtg acgccgacct tgaggtcctg agcggcggcg gcgcccagga
      901 gggccgccaa cgcggcggca gaaagcagtt tcagtttcat cttcactggt ctcctctagc
      961 gagtcttctg gatcggtctt tgcggccggg cgccgacgac tacccctgaa acttaatcaa
     1021 actccacttg tttcggcaag gcgtgcgcgg cttgcgggcc ggcgcgactt gtcgtggtta
     1081 tcccgtagct gtccggcgga gtgcgcgcca ttcttcgctt tcgccggcca cgcctccttg
     1141 atctgcctca cccccccgag aaagattccc gccacgaccg gcgccagatc cgcgtaggtg
     1201 agcgcgccgt tctcgcgcag ccacgtgaaa gtccagttca tcatgccgaa cagcaccatg
     1261 gtcaccggct tggcgagtgc gcgtttcgcg agttccggcg ccaccgcgac gatcgaatcg
     1321 gcgaacgcgt cgaccacgcg ccgctgcttg gcgcgtgcgc gattgcgatg cggcgggctc
     1381 agatatttca cgtcctgcac cagtacacgg tggtacgcgg ccgagtgctc gtaggcgcgc
     1441 atcagccgtt cgatcatggc gcgcaggtgc tcgtcggccg gcaactgccg ggccttcact
     1501 tcgtccaccg ccgcaatgag gccgtccagg taacggtcga cgatgtcgaa cagcatctgc
     1561 tccttgtcgc ggtagtagtg gtagagcagc gccttggaca cgcccagcgc ctgtgccagg
     1621 ccggccaccg atgcgacggg gtagccgcgc tccgcgaaca ggcgcgccgc cgccgcgagc
     1681 atcgcgtcgc gctgcgcgtc gaaggtggcg gcgcgcgatc tcgccatcag cgatcgtcgt
     1741 aggagagcac gaccctcccg gtcagcgggt atgactggca ggtcagcacg aatcccgcat
     1801 cgacgtcggt gggctcgagc gcgaagttct tgcgcatctt cacctcgccc tcgatcagct
     1861 tggcgcggca ggtgcagcac atgccgccct tgcacgaaaa cggcaggtcg agcccggcgg
     1921 ccacgccggc gtcgaggatc gaaccatgct cgcgccggaa atcgacctcg cgcttcagac
     1981 catcgatcac cagcgtcacg cgagcttcgg cggcgtcggc gaccggcgcg ggcgccgcct
     2041 ccgtgcccgg cgttccgaag cgctccacgt gcacacgctt gggcggcacg tcggcggcga
     2101 gcaacgcgga ttcgacctcg tcgatcatcg atgccggacc gcagatgaac gccgcatcga
     2161 tcgagtcggc cggcaccagc gtgtcgagaa acgcgcgcgt cttggccgcg tcgatgcgcc
     2221 cgttgaacag acccacctcc tgctcctcgc gcgagaacac gttgtacagc gcgaagcggg
     2281 tcaggtagcg atccttcagg tcctccagcg cctcgt
//
LOCUS       DBNHBY010000286         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1505991, whole genome shotgun sequence.
ACCESSION   DBNHBY010000286 DBNHBY010000000
VERSION     DBNHBY010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1505991"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="AC16HB_07045"
     CDS             <1..1673
                     /locus_tag="AC16HB_07045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5606584.1"
                     /translation="PVDDRLLRAADAETQWQREFTEQIGTDREVLNRSGIGIKALYTN
                     RDWDSSRHANDLGYPGQLPYTRGIYPTMHRGRTWTQRQLIGLGTPDDYNARLRAVLAQ
                     GGNAVSLIPCNSVFRGVDMDESHFELLGTCGTVVNSVEHMDRALAGVDLARTSCAMND
                     PSPFTLLAFMLAVAKRRGVDWRAITGTSNQSDYLSHYVANHMFFRIALPGARRILGDH
                     IDFCNRFVPRWNPMSVVGQHMQQAGATPAEAMAFTLATALQVAQDCIDRGMAPDSFLP
                     RFTFFFDISLSFFEEVAKFRAGRRIWARLARERLGAQEPRSWRFKFHGQTSGVDLTRQ
                     QPLNNIARVTVQAMAGIFGGLQSLHTDAFDEALSTPTDFGARIAVNTQNILREEAHLT
                     DVIDPLGGSFYVEKLTDQMEEEILRVIRIVDDAGGMYRAVEAGLVQKMIGESARRFQQ
                     QVESGAQTVVGVNAYTAAEDGSARQPLARPDAATMRAHLEAFRAYKALRSQAVVDSAL
                     SALARAANDPRDNVFAQVIAAAEAGCTHGEICGTLRREFGFGHVQAFV"
     gene            1676..>2069
                     /locus_tag="AC16HB_07050"
     CDS             1676..>2069
                     /locus_tag="AC16HB_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5606585.1"
                     /translation="MPEGDHSLLSRLLAGDRRALARAISAVENGTPAAASIGAGIESK
                     LGRTHVVGITGAPGAGKSTLINALLGELIRRGRTIAVVAVDPSSPLTGGAVLGDRVRM
                     GEHGAHERVFIRSIASRGHLGGVSKTTAQ"
BASE COUNT          333 a          715 c          711 g          310 t
ORIGIN      
        1 aacccgtcga cgaccgactg ctgcgcgccg ccgacgccga gacgcagtgg caacgggaat
       61 tcacggagca gatcggcacc gatcgcgagg tcctcaatcg ctccggcatc ggcatcaagg
      121 cgctgtacac gaatcgcgac tgggattcgt cgcgccacgc aaacgatctc ggctatccgg
      181 gacagcttcc gtacacgcgt ggcatctacc caacgatgca tcgcggccgc acgtggacac
      241 agcgacagct gatcgggctg ggcacgcccg acgactacaa cgcgcgcctg cgcgcggtcc
      301 tcgcgcaggg cggcaatgcg gtgtcgctga ttccgtgcaa ctcggtgttc cgtggcgtcg
      361 acatggacga gtcgcacttc gaactgctcg gcacgtgcgg cacggtggtc aactccgtcg
      421 agcacatgga tcgggcgctc gccggcgtcg atctcgcccg cacgtcctgc gcgatgaacg
      481 acccgtcgcc gttcacgctg ctggcattca tgctcgcggt ggcgaagcgg cgcggcgtcg
      541 actggcgcgc gatcaccggc acgtccaacc agagcgacta cctgagccat tacgtcgcca
      601 accacatgtt ctttcgcatc gcgctgccgg gggcgcggcg gatcctcggc gaccacatcg
      661 acttctgcaa ccgcttcgtg ccgaggtgga acccgatgtc ggtggtgggc cagcacatgc
      721 agcaggccgg cgccacgccc gccgaggcca tggcgttcac gctcgcgacc gcgctgcagg
      781 tcgcgcagga ttgcatcgac cgcggcatgg ccccggactc attcctgccg cgcttcacgt
      841 tcttcttcga catctcgctg tcgttcttcg aggaagtcgc caagttccgc gccggccgcc
      901 gcatctgggc gcggctcgcg cgcgagcggc tcggggcgca ggaaccgcgc tcatggcgat
      961 tcaaattcca cggccagacc tcgggcgtgg acctgacccg ccagcagccg ctgaacaaca
     1021 tcgcgcgcgt gaccgtgcag gcgatggccg gcatcttcgg cgggctgcag tcgctgcaca
     1081 ccgacgcgtt cgacgaggcg ctgtcgacgc cgaccgactt cggcgcgcgc atcgcggtca
     1141 acacgcagaa catcctgcgc gaggaggcgc atctgacgga cgtgatcgat ccgctcggcg
     1201 gcagcttcta tgtcgagaag ctgaccgacc agatggaaga ggagatcctg cgcgtgatcc
     1261 ggatcgtcga cgacgcgggc ggcatgtacc gggcggtcga agcggggctg gtgcagaaga
     1321 tgatcggcga gtccgcgcgc cgcttccagc agcaggtcga gtccggcgcg cagaccgtcg
     1381 tcggcgtcaa tgcctacacc gccgcggagg acggcagcgc gcgccagccg ctggcacgcc
     1441 ccgatgcggc cacgatgcgc gcgcacctgg aggcgttcag ggcgtacaag gcgctgcgct
     1501 cgcaggcagt cgttgattcc gcactctccg cactggcgcg tgcggccaac gacccgcggg
     1561 acaacgtatt cgcgcaggtc atcgcggccg cggaggccgg ctgcacgcat ggcgagatct
     1621 gcggcacgct gcggcgcgag ttcggcttcg ggcacgtgca ggcgttcgtc tgaacatgcc
     1681 cgaaggcgat cactcgttgc tgtcgcgact gctcgccggc gaccgccgcg cgctggcgcg
     1741 cgcgatctcg gcggtcgaga acggcacgcc ggcagcggca tcgatcggcg ccggcatcga
     1801 gtcgaagctc ggccgcacgc acgtggtcgg catcaccggc gcgcccggcg cgggcaagtc
     1861 gacgctgatc aacgcgctgc tcggtgagct gatccggcgc ggccggacga tcgcggtagt
     1921 cgcggtcgat ccttcgagcc cgctgaccgg cggcgcagtg ctcggcgacc gcgtgcgcat
     1981 gggcgagcac ggtgcgcacg agcgcgtatt catacgctcg atcgcatcga gaggtcatct
     2041 cggcggcgtg tcgaagacga ccgcgcaga
//
LOCUS       DBNHBY010000287         1665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1437561, whole genome shotgun sequence.
ACCESSION   DBNHBY010000287 DBNHBY010000000
VERSION     DBNHBY010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1437561"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16HB_07055"
     CDS             <1..477
                     /locus_tag="AC16HB_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5606586.1"
                     /translation="ASPRLLLARLNRLTSAITSAIALPPQLAVGGLRLDAESREARTS
                     DLHLGLTASEFDVLFALASHVERPVSRAAIARALGRDAGTGRAVDMAVCRIRRKLREA
                     SVTGVRIRTSYGRGYVLSVEGRAADADPWPAVAAGARRDAPGGAPSKLFDLAALPA"
     gene            complement(482..922)
                     /locus_tag="AC16HB_07060"
     CDS             complement(482..922)
                     /locus_tag="AC16HB_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5606587.1"
                     /translation="MSVLDHYKNRGFECMCGNLRMAARAITRMYDEELAPCGLRASQL
                     AVLWAATALEPASVQSIAQTIAMDQSTLIRNLRLLERAALVSITTGSDRRSRIVRTTV
                     GGRRRFERAMPLWKNAQRRLARALKQRHAGMGARTLLRMARAFG"
     gene            complement(1042..>1665)
                     /locus_tag="AC16HB_07065"
     CDS             complement(1042..>1665)
                     /locus_tag="AC16HB_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR5606588.1"
                     /translation="VDLKSGGYLIIDQTEAMTTIDVNTGGYVGTRNFDDTIFKTNLEA
                     AQAIARQLRLRNLGGIIVVDFIDMTSAEHRDAVLAEFKKALARDRTRVTVSGFTQLGL
                     VEMTRKRTRESLAHILCEPCPTCGGRGEIKTARTVCFEILREILREARQFAETREFRI
                     LASQTVVDLFLEEESQSLAMLSDFIGKPISMQVETVYHQERYDIVLM"
BASE COUNT          235 a          574 c          587 g          269 t
ORIGIN      
        1 gcgtcgccgc gactgctgct ggcgcggctg aaccggctga cttcggcgat aacctcggcg
       61 atcgcgctgc cgccgcagct cgcggtgggc ggtctgcgcc tggacgccga atcgcgtgag
      121 gcgcgcacca gcgacttgca tctggggctc actgcgagcg aattcgatgt gctgttcgcg
      181 ctggcgagcc acgtcgaaag gccggtgagc cgcgctgcga tcgcccgcgc gcttggccgc
      241 gacgcgggca cgggccgcgc ggtcgacatg gcggtgtgcc gcatccgccg caagctgcgc
      301 gaagccagcg tcacgggggt ccggatccgg acctcctatg gccgcggata cgtgctgtcg
      361 gtcgagggcc gggcggccga tgcagatccg tggcccgcgg ttgcggcggg cgcgcggcgc
      421 gacgcgcctg gtggggcgcc cagcaaactg ttcgatctgg ccgcgctgcc ggcctgacgc
      481 ttcagccgaa cgcgcgcgcc atgcgaagca gcgtgcgcgc gcccatgccg gcatggcgct
      541 gcttcagcgc acgcgcaagc cggcgttgcg cgttcttcca caagggcatg gcgcgctcga
      601 agcggcgccg tccgccgacg gtggtccgca cgatgcggct gcgacggtcg ctgccggtcg
      661 tgatcgaaac gagcgcggcg cgctcgagca gccgcaggtt gcgaatcagc gtcgactgat
      721 ccatcgcgat ggtctgcgcg atcgactgca cgctcgccgg ttcgagcgcg gtggccgccc
      781 acagcaccgc cagctggctc gcgcgcagcc cgcagggcgc gagttcctcg tcgtacatgc
      841 gcgtgatcgc gcgcgcggcc atgcgcaggt tgccgcacat gcattcgaag ccgcggttct
      901 tgtagtggtc caggacgctc atcgggaggc acgcgctgcg gatccgggtg gcggacagga
      961 tggcgccaaa tcgttcgcgc cgcacgccgc accgcaaccg aaacgcggca gccgcgctcg
     1021 attgtcacag cgacgtcgcc gctacatcag cacgatgtcg tagcgctcct ggtggtagac
     1081 ggtctcgacc tgcatcgaga tcggcttgcc gatgaagtcc gacagcatcg ccagcgattg
     1141 cgactcctcc tccaggaaca ggtcgaccac cgtctgcgac gccaggatgc ggaactcgcg
     1201 cgtctcggcg aactggcgcg cctcgcgcag gatctcgcgc aggatctcga agcacacggt
     1261 gcgcgcggtc ttgatctcgc cgcgcccgcc gcaggtcggg cacggctcgc acaggatgtg
     1321 cgccagcgat tcgcgcgtgc gcttgcgcgt catctcgacc agtccgagct gcgtgaagcc
     1381 gctcaccgtg acgcgcgtgc ggtcgcgcgc cagtgccttc ttgaattcgg ccagcaccgc
     1441 atcgcggtgc tcggcgctcg tcatgtcgat gaagtcgacc acgatgatgc cgcccaggtt
     1501 gcgcaggcgc agctggcgcg cgatcgcctg cgccgcctcg agattggtct tgaagatcgt
     1561 gtcgtcgaaa ttgcgcgtgc cgacgtagcc gccggtgttg acgtcgatcg tcgtcatcgc
     1621 ctcggtctgg tcgatgatca ggtagccgcc gctcttcagg tccac
//
LOCUS       DBNHBY010000288         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1641050, whole genome shotgun sequence.
ACCESSION   DBNHBY010000288 DBNHBY010000000
VERSION     DBNHBY010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1641050"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="AC16HB_07070"
     CDS             complement(<1..504)
                     /locus_tag="AC16HB_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5606589.1"
                     /translation="MNATAVVAEDEATLRLQLVEQLGQLWPELAIVGEASDGVQALRL
                     LDEHRPDILFLDIQMPGATGLEVARQASGRSRVVFVTAYDEHAVAAFEHGAIDYVLKP
                     ISAARLFTTVSRLKERLGTPPARLDGVLAQIAPAAKTYLRWINASVGQNLRLLTVEEI
                     QYFQADNK"
     gene            complement(501..1628)
                     /locus_tag="AC16HB_07075"
     CDS             complement(501..1628)
                     /locus_tag="AC16HB_07075"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR5606590.1"
                     /translation="MNALASDVLASGADAATTEPRSIFAILRSVTWREVGFFLLVALL
                     IAAINATSYFEFAAKEQAAMKLLTGIVLPMLVVPFCVCAWLLADRGSDAVLPRLPRMI
                     VCVAVAALLAALLIPHVVELFGLQFNGEIKYDGKSMHLPDWMMQLSFGLDVAFFTGLS
                     FAVLEMSGRRRSAERAVAAEQREQAALARQLLESRLAAMQAQVEPQFLFDALVDIERL
                     YARDANAAAGNLDRLIQYLRVALPRLRESGSSIQAEVELVESYLAVVQALNEGRPALA
                     KWIEREAAYRTFYPMLLLPLIQRAVRRDEQAPATIDLEVAHRDGGVRIGVRIAAGGLC
                     TGNGELDRVRERLHGLYGGRARLDCAEPDAHTTEFTLLLPA"
     gene            complement(1625..2767)
                     /locus_tag="AC16HB_07080"
     CDS             complement(1625..2767)
                     /locus_tag="AC16HB_07080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5606591.1"
                     /translation="MATATSADPDRAGFEWRAERFRHEFFRFFNFGTVAAVFFICLAV
                     AFTRWLPTLYFDAPSTWALDGIRYLRQTLISGLCALAAIALVQALARAFDWSQRRTYA
                     VGVIAIVIAAAVGAVARLLVYGLPFSQVHERWTWGASIVGLWSLVGGLGFTLAHFAGE
                     EAGARKRLGDAASARETLDAQMVQARLSALQAQIEPYFLFNTLANVKRLYETAPQRGR
                     EMLVSLIDYLRAALPSMRQTGSTLKRELELARAYLTILQMRMGERLRFTIDVEPRLLA
                     AEMPPLLLGTLIENAIKHGLSPLPEGGSIAIRAQRSGDEVRIEVRDTGRGFTGHGGSG
                     VGLANIRSRLAALYGARAALELSANTPCGVVATVALPWRAAAEVPA"
BASE COUNT          480 a         1012 c          984 g          402 t
ORIGIN      
        1 cttgttgtcg gcctggaagt actggatctc ttcgaccgtg agcagccgca ggttctgtcc
       61 gaccgacgcg ttgatccagc gcaggtacgt cttggcggcc ggagcgatct gcgccagcac
      121 gccatcgagg cgcgccggcg gcgtgccgag gcgctccttg agccggctca ccgtcgtgaa
      181 caggcgcgcg gcggagatcg gcttcagcac atagtcgatc gcaccatgct cgaaggcggc
      241 caccgcatgc tcgtcgtacg cggtgacgaa caccacgcgg ctcctgccgc tcgcctgccg
      301 cgcgacttcg aggccggtgg cgccgggcat ctggatgtcg aggaacagga tgtcgggtcg
      361 gtgctcgtcg agcagacgca gggcctgcac gccgtcggac gcctcgccga cgatcgccag
      421 ttccggccac agctggccga gttgttcgac cagttgcagg cgcaaggtgg cttcgtcctc
      481 ggcgacgacc gcggttgcgt tcatgccggc aacagcagcg tgaactcggt cgtatgcgcg
      541 tccggctccg cgcaatcgag ccgcgcccgg ccgccgtaca gcccgtgcaa ccgttcgcgc
      601 acgcggtcca gctcaccgtt gccagtgcac agcccgccgg cggcgatgcg cacgccgatg
      661 cgcacgccgc cgtcgcggtg cgcgacttcg agatcgatcg tggccggtgc ctgctcatcg
      721 cgccgcactg cgcgctggat caacggcagc aacagcatcg gatagaacgt gcggtaagcg
      781 gcttcgcgct cgatccactt ggcgagcgcc ggccgaccct cgttgagcgc ctgcacgacc
      841 gcgaggtatg actcgaccag ttcgacctcg gcctggatgc tcgaacccga ttcgcgcaaa
      901 cgcggcagcg ccacccgcag gtactggatc aggcggtcga ggttgccggc ggcggcgttt
      961 gcatcccgcg cgtagagccg ctcgatgtcc accagcgcat cgaacaggaa ctgcggctcg
     1021 acctgcgcct gcatcgcggc caggcgcgat tcgagcagct gccgggcgag cgcagcctgt
     1081 tcgcgctgct cggccgcgac cgcgcgctcg gcgctccggc gccgcccgct catctcgagc
     1141 accgcgaacg acaggccggt gaagaatgcc acgtcgagcc cgaagctcaa ctgcatcatc
     1201 cagtccggca ggtgcatcga cttgccgtcg tacttgatct cgccgttgaa ctgcaagccg
     1261 aagagctcga cgacatgggg tatcagcagc gccgccagca gcgccgcgac ggcgacgcac
     1321 acgatcatgc gcggcagccg cggcagcacg gcatcgctgc cgcggtcggc gagcagccag
     1381 gcacagacgc agaaaggaac caccagcatc ggcagcacga tgccggtcag cagcttcatc
     1441 gccgcctgct ccttggcggc gaactcgaag tagctggttg cgttgatcgc ggcgatcagc
     1501 agtgcgacga gcagaaagaa cccgacctcg cgccaggtca cgctgcgcag gatcgcgaag
     1561 atgctgcgcg gctcggtggt cgcggcgtcc gcgccgcttg cgaggacgtc ggacgccagc
     1621 gcgttcatgc cggcacctcc gctgccgcgc gccacggcag cgcgaccgtc gcgaccacgc
     1681 cgcacggtgt gttggcggaa agctccagcg ccgcgcgcgc gccgtacagc gccgccagcc
     1741 gcgaacggat gttggcgagt ccgacgccgc tcccgccgtg accggtgaag ccgcgcccgg
     1801 tgtcgcgcac ctcgatgcgc acctcgtcac cgctgcgctg cgcgcggatc gcgatgctgc
     1861 cgccctcggg cagcggcgac aggccgtgct tgatcgcatt ctcgatcagc gtgcccagca
     1921 acagcggcgg catctcggcg gccaggagcc gcggctcgac atcgatcgtg aagcgcaacc
     1981 gttcgcccat ccgcatctgc aggatcgtga ggtacgcgcg cgccagctcg agttcgcgct
     2041 tcagcgtcga gccggtctgc cgcatcgacg gcagcgcggc gcgcaggtag tcgatcaggc
     2101 tgaccagcat ctcgcgcccg cgctgcggcg cggtttcata cagccgcttc acgttggcga
     2161 gggtgttgaa caggaagtac ggctcgatct gcgcctgcag cgccgacagg cgcgcctgca
     2221 ccatttgcgc gtcgagcgtc tcgcgtgcgg aggcggcatc gcccaggcgc ttgcgcgcgc
     2281 cggcctcctc gcccgcgaag tgcgccagcg tgaaccccag gccgccgacc aatgaccaca
     2341 ggccgacgat gctggcgccc caggtccagc gttcgtggac ctgcgaaaaa ggcaggccgt
     2401 agacgagaag ccgcgcgacg gcaccgactg cggcggcgat gacgatcgcg atcacgccca
     2461 ccgcgtacgt gcgccgctgc gaccagtcga acgcgcgcgc cagcgcctgc accagcgcga
     2521 tcgcggccag cgcgcacaag ccgctgatca gggtctgccg cagatagcgg atgccgtcca
     2581 atgcccaagt cgacggcgcg tcgaagtaca aggtcggcag ccagcgcgtg aaagcgaccg
     2641 ccaggcagat gaagaacacg gccgccacgg tgccgaaatt gaagaagcgg aagaattcgt
     2701 gccggaagcg ttcggcgcgc cattcgaatc cggcgcggtc ggggtcggca gaggtggcgg
     2761 tcgccatggc agagtcggca agcggagcga ttttcccgcc atgcccacgc cggcggcaag
     2821 ggtgcgacga accggcccgg cgcggcgacg aactcgcgct aatgcagacg aaagggtg
//
LOCUS       DBNHBY010000289         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1506159, whole genome shotgun sequence.
ACCESSION   DBNHBY010000289 DBNHBY010000000
VERSION     DBNHBY010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1506159"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16HB_07085"
     CDS             <1..479
                     /locus_tag="AC16HB_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5606592.1"
                     /translation="LAGELFKLQTKTFVVHIPYRGAGPALQDLISGNVDMMFDGLGSS
                     AQHIRAGRIRALAVAAPKRSPGAPDVPTTVEAGLPEYQVSTWYGMWGVKGTQKDILDR
                     MHAEVVKALQTPELREIWASQGSEPLTMSPADFARFLNAEIKRWAQVAKASGAKLD"
     gene            510..>1930
                     /locus_tag="AC16HB_07090"
     CDS             510..>1930
                     /locus_tag="AC16HB_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5606593.1"
                     /translation="MKNENLYALIESRLPRDLAAPCIETADGLVYSWRDLHFASARIA
                     GWLASLELPRDARVLVQVEKSPEALMLYLATLRAGLAYVPLNTAYRQAELEYFLGDAR
                     PSVVVCAPGREREIGALARNSGCAQVMTLGEGRDGSLLEAAAPFADAFATAPRAASDV
                     AAILYTSGTTGRSKGAMLSHGNLSSNALVLDEFWGFKEEREAGGHDVLLHALPLFHVH
                     GLFVASHAALLGGAQMIFLPKFDPKQVLRYLPRSTVMMGVPTYYTRLLAEPSFDRHVC
                     RNMRLFISGSAPMLRETFEEFRRRCGHTILERYGMSETIMLTSNPYFGTARDRIGGTV
                     GMPLPGVAVRVVRDDGSRCATGEIGHVQVKGPNVFSGYWRMPEKTAEEFAADGFFKSG
                     DVGYFGPANPDERKPANYLTLVGRSKDLIISGGYNVYPKEIESYIDEMAGVVESAVIG
                     LPHPDFGEAVAAVVVGAPGATVDG"
BASE COUNT          291 a          632 c          691 g          316 t
ORIGIN      
        1 atctggccgg ggaactgttc aagctgcaga ccaagacctt cgtcgtgcac attccctatc
       61 gcggcgccgg gccggcgctg caggacctga tctccggcaa cgtcgacatg atgttcgacg
      121 gtctcgggtc gtcggcgcag cacattcgcg ctggccgcat ccgcgcgctc gcggtcgcgg
      181 cgccgaagcg ttcgcccggt gccccggacg tgccgaccac ggtggaggcc ggcctgccgg
      241 agtaccaggt atcgacgtgg tacggcatgt ggggcgtgaa gggcacgcaa aaggacattc
      301 tcgatcgcat gcacgccgag gtcgtgaagg cgctgcagac gccggaactg cgcgagatct
      361 gggcgagtca gggatcggag ccgttgacga tgagtccggc ggacttcgcc cgtttcctca
      421 atgccgagat caagcgctgg gcgcaggtgg ccaaggcttc cggcgccaag ctcgactgac
      481 ggtgcacggc ggcaacgccg cagcggatca tgaagaacga aaacctgtac gcgctgatcg
      541 agtcgcggct gccgcgtgat ctcgcggccc cgtgcatcga aaccgccgac gggctcgtct
      601 actcgtggcg cgacctgcac ttcgcttcgg cgcgcatcgc cggctggctc gcttcgctcg
      661 aacttccgcg cgacgcgcgc gttctggtgc aggtggaaaa gtcgcccgag gcgctgatgc
      721 tgtatctggc cacgctgcgc gcggggctcg cgtatgtgcc gctgaacacc gcgtaccggc
      781 aggccgagct cgagtacttc ctcggcgatg cacggccgtc ggtggtcgtg tgtgcgccgg
      841 gacgcgagcg cgagatcggc gcgctcgcgc gcaattccgg ctgcgcgcaa gtgatgacgc
      901 tcggcgaggg ccgcgacggc tcgctgctcg aggccgcggc gccgttcgcc gacgcgttcg
      961 cgaccgcgcc gcgcgcggcg tccgacgttg cggcaatcct gtacacgtcg ggtacgaccg
     1021 ggcgcagcaa gggggcgatg ctgtcgcacg gcaatctgtc gtccaacgcg ctcgtgctcg
     1081 atgaattctg gggcttcaag gaggagcgcg aggccggcgg ccacgacgtg ctgctgcacg
     1141 cgctgccgct gtttcacgtg cacggcctgt tcgtcgcgtc gcacgcggcg ctgctcggcg
     1201 gcgcgcagat gatcttcctg ccgaagttcg atccgaagca ggtgctgagg tacctgccgc
     1261 ggtcgaccgt gatgatgggt gtgccgacct actacacgcg gctgctcgcg gagccgtcgt
     1321 tcgaccggca cgtgtgccgc aacatgcggc tgttcatctc cggctccgcg ccgatgctga
     1381 gggagacctt cgaggagttc cgccgccgct gcggccacac gatcctcgag cggtacggca
     1441 tgtccgagac gatcatgctg acgtcgaacc cgtacttcgg cactgcgcgc gaccgcatcg
     1501 gcggcacggt cggcatgccg ctgccgggcg tggctgtgcg cgtggtgcgc gacgacggat
     1561 cgcgctgcgc gaccggcgag atcggccacg tgcaggtcaa gggaccgaac gtgttctccg
     1621 gctactggcg catgccggag aagaccgccg aggagttcgc ggccgacggc ttcttcaagt
     1681 ccggcgatgt cggctacttc ggacccgcga atccggacga gcgcaagccc gccaactacc
     1741 tgacgctggt cggccgctcg aaggacctga tcatctcggg cggctacaac gtgtacccga
     1801 aggagatcga gagctacatc gacgagatgg ccggcgtggt cgagtcggcg gtgatcggcc
     1861 tgccgcatcc ggacttcggc gaagcggttg cggcggtcgt cgtcggtgcg ccgggcgcga
     1921 cggtcgacgg
//
LOCUS       DBNHBY010000290         8900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_17054, whole genome shotgun sequence.
ACCESSION   DBNHBY010000290 DBNHBY010000000
VERSION     DBNHBY010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_17054"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1034)
                     /locus_tag="AC16HB_07095"
     CDS             complement(177..1034)
                     /locus_tag="AC16HB_07095"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5606594.1"
                     /translation="MTPSPRTAFVLAGGGSLGAVVVGMLREIVAAGERPDLVVGASAG
                     AINGAYYAANPTPDGVAALEDLWRSLTGTRVFGPRWMSLMALLRRRDHLFGTARLRQL
                     LEKHLSFGDLRATRVQLHVVASDQRTGEEVILSEGNVVDAVLASSAIPGVFPAVRIGT
                     RELVDGGVASNTPIGAAIRLGATRLVVLPTGFTCDQKRVPTDALGRTMHALSLLVTRQ
                     LVVDLERWSAHAQIVVAPPLCPLDCSPYDYSHGAELIERAAASTRAWIERDGLTTPGI
                     PHQLVEHRH"
     gene            complement(1047..1826)
                     /locus_tag="AC16HB_07100"
     CDS             complement(1047..1826)
                     /locus_tag="AC16HB_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606595.1"
                     /translation="MAKAWAKFPYHDKRFVYSAASVKKAWDRLHRGDCEPWPNGSAMV
                     EAWIAFHAGEFQKAADAGLKAGGAGMTVANKATAIYANYLEENEKTKLALFEEVAARA
                     ETQQKTEPKNANAFYWHAYALGRYAQGISIVKALAQGIGTKVRASLEMTLRLQPKHAD
                     AHIALGSYHAEVIDKVGAMVGGLTYGAKKDAGLKHFETALKLNPDSAIARIECANGLV
                     MLEGKKALAKAEKLYEEAAAFEPLDAMEKLDVELAKAELED"
     gene            complement(1847..2506)
                     /locus_tag="AC16HB_07105"
     CDS             complement(1847..2506)
                     /locus_tag="AC16HB_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5606596.1"
                     /translation="MERKPPRRTRERILELAQRLFNDFGEPNVNTTLIAEEMNISPGN
                     LYYHFKNKEDIINSIFQQFEREADRLLTVPGDRRPNVEDAWLFLHLLFELIWKYRFFY
                     RDLNNLLINNRTFEMRFKTLLGQKTRMARWLTEGLAQQGELKGTRAELEALATNMVVV
                     ATYFLSYEYVLNPRRFSEPEVVGAALARGCYQVMALTAPYLVGNGRALFEKLAAEYLK
                     K"
     gene            complement(2568..3782)
                     /locus_tag="AC16HB_07110"
     CDS             complement(2568..3782)
                     /locus_tag="AC16HB_07110"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5606597.1"
                     /translation="MARIGLALAGGGPLGAIYEIGALAAVSEALRGIDFTDADVYVGV
                     SSGGFIAAGLVNGFTPHQMSRLFIEGHKSEDRFDPSMLLRPALREYLTRAWSLPPLLA
                     MALWNYVARPGNLMGSIERLGRAIPTGIFDGDAIDKYLGRIFAQTGRSNDFRELRHKL
                     YLVATDLDTGEAVAFGTRGFDHVPVSRAVQASAALPGLFPPVEIGGRYFVDGALRKTL
                     HASIALEHGVDLLLCLNPLVPYDARPHHPTRDRAHRLDKLVEGGLPVVLAQTFRSIIH
                     SRLGAGMERYKTAYPGTDIVLFEPNRADADMFFTNLFSYSSRRRLAEHAYQKTRRELW
                     ERRHELGPKLARHGIEIDVACLRNEKMTLVKSLRRTRELRLETVSSITARELKHTLDD
                     LERWLGAGAVAA"
     gene            complement(4007..4465)
                     /locus_tag="AC16HB_07115"
     CDS             complement(4007..4465)
                     /locus_tag="AC16HB_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5606598.1"
                     /translation="MVRKLKAMAGRAGKDGNLAAAIKDSAQQIWLAGLGAFSKAQEEG
                     TKVFEALVREGNSIQKRTMQMTEDKVSEVTNRVAKVAGEVQKQANQSWDKLESVFEER
                     VERAMKRLGVPTSREVHALTRRVEELTASVHKLGGKPARKSTARARKAAA"
     gene            4657..6669
                     /locus_tag="AC16HB_07120"
     CDS             4657..6669
                     /locus_tag="AC16HB_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606599.1"
                     /translation="MEYFVTGASGFIGKRLVRKLLARKGAVVHFLLREESRDKVSALL
                     ADWGVDEKRVRPVYGDLAAPKLGMAKADSKALAGRIDHFFHLAAIYDLKADAESQIRV
                     NIEGTRNAVQLANELKVGCFQHVSSIAAAGMYEGVFREDMFDEAENLDHPYFATKHDS
                     EKIVRKECRTPWRVYRPGLVVGDSKSGEMDKIDGPYYFFKLIQRMRQMLPPWMPTIGI
                     EGGRINIVPVDFIVDAMDHIAHAKGHDGKAFHLVDPTPYRVGDVLNIFARAGHAPQFA
                     LRINAALFGFIPNSVKKGMLALTPVRRIRNAVLKDLGLPEDMLQFVNYPTRFDAREAT
                     AALKGTGIECPRLEDYAYRLWDYWERNLDPDLFIDRTLKGQVAGKVVLVTGGSSGIGL
                     AAAHKIAAAGAITVIVGREQDKLDAAKKEIDSALKESGNTNRGQVVTFSADLASMESC
                     DKMVAWLVENYGGVDVLINNAGRSIRRGIESSYDRFHDFERTMQLNYFGCLRITMGLL
                     PHMVAKHRGHVVNISSIGVLTNAPRFSAYVASKAALDAWTRCAASEFIDVGVTFTTIN
                     MPLVRTPMIAPTRIYQNVPTLSPEAAADLIVQAIVYRPVRIATRLGVFGQVLHALAPR
                     VAQITMNTTFRMFPDSAAAKGAKEGEKPKATPDQIALANLMSGIHF"
     gene            6741..7769
                     /locus_tag="AC16HB_07125"
     CDS             6741..7769
                     /locus_tag="AC16HB_07125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5606600.1"
                     /translation="MTKVTPPATIELVPRSGPFTLSCVVPVHNEADIVAKFLGALRQT
                     AAALTPRFEIIVVNDGSTDTTGAVVRALAPELALTLIEFSRNFGKEAALTAGLDLARG
                     DCVLLIDADFQHPLQSVVDMVNAWRAGYDMAYGLRTDRSGESWLKRAGTRVFYRMMAA
                     SQSINIPPDAGDFRLMDRRVVDALRRLPERHRFMKGLYAWVGFRSVGIPFEVKARAGG
                     TTSFSMRRLQSLALTGITAFSNLPLRIGGMAGAVIAAFAFLWGGWIVIERIFFGQPIQ
                     GFATLAASVLFFAGVQLISIGVLGEYLGRVYDEVKARPTYVVARLDDHSPLRDAETSL
                     PQQHLAHQ"
     gene            7778..8146
                     /locus_tag="AC16HB_07130"
     CDS             7778..8146
                     /locus_tag="AC16HB_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR5606601.1"
                     /translation="MVRFGMVGAAAAATHFVVAVACVRGLSLDPQLANVVGFLVAFAL
                     SFLGQWRWTFGAHAAPLARALPSYFLVALTGFAANALAYRWLLMHTPLRYDVALALVL
                     IAVAAMTFVLSRFWAFRRVR"
     gene            8146..>8900
                     /locus_tag="AC16HB_07135"
     CDS             8146..>8900
                     /locus_tag="AC16HB_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR5606602.1"
                     /translation="MARRVALCADDYGYNAAVDQAILVLIDQRRLGGVSCMVDAPRFA
                     EAFAPLREREREFDIGLHFNLTETFPDAPRVGTLPVVIGLAWLHGLGANDIMLRLRRQ
                     LAKFESAFKRMPAYIDGHQHVHQFPVIRDALLRELDLRYGTRRVLIRNTASASDDPKM
                     RLLARLGGRTMKAHLQLLGWPTNLDFVGAYRYAPGADFAALAAGWIRTLKDGAIWMCH
                     PAARAEPGDAIGAFRVAEFKWLSSPNFDPERLPA"
BASE COUNT         1387 a         2943 c         3017 g         1553 t
ORIGIN      
        1 cacggatgaa gcattggcag gacgtggtgg gcgccgtacc gggcgcctgg ctgatcgtcg
       61 cgatcctcgc gctgtcggtg ctgctgaccg acaaggacta cagcggctgg tggcgcaagg
      121 ccgcgcattg aacgcccgtc ggccaggggc acgtgccgtg cccctgtctt cttcattcag
      181 tgccggtgtt cgaccagctg atgcggaatg cccggcgtgg tgagcccgtc gcgctcgatc
      241 catgcgcgcg tgctggcggc ggcacgctcg atcagttcgg cgccgtgcga atagtcgtac
      301 ggcgagcagt cgagcgggca cagcggcggc gcgacgacga tctgcgcgtg cgctgaccag
      361 cgctccaggt cgaccacgag ctggcgcgtg accagcaggc tcagcgcgtg catcgtgcgg
      421 ccgagcgcat cagtcggcac gcgcttctgg tcgcaggtga accccgtcgg cagcacgacc
      481 agccgcgtgg cgccgagccg gatcgcggcg ccgatcggcg tgttgctcgc cacgccgccg
      541 tcgacgagtt cgcgcgtgcc gatcctgaca gcgggaaaga cgccggggat cgcgctgctc
      601 gccagcacgg catcgacgac attgccctcg gacaggatca cctcttcacc ggtgcgctgg
      661 tcgctcgcca cgacatgcag ctgcacccgt gtcgcgcgca ggtcgccgaa tgaaaggtgc
      721 ttctcgagca gctggcgcag ccgtgccgtg ccgaagagat ggtcgcgccg ccgcagcagt
      781 gccatcagcg acatccagcg cgggccgaag acgcgcgtgc cggtcaggct gcgccagagg
      841 tcttcgaggg cggccacgcc gtccggcgtc ggattggccg cgtagtaggc gccgttgatc
      901 gcgccggccg aggcgccgac gacaagatcg ggccgctcgc cagccgcgac gatctcccgc
      961 agcatgccga ccaccacggc gccgaggctg ccgccgcccg ccagcacgaa cgcggtgcgc
     1021 ggagacgggg tcatgaccgt cgacggtcag tcctccagct cggccttcgc cagctcgacg
     1081 tcgagcttct ccatcgcgtc cagcggctcg aacgcggcgg cctcttcata cagcttttcc
     1141 gccttcgcca gcgccttctt gccttcgagc atcaccaggc cgttcgcgca ctcgatgcgc
     1201 gcgatcgccg aatccggatt cagcttcagc gctgtctcaa agtgcttcag ccccgcgtcc
     1261 ttcttcgcgc cgtaggtcag tccgcccacc atcgcgccga ccttgtcgat gacctcggcg
     1321 tggtaggagc cgagcgcaat gtgcgcatcg gcgtgcttgg gttgaagcct gagcgtcatc
     1381 tccaggctcg cgcgcacttt cgtgccgatg ccttgcgcca gcgccttgac gatcgagata
     1441 ccctgcgcat agcggccgag cgcataggcg tgccagtaga acgcattggc attcttcggc
     1501 tccgtcttct gctgtgtctc ggcgcgtgcc gccacttcct cgaacagcgc gagcttggtc
     1561 ttctcgtttt cctcgaggta attggcgtag atcgccgtgg ctttgttggc gaccgtcatg
     1621 ccggctccgc ccgccttcag tcccgcgtcg gcggccttct ggaattcgcc ggcgtgaaac
     1681 gcgatccacg cctcgaccat ggcagaaccg ttcggccacg gctcgcagtc gccgcggtgc
     1741 agccggtccc acgctttctt cacgcttgcc gcgctgtaga cgaatctctt gtcgtgatag
     1801 gggaacttcg cccacgcctt ggccatgatc tctccttgcc tggacgctat ttcttcaggt
     1861 attccgccgc cagcttctcg aacagagcgc gcccgttgcc gaccaggtac ggcgcggtca
     1921 gcgccatcac ctgatagcag ccgcgtgcca gcgccgcacc cacgacttcc ggttcgctga
     1981 agcggcgcgg attcagcacg tactcgtacg acagaaagta ggtcgcaacc acgaccatgt
     2041 tggtcgccag cgcttcgagt tcggcgcgcg ttcccttcaa ctcgccctgc tgcgcgagac
     2101 cttcggtgag ccagcgcgcc atgcgcgtct tctgtccgag cagcgtcttg aagcgcatct
     2161 cgaacgtgcg gttgttgatc agcaggttgt tcagatcgcg gtagaagaag cggtacttcc
     2221 agatcagctc gaacagcagg tgcaggaaca gccacgcgtc ctccacgttc gggcggcggt
     2281 cgccgggcac ggtcagcaac cggtcggcct cgcgctcgaa ctgctggaag atgctgttga
     2341 tgatgtcttc cttgttcttg aagtggtaat acaggttgcc gggggagatg ttcatctcct
     2401 cggcgatcag cgtggtgttg acgttcggct cgccgaaatc gttgaacagg cgctgcgcga
     2461 gttcgaggat gcgttcgcgc gtgcggcgcg gcggcttgcg ttccatggac tggaagcggt
     2521 gttgttcttg acgggaggtt cgcggcgaga atagcaccgc gaactgatca ggccgctacc
     2581 gcgcccgcgc cgagccagcg ttcgaggtcg tccagcgtgt gcttcagttc gcgcgcggtg
     2641 atcgacgaga ccgtctccag ccgcagttcg cgcgtgcggc gcaggctctt caccagcgtc
     2701 atcttctcgt tgcgcaggca ggcgacgtcg atctcgattc cgtgccgcgc cagtttcgga
     2761 ccgagctcgt ggcggcgctc ccacagttcg cgccgcgtct tctggtacgc gtgctcggcc
     2821 aggcgccgcc ggctcgaata cgagaacagg ttcgtgaaga acatgtccgc gtccgcgcga
     2881 ttcggctcga acagcacgat gtcggtgccc gggtacgcgg tcttgtagcg ctccatgccg
     2941 gcgcccagcc gcgaatggat gatcgagcgg aaggtctgcg ccagcacgac cggcagtcca
     3001 ccttcaacca gcttgtcgag ccggtgcgcg cggtcgcgcg tcgggtgatg cggtcgcgcg
     3061 tcgtacggca cgagcggatt caggcagagc agcagatcga cgccatgctc cagcgcgatc
     3121 gaagcgtgca gcgtcttgcg cagcgcgccg tcgacgaagt agcgcccacc gatctcgacc
     3181 ggcgggaaca gtcccggcag cgcggctgac gcctgcaccg cgcgcgagac cgggacgtgg
     3241 tcgaagcccc gagtgccgaa cgcgacggct tcacccgtgt cgagatcggt cgcgacgagg
     3301 taaagcttgt ggcgcagctc ccggaagtcg ttggagcggc cggtctgcgc gaagatgcgc
     3361 cccaggtact tgtcgatcgc gtcgccgtcg aagatgccgg tggggattgc tcggccgagt
     3421 cgctcgatcg agcccatcag attgccgggt cgggcaacgt agttccacag ggccatcgcc
     3481 agcagcggcg gcaacgacca ggcgcgcgtc aagtattcgc gcagcgctgg gcgcagcagc
     3541 atcgacggat cgaagcggtc ctcggacttg tgcccctcga tgaacaggcg cgacatctgg
     3601 tgtggcgtga agccgttgac caggccggcc gcgatgaatc cccccgagct gacgccgacg
     3661 tagacgtcgg catcggtgaa gtcgatgccg cgcagcgcct cggaaacagc cgcgagcgcg
     3721 ccgatttcat agattgcgcc cagcggtccg ccgcccgcga gcgcgagccc gatgcgggcc
     3781 acgcgcctcg agcgggagcg ggattcggcg gggccgccag gggcggtgga ccgttcgctc
     3841 atgcgctcga gcggaccgcg tggggcattg cgcgggcctg cgcgcgccgg gcgagcgagc
     3901 cccgcgacgg gcgcgcggcc gatgccttca tgcgaggcgt catcagaaga caaggccgcg
     3961 gggcttggcg ccgccgcggc cttgcacggt caaccgggga tcgggatcag gccgcggcct
     4021 tgcgagcacg cgcggtcgac ttgcgggccg gcttgccgcc gagcttgtgc acgctggcgg
     4081 tcagttcctc gacccgcctg gtcagtgcgt ggacttcccg gctggtcgga acaccgaggc
     4141 gcttcatcgc gcgctccacg cgctcctcga acaccgattc cagcttgtcc cagctctggt
     4201 tggcctgctt ctgcacttca ccggccacct tggcgactcg gttggtgacc tcgctgacct
     4261 tgtcttccgt catctgcatc gtgcgcttct ggatcgaatt gccttcgcgg accagcgcct
     4321 cgaagacctt ggtcccttct tcctgcgcct tggagaacgc gcccaagccg gccagccaga
     4381 tctgctgcgc cgaatccttg atcgccgcgg ccagattgcc gtccttgcct gcacgacctg
     4441 ccattgcctt cagcttcctg accatgatgt gctccttcgc gtggtttggt gcttctgtaa
     4501 aacgaagtct aggcagcgct gctagagccg ttgtactaat agcgtagagc ggggcgacta
     4561 ggtgcccgtc gccgcggcgc gcttgcggcg cgtggttaga atcgtccgca caagaccaac
     4621 attcggactc gctgcaagcg aaggaggaga ctcgaaatgg aatacttcgt caccggcgcg
     4681 tccggcttca tcggcaagcg tctggtccgc aagctgctgg cgcgcaaggg cgcggtggtg
     4741 catttcctgc tgcgtgagga aagtcgggac aaggtttcgg cgctgctggc cgactggggc
     4801 gtcgacgaga agcgcgtgcg cccggtgtac ggcgacctcg cggcgccgaa gctcgggatg
     4861 gcgaaagccg acagcaaggc gctggcgggc aggatcgatc acttcttcca tctcgcggcg
     4921 atctatgacc tcaaggccga cgccgagagc cagatccgcg tgaacatcga aggcacgcgc
     4981 aacgcggtgc agttggccaa cgagctgaag gtcggctgct ttcagcacgt gtcgtcgatc
     5041 gcggcggccg gcatgtacga gggcgtgttc cgcgaggaca tgttcgacga ggcagagaac
     5101 ctcgaccatc cgtacttcgc gaccaagcac gactcggaga agatcgtgcg caaggagtgc
     5161 aggacaccgt ggcgcgtgta tcggccggga ctggtggtcg gcgacagcaa gtcgggcgaa
     5221 atggacaaga tcgacggccc gtactacttc ttcaagctga tccagcgcat gcgccagatg
     5281 ctgccgccgt ggatgccgac gatcggaatc gagggcgggc gcatcaacat cgtgccggtg
     5341 gacttcatcg tcgacgcgat ggaccacatc gcgcatgcca agggccacga cggcaaggcg
     5401 ttccacctgg tcgacccgac gccgtaccgc gtcggcgacg tgctgaacat cttcgcgcgg
     5461 gcgggccatg cgccgcagtt cgcactgcgc atcaacgcgg cgctgttcgg cttcatcccg
     5521 aacagcgtga agaagggcat gctcgcgctc acgccggtgc gccggatccg caacgcggtg
     5581 ttgaaggatc tcgggctgcc cgaggacatg ctgcagttcg tcaactaccc gacccgcttc
     5641 gacgcgcgcg aggcgaccgc cgcgctgaag ggcaccggca tcgagtgccc gcggctggag
     5701 gactacgcct accggctctg ggactactgg gaacgcaacc tcgatccgga tctgttcatc
     5761 gaccgcacgc tgaaagggca ggtcgcgggc aaggtcgtgc tcgtcacggg cggctcttcg
     5821 ggcatcggac tggcggccgc gcacaagatc gccgctgccg gcgcaatcac ggtgatcgtc
     5881 gggcgcgagc aggacaagct cgacgccgcg aagaaggaga tcgactcggc gctgaaggaa
     5941 tccggcaata ccaacagggg ccaggtggtg acgttctcgg ccgatctggc atcgatggag
     6001 agctgcgaca agatggtcgc gtggctggtc gagaactacg gcggcgtcga tgtgctgatc
     6061 aacaacgccg gccgctccat ccggcgcggc atcgagtcca gttacgaccg cttccacgac
     6121 ttcgagcgga cgatgcagct gaactacttc ggctgcctgc gcatcacgat ggggctgctg
     6181 ccgcacatgg tcgccaagca tcgcggccac gtggtcaaca tcagctcgat cggtgtgctg
     6241 accaacgcgc cgcgtttctc ggcctacgtc gcgagcaagg ccgcgctgga cgcatggacc
     6301 cgttgcgccg cgagcgagtt catcgatgtc ggcgtgacct tcaccaccat caacatgccg
     6361 ctggtgcgca cgccgatgat cgcgccgacc cggatctacc agaacgtgcc gacgttgtcg
     6421 ccggaggcgg cggcggacct gatcgtgcag gcgattgtct accggccggt gcgcatcgcc
     6481 acccggctgg gcgtgttcgg ccaggtcctg cacgcgctgg cgccgcgcgt ggcgcagatc
     6541 acgatgaaca cgacgttccg catgttcccg gactcggccg cggccaaggg cgcgaaggag
     6601 ggcgagaagc cgaaggcgac cccggatcag atcgcgctgg cgaacctgat gagcgggatc
     6661 cacttctgag ggcgatgccg ggaccctgac atcgaggtat cgatgggcca gcccgaacgg
     6721 actgcgtgtc gagacgcatc gtgaccaagg taaccccgcc cgcgacgatc gagctcgtgc
     6781 cgcgcagcgg cccgttcacg ctgtcgtgcg tggtgccggt tcacaacgaa gcggacatcg
     6841 tcgcgaaatt cctcggtgcc ttgcgccaga ccgcggccgc gttgacgccg cgcttcgaga
     6901 tcatcgtcgt caacgacggc agcaccgaca cgaccggggc ggtcgtgcgc gcgctggcgc
     6961 cggagctggc gctgacgctg atcgagttct cgcgcaactt tggcaaggaa gcggcactga
     7021 cggcgggcct ggaccttgcg cgtggcgact gcgtgctgct gatcgacgcg gacttccagc
     7081 atccgctgca gagcgtggtc gacatggtca acgcgtggcg cgccgggtac gacatggcgt
     7141 acgggctgcg taccgaccgc tccggcgaat cgtggctgaa gcgcgccggc acgcgggtgt
     7201 tctaccggat gatggccgcg agccagtcga tcaacatccc gcccgacgcg ggcgacttcc
     7261 ggctgatgga ccggcgtgtg gtcgatgcgt tgcggcgtct gcccgagcgg caccggttca
     7321 tgaagggcct gtacgcatgg gttggcttcc gctcggtggg aattccgttc gaagtcaagg
     7381 cgcgcgccgg cgggacgacc tcgttcagca tgcgccgctt gcagtcgctt gcgctgaccg
     7441 gcatcacggc gttctccaac ctgcctctgc gcatcggcgg catggccggg gcggtgatcg
     7501 cggcgttcgc gttcctgtgg ggcggctgga tcgtgatcga gcgcatcttc ttcggccagc
     7561 cgatccaggg tttcgccacg ctggcggcct cggtgctgtt cttcgctggc gtgcagctga
     7621 tctcgatcgg cgtcctcggc gagtacctgg gccgcgtgta cgacgaagtc aaggcgcgac
     7681 cgacctacgt cgtggcgcgg ctcgacgatc acagcccgct gcgcgacgcc gagaccagtc
     7741 tgccgcagca gcatctggcg catcaatgat cgggcaggtg gttcgcttcg gcatggtcgg
     7801 cgccgccgcg gcagcgacgc acttcgtcgt cgccgtcgca tgcgtgcgcg gcctgtcgct
     7861 cgatccgcag ctggccaacg tggtcgggtt cctggtcgca ttcgcgctca gcttcctcgg
     7921 ccaatggcgc tggaccttcg gcgcgcatgc ggcgccgctg gcacgcgcgc tgccgtcgta
     7981 cttcctggtt gcgctgaccg gctttgcggc caacgcgctc gcgtatcgct ggctgctcat
     8041 gcacacgccg ctgcgctacg acgtggcgct ggcgctggtg ctgatcgcgg tggcggcgat
     8101 gacgttcgtc ctcagccgat tctgggcctt tcggagggtg cgctgatggc acgccgtgtt
     8161 gcgctgtgcg ccgacgacta cggctacaac gccgcggtcg accaggcgat cctggtgctg
     8221 atcgatcagc gacggctcgg cggcgtcagc tgcatggtgg acgcgccgcg cttcgccgaa
     8281 gcgttcgcgc cgctgcgtga acgcgagcgg gaattcgaca tcgggctgca cttcaacctg
     8341 accgaaacgt ttccggatgc gccgcgcgtc ggcacgctgc ccgtggtgat tgggctcgcc
     8401 tggctgcacg ggctgggcgc gaacgacatc atgctgcgcc tgcggcggca actggcgaaa
     8461 ttcgaaagcg cgttcaagcg catgccggcg tatatcgacg gtcaccagca cgtgcaccag
     8521 ttcccggtca tccgcgacgc gctgctgcgc gagctcgatt tgcgctacgg cacgcggcgc
     8581 gtgctgatcc gcaacacggc atccgcttcc gacgatccca agatgcgcct gctggcccgg
     8641 cttggagggc gcacgatgaa ggcgcacctg caactgctcg gctggccgac gaacctggac
     8701 ttcgtcggtg cctatcgcta cgcgcctggc gccgattttg ccgcgctggc cgccggctgg
     8761 atcaggacgc tgaaggacgg cgcgatctgg atgtgccacc cggccgcccg ggccgagccc
     8821 ggcgacgcca tcggcgcctt tcgcgtggcg gagttcaaat ggctgtcgtc gccgaatttc
     8881 gatccggagc gactgccggc
//
LOCUS       DBNHBY010000291         8384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_491280, whole genome shotgun sequence.
ACCESSION   DBNHBY010000291 DBNHBY010000000
VERSION     DBNHBY010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_491280"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1212
                     /locus_tag="AC16HB_07140"
     CDS             67..1212
                     /locus_tag="AC16HB_07140"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5606603.1"
                     /translation="MSEIPGGTTGWDGRTTLLDRVIDPVANSAGADLGHYLIVVDGIE
                     LGLRIELGETALSFGRVRPCDVVFQDEEVSRRHCTIGVLLGQVVVTDLKSTNGTFIDG
                     KPIDGVATVPVGSVVQIGRQLLKYERRSRKEVQAADELNRDLDRAGRYVRSLLPAPLT
                     GGPITTDWIYLPSARLGGDVFGYHAFDARTFAVYLVDVSGHGAESAMHAVGVMNVLRQ
                     RALPDTDFKRPAAVIQRLNAMFPMEEHASMFFTIWYGVFDAQRRVIDYCSAGHHPAYL
                     LSEDRAQITALRTPNMAVGVMPDTDFAAGAAEVPAGATLYVFSDGVFEIVTRTGTRWE
                     IDDFLPLVSAPPVPGVGEPKRLHARVSEVARPGGFDDDFSLLTVTFA"
     gene            1222..1689
                     /locus_tag="AC16HB_07145"
     CDS             1222..1689
                     /locus_tag="AC16HB_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606604.1"
                     /translation="MASVTAIEHVAKLENLPRFMAFIDAACQRAGADEETAYALRLAV
                     EEVCVNLIHYGYKDMAPGPIRVSFQQRGCRITVTIRDRARPFDPADAPEPDVTSDAET
                     RPIGGLGWYLVKRSVDRFDYRANPVRGNVLTLTKHMQQPSRQHVEGESSGTVS"
     gene            1673..2011
                     /locus_tag="AC16HB_07150"
     CDS             1673..2011
                     /locus_tag="AC16HB_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5606605.1"
                     /translation="MELSVKPAGNATVVEIRGSVDGLTADKLLQTLTEQVQGGNVRLV
                     ADCAGLDYTSSAGLRALLGTVKLARQKGGDLRLAAVRAPVLKVLELSGFTSILKHFPD
                     VKEATASFGG"
     gene            2017..2898
                     /locus_tag="AC16HB_07155"
     CDS             2017..2898
                     /locus_tag="AC16HB_07155"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5606606.1"
                     /translation="MPDSDSSRRKSRRRVAVVIGSGSVKCAAALGLINILQRERIEID
                     MLVGCSAGCLYATLIASGRSVAEATELTRQLWTSEITARRDRRSLLRALLPGVLGFDA
                     TFGLRDDTLVMQRLKSAFGDARIENMRIPLHLAATDFANGEQAVFSRGPVTDAIRASM
                     ALPFMFSPWRIGERLYIDGYMSDPLPVGVAIKEGADIIVAMGFESPYQEHINSPGRFA
                     FQVSSILSNNLLKSQFSFHGLAHHAEVIPILPEFRQRIRLFDTDKIPAIIEDGERAAE
                     QQLPYLLRLLEAEERSG"
     gene            3008..4795
                     /locus_tag="AC16HB_07160"
     CDS             3008..4795
                     /locus_tag="AC16HB_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5606607.1"
                     /translation="MERDEGSLAKSVLPLAGSIDGDATQSERTVVSHPVGVGHSLPGH
                     TLQSASAQGDDVPARQVGRYQIIAKLGEGAMATVYRAFDPSIGRALVIKFLHADLCVD
                     AEYRNRFLREAKAAGALSHANIVTVFDVGEIEGRPYIAMELVDGGALSDQLKKGEGLA
                     LRDVVRLGLQVAHALDYAHSHGIFHRDIKPSNLLLLKDGTTVKVADFGIAHMAGQDAG
                     DRTRVGTVIGTPHYMSPEQAMGAKIDGRSDLFSLGVVLYQLVTGTRPFEADSMVSLAH
                     RIAREEPRPIDELRADVPPALRRIVERCLRKQPDKRFQTGREVADALAKVWRDMEEEA
                     DGRGRPRRIPLKLKLALGMAALVAATMAVTSAFVTQRQYQTMLAQTLDHGASLTKMIA
                     VESAASALSEDWVGIDVFVQEVARSLALKSLAVSDGRGLVHVSADAAQVGKQAAPLAG
                     EALPSEERGVRVVRTALRSGESVFDFEAPITFQTKRVGTVRLALSEAPVVRATRQSLL
                     LLGLLLVITAATVGLATYVLVEHYAKPLRLLRESLDEIRNGRYGYRISEKRADEFGEV
                     YTAFDAMAARLERDAPGSGESAPPSGKPA"
     gene            4795..5835
                     /locus_tag="AC16HB_07165"
     CDS             4795..5835
                     /locus_tag="AC16HB_07165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5606608.1"
                     /translation="MTALRASSARLLACAAAALVVACATPPDASHRPAPAATPEVRSE
                     SVIARDDRFVIYAPEDGDTLASLARRFLGTEERAWEIADFNGITRIERGLVVVIPLRP
                     VNPKGVTASGYQTVPILTYHRVGPRVNRMIVSPDAFEAQLEYLKRNDYRVIRLADLPD
                     FLEGRKQMPKRAVAITFDDGHVSSYQYAFPLLKKYGFHATYYLYTDFLGARDALTWAQ
                     IREMHESGLIDFEAHSKTHSNLIVRNADESEQRYRDRLDLEIRQPRDLIQRHLPTRVT
                     EFAYPYGDANEAVLDRLTQAGNYRLGLTVNPGGNAFFAHPFMLRRTMVFGEHDMAAFK
                     AALQVFREINLK"
     gene            5832..6410
                     /locus_tag="AC16HB_07170"
     CDS             5832..6410
                     /locus_tag="AC16HB_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606609.1"
                     /translation="MIGRLATVGGLAALLLAGCVTPPARVSEPTAAPAALPARGQTLE
                     RDYRARATVLMREQRWAEAKVQLELLLLLHPGAIEYRTQLDSVRRNIAELAAEANATA
                     ATARKRGDLDTATAQYLRALAADRDNAVAAQGLREIERERARRAWLNRPPRSMLGVMT
                     GKPDAPAELEPEYMQRSRPGNGNGNETAAPRR"
     gene            complement(6429..7229)
                     /locus_tag="AC16HB_07175"
     CDS             complement(6429..7229)
                     /locus_tag="AC16HB_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5606610.1"
                     /translation="MRLPLILFVACASIAACQTPSDSSRSTASSDAKAATPAPSPPSE
                     PKVEGPAPGSAAAKSQAQVLLKQAAEQLNEGNEDVAREEIAQALTLDPGNKTGVCLQR
                     GLTADPVATLGRDSTSYTVRSGDTLGSIAQRALGESCEFYLLARYNLIRVPSRLNVGQ
                     VLRIPGKVPLTAPERTAKPGETPAPASDPAAPPSHFGSQRGAMKAAEVKALIERHQRN
                     AIAAFRKQDLATSIKEWDRVLELDPGNDLARARRQEAIELQRRLNQVK"
     gene            7710..8111
                     /locus_tag="AC16HB_07180"
     CDS             7710..8111
                     /locus_tag="AC16HB_07180"
                     /EC_number="4.6.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046589 - ribonuclease T1 activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanyl-specific ribonuclease"
                     /protein_id="HIR5606611.1"
                     /translation="MKSRRPDPTRPLAHLTLLLAALLLCAGTAFARGAIGEIAAAELP
                     LEARNTLALIQRGGPFPFRKDGTVFQNRERRLPLRPRGYYTEYTVPTRGARDRGARRI
                     IAGRGGTGSPANSGEYWYTDDHYETFSRIRE"
     gene            8122..>8384
                     /locus_tag="AC16HB_07185"
     CDS             8122..>8384
                     /locus_tag="AC16HB_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HIR5606612.1"
                     /translation="MNKRPPTSLADLPAHIVAARSTLDVDQLRRWAEEAGQRFVLADL
                     SACDDRRAVLREVGRAFAFPEWYGANLDALYDCLTDLAEPGATG"
BASE COUNT         1331 a         2800 c         2888 g         1365 t
ORIGIN      
        1 ggccttgcgc acgtgcgcgg atactcctaa tctcgccgtt ctttgcattg ccgccaagat
       61 cgatccgtga gcgagattcc aggtgggacc accggttggg acggccgcac gacgctgctc
      121 gaccgcgtga tcgatccggt cgcgaactcg gccggagccg atctgggtca ctacctgatc
      181 gtcgtcgacg gcatcgagct gggcctgcgc atcgagctcg gcgagacggc gctctccttc
      241 ggtcgcgttc gtccgtgtga cgtcgtgttc caggacgagg aagtctcgcg ccgccattgc
      301 acgatcggtg ttctgctggg gcaggtcgtc gtcacggacc tgaagtcgac caacggcacg
      361 ttcatcgacg gcaaacccat cgacggggtg gcgacggtgc cggtcggctc cgtggtgcag
      421 atcggccgcc agttgctgaa gtacgagcgt cgcagccgca aggaagtgca ggccgcggac
      481 gaactgaatc gcgacctcga tcgcgccggc cgctacgtcc gctcgctgct gcccgcgccg
      541 ctgaccggcg ggccgatcac caccgactgg atctatctgc catcggcgcg gctcggcggc
      601 gacgtgttcg gctaccacgc gttcgatgcg cgcacgttcg cggtgtatct ggtcgacgtt
      661 tcgggccacg gcgctgaatc cgcgatgcac gccgtcggag tgatgaatgt gctgcggcag
      721 cgtgcgctac ccgacaccga tttcaagcgc cccgccgcgg tgatccagcg gctgaacgcg
      781 atgttcccga tggaggagca cgcgtcgatg ttcttcacga tctggtatgg cgtgttcgac
      841 gcgcagcggc gcgtgatcga ctactgctcg gccgggcacc atcccgcgta cctgctgtcg
      901 gaggaccggg cgcagataac cgcgctccgc acgccgaaca tggcggtggg cgtgatgccg
      961 gacaccgact tcgccgcagg ggccgcagaa gtgccggcgg gcgcgacgct ttatgttttc
     1021 agcgacggcg tgttcgagat cgtcacgaga accggcacgc ggtgggagat cgatgatttc
     1081 ctgccgcttg tcagcgcgcc cccggtgcct ggggtcgggg agcccaaacg gttacacgcg
     1141 cgcgtgtccg aagtcgcgcg gccgggcggc ttcgacgatg atttctcgct gctcacggtc
     1201 accttcgctt gagtccgcct catggccagt gttacggcga tcgagcacgt cgcgaagctg
     1261 gagaatctgc cgcgcttcat ggcgttcatc gacgcggcct gccagcgtgc cggcgccgac
     1321 gaggagaccg cgtacgcgct gcgactggca gtggaagagg tgtgcgtcaa cctgattcat
     1381 tatggctaca aggacatggc gccgggcccg atccgagttt cgtttcagca gcgcggctgc
     1441 cggatcacgg tgacgatccg cgaccgcgcg cgcccgttcg atccggccga tgcccccgag
     1501 ccggacgtga cctcggatgc ggagactcgc ccgatcggcg gtctcggctg gtatctcgtc
     1561 aagcgatcgg tcgatcgctt cgactaccgc gccaatccgg tgcgcggcaa cgttttgacc
     1621 ttgaccaagc acatgcagca accatcgcgc cagcacgtgg aaggagaatc cagtggaact
     1681 gtcagttaag ccagcaggga acgcgacggt cgtcgaaatc cgtggcagcg tcgacggcct
     1741 gaccgcggac aaactgctgc aaacgctgac cgagcaagta caaggcggaa acgtccggct
     1801 ggtcgccgac tgtgctggtc tggactacac cagcagcgcg gggctgcgcg cgttgctcgg
     1861 caccgtcaag ctggcgcgcc agaagggcgg cgacctgcgc ttggccgcgg tgcgcgcgcc
     1921 ggtcctcaaa gtgctcgaac tgtccggctt cacgtcgatc ctgaagcact ttccggacgt
     1981 gaaggaagcg accgcaagct tcggcggctg acgatggtgc cggattccga cagttcgcgg
     2041 cgcaagtcgc gccggcgcgt cgcggtagtg attggctcgg gcagcgtcaa gtgcgccgcg
     2101 gcgctcggac tgatcaacat actgcagcgt gaacgcatcg agatcgacat gctggtcggg
     2161 tgcagcgccg gctgcctgta tgcgacgctg atcgcatcgg gccgcagcgt ggccgaggcg
     2221 accgagctga cgcgccagct gtggacgagc gaaatcaccg cgcgacgcga ccgccgttcg
     2281 ctgctgcgcg cgctgctgcc gggcgtgctc ggcttcgatg ccacgttcgg gttgcgcgac
     2341 gacacgctgg tgatgcagcg cctgaagagc gcgttcggcg atgcgcgcat cgagaacatg
     2401 cggattccgc tgcacctggc ggcaacggat ttcgccaatg gcgaacaggc cgtgttctcc
     2461 cgcgggccgg tgaccgatgc gatccgcgcc agcatggcgc tgccgttcat gttcagcccc
     2521 tggcgcatcg gcgagcggct gtatatcgac ggctacatgt cggatccgct gccggtcggc
     2581 gtggcgatca aggagggcgc cgacatcatc gtcgcgatgg gcttcgaaag cccataccag
     2641 gagcacatca actcgccggg gcgcttcgca ttccaggtca gctcgatcct gagcaacaac
     2701 ctgctcaagt cgcagttctc cttccatggc ctcgcgcacc acgccgaggt gattccgatc
     2761 ctgcccgaat tccggcaacg catccgcttg ttcgacaccg acaagatccc cgccatcatc
     2821 gaggacggcg agcgcgccgc cgagcagcag ctgccgtacc tgctgcggct gctggaggcg
     2881 gaggaacgca gcggctgagc cgttcggctg atcgtccggg gcgtgcccgt acgcgccgga
     2941 ctcgaatacc atccgctccg gatctgggcg cgtcgccgcg cgagcggggc ccgtgcagga
     3001 gaacgacgtg gagagggacg agggatcact ggcgaaatcg gtgctgccgc tggccggcag
     3061 catcgacggc gatgcgacgc agagcgaacg caccgtcgtc tcgcatcccg tcggtgtcgg
     3121 gcattccttg ccggggcaca cgctgcagtc agccagcgcg cagggcgacg acgttccagc
     3181 gcgacaggtc ggccgctatc agatcatcgc caagctcggc gaaggcgcga tggccacggt
     3241 gtacagggcg ttcgatccca gcatcggccg cgcgctggtc atcaagttcc tgcacgccga
     3301 cctgtgcgtc gatgcggagt accgcaatcg cttcctgcgc gaggccaagg cggcgggtgc
     3361 gctgtcgcac gcgaacatcg tcaccgtgtt cgatgtcggc gagatcgagg gacggccgta
     3421 cattgcgatg gagctggtcg acggcggcgc gctgtcggat cagctcaaga agggtgaggg
     3481 gctcgcactg cgcgacgtcg tgcggctcgg cctgcaggtc gcgcatgcgc tcgactacgc
     3541 gcacagtcat ggcatctttc atcgcgacat caagccgagc aacctgctgc tgctgaagga
     3601 cggcacgacc gtgaaggtgg ccgacttcgg catcgcgcac atggcagggc aggatgccgg
     3661 cgaccgcacg cgtgtcggca cggtgatcgg tacgccgcac tacatgtcgc ccgagcaggc
     3721 gatgggcgcg aagatcgacg gccggtcgga cctgttctcg ctcggcgtcg tgctgtatca
     3781 gctcgtcacc ggcacgcggc cgttcgaagc cgacagcatg gtgtcgctcg cgcaccggat
     3841 cgcgcgagag gagccgcgac cgatcgatga actgcgcgcc gacgtgccgc cggcgctgcg
     3901 gcggatcgtc gagcgctgcc tgcgcaagca gcccgacaag cgcttccaga ccggtcgcga
     3961 ggtggccgac gcgctggcca aggtctggcg cgacatggag gaggaggccg acgggcgcgg
     4021 ccggccgcgg cggattccgc tgaagctgaa gctcgcgctc ggaatggcgg cgctggtcgc
     4081 ggcgacgatg gcggtcacgt cggcgttcgt cacacagcgg cagtaccaga caatgctggc
     4141 gcagacgctc gatcacggcg cgtcgctgac caagatgatc gcggtcgaga gcgcggcctc
     4201 tgcgctgtcg gaggactggg tcggcatcga cgtgttcgtg caggaagtcg cgcgttcgct
     4261 ggcgttgaag tcgctggcgg tcagcgacgg ccgcgggctt gtgcacgtca gcgcggatgc
     4321 cgcgcaggtc ggcaagcagg ccgcgccgct ggcgggtgag gcgctgccga gcgaggagcg
     4381 cggcgtgcgc gtggtgcgca ccgcgctgcg ctccggggaa tcggtgttcg acttcgaggc
     4441 gccgatcacc tttcagacca agcgcgtcgg cacggtgcgg ctcgcgctat cggaggcgcc
     4501 ggtcgtccgc gcgacgcgtc agagcctgct gttgctcggg ctgcttctcg tgatcaccgc
     4561 agcgaccgtc gggctcgcca cgtacgtgct ggtcgagcat tacgccaagc cgctgcgact
     4621 gctgcgcgag tcgctggacg agatacgcaa cggccgctac ggctatcgca tctcggagaa
     4681 gcgcgcggac gaattcggcg aggtgtacac ggcgttcgac gcgatggccg cgcggctcga
     4741 gcgcgatgca cccggatcgg gcgaatcggc gcccccgtcc gggaagccgg cgtgatgact
     4801 gcactgcgcg cgtcatctgc acggttgctg gcttgcgccg cggccgcgct ggtcgtggct
     4861 tgcgccacgc cacccgacgc ctcgcaccga cctgcgccgg ccgccacgcc ggaagtgcgg
     4921 tccgaatcgg tcatcgcgcg cgacgaccga ttcgtgatct acgcgccgga ggacggcgac
     4981 acgctcgcgt cgctggcgcg gcgcttcctc ggcaccgagg aacgcgcgtg ggagatcgct
     5041 gatttcaacg gcatcacgcg catcgagcgc ggcctcgtcg tcgtcattcc actgcgaccg
     5101 gtcaacccga agggcgtgac tgcatccggc taccagacgg tgccgatcct gacctaccac
     5161 cgcgtcgggc cgcgcgtgaa ccggatgatc gtctcgcccg acgcattcga ggcccagctc
     5221 gagtacctca agcgcaacga ctaccgggtg attcgcctcg cggacctacc cgattttctc
     5281 gaaggtcgga agcagatgcc gaagcgcgct gtcgcgatca cgttcgacga cggccacgtt
     5341 tcctcgtacc agtatgcgtt cccactgctg aagaagtacg gattccacgc gacctactac
     5401 ctgtacacgg acttcctcgg cgcgcgcgac gccctgacgt gggcgcagat tcgcgaaatg
     5461 cacgagtcgg gcctgatcga cttcgaggcg cactcgaaga cgcattcgaa tttgatcgtt
     5521 cgcaacgccg acgaatccga gcagcgctat cgcgatcgcc tcgatctgga gatccgccag
     5581 ccgcgcgacc tgatccagcg ccatctgccg acgcgcgtga cggaattcgc ctatccctac
     5641 ggcgacgcaa acgaggccgt gctcgaccgg ctcacgcagg cgggcaacta caggctcggc
     5701 ctgacggtca atccgggcgg caatgccttc ttcgcgcatc cgttcatgct gcggcggacg
     5761 atggtgttcg gcgagcacga catggcggcc ttcaaggcgg cgctgcaggt ctttcgcgag
     5821 atcaacctga agtgatcggt cggctcgcga cagtcggtgg gctcgccgcg ctcttgctgg
     5881 cgggttgtgt cacgccgccg gcgcgggtct ccgagcccac ggcggctccg gcggcactgc
     5941 cggcgcgcgg acaaacgctc gagcgcgact accgggcgcg cgcgacggtg ttgatgcggg
     6001 agcagcgctg ggccgaggcg aaagtgcagc tggaactgct gctgctgctg cacccgggcg
     6061 ccatcgagta ccgcacccag ctcgactcgg tacgcaggaa catcgcggaa cttgctgccg
     6121 aggccaatgc aacggcggcg accgcgcgca agcgcggcga tctcgacacg gcgaccgccc
     6181 agtacctgcg cgcgctggcg gccgatcgcg acaatgcagt ggccgctcag ggactgcgcg
     6241 agatcgaacg cgagcgcgcg cgtcgcgcct ggctgaaccg cccgccgcgt tcgatgctcg
     6301 gcgtgatgac gggaaagccc gacgcgccgg ccgagctcga accggagtac atgcagcggt
     6361 cgaggcccgg aaacggcaat ggcaacgaaa ccgccgcgcc gcgccgctga ccgcctgctc
     6421 cgccgcagct acttgacctg attcaatcgg cgctgcagtt cgatcgcttc ctggcggcgg
     6481 gcccttgcga ggtcgttgcc gggatcaagc tcgagcacgc ggtcccattc cttgatcgaa
     6541 gtcgcgaggt cctgcttgcg aaacgccgcg attgcgtttc tctggtgacg ttcgatgagc
     6601 gccttgacct cggcggcctt catcgcgccg cgctgggaac cgaagtgcga cggtggtgct
     6661 gccggatcgc tcgcgggtgc aggcgtctcc cccggcttgg ccgtgcgctc gggtgccgtc
     6721 agcggcactt tgcccgggat tcgcaacacc tgtcccacgt tcagccggct cggcacccgg
     6781 atcaggttgt agcgcgccag cagatagaac tcgcagctct cgcccagcgc ccgctgcgcg
     6841 atcgaaccca gcgtatcccc cgatcgaacc gtgtagctgg tcgaatcgcg gcccagtgtc
     6901 gcgacgggat ccgcagtcag cccccgctgc aggcacacgc ccgtcttgtt gcccggatcc
     6961 agggtgaggg cctgcgcgat ctcctcgcgc gcgacgtcct cgttgccttc gttcaactgc
     7021 tccgccgcct gcttcagcag gacctgcgcc tggcttttgg cggcggcgga gccgggtgcc
     7081 ggtccttcga ctttgggctc ggacggcggg gacggcgccg gcgtggccgc cttcgcgtcc
     7141 gacgaagcgg tcgaccggga cgaatcggaa ggcgtctggc aggccgcgat cgaagcgcag
     7201 gcgacgaaca ggatgagggg aagtcgcatc gtttcagcgc gcgccgcagc cggccgcgtc
     7261 gatgcgagca tcataagcgc cacgtcgcgg cagcggaacg gccggcaccg gattgccggc
     7321 gcgaatgctc actctgaatc gcggatgcaa cgttcgagcg cgacggccgt ccggcgcggc
     7381 gctctcatcc agccacaaag caagaatgac cggcgcacgc cgcctcatgc ggctgcggac
     7441 cgctccaccc attcccgatc ccggtcgatt gcgcggcggg gtagctcggt gcccccggcc
     7501 cgccgagcga ggttacgagg tccgcccatc cgaagctcgc gcggctcgcc ggcgacgcgc
     7561 tgcgttggcg ccgcgtcgat ttgccgtcga ttgcgccgtg ctcgcccgtc agcagcggct
     7621 cgaacgtgac gacacgcggc gattgatcga acctggttgc gccgatacgc tggcgcgatg
     7681 cgacgtgctt tgcgtatccc cgtgccagga tgaaatcgcg gcgacccgat ccgacgcggc
     7741 cgcttgcgca cttgacgctg ctgctggcgg cactgctgct atgcgcgggc acggccttcg
     7801 cgcgcggcgc aatcggcgaa attgcggccg ccgaacttcc gctcgaggcg cgcaacacgc
     7861 tggcgctgat ccagcgcggc ggcccgttcc cgtttcgcaa ggacggcacg gtgtttcaga
     7921 atcgcgaacg ccggttgccg ctgcggccac gcggttacta caccgagtac acggtgccga
     7981 cacggggcgc gcgcgatcgc ggtgcgcgcc ggatcatcgc gggtcgaggg ggcaccggca
     8041 gccccgcgaa cagcggtgaa tactggtaca cggacgatca ctacgagacg tttagccgca
     8101 ttcgtgaatg acgcgccgcc gatgaacaag cgcccgccca cctcgctcgc cgatctgcct
     8161 gcgcacatcg ttgcggctcg cagcacgctc gacgtcgatc aattgcggcg ctgggccgag
     8221 gaagccggac agcgtttcgt gctggccgat ctttcggcct gcgacgatcg tcgagcggtg
     8281 ctgcgcgagg ttggccgcgc gtttgccttt cccgagtggt acggtgccaa tctcgacgcg
     8341 ctgtacgact gcctgacgga tctggccgag cccggcgcga ccgg
//
LOCUS       DBNHBY010000292         4604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1371090, whole genome shotgun sequence.
ACCESSION   DBNHBY010000292 DBNHBY010000000
VERSION     DBNHBY010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1371090"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1008
                     /locus_tag="AC16HB_07190"
     CDS             151..1008
                     /locus_tag="AC16HB_07190"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5606613.1"
                     /translation="MDWLLAIKATILGIVEGLTEFLPISSTGHLIVAGSLLDFTGETA
                     KTFEIAIQTGAMLAVIWEYRQRIAATVSGVFGDATAQRFALNVLIAFIPAVVLGLAFS
                     RIIKAYLFAPVPVAIAFIVGAFVILWVEARHKRRYGARDLEGQRHARVETVDAMTPLD
                     ALMVGLAQCCALVPGTSRSGATIIGGMIFGLSRKAATEFSFYLAIPTLIGAGVYSLWK
                     QRASLSSADLPLFAIGAVFAFVSALFCIRWLIRYISTHDFRLFAWYRIAFGAVILITA
                     WTGVVSWAE"
     gene            1013..1642
                     /locus_tag="AC16HB_07195"
     CDS             1013..1642
                     /locus_tag="AC16HB_07195"
                     /inference="COORDINATES: protein motif:HMM:NF040120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HIR5606614.1"
                     /translation="MSGERRLELVAAIASAAIVAAGVLFFGWPTFTVLAFYWTENVAI
                     GAFNLLRILVAAARARQAGSGVFLAGFFVVHYGLFCLVHAYFIVALFGGGTDDRGLFD
                     SSAALLARVASDSIGVAALAAIVVAAGTDALRWIANGAAADRADPNAALFAPYGRIVV
                     LHVVLLGGGFLLQLVRAPAIAALLLVGAKLVSDWLRLRGTPDAATATAD"
     gene            1647..2177
                     /locus_tag="AC16HB_07200"
     CDS             1647..2177
                     /locus_tag="AC16HB_07200"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR5606615.1"
                     /translation="MERRIGNACIEVLETDITSLAVDAIVNAANEMLAPGGGVCGAIH
                     RAAGPELARECAQLGGCATGDAKITRGYRLPARHVIHAVGPVWHGGGKGEPAHLRSCY
                     VRALELARQHGLRSIAFPAISTGIFGYPIDAAAQVAVSAVFDTLPLAPTVERVVFACF
                     GAPNRLALEAALRALR"
     gene            complement(2184..3200)
                     /locus_tag="AC16HB_07205"
     CDS             complement(2184..3200)
                     /locus_tag="AC16HB_07205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5606616.1"
                     /translation="MRAWWALVAALLAFAADAASPEQLFARLRSAVVSVQVNEYVVQG
                     AERLQVMVVPSKGAGVVFTGDGKVLTAAHLVQTADEIVVEFADGTRTPAHVYASEPAA
                     DVALLQLDEQPAVSPVPRADSDAVRIGQSVVVIGAPFGLAGMQSVGYVSGRHRPATPF
                     GEMDQLEMFVIDAHVEQGSSGGGVFDDAGRLIGMVTSVQFAAGRSFGLGFVITSNTID
                     ALLVDRRSFWSGFGGYLLAGKLAQVLNVPQNCGLLVQRVARSSPAAQIGLRGGTMIAS
                     VGDDRFVIGGDIVLGMQGIALDGSKEYARIQRELAQFADGDTLRVTVLRGGRILELEG
                     PVTR"
     gene            complement(3340..4047)
                     /gene="trmB"
                     /locus_tag="AC16HB_07210"
     CDS             complement(3340..4047)
                     /gene="trmB"
                     /locus_tag="AC16HB_07210"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR5606617.1"
                     /translation="MSDTAEIAGPRYTHIRSFALRRGHVTPAQRRAYEEIFPRYAVPY
                     TNTPLDYARVFGRAAPTVLEIGFGMGETTAVIAQARPDVNFLCVEVFVAGIGALAKRI
                     EAMELANIRIVQHDAVEVVRDMLAADSLAGVHVFFPDPWPKARHHKRRLIAQPFVGQM
                     AGRIARGGYLHAATDWQHYAEQMLDVLSNEPQLRNLHDGFAPSSRNPLTERPTTKFHA
                     RGEKLGHGVWDLVFERC"
     gene            complement(4047..>4604)
                     /locus_tag="AC16HB_07215"
     CDS             complement(4047..>4604)
                     /locus_tag="AC16HB_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR5606618.1"
                     /translation="AEDAVRTLRLARELLDGHALTKLEVLGDPQTLYPNMPETIKAAE
                     ILVKEGFEVMVYCSDDPIQAQALESIGCVAVMPLASLIGSGMGILNPWNIQLILERAK
                     VPVIVDAGVGTASDAAIAMELGCAGVLMNTAVAQAQDPVLMASAMKKAVQAGREAFRA
                     GRMPRKFYSASPSSPTAGVIASTRS"
BASE COUNT          657 a         1624 c         1573 g          750 t
ORIGIN      
        1 atggcgctgc ccgccgagcg tctgcgccag gatcaggtcg cgtacggcgc cacgggcgtc
       61 agcgtcggct acacggggct gaagatcgac atcgcaccga agtgactcgc gctccccgat
      121 aatgcgcgct ttgcctacac ggagcgcgca ttggactggt tgctggcaat caaggcgacg
      181 atcctcggca tcgtcgaggg gctgaccgag ttcctgccga tctcgagcac cggccacctg
      241 atcgtcgccg gatcgctgct ggatttcacg ggcgagaccg ccaagacatt cgagatcgcg
      301 atccagaccg gcgcgatgct ggccgtgatc tgggagtacc ggcagcggat cgcggcgacc
      361 gtgtccggcg tgttcggcga tgcgaccgcg cagcgcttcg cgctcaacgt gctgatcgcg
      421 ttcatccccg ccgtggtgct cggactagcg ttcagccgga tcatcaaggc gtacctgttc
      481 gcgccggtcc cggtcgcaat cgcgttcatc gtcggcgcgt tcgtgatcct gtgggtcgag
      541 gcgcgccaca agcgacgcta cggtgcgcgc gacctcgaag ggcagcgcca tgcgcgcgtg
      601 gaaaccgtcg acgcgatgac gccgctcgac gcgctgatgg tgggcctggc gcaatgctgc
      661 gcgctggtgc cggggacgag ccgctcgggc gcgaccatca tcggcggcat gatcttcggt
      721 ctgtcgcgca aagcagcgac cgaattcagt ttctatctgg cgattccgac gctgatcggc
      781 gccggcgtct actcgctgtg gaagcagcgc gcatcgctca gcagcgccga cttgccgctg
      841 ttcgcaatcg gcgcggtgtt cgcgttcgtg agtgccctgt tctgcatccg ctggctgatc
      901 cgctacatct ccacccacga ctttcgcctg ttcgcgtggt accggatcgc cttcggcgcg
      961 gtgatactga tcacggcctg gaccggcgtg gtgagctggg ccgaatgatg cgatgagcgg
     1021 cgagcgacgg ctcgaactcg tggcggcgat cgcctccgcc gcgatcgtcg ccgcgggcgt
     1081 gctgttcttc ggctggccga cgttcaccgt gctggctttc tactggaccg agaacgtggc
     1141 gatcggcgct ttcaacctgc tgcgcatcct cgtcgccgcg gcgcgcgcgc gacaggccgg
     1201 cagcggcgtg ttcctcgccg gtttcttcgt cgttcactac gggctgttct gcctcgtgca
     1261 cgcctacttc atcgtcgcgc tcttcggggg tggcaccgac gaccgcggct tgttcgattc
     1321 gtccgccgcg ctgctggcgc gagtcgcttc cgattcaatc ggcgtggcgg cgctcgcggc
     1381 gatcgtagtc gcggccggaa ccgacgcgct gcgctggatc gcgaacggcg ccgccgccga
     1441 tcgtgccgac ccgaacgccg cgctgttcgc gccgtatggc cgcatcgtcg tgctgcacgt
     1501 ggtgctgctg ggtggcggct tcctgttgca actggtgcgg gcgccggcga tcgcggcgct
     1561 gctgctggtg ggcgcgaagc tcgtgtccga ctggttgcgg ctgcgcggca cgccggacgc
     1621 ggcaacggcc acggcggact gacgcgatgg agcgccggat cggcaacgca tgcatcgaag
     1681 ttctcgagac cgacatcacg tcgctcgctg tcgacgcgat cgtcaacgcg gccaacgaga
     1741 tgctcgcgcc gggcggcggc gtgtgtggcg cgatccatcg cgcggcgggg ccggaactgg
     1801 cgcgcgagtg cgcgcaactc ggcggctgcg ccaccggaga cgcgaagatc acgcgcggct
     1861 atcgcctgcc ggcgcggcac gtgatccacg cggtcggccc cgtgtggcac ggcggaggga
     1921 agggcgagcc tgcgcacctg cgctcctgct atgtgcgcgc gctcgaactc gcgcggcagc
     1981 acggattgcg ctcgatcgcg tttcccgcca tctcgaccgg catcttcggc tacccgatcg
     2041 acgccgccgc gcaggtcgct gtcagtgcgg tcttcgacac gctgccgctc gcgcccacgg
     2101 tcgagcgcgt ggtgtttgcc tgcttcggcg cgccgaaccg gctcgcactg gaggccgcac
     2161 tgcgcgcgct gcggtagcgg cgctcagcgc gtcaccgggc cctcgagttc caggatgcgg
     2221 ccgccgcgca gcacggtcac gcgcagcgtg tccccgtcgg cgaactgcgc cagttcgcgc
     2281 tggatgcgcg cgtactcctt cgagccgtcg agcgcgatgc cctgcatacc cagcacgatg
     2341 tcgccaccga tgacgaagcg gtcgtcgccc acggacgcga tcatcgtgcc gccgcgcagg
     2401 ccgatctgcg ccgccggcga actgcgcgcc acgcgctgca cgagcagccc gcagttctgc
     2461 ggcacgttca gcacctgggc cagcttgccg gccagcagat agccgccgaa gccgctccag
     2521 aacgagcgcc ggtcgaccag cagcgcgtcg atcgtgttcg acgtgatcac gaagccgagc
     2581 ccgaagctgc gcccggccgc gaactgcacg ctggtgacca tgccgatcag gcggcccgcg
     2641 tcgtcgaaca cgccgccacc ggacgaaccc tgctcgacgt gcgcgtcgat cacgaacatc
     2701 tcgagctggt ccatctcgcc gaacggcgtg gccgggcggt gccggccgct gacataaccg
     2761 accgactgca tgccggccaa cccgaacggc gcgccgatca cgaccacgct ctggccgatg
     2821 cgcaccgcgt ccgagtcggc ccgggggacc ggcgacaccg caggctgctc gtcgagctgc
     2881 agcagcgcga cgtcggccgc cggctccgat gcatacacgt gcgccggcgt gcgcgtgccg
     2941 tccgcgaatt caacgacgat ctcgtcggcg gtctgcacca ggtgtgccgc ggtcagcacc
     3001 ttgccgtcgc cagtgaaaac gacgcccgcc cccttgctgg gcacgaccat cacctgcaag
     3061 cgctccgcgc cctggacgac gtattcgttg acctgcacgc tcaccacggc ggagcgcaaa
     3121 cgcgcgaaca actgctcggg cgacgccgca tcggcggcga acgcgagcaa tgccgccacg
     3181 agcgcccacc aggcgcgcat gccaggcagc gtccgacgtg atgtgtttcg cgtctgcatg
     3241 tgtgcgcggg cgaccgatcc gcaacccgat ttcatgatcg gcgcgcgctc cagtgcgtgg
     3301 ttgatctgcc gcaacgcggg tacgcgcgcg acctgacgct cagcagcgct cgaatacgag
     3361 gtcccacacg ccgtggccga gcttctcgcc gcgcgcgtga aacttggtgg tcggccgttc
     3421 cgtgagcgga ttgcgcgacg atggtgcgaa gccgtcgtgc aggttgcgca actgcggctc
     3481 gttcgacagc acgtcgagca tctgctcggc atagtgctgc cagtcggtgg cggcgtgcag
     3541 gtagccgccg cgcgcgatcc ggcccgccat ctggccgacg aagggctgcg cgatcaggcg
     3601 ccgcttgtga tgccgcgcct tcggccacgg gtccgggaag aacacgtgca cgccggcgag
     3661 cgaatccgct gcgagcatgt cgcgcaccac ctcgaccgcg tcgtgctgga cgatgcggat
     3721 gtttgccagc tccatcgctt cgatccgctt cgcgagcgcg ccgatgccgg cgacgaacac
     3781 ctcgacgcag aggaagttca cgtccggccg cgcctgcgcg atcacggccg tggtctcgcc
     3841 catgccgaag ccgatctcga gcacggtcgg cgcagcgcgg ccgaacacgc gcgcgtagtc
     3901 gagtggcgtg ttcgtgtacg gcaccgcgta gcgcgggaag atctcctcgt aggcgcgccg
     3961 ctgcgccggc gtgacgtggc cgcgccggag cgcgaacgag cggatgtgcg tgtagcgcgg
     4021 tcccgcgatc tccgcggtat cgctcatcac gagcgggtcg atgcgatcac gcccgcggtc
     4081 ggcgagctcg ggctcgcact gtagaacttc ctcggcatgc ggccggcgcg gaacgcctcg
     4141 cggccggcct ggaccgcctt cttcatcgcg ctcgccatca gcaccgggtc ctgcgcctgc
     4201 gccaccgcgg tgttcatcag cacgccggcg cagccgagtt ccattgcgat cgcggcatcc
     4261 gacgccgtgc cgacgccggc gtcgacgatc accggcacct tggcgcgctc gaggatcagc
     4321 tggatgttcc acgggttcag gatgcccatg cccgagccga tcagcgacgc gagcggcatc
     4381 accgccacgc acccgatcga ttcgagcgcc tgcgcctgga tcgggtcatc cgagcagtac
     4441 accatgacct cgaagccttc cttcaccagg atctcggcgg ccttgatcgt ctccggcatg
     4501 ttgggataca gcgtctgcgg atcgccgagc acctcgagct tggtgagcgc gtggccgtcg
     4561 agcagctcgc gcgccaggcg cagcgtgcgc accgcgtcct cggc
//
LOCUS       DBNHBY010000293         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1776502, whole genome shotgun sequence.
ACCESSION   DBNHBY010000293 DBNHBY010000000
VERSION     DBNHBY010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1776502"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16HB_07220"
     CDS             complement(<1..877)
                     /locus_tag="AC16HB_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606619.1"
                     /translation="MRWLYAFLLAALCAVAAPSRAATELTFYYPIAVGGPLTKVIDGY
                     AREFEKSHPDIKINPIYAGNYDDARIKALAALKAGQSAPLSVLFSIDIYELLEQDAIV
                     AWDDVATSADDKAWLKAFYPALMANGTYKNKVYGIPFQRSTIVLYWNKDAFKEAGLDP
                     DKPPANWDEMTQMAAKLVKKDAAGNVTRWGVMVPSTGYAYWMFQAFARQNGHDLMNRE
                     GNATNYADPDVIAALQYWRDLGAKYKVMPEGTVEWGTLRQAFTEGKTAMMWHTTGNLT
                     AVKDSAKFPFGVAMLP"
     gene            complement(940..2010)
                     /locus_tag="AC16HB_07225"
     CDS             complement(940..2010)
                     /locus_tag="AC16HB_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606620.1"
                     /translation="MTDIVLEGVSKRYGAVRAVDDISLRADSGKFLVLLGPSGCGKST
                     VLRLIAGLEEPTAGRILIDGRDVTRLNPDKRRVSMVFQSYALFPHLSVAENIVFGLKV
                     RRTPPAARAERLARVAALVGLSEQLARKPSQLSGGQRQRVALARAIVAENKICLMDEP
                     LSNLDAQLRHGMRVEIRALQQRLGMTVVYVTHDQVEAMSMADRVILMREGRIEQEGTP
                     EELYARPTSLFAARFIGTPAMNLVALDDGPDGAVIRGAREPVLRVRGKGLTLGIRPEH
                     IRLVEADGVAAEVTSTEYHGADTILTARVGQESLLLRAPGQLRLGSGAAVRLGWDPGS
                     VHLFDAAGGARVDPGQTAGSIS"
BASE COUNT          306 a          725 c          670 g          367 t
ORIGIN      
        1 ccggcagcat cgccacgccg aacgggaatt tcgcgctgtc cttcaccgcg gtgaggttgc
       61 cggtcgtgtg ccacatcatc gcggtcttgc cctcggtgaa ggcctgccgc agcgtgcccc
      121 attcgaccgt gccctcgggc atcaccttgt acttcgcgcc cagatcgcgc cagtactgca
      181 gcgctgcgat cacgtccgga tccgcgtagt tggtcgcgtt gccttcccgg ttcatcaggt
      241 cgtggccgtt ctggcgcgcg aaggcctgga acatccagta tgcgtagccg gtcgacggca
      301 ccatcacgcc ccagcgcgtg acgttgcctg ccgcgtcttt cttgaccagc ttcgccgcca
      361 tctgcgtcat ctcgtcccag ttggcaggcg gcttgtccgg gtcgagaccg gcttccttga
      421 acgcgtcctt gttccagtac agcacgatcg tcgagcgctg gaacgggatg ccgtacacct
      481 tgttcttgta cgtgccgttg gccatcagcg ccggatagaa cgccttcagc cacgccttgt
      541 cgtcggccga cgtggccaca tcgtcccagg cgacgatcgc gtcctgctcg agcagttcgt
      601 agatgtcgat cgagaacagc accgacagcg gcgccgactg gccggctttc agcgccgcca
      661 gcgccttgat acgcgcgtcg tcgtagttgc cggcgtagat cggattgatc ttgatgtcgg
      721 ggtgcgactt ctcgaactcg cgcgcgtagc cgtcgatcac cttggtgagc ggtccgccga
      781 ccgcaatcgg gtaatagaac gtcagctcgg tcgcggcgcg cgacggtgcc gccactgcgc
      841 acagcgccgc cagcaggaac gcatacagcc atctcatggt cgacctcctt gtcggttgtg
      901 ttgctcgtct ccgggcgaag ggcattgttg caggactctt cacgaaatcg accccgccgt
      961 ttgacccgga tcaacccgcg cgccgccggc ggcgtcgaac aggtgcaccg accccgggtc
     1021 ccagccgagc cgcaccgctg cgccactgcc gagcctcagc tggccgggcg cgcgcagcag
     1081 cagtgattcc tggccgacgc gcgcggtgag gatcgtatcg gcgccgtggt actcggtgct
     1141 cgtcacctcg gcggccacgc catcggcttc gaccaggcgg atgtgctccg gccggatgcc
     1201 gagcgtgagt cccttgccgc gcacgcgcag taccggctcg cgtgcgccgc ggatcaccgc
     1261 gccatccggc ccgtcgtcga gcgcgaccag gttcatcgcc ggcgtgccga tgaagcgcgc
     1321 ggcgaacagg ctcgtcggac gcgcatacag ttcctcgggc gtgccttcct gctcgattcg
     1381 accctcgcgc atcaggatca cgcggtcggc catactcatc gcctcgactt ggtcgtgcgt
     1441 cacgtagacc accgtcatgc cgagccgctg ctgcagcgcg cggatctcca cgcgcatgcc
     1501 gtggcgcagt tgtgcgtcga ggttcgacag cggctcgtcc atcaggcaga tcttgttctc
     1561 ggcgacgatc gcgcgcgcga gcgcgacgcg ctgccgctgc ccgcccgaaa gctgagaggg
     1621 cttgcgcgcg agttgctccg acaagcccac cagcgcggcg acgcgcgcca ggcgctcggc
     1681 gcgcgctgcc ggtggcgtgc gccgcacctt caggccgaac acgatgttct cggcgaccga
     1741 caggtgcggg aacagcgcat acgactggaa caccatcgat acgcggcgct tgtcgggatt
     1801 caggcgagtc acgtcgcggc cgtcgatcag gatccggccc gcggtcggct cttcgagtcc
     1861 cgcgatcagc cgcagcacgg tcgacttgcc gcagcccgac ggtccgagca gcaccaggaa
     1921 cttgccggag tcggcgcgca ggctgatgtc atcgaccgcg cgcaccgcac cgtagcgctt
     1981 ggacactccc tcgaggacga tgtcggtcat ggcgcgcgga ttatgcaggc gaaagcggcc
     2041 gctcagtgcg cttggcgccg gtatgcgc
//
LOCUS       DBNHBY010000294         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1032227, whole genome shotgun sequence.
ACCESSION   DBNHBY010000294 DBNHBY010000000
VERSION     DBNHBY010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1032227"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="AC16HB_07230"
     CDS             complement(<1..161)
                     /locus_tag="AC16HB_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5606621.1"
                     /translation="MSQLMEVKVPDIGDFKDVEVIEVLVKAGDVVAKEQSLITVESDK
                     ASMEIPSSAA"
     gene            complement(158..751)
                     /locus_tag="AC16HB_07235"
     CDS             complement(158..751)
                     /locus_tag="AC16HB_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606622.1"
                     /translation="MTTIVVVKKNREIAIASDALVTFGETRLPHGYEVNEKIITVGDS
                     YIGLAGSTAHFAVLADALRSMGGDCRLGGRVEIFATFQRLHALLKEKYFLNPKEDESD
                     PYESSQITAVIANASGIYGVYSYREVFSFDKFWGIGSGRNFALGAMYAVYDRGRSARQ
                     IAEVGVKAGCEFDKSSALPVRVFSFPMSVAEDQERTA"
     gene            complement(755..>1924)
                     /locus_tag="AC16HB_07240"
     CDS             complement(755..>1924)
                     /locus_tag="AC16HB_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR5606623.1"
                     /translation="SSAAGTVHEMRVKLGDKVSEGSVILTLAATGAVIAAAPVPAPGP
                     APGPTAAPAPAAPAQSPPSTAFAVEMVGRPQPSPEEPGFKPHASPSVRRFARELGVDL
                     ALVRGSGPKGRVTPDDVRGFVKGVITGTAARPAPVAPAAGLGLGVLPWPQVDFAKFGP
                     IERVEMSRIKKLSGPNLHRNWVMIPHVTNHEDADITDLEALRVQLNREYEKSGVKITM
                     LAFLIKACVAALKKFPDFNSSIDAEGGAIIRKNYWHIGFAADTPNGLVVPVIKDADRK
                     GIVEIAQEMAALSAKAREGKLSPADMQGGTFSISSLGGIGGTYFTPIINAPEVAILGV
                     CRSSMRPVWDGKAFVPRLILPLSLSWDHRVVDGAGAARFNAYLASVLGDFRRVLL"
BASE COUNT          316 a          690 c          593 g          325 t
ORIGIN      
        1 gccgcgctcg acggaatctc catcgacgcc ttgtcgctct ccaccgtgat cagcgactgc
       61 tccttcgcca ccacgtcgcc ggccttcacc agcacttcga tgacttcgac gtccttgaag
      121 tcgccgatgt ccggcacctt cacttccatc aactggctca tgcggttctc tcttggtctt
      181 ctgcgacgga catgggaaaa ctgaacacgc gcaccggcag cgccgagctc ttgtcgaact
      241 cgcagcccgc cttcacgccg acctcggcga tctggcgcgc gctgcggccg cggtcgtaaa
      301 ccgcgtacat cgccccgagc gcgaaattgc gccccgagcc gatgccccag aacttgtcga
      361 agctgaacac ttcgcgatac gaatacacac cgtagatgcc cgaggcattc gcgatcaccg
      421 ccgtgatctg cgacgactcg tacggatcgg actcgtcttc cttcgggttc aggaagtact
      481 tctccttcag cagcgcgtgc agcctctgaa aggtggcgaa gatctccaca cgcccaccga
      541 gcctgcagtc gccgcccatg ctgcgcagcg cgtcggccag caccgcgaag tgcgcggtgc
      601 tgccggccag cccgatgtac gaatcgccga cggtgatgat cttctcgttg acctcgtagc
      661 cgtgcggcag ccgcgtctcg ccgaacgtca ccagcgcgtc ggacgcgatc gcgatctcgc
      721 ggttcttctt gacgacaacg atcgtggtca cgatctacag aagcacccgc ctgaaatccc
      781 ccagcaccga cgccaggtac gcgttgaagc gcgcggcgcc ggcgccgtcg acgacgcgat
      841 ggtcccacga cagcgacaag ggcaggatca gccgtggcac gaacgccttg ccgtcccaca
      901 ccggccgcat gctcgaacgg catacgccga ggatggccac ttccggtgcg ttgatgatcg
      961 gcgtgaagta ggtgccgccg atgccgccga gcgacgagat cgagaacgtg ccgccctgca
     1021 tgtcggccgg agacagcttg ccctcgcgcg ctttggccga aagcgccgcc atctcctggg
     1081 cgatctcgac gatgcccttc ctgtccgcgt ccttgatcac cggcacaacg aggccattcg
     1141 gcgtgtcggc cgcgaacccg atgtgccaat agttcttgcg aatgatcgcg ccgccctcgg
     1201 cgtcgatcga gctgttgaag tcgggaaact tcttcagtgc cgccacgcac gccttgatca
     1261 ggaacgcgag catcgtgatc ttcacgccgc tcttctcgta ctcccggttc aactgcacgc
     1321 gcaacgcttc caggtcggtg atgtccgcat cctcgtggtt ggtgacgtgc gggatcatca
     1381 cccagttgcg gtgcaggttc ggtcccgaaa gcttcttgat gcgcgacatc tcgacgcgct
     1441 cgatcggacc gaacttcgcg aagtccacct gcggccacgg cagcacgccg aggccgaggc
     1501 cggccgcggg tgcgaccggt gccggccgtg ctgccgtacc ggtgatcacg cctttgacaa
     1561 agccgcgcac gtcgtccggt gtgacccgac ccttggggcc gctgccgcgc accagcgcca
     1621 gatccacgcc gagctcgcgt gcaaagcggc gcaccgacgg cgaggcgtgc ggcttgaagc
     1681 cgggctcctc gggtgacggc tgcgggcggc cgaccatctc gaccgcgaac gcggtcgacg
     1741 gcggggactg cgccggcgcg gccggtgcgg gcgctgccgt gggcccaggg gcaggcccgg
     1801 gcgccggcac gggcgcggca gcgatcaccg cacccgtcgc ggccagcgtc aggatcacgc
     1861 tgccctcgga gaccttgtcg ccgagcttca cccgcatctc atgcaccgtg cccgccgcgc
     1921 tcga
//
LOCUS       DBNHBY010000295         3525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_693878, whole genome shotgun sequence.
ACCESSION   DBNHBY010000295 DBNHBY010000000
VERSION     DBNHBY010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_693878"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..>3525
                     /locus_tag="AC16HB_07245"
     CDS             648..>3525
                     /locus_tag="AC16HB_07245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR5606624.1"
                     /translation="MTALAQLRSTLGAGLRLPLKFRLGLILGVASILLAAGQASLDHY
                     FVSRALVRESSDDIGDTAIRAADALGRSALVLRDSLQAATTFAIGSRPLLAPAAQDAA
                     FIKDTLESLRRQTPQVAGLAVTTVHGKLIARTEDDVAKSNRADDERASALTLSIPIER
                     EGCIDGLLIVRPTSQFFSDIERLALPQSGRAASRQLLIASPTGRVTYGPREMLGVPLP
                     SELFDARSQKTADRLAAWPDRNEYLSASVATVSYRGTVFPDWRVIVRQPASSVLQPAT
                     LSGTGHAVSAAVLTTMFVLLGWLLAMRVAAPLQRISAAVTAWRHGRTDAWIPMIERSD
                     ELGDLSAALRVLLAGMSSQSEALRFSEGRLREIFEASPVSLWVVNFSALRREIDALRK
                     SGQSDIGGLIERDPEFLRSALGLVRVLEVNRHSLQLMQTGLREDLTRSLDAVLDEPSW
                     PLFRQQMMAIASGAAHFEGSGVLTTRRGEPRQVQCSFAVPGTPPDYNRVLVAFADLTE
                     RANAERELRASEERFRLLVDGVHDYSIIMLDADGCVVSWNSGAERIKGYSAAEAIGQP
                     IHIFYPTEEMAAGRPLQLLERAREDGRATDEGWRVRKDGSRFLAHVIITALRDEDGGL
                     YGYSKVTRDVTQLRMAAQRIEHLATHDALTNLPNRLLLHDRLEQSLAAARRSHTLLGL
                     MFIDLDRFKYVNDTLGHEVGDALLKEAAARMSATVRQCDTVARQGGDEFILLLTSLYS
                     RFDVEKIAEKILQRMAKPFVIHGHQVMISASIGIAMFPSDGDDAATLLRNADISMYEA
                     KRAGGNTARFFAAQMSSETMRRTQVEQALRKAIEAGDIDVHFQPQVRLESSELVGFEA
                     LARWCHATLGDIPPTEFIPIAEEIGLIHELGEQILRKACAHAAGWPQRGVPAVRVAVN
                     VSPKQLSQPDFPRLIAAILAESHLPAERLELEITESVAIEFATGS"
BASE COUNT          596 a         1188 c         1172 g          569 t
ORIGIN      
        1 tgacgcggtc gccgatgctt gggctgtagc ggctcgatcg cccgaagcac acctccgtca
       61 ttgcacgcgg gtcgccatac gagcggccgg aattggcagg tccggcccgg cgggcgaacc
      121 gggccattgc gtcgctcgcg atggccggcc gctaatcgcc gtcgaacgca ccgcccatcg
      181 gtcgcggccc ggctcgcaga cggtttgcgg tgcgcaaatt gcgctgcgat agtgggtcct
      241 ggaccgggaa ccagtcaacg cgcccgcctt tcctcttgac tcattcgtgg gcgggggtgt
      301 ccggtccctc tcccggatgt cggctcggac ggcaaggaac tgctgtcgct cggggctcac
      361 agagtgcaga cattcccggt tgcgcttctt caatcgtcag cgggctggcc gcgcgaatac
      421 ctgtcaggaa gccctgcacc cgggaccctt gcccgtcggt cggtgtgtca aggcaaccgg
      481 tcggcctgcg cagccaaata acaaaaagac gggatacata aacacgtgat ggcaacagaa
      541 ctcaaagcac tggacggcgg agtgccggtc ggtccccggc gggccgaccg cgaattccgc
      601 tgccgctgcg gcatccgtgc cggccggtgc ctcccgagag gactgtcttg acggcgctcg
      661 cccaactgag gtccacgctc ggcgccggcc ttcggctgcc gctcaagttc cgactcggtc
      721 tgatcctcgg agtggcatcg atcctccttg cggccggaca ggccagcctc gatcactact
      781 tcgtcagccg cgccctggtg cgcgaatcga gcgacgacat cggcgacacg gcgatccgcg
      841 cggcggacgc gctcggacgc tcggcgctcg tgctgcgcga cagcctgcag gcggcaacga
      901 ccttcgcgat cggctcgcga ccgctgctgg cgccggcggc gcaggatgcc gcgttcatca
      961 aggacacgct cgaatcattg cggcgccaga caccgcaggt tgcggggctg gcggtcacaa
     1021 cggtccacgg caagctgatc gcccggacgg aagacgacgt tgcgaagtcg aaccgggccg
     1081 atgacgaacg cgcgtccgca ctcacactct cgatcccgat cgaacgcgaa gggtgtatcg
     1141 acggactcct gatcgtgcgg ccgacgtcgc agttcttcag cgatatcgag cgcctggcgc
     1201 tgccgcagtc cggccgcgcc gcgagtcggc agctgctgat cgcgtccccc accgggcggg
     1261 tgacctatgg accacgcgag atgctcggcg tcccccttcc atccgaactg ttcgacgccc
     1321 gatcacagaa gacggcagac cggcttgccg catggccgga tcgcaacgag tatctgagcg
     1381 cttccgtcgc gacggtcagc tacaggggaa cggtgtttcc cgactggcgg gtcatcgtcc
     1441 ggcagccggc gtcaagcgtt ctgcagcccg ccactctgag cggaaccgga cacgcagtgt
     1501 cggcggccgt cctgacgacc atgttcgtgc tgcttggctg gctgcttgcg atgcgcgtgg
     1561 cagcaccgct gcagcggatc tcggcggcgg tcacagcctg gcgacacggc cgcacggacg
     1621 catggattcc catgatcgag cgaagcgacg aactgggcga tctgtcggcg gcgctgcgcg
     1681 tactcctcgc cggcatgtcc agccaatcgg aagcgctgcg gttcagcgaa ggacgcctgc
     1741 gcgagatctt cgaggcgagc ccggtgtcgc tatgggtggt caatttctcg gccctgcgcc
     1801 gcgagatcga cgcgctgcgc aagtcgggcc agtcggacat cggcggcctg atcgaacgcg
     1861 acccggagtt cctgcggagc gcactcgggc tggtccgggt gctcgaagtc aaccgtcact
     1921 cgctgcaact gatgcagacc ggcctgcgcg aagacttgac ccgttcactc gatgccgtgc
     1981 tcgacgagcc gtcctggccg ctgttccgtc agcagatgat ggcgatcgcc tccggcgcgg
     2041 cgcatttcga gggcagcggc gtgctcacga cccggcgcgg cgagccgcgc caggttcaat
     2101 gcagctttgc cgtcccgggg actccccccg attacaaccg cgtgctggtc gcgttcgccg
     2161 acctgacgga gcgcgccaat gccgagcgcg aattgcgcgc gagcgaagaa aggttccgcc
     2221 tgctcgtcga tggcgtgcac gactactcga tcatcatgct ggacgccgac ggctgcgtgg
     2281 tgagctggaa ctcgggcgcc gagcgcatca agggctatag cgccgccgag gccatcggcc
     2341 agccgatcca catcttctat cccaccgagg agatggccgc cggaaggccg ttgcagctgc
     2401 tcgagcgagc gcgcgaagac gggcgcgcca ccgacgaagg ctggcgcgtg cgcaaggatg
     2461 gttcgcgctt cctcgcccac gtcatcatca ccgcgctgcg cgatgaagac ggcggactgt
     2521 acggctactc gaaggtcacg cgcgatgtga cgcaactgcg catggccgcg cagcggatcg
     2581 aacatctggc cacgcacgac gccctgacca atctgccgaa ccggctgctg ctgcacgacc
     2641 ggctcgaaca gtcgctggcg gcagcgcgtc gttcgcacac gctgcttggc ctgatgttca
     2701 tcgatctgga ccgcttcaag tacgtgaacg acacgcttgg ccacgaagtc ggcgacgccc
     2761 tgctgaagga ggccgccgcg cgcatgtccg cgacggtccg ccagtgcgac accgtggcgc
     2821 gccagggcgg cgatgaattc atcctgctgc tgacgtcgct gtactcgcgg ttcgacgtcg
     2881 agaagatcgc cgagaagatc ctgcagagga tggcgaagcc gttcgtgatc cacgggcacc
     2941 aggtcatgat ctcggccagc atcggcatcg cgatgtttcc cagcgacggc gacgatgcgg
     3001 ccacgctgct gcgcaatgcg gacatttcga tgtacgaggc caagcgcgcg ggtggaaaca
     3061 cggcgcgctt cttcgccgcg cagatgagtt cggagacgat gcgtcgcacg caggtcgagc
     3121 aggcgctgcg caaggccatc gaggccggcg acatcgacgt tcacttccag ccgcaggtgc
     3181 ggctggagtc gagcgaactg gtcggcttcg aggcgctcgc acgctggtgc catgcgacgc
     3241 tgggcgatat cccgccaacc gagttcatcc ccatcgcgga agaaatcggc ctgatacacg
     3301 aactcggcga gcagatcctg cgcaaggcat gcgcgcacgc cgcaggctgg ccgcagcggg
     3361 gcgtgccggc ggtgcgcgtc gcggtcaacg tttcgccaaa acagctgtcc cagcccgact
     3421 ttccgagact gatcgccgcg atcctcgccg agtcgcatct gccggcggag cgtctcgaac
     3481 tcgaaatcac cgaaagcgtc gcgatcgaat tcgcgaccgg ttcca
//
LOCUS       DBNHBY010000296        11228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_152706, whole genome shotgun sequence.
ACCESSION   DBNHBY010000296 DBNHBY010000000
VERSION     DBNHBY010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_152706"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="dapE"
                     /locus_tag="AC16HB_07250"
     CDS             complement(<1..962)
                     /gene="dapE"
                     /locus_tag="AC16HB_07250"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR5606625.1"
                     /translation="MNPTVELTKALIARPSVTPDDAGCQGLIAERLMPLQFGAETIAR
                     NGTTNLWLRRGASRPVVVFAGHTDVVPPGPREQWLSDPFVPAERDGVLYGRGASDMKT
                     SIAAFTVAAAEFVAAHPDHRGSIALLLTSDEEGPATDGTVAVVEKLSARGEAIDFCIV
                     GEPTSVDEVGDTIKNGRRGSLSGRLTVRGVQGHVAYPHLARNPVHELAPALADLAAEV
                     WDRGNEFFPATTFQVSNIQAGTGALNVIPGACVVEFNLRFAPVLTADMLMARIGTILR
                     RHRLDCDINWTVGAQPFMTERGVLSDALSRAIRETTGRATALSTT"
     gene            complement(1018..2157)
                     /locus_tag="AC16HB_07255"
     CDS             complement(1018..2157)
                     /locus_tag="AC16HB_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR5606626.1"
                     /translation="MALDKLFQLMAERKASDLYLSVGSPITIKINGVCVPINQERLQP
                     QQVVQLLASRLSDAQFRELEEDCELNLGLPVQGIGSFRLSAFMQRGTVSAVVRFIPHD
                     VPRLDELSLPEMLKDLVMEKRGLILVVGAAGSGKSTTIASMLDHRNELATGHMLTFED
                     PIEFLFRNKKSIINQREIGSDAKTLQTALKNAMRQAPDVIFIGEIRDRDTMGAALAYS
                     MSGHLVVATMHATNCAHALNRVISFYSPETREALFQDMAASLKAIVSQRLVRSRKGGR
                     VPTVEVLLNTGHATELVERGDVQGIKEAMEQTLAPGSQTFEQSLYTMLQKDLITREDA
                     LTAADSKNNLLWLINNAGKAQSQGSGQQKKLAAEPSSFSEFTLNI"
     gene            complement(2293..3132)
                     /gene="dapD"
                     /locus_tag="AC16HB_07260"
     CDS             complement(2293..3132)
                     /gene="dapD"
                     /locus_tag="AC16HB_07260"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR5606627.1"
                     /translation="MSMQSIIEEAWENRAGLTPGNAPAALKDAVNAAIDDLDAGRARV
                     AAKHGGEWVVHQWLKKAVLLSFRLRDNRRMGFDHAPGVGPAFQFYDKVDTKFAGWSDA
                     DFQRAGVRVVPPAVARRGAYLANNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGR
                     NVHLSGGVGIGGVLEPVQANPTIIEDNCFIGARSEVVEGVIVEENSVISMGVFIGKST
                     RIYDRESGEILYGRVPAGSVVVSGSLPSKNCQLYCAVIVKRVDAQTRAKTGINELLRE
                     AAA"
     gene            complement(3141..4325)
                     /gene="dapC"
                     /locus_tag="AC16HB_07265"
     CDS             complement(3141..4325)
                     /gene="dapC"
                     /locus_tag="AC16HB_07265"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR5606628.1"
                     /translation="MNPDLQRLQPYPFERLRRLYEGVTPPADLKLIDLSIGEPKHPTP
                     RLIFDALTEALPGLASYPSTIGTEALRESIAHWLMRRYLVPRVDPATQVLPVCGSREA
                     LFAIAQALIDRSRDPLVISPNPFYQIYEGAALLAGAQPLFLPVTPANGFRMEFERVSA
                     DVWRRVQLVYVCTPGNPHGRVMTQDEWAALFELADRHGFVIAADECYSEVYFDEARPP
                     LGALQAAHALGRDRYDRLLVFGSLSKRSNAPGLRSGFVAGDAALLKQFLLYRTYHGNA
                     MSPPVQAASIAAWNDERHVIENRRRYAEKFDMATPIVQRALRVERPEAAFYLWAETPS
                     DDEAFARRLYAECHVKALPGSYLSRERDGLHPGKGFVRLALVASEAEVLEAAQRIATM
                     KR"
     gene            complement(4322..4741)
                     /gene="queF"
                     /locus_tag="AC16HB_07270"
     CDS             complement(4322..4741)
                     /gene="queF"
                     /locus_tag="AC16HB_07270"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR5606629.1"
                     /translation="MATRPSKTLETFANPSPARDYLVHIEIPEFTCLCPLTGQPDFAT
                     LVLDYVPHRKNVELKSLKLYLWAYRNEGAFHEAVTNRIVDDLVRALAPRFARLTARWY
                     VRGGIFTTVVAEHRKRGWKAAPYVDVGRFEASSSMRG"
     gene            4848..8372
                     /gene="smc"
                     /locus_tag="AC16HB_07275"
     CDS             4848..8372
                     /gene="smc"
                     /locus_tag="AC16HB_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR5606630.1"
                     /translation="MRLRQIKLAGFKSFVDPTHIDVPGSLVGVVGPNGCGKSNVIDAV
                     RWVLGESKAAELRGESMQDVIFNGSANRKPGGRASVELSFENNDGRIGGEWGKFSEIT
                     VKRVLTRDGTSSYYINNQSVRRRDVQDMFLGTGLGPRAYAIIGQGMITRIIEARPDEL
                     RVFLEEAAGVSKYKERRRETENRLADTRDNLTRVEDILRELETQIAKLEQQAEVAQRY
                     HSLKTEHDGNQQLLWYLRRRDAQAEADKIARDIAARANAVEAHVAELRATERHLEEAR
                     SAHYAASDAVHAAQGALLNVNAEIGKLESELRLIADSRMRMASQVETLAAAHGRRLTE
                     HQDLTARAAELARQISDGEQKHASSLNLVDGHAAQLPQLEDAYGVARDAMGAARTVVA
                     MAEQAIDSAGERQRAIDRQLGALAQRRDRLNEERTQLGAPDDARLATLKADAQRMEAE
                     LAAGTQRQQSAEVAVARLQGERQAGIEARTRETDTLARLEARLHALRELQANVEADEK
                     LDPWLRKQGLDSLPRLFRRIHVEAGWEIAFEAVLRERLEGLEVGRLDTLAGLANDAPP
                     ARVSFFSMAGAVAREGVHMPGFARLTEHVRGHDAALAAVLGDWLVNVFVAEDVGQALA
                     ARDQLPSGGQFVVKAGHLVSRYGVRFYAADDRKAGLLARRQEIENLERELRAQKLLVE
                     QSVGALARAEAQLRQAQEQSLTERQAADRLRAQLHASQLEVVRLDELIGRVRHRSGQI
                     EAELAEVTAHESELRAQRGDADGRFEQLDRELAAKQAAFEAARGAYEDADRALREARD
                     EQQRLSSERQRHEFELGAARERAADVAHAIQAALADAERLASDLEATRAQLTQLDDSA
                     VRTGLDRWLAQRTSAEDALRETRSRLDDVGQQLKALDEKRLTLEGELQPKRDKITELQ
                     LKEQAARLSAEQFAQQLADVHADEAALGARLEAAPVKANALQPEIARLAAEIEALGAV
                     NLAALEELNSSRERKQFLDAQSTDLKTAIGTLEDAIRRIDRETRELLQSTFDAVNEQF
                     GILFPKLFGGGEAKLIMTGAEILDAGVQVMAQPPGKRNSTIHLLSGGEKALTATALVF
                     ALFKLNPAPFCLLDEVDAPLDDANTERFCDLVRSMIGDTQFLFITHNKIAMEMAEQLV
                     GVTMQEQGVSRIVAVDIDSAVRLTAEAA"
     gene            8386..9459
                     /locus_tag="AC16HB_07280"
     CDS             8386..9459
                     /locus_tag="AC16HB_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HIR5606631.1"
                     /translation="MNDLHVALGALALALLGGVYGYSKWQERRALRRFQQSLHGSVGD
                     PLLSPGGPPTQPLPPAAPGSRIEPTFGPLPDTGLNVDAAEAAVPTETRPIASNWIEDP
                     LLDCVLELRCAHAVDGVAVIDAAAPLARLRLSLPVHLVTWEGRSQKWVAPDRFGFYSE
                     LLAAVQLANRTQMLSEIEASQFIGAVQQLAVALDADFDPPDTRRVMELAADLDRLCAR
                     FDVRIGLTLEPTDGAWNSERVAAAAAACDLAAITPLRWQQLDAAGEALFSVAAAGTPV
                     DRLLLELDVPRVAPAAQPLRAMFATASQLAAALEARIVDDNGRPVQPASIDAIERQLA
                     SLYQDMLAAGIEPGSARARRLYG"
     gene            9467..>11228
                     /gene="ligA"
                     /locus_tag="AC16HB_07285"
     CDS             9467..>11228
                     /gene="ligA"
                     /locus_tag="AC16HB_07285"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR5606632.1"
                     /translation="MRVTSEAPKRARDRIEELRREIERHDHAYYVLNAPTVPDAEYDR
                     LFAELHTLEGQYPQLVTPDSPTQRVSGAPSSAFAEVVHAVLMLSIRTETDTSDAGAFA
                     FDARIRRELQIGPDDPPVEYCVELKFDGLAISLRYEHGVLVRGATRGNGMVGEDVTAN
                     VRTIRSIPLRLRGAPPLVEVRGEALMLRRDFERLNERQESAGDRRFANPRNAAAGSLR
                     QLDPTITAQRPLHFFAYGVGETRGWAMPATQAALLDALSMLGVPVENHRMVVRGADGL
                     VQFHRRIGAQRTELPFEIDGVVYKVDRIELQQRLGFVTREPRWAVAHKYPAQEMVTTL
                     LGIEVQVGRTGALTPVARLAPVFVGGTTVSNATLHNEDLIREKDIRIGDTVIVRRAGD
                     VIPEIVGTVPERRPMDARPFHMPRRCPACGSAVFREEGEKVARCTGGLVCPAQRKQAL
                     LHFAGRRALDIEGVGDKLVDQLVDAGLVRTPADLFRLGLGALASLERMAEKSAANVLA
                     AIDKARHTTLDRLVFALGIRHVGESTARDLAAHFGSLDRLIAADEAQLLEVPDVGPVV
                     AASIVRFFAEPHNREAIEQLR"
BASE COUNT         1828 a         3746 c         3933 g         1721 t
ORIGIN      
        1 gtggtcgaca gggcggtcgc gcggccggtc gtctcgcgga tcgcgcgcga cagcgcgtca
       61 ctgagcacgc cgcgctcggt catgaacggc tgcgcgccga cggtccagtt gatgtcgcag
      121 tcgagccggt gccggcgcag gatagtcccg atccgcgcca tcagcatatc cgccgtcaac
      181 acaggcgcga agcgcaggtt gaactcgacc acgcaggccc cggggatcac gttcaacgcg
      241 ccggtgccgg cctgaatgtt cgagacctgg aacgtcgtgg ccggaaagaa ctcgttgccg
      301 cgatcccaga cctcggctgc gagatcggcg agtgcgggcg ccagttcgtg gaccggattg
      361 cgcgccaggt gtgggtaggc gacatggccc tgcacgccgc gcaccgtgag ccggcccgac
      421 agcgagccgc ggcggccgtt cttgatcgtg tcgccgacct cgtcgaccga ggtcggctcg
      481 ccgacgatgc agaagtcgat ggcctcgccg cgcgccgaaa gtttctcgac gacggcgacc
      541 gtgccgtcgg tggccgggcc ttcctcgtcg gaggtgagca gcagcgcgat ggagccgcga
      601 tggtccggat gcgctgccac gaattccgcg gcggcgaccg tgaacgcggc gatcgacgtc
      661 ttcatgtccg aggcgccgcg gccgtagagc acaccgtccc gctcggccgg tacaaacgga
      721 tccgacagcc actgctcgcg cggcccgggt ggcaccacgt cggtgtggcc ggcgaacacg
      781 acgaccggcc gcgaggcgcc gcgccgcagc cacagattgg tcgtgccgtt gcgggcgatg
      841 gtttcggcgc caaactgaag cggcatgagc cgctcggcaa tcagcccctg gcagcccgcg
      901 tcatcgggcg tgaccgaggg ccgcgcaatc agcgctttgg tgagttcgac agtgggattc
      961 atccggcacg ccgcgcgcgg aaagccgcgc ggtccggccg gattctatcg agatgttcta
     1021 gatgttgagc gtgaattcgg agaacgacga cggctcggcc gccagcttct tctgttgacc
     1081 cgagccctgc gactgcgcct tgccggcgtt gttgatcagc cagaggaggt tgttcttcga
     1141 gtccgcggcc gtcagcgcgt cctcgcgcgt gatcaggtcc ttctgcagca tcgtgtacag
     1201 gctctgctcg aaggtctggc tgcccggtgc gagcgtctgc tccatcgctt ccttgatccc
     1261 ctgcacgtcg ccgcgttcga cgagttcggt cgcgtgtcca gtgttcagca acacctcgac
     1321 cgtgggaacg cgtccgccct tgcgcgagcg caccagccgc tgcgagacga tcgccttcaa
     1381 tgacgcggcc atgtcctgga acagcgcctc gcgcgtctcc ggcgagtaga agctgatgac
     1441 gcggttcaag gcgtgcgcgc agttggtcgc gtgcatcgtc gcgaccacca ggtggcccga
     1501 catcgagtag gccagcgcgg cgcccatcgt gtcgcggtcg cggatctcgc cgatgaatat
     1561 cacgtcgggc gcctgccgca ttgcgttctt cagcgcggtc tgcagcgtct tggcgtccga
     1621 gccgatctcg cgctgattga tgatcgactt cttgttgcgg aacaggaact cgatcgggtc
     1681 ctcgaaggtc agcatgtgcc cggtcgcgag ttcattgcgg tgatcgagca tcgacgcgat
     1741 cgtggtcgac ttgcccgagc cggccgcgcc gacgaccaga atcagtccgc gcttttccat
     1801 cacgaggtcc ttcagcatct ccggcagcga cagctcgtcg agccgcggca cgtcgtgcgg
     1861 gatgaagcgg accacggccg acaccgtgcc gcgctgcatg aacgcgctca ggcggaagct
     1921 gccgatgccc tggacgggaa ggccgaggtt cagttcacag tcctcctcca gctcgcggaa
     1981 ctgcgcgtcc gacagccggc ttgccagcag ctggaccacc tgctgcggct gcaggcgctc
     2041 ctggttgatc ggcacgcaga cgccgttgat cttgatcgtg atgggcgaac ccaccgacag
     2101 gtacaggtcc gatgccttgc gctcggccat cagctggaac agcttgtcga gggccatgtg
     2161 tcctccagtc ttcggaaggt caagaatacg cagcgtggtg cggggctgga aatcgacaga
     2221 ttgggctaag tgtgcgcctg tccatgggcg gattccagcg cgtgcctgcg caccacgcgt
     2281 cgcgggcccg gatcaggcag ccgcctcgcg cagcagctcg ttgatgccgg tcttggcgcg
     2341 ggtttgcgcg tcgacgcgct tgacgatcac cgcgcagtag agctggcagt tcttcgacgg
     2401 cagcgaaccc gagacgacga ccgacccggc cggtacccgg ccatacagga tctcgccgct
     2461 ttcgcggtcg tagatccgcg tgctcttgcc gatgaacacc cccatgctga tgacggagtt
     2521 ctcctcgacg atcacgcctt cgactacttc ggagcgggcg ccgatgaagc agttgtcctc
     2581 gatgatcgtt gggttcgcct gcaccggctc cagcacgccg ccgatgccga ccccgcccga
     2641 caggtgcacg ttcctgccga tctgcgcaca cgagccgacg gtggcccagg tgtcgaccat
     2701 cgtgccctcg tccacgtagg cgccgatatt cacgtaggag ggcatcagaa ccacgttgtt
     2761 cgccagatag gcgccgcgcc gggccacggc cggcggcacc acgcgcacgc cggcgcgctg
     2821 gaagtccgca tcgctccagc cagcgaactt cgtgtcgacc ttgtcgtaga actgaaacgc
     2881 cggccccacg cccggcgcgt ggtcgaagcc catgcggcgg ttatcgcgca ggcggaatga
     2941 cagcagcacc gccttcttca gccactggtg cacgacccat tcgccaccat gctttgccgc
     3001 gacccgcgcg cggccggcgt ccagatcgtc gatcgccgcg ttgaccgcat ccttcagcgc
     3061 ggcgggtgca ttgcccggag tcaggccggc gcggttctcc cacgcctctt cgatgatcga
     3121 ttgcatcgac atgagggtcc tcagcgtttc atggtggcta tgcgttgcgc ggcttcgagc
     3181 acttcggcct cgctggcgac cagcgcgagc cgcacgaacc ccttgcccgg gtgcagcccg
     3241 tcgcgctccc gggacaggta actgcctggc agcgccttga cgtggcattc ggcgtaaagt
     3301 cgccgcgcga aggcttcatc atcgctcggc gtttcggccc acaggtagaa cgccgcttcg
     3361 ggacgctcga cgcgcagcgc gcgctgtacg atcggcgtgg ccatgtcgaa cttctcggcg
     3421 tagcggcggc ggttctcgat cacgtgccgc tcgtcattcc aggccgcgat gctggctgcc
     3481 tgcaccggcg gactcatcgc gttgccatgg taggtgcggt agagcagaaa ctgcttcagc
     3541 agcgcggcgt cgccggcaac gaaaccggag cgcagccccg gcgcgttcga gcgcttcgac
     3601 aggctgccga atacgagcag gcggtcgtag cggtcgcgcc cgagcgcgtg cgccgcctgc
     3661 agagcgccga gcggtggtct ggcttcgtcg aagtagacct ccgagtagca ctcgtcggcg
     3721 gcgatcacga agccatggcg gtcggccagt tcgaacagcg ccgcccattc gtcctgcgtc
     3781 atcacgcgcc cgtgcggatt gccgggcgta cagacgtaga cgagctgcac gcgccgccat
     3841 acgtcagcgc tcacgcgctc gaactccatg cggaatccgt tcgcgggcgt gaccggcagg
     3901 aacagcggct gggcgcccgc cagcaacgcg gcgccctcgt agatctgata gaaggggttg
     3961 ggcgagatca ccagcgggtc gcggctgcgg tcaatgagcg cctgcgcgat cgcgaacagc
     4021 gcctcgcgcg agccgcagac cggcagcacc tgcgtcgccg ggtcgacgcg cggcacgagg
     4081 tagcgccgca tcagccagtg cgcgatcgat tcgcgcagcg cctcggtgcc gatcgtgctg
     4141 ggatagctcg ccagcccagg cagggcctcc gtcagtgcgt cgaagatcag ccgcggcgtc
     4201 ggatgcttgg gctcgccgat cgacaggtcg atcagcttca ggtccgcggg cggggtgacg
     4261 ccttcataca ggcgccgcag gcgttcgaac ggatacggct gcagccgctg caggtcggga
     4321 ttcatccgcg catgctcgac gaggcttcga accggccgac atcgacgtac ggcgccgctt
     4381 tccagccccg cttccggtgc tccgcgacga ccgtggtgaa gatgccaccg cgcacgtacc
     4441 accttgcggt caggcgcgcg aagcgcggcg ccagcgcgcg caccagatcg tccacgatgc
     4501 ggttcgtcac cgcctcgtgg aatgcgccct cattgcggta cgcccacagg tagagcttca
     4561 ggctcttcaa ttccacgttc ttccggtgcg gcacgtagtc gagcaccagc gtcgcgaaat
     4621 ccggctgacc cgtcagcggg caaaggcagg tgaactcggg gatctcgatg tggaccaggt
     4681 agtcgcgcgc cggactgggg ttggcgaagg tctccagcgt tttcgaggga cgtgttgcca
     4741 tctcggcggc gcgatcgagg gtgattcagt tgctatgatt ctacggaatc gactcaactt
     4801 cgcgcggcga cgacgcaaac atcgcagcgc ggtcccggaa cctcatcgtg cgtctacggc
     4861 agatcaagct cgccggcttc aagtcgtttg tcgatcccac ccacatcgat gtgcccggca
     4921 gcctggtcgg cgtggtcggc cccaacggtt gcggcaagtc gaacgtgatc gacgcggtgc
     4981 gctgggtgct cggcgagagc aaggccgccg agctgcgcgg cgaatcgatg caggacgtga
     5041 tcttcaacgg gtcggccaat cgcaagcccg gcggccgcgc cagcgtcgag ctttcgttcg
     5101 agaacaacga cggccgcatc ggcggcgaat ggggcaagtt cagcgagatc accgtcaagc
     5161 gggtgctgac gcgcgatggc acctccagct actacatcaa caaccagagc gtgcgccggc
     5221 gcgacgtgca ggacatgttc ctcggcacgg ggctgggccc gcgcgcctat gcgatcatcg
     5281 gccagggcat gatcacgcgc atcatcgagg cgcgccccga cgagctgcgc gtgttcctcg
     5341 aggaagccgc cggcgtctcg aagtacaagg agcggcggcg cgaaaccgag aaccgtctgg
     5401 ccgacacgcg cgacaacctg acgcgagtcg aggacatcct gcgcgagctg gaaacgcaga
     5461 tcgccaagct cgagcagcag gccgaagtcg cgcagcgcta tcactcgctc aagaccgagc
     5521 acgacggcaa ccagcagctg ctgtggtatc tgcggcggcg tgacgcacag gcggaggccg
     5581 acaagatcgc gcgcgatatc gccgctcgcg ccaacgcagt cgaggcgcac gtggcagaac
     5641 tgcgcgcgac cgagcgccac ctcgaagaag cacgcagcgc gcactatgca gcctccgacg
     5701 ccgtgcacgc ggcgcagggc gcgttgctga acgtgaacgc ggagatcggc aagctcgaat
     5761 ccgagctgcg cctgatcgcc gacagccgca tgcggatggc gtcgcaggtc gagacgctgg
     5821 cagcggcgca cggccgccgc ctgaccgagc accaggacct gaccgcgcgc gccgcagagc
     5881 tggcgcggca gatcagcgac ggcgagcaga agcacgcgag ttcgctcaat ctggtcgatg
     5941 ggcacgccgc gcagctgccg cagctggaag atgcatacgg cgtggcgcgc gacgcgatgg
     6001 gcgcggcgcg caccgtcgtc gcgatggccg aacaggcgat cgacagcgcc ggcgagcggc
     6061 agcgtgcaat cgatcgacag ctgggtgcac tggcacaacg gcgcgaccgg ctgaacgagg
     6121 agcgcacgca gctcggtgcg cccgacgatg cgcgactggc gacgctgaag gcggatgcgc
     6181 agcggatgga ggccgaactt gctgccggca cgcagcggca gcagtcggcc gaggtcgcgg
     6241 tggcgcggct gcaaggcgag cggcaggccg ggatcgaggc gcgcacgcgc gaaaccgaca
     6301 cgctggcgcg gctcgaggca cggttgcacg cgctgcgcga actgcaggcg aatgtcgagg
     6361 ctgacgagaa gcttgatccc tggctgcgga agcagggact ggacagcctg ccgcgcctgt
     6421 tccgccgcat tcacgtcgag gcgggttggg agatcgcgtt cgaggcggtg ctgcgcgagc
     6481 ggctcgaagg cctcgaggtc ggccgtctcg acacgctggc cggactggcc aacgacgcgc
     6541 cgccggcccg ggtgtcgttc ttttcgatgg cgggcgccgt cgcgcgcgag ggcgtgcaca
     6601 tgccgggctt cgcacggctg accgaacatg tgcgcgggca cgatgcggcg cttgcagccg
     6661 tactcggcga ctggctcgtc aacgtgttcg tcgccgagga cgtcgggcag gcgctggcgg
     6721 cacgggacca gctgccgtcg ggtggacagt tcgtggtcaa ggcgggccat ctggtgtcgc
     6781 gttacggcgt gcgcttctac gcggccgacg atcgcaaggc cggcctgctc gcgcggcggc
     6841 aggagatcga gaatctcgag agagagctgc gcgcgcagaa gctgctggtc gagcagtcgg
     6901 tcggcgcact ggcgcgtgcc gaggcgcagc tacggcaggc gcaagagcag agcctgaccg
     6961 aacgacaggc ggcggataga ctgcgcgccc agttgcacgc gtcgcagctc gaggtggtgc
     7021 ggctggacga actgatcggc cgcgtgcggc accgctcagg tcagatcgag gccgaactgg
     7081 cggaagtgac ggcgcatgaa agcgagctgc gggcgcagcg cggcgatgcc gacggccgct
     7141 tcgaacagct cgaccgcgag ctggcggcca agcaggctgc attcgaggcc gcgcgcggcg
     7201 cctacgagga cgccgatcgc gcgctgcgcg aggcacgcga cgagcagcaa cgcctttcca
     7261 gtgaacgcca gcgccacgag ttcgagcttg gagccgcgcg tgagcgcgcc gccgacgtcg
     7321 cacacgcgat ccaggccgcg ttggccgatg ccgagcgtct ggcgtccgat ctcgaagcca
     7381 cgcgcgcgca gctcacgcaa ctcgacgaca gtgccgtgcg cacgggactc gaccggtggc
     7441 tggcccagcg gacgagtgcg gaagacgcgc tgcgcgagac gcgcagtcgg ctcgacgatg
     7501 tcggccagca actgaaggcg ctcgacgaga agcggctcac gctggaaggc gagctgcagc
     7561 cgaagcgcga caagatcacc gagctgcagc tcaaggagca ggccgcgcgg ttgtcggccg
     7621 agcagtttgc gcagcaactc gccgacgtgc acgccgatga agcggcgctc ggcgcgcggc
     7681 tcgaagccgc gccggtcaag gcgaacgcgt tgcagcccga gatcgctcgg ctcgccgccg
     7741 agatcgaggc gctcggtgcg gtcaacctgg cggcgctgga ggagctgaat tcgtcgcgcg
     7801 agcgcaagca gttcctcgac gcgcagagca cggacctcaa gaccgcgatc gggacgctgg
     7861 aggacgcgat ccgccgcatc gaccgcgaga cgcgcgaact gctgcagagc actttcgacg
     7921 cggtcaatga acagttcggc atcctgttcc cgaaactgtt cggcggcggc gaggcgaagc
     7981 tcatcatgac cggcgcggag attctcgacg ccggggtgca ggtgatggcg cagccgcccg
     8041 gcaagcgcaa cagcacgatt cacctgcttt cgggcgggga gaaggcgctg accgccaccg
     8101 cgctggtgtt tgcactcttc aagctgaacc cggcgccatt ctgtctgctc gacgaggtcg
     8161 acgcgccgct ggacgacgcc aataccgagc gtttctgcga cctcgtgcgt tcgatgatcg
     8221 gcgacacgca gttcctgttc atcacgcaca acaagatcgc gatggaaatg gccgagcaac
     8281 tcgtcggcgt gacgatgcag gagcagggcg tttcgcgcat tgtcgcggtg gacatcgaca
     8341 gcgcggtccg gctcacggcc gaggctgcat gagcgacgcg gcccgatgaa cgacctgcac
     8401 gtcgccctcg gtgcgctcgc gctggcgctg ctgggcggcg tctacggcta cagcaagtgg
     8461 caggagcggc gtgccctgcg ccgttttcag cagtcgctgc acggttcggt cggtgatccg
     8521 ctgctttcac ccggcgggcc accgacgcag ccgctgccgc cagcggcccc cgggtcgcgc
     8581 atcgagccga ctttcggccc gctgccggac accggtctca acgtcgatgc ggcggaagca
     8641 gcggtgccga ccgagacgcg accgattgca tcgaactgga tcgaggatcc gctgctcgac
     8701 tgcgtgctcg aactgcgctg cgcgcacgcg gtcgacggcg tggcggtgat cgatgccgcc
     8761 gcgccgctgg cgcggctgcg cctgtcgctg ccggtgcatc tggtcacatg ggagggtcgg
     8821 tcgcagaaat gggtggcacc cgaccgcttc ggcttctact cggaactgct ggcggccgtg
     8881 cagcttgcga atcgtacgca gatgctgagc gagatcgagg catcccagtt catcggtgca
     8941 gtccagcagc ttgcggtcgc gctggacgcc gacttcgatc cgcccgacac gcgccgcgtg
     9001 atggaactgg ccgcggacct tgaccggctg tgcgcgcgtt tcgacgtgcg catcggcctg
     9061 acgctggagc cgaccgacgg cgcctggaat tccgaacgcg tggcggctgc ggcggcggcc
     9121 tgcgacttgg cggcgatcac gccgttgcgc tggcagcaac tggacgcggc gggagaagcg
     9181 ctgttctcgg tggccgccgc gggtacgccc gtcgaccggc tgctgctcga actcgatgtg
     9241 ccgcgcgtcg caccggccgc gcaaccgctg cgcgcaatgt tcgcgaccgc ttcgcagttg
     9301 gcggcggcgc tcgaggcgcg catcgtcgac gacaacggca ggccagtcca gccggcatcg
     9361 atcgacgcga tcgagcgcca gctggcgtcg ctgtaccagg acatgctggc agcgggcatc
     9421 gagcccggct cggcgcgcgc gcggcgcctg tacggctgag cacccgatgc gggtgacgtc
     9481 cgaggcgccg aagcgcgcgc gcgaccgcat cgaggaactg cgccgcgaga tcgagcgcca
     9541 cgaccacgcc tactacgtac tgaacgcgcc gacggtgccc gacgcggagt acgaccgcct
     9601 cttcgccgag ttgcacacgc tcgaagggca gtacccacaa ctcgtcacgc ccgactcgcc
     9661 gacccagcgt gtcagcggcg cgccgagcag cgccttcgcg gaggtcgtgc acgcggtgct
     9721 gatgctgtcg atccgcaccg agaccgacac cagcgacgca ggcgcgttcg cgttcgatgc
     9781 gcgcatccgg cgcgagctcc aaatcgggcc tgatgatccg ccggtcgagt attgcgtcga
     9841 gctgaagttc gacggcctcg ccatcagcct gcgttatgag cacggcgtgc tggtacgggg
     9901 cgccacgcgc ggcaacggca tggtcggcga ggatgtcact gcgaacgttc gcacgatccg
     9961 atcgataccg ctgcggctgc gcggtgcgcc gccgctcgtc gaagtgcgcg gcgaggcact
    10021 gatgctccgc cgcgatttcg agcggctgaa cgagcggcag gaaagcgcgg gtgacaggcg
    10081 gttcgccaat ccgcgcaacg cggccgcggg gagcctgcgt cagctcgatc ccacgatcac
    10141 ggcgcagcgg ccactgcact tcttcgccta cggcgtcggc gagacgcgtg gctgggcgat
    10201 gcccgcgacg caggcggcgc tactcgatgc gctgtcgatg ctcggcgtcc cggtcgagaa
    10261 tcaccgaatg gtcgtgcgcg gcgcggacgg gttggtccag ttccatcgcc gcatcggtgc
    10321 gcagcgcacc gaactgcctt tcgagatcga cggcgtcgtc tacaaggtcg accggatcga
    10381 actgcagcag cggctcggct ttgtcacgcg cgagccgcgc tgggccgtgg cgcacaagta
    10441 cccggcccag gagatggtga ccacgctgct cggcatcgag gtgcaggttg gacgcaccgg
    10501 cgcactgact ccggtcgcgc ggctggcacc ggtgttcgtc ggcggcacca cggtgtccaa
    10561 cgccacgctg cacaacgagg acctgatccg cgaaaaggac atccgcatcg gcgacacggt
    10621 gatcgtccgg cgcgcgggcg acgtgatacc ggagatcgtc ggcacggtgc ccgagcgccg
    10681 gccgatggac gcgcggccgt ttcacatgcc gcggcgttgt cccgcctgtg gctcggcggt
    10741 tttccgcgag gaaggcgaga aggtcgcgcg ctgcacgggc ggactcgtgt gtcccgcgca
    10801 gcgcaagcag gcgctgctgc atttcgccgg ccggcgcgcg ctcgatatcg aaggcgtcgg
    10861 cgacaagctc gtcgatcagt tggtcgatgc cgggctggtg cgcacgccgg ccgatttgtt
    10921 ccggctcgga ctgggcgcgc tggcgagcct ggagcggatg gcggaaaaat ccgcggccaa
    10981 cgtgctggcc gcgatcgaca aggcgcgtca cacgacgctc gaccggctgg tcttcgcgct
    11041 cggcattcgc catgtcggcg agagcaccgc ccgcgatctc gccgcgcact tcggttcgct
    11101 ggaccgcctg atcgcggccg acgaggcaca gttgctcgag gtgcccgacg tcggaccggt
    11161 ggtggcggcg tcgatcgttc gcttcttcgc cgaaccgcac aatcgcgagg cgatcgaaca
    11221 gctccggg
//
LOCUS       DBNHBY010000297         8551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_829241, whole genome shotgun sequence.
ACCESSION   DBNHBY010000297 DBNHBY010000000
VERSION     DBNHBY010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_829241"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..331
                     /locus_tag="AC16HB_07290"
     CDS             107..331
                     /locus_tag="AC16HB_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR5606633.1"
                     /translation="MNWEVIEGNWKQFKGHVKEKWGKLTDDNLDRIAGKRDQLAGRIQ
                     ETYGITKDQAEQQLKAFEQVHKDYQPKNST"
     gene            549..1688
                     /locus_tag="AC16HB_07295"
     CDS             549..1688
                     /locus_tag="AC16HB_07295"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR5606634.1"
                     /translation="MTNRIVFLVPGFFGFTSVGAVSYFEDVEQVLGRALRRRGVEARI
                     VRCITQPTASIPRRTQALRRQVFDAGGLKARELHFVGHSTGGLDVRMLLTPGVKIAGD
                     GSEERIARLTRTCISVATPHHGTPLANHFASVQGQTLLLVLTALATSGQGRGAILAAA
                     KAIAMVARIDDWLGRKDTSLDRIAERLLRKMRFDRRDPVWKYLGEIERDQGAVLQLTP
                     EGIELFDAAVADRAGIDYGCVVAGVPKPRERFKLKELLDPEYVALRMLFRLLHALTAQ
                     AHPRYPYPQITKPTQRVLDRGLGFEATPSISDGIVPTLSQLHGLVLHVARADHLDVVG
                     HYTLAGKGSGDWLPSGAGFTKEAFDATWEAVAAAIARRRRPARAD"
     gene            complement(1705..1962)
                     /locus_tag="AC16HB_07300"
     CDS             complement(1705..1962)
                     /locus_tag="AC16HB_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606635.1"
                     /translation="MLTAYERGWSKLKNGDLLDLAEKEGFAVLVTTDANLKYQQNLAS
                     RQIAIVVLGTTSWPRMRRAASAVVAAVDGATAGSYTEVEIP"
     gene            complement(2026..2247)
                     /locus_tag="AC16HB_07305"
     CDS             complement(2026..2247)
                     /locus_tag="AC16HB_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5606636.1"
                     /translation="MIDWSTCAAVERDPERVSGVWVFRGTRVPVAALFENLEDGALVS
                     EFVNWFPGVTIEQVRAVLEHAARSALATA"
     gene            complement(2398..7659)
                     /locus_tag="AC16HB_07310"
     CDS             complement(2398..7659)
                     /locus_tag="AC16HB_07310"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR5606637.1"
                     /translation="MPDDRRTLIVNRGQVETVLGDTERLLSVAPQRFSDWVRRWHALE
                     AQAELLFDIATTFAGERGDADESEVLLWAIEFRANVASGVRDIEPLVLAMKRLPAMGS
                     TPGAAHALLLELLDRHLRVDASPGDVGMSVPAIVAGLREGMAPEATILAVARWGAACA
                     DHTMQMARLADRARRLVAEMDFAFLFDVNRQLFPIGYRVDAAAPDDSFYDLLASEARL
                     ASFVAIAKGDVASTHWFHLGRAATTVHRRPVLLSWSGSMFEYLMPSLVLDTPHGTLLD
                     QTCRGVVARQIEYGNENSIPWGISESAYNQRDRALTYQYSDFGVPGLGLKRGLGEDLV
                     VAPYATAMAAMFDAPAAARNLLRLQTEGGVGRYGPFEAIDYTPARLADGQSAAVVRAW
                     MAHHQGISLVALGNSLRDGIMRHRFHGDPLAHAAELLLQERPPREVANHRPRLEHLPA
                     TPVREAARPAMRQFDTAHTPIPATHLLSNGRYTVMMTAAGSGYSQRGKLVVSRWREDA
                     TTDGWGSYLYLRDVDSGKLWSATYQPGAVEPDDYRVVFAEDRARFTRRDGALTTVLEV
                     IVSSEDDAEVRRLSITNNGSRAREVEITSYAEVVLTEPAADIAHPAFSNLFVQTEFVP
                     EVRGLLATRRTRSPEEPALWVAHVVTRDRANADGLEYETDRARFLGRGRTVRTPAAVL
                     DGHPLSNTVGPVLDPVLSLRTRVRLAPGATVHTVFSTMVAASRDAVLDLADKYHDAST
                     FERISTLAFTHAQVQLHHLGIEADDAALYQELGSRILFSDPAVRPASDELKRNTLSTA
                     GLWPHGISGDHPIVLLCIDAVEDRGIVAQLLRAHEYWQMKGLAVDLVLLNDKASSYLQ
                     DLQMLLEDMARSSRATIGQDAQGPLGRVFVLRADLLGRDERALLHTVARAVLIGSQGG
                     LAEQILRLQRFNAAAEPAKRSEVTRRSADAPAPQLPPLEFFNGLGGFADAGREYVIVQ
                     GPRQHTPMPWLNVVANPDFGFQVSETGAGYTWSQNSRENQLTAWSNDPVTDPPGEVLY
                     VRDEDSGELWTPTAAPIRIEHATYVARHGQGYSRFQLDYAGIQCDWLQCVSWDDPVKL
                     SRLTLVNRSSRPRRLSITAYVEWVLGTTRSKSAPYVVTERDATTGAVFARNPLHDEFG
                     ARVAFVDLGGSPTSYTCDRGEFLGRHGSLERPAALTAEVALGNRAGAGLDPCCALQTV
                     LELKPGQRASLVFLLGQAADRETARGLVLRYRAVDVDAVLAEIRRNWDRILGTVQVET
                     PDRSMDLMLNRWLQYQTIVCRLWARAAFYQAGGAYGFRDQLQDTMALTLALPAAARAQ
                     LLRAAARQFVEGDVQHWWHPPTGRGVRTRCADDLVWLPYAAAHYVAVTGDAAVLDEQL
                     PFLEGQRLEPGQHDAYFTPTVSQQIGTLFEHCERALDRSLPVGAHGLPLIAGGDWNDG
                     MNRVGHLGKGESVWLAWFLFATLQKFATLADSRGASELAARWRAHAASLQAAAESQAW
                     DGAWYRRAYFDDGTPLGSAGNSECRIDSIAQSWGVLSGAAEPERARRAMEAVDAYLVR
                     PGDDLVLLFAPPFDKTENAPGYIKGYLPGVRENGGQYTHAAVWCVVACARLGDGERAA
                     ELFAMLNPVNRAASRAGVYAYKVEPYVVAADIYAEPPHVRRGGWTWYTGAAGWLYRAG
                     TESMLGITKRGDVLHVAPCIPPHWSGFKVRYRHGATDYEVSVENPRGVARGVATIELD
                     GERLERPEHGIALRDDGQTHRVDVVLG"
     gene            complement(7635..>8551)
                     /locus_tag="AC16HB_07315"
     CDS             complement(7635..>8551)
                     /locus_tag="AC16HB_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606638.1"
                     /translation="FISLPPRRAAAEPLSAAQVALLRGTARRIWLFFTKFVTAEDHAL
                     PPDNFQQTPEPVVAHRSSPTNFGLYLLSCVAARDFGWIGLAELADRIEATSDTLLRLP
                     RYRGHFFNWYETRELRPLEPKYVSTVDSGNLAGHLLAVAQGCDECIRQPLFARNALDG
                     LGDALQLARAALRDMPDDRRTLIVNRGQVDTVLGDTERLLGVAPQRFSDWVRRWHALA
                     TQAELLFDIATTFAGERGDAAESEVLLWAIEFKANVASGVRDTEPLVLAMNASGSHCL
                     PGVPSTASAIRFSSRAPRCATCPTTAAR"
BASE COUNT         1457 a         2923 c         2834 g         1337 t
ORIGIN      
        1 taccgaccgc cactgcaact gcacctacgc tgctcgcatc aattggagtc cgagccgaac
       61 ggcgggacgg ccatttccag cgagttcacc aacgaaggga aacaccatga actgggaggt
      121 catcgaaggc aactggaagc agttcaaagg gcatgtgaag gagaaatggg gcaagctcac
      181 cgacgacaat ctcgaccgga tcgccggcaa gcgcgaccag ttggcgggaa gaatccagga
      241 aacctacggc atcaccaagg atcaggccga gcagcaactg aaggcctttg aacaggttca
      301 caaggactat cagccgaaga attcgacgta gctgcgaccc tttatgttcg gctggccgga
      361 tcgtccggtt tctccggcct tcctcaaagg attcgcggcc gtcgcgaccc gaaggatgag
      421 ggcctggagt tcgagatcgt cgtgactcgg gaggtcttca ccggcggcgg ctacatggcg
      481 cagccacgtc gaccctggtc gctttgcgcg cgtcagcggc gcccgcacac acgggcacaa
      541 tctcgccaat gacgaaccgc attgtgttcc tggtgccggg cttctttggc ttcacgtccg
      601 tcggcgccgt gagttatttc gaggatgtgg agcaggtact tggccgcgcg cttcgtcgac
      661 gcggggtcga ggctcgaatc gtacgttgca taacgcagcc gacggcatcg atcccacggc
      721 gcacccaggc gctgcgccgc caggtattcg acgccggcgg cctgaaagcc cgcgagcttc
      781 atttcgtcgg ccactcgacc ggcggtctcg acgtgcgcat gctgctgacg cccggagtga
      841 agatcgcagg cgatggttcg gaagaacgga ttgcccgcct gacgcgcacg tgcatctccg
      901 tggccacgcc gcaccatggc acgccgctcg cgaatcactt cgccagcgtc cagggccaga
      961 ccctgctgct ggtgctcacc gcccttgcga catccggaca gggacgcggc gcgattctcg
     1021 cggcggcaaa ggcgatcgcg atggtcgccc gtatcgatga ctggctggga cgcaaggaca
     1081 cctcactcga tcgcatcgcg gagcgcctgc tcaggaagat gagattcgat cgccgcgacc
     1141 cggtgtggaa atacctcggc gagatcgagc gcgatcaggg cgccgtgctg cagctcacgc
     1201 ccgagggcat cgagctgttc gatgccgccg tcgcggatcg tgccgggatc gattacggct
     1261 gcgtcgttgc gggcgtgccc aagccgcgcg agaggttcaa gctgaaggag ctgctggatc
     1321 ccgagtacgt cgcactgcgc atgttgttcc ggctgctgca cgcactgaca gcgcaggcgc
     1381 acccgcgcta cccctatccc caaatcacca agccgacgca gcgtgtgctc gatcgagggc
     1441 tcggcttcga ggcaacgccg tcgatcagcg acgggatcgt gccgaccctg agccagttgc
     1501 atggactggt gctgcacgtc gcgcgcgccg atcacctcga tgtcgtcggg cactacacac
     1561 tggcgggcaa gggcagcggc gactggctgc cctccggcgc cggcttcacg aaggaagcat
     1621 tcgacgccac ctgggaggcc gtcgctgcag cgatcgcgag gcgtcgccga ccggctcgcg
     1681 ccgactgaca acagtgcgca gttttcaggg aatttcgact tccgtgtagc ttcccgccgt
     1741 ggcgccgtcg accgccgcca cgactgcact tgcggcgcgc cgcattctcg gccaactggt
     1801 cgtgccgagc acgacgatgg cgatctgcct cgatgcgaga ttctgctgat acttgagatt
     1861 cgcgtcagtg gtgaccagaa cggcgaagcc ttccttctcg gcaaggtcga gcaagtctcc
     1921 attcttgagc tttgaccagc cgcgctcgta ggcggtcagc acttcgtgct gcgtcaatag
     1981 ctgccgcaga ggaacggggg ttccctggtc gaagaggacg cgcactcagg ctgttgccag
     2041 cgcactccgc gcggcgtgct ccagcacggc gcgaacttgt tcgatggtga cgcctgggaa
     2101 ccagttgacg aactccgaca cgagtgcccc gtcctcaagg ttctcaaaca gcgctgccac
     2161 gggcaccctg gtgccgcgga aaacccagac gccgctcacg cgctccgggt cgcgctcaac
     2221 ggctgcacac gtggaccaat cgatcatgtc tcgcatcgta ctaccgctac agcaaaggga
     2281 ggcggccaac ggcccggccc aagagtgcat aggaatagcc gacgcgatcg gctggcgcga
     2341 accgtcgtca caccgtggcc gatcgaagca acagcgcaga gcgacctcgc aacgggctca
     2401 ccccagcacg acgtcgacgc ggtgcgtctg tccatcgtcg cgcagcgcga tgccgtgctc
     2461 gggacgttcg agccgctcgc cgtcgagttc gatcgtcgcg acgccgcgcg cgacgccgcg
     2521 cggattttcc acgctgactt cgtagtcggt ggcgccgtgg cgatagcgga ccttgaaacc
     2581 ggaccagtgc ggcgggatgc acggcgcgac atgaagcaca tcgccgcgtt tcgtaatacc
     2641 caacatggat tcggtcccgg cgcgatacag ccatcccgcc gcgcccgtgt accaggtcca
     2701 gccgccgcgc ctgacatgcg gcggctcggc gtagatgtcg gctgccacga catacggttc
     2761 gaccttgtac gcgtacacgc cggcgcggct cgccgcgcga ttcaccggat tgagcatcgc
     2821 gaacaactcg gccgcccgtt cgccgtctcc caaccgggcg cacgccacca cacaccacac
     2881 agccgcgtgc gtgtactgcc cgccgttctc gcgcacgccc ggcaggtagc ccttgatgta
     2941 gccaggggcg ttttcggtct tgtcgaaggg cggcgcaaac agcagcacga gatcgtcgcc
     3001 gggccgcacc agatacgcgt cgaccgcctc catcgcgcgc cgggcccgct cgggttcagc
     3061 cgcgcctgac agcacgcccc agctctgcgc aatggagtcg attcggcatt ccgaattgcc
     3121 cgccgatccc aatggagttc catcatcgaa gtacgcgcgc cggtaccacg cgccgtccca
     3181 ggcctggctc tctgccgcgg cctgcaggct cgccgcatgc gcgcgccagc gcgcagccag
     3241 ttcggatgcg ccgcggctgt cggcgagcgt cgcgaacttc tgcagcgtcg cgaacaggaa
     3301 ccaggccagc cagacgcttt cgcccttgcc gagatgtccg acgcggttca tcccgtcgtt
     3361 ccagtcgccg ccggcgatca acggcagacc gtgggcaccg acgggcaggc tgcgatcgag
     3421 cgcgcgctcg cagtgttcga acagcgtgcc gatctgctgc gaaaccgtcg gcgtgaagta
     3481 cgcgtcgtgc tgaccgggct cgagccgttg accctcgaga aacggcagct gctcatcgag
     3541 cacggccgcg tcgccggtca ccgcgacgta atgagccgcg gcatagggca gccagaccag
     3601 atcgtccgca caacgggtgc gcacgccgcg cccggtcggc ggatgccacc agtgctgcac
     3661 gtcgccttcc acgaactgcc gcgcagcggc acgcagcagt tgcgctcgcg cggccgccgg
     3721 cagcgccagc gtcagcgcca tcgtgtcctg cagttggtcg cggaagccat acgcgccgcc
     3781 ggcctgatag aacgccgcac gcgcccatag ccggcagacg atcgtctggt actgcagcca
     3841 ccggttcagc atcaggtcca tcgagcggtc gggcgtttcg acctgcaccg tgcccaggat
     3901 gcggtcccag ttccttctga tctcggccag caccgcatcg acgtcgacag cgcgataccg
     3961 gagcaccagg ccgcgtgcag tctcgcgatc cgccgcctgt ccgagaagga acacgagact
     4021 cgctcgttgg ccaggcttca gctcgagcac cgtctgcagc gcgcagcagg gatcgaggcc
     4081 ggcaccggct cggttgccga gcgcgacctc tgccgtcagc gcagcgggtc gttccaggct
     4141 gccgtgacgg ccgaggaact cgcctcgatc gcaggtgtag ctcgtcggcg acccgccgag
     4201 atcgacaaac gcaacgcgcg cgccgaactc gtcgtgcagc gggttacggg cgaacacggc
     4261 gccggtggtc gcatcccgct cggtcacgac gtagggcgcg ctcttcgatc gcgtcgtccc
     4321 cagcacccat tcgacatagg cggtgatcga taaccgccgc ggccgtgagg agcgattgac
     4381 cagcgtgagg cgcgaaagct tgaccggatc gtcccagctc acgcactgca gccagtcgca
     4441 ctggatgccc gcgtagtcca attgaaaccg gctgtagccc tggccgtggc gtgcgacata
     4501 ggtcgcatgt tcgatgcgga tcggcgccgc tgtcggcgtc cacagttcgc cgctgtcctc
     4561 atcgcggaca taaaggacct cgccgggcgg atcggtgacc ggatcgttcg accacgcggt
     4621 caactggttt tcgcggctgt tctgcgacca ggtgtagccg gcgccggtct ccgatacctg
     4681 gaagccgaag tccggattgg cgaccacgtt gagccacggc atcggcgtgt gctggcgcgg
     4741 gccctgaacg atcacgtact cgcggcccgc gtcggcgaat ccgccgagac cgttgaagaa
     4801 ctcgagcggc ggcaactgcg gcgccggcgc atccgcgctg cggcgcgtga cctcgctccg
     4861 cttcgcaggt tcggcggcgg cattgaatcg ctgcaggcgc aggatttgct cggcgaggcc
     4921 gccctggctg ccgatcaaca cggcgcgcgc cactgtgtgc agcagcgcgc gctcgtcgcg
     4981 gccgagcagg tcggcgcgca gcacgaagac gcggcccagc ggaccctgcg cgtcctggcc
     5041 gatcgtcgcg cggctggagc gcgccatgtc ttccagcaac atctgcaggt cctgcaggta
     5101 ggagctcgcc ttgtcgttca gcagcaccag gtcgaccgcg agccccttca tctgccagta
     5161 ctcgtgtgcc cgcagcagtt gcgcgacgat gccgcgatcc tcgaccgcat cgatgcacag
     5221 caggacgatc gggtgatcgc cggagatgcc gtgcggccac agtccggcgg tgctcaaggt
     5281 gttgcgcttc agctcatcgc tcgccggccg caccgctggg tcggaaaaca ggatgcggct
     5341 gccgagctcc tggtacagag ccgcgtcgtc ggcctcgatg ccgagatggt gcaactgcac
     5401 ctgcgcatgg gtgaaggcca gcgtcgaaat gcgctcgaag gtcgaggcgt cgtgatactt
     5461 gtcggcgaga tcgaggaccg cgtcgcgcga ggcggcgacc atcgtcgaga agaccgtgtg
     5521 cacggtcgcg cccggcgcca gacgcacgcg tgtgcgcagg ctcagcaccg gatccagcac
     5581 cggtcccacg gtgttcgaca gcgggtggcc atcgagcacc gccgctggcg tccggaccgt
     5641 gcgcccgcgc cccagaaacc gggcgcggtc ggtctcgtat tcgaggccgt ccgcgttggc
     5701 gcggtcgcgc gtcacgacgt gagcgaccca cagcgcgggc tcttctggcg agcgggtacg
     5761 gcgcgtggcg agcaggccgc gcacttcggg cacgaactcg gtctgcacga acagattcga
     5821 gaacgccgga tgcgcgatgt cggccgccgg ctcggtcagc acgacttcgg cataggaggt
     5881 gatctcgacc tcgcgcgcgc gcgacccgtt gttggtgatc gacaggcggc ggacctcggc
     5941 gtcgtcttcg gacgagacga tgacttcgag caccgtcgtc aatgcgccgt cgcgccgcgt
     6001 gaagcgcgcg cgatcctcgg cgaatacgac cctgtagtca tcgggctcga ccgcgccggg
     6061 ctgatacgtg gccgaccaca gcttgccgct gtccacgtcg cgcaggtaca ggtacgagcc
     6121 ccagccgtcg gtggttgcgt cttcgcgcca gcggctcacg acgagcttgc cgcgctggct
     6181 gtagcctgag ccggccgctg tcatcatcac ggtgtagcgg ccattggaca acagatgcgt
     6241 cgcaggaatc ggcgtgtgcg cagtgtcgaa ctgacgcatc gccgggcggg ccgcctctct
     6301 gaccggcgtg gccggcaggt gctcgagcct tggacggtgg ttggccacct cgcgcggcgg
     6361 ccgctcctgc agcaacagct cggccgcgtg cgcgagggga tcgccatgga agcggtggcg
     6421 catgatgccg tcgcgcagcg aattgcccag cgccaccagc gagattccct ggtggtgcgc
     6481 catccaggca cgcacgacgg ccgcgctctg accgtcggcg agccgcgccg gcgtgtagtc
     6541 gatcgcctcg aacggcccgt agcggcccac gcccccttcc gtctgcaggc gcaggagatt
     6601 gcgcgccgca gccggcgcat cgaacatcgc tgccatcgcg gtcgcgtaag gagcgaccac
     6661 gagatcctcg cccagacccc gcttcaggcc caggccgggc acaccgaaat cggaatactg
     6721 ataggtgagc gcgcggtcgc gctggttgta ggcggactcg gagatgcccc aggggatcga
     6781 attctcattt ccatattcga tctgccgtgc gaccacgccg cggcacgttt ggtcgagcag
     6841 cgtgccgtgc ggcgtgtcca gcaccagcga tggcatcaga tactcgaaca tcgaccccga
     6901 ccacgacaac agtaccgggc gccggtgcac ggtcgtcgcg gcccggccca gatggaacca
     6961 gtgcgtcgac gcaacgtcgc ccttggcgat ggcgacaaaa ctcgcgagcc gggcctcgga
     7021 ggcgagcagg tcgtagaagc tgtcatcggg tgccgcagcg tcgacgcgat agccgatcgg
     7081 gaacagctgc cggttcacat cgaagagaaa cgcgaagtcc atctccgcaa cgaggcggcg
     7141 cgcgcggtcg gccagccgcg ccatctgcat cgtgtgatcc gcacaggccg cgccccagcg
     7201 cgcaacagcc aggatggtcg cttccggcgc catgccttcg cgcagccctg caacgatcgc
     7261 ggggaccgac atcccgacgt cgcccggcga cgcatcgacg cgcagatgcc ggtcgagcaa
     7321 ttcaagcagc agcgcgtgtg ctgcacccgg tgtcgaaccc atcgccggca aacgcttcat
     7381 cgccagcact agcggctcga tgtcgcgcac accgctcgcg acattcgccc ggaactcgat
     7441 cgcccacagg agcacttcgc tctcgtcggc atcgccgcgc tcgccggcga aggtcgtcgc
     7501 gatgtcgaac agcagttcgg cttgcgcctc cagcgcgtgc cagcgccgga cccagtcgga
     7561 gaagcgctgc ggcgcgacgc tcagcaatcg ctcggtgtct cccaggaccg tttcgacctg
     7621 accgcgattg acgatcagcg tgcggcggtc gtcgggcatg tcgcgcagcg cggcgcgcgc
     7681 gagctgaagc gaatcgccga ggccgtcgag ggcactccgg gcaaacagtg gctgccggat
     7741 gcattcatcg caagcaccag cggctcggtg tcgcgcacac cgctcgcgac attcgccttg
     7801 aactcgatcg cccacagcag cacttcgctc tcggcggcat ccccgcgttc accggcgaag
     7861 gtcgtcgcga tgtcgaacag cagttcggct tgcgtcgcca gcgcgtgcca gcgccggacc
     7921 cagtcggaga agcgctgtgg cgcgacgccc agcaatcgct cggtgtctcc caggaccgtg
     7981 tcgacctgac cgcgattgac gatcagcgtg cggcggtcgt cgggcatgtc gcgcagggcg
     8041 gcgcgcgcga gctgaagcgc atcgccgagg ccgtcgaggg cattccgggc aaacagtggc
     8101 tgccggatgc attcatcgca accctgggcc acggcgagca gatgcccggc cagattgccg
     8161 ctgtccacgg tggaaacgta tttcggctcg agcggccgca gctcgcgcgt ctcgtaccag
     8221 ttgaagaagt gcccgcgata ccgcggcagc cgaagcaacg tgtccgacgt cgcttcgatc
     8281 cggtcggcca gctcggccag gccgatccag ccgaagtcgc gcgcggcgac gcacgacagc
     8341 aggtacagcc cgaagttggt cggcgagctg cgatgggcga cgaccggttc gggcgtctgc
     8401 tggaagttgt cgggcggcag cgcatgatcc tcggccgtaa cgaacttggt gaagaagagc
     8461 cagatgcggc gcgccgtgcc gcgcagcagc gccacttgcg cagccgacaa cggctcggct
     8521 gcagcccgcc tggggggcag gctgatgaag c
//
LOCUS       DBNHBY010000298         1652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1506751, whole genome shotgun sequence.
ACCESSION   DBNHBY010000298 DBNHBY010000000
VERSION     DBNHBY010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1506751"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1652)
                     /gene="hrpB"
                     /locus_tag="AC16HB_07320"
     CDS             complement(<1..>1652)
                     /gene="hrpB"
                     /locus_tag="AC16HB_07320"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HIR5606639.1"
                     /translation="EGILTRRLQHDPALEDVGAVLFDEFHERSLQSDLGLALALDAQR
                     HVRPELRLLVMSATLDGAAVAALLGAGTPVVRSAGRAYEVVTHYAPPPAATATTRVRP
                     EPVEQRAARLVVRALEAHAEGDVLVFLPGAAEIRRACAAISDASAAAGDASSQLRVVP
                     LYGELDARAQDEALRPAPAGTRKVIVATNVAETSLTIEGVRIVVDAGLERRSRFDPNT
                     GMSRLATVAISRASADQRRGRAGRTAPGVCYRLWSESAHSALAAQAPPEILEADLAPV
                     ALELACWGAEPEALAWLDPPPAPMLEQARDLLRRLEALDARGQPTAAGRRMAALGTHP
                     RLAHMIERALPLRLTSLACAIAALLMERDPLRVEAAVTQRDPDLRHALDVLDGAPPPP
                     GHVVDARALQAVRRSAELLERRVAQRSDVRGAHHDSSTPPAPGRDAAAGLLLAFAYPD
                     RIGRARSAESGRYLLSGGRGAVLPASTALARSEYIVAAELDAGEREARILRAAPLERT
                     LLEAHFKPAIVTRDEIHWDERASAVVARRVRELDALVLDERP"
BASE COUNT          223 a          598 c          619 g          212 t
ORIGIN      
        1 ggggacgctc gtcgagcacg agcgcgtcga gttcccgcac tcgacgcgcg acgacggcgg
       61 aggcgcgttc gtcccagtgg atttcgtcgc gcgtcacgat ggcaggcttg aagtgggcct
      121 cgagcagcgt gcgctcgagc ggcgcggcac gcaggattcg cgcttcgcgc tcgccggcgt
      181 cgagctcggc cgcgacgatg tactcgctgc gcgcgagcgc cgtggacgcc ggcagcacag
      241 cgccccgtcc accgctcagc aggtagcgtc ccgattcagc ggagcgggcg cgcccgatgc
      301 gatccgggta ggcgaacgcc agcagcaggc cggccgcagc gtcacgcccg ggcgctggcg
      361 gcgtgctgct gtcgtgatgt gcacctcgga catcgctgcg ctgtgctacg cgccgctcca
      421 gcagttctgc ggaacgccgc acggcctgca acgcccgcgc atcgacgaca tggcccggcg
      481 gcggtggcgc gccatccagc acgtcgagcg catggcgcag gtccgggtcg cgctgcgtaa
      541 cggcggcttc gacccgcagc gggtcacgct ccatcagcag cgcggcgatg gcacaggcga
      601 gcgacgtcag gcgcagcggc agcgcgcgtt cgatcatgtg cgcgagccgc ggatgcgtgc
      661 cgagcgcagc catgcgacgg cctgcggccg tgggctgccc gcgtgcgtcg agtgcttcga
      721 ggcgtcgcag caggtcgcgc gcctgctcca gcatgggtgc gggcggcgga tcgagccacg
      781 ccagcgcctc cggctcggcg ccccagcagg ccagctcgag cgcgaccgga gcgaggtcgg
      841 cttcgaggat ttccggcggc gcctgtgcgg ccagcgccga gtgagcggac tccgaccaca
      901 gccgatagca gacgccgggc gcggtgcgtc cggcacggcc gcggcgctgg tcggcggaag
      961 cgcgcgagat cgcgaccgtc gccagccggc tcatcccggt gttcggatcg aagcgagagc
     1021 gacgctcgag gcccgcgtcg acgacgatgc gcaccccttc gatggtaagg ctggtctccg
     1081 cgacgttcgt cgcgacgatg accttgcgcg tgcccgcagg cgcaggacgc aaggcttcgt
     1141 cctgcgcgcg tgcgtcgagt tcgccgtaca gcggcacgac gcgcagctgg ctggaggcgt
     1201 ctcccgccgc cgccgaagcg tcgctgatcg ccgcgcaggc acgccggatc tcggcagcgc
     1261 ccggcaggaa caccagcacg tctccttcgg cgtgggcctc cagcgcgcgc accaccagtc
     1321 gcgccgctcg ttgttcgacc ggctccggac ggacgcgcgt cgtcgccgtg gccgccggcg
     1381 gcggggcata gtgcgtgacg acctcgtagg cgcgacccgc cgagcgcacg accggcgtgc
     1441 cggcgccgag cagcgcggcg acggcggcgc cgtcgagcgt cgcggacatc acgagcaggc
     1501 gcagttccgg ccgcacgtgg cgctgcgcat cgagcgccag tgccaggccc aggtcggact
     1561 gcaggctgcg ctcgtggaac tcgtcgaaca gcacggcacc gacgtcttcc agcgccggat
     1621 cgtgctgcag ccgtcgcgtc aggatgccct cg
//
LOCUS       DBNHBY010000299         7370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_965368, whole genome shotgun sequence.
ACCESSION   DBNHBY010000299 DBNHBY010000000
VERSION     DBNHBY010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_965368"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="trkA"
                     /locus_tag="AC16HB_07325"
     CDS             complement(<1..1403)
                     /gene="trkA"
                     /locus_tag="AC16HB_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR5606640.1"
                     /translation="MKIIILGAGRVGGSVAESLVSEANDITMVDTDAARVADLQQRFD
                     LRGLVGNATLPSVMREAGAADADMLIAVTSADEINLVACKIAADLFNVPTRIARVRNV
                     ELQKHPQLTGEQGFRATHIIWPEQSVTDYILKLIEFPEALQVLEFADGLVSLIAVRAV
                     AGGTLVSHPIRDLRRHIPNVDTRIVAIFRKDHAIQCEGTTMIEPGDEVFFLSASNDIR
                     TVMSELRRMDKPVKRVMIAGGGNIGLRLARALDTGEGIDWGAGFNVKIIEFSRVRCEY
                     LAAEVSSRVLVLNGDATDEDLLQDEGVAETDLFAALTNDDENNIMSALLAKRLGARRT
                     IALINRKSYGDLMQGGQIDIAISPSHATLLALLKHVRRGDVVAAHNLRRGAAEALEII
                     AHGDARSSRVVGRKIEQIELPKGTLIAAIVRGQSDTARVLIAHHDTVIESDDHLILFV
                     ASKRMIPRIEKLFAVGVG"
     gene            complement(1521..2204)
                     /locus_tag="AC16HB_07330"
                     /pseudo
     CDS             complement(1521..2204)
                     /locus_tag="AC16HB_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2284..4551)
                     /locus_tag="AC16HB_07335"
     CDS             complement(2284..4551)
                     /locus_tag="AC16HB_07335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5606641.1"
                     /translation="MSRALRYGTIASLALGGVLLFLLASASSNVQAFERHYPLLLAIN
                     GAVAVLLFIFVVALVLRVVRRWRARRFGARLMARFALAFALMGVVPGVLIYVVSVQFL
                     SRTVESWFDVRVDRALESGLSLGRAALEAQLADVSAKARVIALELADATASTQLAVLD
                     RARERAGIQEAVLLSGNGQVIGTAGGRPTELAPELPTAAQVRQARQSRTFAIIEGDPL
                     AVDPRSGLRTRVIVPIPSSANAARALDFSLAATSPVGEQRFLQLMQRVPASVAANAEA
                     LQSGYRDYQELSLSRAGLQRIYTVTLTLTLLLAIFAAVASGVLLASSMTAPLLQVAEG
                     TKAVAEGDFRPVREFAGNDELNLLTQSFNAMTRQLAEARDAVESRSRELENARAFLER
                     VLANLSAGVVVLDQDFRLVTANYGASRILGLPLAPRAGARFDAVVPALAEQIAAAFGE
                     QALAAAPSESWQRQIELARSEADGAREAQALLVRGSRLPLDNGPGYVVVFDDITQVIS
                     AQRAVAWGEVARRLAHEIKNPLTPIQLAAERLQMKLADKLPPDQAAVLSRGAATIVGQ
                     VAAMKRMVDDFRRYARVPPAELAALDLNALIEEVAQLYGADGMIELELARELPPIEGD
                     ATQLRQVIHNLIVNAQDALAQPDLGHAARIVLRTEAVPVREPGAAPSAAVRLTVEDNG
                     PGFAAHILKRAFEPYVTSKPSGTGLGLAMVKKIVDEHGARIELVNRSGGNAGASVIIV
                     FRRLAAAAGAQRRVA"
     gene            complement(4570..5199)
                     /locus_tag="AC16HB_07340"
     CDS             complement(4570..5199)
                     /locus_tag="AC16HB_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR5606642.1"
                     /translation="MIRAACRIDDCAAATRIAVAARALLAAAALALAAWTAPSVASDR
                     IVVSGAELQLAADADGGVVLNAQFDFELPPVLEDAVNRGIALYFVIEFELYRERWYWF
                     DRRIASQSLTYRLTYSPLTRQYRLARDALAQPFDSLAEAMATLRRVRNWKVLERGVVK
                     PDEDYRAQVRMRLDTSQLPRPFQVNALTQRDWTLASDWRNVGVTADLGR"
     gene            complement(5208..6554)
                     /gene="rsmB"
                     /locus_tag="AC16HB_07345"
     CDS             complement(5208..6554)
                     /gene="rsmB"
                     /locus_tag="AC16HB_07345"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR5606643.1"
                     /translation="MNGAPTAPPLADALRVAAFAWARLRAGTSLERALEAALQAVAAP
                     EHPRLAAAAKDIAYTATRHLALIDAAIARLATRMPDAAVGALVAVALGQLLSARRAEY
                     VVVDQSVRAAKADPATRAAGGFINALLRRWLREREARLPELLRDDGVRFNAPTWWIDR
                     VRAAHPQRADALLALQRQPPPLVLRVNLRRTSVEDYLARVARDGIAAEQVGATAVRMN
                     APRPVEAIPGFAQGEVSVQDAGAQLAGQLLAARDGMRVLDACAAPGGKTAQLAELADV
                     ELDAVELDAARAARIESNLSRTAAQEHAHIRVIVADATRPQSFAPVGGYDRILLDAPC
                     TASGIVRRHPDIPWLRRPADVAQLATLQSRLLDALWPLVSRAGRLLYVVCSLFPEEGE
                     RQARQFAARRADARVVPLAAGAGPFLQVLPAEAAHAWRGGLPTVHDGFFFALFEKS"
     gene            complement(6551..>7370)
                     /gene="fmt"
                     /locus_tag="AC16HB_07350"
     CDS             complement(6551..>7370)
                     /gene="fmt"
                     /locus_tag="AC16HB_07350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5606644.1"
                     /translation="ATPPTLSLKKGGNDAASAHAQLRAVAADVLVVAAYGQILPQAVL
                     DTPRGLPDAGAGRVTAINIHASLLPRWRGAAPVARAIEAGDRETGITIMQMDAGLDTG
                     PMLLTERIAIGDGETTGTLTARLAEVGARLIVRALQAGGRGELRALPQSGTGVTYAHK
                     IEKREAWLDWTHDAETLARRVRAFDPFPVACGEVRGTTLKLWRAHALAGDVARVPGTV
                     VAADADGIRIACGTGMLVVMELQRPGGRRMSAAEFLAGMPLAAGDAFRAASASA"
BASE COUNT         1109 a         2664 c         2519 g         1078 t
ORIGIN      
        1 ccgacgccga ccgcgaacag cttctcgatg cgcgggatca tgcgcttgct ggcgacgaac
       61 aggatcaggt ggtcgtcgga ctcgatgacc gtgtcgtggt gcgcgatcag cacgcgcgcg
      121 gtgtcactct gcccgcgcac gatggccgca atcaaggttc ctttcggcag ttcgatctgc
      181 tcgatcttcc gcccgaccac gcgcgagctg cgcgcgtcgc cgtgcgcgat gatctcgagc
      241 gcctccgcgg cgccgcggcg caggttgtgc gccgccacga cgtcgccgcg ccgcacgtgc
      301 ttcagcagcg ccaacagcgt cgcgtggctc ggcgaaatgg caatgtcgat ctggccgccc
      361 tgcatcaggt cgccgtagct cttgcgattg atcagcgcga tcgtgcgccg cgcgcccagc
      421 cgtttcgcca gcagcgcgct catgatgttg ttttcgtcgt cgttcgtgag cgccgcgaag
      481 aggtccgttt ccgccacccc ttcgtcctgc agcaggtcct cgtcggtggc gtcgccgttt
      541 agcaccagca cgcgcgacga cacctcggcc gccaggtatt cgcagcgaac cctgctgaac
      601 tcgatgatct tgacgttgaa gccggcaccc cagtcgatgc cttcgccagt gtcgagcgcg
      661 cgcgccagcc gcagcccgat gttgccgccg ccggcgatca tcacgcgctt gaccggcttg
      721 tccatccggc gcagttcgct catcacggtg cggatgtcgt tcgacgcgga caggaagaac
      781 acctcgtcgc cgggctcgat catcgtcgtg ccttcgcact ggatcgcgtg gtccttgcgg
      841 aagatcgcca cgatgcgcgt gtccacgttc gggatgtggc gccgcaggtc gcggatcggg
      901 tgcgacacca gcgtcccgcc agccaccgcg cgcaccgcga tcagcgacac cagcccgtcg
      961 gcgaattcca gcacctgcag cgcctcggga aactcgatca gcttcaggat gtagtcggtg
     1021 acgctctgct ccggccagat gatgtgggtg gcacggaagc cctgctcgcc ggtgagctgc
     1081 gggtgcttct gcagttcgac attgcgcaca cgcgcgatcc gcgtcggtac attgaacagg
     1141 tcggcggcga tcttgcacgc gaccaggttg atctcgtcgg ccgaagtgac cgcgatcagc
     1201 atgtcggcgt cggccgcgcc ggcctcgcgc atcaccgacg gcaaagtggc gttgccgacc
     1261 agcccgcgca ggtcgaagcg ctgctgcaga tccgccacgc gcgcagcgtc ggtgtcgacc
     1321 atcgtgatgt cgttggcctc ggacacgagg ctttcggcca ccgagccgcc gacccgcccg
     1381 gccccgagga tgatgatctt cacactgcgc cgcgcgcgcg gctactccac ttcacggtcg
     1441 ggagcggcct gcgtcgcagc ctcgggcggc gcgtgactcg cgagcgcggg cgcgccgccg
     1501 cgctcgctgc gtcgactgcc gcgcgccagt tcgatgccga gctgcttgag tttgcggtac
     1561 aggtgcgtgc gttccagccc ggcacggtcg gccacgcgcg tcatgctgta gtgctcgcgc
     1621 tccaggtggt actcgaagta cacgcgctcg aaatggtcgc gcgcctcgcg cagcggctgg
     1681 tcgaggctca ccgtactggc gttcaccgcg gccgatgccg tcgcgcgtac ctgcccgcgc
     1741 tcgggcggcg caatcggcgc cacggcgggc gcggccggcg gctcgatcgg ctgcgccggt
     1801 gtcgccacga tcggaccggc acgcaggttg cgcataagtc ggccgcgatc cagaccgccc
     1861 ttcaccgcgc cgagcaggcg cgtcagcgcg atcggtttct cgagaaagtc gagcgcgccg
     1921 aagcgggtcg cctccaccgc cgtgtcgatt gtcgcgtggc cggacatcat gatgaccggc
     1981 atcgtcagct gccccgacga cgcccactcc ttcagcagcg tcacgccgtc ggtgtcgggc
     2041 atccagatgt cgagcagcac gaggtcgggc gactcggaca ggcgcagcgc acgcgcgacc
     2101 gacgcgttct cggccgtgaa cacgacgtgt ccttcgtcgg acaggatctc cgacagcagt
     2161 tcgcggattc cgacttcgtc gtcgacgacc aggatcgtgg acatacgaat tacgcggccc
     2221 ggggcgagat tgaggaacgc atgtcggtac agatgctcat cgtttcagga tcgtgtcggg
     2281 gactcaggcc acgcgacgct gcgcgcccgc cgcggcggcc aggcgacgga agacgatgat
     2341 caccgacgcg ccggcgttgc cgccgctgcg attgacgagt tcgatgcggg cgccatgctc
     2401 atccacgatc ttcttcacca tcgccaggcc caggccggtg ccgctcggct tgctggtcac
     2461 gtaaggctcg aaagcgcgct taagtatatg cgcggcgaag cccggtccgt tgtcctccac
     2521 cgtcaggcgc accgccgcgc tcggtgccgc gccgggttcg cgtaccggca ctgcctcggt
     2581 gcgcaggacg atgcgcgccg cgtgaccgag gtccggctgc gccagcgcgt cctgcgcgtt
     2641 gacgatcagg ttgtggatca cctggcgcag ctgagtcgcg tcgccctcga tcggcggcag
     2701 ctcgcgcgcc agttcgagct cgatcatgcc gtcggcaccg tatagctgcg cgacttcctc
     2761 gatcagcgca ttcaggtcga gggcggcgag ctccgccggc ggcacgcgcg cgtacctgcg
     2821 gaagtcgtcc accatccgct tcatcgccgc gacctggcca acgatcgtgg ccgcgccgcg
     2881 cgacagtacc gcggcctggt ccggcggcag cttgtcggcg agcttcatct gcagccgttc
     2941 ggccgcaagc tggatgggtg tcagcgggtt cttgatctcg tgcgccagcc gccgcgcgac
     3001 ctcgccccac gccaccgcac gctgcgccga gatcacctgc gtgatgtcgt cgaacacgac
     3061 cacgtagccg gggccgttgt ccagcggcag cctggagccg cgcacgagca gcgcctgcgc
     3121 ttcgcgcgcg ccgtcggctt cgctgcgcgc cagctcgatc tgccgctgcc acgactcgct
     3181 cggcgccgcg gcgagcgcct gctccccgaa cgcggcagcg atctgctccg ccagcgccgg
     3241 gaccaccgcg tcgaagcgcg cgccggcgcg cggcgcgagc ggcagcccga gaatgcggct
     3301 cgcgccatag ttggcggtga ccagacggaa gtcctggtcg agcacgacca cgccggccga
     3361 caagttcgcc agcacgcgct cgaggaacgc gcgcgcgttc tcgagttcgc gcgaacgcga
     3421 ctccaccgcg tcgcgcgcct ccgccagctg ccgcgtcatt gcgttgaaag actgcgtcag
     3481 caggttcagc tcgtcgttgc cggcgaactc gcgcaccggc cggaagtcgc cttcggcgac
     3541 cgccttggtg ccttcggcca cctgcagcag cggcgcggtc atggagcttg cgagcagcac
     3601 gcccgaggca acggcggcga agatcgccag cagcagcgtc agggtcagcg tgacggtgta
     3661 gatgcgctgc agccccgcgc gcgacagcga cagctcctgg tagtcgcggt agccgctctg
     3721 cagcgcctcg gcgttcgcgg ccacgctggc gggcacgcgc tgcatcagtt gcagaaagcg
     3781 ctgctcgccc accggcgacg tggcagcgag cgaaaagtcg agcgcgcgcg ccgcgttggc
     3841 cgacgacggg atcggcacga tcacacgcgt gcgcagaccg gaacgcgggt cgacggcgag
     3901 cggatcgcct tcgatgatgg cgaaggtgcg gctctggcgc gcctgccgga cctgcgccgc
     3961 cgtcggcagc tcgggcgcca gttcggtcgg ccggccgccg gcggtgccga tcacctggcc
     4021 gttgccggac agcagtaccg cttcctggat gccggcgcgt tcacgcgcgc ggtcgagcac
     4081 cgccagctgc gtcgacgcgg tcgcgtcggc cagttcgagc gcgatcacgc gcgccttggc
     4141 gctcacgtcg gcgagctgcg cctccagcgc ggcgcggccg agcgacaggc ccgattccag
     4201 cgcgcgatcg acacgcacgt cgaaccacga ctccaccgtg cgcgacagga actgcaccga
     4261 cacgacatag atcagcacgc cgggcacgac gcccatcagc gcgaacgcca gcgcgaagcg
     4321 tgccatcagg cgcgcgccaa aacggcgcgc gcgccagcgc cgcaccaccc gcagcaccag
     4381 cgcgacgacg aagatgaaca gcagcaccgc gaccgcgccg ttgattgcca gcagcagcgg
     4441 atagtggcgc tcgaatgcct ggacgttgct gctggccgag gcgagcagga acagcagcac
     4501 gccgcccagt gcgagcgagg caatggttcc gtacctcagg gcgcgcgaca tgaatcgacg
     4561 aacggggcgt cagcgcccca gatccgcggt gacgccgaca ttgcgccagt ccgacgccag
     4621 cgtccagtcg cgctgggtca gcgcgttgac ctggaacggc cgcggcagct gcgaggtatc
     4681 gagccgcatc cgcacctggg cgcgatagtc ctcgtcgggc ttgaccacgc ctcgttcgag
     4741 caccttccag ttgcgcacgc ggcgcagcgt cgccatcgcc tccgccagcg agtcgaacgg
     4801 ctgcgccagc gcatcgcgcg cgagccgata ctgccgtgtc agcgggctgt aggtcagccg
     4861 ataggtcagt gactgggacg cgatcctgcg gtcgaaccag taccagcgct cgcggtacag
     4921 ctcgaactcg atcacgaagt acagcgcgat gccgcggttg accgcgtctt cgagcaccgg
     4981 cggcagctcg aaatcgaact gcgcgttgag caccacgccg ccgtcggcgt cggctgccaa
     5041 ctgcagttcg gcgcccgaca cgacgatgcg gtcggacgcg accgacggtg ctgtccacgc
     5101 cgcgagcgcc agcgccgcgg ccgcgagcag cgcgcgcgcc gccaccgcga tccgcgtcgc
     5161 cgcggcgcag tcatcgatac gacaggccgc gcggatcatc cgtggactca gctcttttcg
     5221 aacagcgcga agaaaaaccc gtcgtgcacg gtcggcagcc cgccgcgcca cgcgtgcgca
     5281 gcctcggccg gcagcacctg aagaaaagga cccgcgccgg cagcgagcgg cactacgcga
     5341 gcatcggccc ggcgtgccgc gaattgccgg gcctggcgtt cgccttcctc ggggaacagc
     5401 gagcacacga cgtaaagcaa tctaccagcg cgcgatacga gcggccataa tgcatcgagc
     5461 aaacgcgact gcagcgttgc taattgcgca acatcggccg gtcggcgtag ccagggaatg
     5521 tcagggtggc ggcgcacgat ccccgacgcg gtacacgggg catcgagcag gatgcggtcg
     5581 tacccgccta cgggcgcgaa cgactgtggt cttgtcgcat cggcgacgat cacgcgaatg
     5641 tgggcatgct cttgcgccgc cgtgcgcgac aggttcgatt cgatgcgcgc cgcgcgcgct
     5701 gcgtcgagtt cgaccgcgtc gagttcgacg tcggccagct ccgccagttg cgcggtcttg
     5761 ccgccaggcg cggcgcaggc atcgagcacg cgcatgccat cgcgcgccgc gagcagttgc
     5821 ccggcaagct gcgcgccggc gtcctgcacc gatacctcgc cctgcgcaaa accgggaatc
     5881 gcctcgaccg gacgcggcgc attcatacgc accgcggtcg cgccgacttg ctcggccgca
     5941 atcccgtccc gcgcgacccg cgcgaggtag tcttcgaccg aggtacgacg caggttcacg
     6001 cgcagcacca gcggcggcgg ctgccgctgc agcgcaagca gcgcatcggc gcgctgcgga
     6061 tgcgcggcgc gaacgcggtc gatccaccac gtcggcgcgt tgaatcgcac cccgtcatcg
     6121 cgcagcaact cgggcagcct cgcttcacgc tcgcgtagcc agcgccgcag cagcgcgttg
     6181 atgaagccgc ccgccgcgcg ggtggctgga tccgccttgg ccgcgcgcac cgactgatcg
     6241 acgacgacgt attcggcgcg acgcgccgat agcagctggc cgagtgcgac ggcgacaagc
     6301 gcgccgaccg cagcgtcggg cattcgcgtc gccagccgcg cgatggccgc gtcgatcagc
     6361 gcgagatgac gggtcgcggt gtacgcgatg tccttggcgg ccgcggcaag ccgcggatgc
     6421 tccggcgccg ccaccgcctg cagcgcggcc tcgagcgcgc gttcgagcga tgtgccggcg
     6481 cgcagccgtg cccacgcaaa ggcggccacg cgcagcgcgt cggcgagcgg cggtgcagtc
     6541 ggagctccgt tcacgccgat gcggacgccg cacggaaggc gtcgcccgcc gccagcggca
     6601 tgccggcaag aaactcggcc gcgctcatac gccgtccgcc cggccgctgc agttccatga
     6661 cgacgagcat gcccgtgccg caggcgatcc gaatgccgtc ggcgtcggct gcaacgaccg
     6721 ttcccggcac gcgtgccacg tcgcccgcga gcgcgtgcgc gcgccacagc ttgagcgtcg
     6781 tgccgcgcac ctcgccacag gcgacgggaa acggatcgaa tgcgcgcacg cgccgcgcca
     6841 gggtctcagc gtcatgcgtc cagtcgagcc acgcctcgcg cttctcgatc ttgtgcgcgt
     6901 acgtgacacc cgtgcccgac tgcggcagcg cgcgcagttc gccgcgcccg cccgcctgca
     6961 gtgcgcgcac gatcaggcgc gcgccgacct ccgccagccg tgcggtcagc gtgccggtcg
     7021 tctcgccgtc tccgatcgcg atccgttcgg tgagcagcat cgggcccgtg tcgagtccgg
     7081 catccatctg catgatcgtg atgccggtct cgcggtcgcc cgcctcgatc gcgcgtgcca
     7141 ccggcgcagc gccccgccag cgcggcaaca gcgacgcatg gatattgatc gccgtgacgc
     7201 gtccggcgcc ggcgtcgggc agtccgcgcg gcgtgtcgag gacggcctgc ggcaggatct
     7261 gcccgtaggc ggcgacgacc agcacgtcgg ccgcgacggc gcgcaactgg gcgtgcgcag
     7321 acgccgcgtc gttcccgccc ttcttcaggc tgagcgtggg cggtgtggcg
//
LOCUS       DBNHBY010000300         2578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_897465, whole genome shotgun sequence.
ACCESSION   DBNHBY010000300 DBNHBY010000000
VERSION     DBNHBY010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_897465"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..415)
                     /locus_tag="AC16HB_07355"
     CDS             complement(158..415)
                     /locus_tag="AC16HB_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTH_I0359 family protein"
                     /protein_id="HIR5606645.1"
                     /translation="MNMIYNSQLFCVVEFPSLGDEGSQAFGGFEIMDKQLRREIFLGG
                     KDAEEFRASVAKLISSEPTADDIDEFLGRYSGLMTTPVTLH"
     gene            complement(524..1327)
                     /gene="pcaD"
                     /locus_tag="AC16HB_07360"
     CDS             complement(524..1327)
                     /gene="pcaD"
                     /locus_tag="AC16HB_07360"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HIR5606646.1"
                     /translation="MAQVRANGIDIHYRIDGDGAPARPWLVFSHAIACDLTMWDPQVE
                     EFSRALNVLRFDTRGHGGSSAPPGDYTLETLADDVKALLDALGIQRCHFVGLSMGGMI
                     GQMAALRSPLRFASLTLVDTSSRYPPEMRSTWEQRIAAVMTPQGMNGVVPSTLERWFT
                     ARFRQMRPDVTAKVGALIRATPVAGYVGCARALSRLNLTARLGSIACPTLVIVGEEDH
                     TTPLAMAEDIVRAIPGARLERIADAAHLSSMEQPDRFNALLRAFLLRAA"
     gene            complement(1332..1685)
                     /locus_tag="AC16HB_07365"
     CDS             complement(1332..1685)
                     /locus_tag="AC16HB_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606647.1"
                     /translation="MNAVPVTLRVARDAVARQAALSDWLGADADVRCSPERAAVIVEG
                     ALTPLPAPAGLVVERVAGCFCCVGQVALGVTLTRLLRTRRPDRLLLLIASDEHLDRVR
                     RMLGDDRFAILRRVG"
     gene            complement(1682..>2578)
                     /locus_tag="AC16HB_07370"
     CDS             complement(1682..>2578)
                     /locus_tag="AC16HB_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR5606648.1"
                     /translation="IYVAISIVCMLAYGLAGMSWPDAFMHMCSTMGLGGFSSHDASFG
                     YWNSPLIEYTSVVFMLIAGFNFSMHFLAWRRRSLAVYWRDPEGKAFLGITLGASLLIA
                     LFLLWREQYADFWTAWRFALFNTVSIATTTGYASTDYNQWPIFAPALMLFLCCFATCA
                     GSTGGGIKMVRAIILVKQARREFVRILHPRIVNPVHLGGQVIDNNVISAVLAFMLIYG
                     GALIGFTLLLMASGLDPITSFTAIVASLNNTGPGLNQVGPATNYASLTDFETWVCTFA
                     MLIGRLELLSVLVLFTPAFWRK"
BASE COUNT          465 a          862 c          854 g          397 t
ORIGIN      
        1 cgatcgtcga cggcgtgcgc cagtacgacc tgcaggcgtt ccagtacgcg ctgctgttcc
       61 tgttcgcgtg gctggcggcg gccttcttac tgctgctcgt cacccgcgag acacacgccc
      121 gccagatgcc gtgacggacg tcgcgcgatg ctgcgattca gtgcagcgtg accggcgtcg
      181 tcatcaggcc cgagtaacgg cccaggaact cgtcgatgtc gtcggcggtc ggctcgctgg
      241 agatcagttt ggccacgctg gcgcggaact cttcggcgtc cttgccgccg aggaagatct
      301 cgcgccgcaa ctgcttgtcc atgatttcga agccgccaaa cgcctgtgat ccttcgtcgc
      361 cgagcgacgg gaattcgacc acgcagaaca actggctgtt gtagatcatg ttcatatcga
      421 ccctaacgct ttgccccgcc gcccggtgga cggaacctat ctatgccaaa tatcggtacg
      481 gccgccggtt tctcaagccc cgcggcggat ccggcgcggg atttcaggcg gcacgcagca
      541 gaaaggcgcg cagcagcgca ttgaatcggt cgggctgctc catgctcgac aggtgcgcgg
      601 cgtcggcgat gcgctcgagg cgcgcgccgg gaatcgcgcg cacgatgtcc tccgccatgg
      661 ccagcggggt cgtatggtct tcctcgccga cgatcaccag cgtcgggcag gcaatcgaac
      721 cgagccgtgc ggtcaagttc agccgcgaca gtgcgcgcgc acagccgaca tagcccgcca
      781 ccggcgtggc gcggatcaac gcgccgacct ttgcggtcac gtcggggcgc atctggcgga
      841 agcgtgcggt aaaccatcgc tcgagcgtcg acggcacgac cccgttcatg ccctgcggcg
      901 tcatgaccgc ggcgatccgc tgctcccagg tcgagcgcat ctcgggcggg tagcggctgg
      961 acgtgtcgac cagagtcagc gacgcgaagc gcagcggcga ccgcagcgcg gccatctggc
     1021 cgatcatccc gcccatcgag aggccgacga agtggcagcg ctggataccg agggcatcga
     1081 gcagcgcctt cacgtcgtcg gcgagcgttt cgagcgtgta gtcgccgggc ggcgcgctcg
     1141 aaccgccgtg gccgcgcgtg tcgaagcgca gcacgttcag cgcgcggctg aactcctcga
     1201 cctgcgggtc ccacatcgtg aggtcgcatg cgatcgcgtg gctgaacacg agccaaggcc
     1261 gcgccggggc accgtcgccg tcgatgcggt agtggatgtc gattccgttt gcgcgtacct
     1321 gtgccatgcc ttcagcccac ccttcgcagg atcgcgaacc ggtcgtcgcc caacatgcgc
     1381 cgcacccggt cgaggtgctc gtcgctcgca atcagcaaca acagccggtc cgggcgccgg
     1441 gtgcgcagca gccgggtcag cgtcacgccg agcgcgacct ggccgacgca gcagaaacag
     1501 ccggcgacgc gttccacgac gagccctgcg ggtgctggca gcggggtgag cgcgccctcc
     1561 acgatcacgg ccgcgcgttc cggtgagcat cgtacgtccg cgtccgcgcc cagccaatcg
     1621 ctcaacgccg cctgtcgcgc gacggcgtcg cgcgctactc gcagcgtgac cggcacggcg
     1681 ttcacttgcg ccagaaggcc ggcgtgaaca gcaccagcac cgacagcagt tcgagccggc
     1741 cgatcagcat ggcgaacgtg catacccagg tttcgaagtc ggtcagcgac gcgtaattgg
     1801 tcgccggccc cacctggttc agtcccggcc ccgtgttgtt aagcgacgcg acgatcgccg
     1861 tgaacgaggt gatcgggtcg agccccgagg ccatcagcag cagcgtgaag ccgatcagcg
     1921 cgccgccgta gatcagcatg aatgcgagca cggcggagat cacgttgttg tcgatcacct
     1981 gtccgcccag gtgcaccggg ttgacgatgc gcggatgcag gatgcgcacg aactcgcgcc
     2041 tggcctgctt gacgaggatg atcgcgcgca ccatcttgat cccgccgccg gtggatccgg
     2101 cgcaggtcgc aaagcaacag agaaacagca tcagcgcggg cgcgaagatc ggccactggt
     2161 tgtagtcggt gctggcatag ccggtcgtgg tcgcgatcga caccgtgttg aaaagcgcga
     2221 agcgccacgc ggtccagaag tcggcatact gctcgcgcca cagcaggaag agcgcgatca
     2281 gcaacgatgc gccgagcgtg atgccaagga acgccttgcc ctcggggtcg cgccagtaga
     2341 cggcaagcga acgccgccgc caggccagga agtgcatcga gaaattgaag ccggcgatca
     2401 gcatgaacac gaccgacgtg tactcgatca gcggcgagtt ccagtaaccg aagctcgcgt
     2461 cgtgcgagga aaagccgccc aatcccatcg tcgagcacat gtgcatgaac gcatccggcc
     2521 atgacatgcc ggccagcccg taggcgagca tgcagacgat cgagatcgcc acgtagat
//
LOCUS       DBNHBY010000301         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1303835, whole genome shotgun sequence.
ACCESSION   DBNHBY010000301 DBNHBY010000000
VERSION     DBNHBY010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1303835"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="AC16HB_07375"
     CDS             <1..617
                     /locus_tag="AC16HB_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5606649.1"
                     /translation="VWYAGGYKQALEDGRKPPQETRTIIYAPKGPVNFQPHHQPFNYY
                     ARFAPGTADRERHLKDYDDFVVAIDSGALPQVSFYKPAGRYNQHPGHTDVASGDVHID
                     ALLQRLRRSPQWKSMAIIVTYDENGGFWDHVPPPAGPGWSDRWGPASRVPTLVVSPFA
                     RRGYVDKTPYATTSIIKFITKRFGLVPLPGVRERAGDLTNAFEF"
     gene            complement(625..1170)
                     /locus_tag="AC16HB_07380"
     CDS             complement(625..1170)
                     /locus_tag="AC16HB_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5606650.1"
                     /translation="MTAVAGTARTDRLVEALAFAAHKHRDQRRKDVHASPYINHPIAL
                     AHVLAVEAGVEDEKTLIAAILHDTIEDTETTFEELRERFGLKVADIVAEVTDDKSLSK
                     QRRKELQIEHAPHLTRRAKLVKLADKICNLRDMAAHPPHDWPLARRREYFDWAKQVID
                     GLRGTQRRLEKLFDAAYADRP"
     gene            complement(1167..1748)
                     /locus_tag="AC16HB_07385"
     CDS             complement(1167..1748)
                     /locus_tag="AC16HB_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR5606651.1"
                     /translation="MSLLVLGLVLFLGVHSTRIVAGGWRAQQILHLGEQRWKGLYSLV
                     SLIGFVVLIYGYGLARQSPFVLYTPPIWARHVAALLTLPAFVLLAAAYVPGTRIRRAV
                     GGHPMLVGVKTWAVAHLLANGTLHDVVLFGAFLVWAVVDYASCRRRDRAEGRLYVAGP
                     VKRDATALIVGVLAWAAFAFWLHGWLIGVRPFG"
     gene            complement(1768..>2585)
                     /locus_tag="AC16HB_07390"
     CDS             complement(1768..>2585)
                     /locus_tag="AC16HB_07390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR5606652.1"
                     /translation="AVAARIDDADATEADAWWCAREVAQELKQAVRDATGLTCSIGLA
                     PNKLIAKMASELDKPNGLTVVTMDDLAVRIWPLPVRRINGVGPKSAAKLARLGIATIG
                     ELAAAAPDWLIAHFGASYGAWLCDAAHGRDERPVVTHSEPVSMSRETTFERDLHAVRD
                     RTALSRVFTALCEEVSDDLRRKHYAGRNVAIKLRFDDFRTVTRAITLPAPIADAQAIR
                     RAAGQCLKRVDLAKRIRLLGVRVGALSRAGDPDAHPAGAAELFDPPLGDTARS"
BASE COUNT          412 a          914 c          883 g          376 t
ORIGIN      
        1 gggtgtggta cgccggcggc tacaagcagg cactggaaga cggccgcaag ccgccgcagg
       61 aaacgcgcac gatcatctac gcgccgaaag gcccggtgaa cttccagccg catcaccagc
      121 cattcaacta ctacgcgcga ttcgcgcccg gcaccgcgga ccgcgagcgg cacttgaagg
      181 actacgacga tttcgtcgtg gcgatcgaca gcggcgcctt gccgcaggtg agcttctaca
      241 agccggccgg ccgctacaac cagcacccgg ggcacaccga cgtcgcctcc ggcgacgtgc
      301 acatcgacgc cttgctgcag cgactgcgcc gcagcccgca atggaaatcg atggcgatca
      361 tcgtcacgta cgatgaaaac ggcggcttct gggatcacgt gccgccgccc gccgggccgg
      421 gctggagcga ccgctgggga ccggcatccc gcgtgccgac gctcgtcgtc tccccgttcg
      481 cgcggcgcgg ctacgtcgac aagacgccgt acgccacgac ttcgatcatc aaattcatca
      541 cgaagcgatt cggactcgtg ccgctgcccg gcgtgcgcga gagagcgggc gacctgacga
      601 acgcgttcga gttctagcgg agcgctacgg ccgatcggcg taggccgcat cgaacagctt
      661 ttccaaccgg cgctgcgtgc cgcgcaggcc gtcgatgacc tgcttggccc agtcgaagta
      721 ctcgcgccgc cgcgcgagcg gccaatcatg cggcggatgg gcggccatgt cgcgcaggtt
      781 gcagatcttg tcggccagct tcaccagctt ggcgcgccgc gtcaggtgcg gcgcgtgctc
      841 gatctgcagt tccttgcgcc gctgcttgga cagcgacttg tcgtcggtga cctcggccac
      901 gatgtccgcg accttcaggc cgaagcgctc gcgcagttcc tcgaacgtcg tttccgtgtc
      961 ctcgatcgtg tcgtgcagga tcgccgcgat cagcgtcttc tcgtcctcga cgccggcctc
     1021 gaccgcgagc acgtgtgcga gcgcgatcgg gtgattgatg tacgggctcg cgtgcacgtc
     1081 cttgcgccgc tggtcgcggt gcttgtgtgc ggcgaacgcg agcgcttcga ccagccggtc
     1141 ggtgcgcgcc gtgccggcca ctgccgtcac ccgaacggcc gcacgccgat cagccagcca
     1201 tgcagccaga acgcgaacgc ggcccaggcg agcacgccga cgatcaatgc ggtggcgtcg
     1261 cgcttcacgg gcccggcgac gtacaagcgg ccttcggcgc ggtcgcggcg ccggcacgat
     1321 gcgtagtcga ccaccgccca cacgaggaac gcgccgaaca gcaccacatc gtgcagggtg
     1381 ccgttggcga gcaggtgcgc gaccgcccag gtcttgacgc cgacgagcat cgggtggccg
     1441 ccgaccgcgc gcctgatccg cgtgccgggc acgtacgcgg cggcgagcag cacgaacgcg
     1501 ggcaacgtca gcagcgcggc gacgtgccgg gcccagatcg gcggcgtgta gaggacgaac
     1561 ggggattggc gcgccagccc gtagccgtag atcagaacga caaacccgat cagcgacacc
     1621 agcgagtaga ggcccttcca acgctgctcg cccagatgca ggatctgctg cgcgcgccac
     1681 ccgccggcga cgatgcgcgt cgaatgcacg ccgagaaaca gcacgagtcc gaggaccagc
     1741 agtgacacgg cgggctcctt cacgaggcta ggaacgcgca gtatcgccca gcggcggatc
     1801 gaacagttcc gccgctcccg ccggatgtgc gtccggatcg cccgcgcgcg acagcgcgcc
     1861 gacccgcacg ccgagaaggc gaatgcgctt ggccaggtcg acgcgcttca ggcactgccc
     1921 ggcggcgcgg cggattgcct gcgcgtccgc gatcggcgcc ggcagcgtga tcgcgcgcgt
     1981 cacggtgcgg aagtcatcga agcgcagctt gatcgcgacg ttgcggccgg cgtagtgctt
     2041 gcggcgcagg tcgtcggaga cctcctcgca cagcgcggtg aagacccgcg acagggcggt
     2101 gcggtcgcgc accgcgtgca ggtcgcgttc gaacgtcgtc tcgcggctca tcgacaccgg
     2161 ctcgctgtgt gtgacgaccg ggcgctcgtc gcgtccgtgc gcggcatcgc agagccacgc
     2221 gccgtagctc gcaccgaagt gcgcgatcag ccagtcgggc gcggcggccg ccaactcgcc
     2281 gatcgtcgca atgccgagcc gcgccagttt cgccgctgac ttcggcccga cgccgttgat
     2341 ccgccgcacg ggcagcggcc agatacgcac ggcgagatcg tccatggtga cgacggtcag
     2401 cccgttcggc ttgtcgagct ccgaggccat cttggcgatc agcttgttgg gcgccagccc
     2461 gatcgagcag gtgagccccg ttgcgtcgcg caccgcctgc ttcagttcct gggccacctc
     2521 gcgcgcgcac caccatgcgt ccgcttccgt ggcgtcggca tcgtcgatcc gggcggcgac
     2581 ggcgt
//
LOCUS       DBNHBY010000302         8636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_356898, whole genome shotgun sequence.
ACCESSION   DBNHBY010000302 DBNHBY010000000
VERSION     DBNHBY010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_356898"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..634)
                     /gene="thrH"
                     /locus_tag="AC16HB_07395"
     CDS             complement(14..634)
                     /gene="thrH"
                     /locus_tag="AC16HB_07395"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HIR5606653.1"
                     /translation="MQVVCLDLEGVLVPEIWIEFSRRTGIPEFARTTRDEPDYDKLMR
                     FRLGLLRAHGLKLADIQKTIGAMSPLPGAREFLDDLRARYQVIILSDTFYEFADPLMR
                     QLGRPTLFCHRLHIDAEGFVADYRLRQPDQKRQAVHALKNLNFQVIAVGDSYNDTGML
                     GAADAGFFIHPPESIAAQFPQFPVNRSFAELMQSIDRAAARLVPTK"
     gene            complement(689..2113)
                     /gene="hemG"
                     /locus_tag="AC16HB_07400"
     CDS             complement(689..2113)
                     /gene="hemG"
                     /locus_tag="AC16HB_07400"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR5606654.1"
                     /translation="MFEGFAGNAHPSADVIVVGAGLSGLACAFRLARAGLQVEVLEAA
                     ARPGGVIGSRRRDGVLIESGPNSALDTSPLIGELLRDAGIDAERLGASPIADRRYILR
                     GGQLIAMPLSPPAFLATPLFSWRTKLGLLREPFIARAPADAEESVADFVRRRLGPEFL
                     DYAIEPFVAGIYAGDPELLSVKAAFPRLHTLEQTYGSLIRGMILGARERRRNAEKAKN
                     TAKSFSFRDGMQTLTDALVRGVGAERIRCSVHVDRVQREDDGRFTVEAATERERFART
                     ARALVLAVPAYEAARLVAPLAPATATALAEIVYPPVASVVSAYRRSDVAHALDGFGFL
                     APRKERPPVLGTLFSSSMFEGRAPADVVALTTFVGGRRNPALAREEPAQIAANVHASL
                     RNYVGAGAPPLWSEVTRWERAIPQYAFGHLARIASAESAEQACAGLYFCANYRGGVSV
                     GDCIKASHGLAERVAAQFGQRVAA"
     gene            complement(2467..3270)
                     /gene="ccmI"
                     /locus_tag="AC16HB_07405"
     CDS             complement(2467..3270)
                     /gene="ccmI"
                     /locus_tag="AC16HB_07405"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR5606655.1"
                     /translation="MTLFLALAVAMTAGAMAIVLLGSRARLGGASRAEANVAVLRQQL
                     HELERERGALACAEYEALRNEIERRLLLETPTHAAAVAARSRWPLVATTALLPVLAAA
                     VYVAVGAPQTMQDGDAPTLMQLESRVASVPRDARAWVLLARLRMDADQFESAALAYAK
                     AIAASSRVAADPQVWAEYADALGMSQGGSLAGPPREAIGHALALDPKHPKALELAGSA
                     AYEARDFRAAYAHWTALLAQLEADSPQRAPLAAAVAKAERLARFALPPS"
     gene            complement(3358..5064)
                     /locus_tag="AC16HB_07410"
     CDS             complement(3358..5064)
                     /locus_tag="AC16HB_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR5606656.1"
                     /translation="MLRISNSLLRVAVALAALVFLGMAASPSMAQQKKAAAKPAVAKF
                     DSGPCMLCHTPIKNFHDEGKHKGVGCNTCHQGLEQHLASQNQRPVTLTDPAVCGTCHK
                     PQFETMYRMNFDKTARFEKSQLTGPSPNPMWDKLMTPHGFTREHNLPRSHAFALYDQF
                     VVDRAFGGRFENKEDWRGLAKAGGNFKLQDIIRDADPANGDQKSFKPGTATAANPVCL
                     SCKTQDHILDWAYMGDPVPGAKWSRTSKVVEMARAENHSLNCFFCHDPHSAKPRVVRD
                     ALIQALTRPEKDTLWHNDPRGAKIDVKDMGLRGYTRKIAILSRYDTNLQCGQCHVEYN
                     CNPGTDPKTGQPITMADQRTNIFPFQKVDQIKKFYDDRNFRDFRHGITGALLWKAQHP
                     DTEIYYGSKHQKAGVECSQCHMPKIKDAKTGKTFTSHWQTSPKHYLKETCLTCHSEWK
                     AEQASYVIDSMKARYNGKLRKAEYWLTRMIDRFEEAKSLGVDEAVLNQVRGKHDEAHI
                     NWEFWTAANGAYFHNLNEATDSINKSMTISQEGIKLLDDAMTAKRKALTSAAAAPPAE
                     KK"
     gene            complement(5387..6646)
                     /gene="ccmI"
                     /locus_tag="AC16HB_07415"
     CDS             complement(5387..6646)
                     /gene="ccmI"
                     /locus_tag="AC16HB_07415"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR5606657.1"
                     /translation="MTQFIVIATLMVVVGLAWVLLPLLRRRSAADVDHAATNLRILKD
                     QLAELEAEKARGALSAVQYAETKAELERRVLDESRTEPVAPRSPASWYGRVSAAVIVV
                     AIPVAAALLYVKLGDPGAFDPLAQKTADDAHQLSTEQIEQMVAKLAERLKNEPDNAGG
                     WSMLARTYYFQRKFPEAAQAYKRLVELVPDDASVLADYADALAMANERRLAGEPMALI
                     KRALAIEPTQWKALAMAGTEAFDRKDYKGAVDYWERLRTSLPPESPIAQQIGNSIAEA
                     RQLGGLKPAEGEAKVAAAAPAAKSAAPAGAAQVSGVVTLSPKLTANAKPDDAVFIFVR
                     AAQGPRMPLAIIRLQVKDLPAKFSLDDSMAMSPDFKLSKFSEVVVGARVAKGGVANAQ
                     RGDLEGLSQPVKLGSRDINVQIDTVVQ"
     gene            complement(6643..7101)
                     /locus_tag="AC16HB_07420"
     CDS             complement(6643..7101)
                     /locus_tag="AC16HB_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR5606658.1"
                     /translation="MRALIAFVFLLVAAAAHAQTAQPTENDPVAAKREYELALKLRCL
                     VCQNQSIAESNASLAVDLRNQIREQISSGKSDREIITFMTDRYGDFVLYQPPFKATTA
                     LLWGGPVLLLAIGALVLVRVVRARRATAAPLSDAERARAEALLAGKESGQ"
     gene            complement(7098..7634)
                     /locus_tag="AC16HB_07425"
     CDS             complement(7098..7634)
                     /locus_tag="AC16HB_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR5606659.1"
                     /translation="MKKAALYLIPLAAFVVLAVFLLRGLWLNPREVPSPLIGKPAPMF
                     ERPELLQPQAQFDSKKMLGQVWVFNVWASWCTPCRQEHPLWIELATRKLVPIVGLNYK
                     DEPGAAKRWLTDLGDPYTQIVVDGDGRLGIDYGVYGVPETFIIDKQGVIRHKHIGPVT
                     PEAMEKKIVPLVRELQKS"
     gene            complement(7642..>8636)
                     /locus_tag="AC16HB_07430"
     CDS             complement(7642..>8636)
                     /locus_tag="AC16HB_07430"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR5606660.1"
                     /translation="PRVGIGGAFSAVSRESMLLANNVLLTVACGSVLLGTLYPLFLDA
                     LNLGKISVGPPYFNSVFVPIMAPLVFLMGVGPLARWRQASVPDLWTQLRWALVVSLVL
                     GLVLPFVGGSWSPMLAFGYWLAAWIVMSAIEQIRTKIRNSRGGLFARLASQPRGWWGM
                     TLAHLGIAVFIVGVTSVKGYEVERDVKMNVGDTISVRDYTFRFDGVTERAGPNYRAAR
                     GAVTVTKGGKQDMVLHPEKRIYLAQQGMPMTEAAIDTGITGDVYVSLGEAVDTDGQSR
                     GAWTVRVYYKPFVTWVWGGCVLMALGGFVAMSDRRYRSLARRDELTIGLKPASA"
BASE COUNT         1301 a         2983 c         2924 g         1428 t
ORIGIN      
        1 cgcgccgcgt agctcactta gtcggaacga gccgcgccgc ggcgcggtcg atcgactgca
       61 tcagttccgc gaagctgcgg ttgaccggaa actgcggaaa ctgcgcggcg atcgactccg
      121 gcgggtggat gaagaagccc gcatcggcgg caccgagcat gccggtgtcg ttgtacgaat
      181 cgcccacggc gatgacctgg aagttcaagt tcttcagcgc gtggactgcc tggcgcttct
      241 ggtccggctg acgcagccgg taatcggcga cgaaaccctc cgcatcgata tgcagccgat
      301 ggcaaaaaag cgtcggccgg ccgagctggc gcatcagcgg atcggcgaac tcgtaaaacg
      361 tgtcggacag gatgatcacc tggtagcgcg cgcgcaaatc gtccaggaac tcgcgcgcgc
      421 ccggcagcgg actcatcgcg ccgatcgtct tctggatgtc ggcgagcttc agcccgtgtg
      481 cgcgcagcag gccgaggcgg aagcgcatca gcttgtcgta gtcgggctca tcgcgcgtgg
      541 tgcgcgcgaa ctccgggatg ccggtgcggc gggagaactc gatccagatc tcgggcacga
      601 ggacgccttc gagatcgagg cagacgactt gcattcggaa ctccgtgatc aggccgcgcg
      661 catcatacga tccgcgcgcg cgagcgcgtc acgccgcgac gcgctgtccg aactgcgcgg
      721 ccacgcgttc ggccagtccg tgcgacgcct tgatgcagtc gccgacggat acgccgccgc
      781 ggtagttggc acagaagtac aggcccgcgc acgcctgctc cgcgctctcg gcgctggcaa
      841 tgcgcgcgag gtgcccgaac gcgtactgcg ggatcgcgcg ctcccagcgc gtcacctcgc
      901 tccacagcgg cggcgcgccc gcgccgacat agttacgcag cgatgcgtgc acattggccg
      961 cgatctgcgc cggctcctcg cgcgcgagcg cggggttgcg ccggccgccg acaaaggtcg
     1021 tcagggcgac gacgtcagcc ggcgcgcggc cctcgaacat cgagctggag aacagcgtgc
     1081 cgagcaccgg cggccgctcc ttcctcggcg cgagaaagcc gaagccgtcg agcgcatgcg
     1141 cgacatcgct ccggcggtat gcgctgacca cgctcgccac cggcgggtag acgatctccg
     1201 cgagcgcggt cgccgtcgcg ggtgcgagcg gcgccaccag ccgcgccgct tcgtaggcgg
     1261 gtaccgcgag caccagcgcg cgcgcggtac gcgcgaagcg ctcgcgctcg gtcgccgctt
     1321 cgaccgtgaa ccggccgtca tcctcgcgct gcacgcggtc gacgtgcacg ctgcagcgga
     1381 tgcgctcggc cccgacgccg cgcaccagcg cgtcggtcag cgtctgcatg ccatcgcgga
     1441 acgaaaagct cttggcggtg ttcttcgctt tttccgcgtt gcgcctgcgc tcgcgcgcgc
     1501 cgaggatcat gccgcggatc aggctgccgt aggtctgctc gagcgtgtgc agacgcggaa
     1561 acgccgcctt gaccgacagc agctcgggat cgccggcgta gatgccggcg acgaagggct
     1621 cgatcgcgta gtcgaggaat tcggggccca ggcggcgccg cacgaagtcg gccacgctct
     1681 cctcggcatc ggcgggggca cgggcaatga acggctcgcg caggagcccg agcttggtgc
     1741 gccacgaaaa gagcggcgtg gcgagaaacg ccggcggcga cagcggcatc gcgatcagtt
     1801 gcccgccgcg caggatgtaa cggcggtccg cgatcgggct cgcgccgagc cgctcggcgt
     1861 cgatcccggc gtcgcgcagc agttcgccga taagcggcga ggtgtcgagc gcactgttgg
     1921 gccccgactc gatcagcacg ccgtcgcggc ggcgcgagcc gatcacgccg ccgggccgcg
     1981 cggccgcttc gagcacctcg acctgcagcc cggcgcgcgc gagtcggaac gcgcacgcga
     2041 gccccgatag cccggccccg accacgatca catccgcgct cggatgcgca ttgcccgcga
     2101 aaccctcgaa catcgacgct ccctcgcctg cgacgaatca aggctaggcg cttgccgcgc
     2161 cgtgccgcgt gatgccggtc aagccgcgca ctatcccgca gccgaccatg aagagcgatt
     2221 gtccacccgc cgtttcgcgc ttgcctgcga ggcgttgtag accgtgcata cacacttggt
     2281 ccccgcgagt gacagctcgg cgccgtgcgc cgcgcagcga ctggccggtg ccgcctacgg
     2341 atgccccttc gcgaccgaca ggccgcgtgc ggctgcgaag ctgaacggcg gcgtcgcgag
     2401 cgcgcgcagg tgcgcggcgg tcgtcgtcac gacgatgcca ggcacgttta tttcggaatc
     2461 tacgcttcag gacggcggca gtgcgaaacg tgcaagtcgc tcggccttcg cgaccgcggc
     2521 cgcgagcggc gcgcgctgcg gcgaatccgc ctctagctgc gcgagcagtg cggtccagtg
     2581 cgcgtacgcg gcgcggaaat cccgcgcctc gtaagcggca ctgcccgcca gttcgagcgc
     2641 cttcggatgt ttcgggtcga gcgcgagcgc atggccgatc gcctcgcgcg gcgggcccgc
     2701 gagcgatccg ccctggctca tgccgagcgc gtccgcgtac tcggcccaga cctgcggatc
     2761 ggccgcgacc ctggacgaag ctgcgattgc cttcgcatac gccagcgcgg cggactcgaa
     2821 ctgatccgcg tccatgcgca gccgtgccag cagcacccac gcgcgcgcat cgcgcggcac
     2881 cgacgcgacg cgtgattcga gctgcatgag cgtcggcgcg tcgccatcct gcatggtttg
     2941 cggcgcgccc accgcgacgt acaccgcggc ggcgagtacc ggcagcagcg ccgtcgtcgc
     3001 gaccagtggc cagcgcgacc gcgccgcgac cgccgccgcg tgcgtgggtg tctcgagcag
     3061 cagccggcgt tcgatttcat tgcgcaacgc ctcgtactcg gcgcacgcga gtgcaccgcg
     3121 ctcgcgctcg agttcgtgca gctgctggcg caacaccgca acgttggctt cggcccgcga
     3181 cgctccgccc aggcgcgcgc gcgagccgag cagcacgatg gccatcgcgc cggccgtcat
     3241 cgccaccgcc aaggcaagga acaacgtcat tgacgcacct cgcgcgcggc ggcccgggcc
     3301 gtcaggcgcc cgaccgaacg cgcgcaggca ttccggcgca ttggatggcc cggaagctca
     3361 cttcttctct gccggaggcg ctgccgcggc cgaagtgagt gccttgcgct tcgcggtcat
     3421 cgcgtcgtcg agcagcttga tgccttcctg cgagatcgtc atcgacttgt tgatcgagtc
     3481 ggtcgcctcg ttcaggttgt ggaagtacgc gccgttggcg gcggtccaga attcccagtt
     3541 gatgtgcgct tcgtcatgct tgccgcgcac ctggttgagc accgcttcgt cgacgccgag
     3601 gctcttcgct tcctcgaacc ggtcgatcat gcgcgtcagc cagtactcgg ccttgcgcag
     3661 cttgccgttg tagcgcgcct tcatgctgtc gatcacgtag ctcgcctgct cggccttcca
     3721 ctcgctgtgg cacgtgaggc aggtttcctt caggtaatgc ttgggacttg tctgccagtg
     3781 cgacgtgaag gtcttgccgg tcttcgcgtc cttgatcttc ggcatgtggc actgcgagca
     3841 ttcgacgccg gccttctggt gcttgctgcc gtagtagatc tcggtgtcgg ggtgctgcgc
     3901 cttccacagc agcgcgcccg tgatgccgtg gcggaagtcg cggaagttgc ggtcgtcgta
     3961 gaacttcttg atctgatcga ctttctggaa cgggaagatg ttcgtgcgct gatccgccat
     4021 cgtgatcggc tgcccggtct tcggatcggt acccggattg cagttgtatt cgacgtggca
     4081 ctggccgcac tgaagattcg tgtcatagcg cgacaggatc gcgatcttgc gcgtgtagcc
     4141 gcgcaggccc atgtccttga cgtcgatctt ggcgccgcgc ggatcgttgt gccacagcgt
     4201 gtccttctcg ggacgcgtca gcgcctggat cagcgcgtcg cgcacgacgc gcggcttcgc
     4261 gctgtgcggg tcgtggcaga agaagcagtt cagcgagtgg ttctcggcgc gcgccatctc
     4321 gaccaccttc gacgtgcggc tccacttggc acccggcacc gggtcgccca tgtaagccca
     4381 gtcgaggatg tggtcctgcg tcttgcacga caggcacacc ggattggccg cggtcgcggt
     4441 gccgggcttg aacgacttct ggtcgccgtt ggcgggatcg gcgtcgcgga tgatgtcctg
     4501 cagcttgaag ttgccgccgg ccttggccag cccgcgccag tcctccttgt tctcgaagcg
     4561 gccgccgaac gcgcgatcga cgacgaactg gtcgtacagc gcgaacgcgt gcgagcgcgg
     4621 caggttgtgc tcgcgcgtga agccgtgtgg tgtcatcagc ttgtcccaca tcgggttggg
     4681 cgacgggccc gtgagctgcg atttctcgaa acgcgcagtc ttgtcgaagt tcatccggta
     4741 catcgtctcg aactgcggct tgtggcaggt gccgcacacg gcggggtcgg tcagcgtgac
     4801 cggccgctgg ttctggctcg cgaggtgctg ctcgagtccc tggtggcagg tgttgcagcc
     4861 cacgcccttg tgcttgccct cgtcgtggaa gttcttgatc ggcgtgtggc acagcatgca
     4921 ggggcccgaa tcgaatttcg ccaccgcggg cttggctgcg gccttcttct gctgcgccat
     4981 ggacggcgac gctgccatgc cgaggaacac caaagccgcg agcgcgacgg caacgcgcag
     5041 cagcgaattg ctgatacgga gcatcccttc ctcccgttcg attgagcttt gccgcgccgt
     5101 gtatggcgcg caccgattgg aagtctccga gtcgccatcg ggggctcgtt gacgactgtc
     5161 aacgcgcgcg accggccgcg ttatctcttt gggggtacag caacgggctc cgttacagcc
     5221 gcgccgcgcg tcgcgctgcg cgagctacgg ctcggccggg ttgtcggcga gatgcggctt
     5281 cgcttcgacg tggcagacag cgccgttgca gaacttgcgc cgggccggtg cggaacgtgc
     5341 ggtgcggctg cgtgcggcgg aatcggccgc cggatgatga tgcgcgctac tgcacgaccg
     5401 tatcgatctg cacgttgatg tcgcggctgc cgagcttgac gggctgcgac aagccctcga
     5461 ggtcgccgcg ctgcgcgttc gccacgccgc ccttggcgac gcgcgcgccg accacgactt
     5521 cgctgaactt cgacaacttg aagtcgggcg acatcgccat cgaatcgtcg agcgagaatt
     5581 tcgccggtag atccttgacc tggagcctga tgatcgccag cggcatgcgc ggcccctgcg
     5641 ccgcgcgcac gaagatgaat accgcatcgt ccggcttagc attggcggtg agcttcgggc
     5701 tcagcgtgac cacgccgctg acctgcgcag cgccggccgg cgccgcgctc ttggccgcgg
     5761 gcgccgccgc ggcgaccttc gcttcgcctt cggccggttt caatccgccg agctgacgcg
     5821 cctcggcgat cgagttgccg atctgttgcg cgatcggcga ctccggcggc agcgaggtgc
     5881 gcaggcgctc ccagtagtcg acggcaccct tgtagtcctt gcggtcgaac gcttccgtgc
     5941 cggccatggc cagcgccttc cactgcgtcg gctcgatcgc cagtgcgcgc ttgatcagcg
     6001 ccatgggttc gcccgcgagc cggcgttcat tggccatcgc cagcgcgtcc gcgtaatcgg
     6061 ccagcacgga cgcatcgtcg ggtaccagtt cgacgagccg cttgtatgcc tgcgctgcct
     6121 ccgggaactt gcgctggaag tagtacgtgc gcgccagcat cgaccagccg ccggcgttgt
     6181 ccggttcgtt cttcaggcgc tcggcgagct tggcgaccat ctgctcgatc tgctcggtgc
     6241 tcagctgatg cgcgtcgtcg gcggtctttt gcgcgagcgg atcgaaggcg cccgggtcgc
     6301 cgagcttcac gtacagcagc gcggccgcga ccgggatcgc cactacgatc accgctgcgc
     6361 tcacccgccc gtaccagctt gcgggcgagc gcggcgcgac cggctcggtc cgcgattcgt
     6421 ccagcacgcg ccgctcgagc tctgctttgg tctcggcgta ctgcacagcg gacagcgcgc
     6481 cgcgtgcctt ttcggcctcg agctcggcga gctggtcctt caggatgcgc agattggtcg
     6541 ccgcgtggtc gacgtcggct gcggagcgtc gacgcagcag cggcagcagc acccacgcga
     6601 ggccgacaac gaccatcagc gtcgcgatga cgatgaattg cgtcactgtc cgctctcctt
     6661 gcccgcgagc agtgcctcgg cacgggcgcg ctcggcgtcc gacagcggcg cggccgtcgc
     6721 gcggcgcgcg cgcacgacgc gcaccagtac cagcgcgccg atcgccagca gcagcaccgg
     6781 cccgccccac agcaacgccg tggtggcctt gaacggcggc tggtacagca cgaagtcgcc
     6841 gtagcggtcg gtcatgaagg tgatgatctc gcgatcgctc ttgccgctgc tgatctgctc
     6901 gcggatctgg ttgcgcaggt cgaccgccag gctggcgttc gactcggcga tcgactggtt
     6961 ctggcacacc aggcagcgca gcttcaaggc gagctcgtat tcgcgcttgg cggcgaccgg
     7021 gtcgttctcg gtcggctgcg cggtctgcgc atgcgctgcg gcggccacga gcaggaagac
     7081 aaaggcgatc agggctctca tgatttctgc agttcgcgca ccagcggcac gatcttcttc
     7141 tccatcgcct cgggcgtgac cggtccgatg tgcttgtggc ggatcacgcc ctgcttgtcg
     7201 atgatgaagg tttcgggcac accgtagacg ccgtagtcga tgccgagccg gccgtcgccg
     7261 tcgacgacga tctgcgtgta cggatcgccg aggtcggtga gccagcgctt ggccgcgccg
     7321 ggctcgtcct tgtagttcag gccgacgatc ggaaccagct tgcgcgtcgc cagctcgatc
     7381 cacagcggat gctcctggcg gcacggcgtg caccacgacg cccacacgtt gaacacccac
     7441 acctgcccga gcatcttctt gctgtcgaac tgcgcctgcg gctgcagcag ctcgggccgc
     7501 tcgaacatcg gcgccggctt gccgatcagc ggcgacggca cctcgcgcgg gttcagccac
     7561 aggccgcgca gcaggaacac cgccagcacg acgaaggcgg cgagcggaat caggtacagc
     7621 gcggctttct tcatcacccg ctcacgccga ggcaggcttc agcccgatcg tcagttcgtc
     7681 gcgccgcgcg agcgaccggt agcggcggtc ggacatcgcg acgaagccgc cgagcgccat
     7741 cagcacgcag ccgccccaga cccacgtcac gaacggcttg tagtagacgc gcacggtcca
     7801 ggcgccgcgc gactgcccgt ccgtgtcgac cgcctcgccg agggaaacgt atacgtcgcc
     7861 ggtgatgccg gtgtcgatgg cggcctcggt catcggcatg ccctgctgcg cgaggtagat
     7921 gcgcttctcg ggatgcagca ccatgtcctg cttgccgccc ttggtcaccg tcacggcacc
     7981 ccgcgccgcg cggtagttgg gtcccgcgcg ctcggtcacg ccgtcgaagc ggaacgtgta
     8041 gtcgcgcacg ctgatcgtgt cgccgacatt catcttcacg tcgcgctcga cctcgtagcc
     8101 cttgaccgac gtgacgccga cgatgaacac ggcgatcccc aggtgcgcga gcgtcatgcc
     8161 ccaccagccg cgcggctggc tggcgagccg cgcgaacagt ccgcccctcg aattgcggat
     8221 cttggtgcgg atctgctcga tcgcagacat cacgatccac gcggcgagcc agtagccgaa
     8281 cgcgagcatc ggcgaccagg acccgcccac gaatggcagc acgagcccga gcaccagcga
     8341 aacgacgagc gcccagcgca gctgcgtcca caggtccgga accgacgcct gccgccagcg
     8401 cgcgagcgga ccgacgccca tcaggaacac cagcggcgcc atgatcggca cgaacaccga
     8461 gttgaagtac ggcgggccga cggagatctt gccgaggttc agcgcgtcga ggaacagcgg
     8521 atacagcgtg ccgagcagca ccgacccaca ggcgaccgtc agcagcacat tgttggcgag
     8581 cagcatcgat tcgcgcgaca ccgcggaaaa cgcgccgccg atgcccacgc gcggcg
//
LOCUS       DBNHBY010000303         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1236509, whole genome shotgun sequence.
ACCESSION   DBNHBY010000303 DBNHBY010000000
VERSION     DBNHBY010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1236509"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC16HB_07435"
     CDS             complement(<1..329)
                     /locus_tag="AC16HB_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR5606661.1"
                     /translation="MKDSIRRRLEKTVDRFEEVGRLLADPDVIARQGQFRELSMEYSR
                     LEPVAARFAAFRSLEAELKSNEEMAAGADAEMRELAAEEARDLQERLAAEDAELTKLL
                     IPKDPRD"
     gene            complement(353..>1626)
                     /gene="hemA"
                     /locus_tag="AC16HB_07440"
     CDS             complement(353..>1626)
                     /gene="hemA"
                     /locus_tag="AC16HB_07440"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR5606662.1"
                     /translation="TGIRMSLVVVGINHRTAPVEVRERVVFEPARIPDALQQLAALPE
                     VQESVIVSTCNRTELYCVTNGTQADLGGWLQRYHGLTNVALHHSLYHHDENQAVTHAF
                     EVASGLDSMVIGEPQILGQLKDAYRLAQETGTTGPVLNRLFQSAFSVAKRVRTETKIG
                     ANAVSVASAAVSMARTVFASFENRTALMVGAGETIALAARHLYADGLRRMIIANRSVE
                     RARELAGEFHGYAIGLDEIPNHLKEADIVVASTAAPHSIITRHMTEQALRARKRKPMF
                     MVDIAVPRDIDADVAELEDVYLFTVDDLQSVVNENLEGRRQAAREAGELIRAEVDKFS
                     HTLRTRDAAPLIRRLREDADRTRRHTLEQAQQMLAHGKSHEEVLNFLANTLTNRLIHA
                     PSQRLRDAAETGDGEVLETIAQIYKLDVTHD"
BASE COUNT          211 a          528 c          601 g          286 t
ORIGIN      
        1 tcgtcgcgcg ggtccttggg gatcagcagc ttcgtgagtt cggcgtcctc ggccgcgagg
       61 cgctcctgca ggtcgcgcgc ttcctccgcg gcgagttcgc gcatctcggc gtcggcgccc
      121 gcggccattt cctcgttgga tttcagctcg gcttcgaggc tgcggaaggc ggcgaagcgc
      181 gcggccaccg gctcgagccg cgagtactcc atcgaaagct cgcggaactg gccctggcgc
      241 gcgatgacgt cggggtcggc gagcaggcgg ccgacttcct cgaagcggtc gaccgtcttc
      301 tccagtcggc ggcggatgga gtccttcatg cgtgcgcgac cggtggcagc cgtcagtcgt
      361 gcgtcacgtc gagcttgtag atctgcgcga tcgtctcgag cacttcaccg tcgccggttt
      421 cggccgcgtc gcgcagccgc tggcttgggg cgtggatcag ccggttggtg agcgtgttgg
      481 cgaggaagtt gagcacctcc tcgtggctct tgccgtgcgc cagcatctgc tgcgcctgct
      541 ccagcgtgtg gcggcgcgtg cggtcggcat cttcgcgcag gcggcggatc agcggcgccg
      601 cgtcgcgggt gcgcagcgtg tgcgagaact tgtcgacctc ggcgcggatc agctcgccag
      661 cctcgcgcgc ggcctggcgc cggccctcga ggttctcgtt gaccaccgac tgcaggtcgt
      721 cgacggtgaa caggtagacg tcctcgagct cggcgacgtc ggcgtcgatg tcgcgcggca
      781 cggcgatgtc caccatgaac atcggcttgc gcttgcgcgc gcgcagcgcc tgctcggtca
      841 tgtgccgcgt gatgatcgaa tgtggcgcag cggtggaggc cacgacgatg tcggcctcct
      901 tgaggtggtt cgggatctcg tcgaggccga tcgcgtagcc gtggaactcg ccggcgagct
      961 cgcgcgcccg ctcgacgctg cggttggcga tgatcatgcg ccgcaggcca tcggcgtaca
     1021 ggtggcgcgc ggcgagcgcg atggtctcgc ccgcgccgac catcagcgcc gtgcggttct
     1081 cgaagctggc gaagaccgta cgggccatgg acaccgcggc cgaggccacc gatacagcgt
     1141 tggcgccgat cttggtctcg gtgcgcacgc gcttggccac ggagaacgcc gactggaaca
     1201 gccggttcag cacggggccg gtcgtgccgg tttcctgcgc gaggcggtag gcgtccttga
     1261 gctgaccgag gatctgcggc tcgccgatga ccatcgaatc gagcccggag gcgacctcga
     1321 aggcgtgcgt gacggcctgg ttctcgtcgt ggtggtacag gctgtggtgc agcgccacgt
     1381 tcgtgaggcc gtggtagcgc tgcagccagc cgcccaggtc cgcctgcgtg ccgttggtga
     1441 cgcagtagag ctccgtccgg ttgcacgtcg agacgatgac cgactcctgc acctccggga
     1501 gcgcagcgag ctgctgcagg gcatcgggga tccgcgcggg ctcgaacacc acgcgttcgc
     1561 gcacctcgac gggggcggta cggtggttga tgccgacgac gacgagagac atccggattc
     1621 cggtcg
//
LOCUS       DBNHBY010000304         2350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_763046, whole genome shotgun sequence.
ACCESSION   DBNHBY010000304 DBNHBY010000000
VERSION     DBNHBY010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_763046"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1483
                     /locus_tag="AC16HB_07445"
     CDS             14..1483
                     /locus_tag="AC16HB_07445"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HIR5606663.1"
                     /translation="MLTPAFTPPQRFDDPHAALAQARAIYSQSTDFLRDALDRFVAGE
                     DLPRRVRACYPFVRVRIDTVARADSRLSYGFVAGPGTFETTLTRPDLFERYYLEQFEL
                     LRANHGVTLEVGTSRQPIAVHFALTDDDYFEGTLSAERRLRMRDLFDLPDLGSMDDGI
                     ANGTYEPRGGEPQPLALFTAPRVDYSLHRLRHYTGSSPEHVQNFVLFTNYQFYVDEFV
                     RLGRDLVTGGDPAAHDYAAFVEPGNVITRRGQVTTGTPLARLPQMPAYHLVRDDGAGI
                     TMVNIGIGPANAKTITDHIAVLRPHAWIMLGHCAGLRTTQQLGDYVLAHGYVREDHVL
                     DEDLPTWVPIPALAEIQVALERAVADVTRLSGYELKRIMRTGTVASTDNRDWELLPQR
                     TPERRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLPGMANQFYR
                     ERVDQHLRIGMRAIELLRGQRPDQLHSRKLRSFSEVAFQ"
     gene            complement(1488..2258)
                     /locus_tag="AC16HB_07450"
     CDS             complement(1488..2258)
                     /locus_tag="AC16HB_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HIR5606664.1"
                     /translation="MGGVVLDRGLEAALRHDRRVVAASLALVVALAWLYLWHDAAMMR
                     AMPDMPRASVPGQFVMAFVMWTVMMVGMMLPSAAPAILLYGTLVRKHSEQGRVLPSAW
                     VFSGGYIAVWTAFSAAAALLQMLLDRAALLSPALGSADSALSAAILVAAGIYQWLPLK
                     DACLRRCRDPLTFFMTRWRGGRAGALAMGAEHGVYCLGCCWALMGVLFVAGVMNLLWV
                     ALIAAFVLVEKLLPASRYTSRIAGAALVIAGAALFAAR"
BASE COUNT          389 a          860 c          779 g          322 t
ORIGIN      
        1 tccggctgcg cccgtgctga cacccgcatt cactccgccg cagcgcttcg acgatccgca
       61 tgccgcgctg gcgcaggcgc gcgcgatcta ttcgcagtcg acggacttcc tgcgcgatgc
      121 gctcgaccgt ttcgtggcgg gcgaggatct gccgcggcgc gtgcgcgcgt gctatccgtt
      181 cgtgcgcgtg cgcatcgaca ccgtcgcgcg cgccgattcg cgcctgagct acggcttcgt
      241 cgccggcccg ggcaccttcg aaaccacgct gacgcgtccg gacctgttcg aacgctacta
      301 cctcgagcag ttcgagctgc tgcgcgccaa tcacggcgtc acgctcgaag tcggcacgag
      361 ccggcagccg atcgcggtgc acttcgcatt gaccgacgac gactacttcg agggcacgct
      421 ttcggccgag cggcggctgc ggatgcgcga cctgttcgac ctgccggatc tcggcagcat
      481 ggacgacggc atcgccaacg gcacctacga gccccgcggc ggcgagccgc agccgctggc
      541 gctgttcacc gcgccccgcg tggactactc gctgcaccgg ctgcgccact acacgggttc
      601 gtcgcccgag cacgtgcaga acttcgtgct gttcaccaac taccagttct atgtcgacga
      661 attcgtccgc ctcggccgcg acctcgtgac cggcggcgat ccggccgcgc acgattacgc
      721 ggcgttcgtc gagccgggta acgtgatcac gcgccgcggg caggtcacga ctggaacgcc
      781 gctcgcgcgg ctgccgcaga tgcctgcgta tcacctggtg cgcgacgacg gtgccggcat
      841 cacgatggtc aacatcggca tcggccccgc caacgccaag acgatcaccg atcacatcgc
      901 cgtgctgcgg ccgcacgcct ggatcatgct cggccactgc gccgggttgc gcaccacgca
      961 gcagctcggc gactatgtgc tcgcgcacgg ctacgtgcgc gaggaccacg tgctcgacga
     1021 agacctgccg acctgggtgc cgatcccggc gctggcggaa atccaggtgg cgctcgagcg
     1081 ggcggtggcg gacgtgacgc ggctctcggg ctacgaactc aagcgcatca tgcgcaccgg
     1141 caccgtggcg agcaccgaca accgcgactg ggagctgctg ccacagcgca cgccggaacg
     1201 ccgcttcagc cagagccgcg ccatcgcgct cgacatggaa agcgcgacga tcgccgccaa
     1261 cggcttccgc tttcgcgtgc cgtacgggac gctcctgtgc gtgagcgaca aaccgctgca
     1321 cggcgagatc aagctgccgg gcatggcgaa ccagttctat cgcgagcgag tcgaccagca
     1381 cctgcgcatc ggcatgcgcg cgatcgagct gctgcgcggc cagcggccgg accagctgca
     1441 cagccgcaag ctgcgcagct tttccgaggt cgcgtttcag tagtgcttca gcgcgcagca
     1501 aacagcgcgg cgccggcgat caccagcgcg gcgccggcga tgcgcgatgt gtagcgcgac
     1561 gccggcagca gcttttcgac caggacaaag gcggcgatca gcgccaccca caacaggttc
     1621 atcacgccgg cgacgaacag cacgcccatc agcgcccagc agcagcccag gcagtagacg
     1681 ccgtgctcgg cgcccatcgc cagcgccccg gcgcggccgc cgcgccagcg cgtcatgaag
     1741 aaggtcagcg ggtcgcggca cctgcgcagg caggcatcct tcagcggcag ccactggtag
     1801 atgccggccg cgacgaggat cgcggcgctc aacgcgctgt cggcggaacc gagcgccggc
     1861 gacagcagcg cggcgcgatc cagcagcatc tgcagcagtg cggcggcggc gctgaacgcc
     1921 gtccagaccg cgatgtagcc gccgctgaag acccacgccg atggcagcac gcggccctgt
     1981 tcgctgtgct tgcgcaccag cgtgccgtac agcaggatcg ccggcgcggc gctgggcagc
     2041 atcatgccga ccatcatcac ggtccacatc acgaacgcca tgacgaactg gcccgggaca
     2101 gaagcgcgcg gcatgtccgg catcgcgcgc atcatcgccg cgtcgtgcca aagatagagc
     2161 catgcgagcg cgaccaccag cgcgaggctt gccgccacca cgcggcggtc gtgccgcagc
     2221 gcggcctcga ggccgcggtc gaggactacg ccgcccacgc gtagcgcgcg aagtggccgt
     2281 tgcgcttgcc gctcgagtcg tcccacttga tcccgaacgc gtcgaagcgc gagcgcgtcg
     2341 cgagcgcgac
//
LOCUS       DBNHBY010000305         1769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1642283, whole genome shotgun sequence.
ACCESSION   DBNHBY010000305 DBNHBY010000000
VERSION     DBNHBY010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1642283"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..>1769)
                     /gene="rpoB"
                     /locus_tag="AC16HB_07455"
     CDS             complement(37..>1769)
                     /gene="rpoB"
                     /locus_tag="AC16HB_07455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5606665.1"
                     /translation="GDVLADGPSTDLGELALGQNLLVAFMPWNGYNFEDSILISERVV
                     EEDRFTTIHIEELTCVARDTKLGPEEITADIPNVGENALAKLDEAGIAFIGAEVKAGD
                     ILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPPGMDGTVIDVRVFTRDGV
                     EKDSRALAIEKSEIEKVRKDLADQQRILEEDLFSRVHSMLVGKVASAGPRKLKAGSKV
                     TADYLADLPREHWLEVRLEDEEANASLELTAERMRSQRKEFERRLEEKRQKITQGDDL
                     APGVLKMVKVYLAVKRRVQPGDKMAGRHGNKGVISTIVPVEDMPFMADGTPVDIVLNP
                     LGVPSRMNIGQILETHLGWAAKGLGIKIGNMLDAGRSAADVRGLLEQVYNHGDGKRED
                     LRSLNDDDIVALGNNLRAGVPMATPVFDGAAEAEIKYMLKLADLPDSGQTTLYDGRTG
                     ERFERDVTVGYMYMLKLNHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGASYTLQEMLTVKSDDVTGRTKMYKNIVDGNHQMDAGMPESFNVLVKEIRSLG
                     INIELHQEKR"
BASE COUNT          226 a          605 c          595 g          343 t
ORIGIN      
        1 gggtcacctg cgcaggggag acggccggcc ggccgctcac cgcttttcct ggtggagttc
       61 gatgttgatg ccgagcgagc ggatttcctt caccagcacg ttgaaggatt ccggcatgcc
      121 tgcgtccatc tggtgattgc cgtccacgat gttcttgtac atcttggtgc ggccggtcac
      181 gtcgtcggac ttgacggtca gcatctcctg cagcgtgtac gaggcaccgt aggcctcgag
      241 cgcccagact tccatttcgc cgaagcgctg gccgccgaac tgcgccttgc cgcccagcgg
      301 ctgctgcgtg acgaggctgt acgggccggt cgaacgcgcg tgcatcttgt cgtcgaccag
      361 gtggttgagc ttcagcatgt acatgtagcc gaccgtgacg tcgcgctcga agcgctcgcc
      421 cgtgcggccg tcgtacagcg tggtctggcc gctgtcgggc aggtcggcca gcttcagcat
      481 gtacttgatc tcggcctcgg ccgcgccgtc gaacaccggc gtcgccatcg gcacgcccgc
      541 acgcaggttg ttgcccagcg cgacgatgtc gtcgtcgttc agcgagcgca ggtcctcgcg
      601 cttgccgtcg ccgtggttgt agacctgctc gagcagcccg cgcacgtcgg ccgcgctgcg
      661 gccggcgtcg agcatgttgc cgatcttgat gccgaggccc ttggcggccc agccgaggtg
      721 ggtctcgagg atctggccga tgttcatgcg cgacggcacg cccagcgggt tcagcacgat
      781 gtcgaccggc gtgccgtccg ccatgaacgg catgtcctcg accggcacga tcgtggagat
      841 cacgcccttg ttgccgtggc ggccggccat cttgtcgccc ggctgcacgc ggcgcttcac
      901 cgcgaggtag accttgacca tcttgagcac gcccggtgcg aggtcgtcgc cctgcgtgat
      961 cttctggcgc ttctcctcga ggcggcgctc gaactccttg cgctgcgagc gcatgcgctc
     1021 ggcggtgagc tcgaggctgg cgttggcctc ctcgtcctcc agccgcactt cgagccagtg
     1081 ctcgcgcggc aggtcggcca ggtagtcggc ggtgaccttg ctgcccgcct tgagcttgcg
     1141 cgggcccgcg gaggcgacct tgcccaccag catggagtgc acgcgcgaga agaggtcctc
     1201 ctcgaggatg cgctgctggt cggcgaggtc cttgcggacc ttctcgatct cggacttctc
     1261 gatggccagc gcgcggctgt ccttctcgac gccgtcgcgc gtgaacacgc gcacgtcgat
     1321 cacggtgccg tccatgccgg gcggcacgcg cagcgaggtg tccttcacgt cggacgcctt
     1381 ctcgccgaag atcgcgcgca gcagcttctc ttccggcgtg agctgcgtct cgcccttcgg
     1441 cgtgaccttg cccaccagga tgtcgcccgc cttgacctcg gcgccgatga aggcgatgcc
     1501 ggcctcgtcg agcttggcca gcgcgttctc gccgacgttc gggatgtccg ccgtgatctc
     1561 ctcggggccg agcttggtgt cgcgcgcgac gcaggtcagc tcctcgatgt ggatcgtggt
     1621 gaagcggtct tcctcgacca cgcgctccga gatcaggatc gagtcctcga agttgtagcc
     1681 gttccacggc atgaacgcga ccagcaggtt ctggccgagc gcgagctcgc cgaggtccgt
     1741 cgacgggccg tcggccagca cgtcgccgc
//
LOCUS       DBNHBY010000306         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1236736, whole genome shotgun sequence.
ACCESSION   DBNHBY010000306 DBNHBY010000000
VERSION     DBNHBY010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1236736"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1393
                     /locus_tag="AC16HB_07460"
     CDS             260..1393
                     /locus_tag="AC16HB_07460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family alpha/beta hydrolase"
                     /protein_id="HIR5606666.1"
                     /translation="MTDLVEQMHRTVGDAAAPLGRPRTQRTSGITGVVYRTIRGTTRL
                     VSRGVDTSLRLLEPLADEPTVSPKREAALAAINGVWGDHLEASGNPLAIPMSMCTDGR
                     RIELTADGLRAALPAVTGRISVLVHGLCMNDLQWRRDGHHHGTMLERELGYTVLALRY
                     NSGLHVSDNGERFAALLEELIAQWPRPVEELVIVGHSMGGLVARSACRVAETRSLAWR
                     RHFTRLVCLGTPHHGTVLERGGHLLDTVLELSPYVAPFTRLGKARSAGITDLRFGNVQ
                     RADWEGRHPHDQAHDDRTPTPLPAGVQVYLLAATTAQQPRGLRHAIIGDGLVTLASAW
                     GEHRDPAFALNVPPSHKGLVTQASHWDLLSRPEVVDALRCWLD"
     gene            complement(1666..1938)
                     /locus_tag="AC16HB_07465"
     CDS             complement(1666..1938)
                     /locus_tag="AC16HB_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606667.1"
                     /translation="MVRKSAVASKPIFHAPMRLDWTDEKLQTLSQEQLLNLLENLDRQ
                     RTIGRLSEHIAAAFDQRISALLTGRNGTRRRKQVSEDAEVVSAEAS"
     gene            complement(1963..>2385)
                     /locus_tag="AC16HB_07470"
     CDS             complement(1963..>2385)
                     /locus_tag="AC16HB_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606668.1"
                     /translation="EVVWAILADFGTYRRWNPLIRGVLGRPAMGRQIEIQLRDPSGAD
                     IAARLTIVGIREDREMKWQERWTVPGLFASERRFWIEPLTHGVVRFHHGEQVRGIMVP
                     LLRQRRRLRGRPGLDAMNTALKQRAERAWGNRAPTTAA"
BASE COUNT          396 a          824 c          746 g          419 t
ORIGIN      
        1 gccgattcgc caggcgcctt cgaggaggac gtcatgccct tcgaaaccgc ggccaaagcg
       61 atcttcgtca aagccaacgg tctgccggcc tactggaaaa caaccggatt ggtgccgcat
      121 gggaagaaat gctgaatacg gacgacagag cgctcgtccc gaaccccgac cggcgcgcat
      181 ttcacccgcg ggccgcctgc cactgtccct cgccgatctt cgcggcctga ctcgtctggg
      241 tttcgatgcc accgtcggcg tgaccgatct ggtggagcag atgcaccgta cggtcggcga
      301 cgctgccgct ccgctgggcc gccctcgtac ccaacggacc agcggcatca ccggcgtcgt
      361 gtaccgcacg atccgcggca cgactcgcct tgtctcacgc ggtgtcgaca catcgctgcg
      421 tttgctggag ccgctggcgg acgaacccac cgtctcgcca aagcgcgagg cggcgcttgc
      481 cgcgatcaac ggagtgtggg gcgatcatct cgaggcgagc ggcaatcccc tcgcgattcc
      541 gatgtcgatg tgcacggacg gcaggcgcat cgagctcacg gccgatggcc tgcgggccgc
      601 cctccctgct gtcaccggcc gcatctccgt gctggtgcac ggtctgtgca tgaacgattt
      661 gcagtggcga cgcgacgggc atcaccatgg cacgatgctc gagcgcgagc tcggctacac
      721 cgtgctcgct ctgcgctaca acagcggcct gcatgtttcc gacaacggcg agcgctttgc
      781 ggccttgctc gaagaactga ttgcgcagtg gccccggccg gtcgaagagt tggtcatcgt
      841 cggccacagc atgggcggcc tggtggcccg cagtgcctgc cgcgtggccg agacccgatc
      901 actggcgtgg cgcagacact tcacgcggct tgtctgcctg ggaacgccac accacggcac
      961 ggtgctcgag cgcggcggcc atctgctcga cacggttctg gaactgagcc cctacgtggc
     1021 gcccttcacg cgcctgggca aggcacgcag cgcgggcatc accgacctgc gcttcggcaa
     1081 cgtccagcgc gccgactggg aaggccgtca tccgcacgat caggcgcacg acgatcgaac
     1141 gccgacaccg ctgccggccg gcgtgcaggt ctatctgctc gccgccacca cggcgcagca
     1201 gccgcgcggg ttacggcacg ccatcatcgg tgacggcctg gtgacgctcg caagcgcctg
     1261 gggagagcat cgcgacccgg cgttcgcgct gaacgtgccg ccatcgcaca agggcctggt
     1321 cacgcaggcg agccactggg atctgctcag ccgcccggag gttgtcgacg cgttgcggtg
     1381 ctggctggat tgacgtgcgc cgtggcctgc aacagtcgct ccgccggctg ttctcaccgt
     1441 gtcgccgcca cgacgtcaat caaatcagca cgccttcgtg atgtccactc tttcgggaca
     1501 agttcgagac atcgtttccg cttcttcccc acaactcccg cggattgccg accgtgctgc
     1561 ggagatgcct tcgcgccatg gcggcagtga cacgatcgtc gaacgaaatg gtctgtgcat
     1621 agcactggcc tgcgagccga tacaaccagg tgcgtcgacg cacgatcatg acgcttccgc
     1681 ggacaccact tccgcgtcct ccgaaacctg cttacggcgc cgggtgccgt tacggcccgt
     1741 cagcaacgca gagatgcgct gatcgaatgc cgctgcgatg tgctccgaaa ggcggccgat
     1801 cgtccgctgg cgatccaggt tctcgagcag gttgagcaat tgttcctgac tgagcgtctg
     1861 cagtttctcg tcggtccagt caaggcgcat cggcgcatgg aagatcggct tgctggcaac
     1921 cgctgacttg cgcaccatgg actgtccttt cgtgtgaatg ccctaggcgg cagtggtggg
     1981 cgcccggttt ccccacgcgc gttccgcgcg ttgcttcaaa gccgtgttca tcgcgtcaag
     2041 gccaggcctg ccgcgcaacc tgcgccgctg acgcagcagc ggcaccatga ttccgcgcac
     2101 ttgctcgccg tgatggaatc gaacgacgcc gtgcgtaagc ggttcgatcc agaacctgcg
     2161 ctccgaagca aacaggcctg gcaccgtcca gcgttcctgc cacttcatct cccgatcttc
     2221 gcgtatgccc acgatcgtca gacgcgccgc gatgtccgcg cccgacggat ccctgagttg
     2281 gatctcgatc tgtctgccca tcgcggggcg accgagcaca ccgcggatca gcggattcca
     2341 gcgtcggtag gttccgaagt cggcaaggat ggcccagacc acctc
//
LOCUS       DBNHBY010000307         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1236741, whole genome shotgun sequence.
ACCESSION   DBNHBY010000307 DBNHBY010000000
VERSION     DBNHBY010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1236741"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="AC16HB_07475"
     CDS             <1..684
                     /locus_tag="AC16HB_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate:acetyl-CoA acetyltransferase"
                     /protein_id="HIR5606669.1"
                     /translation="VRNDDESPTSSPERFAQVLAGIRQHCPGMITQVSTGGRSGAGRE
                     RGGMLSLRPDMASLATGSVNFPTRVYDNPPDLIDWLAAEMRRYGVKPEVEAFDLSMIF
                     SAVALQKAGKIDAPLHVQFVMGVKNAMPVDRAVFEFYVQTLARLAPGATWTGAGIGKD
                     QITLARWSLELGGHCRTGMEDNVRFDRDTLAPSNAALVRQVANLCEDYGRRPATPAEA
                     RKLLSLLPA"
     gene            complement(821..1582)
                     /locus_tag="AC16HB_07480"
     CDS             complement(821..1582)
                     /locus_tag="AC16HB_07480"
                     /inference="COORDINATES: protein motif:HMM:NF039450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HIR5606670.1"
                     /translation="MRRLGAAIVLAAAAAAAAATPRLDVDGTEFVVTLPDGRQLRSAD
                     LVGATLKVDAGGRPVEIVIEGVEDDRHAVGGKVALHSLLVKDETGRSLPLCKPDPQGR
                     NLAFPVPDGRGGFDLTCTSGAVGKCIRWGYRPWDERPGGPPLKALHQACVHMTRADYG
                     GDGEAHTRNGTLIAVQDRFGIQTFDGETSMAFEAAWGVDGAICVARPRIADVVTLQQL
                     AERYPKLRAALGTQACTEEDASRNPAALLINRSFR"
     gene            complement(1586..>1850)
                     /locus_tag="AC16HB_07485"
     CDS             complement(1586..>1850)
                     /locus_tag="AC16HB_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606671.1"
                     /translation="ATAAHAAAGARDGGECPTNGVYENGIALDGLALQNLRLDRAELI
                     GVRDARSDKVAMNGIWSNGVALQGRQLEALPLRNVQIVLPAAK"
BASE COUNT          265 a          654 c          658 g          273 t
ORIGIN      
        1 gtgcgcaacg acgacgagtc gccgacgtcg tctcccgagc gcttcgcaca ggtgctcgcg
       61 ggcatccgcc agcactgtcc cggcatgatc acgcaggtgt cgaccggcgg ccgttccggt
      121 gcgggcaggg agcgcggcgg catgctgtcg ttgcggccgg acatggcatc gctcgcgacc
      181 ggctcggtca acttcccgac ccgcgtgtac gacaatccgc cggatctcat cgactggctc
      241 gccgcggaga tgcgccgcta cggcgtgaag cccgaggtcg aggcgttcga cctgtcgatg
      301 atcttctccg cggtcgcgct gcagaaggcg ggcaagatcg acgcgccgct gcacgtgcag
      361 ttcgtgatgg gcgtgaagaa cgcgatgccc gtcgatcgcg cggtgttcga gttctacgtg
      421 cagacgctgg cgcggctggc gcccggcgcg acctggaccg gcgccggcat cggcaaggac
      481 cagatcacgc tcgcccgctg gtcgctcgaa ctcggcggcc actgccgcac cggcatggag
      541 gacaacgtgc ggttcgatcg cgacacgctc gcgccgtcga acgcggcgct cgtcaggcag
      601 gtcgcgaatc tgtgcgagga ctacggccgc cgccccgcga cgccggccga ggcacgcaag
      661 ctgctgtcgc tgctaccggc gtgaccggtc ctggagcgag ccggcgcggg cgaaaggagt
      721 cggtccgcgc tggcgcgttg gatcggacgg tccgaggaaa agacgcgcgg ctgcgtgccg
      781 aatcacgttg tcacgccgaa ccgaccgaca ccgaacgccg tcaccggaac gatcgattga
      841 tcagcagcgc cgccggattg cggctggcgt cctcttcagt gcacgcctgc gtgcccagcg
      901 cggcgcgcag cttcgggtag cgctccgcga gttgctggag cgtcaccacg tcggcgatgc
      961 gcggacgagc gacgcagatc gcgccatcga cgccccacgc ggcctcgaac gccatcgaag
     1021 tctcgccgtc gaacgtctgg atgccgaagc ggtcctgcac ggcgatgagc gtgccgttgc
     1081 gcgtgtgcgc ctcgccgtcg ccgccgtagt cggcgcgcgt catgtgcacg caggcctgat
     1141 ggagcgcctt gagcggcggg ccgccgggcc gttcgtccca cggccgatag ccccagcgga
     1201 tgcacttgcc gacggcgccg ctggtgcacg tgaggtcgaa cccgccgcgg ccgtcgggca
     1261 ccgggaacgc caggttgcgc ccctgggggt ccggtttgca gagcggcagc gagcggcccg
     1321 tttcgtcctt gacgagcagg ctgtgcaacg cgaccttgcc gccgaccgca tgacggtcgt
     1381 cctcgacgcc ttcgatcacg atctcgaccg gccggccacc ggcatcgacc ttgagcgtcg
     1441 cgccgaccag gtcggcgctg cgcagttggc ggccgtcggg cagcgtgacg acgaactcag
     1501 tgccgtcgac atcgagacgc ggtgtcgcgg ccgccgccgc agcagcggcg gcaagcacga
     1561 tcgcggcgcc gaggcggcgc atcgcctact tcgccgcggg cagcacgatc tgcacgttgc
     1621 gcagcggcag cgcttcgagc tgccggccct gcaatgcgac gccgttggac cagatgccgt
     1681 tcatcgcgac cttatcgctg cgggcgtcac ggacgccgat cagctcggcg cggtcgagcc
     1741 gcaggttctg cagcgccagg ccgtccagcg cgatgccgtt ttcatagacg ccgttcgtgg
     1801 ggcattcccc gccgtcccgg gctccagccg ccgcatgcgc ggcagtcgcg
//
LOCUS       DBNHBY010000308         2118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1642348, whole genome shotgun sequence.
ACCESSION   DBNHBY010000308 DBNHBY010000000
VERSION     DBNHBY010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1642348"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>2118)
                     /locus_tag="AC16HB_07490"
     CDS             complement(19..>2118)
                     /locus_tag="AC16HB_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606672.1"
                     /translation="VPPTVSSWLDACDAEALERLELRPLTLAATEALLLSLALPQLDA
                     ATWAAPLYRHTGGNPMFILETLLAIAAGAPTTSAVIRLPVPTNIGALIEKRLAQLSPP
                     ALKLARVAALAGPDFSADLAAQVLGVHALDLAEPWRELETALVIRDNAFAHDLIFEAT
                     LRSVPQQIAQLMNRAIAGFLEAHGAPAARVAQHWADAREWSRAGRAFAAAALDARRVS
                     QRAAEVGYWQRACECYEQDGAHDPAFDARRESVEALILIQGVERATEVVDALLREART
                     DAQRVAALTAYAQLRLMAADHAAGVEAAREAYETARLLDSPWPRFEAARLLAIGLSQQ
                     ERATEALPLIEPFRELVEREGSREQRGKFWADYAYALNSARRLRDTADALTQAIAEAR
                     ALGDLGELATLTSNLALVQGNLGRVGEALEQAMRARALSGQLGEAGGPPIGAIDMYVG
                     MYSAMLGRYRDALASLDAALDVFARDRQTQWLAVASNHKAAVLLDLGQAARAQKALEY
                     ELPSIDSVRARRATLAARIERFLGRSGERQIRTALAELGERGDRYVRMLAQLDEAATL
                     PAERGVASCAEVRSVAEELEYAGVAVKARLWGARHLLRTGDAAASAADVHQLLPHLDG
                     VRPADMYFPEAWWIAFEVFEGNQEPAAATAALKRGFDWIHDVALPNVPDEFRDSFLDR
                     NPVNRAIRTTASRRLRC"
BASE COUNT          301 a          773 c          731 g          313 t
ORIGIN      
        1 ggcgggcgat accgttgcct agcagcgcag ccgccggctc gccgtggtgc ggatcgcgcg
       61 gttgaccggg ttgcggtcga gaaagctgtc gcggaattcg tccgggacgt tgggcagcgc
      121 gacatcgtgg atccagtcga agccccgctt cagcgccgcg gttgcagcgg ccggctcctg
      181 gttcccctcg aacacctcga acgcaatcca ccacgcctcg gggaagtaca tgtcggccgg
      241 gcgcacgccg tccaggtgcg gcagcagctg atggacgtcg gccgcactcg ctgccgcatc
      301 gccggtgcgc agcagatgcc gcgcgcccca taagcgcgcc ttgaccgcca caccagcgta
      361 ttcgagctcc tcggccacgc ttcgcacttc ggcacacgac gcgacccccc gctccgccgg
      421 cagagtcgct gcctcgtcga gctgcgccag catgcggaca tatcggtcgc cgcgttcgcc
      481 gagttccgcc agcgcggtgc ggatctggcg ctcgccggag cgtccgagaa agcgctcgat
      541 gcgcgctgcg agcgtcgccc gtcgcgcacg gaccgaatcg atcgatggca gctcgtattc
      601 gagcgccttc tgcgcccgcg ccgcctgtcc gaggtcgagc agcacggcgg ccttgtgatt
      661 gctcgccacc gcgagccact gcgtctgacg gtcgcgcgcg aatacgtcga gcgccgcgtc
      721 gaggctcgcc agcgcgtcgc ggtagcggcc gagcatcgcg ctgtacatgc cgacgtacat
      781 gtcgatcgct ccgatcggcg gcccacccgc ctcgcccaat tgcccgctca gtgcgcgcgc
      841 gcgcattgcc tgttcgagcg cttcgccgac gcggccgaga ttgccctgca ccagcgcgag
      901 gttggaggtc agcgtcgcca gttcgccgag gtcgccgagc gcgcgcgcct cggcgatggc
      961 ctgcgtcagc gcatccgccg tgtcgcgcag ccggcgggcc gagttcagcg catacgcata
     1021 gtcagcccag aacttaccgc gctgttcgcg gctgccctcg cgctcgacca gttcgcgaaa
     1081 gggctcgatc agcggcagcg cttcggtggc acgctcctgt tgcgacaggc cgatcgccag
     1141 caggcgcgcc gcctcgaagc gcggccacgg cgaatcgaga aggcgtgccg tttcataggc
     1201 ctcgcgcgcc gcttccacgc cggccgcgtg atcggccgcc atcagccgca gctgcgcgta
     1261 agcggtgagc gcggcgacgc gctgcgcatc ggtccgagcc tcgcgcagca gggcgtcgac
     1321 gacctcggtc gcgcgctcga caccctggat caggatcaac gcttcgacac tctcgcgccg
     1381 cgcgtcgaac gcggggtcgt gcgcgccgtc ctgctcatag cattcgcatg cgcgctgcca
     1441 gtacccgacc tcggccgcgc gctgcgacac gcggcgcgca tcgagcgcgg cggccgcaaa
     1501 ggcgcgaccg gcgcgactcc actcgcgtgc gtcggcccaa tgctgcgcga cgcgcgcggc
     1561 gggtgcgccg tgcgcctcga ggaagcccgc gatcgcgcgg ttcatcagct gcgcgatctg
     1621 ctgcggcacc gagcgtagcg tggcttcgaa gatcaggtcg tgcgcgaatg cattgtcgcg
     1681 gatgaccagc gcggtttcga gttcgcgcca gggctcggcc agatcgagcg cgtgcacgcc
     1741 cagcacctgt gcggccaggt ccgcgctgaa gtccgggccg gccagcgcgg ccacgcgcgc
     1801 cagcttgagc gccggcggcg acaattgcgc cagacgcttc tcgatgaggg ccccgatatt
     1861 ggtcggcacc ggcaggcgga tcacggcact cgtggtcggc gcgcccgcgg caatcgcaag
     1921 cagcgtctcc agaatgaaca tcggattgcc tccggtgtgc cggtacagcg gcgccgccca
     1981 ggtcgccgca tcgagctgcg gcaatgccag cgagagcagt agcgcttccg ttgccgcgag
     2041 cgtcaagggg cgcagttcga gccgctccaa cgcttcggcg tcgcaggcgt cgagccagct
     2101 gctcacggtc ggcggcac
//
LOCUS       DBNHBY010000309         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_966045, whole genome shotgun sequence.
ACCESSION   DBNHBY010000309 DBNHBY010000000
VERSION     DBNHBY010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_966045"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16HB_07495"
     CDS             <1..423
                     /locus_tag="AC16HB_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HIR5606673.1"
                     /translation="DLRRARAAAHNMIPTKTGAAAAVGLVLPELNGKLDGYAVRVPTI
                     NVSIVDLSFVAARDTTVDEVNSIMKAAAEGQLKGILGYNADPLVSSDFNHDPLSSVFD
                     ATLTKVSGRLVKVSSWYDNEWGFSNRMLDVTMALMAAK"
     gene            715..1014
                     /locus_tag="AC16HB_07500"
     CDS             715..1014
                     /locus_tag="AC16HB_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606674.1"
                     /translation="MLLTVCAALGACASMASESARVIVTLAPGVEISDPGAFERTVRE
                     RTSVRVTYAAAVSERMHALHVHCETSDAGCAHAQEVLRASGLFSSLTEDQRRKRQ"
     gene            1040..>1626
                     /locus_tag="AC16HB_07505"
     CDS             1040..>1626
                     /locus_tag="AC16HB_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606675.1"
                     /translation="MMSRRVLCGAVALIATFILSAPAQAERTTRAARADAGTDRIIVK
                     FRATAPRAQSAADEAAATDRVAALAARTGVRLQVARSVAPQMHAVKLDRVLAGAELQD
                     MIARIGRDSAVEYAVADGRKYRALLPNDPLYGTAPVTQQWWLTPPSSTFVSPIDAEHA
                     WDITTGSPGVVVAVIDSGVLYDHPDLGRVAQGGKIL"
BASE COUNT          261 a          579 c          543 g          243 t
ORIGIN      
        1 gatctgcggc gcgcgcgcgc ggccgcgcac aacatgattc cgaccaagac cggcgccgcc
       61 gctgcggtcg gcctggtgct gccggagctg aacggcaagc tcgacggtta cgcggtgcgc
      121 gtgccgacga tcaacgtttc gatcgtcgac ctgtcgttcg ttgccgcgcg cgacaccacg
      181 gtcgacgagg tcaactcgat catgaaggcg gccgccgaag gccagctgaa aggcatcctc
      241 ggctataacg ccgacccgct cgtatccagc gacttcaacc atgacccgct gtcatccgtg
      301 ttcgacgcca ccctgaccaa ggtctccgga cgtctcgtca aggtctcgag ctggtatgac
      361 aatgaatggg gcttctccaa ccgcatgctg gacgtcacga tggcgctgat ggcggcaaag
      421 tgacgcggcc gccgaagccg agccccgttc attgcgtcgc cggctgactc gcgcgcagcg
      481 caagtcaagg gccgctggcc cggccgggaa cgcaacgagc ggggttccag caaccgcatc
      541 ctgtacgcca ccgtggcccg ggcggcggac cggtcaggcc gaccgcgaag tcgtgcccca
      601 tccatctcgt cgatacatcg ttttgttaca tcgatgggcg ccgccgctcg ctacactggc
      661 cgcgtgtcag ccgatcacgc agccccacct gcgcgaagat tccgtgccgc caacgtgctg
      721 ctgacggtct gcgcggccct tggcgcctgc gcctccatgg cctccgaatc tgcgcgcgtg
      781 atcgtcaccc tggcccccgg cgtcgagatc tccgatcccg gggcgttcga gcgcacggtg
      841 cgcgaacgga cgtccgtgcg ggtcacctat gccgcggccg tgtccgagcg catgcacgcg
      901 ctgcatgtcc attgcgaaac gagcgacgcc ggctgcgcgc acgcgcagga agtgctgcgg
      961 gcatcggggc tgttctcttc gctcaccgaa gatcagcgcc gaaagcgcca gtgaatcgcc
     1021 ccagggagag cgacgaacca tgatgtccag acgagtgctg tgcggcgcgg tcgcgctgat
     1081 cgcgacgttc atcctgtcgg cgcccgcgca ggccgagcgc acgacgcgcg cagcgcgcgc
     1141 ggacgccggg accgaccgca tcatcgtgaa gttccgcgcc accgcgccgc gcgcgcagtc
     1201 ggccgcggac gaagcggccg cgacggatcg cgtcgcggca ctcgccgccc gcaccggcgt
     1261 gcgtctgcag gtggcgcgca gcgttgcgcc gcagatgcac gcggtcaagc tcgaccgcgt
     1321 gctcgccggc gccgaattgc aggacatgat cgcgcgcatc ggtcgcgaca gcgcagtcga
     1381 atacgcagtg gccgacggtc gcaagtaccg cgcgctgctg ccgaacgatc cgctatatgg
     1441 gaccgctccg gtgacgcagc agtggtggct gacgccgccc agcagcacgt tcgtctcgcc
     1501 gatcgatgcg gagcacgcgt gggacatcac caccggcagc ccgggcgtcg tcgtcgccgt
     1561 gatcgacagc ggcgtgctgt acgaccaccc ggacctcggc cgtgtcgcgc agggcggcaa
     1621 gatcct
//
LOCUS       DBNHBY010000310         8772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_153525, whole genome shotgun sequence.
ACCESSION   DBNHBY010000310 DBNHBY010000000
VERSION     DBNHBY010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_153525"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..470
                     /gene="rplS"
                     /locus_tag="AC16HB_07510"
     CDS             96..470
                     /gene="rplS"
                     /locus_tag="AC16HB_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5606676.1"
                     /translation="MDLIQQLEKEEVARVTANKTIPEFGPGDTVVVNVNVVEGSRKRV
                     QAYEGVVIARRSRGLNSSFVVRKISSGEGVERTFQLYSPSIASIEVKRRGDVRRAKLY
                     YLRDRSGKSARIREKLATRGEE"
     gene            511..1245
                     /locus_tag="AC16HB_07515"
     CDS             511..1245
                     /locus_tag="AC16HB_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HIR5606677.1"
                     /translation="MSELKPHPDPTAPPRLILDPEAVPLDVAFADAAARGSAVAPERL
                     RPVALRSRLAAPPAWRPEMTGDEKLALLTRPPRPASVLIPLVVRDQNISVLLTKRTSH
                     LHDHAGQISFPGGRADEGDADAIATALRESREEIGLVPDVVDVIGTMPHYVTITGYRV
                     TPVVALIEHEFSLTLDDFEVEEAFEVPLTFLMDPANHERRRIEVGGISRAFYAMPYAD
                     SRAAKRYFIWGATAAMLRNFYHLLAA"
     gene            1265..2221
                     /locus_tag="AC16HB_07520"
     CDS             1265..2221
                     /locus_tag="AC16HB_07520"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HIR5606678.1"
                     /translation="MGWLALLLVLLLEQVRPVPARNPVYAGAAWIADGAERNLNAGSP
                     RHGLYAWLLVVGGATIAAGILHALALSVSWVVALALNLVVLYFTLGFRQFSHPFTEIQ
                     IALANGDLATARRELTAWKSQSDEGYSAADLDASEVARQAIEYGIVLAHRHVFGVFFW
                     FVVLPGPMGPVFFRLADYVARRWNRRTRAPLPADEFGGFARKAFAWVDWLPARMTALG
                     FAIVGDFEGAVYCWRKVKGVGGFPGVESRAVVLGAASGALGTRIMSAPESARYFDEPG
                     QEGAGLREPDSNTPRAVVALAWRAMILWLIVLLALSLAGWLA"
     gene            complement(2225..2530)
                     /locus_tag="AC16HB_07525"
     CDS             complement(2225..2530)
                     /locus_tag="AC16HB_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR5606679.1"
                     /translation="MKLLIRCDQTLQAAHIANALRAAGVVCEVRNTTLSSAIGDIPFL
                     ECAPQVWLVNDLDEPRARMLLRELMQPVSGPTWRCAKCGEELEPQFGACWACGAPRP"
     gene            complement(2527..3423)
                     /gene="rsgA"
                     /locus_tag="AC16HB_07530"
     CDS             complement(2527..3423)
                     /gene="rsgA"
                     /locus_tag="AC16HB_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR5606680.1"
                     /translation="MNEPRADTAERARVVASFGRHHLIDSGGALLHAVRRGKRGDVIV
                     GDLVSCQRTGTDQAVIETVLPRASLLYRADRKRTKEMAANIDQLAIVFAAQPAFNIRF
                     VWRALAAAHAAAVGSMVILNKIDLPDLEAAQARLGQVKALGYRSLAISARRDPEAARA
                     TLLPLLAQRATLLIGQSGMGKSTLLNLLVPGAKAMTQEFSHHLSLGKQTTTSSRWLAL
                     PEGGALVDSPGFQAFGLSHLDDAAIVASLPEFAAHLGQCRFLDCRHLAEPDCAIRAAV
                     DAGRIDAQRYDFYRELLAERAP"
     gene            complement(3420..3770)
                     /locus_tag="AC16HB_07535"
     CDS             complement(3420..3770)
                     /locus_tag="AC16HB_07535"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR5606681.1"
                     /translation="MVARAELLQQHCRERKGSALGDAQVQTQLVALPDWSLREGQLVR
                     TYRFGNYYETIAFVNALAFMVHREDHHPDLVVGYNRCEVRFSTHSVGGISDNDFICAA
                     KADAIFAQQFGVAA"
     gene            complement(3774..5039)
                     /locus_tag="AC16HB_07540"
     CDS             complement(3774..5039)
                     /locus_tag="AC16HB_07540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5606682.1"
                     /translation="MPTATHFTFIFVALLLLMLGLKYWLALRQIRHVAAHANAVPAQF
                     ADRVSLDAHRKAASYTIAKQRLGLIETAVGAALLVALTLLGGIDLIAGGLAQLVGRGF
                     AFQILIVAAVAVVGAALDLPFEWYRQFSLEQRYGFNRMTPRLFLTDLSKSIALAIVLG
                     LPLLAVVLWLMDKAGAMWWFYAWLVWAGFSLLILVLYPTLIAPLFNKFEPLRDVMLAE
                     RVQALLTRTGFSSRGVFVMDGSRRSSHGNAYFTGLGRAKRVVFFDTLVQRLNAPEIEA
                     VLAHELGHFKLKHIAKRLIVTFASSLALLALLGWLANQTWFYVGLGVTPALEARNDGL
                     AIVLFMLVLPVFTFVLAPLASLFSRKHEFEADAFAARHASAADLIAALVKLYEDNAAT
                     LTPDPVHSAFYDSHPPATIRIQRLLGAAG"
     gene            5107..5715
                     /gene="orn"
                     /locus_tag="AC16HB_07545"
     CDS             5107..5715
                     /gene="orn"
                     /locus_tag="AC16HB_07545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR5606683.1"
                     /translation="MADEKIIPQAGSGSAAPGARSDRFDDNNIVWVDMEMSGLNPDSD
                     RIIEIAVVVTDSQLQVIAEGPVLAIHQSDAIIDAMDEWNRTTHGRSGLTERVRASTID
                     EAQAEHDVVEFLSRYVPPRKSPMAGNSICQDLRFMARWMPQLEAYFHYRNLDVSSLKE
                     LARRWAPGVNRSFEKKSRHEALADIYESIEELRHYRVQMMKC"
     gene            complement(5801..5872)
                     /locus_tag="AC16HB_07550"
     tRNA            complement(5801..5872)
                     /locus_tag="AC16HB_07550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5837..5839),aa:Arg,seq:cct)
     gene            6039..8030
                     /locus_tag="AC16HB_07555"
     CDS             6039..8030
                     /locus_tag="AC16HB_07555"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR5606684.1"
                     /translation="MTTVSRPRSRRASAEYGEASIRVLRGLEPVKERPGMYTRTDSPL
                     HIVQEVVDNAADEILSGFGAHIDVIVHADGSVSVEDDGRGIPVGLHPEEQAPVVEIVF
                     TRLHAGGKFDKRAGGAYSFSGGLHGVGVSVTNALASRLDVTVWRDGKVHTIGFADGDV
                     KEPLRSRARKAGDKATGTRVTVWPNPKYFDTATVPVDELERLLRSKAVLLPGTEVRFA
                     IEKSGVEQRWRYSGGLQEYLLACIGAEPLLPPFESEGYAAPDDQTFAEGEGARWVLAW
                     TEEGPVARESYVNLIPTPAGGTHESGLREGMFNAVRAFIEAHSLLPKGVKLLPEDVYA
                     RASFVLTCKVLDPQFQGQIKERLNSRDAVRLVALFIKPQLEFWLNQNPEYGRRLADLV
                     IRQAQARARASQKVEKKKGSAVAVLPGKLTDCESTDIERNEVFLVEGDSAGGSAKMGR
                     DKEFQAILPLRGKVLNTWEVERDRLFANAEVHDISVAVGIDPHGAGDEVDFSALRYGK
                     VCILADADVDGAHIQVLLLTLFFRHFPRLLERGHVYVARPPLFRVDVPAHGRRPARRI
                     YCLDDAELDTTLEKLVDEGVRDGSWSIARFKGLGEMSAEQLWETTMNPDTRRLLPIGL
                     GEVDLESTRSTMTMLMGRTEAQSRRTWIEEHGDEVEADV"
     gene            complement(8127..8603)
                     /locus_tag="AC16HB_07560"
     CDS             complement(8127..8603)
                     /locus_tag="AC16HB_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606685.1"
                     /translation="MKNKRHAFLWIAALFIAQCAWAQDKTGDGTDLQALKLAVTKDKK
                     GLVASMLDLTPAESKKFWPLYDAYQRSVDAANRRRTTALEGVIGMNKPMTDLFARNVA
                     NELTTADETEVKARRTLQNRVLRALPAKKAARYLQLESKIRAMQAYDIAAAIPLIR"
BASE COUNT         1468 a         2873 c         2987 g         1444 t
ORIGIN      
        1 ggtccggaac tgaaggtccc atcctctggg gcggcgacaa cggcggaacc ttccgcgcac
       61 aacagtcgtc acacgatggt cattggagag tgaagatgga cttgatccag cagcttgaga
      121 aagaagaggt cgcccgcgtc accgcgaaca agacgatccc cgaattcggt cccggcgaca
      181 ccgtcgtcgt caacgtcaac gtcgtcgagg gcagccgcaa gcgcgtgcag gcgtacgaag
      241 gtgttgtgat cgccaggcgc agccgggggc tcaacagttc gttcgtggtg cgcaagatct
      301 ccagcggcga gggtgtggag cggacgttcc agctgtactc gccgtcgatc gcctcgatcg
      361 aggtcaagcg ccgcggcgac gtgcgtcgcg ccaagctgta ctacctgcgc gaccgctccg
      421 gcaaatcggc ccgcatccgc gaaaagctcg caacgcgggg cgaagagtag gttgctccgg
      481 cggcgccgtt gcaccggcgc cgctcctcgc atgtcggagc tcaagccgca ccccgatccg
      541 accgcgccgc cgcgcctgat cctcgatccc gaggcggtgc cgctcgatgt tgcctttgcg
      601 gacgctgcgg cgcgcggtag cgcagtggcc cccgagcggt tgcggccggt cgcgctgcgc
      661 tcgcggctgg ctgcgcctcc cgcgtggcgt cccgagatga ccggtgacga gaagctcgcc
      721 ctgctcaccc ggccgccccg gccggcgtcg gtgttgatcc cgctggtcgt gcgcgaccaa
      781 aacatctcgg tgttgctcac gaagcgaacc tcgcacctgc atgatcacgc gggccagatc
      841 agctttccgg gcgggcgagc cgacgaaggc gacgccgatg cgattgccac cgcactgcgc
      901 gaatcgcgcg aagagatcgg actcgtcccc gacgttgtcg acgtgatcgg cacgatgccg
      961 cactacgtga cgatcaccgg ctaccgcgtc accccggtcg tggcgctcat cgagcacgag
     1021 ttcagcctca cgctcgacga cttcgaggtc gaagaggcct tcgaggtgcc gctcacgttc
     1081 ctgatggacc ccgccaacca tgagcggcgc cgcatcgagg tcggtggcat cagccgggcg
     1141 ttctacgcga tgccgtatgc tgattcgcgc gcggcgaagc gctacttcat ctggggcgcg
     1201 accgcggcga tgctgcgcaa cttctaccac ctgctcgccg cctaactcaa atccggagat
     1261 cgacgtgggc tggctcgcgc tgctgctggt gttgctgctc gagcaggtgc gtccggtacc
     1321 ggcgcgcaac ccggtctacg ccggcgccgc ctggatcgcg gacggcgcag agcggaatct
     1381 gaacgccggc tcgccgcggc acggcctgta tgcctggctg ctcgtggtcg gcggcgcaac
     1441 gatcgccgcg ggaatcctgc acgcgctcgc cctgtcggtt tcctgggtcg tggcgctggc
     1501 gctcaatctc gtcgtgctgt atttcacgct cggctttcgc cagttcagcc atccgttcac
     1561 cgagatccag atcgcgctcg ccaacggcga tctcgcgacg gcccggcgcg aactgaccgc
     1621 gtggaagagc cagtcagatg agggctacag cgccgcggat ctcgatgcct ccgaagttgc
     1681 gcggcaggcg atcgagtacg gcatcgtgct cgcgcaccga cacgtcttcg gcgtcttctt
     1741 ctggttcgtc gtgttgccgg gcccgatggg tccggtgttc tttcggcttg ccgactacgt
     1801 ggcgcggcgc tggaaccggc gcacgcgggc gccattgccg gccgacgaat tcggcggctt
     1861 cgcacgcaag gcattcgcat gggtcgactg gctgccggcg cggatgaccg cgctcggttt
     1921 cgcgatcgtc ggcgatttcg aaggcgcggt gtactgctgg cgcaaggtca agggtgtcgg
     1981 cggcttcccg ggcgtcgagt cgcgcgccgt cgtgctcggc gccgccagcg gtgcgctcgg
     2041 cacgcgcatc atgagcgcgc cggagtcggc gcgctacttc gacgagccgg gccaggaggg
     2101 ggcggggctg cgtgagcccg attcgaacac gccgcgcgcg gtggtcgcgc tggcgtggcg
     2161 ggcgatgatc ctgtggctga tcgtgctgct cgcgttgtcg ctggcgggct ggctggcctg
     2221 atcgttacgg acgcggcgcg ccgcacgccc agcacgcgcc gaactgcggt tcgagttcct
     2281 cgccgcactt cgcgcagcgc caggtcggac cgctgaccgg ctgcatcagt tcgcgcagca
     2341 gcatccgcgc ccgtggttcg tcgaggtcgt tgacgagcca cacctgcggg gcgcattcga
     2401 ggaatggaat gtcaccgatc gcgctcgaaa gcgtcgtgtt gcgcacctcg cagacgacgc
     2461 cggccgcgcg cagcgcgttg gcaatgtgcg cggcttgcag cgtctggtcg cagcgaatca
     2521 gcagcttcat ggtgcgcgct ccgccagcag ctcgcgatag aagtcatagc gctgcgcatc
     2581 gatgcgtccg gcatcgaccg ctgcgcggat cgcgcagtcg ggctcggcga ggtgccggca
     2641 gtcgagaaag cgacactgac ccagatgcgc agcgaactcc ggcaacgacg cgacgatcgc
     2701 ggcgtcatcg agatgcgaca gcccgaacgc ctggaacccc ggcgaatcga cgagcgcgcc
     2761 accttcgggc agcgcgagcc agcgcgacga cgtggtcgtc tgcttgccga ggctcaggtg
     2821 gtgagagaat tcctgcgtca tcgccttcgc gccgggcacc agcaggttga gcagcgtcga
     2881 cttgcccatg ccggactggc cgatcagcag cgtggcgcgc tgcgcaagca acggcagcag
     2941 cgtcgcgcgc gccgcctccg gatcgcgccg cgcgctgatc gcgagcgagc ggtaaccaag
     3001 tgccttcacc tgcccgagcc gcgcctgcgc tgcttcgaga tccggcaggt cgatcttgtt
     3061 caggatcacc atgctgccga ccgctgcggc gtgcgcggcc gcgagcgcac gccacacgaa
     3121 tcggatgttg aacgctggct gggcggcgaa cacgatggcc aactgatcga tgttggcggc
     3181 catctccttg gtgcgtttgc ggtctgcccg atagaggagg ctggcgcgcg gcagcacggt
     3241 ttcgatcacg gcctggtccg tgccggtgcg ctgacaagac accaggtcgc cgacgatcac
     3301 gtcgccgcgc ttgccacgtc gtaccgcgtg cagcaatgcg ccgccgctgt cgatcaagtg
     3361 gtgcctgccg aaacttgcca cgacgcgcgc ccgttccgcg gtgtcggcgc gcggctcgtt
     3421 catgcagcga cgccgaactg ctgcgcaaag atcgcgtcgg ccttcgccgc gcagatgaag
     3481 tcgttgtccg aaatgccgcc gacggaatgc gtgctgaagc gcacttcgca gcggttgtac
     3541 ccgacgacga ggtccgggtg atgatcctcg cgatggacca tgaacgcgag cgcgttgacg
     3601 aaagcgatgg tctcgtaata gttgccgaac cgataggtgc gaaccagctg tccttcgcgc
     3661 aggctccagt ccggcagcgc gaccagctga gtctgcacct gcgcgtcgcc gagcgcgctg
     3721 cccttgcgct cccggcaatg ctgctgcaag agctctgcac gcgcgaccat cgttcatcct
     3781 gcagcgccga gcagccgctg gatgcggatc gtcgccggcg gatgcgagtc gtagaacgcc
     3841 gagtgcacag ggtcgggcgt cagtgtcgcc gcgttgtcct cgtacagctt gaccagcgca
     3901 gcgatcagat cggcggccga cgcgtggcgg gcggcgaacg cgtcggcctc gaactcgtgc
     3961 ttgcgcgaga aaaggctcgc cagcggcgcc agcacgaacg tgaagaccgg cagcacgagc
     4021 atgaacagca cgatggccag accgtcgttg cgggcctcga gcgccggcgt cacgccaagc
     4081 cctacgtaga accacgtctg gttcgcaagc cagccgagca gcgccagcag tgcgaggctg
     4141 gatgcgaacg tgacgatcag gcgcttggcg atgtgcttca acttgaaatg cccgagctcg
     4201 tgtgccagca cggcctcgat ttccggcgcg ttcagccgct gcaccagcgt gtcgaagaac
     4261 accacgcgct tggcgcgtcc cagcccggtg aaatacgcgt tgccgtggct ggagcggcgc
     4321 gagccgtcca tcacaaagac gccgcggctg gagaagccgg tgcgcgtcag cagcgcctgc
     4381 acgcgctcgg cgagcatgac gtcccgcagc ggttcgaact tgttgaaaag cggcgcgatc
     4441 agcgtcggat acagcactag gatcagcagg ctgaagcccg cccacaccag ccacgcgtag
     4501 aaccaccaca ttgcgccggc cttgtccatc agccacaaca cgaccgccag cagcggcagg
     4561 ccgagcacga tcgcgagcgc gatcgacttg gacaggtcgg tcaggaacag ccgcggcgtc
     4621 atgcggttga agccgtagcg ctgctcgagt gagaactgcc ggtaccactc gaacggaagg
     4681 tcgagtgcag cgccgacgac tgcgaccgcg gcgacgatca ggatctggaa tgcgaagcca
     4741 cggccgacca gttgcgccaa tccgcccgcg atcaggtcga tcccgccgag cagcgtcagt
     4801 gcgaccagca gcgcggcgcc aacggccgtc tcgatcagtc cgagccgctg cttggcgatc
     4861 gtgtatgacg ccgctttgcg gtgcgcgtcg aggctgacgc gatcggcgaa ctgcgccggc
     4921 accgcattcg catgcgcggc gacgtggcgg atctgccgca gcgccagcca gtacttcagg
     4981 cccagcatca gcagcagcag ggcgacgaag atgaacgtga aatgcgtcgc agtcggcata
     5041 cgggtatcgg cctggcacgg ttcgcggtcg cgtgcgtgcg acaatcgcaa gtgacgaaga
     5101 ctggctatgg ccgacgagaa aattatccca caggcaggct ccggaagcgc tgcgccgggt
     5161 gcgcgctcgg atcgcttcga cgacaacaat atcgtctggg tcgacatgga aatgagcggg
     5221 ctgaacccgg attcggatcg catcatcgag atcgcggttg tcgtcaccga ctcgcagttg
     5281 caggtgatcg ccgagggccc ggtgcttgcg atccaccagt ccgacgcgat catcgacgcg
     5341 atggacgagt ggaaccgcac cacgcacggt cgctccgggc tcaccgagcg cgtgcgcgcg
     5401 tcgacgatcg acgaggcgca ggccgagcac gacgtggtcg agttcctgtc ccggtatgtt
     5461 ccgccgcgca aatcgccgat ggcgggcaac tcgatctgcc aggacctgcg cttcatggcg
     5521 cgctggatgc cgcagctcga agcgtacttt cactaccgca atctcgacgt cagctcactg
     5581 aaggagctgg cgcgccgctg ggcgccgggc gtgaatcggt cgttcgagaa gaagagccgg
     5641 cacgaggcgc tcgccgacat ctacgagtcg atcgaggaac tgaggcacta tcgggtgcag
     5701 atgatgaagt gctgaagagc gcccacgcgc gggcgtcgac gttcgttcgc gaccccgccc
     5761 agctcgctgc gcatgccaac gcgcttgccg gcgcgatccg tcccgccgcc aggaatcgaa
     5821 cctggatctg agtcttagga ggaccctgtt ctatccattg aactacggcg agcgcgcgca
     5881 gtatacggaa cgctctcgta gaatccgcgc ggccgattgc agtcgtgcgc agcgagcggg
     5941 caggccggtg cggaccgcat gaactcttgc ttcgcgcgct ctgacgccgc acttttccac
     6001 aacttctgtg gataacccga tttcctcttg acattcgcat gacgacagtg tctcgcccgc
     6061 gttcccgccg tgccagcgca gagtacggcg aagcctcgat ccgcgtgctg cgcggcctcg
     6121 agccggtcaa ggagcggcct ggcatgtaca cgcgcaccga cagcccgttg cacatcgtgc
     6181 aggaggtggt cgacaacgct gccgacgaga tcctgtccgg cttcggcgcg catatcgacg
     6241 tcatcgtgca tgccgacggc tcggtcagcg tcgaggatga cggccgcggg attccggtcg
     6301 gactgcatcc cgaagagcag gcgccggtgg tcgagatcgt cttcacacga ctgcacgccg
     6361 gcggcaagtt cgacaagcgc gcaggtggtg cttattcgtt ctccggcggg ctgcatggcg
     6421 tcggcgtgtc ggtgaccaac gcgcttgcct cgagactcga tgtgaccgtg tggcgcgacg
     6481 gcaaggtgca cacgatcggt ttcgccgatg gcgacgtgaa ggagccgctg cgctcgcgag
     6541 cccgcaaggc gggcgacaag gcgaccggca cgcgcgtgac cgtctggccg aatccgaagt
     6601 acttcgacac cgcgaccgtg ccggtcgacg aactcgagcg gctcctgcgc agcaaggcgg
     6661 tgctgctgcc cgggaccgag gttcggtttg cgatcgagaa gtcaggcgtc gagcagcgct
     6721 ggcgctacag cggcggcctg caggaatatc tgctggcatg catcggcgcc gagcctctgc
     6781 tgccgccgtt cgaatccgaa gggtatgcgg cgcccgacga ccagacgttc gccgagggcg
     6841 agggcgcgcg ctgggtgctg gcgtggaccg aggaggggcc ggtcgcgcgc gagtcgtacg
     6901 tgaacctgat cccgacgccg gccggcggca cgcacgagtc cggtctgcgc gagggcatgt
     6961 tcaacgcagt gcgtgcattc atcgaagcgc acagcctgct gcccaagggt gtaaagctgc
     7021 tacccgagga cgtgtacgcg cgtgccagct ttgtactgac gtgcaaggtg ctcgatccgc
     7081 agtttcaggg acagatcaag gagcggctga acagccgcga cgcggtgcga ctggtcgcgt
     7141 tgttcatcaa gccgcaactg gagttctggc tcaaccagaa tccggaatat ggccgcaggc
     7201 tcgcggatct cgtgatccgg caggcgcagg cgcgcgcgcg cgcgtcgcag aaagtcgaga
     7261 agaagaaggg ctcggcggtc gcggtgctgc cgggcaagct gaccgactgc gagtcgaccg
     7321 acatcgagcg caacgaggta ttcctcgtcg aaggcgattc cgccggcggc tcggcgaaaa
     7381 tgggccgcga caaggagttc caggcgatcc tgccgctgcg cggcaaggtg ctgaacacgt
     7441 gggaagtcga gcgcgaccgg ctgttcgcca atgccgaggt gcacgacatc tcggtggcag
     7501 tcggtatcga cccgcacggc gctggcgacg aggtcgactt ctccgcatta cgctacggca
     7561 aggtgtgcat cctcgccgac gcggacgtcg acggggcgca catccaggtg ctgctgctga
     7621 cgctgttctt ccgccacttt ccgcgcctgc tcgagcgcgg ccacgtgtac gtcgcgcggc
     7681 cgccgctgtt ccgcgtcgat gtccccgcac atggcaggcg accggcgcgg cggatctact
     7741 gcctcgacga tgccgagctc gacacgacgc tggagaagct cgtcgacgag ggcgtgcgcg
     7801 acggcagctg gagcattgcg cgcttcaagg gcctgggcga gatgagcgcc gagcagctgt
     7861 gggaaacgac gatgaatccc gacacgcgcc ggctgctgcc gatcgggcta ggtgaagtcg
     7921 atctggagag cacgcgctcg acgatgacga tgctgatggg ccgcaccgaa gcgcagagcc
     7981 gtcgcacctg gatcgaggag cacggcgacg aggtcgaggc ggacgtctga gagcaacgcg
     8041 cgcagcgacc gcgcggcgac tgccgagcag gaaactgcgc cgacgccctt gctccctgac
     8101 cgactcagcg agccgacggg cagcaactat ctgatcagcg gaatcgcggc cgcgatgtca
     8161 taggcctgca tcgcacggat tttcgattcg agctgcaggt agcgcgccgc cttcttcgcc
     8221 ggcagcgctc tcagcacgcg gttctgcagg gtccggcgtg ccttgacctc ggtttcgtcg
     8281 gccgtggtca gttcattggc gacattgcgc gcgaataggt cggtcatcgg cttgttcatt
     8341 ccgatcacgc cttcgagcgc cgtcgtgcga cgccggttgg ccgcgtcgac gctgcgctga
     8401 tacgcgtcgt acagcggcca gaacttcttc gactcggccg gtgtcagatc gagcatcgat
     8461 gccaccagtc ccttcttgtc cttggtgacg gcgagcttca gcgcctgcag gtcggtcccg
     8521 tcgccggtct tgtcctgcgc ccaggcgcac tgcgcgatga aaagcgccgc gatccagaga
     8581 aatgcgtgtc tcttgttctt catgtctcct ccgtctcgtc agttgcgtgg cgcccatgcc
     8641 gctcgcctcg gatcggcgcc cgatgcgcga cggaatctac gctgtttctg cgcggtggca
     8701 atgcagtcgc cacgccacgg ttccggtgcg caggcggcta cgagtcgccg ctaacgaacg
     8761 gactggcttt tc
//
LOCUS       DBNHBY010000311         5303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_627303, whole genome shotgun sequence.
ACCESSION   DBNHBY010000311 DBNHBY010000000
VERSION     DBNHBY010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_627303"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="AC16HB_07565"
     CDS             <1..2131
                     /locus_tag="AC16HB_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HIR5606686.1"
                     /translation="EVLGRIITRPRAKNLPSISADNLTLVETDEWVSIAVIDRVGGRM
                     LGALDNVLRDLHVVVGYLAGRESLRTVDNPFSPAVFLHALIGAADDLGAHSDAAEPLI
                     AAFDRPLADELPKIYAAVLDHLRAHGLDAKAIRRELAARQATAFKGADAAAAMRAKDA
                     AMRGPDTSMRGPDTLGLAAVPDTQGPITQGPAAPPVVGLQLPINAAAALGNLMVRLQA
                     NARGEPLPRGLQSPGVAPPQLLEAVQEFQHLGLQGLAGAAITTAEGEAGAWREHLINK
                     ASRTVDKLTIEIVGMLFDHVLQDKQVPVEIKALISRLQFPILKVALLDAAFFASGNHP
                     ARRLIDRIASTSIGWEPYGDENEKYRAEVERVVRTVLERFDKDTTIFERLLGEFDLFV
                     GETSTRDNDPVARAKRALEEAEKREILVINTTIQVRRAFEKVELEPYLRDFLVGPWVQ
                     ALVNSILRDDQTPGFSKAFRQVIHDLVWSVQPKVSADERKRLVALIPPTMRIIRDGLN
                     SIRMPEHEQNQFFQQLMQSHAMAVRPVDQATYIKHSLATTELRSRIDEMRITGTFPIT
                     TVAGGVRVSEGALLRAAEQHAAQVMIPVAPTDVGPIDRDEEADLDEKIATWQRGTWFD
                     LWNGTEFIRARLRWISPLRTLFMFSCAQEAQARVLAPDVLKSYLKRGFLKPLEHEPLM
                     KRAVDGVVSEFEKMPKRAEELAARFAA"
     gene            2176..2325
                     /locus_tag="AC16HB_07570"
     CDS             2176..2325
                     /locus_tag="AC16HB_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606687.1"
                     /translation="MSALERLVASAKGAAQDNMHSLRLPGLEKFAQGVTTLDEVLRPA
                     PPFET"
     gene            complement(2350..3468)
                     /locus_tag="AC16HB_07575"
     CDS             complement(2350..3468)
                     /locus_tag="AC16HB_07575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR5606688.1"
                     /translation="MSRGPSRIERVREFLSTPARMVVALLVAIFAVEFAVMATLGALA
                     ANLPWWMLTLIDTAVTTTLVSLFMWLAMIAPLRQALEHERAKAQIVLDHAAEAIITID
                     RTGTIRAFNRAAERMFGYGAADVIGTHINLIVPQSQRSEHDAHLRRYATDGQSEMLGK
                     RRQVQAQRRDGTTLPVEMSMSEAKVDGERLITAILLDITERKRLEERIGRLAHFDNLT
                     GLPNRASFLEQLDRALALAHRQQHKVGLLFLDLNGFKPVNDALGHEAGDQLLKLVAAR
                     LSAQARESDFVARLGGDEFTVILPLVQGRAAADDAAQRIAGAFGQPFDLGSGLVPLSA
                     SVGIAIYPDDAQVARDLIRIADTRMYDAKGSRKPVRTT"
     gene            complement(3465..4637)
                     /locus_tag="AC16HB_07580"
     CDS             complement(3465..4637)
                     /locus_tag="AC16HB_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5606689.1"
                     /translation="MLINFFYHLRAAKLPVSIKELLTLLEALKQDVIRPSIDDFYFLS
                     RAALVKDEAQFDKFDRAFGEFFNGVRTTLGFSKEIPDEWFRQLLERDFSPEEIAKLEK
                     LGFDKLLEEFRKRLEEQDGAHHGGNKWIGSGGTSPFGHGGAHPEGIRIGGPSKGGRSA
                     VKVWEARAYRDYDDQVELGTRNIKVALRRLRRFAREGADLELDLPDTISSTARNAGWL
                     DIKMVPERHNTVKVLMLLDVGGTMDEHIKGVEELFSAAKAEFKHLEFYYFHNCVYEYV
                     WRNNRRRFAEKFDTWDVLRKYNPDYKLIFVGDATMSPYEILQPGGSVEHNNAEAGAVW
                     LQRIAHHFPKHIWINPEPEGLWQYRQSIAVISQILAGRMFPLTLEGLARAMRQLSK"
     gene            complement(4642..5235)
                     /locus_tag="AC16HB_07585"
     CDS             complement(4642..5235)
                     /locus_tag="AC16HB_07585"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606690.1"
                     /translation="MIEPRRVTLEGHGVRLEPLAREHHDGLAAAAADGGLWKLWFTSV
                     PAPEETHAYISAALAGQEAGHMLPWAVRDLTSNTIVGSTRYHDIVPAIDRVEIGYTWY
                     ARRCQKTHVNTACKLLLMTNAFETLGCKVVGFRTDNFNFASQAAIAALGAKKDGVLRH
                     HQARRDGTVRDTVLFSILAAEWHDVRRHLELRLARHG"
BASE COUNT          838 a         1770 c         1795 g          900 t
ORIGIN      
        1 cgaggtgctc ggccgcatca tcacgcgccc acgcgcgaag aacctgccga gcatctcggc
       61 cgacaacctg acgctggtcg agaccgacga gtgggtgtcg atcgcggtca tcgatcgtgt
      121 cggcggcagg atgctaggtg cgctcgacaa cgtgctgcgc gacctgcacg tggtggtcgg
      181 ctatctggcg ggccgcgagt cgctgcgtac cgtcgacaac ccgttttcgc ccgcggtatt
      241 cctgcatgcg ctgatcggcg ctgccgacga cctgggcgcg cattcggacg cggccgagcc
      301 gctgatcgcc gctttcgacc gtccgctggc agacgaattg ccgaagatct atgcggccgt
      361 gctcgaccac ctgcgcgcgc acggattgga cgcgaaggcg atccggcgcg agctcgcggc
      421 gcggcaggca actgcgttca agggcgccga cgcagccgca gcgatgcgcg caaaggacgc
      481 ggcgatgcgc ggcccggata cgtcgatgcg aggccccgac acgctcgggc ttgccgccgt
      541 gccggataca cagggcccga tcacgcaggg cccggccgcc ccgcccgtgg tgggactgca
      601 gcttccgatc aatgccgccg ccgcgctcgg caacctgatg gtgcggctgc aggccaacgc
      661 gcgcggcgaa ccgctcccca ggggactgca atcgcccggg gtggcaccgc cgcagctgct
      721 cgaagcggta caggagttcc agcaccttgg cctgcagggg ctggccggcg cggcgatcac
      781 gacggccgag ggcgaagccg gcgcgtggcg cgagcacctg atcaacaagg cgagccgtac
      841 cgtggacaag ctgacgatcg agatcgtcgg catgctgttc gatcacgtgc tgcaggacaa
      901 gcaggtgccg gtggagatca aggcgttgat ctcgcggctg cagttcccga tattgaaggt
      961 cgcgctgctg gacgccgcct tcttcgcctc cggcaaccat ccggcgcggc gactgatcga
     1021 ccgcatcgcc agcacgtcga tcggctggga gccatacggc gacgagaacg agaagtaccg
     1081 cgccgaagtc gagcgggtgg tgcgcaccgt tctggaaagg ttcgacaagg acacgacgat
     1141 cttcgagcgg ctgctcggcg aattcgatct gttcgtcggc gagacctcga cccgcgacaa
     1201 tgatccggtc gcgcgcgcca agcgcgcgct cgaggaggcg gagaagcgcg agatcctggt
     1261 gatcaacacc acgatccagg tgcggcgtgc gttcgagaag gtggagctcg aaccgtacct
     1321 gcgcgacttc ctggtcggcc cgtgggtgca ggcgctcgtc aactcgatcc tgcgcgacga
     1381 ccagacgccc ggcttctcga aggcgttccg ccaggtgatc cacgacctgg tgtggagcgt
     1441 gcagcccaag gtgtcggccg atgagcgcaa gcggctggtg gcgctgatcc cgccgacgat
     1501 gcgcatcatt cgcgacggcc tgaactcgat tcgcatgccg gagcacgagc agaaccagtt
     1561 cttccagcag ctgatgcagt cgcacgcgat ggcggtacgc ccggtcgacc aggcgaccta
     1621 catcaagcat tcgctcgcga ccaccgaact gcgctcgcgc atcgacgaga tgcgcatcac
     1681 cggcacgttc ccgatcacga cggtcgccgg tggggtcagg gtgtccgagg gtgcgctgct
     1741 gcgcgccgcc gagcagcacg cggcgcaggt gatgattccg gtcgcgccga cggacgtcgg
     1801 cccgatcgac cgcgacgagg aagccgacct cgacgagaag atcgcgacct ggcagcgcgg
     1861 cacctggttc gacctgtgga acggcaccga attcatccgc gcgcggctgc gctggatcag
     1921 cccgctgcgg acgctgttca tgttcagctg cgcgcaggag gcgcaggcgc gcgtgctggc
     1981 gcccgacgtg ctgaagtcgt acttgaagcg ggggttcctg aagccgctcg agcatgagcc
     2041 gttgatgaag cgcgccgtcg acggcgtggt cagcgaattc gagaagatgc ccaagcgcgc
     2101 cgaggagctc gcggcgcgct tcgcggccta ggccgtgcgc gctggcgcgc tgccggcgtc
     2161 cccgctccgc ggtcgatgag cgcgctcgag cgcttggtcg cgtccgccaa gggcgccgcg
     2221 caggacaaca tgcattcgtt gcgcttgccg ggtttggaga agttcgcgca gggcgtcacc
     2281 acgctcgacg aagtgctgcg ccccgcgccg ccgttcgaga cctgacttaa ggcgccgctg
     2341 ctcgcggtgc tatgtcgttc gcaccggttt gcgcgagccc ttggcgtcgt acatccgcgt
     2401 gtcggcgatg cgaatcaaat ccctggcgac ctgtgcgtcg tcggggtaaa tggcaatgcc
     2461 cacgctcgcg ctcagcggca ccaggcccga tccgaggtcg aatggctgac cgaaggcacc
     2521 ggcgatccgc tgcgccgcgt cgtccgctgc ggcacgcccc tgcaccaggg gcaggatgac
     2581 ggtgaattcg tctccgccca gacgcgcgac gaaatcggat tcgcgtgcct gcgcgctcag
     2641 gcgtgccgcg acgagcttca gcagctgatc tcccgcctcg tggccgagcg cgtcgttgac
     2701 cggtttgaac ccattcagat cgaggaagag cagcccgacc ttgtgctgct ggcggtgcgc
     2761 gagcgccagt gcgcggtcca gctgctccag gaacgacgcg cgattgggca gccccgtcag
     2821 attgtcgaag tgcgccagtc ggccgatgcg ttcctcgagc cgcttgcgct ctgtgatgtc
     2881 gagcaggatc gccgtgatca gccgctcgcc gtcgaccttc gcctcgctca tcgacatctc
     2941 gacggggagc gtggtgccgt cccggcgctg cgcctgaacc tggcgccgct tgccgagcat
     3001 ctcgctctgt ccgtcggtcg cgtaccgccg caggtgcgcg tcatgctccg accgctgcga
     3061 ctgcggcacg atcaggttga tgtgggtgcc gatcacgtcg gcggcgccgt atccgaacat
     3121 gcgctcggcg gcgcgattga aggcgcggat cgtgcccgta cggtcgatcg tgatgatggc
     3181 ctcggctgca tggtcgagga cgatctgtgc cttggcgcgt tcgtgttcca gcgcctgtcg
     3241 cagcggcgcg atcatggcca gccacatgaa cagcgacacc agcgtcgtcg tcacggcggt
     3301 gtcgatcagg gtcagcatcc accatggcag gttcgccgcc agcgcgccga gcgtggccat
     3361 gacggcgaac tcgaccgcaa agatcgcgac cagcagcgcg acaaccatgc gcgccggtgt
     3421 cgacaggaat tcccgcacgc gctcgatgcg cgacggcccg cggctcactt ggacagctgg
     3481 cgcatcgcgc gcgcgagtcc ttcaagtgtc agcgggaaca tgcgaccggc caggatctgg
     3541 ctgatcaccg cgatcgactg ccggtactgc cacaggccct cgggctcggg gttgatccag
     3601 atgtgtttcg ggaaatggtg agcgatgcgc tgcagccata ccgcgcccgc ctcggcattg
     3661 ttgtgctcga cgctgccgcc cggctgcagg atttcgtatg ggctcatcgt cgcgtcgccg
     3721 acgaagatca gcttgtagtc ggggttgtac ttgcgcagca cgtcccaggt gtcgaacttc
     3781 tccgcgaagc ggcgccggtt gttgcgccaa acgtactcgt acacgcagtt gtggaagtag
     3841 tagaactcga ggtgcttgaa ctcggccttc gctgcggaga acagctcctc gacgcccttg
     3901 atgtgctcgt ccatcgtgcc gccgacgtcg agcagcatca gtaccttcac cgtgttgtgc
     3961 cgctctggca ccatcttgat gtcgagccag ccggcgttgc gcgcggtcga cgagatcgtg
     4021 tcgggcaggt cgagctcgag gtccgcgccc tcacgggcga agcggcgcag ccgccgcagc
     4081 gcgaccttga tgttgcgtgt gccgagttcg acctgatcgt cgtaatcgcg atacgcgcgc
     4141 gcttcccaca ccttgacggc cgagcgtccg cccttgctcg ggccgccgat gcgaatgcct
     4201 tccggatgcg cgccgccatg accgaacggg gaggtgccgc cgctgccgat ccacttgttg
     4261 ccgccgtggt gcgcgccgtc ctgttcctcg agccgcttgc ggaattcctc cagcagcttg
     4321 tcgaagccga gcttctccag cttggcgatc tcctccggcg aaaagtcgcg ctccagcagt
     4381 tgtctgaacc actcgtccgg gatctccttc gagaagccga gcgtggtcct gacgccgttg
     4441 aagaattcgc cgaacgcgcg atcgaacttg tcgaattgcg cctcgtcctt cactagcgcg
     4501 gcgcgcgaca ggaagtagaa gtcgtcgatc gacggtcgga tcacgtcctg tttcagcgct
     4561 tccagcagcg tcagcagctc cttgatcgac accggcagct tcgccgcgcg caggtggtag
     4621 aagaagttga tcagcatcgc ctcacccgtg gcgcgccagg cgcagttcga gatggcgcct
     4681 cacgtcgtgc cattcggccg ccaggatgct gaacagcacc gtgtcgcgca ccgtgccgtc
     4741 gcgccgcgcc tgatgatgcc gcagcacgcc gtccttcttc gcgccgagcg cggcgatggc
     4801 ggcctgcgag gcgaagttga agttgtcggt gcgaaagccg accaccttgc agccgagcgt
     4861 ctcgaaggcg ttcgtcatca gcagcagctt gcacgcggta ttcacgtgcg tcttctggca
     4921 gcggcgcgcg taccaggtgt agccgatctc gacgcggtcg atcgcgggca cgatgtcgtg
     4981 atagcgcgtc gagccgacga tggtgttgct cgtcaggtcg cgcaccgccc acggcagcat
     5041 gtggcccgct tcctgccccg ccagcgcggc cgagatgtac gcgtgcgttt cttcgggcgc
     5101 cggcaccgac gtgaaccaca gcttccacag tccgccgtcc gcggcggcgg cggcgagccc
     5161 gtcgtggtgc tcccgcgcca gcggctcgag ccgcacccca tgcccttcga gggtgacgcg
     5221 ccgcggttcg atcacggcgg ctaccggttg tggcgggcca tgaagacgag ccgctcgaac
     5281 agatgcatgt cctgctcgtt ctt
//
LOCUS       DBNHBY010000312         2854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_289544, whole genome shotgun sequence.
ACCESSION   DBNHBY010000312 DBNHBY010000000
VERSION     DBNHBY010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_289544"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..497)
                     /locus_tag="AC16HB_07590"
     CDS             complement(75..497)
                     /locus_tag="AC16HB_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606691.1"
                     /translation="MSSAFENLCGPGKPLKAEARDAKELAGLVRSGTTRLKDAGTKGL
                     ALESRFDLAYNAAHALCLAALRWHGYRSSNRYVIFQLLPQTLGLGPEVWRVLAKCHEI
                     RNLGEYEGNLDIDDRIVKDLIVACTAVAAKVAALPAPN"
     gene            complement(481..1065)
                     /locus_tag="AC16HB_07595"
     CDS             complement(481..1065)
                     /locus_tag="AC16HB_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5606692.1"
                     /translation="MSLADALFSGTQQRVLGLLFGNLRRSFYATELIALAGAGSGAVQ
                     RELARLEHSALVTVRRVGNQKHYQANAESPLFLELCGIVQKTVGLADPLREALRPLSA
                     RIIAAFVFGSVAKREDTAASDIDLMVVSDSLTYADVFAAVEKAGDRLGRVVNPTILSR
                     KALTKRAKEDNAFVTRVLSQPKVWLIGDEHVLGL"
     gene            1363..2565
                     /locus_tag="AC16HB_07600"
     CDS             1363..2565
                     /locus_tag="AC16HB_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606693.1"
                     /translation="MTTLPLVSSGGAAASLLEPTPHLDQNSASNSALDEQFVAHGTDA
                     GDALARFGAPHDLHPLAQQQLSYLSNGKAHAANSKQAACSVPDASTRTYDIARTSQAD
                     IDALKASCDPAQRRLGYTIENAKASYGDLLAKGARIVVTLNAGNGGEPVMTLMGPGFD
                     PKLPARIHTHYHGDNATVGDPVGSKAGQNSRIRETVARDPQTVFVLPECKSVFDKDTP
                     PDSPTHDKSYHADWSNVQSQARTTDDALKAAGITKIEKETVSVHSRGGEVIQKLMHDD
                     PSGKSLRADRLELHDSLYGSQGAVAAWGRTENGKQVEKVIYVHGTNADGRDKEIAKTF
                     KGAYVRIDISHQRPLNDKSNPVVYDGKDAARDDHIHGKKGHEGARQFHPNAHYQTTGR
                     FLAIWPLP"
BASE COUNT          569 a          957 c          855 g          473 t
ORIGIN      
        1 gtgtcgcgtc gatcgcgaca caccctgccg cttcgacaca tcgagtcggg agctttcgct
       61 ccgctatcgc tgtatcaatt tggcgccggc aaagcagcga cctttgccgc aactgccgtg
      121 cacgccacga tgagatcctt cacgatccga tcgtcgatgt cgagattgcc ttcgtactcg
      181 ccaagatttc gaatctcgtg acacttggcg agcacgcgcc agacttccgg ccccaggccg
      241 agcgtttgcg gtaatagttg gaagatgacg tagcgatttg aagaccgata accgtgccag
      301 cgcagcgccg cgagacacaa tgcatgcgcc gcgttgtagg cgaggtcgaa ccggctctcc
      361 agcgccagcc ccttcgtacc ggcgtccttc aggcgcgtag tgccggagcg caccaacccc
      421 gcaagctcct tagcgtcccg cgcctcggcc ttcaacggct tgcctggacc gcacagattc
      481 tcaaaggccg aggacatgtt catctccgat cagccacacc ttcggctgcg aaagcacccg
      541 agtgacgaag gcattgtctt cctttgcgcg cttcgtcaac gccttgcgcg acaagatggt
      601 cggattcacg acgcgtccaa ggcgatcgcc cgccttctcg acggccgcaa agacgtctgc
      661 gtaggtcagt gagtcgctga ccaccatcag gtcgatgtcg ctcgcagccg tgtcttctcg
      721 cttggcgacc gaaccaaaga caaacgcagc gatgattcga gcagaaagcg gccgcagcgc
      781 ttcgcgcagc gggtcggcga gtcccacggt tttctgcaca atgccgcaca gctccaagaa
      841 caacggcgac tcggcattgg cctgatagtg cttctggttg cccacacgac ggaccgtcac
      901 cagagcgctg tgctcgagcc gagccagttc gcgttgcacg gcgccggacc ccgccccggc
      961 caaggcaatc agttcagtgg catagaagct gcgacgtaga ttgccgaaca gcaggccgag
     1021 cacacgttgc tgcgtgcccg agaacaatgc gtcggcgagg ctcaagcgcg actgggctga
     1081 cttcgtcctg ggttgggcgg ttcgtttcat acccatcttg ggcatgatca tacccaaatt
     1141 gggcagattt gctagcggca gaccctcttg tgtttgtact tgcgtgatct gtcgctcgac
     1201 tcattttgcg catggctctc cagaggctgc gcgcttttga tttcaacctg atccccgtcc
     1261 tgaaggttcg ggattcaaca gagaatttga accattaccc gcctgcgccg cactcatcga
     1321 gacgccttcg gccacacggc gggggcaatg ctggagattt ctatgaccac cctgcccctc
     1381 gtgagcagcg gcggcgctgc cgcatcgctg ctcgaaccga ccccgcacct ggaccaaaac
     1441 agcgcatcga actctgcgct ggacgaacag tttgtcgcac acggcaccga tgccggtgac
     1501 gcgctggccc gcttcggcgc gccgcacgat ctgcacccgc tggcccagca gcagttgtcc
     1561 tatctctcga atggaaaggc ccacgcggca aacagcaagc aggccgcctg ctcggtgccc
     1621 gacgcgtcga cccgcaccta cgatattgcc aggacatcgc aagccgacat cgatgcgttg
     1681 aaggcgagct gcgacccggc gcaacggcgc ctgggctaca cgatcgaaaa cgccaaggca
     1741 tcgtatggcg acctgctggc caagggcgcg cgcatcgtcg tgacgctgaa tgccggcaac
     1801 ggcggcgagc ccgtgatgac gctgatggga ccgggcttcg atcccaaact gccggcgcgt
     1861 atccacaccc actaccacgg cgacaacgcc accgtcggcg acccggtcgg ctccaaggcc
     1921 ggtcagaact cgcgcattcg cgagacggtg gcgcgcgatc cgcagacggt gttcgtgctg
     1981 cccgaatgca agagcgtgtt cgacaaagac acgccgccgg attcgccgac ccacgacaag
     2041 tcctaccacg ccgactggag caacgtgcag agccaggccc ggaccaccga cgatgcgctc
     2101 aaggccgccg gcatcacgaa gatcgagaag gaaaccgtgt cggtgcattc gcgcggcggc
     2161 gaggtgatcc agaagctgat gcacgacgat ccgtcgggca agtctctgcg cgccgaccgg
     2221 ctcgagctgc acgactcgct ctacggctcg caaggcgccg tggccgcatg gggccgcacc
     2281 gaaaacggca agcaggtgga gaaagttatc tacgtgcacg gcaccaacgc cgatggccgc
     2341 gacaaggaaa tcgccaagac gttcaagggc gcgtacgtgc gcatcgacat ctcgcaccag
     2401 cggccgctga acgacaagtc gaaccccgtg gtctacgacg gcaaggacgc cgcgcgcgac
     2461 gaccacatcc acggcaagaa gggccacgaa ggcgcgcgcc agttccaccc caacgcgcac
     2521 taccagacca cgggccggtt cctggcgatc tggccgctgc cctgaacgca gagcgccacg
     2581 actgagcgca cggcggctgg caacgtgccg gccccgcggc agcagggatt ctgatcacga
     2641 gaccgccgcg ggtgcgcgcg ccgagacggt cgccggccgc tcggaatttg caaaccctta
     2701 caaagatgac gcccgcgttc acgttgcgct cgtcgatact tcggtctcgg cagaccgagg
     2761 agaacccgac gatgatgaac gtgcttctga cttccctgct ggcgacggtt gtcccgaccg
     2821 accgtcacgt cgacgtgttg gcgcagatga gcgc
//
LOCUS       DBNHBY010000313        11705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_424336, whole genome shotgun sequence.
ACCESSION   DBNHBY010000313 DBNHBY010000000
VERSION     DBNHBY010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_424336"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="AC16HB_07605"
     CDS             complement(<1..1018)
                     /locus_tag="AC16HB_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5606694.1"
                     /translation="MENRNLWLRRGAITLVIVAALAAVVWWIGRPKPIPVVLTEVGRG
                     KVESTIANTRAGEVEACLRTKLSTIAGGRIEVLTVKEGDKVKKGQLLMQLWNDDIQAQ
                     EKVAQAQAETARKRVVEACTLADSAEREVARQGELYAKGFVSINAIDSARAAADAKRA
                     SCQTARADVAQAEANIRLARVQRGRTVLVAPFDGTVAKIVGELGEYSTPSPPGVPTPP
                     AIDLIDESCLYVNAPMDEVDAPKIVAGQPARITLDALPKQAFAGRVKRVAPYVTAVEK
                     QARTVEIEATFDEPEKVGRLLVGYSANVEIVLAARDDVLRVPTAAILEGGRVLVVGSD
                     GRLVE"
     gene            complement(1138..1362)
                     /locus_tag="AC16HB_07610"
     CDS             complement(1138..1362)
                     /locus_tag="AC16HB_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR5606695.1"
                     /translation="MRTIQVKVKPNARASVLEAREDGTWHALLKSPPIEGRANEELVA
                     LVATHFGVRKAQVTIRSGASGRLKLVRIDD"
     gene            complement(1359..1946)
                     /locus_tag="AC16HB_07615"
     CDS             complement(1359..1946)
                     /locus_tag="AC16HB_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR5606696.1"
                     /translation="MTAFATLLSEVRACRHCAGHLPLGPRPVLQAHPQARVLIAAQAP
                     GRRVHASGIPFDDASGERLRDWMGVARDVFYDATRIAILPMGFCYPGAGRSGDRPPRP
                     ECAPLWRERLLRGLKRIELTLVIGQYAHAYHLRNGAALTARVRAWREFAPDVFPLPHP
                     SPRNNGWLRANSWFEVELLPALRARVATVLGRQTR"
     gene            complement(1962..2909)
                     /locus_tag="AC16HB_07620"
     CDS             complement(1962..2909)
                     /locus_tag="AC16HB_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5606697.1"
                     /translation="MTSVARESKVRVAVVQAGSVPFDTGACVDKAMRLTADAAANGAK
                     LVLFPEAFIVGYPKGLNYGLVVGARDPVGREEFRLYLDAAIEVPGPHTQRLGEAAAAH
                     HVYLVVGVIERELGTCYCTVLFFGPDGALLGKHRKLMPTALERMIWGFGDGSTLTVVD
                     SPYGRIGSVICWENYMPMLRMAMYAKNVALYCAPTADDRDTWLASMQHIALEGRCFVL
                     TACQYLRKKDFPASVRVSLGDSPDAVLMRGGSAIVSPLGKVLAGPNFEGETILTAELD
                     LAEIGRGKFDFDAAGHYSRPDVFQLVVNEKPMPAVVAKS"
     gene            3146..4846
                     /locus_tag="AC16HB_07625"
     CDS             3146..4846
                     /locus_tag="AC16HB_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5606698.1"
                     /translation="MEHNISLITTVAVGLGLALVLGFAAVRVRIPALVGYLLAGILIG
                     PATPGFVADVGIASQLSEIGVMLLMFGVGLHFSLGDLLAVKRIAVPGAVVQMGMATVL
                     GMAIAWWWGWSFGHGLLFGVSLSCASTVVLLKALESQGLLESMNGRIAVGWLVVEDLA
                     TVLILVLLPALAPVLGGTNAPAADSVPLWRTIGLTLLQVSAFIAVMLIAGRRVLPWLL
                     WQVAQTGSRELFTLLVVAAAISIAYAAAQLFHVSFALGAFFAGMVMRESEFSHRAAEE
                     SLPLRDAFAVLFFVAVGMLFDPSVLIEEPARVLITVAIIVVGKSIAAMALVLALRYPL
                     NTALTVAASLAQIGEFSFILAGLGMTLGLLSAEAMSLVLAGALISIALNPFVFAAVAP
                     IRNWIIERSELARRLESRGDPYAELPTSTERKYLAGQAVLVGYGRVGQRIAEALQANG
                     IPFVVAEQNREQVESLRAQGIAAVSGDATEPSVLIQAHIADAAMLVVAAPDPVGVRRI
                     VETARALNPDIEVVLRTHGEDESKLLRKEKIGTVFYGEEELARGMTSHVLQRFAPKSA
                     "
     gene            4915..5799
                     /locus_tag="AC16HB_07630"
     CDS             4915..5799
                     /locus_tag="AC16HB_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5606699.1"
                     /translation="MVRLHRLLAATDLSAPARHAAERAALLARHLGASLDLAHVASLA
                     PLEKLRRLVADVPAQLDERVLEVAREEMLELAATLRQRHGIAAEVRVVSGQLLPRLSQ
                     LADAIAADLIVLGARGASFMQHRLLGSSAERLVRRASRPALVVKQTAHEPYRNVLVPV
                     DFSPHSLPALQRARAIAPDAAIALLHACEVPFEGKLRFAGVAEATINHYRESAWAQAL
                     QSMQALCEAAGLPPRSVRVLLLHGDPVQQILQQEQERDCDLIVMGKHGESAIEDLLLG
                     SVTRHVLARSQCDVVVSV"
     gene            complement(5872..6510)
                     /locus_tag="AC16HB_07635"
     CDS             complement(5872..6510)
                     /locus_tag="AC16HB_07635"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR5606700.1"
                     /translation="MQLIGMLDSPYVRRVAVSLQLLGLPFEHRSLSVFRTFDEFSAIN
                     PVVKAPTFVCDDGTVLMDSTLILDYAEHLAAPRSLMPSGLAARTRALRVIGLALAACE
                     KSVQIIYERTLRPAEKQHAPWLERVSGQLLAACAALEAEVARAPLAVTGATIDQAGVS
                     TAVAWHFITQTSVSEIVRADAHPALAEFSARAEALPEFRAAPHGAGTYAGAG"
     gene            complement(6510..7001)
                     /locus_tag="AC16HB_07640"
     CDS             complement(6510..7001)
                     /locus_tag="AC16HB_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5606701.1"
                     /translation="MADDRPFDGGCTCRYVRYRMTTRPLIVHCCHCRWCQRETGASFA
                     LNAVIEADRVQLLSGTVEVIDTPSASGRGQKISRCPRCRVAVWSNYAGGGDAVRFVRV
                     GTLDEPDRMPPDIHIFTASKQPWVVLPPGARAVEAYYDAAEVWSSESLARRAALQARR
                     GGS"
     gene            complement(7079..7396)
                     /locus_tag="AC16HB_07645"
     CDS             complement(7079..7396)
                     /locus_tag="AC16HB_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HIR5606702.1"
                     /translation="MITVIATFGLPQRLGVAEARRIFQSTAPRYRDMPGLVRKYYVLS
                     EDGQTAGGVYLWRSRREAEALYTDAWKAFVREKYGAEPSLAYFDCPVVVDNTTHEIIA
                     DDQ"
     gene            complement(7467..7934)
                     /locus_tag="AC16HB_07650"
     CDS             complement(7467..7934)
                     /locus_tag="AC16HB_07650"
                     /inference="COORDINATES: protein motif:HMM:NF020177.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HIR5606703.1"
                     /translation="MTANIIRFVLLMLLALLVGTMFGVWVGFDPAGLSASAYVEQQQN
                     AIRSLNVLLPAMGAVCIVLAIALAVTARDDARDRTLLAAAAVLMIVAGLVTRFANQPI
                     NAIVMTWSAQAPAANWAQLRDTWWHWHIVRTLAGIAALALVVLATLGAHRAKA"
     gene            complement(7944..8429)
                     /locus_tag="AC16HB_07655"
     CDS             complement(7944..8429)
                     /locus_tag="AC16HB_07655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606704.1"
                     /translation="MTAPSARVTLREITSETVIPVVKLAVAESQKGFVAPNAVSLAQA
                     LFSDEAWYRAIYVDEEPAGFVMLADESLRTPPPEQPKVGVWRLMVDERFQGRGVGRAA
                     LMQVIEHVRAKGVFAALELSFVPGPGSPEPFYLGLGFRHTGRVDGNEVVLELPLRADL
                     G"
     gene            complement(8466..9011)
                     /locus_tag="AC16HB_07660"
     CDS             complement(8466..9011)
                     /locus_tag="AC16HB_07660"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR5606705.1"
                     /translation="MKRYVALLRGVNVGRANRLAMADLRRVLESLGHRNVRTLLASGN
                     AVFDGGSAAPPVQAERIRAALARTLDIDVPVLVKPAADIAAAVAENALAGVATDAARL
                     LVAFTRDAASLAQLGALRALVRENERLQIGAHAAYLWCPEGIVKSKAGAALLGKPPAE
                     TTTRNWATVLKLADLTRERAA"
     gene            complement(9192..10292)
                     /locus_tag="AC16HB_07665"
     CDS             complement(9192..10292)
                     /locus_tag="AC16HB_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HIR5606706.1"
                     /translation="MTTHGGHASQATGAGANARGLGGLDGLSPAYFGMVMATGIVSLA
                     AHMLDLPRLAQVLFALNIAAYAVLWLLTLLRAARYPRHFFTDMVDHLRGPGFFTTVAG
                     TSVLGSQFVVLAAGDRAGVALWGVAIVLWIGFTYTIIAGLTIKAHKPALAEGISGAWL
                     LAVVATQSIAVLSALLAPHFGQPGRLEINFLALSMWLWGGMLYIWMMSLIFYRYTFFT
                     LAPGDLSPPYWINMGAMAISTLAGSLLIINSPDAPFLLSLLPFIKGFTVFYWATGTWW
                     IPMLLILALWRHVYRRFPLRYDPLYWGAVFPLGMYAASTHEMIEAMSFDFLRFLPSVF
                     LYVALAAWLAAFAGLVLDLLRRAGVLRAKERA"
     gene            complement(10289..10915)
                     /locus_tag="AC16HB_07670"
     CDS             complement(10289..10915)
                     /locus_tag="AC16HB_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5606707.1"
                     /translation="MLEVTWLLFIFASIVLIVTPGQDMMLVMSRSIAQGSAAGVATAA
                     GVSVGLVGHTILATLGLGAILRTSEWLFVALKFAGAAYLVYLGVQLLRSGRHEFAVAA
                     GTPRTLRRLFFDGAFSNISNPKIAVFYFAFLPQFVLPHAAHPTLSVFALGLAFAGLTF
                     LVKGPVGFGAGFLSGWLRSRPDALAWLYRTSGVVLVGLGLKLAFERRA"
     gene            complement(11015..11422)
                     /locus_tag="AC16HB_07675"
     CDS             complement(11015..11422)
                     /locus_tag="AC16HB_07675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5606708.1"
                     /translation="MHGVESLQALLAPLLPGLLDIALKETTADRVVGTMQVRPDLCTT
                     GNTLHGGAVMAFADTLGAVGTFLNLPAGARTTTVESSTKFIGGAPVGTAVVGESTPFH
                     KGRTTMVWQTIVRAESGKLCAVVTQTQLILPAR"
     gene            complement(11424..>11705)
                     /locus_tag="AC16HB_07680"
     CDS             complement(11424..>11705)
                     /locus_tag="AC16HB_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5606709.1"
                     /translation="PPQVTIGPDDAIGPRETRGANVAGTQVHAVRLPGHVLGVDVHFG
                     MPDQTLTLRHRAGASARPYVDGALIAIRKVTGLVGVHRGLDRVLDLQGG"
BASE COUNT         1838 a         4133 c         3951 g         1783 t
ORIGIN      
        1 gctcgaccag ccggccgtcg ctgccgacga cgagcacacg gccgccttcg aggatcgccg
       61 cggtgggcac gcgcagcacg tcgtcgcgcg cagcgagcac gatctcgacg ttcgcgctgt
      121 agccgacgag caggcgtccg accttctccg gctcgtcgaa cgtggcctcg atctcgaccg
      181 tgcgcgcctg cttctcgacc gcggtcacgt acggcgccac gcgcttgacc ctgccggcga
      241 acgcctgctt cggcagcgca tcgagcgtga tgcgcgcggg ctggccggcg acgatcttcg
      301 gcgcgtcgac ttcgtccatc ggcgcgttca cgtacaggca cgactcgtcg atcaggtcga
      361 tcgcgggcgg tgtcggcact cccggcggcg acggcgtcga gtattcgccg agttcgccga
      421 cgatcttcgc caccgtgccg tcgaacggcg ccaccagcac cgtgcggccg cgctgcacgc
      481 gcgcgagcct gatgttcgct tcggcctgcg ccacgtcggc gcgcgccgtc tggcacgacg
      541 cacgcttcgc atcggcggcg gcgcgcgccg agtcgatggc gttgatcgac acgaagcctt
      601 tcgcatagag ctcgccctgg cgcgccactt cgcgctcggc ggaatcggcc agcgtgcacg
      661 cctcgaccac gcgcttgcgc gcggtctccg cctgcgcctg cgcgaccttc tcctgcgcct
      721 ggatgtcgtc gttccaaagc tgcatcagga gctggccctt cttgaccttg tcgccttcct
      781 tcacggtcag cacctcgatg cgcccgccgg cgatcgtcga cagcttggta cgcaggcacg
      841 cctcgacctc gcccgcgcgc gtgttggcga tcgtcgattc gaccttgccg cggccgacct
      901 cggtcagcac caccgggatc ggcttcggcc gcccgatcca ccacaccacg gcggcgagcg
      961 cggcgacgat caccagtgta atggcgccgc ggcgcagcca gaggtttcgg ttttccatga
     1021 ggggagttcg ggatcgaagg ctgggccagc gtagccgcgc gcgggcattg cgacattgac
     1081 gcggcacagg atgctacgcg agcagcgtcg ccctcgccgc cggacctgcg cagcgattca
     1141 atcgtcgata cggacgagct tcaatcgccc gctggcaccg ctcctgatcg tcacctgtgc
     1201 cttgcgcacg ccgaagtgcg tggcgacgag cgcgaccagt tcctcgttcg cgcggccctc
     1261 gatcggcggc gacttcagca gcgcgtgcca ggtgccatcc tcccgcgcct cgagcacgga
     1321 cgcgcgcgcg ttcggcttga ccttgacctg gatcgttctc accgcgtttg gcgcccaagg
     1381 acggtcgcaa cccgcgcccg cagcgcgggc agaagctcga cctcgaacca tgaattggcg
     1441 cgcagccagc cgttgttgcg cgggctcgga tgaggcagcg ggaacacgtc gggcgcgaat
     1501 tcccgccacg cgcgcacgcg cgcggtcagc gctgcgccat tgcgcaggtg atacgcgtgc
     1561 gcgtattgcc cgatcacgag cgtgagttcg atgcgcttca ggccacgcag cagccgctcg
     1621 cgccacagcg gcgcgcactc gggccgcggc ggccggtcgc ccgaacggcc cgcgcccgga
     1681 tagcagaagc ccatcggcag gatcgcgatc ctcgtcgcgt catagaagac atcgcgcgcc
     1741 acgcccatcc agtcgcgcag ccgctcgccg ctcgcatcgt cgaacggaat tccggacgca
     1801 tgcacccttc ttccaggcgc ctgggcggcg atcagcacgc gcgcctgcgg atgcgcctgc
     1861 agcaccggcc gcgggccgag cggcaggtgc ccggcgcaat gcctgcacgc gcggacctcg
     1921 gacagcaagg tcgcgaacgc ggtcatgccg atcgcatctc ctcacgactt cgccaccact
     1981 gcgggcatcg gcttctcgtt gacgacgagc tggaacacgt cgggccggct gtagtggccc
     2041 gcggcgtcga agtcgaactt gccgcggccg atctccgcca gatcgagttc ggccgtcaga
     2101 atcgtctcgc cctcgaaatt cggccccgcc agcaccttgc ccagggggct cacgatcgcg
     2161 ctgccgccgc gcatcagcac cgcatcgggc gaatcgccga gcgacacgcg caccgacgcg
     2221 gggaaatcct tcttgcgcag gtactggcac gcggtcagca cgaagcagcg cccttcgagc
     2281 gcgatgtgct gcatcgacgc gagccacgtg tcgcggtcgt ccgcggtcgg cgcgcagtag
     2341 agcgcaacgt ttttcgcgta catcgccatt cgcagcatcg gcatgtagtt ttcccagcag
     2401 atcaccgagc cgatgcgccc gtacgggctg tcgaccacgg tgagcgtcga gccgtcgccg
     2461 aagccccaga tcatccgctc cagcgcggtc ggcatcagct tgcggtgctt gccgagcagc
     2521 gcgccgtcgg gaccgaagaa gagcaccgtg caatagcagg tgcccagttc gcgttcgatc
     2581 acgccgacca ccaggtacac gtggtgcgcg gccgcggctt cgccgagccg ctgcgtgtgc
     2641 ggtccgggca cctcgatcgc ggcatcgaga tacaggcgga actcctcacg cccgaccgga
     2701 tcgcgcgcgc cgaccacgag gccgtagttc aaccctttcg gatagccgac gatgaacgcc
     2761 tcgggaaaca gcacgagctt cgcgccgttg gccgcggcat ccgccgtcag ccgcatcgcc
     2821 ttgtcgacgc acgcgccggt gtcgaacggc accgaaccgg cctgcacgac cgcgacgcga
     2881 accttgctct cccgcgcgac tgaagtcatc gtgatctcct ttggttgcgg gcagacgcta
     2941 cccgttcgaa tcgatcggtt tcagcggcga cgcgggtcgg ctcatccgtg ccgggtgaat
     3001 agaatcggcc ggattgcgga tgcgtcgatg cgcctgcact cgggaaatac cgaggcgtcg
     3061 ccgagacctg ccgcccatac actggtccga tcgcttttgc cccgctcgtc tcctgcgcgg
     3121 gcgcccccgc ccggagacga cttgcatgga acataacatc tcgctgatca cgacggtcgc
     3181 cgtcggtctc ggactggctc tggtgctcgg cttcgcggcc gtgcgggtca ggataccggc
     3241 gctcgtcggg tatctgctcg ccggcatcct catcggaccg gcgacgccgg gcttcgtcgc
     3301 agacgtcggc atcgcttcgc agttgtcgga aatcggcgtg atgctgctga tgttcggtgt
     3361 cggactgcat ttctcgctcg gcgacctgct cgcggtcaag cgcatcgccg tgccgggcgc
     3421 ggtggtgcag atggggatgg cgacggtgct gggcatggcg atcgcctggt ggtggggctg
     3481 gagtttcggg cacggactgc tcttcggcgt gtcgctgtcg tgcgcgagca cggtggtgct
     3541 cctgaaggcg ctggagtccc aggggctgct cgaatcgatg aacgggcgca tcgcggtggg
     3601 ctggctcgtc gtggaggatc tcgcgaccgt gctgatcctc gtgctgctgc cggcgctggc
     3661 gcccgtgctc ggcggaacca acgcgccggc cgcggacagc gtcccgctct ggcgcacgat
     3721 cggactgacg ctgctgcagg tctcggcctt catcgccgtc atgctgatcg ccggtcgtcg
     3781 cgtgctgccg tggctgctgt ggcaggttgc gcagacgggg tcgcgcgaac tgttcacgct
     3841 gctcgtggtc gccgcggcga tcagcatcgc ctacgccgca gcgcaattgt tccacgtgtc
     3901 gtttgcgctc ggcgccttct tcgccggcat ggtgatgcgg gagtccgagt tcagccatcg
     3961 cgctgccgag gaatcgctgc cgctgcgcga tgcgttcgcg gttctcttct tcgtcgccgt
     4021 cggcatgctg ttcgacccca gcgtgctgat cgaggagccg gcgcgcgtgc tgatcacggt
     4081 tgccatcatc gtcgtcggca aatcgatcgc ggcgatggcg ctggtgctcg ccttgcgtta
     4141 tccgctcaac accgcgctga cggtggccgc gagtctggcg cagatcggag agttctcctt
     4201 cattctggca ggcctgggga tgacgctcgg cctgctctcg gccgaggcga tgagcctggt
     4261 actcgccggt gcgctgatct cgattgcgct caaccccttc gtctttgccg ccgtcgcgcc
     4321 gatcagaaac tggatcatcg aacgctcgga gctggcgcgc cggctcgaga gccgtggcga
     4381 cccgtacgcg gagcttccga ccagcacgga acggaagtac ctcgcgggcc aggccgtgct
     4441 ggtgggctac gggcgggtcg gacagcggat cgcggaggcg ttgcaggcca acggcattcc
     4501 gttcgtcgtc gccgaacaga atcgcgaaca ggtcgagtcc ctgcgcgcgc agggcatcgc
     4561 cgccgtctcc ggagacgcca ccgaaccgtc cgtgctgatt caggcgcaca tcgccgacgc
     4621 ggcgatgctg gtcgtcgcgg cgccggatcc ggtcggcgtg cggcgcatcg tcgaaaccgc
     4681 acgcgcgctc aatcccgaca tcgaggtcgt gttgcgcacc cacggcgagg acgaatcgaa
     4741 actgctgcgc aaggaaaaga tcggcacggt gttctacggc gaggaggaac tcgcgcgggg
     4801 catgacgagc catgtactgc agcgcttcgc tccgaaatcg gcttgactcc caaacgcgcg
     4861 gcggcgccgc gcgcgtcccg caaccggacc gctttgcgaa aggaacccga catcatggtt
     4921 cgactgcatc gcctgctcgc ggccaccgat ctctccgcac cggcgcgcca tgccgccgag
     4981 cgggccgctc tgctcgcccg ccatctcgga gcgtccctcg acctcgccca tgtcgccagc
     5041 cttgcgcccc tggaaaagtt gcggcggctc gttgccgacg tcccggctca gctggatgaa
     5101 cgcgtgctcg aggtcgcacg cgaggaaatg ctggaactcg ccgcaacgct gcgccagcgt
     5161 cacggcattg cggccgaagt gcgcgtcgta tcgggccagt tgctcccgcg gctatcccag
     5221 ctggcggacg cgatcgccgc cgatctgatc gtcctcggcg cccgcggcgc cagcttcatg
     5281 caacaccggc tgctcggttc gagcgcggag cgcctggtgc ggcgggcctc ccgacccgcg
     5341 ctggtcgtga agcagaccgc ccatgagccg taccggaacg tgctcgtgcc ggtggacttc
     5401 tcgccgcact ccctgccggc gctgcagcgc gcccgcgcga tcgcacccga tgccgcgatc
     5461 gcgctgctcc atgcatgcga agtaccgttc gaaggcaagc tgcggttcgc aggcgtggcg
     5521 gaagcgacga tcaaccacta ccgcgaatcg gcctgggcgc aggcgctgca atccatgcag
     5581 gcgctgtgcg aggccgcagg gctgccgccg cgcagcgtgc gcgtgctgct gctccacggc
     5641 gacccggtgc agcagattct ccagcaggag caggagcgcg attgcgacct gatcgtgatg
     5701 ggcaagcatg gagaaagcgc gatcgaggat ctgctgcttg gcagcgtcac ccgccatgtg
     5761 ctcgcgcgct cccagtgcga cgtcgtcgta tcggtctgag ccgtttgctg cccatcgacg
     5821 cggtgcgccg acggcaaatg tcgcgcctgg ccactgcatc cgaccggctg ctcatccggc
     5881 acccgcgtag gtgcccgcgc catgcggcgc ggcgcggaac tcgggcagcg cctccgcgcg
     5941 tgcggagaac tccgccagcg ccggatgcgc gtcggcgcgc acgatctccg acaccgaggt
     6001 ctgggtgatg aagtgccacg cgaccgccgt gctgacgccg gcctggtcga tcgtcgcgcc
     6061 cgtgacggcc agcggcgcgc gcgcaacctc ggcctcgagc gccgcgcacg cggcgagcaa
     6121 ctggccgctc acgcgctcga gccacggcgc gtgctgcttt tcggccgggc gcagcgtgcg
     6181 ctcgtagatg atctgcacgc tcttctcgca cgcggcgagc gcgagcccga tcacgcgcag
     6241 cgcgcgcgtg cgcgcggcaa ggccggacgg catcagcgag cggggcgcgg ccagatgctc
     6301 cgcgtaatcg aggatcagcg tcgagtccat cagcaccgtg ccgtcgtcgc acacgaacgt
     6361 gggcgccttg acgaccgggt tgatggcgct gaactcatcg aaggtgcgga acaccgacag
     6421 cgaccggtgc tcgaacggca gtccgagcag ttgcagcgag accgcgacgc gccgcacgta
     6481 tggcgaatcg agcatgccga tcagttgcat cacgaacctc cccgtctggc ctgcagcgcg
     6541 gcgcggcgcg ccaggctctc gctcgaccag acctcggccg cgtcgtaata cgcctcgacc
     6601 gcgcgtgcgc ccggcggcag cacgacccac ggctgcttcg acgcggtgaa gatgtggatg
     6661 tcgggcggca tccggtcggg ttcgtcgagc gtgccgacgc gcacgaagcg caccgcgtcg
     6721 ccgccgcccg cgtagttgct ccacacggcg acacggcagc gcgggcagcg gctgatcttc
     6781 tggcccctgc cgctcgccga cggcgtgtcg atcacctcga ccgtgcctga tagcagttgc
     6841 acgcgatcgg cctcgatcac ggcgttcagc gcgaacgagg cgccggtctc ccgctggcac
     6901 cagcggcaat ggcagcagtg cacgatcagc ggccgcgtcg tcatccggta gcgcacgtag
     6961 cggcaggtgc agccgccgtc gaagggccga tcgtccgcca tggtcaacgc tcaccgtgca
     7021 ttcgtcagcg cgccggctcc gagcgccgtg gcgacgcagg cggactgcgt gcgccggctc
     7081 actggtcgtc ggcgatgatt tcgtgcgtgg tgttgtccac cacgaccgga cagtcgaaat
     7141 aggccagcga cggctcggcg ccgtacttct cgcgcacgaa cgccttccat gcatcggtgt
     7201 acagcgcttc cgcctcgcgg cgcgagcgcc acaggtacac gccgccggcg gtctgtccgt
     7261 cctcggacag cacgtagtac ttgcgcacca gacccggcat gtcccggtac cggggcgccg
     7321 tgctctggaa gatgcggcgc gcttcagcga cgccgagacg ctgcggcaac ccgaaagtcg
     7381 cgatcacggt gatcatcggt cctccctgga gcccgatacg tggcgaaaac agtatcaggc
     7441 cgggccacgg cggtcgcgcg gcccgcctac gctttcgcgc gatgcgcgcc gagcgtggcg
     7501 agcacgacga gcgcgagcgc cgcgatgccg gcgagcgtgc gcacgatgtg ccagtgccac
     7561 catgtatcgc gcagttgcgc ccagtttgcg gccggtgcct gggcgctcca cgtcatcacg
     7621 atcgcattga tcggctggtt ggcgaaccgc gtcaccagtc cggcgacgat catcagcacg
     7681 gcagccgccg ccagcagcgt gcggtcgcgc gcatcgtcgc gtgcagtgac cgcgagcgcg
     7741 atggcgagga cgatgcacac cgctcccatc gccggcagca gcacgttgag cgaacggatc
     7801 gcattctgct gctgctcgac gtaagccgac gccgacagcc cggcgggatc gaatccaacc
     7861 cagacgccga acatcgtgcc gaccagcaac gccagcagca tcagcaggac gaagcggatg
     7921 atgtttgccg tcatgtttgc acctcacccc aagtccgcgc gcagcggcag ttccagcacg
     7981 acttcattgc cgtcgacccg gccggtgtgg cggaagccca agccgagata gaacggctcc
     8041 gggctgccgg ggcccggcac gaacgacagt tcgagtgcag cgaagacgcc tttcgcgcgc
     8101 acatgctcga tcacctgcat cagggcggcg cggccgaccc cgcggccctg gaagcgctcg
     8161 tccaccatga ggcgccacac gccgactttc ggttgctccg gcggcggcgt gcgcagcgac
     8221 tcgtcggcca gcatcacgaa cccggccggc tcttcgtcga cgtagatcgc gcgataccac
     8281 gcctcgtcgg agaacagcgc ctgcgcgagc gataccgcgt tcggcgcgac gaaacccttc
     8341 tggctctcgg ccaccgcgag cttgaccacc gggatcaccg tctcggacgt gatctcgcgc
     8401 agcgtgaccc gggcggaggg tgcagtcatc ggcggaacgc aggtttcggt cgcagtgtcg
     8461 attggtcagg ccgcgcgctc gcgcgtcaga tccgccagct tcagcaccgt cgcccagttg
     8521 cgcgtggtgg tctccgcggg gggcttgccg agcaacgcgg cgccggcctt gctcttcacg
     8581 attccttcgg ggcaccaaag gtaagcggcg tgcgcgccga tctgcaatcg ctcgttttcc
     8641 cggaccagcg cccgcagcgc gccgagctgc gcgagcgaag ccgcgtcgcg cgtgaatgcg
     8701 accagcaggc gcgcggcgtc ggtcgcgacg ccggcgagcg cattctccgc gacggcggcc
     8761 gcgatgtccg cggccggctt cacgagcacc ggcacgtcga tgtcgagcgt gcgcgcgagc
     8821 gccgcgcgaa tgcgttccgc ctgcacgggc ggcgcggcgc tgccaccgtc gaacaccgcg
     8881 ttgccgctgg ctagcaacgt gcgcacgttg cgatggccga gcgactcgag cacgcggcgc
     8941 aggtccgcca tcgcgaggcg gttggctcgc ccgacgttga ctccccgcag cagggcgaca
     9001 tagcgcttca tgcggccgga tcagaagcga tcatgtcggc cacgcgccag ccgacgcctg
     9061 catcgtgttg cgacgggccg accgcgcgca gccgcgatgt gggggtcgat gtccgtacga
     9121 atctcccgta cgtcgaatcc gaagcgtctc cgcttgacgc cgctcgcgcg cggcgacggc
     9181 cgcttctctc gtcacgcgcg ctcctttgcg cgcagcaccc ccgcgcggcg cagcaggtcg
     9241 agcacgagcc ccgcgaacgc cgccagccac gccgccagcg cgacgtacag gaacacggac
     9301 ggcaggaagc ggaggaagtc gaagctcatc gcctcgatca tctcgtgcgt gcttgccgcg
     9361 tacatgccga gcgggaacac cgcgccccag tacagcgggt cgtagcgcag cgggaagcgc
     9421 cggtagacgt gccgccacag cgcgaggatc agcagcatcg ggatccacca cgttccggtg
     9481 gcccagtaga agaccgtgaa gcccttgatg aacggcagca gcgacagcag gaatggcgcg
     9541 tccggcgagt tgatgatcag cagcgacccg gccagcgtcg agatcgccat cgcgcccatg
     9601 ttgatccagt acgggggcga caggtccccc ggcgcgagcg tgaagaacgt gtagcggtag
     9661 aagatcagcg acatcatcca gatgtacagc atgccgcccc acagccacat cgacagcgcg
     9721 aggaagttga tttccagccg gccgggctgg ccgaagtgcg gcgcgagcag cgcgctcagc
     9781 acggcgatcg actgcgtggc caccaccgcc agcagccagg cgccgctgat gccttcggcg
     9841 agcgccggct tgtgcgcctt gatcgtcagc cccgcgatga tcgtgtaggt gaatccgatc
     9901 cagagcacga tcgccacgcc ccacagcgcg acgcccgcgc ggtcgcccgc cgcgagcacg
     9961 acgaactggc tgccgagcac gctggtgccg gccaccgtcg tgaagaagcc cggaccgcgc
    10021 aggtggtcga ccatgtcggt aaagaaatgc cgcgggtagc gtgccgcgcg cagcagcgtc
    10081 agcagccaca gcaccgcgta ggccgcgatg ttgagcgcga acagcacctg cgccagacgc
    10141 ggcaggtcga gcatgtgggc ggccagcgac acgattccgg tcgccatcac catgccgaaa
    10201 tacgccgggg acaagccgtc gagaccgccg agtccgcgcg cgttcgcgcc cgcgccggtg
    10261 gcctgcgacg cgtgtccgcc gtgcgtcgtc atgcacgccg ctcgaacgcg agcttcaggc
    10321 ccaggccgac gagaacgacg ccgctggtgc gatacagcca ggcaagcgca tccggtcgcg
    10381 agcgcaacca tcccgacaga aaccccgcgc cgaagccgac cgggcccttg accagaaacg
    10441 tcaggccggc gaaggccagg ccgagcgcaa agaccgacag cgtcgggtgc gccgcgtgcg
    10501 gcagcacgaa ctgcggcagg aaggcgaaat agaagactgc gatcttcgga ttggatatgt
    10561 tcgaaaacgc gccgtcgaag aacagccgcc gcagcgtccg cggcgtgccg gccgccacgg
    10621 cgaattcatg ccgcccggac cgaagcagtt gcacgccgag gtaaacgagg taggccgcgc
    10681 cggcgaactt gagcgcgacg aagagccatt cggaggtgcg caggatcgcg ccgagcccaa
    10741 gcgtggcgag aatcgtatgg ccgacgagcc cgacactgac gcctgccgcg gtggccacac
    10801 ccgctgcgga accctgcgcg atcgagcgcg acatgaccag catcatgtcc tgccccggcg
    10861 tgacgatcag aacgatcgac gcgaagatga acagcagcca ggtcacttcg agcatggatc
    10921 tcctccggcc tgcggcgcac gcgggcgcaa cgtccggcaa tcgtactcag ggccggtccg
    10981 acggtgccgc cgaaccccgc aaggcgcgtt caactcatcg cgcgggaaga atgagctgag
    11041 tctgtgtgac gacggcacac agcttgcccg attccgccct gactatggtc tgccacacca
    11101 tcgtcgtgcg acccttgtga aacggcgtgg actcgccgac gacggccgtc ccgaccggcg
    11161 cgccgccgat gaacttggtg gacgactcga ccgtcgtcgt gcgcgcgccg gccggcaagt
    11221 tcagaaacgt gccgaccgcg ccgagcgtgt cggcgaaggc catgacggcg ccgccgtgca
    11281 gtgtgtttcc ggtcgtgcac agatccggcc tgacctgcat cgtgccgacg acgcgatcgg
    11341 cggtggtttc cttcagcgcg atgtccagca gccccggcaa cagcggcgcc agcagcgcct
    11401 ggagcgattc aacgccgtgc atgtcatcct ccctgcaagt cgagcacgcg atcgaggccg
    11461 cggtgcacgc cgaccaggcc ggtcaccttg cggatcgcga tcagcgcgcc gtcgacatag
    11521 ggccgcgcgc tggcgccggc gcgatggcgc agcgtgagcg tctggtccgg catgccgaag
    11581 tgcacgtcga cgccgagtac gtgtcccggc agccgcaccg cgtgcacttg cgttccggcg
    11641 acgttggcac cgcgcgtctc gcgcggaccg atcgcgtcgt ccggcccgat cgtgacctgc
    11701 ggcgg
//
LOCUS       DBNHBY010000314         3553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1439224, whole genome shotgun sequence.
ACCESSION   DBNHBY010000314 DBNHBY010000000
VERSION     DBNHBY010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1439224"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC16HB_07685"
     CDS             <1..659
                     /locus_tag="AC16HB_07685"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5606710.1"
                     /translation="VSVEGELGCLGSLETMRGDKEDGHGAEITMTKEMLLTDPDQAAD
                     FVKRTQLDALAIAIGTSHGAYKFTRKPTGDILAIERIKQIHKRIPNTHLVMHGSSSVP
                     QDLLAEIRKFGGQMKETYGVPVEEIQEAIKHGVRKVNIDTDIRLAMTAAIRKFLAENP
                     SAFDPRDYLKPARAAAKAICKQRYQQFGCEGRASQIQPVALDKVAHKYRTGELSQHVH
                     "
     gene            complement(727..1866)
                     /locus_tag="AC16HB_07690"
     CDS             complement(727..1866)
                     /locus_tag="AC16HB_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5606711.1"
                     /translation="MEEAYLGSARRRQFLGRGSATVLGSVFGGLLAGIEPARAQALTG
                     PVPEVDRLAVRVVTDAYHHAFEPTRKVGDIEVQRFAFALSKDLPNKTLQNEWGLSLHC
                     ESARGSEKRQVLIDFGYTGGTLNNNLELLKIDPAQLDALVLSHGHYDHFGGLVGFLQA
                     AKGKLKGGLPFYLGGEECFCTRELGVGDSAGNFGYLDRKAIADAGLKVLLADQPSLIA
                     GHAFTTGRITRASFERVLSPTRMKVGRQDGIGCAPDGLPEAKRTMTVVPDDFDHEQAT
                     CFNVKGKGLVVMTSCGHRGVVNSVRGAMKVSGVNKVHAILGGFHLAPHPDEYLRETVA
                     ALKEFKPDFLIPMHCTGEAFIAIALREMPLQTVRSSTGTRYLFGA"
     gene            2034..>3553
                     /locus_tag="AC16HB_07695"
     CDS             2034..>3553
                     /locus_tag="AC16HB_07695"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR5606712.1"
                     /translation="MQAPAKWIYAFSEGDASKKMLLGGKGANLCEMTRIGLPVPPGFT
                     VTTEACLAYLERGQFPPGLMEEVRAHLARVEASTGRRFGGRTNPLLVSVRSGSALSMP
                     GMMDTILNLGLNADSLAGLIAATGDARFGWDAYRRLIQLFGKVALGVPDEAFDRAMAA
                     LKLEVSVRTDIDLTAEHLQELVQRDLDIVRVHTGQPFPDDPYRQLELAIAAVFRSWNG
                     KRAIDYRRQFRITPQMANGTACNICTMVFGNLGNDSATGVGFTRNPATGEGIVYGEYL
                     VNAQGEDVVAGIRTPKPIAAMAEEMPEQHRQLMQLAATLEKHYREVQDFEFTIERGKL
                     WALQTRNGKMNAQALVRTSVEMAREGLIDRRRALLRVAPTMLEQLLVPRLDPSFKGSP
                     LATGLPASPGAAAGMIVFDADTAETRGRAGEKIILVREETKPEDIHGFFAAQGILTSR
                     GGKTSHAAVVARGMGKPCVSGAESIVVDVHNKVARVGPAVLKEGQVITIDGSTGAV"
BASE COUNT          634 a         1194 c         1177 g          548 t
ORIGIN      
        1 gcgtgtcggt cgagggcgaa ctgggctgcc tcggttcgct cgagacgatg cgcggcgaca
       61 aggaagacgg ccacggcgcc gagatcacga tgacgaagga gatgctgctg accgatcccg
      121 accaggcagc agacttcgtc aagcgtacgc agctcgacgc gctggcgatc gcgatcggca
      181 cctcgcacgg cgcgtacaag ttcacgcgca agccgaccgg cgacatcctg gcgatcgagc
      241 ggatcaagca gatccacaag cgcatcccga acacgcatct ggtgatgcac ggctcgagca
      301 gcgtgccgca ggacctgctg gccgagatcc gcaagttcgg cggccagatg aaggaaacct
      361 acggcgtgcc ggtcgaggag atccaggaag cgatcaagca cggtgtgcgc aaggtcaaca
      421 tcgacaccga cattcgcctc gcgatgaccg cggcgatccg caagttcctg gccgagaatc
      481 cgtccgcctt cgacccgcgc gactacctta agcccgcgcg cgcggccgcg aaagcgatct
      541 gtaagcagcg ctaccagcag ttcggctgcg agggccgcgc cagccagatc cagccggttg
      601 cgctcgacaa ggtcgcgcac aagtaccgca cgggggaact gtcccagcac gtgcactgag
      661 cgcgcgggcg ggaaaacaag acggcgccgc cggcgccgtc tcttgtcttg cagacgcacg
      721 cgatcgctac gcgccgaaga ggtagcgcgt tcccgtcgac gagcgcacgg tctgcagcgg
      781 catctcccgc agcgcgatcg cgatgaacgc ttcgccggtg caatgcatcg ggatgaggaa
      841 atcgggcttg aattccttca gcgcggcaac cgtttcgcgc agatactcat ccggatgcgg
      901 cgcgagatgg aagccgccga ggatcgcgtg cactttgttg acgccggaga ccttcatcgc
      961 gccgcgcacc gaattgacca cgccgcgatg accgcacgag gtcatcacga ccagcccctt
     1021 gcccttgacg ttgaagcagg tcgcctgctc gtggtcgaag tcgtccggca ccacggtcat
     1081 cgtgcgcttg gcctcgggaa gcccgtccgg cgcgcagccg atgccgtcct ggcgtcccac
     1141 cttcatgcgc gtcggcgaca gcacgcgctc gaacgacgcg cgcgtgatgc gtccggtcgt
     1201 gaacgcgtgt ccggcgatca gcgagggctg atcggcgagc agcaccttca gtcccgcgtc
     1261 ggcgatcgcc ttgcggtcga ggtagccgaa gttgccggcc gaatctccca caccgagctc
     1321 gcgcgtgcag aagcattcct cgccgccgag ataaaaaggc aggccgccct tgagcttgcc
     1381 cttcgccgct tgcagaaagc cgaccaggcc gccgaagtga tcgtagtggc catggctcag
     1441 caccagcgca tcgagctgcg ccgggtcgat cttcagcagc tcgaggttgt tgttcagcgt
     1501 gccgccggtg tagccgaagt cgatcagcac ctgccgcttc tcgctgccgc gcgccgactc
     1561 gcagtgcagc gacagccccc attcgttctg cagcgtcttg ttcggcaggt ccttcgacag
     1621 cgcgaaggca aaccgctgca cctcgatgtc gccgaccttg cgcgtcggtt cgaacgcatg
     1681 gtgatacgca tcggtcacca cccgcaccgc gagccggtcc acctccggca ccgggcccgt
     1741 gagcgcctgc gcgcgcgccg gctcgatccc cgccagcaat ccgccgaaca ccgagcccag
     1801 aaccgtcgcg ctgccacggc cgagaaactg ccggcgacgg gcactgccca gataggcctc
     1861 ttccatcttc tcctctcccc gtggttggtg actgcggggg cgatccttgc acggccgccg
     1921 cgagggttca atcacctacc ggcgccgtga ccgcgccgcc ggagtgctgc cgccgatcaa
     1981 ttcggtcggt gcgatccggt gcaatcctgc acgtgaatgc aagggaggat ctgatgcagg
     2041 ccccagcgaa gtggatctac gccttcagcg aaggcgacgc cagcaagaag atgctgctcg
     2101 gcggcaaggg agcgaatctg tgcgagatga cgcggatcgg actgccggta ccacccggtt
     2161 tcacggtgac gaccgaagcg tgtctcgcgt atctcgagcg ggggcagttt ccgccggggt
     2221 tgatggaaga ggtgcgcgcg cacctggcgc gcgtcgaagc gtccaccggc cgccgcttcg
     2281 gcggccgcac gaatccgctg ctggtgtcag tgcgctccgg ctcggcgctg tcgatgccgg
     2341 gcatgatgga cacgatcctg aatctgggtc tgaacgccga ctcgctcgcg ggcctgatcg
     2401 ctgcgaccgg cgacgcgcgc ttcggctggg acgcgtaccg gcgcctgatc cagctcttcg
     2461 gcaaggtcgc gctcggcgtg cccgacgagg cgttcgatcg cgcaatggcc gcgctgaagc
     2521 tcgaggtcag tgtgcgcact gacatcgatc tcaccgcgga gcacctgcag gaactcgtgc
     2581 agcgcgatct cgacatcgtg cgcgtccaca ccggccagcc gtttccggac gatccgtacc
     2641 ggcagctcga actcgcgatc gccgccgtgt tccgctcctg gaacggcaag cgcgcgatcg
     2701 actatcgccg gcaattcagg atcactccgc agatggccaa cggcacggcg tgcaatatct
     2761 gcacgatggt gttcggcaac ctcggcaacg attcggccac cggggtcggc ttcacgcgca
     2821 accccgcgac gggtgaaggc atcgtctatg gcgagtacct cgtcaacgcg cagggcgagg
     2881 acgtcgtcgc cggcatccga acacccaagc cgatcgccgc catggcggag gagatgcccg
     2941 agcagcaccg gcaactgatg caactcgctg caacgctgga gaagcattac cgcgaggtgc
     3001 aggacttcga gttcacgatc gagcgcggca agctgtgggc gctgcagacg cgcaatggca
     3061 agatgaacgc gcaggcgctg gtgcgcacct cggtcgagat ggcgcgcgaa gggctgatcg
     3121 accgccggcg cgcgctgctg cgcgtggcgc ctacgatgct cgagcaactg ctggtgccgc
     3181 ggctcgatcc gtcgttcaaa ggcagcccgc tcgcgacggg actgcctgcg tcacccggtg
     3241 cggccgccgg catgatcgtg ttcgacgccg acaccgcaga aacgcgtggc cgcgcgggcg
     3301 agaagatcat cctggtgcgc gaggaaacca agcccgagga catccacgga tttttcgccg
     3361 cgcagggcat tctcacctcg cgcggcggca agacctcgca tgcagcggtg gtcgcgcgcg
     3421 gcatgggcaa gccgtgcgtg tcgggcgcgg aaagcatcgt cgtcgacgtg cataacaagg
     3481 ttgcgcgcgt cggcccggcg gtgctcaagg aaggtcaggt gatcacgatc gacggctcga
     3541 ccggcgcggt gta
//
LOCUS       DBNHBY010000315         5434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_966284, whole genome shotgun sequence.
ACCESSION   DBNHBY010000315 DBNHBY010000000
VERSION     DBNHBY010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_966284"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1621
                     /locus_tag="AC16HB_07700"
     CDS             158..1621
                     /locus_tag="AC16HB_07700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5606713.1"
                     /translation="MPFDLETLPQRFRRLAPAADHCALRVVEETSETTSVRQDVPEPT
                     SKFVDRGAMVTLIDGDGIGYAATSDLSDAGLRAAIARAAEWARLSRGRSVFAGEPPRL
                     PAARGRYASRTTQPVTWNKADRIDLLMRESRACRIDDRIVDWEASLWTIRTRSLQLTA
                     DGGEALQEIDMTIPSLHVVANQGHITQVRSWGGRGNGFCQQGGLEVIERSGFVGGGRA
                     TAEEALQLLAAPNCPSGKLDVILMPDQMMLQIHESIGHPLELDRILGDERNYAGTSFV
                     TLDMFGTYPYGSSLLNVTFDPTLEIEFASFAFDDEGLRAQREYLIRDGILVRPLGGTV
                     SQQRAQRIRPDIGGVATARASAWNRPPIDRMSNLNVEPGSSSLAEMVAAVDDGILMKT
                     NTSWSIDDSRNKFQFGCEWGQRIRNGKLAEVVRNPNYRGVSATFWRSLAMVGDASTYE
                     ILGTPYCGKGEPNQTIRVGHASPACRFTDVDVFGAEE"
     gene            1618..2940
                     /locus_tag="AC16HB_07705"
     CDS             1618..2940
                     /locus_tag="AC16HB_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR5606714.1"
                     /translation="MKELFFALADHAIAQLAGREVLLAEFSGEASDFVRVNRGRVRQP
                     LSVRQAYLTLTLIEGRRHDGITLALTGSGNEDRAAVTHAIESMRTDLPTLPEDPYLLY
                     ATDVQSSDHERGGKLPAPEQAVDDVVGLATGLDVVGLLASGPVYRGFANSLGQRNWHA
                     VDAFLFDWSVYHAADKAVKCNYAGAEWDRAELSRRIDAAREQLAHLARPVKTIAPGDY
                     RAYLTSAALDELLWMLNWGGVSEKEQRTRQSALQRLVDGEAQLSQQVTLREHTAQGLA
                     PAFDDAGFARPPAVDLIRAGRHAGSLVSPRTAQEYGIQPNGADGSESMHSMELTAGVL
                     PQSDALAALDTGIYVGNFHYLNYSDRPSARVTGLTRFATFWVEHGRIAAPLSVMRFDD
                     TLYRMLGARLEGLTRESEWIMNASTYGCRSVETSRVPGALLAGLTLTL"
     gene            3449..4864
                     /locus_tag="AC16HB_07710"
     CDS             3449..4864
                     /locus_tag="AC16HB_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HIR5606715.1"
                     /translation="MKTRLLVAALGAALGCATSAALAADPVNGKLIYTTGKNGTMACS
                     ACHGSDPNQNQMNIKNGSNWTVIKSAIGSVSLMSQYASVLTDTDLQDIAAFITDPTSA
                     TPTPKAALAPTSINFGNQTINIASNAQTASFTNSGTAPLTLQSITLSGANASDYTRGG
                     TCAANAVLNPGTGCTIDVTFTPLAVGTRNASVSITHNAGGSPTALALTGAGVSSVTLA
                     PAITFTPTTLAFGSQVLGATTTAKTVQLANTGTAALSLTGLQLAGTNPGDFAQTSDCP
                     LPGALAVGASCTISVTFTPTSAGARGATLTAASNATGTPQVALSGTGTTPTTPTNALT
                     LAPMSIDFGSRRVGMTSAPRKVQVVNTGTVNVTISAISVTGDYVQVNNCTGAALAPAG
                     SCAVTLRFKPTAVGARAGDLAITSTATGSPHHVALTGNGLAPRTKGSAAAPELECETE
                     DDASGCSATVSPLGTMPSKGR"
     gene            complement(4892..>5434)
                     /locus_tag="AC16HB_07715"
     CDS             complement(4892..>5434)
                     /locus_tag="AC16HB_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR5606716.1"
                     /translation="VTSEQRRYAKVINFGLIYGMSAFGLASNLGIERDAARNFVDRYF
                     TRYPGVKQYMDRTRELAKRQGYVETVFGRRLWLPEINSPNGPRRGGAERAAINAPMQG
                     TAADLIKLAMIAVHDWLVRENLRTKLVMQVHDELVFEVPESELDMVRERVPALMSGVV
                     QLKVPLLAEVGVGSNWDEAH"
BASE COUNT          935 a         1910 c         1787 g          802 t
ORIGIN      
        1 cgtacgtggc gcaggaacgg cgccggagtg tagaggtgga atagacagcg cccgccgccg
       61 gtagaatcca tgtatcccaa gttcatgcgc cgcgcctcca caagaagcgc cggcgctact
      121 gttgcccgcc gcgactcggg cgggtgcgaa ggaaaccatg ccattcgatc tcgaaacgct
      181 gccgcaacgc ttccgccgtc tcgcgccagc cgccgatcac tgcgcgctgc gcgtggtcga
      241 ggaaacctcc gaaacgacct cggtgcgaca ggacgtaccg gaacccacgt cgaaattcgt
      301 cgaccgcggc gcgatggtca cgctgatcga tggcgacggg atcggctacg cggcgaccag
      361 cgatctgtcg gacgccggtc tgcgcgcggc gatcgcgcgc gccgccgagt gggcgcggct
      421 atcgcggggc cgcagcgtgt tcgcaggcga gccgccccgg ctgccggccg cgcgcggccg
      481 ctatgcgtca cggacgacgc agcccgtcac gtggaacaaa gccgaccgca tcgacctgct
      541 gatgcgcgag tcgcgcgcgt gcagaatcga cgaccgcatc gtcgactggg aagcgagcct
      601 gtggacgatc cgcacgcgtt cgctgcaact gacggccgac ggcggcgagg cgctgcagga
      661 gatcgacatg acgatcccga gcctgcacgt ggttgccaac caggggcaca tcacgcaagt
      721 gcgttcgtgg ggcgggcgcg gcaacgggtt ctgccagcag ggcggactgg aagtcatcga
      781 gcgctcgggg ttcgtcggcg gcggtcgcgc gacggccgag gaagcgctgc aactgctcgc
      841 ggcgcccaac tgcccgagcg gcaagctcga cgtgatcctg atgccggacc agatgatgct
      901 gcagatccac gagtcgatcg ggcatccgct ggaactcgac cgcattctcg gcgacgagcg
      961 caactacgcc ggcacgagct tcgtcacgct cgacatgttc ggcacgtacc cgtatggctc
     1021 gagcctgctg aacgtcacgt tcgatccgac gctcgagatc gagttcgcga gcttcgcctt
     1081 tgacgacgaa ggcctgcgcg cacagcgcga gtacctgatc cgcgacggca tcctggtgcg
     1141 tcctctcggc ggcacggtgt cacagcagcg cgcgcagcgg atccgccccg acatcggcgg
     1201 cgtggccacg gcacgcgcca gcgcgtggaa ccggccaccg atcgaccgca tgagcaacct
     1261 gaacgtcgag ccgggcagtt cgtcgctggc ggagatggtc gcagcagtcg acgacggcat
     1321 cctgatgaag accaacacgt cgtggtcgat cgacgactcg cgcaacaagt tccagttcgg
     1381 ctgcgaatgg ggccagcgca tccgcaacgg caagctggcc gaagtcgtgc gcaatcccaa
     1441 ctaccgcggc gtctcggcga ctttctggcg ttcgctggcg atggtcggcg acgcctcgac
     1501 gtacgagatc ctcggcacgc cctactgcgg caagggcgag ccgaaccaga cgatccgcgt
     1561 cggccatgcc agcccggcgt gcaggttcac ggacgtcgac gtgttcggag cagaagaatg
     1621 aaagaactgt tcttcgcact cgccgatcat gcgatcgcgc agctcgccgg ccgggaagtg
     1681 ctgctggctg aattctccgg cgaggcatcg gatttcgtgc gcgtcaaccg cggccgtgtc
     1741 cggcagccgc tgtcggttcg gcaggcgtac ctgacgctga ccctcatcga aggccgccgc
     1801 cacgacggca tcacgctcgc gctgaccggc agcggcaacg aggatcgcgc ggcggtcacc
     1861 cacgcgatcg agtcgatgcg caccgacctg ccgacgctgc cggaggatcc gtacctgctg
     1921 tacgcgaccg acgtgcagtc gtcggaccat gagcgcggcg gcaagctgcc cgcgccagag
     1981 caggcggtcg acgacgtcgt gggccttgcc accgggctcg acgtggtcgg gctgctcgcc
     2041 agcgggccgg tgtatcgagg tttcgccaac tcgctcgggc agcgcaactg gcacgcggtc
     2101 gacgcgttcc tgttcgactg gtccgtctat cacgccgccg acaaggcggt gaagtgcaac
     2161 tacgcgggcg ccgaatggga tcgcgcggaa ctgtcgcgcc gcatcgacgc cgcgcgcgag
     2221 caactcgcgc acctggcgcg cccggtcaag acgatcgcgc ccggcgacta ccgcgcgtac
     2281 ctgacgtccg ccgcgctcga cgagctgctg tggatgctca actggggtgg cgtctcggaa
     2341 aaggaacaac gcacccggca gagcgcgctg cagcggctgg tcgacggcga ggcgcagttg
     2401 tcgcagcagg tcaccctgcg cgagcacacc gcgcagggac tcgcgcccgc gttcgacgat
     2461 gcgggctttg cgcggccgcc cgcggtcgat ttgatccgcg ccggccgcca cgcaggtagc
     2521 ctggtgtcgc cacgaaccgc gcaggagtat gggatccagc ccaatggcgc cgacggcagc
     2581 gaatcgatgc actcgatgga actcaccgcg ggcgtactgc cgcagtccga cgcgctggcc
     2641 gcgctcgaca ccggcatcta cgtcggcaac ttccattatc tgaactactc ggaccggccg
     2701 tccgcgcgcg tcacggggct cacgcgcttc gcgaccttct gggtcgagca cgggcggatt
     2761 gccgcgccgc tgtcggtgat gcgcttcgac gacacgctgt accggatgct cggcgcgcgg
     2821 ctcgaaggcc tgacgcgcga aagcgagtgg atcatgaatg ccagcaccta tggctgccgc
     2881 tccgtcgaga cgagccgtgt gcccggcgcg ctgctggccg gcttgacgct gacgttgtag
     2941 cgatccggca gggacggagt ctcgccgcgg accctctgcg gcatcgccgc ggcccgccga
     3001 gcgcggttga tcgccgcggc aacgggttga ttcattctcg aagccatgcc gctaacgtcg
     3061 cgccgacatc gtcgcgtcac cggcacggcg cacacgcgca agcggcacgg cgcacgggta
     3121 ccgcgatcga gtgtcgccgc cattgactag tgcgtgcggc atttttgggc cggccgtgca
     3181 aaccgcgctc gcgcgtgctc gctcattcga atggaccggc gcgcaggcac acacttgccg
     3241 cgcgatgcgc gccacgacga gaggttcagt ggccgcgcca taccgcccgc gacatcccgt
     3301 gcgccgccac ggatcgccgc gcttcatccg ccggcttaat catcagccgc gagtgacacg
     3361 cgcgctgcac gcacgaagac tccgcgccgc atcaggcacc aacactgttc tcttctgcaa
     3421 cccgcctgat cgacgaaggg agagtttgat gaagacacgc ttattggtcg cggcgctggg
     3481 ggccgcgctg gggtgcgcca cttccgcggc gctcgctgcc gatccagtca acggcaagct
     3541 gatctacacg accggcaaga acggcacgat ggcctgcagc gcatgccacg gttcggaccc
     3601 gaatcagaat cagatgaaca tcaagaacgg ttccaactgg acggtgatca agtccgcgat
     3661 tggttccgtg tccttgatga gccagtacgc cagcgtgctg accgatacgg atctgcagga
     3721 catcgctgcc ttcatcaccg atccgaccag cgccacgccg acaccgaagg ccgcgctggc
     3781 gccgacttcg atcaacttcg gcaaccagac gatcaacatc gcgtcgaacg cgcagacggc
     3841 gagcttcacc aacagcggca ccgcgccgct gacgctgcag tcgatcacgc tgagcggcgc
     3901 caacgcgtcc gactacacgc gcggcggcac ctgcgcggcg aacgccgtgc tcaaccccgg
     3961 caccggctgc acgatcgacg tcaccttcac gccgctggcg gtcggaacgc gcaacgcaag
     4021 cgtcagcatc acgcacaacg ccggcggcag cccgaccgca ctcgcattga cgggcgccgg
     4081 cgtttcgtcc gtcacgctgg cacccgcgat cacgttcaca ccgaccacgc tggcgttcgg
     4141 ctcacaggtg ctcggcgcca cgacgacggc gaagaccgtg caactcgcga ataccggcac
     4201 cgcagcgttg tcgctgaccg gccttcagct cgccggcacg aatccgggcg actttgcgca
     4261 gacgtccgac tgcccgctgc cgggcgcact cgccgtcggc gcaagctgca cgatcagcgt
     4321 caccttcacg ccaacgtccg ccggcgcccg cggcgcgacg ctgacggcag catccaacgc
     4381 gaccggaacc ccgcaagtcg cgctgtccgg caccggcacc acgccgacca ccccgaccaa
     4441 tgccctcacg cttgcaccga tgtcgatcga cttcggatcc aggcgcgtgg gaatgacgtc
     4501 ggcgccgcgc aaggtgcaag tcgtgaacac cggtaccgtg aacgtgacga tcagcgcgat
     4561 cagcgtgacc ggagactacg tgcaggtgaa caactgcacg ggcgctgcgc tggctccggc
     4621 ggggtcatgc gccgtaacgc tgaggttcaa gcccacggcg gtcggcgcgc gcgccggcga
     4681 cctcgcaatc acgagcacgg cgaccggcag tccccaccac gtcgccctga ccggcaacgg
     4741 tcttgcgccg cggacgaagg gctccgccgc cgcacccgag ctggagtgcg agaccgaaga
     4801 tgacgcatcg ggctgcagcg cgacggtttc gccgctcggc acgatgccgt cgaaaggtcg
     4861 ctgacgacgt cgcgggacgg ggcgcgctgg ctcaatgcgc ctcgtcccag ttgctcccga
     4921 ctcccacctc cgccagcaac ggcaccttca gctgcaccac cccgctcatc agcgccggca
     4981 cgcgttcgcg caccatgtcg agctccgact cgggcacttc gaacaccagt tcgtcgtgca
     5041 cctgcatcac gagcttggtg cgcaggttct cgcgcaccag ccagtcgtgc acggcgatca
     5101 tcgcgagctt gatcaggtcc gccgcggtgc cctgcatcgg cgcattgatg gccgcacgct
     5161 cggcgccgcc gcggcgcggt ccgttgggtg agttgatctc gggcagccac aagcggcgac
     5221 cgaacacggt ttccacgtac ccctgccgct tcgccagctc gcgcgtgcgg tccatgtact
     5281 gcttgacgcc tggataacgg gtgaagtaac ggtcgacgaa gttgcgcgcc gcgtcgcgct
     5341 cgatgccgag gttcgatgcc aggccgaacg cgctcatgcc gtagatcagg ccgaagttga
     5401 tcaccttggc gtaacggcgc tgctcgctcg tgac
//
LOCUS       DBNHBY010000316         3498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_830370, whole genome shotgun sequence.
ACCESSION   DBNHBY010000316 DBNHBY010000000
VERSION     DBNHBY010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_830370"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="arsS"
                     /locus_tag="AC16HB_07720"
     CDS             complement(<1..749)
                     /gene="arsS"
                     /locus_tag="AC16HB_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR5606717.1"
                     /translation="MHATLPLLRKTDFPAIRRRAVETLQVNLGYTCNQSCVHCHVNAG
                     PNRTEQMDRATIDLVLDVVTAKDIKTLDLTGGAPELNPHFRDLVREARARGVHVIDRC
                     NLTILFEPGQESLAEFLARHEVEIVDSLPCYLEDNVDRQRGKGVFEKSVQALKLLNAL
                     GYGSGLPLSLVYNPQGPSLPPNQVRLEADYRRMLGEHFGIVFTRLLVLANMPIARFGS
                     TLISKGQFRDYMNTLRQAHRTENVEHVMCRSL"
     gene            complement(1063..1635)
                     /locus_tag="AC16HB_07725"
     CDS             complement(1063..1635)
                     /locus_tag="AC16HB_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5606718.1"
                     /translation="MDASAIQQVLGELRPTLVRIARLQLRNDTWAEDVVSETLLAALE
                     SAASFAQKSQIKTWVVGILKHKIIDQFRRVNREISSDAQMEAAEADDLEDLYDPSGHK
                     ANPPLDWGDPHANLERTQFFEILQMCVDKLPAGLGRIFMMREWLEYETDQICKEMAIT
                     STNCFVMLHRARMRLRECLEMNWFAAGKAR"
     gene            complement(1645..2550)
                     /locus_tag="AC16HB_07730"
     CDS             complement(1645..2550)
                     /locus_tag="AC16HB_07730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5606719.1"
                     /translation="MSNRTYDPITPHRVAFLGLGVMGFPMAGHLARAGHHVTIYNRTT
                     KKSERWVEEYGGRAAPTPREAATGAQIVFACVGNDDDLRSVVLGEHGAFAGMARDAIF
                     VDHTTASAAVARELYGAARSRGLHFVDAPVSGGQAGAVNGQLTVMCGGDAAPFGHAQA
                     VAMAFARAVTLVGASGSGQLCKMVNQIAIAGMVQALSEAIVFGQKSALDMKLVLQVIG
                     KGAAQSWQMDNRGTTMIDDKFDFGFAVDWMRKDLGLCLDEARRNGAPLPVAALVDQFY
                     ADLQAMGGGRWDTSSLIRRLKHTSH"
     gene            2646..3371
                     /locus_tag="AC16HB_07735"
     CDS             2646..3371
                     /locus_tag="AC16HB_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR5606720.1"
                     /translation="MALSLFLAAAAAAACTVTSPAHTVALVELYTSQGCSSCPPADRW
                     LSKLQPSDRVVPLALHVGYWDYIGWKDPFARREFNERQRWLASLNGNRTVYTPGVFVQ
                     GRETPRWYDARGFDAAVAAINAQPAHARIELAAKFDGGQIEARASVSAADARQPQVLL
                     ALVQSGNVTRVGAGENRGETLANDHVVTQWSGPLASGAPMVQRWPAPDVSSDRLRVVA
                     VVQDAVRGQPLRAVALPLAACGK"
BASE COUNT          532 a         1201 c         1183 g          582 t
ORIGIN      
        1 agcgagcggc acatcacgtg ctcgacgttt tccgtccggt gcgcctgccg cagcgtgttc
       61 atgtagtcgc ggaactgtcc cttgctgatc agcgtggagc cgaagcgcgc gatcggcatg
      121 ttggccagca ccagcaggcg cgtgaagacg atgccgaagt gctcgccgag catccggcgg
      181 taatcggcct cgagccggac ctggttcggc ggcagcgacg gcccctgcgg gttgtaaacg
      241 agcgacagcg gcagcccgct gccgtagccg agcgcattga gtagcttgag cgcctgcacg
      301 ctcttttcga acacgccctt gccgcgctgg cggtcgacgt tgtcctcgag gtagcagggc
      361 agcgagtcga cgatctcgac ttcgtgccgc gcgaggaact ccgccagact ctcctgcccg
      421 ggctcgaaaa ggatcgtcag gttgcagcgg tcgatcacgt gcacgccgcg cgcgcgcgct
      481 tcccgcacga gatcccggaa gtgcgggttc agttccggtg cgccgccggt caggtcgagc
      541 gtcttgatgt ccttcgcggt gaccacgtcg agcacgaggt cgatggtggc gcgatccatc
      601 tgttcggtgc ggttcggtcc cgcgttcaca tggcagtgca cgcaggactg gttgcaggtg
      661 tagccgagat tgacctgcag cgtttcgacc gcgcggcggc ggatcgctgg aaagtcggtc
      721 tttctgagca gcggcagggt ggcgtgcacg gcgtctcccg attgattcga tcttcgtcgg
      781 ggcagccgcg caaaccttac ccgtccgtgc ctgcgccgac gacccgccga ttggttacgg
      841 atacgagcgg aattcccggc cggattgggt gttacttgtc gaggcgccga accgcttcgc
      901 gcatgaaacc catctgccga tcgacgcgcg cgcagaaggc gcagaagcgc aggtgcagcc
      961 agagcctgaa gcgttcgacc aggctcatcg gggcgtcatc catccgcgcg ctgagcagct
     1021 cgtgcgcatg ctggcacttg atcctgagcc aggcgggcgg cctcaccttg ctttccccgc
     1081 ggcaaaccag ttcatttcca ggcactcccg cagccgcatc cgggcgcggt gcagcatcac
     1141 gaagcagttg gtcgaggtga tcgccatttc cttacagatc tgatcggttt cgtattcgag
     1201 ccactcgcgc atcatgaaga tccgaccgag cccggccggc agcttgtcga cgcacatctg
     1261 caggatctcg aagaattgcg tgcgctccag attcgcgtgc gggtcgcccc agtccagcgg
     1321 cgggttggcc ttgtggccgc tggggtcgta caggtcctcg aggtcgtcgg cctccgcggc
     1381 ttccatttgc gcgtcggacg atatctcgcg gttgacacgg cgaaactggt cgatgatctt
     1441 gtgcttgagg atcccgacca cccaggtctt gatctgcgac ttctgcgcga agctggcggc
     1501 gctttcgagc gcggcgagca gcgtttccga gaccacgtcc tcggcccagg tgtcgttgcg
     1561 caactgcagg cgcgcgatgc gcaccagggt cggccgcagc tcgccgagta cctgctgaat
     1621 cgcgctcgca tccatgttcg agtgtcagtg gctggtgtgc ttcaaccgcc ggatcagcga
     1681 cgacgtgtcc cagcggccgc cgcccatcgc ctgcaggtcg gcatagaact ggtcgaccag
     1741 cgcagcgacc ggcagcgggg cgccgttgcg acgggcttcg tccaggcaca ggcccagatc
     1801 cttacgcatc cagtccaccg cgaagccgaa gtcgaacttg tcgtcgatca tcgtcgtgcc
     1861 gcggttgtcc atctgccagc tctgcgccgc gcccttgccg atcacctgca gcaccagctt
     1921 catgtcgagc gcggacttct ggccgaacac gatggcctcg gacaatgcct gcaccatgcc
     1981 ggcgatcgcg atctgattga ccatcttgca caactgcccg ctgccgctgg cacccacgag
     2041 cgtcaccgcg cgcgcgaacg ccatcgccac cgcctgcgcg tgcccgaacg gtgcggcgtc
     2101 gccgccgcac atcaccgtca gctggccgtt gaccgcgccc gcctgcccgc ccgacaccgg
     2161 cgcgtcgacg aagtgcaggc cgcggctgcg cgctgcaccg tacagctcgc gcgccacggc
     2221 ggccgatgcg gtggtgtggt cgacgaagat cgcatcgcgc gccatgccgg cgaacgcgcc
     2281 gtgctcgccc agcaccacgc tgcgcaggtc gtcgtcgttg ccgacgcacg caaacacgat
     2341 ctgcgcgccg gtcgccgcct cgcgcggcgt gggcgcggcg cggccgccat actcctcgac
     2401 ccagcgctcg gatttcttcg tcgtgcggtt gtagatggtc acgtgatggc cggcgcgcgc
     2461 gaggtggccc gccatcggga accccatcac gccgagcccg aggaatgcaa cccggtgcgg
     2521 cgtgatcggg tcataggtcc ggttcgacat cgttcgccct gcggaaaaag acgcgcggac
     2581 aatacaccgt ctgtaagagc gtcggcggcg ccgcgaccaa acacgcggcc ccgaaggaga
     2641 acccgatggc cttatcgctg ttcctcgccg ccgccgcagc ggcggcctgc accgtgacca
     2701 gccccgcgca tacggttgcg ctggtcgagc tgtacacgtc gcagggctgc tcgagctgcc
     2761 cgccggcgga ccgctggctg tcgaagctgc agccgtcgga ccgcgtcgtc ccgcttgcgc
     2821 tgcatgtcgg ctactgggac tacatcggct ggaaggatcc gttcgcgcgg cgcgaattca
     2881 acgagcgcca gcgctggcta gcgagcctga acggcaaccg caccgtctac acgccgggcg
     2941 tgttcgtgca gggacgcgag acgccgcgct ggtacgacgc gcgcggcttc gacgccgcgg
     3001 tggccgcgat caacgcgcag ccggcgcatg cccgcatcga actggcggcg aagttcgacg
     3061 gcggccagat cgaggcacgc gccagcgtca gcgccgccga cgcccggcag ccgcaggtgc
     3121 tgctggcgct tgtgcagagc ggcaacgtca cccgagtcgg cgccggcgag aaccgcggcg
     3181 agacgctggc caacgatcat gtcgtcacgc aatggagcgg gccgctggcg tccggcgccc
     3241 cgatggtgca gcgctggccc gcgccggacg tgtctagcga ccggctgcgc gtcgtggccg
     3301 tcgtgcagga tgccgtgcgc ggtcagccgc tgcgggcggt cgcgctgccg ctggcggcct
     3361 gcggcaagta gggactgcga gtgcgaggcg gcgcctgacg cgcagccgca ccttgcgttg
     3421 gttcagcgcc gcaccacgcg cgtcggcagc gtttggggcc caggcttgcg cgccagctcg
     3481 cgcatgaact gcggcagc
//
LOCUS       DBNHBY010000317         4602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1372532, whole genome shotgun sequence.
ACCESSION   DBNHBY010000317 DBNHBY010000000
VERSION     DBNHBY010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1372532"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC16HB_07740"
     CDS             complement(<1..368)
                     /locus_tag="AC16HB_07740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5606721.1"
                     /translation="MIFVTGGAGFIGANFVHDWIGCEAEPLLVVDKLTYAGNLDNLAE
                     LRGDPRYGFVQADICDRATVAALLGQHRPRALLHFAAESHVDRSIDGPGEFVRTNVNG
                     TFELLEAVRSWLSTCDEASRA"
     gene            complement(563..1600)
                     /locus_tag="AC16HB_07745"
     CDS             complement(563..1600)
                     /locus_tag="AC16HB_07745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5606722.1"
                     /translation="MGNIENPGRTGQRLPTHVDLGTLYRRPLFDLGGMGHEELRGIGT
                     PIYDEGSVQRFLHSQFREHAQTYATNYTDNAYFTWLISNALKRIQWTEEAQHGRTILD
                     IGSGAGNSIFPLLQLCPESTLIASDLSVELLALLKHSLVAKGLWDRCALLQLNAEELD
                     FEPESFDLVVGAAILHHLFAPERTILGCGRILKPGGHAIFFEPFENGSAILNLIYQRL
                     MREPGRPALEEKVASFLQLLANDNVVRRGRDKSQPIFAQIDDKWLFTKSFFEEHARLA
                     GFSECEIYPANPPAGQFSRQIEVNLRLGLNRDRTALPPWAWKIIDEYDNAFSIDLMKD
                     LLPEGVIILRK"
     gene            complement(1728..>4602)
                     /locus_tag="AC16HB_07750"
     CDS             complement(1728..>4602)
                     /locus_tag="AC16HB_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5606723.1"
                     /translation="KQVIVRTPWFGDHGAVVLLTGEDPYFELPIDRTALARCHTGAVL
                     EVDMGWPMSADYALFVGELSALQASLAQARADAQRSAQLRESLERELADRRAEVQARA
                     SEKVRARQLEQKLDAETASVQRLQLESAQLAAERERFRTQADQLGITVQQVQRDIADL
                     RRRVEQQRAQGNAVVRERDALRARLAQIQDSRLYGLAALFSPSLRSGIELRGMAPRSA
                     LAGVSRLRELPPSAAVVDIVVPVYKGLAETQTCLESVLRSAPDRPFRVVVVDDASPEP
                     EVSAYLRQLRDRDARVVLIENPTNAGFVTSVNRGMQRDSHRDVVLLNSDTEVAGDWLD
                     RLQRAAYSDPHVGTATPFSNSATICSYPRFCEDNRLPDGTTTAELDAMFAAENAGRTV
                     DIPTAVGFCMYVRRDCLNAVGLFDVENFGRGYGEENDFCMRAHKAGWRHVLALDTFVL
                     HAGGVSFGAEKARRVEDAQDTLKRLHPEYEQMVQRHLAQDPARSARLAVDVARIRHSA
                     LRGVLFVSHFGGGGTERHVRELAALMKDQANSFLLRPTDSGETLLEWLQPGEGFCLAF
                     RLDDELEALVAALHALNVCHVHYHHLLGQSPCIWGLPALLGVTHDFTVHDFYSACPQI
                     TLTDRASRYCGEQGPAQCAECLRRTPAPGRVSIDTWRDGYRPLLETARHVIAPSGDAA
                     ERIRRYFPAANVVHVPHFDVEDAPPVVKPAPLAEERPLRIVVIGALSTIKGADVLESA
                     AIEAARRGVRLEFHLLGFAYRPLQTQPKAFLTVHGPYQEDDLPGLLAWLKPDLAWFPA
                     LWPETYSYTLSACLTARLPIVAPDLGAFAERLHGRPWTWVCPWDQTASTWVDFFEAIR
                     REHFRTGLEPRAAAGRDFAASGFSYQHDYLPKKSLAQEPARLAEDFLRHYRPGRTISR
                     NGRGAKRVMLGLAVRAKRAPIVGGLARRVPLRWQARARSWLKG"
BASE COUNT          812 a         1565 c         1431 g          794 t
ORIGIN      
        1 gcacgcgacg cttcatcgca tgtgctcagc cacgaccgaa cggcttcgag cagctcgaac
       61 gtgccgttga cgttggttcg aacgaactcg ccaggcccgt cgatggagcg atcgacgtgg
      121 ctttccgccg caaagtgcag cagcgcgcgc ggccggtgct gccccagaag cgccgccacg
      181 gtggctcggt cgcaaatgtc ggcctggacg aagccgtagc gcggatcccc gcgcagttcg
      241 gccaggttgt cgaggtttcc cgcgtaagtc agcttgtcga cgaccagcag gggctccgct
      301 tcgcacccga tccagtcatg gacaaagttc gcaccgatga atccggcgcc tccggtcaca
      361 aagatcatgt cagacggccg cagcccatgc ggccgcgaaa agatttgaat tcgaatgtca
      421 agcttaacgc agcccgatcc gtgacgagtt cgggttcctg acgaggttcc tgcacgggaa
      481 cgaggcctca accgtcacac gccacgcagg cgtcaacccg gctggatctc tcgtccttgc
      541 gcctggcgag cgccggcacg cgctatttcc tgagaatgat gactccttcg ggcagcagat
      601 ccttcatcag gtcaatcgag aaggcgttgt cgtactcatc gatgatcttc catgcccagg
      661 gcggcagcgc cgtccgatcc cgattgaggc caaggcggag attgacttcg atctgccgac
      721 tgaactgtcc ggccggtggg ttcgccggat agatctcgca ttcggagaag cccgcgagcc
      781 gcgcatgttc ttcgaagaag gacttggtga agagccattt gtcgtcgatc tgggcaaaga
      841 tgggctgcga cttgtcgcgt cctctccgca cgacgttgtc gttcgccagc agttgcagaa
      901 acgacgcgac cttctcttcc agcgcgggcc gtcccggctc ccgcataagg cgctgataga
      961 tgaggttcag gatggcactt ccgttctcga acggctcgaa gaagatcgca tggccgccgg
     1021 gcttcaggat tcgaccacac ccaagaatcg ttcgctcagg tgcaaacaga tggtgaagga
     1081 tcgcagcacc cacgaccaga tcgaatgact ccggttcgaa gtcgagttcc tcggcgttca
     1141 attgcagcaa ggcacagcgg tcccaaagtc ccttcgcgac cagactgtgc ttcagcagcg
     1201 ccagcaactc gacgctcaga tcgcttgcga tcaacgtgga ttccggacaa agctgcagca
     1261 aagggaagat cgaattgcct gcgcccgatc cgatatcgag gatcgttcgt ccgtgctgcg
     1321 cttcctcggt ccactggatt cgtttcagcg cgttcgagat gagccaggtg aagtaggcgt
     1381 tgtcggtgta attcgtggcg taggtctgtg catgctcgcg aaactgcgaa tgaagaaatc
     1441 gctggacgct gccctcgtca tagatgggag tgccaatgcc ccgcagttct tcgtgcccca
     1501 ttccacccag gtcgaacagc ggccgtcgat acagcgtccc caggtcgaca tgcgtcggca
     1561 gtcgctggcc ggtacgcccc gggttctcga tattccccac tcgtcagtct ccttgccgcg
     1621 tcacgacggt tcgctcccgt tgactctcaa aaggtcgccg aagacgcgca cgggctgccc
     1681 agccgcgagc agacgggacg ttcggcgtgc gtgcgcggga atcgacttca gcccttcagc
     1741 caagacctgg cacgcgcttg ccagcgcaat ggaacgcggc gcgccagacc accgacgatg
     1801 ggcgcgcgct tggcgcggac agccagtccg agcatcacgc gcttcgcgcc ccgcccattg
     1861 cgggaaatcg ttcggcccgg acggtaatgc cgcaggaaat cttcggcgag ccgcgccggc
     1921 tcctgcgcca gcgacttctt cggcaggtag tcatgctgat agctgaaccc ggatgccgcg
     1981 aaatcccgcc ctgctgccgc acgcggctcc agaccggtcc ggaagtgctc gcgtcgaatc
     2041 gcttcgaaaa agtcgaccca cgtcgacgcg gtctgatccc acggacagac ccaggtccac
     2101 ggccgcccgt gcaggcgctc cgcgaaggcg cccagatcgg gggcaacgat cggcagccgc
     2161 gccgtcaggc aagcgctcaa ggtgtagctg tacgtctcgg gccagagcgc cgggaaccac
     2221 gcgaggtccg gcttcaacca ggcaagcagg cccggcagat cgtcttcctg ataagggcca
     2281 tgcacggtca ggaacgcctt cggctgcgtc tgcagcggcc ggtaggcaaa ccccagcaga
     2341 tgaaactcga gccgcacacc gcggcgcgcc gcttcgatcg ccgcgctttc gaggacgtcc
     2401 gctcccttga tcgtgctcaa ggcgccgatc acgacgatcc gcagcggtcg ttcctccgcc
     2461 aacggcgccg gcttcaccac cggcggtgca tcctcgacgt cgaagtgcgg cacgtgaacg
     2521 acgttcgccg ccgggaagta cctgcggatc cgctcggccg catcgccgct gggagcgatt
     2581 acatggcgtg ccgtttccag cagcggccga tagccgtcac gccaggtgtc gatcgacacg
     2641 cgcccgggcg ccggtgttcg ccgcaggcat tcggcgcact gcgcgggccc ttgctcgccg
     2701 caataccggc tggcgcgatc cgtcagcgtt atctggggac aagccgaata gaagtcgtgc
     2761 acggtgaagt catgtgtcac gccgagcaga gccggcagcc cccagatgca cggcgactgg
     2821 cccagcaggt ggtggtagtg aacatggcac acattcaacg cgtgcaacgc ggccaccagc
     2881 gcctccagtt cgtcgtcgag gcgaaacgcg aggcagaagc cttcgcccgg ttgcagccat
     2941 tcgagcaatg tctctccgct atcggtcggg cgcagcagga aggaattggc ctgatccttc
     3001 atcagtgcgg ccagttcccg cacgtggcgt tccgtgccgc cgccgccgaa gtgactgacg
     3061 aacagcacgc cccgcaacgc gctgtgccgt atgcgggcca cgtcgaccgc caggcgcgcc
     3121 gaccgcgccg ggtcctgggc cagatgcctt tgaaccatct gctcgtattc gggatgaagc
     3181 cgcttcagcg tgtcctgggc gtcttccacg cggcgtgcct tctccgcgcc gaagctgacg
     3241 ccgcccgcat gcagcacaaa ggtgtcgagc gcgagcacgt gccgccagcc ggccttgtgt
     3301 gcgcgcatgc agaagtcgtt ttcttcgccg tagccacggc cgaagttctc gacgtcgaac
     3361 aaccccaccg cattcaggca gtcgcgccgg acatacatgc agaagcccac cgcggtcgga
     3421 atgtcgaccg ttcgccctgc attctccgcg gcgaacatcg cgtcgagctc cgccgtcgtc
     3481 gtgccgtccg gcaggcgatt gtcttcgcag aacctgggat agctgcagat cgtcgcgctg
     3541 ttggaaaacg gcgtcgcagt gcccacgtgc ggatcgctgt aggccgctcg ttgcaggcgg
     3601 tcgagccagt cgcctgcgac ttccgtatcg ctgttgagca ggacgacatc acggtggctg
     3661 tcgcgctgca tgccgcgatt gacgctggtc acgaagccgg cgttggtcgg attctcgatc
     3721 agcaccacgc gcgcgtcgcg gtcgcgcaac tggcgcaagt acgccgacac ctccggctcc
     3781 gggctcgcgt cgtcgaccac gacgacccgg aacggtctgt caggcgcgct tctcagcacc
     3841 gattccaggc acgtctgtgt ttcggccagg cccttgtaaa ccggcaccac gatgtcgacg
     3901 actgcggcgg atggcggcag ctcgcgcaac cgggacacgc cggccaacgc gctgcgcggc
     3961 gccatgccgc gcagctcgat cccgctgcgc aacgacggac tgaaaagtgc cgctaggccg
     4021 tacagccgcg aatcctggat ctgcgccagc cgtgcccgca gggcgtcgcg ctcccggacg
     4081 acggcgttgc cctgcgcgcg ctgctgctcg acccgccgcc tcaggtcggc gatgtcgcgc
     4141 tgcacctgct gcaccgtgat gccgagctga tccgcctggg tccgaaacct ctcgcgctca
     4201 gcggccagtt gcgcggattc cagctgcaag cgctgcaccg atgccgtttc ggcgtcgagc
     4261 ttctgctcga gttgccgcgc gcgtaccttc tcgctcgccc gcgcctgcac ctctgcgcgc
     4321 cggtcggcga gttcccgctc gagagactcc cgcagctggg ccgagcgctg cgcgtcagcg
     4381 cgtgcctgcg ccaggctcgc ctgcagcgca ctgagttcac cgacgaacag ggcatagtca
     4441 gccgacatcg gccagcccat gtcgacctcg agcaccgcgc cggtgtgaca acgcgccaat
     4501 gccgtgcgat cgatcggcaa ttcgaaatac ggatcctctc ccgtcaatag cacgaccgcg
     4561 ccgtggtccc cgaaccacgg cgtccggacg atcacctgct ta
//
LOCUS       DBNHBY010000318         4621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_695359, whole genome shotgun sequence.
ACCESSION   DBNHBY010000318 DBNHBY010000000
VERSION     DBNHBY010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_695359"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC16HB_07755"
     CDS             complement(<1..599)
                     /locus_tag="AC16HB_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606724.1"
                     /translation="MSEVVLRVAGLKVAYGGIQAVKGVDLEVRAGELVTLIGSNGAGK
                     TTTLKAITGTQAWSGNIEYMGHSAHGMVPYQLLTRGRAMVPEGRGVFARMTVTENLQM
                     GAFARNDVAEIKADMERMFETFPRLRERARQLAGTLSGGEQQMLAMARALMSRPKLLL
                     MDEPSMGLSPLMVEKIFEVVRSVSAQKIPVLLVEQNARLA"
     gene            complement(744..1538)
                     /locus_tag="AC16HB_07760"
     CDS             complement(744..1538)
                     /locus_tag="AC16HB_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606725.1"
                     /translation="MSDTILKVLGVNKRFGGLQALSEVGIHITRGQIYGLIGPNGAGK
                     TTFFNVITGLYQPDSGTFELDGKPYSPSEPHRVAEAGIARTFQNIRLFPEMTALENVM
                     VGRHVRTKATFFGAVLRTRGERIEEAAIKKRGLELLDYVGIRRYADFQARTLAYGDQR
                     RLEIARALATDPKLLALDEPAAGMNATEKIALRGLLSNIRKDGATILLIEHDVKLVMG
                     LCDRVTVLDYGKVIAEGTPADVQRNPAVIEAYLGAAHKPAAGQEAA"
     gene            complement(1552..2649)
                     /locus_tag="AC16HB_07765"
     CDS             complement(1552..2649)
                     /locus_tag="AC16HB_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5606726.1"
                     /translation="MAAYAATFVVAAVLVALPFAAGYFGNAWVRALAFAALYVMLALG
                     LNIVVGFAGLLDLGYIAFYAVGAYMYALLASPHLTSNFETLAAMFPNGLHNSVWLAIP
                     LGAAIAAFFGVVLGAPVLKLRGDYLAIVTLGFGEIIRIFLNNLNAPLNFTNGPQGITL
                     IDPVTIFGVNFSKTQTPFGLSLPSVYMYYYLFLLLTVVAIVICIRLQDSRIGRAWMAI
                     REDDIAAKAMGVNVRNIKLLAFALGATFGGVSGAMFAAFQGFVSPESFSLMESIIVLS
                     MVVLGGLGHIPGVILGAVLLSMFPELLRHTVVPVQEQLFGKVLVDAEVLRMLLYGLAM
                     VIVMLYRPKGLWPAPEHGVNPAARPGKPQTA"
     gene            complement(2695..3624)
                     /locus_tag="AC16HB_07770"
     CDS             complement(2695..3624)
                     /locus_tag="AC16HB_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606727.1"
                     /translation="MDTLIQQILNGLVLGSLYSLIALGYTMVYGILNLINFAHGEVLM
                     VGAMTALSTILFLRAHFPDAPGWLLLIAGTAVAIPVCVIVSLIIERVAYRPLRNAPRL
                     APLITAIGVSITLQTLAMMIWGRNYMTLPQLLPSDPINFLGATITVTKILVIVLSAVI
                     MLGLMLLVNRTKLGRAMRATAENPRVAGLMGVNPNYVIALTFAIGAALTAVAGVMIAA
                     TYSVVHFYMGFIPGLKAFTAAVLGGIGNIPGAMVGGILLGLIEALGAGYIGQLTGGIL
                     GSNYQDVFAFIVLCLVLILRPSGLLGERVADRA"
     gene            complement(3755..>4621)
                     /locus_tag="AC16HB_07775"
     CDS             complement(3755..>4621)
                     /locus_tag="AC16HB_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5606728.1"
                     /translation="AQKIVDSGAVAVVGHLNSGTSIPASKIYSDVNMTQISPSATNPK
                     LTEQGFKTTFRVVANDNQQGAVLANYAAAEMKAKTIAIVDDRTAYGQGLADVVEKVAK
                     EKGLKVVAREFATDKTTDFNAILTKIRATKPDVVMYGGMDATAGPMAKQMKQLGIKSH
                     LLAGDGVCSPEFIKLAGNAADILTCSMAGEAVEKLAKGEEFKQKYKAKFGTDVQIYSP
                     YSYDAVYVIADAIKRAGKADRSSITAAMPATNYAGLTGQIQFDEKGDIKNGAISMFKV
                     KDGKLEYISTSR"
BASE COUNT          802 a         1573 c         1481 g          765 t
ORIGIN      
        1 gcgagccgcg cgttctgctc gaccagcagc acggggatct tctgcgccga gacgctgcgc
       61 acgacctcga agatcttctc caccatcagc ggcgacaggc ccatcgacgg ctcgtccatc
      121 agcagcagtt tcggccggct catcagcgca cgcgccatcg cgagcatctg ctgctcccca
      181 cccgacagcg tgcccgccag ttggcgcgcg cgctcgcgca ggcgcgggaa cgtttcgaac
      241 atgcgttcca tgtcggcctt gatctcggcc acgtcattgc gcgcgaacgc gcccatctgc
      301 aggttctcgg tcaccgtcat gcgtgcgaac acgccgcgcc cctccggcac catcgcccgc
      361 ccccgcgtca gcagctggta gggcaccatg ccgtgcgcgg aatgccccat gtactcgatg
      421 ttgccgctcc acgcctgcgt gcccgtgatc gccttcagcg tggtcgtctt gccggcgccg
      481 ttggagccga tcagcgtcac cagttctccg gcccgcactt cgagatcgac acccttgaca
      541 gcctggatac cgccgtatgc aaccttgagc ccggcaacgc gaaggacgac ctcactcatg
      601 cccggatctc cattgcgcgc tccctccccg tgaggggagg gacggggagg ggcgggccga
      661 acctgcgccc gcgcgggcgg cgcgcgcagc gcgcagaccg ccgtacgcga ccttcaggcc
      721 ggacaccttg aggaccactt ccgtcatgcg gcctcctgcc ccgccgccgg cttgtgcgcg
      781 gcgccgagat aggcctcgat cacggccgga ttgcgctgga cgtcggccgg cgtgccctcg
      841 gcgatgacct tgccgtagtc gagcacggtc acgcggtcgc acaggcccat caccagcttc
      901 acgtcgtgtt cgatcagcag gatcgtcgcg ccgtccttgc ggatgttcga cagcaggccg
      961 cgcagcgcga tcttttcggt cgcgttcatg ccggcggcgg gttcgtcgag cgcgagcagc
     1021 ttcggatcgg tcgcaagcgc gcgcgcgatc tccagccgac gctggtcgcc gtaggcgagc
     1081 gtgcgcgcct ggaaatcggc gtacctgcgg atgccgacgt agtcgagcag ttcgagtccg
     1141 cgcttcttga tcgccgcctc ctcgatgcgc tcgccgcgcg tgcgcagcac cgcgccgaag
     1201 aacgtcgctt tggtgcgcac gtggcggccg accatcacgt tttccaacgc ggtcatctcg
     1261 ggaaagagcc ggatgttctg gaacgtgcgc gcgatccccg cctcggccac ccgatgcggc
     1321 tcgctcggcg aataaggctt gccgtcgagc tcgaacgtgc ccgagtcggg ctggtacagc
     1381 ccggtgatca cattgaagaa ggtcgtcttg ccggcgccgt tgggaccgat caggccgtag
     1441 atctgcccgc gcgtgatgtg gatgccgacc tcggacagcg cctgcagtcc gccgaaacgc
     1501 ttgttgacgc ccagtacctt gaggatcgtg tcgctcatcg cgcggtgccg ctcaggccgt
     1561 ctgcggcttg cccggccgtg cggccgggtt cacgccgtgc tcgggtgcgg gccacagccc
     1621 cttgggccgg tacagcatga cgatcaccat cgccaggccg tacagcagca tgcgcagtac
     1681 ctcggcgtcg accagcactt ttccgaacag ctgctcctgc accggcacca cggtgtgccg
     1741 cagcagctcg ggaaacatcg acagcagcac cgcgccgagg atgacgcccg ggatgtggcc
     1801 gagcccgccg agaacgacca tcgacagcac gatgatcgac tccatcagcg agaacgactc
     1861 gggcgacacg aagccctgga acgccgcgaa catcgcgccc gacacgccgc cgaacgtggc
     1921 gccgagcgcg aacgcgagca gcttgatgtt gcgcacgttg acgcccatcg ccttggcggc
     1981 gatatcgtcc tcgcggatcg ccatccacgc acggccaatc cgcgagtcct gcagccggat
     2041 gcagatcacg atcgcgacga ctgtcagcag caggaacagg tagtagtaca tgtagacgga
     2101 gggcagcgag aggccgaacg gcgtctgcgt cttggagaag ttgacaccga agatcgtgac
     2161 cggatcgatc agcgtgatgc cctgcggccc gttggtgaag ttcagcggcg cgttcaggtt
     2221 gttcaggaag atgcggatga tctcgccgaa gccgagcgtg acgatcgcca gatagtcgcc
     2281 gcgcagcttc agcacgggcg cgccgagcac cacgccgaag aacgcggcga tcgccgcgcc
     2341 gagcggaatg gcgagccaca ccgagttgtg caggccgttc ggaaacatcg ccgccagcgt
     2401 ctcgaagttg ctggtgagat gcggcgaggc gagcaaggcg tacatgtagg cgccgaccgc
     2461 gtaaaaggcg atatagccca ggtcgagcag gccggcgaag ccgacgacga tgttcagccc
     2521 cagcgccagc atcacataca gggcggcgaa tgcgagagcg cgtacccacg cattgccgaa
     2581 atagccggcc gcgaacggca gcgcgaccag caccgcggcg acgacaaagg tcgcggcgta
     2641 cgcggccatc ggcttgtcgc gcaggctggg gaagaacaat gtcatcgcag gctcctacgc
     2701 gcggtcggcg acgcgctcgc cgagcagccc cgacggccgc aggatcagca ccaggcacag
     2761 cacgatgaac gcgaacacgt cctggtagtt cgagccgaga atgccgccgg tcaactgccc
     2821 gatgtagccg gcgccgagcg cctcgatcag cccgagcagg atgccgccca ccatcgcgcc
     2881 gggaatgttg ccgatgccgc cgagcaccgc cgcggtgaac gccttcagcc ccggaatgaa
     2941 gcccatgtag aagtgcacca ccgagtaggt ggcggcgatc atcacgccgg ccacggccgt
     3001 gagcgcggct ccgatcgcaa acgtcagcgc gatcacgtag ttcggattca cgcccatcag
     3061 gcccgccacg cgcgggttct cggcggtggc gcgcatcgcg cggccgagct tggtgcggtt
     3121 caccagcagc atcagcccga gcatgatcac cgcagacagc acgatcacga ggatcttggt
     3181 caccgtgatc gtcgcgccca ggaagttgat cgggtccgac ggcagcagtt gcggcagcgt
     3241 catgtagttt cggccccaga tcatcatcgc cagcgtctgc agcgtgatcg atacgccgat
     3301 cgcggtgatc agcggcgcga gacgcggcgc attgcgcagc ggccggtaag cgacgcgctc
     3361 gatgatcagg ctcacgatca cgcagaccgg aatcgcaacc gcggtgccgg cgatcagcag
     3421 cagccagccc ggcgcgtctg gaaagtgcgc gcgcagaaac aggatcgtcg acagcgcggt
     3481 catcgccccg accatcagca cctcgccgtg cgcgaaattg atcaggttca gaatcccgta
     3541 caccatcgtg tagccgagcg cgatcagcga atacaggctg ccgagcacaa ggccgttcag
     3601 aatctgctgg atgagggtat ccatgggcct ccacctcgcg tctggttagg ctgcgatcgg
     3661 cgcgatcgcg catcgtatca agccgcgatg gcgcaagcgc accctctcaa aaaaaacggc
     3721 acccgaaggt gccgttcccg tcgcggttcc gaactcagcg cgaggtcgag atgtactcga
     3781 gcttgccgtc cttgaccttg aacatcgaaa tggcgccgtt cttgatgtcg cctttttcgt
     3841 cgaactggat ctgccccgtc agcccggcgt aattggtcgc cggcatcgcc gcggtgatcg
     3901 acgaccggtc ggccttgccg gcgcgcttga tcgcgtcggc gatcacgtat accgcgtcat
     3961 agctgtacgg cgagtagatc tgcacgtcgg tgccgaactt cgccttgtac ttctgcttga
     4021 actcctcgcc cttggccagc ttctcgaccg cctcgcccgc catcgagcag gtcaggatgt
     4081 cggccgcgtt gccggccagc ttgatgaact cgggcgagca cacgccgtca cccgccagca
     4141 gatgcgactt gatgccgagc tgtttcatct gcttggccat cgggcctgcg gtggcgtcca
     4201 tgccgccgta catcaccaca tcgggcttgg tggcgcggat cttggtgaga atcgcattga
     4261 agtcggtcgt cttgtcggtc gcgaactcgc gcgcgacgac cttcaggccc ttttccttcg
     4321 cgaccttctc gaccacgtcc gcgagaccct ggccgtacgc ggtgcggtcg tcgacgatcg
     4381 cgatcgtctt ggccttcatc tcggcggctg cgtagttcgc cagcaccgcg ccctgctggt
     4441 tgtcgttggc gaccacgcgg aacgtcgtct tgaaaccctg ctcggtcagc ttcgggttgg
     4501 tcgccgacgg cgagatctgc gtcatgttga cgtcggagta gatcttcgac gccgggatcg
     4561 aggtgccgga gttcagatgc ccgaccaccg caaccgcgcc cgagtcgacg atcttctgcg
     4621 c
//
LOCUS       DBNHBY010000319         2538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1845933, whole genome shotgun sequence.
ACCESSION   DBNHBY010000319 DBNHBY010000000
VERSION     DBNHBY010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1845933"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC16HB_07780"
     CDS             <1..644
                     /locus_tag="AC16HB_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5606729.1"
                     /translation="NVNILVLDTEVYSNTGGQASKSTPRGAVAKFAAAGKSTGKKDLG
                     MIAMAYGNVYVAQVAMGANPVQTVRTFQEAASWPGPSLIIAYSHCIAHGIDMATGMSH
                     QRDAVKSGYLTLYHYDPRLGMGGSDQPLKLDSRKPTQPLEQFVMKEGRYAMLAQSDPA
                     RAKQLLKAAQHDADARWQLYEQVAGVHRVVTDTQAPATAEEEPQAAKPEESPS"
     gene            641..1645
                     /locus_tag="AC16HB_07785"
     CDS             641..1645
                     /locus_tag="AC16HB_07785"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR5606730.1"
                     /translation="MSVDLRTRYLGLELKHPIVASASPLTSSIDSLKRLQDAGIAAVV
                     LKSLFEEELEHEEMSAHNLMLYGAELSPEAQGFFPEMQYSTGPDKYLELISGAKKALS
                     VPVIASLNGYTTGGWTSIARKFQEAGADAIELNVYFLAASVDDTSSAVEQRYVDLVHS
                     VTQQVSVPVAVKVAPYFSAMANMAARLARAGAKGLVLFNRFLQPDILLDDLEVAPHLV
                     LSTSDELRLALRWIAILRGRVDASLAATGGAHTRDDVLKLLLAGADCVMLASSLLLKG
                     PTHVGTLVRGVQAWMTEREYDSVAQLKGSLSQQSCPDPDAFERANYMKALRSYTSEFV
                     "
     gene            1664..1909
                     /locus_tag="AC16HB_07790"
     CDS             1664..1909
                     /locus_tag="AC16HB_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR5606731.1"
                     /translation="MALIINDDCTACDACVEPCPNQAITAGSPIYLIDPAKCTECVGA
                     HDEPQCVLVCPADCIVPDPANKESKDELMAKYKAMHG"
     gene            1955..2404
                     /locus_tag="AC16HB_07795"
     CDS             1955..2404
                     /locus_tag="AC16HB_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5606732.1"
                     /translation="MSSATIYRTDFPALKGGDVVGEATHRMLTNFVTDLPVVDDDGRL
                     IGILRLERLLASLLPKGALIGFGMDDLSFVSDTLEHLRKQMHSVADTPVRQLMVQAEQ
                     VVHPDTSPVEIVLLLYRGASNVPVVERDTNKLVGMVSARDVLNALQN"
BASE COUNT          466 a          839 c          847 g          386 t
ORIGIN      
        1 agaacgtcaa catcctcgtg ctcgacaccg aggtctacag caacaccggc gggcaggcat
       61 cgaagtccac gccgcgcggc gcggtcgcca agttcgccgc ggccggcaag tcgaccggca
      121 agaaggacct cggcatgatc gcgatggcgt acggcaacgt gtacgtggcg caggtcgcga
      181 tgggcgcgaa tccggtgcag acggtgcgca cgttccagga agcggcgtcg tggccgggac
      241 cgtcgctgat catcgcgtac agccattgca tcgcgcatgg catcgacatg gccaccggca
      301 tgagccacca gcgcgatgcg gtgaagagcg ggtacctgac gctgtaccac tacgatccgc
      361 gcctcggcat gggcggatcc gaccagccgc tgaagctcga ttcgcgcaag cccacgcaac
      421 cgctcgagca gttcgtgatg aaggagggcc gctatgcgat gctggcgcag tccgacccgg
      481 cgcgcgccaa gcaactgcta aaggccgcgc agcacgacgc cgacgcgcgc tggcagttgt
      541 acgagcaggt ggccggcgtg catcgcgtgg tgaccgacac gcaggcgccg gccactgccg
      601 aagaagaacc gcaggcagcg aaaccggagg agagcccgtc atgagcgtcg atctgcgcac
      661 ccgctacctg ggactggagc tgaagcatcc gatcgtggcg tcggcttcgc cgctgacgag
      721 ttcgatcgac agcctgaagc gcctgcagga tgccggcatc gccgcggtgg tgctgaagtc
      781 gctgttcgag gaggaactcg agcacgagga gatgtccgcg cacaacctga tgctgtacgg
      841 cgcggagctg tcgcccgagg cgcaggggtt ctttcccgag atgcagtaca gcaccggacc
      901 ggacaagtac ctggagctga tctcgggcgc caagaaggcg ctgtcggtgc cggtgatcgc
      961 cagcctgaac ggctacacga ccggcggctg gaccagcatc gcacgcaagt tccaagaggc
     1021 cggcgcggac gcgatcgagc tgaacgtcta tttcctcgcc gccagcgtgg acgacaccag
     1081 cagcgcggtc gagcaacgct acgtcgatct cgtgcactcg gtgacgcagc aggtcagcgt
     1141 gccggtcgcg gtgaaagtcg cgccgtactt ttcggcgatg gccaacatgg ccgcacggct
     1201 cgcccgggcc ggcgcgaagg ggctggtgct gttcaaccgc ttcctgcagc ccgacatcct
     1261 gctcgacgat ctcgaggtcg caccgcacct cgtgctgtcg acctcggacg agctgcggct
     1321 cgcgctgcgc tggatcgcga tcctgcgcgg ccgcgttgac gccagcctgg cggcgaccgg
     1381 cggcgcgcac acccgggacg atgtgctgaa gctcctgctc gccggcgccg actgcgtgat
     1441 gctcgccagc agcctgttgc tgaaagggcc gacgcacgtc ggcacgctgg tgcgcggcgt
     1501 gcaagcgtgg atgaccgagc gggaatacga ttcggtcgcg cagctcaagg gatcgctgtc
     1561 gcagcagtcc tgccccgacc ccgatgcctt cgagcgcgcc aactacatga aggcgctgcg
     1621 ctcgtacacc agcgaattcg tctgagcttc aaggagattc ctcatggcac tgatcatcaa
     1681 cgacgactgc accgcctgcg acgcctgcgt ggagccgtgc ccgaaccagg ccatcacggc
     1741 gggctcgccg atctacctca tcgacccggc caagtgcacc gagtgcgtcg gcgcacatga
     1801 cgagccgcag tgcgtgctcg tatgcccggc cgactgcatc gttcccgacc ccgcgaacaa
     1861 ggagtccaag gacgagctga tggccaagta caaggccatg cacggctgat caggtccatc
     1921 ggtacactcg cgcggttcca atggggggac cgcgatgagt tcagcaacga tctatcgcac
     1981 cgatttcccg gcattgaagg gcggcgacgt cgtcggcgag gcgacgcacc gcatgctgac
     2041 caacttcgtg accgacctgc cggtcgtcga tgacgacggc cgcctgatcg gcatcctgag
     2101 actcgagcgc ctgctggcgt cgctgctgcc gaagggcgcg ctgatcggct tcggcatgga
     2161 cgacctgagc ttcgtctccg acacgctcga gcacctgcgc aagcagatgc atagcgtcgc
     2221 cgacacgccc gtgcggcaac tgatggtgca ggccgaacag gtcgtgcatc cggacacctc
     2281 gccggtcgag atcgtgctgc tgctgtatcg cggcgcgagc aacgttccgg tcgtcgaacg
     2341 cgacacgaac aagctggtcg gcatggtatc ggcacgcgac gtgctcaacg ccctgcaaaa
     2401 ctaggaagac cccggcgcga gcgccgggca cttcggtcgg aggagacaca tgcacggcca
     2461 ggctacccat gcggccaccg cgatcttcgg gctgaatccg ctgtggatcg cggtggcgct
     2521 gttcgtcgcg acctacgt
//
LOCUS       DBNHBY010000320         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_18797, whole genome shotgun sequence.
ACCESSION   DBNHBY010000320 DBNHBY010000000
VERSION     DBNHBY010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_18797"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="AC16HB_07800"
     CDS             <1..1984
                     /locus_tag="AC16HB_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR5606733.1"
                     /translation="ELIPAPDFPTAGIIYGLDGVREGYRTGRGRVVMRARTHFEGVDE
                     KSGDKGARPAIIVDELPYQVDKRVLLERMAEPVNEKKLEGISDIRDESDKSGMRVVIE
                     LKRGELPEVVLNNLYKQTQLQDTFGMNFVALVDGAPRLLNLKQMLEAFLAHRREVVTR
                     RTVFELREARDRGHVLEGLAVALANIDEFIAIIKAAPTPPVAKAELMARTWDSALVRE
                     LVTRAGDDRQAFRPQSLEPQFGLQPDGLYRLSETQAEQILQMRLSRLTGLEQDKIVNE
                     YREVMDLIADLLDILAKPERITSIIRDELTALKQEFGDARRSEIVRTAEDLQTEDLIT
                     PQDMVVTLSHAGYIKSQPLADYRAQKRGGRGKQAAATREDDWIDQLFIAHTHDTILCF
                     SNRGRLYWLKVWEVPQGTRTSRGKPIVNMFPLADNEKITVVLPVKHFDALHYVFMATA
                     NGTVKKTELSEFANPRKAGIIACDLDAGDYLIGAAITDGRHDVMLFSDAGKAVRFDEN
                     DVRPMGRQARGVRGMQLEDGQSVIALLVAEDEQQSVLTATENGFGKRTPIAEYTRQGR
                     GTKGMIAIATTERNGKVVAATLVRSDDEIMLITTGGVLIRTRVAEIREMGRATQGVTL
                     IALDEGTKLAGLQRIVESDVDNGAEAAPENNVDTAP"
     gene            1996..3066
                     /gene="pheA"
                     /locus_tag="AC16HB_07805"
     CDS             1996..3066
                     /gene="pheA"
                     /locus_tag="AC16HB_07805"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR5606734.1"
                     /translation="MDDRLKPLRDAIDAVDRKLVELLNERARLAAEVGRVKQEHNAPV
                     YRPEREAEVLDKVAALSAGPLPTATVQAVYREVIAACRALERPITVAYLGPPGTFSEL
                     ALRRQFGAGVVAAPCGSIDEVMRDVEAGSTDYGIVPVENSTEGSITRTLDLLLATPLA
                     IIAEVSLPVHHNLMTKSGTMAGVLRVCAHAQSLAQCVNWLNQHHATLPRVSVASNAEA
                     AKMAAEDPTAAAIASESAAERYGLQSVAVHIQDDPGNRTRFVVVGRQGTTPTGRDKTS
                     LILSVPNKAGAVYHMLTPLAQHGVSMTRFESRPAKSGAWEYWFYVDIEGHEKDPKTAA
                     ALAELRERCAFYKSLGSYPMAN"
     gene            3081..>3358
                     /locus_tag="AC16HB_07810"
     CDS             3081..>3358
                     /locus_tag="AC16HB_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR5606735.1"
                     /translation="MAAVMKADESVPALPYVRAIAPYQAGKPIEELAREFGLKAEHIV
                     KLASNENPLGMPPSARSAIVRELERLADVGRYPDSNGFALKAALASKYG"
BASE COUNT          629 a         1073 c         1168 g          488 t
ORIGIN      
        1 tgaattgatc ccggcaccgg actttccgac ggcggggatc atctacgggc tcgatggcgt
       61 gcgcgaaggc tatcgcaccg gccgtggccg cgtcgtgatg cgcgcgcgga cgcatttcga
      121 gggggtggac gaaaagtccg gcgacaaggg cgcacgtccc gcgatcatcg tcgacgaact
      181 accgtaccag gtcgacaagc gcgtgttgct cgaacgcatg gcggagccgg tcaacgagaa
      241 gaagctcgag ggcatttccg acatccgtga cgagtccgac aagagcggca tgcgggtggt
      301 gatcgagctg aaacgcggcg agctgcccga ggtggtgctg aacaacctgt acaagcagac
      361 gcagctgcag gacacgttcg gcatgaattt cgtggcgctg gtcgacggcg cgccgcggct
      421 gctgaacctg aagcagatgc tggaggcgtt tctcgcgcac cgacgcgagg tcgtcacgcg
      481 ccgcacggtg ttcgagctgc gtgaggcgcg cgaccgcggc cacgtgctgg aaggccttgc
      541 ggtggcgctg gcgaacatcg acgagttcat cgcgatcatc aaggctgcac ccacgccgcc
      601 ggtggcgaag gccgaactaa tggcgcgcac ctgggactcg gcgctcgtgc gcgaattggt
      661 gacgcgggcc ggcgacgacc gccaggcgtt ccggccgcag tcgctcgagc cgcagttcgg
      721 tctgcagccc gatggcctgt accgcctgtc ggaaacgcag gccgagcaga tcctgcagat
      781 gcggctctcg cgtctgacgg ggctggagca agacaagatc gtcaacgagt accgcgaagt
      841 gatggacctg atcgcggacc tgctcgacat cctggccaag cccgagcgca tcacctccat
      901 cattcgcgac gaactgactg cgctgaagca ggagttcggc gacgcacgcc gctcggagat
      961 cgtgcgcacc gcggaagacc tgcagaccga ggacctgatc acgccgcagg acatggtcgt
     1021 gaccttgtcc cacgccggct acatcaagag ccagccgctc gccgactacc gcgcacagaa
     1081 gcgcggcggg cgcggcaagc aggcggcggc gacgcgcgag gatgactgga tcgaccagct
     1141 attcatcgcg cacacgcacg acacgatcct gtgcttctct aaccgcgggc ggctgtattg
     1201 gctgaaggtg tgggaagtgc cgcagggcac gcgcacctcg cgcggcaagc cgatcgtcaa
     1261 catgtttccg ttggcggaca atgaaaaaat caccgtcgtg ctgccggtca agcacttcga
     1321 tgccctgcat tacgtcttca tggcaaccgc caacggcacc gtgaagaaga ccgaactttc
     1381 ggagttcgcc aatccacgca aggccggcat catcgcctgc gatctggacg cgggcgacta
     1441 cctgatcggc gcggcgatca ccgacggccg ccacgacgtg atgctgttct cggacgccgg
     1501 caaggcagtt cggttcgacg aaaacgatgt gcgaccgatg ggccggcagg cgcgcggcgt
     1561 gcgcggcatg cagctcgagg acggccagtc ggtcatcgcc ctgctggtgg ccgaagacga
     1621 gcagcaaagc gtgctgaccg cgaccgaaaa cggcttcggc aagcgcacgc ctatcgccga
     1681 atacacgcgc caaggtcgag gcaccaaggg tatgatcgcg atcgccacca ccgagcgcaa
     1741 cggcaaggtc gtcgcggcta cgctggtacg gtccgacgac gagatcatgc tgattacgac
     1801 cggcggcgtg ctgatacgca cccgcgtggc cgagatccgc gagatggggc gtgccacgca
     1861 gggcgtgacg ctgatcgcgc tcgacgaggg caccaagctc gccggcctgc agcggatcgt
     1921 cgagagcgac gtcgacaacg gggccgaagc cgcacccgag aacaacgtcg ataccgcacc
     1981 gtaggctgag cgcgcatgga cgaccgcctc aagccgctgc gcgacgcgat cgacgccgtc
     2041 gatcgcaagc tggtcgagct gctgaacgag cgtgcgcggc tggcggccga ggtcggccgc
     2101 gtgaagcagg agcacaacgc gccggtgtac cgccccgagc gcgaggccga ggtgctcgac
     2161 aaggtcgccg cgctgagcgc cggtccgctt cccaccgcga ccgtgcaggc ggtgtatcgc
     2221 gaggtgatcg ccgcctgccg cgcactggag cggccgatca cggtggcgta tctgggcccg
     2281 ccgggcacgt tttccgaact cgcactgcgc aggcaattcg gcgccggcgt ggtcgcggcg
     2341 ccgtgcggtt cgatcgatga ggtgatgcgc gacgtggagg cgggcagcac cgactatggc
     2401 atcgtgccgg tggagaactc caccgagggc tcgatcacgc gcaccctcga tctgctgctc
     2461 gccacgccgc tggcgatcat tgccgaggtg tcgctgccgg tgcatcacaa cctgatgacg
     2521 aagagcggca cgatggcggg cgtgctgcgc gtatgcgcgc acgcgcagtc gctggcgcag
     2581 tgcgtgaact ggctcaacca gcatcacgcc acgctgccac gcgtcagcgt ggcgagcaac
     2641 gcggaagcgg cgaagatggc ggccgaggat ccgacggcgg cagcgatcgc gagcgaaagc
     2701 gcggccgagc gctacggcct gcaatcggtc gcagttcaca ttcaggacga tccgggcaat
     2761 cgcacgcgct tcgtcgtggt cggccgccag ggcacgacac cgaccgggcg tgacaagacc
     2821 tcgttgatcc tgtcggttcc gaacaaggcg ggcgccgtgt atcacatgct cacgcccctg
     2881 gcgcagcacg gcgtgtcgat gacgcgcttc gaatcgcgcc cggccaagag cggcgcgtgg
     2941 gaatactggt tctacgtcga catcgagggt cacgagaagg acccgaagac cgcggcggcg
     3001 ctggccgagt tgcgcgagcg ctgcgcgttc tacaagagtc tcgggtcgta tccgatggcg
     3061 aactaggcga aggagcgccg atggctgcag tgatgaaagc agatgagtcc gtgccggcgc
     3121 tgccttatgt gcgcgcgatc gcgccgtacc aggcgggcaa gccgatcgag gagctggcac
     3181 gcgagttcgg gctgaaggcc gagcacatcg tcaagctcgc ctccaacgag aatccgctcg
     3241 gcatgccgcc gtcggcgcgc agcgcaatcg tgcgcgaact cgagcgtctc gccgacgtgg
     3301 gacgctatcc ggactccaat ggcttcgcgc tgaaggcggc gctggcgtcg aagtacgg
//
LOCUS       DBNHBY010000321         3530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1643225, whole genome shotgun sequence.
ACCESSION   DBNHBY010000321 DBNHBY010000000
VERSION     DBNHBY010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1643225"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16HB_07815"
     CDS             <1..203
                     /locus_tag="AC16HB_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5606736.1"
                     /translation="ADERGSREYNLALGQRRADAVRARLQLLGASSAQIETISFGEER
                     PRAAGHDEQSWAQNRRADIAYR"
     gene            377..2032
                     /locus_tag="AC16HB_07820"
     CDS             377..2032
                     /locus_tag="AC16HB_07820"
                     /inference="COORDINATES: protein motif:HMM:NF017081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606737.1"
                     /translation="MQLSIRSIADGAAPRRIAIGLAFCIVYVLVDAATHLLADTWRAT
                     APWYPQPALAVALIVAGGIEFAPAILIAVLLAGWLTPFAGFTSWGLFVACIGAAASYC
                     AAGWAVARWTRWSALDVGLRDLTIFIAVALVTGILVTAVFGLAGYASGLRVGAGLAQL
                     IRQLAIGNSVGLIVAGPLLLQLAAGAWRPWVATPASRRAAPRDLLLFILGMAAVLVLV
                     FGLTPFDEFRMFYLMFLPIIVMAMRYGLPGAALALPIVQVGLIVALTVFGVRATTAFE
                     FQILMLAMALTTLYLGAASSERERAAERVAQGERALREQREALNEAQRIASTAELAAA
                     VAHDLNQPLSAIGTYARACRLLVERGAAEREELLRVLGQLAAESQRAGGYVRRMREFF
                     RSGAMHSERIDVKRLVQRAHAQLRDRLQRESIAWTEVIPSELPPARGDGVQLGAVLDN
                     LLGNACDALQETGGARAIEVSVARVDNAGRPLLRIRVQDTGPGVPLEVREQLFKPLAT
                     SKPHGMGLGLALSRSIAERLGGGLWFDADAPRTTFCLDLPADE"
     gene            2025..2693
                     /locus_tag="AC16HB_07825"
     CDS             2025..2693
                     /locus_tag="AC16HB_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5606738.1"
                     /translation="MNSRHVSAAARRFVYVVEDDAAVRESLRLLLQLRGYETVACERA
                     EEFLAFEAFERPACVLVDVRLPGMSGLDLQRRLAGAQPTLPVIVMTAHGDAATARAAL
                     RDGAIDFLEKPIDESDLLQAVELALQSDVAQLARDREHEDIMHSMQRLTERELDVFTR
                     ITDGLHNREIAEELGISPRTVEVHRARLMEKLAARRVADLFRLRFDLDRRAPDHLPRT
                     ARAR"
     gene            complement(3133..>3530)
                     /locus_tag="AC16HB_07830"
     CDS             complement(3133..>3530)
                     /locus_tag="AC16HB_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5606739.1"
                     /translation="GDVGTARNALKAGAFDFIEKPIDNDRLVELVGAALAEDARRAAH
                     EAEAQDLAERMKRLTQRERQVLSQVVAGKHNREIAADLGISARTVEVYKARVMDKLQV
                     RRVPDLVRLVLNAEGDAGARALDAGTLPA"
BASE COUNT          458 a         1216 c         1270 g          586 t
ORIGIN      
        1 atgccgatga gcgcggcagc cgcgagtaca acctcgcgct cggccagcgc cgtgccgacg
       61 cggtcagggc gcgcctgcag ttgctcggcg cgtcgagtgc gcagatcgag acgatcagct
      121 tcggcgagga gcggccgcgc gcggccggac acgacgaaca gtcctgggcg cagaaccgac
      181 gcgccgacat cgcctatcgc tgagtgtggg cgctgcggcc agcgcgaagg gcgaggaggc
      241 cggggtgggt cagagcagtc cctgtgttac ggttgacgcg tcagtcttcg gttggcggga
      301 cggccgggac gcatgcggcg cacgggtcgt gaccgccgtg cgcgtaccgg cgtcgccggc
      361 ccgggatcgc gtcgcgatgc agctttccat tcgctcgatc gccgacggcg cggcaccgcg
      421 ccggatcgcg atcggcctcg cgttctgcat cgtgtacgtg ctggtcgatg cggccacgca
      481 cctgctcgcc gacacgtggc gcgcgaccgc gccttggtac ccgcagccgg cgctcgcggt
      541 cgcgctgatc gtggccggcg gcatcgagtt cgcgccggcg atcctgattg ccgtgctgct
      601 ggccggctgg ctcacgccgt tcgcgggctt cacctcgtgg gggctcttcg tcgcctgcat
      661 cggtgccgct gcgagctatt gcgcggccgg ctgggcggtc gcgcgctgga cacgctggtc
      721 ggcgctcgac gtgggactgc gcgacctgac gatcttcatc gcggtggcgc tcgtcacggg
      781 catcttggtg accgcggtct tcgggctggc cggctatgcg tcgggactgc gcgtcggtgc
      841 gggactcgcg caactcatcc ggcaactggc gatcggcaat tcggtcgggt tgatcgtggc
      901 cggaccgttg ctgctgcagc tcgccgcggg cgcctggcgg ccctgggttg ccacgcctgc
      961 gtcgcgccgg gccgcgccgc gcgacctgct gctgttcatc ctgggaatgg cggcggtgct
     1021 cgttctcgtg ttcggactca cgccgttcga cgagttccgc atgttctacc tgatgttcct
     1081 gccgatcatc gtcatggcga tgcgctacgg gctgccgggc gcggcgctgg cgctgccgat
     1141 cgttcaggtc gggctgatcg ttgcgctgac cgtgtttggc gtacgcgcga cgaccgcctt
     1201 cgagttccag atcctgatgc tggcgatggc gctgacgacg ctctatctgg gcgcggcgtc
     1261 gagcgaacgc gagcgcgccg ccgagcgcgt cgcgcagggc gagcgcgcgt tgcgcgagca
     1321 gcgcgaggcg ctgaacgagg cacagcgcat cgcatcgact gccgagctgg ccgcggcagt
     1381 cgcgcacgac ctgaaccagc cgctgtcggc gatcggcacc tacgcgcgcg cctgccgcct
     1441 gctggtggag cgcggcgccg ccgagcgcga ggaactgctg cgcgtactcg ggcagctggc
     1501 ggcggagagc cagcgcgccg gtggttacgt gcggcgcatg cgcgagttct tccgcagcgg
     1561 cgcgatgcac agcgaaagga tcgacgtgaa gcggctcgtg caacgcgcac acgcgcaact
     1621 gcgcgatcgc ctgcagcgcg agtcgattgc gtggaccgag gtcatcccgt ccgaactgcc
     1681 gccggcgcgc ggcgatgggg tgcagctcgg cgcggtgctc gacaacctgc tcggcaacgc
     1741 gtgcgatgcg ctgcaggaga cgggcggcgc gcgcgcgatc gaggtcagcg tcgcgcgcgt
     1801 cgacaacgcc gggaggccgc tgctgcgcat ccgcgtgcag gacaccgggc ccggcgtgcc
     1861 gctcgaggtg cgcgagcagc tgttcaagcc gctcgccacc agcaagccgc acggcatggg
     1921 actcgggctc gcgctgtcgc gttcgattgc cgagcgactc ggcggcgggc tgtggttcga
     1981 cgccgacgcg ccgcgtacca cgttctgcct ggacctgcct gccgatgaat agccgtcatg
     2041 tttccgccgc tgcgcggcgg ttcgtctatg tcgtcgagga cgacgccgcc gtgcgcgagt
     2101 cgctgaggct gctgctgcag ctgcgcggct acgagaccgt cgcgtgcgag cgcgccgagg
     2161 agttcctcgc gttcgaggcg ttcgagcgcc cggcgtgcgt gctggtcgac gtgcgcctgc
     2221 ccggcatgtc cgggctcgat ctgcagcgcc gcctggcggg cgcgcagccg acgctgccgg
     2281 tgatcgttat gaccgcgcac ggcgacgctg ccacggcgcg cgccgcgctg cgcgacggcg
     2341 cgatcgattt cctcgagaag ccgatcgacg aatccgacct gctgcaggcc gtcgagcttg
     2401 ccctgcagag cgacgtggcg cagctggcac gcgaccgcga acacgaggac atcatgcaca
     2461 gcatgcagcg gctgaccgag cgcgaactcg acgtcttcac gcgcatcacc gacggcctgc
     2521 acaaccgcga gatcgcggag gaactcggca tcagcccgcg cacggtcgag gtccatcgcg
     2581 cgcggctgat ggagaagctc gccgcgcgcc gggtggcgga tctgttccgg ctgcgcttcg
     2641 atctcgaccg ccgcgcgccc gatcatctgc cgcgcacggc gcgtgctagg taaatcttcg
     2701 taggttgagc gctgatgcgg ccgtgtgaac tgcgtggcac gatgccgtcg gcgtccccgg
     2761 cgatccggcc cttcgatgcg gcatcgagag ccggatccgt ccggggtgcg ccagacggtt
     2821 ggttgctcct gtagagcgac aaagcgggcc tctctcctcc ctagctcgct tattcgagtc
     2881 cgccacgagt ctccacgtgg cggacttttt ttttggatga tgaccggcgg cagacgccga
     2941 cttgttcagg cgggtcgcga agttcagcgc gcgaacccgc cgcgcaaacc tcgcccggat
     3001 tacctgactc gcactcggag aggatccaag ccgacctcgg ccggcgtctt ctgcatccca
     3061 ggcggcctca ttgcgctgaa cgtggtgtcg ctactggtgc gcgagccgaa atccgcttgg
     3121 atctgccagc cgtcaggccg gcagtgttcc ggcatcgagc gctcgcgcgc ccgcgtcgcc
     3181 ctcggcgttc agcaccagcc gcacgaggtc cggaacgcgc cgcacctgca gcttgtccat
     3241 cacgcgtgcc ttgtacacct cgacggtgcg cgcgctgatg ccgagatcgg cagcgatctc
     3301 gcggttgtgc ttgccggcga cgacctgcga cagcacctgg cgctcgcgct gcgtgaggcg
     3361 cttcattcgc tccgccaggt cctgcgcttc ggcctcgtgc gcggcgcgcc gcgcatcctc
     3421 ggcaagcgcg gcgccgacca gttcgaccag ccgatcgttg tcgatcggct tctcgatgaa
     3481 atcgaacgcg cccgccttca gcgcgttgcg cgcggtgccg acgtcgccgt
//
LOCUS       DBNHBY010000322        17596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_559985, whole genome shotgun sequence.
ACCESSION   DBNHBY010000322 DBNHBY010000000
VERSION     DBNHBY010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 17596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_559985"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2711
                     /locus_tag="AC16HB_07835"
     CDS             <1..2711
                     /locus_tag="AC16HB_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HzsA-related protein"
                     /protein_id="HIR5606740.1"
                     /translation="DATEPQVSYDGKKIVFALNCPTSNTSMINGQAACTGRWNIWEYD
                     MSTGGFAGGTFRRLTDSAQDDDVDPAYLPAGRGFVFSSNRQTKSKTAQALGRTYFALD
                     EYERERVFNLHTMDATGKSIQQISFNQSHDRNPVVRPNGDIMFSRWDHVAGRNHFKVF
                     RVKPDGTDMFVLYGAHSEGNSFLHPRDMDPNGPYKGFVSSDLMPLSGTHEGGALVFID
                     AANYSEQNTPASSTVPRIGGQKQATAQSLSMERGISMYGRITTPFPLWDGTNRVLLSF
                     APCEVTKNGVVVSCQMLTAEELARLNDENRAPADVQADTVQDNVPPSYAIYMFDPAQQ
                     TFRIVAAPPAGFMYTDPIPLQARPEPNATDPTIVDAALAQQNLALIEVRSVYDTDELG
                     RMGEGMLAGADLPAGCTVGIAKTSPTDPNDTRAQVADLVKIKDPADPAYTCAPARFIR
                     ATRAVAPPSSTMGLRSAIGETDFEQQQILGYAPIEPDGSFKLQIPADTPIALFVVDAK
                     GRAFQSHTNWIQARPGERRTCDGCHSPRRGAALNSGAVVNAMPAGVKTAMSSAHQSGE
                     TMASTRARLDASALNLTNDPVYADVWADATKPGVRVRASISLRYTGNPNPADDLVTAV
                     PANGIINYPEHVQPLWTRDRGANTCTNCHSDPAKLDLRATIAGTGRIVSYESLMVGRP
                     LLDANGQPVTRIEDGVPVVQRAPALVDVGASEGEATGLARKSRLIEIIFGERLKASSA
                     ALSEHPNPPATAPNHATLLNAAEKRLVSEWIDLGGQYYNDPFNGSSGVRTVTALSQAA
                     FEASVLPILRSTCLACHQPQGSSATSTDFRNNRFVLTGDADGDFNVTLTMISDTCNPA
                     ANYLLAKPSTIPHPSGATGQTAAVLPVASSSYTTIANWIRTGCPTP"
     gene            2720..3316
                     /locus_tag="AC16HB_07840"
     CDS             2720..3316
                     /locus_tag="AC16HB_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606741.1"
                     /translation="MLRALAIATTLALAACGGGAGNPFGNPPLVSNPPATSSKKLSFV
                     YFQRCVHPIFLAQLQTNINGVVATNSCAGSGCHDNASGTGGAFRVIASAQTVDVTDPA
                     NTADAIRAMDIYKNYYSAQGEVVFDAPLSSRLLIKPLVLGVLHGGGLIFTSVQDPNVK
                     LIEYWISHPVPQGQDEFSTAAFNMFTPPDPKAGTCNTD"
     gene            3301..4059
                     /locus_tag="AC16HB_07845"
     CDS             3301..4059
                     /locus_tag="AC16HB_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5606742.1"
                     /translation="MQYGLIRRSWLRWFAVLWLVLACATVHAKDAERVQIADPYIELR
                     TGPGRGYPIFHVAARQEWIEIELRHTDWYRVRTENGKVGWVQRQQLETTLTEAGGKKT
                     FRDIALDDYLRRRLEFGAAWGHFKSEPVLKIWTGYKLADTIAVEATLGQVQGLFSGTD
                     FWHLNLLAEPFSDRRLAPFVGIGVGKFKNIPVASLVGAATTDAKLANAMIGARFHLTE
                     RFVARVDYTLYTAFLSDRRSSEYRAITAGLSFFF"
     gene            4073..4696
                     /locus_tag="AC16HB_07850"
     CDS             4073..4696
                     /locus_tag="AC16HB_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HIR5606743.1"
                     /translation="MPTRILATALLTLALGAATLPSLAQQVIEPNVDRRDVRVPRIPS
                     NDIELGAFAGTYSTQNFGSSLVAGVRLGYHITEDVFVEGTYAATRVTDQDFRLILPGG
                     IFPSEKQTLSYYNVSAGYNVLPGEVFIGRNTAKATALYLIGGIGSTRFLEQRRQTFNF
                     GLGWRMFLRDWAALQVDMRDHVFSLDILGKRRNTQNLELSAGVTFFF"
     gene            4711..5196
                     /locus_tag="AC16HB_07855"
     CDS             4711..5196
                     /locus_tag="AC16HB_07855"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5606744.1"
                     /translation="MIRILVALLLSASCSVVAAVTTERAAPDFTLRTVDGPNLRLQEQ
                     RGRVVLVNFWATWCGPCRQEMPHLNRLYEKYRTAGFTLLGVNIDDDARNAAGLSTKLG
                     LKFPVLLDTDKKVSQLYDLASMPSTVLIDRDGRVRFIHLGYKDGYEVTYEKQIRELLK
                     E"
     gene            5193..5411
                     /locus_tag="AC16HB_07860"
     CDS             5193..5411
                     /locus_tag="AC16HB_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HIR5606745.1"
                     /translation="MKTLIVAGALAALLAGCAVEPWVKPYERERLADPIMRAARDPLA
                     DKHRAHVYEVREGARGATGVQGGGCGCN"
     gene            5398..6516
                     /locus_tag="AC16HB_07865"
     CDS             5398..6516
                     /locus_tag="AC16HB_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HIR5606746.1"
                     /translation="MAATDVLRTLRQRLLDLMAGVLGGALATHSAGAAQLPEDRADAM
                     MHVYDGGGVRAWGPALLVRKSLADKVSLTASYYADIVSNASIDVVTTASPFKETRNEY
                     SVGADYAYRDALMSVSLATSREPDYTANTVNFDVAQDVFGGMTTVALGFTRAADTVKR
                     HNDPTFNERATHWRYRLGATQILTPRWLASVNTEIVADDGYLGSPYRAARVFGAAVPE
                     RLPSTRTSRAVQFRVVGDMGHRDAVRAEYRYFWDTWNIRAHTAELGYSRYLGADLLAD
                     GYLRFNSQQKALFYSDNFTSETTYITRNRQLGTFTSTGVGFKASYTAKRQPGRYEIKV
                     NTAYELLHFKYSDFTDIRTGSPYSFNANVLEVFVTANF"
     gene            6525..7070
                     /locus_tag="AC16HB_07870"
     CDS             6525..7070
                     /locus_tag="AC16HB_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR5606747.1"
                     /translation="MLKRILSTLTATLAIAASAYAADVATVTTGRPDSASLDSRIEEA
                     KGDVIRLNRDLLVLEEELLFPANTQVAVFVSMDVGKLFALDSVQVKLDDKIVANYLYT
                     PAEVQALHRGGVQRLYLGNLKSGRHELVAFFTGQGPHERDYKRGATLTIDKGTEPKYV
                     ELQIKDSQGKLQPEFEVKVWQ"
     gene            7074..8603
                     /locus_tag="AC16HB_07875"
     CDS             7074..8603
                     /locus_tag="AC16HB_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606748.1"
                     /translation="MSSIAFKFSEVLRRSVGAMVLALCAGAAAADGVIKAPHYGDSLF
                     YFFQDRYFTSVTNLMVSQHFARLPQHDDEAEVLRGGLFLSYGLHREAGQVFTQLIEKG
                     APPPVRDRAWFYLAKIRYQRGYLAEAEDAIGRIEKVLPGDLEEERVLLRAQLLMARQD
                     YAGAAQVLAAVKPDQGFAGQYVRYNLGVALIKSGETARGIALLDELGRAPSKNEEQRG
                     LRDKANVALGFALLQENTPQQARAYLERVRLSGLQANKALLGFGWAAAAMNQPKAALV
                     PWMELAQRDAGDSAVLEAKIAVPYALAELGAFGQSLKLYEDAVAVFERESTNLDESVA
                     AIRAGKLVEDLLRLNPGDEMGWFWNIQQLPQMPHAGHLAQVLAQHEFQEAFKNYRDLR
                     FLERNLREWQDRLGVFGDMLANRRHAYEERLPQVREKERALGIAELERRGRDLAAELV
                     RVERDADVHALADAKERELIERLARVRDILERDGSEPEVVAARDRYRRVAGALSWRLT
                     M"
     gene            8637..8954
                     /locus_tag="AC16HB_07880"
     CDS             8637..8954
                     /locus_tag="AC16HB_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606749.1"
                     /translation="MKALEAELERARVLDAALAQAQRDEPARFDRLAGRIAQLSQQVQ
                     AQIPRVERLAAEQQRYVQDLAAAELMRQKERVAEYANQARFAVAQIYDRAYVAKDAGN
                     ATR"
     gene            8941..11751
                     /locus_tag="AC16HB_07885"
     CDS             8941..11751
                     /locus_tag="AC16HB_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606750.1"
                     /translation="MRPGRHARIGITLLVAGGLAACASTNRDAADNAPTLKSLEQRQV
                     AIAADQGIDTNEEKAIAAYKEFLKAAPRDGQRQEALRRLGDLEMDSADARSAAGQAAA
                     PDYRGAIARYQDFLRTYPNDPGNDRVLYQLARAYELGGQLETALATLDTLVQRYPQTR
                     YRDEAQFRRGELLFTTRDYGGAERAFTTVLASQTRTPYHERSLYMHGWSLYKQGRLDE
                     GLKSFFRVLDLKLAGSDDDADLAKLPGLTRADLELVEDTFRVTSLSLQNLQGAESIAA
                     YIDSPLRRGYEFRVYQELGELYLKQERIKDAADTFAAFARRNPLHPQAPLLQARVIDI
                     YQGNGFATLALEGKREYVQRYGSRSEFRSTNPAGWERAQPLVKLHLAELAQHHHAAAQ
                     KSKKSEDYQEAARWYREYLASFPQDPQAAQSNFLLAELLFEDRKFDQAAVEYEKAAYA
                     YPPHAKSADAGYAALLAYAEQDKRGGAADGGKLKLAAIESELRFARSFPRDPRAGAVL
                     TNAAEKLYALRDVERAEAVAQQVLALDPSVGAAQRRVASLVLAHAAFDRGAFDQAEKM
                     YGAVLGFTDDKDASRKDLTERLAASIYKQGEQARSAGKLREAVAHFERVAAVAPQSTV
                     RATAQYDAAAALLTLKDWDRAARTLEDFRRSFPSHPLAQDAAAKLAVAYTEKGQWAQA
                     AAEFDRISTSQKDPQLARAALWQSAELYEKGGATAAATKAYDRYAKAYREPFEANLEA
                     RYRLAQLAHKEGNAARHLAGMRDVLRADQTAGAARTDRTRYLGAQAALALAEPLRDAY
                     RAVALVEPLQKNLKLKKARMEEVLKAYALAADYGVAEVATAATFQTAELYGEFGKAML
                     GSQRPKGLKKVELEQYNVLLEEQAFPFEEKAIELHEANARRSAKGIYDKWVQASYAAL
                     GKLRPVRYAKTERSEGTIDAIR"
     gene            11822..12406
                     /locus_tag="AC16HB_07890"
     CDS             11822..12406
                     /locus_tag="AC16HB_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5606751.1"
                     /translation="MATAPAAPVAAPAAAAPPKVEPAPVVSASVQRAFDDARRALQAG
                     RIDDAERGFVALTQSNPELGGPHANLGLLHRQAGKLAEAAADFELAVKANPQQPVYLN
                     QLGITYRQLGEFQKARDAYERAILLAPDYAAAHLNLGILYDLYLWDGARALAAYDRYL
                     ALASDEDGKVKKWIVDLRNRKPQPPTLLSRKEQQ"
     gene            12403..12708
                     /locus_tag="AC16HB_07895"
     CDS             12403..12708
                     /locus_tag="AC16HB_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606752.1"
                     /translation="MIRTLALPIVMAMFAAPALAQDRAAIDRAQIIGNRELPKVLYIV
                     PWKKPLPGDLAGRPVTSVLDEALAPIDRDVFRRQLQYDALTRVEPAAKTADGKQEVF"
     gene            12737..13387
                     /locus_tag="AC16HB_07900"
     CDS             12737..13387
                     /locus_tag="AC16HB_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5606753.1"
                     /translation="MLTFGTMVKFFQDCGLFIYPSLLIMALGLSVAIERFIFLARARR
                     ENSKAWRDILPLLKAGHYKEVHGMASKSDTAIGLIVNNGLARMGSARRREDIDAAMEE
                     GMMEIVPRLEKRTHYLATFANVITLVGLLGTIIGLIKAFTAVAQVNPAEKAELLSASI
                     SIAMNNTAFALMVAIPFLLIHAFLQARATEIVDSLEAAKISFLNLVHRAPEAAPAR"
     gene            13390..13890
                     /locus_tag="AC16HB_07905"
     CDS             13390..13890
                     /locus_tag="AC16HB_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR5606754.1"
                     /translation="MRVRRLRRRPAELDITAFLNLIVVLVPFLLSTAVFTRLSIMDLK
                     LPAQSSNFEQLKGDLQLEVVIRPDALDVGDKIGGLIQHIPNVASGYDVKTLSALIQQL
                     KLRFPDKSSASILAQPDTPYDVLVQVMDAVRAGRTVQGAQIVRTDFFPEISIGDAPVR
                     AGQRKS"
     gene            13887..14381
                     /locus_tag="AC16HB_07910"
     CDS             13887..14381
                     /locus_tag="AC16HB_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5606755.1"
                     /translation="MNKTPLEKRAERRDRNKSLVDLNLVSLIDIFTILIFFLLSHSAE
                     VEVLPSSKAVKLPESSAEKTPRDTLVVVVNNSDILVQGRKVASVADVMRAEDDLIAPL
                     KAELDYQSGRQVIRLENEAQKNAITIMGDKDIPYQLLRKVMVTCARANFSDVAFAVRR
                     KAET"
     gene            14399..15457
                     /locus_tag="AC16HB_07915"
     CDS             14399..15457
                     /locus_tag="AC16HB_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5606756.1"
                     /translation="MHRPAVLPWVGVEIDERRFRRILQRVLLTTAILAVALPWIPVPK
                     LDRTKIESLPAPMARLLLENKPMPVIPAKVAPAPKVEADQKESPPRADDLKLDPKKAP
                     VPEARRPEPNKPPGELLDNARRKASGVGLLAMKNEIADLRGAPAAVQLKADIKPGPGV
                     GIGSGPGVGAGTDPGVPVRALITSNATNGSGGINTAAYSRDTGGGGLAGRSTTLVEGV
                     AGGGGGGGYGGGGGVRNGGGRGSGTGAGGVLQRSGSGKASRSIEEIKLVFERNKGAIY
                     AIYNRALREDPALQGKVVLELTIAPTGNITNLRILSSELKAQELEQKLLARIRQFDFG
                     AKDVEVMVVSWPVDFLPS"
     gene            complement(15473..17497)
                     /locus_tag="AC16HB_07920"
     CDS             complement(15473..17497)
                     /locus_tag="AC16HB_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5606757.1"
                     /translation="MSSHPHLLSPLDFGFLTLGNRIVMGAMHTRLETLERPAERLAAF
                     YAARARGETGLLLTGGYAPSPEGRIEPDGPLLNDESQLDLHRAVCEAVHRAGGRVALQ
                     ILHTGRYAKLPECVGPSAIRARINRFTPRALTTDEVWRTIGDFARTAALARRAGYDGV
                     EIMASEGYLINEFTAPATNRRTDAFGGSFDNRARLPVEIVKAVRKAAGADFLLIYRIS
                     SIDLVEGGVPAAETVELARRVQDVGTNILNTGIGWHESPVPTIAASVPRAAWSFAIRR
                     VKQAVTIPVIASNRINDPDVAESLLAEGVADLVSMARPLLADPDFARKVRESRADEIN
                     TCIACNQSCLDRIFTERAATCLVNPRAGREIEFSEAPAARRKRIAVVGAGPAGMAFSI
                     NAAERGHAVHLYEAASELGGQLNMARVVPGKSEFNEMLRYFRVRLERLGVPIRLGTRV
                     DADSLAREAFDAIVLATGVKPRVPKFAGIDHPKVISYVDVLTRRKQVGRRVAIIGAGG
                     IGFDVAEFLLGDADESRLPDRFMDAWNVDPSARSGGLHGATRAKSPAPKREVHMFQRK
                     IDKLGANLGKTTGWILKARLQEAGVQMVAGATYLAIDDAGLHYAVGGETRVLDVDHVV
                     LCAGQESERDLYEDLKARGIGAHLIGGADVAAELDAARAIEQATRLAVAV"
BASE COUNT         3090 a         5845 c         6023 g         2638 t
ORIGIN      
        1 gtgacgccac cgagccgcag gtgtcatacg acggcaagaa gatcgtcttc gcgctgaatt
       61 gtccgacctc gaacacgtcg atgatcaacg gccaggctgc ttgtaccggc cgctggaaca
      121 tctgggaata cgacatgagc accggcgggt tcgccggcgg cacttttcgc cgcctgacag
      181 actcggcgca ggacgacgac gtcgaccccg cgtacctgcc ggccggccgg ggcttcgtgt
      241 tctcttcgaa ccggcagacg aagtcgaaga cggcgcaggc gctcgggcgc acgtacttcg
      301 cgctcgacga atacgagcgc gaacgcgtgt tcaacctgca cacgatggat gccaccggca
      361 agagcatcca gcagatctcg ttcaaccaga gccacgaccg caacccggtc gtgcggccga
      421 acggcgacat catgttctcg cgctgggacc acgttgccgg gcgcaaccac ttcaaggtct
      481 tccgcgtcaa gcctgacggc accgacatgt tcgttctgta cggcgcacac agcgaaggca
      541 acagcttcct gcatccgcgc gacatggacc cgaacggccc gtacaagggc ttcgtgtcgt
      601 cggacctgat gccgctgtcg ggcacccacg agggcggcgc gctggtgttc atcgacgcgg
      661 ccaactactc ggaacagaac acgccggcca gcagcacggt gccgcgcatc ggcgggcaga
      721 agcaggccac tgcgcaatcg ctgagcatgg agcgcggcat ctcgatgtac gggcgcatca
      781 cgacgccctt tcccctctgg gacggcacca atcgggtgct gctgtcgttc gcgccgtgcg
      841 aggtcacgaa gaacggcgtc gtcgtttcgt gccagatgct gaccgcggag gaactcgcgc
      901 ggctaaacga cgagaaccgt gcgccggcag atgtgcaagc cgacacggtg caggacaacg
      961 ttccgccgtc gtacgcgatc tacatgttcg atccggcgca gcagacgttc cgcatcgtcg
     1021 ccgcgccgcc ggcgggcttc atgtacacgg atccgattcc gctgcaggcg cggcccgagc
     1081 cgaacgcgac cgatccgacc atcgtcgatg cggcgctggc gcagcagaat ctggcgctga
     1141 tcgaggtgcg cagcgtgtac gacaccgacg aactcggccg catgggcgag gggatgctgg
     1201 ccggcgccga cctgccggcg ggctgcacgg tcggcatcgc caagaccagc ccgaccgatc
     1261 cgaacgacac gcgcgcgcag gttgccgacc tggtgaagat caaggatccg gctgatcctg
     1321 cctacacctg cgcgccggcg cgcttcatac gcgccacgcg cgcggtcgcg ccgccatcga
     1381 gcacgatggg tctgcgcagc gcgatcggcg aaaccgactt cgagcagcag cagattctcg
     1441 gctatgcacc gatcgagccc gacgggtcgt tcaagctgca gattccggcg gacacgccga
     1501 tcgcgctgtt cgtcgtcgat gccaaggggc gcgcgttcca gagccacacc aactggatcc
     1561 aggcgcgccc gggcgagcgg cggacgtgcg acggttgcca cagcccgcgg cgcggggcgg
     1621 cgctcaactc cggggcggtg gtcaacgcaa tgccggccgg cgtgaagacc gcgatgtcga
     1681 gcgcgcacca gtcgggcgag acgatggcct cgacgcgcgc gcggctcgat gcgagcgcgc
     1741 tgaatctgac caacgatccg gtctacgcgg acgtctgggc cgacgcgacc aagcccggcg
     1801 taagggtgcg cgcttcgatc tcgctgcgtt acaccggcaa cccgaacccg gccgacgatc
     1861 tagtcactgc cgttccggcc aacggcatca tcaactatcc cgagcacgtc cagccgctgt
     1921 ggacgcgcga tcgcggcgcc aacacctgca ccaactgtca cagcgacccg gccaagctcg
     1981 atctgcgcgc gaccattgcc ggtaccggcc gcatcgtgtc gtacgagagt ctgatggtcg
     2041 gcaggccgct gctcgacgcg aatggccagc ccgtcacgcg catcgaggac ggcgtgcccg
     2101 tggtgcagcg cgcgccggcg ctggtcgatg tcggtgcgag cgagggcgaa gccacggggc
     2161 tcgcgcgcaa gagccggctg atcgagatca tcttcggcga gcggctgaag gcgagcagcg
     2221 ccgcgctctc cgaacatccg aacccaccgg cgacagcgcc gaaccatgcg acgctgctca
     2281 atgcggcgga gaagcggctg gtgtcggagt ggatcgacct cggcggtcag tactacaacg
     2341 acccgttcaa cggcagcagt ggcgtacgta cggtgaccgc gctgagccag gcggcgttcg
     2401 aggccagcgt gctgccgatc ctgcgttcga cgtgcctggc ctgccatcag ccgcagggca
     2461 gcagcgcgac gagtaccgat ttccgcaaca accgcttcgt gctgaccggc gacgccgacg
     2521 gcgacttcaa cgtcacgctg acgatgatct ccgacacctg caatccggcc gcgaactacc
     2581 tgctcgccaa gccatcgacc attccgcatc ccagtggcgc gaccggtcag accgccgcgg
     2641 tgctgccggt ggccagcagc agctacacca cgatcgcgaa ctggatccgg acggggtgcc
     2701 cgacgccatg acgtgcagcg tgttgcgcgc acttgcgatc gcgacaacgc tcgcgctcgc
     2761 cgcctgcggc ggcggcgcgg gcaacccgtt cggcaacccg ccgctggtga gcaatccgcc
     2821 agcgacttcc agcaagaagc tgtccttcgt ctacttccag cgctgcgtcc atccgatctt
     2881 cctcgcacag ttgcagacga acatcaacgg cgtcgtcgcg acgaacagtt gcgcgggatc
     2941 gggctgccac gacaacgcga gcggcaccgg gggtgccttc cgcgtgatcg cctcggcaca
     3001 gaccgttgac gtcacggatc cggcgaacac ggcggacgcc atccgcgcca tggacatcta
     3061 caagaactac tactcggcgc agggcgaggt cgtgttcgat gcgccgcttt cgagtcgcct
     3121 gctgatcaag ccgctggtgc tcggcgtcct gcacggtggc ggcctcatct tcaccagcgt
     3181 gcaggacccg aacgtcaagc tgatcgagta ctggatcagc catccggtgc cgcaaggcca
     3241 ggacgagttc agcaccgctg cgttcaacat gttcacgccg cccgacccga aagccggaac
     3301 gtgcaatacg gactgattcg tcgatcgtgg ctgcgctggt ttgcggtcct gtggctggta
     3361 cttgcatgcg ccaccgtgca tgcgaaggat gccgagcgcg tgcagatcgc cgatccgtac
     3421 atcgaactgc gcaccgggcc gggccgtggg tatccgatct tccacgtcgc ggcgcggcag
     3481 gagtggatcg agatcgagct gcgccacacc gactggtacc gcgtgcgcac cgaaaacggc
     3541 aaggtcggct gggtgcagcg ccagcaactc gagaccacgc tgaccgaggc gggcggcaaa
     3601 aagacctttc gcgacatcgc gctcgacgac tacctgcgcc gccgcctcga gttcggcgcg
     3661 gcctggggtc acttcaagtc cgagccggtg ctgaagatct ggaccggcta caagctcgcc
     3721 gacacgatcg cggtcgaagc cacgctcggg caggtgcagg ggttgttctc gggcaccgac
     3781 ttctggcacc tgaacctgct cgcggagccg ttctcggatc gccggctcgc gccgttcgtc
     3841 ggcatcggcg tgggcaaatt caagaacatt ccggtcgcga gcctggtcgg cgcggcgacc
     3901 accgacgcca agctggccaa cgcaatgatc ggcgcgcgct tccacctgac cgaacgcttt
     3961 gtcgcgcgcg tcgattacac gctctacacg gcgttcctgt cggatcgccg cagcagcgaa
     4021 taccgcgcca tcacggccgg gctctcgttc ttcttctaga cacagaccac cgatgccaac
     4081 aagaatcctt gccaccgcac tgctgacgct ggcgctcggc gccgccacgc tgccgtccct
     4141 cgcgcagcag gtgatcgaac ccaacgtcga ccggcgcgac gttcgtgtgc cgcgcattcc
     4201 gtcgaacgac atcgagctcg gcgcatttgc cggaacctac agcacgcaga acttcggctc
     4261 gagcctcgtc gccggcgtgc ggctcggcta tcacatcacc gaggacgtct tcgtcgaggg
     4321 cacctacgcg gcgacccgcg tcaccgacca ggatttccgg ctcattcttc ccggcggaat
     4381 cttcccgagc gagaagcaga cgctgtccta ctacaacgtc tcagccggct acaacgtgct
     4441 gccgggcgaa gtcttcatcg ggcgcaacac cgcgaaggcc acggcgctgt atctgatcgg
     4501 cggcatcggc agcacgcgct tcctcgagca gcggcgccag acgttcaact tcggcctcgg
     4561 gtggcgcatg ttcctgcgcg actgggccgc gctgcaggtc gacatgcgcg accacgtgtt
     4621 ctcgctcgac atcctcggca agcgacgcaa tacgcagaac ctcgagctca gcgccggggt
     4681 gacgttcttc ttctgaggga gcgcgcgacg atgatccgga tcctggtggc gcttctgctg
     4741 tccgcgtcgt gcagcgtcgt ggcagcggtg acgaccgagc gcgccgcgcc cgacttcacg
     4801 ctgcgcacgg tcgacggccc gaacctgcgt ctgcaggaac agcgcggccg ggtcgtgctg
     4861 gtgaacttct gggccacgtg gtgcggcccg tgccgccagg agatgccgca cctgaaccgt
     4921 ttgtacgaaa agtaccgcac ggccggcttc acgctgctcg gcgtgaacat cgacgacgac
     4981 gcgcgcaatg cggccgggct gtcgaccaag ctcgggctga agttcccggt gctgctcgac
     5041 accgacaaga aggtgagcca gttgtacgac ctggcgtcga tgccgtcgac cgtgctgatc
     5101 gatcgcgatg gccgcgtgcg cttcatccac ctcggctaca aggacggcta cgaggtcacc
     5161 tacgaaaagc agatccggga gctgctgaag gaatgaagac actgattgtt gccggtgctc
     5221 tggccgcgct gctcgcgggg tgcgcggtcg agccgtgggt caagccgtac gagcgcgagc
     5281 ggttggccga tccgatcatg agggccgcgc gcgatccgct ggcggacaag catcgcgcgc
     5341 acgtgtacga ggtgcgcgag ggcgcgcgcg gcgccaccgg tgtgcaagga ggcggctgtg
     5401 gctgcaactg acgtgttgcg aacgctgcgg cagcggctgc tcgacctgat ggccggcgtg
     5461 ctgggcggcg cactggcgac ccacagcgcc ggcgcggcac agctgccgga ggatcgcgcc
     5521 gacgccatga tgcacgtgta cgacggcggg ggcgtgcgcg cctggggccc ggcgctgctc
     5581 gtgcgcaaga gccttgccga caaggtgtcg ctgacggcgt cgtactacgc cgacatcgtc
     5641 agcaatgcgt cgatcgacgt ggtcacgacc gcgagcccgt tcaaggagac gcgcaacgag
     5701 tacagcgtcg gcgcggatta tgcgtaccgc gatgcgctga tgagcgtgtc gttggcgacc
     5761 agccgcgagc ccgactacac ggccaacacg gtcaacttcg acgtcgcgca ggatgtgttc
     5821 ggcggcatga cgaccgtcgc cctcggcttc acgcgcgcgg ccgacacggt caagcggcac
     5881 aacgacccga ccttcaacga gcgcgcgacg cactggcgct accggctcgg cgccacgcag
     5941 atcctgacgc cgcgctggct ggcgagcgtg aacacggaga tcgtcgccga cgacggctat
     6001 ctcggcagcc cgtaccgcgc cgcgcgcgtg ttcggcgccg cggtgccgga gcggctgccg
     6061 agcacgcgca ccagccgcgc ggtgcagttc cgcgtggtcg gcgacatggg tcaccgcgac
     6121 gccgtgcgcg ccgagtatcg ctacttctgg gacacctgga acatccgcgc gcacacggcg
     6181 gagctggggt acagccgcta cctcggcgcc gacctgctcg ccgacggtta cctgcgcttc
     6241 aactcgcagc aaaaggcgct tttctacagc gacaacttca cgagcgagac cacgtacatc
     6301 acgcgcaacc gccagctcgg cacgttcacc agcaccggcg tcggcttcaa ggcgtcgtat
     6361 accgcaaagc gccagccggg tcgctacgag atcaaggtga acaccgcgta cgaactgctg
     6421 cacttcaagt acagcgactt caccgacatt cgcaccggca gcccgtactc gttcaatgcc
     6481 aacgtgctgg aagtcttcgt cacggccaac ttctgaggtc agccatgctg aaacgaatcc
     6541 tctcgactct gactgcgacg ctcgcgatcg ccgcttccgc gtacgcggcc gatgtcgcca
     6601 cggtgaccac gggccgcccg gattcggcca gcctcgacag ccgcatcgag gaggccaagg
     6661 gtgacgtgat ccggctgaac cgcgatctgc tggtgctcga ggaggaactg ctgtttccgg
     6721 ccaatacgca ggtcgccgtg ttcgtgtcga tggacgtggg caagctgttc gcgctcgact
     6781 cggttcaggt gaagctcgac gacaagatcg tcgccaacta cctgtacacg cccgccgagg
     6841 tgcaggcgct gcatcgcggc ggtgtgcagc ggctgtacct ggggaacctg aagtccggac
     6901 ggcacgagct ggttgcgttc ttcaccggcc aagggccgca cgaacgcgac tacaagcgcg
     6961 gcgcaacgct gacgatcgac aagggcaccg agcccaagta tgtcgagctg cagatcaagg
     7021 actcgcaggg caagctgcag ccggagttcg aagtcaaagt gtggcagtga ccggtgtcga
     7081 gcatcgcctt caagttcagt gaagtgctgc gccgaagcgt cggtgcgatg gtgctcgcgc
     7141 tgtgcgccgg cgcggcggcg gccgacggtg tgatcaaggc gccgcactac ggcgacagcc
     7201 tgttctactt cttccaggac cgctacttca cctcggtgac gaacctgatg gtgtcgcagc
     7261 acttcgcgcg gctgccgcag cacgacgacg aggccgaggt gctgcgcggc gggctgttcc
     7321 tctcgtatgg gctgcatcgc gaagcagggc aggtcttcac gcaactgatc gaaaagggtg
     7381 cgccgccgcc ggtgcgcgac cgcgcctggt tctatctcgc caagatccgc taccagcggg
     7441 gctacctcgc cgaggcggag gatgcgatcg gccgcatcga aaaggtgctt ccgggcgatc
     7501 tcgaggaaga acgcgtgctg ctgcgcgccc agttgctgat ggcgcggcag gactatgcgg
     7561 gcgctgcgca agtgctcgcc gcagtgaaac ccgaccaggg attcgctggc cagtacgtgc
     7621 gctacaacct gggcgtggct ctgatcaaga gcggcgagac ggcgcgcggc atcgcgctgc
     7681 tcgacgagct tggccgtgcg ccgtcgaaga acgaagaaca gcgcggcctg cgcgacaagg
     7741 ccaacgttgc gcttggcttc gctttgctgc aggagaacac gccgcagcag gcgcgcgcgt
     7801 atctggagcg cgtgcggctg tcgggattgc aggccaacaa ggcgctgctc ggcttcggct
     7861 gggcggccgc ggcgatgaac cagccgaaag cggcgctggt gccctggatg gaactggccc
     7921 agcgcgacgc cggggacagc gccgtgctcg aggccaagat cgcggtgccg tatgcgctgg
     7981 cggagctggg cgcgttcggg cagtcgctga aactgtacga ggacgcggtc gcggtgttcg
     8041 aacgcgagag cacgaacctc gacgaatcgg tcgccgcgat ccgcgccggc aagctcgtcg
     8101 aggacctgct gcggctcaat cctggtgacg agatgggctg gttctggaac atccagcagt
     8161 tgccgcagat gccgcacgcc gggcatctcg cgcaggtgtt ggcacagcac gaattccagg
     8221 aggcgttcaa gaactaccgc gacctgcggt tcctcgaacg caacctgcgc gaatggcagg
     8281 acaggctcgg ggtgttcggc gacatgcttg ccaaccggcg ccatgcgtac gaagaacggc
     8341 tgccgcaggt gcgcgagaaa gagcgcgcgc tgggcatcgc ggagctggag cgccgcggcc
     8401 gggatctggc ggcagaactc gtgcgcgtcg agcgcgacgc cgacgtgcac gcgctggccg
     8461 acgcgaagga gcgcgagctg atcgagcggc tggcgcgcgt gcgcgacatc ctcgagcgcg
     8521 acggcagcga gccggaggtg gtcgcagcgc gcgaccgcta tcgccgcgtc gcgggtgcgc
     8581 tgagctggcg actgacgatg tagttccatc cgcggctctg ggacgcgaag aagaacatga
     8641 aggcactcga agccgaactg gaacgggcgc gcgtgctcga cgccgcactg gcgcaggcgc
     8701 agcgcgacga gcccgcgcgc ttcgaccggc tcgccggccg catcgcgcag ctttcgcagc
     8761 aggtgcaggc gcagattccg cgcgtcgaac ggctcgcagc cgaacagcag aggtacgtgc
     8821 aggacctggc ggcagccgaa ctgatgcggc agaaggagcg cgttgccgag tacgcgaacc
     8881 aggcgcgctt cgccgtggcg cagatctatg accgggccta cgtggccaag gatgcgggca
     8941 atgcgacccg gtagacacgc tcgcatcggc atcacactgc tcgtcgcggg cgggcttgcc
     9001 gcgtgcgcaa gcacgaacag ggatgctgcc gacaacgcgc cgacactgaa gagcctggaa
     9061 cagcggcagg tcgcgatcgc tgccgatcag ggcatcgaca cgaacgaaga gaaggcgatc
     9121 gccgcctaca aggagttcct gaaggctgcg ccgcgcgatg gacagcgaca ggaagcactg
     9181 cgccggctcg gcgacctgga aatggacagc gcggatgcgc gcagcgcggc cggccaggca
     9241 gccgcgcccg actaccgcgg cgcgatcgcg cgctaccagg acttcctcag gacctatccg
     9301 aacgatccgg gcaacgaccg ggtgctgtac cagcttgcgc gggcgtacga gctcggcggc
     9361 cagctcgaga cggcgctcgc gacgctcgac acgctggtgc agaggtatcc gcagacccgt
     9421 taccgggacg aggcgcagtt ccgccgcgga gagctgctgt tcaccacgcg cgactatggc
     9481 ggtgctgaaa gagcgttcac caccgtgctc gcgagccaga cgcggacgcc gtatcacgag
     9541 cgctcgctct acatgcacgg ctggtcgctg tacaagcagg ggcggctcga cgaagggctg
     9601 aaatccttct tccgcgtgct cgacctgaag ctcgcgggca gcgacgacga tgccgacctg
     9661 gccaagctgc cgggactgac gcgggcggat ctcgaactcg tcgaagacac ctttcgcgtg
     9721 acgagcctgt cgctgcagaa cctgcagggc gccgagtcaa tcgccgccta catcgattcg
     9781 ccgctgcggc gcggctacga gttccgcgtg taccaggagc tcggcgagct gtacctgaag
     9841 caggagcgga tcaaggatgc cgcggacacc ttcgccgcgt tcgcgcgccg caatcctctg
     9901 cacccgcagg caccgctgct gcaggcgcgc gtgatcgaca tctaccaggg caacggcttt
     9961 gccacgctgg cgctcgaagg caagcgggaa tacgtgcagc gctatggcag caggagcgaa
    10021 ttccgcagta cgaacccggc cgggtgggaa cgcgcgcagc cgctggtgaa gctgcacctc
    10081 gcggagctgg cgcagcacca ccacgccgca gcgcagaagt cgaagaagag cgaggactac
    10141 caggaagcgg cgcgctggta tcgcgagtat ctggcatcgt ttccgcagga tccgcaggcg
    10201 gcacagtcga acttcctgct ggccgaactg ctgttcgagg accggaagtt cgaccaggcg
    10261 gccgtcgaat acgaaaaggc cgcgtatgca tatccgccgc acgccaagag cgccgacgcc
    10321 ggctatgcgg cgctgctcgc ctatgcggag caggacaagc gcggcggcgc cgcagacggc
    10381 ggcaagctca agctcgccgc gatcgaaagc gagctgaggt tcgcacgctc gttcccgcgg
    10441 gacccgcgtg ccggcgccgt gctgaccaat gcggccgaga agctgtacgc gctgcgcgat
    10501 gtcgagcgcg ccgaggccgt ggcgcagcag gtgctggcgc tcgatccgtc cgtcggtgcc
    10561 gcgcaacgcc gcgtggcatc gctggtgctc gcgcatgcgg ccttcgatcg cggcgcgttc
    10621 gaccaggcgg agaagatgta cggcgcggtg ctcgggttca ccgatgacaa ggacgcgagc
    10681 cgcaaggatc tgacggagcg cctggccgcg tcgatctaca agcagggcga gcaggcgcgc
    10741 tcggccggca agctgcgcga agcggtcgcg catttcgagc gcgtggcggc cgtcgcgccg
    10801 cagtcgacgg tgcgcgcgac cgcgcaatac gacgcggcgg cagcgctgct gacgctgaag
    10861 gactgggacc gtgctgcacg cacgctggag gacttccgtc ggagcttccc gagccatccg
    10921 ctggcgcagg acgcggccgc caaactcgcc gtcgcgtaca cggagaaggg ccagtgggcg
    10981 caggcggcgg ccgagttcga ccgcatttcc acctcgcaga aggatccgca gcttgcgcgc
    11041 gcggcactgt ggcagtcggc cgaactgtat gaaaagggcg gcgcgacggc ggcagcgacg
    11101 aaggcgtacg accgctatgc gaaggcctat cgcgagccgt tcgaagccaa tctcgaggcg
    11161 cgctatcggc tcgcccagct ggcacacaag gaaggcaatg cggcgcgcca cctggcgggc
    11221 atgagagacg tgctgcgcgc cgaccagacg gcgggcgcag cgcgcaccga ccgcacccgc
    11281 tatctgggcg cgcaggcggc gctggcgctc gcggagccgt tgcgcgacgc gtatcgcgcg
    11341 gtcgcgctgg tcgagccgct gcagaagaac ctgaagctga agaaggcgcg gatggaagaa
    11401 gtgctgaagg cctacgccct tgccgcggac tatggcgtgg ccgaggtcgc gacggccgcg
    11461 acgttccaga ccgccgagct gtacggcgaa ttcggcaagg cgatgctcgg atcacagcgg
    11521 cccaaggggc tgaaaaaggt cgaactcgag cagtacaacg tgctgctgga agagcaggcg
    11581 ttcccgttcg aggagaaggc catcgagttg cacgaggcaa acgcgcgccg cagcgcgaag
    11641 ggcatctacg acaagtgggt gcaggcgagc tatgccgcgc tcggaaagct caggccggtg
    11701 cgctatgcaa agacggagcg cagtgaaggg acgatcgatg cgattcgata gcgcagtgat
    11761 acttgcgttg ctggtgctgt gcgtcgccgc gacaggatgt gcgacacgcg ccgagccgcc
    11821 gatggcgacg gcgcccgccg cgccggtcgc ggcgcccgct gcggcggccc cgccgaaagt
    11881 ggagccggca ccggtcgtca gcgcaagcgt gcagcgcgca ttcgacgacg cgcgccgtgc
    11941 gttgcaggcc gggcgcatcg acgacgccga gcgcggattc gttgcgctga cccagtcgaa
    12001 ccccgagctc ggcggcccgc acgccaacct cggcctgctg catcggcagg ccggcaagct
    12061 ggccgaggca gccgccgact tcgaactcgc ggtgaaggcg aatccgcagc agcctgtcta
    12121 tctgaaccag ctcggcatca cctaccggca gctgggcgag ttccagaagg cgcgcgacgc
    12181 gtacgagcgc gccatcctgc tcgcacccga ctacgcggct gcccacctga acctcggcat
    12241 cctgtacgac ctgtatctgt gggacggtgc gcgcgcattg gccgcgtacg accgttatct
    12301 cgcgctggct tcggacgagg acggcaaggt gaagaaatgg atcgtcgacc tgcgcaatcg
    12361 caagccgcag ccgccgacgc tgctgagcag gaaggagcag caatgatccg aaccctggcg
    12421 ttgccgatcg tcatggcgat gttcgccgct ccggcgctgg cccaggaccg cgccgcgatc
    12481 gatcgcgcgc agatcatcgg caatcgcgag ctgccgaagg tcctctacat cgttccatgg
    12541 aagaaaccgc tgcccggcga tctcgccggc cggccagtga ccagcgtgct cgacgaggcg
    12601 ctggcgccga tcgaccgcga cgtgtttcgc cggcagttgc agtacgacgc gctgacgcgc
    12661 gtggagccgg ccgcgaagac ggccgacggc aagcaggaag tcttttgaac cagcacttga
    12721 aacaaggagg cccccgatgt tgacgtttgg aaccatggtg aagttcttcc aggactgcgg
    12781 gctgttcatc tatcccagcc tgctgatcat ggcgctcggc ctgtcggtcg cgatcgagcg
    12841 cttcatcttc ctcgcgcgcg cacggcgcga gaacagcaag gcctggcgcg acatcctgcc
    12901 gctgctgaag gccgggcact acaaggaagt gcacggcatg gcgtcgaagt ccgacactgc
    12961 gatcggcctg atcgtcaaca atggtctggc gcgcatgggc agcgcacggc ggcgcgagga
    13021 catcgacgcc gcgatggaag agggcatgat ggagatcgtg ccgcgcctgg aaaagcgcac
    13081 gcactatctg gcgaccttcg ccaacgtgat cacgctggtg ggcctgctgg gcacgatcat
    13141 cggcctgatc aaggcgttca ccgcggtcgc acaggtcaac ccggcggaga aggccgagct
    13201 tttgagcgcc agcatctcga tcgcgatgaa caacaccgcg ttcgcgctga tggtcgcgat
    13261 cccgttcctg ctgatccacg cgttcctgca ggcgcgcgcg accgagatcg tcgacagcct
    13321 ggaggccgcc aagatcagct tcctgaacct cgttcatcgc gcgcccgaag ccgcgcctgc
    13381 gcgctgacca tgcgcgtccg acgcctgcgc cgtagaccgg cggaactcga catcaccgcg
    13441 ttcctgaacc tgatcgtggt gctggtgccg ttcctgctgt cgacggcggt gttcacgcgc
    13501 ctgtcgatca tggatctcaa gctgccggcg cagtcatcga acttcgagca gctcaaaggg
    13561 gacctgcagc tcgaagtcgt gatccggccg gacgcgctcg atgtcggcga caagatcggc
    13621 gggctgatcc agcacatccc gaacgtcgct tcgggctacg acgtcaagac gctgtcggcg
    13681 ctgattcagc agctgaagct gcgctttccg gacaaatcca gtgcctcgat ccttgcccag
    13741 cccgatacgc cgtacgacgt gctggtgcag gtgatggacg cggtccgcgc cggtcgcacg
    13801 gtgcaggggg cgcagatcgt gcgcacggat ttcttcccgg agatctcgat cggggatgcg
    13861 ccggtccgtg cgggacagag gaagtcatga acaagacgcc gctcgagaag cgcgccgaac
    13921 ggcgcgaccg caacaagtcg ctggtcgacc tgaacctcgt gtcgctgatc gacatcttca
    13981 cgatcctgat cttcttcctg ctgtcgcatt cggccgaggt cgaagtgctg ccgagcagca
    14041 aggcggtgaa gctgcccgag tcgagcgccg aaaagacgcc gcgcgacacg ctggtggtcg
    14101 tggtcaacaa cagcgacatc ctggtgcagg ggcgcaaggt cgcgtcggtc gccgacgtga
    14161 tgcgtgccga agacgacctg atcgcgccgc tgaaggcgga actcgactac cagtcggggc
    14221 ggcaggtgat ccggctggag aacgaggcgc agaagaacgc gatcacgatc atgggcgaca
    14281 aggacatccc gtaccagttg ctgcgcaagg tgatggtgac ttgcgcgcgc gccaacttca
    14341 gcgacgtcgc gttcgcggtg cgcagaaagg ccgaaacgtg atgacggcca cgctagccat
    14401 gcatcgcccc gcggtgctgc cgtgggtcgg tgtggaaatc gacgagcggc gcttccgtcg
    14461 catcctgcag cgggtactgc tgacgaccgc gatccttgct gtcgcgctgc cgtggattcc
    14521 ggtaccgaag ctcgaccgca cgaagatcga gagcctgccg gcgccgatgg cgcggctgct
    14581 gctggagaac aagccgatgc cggtgatccc ggcgaaggtc gcgccggcgc cgaaggtcga
    14641 ggcagaccag aaggaatcgc cgccgcgcgc ggatgacctg aagctggacc cgaagaaagc
    14701 gcccgtgccc gaagcgcgcc ggccggagcc gaacaagccg cccggcgaac tgctcgacaa
    14761 cgcgcgccgc aaggcgtccg gggtcggcct gctggcgatg aagaacgaga tcgccgatct
    14821 gcgcggcgca cccgccgccg tgcagttgaa ggcggacatc aagccgggtc cgggcgtcgg
    14881 catcggcagc ggcccgggcg tgggcgcggg aaccgatccg ggcgtgccgg tacgcgcgct
    14941 gatcacatcg aatgccacca acggcagcgg cggcatcaac accgctgcgt acagccgtga
    15001 caccggcggc ggcgggctcg caggccgctc gaccacgctg gtcgaaggcg tggccggcgg
    15061 cggcggtggc ggcggttacg ggggcggcgg cggtgtgcgc aacggtggcg gccgcggatc
    15121 cggcaccggc gcgggcggcg tgctgcagcg aagcggcagc ggcaaggcgt cgcgttcgat
    15181 cgaggagatc aagctcgtct tcgagcgcaa caagggtgcg atctacgcga tctataaccg
    15241 cgcgctgcgc gaggacccgg cgttgcaggg caaggtcgtg ctggagctga cgatcgcgcc
    15301 gacgggaaac atcacgaacc tgcgcatcct ttcgagcgaa ctgaaggctc aggaactgga
    15361 gcagaagctg ctcgcgcgca ttcgccagtt cgacttcggc gcgaaggacg tcgaggtgat
    15421 ggtggtgtcg tggccggtgg atttcctgcc gtcgtgagcg gacgggcctg actcagaccg
    15481 caaccgccag tcgcgtcgcc tgttcgatcg ctcttgcggc atcgagttcg gcggcgacat
    15541 cggcgccgcc gatcaggtgg gctccgatgc cgcgcgcctt caggtcttcg tagagatcgc
    15601 gctcgctttc ctgccccgcg cacagcacga cgtgatcgac gtcgagcacg cgcgtctctc
    15661 cgccgacggc atagtgcagg ccggcatcgt cgatggccag gtacgtcgcg ccggcaacca
    15721 tctgcacgcc ggcctcctgc agtctcgcct tcagaatcca cccggtcgtc ttccccaggt
    15781 tcgcgccgag cttgtcgatc ttgcgctgga acatgtgcac ctcgcgcttc ggcgcgggcg
    15841 actttgcgcg cgtcgcaccg tgcaggccgc cgctgcgcgc cgacggatcg acgttccacg
    15901 cgtccatgaa gcggtcgggc aggcgtgatt catcggcgtc gccgagcagg aactcggcga
    15961 cgtcgaagcc gatgccgcca gcgccgatga tcgccacgcg ccggccgacc tgcttgcggc
    16021 gcgtcagcac gtcgacgtac gaaatcacct tcgggtgatc gatgccggcg aacttcggca
    16081 cgcgcggctt cacgccggtg gcgagcacga tggcgtcgaa cgcctcgcgc gcgagcgaat
    16141 cggcatcgac ccgcgttccg agtcggatcg gcacgccgag ccgctcgagg cgcacgcgga
    16201 agtagcgcag catctcgttg aactcggact tgccgggcac cacccgcgcc atgttcaact
    16261 ggccgcccag ttcgcttgct gcttcgtaca ggtgcaccgc gtggccgcgc tcggccgcgt
    16321 tgatcgagaa cgccatgccg gccggcccgg cgccgacgac cgcgatgcgc ttgcgccgcg
    16381 ccgcgggcgc ctcactgaac tcgatctcgc gcccggcacg cggattgacg agacacgttg
    16441 ccgcgcgctc ggtgaagatg cggtcgaggc acgactggtt gcaggcgatg caggtgttga
    16501 tctcgtcggc gcggctttcg cgcaccttgc gcgcgaagtc cgggtcggcg agcagcgggc
    16561 gcgccatcga cacgaggtcg gccacaccct cggcgagcag tgactccgcg acgtccgggt
    16621 cgttgatgcg attggacgcg atcaccggaa tcgtcaccgc ctgcttcacg cgccggatcg
    16681 cgaaggacca cgcggcacgc ggcacgctgg ccgcgattgt cggcacgggg gactcgtgcc
    16741 agccgatgcc ggtgttcagg atgttggtgc ccacgtcctg cacgcgccgc gccagttcga
    16801 ccgtctcggc cgccggaacg ccgccttcga ccagatcgat cgacgagatg cggtagatca
    16861 gcaggaagtc ggcgccggcc gccttgcgca ccgccttgac gatctcgacc ggcaaccgcg
    16921 cgcggttgtc gaacgagccg ccgaacgcgt cggtgcgacg gttggtcgcc ggcgcggtga
    16981 actcgttgat caggtagccc tcggaagcca tgatctccac gccgtcgtaa ccggcgcggc
    17041 gcgcgagcgc cgcggtgcgc gcgaagtcgc cgatcgtgcg ccacacctcg tcggtcgtga
    17101 gcgcccgcgg cgtgaaacgg ttgattcgtg cgcgaatcgc cgacgggccg acgcattccg
    17161 gcagcttcgc gtagcggcca gtgtgcagga tctgcagggc cacgcggccg ccggcgcgat
    17221 gaaccgcctc gcataccgcg cgatgcaggt cgagctgcga ttcgtcattc agcagcgggc
    17281 cgtccggttc gatgcggccc tccgggcttg gcgcgtaccc gccggtcagc agcagaccgg
    17341 tctcgccgcg cgcccgagca gcgtagaacg cagcgaggcg ttcggcaggc cgctcgaggg
    17401 tctccagccg cgtgtgcatc gcgcccatca cgatgcggtt cccgagcgtc aggaagccga
    17461 agtcgagcgg cgacaacaga tgcgggtggc tactcatggt catctcgtgc ttgcgcgatg
    17521 tggttggcgg tgagcacctg catgcctccg gcgcgctcgc gtgcgggcgg cgcgcgagcc
    17581 gatgtgtgac ggcgtt
//
LOCUS       DBNHBY010000323         2396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1102736, whole genome shotgun sequence.
ACCESSION   DBNHBY010000323 DBNHBY010000000
VERSION     DBNHBY010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1102736"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1202)
                     /gene="hypD"
                     /locus_tag="AC16HB_07925"
     CDS             complement(48..1202)
                     /gene="hypD"
                     /locus_tag="AC16HB_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR5606758.1"
                     /translation="MKYIDEFRDGAVARRIAAQIAAEVEPQRRYHFMEFCGGHTHAIS
                     RYGVADLLPAAVRMIHGPGCPVCVLPIGRIDLAIRLALETGGEQRAIVATYGDCLRVP
                     ASNGLSLLKAKARGGDVRMIYSAADALELARRHPQREVVFFAIGFETTTPPTALAIRQ
                     AAAEGLANFSVVCNHVLTPAAITHILESPEVREFGTVPIDGFIGPAHVSTVIGSAPYE
                     HFAEEYRKPVVIAGFEPLDVMQAILMLVRQVNDGRAGVENEFTRAVTRDGNVKAQQLV
                     SEVFELRRSFEWRGLGEVPYSALAIRAPFAAFDAERKFALETRKVADNKACECGAILR
                     GVKHPRDCKIFGTVCTPDNPVGSCMVSSEGACAAHYSYGRFKDVPIAAVA"
     gene            complement(1199..1420)
                     /locus_tag="AC16HB_07930"
     CDS             complement(1199..1420)
                     /locus_tag="AC16HB_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR5606759.1"
                     /translation="MCLAIPVRVVEKRSDDLAVVDLSGIRKEISLALIDDVQVGDYVI
                     LHVGYALAKLDRDEAERTLALFAEAGLGA"
     gene            complement(1423..>2396)
                     /locus_tag="AC16HB_07935"
     CDS             complement(1423..>2396)
                     /locus_tag="AC16HB_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
                     /protein_id="HIR5606760.1"
                     /translation="LENAASCIALEEAVAHLQDVLAVRPQALAHDLHPDFFSTRLAAQ
                     LAAELGVPAIGVQHHHAHVAAVCAEHGVAAPVLGLALDGVGHGDDGDAWGGELLLVDG
                     AACRRLGHLRALPLPGGDRAAREPWRMAAALLHELGRNDEIARRFAHQPAAPVVADML
                     ARDLHCPRTTSAGRWFDAAAALLGVREVMNFEGQAAMLLEGLASRRGVVAPASGGYRI
                     DEGTLDLLPLAARLLDERDAEFGAALFHSTLAAALAEWVAAASRATDLTTVALGGGCF
                     LNAVLSRQLARLLKARGLDVLEARQAPPNDGGIALGQAWVALQKMEP"
BASE COUNT          371 a          876 c          800 g          349 t
ORIGIN      
        1 gccgcacgta gccggatttg acgggcctgc tcatgcgtcc gtccatctca cgccaccgcg
       61 gcaatcggca cgtccttgaa gcggccgtag gaataatgcg ccgcgcacgc gccttcggag
      121 ctcaccatgc acgaaccgac cgggttgtcc ggcgtgcaga cggtgccgaa aatcttgcag
      181 tcccgcgggt gcttgacacc gcgcaggatc gcgccgcatt cgcacgcctt gttgtcggcg
      241 accttcctgg tttcgagcgc gaatttgcgt tcggcatcga aggcggcaaa cggcgcgcgg
      301 atcgccagcg ccgaatacgg cacctcgccc agcccgcgcc attcgaagct gcggcgcaac
      361 tcgaagactt cgctgaccaa ttgctgcgcc ttgacgttgc cgtcgcgcgt gacggcgcgc
      421 gtgaattcgt tctcgacgcc tgcgcggccg tcgttgacct gccgcaccag catcaggatc
      481 gcctgcatga cgtcgagcgg ctcgaagccg gcgatcacga ccggtttgcg gtactcctcc
      541 gcgaaatgct cgtagggcgc actcccgatc acggtgctca cgtgcgccgg gccgatgaag
      601 ccatcgatcg gcaccgtgcc gaactctcgc acctcgggcg attcaaggat gtgcgtgatc
      661 gccgccggcg tcagcacgtg gttgcagacg acgctgaagt tggcgagccc ttcggcggcc
      721 gcctgcctga tcgccagcgc ggtcggcggc gtggtggtct cgaagccgat cgcgaagaac
      781 accacttcgc gctgcggatg gcgacgcgca agctcgagcg cgtcggcagc cgagtagatc
      841 atgcgcacgt cgccgccacg cgccttcgcc ttcagcagcg acaggccgtt cgaagccggc
      901 acgcgcaggc agtcgccata agtggcgacg atcgcccgct gctcgccccc ggtttccagt
      961 gcgagcctga tcgccaggtc gatgcgaccg atcggcagca cgcacaccgg acagccgggc
     1021 ccgtggatca tccgcaccgc ggccggcagc aggtcggcca caccgtagcg cgagatcgcg
     1081 tgcgtgtgcc cgccgcagaa ctccatgaag tgatagcgcc gctgcggctc gacctcggcg
     1141 gcgatctgcg cggcgatcct gcgcgcgacc gcaccgtcgc gaaactcgtc gatgtacttc
     1201 atgcgcccag gcccgcttcg gcaaacagcg ccagcgtgcg ctcggcctcg tcgcgatcga
     1261 gcttggcgag cgcgtagccg acatgcagga tcacgtagtc gccgacctgc acatcgtcga
     1321 tcagcgcgag cgagatctcc ttgcggatgc ccgacaggtc gacgacggca agatcgtcgc
     1381 tcctcttctc gacaacgcgc acggggatgg caaggcacat cgtcagggct ccatcttctg
     1441 cagcgcgacc cacgcctggc cgagcgcgat gccgccgtcg ttgggcggcg cctgccgcgc
     1501 ttcgagcacg tcgagcccgc gcgctttcag caatcgcgcc agctgccgcg acagcacggc
     1561 gttcaggaag cagccgccgc cgagcgcgac cgtcgtgagg tcggtcgcgc gcgatgcggc
     1621 cgcgacccat tcggcgagcg ccgcggctag cgtgctgtgg aacagcgccg cgccgaattc
     1681 ggcatcgcgc tcgtcgagca ggcgcgccgc gagcggcagc aggtcgagcg tcccctcgtc
     1741 gatgcggtag ccgccgctcg ccggcgcgac cacgccgcgc cggctggcca ggccttcgag
     1801 cagcatcgcc gcctggcctt cgaaattcat cacttcgcgc acgccaagca gcgcggcggc
     1861 cgcatcgaac cagcgaccgg cgctggtcgt ccgcgggcaa tgcaggtcgc gcgccagcat
     1921 gtcggccacg acgggcgcgg cgggctggtg cgcgaaacgc cgcgcgatct cgtcgttgcg
     1981 cccgagttcg tgcaacagcg ccgccgccat ccgccagggc tcgcgcgcag cgcgatcgcc
     2041 gccgggcagc ggcagcgcgc gcaggtggcc gagccgcctg catgcggcgc catcgaccag
     2101 cagcagttcg ccgccccacg catcgccatc gtcaccgtgc ccgacgccgt cgagcgccag
     2161 cccgagcacc ggcgccgcga cgccatgctc ggcacacacc gccgcgacgt gcgcgtgatg
     2221 gtgctgcacg ccgatcgcag gcacgccgag ttcggccgcc agttgcgcag cgagccgcgt
     2281 gctgaagaaa tccggatgca gatcgtgcgc gagcgcctgc gggcgcactg cgagcacgtc
     2341 ctgcaggtgc gcgaccgcct cttccagcgc gatgcagctc gcggcgttct ccaggt
//
LOCUS       DBNHBY010000324         1991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1033811, whole genome shotgun sequence.
ACCESSION   DBNHBY010000324 DBNHBY010000000
VERSION     DBNHBY010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1033811"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>1991
                     /locus_tag="AC16HB_07940"
     CDS             34..>1991
                     /locus_tag="AC16HB_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5606761.1"
                     /translation="MSPVDGTERFGAVQAVPDYPLEVIVTRDVARALAPWREMAWGTG
                     LRTLAMALLAASLLWLLMRKIERLAAVHDKLDTSRERFALAVAGSDDGIWDWDQRTDV
                     VFASARARELYGLPPGPETQTRAEWFRQVEIHPEDAPQRREAIEAHMAGKTPHYEGEY
                     RVRHPDGSWRWVRVRGLCIRDAAGQPSRMAGSVTDIDARRRAEEALRQSEDRYAIAMT
                     GSDEAHWVWDMATDELFASAKLTEVFDLPPGFNAKTRNDLLAQMPFHPGDLERLNKAV
                     DDHVAGRAPRFDIEYRIITRAGDVRWLHARGRCFRDADGTPVRMAGSTVDVTERKRAE
                     QALRESEERFSLAVAGANDGILDWDIVNDRLFSSPRALQILGIDSDVTVRSQAEWSAL
                     VMPRFHPDDVERVANDLATRPDFHDGEYRVRAADGRYVWVRFRGMQVRDAAGRAIRWA
                     GSVSDIDAGKRTEEALRESERRYQLAVAGVNQGVWDWDLASDRVFMSARAQLLFGQDP
                     GEPLRPRREWLAHWTYHPDDRLRVRQAVSDYLRGVTRTFEVEYRMLHPSGQWRWYRDR
                     GVALRDDSGRPYRMAGSIEDITDRKHAEAERDRLEGQLRQAQKLEAMGTLAGGIAHDF
                     NNILAAILGYGEMAQKDAPEGSSLRRHL"
BASE COUNT          300 a          670 c          752 g          269 t
ORIGIN      
        1 cgcgagtccg gccggcccgg gccgatgcgg tcggtgagcc cggtcgacgg cacggagcgc
       61 ttcggcgccg tgcaagcggt gccggactac ccgctcgaag tgatcgttac gcgcgacgtg
      121 gcgcgggcgc tggcgccatg gcgcgagatg gcatggggca cgggcctgcg caccctggcg
      181 atggcgttgc tggcggcgtc gctgctgtgg ctgctgatgc gcaagatcga gcggctggcc
      241 gcggtgcacg acaagctcga cacgtcgcgc gaacgtttcg cgctggcggt ggccggctcc
      301 gacgacggca tctgggactg ggaccagcgc accgacgtgg tgttcgcgtc ggcgcgcgcg
      361 cgcgaacttt acggcctgcc gccgggaccc gagacgcaga cgcgcgccga atggttccgg
      421 caggtcgaga tccatccgga ggacgcgcca caacgacgcg aggcgatcga ggcgcacatg
      481 gccggaaaga cgccgcacta cgaaggcgag taccgcgtgc gtcaccccga cggcagctgg
      541 cgctgggtgc gcgtgcgtgg actgtgcatc cgcgacgcgg ccgggcagcc ctcgcgtatg
      601 gcggggtcgg tcaccgacat cgacgcgcgc cggcgcgccg aggaggcgct gcggcagtcg
      661 gaggaccgct acgcgatcgc gatgacgggc tcggacgagg cgcactgggt gtgggacatg
      721 gcgaccgacg agctgttcgc gtcggcgaag ctcaccgagg tcttcgatct gccgccggga
      781 ttcaacgcca agacccgcaa cgatctgctg gcgcagatgc catttcatcc cggcgatctt
      841 gagcggctga acaaggcggt cgacgaccat gtcgccggcc gggcgccgcg gttcgacatc
      901 gagtaccgga tcatcacgcg cgccggcgac gtgcgctggc tgcatgcgcg cggccgctgc
      961 ttccgcgatg ccgacggcac gcccgtgcgc atggccggct cgaccgtcga cgtcaccgag
     1021 cgcaaacgcg ccgagcaggc gctgcgcgag agcgaggagc gcttctcgct cgccgtggcc
     1081 ggcgccaacg acggcattct cgactgggac atcgtcaacg accgcctgtt cagttcgccg
     1141 cgcgcgctgc agatcctcgg catcgactcg gatgtcacgg tgcgctcgca ggcggagtgg
     1201 tcggcgctgg tgatgccgcg cttccacccc gatgacgtcg agcgcgtcgc caacgatctg
     1261 gcgacccggc ccgatttcca cgacggcgag taccgcgtgc gcgccgccga cggcaggtac
     1321 gtatgggtgc gcttccgcgg catgcaggtg cgcgacgcgg cgggccgcgc gatccgctgg
     1381 gccggatcgg tcagcgacat cgacgcgggc aagcgcaccg aggaggcgct gcgcgagtcg
     1441 gagcggcgct atcagcttgc cgtggccggc gtgaatcagg gcgtgtggga ctgggatctc
     1501 gccagcgacc gggtgttcat gtcggcgcgc gcacagctgc tgttcgggca ggatcccggc
     1561 gagccgctgc gaccgcggcg cgaatggctg gcgcactgga cctaccatcc ggacgaccgg
     1621 ctgcgcgtgc gccaggcggt gtccgactac ctgcgcggcg tcacacgcac gttcgaggtc
     1681 gaataccgga tgctgcatcc gtccgggcag tggcgctggt accgcgaccg cggcgtggcg
     1741 ctgcgcgacg acagtgggcg gccgtaccgg atggccggct cgatcgagga catcaccgac
     1801 cgcaagcacg ccgaggccga gcgcgaccgg ctcgagggtc agctgcggca ggcgcagaag
     1861 ctcgaggcga tgggcacgct cgccggtggc atcgcgcacg acttcaacaa catcctggcg
     1921 gcgatcctcg gctacggcga gatggcgcag aaggatgcgc ccgaagggag cagcctgcgg
     1981 cgccacctcg a
//
LOCUS       DBNHBY010000325         3725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_222545, whole genome shotgun sequence.
ACCESSION   DBNHBY010000325 DBNHBY010000000
VERSION     DBNHBY010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_222545"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..653)
                     /locus_tag="AC16HB_07945"
     CDS             complement(216..653)
                     /locus_tag="AC16HB_07945"
                     /inference="COORDINATES: protein motif:HMM:NF052504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sll1863 family stress response protein"
                     /protein_id="HIR5606762.1"
                     /translation="MNRLTMAAVIVCAALGAVGCSKQERTASSGVTAQDVKREATEAV
                     DTTTQYAQQEKAEFIRNAEHELAEMKANLDELNSRAQAASGESKARLETQVVALQSKW
                     RAAESKLNEAKAAAAPTWADAKASFAQSMQELKQTLEDSKRQG"
     gene            828..1586
                     /locus_tag="AC16HB_07950"
     CDS             828..1586
                     /locus_tag="AC16HB_07950"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5606763.1"
                     /translation="MRENRLRALWREDRAAVNGWLAIPDSFSAETMAHAGWDSLTIDL
                     QHGIVDYQAMVPMLQAISTTATVPLVRVPWLEPGILMKTLDAGAYGVICPMVNTREDA
                     QRLVAYTHYAPKGTRSFGPMRGFLYGGADYPQHADATIVRFAMIETRQALDNLDAILS
                     VEGLDAIYIGPSDLSLSLGCRPDMDNPEPPVAQAIDHILARAKAHGVVAGIHNMGADA
                     ARARIAKGFRFVTIGSDARLLAAGAQQVVAQMRA"
     gene            complement(1589..2011)
                     /locus_tag="AC16HB_07955"
     CDS             complement(1589..2011)
                     /locus_tag="AC16HB_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606764.1"
                     /translation="MTRFPTGSRTCIAAIATAACLIFPAHGFAADATGARTRLVRVLP
                     DHYLAGGKRFHDLDALEAWVTASGATALRLESCTSAANKALGAATARFRHMYLDLRVP
                     ASGQVACRDARIQQAGIARLEIAPAADDVRRYWESVSP"
     gene            complement(2172..2987)
                     /locus_tag="AC16HB_07960"
     CDS             complement(2172..2987)
                     /locus_tag="AC16HB_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5606765.1"
                     /translation="MSASDIRATVVLLHSSASSARQWDALLDLLRPRFRVHAIDLHGH
                     GTRSEWPGDTPFALADDAALVESLLADAGSVHLVGHSYGAAVALKLATLHPQGVRSVV
                     AYEPVLFRWLIDDVARHHHPVQDVLGVAVAMRERVACGRHDEAARRFIDFWSGTGAWD
                     ALPAERQRSIARRTPVLLQQFDALFREPFPRAQLARCRAPLLFLSGARTVAVARRIAD
                     LLRGALPAAEHETLPQAGHLGPITHAADVNRRIVTFLAAHAGDANFEPLRAAA"
     gene            complement(3017..3268)
                     /locus_tag="AC16HB_07965"
     CDS             complement(3017..3268)
                     /locus_tag="AC16HB_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiS-like domain-containing protein"
                     /protein_id="HIR5606766.1"
                     /translation="MTRASHDARPALLDCIGIALEQLAASLRRLSAELERRQRIRDTY
                     LALRELDARTLRDLGFHRSEIRSIAHDVAGTAAAVHARG"
     gene            complement(3362..>3725)
                     /locus_tag="AC16HB_07970"
     CDS             complement(3362..>3725)
                     /locus_tag="AC16HB_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606767.1"
                     /translation="RVADAIAGYSAVIVHFDQRQMQVVSLVRALRSRADAYRTQGNID
                     AALADAQRARQISRTLQGGRAHSGLTGLALNVLARVQQARGQSAAARAAAAEALPHLT
                     EALGADHAETLAARRAAS"
BASE COUNT          563 a         1331 c         1274 g          557 t
ORIGIN      
        1 accggacatc tactgaacgt cgccccggcg caggccgagg cccgatccgt gcgcacccaa
       61 agaccagacc ccggcctgcc gggcgaccaa accctgccga agcccgaaac acgaaacaga
      121 ctcaagagcg caagggcctg aatccgacca cggtgttccg ggactacggt catccgcagc
      181 gcaaggcggt cgaggagtgc ccgccgcggg cgccttcagc cctgacgctt gctgtcctcc
      241 agcgtctgct taagttcctg catcgactgc gcgaagctcg ccttcgcatc ggcccacgtc
      301 ggtgcggccg cggccttcgc ctcgttcagc ttcgattcgg ccgcgcgcca cttgctctgc
      361 agtgccacga cctgcgtctc gagcctggcc ttgctctcgc cgctggcggc ctgcgcgcgg
      421 ctgttcagct cgtccaggtt cgccttcatc tccgccagct cgtgctcggc attgcgtatg
      481 aactccgctt tctcctgctg cgcgtattgc gtggtggtgt cgaccgcttc cgtcgcctcg
      541 cgcttcacgt cctgcgcggt cacgccggaa ctcgcggttc gctcctgctt gctgcagccc
      601 acggcgccca gtgcggcgca gacgatcacc gccgccatcg tcagtcgatt catgtgccct
      661 ccggtcgttg cccgatgact gcacggtacc gacgcgccga tagcgcggct atcggccgac
      721 ggcggctcct cgcgtaggaa tccgctgaca gcgcgatggc acagccgcgc cgtcgcgcgc
      781 tacgctcccg cgtcgaccac gatcccgacg aagacaccgg agtcaccatg agagagaacc
      841 gcctgcgcgc tctgtggcgc gaagaccggg ccgccgtcaa cggctggctc gcgatccccg
      901 attcgttcag cgccgaaacg atggcgcacg ccggctggga ctctctgacg atcgacctgc
      961 agcacggcat cgtcgactac caggcgatgg tgccgatgct gcaggcaatc tcgaccacgg
     1021 cgaccgtgcc gctcgtacgc gtaccgtggc tggagccggg gatcctgatg aagacgctcg
     1081 acgccggcgc ctatggcgtg atctgcccga tggtcaacac acgcgaggac gcgcagagac
     1141 tcgtcgcgta cacgcactac gcgccgaaag gtacccgcag cttcggcccg atgcgcggat
     1201 tcctgtacgg cggcgccgac tacccgcagc acgccgatgc gacgatcgtc cgcttcgcga
     1261 tgatcgagac gcggcaggcg ctcgacaacc tggacgcgat cctgtcggtc gaggggctcg
     1321 atgcgatcta catcgggccg tcggaccttt cgctgtcgct cggctgcagg cccgacatgg
     1381 ataacccgga accaccggtg gcgcaggcga tcgaccacat cctcgcgcgc gccaaggcgc
     1441 acggagtggt cgccggcatc cacaacatgg gcgccgacgc ggcgcgcgcg cgtatcgcga
     1501 agggcttccg cttcgtgacg atcggctctg acgcgcggct gctggccgcg ggcgcgcagc
     1561 aggtcgtcgc gcagatgcgc gcctgatgtt acggcgaaac gctctcccag tagcgcctga
     1621 cgtcgtccgc cgcgggcgcg atttccagac gcgcaatgcc ggcctgctgg atgcgcgcgt
     1681 cgcggcacgc gacctggccg ctcgccggca cgcgcagatc gagatacatg tgacggaagc
     1741 gcgccgtggc cgcgcccagc gccttgtttg ccgcggacgt gcagctttcc aggcgcagcg
     1801 cggtcgcgcc gctcgccgtc acccaggctt ccagcgcatc gaggtcgtgg aatcgcttgc
     1861 cgcctgcgag gtagtggtcg ggaagaaccc gcacgagccg cgtgcgcgcg ccggtggcgt
     1921 cggcggcaaa gccgtgggcg ggaaatatca ggcaggccgc cgtggcgatg gcagcgatgc
     1981 aggtgcgcga accggtcggg aatcgagtca tggtgtcgtg cctccttgcg taattgattc
     2041 ggtcggggcg acgtccgcgt gttggcgatg tcggtgccgg attgttcggt cgcgtgccgc
     2101 tgcgctcaag cgaccttttc tcaccgcaag catgagttcc gggaatggaa ggtcgcgggc
     2161 cgccgactcg ctcacgccgc cgcgcgcagc ggctcgaagt tcgcatcccc ggcgtgcgcg
     2221 gcaaggaagg tcacgatccg ccggttgaca tcggccgcgt gcgtgatcgg tcccaggtgc
     2281 cccgcctgcg gaagcgtctc gtgctccgcg gcgggcagcg cgccacgcag cagatccgcg
     2341 atccggcgcg cgaccgcgac ggtgcgcgca ccgctgagga acagcagcgg tgctctgcag
     2401 cgcgccagtt gcgcgcgcgg aaagggctcg cggaacagcg cgtcgaactg ctgcagcagg
     2461 accggcgtgc ggcgcgcgat cgagcgctgc ctctcggccg gcagggcgtc ccatgcgccg
     2521 gtgccggacc agaagtcgat gaatcgccgc gcggcctcgt cgtgccggcc gcaggcgacg
     2581 cgctcgcgca tcgcgacggc cacgccgagc acgtcctgca ccgggtgatg atggcgcgca
     2641 acgtcgtcga tcagccagcg gaacagcacc ggctcgtagg cgactacgct gcgcacaccc
     2701 tgcggatgca gggtcgcaag tttcagcgcg acggcggcgc cgtaggaatg ccccaccaga
     2761 tgcacgctgc ccgcgtccgc cagcagtgat tcgacgagcg cggcgtcgtc ggccaaggca
     2821 aacggggtgt cgccgggcca ttcggagcgg gtgccgtggc cgtgcaggtc gatcgcgtgc
     2881 acgcggaaac gcgggcgcag cagatcgagc agcgcgtccc actgccgcgc cgaactggcg
     2941 ctcgaatgca gcagcacgac tgtcgcgcga atgtccgaag cggacatggc tcctccacgg
     3001 atgcgaattg gcttcgtcac ccgcgcgcgt ggacagccgc ggcagtgccg gcgacgtcgt
     3061 gcgcgatcga ccggatttcg ctgcggtgga agccgaggtc gcgcagcgtg cgtgcgtcga
     3121 gttcgcgcaa cgcgaggtag gtgtccctga tccgctgccg ccgctcgagt tcggccgaca
     3181 ggcgccgcag cgaggcggcc agttgctcca gcgcgatgcc aatgcagtcc agcagcgccg
     3241 gccgcgcgtc atgcgaggct cgagtcatgc gacgttctcc cttggttggc agggcccgac
     3301 cggcccctca ttccaagatc gaaatgccgc ggcgaaaccc gacaccccgc aacgggcggg
     3361 ctcaactcgc agcgcgccgc gccgccaagg tctccgcgtg atccgcgccg agcgcttcgg
     3421 tcaggtgcgg caaggcctcc gccgccgcgg cacgcgcggc ggcgctctgg ccgcgcgcct
     3481 gctgcacgcg cgccagcacg ttcaacgcca gccccgtcag accggagtgt gcgcggccgc
     3541 cctgcagcgt gcgcgagatc tgccgcgcgc gctgcgcgtc ggccagcgcg gcgtcgatgt
     3601 tcccctgagt ccggtacgca tcggcacgcg accgcagcgc gcgcaccagc gacacgacct
     3661 gcatctgcct ctggtcgaag tgcacgatca ccgcggagta cccggcgatg gcgtcggcga
     3721 cgcga
//
LOCUS       DBNHBY010000326         4812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1575133, whole genome shotgun sequence.
ACCESSION   DBNHBY010000326 DBNHBY010000000
VERSION     DBNHBY010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1575133"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="AC16HB_07975"
     CDS             complement(<1..940)
                     /locus_tag="AC16HB_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5606768.1"
                     /translation="MPDSLPPNLRNIGRYALLRELRRDRVSVSYFAMDPVLHREVIIK
                     AVQLPLPSDGAPGRDAQISPVEQAFVRQAQAAGKLHHPHIVTVFEAGRMRSIGYLAIE
                     RVPGRRLHELIASGWRPEFVHAASIAARIADAIEYAHLQNVAHGHLGPEQVILHEADG
                     APKVEGFGGWVDGGESGEDALARTERVLPYFDEVRDDVRRRDVRAVAALVYMMLTGRS
                     PDTPATRLNGPAPLRSLRLDTPPELADLIDAALAADPQHARRSAADLRDALTAFIWNE
                     RTHNVAPATIGIPLAAPPAPAETEAPPPAPASLPRTA"
     gene            complement(1064..2455)
                     /locus_tag="AC16HB_07980"
     CDS             complement(1064..2455)
                     /locus_tag="AC16HB_07980"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5606769.1"
                     /translation="MNVRAPVTISTEATPSLEAAPSTIGRFNVLGEIGRGSNGVVYAA
                     RDPVLGREVAIKAIPLTSENEFRQSVEENFIKESQAAAGLNHPHIVTVFDAGKTDSIA
                     YMAMERLHGRDLHEQLAVGPPLNFRQAAALIARVADALHYAHKRGLIHRDIKPSNIFL
                     TRDMKPKILDFGVALAARADLDPSDRRQLVGTPNYMSPEQALGKPLDARSDVFSAGTI
                     LYELVGGKRAFDGKSVEDTIAAVIKCDPTPIATLRPDVPAELAQIIRRAMAKSPNDRY
                     QTAGDLRNDLSAFAGQREFANDPLRPQTRTAYMVERSKWIAGTVTGVVIGVALGAWYL
                     WPKETPAPPTAPPTAPAASVKPAAEPAPSAAAPNPDASAPPVARAPAERKPAPTPTPA
                     APPADGTVTLAISPWGEVFVNGTSRGVAPPLNHLTLAPGVYSIEIRNTAAPAYSARVE
                     VRPGQTVTLQHRF"
     gene            2593..3489
                     /locus_tag="AC16HB_07985"
     CDS             2593..3489
                     /locus_tag="AC16HB_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5606770.1"
                     /translation="MSASASGFGTGLLLAAAGSVLFSGKAIVIKLAYRYGVDAETLLA
                     LRMLLAVPFFVIVLWWTSRGTRRLTAREHAKLVVLGLLGYYAASYADFLGLQHISAGL
                     ERLILFLNPTIVLILSVLFLARRIERRDLAALALAYGGIVLVFWHDAHGGGNVALGAG
                     LVFFSAVAYAVYLVLAGELVQRVGALRLTAYAMCVSSAAVCAQFAALRPLANLAQPVP
                     VLWLSLINAVACTVVPVFATMLAVARVGAGHASMMGMIGPVATIVLAFLFLGEAITGW
                     QLAGTALVLAGVFVLSRKERAR"
     gene            3486..4463
                     /locus_tag="AC16HB_07990"
     CDS             3486..4463
                     /locus_tag="AC16HB_07990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR5606771.1"
                     /translation="MKARAIRVEAHGGPEVLKLVEVEVGDPGPDEVRVRNHAIGLNFI
                     DIYYRTGLYPSPPPHGLGFEGAGIVEAVGANVKFLKEGDRVAYPQGPLGAYADVRVMP
                     AATVVRLPKKIGFDEGAAVMLKGLTVQYLFRQTYRLQGNETILFHAAAGGVGLIACQW
                     AKALGVKLIGTVSSEEKAALARKQGAWQTINYAKENFVERVKELTGGARVPVVYDGVG
                     KDTWEGSLDCLQPRGLMVSFGNASGAVTGVSIGVLAAKGSLFVTRPTLMTHITPRRKL
                     EEAAEELFDLMLKGKVKVEIDQRYPLAVAARAHEDLAARKTTGSTVLVP"
     gene            complement(4471..>4812)
                     /locus_tag="AC16HB_07995"
     CDS             complement(4471..>4812)
                     /locus_tag="AC16HB_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR5606772.1"
                     /translation="RGFKVMHLFRKDCGAAPTAELRVALKDGKALCVYGGKVETAKLE
                     SGADYVMSADTARWVEMGRGDYGPMRAMMFGRLEFAGPKMEAMGNMGPFENFLRLVGK
                     VPGDTKGCPGT"
BASE COUNT          684 a         1603 c         1732 g          793 t
ORIGIN      
        1 gcgccgtgcg tggcagcgat gccggagcgg gtggcggcgc ctccgtctcg gcgggcgccg
       61 gaggcgcggc gagcggaatg ccgatcgtgg ccggcgcgac gttatgcgtg cgttcgttcc
      121 agatgaatgc ggtcagggcg tcgcgcaggt cggcggcgct gcggcgcgcg tgctgcgggt
      181 cggcggcgag cgcggcgtcg atcaaatctg ccagttccgg cggtgtgtcg agccgcagcg
      241 aacgcagcgg cgccggcccg ttcaggcgcg tggcgggtgt gtcgggcgaa cggccggtca
      301 gcatcatgta gacaagtgcg gcgacagcgc gcacatcgcg ccgccgcacg tcatcacgca
      361 cctcgtcgaa gtacggcagc acgcgctcgg tgcgtgccag cgcgtcctcg ccggactcgc
      421 cgccgtcgac ccagccgccg aagccctcga ccttcggcgc gccgtccgcc tcgtgcagga
      481 tcacctgctc ggggccgagg tggccgtgcg cgacgttctg caggtgcgcg tactcgatgg
      541 cgtcggcaat gcgcgcggca atgctcgcgg catgcacgaa ctccggccgc cagccgcttg
      601 cgatcagctc gtgcagacgc cgcccgggca cgcgctcgat cgccagatag ccgatgctgc
      661 gcatccggcc cgcctcgaac acggtcacga tgtgcgggtg gtgcagcttg ccggcggcct
      721 gcgcctggcg cacgaaagcc tgctcgaccg gcgagatctg cgcgtcgcgt cccggcgcgc
      781 cgtcgctcgg cagcggcagc tgtaccgcct tgatgatcac ctcgcggtgc aggaccggat
      841 ccatcgcgaa gtacgacacg ctgacgcggt cgcgccgcag ctcgcgcagc agcgcgtagc
      901 ggccgatgtt tctcagattg gggggcagtg aatcgggcat ggctttgcgt ttgcctcggc
      961 gagggtagcg taggcgtgcc ggcgtctcaa tccggccgaa cgaatggggc gtggtcccct
     1021 aaacgggcgt ggcttcggtc cgtcgcgccg ccgcgcgttg gcgtcagaag cgatgctgca
     1081 gcgtgaccgt ctggccgggc ctgacttcga cgcgcgccga gtaggccggc gcagcagtat
     1141 tgcgaatctc gatcgaatac acacccggcg cgagcgtgag atggttcagc ggcggcgcga
     1201 cgccgcgcga ggtgccattg acgaagacct cgccccacgg cgagatcgcg agcgtgaccg
     1261 taccgtcggc cggcggcgcc gcaggtgtcg gcgtcggcgc gggcttgcgc tcggctggcg
     1321 cgcgcgcgac gggtggcgcg ctcgcatccg ggttcggcgc tgcggcgctc ggagccggct
     1381 cggccgcggg cttcaccgaa gcggcaggcg cggtcggcgg cgcggtcggc ggcgccggcg
     1441 tttccttcgg ccacagatac cacgcgccga gcgcgacacc gatcaccacg ccagtgacgg
     1501 tgccggcgat ccacttgctg cgctcgacca tgtatgcggt gcgcgtctgc ggccgcagcg
     1561 gatcgttcgc gaattcacgc tggcccgcaa aggcggaaag atcgttgcgc aggtcgccgg
     1621 ccgtctgata gcggtcgttg ggcgacttgg ccatcgcgcg gcggatgatc tgcgcgagtt
     1681 ccgccggcac gtcgggacgc aacgtggcga tcggcgtcgg atcgcacttg atcacggcgg
     1741 cgatcgtgtc ctcgacgctc ttgccgtcga aggcgcgctt gccgccgacc agctcgtaca
     1801 ggatcgtgcc ggcggaaaac acgtcgctgc gcgcatccag cggcttgccg agcgcctgtt
     1861 cgggcgacat gtagttcggc gtgccgacca gttgccggcg atcgctcgga tcgagatcgg
     1921 cgcgcgcagc cagcgcgaca ccgaagtcga ggatcttcgg cttcatgtcg cgggtcagga
     1981 agatgttgct cggcttgata tcgcgatgga tgagtccgcg tttgtgcgcg tagtgaagcg
     2041 catcggccac gcgcgcgatc agcgccgcgg cctggcggaa attcaacggc gggccgacgg
     2101 cgagttgctc atgcaggtcg cggccgtgca ggcgctccat cgccatgtac gcgatcgagt
     2161 cggtcttgcc ggcgtcgaac acggtgacga tgtgcggatg gttcaggccc gccgcggcct
     2221 gcgactcctt gatgaagttt tcctcgacgc tctgccggaa ctcgttttcc gacgtcaacg
     2281 ggatggcctt gatcgccacc tcgcgtccga gcaccggatc gcgcgccgcg tagacgacgc
     2341 cgttggagcc gcggccgatc tcgccgagga cgttgaaacg cccgatcgtc gacggcgcgg
     2401 cctccagcga cggagtcgcc tcggtggaaa tcgtcacggg cgcgcgcacg ttcatcgcta
     2461 cgattattgc accggccccg cgtgcgatcc gacgcgcaaa cagatgatct cctcgcgtcg
     2521 gccgccgttc gccgcagggc gcgtccttac aatgcgcacg tgtcggcgac cgcacaaacc
     2581 aatgtctctg ccgtgagcgc atcggcctcc ggcttcggca ctggattgct gctggcggcc
     2641 gccggcagcg tgctgttctc gggcaaggcg atcgtcatca agctcgccta ccgctacggc
     2701 gtcgacgcgg aaacgctgct cgcgctgcgc atgctgctcg cggtgccgtt cttcgtcatc
     2761 gtgctgtggt ggacctcgcg cggcacgcgc cggctgaccg cgcgcgagca cgcgaagctg
     2821 gtcgtgctgg ggctgctcgg ttactacgcg gccagttacg ccgactttct cggcctgcag
     2881 cacatcagcg cgggcctgga acgactgatc ctgttcctga acccgaccat tgtgctgatc
     2941 ctgtcggtgc tgtttctcgc gcgccgcatc gagcggcgcg acctggccgc gctcgcgctc
     3001 gcgtatggcg gcatcgtgct ggtgttctgg cacgacgcgc acggcggcgg caacgtcgcg
     3061 ctcggcgccg ggctcgtctt cttcagcgcg gtcgcgtacg cggtctatct cgtgctggcg
     3121 ggcgaactgg tccagcgcgt cggcgcgctg cggctcaccg cttacgcgat gtgcgtgtcg
     3181 tcggcggcgg tgtgcgcgca gttcgcggcg ctgcggccgc tcgcgaatct ggcgcagcca
     3241 gtgccggtgc tgtggctgtc gctgatcaac gcggtggcct gcaccgtcgt gccggtgttc
     3301 gccacgatgc tggcggtcgc gcgcgtcggc gccggtcacg catcgatgat gggcatgatc
     3361 ggcccggttg cgactatcgt gctcgcgttc ctgttcctcg gcgaggccat cacgggctgg
     3421 cagctggccg gcacggcgct tgtcctcgcg ggcgtgttcg tattgtcacg aaaggagaga
     3481 gcaagatgaa ggcaagagcg attcgagtcg aagcgcacgg cgggccggaa gtcctcaaac
     3541 tcgtcgaggt cgaggtcggc gaccccgggc ccgacgaggt gcgcgtccgc aaccacgcga
     3601 tcgggctgaa cttcatcgat atctactacc gcaccgggct gtatccgtcg ccgccgccgc
     3661 acgggctcgg cttcgagggc gccgggattg tcgaggccgt cggcgctaac gtgaagttcc
     3721 tgaaggaggg cgatcgcgtc gcctacccgc aggggccgct cggcgcgtac gccgacgtgc
     3781 gcgtgatgcc ggcggcgacc gtcgtgcggc tgccgaagaa gatcggcttc gacgagggcg
     3841 cggcggtgat gctgaagggc ctgacggtgc agtacctgtt ccggcaaacc taccgcttgc
     3901 agggcaacga gacgatcctg ttccacgccg ccgccggtgg cgtcggcctg atcgcgtgcc
     3961 agtgggcgaa ggcactcggc gtgaagctga tcggcaccgt cagctcggag gagaaggccg
     4021 ctttggccag gaagcaaggc gcgtggcaga cgatcaacta cgcaaaggag aacttcgtcg
     4081 agcgcgtaaa ggagctgacc gggggcgcga gggtgccggt cgtctacgac ggcgtgggca
     4141 aggacacatg ggaaggatcg ctcgactgcc tgcagccgcg cggcctgatg gtcagcttcg
     4201 gcaacgcgtc gggtgcggtg acgggcgtga gcatcggtgt cctggccgcc aaggggtcgc
     4261 tgttcgtcac gcggccgaca ctgatgacgc acatcacgcc gcgccggaag ctcgaggagg
     4321 ccgccgagga actcttcgac ctgatgctca aaggcaaggt caaggtcgag atcgaccagc
     4381 gctatccgct ggccgtcgcg gcgcgcgcgc acgaagacct ggccgcgcgc aagacgaccg
     4441 gctcgaccgt gctggtgccg tgaagtcgga tcaggtgccg gggcagccct tggtgtcgcc
     4501 cggcaccttg cccaccagcc gcaggaagtt ctcgaacggg cccatgttgc ccatcgcttc
     4561 catcttcggc ccggcgaact cgaggcggcc gaacatcatc gcgcgcatcg ggccgtagtc
     4621 gccgcggccc atctcgaccc agcgcgcggt gtcggcgctc atcacgtagt cggcaccgct
     4681 ttcgagcttc gcggtctcga ccttgccgcc atacacgcac agcgccttgc cgtccttcag
     4741 cgcgacgcgc agctccgcgg tcggcgccgc gccgcagtcc ttgcggaaga ggtgcatgac
     4801 cttgaagccg cg
//
LOCUS       DBNHBY010000327        13175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_493113, whole genome shotgun sequence.
ACCESSION   DBNHBY010000327 DBNHBY010000000
VERSION     DBNHBY010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_493113"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="AC16HB_08000"
     CDS             complement(<1..766)
                     /locus_tag="AC16HB_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5606773.1"
                     /translation="MMRLAALVEASARAAATGSRLAKRDALAACLRATAPDEIDIAVA
                     FLSGATRQGKLGIGYATLRELRGGAAAAEPSLMLGDVDAAFGRIAAAAGKGAARERAE
                     LLGALFGRATRTEQDFLVRLLIGELRQGALEGVMLDAIAAAANVNVGEVRRAAMFTGN
                     LGEVARAALTQGAPALARIAIQVRRPVQPMLAQPAEDIATALARLGTAALEWKLDGAR
                     VQVHKAGDEVRVFTRNLNEVTAAVPEIVETVQALPAR"
     gene            complement(763..2766)
                     /locus_tag="AC16HB_08005"
     CDS             complement(763..2766)
                     /locus_tag="AC16HB_08005"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR5606774.1"
                     /translation="MSTHGRELLTALRTFEQGKIVTAREAVQLIQDGDAIATGGFVGI
                     GFPENVAVAIEERFAESSEQDPGGIGHPRGLTLIYAAGQGDGKERGLNHLGHKGLLAR
                     VIGGHWGLVPKVQALAVANEIEGYNLPQGVITHLFRDTAAGKPGHVTRIGLGTFVDPR
                     LGGGKINAKTTEDLVSLMTIGGEEYLFYKSFHIDVGIVRGTTADVDGNITMEKEALTL
                     EALAIAMAARNSGGIVIVQVERIAERGSLNPRQVKIPGVLVDCVVVAEKPEYHHQTFA
                     EPYSPAFSGEIRVPASMVPPMPMSERKIIARRAALELKANSVVNLGIGMPEGVSGVAA
                     EEKVIDLLTLTAEPGVVGGIPASGLNFGAAVNTQAIIDQPYQFDFYDGGGLDLAFLGL
                     AQADRMGNLNVSKFGPRLAGAGGFINISQSAKSVVFVGTFTAGDLRVGVVDGKLVLHN
                     DNGPQKFVHDVEHRTFSGEYAVKRGQPVLYVTERCVFRLTPEGMELTEVAPGIDIERD
                     ILARMDFEPIIHGTPRLMEAAVFGAEPMGLRERMLAIPLASRFAYDRQQNILFINFER
                     LRVATRADVDAIEREVEAHIKPIGHRVYAIVNYDHFQLAPEVEDAYAAMVKGLVDRYY
                     DGVSRYTTSGFLRAKLGAALRTRGVAPHVYESPHEALKHVRDA"
     gene            2914..3342
                     /locus_tag="AC16HB_08010"
     CDS             2914..3342
                     /locus_tag="AC16HB_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606775.1"
                     /translation="MKARALALSLIAAAAAGAAAAQLRPPAESAPIEPLPPHALMIEA
                     ATWRPSVLLRAPADIGRSAWESATKSISLVEMPNYSLTPDRARPSYALGFASETMRNW
                     MNDLGMDAGACTAPIIRLRTRIDAGEFRGTLWVHARCSLW"
     gene            3400..4158
                     /locus_tag="AC16HB_08015"
     CDS             3400..4158
                     /locus_tag="AC16HB_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR5606776.1"
                     /translation="MDIRNNVFIVTGGASGLGGATSRMIAANGGKVVIADVQADKGEP
                     LARELGGRFVKCDVASEADGKAAVGAALALGKLRGLVNCAGIAIGEKTIGKDGPHRLD
                     AFVRVVGVNLIGSFNMIRLAADAMAKQDTTADNERGVIVNTASVAAFDGQIGQAAYSA
                     SKGGIVGMTLPIARDLARGGIRCVTIAPGIFATPMLLGMPQEVQDSLGKQVPFPSRLG
                     KPEEYAQMVKAIFENVMLNGETIRLDGAIRLQPK"
     gene            4193..5974
                     /gene="ggt"
                     /locus_tag="AC16HB_08020"
     CDS             4193..5974
                     /gene="ggt"
                     /locus_tag="AC16HB_08020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5606777.1"
                     /translation="MMTRLRATLVALAAALLAAACSTPRADRSGAPEAASGFRLGLTT
                     QTATRHIAASANPLATEAGREILRAGGSAIDAAIAMQMVLNLVEPQSSGIGGGAFLLY
                     WDGSRVQAYDGRETAPMAADENLFMAGGKPMAFYDAVVGGRSVGTPGVLRLLELAHKE
                     HGKLPWARLLEPAIRLADNGFTMSPRLNTLLAGEKYLARDAAAHAYFYSADGTPKAVG
                     AMLRNPEFAQTLRSIAAGGADAFYKGSLAADIVRAVRAHPNAGQLAENDFAAYQAKER
                     MPLCTDYKRWRICGMPPPSSGGIAVAQMLGIFTHRNISVVPPHAVDGRLEPQVDAVHL
                     FSEAGRLAFADRNLYVADPDYVPVDMLALTNPAYLADRAKLISDRSMGRAQPGVPAGY
                     RIAWASDPSPPRIATSHISVVDQYGSALAMTTTIEDQFGARMMVHGFLLNNQLTDFSF
                     VPSEDGKPVANRVQPGKRPRSSMAPTLVFDRTTNQLVATVGSPGGSQIIAYVAKTLVG
                     VLDWNLDVQQAISLPNFGSRNGPTELERGRFGNPVLEGLKARGHEVREIEMTSGLQGI
                     VRVQLPDGRPGWAGGADPRREGVALGD"
     gene            6010..6513
                     /locus_tag="AC16HB_08025"
     CDS             6010..6513
                     /locus_tag="AC16HB_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR5606778.1"
                     /translation="MTIKVGDRLPEGTLQEFIEVETAGCSLGPNTFKVQDLVKGKKIA
                     VFGLPGAYTPTCSAQHVPSYVKHYDALKAKGVDEIWCLSVNDAFVMGAWGRDQKATGK
                     VRMMADGSADYTKKLGLELDLTARGMGVRCQRFSMLVVDGVVKALNIEAPGKYEVSGA
                     ERMLTQA"
     gene            complement(6545..7378)
                     /locus_tag="AC16HB_08030"
     CDS             complement(6545..7378)
                     /locus_tag="AC16HB_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR5606779.1"
                     /translation="MNAIERFARLIERERGRPLPLLDCVAHLAQYADSGFDPDAVAGC
                     VRRWCAQLRARVAADTSPLNRLRLLNHFFFDELGFRGDHDTYDSADNSYLHRVIERRR
                     GIPISLSVLYVELGRAIGLGLDGVAFPGHFIVRLRAGDRVVFIDVFECGTTLSTAVLH
                     QRVDAFTRGRAELPLDAYLQPASDREILARWLRNLKAAHARNEDWPALLEVLNRLILL
                     LPREAVQRRDRAAVYERLECPSGAAEDLAAYLSLAPDAPDAGTMRSRLAHFQRAARRL
                     N"
     gene            complement(7375..8922)
                     /gene="murJ"
                     /locus_tag="AC16HB_08035"
     CDS             complement(7375..8922)
                     /gene="murJ"
                     /locus_tag="AC16HB_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5606780.1"
                     /translation="MNLLRAAATISSLTMLSRVTGLARDLLISRIFGASAETDAFNVA
                     FRLPNLLRRLFAEGAFQQAFVPMLADARRSRGDDGMRTLVDHVASALFWLLLAVSALG
                     VALAPALVWMIASGLAANQAAFDLATVMTRWMFPYILFMSLVALAAGVLNTMNRFAVP
                     AFTPVLLNLSFIACTLVLTPYLTRPIMALAFAVVVGGALQLGLQAAALRRLGVLPKIG
                     SLRPAFADPGVRRILQLMLPALFAVSVAQLSIIINTNIASHLASGSVTWLAFADRLME
                     FPTALLGVALGTVLLPTLSKAGAEGRADDYSRLLDWGLRLTCLIALPAALGLGLLAEG
                     LIAVLFQGGKFSAHDVAQTALALTGYAVGLLGLIAVKILAPGYYAQQNVKTPVKIAVL
                     SLLATQASNLVLVPLLAHAGLALSVGIGALVNAALLFTGLRRRGTYTPQPGWLRFSIR
                     MAIALAALAVLLWLAARRIDWIGMQSDWLVRALVLGGVVAAALGLYFGLLWLLGFRLR
                     DFSPRAR"
     gene            9064..9327
                     /gene="rpsT"
                     /locus_tag="AC16HB_08040"
     CDS             9064..9327
                     /gene="rpsT"
                     /locus_tag="AC16HB_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR5606781.1"
                     /translation="MANTKQARKRAHQNTVRNAHNTALRSRVRTAVKSVRKAIAAGDK
                     KAALEVFQQATGVIDSVADKRIVHKNTAARNKSRLAAALKAMR"
     gene            complement(9396..9743)
                     /locus_tag="AC16HB_08045"
     CDS             complement(9396..9743)
                     /locus_tag="AC16HB_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HIR5606782.1"
                     /translation="MTAEPKQQANAPREFFIHGVTIHGRTFRPSDWAERLCGVMSGFR
                     PGGVGAGRDALIGFSPYCRPTMVGGVKCVIVDERLRDIERMAFDFVMNFARDNELQVF
                     EGCSLPDPPPKSK"
     gene            9957..12008
                     /locus_tag="AC16HB_08050"
     CDS             9957..12008
                     /locus_tag="AC16HB_08050"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5606783.1"
                     /translation="MSPGPISKVLVINDEPLILRELLKGLNSAARTLDNPFGIGFIGA
                     LTAKEALHQIEEDGDIQVVIVDDKLYAVQEGARRAATRSGVRNGNGKEPSRSLQVSAL
                     KLVQKITALRPELDLYILIEKEKEDQIVDALFAEAVDGYFYREERDHRGMYRILNAQI
                     AERARTPFYDALKAYVLAAKDAWHTPGHSSGDSLRGSPWVNEFYEFMGEHVFDADLSV
                     SVKALDSLMEPTGVIAEAQQMAAKAFGARKTFFATNGTSTANKVILQTLIAPGEKMLL
                     DRNCHKSVHHGVVLSGARPIYLDSSVNRKYGVFGPVPKKTILKAIDEHPDAQLLVLTS
                     CTYDGLRYDLKPVIDAAHAKNIKVLIDEAWFSHARFHPDLRPTALESGADYATQSAHK
                     TLTAFSQAAFIHVNDPTFKEHLFRENFNMHTSTSPMYSMIASLDVGRKQAVLEGYKLL
                     ERTLMLAAELRQQINSTGVFRVLDLDDLLPDEVKGDGIRLDPTKITVDISSCGMTVDE
                     LQQELFTRFNITIEKSTFNTITLLLTIGTTRSKCSRLYDALMRIGREKRAPRRLYRTP
                     DLPPFTELVYLPRDAYYCGGELIPLLDDNDQPNAALKGRICADQIVPYPPGIPVLVPG
                     QRITENIVEYLIRYLRVQNKVELHGVVYQGYFPSVRVLMASEEQKMAPLVTRRPVRKA
                     A"
     gene            12138..>13175
                     /locus_tag="AC16HB_08055"
     CDS             12138..>13175
                     /locus_tag="AC16HB_08055"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HIR5606784.1"
                     /translation="MNTYARLPVAFTHGKGAWLYDADGTAFLDALAGIAVNGLGHGHP
                     RLVKAIADQAGKLIHTSNVYRIREQEDLADRICAITGMDEVFFANSGSEANEGALKLV
                     RFYGHKHGNPAAQTIVMEHAWHGRTLATLAATGSEKARKGFDPLPSGFIRVPYNDLAA
                     IENIAAQDKRVQAIWLEVLQGEGGIRVADRDYVRSLRRLCDERGWLLMIDEVQSGIGR
                     TGKWFGYQWAGIAPDVITIAKGLGSGVPIGAVVARGPAAGVLGPGNHGSTFGGGPLAC
                     RAGLETLAIIEDEKLMANADAQGERIRAGFKRALGDLPGVAAIRGQGLMIGIELDRPC
                     GDLARRALDARL"
BASE COUNT         2299 a         4472 c         4406 g         1998 t
ORIGIN      
        1 cgcgcgccgg gagcgcctga acggtttcga cgatttccgg caccgcggcg gtgacttcgt
       61 tcaggttgcg cgtgaagacg cgaacctcgt cgcccgcctt gtgaacctgc acgcgcgcgc
      121 cgtcgagctt ccactcgagc gcggccgtgc cgagtcgcgc gagcgccgtg gcgatgtcct
      181 cggccggctg cgcgagcatc ggctgtaccg ggcgccgcac ctggatcgca atccgggcga
      241 gcgccggagc accctgcgtc agcgcggcgc gcgcgacttc cccgagattg ccggtgaaca
      301 tcgctgcgcg ccgtacttcg cccacgttca cgttcgccgc ggcggcaatc gcgtccagca
      361 tcactccttc gagcgcgccc tggcgcagtt cgccgatcag cagccggacg aggaaatcct
      421 gctctgtgcg cgtcgcgcga ccgaacagcg cgccgagcag ttcagcgcgt tcgcgggccg
      481 cacctttgcc cgcggccgcg gcgatgcgcc cgaaggcggc gtccacatcg cccagcatca
      541 gcgaaggctc ggccgcagca gcgccaccgc gcagttcacg tagcgtcgcg tacccgatac
      601 cgagcttgcc ctggcgtgtc gcgccggaca gaaacgcgac tgcgatatcg atctcgtccg
      661 gcgcggtcgc ccgcaggcac gcggcgagtg catcgcgctt ggccagccgg ctgcccgtcg
      721 ccgccgcgcg ggcggatgct tcgaccagcg cggccaggcg catcatgcat cgcgcacgtg
      781 cttcagcgct tcgtgcggcg attcgtagac atgcggcgcg acgccgcgag tcctcagcgc
      841 agcgccgagc ttggcgcgca gaaagcccga agtcgtgtag cgcgacacgc cgtcgtagta
      901 gcgatcgacc agtcccttga ccatcgccgc gtacgcgtct tcgacttcgg gcgcgagctg
      961 gaaatggtcg tagttcacga tcgcgtagac gcggtggccg atcggcttga tgtgtgcctc
     1021 gacttcgcgc tcgatcgcgt cgacatcggc ccgtgtcgcc acacgcagcc gctcgaaatt
     1081 gatgaacaga atgttctgct gacgatcgta ggcaaagcgc gacgcgagcg ggattgccag
     1141 catgcgctcc cgcagcccca tcggttcggc gccgaagact gccgcttcca tcagccgcgg
     1201 cgtgccgtgg atgatcggct cgaagtccat cctcgccagg atgtcgcgct cgatgtcgat
     1261 gcccggtgcg acctcggtga gctccatgcc ttccggcgtg aggcggaaca cgcagcgctc
     1321 ggtgacgtac agcaccggct ggccgcgctt gaccgcatat tcgcccgaga acgtgcggtg
     1381 ctcgacgtcg tgcacgaact tctgcggccc gttgtcgttg tgcagcacca gtttgccgtc
     1441 gacgacgccg acgcgcaggt cgcccgcagt gaaggtgccg acgaacacca cgctcttcgc
     1501 cgactgcgaa atgttgatga acccgccggc gcccgcaagc cgcgggccga acttgctgac
     1561 attcaggttc cccatccggt cggcctgcgc gagcccaagg aacgccagat cgagcccgcc
     1621 gccgtcgtag aagtcgaact ggtacggctg gtcgatgatc gcctgcgtgt tgaccgcggc
     1681 gccgaagttc aaccccgacg cgggaatgcc gccgaccacg ccgggctcgg cggtcagcgt
     1741 cagcaggtcg atcaccttct cttccgccgc cacccccgac acaccctcgg gcatgccgat
     1801 gccgaggttc accacgctat tggccttcag ttccaacgcg gcgcggcgcg cgatgatctt
     1861 gcgctcgctc atcggcatcg gcggcaccat cgacgccggc acgcggatct cgcccgagaa
     1921 tgccgggctg tagggctcgg cgaaggtctg gtggtggtac tcgggttttt ccgcgactac
     1981 cacgcagtcg accagcacgc cgggaatctt cacctggcgc ggattgagcg atccgcgctc
     2041 ggcgatgcgc tcgacctgca cgatcacgat gccgccggaa ttgcgcgccg ccatcgcgat
     2101 ggcgagcgcc tccagcgtca gcgcctcttt ctccatcgtg atgttgccgt cgacgtccgc
     2161 ggtcgtaccg cgcacgatgc cgacgtcgat gtggaatgac ttgtagaaca ggtattcctc
     2221 gccgccgatc gtcatcaggg acacgagatc ctcagtcgtc ttcgcgttga tcttgccgcc
     2281 gcccaggcgc ggatcgacga aggtacccaa cccgatgcgt gtcacgtgtc cgggcttgcc
     2341 ggctgcggtg tcgcggaaca ggtgcgtgat cacgccctgc ggcaggttgt agccctcgat
     2401 ctcattggcc acggcgagcg cctgcacctt cggcaccagc ccccagtggc cgccgatcac
     2461 gcgcgcgagc aaccccttgt ggccgaggtg gttcagcccg cgctccttgc cgtcgccctg
     2521 cccggccgcg tagatcagcg tcaggccgcg cggatggccg atgccaccgg gatcctgctc
     2581 gctcgattcg gcaaagcgct cctcgatcgc caccgcgacg ttctcgggga agccgatgcc
     2641 gacgaagccg ccagtggcga tggcatcgcc gtcctggatc aactgcaccg cctcgcgcgc
     2701 ggtgacgatc ttgccctgct cgaacgtacg cagcgcggtc agcagttcgc ggccgtgggt
     2761 gctcatgaac tctcctcccc gaatcgtgag ccggtcctta cgtacgcgga attgaagcac
     2821 aaagcgctgc gctcgctcat gctttcgcat ggttgtgaga gcgccgggcc acgcggatat
     2881 tcggttcaac cgctgccaca gcgggaggtc gtcatgaagg ctcgtgcact cgcgctatcg
     2941 ctgatcgccg ctgccgctgc cggcgccgcc gcggcgcaac tgcgcccgcc ggcggagagc
     3001 gcaccgatcg aaccgctgcc gccacacgcg ctgatgatcg aggcggccac ctggcggccg
     3061 tcggtcctcc tgcgcgcacc cgcggacatc ggccggtcgg cctgggagtc ggccacgaag
     3121 tcgatcagcc tggtcgagat gcccaactac agcctgacgc cggatcgcgc gcggccgagc
     3181 tacgcgctcg gctttgcttc cgagaccatg cgcaactgga tgaacgacct cggcatggac
     3241 gccggcgcct gcaccgcgcc gatcatccgg ttgcgtacgc ggatcgacgc cggcgaattc
     3301 cgcggcacgc tttgggtgca cgcgcgctgc tcgctctggt aaccagggtg ttcgcgatcg
     3361 gggcatagaa tcctcgctgg accaaccagc gaggatcgaa tggacatccg caacaacgtc
     3421 ttcatcgtca ccggcggcgc gtcgggactg ggcggcgcga cgtcgcgcat gattgcggcc
     3481 aacggcggca aggtcgtgat tgccgacgtg caggccgaca aaggcgagcc gctggcacgc
     3541 gaactcggcg gccgcttcgt caagtgcgac gtggcaagcg aggccgacgg caaggcggct
     3601 gtcggcgcgg cgctcgcgct cggcaaactg cgcggactgg tcaactgcgc cggcatcgcg
     3661 atcggcgaaa agacgatcgg caaggacggg ccgcaccggc tcgatgcatt cgtgcgcgtg
     3721 gtcggcgtga acctgatcgg cagcttcaac atgatccggc ttgcggccga cgcaatggcg
     3781 aagcaggaca ccactgccga caacgagcgc ggggtcatcg tcaacacggc gtcggtggcc
     3841 gcgttcgacg gacagatcgg ccaggccgcg tattcggcgt cgaagggcgg catcgtcggc
     3901 atgacgctgc cgatcgcgcg cgatctggcg cgcggcggca tccgctgcgt gacgatcgcg
     3961 cccggcattt tcgccacgcc gatgctgctc ggcatgccgc aggaagtgca ggattcgctc
     4021 ggcaagcagg tgccgttccc atcgcgcctc ggcaagcccg aggagtacgc gcagatggtg
     4081 aaggcgatct tcgaaaacgt aatgcttaac ggcgagacga tccgcctcga cggtgcgatt
     4141 cggctgcagc cgaagtagcg cggccggtcg cggccgagga cgaggcgacc tcatgatgac
     4201 gcgtctcagg gcgacgctgg tcgcgctcgc ggcggcattg cttgccgctg cttgcagcac
     4261 gccgcgggcc gaccgctccg gggcgcccga ggccgcttcc ggcttccgcc tcgggctgac
     4321 cacgcagacg gccacgcgcc atattgccgc gtcggccaac ccactggcga ccgaagccgg
     4381 gcgcgagatc ctgcgcgcag gcggcagcgc gatcgatgca gcgattgcaa tgcagatggt
     4441 gctcaacctg gtcgaaccgc agagctcggg catcggcggc ggcgcgttcc tgctgtactg
     4501 ggatgggagc cgcgtacagg cgtacgacgg ccgcgagacg gcgccgatgg ccgccgacga
     4561 gaacctgttc atggcgggcg gcaagccgat ggcgttctat gacgcagtgg tcggcggtcg
     4621 cagcgtcggc acacccggcg tgctgcgcct gctcgaactc gcgcacaagg agcacggcaa
     4681 gctgccgtgg gcgcggctcc tcgagcccgc gatccgcctt gctgacaacg gcttcacgat
     4741 gtcgccacgg ctcaacacgc tgctggcggg cgagaagtac ctcgcgcgtg acgctgcagc
     4801 gcacgcgtac ttctacagcg ccgacggcac gccgaaggcg gtgggcgcga tgttgcgcaa
     4861 ccccgagttt gcgcagacgc tgcgctcgat cgctgccggc ggcgcggatg cgttctacaa
     4921 gggcagtctt gccgccgaca tcgtgcgcgc cgtacgcgcc catccgaacg caggccagtt
     4981 ggcggagaac gacttcgccg cgtaccaggc caaggagcgc atgccgcttt gcactgacta
     5041 caagcgctgg cgcatctgcg gcatgccgcc accgtcgtcg ggcggcatcg ccgtcgcgca
     5101 gatgctgggc atcttcaccc atcgcaacat cagcgtggtg ccaccgcacg cggtcgacgg
     5161 caggctcgaa ccgcaggtcg acgcggtgca tctgttcagc gaggccggcc ggctcgcctt
     5221 cgcagaccgc aacctgtatg tggccgatcc agactatgtg ccggtcgaca tgctggcgct
     5281 gaccaatccc gcctatctcg ccgaccgcgc gaagttgatc tcggaccgga gcatgggccg
     5341 cgcgcagccc ggcgtgccgg ccggctaccg gatcgcgtgg gcgtcggatc cctcgccgcc
     5401 gcgcatcgcg acctcgcata tctcggtggt cgatcagtac ggcagcgcgc tggcgatgac
     5461 gaccacgatc gaggaccagt tcggtgcgcg catgatggtg catggcttcc ttttgaacaa
     5521 ccagctcacc gacttctcgt tcgtaccgag cgaggatggc aagcccgtcg ccaaccgcgt
     5581 gcagccgggc aagcggccgc gctcgtcgat ggcgccgacc ctggtgttcg atcgcacgac
     5641 gaaccagctc gtcgcgaccg tgggctcgcc gggcggatcg cagatcatcg cctatgtcgc
     5701 caagacactg gtcggcgtcc tcgactggaa cctcgacgtg cagcaggcga tctcgttgcc
     5761 gaacttcggc agtcgcaatg ggcccaccga actcgagcgc gggcgcttcg gcaacccggt
     5821 gctcgaaggg ctgaaggcgc gcgggcacga ggtacgtgag atcgagatga cgagcgggct
     5881 gcaaggcatc gtgcgcgtgc aattgcccga cgggcgtccc ggctgggcgg gaggcgctga
     5941 tccgcgccgc gagggcgtcg cgctcggcga ttaactaaac tcggcgtccc ggacacactg
     6001 gagaaggaca tgacgatcaa ggtcggcgat cggctgcccg agggcacgct gcaggaattc
     6061 atcgaggtcg agacggcagg ctgctcgctc ggtcccaaca cgttcaaggt gcaggacctg
     6121 gtcaagggca agaagatcgc ggtgttcggc ctgcccggcg cctatacgcc gacttgctcc
     6181 gctcaacacg tgccgagcta cgtcaagcac tacgacgccc tgaaagcgaa gggtgtggac
     6241 gagatctggt gcctgtcggt caacgacgcg ttcgtgatgg gcgcctgggg ccgcgaccag
     6301 aaggccaccg gcaaggtgcg catgatggcg gacggttcgg ccgactacac gaagaagctc
     6361 gggctcgaac ttgacctcac cgcgcgcggc atgggcgtgc gttgccagcg cttttcgatg
     6421 ctggtggtcg acggcgtggt caaggcgctg aacatcgaag cacccggcaa gtacgaggtc
     6481 agcggcgcgg agcggatgtt gacgcaggcg tgagatgcgt cacgcccgca tgcgccgcgc
     6541 aatgtcagtt gagccggcgc gcggcgcgct ggaaatgggc gaggcggctg cgcatggtgc
     6601 cggcgtctgg cgcatcgggc gccagcgata gataagcggc aagatcctcg gcggcgccgc
     6661 tcgggcactc caggcgttcg tacaccgcgg cgcggtcacg ccgctggacc gcctcgcggg
     6721 gcagcagcag gatcagccgg ttgagcactt cgagcagcgc cggccagtct tcgttacgcg
     6781 cgtgcgccgc cttcaggttg cgcagccagc gtgccaggat ctcacggtcg gacgccggct
     6841 gcaggtacgc gtcgagcgga agctcggcgc gtccgcgtgt gaacgcatcg acccgctggt
     6901 gcaacacggc ggtcgacagc gttgttccgc attcgaagac gtcgatgaag acgactctgt
     6961 caccggcgcg cagccggacg atgaaatggc ccgggaacgc gacgccgtcg agtccgaggc
     7021 cgatggcgcg gccaagctcc acgtaaagaa ccgacagcga gatcgggatg ccgcggcggc
     7081 gctcgatcac gcggtgcagg tagctgttgt ccgccgagtc atacgtgtcg tggtcgccac
     7141 gaaagccgag ttcgtcgaag aagaagtggt tcagcagccg cagccggttc agcggcgacg
     7201 tgtccgccgc gacgcgcgcg cgcagctgcg cacaccagcg ccgcacgcag ccggcaaccg
     7261 cgtccggatc gaacccggaa tccgcatact gcgcgagatg cgcgacgcag tcgagcagcg
     7321 gcagtggccg gccccgctcg cgctcgatca gccgcgcaaa tcgctcgatc gcgttcaccg
     7381 cgcccgcggc gaaaagtcgc gcaatcgaaa tccgagcagc cacagcaggc cgaagtaaag
     7441 accgagcgcc gctgcgacca cgccgccgag cacgagcgcg cggacgagcc agtccgactg
     7501 catgccgatc cagtcgatcc gacgcgccgc gagccacagc agcaccgcca gcgcagcgag
     7561 cgcgatcgcc atccggatcg agaaccgaag ccatcctggc tgtggcgtat aggtcccgcg
     7621 gcgacgcagg cccgtgaaca gcagcgccgc gttgaccagc gcgccgatgc caaccgacag
     7681 cgcaagccct gcgtgcgcca gcagcggaac caacaccagg ttcgaggcct gcgtggcaag
     7741 caatgaaagc accgcgatct tcaccggtgt cttcacattc tgttgcgcgt aatagcccgg
     7801 tgccaggatc ttgaccgcga tcagaccgag cagtccgacc gcataacccg taagggccag
     7861 cgccgtctgc gcgacgtcgt gggcactgaa cttgccgcct tggaacagca ccgcgatcag
     7921 cccctccgcc agcaggccga gccccagcgc cgccggcagc gcgatcaggc aggtcagtcg
     7981 caacccccaa tcgagcagtc tgctgtagtc gtcggcgcga ccctcggcgc cggccttcga
     8041 cagcgtcggc aacagcacgg tgccgagcgc cacgcccagc agcgcggtcg gaaactccat
     8101 cagccggtcc gcgaacgcga gccaggtcac gctgccgctg gcaaggtgcg aggcgatgtt
     8161 cgtgttgatg atgatcgaca gctgcgcgac cgacactgcg aacagcgccg gcagcatcaa
     8221 ctgcaggatg cgccgcacgc cgggatccgc aaacgccggg cgcaggctgc caatcttcgg
     8281 cagcacgccg agccggcgca gcgccgccgc ctgcaggccg agctgcagcg cgccgccgac
     8341 gaccaccgcg aatgcgagcg ccatgatcgg ccgcgtcagg tacggcgtca gcaccagggt
     8401 gcaggcgatg aacgaaagat tcagcagcac cggcgtgaag gccgggacgg caaagcggtt
     8461 catcgtgttc agcacgcccg ccgcgagcgc gacgagcgac atgaacagga tgtaggggaa
     8521 catccagcgc gtcatcacgg tcgcaagatc gaatgccgcc tgattggccg ccagtcccga
     8581 cgcgatcatc cacaccagcg cgggcgccag cgcgacaccg agcgccgaca ccgcgagcag
     8641 cagccagaac agcgccgagg cgacatggtc gaccagggtg cgcatgccgt cgtcgccgcg
     8701 gctgcggcgt gcgtcggcca gcatcggcac gaaggcctgc tggaacgccc cctcggcaaa
     8761 caatcgccgc agcagattgg gcaggcggaa cgcgacattg aatgcgtcgg tctcggccga
     8821 ggcgccgaag atccgcgaga tcagcaggtc gcgcgcgagg cccgtgacgc gcgacagcat
     8881 cgtcaggctc gagatcgtgg cggcggcgcg gaggagattc aaggatgtgg gggatttgcg
     8941 gcaaaccgag gctgaagctt tgttcgtttg gagccgctct gatatagtct agggcttttc
     9001 cggcgcccct gtttcctctg ggccttcagg cgaccaggcg ccgtctcgaa ctcgggaccc
     9061 gacatggcca ataccaagca ggcgcgcaag cgcgcccatc agaacaccgt tcgcaacgcg
     9121 cacaacacgg cgctgcgttc gcgcgtacgc acggccgtga agtcggtgcg caaggcgatc
     9181 gcggccggcg acaagaaggc ggcgctggaa gtcttccagc aggccaccgg cgtgatcgat
     9241 tcggtcgccg acaagaggat cgttcacaag aacacggccg cgcgcaacaa gagccgcctg
     9301 gcggcggcgc tgaaggcgat gcgctgagct cgaccgccga tccggacggg ggcgcgttcc
     9361 cgcgatgcgg gccgcgcccc ttgcctttgt agcgactact tcgacttcgg cggcggatcc
     9421 ggcagcgaac agccctcgaa cacctgcaac tcgttgtcgc gcgcgaagtt catcacgaag
     9481 tcgaacgcca tccgttcaat gtcgcgcagc cgctcgtcca cgatcacgca cttgacgccg
     9541 ccgaccatcg tcggccggca gtacggcgaa aagccgatca gcgcatcgcg accggcaccg
     9601 accccgccgg ggcggaagcc cgacatcacc ccgcacagtc gctcggccca gtcgctcggt
     9661 cgaaacgtcc tgccgtggat ggtcacgccg tgaatgaaga actcgcgcgg cgcgttcgcc
     9721 tgctgctttg gctcggcggt caagtcggcg agttccggac acggaaagta gcgcgcggat
     9781 tgtatcgtcc gtgaaacgcc acccgcggtg cgcgggtcaa agcaacggca ggtggtcatg
     9841 cgtgtcaacg cggtacgact ccgcgcgttc gaagttccgg aattggcttg cgttcgcggt
     9901 gcatttcgcg cataaagcgt gccgtcccaa ggatccgtcc ctcctccgac ccaggcatgt
     9961 cccccggccc gatatccaag gttctcgtca tcaatgacga gcccctgatt ctcagggaac
    10021 tgctgaaggg cctgaactcc gcggcgcgca cgctcgacaa cccgttcggc atcggcttta
    10081 tcggcgcgct caccgccaag gaggcgctgc accagatcga ggaagatggc gacatccagg
    10141 tggtgatcgt cgacgacaag ctgtacgcgg tgcaggaagg cgcgcggcgc gcggcgaccc
    10201 gctccggcgt gcgcaacggc aacggcaagg aaccatcgcg cagcctgcag gtcagcgcgc
    10261 tgaagctggt gcagaagatc accgcgctgc gcccggagct ggacctgtac atcctgatcg
    10321 agaaggaaaa ggaggaccag atcgtcgatg ctctctttgc cgaggcggtc gatggctact
    10381 tctatcgcga agagcgcgat caccgcggca tgtaccggat cctgaacgcg cagatcgcag
    10441 aacgtgcgcg cacgccgttc tacgacgcgc tgaaagcgta cgtgctcgcc gccaaggacg
    10501 cgtggcacac gccggggcac tcgagcggcg actcgctgcg cggcagtccg tgggtcaacg
    10561 agttctacga gttcatgggt gagcacgtgt tcgatgccga tttgtcggtg tcggtcaagg
    10621 cgctcgactc gctgatggag ccgaccggcg tgatcgcgga ggcacagcag atggcggcca
    10681 aggcgttcgg cgcgcgcaag acgttctttg ccaccaatgg aacgtcgact gccaacaagg
    10741 tgatcctgca gacgctgatc gcgccgggcg agaagatgct gctggaccgc aactgccaca
    10801 agagcgtgca ccacggcgtg gtgctgtcgg gcgcgcggcc gatctatctg gactccagcg
    10861 tcaaccgcaa gtacggcgtg ttcggcccgg tgccgaagaa gacgatcctg aaggcgatcg
    10921 acgaacaccc cgacgcgcaa ctgctggtgc tgacctcatg cacctacgat ggcctcagat
    10981 acgacctgaa gccggtcatc gacgccgcgc acgcgaagaa catcaaggtg ctgatcgatg
    11041 aggcgtggtt ctcgcatgcg cgcttccacc ccgatctgcg gccgacggcg ctcgagtccg
    11101 gtgccgacta cgcgacgcaa tccgcgcaca agacgctgac cgccttctcg caggcggcgt
    11161 tcattcacgt caacgacccg acattcaagg agcatctgtt ccgcgagaac ttcaatatgc
    11221 atacgtcgac cagcccgatg tacagcatga tcgcgagcct cgacgtgggg cggaagcagg
    11281 cggtgctgga gggctacaag ctgctggagc gcacgctgat gctggcggcc gaactgcggc
    11341 agcagatcaa ctcgaccggc gtgtttcgcg tgctcgacct ggacgacctg ctgcccgacg
    11401 aggtcaaggg cgacggcatc cggctggacc cgaccaagat cacggtcgac atctcctcct
    11461 gtggcatgac ggtcgacgaa ctgcagcagg aattgttcac gcgcttcaac atcacgatcg
    11521 agaagtcgac cttcaatacg atcacgctgc tgctcacgat cggcacaacg cgctcgaagt
    11581 gctcgcggct gtacgacgcc ctgatgcgaa tcggccgcga gaagcgcgcg ccgcgccgcc
    11641 tgtaccggac gcccgacctc ccgcccttca ccgaactcgt ctacctgccg cgcgacgcgt
    11701 actactgcgg cggcgaactg atcccgctgc tcgacgacaa cgaccagccc aacgcggcgc
    11761 tgaaggggcg catctgcgcc gaccagatcg tcccctaccc gccgggcatc ccggtgctgg
    11821 tgccggggca gcgcatcacc gagaacatcg tcgagtacct gatccggtat ctgcgcgtgc
    11881 agaacaaggt cgagttgcac ggcgtcgtct accagggcta ctttccgagc gtgcgcgtgc
    11941 tgatggcttc cgaagaacag aagatggcgc cgctcgtgac gaggcgtccg gtgcgcaagg
    12001 cggcctgacc gcgagccgcg cgccggcgcg gcgactcccc accgcgtcgc gcagcggcgc
    12061 ggcaactcca tataattcgc caaacgcggc cgcgccgcgt tttttctttt gttgacgggc
    12121 ccgccatgtc gcacctgatg aacacctatg cccgcctgcc ggtggcgttc acccacggca
    12181 agggcgcgtg gctgtacgac gccgacggca ccgcgttcct ggatgcgctg gccggcatcg
    12241 cggtcaacgg actcggccac ggccacccgc ggctggtcaa ggcgatcgcc gatcaagccg
    12301 ggaagctcat ccacacttcg aacgtctatc gcatccgcga gcaggaggat ctggccgacc
    12361 gcatctgcgc gatcacgggt atggacgaag tgttcttcgc caattccggc tcggaggcca
    12421 acgaaggcgc gctcaagctg gtgcgcttct acggtcacaa gcacggcaac ccggcggcgc
    12481 agacgatcgt gatggaacac gcgtggcacg gccgcaccct cgccacgctc gccgccaccg
    12541 gcagcgagaa ggcacgcaag ggcttcgacc cgttgccgtc cggattcatt cgcgtgccgt
    12601 acaacgatct ggccgcgatc gaaaacatcg cggcgcagga taaacgcgtg caggcgatct
    12661 ggctcgaggt gctgcagggc gagggcggca ttcgggtcgc cgaccgcgac tacgtgcgct
    12721 ccctgcgccg actgtgcgac gagcgcggct ggctgctgat gatcgacgag gtgcaatcgg
    12781 gcatcggccg cacggggaag tggttcgggt accagtgggc cggcatcgct cccgacgtga
    12841 tcacgatcgc caagggtctg ggctcgggcg tgccgatcgg cgcggtggtc gcacgtgggc
    12901 ccgcggcggg cgtcctcggg ccaggaaacc acggttcgac cttcggcggc gggcccctcg
    12961 cgtgccgcgc cgggttggag acgctggcca tcatcgagga cgagaagctg atggccaacg
    13021 ccgacgcaca aggcgagcgg atccgcgccg gattcaagcg cgcactcggc gatcttccag
    13081 gcgtcgccgc catccgcggc caggggttga tgatcggcat cgaactcgac cggccgtgcg
    13141 gcgacctcgc ccggcgcgcg ctcgatgcgc ggctg
//
LOCUS       DBNHBY010000328         2205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1575186, whole genome shotgun sequence.
ACCESSION   DBNHBY010000328 DBNHBY010000000
VERSION     DBNHBY010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1575186"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC16HB_08060"
     CDS             complement(<1..1102)
                     /locus_tag="AC16HB_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase beta subunit"
                     /protein_id="HIR5606785.1"
                     /translation="MTTICVPRDSTALAVGADEVAVAIAAEARRRGIEATIVRNGSRG
                     LFWLEPLVEVATEEGRIGYGPVTLGAVAALFDAGFPQGSAHPLALGRVEALPWLASQQ
                     RLSFARIGVTDPLSLEEYESHGGLAGLRRALSLSREEIVQEVIDSGLRGRGGAAFPTG
                     IKWRTALGAAGDPKYVVCNADEGDSGSFADRLLMESDPFALIEGMAICALAVGATLGY
                     IYVRSEYPHAIAALTKAIAIARAAGLLGTKFDLEVRKGAGSYVCGEETAMLESLEGRR
                     GVVRAKPPLPAIAGLFGRPTVINNVVTLATVPIILARGAAIYRDYGVGRSRGTLTVQL
                     AGNIRRGGLIEAAFGITLRELLMTWGGGTASGR"
     gene            complement(1099..1563)
                     /locus_tag="AC16HB_08065"
     CDS             complement(1099..1563)
                     /locus_tag="AC16HB_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR5606786.1"
                     /translation="MFEPASPTEIAAATSAAIERHAHRAGALLPLLHDVHDRLGYLPA
                     ECEGQIARALNLSRAEVHGVIGFYHHFRRAPPRKPVLHLCQAEACRSMGCQALLAHAQ
                     RTLGHAFEVEPVYCLGQCANAPSALFGERVFARLTPARLDELAAALHATERS"
     gene            1649..>2205
                     /locus_tag="AC16HB_08070"
     CDS             1649..>2205
                     /locus_tag="AC16HB_08070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5606787.1"
                     /translation="MANEARETRVAVMGAGAVGCYYGGMLARAGHAVTLIGRATHVDA
                     VRRDGLLLETQSFRELVRVQAEIDAAAVRGAQLVLFCVKSTDTDAAAAAIAPYLAPDA
                     CVLSLQNGVDNAERLQARLSLEVVPAVVYVATEMAGPGHVKHHGRGELVIARSRSSEG
                     IVRLFGTAGVPVQVSDNVIGALWGKL"
BASE COUNT          340 a          775 c          784 g          306 t
ORIGIN      
        1 gccgacccga cgcggtgccg ccgccccagg tcatcagcag ttcgcgcaat gtgatgccga
       61 atgcggcttc gatcagtccg ccgcgccgaa tgttcccggc gagttgcacg gtcagcgtgc
      121 cgcgcgagcg tccgactccg tagtcgcggt agatcgcggc gccgcgcgca aggatgatcg
      181 gcacggtcgc gagcgtgacc acgttgttga tcacggtcgg ccggccgaac aggcccgcga
      241 tcgccggcag cggcggcttg gcgcgcacca cgccgcggcg gccctcgagg ctttcgagca
      301 tcgcggtttc ttcgccgcac acgtacgagc cggcaccctt gcgcacttcg aggtcgaatt
      361 tggtgccgag aaggccggcc gcgcgcgcga tcgcgatcgc cttggtgagc gcagcgattg
      421 cgtgcggata ctcgctgcgc acgtagatgt agcccagcgt cgcgcccacc gcgagcgcgc
      481 agatcgccat gccctcgatc agcgcgaacg gatcgctctc catcagcagc cggtcggcga
      541 agctgcccga atcgccttcg tcggcattgc acaccacgta tttcgggtcg cccgcggcgc
      601 caagcgcagt gcgccacttg atgccggtcg gaaaggccgc gccgccgcgg ccgcgcaggc
      661 cggagtcgat gacctcctgc acgatctcct cgcgcgacag cgacagcgcg cgcctcagcc
      721 ccgcgaggcc gccgtgcgac tcgtactcct cgagcgacag cgggtcggtg acgccgatgc
      781 gcgcgaagga gagccgctgc tgcgacgcaa gccacggcag cgcctcgacg cggccgagcg
      841 cgagtggatg cgcgctgccc tgcgggaagc ccgcatcgaa gagcgccgcg actgcgccca
      901 gtgtcaccgg gccatagccg atgcgtccct cttccgtcgc gacttcgacc agcggttcga
      961 gccagaacag gccgcgcgaa ccgttacgca cgatcgtcgc ttcgatgccg cgccgacgcg
     1021 cttcggcggc aatcgcgacc gcgacctcgt cggcgccgac cgcgagtgcg gtcgaatcgc
     1081 gcggaacgca gatcgtcgtc atgaccgttc cgtggcgtgc agcgcagcgg caagctcgtc
     1141 cagccgcgcc ggcgtcagcc gcgcgaacac gcgctcgccg aacagcgccg acggcgcgtt
     1201 ggcgcactgg ccgagacagt agaccggctc gacttcgaat gcatggccga gcgtgcgctg
     1261 cgcatgcgcc agcagcgcct ggcagcccat gctgcggcat gcctcggcct ggcacaggtg
     1321 cagtacgggc ttgcgcggcg gcgcgcggcg gaagtggtga tagaaaccga tcacgccgtg
     1381 cacttcggca cgcgacaggt tgagcgcgcg cgcgatctgc ccctcgcact cggccggcag
     1441 ataaccgagc cggtcgtgca cgtcgtgcag cagcggcaga agtgcgcccg cgcggtgcgc
     1501 gtgccgctcg atcgcggccg aggtggcggc cgcgatctcg gtcggcgagg cgggttcgaa
     1561 catggccgaa accatacacc cggaccgtcg ccgcggcgcg agaatgccgc agcagcggcc
     1621 aggatgccgc cggggcggag gtaatcgaat ggcgaacgaa gcacgagaga cgcgggtcgc
     1681 ggtgatgggt gccggcgccg tcggctgcta ctacggcggc atgctggcgc gcgcaggcca
     1741 cgcggtaacg ctgatcggac gagcgacgca cgtcgacgcc gtccgccgcg acggcctgct
     1801 gctcgaaacg cagtcgttcc gcgaacttgt gcgcgtgcag gcggagatcg atgccgccgc
     1861 ggtgcgcggt gcgcagctcg tgctcttctg cgtgaaatcg accgacaccg acgcggcggc
     1921 cgccgcgatt gcgccgtatc tcgcgccgga cgcgtgcgta ctcagtctgc aaaatggcgt
     1981 ggacaacgcc gaacgcctgc aggcacggct gtcgctggag gtcgtgccgg cggtcgtgta
     2041 cgtcgccacc gagatggccg gccccggtca cgtcaagcac cacggccgcg gcgaactcgt
     2101 catcgcccgc tcgcgatcga gcgaaggcat cgtgcggctc ttcggcacgg ccggcgtgcc
     2161 cgtgcaggtg tcggacaacg tgatcggtgc gctgtggggc aagct
//
LOCUS       DBNHBY010000329         2448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1508099, whole genome shotgun sequence.
ACCESSION   DBNHBY010000329 DBNHBY010000000
VERSION     DBNHBY010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1508099"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="AC16HB_08075"
     CDS             complement(<1..128)
                     /locus_tag="AC16HB_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HIR5606788.1"
                     /translation="MDRDQATRFVHDLLKLLLSKKGSDLFLTADFPPAFKIDGRVMP"
     gene            complement(140..1183)
                     /locus_tag="AC16HB_08080"
     CDS             complement(140..1183)
                     /locus_tag="AC16HB_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5606789.1"
                     /translation="MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEH
                     KDVHAMVYDIMNDSQRKVFDENLECDFSFDLPGLARFRVNAFMQDRGAAAVMRTIPSR
                     VLSLEELNCPRVFAEFSLKPRGLVLCTGPTGSGKSTTLAAMCDHVNNHLYGHILTIED
                     PIEFVHESRKCVINQREVGPHTLSFANALRSALREDPDVILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRMVDVFPAAEKEMVRAMLSESLVAVISQSLLKLKDGTG
                     RVAAHEIMVGTPAIRNLIRENKVAQMYSMIQTGSQFGMLTLDQCLMDLVRRNIVAIGE
                     ARTYAKQPETFVG"
     gene            1284..1991
                     /locus_tag="AC16HB_08085"
     CDS             1284..1991
                     /locus_tag="AC16HB_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR5606790.1"
                     /translation="MSTLDETLQANLRRVHERINSAARAVQRTADSVVLVAVSKTVGA
                     ERVVAAARCGQLDFGESYVQEALDKAAQARALAPGLGVRWHFIGPIQSNKTRALAEHF
                     DCVQSVDRLRIAQRLSEQRSVDRSPLDVLLQVNISGESSKSGTVPQEAPALAAAIATL
                     PRLRLRGLMAIPAPTTDARLQREQFARLRMLFDDLRARGLPLDTLSMGMSDDLEAAIA
                     EGSTMVRVGSAIFGARR"
     gene            2004..>2448
                     /locus_tag="AC16HB_08090"
     CDS             2004..>2448
                     /locus_tag="AC16HB_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606791.1"
                     /translation="MTSAAAELDEINRIHDDAPDQAAARLRAFDHAQVPAEKLPLLGF
                     LLNHVLGEKFGRWQESSALLEQLAARADAPIAIARHWAAAADLSSDADATARARARLV
                     AHSAAPEHVCAALARLSTLNWIADAAIHAVEFGQLAQQALGFDRTA"
BASE COUNT          413 a          806 c          817 g          412 t
ORIGIN      
        1 ggcatcacgc gcccgtcgat cttgaacgcc ggcgggaaat ccgccgtcag aaagaggtcc
       61 gaacccttct tgctgagcag cagtttcagc aggtcgtgta cgaatcgggt tgcctggtcg
      121 cgatccatga tctgtcctcc tacccgacga acgtctcggg ctgtttcgcg tacgtacgcg
      181 cctcgccgat ggccacgatg ttgcggcgga ccaggtccat caggcactgg tcgagcgtca
      241 gcatgccaaa ctggctgccg gtctggatca tcgagtacat ctgcgccacc ttgttctcac
      301 gaatgagatt ccggatcgcg ggcgtgccga ccatgatctc gtgcgcggcg acccggccgg
      361 tgccgtcctt cagcttcagc agagactgcg agatcaccgc aacgagggac tccgacagca
      421 tcgcccgcac catctccttc tccgctgcgg ggaacacgtc gaccatgcgg tcgatcgtct
      481 tggcggcgct cgacgtgtgc agcgtgccga acaccaggtg cccggtctcg gcggcagtca
      541 gcgcgagccg gatcgtctcc aggtcgcgca actcgccgac caggatcacg tccgggtcct
      601 cgcgcagcgc cgagcgcagc gcattggcga aactgagcgt gtgcggcccg acctcgcgct
      661 ggttgatcac gcacttgcgg ctttcgtgca cgaactcgat cggatcctcg atcgtcagga
      721 tgtggccgta caggtggttg ttcacgtggt cgcacatcgc cgccagcgtg gtcgacttgc
      781 ccgagccggt cggcccggtg cacagcacca gtccgcgcgg cttgagcgag aactccgcga
      841 acacgcgcgg gcaattgagt tcctccagcg acagcacgcg cgatggaatc gtccgcatca
      901 ccgcggcggc gccgcgatcc tgcatgaacg cgttgacgcg aaagcgcgcc aggcccggaa
      961 ggtcgaacga gaagtcgcac tcgaggttct cgtcgaagac cttgcgctgc gagtcgttca
     1021 tgatgtcgta gaccatcgcg tgcacatcct tgtgctccag cggcgggagg ttgatgcgcc
     1081 tcacgtcgcc atgcacgcga atcatcggcg gcagaccggc cgacaggtgc aggtcggacg
     1141 ccttgttttt gacggagaat gcgagcagtt cggagatgtc catgtgcgct gccggttggg
     1201 ggttggcgcc ggcgctgccg tccggtcccg ctcgccgaaa aattcaaacg agacaagacc
     1261 ctagcaggcg tttcgaaacc attatgtcga ctttggacga aaccttgcaa gcgaatctgc
     1321 gccgagtgca cgaacggatt aactcggccg cccgcgccgt acaacgaacg gccgattccg
     1381 tcgtactggt ggcggtcagc aagaccgtcg gcgccgagag ggtggtcgcg gccgcgcgct
     1441 gcgggcaact cgacttcggc gaaagctacg tgcaggaagc gcttgacaag gccgcgcaag
     1501 cgcgtgcgct tgcgccgggg ctcggcgtgc ggtggcattt catcggaccg atccagagca
     1561 acaagacgcg cgcgcttgcg gaacatttcg actgcgtgca gtcggtcgat cggctcagga
     1621 tcgcacagcg cctgtcggag cagcgcagcg tcgatcgttc cccgctcgac gtgctgctgc
     1681 aggtcaacat cagcggcgag tcctccaaga gcggcaccgt accgcaagag gctcccgcgc
     1741 tcgccgccgc gatcgcgacg ctgccgcggc tgcgactgcg cggtctgatg gcgatcccgg
     1801 caccgacgac cgatgccagg ctgcagcgcg aacagtttgc gcgcctgcgc atgctcttcg
     1861 acgatctgcg cgcgcggggc cttccgctgg acacgctgtc gatgggcatg tccgacgatc
     1921 tggaagccgc gatcgcggag ggttcgacga tggtgcgcgt cggcagcgcg atcttcggtg
     1981 cgcggcgata gactggctgc gcgatgacat ccgccgcagc cgaactcgac gagataaacc
     2041 gcatccacga cgacgcgccg gatcaggccg cggcgcggtt gcgcgcgttc gatcacgcgc
     2101 aggtgccggc cgagaagctg ccgctgctgg ggttcctgct caaccacgtt ctcggcgaga
     2161 aattcggccg ttggcaggaa tcgtcggcgc tgctggaaca gcttgcggca cgcgccgatg
     2221 cgcccatcgc gatcgcgcgc cactgggccg ccgccgcgga cctgagcagc gatgccgatg
     2281 cgacggcgcg cgcacgcgcg cggctcgttg cgcacagcgc ggcaccggag cacgtgtgcg
     2341 ccgcactggc gcgtctgtcg acgctgaact ggatcgccga tgccgcgatt cacgcggtcg
     2401 agttcggaca gctggcgcag caggcgctcg gcttcgatcg gaccgccg
//
LOCUS       DBNHBY010000330         2926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1372850, whole genome shotgun sequence.
ACCESSION   DBNHBY010000330 DBNHBY010000000
VERSION     DBNHBY010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1372850"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="AC16HB_08095"
     CDS             complement(<1..427)
                     /locus_tag="AC16HB_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5606792.1"
                     /translation="MPQTRDLVIAAIALALLPFALTSIGLGVTSATEVVIFALACMAL
                     NILVGYTGLTSFGHGAWFGLAAYAAAMAQRGWFPDGFLLPLATGLAIVFALSWVFGFL
                     ILRRRGVYFSLLTLALAAVLYSVAFRWTEVTGGENGLGGI"
     gene            complement(427..1296)
                     /locus_tag="AC16HB_08100"
     CDS             complement(427..1296)
                     /locus_tag="AC16HB_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606793.1"
                     /translation="MLFLQHLLNGLLAGAYYLLIALGLSLIFALGGIVNLAHGAFYAI
                     GAYLAVTIGERLGFTGAALISPVAVGLLGVVIERLLFRRFYKTDPTLSLLLTFGLAMI
                     AEQALRMIYGAPPLSWSIPPELRGQVIVGDFIYPRFRLLLLAVAVACVAGLWFLLQRT
                     PFGRVVRAGVQNADMVGALGISLEPYMAAVAFLGIGLAALAGVMLAPIFTIHPAMGAE
                     IITSAFVVVVIGGLGSFWGVVLAAVIVGLTKGAVTAVGYPQYSEAAIYLLMLLVLLAR
                     PRGLFGERIQRFE"
     gene            complement(1347..2591)
                     /locus_tag="AC16HB_08105"
     CDS             complement(1347..2591)
                     /locus_tag="AC16HB_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606794.1"
                     /translation="MSQRRSFSRRTVIKGAGAAAVGVAFPYISYGQSDVIRIGHLTPR
                     TGFLGPLGEFAVQAADMAVAEINAAGGVNGRKIDLLKEDSVNPQTASTNVERMIERDK
                     VACIIGEISSASGLTISQVAERTKTLFINSGCNSDELRGKGCKKYMFHIESQNSMYVK
                     TCGRSLMAQGLVKGRKWFSLTADYAFGHDLLRVAKKFMAANGGDFAADKLVPTDVTDF
                     SPLMLEIRNAKPDLVISNLAGNQITNFLKQYSEFGLPFPVAGFGFDTAVAWAAGKGNF
                     IGTWPLVWHHLIDTPASKQFVADFTKRYNRPPENQAWGDYNAIKLTAQAMAEIKSTDS
                     TRLVEHFEKGAKFGLMKTREGYFRKSDHQMMHEMYTVQGLPAAQIKNQWDIFTSSPPV
                     PGPNESLEVIATTAEENECKMA"
     gene            2720..>2926
                     /locus_tag="AC16HB_08110"
     CDS             2720..>2926
                     /locus_tag="AC16HB_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5606795.1"
                     /translation="MSSAIAKRRGTAKRAHTRSVRPTMGGQVHGRLCKLLVSGVLSPG
                     EKLSLRTVAERLGVSITPVRAAVAR"
BASE COUNT          542 a          964 c          965 g          455 t
ORIGIN      
        1 cgatgccgcc gaggccgttc tcgccgccgg tgacctcggt ccagcggaac gcgaccgagt
       61 acagcaccgc ggcgagcgcg agcgtcagca gcgagaagta cacgccgcgc cggcgcagga
      121 tcaggaagcc gaagacccac gacagcgcga agacgattgc caaccccgtc gcgagcggca
      181 ggaggaagcc atccggaaac cagccgcgct gcgccatggc ggccgcgtac gcggcgaggc
      241 cgaaccacgc gccgtggccg aacgaggtca gccccgtgta gccgaccagg atgttcagcg
      301 ccatgcaggc gagtgcgaag atgacgacct cggtcgccga tgtgacgccg aggccgatcg
      361 aggtcagcgc gaagggcagc aatgccagcg cgatggccgc tatgacgagg tcgcgggtct
      421 gcggcatcac tcgaaccgct ggatccgctc gccgaacagc ccgcgcgggc gggcgagcag
      481 caccagcagc atcagcaggt agatcgcagc ttccgagtac tgcggatagc cgaccgcagt
      541 cactgcgccc ttggtgaggc cgacgatcac cgcggccagc acgacgcccc agaacgatcc
      601 gagcccgccg atcactacga cgacgaaggc ggacgtgatg atctccgcgc ccatggccgg
      661 atggatcgtg aagatcggcg ccagcatcac gcccgccagc gctgccagcc cgatgccgag
      721 aaacgcgacc gcggccatgt atggctcgag cgagatgccg agcgcgccga ccatgtcggc
      781 gttctgcacg ccggcacgca ccacgcgacc gaacggcgtg cgctgcagca ggaaccacaa
      841 acccgcgacg cacgcgaccg cgaccgcgag cagcagcagg cggaagcgcg gatagatgaa
      901 gtcgccgacg atcacctgac cgcgcaactc gggcgggatc gaccacgaca agggcggcgc
      961 gccgtagatc atccgcagcg cctgctcggc gatcatcgcg agtccaaagg tcagcagcag
     1021 cgacagcgtc gggtcggtct tgtagaagcg gcggaacagc agccgctcga tcacgacgcc
     1081 gagcagtccg accgccaccg gcgaaatcag cgccgcgccg gtgaagccga gccgctcgcc
     1141 gatcgtgacc gccagatacg cgccgatggc atagaacgcg ccgtgcgcca gattgacgat
     1201 cccgccgagc gcgaagatca gcgacagccc aagcgcgatc agcaggtaat acgcacccgc
     1261 gaggagtccg ttgaggagat gctgaagaaa gagcaaggag agattcgagg cgtcgccccg
     1321 gttcaggccg gggcgacgtt ctcaggtcac gccatcttgc actcgttctc ttccgccgtg
     1381 gtcgcgatca cctccagcga ctcattcggc cccggcaccg gcggcgacga agtgaagatg
     1441 tcccactggt tcttgatctg cgcggccggc agaccctgca cggtgtacat ctcgtgcatc
     1501 atctggtggt cgctcttgcg gaagtagcct tcgcgggtct tcatcaggcc gaatttcgcg
     1561 cccttctcga agtgctcgac cagcctggtc gaatcggtcg acttaatttc cgccatcgcc
     1621 tgcgcggtca gcttgatcgc gttgtagtcg ccccacgcct ggttttccgg cgggcggttg
     1681 tagcgcttgg tgaagtcggc cacgaattgc ttcgatgccg gcgtgtcgat caggtgatgc
     1741 cacacgagcg gccaggtgcc gatgaaattg cccttgccgg cggcccaggc gaccgcggtg
     1801 tcgaagccga agcccgcgac cgggaagggc aggccgaact cggagtactg cttcaggaag
     1861 ttggtgatct ggttgccggc gagattcgag atcacgaggt ccggcttggc gttgcggatc
     1921 tccagcatca gcggcgagaa gtcggtgaca tcggtcggta ccagcttgtc ggccgcgaaa
     1981 tcgccgccgt tggcggccat gaacttcttc gccacgcgca gcaggtcatg accgaatgca
     2041 tagtcggccg tcagcgagaa ccacttcctg cccttgacca gtccctgcgc catcagcgaa
     2101 cggccgcagg tcttcacgta catcgagttc tggctctcga tgtggaacat gtacttcttg
     2161 cagcccttgc cgcgcagttc gtcggagttg cagccgctgt tgatgaagag cgtcttcgtg
     2221 cgctcggcga cctggctgat cgtcaatccc gacgccgacg agatctcgcc gatgatgcat
     2281 gcgaccttgt cgcgttcgat catccgctcg acattggtcg acgcggtctg cgggttgacc
     2341 gagtcctcct tcagcagatc gatcttgcgc ccgttcaccc cgccggcggc gttgatctcc
     2401 gcgaccgcca tgtcggcggc ctgcaccgcg aactcgccga gcgggccgag gaagcccgtg
     2461 cgcggcgtca aatgaccgat gcggatgacg tcggactggc cgtagctgat gtacggaaag
     2521 gcgacgccaa cggcggccgc gccggcgccc ttgatgacag tgcggcgcga aaacgaacgt
     2581 ctctgactca cgatctcctc cttgctgtgc cccttgggat tgccggaccg aggcgcgccg
     2641 gccgcgcttg ccgaaacggt gattaccgct tgcttgcggc aaactgtgat cacaattatc
     2701 gtggatcaag gaggcggatt tgtccagcgc gatcgcgaaa cgccggggca cagcgaaacg
     2761 ggcgcacacg cgttcggtgc gcccgaccat gggtggacag gtgcacggcc gcctgtgtaa
     2821 gttgctggtc tccggcgtac tctcgccggg cgagaagctg tcgctgcgca cggtggccga
     2881 acgcctcggc gtatccatca cgccggtgcg cgccgcggtc gcgcgg
//
LOCUS       DBNHBY010000331        20368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1439706, whole genome shotgun sequence.
ACCESSION   DBNHBY010000331 DBNHBY010000000
VERSION     DBNHBY010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1439706"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC16HB_08115"
     CDS             complement(<1..682)
                     /locus_tag="AC16HB_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5606796.1"
                     /translation="MARLARLSIAGHPHHVVLRGGHGMAIFRSADEYGFFLDSLRVAA
                     REKHVAVHAYVLMPDHVHLLVTPATAAALGGVMQSLGRRYVRWFNRRHHREGALWDGR
                     YRSSVIEASQYLLDCCCYIETNPVRAGLIADAANYPWSSLAHHLGLVADPLVSDHPVV
                     WSLGNTPFERQSAYRELLARPLAVERIERLRWGAKGGWGVGAPDFIAELERASKRPLS
                     PRTRGRKRK"
     gene            complement(695..1831)
                     /locus_tag="AC16HB_08120"
     CDS             complement(695..1831)
                     /locus_tag="AC16HB_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR5606797.1"
                     /translation="MSATVELGLAPFAAHADHSRGRRHAEPLAQARTDFQRDRDRIVH
                     STAFRRLEYKTQVFVNHEGDLFRTRLTHSLEVAQIARTIARHLRVNEDLVEAISLAHD
                     LGHTPFGHAGQDALNESMQPYGGFEHNLQSLRVVDELEERYGAFNGLNLTFETREGIL
                     KHCSLEKARALGELGLRFIERRQPSLEAQIANLADEIAYNNHDVDDGLRSGLITLKQI
                     EEIPLFARHAGDVRREFPDIAERRMINETTRRMINALIVDLIGETRGRTTAVAPQSID
                     EVRAGPTLVAFSDAMRAEADQLKRFLTEHLYRHYQVMRMTEKARRIVRDLFAAFRDEP
                     RLLPTDHRQRVESEGPRAIADYVAGMTDRYAIREHRRLFTVGDG"
     gene            complement(1828..2907)
                     /gene="aroB"
                     /locus_tag="AC16HB_08125"
     CDS             complement(1828..2907)
                     /gene="aroB"
                     /locus_tag="AC16HB_08125"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR5606798.1"
                     /translation="MHRITVQLAGRPYDILVGSGLLTRLARWVAPLQPSRLFVVTNPV
                     IDGFHGRTVRDSLAALAPLHSVLLPDGETFKTMDSVQHVLDELVSAGADRKSLIVALG
                     GGVIGDIAGFAAAIYMRGIRFVQVPTTLLAQVDSSVGGKTGVNHPRGKNLIGAFHQPA
                     VVVSDTDTLKTLPTRELSAGLAEVIKHGLLADERYLDVVERDLPLLLECDPGALTEAI
                     AGSCRIKAAVVGRDERESGERALLNLGHTFAHAIESLTGYGTWLHGEAVGCGTCLAAD
                     LSARCGLIDKGAVARIEKAVRAARLPTRITGLPKREAVASMRGDKKSESGRIRFVLLE
                     RIGRAIQREVPEDLLDATLTAGGFT"
     gene            complement(2946..3524)
                     /locus_tag="AC16HB_08130"
     CDS             complement(2946..3524)
                     /locus_tag="AC16HB_08130"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR5606799.1"
                     /translation="MCGLILFPASDSPVIQPGQRIFLIGMMGVGKSTIGRLLARETGL
                     EFIDSDRELEARSGVTIATMFEVEGEESFRRREAMLLDELTQRRGVVLATGGGAVLQP
                     ENRERLHSRGLVIYLESTVDEILRRTQADKARPLLQAADRRARIEELLQYRRPLYEAT
                     AHLTFHSGMFNPKKLAARVLENELVRNTVGMA"
     gene            complement(3561..5282)
                     /locus_tag="AC16HB_08135"
     CDS             complement(3561..5282)
                     /locus_tag="AC16HB_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5606800.1"
                     /translation="MAEATRIREFAGGALTTVRRGLALVAAIGMLASCGGNAPGGDTT
                     GGGTTGGAAAVDTVEVSTSKSAMAARADESVTITVTTLDGSRRGIPGANVQLSADSGV
                     ISSASTVTDSGGKLTATFTLGQDRSNRTVTVTASSGGSSGSATIQVTGTTVTLSSNTS
                     VVSDATASAQLTARLLDAASQPISGAVVQLSTTQGTLSATSATTDAGGVATANITGIS
                     SSATITARAGNTASSVSILASGSSNVSPEPAGIQIQDFIIQANPAVTGPNQAGNTGNF
                     SVLEVRVFGSLAGTPNLPVKNAPVRFRIASTPALGVLEVDTSSNPALTNEVGLAANRF
                     IPGAATSGTDFIVVCASVSGLAVPATPGVDFTGNPCSASEKAVRLTIAQQALFVRISV
                     DNKLSATDGGLTRTNQFIVSVTDAAGRGVPGVLITPRLLPLHYYKGFFVFDATEGVAQ
                     WVPQLHLQCANEDTNFNGTLDATDNNLNGDAFVWPGQIAAVSMDNGGITDSTGFVVGR
                     AKFGARYGTWADYQLEVRASVGGTEGLATEFFSLGVLVDEVKNKDIAPTWVVSPFGTA
                     GSCSSPN"
     gene            complement(5323..7491)
                     /locus_tag="AC16HB_08140"
     CDS             complement(5323..7491)
                     /locus_tag="AC16HB_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR5606801.1"
                     /translation="MNRLWSSMWNAVVVAAAMGMTLLPSTVAAQAAANSIERVDATQT
                     GAGVVLTIQLKSDVAGIPPSFSIANPARIAIDLPQTTNNLGRNALELNQGDLRSVNVV
                     QAQDRTRVVLNLKRSLAHSISVSGNTVQVALGAVADTTTFRAPAAPAATTAAAAAPAA
                     AAGPRTLKAIDFRRGADGEGRVVVDLSDPTTSVDIRQQGQQIIVDFLGTSVPETLRRR
                     LDVTDFGTPVAMVNTTQQGSNVRMVIEPRGLWEQNAYQSDTRFVVEVKPVKEDPTRLF
                     QGTRPGYQGERLSLNFQNVDIRSLLQVIADFTDLNIITSDSVQGSITLRLKDVPWDQA
                     LDIILQSKGLDMRKNGNVLVVAPREELATKEKLELEARSQIADLEPLRTENFVINYQK
                     ADDVRRLLVEGQQRMLSKRGSAVVDTRTNQLFVQDTTTRLEEIRRLIQRVDIAVPQVL
                     IEARIVEASDTFSRNLGVRLGGSTGYPREIGGTNAFGTIGGVGGAISTSGPGGPVTIG
                     NGGTSVITTPNVASGANFVNLPAAGISGFGPAGASLTLFNSDLTRFINLEITALEAEG
                     QGKIVSSPRVITADKVKATIEQGTEIPYQQATSSGATSVSFRKATLKLEVTPQITPEG
                     AIFLDVKVNKDSRGQDTTSGPAIDTKNVQTQVLVENGGTVVLGGIYEQNERTTTTKVP
                     LLGDIPGLGWLFKNRVRINDRTELLIFITPRVVSSQLAGR"
     gene            complement(7488..8021)
                     /locus_tag="AC16HB_08145"
     CDS             complement(7488..8021)
                     /locus_tag="AC16HB_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR5606802.1"
                     /translation="MLVPVVVAACSAAGEAEIRQWMADVRQGMKPTTEPVPPPKEFTP
                     FAYDSRGLVDPFDSQKVVMAVARQQQARSTGSSIRPDLDRRRETLEGFSLDQIRMVGM
                     MKQGGTNVALLETGGATHLVRVGNYVGQNFGLVTRISETELQLKEIVQDAAGEWVERP
                     AKLELQESAGRQQGGKR"
     gene            complement(8054..8698)
                     /locus_tag="AC16HB_08150"
     CDS             complement(8054..8698)
                     /locus_tag="AC16HB_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR5606803.1"
                     /translation="MASINFRELGEQFKGLTLDNVGTWPVLPRITVWLFVIAMCAVLG
                     WLLLWSKQSEELDRLRTEEDALKGQYRQKLQQAINLEELRKQKEQVSQYVLALEKQLP
                     SKAEMDALLSDINQAGIGRGLQFELFRPGGVNIRDYYAELPISVRLSGRYHDLGAFAS
                     DIANLPRIVTLNNITLQSLRDGALAMDATAKTYRYLDEEELFKQRQARQAQAKK"
     gene            complement(8716..9342)
                     /locus_tag="AC16HB_08155"
     CDS             complement(8716..9342)
                     /locus_tag="AC16HB_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR5606804.1"
                     /translation="MRIRINLLPHREARRERQKNAFYFLTLLSLIAGAALVFVVWSVF
                     QGLISGQQDRNNFIAAENRKLDLQIREIANLRQEIDALRARQRAVEDLQADRNQPVYL
                     LDELTKQVPEGIYLRQIKQEDKKVNVTGQAASNERVSELLRNLQSNSRFLERPELIEI
                     RIAGNQPANVSRRLFDFSLNLYLKPTPERAQLTQAQQAALPPSTAPKK"
     gene            complement(9339..10418)
                     /locus_tag="AC16HB_08160"
     CDS             complement(9339..10418)
                     /locus_tag="AC16HB_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR5606805.1"
                     /translation="MAFELPFLSSKTPPLIGLDIGSSSVKIVELSEPSVGAVRLERYA
                     IEPLPRGAVVDGNIDKIDIVAEAVKRAWRRSGTRFKNVAMALPASAVITKRIALPANL
                     REQELEMQVESEANQYIPFALDEVSLDFQVLGPIPNAPDDVEVLIAASRKEKVEDRMA
                     VAQAAGLKPVIVDVESYAMRSALQRLINQLPNSGEGLVIAVFYMGANSTSVTVMHDGE
                     AIYEREQPFGGQQLTGDIARTYGIPADEAEQKKRSGDLPANFETDVLKPFVDSAVTEI
                     TRALQFFFTSTPYTRIDQIMLAGGSAVLAGLADALMERAQVPTAVISPFKGMEISPNI
                     REKQLRLDAPALLTPCGLAMRRFDE"
     gene            10906..13203
                     /locus_tag="AC16HB_08165"
     CDS             10906..13203
                     /locus_tag="AC16HB_08165"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR5606806.1"
                     /translation="MTAGGLLVAGGIALFLLLSFIFALAYSSLPNIEALTDYRPKIPL
                     RVWTSDGVLIGEFGEERRDFVAIKDVPQRIREAVLAAEDDAFYRHSGVDYLGLVRAAL
                     SNFASGRRGQGGSTITMQVARNFYLSSERSYLRKVYEIALAYKIESQLTKDKILEVYI
                     NQIFLGQRAYGFAAAAQVYFGKKLPEISVGEAALLAGLPAAPSAYNPWANPRRAKARQ
                     QYVLSRMLALGFIDKPAYETALAEPFRQRIAAAVFSPQPAKSRLPAEYAAEMARQIMF
                     DVFQDETYTRGLNVYTTLVSTEQQTAYNALRAQVLEYDRRYGYRGPEAFIELEDDAHA
                     REQQFEDALLEAIDSPDLFPAVVIEANPKLVKVVVQGGEQMEIKGDGLRFAARALDPK
                     AQPGRRIVPGAVVRVSRSAKGGWEISQLPQVEAAFVSASSDDGAVRALVGGFDFNLNK
                     FNHVTQAWRQPGSSFKPFIYSAALEKGFMPSTLVNDAPIVIDPKLTGGQLWEPKNYDG
                     KYEGPMRLRQGLAKSKNLVSIRVLQAIGPRYAQEYITRFGFEADRHPAFLTMGLGAGS
                     VTPWQMLDGYAVFANGGYLVKPYLISKVTDANGAVLMQAQPVRAGETAPRVVDERNVY
                     VMDSLMRDVARYGTAARAASLKRADTAGKTGTTNDSHDAWFAGYSGGIVAVSWMGFDQ
                     PRPLGDRETGGGLALPIWINYMSTALKGRAEIQRPMPEGVVSVGGEIYFSEFQPGQGV
                     ASVGLEDTLAPEEKRKADAVREQIF"
     gene            complement(13200..13523)
                     /gene="cyaY"
                     /locus_tag="AC16HB_08170"
     CDS             complement(13200..13523)
                     /gene="cyaY"
                     /locus_tag="AC16HB_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIR5606807.1"
                     /translation="MTESEFLALADSTLDAIERAIEMSGADIEVARSGNVLTLELADG
                     SRIVVNSQTPMRQIWIAAKSGGFHFERTGERWCDTRDGVELFASLSRIVSAQGGQPVV
                     LGAAH"
     gene            13538..13729
                     /gene="lptM"
                     /locus_tag="AC16HB_08175"
                     /gene_synonym="yifL"
     CDS             13538..13729
                     /gene="lptM"
                     /locus_tag="AC16HB_08175"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR5606808.1"
                     /translation="MSRVIGILALALGTLVSCGQKGPLYLPGQSPKNAPLPPPAQTQP
                     APESQTRAPDVPASSDSKK"
     gene            13726..14994
                     /gene="lysA"
                     /locus_tag="AC16HB_08180"
     CDS             13726..14994
                     /gene="lysA"
                     /locus_tag="AC16HB_08180"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5606809.1"
                     /translation="MSSDAPAAFARRDGTLWCEEVSLAAVAQRFGTPTYVYSRRAIER
                     AYGEYAAAVAGRPALICYAMKANSNLAILNLLARRGSGFDIVSGGELARVLAAGGDPR
                     KAVFSGVGKSEAEIEQALRAGILCFNLESAPELQMIERVAARLGTHARISVRVNPDVD
                     PSTHPYIATGLKGNKFGVSYAETLPLYRRAAEMQYIEVVGIDCHIGSQITALAPYVDA
                     AERVLDLVDVLEREGIRLHHIDFGGGLGIRYRDETPPDPASLIGAVLARVDARGHGAK
                     TVMFEPGRSIVGNAGALLTRVNIIKRGEERNFAIVDAAMNDLLRPALYGAWMDVQPVQ
                     PPDAAPLTVDIVGPVCESGDWLARERELAVEPGDLLAVLGAGAYGMTMASNYNSRPRA
                     AEVVVDGSAVHCVRQRETIEELFAREAMLP"
     gene            complement(14943..15653)
                     /locus_tag="AC16HB_08185"
     CDS             complement(14943..15653)
                     /locus_tag="AC16HB_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR5606810.1"
                     /translation="MGARSVATATASSQLLARVPTVLAPTTFSRLRSLLFAVALLPLA
                     RLIWLGLIGALGANPVEFVIRSLGTWALVMLCVTLAVSPLRWLTGWAWLLRLRRMLGL
                     FCFFYASLHVFAYFGLDQWFALGAILKDIAKRPYITIGFASYLLLIPLAVTSTNAMAR
                     RLGARNWQRLHRTVYVIAPLAVLHFWWQKAAKNNVGEPALYALLVGLLLGARVLHRWR
                     TRQGSMASRANSSSIVSR"
     gene            complement(15655..16602)
                     /gene="msrP"
                     /locus_tag="AC16HB_08190"
     CDS             complement(15655..16602)
                     /gene="msrP"
                     /locus_tag="AC16HB_08190"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR5606811.1"
                     /translation="MLIRHPADIAPSEITPPAVWQKRRAFARAAAALGLIGLAMSLRA
                     QPARKLDAKPGAFSTMERPTPFDFATSYCNFYEFGAEKEDPPRNAPSRLRTRPWTIRV
                     DGEVGKPRTFDIDDLMKLAPLEERIYRLRCVEAWSAVIPWMGFSLADLVRRVEPTGNA
                     KYVEFTTLADAKQMPGLSAHVLDWPYVEGLRMEEAMHPLTLLTFGMYGVTLPNQNGAP
                     VRIVVPWKYGFKSAKSLVRIRFVEKQPLNSWQKSAPNEYGFYSNVNPTVDHPRWSQAT
                     ERRLGEDGLFAKRRPTQMFNGYADQVASLYGGMDLKKYF"
     gene            16725..17774
                     /locus_tag="AC16HB_08195"
     CDS             16725..17774
                     /locus_tag="AC16HB_08195"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIR5606812.1"
                     /translation="MHIEHEVKLDYGDVLIRPKRSTLTSRSDVDIAREFDFRHAKVRY
                     HGIPIIAANMDSTGTFEMARALDPFGLSTALHKHYDDAQYIEFFRMLERKSASFYSIG
                     ITRADEEKFRRVMQASPSDAPAVQYVCIDVANGYHENFVTFVSRIRAAWPQLVIMAGN
                     VVTGEMTEELILSGADIVKVGIGPGSVCTTRKMTGVGYPQLSAVIECADAAHGLGGLI
                     CADGGCTTPGDVAKAFGGGADFVMLGGMFAGHDEAGGETVERDGRALRRFYGMSSRAA
                     MEKYAGGVAEYRAAEGKEVLVPYRGPVANTVQEILGGVRSACTYVGARRLKELSKRTT
                     FVRVTQQVNEVFGNI"
     gene            complement(17815..18465)
                     /locus_tag="AC16HB_08200"
     CDS             complement(17815..18465)
                     /locus_tag="AC16HB_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5606813.1"
                     /translation="MISRTGRGLTSGFALIWVTVAMTASAQDKAVAKVDPGKGQQIAA
                     AVCTACHASDGNSIISANPKLAGQNSVYLYRQLTDFTKPADDKSARVSSVMAGFAGQL
                     SDADKRNVAAYYASQTPKPGTARNRETLQFGQRIYRAGIPEKSVPACAGCHSPNGAGI
                     PIQYPRLGGQHAEYTETQLKAFRDGTRRNSVPMMQIAARLSDAEIKAVADYVNGLR"
     gene            18485..19288
                     /gene="yihA"
                     /locus_tag="AC16HB_08205"
     CDS             18485..19288
                     /gene="yihA"
                     /locus_tag="AC16HB_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR5606814.1"
                     /translation="MRSCRRPAAAVLPSGSRRRLPGAATAYSTQVPSSSRSARAPRPE
                     GRHTDHGPLFESATFALSVAELADINAARLALLPEIAFVGRSNAGKSSVINVLTRQRR
                     LAFASKTPGRTQLLNFFVLSGRGPSDALEPCAYLVDLPGYGFARVDDATRKRWDRFIG
                     GYLQTRRMLAGVVLVIDARRGLTVADEDLLAWIAGRARPEVLHLHLLLTKCDQLTTRA
                     RRDAHAAAEQRAAALPMPTSVQLFSALKREGVDELRTVLTHVITEGARV"
     gene            19441..>20368
                     /gene="hemB"
                     /locus_tag="AC16HB_08210"
     CDS             19441..>20368
                     /gene="hemB"
                     /locus_tag="AC16HB_08210"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR5606815.1"
                     /translation="MHILGQYPAARMRRMRRDDFSRRLMREHQVTVDDLIYPVFILDG
                     QKRREAVDSMPGVERLSIDNLLPVAEEALQLGVPVLALFPVIEGGFKTADGREATNPE
                     GLVPRAVRALKQRFPELGLLTDVALDPYTTHGQDGLIDADGYVLNDETVTVLRHQALI
                     QAQAGVDIVAPSDMMDGRIGAIRAALEEAGYIHTRIMAYAAKYASAFYGPFRDAVGSK
                     GNLGKSNKAVYQMDPANSDEALLEVGLDLAEGADMVMVKPGMPYLDIVRRVKDEFRAP
                     TFVYQVSGEYAMLMAAARNGWLDEKKVILEALL"
BASE COUNT         3616 a         6604 c         6435 g         3713 t
ORIGIN      
        1 gtttcctctt gcggcctctt gttcttggag atagcggacg cttacttgca cgttcgagtt
       61 ctgcgatgaa gtccggtgca ccaactcccc acccgccctt tgcgccccat cgcaatctct
      121 caatacgctc caccgccagc ggacgggcga gcagttcgcg gtaggcggac tgtcgctcaa
      181 acggcgtgtt gccgagcgac cagacgaccg ggtgatcgga cacaagcggg tcggcgacca
      241 agccaaggtg atgggctagg ctcgaccagg gatagttcgc cgcgtcggcg atgagtccgg
      301 cgcggacagg attcgtttcg atgtagcagc agcagtcgag caggtactgg gacgcttcga
      361 tcacgctgga tcgatagcgg ccatcccaca gtgcaccctc gcgatggtga cgccggttga
      421 accagcgcac gtagcgtcgc ccgagcgact gcatgacgcc gccgagcgcg gccgcggtcg
      481 caggggtaac cagcagatgc acgtgatccg gcatcagcac gtaggcgtga accgccacgt
      541 gcttttcccg cgcagcgacg cgcagggaat cgaggaagaa accgtattcg tcggcgctgc
      601 gaaagatcgc catgccatgg ccgccccgca ggaccacgtg gtgcggatgc ccggcaatgc
      661 tcagacgcgc gagccgtgcc atggtggagc cggatcagcc gtcgccgacg gtgaacagcc
      721 ggcgatgctc gcggatcgca tagcgatcgg tcatgccggc aacgtaatcg gcaatcgcgc
      781 gcggaccctc gctttcgacg cgctggcggt ggtcggtcgg cagcaggcgc ggctcgtcgc
      841 ggaacgctgc gaacaggtcg cgcacgatcc gccgcgcttt ctcggtcatc cgcatcacct
      901 gatagtggcg atacaggtgt tcggtgagaa atcgcttcag ctgatcggct tccgcgcgca
      961 tcgcatcgga gaacgcaacc aatgtcggtc cggcccgcac ttcgtcgatc gactgcggtg
     1021 ccacagcggt tgtccgaccc cgcgtttcgc cgatcaggtc gacgatcagc gcgttgatca
     1081 tgcgccgggt cgtctcgttg atcatgcgac gttcggcgat atccgggaac tcacgccgga
     1141 catcgccggc gtgccgagca aagagcggaa tctcctcgat ctgcttcagc gtgatcaggc
     1201 ccgagcgcag tccgtcgtcg acgtcgtggt tgttgtaggc gatctcgtcg gcgagattgg
     1261 cgatctgcgc ctcgagcgat ggctggcggc gctcgatgaa gcgcaaacca agttcgccca
     1321 gcgcgcgcgc cttttcgagc gagcagtgct tgaggatccc ctcacgcgtc tcgaaggtca
     1381 gattcaggcc gttgaaggcg ccataacgtt cctcgagctc atccacaacg cgcaacgact
     1441 gcagattgtg ttcaaagccg ccgtacggct gcatcgattc gttcagcgcg tcctgccctg
     1501 cgtggccgaa cggcgtgtgt cccaggtcgt gcgcgagcga gattgcctcg accaggtcct
     1561 cgttgacgcg cagatgacgt gcgatagtgc gcgcgatctg cgccacctcg agcgagtgcg
     1621 tgagccgcgt acggaacagg tcgccttcgt ggttgacgaa cacctgcgtc ttgtattcga
     1681 gccgacgaaa cgcggtggaa tggacgatgc ggtcgcggtc gcgctggaaa tcagtgcggg
     1741 cctgcgcgag cggctcggcg tggcgccggc cccgcgagtg gtcggcatgg gccgcaaagg
     1801 gcgccagacc gagttccacc gtggcgctca tgtgaagccg cccgccgtca gcgtggcgtc
     1861 cagcagatcc tccggcactt cgcgctggat ggcgcgcccg attcgctcca gcagtacgaa
     1921 acgaatccgc cccgactcgg acttcttgtc gccgcgcatc gatgccacgg cttcccgctt
     1981 cggcaggccc gtgatgcgcg tcggcagcct tgctgcccgc acggctttct cgatgcgcgc
     2041 gacggcgccc ttgtcgatca ggccgcagcg cgcggacagg tcggccgcaa ggcacgttcc
     2101 gcagccaact gcttcgccgt gcagccaggt tccatagccg gtcaacgact cgatcgcatg
     2161 ggcgaacgtg tgaccgaggt tcagcagtgc gcgctcgccg ctttcccttt cgtcgcgccc
     2221 gacgaccgcg gccttgatcc ggcaggagcc cgcgatcgcc tccgtcagcg cgcccgggtc
     2281 gcactcgagc aggagcggca gatcacgttc gacgacatcc aggtagcgct cgtcggccag
     2341 cagcccgtgc ttgatgacct ccgcaaggcc agccgacagt tcccgcgtcg gcagtgtctt
     2401 gagtgtgtcc gtgtcggaaa cgaccacggc cggctggtga aacgcgccga taagattctt
     2461 tccgcgcgga tgattgacgc ccgtcttgcc acccaccgac gaatcgacct gggccagcag
     2521 ggtcgtcggc acctggacga agcggatgcc gcgcatatag atcgcagccg cgaaacccgc
     2581 gatatcgccg atcacgccgc cgccaagcgc gacgatcagg ctcttgcggt cggcaccggc
     2641 gctgacgagt tcatcgagca catgctgaac cgagtccatc gtcttgaacg tctcgccgtc
     2701 ggggagcaaa accgaatgca aaggtgcgag cgctgcgagg gagtcgcgta ccgttctgcc
     2761 gtgaaatccg tcgatgaccg gattcgtgac gacgaacaag cgtgacggct gcagcggcgc
     2821 aacccagcgc gcgagccgcg tcagcaggcc gcttccgacg agaatgtcgt agggccgacc
     2881 cgccagttgg acggtgatcc gatgcacgcg acgattttaa gcggccctgc gtatgaaagc
     2941 cgcattcatg ccatgccgac cgtgtttcgc acgagttcgt tttcaaggac gcgggccgcc
     3001 agcttcttcg gattgaacat gcccgagtga aacgtcaggt gcgcggtggc ttcgtaaagc
     3061 ggacgtcggt actgcagcag ttcctcgatg cgcgcgcgtc ggtcggctgc ttgcagcagc
     3121 ggccgcgcct tgtcggcctg ggtgcggcgc aggatctcgt cgacggtcga ctcgagatag
     3181 atcacgagtc cgcgcgaatg cagccgctcg cgattttccg gctgcagcac cgcgccgccg
     3241 ccggtcgcca gcacgacacc tcgccgctgt gtcagctcgt cgagcagcat cgcttcgcgc
     3301 cgtcgaaacg attcctctcc ctcgacttcg aacatcgtcg cgatcgtcac gccgctacgc
     3361 gcctccagtt cgcggtcgct gtcgatgaac tccagccccg tctcacgcgc cagcaagcgg
     3421 ccgatggtcg atttgccgac gcccatcatg ccgatcagaa agatgcgttg cccgggctgg
     3481 atcaccggcg aatccgatgc agggaaaaga atgaggccgc acatggcggc ctcgagaaca
     3541 atccggcgcg tatcagtcga tcaattcgga ctgctgcacg aaccagccgt tccgaaagga
     3601 ctgaccaccc aggtcggagc gatgtccttg ttcttcacct cgtcgaccaa cacgcccagc
     3661 gagaagaact ccgttgccaa gccttcagtg ccgccaaccg atgcacgcac ttcaagctga
     3721 tagtcggccc aggtgccgta acgagcgccg aatttcgcgc gcccgacaac gaaaccggtc
     3781 gaatccgtga tgccgccatt gtccatcgac accgcggcga tctgcccagg ccagacaaac
     3841 gcgtcgccgt tcaggttgtt gtccgtcgca tcaagcgtgc cattgaagtt ggtgtcctca
     3901 tttgcgcatt gcagatgcaa ctgaggcacc cactgagcaa cgccttccgt cgcatcgaag
     3961 acgaagaaac ccttgtagta atgcagcggc aagagtcgcg gcgtaatcag cacgcccggc
     4021 actccgcggc ctgcagcatc ggtgacgctc acgatgaact ggttggtgcg cgtcagacca
     4081 ccgtcggtgg cactcagctt gttatcgacg gaaatcctga cgaacagcgc ctgctgcgca
     4141 atggtcaggc gcacagcctt ctcgctcgct gaacacggat tgcccgtgaa atccacgcca
     4201 ggcgtcgccg gcacggccag acctgacacc gaggcacaga caacgatgaa gtccgtcccg
     4261 gacgttgcgg ccccggggat aaagcgattg gcagccagtc cgacctcatt ggtcagtgcc
     4321 gggttgctcg acgtatcgac ctcgagcacg ccaagtgccg gcgtcgatgc gatccggaaa
     4381 cgaacggggg cgttcttcac tggcaaattc ggtgtgcccg caagggagcc gaacacgcgc
     4441 acctcgagca cggagaagtt gccggtgttg ccggcctgat tcgggccggt caccgcggga
     4501 ttcgcctgaa taatgaagtc ctggatctgg attccggccg gctccggaga aacgttggat
     4561 gatccggagg caagaatgga cacggagctc gcggtgttcc ccgcgcgcgc cgtgatggtc
     4621 gccgacgagc tgatgccggt gatattggcc gtggcgacgc cgcccgcatc ggtcgttgcg
     4681 ctcgtcgcac tcaacgttcc ctgcgttgtg ctcagttgca ccaccgcacc gctgatcggc
     4741 tggcttgccg catcaagcag ccgcgcggtg agctgtgcgg acgcagttgc atcgcttacc
     4801 acactggtgt tggacgacag cgtcaccgtc gttcccgtta cctggatcgt ggcgctcccg
     4861 ctcgaaccgc cgctcgatgc agtgactgtc acggtccggt tcgagcgatc ctgacccaaa
     4921 gtgaacgtgg cagtcagttt gcctccgcta tcagtcaccg tactagcgga cgaaatcacc
     4981 cccgagtctg cggacaattg aacgttggcg ccagggatcc ctcgccgcga tccatcgagt
     5041 gtcgtgaccg tgatcgtcac cgattcgtcc gcacgcgccg ccatcgcact cttcgacgtc
     5101 gagacctcca cggtgtctac cgccgcggcg cctccggtcg ttccgcctcc cgtcgtatca
     5161 ccgcccggcg cattcccacc acaagacgcg agcatgccga tggcggcaac aagtgccagt
     5221 ccgcggcgta cggtcgtcaa cgccccacca gcgaattccc gaattcgagt cgcctcagcc
     5281 atgtccacac tcttcaaacg attgaacaat gtcccagtca cttcagcgac ccgccagttg
     5341 gctcgatacc acgcgcggag tgatgaagat cagcagttcg gttctgtcgt tgatgcggac
     5401 ccggttcttg aacagccagc cgaggccggg gatgtcgccg agcaacggca ccttcgtcgt
     5461 cgtcgtgcgc tcgttctgct cgtagatgcc gccgagaacc actgttccgc cgttctcgac
     5521 gagtacctgg gtctgaacgt tcttcgtgtc aatggccgga cccgacgtcg tgtcctgccc
     5581 gcggctgtcc ttgttcacct tgacgtccag gaagatcgct ccctccggcg taatttgcgg
     5641 ggtcacttcg agctttagag tggccttgcg gaatgaaacg ctcgtcgcac cgctcgaggt
     5701 cgcttgctgg tacgggatct ccgtgccctg ctcgatcgtc gccttcacct tgtccgccgt
     5761 gatcacgcgc gggctcgaaa cgatcttgcc ctgcccctct gcctcaagcg cggtgatctc
     5821 gaggttgatg aagcgcgtga ggtccgagtt aaacagggtc aacgatgcgc cggccggccc
     5881 aaagccgctg atgccagccg caggcaggtt cacgaagttc gctcccgagg cgacattcgg
     5941 ggtcgtgatg accgacgtgc ctccattgcc gatggttacc ggaccgcctg gaccgctggt
     6001 agatatcgcg ccaccgacgc cgccgatggt gccgaatgcg ttggtaccgc cgatctcacg
     6061 cggatagccg gtactgccgc ccaagcgaac cccaagattg cgactgaaag tgtcggacgc
     6121 ctcgacgatg cgcgcttcga tcaggacctg cggcaccgcg atgtccacgc gctggatcag
     6181 gcgacgtatt tcctccagcc gggtcgtggt gtcctgcacg aacagctggt tggtgcgggt
     6241 atccacgacg gcgctgccgc gcttcgacag catgcgctgc tgaccttcga ccagcaggcg
     6301 gcgtacgtcg tccgccttct ggtagttgat gacgaagttc tcggtgcgca acggttcgag
     6361 atccgcgatc tggctgcgtg cctcgagctc gagtttctcc ttggtggcaa gctcctcgcg
     6421 cggcgcaacg acgagcacgt tgccattctt gcgcatgtcg aggcccttgc tctgcaggat
     6481 gatgtcaagg gcctgatccc acggcacgtc cttcagccgc agcgtgatcg agccttgcac
     6541 cgagtcgctg gtgatgatgt tcagatccgt gaagtccgcg atcacctgca gcagcgagcg
     6601 aatgtcgacg ttctggaagt tcagcgacag ccgctcgccc tggtagcccg gccgcgttcc
     6661 ctggaacagc cgcgtcgggt cttccttgac cggcttgacc tcgacgacga agcgcgtatc
     6721 gctctggtag gcgttttgtt cccatagccc gcgcggctcg atcaccatcc gcacgttgct
     6781 gccttgctgg gtcgtgttga ccatcgccac cggcgtgccg aagtcggtca cgtcgaggcg
     6841 gcggcgcaac gtctccggca cgctggtgcc gaggaagtcc acgatgatct gctgcccctg
     6901 ttggcggatg tcgacgctcg tcgtcggatc gctcagatcg acgaccaccc ggccctcgcc
     6961 gtcggcgcca cgccggaaat cgatcgcctt cagggtgcgc ggtcctgcgg cagcggcggg
     7021 tgccgcggcg gcggccgtcg tcgcagccgg cgcggcgggt gcacggaacg tcgtcgtgtc
     7081 agcgactgca ccgagcgcga cctgcacggt gttgccggac acgctgatcg aatgggcgag
     7141 cgatcgcttc agattcagca cgacccgcgt gcgatcctgc gcctgcacca cattcacact
     7201 gcgcagatcg ccctggttca attccagcgc atttcgtccg aggttgttcg tcgtctgcgg
     7261 caggtcgatt gcgatccgcg ccggattggc aatcgagaaa ctgggtggaa tcccggcgac
     7321 gtcggatttc aactgaatcg tcagcacgac gcctgcgcca gtctgcgtgg catcgacgcg
     7381 ctcgatgctg ttggctgccg cctgcgccgc cacggtcgac ggaagcaacg tcatgcccat
     7441 cgccgccgca accacgaccg cgttccacat cgaactccaa agcctgttca tcgtttaccc
     7501 ccttgctggc gcccggcgct ctcctgcaac tccaacttgg cgggtcgctc gacccactcc
     7561 cccgcggcgt cttgcacgat ttccttcagt tgaagttctg tctccgaaat gcgcgtgacc
     7621 aggccgaagt tctggccgac atagttgccg acccgcacca gatgtgtcgc gccgccagtc
     7681 tcgaggagcg caacgtttgt gccgccctgt ttcatcatgc cgaccatgcg aatctggtcg
     7741 agcgaaaaac cttccagcgt ctcgcgtcgc cggtccagat cgggccgaat cgagctgccg
     7801 gtcgagcgcg cctgctgctg gcgcgccacc gccatcacga ccttttgcga gtcgaatggg
     7861 tccaccaggc cgcggctgtc gtatgcgaac ggcgtgaatt cctttggcgg cggcaccggc
     7921 tcggtcgtcg gcttcatgcc ctgacggacg tcggccatcc actgccggat ctccgcctcg
     7981 cccgcggcgc tgcatgccgc gacgaccacc ggaaccagca tcaatgcaag cagacgcagt
     8041 ctcatcatcc gtcctatttc ttcgcctgcg cctgccgcgc ctgacgctgc ttgaacaact
     8101 cttcctcatc gaggtaccgg tacgtcttgg ccgtcgcgtc catcgccagc gcgccgtcgc
     8161 gcagagattg gagagtgatg ttgttcagcg tgacgatgcg cggcaggttc gcgatgtcac
     8221 tcgcgaacgc gccgaggtcg tgataacggc ccgacaggcg caccgagatc gggagttcgg
     8281 cgtagtagtc gcgaatgttg acgcccccgg gccggaacag ctcgaactgc aggccgcgcc
     8341 caatgcccgc ctgattgatg tcggaaagca gcgcgtccat ctcggctttg ctcggcaact
     8401 gtttctcgag tgccagcacg tactggctga cctgttcttt ctgcttgcgc aactcctcga
     8461 gattgatcgc ctgctgcagc ttctgccggt actgtccctt cagcgcatct tcctcggtcc
     8521 gcaggcggtc cagctcttcg ctctgtttgc tccaaagcag cagccagccg agcacggcgc
     8581 acatggcgat cacgaagagc caaacggtga tgcgcggcag gaccggccac gtaccgacgt
     8641 tatcgagcgt cagccctttg aactgctcgc ccagctcgcg gaaattgatc gatgccatct
     8701 tcgtcccctg cgcccctact tcttcggtgc cgtcgatggc ggcagcgccg cctgctgagc
     8761 ctgcgtcagc tgcgcgcgtt ccggtgtggg cttcagataa aggttcagcg agaaatcaaa
     8821 caggcggcgc gacacgttag ccggctggtt acccgcgatc cggatctcga tcagttcagg
     8881 ccgttccagg aacctggagt tcgattgcag gttgcgaagc agttcggaca cgcgctcatt
     8941 cgaggctgcc tggccggtca cattgacttt cttgtcctcc tgcttgatct gtcggaggta
     9001 gatgccctcc ggcacctgct tggtcagctc atcgagcagg taaacgggtt gattgcggtc
     9061 cgcctgcaga tcctcgaccg cgcgttgacg cgctctcagc gcatcgattt cctgccgcag
     9121 attcgcgatc tcgcgaatct gcaggtcgag cttgcgattc tccgccgcga tgaagttgtt
     9181 gcggtcctgc tgcccgctga tcagcccttg gaacacggac caaacgacga acaccagcgc
     9241 ggcaccggcg atcaggctga gcagcgtgag gaagtagaaa gcgttcttct gccgctcgcg
     9301 cctggcctcg cggtgtggca gaaggttgat gcgaattctc attcgtcgaa tctccgcatc
     9361 gccaggccgc acggcgtcag cagcgccggc gcgtcgagcc gcaactgctt ttcgcgtatg
     9421 ttgggagaga tctccatgcc cttgaacggc gaaatcaccg ccgtcggcac ttgggcgcgt
     9481 tccattagcg cgtcggccag cccggccagc acggcggacc cgcccgcaag catgatttgg
     9541 tcgatgcggg tgtacggcgt ggaggtgaag aagaactgca gtgcccgcgt gatctcggtg
     9601 acagccgaat cgacgaacgg cttcagaacg tccgtctcga aattcgccgg caagtcgccg
     9661 ctgcgcttct tctgctccgc ctcgtcggcg ggaattccgt acgtgcgagc gatatcgccc
     9721 gtcaattgct gtccgccgaa tggctgttcg cgctcataga tggcctcgcc gtcatgcatc
     9781 acagtgacgc tcgtcgagtt cgcacccatg tagaagacgg cgatgacgag tccttcgccg
     9841 ctgtttggca gctggttgat cagtcgctgc agcgcactgc gcattgcata cgactccacg
     9901 tcgacgatga cgggcttcag acctgcggcc tgcgccacgg ccatgcggtc ctcgaccttc
     9961 tccttgcgcg aagccgcaat cagcacctcg acatcatcgg gcgcgttcgg aatcggaccg
    10021 agcacctgga agtcgaggct gacctcatcg agagcgaacg ggatgtactg attcgcttcg
    10081 ctctcgacct gcatctcgag ctcctgctca cgcaggttgg caggtaatgc gatgcgcttg
    10141 gtgatcacgg ccgaggccgg cagcgccatc gcgacgttct tgaagcgcgt gcccgaccga
    10201 cgccatgccc gcttcaccgc ttcggcgacg atatcgatct tgtcgatgtt gccgtcgacg
    10261 acggcgccgc gcggcaacgg ctcgatcgca tagcgctcaa gccgcaccgc accgacgctc
    10321 ggctcggaca gttcgacgat cttcacactc gacgatccga tgtccagacc gatgagaggc
    10381 ggcgtcttgg atgacagaaa cggcaactcg aaggccaagg tggcactccc gataacgcac
    10441 tgcttctcga tcgatgctcc ggatctccgg agagaaaggc ggcggtctag acaagaaacc
    10501 acgacgcgtc atctgctgcg cgtcgtccga cgtttttcct tatctttcga gcgtttacgg
    10561 cttctttagt ggatgctact tcaaatcgta tcaacggaac gaaaagaacg acaagcccta
    10621 cgaatggacg atcggcggcg gtgccgcctt gacgctcgca cgaaccgctg aactttggcc
    10681 gcctcgcaga ttcgaattga cgatccaagg cttcggggct ccatacctat aattcgcacc
    10741 attccctctt gtcgccgatt gtgactgcct ccaggcaacc acgccacgcg ttcgctggcc
    10801 ttaggcggtc cgaagtgcat cgtgtccgct gattctcgtc atcgcgaagt ctcgcgcacg
    10861 caacagttca cggctctctt gcgatcgccg tgggcgcggc tgggaatgac tgcgggcgga
    10921 ctcttggttg ccgggggcat tgcactgttc ctgctcctgt ccttcatttt tgctcttgcg
    10981 tattcgagcc tgcctaatat cgaggcgctc acggactatc ggccgaagat cccgctgcgg
    11041 gtttggacct ccgatggcgt gctgatcggc gaattcggcg aagaacgtcg cgatttcgtt
    11101 gccatcaagg atgtccccca acgcatcagg gaggcggttc tcgccgccga ggacgacgcc
    11161 ttttaccgtc acagcggagt cgattacctg ggcctcgtgc gagcggcgtt gtcgaatttc
    11221 gcttcgggtc ggcgcggcca gggcggcagc accatcacga tgcaggtcgc gcgcaacttc
    11281 taccttagct cggagcgcag ttatctgcgc aaggtttacg agatcgcgct ggcgtacaag
    11341 atcgagtcgc agctgaccaa ggacaagatt cttgaggtgt acatcaatca aatcttcctc
    11401 ggccagcgcg cgtatggctt cgccgcggcg gcgcaggtct acttcggcaa gaagctgccg
    11461 gagatttcgg tcggcgaggc ggccttgctg gccggcctgc ctgcggcacc gagcgcctac
    11521 aacccttggg ccaacccgcg ccgcgccaag gcacgccagc agtacgtgct ctcgcgcatg
    11581 ttggcgctcg gtttcatcga caagcccgcc tacgagacag cgcttgccga gccatttcgt
    11641 cagcggatcg cggccgcggt cttctcgccg cagccggcca aatcgcgttt gcccgcggag
    11701 tatgcggccg agatggcgcg ccagatcatg ttcgacgtat tccaggatga aacgtacacg
    11761 cgcggcctga acgtctacac gacgctggtc agcaccgaac agcagacggc atacaacgcg
    11821 ctgcgggctc aagtgctcga atacgaccgc cgctatggct accggggacc agaggcattc
    11881 atcgaactgg aggacgatgc gcatgcacgc gaacagcagt tcgaggatgc gctgctggag
    11941 gcgatcgaca gtccggatct gttcccggcg gtagtgatcg aagccaatcc caagctggtg
    12001 aaggtagtcg tgcagggcgg cgagcaaatg gagatcaaag gcgatggcct gcgcttcgcg
    12061 gcgcgcgcgc tcgatccgaa agcccagcct ggccggcgca tcgtgcccgg ggccgtcgtg
    12121 cgcgtttcgc gctcggccaa gggggggtgg gaaatctctc aactcccgca ggttgaggcc
    12181 gccttcgtgt cggcctcgtc cgacgacggt gcagtgcgcg cgctggtggg cgggttcgac
    12241 ttcaacctga acaagttcaa ccacgtgacg caggcgtggc gccagcccgg ttcgagtttc
    12301 aagccgttca tctattcggc ggcactcgag aagggcttca tgccgagcac gctggtgaac
    12361 gacgcgccga tcgtgatcga cccgaaactc accggcggtc agctgtggga gccgaagaac
    12421 tacgacggca agtacgaggg cccgatgcgg ctgcgtcaag gtctggccaa atcgaagaac
    12481 ctggtgtcga tccgcgtgct gcaggcgatc ggcccgcgct atgcacagga gtacatcacc
    12541 cgattcgggt tcgaggccga ccgacatccg gcgttcctga cgatggggtt gggggcgggg
    12601 tcggttacac cgtggcagat gctggacggc tatgcggtgt tcgccaacgg cggctacctc
    12661 gtcaagccgt acctgatcag caaggtcacc gacgcgaacg gtgcggtgct gatgcaggcg
    12721 cagcccgtca gggcgggcga aacggcgccg cgtgttgtcg acgagcgcaa cgtgtacgtg
    12781 atggacagtc tgatgcgcga cgtggcgcgc tatggcacgg cggcgcgcgc cgcgtcgttg
    12841 aagcgcgcgg acaccgctgg caagaccggt acgaccaacg attcgcacga tgcgtggttc
    12901 gccggttaca gcggaggcat cgttgccgtg agctggatgg gcttcgatca gccgcgtccg
    12961 ctgggtgatc gcgaaaccgg cggcgggctg gcgctaccga tctggatcaa ctacatgagc
    13021 accgcactga aaggtcgggc cgagatccaa cggccgatgc cggaaggtgt cgtcagcgtg
    13081 ggcggcgaga tctatttctc ggaattccag cccggacaag gcgtcgcgag cgtgggcctc
    13141 gaggatacgt tggcgccgga ggaaaagcgc aaggcagacg ccgtgcgcga gcagatcttc
    13201 tagtgcgcgg cgcccagaac gaccggctgc ccgccttgcg cggacacgat ccgcgaaagc
    13261 gatgcgaaca attccacgcc gtcgcgcgtg tcgcaccagc gttcgcccgt gcgctcgaag
    13321 tgaaatcctc cgctcttggc cgcgatccag atctggcgca tcggcgtctg gctgttgacg
    13381 acgatccgcg aaccgtcggc cagttcaagc gtcaacacat tgccgctgcg cgcaacctcg
    13441 atgtccgctc cggacatttc gatcgcgcgc tcgattgcat cgagcgtgct atcggccagc
    13501 gccaggaatt cgctttcggt catgatagat tgacccgatg agtcgggtaa tcggcatttt
    13561 agcgttggcc ctcggcacac tggtcagttg cggccagaag ggcccgctgt acctgccggg
    13621 ccagtcgccg aagaacgcgc ctttgccgcc gcccgcgcaa actcagccgg cgcccgaatc
    13681 gcagacgcgc gcgccggacg tccctgcttc ctccgacagc aagaaatgag ctccgacgcc
    13741 cccgccgcgt ttgcacggcg cgacggcacg ctgtggtgtg aagaggtctc gctcgccgcg
    13801 gtcgcgcagc gtttcggcac accgacatac gtgtattcgc gacgcgcgat cgagcgcgcc
    13861 tatggcgaat atgcggccgc agtcgcaggc cggccagcgc tcatctgcta cgcgatgaag
    13921 gccaactcga acctcgcaat cttgaacctg ctggcacgca ggggcagcgg gttcgatatc
    13981 gtgtctggcg gcgaactggc gcgcgtgctc gcggcgggtg gcgatccgcg caaagccgtt
    14041 ttctccggcg tcggcaagag cgaggcagag atcgagcaag cgctacgcgc cggcatcctg
    14101 tgcttcaacc tcgaatcggc gcccgaactg cagatgatcg agcgcgtggc cgcgcgcctg
    14161 ggtacgcatg cccgcatatc ggtgcgcgtg aacccggacg tcgatccaag cacgcatccg
    14221 tacatcgcga cgggactgaa gggcaacaag ttcggcgttt cgtatgccga gacgctgccg
    14281 ctttaccggc gtgcggcgga gatgcagtac atcgaggtgg tcggcatcga ctgccacatc
    14341 ggctcgcaga ttaccgcgct cgcaccctac gtcgacgcag cggagcgggt gctcgacctc
    14401 gtcgatgtgc tcgaacggga aggaatccgc ctgcaccaca tcgatttcgg cggcgggctg
    14461 ggcatccgct atcgggacga gacgccgccc gacccggcat cgctgattgg cgccgtgctt
    14521 gcgcgcgtcg acgcgcgcgg gcacggggca aagaccgtga tgttcgagcc gggacggtcg
    14581 atcgtcggca acgcgggtgc gcttctgacg cgggtgaaca tcatcaagcg cggcgaggag
    14641 aggaatttcg cgatcgtcga cgcggcgatg aacgatttac tgcgaccggc gctttacggc
    14701 gcatggatgg acgtgcaacc ggtgcagccg cccgatgcgg cgccgctgac ggtcgatatc
    14761 gtcggaccgg tgtgcgagtc cggcgactgg ctcgcgcgcg agcgcgaact cgcggtcgag
    14821 ccgggcgacc tgctcgcggt gctcggcgcc ggcgcgtacg gaatgacgat ggccagcaat
    14881 tacaactcgc gtccgcgcgc cgccgaggtc gtcgtcgacg gcagcgcggt gcactgcgtg
    14941 cgtcaacgcg agacgatcga ggaactgttc gcgcgcgaag ccatgctgcc ttgacgcgtg
    15001 cgccatcggt gcagcacgcg cgcgccgagc agcaggccaa ccagcagcgc gtacagtgct
    15061 ggctcgccaa cgttgttctt cgccgccttc tgccaccaga agtgcagcac tgccagcggc
    15121 gcgatcacgt agaccgtccg gtgcagccgc tgccagttgc gtgcgccgag ccgtctcgcc
    15181 atcgcgttgg tcgacgtcac cgcgagcgga atgagcagca ggtacgacgc aaacccgatc
    15241 gtgatgtacg ggcgcttggc gatgtccttc aggatcgccc cgagcgcaaa ccactggtcg
    15301 aggccgaagt acgcaaacac gtgcagcgac gcgtagaaga agcagaacag acccaacatg
    15361 cgacgcagcc gcagcagcca cgcccatccc gtcagccaac gaaggggaga cacggccagc
    15421 gtcacgcaca acatcacgag cgcccatgtg cccagcgagc ggatcacgaa ctcgacggga
    15481 ttggctccga gcgcgccgat caacccgagc cagatcaacc gcgccagcgg cagcagcgca
    15541 accgcaaaca gcagcgaacg caaacgactg aaggtcgttg gtgccagcac agtcggcacg
    15601 cgagcaagca actgcgaact cgcagttgcg gtcgccaccg acctcgcgcc catttcagaa
    15661 atacttcttc aggtccatgc caccgtaaag tgacgcgacc tgatccgcat agccgttgaa
    15721 catctgcgtc ggacggcgct tcgcgaacag gccgtcctcg ccgaggcggc gttcggtcgc
    15781 ctggctccag cgtggatggt cgacggtcgg attgacgttc gaatagaagc catattcgtt
    15841 gggcgcagac ttctgccagc tgttcagcgg ctgcttctcg acgaagcgga ttcgcacgag
    15901 cgacttcgca ctcttgaaac cgtacttcca cggcacgacg atccgcaccg gcgcgccatt
    15961 ctggttcggc agcgtgacgc cgtacatgcc aaaggtgagc agtgtcagcg gatgcatcgc
    16021 ctcctccatc cgcaggccct cgacatatgg ccagtcgagc acatgcgcgc tgagacccgg
    16081 catctgcttc gcatcggcca gcgtcgtgaa ttcgacgtac ttggcattgc cggtcggttc
    16141 cacgcgtctc accagatcag caagcgaaaa ccccatccac ggaatcaccg ccgaccatgc
    16201 ctcgacgcag cgcagtcgat agatgcgttc ctcgagcggt gcgagcttca tcaggtcgtc
    16261 gatgtcgaat gtgcgcggct tgccaacctc gccgtcgacc cggatcgtcc acggccgcgt
    16321 acgcaggcgc gatggcgcgt tgcgcggcgg atcttccttc tcggcgccga actcatagaa
    16381 attgcagtaa ctggttgcga aatcgaacgg cgtgggccgc tccatcgtcg agaacgcccc
    16441 gggtttggca tccagcttgc gcgccggctg tgcgcgcagc gacatggcaa ggccgatcag
    16501 cccgagcgct gccgccgcgc gcgcgaaggc gcgtcgcttc tgccacaccg cgggcggtgt
    16561 tatctccgaa ggggcgatgt cggctggatg gcgaatcaac actcgagact ccgctttagg
    16621 ctgtgtggtc gacccggtac gtgcgacatt acacgcgagc ggtttgagcc cgttccaacg
    16681 gtacaggttc cgcggcacta gacttccggt atcggagact tgccatgcac atcgaacacg
    16741 aagtcaagct cgattacggc gacgtgctga tccggcccaa gcgctcgacg ctgacatcgc
    16801 ggtcggacgt ggatatcgcg cgcgagttcg acttccggca cgccaaggtg cgctaccacg
    16861 gcatcccgat catcgcggcg aacatggact caaccggaac gttcgagatg gcgcgcgcgc
    16921 tcgatccatt cggcctgtcc actgcccttc acaaacacta tgacgacgcg cagtacatcg
    16981 aattcttccg catgctggaa cgcaagtcgg cgtcgttcta ttccatcggc atcacccgcg
    17041 cggacgagga gaaattccgg cgcgtgatgc aggcatcgcc cagcgacgcg ccggccgtgc
    17101 agtacgtgtg catcgacgtg gccaacggct atcacgagaa cttcgtcact ttcgtatcgc
    17161 gtatccgcgc cgcttggcca cagcttgtga tcatggccgg caacgtagtc acgggcgaga
    17221 tgaccgagga actgatcctg tcgggcgccg acatcgtcaa ggtcggcatc ggacccggct
    17281 cggtgtgcac gacacgcaag atgaccgggg tcggctatcc gcagctgtcg gccgtgatcg
    17341 agtgtgcgga cgctgcgcac ggactcggcg gactgatctg cgccgacggt ggttgcacga
    17401 cgccgggcga cgtggccaag gcgttcggcg gcggcgcgga cttcgtgatg ctcggcggca
    17461 tgtttgccgg ccacgatgaa gcaggcggag agaccgtcga gcgcgacggg cgcgcgctgc
    17521 ggcgctttta tggcatgagc agccgcgccg cgatggaaaa gtacgcaggc ggcgtggcgg
    17581 agtatcgcgc ggccgagggc aaggaggtgc tggttcccta tcgcgggccg gtcgccaaca
    17641 cggtgcagga gatcctgggc ggcgtgcgct cagcttgcac ctacgtcggc gcgcgccggc
    17701 tcaaggaact gtccaagcgc accaccttcg tgcgtgtgac tcagcaggtc aacgaagtct
    17761 tcggcaatat ctgagacttg cggacgcggg gcgcagcgcc gcgggctctt tcccctatct
    17821 caagccgttg acgtagtcgg ctaccgcctt gatctctgcg tcggacagac gcgcggcgat
    17881 ctgcatcatc ggcacgctgt tgcgccgagt cccgtcgcgg aaggccttca gctgagtttc
    17941 cgtgtattcg gcgtgctggc cgccgaggcg cggatactgg atcggaattc cggcgccgtt
    18001 cgggctatgg cagcccgcgc aggccggtac ggacttctcg gggatgcccg cgcgatagat
    18061 ccgctgaccg aattgcagcg tctcccgatt gcgcgccgtt ccgggcttgg gcgtctgcga
    18121 tgcatagtaa gcagcgacat tgcgcttgtc cgcatcggac agttggccgg caaatccagc
    18181 catcacgctg ctgacgcgcg ccgacttatc gtccgccggc ttggtgaaat ccgtcagctg
    18241 ccggtagaga tacacggaat tctggccggc gagcttggga ttggcactga taatcgagtt
    18301 gccatcggac gcgtggcaag cggtgcagac ggcagcggcg atctgctgtc ctttgccggg
    18361 atcgaccttg gcgaccgcct tgtcctgcgc gcttgccgtc atcgcgaccg tcacccagat
    18421 caacgcgaaa ccagacgtca ggccacggcc agtacgtgaa atcatcgaga cactttccag
    18481 gaagatgcga tcttgtcgtc gtcccgccgc agcggtcttg ccgtcgggca gtcggcgccg
    18541 attgccgggc gcagcgaccg cgtattctac ccaagtgccc tcgtcttccc gttcagcacg
    18601 cgcgccacgc ccggagggcc ggcacaccga tcacggcccg ctcttcgagt cggcgacgtt
    18661 cgcgctttcc gtggccgaat tggctgatat caacgcggcg cgactcgcgc tactgcccga
    18721 gattgcgttc gtcggccgct ccaatgccgg caagtcgagc gtgatcaacg tgctgactcg
    18781 gcagcggcgg ctcgcgttcg cgagcaaaac cccgggccgc acgcagttgc tgaacttctt
    18841 cgtgctgagc ggtcgcggcc cgagcgatgc gctggaaccg tgcgcctacc tggtcgacct
    18901 gcctggctat ggtttcgcgc gcgtggatga tgcgacgcgc aaacgttggg accgctttat
    18961 cggcggctat ctgcagacgc ggcgcatgct cgccggcgtc gtgctggtta tcgatgcccg
    19021 ccgcgggctg acggtagcgg acgaggatct gctggcatgg atagccggcc gcgcgcgacc
    19081 cgaggtgctg cacctgcacc tgctgctgac caaatgcgac caactcacga cgcgcgcgcg
    19141 ccgtgacgcg cacgccgctg ccgaacagcg cgccgctgcg ttgccgatgc ccacgtcggt
    19201 tcagctgttt tccgcgctga agcgcgaagg cgtggacgaa ctgcgcacgg tgctgaccca
    19261 cgtgattacg gagggggcgc gcgtatgatc gatgcggcaa aaaaaacccc gagacgttcg
    19321 ctcggggcgc aagccttctc gtcaaaggcg cccgctcagg gaggagaagc gggggacgac
    19381 gaatcgtccg gatcacactg taccgttcac gacgcaaaag actatttggg attttcccga
    19441 atgcacatcc tcggccagta cccagcagca cgcatgcgcc gcatgcggcg cgacgacttc
    19501 tcgcgccgcc tgatgcgcga acatcaggtc actgtcgacg atctgatcta tccggtcttc
    19561 attctcgacg ggcagaagcg ccgcgaagcg gtcgattcga tgccgggcgt cgaacgattg
    19621 tcgatcgaca acttgctgcc ggtcgcagaa gaagcgctcc aactcggcgt tccagtgctc
    19681 gcgctctttc ccgtaatcga aggagggttc aagaccgccg acggccgcga agcgaccaac
    19741 ccggagggcc tggtgccgcg cgccgtacgc gcgctgaagc agcgctttcc cgaattgggc
    19801 ctactgaccg acgtcgcgct cgatccgtac acgacccacg ggcaggacgg actgatcgat
    19861 gccgacggct acgtgctcaa cgacgaaacc gtcacggtgc tcagacacca ggcgctgatt
    19921 caggcgcagg ctggcgtgga catcgtcgcg ccaagcgaca tgatggatgg ccgcatcggc
    19981 gccatccgcg ccgctcttga ggaggcgggc tacatccaca ctcgcatcat ggcgtatgca
    20041 gccaagtatg ccagcgcgtt ctacggtccg ttccgggacg cggtcggctc gaagggcaat
    20101 ctgggcaagt cgaacaaggc cgtctaccag atggacccgg ccaacagcga cgaagcgctg
    20161 ttggaagtcg gcctcgatct ggccgaagga gccgacatgg tgatggtcaa gcctggcatg
    20221 ccgtaccttg acatcgtgcg gcgcgtgaag gacgagttcc gcgcgccgac cttcgtctac
    20281 caggtcagcg gcgagtacgc gatgctgatg gccgccgcgc gcaacgggtg gctggacgag
    20341 aagaaggtga tccttgaagc gctgctcg
//
LOCUS       DBNHBY010000332         1784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_831011, whole genome shotgun sequence.
ACCESSION   DBNHBY010000332 DBNHBY010000000
VERSION     DBNHBY010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_831011"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="AC16HB_08215"
     CDS             complement(<1..1196)
                     /locus_tag="AC16HB_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5606816.1"
                     /translation="MPTSSHSKLSTTFDALARSSARRLHHRPRAPYVVIAMIALLAAG
                     LFVAILAEDRQLQREAMHRDIDSGALQLGARLSTLAETLSSAAREIGNGSLGEKRFAA
                     IAQDLVNKPEIDSIEYVDATGRPIWVTLPSTSSNPPNSLAPSTEADSAVDPQLAPVLR
                     QASRGYDPIFRALPAGSRGSPLVLAVPVFADRQFAGAVIARIDPLDLIIKGVANETLE
                     RYRLTLALGDRALASTSSSLPPPQALTYSATLPPLPVDFRLQAIAFRLQSRFTTSALL
                     WVVLGLTVAVMGALAALARFTTRQQRIDRALLAETTLRRAMEDSLATGLAVIDPQGTI
                     RYVNRTFSQLTGFDERELHACPPPYPFWPPDQRAANEALLHEMLAGKAPSGGFEMLVR
                     RRDGTH"
     gene            1450..>1784
                     /gene="aceE"
                     /locus_tag="AC16HB_08220"
     CDS             1450..>1784
                     /gene="aceE"
                     /locus_tag="AC16HB_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665688.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR5606817.1"
                     /translation="MSAVLKVDAANDPDALETQEWLEALDAVLEREGPHRAHYLLERL
                     IDKARRSGAYIPFSPNTAYINTIPPHREEHSPGDYAIEERIRSYIRWNAMATVVRANR
                     GEGDLGGHIA"
BASE COUNT          308 a          589 c          614 g          273 t
ORIGIN      
        1 aagtgcgtgc cgtcccggcg ccggaccagc atctcgaagc cgcccgacgg cgccttgccg
       61 gcgagcattt cgtgcagcag cgcttcgttc gcagcgcgct ggtcgggcgg ccagaacgga
      121 taaggcggcg ggcaggcgtg cagttccctt tcgtcgaacc cggtcaactg gctgaaggtg
      181 cggttcacgt aacggatcgt tccctgcgga tcgatcacgg ccagcccggt ggcgagcgaa
      241 tcctccatcg cgcgccgcag ggtggtttcg gccagcaacg cgcgatcgat ccgctgctgg
      301 cgcgtcgtga accgggccag cgcggccagc gcgcccatga ccgcgaccgt cagtccgagc
      361 accacccaca gcagcgcgct ggtggtgaac cgcgactgca gtcggaacgc gatcgcctgc
      421 aggcggaagt cgaccggcag cggcggcagt gtcgcgctgt aggtcagcgc ctgcggcggc
      481 ggcaggctcg acgacgtcga tgccagcgcg cggtcaccca gcgccagcgt caggcgatag
      541 cgctcgagcg tctcgttcgc cacgcccttg atgatcagat cgagcgggtc gatgcgcgca
      601 atgacggctc ccgcaaactg ccggtcggcg aagaccggca ccgcgagcac cagcggcgag
      661 ccgcgcgacc ccgcgggcag cgccctgaag atggggtcat agccccggct tgcctgcctg
      721 agaaccgggg caagctgcgg gtcgacggcc gagtccgctt cagtggacgg cgcgagcgag
      781 ttcggtggat tcgacgaggt cgagggcagc gtcacccaga tcggccgccc ggtcgcgtcc
      841 acgtactcga tgctgtcgat ctccggcttg ttcaccaggt cctgcgcgat ggccgcgaac
      901 cgcttctctc ccaggctgcc gttgccgatt tcgcgcgcgg ccgacgacag cgtctccgcc
      961 agcgtcgaca gccgtgcgcc cagttgcagc gcgcccgagt cgatgtcccg gtgcatcgct
     1021 tcgcgctgca gttgccggtc ttcggccagg atggcgacga acagcccggc ggcgagcagc
     1081 gcaatcatcg cgatcacgac ataaggcgca cgcgggcggt gatgcaggcg gcgtgccgaa
     1141 cttcttgcga gcgcgtcgaa tgtggtggag agcttgctgt gtgaggaggt gggcatcggc
     1201 accgtgaatg ggtcgtcgaa gcgcgcgcgg cgcgggcata ttatgggtgg cgccgatgct
     1261 gcggcccgcc gccacacatc ggtgccgcca agccgcatcg acttgtgggt aatcggaggt
     1321 tgttggggag aatttcatat aaagaaattc gacatcacta tttgaaaagc gatcgaacgc
     1381 tggtagagtg cccgcctgcg cgccgccgca gcggcgcatg cgtcacgaga acgattcaag
     1441 gagaccgtca tgtccgccgt actgaaagtc gacgccgcca acgatcccga cgcgctcgag
     1501 acccaggaat ggctcgaggc gctcgacgcg gtgctcgaac gcgaaggtcc gcaccgcgcg
     1561 cattacctgc tcgaaaggct gatcgacaag gcgcgccgct cgggcgcgta catcccgttc
     1621 tcgccgaaca cggcgtacat caacacgatc ccaccgcatc gcgaagagca ttcgccgggc
     1681 gactacgcga tcgaggagcg aatccgctcc tatatccgct ggaatgccat ggcgaccgtc
     1741 gtgcgcgcga atcggggcga aggcgatctg ggcggccaca tcgc
//
LOCUS       DBNHBY010000333         3155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_695969, whole genome shotgun sequence.
ACCESSION   DBNHBY010000333 DBNHBY010000000
VERSION     DBNHBY010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_695969"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC16HB_08225"
     CDS             complement(<1..349)
                     /locus_tag="AC16HB_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5606818.1"
                     /translation="MQVQSMHFKSRAAMKLADARLQKNLANVRDKFTRKRALAIAELD
                     DFEAIRAAAVERRDRALEDLDAWIEQFERTAIARGATVLFAQSHAEAADLIIDIARRH
                     GVRKVTKSKSMVSE"
     gene            complement(349..1074)
                     /locus_tag="AC16HB_08230"
     CDS             complement(349..1074)
                     /locus_tag="AC16HB_08230"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5606819.1"
                     /translation="MRVGLFVTCLVDLVRPEIGFSVVKLLERAGCAVHVPPTQTCCGQ
                     PGYNSGDAASARGLALKVLREFESFDHVVVPSGSCGAMIKVDYVDLFADDPDLKPRFD
                     ALAAKTFELTDFLVNVVRIDSLESAFAGSVTYHDSCGGLRYLGVKAQPRALLAKVPGV
                     RLTEMKDCEQCCGFGGTFAMKYGDISGAIVDDKCRNIRATGTDAVALGDLGCMLNIEG
                     RLRRIGDEKTRVLHIAQILAGDA"
     gene            1225..2046
                     /locus_tag="AC16HB_08235"
     CDS             1225..2046
                     /locus_tag="AC16HB_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5606820.1"
                     /translation="MRTALPAATSRPNREKTAIQVIERMMNLLDALAAHSDPVSLKEL
                     ARATGLHPSTAHRILNDMVVGRFVDRGEQPGSYRLGMRLLELGNLVKGRLSVREAALE
                     PMRDLHRRTGQTVNLSVRQGDEIVYVERSYSERSGMQVVRAIGGRAPLHLTSSGKLFL
                     AYDDPRVVRSYATRTGLAGHTRNSITELAKLERELSLVRARGFARDNEELEPGVRCIA
                     AAVRDDSGRIVAGLSVSAPSERMQDEWIDELRTTASKISARLGHSGSGAEAAETR"
     gene            complement(2050..3042)
                     /locus_tag="AC16HB_08240"
     CDS             complement(2050..3042)
                     /locus_tag="AC16HB_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5606821.1"
                     /translation="MSMRRFFFTVVAVAGLAIASLASAADSAQTAKSKSARTASADKS
                     AKAKAAKSKATRKRVAERRPLRPSEGQIAGLHRTGDPLALKSSVALVVDQDTDEVLFE
                     KNTTAVLPIASITKLMTALVTVEANLPLEEVIEVERSDRTIDRMRSWLNPGVRITRAQ
                     ALHLALMSSENHAAQLLGRTYPGGLDAFVQAMNAKAQLLGMSDSHFVDPTGLSQENRS
                     SPNDLVKLVKAAYEHQTIREYSVSSEMMLPVGRRTVRYANTNRLTASPGWDIGLQKTG
                     YISAAGRCLVMQAVIEGQRVVMVLLDSVGKYSRIGDAKRIRDWLEARKAQGAER"
BASE COUNT          565 a         1043 c          972 g          575 t
ORIGIN      
        1 cctccgaaac catcgacttc gacttggtga cctttcggac gccgtgccgg cgtgcgatgt
       61 cgatgatcag gtcggccgcc tccgcatgtg actgggcaaa cagcaccgtg gcgccacgtg
      121 cgattgcggt gcgctcgaac tgctcgatcc aggcgtcgag gtcctcgagg gcgcggtcgc
      181 ggcgttcgac tgcggccgcg cggatcgcct cgaagtcgtc cagttccgcg attgccagcg
      241 cccgcttgcg cgtgaacttg tcgcgcacgt tcgcaaggtt cttctggagt cgcgcgtcgg
      301 cgagcttcat cgccgcccgc gacttgaagt gcatcgactg cacctgcatc aggcgtcccc
      361 cgccaggatt tgcgcgatgt gcagcacgcg cgtcttctcg tcgccgatgc gccgtagccg
      421 tccctcgata ttcagcatgc agccgaggtc gccgagcgcc accgcatcgg tgccggtggc
      481 ccggatgttg cggcacttgt cgtcgacgat cgcccccgag atgtcgccgt acttcatcgc
      541 gaaggtgccg ccgaagccgc aacactgctc gcaatccttc atctcagtca gtctcacgcc
      601 cggcaccttg gcgagcaagg cacgtggctg cgccttgacg ccgagatagc gcagcccgcc
      661 gcacgaatcg tgatacgtga ccgagcccgc gaaggccgat tccagcgagt cgatcctgac
      721 gacgttgacg agaaagtcgg tcagttcgaa ggtcttcgcc gccaaggcat cgaagcgcgg
      781 cttgaggtcc gggtcatccg cgaacaggtc gacataatcg actttgatca tcgcgccgca
      841 cgatccggaa ggcacgacga cgtggtcgaa cgattcgaac tcccggagca ccttcagggc
      901 caggccgcgc gccgaggccg catcgcccga gttgtagccg ggctgtccgc agcaggtctg
      961 cgtcggcggg acgtgcactg cgcagcccgc gcgctccagg agcttgacca ccgagaaccc
     1021 gatctcaggt cgcaccagat cgaccaagca ggtcacgaac agcccaacgc gcatctttcg
     1081 ttcttatccc ggagcgcggc gattcgccaa gttgaaagtc attcgaccgg acacgtacct
     1141 gccggccgac tcgcggccgg cacgagtcga cccgtttttc gcatcatgat ataagcttca
     1201 ccaagtgaat tttcgcaatg caccatgcga accgcacttc ctgccgcaac cagccgaccg
     1261 aaccgcgaga agaccgcgat ccaggtcatc gaacgcatga tgaacctgct ggacgcactc
     1321 gccgcgcact cggacccggt tagtctgaag gaactcgcgc gcgctaccgg attgcacccc
     1381 tccaccgcgc atcgcatctt aaatgacatg gtggttggcc ggttcgtcga tcggggcgaa
     1441 cagccgggca gttatcgact cggcatgcga ttgctcgaac tcggcaacct cgtcaaggga
     1501 cgactgtcgg tcagggaagc tgcgctggag ccaatgcgcg atctgcatcg gcgcacgggc
     1561 cagacggtca acctgtcggt gcgacaaggc gacgagattg tctatgtcga acggtcgtac
     1621 agcgagcgct cgggcatgca ggtcgtgcgc gcgatcggcg gtcgcgcgcc gttgcatctg
     1681 acttcaagcg ggaagctttt ccttgcctac gacgatccgc gcgtggtccg ttcctatgcc
     1741 acgcgcacgg gcctggccgg acacactcgc aacagcatca ccgaactggc caagctcgag
     1801 cgcgaattgt cgctggtccg ggcgcgcggg ttcgcgcgcg acaacgagga actggagccg
     1861 ggtgtgcgct gcatcgcggc cgcagtgcgc gacgactcgg gacgcatcgt cgccggtctg
     1921 tccgtatcag cgccgtcgga acgaatgcag gacgagtgga tagatgagct gcggactacg
     1981 gcgtcgaaga tctccgctcg gctcggccac agcggttcag gcgccgaagc agccgaaacg
     2041 cggtgatctt cagcgctcgg cgccctgcgc cttgcgcgct tcgagccagt cgcgaatgcg
     2101 cttggcgtcg ccaatcctcg agtacttgcc caccgaatcc agcaggacca tcacgacgcg
     2161 ctgtccttcg atcaccgcct gcatcacgag gcagcgaccg gccgcggaga tgtatccggt
     2221 cttctgcaaa ccgatatccc atccggggct ggcagtcagg cgattggtat tggcgtagcg
     2281 caccgtgcgc cgaccgaccg gcagcatcat ctcgctgctg accgaatact cccgaatcgt
     2341 ctggtgctcg tacgcagcct tgacgagctt tacgagatcg ttaggactgg aacggttctc
     2401 ttgcgaaagc ccggtcgggt cgacgaagtg cgagtcagac attccgagaa gttgcgcctt
     2461 cgcgttcatc gcctgaacga aggcgtccag tccgccggga tacgtgcgac ctagcagctg
     2521 tgcggcatgg ttttccgacg acatcagcgc caggtgcagc gcctgtgcgc gcgtgatcct
     2581 gacacccgga ttcagccacg agcgcatgcg gtcgatcgtg cgatccgagc gctcgacctc
     2641 gatcacctcc tccagaggca ggttcgcttc gaccgtcacg agcgccgtca tcagctttgt
     2701 gatcgacgcg atcggcagca ccgcggtggt gttcttctcg aagaggactt cgtccgtatc
     2761 ctggtcgacg accagcgcga ccgacgactt cagtgccaga gggtcaccgg tgcgatgcag
     2821 gccggcgatc tgcccttcac tcgggcgcag cggccgccgc tccgccacgc gcttgcgtgt
     2881 cgccttggac ttagccgctt ttgcctttgc agacttgtcg gccgatgccg tgcgcgcgga
     2941 cttcgatttc gccgtctgcg ccgaatcggc cgcggatgca agcgatgcaa tcgccagtcc
     3001 ggcaacggcc acaacggtga agaagaacct gcgcatgctc atttcctacc ctcaaagcgc
     3061 gatacacgcg tacttatcgc ggatttccat gctcgggaaa atcccggcac ggccattctt
     3121 tggactaatg caatcggcgg cgcaggcccg gttcg
//
LOCUS       DBNHBY010000334         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1643797, whole genome shotgun sequence.
ACCESSION   DBNHBY010000334 DBNHBY010000000
VERSION     DBNHBY010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1643797"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..328
                     /locus_tag="AC16HB_08245"
     CDS             38..328
                     /locus_tag="AC16HB_08245"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR5606822.1"
                     /translation="MLFMVIEHFRDNDMLPVYRRVRDAGRGLPDGLRYVESWVEPNFG
                     RCFQLMECDDARLLQQWALHWRGCGVTFEFVPVVPSAATREIVAPRLDLPPA"
     gene            442..1764
                     /gene="aptA"
                     /locus_tag="AC16HB_08250"
     CDS             442..1764
                     /gene="aptA"
                     /locus_tag="AC16HB_08250"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR5606823.1"
                     /translation="MGHAEPKVAASLQPYWMPFSPNKEFKAEPRMFARAKGMYFYTPD
                     GREVIDASAGLFCVAAGHCREEIAHAVYEQLQTLDFTAPFLRAHPKAFALAERVAAYT
                     PPGLNRVFFTNSGSEAVDTAMKIALAYHRVKGQPQRQLFIARERAYHGVNMGGVSLAG
                     MVNNRRAYGVGLAGVYHMRHTALPENKFVRGQPDKGAELAEDLLRFCNLYGGENIAAV
                     FVEPAAGSFGCLPPPKGYLNRLREICDQHGILLVFDEVITGWGRMGANFGAQAFGVTP
                     DLLTMAKAITNGAQPMGAVAARQGIYDAITDAGPKQGIEFFHGYTYSAHPAACAAGLA
                     MMDIFAKERLIDRAAAMSPKFIDAIHSLRDCAIVTDIRSIGMMAAVDVAVDGAPGARG
                     HEAQKRLYDAGLNLKSTGDALIVAPPLVVEDKHIDEIVSKLRGVLTSL"
BASE COUNT          323 a          637 c          662 g          304 t
ORIGIN      
        1 atcggtccga tccaatcatc gataccggag gtctgccatg ctgttcatgg tcatcgagca
       61 ttttcgcgac aacgacatgt tgccggtcta tcgccgcgtg cgcgatgccg ggcgcggcct
      121 gcccgacggg ctgcgctacg tggagagctg ggtcgaaccg aacttcggcc gctgtttcca
      181 gctgatggag tgcgacgatg cccggctgct gcagcaatgg gcgctgcatt ggcgcggctg
      241 cggcgtgacg ttcgagttcg tgccggtcgt ccccagcgcg gcgacgcgcg agatcgtcgc
      301 gccacggctc gacctgccgc ccgcgtgatg gcgccggacc gcggcgttcg atggagccag
      361 ggcgccgatc gaccccgatg aactatccga ccgagcggca attaagtgac aatccgcctg
      421 caccgcaccg gaggagccac catgggacac gccgaaccga aagtcgccgc atcgttgcag
      481 ccgtactgga tgccgttttc gccaaacaag gagttcaagg ccgagccgcg catgttcgcg
      541 cgcgccaagg gcatgtactt ctacacgccc gacgggcgcg aggtgatcga tgcgtcggcc
      601 ggactgttct gcgtcgcggc cggtcattgc cgcgaggaga tcgcgcacgc ggtgtacgag
      661 cagttgcaga cgctcgactt caccgcgccg tttctgcgcg cgcacccgaa ggccttcgcg
      721 ctggccgagc gcgtcgccgc gtacacgccg ccggggctga accgcgtgtt cttcaccaac
      781 tcgggctcgg aagcggtcga caccgcgatg aagatcgcgc tcgcgtatca ccgcgtcaaa
      841 gggcagccgc agcggcagtt gttcatcgcg cgcgagcgcg cctaccacgg cgtcaacatg
      901 ggcggcgtgt cgctcgcggg catggtcaac aaccgccgcg cctacggcgt ggggctggcg
      961 ggcgtctacc acatgcggca cacggcgctg ccggagaaca agttcgtcag gggccagccg
     1021 gataagggcg ccgagctcgc cgaagacctg ctgcggttct gcaacctgta cggcggcgag
     1081 aacatcgccg cggtgttcgt cgaaccggcg gcgggctcgt tcgggtgcct gccgccgcca
     1141 aagggatacc tgaaccgcct gcgcgagatc tgcgatcagc acggcatcct gctggtgttc
     1201 gacgaggtga tcaccggctg gggccgcatg ggtgccaact tcggcgcgca ggcgttcggc
     1261 gtcacgccgg acctgctgac gatggccaag gcgatcacca acggcgcgca gccgatgggt
     1321 gccgtcgcgg ccaggcaggg catctacgac gcgatcacgg atgctggtcc gaaacagggc
     1381 atcgagttct tccacggtta cacgtactcg gcgcacccgg cagcgtgcgc ggcggggctg
     1441 gcgatgatgg acatcttcgc caaggagcgg ctgatcgacc gcgcagccgc gatgagtccg
     1501 aagttcatcg acgcgatcca ttcgctgcgc gattgcgcga tcgtcaccga catccgctcg
     1561 atcggcatga tggcggcggt cgacgtcgcg gtcgacggcg ctcccggcgc acgcggccac
     1621 gaagcgcaga agcgcctgta cgacgcgggg ctgaatctga agagcacggg cgatgcgctg
     1681 atcgtcgcgc cgccgctcgt tgtcgaagac aagcacatcg acgagatcgt gagcaagctg
     1741 cgcggggtgc tgacgtcgct gtagccgacc cttgcgcggc gaccgagaca ccgttcaacc
     1801 tgatcctcga agcgcggcgt tgagcggccc gcggggactg cgccgcgtcc ccgcggtgcc
     1861 ggctacatcc ggcccttcca cggcacgagc ttgtgctcga tccagcgcat gaacagatcg
     1921 aacagg
//
LOCUS       DBNHBY010000335         1768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1643819, whole genome shotgun sequence.
ACCESSION   DBNHBY010000335 DBNHBY010000000
VERSION     DBNHBY010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1643819"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="AC16HB_08255"
     CDS             <1..1157
                     /locus_tag="AC16HB_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR5606824.1"
                     /translation="EPLPEDILREINASVWTIGYTGQSPERLKAHMRNMNVFDVRTLR
                     AKGGIDKETGYSLDGDYFGLPWPCYGTPQLKHPGSPNLYDTSKHVMDGGGNFRANFGV
                     ERDGVSLLAGDGSHSKGADLTTGYPEFDHLLLKKLGWWDELTDEEKKAAEGKNWKTDP
                     SGGIIRVTMKNHGCHPFGNAKARAVVWNFPDPIPQHREPLYSIRPDLVAKYPTHDDKR
                     AFWRLPTLYKTVQEKNKDIGKTYPLIMTSGRLVEYEGGGEETRSNPYLAELQQEMFVE
                     INPKAANDRGVRDGDWVWVKTPSMAAFPDFKGLRVRAQVTERVNAETVFLPFHFSGRW
                     GGNDLLQYYPEGAHPFVRGEAVNTATTYGYDSVTMMQETKTTVCQVEKAA"
     gene            1170..>1768
                     /gene="fdh3B"
                     /locus_tag="AC16HB_08260"
     CDS             1170..>1768
                     /gene="fdh3B"
                     /locus_tag="AC16HB_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HIR5606825.1"
                     /translation="MARMKFICDAERCIECNGCVTACKAEHDIPWGVNRRRVVTLNDG
                     VPGERSISVACMHCSDAPCMAVCPVDCFYRTDDGVVLHDKDVCIGCGYCSYACPFGAP
                     QFPGNGAFGMRGKMDKCTFCAGGPEANGSQAEFEKYGRNRLAEGKLPACAEMCSTKAL
                     LGGDGDVVADIFRSRVLVRGKGSEVWGWGTAYGKATGGST"
BASE COUNT          353 a          528 c          609 g          278 t
ORIGIN      
        1 acgagccgtt gccggaggac atcctgcgcg agatcaacgc cagcgtctgg accatcggct
       61 acacgggcca gagccccgag cgcctgaagg cgcacatgcg caacatgaac gtgttcgacg
      121 tcaggacact gcgcgccaag ggcggaatcg acaaggaaac cggctattcg ctcgatggcg
      181 attacttcgg gctgccgtgg ccgtgctacg ggacgccgca actgaagcat ccgggctctc
      241 ccaacctgta cgacacgtcg aagcacgtga tggacggcgg cggcaatttc cgcgcgaact
      301 tcggcgtcga gcgcgacggc gtgtcgttgc tcgcgggcga cggctcgcac agcaagggcg
      361 cggacctgac caccgggtac ccggagttcg atcacctgct gctgaagaag ctcggctggt
      421 gggacgaact gacagacgaa gagaagaaag cagccgaggg caagaactgg aagaccgatc
      481 cctcgggcgg aatcatccgc gtgactatga agaaccacgg ctgccatccg ttcggcaacg
      541 cgaaggcgcg cgcggtggtg tggaacttcc cggacccgat cccgcagcat cgcgaaccgc
      601 tgtattcgat ccgaccggat ctggtcgcca agtatccgac gcacgatgac aagagggcgt
      661 tctggcgcct gccgacgctg tacaagacgg tgcaggagaa gaacaaggac atcggcaaga
      721 cctatccgtt gatcatgacg tcgggccgcc tcgtcgaata cgaaggcggc ggcgaagaga
      781 cgcgctccaa tccgtacctt gccgagctgc agcaggagat gttcgtcgag atcaatccga
      841 aggcggccaa cgatcgcggc gtccgggatg gcgactgggt gtgggtgaag acgccgtcga
      901 tggcggcgtt tccggacttc aagggcctgc gcgtgcgcgc gcaggtgact gagcgggtca
      961 atgccgagac ggtcttcctg ccgttccatt tctccgggcg ctggggcggc aacgaccttc
     1021 tgcagtacta cccggaaggc gcgcacccgt tcgtacgcgg cgaggcggtc aacacggcca
     1081 ccacctacgg atacgacagc gtgacgatga tgcaggagac gaagacgacg gtctgccagg
     1141 tcgagaaggc cgcgtaagga gaaaccgaca tggcgcgaat gaaattcatt tgtgacgccg
     1201 aacgctgcat cgagtgcaac ggctgcgtga cggcgtgcaa ggcggagcac gacattccat
     1261 ggggcgtcaa tcgccgtcgc gtcgtcacgc tgaacgacgg cgtcccgggc gagcggtcga
     1321 tttcggtggc gtgcatgcac tgctcggacg caccctgcat ggccgtgtgt ccggtcgatt
     1381 gcttctaccg taccgacgac ggcgtggtgc tgcacgacaa ggatgtatgc atcggctgcg
     1441 ggtattgcag ctatgcgtgt ccgttcggcg cgccgcagtt tccgggcaac ggtgcgttcg
     1501 gcatgcgcgg caagatggac aagtgcacgt tctgcgccgg cggccccgaa gccaacggat
     1561 cgcaggccga gttcgagaaa tacggccgca atcgcctggc cgagggcaaa ttgccggcgt
     1621 gcgccgagat gtgctcgacc aaggcactgc tcggcggcga cggcgacgtg gtcgccgaca
     1681 tcttccgcag ccgcgtgctt gtccgcggca aggggagcga agtgtggggc tggggcactg
     1741 cttacggcaa agcgacgggc ggttcgac
//
LOCUS       DBNHBY010000336         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1643869, whole genome shotgun sequence.
ACCESSION   DBNHBY010000336 DBNHBY010000000
VERSION     DBNHBY010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1643869"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16HB_08265"
     CDS             complement(<1..311)
                     /locus_tag="AC16HB_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR5606826.1"
                     /translation="MTQASPVPVTVLTGFLGAGKTTLLNRVLGEEHGHRIAVIENEFG
                     PEGVDNELLVTDTSEQIVEMNNGCICCTVRGDLVRILNDLKAKRERKELAFERVIIET
                     TG"
     gene            complement(369..1568)
                     /locus_tag="AC16HB_08270"
     CDS             complement(369..1568)
                     /locus_tag="AC16HB_08270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR5606827.1"
                     /translation="MKTVTLRVGKERSLRRRHPWIYDTAIAGVHGKPTAGETVAVRGA
                     DGEFLAWAAYSPQSTIRARCWSFVESDRIDAAWLGARVEAAVRRRTPLAAESNALRLV
                     FGEADGLPGFIADRYADQLVVQFQAAGVEAQRESLLDALVQATRCKDVYERSDSATRQ
                     REGLAASSGVMRGAEPPPRIEIGEHGLPFLVDVRRGHKTGYYIDQRPNRLLARRLAER
                     ARATLGRDIRALNCFCYTGGFSIALAAGGARAVVSIDSSNEALELARANAALNDQTGH
                     EWRQADVFDALRALRDAGEVFDLIVLDPPKFASSHHHVDRAARAYKDISLNALRLLAP
                     GGFLLTFSCSGAIGVDLFQKIVAGAVIDARIDAQMLARLAAGEDHPLLMTHPEGEYLK
                     GLLLQRV"
     gene            complement(1582..>2014)
                     /locus_tag="AC16HB_08275"
     CDS             complement(1582..>2014)
                     /locus_tag="AC16HB_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606828.1"
                     /translation="PNNPLSLLWTALNRLHLGQPERALPAIDEALAVSPRDGLVHIFH
                     LQRAQALFDLRRFDESVLSARRSIAARDDSMNARLMLAAALQRSGNAAAAQLALAEVA
                     ALRPGLTVERVALESMAVNADGEAMRARYFDALRDAGLPAR"
BASE COUNT          331 a          712 c          654 g          317 t
ORIGIN      
        1 cccgtggtct cgatgatcac gcgctcgaac gcgagttcct tgcgctcgcg cttcgccttc
       61 aggtcgttca ggatccgcac caggtcgccg cgcacggtgc agcagatgca gccgttgttc
      121 atctcgacga tctgctcgct cgtatcggtg acgagcagtt cgttgtcgac accctcgggt
      181 ccgaactcgt tctcgatcac cgcgatccgg tggccgtgct cttcgccgag cacgcgattg
      241 agcagcgtgg tcttgccggc acccaggaag ccggtcagga ccgtgactgg caccggtgat
      301 gcttgagtca tcccgcactc ctgaaaaggg gcggaattct aatgaaatcg gcccgccagc
      361 gcaggcgctc agacccgctg cagcagcaag cccttcaggt attcgccctc cgggtgcgtc
      421 atcagcagcg ggtggtcctc gccggccgcc agccgcgcga gcatctgcgc gtcgatgcgc
      481 gcgtcgatca ccgcgccggc gacgattttc tggaacaggt cgacgccgat cgcacccgag
      541 cacgagaacg tcagcaggaa gccgcccggc gcgagcagcc gcagcgcatt gaggctgatg
      601 tccttgtacg cgcgcgccgc gcgatcgacg tggtggtggc tcgacgcaaa cttgggcgga
      661 tcgagcacga tcagatcgaa gacttcgccg gcgtcgcgca gcgcgcgcag cgcatcgaac
      721 acatcggcct ggcgccactc gtgccccgtc tgatcgttca aagcggcgtt ggcgcgtgcc
      781 agttccagcg cttcgttcga cgagtcgatc gacaccaccg cgcgcgcgcc gcccgccgcc
      841 agcgcaatgg agaagccgcc cgtgtagcag aagcagttca gcgcgcggat gtcgcggccg
      901 agcgttgcac gcgcgcgctc ggccagccgg cgcgcgagca gacggttcgg ccgctggtcg
      961 atgtaatagc ccgtcttgtg gccacgccgt acgtcgacca ggaagggcag cccgtgttcg
     1021 ccgatctcga tccgcggcgg cggctccgcg ccgcgcatga caccgctgga cgcggcgagg
     1081 ccctcgcgct ggcgcgttgc gctgtcggag cgctcgtata cgtccttgca gcgcgtcgcc
     1141 tgcaccagcg cgtcgagcag cgattcgcgc tgcgcttcga cgcccgcggc ctggaactgg
     1201 acgacgagtt gatccgcgta gcgatcggcg atgaagcccg gcaggccatc ggcctcgcca
     1261 aacaccagcc gcagcgcatt cgactcggca gcgagcggcg tgcgccgccg aaccgctgct
     1321 tcgacgcgcg cgcccagcca ggccgcatcg atgcggtcgg actcgacgaa gctccagcag
     1381 cgcgcgcgga tcgtcgattg cggcgaatac gcggcccagg cgaggaattc gccgtccgcc
     1441 cctcgcaccg ccacggtttc gcccgctgtc ggcttgccgt gcacccctgc aatcgcggtg
     1501 tcgtagatcc agggatggcg gcgccgcaac gaccgctcct tgccgacgcg cagcgtgacc
     1561 gtcttcatgg gttcgctgcg atcagcgtgc gggcaacccg gcgtctcgca gggcgtcaaa
     1621 gtaacgcgcc cgcatcgcct cgccatcggc gttcactgcc atcgactcca gcgccacgcg
     1681 ttctactgtc agacccggcc gcaatgcggc cacttcggcc aacgccaact gtgccgcggc
     1741 cgcattgccg ctacgctgca acgccgccgc caacatcagg cgcgcattca tagagtcatc
     1801 gcgcgcagcg atcgatcggc gcgccgacag cacggattca tcgaaccgcc gcaaatcaaa
     1861 cagcgcctgg gcgcgttgga ggtgaaaaat gtggacgagt ccatcgcgcg gactgaccgc
     1921 caacgcctca tcgatcgcag gcagcgcgcg ctccggctgt ccgagatgga gacgattcaa
     1981 cgcggtccag agcagcgaga gcggattgtt cggc
//
LOCUS       DBNHBY010000337         7567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1440145, whole genome shotgun sequence.
ACCESSION   DBNHBY010000337 DBNHBY010000000
VERSION     DBNHBY010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1440145"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16HB_08280"
     CDS             complement(<1..356)
                     /locus_tag="AC16HB_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR5606829.1"
                     /translation="MTSDRDPIVAIATPPGRGGIGIVRVSGADLAPLLAGVLGSAAEA
                     RLAPRHATLARFLDAQGATIDEGIALLFPAPHSYTGETVLELHGHGGPVVMELLLARS
                     LEAGRAIGLRVAEPGE"
     gene            397..684
                     /locus_tag="AC16HB_08285"
     CDS             397..684
                     /locus_tag="AC16HB_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606830.1"
                     /translation="MPGEDPLLKIARPLAGLLIAVVISMLIAALIGGALPLDYAGRAW
                     VYTALMCYVIAGAVVVFRLAAAGETQPLSPARILKWTLSLWAWPALLLARR"
     gene            complement(695..2455)
                     /gene="yidC"
                     /locus_tag="AC16HB_08290"
     CDS             complement(695..2455)
                     /gene="yidC"
                     /locus_tag="AC16HB_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR5606831.1"
                     /translation="MKDGMDIQRTVLWIIFGMSLLFLYDNWQRDQGRPSLFGAPPVPT
                     QAAKGTPETTAGAPAPAKDSSVPSAAPVQPAALPSVVAPPPVDSAPTAAAQRVRIETD
                     TVIAEIDPNGAVLSHLELRKQKVAPDWTAAGLMSLVTGKKQDREANVVLLEVSANRVY
                     TAQTGLVGAAGSNLPNHRTSFAPLDGPRTLQEGKDALEVKFAAESGGVKLVKTYTFKR
                     GRYDIDVRHEVVNTGTDAVTASLYLQLLRDGNKPAGESGPYSTFTGPAVYTDAEKFHK
                     VEFSGIEKNKASYPKSSKDGWVAMIQHYFVSAWVPPEGAQREIYSRLVDKNLYAIGSI
                     LPLPSIAPGATATSQETLYVGPQDQNALKAIAPGLDLVVDYGWLTVIAKPLFWLLQFF
                     HGLVGNWGWAIILLTICVKAAFYPLSATSYKSMAKMKEVTPRLMKLREQYGDDKQKLN
                     MAMMELYKTEKINPLGGCLPIVVQIPVFIALYWVLLASVEMRNSPWILWVKDLATPDA
                     WFILPLVMMATMWVQYKLNPTPPDPVQAKVMAAMPFIFGVMFFLFPAGLVLYWVVNNV
                     LSIVQQWYVTKQIAKAKAVG"
     gene            complement(2466..2765)
                     /gene="yidD"
                     /locus_tag="AC16HB_08295"
     CDS             complement(2466..2765)
                     /gene="yidD"
                     /locus_tag="AC16HB_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR5606832.1"
                     /translation="MKHVLILAIRIYQYLLSPWVGGSCRFWPTCSEYAREAIERHGAL
                     RGGWMTVARLARCHPRGGGGVDPVPEQFRWRCWCGSEARHDTSTNNEAARVATKT"
     gene            complement(2762..3166)
                     /locus_tag="AC16HB_08300"
     CDS             complement(2762..3166)
                     /locus_tag="AC16HB_08300"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5606833.1"
                     /translation="MPRAARLRAARDFEACRAPQFRAGIRWVGVSARIADAAVEAPTA
                     QVRFGVTAGKRMARKAVHRNLVKRILREAARHGRPQLIESARGRRVDVVLRLKAAFPT
                     TAEMTLATFKRTLRADADALLLRLAQHLAAQP"
     gene            complement(3204..3344)
                     /gene="rpmH"
                     /locus_tag="AC16HB_08305"
     CDS             complement(3204..3344)
                     /gene="rpmH"
                     /locus_tag="AC16HB_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5606834.1"
                     /translation="MATKRTFQPSVVKRKRTHGFLVRMKSRGGRAVLRARRARGRQRL
                     AP"
     gene            3934..5289
                     /gene="dnaA"
                     /locus_tag="AC16HB_08310"
     CDS             3934..5289
                     /gene="dnaA"
                     /locus_tag="AC16HB_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5606835.1"
                     /translation="MVAIWQDCSRVLERELTPQQYSAWIKPLRPIEYDAVRGRLRIGA
                     PNRFKLDAIRTQFAARIQAVATNVTQRPVDIVFEVDASNDAAAREMPAEADSTEAVLE
                     ERTAAVDQFERARLNPQLTFDTFVTGKANQLARAAAMQVAENPGGSYNPLFLYGGVGL
                     GKTHLVHAVGNALLARKPTARVRYIHAEQYVSDVVRAYQRKAFDEFKRYYHSLELLLI
                     DDIQFFAGKNRTQEEFFYAFEALVAGRKQIIITSDTYPKELKEFEDRLVSRFASGLTV
                     AIEPPELEMRVAILLKKAEAAGLAVPEDVAFFIAKNLRSNVRELEGALARLRAFSSFT
                     DRALTVEVAREALKDVIGASTGQITVELIQKMVADYYKIKVSEMYGKRRPSSIALPRQ
                     IAMYLAKEMTQKSLPEIGERFGGRDHTTVLHAVRKIGLQRSSNTELNHALHVLEQGLK
                     G"
     gene            5522..6625
                     /gene="dnaN"
                     /locus_tag="AC16HB_08315"
     CDS             5522..6625
                     /gene="dnaN"
                     /locus_tag="AC16HB_08315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5606836.1"
                     /translation="MQLVKATRDALLKPLQVVSGIVERRQTLPILANVLVRKDGDRVS
                     FTATDLEIQIQTEASIGAGKEDAAATVSARKLVDILRALPEADVQLSLATRKLTVQSG
                     RSRFNLQTLPAEEFPTVAQAEFTADFALPAATLKYLLSMVHFAMAAQDIRYYLNGMLL
                     VVDGNLVRAVATDGHRLALADVQKEGDATRKIEAIIPRKTILEMSRLLPDSDAPVRVQ
                     MAANQVKFTFDSIELISKLVEGKFPDYSKVIPSGNSRLFKVRREELMAALSRAAILTT
                     DKFKGVRWVLAPGSLKITSTNADQEDAQEELEIGYDGDGLDIGFNVNYLLDVLANLKN
                     EEVKFALGDALGSALITMPESDKFKYVVMPMRI"
     gene            6675..>7567
                     /locus_tag="AC16HB_08320"
     CDS             6675..>7567
                     /locus_tag="AC16HB_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5606837.1"
                     /translation="MNLPPLGGDGSAANDYGAASIQILEGLEAVRKRPGMYIGDTHDG
                     TGLHHMVFEVVDNSIDEALAGYCDDIVVTIHTDNSISVIDNGRGIPTGVKLDDKHDPK
                     RSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRRDRQVHYLEF
                     RQGATQNRRIETQDDVTVSPMHVLGPTDRRGTEVHFLPDDEIFGKVDFHYEVLSKRLR
                     ELSFLNNGVRIRLIDQRIGKEEDFAFSGGVKGFVEYINKSKTVLHNNIFHCVGDAAGI
                     SVEVAMQWNDGYSESLLAFTN"
BASE COUNT         1326 a         2558 c         2452 g         1231 t
ORIGIN      
        1 aactcgccgg gctccgccac gcgcaggccg atcgcgcggc ccgcctccag actgcgcgca
       61 agcagcagct ccatcacgac cgggccccca tgaccgtgca gttcgagcac cgtttcgccg
      121 gtgtacgagt gcggtgccgg gaagagcagc gcgatgcctt cgtcgatcgt ggcaccctgc
      181 gcgtcgagaa agcgcgccag cgtcgcgtgg cgcggcgcga gtcgtgcctc ggccgcggac
      241 ccgagcacgc ccgcgagcag cggcgccaga tcggcgcccg agacccgcac gatgccgatc
      301 cctccgcgcc ccggcggcgt ggcgattgca acgatggggt cgcggtcgga ggtcatgcga
      361 gcaacctagc acagcgcggc tccgtacact gcgcgcatgc cgggtgaaga tcctctgctg
      421 aagatcgcgc ggccgctggc cggcctgctg atcgcggtcg tgatctcgat gctgatcgcg
      481 gcgctgatcg gcggcgcgct tccgctcgac tatgcgggtc gcgcctgggt ctacacggcc
      541 ctgatgtgct acgtgatcgc cggcgcggtg gtcgtgttca ggctcgccgc cgccggcgaa
      601 acgcaaccgc tatcgccggc gcggattctg aagtggaccc tgtcgctgtg ggcgtggccc
      661 gcgctgctgc tggcgcggcg ctgaactgcg ccgctcagcc gaccgccttc gccttcgcga
      721 tctgcttggt cacgtaccac tgctgcacga tcgacagcac gttgttgacg acccagtaga
      781 gcaccagccc ggcagggaac aggaagaaca tcacgccgaa gatgaacggc atcgcggcca
      841 tcaccttggc ctgcaccggg tcgggcggcg tcgggttcag cttgtactgc acccacatgg
      901 tcgccatcat caccagcggc aggatgaacc acgcgtcggg cgttgcaagg tccttgaccc
      961 acaggatcca cggcgaattg cgcatctcca cgctcgccag cagcacccag tacagcgcga
     1021 tgaacacggg gatctgcacc acgatcggca ggcagccgcc gaggggattg atcttctccg
     1081 tcttgtacag ttccatcatc gccatgttca gcttctgctt gtcgtcgccg tactgctcgc
     1141 gcagcttcat caggcgcggc gtgacctcct tcatcttcgc catcgacttg tagctggtgg
     1201 cggacagcgg ataaaaggcc gccttcacgc agatcgtcag caggatgatc gcccagcccc
     1261 agttgccgac caggccgtgg aagaactgca gcagccagaa cagcggcttg gcgatgacgg
     1321 tgagccagcc gtagtcgacc acgagatcca gccctggcgc gatcgccttc agcgcgttct
     1381 ggtcctgcgg cccgacgtac agcgtctcct gcgatgtcgc ggtcgcgccc ggcgcgatcg
     1441 acggcagcgg caggatcgag ccgatcgcgt acaggttctt gtcgaccagt cgcgagtaga
     1501 tctcgcgctg cgcgccttcc ggcggcaccc acgccgacac gaagtagtgc tggatcatcg
     1561 ccacccagcc gtccttgctg ctcttggggt aggacgcctt gttcttctcg atgcccgaga
     1621 actcgacctt gtgaaacttc tccgcgtcgg tgtagacggc cggtccggtg aaggtcgaat
     1681 acgggcccga ttcgcccgcg ggcttgttgc cgtcgcgcaa tagctgcagg tatagcgacg
     1741 ccgtgaccgc gtcggtgccg gtgttgacga cctcgtgcct gacgtcgatg tcgtagcggc
     1801 cgcgcttgaa cgtataggtc ttgaccagct tcacgccgcc cgactcggcg gcaaacttca
     1861 cctcgagcgc gtccttgccc tcctgcagcg tgcgcggccc gtcgagcggc gcaaaggacg
     1921 tgcggtgatt gggcaggttg ctgcccgccg cgcccaccag tccggtctgc gcggtgtaga
     1981 cgcgattcgc gctcacttcg agcagcacca cgttggcctc gcggtcctgc ttcttgccgg
     2041 tgaccagcga catcagccca gccgcggtcc agtcgggcgc gaccttctgc ttgcgcaact
     2101 ccaggtgcga cagcactgcg ccgttcggat cgatctccgc gatcaccgtg tcagtctcga
     2161 ttcgcacgcg ctgtgccgcg gcggtcggcg cgctgtcgac cggcggcggt gcgacgacgc
     2221 tgggcagcgc ggcgggttgc acgggcgccg cgctcggcac cgaggaatcc ttggcgggcg
     2281 cgggtgcgcc cgccgtggtc tccggcgtgc ctttggccgc ctgcgtcggc accggcggcg
     2341 cgccgaacag cgacggccgg ccctgatcgc gctgccagtt gtcgtacagg aacagcagcg
     2401 acatcccgaa gatgatccac aggaccgtgc gctggatatc catgccgtct ttcatcgcag
     2461 tcttctcagg tcttggtggc gacgcgcgcc gcctcgttgt tcgtcgacgt gtcgtgtcgc
     2521 gcttcgctgc cgcaccagca gcgccagcgg aattgttcgg gcaccgggtc gaccccgccg
     2581 ccgccgcgcg ggtgacagcg cgccagacgc gcaaccgtca tccagccgcc gcgcagcgcg
     2641 ccgtggcgct cgatcgcttc gcgcgcgtat tccgagcagg tcggccagaa gcggcaactg
     2701 ccgccgaccc agggcgacag caggtactgg tagatacgga tcgcgaggat caggacgtgc
     2761 ttcatggctg cgccgccagg tgctgcgcca gccgcagcag cagcgcatcg gcgtcggcgc
     2821 gcagcgtgcg cttgaacgtc gcaagcgtca tttcggccgt tgtcggaaac gccgccttca
     2881 gccgcagcac gacgtcgacc cgccgcccgc gggcggactc gatcagttgc ggccgcccgt
     2941 gccgggccgc ctcgcgcagg atccgcttca ccaggtttct gtgcaccgct ttgcgcgcca
     3001 tccgcttacc cgccgtcacg ccaaagcgca cctgtgcagt cggtgcttcg accgcggcgt
     3061 cggcgatgcg tgccgaaacg ccgacccagc gtatcccggc gcgaaactgc ggcgcacggc
     3121 acgcttcgaa gtcacgcgcc gcacgcagcc gcgccgcgcg cggcaggccg agtgcgcgtg
     3181 tgtcggacgt gcgccgctcc gcctcagggc gcgagccgct ggcggccgcg cgcgcggcgc
     3241 gcccgcagca ccgcgcggcc gccgcgcgac ttcatccgca ccaggaatcc gtgcgtgcgc
     3301 tttcgcttga cgacggaggg ttgaaacgta cgtttggtgg ccatgatggt gattcctcaa
     3361 cgaatccgtt cagcggatgc gggtggcgcg ggtcgagccc gatcgggtcg cgcgctgcgg
     3421 tggccagcgc gaactgactc ggtaccgcgg cgcggcaaaa gcccgtaatt agacgcgaga
     3481 tcgcgcgcca gcgtcaaacc gattcccgga cgcggcgatc cacagccttt tgtggacaga
     3541 ttgacgactt atcccccgaa aatcccggct tatccacaag ctttgcagcc gacaaaaatt
     3601 ctttgccacc gattgttcaa gccgaccggg attgtcagtg cttgctttct ctgaacgcct
     3661 ttacttatca ggaattccca tcgcccgcta cgcgtgcatc ggtccgccgc gctgtggata
     3721 accacggcgg cgagggctaa gattcgcgct cccttgtgaa tcgcccgccc cggaccgccc
     3781 atcacggcag tacgggaccg gacgccgagc aagcgggtcg gcggcatggg gatgccgcca
     3841 caagaacaac gagagaaagc ggctcgaaca aagacacccg ccagaccggc atcggcattg
     3901 gcggacactc acacggggcg aagaaggaat ccgatggtcg ctatctggca agactgctcg
     3961 cgcgtgctcg agcgcgagtt gactccgcag cagtattccg cctggatcaa acccctgaga
     4021 ccgatcgaat acgacgccgt gcgcggacgg ctgcgcatcg gcgcgccgaa ccggttcaag
     4081 ctcgacgcga tccgcaccca gttcgccgcg cgcatccagg ccgtggcgac caatgtgacg
     4141 cagcggccgg tcgacatcgt gttcgaggtc gatgcaagca acgacgcggc cgcgcgcgaa
     4201 atgcccgccg aggccgacag caccgaggcg gtcttggagg agcgcaccgc cgctgtcgac
     4261 cagttcgagc gcgcgcggct caacccgcag ctcaccttcg ataccttcgt caccggcaag
     4321 gccaaccagc tcgcgcgcgc cgcggcgatg caggtcgccg agaatcccgg tggttcgtac
     4381 aacccgctgt tcctgtacgg cggcgtgggt ctgggcaaga cgcacctggt gcacgcggtt
     4441 ggcaacgcac ttttggcgcg caagccgacg gcgcgcgtgc gctacatcca cgccgagcag
     4501 tacgtgagcg acgtggtgcg cgcctaccag cgcaaggcgt tcgacgagtt caagcgctac
     4561 taccactcgc tcgaactgct gctgatcgac gacatccagt tcttcgccgg caagaaccgc
     4621 acgcaggagg agttcttcta cgcgttcgag gcgctggtcg ccggccgcaa gcagatcatc
     4681 atcacgtcgg acacctatcc gaaggagctg aaggaattcg aagaccgcct ggtgtcgcgc
     4741 ttcgcatcgg gcctgacggt cgcgatcgag ccgccggaac tcgagatgcg ggtggcgatc
     4801 ctgctgaaga aggccgaggc ggcgggcctg gcagtgcccg aggacgtggc gttcttcatc
     4861 gcgaagaacc tgcgcagcaa cgtgcgcgaa ctcgaaggcg cgctggccag gctgcgcgcc
     4921 ttttcctcgt tcaccgaccg cgcgctgacg gtcgaagtcg cgcgcgaggc cttgaaggac
     4981 gtgatcggcg cctcgaccgg ccagatcacg gtcgagttga tccagaagat ggtcgccgac
     5041 tactacaaga tcaaggtttc cgagatgtac ggcaagcggc gcccgtcctc gatcgcgctg
     5101 ccgcgccaga tcgcgatgta cctcgccaag gaaatgacgc agaagagcct gccggagatc
     5161 ggggaacgct tcggcggccg cgaccacacc accgtgctgc acgcggtgcg caagatcggg
     5221 ctgcagcgct cgtccaacac ggaactgaac cacgcactgc acgtgctcga gcaggggctg
     5281 aagggctgat tcctgcctgt ggagagccgc tggactgact gtggatggct gtggacagcc
     5341 tgcgcccgcc gcgcgaccca tgcgcagttg ttcgcgcccg tccacaccga ttccagcgcc
     5401 gcaggctgca gttgcccaca gcatttgaga tcatccaagt tcccgctgcg gcagcgtttt
     5461 gccgtgttgt acccaggaat ggcgccgatc tattagttag ttctacgaag aggaagtaca
     5521 aatgcaactt gtaaaagcca cgcgcgacgc gttgctcaaa ccgctgcagg ttgtcagtgg
     5581 gatcgtcgag cggcggcaga cgctgccgat cctggccaac gtcctcgtgc gcaaggacgg
     5641 cgatcgcgtg tcgttcacgg cgaccgatct cgagatccag atccagaccg aggccagcat
     5701 cggcgccggc aaggaggacg ccgcggcgac ggtatcggcg cgcaagctcg tcgacatcct
     5761 gcgcgcgctg cccgaagccg acgtgcagct gtccctggcc acgcgcaagc tcaccgtgca
     5821 gtcgggccgc agccgcttca acctgcagac gctgccggcc gaggagttcc cgaccgtcgc
     5881 gcaggccgag ttcacggccg acttcgcgct gcccgcggcg acgctgaagt acctgctgtc
     5941 gatggtccac ttcgcgatgg ccgcgcagga catccgctac tacttgaacg gcatgctgct
     6001 ggtcgtcgac ggcaacctcg tgcgcgcggt cgccaccgac ggtcatcggc tcgcgctcgc
     6061 cgacgtgcag aaggagggcg acgccacgcg caagatcgag gcgatcatcc cgcgcaagac
     6121 gatcctcgag atgtcgcggc tgctgcccga ctcggacgcg ccggtgcggg tgcagatggc
     6181 cgccaatcag gtcaagttca cgttcgactc gatcgaactc atctccaagc tggtcgaagg
     6241 caagttcccc gactactcga aggtgatccc cagcggcaac tcgcgcctgt tcaaggtgcg
     6301 gcgcgaggaa ctgatggcgg cactgtcgcg cgcggcgatc ctgaccaccg acaagttcaa
     6361 gggcgtacgc tgggtcctgg cgccgggcag cctcaagatc acgtcgacca acgccgacca
     6421 ggaagacgcg caggaggaac tcgagatcgg ctacgacggc gacgggctcg atatcggctt
     6481 caatgtcaac tatctgctcg acgtgctggc gaatctcaag aacgaggaag tgaagttcgc
     6541 gctcggcgac gccctcggca gcgcgctgat cacgatgccg gagtccgaca agttcaagta
     6601 cgtcgtcatg ccgatgcgaa tctgatgtgt cgaatggaag ccaattgatg tccgcagtcc
     6661 cgaaccagcc tcctatgaat cttccgccgt tgggcggcga cggcagtgcc gccaacgact
     6721 acggcgccgc cagtatccag atcctggaag ggctggaggc ggtgcgcaag cgcccaggca
     6781 tgtacatcgg cgacacgcac gacggcaccg ggctgcacca catggtgttc gaggtcgtcg
     6841 acaactcgat cgacgaggcg ttggccggtt actgcgacga catcgtcgtc acgatccaca
     6901 ccgacaactc gatctcggtg atcgacaacg gccgcggcat tccgaccggc gtgaagctcg
     6961 acgacaagca cgatccgaag cgctccgccg ccgagatcgc gctcaccgaa ctgcacgccg
     7021 gcggcaagtt caaccagaac agctacaagg tgtcgggcgg gctgcacggc gtgggcgtgt
     7081 cgtgcgtgaa cgcgctgtcc aagtggctgc ggctgaccgt gcggcgcgac cgccaggtgc
     7141 attacctcga gttccgccag ggcgcgacac agaaccgccg catcgagaca caggacgacg
     7201 tgacggtgtc gccgatgcat gttctgggcc cgaccgaccg gcgcggtacc gaggtgcact
     7261 tcctgccgga cgacgagatc ttcggcaagg tcgacttcca ctacgaggta ctgtcgaaac
     7321 ggctgcgcga gctcagcttt ctcaacaacg gcgtgcgcat ccgcctgatc gaccagcgca
     7381 tcggcaagga agaggacttc gcgttctccg gcggcgtcaa gggcttcgtc gagtacatca
     7441 acaagtccaa gacggtcctg cacaacaaca tcttccactg cgtcggcgac gcggccggca
     7501 tcagcgtcga agtcgcgatg cagtggaacg acggctacag cgaaagcctg ctcgcgttca
     7561 ccaacaa
//
LOCUS       DBNHBY010000338         3572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1711784, whole genome shotgun sequence.
ACCESSION   DBNHBY010000338 DBNHBY010000000
VERSION     DBNHBY010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1711784"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="AC16HB_08325"
     CDS             complement(<1..1327)
                     /locus_tag="AC16HB_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5606838.1"
                     /translation="MHSTYRYPRFEYVKPPELKTGSRRRYPVVIVGAGPVGLTAALDC
                     RLHGIPVVVLDDDDTVSVGSRGLCYAKRALEILDRVGVGDAVVGKGVGWNVGRTFFRD
                     EEVYRFDLQPEPDHKRPGMINLQQYYLEEYLVGRCNESGVAIRWKHRVVSVKPADDRV
                     ALEVETADGVYHLETDWLIAADGARSSIRTLLGLDIEGRIFMDRFLIADVVMKADFPA
                     ERWFWFDPPFHPNQSVLLHREADHVWRIDFQLGWDVDPEEEKKPEKVIPRIQAMLGDR
                     ACNSGREGATVPDFELEWVSVYTFQCRRMDRFNHGRVLFVGDAAHQLSPFGARGANSG
                     MQDSDNLVWKLKLVLDGKAPPRLLDTYSEERVYAADENILNSTRSTDFITPKSATSRT
                     FRNAVLQLARRHAFARKLVNSGRLSVPAFYVDSSLNTPDEDTFSSHLVPG"
     gene            complement(1411..2373)
                     /locus_tag="AC16HB_08330"
     CDS             complement(1411..2373)
                     /locus_tag="AC16HB_08330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5606839.1"
                     /translation="MEHAMSKKFASHADLEEKKVSFDKLSDNAYAYTAEGDPNTGVVI
                     GDDAVLVADTQATPVMAQDVIRRIRAVTDKPIKYVVLTHYHAVRVLGASAYGAQQIIA
                     SQDTYDLIVERGEADMKSEIERFPRLFRAVESVPGLTWPTLAFKGEMTLWLGKLEVKL
                     MQLGRGHTKGDTVVWLPQQKILFSGDLVEFDATPYGGDAYFEDWPRTLDAIARLAPEK
                     LVPGRGPALTTPERVQAGLASTRAFISDLYASVKQGAAAGKDLRAVYRETYDRLKPKY
                     GHWVIFDHCMPFDVSRAYDLATKYPDPRIWTAQRDMEMWEALEG"
     gene            2472..3221
                     /locus_tag="AC16HB_08335"
     CDS             2472..3221
                     /locus_tag="AC16HB_08335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606840.1"
                     /translation="MTQTLKVALVTGAGTGIGRAAALALARDGYAVVLAGRARDKLEA
                     TAGRTSARTLVVPTDVCDPQSVQALFARVQKEFGRLDLLFNNAGVGAPAVPLEELSYA
                     QLRAVIDTNLTGCFLCAQEAIRMMKAQTPRGGRIINNGSISAHAPRPNSAPYTATKHA
                     VTGLTKSIALDGRPFDIACGQIDIGNAATEMTERMAAGVMQANGQVAIEPRMNVDHVG
                     QAISYMANLPLESNVLFMTVMATNMPFVGRG"
     gene            complement(3226..>3572)
                     /locus_tag="AC16HB_08340"
     CDS             complement(3226..>3572)
                     /locus_tag="AC16HB_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5606841.1"
                     /translation="AKIEAFPGIARAELIRAARDLYDFDNEYTAPVHGFRDTDDYWAR
                     ASGKPWLGGVRVPHLVLNARNDPFVPAPSLPSTHEVSRAVRLEQPEHGGHIGFAGDFL
                     ARRLLEFFERGV"
BASE COUNT          579 a         1214 c         1194 g          585 t
ORIGIN      
        1 cgcccggaac cagatgcgac gagaaggtgt cttcgtccgg ggtgttcagg gacgaatcga
       61 cgtagaacgc gggcaccgac agtcgccccg agttcaccag cttgcgcgcg aacgcgtgcc
      121 tgcgcgcaag ctgcagcacg gcgttgcgga acgtccggct ggtcgcgctc ttgggcgtga
      181 tgaaatcggt cgagcgggtc gagttcagga tgttctcgtc ggcggcgtac acgcgctcct
      241 cggagtacgt gtcgagcagc cgcggtggcg ccttgccgtc gagcacgagc ttgagtttcc
      301 ataccaggtt gtcgctgtcc tgcatccccg agttggcgcc gcgcgcgccg aacggcgaca
      361 gctggtgcgc ggcatcgccg acgaacagaa cgcgcccgtg attgaagcga tccatccggc
      421 ggcactggaa cgtatagacg ctgacccatt ccagctcgaa gtcgggaacg gtggcccctt
      481 cccttccgct gttgcacgct cgatcgccga gcatggcctg gatgcgcggg atgaccttct
      541 cgggcttctt ctcttcttcc ggatcaacgt cccagccgag ctggaagtcg atgcgccaca
      601 cgtgatccgc ctcccggtgc aacagcaccg actggttcgg atgaaacggc gggtcgaacc
      661 agaaccagcg ttcggccggg aagtcggcct tcatcaccac gtcggcgatc aggaagcggt
      721 ccatgaagat ccggccctcg atgtcgagcc cgagcagggt gcggatcgaa ctgcgcgcgc
      781 cgtcggcggc gatcagccag tcggtctcca ggtgatagac gccgtcggcc gtctccacct
      841 caagcgcgac gcgatcgtcg gcgggcttga ccgacaccac cctgtgcttc cagcggatcg
      901 cgaccccgct ctcgttgcaa cggccgacga gatactcttc gaggtagtac tgctgcaggt
      961 tgatcatgcc gggccgcttg tggtccggct ccggctgcag gtcgaagcga tacacctcct
     1021 cgtcgcggaa gaacgtgcgg ccgacgttcc agcccacgcc cttgccgacc accgcgtcgc
     1081 cgacgccgac gcggtcgagg atctcgagcg cacgcttcgc gtagcagagc ccgcgcgatc
     1141 cgaccgacac ggtgtcgtcg tcgtcgagca cgacgaccgg gataccgtgc agccggcagt
     1201 cgagcgcggc ggtgagtccc accggaccgg cgccgacgat gacgaccgga tagcgccgtc
     1261 tggacccggt cttgagttcg ggcggcttga cgtactcgaa gcgcggatag cgataggtgc
     1321 tgtgcacgtc agtaccgccc ggccgtccga aggtactgac acgccccctc ggggggcagc
     1381 gagcgaagcg agcgtggggg ctgcttcatc ctagccctcc agggcttccc acatctccat
     1441 gtcgcgctgc gcggtccaga tgcgcgggtc ggggtacttg gtcgccaggt cgtaagcgcg
     1501 cgacacgtcg aacggcatgc agtggtcgaa gatcacccag tggccgtact tcggcttgag
     1561 ccggtcgtag gtttcgcgat agaccgcgcg cagatccttg ccggccgccg cgccctgctt
     1621 gaccgacgcg tacaggtcgc tgatgaaggc gcgcgtgctc gcgaggcctg cctgcacgcg
     1681 ctccggcgtg gtcagcgcag ggccgcgccc ggggacgagc ttctccggcg caagccgcgc
     1741 gatcgcgtcg agcgtccgcg gccaatcctc gaagtacgcg tcgccgccgt acggcgtggc
     1801 gtcgaactcc acgaggtcgc cggagaacag gatcttctgc tgcggcagcc atacgacggt
     1861 gtcgcccttg gtgtgcccgc gcccaagctg catcagcttc acttccagct tgccgagcca
     1921 gagcgtcatc tcgcccttga aggcgagcgt cggccaggtc agccccggca ccgactcgac
     1981 cgcgcggaac aagcgcggaa agcgctcgat ctcgctcttc atgtccgcct cgccgcgctc
     2041 gacgatcagg tcgtaggtgt cctggctggc gatgatctgc tgcgctccgt aggcagacgc
     2101 accgagcacg cgcaccgcgt ggtagtgggt cagtaccacg tacttgatcg gcttgtcggt
     2161 gaccgcgcgg atgcggcgga tcacgtcctg cgccatcacc ggcgtggcct gcgtgtcggc
     2221 gaccagcacg gcatcgtcgc cgatcacgac gcccgtattg gggtcgccct cggccgtgta
     2281 cgcgtacgcg ttgtcggaca gcttgtcgaa gctgaccttc ttttcctcga ggtcggcatg
     2341 cgatgcgaat ttcttgctca tggcgtgctc catttccgcg gtcggtcgtc gcccaggata
     2401 ggcgaactcg ccgcagtcgt gcccggcgcg gtagccttcg aggctcgtgc atcgacggta
     2461 cgagggccat catgacgcag acactgaagg tcgcactggt gacgggcgcc ggtaccggca
     2521 tcggacgcgc cgcggcgctg gcgctggcgc gcgacggcta cgccgtggta ctggccgggc
     2581 gcgcgcgcga caagctcgaa gccaccgccg gccggacgag cgcgcgcacg ctcgtcgtac
     2641 cgaccgatgt ctgcgacccg cagtcggtgc aggcgctgtt cgcgcgcgtg cagaaggaat
     2701 tcggccgcct cgacctgttg ttcaacaacg ccggcgtcgg cgcaccggcg gtgccgctgg
     2761 aggaactgtc ctacgcgcag ttgcgcgcgg tgatcgacac caatctcacc ggctgctttc
     2821 tgtgcgcgca ggaggcgatc cgcatgatga aggcacagac gccgcgcggc ggccgcatca
     2881 tcaacaacgg ctcgatctcc gcgcacgccc cgcgcccaaa ctccgccccc tacaccgcaa
     2941 ccaagcacgc ggtcacgggg ctcaccaagt cgatcgcgct cgacggccgc ccgttcgata
     3001 tcgcctgcgg ccagatcgac atcggcaacg ccgccaccga gatgaccgag cgcatggcgg
     3061 ccggcgtgat gcaggccaac ggccaggtcg ccatcgaacc gcgcatgaac gtcgatcacg
     3121 tcggccaggc gatctcgtac atggcgaacc tgccgctgga gtcgaacgtg ctgttcatga
     3181 cggtgatggc gacgaacatg ccgttcgtgg gacgggggta gtccgctaga cgccgcgctc
     3241 gaagaattcg agcagccgcc gcgcgaggaa gtcccccgcg aaaccgatgt gcccgccatg
     3301 ctccggctgc tcgagccgca ccgcgcgcga cacttcgtgc gtgctgggca agctcggcgc
     3361 gggaacgaat ggatcgttgc gcgcattcag caccaggtgc ggaacgcgga cgccgccgag
     3421 ccacggcttg cccgacgcac gcgcccagta gtcgtcggtg tcgcgaaagc cgtgcaccgg
     3481 tgcggtgtac tcgttgtcga agtcgtacag atcgcgcgcg gcgcgaatca gttcggcgcg
     3541 cgcgatgccg ggaaacgcct cgatcttcgc ca
//
LOCUS       DBNHBY010000339         2890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_290741, whole genome shotgun sequence.
ACCESSION   DBNHBY010000339 DBNHBY010000000
VERSION     DBNHBY010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_290741"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="AC16HB_08345"
     CDS             complement(<1..1452)
                     /locus_tag="AC16HB_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5606842.1"
                     /translation="MDRKRLLLLVVIIAAIAAFFIFDLKQYFSLDYFQASRARIEAFR
                     DANYLQTAAAFFAIYVAVAALSLPGAAVMTLAAGAIFGLAPGVVLVSFASAIGATLAF
                     LISRLILGEWVQQRFADKLAPINAGIEKEGAFYLFALRLVPLFPFFVINLVMGLTRIR
                     TTTFYWVSQLGMLAGTFVYVYAGTQLGQFRISVGLVFAFVLLGIFPLVAKKVLDAIKA
                     RKVYAKWADKRPPKYDYNMVVIGAGSAGLVSAYIAAATKARVALIEKHRMGGDCLNTG
                     CVPSKALIRSAKLLSHIARSKEFGVATASASFDFADVMERVAKVVRTVEPHDSVDRYS
                     KLGVECVTGAAKITSPWTVEVTTAAGRKMLTTKNIVIAAGARPFVPPIPGIETVGYLT
                     SDTVWNLRELPKRLVVLGGGPIGSELTQAFARFGSAVTQVEMLPRIMIREDPEVSELV
                     KKQFESEGIRVLTEHKAKQFVVENGEKIAIVEHK"
     gene            complement(1538..2503)
                     /locus_tag="AC16HB_08350"
     CDS             complement(1538..2503)
                     /locus_tag="AC16HB_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5606843.1"
                     /translation="MSLSPGRSCPPHYGYSPRVFARAAPFAEETIYVIGGLYGNGFAL
                     DAIERLAAREPVAPRLVFNGDFHWFDADPALFTEIERRVTAEPNVALRGNVETELAGD
                     DPAAGCGCAYPESVPDDDVERSNAILERLRAVARHCRRDDPVGELPMHTVVAVGGARI
                     AIVHGDAWSLAGWRFASEALHDPAHDRQLTTAFEQAAVDGFVCSHTCAPVLKVLGDRF
                     VINNGAAGMANFAGTTHGVLTRVSVHAPQGEIARARLYAIRRANVYIDALRIDFDCAA
                     WIERFDSLWDAESPAAVSYRRRIVAGPRFTVAQALGATRNASACA"
     gene            complement(2553..>2890)
                     /locus_tag="AC16HB_08355"
     CDS             complement(2553..>2890)
                     /locus_tag="AC16HB_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HIR5606844.1"
                     /translation="ANMPIARFGSTLISKGQFRDYMNTLRQAHRTENVEHVMCRSLVS
                     VDWQGYLYDCDFNQMLGLHLHEGGRGHGRIHLDDWLEMDLSSNPIVVADHCYGCTAGQ
                     GSSCGGALA"
BASE COUNT          495 a          984 c          946 g          465 t
ORIGIN      
        1 cttgtgctcg acgatggcga tcttctcgcc attttcgacg acgaactgct tggccttgtg
       61 ctcggtcagc acgcggatgc cctcggactc gaactgcttc ttcaccagct cggagacctc
      121 ggggtcctcg cggatcatga tccgcggcag catctcgacc tgcgtcaccg cactgccaaa
      181 gcgcgcgaac gcctgcgtga gttcgctgcc gatcggaccg ccaccgagca cgacgaggcg
      241 tttcggcagt tcgcgcaggt tccacacggt gtccgacgtc aggtagccaa ccgtctcgat
      301 gcccgggatc ggcggcacga acggccgtgc gccggcggcg atcacgatgt ttttcgtcgt
      361 cagcatcttg cggccggcgg cggtcgtcac ctcgaccgtc cacggcgacg tgatcttcgc
      421 ggctccggtc acgcactcca cgccgagctt gctgtagcgg tcgaccgaat cgtgcggctc
      481 gaccgtcctg acgaccttgg ccacgcgctc catcacgtcg gcaaagtcga agctcgccga
      541 tgccgtggcg acgccgaact ccttgctgcg cgcgatgtgc gacagcagct tggccgaccg
      601 gatcagcgcc ttgctcggca cgcagccggt gttcaggcag tcgccgccca tccggtgctt
      661 ctcgatcagc gcgaccctgg ccttggtcgc cgccgcgatg taggccgaca ccagtccggc
      721 gctgccggcg ccgatcacga ccatgttgta gtcatacttt ggggggcgct tgtcggccca
      781 tttcgcgtag accttgcgcg ccttgatcgc gtcgagcact ttcttggcga ccagcggaaa
      841 gatgccgagc agcacgaacg cgaagaccag gccgaccgag atgcggaact ggccaagctg
      901 ggtacccgcg tatacgtaca cgaacgtgcc ggccagcatg ccaagctgac tgacccagta
      961 gaacgtggtg gtgcggatcc tggtcagccc catcacgagg ttgatcacga agaacggaaa
     1021 cagcggtacc agccgcagcg cgaacagata gaacgcgcct tctttctcga tgccggcgtt
     1081 gatcggcgcc agcttgtcgg cgaatctctg ctgcacccac tcgccgagga tcagccgcga
     1141 gatcaggaac gccagcgtgg cgccgatcgc ggacgcgaac gacaccagca ccacgccggg
     1201 ggcaagcccg aagatcgcgc cggccgcgag tgtcatgacc gccgcgcccg gcagcgacag
     1261 cgccgccacc gcgacataga tggcgaagaa ggcggccgcc gtctgcaggt agttggcgtc
     1321 gcggaacgcc tcgatccgtg cgcggctcgc ctggaaatag tcgaggctga agtactgctt
     1381 caggtcgaaa atgaaaaacg ccgcgatcgc cgcgatgatc accaccagca gcaacagtcg
     1441 cttcctgtcc atgattcccc caacgattcg tgacgcacgg gcaagtgccc gtgccgcgat
     1501 cagtcggaaa gcagcgttgg ctcttacgag gataagacta cgcgcacgcg gaagcgttgc
     1561 gcgttgcgcc cagtgcctgc gcgacggtga agcgcggacc cgcgacgatg cggcgccgat
     1621 acgacaccgc ggccggtgat tcggcatccc acaggctgtc gaagcgctcg atccaggcgg
     1681 cgcagtcgaa gtcgatgcgc agcgcgtcga tgtatacgtt ggcgcgccgt atggcgtaca
     1741 gccgcgcccg cgcgatctcg ccttgcggcg cgtgaaccga aacgcgcgtg agcacgccgt
     1801 gcgtggtgcc ggcgaaattc gccatgccgg ccgcgccgtt gttgatcacg aaccggtcgc
     1861 ccagcacctt cagcaccggc gcacaggtgt ggctgcacac gaagccgtcg accgcggcct
     1921 gctcgaaggc cgtcgtgagc tgccgatcat gggcgggatc gtgcagggcc tcggacgcga
     1981 agcgccagcc cgccagcgac caggcgtcac catgcacgat ggcgatgcgc gctccgccga
     2041 ccgcgacgac cgtgtgcatc ggcagttctc ccacggggtc gtcacggcgg cagtggcggg
     2101 caaccgcgcg cagccgctcg aggatcgcgt tgctgcgttc gacgtcatca tccggcaccg
     2161 attccggata ggcgcagccg cagcccgctg ccggatcgtc gccggccaat tcggtctcga
     2221 cgttgccgcg cagcgcgacg ttcggttccg ccgtgacgcg ccgctcgatc tccgtgaata
     2281 gcgccgggtc cgcgtcgaac cagtggaagt cgccattgaa gacgagacgt ggtgccaccg
     2341 gctcgcgcgc cgcgagccgc tcgatcgcgt cgagcgcaaa cccattgccg tacagcccgc
     2401 cgatgacgta gatcgtctcc tcggcgaacg gcgctgcgcg cgcgaagacg cgcggggagt
     2461 agccgtagtg gggcggacag gaccggcccg ggctcaacga catgatggat gctgccgctg
     2521 cccgtcgccg ggcacgcggc gcccgtcctc gatcatgcca gggcgcctcc gcacgacgag
     2581 ccctggcctg cggtgcagcc gtagcagtga tccgccacca cgatcgggtt gctcgagagg
     2641 tccatctcca gccagtcgtc gaggtggata cggccgtggc cgcggccgcc ctcgtgcagg
     2701 tgcaggccga gcatctggtt gaaatcgcag tcgtacagat agccctgcca gtcgaccgaa
     2761 acgagcgagc ggcacatcac gtgctcgacg ttttccgtcc ggtgcgcctg ccgcagcgtg
     2821 ttcatgtagt cgcggaactg tcccttgctg atcagcgtgg agccgaagcg cgcgatcggc
     2881 atgttggcca
//
LOCUS       DBNHBY010000340         1618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1711954, whole genome shotgun sequence.
ACCESSION   DBNHBY010000340 DBNHBY010000000
VERSION     DBNHBY010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1711954"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="AC16HB_08360"
     CDS             complement(<1..881)
                     /locus_tag="AC16HB_08360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5606845.1"
                     /translation="MSAFHLPPTMHVLERGWLSSNNVVFIDGAEASVVDTGYCLHAEQ
                     TAHLIDRARGDRPLTRIVNTHLHSDHAGGNARLRAQHAATVVIPPGLAAAVDAWDVEA
                     LTYAPTRQQCPRFSYDALLRAGDRLNLGAMQWEVLAAPGHDPHMVMLFNHDERLLISA
                     DALWENGFGAIFPEIEGESGFDDQRAALELVARHRPRVVIPGHGPPFGGVERALERAH
                     ARLDTLASSPERNARHVLKVLAKFWLMQVRESTRTAARSHFEGARYFQVIHRRYFADL
                     GFAAMIDRTFQELAGAGA"
     gene            complement(884..1315)
                     /locus_tag="AC16HB_08365"
     CDS             complement(884..1315)
                     /locus_tag="AC16HB_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606846.1"
                     /translation="MTARVAFDAPVSGPAYGRGFRVFATLAVLALLAFGARAVFVLLP
                     EPPSRDGWFFLGAVVFALIGSWYFMVTARTTIDAQGIRQSGLIERRTAWDEVRHARVR
                     GPGFSRRLVVRNVAGRFRYYYGGTPELAAAFERIASAYPRA"
     gene            complement(1312..>1618)
                     /locus_tag="AC16HB_08370"
     CDS             complement(1312..>1618)
                     /locus_tag="AC16HB_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5606847.1"
                     /translation="YAARSRFGALTASGTHTASLLMALTATHFSQHAQPLGLDFTLKF
                     RKAVKAGDTLALRWTVTRIEFKPSLNGELVALVGEAVNQHGEVALSATSLLLVSDKG"
BASE COUNT          271 a          577 c          539 g          231 t
ORIGIN      
        1 gcaccggcgc ccgcaagctc ctggaaggtg cgatcgatca tcgccgcaaa gcccaggtcc
       61 gcaaagtacc gccgatggat gacttgaaaa tagcgagccc cttcgaaatg cgagcgcgcc
      121 gcggtgcgtg tcgattcgcg cacctgcatc agccagaact tggcgagcac cttcagcacg
      181 tggcgtgcgt tgcgctcggg gctcgacgcc agcgtgtcga gccgcgcatg cgcgcgctcg
      241 agcgcgcgtt ccacgccgcc gaacggcggg ccgtggccgg gaatcacgac gcgtgggcga
      301 tgccgggcaa ccagttccag cgccgcccgc tggtcgtcga agcctgactc gccctcgatc
      361 tccggaaaga ttgcgccgaa gccgttctcc cacagcgcat cggccgagat caacagtcgc
      421 tcgtcgtggt tgaacagcat caccatgtgg gggtcgtgcc cgggcgcggc cagcacctcc
      481 cactgcattg cgcccaggtt cagccggtcg ccggcacgca gcagcgcgtc gtaggagaac
      541 cgcgggcact gctgccgagt cggcgcatag gtcagcgcct cgacatccca cgcatcgacg
      601 gccgccgcca gcccgggcgg gatcacgacc gtcgccgcgt gctgcgcgcg caaccgcgca
      661 ttgccaccgg cgtgatcgga gtgcaggtgc gtgttgacga tgcgcgtcaa tggtcggtcg
      721 ccgcgcgcgc ggtcgatcag gtgcgccgtc tgctccgcgt gcaggcaata gccggtgtcg
      781 accacgctcg cttcggcgcc gtcgatgaac accacgttgt tcgacgacag ccagccgcgt
      841 tcgagcacgt gcatcgtcgg cggcaggtga aacgcgctca cgctcatgca cgcggataag
      901 cgctcgcgat gcgctcgaac gccgccgcga gttccggcgt cccgccgtag tagtagcgga
      961 atcggcccgc gacgttgcgc acgaccagcc ggcgcgagaa ccccggcccg cgcacgcgcg
     1021 catgacgcac ctcgtcccac gcggtacggc gctcgatgag cccgctctgg cggatgccct
     1081 gcgcgtcgat cgtcgtgcgt gcggtgacca tgaaatacca gcttccgatc agcgcgaaca
     1141 ctaccgcgcc gaggaagaac cagccgtcgc ggctcggtgg ctccggcagc agcacgaata
     1201 ccgcgcgtgc gccgaatgcg agcagcgcca ggaccgcaag ggtggcaaac acgcggaatc
     1261 cgcgaccgta cgccggtccg ctgaccggcg catcgaacgc aacgcgcgcg gtcatccctt
     1321 gtcgctgacc agcagcagag acgttgccga cagcgcgacc tcgccgtgct ggttgaccgc
     1381 ttcgcccacc agcgccacga gttcaccatt cagcgacggc ttgaactcga tccgcgtcac
     1441 cgtccagcgc agcgccagcg tatcgccggc cttcaccgcc ttgcggaact tcagcgtgaa
     1501 gtcgagcccg agcggctgcg cgtgctgcga gaagtgggtc gccgtcagcg ccatcagcag
     1561 cgacgcggtg tgcgtgccgc tggccgtgag cgcaccgaag cgcgagcgtg cggcgtag
//
LOCUS       DBNHBY010000341        11267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1103581, whole genome shotgun sequence.
ACCESSION   DBNHBY010000341 DBNHBY010000000
VERSION     DBNHBY010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1103581"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1195)
                     /locus_tag="AC16HB_08375"
     CDS             complement(161..1195)
                     /locus_tag="AC16HB_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606848.1"
                     /translation="MSAVLKSTLAPVRGEVIVKATDLAKHFDVSPPWLNRVLERKPRA
                     ILHAVDGVSFEIRRGETLSLVGESGCGKSTVARLLVGLYQPTRGRIEFFGRDLLEMNT
                     AAGRELRQKLQMIFQDPYASLNPRWRVADIIAEPLRAHGLMQARQAIDSRVDELLTQV
                     RLAPADRDKYPHQFSGGQRQRISIARALATHPEFLVCDEPTSALDVSVQAQVLNLMKD
                     LQRELGLTYLFISHNLAVVHHISDRVGVMYLGRLVELAPRRTIFASPRHPYTRMLLDA
                     IPDLEMTGRPRTPVAGEVPNPLDPPSGCTFHPRCPHANDRCKREVPQFRQVPEGAALV
                     ACHAVEEGRI"
     gene            complement(1390..2352)
                     /locus_tag="AC16HB_08380"
                     /pseudo
     CDS             complement(1390..2352)
                     /locus_tag="AC16HB_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2361..2756)
                     /locus_tag="AC16HB_08385"
     CDS             complement(2361..2756)
                     /locus_tag="AC16HB_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606849.1"
                     /translation="MTLRPAALLASALLAGCATSDTFSYIQGQRWLKSELNTFDVLVI
                     RVDDQDYIQRGYNDPIRIDPGSHKIVVQGPPTAGFRYGEQRTLALDVKPCTRYWLEAK
                     KQNALSQDFEPRVNYAEPIAGCGAKTARY"
     gene            complement(2840..3751)
                     /locus_tag="AC16HB_08390"
     CDS             complement(2840..3751)
                     /locus_tag="AC16HB_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606850.1"
                     /translation="MQLLARWWDSDIAYSFRKSPVAMGAALVAIVLVVSAVFAHWVAP
                     LNPFDLATINLLDASKPPSWIAGGDPRFLFGTDDQGRDVFSAILFGMRISLAIGVASV
                     LLSIVLGVGLGLLSGYAGGRIDAIIMRICDVMLSFPAILVALLIDGVFRAILPREAHG
                     EVAFGVLVLAIALSGWVQYARTVRGSTMVEKNREYVQAARVIGVSPPAIMFRHVLPNV
                     LGPVLVLATVHIATAIITEATLSFLGVGVPPTTPSLGTLIRIGNEFLFSGEWWITVFP
                     GAALVLLVLSVNLLGDWLRDALNPKLR"
     gene            complement(3827..5413)
                     /locus_tag="AC16HB_08395"
     CDS             complement(3827..5413)
                     /locus_tag="AC16HB_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606851.1"
                     /translation="MKIRVLAARIALAMMAAMAVGIAPVHAKTLRMANQGDPLSLDPH
                     SLNETLQLTFLSNVYEGLVGYGKDLKIIPLLATDWKQTSPTVWRFNLRKGVKFHDGTP
                     FTADDVIFSYERAKADGSDMKAYVGSITEVRKINDHAVDFVTAAPNPILPNLFVRWLI
                     MSKKWCIDNRAERPVDKRKGVENTASFKANGTGPFRVKSRDPGNRTVLASNPSYWDKV
                     ETNIDEVVFTPIANPSTRVAALISGEIDMMEPVPVQDVARINSTPGVMVLQGPELRTI
                     FLGMDQKRDELLFSSVKGKNPFKDKRVRQAFYQAIDIEAIKNNVMRGAATPTGLMEAP
                     GVAGFPADLNKRLPYDPEGAKKLLADAGYPNGFEVGMNCPNDRYVNDAAICQAVAAML
                     ARAGVKVNLMAESKATYFPKILSRNTSFYLLGWTPSTVDSHDPLYNLMATPGAGGQGQ
                     FNLGSYSNPKLDELTTKIQSETDQARRNAMIRDAMKIHQDDVGHIPLHQQALAWGLKK
                     NLSLVQLPSNDIFVKWMVLK"
     gene            complement(5427..6638)
                     /locus_tag="AC16HB_08400"
     CDS             complement(5427..6638)
                     /locus_tag="AC16HB_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HIR5606852.1"
                     /translation="MGSYLGAALKAPVSFVAQHHDALTAIRRDLHAHPEIGFEEHRTS
                     GLVAERLHQWGIEVHTGIGRTGVVGVIRGKSEASGKSVGLRADMDALPIREENDFPHR
                     SKKQGCMHACGHDGHTTMLLGAAQYLAGTRNFDGTVYVIFQPGEEGYAGARAMIDDGL
                     FVRFPADEVYGQHNWPGLPPGHIGLNPGPMMAAADRVEITITGRGGHGAHPHLTIDPV
                     LIAAHVITAAQSIVARNVAPLEAAVVSLCAINSGNLDAFSVVPREAKISGTVRAFQHS
                     VQEQIERRLRELAEATARGFGGQASVVYQRMYPATINHKPNYEFAATVAEELIGRERV
                     ARNLPPSMGAEDFAFMLQVKPGCYARLGQGGAEGGWLHSTRYDFNDAVIPLGAGFLAA
                     LAERRMPLTTT"
     gene            complement(6638..7621)
                     /locus_tag="AC16HB_08405"
     CDS             complement(6638..7621)
                     /locus_tag="AC16HB_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606853.1"
                     /translation="MAAFVMRRLLQAVLVMLAVAFVAFLLFQYVGDPVAQMLGQDATP
                     EDRARLRADLGLDQPFFVQFVHFVANAVQGDFGISLRQGRKVSTLIQERLPATLELAL
                     AAALIAIFVGIPMGVYTALRRNSWLSQLLLAISLLGVSLPTFLIGILLILVFAVQLGV
                     LPSYGRGETVALGWWTTGLLTKKGWTHLILPSITLALFQLTLIMRLVRSEMLEVLRTD
                     YIKFARARGLTNRAVHFGHALKNTLVPVITITGLQLGGIIAFAIVTETVFQWPGMGLL
                     FIQSVQFADIPVMAAYLCLIALVFVLINLIVDLLYFAVDPRLRIDRTALAH"
     gene            7790..8269
                     /gene="msrB"
                     /locus_tag="AC16HB_08410"
     CDS             7790..8269
                     /gene="msrB"
                     /locus_tag="AC16HB_08410"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR5606854.1"
                     /translation="MMTRRTFLSGLTGLVAGADASAAAKPSPNRPDRLELPESEWKKR
                     LTPEQFDVLRREGTERAFTSPLNTEKRKGTYHCVACDLPLFASDMKYDSGTGWPSFYT
                     TLPDAFETSTDFKLLYPRTEYHCARCGGHHGHVFNDGPAPTGKRWCNNGVALKFVPV"
     gene            8280..8894
                     /gene="msrA"
                     /locus_tag="AC16HB_08415"
     CDS             8280..8894
                     /gene="msrA"
                     /locus_tag="AC16HB_08415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5606855.1"
                     /translation="MIRRLILALALSLAAGVSFAQVPAAPATAKAIFAGGCFWCVESD
                     FDKIDGVISTTSGYTGGNVPNPTYEQVSSKRTGHAEAVEVVFDPRKVSYAQLVEYFWH
                     TIDPTTKNQQFCDVGTPYRTAIFALDAEQLRIAQDSKARLEKTKPFNDPVVTEIVMAG
                     PFYPAEEYHQDYYKKNPLRYAYYRTGCGRDARLKQLWGDKAGHM"
     gene            complement(8901..>11267)
                     /locus_tag="AC16HB_08420"
     CDS             complement(8901..>11267)
                     /locus_tag="AC16HB_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606856.1"
                     /translation="SGFLLRGDGDAHEVQRVLTGAIDALPFASALRAAAKWRPTAYVL
                     HIAASVLLVVVALEFVRGSRPRARIAVALAAALALTSLFISPGLHDGLDPVYRGPWYF
                     VALQDLLQQAPVATLASTLAALLALWLLPRLPSPWTARARSAMALLALGFLGLCASGL
                     FVRGEEGGRYSAWPHSRGDLQVGWILRGTPPQAGAVAAAMVRGRAEGCRACHRDIGGF
                     DRSHRPEVLGCAACHGGDPFTLDAGRAHRDMFRVPGNLADARRTCGQSGCHVAIAPRV
                     ERSIMATMAGVVATDRRVLGDPVDPTALPPRADRLAHGVADSHLRELCVSCHLGQPKT
                     EWGAIGQESRGGGCNACHLVYGKDAAAELARYLAIEPEKRNAIPRTHPGLTVNPTNEH
                     CFGCHSRSSRISLSYEGWHEMNQAPRDATDPARLRPLDDGRTLERVVPDVHHERGLDC
                     IDCHTSNEVMGSGAVVARKSEQLRISCTDCHANRLASIDLASIDPESRTLLTLRNWTL
                     TSSQRMGAAANGEPLVNLVVDRDGRGQLRRKRTGELLPLKPPLPACTGDAGHGRLSCA
                     SCHSAWAPRCTTCHTAFDPDDVGYDNVERAWVRGTWNETAGPFEVAPPTLGVHGERGA
                     AGRIETFVPGMILTFDRNRDPGKPPDVIFRRLDSLTFMHTIRREARSCRSCHNDPIAL
                     GYGSGSLRYEIKGNVGRWTFEPKRPPSPYDGLPADAWIGFLQARSDMTSTRDDVRPFS
                     VDEQKRILRVGACLTCHAENSKVVRRALADFDAVLALRSQRCVLPTWP"
BASE COUNT         2013 a         3697 c         3662 g         1895 t
ORIGIN      
        1 gtgaccagca tgcgaccttc cggcaggaac gcgagactcc acggatgctc gagaccttcg
       61 gtcaccttga cgatgcggac cgcgtgctcg cgcgtctgca cgacctgcgc ggacggggac
      121 agcgtcatcg cggcgagcgc agccagaacc caaagggatt tcagatccgg ccttcctcga
      181 ccgcgtggca ggcaaccagc gcggcacctt ccggcacctg acggaactgc ggcacctcgc
      241 gcttgcagcg gtcgttcgcg tgcgggcagc gcggatggaa cgtgcagccg ctcggcggat
      301 cgagaggatt cggcacttcc ccggcgaccg gcgtgcgtgg gcgacccgtc atctccagat
      361 ccggaatcgc gtcgagcagc atgcgcgtgt aggggtgccg tggcgacgcg aagatagtgc
      421 gcctgggcgc gagctcgacc agccggccga ggtacatcac gccgacgcga tcggatatgt
      481 ggtgcaccac ggcgaggttg tgcgagatga atagatacgt cagcccgagt tcgcgctgca
      541 gatccttcat caggttcagc acctgtgcct gcaccgatac gtccagcgct gaggtcggtt
      601 cgtcgcagac gagaaactcc ggatgggtgg cgagcgcacg cgcgatcgag atgcgttggc
      661 gctgcccgcc ggagaactgg tggggatact tgtcgcggtc ggccggcgca agccgtactt
      721 gcgtcagcag ctcgtccacg cgcgaatcga tcgcctgtcg ggcctgcatc aggccatggg
      781 cacgcaaagg ctcggcgatg atgtccgcca cgcgccagcg cggattcagg cttgcgtatg
      841 ggtcctggaa gatcatctgc agcttctgcc gcagttcgcg gccggccgcg gtgttcatct
      901 cgagcagatc gcgaccgaag aactcgattc gtccgcgcgt gggctggtac aaacccacca
      961 gcagccgcgc gacggtcgac ttgccgcaac ctgactcgcc gaccagcgac agcgtttcgc
     1021 cacggcgaat ctcgaagctc acgccgtcga cggcgtgcag gatcgcgcgc ggcttgcgct
     1081 ccagcactcg attgagccac ggcggcgaaa cgtcgaaatg cttggcgagg tcggtcgcct
     1141 tgacgatcac ttcgccacgc acgggagcca gcgttgattt caggactgcg ctcattcttc
     1201 gtcggcgggc cggatgatcg tcttgtagtg gtcgatctgg ctggcgtcgc cgtcgaagta
     1261 ccgcgctccc ttcttcttcg tctcggccac gaccttttcg accggaagca cggtgttgaa
     1321 gtcgttgatc aggctcgcgt cgccatcgaa atacgcacct tccttgcacg ccacgcccat
     1381 cgcatcggaa tcatgcagcc agcacgcagc ttccgacgtg ccggccggca tcaggttggg
     1441 ccgcttgtgc aggcagcgat cgaacgtctt gggacagcgt ggatgaaaag cgcaaccgcg
     1501 cggaatggca gtcagccgcg gcatgctgcc ttcgatctgc gccagccgct cggcgtcctg
     1561 cgccaagctc ggaatcgacc ccatcagccc gaccgtgtac ggatgctgcg ggcgatggat
     1621 cacctcggca acgggcccga tctcggcgat ccttcctgcg tacattacgg cgacacgatc
     1681 ggccgtctcg gcgatcacgc ccatgtcgtg cgttatcaac atcaccgcgg tcttcttctc
     1741 gcggcacaag cgcttcagca gcgtgatgat ctgtgcctgg atcgacacgt cgagcgcggt
     1801 ggtcggctcg tccgcaatca cgagcttggg ctccgccgcc agcgcgagcg cgatcaccac
     1861 gcgctggcgc atgccgccgg agaattggtg cggatagtgg tcaatgcgac gctccgccgc
     1921 cgggatgccg gtagcgagca gcaggtcgat cgcgcgccgt cgcgcctgag cctgcgacac
     1981 gtgcaggtgc gtggtgatcg tttcgacgag ttgctgtccg accgtgtaca gcggattgag
     2041 cgacgtaaga ggatcctgga agatcgcccc gatctcgcgc ccgcgcacct tgcgcatctc
     2101 gtcgttcggc agtttgtcga tgcgcaggcc ggccaacttg acttcgccgc tggcgatacg
     2161 tcccggtggc tcgagcagcc cgatgatcgc cgcgccggtc agcgacttgc cggcgccaga
     2221 ttcgccgacc accccgagca cttcacctgg agcgatctcg aacgagacgt cgtcgaccgc
     2281 gaccagcgta ccgcgccggg tcggaaactc gatccgcagg tttctgactt cgagcagcgg
     2341 ggcggccgtc aatcgacctc ctagtagcgg gcggtcttcg cgccgcagcc ggcaatgggt
     2401 tcggcgtagt tcacgcgtgg ctcgaagtcc tgcgacagtg cattctgctt cttcgcttcg
     2461 agccagtaac gagtgcacgg cttgacgtcg agcgctagcg tgcgctgctc gccgtagcgg
     2521 aagccggcgg tcggcggacc ctgcacgacg atcttgtgcg accccggatc gatgcggatc
     2581 gggtcgttgt agccgcgctg gatgtagtcc tgatcgtcga ctcggatcac cagcacgtca
     2641 aaagtgttca gctcggattt gagccagcgc tgtccctgga tgtacgagaa cgtgtcgctg
     2701 gtcgcgcatc cggccagaag cgcggatgcg agcaatgcag cgggtcgcag tgtcatggca
     2761 acctccttgg gctctctgcg gtgtgtcggt caagctagca gagcaattcg acacgtgccg
     2821 taacttttcg ttgcggggac tagcgcagct tgggattcag tgcgtcgcgc aaccagtcgc
     2881 ccagcaggtt gaccgacagc accagcagca ccagcgcggc accggggaag accgtgatcc
     2941 accactcacc ggagaagaga aattcattgc cgatccggat cagcgtgccg agcgacggcg
     3001 tggtcggcgg cacacctacg ccgaggaacg agagcgtggc ttcggtgatg atcgcggtgg
     3061 caatgtggac ggtcgccagc accagcaccg ggccgagcac gttgggcagc acatggcgga
     3121 acatgatcgc cggcggggac acgccgatca cgcgcgccgc ctgcacgtac tctctgttct
     3181 tttcgaccat ggtcgagccg cggacggtgc gcgcgtattg cacccagcca gacagcgcaa
     3241 tcgccagcac cagtacgccg aacgcgactt cgccgtgcgc ctcgcgcggc agaatcgcgc
     3301 ggaagacgcc atcgatcaga agcgcgacca ggattgccgg gaatgacagc atcacgtcgc
     3361 agatgcgcat gatgatcgca tcgatgcggc cgcccgcata acccgacagc aaaccgaggc
     3421 caaccccgag gacgatcgaa agcagcaccg acgcgacgcc gatcgcgagc gaaatgcgca
     3481 tgccgaacag gatcgccgag aacacgtcgc ggccctgatc gtccgtgccg aaaaggaatc
     3541 gtggatcgcc gcccgcgatc cacgacggcg gcttggacgc atcgagcagg ttgatcgtcg
     3601 ccaggtcgaa cggattcagc ggcgcgaccc agtgcgcgaa taccgccgag acgaccagca
     3661 cgatcgcgac cagcgccgcg cccatcgcca ccggcgattt gcgaaagctg taggcaatgt
     3721 cgctgtccca ccaacgggcg agcagttgca tccttatccg tgacccattg ttcgtgatcc
     3781 gtccgtcagt ggcgtgcgac tcacccggca cggaccacgg gtcacgtcac ttcagaacca
     3841 tccacttcac gaagatgtcg ttcgacggca actgaacgag gctgaggttc ttcttcagac
     3901 cccacgccag cgcctgctgg tgcagcggga tatggccgac gtcgtcctgg tggatcttca
     3961 tcgcgtctcg aatcatcgcg ttgcgcctgg cctggtcggt ctccgactgg atcttcgtcg
     4021 tcagctcgtc gagcttcggg ttgctgtagc tgccgaggtt gaactgtccc tgcccgcccg
     4081 cccccggcgt ggccatcagg ttgtacagcg ggtcatgcga gtcgaccgtc gacggcgtcc
     4141 agccaagcag gtagaaggac gtattgcgcg acaggatctt cgggaagtag gtggccttgc
     4201 tctcggccat caggttcacc ttgacgccgg cgcgagcgag catcgccgcg accgcctggc
     4261 agatcgccgc gtcattgacg tagcgatcgt tcgggcagtt catgccgact tcgaagccat
     4321 tcgggtagcc ggcatcggcg agcagcttct tggccccctc tggatcgtat ggcagccgct
     4381 tgttcaggtc cgccggaaat cccgccacgc ccggcgcttc catcagcccg gtcggcgtgg
     4441 ccgcaccgcg catcacgttg ttcttgatcg cctcgatgtc gatggcctga tagaaggcct
     4501 gtcgcacgcg cttgtccttg aatgggttct tgcccttaac gctcgagaac agcagctcgt
     4561 cgcgcttctg gtccatgccg aggaagatcg tgcgcagctc ggggccctgc agcaccatga
     4621 cgccaggcgt cgaattgatg cgcgcgacat cctgcaccgg caccggctcc atcatgtcga
     4681 tctcgccgga aatcagcgcc gccacgcgcg tcgacgggtt ggcgatcggc gtaaagacga
     4741 cttcgtcgat gttggtctcg accttgtccc aatagctcgg attgctggcc agaacggttc
     4801 ggttgcccgg atcgcgcgac ttgacgcgga acggtccggt gccgtttgcc ttgaacgatg
     4861 cggtgttctc cacccccttg cgcttgtcga ccgggcgctc ggcgcggttg tcgatgcacc
     4921 acttcttgct catgatgagc cagcgcacga acaggttggg caggatcggg ttgggagcgg
     4981 cggtcacgaa atcgaccgcg tgatcgttga tcttccgaac ctccgtgatc gagccgacgt
     5041 aggccttcat gtcggagccg tcggccttgg cgcgctcgta cgagaagatc acgtcgtcag
     5101 cggtgaacgg cgtgccgtcg tgaaacttca cacccttgcg caggttgaat cgccacaccg
     5161 tgggcgaggt ctgcttccag tcggtggcga gcagcggaat gatcttcagg tccttgccgt
     5221 agccgaccag tccctcgtaa acattggaca ggaaggtgag ttgcagtgtc tcgttcagcg
     5281 aatgcggatc gagcgacagc ggatcgccct gattggccat gcgcagcgtc ttggcgtgca
     5341 ccggcgcgat tcccacggcc atcgcagcca tcatcgccag cgcgatgcgc gcggcaagca
     5401 cccgtatctt catgtcgaac ctcctgtcag gtcgttgtca gcggcattct gcgttcggcc
     5461 agcgcggcca gaaagccggc gccgagcgga atcacggcat cattgaaatc atatcgagtc
     5521 gagtgcagcc agcccccttc ggcgccgccc tgcccgagcc gcgcatagca gcccggcttc
     5581 acctgcagca tgaaggcgaa atcctccgcg cccatcgacg gcgggaggtt gcgcgcaacg
     5641 cgttcacgac cgatcagctc ctcggcgacc gtcgccgcga attcatagtt gggcttgtgg
     5701 ttgatcgtgg cgggatacat gcgctgatag accacgctcg cctgcccgcc gaacccgcgg
     5761 gccgtggctt cggcgagttc gcgcagccgc cgttcgatct gctcctgcac gctgtgctgg
     5821 aatgcgcgca cggtgccgga gatcttcgcc tcgcgcggca ccacgctgaa ggcgtcgaga
     5881 ttgcccgagt tgatcgcgca aagcgacacc accgcggcct cgagcggcgc gacgttgcgt
     5941 gcgacgatcg actgcgcggc ggtgataacg tgcgcggcga tcagcaccgg gtcgatcgtc
     6001 agatgtgggt gcgcgccgtg cccgccgcga ccggtgatcg tgatctcgac tcggtcggca
     6061 gcggccatca tcggcccggg attcaggccg atgtgcccgg gcggcaaccc aggccagttg
     6121 tgctggccgt acacctcgtc ggccggaaag cgcacgaaca gaccgtcgtc gatcatcgcc
     6181 ctggcgccgg catagccttc ctcgccgggc tggaatatca catacacggt gccgtcaaaa
     6241 ttgcgcgtgc cggcaaggta ctgcgcagcg ccgagcagca tcgtcgtatg gccgtcgtgg
     6301 ccgcacgcgt gcatgcagcc ctgtttcttg gagcgatggg ggaaatcgtt ctcctcgcga
     6361 atcggcagcg cgtccatgtc ggcgcgcagg ccgaccgact ttcccgacgc ttccgacttt
     6421 ccgcgtatca cgccgaccac gccggtgcgg ccgataccgg tgtgcacttc gatgccccac
     6481 tggtgcagcc gctcggcgac caggccggag gtgcgatgct cctcgaagcc gatctcggga
     6541 tgcgcgtgca ggtcgcgccg gatcgccgtc agcgcatcgt ggtgctgggc cacgaaactg
     6601 accggagcct tcagtgccgc gccgagatac gagcccatca gtgagccaac gcggtgcggt
     6661 cgatccgcag ccgggggtcg actgcgaagt acaacaggtc gacgatcagg ttgatcagca
     6721 cgaacacaag tgcgatcagg cacaggtacg cggccatcac tgggatgtcg gcgaactgaa
     6781 ccgactgaat gaacagcagc cccatgcccg gccactggaa cacggtttcg gtgacgatgg
     6841 caaatgcaat gatgccgccc agctgcagcc cggtgatcgt gatcaccggc accagcgtgt
     6901 tcttcagcgc atgaccgaag tgcaccgcgc ggttggtcaa gccgcgcgca cgcgcgaact
     6961 tgatgtagtc ggtgcgcagc acttcgagca tctcgctgcg caccagccgc atgatcagcg
     7021 tcagctgaaa caacgccagc gtgatcgacg gcaggatcag gtgcgtccac cccttcttgg
     7081 tcagcagtcc ggtggtccac cagccgagcg ccacagtctc gccgcgaccg taggacggca
     7141 gaacgcccag ttgcactgcg aagaccagga tcaaaaggat gccgatcagg aacgtcggca
     7201 gtgacacacc gagcagcgaa atcgcgagca acagctgtga cagccatgaa ttgcgccgca
     7261 gtgcggtgta cacccccatg gggatgccca cgaagatcgc gatcagggct gcggcaagtg
     7321 cgagttcgag cgtggcaggc agccgctcct ggatcagcgt cgacaccttg cggccctgcc
     7381 gcagactgat gccaaagtcc ccctgtactg cgtttgccac gaagtgaacg aactggacaa
     7441 agaacggctg gtccaaaccg aggtcggcgc gcagtcgggc gcgatcctcg ggcgtggcat
     7501 cctggccgag catctgtgcg accgggtcgc cgacatactg gaacaagagg aacgcgacga
     7561 atgccaccgc cagcatcacg agcacggctt ggagcagtcg gcgcatcacg aacgcagcca
     7621 tggagccttc tttgaaagcg cccggcccgc acacgttccg tgcggccagg ccgtcatcgg
     7681 cgcgaaatat acccttgcgg cccgacctgt cgtgttggtc cggactgaaa gaattggcgc
     7741 acgattgcga ctatccgaag tcagctgaaa ccgcctcgcg aggagcccca tgatgacccg
     7801 acgtaccttt ctgtccggcc tgaccggcct cgtggccggc gccgatgcca gcgccgcggc
     7861 aaagccgagt cccaaccgtc ccgaccgcct cgagctgccc gagtccgagt ggaagaagcg
     7921 cttgacgccc gagcagttcg acgttctgcg gcgcgagggt acggagcgcg ccttcacgag
     7981 ccccctcaac actgagaaac gcaagggcac ctatcactgc gtcgcgtgcg acctgccgct
     8041 attcgcgtcc gacatgaagt acgacagcgg caccggatgg ccgagcttct acacgaccct
     8101 gcccgacgcc ttcgagacaa gcaccgactt caagctgctg tatccgcgca ccgaatacca
     8161 ttgcgcgcgc tgcggcggtc atcacggcca cgtgttcaac gacggtccgg cgccaaccgg
     8221 caagcgttgg tgcaacaacg gcgtggcgct gaagttcgta ccggtgtgag ggcaccacga
     8281 tgatccgacg gctgattctc gcgctcgcgc tgtcgttggc ggcgggcgtc tcgttcgcgc
     8341 aggtgccggc cgcaccggcg accgcaaagg cgatctttgc cggcggctgc ttctggtgcg
     8401 tggagtccga cttcgacaag atcgacggcg tgatctcgac cacgtcgggc tataccggtg
     8461 gaaacgtgcc gaacccgacc tacgaacagg tgtcgagcaa gcgcaccggg cacgcggagg
     8521 cagtcgaggt cgtgttcgat ccgcgcaagg tcagctacgc acaactcgtc gagtacttct
     8581 ggcacacgat cgacccgacc acgaagaacc agcagttctg cgatgtcggc acgccgtacc
     8641 gcaccgcgat cttcgcgctc gacgccgagc agttgcgcat cgcgcaggac tcgaaggcgc
     8701 ggttggagaa gacaaagccc ttcaatgatc cggtcgtgac cgagatcgtg atggccggtc
     8761 cgttctaccc ggctgaggaa taccaccagg actactacaa gaagaacccg ctgcggtacg
     8821 cgtattaccg gaccgggtgc gggcgcgatg cgcggctgaa gcagttgtgg ggcgacaagg
     8881 ccgggcacat gtaatcgctt tcacggccag gtcggcaaca cacagcgctg gctgcgcagt
     8941 gccagcacgg catcgaagtc ggcgagtgcg cgccggacta ccttcgaatt ctccgcatga
     9001 cacgtcagac atgcgccaac acgcaggatg cgcttctgct cgtcgacgct gaaggggcgc
     9061 acgtcgtcgc gtgtcgacgt catatcgctg cgcgcctgca ggaagccgat ccatgcgtcg
     9121 gcgggcaggc cgtcgtacgg tgacggcggc cttttgggct cgaaagtcca gcggccgaca
     9181 ttgcccttga tttcatagcg cagcgagccg cttccgtaac cgagtgcgat cggatcgttg
     9241 tggcaggagc ggcatgaccg cgcctcgcgc cggatcgtgt gcataaaggt caggctatcc
     9301 aagcgacgaa agatcacgtc cggcggcttg ccagggtcgc ggttgcgatc gaaggtgagg
     9361 atcatgcctg ggacgaacgt ttcgatgcgg ccggcggcgc cacgctcgcc atggacgccg
     9421 agcgtgggcg gtgcgacctc gaacgggccg gcggtttcgt tccacgtgcc gcgcacccat
     9481 gcgcgttcga cgttgtcgta gccaacgtcg tcgggatcga acgcggtgtg acaggtcgtg
     9541 cagcgtggcg cccatgccga atggcagctc gcgcacgata gccgtccatg tcctgcgtca
     9601 cccgtgcacg ccggcagcgg cggcttcagg ggcaggagtt cgccggtccg cttgcggcgc
     9661 agctggcccc gtccatcacg gtccacgacc aggttgacca gcggctcgcc gttggcggcg
     9721 gcgcccatgc gctgcgacga agtcagggtc cagttccgca acgtgagcag cgtacgcgac
     9781 tcgggatcga tcgatgccag atcgatcgac gccagcctgt tcgcgtggca atccgtgcaa
     9841 cttatacgca gctgctcgct ctttcgcgcg acaacggcac cgctgcccat cacttcgttc
     9901 gacgtgtggc agtcgatgca gtccaggcca cgctcgtgat gcacgtccgg cacgacacgc
     9961 tcgagcgtgc ggccgtcgtc gagcggccgc agccgtgccg gatcggttgc gtcacgcggc
    10021 gcctggttca tctcgtgcca gccttcgtaa ctgagcgata tgcggctcga ccggctatgg
    10081 cagccgaagc agtgttcgtt ggtcggattg accgtcagcc ccgggtgtgt gcgcggaatc
    10141 gcattgcgct tctccggctc gattgcgaga tagcgcgcga gctccgcggc cgcgtccttt
    10201 ccatagacga gatggcatgc gttgcagccc ccgccgcgcg actcctggcc gatggcgccc
    10261 cattccgttt tcggttgacc gagatggcag gagacgcaca gctcgcgcaa gtggctgtcc
    10321 gcgacgccgt gtgccagacg atccgcgcgc ggcggcagcg cggtcggatc gaccggatct
    10381 ccaagcacgc gccgatcggt cgcgaccacg ccggccatcg tcgccatgat cgagcgctcg
    10441 acgcgcggcg caatcgcgac gtggcagcct gattgcccgc aggtgcggcg cgcatccgcc
    10501 agattgccgg gcacgcgaaa catgtcccga tgcgcgcgcc cggcatccag cgtgaacgga
    10561 tcgccgccat ggcaggccgc gcagccgagc acttcggggc gatgcgatcg atcgaagccg
    10621 ccgatgtccc gatggcatgc gcggcagccc tccgcgcggc cgcgcaccat cgcagcggcc
    10681 acggcgcccg cctgcggcgg cgtgccgcgc aggatccaac cgacttgcag atcgccgcgg
    10741 ctgtgaggcc acgccgagta acgaccgcct tcctcgcctc gaacgaagag tcccgacgca
    10801 cacaatccga gaaatcccag tgcgagcaga gccatggccg agcgcgctcg agcggtccac
    10861 ggcgagggca ggcgcggcag cagccacagc gcgagcaacg cggccagcgt gctggcaagc
    10921 gtcgcgaccg gcgcttgctg cagcaggtcc tgcagcgcga cgaagtacca cggtccccga
    10981 tagacaggat cgaggccgtc gtgcagtccg ggagagatga agagggaagt gagcgcaagc
    11041 gccgccgcca gcgccaccgc gatgcgcgcg cgcggccgcg acccgcgcac gaactcgagt
    11101 gcaacgacga cgagcagcac agacgcggca atgtgcagta cgtacgcggt gggccgccat
    11161 ttggcggcgg cgcgcagcgc cgacgcgaac ggcaacgcgt cgatagcccc ggtcaagacg
    11221 cgctggacct cgtgcgcatc gccgtcgccg cgcaggagga aaccgga
//
LOCUS       DBNHBY010000342         3301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1508950, whole genome shotgun sequence.
ACCESSION   DBNHBY010000342 DBNHBY010000000
VERSION     DBNHBY010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1508950"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16HB_08425"
     CDS             complement(<1..706)
                     /locus_tag="AC16HB_08425"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606857.1"
                     /translation="MSARRPVVRATVVVISGAGGGLGRALAHCYARAGARVVLLDRDA
                     AAVQRVAEELRSIARAPEPRALALPCDVTDESRCSAVIDATREAFGRIDIVINNAGIT
                     HRSAFERTDTAVLRRVMEVNLFGAIHLTRAALPALKATRGLVVAISSVAGFAPLIART
                     GYAASKHAVHGFFESLRSEVAAEGIDVMLVCPSFIATGIDRAALGPDGGAATHAQVVV
                     GQRLQPDEVADRIMHAG"
     gene            complement(703..1683)
                     /locus_tag="AC16HB_08430"
     CDS             complement(703..1683)
                     /locus_tag="AC16HB_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5606858.1"
                     /translation="MSGQRIFVTGGAGYLGSALLVTLAERLRRGEIDCVVASDVREVP
                     AAARQPGVVYERYDVRETGALEPLLRRHAIEVVVHLAAIVTPGAKSNRELEYAVDVTG
                     TRHVLEACVATGVRQIVVTSSGAAYGYHADNPEWLTETDPVRGNEAFAYSHHKRLVEE
                     MLADYRRDHPALRQVVLRVGTILGETTRNQITALFDKPRLLAIRGSRSPFVFIWDRDV
                     VGAIEHAIRTDASGVYNVAGDGALSIQEIAERLGKRCVTLPAGLLRAVLGVLHPLGLS
                     RYGPEQVDFLRYRPVLDNRRLKQELGYVPMKTSAEVFDFYCRAHGLGRQS"
     gene            complement(1680..2606)
                     /locus_tag="AC16HB_08435"
     CDS             complement(1680..2606)
                     /locus_tag="AC16HB_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR5606859.1"
                     /translation="MNELDAVRLNFSPESLIALDLILAFVLFGVALDMRPQDFKGILT
                     APRGTLIGLLAHSVLLPAVAWALTMVLQPAPSIALGMILVASCPSGNISNFLVNYGRG
                     NTALSVTIAAFSMLGSIFFTPFNLSFWGELNPATRPILHAVTLSPWEVFGTIVVLLGI
                     PTVLGMWVAYRWPQAAARMRKPFRLLSLVFFGLFIVGALAANWDFFLQYTGRVMLFVF
                     LLNAFALSTGYFTARAARLAEADRRAVALEVGIQNSGFGLALVFNFFGGLGGMAIVAA
                     WWGIWHLIVGLSLASIWRRFPPALPQPAQVTT"
     gene            complement(2603..>3301)
                     /locus_tag="AC16HB_08440"
     CDS             complement(2603..>3301)
                     /locus_tag="AC16HB_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5606860.1"
                     /translation="ARYRATIAAERAKLDEKLADLDVPMNPAHVAHVCQQVFPEGTTL
                     VADGGNTAIWTMFFHEMRVPNTLLSTFKFGMLGAGVGQALGAAVARPDHPVCCIIGDG
                     AMGFHPQEIETAVRNGLRVVYVVVCDRQWGMVKMNQQFMLKPLKTLIKKSLGPDETIK
                     ADLGEIRFDKVGEAMGAHGERVSDPRELRAALERALASGGPAVVHVDVDSVKHMWAPG
                     LMHFKDMHLEPKGK"
BASE COUNT          540 a         1123 c         1155 g          483 t
ORIGIN      
        1 cgccggcgtg catgatgcga tccgcgacct cgtccggctg cagccgctgg cccaccacga
       61 cctgcgcgtg ggtggctgcc ccgccatccg gtccgagcgc ggcccggtcg atgccggtgg
      121 cgatgaagga ggggcagacg agcatcacgt cgatgccctc cgcggccact tccgagcgca
      181 ggctctcgaa aaagccgtgc acggcgtgtt tgctggcggc atagcccgtg cgggcgatca
      241 gcggcgcaaa gcccgcgacg ctcgatatcg cgaccaccag tccgcgcgtg gccttcagcg
      301 ccggcaacgc agcccgcgtc aggtgaatcg cgccaaacag gttgacttcc atcacgcgtc
      361 gcagcactgc ggtgtcggtg cgctcgaacg cgctgcggtg agtgatcccg gcgttgttga
      421 tgacgatgtc gatgcgaccg aacgcctcgc gcgtcgcgtc gatgaccgcg ctgcagcgcg
      481 actcgtcggt gacgtcgcac ggcaaggcaa gcgcgcgcgg ttccggtgcg cgcgcaatgg
      541 accgaagttc ttccgccacg cgctgcacgg ctgccgcatc gcgatcgagc agcacgacgc
      601 gagcgcctgc ccgggcgtag cagtgggcga gggcgcggcc gaggccgcca cccgctccgc
      661 tgatcactac gaccgtggcg cggacgaccg gacggcgcgc gctcatgact gccgtccgag
      721 gccgtgtgcg cggcaataga aatcgaacac ctcggcggac gtcttcatcg gcacatagcc
      781 gagttcctgc ttgagccgac ggttgtccag caccgggcgg tagcgcagga agtcgacctg
      841 ctcgggaccg tagcgcgaca gcccgagggg gtgcagcacg ccgagcacgg cgcgcaacag
      901 gcctgccggc agcgtgacgc agcgcttgcc gagccgctcg gcgatctcct ggatgctgag
      961 cgcgccgtcg ccggccacgt tgtagacgcc gctggcgtcc gtgcggatgg cgtgctcgat
     1021 cgcgcccacg acgtcgcggt cccagatgaa gacgaacggg ctgcgcgagc cacggatcgc
     1081 cagcaggcgc ggcttgtcga acagcgcagt gatctggttg cgcgtggtct cgccgaggat
     1141 cgtgccgacg cgcagcacga cctggcgcag cgcggggtga tcgcgccggt agtcggccag
     1201 catctcttcg accagccgct tgtggtggga gtacgcgaag gcctcgttgc cgcggaccgg
     1261 gtccgtttcg gtcagccatt ccggattgtc ggcgtgatag ccgtaggcgg cgccgctcga
     1321 ggtgacgacg atctgccgca cgccggtcgc cacgcacgcc tcgagcacgt gccgcgtccc
     1381 ggtgacgtcg acggcgtact cgagttcccg gttggacttc gcccccggcg tcacgatggc
     1441 cgcgaggtgc acgacgacct cgatggcgtg acggcgcagc aggggctcga gcgcgccggt
     1501 ctcgcgtacg tcgtagcgct cgtacacgac gccgggctgg cgcgccgcag ccggcacctc
     1561 gcgcacgtcg ctggccacca cgcagtcgat ctcgccgcgc cgcaggcgct cggcgagcgt
     1621 cacgagcagc gcgctgccca gatagcccgc gccgcccgtg acgaagatcc gttgtccact
     1681 catgttgtca cctgcgcggg ctgcggcagg gccggcggga agcgtcgcca tatggaggcg
     1741 agcgagagcc cgacgatcag gtgccagatg ccccaccagg cggcgacaat cgccatgccg
     1801 ccgagcccgc cgaagaaatt gaagaccagt gcgagaccga acccggagtt ctggatgccg
     1861 acttcgagcg cgacggcgcg acgatcggct tcagccagtc gtgctgcgcg cgccgtgaag
     1921 taacccgtac tgagcgcgaa ggcgttgagc aggaacacga acagcatcac gcgacccgtg
     1981 tactgcagga agaagtccca gttggccgcc agcgcaccga cgatgaacag gccgaagaac
     2041 accagcgaga gcagccggaa cggcttgcgc atgcgcgccg cggcctgcgg ccagcggtat
     2101 gccacccaca tgcccagcac ggtgggtatg ccgagcagca cgacgatcgt gccgaacacc
     2161 tcccacgggc tcagcgtgac ggcgtgcagg atgggccgcg tcgccgggtt cagctcgccc
     2221 cagaacgaca ggttgaacgg ggtgaagaag atcgaaccga gcatcgagaa cgcggcgatg
     2281 gtcaccgaca gcgcggtgtt gccccggccg tagttcacca ggaagttgga gatgttgccg
     2341 ctcgggcagg acgcgacgag gatcatgccg agcgcgatgc tgggcgccgg ctgcagcacc
     2401 atcgtgagcg cccaggcgac ggctggcagc agcaccgagt gcgccagcag gccgatcagc
     2461 gtcccgcgcg gcgcagtcag gatgcccttg aagtcctgcg ggcgcatgtc gagcgccacg
     2521 ccgaacagca cgaaggcgag gatgaggtcg agcgcgatca gcgattccgg gctgaagttc
     2581 agccggaccg cgtcgagttc gttcatttcc ccttgggctc gaggtgcatg tccttgaagt
     2641 gcatcaggcc cggcgcccac atgtgcttga ccgagtcgac gtcgacgtgg accacggccg
     2701 gcccgccgct cgccagcgcc cgttcgagtg cggcgcgcag ttcacgcgga tcggagacac
     2761 gctcgccgtg cgcgcccatc gcctcgccga ccttgtcgaa gcggatctcg ccgaggtcgg
     2821 ccttgatggt ctcgtccggg cccagcgact tcttgatcag ggtcttgagc ggcttgagca
     2881 tgaactgctg gttcatcttc accatgcccc attgccggtc gcagaccacg acgtagacga
     2941 cgcgcaggcc gttgcgcacc gcggtctcga tctcctgcgg gtgaaagccc atcgcgccgt
     3001 cgccgatgat gcagcagacc ggatggtcgg gccgcgccac ggccgcgccg agcgcctgtc
     3061 ccacgcctgc gcctagcatg ccgaacttga acgtggagag cagcgtgttc ggcacgcgca
     3121 tctcgtggaa gaacatggtc cagatcgcgg tgttgccgcc gtcggcgacg agcgtcgtgc
     3181 cttccgggaa cacctgctgg cagacgtgcg cgacgtgcgc cgggttcatc ggcacgtcga
     3241 ggtccgcaag cttctcgtcg agcttcgcgc gctcggcggc gatggtcgcg cggtacctgg
     3301 c
//
LOCUS       DBNHBY010000343         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1712066, whole genome shotgun sequence.
ACCESSION   DBNHBY010000343 DBNHBY010000000
VERSION     DBNHBY010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1712066"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..990)
                     /locus_tag="AC16HB_08445"
     CDS             complement(70..990)
                     /locus_tag="AC16HB_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR5606861.1"
                     /translation="MNSMQAARAVHIGAALKYSALSAALLLALAGCGGTMPTMGGSKG
                     TVTGAAGGETAQGQNTQLTRCDETLGTLGVEEDSAAPWYVQLRQYNLGSTVPVLRLMI
                     QQSNCFVIVERSAAGMQMMQRERSLEQSGEMRGGSNFGKGQMVAADYTMRPSIQFAQK
                     GTGGIGGALGGLGFLGAAAGVVAGGLKQNEAATTLLLIDNRSGVQISAAEGSAKNYDF
                     NIAGGLFGGGLGAGAGAYSNTPQGKIITAAFADSYNQMVAALKSYKAQTVRGGLGTGG
                     RLGVDGGSTPASKELNQAPATSGAKPPAKK"
     gene            complement(1097..2203)
                     /locus_tag="AC16HB_08450"
     CDS             complement(1097..2203)
                     /locus_tag="AC16HB_08450"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HIR5606862.1"
                     /translation="MSLDDERFARMLHLASKTTPMNQAFAPPIVNTTVYALPGDPSGA
                     YQYARWGNPTWTALEAALSALEEAETITFPSGMAAIAAIFASVLKPGDRVLLPSDGYY
                     TTRICADKYLAPIGVAVETVATARLGAHKLAGYKLVLIETPSNPALDLADIRFNVERA
                     HAVGALVAVDNTTMTPLGQRPLDLGADFVVSADTKALNGHSDVLLGHVSSRSDELIAG
                     VRDWRRIVGAIPGPFEAWLVHRGLETLEVRFSRMCANALTLAQRLSAHRKVVEVRYPG
                     LPSHPAHALAQTQMASFGALIGVTLADKGAAESFIDTCRFIRPATSFGGVHTSAERRA
                     RWGDQVAEGFIRLSVGCEPTEVLWAEIDRALNEL"
     gene            complement(2207..>2985)
                     /locus_tag="AC16HB_08455"
     CDS             complement(2207..>2985)
                     /locus_tag="AC16HB_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606863.1"
                     /translation="DFATATPQDLAGVFQSRRATQRGAHSAVGRPLWPSKTDKRMVPD
                     GASLVGEHAFNGQGVPDGFRFSIGAAQDIMPTIELSQSEGATATLLEWKRVPHARAYF
                     IAAMGSRGSGSSPEIVFWSSSEVPDLGQGLLDYQTNAAVDRWLNEKVLLAPATTKCAI
                     PKGILGEAGMLRMIAYGTEMNVVHPPRPSDQKIAWEPQWAAKVRVKSVYQGLVGMDMP
                     AMPRGGGGSPEAAPPDAEPTKKDETKKPSALDILRGVLGR"
BASE COUNT          420 a         1079 c          988 g          498 t
ORIGIN      
        1 gcatcgccgg ttcgctgttt gtgggggacc ggcgattccg cggcagacac gcgagaaacc
       61 cgctcctcgc tacttcttgg ccggcggttt cgcccctgac gtggccggcg cctggttcag
      121 ctctttcgac gccggcgtgg agccgccgtc gacgccgagg cggccgccgg tgccgagccc
      181 gccgcgcacg gtctgcgcct tgtagctctt cagcgccgcg accatctggt tgtacgaatc
      241 ggcgaacgcc gcggtgatga tcttgccctg cggcgtgttc gagtacgcgc ctgcacccgc
      301 tccgagaccg ccgccgaaca acccgccggc gatgttgaag tcgtagttct tggcgctgcc
      361 ctcggccgcc gagatctgca cgcccgaccg attgtcgatc agcagcagcg tggtggccgc
      421 ctcgttctgc ttcagcccgc ccgcgaccac gccggccgcg gctcctagaa agcccagccc
      481 gccgagcgca ccgccgatgc cgccggtgcc cttctgcgcg aactggatgc tcggccgcat
      541 cgtgtaatcg gccgcgacca tctggccctt gccgaagttg ctgccgccgc gcatctcgcc
      601 cgactgctcg agcgagcgct ccctttgcat catctgcatg cccgccgcgc tgcgctcgac
      661 gatcacgaag cagttggact gctggatcat cagccgcagc accggcacgg tcgagccgag
      721 gttgtactgg cgcagctgga cgtaccacgg cgcggcgctg tcttcctcga cgccgagcgt
      781 gccgagcgtt tcgtcgcagc gcgtgagctg cgtgttctgg ccttgtgccg tttcgccgcc
      841 ggccgccccg gtgacggtgc ccttcgaccc acccatcgtg ggcatcgtcc cgccgcagcc
      901 ggcgagcgcc agcagaagtg cagccgacag cgcggaatac ttgagagctg cgccaatgtg
      961 cacggcgcgc gccgcttgca tcgagttcat tgccaccccc gctctggttg accgccgatc
     1021 ccccacggac tcggcgtggc gggactgtgc gaccgggggt gttactgcag cgttaccgcg
     1081 agcgacgagc cgcggttcag agctcgttca gcgcgcggtc gatctcggcc cagagcacct
     1141 cggtcggctc gcagccgacc gacaggcgga tgaatccctc ggcgacctga tcgccccagc
     1201 gcgcgcggcg ctcggcgctc gtgtgcacgc cgccgaagct cgtcgcaggg cgaatgaagc
     1261 ggcaggtatc gatgaagctt tcggccgcgc ctttgtcggc aagcgtgacg ccgatcagcg
     1321 cgccgaagct cgccatctgc gtttgcgcca gcgcgtgcgc cggatgtgac ggcaggcccg
     1381 gatagcgcac ctcgaccacc ttgcgatgcg cggacaatcg ttgcgccagt gtgagcgcat
     1441 tggcacacat gcgactgaag cgcacttcga gcgtctccag cccgcggtgc acgagccagg
     1501 cctcgaacgg tccggggatc gcaccgacga tgcgccgcca gtcgcgcacc ccggcgatca
     1561 gttcgtcgct ccgcgacgac acgtggccga gcagcacgtc cgagtgcccg ttcagcgcct
     1621 tcgtgtcggc gctgacgacg aagtcggcgc cgaggtcgag cgggcgctgg ccgagcgggg
     1681 tcatcgtcgt gttgtcgacc gcgacgagcg cgcccaccgc gtgcgcacgc tcgacgttga
     1741 accggatatc ggcgagatcc agcgcgggat tcgacggcgt ctcgatcagc acgagcttgt
     1801 agccggcgag cttgtgtgcg ccgagccgcg ctgtggcgac ggtctcgacc gccacaccga
     1861 tcggcgccag atacttgtcg gcgcagatgc gcgtcgtgta gtagccgtcg ctcggcagca
     1921 gcacgcggtc gccgggcttg agcacactgg cgaagatcgc cgcaatcgcg gccatgcccg
     1981 acgggaacgt gatcgtctcc gcctcctcca gcgccgacag cgccgcctcg agcgcggtcc
     2041 aggtcgggtt gccccagcgc gcgtactgat acgcgccact cgggtcgccc ggcagcgcgt
     2101 agacggtcgt gttgacgatc ggcggcgcga acgcctgatt catcggcgtc gtcttgctcg
     2161 ccaggtgcag catgcgcgcg aagcgttcgt cgtcgaggct catcgttcag cgtccgagaa
     2221 ctccacgcag gatgtcgagc gcgctcggct tcttcgtctc gtccttcttc gtcggctccg
     2281 cgtccggagg tgccgcttcc ggcgacccgc cgccaccccg cggcatcgcc ggcatgtcca
     2341 tgccgaccaa gccctgatac accgacttca cgcgcacctt ggccgcccac tgcggctccc
     2401 acgcgatctt ctgatcactc gggcgcggcg gatgcacgac gttcatttcg gtgccgtacg
     2461 cgatcatccg cagcatgccg gcctcgccga ggatgccttt cgggatcgcg cacttcgtcg
     2521 tcgccggcgc aagcaggacc ttctcattca accagcggtc gaccgccgcg ttggtctgat
     2581 agtcgagcaa cccctgcccc aggtcgggca cctcgctcga cgaccagaac acgatctcgg
     2641 ggctgctgcc gctgccgcgc gaccccatcg cggcgatgaa gtacgcgcgc gcatgcggca
     2701 cgcgcttcca ttcgagcagc gtggcagtcg cgccttcgga ctgcgacagt tcgatcgtgg
     2761 gcatgatgtc ctgcgcggcg ccgatcgaaa accggaaacc gtccggcacg ccctgcccgt
     2821 tgaacgcgtg ctcgccgacc agcgacgcgc cgtcgggcac catgcgcttg tcggtcttgc
     2881 tcggccacag tgggcggccg acggcgctgt gcgcgccgcg ctgggttgcg cgccgcgact
     2941 ggaacacgcc ggcgaggtcc tgcggcgtgg cggtcgcgaa gtcga
//
LOCUS       DBNHBY010000344         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1576474, whole genome shotgun sequence.
ACCESSION   DBNHBY010000344 DBNHBY010000000
VERSION     DBNHBY010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1576474"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="AC16HB_08460"
     CDS             <1..1668
                     /locus_tag="AC16HB_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5606864.1"
                     /translation="LRDVSLRVRHGVVTGLVGPDGAGKTTLLRLAAGLLTPAAGSISV
                     LGMDATRESLRVQSALGYMPQRFGLYEDLSVRENLDLYADLQGVPVRERPDRYAELMR
                     MTGLGPFSDRLAGRLSGGMKQKLGLACTLVRAPRLLLLDEPTVGVDPVSRRELWAIVY
                     RLVRDEGMSVLLSTAYLDEAERCDEVVLLHDGNVLAAGEPATLTTRMHGRAFAVTAPG
                     TSKRDVLARASALPGVIDGVIQADCVRVVTERAQTPDIDALLRDFPTAHIAPVPPRFE
                     DGFVALLRTTRAAPPAPPADGATMAAEARSGEVMIEVRDVKRRFGDFYAVNGVSFDVR
                     RGEVFGLLGPNGAGKSTTFRMLCGLLPASAGTLRVAGVDLRRAAAAARGRIGYMSQKF
                     SLYGNLSVRQNLDFFARVYGLSGRRHDERTEWALREFELGRHAESTSAELPLGYKQRL
                     ALACALMHEPDILFLDEPTSGVDPLARREFWGRINALAREQVTVLVTTHFMEEAEYCD
                     RLAIIAAGQLLAVGTPGEIKAAQQAETTMEDAFIRLIETFERRGHQP"
     gene            1722..>2117
                     /locus_tag="AC16HB_08465"
     CDS             1722..>2117
                     /locus_tag="AC16HB_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606865.1"
                     /translation="MRLFGLVRKEFLQVIRDPSSIAIAFIMPVALLLLFGYGVSLDSD
                     HVPVAVVIEDPGADAADFVAALDGSDYFAPIRVARMQTAIEALLDGRVDAIVHLRANF
                     AERLRRPDGAPIQVLINGVNANSANIVQGY"
BASE COUNT          326 a          719 c          728 g          344 t
ORIGIN      
        1 ctgcgcgacg tgagcttgcg cgttcgccac ggggtcgtga cgggtctcgt cgggcccgac
       61 ggcgcaggca agacgactct gctgcgcctc gcggcgggat tgctgactcc tgccgcggga
      121 agcatcagcg tgctcggcat ggatgcgacg cgcgagtcgc tgcgcgtgca gtccgcgctg
      181 ggctacatgc cgcagcgatt cggcctctat gaagacctga gcgtgcggga gaacctcgac
      241 ctgtatgccg atctgcaggg cgtgccggtt cgcgagcggc cggaccgcta cgcggaattg
      301 atgcgcatga ccgggctcgg accgttctcg gatcgcctcg cgggccgact ttccggagga
      361 atgaagcaga agctgggtct tgcctgcacg ctggtacgcg cgccgcgctt gctgctgttg
      421 gacgagccga ccgtcggcgt cgatccggtt tcgcggcggg aactgtgggc gatcgtctat
      481 cgcctggtgc gggacgaagg catgagcgtg ttgttgtcca ccgcgtatct ggacgaagcc
      541 gagcgctgcg acgaagtcgt actgctgcac gacggcaacg tgcttgccgc gggagagccc
      601 gcgacgctga ccacgcgcat gcacggccgc gcgttcgcgg tgacggcacc gggcactagc
      661 aagcgcgatg ttctggcgcg cgcatcggca ttgccgggcg tcatcgacgg cgtgatccaa
      721 gcggattgcg tgcgcgtcgt gaccgagcgg gcgcagaccc cggacatcga cgccctgctg
      781 cgcgacttcc cgacggcgca catcgcgccc gtgccgccgc gcttcgagga cggcttcgtc
      841 gccctgttac ggacgacgcg agccgcgccg ccggcaccac ccgccgacgg cgcaacgatg
      901 gcggcggaag cacgctccgg cgaagtgatg atcgaagtgc gtgacgtcaa acgccgcttc
      961 ggcgacttct acgcggtcaa cggagtttcg ttcgacgtgc gccgcggcga ggtgttcggg
     1021 ctgctcggtc ccaacggcgc cggcaagtcg acaacctttc gcatgctgtg cggcctgctg
     1081 ccggcatccg ccggcacgct gcgcgtcgcc ggcgtcgatc tgcggcgcgc cgcggcagcg
     1141 gcgcgcgggc gcatcggcta catgtcgcag aagttttcgc tctacggcaa cctcagcgtg
     1201 cggcagaacc tcgacttctt cgcccgtgtc tacgggttgt ccggacgccg ccacgacgaa
     1261 cggaccgaat gggcgctgcg cgaattcgag ctggggcgac acgccgaatc gaccagcgcc
     1321 gaactgccgc tcggctacaa gcagcggctg gcgttggcat gcgcgctgat gcacgagccg
     1381 gacatcctgt tcctggacga gcccacctcc ggcgtcgatc cgctggcgcg ccgcgaattc
     1441 tgggggcgga tcaacgcgct ggcgcgcgag caggtcaccg tgctggtgac gacgcatttc
     1501 atggaagagg ccgaatactg tgaccgtctc gcgatcatcg ccgccggcca gttgctggcg
     1561 gtcggcacac ccggcgagat caaggccgcg cagcaggccg agaccacgat ggaggacgcg
     1621 ttcatccgcc tgatcgaaac cttcgaacgt cgcggacacc aaccatgaaa gaaaccgcac
     1681 cctcgaagcc ctcctcgact ccccgccgcg ctggcgtcgc gatgaggctg ttcggcctgg
     1741 tgcgcaagga attcctgcag gtgatccgcg acccgagcag catcgccatc gcgttcatca
     1801 tgccggtcgc gctgctgctg ctgttcggct atggcgtatc gctcgattcc gatcatgtcc
     1861 cggtcgcggt cgtgatcgag gatcccggcg ccgacgcggc cgacttcgtc gccgcgctcg
     1921 acggctcgga ctactttgcg ccgatccgcg tggcacgcat gcaaaccgcc atcgaggcgc
     1981 tgctcgacgg ccgggtcgac gccatcgtgc acctgcgcgc gaacttcgcc gagcggctgc
     2041 ggcggcccga cggcgcgccg atccaggtgc tgatcaacgg cgtcaacgcc aactccgcga
     2101 acatcgtgca gggctac
//
LOCUS       DBNHBY010000345         7859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_629268, whole genome shotgun sequence.
ACCESSION   DBNHBY010000345 DBNHBY010000000
VERSION     DBNHBY010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_629268"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="AC16HB_08470"
     CDS             <1..79
                     /locus_tag="AC16HB_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5606866.1"
                     /translation="DIKDPTQNIVWVVPEGGGGPNYTNM"
     gene            complement(146..799)
                     /locus_tag="AC16HB_08475"
     CDS             complement(146..799)
                     /locus_tag="AC16HB_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR5606867.1"
                     /translation="MPRAYQPAVAVGVDAAGPPSGIRHSAFEDAQALVTGALFVALGF
                     ALFAHAGLLTGGTAGLAFLIHYLTGWRFGAVFFVLNLPFYWLAVRALGWQFTLKTFCA
                     VALLSLATETLPALLAFGTVSSLYAAVMGGFLVGAGLLMFFRHQASLGGVNVLVLYLQ
                     DRYGWRAGKAQMAIDGAIVLAAFAIVDLQRIALSVLGAIALNLVIAINHKPGRYLGV"
     gene            complement(772..1008)
                     /locus_tag="AC16HB_08480"
     CDS             complement(772..1008)
                     /locus_tag="AC16HB_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606868.1"
                     /translation="MKKVVAVIAASAIAGYALHALAQVRMDVRPAVVAIGSSSSNGIS
                     FAWFYDQTERTVFVCRAGHGSGDTVECRARTSLP"
     gene            1124..2407
                     /locus_tag="AC16HB_08485"
     CDS             1124..2407
                     /locus_tag="AC16HB_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR5606869.1"
                     /translation="MSYSNYHKKSILDRELHPETQMMSYGYDPFLSEGAVKPPVFLTS
                     TFAFRTAEDGADFFDVVAGRKPPPQDAGAGLVYSRFNHPNLEIVEDRLALLDGAQAAA
                     VTSSGMAAIGMVFLTLLRPGHQIVHSTPLYGGTETLIRKILPEWGVGGQPFVDGLSQT
                     EMEAALDAAAARGKVALFYIETPANPTNALVDFQAVAQALAAFERRHGYRPVTACDNT
                     MLGPIFQHPIRDGVDLSIYSLTKYVGGHSDLVAGGVTGRADLIQKLKLMRGAFGSQLD
                     PHSSWMIARSMETLVLRMRRAAATASRVAHWLADNPVRKVQVLHPQMIADPAYQAVYR
                     RQCTGAGSTFSIVLDGGRAPAFRFINALTLFKSAVSLGGTESLLCHPASTTHSGVPAE
                     VRAQVGVSEGLIRMSIGLEHEDDLIADLENALRQC"
     gene            2477..3025
                     /locus_tag="AC16HB_08490"
     CDS             2477..3025
                     /locus_tag="AC16HB_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606870.1"
                     /translation="MKCTLTVLTIVLVAVAAGCGKTKESATEKVAERMIESAMEKDGT
                     KAKVNLSETGAKITTTDAAGKTSQLELGTAQVTEADLGVPFYPGSKMMDGQSTRMSTP
                     EGTAVTVGLQSSDPPAKVAGFYRERLKAQAEGKQFTEMSSGDGAVLALADDKSRAAIQ
                     IAVNKAENGSEIAITTHRGTQK"
     gene            complement(3047..3550)
                     /locus_tag="AC16HB_08495"
     CDS             complement(3047..3550)
                     /locus_tag="AC16HB_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5606871.1"
                     /translation="MFLKVFEDRVRCPDGHVAPREYLRHPGAVMMIPLLDDGRLVLER
                     QYRYPLRKSFIEFPAGKIDAGESLLDCAQRELLEETGYRARDWSYLGSFHNAIGYCDE
                     QIYVFLARDLALDAARSDDGEVIEIFTATLSEVLDWIRAGTVTDVKTIIGAYWLQQHL
                     AGAWPAR"
     gene            complement(3690..5180)
                     /gene="nuoN"
                     /locus_tag="AC16HB_08500"
     CDS             complement(3690..5180)
                     /gene="nuoN"
                     /locus_tag="AC16HB_08500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR5606872.1"
                     /translation="MMENLNAWAAAPEIVLLAGALAVLAIDLFVSDARRNITYVLTLL
                     VLGATAAVCVALLADDTVAYSFGHMYVTDPFGHVLKLFATLCAALMLVYAQGYARDRG
                     LWKGELFTLSLFVLLGVFVMISGGNMLVIYLGLELQSLSLYALVALRRDDAKATEAAM
                     KYFVLGALASGFLLYGMSMLYGASGTLDIGQLARSIGAGEVPNRAALVLGTVFVVAGI
                     AFKLGAAPFHMWVPDVYHGAPTPVTLMIGSAPKIAAFAIAFRLLVEGLLPLAVDWQQM
                     LVLLALASLAIGNVTAIAQSNLKRMLAYSTIGQMGFMLLGMLSGVVGGDTLSTANAYS
                     AAMFYVITYALTTLGTFGIILLLAREGFEAEEIADLKGLNKRSPWFAFVMLLLMFSLA
                     GIPPTMGFYAKLVVLQALLGGAITTGIVWLAVIALGFSLIAAYYYLRVVKVMYFEEPS
                     DARPIVATQDMRVALSLNGALVLVLGILPQYLLALCANATIRALGG"
     gene            complement(5177..6670)
                     /locus_tag="AC16HB_08505"
     CDS             complement(5177..6670)
                     /locus_tag="AC16HB_08505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR5606873.1"
                     /translation="MLTYPWLSLSIWIPIFFGLVILAAGRDQNAGVVRTVALAGSLLG
                     LLVTLPLYLNFDAAQAGLQFVEKVLWIERLNAHYHLGVDGISIWFVLLTALMTVIVVI
                     AGWEVIEEKVAQYNAAFLILSGLMIGVFAAADGLLFYVFFEATLIPMYVIIGVWGGPN
                     RVYAAFKFFLFTLAGSLLMLIALIYLYYASGGSFDLATWNGLALPMNAQVLLFLAFFA
                     AFSVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFVRFSLPIVPDASHHLA
                     WFIIAISLVAVVYIGLVALVQTDMKKLVAYSSIAHMGFVTLGFFIFSQIGVEGALVQM
                     ISHGFISGAMFLCIGVMYDRMHSRNIADYGGVVNTMPKFASFMMLFAMANCGLPATSG
                     FVGEFLVILGAVKFNFWIGAVAATTMIWGAAYSLWMYKRVIFGDIANDHVRELTDIGG
                     REFLILALLAVAVLAMGVYPKPYTDLMHASVTELLQHVAKSKLPVGQ"
     gene            complement(6693..>7859)
                     /locus_tag="AC16HB_08510"
     CDS             complement(6693..>7859)
                     /locus_tag="AC16HB_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5606874.1"
                     /translation="VIGAITALFMGFLGIVQNDIKRVVAYSTLSQLGYMTVALGASAY
                     SVAIFHLMTHAFFKALLFLAAGSVIIGLHHDQDLRNMGGLRKYMPVTHWTSLIGTLAL
                     IGTPFFSGFYSKDNILIAAAAAHHAGQPGATFAYYAVMAGVFVTAFYSFRMYFLAFWG
                     KERFGGAHDHHGDHDDEEPSTDHHHGMAPGDKPHESPAVVWVPLVLLAIPSVMIGFVA
                     IDKFLFGGFFGTSIVVDAARHPAMNHLAEDFHGATAMALHGLTGLPFLLVIAAIGLAW
                     FLYLKRPDIPDAIAARFSMIYRLLDNKYYLDRINEFVFAGGARLIGRGLWKGGDVGVI
                     DNIAINGSARLVAWFAGVIRFAQSGYIYHYAFAMLVGIGVVLFFYLTLPYVMSR"
BASE COUNT         1553 a         2639 c         2547 g         1120 t
ORIGIN      
        1 cgacatcaag gacccgacgc agaacatcgt gtgggtcgtg cccgaaggcg gcggcggtcc
       61 gaactacacg aacatgtgat cggtccggtg ccgccgtgct cgacctcgcg gcggcgaacg
      121 gaattgcacg aacagcgagc ccgactcaaa cgccgagata ccgccccggc ttgtggttga
      181 tcgcgatcac gagattcagt gcgatcgcgc caagcaccga cagcgcgatg cgttgcaggt
      241 ccacgatcgc gaatgccgcc agcacgatcg cgccgtcgat cgccatctgc gccttgcccg
      301 cgcgccaacc gtagcggtcc tgcagataca gcaccagcac attgacgcca cccaggctcg
      361 cctggtggcg aaagaacatc agcaatcccg cgccgacgag gaagccgccc atcaccgcgg
      421 cgtaaaggga actgaccgtg ccgaacgcca gcagcgccgg caacgtctcg gttgcgagcg
      481 atagcagcgc aaccgcacag aacgtcttca gcgtgaactg ccagccgagc gctcgcaccg
      541 ccaaccagta gaaaggtagg ttgagcacga agaacaccgc gccgaagcgc cagccggtca
      601 ggtagtggat cagaaacgca agccccgccg tgccgcccgt caggagtccg gcatgcgcga
      661 acagtgcgaa gccgagtgcg acgaacaacg ctccggtgac gagcgcctgc gcgtcctcga
      721 acgcgctgtg gcggatcccg gacggggggc cggcagcatc gacgccgacg gctacggcag
      781 gctggtacgc gcgcggcact cgacggtgtc gccgctaccg tggccggcgc ggcatacgaa
      841 caccgtgcgt tcggtctgat cgtagaacca cgcgaacgaa atgccgttgg aagacgaact
      901 gccgatggcc accacggccg ggcggacgtc catacgcacc tgcgccagtg catgcaacgc
      961 atagccggcg atcgccgacg ccgcaatcac cgcaacaact ttcttcatcg tttcctcctc
     1021 gtacatgaac gggacatcat tgaacaacaa ttcgcgcccg cttgtcgtcg gccgtgaatc
     1081 ggccgacgat tcgcctgcac gacggaccaa ccggagacga accatgtcgt actcgaacta
     1141 ccacaagaag tcgatcctcg accgcgaact gcacccggaa acacagatga tgtcgtacgg
     1201 gtacgacccg ttcctgtccg agggcgccgt caagccaccc gtcttcctga cctcgacctt
     1261 cgcgttccgc accgcagaag acggcgccga tttcttcgac gtcgtcgccg gccgcaagcc
     1321 gccgccgcag gacgccggcg cgggactcgt gtacagccgc ttcaaccacc cgaacctgga
     1381 gatcgtcgag gaccggctgg cattgctcga tggcgcgcaa gccgccgcgg tcacttcgag
     1441 tggcatggcg gcgatcggca tggtgttcct gacgctgctg cggcccggcc accagatcgt
     1501 gcactcgacg ccgctgtatg gcggtaccga gacgctgatc cgcaagatcc tgcccgagtg
     1561 gggcgttggc ggacagccgt tcgtcgacgg cctgtcgcag acggagatgg aggccgcact
     1621 cgacgccgct gccgcgcgcg gcaaggttgc cctcttctac atcgagacgc cggcgaatcc
     1681 aaccaatgcg ctggtggact tccaggccgt cgcgcaggcg ctggccgcgt tcgagcgtcg
     1741 gcacggctac cggcccgtca cggcctgcga caacaccatg ctggggccga tcttccagca
     1801 cccgatccgc gacggcgtcg atctgtcgat ctactcgctg accaagtacg tcggcggcca
     1861 tagcgatctg gtcgcgggcg gcgtcaccgg ccgcgccgac ctgatccaga agctgaagct
     1921 gatgcgcggc gcgttcggct cgcagctcga cccgcattcg tcgtggatga tcgcgcgttc
     1981 gatggagacg ctggtgctgc gcatgcgccg cgccgccgcg accgcgagcc gggtggcgca
     2041 ctggttggcg gacaacccgg tgcgcaaggt gcaggtgctg catccacaga tgatcgcgga
     2101 ccccgcctat caagccgtgt accgccgcca atgcacgggg gccggctcga cgttctcgat
     2161 cgtcctcgac ggcggccgcg cgccggcgtt ccgcttcatc aacgcgctga cgctgttcaa
     2221 gtccgccgta agcctcggcg gaacggagtc gctgctctgc catccggcgt cgaccacgca
     2281 ctccggcgtg ccggccgagg tgcgggcgca ggtgggggtg tcggaaggcc tgatccgcat
     2341 gtcgatcggg ctcgaacacg aggacgacct gatcgccgat ctggaaaacg cgctgcggca
     2401 atgctgatac cgatcgacac ggtgcgcacc cggggatcag aattcagccc gtgccaaccg
     2461 agggaggggg tcgaccatga aatgcacgtt gacggtgttg acgatcgttc tggtggcggt
     2521 agcggcgggc tgcggcaaga ccaaggagtc cgccaccgag aaggttgccg agcggatgat
     2581 cgagtcggcg atggagaagg acggcaccaa ggccaaggtg aatctgtcgg agaccggcgc
     2641 caagatcacg acgaccgatg ccgcgggcaa gacgagtcaa ctcgaactgg gcacggcgca
     2701 ggttaccgag gcggatctgg gcgtcccgtt ctatccggga agcaagatga tggacggcca
     2761 gtcgacgcgc atgagcacgc ccgaaggaac cgccgtcacg gtcggcctgc agagcagcga
     2821 cccgccggcc aaggtggccg ggttctaccg ggaacgcctg aaggcgcagg ctgaaggcaa
     2881 gcagttcacc gagatgagca gcggtgacgg tgcggtgctc gcgctggcgg acgacaagtc
     2941 cagggccgcg atccagatcg ccgtgaacaa ggccgaaaac ggatccgaga tcgccatcac
     3001 cacgcatcgg ggcacgcaga agtagcgcac cgtgtggcgc cggcgctcag cgcgccggcc
     3061 atgccccagc cagatgctgc tgcagccagt acgcgccgat gatggtcttc acgtcggtga
     3121 cggtgccggc acgaatccag tcgagtacct cggagagcgt ggcggtgaag atctcgatca
     3181 cctcgccgtc gtcgctgcgc gccgcgtcga gcgccaggtc gcgcgcgaga aagacataga
     3241 tctgctcgtc gcagtagccg atcgcgttgt ggaaactgcc gagataggac cagtcgcgcg
     3301 cgcggtagcc ggtctcctcg agcagttcgc gctgcgcgca gtcgagcagc gactcgccgg
     3361 cgtcgatctt gcccgccggg aactcgatga agctcttgcg cagcggatag cggtactgcc
     3421 gctcgagcac cagccggccg tcgtcgagca gcggaatcat catcaccgcg cccggatgcc
     3481 gcaggtactc acgcggcgcg acgtgcccgt ccgggcagcg cacgcgatcc tcgaacacct
     3541 tcaggaacac cccctggtag gcgagcttcg actcaagcgt ttcttcggcg aggggatcag
     3601 gcgagacggg ctgcctcttt ttgccttgag tcctctccca cgcgcgggga gggtcgggag
     3661 gggcaaaccc ttgcgggcgt cgggcggcgc taaccgccca aagccctgat cgtcgcgttg
     3721 gcgcacagcg cgagcaggta ctgcggcagg atgccgagca ccagcaccag cgcgccgttc
     3781 agcgacaagg ccacgcgcat gtcctgcgtg gcgacgatcg ggcgcgcatc ggagggctcc
     3841 tcgaagtaca tcaccttgac cacgcgcagg tagtagtagg cggcaatcag cgagaagcca
     3901 agcgcgatca ccgccagcca cacgatgccg gtggtgatgg cgccgcccag cagcgcctgc
     3961 agcaccacca gcttggcgta gaaccccatc gtcggcggga tgccggcgag cgagaacatc
     4021 aagagcagca tcacgaacgc gaaccacggg ctgcgcttgt tcagaccctt caggtccgcg
     4081 atctcctcgg cctcgaagcc ttcgcgcgcc agcagcagga tgatgccgaa cgtaccgagc
     4141 gtggtcagtg catacgtgat cacgtagaac atcgccgcgc tgtacgcgtt ggcggtggac
     4201 agcgtgtcgc cgccgaccac gccggaaagc atgccgagca gcatgaagcc catctggccg
     4261 atggtcgaat acgcgagcat gcgcttcagg ttgctctgcg cgatcgccgt cacgttgccg
     4321 atcgccagcg atgccagcgc gagcagcacc agcatctgct gccagtcgac cgccagcggc
     4381 agcagcccct cgaccagcag ccggaacgcg atcgcgaacg cggcgatctt cggcgccgag
     4441 ccgatcatca gcgtcaccgg ggtcggcgca ccgtggtaga cgtccggcac ccacatgtgg
     4501 aacggcgcgg cgccgagctt gaacgcgatg ccggcgacga cgaacacggt accgagcacc
     4561 agcgccgcgc gattcgggac ctcgcccgcg ccgatgctgc gcgcgagctg gccgatgtcg
     4621 agcgtgccgc tggcgccgta cagcatcgac atgccgtaca gcaggaaccc cgaggcgagt
     4681 gcgccgagca cgaaatactt catcgccgcc tcggtcgcct tcgcgtcgtc gcgccgcaga
     4741 gcgaccagcg cgtacagcga cagcgactgc agctccagcc cgaggtagat caccagcatg
     4801 ttgccgcccg agatcatcac gaagacgccg agcagcacga acagcgacag cgtgaagagc
     4861 tcccccttcc acagcccgcg gtcgcgcgcg tagccctgcg cgtacaccag catgagcgcc
     4921 gcgcacagcg tggcgaagag cttcagcacg tgcccgaacg ggtcggtcac gtacatgtgc
     4981 ccgaaggaat aggcgaccgt atcgtccgcc agcagggcga cgcacaccgc cgccgtcgcg
     5041 ccgagcacga gcagcgtcaa cacgtaggtg atgttgcggc gtgcgtcact gacgaacagg
     5101 tcgatcgcca gcacggccag cgcgccggcg agcagcacga tctcgggcgc ggcggcccag
     5161 gcgttcaggt tctccatcat tggcccaccg gcagtttcga cttcgccacg tgctgcagca
     5221 gctccgtcac cgacgcgtgc atcaggtccg tgtacggctt cgggtacacg cccatcgcga
     5281 gcaccgccac cgcaagtagt gccaggatca ggaactcgcg cccgccgatg tcggtcagtt
     5341 cgcgcacatg gtcgttggcg atgtcgccga agatcacgcg cttgtacatc cacagcgagt
     5401 aggccgcgcc ccagatcatc gtcgtcgccg cgaccgcacc gatccagaag ttgaacttga
     5461 ccgcgcccag aatcaccagg aactcgccga cgaagccgct ggttgccggc aggccgcagt
     5521 tggccatcgc gaacagcatc atgaagctgg cgaacttcgg catcgtgttg accacgccgc
     5581 cgtaatcggc gatgttgcga ctgtgcatgc ggtcgtacat cacgccgatg cacaggaaca
     5641 tcgcgcccga gatgaagccg tgcgagatca tctgcaccag cgcgccttcg acgccgatct
     5701 ggctgaagat gaagaaaccc agcgtcacga agcccatgtg cgcgatcgac gagtaagcga
     5761 ccagcttctt catgtcggtc tgcaccagcg ccacgaggcc gatatacacc accgcgacca
     5821 gcgagatggc gatgatgaac catgccaggt ggtgcgacgc gtccggcacg atgggcagcg
     5881 agaaccgcac gaaaccgtac gccccgagct tcagcatgat cgccgccagc accaccgagc
     5941 cgccggtggg cgcctcgacg tgcgcgtcgg gcagccacgt gtgcaccggc cacatcggca
     6001 ccttgaccga gaacgcagcg aagaacgcca ggaacagcag cacctgcgcg ttcatcggca
     6061 gcgccagccc gttccacgtc gccaggtcga agctgccgcc cgacgcgtag tacaggtaga
     6121 tcagcgcgat cagcatcagc agcgagcccg ccagcgtgaa caggaagaac ttgaacgccg
     6181 catagacccg gttcggcccg ccccagacgc cgatgatgac gtacatcggg atcagcgtgg
     6241 cctcgaagaa gacatagaac agcagtccgt cggcggcggc gaacacaccg atcatcagcc
     6301 ccgacaggat caggaacgcg gcgttgtact gcgcgacctt ctcctcgatc acctcccagc
     6361 cggcgatgac gacgatcacc gtcatcagcg cggtcagcag cacgaaccag atcgagatgc
     6421 cgtccacgcc gaggtggtag tgcgcgttca gccgctcgat ccacagcacc ttctcgacga
     6481 actgcaggcc cgcctgcgcg gcatcgaagt tcaggtacag cggcaacgtg accagcaggc
     6541 cgaggagcga gccggcgagc gccacggtgc gcacgacgcc cgcgttctgg tcacggcccg
     6601 cagccaggat caccaggccg aagaagatcg gaatccagat cgacaggctg agccaagggt
     6661 aggtgagcat tattctttta tcccgccgcg gctcagcggc tcatcacgta cggcagggtg
     6721 aggtagaaga acagcaccac gccgatgccg accagcatcg cgaacgcgta gtgatagatg
     6781 tagcccgact gcgcgaaccg gatcacgccg gcgaaccagg cgaccagacg cgcgctgccg
     6841 ttgatcgcga tgttgtcgat cacgccgacg tcgccgccct tccacagacc gcgcccgatc
     6901 agccgcgcgc cgcccgcaaa cacgaactcg ttgatgcgat cgaggtagta cttgttgtcg
     6961 agcagcctgt agatcatcga gaagcgcgcc gcgatcgcgt ccgggatatc gggccgcttc
     7021 agatagagga accacgccag cccgatcgcc gcgatcacga gcaggaacgg caggccggtc
     7081 aggccgtgca gcgccatcgc cgtggcgccg tggaaatcct cggccaggtg gttcatcgcc
     7141 ggatgccggg ccgcatcgac gacgatcgag gtgccgaaga agccgccgaa caggaacttg
     7201 tcgatcgcga cgaaaccgat catcaccgac ggtatcgcca gcagcaccag cggcacccac
     7261 accacggcgg gcgattcgtg cggcttatcg ccgggcgcca tgccgtggtg atggtcggtg
     7321 ctcggctcct cgtcgtcgtg gtcgccgtga tggtcgtgcg cgccaccgaa gcgctccttg
     7381 ccccagaacg cgaggaaata catgcgaaac gaatagaacg cggtcacgaa cacgcccgcc
     7441 atcaccgcgt agtacgcgaa ggtcgcgccc ggctggccgg cgtggtgtgc ggccgcggcc
     7501 gcgatcagga tgttgtcctt cgagtaaaag cccgagaaga acggcgtgcc gatcagggcg
     7561 agggtgccga tcagcgaggt ccagtgcgtg accggcatgt acttgcgcag cccgcccatg
     7621 ttgcgcaggt cctggtcgtg gtgcaggccg atgatcacgc tgccggcggc gaggaacaga
     7681 agcgccttga agaaggcgtg cgtcatcagg tggaagatcg ccaccgagta ggcggaggcg
     7741 ccgagcgcca ccgtcatgta gcccagctgc gaaagcgtcg agtacgccac cacgcgcttg
     7801 atgtcgttct gcacgatgcc caggaagccc atgaagaggg cggtgatcgc gccgatcac
//
LOCUS       DBNHBY010000346         5513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_20303, whole genome shotgun sequence.
ACCESSION   DBNHBY010000346 DBNHBY010000000
VERSION     DBNHBY010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_20303"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="AC16HB_08515"
     CDS             complement(<1..198)
                     /locus_tag="AC16HB_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5606875.1"
                     /translation="MSAILTAERVNKSFGAVVAAADINVAIERDSVVGLIGANGAGKT
                     TFLNMITGYLQPTSGHIRFDGR"
     gene            complement(195..1133)
                     /locus_tag="AC16HB_08520"
     CDS             complement(195..1133)
                     /locus_tag="AC16HB_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606876.1"
                     /translation="MSTDRTVPILGVAATSLLAMAALAPQWLIFLLAVGLAKGLVVLG
                     LVLLMRTGLVSFGQGLYYGLGAYAAALLANFLGLTDAALMLLAGALASAGVAAVLGLL
                     LARYRQIFFSMLTLAFSMILYGLLVKSSALGSTDGFNLHARSFAGIALEGGDERRVFF
                     AVAVLLAFVAAWLLHRLLAGQWGRLSTAVADNELRVEYLGASAYRVVYVNYVASCALA
                     GVGGACTALAVGHVDPEMTYWTTSGEFVFIAILGGIGNVLAAFVSALLFEGLRTVANQ
                     YAPNVWQMTLGIAMLAIILFLPSGLWSLLRRMRRTA"
     gene            complement(1130..2026)
                     /locus_tag="AC16HB_08525"
     CDS             complement(1130..2026)
                     /locus_tag="AC16HB_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606877.1"
                     /translation="MTAIASVAFDGIVYSSWLFLMAAGLTLIYGVMRILNIAHGSFYA
                     LGAYTAASLVGAWISGGHAVYGSYPLLLVAAVLVGVIVGPPLERGLLRRMYGKDEVVL
                     LLVTYAVFLILEDAIKLVWGVESKVVSEPYAAPGNFEIGTLAYPVYNLVLIAGAIVTG
                     LLLAWALYRTRQGKLLLAVIHDREVSAAMGIDVGRVYLVTFTVGTALAALGGALTAPM
                     VAVSPGLSVEVIVLSFAVVVIGGLGSLGGAALGALLVGLVRGFAVRYWPEAELFTIYF
                     VMALVLIARPRGLFAVPEARKI"
     gene            complement(2126..3379)
                     /locus_tag="AC16HB_08530"
     CDS             complement(2126..3379)
                     /locus_tag="AC16HB_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606878.1"
                     /translation="MRAWLRYTLLALFALLALPAAAQQPVRIGVVTFLSGPAAGPFGV
                     PARNAAELTTEMLNAGAVPAPYATKGLGGNPIELVIIDEAGGPQKQVAEFRNIAQRVD
                     LVIGYISSGDCLAIAPVAEELKKLTVLFDCGTPRIFEDASYKYAFRTGGTATMDNVSA
                     AMYLLEKHPNVKSIAGINQNYAWGQDSWVDFEATMKALRPSIEGKTSQMTKLMAGQFG
                     AEISALLASGADVIHSSFWGGDLEGLVLQGAPRGLFQKHVVVLSAGEPAIFRLGTKIP
                     EGTIIGARGPFGPFAPDTALARWFRSAYQDRYQVVPNYASYKMATAILGVKAAYEKAG
                     GPKPTQEQVIAAFEGLTFEGPGGQVRMALGKGHQAVMDVAVGTVKHAGGELQVVDIKR
                     YPADKVNPPEGVKSEAWIKSGFKSR"
     gene            complement(3472..4164)
                     /locus_tag="AC16HB_08535"
     CDS             complement(3472..4164)
                     /locus_tag="AC16HB_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606879.1"
                     /translation="MTAVLRVRNLNAWYGPAQALFDLSFDLNAGEIVALIGRNGAGKS
                     TALKAIMGLVRATGTIELDGERIDARASHARARRGLGYVPEDRRIFTDLTARENLLVG
                     AQRRTLELEPLLVLFPNLRELLDRSAAQMSGGEQQMLAVARTLAARPRVVLLDEPSEG
                     IAPRIVAALAETLAQMKAQGVSVVLSEQNARFVARVADRALLLDRGRSVGAATIREVT
                     ELAPAVRGVLGL"
     gene            complement(4161..4904)
                     /locus_tag="AC16HB_08540"
     CDS             complement(4161..4904)
                     /locus_tag="AC16HB_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606880.1"
                     /translation="MTLLALDGVRKHFAGVHAVDGVTLGIAAGEFTALIGPNGAGKTT
                     LLSTIAGQLRADAGRISFEGRRIDGAAPARLSRLGIGRTFQTAHVFASMTVLENVLLA
                     LAVRAGAQFDFTGPLWHRDATVARRLLDRVGLGAYAEASASRLGYGDVKRLEFALALA
                     GAPRLLLMDEPTAGMAPGERLALMDLVRDIARETGLTVLFTEHSMEVVFGYAQRVIVM
                     SSGVVIADGAPEAVRADAAAQAAYFGAEP"
     gene            complement(4901..>5513)
                     /locus_tag="AC16HB_08545"
     CDS             complement(4901..>5513)
                     /locus_tag="AC16HB_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5606881.1"
                     /translation="RAQGVYAAMLTLAFAQILWSVAVQWIEVTGGDNGVLGLWPPDLL
                     APKSAYFLFTLLLALASLTLLRRAALAPFGFALRAARDAPDRAAASGIDTLRVQWAAF
                     AAGGLFAGLAGVLNAYQKGSVFPNVLAIPQSLDALVMVLLGGLQTLSGPIVGALSYHA
                     LTVELTRATEHWRLALGLAIVLLVLAFPQGIAGFVRARWEPES"
BASE COUNT          971 a         2020 c         1783 g          739 t
ORIGIN      
        1 gcgcccgtcg aagcgaatgt gtcccgaagt cggctgaaga tagccggtga tcatgttcag
       61 gaacgtcgtc ttgccggcgc cgttcgcgcc gatcaggccg accacgctgt cgcgctcgat
      121 cgcaacgttg atgtccgccg ccgcgaccac cgcgccgaac gacttgttga cccgctcggc
      181 cgtgaggatc gcgctcatgc ggtgcgccgc atccgccgca gcaatgacca cagtccgctc
      241 gggaggaaca ggatgatcgc cagcatcgca atgccgagcg tcatctgcca cacgttcggc
      301 gcgtactggt tggccaccgt gcgcagtccc tcgaacagca gcgcagagac gaacgccgcg
      361 agcacattgc cgatgccgcc gagaatcgcg atgaagacga actcgccgga cgtggtccag
      421 tacgtcattt ccggatcgac gtgtccgacc gcgagcgccg tgcacgcgcc gcccacgccc
      481 gcaagcgcgc acgaagcgac gtagttgacg tacacgaccc gatacgcgga ggcgccgagg
      541 tactcgacgc gcaactcgtt gtcggccacc gcggtcgaca accggcccca ctgtccggcg
      601 agcagccggt gcagcagcca cgccgcgacg aacgccagca gcacggcgac cgcgaagaac
      661 acgcgccgct catcgccgcc ttcgagcgcg atgccggcga aactccgcgc gtgcaggttg
      721 aagccatcgg tgctgccgag cgccgagctc ttgaccagca ggccatacag gatcatcgag
      781 aacgccagcg tcagcatcga gaagaagatc tgccggtagc gcgccagcag cagcccgagc
      841 accgccgcca cccccgcgct cgccagcgca ccggcgagca acatcagcgc ggcgtcggtc
      901 agtcccagga aattggccag aagtgcggcc gcgtacgcgc cgagtccgta gtacagcccc
      961 tgcccgaacg aaacgagccc ggtgcgcatc agcagcacca gcccgagcac gacgagcccc
     1021 ttggcaagac cgaccgcgag caggaatatc agccactgcg gcgctagcgc cgccatcgca
     1081 agcagcgagg ttgccgcgac gcccaggatc gggaccgtcc ggtcggtgct catatcttgc
     1141 gcgcctccgg caccgcgaac agcccccgag gacgtgcgat cagcaccagc gccatcacga
     1201 agtagatcgt gaacagttcc gcctccggcc agtagcgcac cgcgaaccca cgcaccagtc
     1261 ccaccagcag cgcgccgagc gccgcgccgc cgaggctgcc gagcccgccg atcacgacga
     1321 ccgcgaacga cagcacgatc acttccacgc tcagccccgg actgaccgcg accatcggcg
     1381 cggtcagcgc accgccgagc gcggcgagcg cggtgccgac cgtgaacgtc acgagataca
     1441 cgcgcccgac gtcgatgccc atcgccgcgc ttacctcgcg atcgtggatc accgccaaca
     1501 gcagcttgcc ctgccgcgtg cgatacagcg cccaggcgag cagaagtccc gtgacgatcg
     1561 caccggcgat cagcaccagg ttgtagaccg ggtaagcgag cgtgccgatc tcgaaattgc
     1621 cgggggccgc gtatggctcg gagacgacct tcgactccac gccccagacg agcttgatcg
     1681 cgtcctcgag gatcaggaac accgcatagg tcaccagaag cagcacgacc tcgtccttgc
     1741 cgtacatgcg cctgagcagg ccgcgctcga gcggcggccc aacgatcacg ccgaccagca
     1801 ccgcggccac gagcagcagc ggatagctgc cgtaaaccgc gtgtccaccg gaaatccagg
     1861 caccgaccag cgacgccgcg gtgtacgcgc cgagcgcata gaagctgccg tgcgcgatgt
     1921 tcaggatccg catcacgccg tagatcagcg tcagccccgc ggccatcagg aacagccagg
     1981 acgagtagac gatgccgtcg aaggcgaccg aggcgatcgc tgtcatggcg acatggcgag
     2041 cacgccgcaa acttcgagca gccgcacgcc tgcgcggccc gctcgaacat gcggggctag
     2101 gccggcgcgc atcgcgcgtc cggccctagc gggacttgaa gccggacttg atccacgcct
     2161 cgctcttcac gccctccggc ggattcactt tgtccgccgg atagcgcttg atgtcgacga
     2221 cctgcagttc gccgcccgcg tgcttgaccg tgccgactgc cacgtccatc accgcctggt
     2281 gccctttgcc gagggccatc cggacctgtc cccccgggcc ttcgaacgtc aggccctcga
     2341 acgcggcgat gacctgctcc tgcgtcggct tcggtccccc agccttctcg tacgcggcct
     2401 tcacgccgag gatcgcggtc gccatcttgt atgacgcata gttgggaacg acctggtagc
     2461 gatcctggta cgcgctcctg aaccagcgcg cgagcgccgt atccggcgcg aacggtccga
     2521 aggggccgcg cgcgccgatg atcgtgccct ccggaatctt ggtgccgagg cggaagatcg
     2581 ccggctcgcc cgccgacagc acgacaacgt gcttctggaa cagcccgcgc ggggcgccct
     2641 gcagcaccag cccttcgaga tcgccacccc agaagctgga gtggatcacg tcggcgccgc
     2701 tggcgagcag cgccgagatc tcggcgccga actggccggc catcagtttg gtcatctgcg
     2761 aggtcttgcc ctcgatcgac ggccgcagcg ctttcatcgt cgcctcgaag tcgacccacg
     2821 aatcctggcc ccacgcgtag ttctgattga tgccggcgat cgacttgacg ttcgggtgct
     2881 tctcgagcag gtacatcgcg gccgacacgt tgtccatcgt cgccgtgccg ccggtgcgaa
     2941 acgcgtactt gtaggaggcg tcctcgaaga tgcgcggcgt gccgcagtca aagagcaccg
     3001 tgagcttctt cagctcctcc gccaccggcg cgatcgccag gcaatcgccg ctcgagatgt
     3061 agccgatcac gaggtcgacg cgttgcgcga tgttgcggaa ctcggccacc tgcttctgcg
     3121 gcccgcccgc ctcgtcgatg atcacgagct cgatcgggtt gccgcccagg cccttggtcg
     3181 cgtacggcgc cggcaccgcg ccggcgttca gcatctccgt cgtcaattcg gcggcgttgc
     3241 gcgccggtac gccgaacggt ccggcagcgg ggccggacag gaaggtgacg acgccgatgc
     3301 gcaccggctg ctgtgcggca gccggaagcg ccagcagcgc aaacaaggca agcagtgtgt
     3361 agcgaagcca ggctctcatc gtggttctcc tccgaagggc ggtcgtgccg cgaatgcgcg
     3421 caaagcctac gcctgccgga aagtcgacgg aagccggccg acgcgcgcgg gctaaaggcc
     3481 gagcacgccg cgcaccgccg gcgcaagctc ggtcacctcg cggatcgttg cggcaccgac
     3541 gctgcggccg cgatcgagca gcagcgcgcg atcggccacg cgcgcgacga agcgcgcgtt
     3601 ctgctccgac agcacgacgc tgacgccctg cgccttcatc tgcgcgagcg tttcggccag
     3661 cgccgcgacg atcctgggcg cgatgccctc cgacggctcg tcgagcagca cgacgcgcgg
     3721 ccgcgcggcc agcgtgcgcg caaccgccag catctgctgt tcgccgccac tcatctgcgc
     3781 ggccgagcgg tcgagcagtt cgcgcaggtt cggaaacagc accagcagcg gctcgagttc
     3841 gagcgtgcgg cgctgcgcgc cgaccagcag gttctcgcgc gcagtcagat cggtgaagat
     3901 gcgccggtcc tcgggcacgt aacccagccc gcggcgggca cgcgcatggc tggcgcgcgc
     3961 gtcgatccgc tcgccgtcga gttcgatcgt tccggtcgcg cgcaccaggc ccatgatcgc
     4021 tttcagagcg gtcgacttgc cggcgccgtt gcggccgatc agcgcgacga tctcgcccgc
     4081 gttcaagtcg aagctcaggt cgaacagcgc ctgcgccggg ccataccagg cgttcaggtt
     4141 gcgcacgcgc agcacggccg tcatggctcc gcgccaaaat acgcggcctg cgcggccgcg
     4201 tctgcgcgca ccgcctccgg cgcgccgtcg gcgatcacca cgccgctcga catcacgatc
     4261 acgcgctgcg catagccgaa cacgacttcc atgctgtgct cggtgaacag cacggtgaga
     4321 cccgtttcgc gcgcgatgtc gcgcaccagg tccatcaacg cgaggcgctc acccggtgcc
     4381 atgccggcgg tgggctcgtc catcagcagc agccgcggcg cgccggccag cgcaagcgcg
     4441 aactccagcc gtttcacgtc gccgtagccc aggcgcgacg ccgacgcttc cgcataggcg
     4501 ccgagcccga cgcggtcgag cagccggcgc gcgaccgtcg catcgcggtg ccacagcggg
     4561 ccagtgaagt cgaactgcgc gccagcgcgc acggcaagcg caagcagcac gttctcgagc
     4621 accgtcatcg atgcgaacac gtgcgcggtc tggaacgtcc ggccgatgcc gaggcgcgac
     4681 aggcgtgccg gcgctgcacc gtcgatgcgg cgcccctcga agctgatccg gcccgcgtcg
     4741 gcgcgcaact gcccggcgat cgtcgacagc agcgtggtct tgccggcgcc attgggaccg
     4801 atcagtgcgg tgaattcgcc cgctgcaatg ccgagcgtca cgccgtcgac cgcgtgcacg
     4861 ccggcgaaat gcttgcgcac gccgtcgagt gccagcagcg tcatgattcc ggctcccagc
     4921 gcgcgcgcac gaagcccgca atgccctgcg gaaacgcgag caccagcagc acgatcgcca
     4981 gcccaagcgc gaggcgccag tgctcggtcg cacgcgtcaa ctcgaccgtc aaggcgtggt
     5041 acgacagcgc gccgacgatc ggaccggaca gcgtctgcag tccgccgagc agcaccatca
     5101 ccagcgcatc gagcgattgc gggatcgcca gcacgttagg aaacaccgaa cccttctggt
     5161 aggcgttcag cacccccgcc agcccggcga agagtccgcc ggcagcaaac gcggcccact
     5221 gcacgcgcag cgtgtcgatg ccgctggcgg cggcgcggtc gggcgcgtcg cgcgcggcgc
     5281 gcagcgcaaa gccgaacggc gcgagcgccg cgcgtcgcag cagcgtcagg ctcgccaatg
     5341 cgaggagcag cgtgaacagg aagtaggcgg atttcggcgc cagcaaatcc ggcggccaca
     5401 ggccgaggac accgttgtcg ccgcccgtca cctcgatcca ctgcaccgcc accgaccaca
     5461 ggatctgcgc gaacgcgagg gtcagcatcg ccgcgtacac gccctgcgcg cgc
//
LOCUS       DBNHBY010000347         6513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1441034, whole genome shotgun sequence.
ACCESSION   DBNHBY010000347 DBNHBY010000000
VERSION     DBNHBY010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1441034"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1949
                     /gene="dnaE"
                     /locus_tag="AC16HB_08550"
     CDS             <1..1949
                     /gene="dnaE"
                     /locus_tag="AC16HB_08550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5606882.1"
                     /translation="EGLTRNVGMHAGGVLIAPGKLTDFCPLYCAQGTDAVISQFDKDD
                     VEAIGLVKFDFLGLTTLTILDLTLRFVKQLDPASTITLEDLPLDDAKTYEVFRRADTT
                     AVFQFDSQGMRGMLKRARPSVLEDLIALNALYRPGPMDLIPEFCDRKHGKKIEYLDPR
                     LEPILGPTYGVMTYQEHVMTIAQVVGGYTLGGADLLRRAMGKKKPDEMAKQRAIFLKG
                     AVERGMPENKAQLLFDQMEKFAGYGFNKSHSAAYALVAYQTAYFKSHHPAAFMAANLT
                     ALAGDTDSVKVLVEDSRALGLAVEAPDVNRGAYRFEPIDAKTIRYGLGAIKGTGQGAI
                     EHILAVRESGYFRDLFDFCARVDRRIVNRRVVEALVRAGAFDATDADRARLLASVGRA
                     MEAADHAAASAGQASLFGGALESSTVEYVAAAPWSDRERLANEKLALGYYFSGHLFKE
                     YEAEARRLASLKLADVRPVAAGPGARSDPIRIAGIIVSARAQNTRRGRIGVIVLDDAT
                     AAIEITVFAELFDRRRALLKEDTLVFVTGRVRAGDGERVSVRADEIIDLAEARARAQA
                     ALRIDVDGVTSVAKLKDALVPYRISNGSGEGVAGCRVVLHVANRSGCADLRLPDAWRV
                     RGDERLLDELRAQPRVRGVECSYG"
     gene            complement(1940..2758)
                     /locus_tag="AC16HB_08555"
     CDS             complement(1940..2758)
                     /locus_tag="AC16HB_08555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5606883.1"
                     /translation="MLVSVIVATYNRPDALRAVLAGFCEQTDRGFELLVADDGSRDDT
                     RALVESIAPSAPIPIAHVWHEDTGFRLAAIRNRAAAQARGDYLVFVDGDCVPLADFVA
                     QHRSLAEPHWMVAGNRLLLSEGFTRRVLAEQVPIHRWTLSQWRAARRRGDINRVLPLL
                     RLPLGPLRRLGGSRWPRVRGCNMAVWRRDLARVNGFDESFQGWGYEDSDFAVRLVNAG
                     VGLKKGAFATGLLHLWHREHDRAREGANWERLQQRIRSRETRAELGLDRYTAQP"
     gene            complement(2759..3532)
                     /locus_tag="AC16HB_08560"
     CDS             complement(2759..3532)
                     /locus_tag="AC16HB_08560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5606884.1"
                     /translation="MDNAPRLSVAIITRNEAHNIADCLASVAFADEIVVVDSGSTDAT
                     VTLARQHGARVIEADWPGFGVQKNRAVDACHGEWILSIDADERVSADLRAQIEHALAA
                     PRFDVYEMPRRSYYVGRFMQHGGWWPDHCVRLFRRGTARFSDTRVHEGLVTDRPIGRL
                     SAPLVHYSFRTMEAVLDKINHYSSASAQTLAERGAAPGLATAILHGAAAFLRTYVFKL
                     GFLDGKHGFMLAVSNAEGSYYRYVKAMLLADRRQRDKTD"
     gene            complement(3532..4407)
                     /locus_tag="AC16HB_08565"
     CDS             complement(3532..4407)
                     /locus_tag="AC16HB_08565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5606885.1"
                     /translation="MAEPRASLPTVSVVTPTRDRPTLLPRALRSLLAQRHADWEWIVI
                     DDGSAAGATAAIDRADARIHLHRNAAASGAAAARNRGIALASAPLVAFLDDDDEYEPE
                     YLGAVVTAFARQPAASFCMPGVTRVYADGTERAEPFDADAADIDFLRKASASRGLALR
                     TATLRQLGGFDESLGVSEDIDLLMRAVAAGARALFLREPLVRIHEHAGTSLSRASKAS
                     VHVAASESLWRRHQPIFAAHRALWRHYGGVHAANLYRAGRRVEARAMLRELASHVDAV
                     PRALEILFRFECRPH"
     gene            4453..5736
                     /locus_tag="AC16HB_08570"
     CDS             4453..5736
                     /locus_tag="AC16HB_08570"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5606886.1"
                     /translation="MVSGVPRALTAGEATAAREARLLTAARACALLALIGAFVKPVAA
                     NWGAGLMLLAFALLPTAGTRLRALLREPLPRAALLLLAVMALSMLWSSAPWPERFAHF
                     WSWRTLLVLIVSLAVFDSRAWKLRLVLTIVAATLVGAVAAWITWALDYRVYPIHPAGS
                     VLRNGVTQGLAFAVAAFLAVVAALFEHGLSRRLRIALIGAAAVLVASLFFVTAGRSAQ
                     IGLVVMTAAAALLLLRGRARRVALFAIPVMFAAGVLLAPMAKERFVIGWQEATRIDGA
                     ADGGSVGFRLLLWRETARLIADRPLLGYGSGGFAAAYNERVERQYTDWRRRQGTDPHN
                     QYLWVQVRAGVLGTLAFAWFLLSAARQSAPQPYRSAALAILAMWCVTSLANSHFESFN
                     EGHMIALLLGCLLARESDRDLQAESPRITAFSTSS"
     gene            complement(5691..>6513)
                     /locus_tag="AC16HB_08575"
     CDS             complement(5691..>6513)
                     /locus_tag="AC16HB_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR5606887.1"
                     /translation="VCGWSHPLFAAYPWPVQALAAQRAERLRALRAWRVAHPGGRGLL
                     FTNSFSTALEFRLAGLRPLGYAADGRSLLLSRAVARPRIGRATHMVEYYYRLAAQLVP
                     HAPASPGELNLRVTNEARRRACELLRAAGVAGPYVVLCPVAVGRHYGKVKAWSGFGAL
                     ADALRARGVTVVACPGPGEREAVLQAIPNAALLAETDVGAFAALLAASRLVVANDSGS
                     GHLAAAVGVPLVSVFGVTDPTATRPWGARVILVGSATGWPAYDEVEKAVMRGLSA"
BASE COUNT          978 a         2251 c         2308 g          976 t
ORIGIN      
        1 tcgaggggct gactcgcaac gtcggcatgc acgcgggcgg cgtgctgatc gcgccgggca
       61 agctcaccga cttctgcccg ctgtactgcg cgcagggcac cgatgccgtc atcagccagt
      121 tcgacaagga cgacgtcgag gcgatcggcc tggtgaagtt cgacttcctc gggctgacga
      181 cgctgacgat cctcgacctc acgctgcgct tcgtgaagca gctcgacccg gcgtcgacga
      241 tcacgttgga agacctgccg ctcgacgacg cgaagaccta cgaggtgttc cgccgcgccg
      301 acacgaccgc cgtgttccag ttcgactcgc agggcatgcg cgggatgctc aagcgcgcgc
      361 ggccgagcgt gctcgaggac ctgatcgcgc tgaacgcgct gtaccgcccc gggccgatgg
      421 acctgattcc ggagttctgt gaccgcaagc acggcaagaa gatcgagtac ctcgatccgc
      481 ggcttgagcc gatcctcggc ccaacgtatg gcgtgatgac ctatcaggag cacgtgatga
      541 ccatcgcgca ggtcgtcggc ggctacacgt tgggcggcgc cgacctgctg cggcgggcga
      601 tgggcaagaa gaagcccgac gagatggcca agcagcgcgc gatcttcctc aagggcgcgg
      661 tcgaacgcgg catgcccgag aacaaggcgc agctgctgtt cgaccagatg gagaagttcg
      721 ccggctatgg cttcaacaag agccattccg ccgcctacgc gctggtcgcc taccagaccg
      781 cgtacttcaa gtcgcatcat ccggccgcgt tcatggccgc caacctgacc gcgctcgctg
      841 gcgacaccga cagcgtgaag gtgctggtcg aggacagccg cgcgctcggc ctcgcggtcg
      901 aggcgccgga cgtgaaccgc ggcgcgtacc gcttcgagcc gatcgatgcg aagacgatcc
      961 gctacgggct gggggcgatc aaaggcacgg gccagggtgc gatcgagcac atcctcgccg
     1021 tgcgtgaaag cggttacttc cgcgacctgt tcgacttctg cgcgcgcgtc gatcgccgca
     1081 tcgtcaaccg gcgcgtggtc gaggcactgg tgcgcgcggg cgcgttcgac gccaccgatg
     1141 ccgatcgcgc caggttgctc gcaagcgtcg gccgcgcgat ggaggcggcg gaccacgccg
     1201 ccgcgagtgc cgggcaggcg agcctgttcg gcggcgcgct ggagagttcg acggtcgagt
     1261 atgtggcggc ggcgccttgg agcgaccgcg agcggctggc gaacgagaag ctcgcgctcg
     1321 gctattactt cagcggccac ctgttcaagg agtacgaagc cgaagcgcgc cgccttgcat
     1381 cgctgaagct cgccgacgtg cggcccgtcg ccgcagggcc gggcgcgcgc agcgatccga
     1441 tccgcatcgc cggcatcatc gtgtcggcgc gcgcgcagaa cacgcggcgc ggccgcatcg
     1501 gcgtgatcgt gctcgacgat gccacggcgg cgatcgagat taccgtgttc gcggaactgt
     1561 tcgaccgcag gcgcgcgctg ctgaaggagg acacgctggt gttcgtcacg ggccgcgtgc
     1621 gcgccggcga cggcgaacgt gtctcggtgc gggctgacga aatcatcgat ctggccgagg
     1681 cgcgcgcgcg cgcgcaggcg gcgctgcgaa tcgacgtcga cggcgtgacg agtgtcgcga
     1741 agctgaagga cgcgctggtg ccgtatcgga tctcgaacgg cagcggcgaa ggcgttgccg
     1801 gctgccgtgt cgtcctgcac gttgccaacc gcagcggctg cgccgacctg cggctgccgg
     1861 atgcctggcg cgtgcgcggc gacgagcgac tgctcgacga actgcgcgcg cagccgcgcg
     1921 tgcgcggggt ggagtgcagt tacggctgag ccgtgtagcg atcgagccct aactcggcgc
     1981 gcgtctcgcg cgagcggatc cgctgctgca agcgctccca gttcgcgcct tcgcgcgcgc
     2041 gatcgtgctc gcggtgccac aggtgcagca gtccggtcgc gaacgcgcct ttcttcagcc
     2101 cgacgccggc attgaccagc cgcaccgcga agtcggagtc ctcgtagccc cagccctgaa
     2161 agctttcgtc gaacccgttc acgcgggcca gatcgcggcg ccacaccgcc atgttgcagc
     2221 ctctgacgcg cggccagcgc gagccgccga gccgacgcag cggacccagc ggcagccgca
     2281 gcagcggcag cacgcggttg atgtcgccgc gccggcgcgc cgcacgccac tgcgacaacg
     2341 tccagcgatg gatcgggacc tgctccgcca gcacgcggcg cgtgaagccc tccgacagca
     2401 gcagccggtt gcccgccacc atccagtgcg gctcggccag cgaccgatgc tgggcgacga
     2461 aatcggccag cggcacgcag tcgccgtcga cgaagaccag atagtcgccg cgcgcttgcg
     2521 cggctgcgcg gttgcggatc gccgcgagcc gaaagccggt gtcctcgtgc cacacgtgcg
     2581 caatcgggat cggcgcgctc ggcgcgatcg actcgaccag cgcgcgcgtg tcgtcgcgcg
     2641 agccatcgtc ggcgaccagc agttcgaaac cgcggtcggt ctgctcgcag aagccggcca
     2701 gcaccgcgcg cagcgcgtcg ggccggttgt aggtcgcgac gatgaccgag accagcattc
     2761 agtcggtctt gtcgcgctgg cgtcgatctg cgagcagcat cgccttcacg taccggtagt
     2821 aggagccctc ggcgttgcta accgcgagca tgaagccgtg cttgccgtcg aggaaaccga
     2881 gcttgaacac gtaggtgcgc aggaacgccg cggcgccatg caggatggcg gtcgccaggc
     2941 ccggcgcggc accgcgctcg gcaagcgttt gcgccgaggc cgacgagtaa tgattgatct
     3001 tgtcgagtac cgcttccatg gtgcggaagc tgtaatgcac gagcggggcg gacagacgcc
     3061 cgattggccg gtcggtgacg agcccttcgt ggacgcgcgt atcggagaag cgcgccgtgc
     3121 cgcgtcggaa cagccgcaca cagtgatccg gccaccaacc gccgtgctgc atgaagcggc
     3181 cgacgtaata cgagcggcgc ggcatttcgt acacgtcaaa gcgcggggcg gcgagcgcgt
     3241 gttcgatctg cgcgcgcaga tcggcgctga cgcgctcgtc ggcgtcgatc gacaggatcc
     3301 actcgccatg acacgcatcg accgcgcggt tcttctgcac gccgaagcct ggccagtcgg
     3361 cttcgatcac gcgcgcgccg tgttgccgcg ccagcgtgac cgtcgcatcg gtgctgccgc
     3421 tgtcaacgac gacgatctcg tcggcgaacg ccacgctggc gaggcagtcc gcgatgttgt
     3481 gcgcctcgtt gcgcgtgatg atcgccaccg acaggcgcgg agcgttgtcc atcagtgagg
     3541 ccggcactcg aagcggaaca ggatctcgag cgcgcgcggc actgcgtcga cgtgcgacgc
     3601 cagctcgcgc agcatcgcgc gcgcctcgac acggcggccg gcgcggtaca ggttcgccgc
     3661 gtgcacgccg ccgtagtggc gccacagcgc gcgatgcgcg gcgaagatcg gctggtggcg
     3721 gcgccacagc gattccgatg cggcgacgtg gaccgacgcc ttcgaggcgc ggctcaggct
     3781 cgtgccggcg tgttcgtgga tgcgcaccag cggctcgcgc aggaacagcg cgcgcgcgcc
     3841 ggcggcgacc gcgcgcatca acaggtcgat atcctccgac acgccgagcg attcatcgaa
     3901 gcccccgagc tgccgcagcg tcgccgtgcg cagcgccagc ccgcgcgacg ccgacgcctt
     3961 gcgcaggaaa tcgatgtcgg cggcgtcggc gtcgaacggc tcggcgcgtt ccgtgccgtc
     4021 ggcataaacg cgcgtgacgc cgggcatgca gaaggatgcc gccggttggc gtgcgaacgc
     4081 agtcacgacg gcgccgaggt actccggttc gtactcgtcg tcgtcatcga ggaaggcgac
     4141 cagcggcgcg gacgcgagcg cgatgccgcg attgcgcgcc gccgccgccc cgctcgccgc
     4201 cgcgttgcgg tggagatgga tgcgcgcgtc ggcgcgatcg atcgcagcgg tggccccggc
     4261 ggccgagccg tcgtcgatca cgatccattc ccagtcggcg tgccgctgcg cgagcagcga
     4321 acgcagtgcg cgcggcagca gtgtcggccg atccctggtc ggggtgacga cacttaccgt
     4381 cggtagactt gcgcgcggtt cagccaccgt ggttctactt cagttgcatc ctgatcctgc
     4441 gaatgctatc cgatggtatc cggcgtgccg cgcgcgttga ccgcgggtga ggccacagcc
     4501 gcgcgggagg cgcgcctgct caccgccgcc cgtgcgtgcg cgctgctcgc gctgattggc
     4561 gcattcgtga agccggtcgc agcgaactgg ggcgcggggc tgatgctgct ggcgttcgcg
     4621 ctgctgccga cggccggcac gcggctgcgt gcgctgctgc gcgaaccgct gccgcgtgcg
     4681 gcgctgctgc tcctggcggt catggcgctg tcgatgctgt ggagttcggc gccttggccg
     4741 gaacggttcg cgcatttctg gagctggcgc acgctgctcg tgctgatcgt gagcctcgcc
     4801 gtgttcgaca gccgcgcctg gaagctcagg ctcgtgctga cgatcgtcgc ggcgacgttg
     4861 gtcggcgccg tcgccgcatg gatcacctgg gcgctcgact atcgcgttta cccgattcat
     4921 cctgcgggca gcgtgctgcg caacggcgtg acgcaaggcc tcgctttcgc ggtcgcggcc
     4981 ttcctcgctg tggtggcggc gctgttcgag cacggcctct cgcggcgatt gcgcatcgcg
     5041 ctgatcggcg cggccgcggt gctggtcgcc agcctgttct tcgtgactgc ggggcgcagc
     5101 gcgcagatcg ggctcgtggt gatgaccgcc gcggcggcat tgttgctgct gcgggggcgc
     5161 gcgcggcgcg tcgcgctgtt cgcgattccg gtcatgtttg ccgccggcgt actgctcgcg
     5221 ccgatggcga aggagcgctt cgttatcggc tggcaggagg ccacgcgcat cgacggtgcc
     5281 gcggacggtg gctcggtcgg gttccgcctg ctgctgtggc gcgagaccgc acggctgatc
     5341 gccgatcgcc cactgctcgg ctacggtagc ggcggcttcg ctgccgctta caacgagcgc
     5401 gtcgagcgcc agtacaccga ctggcgccgc cgccagggca ccgatccgca caaccagtac
     5461 ctgtgggtcc aggtgcgcgc cggtgtgctc ggaacgctcg catttgcctg gtttctgctc
     5521 agcgcggcgc gccagagtgc gccgcaaccc tatcgctcgg ccgcgctcgc gatcctcgcg
     5581 atgtggtgcg ttacgagcct cgccaattcg cacttcgaaa gcttcaacga gggccacatg
     5641 atcgcgctgc tgctcggctg cctgctggca cgcgagagcg atcgcgacct tcaggccgaa
     5701 agcccccgca tcacggcctt ctcgacctcg tcataagccg gccagcctgt cgccgatccg
     5761 accagaatca cgcgcgcacc ccacggccgc gtcgcggtgg gatcggtgac gccgaacacg
     5821 ctgaccagcg gcacgccgac cgccgcggcg agatggcccg acccgctgtc gttggcgacg
     5881 acgagccggc ttgccgccag cagcgcggcg aaagcgccga catcggtctc ggcgagcagc
     5941 gccgcgttcg gaatcgcctg cagcaccgct tcgcgctcac cggggccggg gcaggcgaca
     6001 accgtcacgc cgcgcgcgcg cagcgcatcg gcgagcgcgc cgaaaccgct ccacgccttg
     6061 accttgccgt aatgccggcc gaccgcgacc ggacacagca cgacgtacgg accggcgacg
     6121 ccggcggcgc gcagcagttc gcacgcacgt cgcctggcct cattggtgac gcgcaggttc
     6181 agctcgcccg gcgacgccgg ggcgtgcggc accagttgcg cggcgagccg gtagtagtac
     6241 tcgaccatat gtgtggcgcg gccgatccgc ggccgtgcga ccgcgcggct caacagaagg
     6301 ctgcgcccat cggctgcata accgagcggc cgcagtcccg cgagccggaa ttccagtgcg
     6361 gtcgagaacg aattcgtgaa cagcagtccg cgcccgcccg ggtgtgcgac gcgccacgca
     6421 cgcagcgcgc gcagccgctc agcgcgctgc gccgcgagtg cctgcaccgg ccatgggtac
     6481 gctgcaaaca gcgggtggga ccaaccacaa acc
//
LOCUS       DBNHBY010000348         3697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_697156, whole genome shotgun sequence.
ACCESSION   DBNHBY010000348 DBNHBY010000000
VERSION     DBNHBY010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_697156"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="AC16HB_08580"
     CDS             <1..331
                     /locus_tag="AC16HB_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606888.1"
                     /translation="AHLRRWVVDANGVTEAQGTVFFDTVTGFHARPRLLGDLVTVGVA
                     PEPSSFGDGTPERAQLWATVRGKPGEWIAVGSADARTDAHSIDVFSAHSAQRGVWLKV
                     DADASPR"
     gene            complement(283..1494)
                     /locus_tag="AC16HB_08585"
     CDS             complement(283..1494)
                     /locus_tag="AC16HB_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR5606889.1"
                     /translation="MSFGAHRLDVVDALRAFALAGILQVNIQSFVWGAGDPLGYFPSV
                     PSVSDALVHVLIGTLVSTKFMSIFAFLFGTGFALQWRGLRRRTASLQEAQRAYRRRLA
                     FLLAVGLAHGTLLYHGDILAFYALCGFVLLLFAQARPAPLLRATRNAWLACAGWSIAW
                     IALTESARLALPADEPAQVPATALARLALYTRGGFVDQLAGRIGDFEEGLLSMIAFAT
                     PQIVALFLLGAVAARLGWLAHPERHPRVWRAATWIGLAAIPFSFAGAWLNFDAVRDTP
                     GDPTSFGYALQLVGSLMACLYVALFVTLQAHLARAIAWLAPAGRMPLTNYLVQSVAMG
                     WLLSGWGLGMGAELSRAQLALLGLALVALQLVASRAWIARFGQGPVEALWRAWTYRGL
                     ASASTFSQTPR"
     gene            complement(1491..1988)
                     /gene="coaD"
                     /locus_tag="AC16HB_08590"
     CDS             complement(1491..1988)
                     /gene="coaD"
                     /locus_tag="AC16HB_08590"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5606890.1"
                     /translation="MVIAVYPGTFDPLTRGHEDLVRRAAGLFAKLVVGVADSRAKNPF
                     FTYDERLQIAREVLGHYPNVEVSGFRGLLKDFVREHDARVIVRGLRAVSDFEYEFQLA
                     GMNRYLLPDVETMFLTPSDQYQFISGSIVREIAMLGGDVSKFVFPSVDKWLKAKIAQL
                     GRTPD"
     gene            complement(1991..2686)
                     /gene="rsmD"
                     /locus_tag="AC16HB_08595"
     CDS             complement(1991..2686)
                     /gene="rsmD"
                     /locus_tag="AC16HB_08595"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5606891.1"
                     /translation="MPGGSAKKRAATRAASPRSLATRAPSFGAAPNAVRIIGGIYKRT
                     KIAVADVSGLRPTPDRVRETLFNWIAHLKQDLARARGLDLFAGSGVLGFELASRGAAQ
                     VTLVERDQRLLANLRDLRERLRATNVEIVAGDAFVVAQRLPEAGFDLILLDPPFAAGL
                     NDPALKAARRLVAADGLVYLESATELTDEQAASAGFEVVRGARVGRVHFHLLRPRDAG
                     IIASRGLVAGLGE"
     gene            2693..3625
                     /gene="ftsY"
                     /locus_tag="AC16HB_08600"
     CDS             2693..3625
                     /gene="ftsY"
                     /locus_tag="AC16HB_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5606892.1"
                     /translation="MISPAPEKSGWLARLKKGLERTRVNIVGLFSGGVVDDALFEELE
                     IALIGADVGVATTTELLDALRRRVKLGGLKTPIEVRDALREELTQRLAPCESELKLDA
                     AKPRVMMVAGVNGAGKTTSIGKLAHWLADRHKSVLLAAGDTFRAAAREQLAIWGRRNN
                     VDVIQQASGDPAAVVFDAVGAARARGVDVLIADTAGRLPTQLHLMDELKRVKRALDKA
                     MPGAPHEVLLVLDGTNGQNALAQVRAFDEAIGLTGLVITKLDGMARGGVLAAIARERR
                     DRPLPILFIGVGEKLGDLQPFVAKEFADALLGAP"
BASE COUNT          654 a         1270 c         1257 g          516 t
ORIGIN      
        1 cgctcacctt cggcgctggg tcgtcgacgc gaacggcgtg accgaagcgc aaggcaccgt
       61 cttcttcgat acggtgaccg gcttccacgc tcggccgcgg ctgctgggcg acctggtcac
      121 cgtcggagtc gcgcccgagc cgtcgtcctt cggcgacggc acaccagaac gcgcgcagct
      181 gtgggcgacg gtgcgcggaa aaccgggcga atggatcgcg gtcggcagcg ccgacgcccg
      241 cacggatgcg cattcgatcg acgtcttcag cgcacattcg gctcagcgcg gcgtctggct
      301 gaaggtcgac gcagacgcga gtccgcgata ggtccacgcg cgccacagcg cctcgaccgg
      361 accctgcccg aagcgcgcga tccaggctcg actggcgacg agttgcagcg cgaccagggc
      421 cagtccgagc agcgccagtt gcgcgcgcga tagctcggcg cccattccga gcccccatcc
      481 ggacagcagc catcccatcg caaccgattg cacgaggtag ttggtcagtg gcatccgccc
      541 cgccggcgcg agccacgcga tcgcacgcgc gaggtgcgcc tgcagagtca cgaacagcgc
      601 gacgtacagg caggccatca gcgatccgac aagctgcagc gcatagccga aagaagtcgg
      661 atcgcccggc gtatcgcgaa cggcgtcgaa attcagccat gcacccgcaa aggagaacgg
      721 aatcgccgcc aggccgatcc atgtcgccgc gcgccatacg cgcggatggc gttcgggatg
      781 tgcgagccaa cccagccgtg ctgcgaccgc accaagcagg aacagcgcga cgatctgcgg
      841 cgtggcgaac gcgatcatcg acagcagacc ttcttcgaag tcgccgatgc ggccggccag
      901 ctgatcgacg aatccgccgc gcgtgtacag cgccagccgc gccagcgcgg tggctggcac
      961 ctgtgccggc tcatcggccg gcagcgccaa tcgcgcgctc tcggtcagcg caatccaggc
     1021 gatgctccag cccgcgcatg ccaaccacgc attacgcgtg gcgcgcagca acggcgccgg
     1081 acgcgcttgt gcaaacagca gcagcacgaa accgcacagc gcgtagaacg ccaggatgtc
     1141 gccgtggtac agcagcgtgc catgcgcgag tccgacggcg agaagaaacg cgagtcggcg
     1201 ccgataggcg cgttgcgcct cttggagcga cgcggtgcgc cgacggaggc cgcgccactg
     1261 cagcgcgaac ccggtgccga acaggaacgc gaagatcgac atgaacttgg tcgacaccag
     1321 cgtgccgatc agcacgtgca cgagcgcgtc gctgaccgag ggcacagacg gaaagtagcc
     1381 gagcggatcg cccgcgcccc agacgaacga ttgaatgttg acctgcagga tgccggcgag
     1441 cgcgaacgcg cgcagtgcgt cgacgacgtc gagtcgatgc gcgccgaagc tcaatccgga
     1501 gtacgaccca gctgcgcgat cttggccttc agccacttgt cgaccgacgg gaagacgaac
     1561 ttgctgacgt cgccgccgag catcgcgatc tcgcgcacga tcgaaccgga gatgaactgg
     1621 tactggtccg acggcgtcag gaacatcgtc tccacgtcgg gcagcaggta gcggttcatg
     1681 ccggcaagct ggaactcata ttcgaagtcg ctgaccgcgc gcagcccgcg cacgatcaca
     1741 cgcgcgtcgt gctcgcgcac gaagtccttc agcagcccgc ggaagccgga cacctcgacg
     1801 ttcggatagt ggccgagcac ctcgcgcgcg atctgcagcc gctcgtcgta ggtgaagaaa
     1861 ggattcttcg ctcggctgtc ggccacgccg acgaccaatt tcgcgaacag gcccgccgcg
     1921 cgccgcacga gatcctcgtg gccgcgcgtc agcgggtcga acgttccggg atagacggcg
     1981 atgaccatgc tcattctccg aggcccgcga ccaggccgcg ggacgcgatt attccggcat
     2041 cgcgcggccg cagcaaatgg aagtgcacgc gccccacgcg agcaccgcgc acgacctcga
     2101 acccggcgct tgcggcttgc tcgtcggtca gttcggtcgc gctctcgaga tagaccagtc
     2161 cgtcggcggc gaccagccgc cgcgccgcct tcagcgcggg atcgttcagt ccggccgcga
     2221 acggcggatc gagcaggatc aggtcgaagc ctgcttccgg caaccgctgc gcgacgacga
     2281 acgcgtcgcc tgcgacgatc tcgacgttcg tcgcccgcag ccgttcgcgc aggtcgcgca
     2341 ggttcgcgag caggcgctga tcgcgctcga ccagcgtcac ctgagcagcg ccgcgcgacg
     2401 cgagctcgaa cccgagcacg cccgagccgg cgaacagatc cagcccgcgc gcccgcgcga
     2461 ggtcttgctt cagatgcgcg atccagttga acagcgtctc gcgcacgcgg tcgggcgtcg
     2521 gccgcaatcc agaaacgtcg gcgaccgcaa tcttcgtccg cttgtagatg ccgccgatga
     2581 tgcgcaccgc gttcggcgcg gctccgaatg acggcgcccg cgttgctaga cttcgtggcg
     2641 acgccgccct cgtggcggcg cgttttttcg cagatcctcc tggcatcgat cgatgataag
     2701 tcccgcgccc gaaaagtccg gctggcttgc gcgcctgaag aaagggctgg agcgcacgcg
     2761 cgtgaacatc gtcggcctgt tctccggcgg cgtggtcgac gacgcgctgt tcgaggaact
     2821 cgagatcgcg ctgatcggcg cggacgtcgg cgtggcgacg accacggaac tgctcgacgc
     2881 gctgcgccgg cgcgtgaagc tcggcggact gaagacaccg attgaggtgc gcgacgcgct
     2941 gcgcgaggaa ctgacgcagc gactcgcgcc ttgcgagagc gaactcaagc tcgacgcggc
     3001 gaaaccgcgg gtgatgatgg tcgccggcgt caacggcgcc ggcaagacga cgtcgatcgg
     3061 gaagctcgcg cactggctcg ccgatcgcca caagagcgtg ctgctcgccg ccggcgacac
     3121 gtttcgcgcg gccgcccgcg agcagttggc gatctggggc aggcgcaaca atgtcgacgt
     3181 gatccagcag gcgagcggcg atccggcagc agtcgtgttc gacgccgtcg gcgcggcgcg
     3241 cgcgcgcggc gtcgacgtgc tgatcgcgga cacggccggg cgtctgccga cgcaactgca
     3301 tctgatggac gagttgaagc gcgtgaagcg tgccctcgac aaggcgatgc ccggcgcgcc
     3361 gcacgaggtg ctgctggtgc tcgatggcac caacggccag aacgcgctgg cgcaggtcag
     3421 ggcgttcgac gaggcgatcg ggctgacggg tctcgtgatc accaagctcg acggaatggc
     3481 caggggcggc gttctggcag cgatcgcgcg cgagcgacgc gaccgcccgc tgccgatcct
     3541 gttcatcggc gttggcgaga agctcggcga cctgcagcct ttcgtcgcga aggaatttgc
     3601 cgacgcgctg ctcggcgcac cctgatcact gcactgttgg ccgccctctc ggcctcggcg
     3661 gtgccggccg cttgccgtcg acgatgtgca tcagcgc
//
LOCUS       DBNHBY010000349         3530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_697260, whole genome shotgun sequence.
ACCESSION   DBNHBY010000349 DBNHBY010000000
VERSION     DBNHBY010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_697260"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /gene="glyS"
                     /locus_tag="AC16HB_08605"
     CDS             <1..1885
                     /gene="glyS"
                     /locus_tag="AC16HB_08605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR5606893.1"
                     /translation="AQDKAKNWTAAFLKKLDGLGRGHLAAVAIGTHEGPDALTVESDG
                     KAESVFLKGIAAGQPLFRGLQGALDEALARLPIPKVMSYQLDDGVTTVQFVRPAHRLV
                     ALHGHTPLPVQALGLTGGTKTFGHRFHSEGEIVVHAADTYAEQLEREGMVIASFAARR
                     DRIVALLRKAAAHLNAQPLMPDELLDEVTALVEWPVVYESDFDVDFLAVPPECLILTM
                     QQNQRYFALRDSGGKLLNRFLLVSNLDVNDPSAIVTGNARVVRARLADAKFFFDHDRK
                     QTLESRIPGLANVVYHNKLGSQLERVERITGIAVEIAKALDVDETHVERAARLAKSDL
                     RTLMVGEFPELQGIMGRYYAQHDGEAKDVVDAIEEHYRPRFAGDALPASMVGTCVALA
                     DKLETLVGLFGIGEKPTGDRDPFALRRHALGILRMLIERKLAVGLDRLIAIASREFAS
                     AKAWRESTDDLAAFLYERLRGLLREDGYTANEVEAVVSLAPARLDDIVARLAAVRAFM
                     QMPEAESLAAANKRIGNILKKSPEVPGAFDAGLLFEPAERSLAAAYERLRPTTETLFV
                     RGDYTGMLKSLAPLKQPVDRFFDDVMVNVEDPRLRGNRLALLATLRAQMNRVADLSLL
                     AAG"
     gene            1889..2671
                     /locus_tag="AC16HB_08610"
     CDS             1889..2671
                     /locus_tag="AC16HB_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5606894.1"
                     /translation="MRIASWLRSALHLALLAVTVVPWALVVLAAIVTSQAVRYRLCTR
                     WTWFAIWTARVVCGIRWRVEGWENLPSGPAILLPKHQSTWETFWLPSYLPHQLTFVYK
                     RELHLIPFFGWAMASLDMINIDRSRGQDAFEQVVSQGNRHLREGWWIVIFPEGTRTPP
                     GSTRRYKTGGVRLAMRTNTPVVPIAVNSGECWPRKAFIKTPGEITVVIGRPISPEGKT
                     ADELAALVESWIETEMRRLAPHRYRGPYVPERVAARERSANS"
     gene            2668..3495
                     /locus_tag="AC16HB_08615"
     CDS             2668..3495
                     /locus_tag="AC16HB_08615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5606895.1"
                     /translation="MKRFNAFFDVRQLMLVFDAPAPPLVMTPCPRANHEGRRRIALGH
                     EVFDYRLRRARRRTIGFQIDGGGLTVSAPRWVRLAEIEAAITEKERWIRARLAEWHDW
                     RRAHRLHEMRFADGGLLPYLGGHVTLRLRADAPETRIAETPLTHELHVALPPHVDEPQ
                     VRDAVQAWLQGEAQRVLGERVALLAAPLATRLRGWKLSSARSQWGSCTHDGRIRLNWR
                     LIHFSLAVIDYVVAHELAHLTEMNHSTRFWRTVGELLPGFESARDEIKGVDLGALVI"
BASE COUNT          590 a         1168 c         1220 g          552 t
ORIGIN      
        1 cgcgcaggac aaagcgaaga actggacggc ggcctttctc aagaagctcg acgggcttgg
       61 tcggggccac ctcgccgcag tggccatcgg aacccatgaa gggcccgatg cactgactgt
      121 cgaaagcgac ggcaaggcgg aatcagtctt cctcaagggc atcgcggccg ggcaaccgct
      181 cttccgagga ttgcaggggg cactcgacga agcgcttgcc aggttgccga tcccgaaggt
      241 gatgagctac cagctcgatg acggcgtgac cacggtgcag ttcgtgcggc ccgcgcaccg
      301 gctggtcgcg ctgcatggcc acacgccgct gccggtgcag gcgctggggc tcaccggcgg
      361 caccaagacc ttcggccacc ggttccactc ggaaggcgag atcgtcgtgc acgcggccga
      421 cacatacgcc gagcaactcg aacgcgaagg catggtgatc gcgtcgttcg cggcgcggcg
      481 cgaccggatc gttgcgctgc tacgcaaggc ggcggcgcac ctgaatgcgc agccgctgat
      541 gccggacgaa ctgctcgacg aggtcacggc actggtcgag tggccggtcg tctacgaatc
      601 ggatttcgac gtcgatttcc tcgcggtgcc gcccgaatgc ctgatcctga ccatgcagca
      661 gaaccagcgc tatttcgcgc tgcgggacag cggcggcaag ctgctgaacc gcttcctgct
      721 ggtgtcgaac ctggacgtga acgatccgtc cgcgatcgtc actggcaacg cgcgcgtggt
      781 gcgcgcgcgg cttgcggacg cgaagttctt cttcgaccat gaccgcaagc agacgctcga
      841 gtcgcgtatc ccggggctgg cgaacgtcgt ctatcacaac aaactcggct cgcaactgga
      901 gcgcgtcgag cgcatcaccg gcatcgcggt cgagatcgca aaggcgctcg acgtcgacga
      961 aacacacgtc gagcgcgcgg cgcggctcgc caaatcggac ctgcgcacgt tgatggtcgg
     1021 cgagtttccc gagctgcagg gcatcatggg ccgctactac gcgcagcacg acggcgaagc
     1081 gaaggatgtc gtcgacgcga tcgaggagca ctaccgcccg cgcttcgccg gcgacgcgct
     1141 gccggcgtcg atggtcggca cctgcgtcgc attggcggac aagctggaga cgctcgtggg
     1201 cctgttcggc attggcgaga agccgaccgg cgaccgcgac ccgttcgcgc tgcgccggca
     1261 cgcactcggc atcctgcgca tgctgatcga aaggaagctt gcggtcggac ttgaccgcct
     1321 gatcgcgatc gcatcgcgcg agttcgcctc cgccaaggcg tggcgcgaat cgaccgacga
     1381 ccttgccgcg ttcctgtacg aacggctgcg cggcctgctg cgcgaggacg gctacacggc
     1441 aaacgaggtc gaggcggtgg tgtcgctcgc acccgcgcgg ctcgacgaca tcgtcgcgcg
     1501 gctggcggca gtgcgcgcgt tcatgcagat gcccgaggcc gaaagcctcg ccgcggccaa
     1561 caagcgcatc ggcaacatcc tgaagaagtc gccagaggta cccggcgcat tcgatgccgg
     1621 actgctgttc gagccggcgg aaagatcgct ggcggccgcc tacgagcggt tgcgaccgac
     1681 gacggaaacg ctgttcgtgc gcggcgatta caccggcatg ctgaaatcgc tggcgccgtt
     1741 gaaacaaccg gtcgaccgct tcttcgacga cgtgatggtc aatgtcgagg acccgaggct
     1801 gcgcggcaac cgcctcgcgc tgctcgccac gctgcgcgcg cagatgaacc gcgtggcgga
     1861 tctctcgctg ctggctgcgg gatgaccggt gcggatcgcg agctggctgc gctcggcgct
     1921 tcacctcgct ctgctggcgg tcaccgtggt gccgtgggca ctggtcgtgc ttgccgcgat
     1981 cgtcacgagc caggcggtcc gctacaggct gtgcacgcgc tggacctggt tcgcgatctg
     2041 gaccgcgcgc gtcgtgtgcg gcatccgctg gcgcgtcgaa ggctgggaga acctgccgtc
     2101 cggaccggcc atcctgctgc ccaagcacca gtcgacatgg gaaacgttct ggctgccgtc
     2161 gtatctgccg caccagctga cgttcgtata caagcgcgaa ctgcacctga tcccgttctt
     2221 cggctgggcg atggcatcgc tggacatgat caacatcgac cgcagccgcg ggcaggacgc
     2281 gttcgagcaa gtggtcagcc agggcaatcg gcacctgcgc gaaggctggt ggatcgtgat
     2341 ctttccggaa ggcacgcgca ccccgcccgg atcgacaaga cgctacaaga ccggcggcgt
     2401 gcggctcgcg atgcgcacga acacgcccgt ggtgccgatc gccgtcaaca gcggcgaatg
     2461 ctggccgcgc aaggcattca tcaagactcc gggcgagatc acggtcgtga tcggcaggcc
     2521 gatctcgccc gagggtaaga ccgccgacga gctggctgcg ctcgtcgagt cgtggatcga
     2581 gaccgagatg cgacgcctgg cgccacatcg ctatcgcggg ccgtacgttc ccgagcgcgt
     2641 agccgcgcgc gagcgcagcg cgaactcgtg aagcggttca acgccttctt tgacgtccgc
     2701 cagctgatgc tggtgttcga cgcgccggcg ccgccactgg tcatgacgcc gtgcccgcgc
     2761 gccaaccacg agggtcggcg ccgcatcgcg ctgggacacg aggtgttcga ctaccggctg
     2821 cggcgcgcac ggcgccgcac gatcggattc cagatcgacg gcggcgggct gacggtgagc
     2881 gcgccgcgct gggtacgact ggcggagatc gaagcggcga tcaccgagaa agagcgctgg
     2941 atccgcgcca ggctcgccga gtggcatgac tggcgccgcg cgcatcggct gcacgaaatg
     3001 cgcttcgctg acggcggcct gctgccttac ctcggcgggc atgtcacgct gcgcctgcgt
     3061 gcggatgcgc cggaaacgcg cattgccgag acaccgctca cgcacgagct gcacgtcgcg
     3121 ctgccccctc acgtcgacga gccgcaggtg cgcgacgcag tgcaggcctg gctgcagggc
     3181 gaagcgcagc gcgtgctcgg cgaacgcgtg gcactgctcg ccgcgccgct ggcgacccgg
     3241 ctgcgcgggt ggaaactgtc gtcggcgcgc tcgcagtggg gctcgtgcac gcacgatggc
     3301 cgcatccgcc tgaactggcg gctgatccat ttttcccttg ccgtgatcga ctacgtggtc
     3361 gcgcacgaac tggcgcacct gaccgagatg aaccattcca cgcgcttctg gcggacggtc
     3421 ggcgagttgc tgccggggtt cgagtcagcg cgcgacgaga tcaaaggcgt cgacctcggt
     3481 gcgctggtga tctgagctgg cttggcgcct gcggtcagta ccgaggagac
//
LOCUS       DBNHBY010000350         3947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_20824, whole genome shotgun sequence.
ACCESSION   DBNHBY010000350 DBNHBY010000000
VERSION     DBNHBY010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_20824"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="AC16HB_08620"
     CDS             complement(<1..330)
                     /locus_tag="AC16HB_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606896.1"
                     /translation="MLEIAVVALAVALVVALIALALLRRGALAGVESELRRLQDEQGQ
                     VNERLERELRGAIDAAATATRMETGARFTELQSAINVQTTQANTALAQNLNAYGQTMQ
                     QQLAELTR"
     gene            complement(639..2132)
                     /locus_tag="AC16HB_08625"
     CDS             complement(639..2132)
                     /locus_tag="AC16HB_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR5606897.1"
                     /translation="MQSLRSRALLATVVTANILQGLDLTITNIALATIAGQMSATLNE
                     AAWLLTAYVVGAALGLPLTGFLNDRLGRRRLFIGTVAGFTATSLMCGLSTSLPVLVLA
                     RLLQGFFSAPIAPICNAVLASSYPSEQLPKVLSYFNMSMLIVPILGPALGGFVTEEYG
                     WRWLFLANVPIGCVILLAALRFMDETPRAERLMDWTGFVVLAAALVSLQLVIDHGNDK
                     GLDSPFILACVFTLLAALAMLVVHYASRPAAPILGLAALRDRNYALAILACIPFSIAL
                     WGGQVLQPFLFQQQYGHPPVDAGLLLMPRALGALATMYLYSRIASKFSASTFLLAGGV
                     LASIGCVWMTGMSPDMTGQWMVAPLVLQGLGLGLIVTPIVVMAFMTLPKELAAEASTL
                     YFLVRTVTSAAAIGAISAYTTYGFDRYWGEMRGLIDAMSPAASQYLAPLGLTPGSPEG
                     AAALAAALQKQATMAAFIDSHWVAAFATIALLPLAFALRAGSRPTAH"
     gene            complement(2140..3120)
                     /locus_tag="AC16HB_08630"
     CDS             complement(2140..3120)
                     /locus_tag="AC16HB_08630"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrA/EmrK family multidrug efflux transporter
                     periplasmic adaptor subunit"
                     /protein_id="HIR5606898.1"
                     /translation="MTKVTKLALFGAIAVAALAGFAWTQSAKPTRSTENAYVGADAIP
                     VATQIAGRVTRVHVKPNAAVTAGEPLLDLDERPYRVAIERAQAKLAQARLAAREALAE
                     VSAAEAEVKRVEHQIGQSRTRLARANDLARRGFVSSQGAEDAQAQLKIDQASLDAARA
                     RVVRARTEAYPNVDEHPAVLAALAELRQAELDLEYTRVRAPEAGRATNLRLTVGTQVQ
                     ANAPLFMLVSERGYWVDANFKEGELPGIAPGQRADVELDALPGTRFKGVVESISPGTG
                     AAFALLPPQNATGNWVKTAQRVPVRIRLTELAPGTALPIGASANVTVHVR"
     gene            complement(3214..3876)
                     /locus_tag="AC16HB_08635"
     CDS             complement(3214..3876)
                     /locus_tag="AC16HB_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR5606899.1"
                     /translation="MRIARIVLTITAIGLIAGCANMSEKQKGTATGAGVGAAVGGILG
                     AVTAGGHTGRSAATGAAVGAAVGAAGGYLWSKKMEDQKRAMEQASQGTGVKVAQTADN
                     RLKLDIPADASFDVGRAELKPAMRTVLNKYATTLNEHAVTTIEIIGHTDSTGSDAFND
                     KLSIDRAQSTRDYLVQHGVAASRIAIAGRGEREPVASNDTDAGRAANRRVEIYVAEAA
                     PK"
BASE COUNT          613 a         1390 c         1335 g          609 t
ORIGIN      
        1 gcgcgtgagt tcggcgagct gctgctgcat cgtctggccg taggcgttga ggttctgcgc
       61 cagtgcggtg ttggcctgcg tcgtctggac gttgatcgcc gattgcagct cggtgaaacg
      121 cgcgccggtt tccatgcgcg tggcggtcgc cgcggcgtcg atcgcgccgc gcagttcgcg
      181 ctcgaggcgc tcgttgacct gaccctgctc gtcctgcagg cgccgcagtt cggactcgac
      241 tccggccagc gcgccgcggc gcaggagcgc gagcgcgatc agcgcgacaa ccagcgcgac
      301 cgcgagcgcg acgacggcga tttccagcat ccccactccc cgaatgaagc aggggcgatc
      361 ttagagcttg tccgtgagca attcgtacga tgcgttgccg ccagaaagcc cggatgcaag
      421 gcgcgcggcg caggacagta gccgtgccta cggccaagcc gtgcaacgcc gcagccgggc
      481 tttctggcgg caacccgaag ggacgggtct atatggctcg cattgcgttg ttgctcgtcg
      541 cttatttgga ttcaccaaac agcactcctc gcgcctagca ccgcaagcca tctaggcccg
      601 tcgcacgcac gaattactca cggacaagct ctcagccgtc agtgcgccgt cggcctgctg
      661 cctgcgcgca gcgcgaacgc gagtggcagc agcgcgatcg tcgcgaacgc ggcgacccaa
      721 tggctgtcga tgaacgcagc catcgtcgcc tgcttctgca gcgcggcggc gagcgccgcg
      781 gcgccttcgg gcgaaccggg cgtcagtccc agcggcgcga ggtactggct cgcggccggc
      841 gacatcgcat cgatcaaccc gcgcatctcg ccccagtaac gatcgaagcc gtacgtcgtg
      901 taggccgaga tcgcgccgat cgccgcggcc gacgtgaccg tgcgcaccag gaagtacagc
      961 gtcgaggcct cggccgccag ctccttcggc agcgtcatga acgccatcac gacgatcggc
     1021 gtgacgatca ggcccagccc caggccctgc agcacgagcg gcgcgaccat ccattgcccc
     1081 gtcatgtcgg gcgacatgcc cgtcatccac acgcagccga tgctcgcgag cacgccgccg
     1141 gcgagcagga acgtcgaggc gctgaacttc gacgcgatcc ggctgtacag gtacatcgtc
     1201 gccagtgcgc cgagcgcgcg cggcatcagc agcagtccgg cgtcgaccgg cgggtggccg
     1261 tattgctgct ggaacaggaa cggctgcagc acctgcccgc cccacagcgc gatcgagaat
     1321 ggaatgcagg caagaatggc gagcgcgtaa ttgcggtcgc gcagcgccgc cagcccgagg
     1381 atcggcgcgg ctggccggct cgcatagtgc acgaccagca tcgccagcgc ggcgagcagc
     1441 gtgaacacgc acgccagaat gaacggcgaa tctaggccct tgtcgttgcc gtgatcgatc
     1501 acgagctgca gcgacaccag cgccgcggcc agcacgacga agccggtcca gtccatcagc
     1561 cgctctgcgc gcggcgtctc gtccatgaag cgcagcgcgg ccagcaagat cacgcagccg
     1621 atcggcacat tggcaaggaa cagccaccgc cagccgtatt cctcggtgac gaaaccgccg
     1681 agcgcggggc cgaggatcgg cacgatcagc atgctcatgt tgaagtacga cagcaccttc
     1741 ggcagctgtt cggacgggta cgacgacgcc agcaccgcgt tgcagatcgg cgcgatcggc
     1801 gccgagaaga agccctgcag gaggcgcgcc agcaccagca ccggcagcga ggtcgacagg
     1861 ccacacatga gcgacgtggc agtgaagccg gccaccgtgc cgatgaagag ccgccgccgt
     1921 ccgagccggt cgttcaggaa acccgtcagc ggcagcccga gcgcggcgcc gaccacgtac
     1981 gccgtcagca gccacgcggc ctcgttgagc gtggcgctca tctggccggc gatcgtcgcc
     2041 agcgcgatgt tcgtgatcgt gagatcgagg ccctgcagga tgttcgccgt caccaccgtg
     2101 gcgagcagcg cacggctgcg cagcgactgc atggcaggct tagcggacgt gcacggtgac
     2161 gttggcactg gcgccgatcg gcagcgccgt gccgggcgcc agctcggtca ggcggatgcg
     2221 caccggaaca cgctgcgcgg tcttgaccca gttgccggtc gcgttctgcg gcggcagcag
     2281 cgcgaacgcc gcgccggtgc cggggctgat gctttcgacc acgcccttga agcgcgtgcc
     2341 cggcagcgcg tcgagttcga cgtcggcgcg ctgccccgga gcgattcccg gcagctcgcc
     2401 ttccttgaaa ttcgcatcga cccagtagcc acgctccgac accagcatga acagcggtgc
     2461 attcgcctgc acctgcgtgc ccacggtcag gcgcaggttg gtggcgcgcc ccgcttccgg
     2521 cgcgcgcacg cgcgtgtact cgagatcgag ttcggcctgc cgcagttcgg ccagcgcggc
     2581 gagcacggcg ggatgctcgt caacgttcgg atacgcctcg gtgcgcgcgc ggaccacacg
     2641 ggcgcgcgcc gcgtcgaggc tcgcctggtc gatcttgagc tgtgcctgcg cgtcctccgc
     2701 accctgcgac gatacgaagc cccgtcgcgc caggtcgttg gcgcgtgcca gccgcgtgcg
     2761 cgactggccg atctggtgtt cgacgcgctt gacctccgct tcggccgcgg acacctcggc
     2821 gagcgcttcg cgcgccgcca ggcgtgcctg cgccagcttg gcctgcgcgc gctcgatcgc
     2881 gacgcggtag ggccgctcgt cgaggtcgag cagcggctcg cccgccgtca ccgcggcgtt
     2941 gggcttcacg tgaacccgcg tgacgcgacc cgcgatctgc gtggccaccg gaatcgcgtc
     3001 ggcgccgacg tacgcgttct cggtggaacg agtgggcttg gcagactggg tccaggcgaa
     3061 gcccgcgagc gccgccacgg caatcgcgcc gaagagcgcg agcttcgtga ccttcgtcat
     3121 gtccccccct gcaatttctt gttcgccgcc tgcaagcggc aggcggcaag gatagcctcg
     3181 ccgcccggtg ctgtgaaccg tgaaccggcg cgactacttc ggcgctgcct cggcgacgta
     3241 gatctcgacg cgccggttgg ccgcgcggcc cgcatcggtg tcgttgctgg cgaccggttc
     3301 gcgctcgccg cgccccgcga tcgcaatgcg cgacgccgcc acaccgtgct gcaccaggta
     3361 gtcgcgcgtc gactgcgcgc ggtcgatcga cagcttgtcg ttgaaggcgt cggatccggt
     3421 cgagtcggtg tggccgatga tctcgatcgt cgtcaccgca tgctcgttca gcgtcgtcgc
     3481 gtacttgttc agcacggtgc gcatggccgg cttcagttcc gcacggccga catcgaacga
     3541 cgcgtcggcc gggatgtcga gcttcaggcg gttgtccgcc gtctgcgcga ccttgacgcc
     3601 ggtgccctgc gacgcctgct ccatcgcgcg cttctggtcc tccatcttct tgctccagag
     3661 gtagccgccg gcggcgccga ccgcggcgcc gaccgcggcg ccggtcgccg cgctgcgccc
     3721 ggtgtgtccg cctgcggtca ccgcgccgag gatgccgccc acggccgcgc cgacgccggc
     3781 gccggtggcc gtgcccttct gcttctccga catgttcgcg cagccggcga tcagcccgat
     3841 cgcggtgatg gtgagaacga ttcgtgcaat tcgcattgaa gctcccatcg ttgcaaggat
     3901 gaatccattc tggaaagtga atgccgcgtg cggcactccg caaaaac
//
LOCUS       DBNHBY010000351         3135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1509971, whole genome shotgun sequence.
ACCESSION   DBNHBY010000351 DBNHBY010000000
VERSION     DBNHBY010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1509971"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16HB_08640"
     CDS             complement(<1..184)
                     /locus_tag="AC16HB_08640"
                     /inference="COORDINATES: protein motif:HMM:NF053443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606900.1"
                     /translation="MHDRGYLRQPAIRGHTIVFVCDDDLWRVDADGGIARRLTAGVGE
                     PSTPALSPQGQWIAYVG"
     gene            complement(269..997)
                     /locus_tag="AC16HB_08645"
     CDS             complement(269..997)
                     /locus_tag="AC16HB_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5606901.1"
                     /translation="MATDALYRREAQRTECDAVVTQVHEEGVELDRTVFYPLGGGQAG
                     DTGALVLDDGTRLRVTDARKSRRDGATPDDTLHVLDATVDWKPRLAPGIRVRAQIDWE
                     RRHRHMRFHTATHLLCAIVKELVDGCSITRDYARLDFAMTAPLDKERVQRELERLIAE
                     AHTVTYEWISDEELLANPQLVKSMSVSPPIGFGRVRLVRVRDVDLQPCGGTHVSNTSE
                     IGSVRVAKIEKKSARTRRVVLEFT"
     gene            complement(1003..2625)
                     /locus_tag="AC16HB_08650"
     CDS             complement(1003..2625)
                     /locus_tag="AC16HB_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HIR5606902.1"
                     /translation="MPKRVRMSAVDTAWLRMDSPGNLMMIVGVDLFEGPCDLPRLQRL
                     LQAHLLQYRQFRSRVVQDPSGAWWEEDENFDIDQHLVRIGLPGRGGKEELQKLVAQLA
                     GQALDPLKPLWQFHLVENYDGGHALIVRIHHCIADGIALIGVVMSMTATDPAAEDAVP
                     APRRRREGDADNPIEQWLRPFTDATVRALDRTGTLAAKVLHASARALDDPDVASEAAT
                     EYARIATQVGRDVATLALMDSDTPTRLKGRPGGSKVVAWNEPLPLADVKAVGKALGCS
                     INDVLLSCAAGALRGYLLERGDTLAHAELRAMVPVNLRPPGKHHSLGNRFGLAPLLLP
                     IGIANPIERVLAVRERMNEMKGGYLPVLAMGVLGLAGMMPRAVQAPLLDIFQRKATAV
                     MTNVPGPQQAIFMAGARLKQMMFWVPQSGDIGVGVSILSYDGGVQFGLVTDKRLCDDP
                     QKIIDRFAPEFEQLVYAVLLMPWDKGVDPQVAERALNATEALAGAVLNLRERDDVALA
                     HGNGAGAAAAQDESARPKRRRSGLAAVRAAAH"
     gene            2732..2989
                     /locus_tag="AC16HB_08655"
     CDS             2732..2989
                     /locus_tag="AC16HB_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HIR5606903.1"
                     /translation="MSDLKAKFEKAVADSKNLAERPDNTTLLKMYALYKQATAGDIEG
                     KRPGFTDMVGRAKWDAWNEIKGTTANDAMKQYVALVEDLKA"
BASE COUNT          490 a         1097 c         1045 g          503 t
ORIGIN      
        1 gcccgacgta agcgatccac tgcccttgcg gcgagagcgc cggcgtcgac ggctcgccga
       61 cgccggcggt cagccggcgc gcgatgccgc catccgcatc gacgcgccac aggtcgtcgt
      121 cgcagacaaa cacgatcgtg tggccgcgta tcgcgggctg gcgcagatag ccgcggtcgt
      181 gcatggtcga aaatccggtt gtcgttggca gtcgcgcgga ttctggcaac gccgaccgga
      241 gtgtcaaggc aaatattcgc gtggccgttt acgtgaattc cagtacgacg cgccgggtcc
      301 gcgcactctt cttttcaatc ttcgcaaccc ggactgaacc gatctcggac gtgttgctca
      361 cgtgcgtgcc gccgcacggc tgcaggtcga catcgcgcac gcgcaccagc cgcacgcggc
      421 cgaagccgat cggtgggctg acgctcatcg acttgacgag ctgcggattc gcgagcagtt
      481 cctcgtcgct gatccactcg tacgtgaccg tgtgtgcctc ggcaatcagc cgttccagtt
      541 cgcgctggac gcgctccttg tcgagcggcg cggtcatcgc gaagtcgagc cgtgcatagt
      601 cgcgcgtgat gctgcagccg tcgaccagct ctttcacgat cgcgcacaag aggtgcgtcg
      661 cggtgtgaaa gcgcatgtga cgatggcgtc gctcccagtc gatctgcgcg cgcacgcgga
      721 tgcccggcgc gagccgcggc ttccagtcga ccgtggcatc gagcacatgc agcgtgtcgt
      781 cgggcgtggc gccgtcgcgg cggctcttgc gcgcgtccgt cacccgcagg cgggtaccgt
      841 cgtccagcac cagcgcgcca gtgtcccccg cctgcccgcc cccgagcgga tagaacaccg
      901 tgcgatcgag ttcgacgcct tcctcgtgca cctgggtgac cacggcatcg cactcggtgc
      961 gctgcgcttc ccggcgatac agcgcgtcgg tggccatgtg cgtcagtgcg cggcggcgcg
     1021 gaccgcggcc aagccggagc gccgccgttt cggccgtgcc gactcgtcct gcgccgccgc
     1081 ggccccggcg ccgttgccat gcgccaacgc cacgtcgtcg cgctccctga gattcagcac
     1141 agcgcctgcc agcgcctcgg tcgcgttcag cgcgcgctcg gcgacctgcg ggtcgacgcc
     1201 cttgtcccac ggcatcagca gcaccgcgta gacgagctgt tcgaactcgg gcgcgaaacg
     1261 atcgatgatc ttctgcgggt cgtcgcaaag tctcttgtcg gtgacgaggc cgaactgcac
     1321 gccgccgtcg tacgacagga tcgacacgcc gacgccgatg tcacccgatt gcggcaccca
     1381 gaacatcatc tgcttgagcc gcgcgccggc catgaagatc gcctgctgcg gccccggcac
     1441 gttggtcatc accgcggtgg ccttgcgctg gaagatgtcg agcagcggcg cctgcaccgc
     1501 gcgcggcatc atgccggcca gtccgagcac gcccatcgcc agcaccggca ggtagccgcc
     1561 cttcatctcg ttcattcgct cgcgcactgc cagcacacgc tcgatcgggt tcgcgatgcc
     1621 gatcggcaac agcaggggcg ccagtccgaa gcggttgccg agcgaatggt gcttgccggg
     1681 cggacgcagg ttgaccggca ccatcgcgcg cagttccgcg tgcgccaggg tgtcgccccg
     1741 ctcgagcaga tacccgcgca gcgcgcccgc ggcgcacgac agcagcacat cgttgatcga
     1801 gcagccgagt gccttgccga cggccttgac gtcggccagc ggcaacggct cgttccacgc
     1861 cacgaccttc gatcccccgg gccgaccctt cagccgggtc ggtgtgtcgc tgtccatcag
     1921 cgcaagcgtg gcgacgtcgc ggccgacctg cgtggcgatg cgcgcatact cggtcgcggc
     1981 ctcgcttgcc acgtccggat cgtcgagcgc ccgcgccgag gcgtgcagca ccttggcggc
     2041 aagcgttccg gtgcggtcga gggcccggac cgtcgcgtcg gtgaacggcc gcagccactg
     2101 ctcgatcggg ttgtccgcgt cgccctcgcg ccggcggcgc ggcgccggta ccgcatcctc
     2161 ggcggcggga tcggtcgccg tcatgctcat cacgacgccg atcagcgcga tgccgtcggc
     2221 gatgcagtga tggatgcgca cgatcagtgc gtgcccgccg tcgtagttct cgaccaggtg
     2281 gaactgccac agcggcttca gcggatcgag tgcctgcccc gccagctggg caaccagctt
     2341 ctgcagttcc tccttgccgc cgcggccggg caggccgatc cgcaccaggt gctgatcgat
     2401 gtcgaagttc tcgtcctcct cccaccatgc gccgctcggg tcctgcacga cgcggctgcg
     2461 gaactggcgg tactgcagca ggtgcgcctg caacagccgc tgcaggcgcg gcaggtcgca
     2521 cggcccctcg aacaggtcga cgccgacgat catcatcagg ttaccgggcg aatccatgcg
     2581 cagccacgcg gtgtccacgg cagacatgcg tacgcgcttg ggcatcgggg ctctccagaa
     2641 tgcgatcggc cggtcgcgac cggggcgtgc agtctgccac cgtcgcattt cctatgctag
     2701 ctttgcgcca cgcgcattcc cggaggagac aatgagcgac ctgaaggcga aattcgagaa
     2761 ggccgtcgcc gattcgaaga acctggcgga acgccccgac aacactacgt tgctgaagat
     2821 gtacgcgctg tacaagcagg cgactgccgg cgacatcgaa ggcaagcgcc ctgggttcac
     2881 cgacatggtc gggcgcgcca agtgggacgc atggaacgag atcaagggca cgacggccaa
     2941 cgacgcgatg aagcagtacg tcgcgctggt cgaagacctg aaggcttgaa gcagggagcg
     3001 cggtccgacg gcccgccgcg cgggccgtcg tcgttttcgg aggggtggag caaacagatg
     3061 agagcggcaa tcttcgcact ggccctggcg ttcgggacac cgctggcgag cgccggcacc
     3121 gtcacggtca tcact
//
LOCUS       DBNHBY010000352         5556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_20884, whole genome shotgun sequence.
ACCESSION   DBNHBY010000352 DBNHBY010000000
VERSION     DBNHBY010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_20884"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="acuI"
                     /locus_tag="AC16HB_08660"
     CDS             complement(<1..825)
                     /gene="acuI"
                     /locus_tag="AC16HB_08660"
                     /EC_number="1.3.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acrylyl-CoA reductase (NADPH)"
                     /protein_id="HIR5606904.1"
                     /translation="MFKAIVLTRDNDQFNATLRDLDDDALQAATAGADVTLAVEYSTI
                     NYKDALALTNKSPVVRKWPMVPGIDAAGTVSASADPRWKIGDRVILNGWGVGETHWGG
                     LAQNARLKGGWLVALPDGLTARQAMSIGTAGYTAMLCCLALEEQGIGKTSGDILVTGA
                     TGGVGSIALAILSRWGWRTVASTGKLAEAGYLKSLGAADVIDRTELSNPGKPLQKERW
                     AAAIDAVGSHTLANACAQIRYGGVVAACGLAQGMDFPATVAPFILRGVRLIGVDSVM"
     gene            977..4012
                     /locus_tag="AC16HB_08665"
     CDS             977..4012
                     /locus_tag="AC16HB_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5606905.1"
                     /translation="MSTVIPLRQSAVESSLKQAAFDHLALPTICFNASAQILTANGAA
                     AALIGKPVDIVIGRHLAEVLAYPRYRDVTRRWVRFWDRIERDGRVSGQAKVPLGDGRR
                     VAIQFDAELLRFEQESVAAVTLTDISARRDDELARRRDLARTAAIAQASGEIGILIGA
                     DRRVISISAAALTATGVNSGDAIGVPFEYLLDDTAANEFAQAVDRMTAQGDASPLRFE
                     WRLRLSGQGSGRWMLGTLTNRLRDPMVQAIVICARDVTEARETKRRMRLLERRLRSFA
                     EHAADALMVLDGEGLIRYQSAAIQKSLGVKPDSTIGKSGVSLVLEEDQPMLHEAIGAA
                     ARDGSSRRTYSCLVRFAHADGTLRRLWISVRNLMSDATIGGLLVTARDVTGAALACGA
                     EAVRQARRLEYRERLLELAIQTRADFAHSLSRALRVIAETLRAGTVSFWLHEQSPPTL
                     RCEACFDARSDRFLRDWVGTEFPAAVYADYFAQLRDRQPVVVADIGTSSLLAPLVADP
                     RWAGLMATLDVPVLLDGDVHGVLRVQNPEPRSWDEDEINFVTTGALLIALAMEASQRQ
                     EAETRIEQLAWYDSLTGLPNRNLLRETMRDMIMTAANRRRRIAVMLIDLDRFKDVNDT
                     LGHLVGDALIKSAAQIIKDIVVERGMVARLGGDEFVVVLNDFEHRQEVALLAARIAQS
                     LHRTDLVPNVDTQVSASIGIALFPEHGREMSTLLKNADAGMYQAKRDGRNQFSFFNPI
                     RYETAAREVQLGIQMLKALQSEQPQFVLEYQPQVQMNTGRVVGLEALIRWQHPTYGRL
                     TPDRFISVAEVSGLSERITRWLINDVCAQIVRWREKQPSFDIPISINVAGREMGSASL
                     PIIVRSALAKHGIEPHMITFEITERTLVRESEVNNDVLNELATLGVGLVLDDFGTGYS
                     MLAYLKRMPIKALKIDQSFIEGVPNDADSCAIVHAMLAVARHFRLKVVAEGIETPEQV
                     EYLRNIGCEYAQGFYYSRALPPQTILEYIEFGPGDVSAH"
     gene            4027..4803
                     /locus_tag="AC16HB_08670"
     CDS             4027..4803
                     /locus_tag="AC16HB_08670"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR5606906.1"
                     /translation="MNYENILVERRDRVGLITLNRPKALNALNDALMDELGGALLAFD
                     ADESIGAMVITGSPKAFAAGADIGAMREWSYMDVFRSDYISRNWETMRRVRKPVLAAV
                     AGYALGGGCELAMMCDIIIAAESARFGQPEIKLGIIPGAGGTQRLPRAVGKAKAMDLA
                     LTARTMDAAEAERAGLVSRVVADDKLLDEALGAAAAIAQFSLPAVMMAKECINRAYEA
                     PLSEGLLFERRLFHSLFATEDQKEGMSAFVEKRAVRFRHR"
     gene            5151..>5556
                     /locus_tag="AC16HB_08675"
     rRNA            5151..>5556
                     /locus_tag="AC16HB_08675"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          976 a         1741 c         1897 g          942 t
ORIGIN      
        1 catcactgaa tcgacgccga tcagacgcac gccgcgcagg atgaagggtg cgacggtcgc
       61 cgggaaatcc attccctgcg ccagtccgca ggcggcgacc acgccaccat accgtatctg
      121 tgcacacgca ttggccagcg tgtgcgagcc gactgcgtcg atcgccgcgg cccagcgctc
      181 cttctgcagc ggcttgccgg ggtttgacag ctcggtgcga tcgatcacgt cggcggcgcc
      241 gagcgatttg aggtaaccgg cttcggcgag cttgccggtc gacgcgacgg tgcgccagcc
      301 ccagcgcgac aggatcgcga gcgcaatgga acccacgccg ccggtggcgc cggtgacgag
      361 gatgtcgccg ctcgtcttgc cgatgccctg ttcttcgagc gcgagacaac acaacatcgc
      421 cgtatagccg gcggtgccga tcgacatcgc ctgtctcgcg gtcaggccat cgggcagcgc
      481 cacgagccag ccgcccttga gtctcgcatt ctgggcgagc ccgccccaat gcgtctcgcc
      541 caccccccag ccattcagga tcacgcgatc gccgattttc caccgcggat ccgcgctcgc
      601 gctgaccgtg ccagccgcgt cgatgccggg caccatcggc catttgcgta cgaccggcga
      661 cttgttcgtc agcgccagtg cgtccttgta gttgatcgtc gagtactcga cggcgagcgt
      721 gacgtcggca ccggcggtcg cggcctggag tgcgtcgtcg tcaaggtcgc gcagggttgc
      781 attgaactgg tcgttgtccc tcgtgagcac gatggccttg aacatgcgag cctccttcaa
      841 atgcggccag tgtagccatg acgagagcag gcgccggcca cctgcacatc attggtggca
      901 caatcacaga cgtcgccgtc cggcgcggtc cgcgcgatcg cggcggacgc gccgccagcg
      961 caggagacag catcgcgtgt caaccgtcat cccgctacgg cagtcggccg tcgagtcatc
     1021 gctgaagcag gcggcgttcg atcatctcgc gctgccgacg atctgcttca atgcctcggc
     1081 gcagatcctg acggccaacg gcgcggcggc ggcgctgatc ggcaaaccgg tcgacatcgt
     1141 gatcggccgc cacctcgccg aagtgctcgc ctatccgcgc tatcgcgacg tgacgcgccg
     1201 ctgggtccgt ttctgggatc ggatcgaacg cgacggacgc gtttcggggc aggccaaggt
     1261 tccgctcgga gacgggcggc gcgtcgcgat ccagttcgat gccgagttgc tgcgcttcga
     1321 acaggaatcg gtggcggcag tcacgctgac ggacatcagc gcgcgccgcg atgacgaact
     1381 cgcgcggcgg cgcgatctgg cccgcacggc tgcgattgca caggcctcgg gcgagatcgg
     1441 catcctgatc ggcgccgatc ggcgggtcat ctcgatcagt gcggcggcgt tgacggcgac
     1501 cggcgtgaac tcgggcgacg ccattggcgt gccgttcgag tatctgctcg acgacacggc
     1561 agcgaacgag tttgcgcaag cggtcgaccg catgaccgcg cagggtgatg cgagcccgct
     1621 gcgcttcgag tggcggctgc ggctgtcggg gcagggcagc ggacgctgga tgctcggcac
     1681 gctgacgaac cggctgcgtg atccgatggt gcaggcgatc gtgatctgcg cccgcgacgt
     1741 gaccgaggcg cgtgaaacca agcgtcgcat gcggctgctc gagcggcgcc tgcgctcgtt
     1801 tgcggagcac gcggccgatg cgttgatggt gctcgatggc gaagggctga tccggtacca
     1861 atcggcggcg atacagaagt cgctcggcgt gaagccggac tcgacgatcg gcaagtccgg
     1921 cgtgtcgctc gttctcgagg aagatcagcc gatgctgcac gaggcgatcg gcgcggcagc
     1981 acgcgacggc tcgtcgcggc gaacatacag ttgcctggtc cgttttgcgc acgccgacgg
     2041 cacgctgcgg cgactgtgga tctccgtgcg caacctgatg tcggatgcga ccatcggcgg
     2101 actgctggtg accgcgcgcg atgtcaccgg cgccgcgctg gcgtgtggcg ccgaggcggt
     2161 gcggcaggcg cgacggctcg aataccgcga gcgcttgctc gagctggcaa tccagacgcg
     2221 ggcggacttc gcgcattcgc tgtcgcgcgc gctgcgggtg attgccgaga cgctgcgcgc
     2281 gggcacggtg agtttctggc tgcacgagca atcaccgccg acactgcgct gcgaagcctg
     2341 cttcgacgcg cgcagcgatc gatttctgcg tgattgggtg ggcaccgaat tcccggccgc
     2401 ggtgtacgcg gactacttcg cgcaactgcg cgatcggcag ccggtcgtgg tcgccgacat
     2461 cggcacgtcg tcgctgctgg caccgctggt tgcagacccg cgctgggccg gactgatggc
     2521 cacgctcgat gtgccggtct tgctcgacgg cgacgtgcac ggcgtgctgc gcgtgcagaa
     2581 tcccgagccg cgcagctggg acgaggacga gatcaacttc gtcacgacgg gcgcactgct
     2641 gatcgcactg gcgatggagg cctcgcagcg gcaggaggcg gagacccgga tcgagcaact
     2701 cgcctggtat gactcgctca ccgggctgcc caaccgcaac ctgctgcgcg agacgatgcg
     2761 cgacatgatc atgacggcgg ccaaccggcg ccggcgcatc gcagtgatgc tgatcgacct
     2821 ggaccgcttc aaggatgtca acgacacgct cggccacttg gtcggcgacg cgctgatcaa
     2881 gtcggcggcg cagatcatca aggacatcgt ggtcgagcgc ggcatggtcg cgcgactcgg
     2941 cggagacgag ttcgtggtgg tgctgaacga cttcgagcat cgacaggaag tcgccctgct
     3001 cgccgcgcgg atcgcacagt cgctgcaccg aaccgacctg gtgccgaacg tggacaccca
     3061 ggtgtcggca tcgatcggca tcgcgctctt cccggagcac gggcgcgaga tgagcacgct
     3121 gctgaagaat gccgacgccg gcatgtacca ggccaagcgc gacggacgca accagttcag
     3181 cttcttcaat ccgatccgct atgaaacggc ggcgcgcgaa gtgcagctcg gcatccagat
     3241 gctcaaggcg ttgcagtcgg agcagccgca gttcgtgctc gagtaccagc cgcaggtgca
     3301 gatgaatacc gggcgcgtcg tgggcctgga ggcgttgatc cgctggcagc accccaccta
     3361 cggccggctg acgcccgacc gtttcatcag tgtggccgag gtgagcggcc tgtcggagcg
     3421 catcacgcgc tggctgatca acgacgtgtg cgcgcagatc gtgcgctggc gcgagaagca
     3481 gccgagtttc gatatcccga tctcgatcaa cgtcgccgga cgggaaatgg gctcggccag
     3541 cctgccgatc atcgtgcgca gcgcactcgc caaacacggc atcgagccgc acatgatcac
     3601 gttcgagatc accgagcgca cgctggtgcg cgagagcgag gtcaataacg acgtcctgaa
     3661 cgaactggcc acgctcggcg tcggcctcgt gctggacgac ttcggtaccg gctactccat
     3721 gctcgcgtac ctgaaacgga tgccgatcaa ggcgttgaag atcgaccagt cgttcatcga
     3781 gggcgtgccg aacgacgccg acagctgcgc gatcgtgcac gcaatgctgg cggtcgcgcg
     3841 ccacttccgg ctcaaggtcg tggcggaagg catcgagacg cccgaacagg tcgagtatct
     3901 gcgcaacatc ggctgcgagt atgcgcaggg tttctactat tcgcgcgcgc tgccgccgca
     3961 gacgattctc gagtacatcg agttcggtcc gggcgacgta tccgcgcact gatcgcggag
     4021 ttgcagatga attacgagaa catcctggtc gagcggcgcg atcgcgtcgg actgatcacg
     4081 ctgaatcgtc ccaaggcgct caatgcgctg aacgatgcgc tgatggacga acttggcggc
     4141 gcgctgctcg cattcgacgc tgacgagtcg atcggcgcga tggtcatcac gggctcgccc
     4201 aaggccttcg ccgcgggcgc ggacatcggc gcgatgcgcg aatggagcta catggacgtt
     4261 ttccgcagcg actacatcag tcgcaactgg gagaccatgc ggcgcgtacg caagccggtg
     4321 ctcgcggccg tcgccggcta cgcgcttggc ggcggctgcg aactggcgat gatgtgcgac
     4381 atcatcattg ccgcggagag cgctcgcttt ggacagccgg agatcaagct cggcatcatc
     4441 ccgggcgcgg gcggcacgca gcgtctgcca cgcgcagtcg gcaaggccaa ggcgatggat
     4501 ctcgcgctta cggcgcggac catggatgct gcggaggcgg agcgtgctgg gctggtctcg
     4561 cgcgtggtgg cggacgacaa actgctggat gaggcgctcg gcgcggccgc cgccatcgct
     4621 caattctcgc tgccggcggt catgatggcc aaggaatgca tcaatcgcgc gtacgaggca
     4681 cctttgtccg agggcttgct gttcgagcgc cggctatttc actcgctttt tgcgaccgag
     4741 gatcagaagg aaggcatgtc ggcctttgtc gagaagcgcg cggtgcgctt ccggcatcgc
     4801 tgaggctatg tggccgaaca aggacttgca ggtgctcgag gggcgcctcg aaattcgggc
     4861 ggctgctcaa cttgacacct gtttattgcg tccgcataat ctcacctctt cgctgcttgc
     4921 cgacgtgtgc gacgcacaaa gcgtcaggca gcctgctctt taacaatcga aacagccgat
     4981 aagtgtgggc gctggatgag agcgcagcca cggcggccgg cctcacgccg gatgtcgtgg
     5041 taatgctcga tttatctcgt gtctcacacg gaagtcaatg ggaagtcttt cgagatttcc
     5101 ctcaattccg cgagtgagcg aacgtacacg gttacgtgtg cgaaggattt gaactgaaga
     5161 gtttgatcct ggctcagatt gaacgctggc ggcatgcctt acacatgcaa gtcgaacggc
     5221 agcacggggg caaccctggt ggcgagtggc gaacgggtga gtaacgcatc ggaacgtgcc
     5281 ctgtagtggg ggatagcccg gcgaaagccg gattaatacc gcatacgacc tacgggtgaa
     5341 agcgggggac cgcaaggcct cgcgctactg gagcggccga tgtccgatta gctagttggt
     5401 ggggtaacgg cccaccaagg cgacgatcgg tagctggtct gagaggacga ccagccacac
     5461 tgggactgag acacggccca gactcctacg ggaggcagca gtggggaatt ttggacaatg
     5521 ggcgcaagcc tgatccagcc atgccgcgtg cgggaa
//
LOCUS       DBNHBY010000353         5740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_629925, whole genome shotgun sequence.
ACCESSION   DBNHBY010000353 DBNHBY010000000
VERSION     DBNHBY010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_629925"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="AC16HB_08680"
     CDS             complement(<1..1956)
                     /locus_tag="AC16HB_08680"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR5606907.1"
                     /translation="MLRPVRFSLRFIVPLFIAMAGLAYFAVPLVDTLTLRWFVRDLDI
                     RSNLIGRTLGDVLIEQVQARNWGRVDALLDRATSDERLFALAVCSPEGRMLRHTRTLP
                     AAIDCSAVGRLSAGGSEVLNLPQGPVHLAVSELRDGETTVGALLIVHDMSFVTRRSED
                     TRKYVLLTFTALGVAVGLISVAVALLSWRGWMAGVRAMLRGEGVFRPFSHPRPELQPL
                     VGDLRALLREIQMEQQLHDDMTLQWTPDSLRALLREQLSGDEVIVVSNREPYIHVRVA
                     NGVQVQRPASGLVTAVEPVMRACSGTWIAHGSGSADRQTADRRGHLRVPPNEPAYTLR
                     RIWLTREEEQGYYYGFSNEGLWPLCHIAHVRPVFRSDDWVHYQHVNERFAAAVVDEAT
                     TSDPVVLVQDYHFALLPALIRERLPKATIITFWHIPWPNPESFGICPWREEILRGLLG
                     STILGFHTRYHCNNFLQTVDRFLEARIEHESSTVHAGGHLTQVESYPISIAWPAAAQS
                     PADECARRLRSRFGLDPEHRIGLGVDRLDYTKGISERLRAVERLFEQHPEWIGRFTFV
                     QIAAPSRSSLDEYQRFEAEVRAIAARINAKYGDAAYAPVRLLIEHHDSDSVREHYRGC
                     DVCMVTSLHDGMNLVAKEFVAARDDERG"
     gene            complement(2174..3958)
                     /locus_tag="AC16HB_08685"
     CDS             complement(2174..3958)
                     /locus_tag="AC16HB_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5606908.1"
                     /translation="MADRGLELAAIGNSRIGALIDTRASIVWMCVPRFDGDPVFCALL
                     DSDSALGRFDIELDHGASIEQAYERNTPILRSVLRDANGGAVELIDFAPRFYQHERTF
                     APISIVRIVRRVAGRPRVRVRFAPRCDYGASAAPIAFGSHHIRAQVPAYPLRLTTDAP
                     ITSLIEGRPLLVDDTLTFVLGPDETLQGNPHEVGRHLLEETQRYWRRWVRNLALPFEW
                     QDAVIRAAITLKLNTFDDTGGVIAALTTSIPEAPHTARNWDYRYCWLRDAYFVLNALN
                     RLGATGSLERYLHYIENVIADAADAQLQPVYSLAGAAELAESEMPALAGYRGMGPVRL
                     GNLAFQQRQNDVYGAAILGVSHAFFDQRLDRRGDAALLRQLERLGERAFACYATPDAG
                     IWEYRGRQSVHTFSALMCWAACDRLARIATASGESSSSTGWRARATEIRAAIDAQAWD
                     ANRRTYVSEFGGDALDASLLLMGELGYLAPGDPRLRGTVEAVERQLRRGNFLLRYDRP
                     DDFGAPESAFLICTFWWIQALALLGEHTRARDEFERLLACRNRFGLLSEDIDVASGEL
                     WGNFPQTYSMVGIVMCAMRLSARWDDAF"
     gene            complement(3961..4944)
                     /locus_tag="AC16HB_08690"
     CDS             complement(3961..4944)
                     /locus_tag="AC16HB_08690"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR5606909.1"
                     /translation="MYCSLLPALDDASDWVLADIGGTNARFACWHASAGRTTQRQLEN
                     AAFGGLHELLAAYLAEVQRPVKRALLALAMPVTGDDLTFTNRDWSVAAPKLAAQLGFE
                     RLVIVNDFVAAAAGAVGLDAGFVMVQQGVAQGPMRVVLGPGTGLGVAGVLGGGVPARI
                     IESEASHMSFAAIDDESLRVLHAAQQRWGRVSWERLLCGSGLAWLDAFMHGRREPDAP
                     ARVAQRAQAGDAHALAAARWFSRALGSFAGDVCLALRASGGVYLAGGVLAGLGPAFER
                     AAFREGFADKGRFRALLEEVPCWRVDAGDLALHGLADIARGRIGAPGVLVD"
     gene            complement(5017..5499)
                     /locus_tag="AC16HB_08695"
     CDS             complement(5017..5499)
                     /locus_tag="AC16HB_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5606910.1"
                     /translation="MTTPPPAAPVSERPSVEETPPSPAPAPARIVAPPAEPAPAPVAA
                     LDPAQLRPTPTAPRLEPPPAARLEPSPEALPPVVLRPVSRRQPQFPVQALREGVTRGS
                     VLARLSVATDGSVSAVTIVSSNPPRLFDRAAQAALAEWRFEPIPRPTSAQIELSFRAE
                     "
BASE COUNT          891 a         2033 c         2021 g          795 t
ORIGIN      
        1 gccgcgctcg tcgtcgcgcg cggcgacgaa ctccttggcc acgaggttca tgccgtcgtg
       61 caggctggtg accatacata cgtcgcagcc acggtagtgc tcgcgcaccg agtcggaatc
      121 gtggtgctcg atcagcagtc gcaccggcgc gtaggcggca tcgccgtact tcgcgttgat
      181 gcgcgccgcg atcgcgcgca cctcggcctc gaagcgctgg tattcgtcga gcgaggagcg
      241 gctcggcgcc gcgatctgga cgaaggtgaa gcgcccgatc cattcgggat gctgctcgaa
      301 cagccgctcg accgcgcgca accgctcgct gatgcccttc gtgtagtcga gccggtccac
      361 gccgagaccg atccgatgct ccgggtcgag gccgaaacgg ctgcgcagcc ggcgcgcgca
      421 ttcgtccgcc ggcgactgcg cggcggcggg ccaggcgatc gagatcggat agctctcgac
      481 ctgcgtcaga tgcccgcccg cgtgcaccgt cgagctttcg tgttcgatgc gcgcctcgag
      541 gaaacggtcc accgtctgca ggaagttgtt gcagtggtag cgcgtatgga agccgagaat
      601 cgtcgacccg agcagcccgc gcaggatctc ctcgcgccat gggcagatgc cgaacgactc
      661 cggattgggc cacgggatat gccagaaagt gatgatggtc gccttcggca gccgctcgcg
      721 gatcagcgcc ggcagcagcg cgaagtggta gtcctgcacc agcacgaccg ggtccgacgt
      781 ggtcgcctcg tccacgaccg cggcggcgaa gcgctcgttg acgtgctggt agtgcaccca
      841 gtcgtcgctg cggaacacgg ggcgcacatg cgcgatgtgg cacagtggcc acagcccttc
      901 gttggagaag ccgtagtagt agccctgctc ctcttcgcgc gtgagccaga tgcggcgcag
      961 cgtgtaggca ggctcgttcg gcggcacgcg caggtggccg cgtcggtcgg cggtctggcg
     1021 gtcggccgag ccgctgccgt gcgcgatcca cgtgcccgaa cacgcgcgca tcaccggctc
     1081 gaccgcagtg accagcccgc tggccggccg ctgcacctgg acaccgttcg cgacacgtac
     1141 gtggatgtac ggctcgcggt tggatacgac gatcacctcg tcgcccgaca actgttcgcg
     1201 cagcagcgcg cgcaacgagt cgggcgtcca ctgcagcgtc atgtcgtcgt gcagttgctg
     1261 ctccatctgg atctcgcgca gcagcgcgcg caggtcgccg accagaggct gcagttccgg
     1321 gcgtggatgc gagaacggcc ggaacacgcc ctcgccgcgc agcatcgcgc gcacaccggc
     1381 catccagccg cgccacgaca gcagcgcgac cgccaccgaa atcagcccga ccgccacgcc
     1441 gagcgcggtg aacgtcagca gcacgtactt gcgcgtgtcc tcgctgcggc gggtgacgaa
     1501 gctcatgtcg tgcacgatca gcagcgcccc gacggtggtt tcgccgtcgc gcaactcgct
     1561 caccgccaga tgcaccgggc cctgcggcag gttcagcacc tcgctgccgc cggccgacag
     1621 ccgcccgacc gcggagcagt cgatcgccgc cggcagcgtg cgcgtgtgcc gcagcatcct
     1681 gccttccggc gagcacaccg cgagcgcaaa gagccgctcg tcgctggtgg cgcgatcgag
     1741 cagcgcgtcg acgcgccccc agttgcgcgc ctgcacttgt tcgatcagca catcgccgag
     1801 cgtgcggccg atcaggttgg agcggatgtc gaggtcgcgc acgaaccagc gcagcgtcag
     1861 cgtgtcgacc agcggcaccg cgaagtacgc gagccccgcc attgcgatga acagcggcac
     1921 gatgaagcgc agtgagaagc ggacgggtcg gagcatcgga attgcgtcgg caggcttgtc
     1981 gctgcggcga cgacttcggc gtggcggccg gtggaatcta cgtacgccga cgggggcgca
     2041 atcgagccaa gttcacctta acacgcgatg cggtcgaggc cgacgcgcgc gctagcgaga
     2101 atgcgtgacg tacttacgcg aacctagcgg ccccggttcg atcgatcgcg cgagtcgagc
     2161 gtgcagatcc gcctcagaaa gcatcgtccc agcgcgccga cagccgcatt gcgcacatca
     2221 cgatgccgac catgctgtag gtctgaggga agttgcccca caactcgccg ctggcgacgt
     2281 cgatatcctc cgacagcagg ccgaaacgat tgcggcacgc gagcagccgc tcgaactcgt
     2341 cgcgcgcgcg agtgtgctcg ccgagcagcg cgagcgcctg gatccaccag aacgtgcaga
     2401 tcaggaacgc gctctccggc gcgccgaagt cgtcgggccg gtcgtagcgc agcaggaagt
     2461 tgccgcggcg cagttgccgc tcgaccgcct cgacggtgcc gcgcaggcgc ggatcgcccg
     2521 gtgccaggta gccgagttcg cccatcagca gcaggctggc gtcgagcgca tcgccgccga
     2581 actcggacac atacgtacgg cggttcgcgt cccatgcctg ggcgtcgatc gcggcgcgaa
     2641 tctccgtggc gcgcgcgcgc cagcccgtcg acgacgacga ttcgccgctt gccgtcgcga
     2701 tgcgcgcgag ccggtcgcat gcggcccaac acatcagtgc ggagaacgta tgcaccgact
     2761 gccggccgcg gtactcccag atgcccgcat cgggcgtcgc gtagcacgca aaggcgcgct
     2821 cgccgagccg ctcgagctgg cgcagcagcg cggcgtcgcc gcgccgatcc agccgctggt
     2881 cgaagaacgc atgcgacacg ccgaggatcg ccgcgccgta cacgtcgttc tgccgctgct
     2941 ggaacgcaag attgccgagg cgcaccggcc ccatgccgcg atagccggcc agcgccggca
     3001 tctcggactc ggccagctcg gcggcgccgg ccagcgaata caccggttgc agctgcgcgt
     3061 cggcggcatc ggcgatcacg ttctcgatgt agtgcaggta gcgttccagc gatcccgtcg
     3121 cgccgagccg gttcagcgcg ttcagcacga agtaagcatc gcgcagccag cagtagcggt
     3181 agtcccagtt gcgcgcggtg tgcggcgcct cggggatcga ggtcgtcagc gcggcgatca
     3241 cgccgccggt gtcgtcgaac gtgttcagct tcagcgtgat ggcagcgcgg atcaccgcgt
     3301 cctgccattc gaacggcagc gccaggttgc gcacccagcg ccgccagtag cgctgcgtct
     3361 cctccagcag atggcggccg acctcatgcg gattgccctg cagcgtttcg tcgggtccga
     3421 gcacgaaggt gagcgtgtcg tcgaccagca gcggccggcc ctcgatcagg ctggtgatcg
     3481 gcgcatccgt cgtcagccgc agcgggtacg ccggcacctg cgcgcgaatg tgatggctgc
     3541 cgaacgcgat cggcgcggcg ctggcgccgt agtcgcagcg cggcgcgaag cgcacccgca
     3601 cccgcggccg cccggcaacc cgccgcacga tgcgcacgat cgagatcggc gcgaacgtgc
     3661 gctcgtgctg atagaagcgc ggcgcgaagt cgatcagctc gaccgcgccg ccgttcgcat
     3721 cgcgcagcac gctgcgcagg atcggcgtgt tgcgctcgta ggcctgctcg atcgacgcgc
     3781 cgtgatcgag ctcgatgtcg aagcggccga gcgcggagtc gctgtccagc agcgcgcaga
     3841 acaccggatc gccgtcgaag cgcggcacgc acatccacac gatgctcgcg cgcgtgtcga
     3901 tcagcgcgcc gatgcgcgag ttgccgatcg ccgccagctc gagtcctcgg tccgccatcg
     3961 tcagtccacc agcacgccgg gggcgccgat tcgaccgcgt gcgatgtccg cgagcccgtg
     4021 cagcgcgagg tcgcccgcgt cgacgcgcca gcacggtact tcctcgagca gcgcgcggaa
     4081 gcgtcccttg tcggcaaagc cttcgcggaa cgccgcgcgc tcgaacgccg ggcccagccc
     4141 ggccagcacg ccgccggcga gatacacgcc gccgctggcg cgcagcgcca ggcacacgtc
     4201 accggcgaag ctgccgagcg cgcgcgagaa ccagcgcgcc gctgccagcg cgtgtgcatc
     4261 gcccgcctgc gcgcgctgtg cgacgcgcgc cggcgcatcg ggctcgcggc ggccgtgcat
     4321 gaacgcgtcg agccaagcga gcccgctgcc gcacagcagc cgttcccacg acacccgccc
     4381 ccagcgctgt tgcgcggcgt gcagcacgcg cagcgattcg tcgtcgatcg cggcaaacga
     4441 catgtggctc gcctcgcttt cgatgatgcg cgcgggcacg ccgccgccga gcacgcccgc
     4501 cacgccgagt ccggtgcccg gcccgagcac gacgcgcatc ggcccctgcg caacgccctg
     4561 ctgcaccatg acgaagccgg cgtcgagacc gaccgcgccg gccgcggcag cgacgaagtc
     4621 attgacgatc acgagccgtt cgaacccgag ctgcgccgcc agtttcggcg ccgcgaccga
     4681 ccaatcgcga ttggtgaaag tcagatcgtc gccggtgacc ggcatggcga gcgcaagcag
     4741 cgcgcgcttg accggccgtt gcacttcggc gaggtacgcg gcaagcagtt cgtgcagtcc
     4801 accgaaggcg gcgttttcca gctggcgctg cgtcgtgcgg cccgcggacg cgtgccagca
     4861 ggcgaagcgc gcattggtgc cgccgatgtc ggcgagcacc cagtcagaag catcgtcgag
     4921 cgccgggagc aggctgcaat acattgggca agtctgccat gcgcgcggcg cggcgccatt
     4981 ggcgcgattg cggatggccg tggccgcggc ttgcgcctac tcggcgcgaa acgacagttc
     5041 gatctgcgcg gaagtcggac gcgggatcgg ctcgaagcgc cactcggcga gtgcagcctg
     5101 cgcggcccga tcgaacaggc gcggcgggtt cgacgacacg atcgtcaccg cgctgacgct
     5161 gccgtcggtg gccacggaca ggcgcgccag caccgagccg cgcgtcacgc cttcgcgcag
     5221 cgcctgcacc gggaactgcg gctgccgtcg cgagaccggc cgcaacacga cgggcggcag
     5281 cgcctccggc gacggttcca gccgcgccgc cggcggtggc tcgaggcgcg gcgcggtcgg
     5341 tgtcggacgg agttgcgcgg gatcgagcgc cgccactggc gccggtgccg gttcggccgg
     5401 cggcgcgacg atgcgcgcgg gagccggcgc aggcgacggt ggggtttcct caacgctcgg
     5461 ccgttcggat accggcgctg cgggcggcgg cgtggtcatg cgcggctcgc tgtgcggctc
     5521 attcgtcgcg gcgcgcgaag tgctccgcgt ctcgcgcagc ggccgctcga tcgtgggcgc
     5581 gggcgcagcc gaccgatccg tgcgcggagc gatcggcggc gcatctgaaa ggaccggtgc
     5641 ggcgcgcact gcgcgttccg tcgacgatgc ggcggccggt gccgctgcag gcgacggcgc
     5701 agccatgacg gttggattcg ctgcgccgtg aaccgacgcg
//
LOCUS       DBNHBY010000354         1639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1374817, whole genome shotgun sequence.
ACCESSION   DBNHBY010000354 DBNHBY010000000
VERSION     DBNHBY010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1374817"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="AC16HB_08700"
     CDS             complement(<1..340)
                     /locus_tag="AC16HB_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606911.1"
                     /translation="MTRRDWRAGELRLLAAALVIAVAAVTSVGFFVDRIRLGLERDAK
                     QLLGADLVLSSDRPIADAERARARGAGLQTAETIVFPSMAVSERDANDTALAAVKAVS
                     TGYPLRGALRV"
     gene            complement(376..1080)
                     /locus_tag="AC16HB_08705"
     CDS             complement(376..1080)
                     /locus_tag="AC16HB_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5606912.1"
                     /translation="MEFKDRTVIVTGAAGNLGRAVAAAFAARGANLVLIDLRRETLQQ
                     AFGAEDARRMFVPVNLLDQAQVDAAVARAAERFGRIDALCNIAGGFRMGPAVHETSDA
                     DWGFLFDLNARTVLHMARAVVPRMLASGGGRIVNIGAFSAQKGAARMAAYTASKSVVI
                     RLTEAMSAELRDRNINVNCVLPTIIDTPENRAAMPDADPSRWVAPADLAHVIVFLASD
                     AVRAIHGAALPVTGLS"
     gene            1285..>1639
                     /locus_tag="AC16HB_08710"
     CDS             1285..>1639
                     /locus_tag="AC16HB_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5606913.1"
                     /translation="MSEHIGLIGLGAMGLGMAQSLRRRGYHVHVSDVRAEAAQAFASG
                     GGTAHATPAAVAAQCQVLISVVVNAAQTESVLFGEDGAASSLQPGSVFVMCSTVDPNW
                     SIALEKRLAGLGLLYL"
BASE COUNT          243 a          587 c          564 g          245 t
ORIGIN      
        1 tcacgcgcag tgcgccgcgc agcggatacc cagtcgacac cgccttgaca gccgcgagcg
       61 cggtgtcgtt cgcatcgcgt tcgctcaccg ccatgctcgg aaacacgatc gtctctgccg
      121 tctgcaatcc ggcgcctcgt gcgcgcgcgc gttcggcgtc ggcgatcgga cggtcggagc
      181 tgagcacgag gtcggcgccg agcagttgtt tcgcgtcgcg ctccagtccg aggcggatgc
      241 ggtcgacgaa gaagcccacg ctggtcaccg ccgccaccgc gatcaccagc gccgccgcca
      301 gcagccgcaa ctcgcccgcg cgccagtcgc ggcgggtcat gcgccacgcc tggcgcaccc
      361 gatcgaccgc ccccatcagc tcaaccccgt caccggcagc gcggcgccgt ggatcgcgcg
      421 cacggcgtcc gacgcgagga acacgatcac gtgcgcgaga tcggccggcg cgacccagcg
      481 cgaaggatcg gcgtccggca tcgcagcgcg gttctccggc gtatcgatga tcgtcggcag
      541 cacgcagttg acgttgatgt tcctgtcgcg cagctcggcg gacatcgcct ccgtgagacg
      601 gatcaccacg ctcttggacg cggtgtacgc ggccatgcgc gcggcgccct tctgggcgct
      661 gaacgcgccg atgttgacga tgcggccgcc gccgctcgcg agcatgcggg gcacgacggc
      721 gcgcgccatg tgcagcacgg tgcgggcatt gaggtcgaag aggaagcccc agtccgcatc
      781 cgatgtctcg tgcacggccg ggcccatgcg gaagccgccg gcgatattgc acagtgcgtc
      841 gatgcggccg aaacgctccg ccgcgcgcgc caccgccgcg tcgacctgcg cctggtcgag
      901 caggttgacc gggacgaaca tgcggcgcgc atcctcggca ccgaatgcct gctgcagagt
      961 ttcacgccgc agatcgatca gcacgaggtt cgcgccgcgc gcggcaaacg ccgccgcgac
     1021 ggcgcggccc aggttgccgg cggcgccggt gacgatcacg gtgcggtcct tgaattccat
     1081 gccatgtcct gaatcgctcg gcgtcatgat atcggtcacg cgggagcgcg ctgcggcggc
     1141 agcaccgtgt cccgaacgag tcagaccgct tgaccggtcc aaagtgcacc accacactgc
     1201 ccgcccgtcg ccgggcgcgt tcacacgata cagccggtgc cggcgggtcc gggatggcgt
     1261 cctgcgttgc ttttggagga gcgcatgagt gaacacattg gcctgatcgg cctgggcgcc
     1321 atgggcctag gcatggcgca gtcgctgcgg cgccggggct accacgtgca cgtgtccgac
     1381 gtccgcgccg aggcggcgca ggccttcgca tccggcggcg gcaccgcgca cgcgaccccc
     1441 gccgccgtcg ccgcgcaatg ccaggtgctg atcagcgtag tggtcaacgc cgcgcagacg
     1501 gagagcgtgc tcttcggcga ggacggtgcc gcatcctcgt tgcagccggg cagcgtcttc
     1561 gtgatgtgct ccacggtcga tccgaattgg tccatcgcgc tcgaaaagcg tctcgccggg
     1621 ctgggtctgc tctatctcg
//
LOCUS       DBNHBY010000355         6452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1374827, whole genome shotgun sequence.
ACCESSION   DBNHBY010000355 DBNHBY010000000
VERSION     DBNHBY010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1374827"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..692)
                     /locus_tag="AC16HB_08715"
                     /pseudo
     CDS             complement(69..692)
                     /locus_tag="AC16HB_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460241.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
     gene            complement(698..1282)
                     /locus_tag="AC16HB_08720"
     CDS             complement(698..1282)
                     /locus_tag="AC16HB_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR5606914.1"
                     /translation="MTTEQRVESVAPTARRPLHPMILIAALAVTAVSLIAAAQFLWPR
                     DAKSELAAQQPAAEAKTTSSKPATAKPAAAAPTAPKVATTACADCGVVVAVRESKQAG
                     QGTGLGAVAGAVVGGVVGNQMGGGRGKDAMTAIGVVGGGIAGHQIEKQARAQVVYHVD
                     VRMDDGTTRSFTQSSPLAVGAKVRVSGNHLALRG"
     gene            complement(1387..2919)
                     /locus_tag="AC16HB_08725"
     CDS             complement(1387..2919)
                     /locus_tag="AC16HB_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR5606915.1"
                     /translation="MSLAVVRSRGLSGMFAPEVTVEVHLANGLPSFTLVGLPDTEVKE
                     ARERVRAALQNCRFEFPLRRITVNLAPADLPKESGRFDLAIALGILAASGQIPARALD
                     RYEYAGELSLSGELRPVRGTLAMTYAVSKTPNLQGKPRGFIVPEDNADEAALVDGLDV
                     YPASHLLQVVAHLSADDTDDTEHQIRRHVAAVRSTPPAYPDFCEVKGQKGAKRALEIA
                     AAGGHSVLMVGPPGTGKTMLAQRFAGIVPPMTTDEALESAAVLSLVGQFAPQRWATRI
                     FRAPHHTASAIALVGGGNPPLPGEISLAHHGVLFLDELPEFDRRVLEVLREPLESGRI
                     TISRAARQVEYPAKFQLIGAMNPCPCGYFGHKTIACRCTPDQIARYQDRISGPLLDRI
                     DLRVEVSSLGEDELLQLSDGEPTRLTAARVAKAYDRALARQGKPNDRLISTEIERTCH
                     PEGAVRKLLHAATARLGWSARAYHRVLKVARTIADLDGTDELAAAHVAEAIQYRRALR
                     EN"
     gene            3171..4028
                     /locus_tag="AC16HB_08730"
     CDS             3171..4028
                     /locus_tag="AC16HB_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606916.1"
                     /translation="MGYLLLYGALLVICGVVFLWTWQKLRAQREAAAAREASILAAVQ
                     AAGAVAPTGDGETNVDAGLSTNLRGQRGIEVAEESIDIEALLSSVSSGAAVRARAQLD
                     EPTNIQPMLSGDWPSTGMQTGGPSLRPAPNSAVNTTPPAVPRPPAGMDIDVPLRNLVL
                     AWFEARGYRPAPASTALRPIDVVLRHREDPTRNYAFMYDPGRATGPRASSLLDLARSA
                     GLSKLAIAAEHGGDSALRANRVKNVRMIDWPTLEADLKKLDREIAERIVGIARQRSAP
                     IERVVATAR"
     gene            4118..4741
                     /locus_tag="AC16HB_08735"
     CDS             4118..4741
                     /locus_tag="AC16HB_08735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5606917.1"
                     /translation="MFSDRDDAARQLALALAKYKGTKPLVLGIPRGAVPMAKLIAAQL
                     GGEVDVVLVRKIGAPGHAEYAIGAIDESGWSYLNPDAAWVQASHEYLEREKQAQLATM
                     RERRRRYTEHRTAIDPAQRTVIVVDDGLATGSTMIAALHALREKKPARLVCAVPVSPP
                     ETLERVRPLADEVVCLYAPAAFYAVGQFYRQFDQVEDDEVVAALSGK"
     gene            complement(4696..5370)
                     /locus_tag="AC16HB_08740"
     CDS             complement(4696..5370)
                     /locus_tag="AC16HB_08740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5606918.1"
                     /translation="MNPPSRAVRIAQRDIALPGDLTWPATANALVLFSHGSGSSRLSP
                     RNRYVAEVLNEAGLATLLFDLLTPGEDTDRAARFDIPLLAERLLGAAHWCREQHDLAR
                     LPLGLFGASTGAAAALIAAARLGPDVRAVVSRGGRPDLAPPTDIERVAAPTLLIVGEY
                     DREVLLLNRRAAKSLRAPHAERIVPGATHLFEEPGTLEQAAAAARDWFVRYLPDKAAT
                     TSSSST"
     gene            complement(5367..6440)
                     /gene="amrS"
                     /locus_tag="AC16HB_08745"
     CDS             complement(5367..6440)
                     /gene="amrS"
                     /locus_tag="AC16HB_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR5606919.1"
                     /translation="MSYPARWWHRLADGRIQCDLCPRDCRLHEGQRGLCFVRQRTGDA
                     MVLTTYGRSSGFCIDPIEKKPLNHFYPGSSVFSFGTAGCNLACKFCQNWDISKSAEMD
                     RLMDRAAPSEIAQAAQRTGAASVAFTYNDPVIFAEYAMDAADACHARGVKTVAVTAGY
                     MHAEPRREFYAKMDAANVDLKAFTEDFYFKLTGAHLAPVLDTLKYLVHETHVWTEITT
                     LLIPGRNDSSDEIRALSAWVKKELGTDVPLHFTAFHPDWKMTDVPATPPSTLSRARRI
                     AQEEGLHYVYTGNVHDTEGGTTFCPQCHAALIERDWHRILRYDLAEDGRCPHCATAIA
                     GRFGKFEHAFGARRIPVRLHRAP"
BASE COUNT          986 a         2205 c         2284 g          977 t
ORIGIN      
        1 ccgcagcttc gcggaactga tgcagtcgat cgaccgcgcc gcggcgcggc tcgttccgac
       61 taagtgagct acgcggcgcg ccggaatacc agcgtcaggt tgttggccgg cattgcgacg
      121 cgctcgcgca gcgcgagccg catcttctgc gcttcggcaa cgacggcggc gagccggcgc
      181 aggccccagg caggattgcg cgcccgcaga tcagcgtcga acgcgaggtt gctcggcgca
      241 gtcgactcgc cgtcgaccaa gaacggtccg tagatcacca gcacgccatc cgccgcgaga
      301 tggcccgcag ccccgcgcat caaccccgcg cacgcggccc aaggtgcgat gtgcagcagg
      361 ttggcgcaga agatcgcgtc gaccagcggc gcgccgggcc attccgacgc cagcacatcg
      421 agccgtcgcg gcggcaatag attggcaagg tgcgtttccg cgcgccacgc ggcgatcgag
      481 gcgagcgcgt ccgcatcggc atcggtcgcg agccatgtct accgcggcag gtgcgcggcg
      541 aagtacgccg cgtgctggcc agtgccggcg ccaatttcga gcgccgtgcc gcacgccggc
      601 agcacgcgtt ggagtacgtc gaggatcggc cgctgattgc gctcggcggc ggcgctgtac
      661 tggcgcgcgg cgccgggatg gcagtcggtc aagtcgttca gccgcgcagc gcgaggtggt
      721 tgccgctaac acgcaccttg gcgccgaccg cgagcggcga cgattgggtg aacgatcgcg
      781 tcgtgccgtc gtccatcctt acgtcgacgt gatacacgac ttgcgcgcgc gcctgtttct
      841 ctatctggtg gccggcgatg ccgccgccga ccacgccgat cgccgtcatc gcatccttgc
      901 cacggccgcc gcccatctgg ttgccgacca cgccgccgac caccgcgccg gcgaccgcgc
      961 ccaggcccgt tccctggccg gcctgcttcg attcgcgcac ggcgaccacg acgccgcagt
     1021 ccgcacacgc ggtggtggcg actttcggtg cggtgggcgc ggccgcggcg ggtttggccg
     1081 tggctggctt gctggaggtg gtcttcgctt ccgcagctgg ctgttgcgcg gccaactccg
     1141 acttcgcgtc ccgcggccac aggaactgcg cagccgcgat cagcgacacc gccgtgaccg
     1201 ccagcgcagc gatcaggatc atcggatgca acggacgccg tgcggtcgga gcgacgcttt
     1261 caactcgttg ttcggtggtc atgagtcctc ctgcgaaatc ccgaatccca attcaacgac
     1321 gaattgcggg ccccgggatc acgcgaccgg gttacgacat ccgcatcaat gacgcgggcg
     1381 gggtcttcag ttctcgcgca gcgcgcgccg gtactggatc gcctcggcca cgtgtgcggc
     1441 ggcgagttcg tcggtgccgt ccaggtcggc gatcgtgcgc gccaccttca gcacgcggtg
     1501 ataagcgcgc gccgaccagc cgagccgcgc cgtggccgcg tgcaacagct ttcgcaccgc
     1561 gccctcggga tggcaggtgc gctcgatctc ggtcgagatc agccgatcgt tcggcttgcc
     1621 ctgccgcgcg agcgcgcggt cgtacgcctt ggccacgcgc gccgcggtca gccgcgtcgg
     1681 ttcgccgtcg gagagctgca gcagctcgtc ttcgccgagc gacgacacct cgacgcgcag
     1741 gtcgatgcgg tcgagcagcg gtccgctgat gcggtcctga tacctggcaa tctgatccgg
     1801 cgtgcagcga cacgcgatcg tcttgtggcc gaagtagccg cacgggcatg gattcatcgc
     1861 gccgatcaac tggaacttcg ccgggtactc gacctggcgc gcggcgcgcg agatcgtgat
     1921 gcggccggac tcgagcggct cgcgcagcac ttcgagcacg cggcgatcga actccggcag
     1981 ctcgtcgagg aacagcacgc catggtgcgc gagcgagatc tcgcccggca gcggcggatt
     2041 gccgccgccg accagcgcga tcgcgctcgc ggtgtgatgc ggcgcgcgga agatgcgcgt
     2101 ggcccagcgc tgtggcgcga actgaccgac cagcgacagc accgcggcgg actccagcgc
     2161 ctcgtcggtc gtcatcggcg gcacgatgcc ggcgaagcgc tgcgccagca tcgtcttgcc
     2221 ggtaccgggc gggccgacca tcagcacgct gtggccgccg gcggccgcga tttcgagcgc
     2281 gcgcttggcg cctttctgtc ccttgacctc gcagaaatcg ggatacgccg gcggcgtcga
     2341 ccgcaccgcg gcgacgtgcc gtcggatctg atgctcggtg tcgtcggtgt cgtcggcgga
     2401 caggtgcgcg accacctgca acaggtgact tgccggatag acatcgagcc cgtcgacgag
     2461 cgcggcttcg tcggcgttgt cctccggcac gatgaagccg cgcggcttgc cctgcagatt
     2521 cggcgtcttc gacaccgcat acgtcatcgc cagcgtgccg cgcaccggcc tcagttcgcc
     2581 ggacagcgag agttcgcctg cgtactcgta gcgatcgagc gcgcgcgcgg gaatctggcc
     2641 actggcggcg aggatgccga gtgcgatcgc cagatcgaaa cggccggact ccttcggcaa
     2701 gtcggctggc gcaagattga ccgtgatccg ccgcagcggg aattcaaagc ggcagttctg
     2761 cagtgcggcg cgcacgcgct cgcgcgcctc cttgacctcg gtgtcgggca ggccgaccag
     2821 cgtgaacgac ggcaggccgt tggccagatg gacctcgacc gtcacctccg gggcgaacat
     2881 gcccgacagg ccgcgactgc ggacgacggc gagactcatt caggtcggtc cgggaatggc
     2941 gcgcgattgt acgcacggcg ctccgcgccg ctgccggggt acggcatgcg aatgcgaacg
     3001 gccgatgcgc gcgtgcgtgg cgcgcatgcg ggtgaatgcg ttcggccgga cggcggatcg
     3061 tggcccgcgg aacccgtacc gagaatcgca gatagtgctt gccacgctgt ggcgcacagc
     3121 caagatcaac gactgcacga aaggcacgcg gcgacgcgtc tggtttccgg atgggctatc
     3181 tgctgctgta cggcgcgctg ttggtcatct gcggggtcgt gttcctctgg acctggcaga
     3241 aactgcgcgc gcagcgtgaa gccgcggcgg cacgcgaagc ttcgatcctc gcagcggtgc
     3301 aggccgccgg cgcagtggcg ccgaccggag acggcgaaac caacgtcgac gccgggctgt
     3361 cgaccaacct gcgcggccag cgcggcatcg aggtcgccga ggaaagcatc gatatcgagg
     3421 cgctgttgtc gagcgtgtcg tcgggtgcag ccgtgcgggc gcgcgcacaa ctcgacgagc
     3481 ccaccaacat ccaaccgatg ctatccggtg actggccgtc gacgggcatg cagaccgggg
     3541 gcccgagcct gcggccggcg ccgaactccg cagtgaacac cacgccgccg gcggtgccgc
     3601 ggccgcccgc cggcatggat atcgacgtgc cgctgcgcaa tctggtgctg gcgtggttcg
     3661 aagcgcgcgg gtaccggccc gcgccggcat cgaccgcgct gcgcccgatc gacgtcgtcc
     3721 tgcgccaccg ggaagacccg acgcgcaact acgcattcat gtacgacccc ggccgcgcga
     3781 ccgggccgcg cgcgagcagc ctgctcgatc tcgcccgatc ggccggactg tcgaagcttg
     3841 caatcgccgc cgagcacggc ggcgactcgg cgctgcgcgc caaccgtgtg aagaacgtgc
     3901 gcatgatcga ctggccgacg ctcgaagcgg acctgaagaa gctcgaccgc gaaatcgccg
     3961 aacgcatcgt cggcatcgcg cgccaacgct cggcaccgat cgagcgtgtc gtcgcgaccg
     4021 cacgctgagc ggcaaggctc ggctccccat caacctgcgc tgcggcaaca cgcgagacgt
     4081 gcgcgggcgt agtcttgcgc aggtccgggg tgattcgatg ttctccgatc gtgacgacgc
     4141 ggcacgtcag ctcgcgctgg cgctggcgaa gtacaaggga acgaaacccc tcgtgctcgg
     4201 catcccgcgc ggtgcggtgc cgatggcgaa gctgatcgcc gcgcagttgg gcggcgaggt
     4261 cgacgtggtg ctggtgcgca agatcggcgc gccgggacac gcggagtacg cgatcggcgc
     4321 gatcgacgag tcgggctggt cgtacctgaa tccggacgcg gcctgggtgc aggcttcgca
     4381 cgagtacctc gagcgcgaaa agcaggcgca gctggcgacg atgcgcgagc ggcgccgtcg
     4441 ctataccgag caccgcacgg cgatcgatcc ggcgcagcga acggtgatcg tcgtcgacga
     4501 cgggttggcg accggatcga cgatgattgc ggcactgcac gcgctgcgcg agaagaagcc
     4561 ggcgcggctc gtgtgcgcgg tgcccgtgtc gccgccggaa acgctggagc gcgtgcggcc
     4621 gctcgccgac gaagtggtgt gcctgtacgc accggccgcg ttctacgcgg tcgggcagtt
     4681 ctaccggcaa ttcgatcagg tcgaggacga cgaggtcgtc gctgccttgt ccggcaagta
     4741 acgcacgaac cagtcgcgcg ctgccgcggc cgcctgctcg agcgtgccgg gctcttcgaa
     4801 cagatgcgtg gcgcccggca cgatgcgctc ggcgtgtggc gcgcgcaggc tctttgcggc
     4861 gcggcggttg agcagcagca cctcgcggtc gtattcgccg acgatcagaa gcgtcggcgc
     4921 ggcgacgcgc tcgatgtccg tgggcggcgc caggtcgggc cgtccgccgc gcgaaacaac
     4981 cgcgcgcacg tcgggaccga ggcgggctgc ggcgatcagt gcagccgccg caccggtgct
     5041 ggcgccaaag aggccgagcg gcaggcgcgc aagatcgtgc tgctcgcggc accagtgcgc
     5101 cgcgccgagc aggcgctcgg ccagcagcgg aatgtcgaag cgcgctgcgc ggtccgtgtc
     5161 ctcgcccggc gtcagcaggt cgaacagcag cgtggccagg ccggcctcgt tcagaacctc
     5221 ggcgacgtag cgattgcgcg ggctcagccg gctgctgcca ctgccgtgcg agaagagcac
     5281 cagcgcgttc gctgttgccg gccacgtcag atcgcccggc agcgcgatgt cgcgctgcgc
     5341 gatccgcacg gcacgcgacg gcgggttcat ggcgcgcgat ggagccggac cggaatgcgg
     5401 cgcgcgccga acgcgtgctc gaactttccg aagcgtcccg cgatcgcggt cgcgcaatgg
     5461 gggcagcggc cgtcctcggc caggtcgtac cgcaggatgc ggtgccagtc gcgctcgatc
     5521 agcgcggcgt ggcactgcgg gcagaacgtc gtgccgccct cggtgtcatg cacgttgccg
     5581 gtgtagacgt agtgcaaccc ttcttcctgc gcgatacggc gcgcacgcga cagcgtcgac
     5641 ggcggcgtgg ccggcacgtc ggtcatcttc cagtcgggat gaaacgccgt gaagtgcagc
     5701 ggcacgtcgg tcccgagttc tttcttcacc catgccgaca gcgcgcggat ctcgtcggac
     5761 gagtcgtttc tgccgggaat cagcagcgtc gtgatctcgg tccacacgtg cgtctcgtgc
     5821 accaggtact tcagggtgtc cagcaccggc gccagatgcg cgcccgtgag cttgaagtag
     5881 aagtcctcgg tgaacgcctt caggtcgacg ttggcggcgt ccatcttcgc gtagaactcg
     5941 cggcgcggct cggcatgcat gtagccggcg gtgaccgcca ccgtcttcac gccgcgtgcg
     6001 tggcaggcgt ccgccgcatc catcgcgtac tcggcgaata tcaccgggtc gttgtaggtg
     6061 aacgccacgc ttgcggcccc ggtgcgctgt gcggcctgcg cgatctcgga cggcgctgcc
     6121 cggtccatga gccggtccat ctcggccgac ttggagatgt cccagttctg gcagaacttg
     6181 catgcgaggt tgcacccggc ggtgccaaac gagaacaccg acgagccggg atagaagtga
     6241 ttgagcggct tcttctcgat cgggtcgatg cagaacccgg acgaacgacc gtaggtcgtc
     6301 agcaccatcg cgtcgcccgt gcgctgccgc acgaagcaca gtccgcgctg gccttcgtgc
     6361 agcctgcagt cgcgcgggca caggtcgcac tggatgcggc cgtcggcaag ccggtgccac
     6421 cagcgcgccg gatagctcac ttcatcgcgc gc
//
LOCUS       DBNHBY010000356        14474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_426862, whole genome shotgun sequence.
ACCESSION   DBNHBY010000356 DBNHBY010000000
VERSION     DBNHBY010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14474
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_426862"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="AC16HB_08750"
     CDS             complement(<1..145)
                     /locus_tag="AC16HB_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HIR5606920.1"
                     /translation="MGVGHYENFPVASLLLPAALRPAVRAIYRFARAADDIADEGAAP
                     PQER"
     gene            244..330
                     /locus_tag="AC16HB_08755"
     tRNA            244..330
                     /locus_tag="AC16HB_08755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Leu,seq:tag)
     gene            380..1681
                     /gene="tig"
                     /locus_tag="AC16HB_08760"
     CDS             380..1681
                     /gene="tig"
                     /locus_tag="AC16HB_08760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR5606921.1"
                     /translation="MMQNVETLSALERRIDIAVPAAEINKEVTARLIKLSRTLRMPGF
                     RPGKVPMKMVASSYGAQVQAEVLNDKVGEAFNTALKDSNLRVAGAPQLTPKEGVGEQL
                     SFSATFEVYPEIRIGDLSNAEVTRAVCPVGEPEVDRTIEIMRRQRAKFEPVERGAADG
                     DRLTIDFKGTIDGTPFEGGSGTDFAFTLGQGRMLPDFDAALRGVAAGASKTFPVQFPA
                     DYRATDLAGKSAQFEVTVKKVEQPLLPPVDAEFARSLGIADGDIAKMRSEVKSNLDRE
                     VSTRLKARTKDSVMAALLGATQFEVPKSLVLEDQQRLAEMARADLVARGIDAKEAPLP
                     PEVFAPQAERRVRLGLLIAELVRGQNLQARQDQVRKAIEEIAQSYEKPAQVIQWYLGN
                     RERLAEVEAMTLENNVVEWVLQRAKVNDVAVPFDELMGHGV"
     gene            1686..2321
                     /gene="clpP"
                     /locus_tag="AC16HB_08765"
     CDS             1686..2321
                     /gene="clpP"
                     /locus_tag="AC16HB_08765"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR5606922.1"
                     /translation="MTQRMEHPPIDPQSLGMVPIVIEQSGRGERAYDIYSRLLRDRVV
                     FLVGPVNEQTANLVVAQLLFLESENPDKDIALYINSPGGSVYAGMAIYDTMQFIKPNV
                     STICVGMAASMGAFLLAAGAKGKRYALPNSRIMIHQPSGGSQGQASDIEIQAREILYL
                     RDRINRILAERTGQSVDKIRQDSDRDNFMSAEDAVSYGLIDRIFVKRGEAG"
     gene            2341..3603
                     /gene="clpX"
                     /locus_tag="AC16HB_08770"
     CDS             2341..3603
                     /gene="clpX"
                     /locus_tag="AC16HB_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR5606923.1"
                     /translation="MSDKKGSSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIDLCN
                     DIIRDESAGEPAAAAKSDLPTPQEICAILDQYVIGQEKAKRILSVAVYNHYKRLKHLS
                     TKDEVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVENI
                     IQKLLQNCNYEVEKAQKGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTI
                     ASVPPQGGRKHPNQDFVQIDTTNILFICGGAFDGLEKVIRNRSEKSGIGFGAEVTSPR
                     DRSVGDVLREAEPEDLIKFGLIPELVGRLPVVATLDELSEKALVEILVEPKNALIKQF
                     RRLLDMEGVELEVRPDALQAIAKKALKRRTGARGLRSIVEAVLLDVMYDLPGMQNVIK
                     VVIDDNAIAGGRPILIYSDQPKVAGAPR"
     gene            3845..6190
                     /gene="lon"
                     /locus_tag="AC16HB_08775"
     CDS             3845..6190
                     /gene="lon"
                     /locus_tag="AC16HB_08775"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5606924.1"
                     /translation="MVIPLFVGRPKSIKALETAMEAGKSIMLVAQKNAAKDEPSAEDI
                     YSIGCVANILQMLKLPDGTMKVLVEGAQRAQIHEIRDAQTHYTCDLTPMAPETSVSPE
                     LEALRRAIVAQFDQYVKLNKKIPAEILTSIAGIDEAGRLADTIAAHLPLKIEQKQKIL
                     EMSSLAERLEHLLAQLETEIDILQVEKRIRGRVKRQMEKSQREYYLNEQVKAIQKELG
                     EGEEGADLEDLDKKVKAARMPKQARQKAEGELKKLKLMSPMSAEATVVRNYIDTLVNL
                     PWKKKSKINNDLANAQKVLDEDHYGLDKVKERIVEYLAVQQRVDRLKAPILCLVGPPG
                     VGKTSLGQSIARATNRKFVRTALGGVRDEAEIRGHRRTYIGSMPGKILQNLSKVGVRN
                     PLYLLDEVDKMGQDFRGDPSSALLEVLDPEQNHTFVDHYVEVDYDLSDVMFVATANTM
                     NIPPPLLDRMEVIRLSGYTEDEKTNIALRYLLPKQMRNNGVKKEELEVSEGAVRDIIR
                     YYSREAGVRALERELSKICRKVVKNALLKESGGKVKVTPRNLDKFLGVRRYTFGMAEK
                     EAQVGQVTGLAWTEVGGELLTIEAAQMPGKGNIIRTGSLGDVMKESVEAARSVVRSRS
                     RRLGIKDEVFEKSDIHIHLPEGATPKDGPSAGIAMTTAFVSTLTGIPVRPDVAMTGEI
                     TLRGEVLAIGGLKEKLLAAHRGGIKTVLIPEENVKDLAEIPDNVKNRLEIIPVKWIDR
                     VLEIALQRQPAPLAEDEAAAAAAVAPAAPAPAAADGKFVKH"
     gene            6399..6671
                     /locus_tag="AC16HB_08780"
     CDS             6399..6671
                     /locus_tag="AC16HB_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5606925.1"
                     /translation="MNKTELIEHIAKQADISKAAAGRALEALVGGIRVALKKGNSVTL
                     VGFGTFMVGKRAARTGRNPRTGAAIKIKAAKVPKFRPGKDLKDAVN"
     gene            6718..6793
                     /locus_tag="AC16HB_08785"
     tRNA            6718..6793
                     /locus_tag="AC16HB_08785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6751..6753,aa:Val,seq:tac)
     gene            6838..6914
                     /locus_tag="AC16HB_08790"
     tRNA            6838..6914
                     /locus_tag="AC16HB_08790"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6872..6874,aa:Asp,seq:gtc)
     gene            6998..8932
                     /locus_tag="AC16HB_08795"
     CDS             6998..8932
                     /locus_tag="AC16HB_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR5606926.1"
                     /translation="MFDTVRSHRRWLMIFLAVLVFPSFVITGVYSWNRFVSAENAVAT
                     IGSEKISPQQFDAAHRERVEQLRQALGANFDPKVFDSAEARAAVLDNLLSERALAQEA
                     ARDGIVISEARLREVIASVPAFQQDGKFSYERYKTLLAAQGMNEFAFEQRVRDDLLRQ
                     TLLRAIGQSAFVPKAVLDRINRLGEEQREVRLLRFEPKDYATKVTVTDEAIDAYYKGN
                     RAQFEIPESVKAEYLVLTLDDVAGQIAVSEAEVKAYYEQNQARFGEPEQRRASHILLT
                     VGEGGTATDKAGARKKAEELLAKLRANPNSFAQLAKENSKDPGSAASGGDLGFFGRGM
                     MVKAFEDAAFKLKEGEISDIVESDFGFHIIRVTAIKPAQVKPFEQVRAEIEKDYRRQQ
                     AQKKFAEAVEQFTNTVYEQSDSLKPAADKLKLAIQTADRVTREGLPAKPGAPQIFTPR
                     VLQALFSDDGINKKRNTEAIEVAPNTFVSARVVEHRPATLRPLAEVRDSIKARVERQE
                     AARLAREAAQKALAGLRQSPSAAGFAPPVTISRAQPQGMSPSALTAIMRVPADKLPTF
                     IEAELDGGSAAIVQVLSAKMPDAPNVQQREAFARSLQQQAGTADDVAYLDALKAKFKV
                     EVLKPDLKPVATPQGEDKKN"
     gene            complement(8933..9988)
                     /gene="mnmH"
                     /locus_tag="AC16HB_08800"
     CDS             complement(8933..9988)
                     /gene="mnmH"
                     /locus_tag="AC16HB_08800"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR5606927.1"
                     /translation="MDRSVIACDEALRRLEEFDALIDVRSESEFAEDHLPGALNCAVL
                     DDAERALIGTLHKQDAPFIARRRGAALVARNIARHLETSFAGKPRDWKPLVYCWRGGQ
                     RSGAMTHVLARVGWHARQLEGGYRAYRRAVVQALVELPARLEFRVVCGTTGSGKSRLL
                     RELERAGAQVLDLENLAQHRGSVLGGLPRQPQPSQKQFESRIWWRLRGFDAERAVFVE
                     SESRKVGDLRVPDALIDRMRASHCLRLELSLPDRIALLRDEYLHLEQDRATLFRQLDC
                     LVPLHGRDRVQEWKRLAEHGDWAQLVERLLRDHYDPVYLKSIGRNFARVGDARLVSPA
                     SASEDDFARLAREIASG"
     gene            complement(9990..10628)
                     /locus_tag="AC16HB_08805"
     CDS             complement(9990..10628)
                     /locus_tag="AC16HB_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR5606928.1"
                     /translation="MAASRAIVSIAVLFCCVFGFGAPRAEVRPSVLVLGDSLSAEYGL
                     PRDTGWVKLLANRLATLATGRAKYTVVNASISGETTSGGRTRLPTLLKQHRPAIVVIE
                     LGANDGLRGLPLAALRENLSEMASLSRAGGARVLLIGMRIPPNYGRDYADRFAATFAE
                     VARAQKAALVPFLLDGFADRLDYFQADRIHPNERAQPLMLDNVWPRLRPLLK"
     gene            10661..11371
                     /locus_tag="AC16HB_08810"
     CDS             10661..11371
                     /locus_tag="AC16HB_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606929.1"
                     /translation="MQTGTDALLASGLTKIVQDATGSLTILDDINLKIAAGETVAIVG
                     ASGSGKSTLLGLLAGLDVPTRGGVLLFGEDLFARNEDDRARVRGAKIGFVFQSFQLLP
                     HLTALENVLLPLELAGRAPDGIDPVDAAKALLERVGLAERLAHYPKYLSGGEQQRVAL
                     ARAFVSRPPLLLADEPTGSLDAHTGEAVIELMFELNRERDSTLVLVTHDPDIAARCSR
                     RIELHAGRIRSDSASAAA"
     gene            complement(11357..12805)
                     /locus_tag="AC16HB_08815"
     CDS             complement(11357..12805)
                     /locus_tag="AC16HB_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR5606930.1"
                     /translation="MGIPLYTVAALRQIETAVQADLPPGELMRRAGTAAAQLIHERRH
                     DAHVLIVCGPGNNGGDGYVCATELRALGHRVTVVALAPPAADDARAAALRWADAGGSC
                     IFALPVSGEFDLIVDAMFGIGMARPLAGAFLDAARWMSARRGRVVAIDVPSGLDADRG
                     AWVGDVAGVQASETVTFLGAKPGLYTHDGADAAGRVIVDGLRIAPPASSGALLDPSDF
                     AAVLAPRARNTHKGTYGNALVVGGHVGMVGAALIAARAALRLGAGRVYVDCIGAPEMR
                     LDPVQPELMFRRGADVIPVDAIVIGCGLGADEGAKAALRFALLQPTPLVLDADALNLI
                     AGDEALRGALGNHRHPRVLTPHPLEAARLLGRGARDVQSDRIRAATDLAAALGSIVVL
                     KGAGTLVAQPDGRYGICPTGTPALATAGTGDALAGMIGALVAQRFAADVATCAATWLH
                     GAAADEFGDELGLVAGAIAPIAARILARLRRR"
     gene            complement(12817..14076)
                     /locus_tag="AC16HB_08820"
     CDS             complement(12817..14076)
                     /locus_tag="AC16HB_08820"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HIR5606931.1"
                     /translation="MHVAVIGAGIIGVTSAYYLRQQGFQVTVIERNPGVAQETSFANA
                     GVIAPGYIAPWAQPGMPSKVLRHLFAREAPVVFRPCFDFAQWRWLARWLAECKVERFR
                     RNKARMQRLAFYSRDCLHALRQRHGIEYEQTQGYLQLYRTEAELARTALARALLKEAG
                     VLHVLLSGADCRRLEPALVDGTPLAGGLHLPEDETGNCAFFAKRMKDISAEQGVQFRF
                     GTAVDALEVEAGRVARLRLRDGTLAADAVVVAAGVSSPRLVRPLGIRLPLYPIKGYSA
                     TAAITRPENAPMISVMDETYKVAVTRMGNRLRIAGTAEIGSRTLTLRDASLGTLLRVA
                     RDWFPGAAAYGQAQFWVGARPMLPDGPPLLGPTPIANLHLNVGHGSTGWAMACGSARV
                     VADLIANRKPDIDLEGLTLDRYARRSG"
     gene            complement(14095..>14474)
                     /locus_tag="AC16HB_08825"
     CDS             complement(14095..>14474)
                     /locus_tag="AC16HB_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5606932.1"
                     /translation="AEFIASLGLSPVWLIVALTLFYILLGCFLDGISMVVLTMGVLLP
                     TVTAAGIDLIWFGIFVVLVVEMAQITPPVGFNLFVLQGMTKREIGYIARVTLPFFLLM
                     VAAVVLIYIFPQLVTFLPSRMRL"
BASE COUNT         2682 a         4697 c         4940 g         2155 t
ORIGIN      
        1 gccgctcttg aggcggcgcg gcgccttcgt ccgcgatgtc gtcggcggcg cgcgcaaagc
       61 ggtagatcgc gcgcaccgcg ggacgcagcg ccgcgggcag caggagcgat gcgacgggaa
      121 agttctcgta gtggccgacg cccatgcggt tcacgctagc acgcgcgccg ggcgcgcaag
      181 gcgatccatt agaatgcgcg ccgaatcggc gagcggcaca ggacgtccgc cgccgaggtc
      241 gtcgcgaagg tggcgaaatt ggtagacgca ccaggtttag gtcctgacgc cttcaccggc
      301 gtgggggttc gagtcccccc cttcgcacca gtgactcgtt agatcccgcg cgtcggacgc
      361 gggcacgcaa ctcgatcgga tcatgcagaa cgttgaaacg ctgagcgcgc tggagcggcg
      421 catcgatatc gccgtccccg cggccgagat caacaaggaa gtcacggcgc gcttgatcaa
      481 gttgtcgcgc acgctgcgga tgccgggttt tcgacccggc aaggtgccga tgaagatggt
      541 cgcgtcatcg tacggtgcac aggtgcaggc cgaggtgctg aacgacaagg tcggcgaggc
      601 gttcaatacc gcgctgaagg acagcaacct gcgcgtggct ggcgcgccgc agttgacgcc
      661 gaaggaaggc gtgggcgagc agctttcgtt ttcggccacg ttcgaggtgt atcccgagat
      721 caggatcggc gacctgtcca acgccgaagt cacgcgcgcg gtgtgcccgg tcggcgaacc
      781 agaggtcgac aggacgatcg agatcatgcg gcgccagcgc gcgaagttcg aaccggtcga
      841 gcgcggcgcc gccgatggag atcggctgac gatcgatttc aagggaacga tcgacggcac
      901 gccgttcgag ggcggcagcg gtaccgactt cgcgttcacg ctcggccagg ggcggatgct
      961 gcccgacttc gacgctgcgc tgcgcggcgt ggccgctggc gccagcaaga cgtttccggt
     1021 gcagtttccc gccgactatc gtgcgacgga tctggccggc aagagcgcgc aattcgaggt
     1081 gaccgtcaag aaggtcgagc agcctctgct gccgccggtc gacgcggagt tcgcgcgctc
     1141 gctcggcatc gccgacggcg acatcgccaa gatgcgctcc gaggtgaagt cgaacctcga
     1201 tcgggaagtg tcgacgcggc tgaaggcgcg caccaaggac agcgtgatgg ccgcgctgct
     1261 gggcgccacg caattcgagg tcccgaagtc gctggtgctg gaagatcagc aacggcttgc
     1321 cgagatggcg cgcgcggacc tcgtcgcgcg cggcatcgac gccaaggagg cgccgctgcc
     1381 gccggaggtg ttcgcgcctc aggccgagcg gcgcgtccgc ctgggcctgc tgatcgcgga
     1441 actggtccgg gggcagaacc tgcaggcgcg ccaggaccag gtgcgcaagg cgatcgagga
     1501 gattgcgcag agctacgaga agccggcgca ggtgatccag tggtatctcg gcaaccgcga
     1561 gcggttggcc gaggtcgaag cgatgacgct ggagaacaat gtggtcgaat gggtgctgca
     1621 gcgcgcgaag gtcaatgacg tggcggtgcc gttcgacgaa ctgatgggcc acggagtctg
     1681 aacagatgac gcagcgaatg gagcatccac cgatcgatcc ccagagcctg ggcatggtgc
     1741 cgatcgtcat cgaacagagc gggcgcggcg agcgcgccta tgacatctat tcgcgcctgc
     1801 tgcgcgatcg cgtggtgttt ctggttggac cggtgaacga gcagaccgcc aacctggtgg
     1861 tcgcacagct gctgttcctg gagtccgaga atccggacaa ggacatcgcg ctctatatca
     1921 actccccggg cggatcggtg tacgccggca tggcgatcta cgacacgatg cagttcatca
     1981 agcccaacgt gtcgacgatc tgcgtgggca tggcggccag catgggcgcg tttctgctcg
     2041 ccgcgggcgc caagggcaag cgctacgcgc tgccgaactc gcgcatcatg attcaccagc
     2101 cgtcgggcgg atcgcagggc caggcgtccg acatcgagat ccaggcccgc gagatcctgt
     2161 acctgcgcga tcgcatcaac aggatcctgg ccgagcgcac cggacagagc gtcgacaaga
     2221 tccgacagga cagcgaccgc gacaacttca tgtccgcgga ggatgcagta agctacgggc
     2281 tgatcgacag gatcttcgtc aagcggggcg aggccggctg acgaggccag ggcgggtgcg
     2341 atgtcggaca agaagggttc gagcgaaaag ctgctgtact gctccttctg cggcaagagc
     2401 cagcacgagg tgaagaagct gatcgccggc ccgtcggtct tcatctgcga cgagtgcatc
     2461 gacctgtgca acgacatcat ccgcgatgaa agcgccggcg agccggccgc cgccgccaag
     2521 tccgacctgc cgacgccgca ggagatctgc gcgatcctcg accagtacgt gatcgggcag
     2581 gaaaaggcca agcgcatcct gtcggtcgcc gtctacaacc actacaagcg gctgaagcac
     2641 ctgtccacca aggacgaggt ggagctcgcc aagagcaata tcctgctgat cggcccgacg
     2701 ggttcgggca agacgctgct ggcgcagacg ctggcccggc tgctgaacgt tccgttcgtg
     2761 atcgcggatg ccaccacgct gaccgaggcg ggctacgtcg gcgaggacgt cgagaacatc
     2821 atccagaagc tgctgcagaa ctgcaactac gaggtagaga aggcgcagaa gggcatcgtc
     2881 tacatcgacg agatcgacaa gatctcgcgc aagagcgaca acccgtcgat cacgcgcgac
     2941 gtgtcgggcg agggtgtgca gcaggcgctc ctgaagctga tcgagggcac cattgccagt
     3001 gtgccgccgc agggcgggcg caagcatccg aaccaggatt tcgtgcagat cgacacgacc
     3061 aacatcctgt tcatctgcgg cggcgcgttc gatggcctcg agaaggtcat ccgcaaccgc
     3121 tcggagaaaa gcggcatcgg cttcggcgcc gaggtgacga gtccgcgcga ccgctcggtc
     3181 ggcgatgtgc tgcgcgaggc ggagccggaa gacctgatca agttcggtct catcccggag
     3241 ctcgtcggcc ggctgccggt ggtggccacg ctcgatgaac tgagcgaaaa ggcgctggtc
     3301 gaaatcctgg tcgagccgaa gaatgcgctg atcaagcagt ttcgccgcct gctcgacatg
     3361 gaaggcgtgg agctcgaagt gcggccggac gcgctgcagg cgatcgccaa gaaggcgctc
     3421 aagcgtcgca cgggcgcgcg cggtctgcgc tcgatcgtcg aggcggtact gctcgacgtg
     3481 atgtacgacc tgccgggcat gcaaaacgtg atcaaggtgg tcatcgacga caacgccatc
     3541 gccggcggcc gaccgatcct gatctattcc gaccagccga aggtggccgg cgcgccgcgt
     3601 tgaggcccgc gccggccgcg tcggcgcttc ggtctaccgt caatgaccct gcgttttcgc
     3661 ggctttgcgc gccgcgagcg tgatagattg actctgctac tcggccgccc ttgcattttc
     3721 cccgggcggc cgcatttccc gggcatatct gccctcagga cgcgacatgt cgaccactct
     3781 gcttccttcc caacctactt cgctgccgct gctgccgctg cgcgacgtgg tcgttttccc
     3841 acacatggtc atcccgctgt tcgtgggccg gccgaagtcg atcaaggcgc tcgaaaccgc
     3901 gatggaggcg ggcaagagca tcatgctggt ggcgcagaag aatgcggcca aggacgagcc
     3961 gtcggccgag gacatctact cgatcggttg cgtggccaac atcctgcaga tgctgaagct
     4021 gccggacggg acgatgaagg tgctcgtcga gggggcgcag cgcgcgcaga tccatgagat
     4081 ccgcgacgcg cagacgcatt acacctgcga cctgacgccg atggcgcccg agacgtcggt
     4141 cagcccggaa ctcgaggcgc tgcggcgcgc gatcgtcgcg cagttcgacc agtacgtgaa
     4201 gctgaacaag aagatcccgg ccgagatcct gacctcgatc gccggcatcg acgaagccgg
     4261 ccggctggcc gacacgattg ccgcgcacct gccgctgaag atcgagcaga agcagaaaat
     4321 cctggagatg tcgagcctcg ccgaacggct cgagcacctg ctggcacagc tcgagacgga
     4381 gatcgacatc ctgcaggtgg agaaacgcat ccgcggccgc gtgaagcggc agatggagaa
     4441 gagccagcgc gagtactacc tgaacgagca ggtcaaggcg atccagaagg aactaggcga
     4501 gggcgaagag ggtgcggacc tcgaggacct cgacaagaag gtcaaggcgg cgcgcatgcc
     4561 gaagcaggcg cggcagaagg ccgagggcga gttgaagaag ctgaagctga tgtcgccgat
     4621 gtcggccgag gcgaccgtcg tgcgcaacta catcgacacg ctggtcaacc tgccgtggaa
     4681 gaagaagagc aagatcaaca acgatctggc caacgcgcag aaggtgctcg acgaggacca
     4741 ctacgggctg gacaaggtca aggaacggat cgtcgagtac ctcgccgtgc agcagcgcgt
     4801 cgaccgtctg aaggcgccga tcctgtgcct ggtcggaccg ccgggcgtcg gcaagacctc
     4861 actcgggcaa tccatcgcgc gcgcgaccaa ccgcaagttc gtgcgcacgg cgctcggcgg
     4921 cgtgcgcgac gaggccgaga tccgcggtca ccgccgcacc tacatcggct cgatgccggg
     4981 caagatcctg cagaacctct ccaaagtcgg cgtgcgcaat ccgctgtacc tgctcgacga
     5041 agtcgacaag atggggcagg acttccgtgg tgatccgtcg tcggcgctgc tcgaggtgct
     5101 cgatcccgag cagaaccaca cgttcgtcga tcactacgtc gaggtcgact acgacctctc
     5161 ggacgtgatg ttcgttgcca ccgcgaacac gatgaacatc ccgccgccgc tgctggaccg
     5221 gatggaagtg atccgcctgt cgggctacac ggaagacgag aagaccaaca tcgcgctgcg
     5281 gtatctgctg cccaagcaga tgcgcaacaa cggcgtgaag aaggaggagc tcgaagtctc
     5341 cgaaggcgcg gtgcgcgaca tcatccgcta ctactcgcgc gaagcgggcg tgcgtgcgct
     5401 cgagcgcgag ctgtcgaaga tctgccgcaa ggtggtcaag aacgcattgc tgaaggagag
     5461 tggcggcaag gtgaaggtca cgccgcgcaa tctcgacaaa ttcctcggcg tgcgccgcta
     5521 cacattcggc atggccgaga aggaagcgca ggtcggccag gtcacgggcc ttgcgtggac
     5581 cgaggtcggc ggcgaattgc tgaccatcga agcggcgcag atgccgggca agggcaacat
     5641 catccgcacg ggatcgctgg gcgacgtgat gaaggagtcg gtcgaggccg cacgctcggt
     5701 cgtgcgcagc cgctcgcggc gccttggcat caaggacgag gtgttcgaga agagcgacat
     5761 ccacattcac ctgcccgaag gagcaacgcc gaaggatggg ccgagcgcgg gcatcgcgat
     5821 gacgaccgca ttcgtatcga cgttgaccgg tattccggtg cggcccgacg tcgcgatgac
     5881 gggcgagatc acgctgcgtg gggaggtctt ggcgatcggc ggcctgaaag agaagctgct
     5941 cgccgcgcat cgcggcggta tcaagaccgt gctgattcca gaggaaaacg tcaaggatct
     6001 cgcagagata ccggacaacg tcaagaatcg actggaaatc attccggtga agtggatcga
     6061 ccgcgtgctg gagatcgcgc tacagcgcca gccggcgccg ctggccgagg acgaggccgc
     6121 ggcggcggcc gccgtcgcgc ccgccgctcc ggcgccggcc gcggccgacg gcaagttcgt
     6181 caagcactga gtccgcctta cgtatgcgcg cggatttgat ccgcgcgctt ttttttgacg
     6241 gattcgcagc atcgcgatgg atcggcttga cactcacatt cgcgcgcggt tgaatacatc
     6301 gctctgtgac tgatatccga tcggtgcgac tcggcatacg ccgaacaaga agctgataag
     6361 gacggcacga caactggcaa cccaacgagg gggtatacgt gaacaaaaca gaactgatcg
     6421 agcacattgc caagcaagct gacatctcca aagccgctgc gggacgtgcg ctcgaggcac
     6481 tggtcggagg gattcgggtt gcgctgaaga aaggcaattc cgtcacgctc gtcgggttcg
     6541 gcacgttcat ggtcggcaag cgcgccgccc gcaccggccg caacccgcgc accggcgcgg
     6601 cgatcaagat caaggcggcg aaggttccta agtttcgccc gggcaaggac ctgaaggatg
     6661 cggtaaactg aaattcttcg cagcgccggc acggacagtc ttgcagtcgc ggactcaggg
     6721 tgcttagctc agttggtaga gcggcgccct tacaaggcgt aggtcgggag ttcgagcctc
     6781 tcagcaccca ccaaccggct gtcaaggttg ctgcatgcga tgcagtcgtg cgaccgggga
     6841 gtggtagttc agttggttag aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc
     6901 cgtccactcc gccaacacac aaaggcgaac gcatgttcgc cttttttgtt gccgcctccg
     6961 gattgcagcc aacggccatg ttgaataggt cctgaagatg ttcgataccg tccgctcgca
     7021 tcgacgctgg ttgatgatct tcctggcggt gctggtcttc ccgtcgttcg tgatcaccgg
     7081 cgtctacagc tggaaccgct ttgtctcggc cgagaacgcg gtggcgacga tcggcagcga
     7141 gaagatctcg ccccagcagt tcgatgccgc gcatcgcgag cgcgtcgaac aactgcggca
     7201 ggcgctgggc gccaatttcg atcccaaggt attcgattcc gccgaagcgc gtgcggccgt
     7261 gctggacaac ctgctgtccg agcgcgcact ggcgcaggag gctgcccgcg atggcattgt
     7321 gatcagcgaa gcgcggctgc gcgaagtcat cgcctcggtg ccggcgtttc agcaggacgg
     7381 caagttctcg tacgaacgct acaagacgct gctcgcagcg caaggcatga acgagttcgc
     7441 tttcgagcaa cgcgtgcgcg acgatctgct gcgtcagacg ctgttgcggg cgatcggcca
     7501 gtccgcgttc gtgccgaagg ccgttctcga ccggatcaac cgactcggcg aggagcagcg
     7561 cgaggtgcgg ctgctgcgct tcgagcccaa ggattacgcg accaaggtga cggtcaccga
     7621 cgaggcgatc gacgcctact acaagggcaa ccgcgcgcaa ttcgagatcc ccgagagcgt
     7681 gaaggccgaa tacctggtgc tgacgctcga cgacgtcgct ggccagatcg cggtctcgga
     7741 ggccgaagtc aaggcgtatt acgagcagaa tcaggcgcgc ttcggcgagc ccgagcagcg
     7801 gcgcgccagt cacatcttgc tcacggtggg tgaaggcggc accgccacgg acaaggcggg
     7861 cgcgcggaag aaggcggaag aactgctggc gaagctgcgc gccaacccga attcgtttgc
     7921 gcaactggcg aaggagaact cgaaggatcc cggctcggcc gccagcggcg gcgacctcgg
     7981 cttctttggc cgcggcatga tggtcaaggc gttcgaggac gccgcgttca agctcaagga
     8041 aggcgagatc agcgacatcg tcgagtccga cttcggcttc cacatcatcc gggtgacggc
     8101 gatcaagccg gcgcaggtca agccgttcga acaggtccgc gccgagatcg agaaggacta
     8161 ccgccgccag caggcgcaga agaagttcgc cgaggccgtc gagcagttca ccaacacggt
     8221 ctacgagcag tcggacagcc tcaagcccgc agccgacaag ctcaagctcg cgatccagac
     8281 tgccgatcgg gtcacgcgtg aggggttgcc ggccaaaccc ggcgcgccgc agatcttcac
     8341 gccgcgagta ttgcaggcgc tcttctccga cgacggcatc aacaagaagc gcaacacgga
     8401 ggcgatcgaa gtggcaccga acacgttcgt gtccgcacgc gtggtcgagc atcgcccggc
     8461 gaccctgcgg ccgctggccg aggtgcgcga ttcgatcaag gcacgcgtcg agcggcagga
     8521 agccgcgcgc ctggcgcgcg aggcggccca gaaggcgctg gcggggctgc gccagtcgcc
     8581 gagcgcggcc ggcttcgcgc cgccggtcac gatttcgcgc gcgcaaccac agggcatgtc
     8641 gccgtcggcg ctcaccgcga tcatgcgcgt gccggcggac aagctgccga cgttcatcga
     8701 ggccgagctc gacggcggca gcgcggcgat cgtgcaggtg ctgagtgcga agatgcctga
     8761 cgcacccaac gtgcagcagc gcgaagcgtt cgcgcgcagc ctgcagcagc aggccggcac
     8821 cgccgatgac gttgcctatc tcgacgcgct gaaggccaag ttcaaggtcg aggtgttgaa
     8881 gccggatctg aagccggtcg cgacgccgca gggcgaagac aagaagaact gatcagcccg
     8941 atgcgatctc gcgcgccagg cgagcgaagt cgtcctcgga cgcgctcgcc ggcgagacga
     9001 ggcgagcgtc gccgacgcgc gcgaagtttc gtccgatcga cttgaggtag acggggtcgt
     9061 agtggtcgcg cagcagacgc tcgaccaact gcgcccagtc tccgtgctcg gcgagtcgtt
     9121 tccattcctg cacccggtcg cggccgtgca gcggcaccag gcagtcgagc tgccggaaca
     9181 gcgttgcgcg atcctgttcc aggtgcaggt actcgtcacg caacagcgcg atccggtccg
     9241 gcagggacag ctcgagccgc agacaatggc tggcgcgcat ccgatcgatc agcgcatcgg
     9301 gtacgcggag gtcgccgacc ttgcggctct cggactcgac gaacacggcg cgctccgcgt
     9361 cgaacccgcg cagtcgccac cagatgcggc tctcgaactg tttctgcgac ggctgcggtt
     9421 gccgcggcag tccgccgagc acggagccac gatgctgcgc caggttctcg aggtcgagca
     9481 cctgcgcccc ggcgcgttcg agctcacgca ggagacggct cttgccgctg ccggtggtgc
     9541 cgcagaccac gcggaactcg agccgcgccg gcaactccac caacgcctga acgactgcgc
     9601 gccgatacgc gcgatagccg ccctcgagct ggcgcgcatg ccaaccgaca cgcgccagca
     9661 cgtgcgtcat cgcgccgctg cgctggcccc cgcgccagca gtacacgagg ggcttccagt
     9721 cgcggggctt gccggcgaac gaggtctcca gatggcgagc gatgttgcgc gctaccagcg
     9781 ccgcgccacg ccgacgcgca atgaaaggag catcctgctt gtgcagcgtg ccgatcagtg
     9841 cgcgctccgc gtcgtcgagc acggcgcaat tgagcgcgcc tggcagatgg tcctcggcga
     9901 actccgattc actgcgcacg tcgatcagcg cgtcgaactc ttcgagccgc cgcagcgcct
     9961 cgtcgcacgc gatcacgctg cggtccatgt cacttcagta gtggccgcag tctgggccac
    10021 acgttgtcca gcatcagagg ctgcgcacgc tcgttcgggt ggatgcggtc ggcctgaaag
    10081 tagtcgagcc gatcggcgaa gccgtccagc aggaacggca ccaatgccgc cttctgtgca
    10141 cgcgcgactt cggcgaacgt ggcggcgaag cggtccgcgt agtcgcgtcc gtaattgggc
    10201 ggaatgcgca tgccgatcag cagcacgcgg gcgccaccag cacgactgag cgatgccatc
    10261 tcgctcaggt tctcgcgcag cgccgccagc ggcagcccgc gcaggccgtc attggcgccc
    10321 agttcgatca ccacgatcgc aggccggtgc tgcttcagca atgtcggcag gcgcgtgcgc
    10381 ccgccgctcg tggtttcacc gctgatgctc gcgttcacga cggtatactt ggcgcggcct
    10441 gtcgcaaggg tggcgagccg gttggcgagc agcttgaccc agccggtatc gcgcggcaaa
    10501 ccgtattcgg ccgacaggct gtcgccgaga accagaacgg acgggcgtac ctcggcgcgc
    10561 ggcgctccga atccgaacac acagcaaaac agcaccgcga tcgagacaat cgcgcgcgac
    10621 gccgccatac ccaatcgact catgcgggga actctgcgga atgcagaccg gaactgatgc
    10681 gttgcttgcg agcggcctga ccaagatcgt gcaggacgca acgggcagcc tgacgattct
    10741 cgatgatatc aatctgaaga tcgccgccgg cgaaacggtc gcgatcgtcg gtgcatcggg
    10801 ctctggcaag tcgacgctgc tcggactgct ggccggactc gatgtgccca cgcgcggcgg
    10861 ggtgctgctg ttcggcgagg atctgttcgc ccgcaatgag gacgaccgcg cgcgcgtgcg
    10921 cggcgcgaag atcggtttcg tattccagtc gtttcagctg ctgccgcacc tgaccgcgct
    10981 cgagaacgtg ctgctgccgc tcgagctcgc ggggcgcgcc cccgacggca tcgatccggt
    11041 cgatgcagcg aaggcgttgc tggagcgcgt cggactggcg gagcggctcg cgcactatcc
    11101 gaaatacctg tcgggcggcg agcagcagcg cgtcgcgctc gcgcgtgcat tcgtcagtcg
    11161 gccgccgctg ctgctcgccg acgagccgac cggcagcctc gacgcgcaca ccggcgaggc
    11221 ggtgatcgag ctgatgttcg agctgaatcg tgagcgggac tcgacgctgg tgctggtcac
    11281 gcacgatccg gacatcgccg cgcgctgcag ccggcgcatc gaactgcacg cgggccggat
    11341 tcggagcgac agcgcgtcag cggcggcgta accgcgccag gatgcgcgcc gcgatcggcg
    11401 caatcgcgcc ggcgacgagc ccgagttcat cgccaaactc gtccgcggcc gcgccgtgca
    11461 gccaggtcgc cgcgcacgtg gcgacgtcgg ccgcgaatct ctgtgcgacc agggcgccga
    11521 tcatgcccgc cagcgcgtcg ccggtgccgg cagtagcgag cgcgggcgtt ccggtcgggc
    11581 agatcccgta gcggccgtcg ggctgggcga cgagcgtgcc ggcgcccttc agaacgacga
    11641 tcgaaccgag tgcggccgca aggtcggtcg ccgcacggat gcggtccgac tgcacgtcgc
    11701 gcgcgccgcg gccgagcagc cgtgcagctt cgagcggatg cggcgtcagc acgcgcgggt
    11761 ggcgatggtt gcccagcgct ccgcgcagcg cctcgtcgcc cgcgatcaga ttcagcgcgt
    11821 cggcgtcgag caccagcggc gtgggctgca gcagcgcgaa acgcagcgca gccttcgcgc
    11881 cttcgtcggc gccgagaccg cagccgatca cgatcgcgtc gactggaatc acgtcggcgc
    11941 cgcggcggaa catcagctcg ggctgcaccg gatcgagccg catctcgggc gcgccgatgc
    12001 agtcgacgta gacacggccg gcgcccaggc gcagcgcggc gcgcgcggcg atcagcgccg
    12061 ccccgaccat gccaacgtgg ccgccgacaa ccagcgcgtt gccataggtt cccttgtgcg
    12121 tattgcgcgc gcgcggcgcg agcactgcgg cgaagtccga gggatcgagc aacgcgcccg
    12181 aggaggccgg cggcgcgatg cgcaggccgt cgacgatcac acggcccgcg gcgtcggcgc
    12241 cgtcgtgcgt gtacaggccc ggcttggcgc cgaggaacgt caccgtttcg ctcgcctgca
    12301 cgccggcgac gtcgcccacc cacgcgcccc ggtccgcgtc gagcccgctc ggcacgtcga
    12361 tcgcgaccac gcgcccgcgg cgtgccgaca tccagcgcgc cgcgtcgagg aacgcgccgg
    12421 caagcggacg cgccatgcca atgccgaaca tcgcgtccac gatcaggtcg aattccccgc
    12481 tgaccggcaa tgcaaaaata catgagccac cggcatcggc ccaacgcagc gcagcggcgc
    12541 gcgcgtcgtc ggccgccggc ggagcgagcg cgacgaccgt cacgcgatgg ccgagcgcgc
    12601 gcagttcggt cgcgcacaca tagccgtcgc cgccattgtt gcccggaccg cagacgatca
    12661 gcacgtgcgc gtcgtgccgg cgctcgtgaa tcagttgcgc cgcggccgtg ccggcgcggc
    12721 gcatcagttc gcccggggga agatctgcct gcaccgcggt ctcgatctgc cgcagcgccg
    12781 ccaccgtata gagcggaatg cccatggccg atgatcctac ccggaacgac gcgcgtaccg
    12841 gtccagcgtc aatccttcga gatcgatgtc aggcttgcga ttcgcgatca gatccgcgac
    12901 cacacgggcc gatccgcacg ccatggccca gcctgtcgaa ccgtgcccga cgttcaggtg
    12961 cagattcgcg atcggcgtgg gaccgaggag cggcgggccg tcgggcagca tcggccgcgc
    13021 gccgacccag aattgcgcct ggccgtaggc cgccgcgccg ggaaaccaat cgcgcgcgac
    13081 ccgcagcagc gttccgagcg atgcatcgcg cagtgtcagc gtgcgcgagc cgatctctgc
    13141 ggtcccggca atgcgcagcc gattacccat gcgcgtcacc gcgaccttgt aggtttcgtc
    13201 catcaccgaa atcatgggcg cgttttccgg tcgcgtgatc gccgccgtgg ccgaatatcc
    13261 cttgatcggg tagagcggca gccggatgcc gagcggacgc accagccgcg ggctgctgac
    13321 acccgccgcg accacgaccg catccgccgc cagcgtgccg tcgcgcaggc gcaggcgcgc
    13381 aacgcgcccg gcctcgactt cgagcgcgtc gacggcggtc ccgaagcgga actgcacgcc
    13441 ctgttcggcg ctgatgtcct tcatgcgctt ggcgaagaac gcgcaattgc cggtctcgtc
    13501 ctcgggcagg tgcaggccgc ccgcgagcgg cgtgccgtcg accaacgccg gctccaggcg
    13561 ccgacagtcc gctccgctga gcagcacgtg caacacgccg gcttccttca gcagcgcgcg
    13621 cgccagcgcg gtgcgcgcca gttccgcctc ggtgcggtag agctgcagat agccttgagt
    13681 ctgttcgtat tcgatgccgt gccgctggcg cagcgcatgc aggcagtcgc ggctgtagaa
    13741 cgcgaggcgc tgcatgcgcg ccttgttgcg gcggaagcgc tcgaccttgc attccgccag
    13801 ccatcgcgcc agccagcgcc actgcgcgaa atcgaagcaa ggccggaaca cgacgggcgc
    13861 ttcgcgcgcg aacagatgac gcagcacctt ggacggcatg cccggctgcg cccacggcgc
    13921 gatgtagccg ggcgcgatca cgccggcgtt ggcgaagctc gtctcctgcg ccacgccggg
    13981 attgcgctcg atgacggtga cttggaagcc ctgctggcgc aggtaatagg cggaagtgac
    14041 gccgatgatc ccggcgccga tcacggcgac gtgcatgcgg actcgaggtt cgcgctacag
    14101 cctcatgcgg ctcggcagga aggtcaccag ttgcgggaag atgtagatga gcacgacggc
    14161 cgccaccatc aggaggaaga acggcagcgt cacgcgcgcg atgtagccga tctcgcgctt
    14221 cgtcattccc tgcagcacga acaggttgaa gcccacgggc ggcgtgatct gcgccatctc
    14281 gaccaccagc acgacgaaga tgccgaacca gatcaggtcg atgccggccg cggtcaccgt
    14341 cggcagcagc acgcccatcg tcagcacgac catcgagatg ccgtcgagga agcagccgag
    14401 caggatgtag aacagcgtga gcgcgacgat cagccacacc ggtgacaggc cgagcgaagc
    14461 aatgaattcg gcca
//
LOCUS       DBNHBY010000357         7614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_766269, whole genome shotgun sequence.
ACCESSION   DBNHBY010000357 DBNHBY010000000
VERSION     DBNHBY010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_766269"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1318)
                     /locus_tag="AC16HB_08830"
     CDS             complement(5..1318)
                     /locus_tag="AC16HB_08830"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5606933.1"
                     /translation="MTPSNQKATLTFTDGTPPLDLPIYKGTIGPDVIDIRQLYTKTAK
                     FTYDPGFLSTASCNSSITYIDGDKGELLYRGYPIDQLATQCDFLEVCHLLLYGELPNA
                     AQKADFTARVTRHTMVNEQMQFFLRGFRRDAHPMAVMTGLVGALSAFYPDSINLHDAH
                     QRDISAVRLIAKMPTLVAMAYKYSVGQPYMYPRNELSYAGNFMRMMFATPCEDFKPNA
                     VLVRAIDRIFTLHADHEQNASTSTVRLCASSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLYDIQRNGGAAKIGEFIAQVKDKNSNVKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     GELGLHDDPLFKLAMALEKIALEDDYFVSRKLYPNVDYYSGIVQKAIGIPVPLFTAIF
                     ALARTVGWIAQLNEMIADPEYKIGRPRQLFVGSTHRRVLPLHQRV"
     gene            complement(1360..1623)
                     /locus_tag="AC16HB_08835"
     CDS             complement(1360..1623)
                     /locus_tag="AC16HB_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HIR5606934.1"
                     /translation="MVAPVGDPERRRLRWRARRGLLENDLLLTRFLDRCGDDLDGDAA
                     AALAQLLALPDNDLLDLLLSRKNPAGALDTIAVRELIERIRTA"
     gene            complement(1626..2384)
                     /locus_tag="AC16HB_08840"
     CDS             complement(1626..2384)
                     /locus_tag="AC16HB_08840"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR5606935.1"
                     /translation="MTASNPSENGAVAVAAGARTVKFEVYRYDPDRDVKPYMQKVEVR
                     LQPTDRMLLDALMRLKADFDDSVAFRRSCREGVCGSDAMNVNGKNRLACITNLNELKE
                     PIVLKPLPGLPVIRDLIVDMTQFFKQYHSIKPYLINDTPPPEKERLQSPEEREELNGL
                     YECILCACCSTACPSFWWNPDKYVGPAGLLQAYRFIVDSRDQATGERLDDLHDPYRLF
                     RCHTIMNCTDVCPKGLNPAEAIGRIKTMLTRRAI"
     gene            complement(2395..4161)
                     /gene="sdhA"
                     /locus_tag="AC16HB_08845"
     CDS             complement(2395..4161)
                     /gene="sdhA"
                     /locus_tag="AC16HB_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR5606936.1"
                     /translation="MALARRRFDAVIVGAGGAGMRCSLQLAQAGLNVAVLSKVFPTRS
                     HTVAAQGGIAATLANMNEDQWLWHMFDTVKGSDYLGDQDAIEFMCREAPKVVYELEHF
                     GMPFDRNPDGSVYQRPFGGHTANYGEKPVQRACAAADRTGHALVHTLYQQNVKARTQF
                     FVEWMALDLIMNADGECVGVIALEMETGEPMILEGKVTVLATGGAGRIFEASTNAYIN
                     TGDGLGLVARAGLPLQDLEFWQFHPTGVAGAGVLITEGVRGEGGILLNSNGERFMERY
                     APTYKDLAPRDFVSRSMDQEIKEGRGCGPHKDHVLLKLDHLGAETIMKRLPGIRDISI
                     TFANVDPIKEPIPVVPTLHYQMGGIPANFHGEVVAPRNGNPDAVVPGLYAIGECSCVS
                     VHGANRLGCNSLLDLIVFGRAAGNHIVESLKKEPRENKLLPAGAGENALARLAALDAR
                     EKGERTQDVANGIRRTMQAHCGVFRTGKLLDEGVARIKELGERARHVAIADKSKVFNT
                     ARIEALELANLVETAMASIVSAAARTESRGAHARDDFPERDDANWLKHTLWYSEGRRL
                     DYRPVHMKPLSVDSFAPKKRTF"
     gene            complement(4163..4525)
                     /gene="sdhD"
                     /locus_tag="AC16HB_08850"
     CDS             complement(4163..4525)
                     /gene="sdhD"
                     /locus_tag="AC16HB_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR5606937.1"
                     /translation="MASNVGSKRLVVGAHYGLRDWLAQRLTAVLMAIYAIVLGIYAIF
                     VLPPGYDAWAGAFAPLSMKVATLVVMFALLYHAWVGMREIFMDYLKPTWVRLVAYTVT
                     LVWLAVCASWSVQILWRV"
     gene            complement(4526..4939)
                     /gene="sdhC"
                     /locus_tag="AC16HB_08855"
     CDS             complement(4526..4939)
                     /gene="sdhC"
                     /locus_tag="AC16HB_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR5606938.1"
                     /translation="MSSPLASTKRPEFRNIHVTQIIRYRLPVGGFASFLHRFSGALMF
                     LALPLLLWLFDLSLTSEVSYMRLAALFSHWFVKLILLGLSWALLHHLCAGVRHLLLDL
                     HLGVEKHAAERSAWTVYLISLPLTLVAALKLFGVF"
     gene            complement(5041..5808)
                     /locus_tag="AC16HB_08860"
     CDS             complement(5041..5808)
                     /locus_tag="AC16HB_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5606939.1"
                     /translation="MEVDVDPTADASARRGRGAVFAPLYRQIKQLLVASLEQREWKPG
                     ELIPSEIELAARFQVSQGTVRKAVDELAAANLLVRRQGKGTFVATHHEPRAQFRFLRI
                     VPDTGEPVPAESRFLECRRLKAPAEIAHQLQLKTGDPVVFVRRLLSFSGKPVVLDDIW
                     LPGALFKGLTADRLARYHGPLYGLFESEFDTRMIRAQEQLRAVAADAMVARLLQVPKE
                     SPLLQVDRVSFSYGDKAVEMRRGLCVTTAHHYRNALY"
     gene            6090..7079
                     /locus_tag="AC16HB_08865"
     CDS             6090..7079
                     /locus_tag="AC16HB_08865"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5606940.1"
                     /translation="MTKAPVRVAVTGAAGQIGYSILFRIASGQMLGADQPVILQLLEI
                     PDEKAQKALKGVMMEMDDCAFPLLAGMSAHSDPMAAFKDVDVALLVGARPRGPGMERK
                     DLLTANAQIFTAQGKALDAVASRRVKVLVVGNPANTNAYIAMKSAPSLPKKNFTAMLR
                     LDHNRALSQIAARTGKPVASIEKLCVWGNHSPTMYPDYRFATIDGKSVKDMINDENWN
                     RTAFLPTVGKRGAAIIEARGVSSAASAANAAIDHIRDWVLGTGGKWVTMGIPSDGSYG
                     IPADTMFGFPVTCANGEYSLVRNLPIDEFSQQCINKTLAELNEEKDGVKHLLA"
     gene            7089..7457
                     /locus_tag="AC16HB_08870"
     CDS             7089..7457
                     /locus_tag="AC16HB_08870"
                     /inference="COORDINATES: protein motif:HMM:NF021390.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HIR5606941.1"
                     /translation="MRSLRFALLIGAAAACASAAAEAPAPARVGKDWPITRQWKCETG
                     RELLLNFNPRRIKRAAWLTYGGNRAEVYRVPAASGVAYASKDGKVKWHEQGEDGTVEY
                     AGVIDRPVQCALVKPERKKK"
BASE COUNT         1286 a         2545 c         2485 g         1298 t
ORIGIN      
        1 ccctctacac ccgctggtgc agtggcaaca cccggcggtg cgtgctgccg acgaagagct
       61 gccgcgggcg gccgatcttg tattccggat cggcgatcat ctcgttcagt tgcgcgatcc
      121 agccgaccgt gcgcgccagc gcaaagatgg cggtgaacag cggcaccggg attccgatcg
      181 ccttctgcac gatgcccgag tagtagtcga cgttcggata cagcttgcgg ctgacgaaat
      241 agtcgtcctc gagcgcgatc ttctccagcg ccatcgcgag cttgaacaac gggtcgtcgt
      301 gcaggcccag ttcgcccagc acttcgtggc aggtctcgcg catcagtttc gcgcgcgggt
      361 cgtagttctt gtacacgcgg tggccgaatc ccatcagctt gacgtttgaa ttcttgtcct
      421 tcacctgcgc tatgaattcg ccgatcttcg ccgcgccgcc attgcgctgg atgtcgtaca
      481 gcatgttcag gcacgcctcg ttggcgcccc cgtgcgcggg gccccacagg cacgcgacgc
      541 cggccgcgat cgcggcgaat ggattggtgc cgctggaggc gcacaggcgt acggtcgagg
      601 tcgacgcgtt ctgctcgtgg tccgcgtgca gcgtgaagat gcggtcaatc gcgcgcacca
      661 gcacggcatt cggcttgaag tcctcgcacg gcgtggcaaa catcatgcgc atgaagttgc
      721 cggcatacga cagctcgttg cgcgggtaca tgtagggctg gccgaccgag tacttgtacg
      781 ccatcgcgac cagcgtgggc atcttggcga tcagccggac tgccgagatg tcgcgctggt
      841 gcgcgtcgtg caggttgatc gagtccggat agaacgcgct gagcgcaccg accaggccgg
      901 tcatgaccgc catcgggtgg gcgtcgcggc ggaacccgcg caggaagaac tgcatctgct
      961 cgttgaccat cgtgtgcctg gtcacgcgcg cggtgaaatc ggccttctgc gcggcattcg
     1021 gcagttcgcc gtacaagagc agatggcaga cctcgaggaa gtcgcactgg gtggcgagct
     1081 ggtcgatcgg atagccgcgg tacagcagtt cgcccttgtc gccgtcgatg taggtgatgc
     1141 tcgagttgca cgaggcagtc gacaggaagc ccgggtcgta ggtgaacttg gcggtcttgg
     1201 tgtatagctg gcgaatgtcg atcacgtcgg gaccgatcgt gcccttgtag atcggcaggt
     1261 cgagcggcgg cgtgccgtcg gtaaaggtca gtgtggcctt ctgattggag ggggtcagtg
     1321 gcatcgtcgt cttccttcgt tcggttgggc gcgatcaagt caggccgtac ggatgcgttc
     1381 gatcagttca cgcacggcga tcgtatcgag ggcgccagcc gggtttttgc gcgaaagcag
     1441 aagatcgaga agatcgttgt cgggcagcgc cagcagctgc gccaaggcgg ccgccgcgtc
     1501 gccgtcgagg tcgtcgccgc agcggtcgag aaagcgcgtc agcaacaggt cgttctccag
     1561 cagtccgcga cgcgcgcgcc agcgcagccg gcgccgctcc ggatcgccga ccggcgcgac
     1621 cataatcaga tcgcccgccg cgtcagcatc gtcttgatcc tgccgatagc ctccgccggg
     1681 ttcagcccct tcgggcagac gtcggtgcag ttcatgatgg tgtggcagcg gaacagccga
     1741 tacggatcgt gcaggtcgtc gagccgctcc ccagtggcct ggtcgcggct gtcgacgatg
     1801 aagcggtagg cctgcagcag cccggccggg ccgacgtact tgtccgggtt ccaccagaag
     1861 ctcgggcacg ccgtcgagca gcatgcgcac aggatgcact catacaggcc gttcagctcc
     1921 tcgcgctcct ccggcgactg caggcgctcc ttttccgggg gcggcgtgtc gttgatcagg
     1981 tacggcttga tcgagtggta ctgcttgaag aactgcgtca tatccacgat caggtcgcgg
     2041 atcaccggca gtcccggcag cggcttcagg acgatcggct ccttcagctc gttcaggttc
     2101 gtgatgcagg ccagccggtt cttgccgttg acgttcatcg cgtcggagcc gcacacgccc
     2161 tcgcggcacg agcggcggaa cgcgacgctg tcgtcgaagt cggccttcag ccgcatcagc
     2221 gcgtcgagca gcatccggtc ggtcggctgc agcctgacct cgaccttctg catgtagggc
     2281 ttcacgtcgc ggtcggggtc gtagcggtag acctcgaact tcacggtgcg cgcgccggcc
     2341 gcgaccgcga cggcgccgtt ctcgctggga ttgctggcgg tcatcgttcc gtcctcagaa
     2401 agtccgcttc ttgggcgcga agctgtcgac ggacagcggc ttcatgtgca ccggccggta
     2461 gtcgaggcgc ctgccttccg aataccacag cgtgtgcttc agccagttcg cgtcgtcgcg
     2521 ctccgggaaa tcgtcgcgcg cgtgcgcacc gcggctctcg gtgcgcgccg cggccgagac
     2581 gatcgaagcc attgccgttt cgaccagatt ggcaagctcg agcgcctcga ttcgcgcggt
     2641 gttgaagacc ttggacttgt ccgcgatcgc cacgtgcctg gcgcgctcgc cgagttcctt
     2701 gatcctggcc acgccctcgt cgagcagctt gcccgtgcgg aacacgccgc aatgcgcctg
     2761 catcgtccgg cgaatgccgt tggcgacgtc ctgcgtgcgc tcgcccttct cgcgcgcgtc
     2821 cagcgcggcc agacgcgcca atgcgttttc gccggcgccc gccggcagca gcttgttctc
     2881 gcgcggctcc ttcttcagcg attcaacgat gtggttgccc gcagcacggc caaagacgat
     2941 caggtcgagc agcgagttac atccgagccg gttggcgccg tgcacgctga cgcacgaaca
     3001 ttcgccgatc gcgtacagcc ccggcacgac cgcatccgga ttgccgttcc tcggcgcgac
     3061 gacctcgccg tgaaagttgg ccgggatgcc gcccatctgg tagtgcagcg tcggcacgac
     3121 cgggatcggc tccttgatcg gatcgacatt ggcaaaggta atcgagatgt cgcggatgcc
     3181 cggcagccgc ttcatgatcg tctcggcgcc gaggtggtcg agcttcagca gcacatggtc
     3241 cttgtgggga ccgcagccgc gtccttcctt gatctcctgg tccatcgatc gcgacacgaa
     3301 gtcgcgcggc gccaggtcct tgtaggtcgg tgcgtagcgc tccatgaagc gctcgccgtt
     3361 gctgttcagc agtatcccgc cctcgccgcg cacgccttcg gtgatcagca cgccggcccc
     3421 ggccacgccg gtcggatgaa actgccagaa ctccaggtcc tgcaggggca atcccgcgcg
     3481 cgcaaccaga cctagaccgt cgccggtgtt gatgtacgcg ttggtcgacg cttcgaaaat
     3541 gcggccggcg ccgccggtgg cgagcaccgt gaccttgcct tcgaggatca tcggctcgcc
     3601 ggtttccatt tccagcgcga tcacgccgac acattcgccg tcggcgttca tgatcaggtc
     3661 gagcgccatc cactcgacga aaaactgcgt gcgtgccttg acgttctgct ggtacagggt
     3721 atgaaccagc gcgtgcccgg tgcgatcggc cgcggcacag gcgcgctgca ccggtttctc
     3781 gccatagttc gcggtgtggc caccgaaggg acgctggtag accgagccgt ccgggttgcg
     3841 atcgaacggc atgccgaaat gctcgagttc gtacacgacc ttcggcgctt cgcgacacat
     3901 gaactcgatc gcgtcctggt cgcccaggta atcggagccc ttgaccgtgt cgaacatgtg
     3961 ccacagccat tgatcttcgt tcatgttggc gagcgttgcc gcgatgccgc cctgcgccgc
     4021 caccgtgtgc gagcgtgtcg gaaacacctt cgacagcacg gccacgttga gccccgcctg
     4081 cgccagctgc agcgagcagc gcatgccggc gccgcccgcg ccgacgatca ccgcatcgaa
     4141 tcgacgtcgt gcgagtgcca tctcagactc tccagaggat ctgcaccgac catgatgcgc
     4201 aaacggcgag ccagaccagc gtgaccgtat aggcaaccag ccgcacccac gtcggcttca
     4261 ggtagtccat gaagatctcg cgcatgccga cccacgcgtg gtacagcagc gcgaacatca
     4321 cgaccagggt ggcgactttc atcgacagcg gtgcgaacgc gccggcccac gcgtcgtaac
     4381 cgggcggcag gacgaatatc gcgtagatgc cgagcacgat cgcgtagatc gccatcagca
     4441 ccgcggtcag gcgctgcgcc agccagtcgc gcagtccgta gtgggcgccg acgacgaggc
     4501 gcttggaacc gacgttggaa gccatctaga acactccgaa caacttgagc gccgccacca
     4561 gcgtcagcgg caggctgatc aggtagaccg tccacgcgct gcgttccgca gcgtgcttct
     4621 cgacgcccag gtgcaggtcg agcagcaggt gccgcacgcc ggcgcagagg tggtgcagca
     4681 gggcccagct caacccgagc aggatcagct tgacgaacca gtgcgagaag agcgctgcca
     4741 gccgcatgta gctcacctcg gacgtcaggc tgaggtcgaa cagccacagc agcaggggca
     4801 gcgcgaggaa catcagggcg ccactgaacc gatgcaggaa cgacgcgaac ccgccgaccg
     4861 gcaagcggta gcggatgatc tgcgtcacgt ggatgttgcg gaattcgggg cgcttggtcg
     4921 aggccagagg ggagctcatc gttcgtgtcg ttgatgtggc attgcgccga tcgcgtgcag
     4981 gggaccgtcc gctattctgg ccgaagttgc tgtatcggaa caaatcgagg cggcgccgcc
     5041 tcagtacaac gcgttgcggt aatgatgcgc ggtcgtcacg cacaggccgc gccgcatctc
     5101 gactgccttg tcgccgtacg agaacgacac gcgatcgacc tgcagcagcg gggactcctt
     5161 cggcacctgc agcaggcgcg cgaccatcgc gtccgctgcc accgcgcgca attgctcctg
     5221 cgcgcggatc atccgcgtgt cgaactccga ttcgaacaga ccgtacagcg gcccgtgata
     5281 gcgggcgagc cggtccgcgg tcaacccctt gaacagtgca ccgggcagcc agatgtcatc
     5341 gagcacgacc ggcttgcccg agaagctgag cagccgccgc acgaagacga ccggatcgcc
     5401 cgtcttcagc tgcagttgat gcgcaatctc cgccggcgcc ttcagccgcc ggcactccag
     5461 gaagcgactt tcggccggca ccggttcgcc cgtgtccggc acgatgcgca ggaagcggaa
     5521 ctgcgcccgc ggctcgtgat gggtggcgac gaacgtgccc ttcccctgtc gccgcaccag
     5581 caggttcgcg gccgccagct cgtccaccgc cttgcgcacc gtgccctggc tgacctgaaa
     5641 gcgggcggcg agttcgatct cgctcgggat cagctcgccc ggcttccatt cgcgctgctc
     5701 caggctcgcc accagcagct gcttgatctg ccggtacagc ggtgcgaaca ccgcgccgcg
     5761 cccgcgtctc gcgctggcgt cagcggtggg atcgacatcc acttccatgg cgcgatttca
     5821 gcacagattc ccggaccgcg ggcagtatct tatgtcttat ataagagtta tactttgatc
     5881 atgcgcaagc ccgcccgtcc acccgcggcc gaccgcacgc ccgcacgccg gcactgcgcg
     5941 cgatcaaaca gtgcgccccg ccgcggggat tacactgccc gccacgtgcc gcccggcccg
     6001 cgcgcgcagc gcgggcgcgg cgtagccgac ccttgccgct gtccgcatcc gcgacccgcg
     6061 atcatcctcg acccacccgg agaagtccca tgacgaaagc tcccgttcgc gtcgccgtca
     6121 ccggcgccgc cggccagatc ggttactcca tcctgttccg catcgcttcg ggccagatgc
     6181 ttggcgccga ccagccggtg atcctgcaac tgctcgagat ccccgacgag aaggcgcaga
     6241 aggcgctcaa gggcgtgatg atggagatgg atgactgcgc cttcccgctg ctggccggca
     6301 tgagcgcgca cagcgacccg atggcggcgt tcaaggacgt cgacgtggcg ctgctggtcg
     6361 gcgcgcggcc gcgcgggccc ggcatggagc gcaaggatct cctgacggca aacgcgcaga
     6421 tcttcaccgc gcagggcaag gcgctcgacg cggtcgcctc gcgccgcgtc aaggtgctgg
     6481 tcgtcggcaa ccccgccaac acgaacgcct acatcgcgat gaaatcggcg ccgagcctgc
     6541 cgaagaagaa cttcacggcg atgctgcggc tggatcacaa ccgtgcgctg tcgcagatcg
     6601 cggcccggac cggcaagccg gtggcctcga tcgagaaact gtgcgtgtgg ggcaaccact
     6661 cgccgacgat gtaccccgac taccgcttcg cgaccatcga cggcaagtcg gtcaaggaca
     6721 tgatcaacga cgagaactgg aaccgtaccg cgttccttcc gaccgtgggc aagcgcggcg
     6781 ccgcgatcat cgaagcgcgc ggcgtttcgt cggcggcgtc agccgccaat gcggcgatcg
     6841 accacatccg cgactgggtg ctcggcaccg gcggcaagtg ggtgacgatg ggcattcctt
     6901 cggacggctc gtacggcatt ccggccgaca cgatgttcgg cttcccggtc acctgcgcca
     6961 acggcgagta ttccctcgtc cgcaacctgc cgatcgacga gttctcgcag cagtgcatca
     7021 acaagacgct ggccgagctg aacgaggaga aggacggcgt caagcacctg ctggcctgag
     7081 cgcacccgat gcgatcgttg cgattcgcac tgctgatcgg cgccgcggcc gcgtgcgcga
     7141 gcgcggccgc cgaggcgccc gcgccggcgc gcgtcggcaa ggactggccg atcacgcgac
     7201 agtggaaatg cgaaaccggc cgcgagctcc tgctgaactt caatccgcgc cggatcaagc
     7261 gagcggcgtg gctgacctac ggcggcaatc gcgccgaggt ctatcgcgtg cccgccgcgt
     7321 cgggcgtcgc ctacgcaagc aaggacggca aggtgaagtg gcacgagcaa ggcgaggacg
     7381 gaacggtcga gtatgccggc gtgatcgacc ggcccgtgca atgcgccctc gtcaagccgg
     7441 aacggaagaa gaagtaggtc cgcgccgcgg attgctcgtc ccgtggtcgc gccctcctct
     7501 accgtccgtc cgcagcccga tcgggtcctg ctcgacatcg ccgactacgt gagccgttat
     7561 cgggtccgca gccgcaccgc ttacgacacc gcgcggctgt gcctgatgga tacg
//
LOCUS       DBNHBY010000358         6438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1307569, whole genome shotgun sequence.
ACCESSION   DBNHBY010000358 DBNHBY010000000
VERSION     DBNHBY010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1307569"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC16HB_08875"
     CDS             complement(<1..385)
                     /locus_tag="AC16HB_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5606942.1"
                     /translation="MLPQRRVLVIEDHQEIAALVRLHLLDLPCDVELAYDGAKGLALA
                     EAKPFDLVILDLMLPGLDGLEICKRLRARQNYTPILMITSKSSEFDRVLGLELGADDY
                     LAKPFSVIELMARVKAIFRRVDALAK"
     gene            535..6255
                     /locus_tag="AC16HB_08880"
     CDS             535..6255
                     /locus_tag="AC16HB_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5606943.1"
                     /translation="MPDTVVALQYELTEELAGDERFRLYRGRTSGGDSVLVKLPAQQP
                     AFADDIARLKREYEILRRPPVDRATRALDLVCTDHHCALVLEDRGDRPLAALIAARAT
                     PIGWALDYALQIAATLAALHRAGIVHRALSPRSVLVQDREGRIRLTDFSDAARDAAEA
                     CVPLAEIQYRTRLPYAAPEQTGRVNRVCDWRTDFYSLGALLYEMLAGRPPFTANDPLE
                     LMHAQIARIPSAPAEIAAVPLPLSRIVMKLLAKQPEERYQSARGLQQDLERCRREWSE
                     RGRIADFPIAAADVSDRFSVPQRLYGRDAERAALLDAFDAARRGMTSLLLVEGPAGIG
                     KTALMQVLHEPVSRCRGNFVSGKFDQLSRDVPYAALVRALRQCVQRLLAEAPEQLARD
                     GARLDASLGPNAAVIAEVIPEIELILGPQRPAPPLPPAEAHNRFTLAFQNFVAALATA
                     DAPLAIFLDDLQWADASTLQLLQPLLTSPQIHHLLLIGAWRANDVDPDHPLPPALAAL
                     TAAGVDFRRIALAPLALPALIELIADTLHAPHERAQPLARLLLERTGGNPFFATQFLT
                     TLHRDGLIVFDPERAAWQCRLDAITESGDDDVADLMSRKLERLPADARSALTLAACLG
                     NRFDAGTLARVCDRSSRAVLADLDAAVAEGLLVPEANQAFAFLHDRVQQAAYARLSAD
                     DRARLHLQIGRALATDATATGHDASGFDRVFDAASHLNLGARLIDAPEEKLALARLNL
                     AAGDKARTSAAYRSALGYLTAGIALLDACHWGSEYELAFTLHLEAAQCEYLCGDFDTA
                     LRQYAALLQRAASDLDRARVHRLRAIQFENMARYTDALDAVRAGLAALGIALPASARA
                     RRSALAREIAQIRARLAGRTIASLIDLPPMIEPSMRMVMKLLTDAWSSAYIQGDATLA
                     RLISAMLVRLSLVHGNAEESAYGYVTHAITAGPVQGDYDEAWQWGALALQVNERLEDR
                     RLRAKVYQQFHAHVNLWCRPFAACVQYAKEACRAGLESGDFLYAAYGASTESWPAFAA
                     AQDLAGFVADLQPNLALLTRLKNDSFADALRLMIAWSLALQGAAMDAPRSLSNPDFDE
                     DRYVETYRGNAFFSMFHAIARLAVCFLLDDAAGAASAARSVQSTAHELIGMLWSVQFW
                     FWNGMRLAEAYAGADAAGRATALKELRAARRTLDALAESCPENFRCSALLLAAEQARI
                     TERPFEALDLYQRAIAYAEEVAQIQHRALASERCGLLWLERGNRDVAAHYLRAARDGY
                     AQWGAQAKVKRLLQRHGELLGASERIASAASVPPRAPESLDIASIGKAAHALAADVEL
                     DTLIRRMLRIAIESAGASRGALVEVQDGSARVVADGSAESDSGIVLRDAPLDANPRTC
                     SPAIVNYVLRTQAALVLSEPLLDERFAHDEYLAGARPKSVLCLPIVQQRGMRAVLYLE
                     NGLVRDAFGAERIEVIQILASQAASALHSARLLDRMQREMHERERAEQQLRAIEAGTS
                     TVTGGDFFRALVRNLALALRVRYVFVAECLRDDPGRGRIVRSRAFWNGDDFGDNFEFA
                     VPGTPCQHVIEGEVCHYAEDLQARFPEDAGLARWDAHSYLGMPLVDSGGTVIGHVAVL
                     DTEPMPDATLARAVLRLSAGRAGAELERLKAHEGLQRALVKVEELKNRLQEENVYLRR
                     ELIANVSHDLRSPLASLRGYLDTLLLKEQTLSAAERRSFLSIAQRQAEHLQTLISELF
                     DLARLDFDGYRIDAEPVHLGELARDVLQKFQLAAQKKHVQLALAIDDDLGFVSADIGL
                     LERTLENLLENALAHTPAAGRVELAVLAHEVGALIQVSDTGRGIPPDDLPRIFERFYR
                     VDKARTLNSSGSGLGLAIVKRIVELHGSRIHVESNTGRGTRFWFTLQTAAAAASQPTV
                     TTERPA"
     gene            complement(6233..>6438)
                     /locus_tag="AC16HB_08885"
     CDS             complement(6233..>6438)
                     /locus_tag="AC16HB_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR5606944.1"
                     /translation="VAEERTVKGSYIGTCVPLRDLPRYIALHRQGRLPVRRLLSGTMT
                     LDESNEGFDRLREGRAIRQVVLL"
BASE COUNT          976 a         2289 c         2198 g          975 t
ORIGIN      
        1 gcttcgccag cgcgtcgacc cggcggaaga tcgccttcac gcgcgccatc agctcgatca
       61 cggaaaacgg cttcgccaga tagtcgtcgg cgccgagttc gagtccgagc acgcggtcga
      121 actccgacga cttcgacgtg atcatcagga tcggcgtgta gttctgccgc gcgcgcagcc
      181 gcttgcagat ctccagtcca tcgagtcccg gcagcatcag gtccaggatc acgagatcga
      241 atggcttggc ctccgccagc gccagcccct tggcaccgtc gtaggccagc tcgacatcgc
      301 acggcaggtc gagcaggtgc agccgcacca gtgcggcgat ctcctgatgg tcctcgataa
      361 ccagcacccg gcgctgcggc agcacttcgc ttcctcgtct ggaccgcgac gcccgattgt
      421 gccgccagcg ccgcggcgga agttcaaatt tccatcacat cgcgtgacgg ctgcttgcgc
      481 cgccggcccc gcactcctaa tgttgccccg ctgccgctca gcgtccatcc cgccgtgccc
      541 gacaccgtcg tcgcgttgca gtacgaactc accgaggaac tcgcgggcga cgagcgcttc
      601 cgcctgtacc gcggccgaac gtccggcggc gactccgtgc tggtcaagct gcccgcgcag
      661 cagccggcgt tcgccgacga catcgcgcgc ctgaagcgcg aatacgaaat cctgcgccgg
      721 ccacccgtcg atcgcgccac gcgcgcgctc gatctcgtct gcaccgacca tcattgcgca
      781 ctggtgctcg aggatcgcgg cgatcgcccg cttgcagctc tgatcgccgc gcgtgcgacg
      841 cccatcggct gggcgctgga ttacgcgctg cagatcgcag cgacgctggc cgcgctgcat
      901 cgcgccggca tcgtgcatcg cgcactcagc ccgcgctcgg tgctggtgca ggatcgcgag
      961 ggtcgcatcc ggctcaccga cttctccgac gcggcgcgcg acgcggccga agcgtgcgtc
     1021 ccgctcgccg aaatccagta ccgcacgcgg ttgccgtatg cggcgccgga gcagaccggg
     1081 cgcgtcaatc gcgtgtgcga ctggcgcacc gatttctatt ccttgggcgc gttgctgtac
     1141 gaaatgctcg ccggacgccc accattcacg gccaacgatc cgctcgagct gatgcacgcg
     1201 cagatcgcgc gcatcccgtc cgcaccggcc gagatcgccg ccgtgcccct gccgttatcg
     1261 cgcatcgtga tgaagctgct cgccaagcag cccgaagagc gctaccagag cgcgcgcggg
     1321 ctgcagcagg acctggagcg ctgccggcgc gaatggtcgg agcgcggacg catcgcggac
     1381 tttccgatcg cggcggccga cgtatcggac cgcttctcgg tgccacagcg cctgtatggg
     1441 cgcgacgccg agcgcgcggc gctgctggac gcgttcgacg cggcacgtcg gggaatgacc
     1501 tcgctgcttc tggtcgaagg gccggccgga atcggcaaga ccgcgctgat gcaggtcctg
     1561 catgagccgg tgtcgcgctg ccgcggcaat ttcgtcagcg gcaagttcga tcagctgtcg
     1621 cgcgacgtgc cgtacgccgc gctggtccgg gcgttgcgcc agtgcgtgca gcgcctgctg
     1681 gcggaggcac cggagcaact ggcgcgcgac ggcgcgcggc tcgacgccag cctcggtccg
     1741 aatgccgcgg tgatcgccga ggtgatcccc gagatcgagc tgatcctcgg cccgcagcgg
     1801 cccgcgccgc cactgccccc ggccgaagcg cacaaccgct tcacgctcgc gttccagaat
     1861 ttcgtcgccg cgctcgccac cgcggacgcg ccgctggcaa tcttcctcga cgacctgcag
     1921 tgggccgatg cctcgacgct gcagttgctg cagccgctgc tgaccagccc gcagatacac
     1981 catctgctgc tgatcggcgc ctggcgtgcg aacgacgtcg accccgatca tccgctgccg
     2041 ccggcgctgg cagcgctgac ggccgcgggt gtcgatttcc ggcgcatcgc gctcgcgccg
     2101 ctggcactgc cggcgctgat cgaactgatc gccgatacgc tgcacgcacc gcacgagcgc
     2161 gcccagcccc tggcacgact gctgctggag cgaacggggg gcaatccgtt cttcgccacc
     2221 cagttcctga ccacgctgca ccgggatgga ctgatcgtgt tcgatccgga gcgggcggcc
     2281 tggcaatgcc ggctcgacgc gatcaccgag tcgggcgacg acgacgtggc cgacctgatg
     2341 agccgcaagc tcgagcgtct tccggccgac gcccgcagcg cgctgacgct cgccgcgtgc
     2401 ctgggcaacc gcttcgacgc cgggacgctc gcgcgcgtct gcgatcggtc gtcgcgcgcc
     2461 gtgctggccg atctggatgc cgcggttgcg gagggtctgc tggtgccgga ggccaatcag
     2521 gcgttcgcgt tcctgcacga tcgcgtgcag caagccgcct acgcgcgcct gtctgccgac
     2581 gaccgcgcgc ggctgcacct gcagatcggg cgcgcgctcg ccaccgatgc gaccgccacg
     2641 ggccacgacg cgtccgggtt cgatcgcgtc ttcgacgccg cgtcgcacct gaacctgggc
     2701 gcacggctaa tcgacgcacc ggaggagaag ctcgcgctgg caaggttgaa tctcgccgcg
     2761 ggcgacaagg caaggacgtc ggccgcctac cgcagcgcac tcggttacct cacggccggg
     2821 atcgcgctgc tcgatgcgtg ccactggggg tccgaatacg aactcgcgtt cacgctgcac
     2881 ctggaggccg cgcagtgcga gtacctgtgc ggcgatttcg acaccgcgct gcgccagtac
     2941 gccgcactgc tgcagcgcgc ggcgtcggac ctggatcgcg cgcgggtgca ccggctgcgc
     3001 gcgatccagt tcgagaacat ggctcgctac accgacgcgc tcgacgccgt gcgcgcggga
     3061 ctggccgcgc tcggcatcgc gctgccggcg tcggcacgcg ccaggcgttc ggcgctggcg
     3121 cgggaaatcg cgcagatccg cgcacgtctc gccggccgca cgatcgcgtc gctgatcgac
     3181 ctgccgccca tgatcgagcc gtcgatgcgg atggtgatga agctgctgac cgatgcctgg
     3241 tcatcggcat acatccaggg ggacgcgacg ctggcgcggc tgatctcggc catgctggtg
     3301 cggttgtcgc tcgttcacgg caacgcggag gaatcggcat acggctatgt cacccacgcg
     3361 atcaccgccg gcccggtgca aggcgattac gacgaggcgt ggcagtgggg agccctcgca
     3421 ctgcaagtca acgagcgcct ggaggatcgc cgcctgcgcg ccaaggtcta ccagcagttc
     3481 catgcccacg tgaacctgtg gtgccgcccg tttgcagcct gcgtccagta cgcgaaggaa
     3541 gcgtgccgcg cgggcctgga aagcggggat ttcctgtacg ccgcctacgg cgcatccacc
     3601 gagtcatggc cggccttcgc cgccgcacag gatctcgcgg gcttcgtcgc cgacctgcag
     3661 ccgaacctcg cactgctgac gcggctgaag aacgacagct tcgccgacgc gctgcggctg
     3721 atgatcgcct ggtcactcgc actgcagggt gccgccatgg acgcgccgcg ttcgctgtcg
     3781 aatcccgact tcgacgagga ccggtatgtc gagacgtacc gcggcaacgc gttcttctcg
     3841 atgttccacg cgatcgcacg gctcgccgtg tgcttcctgc tcgacgatgc cgctggcgcg
     3901 gccagcgccg cgcgctcggt gcagtccacc gcgcacgagc tgatcggcat gctgtggagc
     3961 gtccagttct ggttctggaa cggcatgcgg ctggccgaag cgtatgccgg cgcggacgcc
     4021 gccgggcgcg ctacggcgct caaggagctg cgcgcggcgc ggcgcacgct cgacgcgctc
     4081 gccgagagct gcccggagaa ctttcgctgc tcggcgctgc tgctggcggc ggaacaggcg
     4141 cgcatcaccg agcgaccctt cgaggcgctc gacctgtacc agcgcgcgat cgcctacgcg
     4201 gaagaggtcg cgcagatcca gcaccgcgcg ctggccagcg aacgctgcgg attgctgtgg
     4261 ctcgaacgcg gcaatcgcga cgtggcggcg cactatctgc gcgccgcgcg cgacggttat
     4321 gcgcagtggg gcgcgcaggc gaaggtgaag cgcctgctcc aacgccatgg cgaactgctt
     4381 ggtgcatcgg agcggatcgc ctcggcggca tcggttccgc cgcgcgcgcc ggagtcgctc
     4441 gacatcgcca gcatcggcaa ggctgcgcac gcgctcgccg ccgacgtcga actcgacacg
     4501 ctgatccgcc ggatgctgcg gatcgccatc gaaagcgccg gcgcgagccg cggcgcgctg
     4561 gtcgaggtgc aggacggcag cgcgcgcgtc gttgccgacg gcagcgccga gtccgacagc
     4621 ggcatcgtgc tgcgcgacgc gccgctcgac gcgaacccgc gcacctgcag cccggcgatc
     4681 gtcaactacg tgttgcgcac gcaggcggcg ctggtgctga gcgaaccgtt gctcgacgag
     4741 cgctttgcgc acgacgaata cctggccggt gcgcggccca agtcggtgct gtgcctgccg
     4801 atcgtccagc agcgcggcat gcgcgcggtg ctctacctgg agaacggcct ggtgcgcgac
     4861 gcgttcggcg ccgagcgcat cgaggtgatc cagatcctgg cctcgcaggc agccagtgcg
     4921 ctgcacagcg cgcgcctgct cgaccgcatg cagcgcgaga tgcatgagcg cgaacgcgcc
     4981 gagcagcagc tgcgcgcgat cgaggccggc acctcgaccg tgaccggcgg cgatttcttc
     5041 cgcgcgctgg tgcgcaatct cgcgctggca ctgcgggtgc gctacgtgtt cgtggccgag
     5101 tgcctgcgcg atgatcccgg gcgcggccgc atcgtgcgat cgcgcgcgtt ctggaacggc
     5161 gacgacttcg gcgacaactt cgagttcgcg gtgccgggaa cgccgtgcca gcacgtgatc
     5221 gagggcgagg tctgccacta tgcggaggac ctgcaggcgc ggttccccga ggacgccgga
     5281 ctggcgcgct gggacgcgca cagctatctc ggcatgcccc tggtcgattc cggcggcacg
     5341 gtgatcggcc acgtggccgt gctggacacc gaaccgatgc cggacgcgac actggcgcgc
     5401 gcagtgctgc gcctgtcggc cggccgcgcc ggcgcggagc tcgagcgcct gaaagcccac
     5461 gagggcctgc agcgcgcgct ggtcaaggtc gaggagttga agaaccggct gcaggaagag
     5521 aacgtctatc tgcggcgcga gctgatcgcc aacgtctcgc acgacctgcg cagcccgctg
     5581 gcatcgctgc gcgggtacct ggacacgctg ctgctcaagg agcagacgct ttcggctgcg
     5641 gagcggcgtt cgttcctgtc gatcgcacag cgacaggccg agcacctgca gacgctgata
     5701 tcggagctgt tcgatctcgc gcggctcgac ttcgacggct accgcatcga cgccgagccg
     5761 gtacacctgg gggaacttgc gcgcgacgta ctgcagaaat tccagctcgc ggcgcagaag
     5821 aaacatgtgc agctcgcact cgcgatcgac gacgacctgg ggttcgtgag cgccgacatc
     5881 ggcctgctgg agcgcacgct ggagaacctg ctggagaacg cgctcgcgca cacgccggcg
     5941 gccggccgcg tcgagctggc ggtacttgcg cacgaggtcg gtgcgctgat acaggtcagc
     6001 gacacgggcc gcggcattcc gccggacgac ctgccgcgaa ttttcgagcg cttctaccgc
     6061 gtcgacaagg cgcgcacgct gaactcaagc gggagcgggc tggggctcgc gatcgtcaag
     6121 cgcatcgtcg aactgcacgg cagccggatc cacgtggaaa gcaacaccgg caggggcacg
     6181 cggttctggt tcacgctgca gaccgccgcg gccgccgcgt cgcagccgac cgtcacaaca
     6241 gaacgacctg cctgatcgcc cggccctcgc gcagccgatc gaatccttcg ttgctctcgt
     6301 ccagcgtcat ggtgccggac agcagccgtc gcaccggcag gcgcccctgc cggtgcaacg
     6361 cgatgtagcg cggcagatcg cgcaacggca cgcaggtgcc gatgtaactg cccttgaccg
     6421 tgcgctcctc cgcgacca
//
LOCUS       DBNHBY010000359        15240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1239909, whole genome shotgun sequence.
ACCESSION   DBNHBY010000359 DBNHBY010000000
VERSION     DBNHBY010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 15240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1239909"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1295)
                     /gene="pmbA"
                     /locus_tag="AC16HB_08890"
                     /pseudo
     CDS             complement(1..1295)
                     /gene="pmbA"
                     /locus_tag="AC16HB_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311612.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
     gene            1334..1864
                     /gene="yjgA"
                     /locus_tag="AC16HB_08895"
     CDS             1334..1864
                     /gene="yjgA"
                     /locus_tag="AC16HB_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR5606945.1"
                     /translation="MKSLPIDPAADEAAQQERPSKSQRKREMAALQDLGVALVRLPAD
                     QLAHIELPENLHEAIVAARRITDHEGRRRQLQYIGRLMRAVDAEPITRALAAATGGSK
                     ATVELMHRCERWRDRLLDDDAALTELLRDHPGIDAQSLRATIRAARREREQGGAPKHM
                     RALYRWLHDTLRTANA"
     gene            1861..2478
                     /gene="mog"
                     /locus_tag="AC16HB_08900"
     CDS             1861..2478
                     /gene="mog"
                     /locus_tag="AC16HB_08900"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HIR5606946.1"
                     /translation="MKAQRSHPDELIVGLVSISDRASAGVYEDKGVPGLRDWLARALA
                     SPWQAHERLIADERAVIEATLKELVDDVGCDLVLTTGGTGPARRDVTPEATLAVGTRE
                     MPGFGEQMRRISLEFVPTAILSRQVAGVRETAAHAALIVNLPGQPKAIQETLEGLRDA
                     AGASRVPGIFAAIPYCIDLIGGPYVETNGDVIRAFRPKSAIRKTA"
     gene            2475..3149
                     /locus_tag="AC16HB_08905"
     CDS             2475..3149
                     /locus_tag="AC16HB_08905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5606947.1"
                     /translation="MSEALQCVELQTRDRPDACVIWLHGLGADGYDFVPVVRELEALG
                     VPPLRYVFPHAPMRPVTINGGYVMRAWYDIFGTELVRREDDVSIRASQRAVEDLIARE
                     VERGIARARIVLAGFSQGAAITLQTGLRQREPLAGMMALSCYLPLAASVAAERAPESA
                     HVPIFMAHGASDPVIPLTRATASRDALLALGYAVQWHEYPMQHSVSAEELGEIAAFLS
                     NTLRAR"
     gene            complement(3171..3869)
                     /locus_tag="AC16HB_08910"
     CDS             complement(3171..3869)
                     /locus_tag="AC16HB_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR5606948.1"
                     /translation="MITVRRSDERGYADHGWLRSYHSFSFADYYDPRHMGFSSLRVIN
                     EDRIAPGAGFGTHGHRDMEIVSYVLDGELAHKDSMGNGSVIRPGDVQRMSAGTGVRHS
                     EFNHAPDRVTHFLQIWIEPDRQGLPPSYEERRFDAIEKRGRLRLIASPDGADGSVTLH
                     QDARLYAALLDRAEAATHVLAAGRGAYVHVVSGALDVNGVALAGGDALLVRDEPELRL
                     TRGREAEVLLFDLR"
     gene            complement(3912..6188)
                     /locus_tag="AC16HB_08915"
     CDS             complement(3912..6188)
                     /locus_tag="AC16HB_08915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR5606949.1"
                     /translation="MSTAGSAALPLASSLLHAERRALPAALLLIAAVSLTLPGASNWV
                     GLWTILTTASAALRWLIARWSEPPGEVGAARVVAALHLADIALWCALLLTVPAAYGVN
                     GIAAAGAAGAALLASISLRHVAGPSNVLMAGWLLAAVVVILSKAAAPLFIVGFPLWLL
                     AVWWSARLPDAALFAAPLEAPAAAPKHEPAPVPPEPAASQADDLYARLPGLVWNVDAR
                     GHVTEVRGDAPWRWGLKIGAYERPHWAEAFDIDPDSRADVERAIARAIAGQSTFDVLN
                     RRRTRLGALVALRSHFVPFAVADGGSGESNASALVLDTIAAPHALIEVEQLRRSRQQY
                     KSLVDASASLIWACDEHFRISFVSRRATREIYRCEPHHLVGRSLSKLLAEGVDQHAAL
                     RGLAGLRAGESLRDVEMVHGAFDGSRVVVALTAAPLTTRDGAFRGAVGTVTDLTIMKQ
                     RESRLAEALQVERTVLDAAGEAIAVVKGGKVLRCNEAFMRLLKAEPAHLARANLAEYF
                     ERSEDWAELAGAAEFARGEDRATVKEVQIKRGARRDTESMWCQVTARMVAPDEFVIVL
                     VDIDQIRRRETDALHVAHHDELTGLPNRRLLATRAQPALAAAAAGRGICAVLALDLDG
                     FKAINDRYGHRMGDVVLREIAHRLQRVVRPQDTVARRGGDEFAVLLPDVGTRLDAERV
                     ASRLLHAVGQPLILTGGREGWVSASIGIALAPQQGIDLERLLQVADTAMYQAKVQGKN
                     RYEFPSEAHEGTRTAAAQ"
     gene            complement(6353..8695)
                     /locus_tag="AC16HB_08920"
     CDS             complement(6353..8695)
                     /locus_tag="AC16HB_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HIR5606950.1"
                     /translation="MTPRPATLFLTLPPEPNVAGELLRICMRLGRVTWVGNLGLALLV
                     VMAMWGSPPALVGLGWWVVLGFVLLARGAYCARQVARSDLPAERAHLNYALFASAEGV
                     LWAAGLIALPAPSPTGMLLQFGLSIAAVVGSLLPYGAIPRAWLASAVPLGFAQFALLL
                     TRDLPMVVPLAFAWAICIGGAAFASLRLQRGVSENLRVRFQAQEAVAQKEQVVAELHR
                     GRDQLRLALEAIDAGIADTNVVTGERFFSARYAEILGYADRGEFMQAHRFSEALHDDD
                     RARVLEARRRHIEEGVPFREEFRLRTARGDHIWVQARGESTRDGDGRATRFVMSIVDV
                     TARREAETKLISSERRYRALVEASPSLIWMCDHDGKLTFVSTRGSRGLYGYEPRHMLG
                     KHVTAFNAPDFGRREFLRRFLPVFHGLPVYDIEATHLSRTGKAIHVTVSALPTLDANG
                     RIESVLGICTDITALKGRERELRVALRNQQVIFDAAGEGIAFVRDGAIESANGALAKM
                     LGVTREWLMGRPVREILARPADWEDIRGVTHAAGRQGEAANHEVMMRAPDGGTGGRSV
                     WCQLTSRTVGDEGEAEAAAKMILVLTDITTLKRREELAWHQANHDELTGLPNRRLLVE
                     NARRLLSVAMRRKRLAALMLLDLDGFKEVNDVFGHAHGDTLLRRVALRLSSVLREYDL
                     VARTGGDEFVVLLPEIDEPQVANHVAEKLIAAAADNIEDTGRSVRIRASVGIALFPAD
                     GQDFDSLMGSADAAMYAAKAAGKNQYRFAAPPADSAPQMH"
     gene            8775..9683
                     /gene="gluQRS"
                     /locus_tag="AC16HB_08925"
     CDS             8775..9683
                     /gene="gluQRS"
                     /locus_tag="AC16HB_08925"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR5606951.1"
                     /translation="MAAVDSTPPPYRGRFAPSPTGALHAGSLVAALASWLDARAHGGT
                     WLVRIEDLDPPRTVLGAMQDILGTLAAFELDSDAPILRQGERGDRYEAALARLQSQGL
                     VYGCACSRRDIEEMAAQTGLPAGVYPGTCRLGTLGRPPRALRVRVPPALIAFEDRACG
                     RYAQQLERDVGDFVVRRADGMWAYQLAVVVDDAHQRITDVVRGADLLDNTPRQIFLQR
                     ALGLPTPRYLHVPVVTNPAGEKLSKQTGAVALDRADALGELERAWRHLGFERIGADSR
                     GAFLARATELWRERWCAPSTITSPLS"
     gene            9691..10179
                     /locus_tag="AC16HB_08930"
     CDS             9691..10179
                     /locus_tag="AC16HB_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5606952.1"
                     /translation="MADDTDIPTLRRILHDNQRIAVVGISAEWHRPSYFVGKYLLEHG
                     YTMIPVNPKYTEVLGQRCYPNLRTAAKDAGPIELVDCFRRAEDIPALADDAIAIGARC
                     LWMQLGVVSQAAAAKARAAGLDVVMDRCVKIEHGRLFGGLNWAGVNTRIVSSKRPKQL
                     PY"
     gene            10190..11488
                     /locus_tag="AC16HB_08935"
     CDS             10190..11488
                     /locus_tag="AC16HB_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR5606953.1"
                     /translation="MDRTFGIETLCLHAGQLPDPVTGSRAQPIYQTTSYVFDSADHAA
                     SLFNLQTFGNVYSRISNPTVAAFEERVAALENGRAALAASSGMAAQLTGILAIVQPGQ
                     HIVASSLLYGGTYAQLGVTLPKMGIETTFVDPDDPENFRRALKPNTRLVYGETLGNPK
                     INVLDIERVAAIAHEAQIPLAIDNTMPSPFLCRPFDFGADIVVHSATKYMGGHGTTIG
                     GVLVERGKFDWGNGKFPDMVLPSKGYHGVKFWETFGDFAYTMKARFEVLRVHGSSLSP
                     ISAWLLLQGLETLHVRMERHVQNARRVAEFLEAHPGVQWVNWPGLRSSPYYELAQKYL
                     RPVDGAPGASSVITFGIKGGAAAGERFIDSLQFLSHLANIGDAKSLVIHPASTTHRQL
                     SEEEQRAAGVLPEMVRLSIGIESIDDILWDLEQALARSAA"
     gene            complement(11492..12727)
                     /locus_tag="AC16HB_08940"
     CDS             complement(11492..12727)
                     /locus_tag="AC16HB_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5606954.1"
                     /translation="MQQDRNSARGFWWPVSAAALLLAITLGTRSSLGLFVGPINSATA
                     LGAAAVGLALALSQLAWGAAQPLWGLAAQRLGTARVVALGGVLAAAGLALVTVASTQG
                     ALIGALLVAGAAGAAAGGAPLLMGAVAQRVAAERRGFALGIISAGGSAGQLVLAFVGA
                     ALIGGAGWQVAMLAFGALALAAVPLGRAFAAQSPAVDRPAPSAPAPAHMSARAALRAP
                     SYWCVTFGFFVCGFHVSFLTTHMPSVIDLCGLPTAFSGLWLAIVGACNIAGSLGAGWL
                     MQRVPMKWMLAALYALRATGVALFLWLPTTPSLLLGFALWMGFTFMATLPATTGLIAK
                     LYGARHVALLFGITMMLHQVGSFFGVWFGGLELELTGGYRWTWVIDMALAAFAALVHP
                     PVREEGIPAPAAARLRQAT"
     gene            complement(12874..>15240)
                     /locus_tag="AC16HB_08945"
     CDS             complement(12874..>15240)
                     /locus_tag="AC16HB_08945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR5606955.1"
                     /translation="LRVIPIRHRGEPHVLAIARDITERRGAEEQLRASEARYRLLFET
                     ESDAILLVDAESLRLVDVNRAAMQMYGFEREELLARTALDLSTDPEQTRAAIQKPAGA
                     VMVPLRWHRHRDGTVFPVEINANRLQLDGRTIVVAAIRDITERKRAEQALRASEEQYR
                     SIFNASADAMVLRDAELRLVDANAAFLVRHGVRREDVIGSADMTFITRDCAQDVEALL
                     RAALAGHGGVVEARTVAQGGTVLDIEVRAVPMQYDCKPHVLSIARDITERRAREAALR
                     ASEEQYRTIFNASADALVLWDANLKRVDVNPAYERIFGWSRDEVIGQGYEHRNLALDY
                     AERRLDLVRRALAGEMCHAELESVRKSGERFRAEIHVIPIQYGGAPHVLAIVRDITER
                     WRAEEQRALLESQLRQAQKMEAIGQLTGGIAHDFNNILASVMGYLVLAEERATDTGDD
                     KSVRYLSEAQASCRRARDLIQQMLTFSRGGRGEPRAVSLTSLVQDALPMLRSVLPSTL
                     AIDVECDPDVPSVWIDELQTYQILLNLAINARDAMNGTGRLRIRVARGAVANLSCTSC
                     RHVLCGPHVELAVTDTGSGIAPELLERIFDPFFTTKEPGKGTGMGLSVVHGIVHEHGG
                     HVVVETAIGAGSTFRVLWPVGRHEASVRAPVKNERRRRAALNGRVLLVDDEPAVLDPM
                     RELLEGWGLDVTAVSSPGEAQATYARDPDAFDLLVTDYAMPLTTGLELAARLRALKSG
                     LPWLLCTGFADEATVARAKAQGADAVLGKPVEPAELRAALERCLSSSG"
BASE COUNT         2346 a         5445 c         5256 g         2193 t
ORIGIN      
        1 ccgcgctcgt agcggtcggc gcccacggcg gcgatgcgtg caaacatgtc cggcacgcgg
       61 cctgcgatcg tgatctcttc gaccgggtag cggattgcgc cgccctcgac ccataagccg
      121 ctcgctccgc gcgaatagtc gccggtgaca tagttgacgc cctgcccgat cagttcggtc
      181 accagcagac cggtgccgag cttgcggatc atcgtcgcaa agtcgtcgcc ggactgcgtc
      241 tgcgtcgagc gcagctcgag gttgtaggcg ccgcccgcgt tgccggtcgt ctttagtccc
      301 agcttgcgcg ccgaatagct cgacaggaag tagccgttca gcacgccgcc ggcgaccacg
      361 tcgcgctgct ggccgcgcac gccttcctcg tcgaacgcgc cggagccgga tgcccgctgc
      421 aggtacggat cctcgtgcac cgaaacgtgc ggggcgaaca gcggcttgcc gagcgcgtcc
      481 acgaggaacg acgccttgcg gtagagcgac ccgccgctgg ccgcctgaac gaagctgccg
      541 agcagcccgc acaccagcgg cgcctcgaac agcaccgggc actggcgcgt gccgagcttg
      601 cgtgccgaca gccgcgccag cgcacgcctt gccgcatagg cgccgatcgc ctcgggtttg
      661 gccagctcct gcggcacgcg cgacgacgaa taccagtcgt cgcgctgcat gccgtccttg
      721 ttcttcgcga tcggcgtgac cgcgatcgag tggcgcgagt acgggaagcc gccgacgaat
      781 ccgcgcgtgt tggcgaggat gaagtggccg gaattggtgt agacgttcgc gccctcggag
      841 ttgacgatct gccggctcac gtcgaacgcc gcctgctcgc agcggcgcgc gagttcgatc
      901 gcggtatcgg tatcgaggtc ccaggggtgg aacagatcga ggtcgcgcgg cgcgcgctcg
      961 agcatgtcct catcgggcag gccggctgcg tcgtcctcgg cggtgaagcg cgcgatgttg
     1021 taggccgctt cgaccgcctg cgcgatcgcc gccggcgcga agtcggaggt cgacgcattg
     1081 ccgcgccgct ggccgatgta gaccgtgatg ccgagacctt tgtcgcgcgt gtgctcgatc
     1141 gtctagacct gcatcttgcg cgtcgacacc gacagccccg tggcctcggt cacctcgcac
     1201 gaagcgtcgg tcccgccgag cctgcgcgcc agcgcgagcg cttcgactgc cagctcacgc
     1261 aggcggtcat cggacagggc aagcagggaa gtcatcggcg gggcttgatt gcgggccgct
     1321 atgatagccg ccgatgaaat cgctcccgat cgaccctgcc gccgacgagg ccgcgcagca
     1381 agagcggccg agcaagtcgc agcgcaagcg cgaaatggct gcgctgcagg acctcggcgt
     1441 ggcgctggtg cggctgcccg ccgaccagtt ggcgcacatc gagttgcccg agaacctgca
     1501 tgaggcgatc gtcgcggcgc gccgcatcac cgatcacgaa gggcgccgcc gccagcttca
     1561 gtacatcggc cggctgatgc gcgcggtcga cgccgaaccg atcacgcgcg cgctcgccgc
     1621 agcgaccggc ggctccaagg ccacggtcga actgatgcac cgctgcgagc gctggcgcga
     1681 ccggctgctc gacgacgacg cggcgctgac cgaactgctg cgcgaccatc cggggatcga
     1741 cgcgcagtcg ctgcgcgcga cgatccgcgc cgcgcggcgc gagcgcgaac aaggcggcgc
     1801 gccgaagcac atgcgcgcgc tttaccgctg gctgcacgac acattgcgga ccgccaacgc
     1861 atgaaagcac aacgcagtca tcccgacgaa ctgatcgtcg gactggtgtc gatctccgac
     1921 cgcgcctccg ccggcgtgta cgaggacaag ggcgtgcccg ggctgcgcga ctggctggcg
     1981 cgcgccctcg cctcgccgtg gcaggcgcac gagcggctga tcgcggacga gcgtgcggtg
     2041 atcgaggcca cgctgaagga actggtcgac gatgtcggct gcgacctcgt gctgaccacg
     2101 ggcggcaccg gcccggcgcg gcgcgacgtg acgcccgagg cgacgctcgc cgtgggcacc
     2161 cgggaaatgc cgggcttcgg cgagcagatg cggcgcattt cgctcgagtt cgtgccgacc
     2221 gcgatcctgt cgcgccaggt cgccggggtc cgcgaaaccg ccgcgcacgc ggcgctgatc
     2281 gtcaacctgc cgggtcagcc gaaggcgatc caggagacgc tcgaagggct gcgcgacgca
     2341 gccggagcat ccagggtgcc cggcatcttt gcggcgattc cgtattgcat tgatctcatc
     2401 ggcgggccgt acgtcgaaac gaacggcgac gtgattcggg cgttccggcc gaagagcgca
     2461 attcggaaga ccgcatgagc gaggcactgc agtgcgtcga actgcagacg cgggaccgcc
     2521 ccgacgcctg cgtgatctgg ctgcacggcc tcggcgcgga cggctatgac ttcgttccgg
     2581 tcgtgcgcga actggaggcg ctcggcgtac cgccgctgcg ctacgtgttc ccgcacgcgc
     2641 cgatgcgccc ggtcacgatc aatggtggct acgtgatgcg cgcgtggtac gacatcttcg
     2701 gcaccgaact cgtgcggcgc gaggacgatg tcagcatccg cgcgtcgcag cgcgccgtcg
     2761 aggacctgat cgcgcgggag gtcgagcggg gtatcgcgcg cgcacggatc gtgctggccg
     2821 ggttctcgca gggtgcggcg atcacgctgc aaaccgggtt gcgccagcgc gaaccgctgg
     2881 cgggcatgat ggcgctgtcg tgctacctgc cgctcgccgc gagcgtggcg gccgaacgcg
     2941 cgccggaaag cgcgcatgtg ccgatcttca tggcacacgg tgcgagcgat ccggtgatcc
     3001 cgctcacgcg tgccaccgcg tcgcgcgacg cgctgctggc gctcggctac gcggtgcagt
     3061 ggcacgagta tccgatgcag cactccgtca gcgcggagga gctgggcgag atcgccgcct
     3121 ttctttcgaa cacgctgcgc gcgcgctgag ccgcttcgaa cggcagcgct tcagcgcagg
     3181 tcgaacagca gcacctccgc ctcgcggccg cgcgtgagcc gcagttccgg ttcgtcgcgc
     3241 accagcagtg cgtcgccgcc cgccagcgcg actccgttca catcgagcgc gccacttacc
     3301 acgtgcacgt atgcgccgcg cccggccgcg agcacgtgcg tcgccgcttc ggccctgtcg
     3361 agcagcgccg cgtacagccg cgcgtcctgg tgcagcgtga ccgaaccgtc ggcgccgtcg
     3421 ggcgatgcga tcagccgcag ccggccgcgc ttctcaatgg cgtcgaagcg cctttcctcg
     3481 tagctcggcg gcaggccctg acggtccggc tcgatccaga tctgcaggaa gtgcgtgacg
     3541 cggtccggcg catggttgaa ctccgaatgc ctgacgccgg tgcccgcgct catccgctgc
     3601 acgtcgccgg gacggatcac cgagccgttg cccatgctgt ccttgtgcgc gagctcgccg
     3661 tcgagcacgt agctgacgat ctccatgtcg cgatggccgt gcgtgccgaa gccggcgccc
     3721 ggcgcgatgc ggtcctcgtt gatcacgcgc agcgaagaga agcccatgtg gcgcggatcg
     3781 tagtagtcgg cgaacgagaa gctgtgatac gagcgcaacc agccgtggtc ggcatagcca
     3841 cgctcgtcgg agcggcgtac ggtgatcatg ggagtcgggc ctccgcagga atggcgcccg
     3901 gcgagattac gtcactgtgc cgctgccgtg cgcgtgcctt cgtgcgcttc ggacgggaac
     3961 tcgtagcggt tcttgccttg caccttggcc tgatacatcg cggtatcggc gacctgcagg
     4021 agccgctcca gatcgatgcc ctgctgcggt gccagcgcga tgccgatgct ggccgacacc
     4081 cagccctcgc gcccgcccgt caggatcagc ggctggccga ccgcgtgcag caggcgcgag
     4141 gcgacgcgtt cggcgtccag ccgcgtgcct acgtcgggca gcagcaccgc gaattcgtca
     4201 ccgccacggc gcgcgacggt gtcctgcggc cgcaccacgc gttgcagccg gtgcgcgatc
     4261 tcgcgcagca cgacgtcgcc catccggtgc ccgtagcgat cgttgatcgc cttgaagccg
     4321 tcgaggtcga gcgccagcac cgcgcagata ccgcggccgg ccgccgcagc cgcgagcgcc
     4381 ggctgcgcgc gcgtggcaag caggcgccgg ttcggcaacc cggtcagttc gtcgtgatgc
     4441 gcgacgtgca gcgcgtcggt ctcgcggcgg cggatctgat cgatgtcgac cagcacgatc
     4501 acgaactcgt cgggcgcaac catgcgcgcg gtgacctggc accacatcga ttccgtgtcg
     4561 cgccgcgcgc cgcgcttgat ctgaacttcc ttcaccgtcg cgcggtcctc gccgcgcgcg
     4621 aactcggccg cgccggccag ttcggcccag tcctcggagc gctcgaagta ctcggcgagg
     4681 ttcgcgcgcg ccaggtgcgc cggctcggcc ttcagcaggc gcatgaacgc ctcgttgcag
     4741 cgaagcacct tgccgccctt gaccacggcg atcgcctcgc ccgccgcgtc gagcacggtg
     4801 cgctcgacct gcaatgcctc ggcgagccgc gattcgcgct gcttcatgat cgtcagatcc
     4861 gtcaccgtgc ccaccgcgcc gcggaacgcg ccgtcgcgcg tggtgagcgg tgcggcggtc
     4921 agcgcgacga ccacgcggct gccgtcgaac gcgccgtgca ccatctccac gtcgcgcagc
     4981 gattcgcccg cgcgcaggcc ggcgagcccg cgcagcgccg cgtgctggtc cacgccctcg
     5041 gcgagcagct tcgacagaga tcggcccacg aggtgatgcg gctcgcagcg gtagatttcg
     5101 cgcgtggcgc ggcggctcac gaacgagatg cggaagtgct cgtcgcaggc ccagatgagg
     5161 ctcgccgatg cgtcgaccag cgacttgtac tgctggcggc tgcggcgcaa ctgctcgact
     5221 tcgatcagcg cgtgcggcgc ggcgatcgta tcgagcacca gcgccgacgc gttcgattcg
     5281 ccgctgccgc catcggccac ggcgaacgga acgaagtggc tgcgcagcgc gaccagggcg
     5341 cccagccgcg tgcggcggcg gttcagcacg tcgaaggtcg actgccccgc gatcgcgcgc
     5401 gcgattgcac gctccacgtc ggcgcgactg tccgggtcga tgtcgaacgc ctcggcccag
     5461 tgcggtcgct cgtaggcgcc gatcttcagg ccccagcgcc acggcgcgtc gccgcgcacc
     5521 tcggtcacgt gcccgcgcgc atcgacgttc cataccaacc ccggcaggcg cgcatacagg
     5581 tcgtccgcct ggctcgccgc gggctccggg ggtaccggcg cgggctcgtg tttcggcgcg
     5641 gcggcgggcg cttccagcgg tgctgcgaac agcgccgcat ccggcaggcg cgccgaccac
     5701 cagaccgcga gcagccagag cggaaagccg acgatgaaca gcggcgccgc ggccttgctc
     5761 aggatgacca ccacggcggc cagcagccag cctgccatca ggacgttgct tgggccggcg
     5821 acgtgccgca gcgagatgct cgccagcaac gccgcacctg ccgcgccggc cgcggcgatg
     5881 ccattgacgc cgtacgcggc cgggaccgtc agcagcagcg cgcaccacag cgcaatgtcg
     5941 gccaggtgca gcgcggcgac cacgcgcgcg gcgccgacct cgccgggggg ctcgctccag
     6001 cgggcgatca gccagcgcag cgcggcggat gcggtcgtca ggatcgtcca caggccgacc
     6061 cagttcgaag cgccgggcag agtcaggctg acggcggcga tcagcagcag cgcggcaggc
     6121 agcgcgcggc gctcggcgtg cagcaacgaa gacgcaagcg gcagcgcggc ggacccggca
     6181 gtgctcaaga ccggcggctc cgcaccgtgg gcggacggcg gatcgtcaac gcgcgcgccg
     6241 cccggccggc tcgatcgttc cgcacgtacc ccatgatgtt gtggtcccac tgtccgaagc
     6301 aacgagccat agtacttgtc accccgcgcg cgggcaaggc gcgcgggcca cgtcagtgca
     6361 tctgcggcgc ggaatccgcc ggtggcgccg cgaagcggta ctggttcttg ccggccgcct
     6421 tggcggcgta catcgcggcg tcggcgcttc ccatcaggct gtcgaaatcc tggccatcgg
     6481 ccgggaagag agcgatgccg acgctggcgc ggatgcggac cgagcgtccg gtgtcctcga
     6541 tgttgtcggc ggcggcggca atcagcttct cggccacgtg gttggccacc tgcggctcgt
     6601 cgatctccgg cagcagcacg acgaactcgt cgccgccggt gcgcgcgacc agatcgtatt
     6661 cccgcaaaac gctcgacagc cgcagcgcga cccggcgcag caacgtgtcg ccgtgcgcgt
     6721 ggccgaacac gtcgttcact tccttgaacc cgtccagatc gagcagcatc agcgcggcca
     6781 ggcgcttgcg ccgcatcgcc accgacagca gccgccgcgc gttctccacc agcaggcgcc
     6841 ggttcggcag gcccgtgagc tcatcgtggt tggcctggtg ccaggccagt tcttcacgcc
     6901 gcttcagcgt cgtgatgtcg gtcagcacca ggatcatctt ggcggccgcc tcggcctcgc
     6961 cctcgtcgcc gaccgtgcgc gacgtgagct ggcaccacac gctgcggccg ccggtgcccc
     7021 cgtcgggcgc acgcatcatc acctcatggt tggccgcctc gccctgccgg ccggccgcgt
     7081 gcgtgacccc gcggatgtcc tcccagtcgg cgggacgggc caggatctcg cgcaccggtc
     7141 ggcccatcag ccattcgcgg gtgaccccga gcatcttggc gagcgcgccg ttggcgctct
     7201 cgatcgcgcc gtcgcgaacg aacgcgatgc cctcgcccgc ggcatcgaag atcacctgct
     7261 ggttgcgcag cgcgacgcgc agttcgcgct cgcggccctt cagcgcggtg atgtcggtgc
     7321 agatgccgag caccgattcg atccggccgt tggcgtcgag cgtcggcagc gcgctgacgg
     7381 tgacgtggat cgccttgccc gtgcgcgaca gatgcgtggc ctcgatgtcg taaaccggca
     7441 gtccgtggaa caccggcagg aatcggcgca ggaactcgcg ccggccgaag tcgggcgcgt
     7501 tgaaggcggt cacgtgcttg ccgagcatgt ggcgcggctc gtagccatac agcccgcggc
     7561 tgccgcgcgt ggacacgaag gtcagtttgc cgtcgtggtc gcacatccag atcagcgatg
     7621 gcgatgcctc gaccagcgcg cgatagcgac gctcgctcga gatcagcttg gtctcggctt
     7681 cgcggcgcgc ggtgacgtcg acgatcgaca tcacgaagcg cgtcgcgcgc ccgtcgccgt
     7741 cgcgcgtcga ttcgccgcgt gcctgcaccc agatatggtc gccgcgcgcc gtgcgcaggc
     7801 ggaattcctc gcggaacggc acgccttcct cgatgtggcg ccggcgcgcc tcgagcacgc
     7861 gcgcgcgatc gtcgtcgtgc agcgcttccg agaagcgatg cgcctgcatg aactcgccgc
     7921 ggtcggcata gccgaggatt tccgcgtagc gcgccgagaa gaagcgttcg ccggtcacca
     7981 cgttggtgtc ggcaatgccg gcgtcgatcg cctccagcgc cagccgtagc tgatcgcggc
     8041 cccgatgcag ctcggccacc acctgctcct tctgtgcgac cgcctcctgc gcctggaagc
     8101 gcacgcgcag gttctcgctg acgccgcgct gcagccgcag cgacgcgaag gccgcaccgc
     8161 cgatgcagat tgcccacgcg aacgccagcg gcaccaccat cggcagatcg cgcgtgagca
     8221 gcagcgcgaa ctgagcgaag ccgagcggca ccgcgctcgc gagccaggcg cgcgggatcg
     8281 cgccataggg cagcagcgac ccgaccaccg cggcaatcga caggccgaac tgcagcaaca
     8341 tgccggtcgg cgaaggggca ggcagggcga tcagcccggc ggcccacagc acgccttcgg
     8401 cgctggcgaa gagcgcgtaa ttcagatgcg cccgttccgc cggcaagtcg ctgcgggcga
     8461 cctggcgcgc gcaatacgcg ccacgcgcaa gcagcacgaa cccgagcacg acccaccagc
     8521 cgagtcccac cagcgctggc ggactgcccc acatcgccat cacgaccagc agggcgaggc
     8581 ccaggttgcc gacccaggtg acgcggccca gccgcatgca gatgcgcagc agttcgccag
     8641 ccacgttcgg ctcgggcggc agcgtcagaa acagcgtggc gggtcggggc gtcacgggtc
     8701 gggtcgttac gggtcggccg ttggttcggc gctattgtcg ccgcaaccac gcgcaaacac
     8761 gagttccgcg aagcatggcc gcagtcgatt ccacaccgcc gccgtaccgc gggcgattcg
     8821 cgccctcgcc cacgggcgcg ctgcacgccg ggtcgctggt cgcggcgctg gcgagctggc
     8881 tcgatgcccg cgcgcacggc ggcacctggc tggtgcggat cgaggacctc gatccgccgc
     8941 gcaccgtgct aggcgcgatg caggacatcc tcggcacgct cgccgccttc gagctggaca
     9001 gcgacgcgcc catcctccgg caaggcgagc gcggcgatcg ttacgaggca gcgctggcgc
     9061 ggctgcaatc gcaggggctg gtctacggct gcgcctgctc gcgccgcgac atcgaggaga
     9121 tggccgcaca gaccgggctg ccggccggcg tctaccccgg cacatgccgc cttggcacgc
     9181 tcggccgtcc gccgcgcgcc ctgcgcgtcc gcgtgccacc cgcattgatc gccttcgagg
     9241 atcgcgcgtg cggccgctat gcgcagcaac tcgagcgcga cgtcggcgat ttcgtcgtgc
     9301 gccgtgccga cggaatgtgg gcgtaccagc tcgcggtcgt cgtcgacgac gcgcaccagc
     9361 gcatcaccga cgtggtgcgc ggcgcggacc tgctcgacaa cacgccgcgg cagatcttcc
     9421 tgcagcgcgc gctcgggctg ccgacgccgc gctacctgca tgtgccggtc gtcaccaacc
     9481 ccgcgggcga gaagctgtcg aagcagaccg gcgcggtcgc gctcgaccgc gccgacgcac
     9541 tcggcgaact cgagcgcgcg tggcggcacc tcggtttcga acgcatcggc gccgattcgc
     9601 gcggcgcctt cctcgcgcgg gcgaccgagc tttggcgcga gcgctggtgc gcaccttcta
     9661 caatcacttc accgctcagt taggtgcttc atggccgacg acaccgacat ccccacgcta
     9721 cggcgcatcc tgcacgacaa ccagcgcatt gccgtcgtcg gcatcagcgc cgaatggcac
     9781 cggccgagct atttcgtcgg caagtacctg ctcgagcacg gctacacgat gatcccggtg
     9841 aacccgaagt acaccgaggt gctcggccag cgctgctatc cgaacctgag gaccgcggcc
     9901 aaggacgcgg ggccgatcga gctcgtcgac tgctttcgcc gcgccgagga cattcccgcg
     9961 ctcgccgacg atgcgatcgc gatcggcgcc aggtgcctgt ggatgcagct tggcgtggtc
    10021 agccaggccg ccgcggcgaa ggcgcgcgcg gccgggctgg acgtggtcat ggaccgctgc
    10081 gtgaagatcg agcacggacg actgttcggc gggctgaact gggccggcgt gaacacgcgg
    10141 atcgtttcga gcaagcggcc aaagcagctt ccttactgaa cgcgacgaca tggacaggac
    10201 cttcggcatc gaaaccctct gcctgcacgc cggccagctg cccgacccgg tcaccggcag
    10261 ccgcgcgcag ccgatctacc agacgacgag ctacgtattc gactcggccg atcacgcggc
    10321 gagcctgttc aacctgcaga ccttcggcaa tgtctattcg cgcatcagca atccgacggt
    10381 cgcagcgttc gaggagcgcg tggcggcgct ggaaaacggc cgtgcagcgc tcgcggcgtc
    10441 gagcggcatg gccgcgcagc tcaccgggat cctggcgatc gtgcaaccgg gccagcacat
    10501 cgtcgcgtcg agtctgttgt acggcggcac gtacgcgcag ctcggggtca cgctgccgaa
    10561 aatggggatc gagaccacgt tcgtcgatcc cgacgatccg gagaacttcc gccgcgcgct
    10621 gaagccgaac acgcggctcg tctacggcga gacgctcggc aatccgaaga tcaacgtgct
    10681 cgacatcgag cgcgtcgccg cgatcgcgca cgaggcgcag attccgctgg cgatcgacaa
    10741 cacgatgccg tcgccgttcc tgtgccggcc gttcgacttc ggcgccgaca tcgtcgtgca
    10801 ttcggcgacc aagtacatgg gcggccacgg cacgacgatc ggcggcgtgc tggtcgagcg
    10861 cggcaagttc gactggggca acggcaagtt tcccgatatg gttctgccgt cgaagggcta
    10921 ccacggcgtg aagttctggg aaaccttcgg cgacttcgcc tacacgatga aggcgcgctt
    10981 cgaggtgctg cgcgtgcacg gctcgtcgtt gtcgccgatc tcggcctggc tgctgctgca
    11041 ggggttggag acgctgcacg tgcggatgga gcgccacgtg cagaacgcgc gccgcgtggc
    11101 cgagttcctc gaagcgcacc ctggcgtgca gtgggtcaac tggccggggc tccgatcgag
    11161 cccgtactac gaactggcgc agaagtacct gcggccggtc gacggcgcgc ccggtgcgtc
    11221 gagcgtgata actttcggca tcaagggggg tgccgccgcc ggcgagcgat tcatcgacag
    11281 cctgcagttc ctgtcacatc tggcgaacat cggcgatgcg aagtcgctgg tgatccatcc
    11341 ggcgtcgacc acccatcggc agctgtccga ggaggagcaa cgcgccgccg gcgtgctgcc
    11401 cgagatggtg cggctgtcga tcggcatcga gtcgatcgac gacatcctgt gggatcttga
    11461 acaggcgctg gccaggtccg cggcgtgaga atcaggtcgc ctgccgcagg cgcgccgcgg
    11521 caggcgcagg gatgccctct tcgcgcaccg gcggatggac cagcgcggcg aatgcggcca
    11581 gcgccatgtc gatgacccag gtccagcgat agccgccggt cagctcgagt tcgaggccgc
    11641 cgaaccacac cccgaagaag ctgccgacct ggtgcagcat catcgtgatg ccgaaaagca
    11701 gcgcgacatg gcgcgcgccg tagagcttcg cgatcaggcc cgtcgtcgct ggcagcgtgg
    11761 ccatgaaggt gaagcccatc cacagcgcga agccgagcag caggctcggg gtggtcggca
    11821 gccagagaaa cagcgccacg ccggtcgcgc gcagcgcata cagcgcggca agcatccatt
    11881 tcatcgggac ccgctgcatc agccacccgg cgccgaggct gccggcgatg ttgcaggcgc
    11941 cgacgatcgc gagccacaga ccggagaacg cggtcggcag cccgcacagg tcgatcacgc
    12001 tcggcatgtg cgtggtcagg aacgacacgt ggaagccgca cacgaagaag ccgaaggtca
    12061 cgcaccagta cgacggcgcg cgcagcgccg cgcgcgccga catgtgcgcg ggcgcgggcg
    12121 cggacggggc gggccgatcg accgcgggcg actgcgccgc gaacgcgcgc cccagcggaa
    12181 ccgcggccag cgccagcgcg ccgaacgcca gcatcgcgac ctgccagccg gcaccgccga
    12241 tcagcgcggc gccgacaaag gcaagcacga gctggcccgc cgagccgccg gcgctgatga
    12301 tcccgagcgc gaacccgcgc cgctccgccg ccacgcgctg cgcgaccgca cccatcagca
    12361 gcggtgcgcc gccggccgcg gcgccggctg caccggcgac aagcagtgcg ccgatcaggg
    12421 cgccctgcgt cgatgccacc gtaaccagtg ccaggccggc tgctgcgagc acgcccccga
    12481 gcgcgaccac gcgcgccgtg ccgagacgct gcgcggccag cccccacagc ggctgcgccg
    12541 cgccccacgc gagctggctc agcgccagcg ccagcccgac cgccgcggcg ccgagcgcgg
    12601 tcgcgctgtt gatcgggccg acgaagagcc cgagcgaaga gcgcgtgccg agcgtgatcg
    12661 ccaacagcag cgcggccgcg gaaaccggcc accagaatcc gcgcgcggaa tttcgatctt
    12721 gctgcaccgg ccgcctttcg ttggtgggtc gtcgccgctg cacacgatgc gcagccacgg
    12781 cccagcaagg taggcggtcg cggtaatcgg ggaatttcga tcggcgcgcg ctttgtagcg
    12841 cgcggtttca cgcgcgtaac cgcgcgtccg tgatcagccg ctcgaactta ggcagcgttc
    12901 aagcgcggcg cgcaactcgg ccggttcgac cggtttgccg agcactgcat ccgcgccctg
    12961 cgcctttgca cgcgccaccg tggcctcgtc ggcgaacccg gtgcacagca gccagggcag
    13021 gccggatttc aacgcgcgca gacgcgcggc gagttccagc cccgtcgtga gcggcatcgc
    13081 gtagtcggtg accagcagat cgaaggcgtc gggatcgcgc gcgtacgtgg cctgcgcctc
    13141 gcccggcgac gagacagcag tcacgtcgag cccccagcct tcgagcaact cgcgcatcgg
    13201 gtccagtacc gcgggctcgt cgtcgaccag cagcacgcgc ccgttgagcg cggcgcggcg
    13261 gcgccgctcg ttcttgaccg gcgctcgcac gctcgcctcg tggcggccga ccggccacag
    13321 cacgcggaag gtcgatccgg ccccgatcgc cgtctcgacc acgacatggc cgccatgctc
    13381 gtgcacgatg ccatgcacga ccgacaatcc cattccggtg cccttgcccg gctccttggt
    13441 cgtgaagaag gggtcgaaga tgcgttcgag caactccggc gcgatgccgc tgcccgtatc
    13501 ggtcaccgcg agttcgacgt gcggaccgca cagcacatga cggcagctgg tgcaggaaag
    13561 gttggccacc gcgccgcgcg ccacgcggat gcgcagccgg ccggtgccgt tcatggcatc
    13621 gcgggcgttg atcgcgaggt tcagcaggat ctgataagtc tgcagctcgt cgatccacac
    13681 gctcggcacg tccgggtcgc actcgacatc gatggcaagc gtggacggca gcaccgaccg
    13741 cagcatgggg agcgcatcct ggaccagcga ggtcagcgat actgcgcgcg gttcgccgcg
    13801 cccgccgcgc gagaacgtca gcatctgctg aatcaggtcg cgcgcgcgcc ggcacgacgc
    13861 ctgcgcctcg gatagatagc gcacggactt gtcgtcgccg gtgtcggtgg cacgttcctc
    13921 ggcgagcaca aggtagccca tgacgctcgc caggatgttg ttgaagtcgt gcgcaatgcc
    13981 accggtcaac tggccgatcg cctccatctt ctgtgcctgc cgcagctgcg actcgagcag
    14041 cgcgcgctgc tcctcggcac gccagcgctc ggtgatgtcg cgcacgatcg ccagcacgtg
    14101 cggcgcgccc ccgtactgga tcgggatgac gtgaatctcg gcgcggaacc tttctccgct
    14161 cttgcgcacc gattcgagct cggcgtggca catctcgccg gcgagcgcgc gccgcacgag
    14221 gtcgagccgg cgctccgcat agtcgagcgc caggttgcgg tgctcgtagc cctggccgat
    14281 tacctcgtcg cgcgaccatc cgaagatccg ctcgtaggcc gggttcacgt cgacgcgctt
    14341 gagattcgcg tcccacagca ccagggcgtc ggccgaggcg ttgaagatcg tccggtactg
    14401 ttcctcgctg gcgcgcagcg ctgcctcgcg cgcacgccgc tcggtgatgt cgcgcgcgat
    14461 cgacagcacg tgcggcttgc agtcgtactg catcggcacc gcacgcacct cgatgtcgag
    14521 cacggtgccg ccctgcgcga cggtccgcgc ctccaccaca ccgccgtgcc cggcgagcgc
    14581 ggcgcgcagc agcgcctcga cgtcctgcgc gcagtcccgg gtgatgaacg tcatgtcggc
    14641 actgccgatc acatcctcgc gccgcacgcc atggcgcacg aggaacgccg cgttggcatc
    14701 caccagccgc agctcggcgt cgcgcagcac catcgcgtcg gccgacgcat tgaagatcga
    14761 gcggtactgc tcctcgctgg cgcgcagcgc ctgctcggcg cgcttgcgtt cggtgatgtc
    14821 tcgaatcgcg gccacgacga tcgtgcgtcc atcgagctgc aggcggttgg cattgatctc
    14881 gaccgggaac acggtgccgt cgcggtgccg gtgccaacgc agcggcacca tgaccgctcc
    14941 ggccggcttc tggatcgccg cgcgcgtctg ctccgggtcg gtcgacagat cgagcgccgt
    15001 gcgcgccagc agctcttcgc gctcgaagcc gtacatctgc atcgcggcac ggttgacgtc
    15061 gaccaggcgc agcgactcag cgtcgaccag caggatcgcg tcggactcgg tctcgaacag
    15121 cagccggtag cgcgcttcgc tcgcgcgcag ctgctcctcc gcaccccgcc gttcggtgat
    15181 gtcgcgcgcg attgcgagca cgtgcggctc gccgcggtgg cggatcggaa tcacccgcaa
//
LOCUS       DBNHBY010000360         5318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_21205, whole genome shotgun sequence.
ACCESSION   DBNHBY010000360 DBNHBY010000000
VERSION     DBNHBY010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_21205"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="AC16HB_08950"
     CDS             <1..803
                     /locus_tag="AC16HB_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5606956.1"
                     /translation="GGDVNTRGIKVGRVEDYALVGDKVNRVRVKIRVDRRTPVRVNTV
                     AGITRNFVTGIAQITLITPEPPGPPLETTPDDEPYPVIAEGRNDLEEIAGRVNELSDM
                     ASEVLNKLNRALSAENRLRLGQTLYNLSQLTEALNARMGTFDATLKQVGAAANSFGAA
                     SERIAMSADRATAHLDETLTDAQRTLAEAQRTAVDAGQAVAAIQTQMAAITRRIDHTA
                     VGIDDQLAATVVELRSSIDAVTRALERLQDPRTALLGPGKAQLGPGEK"
     gene            800..1420
                     /locus_tag="AC16HB_08955"
     CDS             800..1420
                     /locus_tag="AC16HB_08955"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIR5606957.1"
                     /translation="MRALLAAIALLLAGCVTVDIGGESTAQTQYVLGDAGAAPAPRAA
                     PVAESLLIQSAAGDPLADTLSIAYARRDGERALYQLATWTDRPTRRIPQLLQRRLEAR
                     GSFRAVAFLGQPLHADWLVAIAIDDIHHEVVAEPGTARLLVRAALFDRHKRTQVAQRA
                     FAADVPVAEAKSAAAARAMSQAVAQVLDALVPWLEQEVERARSAGS"
     gene            1425..1814
                     /locus_tag="AC16HB_08960"
     CDS             1425..1814
                     /locus_tag="AC16HB_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HIR5606958.1"
                     /translation="MRDPDRPSSARNVLGGPPASCCMRPLTGFFRDGYCHTGPQDVGS
                     HTVCVQMTAEFLDFSVARGNDLVTPHPESGFPGLAPGDRWCVCAARWLEAYEAGAAAP
                     VVLAATQEKALDIVPLAALRAHALDLH"
     gene            1822..2814
                     /locus_tag="AC16HB_08965"
     CDS             1822..2814
                     /locus_tag="AC16HB_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5606959.1"
                     /translation="MRARAMLLHAAGRPLAEGTIDVGDPGPGEVRLRVSTCGVCRTDL
                     HIVDGELTQPKLPLVPGHEIVGRIEAIGAGVTRLGVGDRVGVPWLGGTCGHCRYCRER
                     RENLCDTPRFTGYTRDGGYAERSLVDAGYCFALPDTLDDAHAAPLLCAGLIGYRALRF
                     AGDARVLGIYGLGAAAHLLAQVAVWQGRRVFAFTRAGDADGQRFARALGCAWAGASDE
                     LPPEPLDAALIFAPVGALVPTALRATRKGAVVVCAGIHMSAIPSFDYELLWGERTIRS
                     VANLTRADGDEFLALAAQVPVRTHIELFDLARANEAIDAVRAGRVHGAAVLRCS"
     gene            2805..3248
                     /locus_tag="AC16HB_08970"
     CDS             2805..3248
                     /locus_tag="AC16HB_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR5606960.1"
                     /translation="MLVKPDAPRVVLDTNVLLDLWVFDDPGVGSLRAALGAGAYVALR
                     SRVTDTEVADVLARPQFALSIERQRALLNRWRECARPVEDIRRAPWQCDDPHDQPFLD
                     LAFTARAQGLVTKDKALLRLARKARSTGLRIVAPLRFAVAMPACT"
     gene            3329..3829
                     /locus_tag="AC16HB_08975"
     CDS             3329..3829
                     /locus_tag="AC16HB_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HIR5606961.1"
                     /translation="MRRRLYFVLPDLGSAIQTANDLLLSRVEDRHMHFLGRRGMSLGE
                     LHEAGFMQKSDFRHSLFLGTVLGAVGGLVVGVILKLTGIGDIGWLIIATLIGGLFGGW
                     TSTLIGISTPNHTLKPFAADIEAGRILLMVDVPPSRIDEVKALVAKRHPEVADRGVEP
                     LMPAFP"
     gene            complement(3833..4873)
                     /locus_tag="AC16HB_08980"
     CDS             complement(3833..4873)
                     /locus_tag="AC16HB_08980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5606962.1"
                     /translation="MIVASNSRISLRRALPDDRMRIYHWIARNDLAQTMTGNAPLFEP
                     RVPTFDEFRARYAEHFFNGLRPLAGRALLISNGAEDVGFVSYRDINLLRDGVEIDAWL
                     AARRFQGRGYGSDALALACEWLQTEYGVNRFVLRTSRRNVHALRAGRRAGFRQGEGDP
                     REILKELRLGPGEYADETLLLRSLPPRPAALTPEGARTYVFFDSEFTNLREPTLISVG
                     AVATDSTAFYCELQDWPADQASEFVRDVVLPLLDGDAVPLMMARDAFIAWLADRSRDQ
                     PVTIVCDSGYDRQSLTWLLGTEDLPDNVGWLHVPFAYEHLDEVVRALGLRRHHALDDA
                     RGLRHGLLAPPA"
     gene            4954..>5318
                     /locus_tag="AC16HB_08985"
     CDS             4954..>5318
                     /locus_tag="AC16HB_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5606963.1"
                     /translation="MPHHALPPRALADLIGPSRLPNVGTTIFTVMSALAARHGAVNLG
                     QGFPDFDCDPKLQQYFTDAMAAGHNQYAPMAGVAALRQALSVKHEQLYRHRYDPDAEI
                     TVTAGATQAIMATVLALVRP"
BASE COUNT          772 a         1889 c         1879 g          778 t
ORIGIN      
        1 tgggcggcga cgtcaacacg cgcggcatca aggtcggccg cgtcgaggat tacgcgctgg
       61 tcggcgacaa ggtcaaccgc gtgcgcgtga agatccgcgt cgaccgcagg acgccggtgc
      121 gcgtgaatac ggtggccggc atcacgcgca acttcgtgac cggcattgcg cagatcaccc
      181 tgatcacgcc cgagccaccc ggcccgccgc tcgagaccac gccggacgac gagccgtatc
      241 cggtgatcgc cgaagggcgc aacgacctgg aggagatcgc cggccgcgtg aacgaactct
      301 ccgacatggc ctccgaggtg ctgaacaagc tgaaccgcgc gctgtcggcg gagaatcgcc
      361 tgcgcctcgg acagacgctg tacaacctgt cgcagctgac cgaggcgctc aacgcgcgca
      421 tgggcacatt cgacgcgacg ctgaaacagg tcggggccgc cgcgaactcc ttcggcgccg
      481 ccagcgagcg catcgccatg tcggccgatc gcgccactgc gcatctggac gaaacgctga
      541 ccgacgcgca gcgcacgctg gccgaggcgc agcgcaccgc cgtcgatgcg ggacaggctg
      601 tcgcggccat ccagacccag atggccgcga tcacgcgccg catcgaccac accgcggtcg
      661 gcatcgacga tcagctcgcc gccaccgtgg tcgaactgcg ctcgagcatc gacgcggtca
      721 cccgcgcgct cgagcggctg caggacccgc gcaccgcgct gctcggtccg ggcaaggccc
      781 agctcggccc gggggagaaa tgagagcact gctggctgcg attgcgttgt tgctcgccgg
      841 ctgcgtgacg gtcgacatcg gcggcgagag caccgcgcag acgcagtacg tgctcggcga
      901 tgccggcgcc gcgccagcgc cgcgcgcggc gcccgtcgcg gagtcgctcc tgatccagag
      961 cgcggccggt gatccgctcg ccgacaccct gtcgatcgcc tacgcgcgcc gcgacggcga
     1021 acgcgcgctg tatcagctcg ccacctggac cgaccggccg acgcgccgca ttccgcagtt
     1081 gctgcagcgg cggctggagg cgcgcggcag ttttcgcgcg gtggcgttcc tcggtcagcc
     1141 gctgcacgcg gactggctgg tcgcgattgc gatcgacgac atacaccatg aggtcgtggc
     1201 cgaaccgggg acggcgcggc tgctcgtgcg tgccgcgctc ttcgaccggc acaaacgaac
     1261 acaggtggcg caacgcgcgt tcgcggccga cgtgccggtc gcggaagcga aatccgccgc
     1321 tgccgcgcgg gcgatgagcc aggccgtcgc gcaggtgctc gatgcactgg tgccgtggct
     1381 cgagcaggaa gtcgagcgcg cgcgcagcgc cgggagctga cccgatgcgc gatcccgatc
     1441 ggccttcgtc ggcgcgcaat gtgctcggcg gaccgccggc gagttgctgc atgcggccgc
     1501 tgaccggctt ctttcgcgac ggttactgcc acaccggccc gcaggacgtg ggctcgcaca
     1561 cggtgtgcgt gcagatgacg gcggagttcc tggacttttc cgtcgcgcgc ggcaacgacc
     1621 tcgtcacgcc gcatccggag tccggctttc cggggctcgc gcccggcgac cgctggtgtg
     1681 tgtgcgcggc acgttggctc gaggcctacg aggccggcgc ggcggcgccg gtcgtgctcg
     1741 ccgccacgca agagaaggcg ctcgacatcg tgccgctcgc cgcgctgcgg gcgcacgcgc
     1801 tggatctgca ctgagccggc gatgcgcgcg cgcgccatgc tgctgcacgc cgccggccgc
     1861 ccgctcgcgg aaggcacgat cgacgtcggc gatcccggcc cgggcgaggt gcggctgcgg
     1921 gtttccacct gcggcgtgtg ccgaactgat ctgcacatcg tcgacggcga actcacgcag
     1981 ccgaagctgc cgctggtgcc ggggcacgag atcgtcggcc gcatcgaggc gatcggcgcc
     2041 ggcgtgacgc gcttgggcgt cggcgatcgc gtcggcgtgc cgtggctcgg cggcacctgc
     2101 ggccattgcc gctactgccg cgagcgccgg gagaatctgt gcgacacgcc gcggttcacc
     2161 ggctacacgc gcgacggcgg ctatgcggaa cgctcgctcg tcgacgccgg ctactgcttc
     2221 gcactgcccg acacgctcga cgacgcgcac gccgcgccgc tgctgtgcgc cgggctgatc
     2281 ggctatcgcg cgctgcgctt cgccggcgat gcacgcgtcc tcggcatcta cggcttaggt
     2341 gccgccgccc atctgctcgc gcaggtcgcc gtctggcagg ggcggcgcgt gttcgcgttc
     2401 acgcgtgccg gcgatgcgga cggccagcgc ttcgcgcgcg cgctcggctg cgcgtgggcc
     2461 ggcgcgtcgg acgagcttcc gcccgaaccg ctcgacgccg cgctgatctt cgcgccggtg
     2521 ggcgcgctgg tgccgaccgc gctgcgcgcc acgcgcaagg gcgccgtcgt cgtctgcgcc
     2581 ggcatccaca tgagcgcgat cccctcgttc gactacgagt tgctgtgggg cgagcggacg
     2641 atccgttcgg ttgccaacct cacgcgcgcc gacggtgacg aattcctcgc cctcgccgcg
     2701 caagtgccgg tgcgcacgca catcgagttg ttcgatctgg cgcgcgccaa cgaggcgatc
     2761 gacgccgtgc gcgccgggcg cgtgcacggc gccgcggtgc tcaggtgctc gtgaagccgg
     2821 acgcgccgcg cgtggtgctc gacaccaacg tgctgctcga cctgtgggtg ttcgacgatc
     2881 cgggcgtcgg ctcgctgcgc gcggccctcg gggcgggcgc gtacgtcgcg ctgcgctcgc
     2941 gggtgaccga caccgaagtc gccgatgtgc tggcacgccc gcagttcgcg ctgagcatcg
     3001 agcggcagcg ggcactgctg aaccggtggc gcgagtgcgc gcggccggtc gaggacatcc
     3061 gacgtgcgcc gtggcagtgc gacgacccgc acgaccagcc ttttctcgat ctcgccttca
     3121 ccgcgcgcgc acagggtctg gtcaccaagg acaaggcact gctgcggctg gcgcgcaagg
     3181 cgcgcagcac cggactgcgg atcgtcgcgc cgctgcgctt cgcggtagcc atgcccgcgt
     3241 gcacataggg gtcacgcgcg gtagcggggt tgatcgcggc cagcgaccgg cctatagatg
     3301 gcgccatcgc accgctaacg gaggtctcat gcgcagacgc ctgtacttcg tcctgccaga
     3361 tctgggcagc gcgatccaga ccgccaacga cctgctgctc tctcgggtgg aagaccggca
     3421 catgcacttc cttggccggc gcggcatgtc gctcggcgaa ttgcacgagg ccggcttcat
     3481 gcagaagtcg gacttccgcc acagcctgtt cctcggcacc gtgctcggcg cagtcggcgg
     3541 gctggtggtc ggcgtgatac tgaagctcac cgggatcggc gacatcggct ggctcatcat
     3601 cgccacgctg atcggcggcc tgttcggcgg ctggacatcg acgctgatcg gaatctcgac
     3661 gccgaaccac acactcaaac ccttcgcagc cgacatcgag gccggccgca tcctgctgat
     3721 ggtcgacgtg ccgccgagca gaatcgacga agtgaaggcg ctggttgcca agcgccaccc
     3781 cgaagtcgcc gatcgcggcg tcgagccgct gatgccggcg tttccgtgac ggtcaggccg
     3841 gcggcgcgag caacccgtgc cgcagtccgc gcgcgtcgtc gagcgcgtgg tggcggcgca
     3901 gccccagcgc tcgcacgacc tcgtcgaggt gttcgtacgc gaacggcacg tgcagccagc
     3961 cgacgttgtc gggcaggtcc tcggtgccga gcagccaggt cagcgactgg cggtcgtagc
     4021 cggagtcgca gacgatcgtc accggctggt cgcgtgatcg gtccgcgagc caggcgataa
     4081 acgcgtcgcg cgccatcatg agcggcaccg cgtcgccgtc gagcagcggc agcacgacgt
     4141 cgcgcacgaa ctcgctcgcc tgatcggccg gccagtcctg cagttcgcag taaaaggccg
     4201 tcgaatcggt cgccaccgcg ccgacgctga tcagggtcgg ctcgcgcagg ttggtgaatt
     4261 cgctgtcgaa gaacacgtac gtgcgcgcgc cttcgggcgt cagcgcggcc ggccgcggcg
     4321 gcagtgaccg cagcaacagc gtctcgtccg cgtattcgcc cgggccgagc cgcagttcct
     4381 tcaggatttc gcgcggatcg ccttcgccct ggcggaagcc cgcgcgccgc ccggcgcgca
     4441 gtgcgtgcac attgcggcgt gaggtgcgca gcacgaagcg gttgacgccg tactccgtct
     4501 gcagccactc gcacgcgagc gcgagcgcgt ccgacccgta gccgcggccc tggaagcgcc
     4561 gcgccgcgag ccaggcatcg atctcgacgc cgtcgcgcag caggttgatg tcgcggtagg
     4621 agacgaaacc gacgtcctcc gcgccgttcg aaatcagcaa agcgcgcccg gcaagcggcc
     4681 gcaggccgtt gaagaagtgc tcggcatagc gcgcacggaa ctcgtcgaat gtcggcaccc
     4741 gcggctcgaa cagcggggcg ttgccggtca tcgtctgtgc caggtcgttg cgcgcgatcc
     4801 agtgatagat gcgcatgcgg tcgtccggca gcgcgcgacg cagcgagatg cgggagttgc
     4861 tggcgacgat catcgacgct tcgatccgtg gtgcggccga ttgggttgcg cagagtatag
     4921 ttttcgcgct cccgccgcac ttcattccaa cgcatgccgc accatgccct gcccccgcgc
     4981 gcgctcgcgg acctgatcgg cccctcgcgc ctgccgaacg tcggcacgac gatcttcacc
     5041 gtgatgtcgg cgctggccgc caggcacggc gcggtcaacc tgggccaggg tttcccggat
     5101 ttcgactgcg atccgaagct gcagcagtac ttcaccgacg cgatggcggc cggccacaac
     5161 cagtacgcgc cgatggcggg cgtggcggcg ctgcggcagg cgctgagcgt caagcacgag
     5221 cagctgtacc ggcatcgcta cgatcccgac gccgagatca ccgtgaccgc cggcgccacg
     5281 caggcgatca tggccaccgt gctcgcgctg gtgcggcc
//
LOCUS       DBNHBY010000361         2961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_901192, whole genome shotgun sequence.
ACCESSION   DBNHBY010000361 DBNHBY010000000
VERSION     DBNHBY010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_901192"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..787
                     /locus_tag="AC16HB_08990"
     CDS             191..787
                     /locus_tag="AC16HB_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HIR5606964.1"
                     /translation="MPFLLPMLDQFASDTAVALLNRLLARESWARDRLAPFGGRGARI
                     ECGPFAVQLGVRDGGLLEATGNAPDVTISMDPAALPQALLEPRAIMRNVRLDGDAEFA
                     QALSYVLQNLRPEPEEELSRFVGDAAAQRIVGFLRAAFAQAQEAGTRLATTAADYFVA
                     ENPLLVARAEADAFARDVAALRDDVERLAKRIELLASR"
     gene            787..2355
                     /gene="ubiB"
                     /locus_tag="AC16HB_08995"
     CDS             787..2355
                     /gene="ubiB"
                     /locus_tag="AC16HB_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR5606965.1"
                     /translation="MRLFRLLRILFAVFRFGLDEIALSSLKSPFLQKLVRVVTLGRRL
                     REPRGVRLRRALEDLGPIFVKFGQVLSTRRDLLPVDIADELARLQDKVPPFDSDLAVR
                     EIERGLGRRLDELFSEFDRTPVASASIAQVHFATLRGGAHDGRPVAVKVLRPGMRAAI
                     DKDLALLEVGAALVERLWRDGKRLRPRAVVAEFSKYLHDELDLMREAANASQLRRNFA
                     ASNQLRVPEMIWDHCCENVLVMERMGGIPVSQVDRLRATGIDIKKLSADAVEVFFTQA
                     LRDGFFHADMHPGNIYVGDSGEDFGRWIALDFGIVGTLSDNDKNYLAQNFLAFFRRDY
                     KRVAELHVESGWVPPATRVDELESAIRAVCEPIFDRPLKDISFAQVLLRLFEASRRFN
                     VEVQPQLVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLERWMQTQVGFKGLEEKLRHE
                     AVQWSHLLPQLPRLMHQALTDTSHHDRMYAELRALRQETQRRNRLLLIGVTCLAAFAL
                     MLAWGLLGFQLPGR"
     gene            2352..>2961
                     /locus_tag="AC16HB_09000"
     CDS             2352..>2961
                     /locus_tag="AC16HB_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606966.1"
                     /translation="MSAIRRSVLAGVVIALAGCGGGGGSAPAQPLVCADSAAAFSVYA
                     DASVVVGKAAGAVIAGCSGPLANVRWTQTAGPAVTLISDRTQAISFEPPSAGRYSFRV
                     DFLDPSGTAQSTSVSVDAMPPSVEVRVLARADQAVRKGGTVSLRAWPPAGADEQTTWQ
                     QLDGPATTLDTSDPNRIILTAPDVGADTALRFRVTRRTASGGT"
BASE COUNT          437 a         1005 c         1045 g          474 t
ORIGIN      
        1 agcgatgcga acgagcatct ggcgagcgtg cgcttcaccg gtacgctgcg cgtcgatggt
       61 gaactcgagc agttcgacga ggtctggaac ctcaccaagt ccaccgacgg caagaccggc
      121 tggctgctgg cgggcatcca gcaactcgct taggcgcgca tcgcatctgc gacaatcagg
      181 gcaccttcgg gtgccctttt tgctgccgat gctcgatcaa ttcgcttccg acaccgcggt
      241 cgcgctgctc aatcgcctgc tcgcgcgcga aagctgggcg cgcgaccggc tggcgccatt
      301 cggcggacgc ggcgcacgga tcgaatgcgg gccctttgcc gttcaactcg gcgtgcgcga
      361 cggcgggctg ctcgaggcga cgggcaatgc gcccgacgtg acgatctcga tggacccggc
      421 ggcgctaccg caagcattgc tcgagccgcg cgcgatcatg cgcaatgtgc ggctcgacgg
      481 cgacgccgag ttcgcgcaag cgctttcgta tgtgctgcag aacctgcggc ccgagcccga
      541 ggaagagctg tcgcgcttcg tcggcgatgc ggccgcgcag cgcatcgtcg gcttcctgcg
      601 cgccgcgttc gcgcaggcgc aggaggccgg cacgcggctg gcgaccactg cggctgacta
      661 cttcgtcgcc gaaaacccgc tgctggtcgc gcgcgccgag gccgatgcct tcgcgcgcga
      721 cgtggccgcg ctgcgcgacg acgtcgagcg gctggccaag cgcatcgagc tgctcgcgtc
      781 gcgctgatgc gcctgttccg gctgctgcgg atcctgttcg ccgtgttccg gttcgggctg
      841 gacgagatcg ccctgtcgag cctgaagagc ccgttcctgc agaagctggt gcgcgtcgtc
      901 acgctgggcc ggcgcctgcg cgagccgcgc ggcgtgcgcc tgaggcgcgc gctcgaagac
      961 ctcggcccga tcttcgtcaa gttcggccag gtgctgtcga cgcggcgcga cctgctgccg
     1021 gtcgacatcg ccgacgagct cgcccggctg caggacaagg tgccgccgtt cgattcggac
     1081 ctcgcggtgc gcgagatcga gcggggcctc ggccgcaggc tcgacgagct gttctccgag
     1141 ttcgatcgca cgccggtggc gagcgcgtcg atcgcgcagg tgcatttcgc aacactcagg
     1201 ggcggtgcgc acgacggccg cccggtcgcg gtcaaggtgc tgcggccggg catgcgcgcg
     1261 gcgatcgaca aggacctggc gttgctcgag gtcggcgccg cgctggtgga gaggctgtgg
     1321 cgcgacggca agcgattgcg cccgcgcgcg gtggtggccg aattcagcaa gtacctgcac
     1381 gacgaactcg acctgatgcg cgaggcggcc aacgcctcgc agctgcggcg caacttcgcc
     1441 gcctcgaacc agctgcgcgt gccggagatg atctgggacc actgctgcga gaacgtgctg
     1501 gtgatggagc gcatgggcgg cattcccgtg agccaggtcg accggctgcg cgcgaccggc
     1561 atcgacatca agaagctgtc ggccgacgcg gtcgaggtgt tcttcacgca ggcgttgcgc
     1621 gacggcttct ttcatgccga catgcatccg ggcaacatct atgtcggcga ttcgggcgag
     1681 gacttcggac gctggatcgc gctggacttc ggcatcgtcg gcacgctgtc cgacaacgac
     1741 aagaactacc tcgcgcagaa cttcctcgcg ttcttccggc gcgactacaa gcgcgtggca
     1801 gaactgcacg tcgagagcgg ctgggtgccg ccggccacgc gcgtcgacga actcgaaagc
     1861 gcgatccgcg ccgtctgcga gccgatcttc gaccggccgc tgaaggacat ttcgttcgcg
     1921 caggtgctgc tgcgcctgtt cgaagccagt cgccgcttca acgtcgaggt gcagccgcag
     1981 ctcgtgctgc tgcagaagac gctgctgaac atcgaagggc tcgggcgcca gctcgacccg
     2041 gacctcgacc tgtggaagac cgccaagcct ttcctcgagc gttggatgca gacgcaggtc
     2101 ggtttcaagg gcctcgagga gaagctgcgc cacgaggcgg tgcagtggag tcatctgctg
     2161 ccgcagctgc cgcggttgat gcaccaggcg ctgaccgaca cctcgcatca cgaccgcatg
     2221 tatgccgagt tgcgcgcact gcggcaggag acgcagcggc gcaaccgact gctgctgatc
     2281 ggcgtcacgt gcctggcggc gttcgcgctg atgctggcgt ggggtctcct cggctttcag
     2341 cttccggggc gatgagcgcg atccggcgca gcgtactggc gggcgttgtc atcgccctcg
     2401 ccggctgcgg cggaggcgga ggcagtgcgc cggcgcagcc gctggtctgc gccgacagcg
     2461 cggcggcctt ctcggtctat gccgacgcct ctgtcgtcgt cggcaaggcc gctggcgcgg
     2521 tgatcgcagg gtgcagcggc ccgctcgcga acgtgcgctg gacgcagacc gcaggtccgg
     2581 ccgtgacgtt gatctcggat cgaacccagg cgatcagctt cgagccgccg tcggccggta
     2641 ggtactcgtt ccgggtcgac ttcctcgatc catccggcac ggcgcaatcg accagcgtca
     2701 gcgtggatgc aatgccgccg tccgtggaag tacgggtcct tgcgcgcgcc gatcaggcgg
     2761 tgcgcaaggg cggcaccgtg tcgctgcgcg cctggccgcc cgccggcgcg gacgagcaga
     2821 cgacctggca gcagctcgac ggccccgcga cgacactcga cacgtcggac ccgaatcgaa
     2881 tcatcctcac cgcgcccgac gtcggcgccg acacggcgct gcgctttcgc gtcacccgcc
     2941 gcacggcgag cggcggcacc g
//
LOCUS       DBNHBY010000362         2035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1780379, whole genome shotgun sequence.
ACCESSION   DBNHBY010000362 DBNHBY010000000
VERSION     DBNHBY010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1780379"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="AC16HB_09005"
     CDS             <1..1796
                     /locus_tag="AC16HB_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR5606967.1"
                     /translation="GADEDATLNWPVTSGIRLATGRLGRAEVRIGSLAVWLDDDSDVE
                     FTRVDDEAIQITALHGSLALRVRNREMLRELDLLTPRERIVIEDIGRYRIDVDRAGAT
                     TNVTAFPGQARILNGRMIFTVPSGQRGDVEAAPVTSFRLSPALVDPFDDWVAARERRD
                     ETPRSAQYVSPEMTGVEALDDQGTWRPVESYGPVWFPSYVPTGWVPYRYGRWAYIAPW
                     GWTWIDDAPWGFAPFHYGRWVVIGGVWGWVPGVIVPRPVYAPALVAWFGSPGVSVSIG
                     IGAPIGWFPLGPREVFIPPYHCSRRYVNFVNVPHVTNITNITVVNPPAHYVNRDRDRT
                     TWVPGNALVNRGPIQRVVAPPPADWANRAPSVRPPIEQPHDIKKRPTPVPLPGRERPG
                     ERTPPSARQVMPPAADGPAPSAPAVPRPGIDESRRRAPRTERPEVPAARAPAPGANAP
                     GPTTPPQGPRVAPHDERRQATPPSEDATRMRQPAPTPQIRQQPTEPAPRAQERNHGSP
                     RAIPQPAPQAAPAAPQTRQQPVEPAPRAQERSHGSPRAIPQPAPPATPAAPQMAPQPQ
                     RSGNQVRQHEARAAREERPPAQPEHGSRGRD"
BASE COUNT          345 a          736 c          675 g          279 t
ORIGIN      
        1 ccggagccga cgaagacgcg acattgaact ggccggtcac ttccggcatc cggttggcga
       61 cgggccggct cggccgcgcc gaagtgcgca tcggctcact cgcggtctgg ctcgacgacg
      121 actccgacgt cgaattcact cgcgtcgacg acgaggcgat ccagatcacc gcgctgcacg
      181 gcagcctcgc gttgcgcgtg cgcaatcgcg agatgctgcg cgaactcgac cttttgacgc
      241 cgcgtgaacg catcgtgatc gaggacatcg gccgctatcg catcgatgtc gatcgtgcgg
      301 gcgccacgac aaacgtgaca gcctttcccg gccaggcccg catcctgaac gggcgcatga
      361 tcttcaccgt tcccagcgga cagcgcggcg acgtcgaggc cgctccggtc acgtcgtttc
      421 gtctgagccc ggcgctggtc gatccgttcg acgactgggt cgcggcgcgc gaacgacgcg
      481 acgaaacacc acgcagcgcc cagtacgtct cgcccgagat gaccggcgtc gaggcactcg
      541 acgaccaagg cacgtggcgc ccggtcgaat cgtatggacc ggtatggttc ccatcgtatg
      601 tgccgaccgg ctgggttcct tatcgctatg gacggtgggc gtacatcgcc ccgtggggct
      661 ggacatggat cgacgatgcg ccgtggggat tcgcgccctt tcactatgga cgctgggttg
      721 tcatcggcgg cgtgtggggt tgggtgccag gcgtgatcgt gccgcgtccg gtctatgcgc
      781 cggcgctggt ggcgtggttc ggcagtccgg gcgtcagcgt gtcgatcggc atcggcgcgc
      841 cgatcggctg gttcccgctc ggcccacgcg aagtcttcat tccgccgtac cactgctcgc
      901 gacgctacgt gaacttcgtc aacgttccgc acgtgaccaa catcacgaac atcacggtgg
      961 tcaatccgcc agcgcattac gtgaaccgcg accgagaccg cacgacatgg gtgccaggta
     1021 acgccctggt caaccgcgga ccgatccagc gcgtcgtcgc gccgccgccg gcggattggg
     1081 caaatcgcgc gccgagcgtt cgcccgccca tcgagcagcc gcatgacatc aagaagcggc
     1141 cgacaccggt gccgctccct ggacgcgagc gtccgggcga gcggacgcca ccgtcagcgc
     1201 ggcaagtcat gccgcccgcg gccgacggcc cggcaccgag cgcacctgca gtcccccgtc
     1261 cgggcatcga cgagtcgcga cgccgtgcgc cccgcaccga acggcccgag gtgcctgccg
     1321 cgcgtgcgcc cgcccccggt gctaacgcgc ctgggccgac aaccccaccg cagggaccgc
     1381 gcgtcgcacc gcacgacgaa cgacgccagg cgacgccgcc gagcgaagac gccacacgga
     1441 tgcgccagcc ggcccccacg ccgcagattc gacagcagcc gacggaaccc gcgccgcgcg
     1501 cccaggaacg caaccatggc agtccacgcg cgataccgca gcccgcgcct caggccgcac
     1561 cggctgcgcc gcagactcgc cagcaaccag tggaaccggc gccccgcgcc caggaacgca
     1621 gtcacggcag tccacgcgcg atcccgcagc ccgcgccgcc ggcgacaccg gcggcgccgc
     1681 aaatggcacc gcagccgcag cgcagcggaa atcaggtcag acaacatgaa gcgcgcgctg
     1741 cgagagagga acgcccgccc gcgcaaccgg aacacggcag tcgcgggcgc gattgagttc
     1801 agcgacgcgc gcgaccggcg atacgatcaa tatgggtatg ggcgcgcatc gtcctgaacg
     1861 gcgatgcaca gtccctgcct ttgtgctaag cagggtgcgc aagcaacgtc gcgcacgcag
     1921 ttagggaggt cggcgctatg aagctcgaac ggttcggccc tgaactcctt ctgtccatcg
     1981 ccgcatcggt tttcgccgtc ggcgcgccga ttccggcggc ggcgcaggag ggcgg
//
LOCUS       DBNHBY010000363        10303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1577878, whole genome shotgun sequence.
ACCESSION   DBNHBY010000363 DBNHBY010000000
VERSION     DBNHBY010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1577878"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16HB_09010"
     CDS             <1..352
                     /locus_tag="AC16HB_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR5606968.1"
                     /translation="PGGAGFAWKDITVVKLGADWAMRPDLTLRAGYTRGDQPIPNSDT
                     FFNILAHGVREDHYTLGATWTLAGKSELTFAFMYAPTVTVNGSNSIPAPFGGGNVNLR
                     MRQMQFGVAYGWKF"
     gene            complement(414..587)
                     /locus_tag="AC16HB_09015"
     CDS             complement(414..587)
                     /locus_tag="AC16HB_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606969.1"
                     /translation="MNKLLAALMTAAFVAAPAFAQDKKADAKPTMEQCKKDPKMKGCD
                     EVLKKEKKAKCGC"
     gene            complement(694..1290)
                     /locus_tag="AC16HB_09020"
     CDS             complement(694..1290)
                     /locus_tag="AC16HB_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR5606970.1"
                     /translation="MTLPLYGFDLITESQGLITALLLGFGFGWFLERAGFGSARKLAS
                     QFYLHDMTVLKVMFTAIVTTAVGLWFFAAIGWLDLSYVYLTPTSFGAQLFGGLLLGAG
                     FILGGFCPGTSVTATATGSGDGLLYMSGFVAGLAIYAVALPSWELWANANSVGELTLP
                     QWFGIPYGAVVLGVVVLALAAFAGAEWTERRFRHLRPQ"
     gene            complement(1287..1850)
                     /locus_tag="AC16HB_09025"
     CDS             complement(1287..1850)
                     /locus_tag="AC16HB_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR5606971.1"
                     /translation="MSAAAIASAEGRGTEKVSVAEGRRLMNPYLAGFGLGLVLLLTFL
                     IMGRGLGATAGYGSLLAAAFGAFAPDWTAANPALKNYFADPPATNWTLYLLIGAFAGA
                     LVSGLLARRVHWRIERGPNLSIRMRLLFAFSGGLLAAIGAKLAKGCTSGQALTGGSQL
                     NVGSWVFMLSVFAGGYLLAYVVRRLWR"
     gene            complement(1992..3371)
                     /locus_tag="AC16HB_09030"
     CDS             complement(1992..3371)
                     /locus_tag="AC16HB_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5606972.1"
                     /translation="MRTAPLTVAIALACASAPAFAQAPAPAPKATQPAVCNNCHKPEA
                     GFVAGYFENVAFKSQSIQLGIDAAKEIVRFDEKALKVLDAGEAKKPEFLRDARKGHEV
                     RIAYLEKDGQKWASEIRFKGPVKVAKEDLVDYAFVRKLADQGSGNFALIDSRPLPRFQ
                     QGTIPGAINVPYPAWDKFVNRLPADKNALLVFFCQGVTCQMSPLSQRKAIALGYKTTK
                     VYHEGVPEWQTKDYLATRPEFVKEAYIDKDIPAVLLDVRAREESASGHIKGAVGMPPA
                     VVKKSLKAFPDPKLNAPIIVYDGRGGADAVAVARQLIKAGQKNVQVMEKGLLGWQSAG
                     YAIESGVPVLTKVAYVPKPRPGSIAADEFNKLARATPADVLILDVRNPDEANAGMIKG
                     ALLIPDEELAARIKELPKDKRIIAHCSTGIRAEMAYHKLKDAGYRAGFLNAEIAIAKD
                     GSFKVTPKL"
     gene            complement(3407..4456)
                     /gene="nrfD"
                     /locus_tag="AC16HB_09035"
     CDS             complement(3407..4456)
                     /gene="nrfD"
                     /locus_tag="AC16HB_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5606973.1"
                     /translation="MLEITTTRHNPLVDPHLAVWSWEIPLYLFVGGVVAGLMILGGTA
                     MLRLARGEDPKRYFSLHAPWLAFMLLNLGMAALFLDLAHKLYVWAVYLTLQPAAPMSW
                     GSWVLIIVYPLLLASALIRLPEAWPWLAQRAPVLKRWSDALLARPKTIGFLGWINIAL
                     GIALGIYTGILLSTMVARPLWNLSILGPLFLVSGLSAGAALLHLMTAVLPPTRPAPKG
                     LIGGAIGALVQPLGATAPEKGTVNALIRADIAFLAAELLLLGLLLVGLLTATASHAAA
                     FDLLMSGKYAFAFWGVLVVLGLLVPLLLQGLELGHRMPHTVVPAVLVLVGGYTLRWLM
                     VNAGQMSEIVSTAAR"
     gene            complement(4467..5006)
                     /locus_tag="AC16HB_09040"
     CDS             complement(4467..5006)
                     /locus_tag="AC16HB_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5606974.1"
                     /translation="MARFGMVIDTRKCVGCMDCVIACQTENDVPVGYARDWITTEVRG
                     EFPHLSMEIRSERCNHCDNPPCVSCCPTGASHVSDLGRIVKVKASRCIGCKACVSACP
                     YGARFVHPQGYADKCTFCDHRVKEGKDPACVSVCPTHCMAFGDLDDPKSRVSELLRTR
                     KWHTLLPEAGTKPRVFYLT"
     gene            complement(5008..7209)
                     /locus_tag="AC16HB_09045"
     CDS             complement(5008..7209)
                     /locus_tag="AC16HB_09045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5606975.1"
                     /translation="MSNSLSRRSFLKISAATFGAAATTTQIPPLARAIAADLPAGAGV
                     ETVPTFCEMCFWRCGGIAYVRGGKLWKFEGNPIDPQSRGRLCPRGTGAVGSYYDPDRL
                     QKPLIRAGERGKEAWKTASWDEAFTYIAERMNRIKAQHGPESVAMFNHGIGVRFIQHV
                     LKSWGVTSFAGPSFAQCRGPRDVGFTLTFGSGLGSPESTDIANTDCLVLIGSHLGENM
                     HNSQVQEFARAVERRIPIIVVDPRFSVAASKAKYWLPIRPGTDMALLLAWMNVLVNEG
                     RYDKAFVDQHGKGFDRFVQEIKPYTPQWAESETDIKAETIRATALEMANARPATLVHP
                     GRRVNWYGDDAQRSRAVALLNALLGSWGRKGGLYVQAGMKVASYPLPAYPKSDQPLVD
                     NPNRERYPFADEAITTGIRDATLTGKPYPVKGWFVYSSNIFQALPNQAETLRAIQSLD
                     LLVVIDTAPSEIAGYADVVLPEVTFLERYDELLAGWGRRGWLALRQPVMPAPHEQKPG
                     WWIAKQLANKLGIPECMPFNDIEEYLRMRVEKSGHDWGRLKKEGVILAEPQPITVEEG
                     LALSFDTPSGKVEFYSDQLKDKGFDPVPKYTKHPTAPDGYFRLISGRAPVHTFSRTQT
                     NRLLGDAMRENEVWINAATARKAGLASGQYVKLKNQDGVVSNRVRVKATQRIRPDTVY
                     MVYGFGHTNRALKGAYLKGASAAQLNTHYATDPLMGGTSIHGNFVTFVKEA"
     gene            complement(7515..7955)
                     /locus_tag="AC16HB_09050"
     CDS             complement(7515..7955)
                     /locus_tag="AC16HB_09050"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5606976.1"
                     /translation="MKRFMLALSLAAALVVGLPAAAPPAAATGGEIRTVYHINEIEKA
                     RGLLANVRNHLRDDPTAKIVVIANGAGIEFLLKDAIDKNGMPFAPALEELARQGVSFK
                     VCRNTLTARKLTDAAVTEEAGVVQAAVAEIARLQAREGYAYIKP"
     gene            8340..8828
                     /locus_tag="AC16HB_09055"
     CDS             8340..8828
                     /locus_tag="AC16HB_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5606977.1"
                     /translation="MDATRLLFAVLLAVSAAAPAQERRNWFNDPFERATAGLPGCPVP
                     QGPLLTEAEQKREAHYRIERGTTCWLAKKCDEPNAYKRDPEINAAAAAAIRADPTLRD
                     TSVWVITQRKFVFVQGCVRARAQIKRIAAAVRADPRVEYVGDEVLIGTRGKPPYPTTG
                     SK"
     gene            8825..9502
                     /locus_tag="AC16HB_09060"
     CDS             8825..9502
                     /locus_tag="AC16HB_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606978.1"
                     /translation="MSGRHGARQTLRASSTGLTARELAVLRRLGTPAKIQTFLNRIPI
                     NHEHDGETVLSVREVLRQRRAHCIEGAFVAATALWLHGEPPLVMHMDCDLSDYPHVIA
                     VYRRDGCWGAISKTNGSRLRFRDPVYRTLRELALSYFHEYTNKRGEKTLRSYTAAFDL
                     RRIDPALWVTNPASCWEAHDRLEALRHYPLLAPRQIQALARSDPFERRVGKVEQYPAP
                     TSLARRG"
     gene            complement(9506..10240)
                     /locus_tag="AC16HB_09065"
     CDS             complement(9506..10240)
                     /locus_tag="AC16HB_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5606979.1"
                     /translation="MQKMHAAAAAGAFLCLVSVARADPADDVLSPQVDQGEREFELKV
                     GTQTKKDEPRFSAATLGIGYGALSWWAVELELKYEREGAATHFDAVELENRFQLTETG
                     KFPFDLGLLFEIEWPRDRAEGWEFAWGPLLQAEAGRMQYNANLLWKRRIRGEEPAATE
                     LGYRLQAKYRWREALEFGAQAFGDFGPWRHWSPASQQSHRIGPALFGRTRLAPGHALK
                     WNAAYLFGTTRSSPDRALRAQFEYEF"
BASE COUNT         1728 a         3474 c         3347 g         1754 t
ORIGIN      
        1 tcccggcggt gcggggttcg cgtggaagga catcacggtg gtgaagctcg gcgccgactg
       61 ggcgatgcgc ccggacctga cgctgcgcgc tggctacacc cgcggcgacc agcccattcc
      121 gaacagcgac acgtttttca acattctcgc gcatggcgtg agggaggatc actacacgct
      181 cggcgcgacc tggacgctcg cgggcaagtc cgaactgact tttgcgttca tgtatgcgcc
      241 gaccgtgacg gtcaacggat cgaattcgat cccggcgcct tttggcggcg gcaatgtcaa
      301 cctgagaatg cggcagatgc agttcggcgt tgcttacggc tggaaattct gagccatcgg
      361 aggaatgaaa acgggccgca catgcggccc gctttctttg ctcgtggtgc tgctcagcag
      421 ccgcacttgg ctttcttctc ttttttgagc acttcgtcgc agcccttcat cttcgggtct
      481 ttcttgcact gttccatcgt cggcttcgca tcggccttct tgtcctgcgc gaaggcggga
      541 gctgcgacga atgcggcggt catcagggcg gccagaagct tgttcattgg agcgactcct
      601 tcggatcgag ttgggcggga cactttgcgt tagcgcatgg ggccggtcaa gggttgaccg
      661 ggcccgtttc gatctggatc aaccgcgatc gggctattgc gggcgtaggt ggcggaacct
      721 gcgctcggtc cactcggcgc cggcgaaggc cgcgagcgcg agcacgacga cgccgagcac
      781 gaccgcgccg tacgggatcc cgaaccattg cggcagcgtc agctcaccga cgctgttcgc
      841 gttggcccat agttcccacg acggcaacgc gacggcgtat atcgccagtc cagcgacaaa
      901 gccgctcatg tacaacaagc cgtcgccgct gccggtcgcg gtcgccgtga ccgacgtgcc
      961 ggggcagaag ccgccgagga tgaagcctgc gcccagcagc agtccgccga ataactgcgc
     1021 cccgaaactc gtcggcgtca ggtacacgta cgagaggtcg agccaaccga tcgcggcgaa
     1081 gaaccacagc ccgaccgccg tcgtgacgat cgcggtgaac atcaccttca gcaccgtcat
     1141 gtcgtgcaga tagaactgcg acgccagctt gcgggcgctg ccgaagccgg cacgctcgag
     1201 aaaccagccg aagccaaagc cgagcagcag cgccgtgatc aatccctgcg attccgtgat
     1261 caggtcgaag ccatacagtg gcagcgtcat cgccagagcc tccgcacgac gtacgccagc
     1321 aggtagccgc cggcaaagac cgacagcatg aacacccagc tgccgacatt gagctgcgat
     1381 ccgcccgtca gcgcctggcc gctggtgcag cccttggcaa gcttcgcacc gatggcagcg
     1441 agcagcccgc cgctgaacgc gaacagcagg cgcatgcgaa tcgacaggtt cggtccgcgc
     1501 tcgatgcgcc agtgcacgcg cctcgcgagc aggccggaca cgagcgcgcc ggcaaaggcg
     1561 ccgatcagca gatacagcgt ccaattggtc gccggcggat cggcgaagta attcttcagc
     1621 gccggattgg ccgcggtcca gtcgggcgca aacgcgccga acgcggccgc cagcagcgag
     1681 ccgtagcccg ccgtggcgcc cagcccgcgg cccatgatca gaaacgtcag cagtaacacc
     1741 agtccgagtc cgaatcccgc aaggtaggga ttcatcagcc gacgtccctc ggcgacggac
     1801 accttctcgg tgccgcgtcc ttccgccgat gcgatggcag cggcgctcat agtcgactcc
     1861 tcaagcgatg aagtttcaat gcactcatga tgcgccgcac aaccaggaaa cggtgtgatg
     1921 ggcatcacgc aggaaactca gtggaggaga ctgcgtgatg cgccaccaaa ccgcagggcc
     1981 gaagcgaacg gtcacagttt cggcgtcacc ttgaacgaac cgtccttcgc gatcgcgatc
     2041 tcggcgttca ggaagcccgc cctgtagccg gcgtccttca gcttgtgata cgccatctcg
     2101 gcgcggatgc cggtcgagca gtgcgcgatg atgcgtttgt ccttcggcag ttccttgatg
     2161 cgcgcggcga gttcctcgtc cggaatcagc agcgcgccct tgatcatgcc ggcgttggct
     2221 tcgtccgggt tgcgcacgtc gagaatcagc acgtcggccg gcgtcgcgcg tgcgagcttg
     2281 ttgaactcgt cggccgcgat cgatccgggc cgtggtttcg gcacataggc gaccttggtc
     2341 agcacaggca cgccgctttc gatcgcatag cccgccgact gccagccgag caggcccttt
     2401 tccatcacct gcacgttctt ctggccggcc ttgatcagtt gccgcgccac cgcgactgca
     2461 tcggcgccgc cgcgaccgtc atagacgatg atcggcgcat tgagcttcgg atcggggaaa
     2521 gccttcaggc tcttcttcac gactgccggc ggcatgccca ccgcaccctt gatgtgcccg
     2581 ctcgcgctct cttcccgcgc gcgcacgtcg agcagcactg cggggatgtc cttgtcgata
     2641 tacgcttcct tcacgaactc ggggcgcgtg gcgagatagt ccttggtctg ccactcgggc
     2701 acgccctcgt gatagacctt ggtggtcttg tagccgagtg cgatcgcctt gcgctgcgac
     2761 agcgggctca tctggcaggt cacgccctgg cagaagaaca ccagcagcgc attcttgtcg
     2821 gcgggcaggc gattgacgaa cttgtcccat gccggatacg gcacgttgat cgcgccggga
     2881 atcgtgccct gctggaaacg cggcaacggc cgcgagtcga tcagcgcgaa attgccgctg
     2941 ccctgatccg cgagcttgcg cacgaacgca tagtcgacca gatcctcctt cgcaaccttg
     3001 accgggccct tgaagcggat ctcgctggcc cacttctggc cgtccttttc caagtacgcg
     3061 atgcgcacct cgtgtccctt gcgcgcgtcg cgcaggaact cgggcttctt cgcctcgccg
     3121 gcatcgagca ccttgagcgc cttctcgtcg aagcgcacga tctccttcgc cgcgtcgatg
     3181 ccgagctgga tcgactgcga cttgaaggcg acgttctcga agtagccggc aacgaagccc
     3241 gcctcgggct tgtggcagtt gttgcagacc gcaggttgcg tggctttcgg cgccggcgcg
     3301 ggtgcctgtg cgaacgcggg cgccgacgca cacgccagcg cgatcgccac agtcaagggt
     3361 gcggttctca tcgttttcct ccagtcatga cctcgaaaga atttcttcag cgtgccgccg
     3421 tactgacgat ctcgctcatc tgcccggcgt tgaccatcag ccagcgcagc gtgtagccgc
     3481 cgacgagcac gagcacggcc ggcacgaccg tgtgcggcat gcgatggccg agttcgagcc
     3541 cctgcagcag cagcggcacg agcaggccca gcacgaccag cacgccccag aacgcaaatg
     3601 catactttcc gctcatcaac aggtcgaacg ctgctgcatg tgacgcggtc gcagtgagca
     3661 gtccgacgag caacaggccc agcagcagca gctcggccgc gaggaaggcg atgtcggcgc
     3721 ggatgagcgc attgaccgtg cctttctcgg gcgcggtcgc cccaagcggc tgcaccagcg
     3781 cgccgatcgc gccgccgatg aggcccttcg gtgccggtcg cgtcggcggc agcaccgcgg
     3841 tcatcaggtg cagcagcgcc gcgccggccg acagtccgga cacgaggaac agcggcccga
     3901 ggatcgacag gttccacagc ggccgcgcca ccatcgtcga cagcaggatg ccggtgtaga
     3961 tgccgagtgc gatgccgagc gcaatgttga tccagccgag aaatccgatc gttttcggcc
     4021 gcgcgagcag agcatcggac cagcgcttga gcaccggggc gcgctgcgcg agccacggcc
     4081 acgcctccgg caggcggatc agcgcggagg cgagcagcag cgggtagacg atgatcagca
     4141 cccacgagcc ccacgacatc ggcgcggccg gctgcagcgt caggtacacc gcccacacgt
     4201 acaacttgtg cgcgagatcg aggaacagcg ctgccatgcc gaggttcagc agcatgaacg
     4261 cgagccacgg cgcatgcagc gagaagtagc gcttcgggtc ctcgccccgg gccaggcgca
     4321 gcatcgcggt accgcccagg atcatcagcc cggcgacgac gccaccgacg aacaggtaca
     4381 gcgggatctc ccagctccac acggccagat gcggatcgac caaggggttg tgccgggttg
     4441 tcgtgatctc gagcatggcc tgtcccttac gtcaggtaga acacgcgcgg cttggtgccc
     4501 gcttcgggca gcagcgtgtg ccatttgcgc gtgcgcagca gttcgctgac ccggctcttc
     4561 ggatcgtcga ggtcgccgaa ggccatgcag tgcgtcgggc acaccgacac gcacgccggg
     4621 tccttgcctt ccttgacgcg gtggtcgcag aacgtgcact tgtcggcgta gccctgcggg
     4681 tgcacgaagc gcgcgccgta cgggcacgcc gacacgcacg ccttgcagcc gatgcagcgg
     4741 ctcgccttga ccttgacgat gcggccgagg tcggagacgt ggcttgcccc ggtcgggcag
     4801 caggaaacgc acggcgggtt gtcgcagtgg ttgcagcgct ccgagcggat ctccatcgac
     4861 aggtgcggga actcgccgcg cacctcggtc gtgatccagt cgcgcgcata gccgaccggc
     4921 acgtcgtttt ccgtctggca tgcgatcacg cagtccatgc agccgacgca cttgcgcgtg
     4981 tcgatcacca ttccgaaccg tgccatctca cgcctccttc acgaacgtca cgaagttgcc
     5041 gtggatgctg gtgccgccca tgagcggatc ggtcgcgtag tgcgtgttca gttgcgccgc
     5101 gctggcgccc ttcagataag cgcccttcag cgcgcggttc gtgtgaccga agccgtagac
     5161 catgtacacg gtgtcggggc ggatgcgctg tgtcgccttc acgcgcacgc ggttgctgac
     5221 cacgccgtcc tggtttttca gcttcacgta ctggcccgac gccaggcccg ccttccttgc
     5281 ggtggcggcg ttgatccaga cctcgttctc ccgcatcgcg tcgccgagca gccgattggt
     5341 ctgcgtgcgg ctgaacgtat gcaccggcgc gcgcccggaa atcagccgga agtagccgtc
     5401 cggcgcggtc gggtgcttcg tgtacttggg caccggatcg aagcccttgt ccttcagctg
     5461 atccgaatag aactcgacct tgcccgacgg cgtgtcgaac gaaagcgcaa ggccttcttc
     5521 gacggtgatc ggctggggct cggcgaggat cacgccctcc ttcttcagcc tgccccagtc
     5581 gtggccggac ttctccacgc gcattcgcag gtattcctcg atgtcgttga acggcatgca
     5641 ctcgggaatg ccgagcttgt tggccagttg cttggcgatc caccagcccg gcttctgttc
     5701 atgcggcgcc ggcatcaccg gctgccgcag cgcgagccag ccgcgtcgac cccagccggc
     5761 gagcaactcg tcgtagcgct cgaggaacgt cacctcgggc agcaccacgt cggcgtagcc
     5821 ggctatctcg ctcggagccg tgtcgatcac gaccagcagg tcgagcgact ggatcgcgcg
     5881 caaagtctcg gcctggttcg gcagcgcctg gaagatgttc gacgagtaga cgaaccagcc
     5941 cttgaccgga tacggcttgc cggtgagcgt cgcgtcgcgg atgccggtgg tgatcgcctc
     6001 gtcggcgaac ggatagcgct cgcggttcgg gttgtccacg agcggctggt ccgacttcgg
     6061 atacgcgggc agcgggtagc tcgcgacctt catgccggcc tgcacgtaga ggccgccctt
     6121 gcggccccag ctgccgagca gtgcgttcaa cagcgccacc gcacggctgc gctgcgcgtc
     6181 gtcgccgtac cagttcaccc gccggcctgg atgcacgagc gtggcgggcc gcgcgttggc
     6241 catctcgagc gcggtggcgc ggatcgtctc ggccttgatg tcggtctcgg actcggccca
     6301 ttgcggcgtg tacggcttga tttcctgcac gaacctgtcg aagcccttgc catgctggtc
     6361 gacgaacgcc ttgtcgtacc gcccctcgtt gaccagcacg ttcatccatg ccagcagcag
     6421 cgccatgtcg gtgccgggcc ggatcggcag ccagtacttg gccttgctcg ccgcgaccga
     6481 gaagcgcgga tcgacgacga tgatcggaat ccgccgctcg accgcgcgcg cgaactcctg
     6541 cacctgcgag ttgtgcatgt tctcgcccag gtgcgagccg atcagcacca ggcagtcggt
     6601 gttggcgatg tcggtcgact cgggcgagcc gagcccgctg ccgaaggtca gcgtgaagcc
     6661 cacgtcgcgc ggcccgcggc actgcgcgaa cgatgggccg gcaaagctgg tcacgcccca
     6721 gctcttcagc acgtgctgga tgaagcgcac gccaatgccg tggttgaaca tcgccaccga
     6781 ctccggcccg tgctgcgcct tgatccggtt catgcgctcg gcgatgtacg tgaacgcctc
     6841 gtcccagctc gccgtcttcc acgcttcctt gccgcgctca ccggcacgga tcagtggttt
     6901 ctgcagccga tcggggtcgt aatacgagcc gaccgcgccc gttccgcgcg ggcacaagcg
     6961 gccgcggctt tgcggatcga tcggattgcc ttcgaacttc cacagcttgc cgccgcgcac
     7021 gtaggcgatg ccgccgcagc gccagaagca catctcgcag aacgtcggga cggtctcgac
     7081 gcccgcgccc gccggcaggt cggcggcgat cgctcgggcc aacggcggaa tctgcgtggt
     7141 ggttgccgcg gcgccgaagg tcgcggcgga gatcttcaga aagcttcgac gggataagga
     7201 gttgctcatt gcgttgggcc ggaagatgca tccatgcggc cggttgcctg agcgactcga
     7261 ctcgcagcgg actgcaccct ttcctgcggc gctgcggttc atccaggcga acgatcgtcc
     7321 aagtcccgca agccacgttg cggcgcatca atgtcgcggc cgccactgtg atacgcgtca
     7381 cagtcggcgt ggccggggat tcaggcgaca ggcaggcgcg gcacttcgac gcgatgtcgg
     7441 gggcacgcga gtttctcgcg cgcattgagc gcgtgcgctg cacggcgatc ggccgcgcgc
     7501 aatgtggcgc cacgctaagg cttgatgtag gcgtagcctt cgcgcgcctg cagtcgtgcg
     7561 atttcggcca ccgcagcctg cacgacaccc gcctcctctg tcaccgccgc atcggtcagc
     7621 ttgcgcgccg tgagcgtgtt gcgacagacc ttgaacgaga cgccctggcg agccagttcc
     7681 tcgagcgcgg gcgcgaacgg catgccgttc ttgtcgatcg catccttgag caggaattcg
     7741 atcccggcgc cgttggcgat caccacgatc ttcgcggtgg gatcgtcgcg caggtggttg
     7801 cgcacgttcg cgagcaatcc gcgcgctttt tcgatttcgt tgatgtgata gacggtgcgg
     7861 atttctccgc ctgtcgccgc agcaggtggc gcagcagccg gcaagccaac caccagtgcg
     7921 gcagcgagcg aaagtgcaag catgaagcgc ttcatcgtca gggtcccgtt cgatgcgaaa
     7981 gtctgaagac gcgtcgacga tacggcgctc gaagcgcgct ctcttggctc aactcaagcg
     8041 tcgccggggc cggtcggagg aatcctccat cttgttattt cggcgcaaga tcgagcggcc
     8101 catcggcgcg ctctcgcgtc gtcgcacgat cgaatcgggt gcggtcgttg gcacgcaatc
     8161 cgagacgcca cgtgctgcca cgatgagccg gtgaggcgca ctgaaagtga ccactagctt
     8221 atttgccgag gtccaccatc ctttcggtac cgcaaatgct cccgacgtct tcctgcaggt
     8281 caggcgtcga cccgatcggg gtctgcggtc gaagtcgtgc cgcgtggata atcgcgcgca
     8341 tggatgcgac tcgcctgctg ttcgccgtcc tgctggccgt ttccgcggcc gcacccgcgc
     8401 aggagcggcg caactggttc aacgatccat tcgaacgagc cactgctggc ctgccgggct
     8461 gtccggtacc gcaggggccg ctgctgaccg aggcagagca gaagcgcgag gcacactacc
     8521 ggatcgaacg tggaacgacg tgctggctgg cgaagaagtg cgacgagccc aacgcctaca
     8581 agcgcgaccc ggagatcaac gccgccgccg ccgcggcaat ccgcgccgat ccgacgctgc
     8641 gagacaccag cgtctgggtc atcacgcagc gcaagttcgt gttcgtgcag ggctgcgtgc
     8701 gcgcgcgcgc tcagatcaag cgaatcgccg ccgccgtgag agccgacccg cgcgtcgaat
     8761 acgtgggcga cgaggtgctg atcggaacgc gcggcaagcc accgtacccg accacgggct
     8821 cgaaatgagc gggcggcacg gcgcgcgaca aacgctgcgg gcgtcgagca cgggcctgac
     8881 tgcgcgcgaa ctcgccgtac tgcgtcgcct cggcacaccg gcgaagatcc agaccttcct
     8941 gaaccgcatt cccatcaacc acgagcacga cggcgagacc gtgctgtcgg tgcgcgaagt
     9001 gctgcgccag cgccgcgcgc actgtatcga gggcgcgttc gtcgccgcga ccgcgctgtg
     9061 gctgcacggc gagccgccgc tcgtgatgca catggactgc gacctgtcgg actatccgca
     9121 cgtgatcgcc gtctaccggc gcgacggctg ctggggcgcg atttcgaaga ccaacggctc
     9181 gcggctgcgc ttccgcgatc ccgtgtaccg cacgctgcgc gaactggcgc tgtcgtactt
     9241 ccatgaatac acgaacaagc gcggcgagaa gacactgcgc agctacaccg ccgcgttcga
     9301 tctgcgccgg atcgaccctg cgctgtgggt caccaatccg gcgtcgtgct gggaagcgca
     9361 cgaccgcctc gaggcgctgc gccactatcc gttgctggcg ccgcggcaga tccaagcgct
     9421 ggcgcgctcg gatccgttcg agcgccgcgt cggcaaggtc gagcagtacc cggcgccgac
     9481 cagtctggcc cggcgcggat gaacgtcaga actcatactc gaactgcgcg cgcagcgcgc
     9541 gatccggact gctgcgggtg gtgccgaaca gataggcggc gttccatttc agcgcatggc
     9601 cgggcgcgag acgcgtcctg ccgaacagcg ccgggccaat tcggtgcgac tgctgcgacg
     9661 cagggctcca gtgccgccac gggccgaagt cgccgaacgc ctgcgcgccg aattccagcg
     9721 cctcacgcca gcggtacttc gcctgcagcc ggtagccgag ctcggtcgcg gccggctctt
     9781 cgcccctgat ccggcgcttc cagagcagat tcgcgttgta ctgcatgcgc ccggcctcgg
     9841 cctgcagcag cgggccccac gcgaactccc agccttcggc gcgatcgcgt ggccattcga
     9901 tctcgaacag gaggccgagg tcgaagggga acttgccggt ctccgtgagc tggaaacgat
     9961 tctccagttc gacggcgtcg aaatgcgtcg cggcgccttc gcgctcgtac ttcagttcga
    10021 gttcgaccgc ccaccacgac agcgcgccgt agccgatgcc gagcgtggcg gcgctgaagc
    10081 gcggctcgtc cttcttcgtc tgcgtgccga ccttcaactc aaactcgcgc tccccctgat
    10141 caacctgcgg cgacagcacg tcgtcggccg gatcggcacg ggccacggaa acgaggcaca
    10201 agaaggctcc tgccgcggcg gcggcatgca tcttctgcat cttctctcct aggacgtgcg
    10261 gggacgggga acgggcggcg cggtcagcgc gcgcgcggcg aag
//
LOCUS       DBNHBY010000364         2271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_630465, whole genome shotgun sequence.
ACCESSION   DBNHBY010000364 DBNHBY010000000
VERSION     DBNHBY010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_630465"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16HB_09070"
     CDS             <1..411
                     /locus_tag="AC16HB_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HIR5606980.1"
                     /translation="IEPESEGYRERTLRGDYVVRHVVALDPGKGTVAVATEVEAGRQL
                     SFCTRDETAARKDLIRICAEIREHVEEHGGGARGAVYVSCIGRGSHMFGEQSEELRVI
                     RHHLGDVPLVGFYASGEIGGRNLYGFTGVLTVFY"
     gene            complement(427..1221)
                     /locus_tag="AC16HB_09075"
     CDS             complement(427..1221)
                     /locus_tag="AC16HB_09075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5606981.1"
                     /translation="MKLQDRVTIITGAAQGIGAACARAFAAEGAQVVVSDLNEVGGTA
                     LVKDIAAAGGTALFCRADVAVRDDADRLVRFAVDRFGRLDVLVSNAGIVHAADFVALA
                     EEDFDRVLRVNLKGPFLCGQAAARQMLTQSSRTLSGGGSTRGVIINMSSVNAVLAIAN
                     QVPYTVSKGGLNQLTKVMAIGLAPQGIRVMGIGPGSIATEMLKTAVLTNEAARQRILS
                     RTPLGRLGEPDEVARIAVFLASDDASYLTGTTVYPDGGRLGLNYVM"
     gene            complement(1276..2226)
                     /locus_tag="AC16HB_09080"
     CDS             complement(1276..2226)
                     /locus_tag="AC16HB_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5606982.1"
                     /translation="MTKQVNNPRENAEASSGLSIAAVERDSGIAKDTLRAWERRYGFP
                     VPERDEFDERVYPLDQVARLREIKRLLDHGHRPGKIVPMPLHALHALAQAADRGARDA
                     EPASQFELQACLELIRTHRTQELQQWLSRTLAHRGLARFVLEVVAPLNELVGDAWARA
                     ELQIFEEHLYTEAIQLLLRNAIHALPVPVGAPHVLLTTMPPEAHGLGLLMAHALLVLE
                     GARVTSLGVQTPLLDINNAARAHRVQVVALSFSSLQNTSAALEALAQLRAALPATTAI
                     WAGGSCPILHRKPVAGVMTMRALSDIGPALATWRNEQTAS"
BASE COUNT          382 a          772 c          748 g          369 t
ORIGIN      
        1 atcgagcccg aatccgaggg ttatcgcgag cgcacgctcc ggggcgacta tgtggtccgc
       61 cacgtggtcg cgctcgatcc gggcaagggc acggtcgcgg tcgcgaccga ggtcgaggcg
      121 ggccggcaac tgagcttctg cacgcgcgac gagaccgcgg cgcgcaagga cctgatccgc
      181 atctgtgccg agatccgtga gcacgtcgag gagcatggcg gcggcgcgcg cggtgcggtc
      241 tacgtatcgt gcatcggccg cggcagccac atgttcggcg agcagtcgga ggaactgcgc
      301 gtgatccgcc accacctcgg cgacgtgccg ctggtggggt tctacgcgag cggcgagatc
      361 ggcggccgca atctgtacgg cttcaccggc gtgctgaccg ttttctactg aaccgcagtc
      421 gcgagttcac atcacgtaat tcaggcccag ccggccgcca tcgggataga ccgtcgtgcc
      481 ggtcagatac gacgcgtcgt cgctggcgag gaaaacggcg atccgcgcga cttcatcggg
      541 ctcgccgaga cgacccagcg gcgtgcgtga caggatgcgt tgccgggccg cctcgttggt
      601 cagcacggcg gtcttcagca tctcggtggc aatcgagccc gggccgatcc ccatcacacg
      661 gatgccctgc ggcgccagcc cgatcgccat taccttcgtc aactgattca gcccgccctt
      721 gctgacggta tatggcacct ggttggcgat cgcgagcacg gcgttcacac tcgacatgtt
      781 gatgatcacg ccgcgcgtgc tgccgccgcc agacagggtt cgcgaggact gcgtcagcat
      841 ctgccgcgcc gccgcctgac cgcacaggaa cgggcccttc aggttcacgc gcagcacgcg
      901 atcgaaatcc tcctcggcca gcgcgacgaa gtccgccgcg tgcacgatgc cggcgttgct
      961 gaccagcacg tcgagccggc cgaaacgatc caccgcgaat cggaccagcc ggtcggcgtc
     1021 atcgcgaacc gccacgtccg cacggcagaa gagcgccgtg ccgccggccg ccgcgatgtc
     1081 cttgacgagc gccgtgccgc cgacctcgtt caggtcagat acgacaacct gcgcgccctc
     1141 ggccgcgaat gcgcgcgcgc acgcggcgcc gatgccttgc gccgcgccgg tgatgatggt
     1201 gacccgatcc tgaagtttca tcgccgcctc ctccgtcatg ccgcgggcga attgtcgggc
     1261 aaatccgcga ccaggctagc ttgccgtttg ttcgttgcgc caggtggcga gcgcgggacc
     1321 gatgtcggaa agcgcgcgca tcgtcatgac gcccgcaacc ggcttgcgat gcaggatcgg
     1381 gcaactgccg cccgcccaga tcgcggtcgt ggcaggcagc gcggcgcgca gttgcgcaag
     1441 cgcctcgagc gccgcgctgg tgttctgcag ggacgagaac gacagcgcaa cgacctgcac
     1501 gcgatgcgcg cgcgccgcat tgttgatatc gagcagcggc gtctgcacgc ccagcgaggt
     1561 cacgcgcgcg ccctcgagca cgagcagcgc gtgcgccatc agcaaaccca gtccatgcgc
     1621 ctcgggaggc atcgtcgtca gcagcacgtg gggggcgccc accggcaccg gcagcgcatg
     1681 gatcgcattg cgcagcagaa gctggatcgc ctcggtgtac agatgctcct cgaagatctg
     1741 cagttcagcg cgcgcccacg cgtcgccgac caactcgttc agcggcgcga ccacttcgag
     1801 cacgaaacgc gcaaggccgc ggtgtgcgag cgtgcgcgat agccattgct gaagctcctg
     1861 agtccggtgc gtccggatga gttcgagaca ggcctgcagt tcgaactgcg acgcgggttc
     1921 ggcatcgcgc gcgccacggt ctgcggcctg cgccaacgca tgcagcgcgt gaagcggcat
     1981 gggcacgatc ttgccgggcc gatggccatg atccagcagc cgcttgatct ctcgtagccg
     2041 cgccacttgg tcgagcggat agacgcgctc atcgaattcg tcgcgctcag gcaccggaaa
     2101 gccgtagcgg cgctcccatg cccgcagcgt gtccttcgct atcccgctgt cgcgctcgac
     2161 cgccgcgatc gaaagtccgg acgacgcttc ggcgttttcc cttggattgt tgacttgctt
     2221 tgtcatggac aaatagtgta atctacaatt tgtcttggac aaaacaatcg t
//
LOCUS       DBNHBY010000365         2363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_698099, whole genome shotgun sequence.
ACCESSION   DBNHBY010000365 DBNHBY010000000
VERSION     DBNHBY010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_698099"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1007)
                     /locus_tag="AC16HB_09085"
     CDS             complement(9..1007)
                     /locus_tag="AC16HB_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606983.1"
                     /translation="MTDVLLEVADLRKYYPTQQGWFGRGDAKVHAVDGASFSIASGET
                     LGLVGESGCGKSTTGKLILRLQDPTSGTIRWRGRDISRLTQHEMRPVRRELQAVFQDP
                     YASLNPRMRAADIVVEPLRNFEPMSDSAARERAASLFARVGLRADQLVKYPFEFSGGQ
                     RQRLGIARALSVQPQLIVCDEPVSALDVSVQAQVINLLMDLQREFHLSYLFVAHDLAV
                     VEHISHRVAVMYLGKIVEIADKKSIFTRPQHPYTEALLEAVPVPDPKRRKQRRVLTGD
                     VPSPVNPPPGCRFHTRCPYAEARCKVEEPPLKQVAAGQWVACHLRQPVAAKEEAVA"
     gene            complement(1004..2032)
                     /locus_tag="AC16HB_09090"
     CDS             complement(1004..2032)
                     /locus_tag="AC16HB_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5606984.1"
                     /translation="MPQPLLRVENLRTHFHTRDGVVRAVDGVSFDVMPGETLAIVGES
                     GCGKSVTSMSILRLLPMPPARIAEGRIEFDGVDLLQLSEPEMRAIRGNKIAMIFQEPM
                     TSLNPVLTVGRQIAEALVLHRGLSESDATARAIELLRKVHIPEAERRVKQYPHELSGG
                     MRQRVMIAMALACEPRLLIADEPTTALDVTIQAQILELMRELRDSRGKSAGASIILIT
                     HDLGVVAEMAHRVVVMYAGRKVEEAPIEELFARPRHPYTRGLLGSMPHLGDSVNETGK
                     RLVEIPGMVPSLKEPMPGCLFAPRCPNASERCSREVPPLESHGPGHWAACWHPVTIEA
                     KPVAEQVA"
     gene            complement(2235..>2363)
                     /locus_tag="AC16HB_09095"
     CDS             complement(2235..>2363)
                     /locus_tag="AC16HB_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5606985.1"
                     /translation="LIQVAGTILLFPGIFLSLTVLSVNLLGDGMRDALDPRLARRM"
BASE COUNT          380 a          830 c          777 g          376 t
ORIGIN      
        1 ctcgcgcgtc atgcgacggc ctcctctttc gcagcaaccg gctgccgcag gtgacacgcg
       61 acccactgtc cggccgcaac ctgcttgagc ggcggctcct cgaccttgca gcgcgcctcc
      121 gcgtacgggc agcgcgtgtg gaagcggcag cccggcggcg gattgacggg gctcggcacg
      181 tcgccggtca gcacgcgccg ctgcttgcgc cgcttggggt cggggaccgg caccgcttcc
      241 agcagcgcct cggtgtacgg gtgctgcggc cgcgtgaaga tcgatttctt gtcggcgatc
      301 tcgacgatct tgccgaggta catcaccgcg acgcgatggc tgatgtgctc gaccacggcc
      361 aggtcatgcg ccacgaacaa gtacgacaga tggaactcgc gctgcaagtc catcagcaga
      421 ttgatcacct gcgcctgcac cgacacgtcg agcgcggaca ccggctcgtc gcagacgatc
      481 aactgcggct gcaccgaaag agctctcgcg atgccgagcc gctgccgctg cccgcccgag
      541 aactcgaacg gatacttgac caactggtcg gcgcgcaggc cgacccgcgc gaacaggctc
      601 gccgcgcgct cgcgcgcagc gctgtccgac atcggctcga agttgcgcag cggctcgacc
      661 acgatgtcgg cggctcgcat gcgcgggttc agcgacgcat aggggtcctg gaacaccgcc
      721 tgcagctcgc gccgcaccgg gcgcatttcg tgctgagtca ggcgactgat gtcgcgcccg
      781 cgccagcgga tcgtgccgct ggtggggtcc tgcagccgca ggatcagctt gccggtggtc
      841 gacttgccgc agcccgactc gccgacgagc ccgagcgttt cgccactggc gatcgaaaag
      901 ctcgcgccgt cgaccgcatg caccttcgca tcgccgcggc cgaaccagcc ctgctgcgtc
      961 gggtagtact tgcgcaggtc ggcgacttcc agcagcacgt ccgtcatgcg acctgctccg
     1021 caacgggctt cgcttcgatc gtcaccgggt gccagcatgc ggcccagtgt cccggtccgt
     1081 gactttccag cggcggcact tcgcggctgc agcgctcgct cgcgttcggg cagcgcggcg
     1141 cgaacaggca gcccggcatc ggctccttca gcgacggcac catccccgga atctcgacca
     1201 gccgcttgcc ggtctcgttc acggaatcgc cgaggtgcgg catcgagccg agcagcccgc
     1261 gcgtgtatgg atggcgcggg cgcgcgaaca gctcctcgat cggcgcctcc tcgaccttgc
     1321 gacccgcgta catcacgacc acgcggtgcg ccatctccgc gaccacgccc agatcatgcg
     1381 tgataaggat gatcgacgcg ccggcgctct tgcccctgct gtcgcgcagt tcgcgcatca
     1441 gctccaggat ctgcgcctgg atggtcacgt cgagcgcggt ggtcggctcg tctgcgatca
     1501 gcaggcgcgg ctcgcaggcg agtgccatcg cgatcatcac gcgctggcgc atgccgcccg
     1561 agagttcatg cggatactgc ttcacacgcc gctcggcctc gggaatgtgc accttgcgca
     1621 gcagttcgat cgcgcgcgcg gtggcgtcgg actcggacag cccccgatgc agcaccagcg
     1681 cctcggcgat ctggcggccg acggtcagca ccggattgag cgacgtcatc ggttcctgga
     1741 agatcatcgc gatcttgttg ccgcggatcg cgcgcatttc cggttcggaa agctgcagca
     1801 ggtcgacgcc gtcgaactcg atcctgccct cggcgatgcg cgctggcggc atcggcagca
     1861 ggcgcaggat cgacatcgac gtcacgctct tgccgcaacc ggattcgccg acgatcgcca
     1921 gcgtctcgcc cggcatcacg tcgaacgaca ccccgtcgac cgcgcgcaca acgccgtcgc
     1981 gcgtgtggaa gtgcgtcctc aggttctcga cgcgcagcag gggctggggc attacctcac
     2041 catttcatat gccggctggg ggagcacgag ggggcagagc cggggtttcg cgaagcgaac
     2101 gcttcgcacc ggcgaagcgg cgagcgcctg caagcgcgag actggaaccg ccgagccccc
     2161 tcgttccctc gaaggcgctt gcgcgacgtg cgggtcgaag gcgagatcgt taatcatgct
     2221 cgttgaatcc aaagctacat ccgccttgca agccgcggat ccagcgcgtc gcgcatgccg
     2281 tcgccgagca gattcaccga cagcacggtc agcgacagga agatgccggg gaagagcagg
     2341 atcgtgccgg caacctggat cag
//
LOCUS       DBNHBY010000366         7027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1848655, whole genome shotgun sequence.
ACCESSION   DBNHBY010000366 DBNHBY010000000
VERSION     DBNHBY010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1848655"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="AC16HB_09100"
     CDS             <1..776
                     /locus_tag="AC16HB_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR5606986.1"
                     /translation="DAVAAALTVGHLLECSGQISGGNLTEPGVPDLSAEDLSRLGYPV
                     ADIARDGSATIRLLDGQPGRLNVASCTLQLLYEVHDPRAYFTPDAVLDFTSIHFEQQA
                     VNRVRVAGTRGAPRPERLKVIGFLARRGQIADIEIAYAGTGALARARLAADILRLRLP
                     RELAIHESVIDVVGVDSVLGAGSLPLRGEPPEVRVHVSARCEDAELAQAVEDEVYTLT
                     ISGPAGGGSVRSERRGRVESITGFLPRAAVPTRIQWERA"
     gene            773..1084
                     /locus_tag="AC16HB_09105"
     CDS             773..1084
                     /locus_tag="AC16HB_09105"
                     /inference="COORDINATES: protein motif:HMM:NF049520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtuA-related protein"
                     /protein_id="HIR5606987.1"
                     /translation="MRVGDMASGRAGDKADVLDLTLVAYNERDYERLARLLTAERAAQ
                     ILSRVVPGSVVRYELPALRALKFVAPNALPGGVHASLRAGLHWQKAAIWLLLDLPLDA
                     L"
     gene            1087..1581
                     /locus_tag="AC16HB_09110"
                     /pseudo
     CDS             1087..1581
                     /locus_tag="AC16HB_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-keto acid cleavage family enzyme"
     gene            complement(1591..4047)
                     /locus_tag="AC16HB_09115"
     CDS             complement(1591..4047)
                     /locus_tag="AC16HB_09115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5606988.1"
                     /translation="MDQLVHPKVLATAAAPTRFVVPVHGMTCASCVAHVEKALSNVAG
                     VNAVAVNLATESAAVETPPLDAAGRRSTLAAMRSAIADAGYEVPTQTLQLAIEGMTCA
                     SCVARVERALADVPGVLAASVNLASESATVEFVSGAATVRDLVRAVSDAGYEARAPQA
                     DAAPAIDRLGALKRDAILALVLSAPLAAPMLLGLAGIHVALPAWLQWLLATPVQFWCA
                     RRFYVAAAKALRARTGNMDLLVSLGTLAAYGLSLYLWLDEGRSGHLYFEAAAVVVALV
                     LLGRWLEARAKRQTSEAIRLLGELRPSSARVLRDGIEQTVALEEVRLGDIVVALPGER
                     IAVDGQVRAGLTAIDESMLTGEALPVDKQPGDKVTGGALNTTGRIEIETTAVGAETVL
                     ARIIRLVETAQAAKAPIQRLVDRVAAVFVPVVVAIAAATVIGWLFAGATFETALIHAV
                     SVLVIACPCALGLATPTAIIAGTGVAARNGILIRDAEALEAARSVRVVAFDKTGTLTE
                     GKPRVVAAHGHDLLDGREVLALAAAVSAGSTHPLATAVHAALDEHGEITHRRATEHKT
                     LAGRGASARLLDDQGRPTQVELLFGNRRLMQETGLAIDATAQHAQAFEAEGRTVSWLA
                     ERTPDGAHLLGLVAFGDEPKPGARAAVQRLNRMGIETTMLSGDSEAAAAHVAHALGLT
                     RFEAGVLPREKSARVQALKEGGRTVAMVGDGINDAPALAAADVGIAMGSGTDVAIEAA
                     GITLMRGDPRLVADAIEISRATVRKIRQNLFWAFIYNVVGIPLAALGMLSPVIAGAAM
                     AFSSVSVVTNALLLKRYRPH"
     gene            complement(4116..4622)
                     /locus_tag="AC16HB_09120"
     CDS             complement(4116..4622)
                     /locus_tag="AC16HB_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HIR5606989.1"
                     /translation="MKVTAHSITVPVFVRTLTNLSGILDKAAAFCEQRKVREGVLMDA
                     RLFPDMFPLTMQVRIACDFARGGVARLTGQEPPKFEDDETSLSDLKKRIARSIEYVQG
                     FTEAQLAGCEDRRIVRTVGGREIVFDGYGYLAQFVLPNFFFHVTTAYDILRSNGVELG
                     KRDYIGAL"
     gene            complement(4636..5100)
                     /locus_tag="AC16HB_09125"
     CDS             complement(4636..5100)
                     /locus_tag="AC16HB_09125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR5606990.1"
                     /translation="MSETRKPKVIVRNSRIHGRGVYAGRRIRKGERVIEYKGERITWR
                     QADRRPPSDPDDPHHTFFFSLDDGKHVIDANVGGNAARWINHSCDPNCETTEEDGRVY
                     IEAVRDIGAGEELFYDYALIIDERITPTLKKQYKCLCGARDCRGTMLALRKR"
     gene            complement(5156..6019)
                     /locus_tag="AC16HB_09130"
     CDS             complement(5156..6019)
                     /locus_tag="AC16HB_09130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR5606991.1"
                     /translation="MSPALLTVVETAMRAAGAGPLRAARELGRGSFGATLRVDAGAAH
                     YFLKLGTATHPFDAEAQALAAIAATRTLRAPRPLAHGVSAGQGYLLLEWIDLADDGDW
                     HAAGERLAALHAVEASEFGWPRDNTIGASPQFNAPCAGWAEFWRERRLRPQFALARAN
                     GLSELAVLEQRACAASDALLTGHAPAASLLHGDLWRGNLAFDRHGAPVVFDPASYHGD
                     AETDLAMTRLFGGFPATFFAAYSGARPPRAGVEPRQRLYQLYHVLNHANLFGGGYVAQ
                     ARALIEALAAA"
     gene            complement(6021..>7027)
                     /locus_tag="AC16HB_09135"
     CDS             complement(6021..>7027)
                     /locus_tag="AC16HB_09135"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR5606992.1"
                     /translation="RIFPAYYELPLTLAALAALAIWLAWDVKALIGPAVVASIATSYY
                     GLSYRAFLQDDVLLMKRNFYGMLRIRAQGSGDLEVRRLLHGVILHGEQFARGPHRLEP
                     GTYYARTSGVGVAIEDKQKRGPVRIGVVGLGVGTLAAYGRPGDVVHFYELDPDVLDLA
                     RNRFWYLRETRATLEFALGDARLSMERELKEGRPQRFDVLAIDAFASDSIPVHLITRE
                     AIEIYARQIAPDGIIAVHISNRFLDLKPVLANIGAATGLAVRLVSDAPSDESGASSTD
                     WVLIAHDESVFDAPALADRVEEIDPHPEFSLWTDQFNNLLDVLKRRPLSELKSLLFRD
                     "
BASE COUNT         1063 a         2463 c         2425 g         1076 t
ORIGIN      
        1 gcgatgcggt cgcggcagcg ctcaccgtcg gccacctgct cgaatgcagc ggccagattt
       61 ccggtggcaa tctgaccgag cccggcgttc ccgatctctc cgcggaggat ctgtcacgcc
      121 tcggctatcc ggtcgccgac atcgcgcgcg acggaagcgc aacgatccgc ctcctcgacg
      181 ggcagccggg gcggctgaac gtcgcttcgt gcacgctgca gttgctgtac gaagtgcacg
      241 atccgcgcgc gtacttcacg cccgacgcgg ttctcgactt cacgtcgatc catttcgagc
      301 agcaggcggt caatcgcgtg cgcgtggccg gcacgcgcgg cgcgccgcga ccggaacggc
      361 tgaaggtgat cgggttcctc gcccgccgcg gacagatcgc cgacatcgag atcgcatacg
      421 cgggaacggg cgcgcttgcg cgcgcacggc ttgcggcgga catcctgcgg ctgcgcctgc
      481 cgcgcgaact cgccatccac gagagcgtga tcgacgtggt cggggtggac tcggtactcg
      541 gcgcgggttc cttgccactg cgcggagaac caccggaggt gcgcgtgcac gtgtccgcgc
      601 gctgcgagga tgcggaactg gcccaggcgg tcgaggacga ggtctacacg ttgacgatct
      661 cgggccccgc aggcggcggc agcgtgcgca gcgagcggcg cggacgggtc gaatcgatca
      721 ccggattcct gccacgcgcg gccgtgccga ccaggatcca atgggagcgc gcatgagagt
      781 cggggacatg gcttccgggc gcgccggcga caaggccgac gtactcgatc tcacgctggt
      841 tgcctacaac gaacgggact acgagcgcct ggcgcgcctg cttaccgccg aacgcgcggc
      901 acagatactc agtcgcgtcg tccccggctc ggtcgtccgt tacgaactgc cggccctacg
      961 cgcactgaaa ttcgtcgcac cgaacgcgct gccgggggga gtgcatgcat cgctgcgcgc
     1021 gggcctgcac tggcagaagg ccgcgatatg gctgctgctc gaccttccgc tcgacgcact
     1081 ctgaacttcc gcaacacgtc cgccgacatc gcgttcatcc tcgggtcgtg cagcaacaac
     1141 ggcacgcgct tcgagatcga gtgctacgac atcagccacc tgtataccgc ggcgcacttc
     1201 ctcgagcgcg gcttggtgaa gccgccgctg ttcatccagt cggtgtttgg tctgcgcggc
     1261 ggcatcggcc cgcacccaga ggacgtgatg cacatgcggc gcacggccga gcgcctgttc
     1321 ggccgcgact atgtgtggtc ggtactcggc gccggccgca accagatgcc ggtggcgacg
     1381 ctctcggcgg cgatcggcgg caacgtgcgc gtggggctgg aggattcgtt gtgggacggt
     1441 cccggcaacc tcgctcgcac caatgcggcg caggtcaagc ggatccgcac catcctcgag
     1501 gcgctgtcgc tcgagatcgc cacgcctgac gatgcgcggc agatcctgca gctgaagggt
     1561 cgcaaggagg tcgcgttctg atggcgccgt tcagtgcggt cggtaccgct tcagcagcag
     1621 cgcgttcgtg acgacgctga ctgacgagaa cgccatcgcc gcgccggcga tcaccggcga
     1681 cagcatcccc aacgcggcca gcggaatgcc gaccacgttg tagataaacg cccagaacag
     1741 gttctgccgg atcttgcgca ccgtggcacg cgagatctcg atcgcatcgg cgaccaggcg
     1801 cggatcgccg cgcatcagcg tgatgcccgc ggcttcgatc gcgacatcgg tgcccgagcc
     1861 catcgcgatg ccgacatcgg ccgcagcgag cgcgggcgcg tcgttgatgc cgtcgccgac
     1921 catcgcaacc gtgcggcctc cttccttcag cgcctgcacg cgcgcgctct tttcccgcgg
     1981 cagcacgccg gcttcgaagc gcgtcagccc gagcgcgtgc gccacgtggg cggcggcggc
     2041 ctcgctgtcg cccgacagca tcgtcgtctc gatgcccatg cggttcaggc gctggacggc
     2101 ggcgcgcgcc ccgggtttcg gctcgtcgcc gaacgcgacg agacccagca ggtgtgcgcc
     2161 gtccggcgtg cgctcggcga gccacgacac ggtgcggccc tcggcctcga atgcctgcgc
     2221 atgttgcgcg gtggcgtcga tcgccagccc cgtttcctgc atcaggcgcc ggttgccgaa
     2281 cagcagttcg acctgtgtcg ggcgcccctg gtcgtcgagc agccgcgcgc tggcgccgcg
     2341 tccggccagc gtcttgtgct cggtcgcacg gcggtgcgtg atctctccgt gctcgtcgag
     2401 tgcagcgtgt accgccgtgg cgagcgggtg cgtcgagccc gcgctgaccg cggccgcgag
     2461 cgcgagcact tcgcggccgt cgagcaggtc gtgcccgtgc gccgcgacca cgcgcggctt
     2521 gccctcggtc agggtgccgg tcttgtcgaa cgcgaccacg cgcacgctgc gcgccgcttc
     2581 gagtgcctcg gcgtcgcgga tcaggatgcc gttgcgtgcg gccactcccg tgccggcaat
     2641 gatcgcggtc ggcgtagcca gcccgagcgc gcatgggcac gcgatcacca gcacgctgac
     2701 ggcatggatc agcgcggttt cgaatgtcgc gccggcgaag agccagccga tcacggtcgc
     2761 ggcggcgatc gcgaccacga ctggcacgaa caccgcggcg acgcggtcga ccagccgctg
     2821 aatcggcgcc ttggcggctt gcgcggtttc gacgagacgg atgatccggg cgagcaccgt
     2881 ctcggcaccg accgcggtgg tctcgatctc gatgcgcccg gtcgtgttca gcgcgccgcc
     2941 ggtgaccttg tcgccgggct gcttgtcgac cggcagcgct tcgccggtca gcatcgactc
     3001 gtcgatcgct gtcaatccgg cacgcacctg cccgtcgacc gcgatgcgct cgcccggcag
     3061 ggcgaccacg atgtcgccga gacgcacttc ctccagtgcc acggtctgct cgatgccgtc
     3121 gcgcagaacg cgcgcgctcg agggccgcag ttcgccgagc agccggatcg cctcggaggt
     3181 ctgccgcttc gcgcgcgcct ccagccagcg ccccagcagc accagcgcga ccacgaccgc
     3241 ggccgcctcg aagtacaggt gtccgctgcg tccttcgtcg agccacaggt acagcgacag
     3301 gccgtacgcg gcgagcgtgc cgagcgacac cagcaggtcc atgttgccgg tgcgcgcgcg
     3361 cagcgccttg gcggcggcga catagaagcg ccgcgcgcac cagaactgca cgggcgtggc
     3421 cagcagccat tgcagccacg ccggcagcgc gacatgaatt cccgccagcc cgagcagcat
     3481 cggtgccgcc agcggagcgg acagcacgag cgcgagaatc gcgtcacgct tcagcgcacc
     3541 gagccggtcg atggccggcg cggcgtccgc ctgcggcgcg cgggcctcgt aaccggcgtc
     3601 gctgacggca cgcacgagat cgcgcacggt cgccgcgccg ctgacgaact cgaccgtcgc
     3661 cgattccgat gcgaggttga cgctggctgc gagcacgccc gggacgtccg caagcgcccg
     3721 ctcgacgcgc gcgacgcaac tggcgcaggt catgccctcg atcgcgagct gcagcgtctg
     3781 ggtcggaacc tcgtagccgg catcggcgat cgcgctgcgc attgccgcca gcgtgctgcg
     3841 ccggcccgcg gcgtccagcg gcggcgtttc taccgcggcc gactcggtcg ccagattgac
     3901 cgcgaccgca ttgacgccgg ccacgttgct cagcgccttc tcgacgtgcg cgacgcacga
     3961 cgcgcatgtc atgccgtgca ccggcaccac gaaccgcgtc ggcgcggcgg cggtggccag
     4021 aaccttggga tgaacgagct gatccatcgg tggtgcgtgc ccgcgtaagc ctagcgcggg
     4081 cggcgcactc gaggcgcgcg gcggtttgac cggcgtcaga gcgcgccgat gtaatcgcgc
     4141 ttgccgagct cgacgccgtt gctgcgcagg atgtcgtacg cggtcgtcac gtggaagaag
     4201 aagttgggca gcacgaactg cgcgagatag ccgtaaccgt cgaacacgat ctcgcggccg
     4261 ccgaccgtgc gcacgatccg gcggtcttcg cagccggcga gctgcgcctc ggtgaagccc
     4321 tgcacgtact cgatcgaccg cgcgatgcgc ttcttcagat ccgacaatga ggtttcgtcg
     4381 tcctcgaact tgggcggctc ctgccccgtc aggcgcgcca cgccgccgcg cgcgaagtcg
     4441 cacgcgatgc ggacctgcat cgtcagcggg aacatgtccg ggaacaggcg cgcatccatc
     4501 agcacgccct cccggacctt gcgctgctcg cagaacgcgg cggccttgtc gaggatgccg
     4561 gacaggttcg tcagcgtccg cacgaaaacg gggacggtga tcgaatgggc ggtgactttc
     4621 atcggggctc ctttcctagc gtttgcgcag cgcgagcatc gtgccgcggc aatcccgggc
     4681 gccgcacagg cacttgtact gcttcttcag tgtgggcgtg atccgctcgt cgatgatcag
     4741 tgcgtagtcg tagaacaatt cctcaccggc gccgatgtcg cgcaccgcct cgatatagac
     4801 gcgcccgtcc tcctcggtcg tctcgcagtt cggatcgcac gagtggttga tccagcgcgc
     4861 cgcgttgccg ccgacgttgg cgtcgatcac gtgcttgccg tcatcgagcg agaagaagaa
     4921 agtgtgatgc ggatcgtccg ggtccgacgg cgggcggcgg tcggcctggc gccacgtgat
     4981 gcgctcgccc ttgtattcga tgacgcgctc gcccttccgg atgcggcggc cggcgtagac
     5041 gcctcggcca tggatgcgcg agttcctgac gatgaccttc ggtttccttg tttcggacat
     5101 gcgaaacgac gacgtgacga cgcaaacgcg caagtatgcc gcaatcctcc gtcgttcacg
     5161 ccgcggccag cgcctcgatg agcgcgcgcg cctgcgcgac gtagccgccg ccgaacaggt
     5221 tggcgtggtt cagcacgtga tagagctgat acagccgctg ccgcggttcg acacccgcac
     5281 gcggcggtcg cgccccggag taggcagcga agaaggttgc tggaaagccg ccgaacagcc
     5341 gcgtcatcgc cagatccgtc tcggcgtcgc cgtgatagct ggccggatcg aacacgaccg
     5401 gcgcgccgtg ccggtcgaac gcaaggttgc cgcgccacag gtcgccatgc agcagcgacg
     5461 cagcaggcgc atggccggtc agaagcgcgt cgctcgcggc acaggcccgc tgttccagca
     5521 ccgcaagctc gctcaggccg ttggcgcgcg ccagcgcgaa ctgcggccgt agccggcgtt
     5581 cgcgccagaa ctcggcccag ccggcgcacg gcgcattgaa ctgcggactg gcgccgatcg
     5641 tgttgtcgcg cggccagccg aattcgctcg cctcgaccgc atgcagcgcg gccagccgct
     5701 cgccggcggc gtgccagtcg ccgtcatcgg ccagatcgat ccattcgagc agaaggtagc
     5761 cctgccctgc ggacactccg tgcgcgagcg gcctcggtgc gcgcagcgtg cgcgtggcgg
     5821 cgatcgcggc aagcgcctgg gcctcggcgt cgaacgggtg cgtcgccgtg ccgagcttca
     5881 ggaagtagtg cgcggcgccg gcatcgacgc gcagcgtggc accgaacgag ccgcggccga
     5941 gttcacgcgc cgcgcgcagt ggtcccgcgc ccgccgcgcg cattgcggtt tcgacgaccg
     6001 tcagcagcgc agggctcaag tcaatcgcgg aacagcagtg acttcagttc cgacagcggc
     6061 cggcgcttca gcacgtcgag caggttgttg aactgatcgg tccacagcga gaactcggga
     6121 tgggggtcga tctcctcgac gcggtcggcc agcgccggcg cgtcgaacac gctttcatcg
     6181 tgcgcgatca gcacccagtc ggtgctcgac gcgccgcttt cgtccgatgg cgcgtccgac
     6241 accagccgca ccgccaggcc ggtggcggcg ccgatattcg ccagcacggg cttcagatcg
     6301 aggaagcggt tggagatgtg cacggcaatg atgccgtcag gcgcgatctg gcgcgcgtag
     6361 atttcgatcg cttccctcgt gatcaggtgc accgggatcg agtcgctggc gaaggcgtcg
     6421 atcgccagca cgtcgaagcg ctgcggccgg ccctccttca gttcccgctc catcgacagg
     6481 cgcgcgtcgc cgagcgcgaa ttcgagcgtg gccctcgttt cgcgcagata ccagaagcgg
     6541 ttgcgggcca gatcgagcac gtcgggatcg agttcgtaaa agtggaccac gtcgccgggg
     6601 cggccgtacg cggcgagcgt gccgacgccg agtccgacga cgccgatccg caccggcccg
     6661 cgcttctgct tgtcttcgat ggcgacgccg acgcccgagg tgcgcgcata gtacgttccc
     6721 ggttcgagcc ggtgcggccc gcgcgcgaac tgctcgccgt gcaggatcac gccgtgcagc
     6781 agccgccgca cctcgaggtc gccgctgccc tgcgcgcgta tgcgcagcat gccgtagaag
     6841 ttgcgcttca tcagcagcac gtcgtcctgc aggaacgcgc ggtacgacag gccgtagtac
     6901 gaggtcgcga tcgatgcgac cacggccggg ccgatcagcg ccttgacgtc ccacgcgagc
     6961 cagatcgcca gcgcggccag cgccgccagc gtcagcggga gctcgtagta ggcggggaag
     7021 atgcgcg
//
LOCUS       DBNHBY010000367         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1848712, whole genome shotgun sequence.
ACCESSION   DBNHBY010000367 DBNHBY010000000
VERSION     DBNHBY010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1848712"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1264)
                     /locus_tag="AC16HB_09140"
     CDS             complement(47..1264)
                     /locus_tag="AC16HB_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5606993.1"
                     /translation="MKRRTVLSMEQALAMPYATLRFAQLGWRVIRIESTPSGDGLPGD
                     PNRYIGARVVDDDRRSYFIAPNVGKEAIALNLKHPDGQALLRRLLVELDVDVFCCNTV
                     PRRYVQLGIDYETLSAARPGLIWAGISAMGPEYPDVPGYDPVIQAMVGYMELTGAADG
                     PPTLSGVPLIDLKAGDEVYANVMLALLDRAETGKGRRIDVSMLQAAASWLITTLPLLD
                     FDCQKSEITRCGNEHRKFIPTNVYPTADGFIYMAIGSDVQWKRLTEIPKFAEVGNAVR
                     VTNEGRHRERDAIHRDMAVVTRRFATAEIAEDFRQATIPHAPIHDIPAVRALPAIASR
                     VTHTRTPNGKDVRMQPLAVDLPGASGELHFPAHYGEHTHAVLREAGVPVAEIERLSRD
                     GIAYDATQRAAHA"
     gene            complement(1261..>2073)
                     /locus_tag="AC16HB_09145"
     CDS             complement(1261..>2073)
                     /locus_tag="AC16HB_09145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5606994.1"
                     /translation="VVNNDTDITPWDVGVHASRTTFIAGNSAIGAARKAKAKILATAA
                     KKFECAEGELDLRGGHVVRAATGEPLTDLARLIRNLHFSDKAELVMTTFYYEPPSQHQ
                     DKGFKGDVSAAYAWASQVVEVEVDCDTGVVKMTRVTGAHDVGRVLNRLGIEGQIEGGV
                     VMGQGYALTENLMVENGQIRNPNFRDYKLVTAPEIPEIDVSFIESMDGEGPQGAKGVG
                     EAPAICIAAATANAIENATGVRIHELPFTPEKVYRALHGQGEKLYWKPWKPE"
BASE COUNT          279 a          721 c          706 g          367 t
ORIGIN      
        1 cgcgacgtgc tgcggcagcg ggcgctccgg cgtgcgcagc agcgggttat gcatgggcag
       61 ccctctgcgt ggcgtcgtac gcgatgccgt cccgcgacag ccgttcgatc tcggcaacgg
      121 gcacgccagc ctcgcgcagc accgcgtgcg tgtgctcgcc gtagtgcgcg gggaagtgca
      181 gctcaccgga tgcgccgggc aggtcgaccg cgagcggctg catccgcacg tccttgccgt
      241 tcggcgtccg cgtgtgcgtg acgcggctcg cgatcgcggg cagggcgcgc acggcgggga
      301 tgtcgtggat cggcgcgtgc gggatcgtcg cttggcggaa gtcctccgcg atctcggcgg
      361 tcgcaaagcg ccgtgtcacc accgccatgt cgcgatggat ggcgtcgcgc tcccgatgcc
      421 ggccctcgtt ggtcacgcgc accgcgttgc cgacctccgc gaatttcgga atctcggtca
      481 gccgcttcca ctgcacgtcg gagccgatcg ccatgtagat gaagccgtcc gcggtcggat
      541 agacattggt ggggatgaac ttgcggtgct cgttgccgca gcgggtgatc tcgctcttct
      601 ggcagtcgaa gtcgagcagg gggagcgtgg tgatcagcca cgaggcggcg gcctgcagca
      661 tcgacacgtc gatccgccgt cctttgccgg tctcggcgcg atccagcagc gccagcatca
      721 cgttcgcgta cacctcgtcg cccgccttca gatcgatcag cggcacgccc gacagcgtcg
      781 gcggcccgtc ggcggcgccc gtgagttcca tgtagccgac catcgcctgg atcaccgggt
      841 cgtagccggg cacgtccggg tactcgggcc ccatcgccga gatgccggcc cagatcagcc
      901 cgggcctggc cgcgctgagc gtttcgtagt cgatgccgag ctgcacatag cggcgcggca
      961 cggtgttgca gcagaacacg tcgacgtcga gttccaccag cagccggcgc agcagcgcct
     1021 gcccgtccgg gtgcttcaga ttgagcgcga tggcttcctt gccgacattg ggggcaatga
     1081 aatagctgcg ccggtcgtcg tcgaccacgc gcgcgccgat gtagcggttc ggatcgcccg
     1141 gcagcccgtc gccgctcggg gtcgactcga tgcggatcac gcgccagccg agctgcgcga
     1201 agcgcagcgt cgcgtacggc atcgcgagcg cctgctccat cgacagcacg gttctgcgct
     1261 tcattcgggc ttccacggct tccagtacag cttctcgccc tggccgtgca gcgcccgata
     1321 caccttttcc ggcgtgaagg gcagctcgtg gatgcgcacg ccggtggcgt tctcgatcgc
     1381 gttcgccgtg gcggcggcga tgcagatcgc cggcgcttcg cccaccccct tcgcgccctg
     1441 cggcccctcg ccgtccatcg actcgatgaa cgacacgtcg atctcgggaa tctccggcgc
     1501 ggtgacgagc ttgtagtcgc ggaagttggg gttgcggatt tggccgttct ccaccatcag
     1561 gttctcggtc aacgcgtagc cctggcccat cacgacgccg ccttcgatct ggccctcgat
     1621 gccgaggcgg ttcagcacgc ggccgacatc gtgcgcgccc gtcacgcgcg tcatcttgac
     1681 gaccccggtg tcgcagtcga cctcgacctc gaccacctgc gaggcccacg catacgcggc
     1741 cgacacgtcg cccttgaagc ccttgtcctg atgctggctc ggcggctcgt agtagaaggt
     1801 ggtcatcacc agctcggcct tgtcgctgaa gtgcaggttg cggatcagcc gcgccagatc
     1861 ggtcagcggc tcgccggtcg cggcgcgcac cacgtggccg ccgcgcaggt cgagctcgcc
     1921 ttccgcgcat tcgaacttct tcgccgccgt ggcgaggatc ttggccttgg ccttgcgcgc
     1981 ggcgccgatc gccgagttgc cggcgatgaa ggtcgtgcgc gacgcgtgca cgccgacgtc
     2041 ccacggcgtg atgtcggtgt cgttgttgac gac
//
LOCUS       DBNHBY010000368         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1307993, whole genome shotgun sequence.
ACCESSION   DBNHBY010000368 DBNHBY010000000
VERSION     DBNHBY010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1307993"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16HB_09150"
     CDS             complement(<1..205)
                     /locus_tag="AC16HB_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5606995.1"
                     /translation="MPTASRTSPQRSPLFVAGLAKGLAALGAFSAEHPAMSLAELAAA
                     TGLTRSSVQRLVFTLESLGYLRRA"
     gene            292..1608
                     /locus_tag="AC16HB_09155"
     CDS             292..1608
                     /locus_tag="AC16HB_09155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5606996.1"
                     /translation="MRDPAVNDPEQQASLWVATAAPAPECGALDAEANADVCVIGGGY
                     TGFSCALALAEQGTDVVVLEGRGIGHGGAGRNGGQVIPGLKHDPDEIRVLFGPADAER
                     VIELAGSAADRTFALIDRHRIACDARPGGWIQPAHSRAALTTITSRAEQWAKRGAKVE
                     ILDRDGTARALGTHFYHGAWIDRRAGSVQPLSYVRGLARAAIEKGVHTYVQSPAQSLR
                     RAGDRWLVATPQGQVRARVVVLATDAYSGVLWPDLQRNFFGLNSVQIATDVLPPGLRE
                     GIIPCDLPVSETRKLVYYYRLSPDGRFVMGGRGNVAGMVPEHVFASLRVVSERLFPQL
                     KGTRWPYRWWGQVGFTLDWLPHLAEPAPGLWSASGYCGRGVAMATTMGGVLANRVLGG
                     ALARSDPALDFPVRPLRRLPLWSLRKPGVASAIMWFRLRETLGVPA"
     gene            1667..2428
                     /locus_tag="AC16HB_09160"
     CDS             1667..2428
                     /locus_tag="AC16HB_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5606997.1"
                     /translation="MSDRSTRWLRALAVGALVLFGSAALAQPTYKVGSTPTGVPFTFM
                     DTKTQTIEGAMVDLITAVGQDQGFKVQVEATPFSALIPSLTSNKIDIISAAMLITAAR
                     KEVIDFSDPVFPYPEGLIVKADDNKTYKSLADLKGETVGAQVGTVYVDFLKKNGEFAE
                     VKTYDSIADIIRDVGLGRLKAGFGDAPIVRYQLSQRKDSGAKVSETYEPKMDGFVGIG
                     VRKGDKELLAKINAGLAKIKSSGKLDEILKKWNLK"
     gene            2524..>3059
                     /locus_tag="AC16HB_09165"
     CDS             2524..>3059
                     /locus_tag="AC16HB_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5606998.1"
                     /translation="MFSNAFIHDAREFLPILLQGCWTTIKLTALALLLSTVIGFALAL
                     MRLSPYAWLRVPATTFITVIRGIPIIVQLFYIYFVFPDIGISLTAFQAGFIGLGIAYS
                     AYQAENFRAGIEAIDHGQIEAAQSLGMRYLLVLRRVIMPQAIRITLPPYGNTMIMMLK
                     DSSIASTITVAELARQGQL"
BASE COUNT          505 a          964 c         1086 g          504 t
ORIGIN      
        1 gcgcgcggcg caggtagccg agcgactcga gcgtgaagac gagtcgctgc accgaactgc
       61 gcgtcaggcc cgtggcggcg gccagttcgg ccaggctcat cgccggatgt tcggcgctga
      121 aggcgccgag tgcggcgagc cccttcgcga gcccggccac gaagaggggc gagcgctgcg
      181 gcgaggtgcg cgaggcggtg ggcattgaca cgtggcgcca tgcgccgata cgctgtatcg
      241 gcaatcgagt gaagtgtatc gataatcgat acatacggaa agagagattc gatgcgcgat
      301 cccgccgtga acgatccgga acagcaggcg tccctgtggg tggccaccgc ggcgccggca
      361 ccggaatgcg gcgcgctgga tgccgaggcg aatgcagatg tgtgcgtgat cggcggcggc
      421 tacacggggt tttcgtgcgc gctcgcgctg gcggagcagg gcaccgatgt cgttgtgctg
      481 gaggggcgcg gcatcggcca tggcggtgcc ggccgcaacg gcggacaagt gattccgggg
      541 ctgaagcacg atccggacga gattcgcgtc ttgttcgggc cggcggatgc ggagcgcgtc
      601 attgagctgg cgggcagtgc ggcggaccgc acctttgcgc tgatcgaccg ccatcgcatc
      661 gcatgcgatg cccgcccggg cggctggatc cagcccgcac attcgcgcgc ggcactgacg
      721 acgatcacga gccgcgcgga gcaatgggcg aaacgcggcg cgaaggtcga gatcctcgac
      781 cgtgacggga cggcgcgggc gctgggcacg cacttctatc acggcgcatg gatcgaccgg
      841 cgcgccggtt cggtgcagcc gctgtcgtac gtgcgcggac tggcacgcgc ggcgatcgag
      901 aagggggtgc acacgtacgt ccagtccccg gcgcaatcgc tgcggcgcgc aggcgatcgc
      961 tggctggtcg cgacgccgca ggggcaggtg cgcgcgcgcg tggtggtgct ggcaaccgac
     1021 gcctactccg gggtgctgtg gccggatctg cagcgcaatt tcttcggcct gaacagcgta
     1081 cagatcgcga ccgacgtgct gccgcccggt ttgcgcgagg gcatcatccc gtgcgatctg
     1141 ccggtctcgg agacgcgcaa gctcgtctac tactatcgcc tgagtcccga cggacgcttc
     1201 gtgatgggcg gacgcggcaa tgtcgccggc atggtgcccg aacacgtgtt cgcatcgctg
     1261 cgcgtcgtct cggagcggtt gtttccgcaa ctgaagggaa cccgctggcc gtatcgctgg
     1321 tggggacagg tcggttttac gctcgactgg ctgccgcacc tggccgagcc ggcgccgggc
     1381 ttgtggagcg cgagcggata ctgcggacgc ggcgtggcga tggcgactac gatgggaggg
     1441 gtgctcgcca accgcgtgct tggcggcgcg cttgcgcgca gcgatccggc gctcgacttc
     1501 cctgtgcggc cgctgcggcg cctgccgttg tggtcgctgc gcaagcccgg cgtggcgtct
     1561 gccatcatgt ggttccggct gcgcgagacg cttggcgtgc ccgcctgagc cggcgcggca
     1621 cgaatcgaac aattggtctt atcaacctgc gaggaggaga cacaccatga gcgatcgatc
     1681 gacacgctgg ttgcgcgcac tcgccgtcgg cgcgctggtt cttttcggca gcgcggcact
     1741 cgcgcaaccg acctacaagg tgggttcgac gccgacgggc gtgcccttca ccttcatgga
     1801 caccaagacg cagacgatcg agggcgcgat ggtcgacctg atcaccgcgg tcgggcagga
     1861 ccagggcttc aaggtgcagg tcgaagccac gccgttctcg gcgctgatcc cgtcgctgac
     1921 cagcaacaag atcgacatca tctcggcggc gatgctgatc acggccgcgc gcaaggaggt
     1981 gatcgatttc agcgacccgg tgtttcctta ccccgaaggc ctgatcgtca aggccgacga
     2041 caacaagaca tacaagtcgc tcgcggacct gaagggcgag accgtcggtg cgcaggtcgg
     2101 cacggtgtac gtcgacttcc tgaagaagaa cggcgagttc gctgaggtga agacctacga
     2161 ctcgatcgcg gacatcatcc gcgacgtcgg cctcggccgc ctgaaggcgg ggttcggtga
     2221 tgcgccgatc gtgcgctacc agctttcgca gcgcaaggat tccggcgcca aggtatcgga
     2281 gacctacgag ccgaagatgg acggcttcgt cggcatcggc gtacgcaagg gcgacaagga
     2341 actgctcgcg aagatcaacg cggggctggc caagatcaag tcgagcggca agctcgacga
     2401 gatcctgaag aagtggaatc tgaaatagcc tgcggctgac gaaccacggc gcggcggcac
     2461 cgtcgtgccg ccgcggccgg tggcaacacc gccccgtccc gacgccgccc ggagcgaggc
     2521 aagttgttct ccaacgcctt catccacgac gcccgcgagt tcctgccgat cctgctgcag
     2581 ggctgctgga cgacgatcaa gctcaccgcg ctggcgctgc tgctgtcgac cgtgatcggc
     2641 ttcgcgctcg cgctgatgcg gctgtcgccg tatgcgtggc tgcgtgtgcc ggcgacgacg
     2701 ttcatcacgg tgatccgcgg gattccgatc atcgtgcagc tcttctacat ctacttcgtg
     2761 ttcccggaca tcggtatcag cctgaccgcg ttccaggccg gcttcatcgg cctgggcatc
     2821 gcgtactccg cctaccaggc ggagaacttc cgtgccggga tcgaggcgat cgaccatggg
     2881 cagatcgagg cggcgcagtc gctcggcatg cgttacctgc tggtcctgcg gcgcgtgatc
     2941 atgccgcagg cgatccgcat cacgctgccg ccctacggca acacgatgat catgatgctg
     3001 aaggattcgt cgatcgcgtc gacgatcacg gtggcggaac tggcgcgcca ggggcagct
//
LOCUS       DBNHBY010000369         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_766798, whole genome shotgun sequence.
ACCESSION   DBNHBY010000369 DBNHBY010000000
VERSION     DBNHBY010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_766798"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1274
                     /locus_tag="AC16HB_09170"
                     /pseudo
     CDS             27..1274
                     /locus_tag="AC16HB_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
     gene            1271..>2106
                     /locus_tag="AC16HB_09175"
     CDS             1271..>2106
                     /locus_tag="AC16HB_09175"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR5606999.1"
                     /translation="MNWQAALTAMLPEHLVLVGIVLLVLAELGPRRSNALTTISVAAV
                     LVAAIAAAGLALLGYSAAPFAGHFSVNPTTLGAKAIVLALTAPVLLMGRDDFAHDGQF
                     HLLTLSSLYGVSLLLSSDSFLTLFLGLELMSLPVYALVLLAFRRPESAEAALKYLVLG
                     GAATATFLMGVSLLFGASGSLALSTFSKALGSTDLLAQAAVVLVIVSFFLKAAVVPFH
                     TWAPDAYEAASIPVTAYMAVVIKAGVLLAAVRLFAQATLSPAMVDLLAILPLISMVWG
                     NLT"
BASE COUNT          269 a          702 c          713 g          422 t
ORIGIN      
        1 tgaccgcgtt cctgggtccc ctggtcgtgg ccggcgcgtt ctcggcgatc accaaggacg
       61 tcaagctctt ctacgcgatg gtgttcctga tccagttcgc gatgctcggc accttcgtcg
      121 cgcaggacct gttcctcttc tacgtgttct gggaaacgat gctgatcccg atgttcctga
      181 tcatcggcat ctggggcggc gagcggcgca tctacgcgac gctgaagttc ttcctgtaca
      241 ccgcgtttgg ctcgatcctg atgctggcgg cggtgatcta tctcgtgtac tcactgcagc
      301 agaccgccgg cgtggcgtcg tttgccttcg ccgacctgta caaggtccgg ctgccggtcg
      361 acgtgcagaa gatcctgctc gccgcctttg cgctgtcgtt cgcgatcaag gtgccgatgg
      421 tgccgctgca tacgtggctg cccgacgcgc acgtcgaggc gccgaccacc ggctccgtga
      481 tcctggccgg cgtgctgctg aagatgggaa cgtacggttt catgaagctc ggcttcccgc
      541 tcttcccgga cgccacccgc gacgcgacgc cgctgatcat ggcgctcgcg gtgatcagca
      601 tcgtctacgg cgcgtgcctg gcgctggtgc agaccgacat caagaagatc atcgcctact
      661 cgtcgatcag ccacctcggc tacgtgatgc tgggcctggc gagcctggac ctgctcggca
      721 tccagggcgc gatcatccag atggtgagcc acgggctgac ggcagccggg ctcttcctga
      781 tggtcggcat gatctaagaa cgctgccaca cgcgtgaact cgccgcgtac ggcgggctgg
      841 cgaagctcct gccggtgtac tcggtgttct tcgcgatcct gacgctggcg tcgatcgggc
      901 tgccgaccac gagcggcttc accggcgaat tcctggtgct gctcggcgcg ttccgcggcg
      961 cgtggccggc gtacgtggcc ggcgacacgt tcccgctggc gatgagcgtg atcgcggtgt
     1021 cgggcgtggt gctcggggcg ctgtacatgc tgtggttcgc gcagcgcttc ctgttcgggc
     1081 tcgccaaggt gccgcatgcg ccggtgacgg atctgaatcg gcgcgagaaa ctgatcctcg
     1141 tcgcgatcgt cgccggggtg ttctggctcg gtttgtaccc gcaggagccg atgcgcaaga
     1201 ccgaactcgc ggccaaggag taccagcaac tggtcggcac gtcgcgcacg acgctggtgg
     1261 ggagcgcacg atgaactggc aagccgcact gaccgcgatg ctgcccgagc acctggtgct
     1321 cgtcggcatc gtgctgctgg tcctggccga actcgggccg cgccgctcga acgccctgac
     1381 cacgatctcc gtcgcggccg tgctcgtcgc ggcgatcgcg gccgcggggc tggcgctgct
     1441 gggctactcc gccgcgccgt tcgccggtca cttctcggtc aatccgacca cgctcggcgc
     1501 caaggcgatc gtgctcgcgc tcaccgcgcc ggtgctgctg atggggcgcg acgacttcgc
     1561 gcacgacggg cagttccacc tgctgacgct gtcgtcgctg tatggcgtga gcctgctgct
     1621 gtccagcgac agcttcctga ccctattcct ggggctcgag ttgatgtcgc tgccggtgta
     1681 cgcgctggtg ctgctggcgt tccgccgccc cgagagtgcc gaggcggcgc tgaagtacct
     1741 ggtgctcggg ggcgcggcca ccgcgacctt cctgatgggc gtgtcgctgc ttttcggagc
     1801 cagcggctcg ctggcgcttt cgacgttctc caaggcgctc ggctcgaccg acctgctggc
     1861 gcaggccgcc gtggtgctgg tcatcgtgtc gttcttcctg aaggccgcgg tggtgccgtt
     1921 ccacacctgg gcgcccgacg cgtacgaggc cgcgagcatc ccggtgaccg cgtacatggc
     1981 ggtcgtgatc aaggccggtg tgctgctcgc cgccgtgcga ctgttcgcgc aggcgacgct
     2041 ctcgcccgcg atggtcgacc tgctggcgat cctgccgctg atctcgatgg tgtggggcaa
     2101 cctcac
//
LOCUS       DBNHBY010000370         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1510957, whole genome shotgun sequence.
ACCESSION   DBNHBY010000370 DBNHBY010000000
VERSION     DBNHBY010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1510957"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="AC16HB_09180"
     CDS             complement(<1..1364)
                     /locus_tag="AC16HB_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5607000.1"
                     /translation="MTDIITMQVIRYGLEQVADEMGNTLVRTGRSTIITEIKDISCVV
                     TDVRGQTVAQAHHNPSLLAGFEITMRELVAAYPPEQLAPGDVIITNDPYRGGQHIMDL
                     YAIAPAFRGGRLIGFVGNITHHSDLGGVAAGGVAGGVREIYLEGLRLPMVKLYKGGRE
                     DAEIVGIIANQIRVPDKTLGDVRAQVASLMVGTERLGRMFSRHGEETMRAACAELLDY
                     SERRMRHGLAALPAGTYEAEDFIDDDGVNDRPIRIHVKLTIGSGEVVVDLTGCDPQAE
                     GNTNSTLANTHAAAYYVMIAVVDPGIPPNSGCYRPIKVLTRKGSIVDPLPPGAVAART
                     NASQKIVEAMLRALAPAVPDRVTAGSHGQISTCGYGGRQPDTGAPFIYTDIQGGGSGA
                     RPYRDGRDGQDSHLPRFMNTPVEVLERQFPVRIERYEFIRDSGGPGAYRGGLALRRDV
                     RLLA"
     gene            complement(1361..>2236)
                     /locus_tag="AC16HB_09185"
     CDS             complement(1361..>2236)
                     /locus_tag="AC16HB_09185"
                     /inference="COORDINATES: protein motif:HMM:NF039581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5607001.1"
                     /translation="AKRAIGSLAAEMNMGLEETALGIVRVVNANMVKGIAAVTILRGI
                     DVRDFSLLSFGGAGGVHAVDLARALSMREAIVPPLPGTFSAVGLLVTDTRLDYVAALG
                     GVRADQADLAALEAAYRDMEKQAAAELAEQGFGPESCSLLRRADLKVAGQTYELSLPL
                     PHPGPLDAAGLRALLEAFARLYRERYAFFFDGEPIEIVNLRLAALGRNAPIELPRYAR
                     AGAEPAAALKATRPVYFESIGFAPTAVYERDRLRPGMVVPGPALIEEETSSTLVPPGA
                     HADVLDDLGLVIPIQ"
BASE COUNT          326 a          787 c          773 g          350 t
ORIGIN      
        1 tcggcgagca gccgcacgtc gcgccgcagc gccagcccgc cgcgatacgc gccgggtccg
       61 cccgaatcgc ggatgaactc gtagcgctcg atgcgcacgg ggaactgccg ttcgagcact
      121 tcgaccggcg tattcatgaa acgcggcagg tggctgtcct ggccgtcgcg cccgtcgcga
      181 tagggtcggg cacccgagcc gccgccctgg atgtcggtgt agatgaacgg cgcgccggtg
      241 tcgggctgcc gtccgccgta gccgcaggtg ctgatctgtc cgtgcgagcc cgcggtcacg
      301 cgatccggca ccgcgggcgc gagcgcgcgc agcatcgcct cgacgatctt ctgcgatgcg
      361 ttggtgcgcg cagcgaccgc gcccggcggc agcggatcga cgatgctgcc cttgcgcgtc
      421 aataccttga tcggccggta gcagcccgag ttgggcggga tgcccggatc gacgaccgcg
      481 atcatcacgt agtacgcggc cgcgtgcgtg ttggcgagcg tcgagttggt gttgccctcg
      541 gcctgcggat cgcagccggt cagatcgacg acgacttcgc cactgccgat cgtgagcttc
      601 acgtggatgc ggatcgggcg atcgttgacg ccgtcgtcgt cgatgaagtc ctcggcctcg
      661 tacgtgccgg ccggcagcgc ggccagtcca tggcgcatgc gccgctcgga atagtcgagc
      721 agttccgcgc acgccgcgcg catcgtttcc tcgccgtggc ggctgaacat gcggccgagc
      781 cgctcggtgc cgaccatcag cgaggcgacc tgcgcacgga cgtcgccgag cgtcttgtcc
      841 ggcacgcgga tctggttggc gatgatgccg acgatctccg cgtcctcgcg tccgcccttg
      901 tacagcttca ccatcggcag gcgcagccct tcgaggtaga tctcgcgcac gccgcccgcg
      961 acaccgcccg ccgccacgcc gccgaggtcg gagtggtgcg tgatgttgcc gacgaagccg
     1021 atcagcctgc cgccgcggaa cgccggcgcg atcgcgtaca ggtccatgat gtgctggccg
     1081 ccgcggtacg gatcgttggt gatgatcacg tcgcccggcg cgagctgttc gggcggatac
     1141 gcggccacca gctcgcgcat cgtgatctcg aagcccgcca gcaggctcgg attgtggtgc
     1201 gcctgcgcga ccgtctgccc gcgcacgtcg gtgaccacgc acgagatgtc cttgatctcg
     1261 gtgatgatcg tcgagcggcc ggtgcgcacc agcgtgttgc ccatctcgtc cgcgacctgc
     1321 tccaggccgt agcggatcac ctgcatcgtg atgatgtcgg tcattggatt ggaatcacga
     1381 gtccaagatc gtcgagtacg tccgcatgcg cgccgggcgg caccagcgtc gaggaggtct
     1441 cttcctcgat cagcgccgga ccagggacga ccatgccggg ccgcaaccgg tcgcgctcat
     1501 agacggcggt cggcgcaaag ccgatcgatt cgaagtacac cgggcgcgtc gccttcagcg
     1561 ccgctgccgg ttcggcgccc gcgcgcgcgt agcgcggcaa ctcgatcggc gcgttgcggc
     1621 cgagcgcggc gagcctcagg ttgacgatct cgatcggttc gccgtcgaag aagaacgcat
     1681 agcgctcacg gtacaggcgc gcgaaggcct cgagcagcgc gcgcagcccg gccgcatcga
     1741 gcggccccgg gtgcggcagc ggcagcgaca gctcgtacgt ctgcccggcg accttcagat
     1801 ccgcgcggcg gagcaggctg catgactcgg gaccgaagcc ctgctccgcg agttccgccg
     1861 cggcctgctt ctccatgtct cgatacgcgg cttcgagcgc agcgagatcc gcctgatccg
     1921 cgcgcacgcc gccgagtgcg gccacgtagt cgagccgcgt gtcggtgacc agcagcccga
     1981 ccgcggaaaa cgtgcccggc agcggcggca cgatcgcctc gcgcatcgac agggcgcgcg
     2041 cgaggtcgac cgcgtgcacg ccgcccgcgc cgccgaacga cagcagcgag aagtcgcgca
     2101 cgtcgatgcc gcgcaggatc gtcaccgcgg cgatgccctt gaccatgttg gcgttgacca
     2161 cgcgcacgat gccgagtgcg gtttcctcca gccccatgtt catctcggct gcgagcgacc
     2221 cgatcgcgcg cttcgc
//
LOCUS       DBNHBY010000371         8696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_21677, whole genome shotgun sequence.
ACCESSION   DBNHBY010000371 DBNHBY010000000
VERSION     DBNHBY010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_21677"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16HB_09190"
     CDS             complement(<1..250)
                     /locus_tag="AC16HB_09190"
                     /inference="COORDINATES: protein motif:HMM:NF026042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607002.1"
                     /translation="MAAKKKTIRTIPQERTPMPEQDPRERARNFEEVACGYGVADAQR
                     EAERCLQCPDQPCVRGCPVGIDIPAFIQKIADKHYHGAY"
     gene            complement(252..1172)
                     /locus_tag="AC16HB_09195"
     CDS             complement(252..1172)
                     /locus_tag="AC16HB_09195"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR5607003.1"
                     /translation="MAQANTGAAFRIVAREDFSDVTYLLEVAHPMLARAAKPGQFVIV
                     MEHEHGERIPLTIADFDRNKGTITLVIQAVGKTTREMQRNCRAGTTLYAVVGPMGIPS
                     HIGAAKKVVCVGGGLGVAPVYPQARAYKESGAYVIGVIGFRNRDLIFWRDKFSAVCDE
                     LIVCTDDGSAGLKGLVTEGIRQAIARHKDIDEVVAIGPPIMMKGCAEATRPHKIKTMV
                     SLNPVMVDGTGMCGGCRVKIGDQVKFDCVDGPDFDGHQVDFDDLMARLVRYKPSEQNA
                     LLKWQEGCRLQEIAEPESETNSLDRPSTDG"
     gene            complement(1345..2400)
                     /locus_tag="AC16HB_09200"
     CDS             complement(1345..2400)
                     /locus_tag="AC16HB_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR5607004.1"
                     /translation="MTYIAKPVLHHPTLPTNRLGYTRRDYEGKISTLCAGCGHDSISA
                     AIIQACWELAIEPHRVAKLSGIGCSSKTPDYFLGNSHGFNSVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHLIRRGVNMTYICEDNGVYGLTKGQFSATADAGSKS
                     KRGAVNRDAPIDMVSLALQIGVTYVARSFSGDKEQLVPLVKGALNHRGAALIDVISPC
                     VAFNNHAGSTKSYEYVRAHNDAVNRLDFMPKRDAITARYEPGEVIEITQHDGSLLRLR
                     KLADGYDPGDRLAAISHIHQHHARGEIVTGLLFVDPSSDDLHDHLNTVDTPLNRLGEA
                     ELCPGAAALEALNAELT"
     gene            complement(2414..4309)
                     /locus_tag="AC16HB_09205"
     CDS             complement(2414..4309)
                     /locus_tag="AC16HB_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5607005.1"
                     /translation="MNAPDRGGLEIPRLGAPPRRIAAVNDFVIKFANVNGSGSASANE
                     LFARSILRMGVPVSPRNIFPSNIQGLPTWYEVRVTEHGHLGRRGGVDLLVAMNPQTWD
                     KDIAEIEPGGYLFYDSTKPLPPSKFRPDVHVIGVPLTDLAAAKFTDAKERQLLKNIMY
                     VGALSAMLGIEPEVIAQLLGEQYKGKDKLIRPNLEAFMTGLHYGRAHLEDTLALRIER
                     RNAVGDRIFLEGNAAAALGCLYGGATVCAWYPITPSSSLAEAFQKHCARYRVDKATGK
                     NRFAIVQAEDELASIGIVIGAGWNGARAFTATSGPGISLMSEFIGLAYFAEVPAVIID
                     VQRGGPSTGMPTRTQQSDLLACAYASHGDTKHVLLFPEDPHECFEFAAAALDLAERLQ
                     TPIFVMSDLDIGMNHRLCAPLKWDDSRRYDRGKVMSAQQLEASRTFFGRYLDVDSDGI
                     PWRTLPGAHPTKGAYFTRGTTKDAYARYSEAGPDYMYNMERLLKKFSTAKKLVPQPIV
                     RRASQKTRFGVIYFGSTSPAMTEALGALEADGIYLDRLRVRAFPFADSVAQFVAEHDR
                     VFVVEQNRDGQLRSMLVTELEIDPARLVPILHYDGTPITARFIAQAIADRATGTNVRP
                     LKSGKVA"
     gene            complement(4309..6129)
                     /locus_tag="AC16HB_09210"
     CDS             complement(4309..6129)
                     /locus_tag="AC16HB_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5607006.1"
                     /translation="MQATEIRNPDYFHKVGDCQGACPAHTPVPEYIRLIAAGKYSDAY
                     MVNWKSNVFPGILGRTCDRPCEPACRRGRVEQAQTERPEPVAICRLKRVAADLKDDIR
                     ARLPKLIAPRNGRRVACVGAGPASLTVARDLAPLGYEVTVFDQDARSGGMIRSQIPRF
                     RLPEEVIDEETGYVLGLGVDFRAGVRIDSMQHLLAQGFDAVFVGSGAPRGRDLRIPGR
                     TEAAAHIHVGIDWLASVSFGHVEKIGKRVIVLGGGNTAMDCCRTARRLGGEDVKVIVR
                     SGFEEMKASPWEKEDALHEGIPILNYLVPKEFLHRDGRLLGMSFEKVKAVYDANGRRE
                     LVPTGEPDPIFECDEVLIAVGQENAFPWIERQLGIEFDRSGMPVLDKATLQSTLPNVL
                     FGGDAALGPKNIIWAVAHGHEAAISIDKLLHGEDTRQRPAAGVTLLSQKMGIHEWSYD
                     NDVTPDARHKVPWRDVNQVLKNIRVEVELGFDAATAWKEAQRCLNCDVQTVFNAPLCI
                     ECDACVDICPTDCINFTANDAEDALRARLRAPALNRDQALYVSPALKTGRVMVKDEDV
                     CLHCGLCAERCPTGAWDMQKFMLDLPYAGRACRPPQRKAA"
     gene            6458..>8696
                     /locus_tag="AC16HB_09215"
     CDS             6458..>8696
                     /locus_tag="AC16HB_09215"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR5607007.1"
                     /translation="MMRQFQVNSYLFGGNAPYVEELYEKYLDHPASVPEKWRAYFDQL
                     QLVPAADGNPQSPDVAHAPIVESFALRAKTGTLRPTVAPTDLSVARKQVHVQSIVAAY
                     RFLGARWADLDPLKRQERPHIPELEPGFYDLTEADMDIVFSAANTYFGQEQMTLREIV
                     KALRQTYCSTIGVEYMYISDPAEKRWWQQRLESIRSTPTYTAEQKTHILERLTAAEGL
                     ERYLHTKFVGQKRFSLEGGESFIPAMDEIVMHAGEMGVEEIVIGMAHRGRLNVLVNTL
                     GKMPKDLFAEFEGKAASDLPAGDVKYHNGFSSDVTTPGGPVHLSLAFNPSHLEIVNPV
                     VEGSVRARQERRGDNKREKVLPVLVHGDAAFAGQGVVMETLNLAQTRGYKTGGTVHII
                     INNQIGFTTSDPRDSRSTLYCSDVVKMIEAPVLHVNGDDPEAVVLCARFAMEYRQQFK
                     KDVVIDIICFRRLGHNEQDTPAVTQPLMYKKIGSHAGTRRLYADRLVTQGTLRGETPD
                     EMVKAYRAAMDAGRHTVDLVLTNYKSKYAVDWSPFLNRRWTDHADTAVPIAELKRLAE
                     RITTVPANFKLHPLVDKVIADRRAMGEGRVPLDWGMAEHLSFASLVASGYPVRITGQD
                     CGRGTFSHRHAVLHDQNRERWDAGVYVPLQNAAENQAPFLVIDSVLSEEAVLGFEYGY
                     SCAEPNALVIWEAQFGDFVNGAQVVIDQFISSGEVKWGRQSGLTLMLPHGYEGQGPEH
                     SSARIE"
BASE COUNT         1404 a         2858 c         2905 g         1529 t
ORIGIN      
        1 cgtaggcgcc gtggtagtgc ttgtcggcga tcttctggat gaacgccggg atgtcgatgc
       61 cgaccgggca gccgcgcacg cacggctggt ccgggcactg caggcagcgc tcggcctcgc
      121 gctgcgcgtc ggcaacgccg tagccgcatg cgacttcctc gaagttgcgc gcacgctcgc
      181 gcggatcctg ctccggcatc ggcgtgcgct cctgcgggat cgtgcggatc gtcttcttct
      241 tcgctgccat gtcagccatc cgtcgagggt cgatcgagcg agttcgtttc ggactcaggc
      301 tcggcaatct cctgcaggcg gcagccctcc tgccacttga gcagcgcgtt ctgctccgag
      361 ggcttgtagc gcacgaggcg cgccatcaga tcgtcgaagt cgacctgatg gccatcgaag
      421 tcggggccgt cgacgcagtc gaacttcacc tggtcaccga ttttcacgcg gcagccgccg
      481 cacatgccgg tgccgtcgac catgaccggg ttcaggctca ccatggtctt gatcttgtgg
      541 ggtcgcgtgg cctcggcgca gcctttcatc atgatcggcg ggccgatcgc gaccacttcg
      601 tcgatgtcct tgtggcgggc gatcgcctgc ctgatgccct cggtcaccag tcccttcaag
      661 cccgccgacc cgtcgtcggt acagacgatc agttcgtcgc acacggcgct gaacttgtcc
      721 cgccagaaga tcaggtcgcg gttgcgaaag ccgatcacgc cgatcacgta ggcgccgctc
      781 tccttgtagg cgcgcgcctg cggatagacc ggcgcgacgc ccaggccgcc gccgacgcac
      841 acgaccttct tcgccgcacc gatatggctc ggaatcccca ttggcccgac caccgcgtac
      901 agcgtcgtgc cggcacggca gttccgctgc atctcgcgcg tggtcttgcc gaccgcctgg
      961 atcaccagag tgatcgttcc cttgttgcgg tcgaagtcgg cgatcgtcag cgggatgcgc
     1021 tcgccgtgct cgtgctccat cacgatcacg aactggccgg gcttggcggc acgcgccagc
     1081 atcggatgcg ccacctcgag caggtaggtg acgtccgaaa aatcctcgcg cgcaacgatg
     1141 cggaatgcag cgcccgtgtt cgcctgcgcc atacctgtct ccctcgtgtg ggtttccgcg
     1201 ccctcgcacc tgcgaaacgc gcggctatga agcgaacggc ctcacgtagg gcgcagcttc
     1261 gtgcgaccgg aggcagcggg tgttgatctt cggcaagcaa gcccggatgc gcgcagcgag
     1321 cgacgtgcag cggcgacgcg attcctacgt gagctcggcg ttcagtgctt cgagcgcggc
     1381 cgcgccgggg cacagttccg cctcaccgag ccggttcagc ggcgtgtcga cggtgttcag
     1441 atgatcgtgc aggtcgtcgg acgaaggatc gacgaacagc aggccggtca cgatctcgcc
     1501 gcgcgcatga tgctggtgga tgtggctgat cgccgcgagc cggtcgccgg ggtcgtagcc
     1561 gtcggcgagc ttgcgcaggc gcagcaggct gccgtcgtgc tgtgtgatct cgatcacttc
     1621 gcccggttcg tagcgcgcgg tgatcgcgtc gcgcttcggc atgaaatcga gccggttgac
     1681 cgcatcgttg tgcgcccgga cgtattcgta gctcttcgtg ctgccggcgt ggttgttgaa
     1741 cgcgacgcac gggctgatca cgtcgatcag cgccgcgcca cgatggttca acgcgccctt
     1801 gaccagcggc accagctgct ccttgtcgcc ggagaagctg cgcgcgacgt aggtcacgcc
     1861 aatttgcagc gcgagggaca ccatgtcgat cggcgcatcg cggttgactg cgccgcgctt
     1921 ggacttcgag ccggcatcgg cggtggcgga gaactgccct ttggtgaggc cgtagacgcc
     1981 gttgtcctcg cagatgtatg tcatgttgac accgcgccgg atcaggtgcg cgaactgtcc
     2041 cagtccgatc gatgccgagt cgccgtcgcc ggacaccccg agatagatca gctcccggtt
     2101 cgcgaggttc gcaccggtca ggaccgacgg catccgaccg tgcacggagt tgaagccgtg
     2161 cgaattgccg aggaaatagt ccggcgtctt cgacgagcac ccgatgcccg acagcttggc
     2221 gacccggtgc ggctcgatcg ctagctccca gcacgcctgg atgatcgcag ccgagatcga
     2281 gtcatgcccg cagccggcgc acagcgtcga gatcttccct tcgtaatcgc gccgcgtgta
     2341 gccgagccgg ttggtcggca atgtcgggtg gtggaggaca ggcttggcga tgtaagtcat
     2401 ggtgggtggg gcgtcaggcg accttgccgc tcttcagcgg ccgcacgttg gtgccggtgg
     2461 cgcggtcggc gatcgcctgc gcgatgaagc gcgcggtgat cggcgtgcca tcgtagtgca
     2521 ggatcggtac cagccgtgcc ggatcgattt cgagctcggt gaccagcatc gagcgcagct
     2581 gcccatcgcg gttctgctcg acgacgaaca ccctgtcatg ctcggcgacg aactgggcga
     2641 ccgaatcggc gaacgggaag gcacgtacgc gcagacggtc gagataaatg ccgtcggctt
     2701 ccagcgcgcc gagcgcctcc gtcatcgccg gactggtcga gccgaaatag atcacgccga
     2761 agcgcgtctt ctgcgaggcg cggcggacga tcggctgcgg cacgagcttc ttcgccgtcg
     2821 agaacttctt cagcagccgc tccatgttgt acatgtagtc gggccccgcc tcggaatacc
     2881 tcgcgtaggc gtccttggtc gtgccgcgcg tgaagtacgc gcccttggtc gggtgtgcgc
     2941 ccggcagcgt gcgccaaggg attccgtcgc tgtcgacatc gaggtagcgg ccgaagaacg
     3001 tgcggctcgc ttcgagctgc tgggcgctca tcaccttgcc gcggtcgtag cggcgcgaat
     3061 cgtcccactt cagcggggcg cacaggcggt ggttcatgcc gatgtcgagg tcgctcatca
     3121 cgaagatcgg cgtctgcagg cgttcggcca ggtcgagcgc cgccgctgcg aattcgaagc
     3181 attcgtgcgg gtcctcgggg aacagcagca cgtgcttcgt atcgccatgc gacgcgtacg
     3241 cgcaggcgag caggtccgac tgctgcgtgc gcgtcggcat tccggtcgac ggtccgccgc
     3301 gctgcacgtc gatgatcacg gccgggacct cggcgaaata cgcgagtccg atgaactcgc
     3361 tcatcagcga gatgccgggg ccggaggtcg cggtgaaggc ccgcgcgcca ttccagccgg
     3421 cgccgatcac gatgccgatc gacgccagtt cgtcctcggc ctgcacgatt gcgaagcggt
     3481 tcttgccggt cgccttgtcg acgcggtagc gcgcgcagtg tttctggaat gcctcggcca
     3541 gtgacgacga cggcgtgatc gggtaccacg cgcaaaccgt cgccccgccg tacaagcacc
     3601 cgagcgctgc agccgcgttg ccctcgagga agatgcggtc gccgaccgcg ttgcggcgct
     3661 cgatgcgcag cgcgagcgtg tcttcgaggt gcgcgcggcc gtagtgcagg ccggtcatga
     3721 acgcctccag gttcggccgg atcagcttgt ccttgccctt gtactgctcg ccgagcagct
     3781 gcgcgatcac ctcgggctcg atgccgagca tcgcggacag cgcgcccacg tacatgatgt
     3841 tcttcagcag ctggcgctct ttcgcgtcgg tgaacttcgc cgccgccagg tcggtcagcg
     3901 gcacgccgat gacatggacg tcagggcgga atttcgacgg cggcaacggc ttcgtgctgt
     3961 cgtagaacaa gtagccgccg ggctcgatct cggcgatgtc cttgtcccac gtctgcggat
     4021 tcatcgccac cagcagatcg acgccgccgc gccggcccag gtgaccgtgc tcggtgacgc
     4081 gcacctcgta ccacgtgggc aggccctgga tattggatgg aaagatgttg cgcggtgata
     4141 ccggcacgcc catgcgcagg atgctgcgcg cgaacaactc gttcgcgctc gccgagcccg
     4201 agccgttgac gttggcgaac ttgatcacga agtcgttgac ggccgcgatc cgccgcggcg
     4261 gcgcgcccag gcgcgggatc tccagaccgc ctcgatcggg cgcattcatc aggcggcctt
     4321 tcgctgcggc ggccggcatg cgcgccccgc gtacggcaga tcgagcatga acttctgcat
     4381 gtcccatgcg ccggtcgggc accgttccgc gcatagcccg cagtgcaggc acacgtcctc
     4441 gtccttgacc atcacgcggc ccgtcttgag cgcgggtgac acgtacagcg cctggtcacg
     4501 gttcagtgca ggcgcgcgca ggcgcgcacg cagggcgtct tcggcatcgt tggccgtgaa
     4561 gttgatgcag tcggtcggac agatatcgac gcatgcatcg cactcgatgc acagcggcgc
     4621 attgaacacc gtctgcacgt cgcagttcag gcagcgctgc gcttccttcc acgccgtggc
     4681 ggcgtcgaag ccgagttcga cttcgacccg gatgttcttc agcacctggt tgacatcgcg
     4741 ccagggtacc ttgtggcgcg catcgggcgt cacgtcgttg tcgtagctcc actcatggat
     4801 gcccatcttt tgcgacagca gcgtgacgcc ggcggcggga cgctggcgcg tgtcctcgcc
     4861 gtgcaacagc ttgtcgatcg agatcgccgc ctcgtgtccg tgtgcaacgg cccagatgat
     4921 gttcttgggt ccgagcgcgg cgtcgccgcc gaagagcacg ttgggcagcg tcgactgcag
     4981 cgtcgccttg tcgagcacgg gcatgcccga ccggtcgaac tcgatgccga gctggcgctc
     5041 gatccatgga aatgcgttct cctgccccac cgcgatcagc acttcgtcgc attcgaagat
     5101 cggatcgggc tcgccggtcg gcacgagttc gcgccgtccg ttcgcgtcgt aaaccgcctt
     5161 gaccttctcg aacgacatgc cgagcaggcg gccgtcgcga tgcaggaact ccttcggcac
     5221 cagataattg aggatcggga tgccctcgtg cagtgcgtcc tccttttccc acggcgacgc
     5281 cttcatctcc tcgaagcccg agcgcacgat caccttgaca tcctcgcccc cgagccggcg
     5341 cgcggtgcgg cagcagtcca tcgcggtgtt gccgccgcca agcacgatga cccgcttgcc
     5401 gatcttctcg acgtgcccga acgacaccga cgccagccag tcgatgccta cgtggatgtg
     5461 cgctgccgcc tcggtgcggc cgggaatcct gaggtctcgc ccgcgcggtg cgccgctgcc
     5521 gacgaacacg gcgtcgaagc cctgcgcgag caggtgctgc atcgaatcga tccgcacgcc
     5581 ggcgcggaag tccacgccga gcccgagcac gtagcccgtc tcctcgtcga tcacttcctc
     5641 gggcaggcgg aagcggggga tctgcgagcg gatcatgccg cccgatctgg cgtcctgatc
     5701 gaacaccgtg acctcgtacc cgagcggcgc cagatcgcgc gccaccgtca gcgaggccgg
     5761 gccggcgccc acgcacgcca cacgccggcc gttcctgggc gcgatcagct tgggcagccg
     5821 ggctcggatg tcgtccttga ggtcggcggc gacgcgcttc aggcggcaga tcgcgaccgg
     5881 ctcggggcgc tcggtctgcg cctgctcgac gcggccgcgg cggcaggcgg gctcgcacgg
     5941 ccgatcgcag gtgcggccga ggatgcccgg aaagacattg gacttccagt ttaccatgta
     6001 ggcgtccgag tatttgccgg ctgcaatcag ccggatgtac tcgggcaccg gggtgtgggc
     6061 ggggcaggcc ccctggcaat cgccgacctt gtgaaagtag tcaggattgc ggatttcagt
     6121 cgcttgcaac ggggtctcct tccgttggcc ggctcgctgc aggcgatccg cgttctcgca
     6181 ttgttcggac ctgcacgaag ctccctcgtt cacagtctat tactccaatg cgtgatcggc
     6241 gcgaatcgaa ccctcatgcc gttgtatgaa tgttccgcgt cagccacatg tcaggatccg
     6301 cggaaacgcc ggtgctacca tgcgcggccc tgtcggcgta tacgggaccc ggtacgctgc
     6361 aggactgcgg atcgacgcga cggaatccgt cgccgtgatc cccggacccg atgtccggac
     6421 atctttgacg agaagctcgt ttcgaaaggt gagcgccatg atgagacagt tccaggtcaa
     6481 ctcgtacctg ttcggcggca atgcgcctta cgtcgaggag ctgtacgaga aatacctcga
     6541 ccatcccgcc tccgtgccgg agaagtggcg cgcctacttc gaccagttgc agctggtgcc
     6601 ggcggccgac ggcaatccgc aatcacccga cgtcgcgcat gcaccgatcg tcgagtcgtt
     6661 cgcgctgcgc gccaagaccg gcacgctgcg gccgacggtg gcgccgaccg acctgtcggt
     6721 ggcgcgcaag caggtccacg tccagtcgat cgttgccgcg taccgcttcc tcggcgcgcg
     6781 ctgggccgac ctcgacccgc tgaagcggca ggagcggccg cacattccgg agctcgagcc
     6841 cggcttctac gacctcaccg aagcggacat ggacatcgtg ttctcggccg ccaacacgta
     6901 cttcggccag gagcagatga cgctgcgcga gatcgtcaag gcgctgcgcc agacgtactg
     6961 cagcacgatc ggcgtcgagt acatgtacat ctcggacccg gccgagaagc gctggtggca
     7021 gcaacggctc gagtccattc gctcgacgcc gacctacacg gctgagcaga agacgcacat
     7081 cctcgagcgg ctgaccgcgg cggaggggct ggagcgctac ctgcacacca agttcgtcgg
     7141 ccagaagcgt ttctcgctcg aaggcggcga gagcttcatc cccgcgatgg acgagatcgt
     7201 gatgcacgcc ggcgagatgg gcgtggagga aatcgtgatc ggcatggcgc accgcggacg
     7261 cctgaacgtg ctggtcaaca cgctcggcaa gatgccgaag gacctgttcg cggagttcga
     7321 gggcaaggcc gcctccgacc tgcccgccgg cgacgtcaag tatcacaacg gtttctccag
     7381 cgacgtgacc acgccgggcg gcccggtgca tctgtcgctc gcgttcaacc cctcgcatct
     7441 ggagatcgtc aacccggtgg tggaaggctc cgtgcgcgcg cggcaggagc gcaggggcga
     7501 caacaagcgc gagaaggtgc tgccggtgct ggtgcacggt gacgccgcct tcgccggcca
     7561 gggcgtggtt atggagacgc tgaacctggc gcagacgcgc ggctacaaga ccggtggcac
     7621 cgttcacatc atcatcaaca accagatcgg cttcaccacg tccgatccgc gcgattcgcg
     7681 ctcgacgttg tactgctcgg acgtcgtcaa gatgatcgag gcgcccgtcc tgcacgtgaa
     7741 tggcgacgat cccgaggcgg tcgtgctgtg cgcgcgcttc gcgatggagt accggcagca
     7801 gttcaagaag gatgtggtga tcgacatcat ctgtttccgc cgcctggggc acaacgagca
     7861 ggacacgccg gcggtcacgc agccgttgat gtacaagaag atcgggagcc acgcgggaac
     7921 acgcaggctg tacgccgacc gactggtcac gcagggcacg ttgcgcggcg aaacgccgga
     7981 tgagatggtc aaggcatacc gggcggcgat ggacgcgggc cgccacacgg tcgacctcgt
     8041 tctgaccaac tacaagagca agtacgcggt cgactggtcg ccgttcctga accgcaggtg
     8101 gaccgatcac gccgataccg cggtgccgat cgccgagctg aagcggctcg ccgagcgcat
     8161 caccacggta cctgccaact tcaagctgca tccgctggta gacaaggtga tcgccgatcg
     8221 gcgcgcaatg ggcgaaggcc gggtcccgct cgactggggc atggcggagc atctgtcgtt
     8281 cgcgtcgctg gtcgccagcg gttatccggt gcgcatcacc gggcaggatt gtggccgcgg
     8341 cacgttctcg caccgccacg cggtgttgca cgaccagaat cgcgagcggt gggacgccgg
     8401 cgtctacgtg ccgctgcaga acgccgccga gaaccaggcg ccgtttttgg tgatcgactc
     8461 ggtgctgtcg gaggaggcgg tgctcggctt cgagtacggt tattcgtgcg ccgagcccaa
     8521 tgcgctggtg atctgggaag cgcagttcgg cgatttcgtc aatggcgcgc aggtggtgat
     8581 cgaccagttc atcagctcgg gcgaagtgaa gtggggtcgg cagtcgggac tgacgctgat
     8641 gctgccgcac ggctacgaag gtcaggggcc cgagcactcg tcggcgcgga tcgagc
//
LOCUS       DBNHBY010000372         3408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_630648, whole genome shotgun sequence.
ACCESSION   DBNHBY010000372 DBNHBY010000000
VERSION     DBNHBY010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_630648"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC16HB_09220"
     CDS             <1..306
                     /locus_tag="AC16HB_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607008.1"
                     /translation="TALVISVSAVLGFAAIGVRADLLAFHRIDVAADRVTLHYALPAR
                     TRTVPRADIARVALGLGSEKGPTVRLVLYTRSGERHESAPTPRARFDAARVALAVAE"
     gene            316..2100
                     /locus_tag="AC16HB_09225"
     CDS             316..2100
                     /locus_tag="AC16HB_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR5607009.1"
                     /translation="MTIRYAIRPAHPGAHLFHVTVSLDDPDPAGQRLMLPAWIPGSYM
                     LREFARHIVRLTALSGDRKVRVDKLDKHTWRCAHVPGPLTVAYEVYAWDLSVRAAHLD
                     ETHAFFNGACVFLLPLGRAHEACEVDVLPPAGRRFADWKLVTGLTPARGTKRGAFGTY
                     AAQDYDELIDCPVEMGTFAHARFDVLGTPHEIAITGRVPKLDLKRLTVDLTRLCETQV
                     RLFEPRTKRAPFDRYAFLTFALGEGHGGLEHRNSTALICKRDGLPFTGMRDSTESYRN
                     FLGLASHEYFHSWNVKRIRPAAFVPYDLTRENYTRLLWAFEGFTSYYDDLVLARSGLL
                     TEAQYLDALAQTMTTVMQRSGRLKQSLGDSSFDAWIKYYRPDENAPNSMVSYYQKGAL
                     VALALDLTIRVATRGRRSLDDVMRLLWKRWKDVPKYPGLDEDEIVEAAETATSLKLAR
                     AIREWTEGTRDPDFAALLAPFGVACERRSALDAPHFALLGIKTAARGESSVANVFDGG
                     TAQDAGVSGGDVLVALNGLRIHGGNLDALLARYEPGDRVELHAFRRDELMRFEVKLGT
                     QPPPKFVLRADPRASKAAARLRLGWLRG"
     gene            2105..>3408
                     /locus_tag="AC16HB_09230"
     CDS             2105..>3408
                     /locus_tag="AC16HB_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5607010.1"
                     /translation="MAEAVALPTHARIVVIGGGIIGCSVAYHLAHLGVTDVVLLERDR
                     LTSGTTWHAAGLIVTFGSTSETSTELRKYSRSLYARLEAETGQATGFKPCGFIEVATD
                     ADRLEEYRRVAAFNRYCGIDVHELLPREVRALFPLAKFDDMLAGFYVKEDGRANPVDV
                     TMALARGAKLKGARIAEGVAATRVLAQRSAVTGVETTHGTIRCETVVNCAGMWARELG
                     ALDGVNIPLQPAEHYYLITGPLPGIGRDWPVLEDPASYGYYREEGGGLMIGLFEPVCA
                     PWKVEGVPQDFSFGEIEPDWDRMTPYLERAMARVPASQAAGIRKLFCGPESFTPDLQP
                     IIGEAPALANYFVAAGLNSIGILTGGGIGRLLAHWIVTGAPDADVTGIHIDRLHSYQS
                     NPEYRRTRTVESLGMVYQCHYPTRSMQTARGAKRSPLHDRLAA"
BASE COUNT          541 a         1177 c         1175 g          515 t
ORIGIN      
        1 accgcgctcg taatctcggt gagcgcagtg ctcggtttcg cggcgatcgg cgtccgcgcc
       61 gatctgctcg cgttccatcg catcgatgtc gccgccgacc gcgtcacgct gcactacgcg
      121 ctgcccgcgc gcacgcgcac agtgccgcgc gcggatatcg cacgagttgc actcggactc
      181 ggcagcgaga aaggcccgac cgtgcgcctc gtgctgtaca cgcgcagcgg cgagcgccac
      241 gagagcgcgc caacgccgcg agcccgcttc gacgcggcgc gcgtcgcact cgcagtcgct
      301 gagtaaactc gcccgatgac gatacgctac gcgatccgcc cggcgcaccc gggcgcgcac
      361 ctgtttcacg tcaccgtctc cctcgacgat cccgatccgg ccggccagcg gctgatgctg
      421 ccggcctgga ttcccggcag ttacatgctg cgcgagttcg cgcgccacat cgtgcggctc
      481 accgccctct caggtgaccg caaggtgcgc gtcgacaaac tcgacaagca cacctggagg
      541 tgtgctcacg tccccggccc gctgaccgtc gcatacgagg tgtacgcgtg ggacctgtcg
      601 gtgcgcgccg cccatctgga cgaaacgcac gccttcttca acggcgcctg cgtgttcctg
      661 ctaccgctcg ggcgcgcgca cgaggcgtgc gaggtcgacg tgctgccgcc cgccggccgc
      721 aggttcgccg actggaaact ggtgacggga ctgacgcccg cgcgcggcac gaagcgcggc
      781 gccttcggca cctatgccgc gcaggattac gacgagctga tcgactgccc ggtcgagatg
      841 ggcacgttcg cgcacgcgcg gttcgacgtg cttggcacgc cgcacgaaat cgcgatcacc
      901 ggtcgcgtgc cgaagctgga cctgaagcgc ctgaccgtcg acctcacccg tctgtgcgaa
      961 acccaggttc gcctgttcga gccgcgcacg aagcgggcgc cgttcgaccg ctacgcgttc
     1021 ttgacctttg ccttgggcga gggccacggc gggctcgagc accgcaactc caccgcgctg
     1081 atctgcaagc gcgatggtct gccgttcact ggcatgaggg actcgaccga gagctaccgc
     1141 aacttcctcg ggctggcgag ccacgagtac tttcacagct ggaacgtcaa gcgcatccgg
     1201 cccgcggcgt tcgtgccgta cgacctgacc cgcgagaact acacgcgcct gctgtgggcg
     1261 ttcgagggct tcacctcgta ctacgacgac ctggtgctgg cgcggtccgg gctgctgacc
     1321 gaggcgcagt acctcgatgc gctggcgcaa acgatgacga cggtgatgca gcgctcggga
     1381 aggctgaagc agagcctcgg cgacagctcc ttcgacgcct ggatcaagta ttaccgtccg
     1441 gacgagaacg cgccaaacag catggtcagc tactaccaga agggcgcgct cgtggcactt
     1501 gcactcgatc tgaccatccg tgtggccacg cgcggacgca ggtcgctcga cgacgtaatg
     1561 cgactgctgt ggaagcgctg gaaggacgtg cccaaatatc ccggcctcga cgaggacgag
     1621 atcgtcgaag cggcagaaac cgcgacgagt ttgaagctcg cgcgcgcgat ccgcgagtgg
     1681 accgagggca cgcgcgaccc cgatttcgcg gcgctgctgg cgccgttcgg tgtggcgtgc
     1741 gagcggcgct ccgccctcga cgcgccgcac tttgcgctgc tcggcatcaa gaccgccgcc
     1801 cgcggcgagt ccagcgtggc caacgtgttc gacggcggca ccgcgcagga cgcgggcgtc
     1861 tccggcggcg acgtgctggt ggcgctgaac ggcctgcgca tccacggcgg caatctcgat
     1921 gcgctgctgg cgcgctatga gccgggcgac agggtcgagc tgcacgcgtt ccgccgcgac
     1981 gagctgatgc gcttcgaggt gaagctcggc acgcagccgc cgccgaagtt cgtgctgcgc
     2041 gccgatccga gggcgagcaa ggcggccgcg cgcttgcgac tcggctggct gcgaggctga
     2101 ggcaatggcc gaagcggtgg cgctgccgac ccacgcacgc atcgtcgtga tcggcggcgg
     2161 catcatcggg tgctcggtcg cgtatcacct cgcgcacctg ggcgtgaccg acgtggtgct
     2221 gctcgaacgc gaccggctga cctcgggcac gacgtggcac gcggccgggc tgatcgtcac
     2281 gttcggctcg acctcggaga cctcgaccga gctgcgcaag tactcgcgga gcctgtacgc
     2341 gcggctcgag gcggagaccg gtcaggcgac gggattcaag ccgtgcggct tcatcgaagt
     2401 cgcgaccgac gccgaccgcc tcgaggagta tcgccgcgtc gccgcgttca accgctactg
     2461 cggcatcgac gtgcacgaat tgctgccgcg cgaggtgcgc gcgctgttcc cgctggcgaa
     2521 gttcgacgac atgctggccg gcttctacgt gaaggaagac ggccgcgcca atccggtcga
     2581 cgtgacgatg gcgctcgcca ggggcgcgaa actgaagggc gcgcggatcg ccgaaggcgt
     2641 ggcggccacc cgcgtgctcg cgcagcgcag cgcggtgacc ggtgtcgaaa cgacgcacgg
     2701 cacgatccgc tgcgagacgg tcgtcaactg cgccggcatg tgggcgcgcg aactgggcgc
     2761 actcgacggc gtgaacatcc cgctgcagcc agccgagcac tactacctga tcaccgggcc
     2821 gctgccgggc atcggacgcg actggccggt gctcgaagac ccggcctcgt acggctatta
     2881 ccgcgaggaa ggcggcggcc tgatgatcgg cttgttcgag cccgtgtgcg cgccgtggaa
     2941 ggtcgagggc gtgccgcagg acttctcgtt cggcgagatc gagcccgatt gggaccggat
     3001 gacgccgtac ctcgagaggg ccatggcgcg cgtgccggcg tcgcaggcgg cgggaataag
     3061 gaaactcttc tgcggtccgg agagcttcac gccggacttg cagccgatca tcggcgaagc
     3121 gccggcgctc gcgaactatt tcgtcgccgc cgggctgaat tcgatcggca tcctgaccgg
     3181 tggcgggatc gggcgactgc tcgcgcactg gatcgtgacc ggcgcgcccg acgcggacgt
     3241 gaccggcatc cacatcgacc ggctgcattc gtatcagtcg aaccccgagt accggcgcac
     3301 gcgcacggtc gagtcgctcg gcatggtgta ccagtgccac tatccgacgc gctcgatgca
     3361 gaccgcgcgg ggcgccaagc gctcgccgct gcacgaccgg ctcgcggc
//
LOCUS       DBNHBY010000373         2426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_562953, whole genome shotgun sequence.
ACCESSION   DBNHBY010000373 DBNHBY010000000
VERSION     DBNHBY010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_562953"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC16HB_09235"
     CDS             complement(<1..319)
                     /locus_tag="AC16HB_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HIR5607011.1"
                     /translation="MDARPSFEQTVPGDAARATLIGRAWMPGAGGGPTPVAVRDGALV
                     DISCVAPTVSGLLELSDPVAAVRAAPDTRIGRVAEALGNPPRADRLHLLAPCDLQPIK
                     AAGV"
     gene            514..1179
                     /locus_tag="AC16HB_09240"
     CDS             514..1179
                     /locus_tag="AC16HB_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607012.1"
                     /translation="MLSAAGLLCLTSCATIQSQSGADNRMSFFVTSVGPGKGGDLGGL
                     EGADRHCQSLAAAVGAGDRTWHAYLSTQGAALNDPKVVHARNRIGTGPWYNAKGVLIA
                     RSVDDLHSANNNVNKETALDERGMRVNSRGDTPLKHDILTGSRPDGTAFPGPPFSDMT
                     CGNWTRSGKEGAAMTGHHDSAGPINHPWATSWNSSHPTLGCSQEALRPTGGDGLFYCF
                     AVK"
     gene            1278..2258
                     /locus_tag="AC16HB_09245"
     CDS             1278..2258
                     /locus_tag="AC16HB_09245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5607013.1"
                     /translation="MQYVNLGRTGLKVSRICLGCMTFGVPSRGTHRWTLEEDATRPII
                     RHAIERGINFFDTANSYSDGTSEEFVGRALKDFARRDEIVLATKVFNKWRDAPNTGGL
                     SRKAIFTAIDDSLRRLGVDYVDLYQTHRWDYTTPIEETMEALHDVVKAGKARYIGASS
                     MYAWQFAKALHVAERRGWTRFVTMQPHLNLLYREEEREMLPLCADAGIGVIPWSPLAR
                     GRLTRDWDVKTARTETDEYGKSLYTANDAVYRAIVDAVAATARQRGVSRAQVALAWVM
                     GNPGITAPIIGASKPQHIDDAIAALDVKLSEEERARMEAPYVPQPVVGFK"
BASE COUNT          413 a          844 c          832 g          337 t
ORIGIN      
        1 tcacgccggc tgccttgatc ggctgcaggt cgcacggggc gagcaggtgc aggcgatccg
       61 cgcgcggtgg attgcccaat gcctccgcca cgcgaccgat ccgcgtgtca ggcgccgcgc
      121 gcaccgccgc gaccggatcg gacaattcca gcaggccgct gacggtcggg gcaacgcagg
      181 agatgtcgac gagtgcgccg tcacgcacgg cgaccggcgt gggtccgccg ccggcaccgg
      241 gcatccacgc gcggccgatc agggtcgcgc gcgccgcgtc gccgggaacg gtctgctcga
      301 atgaaggtcg ggcatccatc cctgccagtg tgccacggcc aacgcgcggc cagcggcgcg
      361 cacggcatgc accgcgcgac gctcgatgcc ccgacgaggt gatcccttcc cgtacgcgcg
      421 attgcgtacc gactcggcca ccgcgataag ctgcggctcg cgaggttgaa cccactggag
      481 gaagttcgta tgcgaggcct gatgcgttgt tcgatgctgt ccgccgccgg gttgttgtgc
      541 ctgaccagtt gcgccacgat ccagtcgcag tcgggcgcgg acaatcgaat gtcgttcttc
      601 gtcaccagcg tcgggccggg gaagggcggc gacctcggcg gcctcgaagg cgcggaccgg
      661 cactgccagt cgctcgccgc cgccgtcggc gcgggcgacc gcacctggca cgcctatctg
      721 agcacgcagg gcgccgcact gaacgatccc aaggtggtcc atgcacgcaa ccgcatcggc
      781 accggcccct ggtacaacgc caagggagtg ctgatcgcca ggagcgtcga cgacctgcat
      841 tcagccaaca acaatgtgaa caaggagacg gcgctggacg aacggggcat gcgggtcaac
      901 agccgcggag acaccccgct caagcacgac atcctgaccg gctcgcggcc cgacggcacc
      961 gcgttcccgg gcccgccgtt ctccgacatg acgtgcggca actggacccg cagcggcaag
     1021 gaaggtgcgg caatgaccgg gcaccacgac agcgccggcc cgatcaacca tccgtgggcg
     1081 acctcgtgga actcgtcgca tccgacgctg gggtgcagcc aggaggcatt gcgcccgacc
     1141 ggcggtgacg gcctgttcta ctgttttgcc gtgaaatagc gcaacggacg cgaccggcgg
     1201 gcgtcgcccc accgggacga tgcccgctcc tgtttgcgca cggcgacttc caccggcagg
     1261 aacccgagta cacccgcatg cagtacgtga acctcggccg caccggcctg aaagtgtccc
     1321 gcatctgcct gggctgcatg acgttcggcg tgccgagccg cggcacccac cggtggacgc
     1381 tggaagagga cgccacgcgg ccgatcattc ggcacgcgat cgaacgcggc atcaacttct
     1441 tcgacaccgc caactcgtac tcggacggca cgtcggaaga gttcgtcggg cgcgcgctga
     1501 aggacttcgc gcgacgcgac gagatcgtgc ttgccaccaa ggtcttcaac aagtggcgcg
     1561 acgcgcccaa caccggcggc ctgtcgcgca aggcgatctt cactgcgatc gacgacagcc
     1621 tgcggcggct cggcgtcgac tacgtcgacc tgtaccagac gcaccgctgg gactacacga
     1681 cgccgatcga agaaacgatg gaagcgctgc acgatgtcgt caaggccggc aaggcgcgct
     1741 acatcggcgc gtcttcgatg tacgcgtggc agttcgccaa ggcgctgcac gtggcggagc
     1801 gccgcggctg gacgcgcttc gtgaccatgc agccgcacct gaacctgctg tatcgcgagg
     1861 aagaacggga gatgctgccg ctgtgcgccg acgccggcat cggcgtgatt ccgtggagcc
     1921 ctctcgcgcg cggccggctc acccgcgact gggacgtgaa gaccgcgcgc accgagacgg
     1981 acgagtatgg caagtcgctc tacaccgcca acgacgcggt gtatcgcgcg atcgtcgatg
     2041 cggtcgccgc gactgcccgg cagcgcggcg tgtcgcgcgc gcaggtggcg ctggcgtggg
     2101 tgatgggcaa ccccggcatc acggcgccga tcatcggcgc atcgaagcca cagcacatcg
     2161 acgatgcgat cgccgcgctc gacgtgaagc tcagcgaaga agagcgcgcc cggatggagg
     2221 cgccctacgt gccgcagccg gtcgtcggct tcaagtaagg cagagcgggc ggccgccggc
     2281 gaaccgtccg tcgctacggc tcgatgatca cctcggtccg acgcgctttt tccggcgcgc
     2341 cgctgccggt cgttcgggct ggcctggcga cgatgatgcg gtcgcgcgca acgccggcgt
     2401 gctcgagcgc ggcgcgcact gcaatc
//
LOCUS       DBNHBY010000374         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1714150, whole genome shotgun sequence.
ACCESSION   DBNHBY010000374 DBNHBY010000000
VERSION     DBNHBY010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1714150"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2065)
                     /locus_tag="AC16HB_09250"
     CDS             complement(<1..>2065)
                     /locus_tag="AC16HB_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5607014.1"
                     /translation="LRQLRGILVPLDHLLAGARSIADRNFSARVKVNARDEFGRVASA
                     FNAMAERLGVHFRALHTFSEIDKTILTSPDTGKIAKLAIDTLKEITHADYVSLGVSEP
                     GSPSQLRIYICDGPDGELAPAREIAWNPDPRSFADRATCRTWTETPPLPNGYLQAIKP
                     GDGMKLFVQPIARNRRIWGVIVLGQREPRELASHRRALIRDIADRLAVALSTAERDRR
                     MHVLAHLDGLTGLPNRQRLLTILEQELTAAGEGKQRVGVLFIDLDRFKETNDTLGHAA
                     GDTLLKRTSERIRENVRETDLVGRHGGDEFVVVLRNVGAPADAGTVARKLVKELGKPF
                     EVEGHNVYAGASIGIAFFPDDGRDAADLLKKADTAMYQAKEEGRGRYAFFKQSMNVEM
                     NRRVTLDRELRQALERNEFTLFFQPQIDLRTGRICSAEALLRWRHPKRGLLRPSHFID
                     LAEETGLIEKLGAWAMREACEQHGRWRAQRVNIPRVAVNVSSRQLRLPEFVPTVDYTM
                     VATQMAPDSLEVEVTESLILDGGEVAISALRALERAGARVAIDDFGTGYSSFTYLKTL
                     PASILKLDRSFVADIATDKDAATIAAAIINMAHTLRKEVVAEGVETAEQLAFLRAAGC
                     EKVQGYLLSPPLPADRLAALARCAETGGVAAVIEETRRRMSGATVLESAPITAAPPDL
                     SNLVTV"
BASE COUNT          306 a          692 c          711 g          356 t
ORIGIN      
        1 gcacggttac gaggttggac aggtcgggcg gtgccgcagt gatgggcgcg gattcgagca
       61 cggtggcgcc gctcatgcgc cggcgcgttt cctcgatcac ggccgccacg ccgccggttt
      121 ccgcgcaacg tgccagcgcc gccagccgat ccgccggcag cggcgggctg agcaggtagc
      181 cttgcacctt ctcgcagccg gctgcacgca gaaaggcgag ctgctcggcc gtctcgacac
      241 cctcggccac gacctccttg cgcagcgtgt gcgccatgtt gatgatcgcc gccgcgatcg
      301 tcgccgcgtc cttgtcggtc gcgatgtcgg cgacgaacga cctgtcgagc ttcagaatcg
      361 acgccggcag ggtcttcaga tacgtgaacg acgaatagcc ggtgccgaag tcatcgatgg
      421 ccacgcgcgc accggcacgc tcgagcgcgc gcaaggccga gatcgcgacc tcgccgccgt
      481 cgagaatcag cgactccgtg acctcgacct ccaggctgtc gggcgccatc tgcgtggcga
      541 ccatcgtgta gtcgacggtt ggcacgaatt ccggcaggcg cagttgccgg ctcgacacgt
      601 tgaccgccac gcgcggaatg ttcacgcgct gggcgcgcca gcggccatgc tgttcgcagg
      661 cctcgcgcat cgcccacgcg ccgagcttct cgatcaaccc ggtctcctcg gccagatcga
      721 tgaagtggct cggccgcagc agcccgcgct tcgggtgccg ccagcgcagc agcgcttcgg
      781 cgctgcagat acgcccggtg cgcaggtcga tctgcggctg gaagaacagc gtgaactcgt
      841 tgcgctccaa tgcctgccgc agctcgcgat cgagcgtcac gcgccggttc atctcgacgt
      901 tcatgctctg cttgaagaac gcgtagcggc cacggccctc ttccttggcc tggtacatcg
      961 cggtatcggc cttcttcagc agatcggcgg cgtcgcggcc gtcgtcgggg aagaacgcga
     1021 tgccgatgct ggcgccggca tacacgttgt gcccttcgac ctcgaacggc ttgccgagct
     1081 ccttcaccag tttgcgcgcg actgtgccgg cgtcggcggg tgcaccgaca ttgcgcagca
     1141 ccacgacgaa ttcgtcaccg ccgtggcggc cgaccagatc cgtttcgcgc acgttctcgc
     1201 ggatgcgctc ggaggtgcgc ttcagcagcg tgtctccggc cgcgtgcccc agcgtgtcgt
     1261 tggtttcctt gaaccggtcc agatcgatga acagcacgcc gacgcgctgc ttgccctcgc
     1321 cggcagcggt cagctcctgc tccaggatcg tcagcaggcg ctggcggttg ggcaacccgg
     1381 tgaggccgtc cagatgcgcc agcacgtgca tgcgccggtc gcgctcagcg gttgacagtg
     1441 cgacggcgag gcggtcggcg atgtcgcgga tcagcgcgcg gcgatggctg gcgagttccc
     1501 gcggttcacg ttggccgagc acgatcacgc cccagatgcg ccggttgcgc gcgatcggct
     1561 gcacgaacag cttcatgccg tcgccgggct tgattgcctg caggtagccg ttcggcagcg
     1621 gcggcgtctc ggtccaagtg cgacaggtgg cgcgatcggc gaagctgcgc ggatccggat
     1681 tccatgcaat ctcgcgcgcg ggagccagct cgccgtccgg tccatcgcag atatagatgc
     1741 gcagctgcga cgggctgccg ggttcggaca cgccgaggct gacgtagtcg gcgtgcgtga
     1801 tctccttcag cgtgtcgatc gccagtttgg cgatcttgcc ggtgtcaggg gaagtcagga
     1861 tcgtcttgtc gatttcggag aatgtgtgca gcgcgcggaa gtgcacgccg agccgctcgg
     1921 ccatggcgtt gaacgcggag gcgacacggc cgaattcgtc gcgcgcattg accttcacgc
     1981 gcgcggagaa gttgcggtcg gcgatgctgc gcgcgccggc gagcaggtga tccagcggca
     2041 ccaggatgcc gcgcagctgg cgcag
//
LOCUS       DBNHBY010000375        21346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_361025, whole genome shotgun sequence.
ACCESSION   DBNHBY010000375 DBNHBY010000000
VERSION     DBNHBY010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 21346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21346
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_361025"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..588)
                     /locus_tag="AC16HB_09255"
     CDS             complement(67..588)
                     /locus_tag="AC16HB_09255"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607015.1"
                     /translation="MAPELRSVRAGDLPAIRDIYAYHVTHGLASFELDAPSLEEMRSR
                     HVAITRGGYPYLVAHDGGRVVGYAYANHYRTRPAYRYSVEDSIYIAPDATGRGIGRQL
                     LVRLIAECEARGFRQMLAVIGDSANAPSIELHRACGFAQVGLLPSIGFKFGRWVDSVL
                     MQRPLGDGDRKLP"
     gene            complement(669..2846)
                     /locus_tag="AC16HB_09260"
     CDS             complement(669..2846)
                     /locus_tag="AC16HB_09260"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR5607016.1"
                     /translation="MSGLALALLCGLAAVVFGALSISWILKQPAGNPRMQEIAAAIQT
                     GAQAYLNRQYRTIAIVGVVLAIVIAVVPQLGLWTAIGFVLGAVLSGAAGFIGMNVSVR
                     SNVRTAQAATQVLNAALNIAFRGGAITGLLVVGLGLLGVAGFYWLLKTTHAAANTREL
                     LHPMIGFAFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETFAVTVVATMLIGGFFFKSGGDAGIIYPLAISGVSILASIVG
                     CFFVKARDGGKIMNALYRGLIVAGVISLVLFWPVTNWILGPVAASGGVTVMNLYLSAV
                     VGLVLTAALVYITEYYTGTQFTPVKYIAQASTTGHATNIIAGLAVSMKACAWPVLAIS
                     AAIYASYGLAGLYGIAVAATSMLSMTGIIVALDAYGPITDNAGGIAEMAGLPKEVRNI
                     TDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTEGLKSFGLSTNFDLSDHKVIIGL
                     FIGGLIPFLFGAMAMEAVGRAAGSVVEEVRRQFREIKGIMEGTAKPDYSRAVDMLTTA
                     AIKEMMIPSILPVLVPIIVGIVLGPAALGGVLMGTIVTGLFVAISMTTGGGAWDNAKK
                     YIEEGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPIIASIH
                     GGKPVAAMPVTPPAVVAPATAPTTAPAPAPAPAPEAKKEEEKK"
     gene            complement(2880..3536)
                     /gene="adk"
                     /locus_tag="AC16HB_09265"
     CDS             complement(2880..3536)
                     /gene="adk"
                     /locus_tag="AC16HB_09265"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5607017.1"
                     /translation="MRVILLGPPGAGKGTQAAFITQRYGIPQISTGDMLRGAVKAGTP
                     TGVAAKRVMDAGQLVSDDIIVALVKERLKQPDCAAGYLFDGFPRTIPQAEALRAAGIK
                     VDVVLEIDVPDADIIERMSGRRVHLASGRTYHVKFNPPKVPDRDDTTGEPLIQRDDDR
                     EEVVRKRLQVYHAQTRVLVDYYAKWSAGGDTAAPRHVRVSGVGTVGEIKQRVLDALAQ
                     "
     gene            complement(3698..4960)
                     /locus_tag="AC16HB_09270"
     CDS             complement(3698..4960)
                     /locus_tag="AC16HB_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5607018.1"
                     /translation="MKKRNAFYAQSGGVTAVINASAAGVIETARRYKSRIGKVYAGRN
                     GIIGALTEDLIDTSRESAATIKALKHTPAGAFGSCRYKLKGLEESRAQYERLIEVFRA
                     HDIGYFFYNGGGDSADTCLKVSQLSDKLGYPLQAIHVPKTVDNDLPITDVCPGFGSVA
                     KYIAVSTREASFDVASMAKTSTKVFVLEVMGRHAGWIAAAGGLASDRTHPIPIVILFP
                     EVPFDRDGFLAAVKDKVERLGYCSVVVSEGVKGTDGKFLSDQGLRDAFGHAQLGGVAP
                     VIASLVKESLNYKFHWAVADYLQRAARHIASKTDVEQAYAVGKAAVEFALKGHNAVMP
                     AIVRTSSRPYRWKIEMAPLADVANREKTMPREFISADGFGITEACRRYLSPLIRGEAY
                     PPYKDGLPDYAVLKNVAVKKRLPNVFKI"
     gene            complement(5081..6649)
                     /locus_tag="AC16HB_09275"
     CDS             complement(5081..6649)
                     /locus_tag="AC16HB_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607019.1"
                     /translation="MNRWYAALAALLLVVSGAGAKTLRWASQGDFQTADPYSQNELLT
                     NLLSQQVHDTLVTRDKELRIVPGLAVSWSQPSPLTWRFNLRKGVKFHDGTPFTADDVV
                     FSFERASHANSQIRQYAIPVTGAPRKIDDYTVEFVQDKPNPVMLQHVATIFIMSKAWS
                     EKHKVERPLSFKDREETFAARNANGTGPYMLAVREPGVKTALKRNPNWWGTFEGNVDE
                     IVYTPIASDATRVAALLSGQVDFILDPPPQDLDRLRATAGVKVVDGMENRVVFFGFDQ
                     ARDELLYSNVKGRNPFKDARVRRAVYQAIDIEALRSRTMRGAALPTGGITPSILASNP
                     EAEKRLPYDLSEAKRLLAEAGYPNGFEVTLDCPNNRYINDEKICLAVAAMLSKVGIQT
                     RVTAQPRATYFPKLEKLDTSFYMLGWGGAITDAQTTLSPVLRSFDPKSGNGSFNYGRY
                     ANPKLDALIDAAAIEMNAEKRAEIIRQALAEHNAQVHHVPLHRQVIPWAMRANVHVTH
                     RADNWLMGEWTRID"
     gene            complement(6684..7451)
                     /gene="kdsB"
                     /locus_tag="AC16HB_09280"
     CDS             complement(6684..7451)
                     /gene="kdsB"
                     /locus_tag="AC16HB_09280"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR5607020.1"
                     /translation="MAGFVVIVPARLASTRLPRKMLADVGGLPLIVRVAQRSAESHAQ
                     RVIVAADSEEIARACAAQGVSAVLTSTRHPTGTDRVAEAAQQLALPDDAIVVNVQGDE
                     PLIPPAVIDAVAARLQAQPDCAMATVAHPVAEAAEFFSPNVVKVVTDARGRALLFSRA
                     PIPWSRDAFAQTRGRLPDGLPALRHVGLYAYRAGFLRRYPHLPPAPIEAHESLEQLRA
                     LWHGHAIAVLRLDAPLPPGVDTFEDLEHVRALIAGRA"
     gene            complement(7501..7665)
                     /locus_tag="AC16HB_09285"
                     /pseudo
     CDS             complement(7501..7665)
                     /locus_tag="AC16HB_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
     gene            complement(7646..8740)
                     /gene="lpxK"
                     /locus_tag="AC16HB_09290"
     CDS             complement(7646..8740)
                     /gene="lpxK"
                     /locus_tag="AC16HB_09290"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR5607021.1"
                     /translation="MNRPDLTARLERTLHRVWRTRGPAAWLLLPLALVYGAVRALIAW
                     SYRIGVRRAWHVGVPVIVVGNLYVGGTGKTPLTLELVRALLLRGRRPGVVTRGYGAGG
                     NDVRTVETHARATEVGDEPLLLAQALQGQGVPVVVGRDRVAACRILRSVHPRCDVIVA
                     DDGLQHHRLARDFEIALLDERGLGNGWLLPAGPLRDPPARLKEVAAVVCHGIVPPVRV
                     YSPRYEMVSRLGDVRSLADPTRMISLEGLVQEQRRGQLKIIAAAGIGAPERFFGMLRA
                     AGLDIEELPLPDHYDYAVNPFLGRKFDLALITAKDAVKCRAVSAIATDGRVCVVGLQA
                     TIDAGLIDLIEMRLASPAVQHEAAHGSTTA"
     gene            complement(8742..9167)
                     /locus_tag="AC16HB_09295"
     CDS             complement(8742..9167)
                     /locus_tag="AC16HB_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5607022.1"
                     /translation="MKFRRARDEEPELNLIPLIDVMLIVLIFLAVTTTYSRFAELKIE
                     LPRAEAKATQNRPNEVVIGVTADGRYAIDKRVLAKSDVDTLVEALRAAAQGRTDLLVV
                     IHADGQSPHQSVINVMEAARIAQLSRISFVTERQNTAAQ"
     gene            complement(9164..9781)
                     /locus_tag="AC16HB_09300"
     CDS             complement(9164..9781)
                     /locus_tag="AC16HB_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5607023.1"
                     /translation="MFAIVQAIGWPIWPLLAASVVALTLIIERSIALRRGKVIPESLL
                     QDVLNLVERRQVNSEVILKLERNSPLGRVLATALRNVRAPRAIAGAAVANTGGHIAHD
                     LSRYLSLLGSIGSVAPLLGLLGTVIGMIDIFASQQAGSNPQQLAHGISIALYCTAFGI
                     IVAVPSVLFYRHFRSRIEDFLVEMEDQSQRLLEVLFADRYDATPR"
     gene            10089..11471
                     /gene="xseA"
                     /locus_tag="AC16HB_09305"
     CDS             10089..11471
                     /gene="xseA"
                     /locus_tag="AC16HB_09305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5607024.1"
                     /translation="MTPEFADANLATTRDPLTVSALNRVVAGLLARAFPLVRVRGEIA
                     NFTRAASGHWYFTLKDPGAQVRCAMFRGRNGLVDFAPREGDHVDVLAQVGLYEARGEF
                     QLNVESMQRAGLGRLYEEFLRLKARLAAEGVFDEAQKRRLPLLPRAVGIVTSLQAAAL
                     RDVLTTLRRRAPYLRLIVYPVPVQGEGAGARIAQMLQRVSARAEVDAVLLVRGGGSIE
                     DLWSFNEEPVARAIRASALPVVVGVGHESDFTIADFAADLRAPTPTAAAELVAPDRSG
                     LVDSVTQKLRSLRVALRRRNEIAQQRFDYALRSLAVPRAPLRALEARVASAVTQLRHL
                     VARTAVARRAATARCLQRLSRRRPDALLRRARLLQACARLCASAQRQHKSRQGRFEAL
                     AAKLGALDPHAVLVRGYAMAVAPDGRVVRDARQLAPGDRLRLQFARGGAHARVEDLEL
                     RAGNTDAPDD"
     gene            11532..12113
                     /gene="sodB"
                     /locus_tag="AC16HB_09310"
     CDS             11532..12113
                     /gene="sodB"
                     /locus_tag="AC16HB_09310"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HIR5607025.1"
                     /translation="MEHTLPPLPYAMDALAPHISKETFEYHYGKHHQAYVTNLNNLIK
                     GTEFEASSLEDIVKKSSGGIFNNAAQVWNHTFFWNSMKPGGGGEPAGALGAAIARKWA
                     SYAAFKEAFAKSAVGNFGSGWTWLVKKADGSVEIVNTSNAATPLTTADRALLTIDVWE
                     HAYYIDYRNARPKFVETFLNNLANWGFAAKNFA"
     gene            complement(12158..12676)
                     /locus_tag="AC16HB_09315"
     CDS             complement(12158..12676)
                     /locus_tag="AC16HB_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR5607026.1"
                     /translation="MSVTDEGAGLQGPALFLLKARDAMKKLVRALLGVCLALVSILAG
                     AQGPLPPQPAIKLNVGIHLITAEVVADPVTTTRGLMFRERLEPNHGMLFVYRAKSPMC
                     FWMRNTLVPLTIAFIDDDGTIVDLEDMAPRTENQHCSSKPVRFALEMERGWFDKRGLK
                     VGAKINGLPRPR"
     gene            complement(12679..12921)
                     /locus_tag="AC16HB_09320"
     CDS             complement(12679..12921)
                     /locus_tag="AC16HB_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607027.1"
                     /translation="MKKAIAALISALFAGAVFAQAAPAAPAKPATPATPATPVAGPSA
                     APAAPKAEAKAEAKEVKKAKAVKKARKAKKAEAEKK"
     gene            13130..14377
                     /gene="icd"
                     /locus_tag="AC16HB_09325"
     CDS             13130..14377
                     /gene="icd"
                     /locus_tag="AC16HB_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR5607028.1"
                     /translation="MYQHIKVPKEGQKITVNKDFSLTVPDQPIIPYIEGDGTGIDITP
                     VMIKVVDAAVAKAYGGKRKIVWMEIYAGEKSTKIYGPDIWLPEETLQVLKEYVVSIKG
                     PLTTPVGGGIRSINVALRQELDLYVCLRPVRYFKGVPSPVKEPEKTDMVIFRENSEDI
                     YAGIEYEAESEKAKKVIRFLQEELGVKKIRFPGTSGIGIKPVSREGTERLVRKAIQYV
                     LDNRRKSLTFVHKGNIMKFTEGAFARWGYALAQKEFGAELLDGGPWMKIKAKHGDIVI
                     KDVIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIAPGANLSDSVAMF
                     EATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLVIRSMEKAILKKTVTYDF
                     ARLMEGATQVSCSGFGSAMIANM"
     gene            complement(14440..14646)
                     /gene="cspD"
                     /locus_tag="AC16HB_09330"
     CDS             complement(14440..14646)
                     /gene="cspD"
                     /locus_tag="AC16HB_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HIR5607029.1"
                     /translation="MATGTVKWFNDAKGYGFITPDGGGEDLFAHFSAIQMGGFKTLKE
                     GQKVNFEVVQGPKGKQASNITAAG"
     gene            15075..15383
                     /gene="clpS"
                     /locus_tag="AC16HB_09335"
     CDS             15075..15383
                     /gene="clpS"
                     /locus_tag="AC16HB_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR5607030.1"
                     /translation="MSERLGEGTVTEKKTSRVKPPPMYQVVLLNDDFTPMEFVVGVLQ
                     KFFGKGREQATQIMLKVHHEGRGVCGVYPRDVAASKVEQVCSYARQHQHPLQCVMEEA
                     "
     gene            15380..17692
                     /gene="clpA"
                     /locus_tag="AC16HB_09340"
     CDS             15380..17692
                     /gene="clpA"
                     /locus_tag="AC16HB_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR5607031.1"
                     /translation="MIAQELEVSLHMAFVEARQARHEFITVEHLLLALLDNPSAAEVL
                     RACACNIEDLRKSLQNFIGDNTPILPPNSEADTQPTLGFQRVIQRAIMHVQSTSNGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFISHGITKSPQGDEKAKQQGGEE
                     AQEQEQGREQASPLEQYTINLNQLAKDGKIDPLIGRELEVERVIQVLCRRRKNNPLLV
                     GEAGVGKTAIAEGLAWRVIKGDVPEILEKASVYSLDMGALLAGTKYRGDFEQRLKGVL
                     KQLKSNSNAILFIDEIHTLIGAGSASGGTLDASNLLKPALATGQLKCIGATTYNEYRG
                     IFEKDHALSRRFQKIDVVEPTVPQTIEILKGLKSRFEEHHGVKYSAGAITAAAELSAK
                     YINDRHLPDKAIDVIDEAGAAQRILPVSKRKKTIGKTEVEEIIAKIARIPPQTVSSDD
                     RTQLKNLERNLKNVVFGQDPAIDALAAAIKMARSGLGKPDKPIGAFLFSGPTGVGKTE
                     VAKQLAFTLGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAIAKKPHAVL
                     LLDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVIVIMTTNAGAETLNKRVIGF
                     SNTREAGDEMAEIRRMFTPEFRNRLDATISFRALDEEIILRVVDKFLMQLEDQLHEKK
                     VDITFSDELRKHLAKKGFDPVMGARPMARLIQDTIRRALADELLFGRLVNGGRVSVDV
                     DASGEIKLDFPPADGGDAPRAERPEPVTSE"
     gene            17817..18137
                     /locus_tag="AC16HB_09345"
     CDS             17817..18137
                     /locus_tag="AC16HB_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5607032.1"
                     /translation="MNHMPVTRSAAAVLLALAAAAASAQQPDPRSAPYLAATCTNCHG
                     TQGRSAGAMPSLAGQKKEYLAETMRAYRDGKRMATVMHQLAKGYSDAQIELIAEHFAK
                     QPAK"
     gene            18147..19439
                     /locus_tag="AC16HB_09350"
     CDS             18147..19439
                     /locus_tag="AC16HB_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HIR5607033.1"
                     /translation="MSMHNFKRRDVLKAGAATVAVTSLAACETLGGRKSLGQVVVIGA
                     GYGGATAAKYLRVWSEGAIDVTLVEPNEAFISCPMSNLVLGGSRTLTDITVAYSGLDK
                     YGVKRVRDSATGIDADNRQVRLASGATLSYDRLVVSPGIEFLWENVRGMSPAAAETVP
                     HAWKAGPQTVLLRRQLEAMADGGTVVMSAPLAPYRCPPGPYERACQIASYLKAHKPKS
                     KLLVLDANPDYVSKKPLFSRVFGEDYKGIIEYRTNAPVSEVDVAGKTFVLDMGDRIKG
                     DVLNLIPPQRAANIARDAGLITANNRWCEVDWTSMESVKVKGVHVLGDATLSAPLMPK
                     SGHMANQHGKAAAAAIVELLNGRSPIPPMMANTCYSYVDDKNAVHVASVHRYVPEKKT
                     LEVVAGASGISTQDRNSWALEGIFAWSWAQTIWADMLA"
     gene            complement(19446..20030)
                     /locus_tag="AC16HB_09355"
     CDS             complement(19446..20030)
                     /locus_tag="AC16HB_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5607034.1"
                     /translation="MLAASARWVLGVFGWRVDFNGMPGPKGIAIVYPHTSNWDFVIGL
                     LAKWVIAVPLYFVGKESLFRGLTGATLGRLMYLWGGRPVDRHHASGAVEQLAALMQKE
                     DFFWLALSPEGTRSYLPHLRTGFYYLALQLDLPVGLAYIDYARKRIGVTEFVRMTGDE
                     TRDLAMIRAYYADKVACYPEKASAIEFRDRASGA"
     gene            complement(20030..20476)
                     /gene="dut"
                     /locus_tag="AC16HB_09360"
     CDS             complement(20030..20476)
                     /gene="dut"
                     /locus_tag="AC16HB_09360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR5607035.1"
                     /translation="MKLEIKLLDARLRDSLPQYATAGSAGLDLRACIDEPTVLRAGDA
                     QLIPTGVAIHLADPAYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQIMVSCWNRGR
                     EPFTIRPFERVAQMVIVPVMQVELAVVDDFVESSRGAGGFGSTGKH"
     gene            complement(20473..20874)
                     /locus_tag="AC16HB_09365"
     CDS             complement(20473..20874)
                     /locus_tag="AC16HB_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607036.1"
                     /translation="MNLPAGEPKDGDFVAYLAQIERQQLAHLPSGAPTAPEGEAKDAA
                     AMPSQTATSTAQPATKPLPPALVGGLVLGFIGLFFLMVGLAGDGGWIAVAIGAFLLWR
                     AAKSLSAEARKPSKDVRALLAAKFPTGGKRG"
     gene            complement(20871..>21346)
                     /locus_tag="AC16HB_09370"
     CDS             complement(20871..>21346)
                     /locus_tag="AC16HB_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase
                     domain-containing protein"
                     /protein_id="HIR5607037.1"
                     /translation="ATPRGAQRVDVQTAREMHEAVMGYAHHTDVFIAVAAVADWRVAN
                     ASKTKLKKVRGAPPALAFEANPDILADVAALKNPPYCVGFAAESDDVIAYAQAKLESK
                     GVPLIVANRAQDAFGADDNELHLVDAVGVTTLPRDSKLAHARRIVAEIADRLRPR"
BASE COUNT         3772 a         6881 c         7086 g         3607 t
ORIGIN      
        1 gttcggcgct caggtgcgag caggcgaagg cgagaaacgg gcaacgggtg aactccggca
       61 attgagctac ggcagcttcc gatcgccgtc gccgagcggc cgctgcatca acacgctgtc
      121 cacccagcgg ccgaacttga agccgatcga cggcagcagg ccgacctgcg cgaacccgca
      181 ggcgcggtgc agttcgatcg agggcgcgtt ggcggaatcg ccgatcaccg ccagcatttg
      241 tcgaaagccg cgggcttcgc attcggcgat cagccgcacg agcaattgcc tgccgatgcc
      301 gcgtccggtc gcgtcgggcg cgatgtaaat cgaatcctcg accgagtatc gatacgcagg
      361 tcgcgtgcgg tagtgattcg cgtacgcgta cccgaccacg cggccgccgt catgcgcgac
      421 gagatatggg tagccgccgc gcgtgatcgc gacgtggcgt gaacgcatct cttcgaggga
      481 gggagcatcg agttcgaagc tcgccagtcc gtgcgtcacg tggtaggcgt agatgtcgcg
      541 gatcgccggc aggtcgccgg cgcgcacgga tcgaagttca ggggccatcg cggcaaacaa
      601 aacgggcggt gcggattgaa ccgcaccgcc cgtcgaaagt cacgcgcgca agcgcgtgca
      661 ggtttggtct atttcttttc ttcttccttc ttcgcctccg gtgctggcgc cggcgcaggg
      721 gccggcgcgg tcgtgggcgc cgtcgcgggt gcgacgacgg cgggcggggt caccggcatg
      781 gccgcgaccg gcttgccgcc gtggatcgac gcgatgatcg gcacgatcag cagcgccacg
      841 atgttgatga tcttgatcag cggattgacc gccgggccgg cggtgtcctt gtacggatcg
      901 ccgacggtgt ccccggtcac cgccgccttg tgcgcgtcgg agcccttgcc cccgtgatga
      961 ccttcctcga tgtacttctt ggcgttgtcc catgcgccac cgccggtggt catcgagatc
     1021 gcaacgaaca agccggtgac gatcgtgccc atcaagacgc cgcccaatgc ggcggggccg
     1081 aggacgatgc cgacgatgat tgggaccagc acgggcagga tcgacgggat catcatctcc
     1141 ttgatcgccg ccgtggtcag catgtcgacc gcgcgcgagt aatcaggctt cgcggtgcct
     1201 tccatgatgc ccttgatctc gcggaactgg cgccgtactt cctcgacgac gctgccggcc
     1261 gcgcgaccga ccgcttccat cgccatcgca ccgaacagga acgggatcag gccaccgatg
     1321 aacaggccaa tgatgacctt gtgatccgaa agatcgaagt tcgtgctcag gccgaaactc
     1381 ttcaggcctt cggtgtagtc ggcaaacaga acgagcgcgg caaggccggc cgaaccgatt
     1441 gcatagccct tggtcaccgc tttggtcgta ttgccgacgg cgtcgagcgg atcggtgatg
     1501 ttgcgcacct ctttgggcag ccctgccatt tcggcgatgc caccggcgtt gtcggtgatc
     1561 gggccgtagg cgtcgagcgc gacgatgatg ccggtcatcg acagcatcga cgtggctgcg
     1621 accgcgatac cgtagaggcc agcgagcccg tacgaggcgt agatcgcagc cgagatcgcc
     1681 agcacgggcc aggcgcacgc cttcatcgac accgcgagac cagcgatgat gttggtcgca
     1741 tgtcccgtgg tcgaggcttg cgcgatgtac ttgaccggcg tgaactgcgt gccggtgtag
     1801 tactcggtga tgtagacgag agccgcggtc agcacgaggc cgaccaccgc cgataggtac
     1861 aggttcatca ccgtgacgcc gccgctggcg gcgaccgggc cgaggatcca gttcgtcacc
     1921 ggccagaaca gcacgagcga aatgacgccg gcgacgatca gcccgcgata cagcgcattc
     1981 atgatcttgc cgccgtcgcg tgccttgacg aagaagcagc cgacgatcga ggccagaatc
     2041 gatacgcccg aaatcgccag cggatagatg atccccgcgt cgccgccgga cttgaagaag
     2101 aagccgccga tcagcatcgt ggcgaccacg gtgaccgcaa aggtctcgaa caggtcggcg
     2161 gccatgccgg cgcagtcgcc aacattgtcg ccgacgttgt ccgcaatcac cgccgggttg
     2221 cgcgggtcgt cttccgggat gccggcctcg accttgccga cgagatccgc gccgacatcc
     2281 gcgcccttgg tgaagatgcc gccgccgagt cgcgcgaaga tcgaaatcag cgaggcgccg
     2341 aacgcgaagc cgatcatcgg atgcagcagt tcgcgtgtgt tggccgccgc atgcgtggtc
     2401 ttcagcagcc agtagaaacc ggccacgccc aacaggccga gaccgacgac gagcaagccg
     2461 gtgatcgcgc cgccgcgaaa cgcgatgttc agcgcggcgt tcagcacctg ggtggccgcc
     2521 tgtgcggtgc gcacattgga acgcaccgac acgttcatgc cgatgaagcc ggccgcgccc
     2581 gacagcaccg cgccgagcac gaacccgatc gcggtccaca gcccgagttg cggcacgacc
     2641 gcgatcacga tcgcgagcac gactccgacg atcgcaatcg tgcggtactg ccggttcagg
     2701 tacgcctgcg cgccggtttg tatggccgcc gcgatctcct gcatccgcgg attgcccgcg
     2761 ggctgcttga ggatccagga aatcgacagt gcgccgaaca ccacggccgc gaggccgcag
     2821 agcagggcga gagccaatcc agacattacg ttctccttag tgtcggctga aacaagaacc
     2881 tactgggcga gcgcgtcgag cacccgttgc ttgatctcgc ccaccgtgcc cacgcccgac
     2941 acgcggacgt gcctcggtgc ggcggtgtct ccgccggccg accacttcgc gtagtagtcg
     3001 accagcacgc gagtctgcgc gtgatagacc tgcagccgct tgcgcacgac ttcctcgcgg
     3061 tcgtcgtcgc gctggatcag cggctcaccg gtggtgtcgt cgcggtctgg caccttcggc
     3121 gggttgaact tcacgtgata ggtgcggccg ctcgcgaggt ggacgcggcg gccactcatg
     3181 cgttcgatga tgtcggcgtc gggcacgtcg atctcgagca cgacatcgac cttgatgccg
     3241 gcggccctca gcgcctcggc ctgcgggatc gtgcgcggga agccatcgaa cagatagccg
     3301 gcggcacaat cgggctgctt cagtcgctcc ttgactagcg cgacgatgat gtcgtcggac
     3361 accagctggc cggcatccat caccctcttc gcggccacgc ctgtcggggt tcccgccttg
     3421 acggcgcccc gcaacatgtc gccggtcgag atctgtggga tgccgtagcg ctgcgtgatg
     3481 aacgcggcct gggtaccctt cccggcgccc ggcggcccaa gcaaaatgac tcgcatcccc
     3541 cgtcctgccc gtgaatttgt atccgacacg gccgcagccg gcggtatgtt cgcgcccgcg
     3601 ctggtcacgt catcggcgtg cgtctcgaaa ccggcgaatt ctaaccggtt cgccttgcgg
     3661 ggcgcttacg ccgagtgtga cgggactatg cgcccgatca aatcttgaac acgttcggca
     3721 ggcgtttctt taccgcaacg ttcttcagca ccgcatagtc gggcagaccg tccttgtacg
     3781 gcggataggc ctcgccgcgg atcagcggcg agaggtagcg gcggcacgct tccgtgatgc
     3841 caaagccgtc cgcgctgatg aattcgcgcg gcatcgtctt ctcgcggttc gcgacgtcgg
     3901 cgagcggcgc catttcgatc ttccagcgat acggccggct cgaggtgcgc acgatggccg
     3961 gcatcactgc gttgtggccc ttgagtgcaa actcgaccgc ggccttgccg acggcgtacg
     4021 cctgctcgac atcggtcttc gacgcgatgt ggcgcgcggc gcgttgcagg tagtcggcca
     4081 ccgcccagtg gaacttgtag ttcaggctct ccttgaccag gcttgcgatc accggcgcca
     4141 ccccgcccaa ttgcgcgtgt ccgaatgcgt cgcgcaggcc ttggtccgac aggaacttgc
     4201 cgtccgtgcc cttgacaccc tcggacacga ctaccgagca gtagccgagg cgttcgacct
     4261 tgtccttcac cgcggcaagg aatccgtcgc gatcgaaggg cacctcgggg aacaggatca
     4321 cgatcggaat cgggtgcgtc ctgtcggagg ccagcccgcc cgcggccgcg atccagccgg
     4381 cgtggcgtcc catgacctcg aggacgaaca ccttggtcga ggtcttcgcc atcgaagcga
     4441 cgtcgaaact ggcctcgcgt gtcgacaccg caatgtactt ggccacggac ccgaagccgg
     4501 gacagacatc ggtgatcggc aggtcgttgt cgaccgtctt cggcacgtgg atcgcctgca
     4561 gcggatagcc gagtttgtcc gacagctgcg acaccttcag gcacgtgtcg gccgaatcac
     4621 cgccgccgtt gtagaagaag tagccgatat cgtgggcgcg gaacacctcg atcagacgct
     4681 cgtactgcgc gcggctttcc tccagaccct tgagcttgta gcggcacgag ccgaacgcgc
     4741 cggcgggcgt gtgtttcagc gccttgatcg tcgccgcgga ttcgcggctg gtgtcgatca
     4801 ggtcttcggt caacgcgcca atgatgccgt tgcggccggc gtaaaccttg ccgatacggc
     4861 tcttgtagcg gcgtgcggtt tcgatcacgc ccgccgcgga agcattgatg acggcggtca
     4921 cgccgccgga ctgtgcataa aaggcgttgc gcttcttcat ttcgatgggg aaccgttctc
     4981 gaatgaaacc attgtgtctt cgcccggcgc tgcgcgcttc aacttcgtcc gttccgaagt
     5041 cagccggcga cgcgcttgtt tgataagcgt tcagcgcttg tcaatcgatc cgcgtccatt
     5101 cgcccatcaa ccagttgtcg gcacgatgtg tcacgtgaac attggcccgc atcgcccacg
     5161 ggatcacctg ccggtgcaac ggcacgtggt gcacctgtgc attgtgctcg gcaagcgcct
     5221 ggcgaatgat ttcggcgcgc ttttccgcgt tcatctcgat tgcggcggcg tcgatcagcg
     5281 cgtccagctt ggggttggcg tagcggccgt agttgaaact gccgttgccg cttttcggat
     5341 cgaacgagcg cagcaccggc gacagcgtcg tctgcgcgtc ggtgatcgca ccgccccagc
     5401 cgagcatgta gaagctggtg tcgagcttct ccagcttcgg aaagtacgtc gcgcgcggct
     5461 gtgcggtgac cctcgtctgg atgccgacct tggacagcat tgccgccacc gcgaggcaga
     5521 tcttctcgtc gttgatgtag cggttgttcg gacagtcgag cgtgacttcg aagccattgg
     5581 gatagccggc ctcggcgagc agtcgcttcg cctcgctgag gtcgtacggc aggcgcttct
     5641 cggcctcggg gttggaggcc agaatcgacg gcgtgatgcc gccggtgggc agcgcggcgc
     5701 cgcgcatcgt gcgtgatcgc agcgcctcga tgtcgattgc ctggtagacc gcgcggcgca
     5761 cgcgcgcgtc cttgaacggg ttcctgccct tgacattcga gtacagcaac tcgtcgcgcg
     5821 cctggtcgaa gccgaagaac accacgcggt tctccatccc gtcgacgacc ttgacgcccg
     5881 cggtcgcgcg cagccggtcg agatcctgcg gcggcgggtc gaggatgaag tcgacctgcc
     5941 ccgacagcag cgccgccacg cgtgtggcgt cggaggcgat cggcgtgtag acgatctcgt
     6001 cgacgttgcc ctcgaacgtg ccccaccagt tcggattgcg cttcagcgct gtcttcacgc
     6061 ccggttcccg caccgccagc atgtacgggc cggtgccgtt tgcattgcgc gcggcgaaag
     6121 tctcttcgcg gtccttgaat gacagcggtc gctcgacctt gtgcttctcg gaccacgcct
     6181 tgctcatgat gaagatcgtg gcgacgtgct gcagcatcac cgggttcggc ttgtcctgca
     6241 cgaactcgac cgtgtagtcg tcgattttgc gcggggcgcc ggtgacgggg atcgcgtact
     6301 ggcggatctg cgagttggcg tggctggcac gctcgaacga gaacacgacg tcgtcggccg
     6361 tgaacggcgt gccgtcgtga aacttcactc ccttgcgcag gttgaagcgc cacgtcagcg
     6421 gtgacggctg cgaccagctg accgccaggc cgggaacgat tcgcagttcc ttgtcccgcg
     6481 tgaccagcgt gtcgtgcacc tgctgcgaca gcagattcgt cagcagttcg ttctgcgagt
     6541 acggatctgc ggtctgaaag tcaccttgcg atgcccagcg cagggtcttg gcgccagcgc
     6601 cgctcacgac gagcagcagc gcggcgaggg cggcatacca gcgattcatc cgtgtccgtc
     6661 gagaagtccc ggtgcgcgcg agtctatgcg cggccagcaa tcagtgcacg cacgtgctcg
     6721 agatcctcga aggtgtcgac gcccggaggc aacggggcgt cgagccgtag taccgcgatg
     6781 gcgtggccgt gccacagcgc gcgcagttgt tcgaggcttt cgtgcgcctc tatcggggcc
     6841 ggtggcaggt gcggatagcg gcgcaggaac ccggcgcggt aagcgtacag gccgacgtgg
     6901 cgcaacgcgg gcagtccgtc gggcaggcgc ccgcgcgtct gtgcgaacgc gtcgcgcgac
     6961 cacgggatcg gcgcgcgcga aaagagcagc gcgcgtccgc gcgcatcggt gacgaccttc
     7021 acgacgttcg ggctgaagaa ctcggctgct tccgccaccg gatgtgcgac cgtggccatc
     7081 gcgcagtcag gctgcgcctg cagtcgcgcc gccacggcgt cgatcactgc cggagggatc
     7141 agcggctcgt cgccctgcac gttgacgacg atcgcgtcgt cgggcagcgc aagttgctgc
     7201 gcggcttccg caacacggtc agtgccggtc gggtggcgcg tcgacgtcag caccgccgac
     7261 acaccctgcg cggcacacgc gcgcgcgatc tcttcgctgt cggccgcgac gatcacgcgt
     7321 tgggcgtggc tttctgccga tcgctgcgcg acccgcacga tcagtggcag gccaccgacg
     7381 tcggcgagca tcttgcgcgg cagccgcgtg gaagcgaggc gtgccggaac gatgacgacg
     7441 aagccggcca tcgcgagtga tcgacgcggc tcccgctcga gccccggtcg cggctcccgc
     7501 gtcgtcgagc cgccgtgcct cgctttcgag catgaccgga atgccgtcgc ggatcgggta
     7561 ggcgagtcca gcgctgcgcg agatcagttc ctgccgctcg cggtcatatt cgagcgggcc
     7621 cttggtgacc gggcagacga ggatgtcaag cagtcgtgga tccatgtgcg gcttcgtgtt
     7681 gcacggcggg cgaagccagc cgcatttcga tcagatcgat caacccggca tcgatcgtcg
     7741 cctgcaatcc gaccacgcac acgcgtccat cggtggcgat cgccgagacc gcgcggcatt
     7801 ttacggcgtc tttcgcggtg atcagcgcca ggtcgaactt gcggccgaga aagggattca
     7861 ccgcgtaatc gtagtggtcc ggcagcggca gttcttcgat gtcgagcccg gcggcgcgca
     7921 gcatcccgaa gaagcgctcc ggtgcgccga tgccggcggc ggcgatgatc ttcagctggc
     7981 cgcgccgctg ttcctgcacc agtccttcga gcgagatcat gcgcgtggga tcggcgagcg
     8041 aacgcacgtc gccgagacgg ctcaccatct cgtagcgtgg cgaatacacg cgcacgggcg
     8101 gcacgatgcc gtgacagacg acggcggcga cctccttcag gcgcgccggt ggatcgcgca
     8161 acggacccgc cggcagcagc cagccgttgc ccagcccgcg ttcgtcgagc aatgcgatct
     8221 cgaaatcgcg cgccagccgg tggtgctgca ggccgtcatc ggcgacgatc acgtcgcagc
     8281 gggggtggac gctgcgcagg atccggcatg ccgcgacacg atcgcgcccc acgaccaccg
     8341 ggacaccctg gccctgcagc gcttgtgcca gcagcagcgg ctcgtcaccg acctcggtcg
     8401 cgcgggcatg ggtctcgacc gtgcggacgt cgtttccgcc ggcaccgtaa ccgcgcgtga
     8461 cgacacccgg gcggcggccg cggagcagca gcgcgcgaac cagttcgagt gtcaacggtg
     8521 tcttgccggt gccgccgacg tagaggttgc cgaccacgat caccggcacg ccgacgtgcc
     8581 aggcgcgccg cacgccgatc cgatagctcc aggcgatcag tgcgcgcacg gcgccgtaca
     8641 cgagcgccag cggcagcagc agccacgccg cggggccacg cgtgcgccac actcggtgca
     8701 gcgtgcgttc cagccgcgcg gtgaggtcgg gccggttcaa ctcattgcgc agccgtgttc
     8761 tgccgctcgg tgacaaagct gatgcgcgac aactgggcaa tacgcgcggc ttccatcacg
     8821 ttgatcaccg actgatgcgg cgactgccca tcggcgtgga tcacgaccag caggtcggtg
     8881 cgtccctgcg cggccgcgcg cagtgcctcg accagcgtgt cgacgtcgga cttcgccagc
     8941 acgcgcttgt cgatcgcgta gcgaccgtcc gcggtgacgc cgatcacgac ttcgttcggc
     9001 cggttctgcg tggccttggc ctcggcgcgt ggcagttcga ttttcagctc ggcgaaacgt
     9061 gagtaggtgg tggtgaccgc gagaaagatc agcacgatca gcatcacgtc gatcagagga
     9121 atcaggttga gctctggttc ctcgtcgcgg gcgcgccgga atttcatcgc ggggtcgcgt
     9181 cgtagcggtc ggcgaacagg acttcgagca gtcgctgcga ctggtcctcc atctcgacca
     9241 ggaagtcctc gatgcgcgaa cggaagtggc ggtagaaaag caccgatggc acggcgacga
     9301 tgatgccgaa cgcggtgcag tacagcgcga tcgaaatgcc gtgcgcaagc tgctgcggat
     9361 tggagccggc ctgctgcgag gcaaagatat cgatcattcc gatcacggtg ccgagcaatc
     9421 ccagcagcgg cgcgaccgaa ccgatcgatc cgagcagcga caggtagcgc gacaggtcgt
     9481 gcgcgatgtg gccgcccgtg ttcgcgacgg ctgcccccgc gattgcgcgc ggcgcgcgca
     9541 cgtttcgcaa agcggtcgcg agcacgcgtc cgagcggcga gttgcgctcg agcttcagga
     9601 tcacctcgga attgacctgt cgtcgttcaa ccaggttcag cacgtcctgc aggagcgact
     9661 ctggaatcac cttgccgcgc cgtagcgcga tgctgcgctc gatgatgagg gtcagggcga
     9721 cgacggaagc cgcgagcaac ggccagattg gccaaccgat ggcctgtacg atcgcaaaca
     9781 agtccgtgcc cctccgtccc gtttcactcc ggacgcggag tgtagtcggt gggccggatg
     9841 acgcagcgac ggcatctgga ctagcgaact ttccccattt tctgtggata agtttgtgaa
     9901 caacagtgga tgatggctcg gcaaaggacg aaaacacgcg gtccggccca attgcacgcg
     9961 gcttgagcaa gacagaatct gtcgacgaat catgtgctta cacctatctg tcggcggcgg
    10021 cacgggtttc ggcccgcacg agcaaagtat ggcgcgccaa tgtggacagt tgtcggccgc
    10081 gaacccgcat gacgcctgaa ttcgcggacg cgaacctagc aacgacgcgg gatccgctga
    10141 cggtttccgc gctgaaccgc gtcgttgctg ggttgctcgc gcgcgccttc ccgctggtgc
    10201 gggttcgcgg cgaaatcgcc aatttcaccc gtgccgccag cgggcactgg tacttcacct
    10261 tgaaggatcc gggagcgcag gtccggtgcg cgatgttccg cggccgcaat gggctggtcg
    10321 atttcgcgcc gcgcgaaggt gaccacgtcg acgtgctggc gcaggtaggc ctgtacgagg
    10381 cgcgtggcga atttcagctc aacgtcgagt cgatgcaacg cgcgggactc ggacgtctgt
    10441 acgaggaatt cctgcgcctg aaggctcgac tggcggccga gggagtcttc gatgaagccc
    10501 agaagcgccg gctgccgtta ttgccacgcg cagtcggcat cgtgacatcg ctgcaggccg
    10561 ccgcgctgcg cgatgtcctg accacgctgc gccggcgcgc gccgtatctg cggctgatcg
    10621 tctatccggt gcccgtgcag ggcgagggcg ccggtgcacg tatcgcgcaa atgctgcaga
    10681 gagtctccgc gcgcgccgag gtcgacgccg tgctgctggt gcgcggcggc ggctcgatcg
    10741 aagacctgtg gtccttcaac gaggaacccg tggcgcgcgc aatccgcgcg tcggcgctgc
    10801 cggtggtcgt gggtgtcggg cacgagtccg acttcacgat tgccgatttc gcggccgatc
    10861 tgcgcgcacc aacgcccaca gccgcggccg agctggtggc gccggatcgc agcgggctgg
    10921 tcgattccgt gacgcagaag ctgcgctcat tgcgggtagc gttgcgacgg cgcaacgaga
    10981 tcgcgcagca gcggttcgac tatgcgttgc ggtcgctggc agtgccgcgc gcccccctgc
    11041 gcgcgctcga ggcgcgcgtt gccagcgctg tcacacagct gcgccatctg gtcgcgagaa
    11101 ccgcggttgc caggcgcgcg gcgaccgcgc gctgcctgca gcgactttcg cggcggcgtc
    11161 cggatgcgct cttgcggcgg gcgcggctgc tgcaagcgtg cgcgcggctg tgcgcgtctg
    11221 cgcagcgaca acacaaatcg cggcaaggcc ggttcgaggc gctcgccgcc aagttgggcg
    11281 cgctcgatcc gcacgctgtg ttggtgcgcg gttacgcgat ggcggtggcg ccggacgggc
    11341 gcgtggttcg cgatgcacgt cagctcgcgc cgggcgatcg cctgcgcctg cagtttgcgc
    11401 gcggcggtgc gcacgcgcgg gtggaagacc ttgaactgcg cgcgggcaac accgatgcgc
    11461 cagatgacta gaatgccgcc ctctgccata gagctgcgat cgtcctcaac tctccgcgaa
    11521 ggaagcacgt catggaacac acgctgcccc cgctgccgta cgcaatggat gcgctcgcgc
    11581 cgcacatctc gaaggagacc ttcgagtatc actacggcaa gcaccatcag gcctacgtga
    11641 ccaacctgaa caacctgatc aagggcaccg aattcgaagc gagttcgctc gaggacatcg
    11701 tgaagaaatc ctcgggaggt atcttcaaca acgccgctca ggtgtggaat cacacgttct
    11761 tctggaacag catgaagccc ggcggtggtg gcgagccggc cggtgcgctt ggcgcggcga
    11821 ttgccaggaa gtgggcctct tacgccgcct tcaaggaagc gttcgccaag agcgccgtcg
    11881 gcaactttgg ctctggctgg acgtggctgg tgaagaaggc ggacggctcg gtcgaaatcg
    11941 tcaatacgag caatgcggcc acaccgctga cgaccgcgga cagggcgctg ctgacgatcg
    12001 acgtctggga gcacgcttac tacatcgatt accgcaatgc gcggccgaag ttcgtcgaga
    12061 cgttcctgaa caatctcgcc aactggggat tcgcggcgaa gaatttcgcc tgatcttgcg
    12121 cgcggggccg ggcatcggtc tcccgcgaac cggaacgtca gcgcgggcgc ggcagaccgt
    12181 tgatcttcgc gccgaccttc agtccgcgct tgtcgaacca accgcgctcc atttcaagcg
    12241 cgaatcgcac cggcttgctg gagcaatgct gattctcggt ccggggtgcc atgtcctcga
    12301 ggtcgacaat cgttccgtca tcgtcgatga aggcgattgt cagtgggaca agcgtattgc
    12361 gcatccagaa gcacatcggc gatttcgcac ggtacacgaa gagcatgccg tgattgggtt
    12421 ccagccgttc acggaacatc agtccacgtg tggtcgtgac cggatcggcc acgacttccg
    12481 ccgtgatcag gtggatcccc acgttgagct tgatcgccgg ctgcggtggt aacggtccct
    12541 gcgcgccagc gaggatcgat accagtgcga ggcaaactcc aagcagggcg cgaactagct
    12601 ttttcatggc gtctcgggct ttcaatagaa aaagggcagg gccttggagc cctgcccctt
    12661 catccgtgac cgacaaagtt acttcttctc ggcttcggct ttcttggctt tcctggcttt
    12721 cttgactgcc tttgctttct tcacttcctt ggcttcggcc ttggcttccg ctttcggcgc
    12781 ggccggggcg gcggaaggcc ctgcaaccgg ggtcgccggg gttgccggcg tcgccggctt
    12841 ggccggagcg gcgggggcag cctgtgcaaa gacggcgcca gcgaacaagg ccgagatcag
    12901 agcagcgatc gctttcttca tggaactccc tttcggtcgt ttggggttgt gcgacgaccg
    12961 gaagtccggc cgcggacggc gttgcgtcag agcccgtaac gcgcccggcg aggaaccggt
    13021 tgacgggccg gggacggcgg gcgagtaaca tcgtggcgaa acgacgctcg actcgggctt
    13081 tcaccgcgcc ggcggccagc gccggaatcc ttctcagtcg gagaccccca tgtatcagca
    13141 catcaaagtg cccaaggaag ggcagaagat caccgtcaac aaggatttct ccctgactgt
    13201 cccggatcaa cccatcatcc cgtatatcga gggcgacggc accggaatcg acatcacacc
    13261 ggtgatgatc aaggtggtcg acgcggccgt ggccaaggcc tacggcggca aacgcaagat
    13321 cgtgtggatg gagatctacg ctggcgagaa gtcgaccaag atctacggcc ccgatatctg
    13381 gctgccggaa gagaccctgc aggttctcaa ggagtacgtg gtgtcgatca aggggccgct
    13441 gacgaccccg gttgggggcg ggatccgttc gatcaacgtc gcgctgcgcc aggaactgga
    13501 cctgtatgtg tgcctgcggc ccgtgcgcta cttcaagggc gtgccgagcc cggtgaagga
    13561 gccggaaaag accgacatgg tgatctttcg cgaaaactcc gaggacatct acgccgggat
    13621 cgaatacgag gcggagtccg agaaggcgaa gaaggtgatc cgcttcctgc aggaggaact
    13681 gggggtcaag aagatccgtt tcccgggcac gtccggcatc gggatcaagc cggtgtcgcg
    13741 cgaaggcacc gagcggctgg tgcgcaaggc gatccagtat gtgctggaca acaggcgcaa
    13801 gagcctgacc ttcgtgcaca agggcaacat catgaaattc acggaaggcg cgttcgcccg
    13861 atggggctat gcactggcac agaaggaatt cggcgccgaa ctgctcgacg gcgggccatg
    13921 gatgaagatc aaggccaagc acggcgacat cgtcatcaag gacgtgatcg ccgatgcgtt
    13981 cctgcaacag atcctgctgc ggcccgccga atacgacgtg atcgccacga tgaacctgaa
    14041 cggcgactac atctccgacg cgctggcggc gcaggtcggc ggtatcggca tcgccccggg
    14101 cgccaatttg tcggattcgg tggccatgtt cgaagcgacc cacggcaccg cgccgaagta
    14161 cgccggcaag gactacgtga atccgggctc ggagattctg tcggcggaaa tgatgttgcg
    14221 gcacatgggc tggaccgagg cggccgacct ggtgatcagg tcgatggaga aggcgatcct
    14281 gaagaagacc gtcacctacg acttcgcgcg cctgatggag ggcgccacgc aggtctcatg
    14341 ctctgggttc ggaagcgcga tgattgccaa catgtgaatg ccgcggtcgc cacaacaaaa
    14401 agccccgcct cggcggggct ttttgttggc gtgtcgagtt caaccggcgg cagtgatgtt
    14461 cgacgcctgt tttcctttcg gaccctgcac cacctcgaag ttcactttct ggccctcctt
    14521 cagggtcttg aagccgccca tctggatcgc cgagaagtgg gcgaacagat cttcgccacc
    14581 cccatcgggt gtgatgaaac cgtacccttt ggcgtcgttg aaccacttca ccgtgcctgt
    14641 tgccatctgt tcttcctttt cgtacgtctc aaactggttg gtgccgactc ctgcgagcgc
    14701 actcccaccc ccgctgcggc gttcgtccga gtcgttcgac ccgccggccg ccaggccggc
    14761 cctgcccacc gggcatggca cggccgcgca ttctttgggc aaattcgggc tggcgtcaag
    14821 gtccggcgcg gcgagaccgt cgccagcggc gacccgggcg gacgaccggg catggccccg
    14881 ccagtgggcc tcgcgcttgt gtttcgcgct cgccgtctcc actttgagaa acggtaggac
    14941 ccagtgaagg cacgactcat gcgcagcgct ggcggccgat ctgaatggcc gaggccggca
    15001 gtccccggag ttaacttatg attaggacgc tttgatggtc tgacggggaa cggcgccttg
    15061 cctagaatgg ttccatgagt gagcggctgg gtgaaggtac agtcaccgag aagaagactt
    15121 ctcgggtgaa gccgccgccg atgtaccagg tggtgctgct caacgatgat ttcacgccga
    15181 tggaattcgt ggtcggcgtg ctgcagaagt tcttcggcaa gggccgcgag caggcgacgc
    15241 agatcatgtt gaaggtacac cacgaaggtc gcggcgtctg cggtgtctat ccgcgagacg
    15301 tggcagccag caaggtggag caggtgtgca gctacgcacg ccagcaccag catccgttgc
    15361 aatgcgtgat ggaggaagca tgatcgcgca ggaacttgaa gtcagcttgc acatggcctt
    15421 cgtcgaggcc cgtcaggcgc gacacgaatt catcacggtc gagcacctgc tgcttgcgct
    15481 gctggacaat ccgtccgcgg ccgaggtgct gcgcgcctgt gcgtgcaaca tcgaggatct
    15541 gcgcaagagt ctgcagaact tcatcggtga caacacgccg attctgcccc cgaactcgga
    15601 ggccgacacg cagcccacgc tcggcttcca gcgtgtgatc cagcgcgcga tcatgcacgt
    15661 gcagtccacc agcaacggca agaaggaagt cacgggtgcc aacgtactgg tggcgatctt
    15721 cggcgagaag gactcgcacg cggtgtacta cctgcaccag cagggcgtga cacggctgga
    15781 tgtcgtgaac ttcatttcgc acggcattac caagtccccg cagggcgacg agaaggccaa
    15841 gcagcaaggc ggcgaagaag cgcaggagca ggagcagggc cgcgagcagg cctcgccgct
    15901 cgagcagtac acgatcaacc tgaaccagct cgccaaggac ggcaagatcg atccgctgat
    15961 cgggcgcgag ctcgaagtcg agcgcgtgat ccaggtgctg tgccgccgcc gcaagaacaa
    16021 cccgctgctg gttggcgagg ccggcgtggg caaaactgcg atcgccgaag gcctcgcgtg
    16081 gcgcgtgatc aagggcgacg ttccggagat cctcgaaaag gcctccgtct attcgctcga
    16141 catgggcgca ttactggccg gcaccaagta ccgcggcgat ttcgagcagc ggctcaaggg
    16201 cgtattgaag cagctcaaat cgaactccaa cgcgatcctg ttcatcgacg agatccatac
    16261 gctgatcggc gccggctccg cttcgggcgg cacgctggat gcatcgaacc tgctgaagcc
    16321 ggcactcgcg accgggcagc tgaaatgcat cggcgcgacg acttacaacg agtaccgcgg
    16381 catcttcgag aaggatcacg cgctgtcgcg ccgcttccag aagatcgacg tggtcgagcc
    16441 gacggtgccg cagacgatcg agatccttaa gggcctgaag tcgcgcttcg aagagcacca
    16501 cggggtcaag tactcggcgg gcgccattac ggccgctgcc gaattgtcag cgaagtacat
    16561 caacgaccgc cacctgccgg acaaggcgat cgacgtgatc gacgaggcgg gcgcagcgca
    16621 gcgcatcctg cccgtcagca agcgcaaaaa gacgatcggc aagaccgagg tcgaggagat
    16681 catcgccaag atcgcgcgca ttccgccaca gacggtctcc agcgacgatc gcacgcagct
    16741 gaagaacctc gagcgcaacc tcaagaacgt cgtgttcggc caggatccgg cgatcgacgc
    16801 gctggccgcg gcgatcaaga tggcgcgctc ggggctgggc aaaccggaca agccgatcgg
    16861 cgcgttcctg ttctccggcc cgaccggcgt cggcaagacc gaggtcgcaa agcagctggc
    16921 attcacactg ggtatcgagc tgatccgttt cgacatgtcc gagtacatgg aacgccacgc
    16981 cgtgagccga ctgatcggcg cccccccggg ctacgtcggc ttcgaccagg gcggcctgct
    17041 gaccgaggcg atcgccaaga agccgcatgc ggtgctgctg ctcgacgaga tcgagaaagc
    17101 gcacccggac atcttcaaca tcctgctgca ggtcatggat cacggcacgc tgacggacaa
    17161 caacggccgc aaggccgact tccgcaacgt catcgtgatc atgacgacca acgcgggcgc
    17221 ggaaacgctg aacaagcgcg tgatcggttt cagcaacacg cgtgaagcgg gtgacgagat
    17281 ggcggagatc cggcggatgt tcacgcccga gttccgcaac cggctcgacg ccacgatcag
    17341 cttccgcgcc ctcgacgagg agatcatcct gcgcgtggtc gacaagttcc tgatgcaact
    17401 cgaggaccag ttgcacgaga agaaggtcga catcacgttc tccgacgagc tgcgcaagca
    17461 cctggcgaag aagggctttg acccggtgat gggcgcacgg cccatggcgc gtctgatcca
    17521 ggacacgata cgccgcgcac tggccgacga actgttgttc gggaggttgg tcaacggcgg
    17581 ccgggtcagc gtggatgtcg atgcgagcgg cgagatcaag ctcgacttcc cgcccgccga
    17641 cggcggcgat gcgccgcgcg cggagcgacc cgagccggtc acgtccgaat agtcatactc
    17701 ccctcggggc ggattgacag tccgccgcga tgggtaaaca gtacggcccg caggggaatt
    17761 cccctcgcgg gccgttttct ttggcggcca ttacgacaaa tcgaggagag acagaaatga
    17821 accacatgcc ggtcacgcgc agcgcggccg cggtgttgct cgcgctcgct gcagccgccg
    17881 cgtccgcaca gcaacccgat ccgcgatccg caccgtacct cgccgccact tgcaccaact
    17941 gtcacggtac gcaaggacgg agcgctgggg cgatgccgag tctggctggg cagaagaagg
    18001 aatacctggc cgagacaatg cgcgcgtatc gagatggcaa gcggatggcc acggtcatgc
    18061 accagcttgc caagggttac agcgacgcgc agatcgaact gatcgccgaa cactttgcca
    18121 agcagccggc caagtaagga gacgccatgt caatgcacaa cttcaagcgg cgcgatgtgc
    18181 tgaaggccgg tgccgccacg gtcgcggtga cgtcgctggc cgcgtgcgag accctggggg
    18241 gccgcaaatc gctcggccag gtggtcgtga tcggcgccgg ctatggcggt gccaccgcgg
    18301 ccaagtatct gcgcgtgtgg tccgagggag cgatcgacgt cacgctggtg gagcccaacg
    18361 aggccttcat ctcctgcccg atgtcgaacc tcgtgcttgg cggcagccgc acgctcaccg
    18421 acatcacggt ggcttacagc ggtctggaca agtacggcgt gaagcgcgtg cgcgacagcg
    18481 ccacgggtat cgacgccgac aaccgccagg tgcggctcgc gtcgggagcg accctgagct
    18541 acgaccgact ggtcgtgtcg cccggcatcg agttcctgtg ggagaacgtg cgcggcatgt
    18601 cgcccgcggc ggccgagacc gttccgcacg cttggaaggc cggtccgcag accgtgctgc
    18661 tgcgccggca actggaagcg atggccgacg gcggcacggt ggtgatgagc gcgccgctgg
    18721 cgccgtaccg ctgcccgccg gggccatacg agcgcgcctg ccagatcgcc tcgtacctga
    18781 aggcgcacaa gccgaagagc aagctgctcg tgctggacgc caatccggat tacgtttcca
    18841 agaagccgct gttctcgcgc gtgttcggcg aggactacaa gggaatcatt gaatatcgca
    18901 ccaacgcgcc ggtgagcgaa gtcgacgtgg cgggcaagac cttcgtgctg gatatgggtg
    18961 accgcatcaa gggcgacgtg ctgaacctga tcccgccgca gcgcgcggcc aacatcgcgc
    19021 gcgacgccgg gctgatcacg gccaataacc gctggtgcga ggtcgactgg acttcgatgg
    19081 agtcggtcaa ggtcaagggc gtgcacgtac tcggcgacgc taccctgtcc gcaccgctga
    19141 tgcccaagag tggccacatg gccaaccagc acggcaaggc ggccgccgcg gccatcgtcg
    19201 aactgctcaa cggcaggagc ccgatcccgc cgatgatggc caacacctgc tacagctacg
    19261 tcgacgacaa gaacgccgtg cacgtggcca gcgtgcaccg ctacgtgccc gaaaagaaaa
    19321 cgctcgaggt ggtcgccggc gccagcggca tctctaccca ggaccgcaac tcctgggccc
    19381 tcgagggcat cttcgcctgg agctgggccc agaccatctg ggccgacatg ctggcctagg
    19441 atcgctcagg cgccggaagc gcggtcgcgg aactcgatcg cgctcgcctt ttccggatag
    19501 cacgcgacct tgtccgcgta gtacgcacgg atcatggcca ggtcgcgtgt ctcatcgccg
    19561 gtcatccgga cgaactcggt gacgccaatg cgcttgcgtg catagtcgat gtacgccagc
    19621 ccgacgggaa ggtcgagctg caacgcgaga tagtagaagc cggtgcgaag atgcggcagg
    19681 tagctgcgcg tgccctcggg tgacagcgca agccagaaga agtcttcctt ctgcatcagc
    19741 gcggccagct gctcgaccgc gccgctggcg tgatggcggt cgaccgggcg cccgccccac
    19801 aggtacatca gtcgaccgag cgtggcgccc gtgaggccgc ggaacaggct ctccttgccg
    19861 acgaagtaca gcggcaccgc gatcacccac ttggcgagca acccgatcac gaagtcccag
    19921 ttcgacgtgt gcgggtaaac gatcgcgatg cccttcggcc ccggcatgcc gttgaagtcg
    19981 acgcgccatc cgaacacgcc gagaacccag cgcgcggacg cggccagcat cagtgcttgc
    20041 cggtcgagcc gaatccgccc gcaccgcggc tgctctcgac gaaatcgtcg accaccgcga
    20101 gttcgacctg catcaccggc acgatcacca tctgcgcaac gcgttcgaac ggccgaatcg
    20161 tgaacggctc gcggccgcgg ttccagcacg acaccatgat ctgcccctgg taatcggagt
    20221 cgatcaggcc cacgaggttg ccgagcacga tgccgtgctt gtggcccagc cccgagcgcg
    20281 gcaggatcag cgccgcgtac gcgggatcgg cgaggtggat cgcgacgccg gtcgggatca
    20341 gttgcgcgtc gccggcgcgc agcacggtgg gttcgtcgat gcacgcgcgc aggtcgaggc
    20401 cggccgagcc cgccgtcgcg tactgcggca gcgagtcgcg cagccgtgca tcgagcagct
    20461 tgatctcgag cttcatccgc gcttgccgcc ggtggggaac ttcgcggcca gcaacgcgcg
    20521 cacgtccttt gacggcttgc gcgcctcggc ggacagcgac ttcgccgcgc gccacagcag
    20581 gaacgcgccg atggcaaccg cgatccagcc gccgtcgccg gccagtccca ccatcaggaa
    20641 gaacaggccg atgaatccga gcacgagccc gccgaccagc gccggcggca gaggcttcgt
    20701 agctggctga gcggtcgagg ttgccgtctg gctcggcatt gcggcggcgt ccttcgcttc
    20761 gccctcgggc gccgtcggcg cacccgacgg caggtgcgca agctgctgcc gctcgatctg
    20821 cgccaggtac gcgacgaagt cgccgtcctt cggttcgccg gcgggcaggt tcatcgtggt
    20881 cgcagcctgt ccgcgatctc ggcaacgatg cgccgtgcgt gcgcgagctt gctgtcgcgc
    20941 ggcagcgttg tcacaccgac tgcgtcgacc aggtgcagtt cgttgtcgtc ggcaccgaag
    21001 gcgtcctgcg cgcggttggc gacgatcagc ggcacgccct tggattcgag cttcgcctgc
    21061 gcgtaggcaa tcacgtcgtc gctctcggcc gcgaagccaa cgcagtacgg cggattcttc
    21121 agcgccgcca cgtcggccag gatgtcgggg ttcgcctcga acgcgagcgc aggcggcgcg
    21181 cccctgacct tcttcagctt ggtcttgctt gcgttggcca cgcgccaatc cgcgacggcc
    21241 gccacggcaa tgaacacgtc cgtgtgatgc gcgtagccca tcaccgcttc gtgcatctcg
    21301 cgcgcggtct gcacgtcgac gcgctgcgcg ccgcgcggtg tggcga
//
LOCUS       DBNHBY010000376         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1240925, whole genome shotgun sequence.
ACCESSION   DBNHBY010000376 DBNHBY010000000
VERSION     DBNHBY010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1240925"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..518)
                     /locus_tag="AC16HB_09375"
     CDS             complement(78..518)
                     /locus_tag="AC16HB_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607038.1"
                     /translation="MAAARRIPDPQAPKGNDMKRTRSTVRAACTTTLAIALLTIGAAP
                     LRADPIQAAYLQSVRAHHALWHAAAIDDFLAHRYAIAYGRFALLADAGHVPSASVALM
                     MVRHDPSLFGAAWPATPEQLQRWSAMVAADLREHGAPRTEHDGE"
     gene            650..>2375
                     /locus_tag="AC16HB_09380"
     CDS             650..>2375
                     /locus_tag="AC16HB_09380"
                     /inference="COORDINATES: protein motif:HMM:NF052715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5607039.1"
                     /translation="MTTPAAAGPLRFGRFEIQSNERRLLVDGRPAALGARAFDVLLAL
                     AERPGRLVSKRALMDLVWPGLVVQENNLAAQVSALRRVVGEEVIATIPGRGYRFVARI
                     ESTAAAHAAAPPPPLPPPALRTNLPVELPALLGRTYELDALGVLIDSHRLVTIVGAGG
                     IGKSLLTQHLLRARRGAYPQGVCWVELASVTDVAALPGAVATALDVDCGHGEPLAALI
                     GAVAPLALLLALDNAEHLLDAVAQLAKALHDAAPGVRMVVTSQAPLRLGAERVFRIGP
                     LAVPGATLPVAQALQFGAVALFVERAHAIDGRFALSDANAPAVIAICRSLDGLPLAIE
                     LAAARAPTLGVQPLLASMQDRLKLLTASRDRTAPERQQTLRAALEWSHGLLPPREQKV
                     FRRLGVMAGSASLGLIQQVIADDGEDVELDRWGVLDALDMLVDRSLVVVLSTDDDLEP
                     RYRLLESPRAYAIERLAESGERPDLQRRHAHAMADLFDAVYGEYFSGRVGVDDWLRRR
                     EADFDNAREALHWARAAGEVEVELRIGSVMLRALPPSLHAERIELADACEARIGPQVS
                     APLQLQAWI"
BASE COUNT          314 a          817 c          875 g          369 t
ORIGIN      
        1 agtcgatggt cttcatgaca ggtccgatgc tctggtgggg tggtcgatgc gagtgttcgg
       61 cggcgttcgt tgcggcgtca ctcgccgtcg tgctcggtgc ggggtgcacc gtgctcgcgc
      121 aggtcggcgg cgaccatcgc gctccagcgc tgaagttgtt ctggcgtggc cggccacgct
      181 gcgccgaaca gggacgggtc gtgtcgaacc atcatcagcg cgacgctcgc cgaggggacg
      241 tggcccgcgt cggcgagcag ggcaaagcgg ccataggcga tggcgtagcg atgcgcgagg
      301 aagtcgtcga tcgccgccgc atgccagagc gcgtgatgcg cgcgcacgga ttgcaggtaa
      361 gcggcctgga tcgggtcggc acgcaacggc gcggcgccga tcgtgagcag tgcgatcgcg
      421 agcgtcgtcg tgcaggcggc gcggacagtg gagcgggtgc gtttcatatc gtttcctttc
      481 ggtgcctgtg ggtcggggat acggcgtgcc gcagccaagg cggcactgtc cgcgcatccg
      541 ctcgccgggt cttgaagacg ctgtgaaaac ttctccaaag tgatgaatcg gcccgtcctg
      601 ccgctgataa gctggcgcgc tcggtcacca ctcgcgcctt gccctgccca tgacgacgcc
      661 ggcggccgca ggacccttgc gcttcggccg attcgaaatc cagtccaacg agcgccgtct
      721 gctcgtcgac ggccggcccg cggccctcgg cgcgcgcgcg ttcgacgtgc tgctggcgct
      781 ggccgagcgt cccggccgcc tcgtcagcaa gcgcgccttg atggacctcg tgtggccggg
      841 cctcgtggta caggagaaca acctcgccgc gcaggtcagt gcgctgcgca gggtcgtggg
      901 cgaagaggtg atcgcgacga ttccgggccg cggctatcgc ttcgtggcgc ggatcgagtc
      961 gaccgcggcg gcgcacgcgg cagcgccgcc tccgccgctc ccgccgccgg cgctgcgcac
     1021 caacctgccg gtcgagttgc ctgcactgct cgggcgcacg tacgaactcg atgcgctcgg
     1081 cgtgctgatc gacagccacc ggctcgtcac catcgttggc gcgggcggta tcggcaaatc
     1141 gctgctgacg cagcacctgc tgcgcgcacg gcgcggcgcg tatccgcagg gcgtgtgctg
     1201 ggtcgagctg gcgtcggtga ccgacgtcgc tgcgctgccc ggcgcggtcg ccaccgcact
     1261 ggacgtggac tgcggccacg gcgaaccact ggccgcgctg atcggggccg tggcaccgct
     1321 ggcactgctg cttgcgctcg acaacgccga gcacctgctc gacgccgtgg cgcagcttgc
     1381 caaggcactg cacgacgctg cgcccggcgt gcgcatggtg gtcaccagtc aggcgccgct
     1441 gagactcggt gcggagcgcg tcttccgcat cggtccgctc gcggtgcccg gcgcgacgct
     1501 gccggtggcg caggcgctgc agttcggcgc ggtggcgttg ttcgtcgagc gcgcgcacgc
     1561 gatcgacggg cgcttcgcgc tcagcgacgc caacgcaccc gcggtgatcg cgatctgccg
     1621 ttcgctcgac ggcctgccgc tggcgatcga actggccgca gcgcgcgcgc cgacgctcgg
     1681 cgtgcaaccg ctgctggcgt cgatgcagga tcggctgaag ctgctgaccg cgagccgcga
     1741 ccgcaccgcg ccggaacggc agcagacgct gcgcgccgcg ctggaatgga gccacggcct
     1801 gctgccgccg cgcgagcaga aggtgttccg gcgcctcggc gtaatggcgg gcagcgcgtc
     1861 gctcggcctg atccagcagg tgatcgccga cgacggcgag gacgtcgagc tggaccgctg
     1921 gggcgtgctc gacgcgctcg acatgctggt cgatcgctcg ctggtggtag tgctgtcgac
     1981 cgacgacgac ctcgaaccgc gttaccgcct gctcgaatca ccgcgcgcct atgcgatcga
     2041 gcgcctcgcc gagtcgggcg agcgcccgga cctgcagcgc cgtcacgcgc acgcgatggc
     2101 cgatctgttc gacgccgtgt atggcgagta cttcagcggc cgcgtcggcg tcgacgactg
     2161 gttgcgccgc cgcgaagccg acttcgacaa cgcacgcgag gcgctgcact gggcgcgcgc
     2221 cgcgggcgag gtcgaggtcg aactgcgtat cggctccgtg atgctgcggg cgctgccgcc
     2281 gtcactgcat gccgagcgca tcgaactggc cgacgcctgc gaggcgcgca tcggcccgca
     2341 ggtgtccgca ccgctgcagc tgcaggcgtg gatcg
//
LOCUS       DBNHBY010000377         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1849248, whole genome shotgun sequence.
ACCESSION   DBNHBY010000377 DBNHBY010000000
VERSION     DBNHBY010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1849248"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16HB_09385"
     CDS             complement(<1..452)
                     /locus_tag="AC16HB_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5607040.1"
                     /translation="MKVDPILLSVYARTFKSITDEMSISMEKTTRSPILCEAKDYVTG
                     LYDADGNMLEQTENLPILAFSLAPVCKYIKDYFGDEIYPGDVIFHNDVFSLGNQNNDV
                     AVYKPVFHGDKLVAWTAVKGHQADIGGNVRGGYNPNAVEVWQEALRIPA"
     gene            complement(449..>2017)
                     /locus_tag="AC16HB_09390"
     CDS             complement(449..>2017)
                     /locus_tag="AC16HB_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5607041.1"
                     /translation="ELPGAYLSVSTDVLPSIRFYERVSTTALNAYVGPKLNHYLDQLV
                     GRLHEIGLKGLLLIMQSNGGVISPQLARDKAALTLLSGPAGGPGAGLFYVRAHGQDKC
                     ITTDMGGTSFEASVAVRAPMVKNDGEIARHKIALPMLDIHTIGAGGGSIGWLDEGGLL
                     RMGPQSAGADPGPACYGKGGKLPTTTDANVVLGYLDPNYFAGGKMRLDAAAARRAIEE
                     HIATPMGLSVEDAAAGMYRVACNNMAQGVREVTIKRGFDPREFPFIPAGGAGPIHACL
                     ICQELEIPLQIVPREASVLCAFGMLMSELKHDFVRTFVSRLDGIDWQRLATLIDEMQR
                     DGERQLAEERIPAARRRHEVRLDCRYIKQYHEVSLPVPRELIDRRDTAGIAKAFHAEH
                     NRLFGYSLAQEATPIEIINVRVQAVGATDTPAYREEAWAGADSAHALKGRRNVYVPET
                     RRFGEVPVYDGHRLRCGNRIDGPALIEQETTAVFVSNSFDCVVDALGSFALFAKGRED
                     LVASCIRQTEEVAV"
BASE COUNT          311 a          690 c          670 g          346 t
ORIGIN      
        1 gccgggatgc gcagcgcctc ctgccacacc tcgaccgcgt tcgggttgta gccgccgcgc
       61 acgttgccgc cgatgtcggc ctgatggccc ttgaccgccg tccacgcgac cagcttgtcg
      121 ccgtggaaca ccggcttgta gacggcgacg tcgttgttct ggttgccgag cgagaacacg
      181 tcgttgtgga agatcacgtc gccgggatag atctcgtcgc cgaagtaatc cttgatgtat
      241 ttgcacacgg gcgcgaggga gaacgccagg atcggcaggt tctccgtctg ctccagcatg
      301 ttgccgtcgg cgtcgtacag tccggtcacg tagtccttcg cctcgcacag gatcggcgag
      361 cgcgtggtct tctccatcga gatgctcatc tcgtcggtga tcgacttgaa cgtgcgcgcg
      421 tacaccgaca gcaggatcgg atcgaccttc atacagcgac ctcctcggtc tggcggatgc
      481 aggatgcgac gagatcctcg cgccccttgg caaacaatgc gaacgagccg agcgcatcga
      541 ccacgcagtc gaaagaattg ctgacgaaga ccgccgtcgt ctcctgctcg atcagcgcgg
      601 ggccgtcgat gcggttgccg cagcgcaaac ggtggccgtc gtagaccggc acctcgccga
      661 accggcgcgt ctccggcacg tagacattac gccgtccctt cagcgcgtga gccgaatcgg
      721 cgccggccca cgcctcctcg cgatacgcgg gcgtgtcggt cgcgcccacc gcctgcacgc
      781 gcacgttgat gatctcgatc ggcgtggctt cctgtgccag cgaatagccg aacagccggt
      841 tgtgctcggc atgaaacgcc ttggcgatgc cggcggtgtc gcggcggtcg atcagctcgc
      901 gcggcacggg gagcgacacc tcgtggtact gcttgatgta gcggcagtcg aggcgcacct
      961 cgtggcggcg ccgcgcggca gggatgcgct cttcagcgag ctgccgctcc ccgtcgcgct
     1021 gcatctcgtc gatcagcgtt gcaagccgct gccagtcgat gccgtcgagc ctagagacga
     1081 acgtgcgcac gaagtcgtgc ttcagctcgc tcatcagcat gccgaacgcg cacagcaccg
     1141 aggcttcgcg cggcacgatt tgcagcggga tctccagctc ctggcagatc aggcaggcgt
     1201 ggatcggccc cgcgccgccc gccggaatga acgggaactc gcgcgggtcg aagccgcgct
     1261 tgatcgtcac ttcgcgcacg ccctgcgcca tgttgttgca ggcgacgcgg tacatgccgg
     1321 ccgcggcgtc ctcgactgac aatcccatcg gcgtggcgat gtgctcctcg atcgcgcgcc
     1381 gcgccgcggc ggcgtcgagc ctcatcttgc cgccggcgaa gtagttcgga tcgagatagc
     1441 cgagcacgac gttggcgtcg gttgtggtcg ggagcttgcc gcccttgccg tagcacgccg
     1501 gccccggatc ggcgccggcg ctctgcgggc ccatgcgcag caggccgcct tcgtcgagcc
     1561 agccgatcga gccgccaccg gcgccgatgg tgtggatgtc gagcatcggc agcgcgatct
     1621 tgtggcgcgc gatctcgccg tcgttcttga ccatcggcgc gcgcaccgcg accgacgcct
     1681 cgaagctggt gccgcccatg tccgtcgtga tgcacttgtc ctgaccgtgc gcgcgcacgt
     1741 agaacaggcc cgcgcccggc ccgccggcag gcccggacag cagcgtcagc gcggccttgt
     1801 cgcgcgccag ttgcggcgag atcacgccac cgttggactg catgatcagc agcaggccct
     1861 tgaggccgat ctcgtgcagc cgcccgacaa gctggtcgag gtagtgattc agcttcggcc
     1921 cgacgtaggc gttcagcgcg gtcgtgctga cgcgctcgta gaagcggatc gacggcagca
     1981 cgtcggtcga caccgacagg tacgcgcccg gcagctc
//
LOCUS       DBNHBY010000378         2205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_157548, whole genome shotgun sequence.
ACCESSION   DBNHBY010000378 DBNHBY010000000
VERSION     DBNHBY010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_157548"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16HB_09395"
     CDS             <1..288
                     /locus_tag="AC16HB_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5607042.1"
                     /translation="RANASALHHSNTSSRSCCGNCPSRWAAVTARFVYVRLHGHALTY
                     VSAYAPRTLARWAERIKTWRAESRDVYVFFDNTDAGSAHRDAGALARRCAG"
     gene            344..1213
                     /locus_tag="AC16HB_09400"
     CDS             344..1213
                     /locus_tag="AC16HB_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR5607043.1"
                     /translation="MATREVNRIVHSIRQREGGGFIVRRPFPTAGLDHLDPFLLLDEM
                     GPAEYGPGEAVGAPDHPHRGFETVTYMLDGEFVHEDSVGHRATIAAGDVQWMTAGDGV
                     IHSEMPADWIRQKGGRVHGFQLWVNLPARDKRIVPRYQDVRANEMPIWSAADGRVRVK
                     VIAGKVLGVQARAETRTPITYLDVLLDDGAEVALDVPAEQNAFVYVYGGAVTLGALAR
                     AARDGDMALLTNADSLALRATAPATRALVIAGVPLREPVARYGPFVMNSHEQIVEAIN
                     DYQSGRFGAIARG"
     gene            complement(1272..1538)
                     /locus_tag="AC16HB_09405"
     CDS             complement(1272..1538)
                     /locus_tag="AC16HB_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607044.1"
                     /translation="MQVKHLIGVAAVALAPLAAYADQGDKYLEQQVGTPKSTLTRAEV
                     RMQPTTPHLGQQHLVDLQTAATSHLTRADVLRELMAAAALPGGA"
     gene            complement(1725..2045)
                     /locus_tag="AC16HB_09410"
     CDS             complement(1725..2045)
                     /locus_tag="AC16HB_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR5607045.1"
                     /translation="MHDAQAYAVDYARIEPLRFSGDGAAALQRLLRIVAAMPGAQVIE
                     AKSDHLYVQFTTRWLRFTDDAEFWLSPSESVIHVRSASRIGRKDFGVNRARVEAIRAR
                     FAAA"
     gene            complement(1981..>2205)
                     /locus_tag="AC16HB_09415"
     CDS             complement(1981..>2205)
                     /locus_tag="AC16HB_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607046.1"
                     /translation="AEKGPPMTALKWIVVVVVIASMLLLAAGQLGLLKGRPPAAALAH
                     AKQRQQPGSSARCAGVRRRLRAHRAAALQR"
BASE COUNT          341 a          729 c          784 g          351 t
ORIGIN      
        1 cgcgcgaacg cgagcgcgct gcaccactcg aacacaagct cgcggtcgtg ctgtggcaac
       61 tgccccagtc gctgggccgc ggtcaccgct cgcttcgtct acgtgcggct gcacggccat
      121 gcgctcacct atgtgtccgc gtacgcgccg cgcacgctgg cgcgctgggc ggaacgcatc
      181 aagacctggc gcgccgaaag ccgcgacgtg tacgtgttct tcgacaacac cgacgccggc
      241 agcgcgcatc gcgacgccgg cgcactggca cgccgctgcg cgggttgaga tccggaggtt
      301 cggcctcacg tacgctcact tcggaaccgg acaagggaat gacatggcca cgcgtgaggt
      361 caatcgcatc gtccactcca tccgtcagcg cgaaggcggc ggcttcatcg tgcggcggcc
      421 gtttccgaca gcggggctcg accacctcga tcccttcctg ctgctcgacg agatgggccc
      481 ggccgagtat ggacccggcg aagcggtcgg tgcgcccgat cacccgcatc gcggcttcga
      541 gaccgtgacc tacatgctcg acggcgagtt cgtgcacgaa gacagcgtcg gccaccgcgc
      601 gacgatcgcg gccggcgatg tgcagtggat gaccgccggt gacggcgtca tccactccga
      661 aatgccggcc gactggatcc ggcagaaggg cgggcgcgtc catggcttcc agctgtgggt
      721 gaacctgccg gcgcgcgaca agcgcatcgt gccgcgctac caggacgtgc gcgccaacga
      781 gatgccgatc tggagcgccg ccgacggccg cgtgcgcgtc aaggtgatcg ccggcaaggt
      841 gctcggcgtg caggcgcgcg cggaaacgcg tacgccgatc acgtacctgg acgtgctgct
      901 ggacgatggc gccgaggtcg cgctcgacgt gccggccgag cagaatgcgt tcgtttacgt
      961 ctacggcggt gcggtgacgt tgggcgcgct tgcgcgcgcg gcccgggacg gagacatggc
     1021 gctgctgacc aatgccgatt cgctcgcgct gcgcgcgacc gcgccggcga cacgcgcgct
     1081 ggtgatcgcc ggcgtgccgc tgcgcgagcc ggtcgcccgt tacggcccgt tcgtgatgaa
     1141 ctcgcacgag cagatcgtcg aggcgatcaa cgattaccag tcgggccgct tcggcgcgat
     1201 cgcgcgcggg tgatggcgcg gcacaaacaa aaggggcgcc gcagggcgcc cctttccata
     1261 tttggtgcgg attacgcgcc gcccggcagg gccgccgcgg ccatcagttc gcgcaatacg
     1321 tcggcgcgcg tcaggtgcga cgtcgcggcg gtctgcaggt cgaccagatg ctgctggccc
     1381 aggtgcggcg tggtcggctg catccgcact tcggcgcggg tcagcgtgct cttgggcgtg
     1441 ccgacctgct gttcgaggta cttgtcgcct tgatccgcgt aggcggcaag gggggcgagg
     1501 gcgacggcgg cgacgccgat caggtgcttg acttgcatga cggactcctt gggttggttg
     1561 gatcaaacgg tgtgacgtgg ttgatacgtc cggtggacgg ctttggatgt catcgccgcg
     1621 ccacgtgccc atctgtcgag gaagctgcgc gcgtcttgca tcgttccgcg cagaaattca
     1681 tcggcgcggc ggcgtaagaa ggaaggggcg agtgcgacgc gccgtcaggc ggcggcgaag
     1741 cgcgcgcgga ttgcctcgac gcgcgcgcga ttgacgccga agtccttgcg tccgatgcgc
     1801 gatgccgagc ggacgtggat cacggactcc gacggcgaca gccagaactc ggcatcgtcg
     1861 gtgaacctga gccagcgcgt ggtgaactga acataaaggt ggtcggactt cgcttcgatc
     1921 acttgcgcgc ccggcatggc ggcgacgatc cgcagcagcc gctgtagcgc cgccgctcca
     1981 tcaccgctga agcgcagcgg ctcgatgcgc gcgtagtcga cggcgtacgc ctgcgcatcg
     2041 tgcagatgag cctggctgct gacgctgttt ggcgtgcgcg agggcggctg caggcggccg
     2101 gcccttcaac aggccgagct ggcccgcggc cagcagcagc atcgacgcga tcaccactac
     2161 gaccacgatc cacttcagcg ctgtcatcgg cggtcctttc tcagc
//
LOCUS       DBNHBY010000379         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1714557, whole genome shotgun sequence.
ACCESSION   DBNHBY010000379 DBNHBY010000000
VERSION     DBNHBY010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1714557"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1463
                     /locus_tag="AC16HB_09420"
     CDS             120..1463
                     /locus_tag="AC16HB_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607047.1"
                     /translation="MNSTAARMRRAVRARFALAPDDLLRDRTYRRLWLSILISSFGGQ
                     VSMLALPLTAAVLLHATPTQMGLLTAMEVLPFGLLSLPAGVWLDRVRKLPVYIAGESM
                     LALALTSVPVAWWLGWLAMPWLYVIAFVIGAVNTVSGSAAQIVLTQIVPRARLVEAHA
                     RNALANSAAEVAGPGAAGALIKLVGAPVAMLVDAVLITISTLILRGIRVTEAPQPVLA
                     RFWQARRDGLRFVAGHRLLVTMALLVGGWHMCHYAAQVVQILFATRTLGLSERAVGLC
                     YVGLGAGTVAASAIGYRISRRLGPAPTMVLGHAICGVGWLLGAAAPASAWGVAMFAAM
                     LLLFGVGAVLIFINFLAIRQAVTPTPLLGRMTSTMRWLIMVPAVPGALLGGWLGEFAG
                     LRSAMAVAGGAVLLFALLAWRQPLIRTMRELPQVDSDLTAVAAGGGTEPSPASPR"
     gene            1582..>2231
                     /locus_tag="AC16HB_09425"
     CDS             1582..>2231
                     /locus_tag="AC16HB_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5607048.1"
                     /translation="MASTGKPEPLRLHVPEPPGRPGRETDFSYLQVSPAGAVRRPPID
                     SKPFETQDVASALVRVLDDQGRVVGPWAPEVDPQLLRAALRAMMKTRVFDARMLIAQR
                     QKKLSFYMQSLGEEAVGIGQAFALEPDDMVFPTYRQQGILIARGYALFDMMCQLFSNA
                     RDPLKGRQMPVMYSSHRHSFYSIAGNLGIQYIQAVGWAMAAAIKGDTRIASAWIGDG"
BASE COUNT          294 a          743 c          813 g          381 t
ORIGIN      
        1 agcaattgct gagctcggcc gacgtctgca tgctcgaagc caaacgcctc ggccgcgacc
       61 gcatcgtcgt gcattcgatc cgggcgggcg agccggcttc ggttccgcac tgagcaccga
      121 tgaactcaac cgcggcgcgg atgcggcgcg cggtgcgtgc gcgctttgcg ctcgcgcccg
      181 acgacctgct gcgcgaccgc acctaccgca ggctgtggct gtcgatcctg atcagctcgt
      241 tcggcggcca ggtgtcgatg ctggcgctgc cgctcacggc ggcggtgttg ctgcatgcga
      301 ctccgacgca gatgggcctg ctgaccgcga tggaggtgct gccgttcggg ctgctgtcgc
      361 tgcccgccgg cgtatggctc gaccgggtgc gcaagctccc cgtctacatc gccggcgaat
      421 cgatgctggc gcttgcgttg acgagcgtgc cggtggcgtg gtggctcggc tggctcgcga
      481 tgccgtggct gtacgtgatc gcattcgtga tcggcgcggt gaacacggta tcgggcagcg
      541 cggcgcagat cgtgctcacg cagatcgtgc cgcgcgcgcg gctggtcgag gcgcatgcgc
      601 gcaatgcgct cgccaactcc gccgccgagg tcgccggccc cggcgcggcc ggcgcgctga
      661 tcaagctggt cggcgcgccg gtggcgatgc tggtcgatgc cgtgctgatc acaatctcga
      721 cgctgatcct gcgcggcata cgggtgaccg aggcgccgca gccggtgctt gcgcgcttct
      781 ggcaggcgcg gcgcgacggg ctgcgtttcg tggccggtca tcgactgctg gtgacgatgg
      841 cgctgctggt cggcggctgg cacatgtgtc attacgcggc gcaggtggtg cagatcctgt
      901 tcgccacgcg cacgctcggc ctgagcgagc gcgcggtcgg cctgtgctat gtcggcctcg
      961 gcgccggcac cgtggcggcg agcgcgatcg gctatcgcat ctcgcggcgg ctcgggccgg
     1021 cgccgacgat ggtgctcgga catgcgatct gtggcgtcgg ctggctgctc ggcgcggccg
     1081 cgcccgcgtc ggcgtggggc gtggctatgt tcgcggcgat gctgctgctg ttcggcgtcg
     1141 gcgcggtgct catcttcatc aacttcctcg cgatccggca ggcggtgacg cccacgccgc
     1201 tgctcggccg catgaccagc acgatgcgct ggctgatcat ggtgccggcc gtccccggcg
     1261 cgctgctcgg cggctggctc ggggagttcg cagggctgcg gtcggcgatg gcggtcgcgg
     1321 gtggcgccgt gctgctgttc gcactgctgg cgtggcgcca gccattgatc cgcacgatgc
     1381 gcgaactgcc gcaggtcgac agcgatctca ccgcggtcgc ggccggcggc gggaccgaac
     1441 catcgccggc cagcccgcgt tgagcgcctg cgcggaaaag ccgctacgct ttcgccgcgc
     1501 gactgcaggt cagccacaca atggtgaatc gctgcaggcg cagtcgactg tagcgagaca
     1561 cgatcgagcg cggagacaac gatggcatcg acaggaaagc ccgagccgct gaggctgcac
     1621 gtcccggaac cgccggggcg ccccggtcgg gaaaccgatt tctcctatct gcaggtgtcg
     1681 cccgccggcg cggtgcggcg accgcccatc gattcgaaac cgttcgagac gcaggacgtc
     1741 gccagcgcgc tggtgcgcgt gctcgacgat cagggccgcg tggttggccc gtgggcgccc
     1801 gaggtcgatc cgcaactgct gcgcgccgcg ttgcgcgcca tgatgaagac ccgcgtgttc
     1861 gacgcgcgca tgctgatcgc gcagcggcag aagaagctgt cgttctacat gcagagcctg
     1921 ggcgaggaag cggtcggtat cggtcaggcc ttcgcgctcg agcccgacga catggtcttt
     1981 ccgacctatc gccagcaggg catcctgatc gctcgcggct atgcgctgtt cgacatgatg
     2041 tgccagctgt tttcgaacgc gcgcgacccg ctcaagggcc ggcagatgcc ggtgatgtac
     2101 tcgtcgcacc ggcacagctt ctactcgatc gccggcaacc tcggcattca gtacatccag
     2161 gcggtcggct gggcaatggc cgcggcgatc aagggcgaca cgcgcatcgc atcggcctgg
     2221 atcggcgatg g
//
LOCUS       DBNHBY010000380         9012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_698956, whole genome shotgun sequence.
ACCESSION   DBNHBY010000380 DBNHBY010000000
VERSION     DBNHBY010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_698956"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..327
                     /locus_tag="AC16HB_09430"
     CDS             28..327
                     /locus_tag="AC16HB_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIR5607049.1"
                     /translation="MKPGIHPEYREVVFIDTSNDFKFITRSTVQTKEKITFEGKEYPV
                     YKLETSSESHPFYTGAQTRIVETGRVEKFRQKFAQKTEQVNAAAAQATAARARRK"
     gene            389..2125
                     /locus_tag="AC16HB_09435"
     CDS             389..2125
                     /locus_tag="AC16HB_09435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5607050.1"
                     /translation="MVFDTHPSPARVSAAAARPFPRVSLLALLAVFIGSGLVGRDPWY
                     QEDAAGFGVMWTMAHGTLADWLLPNVGGEYIGDGGPLAGWVGAVFIRLFGAWLGDVLA
                     SRLTVVLWFVVATGTVWYATYRLARRDEAQPVAFAFGGEAKPLDYGRMLADIAVLLFM
                     GTLGLVVRMHETTSLAASVALAGAVLFGLALALERAWAGALLVGAAIGLMALARGPLP
                     ALFIALGAFLAFMISLPRAQRLAAALVAPAIACAVFGLWPLATLLVPDTARAQFFAAW
                     IAEAKDSFGLAGPADLAWLLRTLAWYVWPLWPFALWALYAWRQGLARAHIAQPALVGA
                     ALLAAAFAAPQTEPPLLLLAAPLAVLAAFGAVSMRRAAESAIDWFAIVTVSFFGLVGW
                     AYFIAMLTGSPPKMAASIARLTPGYVPQIGVAAIACALAATAGWIALVVWRVVKHPEP
                     LWRGPLLAAGGLTALWFMLNVLFLPAINHARSYEPLAQRIRQEIDHASVAQACVFSFQ
                     LRPAHRAMLAYHGGIRFGGAHEDCPLALQRDLRRTHLDDAPPPGEWRMVWEGHWDARP
                     DETFRLFRRGRN"
     gene            2150..3517
                     /locus_tag="AC16HB_09440"
     CDS             2150..3517
                     /locus_tag="AC16HB_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5607051.1"
                     /translation="MSAPPVHSTSVRAIVDQAWPMFIAQLAIVASSGIDTAMAGRLSA
                     TDLAGVALGINVYVSVYIAFMGVVTAVGPIAAHHFGAGRLHAIGHDVGQALWLAAAFS
                     FVGILLLAWTDPWFALAQAPPAVSRITSDYLLAVAVGLPAALAARVFVALNSAVSRPR
                     ITMAINLVALALKIPLNALFMYGAGPLPALGGAGCGVATAVLSWLTVALSFAVWRLDP
                     YFERFRARRTLDFRAPQWSSQRELLVLGVPMGIAILFEVSSFTFMGILIARLGAETLG
                     AHQIVANIVSILFMVPLSIGFAGGALTAQSLGAGRARDARRIALRALRLTLLLAIALA
                     TLVWLARGPIVDLYTREPAVARIAATLLTLGTAFHVFDALQGVAFQTLRGYKVATAPM
                     LIYGVCLWGIGIGGGFWIAYSVTPFGPPFGASGFWSAAVVSLFGAATLLVTLLLAVSR
                     WRVRA"
     gene            complement(3567..4457)
                     /gene="htpX"
                     /locus_tag="AC16HB_09445"
     CDS             complement(3567..4457)
                     /gene="htpX"
                     /locus_tag="AC16HB_09445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HIR5607052.1"
                     /translation="MKRIALFVATNLAIMVVLMLFLNVISAVFGINFAGVGGRRLDIG
                     ALAVFALVVGFTGSFISLLMSKPMAKWSTGAQVIDQPRNAMEAWLVDVVRKHAQKAGI
                     GMPEVALYEGEPNAFATGASRNSALVAVSTGLLQSMSKDEVEAVLGHEIGHVANGDMV
                     TMTLIQGVMNTFVVFLSRVIGWVVDRVILKNDSDAPGMGYWVSVIVLDIALGFLAAII
                     VAWFSRQREFRADAASANLMGSPRPMINALARLGGMEPGALPQQVKAMGINSAPSGML
                     ALFSTHPPIEQRIAALQARG"
     gene            4739..6265
                     /locus_tag="AC16HB_09450"
     CDS             4739..6265
                     /locus_tag="AC16HB_09450"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR5607053.1"
                     /translation="MTTSIKQADLIESVAGALQYISYYHPVDFIRSLARAYEVEQSAA
                     AKDAIAQILSNSRMCAEGKRPICQDTGIVNVFLKVGMDVRWDGFTGTIEDAVNEGVRR
                     GYLNPDNKLRASVVESPEFGRRNTTDNTPAVTHLQLVPGKTVEVTVAAKGGGSENKSK
                     FVMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLMAKEALMGDIDMTE
                     LLQRGPKTDEEKLRVELYEKVNALGIGAQGLGGLTTVLDVKIKMYPTHAASKPVAMIP
                     NCAATRHAHFVLDGSGPAYLDPPSLDLWPDVNWQPDYGKSKRVNLDTLTKAEVASWKP
                     GDTLLLNGKMLTGRDAAHMRIQDMLAKGEKLPVDFTNRVIYYVGPVDPVRDEVVGPAG
                     PTTATRMDKFTEMMLAQTGLIAMIGKAERGPVAIEAIRKHKSAYLMAVGGAAYLVAKA
                     IRTARTLAFADLGLEAIYEFEVKDMPVTVAVDANGTSVHQTGPREWQAKIGKIPVAVG
                     "
     gene            6358..7479
                     /locus_tag="AC16HB_09455"
     CDS             6358..7479
                     /locus_tag="AC16HB_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5607054.1"
                     /translation="MKARRAWCDEAVHRIEADFIRSADTHLLRVTIPGAPDIPLYFKD
                     ESVHPTGSLKHRLARSLFLYGLCNGHIGPTTTIVEASSGSTAVSEAYFARLIGLSFVA
                     VMPRSTSPEKIALIEYQGGRCHLVDDPGTVYAEAARLAAAGDGHYMDQFTYAERATDW
                     RGNNNIAESIFSQMAAEPFTVPTWIVVGAGTGGTSSTLGRFVRYRRHATQICVADPEG
                     SVFHDYWASGDMSLTSRQSSRIEGIGRPRVEPSFNRDVVDRSIRVPDALAMAALRFLE
                     DKLGRRVGGSTGTQFVAAAALAAEMKRARRRGAVVTLLCDGGERYRSTYYNDAWLAQN
                     GLDIAPGLAALERFWRRGEWNAMPETRRSTDSGRRDRRR"
     gene            complement(7424..8329)
                     /gene="cysM"
                     /locus_tag="AC16HB_09460"
     CDS             complement(7424..8329)
                     /gene="cysM"
                     /locus_tag="AC16HB_09460"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HIR5607055.1"
                     /translation="MNYASIEATIGNTPLVRLQRLSGVDGAARGNVILGKLEGNNPGG
                     SVKDRPAYSMIRRAEERGEIRPGDRLIEATSGNTGIALAMIAAMKGYRMTLIMPDDLS
                     AERRASMTAYGAELILTPHAKGGMEYARDLAMQMQVHGEGKVLDQFANADNPRAHFET
                     TGPEIWRDTQGRITHFVSSMGTTGTIMGVSRYLKERNSAVHIIGAQPAEGSRIPGIRK
                     WPDAYLPKIYDAARVDRLEYVSQAEAEAMARRLAAEEGIFAGVSSGGACAIALRIAAE
                     VRDATIVFIVCDRGDRYLSTGVFPA"
     gene            complement(8326..8595)
                     /locus_tag="AC16HB_09465"
     CDS             complement(8326..8595)
                     /locus_tag="AC16HB_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR5607056.1"
                     /translation="MPAWLLVLLAPSVAGALEINEASRAQLEQLNGLGVARVEQILRE
                     RERAPFVDWDDLRARVKGIGVRGAQRLGAQGLTVNGRAPREQQKP"
     gene            complement(8601..>9012)
                     /locus_tag="AC16HB_09470"
     CDS             complement(8601..>9012)
                     /locus_tag="AC16HB_09470"
                     /inference="COORDINATES: protein motif:HMM:NF037181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR5607057.1"
                     /translation="LVARRLTDALDKVGRRGEALNLLRRGLMDAPSIDLLDAAVERVA
                     EWESRAAAEELLREELKRHPSLLGYERLLAMHGVADEALAGLHPLIQAQTRKLARYRC
                     GKCGFRAREFHWQCPGCTNWDSYPPRRIEELDAA"
BASE COUNT         1379 a         3034 c         3081 g         1518 t
ORIGIN      
        1 gactggcggc cgcaacgaaa gcaacccatg aagcccggca tccaccccga ataccgcgaa
       61 gtcgtgttca tcgacacgtc gaacgacttc aagttcatca cccgctcgac cgtgcagacg
      121 aaggagaaga tcaccttcga aggcaaggag tacccggtct acaagctcga gacctcgtcc
      181 gagtcgcacc cgttctacac cggcgcgcag acgcgcatcg tcgagacggg ccgggtcgag
      241 aagttccggc agaaattcgc gcagaagacc gagcaggtca atgccgccgc cgcccaggca
      301 accgctgcca gggcgcgccg gaaatagacc ctccgggcac gcgccgacat gtgacaaagg
      361 cagccgcgcg ctgccttttt tttcagccat ggtcttcgac acccacccgt cgcccgcccg
      421 cgtcagcgcg gccgcggcgc ggccgttccc gcgcgtgtcg ctgcttgcgc tgctggccgt
      481 gttcatcggc agcggcctcg tcggccgcga tccgtggtac caggaggatg cggcgggatt
      541 cggcgtcatg tggacgatgg cgcacgggac gctggcggac tggttgctgc cgaacgttgg
      601 cggcgagtac atcggcgacg gcggcccgct ggcaggctgg gtgggcgcgg tcttcatccg
      661 gctcttcggc gcctggctgg gcgacgtgct ggcctcacgt ctgactgtcg tgctgtggtt
      721 cgtggtcgcg accgggacgg tgtggtacgc aacctatcgg ctggcgcggc gcgacgaggc
      781 gcaaccggtt gcgttcgcgt ttggcggcga ggccaagccg cttgattacg gccgcatgct
      841 ggccgacatc gcggtgctgc tgttcatggg cacgctcggc ctcgtggtgc gcatgcacga
      901 gacgaccagt ctggctgcat cggtcgcgct cgccggcgca gtgctgttcg ggctcgcgct
      961 tgcgcttgag cgggcatggg cgggcgcgct gctggtcggc gcggcgattg gactgatggc
     1021 gctggcgcgc ggtccgctgc cggcgttgtt catcgcgctc ggcgcgttcc tcgcgttcat
     1081 gatttcgctg ccgcgcgcgc agcgcctcgc agccgcgctg gtcgcgcccg cgatcgcatg
     1141 cgcggtcttc ggcttgtggc cgctggcgac gctgctcgtc cccgatacag cgcgcgccca
     1201 gttcttcgcc gcctggattg cggaggcaaa ggattctttc gggctcgccg gcccggccga
     1261 cctcgcgtgg ctcctgcgca cgctggcctg gtacgtgtgg ccgctgtggc cgttcgcgct
     1321 gtgggcgctg tacgcgtggc gacagggcct ggcgcgcgca cacatcgccc agcctgcact
     1381 cgtcggcgca gcgctgctcg ccgcggcgtt cgcggcaccg cagaccgagc cgccgctgct
     1441 gctgctggcg gcgccgttgg ccgtgcttgc ggcattcggc gccgtcagca tgcgccgggc
     1501 ggcggaaagc gcgatcgact ggttcgcaat cgtgacggtg tcgttcttcg gactcgtcgg
     1561 ctgggcgtat ttcatcgcga tgctgaccgg cagcccgccc aagatggctg cttcgatcgc
     1621 gcgcctgact ccgggctacg tgccgcagat cggggttgcc gcgatcgcat gcgcgctggc
     1681 cgcgaccgcg ggctggatcg cgctcgtcgt atggcgcgtc gtgaagcatc ccgagccgct
     1741 ctggcgcggc cctttgctcg cggctggagg gctgacggcg ctgtggttca tgttgaacgt
     1801 gctgttcctg ccggcgatca accatgcgcg ctcgtacgag ccgctcgcgc agcggatccg
     1861 tcaggagatc gaccacgcca gcgtggcgca ggcgtgcgtg ttctcgttcc agctgcggcc
     1921 ggcacaccgc gccatgctcg cgtatcacgg cggcatccgc ttcggcggcg cgcacgagga
     1981 ctgcccgctg gcgctgcagc gtgacctgcg acgaacacat ctcgacgacg cgccgccgcc
     2041 cggcgaatgg cgcatggtct gggaaggcca ctgggacgcg cggcccgacg agacgtttcg
     2101 gctgttccgc cgcggacgga actagccgcg cgcgcggagc gcaccgccga tgtcagcgcc
     2161 accggtccat tcgacctccg tgcgcgcgat cgtcgaccag gcgtggccga tgttcatcgc
     2221 gcaactggcg atcgtcgcca gttccggcat cgacaccgcg atggccggcc ggctgtcggc
     2281 gaccgatctg gcaggcgtgg cgctcggcat caacgtgtac gtgtcggtgt acatcgcgtt
     2341 catgggcgtg gtcaccgcgg tcgggccgat tgccgcccac catttcggcg ccggccgcct
     2401 gcatgcgatc ggacacgatg tcgggcaggc gctgtggctg gccgcggcgt tctcgttcgt
     2461 cggcatcttg ctgctggcct ggaccgaccc ctggttcgcg ctcgcgcagg cgccgcccgc
     2521 ggtctcccgc atcacctccg actacctgct cgcggtggcg gtcgggctgc ccgccgccct
     2581 cgccgcgcgc gtcttcgtcg cgctgaattc agcggtgtcc cggccgcgga tcacgatggc
     2641 gatcaacctc gtcgcgctgg cgctgaagat cccgctgaac gcgctgttca tgtacggcgc
     2701 cggaccactg cccgcgctcg gcggcgccgg ctgcggcgtg gcgacggcgg tgttgtcgtg
     2761 gctcaccgtc gcgctgagct tcgcggtgtg gcgcctcgat ccgtacttcg agcgctttcg
     2821 cgcgcggcgc accctcgact tccgcgcgcc gcagtggtcg agccagcgcg agttgctggt
     2881 gctcggagtc ccgatgggca tcgcaatcct gttcgaggtc agctcattca ccttcatggg
     2941 aatcctgatc gcgcgcctcg gcgcagagac gctcggcgcg caccagatcg tcgcgaacat
     3001 cgtgtcgatc ctgttcatgg tgccgctgtc gattgggttc gccggcggcg cgctgaccgc
     3061 gcaatcgctc ggcgcaggcc gcgcgcgcga tgcgcggcgc attgcgctac gcgcgttgcg
     3121 gctgacgctc ctactcgcga tcgcgctggc gaccctcgtg tggctcgcgc gcggtccgat
     3181 cgtcgatctc tacacgcgcg agcctgcggt ggcgcggatc gctgcgacgc tgctcacctt
     3241 gggcaccgcg ttccacgtgt tcgacgccct gcaaggcgtg gccttccaga cgctgcgtgg
     3301 ctacaaggtc gcgaccgcgc cgatgctgat ctacggcgtg tgtctgtggg gcatcggcat
     3361 cggcggcggg ttctggatcg cgtattcggt cacgccgttc ggaccgccgt tcggcgcgtc
     3421 cggattctgg tcggcggcag tggtgagcct gttcggcgcg gcgacgctct tggtgacctt
     3481 gctgcttgcg gtgtcgcgct ggcgcgtgcg cgcatgaaaa gaagagggcc gggaatgccc
     3541 ggcccttctt gcagcttgcg atgcgctcag ccgcgcgcct gcagcgcggc gatgcgctgc
     3601 tcgatcggcg gatgggtcga gaacagcgcc agcatgccgc tcggcgcgct gttgatgccc
     3661 atcgccttga cctgctgcgg cagtgcgccc ggctccatgc cgcccaggcg cgccagcgcg
     3721 ttgatcatgg gccgcgggct gcccatcagg ttggccgagg ccgcgtcggc gcggaactcg
     3781 cgctggcgcg aaaaccaggc gacgatgatc gcggcgagaa agcccagtgc gatgtcgagc
     3841 acgatcaccg acacccagta acccatcccg ggcgcgtcgc tgtcgttctt caggatcacg
     3901 cggtcgacga cccagccgat cacgcgcgac aggaacacga cgaacgtgtt catcacgccc
     3961 tggatcagcg tcatcgtcac catgtcaccg ttggcgacgt gcccgatctc gtgaccgagc
     4021 acggcctcga cctcgtcctt gctcatcgac tgcagcaggc ccgtcgacac cgcgaccagc
     4081 gcggagttgc gactggcacc ggtggcaaag gcattcggtt cgccttcgta cagcgccacc
     4141 tcgggcatgc cgatgccggc cttttgcgcg tgcttgcgca cgacatccac cagccaggcc
     4201 tccatcgcat tgcgcggctg gtcgatcacc tgcgcaccgg tcgaccactt ggccatcggc
     4261 ttggacatca gcagcgagat gaacgagccg gtgaagccga ccaccagagc aaagaccgcg
     4321 agcgcgccga tgtcgagccg gcgtccgccg accccggcga aattgatgcc gaagaccgcg
     4381 ctgatgacat tcaggaacag catcagcacg accatgatcg caaggttcgt agccacgaac
     4441 agcgcgatgc gtttcatacg ttctccacgg aaagttgagg ggaaagcggc gccgagtcta
     4501 tccaagaccg ggcggacgga ccaatcagag ttttccgggt ctacatgact cctcgtcgcg
     4561 ataccagttg gaatcggccc tttggaccgg aaccccgata cggggttcaa catgggggca
     4621 atgcgacctg catcaagagg ctttgccccg ggttcggaca tgctgcgcca gtaagatttc
     4681 agggttttcc cgtgtgcgcg ccgcccgcac gctgccatcc gaacatcgag aggaatccat
     4741 gacgacgtcc atcaagcagg ccgacctgat cgaaagcgtg gcgggcgcgc tgcaatacat
     4801 cagctactac cacccggtcg atttcatccg cagcctcgcg cgtgcatatg aagtcgaaca
     4861 gagcgcagcc gccaaggacg cgatcgcgca gatcctgagc aactcgcgca tgtgcgccga
     4921 aggcaagcgg ccgatctgcc aggacacagg catcgtcaac gtgttcctga aggtcggcat
     4981 ggacgtgcgc tgggacggtt tcaccggaac gatcgaggac gcagtcaatg aaggcgtgcg
     5041 gcgcggttat ctgaatcccg acaacaagct gcgcgcgtcg gtcgtcgagt cgccggagtt
     5101 cggccgcagg aacaccacgg acaacacgcc cgccgtgact cacctgcagc tcgtccccgg
     5161 caagacggtc gaggtgacgg tcgccgcgaa gggcggcggc agcgagaaca agagcaagtt
     5221 cgtgatgctc aacccgagcg actcgatcgt cgactgggtg ctgaagacgg tgccgacgat
     5281 gggtgccggc tggtgcccgc cggggatgct cggcatcggc atcggcggca ctgcggagaa
     5341 ggcgatgctg atggccaagg aagcgctgat gggcgacatc gacatgaccg agcttctgca
     5401 gcgaggaccg aagacggatg aagagaagct gcgcgtcgaa ctgtacgaga aagtcaatgc
     5461 gctcggcatc ggcgcgcagg ggctcggagg actgacgacc gtcctcgacg tgaagatcaa
     5521 gatgtatccc acgcacgcgg cgagcaagcc ggtggcgatg attccgaatt gcgcggccac
     5581 gcgccacgcg catttcgtgc tcgacggcag cggtcccgcg tacctcgatc cgccgtcgct
     5641 cgatctgtgg ccggacgtga actggcagcc ggactacggc aagagcaagc gggtgaacct
     5701 cgacacgctg acgaaggccg aagtcgcctc gtggaagccc ggcgacaccc tgctgctgaa
     5761 cgggaagatg ctcacgggcc gcgatgccgc gcatatgcgc atccaggaca tgctcgcgaa
     5821 gggcgagaag ctgccggtcg acttcaccaa ccgcgtgatc tactacgtcg gccccgtcga
     5881 tccggtgcgg gacgaggtcg tcggccccgc gggcccaacg accgcgacgc gcatggacaa
     5941 gttcactgaa atgatgctgg cgcagaccgg cttgatcgca atgatcggca aggccgagcg
     6001 cgggccggtc gcgatcgagg cgatcaggaa gcacaagagc gcgtacctga tggcagtcgg
     6061 tggcgcggca tatctggtcg ccaaggcgat caggaccgca cggacgcttg cattcgccga
     6121 cctcggcctg gaagcgatct acgagttcga ggtcaaggac atgccggtca cggttgcagt
     6181 cgacgcgaac gggacgtcgg tgcatcagac cgggccgcgc gagtggcagg cgaagatcgg
     6241 caagattccg gtagcggtgg ggtgagcgtg gttgatgctt gttcgctgtt cggcgttcgt
     6301 tgaaggcgcc gggtgattga aggacgacga gcgacgagcc gtgaagtgtc agcaggcatg
     6361 aaggctcgcc gcgcgtggtg cgacgaggca gtccaccgca tcgaggccga cttcattcgc
     6421 tccgccgaca cgcatctgct gcgcgtcacg attcccggcg cgcccgatat tccgctgtac
     6481 ttcaaggacg agtcggtaca cccgacggga agcctaaagc accggctggc gcgctcgttg
     6541 tttctctacg ggctgtgcaa cggccacatc ggaccgacga cgacgatcgt ggaagcgtcg
     6601 tccggatcga ccgccgtgtc ggaggcgtac ttcgcgcgct tgatcggcct gtcgttcgtt
     6661 gcggtgatgc cccgctcaac gtcgccggaa aagatcgcgc tgatcgagta tcagggcggc
     6721 cgctgtcatc tggtcgacga tcccggcacc gtctacgcgg aggcggcgcg gctcgctgcc
     6781 gcgggcgacg gccactacat ggaccagttc acgtacgccg agcgcgcgac cgactggcgc
     6841 ggcaacaaca acatcgcgga atcgatcttc tcgcagatgg cggccgagcc gttcacggtg
     6901 ccgacgtgga tcgtggtcgg cgcgggcacc ggcggtacca gcagcacgct cggacgcttc
     6961 gtgcgctacc gccgccatgc gacgcagatc tgcgtggccg atccggaagg ctcggtgttc
     7021 cacgactact gggcgagcgg cgacatgtcg ctgacttcgc ggcagtcgtc gcgcatcgag
     7081 ggcatcggcc gcccacgggt cgagcccagc ttcaaccgcg acgtggtcga ccgttcgatc
     7141 cgcgtgccgg acgcgctcgc gatggcagcg ctgcgctttc tcgaagacaa gctcggtcgc
     7201 agggtcggcg gttccaccgg cacgcagttc gtcgccgcgg cggcgcttgc cgccgagatg
     7261 aagcgggcgc gccggcgcgg cgcggtcgtc actctgctgt gcgacggtgg cgagcgctat
     7321 cgcagcacgt actacaacga cgcctggctc gcacagaacg gactcgacat cgcgccggga
     7381 ctcgcggcgc tggagcgctt ctggaggcgc ggcgagtgga atgctatgcc ggaaacacgc
     7441 cggtcgacag atagcggtcg ccgcgatcgc agacgatgaa gacgatggtc gcgtcgcgca
     7501 cctcggccgc gatgcgcagc gcgatcgcgc acgcgccgcc ggaggagacg ccagcgaaga
     7561 tgccctcctc ggctgccagg cgccgcgcca tcgcttcagc ctcggcctgc gacacgtatt
     7621 cgaggcgatc gacgcgcgca gcgtcataga tcttcggcag atacgcatcc ggccatttgc
     7681 ggatgcctgg gatgcgcgag ccctcggccg gctgcgcgcc gatgatgtgg actgcggaat
     7741 tcctctcctt caggtagcgg ctcacgccca tgatcgttcc ggtcgtgccc atgctgctga
     7801 cgaaatgcgt gatccgcccc tgcgtgtcgc gccagatctc ggggccggtc gtctcgaagt
     7861 gcgcgcgcgg gttgtcggca ttggcgaact ggtcgagcac cttgccctcc ccgtgcacct
     7921 gcatctgcat tgcgaggtcg cgcgcgtact ccattccgcc cttcgcgtgc ggcgtcagga
     7981 tcagttcggc gccgtatgcg gtcatgctgg cgcgccgctc ggcggacagg tcgtccggca
     8041 tgatcagcgt catgcggtag cccttcatcg cggcgatcat cgccaatgcg atccccgtgt
     8101 tgccgctggt ggcctcgatc agccggtcgc cgggccggat ctcgccgcgt tcctcggcgc
     8161 ggcggatcat cgagtacgcg ggccggtcct tgacggagcc gccgggattg ttgccctcga
     8221 gcttgccgag gatcacgttg ccgcgcgctg cgccgtccac gccagacagg cgctgcagcc
     8281 gcaccagcgg tgtattgccg atcgtcgctt cgatgctcgc gtagttcatg gcttctgctg
     8341 ctcccgcggc gcgcggccgt tcacggtgag gccttgggcg ccgagccgct gcgcgccccg
     8401 gacaccgatg cctttgaccc gtgcgcgcag gtcatcccaa tccacgaacg gcgcccgttc
     8461 gcgctcccgc agaatctgct cgacgcgcgc cacgcccaga ccattcaact gctcgagctg
     8521 cgcacggctc gcttcgttga tttcgagcgc cccggccaca ctcggcgcga gcagtaccag
     8581 gagccacgcg ggcaagcgcc tcatgccgcg tccagttcct cgatccggcg cggcgggtaa
     8641 ctgtcccagt tcgtgcagcc cgggcactgc cagtggaact cgcgcgcgcg aaagccgcac
     8701 ttgccgcagc gatagcgcgc gagcttgcgc gtctgcgcct ggatcaaggg gtgcaggccg
     8761 gccagcgcct cgtcggcgac gccgtgcatg gcgagcaggc gctcgtagcc gagcagcgaa
     8821 ggatgccgct tcagttcctc gcgcagcagc tcctcggcag ccgcgcggct ttcccattcc
     8881 gccacgcgct ccaccgcggc gtcgagcagg tcgatcgacg gcgcatccat caacccgcgc
     8941 cgcagcaggt tcagtgcttc gccgcggcgg ccgaccttgt cgagcgcgtc ggtcaggcgg
     9001 cgcgcgacca gc
//
LOCUS       DBNHBY010000381         5290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_902192, whole genome shotgun sequence.
ACCESSION   DBNHBY010000381 DBNHBY010000000
VERSION     DBNHBY010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_902192"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16HB_09475"
     CDS             complement(<1..746)
                     /locus_tag="AC16HB_09475"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5607058.1"
                     /translation="MKLFALNATRELGGRVAAALGRALDAHEEREFEDGEHKARPLID
                     VRGADAYVIQSTHGDAQASANDKLMRLLLFAACLRDHGAARVTAVVPYLAYGRKDRQT
                     KPRDPVATRYVAQLIEAAGIGAMVALEVHNVAAFQNAFRIPTRHLESAPLFLPRAQAL
                     ARAGPLAAVSPDPGGVKRAQLFREALEAQLGQPVGSAYLEKRRSAGVVSGSLLAGDVR
                     GATVLLVDDLISTGGTMVRAAETCLAAGATR"
     gene            861..2219
                     /locus_tag="AC16HB_09480"
     CDS             861..2219
                     /locus_tag="AC16HB_09480"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR5607059.1"
                     /translation="MTAPNSGSALLTDLYELTMLQAYRACGMNDTAVFELFVRKLPPQ
                     RGFLLAAGLAHVVEYLRELAFEREEIAWLATTRLFSDDFLASLESLRFTGDLHAVPEG
                     TVVFADEPLARITAPMREAQLVESRVMNLLHYATTVATKAARCVLAAGGKPLIDFGLR
                     RAHGAEAGLLSARASYIAGFAGTATALAGSRYGIPVFGTMAHSFVQAHDDEAQAFLAF
                     ARAQPSNATLLIDTYDTEAAARKVVEIARVLGAEGIAIRGVRIDSGDLAEHARRVRRI
                     LDAGGLARAQILASGNLDEYRLRELVADVAPIDAFGVGTRMNTSADAPFLDCAYKLAE
                     YAGVGRRKRSEGKASWPGRKQVFRALDAQGRFARDVLSLQNDAQPGTPLLAPVLRAGE
                     LVAPLPALADARSHAQSQLAALPTALRALDTPAPHYEVIVAPALRALAEEVDRRTRRE
                     AG"
     gene            2352..2606
                     /locus_tag="AC16HB_09485"
     CDS             2352..2606
                     /locus_tag="AC16HB_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607060.1"
                     /translation="MDVETSRIVQHPDGYFWMSEDGKRETGPFASFDDALADMLATAE
                     SESEPGESLQEAEDEIGIADWIDPDTREPAEERRPRIEDQ"
     gene            complement(2617..3084)
                     /locus_tag="AC16HB_09490"
     CDS             complement(2617..3084)
                     /locus_tag="AC16HB_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5607061.1"
                     /translation="MRIGEICTREVVYCERATSVVELAQLMRRHHVGDVIVADAVGAK
                     LIPVGIVTDRDLVIDVLAQRVDPAEVTARDLMRKGLALARETEAVYDAIVRMRDSGIR
                     RLPVIDEHDCLIGVLTADDVTEFLAEELTALAAVGPRQVAHEQAARALPDIAV"
     gene            3192..4103
                     /locus_tag="AC16HB_09495"
     CDS             3192..4103
                     /locus_tag="AC16HB_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5607062.1"
                     /translation="MQLQGPGPRAAQVMRQPGAFALRVLTGFRANQGLLLAGAVAYYA
                     LLSIVPLLILTVIALSRVIDPAELLQTLGRYLEWLIPGQSQIIVAELGNFLAHRDVLG
                     WVLLATMIFFSSLAFTVLENAMCVIFHHRVAIRRRRFLVSALIPYCYILSLAVGLLLV
                     TLVAGSLQAMGQESVEFLGRTWSLGGVSGALLYLLGVAGEIFVLTSVYMVMPVGRLRL
                     THALIGAVTATLLWELTRHVLVWYFATLSHIGTVYGSLTTAIVVLLSLELGSTLLLFG
                     AQVIAEFERIGTAASEAPSEPMRLDAG"
     gene            4168..4695
                     /locus_tag="AC16HB_09500"
     CDS             4168..4695
                     /locus_tag="AC16HB_09500"
                     /inference="COORDINATES: protein motif:HMM:NF022161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HIR5607063.1"
                     /translation="MNFSLDQDFPADPARLWAALGRRDYVEQKYRSLGAATLRILRFE
                     VTRDSIDVELERSAPVARKALPAWARPFVADRQVMRHHTRWRRADVTRIDAELDIWPV
                     GLPVHAHGVGTLTGRPPGRTNMTLHFEVKCRLPAVGATAARLFADQVKEALQADHAFT
                     LDYLAADASPLQRPR"
     gene            4781..>5290
                     /locus_tag="AC16HB_09505"
     CDS             4781..>5290
                     /locus_tag="AC16HB_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5607064.1"
                     /translation="MNRLPSESLGEELRRAAREAPERPFIRMRTGEWSYGALDEESDR
                     LATGLQRLGVAHGDHVSLLAPNCIEFVALWFALAKLGAVAAPVNTAFRGAALADAIEI
                     VRSRLLVLHADHVQQFAEVSPRLEHIEQVVVIGEQISLERPVLTYARLRDAVALAPPV
                     PVRASDLCLL"
BASE COUNT          784 a         1828 c         1826 g          852 t
ORIGIN      
        1 cgcgtcgcgc cggccgcaag gcacgtctcc gccgcgcgca ccatcgtgcc gccggtgctg
       61 atcaggtcgt cgaccagcag cacggtggct ccacgcacgt cgcccgcgag cagcgagccc
      121 gacaccacgc cggcgctgcg ccgcttctcg agatacgcgc tgccgacggg ctggccgagc
      181 tgtgcctcca gcgcttcgcg gaacagctgc gcgcgcttga cgccgccggg gtcgggcgac
      241 acggccgcga gcggcccggc gcgcgccagc gcctgcgcgc gcggcaggaa gagcggcgcc
      301 gattcgaggt gccgcgtcgg aatgcggaac gcgttctgga acgcggccac gttatgcacc
      361 tcgagcgcga ccatcgcacc gatgccggcc gcctcgatca gttgcgcgac atagcgcgtc
      421 gccaccgggt cgcgcggctt ggtctgccgg tccttgcgcc cataggcgag gtacggcacc
      481 accgccgtca cacgcgccgc accgtggtcg cgcaggcagg cggcgaacag cagcaaccgc
      541 atcagcttgt cgttggcgct tgcctgcgca tcgccgtgcg tgctctggat cacgtacgca
      601 tcggcgccgc gcacgtcgat gagcgggcgc gccttgtgct cgccgtcctc gaactcgcgc
      661 tcctcgtggg catcgagcgc gcgcccgagt gccgcggcga ccctgccgcc gagctcgcgc
      721 gtcgcgttca acgcgaacag cttcatcggt tccatacgcg tcgacgctac cgccgcctgc
      781 ggcgcggccg ttgagggggg tcaacgcgag taatctggtg ttggctagct tgatcttcgg
      841 atggcccgcg aaagaccgcc atgacggcgc cgaattccgg cagtgcgctg ctgaccgacc
      901 tgtacgagct gacgatgctg caggcctacc gcgcctgcgg catgaacgac acggcggtat
      961 tcgagctgtt cgtgcgcaag ctgccgccgc agcgcggttt tctgctcgcg gcaggactcg
     1021 cgcacgtggt cgagtacctg cgcgaactcg ccttcgaacg cgaggagatc gcgtggctgg
     1081 cgaccacacg gctcttcagc gatgatttcc tcgcgtcgct cgagagcctg cgcttcaccg
     1141 gcgacctgca cgcggtgccg gagggcacgg tcgtgttcgc cgacgagccg ctcgcccgca
     1201 tcaccgcgcc gatgcgcgag gcgcagctcg tcgaaagccg ggtgatgaat ctgctgcatt
     1261 acgcgaccac tgtcgcgacc aaggccgccc gctgcgtgct cgccgcgggc ggcaagccgc
     1321 tgatcgactt cggcctgcgg cgcgcgcacg gtgccgaggc cggcttgctg tcggcgcgcg
     1381 ccagctacat cgccggcttc gccggcaccg cgaccgcact tgcggggagc cgctacggca
     1441 tcccggtgtt cggcaccatg gcgcactcgt tcgtgcaggc gcacgacgac gaggcccagg
     1501 cgttcctcgc cttcgcacgc gcgcagccgt ccaacgcgac gttgctgatc gacacctacg
     1561 acaccgaggc ggccgcgcgc aaggtggtcg agatcgcgcg cgtgctcggc gccgaaggca
     1621 ttgcaatccg cggcgtgcgc atcgacagtg gcgatctcgc cgagcacgcg cggcgcgtgc
     1681 gccgcatcct cgacgccggc gggctcgcgc gcgcgcagat ccttgccagc gggaacctcg
     1741 acgagtaccg gctgcgcgag ctcgtggcag atgtcgcgcc gatcgacgca ttcggcgtcg
     1801 gcacgcgcat gaacacgtcg gccgacgcgc cgtttctcga ctgcgcgtac aaactcgccg
     1861 aatacgcggg tgtgggccgg cgcaagcgct ccgaaggcaa ggcgagctgg ccggggcgca
     1921 agcaggtgtt ccgcgcgctc gacgcgcagg gccgctttgc acgcgacgtg ctgagtctgc
     1981 aaaacgatgc gcaacccggc acaccgctgc tggcgcccgt gctgcgtgcg ggcgaactcg
     2041 tcgcgccgct gccggcgctc gcggacgcgc gcagtcacgc gcagtcgcag ctcgccgcgc
     2101 tgcccactgc gctgcgcgcg ctcgacacgc cggcccctca ttacgaggtc atcgtggcgc
     2161 cggcgctgcg ggcgctggcc gaagaagtcg atcgtcgcac tcggcgggag gccggttgat
     2221 tcgggtcaat cgggcgaaat gggcggcgag tacgttgaaa tcgagttgaa cgttattgct
     2281 gggaaagatc gatcatgacg acgaaacgca gaacccgctt gcgccagccg ggtcccccgc
     2341 tcgagccggt cgtggacgtc gagacgtcgc gcatcgtgca gcacccggat ggctatttct
     2401 ggatgtccga ggacggcaag cgcgaaacgg ggccgttcgc atcgttcgac gatgcgctcg
     2461 cggacatgtt ggccacggcg gagtcggaat ccgaacccgg cgagagcctg caggaggccg
     2521 aggacgagat cggcatcgcc gactggatcg atcccgacac gcgcgagccc gccgaagagc
     2581 ggcggccgcg gatcgaggat cagtaagacg cgcgctctag accgcgatgt ccggcagcgc
     2641 gcgcgccgcc tgttcgtgtg cgacctggcg cggcccgacg gcggcgaggg cggtcagttc
     2701 ctcggccagg aattcggtca cgtcgtcggc ggtcaggacg ccgatcaggc agtcatgctc
     2761 atcgatcacc ggcagccggc gtattccgct gtcgcgcatg cggacgatcg cgtcgtaaac
     2821 cgcttccgtt tcgcgcgcga gcgcaagccc cttgcgcatc aggtcgcgcg cggtcacctc
     2881 cgcgggatcg acccgctgcg ccagcacgtc gatcaccaga tcgcgatcgg tgacgatgcc
     2941 gaccggaatc agcttggcgc cgaccgcatc ggcgacgatc acgtcgccga catgatggcg
     3001 ccgcatcagc tgcgccagct cgaccacgct cgtggcgcgt tcgcagtaca cgacctcgcg
     3061 ggtgcagatt tcgccgatgc gcatgatccg ttcctccgtc gacttacttg ttgcatggtg
     3121 gctgcgccgg cgcgcgccgc attgacgtac gccaacgcgg gtgccggtcc ggccagccgg
     3181 tgagcgagag catgcaactg caaggtcccg gcccgcgcgc ggcgcaggtg atgcggcagc
     3241 ccggcgcctt cgcgctgcgt gtcttgacgg gttttcgtgc caaccagggg ctgctgctcg
     3301 cgggcgcggt cgcgtattac gcgctgttgt cgatcgtgcc gctgctgatc ctcacggtga
     3361 tcgcgctgtc gcgcgtgatc gatccggccg aactgctgca gacgctcggc cgctacctgg
     3421 aatggctgat ccccgggcag tcgcagatca tcgtcgccga gctcggcaat ttccttgcgc
     3481 accgcgacgt gctgggctgg gtcctgctgg cgacgatgat cttcttcagt tcgctcgcgt
     3541 tcaccgtgct cgagaacgcg atgtgcgtga tcttccatca tcgggtggcg atccgccggc
     3601 gccgcttcct cgtgtcggcg ctgatcccgt actgctacat cctgtcgctg gccgtcggac
     3661 tgctgctggt gacgctggtc gcgggcagcc tgcaggcgat ggggcaggaa agcgtcgagt
     3721 tcctcggccg cacgtggtcg ctcggcggcg tgtcgggtgc gctgctgtac ctgctcggag
     3781 tcgccggcga gatcttcgtg ctcacttccg tgtacatggt gatgccggtc ggccggctgc
     3841 ggctcacgca cgccctgatc ggcgcagtca cggcgacgct gctgtgggag ctgacgcggc
     3901 acgtgctggt gtggtacttc gcgaccctct ctcatatcgg cacggtgtac ggatcgctga
     3961 cgaccgcgat cgtggtgctg ctcagcctcg aactcggatc gacgctgctg ctgttcggcg
     4021 cgcaggtgat cgccgaattc gagcggatcg gcacggcggc ctccgaagcg ccgtccgagc
     4081 cgatgcgcct cgacgcaggt tgagcatcgc gggaccggct ttcgcgcggg cccgcgcccg
     4141 gtcgatcggt ccgcgcagga ggcgcagatg aacttcagcc tcgaccagga cttccccgcc
     4201 gatccggcac ggctctgggc agcgcttggc cgtcgcgact acgtcgagca gaaatatcgc
     4261 tcactcggag cagcaacact gcgcattctg cgcttcgagg tcacccgcga ttcgatcgac
     4321 gtggaactgg agcgcagcgc gcccgtcgcg cgcaaagcat tgcccgcatg ggcacgaccg
     4381 ttcgtggcag atcggcaagt gatgcgccat cacacccggt ggcggcgcgc ggatgtcact
     4441 cgcatcgacg ccgaactcga catctggccc gtgggcctgc cggtgcatgc gcatggggtt
     4501 ggaacattga ccgggcggcc gcccggacgg acgaacatga cgctgcactt cgaggtcaag
     4561 tgccggctgc cggcagtggg agcgaccgcg gctcgactat tcgcggacca ggtcaaggaa
     4621 gcgctgcagg ccgatcacgc attcaccctc gactacctcg ccgcggacgc aagcccgctg
     4681 cagcgtccgc gatgagcatc gttccgcctc attgatcgga ctgcattgat ccgacccgtc
     4741 gcggcaggac gctgcttgat accatcggcg cgcgacgacc atgaatcggt tgccatccga
     4801 atcgcttggt gaagaactgc gccgcgccgc gcgcgaagca cccgagcgcc cgttcatccg
     4861 catgcgcacc ggcgagtgga gctacggcgc gctcgacgag gaaagcgacc gtctcgccac
     4921 cgggctgcag aggctcggag tggcgcacgg cgatcacgtc agcctgctcg cccccaactg
     4981 catcgagttc gtcgcgctgt ggtttgcgct ggccaagctg ggcgcggtgg cggccccggt
     5041 gaacacggca ttccgcggcg cggcgctggc agacgcgatc gaaatcgtgc gcagccggtt
     5101 gctcgtgctg cacgcggatc acgtccagca gttcgccgag gtatcgccgc ggctcgagca
     5161 catcgaacag gtggtcgtga tcggggagca gatcagtctg gaacggccgg tgctgacata
     5221 cgcgcgactg cgcgacgcgg tggcgctcgc cccgcccgta ccagtgcgcg cctcggatct
     5281 gtgcctgctg
//
LOCUS       DBNHBY010000382         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1443326, whole genome shotgun sequence.
ACCESSION   DBNHBY010000382 DBNHBY010000000
VERSION     DBNHBY010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1443326"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="AC16HB_09510"
     CDS             complement(<1..692)
                     /locus_tag="AC16HB_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5607065.1"
                     /translation="MESNQANAAALRAFRVLDYVAEQANGCAPAQIVAALDLPKQTVH
                     RIVAQLQLAGLLIREPGTRRVQLGARLTHFAEHVLMHGQASRERHAILKALVDDIGET
                     CNLTALAGTDIVYLDRVETAWPLRVLLAPGSHVPLHATATGKLLLSLLPKAQRERLLR
                     VLPLRALTPATIVEVRELRRELEETRRRRIGINRNEHLSGLIAVAVPVMLDRQRACAA
                     VAVQAPVGRMTL"
     gene            822..1793
                     /locus_tag="AC16HB_09515"
     CDS             822..1793
                     /locus_tag="AC16HB_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5607066.1"
                     /translation="MDRMLVSSRRSLLAASCALIAAATVGMPRTGAAQDRYPTHPIEF
                     VVPWGAGGGADQLARRLARLIEPELKVAVPVVNVPGATGNTGMTKMLTAAPDGHTMAV
                     LIGDTLATIAGGGGRWKLADVIPLGVMIRQPTGLYVKMDSKYKTFADLLADAKSNELK
                     VAILGFGSSDEILVNQIVAKGAKLRMVPFAAPGERYASILGGHADVLVEQAGDIRSFL
                     DGKQMRPLVFFADAPAPGFPTVPLAKDSGYPIVINQFRSIVVRAGSDPKHVAVLATAL
                     ENAARSPDFAKYLEDELAYPNSYIPAQDARAFIEKELKLIESNAQKK"
     gene            1825..2385
                     /locus_tag="AC16HB_09520"
     CDS             1825..2385
                     /locus_tag="AC16HB_09520"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5607067.1"
                     /translation="MHARLRAIIPHSVMLAVSALLYYAATLIDVPGGARGRIGPDAWP
                     KFIVGAMAALCVYEIVKRLVVGTSFTATGLTQGLNRPPDEADRELAGRAPDEHNAKLA
                     AGIALIAMYAVGVSHVGFFVGTALFLALFSWIGGVRRPLTLAAVGLAGAMVLLVIFMR
                     LAYVSLPIGEGPFKVVSLTLLRLIGV"
     gene            2395..>2575
                     /locus_tag="AC16HB_09525"
     CDS             2395..>2575
                     /locus_tag="AC16HB_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5607068.1"
                     /translation="MDILLNLLHGLANIAAPVNLAAMVIGLVLGMLVAVLPGLTLVMG
                     VVLALPFTYKMGVEPA"
BASE COUNT          383 a          862 c          912 g          418 t
ORIGIN      
        1 tcgagcgtca tgcggcccac cggagcctgc acggcgacgg ccgcgcatgc gcgctgccgg
       61 tcgagcatca ccggcaccgc gaccgcgatc agcccgctca ggtgctcgtt gcggttgatg
      121 ccgatgcgac ggcggcgcgt ttcctccagc tcgcgccgca gttccctgac ttcgacgatc
      181 gtcgccggcg tcagcgcgcg caggggcagc acgcgcagca ggcgctcgcg ctgcgccttc
      241 ggcaacagcg acagcagcag cttgccggtc gcggtcgcgt gcagcggtac gtgcgatccg
      301 ggcgccagca gcacgcgcag cggccacgcg gtctcgacgc ggtcgaggta gacgatgtcg
      361 gtgccggcga gcgcggtcag gttgcaggtc tcaccgatgt cgtcgaccag cgccttcaga
      421 atcgcgtggc gctcgcggct tgcctggccg tgcatcagca cgtgctcggc gaagtgcgtc
      481 agtcgcgcac cgagctgcac gcgccgggtg ccgggctcgc ggatcagcaa gccggcgagc
      541 tgcagctgcg cgacgatgcg gtggaccgtc tgcttcggca ggtccagcgc ggcgacgatc
      601 tgcgccggcg cgcaaccgtt ggcctgctcg gcgacgtagt cgagcacgcg gaacgcgcgc
      661 agcgcggcgg cgttggcctg gttgctttcc attgcagcag aaaacccgga tttcgggaca
      721 ccgatgcact gcaatcgcta ctatcttcga tcgccggcgc cctgtcacga cttgcgtcgc
      781 ggcgcgcggc gtggccgatg agaaccacac aaggagactg catggaccgt atgctcgtgt
      841 cgtcgcgacg ttcgctgctg gcggcgtcgt gcgccctgat cgccgccgct acggtgggga
      901 tgccgcgcac cggcgcggcg caggaccgct atcccacgca cccgatcgag ttcgtggtgc
      961 cgtggggcgc cggcggtggt gccgaccagc tcgcgcgccg gctggcccgg ctgatcgagc
     1021 cggaactgaa ggtcgcggtg ccggtggtca acgtacccgg cgccacgggc aacaccggga
     1081 tgaccaagat gctgaccgct gcgcccgacg ggcacacgat ggcggtgctc atcggcgaca
     1141 cgctggcgac catcgcgggc gggggcgggc gctggaagct cgccgacgtg atcccccttg
     1201 gcgtgatgat tcgccagccg accggtctgt acgtgaagat ggacagcaag tacaagacct
     1261 tcgccgacct gctggccgac gcgaagtcca acgagctgaa ggtggccatc ctcggattcg
     1321 gcagctccga cgagatcctg gtcaaccaga tcgtcgccaa gggcgccaag ctgcgcatgg
     1381 tgccgttcgc ggcgcccggc gagcgctacg catcgattct cggcggccac gccgacgtgc
     1441 tggtcgagca ggccggcgac atccgttcct tcctggacgg caagcagatg cgcccgctgg
     1501 tgttcttcgc cgatgcgccg gcgccggggt ttccgaccgt gccgctggcc aaggacagcg
     1561 gatatccgat cgtgatcaac cagttccgct cgatcgtcgt tcgtgcgggc agcgatccga
     1621 agcatgtcgc cgtgctcgcg accgcgctgg agaacgcggc gcgctcgccc gacttcgcca
     1681 agtacctcga agatgaactc gcctatccga acagttacat cccggcgcag gatgcgcgcg
     1741 cgttcatcga gaaggagctg aaactgatcg agtcgaacgc gcagaagaaa tagaccgcgc
     1801 gaccgggaac tcagggagag tgcaatgcac gcgcgcctgc gcgcgatcat cccgcattcc
     1861 gtcatgctgg cggtgtcggc gctgctgtac tacgccgcca cgctgatcga cgtgcccggc
     1921 ggcgcgcgcg ggcgcatcgg cccggacgcg tggccgaagt tcatcgtcgg cgcgatggcc
     1981 gcgctgtgcg tgtacgagat cgtcaagcgg ctcgtggtcg gcacatcgtt caccgccacg
     2041 ggtctgacac aggggctgaa ccgccctccc gacgaagccg accgcgaact ggccgggcgt
     2101 gcgcccgacg aacacaacgc caagctcgcg gccggcatcg cgctgatcgc gatgtacgcg
     2161 gtcggcgtgt cgcacgtcgg cttctttgtc ggcacggcgc tgttcctggc gctgttctcg
     2221 tggatcggcg gagtgcgccg accgctgacg ctggcggccg tcggacttgc cggcgcgatg
     2281 gtgctgctgg tgatcttcat gcgccttgcg tacgtgtcgc tgccgatcgg cgaaggcccg
     2341 ttcaaggtgg tgtcgctgac gctgctgcgg ctgatcggcg tctaagggcc ggcgatggac
     2401 attttgctca acctgctgca cggactcgcg aacatcgccg caccggtgaa cctggcggcg
     2461 atggtgatcg gcctcgtgct cggcatgctg gtcgccgtgc tgccggggct gacgctggtg
     2521 atgggcgtcg tgctggcgct gccgttcacc tacaagatgg gggtggagcc ggcgc
//
LOCUS       DBNHBY010000383         1919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1308788, whole genome shotgun sequence.
ACCESSION   DBNHBY010000383 DBNHBY010000000
VERSION     DBNHBY010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1308788"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1473
                     /locus_tag="AC16HB_09530"
     CDS             115..1473
                     /locus_tag="AC16HB_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HIR5607069.1"
                     /translation="MALHTTTKGLDLPLAGAPAPHLRTPTVARVALLGADFPGLKPSL
                     LVTVGDRVLRGQPLFEDKKNPGVRFTAPAAGTVQAIHRGERRAFTSLVIDVAADDGPD
                     AQVEFANFDGRPPEQLDGASVRALLAESGLWTALRTRPFSKVPALDAEPHAIFVTAID
                     TRPHAPVPAAALNGHGEFFALGVRCVECLTSGNVYVCHDERTQVPEFDSARIKVEEFR
                     GPHPAGLPGTHIHLLDPVDRDHSAWHVGYQDVVAIGVLFATGKLRVERTVSLAGPGVA
                     EPSLLRARLGASLDDLTRGALAPGAQRVISGSVLDGRTAMGEATGYLGRYHLQVSALP
                     EGRQRELFGWIAPGRDKFSLWNVVLGAFTRRPLALTTSTNGSARAMVPIGAYERVMPL
                     DILPTFLLRALITRDTERAAALGALELDEEDLALCTLVCPGKVEYGPLLRATLAKIEA
                     EH"
     gene            1475..>1919
                     /locus_tag="AC16HB_09535"
     CDS             1475..>1919
                     /locus_tag="AC16HB_09535"
                     /inference="COORDINATES: protein motif:HMM:NF015100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR5607070.1"
                     /translation="MSALRRWLDRAGKPFEKGGKLEKLHALYEAADTFFYSPAHVSTT
                     GAHVRDALDLKRMMILVVIATLPCVAMALYNTGLQANLAIDPAKAARLEGWRHDVIRL
                     LGVGYSPASVWACAVHGLLYFLPLYVVTMVVGLTWEVGFAIARKEE"
BASE COUNT          282 a          658 c          684 g          295 t
ORIGIN      
        1 cggccgccgt tgatgaccgt caagccgcgg agcgtccgcc tcgcctacgg ttagcgggtc
       61 cgcggctatc ggcattcaat gccggacggc catcgcggcg acccctgtgc cacgatggct
      121 ttgcatacga cgaccaaggg actcgatctg ccgctggcgg gtgctccggc gccgcacctg
      181 cgcaccccga cggtcgcgcg cgtcgcgctg ctgggcgccg acttcccggg gctgaagccc
      241 tcgctgctgg tgacggtcgg cgaccgcgtg ctgcgcgggc agccgctgtt cgaggacaaa
      301 aagaatcccg gcgtgcgctt caccgcgccc gcggcgggca cggtgcaagc gatccatcgc
      361 ggcgaacggc gcgcgttcac gtcgctcgtg atcgacgtcg ccgccgacga cggccccgac
      421 gcgcaagtcg aattcgcgaa cttcgacggc cgtccgccgg aacaactcga cggcgcgtcc
      481 gtgcgcgcgc tgctggccga atcgggtctg tggaccgcgc ttcgcacgcg gccgttcagc
      541 aaggtgccgg cactcgacgc cgagccgcac gcgatcttcg tcaccgcgat cgacacgcgt
      601 ccgcatgcgc cggtgccggc cgcggcgctg aacggccacg gcgaattctt cgcgctcggc
      661 gtgcgctgtg tcgagtgcct gaccagcggc aacgtctacg tgtgccatga cgagcgcacg
      721 caggttccgg agttcgacag cgcccggatc aaggtcgagg aattccgcgg cccgcatccg
      781 gccgggctgc cgggcacgca catccacctg ctggacccgg tcgatcgcga ccacagtgcg
      841 tggcacgtcg gctatcagga cgtcgtggcg atcggcgtcc tgttcgcgac gggaaagctc
      901 agggtggaac gcaccgtgtc gctcgcggga cccggcgtgg ccgagccgag cctgctgcgg
      961 gcacggctcg gcgcatcgct cgacgacctg acgcgcggcg cgctggcgcc gggtgcgcag
     1021 cgcgtgatca gcggctccgt gctcgacggc cgcacggcga tgggcgaggc caccggctac
     1081 ctcgggcgct atcacctgca ggtcagcgca ttgcccgaag gccgacagcg cgaactgttc
     1141 ggctggattg cgccgggacg cgacaagttc tcgctgtgga acgtggtgct gggggcgttc
     1201 acgcgtcgtc cgctggcgct cacgaccagc accaacggct cggcgcgcgc gatggtgccg
     1261 atcggtgcgt atgagcgggt gatgccgctc gacatcctgc cgactttcct gctgcgcgcg
     1321 ctgatcacgc gggacacgga acgcgcggca gcactcggcg cgctcgaact cgacgaggaa
     1381 gacctcgcgc tgtgcacgct ggtctgtccc ggcaaagtcg aatacgggcc gctgctgcgc
     1441 gcaacgctgg cgaagatcga ggcggagcac tgagatgtcg gcattgcgcc gctggctcga
     1501 ccgcgccggc aagccgttcg aaaagggcgg caagctggaa aagctgcatg cgctgtacga
     1561 ggcggccgat accttcttct actcgccggc gcacgtgtcg accaccggtg cgcacgtgcg
     1621 cgacgcgctc gacctgaagc gcatgatgat cctggtcgtg atcgccacgc tgccgtgcgt
     1681 ggcgatggcg ctgtacaaca ccgggctgca ggcgaacctg gccatcgatc ccgcgaaggc
     1741 cgcacggctc gaaggctggc gccacgacgt gatccgtctg ctcggcgtcg gctactcgcc
     1801 ggcgagcgtg tgggcctgcg cggtgcacgg gctgctctac ttcctgccgc tgtatgtggt
     1861 gacgatggtc gtcgggctga cgtgggaggt cggcttcgcg atcgcgcgca aggaagaag
//
LOCUS       DBNHBY010000384         5783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1714629, whole genome shotgun sequence.
ACCESSION   DBNHBY010000384 DBNHBY010000000
VERSION     DBNHBY010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1714629"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="AC16HB_09540"
     CDS             <1..838
                     /locus_tag="AC16HB_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607071.1"
                     /translation="RVDAIVHLRANFAERLRRPDGAPIQVLINGVNANSANIVQGYLE
                     GAWSTWLTQRAQRSGVDLQVPVRVESRVWFNSELRSRNYLVPGLVVVIMTLIGALLTS
                     MVMAREWERGTMEALMVTPVGVTEILLGKLIPYFLLGTGGMLLSVAMAVWLFDVPLRG
                     SFWLLLLTASLFLLVALGMGLLISSVAKNQFIAGQAALIATFLPAFLLSGFIFDIGSM
                     PAVVQAITHVVAGRYFVAIATTLFLAGDVGSVIVPNAAALVLMAVIFLVLTWRKTRKR
                     LE"
     gene            843..1952
                     /locus_tag="AC16HB_09545"
     CDS             843..1952
                     /locus_tag="AC16HB_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607072.1"
                     /translation="MWQRIVALMRKEFLAVLRDRRSRFVLILPPLIQLLVFGYAATFD
                     LNNISYAVYDEDKGAAARQLAAAFRGSDRFSEVATITSIVEIAPLVDSRRVMVVVHIG
                     PQFTRDLTVGQPAPLQVIVDGRNSNSALVALNYVRRIVNGFNEEWIAARNLPQPPARL
                     QTRAWFNPNLESRWFFIPGLAGVITLLVSLLVTALSVAREREQGTFDQLLVTPLRPVE
                     ILIGKALPGFIIGFIEATVIILAATLWFDIPLRGSLVVLYVGLALFLLSAVGVGLMLS
                     SLAVTQQQGLLGAFLFMVPAIILSGFATPIANMPRAVQLITLLDPLRYFLVILRKVFL
                     EGVSFDVLLPQMWPMAVIGVVALACAGWLFRHRMY"
     gene            2166..3593
                     /locus_tag="AC16HB_09550"
     CDS             2166..3593
                     /locus_tag="AC16HB_09550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5607073.1"
                     /translation="MKFASLGLSDALVRATSDVGYTEPTPIQLQAIPAVLAGRDMMAG
                     AQTGTGKTAGFVLPILQRLAAAPAAPKRTIRALILTPTRELAAQVEESVRVYGKHVPL
                     KSTVVFGGVSINPQITTLRHGVDVLVATPGRLLDHHGQRTVDLSHVEVLVLDEADRML
                     DMGFLPDIKRVLAALPRKRQNLLFSATFSDEIRGLAQGLLHDPLMIQVAQRNSTVETI
                     AQKVLPVEGKQKAALLLHLVREHNWYQVLVFTRTKHGANRLATQLDKHGVPALAIHGN
                     KSQNARTRALAEFKSGKLQVLVATDIAARGLDIDNLPHVVNFDLPHVPEDYVHRIGRT
                     GRAGASGEAISLVAPEERDLLRDIERVIRRQLPREVVTGFAPLSAPAEADRGPRPSRA
                     AHAPHPQRRAGAHAPRREAPAHPPRHAAPAHPAPRQAVPQPKHRDGDATKRAAHPAPR
                     SRQPDADTRTRASLPPGWPGAAKRG"
     gene            3604..4230
                     /locus_tag="AC16HB_09555"
     CDS             3604..4230
                     /locus_tag="AC16HB_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5607074.1"
                     /translation="MLIYVADPMCSWCYGFAPELQALLDAMPAQELRLVMGGLRAYNT
                     RPMDAALRAALAHHWTEVERASGQPFDRGLLAREDFVYDTEPACRAVVTARSIEPSFA
                     WPLLHAIQRAFYAQGRDVTRADVLTEIHGEAASRSAEGFAQAFGSEAMKEATRADFER
                     AREWEVQGFPTLFAEHAGHYALLARGYTKADTLIERARAFFNDAGRSP"
     gene            complement(4205..5299)
                     /locus_tag="AC16HB_09560"
     CDS             complement(4205..5299)
                     /locus_tag="AC16HB_09560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5607075.1"
                     /translation="MTLRFGSRTARIALLMLALLGASSALVLAQNPPRAEPRAVAPRG
                     PLAAHEQSVVSLFEASAPSVAYITTEVVQRTGLFTAEVGQGAGSGFVWDGAGHVVTNY
                     HVVEGARRVFVQLDAGEPIEAKPVGVAPEYDLAVVRLNRVPKNLRPIPLGTSRDLKIG
                     QSVYAIGNPFGLQRTLTQGIVSALDRELPTIEGYREVAGVIQTDAAINPGNSGGPLLD
                     SAGRLIGVNSAIRSASGSSAGVGFAIPVDLVNRIVPALIARGRAPLPGIGIVPVRPDL
                     VARAGIAGIVIDQITRGTPAAEAGLRPLNRQSGDPGDVIVAVNGRRVETLSSFAAELD
                     RVGVDNSAELTVSRDGKERNVRVRVIDLRR"
     gene            5402..5689
                     /locus_tag="AC16HB_09565"
     CDS             5402..5689
                     /locus_tag="AC16HB_09565"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607076.1"
                     /translation="MPPNDYRIEHNAAAGRFETRIDGWLCRCDYRLVDGILHLIHTEV
                     APALEGRGIAAALVRAALAWAQDQGLKVLPRCSYVRAYMRRHPETQALRAQ"
BASE COUNT          880 a         2006 c         1955 g          942 t
ORIGIN      
        1 ccgggtcgac gccatcgtgc acctgcgcgc gaacttcgcc gagcggctgc ggcggcccga
       61 cggcgcgccg atccaggtgc tgatcaacgg cgtcaacgcc aactccgcga acatcgtgca
      121 gggctacctc gaaggcgcct ggtccacgtg gctgacgcag cgcgcgcagc gcagcggcgt
      181 cgacctgcag gtgcccgtcc gcgtcgagtc gcgggtgtgg ttcaacagtg aattgaggag
      241 ccgcaattac ctggtgccag ggctcgtcgt ggtgatcatg acgctgatcg gcgcgctgct
      301 cacctcgatg gtcatggcgc gcgaatggga acgcggcacg atggaggcgc tgatggtcac
      361 gccggtgggc gtcacggaaa tcctgctcgg caagctgatt ccgtattttc tcctcggcac
      421 cggcggcatg ctgctttcgg tggcgatggc ggtctggctc ttcgacgtgc cgctgcgcgg
      481 ttcattctgg ctgctgctcc tgacggcgtc gctgttcctg ttggtcgcgc tgggcatggg
      541 actgctgatc tccagcgtgg cgaagaacca gttcatcgcc gggcaggccg cgctgatcgc
      601 gacgttcctg ccggctttcc tgctgtcggg tttcatcttc gacatcggca gcatgcccgc
      661 cgtggtgcag gcgatcacgc atgtggtggc cggccgctac ttcgtcgcga tcgccaccac
      721 gctcttcctt gccggcgacg tcgggtcggt gatcgtgccc aacgcggcag cactggtgtt
      781 gatggcggtg atcttcctcg tcctgacctg gcgcaagacc cgcaagcgcc tggagtagcc
      841 gcatgtggca gcggatcgtc gcgctgatgc gcaaggaatt ccttgcggtg ctcagggacc
      901 gcaggagccg cttcgtgctg atcctgccgc cgctgattca gttgctcgtg ttcggttacg
      961 ccgccacgtt cgacctgaat aacatctcct atgcggtgta cgacgaggac aagggcgccg
     1021 cggcgcgaca actggcggcc gcgttccgcg gctccgatcg gttcagcgag gtggcgacga
     1081 tcacctccat cgtcgaaatc gcgccgctgg tggacagccg gcgcgtgatg gtggtggtgc
     1141 acatcggccc gcagttcacg cgcgacctga cggtcggcca gcccgcgccg ctgcaggtga
     1201 tcgtcgacgg gcgcaattcc aacagcgcgc tggtcgcgct caactatgtg cgccggatcg
     1261 tcaacgggtt caacgaggag tggatcgccg cgcgcaacct gccgcagccg ccggcgcgac
     1321 tgcaaacccg cgcctggttc aacccgaacc tggagagtcg ctggttcttc attcccgggc
     1381 tggccggcgt catcacgctg ctcgtctcgc tgctcgtcac cgcgctgtcg gtggcgcgcg
     1441 aacgggaaca aggcaccttc gatcagcttc tggtcacgcc gctgcgcccc gtggaaatcc
     1501 tgatcggcaa ggcgctgccg ggcttcatca tcggattcat cgaggccacc gtgatcatcc
     1561 tcgcggcaac gctttggttc gacatcccgc tgcgaggcag cctggtggtg ctgtacgtcg
     1621 ggctcgcgct attcctgctt tccgcggtgg gcgtcgggct gatgctgtcg tcgctcgcgg
     1681 tgacgcagca gcaaggcctg ctcggcgcgt tccttttcat ggtgccggcg atcatcctgt
     1741 ccggtttcgc cacgccgatc gcgaacatgc cgcgcgcggt gcagttgatc acgctgctcg
     1801 atccgctgcg ctacttcctg gtcatcctgc gcaaggtgtt cctcgagggc gtgtcgttcg
     1861 acgtgctgct gccgcagatg tggccgatgg cagtgatcgg cgtggtcgcg ctcgcctgcg
     1921 cgggctggtt gtttcgtcac cgcatgtact gaggccgcgc ggaccgtcga cctgctacac
     1981 tgcgttcacg cggcatgtga ccagcgccgt cgattcccga tcgacgtgcc tttgccgctg
     2041 tcaatcctga cggggccgat tcgaagtacc ggctccaact gattacccgt ttgtctgcaa
     2101 tcggctggtc gatgaacgct gtggcgcgat gcgtccgtgc ggcaggctga ccaccgaaag
     2161 caaagatgaa attcgcaagc cttggcttgt ccgacgccct cgtgcgcgcg acgagcgatg
     2221 tcggctatac cgaaccgaca cccattcaac tgcaggcaat tcctgctgtc ctcgctggcc
     2281 gcgacatgat ggccggcgcg cagaccggca ccggcaagac cgccggcttc gtgctgccga
     2341 tcctgcagcg cctggcggcg gcgcccgccg cacccaagcg caccatccgc gcgctgatcc
     2401 tgacgccgac gcgtgaactc gccgcgcagg tcgaggaaag cgtgcgcgtg tacggcaagc
     2461 acgtgccgct gaaatcgacc gtcgtcttcg gcggcgtgtc catcaatccg cagatcacca
     2521 cgctgcggca cggcgtcgac gtgctggtgg ccaccccggg ccgcctgctg gatcaccacg
     2581 gccagcgcac cgtagacctt tcgcacgtcg aagtgctggt gctcgacgaa gccgatcgca
     2641 tgctcgacat gggcttcctg cccgatatca agcgcgtgct ggcggcgctc ccgcgcaagc
     2701 ggcagaacct gcttttctcg gccacgttct ccgatgagat ccgcggcctc gcgcaggggc
     2761 tgctgcacga tccgctgatg atccaggtcg cccagcgcaa cagcacggtc gagacgatcg
     2821 cgcagaaggt gctgccggtc gaaggcaagc agaaggccgc gctgctgctg cacctggtgc
     2881 gcgaacacaa ctggtaccag gttctggtgt tcacgcgcac caagcatggt gcgaaccggc
     2941 tcgcaaccca gctcgacaag cacggcgtgc cggcgctggc gatccacggc aacaagagcc
     3001 agaacgcgcg cacgcgcgcg ctggccgagt tcaagagcgg caagctgcag gtgctggtcg
     3061 ccaccgacat cgccgcgcgc gggttggaca tcgacaacct gccgcacgtg gtgaacttcg
     3121 acctgccgca cgtaccggag gattacgtgc accgcatcgg ccgcaccggc cgcgccggcg
     3181 ccagcggcga ggcgatctcg ctggtcgcgc cggaagagcg cgacctgttg cgcgacatcg
     3241 agcgcgtgat ccggcgtcag ttgccacgcg aggtagtgac cggcttcgcg ccgctcagcg
     3301 ccccggccga ggccgaccgc gggccgcgtc cgtcgcgcgc cgcgcatgcg ccgcacccgc
     3361 agcgccgggc aggcgcgcac gcgccgcggc gtgaggcgcc cgcccatccg ccgcgacacg
     3421 cggcgcccgc tcaccctgcg ccgcgtcagg cggtgccgca gcccaagcac cgcgatggcg
     3481 acgccacgaa gcgcgcagcg catccggcac cgcgatcgcg gcagccggat gccgatacac
     3541 ggacccgcgc cagcctaccg cccggctggc ccggcgcggc caagcgcgga tgatcaggac
     3601 tcgatgctga tctacgtcgc cgatccgatg tgctcgtggt gctacggctt cgcacccgaa
     3661 ctgcaggcgc tgctcgacgc gatgcctgcg caggaactgc gactggtgat gggcgggctg
     3721 cgcgcgtaca acacgcggcc gatggacgcg gcgctgcgcg ccgctctcgc ccaccattgg
     3781 acggaagtgg aacgcgccag cggacagccg ttcgatcgcg ggctgctggc gcgcgaggac
     3841 ttcgtctacg acaccgagcc agcgtgccgc gcggtcgtca ccgcccgcag catcgagcct
     3901 tcatttgcct ggccccttct gcacgcgatc cagcgcgcgt tctatgcgca gggacgcgac
     3961 gtgacgcgcg ccgacgtgct gaccgagatt catggcgaag ccgcgagccg ctccgccgaa
     4021 ggattcgcac aggctttcgg cagcgaggcg atgaaggaag ccacgcgcgc cgacttcgag
     4081 cgcgcgcgcg aatgggaggt gcagggattc ccgacactgt tcgccgagca tgccggccac
     4141 tacgcgctgc tcgcgcgcgg ctacacgaag gccgacacgc tgatcgaacg cgcgcgcgcg
     4201 ttcttcaacg acgcaggtcg atcaccctga cgcgcacgtt gcgctccttg ccgtcgcggc
     4261 tgaccgtcag ctccgcgctg ttgtcgacgc cgacgcggtc gagttcggcg gcgaacgacg
     4321 aaagcgtctc gacgcgccgg ccgtttacgg caacgatcac atcgccgggg tcgccgctct
     4381 ggcgattcag cggccgcagt cccgcctcag ccgcgggcgt gccgcgcgtg atctggtcga
     4441 tcacgatgcc ggcgatgccc gcgcgcgcga ccagatccgg ccgcaccggc acgatgccga
     4501 tgccgggcag cggcgcccgg ccgcgcgcga tcagcgcagg gacgatgcga ttgacgaggt
     4561 cgaccgggat cgcgaaaccg acgcccgccg aactgccgct cgccgagcgg atcgcggagt
     4621 tcacgccgat caatcgcccg gcgctgtcga gcagcggacc gccggagttg ccgggattga
     4681 tcgccgcgtc ggtctggatc acgccggcga cttcacgata gccctcgatc gtcggcagct
     4741 cgcggtcgag cgcgctgacg atgccctgtg tcagcgtgcg ctgcaggccg aagggattgc
     4801 cgatcgcgta caccgactgg ccgatcttca ggtcgcgcga agtgccgagc ggaatcggcc
     4861 gcaggttctt cggcacgcgg ttcaggcgca ccaccgcgag atcgtattcg ggcgccacgc
     4921 cgaccggctt cgcttcgatc ggttcgccgg cgtcgagctg cacgaacacg cgccgcgcgc
     4981 cttcgaccac gtggtagttg gtcaccacgt ggccggcacc gtcccatacg aagccgctgc
     5041 ccgcgccctg ccccacctcg gcggtgaaca agccggtgcg ctgcacgacc tcggtcgtga
     5101 tgtaggcgac cgacggcgcc gacgcctcga acaggctgac cactgactgc tcgtgcgcgg
     5161 caagcggccc gcgcggtgcc accgcgcgcg gctcggcgcg cggcggattc tgcgccagca
     5221 ccagcgccga tgaagcgccg agcaatgcaa gcatcagcag cgcgatgcgc gccgtgcgcg
     5281 acccgaatcg aagtgtcata gttcccggtc ctcgctcatg gtgccgctgc agatgggagc
     5341 cgccggtggc aatttcaacc cgcgccgagc gtgcgttacg cttgcgcgcc gaggattcga
     5401 catgccaccg aacgattacc ggatcgagca caacgcggcc gccggccgct tcgagacgcg
     5461 catagacggc tggctgtgcc gttgcgacta ccggctcgtc gacggcatcc tgcacctgat
     5521 tcacacggaa gtcgcgccgg ccctcgaagg ccgcggcatc gcggccgcgc tggtgcgcgc
     5581 cgcgctggca tgggcgcagg accagggcct gaaggtgctg ccgcgctgct cgtacgtgcg
     5641 cgcgtacatg cgacggcatc cggaaactca ggcgctgcgc gcacagtaac ggagagacca
     5701 cgatgatcgc gcgacgactg ctgctggccg cgtctgcgac cggactgctc gccgcatgcg
     5761 gcggcggggg gggggggggg ttg
//
LOCUS       DBNHBY010000385         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_22462, whole genome shotgun sequence.
ACCESSION   DBNHBY010000385 DBNHBY010000000
VERSION     DBNHBY010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_22462"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC16HB_09570"
     CDS             complement(<1..146)
                     /locus_tag="AC16HB_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5607077.1"
                     /translation="MSDYHDFDHFGLDPLIVAAVKEMGYTTPTPIQSKAIPVVLKGRD
                     VMGAA"
     gene            complement(163..239)
                     /locus_tag="AC16HB_09575"
     tRNA            complement(163..239)
                     /locus_tag="AC16HB_09575"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Ile,seq:cat)
     gene            complement(282..2522)
                     /gene="rpoD"
                     /locus_tag="AC16HB_09580"
     CDS             complement(282..2522)
                     /gene="rpoD"
                     /locus_tag="AC16HB_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR5607078.1"
                     /translation="MTKGTLAGKKLVHAAAKAPKVSKAAVRPQARTADNGSGAKKAAA
                     LESGRELKAKGTPLAPVVDSSAKGRPGRKPKQQTDTPMGGDGSADAVPEAGDDTVDPS
                     VQVVEKLSVKQRAKDRRAKEKALKEALERSYHGTVEDLEARRNKLKALIKLGKERGFL
                     TYAEINDHLPDNLVDAEAIDAIISTFGDMGIQVFDQAPDAETLLMTENAPAATSEMDA
                     EEEAEAALSTVDAEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEDGLKHMVMAI
                     SACPTTIAEILSHAEKIRGVELPIDEIVDGLVDESDDFPARGSAAAEEDDEGEETTDI
                     GASGMTAGQLEELKARALEKFDHVAKLFDRMRVAFEKEGYKSPGYVRAQQKILDELMS
                     LRFTAKMVERLCDSLRAQVEEVRSLEKQIYEIMVNKCGMPRPHFLKHFPGAETNTRWV
                     TREADGGHPYSEVLARNLPAVHELQGKLSDLQKRVVLPLKDLREVYKQMATGEAKARK
                     AKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
                     WWLRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGTEPDPATLAVKMEMP
                     EDKIRKILKIAKEPISMETPIGDDDDSHLGDFIEDTSTVAPADAAQNSSLAGVTKEVL
                     DSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSDK
                     LKSFLE"
BASE COUNT          361 a          859 c          788 g          553 t
ORIGIN      
        1 gcggcgccca tcacgtcgcg tcccttgagc acgacgggaa tcgccttgct ctggatcggc
       61 gtgggcgtcg tgtagcccat ctccttgacc gccgcgacga tcagcggatc aaggccgaaa
      121 tggtcgaaat cgtgatagtc ggacaagcgt tctgcggttc agtggtgggc cgtgttggac
      181 tcgaaccaac gaccaaggga ttatgagtcc cctgctctga ccgactgagc taacggccca
      241 ttcggcgagc agcgtattcg atcgcgcgcg ctcacgtcga attactccag gaagctcttc
      301 agcttgtcgg agcggctcgg gtgccgcagc ttgcgcagcg ccttggcctc gatctggcgg
      361 atgcgttcgc gcgtgacgtc gaactgcttg ccgacctctt ccagcgtgtg atcggtcgac
      421 atctcgatgc cgaagcgcat gcgcagcacc ttggcttcgc gcggcgtcag cgaatcgagc
      481 acttccttgg tcacgccagc aaggctcgag ttctgcgccg cgtcggccgg ggccaccgtc
      541 gaggtgtcct cgatgaagtc gcccaggtgc gaatcgtcgt cgtcgccgat cggcgtctcc
      601 atgctgatcg gttccttggc gatcttcagg atcttgcgga tcttgtcttc cggcatctcc
      661 atcttgaccg ccagcgtggc cggatcgggc tcggtgccgg tttcctgcag gatctggcgg
      721 ctgatccggt tcatcttgtt gatcgtctcg atcatgtgca cgggaatgcg gatcgtgcgc
      781 gcctggtcgg cgatcgagcg cgtgatcgcc tgccggagcc accacgtcgc gtaggtcgag
      841 aacttgtagc cgcggcggta ctcgaacttg tcgaccgcct tcatcagtcc gatattgcct
      901 tcctggatca ggtcgaggaa ctgcagcccg cggttggtgt acttcttcgc gatcgagatc
      961 acgaggcgca ggttcgcctc ggtcatctcg cgcttggcct tgcgcgcctt cgcttcgccg
     1021 gtggccatct gcttgtagac ctcgcgcagg tccttcagtg gcaacacgac gcgcttctgc
     1081 aggtcggaga gcttgccctg cagctcgtgg acggccggca ggttgcgtgc cagcacttcg
     1141 gaatacgggt ggccgccgtc ggcctcgcgc gtgacccagc gcgtgttggt ttccgcgccg
     1201 gggaagtgtt tcaggaagtg cgggcgcggc atcccgcact tgttgaccat gatctcgtag
     1261 atctgcttct cgaggctgcg cacttcctcg acctgcgcgc gcagcgagtc gcacagccgc
     1321 tcgaccatct tggcggtgaa gcgcaggctc atcagctcgt cgaggatctt ctgctgcgcc
     1381 ctgacatacc ccggcgactt gtagccctcc ttctcgaagg caacgcgcat cctgtcgaaa
     1441 agcttggcga cgtggtcgaa cttctcgagc gcccgcgcct tgagttcctc gagctggccc
     1501 gcggtcatgc cgctggcgcc gatgtcggtg gtctcctcgc cctcgtcgtc ctcttcggcc
     1561 gccgccgacc cacgcgccgg gaaatcgtcg gactcgtcga cgaggccgtc gacgatttcg
     1621 tcgatcggca actcgacgcc acgaatcttc tccgcgtgcg acaggatctc ggcgatcgtg
     1681 gtcgggcagg ccgaaatcgc catcaccatg tgcttcaggc cgtcctcgat ccgcttggca
     1741 atctcgatct cgccctcgcg cgtcagcagt tccaccgagc ccatctcgcg catgtacatg
     1801 cgcaccggat ccgtggtgcg gccgaattcg gcgtccaccg tcgacagcgc cgcctcggcc
     1861 tcttcctcgg cgtccatctc ggacgtggcg gccggcgcgt tctcggtcat caacagcgtt
     1921 tcggcatcgg gcgcctggtc gaagacctgg atgcccatgt cgccgaaggt cgaaatgatc
     1981 gcgtcgatcg cctcggcgtc gaccaaattg tccggcagat ggtcgttgat ctcggcgtag
     2041 gtcaggaagc cgcgctcctt gccgagcttg atcagcgcct tgagtttgtt gcggcgggct
     2101 tcgagatcct cgaccgttcc gtggtagctg cgctcgagcg cctccttcag cgccttttcc
     2161 ttcgcgcggc ggtccttggc gcgctgcttg accgagagct tctcgacgac ttgaacgctc
     2221 ggatcgaccg tatcgtctcc cgcctccggc accgcgtcgg cgctgccatc accgcccatt
     2281 ggggtgtcgg tctgctgctt gggtttgcgt ccggggcggc ccttggcgga cgaatcgaca
     2341 accggagcca gaggagtgcc cttggccttc aattcacggc cggactcaag cgcggccgct
     2401 ttcttcgccc ccgagccgtt gtccgccgtc ctggcctgcg gcctcaccgc cgcctttgaa
     2461 accttgggag ccttcgctgc cgcatgcacc agcttcttgc ccgcaagggt tcccttcgtc
     2521 atcgtcttga ccatccagcc acccgatcaa tgcctgtcca c
//
LOCUS       DBNHBY010000386         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1714713, whole genome shotgun sequence.
ACCESSION   DBNHBY010000386 DBNHBY010000000
VERSION     DBNHBY010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1714713"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="AC16HB_09585"
     CDS             <1..409
                     /locus_tag="AC16HB_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR5607079.1"
                     /translation="TDTEDPPRFVAILPLRQGANPVDYGAAVAAQQKRAYPDIEPARL
                     AIPPAQALARCEAVARALGWEIVAIDPAAGRIEATATTPLFGFKDDVVIRVTPRDRGS
                     RVDMRSTSRVGVSDLGTNAERVRAFMGRLQQQQ"
     gene            complement(522..1532)
                     /locus_tag="AC16HB_09590"
     CDS             complement(522..1532)
                     /locus_tag="AC16HB_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008273 - calcium, potassium:sodium
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006874 - intracellular calcium ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR5607080.1"
                     /translation="MAAELFIWLQLALAAGAIVIAGTKLSHYGDLIARRTGLSGGWIG
                     LVLLATVTSLPELTASVSAIVLAHAPEVAVGNVLGACVLSFAMIAVLDALHRSASIYS
                     VASQGHALGAGFALLMLGATGFALLLPSGESALIGHVNAVTLLLFLLYAVAIRTIYDY
                     ERRTLAQAPLPATPVVMVGLGGLLWRYGVAAAVVVAAALWLPFIASEIARQMHWTEGF
                     VGTLLVAASTTLPELAVTIAAVRLGALDLAIGNLIGSILFNLVVLGIGDIVYTPGSLY
                     AAVAPTHAISVVTAVMMAGAVIVALVARQQARLLNFVSWTSVALALLFFANAWIHLRQ
                     SP"
     gene            complement(1605..>2030)
                     /locus_tag="AC16HB_09595"
     CDS             complement(1605..>2030)
                     /locus_tag="AC16HB_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5607081.1"
                     /translation="AADYVLAHRSLFGPRPQFTLIHVVESPATARRAVRDEIAPLPDA
                     VDLERIERAAFERVVAPIRRRYAADGVNVQERFAVGSPAAEIARATEDERLDILVMGS
                     HGRGAFTAALLGSVAWRVAATCATPLLLIRREAMQPAPE"
BASE COUNT          346 a          734 c          686 g          264 t
ORIGIN      
        1 caccgacacc gaagacccgc cccgcttcgt cgcaatactg ccgctgcgcc agggcgccaa
       61 tccggtcgac tacggcgctg ccgtcgccgc gcagcagaag cgcgcctacc ccgatatcga
      121 accggcacgc ctcgcgatcc cgcccgcgca ggcgctcgca cggtgcgagg cggtggcgcg
      181 cgcgctgggc tgggagatcg tcgcgatcga tcctgccgcg gggcgcatcg aagcgaccgc
      241 caccacgccg ctgttcggct tcaaggacga tgtcgtcatc cgcgtcacgc cgcgcgaccg
      301 cggcagccgc gtcgacatgc gctcgacctc gcgcgtcggc gtgagcgatc tgggcaccaa
      361 cgccgagcgc gtgcgcgcgt tcatgggccg actgcagcag cagcagtgat cggtccgctg
      421 cccggcgcgg tggcgcgttc gttgcggcct cgggcggccg gaacgccgcg tcgaattgcg
      481 tggaaccacg ttgcgagcgg tttgctttgc tcacctcgcc gtcacggact ctgtcgcagg
      541 tggatccatg cattggcgaa gaacagcagc gccagcgcaa cgctggtcca gctgacgaag
      601 ttcagcagcc gcgcctgctg gcgcgcgacc agcgcgacga tcacggcgcc ggccatcatc
      661 accgcggtga cgaccgatat cgcatgcgtc ggtgcgacgg ccgcgtacag cgaccccggc
      721 gtgtagacga tgtcgccgat gccgagcacg accaggttga acaggatgct gccgatcaga
      781 ttgccgatcg ccaggtcgag cgcgccgagc cggaccgcgg cgatcgtcac ggccagctcg
      841 ggcagcgtgg tgctggcagc aacgagcagc gttccgacga acccctcggt ccagtgcatc
      901 tgccgcgcga tctcgctcgc gatgaacggc agccacaatg ccgccgcgac caccacggcg
      961 gcagcgacgc catagcgcca cagcaacccg cccaggccga ccatcacgac aggcgtggcg
     1021 ggcagcggcg cctgcgcaag ggtgcgccgt tcgtagtcgt agatggtgcg aatcgccacc
     1081 gcatagagca gaaacagcag cagcgtcacc gcgttgacat ggccgatcag cgcactttcg
     1141 ccggacggca gcagcagcgc gaagccggtc gcgccgagca tcagcagggc gaatccggca
     1201 cccagcgcat gaccctggct cgccacggaa tagatcgacg ccgagcgatg cagcgcgtcg
     1261 agcacggcga tcatcgcgaa gctcagcacg cacgcgccga gcacgttgcc gaccgcgacc
     1321 tccggcgcat gcgcgaggac gatggccgag acgctggcgg tcaactccgg cagcgaggtc
     1381 accgtcgcca gcagcacgag cccgatccaa ccgcccgaca gcccggtgcg gcgcgcgatc
     1441 aggtcgccgt agtgcgacag cttcgtgccc gcgatcacga tcgcgccggc cgccagcgca
     1501 agctggagcc agatgaaaag ttctgccgcc accttcagtc ccttcgtcgc aagcgacgga
     1561 ttagaccgcg atgcgctcgg cggcaacaga gcccgatgcc gcgctcactc tggcgccggt
     1621 tgcatcgctt cgcgtcgaat cagcagcagc ggtgtggcgc acgtggccgc cacgcgccac
     1681 gccaccgacc cgagcagcgc cgcggtgaag gcgccgcggc cgtgcgaacc catcaccagg
     1741 atgtcgaggc gttcgtcttc cgtcgcgcgc gcaatctcgg cggccggcga cccgacggcg
     1801 aatcgctcct gaacgttcac gccgtcggct gcgtagcggc gccggatcgg tgcgaccact
     1861 cgctcgaagg cagcgcgttc gattcgttcg agatcgaccg cgtcgggcag cggtgcgatt
     1921 tcgtcgcgca ccgcgcgccg cgccgtggcc ggcgactcga ccacgtggat cagcgtgaac
     1981 tgcgggcgcg gaccgaacag cgaacggtgc gcgagcacgt aatcggccgc
//
LOCUS       DBNHBY010000387         3052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1241489, whole genome shotgun sequence.
ACCESSION   DBNHBY010000387 DBNHBY010000000
VERSION     DBNHBY010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1241489"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1073)
                     /locus_tag="AC16HB_09600"
     CDS             complement(102..1073)
                     /locus_tag="AC16HB_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607082.1"
                     /translation="MKRRELLTAAALAPLAWRTFAQGAYPTKSIRFVVPFAPGGTSEI
                     VARSVAQEMTAQLGQTVYVENKAGGAGTIAMTEVAHSAPDGYTLILSHVGTFAVNPYA
                     MTRHPYDVNKEFAPIALLARVPNIFVCNAEFPARDFRQFLELVKRNPGKYNYGSAGNG
                     SAGHLAFEYLKLVTGTFILHIPYRGTGPQLQDLLAGRTEFASAGTPALLPHIKSGKLR
                     ALAVGTPQRIPALPDVPTVAELGYKDFETSQWYGIHVPAGTPREIVLKLNAEANKALK
                     SNHVTQRFAADNATAGIGSPEDYAEFIGREQVRWKTVVERAQIVIDS"
     gene            complement(1082..1834)
                     /locus_tag="AC16HB_09605"
     CDS             complement(1082..1834)
                     /locus_tag="AC16HB_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607083.1"
                     /translation="MDLQLIHRTALVTGASSGIGRAIARELAREGARLAITGRRRDRL
                     EELGGEIAAAGGARPAIIVHDALDDGYVEAVATQALAALETVEILVNNAGGSRPFKLD
                     AGEQQWHEALTLNFTRHRQLAHRLLDQMIAHRWGRIINITGKSEPEGINGAFCAKAAI
                     HAWAKGLSREVGRHGITVNCIPPGRILSDQILRNYTPEYRAWQSEHEIPVGRYGNPEE
                     LAALAAFLASPRASYITGAVIPVDGGLRRYQF"
     gene            complement(1834..2244)
                     /locus_tag="AC16HB_09610"
     CDS             complement(1834..2244)
                     /locus_tag="AC16HB_09610"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5607084.1"
                     /translation="MPSLLSRAAAADWSVVCLCAAWCRTCDAYRPDLAALAEKARDMR
                     FVWLDVEDDADWIADLEIETFPTLLVLARGTPLFFGALPPQIGVLERTLSALHAGEAR
                     ATALEADAAEAAREIAARLARSSLELCAEAPREP"
     gene            2284..2913
                     /locus_tag="AC16HB_09615"
     CDS             2284..2913
                     /locus_tag="AC16HB_09615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607085.1"
                     /translation="MSAPSFSNPRETWDARFAVDRYIFGTAPNVFLSKQRARLGPGMR
                     ALAVADGEGRNSVWLAQQGLDVTAFDIAPRGVEKARTLARRHGVAVDFRIASVEEWDW
                     TPEAFDVVAAIFVQFAAPPVRARLFEGMLRTLKPGGWLFLQGYTPRQLEYRTGGPPCA
                     EHLYTQELLRASFSAHEIVELTEQDEVLDEGTQHAGMSALIDCVVRKST"
     gene            2928..>3052
                     /locus_tag="AC16HB_09620"
     CDS             2928..>3052
                     /locus_tag="AC16HB_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5607086.1"
                     /translation="MNYRHLGRCGLKVSELSFGSWVTYGNQVDLRAARELMAAAF"
BASE COUNT          446 a         1041 c         1056 g          509 t
ORIGIN      
        1 cggctcgggt ccttgcaagc gaaatggtcc cctcgccgcg cctagttctg cgcccaatgc
       61 gaccccgccg cgaccgtcgg ggactctgag ataggttccg ttcacgagtc gatgacgatc
      121 tgcgcccgct cgactaccgt cttccagcgc acctgttccc tgccgatgaa ctcggcgtaa
      181 tcctccggcg agccgatgcc tgcggtcgcg ttgtcggccg cgaagcgctg cgtgacgtgg
      241 ttcgacttca gcgccttgtt cgcctccgcg ttgagcttca gcacgatctc gcgcggcgtc
      301 cccgccggca cgtggatgcc gtaccactgc gaggtttcga aatccttgta gccgagctcg
      361 gccaccgtcg gcacgtcggg cagcgccggg atgcgctgcg gcgtgccgac cgcaagcgcg
      421 cgcagcttgc cgctcttgat gtgcggcagc agggccggcg tgccggcgga cgcgaactcg
      481 gtgcggccgg ccagcaggtc ctgcagttgc ggccccgttc cgcggtaggg aatgtgcagg
      541 atgaaggtgc cggtgacgag cttcaggtac tcgaaggcaa gatgccccgc gctgccgttg
      601 ccggccgagc cgtagttgta cttgcccggg ttcctcttca ccagttcgag aaactgccgg
      661 aaatccctgg cgggaaactc ggcgttgcac acgaagatgt tcggcacccg cgccagcagg
      721 gcgatcggcg cgaactcctt gttcacgtcg tacggatggc gcgtcatcgc gtacgggttg
      781 accgcgaagg tgccgacgtg gctcaggatc agcgtgtagc cgtcgggcgc ggaatgcgcg
      841 acctcggtca tcgcgatcgt gcctgcgccg ccggccttgt tctcgacgta aacggtctgg
      901 ccgagttgcg cggtcatctc ctgcgcgacc gatcgcgcga cgatctccga cgtgccgccg
      961 ggcgcgaacg gtacgacgaa gcggatcgac ttcgtcgggt acgcgccctg cgcgaacgtg
     1021 cgccacgcca gcggcgcgag cgcggcggcg gtcagcaact ccctgcgttt catggcctct
     1081 cctagaactg atatcggcgc agcccgccgt cgacgggaat caccgcgccg gtgatgtagc
     1141 tcgcgcgcgg tgacgccagg aacgccgcga gtgcggcgag ttcctcggga ttgccgtaac
     1201 ggccgacggg aatctcgtgc tccgactgcc acgcgcggta ttcgggcgtg tagttgcgca
     1261 ggatctggtc gctgaggatc cgtcccggcg gaatgcagtt cacggtgatg ccgtgtcgcc
     1321 cgacctcgcg cgacagcccc ttggcccacg cgtggatcgc cgccttggcg cagaacgcgc
     1381 cgttgatgcc ttccggctcg ctcttgccgg tgatgttgat gatgcggccc cagcggtggg
     1441 cgatcatctg atccagcagt cgatgcgcca gttgtcggtg gcgcgtgaaa ttgagcgtca
     1501 gcgcctcgtg ccactgttgt tcgccggcgt cgagcttgaa cggccggctg ccgccggcgt
     1561 tgttgaccag gatctcaacc gtttccagtg cggccagcgc ctgcgtcgcg acggcctcga
     1621 cgtagccatc gtcgagggcg tcgtgcacga tgatcgccgg acgcgcgccg cccgccgccg
     1681 cgatctcgcc gccgagttcc tccaatctgt cgcgccggcg accggtaatc gccagccgcg
     1741 caccttcgcg cgccagttcg cgcgcgatcg cgcggccgat gccgctgctg gcgccggtga
     1801 cgagggcggt gcggtggatg agctgcaggt ccatcagggt tcgcgcggcg cttccgcgca
     1861 gagttcgagg ctcgaccgcg ccaggcgggc ggcgatctcg cgcgccgctt ctgcggcgtc
     1921 ggcttcgagc gcggtggcgc gcgcctcgcc cgcgtgcagc gcggagagcg tgcgctcgag
     1981 cacgccgatc tgcggcggca gcgcgccgaa gaacagcggc gtaccccgcg ccagcaccag
     2041 cagcgtcgga aaggtctcga tttcgaggtc ggcgatccag tctgcgtcgt cctcgacgtc
     2101 gagccagacg aagcgcatgt cgcgcgcctt ctcggcgagc gcggccagat cgggccgata
     2161 tgcatcgcag gtgcggcacc acgccgcgca caggcacacg accgaccagt cggccgcagc
     2221 ggcgcgcgaa agcagggaag gcatgcgggg attatcgcgc tcccgtccgc taatcttcgg
     2281 cacatgagcg caccgtcgtt ctccaacccg cgcgagacgt gggacgcgcg ttttgcggtc
     2341 gaccgctaca tcttcggcac cgcgcccaac gtattcctct ccaagcagcg cgcgcggctc
     2401 gggcccggga tgcgcgcact ggccgtggcc gatggcgagg gccgcaacag cgtgtggctc
     2461 gcgcagcagg ggctcgacgt gaccgcgttc gacatcgcgc cgcgcggcgt ggagaaggcg
     2521 cgcacgttgg cgcgccggca cggcgttgcg gtcgacttcc gcattgccag cgtggaggaa
     2581 tgggactgga cgccagaggc gttcgacgtg gtcgccgcga tcttcgtgca gttcgccgcg
     2641 ccgcccgtgc gtgcgcggct gttcgaagga atgctgcgta cgctgaagcc cggcggctgg
     2701 ctgttcctgc agggctacac cccgcggcag ctcgaatatc gcaccggcgg gccgccgtgc
     2761 gccgagcacc tgtacacgca ggagctgctg cgcgcttcgt tttccgcgca cgagatcgtc
     2821 gagctcaccg aacaggacga agtgctcgac gaaggaacgc agcacgcggg catgagcgcg
     2881 ctgatcgact gcgtggtgcg caaatccacg taaccgggaa ggttgccatg aactaccgcc
     2941 acctcggccg ctgcggcctc aaagtcagcg aactgtcctt cggctcctgg gtcacctacg
     3001 gcaaccaggt cgacctgcgc gcagcgcgcg aactgatggc cgccgcattc ga
//
LOCUS       DBNHBY010000388         6040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1172860, whole genome shotgun sequence.
ACCESSION   DBNHBY010000388 DBNHBY010000000
VERSION     DBNHBY010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1172860"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC16HB_09625"
     CDS             <1..561
                     /locus_tag="AC16HB_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607087.1"
                     /translation="FIWNERTHNVAPATIGIPLAAPPAPAETEAPPPAPASLPRTALT
                     QAAAPTPTASTTLRAAGALTGRWLRSQRMPLVAAIALLVTGIVIGVLIATVAQRQAGD
                     GSPVNVTVTDGLDRAGIGLVLLEITPWGEVFVDNRPGGVSPPLKELQLPAGRHAIEIR
                     NASGKAVTARVEIDPARPSKIQHTFK"
     gene            558..977
                     /locus_tag="AC16HB_09630"
     CDS             558..977
                     /locus_tag="AC16HB_09630"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HIR5607088.1"
                     /translation="MTRAAAVALLLAAALSGCGTLQPPPAPPATATSVAALYQRPAER
                     ALIMGLSLYEQAAFDSAEQSFQAALKGGLADKQDVATAYKYLAFIACAFNRIDECAQD
                     FRAAFGADDRFALTDAEIGHPTWGPVFKRVAAERGRK"
     gene            complement(994..1995)
                     /locus_tag="AC16HB_09635"
     CDS             complement(994..1995)
                     /locus_tag="AC16HB_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5607089.1"
                     /translation="MSVPATVTAPIKPTISIAGFKDVYQVPRVEEALADLQELGTGAN
                     DALRATYVKMIKSGGQRFTVKPSALPDIDALIEELPNFEEPLEDVRRQLALCLSSDDP
                     LGIEPILLLGDPGIGKTHFARRLAKLLGTGYNFVGMSSLTAGWILSGASAQWKNAKPG
                     KVFDTLVNGDYANPVLVIDEIDKAGGDQQYDPLGSLYTLLEHDTAREFIDEFAEVPID
                     ASDVVWIATANNLHTIPEPILNRVNVYEIEAPDEEGARRIAAGIYRELRNEHAWGRQF
                     PEALDRDVLDRLARLKPREMRRVMLTAFGNAKLAGRDEVLLADVTEERIARKPRIGF"
     gene            complement(2191..2862)
                     /gene="narL"
                     /locus_tag="AC16HB_09640"
     CDS             complement(2191..2862)
                     /gene="narL"
                     /locus_tag="AC16HB_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HIR5607090.1"
                     /translation="MNAPVASKQTVLVVDDHPMFRKGVRQLLTMAEGIEVVGEAGNRA
                     EALELARRFEPDLILLDLNLKGESGLDILNALKEEDPSRRVVMLTVSDAPDDLIGAIR
                     AGADGYLLKDMEPEDLLARVREALSGSTVIGEALAVRLASALRQEARDSTRTAARELK
                     DLTDRERAVLRCVAEGQSNKLIARALSITEGTVKVHVKHLLKKLGFRSRVEAAVWATE
                     IGLKQ"
     gene            complement(2859..4769)
                     /locus_tag="AC16HB_09645"
     CDS             complement(2859..4769)
                     /locus_tag="AC16HB_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR5607091.1"
                     /translation="MNAWNRSTLLRVSTVLGIIALLAIAVVITAAVFTERSGGKGAAI
                     NVAGSLRMQSYLLATRVAALDVAPAEREKLIRGEIDGFEARLTHPQLVAALPGLVGGK
                     DGELARAHQRIAEQWRSEIKPLALRAATDEAARATLLSQIHPFVAAIDRFVLQLESDL
                     ESRIHSLQVALGAALFVTLVLVISAVFLLDLQVFQPVRDLVRCAQAVRRGDFGARAEL
                     TGPDELGQLGADFNHMVEELGRLYGSLESQIAVKTADLEQKNRSLALLYETTRELAHP
                     PLRRDALQRVAESVRHVLGVEGVVICAREAGTRGGFPLAHAEETAGRTCYIVRCEGCD
                     DSTQIVWHDADSERGPARIASIPLLDGERSFGVMPLTLVAGSQLTAAQLELAQVIGRH
                     IGAALAAEAGRDEHRRLALFEERSAIARELHDSIAQSLTYTKIQLARLSALIGGGTAA
                     GSDEAREVVEELRTGVSTAYQQLRELLTTFRLQLSGKGLRGALAQVAGDLRARTQIDV
                     ELRDELVGVALSANEQIHVLQIVREALANVEHHARAGHAWVRVARHADEEAAIEVTIE
                     DDGIGIGAATSPRHHFGLSIMRDRARALGGTIDIGTRRPCGTFVRLRFVPIAALTRTA
                     TEASAGLEVAHR"
     gene            4983..>6040
                     /locus_tag="AC16HB_09650"
     CDS             4983..>6040
                     /locus_tag="AC16HB_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607092.1"
                     /translation="MANVAVSLPSASPMSALRSRQWSVLIASTLGFTVCFAVWMMFAV
                     IGVPIKKTLDLNATQFGLLTAMPVLTGSLIRVPLGMWTDKFGGRIVFFLLMLSTVVPI
                     WLISYATEFWQFLVLGLFVGLAGGSFSVGTPYVARWFERKQQGFAMGVFGAGNSGAAV
                     TKFVAPALLVAYGWTMVPQVYAVSMLIMAIAFWFFSYHDPKHTIESHITYRRQLTVLS
                     DARVWKYCQYYSIVFGGYVALSLWMVNYYVGEFGLDIRVAALLAACFSLPGGVLRAIG
                     GWMSDKWGAHKVTWWVMWVSWICLFLLSYPATSYTVQTLNGPITFTLAHNMWTFTFLM
                     AVLGVAWAFGKASVFKYIS"
BASE COUNT          916 a         2092 c         1985 g         1047 t
ORIGIN      
        1 ttcatctgga acgaacgcac gcataacgtc gcgccggcca cgatcggcat tccgctcgcc
       61 gcgcctccgg cgcccgccga gacggaggcg ccgccacccg ctccggcatc gctgccacgc
      121 acggcgctga cacaggccgc ggcgccgacg ccaacggcgt cgacgacgct gcgcgccgcg
      181 ggcgcactga ccggtcgctg gttgcgcagc cagcgcatgc cgctcgtggc cgcgatcgcg
      241 ctgctggtca ccggcatcgt gatcggggtt ctgatcgcga ccgtcgctca gcgacaggcc
      301 ggcgacgggt cgcctgtcaa cgtgacggtg accgacggac tcgaccgggc cggcatcggc
      361 ctcgtgctcc tcgagatcac gccgtggggc gaagttttcg tcgacaaccg tccgggcggg
      421 gtgtcgccgc cgctgaagga actgcaactg ccggcgggtc ggcacgcgat cgaaatccgc
      481 aatgcgagcg gcaaggcggt gaccgcgcgc gtcgagatcg atccggcccg gccgagcaag
      541 atccagcaca cgttcaaatg acccgcgccg cggccgtcgc gctgctgctg gccgccgcgt
      601 tgagcggttg cggcacgctg cagccgccgc ccgcaccgcc ggcgacggcg acgagcgtcg
      661 cggcgcttta tcagcggccg gcggagcgcg cgctcatcat ggggttgtcg ctgtacgagc
      721 aggccgcgtt cgacagcgcc gagcagtcat tccaggcggc gctgaagggc gggctcgccg
      781 acaagcagga cgtggcgacc gcgtacaagt acctcgcctt catcgcctgc gcgttcaatc
      841 gcatcgacga gtgcgcgcag gactttcgcg cggcatttgg cgccgatgac cgatttgcgc
      901 tcaccgatgc cgaaatcggt cacccgacct ggggtccggt gttcaagcgc gtcgccgccg
      961 agcgcggcag gaagtaggag cccggtccgg cagctagaac ccgattcgcg gtttgcgggc
     1021 gatccgttct tcggtgacgt cggcgagcag cacttcatcg cgcccggcga gcttggcgtt
     1081 gccgaacgcg gtcagcatca cacggcgcat ctcccgcggc ttcagccgcg ccagccgatc
     1141 cagcacgtca cggtccagtg cctcggggaa ttgcctgccc cacgcgtgct cgttacgcag
     1201 ttccctgtag atgccggcgg caatgcggcg cgcgccttct tcgtcgggcg cctcgatctc
     1261 gtagacgttg acgcggttca gaatcggctc cgggatcgtg tgcaggttgt tcgcggtcgc
     1321 gatccacacc acgtcggagg cgtcgatcgg aacctcggca aactcgtcga tgaattcgcg
     1381 cgcggtatcg tgctccagca gcgtgtagag gctgccgagc gggtcgtact gctgatctcc
     1441 gccggccttg tcgatctcgt cgatgacgag caccgggttg gcgtagtcgc cattgaccag
     1501 cgtgtcgaag accttgcccg gcttggcgtt cttccactgt gccgacgcgc ccgacaggat
     1561 ccagcctgcg gtcagcgaac tcatgccgac gaagttgtag ccggtgccca gcagcttcgc
     1621 caaccggcgc gcgaaatggg tcttgccgat cccgggatcg cccagcagca gaatcggttc
     1681 gatacccagc gggtcgtcgc tcgacaggca cagcgccaac tggcggcgga cgtcctccag
     1741 gggctcctcg aagttcggca gttcctcgat cagggcgtcg atgtccggca gcgccgacgg
     1801 cttgacagtg aagcgctggc cgccggactt gatcatcttc acgtaggtcg cgcgcagcgc
     1861 gtcattggcg cccgtgccga gttcctgcag gtcggccagc gcctcctcga cgcgcggcac
     1921 ctgatacacg tccttgaatc cggcgatcga aatcgtcggc ttgatcggtg cggtgactgt
     1981 ggcaggaacg ctcatttcga cctcccgtgg gaacgctgct ggtctgcaac ccggatgcat
     2041 ctgcactgag ttgcagcggc gccccgacaa gttctacgcg aacgcagagc ggggcatgca
     2101 cgatgacaag caagcttcct gccatgatcg tgcgaaaaga tcacgaaaaa ggcaatgcag
     2161 cactcagctt ggttgagtgc caagacccgg tcactgcttc agaccgatct cggtcgccca
     2221 tacggcggct tcgacgcgcg agcggaagcc gagcttcttc agcaggtgct tcacgtgcac
     2281 cttgaccgta ccctcggtga tggaaagagc gcgcgcgatc agcttgttgc tctggccttc
     2341 cgcaacgcag cgcagcacgg cgcgctcgcg gtccgtgagg tccttcagct cacgcgcagc
     2401 cgtgcgcgtc gaatcgcgcg cctcctggcg cagcgccgag gcgagccgca ccgcgagcgc
     2461 ctcgccgatc acggtcgagc cggacagcgc ttcgcgcacg cgcgccagca ggtcttcggg
     2521 ttccatgtcc ttcagcagat agccgtcggc gccggcacgg atcgcaccga tcaggtcgtc
     2581 cggcgcgtcg gacaccgtca gcatcaccac gcgccgcgac gggtcttcct ccttcagcgc
     2641 gttcaggatg tcgaggccgc tctctccttt cagattcagg tcgagcagga ttaggtccgg
     2701 ctcgaagcgg cgcgccaatt ccagcgcctc ggcccggttg ccggcctcgc cgaccacttc
     2761 gatgccctcg gccatcgtca agagctggcg gacgcccttg cggaacatcg gatggtcgtc
     2821 gaccaccagc acggtctgct tcgaagcgac gggagcgttc atcgatgcgc cacctcgagg
     2881 ccggcactcg cctcggtcgc cgtccgcgtg agcgcggcga tcggcacgaa gcgcagccgc
     2941 acgaaggttc cgcacggccg gcgtgtcccg atgtcgatcg tcccgccgag tgcgcgcgcg
     3001 cggtcgcgca tgatcgacag gccgaaatga tgccgcggcg acgtggcggc gccgatgccg
     3061 atgccatcgt cctcgatcgt cacctcgatc gccgcctcct cgtcggcatg ccgagccacg
     3121 cgcacccagg cgtggccggc gcgcgcgtga tgctcgacgt tggccagcgc ctcacgcacg
     3181 atctgcagca cgtggatctg ctcgttggcc gacagcgcca cgccgaccag ctcgtcgcgc
     3241 agctcgacgt cgatctgcgt acgcgcgcgc aagtcgccgg ccacctgcgc gagcgcgccg
     3301 cgcagcccct tgcccgacag ctgcagacga aacgtcgtca gcagttcgcg cagctgctgg
     3361 taagcggtcg acacgccggt gcgcagctcc tcgacgactt cccgcgcttc gtcgctgccg
     3421 gccgcagtgc cgccgccgat cagcgccgac agccgtgcca gctggatctt ggtgtacgtg
     3481 agcgactgcg cgatcgaatc atgcagctcg cgcgcgatcg ccgagcgctc ctcgaagagc
     3541 gccagccgcc ggtgctcgtc gcggccggcc tccgccgcca gcgccgcgcc gatgtggcga
     3601 ccgatcacct gcgccagttc cagttgcgcc gctgtcagtt ggctccctgc caccagagtc
     3661 agcggcatca cgccgaagct gcgctcgccg tccagcagcg ggatcgacgc gatgcgcgcc
     3721 ggcccgcgct cgctgtcggc gtcgtgccag acgatctgcg tgctgtcgtc gcagccctcg
     3781 cagcgcacga tgtagcaggt gcgccccgcc gtctcctctg cgtgcgcgag cgggaatccg
     3841 ccgcgcgtgc ccgcttcgcg cgcgcagatc accacgccct cgacgccgag cacgtgccgc
     3901 acgctctcgg ccacgcgctg cagcgcgtct cgccgcaacg gcgggtgcgc cagctcccgc
     3961 gtggtttcgt acagcagcgc gagcgagcga ttcttctgtt cgaggtcggc ggtcttcacc
     4021 gcgatctgcg attcgagaga accgtaaagc cgcccgagct cctcgaccat gtgattgaaa
     4081 tcggcgccga gctggcccag ttcgtccggc ccggtgagct cggcgcgcgc gccgaagtcg
     4141 ccgcggcgca ctgcctgcgc gcagcggacg aggtcgcgca ccggctggaa gacctgcagg
     4201 tcgagcagga acaccgcgct gatcaccagc accagtgtca cgaacaaggc ggcgccgagc
     4261 gcgacctgca ggctatgaat gcgcgattcc aggtcgctct ccagctgcag cacgaagcga
     4321 tcgatcgcgg ccacgaacgg atggatctgc gacagcagcg tcgcgcgtgc cgcctcgtcc
     4381 gtcgccgcgc gcagcgcgag cggcttgatc tcgctgcgcc attgctcggc gatgcgctgg
     4441 tgcgcgcgcg ccagttcgcc gtccttgccg ccgaccaatc cgggcagcgc cgccaccagt
     4501 tgcgggtgcg tcagtcgcgc ctcgaagccg tcgatctcgc cgcggatcag cttctcgcgc
     4561 tccgccggcg ccacgtccag cgcggcgaca cgtgtggcga gcaggtagga ctgcatgcgg
     4621 agcgacccgg cgacgttgat cgccgcgccc ttgccgccgg agcgctccgt gaacacggcg
     4681 gcagtgatga cgaccgcgat cgccagcaac gcgatgatgc cgaggaccgt gctgacccgc
     4741 agcagggtcg acctgttcca ggcattcatg ctgggcttgc catgaaagga gttactacca
     4801 atgcgatacg ggcggtcatc gccgcttacc tccaacggag tacgaacttg atatcggacg
     4861 aagtgatccg gaccgcattt tgacctgagt caccgatcgt tgcgacagca ggcgaatgct
     4921 gcgagctgtt gctgcatttg gaccgcgttt ccgcactttc gcactttgct cctgaacctg
     4981 ccatggccaa cgtcgccgtc tccctgccct ccgcctcgcc gatgtccgcg ctgcgctcca
     5041 gacagtggtc cgtgctgatc gccagcacac tcggcttcac cgtctgtttc gcggtctgga
     5101 tgatgttcgc cgtgatcggg gtgccgatca agaagacgct cgatctgaac gccacccagt
     5161 tcggcctgct gactgccatg ccggtgctga ccggatcgct gatccgcgtg ccgctcggca
     5221 tgtggaccga caagttcggc ggccgcatcg ttttcttcct gctgatgctc tccacggtcg
     5281 tgccgatctg gttgatcagc tacgcgaccg agttctggca gttcctcgtg ctggggctgt
     5341 tcgtcggcct cgcgggcggg tcgttctcgg tcggcacgcc gtacgtggcg cgctggttcg
     5401 agcgcaagca gcagggcttc gcgatgggcg tgttcggcgc cggcaactcg ggcgcggcgg
     5461 tgaccaagtt cgtcgcgccg gcgctgctgg tcgcctacgg ctggacgatg gtgccgcagg
     5521 tgtatgcggt gtcgatgctg atcatggcga ttgccttctg gttcttctcg taccacgatc
     5581 cgaagcacac gatcgagagc cacatcacct accggcggca actcaccgtg ttgtcggacg
     5641 cgcgcgtgtg gaagtactgc cagtactact cgatcgtgtt cggcggctac gtcgcactgt
     5701 cgctgtggat ggtgaactac tacgtcggtg agttcggcct cgacatccgc gtcgcggccc
     5761 tgctggcggc atgcttctcg ctgcccggtg gcgtgctgcg cgccattggc ggctggatga
     5821 gcgacaagtg gggcgcccac aaagtgacgt ggtgggtgat gtgggtgagc tggatctgtc
     5881 tcttcctgct gtcgtatccg gccaccagct acacggtgca gacgctcaac ggcccgatca
     5941 ctttcacgct cgcgcacaac atgtggacgt tcacgttcct gatggcggtg ctcggcgtcg
     6001 catgggcgtt cggcaaggcg tcggtgttca agtacatctc
//
LOCUS       DBNHBY010000389         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_767861, whole genome shotgun sequence.
ACCESSION   DBNHBY010000389 DBNHBY010000000
VERSION     DBNHBY010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_767861"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="AC16HB_09655"
     CDS             complement(<1..1088)
                     /locus_tag="AC16HB_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607093.1"
                     /translation="MKFTKVSAILPAFTLAASLIAATAFAQVKVGVISSSTGPTAFVG
                     IPQKNAVALLPKKAGDLAIEYIVYDDASDPTQSVQLAKKLLSENRIDALIGPSGSPNA
                     MGVLQFMAEAQTPMLAPVGTSAVVLPMDDKKRWVFKTHPNDDVISEGLIAAMTKRGVK
                     TIGFIGYNDPYGENWYKTFAPMAQKAGIAVVANERYARTDTSVTGQVAKLIGAKPEAI
                     LVAGVAAGAALPHMQLIDAGYKGAIYHTHGSASGAFAQIGGKKVEGALIVGPMLLVLD
                     EIADSVQTKKVALEFVSSYDKQFGAKPPIFGAGVYDAGLLLARAIPDAAKKGKPGTPE
                     FRAGLRDAIEATHDLVASQGVINLSAQD"
     gene            complement(1120..>2009)
                     /locus_tag="AC16HB_09660"
     CDS             complement(1120..>2009)
                     /locus_tag="AC16HB_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5607094.1"
                     /translation="QDFLRHVLVDDVDGVKVITLRRPEALNALHDEMTDEVLAVIRRF
                     ENDPAVRGFVITGFGARAFCAGADIGRFPSMLGDAAASAQYARDCSRLLVHLDTMRKP
                     VVAALNGMALGGGLELAIRCHGIVAARSTFLQFPEITLGIVPGIGAMVVPYRRWPQAA
                     ETFHGMLRRAERLAAARARELGVVDDLADDIGTLIERAVARVGTLAGRTTAIAATPVA
                     LPALAAIEPVAANGQTLSREVLTILDDAVRRAAAAPSLAEALEVGYRAFGASACTAAA
                     REGIQANREGRPPDFGRTG"
BASE COUNT          288 a          717 c          664 g          340 t
ORIGIN      
        1 tggtcctgcg ccgacaggtt gatcacgccc tgcgatgcga ccaggtcatg cgtggcctcg
       61 atcgcgtcgc gcagcccggc gcggaactcg ggcgtgccgg gcttgccctt cttcgccgcg
      121 tccgggatcg cgcgtgccag cagcaggccc gcgtcgtaga ccccggcgcc gaagatcggc
      181 ggcttcgcgc cgaactgctt gtcgtagctc gaaacgaatt cgagcgcgac cttcttggtc
      241 tgcacgctgt cggcgatctc gtccagcacc agcagcatcg gcccgacgat cagtgccccc
      301 tcgaccttct tgccgccgat ctgcgcaaac gcgcctgacg ccgagccatg cgtgtgatag
      361 atcgcgccct tgtagccggc gtcgatcaat tgcatgtgcg gcagcgccgc gcccgccgcc
      421 acgccggcca cgagaatcgc ttccggcttg gcgccgatca gcttcgcgac ctggccggtc
      481 acgctggtgt cggtgcgcgc gtagcgctcg ttggcgacga cggcgatgcc ggccttctgc
      541 gccatcggcg cgaaggtctt gtaccagttc tcgccgtagg gatcgttgta gccgatgaag
      601 ccgatcgtct tcacgccgcg cttggtcatc gccgcgatca ggccctcgct gatcacgtcg
      661 tcgttcgggt gcgtcttgaa cacccagcgc ttcttgtcgt ccatcggcag cacgactgcc
      721 gaggtgccga ccggcgccag catcggcgtc tgcgcttcgg ccatgaactg cagcacgccc
      781 attgcgttcg gcgaaccgct cgggccgatc agcgcatcga tccggttctc cgacagcagc
      841 ttcttggcga gttgcacgct ctgcgtcgga tcgctggcgt cgtcgtagac gatgtactcg
      901 atcgcgaggt cgccggcctt cttcggcagc agcgcgaccg cgttcttctg cgggatgccg
      961 acgaatgcgg tcggtccggt cgacgagctg atcacgccga ctttcacctg cgcgaacgcg
     1021 gttgcggcga tgagcgacgc cgcgagcgtg aacgccggca ggatcgcgga cactttcgtg
     1081 aacttcatct gtcttcctcc ttcgctggtg tacgaacctc tatccggtcc tgccgaaatc
     1141 gggcgggcgg ccttcccgat tggcctggat gccttcgcgc gccgcggccg tgcaggcgct
     1201 cgcgccgaac gcgcggtagc cgacctcgag ggcttccgcc agcgacggcg cggcggccgc
     1261 ccggcgcacg gcgtcgtcga gaatcgtcag cacctcgcgg ctcagtgtct ggccattggc
     1321 tgcgaccggt tcgatcgcgg caagcgcagg gagcgcgacc ggcgtcgcgg cgatcgcggt
     1381 cgtgcgaccc gccagcgtgc ccacgcgcgc gaccgcgcgc tcgatcagcg taccgatgtc
     1441 gtccgcgaga tcatcgacga cgccgagttc acgcgcgcgc gccgccgcca atcgctcggc
     1501 gcggcggagc atgccgtgga acgtctcggc cgcctgcggc cagcggcgat acggcaccac
     1561 catcgcgccg ataccgggca cgatgccaag cgtgatctcg gggaactgca ggaacgtgct
     1621 gcgcgccgcg acgattccgt gacagcggat cgccagctcc agcccgccgc cgagcgccat
     1681 gccgttcagc gccgcgacca ccggcttcct catcgtgtcg aggtgtacca gcagccgcga
     1741 acagtcgcgc gcgtactggg cggacgcggc cgcgtcgcca agcatcgatg ggaaacggcc
     1801 gatgtcggcg ccggcgcaga acgcgcgcgc gccgaagccg gtgatcacga agccgcgcac
     1861 cgccggatcg ttctcgaagc gccggatgac cgccagcacc tcgtcggtca tttcgtcgtg
     1921 cagcgcgttc aatgcctcgg gtcggcgcag cgtgatcacc ttcacgccgt cgacgtcgtc
     1981 caccagcacg tggcgcagga agtcctgat
//
LOCUS       DBNHBY010000390         6299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1579115, whole genome shotgun sequence.
ACCESSION   DBNHBY010000390 DBNHBY010000000
VERSION     DBNHBY010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1579115"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16HB_09665"
     CDS             <1..506
                     /locus_tag="AC16HB_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HIR5607095.1"
                     /translation="KAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPKND
                     AFIKMYKDWAAKQKLPNADKVVTNDPMEATYIGIHMWKQGVEKAKTTEIDPVIKAVEG
                     QTVDAPCGFTVKMDEKNHHLHKPVFIGEVRADGQFNVVWKTKGPIRAQPWSPFIAGNE
                     GKIDSPA"
     gene            572..2182
                     /gene="urtB"
                     /locus_tag="AC16HB_09670"
     CDS             572..2182
                     /gene="urtB"
                     /locus_tag="AC16HB_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HIR5607096.1"
                     /translation="MRILVSALFGALLCLALPARAALDPALLVRLGADDSDLKIEAIR
                     KLIATEDPRAIEVLKAFGEDALALVDGGRRAVILADGRMLDAATGAALGAAPATVEAI
                     VINNRIRGEIGAALAALRLASPDRASRLAAAKELQGADNEDALPIIERATARELDAEI
                     KSILQLAGAALALKSGDPEQRIAAAKTLAASTDAQVRQMLATVVTRQKDGSYPESDAR
                     VRAAAQASLDDINRRLRTTEIGMALFSGASLGSILLLAALGLAITYGLMGIINMAHGE
                     FLMLGAYATYVVQGLFRQFAPGAFDWYVIAALPIAFAVTAAVGMALERSVLRWLYGRP
                     LETLLTTWGISLLLIQASRTVFGAQNVEVENPQWLSGGIALAGGYVLPFNRIAIIGFA
                     LVVLAAIWLVINRTRLGLFVRGVTQNRAMASCTGVPTQRVDMLTFGLGSGVAGLGGVA
                     LSQIGNVGPDLGQSYIVDSFMVVVLGGVGQLAGTVIAAFGLGSISKFLEPWSGAVMAK
                     IMVLAFIILFIQRRPQGLFALRGRSAEA"
     gene            2209..3300
                     /gene="urtC"
                     /locus_tag="AC16HB_09675"
     CDS             2209..3300
                     /gene="urtC"
                     /locus_tag="AC16HB_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HIR5607097.1"
                     /translation="MATLVAATVLLLIVPLLHLALPEQHALHLSSFWITLLGKFMCYA
                     VLALAMDLIWGYTGILSLGQGLFFALGGYAFGMSLMRQIGRDGVYKSELPDFMVFLDW
                     KALPWYWSGADSFLWCALLVVLVPGLVAFVFGFFAFRSRIKGVYFSIITQALTFAAML
                     AFFRNDMGFGGNNGFTDFKRILGFPITAPQTRTALYVLSCLYLLGALLVARWIVASKL
                     GRVLTAIRDQESRLMFLGYRTLYFKLAIWTLAAIMSGIAGALYVPQVGIINPSEMSPA
                     NSIEIAIWAAVGGRGTLFGPVLGAFAVNGAKSWFTVAYPEYWLYFLGLLFVLATLFLP
                     RGLIGLFGKLRLRRAEADPTATAAVQARP"
     gene            3297..4118
                     /gene="urtD"
                     /locus_tag="AC16HB_09680"
     CDS             3297..4118
                     /gene="urtD"
                     /locus_tag="AC16HB_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HIR5607098.1"
                     /translation="MSPAIHGIGSSGGVGYGHPINANRVDTSHGPILYLEDVTVSFDG
                     FKALNKLSMTIDRGELRCVIGPNGAGKTTMMDVITGKTRPDAGTAFFGQTFDLARLPE
                     TEIARLGIGRKFQKPTVFESHTVFENLELAMKCDKRVHPTLLAKLTGAQRDRIARTLR
                     TIRLEDAAQRRAGSLSHGQKQWLEIGMLLVQEPELILLDEPVAGMTDAETDRTAELIL
                     ELEGSHSIVVVEHDMDFVARIARKVTVLHEGSVLAEGGMQQVQGNERVIEVYLGR"
     gene            4121..4819
                     /gene="urtE"
                     /locus_tag="AC16HB_09685"
     CDS             4121..4819
                     /gene="urtE"
                     /locus_tag="AC16HB_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HIR5607099.1"
                     /translation="MLTIDGLNQYYGGSHILRDVTLAVATGACTVLLGRNGVGKTTLL
                     KTAMGLVPARSGTIRLDGRDITGLAPYQRAALGIGYVPQGREIFNRLTVEENLLMGLA
                     PVRGRAARKVPDAIFETFPVLARMLKRRGGDLSGGQQQQLAIGRALAANPCVLILDEP
                     TEGIQPSIIKEIGAVLRALVERSKMAVLLVEQYYDFAESLADRYAVMSRGQITHAGAG
                     REMRERNVRALVAI"
     gene            4831..5637
                     /locus_tag="AC16HB_09690"
     CDS             4831..5637
                     /locus_tag="AC16HB_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HIR5607100.1"
                     /translation="MQTWQASLALDYRRSARRTVVSARHAGPLRVQKALYPEGDAVCH
                     TVIVHPPGGIAGGDALQIAVDAQPGSHALITTPGAAKWYKANGRPASQRVHLQVDGAL
                     EWLPQEAIVFDQADAGSRIDIDVGPDAAMIGWDIVVLGRLAAGESFRAGCFAQTIRLR
                     DDGALQWVERMRLAGGDALLASPIGLAGNHVFGCLWAIGPRWTDAQIESLRDRLPDES
                     PVTRLAPRLLVARALGKASAPVRRALEAVWRELRPLAFERSATPPRIWAT"
     gene            5655..5957
                     /locus_tag="AC16HB_09695"
     CDS             5655..5957
                     /locus_tag="AC16HB_09695"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIR5607101.1"
                     /translation="MELTPREKDKLLIFTAALLAERRRSRGLKLNYPEAVAFVTAAIM
                     EGARDGRTVADLMHYGTTLLTRTDVMDGVPEMIPDIQVEATFPDGTKLVTVHNPIA"
     gene            5964..>6299
                     /locus_tag="AC16HB_09700"
     CDS             5964..>6299
                     /locus_tag="AC16HB_09700"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIR5607102.1"
                     /translation="MIPGELLVAAGDIELNAGRRTLRLTVANTGDRPIQVGSHYHFFE
                     TNEALRFDRAAARGFRLNIPAGTAVRFEPGQEREVELVALGGDRIVYGFNAKVMGPLD
                     ARKPAQKAAK"
BASE COUNT         1020 a         2060 c         2207 g         1012 t
ORIGIN      
        1 tgaaagcgaa ggacgtgccg gtggtcgcgt tctcggtcgg cgaggaggaa ctgcgcggcg
       61 tcgacaccaa gccgctggtg ggccatctgg cggcgtggaa ctacttcatg tcgctgaaga
      121 atccgaagaa cgacgcgttc atcaagatgt acaaggactg ggcggccaag caaaagctgc
      181 cgaacgccga caaggtcgtc accaacgatc cgatggaggc cacctacatc ggcatccaca
      241 tgtggaagca gggcgtggag aaggcgaaga ccaccgagat cgatccggtg atcaaggcgg
      301 tcgaaggcca gacggtcgac gcgccgtgcg ggttcacggt gaagatggac gagaagaacc
      361 atcacctgca caagccggtg ttcatcggcg aggtgcgcgc cgatggccag ttcaatgtcg
      421 tgtggaagac caaaggcccg atccgcgccc agccctggag cccgttcatc gccggcaacg
      481 aaggcaagat cgattcgccg gcctgagcgg ccgatctgcg cgccgccgac cgtgaagaca
      541 cggcggccgg cgccgcagcg ggagacacgc catgcggatt ctggtgagcg cgctcttcgg
      601 cgcgctgctg tgcctcgccc tgccggcccg ggccgcgctc gacccggcgc tgctggtccg
      661 gctgggcgcc gacgactcgg atctcaagat cgaggcgatc cgcaagctca tcgcgaccga
      721 ggatccacgc gcgatcgagg tgctgaaggc gttcggtgaa gatgcgctcg cgctggtgga
      781 tggcggcagg cgcgcggtga tcctcgctga cggccggatg ctggacgcgg cgaccggcgc
      841 cgcgctcggc gcggcgcccg cgacagtgga agcgatcgtc atcaacaacc ggatccgcgg
      901 cgagatcggc gccgcgctgg cggcactgcg gctcgccagc ccggatcgcg cgtcgaggtt
      961 ggccgcggcg aaggaactgc agggagcgga caacgaggac gcgctgccga tcatcgagcg
     1021 cgcaaccgcg cgcgaactgg acgccgagat caagtcgatc ctgcaactcg ccggcgccgc
     1081 gctggcactg aagagcggcg atcccgagca acgcatcgcc gccgcgaaga cgctcgccgc
     1141 gagcaccgat gcgcaggtgc ggcagatgct ggcgaccgtc gtcacccgac agaaggacgg
     1201 ctcgtatccg gaatccgacg cgcgcgtgcg cgccgctgcg caggcgtcgc tcgacgacat
     1261 caaccgccgc ctgcgcacga ccgagatcgg gatggcactg ttttccggcg ccagcctcgg
     1321 ctcgatcctg ctgctcgccg cactcggact cgcgatcacc tacggcctga tgggcatcat
     1381 caacatggcg cacggggagt tcctcatgct cggtgcgtac gcgacctatg tcgtgcaagg
     1441 actgttccgc cagttcgcgc cgggcgcgtt cgactggtac gtgatcgcgg cgctgccgat
     1501 cgcatttgcg gtgacagccg cagtcggcat ggcgctcgag cgcagcgtgc tgcgctggct
     1561 gtacggccgc ccgctggaga cgctgctgac gacctggggc atcagcctgc tgctgatcca
     1621 ggcgagccgg acggtcttcg gcgcgcagaa cgtcgaagtg gagaacccgc agtggctgtc
     1681 gggcggaatc gcgctcgcgg gcggctacgt actgccgttc aatcgcattg cgatcatcgg
     1741 tttcgcgctc gtcgtgctcg ccgcgatctg gctcgtgatc aaccgcacgc ggctgggcct
     1801 gttcgtgcgc ggcgtcaccc agaaccgcgc gatggccagc tgcaccggcg tgccgacgca
     1861 gcgcgtcgac atgctgacgt tcgggcttgg atcgggcgtg gcgggattgg gcggcgtggc
     1921 gctgtcgcag atcggcaatg tcggaccgga tctcggacag agctacatcg tcgacagctt
     1981 catggtggtc gtgctcggcg gcgtcggcca gctcgccggc accgtgatcg ccgccttcgg
     2041 cctcgggtcg atttccaagt tcctcgaacc gtggtcgggc gcggtgatgg ccaagatcat
     2101 ggtgctggcg ttcatcatcc tcttcattca gcggcgaccg cagggcctgt tcgcgctgag
     2161 gggacggagt gcggaggcat gacgcgcaac tttccgcccc gcctctggat ggcgaccctg
     2221 gtcgccgcaa ccgtactgct gctcatcgtt ccgctgctgc acctcgcgct gccggaacag
     2281 cacgcgctgc acctgtcgag cttctggatc acgctgctcg gcaagttcat gtgctacgcg
     2341 gtgctcgcgc tggcgatgga cctgatctgg ggctacacgg ggatcctgtc gctcgggcag
     2401 gggctgttct tcgctctcgg aggctatgcg ttcggcatgt ccctgatgcg gcagatcggg
     2461 cgcgacggcg tctacaagag cgagttgccc gacttcatgg tgttcctcga ctggaaggcg
     2521 ctgccttggt actggagcgg agccgactcg ttcctctggt gcgcgttgct ggtcgtgctc
     2581 gttccaggac tggttgcgtt cgtgttcgga ttcttcgcgt tccggtcgcg catcaagggc
     2641 gtgtatttct cgatcatcac gcaggcgctg acgttcgcgg cgatgctggc cttcttccgc
     2701 aatgacatgg ggttcggcgg caacaacggc ttcaccgact tcaagcgcat cctcggattt
     2761 ccgatcacgg cgccgcagac ccgcaccgcg ctgtacgtgc tgtcgtgcct gtatctgctc
     2821 ggcgcgctgc tggtggcacg ctggatcgtc gcgagcaagc tcggccgcgt gctgaccgca
     2881 atccgcgacc aggagtcgcg gctgatgttt ctcggttacc ggacgctgta tttcaagctc
     2941 gcgatctgga cactcgccgc gatcatgagc ggcatcgccg gcgcgctgta cgtgccgcag
     3001 gtcggcatca tcaacccgag cgagatgagc cccgccaact cgatcgagat cgcgatctgg
     3061 gcggcggtcg gcgggcgtgg cacgctgttc gggccggtcc tgggcgcgtt cgcggtcaat
     3121 ggtgcgaaga gctggttcac ggtcgcgtac ccggagtact ggctgtactt cctcggcctg
     3181 ctgttcgtgc tggcgacgct gttcctgccg cgcggcctga tcggtctctt cgggaagttg
     3241 cgcttgcgtc gcgcggaagc cgacccgacc gcgaccgccg cggtgcaggc gcgcccatga
     3301 gcccggcgat ccacggtatc ggctcctcgg gcggcgtcgg ctacgggcac ccgattaacg
     3361 ccaaccgcgt cgacacgtcg catggaccga tcctgtatct ggaagacgtc acggtcagct
     3421 tcgacggctt caaggcgctg aacaaactga gcatgacgat cgatcgcggc gagttgcgct
     3481 gcgtgatcgg acccaatggc gccggcaaga ccacgatgat ggacgtgatc accggcaaga
     3541 cgcggcccga cgccggcacc gcgttcttcg gccagacctt cgacctggcg cgcctgccgg
     3601 agaccgagat cgcgcgcctg gggatcggac gcaagttcca gaagcccacc gtgttcgaga
     3661 gccacacggt gttcgagaac ctcgaactgg cgatgaagtg cgacaagcgc gtgcacccga
     3721 cgctgctcgc gaagctcacc ggtgcgcagc gcgaccggat cgcgcgcacg ctgcggacaa
     3781 tccggctcga ggacgcggcg cagcgccgcg ccggatcgct gtcgcacggg cagaagcagt
     3841 ggctcgaaat cggcatgctg ctggtgcagg agccggagct gatcctgctc gacgagcccg
     3901 tggccggcat gaccgatgcc gagacggacc gcaccgccga actgatcctc gagctcgaag
     3961 gcagccactc gatcgtcgtg gtagagcacg acatggattt cgtggcacgc atcgcgcgca
     4021 aggtgaccgt gctgcacgaa ggcagcgtgc tcgccgaagg cggcatgcag caggtgcagg
     4081 gcaacgagcg agtgatcgag gtctatctgg ggcggtgacg gtgctgacga tcgacggact
     4141 gaaccagtac tacggcggca gccacatcct gcgggacgtg accttggccg tggccaccgg
     4201 cgcgtgcacg gtgctgctgg gccgcaatgg tgtcggcaag accacgctgc tgaagacggc
     4261 gatggggctc gtaccggcgc gcagcggaac catccgcctc gacgggcgcg acatcacggg
     4321 gctggccccc taccagcgcg ccgcgctcgg catcgggtac gtgccgcagg gacgcgagat
     4381 cttcaatcgg ctcacggtcg aggagaacct gctgatgggc ctggcgccgg tccgcgggcg
     4441 cgcggcgcgc aaggtgccgg acgcaatctt cgagacgttt ccggtgctcg caaggatgct
     4501 caagcgccgc ggcggcgatc tgtcgggcgg ccagcagcag cagctcgcga tcgggcgcgc
     4561 actcgcggcg aacccgtgcg tgctgatcct cgacgagccg accgaaggca ttcagccttc
     4621 gatcatcaag gagatcggcg ccgtgctgcg cgcgctggtg gaacgtagca aaatggccgt
     4681 gctgctcgtg gagcagtact acgacttcgc cgaatcgctc gcggaccgtt atgcggtgat
     4741 gtcgcgcggc cagatcaccc atgcgggcgc gggccgcgag atgcgggaaa ggaatgttcg
     4801 cgcgctggtc gccatctgag gcggaacttc ttgcaaacct ggcaagcgtc cctcgcgctg
     4861 gactaccgcc gctccgcgcg gcgcaccgtg gtgtcggcgc ggcatgcggg ccctttgcgc
     4921 gtgcagaagg cgctctatcc cgagggtgac gcggtgtgcc acaccgtcat cgtgcatccg
     4981 ccgggcggga tcgccggcgg cgatgcgctg cagatcgccg tcgacgcgca acccggctcg
     5041 cacgcgctga tcaccacacc cggcgcggcc aagtggtaca aggccaacgg ccggccagcc
     5101 tcgcagcgcg tccacctgca ggtggacggc gcgctcgagt ggctgccgca ggaggcgatc
     5161 gtgttcgatc aggcggacgc cggctcgcgg atcgacatcg atgtcggacc cgatgcggcg
     5221 atgatcggct gggacatcgt ggtgctcggt cgcctcgctg ctggcgaatc gttccgcgcg
     5281 ggctgctttg cgcagacgat ccgcctgaga gatgacggcg cgctgcaatg ggtcgagcgc
     5341 atgcgtctgg caggtggcga cgcattgctc gcctcgccga tcgggctggc cggcaaccac
     5401 gtgttcggct gcctgtgggc gatcggcccg cgctggaccg acgcgcagat cgaatcgctg
     5461 cgcgaccgcc tgcccgacga gtcgccggta acgcggctgg cgccgcggct gctcgtcgcg
     5521 cgcgcgctcg gaaaggcctc ggccccggtc cggcgtgcgc tggaggcggt gtggcgcgaa
     5581 ctgcgcccgc tcgcattcga gcgatccgcg acgccgccac gcatctgggc gacctgaccg
     5641 gcgaggatca cgcgatggaa ctgactccga gggagaagga caagctcctg atcttcaccg
     5701 ccgcgctgct ggccgagcgg cggcgctcgc gcgggctgaa gctcaactat cccgaggcgg
     5761 tcgctttcgt caccgccgca atcatggaag gcgcgcgcga cggccgcaca gtcgcggacc
     5821 tgatgcatta cggcacgacg ctgctgacac gcaccgacgt gatggacggc gtgccggaga
     5881 tgatccccga catccaggtc gaagcgacgt ttccggacgg caccaagctc gtcacggtgc
     5941 acaacccgat tgcgtgaggc gccatgatcc ccggagaact gctcgtcgcc gccggcgaca
     6001 tcgaattgaa cgccggccgc aggacgctgc ggctcacggt ggcgaacacc ggcgaccggc
     6061 cgatccaggt cggctcgcac taccacttct tcgagaccaa cgaggcgctg cgcttcgacc
     6121 gcgcggccgc acgcggcttt cggctgaaca tcccggcggg cacggccgtg cgcttcgagc
     6181 ctgggcagga acgcgaggtg gaactcgtag cgctcggcgg cgaccggatc gtttacggct
     6241 tcaacgcgaa ggtcatgggg ccactggacg ccaggaagcc ggcgcagaag gcggcgaag
//
LOCUS       DBNHBY010000391         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_90462, whole genome shotgun sequence.
ACCESSION   DBNHBY010000391 DBNHBY010000000
VERSION     DBNHBY010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_90462"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="AC16HB_09705"
     CDS             <1..637
                     /locus_tag="AC16HB_09705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR5607103.1"
                     /translation="AAPNGNLYVVWQDGRFVNGIDSIAFARSTDGGLTWSAPVRINGV
                     ANAQAFTPQVAVLADGTIGVTYFDLRSNTPDLNTLPTEQWLTRSRDGGSTWSEVRVAG
                     PFDLAIAPNARGLFLGDYQGLAASGGRFVSLFAQTTRAALANPTDIYALAITPAAQAQ
                     TATPSARAYRAQAVAGEPTDAMRARASENMLRVLERRVPGWTDRMTAPPAR"
     gene            complement(834..1220)
                     /locus_tag="AC16HB_09710"
     CDS             complement(834..1220)
                     /locus_tag="AC16HB_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5607104.1"
                     /translation="MDVYKTHGAFSWSELLTSDPAAARDFYTKLFGWSSKEMPMPNGA
                     YTTFQVGDTSVGGMMMIPADAAGMPPMWGVYVTVKDVDATAKEAEALGGKMLMPPADI
                     PGVGRWALIQDPQGATLSLITYSMPG"
     gene            1402..1716
                     /locus_tag="AC16HB_09715"
     CDS             1402..1716
                     /locus_tag="AC16HB_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607105.1"
                     /translation="MQTTGDRFVDLSLRILTLVVGAVLLACLGLLLYRWSAPAAIAPV
                     VTIVPVTPKLPLPTTAAAPIADAERAPEVMMAPGKMFQCVVNGRKLFSDRPCPEAAGT
                     RK"
     gene            1828..2166
                     /locus_tag="AC16HB_09720"
     CDS             1828..2166
                     /locus_tag="AC16HB_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607106.1"
                     /translation="MTKRFTLLTAAALIAVALPLGAAQAQQVSVRIETPDIGVRIGTP
                     YPPPVYAPVYAPPPVLVAPAPVYYSAPRYVIAPTPRVFVPAPVVYRSPWYGPRAYRYK
                     HWKRYRDWND"
BASE COUNT          324 a          783 c          699 g          365 t
ORIGIN      
        1 cgcggcgcca aacggcaacc tgtatgtcgt ctggcaggac ggccgcttcg tcaacggcat
       61 cgacagcatc gcgttcgcac gctcgaccga cggcgggctc acgtggtcgg cgccggtgcg
      121 catcaacggc gtcgcgaacg cgcaggcatt cacgcctcag gtcgccgtgc tggccgacgg
      181 cacgatcggc gtcacttact tcgacctgcg cagcaatacg cccgacctga acacgctgcc
      241 caccgagcaa tggctgacgc gctcgcgcga tggcggcagc acgtggagcg aggtccgcgt
      301 ggctggcccg ttcgacctgg cgatcgcacc gaacgcgcgc ggcctgttcc tcggcgatta
      361 ccagggcctt gcggcaagtg gcggccgctt cgtgtcgttg ttcgcacaga caacgcgcgc
      421 ggcactggcc aatccgaccg acatctacgc gctggccatc accccggcgg cgcaggcgca
      481 gaccgccacg ccgtcggcac gcgcgtatcg cgcgcaggcg gtggccggcg agcccaccga
      541 cgcgatgcgc gcgcgcgcca gcgagaacat gctgcgcgtg ctcgagcgtc gcgtgcccgg
      601 atggaccgat cggatgacgg cgccgcccgc gcgttgagtc gcgtgcccga cgcgcgccgc
      661 gaccgtcggc cgcaggcgcc cgtgcatcac gcgccgctgg cgatcgcagc gatgatgggg
      721 gaccctcgag acactgtttc gatggacgat ttcatcgaac cggttgtctc ctccacctcc
      781 tcctgtggtg gttcgggccc gacggcagtg tctcctcggg cccgtttctt ttttcaaccc
      841 ggcatcgaat acgtgatcag cgacagcgtt gcgccctgcg gatcctggat caacgcccag
      901 cggccgacgc cgggaatgtc cgcggggggc atcagcatct tgccgcccag cgcttcggcc
      961 tctttcgccg ttgcgtcgac gtccttgacg gtcacgtaca cgccccacat cggtggcatg
     1021 ccggcggcat cggcgggaat catcatcatg ccgccgaccg acgtgtcgcc gacctggaac
     1081 gtcgtgtagg cgccgttcgg catcggcatc tccttgctgc tccagccgaa cagcttcgtg
     1141 tagaagtcgc gcgcggccgc cggatcgctg gtcagcagtt ctgaccagct gaatgcaccg
     1201 tgcgtcttgt aaacgtccat gccgtcctcc tttccgtctg cgggcggtca tgatcgcgcg
     1261 ccccggagcg cgccgccagc gcgcaagcgc gtaggcgccg gccgctccca ggtgcaaccg
     1321 cgcgaatgcg cacgtcgacg agatgccatc tgcgcgataa tcgcgcttcg ttttcacccg
     1381 ccacggccga tccgcacgcg catgcagacc acaggagatc gcttcgtcga tctgtcgttg
     1441 cgcatcctca cgctcgtggt cggcgccgtg ctgctcgcgt gcctggggct gctgctgtat
     1501 cggtggtcgg cacccgctgc aatcgcgccg gtcgtgacga tcgtgccggt cacgccgaaa
     1561 ttgcctctgc cgacgactgc cgccgcgccg atcgccgacg ccgaacgcgc gccggaagtc
     1621 atgatggcgc caggcaagat gtttcagtgc gtggtcaacg ggcgcaagct gttctccgac
     1681 cggccctgtc ccgaagcggc gggcacccgg aagtagcctt atcccggtaa caactggcaa
     1741 aacacccgcg caccgcgcgg tcggcgcgat cgcggcgcgg acgttgttcg aggcatggtg
     1801 cagtcgaatc catccctcgg agcagccatg acgaaacgat tcaccctcct caccgccgcc
     1861 gcgctgatcg cggtcgcact gccccttggc gcagcgcagg cgcagcaggt ttcggtccgc
     1921 atcgaaacgc ccgacatcgg cgtgcgcatc ggtacgccgt atccgccacc cgtgtacgcg
     1981 ccggtgtatg cgccgccacc ggtgttggtc gcgcccgcac ctgtctacta ctcggcgccg
     2041 cgctacgtca tcgcgccgac gccgcgcgtg ttcgtgccgg cgccggtcgt ctatcgctcg
     2101 ccctggtacg gcccacgcgc gtaccggtac aagcactgga agcgctaccg cgactggaac
     2161 gattgatttg a
//
LOCUS       DBNHBY010000392         5454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_90614, whole genome shotgun sequence.
ACCESSION   DBNHBY010000392 DBNHBY010000000
VERSION     DBNHBY010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_90614"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16HB_09725"
     CDS             <1..275
                     /locus_tag="AC16HB_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607107.1"
                     /translation="VLIPGAAVAAAAALLVVVAMPQLRGAGEAPRIAATTPAAAPAST
                     PQPVEVARVPELERYLRAHREMAGSLVMPDATPYLRTSNNLPGETR"
     gene            275..1330
                     /locus_tag="AC16HB_09730"
     CDS             275..1330
                     /locus_tag="AC16HB_09730"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR5607108.1"
                     /translation="MAGGRAPRPTRTPDAILHQRILLALMLALLAYVGSAPAQSNPPP
                     KAAPRGDAQRTELQWLQAIQAAAQRLTYAGTVIYQQGEEVRASRLIHVFDGNVAQERL
                     QPLDGQLREFIRRADEVQCLMPESRRIVVERRVAQDAFPAIATADPAEILEHYTVRIG
                     GVERVAGVECQVIQIEPKDRLRYGYRLWVDRTTGLLLRAVTLNERSEVLEQMAFAEVR
                     TGDRVDRSQLKPSWPIEGWRVERTEQPPVDLAQKGWHISPPAGFRKLREVMRAPWGAN
                     VERKVMQSVLTDGIANVSVFIEPAAQESTVEAAQSNGPTSVYARRVGDARVTVVGEVP
                     MATVKALAKSVEFRAPR"
     gene            1355..2791
                     /locus_tag="AC16HB_09735"
     CDS             1355..2791
                     /locus_tag="AC16HB_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR5607109.1"
                     /translation="MKLLTRFLAFSMFSLAAIQAPAQTAQLPEFTDLVERASPAVVNI
                     RTTARVGAGQAMPQIPGLEEGDPFYEFFRRFFPQPMPQPRQGPQPRTPRSDREVPRGL
                     GSGFVVSPDGYVMTNHHVVDGADEIIVRLADQREFRGKLIGSDQRTDVALVKIEATNL
                     PTVRIGDSSKLKVGEWVIAIGSPFGLDSTVTAGIVSAKARETGDFLPFIQTDAAVNPG
                     NSGGPLLNMRGEVVGINSQIFTTSGSYAGISFAIPIDEAMRVQEQLRAQGRVIRGRIG
                     VTIGEVTRDVAEGIGLSKPQGALVSSVERDGPADKSGVEPGDVILRFDGKAIDRSSEL
                     PRVVGGTKPGSRVAMSVWRKGKNVDLQVTVAELQTDTRQARRGEQTPKPVPATNPLGL
                     AVSDVPADKLKELRIKGAVQVDGAEGPAAAAGIRVGDLILSLNNVDIQNAKQFNEIAA
                     KLDLKRNVAALVRSGEQTRFVIIRPTNR"
     gene            2964..4757
                     /gene="lepA"
                     /locus_tag="AC16HB_09740"
     CDS             2964..4757
                     /gene="lepA"
                     /locus_tag="AC16HB_09740"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR5607110.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRLIQRCGGLSEREMEEQVLDSM
                     ALERERGITIKAQTASLEYEAADGTKYELNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIELGVEVLPVLNKMDLPQANPDEARAEIEDVIGIDAHDAVL
                     ASAKTGMGVDEILERIIARVPPPRGEPSAPLQALIIDSWFDNYVGVVTLVRVVNGAIR
                     PKDKVLLMATGAAHLVEQVGVFTPKSQPREELNAGEVGFVISGIKELRDARVGDTITH
                     AAKPAAAPLPGFKEIKPQVFAGLYPVEANQYEALRDALTKLQLNDASLHFEPEVSQAL
                     GFGFRCGFLGLLHMDIVQERLEREYDMDLITTAPSVVYEVLLRDGSVVQVENPSRLPD
                     PAKIEEIREPIVSVTIFVPQEYVGPVMTLCTGKRGVQVDLAYHSRHVHLTYELPLAEI
                     VLDFFDKLKSVSRGYASMDYEFREYRAADVVKVDMLINGDRVDALSVIVHRSSSQYRG
                     REIAAKMRALIPRQMYDVAIQAAIGANIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGTVEIPQEAFLAILQVEEK"
     gene            4754..>5454
                     /gene="lepB"
                     /locus_tag="AC16HB_09745"
     CDS             4754..>5454
                     /gene="lepB"
                     /locus_tag="AC16HB_09745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5607111.1"
                     /translation="MNFALILFILLVVSFAMWLLDLLWLKKTRFAAASAALAEFDARN
                     AGKIGVNTAALAADRSALEDRITRRPWYLEYTASFFPVILLVFVLRSFFFEPFRIPSG
                     SMIPTLQVGDLILVNKYQYGIRLPVINKKVIDIGSPQRGDVMVFRFPHNPSQDYIKRV
                     IGLPGDRVEYLDRRLTINGKPVETVGVGRYLEEGRLQYYAEDTEKLGQVSHRIILSES
                     PMGVPIMSTGQHTNP"
BASE COUNT          979 a         1760 c         1857 g          858 t
ORIGIN      
        1 gagtgctgat cccgggcgcc gcggtggcgg cggcggccgc gctgctggtc gtcgtcgcca
       61 tgccgcagtt gcgcggggct ggcgaggctc cacggattgc ggcgaccaca ccggccgccg
      121 cgccggccag cacgccgcag ccggtcgaag tcgcgcgcgt tcccgaactt gagcgctacc
      181 tgcgcgcgca tcgtgagatg gcgggcagcc tggtgatgcc cgacgccacg ccgtatctgc
      241 gcacgtccaa caacctgcct ggggagacgc gttgatggca ggcggccggg ccccgcgccc
      301 gacccggacg ccggacgcca tactccacca gcgcatcctg ctcgcgctga tgctggcact
      361 gctcgcatac gtcggcagcg cgccggcgca atcgaatccg ccgcccaagg cggctccgcg
      421 cggcgatgcg cagcgcaccg aactgcagtg gctgcaggcg atccaggcgg cggcgcagcg
      481 cctgacgtat gccggcacgg tcatctacca gcagggcgag gaggtccgcg cgtcgcggct
      541 catccacgtg ttcgacggca acgtcgcgca ggagcggttg cagccactgg acgggcagtt
      601 gcgcgagttc atccggcgcg ccgacgaagt gcagtgcctg atgccggaat cgcgccgcat
      661 cgtcgtcgag cgccgtgtcg cccaggacgc gtttcccgcg atcgccaccg cggatcccgc
      721 cgagatcctc gaacattaca cggtgcggat cggcggcgtg gagcgcgtcg ccggcgtcga
      781 gtgccaggtg atccagatcg aaccgaaaga ccggctgcgc tacggctacc gcctgtgggt
      841 cgatcgcacg accgggctgc tgctgcgcgc tgtaactctg aacgagcgca gcgaagtgct
      901 ggagcagatg gcattcgccg aggtgcgaac cggggatcgc gtcgatcgca gccagctgaa
      961 gccgtcgtgg ccgatcgagg gctggcgcgt cgagcgcacc gagcagccgc cggtcgacct
     1021 ggcgcagaag ggctggcaca tctctccgcc cgcgggtttt cgcaagcttc gcgaggtgat
     1081 gcgggcgccg tggggcgcca acgtcgagcg caaagtgatg cagtcggtgc tgacggacgg
     1141 aatcgccaat gtgtcggtgt tcatcgaacc tgcagcacag gaatcgacgg tcgaggccgc
     1201 gcagtccaac ggcccgacca gcgtgtacgc gcgccgtgtg ggggacgcgc gcgtgacggt
     1261 cgtcggcgaa gtgccgatgg cgaccgtgaa ggcattggcg aaatcagtcg agtttcgcgc
     1321 gccccgctaa gcaggccaac aattcgagac gcagatgaaa ttgctgacga gattccttgc
     1381 cttttccatg ttttcgctcg cggcgatcca ggcgccggcg cagacggcgc agttgccgga
     1441 gttcacggat ctggtcgagc gcgccagccc ggccgtggtc aacattcgca ccacggcccg
     1501 cgtcggcgct ggccaggcca tgccgcagat tcccgggctg gaagagggcg acccgttcta
     1561 cgagttcttc cgccgtttct ttccgcagcc gatgccgcag ccgaggcagg gcccgcagcc
     1621 gcgcacgccg cgcagcgacc gtgaggtgcc gcgcgggctg ggctccggct tcgtcgtttc
     1681 gcccgacggc tacgtgatga ccaaccatca cgtggtggac ggcgccgacg agatcatcgt
     1741 ccgactggcc gaccaacgcg aattccgcgg caagctgatc ggctccgatc agcgcaccga
     1801 cgtggcgttg gtgaagatcg aggccacgaa tctgccgacc gttcgcatcg gcgattcctc
     1861 gaagctgaag gtcggcgaat gggtgattgc gatcggttcg ccgttcggcc tcgacagcac
     1921 ggtgaccgcc ggcatcgtca gcgccaaggc gcgcgagacg ggggacttcc tgcccttcat
     1981 ccagaccgac gcagcggtca acccgggcaa ttcgggcggg ccgctgctca acatgcgcgg
     2041 cgaggttgtc gggatcaact cacagatctt cacgacctcc ggctcgtacg ccggaatctc
     2101 gttcgcgatt ccgatcgacg aggcgatgcg ggtgcaggaa cagctgcgcg cgcagggccg
     2161 cgtgatccgc ggccgcatcg gcgtcacgat cggcgaagtc acgcgcgacg tggcggaggg
     2221 aatcggcctt tcgaagccgc agggcgcgct ggtatcgagc gtggagcgcg acggtccggc
     2281 cgacaagtca ggcgtggaac cgggcgacgt gatcctgcgc ttcgacggca aggcgatcga
     2341 ccggtcgtcc gaactgccgc gcgtggtcgg cggcaccaag ccggggtcgc gcgtcgcgat
     2401 gtcggtctgg cgcaagggca agaacgtcga ccttcaggtc acggtggccg aactgcaaac
     2461 cgacacgcgt caagcgcgcc gcggtgagca gacgcccaag ccggtgccgg ccacgaatcc
     2521 gcttggactg gcggtttccg atgtgccggc cgacaaactg aaggaactgc ggatcaaggg
     2581 cgcagtacag gtcgatggtg ccgaagggcc ggcggccgcg gctgggatcc gggtcggcga
     2641 cctgatcctg tcgctcaata atgtcgacat ccagaacgcc aagcaattca atgaaatagc
     2701 cgctaaactt gatctgaaac gtaacgtcgc tgcgctggtc cgttccggcg agcagacgcg
     2761 attcgtgatc attcgtccca ccaatcggta ggcgcggaca gcggccggga gcagtcgagc
     2821 acgggcgcta gaatgcgcgc ccaatcaagg ggtggtccgg tgggtcaccc ctttttatcg
     2881 ccgtgccggc aagagcatga atcgcgatcc ggccgtggcc gcaacgggta tcgcggcgca
     2941 gtcggcgctc catcccaggc gcaatgcaac acatacgcaa cttttccatc atcgcccaca
     3001 tcgaccacgg caagtcgacg ctggcggatc ggctgatcca gcgctgcggc ggcttgtccg
     3061 agcgcgagat ggaggagcag gtactcgact cgatggcgct cgagcgtgag cgaggaataa
     3121 ccatcaaggc gcagaccgca tcgctcgagt acgaggccgc agacgggacg aagtacgaac
     3181 tgaacctgat cgacacgcca ggccatgtcg acttctcgta cgaggtcagc cgctcgctgt
     3241 ctgcctgcga aggcgcgctg ctggtggtgg acgcgtcgca gggcgtcgaa gcccagacgg
     3301 tcgccaattg ctacaccgcg atcgaactcg gcgtcgaagt gctgccggtg ctgaacaaga
     3361 tggacctgcc ccaggccaat ccggacgagg cgcgcgccga gatcgaggac gtgatcggca
     3421 tcgacgccca cgacgcggtg cttgccagcg ccaagaccgg catgggagtg gatgagatcc
     3481 tcgagcgcat catcgcgcgc gtgccgccgc ccaggggcga accgtccgcg ccgctgcagg
     3541 ccctgatcat cgactcctgg ttcgacaact acgtcggcgt ggtgacgctg gtgcgggtgg
     3601 tcaacggcgc catccgcccg aaggacaagg ttctgctgat ggccaccggc gccgcacacc
     3661 tggtcgagca ggtcggggtg tttacgccga agtcccagcc gcgggaggag ctgaacgccg
     3721 gagaggtcgg cttcgtgatc tccggcatca aggaactgcg cgacgcgcgc gtcggcgaca
     3781 cgatcacgca cgccgcgaag ccggccgccg cgccgctgcc cggattcaag gagatcaagc
     3841 cgcaggtgtt cgctggcctg tatccggtcg aggcgaacca gtacgaggcg ctgcgcgacg
     3901 ccttgacgaa gctgcagctg aacgacgcgt cgctgcactt cgagcccgaa gtgtcacagg
     3961 cactgggctt cgggttccgc tgtggcttcc tgggtctgct ccacatggac atcgtgcagg
     4021 agcggctcga gcgcgagtac gacatggacc tgatcaccac cgcgccgtcg gtggtctacg
     4081 aggttctgct gcgcgacggc tcggtggtgc aggtcgagaa tccgtccagg ctgccggatc
     4141 cggccaagat cgaggagatt cgcgagccga tcgtcagcgt cacgatcttc gtgccgcagg
     4201 aatacgtggg accggtgatg acgctgtgca ccggcaagcg cggggtgcag gtcgatcttg
     4261 cgtaccacag ccggcatgtg cacctgactt acgagctgcc gctggcggag atcgtgctgg
     4321 acttcttcga caagctcaag tcggtgtcgc gcggctacgc ctcgatggac tacgaattca
     4381 gggaatatcg cgcggccgac gtggtgaagg tcgacatgct gatcaacggc gaccgcgtcg
     4441 acgcgctctc cgtgatcgtg caccgaagca gttcgcagta ccgcggccgc gagatcgccg
     4501 cgaagatgcg cgcgctgatc ccccgccaga tgtacgacgt ggcgatccag gccgcgatcg
     4561 gcgcgaatat catcgcgcgc gagaacgtca aggcgctgcg caagaacgtg ctcgccaagt
     4621 gctatggcgg tgacatcacg cgcaagaaga agctgctcga aaagcagaag gccggcaaga
     4681 aacgcatgaa gcaggtcggc acggtggaga tcccgcagga ggcgttcctg gcgatcctgc
     4741 aagtcgagga aaaatgaatt tcgccctgat cctgttcatc cttctcgtcg tcagcttcgc
     4801 gatgtggctg ctggacctgc tgtggctgaa gaagacccgt tttgcggccg ccagcgccgc
     4861 gctggccgaa ttcgacgcgc gcaatgccgg caagatcggt gtcaacacgg cggcactcgc
     4921 cgccgaccgt tccgcgctgg aggaccggat cacgcgccgg ccgtggtacc tcgagtacac
     4981 ggcgagcttc ttcccggtga tcctgctggt gttcgtgctg cgctcgttct tcttcgagcc
     5041 cttccgcatc ccgtccgggt cgatgatccc gacgctgcag gtcggcgacc tcatcctcgt
     5101 gaacaagtac cagtacggca tccgactgcc ggtgatcaac aagaaggtca tcgacatcgg
     5161 ctcgccccag cgcggcgacg tgatggtgtt ccgctttccg cacaatccct cgcaggatta
     5221 catcaagcgc gtgatcgggc tgccgggcga ccgggtcgag tacctcgacc gtcgcctcac
     5281 catcaacggc aagccggtcg agaccgtcgg cgtcggccgc tacctagaag aagggcgact
     5341 gcagtactac gcggaagaca cggaaaaact gggccaggtc agccaccgga tcatcctgag
     5401 cgaaagcccg atgggcgtac cgatcatgtc gaccgggcag cacacgaatc cgga
//
LOCUS       DBNHBY010000393         9368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_226747, whole genome shotgun sequence.
ACCESSION   DBNHBY010000393 DBNHBY010000000
VERSION     DBNHBY010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_226747"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="AC16HB_09750"
     CDS             complement(<1..1298)
                     /locus_tag="AC16HB_09750"
                     /inference="COORDINATES: protein motif:HMM:NF015410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HIR5607112.1"
                     /translation="MASPTIVAGLKQILAPHAPFSRMADADLEFLIAHLQVAYYTPGE
                     TILAPSTEPSPHFFIVKQGQVRGLRDADPGKVAFEGGTGECFPVTALLAERPVALTYV
                     AVGDTFCLLLPRARFAQLTQRSSVFLDFCKRRLASLLDLSRQHLQANYAAEASAERTM
                     GMQLADLVRGPPVTCRPEETLRTAFERMHAAHVGSILVTEPAANGSEKLAGILTRTDL
                     IGRVILPGLALDTPVRAVMSPQVLSLPHDATAADATLVMAEHSIRHVPVVRATNGETR
                     VIGLVSERDLFTLQRLTVRQLAADLRRAEGVASLAATAADVRQLSYHLVAQGVAAGQL
                     TRLISHLNDQLTVRLLTLACAKFGVEASSFCWLALGSEGRGEQTIATDQDNGIIFSGE
                     ASEQAKWLALASWVNDALAECGFPLCKGNIMARNPQWCLTL"
     gene            complement(1311..2249)
                     /locus_tag="AC16HB_09755"
     CDS             complement(1311..2249)
                     /locus_tag="AC16HB_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5607113.1"
                     /translation="MSFLPGGATLVALIVFAVVITFIVNAVRIVPQQHAWVVERLGKF
                     HATLSPGLNIVIPFVDRIAYKHSLKEVPLDTPSQVCITRDNTQLHVDGVLFFQVTDPQ
                     RASYGSSNYIVAITQLAQTTLRSVIGKLELDKTFEEREFINHSVVSAIDEAARNWGVK
                     VLRYEIKDLTPPAEILRAMQAQITAEREKRALIASSEGRKQEQINIATGQREAAIAKS
                     EGEKQAQINNAQGQAAAVLAIAEATAEAIRKVAQSIQVPGGMEAVNLKVAERFVDAFA
                     GLAKTNNTLIVPANLADVGSLIATAMTVVRSQGRPA"
     gene            complement(2273..2701)
                     /locus_tag="AC16HB_09760"
     CDS             complement(2273..2701)
                     /locus_tag="AC16HB_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5607114.1"
                     /translation="MSAWVWWFVLGFVLLTAELMTGTFYLLVIALAFAAAGTTSLAGM
                     PFVIQLVVAAGIGLAGSLWLRNSRFGRRDAREAEPLQNLDVGQVVRIEQWSPSRTARA
                     AYRGAQWDVELAPGEEAIAGDFVIRDVLGSRLIVARRSAS"
     gene            complement(2712..5087)
                     /gene="ppsA"
                     /locus_tag="AC16HB_09765"
     CDS             complement(2712..5087)
                     /gene="ppsA"
                     /locus_tag="AC16HB_09765"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR5607115.1"
                     /translation="MQRGRYVIPFSGLRMTDVEQVGGKNASLGEMLSQLSAEGIRVPD
                     GFATTAEAFRRFLRANELEDRIHQRLARLDVSDVRALAEAGAQIRGWIVAAKFPADFE
                     EEIRLFYQWLTDGTSSEISLAVRSSATAEDLPDASFAGQQETFLNVHGIDAVVDRIRH
                     VFASLYNDRAIAYRVHKGFTHAEVALSAGVQRMVRSDLGASGVVFTIDTESGFKDVVF
                     ITASYGLGETVVQGAVNPDEFYVHKPMLALGKYPIIRRALGSKLLKMEFDPSKSDGRT
                     VRTVSVSDAERHRFALSDDDVLELARYAVTIENHYGRPMDIEWGKDGLDGRIYILQAR
                     PETVRSQLTAQVQHRYKLKETSTVLASGRAIGQKIGIGEVRIVRDIAEMDKVRDGDVL
                     VTDMTDPNWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGNATAALKDGALV
                     TVSCAEGDTGHVYDGRLSMEVETVQAGAMPSLPVKIMMNVGNPQLAFDFQALPNEGVG
                     LARLEFIINNNIGLHPKAVLDYPNVDAELKRAVESLAHGFANPRAFFESRLAEGIATI
                     AAAFWPKPVIVRLSDFKSNEYKKLIGGTRYEPDEENPMLGFRGAARFIAESFAECFRM
                     ECRALKRVRDDMGLANVEIMVPFVRTLTEARRVTELLAANGLKRGDNGLRLIMMCEVP
                     SNAILPEQFLEYFDGFSIGSNDLTQLTLGLDRDSGLVAEGFDERDPAVKSLLARAIDA
                     CRSRGKYIGICGQGPSDHPDFAQWLMERGIESISLNPDTVVETWRRLARTT"
     gene            5195..6034
                     /gene="ppsR"
                     /locus_tag="AC16HB_09770"
     CDS             5195..6034
                     /gene="ppsR"
                     /locus_tag="AC16HB_09770"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HIR5607116.1"
                     /translation="MPDRFLPAERTVFFVSDGTGITAETLGHSVMTQFEPVRYRKIRI
                     PFVDTADKAREAVARINETRFKDGKRPIVFSSLVDPFVNETLRQADALILDLFESFVE
                     PLETELGVKSTHTIGRSHTMSDSETYHRRIDAINFALAHDDGQSARSLEHADVILVGV
                     SRSGKTPTSLYLAMQYGIKAANYPLIPEDFERDQLPSALPPYRHKLFGLSIAPERLAE
                     VRHERRPNSRYASLENCRYEVAEAEKMMRREGIRCMSSTTKSIEEIAATILNEIGLER
                     RIG"
     gene            complement(6039..7298)
                     /locus_tag="AC16HB_09775"
     CDS             complement(6039..7298)
                     /locus_tag="AC16HB_09775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607117.1"
                     /translation="MWGESLRTRARKATARAVSDGLERLAESLRAGGGLPLEVILPDG
                     SRISFGLAPRVAMTIHDEATVAQFANPSLATLGEAFIEQRIDVDGDLMEVLPIADKLA
                     SAGGAPVTARVAAVLSRHTAKQDRADIGFHYDVGNDFYRLWLDERMIYSCAYFPRGDE
                     SINEAQVAKLDHICRKLRLAPGERLLDIGCGWGGLVMHAAQHYGVRAVGITLSDNQHA
                     LARERVAQANLSDRVEILLLDYREVVDRFGASSFDKVSSVGMFEHVGIRHLPQYFDIA
                     QRALRERGLFLNHGITAADVDNRPVGSGVGDFIAKYVFPHGELPHLHVAIREMSAHGF
                     EVFDVESLRPHYAKTLEHWSRRLEARLGEAAQSVDARRLRIWRAYLAGCAYGFAQGWM
                     NIYHVLASRQTQPGLTELPLSRAWIYR"
     gene            complement(7386..8177)
                     /locus_tag="AC16HB_09780"
     CDS             complement(7386..8177)
                     /locus_tag="AC16HB_09780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5607118.1"
                     /translation="MKPIASAANPNFRHWHKLATQPRSVRELGRTLAEGLHLAQAALA
                     ARTPVAAVLVRRGMSGGEAGRLVEAAVGTGAPAFELAPALFDRLSPVQTSVGLMLELL
                     TPPAALPAVIDTDLLYLDGVQDPGNAGALLRVAAAAGVTWVLAAPGTAALWAARTLRA
                     AQGAHFALHIVEGVGADVAARSFSGAWIAAAAHDAEPIWGAALPASAVGWVFGAEGQG
                     ISPAMLARCRVRVRVPLAAGVESLNVAAAAAVCLFERRRRLDGPQ"
     gene            complement(8243..8797)
                     /locus_tag="AC16HB_09785"
     CDS             complement(8243..8797)
                     /locus_tag="AC16HB_09785"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR5607119.1"
                     /translation="MKSRATAPSLFEAPARALGLVCGADEAGRGPLAGPVVAAAVILD
                     SKQPIEGLRDSKLLAPARRDVLATLIRARALAWAVAEASVPEIDALNILQASLLAMRR
                     AIEALQPAAQFALVDGNQVPHLAIPARAIVGGDAIEPAISAASILAKTHRDALMRDLD
                     RIHPGYGFAVHAGYPTPAHLARLR"
     gene            complement(8794..>9368)
                     /locus_tag="AC16HB_09790"
     CDS             complement(8794..>9368)
                     /locus_tag="AC16HB_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5607120.1"
                     /translation="VLPGSRRAEVDHIGPVFADAIEAMAAREPSLTFLIPAADARLRT
                     RIDALLATRMAARPRTRVVDGESHACLEAADVVLVASGTATLEAALYKKPMVIAYRMP
                     RVSAWIMRGIGGYLPYVGLPNILAGEFVVPELLQEEATPEALAHATLVQLNDEPGRLR
                     LAERFAQIHESLRRDTPALAAEAIIATMQR"
BASE COUNT         1547 a         3261 c         3032 g         1528 t
ORIGIN      
        1 agggtcaggc accactgcgg attgcgcgcc atgatgttgc ccttgcacag ggggaatccg
       61 cactcggcaa gtgcatcgtt gacccaactg gccagcgcaa gccacttcgc ctgttccgaa
      121 gcctcgccgc tgaagatgat tccgttgtcc tggtccgtcg cgatcgtctg ctcgccgcgc
      181 ccctcgctgc caagggccag ccagcagaac gaactcgctt cgaccccgaa cttcgcgcat
      241 gcgagcgtca gcagccgtac ggtcagctgg tcgttcagat ggctgatcag ccgcgtcagc
      301 tggcccgccg ccacgccctg cgcgaccagg tggtaggaaa gctgccgcac gtcggcggcg
      361 gtcgccgcga ggctcgcgac gccttcggcg cgccgcaggt cggcggccaa ttggcgcacg
      421 gtcagccgct gtagcgtgaa cagatcgcgc tccgacacca gtccgatcac gcgcgtctcg
      481 ccgttggtcg cgcgcacgac cggcacgtgc cggatcgaat gctccgccat caccagcgtc
      541 gcgtcggccg ccgtcgcgtc gtgcggcagc gaaagcacct gtggcgacat caccgcgcgc
      601 acgggcgtgt cgagcgccag cccgggcagg atcacgcgcc cgatcaggtc ggtacgcgtc
      661 aggatgccgg ccagcttctc gctgccattc gcggccggtt cggtgacgag gatcgaaccg
      721 acatgcgccg catgcatgcg ctcgaacgcg gtgcgcagag tttcttcggg ccggcacgtg
      781 accggcggcc cacgcacgag gtccgcgagc tgcatcccca tcgtgcgctc ggcgctggct
      841 tcggccgcgt agttggcctg caggtgctgg cgcgacaggt cgagcaggct cgccaggcgg
      901 cgcttgcaga aatcgaggaa cacgctgctt cgctgcgtca gctgcgcgaa gcgggcacgc
      961 ggcagcagca ggcagaacgt atcgccgacc gcgacgtagg tcagtgcaac gggccgctcg
     1021 gcaagcagcg ccgtgacggg aaagcactcg ccggtcccgc cctcgaatgc aaccttgccg
     1081 ggatcggcgt cgcgcagccc gcgaacctgc ccctgcttga cgatgaagaa gtgcggcgat
     1141 ggctcggttg acggtgcgag gatcgtctcg ccgggcgtgt agtaggcgac ctgcaggtgt
     1201 gcaatcagga actcgaggtc ggcatccgcc atgcgcgaga acggcgcatg tggtgcgagg
     1261 atctgcttca gccccgcgac gatcgtcggc gacgccatgg ccaccgtgcg ctaagcgggc
     1321 ctgccctgac tgcgtaccac ggtcatcgcc gtcgcgatca ggctgccgac atcggcgagg
     1381 ttcgcgggca cgatcagcgt gttgttggtc tttgccagtc cggcgaacgc atcgacgaaa
     1441 cgctcggcca ccttcaggtt caccgcttcc ataccgcccg gcacctggat cgactgcgcg
     1501 accttgcgga tcgcctcggc agtggcctcg gcgattgcca gcaccgcggc ggcctgcccc
     1561 tgcgcgttgt tgatttgcgc ctgcttttcg ccttcggact tcgcaatcgc cgcctccctc
     1621 tggccggtgg cgatgttgat ctgctcctgc ttgcggcctt cgctcgatgc gatcagcgcg
     1681 cgcttctcgc gctcggcggt gatctgcgcc tgcatcgcac gcaggatctc ggccggcggc
     1741 gtcaggtcct tgatctcgta gcgcagcact ttcacgcccc agttgcgtgc cgcttcgtcg
     1801 attgccgaca ccacgctgtg gttgatgaac tcgcgttcct cgaacgtctt gtccagttcc
     1861 agcttgccga tcaccgagcg cagcgtcgtc tgcgccaact gcgtgatcgc gacgatgtag
     1921 ttgctcgaac cgtaggacgc ccgctgcgga tcggtgactt ggaagaacag cacgccatcg
     1981 acgtgcagtt gcgtgttgtc gcgcgtgatg cagacctggc tcggcgtgtc gagcggaacc
     2041 tccttcaggc tgtgcttgta cgcaatgcgg tcgacgaacg gaatcacgat gttcagtccc
     2101 ggggacagcg ttgcatggaa cttaccgagc cgctcgacca cccacgcatg ctgctgcggc
     2161 acgatgcgga cggcattgac aatgaacgtg atcacgaccg cgaagacgat cagcgcgacg
     2221 agggtggcgc cacccggcag aaagctcatg aggttctccg tcttgttggg attcaggacg
     2281 ccgaacggcg cgccacgatc agccggctgc cgagcacgtc ccggatcacg aagtcaccgg
     2341 cgatcgcttc ctcgcccggc gccagttcca cgtcccactg cgcgccgcga taggctgcac
     2401 gcgcggtgcg gctcggcgac cactgctcga tccgcaccac ctgcccgacg tcgaggtttt
     2461 gcaggggttc ggcctcgcgc gcgtcacggc ggccgaaacg ggaattgcgc agccatagag
     2521 agccggccag cccgatgccg gcggcgacga ccagctggat cacgaacggc atgccggcga
     2581 ggctcgtcgt gcccgccgcg gcaaaggcca gagcgatcac gagcagatag aacgtgcccg
     2641 tcatgagctc cgcggtcagc agcacgaagc cgagtacgaa ccaccagacc cacgcgctca
     2701 cttgatctcc ttcaggtcgt acgcgccagc cggcgccagg tttcgactac ggtgtccgga
     2761 ttcagcgaga tcgactcgat cccccgctcc atcagccact gcgcgaaatc cggatggtcc
     2821 gacggcccct ggccgcagat gccgatgtac ttgccgcggc tgcgacaggc atcaatcgcg
     2881 cgcgcgagca ggctctttac ggccggatcg cgctcgtcga agccctcggc gacgagaccc
     2941 gaatcgcggt ccaggccgag agtgagctgc gtcagatcgt tggagccgat cgaaaagccg
     3001 tcgaagtact cgagaaactg ctccggcagg atcgcattgg acggtacctc gcacatcatg
     3061 atcagccgca agccattgtc accgcgcttc agcccgttgg cggccagcag ttccgtcacc
     3121 cggcgcgcct cggtcagcgt gcgcacgaac ggcaccatga tctcgacgtt ggcgagcccc
     3181 atgtcgtcgc gcacgcgctt caatgcccgg cattccatcc ggaaacactc ggcaaagctc
     3241 tccgcgatga aacgcgccgc gccgcggaag ccgagcatcg ggttctcctc gtccggctcg
     3301 tagcgcgtgc caccgatcag tttcttgtac tcgttgctct tgaagtccga caaccgcacg
     3361 atcaccggct tgggccagaa tgcggccgcg attgttgcga ttccctcggc cagccgtgat
     3421 tcgaagaacg cacgcgggtt ggcgaaccca tgcgccagcg attcgacagc gcgcttcagt
     3481 tccgcatcga cattcggata gtcgagcacg gctttcgggt gcaggccgat gttgttgttg
     3541 atgatgaact cgagccgtgc cagtccaacg ccctcgttcg gcagtgcctg gaagtcgaac
     3601 gccaactgcg ggttgccgac gttcatcatg atcttgaccg gaaggctcgg cattgccccg
     3661 gcctgcacgg tctcgacctc catcgacagc ctgccgtcgt acacgtggcc cgtgtcgccc
     3721 tcggcgcaag acaccgtgac gagcgcaccg tccttcagcg ccgccgtggc attgccgcag
     3781 ccgaccactg cgggaatgcc gagttcgcgc gcgatgatcg cggcgtggca cgttcgtccg
     3841 ccgcggttcg tgacgattgc cgcggcgcgc ttcatcaccg gctcccagtt ggggtcggtc
     3901 atgtcggtca ccagcacgtc gccgtcgcgc accttgtcca tctcggcgat atcgcgcacg
     3961 atccgcacct cgccgatgcc gatcttctgt ccgatcgcac ggcccgacgc cagcacggtc
     4021 gacgtctcct tcagcttgta gcggtgctgc acttgcgcag tgagctgcga cctcaccgtc
     4081 tcgggccgcg cctgcaggat gtagatgcgg ccatcgaggc cgtccttgcc ccactcgatg
     4141 tccatcgggc ggccgtagtg gttctcgatc gtcaccgcgt agcgtgcgag ctccagcacg
     4201 tcgtcgtcgc tcagcgcgaa acggtgccgc tcggcgtccg acacgctgac cgtgcgcacg
     4261 gtgcggccgt cggacttgga cgggtcgaac tccatcttca gcagtttcga accaagcgcg
     4321 cggcggatga tcgggtactt gcccagggcg agcatcggct tgtgcacgta gaactcatct
     4381 gggttgacgg cgccctgcac gaccgtttcg cccagcccgt agctcgccgt gatgaacacc
     4441 acgtccttga agccggattc ggtgtcgatc gtgaacacca cgcccgaggc gccgagatcg
     4501 ctgcgaacca tccgctgcac gcccgccgac agcgcgactt ccgcgtgcgt gaatcccttg
     4561 tgcacgcggt aggcgatcgc gcggtcgttg tacagcgacg cgaacacgtg cctgatgcgg
     4621 tcgaccacgg catcgattcc gtgcacgttc aggaaagttt cttgctggcc cgcgaacgac
     4681 gcgtccggca aatcctcggc cgtggcgctc gagcgcaccg cgagcgagat ttcacttgat
     4741 gttccatcag taagccactg ataaaaaagt ctaatttctt cctcgaaatc ggcaggaaac
     4801 ttggccgcga cgatccaacc gcggatctgc gccccagctt ccgcgagcgc acgcacatcg
     4861 ctcacgtcga ggcgcgccaa tcgctggtgg atacggtcct ccagttcgtt ggcacgcagg
     4921 aagcgccgga atgcctcggc cgtggtggcg aatccgtcgg gcacgcgtat gccctccgca
     4981 ctcaactgac tgagcatctc gcccaacgac gcattcttgc cgccgacctg ctccacgtcg
     5041 gtcatccgca gtccgctgaa tggaatcacg tacctgccgc gctgcattcc ctctcccatg
     5101 gttggcgcga ttggccacgc cgctgtgaca caatccgccg cgatcaaaga ccgcgttgcg
     5161 gtcgattcta acgaccggcc tcatcgcttc cttgatgccc gaccgattcc tgcccgccga
     5221 gcgcacggtg ttcttcgtct ccgacggcac cggcatcacg gccgagacgc tcggccacag
     5281 cgtgatgacg cagttcgagc cggtgcgcta ccgcaagatc cggattccct tcgtcgatac
     5341 ggccgacaag gcgcgcgagg ccgtggcgcg gatcaacgag acgcgtttca aggatggcaa
     5401 gcggccgatc gtcttctcca gcctggtcga cccgttcgtc aacgagacgc tgcgccaggc
     5461 cgacgcgctg atcctcgatc tctttgaatc gttcgtcgag ccgctggaga ccgaactcgg
     5521 cgtgaagtcg acacacacga tcggccgttc gcacacgatg tccgattcgg agacctacca
     5581 tcggcgcatc gatgcgatca acttcgcgct ggcacacgac gacggacagt ccgcacgcag
     5641 tctcgagcat gcggacgtga tcctggtcgg agtgtcgcgc agcggcaaga cgccgacgag
     5701 cctgtatctg gccatgcaat acggcatcaa ggcggccaac tacccgctga ttccggagga
     5761 tttcgagcgc gaccagttgc cgtcggcgct gccgccatac cgccacaagc tgttcgggct
     5821 gtcgattgca cccgagcggc tggccgaggt gcggcacgag aggcgcccca acagccgcta
     5881 cgcgtcgctg gagaactgtc gctacgaagt ggccgaggcg gaaaagatga tgcggcgcga
     5941 aggcatccgc tgcatgtcgt cgaccacgaa atcgatcgaa gaaatcgccg caacgatcct
     6001 gaacgagatc gggctcgaac gccggatcgg ctgaacggtc agcggtagat ccacgcccgc
     6061 gacagcggca actcggtcag gccaggctgt gtctgcctgc tggcgagcac gtggtagatg
     6121 ttcatccagc cctgcgcgaa tccataggcg cagcccgcca ggtaagcccg ccagatgcgc
     6181 aggcggcgcg cgtcgaccga ctgcgcggct tcgcccagcc gcgcctcgag ccggcgcgac
     6241 cagtgctcga gcgtcttcgc gtaatgcggc cgcaggcttt cgacatcgaa gacctcgaag
     6301 ccgtgcgccg acatctcgcg gatcgcgacg tgcagatgcg gcagttcgcc gtggggaaac
     6361 acgtacttcg cgatgaagtc gcccacgccc gaacccaccg gacggttgtc gacatcggct
     6421 gcggtgatgc cgtggttcag gaacagcccg cgctcgcgca gcgcgcgctg cgcgatgtcg
     6481 aagtactgcg gcaggtggcg gatgccgacg tgctcgaaca tcccgacgct cgacaccttg
     6541 tcgaaacttg aggcgccgaa tcgatcgacc acttcgcggt agtcgagcag caggatctcg
     6601 acgcggtccg aaagattcgc ctgcgcaacg cgctcgcgcg ccagcgcgtg ctggttgtcc
     6661 gacagcgtga tgccgaccgc gcgcacgccg taatgctggg cggcgtgcat gaccagcccg
     6721 ccccagccgc agccgatgtc gagcaggcgt tcgcccggcg cgagccgcag cttgcgacat
     6781 atatggtcga gcttggcaac ctgcgcctcg ttgatcgact cgtcgccgcg cgggaagtac
     6841 gcgcatgaat agatcatgcg ctcgtcgagc cacagccggt agaaatcgtt gccgacgtcg
     6901 taatggaagc cgatgtcggc gcggtcctgc ttcgcggtgt gccggctcag caccgctgcc
     6961 acgcgcgcgg tcaccggcgc gccgcccgcg ctcgcgagct tgtcggcgat cggcagcact
     7021 tccatcaggt cgccatcgac gtcgatgcgc tgctcgatga acgcctcgcc gagcgtggcg
     7081 agcgatgggt tggcgaactg ggcgacggtc gcttcgtcgt gaatcgtcat cgccacgcgc
     7141 ggcgccaacc cgaacgagat gcgcgatccg tccggcagga tcacctcgag cggcaggccg
     7201 ccgccggcgc gcaacgattc ggcgagccgt tccagcccgt cgctgacggc gcgtgccgtc
     7261 gccttgcgcg ccctggttcg cagagactca ccccacatcc tcgccctccg ctggtcttgc
     7321 cgcggtcccg attctctccg aagccaatac ggccgccaag tccgtgcact cacgcgtcga
     7381 cgccgctact gcggcccgtc caggcggcgg cggcgctcga acaggcagac cgccgccgcc
     7441 gccgcgacgt tcagcgattc gacgccggcc gccagcggaa cccggacgcg cacgcggcag
     7501 cgcgccagca tcgccgggga aatcccctgc ccctcggcgc cgaaaaccca gccgaccgcc
     7561 gatgccggca aagcggcgcc ccagatcggc tcggcgtcgt gcgcagcggc ggcgatccag
     7621 gcgccgctga atgaccgcgc cgccacgtcg gcgccgacgc cctcgacgat gtgcaacgcg
     7681 aaatgcgcgc cttgcgcggc acgcagcgtg cgcgccgccc atagcgcggc ggttcccggc
     7741 gccgccagca cccatgtcac gcccgcggcg gctgcgactc tcagcagcgc gcccgcattg
     7801 ccggggtcct gcacgccgtc caggtacagc agatcggtgt cgatcactgc gggaagcgct
     7861 gccggcgggg tcaggagttc aagcatcagg ccgacgctcg tctgcaccgg cgagaggcga
     7921 tcgaacagcg cgggcgcgag ttcgaatgca ggcgcgccgg tgcccacggc cgcttcgacc
     7981 aaccggccgg cctccccgcc ggacatgccg cgcctgacca gcacggccgc aacgggggtc
     8041 cgcgccgcca gcgcggcctg tgcgagatgc aggccttcgg ccagcgtgcg ccccaactcg
     8101 cgcacggagc ggggctgcgt ggcgagcttg tgccagtgcc ggaagttcgg attggcggcg
     8161 gacgcaattg gtttcatcgc gtcgattcaa gcaggatgcg caccggcgcg aacgagcgac
     8221 ggtgcgcggc acagggcccg cgctagcgca gccgcgccag atgcgcgggt gtcggatagc
     8281 cggcgtgcac ggcgaagccg tagccgggat ggatgcggtc caggtcgcgc atcagcgcgt
     8341 cccgatgggt cttggccagg atcgacgccg ccgagatggc gggctcgatc gcgtcgccgc
     8401 cgacgatcgc acgcgcagga atcgccagat gcggcacttg gttgccgtcg accagcgcga
     8461 actgcgccgc tggctgcagc gcttcgatcg cacgccgcat cgccagcagg ctcgcctgca
     8521 ggatgttcaa cgcgtcgatc tccggcacgc tcgcttcggc caccgcccag gccagcgcgc
     8581 gcgcgcggat cagcgtggcc agcacgtcgc gccgcgccgg cgcgagcagc ttggaatcgc
     8641 gcagcccctc gatcggctgc ttggaatcga ggatcaccgc cgcggcgacg accgggccgg
     8701 cgagcgggcc acggcccgct tcgtcggcgc cgcatacgag gccgagcgcg cgcgccgggg
     8761 cttcgaacag gctgggagcg gtggcgcgcg atttcatcgc tgcatcgtcg caatgattgc
     8821 ttcggcagcc agggcgggcg tgtcgcgccg caggctttcg tgaatctgcg cgaagcgctc
     8881 ggccagccgc aaccggcccg gctcgtcatt cagctgtacc agcgtcgcat gcgccagcgc
     8941 ctccggcgtg gcctcctcct gcagcagttc gggaacgacg aattcgcccg caagtatgtt
     9001 cggcagccca acataaggca ggtagccgcc gatgccccgc atgatccacg ccgacacgcg
     9061 cggcatgcgg taggcgatca ccatcggctt tttgtacaag gccgcttcca gcgtcgcggt
     9121 gccgctggcg accagcacaa catcggctgc ttccaggcag gcatgcgact cgccgtcgac
     9181 cacgcgcgtg cgcgggcgcg cggccatgcg tgtcgccagc aacgcgtcga tgcgcgtgcg
     9241 cagccgcgca tccgcggcgg gaatgagaaa tgtcaggctc ggctcgcgcg cagccatcgc
     9301 ctcgatcgca tcggcgaaca ccggaccgat gtgatcgacc tcggcgcgcc ggctgccggg
     9361 cagtacgg
//
LOCUS       DBNHBY010000394         7350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1173280, whole genome shotgun sequence.
ACCESSION   DBNHBY010000394 DBNHBY010000000
VERSION     DBNHBY010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1173280"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16HB_09795"
     CDS             complement(<1..347)
                     /locus_tag="AC16HB_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5607121.1"
                     /translation="MKKLILASLLAGAGAVSSVALAQGYVGVGAGQSDFKVDCAGLSN
                     CDTKDTGYKVFGGYMFTPHFGVEGAYFDLGKAKGSGTGDPLLGTFNVSGKADGFGLYG
                     VALAPLDSFNVFA"
     gene            complement(433..1368)
                     /locus_tag="AC16HB_09800"
     CDS             complement(433..1368)
                     /locus_tag="AC16HB_09800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607122.1"
                     /translation="MNTGFLYAVCVLIWGTTWLGIKFQLDALAPEPGVALRFLCAAAL
                     LALYCRRRGITLRFDARLHALFALQGLAGFCVSYVFIYHAERFIVSGLVAIGYAASPL
                     ANMLLARLLFGTALSRRIFFGGVLGLAGIALIFLHELMRVSLNDTLLTGVALTMTAVL
                     LSSVAGMAATRYHQFHVHGWAPLAWAMLYGGAATALFALAAGRSFAIACSWTFAASFA
                     YLVVGGSILAFGAFYALVHRIGVARAGYVGVMTPIVALVVSSLFEGFAWTAVTLVGVA
                     LAVAGNGIALGPRALGLASKSRVGALQSSNDRRSN"
     gene            complement(1401..1793)
                     /locus_tag="AC16HB_09805"
     CDS             complement(1401..1793)
                     /locus_tag="AC16HB_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5607123.1"
                     /translation="METAIPREHLEEFRQQLLRLRAAITAAVDARLHGQDEDRRDEAG
                     LPQRAAETDDDGAAETARLADLAHLSRNADELSRIDAALTRIADGSYGICIDCGETIG
                     TQRLRAYPAALRCAACQGTFERRPGVRV"
     gene            1995..4754
                     /locus_tag="AC16HB_09810"
     CDS             1995..4754
                     /locus_tag="AC16HB_09810"
                     /EC_number="2.3.1.-"
                     /EC_number="6.2.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HIR5607124.1"
                     /translation="MFTSHYLRAALVPRSVAVVGATDREDSLGKYVFDNIVGGEFKGN
                     VYPVNPKHDRIGTHTCYQSLAALPEAPDLAVIVTPARTVPDLIDDAGTRHIPAVLVLS
                     AGFAEIGPGGKRLQELALSRARAHGIRMLGPNCLGLMRPEIGLNATFARTAARPGSVA
                     LVSQSGAVVAALLDYAWTAGFGFSSVVSTGGGSDVEFSEILDFLALDSSTKSIVLYVE
                     GVHDARAFLSSVRAAASVKPVVVLKVGRHMTGSKAAMSHTGALVGDDAVFHMALRRVG
                     AIRVTAYTQMYAAAETLAAGRLPRPAPGNRLAIVTNGGGPGVLAADATADNTVALAKL
                     APQTIEQLDKLLPSTWSRGNPVDIIGDADANRFAQALQIVLNDDGNDGVLVLFCPTIR
                     LKAEDTARALLPVAQASEKPVVSAWLGAEDAAKGRAVFEGAGMPALISPERGVESFGY
                     LAKYVRNRELRLQVPPPRVDEFNLNVDEARRIVGRALVSGRALLNEKESKDLIAAFGI
                     ETATTQVAKSAEEAVALAEAVGYPVALKVFALGITHKSEVGGVLLSLRTPEDVAHAFE
                     TIRARVAERAPNAEFVGVLVQKMIVRPHGRELIVGLARDPVFGPVLSFGMGGIAVEVF
                     RDSAVALPPLNRFLAQELISRTRVAKMLDNFRGLPGVDLDAVVNVLLKVSELACELPC
                     IHELDINPLLADENGAVALDARIVLGDGALVPDPTYSHLAIHPYPKNLVRATRLKTGQ
                     TVLLRPIRPEDAQAEKRLISRLSQRSMYMRFHAPLRELTAERLVRFTTIDYDREMAFV
                     AIDSSGAEEEIRGVARYTRNPDGTTCEFGVVVEDEWQGRGLGHALMHALESCARERGL
                     TEIIGYVLKENEEMARLMLGRTYHAERDEDDAGVVRFIKELHLQAGSGVPLSKEDART
                     LTGAS"
     gene            4751..5671
                     /locus_tag="AC16HB_09815"
     CDS             4751..5671
                     /locus_tag="AC16HB_09815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR5607125.1"
                     /translation="MIARSSLRLLAAAVGALWLSCSLAQGPVAADAAPKRGFMWEARK
                     GARSVLLLGTIHVGKPQFIAPDPGTLALLSRADAIAVEADVSEAARVAPIVQRLAYYG
                     KDEPGLDVRAPALKPRLEEFARRDGLSAELFWRMKPWMVANTLVVAEATRHGYSPAYA
                     TEAFLFDFARRTGKPIVEIESIEAQLRLFDASSESTQIAYLRQALDSIDNGTNQDEVA
                     RLVDAWDKRDGAAMERLLARMRAEKGEAERFVVEKIIDGRHPRMVEAIERYAASGKRY
                     AVAVGSLHYFGPNGLLELLKQRGYSIDPVP"
     gene            5671..6330
                     /gene="pdeM"
                     /locus_tag="AC16HB_09820"
     CDS             5671..6330
                     /gene="pdeM"
                     /locus_tag="AC16HB_09820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HIR5607126.1"
                     /translation="MRLPIGGRMRLLAQRAVLIETTASLVLADAHFGKAAAFRARGVP
                     VPRGTTEDNLARLDGLIGEFNPGKLILLGDLFHARESHAPRTVAAMRAWRQKHAALEI
                     VLVEGNHDRHAGTPPAALGIRVEQEPWHSDGLAFCHHPQQIANTTVVAGHLHPCVRLH
                     GRADQSVRLPCFWLRHGLVVLPAFGEFTGGAAIAREDGDRVIAIAEDRLFEIPALSRA
                     A"
     gene            complement(6314..7126)
                     /locus_tag="AC16HB_09825"
     CDS             complement(6314..7126)
                     /locus_tag="AC16HB_09825"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5607127.1"
                     /translation="MTTLPTLFLSHGSPMHALNAGRAGVGWAQLAERIGKPRALLMAS
                     AHWESEWPMLTTSSRPDTIHDFGGFPDELYKLRYPAPGAPDVAQRAIDLLKAAGMSAS
                     ANGCRGLDHGAWVPLRHMYPAADVPVVQLSLQTQLPTAHTLRVGRLLAPLAAEGVLMI
                     GSGHMTHNLREWIEFARRHRMQVSEAATAPYVEEFRAWVDSALRGDDAERIARWSDEA
                     PHAQRAHPSDEHFLPLPFAFGAAGPRPQVERIDLGVDSGVLAMDAYVFTPRG"
BASE COUNT         1189 a         2496 c         2529 g         1136 t
ORIGIN      
        1 ttggcgaata cgttgaagct gtcgagcggt gccagcgcga ctccgtaaag accgaagcca
       61 tccgctttgc cgctgacgtt gaacgtgccc agcagcgggt cgcccgtgcc ggaccctttc
      121 gccttgccga gatcgaaata cgcaccttcg acgccgaagt gcggcgtgaa catgtaaccg
      181 ccgaacacct tgtagccggt gtccttggtg tcgcaattgc tcaacccggc gcagtcaacc
      241 ttgaaatccg actgcccggc accaacgccg acatagcctt gcgcgagcgc gactgacgaa
      301 acggcgcctg ctccagcgag gagcgacgcg aggatcagtt tcttcataga acctccgtgc
      361 caattgccag ccatatcgat ccaaccagca gtcgcactct gagccggatg gcgaaacgct
      421 tgccggcaag ggtcagtttg aacggcgatc gttgctcgat tgcaacgcgc cgacgcggga
      481 ctttgacgcg aggccaagtg cccgcgggcc cagcgcaatg ccattgcctg ccaccgcgag
      541 cgccacgccg acgagcgtca ccgcggtcca cgcgaaaccc tcgaacaatg acgacacgac
      601 caacgcgacg atcggtgtca tcacaccgac gtagccggcg cgtgccaccc cgatccggtg
      661 caccagcgcg tagaacgcgc cgaacgcgag gatcgagccg ccgacgacga ggtaggcaaa
      721 cgatgctgca aacgtccagc tgcatgcgat tgcgaacgac ctgcccgcgg caagggcaaa
      781 gagtgccgtc gccgcgccgc cgtacagcat cgcccacgcc agcggcgccc agccgtgcac
      841 gtggaactgg tgatagcgcg tcgccgccat gccggccacg ctcgacagca gcaccgcggt
      901 catcgtcagc gccacgcctg tgagcagcgt gtcgttgagt gatacgcgca tgagctcgtg
      961 caggaagatc agcgcgatgc cggccaggcc cagcacgccg ccgaagaaga tgcgccgcga
     1021 cagcgcggtg ccgaacagga gccgcgcgag cagcatgttc gccagcggcg aggccgcata
     1081 gccgatcgcg accaggcccg aaacgatgaa ccgctcggcg tggtagatga agacgtaact
     1141 gacgcagaag cccgccagac cctgcagcgc gaacagtgcg tgcaaccgcg cgtcgaaacg
     1201 cagcgtgatg ccgcggcggc gacagtagag cgcaagcagg gccgccgcac acaggaagcg
     1261 cagcgccact cccggttcgg gcgccagtgc gtcgagttgg aacttgatgc cgagccaggt
     1321 cgtgccccag atcagcacgc ataccgcgta caggaagccg gtgttcacgc ctcctcagta
     1381 ccccagggaa tgcggaaacg ctacacgcga acgccgggcc ggcgctcgaa ggttccctgg
     1441 caggccgcac agcgcagcgc ggccggatag gcgcgcagcc gctgcgtgcc gatggtctcg
     1501 ccgcaatcga tgcagatgcc gtagctgccg tcggcgatgc gcgtcagcgc cgcgtcgatc
     1561 cggctcagtt cgtcggcgtt gcgcgacagg tgcgcgaggt ccgccagccg cgccgtttcc
     1621 gctgctccgt cgtcgtccgt ttcggcggcc cgttgcggca ggcccgcctc gtcgcgccga
     1681 tcctcgtcct ggccgtgcag gcgcgcgtcg actgccgccg tgatcgccgc gcgcagacgc
     1741 agcagttgct ggcggaattc ctccaaatgt tcgcgcggga tcgccgtttc catcgttttc
     1801 ctcgcggtgc ctgcaccttc atgcagcgtt ctgcaccagc gcggctttca ggctacgcag
     1861 ccacgcctta tcccgattga ccggcatcaa accggagggg ccgctctagc cacccgcttg
     1921 gcctccccgc tgccggcctg taacgtttgc ggtgacgttc gaagtgcgcg catccatcgc
     1981 gccaaagcaa cccgatgttc acctctcact atctgcgtgc cgcgctggtg ccgcgctcgg
     2041 tggccgtggt cggtgccacc gaccgcgaag attcgcttgg caagtacgta ttcgacaaca
     2101 tcgtcggcgg cgaattcaag ggcaatgtct atccggtcaa tccgaagcac gaccgtatcg
     2161 gcacgcacac ctgctatcag tcgctggccg cgctgccgga agcgcccgac ctggcagtca
     2221 tcgttacgcc ggcgcggacg gtgccggatc tgatcgacga cgccggcacg cgccacatcc
     2281 cggccgtact cgtgctgtcg gccggctttg ccgagatcgg ccccgggggc aaacgcctgc
     2341 aggaactcgc gctgtcccgc gcgcgcgcgc atggcattcg gatgctggga ccgaactgcc
     2401 tcggtctgat gcgccccgag atcgggctga acgcgacctt tgcacgcacg gcggcgcgcc
     2461 ccggttcggt tgcgctggtg tcgcaatcgg gggcggtggt cgccgcgctg ctcgactacg
     2521 catggaccgc gggcttcggc ttctcgtccg tggtgtccac cggcggtggt tccgacgtcg
     2581 aattttccga gatcctcgat tttctagcgc tcgactcgtc aacaaagagc atcgtgctgt
     2641 acgtggaagg cgtgcacgat gcgcgcgcgt tcctgtcgag cgtgcgtgcc gctgcgagcg
     2701 tcaaaccggt cgtcgtgctg aaggtcggcc gccacatgac cggttccaag gcggcgatga
     2761 gccacaccgg tgcgctggtc ggcgacgacg cggtgttcca catggctttg cggcgcgtgg
     2821 gcgcgattcg cgtcaccgcc tacacgcaga tgtatgcagc ggcggaaacg ctcgccgcgg
     2881 gtcggctgcc gcgcccggcg ccgggcaacc ggctggcgat cgtcaccaac gggggcggcc
     2941 ccggtgtgct ggccgccgac gcaaccgccg acaacacggt cgcgctggcc aaactcgcac
     3001 cgcagacgat cgagcagctc gacaagctgt tgccgtcgac gtggtcgcgc ggcaacccgg
     3061 tcgacatcat cggcgacgcc gacgcgaacc ggttcgcgca ggcgctgcag atcgttctga
     3121 acgatgacgg caacgacggc gtgcttgtgc tgttctgccc cacgatccgg ctgaaggcgg
     3181 aggacaccgc gcgcgcgttg ctgccggtgg cgcaggcctc cgagaagccg gtggtcagcg
     3241 cgtggttggg cgccgaggac gcagccaagg gccgtgctgt gttcgagggt gccggcatgc
     3301 cggcgctgat cagcccggag cgcggcgtcg aatcgttcgg atatcttgcc aagtacgtgc
     3361 gcaaccgcga actgcggttg caggtgccgc cgccgcgcgt cgacgagttc aacctgaatg
     3421 tcgacgaggc gcgccgcatc gtcggccgcg cattggtgtc cggacgcgcg ctgctgaacg
     3481 agaaggaatc gaaggacctg atcgccgcat tcggcatcga gactgcgacc acgcaggtgg
     3541 cgaagtcggc cgaggaggcg gtcgcgctgg cggaagcggt cggctacccg gtcgcgttga
     3601 aggttttcgc gctcggtatc acgcacaaga gcgaagtcgg cggggtgctg ctgtcgctgc
     3661 gcacgccgga ggatgtcgcg catgccttcg agaccatccg cgcgcgggtg gcggagcggg
     3721 cgccgaacgc cgagttcgtc ggcgtcctgg tgcagaagat gatcgtgcgc ccgcatgggc
     3781 gcgagctgat tgtcggcctg gcacgcgatc cggtgttcgg gccggtgctc tcgttcggca
     3841 tgggcggcat cgcggtcgag gtgttccgcg actccgcggt ggcgctgccg ccgctgaacc
     3901 gttttctggc gcaggaactg atctcgcgca cgcgcgtggc gaagatgctc gacaatttcc
     3961 gcgggctgcc gggtgtcgac ctcgatgcgg tggtcaacgt actgctcaag gtgtccgaac
     4021 tcgcgtgcga gctgccgtgc atccacgagc tcgacatcaa tccgctgctg gcggacgaga
     4081 acggggcggt ggcgctcgac gcgcgcatcg tgctcggcga cggcgcgctg gtgccggacc
     4141 cgacgtactc ccacctggcg atccatccgt atccgaagaa cctggtgcgc gcgacgcggc
     4201 tgaagaccgg ccagaccgtg ctgctgcgcc cgatccgccc cgaggatgca caggccgaga
     4261 agcgcctgat atcgcggctg tcgcagcgct cgatgtacat gcgcttccat gcaccgctgc
     4321 gcgagctgac ggccgagcgg ctggtgcgct tcaccacgat cgactacgac cgcgagatgg
     4381 cattcgttgc gatcgactcc agcggcgcag aggaggagat ccgcggcgtg gcgcgctaca
     4441 cgcgcaatcc ggacggcacg acgtgcgagt tcggcgtggt ggtcgaggac gaatggcagg
     4501 gccgcggcct cggccacgcg ctgatgcatg cgctcgagag ctgcgcgcgc gagcgcggcc
     4561 tgaccgagat catcggctac gtgctgaagg aaaacgagga aatggcgcgg ttgatgctcg
     4621 gccgcaccta tcacgccgag cgcgacgagg acgacgccgg cgtggtgcgc ttcatcaagg
     4681 aactgcacct gcaggccggc tctggcgtgc cgctgtcgaa ggaagacgcg cgcacgctca
     4741 ctggcgcgtc gtgatcgcgc gcagttcact gcgcctgctc gccgccgcgg tcggcgcgct
     4801 gtggctgtcg tgctcgcttg cccagggtcc ggtcgccgcc gacgcggcgc cgaagcgcgg
     4861 cttcatgtgg gaagcgcgca agggcgcgcg gagcgtgctg ctgctcggca cgatccacgt
     4921 cggcaagccg cagttcattg ccccggaccc cggaaccctc gcgctgcttt cgcgcgccga
     4981 tgccatcgcc gtcgaggccg acgtgtcgga agcggcgcgc gtggcgccga tcgtgcagcg
     5041 gcttgcctat tacggcaagg acgagccggg gctcgatgtg cgcgcgccgg cgctgaaacc
     5101 gcggctggag gaattcgcgc gccgcgacgg cctgtcggcc gaactgttct ggcgcatgaa
     5161 gccgtggatg gttgccaaca cgctggtcgt ggccgaggcc acgaggcacg gctatagccc
     5221 ggcctacgcg accgaggcgt tcctgttcga cttcgcgcgc aggaccggca agccgatcgt
     5281 cgagatcgaa agcatcgaag cgcagctgcg cctgttcgat gcaagctccg agtcgacgca
     5341 gattgcgtac ctgcggcagg cgctcgactc gatcgacaac ggaacgaacc aggacgaggt
     5401 cgcacggctc gtcgacgcgt gggacaagcg cgacggcgcg gcgatggagc ggctgctcgc
     5461 caggatgcgt gccgagaagg gagaggccga acgcttcgtc gtcgagaaga tcatcgacgg
     5521 tcgacacccg cgcatggtcg aggcgatcga gcgctatgcc gccagcggca aacggtacgc
     5581 ggtcgcggtc ggatcgctgc actacttcgg gccgaacggg ttgctcgaac tgttgaagca
     5641 gcgcggctac tcgatcgatc cggtgccgtg atgcggctgc cgatcggcgg ccgcatgcgc
     5701 ctgctggcac aacgagccgt cctcatcgaa accaccgcga gcctcgtcct tgccgacgcg
     5761 cacttcggca aggcggcggc gtttcgcgcg cgcggcgtgc cggtgccgcg cggcacgacc
     5821 gaggacaacc tggcgcgact cgacgggctg atcggggaat tcaacccggg caagctgatc
     5881 ctgctcggcg acctcttcca tgcgcgcgaa tcgcacgcgc cgcgcacggt cgcggccatg
     5941 cgcgcatggc gacagaagca cgccgcgctc gagatcgtgc tcgtcgaggg taaccacgac
     6001 cgccacgccg gcacgccgcc cgccgcgctc ggcatccgcg ttgagcagga gccgtggcac
     6061 agcgacggcc tggctttctg tcatcacccg cagcagatcg cgaacacgac cgtggtcgcc
     6121 ggtcatctgc atccgtgcgt acggttgcac ggccgcgccg accagagcgt gcggctgccg
     6181 tgcttctggc tgcgccatgg gctcgtcgtg ctgccggcat tcggcgagtt caccggcggc
     6241 gcggcgatcg cgcgcgagga cggcgaccgc gtgatcgcga tcgccgaaga ccggctgttc
     6301 gagattcccg cgctcagccg cgcggcgtga agacgtaggc gtccatcgcc agcacgccgc
     6361 tgtcgacgcc gaggtcgatg cgctcgacct gcggccgcgg gccggcggcg ccgaacgcaa
     6421 acggcagcgg caggaagtgc tcgtcgctgg gatgcgcgcg ttgcgcgtgc ggtgcctcgt
     6481 cgctccagcg cgcgatgcgc tcggcatcgt cgccgcgcag ggcgctgtcg acccaggcgc
     6541 ggaactcctc gacgtacggg gcggtcgcgg cttcgctgac ctgcatgcgg tggcggcgcg
     6601 cgaattcgat ccactcgcgc aggttgtgcg tcatgtggcc cgagccgatc atcagcacgc
     6661 cctcggccgc aagcggcgcg agcagccggc cgacccgcag cgtgtgcgcc gtcggcaatt
     6721 gcgtctgcag cgacagctgc acgaccggca cgtcagcggc cggatacatg tgccgcagcg
     6781 gaacccacgc gccgtgatcg agcccgcggc acccgttggc gctggccgac atgccggcgg
     6841 ccttgagcag atcgatcgcg cgctgcgcca cgtcgggcgc gcccggcgcc ggataacgca
     6901 gcttgtacag ctcgtccgga aaaccgccga agtcgtgaat cgtgtccggc cggctgctcg
     6961 tcgtcagcat cggccactcg ctctcccagt gcgccgatgc catcagcagc gcgcgtggct
     7021 tgccgatccg ctccgcgagc tgcgcccagc cgacgccggc gcgaccggcg ttcagcgcgt
     7081 gcattggcga accgtgggaa aggaagagtg tggggagcgt ggtcatgatc gtctttcagt
     7141 gcgagccagc gggcggcagt ccgaggccgg cgccacccgc cgatttcaag cggttattac
     7201 tgcaacaggc cctcggcgcg caacgttccc tgcactttcg ggcgcgcggc gacccgcctc
     7261 acgtcatcgg acagctccgg ccattgcgcc aggtcgatgc cagtccactg acggtagtcg
     7321 ccgccgcttt ccatctgcat ggttccggtg
//
LOCUS       DBNHBY010000395         3267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1782033, whole genome shotgun sequence.
ACCESSION   DBNHBY010000395 DBNHBY010000000
VERSION     DBNHBY010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1782033"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16HB_09830"
     CDS             <1..420
                     /locus_tag="AC16HB_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR5607128.1"
                     /translation="FSIGAGALAVMRTRTDNRGVSDSTRLEPVRTRRGSTLARALAIA
                     YLLLVVYVSLAPWSGWRDIGVSAFAYLSAPLPRYITAFDIVVNVLGYLPLGALAVLAL
                     HPHVRGAAAVVLATAGGVLLSGAMEALQTFLPSRVAS"
     gene            550..1200
                     /locus_tag="AC16HB_09835"
     CDS             550..1200
                     /locus_tag="AC16HB_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607129.1"
                     /translation="MPLALVALWPLAQIHPTAMLFGNGEMRDLLHRIVGAFGEWPGWF
                     AASRFGPAEFILDEAAVTAAGLLAAGLTLAAIMQSLAPRARVLLALLALALATKSIAL
                     GMQFGAARVFAWMTPGAVGGLSLGTLALVVAATGRPRAMARLALLATLVLVTLVNLTP
                     ENPYHAHWVQQWHPGRLVHAAQGADWLATAWPYALLAWLARAAFTARLPKWNAPVP"
     gene            1231..1536
                     /locus_tag="AC16HB_09840"
     CDS             1231..1536
                     /locus_tag="AC16HB_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR5607130.1"
                     /translation="MYYKHHVFFCLNQRDDGRPCCASHDAAAMQAHAKERVKAAGASG
                     KGGIRINKSGCLDRCEEGPCVVVYPEGVWYTYVDTHDIDEIVDSHLLNGKVVERLKL"
     gene            1553..2224
                     /locus_tag="AC16HB_09845"
     CDS             1553..2224
                     /locus_tag="AC16HB_09845"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5607131.1"
                     /translation="MLKVERRLIDGPAGRIEVALDVPAHSPKGLAFIGHPHPLYGGTL
                     ENKVAATLARAFAALGWLAVRPNFRGVGRSEGTHDHGQGETDDFVHVIEAAPRWVSDF
                     ALADGLPLALAGFSFGSFVAARAAARLAERGRAPNHLVLVGAAAGKWDMPAVPPNTLL
                     IHGEHDETIALADVFEWARPQSLPVIVLPGTDHFFHRRLSLLKRLVQLHIVAERSIDA
                     VGQND"
     gene            2279..2848
                     /locus_tag="AC16HB_09850"
     CDS             2279..2848
                     /locus_tag="AC16HB_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607132.1"
                     /translation="MNEARVFRKTQAGQAEVTARSGSLSLAMRRVLILIDGRRPMSEM
                     APMVRPGEIDGMIAALLEKGYIEPVIAAPAPVPAPAPGAAGPTTVSGPSTVSGPRTVA
                     PTITQGPRTLSDVSDPGRFMSIDEAKRRVVRELTDRLGPEAEYIAIKIEQCGTLEELR
                     ARIRDAERVLTTAMGEKVAQDFVRALRRR"
     gene            2883..>3267
                     /locus_tag="AC16HB_09855"
     CDS             2883..>3267
                     /locus_tag="AC16HB_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5607133.1"
                     /translation="MEWHQATCYLNGEFVPLANAKIPVLDRGFIFGDGVYEVIPCDQA
                     QPGFTAPFRAREHFDRLDRSLGQVRISNPLSRDHWLQLVARLIDLHPWPRQSIYLQVT
                     RGVAKRDHVFPAGVTPTVFAVSSPWP"
BASE COUNT          456 a         1146 c         1144 g          521 t
ORIGIN      
        1 tttagcatcg gggccggcgc gcttgctgtg atgcgcacgc gcaccgacaa tcgcggcgtg
       61 tccgacagca ctcgcctcga gcccgtgcgg acgcgccgcg gctcaacgct ggcgcgcgcg
      121 ctcgccatcg cctatctgct gctggtcgtc tacgtcagcc tcgcgccttg gtccggctgg
      181 cgcgacatcg gcgtgagcgc gttcgcgtat ctgtccgcgc cgctgccgcg ctacatcacc
      241 gcgttcgaca tcgtcgtcaa cgtgctcggc tacctgccgc tcggcgcgct cgcggtcctg
      301 gcgcttcacc cgcacgtgcg cggcgccgct gcggttgtgc tcgccaccgc cggaggcgtg
      361 ctgctgtcgg gcgcgatgga agcgctgcag acgttcctgc caagccgcgt cgcgtcctag
      421 ctcgatctgg cggcgaacag cggcggcgcg ctgctcggtg cgctggccgc ggcgccctac
      481 gcagcgagcc tgatcgaccg cggccgcctt gccgagctgc gcgcgcgctg gttccgccgc
      541 gacgcgtccg tgccgctggc gctggtcgcg ctgtggccgc tggcgcagat tcatccgacg
      601 gcgatgctgt tcggcaatgg cgagatgcgc gacctgctgc accggatcgt cggcgcgttc
      661 ggcgaatggc cgggctggtt cgctgccagc cgcttcggcc cggccgaatt cattctcgac
      721 gaagccgcgg tcacggcggc cggcctgctc gccgcagggc tgactttggc ggcgatcatg
      781 cagtcgctcg cgccgcgcgc gcgcgtgttg ttggcgttgc ttgcgctggc gctcgcgacg
      841 aagtcgatcg cgctcggcat gcagttcggc gccgcgcgcg tgttcgcatg gatgaccccg
      901 ggcgcggtcg gagggctgtc gctcggcacg ctcgcgctcg tcgttgcggc gacaggacgg
      961 ccgcgtgcca tggcgcggct ggcgctgctg gccacgctcg tgctggtcac attggtcaac
     1021 ctgacaccgg agaatccgta ccacgcgcac tgggtgcagc agtggcatcc gggacggctc
     1081 gtgcacgcgg cgcagggcgc cgattggctg gcgaccgcgt ggccgtacgc actgctcgcc
     1141 tggctcgcgc gcgccgcgtt cacggcgcgg ctgccgaaat ggaacgcgcc agttccgtaa
     1201 actggcgtca tcgcgagtcc ggatcggatc atgtactaca agcatcacgt gttcttctgc
     1261 ctgaaccagc gcgacgacgg ccggccgtgc tgcgccagcc acgacgccgc ggcgatgcag
     1321 gcgcacgcaa aggagcgcgt gaaggcggcc ggcgccagcg gcaagggcgg catccgcatc
     1381 aacaagtcag gctgcctcga ccgctgcgag gaaggaccgt gcgtggtcgt gtacccagag
     1441 ggcgtctggt acacgtacgt cgacacgcac gacatcgacg aaatcgtcga ttcgcacctg
     1501 ctcaacggca aggtcgtcga gcggctgaag ctctgagcgc gctgacacga ccgtgctgaa
     1561 agtcgaacgc cgactgatcg acggcccggc gggccgcatc gaggtcgcgc tcgacgtgcc
     1621 cgcgcactcc cccaaagggc tcgcgttcat cggccatccg catccgttgt acggcgggac
     1681 gctcgagaac aaggtcgcgg cgacgctggc acgcgcgttt gccgcgctgg gctggctcgc
     1741 ggtgcggccc aacttccgcg gcgtcggccg cagcgaaggc acgcacgacc acggccaggg
     1801 cgagaccgac gacttcgtgc acgtcatcga agcggcgcca cgctgggtgt cggacttcgc
     1861 actcgcggac ggtctgccgc tcgcgctggc cgggttctcg ttcggcagct tcgtcgccgc
     1921 gcgcgcagcc gcgcggctcg ccgaacgcgg tcgcgcgccg aaccacctgg tgctggtcgg
     1981 agccgccgcc ggcaagtggg acatgccggc ggtgccgccc aatacgctgc tgatccacgg
     2041 cgagcacgac gagacgatcg cgctcgccga cgtgttcgag tgggcacggc cgcagagcct
     2101 gcccgtcatc gtgctgccgg gcaccgatca cttcttccat cgccgcctgt cgctgctgaa
     2161 gcggctcgtg cagttgcaca tcgtggctga aaggtcgatt gacgctgtcg ggcaaaacga
     2221 ctagaagttg ccggacgcgt ggactcgcgt ccggcgtcat gcaagcggtc gaggatcgat
     2281 gaacgaagcg cgcgtgttcc gcaagaccca ggccggccag gccgaggtca ccgcgcgcag
     2341 cggttcgctg tcgctcgcga tgcggcgcgt gctgatcctg atcgacggca ggcggccgat
     2401 gtccgagatg gcgccgatgg tgcggcccgg cgagatcgac ggcatgatcg ccgcgctgct
     2461 cgagaagggc tacatcgaac cggtcatcgc cgccccggca ccggtccccg cgcccgcacc
     2521 cggcgctgcc gggccgacca cggtttccgg accgtcgacc gtctccggac cgagaactgt
     2581 cgcaccgacg attactcagg gtccgcgcac gctgtcggac gtctcggatc ccggccgctt
     2641 catgtcgatc gacgaagcca agcggcgcgt cgtgcgcgaa ctcaccgacc gcctcggccc
     2701 cgaggccgag tacatcgcga tcaagatcga gcagtgcggc acgctcgaag aactgcgcgc
     2761 gcgcatccgc gacgccgagc gcgtgctcac cactgcgatg ggcgagaagg tcgcacagga
     2821 tttcgtgcgc gcgttgcggc ggcggtagca tccgcgcttt cgaccggcgc gggagttcgt
     2881 tgatggaatg gcaccaggcg acctgctacc tgaacggcga gttcgtgccg ctggcgaacg
     2941 cgaagattcc ggtgctcgac cgcggcttca tcttcggcga cggcgtgtac gaggtgattc
     3001 cgtgcgacca ggcgcagccg ggcttcaccg cgccgtttcg cgcgcgcgag catttcgacc
     3061 ggctcgaccg cagcctaggc caggtccgga tttccaaccc gctgtcgcgc gaccactggc
     3121 tgcaactggt tgcgcggctg atcgacctgc acccgtggcc gcggcagtcg atctacctgc
     3181 aggtcacgcg cggcgtggcc aaacgcgacc acgtgtttcc cgccggcgtg acgccgaccg
     3241 tgttcgcggt gtcgtcgccg tggccgg
//
LOCUS       DBNHBY010000396         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1648052, whole genome shotgun sequence.
ACCESSION   DBNHBY010000396 DBNHBY010000000
VERSION     DBNHBY010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1648052"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="AC16HB_09860"
     CDS             complement(<1..483)
                     /locus_tag="AC16HB_09860"
                     /inference="COORDINATES: protein motif:HMM:NF039473.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HIR5607134.1"
                     /translation="MHVSVHIRAAARRVAGSLKAAPSRADVLLREADTMLAPLARLLV
                     AHGVTYPRLAQALKAVFLQAAQAELAAAGRTPTDSALSLLSGVHRKDVRALARGAERT
                     GDSGVRALSFAAEVFTRWANDRRYADAQGRPAPLPLHGRGARTFDRLVRSVSKDFHPR
                     S"
     gene            654..1850
                     /locus_tag="AC16HB_09865"
     CDS             654..1850
                     /locus_tag="AC16HB_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR5607135.1"
                     /translation="MNAMFVRIARIAAASGAALTLAACGGGAGGSSTAQGMGTLRVSL
                     TDAPGCGFDAVNVTVERVRVHQSAGAGDADAGWSDIVLASPRRVDLLTLTNGVLLELG
                     QIPLPAGQYQQIRLVLSSNSGAGSPANAVTPTNGSETPTRTPSATQSGLKLVSPFTVQ
                     AGQLVDVVLDFDACKSIVREGNGQYLLKPVIQMIPVIVSGTVTGQLTTTVSGTAVTQA
                     NVSLQKDGTVLRATAPVAGGAYTLAPVDARQQPYDLVITMSGAATSVVSGVPVTANST
                     TNVPPITIGTASAMSTASGTASPAAALPASVRALQAVGAVPKVEIASTNTDGAGAYSL
                     SLPRAASQLATYATPTLSFSAAAISAGKYTLEAKDVTGTKVLTLPPPPTDVNGGNVGN
                     LNFVFP"
BASE COUNT          310 a          660 c          700 g          266 t
ORIGIN      
        1 ggagcgcggg tggaaatcct tcgacaccga acgaaccagg cggtcgaagg tgcgcgcgcc
       61 gcggccgtgc agcggcagcg gagccggccg gccctgcgcg tccgcgtagc ggcggtcgtt
      121 ggcccagcgg gtgaagacct cggccgcgaa cgaaagcgcg cgcacgccgc tgtcgcccgt
      181 gcgctcagcg ccacgcgcca gcgcgcgcac gtccttgcgg tgcacgcccg acagcaggct
      241 cagcgccgag tcggtcggcg tccggcccgc agcggcaagc tcggcctgcg cggcctgcag
      301 gaagaccgcc ttgagcgcct gcgccagccg cggataagtg acgccgtggg cgacgagcag
      361 gcgcgccagc ggcgccagca tcgtgtccgc ctcgcgcagc aggacgtcgg cccgcgacgg
      421 cgcggccttc aaggacccgg ccacgcggcg tgcggccgcg cggatatgaa cagaaacatg
      481 catgggattg tttcccatgc cggcctgcgg cgcaagccgg cccgcgcgcc ggtcagccgg
      541 caatgccaca aatggagtaa tccgcggggg cgtttgaccg cgcggcgatt cgagtgggaa
      601 aatttcccat accatatatg aatgcggacc cggtccgacg ccgaggaggt gccatgaatg
      661 ccatgtttgt ccgaattgcc cggattgccg cggccagcgg cgctgcgctg acgctcgccg
      721 cctgcggcgg cggtgccggc ggctcgtcga ccgcgcaggg gatgggcacg ctgcgggttt
      781 ctttgaccga cgcgccgggg tgcggtttcg acgccgtgaa cgtgaccgtc gagcgcgtgc
      841 gcgtgcacca gagcgcgggt gccggtgacg ccgatgccgg atggagcgac atcgtgctcg
      901 cttcaccgcg ccgggtcgat ctgctgaccc tgaccaacgg cgtgctgttg gagcttggcc
      961 agatcccctt gccggccggg cagtaccagc agatccggct ggtgctgtcg agcaatagcg
     1021 gcgcggggag cccggcgaat gcggtgacac cgaccaacgg cagcgagacg ccgacgagga
     1081 cgccgagcgc gacgcagtcg gggctgaagc tcgtctcgcc gttcacggtg caggcggggc
     1141 agctggtcga cgtcgtgctc gacttcgacg cgtgcaagtc gatcgtgcgg gagggcaacg
     1201 gccagtacct gctgaagccc gtgatccaga tgattccggt gatcgtgtcc ggcacggtca
     1261 cgggacaact gacgacgacg gtttccggca ccgcggtcac gcaggcgaac gtgtcgctgc
     1321 agaaggacgg cacggtgctg cgcgcgacgg cgccggtcgc cggcggtgcc tacacgctgg
     1381 cgccggtcga tgcgcgccag cagccctatg acctcgtgat cacgatgtcg ggcgcggcga
     1441 cctcggtcgt ctccggcgtg ccggtcaccg cgaacagcac caccaacgtg ccgccgatca
     1501 cgatcgggac ggcctcggcg atgagcacgg catcgggcac cgcgagcccg gcggctgcgc
     1561 tgccggcgtc ggtgcgcgcg ctgcaggcgg taggcgcagt gccgaaggtc gaaatcgcga
     1621 gcaccaatac cgacggggcc ggcgcctaca gcctgtcgtt gccgcgcgcg gcatcacagc
     1681 tggcgacata cgcaacgcca acgctgtcgt tctcggctgc cgcgatttcc gcgggcaagt
     1741 acacgctcga agcgaaagac gtcaccggca cgaaggtcct gacgctgccg cccccgccca
     1801 ctgacgtgaa cggcggcaac gtcggcaacc tgaatttcgt gttcccgtaa cgccgagcgg
     1861 cgcagcgcgg atatcatcgt cgctcccgaa cgaggagcga cgatgaaccg actgctcgcg
     1921 atcctgtgcc tggccg
//
LOCUS       DBNHBY010000397         3451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1242245, whole genome shotgun sequence.
ACCESSION   DBNHBY010000397 DBNHBY010000000
VERSION     DBNHBY010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1242245"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..2531)
                     /locus_tag="AC16HB_09870"
     CDS             complement(180..2531)
                     /locus_tag="AC16HB_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HIR5607136.1"
                     /translation="MNTPARQLSQSHDVAAKVAQILQGRTKRASERFSERVQKAGERQ
                     FAASTANPLSLWQSWATTAQYAVDFAQRSILLLDTLRQRGNNFIEHERQGLPPVLRFD
                     YEIVMDGRTLERPVNYALLRIVPPKGVTIDPKRRPFVIIDPRGGHGPGIGGFKDDSQV
                     GVALQEGHPVYFVIFFPNPEPGQTLLDVCNAERDFVHRVRELHPDAPKPGLVGNCQAG
                     WAAMMLAAAHPDDTGPVVIVGAPMSYWGGAWSENEGDNPMRYAGGLLGGTWLASLTSD
                     LGAGKFDGAWLVQNFENLNPANTFWDKYYRVFADIDTEPARFLEFERWWGGYYLMNRE
                     EIEWITQNLFVGNQLWSGTAQGGPAGAFDLRQIKVPIVMFASMGDNITPPQQAFNWVA
                     DVYGSTEEIKARGQVIVGLMQEEVGHLGIFVSGKIAKKEGTQIASVLKSIEALPPGLY
                     GMQIAEKKGADGKPSYEVNLVELRLEEVATRLNRFKRVDEKPFKAAKVVAEFNQRAYE
                     LFGRPLVKTFANEYGAKLSREFHPLRFQRWAFSDLNPFLGWLAPAAQAVKAQRQALAS
                     DHPARQIESTVSEVTSASLDCYRALRDAVAEATFFQVYGNLFAFYLGDERDSETVAPA
                     VDARGLPFVKQALASIDKGGYPEALARVAFLMAHQDEPLPLARVQLASDLLEDYRDML
                     PDLPRDEARRIGGEQEIIARYEPDKAVETLPVLLAKRKDRDRLLTLLERVLRDKRVQR
                     IQPSPEQTAMLARIRRVLGLTGHRPAPAPSRRRIEREAATEEG"
     gene            complement(2528..2764)
                     /locus_tag="AC16HB_09875"
     CDS             complement(2528..2764)
                     /locus_tag="AC16HB_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607137.1"
                     /translation="MALKSESTYPSRRAYVLKLRSDASAGALAGRLENLVTGGQREFA
                     SGHELLASIASDLQASAAERSAEAANINGQRKTS"
     gene            complement(2858..3418)
                     /locus_tag="AC16HB_09880"
     CDS             complement(2858..3418)
                     /locus_tag="AC16HB_09880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607138.1"
                     /translation="MTNDGEQAMSINDGSSVQGHRGSLRGIRIRAVRPDDSERIVKAF
                     RQLDPASIYRRFFAPKKALSGEELRRLTQSDGVRNVVLVATVASGNQEIIVGLGDYAR
                     NGTSAEIAFAVEEDYWARGIASELLRQLTGIARRNGVLRFEADVLPDNAAMLKVFRRS
                     GLPIEATQADGIVHLTLFLDDGSGSA"
BASE COUNT          560 a         1135 c         1090 g          666 t
ORIGIN      
        1 gcgatcgaat tgtcggttcc gcacaccgag gcgtctttct gcagcacgcg ctgaatgacg
       61 ccgggttcga ggatcctgat ctcgcacagc atcgccagcc gcgcaatttc gccatcgagc
      121 tctgcgaggt tgttctccca gaacgcgagt ggaaggtcat gggtctgctc tgtcatggat
      181 catccttctt ctgttgcagc ttcgcgctcg attcggcgcc gcgacggggc cggtgccggc
      241 cggtggcctg tcaaaccaag cacgcgccgg atgcgcgcga gcatcgcggt ctgctctgga
      301 gagggctgga tgcgttgcac ccgcttgtcc cggagcacgc gctcgagcag cgtgaggagg
      361 cggtcgcggt ccttgcgctt ggccaacagc acgggcagcg tctcgaccgc cttgtccggc
      421 tcgtagcgcg cgatgatctc ctgctcgcca ccgatgcggc gcgcttcgtc gcggggcaga
      481 tccggcaaca tgtcccgata gtcctccagc agatcgctcg ccaactgcac tctggcaagc
      541 ggcagcggct cgtcctggtg cgccatcagg aaggcaacgc gcgcgagcgc ttccgggtag
      601 ccccccttgt cgatcgaggc cagcgcttgc ttgacgaatg ggagcccgcg tgcgtccact
      661 gccggcgcca ccgtttcaga atcgcgttcg tcgcccagat agaaggcgaa gaggttgccg
      721 tagacctgga agaaggttgc ttccgccact gcgtcgcgca gcgcccgata acaatcgagc
      781 gaggcgctcg tcacttccga caccgtcgac tcgatctggc gcgctggatg gtctgacgcc
      841 agcgcctggc gctgggcctt caccgcctgc gccgccggcg ccagccaacc gaggaacggg
      901 ttgaggtcgg aaaatgccca gcgctgaaag cggagcgggt gaaactcgcg cgacagcttg
      961 gcgccgtact cgttggcgaa cgtcttgacc agcggccgcc caaagagctc gtaggcgcgc
     1021 tgattgaatt ccgcgacgac cttggccgcc ttgaagggtt tctcgtcgac acgtttgaag
     1081 cggttcaggc gcgtggcgac ttcttccaga cgcaactcaa ccaggttgac ctcgtaggac
     1141 ggcttgccgt cggcgccctt tttctccgcg atctgcatcc cgtacaggcc cgggggcagc
     1201 gcctcgatcg acttgagcac cgaagcgatc tgcgttccct ccttcttggc gatcttgccg
     1261 gagacgaaga tgccgaggtg gccgacttcc tcctgcatca ggccgacgat cacctggccg
     1321 cgcgccttga tttcctcggt gctgccgtac acgtcggcga cccagttgaa ggcctgctgg
     1381 ggcggcgtga tgttgtcgcc catcgacgcg aacatcacga tcggcacctt gatctggcgc
     1441 aggtcgaatg cgccggccgg gccgccttgc gccgtccccg accacagctg gttgccgacg
     1501 aaaagattct gcgtgatcca ctcgatctcc tcgcggttca tcaggtagta gccgccccac
     1561 cagcgctcga actcgagaaa gcgcgccggc tcggtgtcga tgtcggcgaa caccctgtag
     1621 tacttgtccc agaaggtgtt ggccggattc aggttttcga agttctgcac cagccaggcg
     1681 ccgtcgaact tgcccgcgcc caggtcggac gtcagcgatg cgagccaggt gccgccgagc
     1741 agcccgcccg cgtaacgcat cgggttgtcg ccctcgttct cgctccaggc gccgccccag
     1801 taggacatgg gcgcgccgac gatcacgacc gggccggtgt cgtcgggatg ggcggccgcg
     1861 agcatcatcg cggcccagcc cgcctggcag ttgccgacga gtccgggctt cggggcgtcg
     1921 gggtgaagct cgcgcacgcg atgaacgaag tcgcgctcgg cgttgcacac gtcaagcagc
     1981 gtctgtcccg gttcgggatt cgggaagaag atcacgaagt acacgggatg gccttcctgc
     2041 aacgccaccc cgacctgcga atcgtccttg aagccgccga tgccggggcc gtggccgccg
     2101 cgcgggtcga tgatcacgaa gggtcggcgc ttgggatcga tcgtcacccc cttcggcgga
     2161 acgatgcgca gcagcgcgta gttgaccggc cgctcgagcg tgcgcccgtc catcacgatc
     2221 tcgtagtcga agcgcagcac cggcggcagt ccctgccgct cgtgttcgat gaagttgttg
     2281 ccgcgctggc gcagcgtgtc cagcagcagg atcgaacgct gcgcgaagtc aaccgcgtac
     2341 tgcgctgtcg tcgcccagga ttgccacaaa gacagcggat tcgccgtcga cgcagcgaac
     2401 tgccgctcgc ccgctttctg cacccgctca ctgaagcgct ccgacgcgcg cttggtgcgg
     2461 ccttgcagga tctgcgcgac tttcgccgcc acatcgtggc tttgggagag ttgtctggct
     2521 ggcgtgttca tgaggtcttc ctttgtccat tgatattcgc cgcttcggcg gatcgctcgg
     2581 ccgcgctggc ctgcagatcg ctggcgatcg atgcgagcag ttcatggccg gacgcgaatt
     2641 cgcgctgccc gcccgtgacc aggttctcga gacggccggc aagtgcgccg gcactggcat
     2701 cgctgcgcaa cttcagtacg taggcgcgcc ggctggggta ggtggattcg gacttcagtg
     2761 ccattgcagt ctcagtcgaa aaggtagtcg ctttgcatcg ttaagtggtg acagaaccgt
     2821 cgccgttcgg tcggaatgca gaattcgatc cagaccatca ggcgcttccg gagccgtcgt
     2881 cgagaaacag tgtcaagtga acgatgccat cggcttgagt tgcttcgatc ggcaatccgc
     2941 tgcgccggaa taccttcagc atcgccgcgt tgtcgggaag cacgtccgcc tcgaaccgca
     3001 ggacaccgtt gcgacgagcg atgcctgtca gttgccgaag cagttcactt gcgattccgc
     3061 gggcccagta atcctcttcc accgcgaacg caatctctgc gctcgttcca ttccgcgcgt
     3121 agtcacccag accaacgatg atttcctgat tgccgcttgc gaccgtggca actagcacaa
     3181 cgttgcgtac gccgtcgctc tgggtgagcc gccgcagttc ctctccgctc agcgccttct
     3241 tgggagcgaa gaaccgccgg tagattgatg cagggtccag ttgccgaaat gctttgacga
     3301 tgcgttcgct gtcgtcaggg cggacggcgc gaatgcgtat gcccctcaaa ctcccacggt
     3361 ggccctgcac gctcgagccg tcattgatac tcatcgcttg ctcgccgtcg ttcgtcatct
     3421 gcgctccggc tcgctagctc cgccatctcg t
//
LOCUS       DBNHBY010000398         2330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1512561, whole genome shotgun sequence.
ACCESSION   DBNHBY010000398 DBNHBY010000000
VERSION     DBNHBY010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1512561"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC16HB_09885"
     CDS             <1..632
                     /locus_tag="AC16HB_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HIR5607139.1"
                     /translation="GAAAVRHAAESRTEDAAAAAMTPKDVLDFWFGAPGEPDAGRERE
                     FWFRKSTATDEMIRARFGADVEAALRGERDDWTGTVDGTRALILLLDQFTRNIFRDTA
                     RAFAGDARALELARGLVASGADRTLAPLQRWFVYLPFEHSESMAAQHESLRLFGELAA
                     AGFADGLVWAQKHHDVIARFGRYPHRNAILGRTSTPGELDYLAQPGAGF"
     gene            complement(639..1130)
                     /locus_tag="AC16HB_09890"
     CDS             complement(639..1130)
                     /locus_tag="AC16HB_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5607140.1"
                     /translation="MDPIKRIRSEVERGLVRAWDSLADGWRELSSRAGGALTHYVRRA
                     RRGRSVEQDFPAWALLAAEVWETALSVIVRVELPGMSKEDIDISVSGTRLRMRGEKRS
                     AGEHKGRRYHLMERAFGSFEREFSLPHSVDSSRAEVSYQDGVVTVILPKTEATPPQRL
                     PIK"
     gene            1264..1704
                     /locus_tag="AC16HB_09895"
     CDS             1264..1704
                     /locus_tag="AC16HB_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5607141.1"
                     /translation="MFGSQANFESLYDELRRMEQEMDQMFGRWSAPAGIRWVSRGTFP
                     AANVGATGDAAHVYLFAPGVDPKAIELTLQENLLLVSGKRQVVADKKSTYYRQERFSG
                     EFRRVITLPDDVDPERVDANYRDGVLQITLKRRESARPRKIEVH"
     gene            1724..2119
                     /locus_tag="AC16HB_09900"
     CDS             1724..2119
                     /locus_tag="AC16HB_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5607142.1"
                     /translation="MSDNVATRPESAVAPTGRREDETYLRPPADIFEDAEGISVQLDL
                     PGVSKDRLNVHADPNTLVIEGEVMIDLPQDMEALHAEVQSTHYYRSFALSGELDTEKT
                     QANLKDGVLTVTIPKRAELKPRKIQISAA"
BASE COUNT          415 a          772 c          790 g          353 t
ORIGIN      
        1 ccggcgctgc cgcagttcgg catgcggccg aatctcgcac cgaagacgct gccgccgcgg
       61 cgatgacacc gaaggacgtg ctcgacttct ggttcggcgc gccgggggag cccgacgcgg
      121 gccgcgagcg tgaattctgg tttcgcaagt cgacggcgac cgacgagatg atccgcgcgc
      181 gcttcggcgc cgacgtcgaa gccgcgctgc gcggcgagcg cgacgactgg acggggacgg
      241 tcgacggcac gcgcgcgctg atcctgctgc tcgatcagtt cacgcgcaac atcttccgcg
      301 acacggcgcg cgcgtttgcc ggcgatgcgc gcgcgctcga actcgcgcgc gggctggtcg
      361 cgtccggcgc cgaccggacg ctcgcgccgc tgcagcgctg gtttgtctac ctgccgttcg
      421 agcactcgga gtcgatggca gcgcaacacg agtcgctgcg cctgttcggc gagcttgcgg
      481 ccgccggttt cgccgacggg ctggtgtggg cgcagaagca ccatgacgtg attgcgcggt
      541 tcggccgcta tccacaccgc aatgcgatcc tcggccggac gtcgacgccc ggggagctgg
      601 actacctcgc gcagcccggc gccgggttct gacggcagct acttgatcgg cagccgctgc
      661 ggcggcgtgg cctcggtctt cggcaggatc acggtcacca cgccatcctg gtaggagact
      721 tcggcacgac tgctgtcgac gctgtgcggc aggctgaact cgcgctcgaa gctgccgaat
      781 gcacgctcca tcaagtggta gcggcgaccc ttatgctcgc cggccgagcg cttctccccg
      841 cgcatgcgca gcctggtccc gctgaccgag atatcgatgt cctccttgga catgccgggc
      901 agttccaccc gaacgatcac cgacagcgcg gtctcccaca cctcggcggc cagcagcgcc
      961 cacgcgggga agtcctgctc cacgctccgg cccctgcgtg cgcggcgcac atagtgcgta
     1021 agcgcgccac cggcgcggct cgacagttcg cgccagccat ccgcaaggct gtcccaggca
     1081 cgaacgaggc cgcgctcgac ctcagagcgg atgcgtttga tcggatccat cgtacgtccc
     1141 cgaaagtggg cgccgccggc gccgattcaa gtcccgccct tgccgccaag cttgaaatcg
     1201 gcacggcgaa tccatagcta aaccggtgca atccggttca accacctgaa aggaggtact
     1261 gagatgttcg gaagtcaggc gaacttcgaa agtctctacg acgaactgag gcggatggag
     1321 caggagatgg accagatgtt cggccgctgg tcggcgccgg cggggatccg ctgggtctcg
     1381 cgcgggacat ttccggccgc caacgtcggc gcgacgggcg acgcggccca cgtctacctg
     1441 ttcgcaccgg gcgtcgatcc gaaggcgatc gagctgacgc tgcaggagaa cctgctgctc
     1501 gtctccggca agcggcaggt agtcgcggac aagaagtcga cttactaccg gcaggagcgt
     1561 ttcagcggcg agttccggcg cgtgatcacg ctccccgacg acgtcgatcc ggaacgtgtc
     1621 gacgccaact accgcgacgg cgtcctgcag atcacgttga agcggcgcga atccgcgcgc
     1681 ccgcgcaaga tcgaagtcca ctgaatgccg aggagatgaa accatgagcg acaacgttgc
     1741 gacccgcccg gaatccgcgg tggcgccgac cggcaggcgc gaagacgaga cctatctgcg
     1801 gccgccggcc gacatcttcg aggatgccga aggcatctcg gtgcagctcg acctgcccgg
     1861 cgtttcgaag gatcgcctga acgtgcatgc cgatccgaac acgctcgtga tcgaaggtga
     1921 agtgatgatc gacctgccgc aggacatgga ggcgctgcac gcggaggtgc agtcgacgca
     1981 ctattaccgc agcttcgcgc tgagcggcga gctggacacc gagaagacgc aggcgaacct
     2041 gaaggacggc gtactgaccg tgacgattcc caagcgcgct gagctgaagc cgcgcaagat
     2101 ccagatcagc gccgcgtaag cgccggaggg atcggaacaa tcgatcgcgg catcgctcga
     2161 ggccagtgca gcttctccga ggcaaaggag gtgaagacaa tgacacggat gaagcattgg
     2221 caggacgtgg tgggcgccgt accgggcgcc tggctgatcg tcgcgatcct cgcgctgtcg
     2281 gtgctgctga ccgacaagga ctacagcggc tggtggcgca aggccgcgca
//
LOCUS       DBNHBY010000399         7841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_362512, whole genome shotgun sequence.
ACCESSION   DBNHBY010000399 DBNHBY010000000
VERSION     DBNHBY010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_362512"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /gene="lepB"
                     /locus_tag="AC16HB_09905"
     CDS             <1..478
                     /gene="lepB"
                     /locus_tag="AC16HB_09905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5607143.1"
                     /translation="DVMVFRFPHNPSQDYIKRVIGLPGDRVEYLDRRLTTNGKPVETV
                     GVGRYLEEGRLQYYAEYTEKLGQLSHRIILSESPMGVPIMSTGQHTNPEACRYQPDGR
                     GVTCVVPPGHYFMMGDNRDNSEDSRFWGFVPDENIVGRAFFIWMNFGNISRIGSFN"
     gene            503..880
                     /locus_tag="AC16HB_09910"
     CDS             503..880
                     /locus_tag="AC16HB_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR5607144.1"
                     /translation="MYMRKPGQRQRGLSLIGLLFVGLIVVFLLVIGFKVVPAVTEYIA
                     IEKAIRKINKDGATVPQIRREFENYVIIDDIKSIAGKDLDITKENDRVVISYAYSYPI
                     PIMDNVRLVIDFSGSTRTRSKTP"
     gene            905..1714
                     /gene="rnc"
                     /locus_tag="AC16HB_09915"
     CDS             905..1714
                     /gene="rnc"
                     /locus_tag="AC16HB_09915"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR5607145.1"
                     /translation="MTLAALEQRLGYTFQNPALLEQAVTHRSHGAVHNERLEFLGDAV
                     LNCTIAQLLYQKYARLNEGDLSRLRANLVKQQSLADIGEKLGLSDFLRLGEGEMKSGG
                     FRRPSILADTMEAIYGAVLVDGGFEAARDTIARLFEPVLKTVDPKTLGKDSKTLLQEY
                     LQGKRLPLPVYTVVETRGAAHNQEFAVECGIPKLEISVRGTGRSRRAAEQSAAKQALD
                     QAHAVVQAARRAKRKSAESATEAGQPAPPPVEAAADGTRNALAAQTAAEKP"
     gene            1714..2619
                     /gene="era"
                     /locus_tag="AC16HB_09920"
     CDS             1714..2619
                     /gene="era"
                     /locus_tag="AC16HB_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR5607146.1"
                     /translation="MSEAPDPVRPRTGFVAIIGRPNVGKSTLLNALVGEHVSITSRKP
                     QTTRQRVVGVLTRDSTQFIFVDTPGFQQRHKSRLTRRMNEEVSAALADVDAIVLVIEA
                     RGWTAEDERLLRLIPAERRNVVLAINKTDLLADRDGLLPLLAQSVQRFSFAAMVPVSA
                     ERGRQLDDLLQEVERLLPEGEPLFGADQFTDRSARFLAAEFVREKAFRLLGDELPYGV
                     AVTIEQWDEDERRADIAASLWVARDSHRSIVIGAGGAKLREIGRLARGEIERMLGKPV
                     HLQTHVRVKRGWTDGARALKGLGYE"
     gene            2612..3346
                     /gene="recO"
                     /locus_tag="AC16HB_09925"
     CDS             2612..3346
                     /gene="recO"
                     /locus_tag="AC16HB_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5607147.1"
                     /translation="MNSAARPGSDARVDHAPAYLLHAYAWRETSLIAEMFTRDHGRVA
                     LVARGAKRPTSHFRGVLTSFCPLLIGWSGRNEVKTLVRAEWCGGLAPLRGEAVLAGFY
                     MNELLVRLLARGDAHEQLFAAYGQALADLARAAQPAATLRSFELALLREAGFLPALDH
                     CEDGAPIQPGARYRIESARGLVRVDDGGDELTIDGATALAMARGDFGDARVAGESKAA
                     LRQLIRYHLDGRPLNTRRILQDLKEL"
     gene            3343..4113
                     /locus_tag="AC16HB_09930"
     CDS             3343..4113
                     /locus_tag="AC16HB_09930"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR5607148.1"
                     /translation="MTALSVNLNKVALVRNTRALGIPSVQRAATLALEAGADGVTVHP
                     RPDGRHVRGDDVRQLAALLKGWPACEFNIEGNPFHQLLDYVREVRPHQCTFVPDESGA
                     FTSDHGWDLPRDSGRLRPVIEEARALGVRVSLFMDPLPEAMQLARELGADRVELYTEP
                     YAKAYGTAQQDEELARYRRAAEAAQRVGLGVNAGHDLNRANLAAFLIAVPGVDEVSIG
                     HALIADALEFGLADTVRKYLAEIRRAGAAAPYALRDGR"
     gene            4110..4517
                     /gene="acpS"
                     /locus_tag="AC16HB_09935"
     CDS             4110..4517
                     /gene="acpS"
                     /locus_tag="AC16HB_09935"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5607149.1"
                     /translation="MIYGIGIDLIEIERIAQAYARHGERFSQRILGPDEWQRYDGRRQ
                     RSAARGIAYLATRFAAKEAVSKALGLGIRSPMTWRAAQILNAPSGRPEVVASGALAEF
                     IRARRLRLRVSVTDERTLAAAFVIAETVEEDER"
     gene            4514..5530
                     /gene="nagZ"
                     /locus_tag="AC16HB_09940"
     CDS             4514..5530
                     /gene="nagZ"
                     /locus_tag="AC16HB_09940"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR5607150.1"
                     /translation="MIGPVVVDVAGPRLTANERARLAHPLVGMVILFKRNYESPAQLR
                     ALCEEIHSLRTPPLLIAVDHEGGRVQRFRGAPFTAIPAMDALGRLWDHDVLQACRVAV
                     SAGYVMGAELRAHGVDLTFAPVLDLAWSRSSVIGDRAFHHDARVVAMLANHLCHGLSL
                     AGMANCGKHFPGHGWAHADSHVAVPVDERSMQTIVDADAAPYRWLGASLASVMPAHVI
                     YPAVDSAPAGFSRRWLRQILRDEFGFTGAVFSDDLSMEGAKVVGAMVARAQAALDAGC
                     DFVLVCNDSEAADQVLGGARWRRDAGFDERVQRLAPRGPALDVDSLQASTLYRAARKD
                     LESI"
     gene            complement(5582..6148)
                     /gene="efp"
                     /locus_tag="AC16HB_09945"
     CDS             complement(5582..6148)
                     /gene="efp"
                     /locus_tag="AC16HB_09945"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5607151.1"
                     /translation="MAKILATEIRAGNLIEWDKRLWRVLKQYHVHVGGRGGAYMQVEM
                     KDIETGQKRNERLATDEKIERPYIDKRRMTYSYQDGSTYVFMDNETYEQLSLSAEFLE
                     GQSGYLLPNMEVEINFHNGRALGVELPGSVVLEVVETEPAIKNATATTTFKPAKMETG
                     ITVQVPPFVNTGDRIRVNTDDGAYIERA"
     gene            6231..>7841
                     /gene="uvrC"
                     /locus_tag="AC16HB_09950"
     CDS             6231..>7841
                     /gene="uvrC"
                     /locus_tag="AC16HB_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR5607152.1"
                     /translation="MRAVPQPSVDFDPKAVIERLPHLPGVYRMLDADGGTLYVGKARD
                     LKKRVASYFIKGVGPRTELMLSRVANIETTVTRSEAEALLLENNLIKSLAPKFNILFR
                     DDKSYPFLKLTDHAFPRVAYYRGATDKRARYFGPFPNAWAVKDSIQVLQKVFRLRTCE
                     DTVFAHRSRPCLLHQIQRCSAPCVGQIDAAAYAQDVDRAVRFLRGQTDEVMAELESRM
                     QSFAAELQFERAAAVRDQLGALSRVLHQQAIETTGGDSDADILAVVSEGGQACVNLAM
                     VRGNRHLGDHAYFPLRTDTGVEDAEVLDAFISQHYIGQSVPPVLIVNVELDAAELQQM
                     LAGEGGTVQIVSGSDGGLRAQRRRWLEMAQANARLALARRLAEEGSQQARTRALLEAL
                     AIELPDPDPATLRVECFDVSHTQGEATQSSCVVYHHHAMQPSEYRRFNIEGIEPGDDY
                     AAMRQTLTRRYAPAARGEAPLPHLVLVDGGAGQVSVAVQAFEELGLDSSIIVGVAKGE
                     ERKTGLEELIFADGRASLALGRESQALML"
BASE COUNT         1273 a         2576 c         2745 g         1247 t
ORIGIN      
        1 cgacgtgatg gtgttccgct ttccgcacaa tccctcgcag gactacatca agcgcgtgat
       61 cgggctgccg ggcgaccggg tcgagtacct cgaccgtcgc ctcaccacca acggcaagcc
      121 ggtcgagacc gtcggcgtcg gccgctacct agaagaaggg cgactgcagt actacgcgga
      181 atacacggaa aaactgggcc agctcagcca ccggatcatc ctgagcgaaa gcccgatggg
      241 cgtaccgatc atgtcgaccg ggcagcacac gaatccggag gcgtgccgct atcagccgga
      301 cggccgaggc gtgacctgcg tggtgccgcc cgggcactac ttcatgatgg gtgacaatcg
      361 cgacaacagc gaggacagcc gcttctgggg cttcgtgccc gacgagaaca tcgtggggcg
      421 cgcctttttc atctggatga attttggcaa tatcagtcgc atcggcagct tcaactgaca
      481 gtcgcgagac agcggagacg acatgtacat gcgcaaaccc gggcagcggc agcgtggact
      541 gagcctgatc gggctcctgt tcgtcggcct gatcgtcgtt ttcctgctgg taatcggctt
      601 caaggtcgtt ccggcggtca ccgagtacat cgccatcgag aaggcgattc gcaagatcaa
      661 caaggacggc gccacggtgc cgcagatccg gcgcgaattc gagaactacg tgatcatcga
      721 cgacatcaag tcgatcgcgg gcaaggatct cgacatcacc aaggagaacg accgcgtggt
      781 gatctcgtac gcgtactcgt atccgatccc gatcatggac aacgtgcggc tggtgatcga
      841 tttctccggc tcgactcgca cgcgcagcaa gacgccctga ccgtggcgcg caaggactgg
      901 tggcatgact ctggcggcgc ttgagcagcg cctgggatac acgttccaga atccggccct
      961 gctggagcag gcggtaacgc accgcagcca cggcgcagtc cacaacgagc ggctcgaatt
     1021 cctcggcgat gcggttctga actgcaccat cgcgcaactg ctgtatcaga agtacgcgcg
     1081 gttgaacgag ggcgacctgt cgcgcctgcg cgcaaacctc gtcaagcagc agtcgctggc
     1141 ggacattggc gaaaaactcg ggctctcgga cttcctgcgc ctgggcgaag gcgagatgaa
     1201 gagcggcggc tttcgccgcc catcgatcct cgccgacacg atggaggcga tctacggcgc
     1261 ggtactggtc gacggcggct tcgaggccgc gcgtgacacg atcgcgcggc tgttcgagcc
     1321 ggtgctcaag accgtcgacc cgaagacgct cggcaaggac agcaagacgc tgctgcagga
     1381 atacctgcag ggcaagcgcc tgccgctgcc cgtatacacc gtggtcgaga cgcgcggcgc
     1441 ggcgcacaac caggaattcg cagtcgagtg cggaatcccg aagctcgaga tcagcgttcg
     1501 cggcaccggg cgcagccgac gcgccgccga gcagtctgcc gccaagcagg ccctcgacca
     1561 ggcgcacgcg gtcgtgcagg ccgcgcggcg cgccaagcgg aagagcgccg agtcggccac
     1621 cgaggcgggc caacctgcgc cgccgccggt ggaagccgcg gccgacggga cgcgcaatgc
     1681 gctcgccgcg cagacggccg cggagaaacc gtgatgtccg aggcgcccga ccccgtgcga
     1741 ccgcgcacgg gctttgtcgc gatcatcggg cggccgaacg tcggcaagtc gacgttgctc
     1801 aacgcgctgg tcggcgaaca cgtatcgatc acgtcgcgca aaccgcagac gacccggcag
     1861 cgggtggtgg gcgtgctgac tcgcgactcg acgcagttca tattcgtcga cacgccgggc
     1921 tttcagcagc gccacaaatc gcggctgacg cgacggatga acgaggaggt gtccgcggcg
     1981 ctcgctgacg tcgacgcgat cgtgctggtg atcgaggcca ggggctggac ggcggaggac
     2041 gagcgtctgc tgcggctcat ccccgccgag cgccgcaacg tcgtgctcgc gatcaacaag
     2101 accgacctgc tggccgatcg cgacgggctg ctgccgctgc tggcgcaatc ggtgcagcgg
     2161 ttctccttcg cggcgatggt gccggtcagc gccgagcgcg ggaggcagct cgacgacttg
     2221 ttgcaggaag tcgagcggct tctgccggaa ggcgaaccgt tgttcggcgc cgaccagttc
     2281 accgaccgca gcgcgcgctt cctcgccgcg gagttcgtgc gcgagaaggc gtttcgcctg
     2341 ctgggcgacg agttgccgta cggagtcgcc gtgacgatcg agcagtggga cgaggacgag
     2401 cgccgcgcgg acatcgccgc gtcgctgtgg gtcgcgcgcg attcgcatcg atcgattgtc
     2461 atcggggcgg gcggcgccaa gctgcgcgag atcgggcgcc tggcgcgcgg cgagatcgag
     2521 cgcatgctcg gcaagccggt gcatctgcag acgcacgtgc gcgtgaagcg cggctggacc
     2581 gacggcgcgc gcgcactaaa gggcctcggc tatgaatagc gcagcgcggc ccgggtcgga
     2641 cgcgcgcgtc gaccatgcgc cggcctatct cctgcacgcg tacgcctggc gcgagaccag
     2701 cctgatcgcg gagatgttca cgcgcgacca cggtcgcgtg gcactggtgg cgcgcggcgc
     2761 caagcggccg acctcgcatt ttcggggtgt gctgacatcc ttctgcccgc tgctgatcgg
     2821 ctggagcggg cgcaacgagg tcaagacgct cgtgcgcgcc gagtggtgcg gcggactggc
     2881 gccgctgcgc ggcgaggcgg tgctggcggg cttctacatg aacgaactgc tggtgcgcct
     2941 gctggcgcgc ggcgacgcgc acgagcagtt gttcgccgcg tacgggcagg cgctcgccga
     3001 tctcgcacgc gctgcgcagc cggccgcgac actgcgcagc tttgagctcg cgctgctgcg
     3061 cgaggccgga ttcctgccgg cgctcgacca ctgcgaggac ggtgcaccga tccagcccgg
     3121 cgcgagatac cggatcgaat cggcgcgcgg cctggtacgt gtcgacgacg gtggcgacga
     3181 actgacgatc gacggcgcca cggcgctcgc gatggcgcgc ggcgacttcg gtgacgcgcg
     3241 cgtggcgggc gagagcaagg ccgcgttgcg gcagctgatt cgctaccatc tcgacggccg
     3301 tccgctgaac acgcgccgca tcctgcagga cctgaaagag ctgtgaccgc gctctccgtc
     3361 aatctgaaca aggtcgcgct ggtgcgcaac acgcgcgcgc tcggcattcc gagcgtgcag
     3421 cgcgcggcga ccctcgcgct cgaagccggg gcggacggcg tcaccgtgca tccacggccg
     3481 gacggccgcc atgtgcgcgg cgacgacgtg cgccagctcg cggcgctgct gaagggctgg
     3541 cccgcgtgcg aattcaacat cgaaggcaat ccgttccacc agctgctcga ttacgtgcgc
     3601 gaggtgcggc cccaccagtg cacgttcgtg ccggacgaga gcggcgcatt cacgtccgac
     3661 cacggctggg acctgccgcg tgacagcggc cggctgcggc cggtgatcga agaggcgcgg
     3721 gcactcggcg tgcgggtcag cctgttcatg gatccgctgc cggaggcgat gcaattggcg
     3781 cgcgaacttg gcgccgaccg cgtcgagctc tacaccgagc cctacgcgaa agcctacggc
     3841 acggcacagc aggacgagga actggcgcgt taccggcgcg ccgccgaggc ggcgcaacgc
     3901 gtcggactcg gcgtcaatgc ggggcacgac ctgaatcgcg ccaacctcgc ggcgttcctg
     3961 atcgcggttc ccggcgtgga cgaggtctcg atcggccacg cgctgatcgc cgacgcactc
     4021 gagttcggct tggcggacac cgtgcgcaag tatctcgccg agatccgtcg cgccggcgcg
     4081 gcggcaccgt acgcgctgcg cgacggccgg tgatctacgg catcggcatc gatctgatcg
     4141 agatcgagcg catcgcgcag gcatatgcac gccatggtga acgcttctcg cagcgcattc
     4201 tcggtcccga tgaatggcag cgttacgacg ggcgccgcca gcggtcggcg gcgcgcggga
     4261 tcgcctatct cgcaacgcgc ttcgccgcca aggaggcggt ttcgaaggct ctcgggttgg
     4321 gcatccgctc accgatgacc tggcgcgcgg cgcagatcct gaacgccccg tccggccgcc
     4381 cggaggtcgt cgccagcgga gcgctggccg agttcatacg cgcgcggcgg ctgcggctgc
     4441 gggtgtcggt caccgacgag cgcacgctgg cggcggcgtt cgtcatcgcc gaaaccgtag
     4501 aggaggacga gagatgatcg gacccgtcgt ggtcgatgtg gcgggaccgc ggctgacggc
     4561 gaacgagcgt gcgcggctcg cccatcccct cgtcggcatg gtgatcctgt tcaagcggaa
     4621 ctacgaatcg ccggcgcagc tgcgcgcgct gtgcgaggag atccactcgc tgcgcacgcc
     4681 gccgctgctg atcgcggtcg atcacgaggg cgggcgcgtg cagcgtttcc gcggcgcgcc
     4741 gttcaccgcg atcccggcga tggacgcgct cggacgcctg tgggaccacg acgtgctgca
     4801 ggcatgccgg gtcgccgtgt cggcgggcta tgtgatgggc gccgaactgc gcgcgcacgg
     4861 cgtcgacctg acgttcgcac cggtgctcga cctggcgtgg agccgcagtt cggtgatcgg
     4921 cgaccgcgcg ttccatcacg acgcgcgtgt cgtcgcgatg ctggccaacc acctgtgcca
     4981 cggcctgtcg ctggcgggca tggcgaattg cggcaagcat tttcctgggc acggctgggc
     5041 gcacgcggat tcgcatgtcg cggtgccggt cgacgagcgt tcaatgcaga cgattgtcga
     5101 cgccgatgct gcgccgtacc gctggctcgg cgcttcgttg gcgagcgtga tgcccgcgca
     5161 cgtgatctat cccgcagtcg attccgcgcc ggccggcttc tcacgccgct ggctgcggca
     5221 gatcctgcgc gacgaattcg gtttcaccgg cgcggtgttc agcgacgatc tatcgatgga
     5281 gggggcgaag gtggttggcg ccatggttgc gcgcgcgcag gccgcgctcg acgccggctg
     5341 cgacttcgtg ctcgtttgca acgactccga ggcggccgat caggtgctcg gcggcgcgcg
     5401 gtggaggcgc gacgcgggct tcgacgagcg ggtgcagcgg cttgctccgc gcgggcctgc
     5461 gttggatgta gattcgttgc aggcaagcac gctgtatcgc gcggcgcgca aggatctgga
     5521 gtcgatctga ccaggccccc gctgagcgag cagaaggccg cgggtccgtg gtgccgcggc
     5581 gtcaggcgcg ctctatgtag gcgccatcgt ccgtattcac gcgaattcgg tcgcccgtgt
     5641 tcacgaaggg cggcacctgc accgtgatgc cggtttccat cttcgccggc ttgaaagtcg
     5701 tggtggcggt cgcgttcttg atcgctggct cggtctcgac gacctcgagc accacgctgc
     5761 ccggcaactc gacgccaagc gcgcggccgt tgtggaagtt gatctcgact tccatgttcg
     5821 gcagcaggta gccgctctgg ccctcgagga actcggcgct cagcgaaagc tgctcgtacg
     5881 tctcgttgtc catgaagacg taggtcgaac cgtcctggta cgagtacgtc atcctgcgct
     5941 tgtcgatgta cggacgctcg atcttctcat cggtcgccag ccgctcgttg cgtttctggc
     6001 cggtctcgat gtccttcatc tcgacctgca tgtatgcgcc gccgcgcccg ccgacgtgca
     6061 cgtggtactg cttcagcacg cgccacagac gcttgtccca ttcgatcagg ttgccggcgc
     6121 gaatttcggt cgcaagaatc tttgccatgg tgggtccgcg ggcgactcgt aaaatcggag
     6181 ccgcattgaa gtgagcccgc gattatagag gcgcgggcgg attcattcga ctgcgcgccg
     6241 tgccgcaacc ctccgtcgac ttcgacccca aggccgtcat cgaacgcctg ccgcacctgc
     6301 cgggcgtcta ccggatgctc gatgcggacg ggggcacgct ttacgttggc aaggcacgcg
     6361 atctgaagaa gcgcgtcgcc tcgtatttca tcaagggcgt cgggccgcgc accgaactga
     6421 tgctgtcgcg cgtggcgaac atcgagacaa cggtcacgcg ctccgaggcc gaggcgctgc
     6481 tgctcgagaa caacctgatc aagtcactgg cgccgaaatt caacatcctg ttccgtgacg
     6541 acaagtccta tccctttctc aagctgaccg accacgcgtt cccgcgcgtc gcctactacc
     6601 gtggcgcaac cgacaagcgg gcgcgctact tcgggccgtt tccgaacgcg tgggcggtca
     6661 aggattcgat ccaggtgctg cagaaggtgt tccgcctgcg cacctgcgaa gacaccgtct
     6721 tcgcgcaccg ctcgcgcccg tgcctgctgc atcagatcca gcgctgcagc gcgccgtgtg
     6781 tcggccagat cgacgccgca gcgtatgcgc aggacgtcga tcgcgcagtg cggttcctgc
     6841 gtgggcagac cgacgaggtc atggccgagc tcgagtcaag gatgcagtcg ttcgctgccg
     6901 agctgcagtt cgagcgcgcg gccgcagtgc gcgaccagct cggtgcgctg tcgcgggtgc
     6961 tgcaccagca ggcgatcgag acgaccggcg gcgacagcga cgccgacatc ctggcggtcg
     7021 tctccgaagg cgggcaggcg tgcgtgaatc tggcgatggt gcgcggcaac cgccatctcg
     7081 gcgaccatgc gtactttccg ctgcgcacgg acactggcgt cgaggacgcg gaagtgctgg
     7141 acgcattcat ctctcagcac tacatcggcc agagcgtgcc gcccgtgctg atcgtcaacg
     7201 tcgaactcga cgcggccgaa ctgcagcaga tgctggccgg cgagggcggc acggtgcaga
     7261 tcgtgagtgg cagcgacggc ggtctgcgcg cgcagcgccg tcgctggctc gagatggcgc
     7321 aggcgaacgc gaggctcgcg ctggcgcggc ggctggcgga agagggctcg cagcaggcgc
     7381 gcacgcgcgc cctgctcgag gcgctggcga tcgagctgcc ggaccccgac ccggccacgc
     7441 tgcgcgtcga atgcttcgac gtcagccaca cgcaaggcga ggccacacag tcgtcctgcg
     7501 tcgtctatca ccaccacgcg atgcagccgt cggagtaccg gcgcttcaat atcgagggca
     7561 tcgagccggg cgatgactac gcggcaatgc gccagacgct gacgcgccgc tacgcgccgg
     7621 cagcgcgcgg cgaagcgccg ctgccgcacc tggtgctggt cgatggcggc gcggggcagg
     7681 tcagcgtggc ggtgcaggcg ttcgaggagc tcggactcga ttcgtccatc atcgtcggcg
     7741 tggccaaagg ggaggaacgc aagacggggc tggaggaact gatcttcgcc gatggccgtg
     7801 cgtcgctcgc gctcggccgc gaatcgcagg cgctgatgct g
//
LOCUS       DBNHBY010000400         9061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1850459, whole genome shotgun sequence.
ACCESSION   DBNHBY010000400 DBNHBY010000000
VERSION     DBNHBY010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1850459"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16HB_09955"
     CDS             complement(<1..458)
                     /locus_tag="AC16HB_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR5607153.1"
                     /translation="MAASALLAGCKGSSSDGATVTVAGDVALAYAMRANSIRANPTNG
                     APTAPGGDLMIREKSSPSAPEHNITERFTQGQGDVSDPEVSYDGKKIVFAMRCPTANT
                     ATIDNQPACTGRWNIWEYDMTSGGLAGGTFRRITSSTDDDDVDPAYLPAGR"
     gene            complement(529..1521)
                     /locus_tag="AC16HB_09960"
     CDS             complement(529..1521)
                     /locus_tag="AC16HB_09960"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR5607154.1"
                     /translation="MQASASLMPWTARKRDVQNSQHGWFSRDEAIMGTAIRVELWSDD
                     RASAQPAIDAVMQEMHRIDAQMSPYKPDSELSRINRDAAAGPVQISDELFDLLARSIE
                     FSRLSGGAFDITYAAVGHLYDYRRGIRPSEAEIARARTAVGYRNLLLDRRARTVRFAR
                     PGMRIDLGGFAKGYAVDNGAAILRRRGIAHAIVTAGGDSHIVGDRRGRPWTIGIRDPR
                     SAGEVIALLPLEEAAVSTSGDYERCFELDGVRHHHLIDPSTGESPRGVRSVTIVAADG
                     LTSEALSKTVFVLGAARGLQLIESISGVDAVAVDALGQLHFSSGLLAGGNPTRQ"
     gene            complement(1856..3160)
                     /locus_tag="AC16HB_09965"
     CDS             complement(1856..3160)
                     /locus_tag="AC16HB_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR5607155.1"
                     /translation="MASMLFARAQATLVGSIKALLRVDQWGQRFGPWTAQTRDVLRFA
                     LRRAHEVRLAQVAGGLTFTTVLSIVPLLAVALSLFTAFPLFTEFRVSLEKSLFRGLLP
                     EQYAPLILRYLNEFTAKAGRLTAFGLFFLLLTALSMILTVDHIFNDIWQVRTRRPLLQ
                     RVLLYWSLLTLGPLVIGASLSAASYVATASAGWSERVPGALLTVLEYTPFVLGVSAVA
                     AAYMVVPNRRVHWRDAGIGALIAAVLGELMRDGFAWYIRTGTVANIYGAFAVLPLFLI
                     WVYLSWLVILFGAAIAATVPMLRAMRFADESRAGNAFITAVALLRALHRARRRGDNDG
                     RLPLEALARAVRSAPADAERTLMALERLGYVAPLAGAHAGSWLLTCDPARANLVDAFR
                     FFAVDPANTLAARDADGLGAWLDAGLHADWIARPLDEVLRTN"
     gene            3200..3790
                     /gene="wrbA"
                     /locus_tag="AC16HB_09970"
     CDS             3200..3790
                     /gene="wrbA"
                     /locus_tag="AC16HB_09970"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR5607156.1"
                     /translation="MEILVLYYSRHGATRKLAEHVATGIDAVAGAHARLRTVPPVAAV
                     TQTAAKPVPPDGAPFAELRDLEECAALALGSPTRFGNMAAPLKHFLDGTSGHWLAGTL
                     AGKPAALFTSTSSLHGGQESTLLSMMIPLLHHGMLIVGLPYTEPELMTTTTGGGPYGA
                     SHIARQQGAAAISDEERRLAVALGRRLATITVKLRA"
     gene            3787..4167
                     /locus_tag="AC16HB_09975"
     CDS             3787..4167
                     /locus_tag="AC16HB_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HIR5607157.1"
                     /translation="MTTVALTRMQRAAGRIAAASWLALLALAVLWEWLLAPLRPGGSW
                     LILKAVPLLVPLRGVWRGEPYALQWALLLTPFYFCEGVVRTFDAVPVARLAWIEALLA
                     VVFFVAAIVYLRPFKRAARRTGPG"
     gene            4164..5564
                     /locus_tag="AC16HB_09980"
     CDS             4164..5564
                     /locus_tag="AC16HB_09980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5607158.1"
                     /translation="MNLLEQLTTIVGVGNVLIGSDAGPYLTDWRRRYTGAALAVVRPG
                     TAEDVTRVVRACIGMRAPIVPQGGNTGLVGGGTPDATGRAVVVSLARMNRVRALDTAN
                     DAITVEAGCVLQNVQDAARAAQRLFPLSLAAEGSATIGGNLSTNAGGTQVLRYGNARD
                     LTLGLEVVLPSGELWDGLRSLRKDNTGYDLKHLFIGAEGTLGIITAATLKLYPLPAAQ
                     VTVFASASSLHAALALLNRARAAAGPALTAFEVMSDLCLRRFAREFPDVRLPLPLASP
                     WFALLELSDHESAEHAAARLESILGAALEGREVDDAVIAKSLGDARAMWAIREGIPEA
                     HARAGGNVKHDIALPVSAIPDFVAATDAQLSACFPWIQPSTFGHLGDGNLHYNMGTKD
                     GVPLAVAFEHQKEISRIVHDAVAARGGSISAEHGLGQLKRDDIVHYKPAVELELMRRI
                     KQALDPLHLMNPGKVI"
     gene            5577..6023
                     /locus_tag="AC16HB_09985"
     CDS             5577..6023
                     /locus_tag="AC16HB_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607159.1"
                     /translation="MRFFVFLAALALTGSAAAQTVRCESPDGKVTYAQNDCPRGTQPV
                     RTLPAPGKVPREDQRAAAERARNDAKALGRIESERKVEATRTAKAERTDRFRAEARAR
                     TCKRLALRLKQAKDDADAAPLNRAPDAKRKLKKAQENYELECSAAK"
     gene            complement(6024..6977)
                     /gene="dusA"
                     /locus_tag="AC16HB_09990"
     CDS             complement(6024..6977)
                     /gene="dusA"
                     /locus_tag="AC16HB_09990"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR5607160.1"
                     /translation="MLDWTDRHCRYFHRTLSRRARLYTEMVTTGALLHGDAARHLDFD
                     AAERPVALQLGGSEANELAQCARLGERWGYDEINLNCGCPSERVQRGSFGACLMAEPR
                     LVADCVKAMQDAVSIPVTVKHRTGLDRSEDDGLVRDFVGALFGAGCRVFIVHARNAWL
                     KGLSPKENRDVPPLRYATVHRLKHDFPDAVFVLNGGLHSLAEACAQLTHVDGVMLGRA
                     AYHDPYLLAQVDPTLFGADATIPARSDIVAAMSDYLACRVAAGESPRAIVSPMLGLYL
                     GQPGARHWRRMLSDPAFLERAGADALAAAAGALNAREPLEA"
     gene            7068..7721
                     /locus_tag="AC16HB_09995"
     CDS             7068..7721
                     /locus_tag="AC16HB_09995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607161.1"
                     /translation="MRYEERSTRAATHAAVQPVEGFSAPPAPESGDRAAFLLEFLRHP
                     RQIGSVVPSSAFLERRLVRAADAANARVVVELGPGTGGTTRALLRALRADAHLLAIDL
                     SPTFCARLAARARDPRLTVARGSAEHIDEFLRQWRLPRPDAVVSGIPFSTMPPAVADD
                     IARAIAAVLVPGGRFVAYQVRADVARYATPYLGAPVKRWEWRNVPPLQVFTWTKVKA"
     gene            complement(7711..7932)
                     /locus_tag="AC16HB_10000"
     CDS             complement(7711..7932)
                     /locus_tag="AC16HB_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HIR5607162.1"
                     /translation="MESTLFGLTEQQISEFGLTWGIGAFIALMLFIIWNVAKESKAGR
                     FGTFVLFFVLAFGIFGFLVKQLIQWLLRL"
     gene            complement(7938..8723)
                     /locus_tag="AC16HB_10005"
     CDS             complement(7938..8723)
                     /locus_tag="AC16HB_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HIR5607163.1"
                     /translation="MNSATAPSRRPLPVNRVGVSRPFAWLELGYRDLVAAPGASLLHG
                     LLIAVGGLAILTLTLRAWLLMPGALTGFVLVGPILATGLYELSRRRALGERPRLAHAV
                     AAWRRGTRPLVLLGVVLFCAGTAWVLVSALLFRYFVSARIVSPLDFLRYAAADQGAQF
                     FWLWVIAGGLGAAIVFALTVVSAPLLLDRSVDVRRALLTSARAVGDNPLPMALWAALI
                     MALSVLSMATMMIGFVFTVPIIGHATWHAYRDLVDAEAVPPRI"
     gene            8814..>9061
                     /locus_tag="AC16HB_10010"
     CDS             8814..>9061
                     /locus_tag="AC16HB_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607164.1"
                     /translation="MAVQNLARNMLEHTTQHTPLELARAAQRLRDETDLLCALEARGG
                     LSQRAPTASFDIEVWLIDRGGRPAPQSGSLVAAIQSPT"
BASE COUNT         1508 a         3130 c         3112 g         1311 t
ORIGIN      
        1 cggccggccg gcaggtaggc ggggtcgacg tcgtcgtcgt ccgtggagct cgtgatgcgg
       61 cggaaggtgc cgccggcgag cccgccgctg gtcatgtcgt attcccagat gttccagcgg
      121 ccggtgcatg caggctggtt gtcgatggtc gcggtgttcg cggtcgggca gcgcatcgcg
      181 aacacgatct tcttcccgtc gtaggacacc tcgggatcgg acacgtcgcc ctgcccctgc
      241 gtgaaacgct cggtgatgtt gtgctcaggc gcgctcggcg acgacttttc ccggatcatc
      301 agatcgccgc ccggtgcggt gggtgcaccg ttggttggat tggcccggat gctgttagcg
      361 cgcatcgcgt acgcgagcgc gacatcgccc gcgaccgtca cggtcgctcc atcgctggac
      421 gagcccttgc agccggcgag cagcgcgctc gcggccatga tcacgactcc aacactgccg
      481 acgcgttcaa atagtctcaa ggttgcctcc cgactcgtcc cggtcttctc actgccgagt
      541 tgggttgcct ccggccagca ggccggaaga gaagtgcaat tgacccagag cgtcaacggc
      601 caccgcgtcg acgccggaga tggattcgat cagttgcagc ccgcgcgcgg cgccgagaac
      661 gaacaccgtc ttcgacagtg cctcgctggt caggccgtcg gcggcaacga tggtcacgct
      721 gcgcacgccg cgcggcgact cgccggtgga cgggtcgatc aggtgatgat gacgcacacc
      781 gtcgagttcg aagcagcgtt cgtagtcgcc cgaggtcgag actgccgcct cctcgagcgg
      841 cagcagcgca atcacctcgc ctgcgctgcg cggatcgcgg atgccgatcg tccagggccg
      901 gccgcggcgg tcgccgacaa tgtggctgtc gccgccggca gtgacgatcg cgtgcgcgat
      961 gccacggcgg cgcaggatcg ccgcgccgtt gtcgaccgcg taacccttgg caaatccgcc
     1021 gaggtcgatg cgcatgcctg gacgtgcgaa gcggaccgtg cgcgcgcgcc ggtcgagcag
     1081 cagattccga tagcccaccg cggtgcgcgc gcgggcgatc tccgcctcgc tcggccggat
     1141 gccacggcgg tagtcgtaca aatggcccac cgccgcgtac gtgatgtcga aagcgccccc
     1201 ggacagtcgc gagaactcga tcgagcgcgc cagcagatcg aacaattcgt cgctgatctg
     1261 caccgggcct gcggccgcgt cgcgattgat gcgcgacagt tcagaatccg gcttgtacgg
     1321 actcatctgc gcgtcgatgc gatgcatctc ctgcattacg gcatcgatcg cgggctgcgc
     1381 cgatgcacga tcgtcgctcc acaactcgac acgaatggcc gtgcccatga tcgcctcgtc
     1441 acggctgaac cacccgtgct gcgagttctg gacgtcgcgt ttgcgcgcgg tccagggcat
     1501 caggcttgcg cttgcttgca ttcgcgtttc ttcggcgcac gaagcccttg cacgtgtggt
     1561 tcgaccactc gcgcatcaag gcgacctgcg tcctgagtgt tggcgttgac ggttacgaag
     1621 ccggtgcgcg ctttatagcg gcgagttgac gccgcgcaac gcgcgctcgt ccttcgtatt
     1681 ggggaatccg ttcaagcgca gcgcagtgct cactcgcaaa ccaatcgact gtggacgcgg
     1741 caacgcgcac tgggatgaca ctgaattgcg cgtgccgcct tcgaatcgac acgcggatcg
     1801 cttcggcgca acggatgaag cgcgcgcgag tccgcccgca taaggcgccg cgcgctcagt
     1861 tcgtgcgcag cacttcgtcg agcggacgtg caatccagtc cgcgtgcaat cccgcatcga
     1921 gccaggcgcc gagcccgtcg gcgtcgcgcg cggcgagcgt gttggcggga tcgactgcga
     1981 agaagcggaa ggcatcgacc agattcgcgc gcgccggatc acacgtgagc agccagctgc
     2041 ccgcgtgcgc gccggcgagc ggcgcgacgt agccgagccg ctccagcgcc atcagcgtgc
     2101 gctccgcgtc tgcgggcgcg ctgcgcacgg cgcgcgccag ggcctcgagc ggcaaacggc
     2161 cgtcgttgtc gccccgccgc cgcgcacgat gcagcgcgcg cagcagcgcg accgcagtga
     2221 tgaacgcgtt gccggcgcgt gattcgtctg cgaagcgcat ggcccgcagc atcggcaccg
     2281 tggcggcgat cgcggcgccg aagaggatga ccagccacga caggtagacc catatcagga
     2341 acaacggcag caccgcgaac gcgccgtaga tgttcgcgac ggtgccggtg cggatgtacc
     2401 acgcgaaacc gtcgcgcatc agctcgccca gcacggccgc gatcagcgcg ccgatgccgg
     2461 cgtcgcgcca atgcaccctg cggttcggca cgaccatgta tgcagcggcc accgcgctca
     2521 cgcccagcac gaacggcgtg tactcgagca cggtgagcag cgcacccggc acccgttcgc
     2581 tccatccggc cgatgcagtt gcgacgtagg acgccgcact gagactcgcc ccgatcacca
     2641 gcggtccgag cgtgagcagg ctccagtaca gcagcacgcg ctgcagcagc ggccggcgcg
     2701 tgcgcacctg ccagatgtcg ttgaagatgt ggtcgaccgt caggatcatc gacagcgcgg
     2761 tcagcagcag gaagaacagg ccgaatgccg tcagccgtcc cgccttcgcc gtgaactcgt
     2821 tcaggtagcg caggatcagc ggcgcgtact gctcgggcag caggccgcgg aacaggctct
     2881 tctccagcga cacgcggaac tcggtgaaca gtggaaacgc ggtgaacagc gacagcgcca
     2941 ctgccagcag cggcacgatc gacagcacgg tggtgaacgt caggccgccc gcgacctgcg
     3001 ccagccgcac ttcgtgtgcg cgtcgcaacg cgaagcgcag cacgtcacgc gtctgtgccg
     3061 tccaaggccc gaagcgttgc ccccactgat cgacgcgcag caacgccttg atcgatccga
     3121 cgagcgtcgc ctgcgcgcgt gcgaagagca tcgatgccat gcggtatggt agcggctcgt
     3181 cgttcctgcg aggccgagtc tggagatcct ggtcctttac tacagtcgtc acggcgccac
     3241 gcgcaagctg gccgagcacg tggccaccgg catcgatgcg gtcgccggcg cgcacgcgcg
     3301 gctgcgtacc gtaccgccgg tggctgcggt gacgcagacc gcagccaagc cggtgccgcc
     3361 cgacggcgcg ccgttcgccg aactgcgcga cctggaggag tgcgcagcgc tggccctcgg
     3421 ctcgccgacg cgcttcggca acatggccgc gccgctgaag cacttcctcg acggcaccag
     3481 cggtcactgg ctggcaggca cgctggccgg caagccggcc gcactcttca catcgacctc
     3541 ttcgctgcac ggcggccagg agagcacgct gctgtcgatg atgattccgc tcctgcatca
     3601 cggcatgctg atcgtcggcc tgccatacac cgaaccggag ctgatgacga cgacgacggg
     3661 cggtgggccg tatggcgcca gccatatcgc gcgccagcag ggagccgcgg cgatctccga
     3721 cgaggagcgt cgcctcgcgg tcgcgctcgg gcggcggctg gcgacgatca ccgtgaagct
     3781 gcgcgcatga cgaccgtggc gctgacgcga atgcaacgcg ccgccgggcg gatcgccgcc
     3841 gcaagctggc tcgcgctgct cgcgctcgcg gtgctgtggg aatggctgct ggcgccgctg
     3901 cgcccaggcg gctcttggtt gattctgaag gccgttccgc tgctcgttcc gctgcgcggg
     3961 gtctggcgcg gtgaaccgta tgctctccaa tgggcgctgc tgctgacacc cttttatttc
     4021 tgcgagggcg tcgtgcgcac gttcgatgcg gtgccggtcg cgcgactggc gtggatcgaa
     4081 gcgctcctcg cggtcgtctt cttcgtcgcc gcgatcgtct atctgcggcc gttcaagcgc
     4141 gccgcgcgca ggacgggacc gggatgaacc tgctcgagca actgacgacg atcgtcgggg
     4201 tcggaaacgt gctcatcggg tccgacgccg ggccctacct caccgactgg cgccggcgct
     4261 acacgggcgc ggcgctcgcg gtcgtgcgac cgggcacggc cgaagacgtg acgcgggtcg
     4321 tgcgcgcgtg catcggcatg cgcgcaccga tcgtgccaca gggcggcaac accggactgg
     4381 tcggcggcgg cacgccggac gcgacgggac gtgcagtcgt cgtctcgctg gcgcggatga
     4441 accgggtgcg cgcgctcgat acggcgaacg acgcgatcac cgtcgaggcg gggtgcgtgc
     4501 tgcagaacgt gcaggacgcg gcgcgcgcgg cgcaacggct gttcccgctg tcgctcgcgg
     4561 ccgaaggcag cgcaacgatc ggcggcaatc tttcgaccaa tgccggcggc acgcaggtgc
     4621 tgcggtatgg caatgcgcgc gacctgacgc tcggactgga ggtcgtgctg ccgtccggcg
     4681 aactctggga cggcctgcgc agtctgcgca aggacaatac cggctatgac ctgaagcacc
     4741 tgttcatcgg cgccgagggc acgctcggca tcatcacggc ggcgacgctg aagctgtatc
     4801 cgctgccggc ggcgcaggtc accgtgtttg cgagcgcgtc ctcgctgcac gcggcgctgg
     4861 cgctgctgaa ccgcgcgcgc gccgccgcag gcccggcgct gaccgcgttc gaagtgatgt
     4921 cggacctttg cctgcgtcgt ttcgcgcgcg agttccccga cgtgcggctg ccgctgccgc
     4981 tcgcgtcgcc gtggttcgcg ctgctggagc tgtcggacca tgagagcgcc gagcacgcgg
     5041 cggcgcgcct cgagtcgatc ctcggcgcgg cgctcgaggg tcgcgaagtc gacgacgcgg
     5101 tgatcgcaaa gtcgctcggc gatgcgcgtg cgatgtgggc gatccgcgag ggtatccccg
     5161 aagcgcacgc gcgtgccggc ggcaacgtca agcacgacat cgcgttaccg gtgtcggcga
     5221 ttccggactt cgtcgccgcg accgatgcac aactgtcggc atgctttccg tggatccagc
     5281 cttcgacgtt cggccacctt ggcgacggca atctgcacta caacatgggc accaaggacg
     5341 gcgttccgct cgcggtggcg ttcgagcacc agaaggagat cagccggatc gtccacgacg
     5401 cggtcgccgc gcgcggtggc tcgatcagtg ccgagcacgg gctcggacag ctgaaacgcg
     5461 acgacatcgt gcactacaag ccggccgtcg aactggagtt gatgcggcgg atcaagcaag
     5521 cgctcgatcc gctgcacctg atgaatcccg gcaaggtgat ctgaggagga agtgcgatgc
     5581 ggttctttgt tttcctggcg gcactcgcgc tgacgggcag tgcagcggcg cagacggtgc
     5641 gctgcgagtc gcccgacggc aaggtcacct atgcgcagaa tgactgcccg cggggcacgc
     5701 agccggtgcg caccctgccc gcgcccggca aggtcccgcg cgaggaccag cgcgcggcag
     5761 ccgaacgcgc gcgcaacgat gccaaggcgc tcggccgcat cgaaagcgag cgcaaggtcg
     5821 aggcgacacg cacagccaag gccgaacgaa ccgaccgctt ccgtgccgag gcacgcgcac
     5881 gcacgtgcaa gcgcctcgcg ttgcgcttga agcaggcgaa ggacgacgcc gatgcggctc
     5941 cgctgaaccg cgcgcccgac gccaagcgca agttgaagaa ggcgcaggag aactacgagc
     6001 tggagtgcag cgccgccaag tgatcaggcc tcgagcggct cgcgcgcgtt caacgcgccg
     6061 gccgcggctg cgagcgcatc agcccccgcg cgctcgagaa acgccgggtc gctgagcatg
     6121 cggcgccagt ggcgcgcgcc cggctggccc aggtacagcc cgagcatcgg cgacacgatc
     6181 gcgcgcggac tctcccccgc ggcgacccgg catgcaagat agtcggacat cgcggccacg
     6241 atgtcagaac gcgccggaat cgtcgcgtcg gcgccgaaca gagtcgggtc gacctgcgcc
     6301 agcagatatg gatcgtgata cgcggcgcgg ccgagcatca cgccgtcgac gtgggtaagc
     6361 tgcgcacatg cctcggccag cgagtgcagc ccgccgttca gcacgaacac agcatcggga
     6421 aagtcgtgct tcagccggtg cacggtcgcg tagcgcaggg gcggcacgtc gcggttttcc
     6481 ttcgggctga gccccttgag ccacgcgttg cgcgcgtgca cgatgaacac gcggcagccg
     6541 gcgccgaaca gcgcgccgac gaagtcgcgc accagcccat cgtcctcgct gcgatcgagc
     6601 ccggtccggt gcttgaccgt gaccgggatc gacaccgcgt cctgcatcgc ctttacgcag
     6661 tcggccacca gccgcggttc ggccatcagg caggctccga acgagccgcg ctgcacgcgc
     6721 tccgaggggc agccgcagtt cagattgatc tcgtcgtagc cccagcgttc gccgaggcgc
     6781 gcgcactgcg ccaactcgtt ggcctcggag cccccgagct gcaatgcaac cggccgctcg
     6841 gcagcatcga agtccagatg gcgcgccgcg tcgccgtgca gcagcgcgcc ggtcgtgacc
     6901 atctccgtgt acagtcgcgc gcggcgcgac agcgtgcgat ggaagtaccg gcaatgacgg
     6961 tcggtccagt cgagcatcgg cgccacgcaa aagcgccacg gcgacgcagg cagcgaagca
     7021 accgttggta aagtcacggc ggaggaggct gagaactgga gaggattatg cgttacgaag
     7081 aacgttcgac ccgcgcggcg acgcacgcgg ccgtgcaacc cgtggaaggt ttcagcgcgc
     7141 cgccagcccc ggaatcgggg gatcgcgccg catttctgct ggagttcctg cgccatccgc
     7201 gccagatcgg ctcggtggtg ccgagctcgg catttctcga gcggcggctg gtgcgcgcgg
     7261 ccgacgctgc caacgcgcgc gtcgttgtcg aactcggccc ggggacggga ggcaccacgc
     7321 gcgcgctgct gcgcgcgctg cgcgcggacg cgcacctgct cgcgatcgac ctgtcgccga
     7381 ccttctgcgc gcggctggcg gcccgcgcgc gcgatccacg gctgacggtt gcgcgcggca
     7441 gcgccgagca catcgacgaa ttcctgcggc aatggcggct gccacgtccc gatgccgtcg
     7501 tctccgggat accgttctcg accatgccgc ccgcggtggc cgacgacatt gcgcgcgcga
     7561 tcgccgctgt gctggtgccg ggcggacgct tcgtcgccta ccaggtgcgc gccgacgtcg
     7621 cgcgctacgc gacgccttac ctcggcgcgc ccgtcaagcg ctgggaatgg cggaacgtgc
     7681 cgccgctgca ggtgttcacc tggacgaagg tcaaagcctg agcagccact ggatcaactg
     7741 cttcaccagg aagccgaaga tcccgaatgc gagcacgaag aacagcacga acgtgccgaa
     7801 gcgaccggcc ttcgattcct tcgccacgtt ccagatgatg aacagcatca gcgcgatgaa
     7861 cgcgccgatg ccccaggtca gtccgaattc ggagatctgc tgctcggtca gcccgaagag
     7921 cgtcgactcc atcgcggcta gatcctcggc gggaccgcct ccgcatcgac cagatcgcga
     7981 taggcgtgcc acgtcgcgtg cccgatgatc ggcacggtga atacgaagcc gatcatcatc
     8041 gtcgccatcg acagcaccga cagcgccatg atcagcgcgg cccacagcgc catcggcaac
     8101 ggattgtcac cgaccgcgcg cgcgctggtc agcaacgcgc gccgcacgtc gaccgaccga
     8161 tcgagcagca gcggcgcgga cacgacggtc agcgcgaaca cgatggccgc gcccagcccg
     8221 cccgcgatca cccacagcca gaagaactgc gcgccctggt ccgcggccgc atagcgcagg
     8281 aagtcgagcg gcgagacgat ccgcgcgctg acgaagtaac gaaacagcag ggccgagacc
     8341 agcacccagg ccgtgccggc gcagaacagc acgaccccga gcaggaccag cggccgcgta
     8401 ccgcgccgcc acgctgccac cgcgtgtgcg agccgcggcc gctcgccgag cgcacggcgc
     8461 cggctcagtt cgtacaagcc ggtggcgagg atcggcccga ccagcacgaa gccggtcagc
     8521 gcacccggca tcagcagcca cgcgcgcagc gtcagcgtca ggatcgcgag cccgcccacg
     8581 gcgatgagca gcccgtgcag caggcttgca cccggcgcgg caacgagatc gcgatagccg
     8641 agttcgagcc acgcgaacgg gcgcgacacg ccgacgcgat tgaccggcaa tggccggcgc
     8701 gaaggtgcgg tcgccgaatt cataccgggg gcagcttaat cgacgtccga agagactact
     8761 gcgcgcggcg gacctgacgc gcggcgtagc gtgtgcgagc atcgctgcct gcaatggccg
     8821 tgcagaacct cgctcgaaac atgctcgagc acaccacgca gcacactccg ctggaactgg
     8881 cacgcgccgc gcagaggctg cgcgacgaga cggatctgct gtgtgcgctc gaagcgcgcg
     8941 gcgggctttc gcagcgcgca ccgacggcga gcttcgacat cgaggtgtgg ctgatcgatc
     9001 gcggcggccg accggcacca cagtccggct cgctggttgc cgcaattcag tcgccgacga
     9061 t
//
LOCUS       DBNHBY010000401         4349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1173703, whole genome shotgun sequence.
ACCESSION   DBNHBY010000401 DBNHBY010000000
VERSION     DBNHBY010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1173703"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="AC16HB_10015"
     CDS             <1..1254
                     /locus_tag="AC16HB_10015"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I 51 kDa subunit family protein"
                     /protein_id="HIR5607165.1"
                     /translation="AASNQRSWREGFDALAQGPVETLDEIKRSKLRGRGGAGFTVGMK
                     WEACRNARAAQRYVVCNADEGEPGTFKDRALLTRHADLVIEGMTVAAYVVGATKGFLY
                     LRGEYRHLLAPLNEVLARRRAQGLLGSGICGRPDIEFDLEIHLGAGAYVCGEESALIE
                     SLEGKRGVPRNRPPYPVTSGFLKFPTVVNNVETFCAAALIARHGGDWYARIGTPRSTG
                     TKILSVSGDCARPGIYEYPFGVTVQQVLDDCGAEMTQAVQVSGPSGICIDATEFGRRL
                     AFEDLPTAGAMMVFDESRDMFEVARNFAHFFAHESCGFCTPCRVGTPLLRNLMDKLAA
                     GKGSQHDLNRIGELNHLLQAASHCGLGHTACNPILDTLKRFRPAYEQRLVSMEYEPAF
                     DLDGALARARAMTGRDDAGAHLAEF"
     gene            1254..2000
                     /locus_tag="AC16HB_10020"
     CDS             1254..2000
                     /locus_tag="AC16HB_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5607166.1"
                     /translation="MNEDRPTFLLDGNEVPFSTGQTIVQAALAAGRYVPHLCYHPEFK
                     PHGSCKLCTVKADGRWITSCTTAAQAGMQVESNTDEINAERRLLVQMLFVEGNHFCPS
                     CEKSGNCRLQAVAYDLGMVSTQLDHFYPDRPLDASHPDILLEFNRCILCELCVRASSE
                     VDGKGVFALSGRGITKHLIVNSESGRLVDTNIAVSDKAIDVCPVGVILRKRVGFAQPI
                     GQRRFDARPISAQALRDAPRPLDRKPSGAE"
     gene            1997..2539
                     /locus_tag="AC16HB_10025"
     CDS             1997..2539
                     /locus_tag="AC16HB_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5607167.1"
                     /translation="MMARRFRVATTSLAGCFGCHMSFLDIDERLFPLIERIEFDRSPL
                     TDVKRCGKCDIGFIEGGICNAENVHVLREFRKNCRILVAIGACAINGGLPAQRNHLDL
                     GQCLQEVYVTEPGLAKGGIPNDPELPLPLDKVHPLHEVVHIDYFLPGCPPSADAIWKF
                     LNDLLSGRTPQLGHALMHYD"
     gene            2539..4014
                     /locus_tag="AC16HB_10030"
     CDS             2539..4014
                     /locus_tag="AC16HB_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5607168.1"
                     /translation="MSFELETAANPESLRRVAIDPVSRVEGHGKVTILLDADGHVHQV
                     RLHIVEFRGFEKFIQGRPYWEVPVMVQRLCGICPVSHHLAASKALDMVLGAKLTPTAE
                     KVRRLMHYGQILQSHALHFFHLSAPDLLFGFDSEVAKRNIVGVAAAHPEIAKQGVLLR
                     KYGQEVIRVTAGKRIHGTGSVPGGVNKALTLAERDALLADIYQVIAWSRDAVELARKL
                     HLANVSLYNSFGAFRSNMLSLVAADGALDLYDGALRARDETGAILFDHFDTRRYWAVI
                     VEEVRPWSYMKFPYLRAFGPERGWYKVGPLARVQNCDHMATPFADVARREFIDFGGSQ
                     PVHATLAYHWARMIEMLFAAECIKELLHDEDLTGTELMAPRGERVERGVGVIEAPRGT
                     LIHHYRVNEEDLVVRANLIVSTTHNNQAMNEAIRQVARRYLDGRKLTEGLLNHIEVAI
                     RAFDPCLSCATHALGRMPLAVELVDVDVSLADRLVKGTAAC"
     gene            4008..>4349
                     /locus_tag="AC16HB_10035"
     CDS             4008..>4349
                     /locus_tag="AC16HB_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5607169.1"
                     /translation="MLTLDAPAGVKPLLVIAVGNPSRGDDAIGPLLAERLAARELPEV
                     EVLVDYQLQVEHALDLIGRRRVVFVDASASGAAPYYLNEARADADDSVTSHALSPAAV
                     LNCHRRVTGAAP"
BASE COUNT          737 a         1448 c         1461 g          703 t
ORIGIN      
        1 gccgcatcga accagcgctc gtggcgcgaa ggattcgatg ccttggcgca aggtccggtc
       61 gaaaccctcg acgagatcaa acgatccaag ctgcgtggcc gcggcggtgc gggcttcacc
      121 gtcgggatga aatgggaagc atgccgcaac gcgcgcgccg cgcagcgcta cgtcgtctgc
      181 aacgccgacg agggcgagcc gggcacgttc aaggaccgcg cgctgctgac gcgccacgcc
      241 gacctcgtga tcgagggcat gacggtcgcg gcgtacgtgg tcggcgcgac caagggcttt
      301 ctctacctgc gcggcgagta tcggcatctg cttgcgccgc tgaacgaggt tctcgcgcgg
      361 cgccgtgcgc agggactgct cggcagcggc atctgcggca ggcccgacat cgagttcgac
      421 ctcgagatcc atctcggcgc gggagcgtat gtgtgcggcg aggagtcggc gctgatcgaa
      481 tcgctcgagg gcaagcgcgg ggtcccgcgc aaccggccgc cgtatccggt gacgagcggg
      541 tttctgaaat ttccaacggt cgtgaacaat gtcgaaacct tctgcgcagc cgccctcatc
      601 gcgcggcacg gcggcgattg gtacgcgcgc atcggcacgc cgcgctcgac cgggaccaag
      661 atcctgtcgg tctcgggcga ctgcgcgcgg ccgggaatct acgagtaccc gttcggcgtt
      721 accgtgcagc aggtgctcga cgattgcggc gccgagatga cgcaggcggt gcaggtcagc
      781 gggccatcgg gaatctgcat cgacgccacc gaattcgggc gccggctcgc gttcgaggac
      841 ctgccgaccg cgggcgcgat gatggtgttc gacgaatcgc gtgacatgtt cgaggtggcg
      901 cgcaacttcg cgcacttctt cgcccacgag agctgcggct tctgcacgcc ttgccgcgtc
      961 ggcacgccgc tgctgcgcaa tctgatggac aagctcgcag ccggcaaggg ctcccagcac
     1021 gacctgaacc ggatcggcga actgaaccac ctgctgcagg cggcgagcca ctgcggactc
     1081 ggacataccg cgtgcaaccc gatcctcgac accctcaagc gcttccgacc cgcgtacgag
     1141 cagcggctgg tatcgatgga atacgagccg gcgttcgacc tggacggcgc attggcgcgc
     1201 gcgcgcgcga tgaccggccg cgacgacgcc ggcgcacacc tggcggaatt ctgatgaacg
     1261 aagaccggcc gaccttcctg ctcgatggca acgaggtgcc gttttcgacc gggcagacga
     1321 tcgtgcaggc cgcgctggcc gccgggcgtt acgtgccgca cctgtgctac cacccggagt
     1381 tcaagccgca cggcagctgc aaactttgca cggtcaaggc cgacggtcgc tggatcacct
     1441 catgcaccac cgcggcgcag gccggcatgc aagtcgagag caacaccgac gagatcaacg
     1501 ccgagcgccg gctgctggta cagatgctgt tcgtcgaggg caaccacttc tgcccttcgt
     1561 gcgagaagag cggcaactgc cggctgcagg cggtcgctta cgacctcggc atggtgagca
     1621 cgcagctcga ccacttctat cccgaccggc cgctcgacgc gtcgcacccc gacatcctgc
     1681 tcgagttcaa ccgttgcatc ctgtgcgaac tgtgcgtgcg tgcgtcgagc gaagtcgacg
     1741 gcaagggtgt gttcgcgctg tcggggcgcg gcatcaccaa gcacctgatc gtcaacagcg
     1801 agtcgggacg gctggtcgac acgaacatcg cagtctccga caaggcgatc gacgtctgcc
     1861 cggtcggcgt gattttgcgc aagcgcgtcg gctttgctca gccgatcggc cagcgtcggt
     1921 tcgatgcgcg gccgatcagc gcgcaggcgc tgcgcgacgc gccgcggccg ctcgaccgca
     1981 aaccgagcgg ggccgaatga tggcgcgccg tttccgggtc gcgaccacgt cgctcgccgg
     2041 ctgcttcggc tgtcacatgt cgttcctcga catcgacgag cgcctgttcc cgctgatcga
     2101 gcgcatcgag ttcgaccgct cgccgctcac cgacgtgaag cgctgcggca agtgcgacat
     2161 cggcttcatc gaaggcggga tctgcaacgc ggagaacgtg cacgtgctgc gcgaattccg
     2221 gaagaactgt cgcatcctgg tcgcgatcgg cgcctgcgcc atcaacggcg ggctgccggc
     2281 gcagcgcaat cacctcgacc tcggccagtg cctgcaggag gtctatgtga cggagccagg
     2341 gctggcgaaa ggcggcatcc cgaacgaccc ggagctgccg ctgccgctcg acaaggtgca
     2401 cccgctgcac gaggtggtgc atatcgacta cttcctgccg ggttgtccgc cgtccgccga
     2461 tgcaatctgg aagttcctca acgacctgct gtccggccgc acgccgcagc tcggccacgc
     2521 attgatgcac tacgactgat gagcttcgaa ctcgaaaccg ctgccaatcc ggaaagcctg
     2581 aggcgcgtcg cgatcgatcc ggtgtcgcgc gtcgaagggc atggcaaggt cacgatcctg
     2641 ctcgatgccg acgggcacgt gcaccaggtg cggctgcaca tcgtcgagtt ccgcggcttc
     2701 gagaaattca tccagggccg gccatactgg gaagttccgg tgatggtgca gcggctgtgc
     2761 ggcatctgcc cggtcagcca tcacctcgcg gcgtcgaagg cactcgacat ggtcctcggt
     2821 gcgaagctca cgcccaccgc cgagaaggtg cggcggctga tgcactacgg ccagatcctg
     2881 cagtcgcacg cgctgcactt cttccacctg tcggcgcccg acctgctgtt cggcttcgat
     2941 tcggaggttg ccaagcgcaa catcgtcgga gtcgccgcgg cgcatccgga gatcgcgaag
     3001 cagggcgtgc tgctgcgcaa gtacggccag gaggtgatcc gcgtcaccgc cggtaaacgc
     3061 atccacggca cgggcagcgt cccgggcggc gtgaacaagg cgctgacgct tgcggagcgc
     3121 gatgcgctgc tcgccgacat ctatcaggtg atcgcatggt cgcgtgacgc ggtcgaactc
     3181 gcgcgcaagc ttcatcttgc gaacgtgagc ctgtacaaca gcttcggcgc gtttcgctcc
     3241 aacatgctga gcctggtcgc ggccgacggc gcgctcgatc tgtacgacgg cgcgctgcgc
     3301 gcgcgcgacg agaccggcgc gatcctgttc gaccacttcg acacccgccg ctactgggct
     3361 gtaatcgtcg aggaagtgcg gccctggtcg tacatgaagt ttccgtacct gcgggcgttc
     3421 gggcccgagc gcggctggta caaggtcgga ccgctggcac gggtgcagaa ctgcgaccac
     3481 atggccacgc cgttcgcgga cgttgcgcgg cgcgaattca tcgacttcgg cggcagccag
     3541 ccggtgcacg ccacgctcgc ctaccactgg gcgaggatga tcgagatgct gttcgccgcc
     3601 gagtgcatca aggaactgct gcacgacgag gatctcacgg gcaccgagct gatggcgccc
     3661 cgcggcgagc gcgtcgagcg cggcgtcggc gtgatcgagg cgccgcgcgg cacgctgatc
     3721 catcactatc gcgtgaacga ggaggacctc gtcgtacgcg cgaacctgat cgtttccacg
     3781 acgcacaaca accaggcgat gaacgaagcg atccgtcagg tcgcgcggcg ctatctcgac
     3841 ggccgcaagc tgaccgaggg tctgctcaat cacattgaag tcgcgatccg cgcgttcgat
     3901 ccctgcctgt cgtgcgcaac acacgcgctc ggcaggatgc cgctcgcggt cgaactggtc
     3961 gacgtcgatg tttcactcgc cgatcggctc gtgaagggta cggccgcatg ctgacgctcg
     4021 acgcgccggc gggcgtgaag ccgctgctcg tgatcgcggt cggcaatccc agccgcggcg
     4081 acgacgcgat cggaccgttg ctggcggagc ggctcgccgc gcgtgaactg ccggaggtcg
     4141 aggtactggt tgattaccag ttgcaggtcg agcacgcgct cgacctgatc ggccgcaggc
     4201 gggtcgtgtt cgtcgatgca agcgcaagcg gcgcggcacc gtactacctc aatgaggcac
     4261 gtgcggacgc ggacgacagc gtcacgtcgc acgcgctgtc gccggctgcc gtcctgaact
     4321 gccatcggcg cgtaaccggt gccgcgccg
//
LOCUS       DBNHBY010000402         6068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_903420, whole genome shotgun sequence.
ACCESSION   DBNHBY010000402 DBNHBY010000000
VERSION     DBNHBY010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_903420"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="AC16HB_10040"
     CDS             complement(<1..733)
                     /locus_tag="AC16HB_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HIR5607170.1"
                     /translation="MTAKFNILTLNNIAHSGLARLPHSLYAVGKHVDPPDAILVRSHD
                     MHAMDIAPSVKAIGRAGAGTNNIPVAALSKRGVPVFNSPGANANAVRELVLAALLIGA
                     RNLVPALQFVAALPSDAKDFDARVEDGKKQFAGVELPHRTLGIVGLGAIGGLVAETAL
                     KLGMKVIGFDPEITVDAAWRLPSGVRKANSIEELLKHSDFVTLHLPLAPATRGMINEQ
                     RLGVMKQGAVLLNFARDAIVDEAAIV"
     gene            complement(747..1826)
                     /gene="serC"
                     /locus_tag="AC16HB_10045"
     CDS             complement(747..1826)
                     /gene="serC"
                     /locus_tag="AC16HB_10045"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR5607171.1"
                     /translation="MQVFNFSAGPAVLPKEVLQRAADEMLDWHGSGMSVMEMSHRGKE
                     FISIAKKAEADLRTLLAIPDDYAVLFLQGGAIGENAIVPMNLLNGFKAIDFVNTGEWS
                     KKSIKEAKKYAQVNIAASAEDRNFTYVPARESWKLGKDAAYVHICSNETIGGVEYHWT
                     PDVGSVPLVADMSSHILSRVIDVRKYGVIYGGAQKNVGPAGLTLVIVRKDLLDRALPI
                     TPSAFHWKEQAESESMLNTPPTYAIYIAGLVFEWLLAQGGVAAIEQKNIAKAKLLYDY
                     LDASPFYVNPIAKADRSRMNVPFKLRDEKLDGEFLKGADARGLLQLKGHRSVGGMRAS
                     IYNAMPIAGVQALVDYMREFEKKNG"
     gene            complement(1902..2543)
                     /locus_tag="AC16HB_10050"
     CDS             complement(1902..2543)
                     /locus_tag="AC16HB_10050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5607172.1"
                     /translation="MTAYRLFGRPGWGSAIVEAQLAALGLPFDYEEVPDLFASEEGRR
                     SLARHNPLSQVPTLRLPDGSMMTESAAITLYLADATGRDDFVPPVANTARPQFLRWLV
                     FLVANVYPTFTYADDPQRFVRDEPAAKEFGATVDDYRGRLWRMVEAAAGAPWFLGERF
                     SALDIYIATMTHWRPGRRWFEENTPRLAAIARGAGEDARFLAVWRHNFPRDFG"
     gene            complement(2622..3599)
                     /locus_tag="AC16HB_10055"
     CDS             complement(2622..3599)
                     /locus_tag="AC16HB_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5607173.1"
                     /translation="MVELAEADRVEVLVIVDNVSDNLSSVPDYVETEMPRLWKRGLKR
                     WSGWCMCCAAHGLSCAITAWRGDAAHTLLFDTGPDNAVFERNVDRLGFDLGCVEGIVL
                     SHGHWDHAGAMLRALEMIHLRNGGRPIPTYMHPGMYRPRAMKGPDGSMRPFADVPSVE
                     QLEKHGAAVVNATAPLLILDDLFYVSGEIPRVTAFETGLQGQFRRTEDGADWEPDPLL
                     MDERFVAVGVKGKGVIVLTACSHAGVVNVMTHARDCFPGRKLHGVIGGFHLAGSNERV
                     IPETVAALRAFDLRSIAAAHCTGWRAVNALAAAFGDAVVPSAVGKLYQF"
     gene            3710..4489
                     /locus_tag="AC16HB_10060"
     CDS             3710..4489
                     /locus_tag="AC16HB_10060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607174.1"
                     /translation="MDREQIRSAVLALIESIAPDTDVRSLRPDQPLRRQIELDSMDWL
                     NVIVGLHERLAIDVPESDYGRLKTLDSIVDYLASRPARTSRAAAVTELPHARYVINDV
                     PVEVRPIRPDDMPLEADFVQHLSPDARYQRFMVTVNRLPERKLHYLTEVDQIRHVALV
                     ATVERDGRPVLVGVVRYIIDPGGNSCEFAIAIDDAWQRSGLAGILMLALLGVARARGL
                     ATMEGFVLTTNTRMLKFTRQLGFTQQRDPEDRDTVRVVRRL"
     gene            complement(4504..5643)
                     /locus_tag="AC16HB_10065"
     CDS             complement(4504..5643)
                     /locus_tag="AC16HB_10065"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR5607175.1"
                     /translation="MATRLVFLVPGFFGFTSVGSVSYFERVEQVLDRALREHGAAAQI
                     VRCMTQPTASIPRRTDALRRQVIRSGGLAARELHFVGHSTGGLDVRMLLTPGVQIGGD
                     GTAERIGRRTRTAIGVATPHHGTPLGNHFATIQGQTLLLVASALATSGQGRGAILAVA
                     KAIALVARLDDWLGRTEGPLDRIAEGLLRRIRFNRRDPVWRYLGEIERDQGAVLQLTP
                     EGIDLFNAAVIDRPGIDYGSIVTAVPKPSERLRNKDLLDPEYVALRAVFRLLHALAAR
                     PHPRYPYPQPSSAARRALERALGFSVTPVTNDGIVPTLSQLHGRVLHVARADHLDVVG
                     HYTLAGGRTADWLPSGAGFTSEAFEATWQAVAAAIARSSRGARAD"
     gene            complement(5757..>6068)
                     /locus_tag="AC16HB_10070"
     CDS             complement(5757..>6068)
                     /locus_tag="AC16HB_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5607176.1"
                     /translation="LLASRRECEEETGISVDGEFVALAPVRQPSGKVIHAFALRADVD
                     PAQLTSNTFALEWPPRSGQRREFPEVDRAAWFTLDEAAAKIQKGQAPFLDELRRLASD
                     A"
BASE COUNT          952 a         2136 c         2031 g          949 t
ORIGIN      
        1 cgacgatcgc agcctcgtcg acgatcgcgt cgcgcgcgaa attcagcagc acggcgccct
       61 gcttcatcac cccgaggcgc tgctcgttga tcatgccgcg cgtcgcgggt gccagcggca
      121 ggtgcagcgt gacgaagtcg gagtgcttca gcagttcctc gatgctgttg gccttgcgca
      181 cgccggacgg cagccgccac gcggcgtcga ccgtgatctc gggatcgaag ccgatcacct
      241 tcatcccgag cttgagcgcc gtttcggcca ccagcccgcc gatcgcgccc agcccgacga
      301 tgccgagcgt gcggtgcggc agttcgacgc cggcgaactg cttcttgccg tcctcgacgc
      361 gcgcgtcgaa gtccttggcg tccgacggca gcgcggcgac gaactgcagc gccggcacca
      421 ggttgcgcgc gccgatcagc agcgccgcca gcaccagttc gcgcaccgcg ttggcgttgg
      481 caccaggcga gttgaacacc ggcacgccgc gcttggacag cgccgccacc gggatgttgt
      541 tggtgcccgc gcccgcgcgt ccgatcgcct tgacgctcgg cgcgatatcc atcgcgtgca
      601 tgtcgtgcga gcgcaccagg atggcgtcgg gcgggtcgac atgcttgccg accgcgtaca
      661 gggaatgcgg caagcgcgcc agacccgagt gcgcgatgtt gttcagcgtc aggatgttga
      721 atttggcggt catggcaagg gcggggtcag ccgttcttct tctcgaactc gcgcatgtag
      781 tcgaccagcg cctgcacgcc tgcgatcggc atcgcgttgt agatcgacgc gcgcatgccg
      841 ccgaccgagc ggtgaccctt gagctgcagc aggccgcgcg cgtcggcacc cttcaggaac
      901 tcgccgtcga gtttctcgtc gcgcagcttg aacggcacgt tcatgcgcga gcggtccgcc
      961 ttcgcgatcg ggttgacata gaacgggctc gcatcgaggt agtcgtacag cagcttcgcc
     1021 ttggcgatgt tcttctgctc gatcgccgcc accccgccct gcgccagcag ccactcgaat
     1081 accagcccgg cgatgtagat cgcgtaggtg ggcggggtgt tcagcatcga ttcgctctcg
     1141 gcctgctcct tccagtggaa cgcgctcggc gtgatcggca gcgcgcgatc gagcagatcc
     1201 ttgcgcacga tcaccagcgt cagaccggcc gggccgacgt tcttctgcgc accgccgtag
     1261 atcacgccgt acttcctcac atcgatcacg cgcgacagga tgtgcgacga catgtccgcc
     1321 accagcggca cgctgccgac atccggcgtc cagtgatact cgacgccgcc gatcgtctcg
     1381 ttggagcaga tgtgcacgta cgcggcgtcc ttgccgagct tccaggactc gcgcgccggc
     1441 acgtaggtga agttcctgtc ctcggcggat gccgcgatgt tgacctgcgc gtacttcttc
     1501 gcttccttga tcgacttctt cgaccactcg ccggtgttga cgaagtcgat cgccttgaag
     1561 ccgttcagca ggttcatcgg gacgatcgcg ttctcgccga tcgcgccgcc ctgcaggaac
     1621 agcaccgcgt aatcgtcggg gatggcgagc agcgttcgca ggtcggcctc ggccttcttc
     1681 gcgatcgaga tgaactcctt gccccgatgg ctcatctcca tcaccgacat gccgctgccg
     1741 tgccagtcga gcatctcgtc ggcggcgcgc tgcaatacct ccttgggcag caccgcgggg
     1801 ccggcgctga aattgaatac ctgcatgggg atttccgggt tgatgggagg ggactgcgtc
     1861 gactgcgact ttaccggaat cccgcgggca cccgcgcgcc gctatccgaa gtcgcgcggg
     1921 aagttgtgcc tccacaccgc gaggaaacgc gcatcctcgc ccgcgccgcg cgcgatcgcg
     1981 gcgagccgtg gcgtgttctc ctcgaaccag cgccggccgg gacgccagtg cgtcatcgtc
     2041 gcgatgtaga tgtcgagcgc ggaaaagcgc tcgccgagga accacggcgc gcccgccgcc
     2101 gcctcgacca tgcgccacag ccggccgcgg tagtcgtcga cggtggcgcc gaattccttg
     2161 gcggcgggct cgtcgcgcac gaagcgctgc ggatcgtcgg cgtaagtgaa ggtcggatag
     2221 acgttggcga ccaggaacac cagccagcgc aggaactgtg gccgtgccgt atttgcgacc
     2281 gggggcacga agtcgtcgcg cccggtcgca tccgccagat agagcgtgat cgccgcgctc
     2341 tcggtcatca tcgacccgtc cggcagccgc agtgtcggca cctgcgacaa cgggttgtgg
     2401 cgcgccaggc tgcggcggcc ctcctccgac gcgaacaggt cgggcacctc ctcgtagtcg
     2461 aatggcaagc cgagcgcggc aagctgcgcc tcgacgatcg ccgagcccca gccggggcga
     2521 ccgaacaggc ggtaggcagt catggcggtc ctcgctttca cgaatcggca cgcatgatat
     2581 tgaaggcggt cgcgtgcctc ggccgcgcat gccgcgccag cctagaactg gtacagcttg
     2641 cccaccgcgg acggcaccac cgcgtcgcca aacgcggccg ccagcgcgtt caccgcgcgc
     2701 cagccggtgc aatgcgcggc cgcgatggac cgcaggtcga acgcgcgcag ggcggcgacg
     2761 gtttcgggga tgacgcgctc gttgctgccc gcgagatgga acccgccgat cacgccatgc
     2821 agcttgcgtc ccggaaagca gtcgcgcgcg tgcgtcatca cgttgacgac gccggcgtgc
     2881 gagcacgcgg tcagcacgat gacgcccttg cccttcacac cgacagcgac gaagcgctcg
     2941 tccatcagca acgggtcggg ctcccagtcc gcgccgtcct cggtgcggcg aaactgtccc
     3001 tgcaggccgg tttcgaacgc cgttacgcgc gggatctcgc cactgacata gaagagatcg
     3061 tcgaggatca gcagcggcgc ggtggcgttg accacggcgg cgccatgctt ctcgagttgc
     3121 tcgacgctcg gcacgtcggc gaacggacgc atcgacccgt ccggcccctt catcgcccgg
     3181 ggccggtaca tgcccggatg catgtaggtc gggatcggtc gcccgccgtt gcgcagatga
     3241 atcatctcga gcgcgcgcag catcgcgccg gcatggtccc aatggccgtg cgacagcacg
     3301 atgccttcga cgcagccgag gtcgaagccg agtcggtcga cattgcgctc gaacacggcg
     3361 ttgtccggcc cggtgtcgaa cagcagcgtg tgcgcggcgt caccgcgcca ggcggtgatc
     3421 gcgcacgaca gcccgtgcgc cgcgcagcac atgcaccagc ccgaccaccg tttcagcccg
     3481 cgcttccaca gccgcggcat ctcggtctcg acatagtcgg gaaccgacga caggttgtcg
     3541 gagacgttgt cgacgatgac caggacttcg acccggtccg cctcggcgag ctcgaccatg
     3601 gcaggcctcc cgttcggtca aggaacgcgg cacgacgcgc ggcagatcgt acgctcgccc
     3661 gctgacgtgg aagctagact ccggtgccat tcacagacga tcggaccccg tggaccgcga
     3721 gcagatccgc agcgccgttc ttgccctgat cgaatcgatt gcgccggata ccgacgtgcg
     3781 cagtctccgg cccgatcagc cattgcgccg gcagatcgaa ctggattcga tggactggct
     3841 caacgtgatc gtcggactgc acgagcggct ggcaatcgac gttccggagt cggactacgg
     3901 ccgcctgaag accctggact cgatcgtcga ctatcttgcg tcgcggcccg ccaggacaag
     3961 cagggcggcc gccgtgaccg agcttccgca tgcgcgctac gtgatcaacg atgtcccggt
     4021 cgaagtgcgg ccgatccgcc cggacgacat gccgctggag gcggacttcg tgcagcacct
     4081 atcgcccgat gcgcgctacc agcgcttcat ggtcacggtg aaccggttgc cggagcggaa
     4141 gctgcactac ctgaccgagg tcgaccagat ccgccacgtg gcgctggtgg ccacggtgga
     4201 gcgcgacgga cggccagtgc tcgtcggcgt ggtccgctac atcatcgacc ccggcgggaa
     4261 cagctgcgag ttcgcgatcg cgatcgacga tgcgtggcag cgctcggggc tggccggcat
     4321 cctgatgctc gcgctgctcg gcgtagcacg cgcgcgcggg ctggcgacga tggaaggatt
     4381 cgtgctgacg accaatacgc ggatgctcaa gttcacgcgc cagctcggct tcacccagca
     4441 gcgcgacccg gaggaccgcg acaccgtgcg cgtcgtgcgc aggctgtaac cagccggcgc
     4501 gcgtcagtcg gcgcgcgccc cccggctgct gcgtgcgatc gccgccgcaa ccgcctgcca
     4561 ggtcgcttcg aacgcctcgg acgtgaagcc cgcgcccgac ggcaaccagt ccgcggtgcg
     4621 cccgccggcc agcgtgtagt ggccgaccac gtcgaggtga tcggcgcgcg cgacgtgcag
     4681 cacgcggccg tgcagttgcg acagcgtcgg cacgatgccg tcgttggtga ccggcgtgac
     4741 gctgaacccg agcgcacgtt cgagggcacg ccgcgccgcc gagctcggct gcgggtacgg
     4801 atagcgcggg tgcggccgcg ctgcgagcgc gtgcagcagg cggaacaccg cgcgcagcgc
     4861 gacgtactcg ggatccagca ggtccttgtt gcgcagccgc tcgctcggct tcggcaccgc
     4921 ggtgacaatg ctgccgtagt cgatgccggg gcgatcaatc acggccgcgt tgaacagatc
     4981 gatcccctcc ggcgtcaact gcagcaccgc gccctggtcg cgctcgattt cgccgagata
     5041 gcgccacacc gggtcgcggc ggttgaaccg gatccgccgc agcagccctt ccgcgatgcg
     5101 atcgagcgga ccctcggtgc gccccagcca gtcatcgagc cgcgcgacga gagcgatggc
     5161 cttcgcgacc gcgaggatcg cgccgcgccc ctgccccgac gtcgccagcg cgctggcgac
     5221 cagcagcagc gtctgcccct ggatcgtcgc gaagtggttg ccgagcggcg tgccgtggtg
     5281 cggcgtcgcc acgccgatcg cggtccgcgt ccggcggccg atgcgctctg ccgtgccgtc
     5341 gcccccgatc tgcacgcccg gcgtcagcag catgcgcacg tcgaggcccc cggtcgagtg
     5401 accgacgaag tgcagttccc gcgctgcgag tccgccgctg cgaatcacct gccggcgcag
     5461 cgcgtcggta cggcgcggga tcgacgcggt cggctgcgtc atgcagcgga cgatctgcgc
     5521 cgctgcaccg tgctcgcgca gtgcgcggtc gagcacctgc tcgacgcgct cgaaatagct
     5581 gaccgaaccg accgacgtga agccgaagaa gcccggcacc aggaacacga gccgcgtggc
     5641 catgcggtga ttgtgccgct cgcttggcgt gcggcacccg aggcgggtca agtcggcgcg
     5701 cgaaagtcgt gcacctcgat cgccaccggc gtcgggcacg gccggcagcg cagccgtcac
     5761 gcatcgcttg ccagccggcg caactcgtcg agaaaaggcg cctgcccctt ctggatcttc
     5821 gcggccgcct cgtccagcgt gaaccacgcg gcgcgatcca cctcgggaaa ttcacgccgc
     5881 tgccctgacc gcggcggcca ctcgagcgca aacgtgttgc tggtcaactg cgccggatcg
     5941 acgtcggcgc gcagcgcgaa cgcgtggatc accttgccgc tcggttgccg caccggcgcc
     6001 agcgcgacga attcgccgtc caccgatatg ccggtctcct cctcgcactc gcgccgcgag
     6061 gcgagcaa
//
LOCUS       DBNHBY010000403         4133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1648518, whole genome shotgun sequence.
ACCESSION   DBNHBY010000403 DBNHBY010000000
VERSION     DBNHBY010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1648518"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..603)
                     /gene="rplQ"
                     /locus_tag="AC16HB_10075"
     CDS             complement(205..603)
                     /gene="rplQ"
                     /locus_tag="AC16HB_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR5607177.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMSNSLLKHELIKTTLPKAKEL
                     RRVIEPLITLGKEPTLANKRLAFNRLRDREMVVKLFNEIGPRFKSRNGGYTRILKFGF
                     RVGDNAQMALMELVDRPQPVEAAAAEEAQP"
     gene            complement(680..1660)
                     /locus_tag="AC16HB_10080"
     CDS             complement(680..1660)
                     /locus_tag="AC16HB_10080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR5607178.1"
                     /translation="MNSTALLKPRAIEVESIGPNSAIVTMEPFERGYGHTLGNALRRI
                     LLSSMVGYAPTEAQIAGVVHEYSTIDGVREDVVDILLNLKGIVFKLHNRDEVSLTLKK
                     EGEGVVTAADIELPHDVEIVNPDHVIAHLSGGGRLEMQIRVEKGRGYVPGNVRAFRED
                     HSKTIGRIVMDASFSPVKRVAYTVESARVEQRTDLDKLVMTIETNGSITPEEAVRQSA
                     RILVEQLSVFAALEGVAEPITATRVSPQVDPILLRPVDDLELTVRSANCLKAENIYYI
                     GDLIQRTENELLKTPNLGRKSLNEIKEVLAARGLTLGMKLENWPPAGLEK"
     gene            complement(1812..2435)
                     /gene="rpsD"
                     /locus_tag="AC16HB_10085"
     CDS             complement(1812..2435)
                     /gene="rpsD"
                     /locus_tag="AC16HB_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR5607179.1"
                     /translation="MARHTGAKLKLSRREGTDLFLKSARRALDTKCKADSKPGQHGRT
                     SGARTSDYGLQLREKQKVKRMYGVLERQFRRFFAEAERRKGNTGENLIALLESRLDNV
                     VYRMGFGSTRAEARQLVSHCAIAVNGEVVNVPSVWIKPGDVISVRDKAKKQNRVTEAL
                     SLAEQSGFPSWVDVDPKKMEGKFKALPDRGDVAQDVNESLVIELYSR"
     gene            complement(2535..2945)
                     /gene="rpsK"
                     /locus_tag="AC16HB_10090"
     CDS             complement(2535..2945)
                     /gene="rpsK"
                     /locus_tag="AC16HB_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR5607180.1"
                     /translation="MARNPTAQQIAAQRVRKKVKKNVAEGIAHIHASFNNTIITITDR
                     QGNALSWASSGGAGFKGSRKSTPFAAQVAAEAAGRAALECGVKNLEVRIKGPGPGRES
                     SVRALNACGFRITSIQDITPVPHNGCRPPKRRRV"
     gene            complement(2964..3335)
                     /gene="rpsM"
                     /locus_tag="AC16HB_10095"
     CDS             complement(2964..3335)
                     /gene="rpsM"
                     /locus_tag="AC16HB_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR5607181.1"
                     /translation="MARIAGINIPPNQHAEIGLTAIYGIGRARARAILEAVNVPFTKK
                     VKELTDGELERIRDALGKVAVEGDLRREIQLSIKRLIDLGCYRGVRHRKGLPVRGQRP
                     RTNARTRKGPRKDGVALKKPV"
     gene            complement(3348..3461)
                     /gene="rpmJ"
                     /locus_tag="AC16HB_10100"
     CDS             complement(3348..3461)
                     /gene="rpmJ"
                     /locus_tag="AC16HB_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR5607182.1"
                     /translation="MKVMASVKKMCRKCKVIKRKGVVRVICSDPRHKQRQG"
     gene            complement(3478..3696)
                     /gene="infA"
                     /locus_tag="AC16HB_10105"
     CDS             complement(3478..3696)
                     /gene="infA"
                     /locus_tag="AC16HB_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5607183.1"
                     /translation="MAKDDVIQMQGEILENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            complement(3730..>4133)
                     /locus_tag="AC16HB_10110"
     CDS             complement(3730..>4133)
                     /locus_tag="AC16HB_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5607184.1"
                     /translation="PLYILLYASLIIFFCFFYTALVFSSKETADNLKKSGAFVPGIRP
                     GDQTARYIDKILMRLTLIGAIYITLVCLVPEFLHLRFNVPFYFGGTSLLIVVVVTMDF
                     MSQVQAYLMTHQYESLLKKANFKGFGPGLPR"
BASE COUNT          649 a         1353 c         1267 g          864 t
ORIGIN      
        1 gagaagactt ggctcgcgca cggtggccct cgcagattgt cgcagtcgcg tggcgcgact
       61 gcgtgcgggc tcggccgccg cttcctgctg cttgggctga cccgatccac caccttcttc
      121 agcgccatcc gcgcattgcg gcttcagtcg gcgttgcgcg cctcacattc agcggcgctc
      181 aagtcggcct gcgacgcaac gcgcctaagg ttgcgcctct tccgccgcgg ccgcttcgac
      241 gggctgtggg cgatccacca gttccatcag cgccatctgc gcgttgtctc cgaccctgaa
      301 cccgaacttg aggatgcgcg tgtagccacc attgcgcgac ttaaagcgcg gtccgatttc
      361 gttgaagagc ttgaccacca tctcgcggtc gcgcagacgg ttgaaggcaa gccgcttgtt
      421 cgcgagggtg ggctccttac ccagcgtgat cagcggctcg atcacacgcc gcagttcctt
      481 cgccttcggc agcgtggtct tgatcagctc gtgcttgagc agggagttcg acatgttgcg
      541 cagcatcgcc aggcgatggc tgctggtgcg gttcagtttc cgcagtccgt gacggtgacg
      601 catcattctt cctttcagtc ttggatgggt cggctcttct atcccgcagg cgggccgact
      661 tgcttgcgcg ctggtgcgct tacttctcca aacccgctgg cggccaattc tcgagcttca
      721 tcccgagcgt cagtccgcgc gccgccagca cttccttgat ctcgttcagt gacttgcggc
      781 cgaggtttgg ggtcttcagt agttcgttct cggtgcgctg gatcaggtcg ccgatgtagt
      841 agatgttctc ggccttcagg cagttggccg agcgtaccgt gagttccaga tcgtcgaccg
      901 gtcgcagcag aatcggatcg acctgtggcg agacgcgcgt ggcggtgatt ggctcggcca
      961 cgccttccag cgcggcgaac actgacagct gctcgaccag gatgcgggcc gattgccgca
     1021 ccgcctcctc cggcgtaatc gaaccgttcg tctcgatcgt catcaccagc ttgtcgaggt
     1081 cggtgcgctg ctcgacccgt gcgctctcga ccgtgtatgc cacgcgcttg accggcgaga
     1141 acgacgcgtc catcacgatg cggccgatgg tcttgctgtg gtcctcgcgg aatgcgcgaa
     1201 cgttgcccgg cacgtagccg cggccctttt cgacgcgaat ctgcatctcc agccgcccgc
     1261 cgcccgacag gtgcgcgatc acgtggtcgg gattgacgat ctcgacatcg tgcggcagct
     1321 cgatgtcggc cgcggtgacg acaccctcgc cttccttctt gagcgtcagg gaaacctcgt
     1381 cacggttgtg cagcttgaag acgattccct tcaggttcag cagaatgtcg accacgtcct
     1441 cgcgcacgcc atcgatggtc gagtattcgt gcacgacgcc ggcaatctgc gcctcggttg
     1501 gcgcgtagcc gaccatcgac gacagcagga tgcgccgcag cgcgttgccg agcgtatggc
     1561 cgtagccacg ctcgaagggc tccatcgtga cgatggcgga attgggaccg atcgattcga
     1621 cttcgatcgc ccgcggtttc agcaatgcgg ttgagttcat tgacgatcct tttcaatacc
     1681 ctcggctcgt tacgccgata aggctggtgg atggtccggg gcgggtttgg cggcggctgt
     1741 tgcccccagg gcagccgctg gttgtccgtt gctcgttgtc cgttcagcga gcaacggaca
     1801 acggacaaag atcaacgtga atacaactcg atgacgagcg actcgttcac gtcctgcgcg
     1861 acgtcgcccc ggtccggcag cgccttgaac ttgccttcca tcttcttcgg atcgacatcg
     1921 acccatgatg gaaatccgga ctgctcggcg agcgaaaggg cctcggtgac gcggttctgc
     1981 ttcttggcct tgtcgcgcac gctgatcacg tcgccgggct tgatccacac cgatggcacg
     2041 ttgacgactt cgccgttgac cgcgatcgcg caatgcgaca ccagttggcg cgcctcggcc
     2101 cgggtcgaac cgaagcccat ccggtacacg acgttgtcca ggcgcgactc cagcagcgcg
     2161 atcagattct cgccggtgtt gcccttacgg cgctcggcct cggcgaagaa gcggcggaac
     2221 tgccgctcca gcacgccgta catgcgcttg accttctgct tctcgcgcag ctgcagcccg
     2281 tagtccgacg tgcgggcgcc cgaggtgcgg ccgtgctggc cgggcttcga atcggccttg
     2341 cacttggtgt cgagcgcgcg ccgcgcgctc ttcagaaaca gatccgtgcc ttcgcgacgc
     2401 gaaagcttga gttttgctcc ggtgtggcga gccatcttcg tttacctatc ctgcagcgtg
     2461 ctgcattaag tccgcgttca gtgcgcggac accagattgt cgagacccat cgtcagtcga
     2521 tcccccgaaa aaaatcagac acgccggcgc ttgggcgggc ggcagccgtt gtgcggcacc
     2581 ggcgtgatgt cctggatcga cgtgatccgg aacccgcacg cgttcagcgc ccgtacgctc
     2641 gactcgcggc cggggccggg gcccttgatc cgcacctcga ggttcttcac cccgcactcc
     2701 agcgcggcgc ggccggccgc ctcggcggcc acctgcgccg cgaacggcgt ggacttgcgc
     2761 gagcccttga agccggcgcc gccgctggat gcccatgaca gtgcattgcc ctggcggtcg
     2821 gtgatcgtga tgatcgtatt gttgaacgac gcgtgaatgt gcgcgatgcc ctcggcgacg
     2881 ttcttcttga ccttcttgcg cacccgctgg gcggcaatct gctgcgcggt cggattcctt
     2941 gccatgttct gtgtcctcgt gccttagacc ggtttcttca gcgctacgcc atccttgcgc
     3001 ggccccttgc gcgtacgcgc attggtgcgc gggcgctggc cgcgcaccgg caggcccttg
     3061 cgatgacgca cgccgcgata gcagcccaag tcgatcagcc gcttgatcga cagttggatt
     3121 tcgcgccgca ggtcaccctc caccgcgacc ttgccgagcg catcgcggat gcgctcgagt
     3181 tcgccgtcgg tcagttcctt caccttcttg gtgaacggca cgttgaccgc ctcgaggatc
     3241 gcgcgcgcgc gggcgcggcc gatgccgtag atcgcagtca ggccgatctc ggcgtgctgg
     3301 ttgggcggaa tgttgatacc tgcaattcgg gccattgtcg tccctcgtca gccttgacgc
     3361 tgcttgtggc gcgggtcgga gcagatcacg cgcaccacgc ccttgcgctt gatcaccttg
     3421 cacttgcggc acatcttctt gacgcttgcc atgactttca tggttctctc caccgcgcta
     3481 cttggcgcgg aaaacgatcc ttgctcgact caggtcgtac ggcgtcagtt ccaccgtcac
     3541 cttgtcgccc ggcaggatgc ggatgtagtg catccgcatc ttcccggaga tgtggccgag
     3601 caccacatgc ccgttctcca gcttcacgcg aaacgtcgcg ttcggaagat tctcgagaat
     3661 ctccccctgc atctggatga cgtcgtcttt cgccatgcgc tcctttggcc cgctgcgtct
     3721 ggtcaacccc tagcgcggca ggccggggcc gaaacccttg aagttcgcct tcttcagcaa
     3781 agattcgtac tgatgcgtca tcaggtacgc ctgcacctgc gacatgaagt ccatcgtcac
     3841 gaccacgacg atcagtagcg acgtgccgcc gaaatagaac gggacgttga agcgcaggtg
     3901 caggaactcc ggcaccaggc acaccagcgt gatgtagatg gcaccgatca gcgtcagccg
     3961 catcaggatc ttgtcgatat agcgcgcggt ctgatcgccc gggcggatgc ccggaacgaa
     4021 cgcgccgctc ttcttcaggt tgtccgccgt ttccttgctg ctgaacacga gcgccgtgta
     4081 gaagaagcag aagaagatga tcaggctcgc gtaaagcagg atgtacagcg gct
//
LOCUS       DBNHBY010000404         3055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_497714, whole genome shotgun sequence.
ACCESSION   DBNHBY010000404 DBNHBY010000000
VERSION     DBNHBY010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_497714"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="AC16HB_10115"
     CDS             <1..1227
                     /locus_tag="AC16HB_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HIR5607185.1"
                     /translation="PLGPLQLQICSLDYSSYVGKIGIGRITRGRIRSGQEVVVLNGPD
                     ALPIRGRINQVLKFKGLERELVDEALAGDIVLVNGIDELGIGSTICPVDAPDALPLLR
                     VDEPTLMMNFCVNTSPLAGREGKYVTSRQLRERLERELKSNVAMRMKETSDETIFEVA
                     GRGELHLTILIENMRREGYELAVSRPRVLLKEIDGIRHEPYELLTVDIEEQHQGAVME
                     ELGQRKGDLQDMQSDGRGRVRLEYRIPARGLIGFQGEFLTLTRGTGLMSHIFDDYAPV
                     KPGDVGSRRNGVLVSQDDGEAVAYALWKLQERGRMFVSPGDKLYEGMIIGIHSRDNDL
                     VVNPIREKKLTNVRAAGKDEHIDLVPPIRLTLEYAVEFIADDELVEVTPQSIRLRKRY
                     LKEHERKRAEREKELA"
     gene            1224..2150
                     /locus_tag="AC16HB_10120"
     CDS             1224..2150
                     /locus_tag="AC16HB_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607186.1"
                     /translation="MSVEVTARAHRRALLIMIVVPMLWSTAGVVTRHLSPELQAQGRF
                     EITFWRSLFAALTVAVALAHFRREGFRASFATGLPGLVSGAMWAVMFSAFMLALTLTT
                     TANTLLVLSLAPLVTAILARLVLRVRIATRTWIAIVAAMIGMGWMFSQDAGGGPTGNH
                     LLGMLIAFSVPLASAINLVTLQKTRAQVDLVPSVLLGGAISAALMLPFALPFRAAPTD
                     VLLLAALGIFQLGVPCMLMVIATRSLSAPEVSLLALLEVVLGPLWAWLGAGETPSAST
                     LYGGAIVLAALIGNELLGLGSATVTARATTTT"
     gene            complement(2143..>3055)
                     /locus_tag="AC16HB_10125"
     CDS             complement(2143..>3055)
                     /locus_tag="AC16HB_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607187.1"
                     /translation="ALTACRIQIEGIRERLATEHRGIVTGAAATSLRAHLQVAPEVAA
                     RFHNASMIAAFATAGLAANSPYLFGCELWEDTRIALLEERTAPPPQFIATVEDYFRAN
                     LEQPTVTVAATDPPQRFTQLRRHNEGLPRFTHLQVGLDADGPPHLRIEQRAMSPGPTP
                     ADMLANLAFHYGLTASLATEPDPPEERIDAASAHANLLAVARGGLNTEIAWLDRERMP
                     LRTLTIVELIERAYEGLHQLGVDEGDAQRHLTLIERRVANGQTGAVWQRRFVQAYGRN
                     FALLAREYAARQLGGAPVHEWNLRRLT"
BASE COUNT          458 a         1004 c         1089 g          504 t
ORIGIN      
        1 ccgctcggtc cgctgcagct gcagatctgc tcgctcgatt attcgagcta tgtcggcaag
       61 atcggcatcg gacgcatcac gcgcgggcgc atccgctccg gccaggaagt cgtcgtgctg
      121 aacggcccgg acgcgctccc gatcaggggg cgcatcaacc aggtgctgaa gttcaagggg
      181 ctggagcgcg aactggtcga cgaggcgctg gccggcgaca tcgtgctcgt caacggcatc
      241 gacgagctcg gcatcggctc gacgatctgc ccggtcgacg cgcctgacgc tctgccgctg
      301 ctgcgcgtcg acgagccgac gctgatgatg aacttctgcg tgaacacgtc gccgctggcg
      361 ggccgcgagg gcaaatacgt gaccagtcgc cagttgcgcg agcggctgga gcgtgaactg
      421 aagtccaacg tggcgatgcg gatgaaggag accagcgacg agaccatttt cgaggtcgca
      481 ggccgcggcg agctgcatct gacgatcctg atcgagaaca tgcgccgcga gggctacgag
      541 ctcgcagtgt cgcgtccgcg cgtgctgctc aaggagatcg acggcatcag gcatgagccg
      601 tacgaactgc tgactgtcga catcgaggag cagcaccagg gcgcggtgat ggaggagctc
      661 ggccagcgca agggcgatct gcaggacatg cagtccgacg gccgcggccg cgtgcggctg
      721 gagtaccgca tcccggcgcg cgggctgatc ggcttccagg gcgagttcct gacgctgacg
      781 cgcggcaccg ggctgatgag ccacatcttc gacgactacg cgccggtgaa gccgggcgac
      841 gtcggcagcc gtcgcaacgg cgtgctggta tcgcaggacg acggcgaggc ggtcgcctac
      901 gcgctgtgga agctgcagga acgtggccgc atgttcgtca gccccggcga caagctttac
      961 gagggcatga tcatcggcat ccattcgcgc gacaacgacc tcgtcgtcaa cccgatccgt
     1021 gagaagaagc tgaccaacgt acgcgcggcg ggcaaggacg agcacattga cctggtgccg
     1081 ccgatccggc tcacgctcga gtacgcggtc gagttcatcg ccgacgacga gctggtcgag
     1141 gtcacgccgc agagcatccg cctgcgcaag cggtatctga aggagcacga gcgcaagcgc
     1201 gccgagcggg agaaagagct ggcgtgagcg tagaggtgac cgcgcgcgcg catcggcgcg
     1261 cactgctgat catgatcgtg gtgccgatgc tgtggtcgac cgcaggcgtg gtgacgcgcc
     1321 acctgtcgcc ggagctccag gcgcagggtc gcttcgagat cacgttctgg cgcagcctgt
     1381 tcgcggcgct gaccgtcgcg gtggcgctcg cgcacttccg ccgcgaaggt tttcgcgctt
     1441 cgtttgcgac cgggctgccg ggcctggtgt cgggcgcaat gtgggcggtg atgttcagcg
     1501 ccttcatgct ggcgctgaca ttgacgacga cggccaacac gctgctggtg ctcagcctcg
     1561 cgccgctggt cacggcgatc ctggcgcggc tggtgctgcg ggtgcggatc gcgacgcgca
     1621 catggatcgc gatcgttgcc gcgatgatcg gcatgggctg gatgttctcg caggacgccg
     1681 gcggcggtcc gaccggcaat catctgctcg gcatgctgat cgcgttctcg gtgccgctcg
     1741 cctcggcgat caacctcgtc accctgcaga agacgcgcgc gcaggtcgac ctcgtcccct
     1801 cggtgttgct cggcggcgcg atttccgcgg ctctgatgct gccgttcgcg ctgccgtttc
     1861 gcgccgcgcc gaccgatgtc ctgctgctgg cggcgctggg catcttccag ctcggcgtgc
     1921 catgcatgtt gatggtcatt gccacgcgca gcctgtcggc acccgaggtt tcgctgctcg
     1981 cgcttctgga ggtcgtactc ggcccgctct gggcgtggct cggtgccggc gagacgccgt
     2041 cggcctcgac gctctatggc ggcgcgatcg tgctggccgc gctgatcggc aacgagctgc
     2101 tcggcctggg cagtgcgacg gttaccgcgc gcgcgacgac gactacgtaa gccgccgcag
     2161 gttccactcg tgcaccggcg caccgcccaa ctggcgcgcc gcatattcac gcgccagcag
     2221 agcgaagttg cgcccgtacg cctgcacgaa gcggcgctgc cacaccgcgc cggtctgacc
     2281 gttggccacg cgacgctcga tgagcgtcag atgtcgctgc gcgtccccct cgtcgacacc
     2341 gagctggtgc agtccctcgt acgcacgctc gatcagctcg acgatcgtca gcgtgcgcag
     2401 cggcatgcgc tcgcgatcga gccatgcgat ctcggtgttg agtccgccgc gcgcgaccgc
     2461 gagcaggttg gcgtgcgcgc tggccgcgtc gatgcgctct tcaggcggat cgggttcggt
     2521 cgcgagcgac gcggtcagcc cgtagtgaaa ggcgaggttc gccagcatgt ccgccggtgt
     2581 cggtcccggc gacatcgcgc gctgctcgat ccgcaggtgc ggcggtccgt ccgcgtcgag
     2641 accgacctgc aggtgcgtga agcgcggcag tccctcgttg tgccggcgca gttgcgtgaa
     2701 gcgctggggc gggtcggtgg cggcgaccgt cacggtcggc tgctccagat tggcgcggaa
     2761 gtagtcctcc accgtcgcga tgaactgcgg cggcggcgcc gtgcgctctt cgagcagggc
     2821 gatccgggtg tcctcccaca gctcgcaacc gaacagatac ggagagttgg ccgccagacc
     2881 cgcggtcgcg aaggcggcga tcatcgacgc gttgtggaag cgcgccgcga cttcgggcgc
     2941 cacctgcaga tgcgcgcgca gcgaggtggc cgccgcgccg gtgacgatgc cgcgatgctc
     3001 ggtcgccagc cgctcgcgga tgccttcgat ctggatccgg cacgcggtca gcgcg
//
LOCUS       DBNHBY010000405         2703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_835612, whole genome shotgun sequence.
ACCESSION   DBNHBY010000405 DBNHBY010000000
VERSION     DBNHBY010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_835612"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="AC16HB_10130"
     CDS             complement(<1..896)
                     /locus_tag="AC16HB_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR5607188.1"
                     /translation="MSIARRRLTESARSLDGARGLLGQRPVVYSGNRVRLLVNGDEYF
                     PALLAAIEEARSTIHLETYIYADDKIGIRVTDALAAAAARGVQVRLLIDGFGGGEYAR
                     RLVKGLGVHGAEVRIFRPERWWRLERKLLRRLHRKIAVIDDRLAFVGGINIIDDHNVP
                     PAERGRIGPRFDFAVACQGPVVAPIALAARQLWWMLSIVRPTQPSGPRPRIDLQAQPA
                     SFPGGVAASFLLRDNLRHRRTIERAYLDAIESARQDIVIASAYFIPGRRVRAALLAAA
                     ARGVRVRLLLQGRVEYAIQHFA"
     gene            complement(893..1705)
                     /locus_tag="AC16HB_10135"
     CDS             complement(893..1705)
                     /locus_tag="AC16HB_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5607189.1"
                     /translation="MKLKVATYNIHKGVTGIRRRPRIHDVRHALHAISADIVFLQEVQ
                     DRNERLRHHANYPTTNQLDFLATTGYEHQVYGKNAIYPHGHHGNAILSRHPIRHSTNH
                     DISDHALEKRGMLHAVTRVPGPGKSPPADVHLICVHFGLIKRSRLRQADLLAAFVERE
                     IPRKSPLIIAGDFNDWQRRVDELLRARLRVEEVAVAREAPRRSVLDKLLPWRDARAAQ
                     PPARTFPSFAPMLTLDRIYVRGFRVAGMQVPKGLTWARRSDHAPLIAELVLR"
     gene            1765..>2703
                     /locus_tag="AC16HB_10140"
     CDS             1765..>2703
                     /locus_tag="AC16HB_10140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR5607190.1"
                     /translation="MPLTRRSLLRLAVAAAAPGAWTALPRAQPLSRDPFTLGVASGPA
                     AHGVVLWTRLAPDIDADIARLSHLGTESYVAARRDELQRPAASYDVRWEVAEDESFGK
                     VVRRGTARALRELGASVHVELGDLPPARWYFYRFLLGDAVSPVGRTRSPDAPGSPRTR
                     LRFALASCQHYEHGTFGAYAQMRGDDPELVLFVGDYIYEGGPVAGRFRPHPFPSARTL
                     FDYRLRHALYRRDSSLQQMHAHCPWLVTWDDHEVSNDYAGDVGEDPAVDGHARRLAAY
                     QAYYEHMPLSVATLVERFSHVRLYRTHRAARLASFVV"
BASE COUNT          382 a          945 c          955 g          421 t
ORIGIN      
        1 tgcgcaaagt gctggatcgc atattcgacg cgcccctgca gcagcagccg gacccgcaca
       61 ccgcgggcgg ccgcggcaag cagcgcggcg cgcacgcgcc ggccggggat gaaatacgcg
      121 ctggcgatca cgatgtcctg acgtgcgctc tcgatcgcgt ccaggtatgc gcgttcgatc
      181 gtgcggcgat gccgcaggtt gtcgcgcagc aggaacgacg ccgcgacgcc gcccgggaac
      241 gacgccggct gcgcctgcaa gtcgatgcgc ggccgcgggc ccgatggctg ggtcggacgc
      301 acgatggaca gcatccacca aagctgcctg gccgccagcg cgatcggcgc cacgactggt
      361 ccctgacacg cgaccgcgaa atcgaagcgc gggccgatgc gcccgcgctc ggccggcggc
      421 acgttatggt cgtcgatgat gttgatgccg ccgacgaacg ccagccggtc gtcgatcacc
      481 gcgatcttgc gatgcaggcg gcgcagcagc ttgcgctcca gccgccacca gcgctcgggg
      541 cggaagatcc gcacttccgc gccatgcacg ccgagtccct tgaccagccg ccgcgcgtat
      601 tcgccgccgc cgaaaccatc gatcagcaac cgcacctgga cgccgcgcgc cgccgccgcg
      661 gccagcgcgt cggtgacgcg gatgccgatc ttgtcgtccg cgtagatgta ggtctccagg
      721 tggatcgtgc tgcgcgcctc ctcgatcgcg gccagcagcg ccgggaagta ctcgtcgccg
      781 ttgaccagca gccgcacgcg gttgcccgaa tagacgaccg gccgctggcc gagcagaccg
      841 cgcgcgccgt cgagcgagcg cgccgactcg gtcagccggc gtcgcgcaat gctcatctga
      901 gcaccagctc cgcgatcaac ggcgcgtgat ccgagcggcg ggcccaggtc agccccttgg
      961 gtacctgcat gccggcgacg cggaagccgc gtacgtagat gcggtcgagc gtcagcatcg
     1021 gtgcgaagct ggggaacgtg cgcgccggcg gctgcgccgc acgcgcgtcg cgccacggca
     1081 gcagcttgtc gagcacgctg cggcggggcg cctcccgcgc caccgcgacc tcttcgacac
     1141 gcagccgcgc gcgcagcaac tcgtcgacgc ggcgctgcca gtcgttgaag tcgccggcga
     1201 tgatcagcgg cgacttgcgt gggatctcgc gctcgacgaa ggcggcgagg aggtcggcct
     1261 gccgcaggcg actgcgcttg atcaggccga agtgcacgca gatcaggtgc acgtccgccg
     1321 gggggctctt gcccgggccg ggcacgcgcg tgaccgcgtg caacatgccg cgcttttcca
     1381 acgcatggtc cgaaatgtcg tggttggtcg agtgccggat cgggtggcgc gacaggatcg
     1441 cgttgccgtg atggccgtgc ggatagatcg cgttcttgcc gtacacctga tgctcgtagc
     1501 ccgtcgtggc caggaagtcg agctggttcg tggttgggta gttggcgtga tggcgcagcc
     1561 gctcgttgcg gtcctgcact tcctgcagga acacgatgtc ggcgctgatc gcatgcagcg
     1621 catggcgcac gtcgtggatg cgcggccggc ggcggatgcc ggtcacgccc ttgtggatgt
     1681 tgtaggtggc gactttcagc ttcataccgc cgaatacgat accgtctgct ccgcgacgca
     1741 gtgctgacat ttcgtgggat gacgatgccg ttgacccgac gttcgctgct gaggcttgcc
     1801 gtggccgccg ccgcgccagg cgcgtggacc gcgctgccgc gcgcgcagcc gctcagccgc
     1861 gatccgttca cgctcggcgt ggcgagcggg ccggccgcgc atggcgtggt gctgtggacg
     1921 cggctcgcgc ccgacatcga cgccgacatc gcccgcttgt cgcacctcgg cacagagtcg
     1981 tacgtcgccg cccgccgcga cgagctgcag cggccggcgg cgtcgtacga cgtgcgctgg
     2041 gaggtggccg aagacgagtc gttcggcaag gtggtccggc gtggcacggc gcgcgcgctg
     2101 cgcgaattgg gcgcaagcgt gcacgtggag ctcggcgacc tgccgcccgc gcggtggtac
     2161 ttctaccgtt tcctgctcgg cgatgcggta agcccggtcg gacgcacgcg ctcgcccgat
     2221 gcccccggat caccgcgcac gcggctgcgc ttcgcgctgg cgtcgtgcca gcactacgag
     2281 catggcacct tcggcgccta cgcgcagatg cgcggcgacg acccggagct ggtgctgttc
     2341 gtcggtgact acatctacga gggcggtcct gtcgcgggcc gtttccgtcc gcacccgttt
     2401 ccttcggcgc gcacgttgtt cgactaccgg ctgcgccacg cgctgtaccg gcgcgactcg
     2461 agcctgcagc agatgcatgc ccactgcccg tggctcgtga cctgggacga ccatgaagtg
     2521 tcgaacgact acgcgggcga cgtcggcgag gaccccgccg tcgacggcca cgcgcggcgg
     2581 ctggccgcgt accaggcgta ctacgagcac atgccccttt cagtggccac cctcgtcgag
     2641 cgcttttcgc acgtgcggct gtaccgcacg catcgagccg ctcggctcgc gtcattcgtc
     2701 gtg
//
LOCUS       DBNHBY010000406         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1513158, whole genome shotgun sequence.
ACCESSION   DBNHBY010000406 DBNHBY010000000
VERSION     DBNHBY010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1513158"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..>1887
                     /locus_tag="AC16HB_10145"
     CDS             277..>1887
                     /locus_tag="AC16HB_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5607191.1"
                     /translation="MSEPVNAQTIADHRGQRVSNLKLLFVHSRVGRRLMSLFLLSALV
                     PVIVLGASLFARMSDFLRESNTAELAQAARTFGDVTLERLRRIDAELRTLERVPADRS
                     PERWAVTQEGQLSRAVKALALLDSEGRVVRSRGAIPDASYLRGLAGAGDARQSGLATR
                     TDQAAAEILLIHPFLAAAGRYVIVAKLDPELLWSDDRPAGAPFDHCVTTASGARLHCS
                     RPALELPTTLLSARALEAPTTVNWTNGDEPLQALAVALPLRNPAANEDWTVTAIRSDR
                     AALQTDRSFRISVAIVAVLSVLLALALSLNQMRRILVPLERLLLGAQSVAENDFSVRI
                     AASLQDEFGRVAHGFNAMAERLGLQFHTMQAFSEIDRTILVSPDIGKICKLALQALKG
                     ITGAEVLSLGAAEPGSPGQLRIYTFAADGAELAPGRTIAGNPDPKLFSGPAAVWDWQE
                     HPLLPNGYLPALRPVNSSVYLVQPIVRNRRVWGLIVLGHSESVASSPRRRALIRDVAD
                     RVAVALATVERDRRLHVMAHLDGLTGLPN"
BASE COUNT          283 a          641 c          670 g          293 t
ORIGIN      
        1 cctgcgaagg agacgcgcga ggagcctgcc ggcctgctgg gaacgctgcg cagcgcgttg
       61 ggcctggcgc gacgctaggg tgcagtcaga gctgccaccg atcggcccgg cggtcaccat
      121 gtgcaaccga ttcgtgtgga cgagacgcaa ccgaaggacg gacgaccgat ccggacaggg
      181 tgcatccccg cgccgagcac ggggggctag gctgttcttc ccttcgcaac gtctggcgct
      241 gccgcatggc ggcagccggc gtcggcacga caaagcatga gcgaaccggt caacgcacag
      301 acgatcgcgg atcatcgcgg gcagcgggtg tcgaacctga agctgctgtt cgtgcacagc
      361 agggtcggac gccggctgat gtcgctgttc ctgctttccg cattggtgcc ggtgatcgtg
      421 ctcggggcat ccctgttcgc tcgcatgagc gacttcctgc gcgagtccaa caccgccgaa
      481 ctcgcgcagg cagcgcggac ctttggcgac gtcacgctgg agcggctgcg tcgcatcgat
      541 gccgagctgc gcacgctgga acgggtaccg gcggatcgct cgccggagcg ttgggccgtg
      601 acacaggaag gccagctcag ccgcgccgtc aaggcgcttg cgctcctcga cagcgagggg
      661 cgcgtcgtgc ggtcgcgcgg cgcgatcccc gacgcgtcgt acctgcgcgg actcgccggc
      721 gccggcgatg cgcgtcaatc cggcctcgcg acgcgcaccg accaggcggc ggccgagatc
      781 ctgctgatcc atccgttcct ggccgcggcg ggacggtacg tcatcgtcgc caaactcgac
      841 cccgagttgc tgtggagcga cgaccggccg gccggcgcac cgttcgatca ttgcgtcacc
      901 accgcgagtg gcgcgcgcct gcactgctcg cgacccgcgc tcgagttgcc gacaacgctt
      961 ctgtccgcgc gcgcgctcga ggcgccgacg acggtgaact ggaccaacgg cgacgagccg
     1021 ctgcaggcgc tggcggtcgc gctgccgctg cgcaatccag cggcgaacga ggactggacc
     1081 gtcacggcaa tccgctcgga ccgcgccgcg ctgcagaccg atcggtcgtt tcgcatcagc
     1141 gtggcgatcg tcgcggtgct cagtgtgctg ctggcactgg cgctcagcct gaaccagatg
     1201 cgccgcatcc tggtgccgct cgaacgcctg ctgctcgggg cgcagagcgt ggccgagaac
     1261 gacttttcgg tgcgcatcgc ggcgtcgctg caggacgaat tcggacgcgt ggcgcacggc
     1321 ttcaacgcga tggccgaacg gctcggactg cagttccaca cgatgcaggc gttctccgag
     1381 atcgatcgca cgatcctcgt gtcgccggac atcggcaaga tctgcaagct cgcgctgcag
     1441 gctctgaagg gaatcacggg cgccgaggtg ctgagcctgg gcgcggccga gcccggcagt
     1501 cccggtcaac tgcggatcta cacctttgcc gccgacgggg ccgagctggc gccgggcagg
     1561 acgatcgccg ggaatccgga tccgaagctc ttcagcggcc ccgcggcggt gtgggactgg
     1621 caggagcatc cattgttgcc caatggatac ctgccggcgc tgcgccccgt gaactcgtcg
     1681 gtgtatctgg tgcagccgat cgtgcgcaat cggcgcgtgt gggggttgat cgtgctcggc
     1741 cacagcgagt cggtggcctc gtcgccgcgc cgacgcgcgc tgatccggga cgtcgccgat
     1801 cgcgtagccg tcgcgctggc caccgtcgag cgcgaccggc gcctgcacgt gatggcgcat
     1861 ctggacggtc tgaccggatt gccgaac
//
LOCUS       DBNHBY010000407         1943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1108008, whole genome shotgun sequence.
ACCESSION   DBNHBY010000407 DBNHBY010000000
VERSION     DBNHBY010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1108008"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16HB_10150"
     CDS             complement(<1..362)
                     /locus_tag="AC16HB_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR5607192.1"
                     /translation="MATQLKYGFGKAVALGFDAAIEKVTQELAKEGFGVLTEIDVQAT
                     LKKKLGVEGPPYRILGACNPPFAHRALTAEPQIGLLLPCNVVVRQDDAGKVLVEFMDP
                     EAVLKLVDRAEVAPIAAEV"
     gene            complement(366..1643)
                     /locus_tag="AC16HB_10155"
     CDS             complement(366..1643)
                     /locus_tag="AC16HB_10155"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5607193.1"
                     /translation="MAHVVVMGAGLGGLPMAYEMKELLRPEDRVTVVGLGASYSFVPS
                     NPWVAVHWRKRSDIEFEVAPYLEKKGIAYDGRGVKRVHPKESRLELNDGVSLAYDYLV
                     IATGPKLAFDEVEGLGPNGHTQSICMVDHAEKGASAWDAFVDAPGPIVVGAVQGASCF
                     GPAYEFCFIMETDLRRRKIRDKVPMTFVTAEPYIGHLGLGGVGDSKSMLESALRDKHI
                     KWICNAKVAKVEPGKMFVTEHDEDGKPKKEHVLPFAYSMMLPAFKGVDAVFGIEGLTN
                     PRGFILIDGHQRNPTHKNIYAVGVCVAIPPVEATPVPTGAPKTGFMIESMVTAAAHNI
                     REQIDGKEPSHKATWNAVCLADMGDTGIAFVALPQIPPRNVNWFSEGKWVHLAKVAFE
                     KYFIRKLKKGTSEPYYEKFVMSALGITKLKEKV"
     gene            complement(1761..>1943)
                     /locus_tag="AC16HB_10160"
     CDS             complement(1761..>1943)
                     /locus_tag="AC16HB_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607194.1"
                     /translation="SLQDVLGELNDIAVARKLLVQFDSAGTQSFRSALEERRAQLVRA
                     GARELAALARTRHPWD"
BASE COUNT          318 a          685 c          571 g          369 t
ORIGIN      
        1 acctcggccg cgatcggggc gacttcggca cggtcgacca gcttcagcac cgcctccgga
       61 tccatgaact cgaccagcac cttgccggcg tcgtcctggc ggacgacgac attgcaggga
      121 aggagcaggc cgatctgcgg ctccgccgtc agcgcgcggt gtgcgaacgg cggattgcat
      181 gcgccgagga tccggtatgg cggcccctcg acgccgagtt tcttcttcag cgtcgcctgc
      241 acgtcgatct cggtcaggac gccgaagcct tccttcgcca gttcctgcgt gaccttctcg
      301 atcgccgcat cgaagccgag cgcgaccgcc ttgccgaaac cgtatttcaa ctgcgttgcc
      361 atgccttaca ccttttcctt cagtttggtg atgccgagcg cgctcatcac gaacttctcg
      421 taatagggct ccgaagtgcc tttcttcagc ttccgtatga agtacttctc gaaagcaacc
      481 ttggcgaggt gcacccactt gccctcgctg aaccagttga cgttgcgcgg cgggatctgc
      541 ggcagcgcca cgaacgctat gcccgtatcg cccatgtcgg ccaggcaaac cgcgttccac
      601 gtcgccttgt ggcttggctc cttgccatcg atctgctcgc ggatgttgtg cgcggcggcc
      661 gtcaccatcg actcgatcat gaagccggtc ttcggcgcgc cggtcggcac cggcgtcgct
      721 tccaccggcg ggatcgccac gcacacgccg accgcgtaga tattcttgtg ggtcgggttg
      781 cgctggtgcc cgtcgatcag gatgaagccg cgcggattgg tgagcccctc gatgccgaag
      841 accgcgtcca cgcccttgaa cgccggcagc atcatcgagt aggcaaacgg cagcacgtgc
      901 tccttcttcg gcttgccgtc ttcgtcgtgc tcggtcacga acatcttgcc gggctcgacc
      961 ttggcgacct tggcgttgca gatccacttg atgtgcttgt cgcgcagcgc ggactcgagc
     1021 atcgatttcg aatcgccgac gccgccgagc ccgaggtgac cgatgtacgg ctccgccgtg
     1081 acaaaggtca tcggaacctt gtcgcggatc ttccgccgcc gcagatccgt ttccatgatg
     1141 aagcagaact cgtacgccgg gccgaagcac gacgcgccct gcaccgcacc gaccacgatc
     1201 ggccccggcg catcgacgaa cgcatcccac gccgacgcac ctttttcggc atggtcgacc
     1261 atgcagatcg actgcgtgtg cccgttcggc cccagcccct cgacctcgtc gaacgcgagc
     1321 ttcggacccg tggcgatcac cagatagtcg tacgcgagac tgacgccgtc gttgagctcc
     1381 aggcggcttt ccttcggatg cacgcgcttc acgccgcggc cgtcgtaagc gatgcccttc
     1441 ttttcgaggt acggcgcgac ctcgaactcg atgtcgctgc gcttgcgcca gtgcaccgcg
     1501 acccagggat tcgacggcac gaagctgtag ctcgcaccca gcccgaccac cgtgacgcgg
     1561 tcttccggcc gcagcagttc cttcatctcg taggccatcg gcagtccgcc gagacccgcg
     1621 cccatcacga ccacatgcgc catgtccgca gtctcctgta tccgtcgccg gcgccgaccg
     1681 ttcgcgcctc gttctcaacc gctaacgtaa gtgcgatacg gagcagggta tgtgacccag
     1741 gtcaccttcg acgacgcggt tcagtcccac ggatgacgag ttcgcgcgag tgcggcaagt
     1801 tcccgcgcgc ccgcgcggac cagctgtgcc cgccgctcct cgagcgcaga gcggaacgac
     1861 tgcgtgccgg cggaatcgaa ctgcaccagc aacttgcgcg cgaccgcgat gtcgttcaat
     1921 tcgcccagca cgtcctgcag cga
//
LOCUS       DBNHBY010000408         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1513306, whole genome shotgun sequence.
ACCESSION   DBNHBY010000408 DBNHBY010000000
VERSION     DBNHBY010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1513306"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="queA"
                     /locus_tag="AC16HB_10165"
     CDS             <1..571
                     /gene="queA"
                     /locus_tag="AC16HB_10165"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR5607195.1"
                     /translation="PDDAADEARYQTVYAKTPGAVAAPTAGLHFTEELLARLQHRRVR
                     FAYVTLHVGAGTFQPVRVEPIADHVMHSEWYAIDADAARIVNDARAAGRRVTAVGTTS
                     LRALESAARDGLVEAGARDTRLFITPGFRFQIVDRLVTNFHLPQSTLLMLVSAFAGME
                     RIRGAYAHAIAQRYRFFSYGDAMLLERAP"
     gene            568..1701
                     /gene="tgt"
                     /locus_tag="AC16HB_10170"
     CDS             568..1701
                     /gene="tgt"
                     /locus_tag="AC16HB_10170"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR5607196.1"
                     /translation="MTARFELLATEGEARRGRLALNHGVVETPVFMPVGTYGTVKAMT
                     PRELDESGAQIVLGNTFHLWLRPGVEVIGRHAGLHRFMGWDKPILTDSGGFQVFSLGA
                     LRKVGEDGVRFQSPVNGDRLLLTPEESMRIQAALNADVAMIFDECTPYLIDDRPATER
                     EAAASMRLSLRWARRSRDEFDRLGSPNALFGIVQGGMFEALRDESAAGLIDIGFDGYA
                     IGGLSVGEPKEEMMRVLAHLAPRLPADRPRYLMGVGTPEDLLAGVAAGIDLFDCVLPT
                     RNARNGHLFTRWGDVRIRNSRYKDDTRPLDSTCGCYTCRNFTRAYLHHLDRINEILGA
                     RLATIHNLHYYLDLMRQARAALDAARFAAFAREALGARARGPD"
     gene            1760..2104
                     /gene="yajC"
                     /locus_tag="AC16HB_10175"
     CDS             1760..2104
                     /gene="yajC"
                     /locus_tag="AC16HB_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR5607197.1"
                     /translation="MLLISDAFAQSAPAATGGDPGYMSILFMVLIFVVFYFLLIRPQV
                     KRQKEHKAMVDALNKGDEVVTSGGIAGKITEMSDTYVTLQVASVDGKPVALSIQRAAI
                     QMLLPKGTLKAL"
     gene            2195..>2318
                     /locus_tag="AC16HB_10180"
     CDS             2195..>2318
                     /locus_tag="AC16HB_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607198.1"
                     /translation="MNRYPLWKYLVIAVALLIGATYTTPNFFGEAPAVQVSSQKV"
BASE COUNT          366 a          788 c          783 g          381 t
ORIGIN      
        1 tcccgacgac gccgccgacg aggcgcgcta tcagaccgtg tacgcgaaga cgcccggcgc
       61 ggttgccgcg ccgaccgcgg ggctgcactt caccgaggaa ctgctggctc ggctgcagca
      121 caggcgcgtt cgcttcgcgt acgtgacgct gcacgtgggc gccgggacct tccagccggt
      181 gcgcgtcgag ccgatcgccg accacgtgat gcacagcgag tggtatgcga tcgacgcgga
      241 cgcagcgcgc atcgtcaacg acgcgcgcgc ggcgggacgg cgcgtgaccg cggtcggcac
      301 gaccagcctg cgggcgctcg aatcggcggc gcgcgacggc ttggtggaag ccggcgcgcg
      361 cgacacgcgg ctcttcatca cgccgggatt ccgcttccag atcgtcgacc gactggtgac
      421 gaacttccac ctgccgcagt cgacgctgct gatgctggtg tcggcgttcg cggggatgga
      481 gcgcatccgc ggcgcctacg cgcacgcgat cgcgcaacgc tatcgattct tttcctatgg
      541 agatgcgatg ctgctggagc gggccccatg acggcgcggt tcgaactgct cgccaccgag
      601 ggcgaggcgc ggcgcgggcg gctggcgctc aaccacggcg tcgtggagac gccggtcttc
      661 atgccggtgg gcacctacgg cacggtcaag gcgatgacgc cgcgcgaact cgacgaaagc
      721 ggcgcgcaga tcgtgctcgg caacacgttc catctgtggc tgcggccggg cgtggaggtg
      781 atcggcaggc atgccggatt gcaccgcttc atgggctggg acaagccgat cctgaccgat
      841 tccggtggct tccaggtgtt ctccttgggc gcgctgcgca aggtcggcga ggacggcgtg
      901 cgcttccagt cgccggtgaa cggcgaccgc ctgctgctga cgcccgagga atcgatgcgc
      961 atccaggctg cgctgaacgc cgacgtcgcg atgatcttcg acgagtgcac gccgtacctg
     1021 atcgacgatc ggcccgcgac cgagcgcgag gcagcggctt cgatgcggct gtcgctgcgc
     1081 tgggcgcgtc gctcacgcga cgagttcgat cgcctcggca gtcccaacgc gctgttcggc
     1141 atcgtgcagg gcgggatgtt cgaagcgttg cgcgacgaat ccgccgccgg actgatcgac
     1201 atcggcttcg acggctacgc gatcggcggt ctgtcggtcg gcgagcccaa ggaagagatg
     1261 atgcgcgtgc tcgcgcacct cgcgccgcgc ctgcctgccg atcgcccgcg ctatctgatg
     1321 ggcgtcggca cacccgagga cctgctggcc ggcgttgccg ccggcatcga cctgttcgac
     1381 tgcgtgctgc cgacgcgcaa cgcgcgcaac gggcacctgt tcacgcgctg gggcgacgtg
     1441 cggatccgca acagccgcta caaggacgac acgcggccgc tcgactcgac ctgcggctgc
     1501 tacacctgcc gcaacttcac gcgagcgtat ctgcaccacc tcgaccgcat aaacgagatc
     1561 ctcggcgcgc ggctggccac gatccacaac ctgcattact acctcgacct gatgcggcag
     1621 gcgcgcgccg cgctcgacgc cgcccgcttc gcggcattcg cgcgcgaggc actcggcgcg
     1681 cgcgcgcgcg gaccggattg atgcggcggc tcgcaaagcg cgcgctgtct ataatccgcg
     1741 gctttccccg gagttctaca tgctcttgat ttccgacgcg ttcgcgcaga gcgccccggc
     1801 ggccaccggc ggcgatcccg gctacatgtc gatcctgttc atggtgctga tcttcgtcgt
     1861 cttctatttc ctgctgatcc gcccgcaggt gaagcggcag aaggagcaca aggcgatggt
     1921 cgacgcgctg aacaagggcg acgaggtcgt cacctccggc ggcattgccg gcaagatcac
     1981 cgagatgagc gacacctacg tcacgctgca ggtcgccagc gtcgacggca agccggttgc
     2041 gctgtcgatc cagcgcgccg cgatacagat gctgctgccg aaaggcacct tgaaagcctt
     2101 gtgaagcgcg aaatgtgaaa tgtgaatcgt cgtcgcaagc cgcgcactgc cggatcgttc
     2161 accgttcacg tttcacgcct cacgtttcgc cgccatgaat cgctaccctc tctggaagta
     2221 cctcgtcatc gcggtcgcgc tgctgatcgg tgcgacctac acgacgccga acttcttcgg
     2281 cgaggcgccg gccgtgcagg tgtcgtcgca gaaggtga
//
LOCUS       DBNHBY010000409         1763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_498221, whole genome shotgun sequence.
ACCESSION   DBNHBY010000409 DBNHBY010000000
VERSION     DBNHBY010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_498221"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC16HB_10185"
     CDS             complement(<1..1176)
                     /locus_tag="AC16HB_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5607199.1"
                     /translation="MKTMTTMDAPAWSAVPATTAHHYGLTVHAGERQRLAAAWLLLAV
                     GALLASGVFSVLLVAARTPALGAMLPGVDFFRVALVVHVDLSVLVWFAACAGLLWSIA
                     GTERWLGTAWAGWGLATLGSVALALAPFVENALPVMANYVPVLDGSVFIAGLVIVGVG
                     FGLTALRALVVPARVGTALAPEGALRFGLNTAAVAAALALLALAWSAFAVPRELPAKA
                     YYEMLFWGPGHVAQFCWSLIMLVAWLWLAALTGARVPLSPRVMVLLFAIGLASVFATP
                     LIYLAWDVTSVEFQRLFTWQMRFGGGLAIVPVTAAIAIALYASRQRALEAGLKPLRAA
                     LIASVLLFAAGGLIGFLIHGSDVRIPAHYHGSIVGVTIALMGVVYWLLPRLGFAATLP
                     "
     gene            complement(1173..1682)
                     /locus_tag="AC16HB_10190"
     CDS             complement(1173..1682)
                     /locus_tag="AC16HB_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607200.1"
                     /translation="MPWPSPIRYGAQALLYGAFAAFVGYFSTRPTFSLLEPNEGLLRL
                     SFMAPGKPRFECRTRTPEELAKLPPQMRAQTECPRERSPVRVRVELDGVAVIDTAFPP
                     AGLAKDGAAAGYRRLPVPAGTHELKVQVNDDARASGYTHEKRERVAIAPGQIVLIDFN
                     PERGGVLIR"
BASE COUNT          311 a          648 c          598 g          206 t
ORIGIN      
        1 cggcagcgtc gcagcgaagc cgagccgcgg cagcagccag tagacgacgc ccatcagcgc
       61 gatcgtcacg ccgacgatcg agccgtgata gtgcgcggga atgcgcacgt cggatccgtg
      121 gatcaggaag ccgatcagac cgcccgcggc aaacaacagc accgaggcga tcagcgccgc
      181 gcgcagcggc ttcagaccgg cctcgagcgc gcgctggcgc gaggcgtaaa gcgcgatggc
      241 gatcgcggcg gtgacgggca cgatcgccag tccgccgccg aagcgcatct gccaggtgaa
      301 caggcgctgg aactcgaccg atgtcacgtc ccacgcgagg tagatcagcg gcgtggcaaa
      361 taccgatgcc aacccgatcg cgaacagaag caccatcacg cgcgggctga gcggcacgcg
      421 cgcgccggtc agcgcagcca gccacagcca agcgaccagc atgatcaggc tccagcagaa
      481 ctgcgcgacg tgcccggggc cccagaagag catctcgtag tacgccttgg cgggcagttc
      541 gcgcggcacc gcgaacgccg accacgcgag cgcgagcagt gccagcgcgg ccgcgacggc
      601 cgcggtattc agcccgaagc gcagcgcgcc ttcgggcgcc agcgcggtgc cgaccctggc
      661 cggcacgacg agcgcgcgca gcgccgtgag cccgaagccc acgccgacga tcacgagtcc
      721 ggcgatgaac acgctgccgt cgagcaccgg cacgtaattg gccatcaccg gtagcgcgtt
      781 ctcgacgaac ggcgcgagcg caagcgcgac gctgcccagc gtcgccagac cccagccggc
      841 ccacgccgtg ccgagccagc gctcggtgcc ggcgatgctc cataacagcc cagcgcacgc
      901 ggcgaaccac accagtaccg acaggtccac gtgcaccacc agcgcgacgc ggaagaagtc
      961 gaccccgggc agcattgcgc cgagcgcagg cgtgcgcgcc gccaccagca gcaccgagaa
     1021 cacgcccgag gcgagcagcg ctccgactgc gagcagcagc caggccgctg ccaaccgttg
     1081 ccgctccccc gcatgcacgg tcaggccgta gtgatgcgcg gtcgtcgctg gcacggcgga
     1141 ccacgcaggc gcatccatcg tcgtcatggt cttcatcgga tcagcactcc gccgcgttcc
     1201 gggttgaaat cgatcagcac gatctgaccg ggcgcaatcg ccacgcgctc gcgcttctcg
     1261 tgcgtgtagc cgctcgcgcg cgcatcgtcg ttgacctgca ccttcagttc gtgcgtgcct
     1321 gcgggcaccg ggagccgccg gtagccggcc gcggcgccgt ccttcgccag ccccgccggc
     1381 ggaaacgcgg tgtcgatcac cgccacgccg tcgagttcga cgcgcacgcg taccggcgag
     1441 cgctcacgcg ggcactcggt ctgcgcgcgc atctgcggcg gcagcttggc cagttcctcc
     1501 ggtgtgcggg tccggcactc gaagcgcggc ttgcccggtg ccatgaacga cagccgcagc
     1561 agcccctcgt tgggttcgag cagcgagaag gtcgggcggg tcgagaagta gccaacgaac
     1621 gcggcgaacg cgccgtacag cagcgcctgc gcgccatagc ggatcggact aggccacggc
     1681 atggacggat tccctttgct ggtcgagggc cgcctgcacg cgcgcgtctt cgccgtgcgc
     1741 ggcgtcgatc acctgcaccc gcg
//
LOCUS       DBNHBY010000410         6644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_701174, whole genome shotgun sequence.
ACCESSION   DBNHBY010000410 DBNHBY010000000
VERSION     DBNHBY010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_701174"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16HB_10195"
     CDS             complement(<1..658)
                     /locus_tag="AC16HB_10195"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5607201.1"
                     /translation="MTSAVRTYSFEFFPPKSPEGAEKLRAVRARLAEVQPAFFSVTFG
                     AGGSTREGTWNTVMEIHAAGLPVAPHISCIGTTRADVAQLLAGYRAAGIRRLVALRGD
                     VPSGAAGGAGDFRYANELVEFVRAETGNWFHIEVAAYPEMHPQARSPADDLRNFLGKV
                     RAGANSAITQYFYNADAYFRFCDEVVAAGCTAPIVPGIMPITNFTQLARFSDNCGAEI
                     P"
     gene            complement(655..996)
                     /locus_tag="AC16HB_10200"
     CDS             complement(655..996)
                     /locus_tag="AC16HB_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5607202.1"
                     /translation="MRLLLIWLLNSLALFAVTLVLPGVHIEQWWPYALIAVLVLGLVN
                     ALVRPVLVILTLPITVLTLGLFLLVINALLFWAVGSFLEGFKVDGFWWAMAGALLYSF
                     LSWAFSTVLNK"
     gene            complement(1001..1936)
                     /locus_tag="AC16HB_10205"
     CDS             complement(1001..1936)
                     /locus_tag="AC16HB_10205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5607203.1"
                     /translation="MKILVMGAGGVGGYFGALYARAGHEVTFVARGAHLAALRANGLT
                     IESGVTPLRLASVHATDDPASAGAADVVMFCTKLWDVESAGRQIAPVVAKGGIVIPFQ
                     NGVDSHAILRRGLGPERVAGGVAQIAATIKAPGVIAHTGTMARLQVGAFAPALAGPVQ
                     AFAVAGKAAGVNIDAVDDIERALWQKFAMLAALSGTTCLARQPVGAVRSDPDLRATFA
                     AAVREATAIAQAKGIALGADFVERQLGFLDGLPAEMRSSMLNDLTAGNRLEAPWLSGA
                     VARMAYELNLAAPVSATIYAAMKPYLDGAARAPKG"
     gene            complement(2007..3425)
                     /gene="ahcY"
                     /locus_tag="AC16HB_10210"
     CDS             complement(2007..3425)
                     /gene="ahcY"
                     /locus_tag="AC16HB_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5607204.1"
                     /translation="MSAVLKSTPSCDYVVADLKLADWGRKEIRIAETEMPGLMAIREE
                     FAASQPLRGARITGSLHMTIQTAVLIETLQALGAQVRWASCNIFSTQDHAAAAIAAAG
                     TAVFAVKGETLDDYWDYTHRIFEWPDGGHSNMILDDGGDATLLLHLGTRAEKDPSVIA
                     KPTSEEETALFKAIKAKLAKDPAWYSTRIAKIKGVTEETTTGVKRLYQMAQEGRLAFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVAGYGDVGKGSAQALRA
                     LSAQVWVTEVDPICALQASMEGYRVVTMEYACDKADIFVSATGNFHVITLEHMRKMKN
                     QAIVCNIGHFDNEIDVASLKQYRWENIKPQVDHVIFPDGKRIILLAEGRLVNLGCATG
                     HPSYVMSSSFSNQVIAQIELYTRTDKYPVGVYVLPKHLDEKVARLQLKKLGAQLTELT
                     DEQARYIGVSKAGPYKPDHYRY"
     regulatory      complement(3450..3548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(3750..4607)
                     /locus_tag="AC16HB_10215"
     CDS             complement(3750..4607)
                     /locus_tag="AC16HB_10215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607205.1"
                     /translation="MPPIESCAVAAPVAPPKLQPDLDAIKTRQQATWSSGDFAVIGIT
                     LQIVGETLVEAVDIRAGERVIDVAAGNGNATLAAARRFAQVTSTDYVPALLNKSAARA
                     RADGLAVEYRVADAEALPFADGTFDAALSTFGAMFTPDHRRAASELLRVVRSGGRIGM
                     ANWTPEGFIGSLFKVVAAHVAPPSLLKSPALWGTEPYIVELFGAQADDIRCVRKDFNF
                     RYESAAHFVQMFRDYYGPTHKAFAALDAAGQKALAADITALLERYNVGGSNSLVVPGE
                     YLEVVITKQ"
     gene            4800..5816
                     /locus_tag="AC16HB_10220"
     CDS             4800..5816
                     /locus_tag="AC16HB_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR5607206.1"
                     /translation="MSQDTLSDVLRTVRLRGAVFYYVSYRDRWAAEAPAAREIAAAVM
                     PGAEHLIEYHMVAKGNGWAAVAGLEPVRLAAGDIVMFPQGDAHVMSSAPGLQPRRFGA
                     DWVFETRHVPKPMPISYHDGVEQPGAPMPVDTADTVVVCGFIGCDLRPFNPLIAALPR
                     LLHIPATQKGHWIGRVIEQAAAESRSPGPGSDAMLARISEMAFVDAVRRYVDSLPEPT
                     AGWLAGLRDRYVGRALALLHEKPAEPWSLDELGRQVGLSRSALHERFVQFIGTPPMQY
                     LTHWRMQLASNLLRGTNANVASIALEVGYESEAAFARAFKRAVGMPPAAWRRADGARP
                     SSAS"
     gene            complement(5830..6531)
                     /locus_tag="AC16HB_10225"
     CDS             complement(5830..6531)
                     /locus_tag="AC16HB_10225"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR5607207.1"
                     /translation="MRTLGLIGGMSWESTAVYYRLINQEVRARRGPLRSAELLLYSVD
                     FGPIEQLQREGRWDDASEILVAAAQRLRAGGAEAIVLCTYTMHKVADRIERAVELPLL
                     HIADATGAAAVATRARCVGLLATAYTMEQPFLKERLAQRFGLDVLVPSDGERREVHRI
                     IYEELCAGVIRDASRAVYLQVIDRLRQRGAQAIVLGCTEITLLIGTDDCEVPVLDTTA
                     LHARAAVDFLLSDGA"
BASE COUNT         1031 a         2270 c         2217 g         1126 t
ORIGIN      
        1 gcgggatctc ggcgccgcag ttgtcggaga agcgcgccag ctgggtgaaa ttcgtgatcg
       61 gcatgatgcc cggcacgatc ggcgccgtgc agcctgccgc gacgacctcg tcgcagaagc
      121 ggaagtacgc gtcggcgttg tagaagtact gcgtgatcgc cgagttggcg ccggcgcgca
      181 ccttgcccag gaagttgcgc aggtcgtcgg ctggcgagcg cgcctgcgga tgcatttccg
      241 ggtaggcggc gacctcgatg tgaaaccagt tgccggtctc ggcgcggacg aactcgacca
      301 gttcgttcgc gtaccggaaa tcgcccgcgc cccccgccgc acccgatggc acgtcgccgc
      361 gcagcgcgac cagccgccgg ataccggcgg cgcggtagcc cgcgagcaac tgcgcgacgt
      421 ctgcgcgcgt ggtgccgatg caggagatat gcggcgccac tggcagccct gccgcgtgga
      481 tttccatcac cgtgttccac gtgccttcgc gcgtgctgcc gccggccccg aacgtcacgg
      541 agaagaaagc gggctgcacc tcggccagcc gcgcgcgaac tgcgcgcaac ttctcggcgc
      601 cctcgggcga cttgggcggg aagaattcga agctgtaggt gcgcacggcg ctcgtcactt
      661 gttcagcacc gtggaaaacg cccatgaaag gaagctgtac agcagcgcac cggccatcgc
      721 ccaccagaag ccgtcgacct tgaatccttc gagaaaggac cccaccgccc agaacagcag
      781 cgcattgatc accagcagga acagcccgag agtgagcacc gtgatcggca acgtgaggat
      841 caccaggacc ggccgcacca gcgcgttgac caggccgagc accagcaccg cgatcagcgc
      901 gtacggccac cattgctcga tgtgcacgcc gggcagcacc agcgtgacgg cgaacagcgc
      961 gagcgagttc agcagccaga tcagcaggag tcgcatcgga tcatcccttg ggagcgcgtg
     1021 ccgcgccgtc gaggtacggt ttcattgccg catagatcgt cgcgctcacc ggcgcagcga
     1081 ggttcagttc gtacgccatg cgcgcgaccg cgcccgacag ccacggcgcc tcgagccggt
     1141 tgcctgcggt caggtcattg agcatggacg aacgcatctc cgccggcaat ccgtcgagaa
     1201 acccgagctg gcgctcgacg aaatcggcgc cgagcgcgat gccctttgcc tgcgcgatgg
     1261 cggttgcctc ccggaccgcg gcggcgaagg tcgcgcgcag atccgggtcc gagcgcaccg
     1321 cgccgaccgg ctggcgcgcc aggcacgtgg tgcctgacag cgccgcgagc atcgcgaact
     1381 tctgccacag ggcccgttcg atgtcatcga cggcatcgat gttgacgcct gcggcctttc
     1441 ccgccacggc aaaggcctgc acgggtcctg cgagcgcagg cgcgaacgcg ccgacctgca
     1501 gccgcgccat cgtgccggtg tgcgcgatca cgcccggcgc cttgatcgtg gccgcgatct
     1561 gcgccacgcc gcccgccacg cgttcgggcc cgagcccccg ccgcaggatg gcgtggctgt
     1621 cgacgccgtt ctggaacgga atcacgatgc cgccctttgc caccaccggc gcgatctgcc
     1681 tgccggccga ctcgacgtcc cacagcttgg tgcagaacat caccacgtcc gccgcaccgg
     1741 cggacgcagg gtcgtcggtt gcatgcacgg atgcgaggcg cagcggtgtc acgccgcttt
     1801 cgatcgtcag tccgttcgcg cgcagcgccg ccaggtgcgc cccgcgtgcg acgaacgtca
     1861 cctcgtgccc cgcccgtgca tacagcgcac cgaaatatcc gccgacgccg ccggcgccca
     1921 tcaccaggat cttcatacgt caatctccgt ctgcagtcag cggccgctga actggccgcc
     1981 ccgtgcggac agccgactgc cgacgcctag taccgatagt ggtcgggctt gtaaggtccg
     2041 gccttactga ccccgatgta gcgcgcctgc tcgtcggtca gttccgtcag ctgtgcgccg
     2101 agcttcttca gttgcagccg tgcaaccttc tcgtcgaggt gcttcggcag cacgtacacg
     2161 ccgaccgggt acttgtccgt gcgcgtatag agctcgatct gcgcgatcac ctgattcgag
     2221 aacgaactgg acatcacgta gctcggatgc ccggtcgcgc agccgaggtt caccagccgc
     2281 ccttcggcga gcaggatgat gcgcttgccg tccgggaaga tcacatggtc gacctgcggc
     2341 ttgatgttct cccatctgta ctgcttcagg ctggcgacgt cgatctcgtt gtcgaagtgg
     2401 ccgatgttgc agacgatcgc ctggttcttc atcttcctca tgtgctcgag cgtgatcaca
     2461 tggaagttgc cggtcgcgct gacgaagatg tcggccttgt cgcaggcgta ctccatcgtc
     2521 accacgcgat agccctccat cgacgcctgc agcgcgcaga tcggatcgac ctccgtcacc
     2581 cacacctggg cggaaagcgc gcgcagcgcc tgcgccgagc ccttgcccac gtcgccgtag
     2641 ccggcgacga cggcgacctt acccgccacc atcacgtcgg tcgcgcgctt gatgccgtcg
     2701 accagcgact cgcggcagcc gtacaggttg tcgaatttgc tcttcgtgac cgagtcgttg
     2761 acgttgattg ccggaaacgc gagccggcct tcctgtgcca tctgatacag gcgcttcacg
     2821 ccggtcgtcg tctcctcggt cacgcccttg atctttgcga tccgcgtcga gtaccacgcc
     2881 gggtccttgg cgagcttggc cttgatcgcc ttgaagagcg cggtctcctc ctcgctcgtc
     2941 ggcttggcga tcacggacgg gtccttttcc gcccgggtgc cgaggtgcag caggagcgtg
     3001 gcgtcgccgc cgtcatcgag aatcatgttc gagtggccgc cgtcgggcca ctcgaagatt
     3061 cggtgcgtgt agtcccagta gtcgtcgagc gtctcgccct tgaccgcgaa cacggcggtg
     3121 ccggcggcgg cgattgcggc ggccgcatgg tcctgcgtcg agaagatgtt gcagctcgcc
     3181 cagcgcacct gcgcgcccag cgcctgcagc gtctcgatca ggacggccgt ctggatcgtc
     3241 atgtgcagtg agccggtgat gcgcgcgccg cgcagcggct ggctggcggc gaattcctcg
     3301 cggatcgcca tcagccccgg catctcggtt tccgcgatgc ggatttcctt gcggccccag
     3361 tccgcgagct tcaggtcggc gacgacgtag tcgcaagatg gggtggattt gagtacggcg
     3421 ctcatctcgc gccctccttt cgagaaagaa gtttgcgagc gccgttggaa cttgccacct
     3481 gcaggtggtg cttcgagcct ggcgggtgcg cgccgatggc gcacctcgtc gcaacgctcc
     3541 tcgaagcggc agcggattgt agtcgagcgc gggttacacg cgcatctcag ccgggcggga
     3601 cacccgcgcc ttcatctgcg cgcgggtgcc gccgtgccgg tcgacagtga ctacgtagta
     3661 gtccgacata ttgccggcga cgaacttcgc gcgcagcctg ccagcgccgc ggcggcgatc
     3721 gcgatcgggt acaaccgcat cgtcatccct cactgcttgg tgatcacgac ttccaggtac
     3781 tcgcccggca cgacgagcga attgctcccg ccgacgttgt agcgctcgag cagtgcggtg
     3841 atgtcggccg cgagcgcctt ctggccggct gcgtcaagcg cggcgaacgc cttgtgcgtg
     3901 gggccgtagt agtcgcggaa catctgcacg aagtgcgcgg cagactcgta gcggaagttg
     3961 aagtccttgc gtacgcagcg gatgtcgtcc gcctgtgcgc cgaagagctc gacgatgtac
     4021 ggctcggttc cccatagcgc cggcgacttc agcagcgacg gcggtgcgac gtgcgcggcg
     4081 accaccttga acagtgagcc gatgaatccc tcgggcgtcc agttcgccat gccgatccgt
     4141 ccgccgctgc ggacgacacg cagcaattcc gaggcggcac gccgatggtc gggcgtgaac
     4201 atcgcgccga atgtcgacag cgcggcatcg aaagtgccgt cggcaaacgg cagcgcctcg
     4261 gcgtcggcaa cgcggtattc gaccgcgagc ccatcggcgc gcgcacgtgc cgcgctcttg
     4321 ttcagcagcg cgggcacgta gtcggtcgaa gtcacttgcg caaagcggcg cgcggccgcg
     4381 agcgtggcgt tgccgttacc ggcggccacg tcgatgacgc gctcgccggc gcggatatcg
     4441 accgcctcga ccagcgtttc gccgacgatc tgcagcgtga taccgatcac ggcgaagtcg
     4501 ccgctggacc aggtcgcctg ctgtcgggtc ttgatcgcat cgaggtcggg ttgcagcttg
     4561 ggcggcgcga cgggcgcagc gacggcacag gattcaatgg gtggcatggt gctctccttc
     4621 agttcggacg gggctgtcgt tcgacgtgga agcgacggtg cgcattgttg gcggtggccc
     4681 cgcttgacgc ttcacccggc gtcgccgata tttgcccgcc cgtccgcgtt cgctgcgagt
     4741 ggcgccggca cttgatccgg cgtccgcgcg gcttgctcga tcgtccggcg ggaagtccga
     4801 tgagccagga cacgctgtcc gacgttctgc gtaccgtccg cctgcgcggc gcggtgttct
     4861 attacgtcag ctaccgcgac cgctgggccg ccgaagcgcc ggccgcgcgc gagattgccg
     4921 cggccgtgat gccgggcgcc gagcacctga tcgagtacca catggtggcc aagggcaacg
     4981 gctgggccgc ggtggcaggg ttggagcccg tgcggctcgc cgccggcgac atcgtgatgt
     5041 tcccgcaggg cgacgcgcac gtgatgtcga gtgcgccggg actgcagcca cgtcgcttcg
     5101 gtgccgactg ggtgttcgag acgcgtcatg tgcccaaacc gatgccgatc tcgtaccacg
     5161 atggcgtcga gcagccaggg gcgccgatgc cggtggacac tgcagacacg gtggtcgtgt
     5221 gcggtttcat cggctgcgac ttgcgtccgt tcaatccgct gatcgccgcg ctgccgcgcc
     5281 tgctgcatat cccggccacg cagaagggtc actggatcgg ccgcgtgatc gaacaggcag
     5341 ccgccgagtc gcgctcgccc ggtcccggca gcgatgcgat gctcgcacgc atcagcgaga
     5401 tggcgttcgt cgacgcggtg cgccgctatg tcgattcgct gcccgagccc acggccggct
     5461 ggctcgcggg gttgcgcgat cgatacgtcg gacgcgccct cgcgctgctg cacgagaaac
     5521 cggccgagcc gtggtcgctg gatgaactcg gccggcaggt cggcctctct cgctcggcgc
     5581 tgcacgaacg cttcgtgcag ttcatcggca cgccgccgat gcagtacctc acccactggc
     5641 gcatgcaact ggcatcgaac ctgctgcgcg ggaccaacgc caatgttgcg tcgatcgcgc
     5701 tcgaggtcgg ctacgagtcc gaagccgcgt ttgcgcgcgc gttcaagcgc gcggtgggga
     5761 tgcctccggc cgcgtggcgg agggcggacg gcgcaaggcc gtcatccgct tcctgatcgg
     5821 cccgcgcggt catgcgccat cgctcaggag gaaatcgacc gcggcacgcg cgtgcagcgc
     5881 ggtcgtatcc agcacgggga cctcgcagtc gtccgtcccg atcagcagcg tgatctcggt
     5941 gcagccgaga acgattgcct gcgcaccccg ttggcgcaat cggtcgatca cctgcaggta
     6001 gaccgcgcgc gaggcatcgc ggatcacgcc cgcgcacagt tcctcgtaga tgatccggtg
     6061 cacctcgcgc ctttcgccgt ccgacggcac cagcacgtcg agcccgaagc gctgcgccag
     6121 ccgctccttc aggaacggct gctccatcgt gtacgcggtt gcgagcagtc cgacgcagcg
     6181 cgcccgggtc gcgaccgcgg cggcgccggt cgcatcggcg atgtgcagca acggcagttc
     6241 gaccgcgcgc tcgatgcgat cggcgacctt gtgcatcgtg taggtacaca gcacgatggc
     6301 ctctgcaccg ccggcgcgaa ggcgctgcgc ggccgcgacc aggatctcgg atgcatcgtc
     6361 ccagcggcct tcgcgctgca gttgctcgat cgggccgaag tcgacgctgt acagcaagag
     6421 ttcggcggac cgcaacggcc cgcgacgtgc tcgcacttcc tggttgatca ggcggtagta
     6481 gacggccgtg gactcccagc tcatgccgcc aatcagtccc aacgtgcgca tggtgctcct
     6541 cgcttgagta aggggtcgac gaatcggact cgatcatcta tgcggccaac tcgagctctg
     6601 agtctgccgg cagcttcggc agcagtccgc atcgggcgcg ggct
//
LOCUS       DBNHBY010000411         4869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1851677, whole genome shotgun sequence.
ACCESSION   DBNHBY010000411 DBNHBY010000000
VERSION     DBNHBY010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1851677"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="AC16HB_10230"
     CDS             complement(<1..154)
                     /locus_tag="AC16HB_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5607208.1"
                     /translation="MQAQRGRTALITGSTSGIGLGIAEAFARQGANIVLNGFGDAAEI
                     EGLRAGL"
     gene            complement(310..726)
                     /locus_tag="AC16HB_10235"
     CDS             complement(310..726)
                     /locus_tag="AC16HB_10235"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HIR5607209.1"
                     /translation="MTVIAASAAAAAGPAVNVDRDTVAVGGYDVVAYFAAGTPKPGLP
                     EFTARHDGATYRFASAANLAAFRADPDKYVPPYGGYCAYGTARGYKAPIDPTAWHFVD
                     GKLYLNYNHDVQKQWHADIPGYIRKADKNWPKLRDK"
     gene            910..2982
                     /locus_tag="AC16HB_10240"
     CDS             910..2982
                     /locus_tag="AC16HB_10240"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR5607210.1"
                     /translation="MSAEREKPGKSKKTGRDKATGPAPNFLRNIIAADLARGTYEDRH
                     WAGRPGVYDLHEKAPRDPARIRTRFPPEPNGYLHIGHAKSICLNFGLARDYGGRCHMR
                     FDDTNPVKEDQEYVDAILDTVRWLGFDWEHGTEKNLYHASDYFEDMYRFAEHLIATGY
                     AYVDQQSADAIRATRGTLTHPGSNSPWRDRPIEENLRLFREMRAGKHAEGSMILRAKI
                     DMGSPNINMRDPAIYRIRFAEHHMTGSNWVIYPMYSYAHPIEDALENITHSICTLEFE
                     DQRPFYDWTLERIVPVLRRPQFEAAKNRLAAIQEEGTEACRRFALHCRNFADKLGHSR
                     YERRAEAMFASWSKNPDLALNDADEFLKLLREHAECFTPLLQGALDERKPNPFMLPHQ
                     HEFNRLNLSYVVMSKRKLITLVEEKHVDGWDDPRMPTLVGLRRRGYTPQAIQLFVERV
                     GVSKSLQWIDYSVLEQALRDDLDARAPRATAVLKPLKLIIDNYPPESREDCVVPNHPQ
                     KPDMGTRTIPFARELWIEADDFMETAPADYHRLAPGQAVRLRYAYVIRATSVDKDAAG
                     NVTAVHAEYLPETKSGTPGANTVKTRAAIHWLPVDGSVPAEVRLYDRLFTEAQPDASE
                     RDFRELLTPDSKTVLRSFVEAALAEAEPDEKFQFERNGYFVADRKDHSPERPVFNLAV
                     SLKDSWGR"
     gene            3026..3631
                     /locus_tag="AC16HB_10245"
     CDS             3026..3631
                     /locus_tag="AC16HB_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR5607211.1"
                     /translation="MRFQGRLTTWHDDKGYGFITPNGGGPRVFVHRKDFGSARRPRGD
                     ELVTYELTIDERKRHNARNVQYVALAPSRGSPAAGAIVVPALALIFFAFLLSAAAGGK
                     LPWVVSGWVLAVSVATYLAYRLDKDAAKLGQWRTPEQTLHVLSLVGGWPGALIAQRWL
                     RHKSKKASFLVEFVITVALNLAAVGWLYSSSGWQRLRAFVG"
     gene            complement(3664..4065)
                     /locus_tag="AC16HB_10250"
     CDS             complement(3664..4065)
                     /locus_tag="AC16HB_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607212.1"
                     /translation="MKKLLASLVCALAVPASYAADVGVSVVISQPGVYGRIDIGRFPQ
                     PAVIVPQPVIIAPPAGVVVQPQPVYMWVPPGHRKNWARYCHQYNACGYPVYFVRHDWY
                     DQNVRYADRPRGDDRPGKGHGKGKGHGKGRD"
     gene            4153..4524
                     /locus_tag="AC16HB_10255"
     CDS             4153..4524
                     /locus_tag="AC16HB_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5607213.1"
                     /translation="MIGYVTLGTNDLARAVAFYDALLAELGAKRTWEYDRGIGWGLTE
                     NGISLGIMKPFDGKPASVGNGVMVALAATSKEMVDRMYRKALALGGSDEGAAGPRGGG
                     SYYAGYFRDLDGNKLSVYFEG"
BASE COUNT          867 a         1615 c         1626 g          761 t
ORIGIN      
        1 cgaggccggc gcgcagcccc tcgatctcgg cggcgtcacc gaagccgttg aggacgatgt
       61 tcgcgccctg ccgtgcaaac gcctcggcga tgccgagacc gatgccgctg gtcgaaccgg
      121 tgatgagcgc cgtgcggccg cgttgtgctt gcatctgtga actcctggct gagatttgcg
      181 ggagggcgcg tcgcgtgctg tccggctatc gctggcggat cgccgctgcg cgcctcgatc
      241 gcgcttagtc ctgtccgctg cgaagaactt acatgcgggc catgacgatg cgatgacgcg
      301 acgcgcgcat cacttgtcgc gcagcttcgg ccagttcttg tcggccttgc ggatatagcc
      361 gggaatgtcg gcgtgccact gcttctgcac gtcgtggttg tagttcaggt acagcttgcc
      421 gtcgacgaag tgccacgcgg tcggatcgat cggcgccttg tagccgcgcg ccgtgccgta
      481 cgcgcagtac ccgccgtacg gcggcacgta cttgtccggg tcggcgcgaa atgcggcgag
      541 gttcgcggcc gaggcgaagc ggtacgtggc gccgtcgtgg cgcgcggtga actccggcag
      601 accgggcttg ggcgtaccgg cggcgaaata cgcgaccacg tcatagccgc cgaccgcaac
      661 ggtgtcgcgg tcgacgttca ccgcggggcc agcagcggcc gcagccgaag ctgcgatcac
      721 ggtcatgacc agggcgcgtg caatccaatt aggaccgagc atcgaacact cccatcagag
      781 gcgccgcagg tttcgcggcg caaccggggt tcgtcgaccg ctgccgcggc ttcttactaa
      841 gcgccgcggg gctgcggcat tgcagcacgg ctggctacaa tcccagcaca cctcagggat
      901 gccgcgaaca tgagcgccga aagggaaaag ccgggcaaga gcaagaagac cggtcgcgac
      961 aaggcgacgg gaccggcgcc gaacttcctg cgcaacatca tcgccgccga cctcgcgcgc
     1021 ggcacctacg aggaccgcca ctgggccggc cggcccggcg tgtacgacct ccacgagaag
     1081 gcgccgcgcg atccggcgag gatccgcacg cgctttccgc cggagccgaa cgggtatctg
     1141 cacatcggcc acgcgaagag catttgtctc aacttcggac tggcgcgcga ctacggcggg
     1201 cgctgccaca tgcgtttcga cgacacgaac ccggtcaagg aagaccagga gtacgtcgac
     1261 gcgatcctcg acaccgtgcg ctggctcggc ttcgactggg aacacggcac cgaaaagaac
     1321 ctttaccacg cctcggatta cttcgaggac atgtaccgct ttgccgagca cctgatcgcg
     1381 accggttacg cctacgtcga ccagcagagc gccgacgcga tccgcgccac gcgcggcacg
     1441 ctgacccacc ccggcagcaa ctcgccgtgg cgcgatcggc cgatcgagga gaacctgcgc
     1501 ctgttccgcg agatgcgcgc gggcaagcac gccgaaggca gcatgatcct gcgcgcgaag
     1561 atcgacatgg gctcgcccaa catcaacatg cgcgacccgg cgatctaccg catccgcttc
     1621 gccgagcacc acatgaccgg cagcaactgg gtgatctacc cgatgtactc gtacgcgcac
     1681 ccgatcgagg acgcgctgga gaacatcacg cattcgatct gcacgctcga gttcgaggac
     1741 cagcgtccgt tctacgactg gacgctggag cgcatcgtgc cggtgctgcg gcgaccgcag
     1801 ttcgaggccg cgaagaaccg gctcgccgcg atccaggaag aaggcaccga agcctgtcgc
     1861 cggttcgcgc tgcattgccg gaacttcgcc gacaagctcg gccactcgcg ctatgagcgc
     1921 cgcgcagagg cgatgttcgc gtcgtggtcg aagaacccgg acctggcgct gaacgacgcc
     1981 gacgagtttc tcaagctgtt gcgcgagcac gcggaatgct tcacgccgct gctgcagggc
     2041 gcgctcgacg agcgcaagcc caacccgttc atgttgccgc accagcacga gttcaaccgg
     2101 ctgaacctga gctacgtggt gatgagcaag cgcaagctga tcacgctggt cgaggagaag
     2161 cacgtcgacg gctgggacga cccgcgcatg ccgacgctgg ttgggctgcg ccggcgcggc
     2221 tacacgccgc aggcgatcca gctcttcgtc gagcgcgtgg gcgtcagcaa gagcctgcag
     2281 tggatcgact acagcgtgct ggagcaggcg ctgcgcgacg acctcgacgc gcgcgcgccg
     2341 cgcgccaccg cggtgctcaa gcccctgaag ctcatcatcg acaactaccc gccggaaagc
     2401 cgcgaagact gcgtggtgcc caaccacccg cagaagcccg acatgggcac gcgcacgatc
     2461 ccgttcgcgc gcgaattgtg gatcgaggcc gacgacttca tggagaccgc gccggccgac
     2521 tatcaccggc tggcgcccgg tcaggcagtg cggctgcgct acgcgtacgt gatccgcgcg
     2581 acctccgtcg acaaggacgc tgccggcaac gtcaccgccg tgcacgccga gtacctgccg
     2641 gaaacgaaga gcggcacgcc cggcgccaac accgttaaga cccgcgctgc gatccactgg
     2701 ctgccggtcg acggaagcgt gcccgccgag gtgcggctgt acgaccgcct gttcaccgaa
     2761 gcgcagcccg atgcgagcga gcgcgacttc cgcgaactgc tgacgccaga cagcaagacc
     2821 gtgctgcgct catttgtaga agcagcactt gccgaggcag agcccgacga aaagtttcag
     2881 ttcgagcgga acgggtactt cgtcgcggat cggaaggatc actcgccgga gaggccggtg
     2941 ttcaatctgg cggtgtcgtt gaaggactcg tggggtcggt agaagtacgt gtcgcgcaac
     3001 gtagctgacg acgcaggctc aacgagtgcg atttcaaggc cgcctcacca cgtggcacga
     3061 cgacaagggc tacggcttca tcactccgaa cggcggcgga ccgcgcgtgt tcgttcaccg
     3121 taaggacttc ggctccgcgc gccgaccgcg cggcgatgaa ctcgtgacgt atgagttgac
     3181 gatagacgaa aggaagcgtc acaacgcgcg caatgtccaa tacgttgcgc tcgcgccttc
     3241 gcgaggatcg cccgcggccg gtgcgatcgt tgtgcccgca ctggcgctga tcttcttcgc
     3301 ttttcttctg agcgctgcgg cgggcggcaa gctgccgtgg gtcgtgtccg gctgggtcct
     3361 ggccgtgagt gtggcgacct acctcgcgta tcgattggac aaggacgcag cgaaactcgg
     3421 ccaatggcgc acgcccgagc agacgttgca cgtgctttca ctcgtcgggg gctggccggg
     3481 cgcgctgatt gcccagcgct ggctgcgcca caaatcgaag aaggcgtcgt ttctcgtcga
     3541 gttcgtgatc acagtcgcgc tcaatcttgc cgcggtcggg tggttgtact cgagcagtgg
     3601 gtggcaaaga ctgcgcgcct tcgtcggtta ggccggatcc gaccgcaagc tccgcgtcac
     3661 aactcagtcc cgccccttcc cgtgcccctt gcccttgcca tgccccttgc cggggcggtc
     3721 gtcgccgcgc ggccggtcgg catagcggac gttctggtcg taccaatcgt gccggacgaa
     3781 atacaccgga tagccgcacg cgttgtactg gtggcagtag cgggcccagt tcttccggtg
     3841 tccgggcggc acccacatgt agaccggctg cggctgcacg acgacaccgg ccggcggcgc
     3901 gatgatcacc ggctgcggca cgatcaccgc cggctgcgga aagcggccga tgtcgatgcg
     3961 accatacacg cccggctggc tgatcacgac ggacacgccg acgtcggcgg cataggacgc
     4021 cggcacggca agcgcgcaca ccagcgacgc aagaagtttc ttcatgatcg agcctcgtcg
     4081 cgacaacgga tggccaatta tcgcgccgct cgcacctaca ctgcgcatca cacgtggaca
     4141 ggagattcgg ccatgatcgg ttacgtcacc cttggcacca acgacctggc gcgcgccgtg
     4201 gcgttctacg acgcgctgct ggcggaactc ggcgcgaaac gcacctggga atacgaccgc
     4261 ggcatcggct ggggcctgac ggagaacgga atcagcctcg gcatcatgaa gcctttcgat
     4321 ggcaagccgg ccagcgtcgg caacggcgtg atggtggcgc tggcagcgac ctcgaaggag
     4381 atggtcgatc gcatgtaccg caaggcgctc gcgctcggcg gcagcgacga gggagcggcc
     4441 gggccgcgtg gcggcggcag ctactacgcg ggctacttcc gcgacctcga cggcaacaag
     4501 ctgagcgttt atttcgaggg ctgagcatcc gcgagcgcga cgcggcgcgc tcgggctcgc
     4561 gccggcgcgg catgacgtcg ccctcgcggc gtgcgtgctg cgcgggctgg gcatgcggtt
     4621 cgatgccgcg ctggatatca cccggcggcg cttgcctgac ttatcggtgg cagcggcgtc
     4681 gcctgcattg tgcgccgtac gcggcaagca gaagtgctga tcggcgccgc ggagcacgag
     4741 accagcgtga ttcgcccgcg cggcgctagc gcaacgcacg cagcgatgca attccgctcg
     4801 tcggtcgcgc gcgcccgcct ggaaagatcg caatttcgaa gtgggcatgc acgttgtcgg
     4861 acgaattta
//
LOCUS       DBNHBY010000412         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_565821, whole genome shotgun sequence.
ACCESSION   DBNHBY010000412 DBNHBY010000000
VERSION     DBNHBY010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_565821"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..563
                     /locus_tag="AC16HB_10260"
     CDS             90..563
                     /locus_tag="AC16HB_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5607214.1"
                     /translation="MTRRITRDELRAAVGNEVGASGWYPVTQASIDEFADATHDPQWI
                     HVDPERAARESPFRDQSGVGRTVAHGFLTLSLLSHLLQSALEFADRRAGVNIGFNRVR
                     WVAPVLSGMRVRARFVLAACDAIAGGLQLTWDVTVEREGHDKPALVAQWLTRVLY"
     gene            652..2286
                     /locus_tag="AC16HB_10265"
     CDS             652..2286
                     /locus_tag="AC16HB_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607215.1"
                     /translation="MKLASRLAVALAASFLSVSASAHDVAAAGVDPGRAIDPGLGRLH
                     HAVSTRNKQAQAYFDQGMRLLFAFNHEGAAASFARAAQIDPNLAMAHWGIAYALGPNY
                     NLPMSPEAHKTAYAELQKAIALKDRVTPAERDYIDALAKRYSSNPDTDVNPLNVAYKD
                     AMAALARRYPNDVDAQVLYAESLMDLRPWKLWDADGRPAEGTFEIIAVLERALKLDPN
                     HTGANHYYIHALEMSPTPGKALASAHRLETLAPSAGHLVHMPAHTYIRTGDYLKAARA
                     NEIAARADERLRAAGVHSLYTVGYYAHNLHFLAVSYGLAGNAKKSIAAARKLAELLKP
                     QLKDVPFVDGFYATPAQVLVLFDRWDDVLALEEPPFEAPFSGAMHHFARALAFAAKGR
                     AAESKTARTKFNEMVAATGQTAEVGNNSMRDVLAVAASYLDGRLALLANDLPGAVGSL
                     RDAAAAEGKLVYDEPPGWYLGSYWMLGIALLRSGDAAGAEQAFRADLKHNVASGRSLF
                     GLKAALTAQGKASEAAAASEQYERAWRAADVKLATQ"
     gene            2283..2489
                     /locus_tag="AC16HB_10270"
     CDS             2283..2489
                     /locus_tag="AC16HB_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025733.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HIR5607216.1"
                     /translation="MTDFNAGGRLESRCSRCGTPFHCGIDDPGGCWCARLPVLPLGAL
                     EDGRGCLCPDCLAAATLTQPARAT"
     gene            2492..>3630
                     /gene="acpA"
                     /locus_tag="AC16HB_10275"
     CDS             2492..>3630
                     /gene="acpA"
                     /locus_tag="AC16HB_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HIR5607217.1"
                     /translation="MQVARRLAALAVAALGGACAQLPQAGGLERIEHVVVIYAENRSF
                     DHLYGLFLGAEGIAQATMEQKTQLDHDGAPLSALPPVYVNGKPSDAFPQGLPNGPFRI
                     DAPPVNRRLDEKLPSPIHSFYNHREQVNGGRNNLFVAMSDAGAWTMGYFDGSPMKLWQ
                     WAREFTLADHFFTAAFGGSFLNHHWLICACTPAFKDAPLGMRPRLDPDGRLQKRATSP
                     ASVLDGPVQVYNGRVSPDGFVVNNAQPAYQPSGIAPAEGGDLALADPTHSPVPPLHER
                     TLGDALSAKGVGWAWYAEGWNAALADGRRPAREKRKVIYSNVAGSPNFLPHHQPFNYY
                     TRFAPGTADRERHLKDYDDFVAAIDRGALPQVSFYKPAGRYNQHPG"
BASE COUNT          602 a         1240 c         1237 g          551 t
ORIGIN      
        1 gcgaacgagg tgcgcgagca cctgcaggcg atcgccggcg agatggtccg gcgccatgca
       61 cccgatgaag cgcgattgca ggagggcaaa tgacacgccg gatcacgcgt gacgaactgc
      121 gcgccgcggt cggcaacgag gtcggtgcca gcggctggta tccggtgacg caggcgtcga
      181 tcgacgagtt cgccgatgcc acgcacgacc cgcagtggat ccacgtcgat ccagagcgcg
      241 ccgcgcgcga atcgccgttt cgcgatcaga gcggtgtcgg gcgaacggtc gcacacgggt
      301 tcctgacttt gtcgctgctg tcgcacctgc tgcagagcgc gctcgagttc gccgatcgca
      361 gggcgggcgt caacatcggc ttcaatcgcg tgcgctgggt cgcgccggtg ctgtcgggca
      421 tgcgcgtgcg cgcgcgcttc gtgctcgccg cgtgcgacgc gatcgccggc ggcctgcagc
      481 tgacctggga cgtgacggtc gagcgcgagg ggcacgacaa gccggcgctg gtcgcgcagt
      541 ggctgacgcg ggtgctctac tgatgcgccc gcgccgaccg cgatgaccca gaaaccacgg
      601 cgctgcggac atatcccgcc gagttgccgc aacacgttca aggaggttgg catgaagctg
      661 gcatcacgac tggcggtcgc gcttgcggcg tcgttcctgt ccgttagcgc ctccgcgcac
      721 gatgtggcgg cggcgggcgt cgatcccggg cgcgcaatcg atccgggcct gggcaggctg
      781 caccacgcag tctccacgcg caacaagcag gcgcaggcct acttcgatca gggcatgcga
      841 ctgctgttcg cgttcaatca cgaaggcgcg gcggcctcct tcgcgcgcgc ggctcagatc
      901 gatccgaacc tggcgatggc gcactggggg atcgcgtacg cgctgggccc gaattacaac
      961 ctgccgatgt cgccggaggc gcacaaaacc gcttacgcgg agttgcagaa ggcgatcgcc
     1021 ctgaaagacc gggttacgcc ggcggagcgc gactacatcg acgcgctcgc aaaacgctat
     1081 tcgtcgaacc cggacaccga cgtcaacccg ttgaatgtcg cctacaagga cgcgatggcc
     1141 gcgctcgcgc gccgctatcc gaacgatgta gacgcccagg tcctgtatgc ggaaagcctg
     1201 atggacctgc gaccctggaa gctgtgggac gccgacggcc gccccgcaga gggcacgttc
     1261 gagatcattg ccgtgctcga gcgcgcactg aagctcgatc cgaatcacac gggcgcgaac
     1321 cactactaca tccacgcgct cgagatgtcc ccgactccgg gcaaggcgct ggcgtccgcg
     1381 catcgtctcg aaacgcttgc cccgtcggcg gggcacctcg tgcatatgcc ggcgcacacc
     1441 tacatccgca ccggcgacta cctcaaggcg gcgcgcgcga atgaaattgc ggcgcgcgcc
     1501 gacgagcgtc tgcgcgctgc cggcgtgcac tcgctgtaca ccgtcgggta ttacgcgcac
     1561 aacctgcact tcctggcagt cagctacggg ctggcaggca acgcgaagaa gtcgattgct
     1621 gccgcgcgca agctcgccga actgctgaag ccgcaactga aggatgtccc gttcgtcgat
     1681 ggtttctacg cgacgccggc gcaggtcctc gtgctgttcg atcgctggga cgatgtgctc
     1741 gcgctggaag agccgccgtt cgaggcaccg ttcagcggcg cgatgcatca cttcgcccga
     1801 gcgcttgcat ttgccgccaa gggccgcgcg gccgagtcga agacggcgcg cacgaaattc
     1861 aacgagatgg tcgccgccac cggccagacg gccgaagtgg gaaacaacag catgcgcgac
     1921 gtgcttgcgg ttgctgcgtc gtacctggat ggccggctgg ccctgctcgc gaacgacctg
     1981 cccggcgccg tcgggtcgct gcgtgacgcg gctgcggccg aaggcaagct cgtctacgac
     2041 gagccgcccg gctggtatct cggttcgtac tggatgctcg ggatcgcgct gctgaggtcg
     2101 ggtgatgcgg ccggcgccga gcaagccttc cgcgccgacc tgaagcacaa cgtcgccagc
     2161 ggccgctcgc ttttcggact gaaagccgcg ctcaccgcgc agggcaaggc gagcgaagcc
     2221 gccgcggcga gcgagcaata cgagcgcgcc tggcgcgcgg ccgacgtgaa gctcgcaacg
     2281 caatgaccga tttcaacgcc ggcggcaggc tcgagtcgcg ctgcagccgc tgcgggacgc
     2341 cgtttcactg cggcatcgat gatcccggcg gctgctggtg cgcgcggctg ccggtgctgc
     2401 cgctcggcgc gctcgaggac gggcgcggct gcctgtgtcc ggactgcctc gccgccgcaa
     2461 cgctgacgca gccggcgcgc gccacgtgac gatgcaggtc gcgcgccggc tggccgcgct
     2521 cgccgtggcc gcgctcggcg gcgcctgtgc gcagttgccg caggccggcg gcctcgaacg
     2581 catcgagcac gtcgtcgtca tctacgcgga gaaccgcagc ttcgatcacc tgtacggcct
     2641 atttctcgga gccgagggga tcgcgcaggc gacgatggag cagaagacgc agctcgatca
     2701 cgacggcgcg ccgttgtcgg ccctgccgcc ggtgtacgtc aacggcaagc cgagcgatgc
     2761 gtttccgcaa ggccttccca acggcccgtt tcgcatcgat gcaccaccgg tgaatcgcag
     2821 gctcgacgag aagctgccga gtccgatcca cagcttctac aaccatcgcg agcaggtcaa
     2881 cggcggcagg aacaacctgt tcgtcgcgat gtcggatgcg ggcgcctgga cgatgggtta
     2941 tttcgatggc tcgccgatga agctgtggca gtgggcacgc gagttcacgc tggccgacca
     3001 cttcttcacg gccgcattcg gcggctcgtt cctcaaccat cactggctga tctgtgcctg
     3061 cacgcccgca ttcaaggacg cgccgctggg catgcggcca cggctcgacc cggatgggcg
     3121 gctgcagaag cgagcgacgt cgccggcgtc ggtgctggat gggccggtgc aggtctacaa
     3181 cggccgcgtg tcgcccgacg gcttcgtggt caacaacgcc cagcccgcct atcagccgag
     3241 cggcatcgca ccggccgagg gcggcgatct cgcgctcgcg gatccaacgc attcgcccgt
     3301 gccgccgctg cacgaacgca cgctcggcga tgcgctgagc gcaaaaggtg tcggttgggc
     3361 gtggtacgca gagggctgga acgccgcgct cgccgatggt cgccggcccg cgcgcgagaa
     3421 gcgcaaagtc atctattcga acgtcgccgg cagcccgaac ttcctgccgc atcaccagcc
     3481 gttcaactac tacacgcgat tcgcgcccgg caccgcggac cgcgagcggc acttgaagga
     3541 ctacgacgac ttcgtcgccg cgatcgaccg cggcgccttg ccgcaggtga gcttctacaa
     3601 gccggccggc cgctacaacc agcacccggg
//
LOCUS       DBNHBY010000413         1580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1580895, whole genome shotgun sequence.
ACCESSION   DBNHBY010000413 DBNHBY010000000
VERSION     DBNHBY010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1580895"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16HB_10280"
     CDS             complement(<1..959)
                     /locus_tag="AC16HB_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5607218.1"
                     /translation="MPEQQTLFNPASETPDALTLAHFASRAYLDYAISVVKGRALPDV
                     ADGQKPVQRRILYAMNEMGLAANAKPVKSARVVGDVLGKFHPHGDQAAYDALVRMAQD
                     FSLRYPLIDGQGNFGSRDGDGAAAMRYTEARLTPIARLLLDELEEGTTDFAPNYDGTL
                     QEPKLLPARLPFALLNGASGIAVGMATEIPSHNLREVVAALQIVLKNPDAEIDELLAA
                     VPGPDFPGGAQIISPRTDMHEIYANGRGSIKVRARFRFEELARGQGQLVVHELPPGVS
                     ARRVQEEIGELTNPQVKAGKKSLNAEQQQLKQLVLSVLEAQRD"
     gene            complement(1110..1331)
                     /locus_tag="AC16HB_10285"
     CDS             complement(1110..1331)
                     /locus_tag="AC16HB_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HIR5607219.1"
                     /translation="MSKKFPVKPRHPERNCWGCDKYCSAKSMLCGNGSERTQHPVELF
                     GEDWLEWGADAVATGSMLGADTPTLSGAN"
BASE COUNT          248 a          549 c          503 g          280 t
ORIGIN      
        1 tcgtcgcgct gcgcttcgag caccgacagc accagctgct tcaactgctg ctgctcggcg
       61 ttcagcgact tcttgcccgc cttgacctgc gggttcgtga gttcgccgat ctcctcctgc
      121 accctgcgtg cggacacgcc gggcggcagc tcgtgcacca cgagctgccc ctgaccgcgc
      181 gcaagctcct cgaacctgaa gcgcgcgcgc accttgatgc tgccgcgacc gttcgcatag
      241 atctcatgca tgtccgtgcg cggcgagatg atctgcgcgc cgcccgggaa atccggtccc
      301 ggcacagccg cgagcagttc gtcgatttca gcatctggat tcttcagcac gatctgcagc
      361 gcggcgacga cctcgcgcag gttgtgtgac gggatctcgg tcgccatgcc gaccgcgatg
      421 ccgctggcgc cgttgagcag cgcgaacggc aaccgcgcgg gcaggagctt cggctcctgc
      481 agcgtcccgt cgtagttggg cgcgaagtcg gtcgtgcctt cctcgagttc gtcgagcagc
      541 agccgcgcga tcggcgtcag gcgcgcctcg gtgtagcgca tcgctgccgc gccgtcgccg
      601 tcgcgcgaac cgaaattgcc ctgcccgtcg atcagcggat agcgcagcga gaagtcctgc
      661 gccatccgca ccagcgcgtc gtacgcggcc tggtcgccgt gcggatggaa cttgccgagc
      721 acgtcgccga ccacgcgcgc gctcttcacc ggcttggcgt tcgccgccag ccccatctcg
      781 ttcatcgcgt acagaatccg ccgctgtacc ggcttctgcc cgtcggcaac gtccggcagc
      841 gcgcgcccct tgacgaccga gatcgcgtaa tcgagatagg cgcgactcgc gaaatgcgcg
      901 agcgtcagcg cgtcgggggt ttcactcgcc ggattgaaga gtgtttgctg ttcgggcatc
      961 agggttgcgc gagagacgct ggcgcgaggc gggcgagttt accgaaggta gctgcgcgct
     1021 ccgtcgggca ccgcttcatc cctgcgagac gcggcagcac tcgccagcag ccggcgttca
     1081 atgtcgcgcg aaggacgcac gcctcgacgt cagtttgcgc cgctcagagt cggcgtatcc
     1141 gcacccagca tagatcccgt cgcgactgcg tccgcgcccc attccagcca gtcctcaccg
     1201 aacaactcga ccggatgctg cgtgcgctcc gaaccgttgc cacacagcat cgacttcgcg
     1261 gagcaatact tgtcgcaccc ccagcaattg cgctcggggt gtcttggttt cacggggaac
     1321 ttcttggaca tgtcgatacg ccgcggctca cgtctgccgc aggttgctgc gcagtctgcc
     1381 ggcccaggtt ccggcgagat ttgacaggca tcaatcggga tcgccatgac ccgacggggc
     1441 gatggcgttc aagcgcgcag gcaaaaggaa gtcctttcgc cggtgcgact cgtagccgcc
     1501 tgcgcaccga aaccgtggag tggcgactgc attgccaccg cgcagaaacg gcgtagattc
     1561 cgtcgcgcat cgggcgccga
//
LOCUS       DBNHBY010000414         8124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_836641, whole genome shotgun sequence.
ACCESSION   DBNHBY010000414 DBNHBY010000000
VERSION     DBNHBY010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_836641"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16HB_10290"
     CDS             complement(<1..400)
                     /locus_tag="AC16HB_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607220.1"
                     /translation="MPWPSPIRYGAQALLYGAFAAFVGYFSTHPTFSLLEPNEGLLRL
                     SFMAPGKPRFECRTRTPEELAKLPPQMRAQTECPRERSPVRVRVELDGVAVIDTEFPP
                     AGLAKDGAAAGYRRLPVPAGTHELKVQVNDD"
     gene            complement(387..1922)
                     /locus_tag="AC16HB_10295"
     CDS             complement(387..1922)
                     /locus_tag="AC16HB_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR5607221.1"
                     /translation="MHAQLAFAPNAAQLQAPRHAARAIERIERALDAPFGRANPLRQL
                     GALAFLAFWIALGSGAYLYVVFDTSALGAHGSIDALKGEPLLGGLVRGLHRYSADAFA
                     VLTLLHIVREWAHGRYHGFRWFSWLTGVPLLLLLVADGMVGYWLPADTRAQFVATAIG
                     EWVGWLPGFGDGLIRNFVTAEAISDRLFSLLIFLHIGVGLLILLGLWVHLLRLVRPRV
                     NPVRASGFGFAALLVALAVTLPALSTAPADFARAPVNVPIDWFYLAPLALEQGASPGL
                     AWVAVGAIALLLGLLPWTRHEPMAPVVVDPANCTGCARCFDDCPYGAVVMTSRTDTTR
                     FKSIARVDPALCAACGICVGACPRSDALRRVPALVTGIDLPARPLDGMRRRIDSALAH
                     RPGATVVFGCDHGADVAALDRRDVIALSAPCLGQVPPSFADYALRRGACAVLFAGCAD
                     DACAFRFGAGFAHTRLRGTREPHLHPRARGPRVQAIDAARGEEARVQAALDQQRESVH
                     AVA"
     gene            complement(1983..2744)
                     /locus_tag="AC16HB_10300"
     CDS             complement(1983..2744)
                     /locus_tag="AC16HB_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5607222.1"
                     /translation="MSTAHAQERDAAALRVSQSAIGRDAGDWSFTDRQGRSIRLSDYR
                     GKPLVVSFIYTGCFQVCPATTQFLKRATQAARDALGRDSFRVVSIGFNQPFDTPEALA
                     AFARQQGVDLDNWEFLAPRAADVEPLLMRFGMSVNATPAGFDHLIQATVVDASGRIHA
                     QVYGDAFELPLFIGPLKEALAGEAARTLTIENVWRKVKLYCTVYDPASGRYKFNYSLF
                     VEIFAGMTTLGALAWFIGRETRRHLRARRTPSGSA"
     gene            complement(2806..3702)
                     /gene="cyoE"
                     /locus_tag="AC16HB_10305"
     CDS             complement(2806..3702)
                     /gene="cyoE"
                     /locus_tag="AC16HB_10305"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5607223.1"
                     /translation="MTTASIGRGDVMPALRTIFGLFKLRIGLVIAVTALAGAAIQSGP
                     SLTGIQLALLFVTVLMSAGAAGAFNQYYETDLDARMRRTRARPFVSGRLHRGPAWLVA
                     IVALVGIATAASWMALNGAAALYTLAGAFTYAIVYTVWLKRRTWWNIVIGGAAGSFAV
                     LAGAAAVQPELSAAAWLLAVILFLWTPPHFWSLAMVCRDDYAAAGVPMLPVVKGDRVT
                     AYAILGHAVALVLLSLALAWLRPGAFYVACAAIGGAWFVYAALRVAREPSRATAIRCF
                     LASLAQLSLLLLGAMADAVLQF"
     gene            complement(3730..5427)
                     /locus_tag="AC16HB_10310"
     CDS             complement(3730..5427)
                     /locus_tag="AC16HB_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5607224.1"
                     /translation="MASNAAEFRVCPRTGLQFHKSAESLIKANAVAAVVALLVGGILA
                     LLVVLTRWQAVHLIDAENFYMVLTAHGLDMLAFWIIFFEIAILYFCSSTLLRSRLATP
                     KIGWVAFWLMLIGAIMNNVAVFKGDSTVMFTSYVPLPGTPMFYLGLILFAVGALIGCF
                     IFLGTLVVARAEKTYEGSIPLVTFGALTACIIAIFTIASGAIILIPTFLWSVGYIHYI
                     DPLMYRTIWWGFGHSSQQINVSAHVAVWYAIAAIVFGAKPMSERVSRVAFGLYILFLQ
                     LASAHHLLSDPGLGTEWKVFNTSYAMYLAVLASMIHGLTVPGAIEVAQREKGFTKGLF
                     EWIRKAPWGNPVFSGMFISLLGFGFLGGISGVMMGTEQLNMLIHNTIYVPGHFHATVV
                     VGTTLAFMSITYFLIPTLFNREMILPGLAKWQPYLFGLGMSVFCLAMMGAGTLGVPRR
                     HWDITFAGASLPHEFPGTAWLMIAIFAISGLVAIIGGIAYIVITVGSLLFGRKLDAGA
                     YTRQGTVLRMPSVALTGHGSAGFAAPGTFVFALVFLGAFVLYYFVNWKYLSTVWGLS"
     gene            complement(5441..5980)
                     /locus_tag="AC16HB_10315"
     CDS             complement(5441..5980)
                     /locus_tag="AC16HB_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR5607225.1"
                     /translation="MSAIQPPALRVWWKQPIDKVEIIWIGLAFAWCLIMFFMMPYWHV
                     YGKQNLSSETYRVTPALYQKAAEEMVAKYTVRTETDDKIPVVKPPAGSDVYLIARLWA
                     WWPMLELEQGKTYRLHLSAMDYQHGFSLQPENINIQVLPGYEHVVTITPNRAGNYAIV
                     CNEFCGIGHHKMVSRLYVK"
     gene            complement(6000..6170)
                     /locus_tag="AC16HB_10320"
     CDS             complement(6000..6170)
                     /locus_tag="AC16HB_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607226.1"
                     /translation="MEEEKAADKEERIPACQLMLDNPFLLLFLGVAMPTVLYIVWGVM
                     EIVGIPISPLAK"
     gene            6425..>8124
                     /locus_tag="AC16HB_10325"
     CDS             6425..>8124
                     /locus_tag="AC16HB_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR5607227.1"
                     /translation="MIKTIHAAGQRVFLLVERLFSRVFGDAINPLYHLGAISYFMFWV
                     VVASGFYVYAFYETGVDTTFASVERLTHAQWWAGGVMRSLHRYASDAMLLTMLLHFTR
                     HFFFDRYRGFRAFSWLTGIIVLWLVYVAGVNGYMLPWDRLAQYTVVTTSEWFDALPVF
                     RGRMARNFILPEAINDRFFSLLQFVHIGVPLVTLAALWVHTQRVPRARTLPPRGLAIG
                     LIAMLLVLSLARPALSQGAADFLTQPQKLELDWFYLTVYPLVTRGHAEALWLAVAAVT
                     LLFALVPWLPPVRRGAVRAWQLTVHPGNRTVPVRSGETLLDAGLRGGAALPFDCRSGG
                     CGVCRAHVLIGDVDPGVYQPGALSAEQRARGDVLLCCARALTDVEIELEEGAAAREEP
                     LPVYHASVERLERLAPDVMLVALRLDAGRRISYEAGQYANILLEDGARRAYSFTEKGG
                     TTDLVVLHVRRIPNGRFTSHVFDNLRVGDRMRFEGPLGGTVLRDGERPIVFVAGATGF
                     APVKSLLEEAFARGIRAPLALYWGVRKRRDLYMADLPAQWAREHANFRFVPVLSEAGP
                     "
BASE COUNT         1392 a         2775 c         2769 g         1188 t
ORIGIN      
        1 catcgtcgtt gacctgcacc ttcagttcgt gcgtgcctgc gggcaccggg agccgccggt
       61 agccggccgc ggcgccgtcc ttcgccagcc ccgccggcgg aaactcggtg tcgataaccg
      121 ccacgccgtc gagttcgacg cgcacgcgta ccggcgagcg ctcgcgcggg cactcggtct
      181 gcgcgcgcat ctgcggcggc agcttggcca gttcctccgg cgtgcgggtc cggcactcga
      241 agcgcggctt gcccggtgcc atgaacgaca gccgcagcag cccctcgttg ggttcgagca
      301 gcgagaaggt cgggtgggtc gagaagtagc caacgaacgc ggcgaacgca ccgtacagca
      361 gcgcctgcgc gccatagcgg atcggactag gccacggcat ggacggattc ccgttgctgg
      421 tcgagggccg cctgcacgcg cgcctcttcg ccgcgcgcgg cgtcgatcgc ctgcacccgc
      481 ggaccgcgcg cgcgcgggtg caggtgcggt tcgcgcgtgc cgcgcaggcg cgtgtgcgcg
      541 aagccggcgc cgaagcggaa cgcgcaggcg tcgtcggcgc agccggcgaa cagcaccgcg
      601 catgcacctc ggcgcagcgc gtagtcggcg aacgacggtg gtacctgccc caggcacggc
      661 gccgacagcg cgatcacgtc gcgacggtcg agcgcggcca cgtcggcgcc atgatcgcag
      721 ccgaacacga cggtggcgcc gggccgatgc gcgagcgcgc tgtcgattcg gcgccgcatt
      781 ccgtcgagcg ggcgtgccgg caggtcgata ccggtcacca gtgccggcac gcggcgcagc
      841 gcatcggagc gcgggcaggc gcccacgcag atgccgcacg ccgcacacag cgccgggtcg
      901 acgcgcgcga tcgacttgaa gcgcgtcgtg tcggtgcgcg acgtcatcac gacggcgccg
      961 tacgggcagt cgtcgaagca gcgcgcgcag ccggtacagt tggccggatc gacgacgacc
     1021 ggcgccatcg gctcgtgccg cgtccatggc agcagcccga gcagcagcgc gatcgcgccg
     1081 accgcgaccc acgccaaccc cggcgacgcg ccctgctcga gcgcgagcgg cgcgagatag
     1141 aaccagtcga tgggcacgtt gaccggcgcg cgcgcaaagt cggccggcgc cgtcgacagc
     1201 gcgggcagcg tgaccgcgag cgcgacgagc agcgcagcga agccgaatcc gctcgctcgc
     1261 acgggattca cgcgcggccg caccagccgc agcagatgca cccacaggcc aagcaggatc
     1321 agcaagccga cgccgatgtg caggaagatc agcagcgaga acaggcggtc cgagatcgcc
     1381 tcggccgtga cgaagttccg gatcagtccg tcgccgaaac ccggcagcca gccgacccat
     1441 tcgccgatcg cggtggcgac gaactgcgca cgcgtgtcgg ccggcagcca gtagccgacc
     1501 atgccgtcgg cgacgagcag cagcagcagc ggcacgccgg tcagccacga gaaccagcgg
     1561 aagccgtgat agcgaccgtg cgcccactcg cgcacgatgt gcagcagcgt cagaaccgcg
     1621 aacgcgtcgg cgctgtagcg atgcaagccg cgcacgaggc cgccgagcag aggctcaccc
     1681 ttcaaggcat cgatcgaacc atgcgcgccc agcgccgaag tgtcgaacac cacatacagg
     1741 tacgcgccgc tgccgagcgc gatccagaac gcgaggaacg ccaatgcgcc gagttgccgc
     1801 agcggattcg cgcggccgaa aggcgcatcg agcgcgcgtt cgatacgctc gatcgcgcgc
     1861 gcggcgtgcc gtggcgcttg cagttgcgct gcgttcggag cgaaggcgag ttgcgcgtgc
     1921 atggtcgtcg gaatcccgct gcgatcatgc gttgcgcgcg ctgccgcgcg ccttgaccgc
     1981 catcaagcgg aaccagaggg ggtacgccgt gcgcgcaggt gccgccgcgt ttcgcggccg
     2041 atgaaccatg ccagtgcgcc gagtgtggtc atgcccgcga agatctcgac gaacaacgag
     2101 tagttgaact tgtaccgtcc gctcgccggg tcatagacgg tgcagtacag cttgaccttg
     2161 cgccacacgt tttcgatcgt gagcgtgcgc gcggcctcgc cggcgagcgc ctccttcagc
     2221 gggccgatga acagcggcag ctcgaacgcg tcgccataga cctgcgcgtg gatgcggccg
     2281 ctggcgtcga cgacggtggc ctggatcagg tgatcgaagc cggccggcgt ggcgttgacc
     2341 gacatgccga agcgcatgag cagcggttcg acgtcggcgg cgcgcggcgc gaggaactcc
     2401 cagttgtcca ggtccacacc ctgctggcgc gcgaatgcgg cgagcgcctc gggagtgtcg
     2461 aagggctggt tgaagccgat cgataccacg cggaaactgt cgcgtccgag cgcgtcgcgc
     2521 gcggcctgcg tcgcgcgctt gaggaactgc gtggtagccg gacagacctg gaagcagccg
     2581 gtgtagatga agctgaccac caggggcttg ccgcgatagt cggacagccg gatgctgcgg
     2641 ccctggcggt cggtgaagga ccagtcgccg gcatcgcgcc cgatcgcgct ttggctcacg
     2701 cgcagcgcgg cagcatcgcg ctcctgcgca tgcgcggtcg acacagccaa agcaacgacg
     2761 attgcgagct gggtgagcgc gtgccgcagc agcgagcgcg cgcaatcaga actgcagcac
     2821 ggcatcggcc atcgctccca gcaacaacag cgacagctgc gccagcgagg ccaggaagca
     2881 gcggatcgca gtcgcgcgcg acggctcgcg ggcgacgcgc agcgccgcgt agacgaacca
     2941 cgcgccgccg atcgcggcgc aggccacgta gaaggccccc ggccgcagcc acgccagcgc
     3001 cagcgacagc agcacgagcg cgaccgcatg gccgaggatg gcataggccg tcacgcggtc
     3061 gcccttgacc acgggcagca tcggcacgcc ggcagcggcg tagtcgtcgc ggcacaccat
     3121 cgcgaggctc cagaagtgcg gcggcgtcca caggaacagg atcaccgcga gcagccaggc
     3181 tgcggcggaa agctccggct gcacggcggc cgcgcccgcg agcaccgcga agctgccggc
     3241 cgcgccgccg atcacgatgt tccaccacgt acgccgcttc aaccacaccg tgtagacgat
     3301 cgcgtacgtg aacgcgcccg ccagcgtgta cagcgccgcg gcgccgttca atgccatcca
     3361 gctggcggca gtggcgatgc cgaccagcgc gacgatcgca acgagccacg ccgggccgcg
     3421 gtgcaagcgt cccgacacga acggccgtgc gcgcgtgcgc cgcatgcgcg cgtcgagatc
     3481 ggtttcgtaa tactggttga acgcgcccgc cgcgcccgcc gacatcagca cggtgacgaa
     3541 cagcagggcg agttgaattc ccgtcaaaga cggaccggac tggatcgcgg cgccggccag
     3601 cgccgtcacc gcgatcacga gcccgatgcg cagcttgaac agtccgaaga tcgtgcgcag
     3661 cgccggcatg acgtcaccgc ggccgatgct tgctgtggtc atctgcgcgc tcccctcaat
     3721 ggaccacgat cagctcagac cccacaccgt cgacagatac ttccagttga cgaagtagta
     3781 caagacgaat gcaccgagga agacgagcgc gaacacgaac gtgcccggcg cggcgaagcc
     3841 agcgctgcca tgccccgtca acgcgaccga cggcatccgc aggacggtgc cttggcgcgt
     3901 ataggcaccg gcgtcgagct tgcggccgaa cagcagcgag cccacggtga tcacgatgta
     3961 ggcgatgccg ccgatgatcg ccaccaggcc cgagatggcg aagatcgcga tcatcagcca
     4021 cgcggtgccg ggaaactcgt gcggcagcga tgcgcccgcg aacgtgatgt cccagtggcg
     4081 gcgcggcacg ccgagcgtgc cggcgcccat catcgccaga cagaacaccg acatgccgag
     4141 gccgaacagg tacggctgcc acttggccag ccccggcagg atcatctcgc ggttgaacag
     4201 cgtcgggatc aggaagtagg tgatcgacat gaacgcgagc gtggtgccga ccacgaccgt
     4261 ggcgtggaag tgccccggca cgtagatcgt gttgtggatc agcatgttca gctgctcggt
     4321 gcccatcatc acgcccgaga tgccgcccag gaagccgaag cccagcagcg agatgaacat
     4381 gccgctgaac accgggttgc cccacggcgc cttgcggatc cactcgaaca ggcccttggt
     4441 gaagcctttc tcgcgctgcg ccacttcgat cgcgcccggc acggtcaggc catggatcat
     4501 cgacgccagc accgccaggt acatcgcgta ggacgtgttg aacaccttcc attcggtgcc
     4561 cagccccgga tcggacagca ggtggtgcgc ggaggcaagc tgcaggaaca ggatgtacag
     4621 gccgaacgcc acgcgcgaaa cgcgctcgct catcggcttg gcaccgaaca cgatcgcggc
     4681 gatcgcgtac cacacggcga cgtgcgcgct gacgttgatc tgctgcgacg agtgaccgaa
     4741 gccccaccag atcgtgcggt acatcagcgg atcgatatag tggatgtacc cgaccgacca
     4801 caggaaggtc gggatcagga tgatcgcgcc cgaagcgatc gtgaagatcg cgatgatgca
     4861 ggccgtgagc gcgccgaacg tcaccagcgg gatcgagccc tcgtaggtct tctccgcgcg
     4921 cgcgaccacc agcgtgccga ggaagatgaa gcagccgatc agcgcgccga ccgcgaacag
     4981 gatcagtccc aggtagaaca tcggcgtgcc cggcagcggc acgtacgacg tgaacatcac
     5041 ggtcgagtcg cccttgaaca cggcgacgtt gttcatgatc gcgccgatca gcatcagcca
     5101 gaacgcgacc cagccgatct tcggcgtcgc cagccgcgat cgcagcagcg tcgacgagca
     5161 gaagtacagg attgcgatct cgaagaagat gatccagaac gccagcatgt ccaggccgtg
     5221 cgcggtcagg accatgtaga agttctcggc atcgatcaga tgcaccgcct gccagcgcgt
     5281 cagcacgacc agcagcgcca ggatgccgcc gaccagcagc gccaccaccg cggcgaccgc
     5341 gttggccttg atcaggctct cggccgactt gtggaactgc agcccggtgc gggggcagac
     5401 ccgaaactcc gcagcattgc ttgccatgtc ctctcccccc ttatttcacg tacagccgcg
     5461 acaccatctt gtggtggccg atgccgcaga actcgttgca gacgatcgcg tagttgccgg
     5521 cgcggttggg cgtgatcgtc accacgtgct cgtagccggg cagcacctgg atgttgatgt
     5581 tctcgggctg cagcgaaaag ccgtgctggt aatccatcgc cgacaggtgc aggcgatagg
     5641 tcttgccctg ctcgagttcg agcatcggcc accaggccca cagccgtgcg atcaggtaca
     5701 catcggagcc cgcgggaggc ttgacgaccg gaatcttgtc gtcggtctcg gtgcgcaccg
     5761 tgtacttggc caccatctcc tcggcggcct tctggtacag cgccggtgtg acgcggtagg
     5821 tctccgacga caggttctgc ttaccgtaca cgtgccagta cggcatcatg aagaacatga
     5881 tcaggcacca ggcgaatgcc agcccgatcc agatgatctc gaccttgtcg atcggctgct
     5941 tccaccagac gcgtagtgca ggcggctgaa ttgcgctcat cgtctccccc tcgtccggac
     6001 tacttggcca acgggctgat cggaatgccg acgatctcca tcacgcccca gacgatgtac
     6061 agcacggtcg gcatcgcaac gccgagaaac agcagcagga acgggttgtc gagcatcagt
     6121 tggcatgcgg ggatgcgctc ttccttgtcg gccgcctttt cctcttccat gtatgacctc
     6181 cgtgatggac atgggctcgc gcccgcgacg gtcgcggggc tccgtggcgg gcgaagttac
     6241 gcagcgcggc gccgaaagaa attgatgcgc cttaagcaat cgggaagtgc gccttggatg
     6301 ctgcgatgcc cacagatctt ctgcgggcat cgccgcgcgg cggcggttcg atgatgcagg
     6361 ttaagtcttg cgcgccgcag gggacgtagc gtcgcgcgct gatccgcttt caatcggcag
     6421 tccggtgatc aagaccattc acgccgccgg ccagcgcgtc ttcctgctgg tcgagcgcct
     6481 gttcagccgc gtcttcggcg acgcgatcaa tccgctgtac cacctcggcg cgatcagcta
     6541 cttcatgttc tgggtcgtgg tcgcgagcgg cttctacgtg tacgcgttct acgagaccgg
     6601 cgtcgacacc accttcgcct cggtcgagcg gctgacgcat gcgcagtggt gggccggcgg
     6661 cgtcatgcgc agcctgcacc gctatgcgtc ggacgcgatg ctactgacga tgctgctgca
     6721 cttcacgcgc cacttcttct tcgaccgcta tcgcggcttc cgggctttct cgtggctgac
     6781 cggcatcatc gtgctgtggc tggtgtacgt ggccggcgtc aacggctaca tgctgccgtg
     6841 ggaccggctg gcgcagtaca cggtcgtgac gacctccgaa tggttcgacg cgctgccggt
     6901 gttccgcggc cgcatggcgc gcaacttcat cctgccggag gcgatcaacg accgcttctt
     6961 ctcgctgctg cagttcgtgc acatcggcgt tccgctcgtc acgctggccg cgctgtgggt
     7021 gcacacgcag cgcgtaccgc gcgcgcgcac gctgccgccg cgcgggctgg cgatcgggct
     7081 gatcgcgatg ctgctggtgc tgtcgctcgc cagaccggcg ctctcccagg gcgcggcgga
     7141 cttcctcacc cagccgcaga agctcgagct cgactggttc tacctgacgg tgtatccgct
     7201 cgtcacgcgc ggccacgccg aggcgctgtg gctcgcggtc gcggcggtca cgctgctgtt
     7261 tgcgctggtc ccctggctgc caccggtgcg gcgcggcgcc gtgcgcgcgt ggcagctcac
     7321 cgtgcatccg ggcaaccgca cggtgccggt gcgctcgggg gagacgctgc tcgacgccgg
     7381 gctgcgcggc ggcgcggcgc tgcccttcga ttgtcgctcg ggcggctgcg gtgtctgccg
     7441 cgcgcacgtg ctgatcgggg acgtcgatcc cggcgtctac cagccgggtg cgctgagtgc
     7501 cgagcagcgc gcgcgcggcg acgtgctgct gtgctgcgcg cgcgcgctga ccgacgtcga
     7561 gatcgaactg gaggagggcg ccgccgcgcg cgaagagccg ctgccggtct atcacgccag
     7621 cgtcgagagg ctcgagcgcc tggcgcccga cgtgatgctg gtcgcgctgc ggctcgacgc
     7681 gggccgacgg atcagctacg aggcaggcca gtacgcgaac atcctcctgg aggacggtgc
     7741 gcggcgtgcc tactcgttca cggagaaggg cggcacgacc gatctcgtcg tgctgcacgt
     7801 gcggcgcatt ccgaacggcc gcttcacttc ccatgtgttc gacaacctgc gcgttggcga
     7861 ccgcatgcgt ttcgaaggcc cgctcggcgg caccgtgctg cgcgacggcg agcggccgat
     7921 cgtgttcgtc gccggcgcca ccggcttcgc gccggtcaag agcctcctgg aagaagcgtt
     7981 cgcgcgcggc attcgcgcgc cgctcgccct gtactggggc gtgcgcaagc ggcgcgacct
     8041 gtacatggcg gacctacccg cgcagtgggc gcgcgagcac gcaaacttcc ggttcgtgcc
     8101 ggtgctgtcc gaggccggcc ccga
//
LOCUS       DBNHBY010000415         5205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1311079, whole genome shotgun sequence.
ACCESSION   DBNHBY010000415 DBNHBY010000000
VERSION     DBNHBY010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1311079"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16HB_10330"
     CDS             <1..719
                     /locus_tag="AC16HB_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5607228.1"
                     /translation="KTKTVGVEAKPEGLLVSFEGEEAPAEPDYYDMVLLSVGRSPNGR
                     KIGAERAGVAVDQRGYIATDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAA
                     GMKSYFDATVIPSVAYTDPEIAWVGLTEDEAKKSGRKLTKGLFPWSASGRAIANNRDE
                     GFTKLLFDDETHRIVGGGIVGTHAGDLIGEIALAIEMGADAVDIGKTIHPHPTLGESL
                     GMAAEVAHGTCTDVPPAKKR"
     gene            834..1541
                     /locus_tag="AC16HB_10335"
     CDS             834..1541
                     /locus_tag="AC16HB_10335"
                     /inference="COORDINATES: protein motif:HMM:NF042805.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HIR5607229.1"
                     /translation="MTPLLDPDEVLKQMTAQAVAQGESLRSTVRDLTLRALQSRELSL
                     SQIKSVVKNVTEGVNIGALQGKLDTDKAFGQALAGMDDALLKVVEASRIALEQVTGGR
                     EFRDSNLKKALNEIERLEDEFLDTVKKAAAGAGAQVKSQWAGVLADKMEVGTATGQQV
                     MSMMEQYGDQVRAAARAQREAGFKAAHALAQNFHALASGVLIGLSEALNAGGRRPSEA
                     PAAKAAPSRPAAKSRKR"
     gene            1661..2326
                     /gene="rpiA"
                     /locus_tag="AC16HB_10340"
     CDS             1661..2326
                     /gene="rpiA"
                     /locus_tag="AC16HB_10340"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR5607230.1"
                     /translation="MTTQEELKRAVAQAALKFVQPQEVLGVGTGSTVDLFIDALAAAK
                     VDVPAAVSSSERSSKRLADYGIRTLDLNDVERLPVYIDGADEIDPGFSMIKGGGAALT
                     REKIVAAVAGMFVCIADASKRVDMLGRFPLPIEVIPMARAYVTRELAKLGGRPKWREG
                     VVTDNGNVIIDVHDLRIADPVALEARLNALVGVVTNGLFAARGADVLLLGTAEGVKEL
                     RRP"
     gene            2356..3036
                     /locus_tag="AC16HB_10345"
     CDS             2356..3036
                     /locus_tag="AC16HB_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HIR5607231.1"
                     /translation="MRRIIRVLALALTAVPLAYLAVALLLGLIPVNRGFIGAPDGIAI
                     YVRTNGVHAELVLPARGAYDWTREFPLLSIVDPRRLPTLAGFDWIAFGWGDRAFYLQT
                     PRWLDLRPGVAINALAGRGPAAMHVEFLQSAYDYRARRVEIDTAQYQRLVDYVRAGFA
                     RDAQGRAVRIDHPGYFATDAFFEGSGHYSPWLTSNEWVRRGLAQAGIRTAAWSPFDVA
                     LFWHLAAR"
     gene            complement(2992..3195)
                     /locus_tag="AC16HB_10350"
     CDS             complement(2992..3195)
                     /locus_tag="AC16HB_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607232.1"
                     /translation="MQGPLGFLLFWVVALTAGVLLVLVATGARFGWLLLAAVPLAFLG
                     FFALRGPRRSTRREVPEERDIER"
     gene            complement(3208..>5205)
                     /locus_tag="AC16HB_10355"
     CDS             complement(3208..>5205)
                     /locus_tag="AC16HB_10355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5607233.1"
                     /translation="LLPLATDTVRFVGQTVAAVAATSHAIAQRAAEAIAVDYEPLDPV
                     VTLDRALASDAPQLWPDAPNNAVALSQWGDEAACNAAFARAAHVTRLSLLNQRLSPVT
                     LEPRAGAAVFDQASQRLTVHASSQNPAGMQKTLAGVLGLAPEMVRVLVGDVGGGFGLK
                     SMTSPEDAVCAYAARKLGRPVRWRASRLDDFQAAAHGRDQRGDAELALDQEGKILGLR
                     VRIDGNIGAYAYGAGAIINLAIGPKVITGVYHVPALDLRARAVLTNTNIVSAYRGAGR
                     PEAIFLIERLMDKAAAELKIDPAALRRRNLIRPSQMPYRTPMGETFDCGDFPHILERA
                     LRESDWAGFEQRRLESAARGRLRGRAVSTFLEWTGAMLEEAVRLVVEADGRVRVFTAL
                     QAMGQGIETSLLQVLAERLQLDAERIEIVQGDSDVAQGFGSMASRSIYIGGSAMLTVS
                     EKTIEAGRELAGQALEAPVQDISYEAGRFRVAGTDLGIDLGELAARQPERRIAIDTVQ
                     KVGGPSWPNSCHVCEVEIDPDTGMVEVARYTTVDDVGRVINPLIVAGQVHGGIAQAIG
                     QALLEQVVYDESGQLLTGSFMDYCIPRADDVPDFDTHTDESQPCTINPLGAKGVGELG
                     TVGATPTVINAVLDALRPLGVTDIAMPATPERVWRAIRAAK"
BASE COUNT          867 a         1755 c         1809 g          774 t
ORIGIN      
        1 tgaagacgaa gacggtgggc gtggaggcga agccggaagg tctgctggtc agcttcgaag
       61 gcgaagaggc gccggccgag cccgactact acgacatggt cctgctgtcc gtcggccgca
      121 gcccgaacgg caggaagatt ggtgccgagc gcgccggcgt ggcggtcgat cagcgcggtt
      181 acatcgccac cgacaagcag atgcgcacca acgtgccgca catcttcgcg atcggcgaca
      241 tcgtcggcca gccgatgctg gcgcacaagg cggtgcacga agggcatgtc gcggccgagg
      301 ccgcggccgg gatgaagtcg tatttcgacg ccacggtgat cccgtcggtc gcgtacaccg
      361 acccggagat cgcctgggtc ggcctcaccg aggacgaagc gaagaagtcc gggcgcaagc
      421 tgaccaaggg cttgttcccg tggagcgcat cgggccgtgc gatcgccaac aatcgcgacg
      481 agggcttcac caaactgctg ttcgacgacg agacgcaccg catcgtcggc ggcggcatcg
      541 tcggcacgca cgccggcgac ctgatcgggg aaatcgcgct ggcgatcgag atgggcgccg
      601 acgcggtcga catcggcaag acgatccatc cgcatccgac gctgggcgag tcgctcggca
      661 tggcggccga ggtcgcgcac ggcacctgca ccgacgtgcc gccggcgaag aagcgctagc
      721 cgtcgcgcgc ccatcgcgtg aatgcggtgg cggctgcgtg gcatcgtcca tgcgcggggc
      781 aggatttgta tcctgcggtc gtaccgtcgc tcacaagact gaaggagaag ccgatgacgc
      841 cgctgctcga ccccgatgaa gtgctgaagc agatgacggc gcaggccgtc gcgcagggcg
      901 aaagcctgcg ctcgaccgtg cgcgacctga cgctgcgcgc actgcagtcg cgcgagctgt
      961 cgctcagcca gatcaagagc gtggtcaaga acgtgaccga gggcgtgaac atcggcgcgc
     1021 tgcagggcaa gctcgacacc gacaaggcgt tcggccaggc gctggccggc atggacgacg
     1081 cgctgctgaa ggtcgtcgag gcaagccgca tcgcgctgga gcaggtcacc ggcggacgcg
     1141 agttccgcga ctcgaacctg aagaaagcgc tgaacgaaat cgaacggctc gaggatgaat
     1201 ttctcgacac cgtgaagaag gctgccgccg gggccggcgc gcaggtcaag tcgcagtggg
     1261 ctggggtgct cgcggacaag atggaggtcg gcaccgcgac cggccagcaa gtgatgtcga
     1321 tgatggaaca gtacggtgat caggtgcgcg ccgctgcgcg cgcacagcgg gaggccggat
     1381 tcaaggccgc gcacgcgctg gcgcagaact tccacgcgct cgccagcggc gtgctgatcg
     1441 gactgtcgga ggcgctgaac gccggcggcc gcaggccgtc cgaagcgcct gctgccaagg
     1501 cggcgccatc gcggccagcc gcaaagtcgc gcaaacgcta gcgacggttc atcgagcgcc
     1561 ggccgcgccg tgccgatcgg cacggtcgag cttgtcggcg gcgcccgcga cccgcggtca
     1621 tgagcgccgg taacatgcgc gccggaacaa tggagcgcgt gtgacaacac aggaagaact
     1681 gaagcgcgcc gtcgcgcagg cggcgctgaa attcgtgcag ccgcaggagg tgctcggcgt
     1741 cggcactggc tcgaccgtgg acctcttcat cgacgcgctc gccgcggcga aggtcgacgt
     1801 gccggcagcg gtatctagct cagagcgcag cagcaagcgg ctggcggact acggcatccg
     1861 gacgctggac ctgaacgacg tcgagcgcct gcctgtctac atcgacggcg ccgacgagat
     1921 cgaccccggc ttcagcatga tcaagggcgg cggcgcggcg ctgacgcgcg agaagatcgt
     1981 cgccgcggtc gccggcatgt tcgtgtgcat tgccgacgcg agcaagcgtg tcgacatgct
     2041 cggccgcttt ccgctgccga tcgaagtgat tccgatggcg cgcgcgtacg tgacgcgcga
     2101 actggcgaaa ctcggcggcc ggccgaaatg gcgcgaaggc gtggtgaccg acaacggcaa
     2161 cgtgatcatc gacgtgcacg acctgcggat tgccgatccg gtcgcgctgg aggcgcgcct
     2221 caacgcgctc gtcggcgtcg tgaccaacgg gctgttcgcc gcgcgcggcg ccgacgtgct
     2281 gctgctcggc accgccgaag gcgtgaagga actgcggcgg ccgtagtacg cgatcagcga
     2341 cggccgcgcg ctgacatgcg tcgaatcatt cgcgtgctcg cactggcgct gaccgcggtg
     2401 ccgctagcgt atctggcggt ggcactcttg ctcggcctga ttccggtcaa tcgcggattc
     2461 atcggcgcgc cggacggcat cgcgatctac gtacgtacca acggcgtcca cgcagaactc
     2521 gtgctgcccg cgcgcggcgc gtacgactgg acgcgcgagt ttccactgct ctcgatcgtc
     2581 gatcccaggc ggctgccgac gcttgccggc ttcgactgga tcgcgttcgg ctggggcgac
     2641 cgtgcgttct acctgcagac gccgcgctgg ctcgatctgc gtcccggcgt cgcgatcaac
     2701 gcgctcgccg gtcgtgggcc ggccgcgatg cacgtcgaat ttctgcaaag cgcgtacgac
     2761 taccgcgcgc ggcgcgtcga aatcgacacc gcgcaatacc agcgcctggt cgattacgtg
     2821 cgcgccggct ttgcacgcga tgcgcagggc agggcagtcc gtatcgacca tccgggctac
     2881 ttcgcaaccg atgctttctt cgaaggcagc ggtcattact cgccgtggct caccagcaac
     2941 gaatgggtgc ggcgcggcct ggcgcaggcg ggcatccgca ccgccgcgtg gtcaccgttc
     3001 gatgtcgcgc tcttctggca cctcgcggcg cgttgagcgc cggggtccgc gcagcgcaaa
     3061 gaagccaaga aacgccagcg gcacggccgc gagcagcagc cagccgaagc gtgccccggt
     3121 cgcgaccagg accagcagca ctcctgcggt gagcgcgacg acccagaaca gcaggaagcc
     3181 gagcggtccc tgcaccagcg cgcgccgcta cttcgcggcg cggatcgcgc gccatacgcg
     3241 ctcgggcgtg gccggcatcg cgatgtcggt aacgccgagc gggcgcagcg catcgagcac
     3301 tgcattgatc accgtcggcg tggcgcccac ggtgcccagt tcgccgacgc ccttggcgcc
     3361 gagcggattg atcgtgcacg gctgcgactc gtcggtgtgc gtgtcgaagt ccggcacgtc
     3421 gtcggcgcgc gggatgcagt aatccatgaa cgaaccggtc aatagctgcc cggactcgtc
     3481 gtacaccacc tgctcgagca acgcctgccc gatcgcctgt gcgatgccgc cgtgcacctg
     3541 cccggcgacg atcagcgggt tgatcacgcg acccacatcg tcgacggtcg tgtagcgcgc
     3601 gacctcgacc atgccggtgt ccggatcgat ctcgacttcg cacacgtggc aactgttcgg
     3661 ccacgacggt ccgccgacct tctgcacggt atcgatcgcg atgcgacgct cgggctggcg
     3721 cgcggcaagt tcaccgaggt cgatgccgag atcggtaccg gccacgcgga agcggcccgc
     3781 ttcgtaggaa atatcctgca ccggcgcttc gagcgcctgg cccgcgagtt cgcggccggc
     3841 ctcgatcgtc ttctcggaga ccgtcaacat cgccgaaccg ccgatataga tcgaacgact
     3901 ggccatgctg ccgaatccct gcgcgacgtc gctgtcgccc tgcacgatct cgatgcgctc
     3961 ggcgtcgagt tgcagccgct cggcgaggac ctgcagcagg ctcgtttcga taccctggcc
     4021 cattgcctgc agcgcggtga agacgcgcac gcggccgtcg gcttcgacaa cgagtcgcac
     4081 ggcttcctcg agcatcgcgc cggtccattc gagaaaggtc gacaccgcac gtccgcgcag
     4141 cctcccgcgg gcggccgatt caagcctgcg ctgctcgaag cctgcccagt cgctttcgcg
     4201 cagcgcgcgc tcgaggatgt gcggaaagtc gccgcagtcg aacgtctcgc ccattggcgt
     4261 gcggtacggc atctgcgacg gccggatcag gttgcgacgg cgcagcgcgg ccggatcgat
     4321 cttcagttcc gcggcggcct tgtccatcag ccgctcgatc aggaagatcg cttcggggcg
     4381 accggcgccg cgatacgcgg acacgatgtt ggtgttcgtc agcactgccc gcgcgcgtag
     4441 gtcgagcgca ggcacgtggt agacgccggt gatgaccttc ggcccgatgg cgaggttgat
     4501 gatcgcgccg gcgccgtacg catacgctcc gatgttgccg tcgatgcgca cgcgcaggcc
     4561 gaggatcttt ccttcctggt cgagcgccag ttccgcatcg ccgcgctggt cccgcccgtg
     4621 cgcggccgcc tggaaatcgt cgagccggga cgcacgccag cgcacggggc gccccagctt
     4681 gcgcgccgcg tacgcgcata cggcgtcctc gggcgacgtc atcgacttca acccgaagcc
     4741 gccgccaacg tcgccgacga ggacacgaac catttccggc gcaaggccca gcacgcctgc
     4801 cagcgtcttc tgcatgccgg ccgggttttg cgagctggcg tgcaccgtca gccgttggct
     4861 ggcctggtcg aacactgccg caccggcgcg cggctcgagc gttaccggcg acagccgctg
     4921 attgagcagc gagagcctgg tgacgtgagc agcgcgcgcg aacgccgcgt tgcaggcggc
     4981 ctcgtcgccc cactgcgaca acgcgactgc gttgttcggc gcgtcgggcc atagctgcgg
     5041 cgcatcggac gcgagcgcac ggtcgagcgt gaccaccggg tcgagtggct cgtagtcgac
     5101 cgcgatcgct tccgccgcgc gctgtgcgat cgcatgcgag gtcgccgcaa cggccgccac
     5161 cgtctggccg acgaagcgga ccgtatcggt cgcgagcgga agaag
//
LOCUS       DBNHBY010000416         1690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_633867, whole genome shotgun sequence.
ACCESSION   DBNHBY010000416 DBNHBY010000000
VERSION     DBNHBY010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_633867"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1121)
                     /locus_tag="AC16HB_10360"
     CDS             complement(6..1121)
                     /locus_tag="AC16HB_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR5607234.1"
                     /translation="MLLLATRKGAWLFHGDASRRAWRADGPHFLGHVVNHVVLDPRDG
                     RTLLAAAKTGHLGPTIFRSTDLGRTWKEAAKPPAFAKAADGSGRAVDHTFWLTPSTAS
                     EANVWYAGTSPQGLFRSEDGGVTWSPFSMINDDPQYREWMGTVQDGTPDGPKLHSIIV
                     DPRDPRHLYFGMSGGGVHESTDGGRTFAPLVKGMQVVEGFDTKVVTFHDPHCVRMCPS
                     NPDRLYQQNHCGIYRIDRPNDEWIRVGRSMPKKVGDIGFPIVVHPRNDAIAWVLPMDG
                     QTVWPRTSPDGKPAVYVTRNAGKTWRRLDDGLPREQAWWTVKRQAMSIDARDPVGLYF
                     GTTGGELWASRDEGAKWNCIARNLPEIYAVEAAELTA"
     gene            complement(1182..1583)
                     /locus_tag="AC16HB_10365"
     CDS             complement(1182..1583)
                     /locus_tag="AC16HB_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5607235.1"
                     /translation="MIIVKATRDTEAGVMPKEDLIAAMASYHEDLARAGVLVDASGLQ
                     PSARGWRIEYSGNRRSVVDGPFAETKELIAGYTLIQVQSREEALEWTRRFPNPAVDGG
                     AGEIEVRQLFELDDFGPSEAVERFRELGVGE"
BASE COUNT          254 a          605 c          536 g          295 t
ORIGIN      
        1 cacattcatg cggtcagttc cgccgcctcg accgcgtaga tctcgggcaa gttgcgcgcg
       61 atgcagttcc acttcgcgcc ttcgtcgcgg ctcgcccaca gctcaccgcc cgtggtgccg
      121 aagtaaagac cgaccgggtc gcgcgcgtcg atcgacatcg cctgacgctt cacggtccac
      181 caagcctgct cgcgcggcag cccgtcgtcg aggcgccgcc acgtcttgcc ggcgttgcgc
      241 gtcacgtaca ccgcgggctt gccgtcggga ctggtgcgcg gccacacggt ctgcccgtcc
      301 atcggcagca cccaggcgat ggcgtcgttg cgcggatgca cgacgatcgg aaagccgatg
      361 tcgccgacct tcttcggcat gctgcggccg acgcggatcc attcgtcatt cggccggtcg
      421 atgcggtaga tgccgcagtg gttctgctga tacaagcgat ccggattgct cggacacatg
      481 cgcacgcagt gcgggtcgtg gaaggtgacg accttggtgt cgaagccttc gacgacctgc
      541 atgcctttca ccagcggtgc gaaggtcctg ccgccgtcgg tcgactcgtg aacaccgcca
      601 cccgacatgc cgaagtacag gtgccgcgga tcgcgcgggt cgacgatgat cgaatgtagc
      661 ttcggcccgt cgggcgtgcc gtcctgcacg gtgcccatcc actcgcggta ctgcgggtcg
      721 tcgttgatca tcgaaaacgg cgaccacgtg actccgccgt cttccgagcg gaacagcccc
      781 tgcggggacg tgcccgcata ccagacgttt gcctcggacg ccgtcgaggg cgtcagccag
      841 aacgtgtgat cgaccgcgcg gccgctgccg tccgcagcct tggcgaacgc gggcggcttc
      901 gcggcctcct tccacgtgcg accgaggtcg gtcgaccgga agatcgtcgg accgaggtgc
      961 cccgttttcg ctgccgccag cagcgtgcga ccgtcgcgtg gatcgagcac cacgtgattg
     1021 acgacgtggc cgaggaaatg cggcccgtcg gcgcgccacg cgcggcgcga cgcatcgcca
     1081 tggaacagcc acgcgccctt gcgcgtagcg agcaacagca tgacccggtt cgccctgccg
     1141 cgagtcgtcg acttcttcgt tgccacgttc gcctcctgtc tctattcgcc cacgccgagt
     1201 tcgcggaagc gctcgaccgc ttcgctcggc ccgaaatcat ccaattcgaa aagctgtcgc
     1261 acctcgatct cgcccgcgcc gccgtcgacc gcggggttcg ggaaccgccg ggtccactcg
     1321 agcgcctcct cgcgcgactg cacctggatg agcgtgtagc cggcgatcag ttccttcgtt
     1381 tccgcaaacg ggccatcgac cacgctgcgc cggtttcccg aatactcgat gcgccagccc
     1441 ctggcgctcg gctgcaggcc ggaggcatcg acgaggacgc cggctctggc cagatcctcg
     1501 tgatagctgg ccatcgcggc gatcaggtcc tccttcggca tcacgccggc ctcggtgtcc
     1561 ctcgtcgcct tcacgatgat catgaatcac accggcggat ctccttcgac aagggttcgc
     1621 gccgggaaga ctacgcccgg cgcgcgggac cgatctcgca tcacgcggtg ccgcccgcgt
     1681 tgcgctgcgc
//
LOCUS       DBNHBY010000417         5270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1851965, whole genome shotgun sequence.
ACCESSION   DBNHBY010000417 DBNHBY010000000
VERSION     DBNHBY010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1851965"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..614
                     /locus_tag="AC16HB_10370"
     CDS             153..614
                     /locus_tag="AC16HB_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607236.1"
                     /translation="MPADLLIDTGHPAGLRMIERGGFGDPASPKRAVLIECGQHWERR
                     ATDVAIDTLLRFLAIAGTVDRLWANTRLRLMPPDRQRVIRVTEAVVARSMDFHFLVPI
                     EGLGIVARAGTPIAADGDHVWRAPYDDTVLVMPSLRHLRPGNTQVRLGRYD"
     gene            803..1885
                     /locus_tag="AC16HB_10375"
     CDS             803..1885
                     /locus_tag="AC16HB_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HIR5607237.1"
                     /translation="MIETFYDAMRRQGITRRSFLKFCSLTAASLGMGPAYAQQLATAL
                     QTKPRLPVIWVEGLECTCCSESFIRTAHPLAKDVVLSTISLDYHMTLSAAAGDQLRAN
                     AHEIMEKYKGQYILAVEGNPPLGADGMYCIYYGRPFVDVLKEYAQDAKAVIAWGSCAS
                     WGCVQAAKPNPTRATPIDKVILDKPIIKVPGCPPIAEVMSAVVTYVAAFDRLPTLDRQ
                     GRPQMFYSQRVHDKCYRRPHFDAGQFVETWDDEGARKGYCLYKVGCKGPTTYNACSTT
                     RWNEGVSFPIGSGHGCIGCSEDGFWDKGSFYERLTNIKEFGVEANADKVGAVTAGVVG
                     AAIVAHAAVSALQRAASKGKSVDAGK"
     gene            1901..3694
                     /locus_tag="AC16HB_10380"
     CDS             1901..3694
                     /locus_tag="AC16HB_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR5607238.1"
                     /translation="MPVIETQGFKLDNSGKRVVVDPINRIEGHLRIEVNLDKDNVIRN
                     AVSTGTMWRGLEVILKGRDPRDAWAFTERICGVCTGVHALASVRSVEDALGIKIPDNA
                     NIIRNLMHSTLYAQDHLVHFYHLHALDWVDVVNALKADPRKTAELAQSISDWPNSTPG
                     YYRDLAGRLKKFVDSGQLGPFRNGYWGHPAYKLPPEANLMAVGHYLEALDFQKEIVKI
                     QTIFGGKNPHPNWLVGGVPCSLNVDGIGAVGAVNMERLNMVAKIIDDTNEFINKVYIP
                     DLLAVASFYKDWGAIGGGISGQNLLAYGEFPDIANDDSNNSLMMPRGAILGGNLKEIH
                     AVDLKDLGQIQEYVTHSWYKYPDESKGLHPFEGVTEPNFVLGPGTKGTKANIEAVDES
                     AKYSWVKSPRYKGQPVEVGPLARYVIAYAMGKPEFKEPVDMVLKKLDVPITALFSTLG
                     RTAARGLECQWAAGKMKHFFNKLMTNLKAGNVATANVDRWEPRTWPAEAKGAGYCEAP
                     RGALGHWIRIKNTKIDNYQCIVPTTWNAGPRDPKNQIGAYEAALMNTKMAVANQPLEI
                     LRTIHSFDPCLACATHVISPEGEELTRVTVR"
     gene            3708..4397
                     /gene="cybH"
                     /locus_tag="AC16HB_10385"
     CDS             3708..4397
                     /gene="cybH"
                     /locus_tag="AC16HB_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HIR5607239.1"
                     /translation="MAAIGSGTSHAVYVWEAPVRLWHWVMVVAMFMLAVTGYFIGSPL
                     PSAGGEASENFLMGYIRFVHFSAGYIFAIFFVLRVYWLFVGNQYAREIFLVPIKMFSP
                     RWWSGLINQGLYYGFMKREGGSFMGHNPLAMAAMFFMYVLGAVFMICTGFALYGEGTG
                     MGSWAFRWFTSWVMPWLGDSQAVHTWHHLCMWYLIVFSIVHMYMVVREDVFSKETVIS
                     TMINGWRVPKP"
     gene            4415..4744
                     /locus_tag="AC16HB_10390"
     CDS             4415..4744
                     /locus_tag="AC16HB_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR5607240.1"
                     /translation="MATKPGQRRRAAPVLAPRVGARPVARRTPVHPGHFLETRFLKPL
                     RITQQGLAKALGISRRRVNELIRGHRGITADTAVRLAIYFGNDAAFWMQLQTDWDLHE
                     AAKRLNI"
     gene            4924..>5270
                     /locus_tag="AC16HB_10395"
     CDS             4924..>5270
                     /locus_tag="AC16HB_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5607241.1"
                     /translation="MKEPSAQVLVLGIGNRLWADEGFGVRALESFNDAYEADGVLLMD
                     GGTQGLNLLEPVVSHRGVLVFDAIDFGLAPGTPKVLRGREVPAWAGTKMSLHQSSFQE
                     LLAIAELQCRFPA"
BASE COUNT          932 a         1690 c         1780 g          868 t
ORIGIN      
        1 attccgtgga actcaggcgc gcgcgcgaac tgcggccgtt cgtcgaccgc gccgactccg
       61 tgctcgacct gcattcgatg cacgaaccct gccgcccaat catggtgtgc gggtaggtcg
      121 acaagaacgc gcagttcgcg cagcagatcg gtgtgccggc ggatctcctc atcgataccg
      181 ggcatccggc cgggctgcgg atgatcgagc gcggcggctt cggcgacccg gccagcccga
      241 agcgcgcagt gctgatcgag tgcggtcagc actgggagcg ccgcgctacc gacgtggcga
      301 tcgatacgct gttgcgcttc ctcgcgatcg caggcaccgt cgaccgcctg tgggcaaata
      361 cacggctgcg gctgatgccg cccgacaggc agcgcgtgat ccgcgtcacc gaggcggtgg
      421 tcgcgcggtc gatggacttt catttcctgg tcccgatcga ggggctcggc atcgtcgcgc
      481 gcgccggcac gccgatcgcc gccgatggcg accacgtctg gcgcgcgccg tacgacgaca
      541 ccgtgctggt gatgccgtcg ctgcgccatc ttcgccccgg caacacgcag gtgcggctcg
      601 gccggtacga ctgaacgaag ccgactgcgg cgctcggaaa actcgtcgtg taacgatgcg
      661 cgttacatcg ctgccgcttt tccgtaccgc ttgacggccg tcaagcgcgc cgatgggcgc
      721 gggcgcacgc tcgctttcga agttcgaaga caaacgcgct gcccgcgcag cgcgtccttc
      781 gtagccacct tcggaggcgt gaatgatcga gacgttctat gacgcgatgc gccggcaagg
      841 cattacgcga cgcagcttcc tcaagttctg cagcctgacc gcggcctcgc tcggcatggg
      901 gcccgcgtat gcgcagcagc ttgccacggc tctgcagacc aagccgcgcc tgcccgtgat
      961 ctgggtcgag gggctcgagt gcacgtgctg cagcgaatcg ttcatccgca ccgcgcaccc
     1021 gctggccaag gatgtcgtgc tgtccacgat ctcgctcgac taccacatga cgctttcggc
     1081 ggcggcgggc gaccagctgc gcgccaatgc gcacgagatc atggagaagt acaagggcca
     1141 gtacatcctc gcggtcgagg gcaatccgcc gctcggcgcg gacggcatgt actgcatcta
     1201 ttacggccgc ccgttcgtgg acgtgctgaa ggaatacgcg caagacgcca aggcggtgat
     1261 cgcctggggc tcgtgcgcat cgtggggctg cgtgcaggcc gccaagccca atccgacccg
     1321 cgccacgccg atcgacaagg tgatcctcga caagccgatc atcaaggtgc cgggttgccc
     1381 gccgatcgcc gaagtgatga gcgcggtcgt gacgtacgtc gccgcgttcg atcgcctgcc
     1441 gacgctcgac cgccaggggc ggccgcagat gttctacagc cagcgggtcc acgacaagtg
     1501 ctatcgccgg ccgcacttcg acgccggcca gttcgtcgag acctgggacg acgagggcgc
     1561 gcgcaagggt tactgcctgt acaaggtcgg gtgcaaggga ccgacgacct acaacgcctg
     1621 ctcgaccacg cgctggaacg agggcgtgag cttcccgatc ggctcggggc atggctgcat
     1681 cggctgctcc gaagacggct tctgggacaa gggctcgttc tacgagcggc tcacgaacat
     1741 caaggagttc ggcgtcgaag ccaacgccga caaggtcggc gccgtgacgg ccggcgtggt
     1801 cggcgcggcc atcgtcgcgc acgcggcggt cagcgcgctg cagcgcgcgg cgtccaaggg
     1861 caagtccgtt gatgcgggca agtagttggg agattgacaa atgccagtga tcgaaaccca
     1921 aggcttcaag ctcgacaaca gcggcaagcg cgtcgtcgtc gaccccatca accgcatcga
     1981 ggggcacctg cgtatcgagg tcaacctcga caaggacaac gtcatccgca acgccgtgtc
     2041 gaccggcacg atgtggcgcg gcctcgaagt gatcctgaag ggtcgcgacc cgcgcgatgc
     2101 gtgggcgttc accgagcgga tctgcggcgt gtgcacgggc gtgcatgcgc tggcgtcggt
     2161 gcgttcggtc gaggacgcgc tcgggatcaa gatccccgac aacgccaaca tcatccgtaa
     2221 cctgatgcat tcgacgctgt acgcgcagga ccacctggtg cacttctatc acctgcacgc
     2281 gctcgactgg gtcgatgtgg tcaacgcgct gaaggcggat ccgaggaaga cggccgagct
     2341 ggcacagtcg atctccgact ggccgaactc cacgcccggc tattaccgcg atctcgcggg
     2401 caggctgaag aagttcgtcg acagcggcca actcggcccg ttccgcaacg gctactgggg
     2461 ccacccggca tacaagctgc cgccggaggc gaacctgatg gcggtcggcc actatctgga
     2521 agcgctcgac ttccagaagg agatcgtcaa gatccagacc atcttcggcg gcaagaaccc
     2581 gcatccgaac tggctggtcg gcggcgtgcc gtgctcgctc aacgtcgacg gcatcggcgc
     2641 ggtcggcgcg gtcaacatgg aacgcctgaa catggtggcg aagatcatcg acgacaccaa
     2701 cgagttcatc aacaaggtct acatcccgga cctgctcgcg gtcgcgtcgt tctacaagga
     2761 ctggggcgcg atcggcggcg gtatctcggg ccagaacctg ctcgcgtacg gcgagtttcc
     2821 ggacatcgcc aacgacgact ccaacaacag cctgatgatg ccgcgcggcg cgattctcgg
     2881 cggcaacctg aaggagatcc acgcggtgga tctcaaggac ctgggccaga tccaggagta
     2941 cgtgacgcac agttggtaca agtacccgga cgagagcaag ggactgcacc cgttcgaggg
     3001 cgtgacggaa ccgaacttcg tgctcgggcc cggaacgaaa gggaccaagg ccaacatcga
     3061 ggcggtcgac gagagcgcca agtactcgtg ggtcaagtcg ccgcgctaca agggccagcc
     3121 ggtggaagtc ggaccgctgg cgcgctacgt cattgcctat gcgatgggca aacccgagtt
     3181 caaggagccc gtcgacatgg tgctgaagaa actcgacgtg ccgatcacgg cgctgttctc
     3241 cacgctcggc cgcacggcgg cgcgcgggct ggagtgccag tgggcggccg gcaagatgaa
     3301 gcacttcttc aacaagctga tgacgaacct gaaggccggt aacgtcgcga ccgccaacgt
     3361 cgaccgctgg gagccgcgca cctggcccgc cgaggccaag ggcgccggat actgcgaggc
     3421 gccgcgcggc gcgctcgggc actggatccg gatcaagaac accaagatcg acaactacca
     3481 gtgcatcgtg ccgaccacgt ggaatgcggg gccgcgcgat ccgaagaacc agatcggcgc
     3541 gtacgaggcg gcgttgatga acacgaagat ggcggtggcg aaccagccgc tcgagatcct
     3601 gcgcacgatt cacagcttcg acccgtgcct cgcgtgcgcc acgcacgtga tctcgcccga
     3661 gggcgaggaa ctcacccgcg tcacggtgcg ctgacaagga ggttcacatg gcagcaatcg
     3721 gaagcggcac ttcccacgcg gtgtatgtgt gggaagcccc ggtgcgcctg tggcactggg
     3781 tgatggtggt cgcgatgttc atgctggcgg tgacgggcta cttcatcggc agcccgctgc
     3841 cgtcggcggg cggcgaggcg tccgagaact tcctgatggg ctacatccgc ttcgtccact
     3901 tctcggcagg ctacatcttc gcgatcttct tcgtattgcg cgtctactgg cttttcgtcg
     3961 gcaatcagta cgcgcgcgag atcttcctcg tgccgatcaa gatgttctcg ccgcgctggt
     4021 ggagcgggct catcaaccag gggctgtact acggcttcat gaagcgcgag ggcggcagtt
     4081 tcatgggcca caacccgctg gcgatggcgg cgatgttctt catgtacgtg ctgggcgcgg
     4141 tgttcatgat ctgcaccggc tttgcgctgt acggcgaagg caccggcatg gggagctggg
     4201 cgttccgctg gttcacgtcg tgggtgatgc cctggctcgg cgacagccag gccgtgcaca
     4261 cctggcatca cctctgcatg tggtacctga tcgtcttctc gatcgtgcac atgtacatgg
     4321 tcgtgcgcga ggacgtgttc tcgaaggaga ccgtgatcag cacgatgatc aacggctggc
     4381 gggtgcccaa gccatgattg cggaggaggg gggaatggcg accaagccgg ggcagcgcag
     4441 gcgcgctgcc ccggtgttgg cgccacgcgt cggagcgcgc ccggtggcgc gccgcacgcc
     4501 ggtgcacccg gggcacttcc tcgagacgcg gtttctgaag ccgctgcgta tcactcagca
     4561 ggggctcgcc aaggcgctcg gcatctcgcg tcgccgcgtc aacgaactga tccgcggcca
     4621 tcgcggcatc accgcggaca ccgcggtgcg gctggcgatc tacttcggca acgacgccgc
     4681 cttctggatg cagctgcaga ccgactggga tctgcacgag gctgcaaagc gcctgaacat
     4741 ctgaacggaa gcacgcttgc cgcgcgatgt cggagcgttg cgcgggggca ttggcgcgcg
     4801 ctcgtgttgc gttgacgccc gtcaacccga tctgccaccg gggacgtaac gtcgcgctca
     4861 ggtgagctgt tacggttcgc gtaacagctt cgatcctcga tcgttcggct ttcgctcccg
     4921 cgcatgaaag agccatcggc acaggtgctg gtcctcggca tcggcaaccg gctgtgggcc
     4981 gacgagggct tcggcgtgcg cgcgctggag tcgttcaacg acgcgtacga agccgacggc
     5041 gtgctgctga tggacggcgg cacgcagggg ttgaacctgc tcgagccggt cgtcagccat
     5101 cgcggcgtgc tggtgttcga cgcgatcgac ttcggactcg cgccgggcac gcccaaggtg
     5161 ctgcgcgggc gcgaggtgcc ggcgtgggcg ggcacgaaga tgagcctgca tcagtcgagc
     5221 ttccaggaac tgctggcgat cgccgagctg cagtgccgct ttcccgcgca
//
LOCUS       DBNHBY010000418         2054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_228695, whole genome shotgun sequence.
ACCESSION   DBNHBY010000418 DBNHBY010000000
VERSION     DBNHBY010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_228695"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="AC16HB_10400"
     CDS             <1..905
                     /locus_tag="AC16HB_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607242.1"
                     /translation="AAVFSLLPVIAKGSTFSSANGYAFLLTAIGIGAVATALALPRLR
                     SRFTYDQMIALSALLFAVALAVPALTHGFPWMLATMPLAGAAWLTTLSSLQSAVQHVA
                     PAWVRARALSIYFMTLNGAIAVGGVLWGTLASSVSVSAALGVAAAALGASAFWFARGR
                     IGTAEASDSGETGEAPAPHFADDVPNDAGPVMVTIEYDVRPDARDAFIAEMRELGRLR
                     RRDGAFAWECSFDIRNRSLAVEAFQVESWLEHLRQHERQGPQVHRLQERVRALCGQPP
                     RARHYVSINAGPLQLEPHPRVADE"
     gene            984..1310
                     /locus_tag="AC16HB_10405"
     CDS             984..1310
                     /locus_tag="AC16HB_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5607243.1"
                     /translation="MRRRSSFAALALALVATAAAAEDEIALTIQDHKFQPAELRVPAG
                     RKVKLVITNRDASAEEFESHALNREKVVPPGKSVAVFIGPLAPGRFPFFGEYHEKTAQ
                     GVVIAE"
     gene            1315..>2054
                     /locus_tag="AC16HB_10410"
     CDS             1315..>2054
                     /locus_tag="AC16HB_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR5607244.1"
                     /translation="MLGAALIVFRETLEAALLVGIIAAATSALPAARRWVGAGVAVGV
                     TGAVVLALAMSRIAEAFDGMGQDVLNAAILAAAVAMLGWHAVWMARHGAQLAADARAL
                     GDSVRRGGAALSAAFLAVALAVLREGAETVLFMFGLLSAGDANPAGVAASGVLGAVGG
                     AALGVLIYAGFRRVPLRTLFSVTGVLIVFFAASLAAQLARQLIQADLLPALIATLWDT
                     SNWLPMDSVFGALLHALIGYEARPSAMQV"
BASE COUNT          274 a          673 c          751 g          356 t
ORIGIN      
        1 ccgcggccgt gttctcgctg ttgccggtga tcgccaaggg ttcgaccttc agcagcgcca
       61 acgggtacgc cttcctgctg accgcgatcg gcatcggtgc ggtggctacc gcgctcgcgc
      121 tgccgcggct gcgcagccgg ttcacctacg accagatgat cgcgttgtcc gcgctgctgt
      181 ttgccgtcgc gctcgcggtg ccggctctga cgcacggatt tccctggatg ctggcgacga
      241 tgccgcttgc cggcgcggcg tggctgacca ccttgtcgag cctgcagagc gccgtgcagc
      301 acgtggcgcc ggcgtgggtg cgggcacggg cgctgtcgat ctacttcatg acgctcaacg
      361 gtgccattgc ggtaggaggt gtgctgtggg gcacgctcgc gtcgagcgtg tcggtgtccg
      421 ccgcgctggg cgtcgcggcg gcggcacttg gcgcgtcggc cttttggttc gcgcgcggcc
      481 gaatcggcac ggcggaggcg agcgacagcg gcgaaaccgg cgaagcgccg gcgccgcact
      541 tcgccgacga cgtgccgaat gacgccggtc ccgtgatggt cacgatcgag tacgacgtgc
      601 gtcccgatgc gcgcgacgcg ttcatcgccg agatgcgcga actcggccgc ctgcgccggc
      661 gggacggcgc gttcgcgtgg gagtgcagct tcgacatccg caaccgcagc ctcgcggtcg
      721 aggcgttcca ggtggaaagc tggctcgagc acctgcgtca gcacgaacgg caggggccgc
      781 aggtccaccg gttgcaggag cgcgtgcgcg cgctgtgcgg gcaaccgccg cgcgcgcggc
      841 actacgtgtc gatcaatgcc gggccgctgc aactggagcc gcacccgcgc gtcgccgacg
      901 agtaaacagg ttgcgccgga ttcagttgac ttgtattctt atttgcgaat gattatcatt
      961 cgatcaagcc ataaggcaaa cccatgcgcc gccgatccag cttcgccgcc ctcgctctcg
     1021 cgctcgttgc cacggccgcc gccgcggaag acgaaatcgc gctcacgatt caggaccaca
     1081 agttccagcc cgccgagcta cgggtgccgg cgggccgtaa ggtcaagctc gtgatcacga
     1141 accgcgacgc cagcgccgag gagttcgaaa gccacgcgct caatcgcgag aaggtggtgc
     1201 cgcccggcaa gtcggtagcg gtgttcatcg gcccgttggc gccgggacgc tttccgttct
     1261 tcggcgaata ccacgagaag accgcgcagg gcgtggtgat cgcggaatag caccatgctc
     1321 ggcgccgcgc tgatcgtatt tcgcgaaacg ctggaagcgg cactgctggt cggcatcatc
     1381 gcggcggcga cgtcggcgct gccggcggcg cgccgctggg tcggcgccgg tgttgcggtt
     1441 ggggtgaccg gcgcggtcgt gctggcgctg gcgatgagtc gcattgccga agcgttcgat
     1501 ggcatggggc aggacgtgct gaacgcggcg atcctcgccg ccgctgtggc gatgctcggc
     1561 tggcacgcgg tgtggatggc gcgtcacgga gcgcagctag cggccgatgc gcgcgcactc
     1621 ggcgacagcg tgcgccgcgg cggcgccgcg ctgtcggcgg cattcctcgc cgtggcgctc
     1681 gcagtgctgc gcgaaggcgc ggagaccgtg ctgttcatgt tcggcttgct ttcggccggc
     1741 gatgcaaatc ccgccggcgt ggcggcaagc ggggtgctcg gcgccgtggg cggtgccgcg
     1801 ctcggcgtgc tgatctatgc cggcttccgc cgggtgccgc tgcgcacgct gttcagcgtg
     1861 accggcgtgc tgatcgtgtt ctttgctgcg tcgctcgccg cgcaactggc gcggcaactg
     1921 atccaggccg acctgctgcc tgcgttgatc gccacgctgt gggatacgtc gaactggctg
     1981 ccgatggact cggtctttgg cgccctgctg cacgcgctga tcggttacga ggcgcggccg
     2041 agcgcgatgc aggt
//
LOCUS       DBNHBY010000419         1761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1243937, whole genome shotgun sequence.
ACCESSION   DBNHBY010000419 DBNHBY010000000
VERSION     DBNHBY010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1243937"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC16HB_10415"
     CDS             <1..921
                     /locus_tag="AC16HB_10415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5607245.1"
                     /translation="RHVDSLELLRSDGTRMRVDPRTQPHWWSATVGGLGLTGLIVAAT
                     LRLKAVPGPWLMQTVRRFRHLDEFFDIDAELRRVHDYTVAWVDCAAPARERGRGLYMA
                     ANFTSDGSAPRRAGAAVRMPFDPPVSLIGALTLRAFNFAYFHLPRPVGTHPVDHQNFF
                     YPLDRVLEWNRIYGPRGFFQFQCALPPACMRDALRDLLDLIARRGAGSFLAVLKTFGD
                     LPREGLLSFGRPGVTLALDFPNRGAATRALFAEMERAVIAAGGALYPAKDALMSPAAF
                     QRSYPRWQDMRAFIDPAFSSEFWRRVAIEP"
     gene            918..1664
                     /locus_tag="AC16HB_10420"
     CDS             918..1664
                     /locus_tag="AC16HB_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607246.1"
                     /translation="MKNIVVFGASSAIAQACARRWCERGDQLLLVARDPARLEEIAAD
                     LRVRGGAVAGVHCFVMDALETARLAELIAFVRARFATVDAVLVAHGTLPDQARCESST
                     ADTIAAIEVNGVSTVAILAAFAQLLEAQKRGTLAVIGSPAGDRGRASNYTYGAAKALV
                     HTFADGLRHRLWRCGVAVVTIKPGFTDTPMTAAKQKGGPLWATPARVARDIVAAIDQG
                     RPVVYTPWFWRWIMLAIVHLPQRLFVRTRL"
BASE COUNT          227 a          604 c          620 g          310 t
ORIGIN      
        1 cgccacgtcg attcgctcga actgctgcgc agcgacggca cgcgcatgcg cgtggatccg
       61 cgcacgcagc cgcactggtg gtcggccacc gtgggcggac tcgggctgac cgggctgatc
      121 gttgccgcca cgctgcggct gaaggccgtg cccgggccct ggctgatgca gaccgtgcgc
      181 cgcttccggc acctggatga gttcttcgac atcgacgccg aattgcgtcg cgtgcacgac
      241 tacacggtgg cctgggtcga ctgcgcggcg cccgcgcgcg agcgcggccg gggcctctac
      301 atggcagcca acttcacctc ggacgggtcg gcgccgcgcc gcgccggagc ggccgtcagg
      361 atgccgttcg acccgcccgt gtcattgatc ggggcgctga cgttgcgcgc gttcaacttc
      421 gcttactttc atcttccgcg ccccgtcggc acgcacccgg tcgaccacca gaatttcttt
      481 tacccgctcg accgggtgct ggagtggaac cgcatctatg gtccgcgcgg cttcttccag
      541 ttccagtgcg cgctgccgcc cgcatgcatg cgcgacgccc tgcgcgatct gctggatctg
      601 atcgcgcgtc gcggtgctgg ctcttttctc gcggtgctga agaccttcgg cgacctgccg
      661 cgggagggcc tgctgtcgtt cggtcgtccc ggcgtgacgc tggccctgga cttcccgaac
      721 cgcggcgcag cgacgcgcgc gctgtttgcg gagatggagc gcgcggtcat cgctgccggc
      781 ggcgcgctct atccggcgaa ggatgcgttg atgtcgcccg cggcatttca acgcagctat
      841 cctcgctggc aggacatgcg cgccttcatc gatcccgctt tctcgtccga attctggcgc
      901 cgcgtggcga ttgaaccgtg aagaacatcg tcgtcttcgg cgccagttcg gcgattgcac
      961 aggcctgcgc gcggcgctgg tgcgagcgcg gcgaccagtt gctgctggtc gcgcgcgatc
     1021 ccgcgcggct cgaagagatc gcagcggatc tgcgcgtgcg cggcggcgcg gtggcgggcg
     1081 tgcactgctt cgtgatggac gcgctcgaga ccgcgcgcct tgccgaactg atcgcattcg
     1141 tccgcgccag gttcgccacc gtcgatgcgg tgctggtggc gcacggtacc ctgcccgacc
     1201 aggcacgctg cgaaagctcg accgcggaca ccatcgcggc gatcgaagtg aacggcgtat
     1261 cgacggtggc gatactcgcc gcatttgccc agctgctgga agcacagaag cgcggcacac
     1321 tcgccgtgat cggttccccg gcgggcgacc gtggccgcgc cagcaactac acctacggtg
     1381 cggcgaaggc gctggttcac actttcgcgg acggtctgcg gcatcgcctg tggcgatgcg
     1441 gagtggccgt cgtgacgatc aagccgggtt tcaccgacac gccgatgacg gcggcgaagc
     1501 agaagggcgg gccgctgtgg gcgaccccgg cccgcgtggc gcgcgacatc gtcgcggcga
     1561 tcgaccaggg ccgcccggtc gtctacacgc cgtggttctg gcgctggatc atgctcgcga
     1621 tcgtgcacct gcctcagcgg ctcttcgtgc ggacgcggct gtagcggagg ctcggctcgc
     1681 ccgttgacgg tccggcgcgg tgacgtctga tcgggtgtgc gactcatcgg ctgccgtggc
     1741 aagggcaggc gctgcgcgcg t
//
LOCUS       DBNHBY010000420         8880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1040008, whole genome shotgun sequence.
ACCESSION   DBNHBY010000420 DBNHBY010000000
VERSION     DBNHBY010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1040008"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC16HB_10425"
     CDS             complement(<1..212)
                     /locus_tag="AC16HB_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5607247.1"
                     /translation="MNWAFWRSKDTARAARPDADDTQDPTAALHVRTRRRLIGAAALL
                     LAAVIVLPMVLDPTPRPVPDTVAIDIP"
     gene            complement(237..1547)
                     /gene="folC"
                     /locus_tag="AC16HB_10430"
     CDS             complement(237..1547)
                     /gene="folC"
                     /locus_tag="AC16HB_10430"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5607248.1"
                     /translation="MTPVAGRPATTPLNSTPSALDRWLERIERLHHRPIDLTLERSRA
                     VARRLGIEFACPAIVVGGTNGKGSTCAMLDSILRAAGYRVALYTSPHLLCFNERARID
                     GKIAADEELIAQFEAVEAARADVSLTFFEFTTLAILRLFQQSHLDALVLEIGLGGRLD
                     TVNLVDSDCAIVTSIDLDHMEYLGPTREQIGFEKAHIFRAGRPAICSDPQPPPSLIEV
                     AGRIGADLWRFGRDFNYQGDRQQWSYGGRALRRSSLPYPALRGANQLLNASGALAALE
                     ALSDRLPVSQQAVRQGLLTVEIAGRFQVLPGQPAVILDVAHNPHAAAVLADNLDNMGF
                     YPATHAVFGMLRDKDIGGVVKKIGARVDHWYIGATAGARGCRAEDVAQIVRGANPGRA
                     SVETFESIAAAYGAAAERAAPEDRMIVFGSFSTVAEVMRARTRH"
     gene            complement(1552..2418)
                     /gene="accD"
                     /locus_tag="AC16HB_10435"
     CDS             complement(1552..2418)
                     /gene="accD"
                     /locus_tag="AC16HB_10435"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR5607249.1"
                     /translation="MSWIEKLLPPRIKQGGTRRSMPEGVWVKCPGCEAALYRAELERS
                     LQVCPKCSHHLRIGARARLDALLDAEGRYEIGQEVLPVDALKFKDSKKYPDRLQDAMQ
                     ETGETDALVVVGGSIRTLQVVVACFEFEFMGGSMGSVVGERFVRGVQTALEQKVPFIC
                     FTATGGARMQEGLLSLLQMAKTSAMIAKLADARLPFISVLTDPTMGGVSASFAFMGDV
                     VIAEPKALIGFAGPRVIEQKVREKLPEGFQRAEFLLTKGAVDMIVDRRQMRDEIAHLL
                     ALLTRQPADAIA"
     gene            complement(2415..3236)
                     /gene="trpA"
                     /locus_tag="AC16HB_10440"
     CDS             complement(2415..3236)
                     /gene="trpA"
                     /locus_tag="AC16HB_10440"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR5607250.1"
                     /translation="MSRIAQRFADLARRGRKGLIPYIAAGDPTPELTVPLMRALAEAG
                     ADVIELGVPFSDPMADGPVIQRAAERAIRNGVGLKRTLALVAEFRKSDTTTPVVLMGY
                     ANPIEHMGRSEFARAASAAGVDGVLVVDYPPEECEEFAQAMRAVGIDPIFLLAPTSTD
                     DRIEQVAKVASGYLYYVSLKGITGAGHLDVGAVGARIPAIKARAKVPVGVGFGIRDAK
                     SAQAIARIADAVVIGSRIIEEIEAAPRERAVDAVRDLVAGIRRAMDDVSPLQEAA"
     gene            complement(3233..4483)
                     /gene="trpB"
                     /locus_tag="AC16HB_10445"
     CDS             complement(3233..4483)
                     /gene="trpB"
                     /locus_tag="AC16HB_10445"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR5607251.1"
                     /translation="MKPYDLPDARGHFGPYGGIFVAETLMHALAELNEAYARYRVDPE
                     FVAELQRELKHFVGRPSPIYHAKRWSEQLGGAQIFLKREDLNHTGAHKVNNTVGQALL
                     AKRMGKPRVIAETGAGQHGVATATVAARFGMQCVVYMGEEDVGRQAANVYRMKLLGAE
                     VVPVASGSRTLKDALNEAMRDWVTHVENTFYIIGTVAGPHPYPMMVRDFNSVVGKECL
                     QQMPEIAGRQPDYVIACVGGGSNAMGIFYDYIPHENVKLVGVEAAGEGLTTMRHAASL
                     LRGSPGVLHGNRTYLLQDEDGQITETHSISAGLDYPGVGPEHAWLKDSGRAEYVGATD
                     DEALRAFHDCCRIEGIIPALESSHALAHAVKLAPTLPKDRILLVNLSGRGDKDLHIVA
                     EASGLEFFCRASCREATAFWKQHR"
     gene            complement(4480..5124)
                     /locus_tag="AC16HB_10450"
     CDS             complement(4480..5124)
                     /locus_tag="AC16HB_10450"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR5607252.1"
                     /translation="MSRTRVKICGLTRNEDVAAAVRAGADAVGFVCYESSPRFVPLTA
                     LESLARALPAFVSPVLLFVNASPAAVEAALARVPNALLQFHGDESAADCERFRRPYLR
                     ALRVARSTDLLDCERTFALASALLVDAPAQGFGGGGVAFDWSLLPSAQARTKPLVLAG
                     GLNETNVTQAIEQVRPFAVDVSSGVEFSRGVKSAERIHRFVAAVRAADRELQQA"
     gene            complement(5121..5900)
                     /gene="truA"
                     /locus_tag="AC16HB_10455"
     CDS             complement(5121..5900)
                     /gene="truA"
                     /locus_tag="AC16HB_10455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5607253.1"
                     /translation="MRLALGLQYDGAGFCGWQTQPNRGAVQDVLETALRQFSGRAIET
                     TCAGRTDAGVHATYQVVHFDTDVERSLDAWVRGVNRWLPATLAVRWALSVTDDFHARY
                     SARARRYDYWLLSDPVRAPLLARRVGWVFRPLDVESMRAAARSLVGVHDFSSFRSSEC
                     QAASPVRELRTLEVSTHGRLLRFRLVANAFLHHMVRNLVGTLVYVGLGRQPISWIGEL
                     LDARDRRLAAPTFSAAGLYLSAVDYDSVFGFPQAEEFAPLA"
     gene            complement(5961..8825)
                     /locus_tag="AC16HB_10460"
     CDS             complement(5961..8825)
                     /locus_tag="AC16HB_10460"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR5607254.1"
                     /translation="MATNNSSSRSARSALALAVSLAFATPVVDAASLGRLTVYSALGQ
                     PLRAEVEVNSVTPQEAASLAVRLASMAAFRQANLEFNPALSSLRFALDKRGDNRYVVR
                     VSSQSAVNEPFLDLLLELNSSAGRVLREYTVLLDPPALKAVPEVVAPVAQAPSTPAPA
                     PSAAPATAPVPAAAPSAAAPPAPAPSPRAAAGSTYSVQRGDTLGKIAQQTKPPAASLD
                     QMLVSLFRANPDAFVAKNMNNLRAGSTLRIPAESETLAMGAVDARREVAAQSADFAQY
                     RSRLAQAAGTVGTTTAPAAGQGRVTAKVEDKSAPARTGDQLRIAKAESGAPGQAAASK
                     AGDEAVQRERQIKDLQQRIAELEQTNQKLQQALELQSKAGATAQKTAEAKAGTAPTAA
                     PAPAPVAAPTPPAAPEPAAKAPAPAEPPKVEPAKAEPAKVEPPKAEQPKVEAPKAEAP
                     KPAPAKEAPAEQPGFLRSLLGDSAGYLIGGLAAIVVALLGVAAYRRRKSADQKGDFEM
                     VEGLRGNSLFGQTGGQSVDTAATSTFNSSFIPATSQLDSNEVDPVAEADVYIAYGREE
                     QAEEILKEALRMQPDRHPARAKLLEIYSRRSDKASFNAVAHELKERTGGMGEEWDRVV
                     KLGRALDPGNALYGGGSAAAEKAERGPMTELRLSPSTSIRATTTGGPSTALPISEIES
                     PATVPAKDMPSLDMRESTVGRPTEAPVSKAGGDERMPDTYQPQDSRLGVETKLPLESM
                     EAPQTKIAREDEPTQLAGLDFELEAPLTTMKRDAPAKTDAPAVPAFDMNFEPPKTLPA
                     DKLPLLPKLDLDLPGKKVEPKPPSVAPIAMPARETMRPAATVPGFDDTLSRPSLIGEV
                     GALPEERAPRLLSNTDQATVPLIDFDLSGADVELGGRKTETPIGSPLAAQMATKLDLA
                     RGYIDLGVKDGARELLEEVMRDGTREQRQSAVELMKQIEN"
BASE COUNT         1431 a         3119 c         2863 g         1467 t
ORIGIN      
        1 ggaatgtcga tcgccaccgt atcgggcacg ggccgcggcg tcggatcgag caccatcggc
       61 agcacgatca ccgccgccaa caggagcgcg gcagcgccga tcagtcgccg acgcgtgcgg
      121 acatgcagcg cggcggtcgg atcctgcgtg tcatccgcgt cgggtctcgc cgcgcgggca
      181 gtgtccttcg agcgccagaa ggcccagttc atggattgga tcggagggta cgaaggtcag
      241 tgacgcgttc gcgcgcgcat cacttccgcg acagtggaaa aacttccaaa cacgatcatt
      301 ctatcctccg gcgccgcgcg ctccgcggcc gcgccatacg ccgccgcgat cgattcgaac
      361 gtctccaccg acgcgcgacc cgggttcgca ccacggacga tctgcgccac gtcttcggcg
      421 cggcagccgc gtgcacccgc ggtggcgccg atataccagt gatcgacgcg cgcgccgatc
      481 ttcttcacga cgccgccgat gtccttgtcg cgcagcatgc cgaacactgc gtgcgtggcc
      541 ggatagaacc ccatgttgtc gaggttgtcc gccaggacgg ccgccgcgtg cgggttgtgt
      601 gcgacatcga ggatcacggc cggttgtccc ggcagcacct ggaagcgccc ggcgatctcg
      661 acggtcagca gaccctgccg tacggcctgc tggctgaccg gcagccgatc cgacagcgct
      721 tcgagcgccg ccagcgcgcc gctggcattg agcaactggt tcgccccacg cagcgcgggg
      781 tacggcaggc tgctccggcg caatgcgcgt ccgccatagc tccattgctg gcgatcgccc
      841 tggtaattga aatcgcgacc gaaccgccac agatcggcgc cgatcctgcc cgcaacttcg
      901 atcagcgatg gcggcggctg cggatcggaa cagatcgccg ggcgcccagc gcgaaagatg
      961 tgcgcctttt cgaagccgat ctgctcccgc gtcggcccga ggtactccat gtggtccaga
     1021 tcgatcgagg tgacgatcgc gcaatcggaa tcgacgaggt tcaccgtgtc gagccggccg
     1081 ccgaggccga tctcgagcac gagggcatcg agatggcttt gctggaacaa ccgcaggatc
     1141 gccagcgtgg tgaattcgaa gaaagtcagc gacacgtccg cgcgcgccgc ctcgacggct
     1201 tcgaactgcg cgatcagttc ctcgtcggcg gcgatcttgc cgtcgatgcg cgcgcgctcg
     1261 ttgaagcaca gcagatgcgg cgacgtatac aaggcgacgc gatagccggc cgcgcgcagg
     1321 atgctgtcga gcatcgcgca ggtcgatccc ttgccgttgg tgccgccgac cacgatggcc
     1381 ggacacgcga actcgatgcc cagccgccgc gcaacggcac gcgagcgctc cagggtcagg
     1441 tcgatcggcc ggtgatgcag gcgctcgatg cgttcgagcc agcgatccag agcggaagga
     1501 gtgctgttca gaggagtcgt ggccggccgc cccgcaaccg gcgtcatccg tctaagcgat
     1561 cgcgtccgcg ggctgtcgtg tcagaagcgc cagcagatgc gcgatctcgt cgcgcatctg
     1621 gcgacggtcg acgatcatgt cgaccgcacc cttggtcagc aggaactcgg cgcgctggaa
     1681 cccttcaggc agcttctcgc gcaccttctg ctcgatcacc cgcgggccgg caaagccgat
     1741 caacgccttc ggctcggcga tcaccacatc gcccatgaaa gcgaagctcg ccgacacgcc
     1801 acccatggtc ggatcggtca gcaccgagat gaacggcagc cgcgcatcgg cgagcttcgc
     1861 gatcatcgcg gaggtcttcg ccatctgcag cagcgacagc agaccttcct gcattcgcgc
     1921 gccaccggtg gcggtgaagc agatgaaggg caccttctgc tccagcgccg tctgcacgcc
     1981 gcgcacgaag cgctcgccga cgaccgagcc catcgaaccg cccatgaact cgaattcgaa
     2041 gcaggcgacc accacctgca gcgtgcgaat cgacccgccg acgacgacca gcgcatcggt
     2101 ctcgccggtc tcctgcatcg cgtcctgcag ccgatccgga tacttcttgc tgtccttgaa
     2161 cttcagcgca tcgaccggca gcacttcctg gccgatctcg taccgtccct cggcatcgag
     2221 cagcgcgtcg agacgcgcac gcgcaccgat gcgcaggtgg tggctgcact tcgggcacac
     2281 ctgcagcgag cgttccagtt cggcgcggta cagtgccgcc tcgcagcccg ggcacttgac
     2341 ccagacgccc tccggcatcg acctgcgcgt gcccccctgc ttgatgcgcg gcggcagcag
     2401 cttctcgatc cagctcatgc ggcctcctgc agcggcgata catcgtccat cgcgcgacgg
     2461 attccggcca ccaggtcgcg cacggcatcg acggcgcgct cgcgcggcgc cgcttcgatt
     2521 tcctcgatga tccggctgcc gatgacgacc gcgtcggcga tgcgcgcgat cgcctgcgcg
     2581 ctcttcgcgt cgcggatgcc gaagccgaca ccaaccggca ccttcgccct ggccttgatc
     2641 gccggaatgc gcgcaccaac cgctccaacg tcgagatgtc ctgcgccggt gatgcccttc
     2701 aggctcacgt agtacagata gccggacgcc accttggcca cctgttcgat gcggtcgtcg
     2761 gtcgaggtcg gcgccaacag gaagatcgga tcgattccga ccgcacgcat cgcctgggcg
     2821 aattcttcgc actcctcggg cggatagtcg acaaccaaca ctccgtccac gccggccgcg
     2881 ctcgcggcac gcgcgaattc gctgcgtccc atgtgctcga tcgggttggc gtagcccatc
     2941 agaaccaccg gcgtcgtcgt gtcggacttg cggaactcgg caaccagcgc cagcgtgcgc
     3001 ttcagcccga caccgttgcg gatcgcgcgc tcggccgcgc gctggatcac cggaccatcg
     3061 gccatcggat cggaaaacgg cacgccgagt tcgatgacgt ccgcgcccgc ttctgccagc
     3121 gcacgcatca gcggcaccgt cagctccggc gtcggatcgc cggcagcgat atacggaatc
     3181 aatcccttgc ggccgcgccg cgcgagatcg gcgaagcgct gcgcgatgcg gctcatcgat
     3241 gctgtttcca gaatgcggtc gcctcacggc acgaggcgcg gcagaagaac tcgagaccgc
     3301 tcgcctcggc aacgatgtgc aaatccttgt caccgcggcc gctgaggttc acgagcagga
     3361 tcctgtcctt gggcagcgtc ggcgccagct tgaccgcgtg cgcgagtgcg tggctcgact
     3421 ccagtgccgg gatgatgcct tcgatccggc agcaatcgtg gaatgccctc agcgcctcgt
     3481 catcggtcgc gccgacatac tccgcacgac cgctgtcctt gagccatgcg tgctcaggac
     3541 cgacaccggg gtagtcgagc ccggcggaga tcgaatgggt ctcggtgatc tggccatcct
     3601 cgtcctgcag caagtacgtc cggttcccgt ggagcacgcc gggcgatccg cgcagcaggc
     3661 tggccgcgtg gcgcatcgtg gtcaggccct cgcccgcagc ttcgacgccg acgagcttca
     3721 cgttttcgtg cggaatgtag tcgtagaaga tccccatcgc gttgctgccg ccgccgacgc
     3781 acgcgatcac gtagtcgggc tggcggccgg cgatctcggg catctgctgc aggcactcct
     3841 tgccgacgac cgagttgaag tcgcgcacca tcatcggata cggatgcggc cccgcgacgg
     3901 ttccgatgat gtaaaaggtg ttctcgacgt gggtcaccca gtcgcgcatc gcctcgttca
     3961 gcgcgtcctt cagggtgcgc gacccgctcg ccaccggcac cacctcggcg ccgagcagct
     4021 tcatccggta gacattcgcg gcctggcgcc cgacgtcctc ctcacccatg taaacgacgc
     4081 actgcatgcc gaagcgcgcg gcgaccgtcg ccgtggcgac accgtgctgc ccggcgccgg
     4141 tttcggcgat cacgcgcggc ttgcccatcc gcttcgcgag cagcgcctgg ccgaccgtgt
     4201 tgttgacctt gtgcgcgccg gtgtggttca ggtcctcgcg cttgagaaag atctgcgcgc
     4261 cgccaagctg ttcgctccag cgcttcgcgt gatagatcgg gctcggccgg ccgacgaaat
     4321 gcttcagttc gcgctgcaat tcggcgacga actcgggatc gacgcggtat ctcgcatatg
     4381 cctcgttcaa ctcggcgagc gcatgcatca gcgtctcggc gacgaagatt ccgccgtagg
     4441 ggccgaaatg gccacgcgcg tcgggcagat cgtagggttt catgcctgct gcaattccct
     4501 gtccgccgcg cgcacggcag cgacgaaccg atggatcctt tccgcgctct tgacgccgcg
     4561 cgagaactcg acgccgctgc tcacgtcgac tgcgaacggc cgcacctgct caatcgcttg
     4621 cgtgacattc gtctcgttca agccgccagc cagcacgagt ggtttcgtcc gcgcctgcgc
     4681 cgacggcagc agcgaccagt cgaaagcgac accgccgccg ccgaatcctt gcgccggcgc
     4741 atcgaccagc aacgcactgg ccaaggcaaa ggtgcgctcg cagtctaaca aatccgtgct
     4801 gcgtgccacg cgcagcgcgc gcaggtacgg gcgccggaaa cgctcgcaat cggccgccga
     4861 ttcgtcgccg tggaactgca gcagcgcatt cggcacgcgc gccagcgccg cttcgacggc
     4921 ggcggggctc gcattgacga acagcagcac cggggacacg aacgccggca acgcgcgcgc
     4981 gagactctcg agtgcggtga gcggcacgaa gcgcggactg gactcgtagc agacgaaacc
     5041 gaccgcatcg gcgccggccc ggaccgccgc ggcgacgtcc tcgttacggg tcaaaccaca
     5101 gatcttgacg cgggtcctgc tcatgcgagc ggcgcgaact cctcggcctg cggaaaaccg
     5161 aaaaccgaat cgtagtcgac tgcgctcagg tacaggcccg ccgcggaaaa cgtcggcgct
     5221 gccagccggc gatcgcgcgc gtcgagtagt tcgccgatcc agctgatcgg ctgacgtccg
     5281 agtccgacgt acaccagcgt acccacgagg ttgcgcacca tgtgatgcag gaacgcgttg
     5341 gcgaccagcc ggaagcggag caggcggccg tgcgtcgaca cttcgagcgt ccgcagttcg
     5401 cgcaccggcg acgccgcctg gcattcggac gaccggaacg aactgaagtc gtgcacgccg
     5461 accagcgaac gcgcggccgc gcgcatcgat tcgacgtcga gcggccgaaa gacccagccg
     5521 acgcggcgcg ccagcagcgg cgcgcgcacc ggatcgctga gcagccagta atcgtatcgg
     5581 cgcgcgcgcg cggagtagcg cgcgtggaag tcgtcggtca ccgacagcgc ccaacgcacg
     5641 gccagggttg ccggcagcca gcgattgacg ccgcgtaccc atgcgtcgag cgaacgctcg
     5701 acgtccgtgt cgaagtgaac cacctggtag gtcgcatgca cacccgcgtc ggtacgcccg
     5761 gcgcacgtcg tctcgattgc ccggcccgag aactggcgga gcgcggtctc gagcacatcc
     5821 tgcaccgcgc cccggttcgg ctgcgtctgc cagccgcaga accccgcacc gtcgtattgc
     5881 aggccaagcg caagacgcat cgcgaaggag aaaatgaaaa gccggcgcgg ctgctaccgc
     5941 gccggcttcg tcgcgtcgcg tcagttctcg atctgcttca tcaattcgac cgccgactga
     6001 cgctgctcgc gcgtgccgtc gcgcatcact tcctcgagca gttcgcgcgc accgtccttg
     6061 acgcccagat cgatgtagcc acgcgcgagg tcgagcttgg ttgccatctg tgccgcgagc
     6121 ggactgccaa ttggagtctc ggtcttccgt ccgccgagct cgacgtcggc cccgctgagg
     6181 tcgaaatcga tcagcggcac cgtcgcctgg tcggtgttcg acaacagacg cggagcccgc
     6241 tcctccggca gcgcgccgac ttctccgatc agcgacggac gcgacagcgt gtcgtcgaat
     6301 cccggcacgg tggccgcagg ccgcatcgtc tcgcgcgccg gcatcgcgat cggcgccacc
     6361 gagggcggct tcggctccac cttcttgccc ggcaggtcga ggtcgagctt tggcagcagt
     6421 ggcaacttgt cggccggcaa cgtctttggc ggctcgaagt tcatgtcgaa ggcaggcaca
     6481 gcgggcgcat ccgtcttcgc cggtgcatcg cgcttcattg tcgtgagcgg cgcttcgagt
     6541 tcgaagtcga gcccggccag ctgagtcggc tcgtcttctc tggcgatctt cgtctgcggc
     6601 gcctccatgg actcgagcgg caacttggtt tcgacaccga gccgcgaatc ctgcggctga
     6661 tacgtgtccg gcatccgctc atcgcccccg gccttggaaa ccggcgcctc ggtcggcctg
     6721 cccaccgtgg attcacgcat gtccaggctc ggcatatcct tcgcgggcac cgtcgccggg
     6781 ctctcgatct ccgaaatcgg caatgcggtg gacgggccgc ccgtcgtcgt ggctcggatc
     6841 gacgtgctgg ggctgagccg cagttccgtc atcggcccac gttccgcctt ctcggccgca
     6901 gcagatccgc cgccgtacaa cgcattgccc ggatcgaggg cgcggccaag cttgaccacg
     6961 cgatcccact cctcgcccat gccgccggtg cgctccttca gttcgtgcgc caccgcattg
     7021 aacgaggcct tgtcgctgcg acgcgagtag atctcgagca gcttggcgcg ggccgggtgc
     7081 cgatccggct gcatccgcag cgcctccttg aggatctctt cggcctgctc ttcgcggccg
     7141 taggcgatgt aaacatccgc ctcggctacc gggtcgacct cgttcgagtc gagctgcgac
     7201 gtggccggaa tgaagctcga attgaacgtg ctcgtggccg ccgtatcgac actctgaccg
     7261 ccggtctgtc cgaacagcga attgccgcgc agtccctcga ccatctcaaa gtcgcccttc
     7321 tggtccgccg acttgcgacg gcgataggca gccacaccca gcaacgccac aacgatcgcg
     7381 gccagcccgc cgatcaggta gccggcggag tcgcccagca ggctgcgcag gaagcccggc
     7441 tgctcggccg gcgcttcctt cgcaggcgcg ggcttcggcg cttccgcctt gggcgcttca
     7501 accttcggtt gctcggcctt tggcggttcg accttcgccg gttccgcctt ggcgggctcg
     7561 accttcggcg gttcagcggg cgcgggcgcc ttggccgcgg gctcgggtgc cgcggggggt
     7621 gtcggggccg cgaccggcgc gggtgccggt gcggctgtcg gcgccgtacc tgctttcgcc
     7681 tcggccgtct tctgcgccgt tgcaccggcc ttgctctgca gttcgagcgc ctgttgcagc
     7741 ttctggttgg tctgttccaa ctccgcgatg cgctgctgca ggtccttgat ctgccgttcg
     7801 cgttgaaccg cctcgtcgcc agccttcgaa gcagcggcct ggcccggcgc gccgctttcc
     7861 gctttcgcga tccgcaactg atcaccggtg cgtgccggcg cgctcttgtc ctcgaccttg
     7921 gcggtgactc gaccctgtcc agccgcagga gccgtcgtcg ttccgaccgt gccggcagcc
     7981 tgcgcaaggc ggctgcggta ctgggcgaaa tcggcgctct gcgcggcaac ttcgcgacgt
     8041 gcgtcgacgg cacccatcgc gagcgtctcg ctttccgccg gaatgcgcag cgtgctgccc
     8101 gcgcgcaggt tgttcatgtt cttcgcgacg aatgcgtccg gattggcgcg gaagagcgac
     8161 accagcatct gatccaacga tgccgccgga ggtttggtct gctgcgcaat cttgccgagc
     8221 gtgtctccgc gctgcaccga gtacgtcgaa cctgccgcag cgcgcggcga aggagccggc
     8281 gccggagggg ctgcggccga cggggcagcg gccgggaccg gcgcggttgc cggcgctgcc
     8341 gacggcgccg gagcgggcgt cgacggcgct tgtgcaaccg gggcaacaac ttccggcaca
     8401 gccttcagcg ccggcggatc gagcagcacc gtgtactcgc gcagcacgcg gccagccgat
     8461 gaattcagtt ccagcagcag atcgaggaac ggctcgttca ccgcagactg tgacgagacc
     8521 ctgactacgt aacggttgtc accacgcttg tcgagcgcaa accgcagcga cgacagcgca
     8581 ggattgaact cgaggttggc ctgccggaag gcggccatcg acgccagccg caccgcgagc
     8641 gaagcggcct cctggggcgt gaccgaattg acctcgacct ccgcgcgcag aggctgaccc
     8701 agcgctgagt agaccgtcag tcgacccagg ctcgcggcat cgacgacggg tgtcgcaaac
     8761 gcaaggctga ccgctaatgc gagcgcggac cgcgccgatc ttgaactcga attgttcgtt
     8821 gccacggctg cctctcaagg gcggtcaagt ttgatttcga acttaacatc agtgacttag
//
LOCUS       DBNHBY010000421         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1311538, whole genome shotgun sequence.
ACCESSION   DBNHBY010000421 DBNHBY010000000
VERSION     DBNHBY010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1311538"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC16HB_10465"
     CDS             <1..345
                     /locus_tag="AC16HB_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5607255.1"
                     /translation="EAGQGVEIALSVPKEGTIIWFDMFAIPADAPHPNNAHQFINFMM
                     RPEIAAANSNSVHYANGVGASKDLIDPAVRNDPGVYPPPETMDKLFPNLARSPEFTRQ
                     LNRTWTRFTTGK"
     gene            516..1670
                     /locus_tag="AC16HB_10470"
     CDS             516..1670
                     /locus_tag="AC16HB_10470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607256.1"
                     /translation="MAANPPAARPAQKKFEPWNDPDAKPYVRIEKVTKKFGEFIAVDD
                     VSLDIYKKEIFCLLGASGCGKTTLLRMLAGFERPTSGKIYIDGVDMTTIPPYERPVNM
                     MFQSYAVFPHMTVDQNVAFGLKQEGLAKSEIADRVADMLKLVRLDQFGKRKPHQLSGG
                     QRQRVALARALVKKPKLLLLDEPLAALDKKLREHTQFELINIQETLGVTFVVVTHDPE
                     EAMTLSTRVGLMNHGEIIQVGTPANMYEYPNSRFVAEFIGSVNMFEGRLVEDEPSYVR
                     IECDDLEVPIFVDHGISSSPGATVWAAIRPEKIQIVRDKPAEPDNWVLGTVQDIAYMG
                     DVSIYLVRLASGKVVRVTQPNVYRHAEDAPTWEDQVYLTWHGSSPVVVLK"
     gene            1667..2578
                     /locus_tag="AC16HB_10475"
     CDS             1667..2578
                     /locus_tag="AC16HB_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5607257.1"
                     /translation="MTSLLKRIAVRGQTLVTSVPYLWLIVFFLIPFLIVLKISFSTTQ
                     IAMPPYEPLLHWTGEKLVQIKLNLGNFAFLVEDDLYWRAYLNSIKVALVSTLFCLLIG
                     YPTAYAIARSNPATRNILMLLVVLPFWTSFLLRVYAWIGILKNNGLINNALMGLGIID
                     QPIQMLQTDFAVYVGIVYSYLPFMILPLYANLEKMDLTLLEAAADLGCRPWQAFLKVT
                     LPLSLPGIIAGCLLVFIPAVGEFVIPSLLGEPGMLMIGKVLWTEFFNNRDWPVASAVA
                     IALLLFLVIPIMLFQRAQDAEAGGVHR"
     gene            2575..>2748
                     /locus_tag="AC16HB_10480"
     CDS             2575..>2748
                     /locus_tag="AC16HB_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HIR5607258.1"
                     /translation="MNNGRSLFRALALILGFAFLYIPIASLIVYSFNESKLVTVWGGF
                     STKWYGELLRNQQI"
BASE COUNT          496 a          920 c          846 g          486 t
ORIGIN      
        1 gaagccgggc agggcgtcga gatcgccctc agcgtgccga aggaaggcac catcatctgg
       61 ttcgacatgt tcgcgatccc ggccgacgcg ccgcacccga acaacgcgca ccagttcatc
      121 aacttcatga tgcgccccga aatcgccgcg gcgaactcca actccgtgca ctacgcgaac
      181 ggcgtgggcg cctcgaagga cctgatcgat ccggcggtgc gcaacgatcc gggcgtgtat
      241 ccgccgccgg agacgatgga caagctgttc ccgaacctgg cgcgttcgcc ggagttcacg
      301 cgacagctga accgcacctg gacgcgattc acgaccggta agtaagccgg gcggagccgc
      361 aaaggttcga tcgctgatgg ggcggccgga gcaatccggc cgcccctttt catgtggcgc
      421 cgcgctaccc atcgccgcgt cgctgagcta taaccgcgca gtcgcctgcg cccgaccgtc
      481 gcgcggacgg cacgacaggg agctcctata agaccatggc cgccaatccg cctgctgccc
      541 gccccgccca gaagaaattc gaaccctgga acgatccgga cgccaagccg tacgtccgca
      601 tcgagaaggt gacgaagaag ttcggggagt tcatcgccgt cgacgacgtc tcgctcgaca
      661 tctacaagaa ggaaatcttc tgcctgctcg gcgcatcggg ctgcggcaag accacgctgc
      721 tgcgcatgct cgccggcttc gagcggccga cctccggcaa gatctacatc gacggcgtcg
      781 acatgacgac cattccgccg tacgagcggc cggtcaacat gatgttccag tcgtacgcgg
      841 tgttcccgca catgacggtc gaccagaacg tcgcgttcgg cctcaagcag gaaggactcg
      901 cgaagagcga gatcgccgac cgggtcgcgg acatgctcaa gctcgtccgg ctcgaccagt
      961 tcggcaagcg caagccgcat cagctgtcgg gcggccagcg gcagcgtgtc gcgctggcgc
     1021 gcgcactggt caagaagccg aagctgctgc tgctcgacga gccgctggcc gcgctcgaca
     1081 agaaactgcg cgagcacacg cagttcgagc tgatcaacat ccaggagacg ctcggcgtca
     1141 ctttcgtggt cgtgacgcac gacccggaag aggcgatgac gctgtcgacg cgcgtcggcc
     1201 tgatgaacca cggcgagatc atccaggtcg gcacgccggc caacatgtac gaatacccga
     1261 actcgcgctt cgtcgccgag ttcatcggct cggtcaacat gttcgagggc aggctggtcg
     1321 aggacgaacc gtcgtacgtg cgcatcgagt gtgacgacct cgaagtgccg atcttcgtcg
     1381 accacggcat cagttcttcg ccgggcgcga cggtgtgggc cgcgatccgt ccggagaaga
     1441 tccagatcgt ccgcgacaag cccgccgagc ccgacaactg ggtgctcggc acggtgcagg
     1501 acatcgcgta catgggtgac gtgtcgatct acctggtgcg gctcgcctcc ggcaaggtcg
     1561 tgcgcgttac acagccgaac gtgtaccggc acgcggaaga cgcccccacg tgggaagacc
     1621 aggtctacct gacctggcac ggctcgagcc cggtggtggt gctgaagtga ccagcctgct
     1681 caagcgcatc gcggtgcgcg gccagacgct cgtcacgtcc gtgccctacc tgtggctgat
     1741 cgtctttttc ctgatcccgt tcctgatcgt cctgaagatc agcttctcga cgacgcagat
     1801 cgcgatgccg ccgtacgagc cgctgctgca ctggaccggc gagaagctgg tgcagatcaa
     1861 gctcaacctc ggcaacttcg cgttcctggt ggaggacgac ctgtactggc gcgcatacct
     1921 caattccatc aaggtcgcgc tggtctcgac gctgttctgc ctgctgatcg gttacccgac
     1981 cgcctacgcc atcgcgcgca gcaatccggc gacccgcaac atactgatgc tgctcgtcgt
     2041 gctgccgttc tggacgtcgt tcctcctgcg cgtctacgcg tggatcggca tcctcaagaa
     2101 caacggcctg atcaacaacg cactgatggg gctggggatc atcgaccagc cgatacagat
     2161 gctgcagacc gacttcgccg tctatgtcgg catcgtctac tcgtacctcc cgttcatgat
     2221 cctgccgctg tacgccaacc tcgagaaaat ggacctcacc ctgcttgagg ccgcggcgga
     2281 cctcggctgc cggccgtggc aggcgttcct gaaggtcacg ctgccactgt cgctgcccgg
     2341 cattatcgcg ggctgcctgc tggtgttcat ccccgcggtc ggcgagttcg tcattccgtc
     2401 cctgctcggc gaaccgggca tgctcatgat cggcaaggtg ctgtggaccg agttcttcaa
     2461 caaccgtgac tggcccgtcg ccagcgcagt ggccatcgcg ctgctgctgt tcctggtgat
     2521 cccgatcatg ttgttccagc gtgcccagga cgccgaggcc ggcggggtgc accgatgaac
     2581 aacggtcgca gcctgttccg cgcgctggcc ctgatcctgg gcttcgcgtt cctgtacatc
     2641 ccgatcgcgt cgctgatcgt ctactccttc aacgagtcga agctcgtgac ggtctggggc
     2701 gggttctcga ccaagtggta cggcgagctg ctgcgcaacc agcagatc
//
LOCUS       DBNHBY010000422         2405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1514384, whole genome shotgun sequence.
ACCESSION   DBNHBY010000422 DBNHBY010000000
VERSION     DBNHBY010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1514384"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /locus_tag="AC16HB_10485"
     CDS             <1..1929
                     /locus_tag="AC16HB_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5607259.1"
                     /translation="IGLLIDDAIVVRENIVRHIQMGKDHYTAAREGTDEIGLAVLATT
                     FSIVAVFVPIAFMSGIVGKFFYPFGMTVVVAVLVSLFVSFTLDPMLSAVWRDPPQGAK
                     DLRLIGPLLRRFDGWVESWHRIYDRLLRAALERRKTSLAIAAASFLVSLPIAGFVGGE
                     LMPVADQSFTSVGLTLPVGSSLEYADERVKRVEAVLREFPEIESIDTSIGTEGARNNG
                     RINLKLVPRSERKRSQKQIEEAIRKRMAGIPGVQLTVGFQGAIYVALLGNNDAEMKRV
                     MADFKSKVAQIRGVTDIDVSIKEGTPALSVRLKPELAAEYGITHAQLGSTLRALVGGE
                     NSGYWLAPDGQNYEVITQLPRCNRTVIDDISNLNIATGRMMADGQPEVVPLRAIATIE
                     RTFNPDNIRRQDLQRRVALFANVEGRPAGDAGKEVQELVQSYQLPPGLRFDVGGQIRD
                     QQEVSAAIGGALLLAVIFIYIVLASQFGSFIQPLAIMASLPLSIVGVMLALLVTNTTL
                     NIFSMIGIVFLMGLVTKNAILLVDFANQGQRKGMDRLDALLAAGQTRLRPILMTTAAM
                     IFGMLPLAIGVGEGAEQQAPMGRAIIGGVITSTLLTLVVVPVIYSYLDGWEKRWFRRR
                     VPASGATVLTHQPAERER"
     gene            1952..>2405
                     /locus_tag="AC16HB_10490"
     CDS             1952..>2405
                     /locus_tag="AC16HB_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607260.1"
                     /translation="MNERRRGAPLSEDPDSRLVRRGWLAWAVAAVLIAWLAWRDRAHA
                     AEAMTSWILAAPALAWWLLWPLARGTIALWDWMRARPYAAWNGRYYEFNGRQIRILFD
                     GDALWIVADDVFDVLGLTGRARDPEHVRQIGGRDGLVRLPGSTLFAFTE"
BASE COUNT          375 a          778 c          840 g          412 t
ORIGIN      
        1 atcgggctgc tgatcgacga tgcgatcgtg gtgcgggaga acatcgtgcg ccacatccag
       61 atgggcaagg accactacac ggccgcgcgc gagggcaccg acgagatcgg cctggccgtg
      121 ctggccacca cgttctccat cgtcgcggtg ttcgtgccga tcgcgttcat gagcgggatc
      181 gtcggcaagt tcttctatcc gttcggcatg acggtcgtgg tcgcggttct ggtgtcgctg
      241 ttcgtcagct tcacgctcga cccgatgctg tcggcggtgt ggcgcgatcc gccgcagggc
      301 gcgaaggacc tgcggctgat cggaccgctg ctgcgtcgct tcgacggctg ggtcgagtcg
      361 tggcaccgca tctacgaccg cctgctgcgc gccgcgctcg aacggcgcaa gacctcgctc
      421 gcaatcgctg ccgcgagttt cctcgtcagt ttgccgattg cgggcttcgt cggcggggag
      481 ctgatgcccg tggccgacca gagcttcacc agcgtcgggc tgacgctgcc ggtgggttcg
      541 agcctcgaat atgccgatga acgcgtcaag cgcgtcgagg ccgtgctgcg cgagtttccg
      601 gagatcgaat cgatcgatac cagcatcggc acggagggcg cgcgcaacaa cggccgcatc
      661 aacctgaagc tcgtcccgcg cagcgagcgc aagcgctcgc agaagcagat cgaggaggcg
      721 atccgcaaac gcatggccgg catccccggt gtgcagctca cggtcggctt ccagggggca
      781 atctacgtgg cgctgctcgg caacaacgac gccgagatga agcgcgtgat ggcggacttc
      841 aagagcaaag tcgcgcagat ccgcggggtg accgacatcg acgtgtcgat caaggagggc
      901 acgcctgcgc tttcggtgcg gctgaagccg gaactggcgg ccgagtacgg catcacgcat
      961 gcgcagctgg gcagtacgct gcgcgcgctg gtgggcggcg agaactccgg ttactggctc
     1021 gcgcccgatg ggcagaacta cgaggtgatc acgcagttgc cgcgctgcaa ccgcaccgtg
     1081 atcgacgata tctcgaacct caacatcgcc accggccgca tgatggccga cggccagccc
     1141 gaggtcgtgc cgctgcgtgc gatcgcgacc atcgagcgca cgttcaatcc ggacaacatc
     1201 cgccgccagg acctgcagcg cagggtcgcg ctgttcgcca acgtcgaggg acggcccgcg
     1261 ggcgatgccg gcaaggaagt gcaggagctg gtgcagtcgt accaactgcc gccgggcctg
     1321 cgcttcgatg tcggcggcca gatccgcgat cagcaggaag tgagcgccgc gatcggcggc
     1381 gcgctgctgc tggcggtgat cttcatctac atcgtgctgg catcgcagtt cggcagcttc
     1441 atccagccgc tcgcgatcat ggcgtcgctg ccgctgtcga tcgtcggcgt gatgctggcg
     1501 ctgctcgtca ccaacaccac gctgaacatt ttctcgatga tcggcatcgt gttcctgatg
     1561 gggctcgtca cgaagaacgc gatcctgctg gtcgatttcg ccaaccaggg ccagcgcaag
     1621 ggcatggatc gactcgatgc gctgctcgct gccggacaga cgcggctgcg accgatcctc
     1681 atgacgaccg cggcgatgat cttcggcatg ctgccgctgg cgatcggggt gggagaaggc
     1741 gccgagcagc aggcgccgat gggccgcgcg atcatcggcg gcgtgatcac gtccacgctg
     1801 ctgacgctgg tcgtggtgcc ggtgatctac tcgtatctcg acgggtggga gaagcgctgg
     1861 ttccgccgtc gcgtcccggc cagcggcgcc actgtgctca cgcaccagcc ggccgaacgc
     1921 gagcggtagg agccggcgct acagtcggcg catgaacgag cgccggcgcg gtgcgccgct
     1981 gagcgaagac cccgactcgc gcctcgtccg ccggggctgg ctcgcgtggg cggttgcggc
     2041 cgtgttgatc gcgtggctcg cgtggcgcga tcgtgcccac gccgccgaag cgatgacttc
     2101 ctggattttg gctgcgcccg cgcttgcgtg gtggctgctg tggcccctgg cacgcggcac
     2161 gatcgcactc tgggactgga tgcgcgcgcg cccgtacgct gcgtggaacg gccgctacta
     2221 cgaattcaac ggccgccaga ttcgaattct tttcgacggc gatgcgctgt ggatcgtcgc
     2281 cgatgacgtg ttcgacgtgc tcgggctcac cggccgggcc cgcgatccgg agcacgtgcg
     2341 ccagatcggc ggccgcgacg gcctggtgcg gttgccgggc agtacgctct tcgcgttcac
     2401 ggagc
//
LOCUS       DBNHBY010000423         5107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_364605, whole genome shotgun sequence.
ACCESSION   DBNHBY010000423 DBNHBY010000000
VERSION     DBNHBY010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_364605"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="AC16HB_10495"
     CDS             <1..1250
                     /locus_tag="AC16HB_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR5607261.1"
                     /translation="SCSCADAEKVRGALATAVHARLSPALLLAFTAAFITLQPISTDL
                     YQAALPSIGTAFGVPVASVQLTLTVFIATFGVWQLLAGPLSDRFGRHPVALGGVVIYV
                     AASALCTLAPSMPWLIAGRALQAVGACSCLVAARAMVRDELTPEHGARVLAVSGTILG
                     ICVVIAPIAGGLLQAAFGWRATFGAMLALSSLLALATLLRLRETHRTPNRDALRAGPL
                     ARAYAEVARGPSWHAYTWPAVCSYAGLFSFISGGSFVLIRVLGVAPVYYGFCFSFIVS
                     GYIVGTLICRRSVPRRGMPWTMRRGGLLQFAAGGTLAGLALAGVHHPLALLLPQFVFL
                     IGHGLIQPVAQAGTMARFPHSAGVATAAMGLVMMVVAAAIGQWIGASYNGTVYPLTLT
                     IAACGTASALTTLTLVKRYGRVD"
     gene            1234..2175
                     /gene="miaA"
                     /locus_tag="AC16HB_10500"
     CDS             1234..2175
                     /gene="miaA"
                     /locus_tag="AC16HB_10500"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR5607262.1"
                     /translation="MAASIDAVLLLGPTACGKSALALELARTIPLEIVSVDSAQVYRG
                     MDIGTAKPSAQERARVPQHLIDVRDPSQPYSAADFVRDATRAIGEIKQRGKLPLAVGG
                     TMLYARALSAGLSKLPAADARVRARIGAHARELGWPALHVELAHVDPATAARLPPNDS
                     QRIQRALEIFELTGTPMSALLVASHAAPLRLQTIALLPADRAELHRRIEARFDAMLAV
                     GFLDEVRALMNRGDLDPDLPSMRSVGYRQAWRHLAERTSFTEFRAASIAATRQLAKRQ
                     ITWLRSMHEATRIDPFDPSTAIRLMQLLSTYAHSQRG"
     gene            complement(2231..2857)
                     /locus_tag="AC16HB_10505"
     CDS             complement(2231..2857)
                     /locus_tag="AC16HB_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607263.1"
                     /translation="MFAPSLRFTVSGAALALAAATLASPAVARSSGEELKFDMVRSAA
                     VVNAGCLPNARARVTIEPEGSVESMRVRLEGMPPATDFDFFVIQSPNAPFGLAWYQGD
                     IETNVHGVGQGRFIGRFSEETFIVAPGATAAPSVHNNACPDAILNPATGPIHTFHLGL
                     WFNSPADAVKAGCPGNVTPFNGEHNAGIQVLNTSNFPAPQGPLLQVKP"
     gene            complement(3074..3763)
                     /locus_tag="AC16HB_10510"
     CDS             complement(3074..3763)
                     /locus_tag="AC16HB_10510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5607264.1"
                     /translation="MGKTISFKRPDGQSVNGYLAEPAAAGAAGIVVIQEWWGLNDQIR
                     GVADKLAAAGYRALVPDLYRGQVALEANEAEHLMKGLNFGDAAGQDVRGALQHLKASG
                     SGKVGVTGFCMGGALTLLSAVNVPEADAAVIWYGYPPLEYVDASKIRAPLMGHWATDD
                     AAFPIATVDTLEQKLRAANVKFEFFRYDAKHAFANETADAKNLPFLKYNPQAAELAWQ
                     RTMAFLVRHLR"
     gene            complement(3829..4110)
                     /locus_tag="AC16HB_10515"
     CDS             complement(3829..4110)
                     /locus_tag="AC16HB_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR5607265.1"
                     /translation="MKAIWNDAVIAESDDTVVVEGNHYFPLEAVRREYLRPSDTTTVC
                     GWKGTANYYSLEVDGKVNRDAVWYYAAPKDAARQIRGRVAFWKGVRVAS"
     gene            complement(4131..5084)
                     /locus_tag="AC16HB_10520"
     CDS             complement(4131..5084)
                     /locus_tag="AC16HB_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5607266.1"
                     /translation="MYDLNDLYFFAQVVDHKGFAPAGRALGLPKSKLSRRIALLEERL
                     GVRLIQRSTRRFAVTDIGADYYQHCKAMLVEADAAQESVELTRAEPQGVVRLSCPVAL
                     LHYQVGQMLADFMAQCPRVQVHLEATNRRVDVIAEGFDLALRVRFPPLEDSELVMKVL
                     GESRQCLVASPAFLARHGTPRVPAALDGLPSLDLGRPHRDHVWRLDGPDGASAAIDHV
                     PRLITDDLIVLRRAALTGVGIVQLPTLLVGQDTASGALVSVLPQWRPRSGIVHAVFPS
                     RRGLLPAVRELIDFLAAHYHDAEGPCVAPAASGNNARAPNV"
BASE COUNT          735 a         1831 c         1708 g          833 t
ORIGIN      
        1 caagctgttc ctgcgcggac gctgagaagg tgcgcggcgc gctggccacg gcggtgcatg
       61 cgcgcctctc gcccgcgctg ctgctcgcct tcaccgccgc gttcatcacg ctgcagccga
      121 tctccaccga cctgtaccag gccgcattgc cgtcgatcgg caccgcgttc ggcgtacccg
      181 tcgccagcgt tcaactcaca ctgaccgtgt tcatcgccac cttcggcgtc tggcaactgc
      241 tggccgggcc gttgtcggac cgcttcggcc gccatccggt cgcgctcggt ggcgtcgtaa
      301 tctatgtcgc ggccagtgcg ctctgcacgc tcgcaccgtc gatgccttgg ctgatcgccg
      361 gccgtgcgct gcaggcggtc ggcgcgtgct cctgcctggt cgcggcgcgc gcaatggtgc
      421 gcgacgagct gacgccggag cacggcgcgc gcgtgctcgc ggtgagcggc acgatcctcg
      481 gcatctgcgt cgtgatcgcg ccgatcgcgg gcggcctgct gcaggccgca ttcggctggc
      541 gcgcgacatt cggcgcgatg ctggcgctct cgtcgctgct cgcactcgct acgctgctgc
      601 ggctgcgcga aacccaccgc acgccgaacc gcgacgcgct gcgcgccggg ccgctcgcgc
      661 gcgcctacgc ggaggtcgcg cgcgggccga gctggcatgc ctacacctgg ccggcggtgt
      721 gcagctacgc cggactgttc tcgttcatct ccggcgggtc cttcgtgctg atccgcgtgc
      781 tcggcgtggc accggtctac tacggcttct gcttcagctt catcgtctcg ggctacatcg
      841 tcggcacgct catctgccgc cgcagcgtgc cgcgtcgtgg catgccgtgg acgatgcggc
      901 gcggcgggct gctgcagttc gcggccggcg gcacgctcgc cgggctcgct ctggcgggcg
      961 tgcaccatcc gctcgcgctg ctgctgccgc agttcgtgtt cctgatcggc cacgggctga
     1021 tccagccggt cgcacaggcc ggcaccatgg cgcgctttcc gcacagcgcc ggtgtggcga
     1081 ccgcggcgat ggggctggtg atgatggtcg tcgccgcggc gatcggccag tggatcggcg
     1141 ccagctacaa cggcacggtc tatccgctga cgctgacgat cgccgcatgc ggcaccgcgt
     1201 cggcgctgac gactctcacg ctcgtgaagc gctatggccg cgtcgattga cgccgtgctg
     1261 ctgctcgggc ccaccgcgtg cggcaagagc gcgctggcgc ttgaactggc gcgcacgatc
     1321 ccgctggaga tcgtgtcggt cgactcggcg caggtgtatc gcggtatgga catcggcact
     1381 gccaagccga gcgcgcaaga gcgcgcgcgc gtgccgcagc acctgatcga tgtccgcgat
     1441 ccgtcacagc cctactccgc cgccgatttc gtgcgcgacg ccacccgcgc gatcggcgaa
     1501 atcaagcagc gcggcaagct gccgctggcg gtcggcggca ccatgctgta cgcgcgcgcc
     1561 ctgagcgcag gcttgtcgaa gctgcctgcc gccgatgcgc gggtgcgcgc gcgcatcggc
     1621 gcgcacgcac gcgaactcgg ctggcccgcg ctgcatgtcg aactcgcgca cgtcgatccg
     1681 gccaccgccg cgcggctgcc gccgaacgac agccagcgca tccagcgcgc gctggagatc
     1741 ttcgagctga ccggcacgcc gatgtcggcg ctgctcgtgg cctcccacgc ggcgccgctg
     1801 cggctgcaga cgatcgcact gctgccggcc gaccgcgccg aactgcaccg gcgcatcgag
     1861 gcgcgcttcg acgcgatgct cgcggtcggc ttcctcgatg aagtgcgcgc gctgatgaac
     1921 cgcggcgacc tcgaccccga cctgccgtcg atgcgcagcg tcggttaccg gcaggcctgg
     1981 cggcacctcg cggagcggac atcattcacc gagtttcgcg cggcaagcat cgccgccaca
     2041 cgccagctcg cgaaacggca gatcacgtgg ctgcggtcga tgcacgaagc aacccggatc
     2101 gatccattcg atccgtcgac cgcgatccgg ctgatgcaac tcctgtcgac gtacgcacac
     2161 tcgcagcgcg ggtgaagcgc tgtcggcccg ggccggcggg cgcggtgacc cgcctgcccg
     2221 agccgctgcg tcagggcttg acctgcagca gcggcccctg cggcgcaggg aagttgctcg
     2281 tgttcaggac ctggattccg gcgttgtgct cgccgttgaa tggcgtcacg ttgcccgggc
     2341 aaccggcctt gaccgcgtcg gccggagagt tgaaccacag gccgaggtgg aacgtgtgaa
     2401 tcggtccggt agcgggattc aggatcgcat ccggacacgc attgttgtgc acgctcgggg
     2461 cagccgtggc gcccggtgcg acgatgaagg tctcttcgct gaaccggccg atgaatcgac
     2521 cctgcccgac accgtggacg ttggtttcga tatcaccctg ataccaggcc agtccgaagg
     2581 gcgcgttcgg cgactggatc acgaagaagt cgaagtccgt ggccggcggc atgccctcca
     2641 ggcgcacgcg catggactcg accgatcctt ctggctcgat cgtcacgcgc gcgcgtgcgt
     2701 tgggcaggca gccggcgttg accactgccg ccgagcgcac catgtcgaat ttcagttcct
     2761 cgccactgga gcgcgcgact gcgggtgatg caagagtcgc ggccgccagt gcgagggccg
     2821 cgccggacac cgtgaagcgc aaagatggcg caaacatcgt tttctccttc gatcaatttg
     2881 tggacaacgg tcggcgtgaa gtgcctgcgc ggcacccgct gtcctcatgc gccgacaccg
     2941 atcgagctac gcgaggcagg gccgaacgga tgcagatcgc ctgcgcgaac gcggtatgcg
     3001 cggacgagcc gggtcggcgt caagcgacgc cggcaaagta ggaggattcg cgcggcgcga
     3061 gatcgcgcgc gagctaccgc aagtgccgga cgaggaacgc catcgtccgc tgccacgcca
     3121 gttccgccgc ctgtgggttg tacttcagga agggcaggtt cttcgcgtcc gcggtctcgt
     3181 tcgcgaatgc gtgcttggcg tcgtagcgga agaactcgaa cttcacgttc gccgcgcgca
     3241 gcttctgctc cagcgtgtcg accgtcgcaa tcggaaatgc ggcatcgtcg gtcgcccagt
     3301 gacccatcag cggcgccctg atcttgctcg cgtcgacata ttcgagcggc ggatagccgt
     3361 accagatcac cgcggcgtcc gcctccggca cgttgaccgc cgatagcagc gtcagcgcac
     3421 cgcccatgca gaagccggtc acgccgacct tgccgctccc ggacgccttc aggtgctgga
     3481 gcgcgccgcg cacgtcctgc cccgccgcgt cgccgaagtt cagccccttc atcaggtgct
     3541 cggcctcgtt ggcctcgagc gcaacctgcc cgcgatacag gtccggcacc agcgcgcgat
     3601 acccggcggc ggcgagcttg tccgccacgc cgcggatctg atcgttcagg ccccaccatt
     3661 cctggatcac cacgatcccc gccgcgcccg cggccgccgg ctcggcgagg tatccgttga
     3721 cgctctggcc gtccggccgc ttgaagctga tcgtcttgcc catcgtctcc tccggaaatc
     3781 agggtcgcgc atcgatacgt cgcgcggaca tgcacgatac gccgccgact acgacgccac
     3841 tctcacgcct ttccagaacg ccacccggcc gcggatctgt cttgcggcgt ccttcggcgc
     3901 ggcgtagtac cagactgcgt cgcggttgac cttgccgtcg acctcgagcg agtaatagtt
     3961 cgccgtcccc ttccagccgc acacggtcgt ggtgtcgctc ggccgcaggt actcgcgccg
     4021 cacggcttcg agcggaaagt agtgattgcc ttcgacgacg acggtgtcgt cggactcggc
     4081 gatcaccgca tcgttccaga ttgccttcat cgcaactcca tctccctgcg tcagacgttc
     4141 ggcgcccgtg cgttgttccc gcttgcagcc ggcgcaacgc acggaccttc cgcgtcgtgg
     4201 tagtgggcgg caaggaagtc gatcagctcg cgcaccgcgg gcagcaggcc ccgccgcgac
     4261 ggaaacaccg cgtgcacgat gccgctgcgc ggccgccact ggggcagcac ggacaccagc
     4321 gcgccgctgg cggtgtcctg tccgaccagc agcgtcggca actgcacgat gccgaccccg
     4381 gtcagcgcag cccgccgcag cacgatcagg tcgtcggtga tgagcctggg cacgtggtcg
     4441 atcgccgcgc tcgccccgtc gggtccgtcg agccgccaga cgtgatcgcg gtgcggccgg
     4501 ccgagatcga ggctcggcag gccgtcgaga gccgcgggca ctcgcggcgt gccgtgccgt
     4561 gccaggaatg ccggactcgc gaccaggcat tgccggcttt cgccgagcac cttcatcacc
     4621 agttcactgt cctcgagcgg cgggaaccgc actcgcagcg ccagatcgaa gccctcggcg
     4681 atcacgtcga cgcgccggtt cgtggcctcc aaatgcacct gcacgcgcgg acactgcgcc
     4741 atgaagtccg ccagcatctg gccgacctgg tagtgcagca gcgcgaccgg acacgacagc
     4801 cgcaccacgc cctgcggctc ggcgcgggtc agctcgaccg actcctgcgc cgcgtcggcc
     4861 tcgaccagca tcgccttgca gtgctggtag taatcggcgc cgatgtcggt gacggcaaat
     4921 cggcgcgtgg aacgctggat caggcgcacg ccgagccgct cctcgagcag cgcgatgcgc
     4981 cggctcagct tcgatttggg cagtccgagt gcgcgcccgg cgggagcgaa gcccttgtga
     5041 tcgacgacct gcgcgaagaa gtagagatcg ttcaggtcgt acacgcgccc ggctccatcg
     5101 ttccggc
//
LOCUS       DBNHBY010000424         5573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_296803, whole genome shotgun sequence.
ACCESSION   DBNHBY010000424 DBNHBY010000000
VERSION     DBNHBY010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_296803"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC16HB_10525"
     CDS             complement(<1..500)
                     /locus_tag="AC16HB_10525"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5607267.1"
                     /translation="MRRRFACWRIALALLAASASAQAATATVDVARHGREFDVEAVAQ
                     ASASVELAWRTLTDYEHLPQFVPGIQSVRVLRRSQVDGLERVLVEQRGEFRWLFYAQP
                     VAVRMDVLHRAPTKVDARGVATPGDDAARLELFEGRYELEPVGSGVRVRYVARIVPRF
                     ALPPVIG"
     gene            complement(448..942)
                     /gene="nudB"
                     /locus_tag="AC16HB_10530"
     CDS             complement(448..942)
                     /gene="nudB"
                     /locus_tag="AC16HB_10530"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HIR5607268.1"
                     /translation="MPSGERGTDQGEHAAVKIPESILVVIYTTRLYVLLLERADHPGF
                     WQSVTGSRASMDEALAETCSREVSEETGLICVPSDFEDWRLTHRYAIYPHWRYRYAPG
                     ITHNLEHVFGLEVEARFEPVLARDEHTNSVWLPWRDAADRCFSWTNAQAIRLLANRTG
                     AARG"
     gene            complement(977..2773)
                     /gene="aspS"
                     /locus_tag="AC16HB_10535"
     CDS             complement(977..2773)
                     /gene="aspS"
                     /locus_tag="AC16HB_10535"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5607269.1"
                     /translation="MRTEYCGLVDGKFIGQTVTVFGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDHAQVFAQAEKVRNEFCLKVTGQVRKRPEGTANPNLVSGQIEVLCTQLEIL
                     NPSVAVPFQLDEEDLSESTRLTHRVLDLRRIPMQNNLKLRYRVTMAVRKFLDAHGFID
                     IETPMLTRSTPEGARDYLVPSRVHDGMFYALPQSPQLFKQLLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDIETSFLSEQEIRAIMEEMIRTVFAETMQVQLPNPFPVMTYAE
                     AMSRYGSDKPDLRVKLELIELTDAVRDVEFKVFRQAAELKDGRVAALRVPHGGEMPRS
                     EIDAYTEFVKIYGAKGLAYIKVNEKAKGRDGLQGPIVKNLHDAALGAILSRTGAQDGD
                     VVFFGADRAKIVADGMGALRTKIGHSEFGRKHGLSEAGWRPLWVIDFPMFEFDEEEQR
                     YVAAHHPFTSPKDEHLDYLETDPSKCLAKAYDMALNGWEIGGGSVRIFREDVQSKVFR
                     ALKIGPNEARAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGAESIRDVIAFPKTQR
                     AQCLLTQAPSAVDEKQLRELHIRLRNPVPVGE"
     gene            complement(2985..3629)
                     /locus_tag="AC16HB_10540"
     CDS             complement(2985..3629)
                     /locus_tag="AC16HB_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR5607270.1"
                     /translation="MKKYLITGLLIWVPLAITLLILDTIIGWMDQTLRLVPPVWRPEA
                     LFGFSIPGLGAVLAFVVVLVTGILARNIVGRSLLHWWEGFLGRIPIVRSIYASVKQVS
                     DTMLSPKGNAFRKVVLVEFPQPEQWTLAFIVGEPTEPMRSGLKGDLVTVYVPTAPNPT
                     SGYVLLMTSERVREVDISVDDALKFHVSLGVVTPGAAAIRPAALTSESPGRAAS"
     gene            complement(3703..4029)
                     /locus_tag="AC16HB_10545"
     CDS             complement(3703..4029)
                     /locus_tag="AC16HB_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5607271.1"
                     /translation="MPIYAYKCSSCGHAQDVLQKVSDAPLTMCTACGAATFAKQLTAA
                     GFQLKGSGWYATDFKNGTAKKPDATKPDAAKPDTATTESKPAEATQPAPAAAPACGAG
                     ACPTCS"
     gene            4208..>5573
                     /locus_tag="AC16HB_10550"
     CDS             4208..>5573
                     /locus_tag="AC16HB_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR5607272.1"
                     /translation="MVARKNPPAAAAHVAALNAVENSPLQKVKVAVSDIDGVLRGKYL
                     HKDKFMSAAEGGFGFCDVVFGWDINDACYDNTKLTGWHHGFPDALARLDLSTMRNVPW
                     DGGIPFFLGEFVNADGTPHPICPRQTLKRVLKRAEKLGLQPMCGLEFEWFNFRETPQS
                     WAEKKGVGPTPLTPGMFGYSLLRANENREFFNALLDDLLAFRVPIEGLHTETGPGVYE
                     AAIQFSEALEAADRAILFKTGAKEVGKRFGIMPSFMAKWNAQYPGCSGHLHQSLSDGQ
                     KNLFYDARGRHGMSKLFESYLAGQIAVAMEFAPLFWPTINSYKRLVDGFWAPVKPTWG
                     LDNRTASFRVIAGSPKSTRLETRCPGSDINPYLAVAALVAAGLHGVEKNLKLAAKPIT
                     GTNVGAENIPRAPRSLAATTEIFKQSQVARDWLGDDFVEHFALTREWEWRQWQDGVTD
                     WELKR"
BASE COUNT          942 a         1882 c         1839 g          910 t
ORIGIN      
        1 ccgatcaccg gcggcagtgc gaagcgcggc acgatgcgcg ccacgtagcg gacgcgcacg
       61 ccgctgccga cgggctccag ttcgtagcgg ccctcgaaca gctccaggcg cgctgcatcg
      121 tcgcccggcg tcgccacgcc gcgcgcatcg accttcgtcg gggcacggtg caggacatcc
      181 atccgcaccg cgaccggctg cgcgtagaac agccagcgga attcgccgcg ctgttcgacc
      241 agcacgcgct cgaggccgtc gacctgcgac cggcgcagta cacggaccga ttggatgccc
      301 ggcacgaact gcggcagatg ctcgtagtcg gtcagcgtgc gccaggccaa ctccacgctc
      361 gcgctcgcct gcgcgaccgc ctcgacatcg aactcgcgac catgccgcgc gacatcgacc
      421 gtcgccgtgg ccgcctgcgc cgacgcgcta gccgcgagca gcgccagtgc gattcgccag
      481 caagcgaatc gcctgcgcat tggtccacga aaagcagcgg tcggccgcat cgcgccacgg
      541 cagccagaca gagttggtgt gctcgtcgcg cgcaagcacc ggctcgaaac gggcctcgac
      601 ctccagaccg aatacatgct cgagattgtg ggtgataccg ggcgcgtatc tatatcgcca
      661 atgcggatag atcgcgtaac gatgggtaag ccgccagtcc tcgaagtccg acgggacgca
      721 gatgagcccc gtctcttcgc tcacctcgcg tgaacacgtc tcggcgagcg cttcatccat
      781 cgacgcccgg ctgccggtca ccgattgcca gaatccggga tggtccgcgc gctcgagcag
      841 tagcacgtac aacctcgtcg tgtagatgac gacgaggatg gattcgggga tcttgacggc
      901 cgcatgctcg ccctggtccg tgcctcgttc gccgcttggc atgccccgta cgcccgaaca
      961 cgcaacgaac gacggcctac tcgcccaccg gcaccggatt gcgcagccgg atgtgcagtt
     1021 cgcgcagctg cttctcgtcg accgcgctcg gcgcctgggt cagcaggcat tgcgcgcgct
     1081 gtgtcttcgg gaacgcaatc acgtcgcgga tcgactcggc accggccatc atcgtgacga
     1141 tgcggtcgag tccgaacgcg atgccgccgt gcggcggcgc gccgtactga agcgcatcga
     1201 gcaggaagcc gaacttggcg cgcgcctcgt tgggcccgat cttcagcgcc cggaacacct
     1261 tgctctgcac gtcctcgcgg aagatccgca ccgaaccgcc gccgatctcc cagccgttga
     1321 gcgccatgtc gtacgccttg gcaaggcact tgctgggatc ggtctccagg tagtcgaggt
     1381 gctcgtcctt gggactcgtg aacggatgat gcgccgcgac gtagcgttgc tcttcctcgt
     1441 cgaactcgaa catcgggaag tcgatcaccc acagcgggcg ccagccggcc tcggacaggc
     1501 cgtgtttcct gccgaactcg ctgtggccga tcttcgtgcg cagcgcgccc atgccgtcgg
     1561 cgacgatctt ggcgcggtcg gcgccgaaga agaccacgtc gccatcctgc gcgccggtgc
     1621 gcgacaggat cgcgccgagc gcggcgtcgt ggaggttctt cacgatcggc ccctgcagcc
     1681 cgtcgcggcc cttcgccttc tcgttgacct tgatataggc aagccccttg gcaccgtaga
     1741 tcttcacgaa ctcggtatac gcatcgatct cggagcgcgg catctcgccg ccatgcggca
     1801 cgcgcagcgc cgcgacccgg ccgtccttca gttccgccgc ctgacggaac accttgaact
     1861 cgacgtcgcg caccgcgtcg gtcagctcga tgagttcgag tttcacgcgc aggtcgggct
     1921 tgtccgagcc gtaccggctc atcgcctcgg cgtaggtcat caccgggaac gggttcggca
     1981 gctggacctg catcgtctcg gcgaacaccg tgcggatcat ctcctccatg atcgcgcgga
     2041 tctcctgctc ggacaggaac gacgtctcga tatcgatctg cgtgaactcg ggttgccggt
     2101 cggcccgcag gtcctcgtcg cggaagcact tgacgatctg gtagtagcgg tcgaagccgg
     2161 caaccatcag cagttgcttg aagagctgcg gcgactgcgg caacgcgtag aacatgccgt
     2221 cgtgcacgcg cgacggcacc aggtagtcgc gcgcgccctc gggcgtgctc cgggtgagca
     2281 tcggcgtctc gatgtcgatg aagccgtgcg cgtcgaggaa cttgcgcacc gccatcgtca
     2341 cgcggtaacg cagcttcagg ttgttctgca tcgggatgcg gcgcaaatcg agcacgcgat
     2401 gcgtcaggcg cgtcgactcg gacaggtcct cctcgtcgag ctggaacggc accgcgaccg
     2461 acgggttcag gatttcgagc tgggtgcaca gcacctcgat ctggccggag acgaggttgg
     2521 ggttggccgt gccctcgggc cgcttgcgca cctggcccgt gaccttcagg cagaactcgt
     2581 tgcggacctt ctccgcctgg gcaaagacct gcgcgtgatc gggatcgcac acgacctgca
     2641 ccaggccttc gcggtcgcgc aggtcgatga agataacgcc accgtggtcg cggcgccggt
     2701 gcacccagcc gaagacggtg acggtctggc cgatgaactt gccgtccacg aggccgcagt
     2761 attcggtacg cattggaact ccaggcaatt gttctgtggg agcggtcgcg cggcgaccgc
     2821 gattatccga ccgtgaaatc cgaacgagga aacccgtccg gctagcggtt tcgctcgttg
     2881 tggcgcgtgg acaggacgac agacgacacg cgcggggcgc gcgcagcggt cgtggtgagg
     2941 tctgtcgtca tgttcagaac cttaacaggg ttggcgtcat ccgctcaact cgcggcgcgg
     3001 ccgggcgatt cgctggtcag cgccgccggc cggattgccg ccgcccccgg agttaccacg
     3061 ccgagcgaga catggaactt cagcgcgtcg tcgacgctga tgtcgacctc gcgcacgcgc
     3121 tcgctcgtca tcagcagcac gtagcctgag gtcgggttcg gcgcggtcgg cacgtagacc
     3181 gtgacgaggt cgcccttcag gccgctacgc atcggctcgg tcggctcgcc gacgatgaag
     3241 gccagcgtcc actgctccgg ctgcggaaat tcgaccagca cgaccttgcg aaacgcgttg
     3301 cccttgggcg acagcatcgt gtccgacacc tgcttcacgc tggcgtagat cgagcgcacg
     3361 atcggaatgc ggccgagaaa gccctcccac cagtgcagca ggctgcggcc gacgatgttg
     3421 cgcgcgagga tgccggtgac gagaacgacc acgaacgcga gcaccgcgcc gagaccgggg
     3481 atgctgaacc cgaacagcgc ctccggccgc cacaccggcg gcaccaggcg cagcgtctgg
     3541 tccatccagc cgatgatcgt gtcgagaatc agcagcgtga tcgcgagcgg gacccagatc
     3601 agcaggccgg tgatcaggta cttcttcatc tcgtttcagt gcccgcggtc cgaaacaaaa
     3661 agccgcggca ggtgccgcgg ccttcggcgc tgcaggttcc ggtcaggaac atgtggggca
     3721 tgcacctgcg ccgcaagcgg gcgcggcggc cggtgccggc tgggtcgctt cggccggttt
     3781 gctctcggtc gtcgcggtgt ccggcttcgc ggcatccggc ttggtcgcgt ccggcttctt
     3841 cgctgtgccg ttcttgaaat ccgtcgcgta ccagccactg cccttgagct ggaagcctgc
     3901 cgcggtcagt tgcttggcga acgtggccgc accacaggct gtgcacatcg tcagcggcgc
     3961 atcggaaacc ttctgcagga catcctgcgc gtgcccgcag gacgaacatt tgtaggcgta
     4021 gatcggcatg gcacccccgc gattcgtaac tggctgaatt ataaggaccg gcggcccggt
     4081 ctgagcgaga aattggggcc gcctcccggc gcttcaagcc cgtccgtcgc ccgcgcgccg
     4141 cacttggaca tcgcggcggg gctgctgcaa actgccgcgc tcataagtcg ggagacagga
     4201 gcacggcatg gtcgccagga agaacccgcc ggctgcggcg gcgcacgtcg cggcgctgaa
     4261 cgcggtcgag aactcgcccc tgcagaaggt caaggtcgcg gtcagcgaca tcgacggcgt
     4321 gctgcgcggc aagtacctgc acaaggacaa gttcatgtcg gccgccgagg gcggcttcgg
     4381 cttctgcgac gtcgtgttcg gttgggacat caacgacgcc tgctacgaca acaccaagct
     4441 cactggctgg caccacggct ttcccgacgc gctggcgcgg ctcgaccttt caaccatgcg
     4501 caacgtgccg tgggacggcg gcatcccgtt cttcctcggc gagttcgtca acgcggacgg
     4561 cacgccgcac ccgatctgtc cgcggcagac gctcaagcgc gtgctcaagc gcgccgagaa
     4621 gctcggcctg cagccgatgt gcgggctgga gttcgagtgg ttcaacttcc gcgagacacc
     4681 gcaaagctgg gccgagaaga aaggcgtcgg gccgacaccg ctcacgcccg gcatgttcgg
     4741 ctactcgctg ctgcgcgcga acgagaaccg cgagttcttc aacgcgctgc tcgacgacct
     4801 gctggcgttc cgcgtgccga tcgagggtct gcacaccgag accggcccgg gcgtgtacga
     4861 agcggcgatc cagttctccg aggcactcga ggcggccgac cgcgcgatcc tgttcaagac
     4921 cggcgccaag gaggtcggca agcgcttcgg catcatgccg agcttcatgg ccaagtggaa
     4981 cgcgcagtac cccggctgca gcgggcacct gcaccagtcg ctgtccgacg gccagaagaa
     5041 cctcttctac gacgccaggg gccggcacgg catgagcaag ctgttcgaga gctacctcgc
     5101 cgggcagatc gcggtagcga tggagttcgc gccgctgttc tggccgacga tcaacagcta
     5161 caagcggctg gtcgacggct tctgggcgcc ggtgaagccg acctggggcc tcgacaaccg
     5221 caccgcgagc ttccgcgtga tcgcgggttc gcccaagagc acgcggctgg agacgcgttg
     5281 cccgggctcc gacatcaatc cgtacctcgc ggtcgcggcg ctggtcgcag ccggactgca
     5341 cggcgtcgag aaaaacctga agctcgccgc gaagccgatc accggcacca acgtcggcgc
     5401 cgagaacatc ccgcgcgcgc cgcgttcact cgccgcgacg accgagatct tcaagcagag
     5461 ccaggtcgcg cgcgactggc tgggcgacga tttcgtcgag cacttcgcgc tgacgcgcga
     5521 gtgggagtgg cggcagtggc aggatggggt gacggactgg gagttgaagc ggt
//
LOCUS       DBNHBY010000425         9833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_364878, whole genome shotgun sequence.
ACCESSION   DBNHBY010000425 DBNHBY010000000
VERSION     DBNHBY010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_364878"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16HB_10555"
     CDS             <1..384
                     /locus_tag="AC16HB_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607273.1"
                     /translation="ADGWMGHAIEAARRREDLNASVRELSSEVGQRNATAAALDRAER
                     LVHAHDRFAATATAGSKTGSADQAAERRSHDLPPPRAIAQTMRGPSRPGFYVFRKMPS
                     LPGTKISCAAAATAAKVQPMSVLAV"
     gene            complement(415..1026)
                     /locus_tag="AC16HB_10560"
     CDS             complement(415..1026)
                     /locus_tag="AC16HB_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HIR5607274.1"
                     /translation="MQLTDILAQMGGLQSMAQELGVSESQAATGAEALIPAILGGLKK
                     QAQAQPTGLEGLGGLLGQLGGGALLDEVLAPQPTNVERGNDVLGQIFGTKDVSRAVAQ
                     NAASQTGIDPSLLKKMLPMLAMLVAGYMAKQQGAGAAAPAAPSGGGLGGGLGGLLGGL
                     FGGQAAGSAVPGGGASGLASMLDMNRDGNPLDDILQMIGKVAR"
     gene            complement(1066..1467)
                     /locus_tag="AC16HB_10565"
     CDS             complement(1066..1467)
                     /locus_tag="AC16HB_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HIR5607275.1"
                     /translation="MGFFSKILEKLGIGGAVAAPPATTPTSAAPAAPAAAPAAAPAPT
                     PIAVVDVVAQLEKRAAANPQKLNWRTSIVDLLKLLDIDSSFAARKELATELGCPADLM
                     GDSAKMNMWLHKTVLARIAANGGNVPKELLD"
     gene            complement(1701..3929)
                     /locus_tag="AC16HB_10570"
     CDS             complement(1701..3929)
                     /locus_tag="AC16HB_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR5607276.1"
                     /translation="MSAITPPAAKPLTSYRPYWAKRFGSARFLPMSRAEMEALGWDSC
                     DIVIVTGDAYVDHPSFGMAVIGRVLEAQGFRVGIIAQPDWTSAEAFAVLGRPNLFFGV
                     TAGNMDSMINGYTADRKIRSDDAYTPGGIGGKRPDRSSLVYAQRCREAFADVPIVLGG
                     IEASLRRIAHYDYWQDKVRRSILVDAKADLLLYGNAERAIVEIAHRLARREPIASVTD
                     VRGTAFIRRDGDPTAQGWFEVDSREIDTPGRVDEHISPYQERSARSECAERPITLVRA
                     ASGKLNLPPRERTVIRLPSYEQVKGDPVLYAHANRVLHQETNPGNARALVQAHGDRDL
                     WLNPPPIPLTTAEMDTVFDLPYARAPHPSYADEHGSHDGRTKIPAWEMIRFSVNIMRG
                     CFGGCTFCSITEHEGRIIQSRSEDSVIREIEAIRDHVKGFTGVISDLGGPTANMYRIA
                     CKSRAIEAACRKPSCVYPGICPNLNTDHSALIHLYRRARAVPGVKKVLIGSGVRYDLA
                     NESPAYVRELAMHHVGGYLKIAPEHTEAGPLSKMMKPGIGAYDRFKQLFDAASQAAGK
                     EQYLIPYFIAAHPGTTDEDMMNLALWLKKNGFRADQVQTFYPSPMATATAMYHSGRNP
                     LHKIARNSERVDVVRGERRRRLHKAFLRYHDPNNWPLLREALRAMGRTDLIGNGKHHL
                     VPTWQPKTDGGYHSARRKNSTPASARTSAVTKGRILTQHTGLPPGMGRQSTARPAPRK
                     PR"
     gene            3978..4757
                     /locus_tag="AC16HB_10575"
     CDS             3978..4757
                     /locus_tag="AC16HB_10575"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR5607277.1"
                     /translation="MKLTDSPDADLQRRFGGVARLYGGAAYDRFALAHAVVIGIGGVG
                     SWTAEALARSGVGALTLIDLDHIAESNTNRQIHALADEFGKAKVLAMAARIAAINPAC
                     RVTTIEEFITPENAATALPAADVVIDCIDQVSAKAALIAHCRQQRIAVVTCGAAGGRT
                     DPTRIRQDDLARARGDPLLSKVRYRLRRQHGFPREGERRAPKFGVVAVFSDESVRVPA
                     EDCDPSARLACAGYGSCVTVTAPLGFAAAAAALQWLAQRTD"
     gene            4842..5276
                     /locus_tag="AC16HB_10580"
     CDS             4842..5276
                     /locus_tag="AC16HB_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5607278.1"
                     /translation="MFKKILLPTDGSELSSRAIAGAIDMAKKLGAKIVGMTVVEPYSY
                     SSLSEYRPESYEDYEARMNKVAQERLDALATAGKAAGVEVETKVARSFSPYEAIIEAA
                     QKNDCDAIFMASHGRRGLSAVLLGSETQKVLTHSTIPVLVYR"
     gene            5372..6637
                     /gene="otnK"
                     /locus_tag="AC16HB_10585"
     CDS             5372..6637
                     /gene="otnK"
                     /locus_tag="AC16HB_10585"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HIR5607279.1"
                     /translation="MLLGCIADDLTGATDLAVNLRREGLAVVQVNGVPRPGLALPAVD
                     AVVIALKSRTIPAVEACALALDSLAWLRAQRAERIYFKYCSTFDSTPAGNIGPVTDAL
                     ADALGATIVPATPAYPRNARTVYLGHLFVGDVLLSESSMRHHPLTPMTDPNLVRVLQA
                     QTARKVGLVPLAAVDGGANAIHGRLQSLREQGCRHAVLDATFDAHLLVAGQATRELPL
                     ATGGAGLAMGIASALRSGARGSGEVTARTARGPAVILAGSCSQATLGQIAQVRERLPF
                     RQLSVEALAAGADELAGALAWARAHLGALPIVIAASVPPDQLARTQAALGRERAATLV
                     EDAFRHLARDLAAAGVRRFVVAGGETSGAVVEALADVLGFQALAVGTEIAPGVPWMTS
                     VDGEAVALALKSGNFGGPDFFMRAFEVAP"
     gene            6648..7280
                     /gene="otnC"
                     /locus_tag="AC16HB_10590"
     CDS             6648..7280
                     /gene="otnC"
                     /locus_tag="AC16HB_10590"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HIR5607280.1"
                     /translation="MSEAKLREQIARAARSMYERGLTHGSTGNISVKLDAGWLMTPTG
                     SSFGELDPARLSKLAPDGRLLSGDAPTKESFLHRAMYEQRPLSGAVVHLHATHSVAVS
                     VLDDVNADDVLPPLTAYYVMRVGRLPLVPYFAPGDEQLAHAVRARADRHHALLLANHG
                     PVVAGSTLAAAVDAIEELEATAKLFLLLRHEKIRALGPAQVAELRARFPM"
     gene            7388..7804
                     /locus_tag="AC16HB_10595"
     CDS             7388..7804
                     /locus_tag="AC16HB_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607281.1"
                     /translation="MKQVLAAAIFAATIAPAAHATDVGVSIGISQPGVYGRIDIGRFP
                     QPTVIVPQPVIIAPPPVAVVQPQPVYMWVPPGHRKKWAKHCYKYNACGVPVYFVRDDW
                     YDQNVRYGDARRGRGDDDQGSGRSKGHGKGHGKGRD"
     gene            complement(7811..8260)
                     /locus_tag="AC16HB_10600"
     CDS             complement(7811..8260)
                     /locus_tag="AC16HB_10600"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5607282.1"
                     /translation="MTCYYKRPDLTAQAIRDGWLFSGDMGYVDADGYLHLVDRKKDMI
                     DSGGVKVYPKDVEEVIARHAAVREVAVFGVPDEKWGETPVAAVILHQRGAIGEDELRE
                     WINARVAARYQRVSRVLIMDEFPRSAAGKTLKREMRAPFWEGRERKV"
     gene            complement(8285..9346)
                     /locus_tag="AC16HB_10605"
     CDS             complement(8285..9346)
                     /locus_tag="AC16HB_10605"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5607283.1"
                     /translation="MLQLATLIDRHARFRGGCPALTCEGETHSYAQFAARVNRTAQAL
                     ARLDVGRGDKVATVLANCRELVEILWAVPSLGAANVPLSPLLMGPGLLSLLKDADAAA
                     LVTQSSLLPVIESIRAELPPRLRERILLVDGSAPGCLELAPLRAAAPDAAPGVRVAPD
                     ELFNIMYTSGTTGLPKGIMHTHFIRSMYGLILGAAWRMTPESVVLHTGAIVFNGAYVT
                     LMPCFHLGAHYVLARQFDAAQMIELVERQQVTHTMMVPAQIVAMLDAPNFSPQKLASL
                     QMILSLGAPLHEEQKDKLNALLPGRFYELYGLTEGFVTVLDKTDAVRKTGSVGIPAPF
                     NEMRIVREDGTDAAVDEVG"
     gene            9479..>9833
                     /locus_tag="AC16HB_10610"
     CDS             9479..>9833
                     /locus_tag="AC16HB_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HIR5607284.1"
                     /translation="MAAVSKQFRHTDILVGFKPIDDLHREFQAILDALNDPAEAEYGE
                     DLLALHEHLLRHCATEEQFMLQENYPHYQRHKRAHEHLLESVAEARRRFDANDLEGVH
                     RFSTELISWFAIHAQS"
BASE COUNT         1481 a         3325 c         3381 g         1646 t
ORIGIN      
        1 gcagacggct ggatgggcca cgccatcgaa gccgcacgcc gccgagaaga cctgaacgcc
       61 agcgttcgcg agctgtcgtc ggaggtcgga cagcgcaacg ccaccgccgc tgcattggac
      121 cgagccgagc ggcttgtaca cgctcacgac cggttcgctg ccaccgccac cgcaggcagc
      181 aagacaggca gcgcagatca ggccgctgaa cgtcgttcgc atgatttgcc gcccccgcgc
      241 gcgatcgccc aaaccatgcg aggtccgtcg cggcccggct tctacgtctt ccgcaagatg
      301 ccgagcttgc ccggaacgaa gatcagctgc gccgcagcgg caaccgcggc gaaggtccag
      361 cccatgtccg tcctcgccgt ctgagtcgag cgctcggtgc ggcgcgcgcg gcggtcaacg
      421 tgccaccttg ccgatcatct gcaggatgtc gtccagcgga ttgccgtcgc ggttcatatc
      481 gagcatcgac gcgagtcccg acgcgccgcc gccgggtacc gcgctgccgg ccgcctgacc
      541 gccgaacagc ccgcccagca gaccaccgag cccaccgccg agcccgccgc ccgatggtgc
      601 cgccggggcc gctgcgcccg caccctgctg cttcgccatg tatcccgcca ccagcatcgc
      661 cagcatcggc agcatcttct tgagcaggct cggatcgatt ccggtctgcg acgccgcgtt
      721 ctgcgcgact gcgcggctca cgtccttggt gccgaagatc tgccccagca cgtcgttgcc
      781 gcgctccacg ttcgtcggct gcggcgcgag aacctcgtcg agcagggcgc cgccgccgag
      841 ttggccaagc aggccgccga gcccttcgag tccggtgggc tgcgcctgcg cctgcttctt
      901 caaaccgccg aggatcgccg ggatcagcgc ctcggcgccg gtcgccgcct gcgactcact
      961 gacgccgagt tcctgcgcca tcgactgcag tccgcccatc tgggcgagga tgtcggtcaa
     1021 ttgcatgttc gtgcctcctt gcgttcgtga ttcaatggcc cccgctcagt ccaggagttc
     1081 cttcggcaca ttgccgccgt ttgcggcgat gcgcgcgaga acggtcttgt gcagccacat
     1141 gttcatcttc gccgaatcgc ccatcaggtc tgccggacag cccagttccg tggccagttc
     1201 cttgcgtgcc gcaaagctgc tgtcgatgtc gagaagcttc agcagatcga cgatggacgt
     1261 gcgccagttg agcttctgcg gatttgccgc tgcgcgcttt tccagttgcg cgacgacgtc
     1321 gacgactgcg atcggtgtcg gcgcgggtgc tgccgccgga gcggcagccg gggccgcagg
     1381 cgccgcagag gttggcgttg tggcaggtgg agcggcaacc gcgccgccga tgccgagttt
     1441 ttccagaatt ttgctgaaga atcccatcac gcactccttg tgcttcggaa gggcgacgct
     1501 gccccgcccg attgcgaagg gccggccgcc attcgcgatc aatataggcg gaccttctgc
     1561 aagttcattg acccggcacg ggctcgcatg cgcatttcac cgatcgacgt gatgaaagct
     1621 tgcaggatgc gctacggcag cggggccgat cacgcgcccg gacctttcgg caaccgtctc
     1681 gcgtcgagag gcacgcactc ctaacgaggc ttccgcggtg ccggtcttgc cgtcgattgc
     1741 cggcccatcc ccggcggcaa cccggtgtgc tgcgtcagga tccttccctt cgtaacggcc
     1801 gacgtccttg cgctcgcggg cgtcgaattc ttgcgccgcg cgctgtggta gccgccgtcg
     1861 gtcttcggct gccacgtcgg cacgaggtgg tgcttgccgt tgccgatgag gtcggtgcgg
     1921 cccatcgcgc gcagcgcctc gcgcagcagc ggccagttgt tcgggtcgtg gtagcgcagg
     1981 aacgccttgt gtagccgacg gcggcgctcg ccgcgcacga cgtcgacccg ttcgctgttg
     2041 cgcgcgatct tgtgcagcgg gttgcggccc gagtgataca tcgcggtcgc ggtggccatc
     2101 ggcgacgggt agaacgtctg cacctggtcg gcgcggaagc cgttcttctt cagccaaagc
     2161 gcgaggttca tcatgtcctc gtcggtggtt cccgggtgcg cggcaatgaa gtacgggatc
     2221 aggtattgct ccttgccggc ggcctggctc gcggcgtcga acaactgctt gaaccggtcg
     2281 taggcgccga tgccgggctt catcattttc gacagcggcc ctgcctcggt gtgctcgggc
     2341 gcgatcttca ggtacccgcc cacgtgatgc atggccagtt cgcgcacgta cgcgggcgat
     2401 tcgttggcga ggtcgtagcg cacgccggag ccgatcaaga ccttcttcac gccgggcacc
     2461 gcacgcgcgc gccggtacaa gtggatcagc gccgagtgat cggtgttcag gttcggacag
     2521 atgccggggt agacgcaact cggcttgcgg cacgcggcct cgatcgcgcg cgacttgcac
     2581 gcgatgcggt acatgttcgc ggtcgggccg ccgaggtcgg agatgacgcc ggtgaagccc
     2641 ttcacatggt cgcggatcgc ctctatctcg cggatcaccg aatcctccga gcggctctgg
     2701 atgatgcggc cctcgtgctc ggtgatcgag cagaaggtgc agccgccgaa acagccgcgc
     2761 atgatgttca cggagaagcg gatcatctcc cacgcgggga tcttcgttct gccgtcgtgg
     2821 ctgccatgtt cgtccgcgta gctcggatgc ggcgcgcgcg cgtacggcag gtcgaacacg
     2881 gtgtccatct ccgcggtggt gagcgggatc ggcggcggat tcagccacag gtcgcggtcg
     2941 ccgtgcgcct gcaccagtgc gcgcgcgttg ccggggttgg tctcctggtg cagcacgcgg
     3001 ttggcatgcg cgtacagcac cggatcaccc ttcacctgtt cgtacgacgg caggcggatc
     3061 acggtccgct cgcgcggcgg caggttgagc ttgccgctcg cggcgcgcac cagcgtgatc
     3121 ggtcgttcgg cgcactcgct gcgtgccgag cgttcctggt aggggctgat gtgttcgtcg
     3181 acgcgccccg gcgtgtcgat ctctctggaa tcgacctcga accagccttg cgctgttgga
     3241 tcgccgtcgc gacgtatgaa cgcggtgccg cgcacgtcgg tgaccgacgc gatcggctcg
     3301 cgtctggcga gccggtgcgc gatctcgacg atggcccgct cggcgttgcc gtagagcagg
     3361 aggtccgcct tggcgtcgac caggatcgat cgccgcacct tgtcctgcca gtagtcgtag
     3421 tgcgcaatcc gccgcagcga cgcctcgatg ccgccgagta cgataggtac gtcggcgaac
     3481 gcctcgcggc agcgctgcgc gtacaccaga gaactgcggt cgggccgctt gccgccgatg
     3541 cctcccggcg tgtacgcgtc gtcgctgcgg atcttccggt ccgcggtata gccgttgatc
     3601 atcgaatcca tgttgccggc ggtgacgccg aagaacaggt tcggccgccc gagcacggcg
     3661 aacgcctcgg cgctcgtcca gtccggctgc gcgatgatgc cgacgcggaa cccctgcgcc
     3721 tcgaggacgc ggccgatcac cgccatgccg aagctcggat gatcgacgta cgcgtcgccg
     3781 gtgacgatca cgatgtcgca gctgtcccag ccgagcgcct ccatctccgc gcgcgacatc
     3841 ggcaggaagc gcgcgctgcc gaagcgcttg gcccagtagg ggcgatagct cgtcagcggc
     3901 ttggcggcgg gcggggtgat ggcggacata gaggacgaat tatccgcgcg acgtgcagcg
     3961 ctcaatacaa tgaccccatg aagctcaccg actcacccga tgccgacctg cagcgccgtt
     4021 tcggcggcgt ggcgcggctg tacggcggcg cggcgtacga tcggtttgca cttgcgcacg
     4081 cagtggtgat cggcatcggc ggcgtcggct cgtggacggc ggaggcactg gcgcgcagcg
     4141 gtgtgggcgc gttgacgctg atcgatctcg atcacatcgc cgagagcaac accaaccggc
     4201 agattcacgc gctcgccgat gaattcggca aggccaaggt gctggcgatg gcggcgcgca
     4261 ttgcggcgat caatccagcc tgccgcgtga cgacgatcga ggagttcatc acgcccgaga
     4321 atgccgccac cgcgctgccg gccgctgacg tcgtgatcga ctgtatcgac caggtgtcgg
     4381 ccaaggcggc gctgatcgcg cattgccggc aacagcgcat cgcggtcgtg acctgcggcg
     4441 cggcgggcgg gcgcaccgat ccgacgcgga tcaggcagga cgatctggcg cgcgcgcgcg
     4501 gcgatccgct gctgtcgaag gtgcgctatc ggctgcgccg ccagcatggg tttccgcgcg
     4561 aaggcgagcg acgcgcgccg aagttcggcg tggtcgcagt attctccgac gaatcggtgc
     4621 gcgtgccggc cgaggattgc gatccgtccg cgcgcctggc ctgtgccggc tacgggtcgt
     4681 gcgtcacggt gactgcgccg ctcggcttcg ccgcggccgc ggcggcgctg cagtggctgg
     4741 cgcaacgcac ggattagcga tggtcgtggc cgttgatcgc agtcaacaat cggccgccgg
     4801 gccgggccta ggatgaattc gaccgctaca gggagtgttg aatgttcaag aagatcctgc
     4861 tgccgaccga cggttccgaa ctgtcgtcac gcgcgatcgc gggcgcgatc gacatggcga
     4921 agaagctcgg cgccaagatc gtcggcatga cggtggtcga gccgtactcc tattcgtcgc
     4981 tgtcggaata ccggccggag tcgtatgagg actacgaggc gcggatgaac aaggtcgcgc
     5041 aggaacggtt ggatgcgctg gcgacggcag gcaaggcggc gggggtggag gtcgagacca
     5101 aggtcgcacg ctcgttttcg ccctacgagg cgatcatcga ggcggcgcag aagaacgact
     5161 gcgacgcgat cttcatggcg tcgcacggcc ggcgcggcct gagcgccgtt ctacttggga
     5221 gtgaaacgca gaaggtactg acgcacagca cgattccggt tctggtgtat cgctgataca
     5281 ccatctcgag cgggcatccg tgcccgccac ggaggggcca gacaccgcaa cggtgctggc
     5341 ccttcctttt tccgtggcac actgacgcgc catgctgctg ggctgcatcg ccgatgatct
     5401 gaccggggcg accgacctcg cggtcaacct gcggcgcgag gggctcgcgg tcgtgcaggt
     5461 caacggcgtg ccgcgcccgg ggctcgcgct gccggccgtc gatgcggtgg tcattgcgct
     5521 caagtcgcgc accatcccgg ccgtcgaggc gtgcgcgctg gcgctcgatt cgctcgcctg
     5581 gctgcgcgcg cagcgcgcgg agcgcatcta cttcaagtac tgctcgacgt tcgattccac
     5641 gccggccggc aacatcggtc cggtcacgga cgcgctggcg gatgcgttgg gcgcgacgat
     5701 cgtgccggcc acgcccgcgt atccgcgcaa cgcgcgcacc gtgtacctcg ggcatctgtt
     5761 cgtcggcgac gtgctgctgt cggagtcgag catgcgccac catccgctca cgccgatgac
     5821 cgacccgaac ctcgtgcgcg tgctgcaggc gcagacggcg cgcaaggtcg ggctggtgcc
     5881 acttgctgcg gtcgatggcg gagcaaatgc gatccatggc cggctgcagt cgctgcgcga
     5941 gcagggctgc cgtcacgcgg tactcgacgc gaccttcgat gcgcatctgc tcgtcgcggg
     6001 acaggcgacg cgcgagctgc cgctcgccac cggcggcgcg gggctggcga tgggcatcgc
     6061 ctccgcgttg cgttcgggcg cgcgcggcag cggcgaagtg acggcgcgca cggcgcgcgg
     6121 gccggcggtg atcctcgccg gcagctgctc gcaggccacg ctcgggcaga tcgcgcaggt
     6181 gcgcgagcgg ctgccgttcc gtcagctttc ggtcgaagcg ttagcggctg gcgcggatga
     6241 actcgccggc gcgctcgcct gggcgcgcgc gcacctgggc gcgctgccga tcgtgatcgc
     6301 ggcgtcggtg ccgcccgatc aactcgcgcg cacccaggcc gcgctcgggc gcgaacgcgc
     6361 cgccacgctg gtcgaagacg cgtttcgcca tctcgcgcgc gatctcgctg cggcgggcgt
     6421 gcgccgcttc gtcgtcgctg gcggcgaaac gtctggcgcg gtggtcgagg cgctcgcgga
     6481 cgtgctcggt ttccaggcgc ttgcggtcgg caccgagatc gcgcccggcg tgccgtggat
     6541 gacgtcggtc gacggcgaag cggtcgcgct cgcgctgaag tcaggcaact tcggcggtcc
     6601 ggacttcttc atgcgcgcgt tcgaggtcgc gccgtgacgc gctgccaatg agcgaggcaa
     6661 agctgcgcga gcagatcgcg cgcgcggcgc gctcgatgta cgagcgcggg ctgacgcatg
     6721 gctcgaccgg caacatcagc gtgaagctcg acgccggctg gctgatgaca ccgaccggat
     6781 cgagcttcgg cgaactcgat cccgcgcggc tgtcgaagct tgcgccggat ggccgcctgc
     6841 tgtcgggcga cgcgcctacc aaggaatcgt tcctgcaccg cgcgatgtac gagcagcgtc
     6901 ccttgagcgg cgcggtcgtg cacctgcacg cgacgcattc ggtcgcggtg tcggtgctcg
     6961 atgacgtgaa cgcggacgac gtgttgccac cgctgaccgc gtactacgtg atgcgcgtcg
     7021 gtcgcctgcc gctggtgcct tacttcgcgc cgggtgacga acagcttgcg cacgctgtgc
     7081 gcgcgcgcgc cgatcgccat cacgcgctgc tgcttgcgaa ccacggcccc gtcgtggcgg
     7141 gctcgaccct tgcggcggcg gtggatgcga tcgaggaact cgaagcgacg gccaaactct
     7201 tcctgctgct gcggcacgag aagatacgcg cgctgggccc tgcgcaggtt gccgaactgc
     7261 gcgcgcgctt tccgatgtaa gcgtcggcga aacggcctag ccccgcggcg cgctcgaccg
     7321 tttggcggtc ctccgaaatg cgcactgcgc gttagtcttg gcaggcgcca cctgcaagga
     7381 acacgccatg aagcaagtgc ttgccgctgc gatcttcgcc gcgacgatcg cgcccgccgc
     7441 gcacgcgacc gatgtcggcg tctcgatcgg gatcagccag cccggggtct atggccgcat
     7501 cgatatcggc cgcttcccgc agccgaccgt gatcgtgccg cagccggtga tcatcgcgcc
     7561 gccgccggtg gccgtcgtgc agccgcagcc ggtctacatg tgggtgccgc cgggtcatcg
     7621 aaagaaatgg gccaagcact gctacaagta caacgcgtgc ggcgtgccgg tgtatttcgt
     7681 ccgcgacgac tggtacgacc agaatgtgcg ctatggcgac gcgcgccgcg gtcgtggcga
     7741 cgacgatcag ggctcaggcc ggagcaaggg ccacggcaag gggcatggca agggccgtga
     7801 ctgacgcggg tcagaccttc cgctcccgtc cctcccagaa tggcgcgcgc atctcgcgct
     7861 tgagcgtctt gccggcggcg ctgcgcggaa actcgtccat gatcagcacg cggctgaccc
     7921 gctggtagcg cgcggcgacg cgcgcgttga tccactcgcg cagttcgtct tccccgatcg
     7981 cgccacgctg gtgcaggatg accgccgcca ccggcgtctc gccccacttt tcatccggga
     8041 ctccgaacac cgccacctcg cgcacggccg cgtggcgcgc gatcacctcc tcgacgtcct
     8101 tcggatagac cttgacgccg ccggagtcga tcatgtcctt cttgcggtcc acgaggtgca
     8161 ggtagccgtc cgcgtcgaca tagcccatgt cgccgctgaa gagccagccg tcgcgaatcg
     8221 cctgtgcagt caggtcgggc cgcttgtagt agcaggtcat caggaacggc ccgcgcccgg
     8281 cgatctagcc gacctcgtcg actgccgcgt cggtgccgtc ttcgcgcacg atgcgcatct
     8341 cgttgaacgg cgccgggatg ccgaccgagc cggtcttgcg caccgcgtct gtcttgtcga
     8401 ggaccgtgac gaagccctcg gtcagcccgt acagttcgta gaagcggccc ggcagcaggg
     8461 cgttgagctt gtccttctgc tcctcgtgca gcggcgcgcc gagcgacagg atcatctgca
     8521 gcgacgcgag cttctgcggc gagaagttcg gcgcatccag catcgcgacg atctgcgccg
     8581 gcaccatcat cgtgtgcgtg acctgttggc gttcgaccag ttcgatcatc tgcgcggcgt
     8641 cgaattgccg cgccagcacg tagtgcgcac cgaggtggaa acacggcatc agcgtcacgt
     8701 aggcgccgtt gaagacgatc gcgccggtgt gcagcacgac cgattccggc gtcatgcgcc
     8761 atgcggcgcc gaggatcagg ccgtacatcg agcgaatgaa gtgcgtgtgc atgatgccct
     8821 tcggcaggcc tgtcgtgccg ctcgtgtaca tgatgttgaa gagctcgtcc ggcgccacgc
     8881 gcacgccggg tgcggcgtca ggagcggccg cgcgcagcgg ggccagctcg aggcagcccg
     8941 gcgcgctgcc gtcgaccagc aggatgcgct cgcgcaaccg cggcggcagc tcggcacgga
     9001 tgctttcgat caccggcagc agcgacgact gcgtgacgag cgcggccgcg tcggcatcct
     9061 tgagcagcga cagcagtccc ggtcccatca gcagcggcga cagaggcacg ttggccgcac
     9121 cgagcgacgg caccgcccac aaaatctcga ccaactctcg gcagttggcg agaaccgtgg
     9181 cgaccttgtc gccacggccc acatcgagac gcgccagcgc ctgcgcagtg cggttcacgc
     9241 gcgcggcgaa ctgcgcgtag ctgtgcgttt cgccttcgca ggtcagtgcc gggcagccgc
     9301 cgcgaaaacg cgcgtggcgg tcgatcagcg tggcaagttg cagcatcgga gtcacctccc
     9361 ggccgccagt ctgctgcgaa acacggaaag tgcaagaggc aagggggcgg tttgctttcg
     9421 gtcaagcgcc gcgcgcgtgg cgcgccgtac cgtcacgcat accgttccgg aggtcgccat
     9481 ggcagccgtc tccaagcagt tcaggcacac cgacatcctg gtcggcttca agccgatcga
     9541 cgacctgcat cgcgagttcc aggccattct cgatgcgctg aacgatccgg ccgaggccga
     9601 gtacggcgag gacctgctgg cgctgcacga gcacctgctg cgccactgcg cgaccgaaga
     9661 acagttcatg ctgcaggaga actatccgca ctaccagcgc cacaagcgcg cgcacgagca
     9721 cctgctcgag tcggtcgccg aggcgcgccg caggttcgat gccaacgacc tcgaaggcgt
     9781 gcaccgcttc tcgaccgagt tgatcagctg gttcgcgatc cacgcgcaga gcg
//
LOCUS       DBNHBY010000426         1551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_499531, whole genome shotgun sequence.
ACCESSION   DBNHBY010000426 DBNHBY010000000
VERSION     DBNHBY010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_499531"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..867
                     /gene="grpE"
                     /locus_tag="AC16HB_10615"
     CDS             316..867
                     /gene="grpE"
                     /locus_tag="AC16HB_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5607285.1"
                     /translation="MGAPEQDPGKGNGAANEPAAAPAALESATVSAVEQELQQALAKA
                     NENYDLYLRAKAEGENVRRRAQEDIAKAHKFAIESFAEALLPVMDSLEKALESSQSAP
                     EAMRAGVELTLKQLQAAFEKGRLTAIYPAGEKFDPHLHQAISMVPAPEGVAPNTVVSV
                     LQKGWTIADRILRPALVTVAQSS"
     gene            929..>1551
                     /locus_tag="AC16HB_10620"
     CDS             929..>1551
                     /locus_tag="AC16HB_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR5607286.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGQPKVIENSEGARTTPSIVAYME
                     DGEILCGAPAKRQAVTNPRNTLYAIKRLIGRRFEEKEVQKDIHLMPYKIVKAPNGDAW
                     VEVRGKQIAPPQVSAEVLRKMKKTAEDYLGETVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKHGKGDRKIAVFDLGGGTFDISIIE"
BASE COUNT          304 a          471 c          543 g          233 t
ORIGIN      
        1 cgcgcccgcg tgccggatcg cgccagcgca cgtcctgcac gacgctgcga tagggtccgg
       61 gcgcctcgta ttgctcgctt tgcggccacg cggcctgcgc cagcagaagc agggccagca
      121 gcgcgattgt ccggagcggg ttcatcgtgg attacgtggc ggcggatcgg tcgtcagcct
      181 agcgagggcg gcccgccatg gctgttgccg gcgcgtcacg tgcgtaacga gatttcacgg
      241 cccaggcttg aaatcgcgcc gcccggccca atgatttgac aagtatttgc tgtttttcgt
      301 tttttcggag gactcatggg ggcgccggaa caggatccgg gcaagggcaa cggtgcagcc
      361 aacgagccgg cggcggcgcc ggccgcgctc gaatcggcaa ccgtcagcgc ggtcgagcag
      421 gaactgcagc aggcactggc gaaggccaac gagaactacg acctgtatct gcgcgcgaag
      481 gccgagggcg agaacgtgcg ccgccgggcg caggaggaca tcgccaaggc gcacaagttt
      541 gcgatcgagt cgttcgccga ggcgctgctg cctgtgatgg attcgctgga aaaggcgctg
      601 gaatcgagcc aatccgcacc cgaggcgatg cgcgccggcg tcgaactgac gctgaagcaa
      661 ttgcaggcgg cgttcgagaa ggggcggctc acggcgatct acccagcggg cgaaaagttc
      721 gaccctcact tgcaccaggc gatctcgatg gtgccggcgc cggaaggcgt agcaccgaat
      781 acggtggtca gcgtgttgca gaaaggttgg acgatcgccg atcgcatcct gcggccggcg
      841 ctcgtgacgg tcgcgcagtc gtcttgaatc gcggtcggcg gccacaattt aacggtcagt
      901 cgagttccac cactgagggg aagcggtaat ggcaaagatc atcggcatcg acctgggcac
      961 gaccaattcg tgcgtggcca tcatggaagg cggccagccc aaggtcatcg agaacagcga
     1021 aggcgcccgt accacgccgt cgatcgtcgc gtacatggag gacggcgaga tcctgtgcgg
     1081 tgctccggcc aagcggcagg ccgtcaccaa cccgaggaac acgctgtatg cgatcaagcg
     1141 gctgatcggc cggcgcttcg aggagaagga ggtccagaag gacatccacc tgatgccgta
     1201 caagatcgtc aaggcgccca atggcgacgc ctgggtcgag gtgcgcggca agcagatcgc
     1261 gccgccgcag gtgtcggcgg aggtgctgcg caagatgaag aagaccgccg aggattatct
     1321 cggcgagacg gtcaccgagg cggtgatcac ggtgccggcg tacttcaacg actcgcagcg
     1381 ccaggccacc aaggacgcgg gccgcatcgc cgggctggag gtcaagcgca tcatcaacga
     1441 gccgaccgcg gccgcgctcg cattcggcct ggacaagcat ggcaagggcg accgcaagat
     1501 cgcggtgttc gacctcggcg gcggcacgtt cgacatctcg atcatcgaga t
//
LOCUS       DBNHBY010000427         3225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1718220, whole genome shotgun sequence.
ACCESSION   DBNHBY010000427 DBNHBY010000000
VERSION     DBNHBY010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1718220"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC16HB_10625"
     CDS             <1..323
                     /locus_tag="AC16HB_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HIR5607287.1"
                     /translation="RWVLHARPRWSRRFFQMDEIEVAKWLIRSFSARIRRPLSPTHVN
                     AVRWQYATPLNPLSQTFLWDAEQRLGVAGDWCGGPRVENAYLSGISLAQEVLRSGAGE
                     RHSQ"
     gene            409..2295
                     /locus_tag="AC16HB_10630"
     CDS             409..2295
                     /locus_tag="AC16HB_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607288.1"
                     /translation="MADNQFALLGQRRFAPFFATQFLGAANDNVFKYAFTLLVTYQGA
                     QYSTLDPALAVNVIAAVFILPFLLFSATAGQVADKYDKARVMRLVKLLEVGIMLAGAW
                     GFYAASFAILLTCTFMMGLHSTLFGPVKYAYLPERLAPHELIGGNGLVEMGTFVAILL
                     GTIAAGTLIDLSARGHLYAAATCVALAVAGLAVSLAIPSAPAADPKLKIDLNPLRVTW
                     DNVRVARRDRSVFLSVLGISWLWFFGAVFLTQFPQFAQKTLGGDPAVATLLLAVFSVG
                     IGLGSVMCERMSRHQVEIGLVPFGSIGMTVFTVDLFFAVQGLPFAPNQTLTQFVAHAA
                     HWRVLADLFLLAMFAGFYSVPLYALIQTRCEPTHRARIIASNNILNALFLILAAGFAA
                     LLLGGLRWTIPQLFLGTALLNAVVAVYIYTLVPEFLLRFLSWLLVSVMYRVRKQGTER
                     IPEAGPAVIVANHVSFVDALVLLAISPRPIRFVMDQSIFRVPVLSWLFRQVKAIPIAP
                     AKVDAAMMERAFARVGEELRDGNLVCIFPEGKITASGDINPFRPGITRILARDPVPVV
                     PVALQGLWGSFFSRVSGAAMSQPFRRGLLSRIGISVGVPVAPREATPENLQELVTALR
                     GDWK"
     gene            2292..>3225
                     /locus_tag="AC16HB_10635"
     CDS             2292..>3225
                     /locus_tag="AC16HB_10635"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5607289.1"
                     /translation="MKSKRRNPKRVNLALQGGGSHGAFTWGVLDALLEDGRLDFEGVT
                     GASAGAMNAVVLADGWLRGVRARRDPRDAARAALKNFWDAVGAQPSVFALASKWSGGR
                     NGLPGHPLVVWFELLSQMFSPYQLNPLNFNPLRTALEPLIDFDRLRAHPPFKLFVCAT
                     NVRTGRVRIFREHELKIEMLLASACLPFAFQAVEVDGEHFWDGGYMGNPALWPLWYAT
                     QSKDLVLVQINPLTRADLPDTAQEIVDRVSEISFNSSLIHELRSIEFVQRLLTERRID
                     PQRYKRVYLHMIGAEDAMEAYGQASKYDTAPDFLA"
BASE COUNT          458 a         1073 c         1082 g          612 t
ORIGIN      
        1 aacgctgggt gctgcacgcg cgcccgcgct ggtcgcgccg cttcttccag atggacgaga
       61 tcgaggtggc caagtggctg attcgctcgt tctccgcacg catccgccgg ccgctgagcc
      121 ccacgcatgt gaatgccgtg cgctggcagt acgccacacc gctgaatccg ctgtcgcaga
      181 cgtttctgtg ggacgccgag cagcgcctcg gcgtcgctgg cgactggtgt ggcggcccgc
      241 gcgtcgagaa cgcatacctg tcgggcatta gcctggcgca ggaagtcttg cgcagcggcg
      301 cgggcgagcg ccactcgcag tagcaaaccg cgcgaccgac tctgcgtgac gcgcggcgcg
      361 ggcggcgcgc cgctacgatc cgcgccgtga cgacttccgc ggctcttcat ggctgacaac
      421 cagttcgcgc tgctcgggca gcgccgcttc gctccgttct tcgccacgca gttcctcggc
      481 gcagccaacg acaacgtctt caagtatgcg ttcacgctgc tggttaccta ccagggcgcg
      541 cagtattcga cgctcgatcc ggcgctcgcg gtcaacgtga tcgccgcggt gttcatcctg
      601 ccctttcttc ttttctccgc gaccgcgggc caggtcgccg acaagtacga caaggcgcgc
      661 gtgatgcggc tggtcaagct gctcgaggtc ggcatcatgc tcgccggcgc gtggggcttc
      721 tatgccgcga gcttcgcgat actgctcacg tgcacgttca tgatggggct gcactcgacg
      781 ctgttcgggc cggtcaagta cgcgtacctg cccgagcgcc tggcgccgca cgagttgatc
      841 ggcggcaacg gcctggtcga aatgggtacg tttgtcgcga tcctgctcgg cacgatcgcg
      901 gctggcacgc tgatcgacct gagcgcgcgc ggccacctgt atgcggccgc gacctgcgtc
      961 gcgctcgcgg tcgcgggcct ggcggtgagc ctcgcgatcc cttcggcacc ggccgccgat
     1021 ccaaaactga agatcgacct caatccgctg cgcgtcacct gggacaacgt gcgcgtcgcg
     1081 cgccgcgacc gctcggtgtt tctttctgtg ctcggcatct cttggctgtg gttcttcggc
     1141 gcggtattcc tgacgcagtt cccgcagttc gcgcagaaga cgctcggtgg cgatcccgcg
     1201 gtggccacgc tgctgctggc ggtgttctcg gtcggcattg ggttgggctc cgtgatgtgc
     1261 gagcgcatgt cgcgtcacca ggtcgagatc gggctcgtcc cgttcggctc gatcggcatg
     1321 acggtcttca cggtcgatct cttcttcgcc gtgcagggct tgccgttcgc gccgaaccag
     1381 accctgacgc agttcgtcgc gcatgccgcg cattggcgag tgctggcgga tctctttctg
     1441 ctcgcgatgt tcgccggctt ctacagcgtg ccgctgtacg cgctgatcca gacccgctgt
     1501 gagccgacgc accgggcgcg catcatcgcg tcgaacaaca tcctcaacgc gctgttcctg
     1561 atcctggcgg ccggcttcgc ggcactgctg ctcggcggac ttcgctggac cattccgcag
     1621 ctcttcctcg gcaccgcact gctgaatgcg gtggtcgccg tttacatcta caccctggtg
     1681 cccgagttcc tgctgcgatt cctgtcgtgg ctgctggtca gcgtgatgta ccgcgtgcgc
     1741 aagcagggca ccgaacgaat tccggaggcc ggaccggcgg taatcgtcgc caatcacgtg
     1801 tcattcgtcg acgccctggt gctgttggcg atcagcccgc ggccgatccg cttcgtgatg
     1861 gaccagtcga tctttcgcgt gcccgtgctg tcgtggctgt tccggcaggt caaggcgatt
     1921 ccgatcgcgc cggccaaggt cgatgccgcg atgatggagc gcgcgttcgc gcgcgtcggc
     1981 gaggaactgc gcgacggcaa cctggtctgc atctttccgg aaggcaagat cacggcgagt
     2041 ggcgacatca acccgttccg acccggaatc acgcgcatcc tcgcgcgtga cccggtgccg
     2101 gtggtgccgg tcgcgctgca gggcctgtgg ggctctttct tctcgcgcgt gagcggcgcg
     2161 gcgatgtcgc agccgttccg ccgcgggctg ctgtcgcgga tcggcatcag cgtcggcgtc
     2221 ccggtcgcgc cgcgagaggc cacgccggag aatctgcagg aattggtcac cgcgctgcgt
     2281 ggagactgga agtgaagtcg aagcgacgga acccgaagcg cgtgaatctg gcgcttcaag
     2341 gcggcggctc gcacggcgcg ttcacctggg gtgtgctcga cgcgctgctc gaagacggcc
     2401 ggctcgactt cgaaggcgtg accggtgcca gcgcgggcgc gatgaacgcg gtcgtgctgg
     2461 ccgacggctg gctccggggc gtgcgggcga ggcgcgaccc gcgcgacgcc gcgcgcgccg
     2521 cgctgaagaa cttctgggac gccgtaggcg cacaaccgag cgtgtttgcg ctggcatcga
     2581 agtggagtgg aggacgcaac ggcctgccgg gtcatccgct cgtggtctgg tttgagctgc
     2641 tgtcgcagat gttctccccc taccagctca acccgctgaa cttcaatccg ctgcgcaccg
     2701 cgctcgagcc gctgatcgat ttcgatcgcc tgcgtgcgca cccgccgttc aagctcttcg
     2761 tctgcgcgac gaacgtccgc accggccgcg tgcgcatctt ccgcgagcac gaattgaaga
     2821 tcgagatgct gctcgcgtcg gcgtgcctgc cattcgcgtt ccaggccgtc gaggtcgacg
     2881 gcgagcactt ctgggacggt ggctacatgg gcaatcctgc gctgtggccg ctgtggtatg
     2941 cgacgcagtc gaaagacctc gtgctggtgc agatcaaccc gttgacgcgt gcggacctgc
     3001 cggacaccgc gcaggagatc gtcgaccgcg tgagcgagat cagcttcaat tcgtcgctga
     3061 tccacgagtt gcgctcgatc gagttcgtgc agcgcctgct gacggagaga cgcatcgacc
     3121 cgcagcgcta caagcgcgtc tacctgcaca tgatcggtgc ggaggacgcg atggaggcgt
     3181 acggccaggc gagcaagtac gacacggcgc cggacttcct cgccg
//
LOCUS       DBNHBY010000428         6775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_229827, whole genome shotgun sequence.
ACCESSION   DBNHBY010000428 DBNHBY010000000
VERSION     DBNHBY010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_229827"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16HB_10640"
     CDS             <1..202
                     /locus_tag="AC16HB_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607290.1"
                     /translation="GHGTLGWTMAAGTGRVLADLISGRKPEISLEGLTIALRVTEALG
                     RQDIALSTGWSAGDPGRPRQPS"
     gene            complement(199..1563)
                     /gene="gudD"
                     /locus_tag="AC16HB_10645"
     CDS             complement(199..1563)
                     /gene="gudD"
                     /locus_tag="AC16HB_10645"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0042838 - D-glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HIR5607291.1"
                     /translation="MNATPLAAARAGTPIVRSMRVVPVAGRDGMLLNLSGAHAPFFTR
                     TLLILTDSSGNTGVGEVPGGEKIRRTLEDARPLVEGRAIADHRAILAYVRKQFADRDA
                     GGRGLQTFDLRTTIHVVTAIESALLDLFGQHVGLPVAALLGDGQQRDAVEMLGYLFFV
                     GDRTRTDLPYVDPAREPDSAGDWFRVRHAAALTPQAIVRQAEAAHAKYGFDDFKLKGG
                     VLRGEDEVDAIRALHERFPRARVTLDPNGAWRLEDAVRLLRDLRGVLAYAEDPCGAED
                     GYSGREVMAEFRRATGLPTATNMVATDWRQLAHALALQSVDIPLADPHFWTMAGSVRV
                     AQTCRDWNLTWGSHSNNHFDVSLAMFTHVAAAAPGRITAIDTHWVWQDGQRLTQEPLS
                     IRGGLVQVPKKPGLGIELDMAEVDRAHALYLQHGLGARDDAIAMQYLVPGWKFDPKRP
                     CLVR"
     gene            complement(1563..2534)
                     /locus_tag="AC16HB_10650"
     CDS             complement(1563..2534)
                     /locus_tag="AC16HB_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607292.1"
                     /translation="MKTLLRALGLAGLAALGAVAPALAAYPEKSVTMVVPFPPGGSTD
                     TIARTLGTKLAASLGQPFVVENQPGATGAIGATRVKRAPADGYTILVASIGVYATNPF
                     LQKGLQYDPARDFDLLTVAVRAPNMLVVHPSVSAKNVAELIDYLKKNPDKVSFASSGA
                     GSSDHLTAALFWQRTGTSGIHVPYKGGAPAISDLIGGHAQASFQNINAVLNHIKAGKL
                     KALAVTGDKRSAVLPDVPTLAEAGVKGADVFSWQAVAAPKGLPKEARDKLHGALVKAL
                     HDPEVAKGLAEQGFEVVGNTPEQFEQFLSQELARWKGVIETGKITLD"
     gene            2695..3681
                     /locus_tag="AC16HB_10655"
     CDS             2695..3681
                     /locus_tag="AC16HB_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5607293.1"
                     /translation="MELRTLRYFVTLAEELHFGRAAHRLALTQPPLSLAIRALETELG
                     VRLFARSRRRVEITHAGSAFLGEARAILAHAADAVQLARAAERGEVGRLAIGFMSASV
                     YTLLPPVLREFAAGHPRVRLELRELTLPQQMAALRRGDIHAGFVRPPVTDAELESEVL
                     LQEPLCVALPAGHPLAALRRVRAAKLAGEPFVMFQRAPGLVLHDLVLGFCLQQGFSPR
                     VAQEAAQTHAVIGLVSAGIGVALVPASAQEIRLRGVEIRPLFERSPPVGTALAWRRDD
                     ASPVRAAFVDTARSIARRRTNGTAQVTRAGERAARRLSAARSSTGPRARRRA"
     gene            complement(3632..4768)
                     /gene="lptG"
                     /locus_tag="AC16HB_10660"
     CDS             complement(3632..4768)
                     /gene="lptG"
                     /locus_tag="AC16HB_10660"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR5607294.1"
                     /translation="MRTLRRYLTREIALATGFVLFALLALFAFFDLIAQLEDISSGYQ
                     LSTAFAYVALSLPSRTYELMPIAALIGTIYALSKLASNSEFTIMRVSGMSTRRLAATV
                     IRAALAFIALTYLFGELVAPPAERVAQRLKLAAQGGSVEQFRSGVWVRDAVTGADGQV
                     ERWRFVNVRQVRPDATLQSMRLFEFDRDYRLRVIGTAESGRFMIDAKGPAWQLNNVVA
                     TLLPVVSPTDTAQSGDRTMIVRDATRRWASELTPEIFSVLLVQPERMAALDLEQYIRH
                     LSANRQQTDRYEIALWNKVFYPLAILVMMMLALPFAYLHVREGSVSLKIFTGVMIGVT
                     FYMLNKLFAHLGLLNTWPPVVVAALPSLVMLGVALAALYWIERR"
     gene            complement(4770..5972)
                     /gene="lptF"
                     /locus_tag="AC16HB_10665"
     CDS             complement(4770..5972)
                     /gene="lptF"
                     /locus_tag="AC16HB_10665"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR5607295.1"
                     /translation="MVFQRAIVAELSNTAGAVFTVLFSIIFSVGLVRILGEAAGGRID
                     SGAVFAIVALTALTYLPTVLTLTLFVSVLMTVSRAYRDSEMVVWFASGQSLLAWIWPV
                     LRFAAPIIVLVAVLALQLSPWASRQIVESRMRFEQRDDVSKVAPGRFIESSGADRVFF
                     VESVDVAGAQVKNVFVSHRSQGREGVIVAAQGVIEVRPDGERYLVLQQGRRYEGRPGE
                     AEYRMFEFDRYAIRLDTKPEAPLAELAARAKSSDELWRQPTPWNLGELMWRVGLPIVA
                     LLVALLAIPLGYTNPRVGRSFNLILAVLAFLLYNNAMSIVQSSVQRDRLPFSVGLWLA
                     HAAVALVIALMFVRRVYWQGWVPRRLVNALKRRRAADDEPPPPSRPAPAKGERGAAQR
                     ITSDSLEG"
     gene            6096..>6775
                     /locus_tag="AC16HB_10670"
     CDS             6096..>6775
                     /locus_tag="AC16HB_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5607296.1"
                     /translation="MEFSTKVASADAVKADCVAVGIYADGALTAAARRIDAASKGAVT
                     AAVKSGDATGKRGNAVMLRNLAGVASPRVLLLGLGKSDEFTDKAFTEAVRVAVRGAGA
                     GVKDLALAVADWKTKGRDHAWQARTLAVAARETVFRTDELKSRKDDNGPRVAQILLLT
                     DARDAHAERGLKEGVAIANGAALTKRLGNLPGNVCTPTYLGEQARQLGNEWKLDVEVL
                     GRKDIEKL"
BASE COUNT         1082 a         2355 c         2364 g          974 t
ORIGIN      
        1 cggccacggc acgctcggct ggaccatggc cgccggcacc gggcgcgtgc tggcggacct
       61 gatcagcggg cggaagccgg agatcagcct cgaggggttg acaatcgcgc tacgcgtgac
      121 cgaagcgctc ggccgtcagg acatcgcgct aagcacgggc tggtccgcag gagaccctgg
      181 tcggccgcga caaccgtcct aacggaccag gcagggccgc ttcgggtcga acttccagcc
      241 cgggaccaag tactgcatcg cgatcgcgtc gtcgcgtgcg cctaggccgt gctgcaggta
      301 cagcgcgtgc gcccgatcca cttccgccat gtcgagctcg atgccgaggc cgggcttctt
      361 cggcacctgc acgagcccgc cgcggatcga cagcggctcc tgcgtcaggc gctggccgtc
      421 ctgccagacc cagtgcgtgt cgatcgcggt gatgcggccc ggggcggccg ccgcgacgtg
      481 cgtgaacatc gccagcgaca cgtcgaagtg attgttcgag tgcgagcccc aggtcaggtt
      541 ccagtcgcga caggtctgcg cgacacgcac cgagcccgcc atcgtccaga aatgcggatc
      601 ggcgagcggg atatcgaccg actgcagcgc gagcgcgtgc gccagctgcc gccagtcggt
      661 ggcgaccatg ttggtcgccg tcggcagccc ggtggcgcga cggaattccg ccatcacctc
      721 gcgcccggag tagccgtcct cggcgccgca gggatcttcc gcgtacgcga gcacgccgcg
      781 caggtcgcgc agcagccgca ccgcatcctc gagccgccag gcgccgttcg gatcgagcgt
      841 cacgcgcgcc cgcggaaagc gctcgtgcaa cgcgcggatc gcgtcgactt cgtcttcgcc
      901 gcgcagcacg ccgcccttca gcttgaagtc gtcgaagccg tacttcgcgt gcgccgcctc
      961 ggcctggcgc acgatcgcct gtggcgtgag cgccgcggcg tgccgcacgc ggaaccagtc
     1021 gccggcgctg tcgggttccc gggccggatc gacatacggc aggtcggtcc gggtacggtc
     1081 gccgacgaag aagagatagc cgagcatctc gacggcgtcg cgctgctggc cgtcgccgag
     1141 cagcgccgca accggcagcc cgacgtgctg tccgaacaga tcgagcagcg cgctttcgat
     1201 cgcggtgacg acgtgaatcg tcgtgcgcag gtcgaacgtc tgcagaccgc gcccgccggc
     1261 gtcgcggtcg gcgaactgct tgcgcacata cgcaaggatc gcccggtgat cggcaatcgc
     1321 gcggccttcg acgagcggac gcgcgtcctc cagcgtccgg cggatcttct cgccgcccgg
     1381 cacttcgccc acgccggtgt ttccgctgct gtcggtcagg atcagcagag tgcgggtgaa
     1441 gaacggcgca tgcgctcccg agagattgag cagcatgccg tcgcgccccg cgacgggcac
     1501 cacgcgcatc gagcggacga tcggcgtccc ggcccgggcg gccgcaagcg gtgtcgcgtt
     1561 catcagtcga gcgtgatctt gccggtctcg atcacgccct tccagcgcgc cagctcctgg
     1621 ctcaggaact gctcgaactg ctcgggcgtg ttgccgacga cctcgaagcc ctgctcggcg
     1681 aggcctttcg cgacctccgg atcgtgcagc gccttgacca gcgcgccgtg cagcttgtcg
     1741 cgtgcctcct tcggcagacc cttgggagca gcgaccgcct gccacgagaa cacatcggcg
     1801 cccttgacgc cggcctcggc gagcgtgggc acgtcgggca gtacggcgga gcgcttgtcg
     1861 cctgtcaccg ccagcgcctt cagcttgccg gccttgatgt gattgagcac cgcgttgatg
     1921 ttctggaacg acgcctgcgc gtggccgccg atcagatccg agatcgccgg cgcgccgccc
     1981 ttgtacggca cgtggatgcc ggaggtcccc gtgcgctgcc agaacagcgc cgcggtcaga
     2041 tggtcggacg agcccgcgcc gctggaagcg aacgacacct tgtccggatt cttcttcaga
     2101 tagtcgatca gctcggcgac gttcttcgcc gacacgctcg gatgcacgac cagcatgttg
     2161 ggcgcgcgca ccgcgactgt cagcagatcg aagtccctgg ccgggtcgta ctgcaggccc
     2221 ttctgcagga atggattggt cgcgtacacg ccgatcgacg cgacgaggat cgtgtagccg
     2281 tcggccggcg cacgcttgac gcgcgtggcg ccgatcgcgc ccgtggcgcc cggctggttc
     2341 tcgaccacga atggctggcc gaggctcgcg gcgagcttcg tgcccagcgt gcgcgcgatc
     2401 gtgtcggtcg agccgccggg cgggaacgga acgaccatgg tgaccgactt ttcgggatag
     2461 gcggcgagcg ccggggcaac cgcgcccagg gccgcgaggc cagcaaggcc aagggcgcgc
     2521 agcaatgtct tcatcgggtg tctccgggtg ggtgatcggc ggcagcgccg cgccggtgcc
     2581 gtggcggccg cgcacgcggc ttccgaacga cgctcagcgt aggtggcatc gagatgcgcg
     2641 tccaatatat atttcaatgg cgattgattt gtcctgcaaa tcaccggagc ggccatggaa
     2701 ctgcgcacgc tgcgttactt cgtcacgctg gcagaggagc tgcacttcgg ccgcgccgcg
     2761 caccggctcg cgctcacgca gccgccgctg tcgctcgcga tccgcgcgct cgagacggaa
     2821 ctcggcgtgc gcctgttcgc gcgcagccgc cgccgggtcg agatcacgca cgcgggcagc
     2881 gccttcctcg gcgaggcgcg cgcgatcctc gcgcacgcgg ccgacgcggt gcagctcgcg
     2941 cgcgccgccg agcgcggcga ggtcggccgg ctggcgatcg gcttcatgtc cgccagcgtc
     3001 tacacgctgc tgccgccggt gctgcgtgag ttcgcggccg gtcatccgcg cgtgcggctc
     3061 gaactgcgcg agctgacgct gccgcagcag atggcggcgc tgcggcgggg cgacatccat
     3121 gcgggcttcg tgcgcccgcc ggtgaccgac gccgaactcg aatcggaagt gctgctgcag
     3181 gagccgctgt gcgtggcgct gccggccggg catccgctgg ctgccctgcg ccgcgtgcgc
     3241 gcggcgaagc tggcgggcga gccgttcgtg atgttccagc gcgcgcccgg cctcgtgttg
     3301 cacgatctcg tgctcgggtt ctgcctgcag cagggcttct cgccgcgcgt cgcgcaggag
     3361 gccgcccaga cgcacgcggt gatcggcctc gtgtcggccg gcatcggggt ggcgctggta
     3421 ccggcatcgg cgcaggaaat tcggctgcgc ggcgtcgaga tccggccgtt gttcgagaga
     3481 tcgccgcccg tgggcaccgc gctggcgtgg cggcgcgacg atgcctcgcc cgtgcgcgcg
     3541 gctttcgtcg acaccgcgcg cagcattgcg cgccggcgca cgaacgggac ggctcaggtg
     3601 acgcgcgcgg gcgagcgcgc agcgcggcga ctcagcgccg ctcgatccag tacagggccg
     3661 cgagcgcgac gccgagcatg accaggctcg gcagcgcggc gaccacgacc ggcggccacg
     3721 tgttcagcag cccgagatgc gcgaacagct tgttcagcat gtagaacgtc acgccgatca
     3781 tcacgccggt gaagatcttc agactcacgc tgccctcgcg cacgtgcagg tacgcgaatg
     3841 gcagcgccag catcatcatc accaggatcg cgagcggata gaacaccttg ttccacagtg
     3901 cgatttcgta gcgatcggtc tgctggcggt tggccgaaag atggcggatg tactgctcca
     3961 gatcgagtgc cgccatgcgc tcgggctgca ccagcagcac gctgaagatc tcgggcgtga
     4021 gctccgacgc ccaccgccgc gtcgcgtcgc ggacgatcat ggtgcgatcg cccgactgcg
     4081 ccgtgtcggt cggcgagacc accgggagca gcgtcgccac gacgttgttc agctgccacg
     4141 ccgggccctt ggcatcgatc atgaaacgtc cgctttccgc ggtgccgatc acacgcagcc
     4201 gatagtcacg gtcgaactcg aacaagcgca tcgactgcag cgtggcgtcg ggccgcacct
     4261 ggcgcacgtt gacgaagcgc cagcgttcga cctgtccgtc ggcgcccgtg accgcgtcgc
     4321 gtacccacac gccggagcgg aactgctcga ctgacccgcc ctgggccgcg agcttcaggc
     4381 gctgcgccac gcgctcggcc ggcggcgcga ccagctcgcc gaacaggtac gtgagcgcaa
     4441 tgaacgccag cgccgcccgg atcacggtgg cggcgagccg gcgcgtgctc atgcccgaca
     4501 cgcgcatgat ggtgaactcg gaattcgacg ccagcttcga cagcgcgtag atggtgccga
     4561 tcagcgccgc gatcggcatc agctcgtagg tgcgcgacgg cagcgacagc gcgacgtacg
     4621 cgaacgcggt ggacagctgg tagccggagc tgatgtcctc gagctgcgcg atcaggtcga
     4681 agaacgcgaa cagcgccagc agcgcgaaca gcacgaagcc ggtcgcgagc gcgatttcac
     4741 gcgtgagata ccgacgcagc gtgcgcattt cacccctcca gcgaatcgct cgtgatccgc
     4801 tgcgcggcgc cccgctctcc ctttgcagga gcggggcggg aaggcggcgg cggttcgtca
     4861 tcggcggcgc gccgtcgctt gagcgcgttc accagccgcc gcggcaccca gccctgccag
     4921 tagacgcggc gcacgaacat cagcgcgatc accagcgcca ccgccgcatg ggcgagccag
     4981 agcccgaccg agaacggcag gcggtcgcgc tgcaccgacg actgcacgat cgacatcgcg
     5041 ttgttgtaga ggaggaatgc cagcacggcg aggatcaggt tgaacgagcg tcccacgcgc
     5101 ggattcgtgt agccgagcgg gatcgccagc agcgcgacca gaagcgccac gatcggcagt
     5161 ccgacgcgcc acatcagctc gcccaggttc catggcgtcg gctggcgcca cagttcgtcg
     5221 ctcgacttgg cgcgcgcggc cagctccgcc agcggtgcct ccggcttggt gtcgagccgg
     5281 atcgcgtagc ggtcgaactc gaacatgcgg tattcggctt cgcccggacg accttcgtag
     5341 cggcgcccct gctgcagcac cagatagcgc tcgccgtcgg gacgcacctc gatcacgccc
     5401 tgcgcggcga cgatcacgcc ttcgcgcccc tggctgcgat ggctgacgaa cacgttcttg
     5461 acctgcgctc cggcgacgtc cacgctttcg acgaagaaca cgcggtcggc gccgctcgat
     5521 tcgatgaagc ggccgggcgc gaccttgctc acgtcgtcgc gctgttcgaa gcgcatcctg
     5581 ctctcgacga tctgccggct cgcccacggc gacagctgca gcgcgagaac cgcgaccagc
     5641 acgatgatcg gagccgcgaa gcgcagcacc ggccagatcc acgccagcag actctggccg
     5701 gacgcgaacc aaaccaccat ctcggagtcg cggtacgcgc gcgacaccgt catcagcacc
     5761 gacacgaaca gcgtcagcgt cagcacggtc ggcaggtagg tgagcgcggt cagcgcgacg
     5821 atcgcgaaca ccgcgccgct gtcgatgcgg ccgccggcgg cctcgccgag gatgcgcacc
     5881 aggccgaccg agaaaatgat cgaaaacaga accgtgaaca ccgcgccggc cgtgttcgac
     5941 agctcggcga cgatggcgcg ctggaagacc atgatttatg cggatcgcgg gggaatccgt
     6001 gcaggccgtc gctatactgc gcgcccgaag caggctggcc agtccgagag cgccgctgtt
     6061 ttcgcgctca cacatccccg tccgaggaag acccgatgga atttagcacg aaggttgcat
     6121 ccgccgacgc cgtgaaagcc gactgcgtgg cggtcggcat ctacgccgac ggcgcgctga
     6181 ccgcggccgc gcgccgcatc gatgccgcgt cgaaaggcgc ggtcacggcg gcggtgaaga
     6241 gcggcgacgc gaccggcaag cgcggcaatg ccgtgatgct gcgcaacctt gcgggcgtgg
     6301 caagcccgcg cgtgctgctg ctcgggctgg gcaagagcga cgagttcacc gacaaggcgt
     6361 tcaccgaggc ggtgcgcgta gccgtgcgcg gggccggcgc cggcgtgaag gatctggcgc
     6421 tcgcggtcgc cgactggaag acgaagggcc gcgaccacgc ctggcaggcg cgcacgctcg
     6481 cggtcgcggc acgcgagacg gtgttccgca ccgacgagct gaagagcagg aaggacgaca
     6541 acggtccgcg ggtggcgcag atcctgctgc tgaccgacgc gcgcgacgcg cacgccgagc
     6601 ggggactgaa ggaaggtgtc gcgatcgcca acggcgctgc gctcaccaag cgcctgggca
     6661 acctgcccgg caacgtgtgc acgccgacct acctcggcga gcaggcgcgg caactcggca
     6721 acgaatggaa gctcgatgtc gaagtgctgg gccgcaagga catcgagaaa ctgaa
//
LOCUS       DBNHBY010000429         2132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1245034, whole genome shotgun sequence.
ACCESSION   DBNHBY010000429 DBNHBY010000000
VERSION     DBNHBY010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1245034"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="AC16HB_10675"
     CDS             <1..1314
                     /locus_tag="AC16HB_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HIR5607297.1"
                     /translation="GARFDLVRSRFSSPRRFTSASPDSMQRSPAIPFILVAVLLDVMG
                     IGLLIPVLPSLVGQLAGGREAQTYWYGLMILTFGSAQFLCAPLLGALSDHYGRRPVLL
                     AAIAGLGTMFLLTPLVNTLTQLLATRIVGGALASNFSVANAYVADISSHENRAKSFGK
                     IGAAFGVGFIVGPMLGGLLGHIDIRLPFFVAAGLSFLNFLYGWFVLPESLPQERRKRI
                     ELARANPLTSLYGLARLKSVGALVAVFALTNLAQFILHAVWVLYTAFRFSWGPRESGL
                     SLFVVGLMAAIVQGGLLGWLLRQFGERRVVVAGLASGTLAFAGYGLATQGWMMYAVIV
                     ANLLAFAVSAALNAIVSKAADAREQGLAMGSLSSLNSLMAVIAPLLGTPLLAVVSHLP
                     AQDWRIGAPFFLSAALNLAALLLALSHFARHREHAVAHVADPAAR"
     gene            1352..>2132
                     /locus_tag="AC16HB_10680"
     CDS             1352..>2132
                     /locus_tag="AC16HB_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR5607298.1"
                     /translation="MTTLDTKLNTRSDDFRAAAEHMRGLVADLKSKFALMAEGGGAEA
                     RAKHTARGKLLPRDRVQMLLDPGTPYLEIGQLAGYGMYDDAAPCGGIITGIGRVAGRE
                     CMIVCNDATVKGGTYFPITVKKHLRAQEIARENRLPCIYLVDSGGANLPNHTDVFPDR
                     EHFGRIFYNIANMSAQGIPQIACVMGSCTAGGAYVPAMSDESIIVKGQGTIFLGGPPL
                     VKAATGEVVSAEDLGGADVHARLSGVADHYAMDDGHALSLVR"
BASE COUNT          280 a          741 c          733 g          378 t
ORIGIN      
        1 ggcgcgcgat tcgacttggt acgctcgcgc ttttcgtcgc cgcgccgttt cacctcggca
       61 tctccggaca gcatgcagcg ttcgcccgcg atcccgttca tcctcgtcgc cgtgctgctc
      121 gacgtgatgg gaatcgggct gctgatcccg gtgctgccat cgctcgtggg tcagcttgcc
      181 ggcggccgcg aggcccagac ctactggtac ggcctgatga tcctgacctt cggcagcgcg
      241 cagttcctgt gcgcgccgct gctcggcgcg ctgtccgacc actacgggcg gcggccggtg
      301 ctgctcgccg cgatcgcggg gctcggcacg atgttcctgc tgacgccgct ggtgaacacg
      361 ctgacgcaac tgctggccac acgcatcgtc ggcggtgcgc tcgcgtccaa cttctcggtc
      421 gccaacgcct acgtcgccga catctcgtcg cacgagaacc gcgccaagag cttcggcaag
      481 atcggcgccg cgttcggcgt cggtttcatc gtcgggccga tgctgggcgg gttgctcggt
      541 cacatcgaca tccggctgcc gttcttcgtc gcggcgggcc tgtcgttcct gaatttcctg
      601 tacggctggt tcgtgctgcc cgaatcgctg ccgcaggagc ggcgcaagcg gatcgaactg
      661 gcgcgcgcca atccgctgac ctcgctgtac ggcctggccc ggctgaagag cgtcggcgcg
      721 ctggtcgcgg tgttcgcact gaccaacctg gcgcagttca tcctgcatgc cgtgtgggtg
      781 ctgtacacgg cgttccgttt ctcctggggg ccgcgcgaga gcgggctgtc gctcttcgtc
      841 gtcggtctga tggccgcgat cgtccagggc ggattgctcg gctggctgtt gcggcagttc
      901 ggcgagcggc gcgtcgtcgt ggccggcctg gcgtcgggca cgcttgcatt cgcgggctac
      961 ggcctcgcca cccagggttg gatgatgtac gcggtcatcg tcgccaattt gctcgcgttc
     1021 gcggtcagcg cggcgctgaa cgccatcgtg tccaaggccg ccgatgcgcg cgagcagggc
     1081 ctggcgatgg gctcgctgtc gtcgctgaac agcctgatgg cggtgatcgc gccgttgctc
     1141 ggcacgccgc tgctggccgt agtcagccac ctgccggcgc aggactggcg catcggcgcg
     1201 ccgttcttcc tgtcggcggc gctgaacctg gcggcgctgc tgcttgcgct ctcgcacttc
     1261 gcgcggcacc gcgagcatgc ggtcgcgcac gtggccgatc cggccgcgcg ctgaactacc
     1321 attcgttcgt ccgcgcgccg acgacaagac gatgaccacg ctcgacacga agctgaacac
     1381 ccgctccgac gacttccgcg ccgccgccga gcacatgcgc gggctggtcg cggacctgaa
     1441 gtcgaagttc gcgctgatgg ccgagggcgg cggcgccgag gcgcgcgcca agcacactgc
     1501 gcgcggcaag ctcttgccgc gcgaccgcgt gcagatgctg ctcgaccccg gcacgccgta
     1561 tctggagatc ggccagctcg cgggctacgg catgtacgac gacgccgcgc cgtgcggcgg
     1621 catcatcacc ggcatcggcc gcgtggcagg gcgcgagtgc atgatcgtgt gcaacgacgc
     1681 gacggtgaag ggcggcacgt acttcccgat cacggtgaag aagcacctgc gcgcgcagga
     1741 gatcgcgcgc gagaaccggc tgccctgcat ctatctcgtc gactccggcg gcgccaacct
     1801 gccgaaccac accgacgtct tcccggaccg cgagcacttc ggccgcatct tctacaacat
     1861 cgccaacatg tcggcgcaag gcatcccgca gatcgcctgc gtcatgggct cctgtacggc
     1921 gggcggtgcg tatgtgccgg cgatgtccga cgagtcgatc atcgtcaagg gacagggcac
     1981 catcttcctc ggcggtccgc cgctggtgaa ggccgcgacc ggcgaggtgg tctcggccga
     2041 ggatctcggc ggcgcggatg tgcacgcgcg cctctccggg gtcgccgacc attacgcgat
     2101 ggacgacggg cacgcgctct cgctggtgcg gc
//
LOCUS       DBNHBY010000430         3342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1718549, whole genome shotgun sequence.
ACCESSION   DBNHBY010000430 DBNHBY010000000
VERSION     DBNHBY010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1718549"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16HB_10685"
     CDS             complement(<1..230)
                     /locus_tag="AC16HB_10685"
                     /inference="COORDINATES: protein motif:HMM:NF016161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HIR5607299.1"
                     /translation="MYATLKVIHVTCVIASGAGFLARGLLMLADSPWLDARFVRVVPH
                     IVDTLLLASAITMAVIAHLAPTQQPWLMAKIVG"
     gene            complement(241..1521)
                     /locus_tag="AC16HB_10690"
     CDS             complement(241..1521)
                     /locus_tag="AC16HB_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR5607300.1"
                     /translation="MTQKTLPLTEDRFSPAAGTSLRWLLAAPHRLFFFFGMLGLGTAS
                     LWWLVQIAARTAGHPLALELPPAWMHGWLMGNAFLPFFMFGFLFTAGPKWLNVIAPSA
                     RDVLRPGALTLAAFALALIGAHASRLLVAAGALLLTLAWSLLLARFYRLILASRVADQ
                     VHARLVLAAFTLGALAHLVFAAGVAQLDLRWIHSAEMLTLWFFVAPVFVTVAHRLIPF
                     FTANVVPFLDAWRPLWLLWTFLAIVIGHGVLPVIAILASSGFVSLLRVAFDVVAAALL
                     LFVAWRWGVVQSLRNRLLAMLHLGFLWLGIALALYAWNAATLLAGLSQASLGLAPLHG
                     LTMGFFGSTMLAMVARVTSGHSGRALVADNLTWFLYWMLQAAVLARVLVDAFPSQSAW
                     LTLAAIVLWCAAILPWAARSALIYLRPRPDGRPG"
     gene            complement(1518..1964)
                     /gene="ubiT"
                     /locus_tag="AC16HB_10695"
     CDS             complement(1518..1964)
                     /gene="ubiT"
                     /locus_tag="AC16HB_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HIR5607301.1"
                     /translation="MNPTLPPLPAPPDAVRRLIERLPIEPPSFVLAKVLNRVLLPRLD
                     ADARAALSDRCVEVHVSDFGVRMRLALGARGFALAPRGAEVALHVSATASAFWRMASG
                     EEDADTLFFERALVMEGDTEFGLLVKNTLDAIGPLVPAFLRQPRAR"
     gene            complement(1961..2875)
                     /gene="ubiV"
                     /locus_tag="AC16HB_10700"
     CDS             complement(1961..2875)
                     /gene="ubiV"
                     /locus_tag="AC16HB_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiV"
                     /protein_id="HIR5607302.1"
                     /translation="MEVTVPQFELSVGPVLTYWPRRTLTEFYARVAESAAHTVYLGEI
                     VCSRRHEMKVDDWLALARDLAAAGKEVVLSGQALIESEAELRTLHRFTDNGEFLVEAN
                     DSAAALRMNGRPFVIGPHVNVYSAPALDEFAQMGAVRWVPPLELSLDAIAAVNPEDRT
                     PPVETELFAFGRMPLAFSARCFTARHYGRNRDECEFRCLDHPDGLVLKTQEGDPFLTL
                     NGLQTQSARVQCLIAWRSAVVEAGVRRLRLSPIAHNFEQVIDAFDRVFNRGADADAAL
                     AELIELGLPGAPADGYAHRHPGFEWSHA"
     gene            complement(2866..>3342)
                     /locus_tag="AC16HB_10705"
     CDS             complement(2866..>3342)
                     /locus_tag="AC16HB_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR5607303.1"
                     /translation="SSYATGQSPNNFGACSPALHVRWIEHPDHSVDARLNGIQINHFG
                     TSEPRAYPTVCKGRYAVGNDGDGEVGHAIEEPTSLNTLPILPDLMAAGVAAIKIEGRQ
                     RSPAYVEQVTRVWRAAIDACHAQPARFSPQPAWIAALGRLAEGQQHTLGAYSRPWK"
BASE COUNT          595 a         1166 c         1155 g          426 t
ORIGIN      
        1 ccgacgatct tcgccatcag ccacggctgc tgggtcggcg ccagatgcgc aatcactgcc
       61 atcgtgatcg ccgaggcgag cagcagcgtg tcgacgatgt gcggcaccac gcgcacgaag
      121 cgcgcatcca gccatggcga gtcggccagc atcagcaggc cgcgcgcgag aaagccggcg
      181 ccgctcgcga tcacgcaggt gacgtgaatt accttcagcg tcgcgtacat gatcgcgcgc
      241 tcagcccggt cggccgtcgg gacgcggccg cagatagatc agcgcactgc gcgccgccca
      301 cggcaggatg gccgcacacc acagcacgat cgcggcgagc gtcagccacg ccgactgcga
      361 cggaaacgcg tcgaccagca cccgcgcgag caccgccgcc tgcagcatcc agtacaggaa
      421 ccacgtcagg ttgtcggcaa cgagcgcgcg gccgctgtga cccgaagtga cgcgcgcaac
      481 catcgccagc atcgtgctgc cgaagaagcc catcgtcagc ccatgcagcg gggcgagtcc
      541 gagcgacgcc tgcgacagcc cggcgagcag cgtcgccgcg ttccaggcgt acagcgcgag
      601 cgcgatgccg agccacagga agcccaggtg cagcattgcg agcagccggt tgcgcaggct
      661 ctgcaccacg ccccagcgcc aggcgacgaa gagcagcagc gccgccgcca cgacgtcgaa
      721 cgccacgcgc agaagactga cgaaccccga agaggcgagg atcgcgatca cgggcagcac
      781 gccatggccg atcacgatcg cgaggaacgt ccacagcagc cacagcggcc gccacgcgtc
      841 gaggaacggc accacgttgg ccgtgaagaa cggaatcagc cggtgcgcga ccgtcacgaa
      901 caccggcgcg acgaagaacc acagcgtcag catctcggcc gaatgaatcc agcgcaggtc
      961 gagttgcgcg acgccggccg cgaacacgag gtgcgccagc gcgccgagcg tgaatgccgc
     1021 gagcaccagt cgcgcgtgga cctggtccgc gacccggctg gcgaggatca gccgatagaa
     1081 acgcgccagc agcaggctcc acgcaagcgt gagcagcagc gcgccggcgg ccaccagcag
     1141 ccggctcgcg tgcgcaccga tcagcgcgag cgcaaacgcg gcgagcgtca gcgcgccggg
     1201 ccgcagcacg tcgcgcgccg acggcgcgat cacgttcagc catttcggtc ctgcggtgaa
     1261 caggaatccg aacatgaaga acggcaggaa cgcgttgccc atgagccagc cgtgcatcca
     1321 cgccggcggc agctcgagcg cgagcgggtg gccagccgtg cgcgcggcga tctgcaccag
     1381 ccaccacagc gacgcggtgc cgaggccgag catgccgaag aagaagaaga gtcggtgcgg
     1441 cgcggcgagc agccagcgca acgacgttcc ggcggcgggc gagaagcggt cttcggtcag
     1501 cggcagcgtc ttctgcgtca tcgcgcgcgc ggttgtcgca ggaatgccgg caccagaggc
     1561 ccgatcgcgt cgagggtgtt cttgaccagc aggccgaact ccgtgtcgcc ttccatcacc
     1621 agcgcacgct cgaagaacaa ggtgtcggcg tcctcctctc cgctggccat gcgccagaac
     1681 gcggatgcgg tcgcgctgac gtgcagcgcg acttcggcgc cgcgcggcgc gagcgcgaag
     1741 ccacgcgcgc cgagcgcaag gcgcatcctc acgccgaaat cgctcacgtg cacctcgaca
     1801 caacgatccg agagcgctgc gcgcgcatcg gcgtcgagcc gtggcagcag cacccgattg
     1861 agcaccttgg ccagcacgaa cgacggcggc tcgatcggca accgttcgat caggcggcgc
     1921 acggcgtcgg gcggggcggg cagcggcggc agcgtcgggt tcacgcgtgg ctccactcga
     1981 aacccggatg acggtgtgcg tagccgtcgg caggcgcacc gggaagcccg agttcgatca
     2041 gttcggcgag cgccgcgtcg gcgtcggcgc cgcgattgaa cacgcggtcg aacgcatcga
     2101 tcacctgctc gaagttgtgc gcgatcggcg acagccgcag gcgccgtaca cctgcctcga
     2161 ccacggcact gcgccaagcg atcaggcact gcacgcgggc ggattgcgtc tgcaggccat
     2221 tcaaggtcag gaacgggtcg ccttcctgcg ttttcagcac gagtccgtcc ggatgatcga
     2281 ggcagcggaa ctcacactcg tcgcggttgc gaccgtagtg gcgtgcggtg aaacagcgcg
     2341 ccgagaacgc gagcggcatg cggccgaacg cgaacagctc ggtctcgacg ggcggcgtgc
     2401 ggtcttccgg gttcacggcg gcgatcgcgt cgagcgatag ttcgagcggc ggcacccagc
     2461 gcactgcacc catctgcgca aactcgtcga gggccggtgc gctgtacacg ttcacgtgag
     2521 gcccgatcac gaacggacgc ccgttcatgc gcagcgcggc cgcgctgtcg ttggcctcga
     2581 ccaggaactc gccgttgtcg gtgaagcggt gcagcgtgcg cagttcggcc tcggattcga
     2641 tcagcgcctg gccgctcagc acgacttcct tgccggccgc ggcgagatcg cgcgccagcg
     2701 cgagccagtc gtcgaccttc atctcgtgcc ggcgcgagca gacgatctcg cccagataca
     2761 ccgtgtgcgc cgcggactcg gccacgcgcg catagaactc ggtcagcgtg cggcgcggcc
     2821 agtaggtcag caccggaccg acgctgagct cgaactgcgg caccgtcact tccatggccg
     2881 gctgtaggcg ccgagcgtgt gctgctggcc ttcggcgagc cgcccgagcg ccgcgatcca
     2941 cgcgggctgc ggcgagaaac gcgcgggctg cgcgtgacac gcgtcgatcg cggcgcgcca
     3001 cacgcgcgtg acctgctcga cgtaggccgg gctgcgctgc cggccctcga tcttgatcgc
     3061 ggcgacaccc gcggccatca ggtcgggcag gatcggcagc gtgttcagac tcgtcggctc
     3121 ctcgatcgcg tggccgacct cgccgtcgcc gtcatttccc acggcatagc ggcccttgca
     3181 gaccgtcggg tacgcgcgcg gttcgctggt gccgaaatga ttgatctgga ttccgttcag
     3241 gcgcgcgtcg acgctgtggt cgggatgctc gatccagcgc acgtgcaacg ccggcgagca
     3301 ggcgccgaag ttgttcggcg actgtcccgt cgcgtacgac ga
//
LOCUS       DBNHBY010000431         5535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_635296, whole genome shotgun sequence.
ACCESSION   DBNHBY010000431 DBNHBY010000000
VERSION     DBNHBY010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_635296"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="AC16HB_10710"
     CDS             complement(<1..269)
                     /locus_tag="AC16HB_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607304.1"
                     /translation="MIAGAIERYRATVMPPLRPWRAILLGLAATALFGCPSAPPTTRP
                     AAAFALMGDMPYSAREADAVDMLLAAINADAGVEFVLHVGDIKGSG"
     gene            326..1336
                     /locus_tag="AC16HB_10715"
     CDS             326..1336
                     /locus_tag="AC16HB_10715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR5607305.1"
                     /translation="MTINHQFRLAARPVGLPKRSDWEFTEEPVREPGAGEFLVKVHYI
                     SLDPAMRGWMNDGRSYIPPVALGDVMRAGAAGQVIASNHPQFAVGDYVTGGFGVQEYA
                     MTDGKGVVKVDPKVAPLPVFLGTLGMPGMTGYFGLLEIGRPREGQTVVVSGAAGAVGT
                     VVGQIAKIKGCRAVGIAGGADKCRWIVEELGFDAAIDYKQEKVGAALKQHCPNGIDVY
                     FDNVGGDILDAALARLARGARVIICGAISQYNTTAAVKGPSNYLSLLVNRASMTGMVV
                     FDYADRYALAAREMAGWMMQGKLKARTDVAEGLQNFPDALLKLFRGENTGKLVLKIAA
                     DG"
     gene            1503..2102
                     /locus_tag="AC16HB_10720"
     CDS             1503..2102
                     /locus_tag="AC16HB_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5607306.1"
                     /translation="MDNDRVRQLLVRIGKQDQAAFEELYKAFSRRVYAYALNMLKDHV
                     RAEEVLVDTMHQIWRNPAQFRGDSQFSTWLIGIARNKALMVFRGQRPDEDHADLEDIA
                     ETEAADDAEEGFAQLAQRQRAEGVRHCMDKLSDEHRECMHLVFFEGYSLAEVAAVQRC
                     PENTVKTRLFHARQRIKNCLRLLLEREGADLRLAPRGEG"
     gene            2102..2743
                     /locus_tag="AC16HB_10725"
     CDS             2102..2743
                     /locus_tag="AC16HB_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5607307.1"
                     /translation="MDKRFNELLPWYVNGTLNAEDRAWVDHYLADHPAARAEVEWHQS
                     LKAQIHESAPKVPATIGLAKTMLLIRGDRPTLAERIMGFIGGFGTGPVAAFSMRPALA
                     LGMLAVIVAQGGVIFNMMQHADEDAMQIRALRAVRVDEGPLLKVNFAPESKEADIRLL
                     LVSVEGTLAGGPGQLGDYYVRVPAGKEQQFADRLKSSAIVQSVALAPGLPPRD"
     gene            2753..3619
                     /locus_tag="AC16HB_10730"
     CDS             2753..3619
                     /locus_tag="AC16HB_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR5607308.1"
                     /translation="MDPRARSTGFNGRRRAALRLIATACAATLLPRAPAFAQGGLGAP
                     GERLALVIGNGAYKIGALKNPVADATAVAAALKQLGFTVIQRENTTLRELIEALRDFS
                     QRAANAAVRVVFYAGHGVQAKGRNYLLPVDVDIQSEDEIATRSADVNELVDRLGAIRQ
                     GINIVILDACRVNPFAGGVIVGPDGRRLKFRGATPSGLAKLDAPLGTMVAFSTAPGGV
                     ALDGPSGTHSVYTKYLLGYLATPGMPIEQLFKKVRIAVAEETQRIQVPWESSSLTVDF
                     CFRSDTRGSCGI"
     gene            3709..4203
                     /locus_tag="AC16HB_10735"
     CDS             3709..4203
                     /locus_tag="AC16HB_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607309.1"
                     /translation="MLHLVRALMGLALAATAVAAQARNEHAAAESLDLSTPRFFISIV
                     PHRADVDAVPGFSESAPGMPAFRLTAPRRSRLEFAVQQRDVFAAGDQLQLRLASDAHV
                     FAQLLSGGRISGADEEGMLAMLGVASRVRLSYNNGPWSLSLGATRKLGAGEVTARIAF
                     SARF"
     gene            complement(4204..5160)
                     /locus_tag="AC16HB_10740"
     CDS             complement(4204..5160)
                     /locus_tag="AC16HB_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5607310.1"
                     /translation="MSTEPSERTARPVRLTGLAHLVDALNNFGLIRIGLADRERLGTF
                     TADDGQSIPLQVSGRGHPVVLVHGLGCSHRHWSRVARRLARNHCVFAADARGHGRCVH
                     VESHRITLARLARDLHNLIDTFHLERVVLVGHSMGALTVMQYLRDFGTERVVAATVVD
                     QSPRVVTDDDWRLGLFGGCSAQMLRALIEGARANLAETVLREIEAAAGWAGRWLAPEA
                     LLGRWLRSWLGRVDVAPLLDLAETLAAADFRALMRGLEVPLLVVLGGRSPHYGGLPLD
                     EYYRAAVPRVQVEVYPRSGHSPHYAEPARFARDLERFIATAL"
     gene            5317..5487
                     /locus_tag="AC16HB_10745"
     CDS             5317..5487
                     /locus_tag="AC16HB_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607311.1"
                     /translation="MESTRIELAVNESDWLAGGDGDDYSCDDVRTAWLCAELHRGPTG
                     VKGSFEAVWLDR"
BASE COUNT          929 a         1864 c         1911 g          831 t
ORIGIN      
        1 ccgctcccct tgatgtcgcc cacatgcagc acgaattcga cgccggcatc ggcgttgatg
       61 gcggcgagca gcatgtcgac ggcgtccgct tcgcgtgcgc tgtagggcat gtcgcccatc
      121 agggcgaacg cggctgcggg gcgcgtcgtc ggtggcgcgc tggggcagcc gaagagcgcg
      181 gtcgccgcga gcccaaggag gatcgcgcgc catgggcgga gcggcggcat cacggtggca
      241 cggtagcgtt cgatggcgcc ggcgatcata gcgatgcgac cgctgccgta gactgcctcg
      301 gctttcatcg cctgaggctg ccgtcatgac gatcaaccat cagttccgcc ttgctgcacg
      361 cccggtcggc ctgccgaagc gcagcgactg ggaattcacc gaggagccgg tacgcgaacc
      421 cggcgccggc gagttcctgg tcaaggtgca ctacatctcg ctcgaccccg cgatgcgcgg
      481 ctggatgaac gacggccgct cttacatccc gccggtcgcg ctcggggacg tgatgcgcgc
      541 cggcgcggcg ggtcaggtca tcgcctcgaa tcatccgcag ttcgcggtgg gcgactatgt
      601 gaccggcggc ttcggcgtgc aggagtacgc gatgaccgac ggcaagggcg tggtgaaggt
      661 cgatccgaag gttgcaccgc tgccggtgtt tctcggcacg ctcggcatgc ccgggatgac
      721 tggatatttc gggctgctgg agatcggccg gccgagggaa ggccagaccg tggtcgtgtc
      781 gggcgccgcg ggcgcggtcg gaaccgtggt cgggcagatc gcgaagatca aggggtgccg
      841 cgcggtcggc atcgccggcg gtgctgacaa atgccgctgg atcgtcgaag aactcggctt
      901 cgatgcggcg atcgactaca agcaggagaa agtcggtgcg gcgctcaagc aacactgccc
      961 gaacggcatc gacgtgtact tcgacaacgt cggcggcgac atcctcgacg cggcactggc
     1021 gcggctggcc cgcggcgcgc gcgtgatcat ctgcggcgcc atctcgcagt acaacaccac
     1081 ggcggcggtc aagggcccga gcaattacct gtcgctgctg gtcaatcgcg cgtcgatgac
     1141 cggcatggtc gtgttcgact acgcggaccg ctacgctctc gcggcgcgcg agatggcggg
     1201 ctggatgatg caggggaagc tgaaggcccg aaccgacgta gcggaggggc tgcagaactt
     1261 ccccgacgcg ctgctcaagc tcttccgcgg cgagaacacc ggcaagctgg tgctgaagat
     1321 cgccgccgac ggctgagcac cctgaaaatc ccttgaacct ttcgacgccg gcctgaaaca
     1381 cagggtcgag tgacggcgcg aggtcccgta cccgcagcgc cggtccggtc gcgccccttg
     1441 gcgctccgcc cgcagaggcg cacgctgtaa gcttttcgcg tgccagcggc ggacggagaa
     1501 caatggacaa cgaccgggta aggcagctgc tcgtgcggat cggcaagcag gatcaggccg
     1561 cattcgaaga gctttacaag gcgttcagcc gcagggtcta tgcctatgcg ctgaacatgc
     1621 tgaaggacca cgttagggca gaggaagtcc tggtcgacac gatgcaccag atctggcgca
     1681 acccggcgca gttccgcggc gattcgcagt tcagcacctg gctgatcggg atcgcacgca
     1741 acaaggcact gatggtcttc cgcgggcagc gcccggacga ggaccacgcc gacctcgagg
     1801 acatcgccga gaccgaggcg gccgacgacg ccgaagaggg cttcgcgcag cttgcgcagc
     1861 ggcagcgcgc cgagggcgtg cgccactgca tggacaagct gtccgacgaa catcgtgaat
     1921 gcatgcacct cgtgtttttc gagggttact cgctggccga ggtggccgca gtgcagcgct
     1981 gccctgagaa cacggtcaag acgcgcctgt ttcacgcccg ccagaggatc aagaactgcc
     2041 tgcgcctgct gctcgaacgc gagggtgcgg atctgcggct tgcgccgcgc ggagaaggat
     2101 gatggacaag cgattcaacg agcttctgcc ctggtacgtc aacggcacgc tgaacgccga
     2161 ggaccgcgcc tgggtcgacc actatctcgc cgatcatccg gcggcccgcg ccgaggtgga
     2221 atggcaccag tcgctcaagg cgcagatcca tgaaagcgcg cccaaggtgc cggcgacgat
     2281 cgggctggca aagaccatgc ttttgatccg cggcgaccgg ccgacgctcg ccgagcggat
     2341 catgggattc atcggcggtt tcggaacggg gccggtggcc gccttcagca tgcgcccggc
     2401 gctcgcactc gggatgctcg cggtcatcgt ggcgcagggc ggcgtgatct tcaacatgat
     2461 gcaacacgcg gacgaggacg cgatgcagat ccgtgcgctg cgggcagtcc gcgtcgacga
     2521 ggggccgctg ctgaaggtga atttcgcgcc cgagtccaag gaagccgata tccgactgct
     2581 gctggtgtca gtcgagggca cgctcgcggg cgggccgggg cagctcggcg actactacgt
     2641 acgcgttccg gccggcaagg aacagcagtt cgccgaccgg ctgaaatcca gcgcgatcgt
     2701 gcagtcggtc gcacttgcgc caggcctgcc gccgcgcgat tgaggcgttg ccatggatcc
     2761 gcgtgctcga tcgactggat tcaacgggcg ccgccgcgcc gcgctgcgcc tgatagcgac
     2821 cgcttgcgcc gcgacgctgc tgccgcgcgc accggcgttt gcgcagggcg gcctcggcgc
     2881 gccaggcgaa agactcgcat tggtgatcgg caacggcgcc tacaagatcg gcgccctgaa
     2941 gaatccggtc gctgatgcca ccgccgtcgc cgccgccctc aagcagcttg gcttcaccgt
     3001 catacagcgc gagaacacga cgctgcgcga actgatcgaa gcgctgcgcg acttctcgca
     3061 gcgcgccgcg aacgccgcgg tgcgggtcgt gttctatgcc gggcacggcg tgcaggcgaa
     3121 gggacgcaac tacctgctgc ccgtcgacgt cgatatccag agcgaggacg agatcgcgac
     3181 ccgcagcgcc gacgtcaacg agctggtcga tcgcctcggt gcgatccggc agggcatcaa
     3241 catcgtgatc ctcgacgcct gccgggtgaa cccgttcgcg ggcggcgtga tcgtcggccc
     3301 ggacggccgg cggttgaagt tccgtggcgc cacaccgtcg ggtctggcca agctcgatgc
     3361 gccgctcggc acgatggtgg ccttttccac tgcacccggt ggcgtggcgc tcgacggccc
     3421 gagcggcacg cacagcgtct acaccaagta cctgctcggc tacctggcga cgcccggcat
     3481 gccgatcgag cagctgttca aaaaggtccg catcgcggtc gcggaggaaa cgcagcgcat
     3541 ccaggttccg tgggagtcct cgagcctgac cgtggacttc tgcttccgca gcgacacgcg
     3601 gggcagctgc ggcatctgag ccgccgaatt tcacgatcac acgccgcccg gcgcccgctt
     3661 tgaaccaatg cggcgccggc agaaacgcag gaccaggagg gcacgcatat gctgcacctg
     3721 gtccgggcac tgatgggtct tgcgcttgct gcgacagccg tggcagcgca ggcgcgcaac
     3781 gagcatgccg ctgccgagtc gctcgatctg agcacgccgc gcttcttcat cagtatcgtt
     3841 ccgcaccggg ccgacgtcga cgccgtcccc ggcttcagcg aaagcgcgcc tggcatgccg
     3901 gccttcagac tcaccgcgcc gcgccgtagc cggctcgaat tcgccgtcca gcagcgtgat
     3961 gtgttcgccg ccggcgacca gctgcaactg cgactggctt cggacgcaca cgtcttcgcg
     4021 caactgctct cgggcggacg aatctccgga gccgacgagg aaggcatgct ggcgatgctc
     4081 ggcgtcgctt cgcgggtgcg gctctcctac aacaacgggc cgtggtccct gtcgctgggc
     4141 gccacgcgca agctcggcgc cggcgaagtc accgcgcgca tcgccttctc ggctcgattc
     4201 tgatcaaagc gccgtcgcga tgaagcgctc cagatcgcgc gcgaaccgcg ccggctcggc
     4261 gtagtgcggc gaatggcctg agcgcggata cacctcgacc tgcacgcggg gcaccgcggc
     4321 ccggtagtac tcgtcgagcg gcaggccacc atagtgcggg ctgcgcccgc cgagcacgac
     4381 cagaagcggc acctcgagcc cgcgcatcag cgcgcgaaag tccgcggcgg cgagcgtctc
     4441 ggccagatcg agcagcggcg cgacgtcgac ccgcccgagc caggaccgca gccagcgacc
     4501 gagcagggct tcgggcgcga gccagcgccc ggcccagccc gccgccgcct cgatctcgcg
     4561 cagcacggtc tcggccagat tggcgcgtgc gccctcgatc agcgctctga gcatctgcgc
     4621 gctgcagccg ccgaagagtc ccagccgcca gtcgtcatcg gtgacgacgc gcggcgactg
     4681 atcgaccacg gtcgctgcca cgacgcgttc ggtgccgaaa tcgcgcaggt actgcatcac
     4741 ggtcagcgcg cccatcgagt ggccgacgag caccacccgc tcgaggtgaa aggtgtcgat
     4801 caggttgtgc aggtcgcgcg ccagccgcgc cagcgtgatg cgatgcgatt cgacgtggac
     4861 acagcgcccg tggccgcgcg cgtcggccgc gaatacgcag tggttgcgcg cgagccttcg
     4921 cgcgacgcgc gaccagtgcc ggtgcgagca gccaagtccg tgcaccagca cgaccggatg
     4981 cccgcggccg ctgacctgca gcggtatcga ctgcccgtcg tccgcggtga acgtgcccag
     5041 ccgttcgcgg tccgccagcc cgatgcggat caggccgaaa ttgttcagcg cgtcgaccag
     5101 gtgcgcgagc ccggtcaggc ggaccgggcg cgccgtgcgt tcggaaggct cggtgctcat
     5161 cgtcgcgtgg ccaggggcgc gtgaccggcc tgcgccgcgc ccggatcctg ctcctgcaat
     5221 tcgccgagtc gacttcgatt gaaccgttgg ccgccgcagc gtaactcagt gacaggttcg
     5281 gcggcatacg ccgacaaact gcaaacggag gcaacgatgg aatcgacgcg tatcgaactg
     5341 gccgtcaacg aatcggattg gcttgcgggc ggcgacggtg acgactattc gtgcgacgac
     5401 gtgcgcaccg catggctgtg cgccgagctc caccgcggac cgaccggggt caagggcagc
     5461 ttcgaagcgg tctggctcga tcgctgagcg acccgccggg cgccgcaccc ccgcaacacc
     5521 ccactccttc ttggc
//
LOCUS       DBNHBY010000432        27130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1718567, whole genome shotgun sequence.
ACCESSION   DBNHBY010000432 DBNHBY010000000
VERSION     DBNHBY010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 27130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27130
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1718567"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16HB_10750"
     CDS             complement(<1..226)
                     /locus_tag="AC16HB_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607312.1"
                     /translation="MKFSRVCLAALATSCLAAAPFASNAALTLRLSDGVTTVQVVDSD
                     GDGFVNYTGAVGSNWFLNVSTGLGSGILDAG"
     gene            complement(556..1656)
                     /locus_tag="AC16HB_10755"
     CDS             complement(556..1656)
                     /locus_tag="AC16HB_10755"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HIR5607313.1"
                     /translation="MIRGNDPILLTPGPLTTSLATKQAMLRDWGSWDAAFNQITLSLC
                     RDLVDIVGGGTDYVCVPLQGSGTFSVEAAIGNVVPRGGKVLVPNNGAYCARIVKICSY
                     LNRAVVDLPVPEDRPATAAIVDAALRADPSITHIAQVHCETGAGMLNDLNGIAQVCKK
                     YGKGLIVDAMSSFAALPINVADAPFDALVAASGKCLEGVPGMGFVIMKKSVVEGCAGN
                     SHSLAMDLADQYAYMQKTTQWRYTPPTHVVAALRAAVDQFNAEGGQRARLARYTENCR
                     TLVAGMRRLGLRTFLPDELQAPIIVTFHAPTDPAYDFKRFYAAVRDRGYILYPGKLTK
                     VETFRVGCIGAVDSNEMRNVVSAIELALGPHR"
     gene            complement(1802..3520)
                     /locus_tag="AC16HB_10760"
     CDS             complement(1802..3520)
                     /locus_tag="AC16HB_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HIR5607314.1"
                     /translation="MSFALERTFEPRRLDLWREALVARGGLVLLVVALFVFLTVPLAT
                     LLVRSLEDRAGTFVGLTNFVTYFASPAFARSATNTLVFAGLTTLATVPLAFGFAYAIQ
                     RSCIPAKNLWRNIAMIPILAPSLLAALSFIYLFGNQGWLKGVLGWFGLTTVYGMPGMV
                     LAMTFASFPHAVMILLAALALSDARLYEVADSLATSEWRKFVTITLPGARYGLISAAM
                     VVFTMAVSEFGVPKVIGGNTSVLAIDIYKQVIGQQNFSMGAVVGLILLAPAVAAFFVD
                     HVVRTRQKALLTARSVPYRARPSFARDSLLLLYVVTIAALLLLVLGMAVFGSFVKIWP
                     YNLSFTFNHYTYGFEEAGVDHAWRSSVVMAAWCAAFGAAITFAGAYWVEKTRGVDWLR
                     PLIQLQAMMPMAVPGMVLGIGYILFFNHPANPLNFLYGTMTILVASTIVHFYSSSHLT
                     AVTALKQLDNEFESVSASLKVPFYKTFWRVTVPVCLPAVVDISRYYFVNAMTTISCVV
                     FLYAPKTMPASISILHLDEAGAVGSAAAMATLIVATSAVVTTLFFVVEGWLIRRTQAW
                     RDTTRS"
     gene            complement(3594..4670)
                     /locus_tag="AC16HB_10765"
     CDS             complement(3594..4670)
                     /locus_tag="AC16HB_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5607315.1"
                     /translation="MEALRLEKVHKSFGTFVALKSIDLAITSGELVCFLGPSGCGKTT
                     LLRIIAGLEPQTRGRVIQNGRDVSNAPPAARDYGIVFQSYALFPNLTIRANVAYGLVN
                     RRKARVEIEARVKELLTLVGLPDAGPKYPAQMSGGQQQRVALARALATSPSLLLLDEP
                     LSALDAKERERLRGEIRALQQRLKVTTIMVTHDQEEALSMADRVVVMNHGVIEQVGTP
                     MEAYEQPATPFVADFLGKINVLRAVALGGGRFRVGQVELQTQANGVATGANVKLYLRP
                     EDRILPDTGSVEAMPNSLRATVRSLDFLGTFCLANVDVEGFDGQRLMLYFSLNQALEM
                     GVRAGAQLPFALRPERFRVFDQSA"
     gene            complement(4785..5813)
                     /locus_tag="AC16HB_10770"
     CDS             complement(4785..5813)
                     /locus_tag="AC16HB_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5607316.1"
                     /translation="MALRRVAVGLAAGLIGSLFTFGALAQKTQLTVYTALETDQLKAY
                     QAGFEKANPNIEIVWVRDSTGVITAKLLAEKANPKADLVMGVAATSMAIFDTEGMLVP
                     YAPAGLNRIAPQYRDSKNPPTWVGMDVWGATICFNTAEAAKRNIPKPETWRDLLKPVY
                     KGQIVMPHPASSGTGFFDVSAWLQLWGEAEGWKYMDGLHENIAQYMHSGSRPCAAAAN
                     GEYVVGISFEYRANREKAQGKPIELVFPKEGLGWDLEASGIHKGTKNLAAAQKLMDWL
                     ATDDAMALFAANFAIVAVPSLSKPLPNVPADYGSRLVKNDFLWAAKNRDRILAEWSKR
                     YESKAAPK"
     gene            6043..6795
                     /locus_tag="AC16HB_10775"
     CDS             6043..6795
                     /locus_tag="AC16HB_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate utilization associated
                     transcriptional regulator"
                     /protein_id="HIR5607317.1"
                     /translation="MPSRSPAAPPSGGNVVAHPTIALLRTRSLASAVQQEIERMILAG
                     ELAPGTKLTEAWLSEKLGVSRGPLREAFRMLEEAGLVRQEKNRGAFVRDIPVEEAVEI
                     FDLRAVMDELVGRTVATTVTPEQGRQLKTLVEQMEQAARASDADAYHLLNLQFHDQLV
                     EYAGNRKMAAIYRKLVKELALFRRRNLAQQQALPQSVAEHRQILKAIQSGDAEAAGRA
                     MFAHVIESKQRTLRAESERRPPARVRAVSRRG"
     gene            6802..8013
                     /gene="phnA"
                     /locus_tag="AC16HB_10780"
     CDS             6802..8013
                     /gene="phnA"
                     /locus_tag="AC16HB_10780"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HIR5607318.1"
                     /translation="MLEVNGRRYAAPRQPIVVICVDGCEPDYIAQSVAAGAMPTMKRW
                     LAEGTALIADSVVPSFTNPNNLSIVTGAPPSVHGICGNFLYDPEANAEVMMNDPKWLR
                     APTLLACLADAGHSVAVVTAKDKLRQLLGHRLRGICFSAEKADQASVAVNGIDAVPSL
                     VGMPVPGVYSAELSEFVFAAGVRLLETRRPDVMYLSTTDYIQHKHAPGDAVANAFYAM
                     MDRYLARLDALGAVVVLTADHGMNAKTWLDASPNVIYLQDAIDGWIGSQRARVILPIT
                     DPYVVHHGALGSFATVYLPADALSPVADRVSATPGIEMVLTRTQAADRFELPADRIGD
                     LVVVSERSVVLGTSKSRHDLSQLEMPLRSHGGISEQRVPLIVNRRIDGLDGRRRWRNF
                     DAFDLALNRAQ"
     gene            8010..9446
                     /gene="phnY"
                     /locus_tag="AC16HB_10785"
     CDS             8010..9446
                     /gene="phnY"
                     /locus_tag="AC16HB_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HIR5607319.1"
                     /translation="MNAPLPIKHESLRIAGRKFEGHGHFEVRYPYTNEVIATVAKASV
                     EDVRRALSIARGFRSKLSRHDRYRILMRTGEIIAARKDEIARGITLESGLCLKDSLYE
                     VGRASDVLLFAANQALVDDGQVFSCDLTHHGKSRKVYTLREPLLGAIAAITPFNHPLN
                     QVIHKVAPAVATNNRVVLKPSEKTPLAAFILADALYEAGLPPEMLSVVTGDPREIADE
                     LLTSPDVDLVTFTGGVPIGKYIAAKAVYKRQVLELGGNDPLIVMEDADIEEAATLAAS
                     GSYKNSGQRCTAVKRMLVHEKVADRFVELLVAKTKAVRFGDPLDMATDMGTVIDEAAA
                     QDFEARVNDAVARGARVLVGHERRGALYPPTVIDQVTPDMNVVKTETFGPVSPVIRIR
                     DIDEAIRVANSTAYGLSSAVCTNRLDYITRFIAELNVGTVNVREVPGYRLELTPFGGI
                     KDSGLGYKEGVLEAMKSFTNVKTYSLPW"
     gene            9462..11141
                     /locus_tag="AC16HB_10790"
     CDS             9462..11141
                     /locus_tag="AC16HB_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR5607320.1"
                     /translation="MQALLNLLAAVALLVWGTHIVRTGVLRLLGANLRQVLRGSSDRR
                     YMAFLAGIGVTGLLQSSTATALLVSSFVSQGVIATSAALAVMLGADVGTALMVQVFSL
                     NLSWLAPLAIIAGVIVYLSKKNEHAGQIGKVVLGLGVMILALQLIVQATAPLAQAAGV
                     KVLFATLTGDVLLDVLVGALFAIAFYSSLAVVLLTATLTASHVLSLPVALAIVLGANL
                     GGGLIALLTTLRAGPIARRVPLGNLLFKLAGCAIAIPLLSFGLEWLAKLDGDPKAIVV
                     NAHLAFNLMLALLFIQFVGRSAQAVERLLPAPHAADAPGTPKHLDPVALSTPPLAIGN
                     AAREAIRLADVVQDMLDGLLRVLKSNDFQLAERLCARDDEVDRLYTAIKLYLTQISRE
                     ALEAAESKRWADIIQFAINMEHAGDIIERVLVDIAEKKIAHGLSFSDAGMREIEDLHA
                     RLLGNLKLAVAVFLHGDLKSAQQLIAEKVSFRELEMRYADNHLQRLSGNSPQSVETSS
                     LHLDLLSDFKRINSLFCAMAYPILEQAGVLSRTRLVGEQPALFAADEHRHA"
     gene            11138..11968
                     /locus_tag="AC16HB_10795"
     CDS             11138..11968
                     /locus_tag="AC16HB_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5607321.1"
                     /translation="MKDWHRYSGTISEAERREFVRFMHELIGAAFAVIRPFFLSGTAV
                     MTKGDASPVTLADRGAEEAMRTLIEVRYPEHGILGEEFGAKMGRGYRWVLDPVDGTRA
                     FITNCFLFGTLIALERDNGAGFKPILGALAHPAAGVALIGHSGQTSLYASDGSSRAVH
                     VRSCRQLENATVLATTHWSTGEQRAGAAVENVIRRAKLYRTWGDCFGYFALCTAGADV
                     MLDPTLAYWDVAAIVPVVEGAGGRVTSWRGGDPLDDPSLLATAGGLQDEILQIIQAAA
                     "
     gene            complement(12068..12514)
                     /locus_tag="AC16HB_10800"
     CDS             complement(12068..12514)
                     /locus_tag="AC16HB_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5607322.1"
                     /translation="MASVNKVILIGNLGRDPETRYSADGAAIANVSVATTRRYKDSSG
                     QQQEETEWHRVVFFGRLAEIAGEYLRKGRSVYVEGRLRTRKWTDKEGAEKQTTEIVAE
                     NMQMLGSREGMGGGDIEESAPPARPAAKPAAKPAQNIAEMDDDIPF"
     gene            12636..15530
                     /gene="uvrA"
                     /locus_tag="AC16HB_10805"
     CDS             12636..15530
                     /gene="uvrA"
                     /locus_tag="AC16HB_10805"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5607323.1"
                     /translation="MDAIKVRGARTHNLKNISLDLPRKRLIVLTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLQLMEKPDVDSIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EVHDYLRLLYARIGTPYCPDHPEHPLAAQSVAQMVDSALALPADTRLVVLAPIVRERK
                     GEFEDLFDDLQTQGFVRFRVDGAIVEPNDLPHLAKNEKHSIDVVVDRLKVRPEAKQRL
                     AESFETALRIAAGRALLLDTDNGTETVFSNRFACPICGYALAELEPRLFSFNNPVGAC
                     PSCDGLGTVEYFDEQRVVVHPELTIASGAIKGWDARNQYYFQMLAALAKHAQVDIDLP
                     WGALPESFRTQVLLGSGTEVLPFAYTNDKGRRIVRHHAFEGVLPNMERRWRETDSAAV
                     REELAKCRAVKPCPACDGTRLKREARFVFVGNGPQKRAIFEIERAPLAQTLAYFDALE
                     LEGAKREIGARIVREIGARLRFLNDVGLTYLTLDRSADTLSGGESQRIRLASQIGSGL
                     TGVMYVLDEPSIGLHQRDNERLIATLKHLRDLGNTVLVVEHDEDAIRAADHVVDMGPG
                     AGEHGGEVVAQGTPSDIENSPASLTGAYLARRRAIAVPAARVAPRLHWLRLAGAHGNN
                     LKHVDLELPMGLLVCVTGVSGSGKSTLINDTLAAIVARHLYGSAVLAAPYESVEGLEH
                     FDKIVMVDQSPIGRTPRSNPATYTGLFTPIRDLFAGTPTARERGYEAGRFSFNVKGGR
                     CETCQGDGLIKVEMHFLPDVYVPCETCHGQRYNRETLEVLYKGRNIAEVLDLTVEQAA
                     QFFSAVPVLAKKLQTLADVGLGYIRLGQSATTLSGGEAQRVKLSLELSKRDTGSTLFI
                     LDEPTTGLHFHDIELLLHVLHQLRDAGNTVVVIEHNLDVIKTADWVIDLGPEGGEGGG
                     RVVAAGTPEAIAAHSGSHTGRFLARVLTRAEERETRRAPARRHSGGLAVS"
     gene            complement(15709..17691)
                     /locus_tag="AC16HB_10810"
     CDS             complement(15709..17691)
                     /locus_tag="AC16HB_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR5607324.1"
                     /translation="MPTALELTLFLLAASVAGVVAFRLLDLPPTLAYLVVGILIGPHA
                     LGVVPDSADARYLAEFGVVFLMFSIGLEFSLPKLKSMRRIVFGLGLAQVVATTVLVTA
                     AGWMMGSHLGLTRAASFVLGATLAMSSTAIIVKLLVDRLELETEHGKRIVGVLLFQDR
                     AVVPLLVVIPALATEPDKLGAALMWAAAKAAILLGLLLVGGQWLIRHWLHLVARRKSH
                     ELFTLNLLLIVLGLAWLTEHAGLSLALGAFVAGMLISETEYRHQVAEDIKPFRDVLLG
                     LFFVTIGMLLNVRIVIENIGWVVLALLIPSAFKCGLIVALARMFRVGPGTAIRTGLAL
                     ATAGEFGFVLLSESGALLAPDVKQIVLAAMLLSMLAAPFLVHYSDRIAMRFASAEWML
                     RALQLTQVATRSMATERHVIICGYGRSGQHLAKMLEQENLGYVALDLDPDRVREAAAA
                     GDSVVFGDAGRRETLIAAGLMRAAALVVSYNNSASALKVIHFAHEARPALPIIVRTQD
                     DADLDRLLRAGATEVVPEIFEGSLMLGSHALVLLGVPLQRVVRRVREARDSRYRLLRG
                     YFHGADDPAEFEDESHVRLHSLPIEAGAAAVGAMLGSLGLRSIGVEVTAVRRRGIRGA
                     DPSDEMVLQVGDVVVLRGAPEALELAEERLLQIKTA"
     gene            17824..18810
                     /locus_tag="AC16HB_10815"
     CDS             17824..18810
                     /locus_tag="AC16HB_10815"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR5607325.1"
                     /translation="MEDARTRPRAPALELAREVLAIESQSVAALADRLDGRFLRAVDL
                     LLACRGRIVVSGVGKSGHIGRKIAATLASTGTPALFVHAAEAAHGDLGMITPADVLIA
                     LSYSGETAELLTIVPLVKLEGTPMIAITGNPESSLARHADVHLDVRVDKEACPLNLAP
                     TSSTTATLALGDALAIACMEARGFGPSDFARSHPGGALGRRLLTRVADIMRTGDAVPS
                     VPIDASVLDAVAEIGRKRLGMTAILARDGSLAGIFTEGDLRRLIERVGDVRALRVAEV
                     MTRKPLTVPSAMLAAEAARILDASLRNQLLVIDDGRLVGALHMHDLTAAKVL"
     gene            18810..19352
                     /locus_tag="AC16HB_10820"
     CDS             18810..19352
                     /locus_tag="AC16HB_10820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR5607326.1"
                     /translation="MPDDAAARAANVRLLILDVDGVLTDGRLYFGPQGEALKIFDVRD
                     GHGIKLLLEAGIEVAILSSRRSEAVEVRARELGIAHVLQGEADKRAGLQRLLAALRLS
                     PEQCAFIGDDWPDLAVLKRVGLAATVADAAPEVRDAAHWIAPAGGGRGAVRQLAELIL
                     RAQGKFDAMLARHSRLGGHA"
     gene            19502..20083
                     /gene="lptC"
                     /locus_tag="AC16HB_10825"
     CDS             19502..20083
                     /gene="lptC"
                     /locus_tag="AC16HB_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR5607327.1"
                     /translation="MRDRITMIIAIALLAIVTATSYWYSRVMRKPESAEPPRPGTPDI
                     VVDRVVLTQFDEQGRARNKLLGERLRHFAENDDIEITAPRLVSLRPDQPRVEARANAA
                     RVENAAERLHLNGDVVITRAAFAGQPEMRMTTEYLLVLPDEDRYRTDRPVKLTRGASM
                     IRANAMDFDNIARIVILTGAVHSVIEPRPAARR"
     gene            20087..20686
                     /gene="lptA"
                     /locus_tag="AC16HB_10830"
     CDS             20087..20686
                     /gene="lptA"
                     /locus_tag="AC16HB_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR5607328.1"
                     /translation="MRASFLRWLLTALALASAPAWADRADRDKPTQIDADAWRGEELK
                     QVVVYSGKVVLTKGTLRITGDRLELREDPEGYQYAVVTARPGELATFRQRRDPTQPGV
                     EEFIEAEAERLEYDGKAETIKLSGRAQVRRIESGEPRDEFRGGVITYNMRESRYSGQG
                     GEAAEGGRTRTIIAPRSAAPAAPPTPLAPARTLEPERRK"
     gene            20719..21477
                     /gene="lptB"
                     /locus_tag="AC16HB_10835"
     CDS             20719..21477
                     /gene="lptB"
                     /locus_tag="AC16HB_10835"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR5607329.1"
                     /translation="MRGSLARGRLVVEHLRKQYGTRVVVKDVSLTVEAGEVVGLLGPN
                     GAGKTTCFYMVVGLVPMDGGTISLDDTSIAHLPIHRRARLGLSYLPQEASVFRRLTVE
                     ENVRAVLELQVDANRKPLAPARVREQLDALLDELQIAHLRTNTAQSLSGGERRRVEIA
                     RALAARPRFILLDEPFAGVDPIAVIEIQRIVRFLKDRGIGVLITDHNVRETLGICDHA
                     YIINEGQVLAAGAPHEIVENDSVRRVYLGEHFRM"
     gene            21488..22960
                     /locus_tag="AC16HB_10840"
     CDS             21488..22960
                     /locus_tag="AC16HB_10840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR5607330.1"
                     /translation="MKPSLQLKLSQHLALTPQLQQSIRLLQLSTLELNQEIEQALTDN
                     PLLERDDDPLATSMRISSDGAIVDKTPPVEAGDAAQATPTTEANGAEHAEGEAALHDW
                     GSATGGPTDDDDSSPLSWAATRTTLAEHLKAQLATTQVSARDRVLVELLIEALDEGGY
                     LTSTLEEVRQMCPPDAGIEFEELTAALKLLQSFDPPGVGARTPSECLLIQLELLSRHA
                     TPPCSAQVLQHAKAIVSEHLPLLANRDYAKLRRLLGASDEDLRTAHQVIRGLNPHPGV
                     GFGAETIDYVVPDIFVRRIKGRWVAQLNPDVMPRLRVNQHYASIVKRDRGKEGQAPWS
                     ARLQEARWLIRNIQQRFDTILRVAQAIVDRQHNFFTHGAIAMRPLVLREIASTVNLHE
                     STVSRVTASKYLSTPFGVFELKYFFGSHVATETGGAASSTAIRALIKQLIGAENPKEP
                     LSDNRIAELLGEQGIVVARRTVAKYREALRIPPVAMRKVL"
     gene            23042..23374
                     /gene="hpf"
                     /locus_tag="AC16HB_10845"
     CDS             23042..23374
                     /gene="hpf"
                     /locus_tag="AC16HB_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR5607331.1"
                     /translation="MNLIIHGHHVEVTPALRGYVEGKMERIRRHFDHLIEADVVLSVE
                     DKLRQRAEITLRVRGNSLHAEATEEDMYAAIDGLMDKLDRQVLRHKDRVRNHVRDPLK
                     HQSAQQQQ"
     gene            23707..24177
                     /gene="ptsN"
                     /locus_tag="AC16HB_10850"
     CDS             23707..24177
                     /gene="ptsN"
                     /locus_tag="AC16HB_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR5607332.1"
                     /translation="MNLISRLMPPSNVLLGLAASSKKRVFEQAGLLFENNQGIGRAAV
                     FDSLFARERLGSTGLGQGVAIPHGRIKGLKDAVAAFIRLEEPVPFDAPDGRPVQLLIF
                     LLVPEQATQQHLDILSELAQMLSDKTFREGLLTATDPMAVHEMLASWEPVRPAA"
     gene            24211..25140
                     /gene="hprK"
                     /locus_tag="AC16HB_10855"
     CDS             24211..25140
                     /gene="hprK"
                     /locus_tag="AC16HB_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR5607333.1"
                     /translation="MPTIQSLFEENADRLRLRWIAGRKGGERKFQGSGALDVPAADLV
                     GHLNLIHPNRLHVLGGPEVRYVARMDAARRAHYADELVTGGALGLIVAEDLSFPPDME
                     SAAETAGLAVFSSPLPAANVIDLLRIYFGKVLAAHCTMHGVFMDVLGMGVLISGESGV
                     GKSELGLELISRGHGLVADDVVDFARVAPDAIEGRCPSLLSNLLEVRGLGLLDIKSIF
                     GETAVRRKMKLRLIVELRRRAAAEMMERLPLAGTTEEVLGLPIHKVVIPVAAGRNLAV
                     LLEAAVRNTILKLRGIDTMADFLERQRQAMDDI"
     gene            25246..25542
                     /locus_tag="AC16HB_10860"
     CDS             25246..25542
                     /locus_tag="AC16HB_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HIR5607334.1"
                     /translation="MLKQVLIAACLVLPVAAMAADEKKMTPQQQKMSMCNKEAGEKKL
                     EGDARKGFMKECLSAKPAAKMTQQDKMKSCNKDAGAKNLKGDERKKFMSECLKG"
     gene            25634..26500
                     /gene="rapZ"
                     /locus_tag="AC16HB_10865"
     CDS             25634..26500
                     /gene="rapZ"
                     /locus_tag="AC16HB_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR5607335.1"
                     /translation="MRVVLVTGMSGSGKSVAIRLLEDLGYYCIDNLPSRFLGQVCAYL
                     SDAGHNEVAVSVDARTEISLSQLPGVIDELRASGNDVRVLFLTASTEALVQRYSESRR
                     RHPLSERLALGTDGLEPTLTESIEAERDLLAPLNSIGHLIDTSALQANTLRTWVRQFA
                     DSPRATLTLTFESFAYKNGIPVDADLVFDVRNLPNPFYDPRLKPLTGLDEPVIAFLAS
                     SKRVDEMIEDIGRFLGRWLPGYVQDSRHYVTVAIGCTGGQHRSVYVVEQLARRFAGQG
                     RVLVRHRGIARH"
     gene            26515..>27130
                     /locus_tag="AC16HB_10870"
     CDS             26515..>27130
                     /locus_tag="AC16HB_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5607336.1"
                     /translation="MRLPLFPLNAVLFPGGSLPLRVFEARYMDMVRDCLRNSSQFGVC
                     LITRGRETGKQAECEPVGCMASIAAWDMQKLGVLNIRTLGTQRFRVRHAAAQPDGLLV
                     GDIEEIAADVDAPLAPEHQPCATLLARIIDDLNAQREAALRDHPDDAGMLAANAIERP
                     HMLHSSVWVGNLLCEVLPVSLRAKQKLMELDDARTRLDIVQRFLR"
BASE COUNT         4777 a         9024 c         8988 g         4341 t
ORIGIN      
        1 tgccggcgtc gaggatcccg ctcccgaggc cggtcgacac gttaaggaac cagttggagc
       61 cgacggcccc ggtgtagttc acgaacccgt cgccgtcgct gtcgacgacc tgaaccgtcg
      121 tcacgccgtc gctgagcctg agcgtcaacg ccgcattcga cgcgaacggc gctgcagcga
      181 gacaggacgt ggctagtgca gcaaggcaga ccctcgaaaa cttcactttg ctctccttcg
      241 gtgttggcac cggttgactt gtgcctgaca ctcagcaaga gcaatgccac ccatgtgaat
      301 caacaacttg gatctgcgct ggaacttgcc gcgtaaagaa ttccgtcata ggaatacggg
      361 atcacgcggt cccgaatccg tcccgcattc aagcggtcga tctcgaccgg caaacggtgc
      421 gatggagccg cttacgccta gaaatcagag gcttgagaag aacggcactg taagaaatac
      481 cgtcacgccg atgcgggagg agcccaaagc ccggccgccc tatcaccgtg cggccggcgg
      541 agaccgatca acggttcagc ggtgcggacc cagagccagt tcgatcgcgg agacgacgtt
      601 tcgcatctcg ttcgaatcga cggcgccaat gcacccgacc cggaacgttt cgaccttcgt
      661 gagctttccc ggatacagga tgtagccgcg gtcccggacg gcggcgtaga agcgtttgaa
      721 gtcataagcc ggatccgtgg gcgcgtggaa cgtgacgatg atcggcgcct gcaattcgtc
      781 cggcaggaac gtccgcaggc ccaggcgccg catgccggcg acgagcgtgc ggcagttctc
      841 cgtgtagcgc gcgagccggg cccgctgacc gccctcggcg ttgaactggt cgaccgcggc
      901 gcgcagcgcc gcgaccacgt gcgtcggcgg cgtgtaacgc cactgcgtgg tcttctgcat
      961 gtacgcgtac tggtcggcga ggtccattgc cagtgaatgc gaattgccgg cgcagccctc
     1021 gaccacgctc ttcttcatga tcacgaaacc catgcccggc acgccttcca gacacttgcc
     1081 gctggccgcg accagcgcgt cgaacggcgc gtccgcgacg ttgatcggca acgccgcaaa
     1141 cgaactcatc gcgtcgacga tcagcccctt gccgtacttc ttgcacacct gagcgatgcc
     1201 gttcagatcg ttcagcatgc cggcgccggt ctcgcagtgc acctgtgcaa tgtgcgtgat
     1261 cgatgggtcg gcgcgcagcg ccgcgtcgac gatcgcggcg gtcgccggcc ggtcctcggg
     1321 caccggcaga tcgacgacgg ctcggttcag gtaactgcag atcttcacga tccgcgcgca
     1381 gtaagcaccg ttgttcggca ccagcacctt gccgccgcgc gggacgacgt tgccaatggc
     1441 ggcctcgacc gagaacgtgc cgctgccctg caagggcacg cacacgtagt cggtgccgcc
     1501 gccgacgatg tcgacgaggt cgcggcacaa cgaaagggtg atctggttga acgccgcgtc
     1561 ccacgagccc cagtcgcgca gcatcgcctg cttggtcgcg agcgacgtcg tcagcgggcc
     1621 gggggtgagc agtatcgggt cgttgccgcg gatcatgaag ggttccttag ggcctattag
     1681 cgcctggctc gcgcatgcgt cgttgcgctg cctcgacagg gagccgccct gccggcggca
     1741 acgcgcctag attgcgggct ttctgggggt cacgcacgtg cgatccaagc gttaacaggc
     1801 cctagctgcg cgtcgtatcg cgccacgcct gtgtgcggcg gatcagccac ccctcgacga
     1861 cgaagaagag cgtcgtcacg acggccgacg tggcgacgat cagcgtcgcc atcgcggccg
     1921 ccgagccgac cgcgcccgct tcgtcgaggt gcaggatcga aatcgacgcc ggcatcgtct
     1981 tcggcgcata caggaatacc acgcacgaga tcgtcgtcat cgcgttcacg aagtagtagc
     2041 ggctgatgtc gacgaccgcg ggcaggcaca ccggcacggt cacgcgccag aaggtcttgt
     2101 agaacggcac cttcagcgac gccgacaccg actcgaactc gttgtcgagc tgcttgagcg
     2161 ccgtgaccgc ggtcagatgg ctcgacgagt agaagtgcac gatcgtcgac gccacgagga
     2221 tcgtcatcgt gccgtacagg aagttcagcg gattggccgg gtgattgaag aacaggatgt
     2281 agccgatgcc gagcaccatg cccggcaccg ccatcggcat catcgcctgc agctggatca
     2341 gcggtcgcaa ccagtcgaca ccgcgcgtct tttcgaccca gtaggcgccg gcgaaggtaa
     2401 tcgccgcacc gaaggcggcg caccacgccg ccatcaccac gctgctgcgc cacgcgtggt
     2461 cgacgccggc ttcctcgaag ccgtatgtgt agtggttgaa cgtgaacgac aggttgtacg
     2521 gccagatctt gacgaacgag ccgaacaccg ccatgccgag caccagcagc agcagcgcgg
     2581 cgatggtcac gacatacagg agcagcagcg aatcgcgcgc gaacgacggc cgcgcgcggt
     2641 acggcacgct gcgcgcggtc agcagcgcct tctggcgtgt gcgtaccacg tggtcgacga
     2701 agaacgccgc cacggcgggc gcgagcagga tcaggccgac cacagcgccc atcgagaagt
     2761 tctgctgccc gatcacctgc ttgtagatgt cgatcgcgag cacgctggta ttgccgccga
     2821 tcaccttggg cacgccgaac tcggacacgg ccatcgtgaa cacgaccatc gccgccgaga
     2881 tcagcccgta gcgtgcgccg ggcagcgtga tcgtgacgaa cttccgccat tcggaggtcg
     2941 ccagcgaatc ggccacctcg tacaggcgcg cgtccgacaa cgcgagtgcc gcgagcagga
     3001 tcatgaccgc atgcgggaag gacgcgaacg tcatcgccag caccatgccc ggcatgccgt
     3061 agaccgtcgt cagcccgaac cagccgagca cgcccttcag ccacccctga ttgccgaaga
     3121 gatagatgaa cgacagcgcg gcgagcagcg acggcgccag gatcgggatc atcgcgatgt
     3181 tgcgccacag gttcttggcc ggaatgcagc tcctctggat cgcgtacgcg aatccgaacg
     3241 cgagcggcac cgtcgcgagc gtggtcagtc ccgcgaagac gagcgtgttg gtagcggagc
     3301 gcgcgaatgc gggcgaggcg aaataggtca cgaaattcgt cagcccgacg aacgtaccgg
     3361 cgcgatcctc gagactcctc accagaagcg tggcgagcgg aaccgtcagg aacacgaaca
     3421 acgcgacgac cagcagcacc agcccgccgc gcgcgaccag cgcctcgcgc cacaggtcca
     3481 agcggcgcgg ctcgaaggtc cgctcgagcg cgaagctcat gtcgtcccct tcgcggtcgc
     3541 ggcgcaaccg ccccgagggg gcgcgaggct tcctgttgcg gcccggcaat gcctcatgcg
     3601 gactggtcaa agacccgaaa gcgctccggc cgcagagcga acggcaattg cgcgcccgcg
     3661 cgcacgccca tttcgagcgc ctgattcagc gagaagtaaa gcatcagccg ctgaccatcg
     3721 aagccctcga catcgacgtt ggcaaggcag aacgtgccga ggaagtcgag gcttcggacc
     3781 gtcgcgcgca gcgaattcgg catcgcctcg accgatccgg tgtcgggcag gatgcggtcc
     3841 tccggccgca ggtacagctt cacgttcgcc ccggtcgcca cgccgttggc ctgcgtctgc
     3901 agttcgacct gcccgacgcg gaagcgcccg ccgccgagcg cgactgcgcg cagcacgttg
     3961 atcttgccga ggaagtcggc cacgaatggc gtcgccggct gctcgtacgc ctccatcggc
     4021 gtgccgacct gctcgatcac gccgtggttc atcaccacca cgcgatcggc catcgacagc
     4081 gcttcctcct ggtcgtgcgt gaccatgatc gtggtgacct tcagccgctg ctgcagcgcg
     4141 cggatctcgc cgcgcaaccg ctcgcgctcc ttggcgtcga gcgccgacag cggctcgtcg
     4201 agcaggagca ggctgggcga ggtcgcgagc gcgcgcgcca acgcgacccg ctgctgctgg
     4261 ccgccggaca tctgcgccgg gtatttcggc ccggcgtccg gcagccccac gagcgtcaac
     4321 agttctttga cgcgcgcctc gatttcgacg cgcgccttcc tgcggttgac caggccgtat
     4381 gcgacgttgg cgcgaatcgt caggttgggg aacagcgcgt acgactggaa cacgatccca
     4441 tagtcgcgcg ccgccggcgg cgcgttggat acgtcgcgcc cgttctggat cacgcgcccg
     4501 cgtgtctgcg gctccagccc ggcgatgatc cgcagcagtg tcgtcttgcc gcagccggac
     4561 ggtccgagaa agcagaccaa ctcaccggag gtgattgcca ggtcgatcga cttcagcgcg
     4621 acgaacgtgc cgaacgactt atgaactttt tcgagccgca gcgcttccat gcgaaaacac
     4681 gacggcggct gcgccgccta tgacgcccgc tcgcacgggc tatgacggat catcataggc
     4741 gcgcagcggc gtcacgacga cggcaggccg catcagccat cagctcactt cggcgcggcc
     4801 ttcgactcgt accgtttgct ccactccgcc aggatgcggt cgcggttctt ggccgcccac
     4861 aagaagtcgt tcttcaccag ccggctgccg tagtcggccg gcacgttcgg cagtggtttg
     4921 ctgagagacg gcaccgcgac gatcgcgaag ttggcggcaa acagtgccat cgcatcgtcc
     4981 gtggccagcc aatccatcag cttctgcgcg gccgcgaggt tcttggtccc cttgtgtatg
     5041 ccgctcgcct ccaggtccca acccaggcct tccttcggga acaccagttc gatcggcttg
     5101 ccttgcgctt tctcgcggtt ggcgcggtac tcgaaggaga taccgaccac gtattcgccg
     5161 ttggcggctg ccgcacacgg acgcgagccc gagtgcatgt attgcgcgat gttctcgtgc
     5221 aagccgtcca tgtacttcca gccctcggcc tcgccccaca actgcagcca cgccgacacg
     5281 tcgaagaagc cggtgccgct cgacgccgga tgcggcatca cgatctggcc cttgtagacg
     5341 ggcttcagca ggtcgcgcca cgtctccggc ttcgggatgt tgcgcttggc ggcctcggca
     5401 gtgttgaagc agatcgtcgc gccccacacg tccatgccga cccaggtcgg cggattcttg
     5461 ctgtcgcgat actgcggcgc gatccggttc agccctgccg gcgcataggg aaccagcatg
     5521 ccttcggtgt cgaagatcgc catgctggtg gcggccacgc ccatcacgag gtcggccttc
     5581 gggttcgctt tctccgccag cagcttggcg gtgatcacgc cggtggagtc gcgtacccag
     5641 acgatctcga tgttcggatt cgccttctcg aagccggcct gataggcctt cagctggtcc
     5701 gtctccagcg ccgtgtagac ggtgagctgc gtcttctgcg ccaacgcgcc gaaggtgaag
     5761 agtgaaccga tcaggccggc ggccaggccg accgcgacac gacgcaatgc catgggattc
     5821 ctcctggatg gaacgaacag caatgtgcgc ccgcggcggg cggcacggac aagatcagat
     5881 tactcgccgc agttgacgcg gcagtgaccg cggcagcacc ttgcgccgac ggctcgccac
     5941 gcccctgcag gcgcgcggca gtctacgcag ccgggttttt cgttgtcaat tgtcttttgc
     6001 agattgtaga caatcaaccg ccgaggccct agactgcgcg ccatgccttc gcgctcgcca
     6061 gcagcaccgc cgtccggcgg caacgtcgtc gcccatccga cgatcgcgct gttgcgcacg
     6121 cggtcccttg cctccgccgt acagcaggag atcgaacgga tgatcctggc cggagaactc
     6181 gcgccgggaa ccaagctgac cgaggcatgg ctgtcggaga agctcggcgt gtcgcgcgga
     6241 ccactgcgcg aggcgtttcg catgctcgag gaagccggtc tcgtccggca ggaaaagaat
     6301 cgtggcgcgt tcgtgcgcga cattccggtc gaggaggcgg tcgagatctt cgacctgcgg
     6361 gcggtgatgg acgaactggt cgggcgcacg gtcgcgacca ctgtgacgcc cgagcagggg
     6421 cgacagctga agacgctggt cgagcagatg gaacaggccg cacgcgccag cgacgcggac
     6481 gcctatcacc tgctgaacct gcagttccat gaccagctcg tggagtatgc cggcaaccgc
     6541 aagatggccg cgatctaccg caagctggtc aaggagctgg cgctgttccg gcgccgcaat
     6601 ctcgcgcagc agcaagcgct gccgcagtct gtcgcggagc accgccagat cctgaaggcg
     6661 atccagtcgg gcgacgcgga ggccgcgggg cgcgcgatgt tcgcgcacgt gatcgaaagc
     6721 aagcaacgca cgctgcgcgc cgaatcggag cgccgtccgc cggcgcgcgt gcgcgcggtg
     6781 tcgagacgag gataggagcg catgctggaa gtcaacggcc gccgctacgc ggctccaagg
     6841 cagccgatcg tggtgatctg cgtggacggc tgcgaaccgg actacatcgc gcagtccgtc
     6901 gccgccggcg cgatgccgac gatgaagcgc tggctggccg agggcacggc gctgatcgcc
     6961 gacagcgtgg tgccgagctt caccaatccg aacaatctgt cgatcgtcac cggtgcgcca
     7021 ccgtcggtgc acggcatttg cggaaatttc ctgtatgacc cggaggcgaa tgccgaggtc
     7081 atgatgaacg acccgaagtg gctgcgtgcg ccgacgctgc tggcttgtct ggccgacgcg
     7141 gggcactcgg tcgccgtcgt caccgccaag gacaagctgc ggcagctgct cggccaccgg
     7201 ttgcgcggaa tctgcttctc cgcggagaag gcggatcagg catccgttgc cgtcaacggg
     7261 atcgacgcgg tgccgtcact ggtcggtatg ccggtgccgg gcgtgtacag cgcggaactc
     7321 tccgaattcg tgttcgccgc aggcgtgcgg ctgctcgaga ctcgccggcc cgacgtgatg
     7381 tacctgtcga ccaccgacta tatccagcac aagcatgcgc cgggcgatgc ggttgccaac
     7441 gccttctacg cgatgatgga ccgctacctt gcgcgtttgg atgcgctcgg cgccgtcgtg
     7501 gtgttgacgg ccgatcacgg catgaacgcg aaaacgtggc tcgacgccag cccgaacgtg
     7561 atctacctgc aggacgcgat cgacggctgg atcggctcgc agcgcgcgcg cgtgatcctg
     7621 ccaatcaccg atccttacgt cgtccatcac ggggcgctcg gttcgttcgc caccgtctac
     7681 ctgcctgccg acgcgttatc gccggtggcc gaccgggtca gcgcgacgcc gggcatcgag
     7741 atggtgctga cgcgtacgca ggcggccgat cgcttcgagc tgccggccga ccgtatcggc
     7801 gacctcgtcg tcgtctcgga gcgcagcgtc gtgctcggca cgtcgaagtc ccgccacgac
     7861 ctgtcgcagc tcgagatgcc gctgcgctcg cacggcggca tctccgaaca gcgcgtcccg
     7921 ctgatcgtca accgccgcat cgacggtctg gacgggaggc ggcgctggcg caacttcgat
     7981 gccttcgatc tcgcactgaa ccgcgcgcaa tgaacgctcc cctgcccatc aagcacgaaa
     8041 gtctgaggat cgccggccgc aagttcgaag gccatggcca cttcgaggtc cgctacccct
     8101 atacgaacga ggtgatcgcg accgtcgcca aggcctccgt cgaagacgtg cgccgcgcgt
     8161 tgtcgatcgc gcgcggtttc cgcagcaagc tcagccgcca cgaccgctac cggatcctga
     8221 tgcgcaccgg cgaaatcatc gccgcgcgca aggacgagat cgcgcgtggc atcacgctcg
     8281 agtcgggtct gtgcctgaag gattcgctgt atgaggtcgg acgcgccagc gacgtgctgc
     8341 tgttcgcggc caatcaggcg ttggtcgacg acggccaggt gttttcctgc gacctgaccc
     8401 atcacggcaa gagccgcaag gtctacacgc tgcgcgaacc gctgctcggg gcgatcgcgg
     8461 cgatcacgcc gttcaaccat ccgctgaacc aggtgatcca caaagtcgca cccgcagttg
     8521 cgaccaacaa ccgcgtggtg ctgaagccga gcgagaagac gccgctcgcg gcgttcatac
     8581 tcgccgatgc gctgtacgag gcgggcttgc ccccggaaat gctcagcgtc gtcaccggcg
     8641 acccgcgcga gatcgccgac gaactgctga ccagtcccga tgtcgacctg gtgacgttta
     8701 ccggcggcgt gccgatcggc aagtacatcg ccgccaaggc cgtctacaag cggcaggtgc
     8761 tcgaactcgg cggcaacgac ccgctcatcg tgatggaaga cgcggacatc gaggaggcgg
     8821 cgacgctggc ggcgagcggg tcgtacaaga actccgggca gcgctgcacg gccgtcaagc
     8881 ggatgctcgt gcacgagaag gttgcagacc gcttcgtcga attgctggtc gcgaagacaa
     8941 aggcagtcag gttcggcgat ccgctcgaca tggcgaccga catgggcacc gtgatcgacg
     9001 aagcggcggc gcaggatttc gaggcgcgag tgaacgacgc ggtcgctcgc ggcgcgcggg
     9061 tactcgtcgg gcacgagcgc cgcggggcgc tgtatccgcc gaccgtgatc gaccaggtga
     9121 cgccagacat gaacgtggtg aagaccgaga ccttcggtcc ggtttctccg gtcatccgaa
     9181 ttcgcgatat cgatgaggcg attcgcgtcg caaactccac cgcgtacgga ctgtcctccg
     9241 ccgtctgtac caaccggctt gactacatca cgcgcttcat cgctgaattg aacgtcggca
     9301 cggtcaacgt gcgcgaagtg ccgggctacc ggctcgaact cactcccttc ggcggtatca
     9361 aggactcggg gctgggctac aaggagggcg tgctggaggc gatgaagagt ttcaccaacg
     9421 tcaagaccta ttcgcttccc tggtgacctg gctgaaatga agtgcaagcg ctgctgaacc
     9481 tgctcgccgc cgtcgccctg ctggtgtggg gcacgcacat cgtgcgcacc ggcgtgctgc
     9541 gtctgctggg cgcaaatctg cggcaggtgc tgcgcggctc ctccgatcgg cgctacatgg
     9601 cctttctggc gggcatcggc gtcacggggc ttctgcagag ctcgaccgcg accgcgctgc
     9661 tggtgtcgag cttcgtatcg cagggcgtga tcgcaacctc ggccgcgctc gccgtgatgc
     9721 tgggcgcaga tgtcggcacc gcgctgatgg tgcaggtgtt ctcgctcaac ctgtcctggc
     9781 tggcgccgct ggcgatcatc gccggcgtga tcgtgtacct gtcgaagaag aacgagcacg
     9841 ccggccagat cggcaaggtc gtgctgggtc tcggcgtgat gatcctcgcg ctgcagctga
     9901 tcgtgcaggc gaccgcgcca ctggcgcagg ccgccggcgt caaggtcctg ttcgcgacgc
     9961 tgaccggcga cgtgctgctc gacgtgctgg tcggagcgct gttcgcgata gcgttctact
    10021 caagcctggc cgtcgtgctg ctgaccgcca cgctgacggc gtcgcacgtg ctgtcgctgc
    10081 cggtcgcact ggcgatcgtg ctgggtgcga acctgggcgg cgggctgatc gcgctgctga
    10141 cgacgttgcg cgccggaccc atcgcgcgtc gcgtgccgct cggcaacctg ctgttcaagc
    10201 tggcgggctg cgcgatcgcg attccgctgc tgtcgttcgg tctcgagtgg ctggcgaaac
    10261 tcgacggcga tccgaaggcg atcgtggtca atgcgcacct ggcgttcaac ctgatgctgg
    10321 cgctgctttt catccagttc gtcggccgca gcgcgcaggc ggtcgagcga ctgctgccgg
    10381 cgccgcacgc ggccgacgcg ccgggcacgc ccaagcatct cgacccggtc gcgctgtcga
    10441 cgccgccgct ggcgatcggc aatgcggcgc gcgaggcgat ccggctcgcg gacgtggtgc
    10501 aggacatgct cgacgggctt ctgcgcgtgc tcaagagcaa cgacttccag ctcgcggaaa
    10561 ggctgtgcgc gcgcgacgac gaagtagacc ggctgtacac cgcgatcaag ctgtacctga
    10621 cgcagatcag ccgcgaggcg ctggaagcgg cggagtcgaa acgctgggcc gacatcatcc
    10681 agttcgcgat caacatggaa cacgccggtg acatcatcga gcgtgtgctg gtcgacatcg
    10741 cggagaagaa gatcgcgcac gggctgtcgt tctccgacgc cggtatgcgg gagatcgagg
    10801 atctgcacgc gcggctgctc ggcaacctga agctggcagt ggcggtgttc ctgcacggag
    10861 acctgaagag cgcgcaacag ctgatcgcgg aaaaggtcag cttccgcgag ctcgagatgc
    10921 gttacgccga caaccacctg cagcgcctga gcggcaactc gccccagtcg gtcgagacca
    10981 gttcgctgca cctcgacctg ctgtccgact tcaagcgcat caactcgctg ttctgcgcga
    11041 tggcctaccc catcctcgaa caggccggcg tgctctcgag gacgcgcctt gtcggcgagc
    11101 agccggccct ctttgccgcc gacgagcatc ggcacgcatg aaggattggc accgctacag
    11161 cggcacgatc agcgaagccg agcggcgcga gttcgtccgc ttcatgcacg aattgatcgg
    11221 cgccgccttc gcggtgatcc ggccgttctt cctcagcggc acagccgtga tgacgaaggg
    11281 cgacgccagc ccggtgacgc tggctgatcg cggcgcagag gaggcaatgc gcacactgat
    11341 cgaggtgcgc tatccggagc acggaatact cggcgaggag ttcggggcca agatgggacg
    11401 cggctaccgc tgggtgctcg acccggtcga cggtacgcgc gcgttcatca ccaactgctt
    11461 cctgttcggc acgctgatcg cgctggagcg cgacaacggc gccggcttca agccgatcct
    11521 cggcgcgctc gcgcatccgg cggccggcgt ggcactgatc ggccactcgg ggcagacgag
    11581 cctgtacgcg agcgacggtt cgagccgcgc cgttcatgtt cggtcatgcc ggcaactcga
    11641 aaacgccacc gtgctcgcaa ccacccactg gtccaccggc gagcagcgcg ccggcgcagc
    11701 ggtcgaaaac gtgatccggc gcgcgaagct gtaccgcacc tggggcgact gcttcggtta
    11761 cttcgcgctg tgcacggccg gcgccgacgt gatgctcgac ccaaccctcg catactggga
    11821 tgtcgcggcg atcgtgccgg tggtcgaggg cgcgggcggc cgcgtcacga gctggcgcgg
    11881 cggcgatccc ctcgatgatc cgtcgctgct cgccacggcc ggcggattgc aggacgagat
    11941 cctgcagatc atccaggccg cagcctgaac atcgccgcgc caacgctgcc ctccttcgct
    12001 cgcaacccag cgcctttcca aggcttgcgc agcggccccg gcgggccgct cgagacaggc
    12061 tgaccgccta gaagggaatg tcgtcgtcca tctcggcgat gttctgggcc ggcttggccg
    12121 cgggcttggc ggcaggtcgg gcgggcggcg cggactcttc gatgtcgcct cctcccatcc
    12181 cctcacgcga accaagcatc tgcatgttct cggcaacgat ctcggtcgtt tgcttctccg
    12241 cgccttcctt gtcggtccac ttgcgcgttc gcaggcggcc ctcgacatac actgaacggc
    12301 ccttgcgcag gtactcgccg gcgatctcgg ccaggcgccc gaagaagacg acgcggtgcc
    12361 actcggtctc ttcctgctgc tgaccggagg aatccttgta gcggcgcgtg gtggcaaccg
    12421 acacgttggc aatcgcggcg ccgtcggcgc tgtaccgcgt ctccgggtcg cggccgagat
    12481 tgccgatcag aatgaccttg tttaccgatg ccatcgcgcg tcctccctcg cctcgaggcg
    12541 gccatcatac cgactggcac gcggggtcgc ggcggctcgc gtcgcgcgtg gcgccgccgc
    12601 tatagtgtcg agttcggctt cgacctcgcc atcgcatgga tgccatcaag gttcgcggcg
    12661 cgcgaacgca caacctgaag aacatcagcc ttgatctacc gcgcaagcgg ctgatcgtac
    12721 tgacgggcct gtccggatcg ggcaagagtt cgcttgcgtt cgacacgctc tatgccgaag
    12781 gacagcggcg ctacgtcgag tccttgtccg catacgcgcg gcaattcctg cagctcatgg
    12841 agaagcccga cgtcgattcg atcgagggcc tgagccccgc gatcgcgatc gagcagaagg
    12901 cagcatcgca caacccgcgc tccaccgtcg gcaccgtgac cgaggttcac gactacctgc
    12961 ggctgctcta tgcgcgcatc ggcacgccat actgcccgga ccatccggag catcctctcg
    13021 ccgcgcagtc ggtggcgcag atggtcgaca gcgcgctcgc gctgccggcc gacacgcggc
    13081 tggtggtgct ggcgccgatc gtgcgcgagc gcaagggcga gttcgaggac ctgttcgacg
    13141 acctgcagac acagggcttc gtgcgctttc gcgtcgacgg tgcgatcgtc gaaccgaatg
    13201 acctgccgca tctggcgaag aacgaaaagc actcgatcga tgtcgtcgtc gaccgcctga
    13261 aggtgcggcc tgaagccaag cagcggctgg cggaatcgtt cgagactgcg cttcgaatcg
    13321 cggcgggccg cgcgctgctg ctcgacaccg acaacggcac cgagacggtg ttctcgaacc
    13381 ggttcgcctg cccgatctgc ggctacgcgc tggcggaatt ggaaccacgg ctgttctcgt
    13441 tcaacaaccc ggtcggcgcc tgcccgtcgt gcgacggcct gggcacggtc gagtacttcg
    13501 acgagcagcg cgtcgtcgtg catccggagc tgacgatcgc cagcggcgcg atcaagggtt
    13561 gggatgcgcg caatcagtac tacttccaga tgctggccgc gctggcaaaa cacgcacaag
    13621 tcgacatcga cctgccctgg ggagcgctgc ccgagtcatt ccgcacgcag gtgttgcttg
    13681 gcagcggcac cgaggtgctg ccgttcgcct acaccaacga caagggacgc cggatcgtgc
    13741 gccaccacgc tttcgagggc gtgctgccga acatggagcg gcgctggcgc gaaacggatt
    13801 ctgccgcggt gcgcgaggaa ctcgccaagt gccgcgcggt gaagccctgc cccgcgtgcg
    13861 acggcacgcg cctgaagcgc gaggcgcgct tcgtgttcgt cggcaatggt ccgcagaagc
    13921 gagcgatctt tgaaatcgag cgcgcgccgc ttgcgcagac gctcgcgtat ttcgacgcgc
    13981 tggaactgga aggagcgaag cgcgagatcg gcgcgcgcat cgtgcgcgag atcggcgcgc
    14041 ggctgcggtt cctgaacgac gtcgggctca cgtacctgac gctcgaccgc tccgcggaca
    14101 cgttgagcgg cggcgaatcg cagcgcatcc ggctggcgtc gcagatcggg tcgggcctga
    14161 ccggcgtgat gtacgtactg gatgaaccgt cgatcggcct gcaccagcgc gacaacgaac
    14221 ggctgatcgc cacgctgaaa cacctgcgcg atctgggcaa caccgtgctg gtggtggagc
    14281 acgacgagga cgcgattcgt gccgccgacc acgtggtcga catggggccg ggggcaggcg
    14341 agcacggcgg cgaggtggtg gcgcagggca cgccgtccga catcgagaat tctccggcct
    14401 ctctgaccgg cgcttatctc gcccgccgtc gcgcgatcgc agtgccggcc gcgcgcgtgg
    14461 cgccgcgact gcactggctg aggctcgcag gggcgcacgg caacaacctg aagcacgtgg
    14521 atctcgaact gccaatgggg ctcctcgtgt gcgtgaccgg cgtgtcaggc tcgggcaagt
    14581 cgacgctgat caacgacacc ctggcggcca tcgtcgcgcg gcacctctac gggtctgccg
    14641 tgctggccgc gccgtacgaa tcggtcgagg gactggagca tttcgacaag atcgtgatgg
    14701 tcgaccaaag cccgatcggc cgcacgccgc gcagcaaccc ggcgacctac acgggactgt
    14761 tcaccccgat tcgggatctg ttcgccggca cgccgaccgc acgcgagcgg ggctacgagg
    14821 ccggccgctt cagtttcaac gtcaagggcg gacgctgcga aacctgccag ggcgacggcc
    14881 tgatcaaggt cgagatgcac ttcctccccg atgtctacgt gccgtgcgaa acctgccacg
    14941 gccagcgcta caaccgcgag accctggaag tgctgtacaa gggccgcaat atcgccgagg
    15001 tgctcgacct gaccgtcgag caggcagccc agttcttcag tgcggtaccg gtgctggcga
    15061 agaaactgca gacgctcgcc gacgtggggc tcggctacat ccgcctcggc cagagcgcaa
    15121 ccacgctgtc gggcggcgag gcgcagcggg taaagctttc cctcgaactt tcgaagcgcg
    15181 acaccggcag cacgctgttc atactcgacg agccgaccac gggactgcac tttcacgaca
    15241 tcgaactgct gttgcacgtg ctgcatcagt tgcgcgatgc cggcaacaca gtggtcgtga
    15301 tcgaacataa cctcgacgtg atcaagaccg cggactgggt catcgacctc ggaccggaag
    15361 gcggcgaagg cggcggccgc gtcgtcgccg ccggcacgcc agaggcgatt gccgcgcact
    15421 cgggcagcca caccggtcgc tttctcgcgc gtgtgctgac gcgcgccgag gagcgcgaga
    15481 cgcgccgcgc gccggcgcgc agacactccg gcggacttgc agtcagttag tctgcatttg
    15541 atcgatacca aatggccccg gcaggacgaa cttcgatggg cgccgtctcg tgctggtgcc
    15601 agtaccgaag cggaccccag gtgcaagagg tgaaaggcag cgacgcacgc gtgagattcg
    15661 cggtcgcgtg cgcagcgcgt cgcggctcgc cacgcgatca gatccgcgct acgccgtctt
    15721 gatctgcagc agccgctcct cggcgagttc gagcgcctcg ggcgcgccgc gcagcacgac
    15781 gacgtcaccg acctgcagca ccatttcgtc gctcggatcg gcgccgcgga tgccgcgccg
    15841 gcgcaccgcg gttacctcga cgccgatcga cctgaggccg agcgaaccca acatagcgcc
    15901 gaccgcggcg gcgccggcct cgatcggcag cgaatgcagg cgcacgtgcg attcgtcctc
    15961 gaactcggcc ggatcgtcgg cgccatggaa gtagccgcgc agcagccggt agcgtgagtc
    16021 gcgcgcctcg cgcacacgcc tgaccacgcg ctgcagcggc acgccgagca gcaccagcgc
    16081 gtgtgagccg agcatcaggc tgccctcgaa gatctcgggg accacctcgg tcgcgccggc
    16141 gcgcaaaagt cggtccagat cggcatcgtc ctgcgtgcgc acgatgatcg gcagagcggg
    16201 ccgcgcctcg tgcgcgaagt ggatcacctt cagcgctgaa gccgagttgt tgtagctcac
    16261 gaccagcgcg gcggctcgca tcaggcctgc ggcgatcagc gtttcgcgcc ttcccgcgtc
    16321 gccgaagacc accgagtcgc cggcggccgc cgcctcgcgc acgcggtccg ggtcgaggtc
    16381 gagggcgacg tagccgaggt tctcctgctc gagcatcttg gccagatgct ggccgctgcg
    16441 cccgtagccg cagatgatca catgacgctc ggtggccatc gagcgcgtag ccacctgagt
    16501 cagttgcagg gcacgcagca tccattcggc gctggcaaag cgcattgcga tgcggtcgct
    16561 gtaatggacg agaaaaggcg ctgcgagcat cgacagcagc atcgccgcga gcacgatctg
    16621 tttcacgtct ggcgccagca gcgcacccga ttccgaaagc agcacgaagc cgaattcgcc
    16681 ggcggtagcc agcgccagcc cggtgcggat cgccgtgcca gggccgacgc gaaacatccg
    16741 cgccagcgcg acgattagcc cgcacttgaa cgccgaaggt atcagcagcg cgagcaccac
    16801 ccagccgatg ttctcgatca cgatgcggac gttcagcagc atgccgatcg tgacgaagaa
    16861 cagccccagc agcacgtcgc gaaacggctt gatgtcctcg gccacctggt ggcggtactc
    16921 ggtctcggaa atcagcatgc cggcaacgaa cgctccgagc gccagcgaca gccctgcatg
    16981 ctcggtcagc caggccagcc cgagcacgat cagcagcagg ttcagcgtga acaattcatg
    17041 cgacttgcgc cgcgcgacca gatgcaacca gtgccggatc agccattgcc cgcccacaag
    17101 tagcagcccc agcaggatcg ccgccttggc cgcggcccac atcagcgccg cgcccagctt
    17161 gtcgggttcg gtggcgagcg cggggatcac gaccagcagc ggcacgacgg cccggtcctg
    17221 gaacaacagc acgccgacga tgcgcttgcc gtgctctgtc tccagttcga gccgatcgac
    17281 cagcagtttg acgatgatcg cggtcgacga catcgcgagc gtggcgccga gcacgaaact
    17341 cgccgcgcgc gtgaggccca agtgcgaacc catcatccag cccgcggccg tgacgagcac
    17401 ggtcgtcgcc accacctgcg ctagcccgag cccgaagacg atccgccgca tgctcttgag
    17461 cttcggcagc gaaaactcga gcccgatcga gaacatgagg aatacgacgc cgaactcggc
    17521 aaggtagcgc gcgtcggccg agtcgggtac gaccccgagc gcgtgcggac cgatcaggat
    17581 gccgacgacc agataggcaa gcgtcggcgg caggtcgagc aggcgaaacg ccaccactcc
    17641 agccacggaa gcggcgagca ggaacagcgt cagttccaga gcggtcggca tggcgcgcta
    17701 tgcgacggac cggaaggccg ccatcagccg ttcggctaca gcaattcctg tgccagcgtc
    17761 atggtcgcga gagtatcatc gaccgcgaat tccccgccgc aatttccccc gccccgtcac
    17821 ctgatggaag acgcgcgcac ccgaccccgt gccccagcgc tcgaacttgc gcgtgaggtg
    17881 ctggcgatcg agtcacagtc ggtggccgcc ctcgcggacc gactcgatgg ccgattcctg
    17941 cgcgcggtcg acctgctgct cgcctgccgc gggcggatcg tcgtgtcggg tgtggggaag
    18001 agcggtcata tcggccgcaa gatcgccgcc acgctggcgt cgaccgggac cccggcactg
    18061 ttcgtgcacg cggccgaggc cgcgcacggc gacctcggca tgatcacgcc ggccgatgtg
    18121 ttgatcgcgc tgtcctactc gggtgagact gcggaactcc tgacgatcgt gcctctggtc
    18181 aagctcgaag gcacgccgat gatcgcgatc acgggcaatc ccgaatcgtc actggcgcgg
    18241 catgcggatg ttcacctgga cgtgcgcgtc gacaaggagg cgtgtccgct gaatctcgcg
    18301 ccaacctctt cgacgacagc gacccttgcg ctcggcgacg cgctggcaat cgcctgcatg
    18361 gaggcacgcg gattcgggcc gtccgatttc gcacgctcgc atcccggcgg cgcgctcggc
    18421 cggcggttac tcacgcgagt ggcggacatc atgcgcaccg gcgatgcggt gccgtcggtg
    18481 ccgatcgacg ccagcgtgct cgacgccgtg gccgagatcg gcaggaagcg cctcggcatg
    18541 actgcgatcc tggcgcgcga cggttcgctg gcgggcatct tcaccgaggg cgatttgcgc
    18601 cgactgatcg agcgcgtcgg cgacgtgcgc gccctgcgcg tggcagaggt tatgacgcgc
    18661 aagccgctga cggttccctc cgccatgctg gccgcggaag ctgcgcgaat cctcgatgcg
    18721 agcctgcgca accagctgct ggtcatcgat gacggccggc tggtcggcgc gctgcacatg
    18781 cacgacctga ccgccgcgaa ggtgctttga tgcccgatga cgccgccgcg cgcgccgcga
    18841 acgtgcgcct gctgatcctc gatgtcgatg gcgtgctgac cgacggacgc ctctacttcg
    18901 gaccgcaggg agaagcactg aagatcttcg acgtccgcga cggccacggc atcaagctgc
    18961 tgctcgaagc aggcatcgaa gtggccatcc tgtcgtcgcg ccgttccgag gcggtcgagg
    19021 tacgcgcgcg tgaactcggc atcgcgcacg tgctgcaggg cgaagccgac aaacgcgccg
    19081 gcctgcagcg cctgctcgcc gccctgcggc tgtcgccgga gcaatgcgcg ttcatcggcg
    19141 atgactggcc cgacctggcg gtgctgaaac gggtcggcct ggcggcgacc gtcgccgacg
    19201 cggcgcccga ggtgcgcgac gccgcgcatt ggatcgcacc cgccggtggc ggccgcggtg
    19261 ccgtgcggca actcgcggaa ctcatcctgc gcgcgcaagg caagttcgac gccatgctcg
    19321 cgcggcattc gaggctgggc ggacatgctt gagcggaaga gcctgcgcgg cgtgaccgcg
    19381 tgcccatccg tcgctcccgt tccgcggcgc cggtccggcc gcccaaagag cggtgcgccg
    19441 aatggggcgc gcgaacggca cgcgcgttgg gcggcgatgc gtacgtctcg tgcggtttct
    19501 gatgcgtgac cgcatcacga tgatcatcgc gatcgcgctg ctggcgatcg tcaccgcgac
    19561 cagctactgg tactcgcgtg tgatgcgcaa gccggagagc gccgaaccgc cgcggcccgg
    19621 aacgccagac atcgtggtcg atcgcgtggt cctgacgcag ttcgacgagc aggggcgcgc
    19681 ccgcaacaag ctgctcggcg agaggctgcg ccatttcgcc gaaaacgacg atatcgagat
    19741 cactgcgccc cgcctcgtca gcctgcggcc ggatcagccg cgcgtcgagg cgcgcgcgaa
    19801 cgctgcgcgg gtcgagaacg cggcagagcg cctgcatctg aacggcgacg tcgtgatcac
    19861 gcgcgccgca ttcgccgggc agccggagat gcgcatgacg accgagtacc tgctggtgct
    19921 gcccgacgag gatcgctacc ggacagatcg cccggtgaag ctcacgcgcg gcgcttcgat
    19981 gatccgcgcc aacgcgatgg atttcgacaa catcgcacgc atcgtgatac tgaccggtgc
    20041 ggtccacagc gtgatcgaac cgcgacccgc ggcaaggcga tagccgatgc gagcttcatt
    20101 cctgcgttgg cttctgacgg cgcttgcgct ggcgagcgcg ccggcgtggg ccgatcgcgc
    20161 cgaccgtgac aagccgacgc agatcgacgc cgatgcgtgg cgcggcgaag agttgaaaca
    20221 ggtcgtcgtc tacagcggca aggtcgtgct gaccaagggc acgctgcgca tcaccggcga
    20281 ccgactcgaa ctgcgcgagg acccggaggg ctaccagtat gcggtcgtaa cagcccgacc
    20341 gggcgagctc gcgaccttcc gccagcggcg cgacccgacc cagcccgggg tcgaggaatt
    20401 catcgaggcg gaagccgaac ggctcgaata cgacggcaag gcggagacga taaagctcag
    20461 cggccgcgcg caggtcagac gcatcgagag cggcgagcca cgcgacgagt tccgcggcgg
    20521 cgtgatcacc tacaacatgc gcgaatcgcg ttacagcggc cagggcggcg aggccgccga
    20581 aggcggacgc acccgcacga tcatcgcgcc gcgttcggca gcgccggccg cgccgccgac
    20641 gccgctggca cctgcgcgaa cgctcgagcc ggaacgccgg aagtgaacga caccgcctcg
    20701 acgtccgccc agccgaccat gcgcggatcc ctggcgcgcg gccgactcgt ggtcgagcat
    20761 ctgcgaaagc aatacggaac gcgggtggtc gtcaaggatg tttcgctcac cgtcgaggca
    20821 ggcgaggtcg tgggcctgct cggaccgaat ggcgccggca agacgacctg cttctacatg
    20881 gtggtcggcc tcgttcccat ggacggcgga acgatctcgc tggacgacac gtcgattgcc
    20941 cacctgccga tccatcggcg cgcccgcctg gggctgtcgt acctgccaca ggaggcatcg
    21001 gtgttccgcc gcctgacggt ggaagaaaac gtgcgcgcgg tgctcgaact gcaggttgac
    21061 gccaaccgca agccgctcgc gcccgcccgc gtgcgggaac agctcgatgc gctgctcgac
    21121 gagctgcaga ttgcgcacct gcgtaccaac accgcgcaga gcctgtcggg cggcgagcgc
    21181 cgccgcgtcg agatcgcgcg ggcgctggcc gcgcggccgc gctttattct gctcgacgag
    21241 ccgttcgcgg gcgtcgatcc gatcgcagtg atcgagatcc agcgcatcgt gcgtttcctc
    21301 aaggatcggg gcatcggcgt actgatcacc gatcacaatg tgcgcgagac acttggcatc
    21361 tgcgaccacg cgtacatcat caacgagggt caggtgctcg ccgcgggcgc gccgcacgag
    21421 atagtcgaga acgactcggt ccgccgcgtc tacctcggcg agcacttccg gatgtagtcg
    21481 gcgtcggatg aagccgagcc tgcaactcaa gcttagccag cacctggcgc tgacgccgca
    21541 attgcagcag tcgatccggt tgctgcagtt gtcgacgctc gagctgaatc aggagatcga
    21601 gcaggcgctc accgacaacc cactgctgga gcgcgacgac gacccgctcg cgacgtcgat
    21661 gcgtatctcg tccgacggcg cgatcgtcga caagacgccg ccggtcgagg cgggcgatgc
    21721 ggcgcaggcg acgccgacga cggaggccaa cggcgccgag cacgcggaag gcgaagccgc
    21781 gctgcacgac tggggttcgg cgactggcgg cccgaccgac gatgacgact ccagtccgct
    21841 atcgtgggcc gccacgcgca cgacgctggc cgagcatctg aaggcgcagc ttgcgacaac
    21901 gcaggtttca gcccgcgatc gcgttctggt cgagctgctg atcgaggcgc tcgatgaagg
    21961 cggctacctg acctccacgc tcgaagaagt gcggcagatg tgcccacccg acgccgggat
    22021 cgagttcgaa gaactcacgg ctgcgctgaa gttgctgcag agcttcgatc ctcccggagt
    22081 cggcgcgcgc acgccgtccg aatgcctgct gatccagctc gaactgcttt cccggcacgc
    22141 cacgccaccg tgctcggcgc aagtccttca acatgcaaag gcgatagtta gcgaacactt
    22201 gccgttgttg gcgaacaggg attacgcgaa gctgcgtcga ctgctcggcg cgagcgatga
    22261 ggatctgcgt accgcgcatc aggtcattcg tggactgaat ccgcatccag gggtaggctt
    22321 tggcgcagag acgatcgact acgtggtgcc ggacatcttt gtccggcgta tcaaggggcg
    22381 atgggtcgcg cagttgaatc ccgacgtcat gccgcgattg cgcgtgaatc agcactatgc
    22441 gtcgattgtc aaacgcgaca gaggcaagga aggacaggcc ccttggtcgg cacggttgca
    22501 ggaagcgcgc tggttgatac gcaacatcca gcagcggttc gacaccatcc tccgtgtcgc
    22561 ccaggcgatc gtggaccgcc agcacaattt cttcacgcac ggcgccatcg caatgcggcc
    22621 cctggtgctg cgcgagatcg ccagcaccgt aaacctgcac gaatcgaccg tttcccgggt
    22681 gacggcaagc aagtacctgt cgactccgtt cggcgtgttc gaactgaaat acttcttcgg
    22741 cagccatgtg gcgaccgaaa ccggtggcgc ggcatcgtcg acagcgatcc gcgccttgat
    22801 caagcaactg ataggagccg agaacccgaa ggaaccgctg tctgataacc gaatcgcaga
    22861 actgctcggc gaacagggca tcgtggtcgc tcggcgcacc gttgcgaaat accgcgaggc
    22921 gctgaggatt ccgccggtgg caatgcgaaa ggtcctgtag cgacgccgcg agcggcgtcg
    22981 atccttcaat cctgccggca cgcgcttcgg cgcgtttccg gcgcaagcgt aagggtgctg
    23041 catgaacctc atcatccacg gtcatcacgt cgaagtgacc ccggcactgc gcggctacgt
    23101 cgaaggcaag atggaacgga tccggcgcca tttcgatcac ctgattgaag ccgacgtggt
    23161 gctctcggtg gaggacaagc tcaggcagcg tgcggagatc accctgcggg tgcgcggcaa
    23221 cagcctgcat gcggaggcaa cggaagagga catgtatgca gcgatcgacg gtctgatgga
    23281 caagctcgac cgccaagtgc tacgccacaa ggaccgggtc cggaaccacg tgcgcgatcc
    23341 gctcaagcac cagtccgcac aacagcagca gtaagccggc tggatatggc caatgcggat
    23401 ctgacccgca gaccggatcc gctgttgctg caacgcaaca actcgccgaa gtcccggctc
    23461 gggccgaaga caatgatcaa ttccgcccca ccacggcgcc tatactgcgc cgcgtctcga
    23521 ctgccaatcc gcggaacgca atcgcctgcc ccggtgatcg gcctgacgcc gaacctcccg
    23581 ccggaacgac ccgctgaccg agcgatgaac gcgaagtccg gccggaggcg tccaatccgg
    23641 gttgtacgcg gcgcgtagtc ttcatttcag ctgtcgcgcg tccgccgcca aggtttcgat
    23701 attcgaatga acctgatctc tcgcctgatg ccgccgtcga acgtcctcct cgggcttgcg
    23761 gcatctagca agaagcgtgt gttcgaacaa gccggcctgc tgttcgagaa caaccagggc
    23821 atcggccgtg cggcggtgtt cgacagcctg tttgcccgtg agcggctcgg ctcgaccggt
    23881 ctcggccaag gcgtggcgat cccgcacggg aggatcaagg gcctgaagga cgcggtggca
    23941 gccttcattc gcctggaaga gcccgtgccg ttcgatgcac ccgacggacg acccgtgcaa
    24001 ctgttgatct tcctgctcgt gcccgaacag gcaacccagc agcacctcga catcctgtcc
    24061 gaactggcgc agatgttgtc ggacaagacc tttcgcgagg ggctgctgac ggccaccgac
    24121 ccgatggccg tgcatgaaat gctggcgtcg tgggagccgg tgcgcccggc tgcctgacgc
    24181 aagcgcggga gcgtcggcta agctcgctgc atgccgacca tccaatccct cttcgaagaa
    24241 aacgccgatc gcctcaggct gcgctggatc gcgggacgca agggcggcga gcgcaagttc
    24301 cagggtagcg gcgcgctgga tgttcccgcc gcggacctgg tcggacatct gaacctgatc
    24361 caccccaatc gactccacgt gctcggcggg cccgaggtcc gatatgtggc gcggatggac
    24421 gctgcacggc gcgctcacta cgcggacgaa ctcgtcacgg gcggcgcgct cggcctgatc
    24481 gtcgcggagg acctgagctt tccccccgac atggaatcgg ccgccgagac cgccgggctg
    24541 gcagtgttct cctcgccgct accggccgcc aacgtgatcg atctgctgcg gatctatttt
    24601 gggaaggtcc tggcggcgca ttgcacgatg cacggtgttt tcatggacgt gctcggaatg
    24661 ggagtactga tctcgggcga gtcgggcgtc ggcaagagcg aactcgggct ggagctgatc
    24721 agccgcggtc acggactggt cgcggacgac gtggtcgatt tcgcccgcgt cgcgccggat
    24781 gcgatcgagg gccgctgccc gtcgttgctg tccaaccttc tcgaggtgcg cgggctcggg
    24841 ctgctcgaca tcaaatcgat cttcggcgaa accgcggtgc gacgaaagat gaagctgcgc
    24901 ctgatcgtcg agttgcgccg gcgcgctgcc gcggagatga tggaacggct gcctctggcc
    24961 ggcacgaccg aggaggtgct cgggctgccg atccacaagg tcgtcatccc ggtggccgct
    25021 gggcgcaacc tggcggtact gctggaggct gcggtccgca acacgatcct gaagctgcgt
    25081 ggcatcgata cgatggcgga tttcctggag cgccagcggc aggcgatgga cgacatatag
    25141 gagtgacggc ggccaagtca acgcggcgaa ccggcaggcc gttgtagcag cgctggcgca
    25201 ggccgatgcc gaacgccaga acacccaccc tcggaggaga agacgatgct caagcaagta
    25261 ctgatcgcgg cgtgcctggt gcttcccgtg gctgccatgg ccgcggatga aaagaaaatg
    25321 accccgcagc agcagaagat gtccatgtgc aacaaggagg ctggcgagaa gaagctcgaa
    25381 ggcgatgcgc gcaagggctt catgaaggaa tgcctgtcgg cgaagccggc cgccaagatg
    25441 acgcagcagg acaagatgaa atcctgcaac aaggatgccg gtgccaagaa cctgaagggc
    25501 gacgagcgca agaagttcat gtccgagtgc ctgaagggct gacgcaaggt cccgaaggac
    25561 gagccgaagg ggcgcggtat cgcgcccctt cttgtttctc gccgccaccg gaccggcgtg
    25621 caacaatggc cgcatgcgtg tggtactggt caccggtatg tccggatcgg gcaagtcggt
    25681 cgcgatccgg ctgctcgagg acttgggcta ctactgcatc gacaacctgc cgtcgcgctt
    25741 cctcggtcag gtctgcgcgt atctgagcga cgccggacac aacgaggtcg ctgtatcggt
    25801 cgatgcacgg accgagatct ccctctcgca gttgccgggg gtgatagacg aactgcgcgc
    25861 gtccggcaac gatgtacgcg tgctcttcct caccgcctcg accgaagcgc tggtgcagcg
    25921 ctattccgag agcagacgcc gccatcccct gtccgaacgg ctcgcgctgg gcacggacgg
    25981 tctcgaacct acgctgaccg aatcgatcga ggccgaacgc gatctgctgg caccactgaa
    26041 ttcgatcggt cacctgatcg ataccagtgc gctgcaggcg aacacgctgc gcacctgggt
    26101 gcggcagttc gccgacagcc cgcgcgcaac gctgacgctg acgtttgaat cgttcgcgta
    26161 caagaacggc atcccggtcg acgccgacct ggtgttcgac gtgcgcaatc tgccaaatcc
    26221 gttctacgac ccgcggctca agcccctgac cggactcgat gagcccgtga tcgcgttcct
    26281 ggcgtcgtcg aagcgggtcg acgagatgat cgaggacatt ggccggtttc tcggccgctg
    26341 gctgcccggt tacgtgcagg actcgcgcca ctacgtgacg gtagcgatcg gctgcaccgg
    26401 cggtcagcac cgctcggtct acgtggtcga gcagctcgct cggcgtttcg caggacaggg
    26461 gcgcgtgctg gtgcgccacc gcgggatcgc gcggcattga cgcgagcgct gccgatgcgg
    26521 ctgccgctgt ttccgctcaa cgccgttctg tttcccggtg gcagcctgcc cctgcgagtg
    26581 ttcgaagcgc ggtatatgga catggtgcgc gattgtctgc ggaactcgtc gcagttcggt
    26641 gtgtgcctga tcacgcgtgg ccgcgaaacc ggcaagcaag ccgagtgcga gccggtcggc
    26701 tgcatggctt ctatcgccgc atgggacatg cagaagctcg gcgtgctgaa catccgcacg
    26761 ctcggtacgc agcggttccg ggtccggcac gccgcggccc aaccggacgg gctgctggtc
    26821 ggtgacatcg aggagattgc ggccgatgtc gacgcaccgc tcgcgcctga gcatcagccg
    26881 tgcgcgacgc tgctggcgcg catcatcgac gatctcaacg cgcagcgcga ggcggcactg
    26941 cgcgaccacc cggacgacgc cgggatgctg gccgcgaacg cgatcgagcg gccccatatg
    27001 ctgcactcct cggtatgggt cggaaacctg ctgtgcgagg ttcttccggt ttcgttgcgc
    27061 gcaaagcaga agctgatgga actggacgac gcgcgcacgc ggctcgacat cgtgcagcga
    27121 ttcctgcggc
//
LOCUS       DBNHBY010000433         6004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_567551, whole genome shotgun sequence.
ACCESSION   DBNHBY010000433 DBNHBY010000000
VERSION     DBNHBY010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_567551"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1053
                     /gene="hslO"
                     /locus_tag="AC16HB_10875"
     CDS             136..1053
                     /gene="hslO"
                     /locus_tag="AC16HB_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR5607337.1"
                     /translation="MSDSLLKFLFRRAPVRGGLVRLDQAWKQMIAFHDYPAPVTRLLG
                     EMTAAVALLATNIKFNGALVLQVHGDGPVKLLVVECQPDVGLRATAKLRADAVIDEAA
                     GLQAMVNAHGRGRCAITLDPRDPQPGRQPYQGVVPLEGDTIAQALESYMRRSEQLDTH
                     LRLAADGEIAAGLLLQKLPREGGRSNVAEDADAWGRALALAGTLGGAELLAQPPDTLA
                     HRLFWQEPLDAYAPITPRFQCTCSRDRIGRMLISLGREEVESIVAEQGRVEVTCDFCN
                     AKRVFDAVDVAQLFATGSAVEIDDTARPH"
     gene            1067..1417
                     /locus_tag="AC16HB_10880"
     CDS             1067..1417
                     /locus_tag="AC16HB_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607338.1"
                     /translation="MQPRWLALSALLLLAACATTLPDGSRIERLPAGATTSTPQLGPQ
                     EAQKLAQLNAQVLAEQDAARAREAREEAWRRAQQQLQLDLYYGTGWWGWHDRWGHRHP
                     RWSFGLGWYGPWPY"
     gene            complement(1439..1741)
                     /gene="ftsB"
                     /locus_tag="AC16HB_10885"
     CDS             complement(1439..1741)
                     /gene="ftsB"
                     /locus_tag="AC16HB_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIR5607339.1"
                     /translation="MRLLALALAVLLAAIQWPLWFGKGGWLRVAELDRQLAAQRAAND
                     ELAARNAQLAAEVASFKAGREAIEERARSQLNMIRNDELFFQFVPVPKADKPAAPQ"
     gene            complement(1748..3031)
                     /gene="eno"
                     /locus_tag="AC16HB_10890"
     CDS             complement(1748..3031)
                     /gene="eno"
                     /locus_tag="AC16HB_10890"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR5607340.1"
                     /translation="MSSIVDLIGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKGRYLGKGVLKAIENINTEIAEAVMGLEANEQAFLDRTLIDLDGTEN
                     KMRLGANATLAVSMAVAKAAAEESGLPLYRYLGGSSAMQMPVPMMNVINGGAHANNNL
                     DLQEFMIVPVGAPSFAEAVRYGAEVFHALKKLIHERDMSTAVGDEGGFAPSVKSHEEA
                     IKLILEAIDKAGYEAGTQVALALDCAANEFHKDGKYVLEGEGLTLDAGGFANLLATWC
                     DKYPILSVEDGMAENDWDGWAQLTKLLGRKVQLVGDDLFVTNTRILREGIARGIANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVVSHRSGETEDSTIADIAVGLNVLQIKTGSM
                     SRSDRIAKYNQLLRIEEDLGDVASYPGRSAFYSFR"
     gene            complement(3053..3358)
                     /locus_tag="AC16HB_10895"
     CDS             complement(3053..3358)
                     /locus_tag="AC16HB_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR5607341.1"
                     /translation="MPAAYVLVEMKLTDPEQYKQYMAAAPATIAAAGGEYLARGGRCE
                     TLEGDWQPARVAVVKFPSLAAAKAWYEGEQYRAVRAKRKGATEYFNMIVVEGVPPTL"
     gene            complement(3369..4223)
                     /gene="kdsA"
                     /locus_tag="AC16HB_10900"
     CDS             complement(3369..4223)
                     /gene="kdsA"
                     /locus_tag="AC16HB_10900"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR5607342.1"
                     /translation="MRLCGFDAGLDRPIFLIAGPCVIESETMALDTAGQLKEITGKLG
                     MPFIYKSSYDKANRSSGKSFRGLGIETGLRILSEVKKRIGVPVLTDVHAEGEIADVVE
                     VVDVIQTPAFLCRQTDFIRACAVSGKPVNIKKGQFLAPHDMKNVIDKAREAAREAGLS
                     DDRFLACERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGIASGGQRE
                     FVPVLARAAVAAGVAGLFMETHPDPAKALSDGPNAVPLHRMAELLATLVELDRVTKQR
                     GFIETNFG"
     gene            complement(4220..5887)
                     /locus_tag="AC16HB_10905"
     CDS             complement(4220..5887)
                     /locus_tag="AC16HB_10905"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5607343.1"
                     /translation="MTRYVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLVKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISAKMRRVNNFTTGQIYESVIKKE
                     RRGEYLGKTVQVIPHITNEIQEFIERAPRAAWDGATDVAIVEIGGTVGDIESLPFVEA
                     ARQMSLRLGRNSCCFVHLTLVPFIPSAGELKTKPTQHSVQKLREIGIYPDVLLCRADR
                     PIPEDERAKISLFSNVPVDSVISVWDADSIYKIPAMLHQQNLDAIVCDKLGLHAKSAD
                     LSAWDALVYALEHPQQEVRIGMVGKYVELSDSYKSLNEALIHAGIHTRTKVTIEYVDA
                     EQFEKPGPVKLDHLDAILVPGGFGRRGTEGKIRAVQYAREHKVPYLGICLGMQLATIE
                     FARNVCGLAGANSTEFDPDTPHPVVALITEWLDRTGRIERRTEKSDLGGTMRLGSQRC
                     PVEPGTLAAHIYGADVNERHRHRYEVNNVYVPQLEAKGYKVSARTPSENLPEMMELPG
                     HPFFFGVQFHPEFTSTPRAGHPLFKAFVEAAIAFKRSREPQLARVAA"
BASE COUNT          922 a         2041 c         1979 g         1062 t
ORIGIN      
        1 gttcggcgct ccggcgcccc gcgtgggcga tctggacgac gccaagcgca agatcatcgc
       61 cgacacgacc ccgacctact gcgaatatgc gcgcgagcta gcccgggttc agatcgacgc
      121 tgcgcgcgcg gccgcgtgag cgactcgctg ctgaagttcc tgttccggcg cgcgccggtg
      181 cgcggcggcc tcgtgcgcct ggaccaggcg tggaagcaga tgatcgcgtt ccacgactac
      241 ccggcaccgg tcacgcgcct gctcggcgag atgacggccg cggtagcgct tctggcaacg
      301 aacatcaagt tcaacggtgc gctggtgctg caggtccacg gcgacggccc ggtcaagctg
      361 ctagtcgtcg aatgtcagcc ggacgtcggc ctgcgcgcca ccgccaagct gcgcgccgat
      421 gccgtgatcg acgaagcggc tgggttgcag gcgatggtca acgcgcacgg gcgcggccgc
      481 tgcgcgatca cgctcgaccc gcgcgatccg cagccaggac ggcagccgta tcagggcgtg
      541 gtgccgctgg aaggcgacac gatcgcgcag gcgctcgagt cgtacatgcg gcgctccgag
      601 cagctcgaca cgcacctgcg gcttgccgcc gatggcgaga tcgcggccgg actgctgttg
      661 cagaagctgc cgcgcgaagg cggccgcagc aacgtggccg aggatgcgga cgcgtggggc
      721 cgcgcgctcg cgctcgccgg cacgctgggc ggcgcggaat tgctcgcgca gccgcccgac
      781 acgctcgcgc atcgcctctt ctggcaggag ccactggacg cgtatgcacc gattacaccc
      841 cgtttccaat gcacgtgctc gcgcgaccgt atcgggcgta tgctgatttc gctggggcgc
      901 gaggaagtcg aatcgatcgt cgccgagcag ggccgggtcg aagtgacctg cgatttctgc
      961 aacgcgaagc gcgtgttcga cgcggtcgat gtggcgcagc tgttcgccac cggctcggcg
     1021 gtcgaaatcg acgacaccgc gcgtccgcac tgaggccgga ggccgtatgc aaccccgatg
     1081 gctggcgctg tcagccctgc tcctgctcgc cgcatgcgcg accacgctgc ccgatggcag
     1141 ccggatcgag cgccttcccg cgggcgccac taccagcacg ccgcagctcg gtccgcagga
     1201 ggcgcagaag ctggcgcaac tgaacgcgca agtgctcgcc gaacaggacg ctgcgcgcgc
     1261 ccgtgaggcg cgtgaagagg catggcgccg tgcgcagcag cagctgcaac tcgaccttta
     1321 ctacggaacg ggctggtggg gctggcacga ccgctggggg catcgccatc cacgctggtc
     1381 gttcggcctc ggctggtacg ggccgtggcc gtattgagcc gacccgagct gcggctcgct
     1441 actgcggcgc agccggcttg tcggccttcg gcaccgggac gaactggaag aacagctcgt
     1501 cgttgcggat catgttgagc tgcgatcgcg cacgttcctc gatcgcctcg cgtcccgcct
     1561 tgaagctggc cacctcggcc gcgagctgtg cattgcgcgc cgccagctcg tcattggccg
     1621 cgcgctgcgc cgccagctgg cgatccagtt cagccacccg cagccagccg cccttgccga
     1681 accaaagcgg ccactggatc gccgccagca gcacggcgag cgcgagcgcg agcagcctca
     1741 tgcgcgacta acggaagctg tagaacgcgc tgcgcccagg atagctggcg acgtcgccga
     1801 ggtcctcctc gatgcgcagc agctggttgt acttggcgat gcggtcggag cggctcatcg
     1861 agccggtctt gatctgcagc acgttcaatc cgaccgcaat gtcggcgatc gtcgaatcct
     1921 cggtttcgcc ggagcggtgg ctgacgacgg cggtgtagcc agcgcgcttg gccatctcga
     1981 tcgctgcgaa ggtttccgtc agcgtgccga tctggttgat cttgatcagg atcgaattgg
     2041 cgatgccgcg cgcgatgcct tcgcgcagga tgcgcgtgtt cgtgacgaag agatcgtcgc
     2101 cgaccagctg caccttgcgg ccaagcagct tcgtcagttg cgcccagccg tcccagtcgt
     2161 tctcggccat gccgtcctcg accgacagga tcgggtactt gtcgcaccac gtcgcgagca
     2221 ggttcgcgaa cccgcctgcg tcgagcgtca gcccttcgcc ctcgagcacg tacttgccgt
     2281 ccttgtggaa ctcgttcgct gcgcaatcga gcgccagtgc gacctgcgtg cccgcctcgt
     2341 aaccggcctt gtcgatcgcc tccaggatca gcttgatcgc ctcctcgtgg ctcttcacgc
     2401 tcggcgcgaa gccgccttcg tcgccgaccg ccgtgctcat gtcgcgctcg tggatcagtt
     2461 tcttcagcgc gtggaacacc tcggcgccgt agcgcaccgc ctcggcgaag ctcggcgcgc
     2521 cgaccggcac gatcatgaat tcctgcagat cgagattgtt gttcgcgtgc gcgccaccgt
     2581 tgatcacgtt catcatcggc accggcatct gcatcgcgct gctgcccccg agatagcggt
     2641 acaggggcag gccggactcc tcggccgccg ccttcgccac ggccatcgac acggcaagcg
     2701 tcgcgttggc gccgagccgc atcttgttct cggtgccgtc cagatcgatc agcgtgcggt
     2761 cgaggaacgc ctgttcgttc gcttccaggc ccatcaccgc ctcggcgatc tcggtattga
     2821 tgttctcgat cgccttcagc acgcccttgc cgagatagcg gcccttgtcg ccgtcgcgca
     2881 gttcgatcgc ctcgcgcgag ccggtcgatg cgccggacgg caccgccgcg cgccccatca
     2941 cgccgctttc cagcagcacg tcgcactcga cggtcgggtt gccgcgcgag tcgaggattt
     3001 cgcggccgat caggtcaacg atggaggaca ttgggtttct tctctccggg gttcaaaggg
     3061 tcggcggaac gccttccacg acgatcatgt tgaagtattc cgtcgcgccc ttgcgcttgg
     3121 cgcgcaccgc tcgatactgc tcgccctcgt accacgcctt ggccgcggcg aggctcggga
     3181 acttgaccac cgccacccgt gccggctgcc agtcgccctc gagcgtttcg cagcggccac
     3241 cgcgcgcaag gtattcgcct cccgcggcgg cgatcgtggc cggcgcggcc gccatgtact
     3301 gcttgtactg ctcgggatcg gtgagtttca tctcgaccag cacgtaggca gcaggcattg
     3361 cgcgctcctc agccgaagtt cgtttcgatg aaaccgcgct gcttggtcac gcggtcgagt
     3421 tcgaccagcg ttgcgagcaa ttcggccatg cgatgcagcg gcaccgcgtt gggcccgtcc
     3481 gacagcgcct tggccgggtc cggatgcgtt tccatgaaga ggcctgcgac gccagccgcc
     3541 accgccgcgc gcgccagcac cggcacgaac tcgcgttgcc cgccgctcgc gatgccctgc
     3601 ccgcccggca actgcaccga atgcgtggcg tcgaacacga ccgggcagcc ggtctcgcgc
     3661 atgatcgcga gcgaccgcat gtcggatacg aggttgttgt agccgaagct cgcgccgcgc
     3721 tcgcaggcca gaaaacgatc gtccgacaac ccggcctcgc gcgcagcctc gcgcgccttg
     3781 tctatcacgt tcttcatgtc gtgcggcgcg aggaactggc ccttcttgat gttcaccggc
     3841 ttgccgctga ccgcgcacgc gcggatgaag tcggtctggc gacagaggaa cgcgggcgtc
     3901 tggatcacgt cgacgacctc gaccacgtcg gcgatctcgc cctcggcgtg cacgtcggtc
     3961 agcaccggca cgccgatcct cttcttcacc tcggacagaa tgcgcaggcc ggtttcgatt
     4021 cccagcccgc gaaacgactt tcccgagctg cggttggctt tgtcgtagct cgacttgtag
     4081 atgaacggca tgccgagctt gccggtgatc tccttgagtt gtccggccgt gtcgagcgcc
     4141 atcgtttcgc tctcgatcac gcatgggccg gcgatcagga agatcggccg gtcgagcccg
     4201 gcgtcgaaac cgcagagcct caagccgcca ccctcgccag ctgcggctcg cgcgaccgct
     4261 tgaacgcgat cgcggcctcg acgaacgcct tgaacagcgg atgcccggcg cgcggcgtcg
     4321 acgtgaattc ggggtggaac tgcacgccga agaagaatgg atggcccggc aactccatca
     4381 tttcgggcag gttctcgctc ggcgtgcgcg cggacacctt gtagcccttc gcctcgagct
     4441 gcggcacgta cacgttgttg acctcgtagc gatgccggtg ccgctcgttg acgtcggcac
     4501 cgtagatgtg cgccgcgagc gtgccaggct cgaccgggca ccgttgcgag ccgaggcgca
     4561 tcgtgccgcc gaggtcggac ttctcggtcc ggcgctcgat gcggcccgtg cggtcgagcc
     4621 attcggtgat cagcgccacc accgggtgcg gcgtgtcggg gtcgaactcg gtcgaattcg
     4681 cgcccgccag tccgcacacg ttgcgcgcaa actcgatcgt cgcgagttgc atgccgaggc
     4741 agatgccgag gtaaggcacc ttgtgctcgc gcgcgtactg caccgcccgg atcttgcctt
     4801 cggtgccgcg cctgccgaac ccgccgggta ccaggatggc gtcgagatga tcgagcttga
     4861 ccggcccggg cttctcgaac tgctcggcgt cgacgtactc gatcgtcacc ttggtccgag
     4921 tatggatgcc ggcgtgtatc agcgcctcgt tgagcgactt gtaggaatcc gacagctcga
     4981 cgtacttgcc gaccatgccg atccgcacct cctgctgcgg gtgctccagt gcgtagacca
     5041 gcgcgtccca ggcggagaga tcggccgact tggcgtgcag gccgagcttg tcgcagacga
     5101 tcgcgtcgag gttctgctgg tgcagcatcg ctgggatctt gtagatcgag tcggcgtccc
     5161 acaccgagat cacgctgtcg acgggaacgt tcgaaaacag cgagatcttg gcgcgttcgt
     5221 cctcggggat cggccggtcg gcgcgacaca gcagcacgtc gggatagatg ccgatctcgc
     5281 gcagcttctg cacgctgtgc tgggtcggct tggtcttcag ttcgcccgcg ctcggaatga
     5341 acggcaccag cgtcagatgc acgaagcagc aggaattgcg gcccagccgc aggctcatct
     5401 gccgcgccgc ctcgacgaac ggcagcgatt cgatgtcgcc gaccgtgccg ccgatctcga
     5461 cgatcgcgac atcggtcgcg ccgtcccagg cggcgcgcgg cgcgcgctcg atgaattcct
     5521 ggatttcgtt ggtgatgtgc gggatcacct gcaccgtctt gcctaggtat tcgccgcggc
     5581 gctccttctt gatcaccgac tcgtagatct gtccggtggt gaagttgttc acgcgccgca
     5641 tcttggcgga tatgaagcgc tcgtagtgac cgaggtccag gtcggtctcg gcgccgtcct
     5701 cggtcacgaa cacctcgccg tgctggaacg gcgacatcgt gccggggtcg acgttgatgt
     5761 agggatcgag cttgacgagg gtgactttga ggccgcgcga ttccaggatc gcagcgaggc
     5821 tggcggcagc gatgccctta ccgagcgagg acacgacgcc accggtgacg aagacgtatc
     5881 gggtcattgg tcttccgcct tgtgcgagaa ggcgctaccg gtaaaagcga agtataccga
     5941 cgcgaaacgc gccgcgacgc gcgtggctgc gcgggctcga tcgatcgtca ccgatgccct
     6001 gcga
//
LOCUS       DBNHBY010000434         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_297756, whole genome shotgun sequence.
ACCESSION   DBNHBY010000434 DBNHBY010000000
VERSION     DBNHBY010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_297756"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..975)
                     /locus_tag="AC16HB_10910"
     CDS             complement(202..975)
                     /locus_tag="AC16HB_10910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HIR5607344.1"
                     /translation="MGMTTPFPEPDSPALERFAVCSRVEVLAVLRGLVEQGVLTNVYY
                     DPGGSFFLARLLAVSAESEELVIDPAPDRAVQRRLLESRRLEFVTFVQGVKLQFGAER
                     AEETMFDERPALRVPLPERVIRMQRREFSRVRLPASSPMRCRVRDAGKNETATLRVLD
                     VSSGGLALAFASSEPLFVLGMELDDCQIEIPGEGTITAALRVRSIELAQHAGEKRVGC
                     EFVRIAPKARLALQRFINRADAEQRTGAADRASHLGNDA"
     gene            complement(987..1739)
                     /locus_tag="AC16HB_10915"
     CDS             complement(987..1739)
                     /locus_tag="AC16HB_10915"
                     /inference="COORDINATES: protein motif:HMM:NF018967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HIR5607345.1"
                     /translation="MLKTTLLPESVAVPELAPYVLAARADIVDVLKSLRDGGLTVTCF
                     IEGSFLPAEARIQVVRSDLDTLVMVAVSDLEHDMLAAASAITVVGFQDGVKIQFPAVA
                     NGAVALQGATGVRVSLPTQLLRLQRRAHGRVKPSRVRPLECMVRGAANLPTLQRFPVL
                     DLSVCGVALLGRSRDTYSAGQRLVNCSFNLGQDGEITTDLIVRNVERAEGSGGWRFGC
                     TFAGIGDVALEKVCCHVERIEAHRRASLANEP"
     gene            complement(1733..>1912)
                     /locus_tag="AC16HB_10920"
     CDS             complement(1733..>1912)
                     /locus_tag="AC16HB_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607346.1"
                     /translation="WARRLRGVASIHVLRERWALQMQVDLGPDLPSALYIAVAEVLAW
                     VYVVEGRAAPGESPC"
BASE COUNT          317 a          691 c          615 g          289 t
ORIGIN      
        1 gcgtgctgga gtgcgagtgc ttcgccggtc acctccatct gagcggtgcc gcatgcgtcg
       61 agtgccgccg tcgcttcggc ggttgcatcg ctgcgccgga caccaccgcg atgtcggtcg
      121 gtacgacggt ctggatccgc cccggcggca ccttcgctcg cgtttggcgc gtgagatctc
      181 cccggccgag tacgggccac actacgcgtc gtttcccaag tgcgaagcgc gatccgccgc
      241 ccccgtgcgc tgttcggcat ccgcgcgatt gatgaaacgc tgcaacgcga gccgtgcctt
      301 cggcgcgatg cggacgaact cgcagccgac gcgcttctca cccgcgtgct gtgcaagctc
      361 gatcgagcgc acgcgcagcg cggcggtgat cgtgccttcg cccgggatct cgatctgaca
      421 gtcatccagt tccatgccga gcacaaatag cggctcgctg ctcgcgaacg ccagcgccag
      481 tccgccgctg cttacgtcga gcacgcgcag cgtcgcagtc tcgttcttcc ctgcatcgcg
      541 cacgcggcag cgcatcggcg aactcgccgg caggcgcacg cgagagaatt cacggcgctg
      601 catgcggatc acgcgctcgg gcagcggcac gcgtaacgcc ggtcgttcat cgaacatcgt
      661 ttcctcggcg cgctcggcgc cgaactgcag cttcacgccc tggacgaatg tgacgaactc
      721 caaccgccgc gactcgagca gccggcgctg caccgcgcgg tccggtgcag ggtcgatcac
      781 aagttcctcg gattccgcgc tcactgcaag cagtctggcg aggaagaagc tcccgcccgg
      841 gtcgtagtag acgttggtca gcacgccctg ctcgaccagc ccgcgcagca ccgccagcac
      901 ctcgacccgc gaacacactg cgaagcgctc gagcgcgggc gagtcgggtt cgggaaacgg
      961 cgtagtcatg cccatggtcg tcttcgtcag ggttcgttcg cgagcgacgc gcggcgatgc
     1021 gcctcgatgc gctcgacatg gcagcacact ttctcgagcg cgacgtcacc gatgccggcg
     1081 aaggtgcagc cgaagcgcca gccgcccgag ccttcggcgc gctcgacatt gcgcacgatc
     1141 aggtcggtcg tgatctcgcc gtcctggccg aggttgaagc tgcagttcac cagccgttgc
     1201 ccggccgaat aggtatcgcg cgaacgaccc agcagcgcga cgccgcagac gctgaggtcg
     1261 agcacgggga atcgctgcag cgtcggcagg ttggcagcgc cgcgcaccat gcactcgagc
     1321 ggccggacgc gcgacggctt gacgcggcca tgcgcgcgcc gctgcagccg cagcaactgc
     1381 gtcggcagcg acacccgcac gccagtcgcg ccctgcagcg cgactgcgcc gttggcgacc
     1441 gcgggaaact ggatcttgac cccgtcctgg aagccgacca cggtgattgc gctcgccgcg
     1501 gccagcatgt cgtgctcgag atcgctgacg gcgaccatca ccagcgtgtc cagatcggac
     1561 cgcacgactt gaatgcgcgc ctcggccggc aggaagctac cctcgatgaa gcaggtgacc
     1621 gtcaagccgc cgtcgcgcag gctcttgagc acgtcgacga tgtccgcgcg cgccgccagc
     1681 acatacggcg cgagctcggg cacggcgacg gattccggca gcagcgtcgt cttcagcatg
     1741 gagactcccc aggtgcggcg cgcccttcga ctacatatac ccatgccagc acttcggcca
     1801 ctgcaatgta caacgcagac ggcagatccg gacccaggtc cacctgcatc tgcagggccc
     1861 accgctcgcg caacacgtgg atcgacgcga ccccgcgcaa gcgccgcgcc ca
//
LOCUS       DBNHBY010000435         4375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1312748, whole genome shotgun sequence.
ACCESSION   DBNHBY010000435 DBNHBY010000000
VERSION     DBNHBY010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1312748"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="AC16HB_10925"
     CDS             complement(<1..1376)
                     /locus_tag="AC16HB_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HIR5607347.1"
                     /translation="MLQSIDPRTGAAFGQPLAESTAADIDAAVAAALSAFESFSTSDG
                     ATRAALLRGLAAALEAQREELVQLADRETALGRARLSGELDRTAFQLRGFAQQAEDGA
                     PFLFADDPAVAGAPPAGRPHLTRVRVPIGPVAMFAASNFPFAFSVLGGDTASALAAGC
                     TVVVKAHPGHPQLSHAVFQLAREVIAAQGLDSGVMTLVQGASNDVGIRLVRHPDIAAV
                     AFTGSLRGGAALQAEINARSRPIPFYGELGSINPIVALPGELAARGAELAKTLAASIT
                     LGCGQFCTSPGVVLVFDDAASQTFVDTLKQELAAITPHAMLTPSIKRGFEAGVARFAG
                     HPQVRTLLAATAGGNAPTPMLAETSAAAFIADPSLHDEVFGPACLLVRVKSVDEAIKV
                     LQSVGGSLTATLWGAQVDSADAQRLVRAAQRIAGRVLFAGVPTGVAVTGAQQHGGPWP
                     SSTQPFTTS"
     gene            1716..2714
                     /locus_tag="AC16HB_10930"
     CDS             1716..2714
                     /locus_tag="AC16HB_10930"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR5607348.1"
                     /translation="MFIKTAITRRLGIRHPVLLAPMDLVADARLTTAVSAAGGLGILG
                     GGYGEAQWLVRQLDLLAQSGARFGVGFITWSMAKQPQLLDIALERKPAAVMLSFGDPA
                     RFADRIKRAGALLICQVQSVAIGQQAVAAGADVLVVQGTEGGGHGAARGVISLVPEVI
                     DTVGTAVPVVAAGGIADGRGLAAALMLGASGVLMGTRFYATEEAAGAAAAKERICAAS
                     GDDTVRSVVFDISRRNVWPAPFTGRCLRNAHLERWLGRELELMRHLDEEADKYFAARS
                     AGNFDVAAVIAGESSGLVRAIASAREVIEHTVREAATLLAQGRQHVEISTTSWSAD"
     gene            complement(2721..3632)
                     /locus_tag="AC16HB_10935"
     CDS             complement(2721..3632)
                     /locus_tag="AC16HB_10935"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR5607349.1"
                     /translation="MASLPGRVSIVDVGPRDGLQNEKQMIPAAVKVELVDRLTAAGFA
                     NIEVTSFVSPKWVPQMGDAAEVMAQIRRKPGVVYSVLTPNMKGFEGALAAKADEIVVF
                     GAASEAFSQRNINCSIAESIERFAPVCEAAKKNGIRIRGAISCALGCPYQGEVKPDAV
                     GYVAQLLKQIGVQHCGVADTIGVGTPRATQAAMAAALKHFDLADVSGHFHDTYGQAVG
                     NVYACLEMGISTFDTSVAGLGGCPYAKGATGNVATEDVLYLLRGLGIETGIDLDQVVA
                     TGQWISGILNRKAFARAGNAITAKKAA"
     gene            3738..>4375
                     /locus_tag="AC16HB_10940"
     CDS             3738..>4375
                     /locus_tag="AC16HB_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5607350.1"
                     /translation="MGVQERGQVRARRTTTKASKAAPERRRAMGTRADRGASAVEQLA
                     ATSEILRAISRSPGDTQPVFDTIAASALRLCHATSANVFTYDGTLLRMAAQAVEDPRA
                     VDALRQVFPRPADRGTAASRAVLTRSAVAIHDVIEDPDYVFRSGAQLGFRSVLGLPLL
                     RNGTPIGAIAVGRSEPGPYPERQIALLQTFADQAVIAIENARLFRELQARNR"
BASE COUNT          665 a         1499 c         1539 g          672 t
ORIGIN      
        1 gaggtcgtga acggctgcgt tgacgacggc cacggcccgc cgtgctgctg ggcgccggtg
       61 accgccacgc cggtcggcac gccggcaaac agaacgcggc cggcgatacg ctgcgccgcg
      121 cgcaccagcc gctgcgcgtc ggcactgtcg acctgcgcgc cccagagcgt cgcggtcagg
      181 ctgccgccga ccgactgcag caccttgatc gcctcgtcga ccgacttcac gcgcaccagc
      241 agacacgccg ggccgaagac ttcgtcgtgc agcgaagggt cggcgatgaa tgccgctgcg
      301 ctggtttccg ccagcatcgg cgtcggcgca ttgccgccgg cggtcgcggc gagcagcgtg
      361 cgcacctgcg gatgtcccgc gaacctcgcc acgccggctt cgaagccgcg cttgatcgac
      421 ggcgtcagca tcgcgtgcgg cgtgatcgcg gcaagctcct gcttcagcgt atcgacgaat
      481 gtctgcgatg ccgcgtcgtc gaacaccagc accacaccgg ggctggtgca gaactggccg
      541 caaccgagcg tgatcgaagc ggccagcgtc ttcgccagtt ccgcgccgcg cgcggcgagc
      601 tcgcctggca acgcaacgat cggattgatc gaacccagct cgccgtagaa cgggatcggc
      661 cgcgatcgcg cattgatctc ggcctgcagc gccgcgccgc cgcgcagcga accggtgaac
      721 gccacggccg cgatgtccgg atgccgcacg agccggatgc ccacgtcgtt cgacgcgcct
      781 tgcaccagcg tcatcacccc gctgtcgagt ccctgcgcgg cgatcacctc gcgcgcgagc
      841 tgaaacacgg cgtgcgaaag ctgcggatgc cccggatgcg ccttgaccac gacggtgcat
      901 ccggccgcga gagcggacgc ggtgtcgccg ccgagcacgg agaatgcgaa cgggaaattg
      961 ctcgccgcga acatcgccac cggtccgatc ggcacgcgca cgcgcgtcag gtgcggccgt
     1021 cccgcaggcg gtgcgccggc gaccgcggga tcgtcggcaa agagaaacgg cgcgccgtcc
     1081 tcggcctgct gcgcgaagcc gcgcagctga aacgcggtac ggtcgagttc gccgctcagc
     1141 cgtgcccgtc cgagcgcggt ctcgcgatcg gcaagctgca ccagttcctc gcgctgcgcc
     1201 tcgagcgcgg ccgcgagccc gcgcagcagt gccgcgcgcg tcgcgccgtc cgaggtcgaa
     1261 aagctttcga acgcagacag cgctgcagcg accgcggcgt ctatatcggc ggcggtggat
     1321 tcggcgagcg gctggccgaa agcagcaccg gttctcggat cgatcgattg cagcatggcg
     1381 tgcggtgctc cccggcaacg gtcctggcgc tcgcccgcgc atggcgggaa cgaggcgtag
     1441 cgttatcgta cggccaacga acaggccaac gaaaacgccg tgaagtcatg gatcgccgcc
     1501 gcttcgtccg cgcgcttgcc gccaccgcca ccgctgccgg atgcggtgat gcggcggcgc
     1561 atgcagcagg cgtgggcgca cgcctgatcc acgtcgtgcc gcgcaggcgg gtgcgggacg
     1621 actttcgaaa tggacaccgg cgcagttgcg gacgatgatc gctgcgggaa aggcatgtgc
     1681 gcggtcggcc cgccacgatc ccctcgcgag gtcccatgtt catcaagacg gccattaccc
     1741 ggcggctcgg catacggcat cccgtgctgc tcgcgccgat ggatctggtg gcggatgcgc
     1801 gactgacaac cgcggtgagc gccgccggcg gtcttggcat cctcggcggc ggctacggtg
     1861 aagcgcagtg gctggtccgc caactcgatc tgctcgcgca atcgggcgcg cgtttcggcg
     1921 tcggcttcat cacctggagc atggcgaaac agccgcagtt gctcgacatc gcgctcgagc
     1981 gcaagccggc cgccgtcatg ctgtccttcg gcgatccggc gcggtttgcc gatcggatca
     2041 agcgggcggg cgcgttgctg atctgccagg tgcagtcggt ggcgatcggg cagcaggcgg
     2101 tcgcagccgg cgccgatgtg ctcgtcgtgc agggcacgga aggtggcgga cacggcgcgg
     2161 cgcgtggtgt gatctcgctg gtgccggaag tcatcgatac cgtcggcacc gcagtaccgg
     2221 tggtggctgc gggcgggatc gcggatggcc gcggtctggc cgctgcgctc atgctgggcg
     2281 cctccggcgt gctgatgggt acccgcttct acgcgaccga ggaggcagcc ggcgcagcgg
     2341 cggcgaagga gcgcatctgc gcggcgagcg gggacgacac ggtgcgcagc gtcgtgttcg
     2401 acatctcgcg ccgcaacgtc tggccggcgc ctttcaccgg gcgctgcctg cgcaacgcgc
     2461 acctcgagcg ctggctcggg cgcgaactgg agttgatgcg ccacctcgac gaggaggcgg
     2521 acaagtactt cgctgcgcgc agcgccggca atttcgacgt ggcggccgtg atcgccggcg
     2581 agtcatccgg gctggtgcgg gcaatcgcgt cggcgcgcga ggtgatcgaa cacacggtgc
     2641 gcgaagcggc gaccttgctc gcgcaggggc ggcagcacgt cgagatctcc acgacctcgt
     2701 ggagcgccga ctgaatcgga tcaggcggcc ttcttcgccg tgatcgcgtt gccggcgcgc
     2761 gcaaaagcct tgcggttcag gatgccgctg atccactggc cggttgcgac cacctgatcg
     2821 agatcgatgc cggtctcgat gccgagtccg cgcagcaggt acagcacgtc ctccgtcgcg
     2881 acgttgccgg tcgcgccctt tgcgtacggg cagccgccga gcccggccac actggtatcg
     2941 aaggtgctga ttcccatctc gaggcacgca tagacgtttc ccacggcctg gccgtaggtg
     3001 tcgtggaagt ggcccgacac gtcggcgagg tcgaagtgct tgagcgccgc tgccatcgct
     3061 gcctgcgtgg cgcgcggcgt gcccacgccg atcgtgtctg ccacaccgca gtgctgcacg
     3121 ccgatctgct tcaacagctg cgcgacgtag ccgaccgcgt ccggcttcac ctcaccctgg
     3181 tacgggcagc cgagggcgca cgagattgcg ccgcggatgc ggatgccgtt cttcttcgcc
     3241 gcctcgcaca ccggggcgaa gcgttcgatc gactcggcga tcgagcagtt gatgttcctc
     3301 tgtgagaatg cctcgctcgc ggcgccgaag acgacgatct cgtcggcctt cgccgccagc
     3361 gcaccctcga agcctttcat gttcggcgtc agaaccgaat agacgacgcc gggcttgcgc
     3421 ctgatctgcg ccatcacttc ggcggcgtcg cccatctggg gcacccactt cggcgacacg
     3481 aagctggtga cctcgatgtt cgcgaagccc gccgcggtga gccggtcgac cagctcgact
     3541 ttcacggctg cggggatcat ctgcttctcg ttctgcagcc cgtcgcgcgg gccgacgtcg
     3601 acgatggaaa ccctgccggg cagcgatgcc attgcagtct cctgaggcga gcggtcgatg
     3661 ttactgcgag cttgtctgcg attggcaggc gcgccttccg ggcgtatgtt tgccgcgcca
     3721 ggggcgcgga ggtcagcatg ggggtccagg agcgcgggca ggtgcgtgcg cgccggacga
     3781 cgacgaaggc gagcaaagct gcgccggaac gacggcgcgc gatgggaacg cgggccgatc
     3841 gtggcgccag cgcggtcgag cagctggcgg cgaccagcga gatcctgcgc gccatttcgc
     3901 gctcgcccgg cgacacccag ccggtattcg acacgatcgc agcatcggcg ctgcgacttt
     3961 gccatgcgac atcggccaat gtattcacct acgacggcac gctgctgcgg atggcggcgc
     4021 aagcggtcga agatccccgc gcggtcgatg ccctgcgcca ggtctttccg cgaccggctg
     4081 accgcggcac cgccgccagc cgggccgtgc tgacgcgcag cgcggttgcg atccacgatg
     4141 tgatcgaaga tcccgattac gtcttcagga gcggcgcgca gctcggcttc cgcagcgttc
     4201 ttggacttcc gctgttgcgg aacggcacgc cgatcggagc gatcgcggtc ggccgctccg
     4261 agcccggccc gtatccggag cgacagatcg cgctgctgca gacattcgcc gaccaggcgg
     4321 tgatcgcgat cgagaacgcg cggctcttca gggaactgca ggcgcgcaat cgcga
//
LOCUS       DBNHBY010000436         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1313031, whole genome shotgun sequence.
ACCESSION   DBNHBY010000436 DBNHBY010000000
VERSION     DBNHBY010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1313031"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16HB_10945"
     CDS             complement(<1..235)
                     /locus_tag="AC16HB_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607351.1"
                     /translation="MTACVGTYFRELCGNETPRLRRLRLAVVGGAVGGALALLLYALA
                     LIPFTPTIDGKADAVLAARDVKPSVLLAADGTVI"
     gene            514..687
                     /locus_tag="AC16HB_10950"
     CDS             514..687
                     /locus_tag="AC16HB_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607352.1"
                     /translation="MEHSASAASVVVLLAADLAARPPPWIGATDVTAAAWMDSRCGVE
                     VPRGQAITASAAV"
     gene            complement(657..1772)
                     /gene="ald"
                     /locus_tag="AC16HB_10955"
     CDS             complement(657..1772)
                     /gene="ald"
                     /locus_tag="AC16HB_10955"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5607353.1"
                     /translation="MLIGVPREIKVHEYRVGMTPTSVREAATHGHKVLVEMQAGAGIG
                     ATDDDYRKAGAEVVGSAADVFQRAELIVKVKEPQAAERRMLRDGQVLFTYLHLAPDPE
                     QTKDLIASGAICVAYETVTSATGGLPLLAPMSEVAGRLAVQAGARCLEKSAGGMGTLL
                     GGVPGVAPARVVILCAGVVGTHAAQLAVGSGAQVVVIDKSADALRRIDAMFLGRAMTI
                     FSNQDNVEREVLRADLVISGVLIPGGATPRLITRQMLPKMKHGSVIVDVAIDQGGSVE
                     TSRPTTHADPTYVVDGVVHYTVANMPGAVPRTSTYALNNATLPFALALADKGWRKALA
                     DDPHLKNGLNIAAGKVTYKEVAKALNLNYTAADAVIA"
     gene            complement(1828..>2475)
                     /locus_tag="AC16HB_10960"
     CDS             complement(1828..>2475)
                     /locus_tag="AC16HB_10960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR5607354.1"
                     /translation="FDSASFPFGTSQEIDLGYATVRATRLTYVGELGWELYTPAEFAV
                     GVYEQLLAAGRDLGLVNAGYYAIDSLRLEKGYRAWGRELTPDVNPFEAGLSFAVRLDK
                     EGGFRGCDALVRIKSQGVQRRVVSLVVDAPHTNLWGNELILRDGEPVGFVTSAAFGHT
                     IGKPVALGLISNSAGPLDRHWIDAGRYEIDLAGERYAAQVSLRPPYDPDGMRVKS"
BASE COUNT          363 a          866 c          836 g          410 t
ORIGIN      
        1 cgatcaccgt gccgtccgcc gccagcagca cgctcggctt cacgtcgcgc gccgcgagca
       61 ccgcgtccgc cttgccgtcg atcgtcggcg tgaacggaat cagcgccagc gcgtacagca
      121 gcagcgcaag cgcgccgccg accgcgccgc cgacgaccgc cagccgcagg cgccgcagtc
      181 gcggcgtctc gttgccgcag agttcgcgga agtacgttcc gacgcaagcc gtcacgggcc
      241 gtagcagttt ggcgatcaga gcctcgattc gttcacgcat gcaggtcggc gcgcacctgg
      301 acagtggcac gacgagaaga gtaccgtcgg cgacagcggt gccattaacc cgttagtgcg
      361 tcatccagcc gctcgatggg agcgccgcag gatggcgccg gccccaccga agcatcggcc
      421 gatcgccggg ttctcgccac tttgcgcgct gggtaggatc gcgcgatcgt ctcgtcgtgg
      481 cgcagttcga gccagcgccg cgaaagagtg tgcgtggagc attccgcgag cgcggcgagc
      541 gtcgtcgtgc tgctcgccgc ggatctcgct gcgcggccgc cgccgtggat cggcgcgacc
      601 gacgtcaccg cggcggcgtg gatggattcg cgttgcggcg tcgaggttcc gcgcggtcag
      661 gcgatcaccg cgtccgccgc ggtgtagttc aggttcagcg ccttcgccac ctccttgtac
      721 gtcaccttgc cggcagcgat gttcaggccg ttcttcaggt gcgggtcgtc ggcgagcgcc
      781 ttgcgccagc ccttgtcggc aagcgcgagc gcaaatggca gcgtcgcatt gttcagcgcg
      841 taggtcgagg tgcgcggcac ggccccgggc atgttggcga ccgtgtagtg caccacgccg
      901 tcgaccacgt aggtcggatc ggcatgtgtc gtggggcggc tggtttcgac gctgccgccc
      961 tggtcgatcg ctacgtcgac gatcaccgag ccatgcttca tcttcggcag catctgccgc
     1021 gtgatcagcc tcggcgtggc tccgcccggg atcagcacgc cggagatcac gaggtcggcg
     1081 cgcagcacct cgcgctcgac gttgtcctgg ttggagaaga tcgtcatcgc gcggccgagg
     1141 aacatcgcgt cgatccgccg caacgcgtcg gccgacttgt cgatcacgac gacctgcgcg
     1201 ccgctgccga ctgccaactg cgcggcgtgg gtgccgacca cgccggcgca gagaatcacc
     1261 acgcgcgcgg gcgccacgcc cggcacgcct cccagcagcg tgcccatgcc gccggcggat
     1321 ttctcgaggc agcgcgcgcc cgcctgcacc gcgagccggc ccgcgacttc gctcatcggc
     1381 gcgagcagcg gcagtccgcc ggtggcagac gtcacggttt cgtacgcgac gcagatcgcg
     1441 ccgctcgcga tcaggtcctt cgtctgctcg ggatcgggcg ccagatgcag atacgtgaac
     1501 agcacctgac cgtcgcgcaa catcctgcgc tcggcggcct gcggctcctt gaccttgacg
     1561 atcaattccg cgcgctggaa cacgtctgcg gccgaaccga cgacttctgc gcccgccttg
     1621 cggtagtcgt cgtcggtggc gccgatgccg gcgccggcct gcatttcgac gagcaccttg
     1681 tggccgtgtg tagccgcttc gcgcaccgag gtcggcgtca tgccgacgcg gtattcgtgg
     1741 accttgatct cccggggaac gccgatcagc acttgctcct cctgtggttc agggtttgtg
     1801 acccgctgcg cctcgtgaga gcgcggatca ggacttcacg cgcatgccgt caggatcata
     1861 gggcggccgc agcgacacct gcgcggcgta gcgctcgcct gccagatcga tctcgtatcg
     1921 gcccgcgtcg atccagtgcc tgtcgagcgg accggccgaa ttcgagatca gcccgagcgc
     1981 cacgggtttg ccgatcgtgt ggccgaacgc ggccgaggtc acgaagccga ccggctcacc
     2041 gtcgcgcagg atcagttcgt tgccccacag gttcgtgtgc ggtgcgtcga cgactagcga
     2101 cacgacgcgc cgctgcacgc cttgtgactt gatgcggacc agcgcatcgc agccgcggaa
     2161 gccgccttcc ttgtcgagcc tgaccgcgaa cgacaggccg gcctcgaacg gattgacgtc
     2221 gggtgtgagt tcgcgtcccc acgcgcgata gcccttctcc agtcgcagcg agtcgatcgc
     2281 gtagtagccg gcgttcacga gccccaggtc gcgccccgcc gcgagcaatt gttcgtagac
     2341 gccgactgcg aactcggccg gcgtgtacag ctcccagccc agttcgccga cgtaggtgag
     2401 ccgcgtcgcg cgtacggtcg cgtagccgag gtcgatctcc tgcgaggtgc cgaacgggaa
     2461 cgacgcgctg tcgaa
//
LOCUS       DBNHBY010000437         3632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_500773, whole genome shotgun sequence.
ACCESSION   DBNHBY010000437 DBNHBY010000000
VERSION     DBNHBY010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_500773"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="AC16HB_10965"
     CDS             <1..1400
                     /locus_tag="AC16HB_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5607355.1"
                     /translation="KPCVSGAESIVVDVHNKVARVGPAVLKEGQVITIDGSTGAVYFG
                     EVPTVEVEFSKDMATLLEWADESARLQVRANADTPRDAQRARDWGARGIGLTRTERMF
                     NQTDRLHIVQEMILADSKEARIAALDRLRPFQKGDFKGIFKAMDGLPVTVRLLDPPMH
                     EFLPTVAQLELEIAHLRHFRDAIASLEEMPETLKLLNPRLHHQYEGSLGKLLGGLSEF
                     KESHLDDELIARKTEILKKVKAMAEVNPMLGHRGVRLGVTFPEIYEMQIRAILETAAE
                     CLQEGVAVAPEIMVPQVVTVEELKRIGALVERVHAEVEQSSGIEVPLKFGSMIEVVRA
                     CLRAKRMAQTAEFFSFGTNDLTQATFSFSREDAENKFLPLYYEAGLLRDNPFEVLDVK
                     GVGELMKLAIANGRTQRPDLKVGICGEHGGDPTSIRFCHHAGLDYVSCSGPRVPIARL
                     AAAHASLLESEYSDE"
     gene            1404..2255
                     /locus_tag="AC16HB_10970"
     CDS             1404..2255
                     /locus_tag="AC16HB_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like domain-containing protein"
                     /protein_id="HIR5607356.1"
                     /translation="MSVAGEIVSQVERLAGRLSMPVVKALHLPPPNADGTREGEFCAI
                     ELEDGSLGLSFVLLGVPVGATLTALREGGHRSVLPGTPALELARAYGESAPARRTLGF
                     AAINAITRHLYSRAEFKPPPASGSIGDLDLAPGDHLGMIGLFPPLIERVLATGARLTV
                     LELRADVAGARDGWEVTLDPAALAGCNKILSTSTVLLNDTIDDILARCRHAERIALIG
                     PGASCLPDALFARGVTSMGGTWIDDPAGLKQALVSGEPWGRCATKFSLRRERYPGVEG
                     LLARARA"
     gene            complement(2289..3473)
                     /locus_tag="AC16HB_10975"
     CDS             complement(2289..3473)
                     /locus_tag="AC16HB_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5607357.1"
                     /translation="MSEAFIVSALRTAGARKGGRLAGWHPADLAARVLDALVAKLDLD
                     PLLIDDVILGCVGQAGEQSNNIARNAVLASRLPESVPGTSVDRQCGSSQQALHFAAQA
                     VMSGAQDIVIAGGVESMTRVPMGLPATLPYKNGFGSYMSPAMQKRYPGVQFSQFMGAE
                     MLAKKYDLPKEALDEYALASHQRAASATREGRFTAEIVPIPARNAEGAETGETHAADE
                     GIRFDATREGIAGVKLLQEGGRITAASASQICDGAAALLVANERGLKAIGGRAQPLAV
                     VRQMTVVGHDPVIMLEGPVPATPVALRKAGLKLDDIDLFEVNEAFASVPLAWLKATGA
                     DPMKLNVNGGAIALGHPLGASGAKLMTTLVHALRARGKRYGLQTMCEGGGLANVTIVE
                     RV"
BASE COUNT          560 a         1236 c         1246 g          590 t
ORIGIN      
        1 gcaagccgtg cgtgtcgggc gcggaaagca tcgtcgtcga cgtgcataac aaggttgcgc
       61 gcgtcggccc ggcggtgctc aaggaaggtc aggtgatcac gatcgacggc tcgaccggcg
      121 cggtgtactt cggcgaagtg cccaccgtcg aggtcgaatt ctcgaaggac atggccacgc
      181 tgctcgaatg ggccgacgag tccgcccgcc tgcaggtgcg tgccaacgcc gacaccccgc
      241 gcgatgcgca gcgcgcgcgc gactggggcg cgcgcggcat cggcctcacc cgcaccgagc
      301 gcatgttcaa ccagacggat cgtctgcaca tcgtgcagga gatgatcctc gccgattcga
      361 aggaagcacg catcgccgcg ctcgaccggc tgcgcccgtt ccagaagggc gacttcaagg
      421 gcatcttcaa ggcgatggac ggcctgccgg tcaccgtgcg cctgctcgat ccgccgatgc
      481 acgagttcct gccgacggtg gcgcagctcg aactggagat cgcgcatctg cgccatttcc
      541 gcgatgcgat cgccagtctg gaggaaatgc ccgagacgct gaagctgctc aatccccgcc
      601 tgcaccacca gtacgaaggc agccttggca agctgctcgg tgggctctcc gagttcaagg
      661 aatcgcatct cgacgacgag ctgatcgcca ggaagaccga gatcctgaag aaggtcaagg
      721 caatggccga agtcaatccg atgctcggcc accgcggcgt gcggctaggc gtcacgttcc
      781 ccgagatcta cgagatgcag atccgcgcga tcctggagac ggcggccgag tgcctgcaag
      841 aaggtgtcgc ggtcgcgccc gagatcatgg tgccgcaggt ggtgacggtc gaggagctga
      901 agcgcatcgg cgcgctggtc gagcgcgtcc atgccgaggt cgagcaatcg tccggcatcg
      961 aggtgccgct gaagttcggc agcatgatcg aggtcgtgcg cgcctgcctg cgtgcgaagc
     1021 ggatggcgca gacggcggag ttcttctcgt tcggcaccaa cgacctgacg caggcgacgt
     1081 tctcgttctc gcgcgaggac gccgagaaca agttcctgcc gctgtactac gaggcggggc
     1141 tgctgcgcga caacccgttc gaggtgctcg acgtcaaggg ggtcggcgaa ctgatgaagc
     1201 tcgcgatcgc caacggccgc acgcagcgtc cggatttgaa ggtcggcatc tgcggcgagc
     1261 acggcggcga tccgacctcg atccgcttct gccaccatgc cggtctcgac tacgtttcgt
     1321 gctccggccc gcgcgtgccg atcgcgcggc tggctgccgc gcacgcgagc ctgctcgaat
     1381 cagagtacag cgacgagtga cgcatgagcg tcgcgggcga gatcgtttct caggtcgagc
     1441 gtctggcagg gcgactgtcg atgcccgtgg tcaaggcgct gcacctgccg ccgcccaacg
     1501 ccgacggcac gcgcgagggc gaattctgcg cgatcgagct ggaagacggt tcgctgggct
     1561 tgagcttcgt gctgctcggc gtcccggtcg gggcgacgtt gacggcgctg cgcgaaggcg
     1621 gacatcgctc ggtgctgccg ggaacgccgg cactggagct ggcgcgcgcc tacggcgaga
     1681 gcgcgccggc gcggcggacg ctcggtttcg cggcgatcaa cgcgatcacg cgccacctgt
     1741 actcccgcgc ggagttcaaa ccgccgccgg cgagcgggtc gatcggcgac ctggatctcg
     1801 cgccgggcga tcacctcggc atgatcggat tgttcccgcc gctgatcgag cgcgtgctcg
     1861 cgaccggcgc gcggctcacc gtgctcgagt tgcgcgccga cgttgccggc gcgcgggacg
     1921 gctgggaggt caccctcgac ccggcggcgc tcgccggctg caacaagatc ctctccacca
     1981 gcacggtgct gctgaacgac acgatcgacg acatcctcgc gcgctgccgg catgccgagc
     2041 gcatcgcgct gatcgggccc ggtgccagct gcctgcccga tgcgctgttc gcgcgcggcg
     2101 tcacctcgat gggcggaacg tggatcgacg accccgcggg gttgaagcag gcgctcgtga
     2161 gcggcgagcc gtggggtcgc tgcgcgacca agttttcgct acggcgggaa cgctatcccg
     2221 gcgtcgaagg gttgctcgcg cgcgcccgcg cctgagcgag cttgccttgc agtagatcgc
     2281 ccgcgagact agacacgctc gacgatcgtc acattcgcca gcccgccgcc ctcgcacatc
     2341 gtctgcagtc cgtagcgctt gccacgcgcg cgcagcgcat ggaccagcgt ggtcatcagc
     2401 ttcgcgccgg acgcgccgag cggatggccg agcgcgatcg cgccgccgtt gacattcagc
     2461 ttcatcgggt cggcgccggt ggccttcagc cacgccagcg gcaccgacgc gaaggcctcg
     2521 ttgacctcga acagatcgat gtcgtcgagc ttgagccccg ccttgcgcag cgccaccggc
     2581 gtggcgggca ccggtccttc gagcatgatc acggggtcgt ggccgaccac ggtcatctgg
     2641 cgcacgaccg cgagtggctg cgccctgcct ccgattgcct tcaggccgcg ctcgttggca
     2701 acgagaagcg cggccgcgcc gtcacaaatc tggctcgcgc tggcggccgt gatgcgtcca
     2761 ccttcctgca gcaacttgac gccggcgatt ccttcgcgcg tggcgtcgaa gcggatgcct
     2821 tcgtccgccg cgtgcgtttc gcccgtttca gcgccctcgg cgttgcgtgc cgggatcggc
     2881 acgatctccg ccgtgaatcg tccttcgcgt gtcgcgctgg cggcgcgctg gtggctcgcg
     2941 agcgcgtatt cgtcgagcgc ttccttcggc aggtcgtatt tcttcgccag catctcggcg
     3001 cccatgaact ggctgaactg cacgccggga tagcgcttct gcatggcggg gctcatgtag
     3061 ctgccgaagc cgttcttgta cggcagcgtc gcgggcagac ccatcggcac gcgcgtcatg
     3121 ctctccacgc cgccggcgat cacgatgtcc tgcgctccgg acatcaccgc ctgcgcggcg
     3181 aagtgcagcg cctgctgcga cgacccgcac tggcggtcga cggaggttcc gggcaccgat
     3241 tcgggcaggc gcgaggcgag caccgcgttg cgcgcgatgt tgttcgactg ctctccggcc
     3301 tgcccgacgc agccgaggat cacgtcgtcg atcagcagtg ggtcgagatc gagcttcgcg
     3361 accagcgcgt cgagcacccg cgccgcgaga tcggccggat gccagcccgc gaggcgaccg
     3421 cccttgcggg cgcctgccgt gcgcagtgca gagacgatga aggcttcgga catgacggtt
     3481 acctcccgat gcaatggtgt tcgatcctca tgacttctcg cgcaatcgac gtgccgccgc
     3541 ggaggcgagg gtgatcagcg cgtcccgttc gatgccgcgc gcggacaccc acagcgccac
     3601 accgtcgtcc tcggcgagca actgcagttc gc
//
LOCUS       DBNHBY010000438         4631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_771941, whole genome shotgun sequence.
ACCESSION   DBNHBY010000438 DBNHBY010000000
VERSION     DBNHBY010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_771941"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16HB_10980"
     CDS             complement(<1..227)
                     /locus_tag="AC16HB_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5607358.1"
                     /translation="MKRRSITRALLVSAAAATLFGAAGGAFAQQKKEVTIAHQDMLDP
                     YRILMESGEIEKVTGYKVNWRMFGGGGDVIRA"
     gene            complement(348..1532)
                     /locus_tag="AC16HB_10985"
     CDS             complement(348..1532)
                     /locus_tag="AC16HB_10985"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR5607359.1"
                     /translation="MIDHLLTLNAGSSSIKFALFRAELTAAAPRIHGIADGIGATARL
                     RIAGNDGWRDEPLAGASGAPSHADALGAVLRALHAGATDARIVGVGHRIVHGGAQFDR
                     PVVIDEAALQAMRAFEPLAPLHQPHNVAGVRAAMTAFPGVPQVACFDTAFHRTQPELN
                     QRFALPQRYFDAGVRRYGFHGLSYESIVAQLGARARGRVIVAHLGNGASLCAMHDGRS
                     VATTMGFSTLDGMPMGTRCGRLDAAVVLHLLQREAMSADRIAHLLYRESGLAGLSGLS
                     GDMRELEAADTPMARTAIDYFVEHALRELAAMAAALRGLDRIVFTGGIGENAADLRRR
                     IVEGGGWLGWRLDADANRAGRERISAPDSSAEVLVLHTNEETVIARHTARCIAGTRGH
                     GA"
     gene            complement(1534..2328)
                     /locus_tag="AC16HB_10990"
     CDS             complement(1534..2328)
                     /locus_tag="AC16HB_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5607360.1"
                     /translation="MPKVVKLAPRARKPAVHASSSTLKAFAVLETLVRTGHPTTLAEL
                     MLATGLPKPSLHRTLALFEEASLVVREPTGRAYAVGPRLGRFALDVLTHDYVATQRRV
                     ILRKLVADVGETCNLSMLRKGEVFYLDRVEADWPLRLHLPVGSQLPPHCCASGKLLLA
                     FKEPAERAQLVAALALPRFTDRTITDRHMLASELDRIAANGYAVDNEEYVVGVACVAV
                     PVRLPAGDVVAAVAVHAATARLPLARAIEFVSTLNGAALAIAKTFS"
     gene            2510..4297
                     /gene="xsc"
                     /locus_tag="AC16HB_10995"
     CDS             2510..4297
                     /gene="xsc"
                     /locus_tag="AC16HB_10995"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR5607361.1"
                     /translation="MTDMKQPNRSVVQGRTKMTPSEAFVESLVAQGVTDVFGIVGSAY
                     MDALDILPAAGIRFIPVAHEQGAVHMADGYARVSGRHGVCIGQNGPGITNFVTGVAAA
                     FWAHSPVVVITPETGSMTMGLGGFQETEQLPIFSRITKYQGHVNNPKRMAEIASRAFD
                     RALLEMGPTQLNIPRDFFYGDIECEIPQPIRIGRGAGAESALDEAAKLLAEAKFPVIL
                     SGGGVIMGDGMAEAIKLAELLHAPVANSYLHNDSFPASHPLACGPLGYQGSKAAMKLI
                     AQADVVLALGSRLGPFGTLPQYGLDYWPKNAKIIQIDADAKMLGLVKPISIGICGDAK
                     AAASALVRRLQGRSLACLQNQAERDARIKAEKAVWEKELDGWTQETDPYSIEVSRKSS
                     HMHPRQMLRELEKAMPANAMVSTDIGNICSVSNSYLRFEVPRSMFAAMSFGNCGYAFP
                     VICGAKVAAPNRPAIAYVGDGAWGISLNELLTCARERIGVTVVVFNNGQWGAEKKNHV
                     DFYSQRYVGVELENPSWAGVAKSFGCDGMTIDKLSDVGPALAQAVKNQAEGRTTVLEM
                     MVTKELGDPFRRDALSKPVRHLDKYRNYV"
     gene            complement(4299..>4631)
                     /locus_tag="AC16HB_11000"
     CDS             complement(4299..>4631)
                     /locus_tag="AC16HB_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5607362.1"
                     /translation="CNGLAAIGGLAAVVMLLSTTVAVASTRATLIGLFLFTDVYSLAV
                     AATHDLVGRAMLVATAWLVPPMLAGVAFGSRWYARTDADRFKRYVIFLLMGLALLGIA
                     SSALHFAR"
BASE COUNT          776 a         1550 c         1601 g          704 t
ORIGIN      
        1 gcgcggatca cgtcgccgcc gccgccgaac atccgccagt tgaccttgta gcccgtgacc
       61 ttctcgatct cgccggactc catcaggatc cgatacggat ccagcatgtc ctggtgcgcg
      121 atcgtgacct ccttcttctg ctgcgcgaac gcgccgccgg cagcgccgaa cagcgttgcc
      181 gcggcggccg aaaccagcaa tgcacgggtg atgctgcgtc tcttcatgaa ctcctccttc
      241 ggttgacttg ttgcgccggc tacctgttca cggccgacgc aggctacgct cgctggactc
      301 accgtcaata gtgccagcag gcggtattgc cccgggccat gcagtgctca tgcgccatgg
      361 ccgcgtgtgc cggcgatgca gcgcgcggtg tggcgcgcga tcaccgtttc ctcgttcgtg
      421 tgcagcacca gcacctcggc gctgctgtcg ggcgcggaga tgcgttcgcg gccagcgcgg
      481 ttggcatccg cgtcgagacg ccagccgagc cagccgccgc cctcgacaat gcgccggcgc
      541 aggtcggctg cgttctcgcc gatgccgccc gtgaacacga tgcggtcgag tccgcgcagc
      601 gccgcggcca tggcggccag ttcgcgcagc gcgtgctcga cgaaatagtc gatcgcagtg
      661 cgcgccatgg gggtgtccgc ggcctccagt tcacgcatat cgccggacag gccggacaac
      721 ccggccagcc ccgactcgcg atacagcagg tgcgcgatcc gatcggcgct catggcctcg
      781 cgctgcagca ggtgcagcac gacagccgca tcgagtcggc cgcagcgcgt gcccatcggc
      841 attccatcga gcgtcgagaa gcccatcgtg gtcgcgaccg agcgcccgtc gtgcatcgcg
      901 cacagcgacg cgccattgcc cagatgcgcg acgatgacgc gtccgcgcgc gcgcgcgccg
      961 agctgcgcga cgatcgactc gtacgacagg ccgtggaagc catagcggcg cacgccggca
     1021 tcgaagtagc gctgcggcag cgcgaagcgc tggttgagct cgggctgcgt gcggtggaac
     1081 gcggtgtcga agcacgcgac ctgcggcacg ccgggaaacg cggtcatcgc cgcccgcacg
     1141 ccggcgacat tgtgcggctg gtgcagcggc gccagcggct cgaacgcgcg catagcctgc
     1201 agtgccgctt catcgatcac gaccggacgg tcgaactgcg cgccgccgtg cacgatgcgg
     1261 tggccgacgc cgacgatgcg cgcgtcggtg gcacctgcat gcagcgcgcg cagcacggcg
     1321 cccagcgcgt cggcatgcga cggtgcgccg ctcgcacccg cgagcggctc gtcgcgccag
     1381 ccatcgttgc cggcgatgcg cagccgcgcg gtcgcgccga tcccgtcggc gatgccgtgg
     1441 atgcgcggcg ccgccgcggt cagttcggcg cggaacagcg cgaacttgat cgacgacgag
     1501 ccggcgttca gcgtcagcag atgatcgatc atgtcaggag aacgtcttcg cgatcgccag
     1561 tgccgcgccg ttcagcgtcg atacgaactc gattgcgcgc gccagcggca ggcgcgcggt
     1621 ggccgcgtgc accgcgaccg cagcgaccac gtcgccagcg ggcagccgca cgggcacggc
     1681 gacgcaggcc acgccgacca cgtactcttc gttgtcgacc gcatagccgt tggcggcaat
     1741 gcgatcgagt tcgctcgcga gcatgtgccg gtccgtgatc gtacggtcgg tgaagcgcgg
     1801 cagcgccagc gcggcgacga gttgcgcgcg ttccgcgggc tccttgaacg ccagcagcag
     1861 cttgccgctg gcacagcaat gtggcggcag ctggcttccg acgggcagat gcagccgcag
     1921 gggccagtcc gcctcgacgc gatccaggta gaagacttcg cccttgcgca gcatcgacag
     1981 gttgcaggtc tcgccgacat cggcaaccag cttgcgcagg atcacgcgcc gctgcgtggc
     2041 gacgtaatcg tgggtcaaca cgtcgagcgc aaaacggcca agccgcggcc cgaccgcgta
     2101 cgcgcgcccg gtcggctcgc gcaccacgag gctcgcttcc tcgaacagcg ccagcgtccg
     2161 gtgcagcgac ggcttgggca gccccgtcgc gagcatcagt tcggcgagcg tcgtggggtg
     2221 gcccgtgcgc accagggtct cgagcaccgc aaacgccttc agcgtcgacg agcttgcgtg
     2281 caccgccggc ttgcgcgcac gcggcgcgag cttgacgact ttcggcatgc ggggatggta
     2341 tcggccgggt tccatgtccg caatcggcac gttcaggtga caggaacagg tgcttgcgtt
     2401 tcgtttcccg atcgcctagc gtttctttca agcgaacaaa tcatttcatc caccggtacg
     2461 gtggcctagg ccgtcgtgcc ggccgttttc attcgatgcg gagcagccga tgaccgacat
     2521 gaagcagccc aatcgcagcg ttgtccaggg ccggacgaag atgacgccct ccgaggcgtt
     2581 cgtcgaatcg ctggttgcgc agggcgtgac cgacgtgttc gggatcgtcg ggtcggccta
     2641 tatggatgcg ctcgacatcc ttccggcggc gggaatccgc ttcatcccgg tggcgcacga
     2701 gcagggcgcg gtgcacatgg cggacgggta cgcgcgcgtt tcgggccggc acggcgtgtg
     2761 catcggccag aacggtcccg gcatcacgaa cttcgtcacc ggcgtggccg ctgcgttctg
     2821 ggcgcattcg ccggtggtcg tgatcacgcc ggaaaccggc tcgatgacga tggggctggg
     2881 cggcttccag gagaccgagc agttgccgat cttctcgaga atcaccaagt accagggcca
     2941 cgtgaacaac ccgaagcgga tggccgagat cgcaagccgc gccttcgacc gcgcgctgct
     3001 ggaaatgggg ccgacgcaac tgaacatccc gcgcgacttc ttctacggcg acatcgagtg
     3061 cgagatcccg cagccgatcc gcattggccg cggcgccggc gcggagtccg cgctcgacga
     3121 ggcggcgaag ctgctggcgg aggcgaagtt ccccgtgatt ctgtccgggg gcggcgtgat
     3181 catgggcgac ggcatggccg aggcaatcaa actggccgaa ctgctgcatg cgccggtcgc
     3241 caacagctac ctgcacaacg actcgttccc ggcgtcgcat ccgctcgcgt gcggaccgct
     3301 gggctatcag ggcagcaagg cggcgatgaa actgatcgcg caggccgacg tggtgctcgc
     3361 gctcggctcg cggctcgggc ccttcggtac gctgccgcag tacgggctgg actactggcc
     3421 gaagaacgcg aagatcatcc agatcgacgc cgacgcgaag atgctcgggc tcgtcaagcc
     3481 gatctcgatc ggcatctgcg gcgacgccaa ggcggccgcg tcggcgctcg tacggcggct
     3541 gcaaggccgg tcgctcgcgt gcctgcagaa ccaggccgag cgcgacgcca ggatcaaggc
     3601 cgagaaggcg gtatgggaga aggagctcga cggctggacc caggaaaccg atccgtactc
     3661 gatcgaggta tcgaggaagt cgagccacat gcatccgcgc cagatgctgc gcgagctgga
     3721 aaaggcgatg ccggccaacg cgatggtgtc gaccgacatc ggcaatatct gctcggtgtc
     3781 caattcgtac ctgcgcttcg aggtgccgcg ctcgatgttc gctgcgatga gcttcggcaa
     3841 ctgcggctat gcgtttccgg tgatctgcgg cgccaaggtc gcggcgccga accgtccggc
     3901 gattgcctac gttggcgacg gtgcgtgggg catcagcctg aacgaactgc tgacctgcgc
     3961 gcgcgagcgc atcggcgtga ccgtggtggt gttcaacaac ggacaatggg gcgcggagaa
     4021 gaagaaccac gtcgacttct actcgcagcg ctacgtcggc gtcgagctgg agaatccgag
     4081 ctgggccggt gttgcgaagt cgttcggctg cgacggcatg accatcgaca agctgtccga
     4141 cgtcgggccg gcgctcgcgc aggcggtgaa gaaccaggcc gaaggccgca ccaccgtgct
     4201 cgagatgatg gtcaccaagg agctcggcga cccgttccgc cgcgacgcgc tgtcgaagcc
     4261 ggtgcggcac ctcgacaagt acagaaacta cgtgtgattc agcgggcgaa atgcagcgcg
     4321 ctgctcgcga tgccgagcag cgcgagcccc atcagcagga agatcacgta gcgcttgaag
     4381 cggtcggcat cggtgcgcgc gtaccagcgg ctgccgaacg cgacgcccgc cagcatcggc
     4441 ggcacgagcc aggcggtggc caccagcatt gcacggccga cgaggtcgtg cgtcgctgcc
     4501 accgcgagcg aatagacgtc ggtgaagagg aagaggccga tcagcgtggc gcgcgtcgat
     4561 gcgaccgcaa ccgtggtcga cagcaacatc accacggcgg cgagcccgcc gatcgcggcc
     4621 agcccgttgc a
//
LOCUS       DBNHBY010000439         3224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_568192, whole genome shotgun sequence.
ACCESSION   DBNHBY010000439 DBNHBY010000000
VERSION     DBNHBY010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_568192"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1005
                     /locus_tag="AC16HB_11005"
     CDS             1..1005
                     /locus_tag="AC16HB_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607363.1"
                     /translation="MMIRFSRSLLAVAALSAALALPTAFAQSTAYPSKPIHLIVPYPP
                     GGPLDTTARALAERVRDSLGVVVVENRPGAGGNIGVDYVAKQPADGYTLAIGAVATHA
                     INPWLFAKLPYDPLKDFAPITLIAHVPNVLVMAPGTAAKYQISSFADLVAYARAHPGQ
                     LNYASGGNGSGGHMAGELLKSMGRVFIVHIPYAGAAPAQLGLLSGQTDLMFDNLASAA
                     AQIRAGKLKAFAVTTKSRAAGLPEVPTVAEAGGKDFAGFDISTWFGVFAPAGTPAPIV
                     ERLSDEFRKALASGDMRERLARMGAEPAPMTPSDFAAFVRSELAKYEKVVKFSGAKVD
                     "
     gene            1100..1561
                     /locus_tag="AC16HB_11010"
     CDS             1100..1561
                     /locus_tag="AC16HB_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5607364.1"
                     /translation="MRIRNEKDFWSGVMFIAFGLFFALFAQKYDFGTAQRMGPAYFPT
                     VLGGILVLLGVVIAITGLGREDADGKIEKFHFAEAGWVLGSVVVYGLLLRPAGALLSI
                     VALVAISSMGSHEFRWKEVILLAIAMAVVTWAVFIWGLGVTIPVWPAFMER"
     gene            1574..3085
                     /locus_tag="AC16HB_11015"
     CDS             1574..3085
                     /locus_tag="AC16HB_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5607365.1"
                     /translation="MELLSNLALGFGTALSFTNLFYALMGCLIGTLIGVLPGIGPVAT
                     LAMLLPTTYSLDPTSALIMLAGIYYGAQYGGSTTSILVNIPGESSSVVTCIDGYQMAR
                     RGRAGAALGIAAIGSFIAGCFSTVLVAAFAPPLTELAFKFGPAEYFPLMVLGLIGAVV
                     LASGSLVKAIAMIILGLLLGIVGTDVNSGVARFSFDVPELTDGLGFVGVAMGVFGFAE
                     IIANLEKKEHREVFTSKIGTLLPNLKEFVYSLPAIGRGTVLGSLLGILPGGGALLASF
                     ASYTMEKKVSEKSAEIPFGKGNIRGVAAPESANNAGAQTSFIPMLTLGIPPNAVMALM
                     IGAMTIHNIQPGPQVMTSNPSLFWGLIASMWIGNLMLVVLNLPLVGVWVQLLKVPYRL
                     LYPAILVFCAIGVYAVNNTTFDVMLTAVFGALGYVFYKLKCEPAPLILGFILGPMMEE
                     NLRRAMLLSRGDPMTFVTRPLSLGLLLAALALLLIVTLPAIKSKREEAFQEAD"
BASE COUNT          503 a         1033 c         1078 g          610 t
ORIGIN      
        1 atgatgatcc gcttcagccg ttccctgctc gccgtggccg cgctgtctgc cgcgctggcg
       61 ctgccgaccg cattcgcaca gtcgacggcc tacccgagca agccgatcca cctgatcgtt
      121 ccgtatccgc cgggcggccc gctcgacacc acggcgcgcg cgctggccga gcgcgtgcgc
      181 gactccctcg gcgtggtcgt ggtcgagaac cgccctggcg ccggcggcaa catcggcgtc
      241 gattacgtcg ccaagcagcc ggccgacggc tacacgctcg caatcggtgc agtggccacg
      301 catgcgatca acccgtggct gttcgcgaag ttgccgtacg acccgctcaa ggacttcgcg
      361 ccgatcacgc tgatcgcaca tgtgccgaac gtgctggtga tggcgcccgg gacggcggcg
      421 aagtaccaga tcagttcgtt cgccgacctc gtcgcgtatg cgcgcgcgca tcccggacag
      481 ctgaactacg cctccggtgg caacggcagc ggcggccaca tggcgggcga gctgctgaag
      541 tcaatgggcc gcgtgttcat cgtgcacatc ccctacgccg gtgccgcgcc ggcgcagctc
      601 gggctgctgt ccgggcagac cgacctgatg ttcgacaacc tcgcctcggc agcggcgcag
      661 attcgcgccg gaaaactgaa ggcgttcgcg gtcacgacga aatcgcgcgc cgccggcctg
      721 cccgaggtgc cgacagtcgc cgaggccggc ggcaaggatt tcgccggctt cgacatcagc
      781 acgtggttcg gcgtgttcgc gccggccggc acgccggcgc cgatcgtcga gcgcctgagc
      841 gacgagttcc gcaaggcgct cgcgagcggc gacatgcgtg aacggctggc gcgcatgggg
      901 gcggagccgg cgccgatgac accttccgac ttcgccgcgt tcgtgcgcag cgagctcgcc
      961 aagtacgaga aggtcgtgaa gttctcgggc gcgaaggtcg actgaagatt cacgcggcaa
     1021 cgcgagcgat cggctctaga atcgccgggc cagaacacgg acgcggtgcg caataaaccg
     1081 cgcgatcaag gaggggcctt tgaggatccg gaacgagaag gacttctggt ccggcgtgat
     1141 gttcatcgcg ttcgggctgt tcttcgcgct gtttgcgcag aagtacgact tcggcaccgc
     1201 gcagcggatg gggccggcgt atttcccgac ggtgctcggc ggcattctcg ttctgctagg
     1261 cgtcgtgatc gctatcacgg gactcggacg ggaagacgcc gacggcaaga tcgagaaatt
     1321 ccacttcgcc gaggcgggct gggtgctcgg ctcggtcgtg gtgtacggcc tgctgctgag
     1381 gcctgcgggc gcgctgcttt cgatcgtggc gctggtggcg atctcgagca tgggcagcca
     1441 tgagtttcgc tggaaggaag tcatcctact tgccatcgcg atggccgtcg tcacttgggc
     1501 cgtcttcatc tggggcctcg gcgtgacgat tccggtgtgg ccggccttca tggaacgctg
     1561 agcggagaag cgcatggagc tgctttcgaa tctcgccctc gggttcggca cggcgctgtc
     1621 gtttacaaac ctcttctatg cgctgatggg atgcctgatc ggtaccctga tcggcgtgct
     1681 gcccggcatc ggcccggtgg cgacgctcgc gatgctgctg ccgaccacgt actcgctcga
     1741 cccaacctcg gcgctgatca tgctcgccgg catctactac ggtgcgcagt acggtggctc
     1801 gaccacctcg atactggtca acatccccgg cgagtcctcg tcggtggtga cttgcatcga
     1861 cggctaccag atggcgcgac gcgggcgtgc cggtgctgcc ttgggcatcg cggcgatcgg
     1921 ctcgttcatc gccggctgct tctcgaccgt tctggtggca gcctttgcgc cgccgctcac
     1981 tgaactggca ttcaaattcg gccccgccga gtacttcccg ctgatggtgc tcggcctgat
     2041 cggggccgtc gtgctggcgt caggatccct ggtcaaagcg atcgcgatga tcatcctcgg
     2101 gttgctgctc ggcatcgtcg gcactgacgt gaactcgggc gtggcgcgct tctccttcga
     2161 tgttccggag ctcacggacg gcctcggttt cgtcggcgtg gcgatgggcg tgttcggctt
     2221 cgccgaaatc atcgccaatt tggagaagaa ggaacatcgc gaggtcttca ccagcaagat
     2281 cggcacgctg ctgccgaatc tcaaggaatt cgtctattcg ctgccggcga tcggacgcgg
     2341 caccgtgctc ggatcgctgc tgggaatcct gcctggcggc ggcgcgctgc tcgcgtcgtt
     2401 cgcgtcgtac acgatggaaa agaaagtcag cgaaaagagc gccgagattc ccttcggcaa
     2461 gggcaacatc cgcggcgtgg cggctcccga atcagcgaac aacgcgggtg cgcagacctc
     2521 gttcattccg atgctgacgc tcggcattcc acccaacgcg gtgatggcgc tgatgatcgg
     2581 cgcgatgacc atccacaaca tccagccggg cccgcaggtg atgacgagca atccgtcgtt
     2641 gttctggggc ctgatcgcgt cgatgtggat cggcaacttg atgctggtgg tcctgaacct
     2701 gcctctggtc ggtgtgtggg tgcagttgtt gaaggtgccg taccggctgc tttatccggc
     2761 gatcctcgtc ttctgcgcga tcggggtgta tgcggtgaac aacaccacgt tcgatgtaat
     2821 gctcaccgcc gtgttcggcg cgctcggcta cgtgttctac aagctcaagt gcgagccggc
     2881 accgttgatc ctcggcttca tcctcgggcc gatgatggaa gagaacctgc ggcgggcaat
     2941 gttgctgtcg cgcggcgatc ccatgacgtt cgtcacgcgc ccgctgtcgc tcggcctgct
     3001 gctggccgcg ctggcgctgc tgctgatcgt cacgctgccg gcgatcaagt ccaagcgcga
     3061 agaggcgttc caggaagcgg attaggctgc cgcggttcta gttgatcgcc gtcgccgacg
     3121 gcgcgaggac gaccgtgcga tcgagggaga ccacgtcgcc cgatgcttga ctacccgcaa
     3181 gcatgccggc gacggtcgcg ccgagcaccg ccgcgctcat gagc
//
LOCUS       DBNHBY010000440         6729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1785810, whole genome shotgun sequence.
ACCESSION   DBNHBY010000440 DBNHBY010000000
VERSION     DBNHBY010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1785810"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1087
                     /locus_tag="AC16HB_11020"
     CDS             101..1087
                     /locus_tag="AC16HB_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5607366.1"
                     /translation="MAPVIDVHTHMLNHDYLALLEMHGAPRLTMKEVVGGLRAIHLDD
                     APFMTPVPQMFDYGARIRMMDEVGIDVSIVSLTCPNVYFGSSEVSLQAARVMNDDMAA
                     ARARHAGRIEYFCSLPWQYAEPAVAELARARSKGARGVMVLANIAGRPLTAPEFAPVW
                     QAIDAAALPVLVHPTAPPGVREMQMNEFQLTASIGFTFDTTLATSRLLFDGFFDRYTN
                     LKIIASHGGGALPFLLGRLDQCFRNIPACRTRTQSAPSSYMGRLYADSVVFTQEALDL
                     ALHAFGDDYVMYGSDYPHTIGDAPGCLRRVNSLFGAQREKVRGKNAQRIFGI"
     gene            1104..2054
                     /locus_tag="AC16HB_11025"
     CDS             1104..2054
                     /locus_tag="AC16HB_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607367.1"
                     /translation="MKRRTFTLAALATPLASLAQDKPPIRILVGFPPGGSADVIARAL
                     ADKLKNTLGQSLIVDNKPGAAGRVALGELRRAAPDGQTLILSPSGALVIAPYLFKNVG
                     YDPAKDFAPIARVVTFDFAVTAGRAAPPGDLNAVLSWMKANPAKANYATPGAGTVPHF
                     AGLLLAQTAGVAITHIAYKGGAPAVQDLLGGQVPLMIDTPLETIEHHRAGKLRILAVT
                     GEARSRALPDVPTLKEAGVDMVADAFFGLYGPAGLPSAVSTRLSNAVRDILRAPDLQE
                     RIQVLGLVPAYGTSEELAATQAAHYQRWDAPVKASGITPE"
     gene            complement(2061..4130)
                     /locus_tag="AC16HB_11030"
     CDS             complement(2061..4130)
                     /locus_tag="AC16HB_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HIR5607368.1"
                     /translation="MTMSRLGTDGSVAKAEKLLKYWDGRASAIGLGMVPDSSAGPRRF
                     NDRDAARLAALATRVPVTTGARLGEIFLEWAVRHAQIRLGSYFNSAKVLFFSGLAQQK
                     LAQSMAEYTDNLQFADPLLQLGVPKLLTSLEALSLYARGAHYASNWTPPRVMTAGLLK
                     DWSGYVLRKAMQSATVIVAPVHELDEFGLEELGGKTIITSTVNDERIAQFRDKGVNMI
                     VDGSPFMFHHVLGPNLLDAMIIAATGKNSDDLLEDDYLEIITDLQIEPRIIYPNGFRR
                     VNRFAFVIHPLSQEYFKKVKSIDLLSRVSPPVFMDALERVMAYAPPFVYSRVTGIKSP
                     TGVEAEGWLISVGGTPKEIMSHSPEFTYRRLLDAAKMAKRLGAQIMGLGAFTKVVGDA
                     GVTVAKRAPMPITTGNSYSASGALWAAHDAVLRMGLLPKPVGRERVKFKAMVVGATGA
                     IGSVCSRLLVKAAEEVYLVSPETAKLLALKESILQETPDAKMFLSARADKDVAAMDMI
                     VTATSGAGKKVLDIMKVKPGCVITDVARPLDLPPEEVAKRPDVLVIESGEIHLPGDVK
                     MKNIGLPKGVAYACLAETIVLALEGRYENFTVGRAIEWEKVREIYKMGLKHGMKLAAI
                     SGVNGPFSDEDIERVRELALAARAKQAAAERRAEQRVAKLARTRPVARPRKAAPRRKA
                     AEARAAA"
     gene            4317..5630
                     /locus_tag="AC16HB_11035"
     CDS             4317..5630
                     /locus_tag="AC16HB_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR5607369.1"
                     /translation="MTQNTLADLVRTIGLPTAKRAAGVVTRLIPIPRPTLLVGPGASA
                     RLGHAVADFGHRKILLVTDEIIVGLGLLTSLTDALSAGGTRFVVYDEITADAPIPLIE
                     KGIKLFKREDCDAIVAFGGGSVMDAAKVIGLAAANRKHPRQLVGYFRGLRAPAPIYAV
                     PTTAGTGSEVTVAAVISDPERHRKLVIADTRIVPRMAALDPTLMVGLPPHITAATGMD
                     ALTHAIEAFIGYWTTDTSDRMALSAVSMIYENLPRAFDNGSDLAAREKMALASTYAGF
                     AFTRANVGNVHAIAHQLGGRYHTPHGLANAIMLPHVLRFESAAITGKLATLALRAKVG
                     QPGEPETERAARFLDSIDALNDRLKIPRHLDALRAEDIPALAKAACWEADTNYPVPRT
                     MSQEDCEGLLRRLLPPPHAAKRATPAASRRTSARPRRTPRRAVDA"
     gene            complement(5635..6030)
                     /locus_tag="AC16HB_11040"
     CDS             complement(5635..6030)
                     /locus_tag="AC16HB_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5607370.1"
                     /translation="MITIPSAWTQRAGVLLLRWAVAGLLLLHGVAKLQRGLGGVESLL
                     SSKGLPAWFAFGVLIGEVIAPLLVMVGVLVRPAALVMALNMVVAVALAHPAQVFELGR
                     TGGYALELQALFFFGALAIALLGSGRAKT"
     gene            complement(6101..>6729)
                     /locus_tag="AC16HB_11045"
     CDS             complement(6101..>6729)
                     /locus_tag="AC16HB_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607371.1"
                     /translation="QTWRRRNCVADNGQLFLTGYSEGGYVTVAAHRALQATGSPHLSS
                     LAAVVPGAGPYHVTVTLDALLDRVQNEVPVLGALITPKLLRFLPVSVRNQLRDEMLKR
                     LVPADADVLFDTTILDNYLADDDAAIERQSNVHDWAPIAPIRLFHGRDDQTVTYAVAT
                     QTLQTMLARGARDISLTDCPAVPSSHLGCVPPFIAFMVGQLAARARNL"
BASE COUNT         1124 a         2349 c         2144 g         1112 t
ORIGIN      
        1 ccgagttcgc tgcttacgtg aagagcgaac aggcgcgctg gcacaggatc gtcgtcgatt
       61 ccggcgccag ggcggactga tgcacaaagg agaaaccggc atggcccccg tcatcgacgt
      121 ccacacccac atgctgaacc acgactacct cgccctgctg gagatgcatg gcgcacccag
      181 gctgacgatg aaggaagtgg ttggtgggct gcgcgcgatc cacctcgacg acgcgccatt
      241 catgacgccg gtaccgcaga tgttcgacta cggcgcgcgc atccggatga tggacgaagt
      301 cggcatcgac gtttcgatcg tgtcgctgac ctgccccaat gtctacttcg gatcttccga
      361 ggtcagcctg caggccgcgc gcgtcatgaa cgacgatatg gccgccgcgc gcgcgcgcca
      421 cgcgggccgc atcgagtact tctgctcgct gccgtggcag tacgccgagc ccgcggttgc
      481 ggaactcgcg cgcgccagga gcaagggcgc acgcggcgtg atggtgctcg cgaacatcgc
      541 cggccggccg ttgacggcgc ccgaattcgc gccggtatgg caggcgatcg acgcggccgc
      601 gctgccagtc ctcgtacatc ccaccgcgcc gcccggggtg cgcgagatgc agatgaacga
      661 attccagctc accgcgtcga tcggtttcac cttcgacacg acgctcgcaa cttcgcgcct
      721 gctgttcgac ggtttcttcg accgctacac gaacttgaag atcatcgcgt cgcacggcgg
      781 cggcgcgctg ccgttcctgc tcggccggct cgaccagtgc ttccgcaata ttcccgcctg
      841 ccgcacccgg acccagagtg cgccgtcgag ctacatgggg cgcctgtatg ccgactccgt
      901 cgtgttcact caggaagcgc tcgacctcgc gctgcacgcg ttcggcgacg actacgtgat
      961 gtacggctcc gactacccac acacgatcgg cgacgcaccc ggctgcctgc ggcgggtgaa
     1021 ttcgctattc ggcgcgcaac gcgagaaggt gcgcgggaag aacgcgcagc gtatctttgg
     1081 aatctgagca gagaggagag cccatgaaac gccgcacctt cacgctcgcc gcgctcgcga
     1141 caccgctggc atcgctggcg caggacaaac ctccgatccg catcctggtc gggttcccgc
     1201 ccggcggttc tgccgatgtc atcgcgcgcg cgctcgccga caagttgaag aacacactcg
     1261 gccagtcctt gatcgtcgac aacaagccgg gcgcagcggg ccgtgtcgcg ctcggcgaat
     1321 tgcgccgcgc cgcacccgac ggccagacgc tgatcctctc gccgagcgga gcgctggtga
     1381 tcgcgccgta tctgttcaag aacgtgggct acgacccggc caaggacttc gcgcccatcg
     1441 cgcgcgtggt gaccttcgac ttcgcggtca ccgccggccg cgcggccccg ccgggcgacc
     1501 tgaatgcggt gctctcctgg atgaaggcca acccggccaa ggccaactac gccacgccgg
     1561 gcgcgggcac cgtaccgcac ttcgctggat tgctgttggc gcagacggcg ggcgtcgcga
     1621 tcacccacat cgcatacaag ggcggcgcgc cggcggtgca ggatctgctc ggcggtcagg
     1681 tgcccttgat gatcgacacc ccgctggaga cgatcgaaca tcatcgcgcc ggcaagctgc
     1741 gcatcctggc ggtgactggc gaggcgcgtt cgcgcgcact gcccgacgtg ccgacgctga
     1801 aggaggccgg cgtcgatatg gtcgccgatg cgttcttcgg tctatacggt ccggcaggat
     1861 tgccttccgc cgtgtcgacg cgcctgtcga acgcggtgcg cgacatcctg cgtgcgccgg
     1921 atctgcagga gcgcattcaa gtgctcgggc tggtgccggc gtacggaacc tccgaggaac
     1981 tcgcggccac gcaggcggcg cattaccagc gctgggacgc gccggtcaag gcgtctggca
     2041 tcacgcccga gtgatccgtc tcaggcagca gcgcgcgctt ccgctgcttt cctgcgcggt
     2101 gccgccttgc gcggccgtgc aaccggccgc gtacgcgcca gctttgcgac ccgctgctct
     2161 gcgcgccgtt ccgcggccgc ctgcttcgcg cgcgcggcaa gcgcgagttc gcgcacgcgc
     2221 tcgatgtcct cgtccgagaa cggtccgttg acgcccgaga tcgcggcgag cttcatgccg
     2281 tgcttcagcc ccattttgta gatctcgcgc accttttccc actcgatcgc gcggccgacg
     2341 gtgaagttct cgtaccgccc ttcgagcgcc agcacgatcg tctcggccag acaggcgtag
     2401 gccacgccct tcggcaggcc gatgttcttc atcttcacgt cgccgggcag atggatctcg
     2461 ccggactcga tcaccagcac gtcgggccgt ttcgccacct cttccggcgg caggtcgagc
     2521 gggcgtgcga cgtcggtgat cacgcagccg ggcttgacct tcatgatgtc gagcaccttc
     2581 ttgcccgcac ccgaagtggc ggtcacgatc atgtccatgg cggcgacgtc cttgtccgcg
     2641 cgggccgaca ggaacatctt cgcgtccggc gtctcctgca ggatcgactc cttcagcgcc
     2701 agcagcttcg cggtctcggg cgagacgagg taaacctcct ccgcggcctt gaccagcaga
     2761 cgcgagcaca ccgagccgat cgcgccggtc gcccccacca ccatcgcctt gaacttcacc
     2821 cgctccctgc cgaccggctt cggcagcagg cccatgcgca gcaccgcgtc gtgcgcggcc
     2881 cacagcgcgc cggatgcgct gtagctgttg ccggtcgtga tcggcatcgg cgcgcgcttg
     2941 gccaccgtga cacccgcgtc gccgacgacc ttggtgaacg cgcccagtcc catgatctgc
     3001 gcgccgagcc gcttcgccat cttcgccgcg tccaggagcc gccgatacgt gaactcgggg
     3061 ctgtggctca tgatttcctt cggcgtgccg ccgaccgaaa tcagccagcc ctccgcctcc
     3121 acgccggtcg gcgacttgat gcccgtgacc cgcgaataga cgaagggcgg cgcgtacgcc
     3181 atcacgcgct ccagcgcgtc catgaacacc ggcggcgaca cgcgcgacag caggtcgatc
     3241 gacttgacct tcttgaagta ctcctgcgac agcggatgga tcacgaacgc gaagcggttc
     3301 acgcgccgga agccgttggg atagatgatg cgcggctcga tctgcaggtc cgtgatgatt
     3361 tccagatagt cgtcttcgag caggtcgtcc gaattcttgc cggtcgcggc gatgatcatc
     3421 gcatcgagca ggttcgggcc cagcacgtgg tggaacatga acggcgagcc gtcgacgatc
     3481 atgttcacgc ccttgtcgcg gaactgcgcg atgcgctcgt cgttgaccgt cgacgtgatg
     3541 atcgtcttgc cgccgagttc ctcgaggccg aactcatcca gctcgtgcac cggcgccacg
     3601 atcacggtcg cgctctgcat cgccttgcgc agaacgtagc ccgaccagtc cttcagcaat
     3661 cccgccgtca tcacccgcgg cggcgtccag tttgacgcgt aatgcgcgcc gcgcgcatac
     3721 agcgacaagg cctcgagcga tgtcagcagc ttcggcacgc cgagttgcag cagcgggtcg
     3781 gcgaactgca ggttgtcggt gtactccgcc atcgactgcg cgagtttctg ctgcgccaga
     3841 ccagagaaga acagcacctt ggcgctgttg aagtagctgc cgagcctgat ctgtgcgtgc
     3901 cgcaccgccc actcgaggaa gatctcgccc agcctcgcgc cggtcgtcac cggcacgcgc
     3961 gtcgccagtg ccgccagccg tgccgcatcg cggtcgttga agcgccgtgg cccggcgctg
     4021 ctgtccggga ccatgcccag cccgatcgca ctcgcgcgcc cgtcccagta cttgagcagc
     4081 ttctccgcct tggcaacgct gccgtccgtg ccgagacgac tcatcgtcat cctctgtccg
     4141 aggaagcggg cggcgaatcg aaaatcctgg ctgctggacc caaggctgat gctgacgatg
     4201 tgtttcatct gatgtgcgct ggcgacgatc gccgcttcaa ttgacgattt cgtaggcggg
     4261 cccggtgcaa acctgccacg ctcggcgtca gtcgaccaca ctgagcggag gacatcttga
     4321 cccagaacac gcttgccgac ctcgtgcgca cgatcggcct gccgacggcc aagcgcgcgg
     4381 ccggtgtcgt cacccggctg atcccgattc cgcgcccgac cctcttggtc ggcccgggcg
     4441 cgagcgcgcg gctgggccac gcggtcgcgg actttggtca ccggaagatc ctcctcgtca
     4501 ccgacgagat catcgtcggt ctgggcctgc tgacttcact gaccgacgcg cttagcgccg
     4561 gcggcacgcg cttcgtcgtg tacgacgaga tcacggccga cgcgccgatt cccctgatcg
     4621 agaaaggcat caaactcttc aagcgcgagg actgcgacgc gatcgtcgcc ttcgggggtg
     4681 gctcagtgat ggacgcggcc aaggtcatcg gcctggcggc tgccaaccgc aagcatccgc
     4741 gccaactggt cggctacttt cgcgggctgc gcgctccggc gccgatctat gcggtaccga
     4801 ccaccgccgg caccggttcc gaagtgacgg tcgccgcggt gatctccgac ccggagcgtc
     4861 accgcaagct cgtgatcgcc gacacgcgaa tcgtgccgcg catggcggcg ctcgatccca
     4921 ccctgatggt cgggctgccg ccgcacatca ccgccgctac cggcatggat gccctcacgc
     4981 acgcgatcga ggcttttatc ggctactgga cgaccgatac gtccgatcgc atggcgctgt
     5041 ccgccgtcag catgatctac gagaacctgc cgcgtgcgtt cgacaacggc agtgacctgg
     5101 ccgcgcgcga gaagatggca ctggcctcga cctatgccgg cttcgcgttc acgcgcgcca
     5161 acgtgggcaa cgtgcatgcc atcgcgcacc aacttggcgg ccgataccac acaccacacg
     5221 ggctcgccaa cgcgatcatg ctgccgcacg tgctgcgctt cgagagcgcc gccatcaccg
     5281 gcaagctggc cacgctcgct ctgcgcgcga aggtcggcca gccgggcgaa cccgagaccg
     5341 agcgggcagc caggttcctc gactcgatcg acgcgctcaa cgaccgactg aagatcccgc
     5401 gtcacctcga tgcactgcgc gcggaggaca tccctgcctt ggccaaggcc gcttgttggg
     5461 aggccgacac caactatccg gtgccgcgca cgatgtcgca agaggattgc gagggtttgc
     5521 tgcggcggct gctgccgccg ccgcatgcgg cgaagagagc cactccggcg gcgagtcgac
     5581 gaacctctgc acgaccgcgg cgtacgccgc gacgcgcggt cgacgcctga agcttcaggt
     5641 cttcgctcgg ccgctgccga gcagtgcgat cgccagagcg ccgaagaaga agagcgcctg
     5701 caactcgagc gcgtagccgc cggtcctgcc gagttcgaat acctgcgccg ggtgcgcgag
     5761 cgccacggcc accaccatgt tcaacgccat cacgagcgcg gctggtcgaa cgagcacgcc
     5821 gaccatcacc agcaacggcg caattacctc gccgatcagc acgccgaatg cgaaccaggc
     5881 cggcagccct ttgctcgaca gcagcgactc cacgccgccg agcccgcgct gcagcttcgc
     5941 gactccgtgc aggagcagca gaccagcgac tgcccagcgc agcaacaaca ccccggcacg
     6001 ctgggtccat gcggacggaa tcgtgatcaa gcggaggctc ctgaggtatg catacgggcc
     6061 gtgtcctgaa cggtacgaga tgtagtgaca ccgcgcgcgg ctacaggttt cgcgcgcggg
     6121 cggcgagttg accgaccatg aaggcgatga atggagggac acagccaagg tggctcgatg
     6181 gcaccgcagg gcagtcggtc agggaaatat cgcgggcgcc gcgcgcgagc atggtctgca
     6241 gcgtctgcgt cgcgacggca taggtcacgg tctgatcgtc gcggccgtgg aagagccgga
     6301 tcggtgcgat cggcgcccag tcgtgcacgt tgctttggcg ttcgatcgcc gcgtcgtcat
     6361 cggcgaggta gttatcgagg atcgtcgtgt cgaacaatac gtccgcgtcc gccggcacca
     6421 ggcgcttgag catttcatcg cgcaactggt tgcggacgga aacgggcagg aaccgcaaca
     6481 gcttcggcgt aatcagcgcg ccgagcacgg gaacctcgtt ttgtacgcga tcgagcaatg
     6541 catccagtgt cacggtgacg tgatacggac cggccccggg gacaaccgct gccagacttg
     6601 atagatgggg actgccggtc gcctgcaacg ctcgatgcgc agcgacggtc acatacccgc
     6661 cctcggaata gcccgtcaga aacagttgtc cgttgtcggc gacacagttc cgccgccgcc
     6721 atgtctgcg
//
LOCUS       DBNHBY010000441         1767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1583198, whole genome shotgun sequence.
ACCESSION   DBNHBY010000441 DBNHBY010000000
VERSION     DBNHBY010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1583198"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..606
                     /locus_tag="AC16HB_11050"
     CDS             178..606
                     /locus_tag="AC16HB_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607372.1"
                     /translation="MNGNRVDGPHSDMNLIDSSRSVLTNREVKQMNKALLAATLTVAV
                     LAACGKQAPAPAPAPAPAPAAAPAPAPAPAQQAVEAAKDAAGQAVDATKDAAAKAADA
                     TKEAAGKAVDATKEAAGKAVDAAKEAAGKAVDAAKEAAKK"
     gene            complement(676..1449)
                     /locus_tag="AC16HB_11055"
     CDS             complement(676..1449)
                     /locus_tag="AC16HB_11055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5607373.1"
                     /translation="MRFCSLGSGSEGNALVVEVHDGLTVTRLLVDNGFGPRTLAKRLG
                     RASMAIDDLDAVVVTHEHSDHVAGVAALARKRRIAVFASAGTARAARIESDEIEVRRL
                     HAGEPVAIGGVHVTPYAVAHDAAEPLQFVFSDGAVRLGLLTDIGAPDADVARALDGLD
                     ALILECNHDEQMLAGGDYPPFLKARIAGDRGHLSNAQAAALLATIDRSRLTYVAAAHL
                     SRSNNRSLLARRALAAVLGCRDDEVGVAHQDDGLEWRTL"
     gene            complement(1465..>1767)
                     /gene="bamC"
                     /locus_tag="AC16HB_11060"
     CDS             complement(1465..>1767)
                     /gene="bamC"
                     /locus_tag="AC16HB_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR5607374.1"
                     /translation="WRRVGLALDRGGFTVEDRDRTKGTYFVRYLDPEFEAKQRENQGF
                     FSRMFGRDPKIDPQQFRVQVAAAGDATSVTVLDKEGKPDGGATAQKILGQINEQLK"
BASE COUNT          295 a          628 c          570 g          274 t
ORIGIN      
        1 gaggcctggc tgcatcaccg cggctacgcg caaccgctgc tcgcgacgtt cggtcggcgc
       61 tgggatcgcg ccgcgcacaa cctggccgtg acagcgctcg ggctgtagtc gccttcgctg
      121 cggcgcggca tcaagagccg cctcgcgtat aatggctgat tgggtagatc atcctggatg
      181 aacggcaacc gtgtcgacgg gccgcattca gacatgaacc tgatcgactc gtcgcgatcc
      241 gtacttacca accgtgaggt caaacaaatg aacaaagcac ttcttgctgc aacgctgacg
      301 gtggcggttc tcgccgcctg tggcaaacaa gctccggcac ccgccccggc gcccgcgcct
      361 gcccccgctg ccgcgcctgc tccggcgccc gcgcctgctc agcaggctgt cgaagccgcg
      421 aaggacgctg ccggccaggc cgtcgacgcc accaaggacg cggccgccaa ggctgcggac
      481 gcaaccaagg aagccgctgg caaagccgtc gatgcgacca aggaagccgc cggcaaagcc
      541 gtcgatgctg ccaaggaagc cgccggcaaa gccgtcgatg ctgccaagga agctgcgaag
      601 aagtagtcgc accgccccgc agcgggcgct acgtcaaggg ccggctcgcc ggccctttgc
      661 ttttctgagc ctcgcttaaa gcgtgcgcca ctcgagtccg tcgtcctggt gcgcgacgcc
      721 gacttcgtca tcgcggcagc caagaacggc ggccagcgca cggcgtgcca gcagcgagcg
      781 gttgttgctg cgactcagat gcgcggcggc aacatatgtg agacgtgagc ggtcgatcgt
      841 cgccagcagt gccgccgcct gcgcattcga cagatgtccg cgatcgccgg caatgcgcgc
      901 cttcaggaac ggcggatagt cgccgcccgc gagcatctgc tcgtcgtgat tgcattccag
      961 gatcaacgcg tcgagtccgt cgagcgcacg cgccacgtcc gcatcgggcg caccgatgtc
     1021 ggtcaggagt ccgagccgaa ccgcgccgtc ggaaaacacg aactgcagcg gttcggccgc
     1081 gtcgtgcgcc accgcgtacg gcgtcacgtg gacaccgccg atcgcgaccg gttcgccggc
     1141 gtgcaaccgg cgcacttcga tttcgtcgga ttcgatgcgc gccgcgcgcg cggtgccggc
     1201 cgatgcgaag acagcgatgc gccgcttgcg cgccagcgcg gccacgccgg cgacgtgatc
     1261 cgaatgttcg tgcgtgacga cgaccgcatc cagatcgtcg attgccatgg acgcgcgccc
     1321 cagccgcttt gccagcgtgc gcggcccgaa tccgttgtcg accagcaggc gcgtgacggt
     1381 cagcccgtca tgcacctcga ccaccagcgc attgccctcg ctgcccgagc cgagactgca
     1441 gaaccgcagc acggcggcgc gccgctactt cagttgctcg ttgatctggc ccaggatctt
     1501 ctgcgccgtc gcgccaccat cgggcttgcc ttccttgtcg agcacggtca cggacgtcgc
     1561 gtcgcccgct gcggccacct gtacgcggaa ctgctgcgga tcgatcttgg ggtcgcgccc
     1621 gaacatgcgt gagaagaagc cctggttttc gcgctgcttg gcctcgaact ccgggtccag
     1681 gtaacgcacg aagtacgtgc ccttcgtgcg gtcgcgatcc tcgacggtga acccgccgcg
     1741 atcgagcgcc agcccgacgc gccgcca
//
LOCUS       DBNHBY010000442         2287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1246190, whole genome shotgun sequence.
ACCESSION   DBNHBY010000442 DBNHBY010000000
VERSION     DBNHBY010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1246190"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="AC16HB_11065"
     CDS             <1..172
                     /locus_tag="AC16HB_11065"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547279.1"
                     /note="catalyzes the formation of acetyl phosphate from
                     acetyl-CoA and phosphate; can also act with other
                     short-chain acyl-CoAs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR5607375.1"
                     /translation="ADSAGIVLGTRVPIVLTSRSDSVRTRLASTAILALVAHARRSKL
                     GETAPSLTPPAR"
     gene            complement(206..1006)
                     /locus_tag="AC16HB_11070"
     CDS             complement(206..1006)
                     /locus_tag="AC16HB_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR5607376.1"
                     /translation="MTPDVLRIGDRTFHSRLLLGTGKYKDLAQTKAAVDASGAEIVTV
                     AIRRTNIGQNRNEPNLLDVLPPAHYTMLPNSAGCYTAEDAVRTLRLARELLDGHALTK
                     LEVLGDPATLYPNMPETIKAAETLVKEGFEVMVYCSDDPIQAQVLESIGCVAVMPLAS
                     LIGSGMGILNPWNIQLILEKAKVPVIVDAGVGTASDAAIAMELGCAGVLMNTAVAQAK
                     DPVLMASAMRKAVEAGREAFRAGRMPKKLYSATPSSPTAGVIAPSKAA"
     gene            complement(1018..1479)
                     /locus_tag="AC16HB_11075"
     CDS             complement(1018..1479)
                     /locus_tag="AC16HB_11075"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5607377.1"
                     /translation="MSQSHLFEGALRWTGKADDVDGKLRLERALVIEFAGKAPITASS
                     PAVFNGDDAQHNPESLMIASLMSCHLLTYLAVCERAGIRVLSYEDRATGTLAIKDGKM
                     RMVEVVLRPRVQIADGAQIERALASHGKAHANCFMSNSVNFEVKVEPVMSA"
     gene            complement(1479..1679)
                     /gene="thiS"
                     /locus_tag="AC16HB_11080"
     CDS             complement(1479..1679)
                     /gene="thiS"
                     /locus_tag="AC16HB_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR5607378.1"
                     /translation="MAIVVNGETRRVAAGATVADLLAQMGAAGKRVAVERNGAIVPRS
                     AHRETKLAAGDRLEVVIAVGGG"
     gene            complement(1719..>2287)
                     /locus_tag="AC16HB_11085"
     CDS             complement(1719..>2287)
                     /locus_tag="AC16HB_11085"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5607379.1"
                     /translation="HGLDLAALISDHGYWVLALGTLLEGETVLALAGFAAHQGYLSLP
                     LVVAIAATAGFCGDLAWFWLGRRHGEVVLMRLPSVAAQAERMQRLIERHHEWVIVGIR
                     FAYGLRIAGPVLIGTSNVPAWRFALFNALGAILWAPLIAGLGWVFGAAVERALGNLRQ
                     IELWLFATLAAIGAALWLLQRHRAGRRR"
BASE COUNT          354 a          811 c          758 g          364 t
ORIGIN      
        1 cgcggacagc gccggcatcg tgctcggtac gcgcgtgccg atcgtcctga cgagccgctc
       61 cgacagcgtg cgcacgcggc tggcgtccac cgccatcctg gcgctcgtgg cgcacgcccg
      121 gcgcagcaag ctgggcgaaa cggcgccttc gctcacgccg cccgcaagat gattgatcgc
      181 ggtggcgccg ctccggcgcc gatgatcagg ccgccttgct cggagcgatg acgccagccg
      241 tcggcgaact cggcgtcgcg ctgtagagct tcttcggcat gcggccggcg cggaacgcct
      301 cgcgtcccgc ctcgaccgcc ttgcgcattg cgctcgccat cagcaccggg tccttcgcct
      361 gcgccacggc ggtgttcatc agcacgcccg cgcagccaag ctccatcgcg atcgccgcat
      421 cgctggccgt gccgacgccg gcgtcgacga tcaccggcac cttcgccttc tcgaggatca
      481 gctggatgtt ccagggattg aggatcccca tgccggagcc gatcagcgag gcgagcggca
      541 tcaccgccac gcagccgatc gactcgagca cctgcgcctg gatcggatcg tcgctgcagt
      601 agaccatcac ctcgaagcct tccttcacca gggtctcggc cgccttgatg gtctccggca
      661 tgttggggta gagcgtggcc ggatcgccga gcacctcgag cttggtgagc gcgtgcccgt
      721 cgagcagttc gcgcgcgagc cgcagcgtgc gcaccgcgtc ctcggccgtg tagcagccgg
      781 cgctgttggg cagcatcgtg tagtgcgcgg gcggcagcac gtcgagcagg ttcggctcgt
      841 tcctgttctg gccgatgttg gtgcggcgga tcgcgaccgt cacgatttcg gcgccgctgg
      901 catcgaccgc ggccttcgtc tgcgccaggt ccttgtactt gccggtgccg agcagcagcc
      961 gcgagtggaa ggtgcgatcg ccgatcctca ggacgtcggg ggtcatctca tcgcgtttca
     1021 tgcggacatc actggttcga ccttcacttc gaaattgacc gaattggaca tgaagcagtt
     1081 ggcgtgcgcc ttgccgtgcg atgccagcgc gcgctcgatc tgtgccccgt cggcgatctg
     1141 cacgcgcggg cgcagcacga cctcgaccat ccgcatcttg ccgtccttga tcgccagcgt
     1201 gccggtcgca cggtcctcgt acgacaggac gcgaatgccg gcgcgctcgc ataccgccag
     1261 ataggtgagc agatggcacg acatcagcga cgcgatcatc agcgattccg ggttgtgctg
     1321 ggcatcgtcg ccgttgaaga ccgccggcga cgaggcggtg attggcgcct tgccggcgaa
     1381 ctcgatcacc agcgctcgct cgagcctcag cttgccgtcg acatcgtcgg cctttccggt
     1441 ccagcgcagc gcgccttcga acaggtgcga ctggctcatc agccgcctcc gaccgcgatc
     1501 acgacctcga gccggtcgcc ggcggccagc ttcgtctctc ggtgcgcgct gcgtggcacg
     1561 atcgcgccgt tgcgttcgac tgcaacgcgc ttgccggcgg cgcccatctg cgcgagcagg
     1621 tcggcgacgg tggcgccggc ggccacgcgc cgcgtctcgc cgttgacgac gatggcgatc
     1681 gcttcgatca cgttctccat ggcgagcatg ttagtccatc agcgccggcg cccggcgcga
     1741 tgacgttgca gcagccacag cgcggcgccg atcgcagcca gcgtggcaaa gagccacagt
     1801 tcgatctgcc gaaggttgcc cagcgcgcgc tcgaccgctg cgccgaacac ccagccgagt
     1861 ccggcgatca gtggcgccca aaggatcgcg ccgagcgcat tgaaaagcgc gaaacgccac
     1921 gcgggtacgt tcgaggtgcc gatcaggacg ggcccggcga tccgcaagcc gtaggcgaaa
     1981 cgtatgccga cgatcaccca ctcgtgatgg cgctcgatca ggcgctgcat ccgctccgcc
     2041 tgcgccgcca ccgacggcag gcgcatcagc acgacttcgc cgtgccggcg gccgagccag
     2101 aaccaggcca ggtcgccgca gaagcccgcc gtcgcggcga tcgcgaccac gaggggaaga
     2161 gaaagatagc cctggtgcgc agcgaagccc gccagcgcga gcacggtctc gccctcgagc
     2221 agcgtgccga gggcgagcac ccagtagcca tggtccgata tcagcgcggc cagatccaag
     2281 ccatgct
//
LOCUS       DBNHBY010000443         3649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_433199, whole genome shotgun sequence.
ACCESSION   DBNHBY010000443 DBNHBY010000000
VERSION     DBNHBY010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_433199"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..2459
                     /locus_tag="AC16HB_11090"
     CDS             159..2459
                     /locus_tag="AC16HB_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR5607380.1"
                     /translation="MAQPDNTEFRTAALEYHEQPRPGKISVTPTKQLSNQRDLALAYS
                     PGVAAASEAIHEDPAAVFRYTARGNLVGVITNGTAVLGLGPIGPLAAKPVMEGKGVLF
                     KKFAGIDVFDIEVNERDPDKLVEIIAALEPTFGGINLEDIKAPECFYIERKLRERMRI
                     PVFHDDQHGTAIIVGAAATNGLAIVGKSLSQVKLVCSGAGAAALACLDLLVELGMPRA
                     NIWVADIEGVVYDGRTVLMDAEKARYAQRTTARKLADIIDGADIFLGLSAGGVLKQEM
                     VAKMAARPLILALANPTPEILPEEVMAVRDDAIIATGRSDYPNQVNNVLCFPFIFRGA
                     LDVGSTTITKEMEVAAVQAIAQLAREEGSDIVASAYGATDFSFGPEYLIPKPFDPRLM
                     MRIAPAVAHAAMASGVAARPIADLEAYRSQLQQFVYHSGTFMKPIFDIARKAVASGGH
                     GRVVFAEGEEERVLRAAQVAIDEGLARPILVGRPAVIERRLEKHGLRMRTGTDFELVN
                     PESDPRYRDFWQTYHQMTARSGVTEQLARIEMRRRNTLIGAMLIHKGDADALICGTYG
                     TNDMHLAYVDRVIGRRPGAHVYAAMNILMLPGRQVALVDTHVNVNPSAEEVAEITIMA
                     AEELKRLGITPRTALLSHSNFGSSRAPSAEKMRRALQLVRDRMPDLAVDGEMHADSAI
                     DESIRRSIMPYSTLQGDANLLVCPDIDSANIAYNLLKTAAGNNVAIGPILLGAARPVH
                     IMTASATVRRIVNMTALAVLDANSAR"
     gene            2579..3490
                     /locus_tag="AC16HB_11095"
     CDS             2579..3490
                     /locus_tag="AC16HB_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5607381.1"
                     /translation="MLNSEKESSIRIAALSGFTDAIVCSIAAATSTSSLLLADALKTG
                     LEFMAVLLAWMSIRRITRGAGHEFEFGIGKLENLASLVIGSLMVLGVTIISYAAIRSL
                     IEPSHASGIGIYISLADQVVYGVINGYLMRRAAQAAKHENSPIMASQAKMFFAMFFGN
                     AFIFITVSLGLLLHNFGWSVYIDPLAALVVAATIVMAAIEVFSTSCYDLLDGALAEED
                     KLRIMRVLAENFDRYDMIYGVRSRRSGSHAFIEIYLGYDAQRRVGEVEREIEVLRNAV
                     ARLIRNSSVIVVLGPEPKNSTPALAAA"
BASE COUNT          645 a         1201 c         1189 g          614 t
ORIGIN      
        1 gacgcgccgc gctccacgag cgagtcggcg gccttcacca cctccatgac gtagaacggc
       61 tcgatttcgt tgagccgcgt tgccgttggc aacaaggttc ttcctcctcc gtgcttggcc
      121 ggcgtcataa tgcacgcccc cgaccccggc gaaccgcgat ggctcagccc gacaacaccg
      181 agtttcgcac cgccgcgctc gaataccacg agcagccacg acccgggaag atctcggtca
      241 cgccgaccaa gcaactaagc aaccagcgcg atctcgcgct ggcgtattcg cctggcgtgg
      301 cggcggcgag cgaagcgatt cacgaggacc cggcagcggt attccgttat accgcgcgcg
      361 gcaatctggt cggtgttatc accaacggca ccgcggtgct cgggctgggc ccgatcggcc
      421 cccttgccgc caagccggtg atggagggca agggcgtcct gttcaagaag ttcgccggta
      481 tcgacgtgtt cgacatcgag gtcaacgaac gcgatccgga caagctggtc gagatcatcg
      541 ccgcgctgga gccgaccttt ggcggcatca acctggagga catcaaggcg ccggagtgct
      601 tctacatcga gcgcaagttg cgcgagcgga tgaggattcc ggtcttccac gacgaccagc
      661 acgggaccgc gatcatcgtc ggggcggcgg caaccaatgg tctggcgatc gtcggcaagt
      721 cactgtcgca agtgaagctc gtgtgcagcg gcgccggtgc ggcggcgctg gcgtgcctcg
      781 acctgctggt cgagctggga atgccgcgcg ccaacatctg ggtcgccgac atcgagggcg
      841 tcgtgtacga cggccgcacg gtactgatgg atgccgagaa ggcgcgctac gcgcagcgga
      901 cgacggcgcg gaagctcgcg gacatcatcg acggtgccga cattttcctc ggtctgtcgg
      961 ccggcggcgt gctgaagcag gaaatggtcg caaagatggc cgcgcggccc ttgatcctcg
     1021 cgctcgccaa cccgacgccg gaaatccttc cggaggaggt gatggccgtg cgggacgacg
     1081 cgatcatcgc gaccggccgc tccgactatc cgaaccaggt gaacaacgtc ctctgtttcc
     1141 ctttcatctt ccgcggtgcg ctcgacgtcg gctcgacgac gatcaccaag gagatggaag
     1201 tcgccgcggt gcaggcgatc gcgcagctgg cgcgtgagga aggcagcgat atcgtcgcca
     1261 gcgcctacgg cgcgacggac ttcagcttcg gtcccgagta cctgattccc aagccgttcg
     1321 atccgagact gatgatgcgc atcgcgccgg cggttgccca tgccgcgatg gcgagcggcg
     1381 tggccgcgcg cccgatcgct gacctggagg cttaccgcag ccagcttcag caatttgtct
     1441 atcactccgg cacgttcatg aagccgatct tcgacatcgc caggaaggcg gtggcgagcg
     1501 gcgggcatgg gcgcgtggtg tttgccgagg gcgaggagga acgcgtgctg cgcgcggcac
     1561 aggtcgcaat cgacgagggg ctggccaggc cgatcctggt cggccgcccc gcggtgatcg
     1621 agcggcggct ggagaagcac ggtttgcgca tgcgcaccgg gacggacttc gaactggtca
     1681 atccggaatc cgacccgcgc tatcgcgact tctggcagac ctaccatcag atgaccgctc
     1741 gcagcggcgt cacggagcag ctggcacgaa tcgaaatgcg ccgacgtaac acgctgattg
     1801 gcgcgatgct gatacacaag ggcgacgccg acgccttgat ctgcggaacg tacggcacga
     1861 acgacatgca cctcgcgtac gtcgaccgcg tgatcggtcg gcggccgggc gcccacgtgt
     1921 acgcggccat gaacattctg atgctgcctg gtcgccaggt cgcgttagtc gacacgcacg
     1981 tgaacgtcaa cccgagcgcg gaggaagtgg ccgaaatcac gatcatggcc gcggaggaac
     2041 tgaagcgcct cggcatcacg ccgcgcacgg cactgttgtc gcattcgaac ttcggatcca
     2101 gccgcgcgcc gagcgcggag aaaatgcggc gcgcgctgca gctcgtccgc gaccgaatgc
     2161 ccgatctcgc cgtcgacggc gaaatgcacg ccgacagcgc gatcgacgaa tcgatccgcc
     2221 gctcgatcat gccgtactcg actttgcagg gcgacgccaa cctgctcgtc tgccccgaca
     2281 tcgactcggc caacatcgcc tacaacctgc tgaagacggc ggccggaaac aacgtcgcca
     2341 tcgggccgat cctgctcggc gccgctcgtc ccgtgcacat catgaccgcg tcggcaacgg
     2401 ttcgacgcat cgtcaatatg acggcgctgg cggtgctcga cgccaattcc gcgcgttagc
     2461 gcgacgttct tggcaataca tcggcgccgc gggcacaatg ccgggccgcc gcacctgcgg
     2521 ttgccattga atcgacaccg gagcgaacgg gcatggccgt gacgactggg ccgcgcacat
     2581 gctgaacagc gagaaggaat cgtcgatccg catcgcggcg ctgtcgggat tcaccgacgc
     2641 gatcgtctgc tcgatcgcgg cggcgacctc cacgtcgtcc ctgctgctgg ccgacgccct
     2701 gaagaccggg ctcgagttca tggcggtgct gctggcatgg atgtcgattc gacgcatcac
     2761 gcgcggcgcg ggccacgaat tcgagttcgg catcggcaag ctcgagaacc tcgccagcct
     2821 cgtgatcggc tcgctgatgg tgctgggcgt gacgatcatc tcgtacgccg cgatccgcag
     2881 ccttatcgaa ccatcgcatg cgagcggcat cggcatctac atcagcctgg cggaccaggt
     2941 cgtgtacggc gtgatcaacg gctacctgat gcggcgcgcc gcgcaggccg ccaagcacga
     3001 gaactcgccg atcatggcct cgcaggcgaa gatgttcttc gcgatgttct tcggcaacgc
     3061 gttcatcttc attaccgtct cgctcgggct gctgctgcac aacttcggct ggagcgtcta
     3121 catcgatccg ctggcggcgc tcgtggtcgc ggcgacgatc gtgatggcgg cgatcgaggt
     3181 gttctcgact tcctgctacg acctgctcga cggcgcgctg gccgaggagg acaagcttcg
     3241 catcatgcgc gtgctggcgg agaacttcga ccgttacgac atgatctacg gcgtgcgctc
     3301 gcgtcgctcg ggaagccacg ccttcatcga aatctacctc ggctacgatg cccagcgccg
     3361 cgtcggcgag gtggagcggg agatcgaagt gctccgcaac gcggtcgccc gtctgatccg
     3421 caacagctcg gtgatcgtcg tgctcggtcc cgagccgaag aattcgacgc cggcgctggc
     3481 cgccgcctga ttcgcactgc cgcgtcaccg cgatcggcct tcggccttgc gcacgtgcgc
     3541 ggatactcct aatctcgccg ttctttgcat tgccgccaag atcgatccgt gagcgagatt
     3601 ccaggtggga ccaccggttg ggacggccgc acgacgctgc tcgaccgcg
//
LOCUS       DBNHBY010000444         3724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1652602, whole genome shotgun sequence.
ACCESSION   DBNHBY010000444 DBNHBY010000000
VERSION     DBNHBY010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1652602"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="AC16HB_11100"
     CDS             <1..589
                     /locus_tag="AC16HB_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5607382.1"
                     /translation="QMAGLAEVPVLIRNVPDESALAMALIENIQREDLNALEQAQGLQ
                     RLIREFGFTHEQAAQSIGRSRSAASNLLRLLNLAAPVQEMLLGGQLDMGHARALLAVD
                     AATQVQLGHQIVVKGLSVREAEALVARAQRGDAGRSSMKAAHRSRDIERLEEELADAL
                     GATVRLNADAKGKGRLIIEFSNLDQLDGLVVRLKG"
     gene            713..1090
                     /locus_tag="AC16HB_11105"
     CDS             713..1090
                     /locus_tag="AC16HB_11105"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR5607383.1"
                     /translation="MSDRRAGLGRPIRAAIRRQAVVTAVLVVVAALWAGMHGAVSAAL
                     GGMISIGAGVVAAFIATRRPADSAGGILYGALRAEGVRVVLIILLLWLVLATYRDIVA
                     VAFFVSFAVTLLIFSSALFMRDE"
     gene            1109..1954
                     /gene="atpB"
                     /locus_tag="AC16HB_11110"
     CDS             1109..1954
                     /gene="atpB"
                     /locus_tag="AC16HB_11110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5607384.1"
                     /translation="MAAETELNPTSYIQHHLSFFHKPLGDGEFWVLHVDSLVTAAIVG
                     VLGLFFFWWVARGVTSGVPGRRQAFIELAVEFIDEQVKGIFHHASRDKFVAPAALTVF
                     VWVLLMNAMDFIPVDIASKALGAIGLHEWRLVPTADVNTTFALALSVWLLMIGFAIAV
                     KGPVGWIQELFCAPFGMVAPTLNPLKWVAGLLLATANILFNAIEYLSKPLSHSLRLFG
                     NMYAGEIIFLLLWMWAATGLVGTIFGSVLGLGWAIFHILIVVLQAYIFMMLTVVYIAL
                     AHEHH"
     gene            2005..2277
                     /gene="atpE"
                     /locus_tag="AC16HB_11115"
     CDS             2005..2277
                     /gene="atpE"
                     /locus_tag="AC16HB_11115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR5607385.1"
                     /translation="MEKLQLLAMIQAYTGIGIGLMIGVGAAGACIGVGIMCSRFLEAA
                     ARQPELTNSLQAKVFLLLGLIDASFIIGVGLAMMFAFANPLLAVVR"
     gene            2300..2770
                     /locus_tag="AC16HB_11120"
     CDS             2300..2770
                     /locus_tag="AC16HB_11120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR5607386.1"
                     /translation="MNINFTLFAQAIVFALFIWFTAKFIWPPILRAMDDRRKRIADGL
                     AAAEQGTKSLDEAKHRIAQLDADARGRAQALIAETERHGQQIIEEARAQAKVEADRVI
                     ANARAEAEQAMQRAKESLRDQVAALAVAGAEQILKKEVNAQVHADLLAQLKGKL"
     gene            2774..3316
                     /locus_tag="AC16HB_11125"
     CDS             2774..3316
                     /locus_tag="AC16HB_11125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR5607387.1"
                     /translation="MAELSTIARPYAEALFSAAAAAGADARESWGALVAELAGLTAHP
                     QVAQVVADPNLDSARTFDLLAGMFKTSLPAPVANFLRTLIDNGRLATLPEIARQYRLL
                     KNEADGVADCLIETAFPISDAQVNELLGLLTKNFGLRLKPEVKVDAALIGGVRVTVGD
                     HVLDTSVKTQLARMQAALTA"
     gene            3333..>3724
                     /locus_tag="AC16HB_11130"
     CDS             3333..>3724
                     /locus_tag="AC16HB_11130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5607388.1"
                     /translation="MQLNPSEISELLKSRIQGLGISTDIRTQGTVVSVTDGICRVHGL
                     SDVMQGEMLEFPGNTFGLALNLERDSVGAVVLGAYEHISEGDTVKSTGRILEVPVGPE
                     LIGRVVNSLGQPIDGKGPINAKMTDVIE"
BASE COUNT          634 a         1125 c         1261 g          704 t
ORIGIN      
        1 gcagatggcg ggtctcgccg aggtgccggt gctgatccgc aatgtgcccg acgaatcggc
       61 gctggcgatg gcgttgatcg agaacatcca gcgcgaggac ctgaatgcgc tggagcaggc
      121 gcaggggttg cagcgcctga tccgcgagtt cggcttcacg cacgagcagg ctgcgcagtc
      181 gatcggacgc tcacgcagcg cggcgtcgaa tcttctgcgg ctgctcaacc tcgccgcgcc
      241 tgtgcaggaa atgctgctgg gcggccaact cgacatgggt cacgcgcgtg cgctgctggc
      301 ggtcgacgcc gcgacgcagg tgcaactcgg ccatcagatc gtcgtcaagg ggctgtccgt
      361 gcgcgaggcc gaggcgctgg tcgcgcgcgc tcaacgcggc gacgcgggac ggtcctccat
      421 gaaggccgcg caccgcagcc gcgacatcga gcggctggaa gaagaactgg cggatgcact
      481 cggggcaaca gtgaggctga acgcggacgc caaggggaag ggccgcctga tcatcgagtt
      541 ttccaaccta gatcaactcg acgggctggt ggtgcggcta aaaggctaac cgcctgcaag
      601 agtatcgaca gcggtgcgcg ccgtcgttca ttgccggtgt ccgcgtttgc ggcgcgcgca
      661 gcggtttcga tacaattgcg ggctccgcag gaagtcgagt taagtcgggt ccatgagtga
      721 tcggcgtgct ggcttgggac gaccgatccg ggccgcgata cgtcggcaag ccgtggtcac
      781 ggcggttctc gtcgtcgtcg cggcattgtg ggcgggtatg catggcgccg tctcggcggc
      841 gttgggggga atgatcagta tcggtgccgg agtggtcgcg gctttcatcg cgacgcgtcg
      901 accggcggac tcggcagggg gcatcctgta cggcgcgctg cgcgccgagg gtgtacgggt
      961 cgtgctgatc atcctgctgc tttggctggt gctcgcgacg tatcgggaca tcgtcgcggt
     1021 tgcattcttc gtgtcctttg ctgtgacgct tctgatcttc tcgtcggcgc tgttcatgcg
     1081 ggatgaataa acgatcgatg taacgacgat ggcagccgaa accgaactga accccaccag
     1141 ctacatccag catcacctga gcttcttcca caaaccgctc ggcgacggcg aattctgggt
     1201 gctgcacgtc gattcgctgg tgaccgcggc gatcgtcggg gtgcttggcc tgttcttctt
     1261 ctggtgggtg gcgcgcggcg tgacttccgg cgtgcccggt cgccggcagg ctttcattga
     1321 actcgccgtc gagttcatcg acgagcaggt caagggcatc ttccatcacg ccagccgaga
     1381 caaattcgtc gcgccggcgg cgctcacggt gttcgtgtgg gtgttgctga tgaatgcgat
     1441 ggacttcatt ccggtcgaca tcgcctcgaa ggcactcggc gcgatcggct tgcatgaatg
     1501 gcgcctcgtg ccgacggccg acgtcaacac gacgttcgcg cttgcgctgt cggtgtggct
     1561 cctgatgatc ggcttcgcga tcgcggtcaa gggcccggtc ggctggattc aggagctttt
     1621 ctgcgccccg ttcggcatgg tggcgcccac gctgaacccg ctgaaatggg tggccggact
     1681 gttgctcgcc acggcgaata tcctgttcaa cgcgatcgag tacctgtcca agccgctgtc
     1741 gcactcgctg cggttgttcg ggaacatgta cgcgggcgag atcattttcc tgttgctgtg
     1801 gatgtgggcg gcgaccgggc tggtggggac gattttcggc tcggttctgg gtctgggctg
     1861 ggcgatcttc cacatcctga tcgtcgtgct gcaggcttac atcttcatga tgctgacggt
     1921 tgtctacatc gcattggcgc acgagcacca ctgagtttca aaagcagaga ttcttttaat
     1981 tcccaaggca gaaaggtaag agcaatggaa aagctgcaac tcctcgcgat gatccaggcc
     2041 tacaccggca tcggcatcgg tctgatgatc ggcgtcggcg ccgccggcgc gtgcatcggc
     2101 gtcggcatca tgtgcagccg atttctcgaa gcggcggcgc gccagcccga gctgaccaac
     2161 agcctgcagg caaaggtgtt cctgctgctt ggcctgatcg acgcgtcgtt catcatcggc
     2221 gtcggcctgg cgatgatgtt cgcattcgcg aatcctctgc tcgccgtcgt ccgctagtgc
     2281 gccgggccgc ggaggcggta tgaacatcaa tttcacgctg ttcgcgcagg cgatcgtatt
     2341 tgcactcttc atttggttca cggccaagtt catctggccg ccgatcctgc gcgccatgga
     2401 tgaccgacgc aaaaggatcg ccgacggcct ggccgccgcg gaacagggca cgaagagcct
     2461 cgacgaggcc aagcaccgga tcgcgcagct cgacgccgat gcgcgcgggc gcgcgcaggc
     2521 gctgatcgcc gagaccgagc ggcacgggca gcagatcatc gaggaggcgc gcgcccaggc
     2581 caaggtcgag gccgaccgtg tgattgccaa tgcccgtgcc gaggccgaac aggcgatgca
     2641 gcgcgcgaag gagtcgttgc gcgatcaggt cgccgcgctt gccgttgccg gtgccgagca
     2701 gatcctcaag aaggaagtca atgcgcaggt tcacgccgac ctgctggcgc aactgaaggg
     2761 aaagctgtag gccatggcgg agctgtccac cattgccagg ccttatgcgg aggcgctttt
     2821 ttctgcggct gccgctgcgg gcgccgatgc gcgcgagtcg tggggcgcgc tggtcgcgga
     2881 actggccggg ctcaccgcgc atccgcaggt cgcgcaggtc gtcgccgatc cgaatctgga
     2941 cagcgcacgt acgttcgacc tgctcgcggg aatgttcaag accagcctgc ccgcgccggt
     3001 cgcgaacttc ctgcgcacgc tgatcgataa cggccggctc gcgacgctgc cggagatcgc
     3061 gcgccagtac cgtctgctga agaacgaagc cgacggcgtc gccgattgcc tgatcgagac
     3121 cgcattcccg atcagcgatg cgcaggtgaa tgaactgctc ggtctgctga cgaagaattt
     3181 cggcctgcgc ctgaagcccg aagtcaaggt cgatgccgcc ctgatcggcg gggtgcgcgt
     3241 gaccgttggc gatcacgtgc tcgacacctc ggtcaagact cagcttgcgc gcatgcaggc
     3301 tgcgttgacc gcctgatcac tggagttgaa agatgcaact caatccgtct gaaatcagcg
     3361 aactgctgaa gagccgcatc cagggcctcg gcatttcaac cgatatccgc acccagggca
     3421 ccgtcgtgtc ggtgaccgac ggcatctgcc gcgtgcacgg cctgtcggac gtcatgcagg
     3481 gcgagatgct ggagtttccc ggcaacacgt tcgggctggc gctgaacctc gagcgcgact
     3541 cggtcggcgc ggtcgtgctg ggagcgtacg agcacatcag cgaaggcgac acggtcaagt
     3601 cgaccggccg cattctcgaa gtgccggtcg gtccggaact gatcggccgt gtcgtgaatt
     3661 cgctcggcca gccgatcgac ggcaagggac cgatcaacgc caagatgacg gacgtgatcg
     3721 agaa
//
LOCUS       DBNHBY010000445         2100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_433590, whole genome shotgun sequence.
ACCESSION   DBNHBY010000445 DBNHBY010000000
VERSION     DBNHBY010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_433590"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /gene="gshA"
                     /locus_tag="AC16HB_11135"
     CDS             <1..1154
                     /gene="gshA"
                     /locus_tag="AC16HB_11135"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR5607389.1"
                     /translation="RYGRVMQAIAGVHFNYSFPDAFWPVLADVLKGHDGQDFRSEAYF
                     ALLRSYRRHGWIVLYLFGNSPALCPSFLQGRQVDGLEPLEPGSLYAPYATSLRMSDLG
                     YRNKSQAGLNVSVNSLEHYVRDLTAAISTPSPEYQKIGVKVGDEWRQLNANVLQIENE
                     YYSFIRPKRVTQSGERPTKALQRAGVQYVEMRSLDVSAFDPVGVNQNKLRFLEAFAAF
                     CVLRESRAIESWEQSDLDGNHATVAREGRRPGIMLRREGRDVALRDWAMEIVDSMRGV
                     CELLDEGDPQRPYSAALEVQEEKVRDASLTPSARSLAEMRTNEESFFNFALRMSRTHQ
                     GYFRELYPPNPARQAEFAGEAVESLEKQRQIEAADRISFDEYIARWFAR"
     gene            complement(1663..>2100)
                     /locus_tag="AC16HB_11140"
     CDS             complement(1663..>2100)
                     /locus_tag="AC16HB_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HIR5607390.1"
                     /translation="EKLPDGHESLMGTIGAVALDESGNLAAATSTGGMTAKRWGRVGD
                     SPIIGAGTYAANDCCAVSATGHGEFFIRATVAHEIAALVRYAGKTVEQAADIVVHQQL
                     KRLGGDGGVIAIGRDGSIAMPFNSRGMLRGSIDARGVLYTGIL"
BASE COUNT          362 a          699 c          703 g          336 t
ORIGIN      
        1 accgctacgg ccgcgtcatg caggcgatcg ccggggtgca cttcaactat tccttccctg
       61 acgcgttctg gcccgtgctg gcggacgtcc tcaagggcca cgacgggcag gacttccgtt
      121 ccgaggcgta cttcgccctg ctgcgcagct atcgcaggca cggctggatc gtgctatacc
      181 tgttcggcaa ctcgccggcg ctgtgtccgt cgttcctgca gggccgacag gtcgacggcc
      241 tcgagccgct ggagccgggc agcctgtacg cgccgtacgc aacgtcgctg cgcatgagcg
      301 acctcggcta ccgcaacaag agccaggccg gcctcaacgt ctcggtcaac agcctcgagc
      361 actacgtgcg cgacctgacg gccgccatca gcacgcccag cccggagtac cagaagatcg
      421 gcgtcaaagt cggcgacgag tggcgccagc tcaacgccaa cgtcctgcag atcgaaaacg
      481 agtactacag cttcatccgc ccgaagcgcg tcacgcagag cggcgagcgc ccgaccaaag
      541 cgctgcagcg cgcgggcgtg cagtacgtcg agatgcggtc gctggacgtg agcgcgttcg
      601 acccggtggg cgtcaaccag aacaagctgc gcttcctcga ggcgttcgcg gcgttctgcg
      661 tactgcgcga aagtcgcgcg atcgagagct gggagcagtc cgatctggac ggcaaccacg
      721 cgacggtggc gcgcgaaggg cggcgtcccg gcattatgtt acgtcgcgaa ggacgcgacg
      781 ttgcattgcg cgactgggcg atggagatcg tcgactcgat gcgcggcgtg tgcgagctgc
      841 tcgatgaagg cgatccgcag cgtccgtact cggcggcgct cgaggtgcag gaagagaagg
      901 tgcgcgatgc gtcgctgacg ccctcggcac gctcgctggc cgagatgcgc acgaacgagg
      961 agtcgttctt caatttcgcg ctgcgcatgt cgcgcacgca tcaaggctat ttccgcgagt
     1021 tatacccgcc gaatccggcg cgccaggcgg aattcgcggg cgaggccgtc gaatcgctcg
     1081 aaaaacagcg gcagatcgag gctgcggacc gcatctcgtt cgacgaatat atcgcgcgct
     1141 ggttcgcgcg gtgacctgca ttccgctgct gttcgcgcgc gcatccgggc tcggagtcgg
     1201 tcgctttcgt acgcgcacgt aggaatccgc tgaaaccgag ggcgtcgcct tcgccgaatc
     1261 gcgctccgcg aaaaaatatc tgggttgtta ccttcttgtc acaaaggcgg cgctacaatc
     1321 cgcgccgatg cgggatgcaa cgaatgatcg gggcgtaggc aagggctcgc gccacgcagg
     1381 gcggtcgaag atgactaacg tgacgactac gatgggcgcc gtgcccgcgg catgggccgc
     1441 gaacactggc cgctcgcagg caccggctgc agcgcaggga acgcgcgctc ctggtccggt
     1501 cgaaccgaat ttcgccacgg cactgcaagg tgcccgtagc caggcctggg atccacacga
     1561 ggtgtggctg acgcgcgtca agcagccgcg cgaccagcgc ggcgatcgct gaccctcacc
     1621 cggcctgcgg ccaccctctt ccgcgagcgg gagaggggag gcctacagta ttcccgtata
     1681 gagcacgccg cgcgcgtcga tcgacccgcg cagcatcccg cgcgagttga acggcatggc
     1741 aatcgagccg tcgcggccga tcgcaatgac gccgccgtcg ccgcccagcc gcttgagctg
     1801 ctggtgcacg acgatgtcgg ccgcttgctc cacggtcttg cccgcatagc gcaccagcgc
     1861 ggcgatttcg tgcgcgacgg tcgcgcggat aaagaactcg ccatgacccg tggccgagac
     1921 tgcgcagcag tcgttggccg cgtaggtgcc cgcgccgatg atcggcgaat caccgacgcg
     1981 tccccagcgc ttggccgtca tgccgcccgt cgaggtggcg gcggccaggt tgcctgactc
     2041 gtcgagtgcc acggcgccga tcgtgcccat cagcgattcg tgaccgtcgg gcagcttttc
//
LOCUS       DBNHBY010000446         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_772879, whole genome shotgun sequence.
ACCESSION   DBNHBY010000446 DBNHBY010000000
VERSION     DBNHBY010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_772879"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="AC16HB_11145"
     CDS             <1..317
                     /locus_tag="AC16HB_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5607391.1"
                     /translation="IFEHQAVNFRLADMATKIEAARQLVLNAASLKDQGVPCLKEAAM
                     AKLFASEMAERVCSDAIQVHGGYGYVTDFPVERIWRDVRVCQIYEGTSDIQRIIIGRA
                     LA"
     gene            427..1155
                     /locus_tag="AC16HB_11150"
     CDS             427..1155
                     /locus_tag="AC16HB_11150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607392.1"
                     /translation="MAARPRYPRALALLLARELELPLDAPIADIGSGTGISCEPFLAA
                     GFEVIGVEPNDAMRAAAERELPRYTRFRSVKGTAEATTLLAGSVDLAIAGQAFHWFDH
                     ARFRAELLRMLKPGGALALFWNSRVHDASEFMRGYNDLLLDHCPEYRAKWKGDDVGRK
                     HAPAMELVFGDRHWREAALDNAQTLDRAGLIARVESDSYAPKPGDPSHPPMVAALHDL
                     FDRCAQDGRVQMVYRTRIFFGRPR"
     gene            complement(1162..>1575)
                     /locus_tag="AC16HB_11155"
     CDS             complement(1162..>1575)
                     /locus_tag="AC16HB_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5607393.1"
                     /translation="RTEHFYRDVLGFRLSDYIDTEFMGNPLHAVFLHVNPRHHSLAFA
                     SLGMPKRLHHMMLEVNSIDDVGAAFYRAQDLGVPIALTLGRHPNDGMISFYGVTPSGF
                     LFELGVDGREVDDRTWEVKTYHAVSDWGHRPVAPA"
BASE COUNT          266 a          523 c          521 g          265 t
ORIGIN      
        1 cgatcttcga acaccaggcg gtcaacttcc gcctcgccga catggcgaca aagatcgaag
       61 cggcgcgaca actggtgctc aatgccgcca gtctcaagga ccagggcgta ccgtgcctga
      121 aggaagcggc gatggcgaag ctcttcgcct ccgagatggc ggagcgcgtc tgctcggacg
      181 cgatccaggt tcacggcggc tacggctacg tgaccgactt cccggtcgag cgcatctggc
      241 gcgatgtgcg cgtgtgccag atctacgaag gcaccagcga catccagcgc atcatcatcg
      301 ggcgcgcgct ggcgtgaaat gcccctgctt cccttcaaat cccccctgcc ccccctttcc
      361 aaaggggggt gagcgatgag ccttgcggac ccgacgcaca ggttctcaga ttgcgcggat
      421 gcgtacgtgg cggcaaggcc gcggtatccg cgagcgcttg cgttgctgct ggcgcgggag
      481 ctggagctgc cgctggatgc gccgatcgcc gacatcggct cgggcaccgg catctcgtgc
      541 gagccttttc tcgccgcagg gttcgaggtg atcggcgttg agccgaacga cgcgatgcgc
      601 gcggcggccg aacgcgagct cccccgctac acgcgctttc gcagcgtcaa aggcacggcc
      661 gaggccacca cgttgttggc gggctcggtc gatctcgcga tcgcgggcca ggcgttccat
      721 tggttcgacc acgcacgctt tcgcgccgag ttgctgcgga tgctgaagcc cggcggcgcg
      781 ctggcgctgt tctggaattc gcgcgtgcac gacgcttcag agttcatgcg cggctacaac
      841 gacctgctgc tcgaccactg tcccgagtac cgcgcgaagt ggaagggcga cgacgtcggc
      901 cgcaagcacg cgcccgcgat ggaactcgtg ttcggcgacc gccactggcg cgaggccgcg
      961 ctcgacaatg cgcagacgct cgatcgcgcc ggcctgatcg cgcgcgtcga atccgactcc
     1021 tatgcgccga agcccggcga tccgtcgcat ccaccgatgg tcgccgcgct gcacgacctc
     1081 ttcgatcgct gcgcgcagga cggtcgcgtg cagatggtct atcgcacgcg gatcttcttc
     1141 ggccggccgc gttagcgcgt gctaggccgg cgccaccggc cgatgtcccc agtcgctcac
     1201 ggcgtgatag gtcttgacct cccaggttcg atcgtcaacc tcgcggccat ccacgcccaa
     1261 ctcaaagagg aagccggagg gcgtgacccc gtaaaacgag atcatgccgt cgttcgggtg
     1321 gcgtccgagc gtgagagcga tcggcacgcc cagatcctgc gcgcgataga aggccgcgcc
     1381 gacgtcgtcg atcgagttca cttcgagcat catgtgatgc agccgcttgg gcatgccgag
     1441 agacgcgaac gcgagcgaat gatggcgcgg gttcacgtgc agaaacaccg cgtgcagcgg
     1501 attccccatg aattcggtgt cgatgtagtc gctcaggcgg aagccgagca cgtcgcggta
     1561 gaagtgctcg gtcct
//
LOCUS       DBNHBY010000447         3233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_366954, whole genome shotgun sequence.
ACCESSION   DBNHBY010000447 DBNHBY010000000
VERSION     DBNHBY010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_366954"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC16HB_11160"
     CDS             <1..507
                     /locus_tag="AC16HB_11160"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR5607394.1"
                     /translation="LHGRTRACGFTGAAEYDTITAVKAAVSIPVVANGDIDTPERARR
                     VLEITGADAIMIGRAAQGRPWIFREIDHYLRTGDYLPPLRVEEVRPLLLAHLDDHYAF
                     YGREAGVRTARKHIIWYTQRLEGGTAFCARMNAIDTIEAQRRAVDEFMCEFGARHERL
                     VEAAATLH"
     gene            619..840
                     /locus_tag="AC16HB_11165"
     CDS             619..840
                     /locus_tag="AC16HB_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5607395.1"
                     /translation="MEECIVRSLTQYFDDLGGAKPHALHEMVMVAVERPLLKFALDRA
                     GGNQSAAAELLGINRNTLHKKLCGYGLAK"
     gene            837..2387
                     /gene="purH"
                     /locus_tag="AC16HB_11170"
     CDS             837..2387
                     /gene="purH"
                     /locus_tag="AC16HB_11170"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5607396.1"
                     /translation="MRNALIGVSDKTGIVEFARALAALEFRLLSTGGTAKLLRDNGIA
                     VTEVADYTGFPEILDGRVKTLHPRIHAGLLARRDLPAHVATLKEHRIDPIDLLVVNLY
                     PFEQTVARPDCTFDDAIENIDIGGPAMLRAAAKNHGSVTVVVDPVDYARVNEELKAHG
                     AVGAALRFELAKKVFAHTARYDGAISNYLSALDDDHRRGEFPQTLTRQWVKVQQMRYG
                     ENPHQSAAFYRERIVGAGLLAGYTQLQGKELSYNNIADADAAWECVRSFAETACVIVK
                     HANPCGVAVGSDVASAYTKAFQTDPTSAFGGIIAFNRTVDAECAQAVAKQFVEVVIAP
                     AFDDKARAILKAKQNVRLLTVEPGGAGNDFDFKRVGGGLLVQSPDGRQVQRSELRVVT
                     KLAPTEAQLDDLTFAWRVAKFVNSNTIVFARDGMTLGVGAGQMSRIDSARIASIKAKE
                     AGLTLVGSAAASDAFFPFRDGLDVVVDAGANCVIQPGGSVRDDEVIAAANERGVAMVF
                     TGTRHFRH"
     gene            2418..2984
                     /gene="ruvC"
                     /locus_tag="AC16HB_11175"
     CDS             2418..2984
                     /gene="ruvC"
                     /locus_tag="AC16HB_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR5607397.1"
                     /translation="MQLPKRILGIDPGLNRTGWGAIESIGGRLRCIDSGVIRVPPGDL
                     PQRLGVILAELGRVIVSVAPTCACIEKVFVNVNPQSTLLLGQARGAAICAAVAAGLSV
                     REHTALQIKHAVVGYGKASKEQVQMMVQRLLVLGAVPPTDAADALACAICDAHAERLL
                     LAGGVLSSSARARRSRSAWRAIAARGGA"
     gene            2981..>3233
                     /gene="ruvA"
                     /locus_tag="AC16HB_11180"
     CDS             2981..>3233
                     /gene="ruvA"
                     /locus_tag="AC16HB_11180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR5607398.1"
                     /translation="MIGRIEGRLLEKNPPSVLVDTNGVGYEIDVPMSTFYNLPALGER
                     IVLLTHLTVREDAHLLYGFGTAAERDAFRELIKISGIGSR"
BASE COUNT          558 a         1081 c         1118 g          476 t
ORIGIN      
        1 ctgcacgggc gcacgcgcgc ctgcggcttc accggcgcgg cggagtacga cacgatcacc
       61 gcggtgaagg cagcggtgtc gattccggtc gtcgccaacg gcgatatcga cacgcccgag
      121 cgcgcgcgcc gcgtgctcga gatcacgggc gccgacgcga tcatgatcgg tcgtgccgcg
      181 cagggccggc cgtggatctt ccgcgagatc gaccactatc tgcgcaccgg tgactatctg
      241 ccgccgctgc gcgtggaaga ggtgcggccg ctgctgctgg cgcatctcga cgatcactac
      301 gcgttctacg gccgcgaggc cggtgtgagg accgcgcgca aacacatcat ctggtacacg
      361 cagcgactgg aaggcggcac ggcattctgc gcgcgcatga acgcgatcga cacgatcgag
      421 gcgcagcgcc gcgcggtcga cgagttcatg tgcgagttcg gcgcacgcca cgagcggctg
      481 gtcgaggcgg cggcgacgct gcactaaaaa tttcgaggag acacgatgaa aaacagcgca
      541 ctgcgcacac ccgtccagcc cgcttccggg gagagcccgg ctgccgccaa cgcgcgcgcc
      601 gcacggcgcg gcgagacgat cgaggaatgc atcgtgcggt cgctcacgca atacttcgat
      661 gacctcggtg gcgccaagcc ccacgcgctg cacgagatgg tcatggtggc ggtcgagcgc
      721 ccactgctga agttcgcgct cgacagggcg ggcggcaatc agtctgccgc ggccgaactg
      781 ctcggcatca accgcaacac gctgcacaag aagctctgcg gctacggact ggcgaaatga
      841 ggaatgcact gatcggcgtc tccgacaaga ccggcatcgt cgagttcgcg cgcgcgctcg
      901 ccgcgctcga gttccggctg ctgtcgacgg gcggcaccgc gaagctcctg cgcgacaacg
      961 gcatcgcggt gaccgaggtg gccgattaca ccggttttcc ggagatcctc gatggccgag
     1021 tgaagacgct gcacccgcgc atccacgccg ggctgctggc gcggcgcgat ctgcccgcgc
     1081 acgtggcgac gctgaaggag caccgcatcg atccgatcga cctgctggtg gtcaacctgt
     1141 atccgttcga gcagacggtc gcgcgacccg actgtacgtt tgacgatgcg atcgagaaca
     1201 tcgacatcgg cggaccggcg atgctgcgcg cggccgcgaa gaatcatggt tcggtcaccg
     1261 tcgtcgtcga cccggtcgac tacgcgcgcg tgaacgaaga gctcaaggcc cacggcgcgg
     1321 tcggcgccgc gctgcgattc gaactggcaa agaaggtgtt cgcgcacacc gcgcggtacg
     1381 acggcgcgat cagcaactac ctgtcggcgc tcgacgatga ccaccgccgc ggcgaattcc
     1441 cgcagacgct gacgcggcaa tgggtcaaag tgcagcagat gcgctacggc gagaacccgc
     1501 accagagcgc ggcgttctat cgcgaacgca tcgttggcgc gggcctgctg gcgggctaca
     1561 cgcagctgca gggcaaggaa ctgtcctaca acaacatcgc cgatgccgac gcggcctggg
     1621 agtgcgtacg ctcattcgcc gagaccgcgt gcgtgatcgt caagcatgcg aatccgtgcg
     1681 gcgtcgccgt cggatccgat gtcgcctcgg cctacaccaa ggcgttccag accgacccca
     1741 cgtcggcctt cggcggcatc atcgcgttca accgcacggt cgacgccgaa tgcgcgcagg
     1801 ccgtggcgaa gcaattcgtc gaggttgtga tcgcgccggc gttcgacgat aaggcgcgcg
     1861 cgatcctcaa agccaagcag aacgtgcggc tgctgaccgt ggaaccgggc ggcgccggca
     1921 acgatttcga cttcaaacgc gtcggcggcg ggctgctggt gcagtcgccc gacggcaggc
     1981 aggtgcaacg ctccgagctg cgcgtggtga cgaagctcgc gccgaccgag gcacaactcg
     2041 acgacctgac gttcgcctgg cgagtggcca agttcgtcaa ttcgaacacg atcgtgttcg
     2101 cgcgcgacgg catgacgctc ggggtcggcg ccgggcagat gagccgcatc gattccgcgc
     2161 gcatcgcgtc gatcaaggcg aaggaagccg ggctgacact cgtcggctcg gcagcggcgt
     2221 cggatgcgtt cttcccgttc cgcgatggtc tcgacgtcgt ggtcgacgcc ggcgctaact
     2281 gcgtgatcca gcccggcggc agcgtgcgcg acgacgaggt gatcgccgcc gcgaacgagc
     2341 gcggcgtcgc gatggtcttc accgggacgc ggcatttccg ccactgaaga cccgttggca
     2401 cgtggcagaa tcgccgcgtg cagctaccca aacgcatcct gggcatcgat ccgggtctca
     2461 accgcaccgg ctggggcgcg atcgagagca tcggcggacg gctgcgctgc atcgacagcg
     2521 gcgtgatacg cgtgccgccg ggcgacctgc cgcagcggct cggcgtgatc ctggccgaac
     2581 tcgggcgcgt gatcgtgagc gtggcgccga cgtgcgcctg catcgagaag gtgttcgtca
     2641 acgtcaatcc gcaatcgacg ctgctgctgg gccaagcgcg cggcgccgcg atctgcgcgg
     2701 ccgtcgctgc aggcctctcg gtgcgcgagc acacggcgct gcagatcaag catgccgtgg
     2761 tcggctacgg caaggcgagc aaggaacaag tccagatgat ggtgcagcgg ctgctcgtgc
     2821 tcggcgccgt gccgccgacc gacgccgccg acgcgctggc gtgcgcgatc tgcgatgcac
     2881 acgccgaacg cctgctgctc gccggcggcg tactgtcgag cagcgcgcgt gcgcggcggt
     2941 cgcgcagcgc gtggcgcgcg atcgccgccc ggggtggcgc gtgatcggcc gtatcgaagg
     3001 ccggctgctg gaaaagaacc cgccgtcggt actggtcgac acgaacggcg tcggctacga
     3061 gatcgacgtg ccgatgagca cgttctacaa cctgcccgcg ctcggtgagc gcatcgtgct
     3121 gctgacgcac ctgacggtcc gcgaggacgc gcacctgctc tacggcttcg gcacggccgc
     3181 cgagcgcgac gcatttcgcg aactgatcaa gatctccggc atcggcagtc gca
//
LOCUS       DBNHBY010000448         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1854842, whole genome shotgun sequence.
ACCESSION   DBNHBY010000448 DBNHBY010000000
VERSION     DBNHBY010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1854842"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2093
                     /locus_tag="AC16HB_11185"
     CDS             <1..>2093
                     /locus_tag="AC16HB_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5607399.1"
                     /translation="KLKRGSAAELEETIAAVDAPLASATATNTQTRKELKGDLDAILN
                     KALKKDPGQRYPSVDAFAQDLERYLRGEPVLARPDSTSYRLRKFVGRNRVAVGASLAV
                     MLAVVSGGSVALWQAQQARGEAARAQAVQAFLLDLFRTNSADQPDPQRARQTTARQLL
                     DIGAEKLATSLADQPASRFILAETLGDLYAELGLWPEAARMTAERLALARKLYGSNDL
                     RVAEALVAHSHSLFALNRDPAVEVAPLLDEAERLLDTAGEHMSTRRARLHTFAGRLWM
                     NPSPARARSHVERSVAIYRREHPRDPGFVDALDALSLTLVRQGEFEAAAVRIEEAVAL
                     ARAQGLSAFHLAPLLRRAGMIHAFAGRLDSADRLLREALEMTERVHGEHSVHTAQARL
                     SLARHLASTGHTAEAREHADRALADASGRHGGDEPWWLQEVRRQVFEVYWAQGDQIAC
                     RDLIVQAFSEIGSATPDSFEHADLLIERAAVETAEGDYDSARRSTDAARAMTDRLGMG
                     PQALLRYNLDLTDAQLRVAAGDTSGALQQLDAPALRAAKDTDKRLLLAATRAEALAAA
                     GQAEAARRLADEARAELERWPRRDQHADVEALVQLRWGVLRSADDDCQSALPALQRST
                     MLFRRIHVPLSPQRLNAELAHALCLARLERKREAQSVLIEVEASVARLGSLAKPLRAP
                     LAELRAQLRGADWRDG"
BASE COUNT          356 a          701 c          726 g          310 t
ORIGIN      
        1 aagctgaagc gcggcagcgc ggccgagctg gaggagacca tcgccgccgt cgacgcgccg
       61 ctggccagtg ccaccgccac caacacccaa accaggaaag aactgaaggg cgacctggat
      121 gcgatcctga acaaggcgct gaagaaggat cccggccaac gctacccgag cgtcgacgcg
      181 ttcgcccaag atctggagcg gtatctgcgc ggcgagccgg tgctggcgcg gccggatagc
      241 accagctacc ggctgcgaaa gttcgtcggg cgcaaccgcg tggcggtcgg cgcctccctg
      301 gcggtgatgc tggcggtcgt gagtgggggc agcgtcgcac tttggcaggc gcagcaagcc
      361 cggggcgagg ccgcgcgagc gcaggctgtg caggcattcc tgcttgactt gtttcgaacc
      421 aactcggccg accagccgga tccgcagcgc gcgcgccaga cgacggcgcg acagttgctc
      481 gacatcggcg ccgagaagct tgcgacgtcg cttgctgacc agccggcatc gcgcttcatc
      541 ctggccgaga cgctgggcga cttgtatgcc gaactcggac tctggcccga agcggcgcgc
      601 atgaccgcgg agcgactggc gctggcgagg aaactgtacg gaagcaacga tctgcgtgtg
      661 gccgaggcgc tggtggcgca ctcgcacagc ctctttgcgc tgaatcgaga tccggcggtc
      721 gaagtcgcac cgttactcga cgaagccgaa cgactgctgg acacggccgg cgaacacatg
      781 agcacccgac gggcgcggct gcacacattc gctggcaggc tctggatgaa cccttcgccg
      841 gcacgcgcgc gcagccatgt cgagcgcagt gtcgcgatct accggaggga gcacccgcgc
      901 gacccgggct ttgtcgacgc actagacgcg ctgagcctca cgctggtgcg ccaaggggag
      961 ttcgaggctg cagccgtgcg tatcgaggag gcagttgcgc tcgcccgcgc tcaaggcctg
     1021 tcggcattcc acctcgcgcc gctgttgcgg cgtgccggca tgatccacgc ctttgccggc
     1081 cgtctggact cggcagacag actactgagg gaggcgctcg agatgaccga gcgcgtgcac
     1141 ggtgaacaca gcgtccacac cgcgcaggcg cggctgagcc tagcgcgtca tctggcgtcg
     1201 acggggcata ccgccgaagc gcgtgaacac gccgaccggg cgctggccga cgcgtctggg
     1261 cggcacggag gagatgagcc gtggtggttg caggaagttc gccgccaagt gttcgaggtc
     1321 tattgggcac agggcgatca gatcgcatgc cgcgatctga tcgtgcaagc tttcagcgag
     1381 atcggttcgg ccacaccgga ttcgtttgag cacgccgacc ttctaatcga gcgcgcggct
     1441 gtcgagaccg cggaaggcga ctacgactcg gcgcggcgca gtaccgatgc cgcgcgcgcc
     1501 atgaccgatc gactcggtat ggggccacag gctttgctgc gctacaacct cgacctcacc
     1561 gacgctcaac tgcgtgtggc cgccggcgac acgagcggcg cgttgcagca gctcgacgca
     1621 ccggccttgc gcgcagccaa ggacaccgac aagaggttgt tgctggcggc gacgcgcgcc
     1681 gaagcgcttg ccgccgcagg gcaagccgaa gccgcgcggc gcctggctga cgaagcccgc
     1741 gcagagctcg agcgctggcc gcgccgcgat cagcatgccg acgtcgaagc gctggtccag
     1801 ctccgctggg gtgttctgcg cagtgccgac gacgactgcc agagcgccct gcccgccctg
     1861 cagcgcagca ccatgctctt tcggcgtatc catgtgccgc ttagcccgca gcgcctaaac
     1921 gccgagctcg cgcatgcgct gtgtcttgcg cggctggagc gcaagcgcga agcgcaatcc
     1981 gtgctgatcg aggtcgaggc atcggttgcc cgccttggat cgcttgccaa acctcttcga
     2041 gccccgctgg ccgagcttcg tgctcagctc cgcggcgccg actggcgtga cgg
//
LOCUS       DBNHBY010000449         2386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1516969, whole genome shotgun sequence.
ACCESSION   DBNHBY010000449 DBNHBY010000000
VERSION     DBNHBY010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1516969"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..834
                     /locus_tag="AC16HB_11190"
     CDS             265..834
                     /locus_tag="AC16HB_11190"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5607400.1"
                     /translation="MITTPAQIAELQKSQIDALFALSHTVFNATERLVDLNLAATKAL
                     MEESADKTQELMGVKDVQELLAVSSGFAQPAMDKFVSYSRNVYSIASGASSDVSKIVE
                     TQIAESNKKVAELIEFASKSAPAGSEPAVSVFKSALAAANTAYDTFTKATKQAVDIVE
                     SNLSAATNATLKATGAANEVVKGKGKKAA"
     gene            complement(1072..1881)
                     /locus_tag="AC16HB_11195"
     CDS             complement(1072..1881)
                     /locus_tag="AC16HB_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR5607401.1"
                     /translation="MIAVPCFGLIIVGDEILSGKRADKHLARVIQILAARGLRLSWAH
                     YLGDDRAAITEALRRSFASEEIVFCCGGIGATPDDHTRQAAAAALGVPLRLHTEAAAL
                     ITQRCVEMAAEGRGTADMTTPENQQRLRMGEFPQNARIILNSYNRIPGFAAGRHYFLP
                     GFPVMAWPMIESILDNEYREHFHRVPLGERSMLVFETPESMVAPLMEEIEARFPGVKS
                     FSLPSVGDGRDGKLARRHIELGVKGPPELLDAAFADLRRRIEAIGAPFTVA"
     gene            2070..>2386
                     /locus_tag="AC16HB_11200"
     CDS             2070..>2386
                     /locus_tag="AC16HB_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR5607402.1"
                     /translation="MGFSELHRLQARILRSFAVAALALCAMLSASAASAQTADNADPP
                     GRVGRLSYLSGPVQLIDIRTGADEDATLNWPVTSGIRLATGRLGRAEVRIGSLAVWLD
                     DDS"
BASE COUNT          471 a          759 c          700 g          456 t
ORIGIN      
        1 atgctgtttc gcagcattcg tccccgcttt aattcgatat tgacagggcg gggcctgcgt
       61 aggccattga tttttgtcgg ttccgtcgtg aaactcgaat tcgagcatcg aatggtgcgc
      121 cgcaaagaaa tcgcttgaca ttcgcggaat gaaccataga attcattttg tgcgttgcac
      181 caaatgcgac gcaaacgaat caagcaaatt cggcgaatcc gccgatccct cgtgaaacag
      241 tgacccactg attaggagct acacatgatc accacccctg cccaaatcgc cgaactccag
      301 aaaagccaga tcgatgcgct gtttgcgctg tcgcacactg tcttcaacgc caccgagcgc
      361 ctggtcgacc tgaatctcgc cgccacgaag gcgctgatgg aagagtcagc cgacaagacc
      421 caggaactga tgggcgtcaa ggacgtgcag gaactgctgg cggtcagcag cggcttcgcg
      481 caaccggcga tggacaagtt tgtcagctat agccgcaacg tgtactcgat cgcgagcggt
      541 gcgtcgagcg acgtgtcgaa gatcgtcgaa acgcagatcg ccgagagcaa caagaaggtc
      601 gccgaactca tcgagttcgc gtccaagagc gccccggccg gttcggagcc cgcagtgtcg
      661 gttttcaaga gcgcactggc cgcggccaac accgcatacg acacgttcac caaggcgacc
      721 aagcaagccg tcgacatcgt cgagtcgaac ctttccgccg cgacgaacgc gacgttgaag
      781 gccaccggcg cggctaatga ggtcgtcaag ggcaaaggca agaaagcggc ctgagcatca
      841 aggagggtct gctagaagac acactcgaaa gaaccttgcg ttttgtcagt agtgactcgg
      901 cggactcgcc aacaatttga gttgtagtgg atcgagtgtc gagactgtga tctcgattct
      961 ttaagggttt tctccttggc accacttgtt tggaccccaa gtccaaaccc cttctgtctc
     1021 ctcctttggt ggtgctttca agcccggtcg caagaccggg ctttttttcg tctacgcgac
     1081 cgtgaacggg gcaccgatcg cttcgatgcg gcgcctcaaa tcggcaaacg cggcgtcgag
     1141 cagttcaggc ggtcctttca cgccaagctc gatgtgccgc cgggcgagtt tgccatcgcg
     1201 cccgtcaccg accgacggca ggctgaagga tttcacgccg gggaagcgcg cctcgatttc
     1261 ttccatcagc ggcgcgacca tcgactccgg agtctcgaac accagcatcg agcgctcccc
     1321 caatggaacg cgatgaaagt gctcgcggta ttcgttgtcg agaatcgatt cgatcatcgg
     1381 ccaggccatc accggaaatc cgggcaggaa gtaatggcgc ccggccgcaa agcccggtat
     1441 ccggttgtac gagttcagga tgattcgcgc gttctgcggg aactcgccca tcctcaggcg
     1501 ctgctgattc tccggcgtcg tcatgtcggc ggtgccgcgc ccttcggcgg ccatttccac
     1561 gcagcgctgg gtgatgaggg ccgccgcttc cgtgtgcaac cgtagcggaa cgccgagcgc
     1621 agcggccgcc gcctgacggg tgtgatcgtc gggcgtcgca ccgatcccgc cgcagcagaa
     1681 aacgatctcc tcgctcgcga agctgcgccg gagcgcttcg gtgatcgccg cgcggtcgtc
     1741 tcccaggtaa tgggcccacg aaagccgcaa tccgcgcgca gcgaggatct ggatcacgcg
     1801 cgccagatgt ttgtcggcgc gctttccgga aaggatctca tcgccgacga tgatcagccc
     1861 aaagcatggc accgcgatca tctcttgttc cgcacccccc gcgtgcgcgg aattctatcg
     1921 atccgtcgac gctaagctgc gcaggcaagc ctctcgcgcc gggatctgtg tccagttgtt
     1981 tcactcgtgc acgtaggccg ttccgcgcac gtttcatgac cgggttcaca cacggctgca
     2041 gggttgcaca cgccagaagg gtgatcacca tgggcttttc cgaactccat cgacttcaag
     2101 cgcgcatctt gcgcagtttc gccgtcgccg ctctcgcgct ttgcgcaatg ctgtccgcct
     2161 ctgcggcctc ggcccagacc gccgacaacg ccgacccgcc aggcagagtc ggccgcctca
     2221 gctacctgtc tggcccggtt cagctgatcg acatccgcac cggagccgac gaagacgcga
     2281 cattgaactg gccggtcact tccggcatcc ggttggcgac gggccggctc ggccgcgccg
     2341 aagtgcgcat cggctcactc gcggtctggc tcgacgacga ctccga
//
LOCUS       DBNHBY010000450         1541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_433838, whole genome shotgun sequence.
ACCESSION   DBNHBY010000450 DBNHBY010000000
VERSION     DBNHBY010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_433838"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1541
                     /locus_tag="AC16HB_11205"
     CDS             <1..>1541
                     /locus_tag="AC16HB_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5607403.1"
                     /translation="SLHEALRTLGDTSLPEPLERYGAAALADAAADGAVVRLRGAYND
                     ALESIGAEGIALLQQWPALARSATDEQYSYEVRGREIRGDNYVGTLSHNRVPKLAVPR
                     YREWGEQLSFLLRENLPGRFPYTAGVYPYRREEEDPTRMFAGEGGPERTNRRFHLLAS
                     GHGAARLSTAFDSTTLYGEDPAERPDIYGRIGNSGVSIATLDDMKKLYSGFDLCAPTT
                     SVSMTINGPAPMILAMFMNTAIDQRVERHLRETGQWDAAQRRIDELLKDRPRPAYRGD
                     LPPTHDGAGLGLLGVTGDQLVDAETYARIKAATLRQVRGTVQADILKEDQAQNTCIFS
                     TEFALRMMGDVQQYFTDHEVRNFYSVSISGYHIAEAGANPVSQLAFTLANGFTIVEYY
                     LARGMRIDDFAPNLSFFFSNGMDPEYAVIGRVARRIWARAMRDLYRAGPRSQMLKYHI
                     QTSGRSLHAREIQFNDIRTTLQALYALFDNCNSLHTNAYDEAITTPTEESVRRAVAIQ
                     LIISK"
BASE COUNT          269 a          536 c          513 g          223 t
ORIGIN      
        1 agcctgcacg aggcgttgcg cacgctgggg gacacgtcgc tgcccgagcc gctcgagcgt
       61 tacggagccg ctgcgctggc ggacgctgcg gccgacggtg ccgtggtccg gctgcgcggt
      121 gcctacaacg acgcgctcga atcgatcggc gcggaaggga tcgcgttgct gcagcagtgg
      181 cctgcgttgg cgcgctcggc caccgacgag cagtacagct acgaagtgcg cgggcgcgag
      241 atccgcggcg acaactacgt cggcacgctg agccacaacc gcgtgccgaa gctcgcggtg
      301 ccgcgctatc gcgaatgggg cgagcagctg tcgttccttc tgcgcgagaa cctgccgggc
      361 cgcttcccgt acaccgcggg cgtctacccg taccgccgcg aggaagaaga cccgacacgc
      421 atgttcgccg gcgaaggcgg gcccgagcgc acgaaccgcc gcttccacct gctggcgagc
      481 ggtcacggcg cggcgcggct gtcgacggcg ttcgattcga ccacgctgta cggcgaagac
      541 ccggccgagc ggcccgacat ctacggccgc atcggcaatt cgggcgtgtc catcgccacg
      601 ctcgacgaca tgaagaagct gtactccggc ttcgacctgt gtgcgccgac gacctcggtg
      661 tcgatgacca tcaacgggcc cgcgccgatg atcctcgcga tgttcatgaa caccgcgatc
      721 gaccagcgcg tcgagcggca cctgcgcgag accgggcagt gggacgcggc gcagcgcagg
      781 atcgacgaac tgctcaagga ccggccgcgg ccggcgtatc gcggcgacct gccgccgacg
      841 cacgacggtg cgggactcgg cctgctcggc gtgaccggcg accagctcgt cgatgccgag
      901 acctatgcgc gcatcaaggc ggcgacgctg cggcaggtgc gcggcacggt gcaggcggac
      961 atcctcaagg aagaccaggc gcagaacacg tgcattttct cgaccgaatt cgcactgcgc
     1021 atgatgggcg acgtgcagca gtacttcacc gaccacgagg tgcgcaactt ctactcggtg
     1081 tctatttccg gctaccacat cgccgaagca ggcgcgaacc cggtgtcgca gctcgcgttc
     1141 acgctcgcca acggcttcac gatcgtcgag tactacctcg cgcgcggcat gcgcatcgac
     1201 gatttcgcgc cgaacctgtc gttcttcttc tcgaacggca tggaccccga gtacgcggtg
     1261 atcggccgcg tggcgcggcg catctgggcg cgcgcgatgc gtgacctcta ccgcgccggg
     1321 ccgcgctcgc agatgctgaa gtaccacatc cagacgtccg gccgcagcct gcacgcccgc
     1381 gagatccagt tcaacgacat ccgcacgacg ctgcaggcgc tgtacgcgct gttcgacaac
     1441 tgcaacagcc tgcacaccaa cgcgtacgac gaggccatca cgacgccgac cgaggaatcg
     1501 gtgcggcgcg cggtcgcgat ccagctgatc atcagcaagg a
//
LOCUS       DBNHBY010000451         2406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1381682, whole genome shotgun sequence.
ACCESSION   DBNHBY010000451 DBNHBY010000000
VERSION     DBNHBY010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1381682"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1502
                     /locus_tag="AC16HB_11210"
                     /pseudo
     CDS             206..1502
                     /locus_tag="AC16HB_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            1508..2320
                     /locus_tag="AC16HB_11215"
     CDS             1508..2320
                     /locus_tag="AC16HB_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR5607404.1"
                     /translation="MSTAPAWLGIGSIRHARLRPTRNRFAYRAWFLRLPRRARERQPW
                     LFRGLARNRRGVFALNDADHGDGRPLLAWIDDLLQREGIADATGEIWLHTLPRVFGYV
                     FNPVSFWFCERADGALRAIVCEVNNTFGERHCYLLAHAEGRSIRWGEELSARKVFHVS
                     PFCRVEGRYRFRFLLSNRDAPPRFIARIDHDDASGPLLQTSIEGVLAPLTGARLARTL
                     ITHPAFTVGVIARIHWQALRLWLKRVAFNSRPRPPAYGVTRSNTDSPSASAP"
BASE COUNT          326 a          880 c          802 g          398 t
ORIGIN      
        1 gtcgaggcgg aaaacttcga atcgatccac aaccactcgg cctatgcgct gatcgagcac
       61 gacaagcgca aggactcgcc agacgcctag ccgccgctga gttcccggcc tggcgtccag
      121 gccggtcggc cgggtcggtc gtatacagca gggagttatc ggtcgcgccg cggccgggtt
      181 cccccagcct gggggtctgc tgcgcatgaa gatcgcgatc atcggggccg gcatcgccgg
      241 gctcggctgc gcgcatgcgc tggcgcgcga cgtcggcccg gagcaccgcg tcacgctgtt
      301 cgaatccgcc gcgcgcctcg gcggccacac caacacggtc gacgtcacgc tcgacggcgt
      361 tgcgcacccg gtcgacaccg gcttcctcgt cttcaatgaa cgcacgtatc cgcgcctgat
      421 cgcgctgttc cgggaactcg aggtcccgat cgccgcgagc gacatgtcgt tctcggtcag
      481 cgcggccgag cgcaatgggc agcgcctcga atgggccgga acgaacctgt caaccgtatt
      541 cgcgcagcga cgcaacctcg cgtcgccgca cttcctgcgc atgctggcgg acctgctgcg
      601 cttcaaccgc cacgccaccc gcgtggccga aggacgcgag gacgcggacg gcgtatcgct
      661 cggcgacttc ctcgaccgcc atcgctattc ggaggcattc cggcgctggt atctgctgcc
      721 gatggcggcg gcgatctggt cgtgcccgac cgcgacgatg ctcgcgtatc cactcgcgac
      781 cttcgtgcgc ttctgccaca accacggcct gctgcaggtc agcgaccggc cgcgctggtt
      841 cacggtgcgt ggcggtgcgc gccagtacgt cgagcgcatc gccgcgcgcc tgcccgacgt
      901 tcggcgcggc attgcggtgg cgcgcgtcga gcatgatccg cgcggcggac tcgccgtcgt
      961 cgtgccgcgc gggcgcgagc gcttcgacca cgtcgtgttc gcgtgccaca gcgatcagtc
     1021 attgcgcatc ttcgccgacg cggaccgcga gcaacgcgcg ctgcttggcg cggtgtgcta
     1081 ccagccgaac cgcgccgtgc tgcacacgga cgagcggctg atgccgcgcg cgcagcgcgt
     1141 gggcggcgtg gaactatctc agcgatgaca ccgccgacgg ggcaagcggt gtgtcggtca
     1201 cgtactggct caatcgcctg cagccgctgc cgttcgcgcg cccggtgttc gtcagcctca
     1261 atccgctgac cgagccgcgc gccgagtccg tgatcgcgga attcgactac gcgcatccga
     1321 tcttcgacga cggcgcgatc gccgcgcagc gtgcgcttgc cgacatgcag gggcggcgcg
     1381 gcgtctggtt cgccggtgca tggaccgggc acggcttcca tgaggacggg ctgaggtccg
     1441 gcctcgcggt cgcggccgct ctgaccgcgc aggtgccagc atcgctgccg ctggcggcgt
     1501 gatcgcgatg agcaccgcgc ccgcctggct cggcatcggt tcgattcgcc acgcgcgact
     1561 gcgtccgacg cgcaatcgct tcgcctatcg ggcgtggttc ctgcggctgc cgcggcgcgc
     1621 gcgggagcgg cagccgtggt tgttccgcgg gctcgcgcgc aatcgccgcg gcgtattcgc
     1681 gctcaatgat gcggaccacg gcgacggccg gccgctgctc gcctggatcg acgaccttct
     1741 gcagcgcgaa ggcatcgcgg atgccaccgg cgagatctgg ctgcacacgc tgccgcgcgt
     1801 gttcggctac gtgttcaacc ccgtcagctt ctggttctgc gagcgcgccg acggcgcgct
     1861 gcgcgcgatc gtctgcgagg tcaacaacac gttcggcgaa cggcactgct atctgctcgc
     1921 gcacgcggaa ggacgcagca tccgctgggg cgaggaactg tcggcgcgca aggtcttcca
     1981 cgtttcgccg ttctgccgcg tcgagggccg ctaccgcttt cgcttcctgc tgtcgaaccg
     2041 cgatgcaccg ccgcgcttca tcgcgcgcat cgaccacgac gatgcctccg ggccgctgct
     2101 gcagacctcg atcgagggcg tactcgcgcc gctgaccggc gcacggctcg cgcgcacgct
     2161 gatcacgcat ccggcgttca ccgttggcgt gatcgcgcgc attcattggc aggcgctgcg
     2221 cctgtggctc aagcgcgtcg cattcaactc ccggccgcgg ccgcccgcgt acggcgtcac
     2281 gcgctccaat accgattcac cctcagcctc tgcgccatga acgagttcgc ccttaccccc
     2341 acacctgccg cctcgattgc gctgccactg cgccagcgct ggctgctgca tctcctggca
     2401 cgcctc
//
LOCUS       DBNHBY010000452         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_95463, whole genome shotgun sequence.
ACCESSION   DBNHBY010000452 DBNHBY010000000
VERSION     DBNHBY010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_95463"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="AC16HB_11220"
     CDS             <1..419
                     /locus_tag="AC16HB_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5607405.1"
                     /translation="PIGIMQACMDVVTPYIHDRKQFGTSIGEFQLIQGKVADMYTTLA
                     ACRAYCYTVGKNLDTLGREHVRQVRKDCAGVILYTAEKATWMAGEAIQILGGNGYINE
                     YPTGRLWRDAKLYEIGAGTSEIRRMLIGREYFAETM"
     gene            426..1352
                     /locus_tag="AC16HB_11225"
     CDS             426..1352
                     /locus_tag="AC16HB_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5607406.1"
                     /translation="MPLHTHTPVLFAPPSYSRAARPLALKMDALQPCGSFKLRGIGLA
                     AERAVARGAREIVCPSGGNAGFAAAYAGAQLKVPTTIVVPQTTLPSVRARVAAIGASV
                     IVHGAVWDESNEHALLLARGQNAAYLHAFDDPDIWDGHASLIDECVADGVEFDAVACC
                     VGGGGLLAGIVQGLRRNGRRDVPVIAVETEGAASYAAALARGEPVTLPAITSIATSLG
                     AKRVCARAVELAREHEIVSLTVTDAQAVAACLDFADAFRVLVEPACGATLAALDAHPE
                     RFARFGRVLVEICGGIGVSLGKLAEWRAQFEL"
BASE COUNT          230 a          508 c          537 g          235 t
ORIGIN      
        1 ggccgatcgg catcatgcag gcctgcatgg atgtcgtcac gccctacatc cacgaccgca
       61 agcagttcgg cacctcgatc ggcgagttcc agctgatcca gggcaaggtc gccgacatgt
      121 acaccacgct cgccgcgtgc cgtgcgtact gctacacggt cggcaagaac ctcgacacgc
      181 tcggccgcga gcacgtgcgc caggtgcgca aggactgcgc cggcgtgatc ctctacacgg
      241 cggagaaggc gacctggatg gccggcgagg cgattcagat cctcggcggc aacggctaca
      301 tcaacgagta cccgaccgga cggctgtggc gcgatgccaa gctgtacgaa atcggcgcgg
      361 ggacttcgga gattcgcaga atgctcattg ggcgcgagta tttcgccgag acgatgtagc
      421 tggtcgtgcc gctgcacacg cacaccccgg tcctgttcgc gccaccttcg tacagccgcg
      481 cagcgcgtcc gcttgcgctc aagatggacg cgctgcagcc gtgcgggtcg ttcaagctgc
      541 gcggcatcgg gctggcggcg gagcgcgcgg tcgctcgcgg cgcgcgcgaa atcgtctgtc
      601 cgtcgggggg taatgccggc ttcgccgccg cttacgccgg tgcgcagctg aaggtgccga
      661 ccacgattgt cgtgccgcag acaaccctgc ccagcgtgcg cgcgcgcgtc gccgccatcg
      721 gtgcgagcgt gatcgtgcac ggcgcggtgt gggacgaatc gaacgaacac gcgctgttgc
      781 tcgcgcgtgg gcaaaacgcg gcgtacctgc acgcgttcga cgacccggac atctgggatg
      841 gccacgcgag tctgatcgac gagtgcgtgg ccgacggcgt ggagttcgac gcggtcgcct
      901 gctgcgtcgg cggcggcgga ctgctggccg gcatcgtgca gggactgcgg cgcaacgggc
      961 ggcgcgacgt gccggtgatt gcggtggaga ccgaaggcgc ggcgtcgtac gcggccgcgc
     1021 tcgcgcgcgg ggagcccgtc acgctgccgg cgatcacgtc gatcgcgacc tcgctcggcg
     1081 ccaaacgcgt ctgcgcgcgc gccgtcgagc tggcgcgcga gcacgaaatc gtttcactca
     1141 ccgtgaccga cgcgcaggca gtcgccgcgt gtctggactt cgccgacgcc ttccgcgtgc
     1201 tggtggagcc ggcgtgcggc gccacgctcg ccgcgctcga tgcgcatccg gagcgcttcg
     1261 ctcggttcgg ccgcgtgctg gtcgagatct gcggcggcat cggcgtgtcg ctcggcaagc
     1321 tcgcagaatg gcgggcgcag ttcgaactgt aatagctaac gaaaggagcc gcgatgcacc
     1381 cgtggctgac cgaaatggaa cggctgcgcg cccgcactgc gcagcccggg ctcctcgatc
     1441 cgaagctcgc cgccggcctg agcggcctcg atttcctgct gcgaatcgtg cgcggcgaga
     1501 ttcccgatcc
//
LOCUS       DBNHBY010000453         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1382067, whole genome shotgun sequence.
ACCESSION   DBNHBY010000453 DBNHBY010000000
VERSION     DBNHBY010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1382067"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /gene="tldD"
                     /locus_tag="AC16HB_11230"
     CDS             complement(<1..1468)
                     /gene="tldD"
                     /locus_tag="AC16HB_11230"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR5607407.1"
                     /translation="MKPSDPAFERLAIARGLLLEPYELDENTLQRALAIVFEHDADFA
                     DIYFQYTRNESFALEEGIVKSGSFDIQQGVGVRAVVGDKTAFAYSDDLSQDALVEAAR
                     TVRSIARAGGGAAKVATKFATRKATPLYAAIDPVASMNAVQKVQLLERIERMARARDA
                     RVKQVMASISGEYDVVLVARADRKAAAVLAADVRPLARVSVTVIVEDGGRREQGFSGG
                     GGRVDLAYFNDARLAEYVQVAVDMALTNLRAQPAPAGVMTVVLGAGWPGILLHEAIGH
                     GLEGDFNRKGSSAFSGRIGQRVASKGVTVVDDGTIPERRGSLTVDDEGNPTSRTVLIE
                     DGVLRGYIQDSLNARLMKTAPTGNGRRESFAFLPMPRMTNTYMLGGGHAPEEIIRSVK
                     KGLYATNFGGGQVDITNGKFVFSASEAYLIEDGHVTAPLKNCTLIGNGPDVLTRVSMV
                     GNDLKLDEGVGTCGKDGQSVPVGVGIPTLKVDRITVGGT"
     gene            complement(1504..>1896)
                     /locus_tag="AC16HB_11235"
     CDS             complement(1504..>1896)
                     /locus_tag="AC16HB_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5607408.1"
                     /translation="TFTFQPSGGPQVKAGLSVCYDLRFPELYRALEQPDLILVPSAFT
                     ATTGKAHWETLLRARAIENLCYVLAPAQGGKHENGRATYGHSMLIDPWGEVVASRDEG
                     PGVVIGDVDLARIADVRASLPALAHRVL"
BASE COUNT          289 a          657 c          629 g          321 t
ORIGIN      
        1 ccgttccgcc gacggtgatg cggtccactt tgagcgtcgg aatgccgacg ccgactggaa
       61 ccgactgccc gtctttgccg caggttccga cgccttcgtc gagcttgagg tcgttgccca
      121 ccatggaaac gcgcgtaagc acgtcgggac cgttgccgat gagtgtgcaa ttcttgagcg
      181 gcgcggtgac gtggccgtcc tcgatcaggt acgcctcgga ggcggagaag acaaacttgc
      241 cattggtgat gtcgacctgc ccgccgccga agttggtcgc atacaacccc ttcttgaccg
      301 agcggatgat ttcctctggc gcgtgcccgc cgccgagcat gtatgtgttg gtcatgcgcg
      361 gcatcggcaa gaatgcgaag gattcacgcc ggccgtttcc ggtcggcgcg gtcttcatca
      421 accgcgcatt cagcgagtcc tggatgtagc cgcgcagcac gccgtcctcg atcaacaccg
      481 tgcgcgaggt cgggttgccc tcgtcatcga ccgtgagcga tccacgccgc tcggggatcg
      541 tgccgtcgtc gacgacggtg acgcccttcg acgcgacgcg ctgcccgatc cgcccggaaa
      601 acgcgctcga ccccttgcgg ttgaaatcgc cttcgaggcc gtggccgatc gcctcgtgca
      661 gcaggatgcc gggccagccg gcaccgagca cgaccgtcat cacgccggcc ggtgcgggct
      721 gcgcgcgcag gttcgtgagc gccatgtcca cggccacctg cacgtactcg gccagccgcg
      781 cgtcgttgaa gtaggcgagg tcgacgcgcc cgccgccgcc cgagaagccc tgctcgcgcc
      841 ggccgccgtc ctcgacgatc accgtcaccg acacccgcgc cagcggccgc acgtcggccg
      901 ccagcaccgc ggccgccttg cggtcggcgc gtgccaccag cacgacgtcg tactcgccgc
      961 tgatgctcgc catcacctgc ttgacgcgcg cgtcgcgggc gcgggccatt cgttcgatgc
     1021 gctcgagcag ttgcaccttc tgcaccgcgt tcatcgacgc gaccggatcg atcgccgcgt
     1081 acagcggcgt ggccttgcgc gtggcgaatt tggtcgcgac cttcgccgcg ccgccgccgg
     1141 cgcgtgcgat cgagcgcacg gtgcgcgcgg cctccaccag cgcgtcctgc gacaggtcgt
     1201 ccgagtacgc gaacgcggtc ttgtcgccga cgacggcgcg cacgcccacg ccctgctgga
     1261 tgtcgaagct gccgctcttg acgatgcctt cctcgagcgc gaacgactcg ttgcgcgtgt
     1321 actggaagta gatgtcggcg aaatcggcgt cgtgctcgaa cacgatcgcc agggcacgct
     1381 gcagcgtgtt ctcgtcgagc tcgtatggtt cgagcagcag cccgcgcgca atggcgaggc
     1441 gttcgaaggc aggatcggag ggtttcatcg gaccgaggag tcgatatgca gttggagcgc
     1501 gggtcacaac acccgatgcg caagtgccgg caggctcgca cgcacgtcgg cgatgcgcgc
     1561 gaggtcgacg tcgccgatca ccacgccagg gccttcgtcg cggctggcga cgacctcgcc
     1621 ccacggatcg atcagcatgc tgtggccgta ggtggcgcgg ccgttctcgt gtttgccgcc
     1681 ttgcgcgggc gcgagcacat agcacaggtt ttcgatcgcg cgcgcgcgca gcagcgtttc
     1741 ccaatgcgcc ttgcctgtcg tcgcggtgaa cgccgacggc accaggatca gatcgggctg
     1801 ttccagcgcg cgatacagct ctggaaagcg caggtcgtag cagaccgaca atccggcctt
     1861 cacctgcggg ccgcccgacg gctggaacgt gaacgt
//
LOCUS       DBNHBY010000454         1798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1178450, whole genome shotgun sequence.
ACCESSION   DBNHBY010000454 DBNHBY010000000
VERSION     DBNHBY010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1178450"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..572)
                     /locus_tag="AC16HB_11240"
     CDS             complement(201..572)
                     /locus_tag="AC16HB_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607409.1"
                     /translation="MSTIAKLQLALDRQQNSAAAVVTLEHRELFPLHAGHYGGHHRPV
                     VEVECQRELFHARIMTGRRARLAGQCFADHQRFGSSRELISGLIRSIDRRIIRGVIVG
                     RIECSLQGACWSASASPVTST"
     gene            833..1243
                     /locus_tag="AC16HB_11245"
     CDS             833..1243
                     /locus_tag="AC16HB_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607410.1"
                     /translation="MSVAHEIRRLGFRRWYEGQLIRSHVYLAAAFFGLIILLSGIEMF
                     DLAQRRPAYYLANLVIAAAAGTGMLIGWKRFNVLLARAEQFAAAAECPGCKAWGKFSV
                     LAAESESDDDPPEAGRPHWVHVRCAKCGSDWKLG"
     gene            1276..1788
                     /locus_tag="AC16HB_11250"
     CDS             1276..1788
                     /locus_tag="AC16HB_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HIR5607411.1"
                     /translation="MKNFETLLEALIFRSRWLLAPFFIGLLIAIAALLLKFAKELLTM
                     ALGLFSLTGSDLIVSILTLVDTALIASLLLIIAFSGYENFVSKIATGEHEDRPAWMGK
                     VGFADLKIKLMGAIVAISAVELLKGFINVDQYTQQQLAWKVGIHLTFVVSGVMFAITD
                     RISEGKGESH"
BASE COUNT          326 a          531 c          621 g          320 t
ORIGIN      
        1 cggcggcgga tcgcgcttcg cgcgtgtgaa acgacgcgta gtgtgacccg tactcggccg
       61 gagacatctt tcgcgccaaa cgcgagcgaa ggtgcagccg gggcggatcc agaccgtcgt
      121 accgaccgac atcgcggtga tgtccggcgc agcgatgcaa ccgccgaaga gccggcggca
      181 ctcgacgcat gcggcaccgc tcaggtggag gtgaccggcg aagcactcgc actccagcac
      241 gcaccctgca gggaacattc gattcggccg acgatgacac cgcggatgat gcgacggtcg
      301 atcgagcgaa ttagcccgct gatcagctcg cgacttgaac cgaaccgctg gtgatccgcg
      361 aagcactggc cggcgaggcg ggcacgccgc ccggtcataa tccgcgcatg gaagagctca
      421 cgctggcatt cgacctcgac gaccggccga tggtggccgc cgtagtggcc ggcatgaagc
      481 gggaacagtt cgcggtgctc gagagtgacg acggcggccg cggaattctg ctggcgatcg
      541 agcgcaagct gaagcttggc aatcgtcgac atgggggagc cgggcaagtc gggcctggac
      601 atggcgtcgt aagtggcggc ccgtaccgac gccggcatcg tctagttcgc gatcggcatc
      661 tcgtgggagc ggctgcgtcg agaaagcgcg cgcgcgctcg atgggttacg cccaccggcg
      721 cgggccgagt gccgcagatt cagcgagttg cctgcgcaca tggccgacgg ggtcaaaaat
      781 cacggtacgc tggtttgcag ggccgacggc ggcgcgcgtt acatctgccc cgatgagcgt
      841 cgcgcacgaa atccgacggc tcggctttcg gcgctggtac gagggccaac ttatccgcag
      901 ccacgtatat ctcgcggcgg cgttcttcgg gctgatcatt ctgctgtctg gaatcgagat
      961 gttcgatctg gcgcagcgca ggccagcgta ctacctggcc aatcttgtca tcgcagcggc
     1021 cgcgggcaca ggcatgctga tcggctggaa gcgcttcaac gtgctgctgg cacgcgcaga
     1081 acagttcgcc gccgcagccg agtgcccggg ctgcaaggca tggggcaagt tctcggtcct
     1141 ggcggccgag agcgaaagcg atgacgaccc gccggaggct gggcgtccgc actgggtgca
     1201 cgtgcgctgt gccaagtgtg gctccgactg gaagctgggc tgacgcgagc gatcggggcg
     1261 ctgaggagac cgaacatgaa gaatttcgaa acgctgctgg aggcgttgat ctttcgcagc
     1321 cgctggttgc tcgcgccgtt ctttatcggg ttgctcatcg cgatcgccgc gctgttgctg
     1381 aagtttgcca aggagttgct gacgatggca ctgggcctgt tcagcttgac cggatcggac
     1441 ctgatcgtgt cgatcctcac gttggtcgat accgcgctga tcgcctcgtt gctgctgatc
     1501 atcgcgttca gcggctacga gaactttgtg tcgaagatcg ctaccggtga gcacgaggac
     1561 cgaccggcct ggatgggcaa ggtcggcttc gcggacctga agatcaagct gatgggcgcg
     1621 atcgtcgcaa tctcggcggt cgaactgctg aagggattca tcaatgtcga ccaatacacg
     1681 cagcaacagc tggcgtggaa ggtcggcatc cacctgacct tcgtcgtttc cggcgtgatg
     1741 ttcgcgatta cggatcggat cagcgagggg aaaggcgaga gccactgagt gttgctct
//
LOCUS       DBNHBY010000455         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_569669, whole genome shotgun sequence.
ACCESSION   DBNHBY010000455 DBNHBY010000000
VERSION     DBNHBY010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_569669"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16HB_11255"
     CDS             complement(<1..233)
                     /locus_tag="AC16HB_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5607412.1"
                     /translation="MTRGIDVRQLRYFIAVAQERNFRRAAERLHITQPPLSRQIGALE
                     AALGTRLLARDTRRVELTAAGEVALREFERAVK"
     gene            420..1721
                     /locus_tag="AC16HB_11260"
     CDS             420..1721
                     /locus_tag="AC16HB_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5607413.1"
                     /translation="MAALLAACGGSGTAPADGPAIAQFTADRSAYFVGEKARLTVNYR
                     GGAGRIEPGIGAVANGAVVETPVLDRGTTYRLIVEGGGKSVAQSLPLPVDFRDRFVAL
                     DVPFVSSEHAAVTTGDGAVLILGGSRGLNTLSEAVDRFDPATRTFTRIGSMRTGRSGH
                     SATRLPDGRILVFGGASSLQIGNVADLIDERTGAVSHGGDLVQPRSWHASTRLADGRV
                     LVTGGYGRNSAEIWDPATNTWRLVAARMSQMRWHHTATLLADGRVLIAGGQSDGTAYQ
                     FAEWFDPRTETFVPVPSTGIEMRRLHAAHRLGDGGVLIVGGETFDANGQIVRLASALR
                     FDAVANRFVAAAPLATARTLMRSIALPDDRVLLFGGETPQARPSASSEAYSAAQGGRA
                     LAQMMGERMLHSVSRLPDGRILVVGGEGAGGAYQAQVLLYE"
     gene            1815..>2403
                     /locus_tag="AC16HB_11265"
     CDS             1815..>2403
                     /locus_tag="AC16HB_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607414.1"
                     /translation="MSEPAPPVASAAPHSAWVPMRRPVFRMLWLTWLASNVGMWMNDV
                     AAAWLMTSLTTSPIMVALVQSASSAPVFLLGVPSGALADILDRRHYYLATQCWVAFNA
                     IVLFIVVFAGWLTAPLLLLLTFANGIGMAMRWPVYSAIVPDLVPRDELHAAIGLNGIA
                     MNASRVIGPVAAGALLASLGGAWVFALNAVLAVGAA"
BASE COUNT          321 a          836 c          844 g          402 t
ORIGIN      
        1 tgcttgaccg cacgctcgaa ctcgcgcagc gcgacttcac ccgccgctgt cagttcgacg
       61 cgtcgcgtgt cgcgcgccag caggcgggtc ccgagcgcgg cttcgagcgc gccgatctgg
      121 cgtgacagcg gcggctgcgt gatgtgcagc cgctccgcgg cgcggcggaa gttgcgctcc
      181 tgcgccacgg cgatgaagta acgcagctgg cggacgtcga ttccccgagt catacccaaa
      241 aggtattgcc aaaaacgcaa ttctgccaag cggcgggcct gcctacagtc gcccgctttg
      301 cccgcgcagc gcctgcgacg gccagcgcaa tcttccttct cctggagccc aacatgtctg
      361 atttgctctg caaacacact ttcgccatgc gtctcgcagc ggcatccctg atcgtagcgg
      421 tggccgcgct gctggccgct tgcggcggtt cgggcacggc gcctgcggat ggacccgcga
      481 tcgcgcaatt caccgcggac cgcagcgcgt atttcgtcgg cgagaaggcg cggctgacgg
      541 tcaactaccg cggcggcgca ggccggatcg aacccggcat cggcgcggta gccaatggcg
      601 ccgtcgtcga aacgcccgtg ctcgaccgcg gcacgaccta tcgactgatc gtcgagggcg
      661 gcgggaaatc agtcgcgcaa tcgcttcctt tgccggtgga ctttcgcgat cgtttcgtgg
      721 cgctcgacgt tccgttcgtc agttccgaac acgcggcggt gaccaccggc gacggcgcgg
      781 tcctgatcct cggcggatcg cgcggtctga acacgctatc ggaggccgtc gaccggttcg
      841 atcccgccac gcgcaccttc acgcgcattg gttcgatgcg caccggccgc agcggccaca
      901 gtgccacccg cttgccggac ggacgcattc tggtgttcgg cggcgcgtcg agcctgcaga
      961 tcggcaacgt cgccgacctg atcgacgagc gcaccggcgc cgtctcccac ggcggcgatc
     1021 tcgtgcaacc gcgcagttgg cacgcgtcga cgcggctcgc cgacggacgc gtgctcgtga
     1081 ccggcggcta tggccgcaac agtgccgaga tctgggatcc ggccacgaac acgtggcggc
     1141 tcgtcgccgc gcgcatgtcg caaatgcgct ggcatcacac cgcgacgctg cttgccgacg
     1201 gccgggtact gatcgccggc ggccagtccg acggcacggc ctaccagttc gccgagtggt
     1261 tcgacccgcg taccgagact ttcgtaccgg tcccgtcgac cggcatcgag atgcgccgcc
     1321 tgcacgccgc gcatcggctc ggcgacggtg gcgtgctgat cgtcggcggc gagaccttcg
     1381 acgcgaacgg ccagatcgta cggctggcga gtgcactgcg cttcgatgcc gtcgccaacc
     1441 gcttcgtcgc cgccgcgccg ctggccactg cgcgcactct gatgcggtcg atcgcgttgc
     1501 ccgacgaccg cgtgctgctg ttcggcggcg agacgccgca ggccaggccg agcgctagca
     1561 gcgaggcgta ctccgccgcg caaggcggcc gcgcgctggc gcagatgatg ggcgagcgca
     1621 tgctgcacag cgtgagccgg ttgccggacg ggcgcatcct cgtggtcggc ggcgagggcg
     1681 cgggcggcgc ctaccaggcg caggtcctgt tgtacgagtg atcgagcgct gacgaggcgc
     1741 gggccggcgg cccgccgcgg ggacgcacgg cctgcgagaa tcgcgaccgt cgtcgatttc
     1801 ctccgttggt cgcgatgagc gaacccgcgc ctcccgtcgc atcggctgcg ccgcactccg
     1861 cctgggtgcc gatgcggcgc ccggtgttcc gcatgctgtg gctgacgtgg ctggcgtcga
     1921 acgtcggcat gtggatgaac gacgtggcgg cggcgtggct gatgacgtcc ctcaccacgt
     1981 cgccgatcat ggtggcgctg gtgcaatcgg cgtcgtcggc gccggtgttc ctgctcggcg
     2041 tgccgagcgg cgcgctggcc gacatcctcg accgccgcca ctattacctc gccacgcagt
     2101 gctgggtcgc gttcaacgcg atcgttctgt tcatcgtcgt gttcgccggc tggctgacgg
     2161 cgccgctgct gcttctgctc acgttcgcca acggcatcgg tatggcgatg cgctggccgg
     2221 tgtattcggc gatcgtgccg gacctcgtgc cgcgcgacga actgcacgcc gcgattggcc
     2281 tgaacggcat cgcgatgaac gcctcgcgcg tgatcgggcc ggtcgcggcg ggcgcgctgc
     2341 tggcgagcct gggcggcgcg tgggtgttcg cgttgaacgc ggtgctcgcg gtcggtgcgg
     2401 cgt
//
LOCUS       DBNHBY010000456         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_28409, whole genome shotgun sequence.
ACCESSION   DBNHBY010000456 DBNHBY010000000
VERSION     DBNHBY010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_28409"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC16HB_11270"
     CDS             <1..379
                     /locus_tag="AC16HB_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5607415.1"
                     /translation="ELLAAVHVPAPRPTCASGYRKARVRGAMDFPLAGVVVAMCQRDG
                     LVAELRVALTGTNARPFVLDGTQPLIGRPVDEPVLAALGKLIAKQVSPMRSTATPSNY
                     RRQVATVLAQRLLRELADGLPRP"
     gene            456..923
                     /locus_tag="AC16HB_11275"
     CDS             456..923
                     /locus_tag="AC16HB_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5607416.1"
                     /translation="MNFTRQVNRALDEEHRANLDLLARVEQVFGKSGGRDAALAATFG
                     RQIELELSRHFGFEERDLFPRITDAGAGDLAALLLEEHETIRRVAAELLPLARAAATL
                     DDGGWAAFRRSALEMAERLRAHIDKETAALLPAIEDALDEDTDRELAFAYAAS"
     gene            936..>1755
                     /locus_tag="AC16HB_11280"
     CDS             936..>1755
                     /locus_tag="AC16HB_11280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607417.1"
                     /translation="MQTANIRPLTRDDLGAVVAIDTALEGRPRRDYVERRLAAALREP
                     KLHAQFAATDGHGLAGYILARVLEGEFGRTTPGLRLELVGVRADVRGRGVGRRLFDAL
                     AQWGRRHGVEDIRTVADWRDAPMLRYFDAMGFELAPHQIIDCAVDGGAYSAARDETAT
                     PAAENRELDFSAPAGNDFEKLARDTADVRSMTAADLTEIERIDRRIIGRDRSGYIEAR
                     LGEAMQDSAIRVSLTARSDGAIAGYLMARADLGDFGRTEPVAVIDTLGVDPGFAN"
BASE COUNT          265 a          619 c          621 g          250 t
ORIGIN      
        1 cgaattgctg gctgcggttc acgtgccggc gccgcgcccg acctgcgcct ccggctatcg
       61 caaggcacgc gtgcgcggcg cgatggactt cccgctcgcc ggcgtggtgg ttgcgatgtg
      121 ccagcgcgac gggctcgtcg ccgagttgcg cgtggcactg acggggacca atgcgcgtcc
      181 gttcgtgctc gacggcaccc agccactgat cggccggccg gtcgacgaac ccgtgctggc
      241 cgcgctcggc aaactgatcg caaagcaggt gagcccgatg cgctcgaccg ccacgccgtc
      301 gaactaccgc cgccaggtgg ccaccgtgct ggcgcagcgg ctgctgcgcg aactggccga
      361 cggcttgccg cggccttgag ttgcatcaag gggcgggcgg tggcctatat ggtattttgg
      421 taccacaata ccgatttagc cgccggaggc gttccatgaa tttcacgcgc caggtcaacc
      481 gcgcactcga cgaggagcac cgcgccaacc tcgacctgct ggcgcgcgtc gagcaggtgt
      541 tcggcaagag cggcggtaga gacgccgcgc tggccgcgac cttcggccgc cagatcgagc
      601 tggaactttc gcgccacttc ggctttgagg agcgcgacct gttcccgcgc atcaccgacg
      661 ccggcgcggg cgatctcgcc gccctgctgc tggaggaaca cgagacgatc cgacgcgtcg
      721 cggccgaact gctgccgctg gcacgcgcgg cggccacgct cgacgacggc ggctgggccg
      781 cgttccggcg cagcgcgctc gaaatggccg agcgtctgcg cgcgcatatc gacaaggaaa
      841 ccgccgcgct gctgcccgcc atcgaagacg cgctcgacga ggacaccgac cgcgagctgg
      901 cattcgccta tgcggcaagc tgacaggggg aaaccatgca gaccgccaat atccggccgc
      961 tgacgcgcga cgatctcggc gcggtggtgg cgatcgacac cgcactggaa ggccggccgc
     1021 gccgcgatta cgtcgagcgc cgcctcgcgg ccgctctgcg cgagccgaag ctgcacgcgc
     1081 agttcgctgc gaccgacggc cacgggctcg caggctacat cctggcgcgt gtgctggagg
     1141 gcgagttcgg tcgcacgacg ccgggcctgc ggctcgaact ggtcggcgtg cgcgccgacg
     1201 tgcgcgggcg cggcgtcggt cggcgcctgt tcgatgcgct cgcgcaatgg gggcgccgac
     1261 acggtgtcga ggacatccgc acggtcgcgg actggcgcga tgcgccgatg ctgcgctact
     1321 tcgacgcgat gggattcgaa ctggccccgc accagatcat cgactgcgcg gtcgacggcg
     1381 gcgcctactc ggcggcgcgc gacgagaccg cgacgcccgc ggccgagaac cgcgagctcg
     1441 atttcagcgc gccggccgga aacgacttcg aaaagctcgc ccgtgacacg gccgacgtgc
     1501 gctcgatgac ggccgccgac ctgaccgaga tcgagcgcat cgaccgtcgc atcatcggcc
     1561 gcgaccgcag cggctacatc gaggcgcgcc tgggcgaggc gatgcaggat tcggcgatcc
     1621 gcgtgtctct gaccgcgcgc agtgacggcg cgattgccgg ctacctgatg gcgcgcgccg
     1681 acctcggcga tttcggccgc accgagccgg tcgccgtgat cgacacgctt ggcgtcgatc
     1741 cgggctttgc caacc
//
LOCUS       DBNHBY010000457         2551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1314991, whole genome shotgun sequence.
ACCESSION   DBNHBY010000457 DBNHBY010000000
VERSION     DBNHBY010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1314991"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="AC16HB_11285"
     CDS             complement(<1..726)
                     /locus_tag="AC16HB_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607418.1"
                     /translation="MTITRRELIQASASAAALTAVPSAFAQAAIEDELTLITPVAKTL
                     TDPTLADFTVYAKEKWNVTLKTSSLAAGTPVAYGRILEWKGRPEADIFWGGESALYDK
                     LAEQKLVAKLDLPKAVLDAIPATIGKPKPIYLKDPKGFWTGTVLEPYGLVYQPKVLQR
                     LGVPEPKDWDDLLHPKLKGHVAQCAPSRSSSSHATYEVILQRDGDEKGWAWLKRLAAN
                     TGIFTARSRDVPSVVARGEFAAGF"
     gene            complement(749..1798)
                     /locus_tag="AC16HB_11290"
     CDS             complement(749..1798)
                     /locus_tag="AC16HB_11290"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5607419.1"
                     /translation="MAKVGVIGGGAFGTAMACVLRRRGHDIELWAREPEVADSINRDR
                     RNAIFLRDVLLPAGIRATNDMHAAVRDAQFVVLAPPAQHMRNVTTALRTGLARGTPVI
                     TCSKGIERGTSALMSQVLADTLPDAPVAVLSGPSFAAEIAIELPTGVTLACGDPVLGE
                     HLETAIGSPRFRVYLADDVIGAQVGGVMKNVLSIACGIATGKRLGNNARATLVARGLA
                     EAVRLGLALGARIETFLGLSGIGDIDLSCNSPQSRNMSLGVALGEGRRLADILAERIT
                     VQEGVHSAEGVVALAARLGVEMPITQAIDQVLNHNADLDRMIAALLTLPFGVETAVVP
                     MLQRSHAAGSVASHR"
     gene            complement(1842..>2551)
                     /locus_tag="AC16HB_11295"
     CDS             complement(1842..>2551)
                     /locus_tag="AC16HB_11295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5607420.1"
                     /translation="PAAAYRALEQLHDAGLRVVPVTGRPAGWCALIARFWPVAAVVGE
                     NGAFYFRYDHAARRMTRRYVRSNAERAHDRARLDAIGAEVLRAVPGTALAADQAYREA
                     DLAIDWCEDVPPLPPREVQRTVDLLHAHGLTVKVSSIHVNAWFGRYDKLTMARRLLAE
                     QFGLDVDAERERVAFVGDSPNDEPMFAFFPNSIAVANIAQFADAIAHKPAYVTRGAEG
                     VGFAELARLLLAARQRR"
BASE COUNT          359 a          921 c          872 g          399 t
ORIGIN      
        1 gaagccggcc gcgaattcgc cgcgcgcgac caccgacggc acgtcccggc tgcgcgcggt
       61 gaagatcccg gtgttcgcgg cgagccgctt gagccacgcc cagcccttct cgtcgccgtc
      121 gcgctgcagg atcacttcgt acgtcgcatg gctgctgctc gagcggctcg gcgcgcactg
      181 cgcgacgtgg cccttgagct tcggatgcag caggtcgtcc cagtccttgg gctccggtac
      241 gccgagccgc tgcagcacct tcggttgata gacgaggccg tacggctcca gcaccgtgcc
      301 ggtccagaat cccttcgggt ccttcagata gatcggcttc ggcttgccga tggtcgcggg
      361 gatcgcatcc agcacggcct tcggcaggtc gagcttcgcg acgagcttct gctcggccag
      421 cttgtcgtac agcgcacttt cgccgcccca gaagatgtcg gcttcgggcc ggcccttcca
      481 ttcgaggatg cgtccatacg ccaccggcgt gcccgccgcc agcgaactcg tcttgagcgt
      541 gacgttccat ttctccttcg cgtagacggt aaagtcggcg agcgtcgggt ccgtcagcgt
      601 cttggcgacc ggcgtgatca gtgtgagttc gtcctcgatc gccgcctgcg cgaacgcgga
      661 cgggaccgcg gtgagggcgg cggcggacgc cgacgcctgg atcagttccc tgcgggtgat
      721 ggtcatgttc tcctcctcgg agtggtcttc atcgatgaga agcaacgctg cctgcggcat
      781 ggctacgctg cagcatgggc acgaccgccg tttcgacgcc gaacggcagc gtcagcagcg
      841 cggcgatcat acggtcgaga tcggcgttgt ggttcagcac ctggtcgatg gcctgcgtga
      901 tcggcatctc gacgccgagc cgcgccgcca gtgcgaccac gccctcggcc gagtgcacgc
      961 cttcctgcac ggtgatccgc tcggccagga tgtcggcgag cctgcggcct tcgcccagcg
     1021 ccacgccgag cgacatgttg cgcgactgcg ggctgttgca cgacaggtcg atgtcgccga
     1081 tgcccgacag cccgagaaac gtctcgatcc gcgcgccgag cgcgagcccc agccgcacgg
     1141 cctcggccag cccgcgcgcg accagcgtgg cacgcgcgtt gttgccgagc cgcttgccgg
     1201 tcgcgattcc gcacgcgatc gacagcacgt tcttcatcac gccgccgacc tgcgctccga
     1261 tcacgtcatc cgccaggtac acgcggaaac gcggactgcc gattgcggtc tccagatgct
     1321 cgcccagcac ggggtccccg caggcgagcg tgacgccggt cggaagctcg atcgcgatct
     1381 cggcggcgaa cgacggcccc gacagcaccg cgaccggcgc gtcggggagc gtgtccgcca
     1441 gcacctgcga catcagcgcg gacgtgccgc gttcgatacc tttcgagcag gtgatgaccg
     1501 gcgtgccgcg agcgagcccc gtgcgcagcg ccgtcgtcac gttgcgcatg tgctgcgcgg
     1561 gcggcgccag cacgacgaac tgcgcgtcgc gcaccgcggc gtgcatgtcg ttggtcgccc
     1621 ggatgcctgc cggcagcagc acgtcgcgca gaaagatcgc gttgcggcgg tcgcggttga
     1681 tcgaatcggc gacctccggc tcgcgcgccc aaagctcgat gtcgtgcccg cggcgccgca
     1741 gcacgcacgc catcgcggtt ccgaacgcgc cgcctccgat gacccccacc ttcgccatgg
     1801 ctcatgctag gcgcggagcg cgagcccgcc gttgatgcgg gtcagcggcg ctgccgcgcg
     1861 gccagcaaca gccgggcgag ttcggcgaag ccgaccccct cggcgccgcg tgtcacgtat
     1921 gccggcttgt gcgcgatggc gtcggcgaac tgcgcgatgt tggccaccgc gatcgagttg
     1981 ggaaagaacg cgaacatcgg ctcgtcgttc ggcgagtcgc cgacgaacgc aacccgctcg
     2041 cgctcggcgt cgacatcaag tccgaactgc tcggcgagca gccggcgcgc catcgtcagc
     2101 ttgtcgtagc ggccgaacca tgcgttgacg tggatcgagc tcactttcac cgtcagcccg
     2161 tgcgcgtgca gcaggtcgac ggtgcgctgc acctcccgcg gcggcagcgg tggcacgtcc
     2221 tcgcaccagt cgatcgcgag gtccgcctcg cggtacgcct ggtccgccgc gagcgcggtg
     2281 ccgggcaccg cgcgcaggac ctcggcgccg atcgcgtcga gccgtgcgcg gtcgtgcgcg
     2341 cgctcggcgt tgctgcgcac gtagcgtcgc gtcatccggc gcgccgcgtg gtcgtagcgg
     2401 aagtagaacg cgccgttttc gccgacgacc gccgcgaccg gccagaagcg cgcgatcagg
     2461 gcgcaccagc ccgccggccg ccccgtcacg ggcactaccc gcaggccggc atcgtgcagc
     2521 tgctccaggg cgcggtacgc cgcggccggc a
//
LOCUS       DBNHBY010000458         9101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_232746, whole genome shotgun sequence.
ACCESSION   DBNHBY010000458 DBNHBY010000000
VERSION     DBNHBY010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_232746"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="AC16HB_11300"
     CDS             <1..104
                     /locus_tag="AC16HB_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5607421.1"
                     /translation="HRQVATGAFGAMMQVELVNDGPVTFWLQVRPRT"
     gene            118..744
                     /locus_tag="AC16HB_11305"
     CDS             118..744
                     /locus_tag="AC16HB_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR5607422.1"
                     /translation="MRLSSKSFADGGRIPGEYAFAVPDPVHHVRLSSNRNPQLAWSDA
                     PAGTKSFVVICHDVDVPSRPDDVNQEGREVPATLPRVDFFHWVLVDLAATTTSIDAGA
                     YSHTVTPRGKSGPAAQHDTRQGFNDYTNWFAGDHDMEGDYYGYDGPCPPWNDSIVHRY
                     VFTVYALDVECAPVQGRFGGADVRRAIEGHVLARASITGTYTLNPRLA"
     gene            737..1381
                     /locus_tag="AC16HB_11310"
     CDS             737..1381
                     /locus_tag="AC16HB_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5607423.1"
                     /translation="MPETTVLLIRHGETAWNAEHRIQGLLDVPLSTTGVWQAARLAER
                     LGAEPIAAIVANDLARAWLTAQPLAQRLGLAPVAEPRVRERDFGSFQGHTVDEIAARW
                     PDEFHAWRARDPAWAVPGGESGHQFIARVLDALQDVALAHAGRMVAVVTHGGVLDVVY
                     RHARNLGWDAPREHQMLNAAINRLSASAPPLRLAVLDWADSAHLDAARDEIAGG"
     gene            1442..2698
                     /locus_tag="AC16HB_11315"
     CDS             1442..2698
                     /locus_tag="AC16HB_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR5607424.1"
                     /translation="MNDAATLALTFASSAAIGLLVGLERERNPRAKAGLRTFALIAVL
                     GAASALVAQATASGWILGAALLAIGISIAGAHLLDPETRADDYGTTTVAAALLVFVLG
                     AINVYGYRLLAVAVGIGMTALLHFKVELEVAAQRLTPTDIRSILQFGAVSAVILPLLP
                     NQPYGPYGVLNPFHIWLMVVLISGVSLAGYVAWRLTLERKGLLLTGLLGGVVSSTATT
                     LVYARHARAGTQSPAAAQVVILLANVAMLVRVVLIIAIIAPAALGGGAVTLLAAALFA
                     LPFALWRMQRGAQEARATDEYSNPTNIATGVIFAAFYASILLVSAWLSDKVGARGVYG
                     LAAISGLTDVDAITLSSLRLLASGAVDLPAAITTICIAVTSNLVLKSIVVFVVGGRRM
                     GRDIAIGFLPAALAMGAGLIVLHALA"
     regulatory      2711..2789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            2830..4089
                     /gene="glyA"
                     /locus_tag="AC16HB_11320"
     CDS             2830..4089
                     /gene="glyA"
                     /locus_tag="AC16HB_11320"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5607425.1"
                     /translation="MYRRTQTVELSDPAVWAAITAENKRQEQHIELIASENYASPAVM
                     AAQGCSMTNKYAEGYPGRRYYGGCEHVDTVETLAIERLKKLFSYPTIELAANVQPHSG
                     AQANEAVFMALLNPGDTIMGMSLAEGGHLTHGMPLNMSGKWFKVVSYGLNAQEAIDYD
                     EMESLAHRHHPKLIIAGASAYSLHIDFKRCAQIARDVNASFMVDMAHYAGLIAADVYP
                     SPVPHADVITSTTHKTLRGPRGGLILCKPALEKQINSAVFPGLQGGPLMHVIAAKAVA
                     FGEALQPEFKTYQQQVLANARVMAETLKQRGLRIVSGGTQSHLMLVDLRAKQITGKDA
                     EAVLGQAHITVNKNAIPKDPEKPMVSSGIRVGSPAMTTRGFREEQAEQTANLIADVLD
                     NPRDPANVARVRDEVEALTRKFPVYGA"
     gene            4148..4615
                     /gene="nrdR"
                     /locus_tag="AC16HB_11325"
     CDS             4148..4615
                     /gene="nrdR"
                     /locus_tag="AC16HB_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5607426.1"
                     /translation="MKCPFCHNADTQVIDSRASDEGATIRRRRKCLNCEKRFTTYERV
                     ELVMPSIIKRGGGRADYDRAKLRSSMLLALRKRPVPREAVDEAIGRIEEKLLGLAARE
                     VKSERLGELVMRELKRLDKVAYIRFASVYRSFEDVDEFAEVVKEVRPPRKRRA"
     gene            4834..5274
                     /locus_tag="AC16HB_11330"
     CDS             4834..5274
                     /locus_tag="AC16HB_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5607427.1"
                     /translation="MANLMVRREPFGSFFDEFFNDFFSRPGMPLARTVELPSVTRARM
                     DVVDKNDRYEVLVDLPGVKKDDIQVTIEGNRVSIAAETKSEQETKEGERLIHSERYAA
                     SYARSFELPVEVTEQGAEAAFENGVLRLQLPKRAAVTSKRLTIR"
     gene            5347..6444
                     /gene="ribD"
                     /locus_tag="AC16HB_11335"
     CDS             5347..6444
                     /gene="ribD"
                     /locus_tag="AC16HB_11335"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5607428.1"
                     /translation="MYAPTDHEFMQRALVLARRALFHATPNPRVGCVIVRDGRVLGEG
                     YTQAPGSNHAEIEALLDARRNGHDLHGATAYVSLEPCAHFGRTPPCATALIEARVARV
                     IAAVEDPNPQVAGQGLEMLRSAGIDVRCGLLQSEAIEMNVGFFARMNRGRPWVRMKIA
                     ASLDGKTALDNGVSQWITSETARADGHAWRARSCAVLTGVGTVQLDNPQLNVRLVETV
                     RQPLKVLVDSRLDVDPAARLFEGAPTLVACARRDVTKEARLRERGIELIDLPNTDGKV
                     DLSALMRELARRGINEVLVEAGFKLNGSLVRAGLVDELLVYLAPLLIGEAQGMVHLPA
                     LSDLAQAQRLVFREVTQIGEDVRLLARWGGH"
     gene            6444..7076
                     /locus_tag="AC16HB_11340"
     CDS             6444..7076
                     /locus_tag="AC16HB_11340"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR5607429.1"
                     /translation="MFSGIVQSTGRIVRADELGFGRRLVIEAPTLGLDDVGIGDSIAI
                     NGACMTVVDKNARTFTIDVSAESLSKTAGLDTRGEVNLEKAMRLGDRIDGHLVSGHVD
                     GTGVVHAIEPRGESVLFEVEAPAQLARYFAYKGSIAVNGVSLTVNRIEDIVRDGKLAG
                     CRASINLIPHTIAVTALRNLKPGDRVNLEVDLIARYVERMLGAADLSQDR"
     gene            7084..8205
                     /locus_tag="AC16HB_11345"
     CDS             7084..8205
                     /locus_tag="AC16HB_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR5607430.1"
                     /translation="MSRLFEPLTLRGLALPNRIMVSPMCQYSALEGNATAWHHAHLGS
                     LALSGAGLVCVEATAVTPEGRITPGCLGLWSDANEAALAPVVATMRAAASGTKLAIQL
                     AHAGRKGSSHAPWDGGQLIAARDGGWQPVGPSALAHAEGELPPRALTREEIVELPRAF
                     AQSAARAARLGFDAIEIHMAHGYLLHEFLSPVANHRSDDFGGSFDNRVRLPLAVFDAV
                     RAAFPAERPVGVRVSATDWLERDATWTLEQTLELARRLQTRGCDWIDVSSGGISPHQK
                     IALGPGYQVPFAQAVKQATGMITIAVGLITAPAQAEAIVAEGRADMVALARAMLLDPR
                     WPWRAAIAVGGKVAAPRQYWRSVPKEASQAFGPIVFGQR"
     gene            8305..>9101
                     /gene="ribB"
                     /locus_tag="AC16HB_11350"
     CDS             8305..>9101
                     /gene="ribB"
                     /locus_tag="AC16HB_11350"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR5607431.1"
                     /translation="MSIASTAEIIDEIRKGAIVVLIDDEDRENEGDLVFAADFVTPEK
                     INFLARFGRGLICMPITAEHAAQLKLAPMAQHNRTVHGTNFTVAIEAAHGVSTGISAA
                     DRAHTIHVASRPNAKPTDIVQPGHVFPLVAQPGGVLMRAGHTEACCDLARLAGLTPAA
                     VLCEIMNDDGTMARLPDLIPFAQKHDMKLGTIADLIHYRAATESLVTRVGSRRVRTLA
                     GEFELVAYRDAPSAGAHLALVFGTPTPDVETLVRVHEPMSALDLIDT"
BASE COUNT         1481 a         3028 c         3185 g         1407 t
ORIGIN      
        1 cgcatcgcca ggtcgccacc ggcgctttcg gtgcgatgat gcaggtcgaa ctggtcaacg
       61 acggtccggt cacgttctgg ctgcaggtca ggccacgcac gtagcagcgc gcccacgatg
      121 agactcagca gcaaatcgtt cgccgacggc ggcaggatcc ccggcgaata tgcgttcgcc
      181 gtgcccgacc cggtgcatca tgtcaggctg tcttcgaacc gcaatccgca actggcgtgg
      241 agtgacgcac cggccggcac gaagtcgttc gtcgtgatct gccacgacgt cgacgttccg
      301 agccggccgg acgatgtcaa ccaggagggg cgcgaggtgc cggccacgct gccgcgcgtc
      361 gacttcttcc actgggtcct ggtcgacctg gcggcgacga cgacctcgat cgacgccggt
      421 gcgtattcgc acactgtgac gccgcgtggc aagagtggcc cggccgctca gcacgacacg
      481 cggcagggct tcaacgatta cacgaactgg ttcgccggcg atcacgacat ggaaggcgac
      541 tactacggct atgacgggcc gtgcccgcca tggaacgatt cgatcgtgca tcgctacgtt
      601 ttcaccgtgt acgcgctcga cgtcgaatgc gcgccggtac aaggtcgttt cggcggcgcc
      661 gacgtgcggc gcgcgatcga agggcacgtg ctcgcccgtg cgagcatcac cggcacttac
      721 acgctgaacc cgcgccttgc ctgaaacgac cgtcctgctg atccgccacg gcgagaccgc
      781 atggaacgcg gagcatcgca tccagggtct gctggacgtt cctctgtcga cgaccggcgt
      841 gtggcaggcc gcgcggctcg ccgagcgcct cggcgcggaa ccgatcgcgg ccatcgtcgc
      901 caacgatctg gcgcgcgcgt ggctgaccgc tcagccgctg gcgcagcggc tcgggctggc
      961 gccggtcgcc gagccgcgcg tgcgcgaacg cgacttcggc tcgttccagg gccacacggt
     1021 cgacgagatc gccgcacgct ggcccgacga gttccacgcg tggcgcgcgc gcgatcccgc
     1081 gtgggccgtg cccggcggcg agtcgggcca tcagttcatc gctcgcgtgc tggacgcatt
     1141 gcaggatgtc gcgctcgcgc acgccggccg catggtcgcg gtcgtcacgc acggcggcgt
     1201 tctcgacgtc gtgtatcgcc acgcgcgcaa tctgggctgg gatgcgccgc gcgagcacca
     1261 gatgctgaat gcggcgatca accggctgtc cgccagtgcg ccgccgctgc ggctggccgt
     1321 cctcgactgg gccgacagcg cgcatctgga cgccgcgcgc gacgagatcg ccggcggctg
     1381 acgcgcaccg gcggttgccg ttgcgcaagt ccgcgcgtgc ggcggatcct agactgtcgc
     1441 catgaacgac gcggccaccc tggcgctgac ctttgcctcc agcgcggcga tcggtctgct
     1501 ggtcggcctc gagcgcgaac gcaacccgcg cgccaaggcg gggttgcgca cctttgcgtt
     1561 gatcgccgtg ctcggcgcgg caagcgcgct ggtcgcacag gcgaccgcga gcggctggat
     1621 cctcggcgcg gcgctgctgg cgatcggtat ctcgattgcg ggcgcgcacc tgctcgaccc
     1681 cgaaacgcgg gcggacgact acggcaccac gaccgtggcg gcggcgctgc tcgtgttcgt
     1741 gcttggcgcg atcaatgtgt atggctaccg gctgctggcg gtcgcggtcg gcatcggcat
     1801 gaccgcgctg ctgcacttca aggtcgaact cgaggtcgcc gcacagcgac tcacgccgac
     1861 cgacatccgc tcgatcctgc agttcggcgc ggtcagcgcg gtgatcctgc cgctgctgcc
     1921 gaaccagccg tacggcccgt acggcgtgct caatccgttc catatctggc tgatggtggt
     1981 cctgatctcc ggcgtcagcc tggcaggcta cgtggcgtgg cggctgacgc tggaacgcaa
     2041 ggggttgctg ctgaccggat tgctcggcgg cgtggtgtcg agcacggcga ccacgctcgt
     2101 gtacgcgcgc cacgcgcgcg ctggcacgca gtcgccggcc gcggcccagg tcgtgatcct
     2161 gctcgccaac gtcgcgatgc tggtgcgggt ggtcctgatc atcgcgatca tcgcgcccgc
     2221 ggcgctcggg ggcggtgcgg tcacgctgct ggcggccgcg ctgttcgcgt tgccgttcgc
     2281 gctgtggcgc atgcagcgcg gcgcgcagga agcgcgcgcg accgacgaat acagcaatcc
     2341 gaccaacatc gcaaccggag tgatcttcgc ggccttctat gcgtcgatcc tgctggtgtc
     2401 ggcatggctg tcggacaagg tcggggcgcg cggcgtctac gggctggcgg cgatctccgg
     2461 gctgaccgac gtcgacgcga tcacgctgtc gtcgctgcgg ctgctcgcca gcggcgcggt
     2521 cgatctgccg gcggcgatca ccacgatctg catcgcggtg acgtcgaacc tcgtgctcaa
     2581 gtcgatcgtc gtgttcgtcg tcggcgggcg caggatgggc cgggatatcg cgatcgggtt
     2641 tctgcccgcg gcgctggcga tgggcgcggg tctgatcgtt ctgcacgcac tggcataatt
     2701 tcgccgccat ccgatctcgc gtgactggcg cagggtgatc acacccggga agcgcgggga
     2761 acacatcgcc gtacgcctgg gcacgatggc aagtggcaaa acaaacactt gtcgaaagcc
     2821 gaggaaacca tgtaccgccg tacccagacc gtcgagttga gcgatcccgc cgtgtgggcc
     2881 gcgatcaccg cggagaacaa gcgccaggaa cagcacatcg agctgatcgc gtcggagaac
     2941 tacgccagcc ccgcggtgat ggccgcgcaa ggctgttcga tgaccaacaa gtacgccgag
     3001 ggctacccgg gcaggcgcta ctacggcggg tgcgagcatg tcgacacggt cgagacgctc
     3061 gccatcgagc ggctgaagaa gctcttttcg tacccgacga tcgagctggc ggccaacgtg
     3121 cagccgcatt ccggagcgca ggcgaacgag gcggtgttca tggcgctgct gaaccccggc
     3181 gacacgatca tgggcatgag ccttgccgag ggcgggcatc tcacgcacgg catgccgctg
     3241 aacatgagcg gcaagtggtt caaggtcgtc agctacggcc tgaacgcgca ggaggcgatc
     3301 gactacgatg agatggagtc gctcgcgcac cgccatcacc cgaagctgat catcgccggc
     3361 gcgtccgcgt attcgctgca catcgacttc aagcgctgcg cgcagatcgc gcgcgacgtc
     3421 aacgccagtt tcatggtcga catggcgcac tatgcggggc tgatcgccgc cgacgtatat
     3481 ccgagcccgg tgccgcatgc ggacgtcatc acgtcgacca cgcacaagac gctgcgcggg
     3541 ccgcgcggcg ggctgatcct gtgcaagcca gcgctggaaa agcagatcaa cagcgcggtg
     3601 ttcccgggcc tgcagggcgg tccgctgatg cacgtgatcg ccgccaaggc ggtcgctttc
     3661 ggcgaggcgc tgcagccgga gttcaagacg taccagcagc aggtgctggc caacgcgcgt
     3721 gtgatggccg agacattgaa gcagcggggc ttgcgcatcg tctcgggcgg cacgcagagc
     3781 cacctgatgc tggtcgacct gcgcgcgaag cagatcaccg gcaaggacgc cgaggccgtg
     3841 ctcggacagg cccacatcac ggtgaacaag aacgcgatcc caaaggatcc ggaaaagccg
     3901 atggtttcca gcggcatccg cgtcggcagc ccggcgatga ccacgcgcgg cttcagggaa
     3961 gagcaggccg agcagaccgc gaatctgatc gccgacgtcc tcgacaaccc gcgcgacccg
     4021 gccaacgtcg cccgcgtgcg cgacgaggtg gaggcgttga cgcggaagtt cccggtttat
     4081 ggcgcatagg cggcgcgagc ctcatagggg agatgccgcg cgccgcctgc gctctgcctg
     4141 aactcgcatg aagtgcccct tctgtcacaa cgccgacacg caggtgatcg actcgcgcgc
     4201 gtcggatgaa ggcgcgacga tccggcgccg gcgcaagtgc ctgaactgcg agaagcgctt
     4261 caccacctac gagcgcgtgg agctggtgat gccttcgatc atcaagcgcg gcggcgggcg
     4321 cgccgattac gaccgcgcca agctgcgctc gtcgatgctg ctggcactgc gcaagcggcc
     4381 ggttccgcgc gaggccgtcg acgaggcgat cggacgcatc gaggagaagc tgctgggcct
     4441 ggccgcgcgc gaggtcaaga gcgagcggct gggcgaactg gtgatgcgcg aactgaagcg
     4501 actcgacaag gtcgcctata tccgtttcgc cagcgtgtac cgcagtttcg aagacgtgga
     4561 cgagtttgcc gaagtcgtca aggaagtccg tcccccgcgc aagcggcgcg cttgaaaccg
     4621 gcgcggcgct gcctgatatc cgccgcgtcg aaggaggccg ccgaatgcgg tagcggggcg
     4681 gtgactcgat gcgcccgcag gccgccggga gaagcggcct ttgccttttc agcgcggccg
     4741 atggcccggg gtcttgaaac cgtggcgccg cgccgcatct caggggtgtg gccgcaagcg
     4801 gccgaggcaa ggaatccgac tgaggagggt gatatggcga atctgatggt acgacgcgaa
     4861 ccgttcggct cgttcttcga tgagttcttc aatgactttt tcagtcgtcc ggggatgccg
     4921 ctggcacgca ccgtcgagtt gccgagcgtc acgcgcgcgc gtatggatgt ggtcgacaag
     4981 aacgaccgct acgaagtgct ggtcgacctc ccgggcgtga agaaggacga catccaggtc
     5041 acgatcgaag gcaatcgcgt gtcgatcgcc gccgagacca agagcgagca ggaaacgaag
     5101 gaaggcgagc ggctgatcca cagcgagcgc tacgccgcca gctacgcgcg cagcttcgaa
     5161 ctgccggtcg aagtgaccga gcaaggcgcg gaggctgcgt tcgagaacgg cgtgctgcgt
     5221 ctgcagttgc ccaagcgcgc ggcagtcacc agcaaacggc tgacgatccg ctgacggccg
     5281 tacgcgcctc tgtcccggcg ggacagaggc cggcggcgac gggcgccgac cgtacaatcg
     5341 gtcgcgatgt atgcgccgac cgaccatgag ttcatgcagc gcgcgctggt actcgcccgg
     5401 cgcgcgcttt ttcacgccac cccgaatccg cgcgtcggct gcgtgatcgt tcgcgacggc
     5461 cgcgtcctgg gcgagggcta cacgcaggcg ccgggatcga accacgccga gatcgaggcg
     5521 ctcctggacg cgcgccgtaa cggccacgac ctgcacggcg ccaccgccta cgtatcgctc
     5581 gaaccttgcg cgcatttcgg ccgcacaccg ccgtgcgcga ccgccctgat cgaggcgcgc
     5641 gtggcgcgcg tgatcgccgc ggtcgaggat ccgaacccgc aggttgccgg tcaggggctg
     5701 gagatgctcc gcagcgccgg catcgacgtg cgctgcgggc tgctgcagag cgaggcgatc
     5761 gagatgaacg tcggcttctt cgcgcggatg aatcgcggcc gcccgtgggt gcggatgaag
     5821 atcgccgcca gcctggacgg caagaccgcg ctcgacaatg gcgtatcgca atggatcacg
     5881 tcggagacag cgcgcgccga cggccacgcg tggcgcgcgc gctcgtgcgc cgtgctgacc
     5941 ggtgtcggga ccgtgcagct cgacaatccg cagttgaacg tgcgtctcgt cgaaaccgtg
     6001 cgccagccgc tgaaggtgct ggtcgacagc cggctcgacg tcgatcccgc ggcgcggctc
     6061 ttcgaaggcg caccgacgct ggtggcttgc gcccgccgcg atgtgacaaa ggaggcgcgg
     6121 ctgcgcgaac gcggcatcga gctgatcgac ctgccgaaca ccgacggcaa ggtcgacctg
     6181 tccgcgctga tgcgcgaact ggcgcgccgc ggcatcaacg aggtgctggt cgaagcgggc
     6241 ttcaaactga atggctcgct ggtgcgcgcg gggctggtcg acgagttgct ggtgtacctg
     6301 gcaccgctgc tgatcgggga ggcgcagggc atggtgcacc tgccggcgct atcggacctg
     6361 gcgcaggcgc aacggctcgt gttccgcgag gtcacgcaga ttggcgaaga cgtgcgcctg
     6421 ctggcgcgct ggggaggaca ttgatgtttt cgggcatcgt gcagtcgacc ggccggatcg
     6481 ttcgcgccga tgaactcgga ttcggccgcc ggctcgtgat cgaggcgccg acgctcgggc
     6541 tggacgacgt gggcatcggc gattcgatcg cgatcaatgg cgcctgcatg acggtcgtcg
     6601 acaagaacgc gcgtacgttc actatcgacg tgtcggccga atcactgtcg aagacggcgg
     6661 ggctcgacac gcgcggggaa gtgaacctcg agaaggcgat gcgcctgggc gatcgcatcg
     6721 acggtcacct cgtgagcggg cacgtcgacg gcaccggtgt cgtgcacgcg atcgagccgc
     6781 gcggcgaaag cgtcctgttc gaggtcgagg caccggcgca actcgcgcgc tatttcgcct
     6841 acaagggttc gatcgccgtc aacggcgtgt cgctgaccgt caaccggatc gaggacatcg
     6901 tgcgcgacgg caagctggcc ggttgccgcg cgtcgatcaa cctgatcccg cacacgatcg
     6961 cggtgacggc actgaggaac ctcaagcccg gcgatcgggt gaacctcgag gtcgacctga
     7021 tcgcgcgcta cgtcgagcgg atgctcggcg cggcggacct atcccaggat cgctgaggcc
     7081 cgcatgtccc gcctgttcga accactgacc ttgcgcgggc tggcgttgcc gaaccgcatc
     7141 atggtgtcgc cgatgtgtca gtactcggcc ctcgaaggca atgcaaccgc gtggcaccac
     7201 gcgcatctgg gctcgctggc gctatccggc gccggcctgg tgtgcgtgga ggccaccgcc
     7261 gtgacgccgg aggggcggat cacgccgggc tgcctcggcc tgtggtccga tgccaacgag
     7321 gccgcgctgg cgccggtcgt ggcgacgatg cgcgcggcgg cgagcggcac gaagcttgcg
     7381 atccagctcg cccatgccgg ccgcaagggg tcgagccacg cgccgtggga cggcggccaa
     7441 ctcatcgctg cccgcgacgg cggctggcag ccggtcgggc catccgccct cgcgcatgcc
     7501 gagggcgaac tgccgccgcg tgcgctgacg cgcgaggaaa tcgtcgagtt gccgcgcgcg
     7561 ttcgcgcagt cggccgcgcg tgcggcgcgg ctcggattcg acgcgatcga gatccacatg
     7621 gcgcacggct acctgctgca tgaattcctg tcgccggtgg cgaaccatcg cagcgacgac
     7681 tttggcggct cgttcgacaa ccgcgtccga ctgccgctgg ccgtgttcga cgccgtgcgc
     7741 gcggcgtttc ccgccgagag accggtcggc gtgcgcgttt cggcaaccga ctggctcgag
     7801 cgcgacgcaa cgtggaccct cgagcaaacg ctggagctcg cacggcggct tcaaacgcgc
     7861 ggctgcgact ggatcgacgt gtcgagcggc ggcatttcgc cgcaccagaa gatcgcactc
     7921 gggcccggct atcaggtgcc gttcgcgcag gccgtcaagc aggccaccgg catgatcacg
     7981 atcgcggtcg ggctcatcac cgcgccggca caggccgagg cgatcgtcgc cgagggtcgc
     8041 gccgacatgg tcgcgctcgc ccgcgcaatg ctgttggatc cgcgctggcc gtggcgcgct
     8101 gcgatcgcgg tcggcggaaa agtcgccgcg ccacgccagt attggcgctc ggtgccgaag
     8161 gaagcgagtc aggcgttcgg accgatcgtg ttcggccagc gttgaccgcg gggcacgcgt
     8221 aagattctgc tcccggtccc gcccgcggcc gcgccgcaag gaatcagcat gctgtccgcc
     8281 gttcccgcct cgacgaaacc tgcgctgtcg atcgcgtcga ccgcggagat catcgacgag
     8341 atccgcaagg gcgcgatcgt cgtgctgatc gacgacgagg atcgcgagaa cgaaggcgat
     8401 ctcgttttcg ccgccgactt cgtcacgccg gagaagatca acttcctcgc gaggttcggc
     8461 cgcgggctga tctgcatgcc gatcactgcc gagcacgcgg cgcagctgaa actggcgccg
     8521 atggcacagc acaatcgcac ggtgcacggc accaacttca ccgtcgcaat cgaagccgcg
     8581 cacggcgtgt cgaccgggat ctcggcagcc gatcgggccc acacgatcca cgtcgcgtcg
     8641 cgcccgaacg caaagccgac cgacatagtg cagcccggac atgtgtttcc gctcgttgcg
     8701 cagcccggcg gtgtgctgat gcgtgccggt cacaccgagg cctgctgcga cctggcgcgg
     8761 ctcgcgggcc tcacgcccgc ggcggtgctg tgcgagatca tgaacgacga cggcacgatg
     8821 gcacgtctgc cggacctgat cccgttcgcg cagaagcacg atatgaagct cggcacgatt
     8881 gcggatctga tccactaccg cgctgcgacc gagtcgctgg tcacgcgcgt gggcagccgt
     8941 cgcgtgcgca cgctggcagg cgagttcgaa ctcgtcgcct atcgcgatgc gccgagcgcc
     9001 ggcgcgcatc tggcgctggt tttcggcacg ccgacgcccg acgtcgaaac gctggtgcgc
     9061 gtgcacgaac cgatgtccgc gctcgacctg atcgacaccg a
//
LOCUS       DBNHBY010000459         7934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_368034, whole genome shotgun sequence.
ACCESSION   DBNHBY010000459 DBNHBY010000000
VERSION     DBNHBY010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_368034"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2839)
                     /gene="glnE"
                     /locus_tag="AC16HB_11355"
     CDS             complement(<1..2839)
                     /gene="glnE"
                     /locus_tag="AC16HB_11355"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR5607432.1"
                     /translation="MTERTQVLPTASPIGTGATRALALSRFAVRALHAEVGHAATPEQ
                     LRARLEALAMEPWSRARMEAELDAALGAARDDDSRTERLGAALRVVRRRVVLGLMARD
                     IAGIASLTEVVGAVTVLAELAVQRALPVVAHELAGRFGVPCDASGLPQDLLVVAMGKG
                     GGGELNVSSDLDLIFIYDEEGETVARGEFAGARAGLSNQEFFERLGRRLIALLAEPTG
                     DGFVFRVDMRLRPNGDAGPLAVSVSMLEEYLVRQGREWERFAWLKGRLISAPVCASPD
                     ECAAQCAALDAVVRPFVFRKYLDFNALTALRDLHALIRAETQKKSARRAGGRDDHDDN
                     IKLGRGGIREIEFIAQTYQVMRGGRDARLRQRSTLVTLAWLGELDILRAATCEKLAAH
                     YEFLRRVEHALQYVDDAQTHLMPADVAGRVRIAQMIGLPSAEALTEQLTQAREYVATT
                     FDAIFLAPEHAAARDEATADTDITVERLRALRYAQPDESAARIVGLLASRRVASATEA
                     SRTAIQRLLARAVESIGALDSEAGPDEVLARLARMIEAIAGRSTYIALLSQYPRAFER
                     VLRVLAASRWAADYLTQHPILLDELIDERLTALTNETPVDFSGWSADLNSHLDALGDD
                     VEHQMNLVRDEHHAQVFRLLVADLDGRLTVERLADHLSLLADCVLDAVLAHVWRALPK
                     RHDAAMHFAVIGYGKYGGKELGYVSDLDLVFVYDDPHPEAELTYITLARRVLLWLSTQ
                     TSSGTLFDVDLRLRPDGEKGLLVSPFAALERYQRNEDGHGAWMWEHQALTRARFCAGD
                     RVLGQRFEGLRAEVLARPRDPALLAAEVLKMRKRMHDGHPNRSGLFDLKHDDGGMVDV
                     EFIVQYLVLAHAHRHARLLGNLGNIALLRIAGELGLIEPALATAVGNAYREYRRLQHR
                     LRLNGSEYARVPRAEVQQHIDAVIGLWRAVFG"
     gene            2966..7072
                     /locus_tag="AC16HB_11360"
     CDS             2966..7072
                     /locus_tag="AC16HB_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR5607433.1"
                     /translation="MPPDDRSDSAAAASAPVAALHALENKVEYAVEEALQVAERSLAR
                     RFGAGCVRGIRGTLRALWIALIVGYFAFGALWLATRYYLMPRIDQYRPALEREASRLL
                     DADVRIAHIESGWIGFNPLLRLTNVQIVDHSGHAALTLPSLQASLSWTSVPTLQLRFR
                     DLWVLTPELDVERRADGRFVVAGFTIDPHAARSDARVADWVLSQPHIGIRHARVRYRD
                     AVADDAVDLEDVDFEWRRGWLDNRFALRARPPAALAATLDLRGAYRRPWLESATDVSQ
                     WTGRVFAQTDYADVARAARLVHALPAGVTIARAQGALRAWLDFDRGQVESAAVDLALV
                     DVSTQLGAKLEPLHFERLQGRVEAREWGTLWEGGQEFAARQLTLIGEDLALPPTDARM
                     RITRAADDKPERGLVEASGVSLTTVSALAAHLPLPGSWHDIARRFGVRGDLADLRYTW
                     EGEANAPTQYSLRTRFERLSMNSEPADPPLTATGRPRPGRPGFENLSGVLELDQRGGT
                     AQVRAREAVLDFPGIFEERLPFERLNANGRWTVRGGFELKVESLAAASPDLELTGQAT
                     YRSGGKGLGVIDASGQIARIEANRMYKMMPLQVAEGVRTWLRDALVAGTAREASFRLK
                     GDLMDFPFAQPALGDFRVTAKIVGGTLDYLPPHADDTGATATAATWPRITGIDGDFLF
                     ERRKIEITARRAQVFGVQLAGVRATIPELGAADLKLLIDGKGSGPLADYFRFVNASPV
                     GGWLGGFLARASGTGNGALSLSLDIPLRHARDARVRGAVTLANSDVNLRDDLPPFSRV
                     NGTVEFGEKSLRIAGLNGQFLGGPFRADAATRPDGAIEITGSGTAAAQAARRLIDAAA
                     VQRLIDRAQGTARYTANVVVKNRRTDLRITSDLVGLAIDLPDPLRKAADDSLPLRIEV
                     APRDGGGKAADIDAIRVTAGTRLALALERTRDASGAPRIERGAFALGDIDALALPERG
                     VRATVNVNRLDVDRWWPVLDVRSGDGSLPLDAVAARVRELVVGGKSFANVVLGATRED
                     DGLWLANVNSDHASGAVIWRPAQGGGQGRISARLARLTIPEGSRSQLTDLLDAPPTEL
                     PAFDVVANEFEYAGRTLGRLELQAQNVGAGPTAVWQVGKLEFDNPDGRLAASGQWQRA
                     SEGAPRRVAMNLALDFSDAGKLLARFGMPGALRGGEGRLEGELAWRGSPFALDYPSLA
                     GKLKLTTSKGQFLKADPGVARLLGVLSLQALPRRIGLDFRDVFSEGFAFDAITATANV
                     AGGVLSTQDFKMRGVNATVLIEGSADLRQETQNLHVLVLPEVNAGSASLAYALLNPAI
                     GLGTFVAQWLLRDPLSKAFSHEFDITGTWSDPQVRRRERTPEAAAAPK"
     gene            7075..7911
                     /locus_tag="AC16HB_11365"
     CDS             7075..7911
                     /locus_tag="AC16HB_11365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5607434.1"
                     /translation="MSNGRSVPQTVRVAAVQMVSKPAVDANLRDAEALIAEAVRAGAQ
                     LVALPEYFCLMGRETDKVAVREPDGSGPIQEFLAAAARAHRIWLVGGTVPLEASDAHR
                     VMNTVLVFDPHGVRIARYDKIHLFAFAKGDESYDEARTIAPGRAVRTFTFQPSGGPQV
                     KAGLSVCYDLRFPELYRALEQPDLILVPSAFTATTGKAQWETLLRARAIENLCYVLAP
                     AQGGKHENGRATYGHSMLIDPWGEIVASRDEGPGVVIGDVDLARIADVRASLPALAHR
                     VL"
BASE COUNT         1223 a         2775 c         2739 g         1197 t
ORIGIN      
        1 tgccgaatac cgcccgccac aacccgatca cggcatcgat gtgctgctgc acctcggcac
       61 gcggcacgcg cgcgtactcg gagccgttca gccgcagccg gtgctgcagc cgccggtact
      121 cgcgataggc gttcccgacc gcagtcgcga gtgccggttc gatcaatccg agttcgcccg
      181 caatgcgcag cagcgcgatg ttgccgaggt tgccgagcag ccgtgcatgc cggtgcgcgt
      241 gcgcgagaac gaggtactgc acgatgaatt cgacgtcgac catgccgccg tcatcgtgct
      301 tcaggtcgaa caagccggag cgattcgggt ggccgtcgtg catgcgcttg cgcatcttca
      361 gcacctcggc agcgagcagc gccgggtcgc gcggtcgcgc cagcacttcg gcgcgcagcc
      421 cttcgaagcg ctgccccaga actcgatcgc cggcgcagaa acgcgcgcgc gtcagcgcct
      481 ggtgctccca catccacgcg ccgtggccgt cctcgttgcg ctggtagcgc tcgagcgcgg
      541 cgaacggcga caccagcagc cccttttccc cgtcgggccg cagcctcaga tccacgtcaa
      601 acagcgtgcc gctcgaggtc tgcgtcgaca gccacagcag cacgcggcgc gcgagcgtga
      661 tgtaggtcag ttccgcttcc gggtgcggat cgtcatagac gaacacgaga tcgaggtccg
      721 acacgtagcc cagttccttg ccgccgtact tgccgtagcc gatcaccgcg aagtgcatcg
      781 cggcgtcgtg ccgcttcggt agtgcgcgcc acacgtgcgc cagtacggcg tcgagcacgc
      841 agtcggcgag cagcgacagg tgatcggcca accgctcgac ggtcagacgc ccgtcgaggt
      901 cggccaccag cagccggaat acctgcgcat gatgctcgtc gcgcaccagg ttcatctggt
      961 gctcgacgtc gtcgcccagc gcgtcgagat gcgagttcag gtccgcgctc catcccgaga
     1021 agtcgaccgg tgtctcgttg gtcagcgcgg tcaggcgctc gtcgatcagc tcgtcgagca
     1081 ggatcggatg ctgtgtcagg tagtccgccg cccagcggct ggcagcgagc acgcgcagca
     1141 cgcgctcgaa cgcgcgcggg tactggctga gcagcgcgat gtaggtgctg cggccggcga
     1201 tcgcctcgat cattcgcgcc agccgcgcca gcacctcgtc cggacccgct tcggagtcga
     1261 gcgcgccgat cgactcgacc gcgcgcgcga gcagacgctg gatcgcggtg cgcgacgcct
     1321 ccgtcgccga cgccacgcgg cgcgaggcca gcaggccgac gatgcgtgcc gccgattcgt
     1381 ccggctgcgc atagcgcagg gcgcgcagcc gttcgaccgt gatgtccgtg tcggcggtcg
     1441 cctcgtcccg cgcggccgcg tgctccggcg cgagaaagat cgcgtcgaac gtcgtggcga
     1501 cgtactcgcg tgcctgtgtc agctgctcag tcagcgcctc tgccgacggc aggccgatca
     1561 tctgcgcgat gcgcacgcgt cctgccacgt cggccggcat caggtgcgtc tgcgcatcgt
     1621 cgacgtactg cagcgcgtgc tcgacgcggc ggaggaattc gtagtgggcg gccagcttct
     1681 cgcaggtcgc cgcgcgcagg atgtcgagct cgcccagcca cgccagcgtg acgagcgtcg
     1741 aacgctggcg cagtcgcgca tcgcgaccgc cgcgcatcac ctggtaggtc tgcgcgatga
     1801 actcgatctc gcggatgccg ccgcggccga gcttgatgtt gtcgtcgtga tcatcgcggc
     1861 cgcccgcacg gcgtgcgctc ttcttctggg tctcggcgcg gatcagcgca tgcaggtcgc
     1921 gcagtgcggt gagcgcgttg aagtcgaggt acttgcggaa cacgaacggc cgcaccaccg
     1981 catcgagcgc ggcgcactgc gccgcgcact cgtcaggcga cgcgcacacc ggcgccgaga
     2041 tcaaccgccc cttcagccat gcgaagcgct cccactcgcg cccctgccgc accaggtact
     2101 cctcgagcat cgacaccgac accgccaacg gcccggcgtc gccgttgggc cgcagccgca
     2161 tgtcgacgcg gaacacgaaa ccgtcgccgg tcggctcggc gagcagcgcg atcagccgcc
     2221 gtccgagccg ctcgaagaat tcctggttcg acagtccggc gcgcgcgccg gcaaactcgc
     2281 cgcgtgcgac cgtctcgccc tcctcgtcgt agatgaagat caggtcgagg tcggacgaca
     2341 cgttgagctc cccgccgccg ccctttccca tcgcgacgac cagtaggtcc tgcggcaggc
     2401 cgctcgcgtc acacggcacg ccgaagcgcc ccgccagctc gtgcgcgacc accggcaagg
     2461 cgcgctgcac ggcgagttcg gcgagcacgg tcaccgcacc gacgacctcg gtcaacgacg
     2521 cgatgcccgc gatgtcgcgc gccatcagtc cgagcaccac acgccgacgc actacgcgca
     2581 gcgcagcgcc aagccgttcc gtgcgcgagt cgtcgtcgcg cgcggcgccg agcgccgcat
     2641 cgagctccgc ctccatccgc gcgcgcgacc acggttccat cgccagcgct tccagccgcg
     2701 cccgcagttg ctcgggcgtt gccgcatggc cgacctcggc gtgcagcgca cgcacggcaa
     2761 agcgcgacag cgccagcgcg cgcgtcgctc cagtgccgat cggcgaagca gtcggcaata
     2821 cttgcgtgcg ttcggtcaag gccgcgagtt ccgacgctgt gataggctgg cccggcaggt
     2881 tcatcgctga ccggattcta ggtgcacggt ggaaccaact cgtgcgccgc ggctcacata
     2941 gctgtcctcc tcccccttga accccgtgcc gcctgacgat cgctcggact ccgcggcggc
     3001 ggcaagcgcg ccggtcgcgg cattgcacgc gctcgagaac aaggtcgaat acgcggtcga
     3061 ggaagcgctg caggtcgccg agcgttcgct cgcgcgccgc ttcggtgcag gctgcgtgcg
     3121 cggtatccgc ggcacgctgc gcgcactttg gatcgcgttg atcgtcggct acttcgcgtt
     3181 cggcgcgctg tggctggcaa cgcgctacta cctgatgccg cgcatcgatc agtaccggcc
     3241 ggcactggaa cgcgaagcga gccgtctgct cgacgcggac gtgcgcatcg cacacatcga
     3301 aagcggctgg atcggcttca atccgctgct gcgcctcacc aacgtgcaga tcgtcgacca
     3361 cagcggacat gcggcgctga cgctgccgag cctgcaggcg tcgctgtcgt ggaccagcgt
     3421 gccgacgctg cagctgcgtt tccgcgacct gtgggtgctg acgcccgagc tcgacgtcga
     3481 acgtcgcgcc gatggccgct tcgtcgtggc cggcttcacg atcgacccac atgccgcacg
     3541 cagcgatgcg cgggtcgccg actgggtgct gtcgcagccg cacatcggca tccgccatgc
     3601 gcgcgtccgt tatcgcgatg cggtcgccga cgatgccgtc gatctcgagg acgtcgactt
     3661 cgaatggcgg cgcggctggc tcgacaaccg ctttgcgctg cgcgcgcggc cgccggcggc
     3721 gctcgcggcc acactcgatc tgcgcggcgc ataccggcgc ccgtggctcg agagtgcgac
     3781 cgacgtgtcg caatggaccg gccgcgtgtt cgcgcagacc gattacgccg acgtcgcgcg
     3841 cgcggcacgg ctcgtgcatg cactgccggc tggtgtgacg atcgcgcgcg cacagggcgc
     3901 gctgcgcgct tggctcgact tcgatcgcgg tcaggtcgag agcgctgcgg tggatctcgc
     3961 actggtcgat gtgtcgacgc agctcggcgc gaagctcgag ccgctgcact tcgagcggct
     4021 gcagggtcgc gtggaggctc gcgagtgggg cacgttgtgg gaaggcggcc aggagttcgc
     4081 agcgcggcag ctgacgctga tcggagagga cctcgcgctg ccgccgaccg atgcacgcat
     4141 gcgcatcacc cgcgccgcgg acgacaagcc cgagcgcggg ttggtcgagg caagcggtgt
     4201 atctctgacc acggtcagtg cgctggcggc gcacctgccg ctgccagggt catggcacga
     4261 catcgcgcgt cgctttggcg tgcgcggcga tctggccgac ctgcgctaca cgtgggaagg
     4321 cgaagcaaac gcgccgaccc agtattcgct gcgcacgcgt ttcgagcgac tgtcgatgaa
     4381 cagcgagccg gccgatccgc cgctgacggc gacagggcgg ccacgcccgg gccggccggg
     4441 cttcgagaat ctgtccggcg tgctcgaact cgaccagcgc ggcggcacgg cgcaggtgcg
     4501 tgcgcgcgaa gccgtgctgg attttcccgg catcttcgag gagcgactgc cgttcgagag
     4561 attgaacgcg aacgggcgct ggacggtgcg cggcggcttc gaattgaagg tcgaatcgct
     4621 ggcagccgcg agtcccgacc tcgaactgac tgggcaagcg acgtatcgca gcggcggcaa
     4681 ggggctcggc gtaatcgacg ccagcggcca gatcgcacgc atcgaagcga accgcatgta
     4741 caagatgatg ccgctgcagg tcgcagaagg agtgcgcacg tggctgcgcg acgcgctggt
     4801 ggccggcacc gcacgcgaag ccagctttcg gctgaagggc gacctgatgg actttccgtt
     4861 tgcgcagccg gcgctcggcg atttccgggt aacggcaaag atcgtcggcg gcacgctcga
     4921 ctacctgcca ccgcacgccg acgacactgg cgcaacggcc accgcggcca cctggccgcg
     4981 gatcaccggc atcgatggcg acttcttgtt cgaacgcagg aagatcgaga tcaccgcgcg
     5041 ccgcgcgcag gtgttcggcg tgcaactggc cggcgtgcgc gcgacgattc cggaactcgg
     5101 cgccgccgat ctcaaattgc tgatcgacgg caagggcagc ggcccgctgg ccgactactt
     5161 ccgcttcgtc aatgcgagtc cggtcggcgg ctggctcggg gggtttctgg cgcgcgcaag
     5221 cggcaccggc aacggcgcat tgagtctctc gctcgacatt ccgctgcggc acgcgcgcga
     5281 cgcgcgcgtg cgcggcgcgg tgacgctggc gaacagcgac gtgaacttgc gcgacgatct
     5341 gccgccgttt tcgcgcgtca acggcaccgt ggagttcggc gagaagtcgc tgcgcatcgc
     5401 gggtctgaac ggtcagtttc tcggcggacc gttccgcgcc gatgcggcca cgcgtcccga
     5461 cggcgccatc gagatcaccg gcagcggcac ggccgcggca caggcggcgc gccgtctgat
     5521 cgacgccgcc gcagtgcagc ggctgatcga ccgcgcgcag ggcacggcgc gctacacggc
     5581 caacgtggta gtgaagaacc ggcgcaccga cctgcgcatc acctccgatc tggtcggact
     5641 ggcgatcgac ctgcccgatc cgctgcgcaa ggcggcggac gattccctgc cgctgcgcat
     5701 cgaggttgcg ccgcgcgatg ggggcggaaa ggctgcggac atcgatgcaa tacgcgtgac
     5761 ggccggcacg cggctcgcac ttgcactcga acgcacacgc gacgcaagcg gcgcgccgcg
     5821 catcgagcgc ggcgcgttcg cactcgggga catcgatgcg ctggcgctgc cggaacgcgg
     5881 cgtccgcgcg acggtcaacg tgaatcgact cgacgtcgat cgctggtggc ctgtcctgga
     5941 cgtccgcagc ggcgacggca gcctcccgct cgacgcggtg gcggcacgcg tgcgcgaact
     6001 cgtggtcggc ggcaagtcgt ttgcgaacgt cgtcctcggt gccacgcgcg aggatgacgg
     6061 actatggctt gccaacgtga actcggatca cgccagcggc gcggtcatat ggcgtcctgc
     6121 ccaagggggt ggtcaagggc gcatcagcgc gcggctcgcg cggctgacga ttccggaggg
     6181 cagccgctcg cagttgaccg atctgctcga tgcgccgccg accgaactgc cggcgttcga
     6241 tgtggtggcg aacgagttcg agtacgcagg ccgtacgctc gggcgcctcg aactgcaggc
     6301 gcagaacgtc ggcgccgggc caacggccgt gtggcaggtc ggcaagctcg aattcgacaa
     6361 ccccgacggc cgcctcgccg ccagcggcca gtggcagcgc gcgtccgagg gcgcgccgcg
     6421 ccgcgttgcg atgaatctcg cgctcgactt ctccgacgcc ggcaagctgc tcgcgcgctt
     6481 cgggatgccg ggtgcgctgc gcggcggcga gggtcgactc gaaggcgaac tcgcatggcg
     6541 cggctcgccg ttcgcgctcg actatccgag cctggcaggc aagctgaagc tcacgacgag
     6601 caagggccag ttcctgaagg ccgaccccgg cgtggcgcgc ctgctcggcg tgctgtcgct
     6661 gcaggcgctg ccgcgccgga tcggcctcga cttccgcgat gtcttctccg aaggcttcgc
     6721 gttcgacgcg atcacggcga ccgcaaacgt cgccggcggc gtgctcagca cgcaggattt
     6781 caagatgcgc ggcgtcaacg caaccgtgct gatcgaagga tcggcggacc tgcggcagga
     6841 gacgcagaac ctgcatgttc tggtgctgcc cgaggtgaac gcgggctccg cctcgctcgc
     6901 gtatgctttg ctgaatccgg ccatcggcct ggggacgttc gttgcgcagt ggctgctgcg
     6961 cgatccgctg tcgaaggcgt tctcgcacga attcgacatc accggcacat ggagcgaccc
     7021 gcaggtgcga cgccgcgaac gcacacccga agccgcagcc gccccgaaat agccatgtcc
     7081 aacggccgtt ccgtcccaca gaccgtccgc gttgccgccg tgcagatggt gtcgaagccc
     7141 gcggtcgacg ccaatctgcg cgacgccgag gcactgatcg cggaagccgt gcgtgccggc
     7201 gcacagctgg tcgcgctgcc cgagtacttc tgcctgatgg ggcgcgagac cgacaaggtc
     7261 gcggtacgcg agcccgacgg cagcggcccg atccaggagt tcctcgccgc agcggcgcgc
     7321 gcgcaccgca tctggctggt cggcggcacc gttccgctgg aagcgtccga cgcgcaccgc
     7381 gtgatgaaca ccgtgctggt gttcgacccg catggcgtgc gcatcgcgcg ctacgacaag
     7441 atccacctgt tcgcattcgc caagggtgac gagtcgtacg acgaggcgcg cacgatcgcg
     7501 ccgggccgcg cggtgcgcac gttcacgttc cagccgtcgg gcggcccgca ggtgaaggcc
     7561 ggattgtcgg tctgctacga cctgcgcttt ccagagctgt atcgcgcgct ggaacagccc
     7621 gatctgatcc tggtgccatc ggcgttcacc gcgacgacag gcaaggcgca gtgggaaacg
     7681 ctgctgcgcg cgcgcgcgat cgaaaacctg tgttatgtgc tggcgcccgc gcaaggcggc
     7741 aaacacgaga acggccgcgc cacctacggc cacagcatgc tgatcgatcc gtggggcgag
     7801 attgtcgcca gccgcgacga aggccccggc gtggtgatcg gcgacgtcga cctcgcgcgc
     7861 atcgccgacg tgcgtgcgag cctgccggca cttgcgcatc gggtgttgtg acccgcgctc
     7921 caactgcata tcga
//
LOCUS       DBNHBY010000460         5145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_909056, whole genome shotgun sequence.
ACCESSION   DBNHBY010000460 DBNHBY010000000
VERSION     DBNHBY010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_909056"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16HB_11370"
     CDS             <1..275
                     /locus_tag="AC16HB_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5607435.1"
                     /translation="QRSQQRAMLARPDAHGILSRVRVPTLVLCGRGDPVTPVAGHEAT
                     ATCLPGVRLELIEDCGHLSTLERPYAVSRVLIAWLAETDARLRLPG"
     gene            complement(288..842)
                     /locus_tag="AC16HB_11375"
     CDS             complement(288..842)
                     /locus_tag="AC16HB_11375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR5607436.1"
                     /translation="MRLHRDGSVLLVIDIQQKLAPHVAGQRDIIRRTEALMQAARLFE
                     IPRLLTEHCPNQIGAVIEPLRSQFSADEIFVKTAFSATDHAEFIDRVHATKRRQIVMT
                     GMEAHVCVLQAALGLQQQGFHVFVVTDAIGSRGVRALDRDLALRRMEQAGCVLTTTET
                     VLFEWTQQGTDARFRDVLALVKSL"
     gene            complement(845..2434)
                     /locus_tag="AC16HB_11380"
     CDS             complement(845..2434)
                     /locus_tag="AC16HB_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HIR5607437.1"
                     /translation="MLMSADAYRESLRRIQPVVYVEGDRIESVADEPRLAPGVNAVAK
                     TYELGLRADLANLMQAHVPGFATPVNRMIALTQTTADLLNKLEAARLVCQETGCAQRY
                     LVGDVLNALFVGTHAIDAEHGTGYHVRLREYIAHVYENDLTLGVAMTDGKGDRSLRPH
                     RQPNPDTYVRIVERNARGIVISGTKAIVTGAPYVHELVVLPCRAMTEADRDFAVACAV
                     PLEAEGLTIVARPAGRPGEAAAKFSAKFGQSTGVCLFERVFVPWERVFLAGEHAGAAS
                     ILYDYTSQHRHTCIGARAGFGDLLIGAGALMIEANGLDLDRNYNLRDAMVELIKIVEG
                     FFACGVAASVYGTRNAAGNITPEPVYANIGKLLLSTQIYDMHRLAHQVSGGLIVTLPG
                     PDEDHNPAIAGRLSEVLTARSDIPYERRIEVARFVEDLTASYQAGWYSVISLHGGGSP
                     DAMKTEIYRNYPIGNKVDLVEKLLDRGVLADQTRTITRNRQPARCCVTGCQMTELAPT
                     ATLPWPIARGSAPVDEPSLAK"
     gene            complement(2466..3689)
                     /locus_tag="AC16HB_11385"
     CDS             complement(2466..3689)
                     /locus_tag="AC16HB_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607438.1"
                     /translation="MHARRIVFGLALASFVLSFFHRTAPAAIAGELTRAFSISGAVLG
                     TLAATYFYVYTVLQIPVGVLADTLGPRKLLTGGSAIAAAGSLLFALAPAWEIAAVGRT
                     LVGVGVAVAFISILKVSAVWFEHERFATLNGVTMFAGNLGAVVAGAPLAWVVSMTSWR
                     VVFVALAVLSALLAIGTWLRLRDRPEELGYPPVHALAAPSAHVPWTSALANVLANPVT
                     WPAFFVNVGVGGSYLAFAGLWAVPYLQQVYGLSRVEAAQHASLLLLGVAFGSVAIGVA
                     SDRLRSRAGVMRVAVWLYALSWAPWVLHAQWPLAATLAWFLLMGLLIPGFTLSWTIAK
                     EANPPQYSGIATSVVNLGIFLGTGILQPWVGWVLDRGRAAGDATGAWDGGIALLAGAA
                     AMSAVMTLFVGRRRE"
     gene            3785..4666
                     /locus_tag="AC16HB_11390"
     CDS             3785..4666
                     /locus_tag="AC16HB_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5607439.1"
                     /translation="MSASPELAVNLRRHVECLAGKIGERHVRRPQALHAAEAYIADEF
                     GALGYEVARQTYRAEGVASSNLEVAVPGMARASEIVLVGAHYDTVPGSPGADDNASGV
                     AALIEIARLLREGRCACTVKLVAFVNEEPPFFYWGEMGSHVCARAARARGDDIRVMLS
                     LEMLGCYDDAAGSQEYPPLLGFFYPDAGNFVAFVSNLRSRRALARLASAFKGASDFPA
                     EKLASPAIVPGVSWSDQLSFWRAGYPAVMVTDTAFYRYRHYHAATDTPEKLDYPRMAH
                     VVDGLAKAVRTLANEGT"
     gene            complement(4965..>5145)
                     /locus_tag="AC16HB_11395"
     CDS             complement(4965..>5145)
                     /locus_tag="AC16HB_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607440.1"
                     /translation="VVEIAFNDLQASPQYPGGLALRFARVKSYRPDKRADEADTIDTV
                     RAIYAEQMARAGRAF"
BASE COUNT          859 a         1817 c         1693 g          776 t
ORIGIN      
        1 ggcaacgcag tcagcaacgg gcgatgcttg cgcggccgga tgcgcacgga atcctttcgc
       61 gcgtgcgcgt gccgacgctg gtcctgtgcg gccgcggcga cccggtcacg cccgtcgcgg
      121 gccacgaggc aacggccacc tgcctgccgg gcgttcgcct cgaactcatc gaagactgcg
      181 gccacctgtc cacgctcgaa cggccgtacg cggtcagtcg cgtactgatc gcctggctgg
      241 ccgaaaccga tgcgaggctg cgtttgcccg gttgacaggc gttcgcgcta cagcgacttg
      301 accagcgcca gcacatcgcg gaagcgcgcg tcggtgccct gctgcgtcca ctcgaacaac
      361 acggtttcgg tcgtcgtcag cacgcaaccc gcctgctcca tgcgccgcag cgccaggtcg
      421 cggtcgagcg cgcgcacgcc gcgcgagccg atcgcgtcgg tgaccacgaa cacgtgaaac
      481 ccctgttgct gcagaccgag cgcggcctgc agcacgcaca cgtgcgcttc catgccggtc
      541 atcacgatct ggcgccgctt cgttgcgtgc acgcggtcga tgaattcggc gtggtcggtc
      601 gcactgaatg ccgtcttcac gaagatctcg tcggcgctga actgcgaacg cagcggttcg
      661 atcaccgcgc cgatctgatt cgggcagtgc tcggtcaaca ggcgcgggat ttcgaacagc
      721 cgcgccgcct gcatcagcgc ctcggttcgg cgaatgatgt cccgctgtcc agcgacgtgc
      781 ggtgcgagtt tctgctgaat gtcgatcacc agcagaacgg agccgtcacg atgaagtcgc
      841 atggctactt cgccaggctc ggttcgtcga ccggcgcgga cccgcgcgca atcggccacg
      901 gcagcgttgc cgtcggcgcg agttccgtca tctggcagcc cgtcacgcag catcgcgccg
      961 gctgccggtt gcgcgtgatc gtgcgcgtct ggtcggccag cacgccgcga tcgagcagct
     1021 tctcgacgag atcgaccttg ttgccgatcg gatagttgcg atagatctcg gtcttcatcg
     1081 catcgggcga accgccgccg tgcaggctga tcaccgagta ccagccggcc tggtacgacg
     1141 cggtcaggtc ctcgacgaag cgcgccactt cgatgcgcct ctcgtaagga atgtcgctgc
     1201 gcgcggtcag cacctcggac agccgccccg cgatggccgg gttgtggtcc tcgtccggcc
     1261 ccggcagcgt gacgatcagt ccaccggaca cctggtgcgc gagccggtgc atgtcgtaga
     1321 tctgtgtcga caacagcagc ttgccgatgt tcgcgtacac cggttccggc gtgatgttgc
     1381 cggccgcgtt cctcgtgccg tagacggacg ccgccacgcc gcaggcgaag aacccctcga
     1441 cgatcttgat cagctcgacc atcgcatcgc gcaggttgta gttgcggtcg agatcgagcc
     1501 cgttggcttc gatcatcagc gcgccggccc cgatcagcag gtcgccgaag ccggcacgcg
     1561 cgccgatgca cgtgtggcgg tgctggctgg tgtagtcgta cagaatcgat gccgccccgg
     1621 catgttcgcc ggcgaggaac acgcgctccc acggaacgaa cacgcgctcg aacaggcaca
     1681 cgccggtcga ctggccgaac ttcgcgctga acttcgccgc cgcttcgccc ggccggcctg
     1741 cggggcgggc gacgatcgtc agcccctctg cctcgagcgg caccgcacac gcgaccgcga
     1801 agtcgcgatc ggcctcggtc atcgcacggc atggcagcac gaccagctca tgcacgtacg
     1861 gcgcaccggt gacgatcgcc ttcgtgcccg agatcacgat gccgcgcgcg ttccgctcga
     1921 cgatgcggac ataagtgtcg gggttcggct gccggtgcgg tcgcagcgag cggtcgccct
     1981 tgccgtcggt catcgccacg ccgagcgtca ggtcgttctc gtaaacgtgc gcgatgtatt
     2041 cccgcagccg cacgtggtat ccggttccgt gctcggcgtc gatcgcgtgc gtgccgacga
     2101 agagcgcgtt cagcacatcg ccgacgagat agcgctgcgc gcagccggtt tcctggcaca
     2161 ccagccgcgc ggcctccagc ttgttcagaa ggtccgccgt cgtctgcgtc agcgcgatca
     2221 tccggttcac cggcgtggcg aagccgggca cgtgcgcctg catcaggttc gcaaggtcgg
     2281 cacgcaggcc gagttcgtag gtcttcgcca cggcattgac gccgggtgca agccgcggct
     2341 cgtcggccac gctctcaatg cggtcgcctt cgacgtagac gaccggctgg atacgccgca
     2401 gcgattcgcg ataggcgtcg gcggacatca gcatcggact ctccttgcgc gggcgacgag
     2461 ggactttact cccgccgcct gccgacgaag agcgtcatca cagcgctcat cgccgccgcg
     2521 cccgccagca gcgcgatgcc gccgtcccac gcgcccgtcg catcgccggc cgcgcggccg
     2581 cggtcgagca cccagccgac ccacggctgc aggatgccgg tgccgagaaa gatgccgaga
     2641 ttgaccaccg aggttgcgat gcccgagtac tgcggcggat tggcttcctt tgcgatcgtc
     2701 catgagaggg tgaagccggg gatcagcagg cccatcagca ggaaccacgc cagcgtcgcc
     2761 gcgagcggcc attgcgcgtg cagcacccat ggtgcccacg acagcgcgta gagccacacg
     2821 gccacgcgca tcaccccggc ccggctgcgc aggcgatccg aggccacacc gattgccacc
     2881 gagccgaacg ccacgccgag cagaagcagg ctcgcatgct gcgcggcctc gacgcgcgac
     2941 agcccgtaga cctgctgcag atacggcacc gcccacagcc cggcgaatgc gagataactg
     3001 ccgccgacgc cgacattgac gaagaacgcc ggccacgtga ccggattggc gagcacgttc
     3061 gccagcgcgc tggtccacgg cacgtgcgcc gacggcgccg ccagcgcgtg caccggcgga
     3121 tagcccagtt cttccggccg gtcgcgcagc cgcagccaag tgccgatcgc cagcaatgcg
     3181 gaaagcaccg cgagcgcgac gaacaccacg cgccacgacg tcatcgaaac gacccatgcc
     3241 agcggcgcgc cggcgaccac cgcgccgagg ttgccggcga acatcgtcac gccgttgagc
     3301 gtggcgaagc gctcgtgctc gaaccacacg gcggagacct tgaggatcga gatgaaagcc
     3361 accgccacgc cgacaccgac gagcgtccga ccgaccgcgg cgatctccca cgccggcgcg
     3421 agcgcgaaca gtagcgagcc cgcggccgcg attgccgatc cgcctgtcag cagcttgcgc
     3481 ggccccagcg tgtcggcgag cacgccaacc gggatctgca gcaccgtgta gacgtagaag
     3541 taggtggccg ccagcgtacc gagcacggcg ccgctgatcg agaacgcgcg cgtcagctcc
     3601 cccgcaatcg ccgccggcgc ggtgcggtgg aagaacgaca gcacgaagct cgcgagcgcg
     3661 agcccgaaca cgatccggcg cgcatgcacg taagcgacca tgctggaata tctgaagcaa
     3721 tgaacgaggc gcaagaatgc ccgacccgcg ctgacccgtc gacatgtgcc ggacttcggt
     3781 tgaaatgtct gcatcgcccg aacttgcggt caacctgcgc cggcatgtcg aatgcctggc
     3841 aggaaagatc ggcgagcgcc atgtgcgccg gccgcaagcg ctgcacgccg cggaggctta
     3901 catcgcggac gagttcggcg cactcgggta cgaagtcgcc cgtcagacct atcgggccga
     3961 gggcgtggcc tcgtccaacc tcgaagtcgc ggtgccaggg atggcccgcg caagcgaaat
     4021 cgtgctggtc ggcgcgcatt acgacaccgt gcccggctcc cccggtgcgg acgacaacgc
     4081 gagcggtgtc gcggcgctga tcgagatcgc gcgcctgctg cgcgaagggc gttgcgcgtg
     4141 cacggtgaag ctcgtggcct tcgtcaacga ggagccaccg ttcttctact ggggcgagat
     4201 gggcagccac gtgtgtgcgc gcgccgcgcg cgcccgcggc gatgacatcc gcgtgatgct
     4261 gtcgctcgaa atgctcggct gctacgacga cgccgcgggc agtcaggagt atccgccgct
     4321 gctcggattc ttctatcccg acgccggcaa cttcgtcgcg ttcgtctcca acctgcgctc
     4381 gcggcgcgcg ctcgcccggc tcgcgagcgc cttcaagggc gcatcggact ttccggctga
     4441 aaagcttgcc agccccgcga tcgtgccggg cgtgtcctgg agcgaccagc tttcgttctg
     4501 gcgcgcgggc tatccggccg tgatggtcac cgacacggcg ttctatcgct atcgccacta
     4561 ccacgccgcg accgacacgc ccgagaaact cgactaccca cgcatggccc acgtagtcga
     4621 tggactggcg aaggccgtgc ggacactggc gaacgaagga acctagccga tcgtcgatgc
     4681 ggtccgcgaa cggaaggcgc gtccgcgtcg gagcctgcgg cttcgcaccg gcgcggaacg
     4741 ccgactctgc gactgtcgac tgccagaaca gctgccgacc ttccaccagc agccgcggcc
     4801 cacctgcagc aactcgccga cacgcgctgt gtcgagcgcg ccgacggaat gtgtggccag
     4861 cgcgaacgcc ggcgctctaa ggagcgcgat ctatcttgcg agccggaacc actcgtgcgg
     4921 atccgtcttg acccgaacca gaatgccgtc agggccgacg agcctcagaa cgcgcggccg
     4981 gcgcgcgcca tctgctcggc gtagatcgcg cgaaccgtat cgatcgtatc ggcctcgtcg
     5041 gcgcgcttgt cgggccggta gctcttcacg cgcgcgaagc gcagcgcaag cccgcccggg
     5101 tactgcgggc tcgcctgcag gtcgttgaac gcgatctcga ccacg
//
LOCUS       DBNHBY010000461         3622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1248386, whole genome shotgun sequence.
ACCESSION   DBNHBY010000461 DBNHBY010000000
VERSION     DBNHBY010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1248386"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1109)
                     /locus_tag="AC16HB_11400"
     CDS             complement(120..1109)
                     /locus_tag="AC16HB_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607441.1"
                     /translation="MKRRTILSAAALGGASALARAQPAATRPAHAWPTRPLRMILTAP
                     PGSSIDVLGRLIAERLRERLGQPVIADNRAQAGGTVGTDAIAKADHDGTTFGLSFTGP
                     LATAPSLYSKLPYDPSRDLAPIVLVGTAPNLLAVNAALPVGNFKEFVLYARARPGRLS
                     YASIGNGSSSHLAMELLKAQANLFLLHIPYNGAPPAVQAVAAGEVQAIMSNPTSLLPL
                     IHAGRIRPVAVTSRARWAPMKELPTIAESGFPGFEAIAWNGFVAPAGTPRDVIERLNR
                     EINAVLQAPDTKTRIEAAGWDVAGGSAESFAVLMRAERERWAPVIRRSGARID"
     gene            complement(1141..2790)
                     /locus_tag="AC16HB_11405"
     CDS             complement(1141..2790)
                     /locus_tag="AC16HB_11405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5607442.1"
                     /translation="MPFSTLSAPLDTQAALEEAWARVLTAPQRSIAVADAWSNDEEPP
                     GFNSLFARVILAAARGRYTARAGSLADLEAVLRSLDRATLTHSDRARLQALARGVHGM
                     LHTIDRDVDSALADLNAALASTPALPSYDRHCLHVWRAVALMASAKPELAFHDFFAEY
                     EYVHAHHPAVFALLLLNIGAVLLHVGDWEGAESSLQQALAAESLVEIAGFGTVCRVNL
                     AYCFLQTGRVEPARRLVLELLARDRSFLLQRHPGDVLATVAEDLIETGCFAEAEQYLH
                     GLLEDAQMRGFRLGLATGAWSSGRLAFLRGDVAQAVAHWRRAALLLRRLPHLPHLWKT
                     LLAISDFYARRGDWRRAWRWQQRYHTAHQRWSAASQPARLAYARAALELRLTREQAIR
                     DPVTGLLNRRELLDRLEQLIAAARVDKSPLTVGMIDLDNLKPINDRYGHRIGDSAIVF
                     AAERLRMLLPPEAQVFRYGGDEYCALLPDCTREQAAHLFASYLDSLRAWRTPAGAERR
                     SLLSASVGLAVLNGTTRPEALLDAADTALYRAKRKGGNCIA"
     gene            complement(2934..>3622)
                     /gene="argF"
                     /locus_tag="AC16HB_11410"
     CDS             complement(2934..>3622)
                     /gene="argF"
                     /locus_tag="AC16HB_11410"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5607443.1"
                     /translation="GDSQLGRAEPIEDTARVISRMVDVVMIRTYEQAKIERFAAYSRV
                     PVINGLTDEYHPCQILADIYTYIEHRGSITGRTVAWIGDANNMAYTWLQAAQLLGFAL
                     HVSTPQEYSLDARLASPGSHFRHFADPRDATRGADLVTTDVWTSMGFEAENEARRRAF
                     ARWQVDANLMQSAKPDALFMHCLPAHRGEEVTADVLEGPQSVVFDEAENRLHAQKALM
                     EYLLLGRSVR"
BASE COUNT          573 a         1212 c         1267 g          570 t
ORIGIN      
        1 tgcgcatcca cagcagtgcc gcgctggtgt cgagtccgcc ggaaaacgca atgccgacct
       61 tgtgcccggt cgggagtgac tgcaggatat tggccatgcc gcgattctat ccggcgcgat
      121 cagtcgatcc tggcgccgct gcgcctgatg accggcgccc agcgctcgcg ctcggcgcgc
      181 atgagcacgg cgaaagactc tgcgcttccg ccggccacgt cccagccggc ggcttcgatg
      241 cgcgttttcg tgtcgggcgc ctgcaggacc gcgttgatct cgcggttcag ccgctcgatc
      301 acgtcgcgtg gagtgccggc cggcgcgacg aagccattcc acgcgatcgc ctcgaagccg
      361 gggaatccgc tttcagcgat cgtcggcagt tccttcatcg gcgcccagcg cgcgcggctc
      421 gtcactgcga ccggccggat ccgcccggcg tggatcagag gcagcagcga agtcggattg
      481 ctcatgatcg cctgaacctc gcccgccgcg accgcctgca ccgcgggcgg cgctccgttg
      541 tacggaatgt gcaagaggaa cagatttgcc tgcgccttca gcagttccat cgccaggtgc
      601 gacgaactgc cgttgccgat cgatgcatag ctgagccggc ccggccgcgc gcgggcgtac
      661 agcacgaatt ccttgaagtt cccgaccggc agcgcggcgt tgaccgcaag caggttgggc
      721 gcagtgccga ccagcacgat cggcgccaga tcgcgtgatg gatcgtaggg cagcttcgaa
      781 tacagagacg gcgcggtcgc gagcggaccg gtgaacgaca ggccgaaggt ggtgccgtcg
      841 tgatccgcct tggcgatcgc gtcggtgccg acagtaccgc cagcctgcgc tcggttgtca
      901 gcgatcacgg gttggccgag ccgttcgcgc agcctttcgg cgatcaggcg gcccagcaca
      961 tcgatcgacg agcccggcgg tgcggtcagg atcatgcgca gcgggcgtgt cggccacgca
     1021 tgagccggcc tggtcgcagc gggctgtgcg cgcgccagcg cgcttgcacc gcccagcgcg
     1081 gctgcagaga gaatcgttcg gcgtttcatg atcgggcgcg attgtcgccc ggattgcggt
     1141 tcacgcgatg cagttgccgc ccttgcgctt ggcgcggtac agcgccgtgt cggccgcgtc
     1201 gagcagcgcc tccgggcgtg tcgtcccgtt cagcaccgcc agtccgacgg acgctgaaag
     1261 cagggagcgc cgctcggcgc cggccggggt acgccaggcg cgcagcgaat cgaggtagct
     1321 ggcgaacaga tgcgccgcct gctcccgcgt gcagtccggc agcagcgcac agtattcgtc
     1381 accgccatag cggaacacct gcgcctcggg cggcagcagc atgcgcagcc gttcggcggc
     1441 gaacacgatc gcactgtcgc cgatgcgatg accgtagcgg tcgttgatcg gcttcaggtt
     1501 gtccagatcg atcatgccga ccgtcagcgg cgatttgtcc acacgcgccg cggcgatcag
     1561 ttgctcgagc cgatcgagca gttcgcgccg gttcagcagg cccgtcaccg gatcgcggat
     1621 cgcctgctcg cgcgtcagcc gcagttcgag cgcggcgcgc gcataggcga gtcgcgccgg
     1681 ctgcgatgcc gcgctccagc gttgatgcgc ggtgtggtag cgctgctgcc agcgccacgc
     1741 gcgccgccag tcgccgcggc gcgcgtagaa atcggagatc gccagcagcg tcttccacag
     1801 gtgcggcaaa tgtggcagtc gacgcagcag cagggcagca cggcgccagt gtgcaaccgc
     1861 ctgcgccacg tcgccgcgca ggaatgccag ccgcccgctc gaccacgcgc cagtggcgag
     1921 tcccagccgg aagccgcgca tttgcgcgtc ctcgaggagt ccgtgcaggt actgctcggc
     1981 ctcggcgaag cagccggttt cgatcaggtc ttccgccacc gttgccagca cgtcgccggg
     2041 gtgccgctga agcaggaagc tgcgatcgcg cgcgagcagt tcgagcacca gcctgcgcgc
     2101 cggttcgacg cgccccgtct gcaggaagca gtaggcgagg ttgacgcgac agacggtgcc
     2161 gaagccggcg atctcgacca gcgactcggc ggcgagcgcc tgctgcaggc tcgattcggc
     2221 gccctcccag tcgccgacgt gcagcagcac cgcgccgatg ttcagcagca gcagcgcgaa
     2281 cacggccgga tggtgcgcgt gcacgtactc gtactcggca aagaagtcgt ggaacgccag
     2341 ttccggcttt gccgacgcca tcagcgccac cgcgcgccag acgtgcaggc aatggcgatc
     2401 gtagctcggc agtgcgggcg tcgacgcaag cgccgcgttc agatccgcga gcgcgctgtc
     2461 cacgtcgcgg tcgatcgtgt gcagcatgcc gtgcaccccg cgcgcgagcg cctgcagtcg
     2521 tgcgcgatcg ctgtgtgtca gcgtggcccg gtcgagcgac cgcagaaccg cctcgaggtc
     2581 ggccagcgaa ccggcgcgcg ccgtgtagcg cccgcgcgcg gcggcaagga tcacgcgcgc
     2641 aaacagcgaa ttgaatccgg gcggttcttc gtcattcgac catgcatccg cgaccgcgat
     2701 cgaacgctgg ggtgcggtca ggacccgcgc ccaggcttct tcgagggcgg cctgcgtgtc
     2761 gagcggcgcg gagagcgttg agaaaggcat cggtgccaac cggtcgatgt cgatccagca
     2821 tacgacacga gaacaggctt cgggtgtcga atattcactg gacggcgacc ggctggcgcg
     2881 cggccgaaac cggggatcgg cgccgcggaa agggcaacgg ccgacgtgag tgtttaccgc
     2941 acgctgcggc cgagcagcag gtattccatc aatgccttct gcgcgtgcag cctgttttca
     3001 gcctcgtcga acaccaccga ctgcgggcct tcgagcacgt ccgcggtcac ttcttcgccg
     3061 cgatgcgcgg gcaggcagtg catgaagagg gcatccggct tggcggactg catcagattc
     3121 gcgtcgacct gccagcgggc aaaggctcgg cggcgggctt cgttctccgc ttcgaagccc
     3181 atgctggtcc acacgtccgt ggtgacgaga tcggcgccgc gcgtggcgtc gcgcggatcg
     3241 gcgaagtggc gaaagtgcga tcccggcgac gcgagccggg cgtcgagcga atactcctgc
     3301 ggcgtcgaca cgtgcagcgc gaaaccgagc agttgcgccg cctgcagcca cgtgtaggcc
     3361 atgttgttgg cgtcgccgat ccacgccacg gtcctgccgg tgatcgagcc ccggtgctcg
     3421 atgtaggtgt aaatgtcggc gaggatctgg caggggtggt attcgtcggt cagcccgttg
     3481 atcacgggca cgcgtgagta ggcggcaaac ctctcgatct tggcctgctc ataggtgcgg
     3541 atcatcacga cgtcgaccat gcgcgagatg acgcgcgcgg tgtcctcgat cggctcggcg
     3601 cggccgagct gcgaatcgcc gg
//
LOCUS       DBNHBY010000462         4203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1721496, whole genome shotgun sequence.
ACCESSION   DBNHBY010000462 DBNHBY010000000
VERSION     DBNHBY010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1721496"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..771)
                     /locus_tag="AC16HB_11415"
     CDS             complement(22..771)
                     /locus_tag="AC16HB_11415"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR5607444.1"
                     /translation="MLIRTVSARTGAMWLRDGWRLFARQPLGLSAMVVVYLMMLFVPA
                     LIPFIGIAISGVLAPFATVALLAACREVDAKRAPTVNLFAQPFQDAKVRTQLALLGMI
                     NAALVLAVAVLGTLLGGAEPSQAPSSIQDIPLDALALQLLLYTPVLMLMWFAPVLVGW
                     HRLTPGKAMFGSVVACWRNKAALLVFGVVVSLMMMAASAIVVTLVSSLFSSRQALSIV
                     LAPLALVLMTLVQASFYPMYRSVFSDGEPSR"
     gene            complement(797..1765)
                     /locus_tag="AC16HB_11420"
     CDS             complement(797..1765)
                     /locus_tag="AC16HB_11420"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR5607445.1"
                     /translation="MAVFTALTRDDIAQLLKTYTLGELLSHQGIATGIENTNYFVTTT
                     NGAFVLTIFERLTFRQLPFYLELMRHLARRDLPVPAPQESTSGSLISEVKGKPAAIVT
                     RVPGRSVETPTAAHCERVGELLANMHLAAQDFPRFQPNLRGLGWWKTVLPELERFVDD
                     VTFHALAEEVIHQDSFYRSAAFEQLPSGPIHADLFRDNVLFVERSGQPAIGGVIDFYF
                     AGCSIWLFDVAVVVNDWCVEVDDGALVMDRAQRLLRAYHAVRPLLELEREHWRTVLRA
                     AALRFWISRLHDLHLPRVAHLLQPHDPGRFEKTLKRRIADPELPWI"
     gene            complement(1838..2194)
                     /locus_tag="AC16HB_11425"
     CDS             complement(1838..2194)
                     /locus_tag="AC16HB_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607446.1"
                     /translation="MKGMPILVAVAVLAAAVARAQPLDAPPARKDAPPRVEVKGAETL
                     TEPEASELLPPKHEVHGETRIEQRRQGTRVREIVVTPAGRSYSYTIENREGRRPLSTQ
                     DLASGLSTPRFLKFEF"
     gene            complement(2213..2950)
                     /locus_tag="AC16HB_11430"
     CDS             complement(2213..2950)
                     /locus_tag="AC16HB_11430"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5607447.1"
                     /translation="MLDRQKNIPSLRAVTTPERATSQKARESWQIWGIISEFVEAAER
                     LSEIRPAVSIFGSARAKEGSPVYAQAVEIARKLSDAGFAVISGGGPGVMEAANRGAFD
                     GRSPSVGLNIELPFEQHGNAYQDISLRFRHFFARKVAFVKYAAAYVVMPGGFGTLDEL
                     SEALTLIQTRMGRTIPIVLVGSDFWAGLLAWMRSTLVGQGMIAENDLDLMKLADDSDA
                     VVDAIFDFYEKRGFATTPAEREQLLYL"
     gene            2949..>4203
                     /locus_tag="AC16HB_11435"
     CDS             2949..>4203
                     /locus_tag="AC16HB_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR5607448.1"
                     /translation="MADSEKTLLLVDGSSYLYRAFHALPDLRTSRGEPTGAIRGFISM
                     LKLLREQVPADYLACVFDAKGKTFRDDLYPDYKAHRPPMPDDLTAQIAPIHEAVEALG
                     WPVLEIEGIEADDVIGTLARHAQTHGVKTVVSTGDKDLAQLVNDKVTLVNTMNNEKLD
                     TAGVVAKFGVRPDQIVDFLALVGDSVDNVPGVEKVGPKTAAKWLQQYGSLGAIVANAD
                     QIGGVVGENLRQALDWLPTAQTLVTVKTDGDIAAHVPHFDALRARDPDTERLHDLYTR
                     WEFKSFLRELGAGSALPAPAPVAEPVSPAQPQTRYETVLTDVQLDAWLRSINAAALTC
                     IDTETTSLDPMQARLVGISLSVHENEACYIPLAHRYPGAPKQLPFDATLAKLKPWLES
                     EQHKKVGQHLKYDAHVFANHGVRLAG"
BASE COUNT          757 a         1431 c         1374 g          641 t
ORIGIN      
        1 gcgagagggg ccgagcctcg cctagcggct cggctcgccg tcgctgaaca ccgaccgata
       61 catcggatag aagctcgctt gcaccagcgt catcagcacc agtgccaggg gcgccagcac
      121 gatcgacagt gcctgccgcg acgagaacag cgacgacacc agcgtgacga cgatcgcgct
      181 ggccgccatc atcatcaggc tcaccacgac gccgaatacc agcaacgccg ccttgttgcg
      241 ccagcaggcg accacgctgc cgaacatcgc cttgccgggg gtcaaccggt gccaccccac
      301 cagcaccggc gcgaaccaca tcagcatcag caccggcgtg tacagcagga gctgcagcgc
      361 cagcgcgtcg agcggaatgt cctggatcga cgacggcgcc tgcgacggct ccgccccgcc
      421 gagcagcgta ccgagcaccg cgactgccag caccagcgcg gcattgatca tgccgagcag
      481 cgcgagttgc gtgcgcacct tggcgtcctg gaatggctgc gcgaacaggt tcaccgtcgg
      541 tgcgcgcttc gcatcgacct cgcggcacgc ggcgagcagc gcgacggtgg caaacggtgc
      601 caaaacgccc gagatcgcga tgccgatgaa cgggatcagc gccggcacga acagcatcat
      661 caggtagacg accaccatcg ccgacagtcc cagcggctgg cgcgcgaaca gacgccagcc
      721 atcgcgcagc cacatcgcgc cggtcctggc agaaacggtc ctgatcagca tgcgatcgat
      781 tctatcggcg cacggttcag atccacggca actcggggtc ggcaatgcgc cgtttgaggg
      841 ttttctcgaa tcggcccgga tcgtgcggct gcagcagatg cgccacgcgc ggcaggtgca
      901 gatcgtgcag gcgcgagatc cagaaacgca gcgcggccgc gcgcagcacg gtgcgccagt
      961 gctcccgctc cagttcgagc agcggccgca ccgcgtgata cgcccgcagc agccgctgcg
     1021 cgcgatccat caccagtgcg ccgtcgtcga cctcgacaca ccagtcgttg acaacgaccg
     1081 cgacgtcgaa cagccagatg ctgcagccgg cgaagtagaa gtcgatcacg ccgccgatcg
     1141 cgggctgtcc gcttcgctcg acgaacagca cgttgtcgcg gaacaggtca gcgtgaatcg
     1201 gtccggacgg caactgctcg aacgcggccg agcgatagaa gctgtcctgg tggatcactt
     1261 cctccgccag tgcgtgaaac gtcacgtcgt cgacgaaccg ctcgagctcc ggcagcaccg
     1321 tcttccacca gcccagcccg cgcagattcg gctgaaagcg cgggaagtcc tgcgccgcca
     1381 gatgcatatt cgcgaggagt tcgccgacgc gctcgcagtg cgcggccgtg ggcgtctcga
     1441 ccgaccgccc cggcacgcgt gtgacgatcg cggccggctt gcccttcact tccgagatca
     1501 gcgagccgct ggtgctttcc tgcggcgccg gcacgggcag gtcgcggcgc gccagatggc
     1561 gcatcagctc gaggtaaaag ggcagctggc gaaacgtcag acgctcgaag atcgtcagca
     1621 caaacgcgcc gttcgtggtc gtgacgaagt agttggtgtt ttcgatgccg gtcgcgatcc
     1681 cctggtgcga cagcagctcg ccgagcgtgt aggtcttcag cagctgcgcg atgtcgtcgc
     1741 gggtcagcgc ggtgaagacg gccatcagcg cgcaccccgg agcgggcggc ccacccgtgt
     1801 cacgagtggc cgcgggcggg tgatccggcg ctgcaagtca gaactcgaat ttcaggaagc
     1861 gcggggtcga caacccgctc gcgagatcct gcgtcgacag cggccgccgg ccttcgcggt
     1921 tttcgatcgt gtagctgtag ctgcgcccgg ccggcgtgac gacgatttcg cgcacgcgcg
     1981 tgccctggcg ccgctgctcg atgcgggtct cgccgtgcac ctcgtgcttc ggcggcagca
     2041 gctcggacgc ctcgggctcg gtcagggtct ccgcaccctt gacctcgacg cgcggcggcg
     2101 cgtcctttct cgcgggcggc gcatcgagcg gctgcgcccg tgccacggcg gcggccagca
     2161 cggccactgc gacgaggatc ggcatgcctt tcatgaatcc gattttacgc cgctagagat
     2221 acagcaactg ctcgcgctcg gccggggtgg tggcgaagcc gcgcttctcg tagaagtcga
     2281 agatcgcgtc gaccaccgcg tcgctgtcgt cggcgagctt catgaggtca aggtcgttct
     2341 cggcgatcat tccctgcccg accagcgtgc tccgcatcca cgccagcagc ccggcccaga
     2401 agtcggagcc gaccagcacg atcgggatcg ttcgccccat gcgggtctgg atcagcgtca
     2461 gtgcctcgct cagttcgtcc agtgtcccga aaccgcccgg catcaccacg tacgcggccg
     2521 cgtacttgac gaaagccact ttgcgcgcaa aaaagtgacg gaaccgcagc gagatgtcct
     2581 ggtatgcatt gccgtgctgc tcgaacggca gctcgatgtt caggcccacg cttgggctgc
     2641 ggccgtcgaa cgcgccccgg ttggccgcct ccatcacgcc gggaccgccg ccgctgatca
     2701 ccgcgaagcc ggcatcggag agcttgcgcg cgatttcgac cgcctgcgcg tacaccggcg
     2761 agccttcctt ggcgcgcgcg ctgccgaaga tcgacacggc tggacggatt tccgacagac
     2821 gttcggcggc ctcgacgaac tccgagataa tcccccagat ctgccacgac tcgcgcgcct
     2881 tctggctggt ggcgcgttcc ggcgtggtca ccgcgcgcag gctgggaatg tttttctgac
     2941 gatcgagcat ggctgactcc gaaaagacac tgttgctggt cgacggttca agctacctgt
     3001 accgcgcgtt tcatgcgctg cccgacctgc gcacgtcgcg cggcgagccg accggcgcga
     3061 ttcgcggctt catatcgatg ctgaagctgc tgcgcgaaca ggtgccggcg gattatctcg
     3121 cctgcgtgtt cgatgccaag gggaagacgt ttcgcgatga cctctacccc gactacaagg
     3181 cgcaccggcc accgatgccg gacgatctga ccgcgcagat cgcgccgatc cacgaggcgg
     3241 tcgaggcgct cggctggccg gtgctggaga tcgaaggaat cgaggccgac gacgtgatcg
     3301 gcacgctggc gcgccacgca cagacgcacg gcgtgaagac tgtcgtgtcg actggcgaca
     3361 aggacctcgc gcaactggtg aacgacaagg tcacgctcgt caacacgatg aacaacgaga
     3421 agctcgacac ggccggcgtc gttgccaaat tcggcgtgcg acccgaccag atcgtcgact
     3481 ttctggcgct ggtgggtgat tccgtcgaca atgtgccagg ggtggaaaag gtcgggccga
     3541 agaccgcggc caagtggctg cagcagtacg gttcgctcgg cgcgatcgtc gccaatgccg
     3601 accagatcgg tggcgtcgtc ggcgagaacc tgcggcaggc gctcgactgg ctgccgaccg
     3661 cgcagacgct ggtgaccgtg aagaccgacg gcgacatcgc ggcccatgtg ccgcacttcg
     3721 acgcgctgcg cgcacgcgac ccggatacgg agagactgca cgatttgtac acgcgctggg
     3781 agttcaagag cttcctgagg gaactcggcg caggcagcgc gctgcctgcg cccgcgccgg
     3841 tggccgagcc ggtcagcccg gcgcagccgc agacgcgcta cgaaacggta ctgaccgatg
     3901 tgcagctcga tgcctggctg cgcagcatca acgccgcggc gctgacctgc atcgacaccg
     3961 agaccacgtc actcgacccg atgcaggcaa ggctggtcgg catttcgctg tcggtgcacg
     4021 agaacgaggc ctgctacatc ccgctcgcgc atcgctatcc gggcgcacca aagcaactgc
     4081 cgttcgacgc gacgctggcg aaactgaagc cctggctcga aagcgaacag cacaagaagg
     4141 tcggccagca cctcaagtac gacgcgcacg tgttcgccaa tcatggcgtg cggctggccg
     4201 gca
//
LOCUS       DBNHBY010000463         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1585200, whole genome shotgun sequence.
ACCESSION   DBNHBY010000463 DBNHBY010000000
VERSION     DBNHBY010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1585200"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="AC16HB_11440"
     CDS             <1..744
                     /locus_tag="AC16HB_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR5607449.1"
                     /translation="LLAGCALTLLAACGTTERDPSAKWDADRLYAEAKTEIAAGNWQT
                     ARNWLEKLEARYPFGRYAQQAQMEIAYTYYKEGETAQAISACDRFLKLNPNHAAADYV
                     MYLKGLATFSDDLGLFGRSFGMDPSLRDPKAMREAFDAYKELVTRFPDSRYAADARAR
                     MNYLINAMAQSEINVARYYFLRGAYVAAIQRAQGALRDYQGTPAAEEALSILARSYGA
                     LGLDTLRADAERVLRTNFPNSRYLAAATR"
     gene            complement(720..>2573)
                     /gene="yoaA"
                     /locus_tag="AC16HB_11445"
     CDS             complement(720..>2573)
                     /gene="yoaA"
                     /locus_tag="AC16HB_11445"
                     /inference="COORDINATES: protein motif:HMM:NF053712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR5607450.1"
                     /translation="FVAEAGTGTGKTFAYLVPSLLAGGKVLVSTGTKSLQDQIFRKDV
                     ALVSHALGLKLESALLKGRQNYVCLLRLARTEADGLLATRQDVVHLRAIARFAKSTAS
                     GDRAELAEVPETATIWPAVTSTRENCLGQECPRFDDCFVYRARRVAQAADLVVVNHHL
                     FLADLALRDDAIAEFLPKVDTVVLDEAHQLPKIAADFFGNGWSLAQLADLAHDARALG
                     LHAAADGAEWIALTQWLESAGRHVRLALAERGLAPGTRIAMEKLARRDGLRDELRRVD
                     DALAALAQAVSINTGRDDELDAVAPRVTALRQAIGAWIEHEPGRDADEPGDDDFVRWV
                     GLTAHGAQFQATPLAPGETFARLREQHPQAWILTSATLTLAASFDPFLREIGLADAPA
                     QRWDSPFDFARQALLYLPQSMPSPLARDFPELVADAAWPVIVAAGGRTFLLCTTLRAV
                     ERVAEQLRRHMKRDGIELPLLVQGASTRTALLDAFRSLGNAILVGSVSFWEGIDVRGD
                     ALSLVVIDKLPFAPPDDPVVEARIRRLRARGRNPFLEYQLPQAVTLLKQGAGRLIRDE
                     NDRGVLMILDDRLLTKPYGRTVLASLPPCARTRSEQEACGFFAPQRVAAAR"
BASE COUNT          429 a          897 c          857 g          390 t
ORIGIN      
        1 ctgctggccg gctgcgcgct caccctgctc gccgcgtgcg gcacgaccga gcgcgatccg
       61 tcagccaagt gggatgccga tcgattatat gcagaggcca agacagagat agccgccggc
      121 aactggcaga cggcgcgcaa ctggctggag aaactcgaag cgcgttatcc gttcggccgc
      181 tatgcgcagc aggcgcagat ggagatcgcc tacacctact acaaggaagg cgagaccgcg
      241 caggccatct ccgcctgcga ccggttcctg aagctcaatc ccaaccatgc cgccgccgac
      301 tacgtgatgt acctgaaggg gctggcgacg ttttccgatg acctcgggct gttcggccgc
      361 tcgttcggaa tggatcccag cttgcgcgac cccaaggcga tgcgcgaggc attcgacgcc
      421 tacaaggaac tcgtcacgcg ctttcccgac agccgctatg cggcggatgc gcgcgcgcgg
      481 atgaactacc tcatcaacgc gatggcgcag agcgagatca acgtcgcccg ctactacttc
      541 ctgcgcggcg catacgtcgc cgcgatccag cgcgcccagg gggcattgcg cgactaccag
      601 ggcacgccgg cggccgagga ggcgctgtcg atcctggcac gctcgtacgg tgcgctcggc
      661 ctggacacgc tgcgcgccga cgcggagcgc gtgctgcgca cgaacttccc caacagccgt
      721 tacctcgccg ccgccacgcg ctgaggcgca aagaagccgc acgcctcctg ctcgctgcgc
      781 gtgcgcgcgc agggcggcag gctggcgagc acggtgcggc cgtagggctt cgtcagcagg
      841 cggtcgtcca ggatcatcaa cacgccgcga tcattctcgt cgcggatcag acgcccggcg
      901 ccctgcttca acagcgtgac cgcctgcggc agttgatact cgaggaatgg gttgcggccg
      961 cgcgcgcgca gtcggcggat gcgtgcttcg accaccggat catcgggcgg cgcgaagggc
     1021 agcttgtcga tcacgaccag agacagcgcg tcgccgcgca cgtcgatacc ttcccagaag
     1081 ctgacgctgc cgaccaatat cgcgttgcca agagaccgga atgcatcgag cagtgcggtg
     1141 cgcgtgctgg ctccctgcac cagcagcggc agttcgatgc cgtctcgctt catgtgccgg
     1201 cgcaattgtt cggcgacgcg ctccaccgcg cgcagcgtcg tacacagcag aaaggtccgc
     1261 ccgcccgcgg ccacgatgac aggccacgcg gcgtcggcga cgagttcggg gaaatcgcgt
     1321 gccagcgggc tcggcatcga ctgcggcagg tacagcagcg cctggcgcgc gaaatcgaaa
     1381 ggactgtccc accgctgcgc cggcgcatcg gcgagaccga tctcccgcag gaacgggtcg
     1441 aacgacgctg cgagcgtcag tgtcgccgag gtcaggatcc acgcctgcgg atgctgctca
     1501 cgcaggcggg cgaaggtctc tcccggcgcc agcggcgtcg cctggaattg cgcgccgtgc
     1561 gcggtcagtc cgacccagcg cacgaagtcg tcgtcgccgg gctcatccgc atctcggcct
     1621 ggctcatgct cgatccacgc gccaatcgcc tggcgcagtg cggtcacgcg cggtgcgacg
     1681 gcatcgagtt cgtcgtcgcg tcccgtgttg atgctcactg cctgggccag tgcggcaagt
     1741 gcgtcgtcga cccggcgcag ttcgtcgcgc agtccgtcgc gccgcgccag tttctccatc
     1801 gcaattcgcg tccccggcgc cagaccccgc tccgcgagag ccagccgcac gtgtcgaccc
     1861 gcggactcga gccactgcgt cagcgcaatc cactccgctc cgtccgccgc cgcatgcagt
     1921 ccgagcgcgc gcgcgtcgtg cgccagatca gcaagctgcg ccagcgacca gccattgccg
     1981 aagaagtcgg ctgcgatctt cggcagctgg tgcgcctcgt cgagcacgac cgtgtcgacc
     2041 ttcggcagga attcggcgat cgcgtcgtcg cgcaaggcga ggtcggcgag aaacagatga
     2101 tggttgacca ccacgaggtc ggccgcctgc gcgacgcgcc gcgcgcggta gacgaagcag
     2161 tcgtcgaagc gcgggcattc ctggccgaga cagttctcgc gcgtggatgt gaccgccggc
     2221 cagatcgttg cagtctccgg aacctcggcc agttcggcgc ggtcgccgga cgcagtcgat
     2281 ttcgcgaagc gcgcgatcgc gcgcagatgc accacgtcct ggcgcgttgc gagcaggccg
     2341 tcggcctcgg tgcgcgcgag gcgaagcagg cacacgtagt tctgccgccc cttcagcagc
     2401 gcggattcga gcttcagccc gagcgcatgc gataccagcg cgacgtcctt gcggaagatc
     2461 tgatcctgca gcgacttggt gccggtcgag acgagcacct tgccacccgc gagcagtgac
     2521 ggcacgaggt acgcgaacgt cttgccggtg cccgtgccgg cttctgcgac gaa
//
LOCUS       DBNHBY010000464         2645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1518492, whole genome shotgun sequence.
ACCESSION   DBNHBY010000464 DBNHBY010000000
VERSION     DBNHBY010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1518492"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..501)
                     /locus_tag="AC16HB_11450"
     CDS             complement(94..501)
                     /locus_tag="AC16HB_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5607451.1"
                     /translation="MALVAVLTLVCAGSALGQLRTELGKREYMSNCAVCHGTTGKGDG
                     SYFELLKTRIPDITTISQRNKGVFPADRLYQVIDGREALKSHGTREMPIWGQDYSVQA
                     GKYYIDTPYDPEVYIRVRILSLIDYIYSLQSVR"
     gene            733..1851
                     /locus_tag="AC16HB_11455"
     CDS             733..1851
                     /locus_tag="AC16HB_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5607452.1"
                     /translation="MSADRIQVVYRLHVPPAQAQERARLLAIEQSLEMPPEAVQEPRV
                     LDEIAARVERIDPQVDGTSQATVSLSVDSIGDDAGQLMNMMFGNSSLQPDVEVLDVDV
                     PVQLAAMFGGPNLGIEGLRRLAGARDLALTCSALKPIGSGIAHLSSLCSALARAGIDV
                     IKDDHGWANQASAPFTQRVAGCQRAVADANAGQSGRQTLYAPSLYGTHGQMQRQIELA
                     RKYGVKIVLIAPMVCGVATLNALRREHPDLAFLAHPALGGSARIAPPTLLGKLFRLFG
                     ADAVIFPNHGGRFTYSRAVCTAIAAHNRAEWHGMRRALPTPAGGMSLERCAEIVAEYG
                     TDTMLLIGGSLLIAREHIGERARAFVAAVRAASMPVAA"
     gene            1848..2270
                     /locus_tag="AC16HB_11460"
     CDS             1848..2270
                     /locus_tag="AC16HB_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5607453.1"
                     /translation="MSAIRRFTADPAGNFGWQDVERLRYKEEGSAPFRDITRQVLFAQ
                     PDQACELRYFEVSAGGYSTLERHEHTHAVLILRGRGTVRIGDETHSVGERDLVTIDPL
                     TWHQFLASAGEALGFLCLVSKERDRPQLPAGEVSTRQR"
     gene            complement(2391..>2645)
                     /gene="gltD"
                     /locus_tag="AC16HB_11465"
     CDS             complement(2391..>2645)
                     /gene="gltD"
                     /locus_tag="AC16HB_11465"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HIR5607454.1"
                     /translation="VLFAMGFVSPVAAVLDAFGVEKDPRGNARATTDGSGSYRTSVEK
                     VFAAGDMRRGQSLVVWAIREGRQCARAVDEYLMGTSTLPR"
BASE COUNT          440 a          908 c          848 g          449 t
ORIGIN      
        1 gccgttacgc ggtgctcgta gctccgctgc gggactcgag gcccaacggc ttgcccgttc
       61 cgcccgtccg gcggtagtcg atcccgatcg ctcttaccgc acagactgca gcgaatagat
      121 gtagtcgatc agcgacagga tgcgaacgcg gatgtagacc tccgggtcgt acggcgtgtc
      181 gatgtagtac ttgccggcct gcacgctgta atcctgcccc cagatcggca tttcgcgcgt
      241 tccatgcgac ttcagcgcct cacggccgtc gatgacttga tacaggcgat cggccgggaa
      301 cacgcccttg ttgcgctgac tgatcgtggt gatatcgggt atccgggtct tcagcagctc
      361 gaagtaggac ccatcgccct tgccggtcgt tccatggcag accgcgcagt tcgacatgta
      421 ttcgcgcttg ccgagttcgg tccgcaactg cccgagtgcc gagccggcgc aaacaagcgt
      481 cagcaccgcg accagcgcca ttgccatcct attccgtttc atattgcctc cactccacaa
      541 gccgattggc tcacgattcc actgacctct tcaggcgaag acttctgagt gctccgcccg
      601 ccacaacaac tatcaacgca attgcatgtt cgaccgacag gggcggcgcg cattgacccc
      661 tggcagttcg aatgccggtc acggtggcaa ccgtcgaaac gcctgccgaa cgcgcccacg
      721 tagaatcggc gcatgagcgc ggaccgcatt caagtcgtct accggctgca cgttccgccc
      781 gcgcaagcgc aggagcgcgc ccgactgctc gcgatcgagc aaagcctcga gatgcccccg
      841 gaagcggtac aggaaccgcg cgtgctcgac gaaattgcag cccgcgtcga gcgcatcgat
      901 ccgcaggtcg acggcacctc tcaggcgacg gtctcgctga gcgtcgacag catcggagac
      961 gatgccggcc agctgatgaa catgatgttc ggcaacagct cgctgcagcc ggatgtcgaa
     1021 gtactcgatg tcgacgttcc ggtgcagctg gcagcgatgt tcggcggccc caatctcggc
     1081 atcgaggggc tgcgtcgcct ggccggggcg cgcgatctcg cgctgacgtg cagcgcgctc
     1141 aagccgatcg gctcggggat cgcgcatctg tcgtcgctct gctcggcact ggcgcgcgca
     1201 ggaatcgacg tgatcaagga cgatcacggc tgggccaacc aggcaagtgc accgttcacc
     1261 cagcgcgtcg ccgggtgcca gcgcgcggtc gcggacgcca atgcgggtca atcagggcga
     1321 cagacgttgt atgcgccgag tctgtacggc acgcatgggc agatgcaacg acagatcgaa
     1381 ctggcgcgca agtacggcgt gaagatcgta ctgatcgcgc cgatggtatg cggcgtggcg
     1441 acgctgaacg cgctgcgacg cgagcacccg gacctcgcat tcctcgcgca ccccgcgctc
     1501 ggcggcagcg cgcgcattgc gccgccgaca ctgctcggca aactgtttcg cctgttcggc
     1561 gccgacgcgg tgatctttcc gaatcacggc ggccgcttca cctattcgcg cgcggtgtgc
     1621 accgcaattg cggcccacaa tcgcgccgaa tggcacggca tgcgccgcgc gctgcccacg
     1681 ccggccggcg gcatgagcct tgagcggtgc gcggagatcg tcgccgaata tggcacagac
     1741 acgatgctgc tgatcggcgg ctcgctgctg atcgcgcgcg agcacatcgg cgagcgcgcg
     1801 cgcgccttcg tggccgcggt tcgcgcggcg tcgatgcccg tcgcggcatg agcgcgatcc
     1861 ggcgcttcac tgccgatccg gccggcaact tcggctggca agatgtcgaa cggctgcgct
     1921 acaaggaaga aggctcggcg ccgtttcgtg acatcacgcg gcaggtgctg tttgcacagc
     1981 cggaccaggc gtgcgagctg cgctatttcg aagtctcggc gggcggctat tcgacgctcg
     2041 aacgccacga gcacacacac gcggtgctga tcctgcgcgg ccgcggcacg gtgcgcatcg
     2101 gcgacgagac gcattccgtc ggggagcgcg atttggtcac gatcgatccg ctcacctggc
     2161 accagtttct cgccagcgcc ggcgaggcgc tggggttcct gtgcctggtc agcaaggagc
     2221 gcgaccggcc gcagttgccg gcgggcgaag tctccacgcg gcagcgttga ctcccccatc
     2281 gggaatctgt cgatcgcgcg cggcaacgac cctcgctcgt cgtccgtctt cgcgcgcgtc
     2341 ccacgccttg ccgacgcgcc cgcttggcgg gcgccgggcg atccgcgact tcaccgcggg
     2401 agcgtcgaag tgcccatcag gtattcgtca accgcccttg cacactggcg gccttcgcgg
     2461 atcgcccaca ccacgagcga ctggccgcgt cgcatatcgc ctgcggcaaa cactttttcc
     2521 acgctggtcc ggtagctgcc gctgccgtcg gtcgtcgcgc gggcgtttcc gcgcggatcc
     2581 ttctcgacgc cgaatgcgtc gagcaccgcg gccaccggcg acacgaagcc catcgcgaac
     2641 agcac
//
LOCUS       DBNHBY010000465         2702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1788367, whole genome shotgun sequence.
ACCESSION   DBNHBY010000465 DBNHBY010000000
VERSION     DBNHBY010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1788367"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1808
                     /locus_tag="AC16HB_11470"
     CDS             267..1808
                     /locus_tag="AC16HB_11470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5607455.1"
                     /translation="MQIAGVDFAALPGAIAAGVATVDDSQWVDIAKTCARDGIRLVAL
                     WGSDRRDLGDGLVVSALYAGRDALACVRLPVSGTPLEYPDIAARFPAAGRMQRATYDL
                     LGLRARGAGDERPWLRHGAWPADYFPLRFDADGARQFEVEQERYAFVPVAGDGVHEIA
                     VGPIHAGIIEPGHFRFSVVGEKVLRLEERLGYVHKGIEKRFAGMPLTDGYRLAGRVSG
                     DSTVAFSWAYAMAAESATGCTIPPRAAWLRALLLERERIANHLGDLGALGNDAALAFG
                     LAQFSRLREQWLRANAEAFGHRLLMDCIAPGGVARDPAAEALGALRRQCDAFERELRE
                     LRGIYDDHAGLQDRFRNAGVVTPELARLLGLAGLAARASGQPRDVRIDRPCAPYDELG
                     VGLACETAGDVAARVAVRFDETFESLRLIRAIVERLPSGEVRCDLPATGKQGFGLGWV
                     EGWRGDVLVALESDADARLRRCHCHDPSWQNWPLLEHAVIGNIVPDFPLINKSFNLSY
                     SGQDL"
     gene            1808..2332
                     /locus_tag="AC16HB_11475"
     CDS             1808..2332
                     /locus_tag="AC16HB_11475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5607456.1"
                     /translation="MLILLRQIARSGICTEPAPTPQDAWRVEGERMQGEVLRLLGRAL
                     TIRQIDAGSCNGCELEIHALNNPYYNIEGRGIRFVASPRHADLLLVTGPVARNMQIAV
                     RRAYDATPEPKLVVAVGDCGCTGGIFGESYASCGRVANVIPVDVAIPGCPPPPLEILR
                     GVLTAVAQRATAHG"
     gene            2543..2702
                     /locus_tag="AC16HB_11480"
                     /pseudo
     CDS             2543..2702
                     /locus_tag="AC16HB_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
BASE COUNT          376 a          923 c          960 g          443 t
ORIGIN      
        1 gtccgtgagt aattcgtacg atgcgttgcc accagaaagc ccggatgcaa ggcgcgcggc
       61 gcaggacagt agccgggcct acggccaagc cgcgcaacac cgcagccggg ctttctggcg
      121 gcaacccttc gggacgggcc gatttggctc gcattgcgtt gttgctcgtc gctcatttgg
      181 attgaccaaa ctgcgctcct cgcgcctagc actgcaagcc aaatcggccc gtcgcacgca
      241 cgaattgctc acggacaagc tctaagatgc agatcgccgg cgtcgatttc gcagcgctgc
      301 ccggtgcgat cgcggcgggc gtcgccacgg tcgatgactc gcagtgggtg gatatcgcga
      361 agacctgcgc ccgcgatggc atccggctgg tcgcgctgtg gggttcggat cggcgcgacc
      421 tcggtgacgg cctggtcgtc agcgctttgt atgcagggcg ggacgcactc gcgtgcgtgc
      481 ggctgccggt atccggcacg ccgctcgaat atccggacat cgccgcgcgg tttccggccg
      541 ccggccggat gcagcgtgcg acgtacgatc tgctcgggct gcgcgcccgc ggcgccgggg
      601 acgagcgtcc ctggctgcgc catggcgcct ggcccgccga ctactttccc ttgcggttcg
      661 atgccgacgg cgcgcggcag ttcgaggtcg agcaggagcg ctacgcgttc gtccctgtcg
      721 ccggcgacgg cgtgcacgag atcgccgtcg gcccgattca tgccggcatc atcgagccgg
      781 gacacttccg cttctcggtg gttggcgaga aggtgctgcg tctggaggag cgcctcggct
      841 atgtccacaa gggcatcgag aagcgattcg cgggcatgcc gctgacggac ggataccggc
      901 tcgccggacg tgtatcgggc gactccaccg tcgctttctc ctgggcgtac gcgatggcag
      961 ccgaatccgc gacgggctgc acgatcccgc cgcgcgcggc ctggttgcgc gcattgctcc
     1021 tggaacgcga gcgcatcgcc aaccacctcg gcgatctcgg cgcgctcggc aacgatgcgg
     1081 cgctggcgtt cgggctggcg cagttctcgc ggctgcgcga acagtggctg cgcgcgaacg
     1141 cggaagcgtt cggccaccgg ctgctgatgg actgcatcgc tcccggcggc gtggcgcgcg
     1201 atccagccgc cgaagcactc ggcgcgctgc gccggcaatg cgatgccttc gagcgcgagt
     1261 tgcgcgaact gcgcgggatc tacgacgatc atgccggcct gcaggaccga ttccgcaacg
     1321 cgggcgtcgt cacgcccgaa cttgcccggc tgctcggatt ggcggggctc gccgcacgcg
     1381 ccagcggtca gccgcgcgac gtgcgcatcg atcgcccgtg tgcgccgtac gacgagctcg
     1441 gcgtgggcct ggcctgcgag acggccggcg atgtcgccgc gcgcgtcgcg gtgcgtttcg
     1501 acgaaacctt cgagtcgctg cggctgatcc gcgccatcgt cgagcggctg cccagcggcg
     1561 aagtgcgttg cgacctgccg gcgaccggga agcagggctt cgggctcggc tgggtcgaag
     1621 gctggcgcgg cgacgtgctg gtcgcgttgg aatccgacgc cgacgcgcgg ctgcgccgtt
     1681 gccactgcca cgatccgtcg tggcagaact ggccgctgct ggagcacgcc gtgatcggca
     1741 acatcgttcc cgactttccg ctgatcaaca agtcgttcaa cctcagctac tcgggacagg
     1801 atctctgatg ctgattcttc tccggcagat cgcgcgctcc ggcatctgta ccgagcctgc
     1861 gcccacaccg caggatgcct ggcgggtcga aggcgaacgg atgcagggtg aagtgctgcg
     1921 gttgctgggc cgcgctctga ccatccgcca gatcgacgcc ggttcctgca acggctgcga
     1981 actggagatt cacgcgctga acaatcccta ctacaacatc gaagggcgag gcatccgatt
     2041 cgtcgccagc ccgcgccacg cggacctgct gctggtgacg gggcccgtgg cgcgcaacat
     2101 gcagatcgcg gtcaggcgcg cgtatgacgc cacgccggag cctaagcttg tcgtcgcagt
     2161 gggcgactgc ggctgcacgg gcggcatctt cggcgaaagc tatgcgagct gcgggcgcgt
     2221 cgccaacgtg attcccgtcg atgtggcgat ccccggctgc ccgccgccac cgctcgagat
     2281 cctgcgcggc gtgttgacgg cggtcgcgca gcgcgcgacc gcacacgggt gagatggaag
     2341 atcaaggcga ggccgtgcag tgctagacat cctgctgtgg tgcatcgtgg cgttgctggt
     2401 ggccgccccc gtcgggctgg ccgcggcacg cgtcacggcc acacgggcgc tcgtgtatgc
     2461 gccggcgctg gttgcgagcg gcgtggcact ggccgcggcg gcgagcgcca tgctggcgcc
     2521 gggcaacggc gtgcagacac tgcgcctgcc gctcggcccg caccggctcg ccggcctcgt
     2581 gctgatcctg gcgctcgtcg gcgcgggctc gaaggcgggc ctcgcgccgc tgcacgcctg
     2641 gctgccgctc gcccatcccg ccgccccgag ccacgtctcg gcgctgatga gcggcgtgat
     2701 ga
//
LOCUS       DBNHBY010000466        15251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_774855, whole genome shotgun sequence.
ACCESSION   DBNHBY010000466 DBNHBY010000000
VERSION     DBNHBY010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_774855"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="AC16HB_11485"
     CDS             <1..145
                     /locus_tag="AC16HB_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR5607457.1"
                     /translation="TQIAEELALPPVKIHCSILAEDAIKAAINDYRKKHGLDQPAEQQ
                     KAA"
     gene            177..500
                     /gene="iscA"
                     /locus_tag="AC16HB_11490"
     CDS             177..500
                     /gene="iscA"
                     /locus_tag="AC16HB_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HIR5607458.1"
                     /translation="MAVTMTERAASHVAGFIAKRGKGLGLRLGVRTSGCSGMAYKLEY
                     ADTVNPEDEQFESHGVRIFVDPKSLAYLAGTELDFVREGLNQGFKFNNPREKDRCGCG
                     ESFRV"
     gene            589..1134
                     /gene="hscB"
                     /locus_tag="AC16HB_11495"
     CDS             589..1134
                     /gene="hscB"
                     /locus_tag="AC16HB_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIR5607459.1"
                     /translation="MASNIPDHFALFGLERRFAVDLDALDAAYKHVQDKVHPDRFAAA
                     APTERRIAMQWAARANEALQTLRTPARRAAYLCELNGVAPGTESNTAMPPAFLMQQLE
                     WREALGDAAAAGDSRALDMLARQTEAARAELIDDLARAIDTERDFVRASALMRQLMFA
                     DKMRDEIDHARHAQTAPSSAG"
     gene            1140..3008
                     /gene="hscA"
                     /locus_tag="AC16HB_11500"
     CDS             1140..3008
                     /gene="hscA"
                     /locus_tag="AC16HB_11500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR5607460.1"
                     /translation="MALLQIAEPGMSTAPHEHRLAAGIDLGTTNSLVATVRHGVSEVI
                     ADEQGRPLLPSVVRYLADGRVEVGFEAQRHQATDPRNTIVSVKRFMGRGVKDVEHVEN
                     LPYDFVDAPGMLQIKTAAGVKSPVEVSSEILKALRLRAEAALGGELVGAVVTVPAYFD
                     DAQRQATRDAARLAGLNVLRLLNEPTAAALAYGLDNAAEGIYAVYDLGGGTFDISILR
                     LTKGVFEVLSTGGDTALGGDDFDHRLYCWALEQASLSLLEPEDQRLLTVKARSAKETL
                     STRASTTMQARLGNGHWINLSISADSFAAMTQHLVAKTLDATRRALRDAKLKIADVKG
                     VVMVGGATRMPHVRKAVGELFETEPLTNIDPDQVVAIGAALQANLLAGNHLSDDWLLL
                     DVIPLSLGLETMGGLVEKIIPRNSTIPIARAQEFTTFRDGQTAMAIHVVQGERELVSD
                     CRSLARFELRGIPPMVAGAARIRVTFEVDADGLLSVSARETTSGVEASVTVKPSYGLG
                     DEDIARMLRESMDAAGTDVALRMLREQQVEAERLLAATQAALAADGDLLTVEERALID
                     ARAAQLAAVERGDDPDAIKRAISALAEATDGFAARRMDRSIRAALAGRKVEDIAVK"
     gene            3011..3346
                     /gene="fdx"
                     /locus_tag="AC16HB_11505"
     CDS             3011..3346
                     /gene="fdx"
                     /locus_tag="AC16HB_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HIR5607461.1"
                     /translation="MPKITILPHAEICPEGTEIEAKKGVSICRNLLDQDIEIEHACEL
                     SCACTTCHVIVRQGFETLNPSDEDEDDLLDKAWGLTPQSRLSCQAIVDDADLVIEIPK
                     YSINQIKEG"
     gene            3352..3546
                     /gene="iscX"
                     /locus_tag="AC16HB_11510"
     CDS             3352..3546
                     /gene="iscX"
                     /locus_tag="AC16HB_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR5607462.1"
                     /translation="MKWTESLEIAIALAEKHPDVDPRYVNFVDLYHWVLALPGFNDDP
                     KHSGEKILEAIQQAWIDEAE"
     gene            complement(3550..3852)
                     /locus_tag="AC16HB_11515"
     CDS             complement(3550..3852)
                     /locus_tag="AC16HB_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5607463.1"
                     /translation="MQTHAKKLLVVITEAALEKALIRDARAFGAHGYTIGDVRGGGER
                     GERAAAWEADRSIEMKVICDEDVATRLAAHVLDTYVPNYAVTLFVAEVGVFRPQKF"
     gene            complement(3855..4844)
                     /locus_tag="AC16HB_11520"
     CDS             complement(3855..4844)
                     /locus_tag="AC16HB_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HIR5607464.1"
                     /translation="MHARIRAGRSRRVAAEGRPIDPVVLFFVLGVIARLAKSDLRLPE
                     ALYEALSIYLLLAIGLKGGVELAQQPVAAVLPQALAAIALGAAIPFLLFPVLLKLGRL
                     PRADSASIAAHYGSVSVVTFAVASSFLLQRQVAAESYLPLLVALMEAPGIVSGIVLAR
                     IGRGQRIAWGALAHEIGFGKSVVLLLGGLAIGAIAGPSGAKPIEPLFMGLFKGVLALF
                     LLEMGLVAGARLAELKRAGAFLVLFGLVAPVVLGTLGAVVGHLIGLSIGGTALLATLA
                     ASASYIAAPTAMRIAVPEANPALSIGVALGVTFPFNITLGIPLYLQLARWIGG"
     gene            complement(4784..5587)
                     /locus_tag="AC16HB_11525"
     CDS             complement(4784..5587)
                     /locus_tag="AC16HB_11525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5607465.1"
                     /translation="MSRERLRALQAKLLARARQPAPDGWLPVVMAQAQVGVAHPDVAG
                     FLATHEPRFLLLDYRLVLDDADLDVRGRTALLFDAAVRLRDAGLLRGWRDEALDIRPA
                     PDTAPIATIERAACRTLGITTTAVHLNALTATGDMVVARRADHKQIDPGLWDNLVGGM
                     VPAGESELDALAREALEEAGLDLSALAVVRGGRVHVTRVVPEGYQSEIVQVFDTVLPA
                     HAQPHNIDGEVAAIEVRPVNAVVAAIERDEFTLESALVALDALLRRADR"
     gene            complement(5584..7092)
                     /gene="lysS"
                     /locus_tag="AC16HB_11530"
     CDS             complement(5584..7092)
                     /gene="lysS"
                     /locus_tag="AC16HB_11530"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR5607466.1"
                     /translation="MTDTTPPPFDENQIIGERRAKLARLRTAGPAFPNDFARTHHAGE
                     LHAKYGSVEREALAKEKIEVALAGRMMLKRVMGKASFATIQDTSSRIQFYIADDDTGR
                     DAHEAFKGWDIGDIVAARGELFKTQKGELSVRCRQIRLLAKALRPLPDKFHGLEDRER
                     RYRQRYVDLMMNEATRSAFVTRSKAIAAMRAFMVDNGFLEVETPMLQVIPGGAAAKPF
                     VTHHNALGIDMFLRIAPELFLKRLVVGGFERVFEINRNFRNEGLSPRHNPEFTMMEFY
                     AAYHEYRWLMNFTEELLRETAIRATGSALLNYQGHAIDLSMPFARLTIVEAIRAHGPQ
                     YTDAQLTDAQYLHAELARLGSTHSKEAGLGALQLALFEEVAESKLIQPTYIVDYPVEV
                     SPLARASDIRPGITERFELFITGREMANGFSELNDPEDQAARFTEQAKAKEAGDEEAM
                     YYDADYVRALEYGMPPAAGCGVGVDRFVMLLTDAPSIRDVILFPHMRPEERG"
     gene            complement(7106..7864)
                     /locus_tag="AC16HB_11535"
     CDS             complement(7106..7864)
                     /locus_tag="AC16HB_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5607467.1"
                     /translation="MSVDLYIITGASRGLGRALAQQLMERDRTLLTIARHPDRSLDEV
                     ATAAGAHLTQWALDLAHDVGVAARLEAWLHQQASRRHATATLINNAGLLGRIGPIEAM
                     EADELAAVLRVDLEAALVLTASFLRATRHWQAKRRVLNISSGAGRLVLAGCAAYCAAK
                     AGLDHFARVAAADEKLQPNPAQIVSLGPGVIDTDMQAALRGSDASGFPDLPRFVELKE
                     SGQLVDVNAAAARCLAYLSRPDFGANPVADVRDA"
     gene            complement(7861..8965)
                     /gene="prfB"
                     /locus_tag="AC16HB_11540"
     CDS             complement(join(7861..8883,8885..8965))
                     /gene="prfB"
                     /locus_tag="AC16HB_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588915.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5607468.1"
                     /translation="MEPERLNQLSASITDLNARAAELRRYLDVDAKAHRLSQVARELE
                     NPNVWDNPKQAQELGKEKKLLDDVVSRMERLTGGLRDTQELFEMARADNDDATLISIE
                     VDAGSLGRQVAELEFQRMFNNPLDPNNCFIEIQAGAGGTEAQDWASMLERMYLRYAER
                     KGFKAELLDETPGEVAGIKGATIKLSGAYAYGTLRTETGIHRLVRKSPFDSNARRHTS
                     FASVFVYPEVDETIEIEINPADLRIDVYRASGAGGQHVNKTESAVRITHLPSGIVVQS
                     QNDRSQHRNRAECMAMLKSRLYEQELRKRMVEQTKLEEGKTDIGWGHQIRSYVLDQSR
                     IKDLRTNIEVGNTQSVLDGDLDQFIEASLKQGV"
     gene            complement(9137..10831)
                     /gene="recJ"
                     /locus_tag="AC16HB_11545"
     CDS             complement(9137..10831)
                     /gene="recJ"
                     /locus_tag="AC16HB_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5607469.1"
                     /translation="MSAVRFVPRVFDAAAAQRLADAGLPRPLARALAARGVGSLQDLT
                     LVTRDLIAPPQLAHVEDAARLLADAIDAQRSMTIIADYDCDGATACAVGVRGLRALGA
                     RVDYLVPNRFEYGYGLTPEIVTLASQRRPDLLITVDNGIASVDGVARARELGIDVLIT
                     DHHLPGPQLPAAAAIVNPNQPGCGFPSKHLAGVGVMFYVLLALRAELRARGRFTESTQ
                     PRLDALLDLVALGTVADVVRLDHNNRILVAAGLERIRSERMQPGIAALFQVAGRAAQL
                     ARPSDLGFALGPRINAAGRLADMSTGIECLLADDFERALALARQLDELNRERRAIEVD
                     MQFDALADLQDASFAERRTITLFNDGWHQGVVGLVASRVKERFHRPTVACARVGEATL
                     RGSGRSIEGVHLRDTLDLVTKLAPGLVEKFGGHAMAAGLTLRAEHYEGFASAFEEATR
                     ATADATLFRRTVDTDGPLVADEISLGLVEAIDRQIWGQGFPPPLYDNEFAIVEQRLIK
                     EQHLKLTVELQGRRFGAMWFRHTDTLPSRARLAYRPAADDYQGQRRVTLFIEHALA"
     gene            complement(10828..11895)
                     /locus_tag="AC16HB_11550"
     CDS             complement(10828..11895)
                     /locus_tag="AC16HB_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607470.1"
                     /translation="MRWFVLVPGSLVPAPLAADIVGAAHAEWIADCLVRAQRAPDESV
                     RDAPAGAVHLAWLWRAFCGDGVIPVTAPYAWRAANHASALLPDDSSQWWHCEPIHIDV
                     GRDQLLIGRSADAQLTDAEADALAAEADPVLREHGGLLKRLRHDAWFMQTDRPWRLDT
                     APLASARGQPLGPCLPVGPDAGRWRKVLTEIQVRWHHHAVNTAREQRGLQPVNGVWLH
                     GGGVWAPLPRAPFASIVSADATLRGWALAAGLPPASLYDEDDLPSQPGDAVSLWTDLA
                     EPAAFDAWGVWIERLGAFEQRLERLRDRAWGRGFNSIELVLTGRQFLRVLSLRRHDRL
                     RFWRRPSGGALAALLGEPVAQ"
     gene            11958..13214
                     /locus_tag="AC16HB_11555"
     CDS             11958..13214
                     /locus_tag="AC16HB_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR5607471.1"
                     /translation="MNLPYEVRIGLRYTRAGRRARRRNGFISFISAISMGGIMLGVAA
                     LIVVLSVMNGFTKEVRDRMLSVVSHVELSSFSGALGDWRQVAQRARTNPEVIAAAPYV
                     NAQAMLTRDDVVRGALIRGIDPSVEGGVSDIVRNLPSGKIMDLAEGEFGIVPGIELAR
                     ALGVSVGDKITLLAPQGNVTPAGVVPRLKQFRVVGLFESGHYEFDSTLALVHIADAEK
                     MFRVSGPTGVRLKLKDALEAPQVAEQLAHGFDADMQVRDWSRQNRTYFAAVKTEKRMM
                     FIILTLIVAVAAFNLVSTLVMTVTEKQSDIAILRTLGASPRSVMSVFIVQGALIGVFG
                     TLGGVGLGLLLANNLDVIVPAIEHAFGVQFLPKDIYFLNSLPSDPRAGDIVPIAIISL
                     VLSLVATLYPSWRAARTRPAEALRYD"
     gene            13207..13884
                     /locus_tag="AC16HB_11560"
     CDS             13207..13884
                     /locus_tag="AC16HB_11560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607472.1"
                     /translation="MTEVIAARGIFKAYREDRLVVEVLKGADLEVRRGEVIAIVGASG
                     SGKSTLLHCLGGLDAVDRGAVRIGGRDVAALGERERGDLRNRALGFVYQFHHLLPEFT
                     ALDNVAMPLSIRRLPLETAREQAAAALGAVGLGERLQHHPAELSGGERQRTAIARAIV
                     TKPACVLADEPTGNLDRETARGVFDVFLALARSSGTAIVIVTHDPELAAQCDRTMRLR
                     QGVLSSA"
     gene            complement(13887..14237)
                     /locus_tag="AC16HB_11565"
     CDS             complement(13887..14237)
                     /locus_tag="AC16HB_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607473.1"
                     /translation="MPILHRAWPLLLAALLGYLFDLEAIGFGALLGAGAFVALLLARR
                     PMPFKFGALALAGAVSFGCIAAVLAALHGMPVREMMASGEAAVLLPKILGVAAVMLAV
                     LGVACLLWGSIFDS"
     gene            complement(14237..14794)
                     /locus_tag="AC16HB_11570"
     CDS             complement(14237..14794)
                     /locus_tag="AC16HB_11570"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR5607474.1"
                     /translation="MLRRFFKRYMPSADKVREVRAFQLFGERIFHPALWHLNRRTAAG
                     GVAIGLFCGLIPGPFQMIGAGIAALTLRMNLPIALLTTLYTNPLTIVPLYFIAYQIGS
                     FALGAASGMEPEPPPDWVWTQPAASAEAFSQWMIGLGAPLALGVLLLASLLSLSGYLI
                     VRVIWNLHLRHAWHARRKRRAGSGV"
     gene            14890..>15251
                     /locus_tag="AC16HB_11575"
     CDS             14890..>15251
                     /locus_tag="AC16HB_11575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5607475.1"
                     /translation="MWLDTHCHLDVPEFDADRDAVVARARQAGVSMMVIPAVEVAAFA
                     RVKECAHCCGYAYALGIHPLYVERAEEDDIGRLRAAVEEALADPRFVAIGEIGLDNYV
                     ATPPRERQQWFYAEQLKIA"
BASE COUNT         2440 a         5172 c         5258 g         2381 t
ORIGIN      
        1 cacgcagatc gccgaggaac tggcgctgcc gccggtcaag attcactgct cgatcctggc
       61 cgaggacgcg atcaaggcgg cgatcaacga ttaccgcaag aagcacggtc tcgaccagcc
      121 cgcggaacag cagaaggcag cctgaacgcc acgtcggtag aagagggagt caagctatgg
      181 ccgtcacgat gacggagcgc gcggcaagcc acgttgccgg cttcattgcc aagcgcggca
      241 agggcctcgg cctgcgcctc ggagtacgca ccagcggttg ttctgggatg gcatacaagc
      301 tcgagtacgc ggacaccgtg aatcccgagg acgagcagtt cgagagccac ggcgtgcgca
      361 tcttcgtgga tccgaagagc ctggcgtatc tggccggcac ggagctcgac ttcgtgcgcg
      421 aaggactcaa ccagggattc aagttcaaca acccgaggga aaaggaccgc tgcggctgtg
      481 gcgagtcgtt ccgggtctga gcggcgcggc gccgccgttc cgagtctcac ccgatcacgc
      541 ggctgtccga cggcaagtcg acccggcagc cgctttttca tttccgccat ggcgagcaac
      601 atcccggatc acttcgcgct cttcggtctc gagcgtcgct tcgcggtcga cctcgacgcg
      661 ctcgacgccg cctacaagca cgtgcaggac aaggtgcacc ccgatcgttt cgccgcggca
      721 gcgccaacgg agcggcgtat cgcgatgcaa tgggcggcgc gcgccaacga ggcgctgcag
      781 acgctgcgga cgcccgcgcg gcgcgcagcg tacttgtgcg agctcaacgg cgtggcgccc
      841 gggaccgagt cgaacacggc gatgccaccg gcgttcctga tgcagcagct cgagtggcgc
      901 gaggcgctcg gggacgcggc ggccgcgggg gattcgcggg cgctcgatat gctggcgcgg
      961 cagacggagg cggcgcgcgc cgagctgatc gacgatcttg cgcgggcgat cgacaccgag
     1021 cgtgatttcg ttcgtgcctc ggcgctcatg cgccagctga tgttcgccga caagatgcgc
     1081 gacgagatcg accacgcgcg ccacgcccag acggctccat ccagcgccgg ctagatcgca
     1141 tggcactgct gcagattgcc gaacccggca tgtcgaccgc gccgcacgag catcggctcg
     1201 cggccggcat cgatctcggc acgacgaact cgctggtcgc cacggtccgg cacggcgtga
     1261 gcgaggtcat cgccgacgag cagggccggc cgctgctgcc gtcagtggtt cgctatctgg
     1321 ccgatggccg cgtcgaagtc ggcttcgaag cgcagcggca tcaggcgacc gacccgcgca
     1381 acacgatcgt ctcggtcaag cggttcatgg ggcgcggcgt caaggacgtc gagcacgtcg
     1441 agaacctgcc gtacgacttc gtggatgcgc cggggatgct gcagatcaag acggcggccg
     1501 gagtcaagag cccggtcgag gtctcgtcgg aaatcctgaa ggcgcttcgg ctgcgggccg
     1561 aagctgcgct cggcggcgaa ctcgtcggcg cggtcgtgac cgtgccggcg tacttcgacg
     1621 acgcgcagcg ccaggcgacg cgcgatgccg cgcgtcttgc gggactcaac gtgctgcggc
     1681 ttctgaacga accgaccgcg gcggcgctgg cctacggcct cgacaacgcg gccgaaggca
     1741 tctacgccgt ttatgacctc ggcggcggca cgttcgacat ctcgatcctg cggctgacca
     1801 agggcgtgtt cgaggtgctg tcgactggtg gcgatacggc gctcggcggc gacgacttcg
     1861 atcatcggct gtactgctgg gctctcgagc aggccagcct gtcgctgctc gagcccgaag
     1921 accagcggct gctcaccgtc aaggcgcgca gcgcgaagga gacgctgtcg acgcgggcga
     1981 gcacgacgat gcaggcgagg ctggggaacg gtcactggat caacctgtcc atcagcgccg
     2041 acagctttgc cgcgatgacg cagcatctgg tcgccaagac gctcgacgcc actcggcgtg
     2101 cgctgcgcga tgcgaagctg aagattgccg atgtgaaagg cgtggtgatg gtcggcggcg
     2161 ccacgcgcat gccgcacgtg cgcaaggccg taggagagct gttcgagaca gaaccgctca
     2221 ccaacatcga ccccgaccag gtggtggcga tcggtgccgc gctgcaggcc aacctgctcg
     2281 ccggtaatca cctcagcgac gactggctgc tgctcgacgt gatcccgctg tcgctcgggc
     2341 tcgaaacgat gggcgggctg gtcgagaaga tcatcccgcg caattcgacg atcccgatcg
     2401 cgcgcgcgca ggagttcacc actttccgcg acggccagac ggcgatggcg attcacgtgg
     2461 tgcagggcga gcgtgaactg gtcagcgact gccgctcgct cgcgcgcttc gagctgcgcg
     2521 gcatcccgcc gatggtggcg ggcgcggcgc gcatccgtgt cacgttcgag gtcgatgccg
     2581 acgggctgct gtcggtgtcg gcgcgcgaga cgacaagcgg cgtcgaggcg tcggtgacgg
     2641 tcaagccgtc ctatggcctc ggggacgagg acatcgcgcg catgctgcgc gaatcgatgg
     2701 acgccgcagg taccgacgtc gcgctgcgca tgctgcgcga gcagcaggtc gaggccgagc
     2761 gcttgctggc ggccacgcag gcggcgctcg ccgccgacgg cgatctgctg acggtcgagg
     2821 agcgcgcgct gatcgatgcg cgcgcggcgc agctcgctgc ggtcgagcgc ggcgacgacc
     2881 cggacgcgat caagcgcgcg atttcggcgc ttgccgaggc gaccgacggc ttcgcggcgc
     2941 gccgcatgga ccgctcgatc cgcgccgcgc tggcggggcg caaggtcgag gacattgcgg
     3001 tcaagtagcg atgccgaaga tcaccatcct gccgcacgcg gagatctgcc ccgagggcac
     3061 cgagatcgag gcgaagaaag gcgtgagcat ctgccgcaat ctgctcgacc aggacatcga
     3121 gatcgagcat gcgtgcgagc tgtcgtgcgc ctgcacgacc tgtcacgtga tcgtgcgcca
     3181 gggattcgaa acgctcaatc cgtcggacga ggacgaggac gatctgctcg acaaggcgtg
     3241 gggtctgacg ccgcagtcgc gtctgtcgtg ccaggcgatc gtcgacgacg cggacctggt
     3301 gatcgagatc ccgaagtact cgatcaacca gatcaaggag gggtgacact gatgaagtgg
     3361 actgaatcgc tcgagatcgc gatcgcactg gccgagaaac atcccgacgt cgacccgcgc
     3421 tacgtgaact tcgtcgacct gtaccactgg gtgctcgcgc tgcccggctt caacgacgac
     3481 cccaagcact cgggcgagaa gatcctcgag gcgatccagc aggcgtggat cgacgaagcc
     3541 gagtagcgct cagaacttct gcgggcggaa caccccgacc tcggcgacaa acagcgtgac
     3601 cgcgtagttc ggcacgtagg tgtcgagcac gtgcgcggcc agccgcgtgg cgacgtcctc
     3661 gtcacagatc accttcatct cgatcgagcg gtcggcctcc catgccgcgg cgcgctcgcc
     3721 gcgctcgccg ccgccgcgca cgtcgccgat cgtgtagccg tgcgcgccga acgcgcgcgc
     3781 gtcgcggatc aacgccttct ccagcgcggc ctcggtgatc accaccagga gcttcttcgc
     3841 gtgcgtctgc atggtcagcc tccgatccag cgcgccagct gcaggtacag cggaatcccg
     3901 agcgttatgt tgaaggggaa cgtgacgccg agcgccacgc cgatcgacag cgcggggttg
     3961 gcctcgggca ccgcgatgcg catcgccgtc ggtgcggcaa tgtacgacgc gctcgccgcc
     4021 agcgtcgcca gcagcgcggt gccgccgatc gacagcccga tcagatggcc gacgacggcg
     4081 ccgagcgtcc ccagcacgac cggcgcgacg aggccgaaca gcacgaggaa cgcgccggcc
     4141 cgcttcagtt ccgccagccg tgcgccggcg accagcccca tttcgagcag gaacagcgcc
     4201 agcacgccct tgaacaggcc catgaagagc ggctcgatcg gcttggcgcc gcttggaccc
     4261 gcgatggcgc cgatcgcgag gccgcccaga agcagcacga cgctcttgcc gaagccgatc
     4321 tcgtgcgcca gcgcgcccca ggcgatccgc tgcccgcgtc cgatgcgcgc gagcacgatg
     4381 ccggacacga tgcccggcgc ctccatcagc gcgaccagaa gcggcagata gctttccgcc
     4441 gccacctgcc gctgcagcag gaagctcgac gccaccgcga acgtcactac cgacaccgag
     4501 ccgtagtgcg cggcgatcga tgcgctgtcg gcgcgcggca agcggccgag cttcagcagc
     4561 accgggaaca gcaggaacgg aatcgctgcg ccgagggcga tcgccgccag cgcctgcggc
     4621 agcacggccg cgaccggctg ctgcgccagc tcgacaccgc ccttgagtcc gatcgccagc
     4681 agcaggtaga tcgacagcgc ctcgtacagc gcctccggca aacgcaggtc gcttttcgcc
     4741 aggcgcgcga tcacgccgag cacgaagaac aggacgaccg gatctatcgg tcggccctcc
     4801 gcagcaacgc gtcgagagcg accagcgcgg attcgagcgt gaattcgtcg cgctcgattg
     4861 cggcgaccac ggcgttgacc ggccgcacct cgatcgccgc cacctcgccg tcgatgttgt
     4921 ggggctgcgc gtgggccggc agcaccgtgt cgaacacctg cacgatctcg gactggtagc
     4981 cctccggcac cacgcgggtg acgtgcacgc gcccgccgcg cacgacggcg agcgcggaca
     5041 gatcgagtcc ggcttcctcg agcgcctcgc gcgccagcgc atcgagctcg ctctcgccgg
     5101 ccggcaccat gccgccgacg aggttgtccc acagtccggg atcgatctgc ttgtgatcgg
     5161 cgcgccgcgc cacgaccata tcgccggtcg cggtcaacgc gttcaggtgc accgcggtcg
     5221 tcgtgatgcc gagcgtgcgg caggccgcgc gctcgatcgt cgcgatcggt gccgtgtcgg
     5281 gcgcgggccg gatgtcgagc gcctcgtcgc gccagccgcg cagcagtccg gcatcgcgca
     5341 gccgcacggc cgcgtcgaag agcagggcgg tgcgcccgcg cacgtcgaga tcggcgtcgt
     5401 cgagcacgag acggtagtcg agcagcagga agcgcggttc gtgcgtggcg agaaagccgg
     5461 cgacgtccgg gtgcgcgaca ccaacctgcg cttgcgccat cacgacgggc agccagccgt
     5521 ccggcgccgg ttgccgtgcg cgcgccaaca gctttgcctg caacgcacgc agccgctcgc
     5581 ggctcaaccg cgttcctccg ggcgcatgtg cggaaacagg atcacgtcgc ggatgctcgg
     5641 cgcgtcggtc agcagcatca cgaaccggtc gacgccgacg ccgcagcctg cggccggcgg
     5701 catcccgtac tcgagcgcgc gcacgtagtc ggcgtcgtag tacatcgctt cctcgtcgcc
     5761 ggcttccttc gccttcgcct gctcggtgaa gcgcgcggcc tggtcttccg gatcattgag
     5821 ttccgagaag ccgttggcca tttcgcgccc ggtgatgaag agttcgaagc gctcggtgat
     5881 gccaggccgg atgtcggagg cgcgcgccag cggcgacacc tcgaccgggt agtcgacgat
     5941 gtaggtcggc tggatcagct tcgattcggc gacttcctcg aacagcgcga gttgcagcgc
     6001 gccgagcccc gcttccttcg aatgcgtgct gccgagccgc gccagttccg cgtgcaagta
     6061 ctgcgcgtcg gtgagctgtg cgtcggtgta ttgcggcccg tgcgccctga tcgcttcgac
     6121 gatcgtcagg cgggcgaacg gcatcgaaag gtcgatcgcg tggccctggt agttcagcaa
     6181 cgccgagccg gtggcgcgga tcgcggtctc gcgcagcagt tcctcggtga agttcatcag
     6241 ccagcggtac tcgtggtaag ccgcatagaa ctccatcatc gtgaactcgg ggttatgccg
     6301 cggtgacagg ccctcgttac ggaagttgcg gttgatctcg aacacccgtt cgaacccgcc
     6361 gaccaccagc cgcttcagga acagctccgg cgcgatgcgc agaaacatgt cgatgcccag
     6421 cgcgttgtgg tgcgtgacga agggcttggc cgcggcgccg cccgggatca cctgcagcat
     6481 cggtgtctcg acctcgagga agccgttgtc gaccatgaag gcgcgcattg ccgcgatcgc
     6541 cttgctgcgg gtgacgaacg cgctgcgcgt ggcttcgttc atcatcaggt cgacgtagcg
     6601 ctggcgatag cggcgctccc ggtcctccaa tccatggaac ttgtccggca gcgggcgcag
     6661 cgctttcgcc agcagccgga tctgccggca gcgcaccgac agctcgccct tctgcgtctt
     6721 gaacaactcg ccgcgcgcgg cgacgatgtc gccgatatcc cagcccttga acgcctcgtg
     6781 cgcatcgcgt ccggtgtcgt cgtcggcgat gtagaactgg atccgactgg aggtgtcctg
     6841 gatcgtcgcg aagctcgcct tgcccatcac gcgcttgagc atcatgcggc cagccagcgc
     6901 gacctcgatc ttctctttcg ccagcgcctc gcgctcgaca ctgccgtact tggcgtgcag
     6961 ttcgccggca tggtgcgtgc gtgcgaagtc gttcggaaac gccgggccgg cggtgcgcag
     7021 acgggcgagc ttggcgcgcc gctcgccgat gatctggttc tcgtcgaacg gcggcggggt
     7081 cgtgtcggtc atcgggttct ttttctcagg cgtcgcgcac gtcagcgacc gggttggcgc
     7141 cgaagtcggg gcgcgacagg tacgcgaggc agcgcgcggc ggcggcgttg acgtccacta
     7201 gctgaccgct ctccttcagt tcgacgaagc gcggcaggtc tggaaagccg ctcgcgtccg
     7261 acccgcgcag cgcggcctgc atgtcggtgt cgatcactcc gggccccagc gagacgatct
     7321 gcgctggatt cggctgcagc ttctcgtccg cagcggcgac gcgcgcgaaa tgatcgaggc
     7381 cggccttcgc cgcgcagtac gcggcacagc cggcgagcac gagccgcccg gcaccggacg
     7441 agatattcag cacacggcgt ttcgcctgcc agtgtcgcgt cgcgcgcaga aacgacgcgg
     7501 tgagcaccag cgccgcttcg aggtcgacgc gcagcacggc agcgagttcg tcggcttcca
     7561 tcgcttcgat cggaccgatg cggccgagca gcccggcgtt gttgatcagc gtcgcggtcg
     7621 cgtgcctgcg gctcgcctgc tggtgcagcc acgcctccag tcgggcggcc acgcccacgt
     7681 cgtgcgccag atcgagcgcc cattgcgtca ggtgcgcgcc ggccgcggtt gcgacttcgt
     7741 cgagcgaccg atccggatgg cgggcgatcg tcagcagcgt gcggtcgcgc tccatcagct
     7801 gctgcgccag cgcgcgcccc aggccgcgcg atgcaccggt gatgatgtag aggtccacgc
     7861 tcacacccct tgcttgaggc ttgcctcgat gaactgatcc agatcgccat cgagcacgga
     7921 ctgcgtgttg ccgacctcga tattggtgcg caggtccttg atgcgtgact ggtcgagcac
     7981 gtagctgcgg atctggtggc cccagccgat gtcggtcttg ccttcctcga gcttggtctg
     8041 ctccaccatg cgcttgcgca gctcctgctc gtacaggcgg gatttgagca tcgccatgca
     8101 ctcggcgcga ttgcggtgct gactgcggtc gttctggctc tgcacgacga tgccgctcgg
     8161 caggtgcgtg atgcgtaccg cggactcggt cttgttcacg tgctggccgc cggcgccgga
     8221 ggcgcgatac acgtcgatgc gcaggtccgc cgggttgatc tcgatctcga tcgtttcgtc
     8281 gacctccggg tacacgaaca cgctggcaaa tgacgtatgg cggcgtgcgt tggagtcaaa
     8341 tggcgacttg cgcaccagcc gatggatgcc ggtctcggtg cgcagcgtgc cgtacgcgta
     8401 ggcgccggaa agcttgatcg tcgcgccctt gatgcccgcg acctcgccgg gcgtctcgtc
     8461 gagtaactcg gccttgaagc ccttgcgctc ggcgtagcgg aggtacatgc gctcgagcat
     8521 gctggcccag tcctgcgcct ccgtgccgcc ggcgccggcc tggatctcga tgaagcagtt
     8581 gttcggatcc agcggattgt tgaacatccg ctgaaactcg agttccgcca cctgcctgcc
     8641 gaggctgccg gcatcgacct cgatcgagat cagcgtcgcg tcgtcgttgt cggcgcgcgc
     8701 catctcgaac agttcctgcg tatcgcgcag accgccggtc agccgctcca tgcggctgac
     8761 cacatcgtcc agcagcttct tttccttgcc gagttcctgc gcctgcttgg gattgtccca
     8821 gacgttcggg ttctcgagtt cgcgcgcaac ctggctcagc ctgtgcgcct tggcatcgac
     8881 gtcaaagata cctccgcagc tcggccgcgc gagcgttgag gtcggtgatg ctggcggaaa
     8941 gctgattcag gcgttcgggt tccatgggca ctctctgaga aaacgcgatt atagaaaccg
     9001 tccccggcgc tgccgcgctg gtctgcgggg agcggggcac ttgcggggct tcttcgccat
     9061 cggcaggggc gccgcgctgg agcattgcag caggtcgccg gcgctcctgc cgcaggggtc
     9121 accccgaaat gggccgtcac gccagtgcgt gctcgatgaa aagcgtgact cgccgctggc
     9181 cctggtagtc gtccgccgcc gggcggtacg ccagtcgggc ccgcgacgga agcgtatcgg
     9241 tatgccggaa ccacatcgca ccgaagcgcc gaccctgcaa ttcgaccgtg agcttcaggt
     9301 gctgctcctt gatcagccgc tgctcgacga tcgcgaactc gttgtcataa agcggtggcg
     9361 gaaagccctg gccccagatc tggcggtcga tcgcctcgac cagcccgagc gagatctcgt
     9421 cggccacgag cgggccgtcg gtgtcgacgg tgcgccggaa cagcgtcgca tcggcggtgg
     9481 cacgcgtggc ttcctcgaac gcggacgcaa agccctcgta gtgctcggcg cgcagcgtca
     9541 ggccggccgc catcgcatgg ccgccgaact tctcgaccag tcccggcgcg agcttggtca
     9601 cgagatcgag cgtgtcgcgc aggtgcacgc cttcgatcga tcgccccgat ccgcgcagcg
     9661 tcgcttcacc aacgcgcgcg cacgcgaccg tggggcgatg gaacctctcc ttcacgcgcg
     9721 aggcgaccag gccgaccacg ccctggtgcc agccgtcgtt gaacagcgtg atcgtgcggc
     9781 gttcggcgaa cgatgcgtcc tgcaagtcgg ccagcgcgtc gaattgcatg tcgacctcga
     9841 tcgcgcggcg ttcgcggttc agctcgtcga gctggcgcgc cagtgccagc gcgcgctcga
     9901 agtcgtcggc gagcaggcac tcgatcccgg tcgacatgtc ggcgagtcgc cccgccgcgt
     9961 tgatgcgcgg cccgagcgca aagccgagat cgctcggccg cgccagttgc gccgcccgac
    10021 cggcgacctg aaacagcgcg gcgatcccgg gctgcatgcg ttccgagcga attcgctcga
    10081 gccctgccgc caccaggatg cgattgttgt ggtcgagccg cacgacgtcg gccaccgttc
    10141 ccagcgcgac gaggtcgagc agcgcgtcga gccgcggttg cgtactttcg gtaaagcggc
    10201 cgcgcgcgcg cagctcggcg cgcagtgcca gcagtacgta gaacatcacg ccgacgccgg
    10261 ccaggtgctt gctcgggaag ccgcagcccg gctggttcgg attgacgatc gcggccgcgg
    10321 ctggcagctg cggtccgggc aggtgatggt cggtgatcag cacgtcgatg ccgagctctc
    10381 gggcgcgcgc cacgccgtcg acgctggcaa tgccgttgtc gaccgtgatc agcaggtccg
    10441 gcctgcgctg cgaagcgagc gtgacgatct cgggcgtcag tccataccca tattcgaaac
    10501 ggttcggcac caggtaatcg acgcgcgccc cgagcgcgcg caggccgcgc acgccgacgg
    10561 cgcacgcggt cgcgccgtcg cagtcgtagt cggcgatgat cgtcatgctg cgctgtgcgt
    10621 cgatcgcgtc ggccagcagg cgcgccgcgt cttcgacgtg cgcgagctgc ggcggcgcga
    10681 tcagatcgcg cgtgaccagc gtcaggtcct gcagcgaccc caccccgcgc gccgcgagcg
    10741 cgcgcgccag cgggcggggc aggccagcgt cggcgagccg ctgcgcggcg gcggcatcga
    10801 acacgcgcgg gacgaaccgt accgcgctca ctgcgccacc ggttcgccga gcagcgcggc
    10861 gagcgcgccg ccggaggggc ggcgccagaa acgcagccga tcgtgtcgcc gcaacgacag
    10921 cacgcgcaag aattgccggc cggtcagaac cagttcgatg ctgttgaaac cgcgccccca
    10981 tgcacggtcg cgcagtcttt ccagccgctg ctcgaatgcg ccgaggcgtt cgatccacac
    11041 gccccacgcg tcgaatgccg ccggctcggc tagatcggtc cacagcgaca ccgcgtctcc
    11101 tggctgactg ggtaggtcat cttcgtcgta cagcgacgcg ggtggaagtc cggccgccag
    11161 cgcccaaccg cgcagcgttg cgtccgcgga cacgatcgat gcgaacggcg cacgcggcag
    11221 cggcgcccac acgcccccgc cgtgcagcca tacgccattg accggctgca gaccgcgctg
    11281 ttcgcgcgcc gtgttgaccg cgtggtgatg ccagcgcacc tggatctcgg tcagcacctt
    11341 gcgccagcgc ccggcgtcgg gtccgaccgg caggcagggg ccaagtggct ggccgcgcgc
    11401 tgacgcgagc ggggcggtat cgagccgcca gggccggtcg gtctgcatga accacgcgtc
    11461 gtgtcggagc cgcttcagca ggccgccgtg ctcgcgcagc accgggtcgg cctcggcggc
    11521 gagcgcatcg gcctcggcat cggtgagttg cgcgtccgca ctgcggccaa tcagcaactg
    11581 gtcgcggccg acgtcgatgt ggatcggctc gcagtgccac cactgcgagc tatcgtcagg
    11641 cagcagcgcg ctggcatggt ttgcggcgcg ccacgcatac ggggcagtca ccggaatcac
    11701 gccgtcgccg cagaacgcgc gccacagcca ggcgaggtgc acggcgccgg ccggcgcgtc
    11761 gcgcaccgat tcgtcaggcg cgcgctgcgc gcgtacgagg caatcggcga tccactctgc
    11821 gtgcgccgcg ccgacgatgt cggctgccag cggcgcggga accaggctgc ccgggacgag
    11881 gacaaaccat ctcatcgcgg cgattctacg gagacatcgc cgtgccgcta gaatcgccgc
    11941 gctcgcgaag ccgccaattg aatcttccgt acgaagtccg catcggcctg cgctacacgc
    12001 gcgcgggacg ccgcgcgcgc cggcgcaacg gcttcatctc gttcatctcg gcaatctcga
    12061 tgggcggcat catgctcggc gtcgccgcgc tgatcgtcgt cctctcggtg atgaacggct
    12121 tcaccaagga ggtgcgcgat cgcatgctgt cggtcgtgtc gcacgtcgaa ctgtcgtcgt
    12181 tcagcggcgc gctcggcgac tggcgccagg tcgcacaacg cgcacggacc aatcccgagg
    12241 tcatcgcggc cgcgccgtac gtgaacgcgc aggcgatgct gacgcgcgac gacgtggtgc
    12301 gcggcgcgct gatccgcggc atcgatccgt cggtggaggg cggcgtgtcc gacatcgtga
    12361 ggaacctgcc gagcggcaag atcatggatc tcgccgaggg cgagttcggc atcgtgccgg
    12421 ggatcgaact ggcgcgcgcg ctcggggtat cggtcggcga caagatcacg ctgctcgcgc
    12481 cgcagggcaa cgtgacgccg gccggcgtgg tgccgcggct caagcagttc cgcgtggtcg
    12541 gtttgttcga atcagggcac tacgagttcg acagcacgct tgcgctggtg cacatcgccg
    12601 acgcggaaaa gatgttccgc gtctcgggtc cgacgggggt gcggctgaag ctgaaggacg
    12661 cgctcgaggc cccgcaggtc gccgaacagc tggcgcacgg gttcgacgcc gacatgcagg
    12721 tgcgcgactg gtcgcgccag aaccgcacct acttcgccgc ggtcaagact gagaagcgga
    12781 tgatgttcat catcctgacg ctgatcgtcg cggtggctgc gttcaacctc gtgtcgacgc
    12841 tggtgatgac ggtgacggaa aagcagtccg acatcgcgat tctgcgcacg ctcggtgcca
    12901 gcccgcgcag cgtgatgtcg gtgttcatcg tgcagggcgc actgatcggc gtgttcggca
    12961 cccttggcgg cgtcggcctc ggcctgctgc tggcgaacaa cctcgacgtg atcgtgcccg
    13021 cgatcgagca cgcgttcggt gtgcagttcc tgccgaagga catctacttc ctgaacagcc
    13081 tgccgtcgga tccgcgcgcg ggcgacatcg tgccgatcgc gatcatctcg ctggtgcttt
    13141 cgctggtggc cacgctgtac ccgagttggc gcgccgcgcg cacgcgaccg gcggaggcgc
    13201 tgcgctatga ctgaagtcat cgccgcgcgc ggcatcttca aggcctaccg cgaggaccgg
    13261 ctcgtggtcg aagtgctgaa gggcgccgat ctcgaggtca ggcgcggcga ggtgatcgcg
    13321 atcgtcggcg cgtcgggttc cggcaagagc acgctgctgc actgcctcgg cgggctcgat
    13381 gcggtcgacc gcggcgcggt gcggatcggc ggccgcgatg tagccgcgct cggggagcgc
    13441 gagcgcggcg acctgcgcaa ccgcgcgctc ggtttcgtct atcagttcca tcacctgctg
    13501 ccggagttca ccgcgctcga caacgtggcg atgccccttt cgatccggcg cctgccgcta
    13561 gaaacggcgc gcgagcaggc ggcggcggcg ctcggtgcgg tcgggctcgg cgagcggctg
    13621 cagcaccatc cggcggagtt gtcgggcggc gagcgccagc gcacggcgat cgcgcgcgcg
    13681 atcgtgacga agcccgcatg cgtgctggcc gacgaaccga cgggcaacct cgatcgcgaa
    13741 accgcgcgcg gcgtgttcga cgtcttcctg gcgctggcgc gctccagcgg taccgcaatc
    13801 gtgatcgtca cgcacgatcc ggaactggca gcgcagtgcg atcgcacgat gcgcctgcgc
    13861 cagggcgtgc tgtcctctgc gtagcgtcag ctgtcgaaga tcgatcccca cagcaggcag
    13921 gcgacaccga gcactgcgag catcacggct gccacgccga ggatcttcgg cagcagcacg
    13981 gcagcttcgc cgcttgccat catttcccgc accggcatgc cgtgcaaggc tgccagcacc
    14041 gcggcgatgc agccgaacga aacggcgccg gcgagggcca gcgcaccgaa cttgaacggc
    14101 atcggccggc gcgccagcaa cagcgcgacg aacgcgccgg caccgagcaa ggcgccaaaa
    14161 ccgatcgctt cgagatcgaa caggtagccg agcagcgccg ccagcagcaa cggccaggcg
    14221 cggtgaagga ttggcatcac acgccgctcc cggcgcgccg tttgcggcgg gcgtgccacg
    14281 cgtggcgcag gtgcaggttc cagatcaccc gcacgatcag gtagccgctc agggagagca
    14341 gcgatgccag caggagcacg ccaagcgcaa ggggggcgcc gagaccgatc atccattggc
    14401 tgaaggcctc ggccgacgcg gcgggctgcg tccacaccca gtccggcggc ggctcgggct
    14461 ccatgccgct cgcggccccg agcgcgaacg agccgatctg gtaggcgatg aagtaaagcg
    14521 gaacgatcgt cagcgggttc gtgtacagcg tggtcagcag cgcgatcggc agattcattc
    14581 gcagcgtcag cgccgcaatg ccggcgccga tcatctggaa tggccccggg atcaggccgc
    14641 agaacagccc gatcgccacg cctcccgccg ccgtgcggcg gttcaggtgc caaagcgccg
    14701 ggtggaagat ccgctcgccg aacagctgga atgccctgac ctctcgtacc ttgtcggcgc
    14761 tcggcatgta gcgcttgaag aacctgcgaa gcatgtacca agtctagtcg gcgatcggca
    14821 atccttcttc ggcagcgcgt ctccgcggct cggatatgct gccatccgac cgttggagac
    14881 ggccgactca tgtggctcga cacgcattgc catctggacg ttcccgaatt cgacgccgat
    14941 cgcgacgcgg tcgtcgcgcg cgcacggcag gcgggcgtgt cgatgatggt gattccggcg
    15001 gtcgaggttg cggcctttgc gcgcgtgaag gaatgcgcgc actgctgcgg ctacgcgtac
    15061 gcgcttggca tccatccgct ctacgtcgaa cgcgccgagg aagacgatat cggacgactc
    15121 cgtgcggcag tggaggaggc gcttgccgat ccgcgcttcg tggcaatcgg tgagatcggg
    15181 ctcgacaact acgttgccac gccgccgcgc gagcgtcagc agtggttcta tgcagaacag
    15241 ttgaagatcg c
//
LOCUS       DBNHBY010000467         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1856542, whole genome shotgun sequence.
ACCESSION   DBNHBY010000467 DBNHBY010000000
VERSION     DBNHBY010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1856542"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC16HB_11580"
     CDS             complement(<1..863)
                     /locus_tag="AC16HB_11580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5607476.1"
                     /translation="MTIELRAADGGTVQVSPEILQTFRAGFRGPVLGPDAPEYEETRK
                     IWNAMIDRRPGLIARCTGTTDVVAAVRFARQHQFLVSVRGGGHNIAGLAVCDGGLMID
                     LSPMKGVWVDPRSRTARAQAGCTLGDLDRETQLHGLAAVLGFVSATGIAGLTVGGGFG
                     YLTRKHGWTCDTLVSMEVVTAEGKVVRAAADENAELFWALRGGSGNFGIVTSFEYRLF
                     PVGPEILGGAIAWRAEDAQEVLSFFREFTAQTPRETTCVAALRIAPPAPWLPKEIHGK
                     PIVAVFVCHSG"
     gene            complement(891..1385)
                     /locus_tag="AC16HB_11585"
     CDS             complement(891..1385)
                     /locus_tag="AC16HB_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5607477.1"
                     /translation="MKRKRLLSFSLGALLAAAGAAMPTLSVAVHPEGFAMSKGADIKW
                     SDVPSLPPGASAAVLEGPMNEASPFTARLRFPANYRIPAHWHPAIERVTVLKGILHVG
                     HGETFDAAKAPPLRPGDFTLIPAKTPHFAYTTNEPVEIQLHGTGPWGITYLNPADDPR
                     ARQQ"
     gene            complement(1552..>3030)
                     /locus_tag="AC16HB_11590"
     CDS             complement(1552..>3030)
                     /locus_tag="AC16HB_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5607478.1"
                     /translation="RAAIGFGDVQWRLGIEGSTAVVLIREALAKSAPEATRERASLLT
                     ALCLALLFSDRQDEAEAAFREAVAMARGCDDPWTLFRALSAILPDRSPERLQLRIAAA
                     REAIALTQRAGHPEWVVPYLTGWHIGDLMESGDTAAATATARVHLTAGTTTREPFLDA
                     VALAALSMIAAHEGRFAEAEQLAIRAVRCGTRFDRANAAGIFSVQMFTIRRQQGRLGE
                     LTPILKQFLDSGSAAATWRPGLAILHCELGARAEARAVFEMLASDDFAAIANDAIRIA
                     SIAYLAETCAWLNDAARASTLYDLLLPYAERNIVFGAHTASFGAAARLLGMLAATLER
                     WDDAERRFEYAIAFDTRTGGRPWLARSRCEFAAMLLKRGRAADLDRATQLLAAALVDA
                     RALGMHGLEQRIVALQAPPSAAGIQDVRSRAKRIAGLSEREVQVLRLIAAGMTNQQIA
                     DHLSRSPSTVAIHVRNILSKTQAANRAEAAAFAVRHGLIARG"
BASE COUNT          498 a         1027 c         1020 g          485 t
ORIGIN      
        1 ctgccgctgt ggcagacgaa gactgcaacg atcggcttgc cgtggatctc cttcggcaac
       61 catggcgcgg gtggcgcaat gcgcagtgcg gccacgcagg tcgtttcgcg cggcgtctgc
      121 gcggtgaact cgcggaagaa cgaaaggact tcctgcgcgt cctcggcgcg ccaggcaatc
      181 gcgccgccga ggatttccgg gccgaccggg aacagacggt attcgaacga agtgacgatg
      241 ccgaagttgc cgctgccgcc gcgcagtgcc cagaagagtt ccgcgttttc atcggcagcg
      301 gcgcggacga ccttgccctc ggccgtcacg acttccatcg acaccagcgt gtcgcaggtc
      361 cagccgtgct tgcgggtcag gtagccgaag ccgccgccca ccgtcagtcc ggcgatcccg
      421 gtggccgaga cgaagccgag caccgcggcg agtccatgca gctgagtctc gcgatcgagg
      481 tcgccgagcg tgcaaccggc ctgcgcgcgc gcggttcggc tccttggatc gacccacacg
      541 cccttcatcg gcgacaggtc gatcatcagc ccgccatcac agaccgcgag cccggcgatg
      601 ttgtggccgc cgccacgaac cgagacgaga aactggtgct ggcgcgcaaa ccgtacggcg
      661 gcaacgacat ccgtcgtgcc cgtgcaacgg gcgatcaggc cggggcggcg gtcgatcatc
      721 gcgttccaga tcttgcgcgt ctcctcgtac tccggtgcgt cggggccgag cacaggaccg
      781 cggaatccgg ccctgaatgt ctgcaggatc tcgggcgaga cctgcacggt gccgccatcg
      841 gcggcgcgca gttcgatggt catgggggct cctgcgattc gttcgcgaga ttactgctgc
      901 ctggcgcgcg gatcgtcggc cggattcaga tacgtgatgc cccacggtcc cgtgccgtga
      961 agctgaatct ccaccggctc gttcgtcgta taggcaaagt gcggcgtctt cgccggaatc
     1021 aacgtgaaat cgcctggacg cagcggcgga gccttggctg catcgaatgt ctcgccatga
     1081 ccgacgtgca agatcccctt cagaacggtc acgcgctcga tcgccggatg ccaatgagcg
     1141 gggatgcggt agttggcagg aaagcgcagg cgcgccgtga acggacttgc ctcgttcatc
     1201 ggtccttcga gcactgcggc gctcgcaccc ggcggcaacg acggcacgtc ggaccacttg
     1261 atgtcggcac ccttggacat cgcaaagccc tccggatgga ccgcgaccga cagtgtcggc
     1321 atcgccgcgc cagccgcggc gagcagggct cccagggaaa atgaaagcag acgcttgcgt
     1381 ttcatgagct gactcctcgg ttgtcacgcc ggccttgcct tccgagtgtg ctgcgcgccg
     1441 gccggcatct gcgggcgcga gcgcattctg ggcaacggcc atccccgtgt aatcgaccag
     1501 actgacgatt cttcggcgcc cctcccatcg gcgcgcgatc gtcagatctg actatcctcg
     1561 ggcgatgagc ccgtggcgga ccgcaaacgc cgcggcctcc gcgcgattcg cggcctgcgt
     1621 cttgctcagg atgttgcgca cgtggatggc cacggtgctc gggctgcgac tgaggtggtc
     1681 cgcaatctgc tgattggtca tgccggccgc aatgaggcgc aacacctgca cctcgcgctc
     1741 ggacaagccg gcaattcgct ttgcgcgcga gcggacatcc tggatgcccg ctgccgatgg
     1801 cggcgcctgc agcgcaacga tgcgctgctc caatccgtgc atgccgagcg ctcgcgcatc
     1861 gacgagcgcc gcggcgagca attgcgttgc gcgatcgagg tccgcggcgc gccccctttt
     1921 cagcagcatc gcggcgaact cgcagcgcga gcgggccagc caggggcgcc cgccggtgcg
     1981 cgtatcgaat gcgatcgcgt actcgaaacg gcgctcggcg tcatcccagc gctcgagcgt
     2041 ggcggccagc atgccgagca ggcgggcggc ggcgccgaac gacgccgtgt gcgcgccgaa
     2101 cacgatgttg cgctccgcgt acggcaagag caggtcgtac agcgtcgacg cacgcgccgc
     2161 atcgttcagc caggcacagg tttcagcgag gtacgcgatg ctcgcaatcc ggatggcgtc
     2221 gttggcgatg gctgcgaagt cgtcggatgc cagcatctcg aagacggcgc gtgcttcggc
     2281 gcgcgcgccc agttcgcagt gcaggatcgc caggcccggc cgccacgttg cggcagccga
     2341 tccgctgtcg aggaactgct tcaggatcgg cgtcaactcg cccagccggc cctgctgacg
     2401 ccggatggtg aacatctgaa cgctgaagat gccggctgca ttggcgcggt cgaaacgcgt
     2461 gccgcatcgg accgcccgaa tggccagttg ctcggcttcg gcaaagcgtc cctcgtgcgc
     2521 ggcgatcatc gatagcgcag caagcgcaac cgcatccaga aagggttcgc gcgtggtggt
     2581 gcccgcagtc aggtgcacac gcgccgttgc ggtcgcggcc gcggtatcgc cggactccat
     2641 caggtcgccg atgtgccagc ccgtgaggta tggcacgacc cactcgggat gtcccgcgcg
     2701 ctgtgtcagc gcgatcgcct cgcgcgccgc agcgatgcgc agttgcagcc gttcgggcga
     2761 acggtcgggc aggatggcag acaaggcgcg gaacagcgtc cacggatcgt cgcatccgcg
     2821 cgccattgcg acggcttcgc gaaatgccgc ctcagcttcg tcttggcggt ccgagaacag
     2881 cagcgccagg cacaatgcag tcagcagcga cgctcgttcg cgcgtcgctt cgggtgccga
     2941 cttcgcgagc gcttcgcgaa tcagcacgac cgcggtcgag ccttcgatac ccagccgcca
     3001 ctgcacgtcg ccgaacccga tcgccgcgcg
//
LOCUS       DBNHBY010000468         5755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1316137, whole genome shotgun sequence.
ACCESSION   DBNHBY010000468 DBNHBY010000000
VERSION     DBNHBY010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1316137"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..749)
                     /gene="gph"
                     /locus_tag="AC16HB_11595"
     CDS             complement(66..749)
                     /gene="gph"
                     /locus_tag="AC16HB_11595"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017962.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR5607479.1"
                     /translation="MIRAVLIDLDGTLMDTAPDLAEAANRMRADFDLPPLPQARVAAF
                     VGKGADVLVHRALTDDLDGQAAADAFARGRAAFYRHYHATNGESAVVFHHVPEALQLM
                     RGRGLKLACVTNKPREFTVPLLDRVQLARWFDTVVAGDDVKEKKPHPALLLAACERLG
                     APPGEALMIGDSINDARAARAAGCSVVLVETGYNEGEHVGTLAGQPGVFAIVPTLLDA
                     AQTVRKLIH"
     gene            complement(746..1121)
                     /locus_tag="AC16HB_11600"
                     /pseudo
     CDS             complement(746..1121)
                     /locus_tag="AC16HB_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            complement(1201..1929)
                     /gene="rpe"
                     /locus_tag="AC16HB_11605"
     CDS             complement(1201..1929)
                     /gene="rpe"
                     /locus_tag="AC16HB_11605"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5607480.1"
                     /translation="MDSRIAPSILSADFARLGEEVRNVVAAGADWIHFDVMDNHYVPN
                     LTIGPLVCEAIRPHVSVPIDVHLMVEPVDALVPMFAKAGANVITFHPEASRHIDRTLA
                     LIRDQGCKAGLVFNPATPLGWMDHVMDKLDIVLLMSVNPGFGGQAFIPEALAKLGQTR
                     ARLEAYRRATGKAILLEIDGGVKVDNIRSIAAAGADTFVAGSAIFGAKDYKATIDAMK
                     REIAAAQRVTTQRAAQEDSVYRGA"
     gene            2022..2396
                     /gene="apaG"
                     /locus_tag="AC16HB_11610"
     CDS             2022..2396
                     /gene="apaG"
                     /locus_tag="AC16HB_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR5607481.1"
                     /translation="MAKYEFHVSVQPQYLPEQSDPADDRYVFAYTVTVRNTGEVTAQL
                     LARHWIITDADGKVQEVQGEGVVGEQPILRPGEAFQYTSGCPLETPVGSMRGKYFCVA
                     EDGTRFDADIPEFVLSMPRTLH"
     gene            2497..3750
                     /gene="mltA"
                     /locus_tag="AC16HB_11615"
     CDS             2497..3750
                     /gene="mltA"
                     /locus_tag="AC16HB_11615"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR5607482.1"
                     /translation="MEVGRASVAWNCRWAWSALGALLLAACVTPPQQPATPPAVKTVT
                     LARYEAIAFETLPALADADLAGAWPALLASCRALERNARRADWLPFCSAARQADAGNP
                     RALRALLAAQLQAWRIVAVTREDLGTDAKAEPRELVVTDRGRLTGYYEPQLAGSRARS
                     VPFVHPLYRVPDDLLTIELGALYPELAGRRVRGRLDGRRVLPYWSREQIESGARLAGS
                     ELLWTDDALEAFFLQVQGSGRVRLNDGSVVRVGYADANGHPYHSIGRILVERGELKVE
                     EASLQGIRAWARAHPDRLSELMNQNPSYVFFRELPLGDPNAGPIGAMNLALTPGYSLA
                     ADPQFIPLGSPVVIHTVHPASGAPLARMMLAQDVGGAIRGPLRFDFFWGFGAEAGELA
                     GRQRHDVRAWLLLPRGVTPEQMLAR"
     gene            complement(3754..4515)
                     /locus_tag="AC16HB_11620"
     CDS             complement(3754..4515)
                     /locus_tag="AC16HB_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR5607483.1"
                     /translation="MHRILLALVASLVATFAAAQPAQPARAAKPTSVEETIRARVEER
                     FGVKPTDVKLMPFGLYEVTIGTDVAYVDKEVAYLFLGRVVDAKSKEDLTQGRRDELMK
                     VDYKSLPFDQAFKIVRGDGSRQFAVFADPNCPYCKKFEKDLADMKNVTIYTFLYPILS
                     RDPSSPTDSFERARNVWCAKDRKTVWEGLMLDGKAPPVASADCKHPLQQNLALGQKLE
                     VSGTPTIFFVDGRRLPGAVPVERIEAGLARAVGKK"
     gene            complement(4579..>5755)
                     /locus_tag="AC16HB_11625"
     CDS             complement(4579..>5755)
                     /locus_tag="AC16HB_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5607484.1"
                     /translation="DRQPAYDLAVVGAGVAGLAAALGAAQLGLKVALIGPDARAFAPT
                     EAVPFDARIYALAPSAIDLLERLKAWPQMDTARVQSVARMRVFGDAGSELTFDAYQAA
                     LERLAAIVEEGELARVLAAACSYAPGITRLRASFTRLQREAGAARVELDDGAALDARL
                     VVGADGAQSAVRAAAGINADETSYRQTALVANFDCTRPHQGVAFQWFCDEGVVALLPL
                     PGDFVSLVWSAPEQLAAELQALEAAGFAQRVTARTQSIIGELALRGGVHAFPLRVIRV
                     DRLVEERMALIGDAAHVVHPLAGQGLNLGLQDVSELLRVLAAREEFRDPGDRVLLRRY
                     ARARAESIDLMRFTTDALARLFGADNALARELRNAGLSLVDRITPLKNALIRHAATR"
BASE COUNT          876 a         2034 c         1955 g          890 t
ORIGIN      
        1 ctgggcatgc caaccgcggc accaggccca gagatccaga tcaacccgtt tgtcgaactg
       61 acgcatcagt ggatcagctt ccgtactgtc tgcgctgcgt cgagcagcgt gggcactata
      121 gcaaagaccc ccggttggcc cgcaagcgtg ccaacgtgct cgccctcgtt gtagccggtc
      181 tcgaccagca ccacgctgca gccggccgcg cgcgcggcgc gcgcatcgtt gatcgagtcg
      241 ccgatcatca gcgcttcgcc gggcggcgcg ccgagccgct cgcaggcggc gagcagcagc
      301 gccgggtgcg gcttcttttc cttcacgtcg tcgccggcga cgaccgtgtc gaaccagcgc
      361 gccagttgca cgcggtcgag cagcggcacc gtgaactcgc gcggcttgtt cgtcacgcag
      421 gcaagtttca ggccgcgtcc gcgcatcagc tgcagcgcct ccggcacgtg atggaaaacg
      481 accgcgcttt cgccgttggt cgcgtgatag tggcgataga acgccgcgcg tccgcgcgcg
      541 aacgcgtccg cggcggcctg cccgtccagg tcatccgtca gcgcgcgatg caccagcacg
      601 tcggcgccct tgccgacgaa cgccgcgacg cgcgcctgcg gcagcggcgg cagatcgaag
      661 tccgcgcgca tccggttggc ggcttcggcc agatccggcg cggtatccat cagcgtgccg
      721 tccagatcga tcagcaccgc gcggatcatg tcggccggtg cgcgagcaag tcgacctcga
      781 cgcgcgcgcc tttcgcgagc gcggtcacgc cgatgcaggt gcgcgccggc agccttcccg
      841 gcgaagtagc tcgcgtagac cgcgttgaag cgcgcgtagt cttcttcgaa gtgcgtcagg
      901 tatgcgcgcg ccgacaccac gtgctcgagc cccaggccga cgccggcaag cacgagcacg
      961 aggttgtcca tcacgcgccg tgtctgctcg tcgatccctt gcggcagtgg cgcgtcgggc
     1021 gcgccgggca gcgggggcat ctgcccggtg acgaacaccc agccgtcgac cttggtggcg
     1081 tgcgagaacg gcgccaccgg cgccggcgcc gcgtcgacca tgtgaaaccg gatcgggctc
     1141 agcggcctgc cctccgcgct gcgcgccgcg ggatatgcgc ttccgggcgg ccgcacgcgc
     1201 tcatgcgccg cggtagacgc tgtcttcctg cgcggcgcgc tgcgttgtca cgcgctgtgc
     1261 ggcggcgatc tcgcgcttca tcgcgtcgat cgttgccttg tagtccttgg cgccgaagat
     1321 cgcgctgccg gcgacgaagg tgtcggcacc cgcggcggcg atcgaccgga tgttgtcgac
     1381 cttcaccccg ccatcgatct cgagcaggat cgccttgccg gtcgcacgcc ggtacgcctc
     1441 gagccgcgcg cgcgtctggc ccagcttggc gagcgcttcg ggaatgaacg cctgcccgcc
     1501 gaaacccggg ttcaccgaca tcagcagcac gatgtcgagc ttgtccatca cgtggtccat
     1561 ccagccgagc ggcgtggccg ggttgaacac cagcccggcc ttgcagccct ggtcgcggat
     1621 cagcgccagc gtgcggtcga tatggcgcga cgcctccgga tggaaggtga tcacgttggc
     1681 gccagccttg gcgaacatcg gcaccagcgc atcgaccggt tcgaccatca ggtgcacgtc
     1741 gatcggcacc gagacatgcg gtcggatcgc ctcgcacacc agcgggccga tcgtcaggtt
     1801 cggcacgtag tggttgtcca tcacgtcgaa gtggatccag tcggcgcctg cggcgacgac
     1861 gttgcgcact tcctcgccca gacgcgcgaa gtcggcggac aagatgctgg gcgcgatgcg
     1921 agaatccata gcggtcgtcc ggttgcgggt tcgtcccgat tctacggaac gaccaaggga
     1981 atcaaaggtc acggatcaca gatcacggat cacgaacccg gatggccaag tacgaattcc
     2041 acgtcagcgt gcagccgcaa tacttgcccg agcaatcgga cccggccgac gaccggtacg
     2101 tgttcgccta tacggtcacg gtacgcaaca cgggcgaggt gaccgcgcaa ctgctggcgc
     2161 gccactggat catcaccgac gccgacggca aggtgcagga ggttcagggc gaaggggtgg
     2221 tcggcgaaca gccgatcctg cggcccggcg aggcgttcca gtacaccagc ggctgcccgc
     2281 tcgagacccc ggtcggctcg atgcgcggca agtatttctg tgtcgccgaa gacggcacgc
     2341 gcttcgacgc ggacattccc gagttcgtac tgtcgatgcc gcgcacgctg cactgatcat
     2401 ttgtcaccgg gcggcttgcg gccatggaac atcgtccacc agacgatgaa gaccagcagt
     2461 gccagcgcga gcagcgcttc gaggatgatg agccacatgg aagtcggtcg ggcgtcggtc
     2521 gcgtggaatt gtaggtgggc gtggtctgcg ctcggcgcgc ttctgttggc cgcgtgcgtg
     2581 acgccgccgc agcagccggc cacgccgccc gcagtcaaga cggtcaccct ggcgcgctac
     2641 gaggccatcg cgttcgagac gctgcctgcg ctcgccgatg ccgatctcgc cggtgcgtgg
     2701 ccggcgctgc tcgcgtcgtg ccgcgccctc gagcgcaatg cgcgccgcgc cgactggctg
     2761 ccgttctgca gcgcggcccg gcaggccgat gcgggcaacc cgcgcgcatt gcgcgcgctg
     2821 ctcgccgcgc aactgcaggc gtggcgcatc gtcgcagtca cgcgcgaaga cctcggcacc
     2881 gacgccaaag cggagccgcg cgagcttgtc gtcaccgatc gcggccgcct caccggctac
     2941 tacgagccgc agctcgccgg cagccgcgcg cgcagcgtcc cgttcgtgca tccgttgtac
     3001 cgcgtgccgg acgatctgct gacgatcgaa ctcggcgcgc tgtatccgga gctggcgggc
     3061 cggcgcgtgc gcggccgcct cgacggacgg cgcgtgctgc cgtactggtc gcgcgagcag
     3121 atcgagagcg gcgccaggct cgccgggtcg gaactgctgt ggacggacga tgcactcgaa
     3181 gccttcttcc tgcaggtgca gggatcgggc cgcgtgcgcc tgaacgacgg cagcgtcgtg
     3241 cgcgtcggct acgcggacgc caacggccat ccataccact cgatcggccg catcctggtc
     3301 gagcgcggcg agctgaaggt cgaggaagca tcgctgcagg gcatccgcgc atgggcgcgc
     3361 gcgcatccgg accgcttgtc cgagctgatg aatcagaatc cgagctacgt cttcttccgc
     3421 gaactgccgc tcggcgatcc gaacgcaggg ccgatcggcg cgatgaacct cgcgctgacg
     3481 cccggctact cgctggcggc ggatccgcag ttcatcccgc tcggcagtcc cgtggtgatc
     3541 cacaccgtgc atcccgcgag cggcgcgccg ctggcgcgca tgatgctggc gcaggacgtc
     3601 ggcggtgcca tccgcggtcc gctgcggttc gatttcttct ggggcttcgg tgccgaggcc
     3661 ggcgaactcg cgggccgtca gcgtcacgac gtgcgggcgt ggctgctgct gccgcgagga
     3721 gtcacgcccg agcagatgct cgcgcgctga gcgctacttc ttgcccaccg cacgggccaa
     3781 cccggcctcg atgcgctcga cgggaaccgc ccccggcagg cggcggccgt cgacgaagaa
     3841 gatggtcggc gtgccggaca cctcgagctt ctgcccgagc gccaggttct gctgcagcgg
     3901 atgcttgcag tccgccgacg cgaccggcgg ggccttgccg tccagcatca gtccttccca
     3961 caccgtcttg cggtccttcg cgcaccagac gttgcgcgcg cgttcgaacg agtcggttgg
     4021 cgatgacggg tcgcgcgaca ggatcgggta caggaacgtg tagatcgtca cgttcttcat
     4081 gtcggccaga tccttctcga acttcttgca gtacgggcag ttcgggtcgg cgaacaccgc
     4141 gaactgccgc gagccgtcgc cgcgcacgat cttgaacgcc tggtcgaacg gcagcgactt
     4201 gtagtcgacc ttcatcaact cgtcgcgtcg cccctgcgtc agatcttcct tggacttggc
     4261 gtcgaccacg cggccgagaa acaggtaggc gacctccttg tcgacgtagg cgacatcggt
     4321 gccgatcgtc acttcgtaca ggccgaacgg catcagtttc acgtccgtcg gcttgacgcc
     4381 gaagcgttcc tcgacacgtg cgcggatggt ctcttcgacc gacgtcggtt tcgcggctct
     4441 ggctggctgc gccggctggg ccgccgcgaa cgtcgccacg aggctggcga ccaatgccag
     4501 gagaattcga tgcatgggag tccctttcag cgaccgcgat tttacccgcc cacgcccgcc
     4561 gccattgcgg caaacggctt agcgcgtcgc agcgtggcgg atcagcgcgt tcttcagcgg
     4621 cgtgatccgg tcgaccagcg acagcccggc gttgcgcaat tcgcgcgcca gcgcgttgtc
     4681 ggcgccgaac aggcgcgcca gcgcgtcggt ggtgaaccgc atcaggtcga tcgactcagc
     4741 ccgcgcacgc gcatagcggc gcagcagcac gcggtcgccc gggtcgcgga attcctcccg
     4801 tgcggccagc acgcgcagca actccgacac atcctgcaac ccaaggttca atccctggcc
     4861 ggcgagcgga tgcacgacgt gcgcggcgtc gccgatcagc gccatgcgtt cctcgaccag
     4921 ccggtcgacg cggatcacgc gcagcggaaa cgcatgcacg ccgccgcgca gcgcgagctc
     4981 cccgatgatc gactgcgtgc gcgcggtcac gcgctgcgcg aacccggccg cttcgagtgc
     5041 ctgcagttcg gccgcgagtt gctcgggcgc ggaccacacc agcgacacga agtcgcccgg
     5101 cagcggcagc agcgcgacca cgccctcgtc gcagaaccac tggaacgcca cgccctggtg
     5161 cggccgcgtg cagtcaaagt tggctaccag agcggtctgc cggtacgagg tctcgtcggc
     5221 attgatgccc gccgcggcac gtacggcgga ctgcgcgccg tcggcgccga ccacgagccg
     5281 cgcgtcgagc gccgcgccgt cgtcgagttc gacgcgcgcg gccccggctt cgcgctggag
     5341 ccgcgtgaac gaagcgcgca ggcgcgtgat cccgggggca taactgcacg ccgcggccag
     5401 cacgcgcgcc agttcgcctt cctccacgat cgcggccagc cgctcgagcg cggcctggta
     5461 cgcatcgaac gtcaattcgc tgccggcgtc gccgaagacg cgcatgcgcg ccaccgattg
     5521 cacgcgcgcc gtgtccatct gcggccacgc cttcagccgc tccagcaggt cgatcgccga
     5581 gggcgccagc gcgtagatgc gcgcatcgaa cggcaccgcc tcggtcggcg cgaaggcgcg
     5641 cgcgtcgggt ccgatcagcg cgaccttcag cccgagttgc gcggcgccga gcgcggcggc
     5701 cagtccggcc acgccggcac cgacgaccgc gaggtcgtat gcaggctgtc ggtcg
//
LOCUS       DBNHBY010000469         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1585740, whole genome shotgun sequence.
ACCESSION   DBNHBY010000469 DBNHBY010000000
VERSION     DBNHBY010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1585740"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /gene="egtD"
                     /locus_tag="AC16HB_11630"
     CDS             <1..776
                     /gene="egtD"
                     /locus_tag="AC16HB_11630"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR5607485.1"
                     /translation="SIFDAHLPAIAKAYGAPGCMLIDLGAGNCEKAARLFHALQPAAY
                     VALDIAVEYLATSLERLQREYPALQMLGVGTDFTRSLALPEGVSGDRRLFFYPGSSIG
                     NFAPDEAGAFLAQVRGAMADDGALLIGVDLVKDARVLGAAYDDALGVTAAFNLNVLNH
                     VNRLIGADFDIAGWRHVAFYDSKEQRIEMHLEARQANTVRWDGGERRFAAGERIHTEN
                     SYKYSAAQFAALLREAGFRRQQHWADDRGWFALYLAHAE"
     gene            773..1984
                     /gene="senA"
                     /locus_tag="AC16HB_11635"
     CDS             773..1984
                     /gene="senA"
                     /locus_tag="AC16HB_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HIR5607486.1"
                     /translation="MNTDVSTLHSVGRRARHYFRDELRLAMQGAHKRLHALIDDLTDA
                     QWQVPQQAGLNPVAWEAAHVAWFWEFWVQRGPHRRGNDGLIYAARPPIHAGPDALLDS
                     ARLAHAARWTTPLPSRREVEAMLDATLDAALASLALADEDDASLYFYRLALLHESMHN
                     EALTWMRAALGYPAPRGTALPVLTPREAVQIPAETVHVGWPADAGGFSFDNEQPEHEV
                     ALTSYAIDGTPLSAGAFREFVEAGGYARDEYWQGEAAQRWHANCRRAHPERWRRRGGD
                     WEVRWFDQWLPLDPEQPVIHVNAFEAEAYCRWAGRRLPRAAEFEHAALEGRIEWGGTV
                     WEWTADPFEPYPGFVAGPYADYSAPWFHTHRELRGGSFATHARLHHARYRNFALPERS
                     DLFAGFRTATD"
     gene            2040..3005
                     /locus_tag="AC16HB_11640"
     CDS             2040..3005
                     /locus_tag="AC16HB_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607487.1"
                     /translation="MQRRTLLAGLVCAALPVSLRAQAAYPTRPVRIIAPVQPGGGVDL
                     VGRTVAERLTRALGQSFFVENQSGGGGAIAGQTVARAAPDGYTLMVGYVGTHGTNPAV
                     RKLPYDAVNDFTAIAMVGGTPNVLVVHPSVPVTTLQEFVAYARRNPGRLNYGSAGPGT
                     LTHLAMEQFKLAAGFFAVHIPYRGIGPAFTDLLGGQTQAMLPGLAAAIPHIRAQKVRP
                     LAVTGLRRHPLLPDVRTFDELGYKGFDGVQWYGIVGPAKLPDAITQRLNEEINKALAS
                     PELRERLSSEALEPMPMTPAQFAHYIRDDIARWRRLARERHIEIE"
     gene            3008..>3283
                     /locus_tag="AC16HB_11645"
     CDS             3008..>3283
                     /locus_tag="AC16HB_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5607488.1"
                     /translation="MARNTQVAAELDAPPVTVTLARFVATHPSRGWIDAVEHEAQRTF
                     LNWVGCAVGAARHPSALAALAALRELQPAPQATVLGTRERIDMAGAAL"
BASE COUNT          505 a         1140 c         1133 g          505 t
ORIGIN      
        1 catcgatctt cgatgcgcac ctgcccgcga tcgccaaggc ctacggcgca cctggctgca
       61 tgctgatcga cctcggcgcg ggcaactgcg aaaaagcggc gcggctgttc cacgcgctgc
      121 agcccgccgc gtatgtggcg ctcgacatcg cggtcgaata tctcgcgacc tcgctcgagc
      181 gccttcagcg cgagtacccg gcgctgcaga tgcttggcgt cggcaccgac ttcacgcgct
      241 cgctcgcact gccggaagga gtttccggcg accggcggct gttcttctat cccggctcgt
      301 cgatcgggaa cttcgcgccg gatgaagcgg gtgcgttcct ggcgcaggtt cgcggggcga
      361 tggcagacga cggcgcgctg ctgatcggcg tggatctcgt caaggacgcg cgcgtgctgg
      421 gcgccgctta tgacgacgcg ctcggggtca ccgcggcgtt caacctgaac gtgctgaacc
      481 acgttaatcg actgatcggc gccgacttcg acatcgcggg ttggcgccat gtcgcctttt
      541 acgattcgaa ggagcagcgc atcgaaatgc acctggaagc gcgtcaggcc aatactgtcc
      601 gctgggacgg cggagaacgc cgttttgccg ccggcgaacg catccacacg gagaattcgt
      661 acaaatacag cgcggcgcag ttcgccgcgc tgctgcggga agcgggattc cggcggcagc
      721 agcactgggc cgacgaccgc ggctggtttg cgctgtacct cgcccacgca gaatgaacac
      781 ggacgtatcg acactgcact cggtcggacg ccgcgcgcgt cactatttcc gggacgagct
      841 tcggttggcg atgcagggcg cacacaagcg tctgcacgcg ctgatcgatg atctgacgga
      901 cgcgcaatgg caggtgccgc agcaggccgg cttgaacccg gttgcttggg aggcggcaca
      961 cgtcgcctgg ttctgggagt tctgggttca acgcggcccg catcgccgcg ggaatgacgg
     1021 tctgatctat gcggcgcgtc cgccgatcca cgccggcccg gacgcgctgc tcgactcagc
     1081 gcggcttgcg cacgccgcgc gctggacgac gccgctgccg tcgcgccgcg aggtcgaggc
     1141 gatgctcgat gccacgctcg acgcggcgct ggcgtcgctc gcgctggccg acgaagacga
     1201 tgcgtcgctg tatttctacc ggctcgcgct gctgcacgag agcatgcaca acgaggcgtt
     1261 gacgtggatg cgcgccgcgc tcggctaccc cgcgccccgc ggcaccgcgc tgccggtgct
     1321 gacgccgcgc gaggcggtgc agatccccgc cgaaaccgtt cacgtcggct ggcccgccga
     1381 cgcgggcggt ttctccttcg acaacgagca gcccgagcac gaagtcgcgc tgacatcgta
     1441 tgcgatcgac ggcacgccgc tcagcgccgg cgcattccgc gagttcgtcg aagccggcgg
     1501 ctacgcgcgc gacgaatact ggcagggcga ggctgcgcag cgctggcacg ccaactgccg
     1561 tcgcgcgcat cccgagcgct ggcggcgccg gggcggcgac tgggaggtac gctggttcga
     1621 ccagtggctg ccgctcgatc ccgagcaacc ggtcatccac gtcaatgcgt tcgaggccga
     1681 ggcgtattgc cgctgggccg ggcggcgcct gccgcgcgcg gcggagttcg agcatgcggc
     1741 actcgaaggg cgcatcgaat ggggcggcac cgtgtgggag tggaccgccg atccgttcga
     1801 gccgtatccg ggcttcgtcg ccggcccgta cgccgactac tcggcaccct ggttccacac
     1861 ccaccgcgaa ctgcggggcg gcagctttgc cacccatgcg cgactgcatc acgcgcgcta
     1921 tcgcaacttt gcgctgccgg agcgcagcga cttatttgcc gggttccgta ccgcgacgga
     1981 ctgacttggc ggcggcgcat cggtagcatc gcgtccgttc gccatcgccg atccgaccaa
     2041 tgcaacgtcg cacactgctc gccggcctcg tctgcgctgc gctgcccgtc agcctccgcg
     2101 cgcaagccgc ctatccgacg aggccggtgc gcatcatcgc gccggtgcag ccgggcggcg
     2161 gcgtcgacct cgtcggccgc accgtcgccg aacggctgac gcgcgcattg ggccagtcgt
     2221 tcttcgtcga gaaccagagc ggcggcggcg gtgcgatcgc agggcagacg gtggcgcgcg
     2281 cggcacccga cggctacacg ctgatggtcg gctacgtcgg cacacacggc accaatccgg
     2341 ccgtgcgcaa gctgccgtac gacgcggtca acgatttcac cgcgatcgcg atggtcggcg
     2401 gcacgccgaa cgtcctggtc gtgcatccga gcgtgccggt gaccacgttg caggaattcg
     2461 tcgcctacgc acgcaggaat ccgggcaggc tcaactatgg ttcggcgggg cccggcacgc
     2521 tgacgcacct ggcgatggag cagttcaagc tcgcggccgg attcttcgca gtgcacattc
     2581 cctatcgcgg catcgggcct gcattcacgg acctgctcgg cggccagacg caggcgatgc
     2641 tcccggggct ggcggcggcg attccgcaca tccgcgcgca gaaggtgcgg ccgctggcag
     2701 tgacgggact gcgacgccat ccgctgctcc ccgacgtgcg cacgttcgac gaactcggct
     2761 acaagggctt cgacggcgtg cagtggtacg gcatcgtcgg cccggcgaag ctgcccgacg
     2821 cgatcacgca gcggctgaac gaggagatca acaaggcgct ggcaagtccc gagctgcgcg
     2881 agcgcctttc gagcgaggcg ctggagccaa tgccgatgac gccggcgcag ttcgcccact
     2941 acatccgcga cgacatcgca cgctggcggc ggcttgcgcg cgagcgccac atcgagatcg
     3001 agtagacatg gcacgcaaca cgcaggtcgc ggccgaactc gacgcgccgc cagtcaccgt
     3061 tacgctcgct cgctttgtcg ccacgcatcc gtcgcgcggc tggatcgatg cggtcgagca
     3121 cgaggcgcag cgcacattcc tcaactgggt cggctgcgcg gtcggcgccg cgcgccatcc
     3181 gagcgcgctg gcggcgctgg ccgcgctgcg cgaactgcag ccggcgccgc aggcaaccgt
     3241 tctcggcacg cgcgaacgga tcgacatggc cggcgcggcg ctg
//
LOCUS       DBNHBY010000470         4726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_29843, whole genome shotgun sequence.
ACCESSION   DBNHBY010000470 DBNHBY010000000
VERSION     DBNHBY010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_29843"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="AC16HB_11650"
     CDS             complement(<1..582)
                     /locus_tag="AC16HB_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5607489.1"
                     /translation="MSIAATVARRARLQNNRGWRSLSVPPKPQSPSSIVENPSSTSTA
                     LLQPRHPPRTVPQLLAESIGRHAERIAIMAPDRRALTYAELGAQVDAVARALAEAGFG
                     RGQRIAVALPHGPEFPAALLSVCCAATAAPLNDQLTEAALAQSLAAMRIDALIVAEGA
                     ETNATRAARRRGIPQLEVHVPPDAPAGAFELLAR"
     gene            679..1305
                     /locus_tag="AC16HB_11655"
     CDS             679..1305
                     /locus_tag="AC16HB_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR5607490.1"
                     /translation="MAEFALARFRQGTAYEVVGFTVDRVVLREPVLCGLPVVPFEEVE
                     QRFPPATARMFIAVGPVQMNRIRADRYSQARRRGYGFVSYISPHAIIEQDTQIGENVS
                     IGDHVIIAPYTRIGNDVRIGTGAVIGHHCVLEDHCFVGINCSILGSVRIGARALVGAG
                     AVIRDQTSIGEASIVGIGATIVRDTAPGSVHAAPEAIQLSISSERIKI"
     gene            complement(1313..2266)
                     /locus_tag="AC16HB_11660"
     CDS             complement(1313..2266)
                     /locus_tag="AC16HB_11660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607491.1"
                     /translation="MMRRRELLQSLASGLLLHATRQAAAQALPNPIRLIVAYSPGGQS
                     DLVARLLAQKLGSLLGVTMVVENRPGAGGAIGVEYVARAPADGSTLLLGSGSNLSIAP
                     VQDSGLRYDPMRDFAPVGRVARVPLVLAVRSSLPITNVAQLLDYARKHPGQLTYASGA
                     PLVQFAIESLKASAHVDILQVPYKGTAPGMLDAIAGRVDLVLADVAAAVPHVDAGTLR
                     VLANAGPTRPRVLTGVPTMSEQGFDLSFESWQGLLAPSGTPGELIERLRGPLRQALAS
                     KDFRDGLEHLGFEPIDEPPEAFAPFLRNELERYRRLAARAS"
     gene            complement(2263..4155)
                     /locus_tag="AC16HB_11665"
     CDS             complement(2263..4155)
                     /locus_tag="AC16HB_11665"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR5607492.1"
                     /translation="MSALDDDQPPWARPRHPPGAFAQLLATSVRDHAAAVAILAPGKP
                     VLSYVRLGRRLRAIASGLADAGFGRTARVGVALPDGPEFAVALLGVCSTATCAPLNEA
                     LDESALVHLVVAMRLDALIVSADTNAAVLRAAQRAGVPLLRLRSATGQDGDSTLEPIH
                     SPLRRRVESDPPQLDDIALLMHTSGTTGTPKIVPWLQWRVAEAARNRVELARIDESDR
                     CLILLPLHSSAGIRRLLAGVLTGGSNIVPARPTADEIIDLLQTLAPTQLFAPPAAHIE
                     LLEAFERRVPRPKHFLRAIWSGTTELPEAARARLARAFAVPVIVGYGMTESGAIAQTP
                     FPPEQAPPGSAGRAVNIEIAVADESGRFLGPDESGEIVVRGPELFDGYENDAEANRAA
                     FRDGWFRTGDAGHIDREGFIYLAGRLKEVINRGGAKVAPAEVEAALMQHPRVIEAAAF
                     AIAHPTLGQDVAAAVVLRGSLGEGEMRRFLRARLAPFKIPTRIFEVANLPRGSTGKFD
                     RAALAGLAERSSGASFEPPSDHEESALARIFSDVLEAPSVGRKDSFFHLGGDSLRGMR
                     VLATVEAEFGIALTLDVLFDHPTVAELAAAIRKRVHAEPEHATTPTDPPRSAAASAHF
                     QFDGTR"
     gene            complement(4307..>4726)
                     /locus_tag="AC16HB_11670"
     CDS             complement(4307..>4726)
                     /locus_tag="AC16HB_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011507064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactarate dehydratase"
                     /protein_id="HIR5607493.1"
                     /translation="LGNIQKIGKKCTVDGVLDKAEMPTHPGLWFMDSSSAAAEMVTLC
                     AASGFAVHFFPTGQCNVIGNPILPVIKICANPRTVRLMSEHIDVDTSGLLQREITLDQ
                     AGDKLLECMLRTANGRLTAAEALGHREFVLTRLYESA"
BASE COUNT          777 a         1563 c         1612 g          774 t
ORIGIN      
        1 gcgcgcgagc agttcgaacg cgcccgcagg tgcatccgga ggtacgtgca cctcgagctg
       61 cggaatcccg cgccggcgcg cagcgcgggt cgcattcgtc tcggctcctt cggccacgat
      121 cagcgcatcg atgcgcatcg cggccaggga ttgggcgagc gctgcttccg tcagctggtc
      181 gttcagcggc gcagcggtgg ccgcgcaaca cacgctcagc agtgcagccg ggaactccgg
      241 gccgtgcggc agcgccacgg caattcgttg tccgcgtccg aatcccgcct cggccagcgc
      301 acgggcgacc gcatcgacct gtgcaccaag ctccgcatac gtgagcgcgc gccgatcggg
      361 ggccatgatc gcgatgcgct cggcatgccg tccgatcgat tcggccagca gttgcggaac
      421 cgtgcgcggt gggtgcctcg gctggagcaa ggcggtcgac gttgaagaag ggttttccac
      481 gatggatgag ggcgattgcg gtttaggcgg caccgagagg cttcgccagc cgcgattgtt
      541 ctggagtcgg gcgcgtcgag caactgtcgc cgcaatcgac accagagtct gttcccgtat
      601 tcttccggtc cgtcaccgca cttacgatga tctcgcatcg acatggccga accgctcgtc
      661 atctacggaa atggtcagat ggccgaattc gcgctcgcgc ggttccgcca aggtacggcc
      721 tacgaagtcg tcggattcac ggtcgaccgg gtcgttctgc gcgaacccgt tctgtgcgga
      781 ttgcccgtcg tgccgttcga ggaggtcgag cagagattcc cgccggcgac ggcgcgcatg
      841 ttcattgccg tgggtccggt ccagatgaat cgcattcgcg ccgatcgcta cagtcaggcg
      901 cgacggcgcg gctacggttt cgtcagctac atcagtccgc acgcgatcat cgagcaggac
      961 acccagatcg gcgagaacgt ctcgatcggg gaccacgtga tcatcgcgcc ctatacgcgc
     1021 atcggcaatg acgtgcgcat cggcaccgga gccgtgatcg gtcatcactg cgtgctcgag
     1081 gaccattgct tcgtggggat caattgcagc attctggggt ccgtgcgcat tggcgcgcgc
     1141 gcgctggtgg gcgctggcgc ggtgattcgc gatcagacca gcatcggcga ggcgagcatc
     1201 gtcggtatcg gcgcgaccat cgtccgcgac accgcaccgg gcagcgttca tgcggccccg
     1261 gaggcgatcc agctttcgat cagcagcgag cgtatcaaga tctgatgccg catcacgacg
     1321 cgcgcgccgc cagccgccga tatcgttcca gttcgttgcg caggaaaggc gcgaacgcct
     1381 ccggcggctc gtcgatcggt tcgaagccga gatgctcgag gccgtcgcga aaatccttgg
     1441 acgccagtgc ctgccgcagc gggccgcgca gacgttcgat caattcgccg ggcgttccgc
     1501 tcggcgcaag caacccctgc cacgattcga acgaaaggtc gaatccctgt tcgctcatcg
     1561 tcggcacgcc agtcagtaca cgcggacgtg tcgggcccgc gttggcgagc acgcgcaatg
     1621 tgcctgcatc cacgtgcggg acggccgcgg ccacatcggc cagcaccagg tcgacccggc
     1681 cggcgatagc gtccagcatt ccaggcgcag tccccttgta gggcacctga aggatgtcca
     1741 cgtgcgcgga tgccttcagc gactcgatgg caaactggac cagcggcgcg ccggacgcat
     1801 aggtcagctg gccgggatgc ttgcgcgcgt agtcgagcag ttgcgccacg ttcgtgatcg
     1861 ggagactgga acgcacggcg agcaccagag gcacgcgggc aacccttcca acgggtgcga
     1921 agtcacgcat cgggtcgtac cgcaagccgc tgtcctgcac gggtgcgatg gacaggttgc
     1981 tgccgctgcc gagcaacagc gtggagccgt ccgccggagc acgcgcgacg tactcgacgc
     2041 cgatcgcgcc gccggcgccg ggtcggttct ccacgaccat cgtgacgccg agcaggctgc
     2101 cgagcttttg cgcgagcagg cgcgccacca gatcgctttg gccgccgggc gaataggcga
     2161 cgatcagtcg gatcggattc ggcagcgcct gcgccgccgc ctggcgagtc gcgtgcagca
     2221 gcagtccgga tgcgagcgac tgcagcagtt cacgccgtcg catcatcggg tgccgtcaaa
     2281 ctggaaatgc gccgaggcgg ccgctgaccg cggcgggtcc gtcggggtcg tggcgtgctc
     2341 gggttccgca tgaactcgct tgcgaattgc ggccgcgagt tcggcgacgg tggggtgatc
     2401 gaacagcacg tccagcgtca gtgcgattcc gaactcggct tccacggtgg caagcacgcg
     2461 catgccccgc aacgagtcgc cgccgagatg gaagaagctg tccttgcgtc cgacgctcgg
     2521 ggcttcgagc acgtcggaga agatgcgggc cagcgcggat tcttcatggt ccgacggcgg
     2581 ctcgaacgat gcaccgctcg accgctcggc aaggcctgcg agcgcggcgc gatcgaactt
     2641 gccggtcgat ccgcgcggca ggttcgccac ctcgaagatg cgtgtcggga tcttgaaggg
     2701 cgcgagtctg gcgcgaagaa agcggcgcat ttccccttca ccgagcgacc cgcgcagcac
     2761 caccgctgcc gcaacgtcct gaccgagcgt cggatgggca atggcgaacg cggcggcctc
     2821 gatcacccgc gggtgctgca tcagtgcagc ctccacctcg gcgggagcga ccttcgcgcc
     2881 gccgcgattg atgacttcct tcaggcgacc cgccagatag atgaagcctt cgcggtcgat
     2941 gtgcccggcg tcgccggtcc ggaaccagcc atcgcggaat gccgcccggt tggcttccgc
     3001 atcgttctcg tagccatcga acagttccgg tcctctgacg acgatctccc cggactcgtc
     3061 gggtccgagg aagcggccgg actcgtcggc aacggcgatc tcgatgttca ccgcacggcc
     3121 ggccgatccc ggtggcgcct gctccggcgg aaacggcgtc tgcgcaatgg ctcccgattc
     3181 ggtcatccca tagccgacga tgaccggaac cgcgaatgcg cgtgccaggc gggcccttgc
     3241 ggcttcaggc aattccgtcg tcccggacca gatcgctctc aggaagtgct tggggcgtgg
     3301 cacgcgtcgc tcgaatgcct cgagcagctc gatatgagcg gcaggaggcg cgaagagctg
     3361 ggtcggcgcc agcgtctgca gcagatcgat gatctcgtcc gcggtcggac gcgccggaac
     3421 gatgttcgag ccgcccgtca acacgcccgc cagcaggcgg cggataccgg cgctcgagtg
     3481 caggggcagc aggatcagac aacggtcgga ttcgtcgatg cgggccagtt cgacccgatt
     3541 gcgtgcggct tccgcgacac gccattgcag ccacggcacg atcttgggcg tgccggtggt
     3601 tcccgatgtg tgcatcagca acgcgatgtc atcgagttgc ggcgggtccg attccacacg
     3661 gcggcgcaac ggcgaatgga tcggttccag tgtgctgtcg ccgtcttgcc ccgtcgcaga
     3721 ccgaagcctc agcaacggga cgccggcgcg ttgcgcggcg cgcagaacag cggcgttcgt
     3781 gtccgccgag acgatcaggg catccaagcg catggcgacg acgaggtgca ccagcgcact
     3841 ctcgtcgagc gcttcgttca agggcgcgca ggtcgcggtc gagcagacgc ccagcaacgc
     3901 gacggcgaat tcgggtccgt ccggcaacgc gacgccgacg cgcgccgtgc gcccgaagcc
     3961 ggcatcggca agtccggatg cgatcgccct caaccggcgt ccgagcctga catagctcag
     4021 aacgggtttg cctggagcga ggatggcgac ggccgccgca tggtctcgaa cggacgtcgc
     4081 gagcagctgc gcaaaggcgc ctggtggatg cctggggcga gcccagggcg gctggtcgtc
     4141 gtcgagtgcg ctcatcgagg tcgtgtcgat cgctgccgcc gtcgctgcgg agcgtcgccg
     4201 atgatagccg gccacgctgc ggtgacagct tcgcgcagac cgcccacgcc tcgggattcg
     4261 cgcccgttgg gtcggcgcag cgcgcaacgc ccggtcgtcg ctgaggtcag gccgactcgt
     4321 acagccgggt cagcacgaac tcgcgatggc cgagcgcctc cgccgcggtc aggcgaccgt
     4381 tggcggtgcg cagcatgcac tcgagcagct tgtcacccgc ctggtcgagc gtgatttcac
     4441 gctgcagcag gcccgaagtg tcgacgtcga tgtgctcgct catcagccgc acagtgcgcg
     4501 ggttggcgca gatcttgatc accggcagga tcggattgcc gatcacgttg cactggccgg
     4561 tggggaagaa atgcactgcg aatccgctgg ccgcgcatag cgtcaccatc tcggccgctg
     4621 ccgacgacga atccatgaac cacagtcccg ggtgcgtcgg catctcggcc ttgtcgagca
     4681 cgccgtcgac cgtgcatttc tttccgatct tctggatgtt gccgag
//
LOCUS       DBNHBY010000471         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1722119, whole genome shotgun sequence.
ACCESSION   DBNHBY010000471 DBNHBY010000000
VERSION     DBNHBY010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1722119"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..806)
                     /locus_tag="AC16HB_11675"
     CDS             complement(6..806)
                     /locus_tag="AC16HB_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR5607494.1"
                     /translation="MKPGPEPQSCLLVARDATAWRATFDAFAAYGVTLTFADDLASAR
                     TALHALRPAVALIDADGLGSDHIPLLRGLVAEAHVPIVALLDPADEVGQIMALEVGAT
                     DVVNRAASPRLLLARLNRLTSAITSAIAPPPQLAVGGLRLDAESREARTSDVHLGLTA
                     SEFDVLFALASHAERPVSRAAIARALGRDAGTGRAVDMAVCRIRRKLREAGVTGVRIR
                     TAYGRGYVLSVEGQAAVADPWPAVAAGARRDAPGGAPSKLFDLAALPA"
     gene            complement(803..1000)
                     /locus_tag="AC16HB_11680"
     CDS             complement(803..1000)
                     /locus_tag="AC16HB_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5607495.1"
                     /translation="MRRKRLAMARRLIERTELSRAEVAETVGFGSRSALFRQLRQQFG
                     VAGSAFRPRAAAGAWSRLEHV"
     gene            complement(1001..1768)
                     /locus_tag="AC16HB_11685"
     CDS             complement(1001..1768)
                     /locus_tag="AC16HB_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012392.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5607496.1"
                     /translation="MIIRSFISCLASTAGRPEDVARRSAILARWQRENVVISASLRDS
                     AFGPCTHRLSIKAAWGGREHYFLGNRRLTVDEDSYLILNDGAHYSSVADAAGPVASFS
                     VFFRPGLADEVLDALALPPVRALDNAGRVAKRAPYFAEHLRPHDEIVSPLLRAVQRRV
                     DAGEDDDDWYEERLQMLLARMVEAEHRFRDAALKITQVQSSTRDELHRRVRIAADYIH
                     SSYTRPITLDNIAAAAHLSKYYLVRLFAQVHGLTPHA"
     gene            2212..>3016
                     /locus_tag="AC16HB_11690"
     CDS             2212..>3016
                     /locus_tag="AC16HB_11690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5607497.1"
                     /translation="MHRTTRLTTHLTLAFGGWMALAPALTAAQQPADTQKLERVEITG
                     SSIKRIEGETALPVQVITREEIQKTGAATVEQLLQTVAAISSSGTLTASTVSSATTGG
                     ISSISLRGLTSLRTLVLVNGRRITPYGIGFTGDSVSVDVNSIPLAAVERVEILKDGAS
                     AIYGSDAIAGVVNFILRKDFKGLELTGEYGDTTDGGARLKRGSLMYGFGDLGRDRYNL
                     MMLASYQKEDPLFGRQRKFANKGFSEAAKNTTTSGNTFPANFQTLDDNGF"
BASE COUNT          514 a         1038 c         1022 g          442 t
ORIGIN      
        1 aatcgtcagg ccggcagcgc ggccagatcg aacagtttgc tgggcgcgcc gccaggcgcg
       61 tcgcgccgcg cgcccgcggc gaccgcgggc cacggatctg cgacggccgc ctggccctcg
      121 accgacagca cgtatccgcg gccataggca gtccggatcc ggacccccgt gacgccggct
      181 tcgcgcagct tgcggcggat gcggcacacc gccatgtcga ccgcgcggcc cgtgcccgcg
      241 tcgcggccaa gcgcgcgggc gatcgcagcg cggctcaccg gcctttcggc gtggctcgcc
      301 agcgcgaaca gcacatcgaa ctcgctcgca gtgagcccca gatgcacgtc gctggtgcgc
      361 gcctcacgcg attccgcgtc caggcgcaga ccgcccactg cgagctgcgg cggcggcgcg
      421 atcgccgagg tgattgccga agtcagccgg ttcagccgcg ccagcagcag tcgcggcgac
      481 gcggcgcggt tgaccacatc cgtcgccccg acttcgagcg ccatgatctg gcccacctcg
      541 tccgcgggat cgagcagcgc gacgatcggc acatgcgctt ccgcaacgag cccgcgcagc
      601 agcgggatgt gatcggagcc gagaccgtcg gcatcgatca atgccacggc cgggcgcagc
      661 gcatgcagcg ccgtgcgcgc cgacgcgaga tcgtcggcga acgtcaacgt cacgccgtag
      721 gcggcaaagg catcgaaggt ggcgcgccac gccgtcgcgt cgcgcgccac gagcaggcag
      781 gattgcggtt ccggaccggg tttcatacgt gctccaaacg tgaccacgcg ccggccgcgg
      841 cgcgcggtcg gaaggcactt cccgccacgc cgaactgctg cctcagctgg cggaagagag
      901 ccgagcgcga accgaacccc accgtctcgg ccacctccgc gcggctcagt tcggtgcgct
      961 cgatcaaccg ccgcgccatg gccagccgct tgcggcgcag ttaggcatgg ggcgtcagcc
     1021 cgtgcacctg cgcgaacagc cgcacgaggt agtacttcga cagatgcgcg gccgcggcga
     1081 tgttgtcgag cgtgatcggg cgggtgtagg aagaatggat gtaatcggcg gcgatgcgca
     1141 cgcgccgatg cagctcgtcg cgcgtcgatg actgcacctg cgtgatcttg agcgcggcgt
     1201 cgcggaatcg atgctcggcc tcgaccatgc gcgccagcag catctgcaaa cgctcctcgt
     1261 accagtcgtc gtcgtcctcg cccgcatcga cccggcgctg cacggcgcgc agcagcggcg
     1321 aaacgatttc gtcatgtggc cgcaggtgtt cggcgaagta cggtgcgcgc ttcgcgaccc
     1381 ggcccgcgtt gtcgagcgca cgcaccggcg gcagcgcaag agcgtcgaga acctcgtccg
     1441 ccagcccggg gcggaagaac acgctgaagc tcgccacggg cccggcggca tccgccacgc
     1501 tgctgtaatg ggcaccgtcg ttgaggatca ggtaactgtc ctcgtcgacc gtcaggcgcc
     1561 ggttgccgag aaagtagtgc tcgcggccgc cccatgccgc cttgatcgac agtcggtgcg
     1621 tgcagggacc aaaggcgctg tcgcgcaacg atgcgctgat gaccacgttc tcgcgctgcc
     1681 agcgggcgag gattgcgctg cggcgggcga cgtcctcggg ccggccggcc gtcgacgcca
     1741 gacaactgat gaacgatcgg atgatcaccg cagtgcccaa cgacagaggg gattgcgcaa
     1801 cgctaatgtc actgcgtgcg ggcgtcgttg caggaattgc tcggactggc gaccatgccg
     1861 tgctccagtt atatgcatat gcatgtattc ggcggtgctg ctcccgttag cgcgtcgaac
     1921 tgtcgcgcgc cgctggcggc tgcgaaacag ggatgcgacg aaaggcgccg attgccgctc
     1981 gaatcgctcc gtcggcggag cgaaccgcgc gcgcatgcgc gatcctgagc accggcgcag
     2041 tgacattgct gtcaccgtcc ggaggatgcg cgggtgttgc ccctcgacga cagccgagcg
     2101 acgcgcgcaa tcgccgcaac acggagcatc ggcacggttc ctagagtcgt cccatcgtgg
     2161 tcgccgccac gatttgcgcg ccgatcgcgc tcgaacggaa ggggaaccca catgcacagg
     2221 acgacacgtc tgacgacaca cctcacgctc gcgttcggcg gctggatggc gctggcgccg
     2281 gcgctcaccg ccgcgcagca gccagccgac acgcagaagc tggagcgcgt ggagatcacc
     2341 ggctccagca tcaagcgcat cgaaggcgag accgcgctgc cggtccaggt catcacacgc
     2401 gaggagattc agaagacggg cgccgccacg gtcgagcaac tgctgcagac ggtagcggcg
     2461 atctcgagct cggggacgct caccgcttcg acggtttcga gcgccaccac gggcggcatc
     2521 tcttcgatat cgctgcgcgg gctgacgtcg ctgcgcacgc tggtgctcgt caacggtcgt
     2581 cgcatcactc cctatggcat cggtttcacc ggcgactcgg tgtcggtcga tgtcaacagc
     2641 attccgctcg ccgcggtcga gcgcgtggag atcctgaagg acggtgcctc ggcgatctac
     2701 ggctccgacg cgatcgccgg cgtggtcaac ttcatcctgc gcaaggactt caaggggctc
     2761 gagctcaccg gcgagtacgg cgacaccacc gacggcgggg cccggttgaa gcggggctcg
     2821 ctgatgtacg gcttcggcga tctcggccgg gaccgctaca acctgatgat gctcgcgtcg
     2881 taccagaaag aggacccgct cttcggcagg cagcggaagt tcgcgaacaa gggcttcagc
     2941 gaggcggcca agaacacgac gacttcaggc aacacctttc ccgccaattt ccagacgctc
     3001 gacgacaacg gatttt
//
LOCUS       DBNHBY010000472         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1179991, whole genome shotgun sequence.
ACCESSION   DBNHBY010000472 DBNHBY010000000
VERSION     DBNHBY010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1179991"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="AC16HB_11695"
     CDS             complement(<1..1259)
                     /locus_tag="AC16HB_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5607498.1"
                     /translation="MLTHLQIRDFAIVEAVELEFARGFTVLTGETGAGKSILVDALLL
                     AVGGRADSGAVRHGTERAEVSATFDVSHNAPARAWLAEQSIEHEGEVQLRRVVGTDGR
                     GRAYLNGQAVPVQSLRALGELLVDVHGQLEFQSLSRRGEQRAALDGSGQLAELVAGVR
                     AAHETWRVLDEQRSGHQRLERERADRLELLGHYCAELAALDPKDGEAEALLDERKRIA
                     SLGRLAEGSAQLEALLAGDEGGVTSALARAQGVLRQLVALDTTLEAAASQVEEAAIAA
                     REALATLRRYADTLDADPARQEWVEARLAALEAVARKHRVDVAALLATRQALDAELAE
                     LQHGTLSAAELERRLEQARGTYLALAAQLSAARQKAAAALDAQVTAHMQSLGMPGGVF
                     VTRVAPLDPPQFSAHGNDEIEFLVSANP"
     gene            complement(1274..2182)
                     /locus_tag="AC16HB_11700"
     CDS             complement(1274..2182)
                     /locus_tag="AC16HB_11700"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HIR5607499.1"
                     /translation="MDERATRPHCVALAGRPADPRVAEAMRTLVGHLLGRGYAVRLAA
                     DETFVLTGDRIETVPEADLALDCRLVVAVGGDGTMLHAARMAAVADVPVLGVNRGRLG
                     FLADVAPDRMIESVDDALAGRCVAERRMLLAANVVADGRSVAALALNDVVVSKGETGR
                     MVDLRTWVNGVYVNTHAGDGFIIATSTGSTAYALSCSGPIMHPSLDAIVLVPICPHTL
                     SDRPIVVPAGSVVEIELADRFEARAQVVCDGMVLCELEPGVRLRIERAKVSATLLHPP
                     GHDYYRILRNKLNWGRGTREGQPPGA"
BASE COUNT          299 a          786 c          790 g          331 t
ORIGIN      
        1 gggttggcgc tgaccaggaa ttcgatttcg tcattgccgt gcgcgctgaa ctgcggtgga
       61 tcgagcggcg cgacgcgcgt gacgaacacg ccgccgggca tgccgagcga ctgcatgtgt
      121 gcggtgacct gcgcatccag cgccgccgcc gccttctggc gtgccgcgct gagctgcgca
      181 gcgagcgcga ggtaggtccc ccgggcctgc tccaggcgcc gttccagctc ggctgcgctc
      241 agcgtgccgt gctgcagctc ggcgagttcg gcgtccagcg cctgccgcgt cgccagcagc
      301 gcggccacgt caacccgatg cttgcgcgcg accgcctcga gcgctgcgag gcgcgcttcg
      361 acccattcct ggcgcgctgg atcggcatcg agcgtgtccg catagcgccg cagcgtggcg
      421 agtgcttcgc gcgcggcgat ggcggcttcc tcgacctggc tcgcggcagc ctcgagcgtg
      481 gtgtccagcg caacgagctg gcgcagcaca ccttgcgcac gggcgagggc cgaagtgaca
      541 ccgccttcgt ccccggcgag cagtgcctcc agctgcgcgc tgccttcggc gaggcgcccg
      601 aggctcgcga tgcgtttgcg ttcgtcgagc agcgcctcgg cctcgccgtc cttcggatcg
      661 agcgcggcca gctcggcaca gtaatgcccc agcagttcga gtcggtcggc acgctcccgt
      721 tcgagccgct ggtgaccgga acgctgctcg tcgaggacgc gccaggtctc gtgcgcggcc
      781 cgcacccctg caacgagttc cgccagctgc ccgctgccgt cgagcgcggc gcgctgttcg
      841 ccacgccgtg acagtgactg gaactccagc tggccgtgca cgtccaccag cagttcgccg
      901 agcgcgcgca gcgactgcac cggcacggcc tggccgttca ggtacgcacg gccgcggccg
      961 tcggtgccga cgacgcggcg cagctggact tcgccttcgt gctcgatcga ctgctcggcg
     1021 agccaggcgc gtgcaggcgc gttgtgcgat acgtcgaacg ttgcgctgac ttcggcgcgc
     1081 tcggtgccgt gccgtacggc cccgctgtcg gcgcgtccgc ccacggccag cagcagggcg
     1141 tcgaccagga tcgacttgcc ggcaccggtc tcgcccgtca gcacggtgaa cccgcgcgcg
     1201 aactcgagtt cgacggcctc gacgatcgcg aagtcgcgga tctgcaggtg cgtcagcatc
     1261 ggatcgtggc gcctcaggcg ccggggggct ggccttcgcg cgtgccgcgg ccccagttca
     1321 acttgttgcg caggatgcgg tagtaatcgt gcccgggcgg atgcagcagc gttgcactga
     1381 ccttggcgcg ctcgatgcgc aaccggacgc cgggttcgag ttcgcacagc accatgccgt
     1441 cgcagacgac ctgggcacgt gcttcgaagc ggtcggccag ctcgatctcc accacgctgc
     1501 cggcgggcac gacgatcggg cggtccgaca gggtgtgcgg gcagatcggt accagcacga
     1561 tcgcatcgag gctcgggtgc atgatcgggc cgctgcagga cagcgcgtac gccgtggacc
     1621 cggtggacgt ggcgatgatg aagccgtcgc cggcatgggt gttgacgtac acgccgttga
     1681 cccaggtgcg caggtcgacc atgcgacccg tttcgccctt ggaaacgacc acgtcgttca
     1741 gcgcgagggc tgccaccgac cgcccgtcgg cgacgacgtt ggccgccagc agcatgcggc
     1801 gttcggccac gcaacgaccg gcgagcgcgt cgtcgacgct ctcaatcatg cgatcgggcg
     1861 ccacgtccgc taggaacccg aggcggccgc ggttgacgcc cagcaccggc acatccgcca
     1921 cggcggccat gcgcgcggcg tgcagcatgg tgccgtcgcc gccgaccgcc acgaccagcc
     1981 ggcagtcgag cgccaggtcg gcctcgggca cggtttcgat cctgtcgccc gtgagcacga
     2041 acgtctcgtc ggccgcgagg cggaccgcgt agccacgccc cagcaggtgg ccgacgaggg
     2101 tgcgcatggc ctcggcgacg cgcggatcgg ccgggcggcc ggccagggcg acgcagtgcg
     2161 ggcgtgtggc gcgttcatcc atagggtggc gagcctttta gcacgc
//
LOCUS       DBNHBY010000473         2793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1519489, whole genome shotgun sequence.
ACCESSION   DBNHBY010000473 DBNHBY010000000
VERSION     DBNHBY010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1519489"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /gene="acnA"
                     /locus_tag="AC16HB_11705"
     CDS             complement(<1..1720)
                     /gene="acnA"
                     /locus_tag="AC16HB_11705"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR5607500.1"
                     /translation="MTHNLHDTRQTFAPGKGKTATFYSLPALAKTYPSVDRLPVSVRI
                     VLESVLRNCDGKKVTEEHVRQIANWKPNADRVDEIPFVVARVVLQDFTGVPLLADLAA
                     MRSVAARTGKDPKTIEPLVPVDLVVDHSVMIDHFGSRDALDLNMKLEFQRNRERYEFM
                     KWGMQAFDTFGVVPPGFGIVHQVNLEFLARGVHQKDGVVYPDTLVGTDSHTTMINGIG
                     VVGWGVGGIEAEAGMLGQPQYFLTPDVVGFELKGRLREGVTATDLVLTVTEMLRREKV
                     VGKFVEFFGEGTASLAVPDRATIANMAPEYGATMGFFPVDAKTVDYFKGTGRTAEEIA
                     AFEAYFKAQGLFGIPKRGDIDYTKVVTLDLATVAPSLAGPKRPQDRIEIGNVKKTFTR
                     LFSNPASASGAESGFNQPADKLNQAFRTSNGIEVRNGDVLIAAITSCTNTSNPGVLLA
                     AGLLAKKAVERGLTVKKHIKTSLAPGSRIVTEYLEKTGLLPYLEKLGFAVAAYGCTTC
                     IGNAGDLTPELNEAIVKNDLVCAAVLSGNRNFEARIHPNLKANFLASPPLVVAYAIAG
                     TMLRDLM"
     gene            complement(1717..2151)
                     /locus_tag="AC16HB_11710"
     CDS             complement(1717..2151)
                     /locus_tag="AC16HB_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607501.1"
                     /translation="MIRPITTLFVALLLTGCVSTYQLTLMPRTSGTLYYGEAVESGSQ
                     ADVSITIDNKTYTGTWVYAAPDRTTGYISGGWGWRRSAIGATVTVDNPSGGEAKALLR
                     AADGSGLRCDFRGMAYGRSGGGTCQDDKGLLYDVQIRLKEKK"
     gene            complement(2182..2793)
                     /locus_tag="AC16HB_11715"
                     /pseudo
     CDS             complement(2182..2793)
                     /locus_tag="AC16HB_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487726.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
BASE COUNT          431 a          910 c          926 g          526 t
ORIGIN      
        1 tcatcaggtc cctcagcatc gtgccggcga tcgcgtaggc gaccaccagc ggcggcgagg
       61 cgaggaagtt cgccttgagg ttcggatgga tgcgcgcctc gaagttgcgg ttgccggaca
      121 gcacggccgc gcacacgagg tcgttcttga cgatcgcctc gttcagctcc ggcgtgaggt
      181 cgccggcgtt gccgatgcag gtcgtgcagc cgtacgcggc aacggcgaag ccgagcttct
      241 ccaggtacgg cagcagcccc gtcttctcca ggtactcggt cacgatgcgc gaaccgggtg
      301 ccagcgaggt cttgatgtgc ttcttcacgg tcagtccgcg ctcgacggcc ttcttcgcca
      361 gcagtccggc ggcgagcagc accccgggat tgctggtgtt ggtgcacgac gtgatcgccg
      421 cgatcagcac gtcgccgttg cgcacctcga tcccgttgct ggtccggaag gcctgattca
      481 gcttgtccgc cggctggttg aacccgctct ccgcgccaga cgccgatgcc ggattgctga
      541 acaggcgcgt gaaggtcttc ttcacgttgc cgatctcgat gcggtcctgt ggacgcttcg
      601 gcccggccaa cgaaggagcg acggtcgcga gatcgagcgt gacgaccttg gtgtagtcga
      661 tgtcgccgcg cttgggaatt ccgaacaggc cctgtgcctt gaagtacgcc tcgaacgcgg
      721 cgatctcctc ggcggtgcgt cccgtgccct tgaagtagtc gaccgtcttc gcatcgaccg
      781 ggaagaagcc catcgtcgcg ccgtattccg gcgccatgtt cgcgatcgtc gcccgatcgg
      841 gcacggcgag cgaggccgtg ccttcgccga agaactcgac gaacttgccg accaccttct
      901 cccggcgcag catctcggtg acggtaagga cgagatcggt cgcggtcacg ccttcgcgca
      961 gccggccttt cagttcgaag ccgaccacgt ccggggtcag gaagtactgc ggctggccga
     1021 gcatgccggc ctcggcctcg atgccgccca ccccccagcc gaccacaccg atgccgttga
     1081 tcatcgtcgt gtggctgtcg gtgccgacca gcgtgtcggg atagacgaca ccgtccttct
     1141 ggtgcacgcc gcgcgccagg aattcgaggt tgacctggtg cacgatgccg aaccccggcg
     1201 gcacgacgcc gaacgtgtcg aacgcctgca tgccccactt catgaactcg tagcgctcgc
     1261 gattgcgctg gaactcgagc ttcatgttga gatccagtgc gtcgcggctg ccgaagtgat
     1321 cgatcatcac cgagtggtcg accacgaggt cgaccggcac cagcggctcg atcgtcttcg
     1381 ggtccttgcc ggtgcgtgcg gcgaccgagc gcatcgcggc caggtccgcc agcagcggca
     1441 cgccggtgaa atcctgcagc accacgcgcg caacgacgaa cggaatctcg tcgacgcggt
     1501 ccgcgttcgg tttccagttg gcgatctgcc gcacgtgttc ttccgtcact ttcttgccgt
     1561 cgcagttgcg cagcaccgat tcgagcacga tgcgaaccga caccggcagg cggtcgacgc
     1621 tcggataggt cttcgccagc gccggcaggc tgtagaacgt ggcggtcttg cctttgcccg
     1681 gcgcaaaggt ctggcgtgtg tcgtgcaggt tgtgggtcac tttttctcct tcaagcgaat
     1741 ctggacgtcg taaagcagtc ccttgtcatc ctggcaggtc ccgccgccac tgcggccgta
     1801 ggccatgccc ctgaaatcgc agcgcaggcc ggatccgtcc gcggcccgca acagcgcctt
     1861 ggcttcgccg ccgctcggat tgtccaccgt cactgtggct ccgatggcgg agcgacgcca
     1921 tccccaaccg ccgctgatgt agccggtggt tcgatccggc gccgcataca cccaggttcc
     1981 ggtgtacgtc ttgttgtcga tcgtgatgct gacgtcggct tgcgagccgc tttcgaccgc
     2041 ttcgccgtag tagagcgtgc cgctcgtgcg cggcatcagc gtcagctggt aggtcgagac
     2101 acagccggtg agcaggagcg cgacaaagag cgttgtgatc gggcgaatca tggtgtcctc
     2161 acgggagatg ctccgaactg ctcagatcac catcatgtcg acaaattcgt gaacaggtgt
     2221 taccgcgagg cgcctggcgt cgaggcagcg atcgaggatc gcgctggcct gcttcggcgg
     2281 gaacacacgc gcgagattgg tgcggaactt ctcgagcagc cgtggcagtc cctccttgcg
     2341 gcggcgccgg tggccgagcg ggtactcgac gacgaactct cccagcgcgc ggccgtccgc
     2401 cagctcgacc ttcagcgcgt tggcgatcga gcgcttggcc gggtcgtgct agtcgcgcgt
     2461 gacctcggcg tcctcaacgc aggcgatctt cgcgcgcagg cgatcgatgc gagggtcggc
     2521 gtcgaccgtg tcctcgtagt ccgcagcggt cagccggccg aagatgatcg gcacggcgag
     2581 catgtactgg atgcagtggt cgcggtcggc ggggttgtcc agcgggccct tcttgtcgat
     2641 gatgcgcagg caggcctcgt gcgtgcgcac gctgatccgg tgaatgtcgg cgtcggtctt
     2701 gccgattcgg cgcagttcgg cgtggatcgc gagcgctgcc tcgaccgcgg tctgcgcatg
     2761 gaactcggcc gggaaggcga tcttgaacag cac
//
LOCUS       DBNHBY010000474        13666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1045176, whole genome shotgun sequence.
ACCESSION   DBNHBY010000474 DBNHBY010000000
VERSION     DBNHBY010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1045176"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /gene="lpxB"
                     /locus_tag="AC16HB_11720"
     CDS             complement(<1..964)
                     /gene="lpxB"
                     /locus_tag="AC16HB_11720"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR5607502.1"
                     /translation="MAGAFLGFAAGEASGDLLAAPVLEALAQRLPQPRFAGIAGDRMA
                     AAGCEPWHHIRELSVRGYAEVLGALPRLLRMRARLRARFERERPAVFVGVDSPDFNLR
                     LEMQLRAAGIPTVQYVSPSIWAWRGGRIEKIRRAADRVLLVFPFEQKIYDDAGVAATY
                     VGHPLAAQIPLLPDVARARARLGVAAGPCIAVLPGSRRAEVDHIGPVFADAIEAMAAR
                     EPSLTFLIPAADARLRRRIDALLATRMAARPRTRVVDGESHACLEAADVVLVASGTAT
                     LEAALYKKPMVIAYRMPRVSAWIMRGIGGYLPYVGLPNILAGEFV"
     gene            complement(971..1756)
                     /gene="lpxA"
                     /locus_tag="AC16HB_11725"
     CDS             complement(971..1756)
                     /gene="lpxA"
                     /locus_tag="AC16HB_11725"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR5607503.1"
                     /translation="MARIHPSAIVDPKAELGDVEVGPFALIGPHVKIGGGTWIGAYTV
                     VEGDTTIGRRNRIYSHCALGGAPQDKKYAGERTRLVIGDDNTIREYCTFNAGTVQDQG
                     ITKIGSNNWIMAYAHIAHDCVVGDHTIFANLAQIAGHVQVGDWAILGGMTGVHQFVRI
                     GAHAMTGGGTTLLQDLPPFVICNGNPAAAHGINVEGLRRRGFAPDAITALRRAYKAVY
                     KEGRTIADACQAIDDLASEIGSAAPHLAALREFLQASKRGIVR"
     gene            complement(1756..2211)
                     /gene="fabZ"
                     /locus_tag="AC16HB_11730"
     CDS             complement(1756..2211)
                     /gene="fabZ"
                     /locus_tag="AC16HB_11730"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR5607504.1"
                     /translation="MDIHEILRHLPHRYPIILVDRVLELEAGKRIVALKNVTINEPVF
                     MGHFPHYPVMPGVLIIEAMAQAAAILSFITFDKRADADGLYYFAGIDGARFKRPVEPG
                     DQLRLEVELVREMRGVGKFSGRALVDGQVAAEADLLCAYRRIDRGAGGP"
     gene            complement(2211..3287)
                     /gene="lpxD"
                     /locus_tag="AC16HB_11735"
     CDS             complement(2211..3287)
                     /gene="lpxD"
                     /locus_tag="AC16HB_11735"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR5607505.1"
                     /translation="MAGGLTVAQLADVIERRTQARLTARILGDGTRAIDSAAPLETAQ
                     PDQLAFLANRRYRAAAAATHAGAIVLAPADRQLIFPASPAAITLIECAAPYAWFAFAA
                     QELAPTSPPTAGIDRTAAVDAGATVDASAYIGPHTTVEADAMIGARAQVGAGSFIGRG
                     ARIGADTRILPGARVYADCVIGARCIVHSGAVIGSDGFGFAPFDGAWVKIPQTGRAVI
                     GDDVEIGANTTIDRGAMGDTVIEDGCKLDNQIQVAHNCRIGAHTVIAGCVGIAGSANI
                     GRGCQLGGAAMIAGHLSIADGCVIGGGALVSRSITAPGHYSGAFPLMPHRAWERNAAL
                     VRHLDELRDRIRALERQLETKGSS"
     gene            complement(3292..3810)
                     /locus_tag="AC16HB_11740"
     CDS             complement(3292..3810)
                     /locus_tag="AC16HB_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR5607506.1"
                     /translation="MKRFSRTVTRLLALALLAAVAGAPLAAQELRVGYVNSERILRDS
                     NPAKAATAKLEQEFSKRDKELQDMGAKLKATADRFEKDAPVLAESERARRQRELTELD
                     RDFQRRQREFREDLNQRRNEELQSLLERAQRIVRQIAEQEKYDLIVQDAVYFSSRVDI
                     TDKVLRALNNGK"
     gene            complement(3882..6233)
                     /gene="bamA"
                     /locus_tag="AC16HB_11745"
     CDS             complement(3882..6233)
                     /gene="bamA"
                     /locus_tag="AC16HB_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR5607507.1"
                     /translation="MSRGCCSEASSILNRASRLPRRATRGAAFSLRAASFAVLAACSV
                     AATGQEFTVRDIRVEGIQRTEAGTVFSYLPLRVGDRYDPEKGQAAIRALFATGLFKDV
                     RLEVDGDVLVVVVEERPSIAAVELTGIKEFDKDTVKKSLREVGLAEARVLDRSLLERA
                     EQELKRQYLSRGRYDVKVTTTVTPIERNRVNVTIAVDESEAASIRAIRFTGNKAFTDG
                     QLRSEMELSTPGWFTWYTKRDQYSRQKLTGDLETLRSFYLNRGYLDFNIESTQVSISE
                     NKQDIYVTINLVEGERYTVSDIKLAGELLGLDNELRALIDVKPGDTFNAERLNVVSKR
                     ITDRLGALGYAFASANPVPEANRDKREVAFTLFVDPGRRVYVRRVNVTGNARTRDDVI
                     RREVRQFESAWYDAEKVRLSRDRIDRLGYFEKVEVETPAVAGSIDQVDINFSVKERPT
                     GSVQAGAGFSSTEKLVLQASYTQQNVLGTGHAISFEVNTSRATRTLAVTHVDPYVTLD
                     GVSRTIEVYDRKSDLAKLNLGSVDLAQRGGAVRFGVPFTEFDTIYFGLGYEGTRITLT
                     DLSPFRYIDYVNRFGKSPDALIGTIGWSRDSRDNLLVPTRGRYQRAFLEAALPVLDLE
                     YYRATYQYQQFLPLTSRFTLAFNGEVGVGGAYGGKSYPLFKNFYGGGIGSVRGFEGGS
                     LGPRDANGQALGGTRRINGSIEALAPLPGADRTLRALAFVDAGQVWGEGQQIRFGDLR
                     ASAGLGIAWISPIGPMKISFAFPIRKEAIDRLQRFQFQIGTGF"
     gene            complement(6211..7554)
                     /gene="rseP"
                     /locus_tag="AC16HB_11750"
     CDS             complement(6211..7554)
                     /gene="rseP"
                     /locus_tag="AC16HB_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR5607508.1"
                     /translation="MALLSTIFAFIFTLGVLIIVHEYGHFWVARRCGVKVLRFSIGFG
                     RPLARRMRGETEWVLAAVPLGGYVRMLDERDTDLGPIAAADLKRAFNRQSIAKRIAIV
                     LAGPAANFLLAIGVYWALNVIGVTEPRPVVAAPALGTPAERAGFRAGDVVRTIDSEAV
                     RSWNDVNWHVLRAAVDRRALDVAVETNGVSRELRLDLSQLARTDLEANPLARAGLSLY
                     GGPPRLGRIAEGGAAEQAGLKQGDRVLEIDRRPLSSARALIERVRGSPEVALEFLIER
                     DGAQRSVSVSPARVTDKDGKPIGRIGAEISDRPEMIEVLYGPLESIGMALTRTWETSV
                     FSLHMLWKMVSGEASWKNLSGPITIADYAGQTARIGSAAFLRFIALVSISLGVLNLLP
                     IPMLDGGHLLYYLIEILKGSPPADWVVEWGHRAGIGVLVLLTALALYNDVTRLLF"
     gene            complement(7563..8741)
                     /locus_tag="AC16HB_11755"
     CDS             complement(7563..8741)
                     /locus_tag="AC16HB_11755"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR5607509.1"
                     /translation="MKRVTILGATGSIGASSLDVVARHPERFAVHALTAQGKVDALAD
                     LAERFRPRVAVIADTAKESKLREALRARGLATQARAGAEALAQVAAEAGGDTVIAAIV
                     GAAGLLPTMAAARAGKRVLLANKEALVCAGALLLDAVKRGGAELLPLDSEHSALHQCL
                     AGAHRGARDVRRLILTASGGPFRTRRDLSQVTPDEACAHPNWVMGRKISVDSATLMNK
                     GLEVIEANWLFEIGPDRIEVVVHPQSVVHSMVEFADASVVAQCGTPDMRTPIAYALAY
                     PERIDSGSAALDFKTLGALTFEAPDRARFPCLGLAYAALASGGGAPAVLNAANEVAVE
                     AFLAQKLRFTRIAGVIEETLARAERSAPSDIDDVLAIDYDARRMAGEIVRRHAAVADA
                     "
     gene            complement(8738..9568)
                     /locus_tag="AC16HB_11760"
     CDS             complement(8738..9568)
                     /locus_tag="AC16HB_11760"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5607510.1"
                     /translation="MLKQRVITAVVLLALLAAALAAGSTAFAAALSFLLGAGAFEWLR
                     LAQHTAGTALAAAILFGLALFAVELAHVATPAVVVPASAAALLVWFGIAGLLVHAQRD
                     SVRIQRAASTLLCFVLLGVAAIGALDLMRHGITLLLSALAIVWVADTAAYFVGRQWGR
                     RKLASRISPGKTWAGVGGALVAVLGLAVVLAFSAPGWPVFTTLLLQRLPLTLAVIALC
                     ALVVLSIVGDLFESLLKRQVGAKDSGTTLPGHGGVLDRIDALLPVIPAAALLAEKALP
                     "
     gene            complement(9575..10327)
                     /gene="uppS"
                     /locus_tag="AC16HB_11765"
     CDS             complement(9575..10327)
                     /gene="uppS"
                     /locus_tag="AC16HB_11765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR5607511.1"
                     /translation="MASTVQAAQRPAIPRHVAIIMDGNGRWATARHLPRVAGHSRGVD
                     AVRHAIEACGRRGVQYLTLFAFSSENWKRPQDEVSTLMRLFVNALEKEVDKLNDNGVR
                     LRVVGDMSAFDAGLQALIADAQRRTAGNDKLHLTVCASYGGRWDITQAVRSLLRAQPE
                     LAAHPERIDESLLASHLVLAFAPEPDLLIRTGGERRVSNFLLWHLAYSELYFSELLWP
                     DFDTTALDAALAWFATRERRFGRVSAQLALAT"
     gene            complement(10337..10897)
                     /gene="frr"
                     /locus_tag="AC16HB_11770"
     CDS             complement(10337..10897)
                     /gene="frr"
                     /locus_tag="AC16HB_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5607512.1"
                     /translation="MNIAEIRKSTEQKMHKSVEALKHDLTKIRTGRAHTGLLDHIHVD
                     YYGAQVPLAQVANVGLGDARTITIQPWEKKMVQPIEKAIRDSDLGLNPSTSGDVIRVP
                     MPPLTEERRKELTRVVRHEGENARVAVRNLRRDAITHLKELLKKKEVAEDEERRAQDE
                     IQKLTDRHIVDVDKLVAEKEKEIMTV"
     gene            complement(10923..11639)
                     /gene="pyrH"
                     /locus_tag="AC16HB_11775"
     CDS             complement(10923..11639)
                     /gene="pyrH"
                     /locus_tag="AC16HB_11775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5607513.1"
                     /translation="MATPAHKRVLLKLSGEALMGDDPFGINRETIEGMVADISEVVKL
                     GVELAIVIGGGNIFRGVALGAEGMDRATADYMGMMATVMNAMALQDAMRRAGIEARVQ
                     SALNIEQVVEPYIRPKAIRYLEEGKVVIFAAGTGNPFFTTDTAAALRGSEMGVEVVLK
                     ATKVDGVYSADPKKDRKATRYATISFDEALAKRLQIMDATAFALCRDQRLPIKVFNIN
                     KPGALKRVVMGEDEGTLVHV"
     gene            complement(11652..12545)
                     /gene="tsf"
                     /locus_tag="AC16HB_11780"
     CDS             complement(11652..12545)
                     /gene="tsf"
                     /locus_tag="AC16HB_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5607514.1"
                     /translation="MAEITAGMVKELREKTDAPMMECKKALAEAGGDMAKAEEILRVK
                     LGSKASKAASRITAEGMVAVHVDAAGALAAMVEVNCETDFVAKNDDFQAFVRKLAALV
                     AEKNPADIAALSALPYAGGTVEATRTALIGKIGENMSIRRFARTAAKGRIASYVHGGS
                     KIGVLVDVTGGDADLGKDLAMHIAASKPKSLDASGVPQELVDAERRIAIEKAREAGKP
                     EAMLEKIAEGSVQKFLKESTLLGQVFVKAADGRQTVEQLLQSKGAGVVGFVLYVVGEG
                     IEKKQSDFAAEVAAQAAAARA"
     gene            complement(12654..13403)
                     /gene="rpsB"
                     /locus_tag="AC16HB_11785"
     CDS             complement(12654..13403)
                     /gene="rpsB"
                     /locus_tag="AC16HB_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5607515.1"
                     /translation="MSVTMREMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLER
                     TLENFLQASKFVRQLAANRGTILFVGTKRQAREIVVEEAKRCGMPCVDQRWLGGMLTN
                     FKTIKASIKRLKEMEQTIEEGGLERMSKKEALTFQREVDKLNKAIGGIKDMTALPDAL
                     FVVDVGYHKIAVAEAAKLGIPVVAVVDTNHSPDGIAYVIPGNDDSSRAVRLYTRGVAD
                     AVLEGKAASLQGVVQATTGDEFVEVEEEDAA"
BASE COUNT         2158 a         4792 c         4433 g         2283 t
ORIGIN      
        1 cgacgaattc gcccgcaagt atgttcggca gcccaacata aggcaggtag ccgccgatgc
       61 cccgcatgat ccacgccgac acgcgcggca tgcggtacgc gatcaccatc ggctttttgt
      121 acaaggccgc ttccagcgtc gcggtgccgc tggcaaccag cactacatcg gctgcttcca
      181 ggcaggcgtg cgactcgccg tcgaccacgc gcgtgcgcgg gcgcgcggcc atgcgtgtcg
      241 ccagcaacgc gtcaatgcgc ctgcgcagcc gcgcatccgc ggcgggaatg agaaaggtca
      301 ggctcggctc gcgcgcagcc atcgcctcga tcgcatcggc gaacaccgga ccgatgtgat
      361 cgacctcggc gcgccggctg ccgggcagta cggcgatgca cgggccggcc gccaccccga
      421 gccgcgcgcg cgcccgcgcg acatcgggca ggagcggaat ctgcgccgcc agcgggtgcc
      481 cgacgtaggt cgcagcgacc ccggcgtcgt cgtagatctt ctgctcgaac gggaacacga
      541 gcagcacgcg gtcggctgca cgccggattt tttcgatgcg gccgccgcgc caggcccaga
      601 tcgacgggct gacgtattgc acggtcggaa tgccggcggc gcgcaactgc atttcgaggc
      661 gcaggttgaa gtcgggcgag tcaacgccga cgaacacggc cgggcgttcg cgctcgaagc
      721 gcgcgcgcaa tcgcgcgcgc atgcgcagca gtcgcggcag cgcgccgagc acttcggcgt
      781 agccgcgcac cgacagttcc ctgatgtgat gccatggctc gcagcccgcc gctgccatgc
      841 ggtcccccgc gatgccggca aagcgcggct gcggcaggcg ttgcgcaagc gcctccagca
      901 ccggcgcggc cagcaggtct ccggaggcct ctcccgcggc gaagccgagg aacgcgcccg
      961 ccatcgtgac tcagcgcacg ataccgcgct tggatgcctg aagaaattcg cgcaacgcgg
     1021 ccagatgcgg cgcggctgac ccgatctcgg acgctaggtc gtcgatcgcc tggcacgcgt
     1081 ccgcgatcgt ccggccttcc ttgtataccg ccttgtaggc gcggcgcagc gcagtgatcg
     1141 catccggcgc gaagccgcgc cgccgcaacc cctcgacgtt gattccgtgc gcggccgccg
     1201 gattgccgtt gcagatcacg aacgggggca ggtcctgcag cagcgtcgtg ccgccaccgg
     1261 tcatcgcgtg cgcgccgatg cggacgaact ggtgcacgcc ggtcattccg cccaggatcg
     1321 cccagtcgcc cacctgaacg tggcccgcga tctgcgcaag attggcgaag atcgtgtgat
     1381 cgccgaccac gcaatcgtgc gcgatatgcg cgtaggccat gatccagttg ttcgagccga
     1441 tcttcgtgat tccctggtcc tgcaccgtgc ctgcattgaa cgtgcagtac tcccggatcg
     1501 tgttgtcgtc gccgatcacg agccgggtcc gctcgcccgc atacttcttg tcctgcggtg
     1561 cgccgccgag tgcgcagtgc gagtagatcc ggttgcgccg gccgatcgtc gtgtcgcctt
     1621 cgaccaccgt gtacgcgccg atccaggtgc cgccaccgat cttcacgtgc ggaccgatca
     1681 gcgcgaacgg gccgacctcg acgtcgccca gttccgcctt cggatcgacg atagcgctcg
     1741 gatggatgcg cgccatcacg ggcccccggc gccgcggtcg atcctgcggt acgcgcacag
     1801 caggtccgcc tcggccgcga cctggccgtc gaccagcgcg cggccgctga acttgccgac
     1861 gccgcgcatc tcgcgcacca gctcgacttc cagccgcaat tgatcccccg gctccaccgg
     1921 tcgcttgaaa cgtgcgccgt cgatgccggc gaagtagtag agcccgtcgg cgtcggcgcg
     1981 cttgtcgaac gtgatgaacg acaggatcgc cgccgcctgc gccatcgctt cgatgatcag
     2041 taccccgggc atcaccggat agtgcggaaa gtgacccatg aacacaggct cgttgatcgt
     2101 cacgttcttc agcgcgacga tgcgcttgcc ggcctccagc tcgagcacgc ggtcgaccag
     2161 gatgatcggg tagcggtgcg gcagatggcg caggatttcg tggatgtcca tcacgacgag
     2221 cccttcgttt cgagttgccg ttcgagagcg cggatgcggt cacgcagctc gtccaggtga
     2281 cgcaccagcg cggcgttgcg ttcccacgcg cggtgcggca tcagcggaaa ggcgccgctg
     2341 taatggcccg gcgcggtgat gctgcgcgag accagcgcac cgccgccgat cacgcagccg
     2401 tcggcgatcg acaggtggcc ggcaatcatc gccgcaccgc cgagctggca gccgcggccg
     2461 atatttgcgc tgccggcgat gccgacacag ccggcgatca ccgtgtgcgc gccgatgcgg
     2521 cagttgtgcg cgacctggat ctgattgtcg agcttgcacc cgtcctcgat cacggtgtcg
     2581 cccatcgcgc cgcgatcgat cgtcgtgttg gcgccgatct ccacgtcatc gccgatcacg
     2641 gcccggcccg tctgcggaat cttgacccat gcgccatcga acggcgcaaa accgaagccg
     2701 tcggagccga tcaccgcgcc gctgtgaacg atgcagcgtg cgccaatcac gcagtcagcg
     2761 tagacgcgcg cgcccggcag gatgcgcgtg tcggcgccga tccgcgcgcc gcggccgatg
     2821 aagctgccgg cgccgacctg tgcccgcgcg ccgatcatcg cgtcggcctc gaccgtcgtg
     2881 tgcggcccga tgtatgcgct ggcatcgacc gtcgcgcctg catcgaccgc agcggttcga
     2941 tcgataccgg ccgttggcgg cgacgtcggt gcgagttcct gggcggcgaa ggcgaaccac
     3001 gcgtagggcg ccgcgcattc gatcagcgtg atggcggcag gcgacgcggg aaagatcagc
     3061 tgccggtcag ccggcgccag cacgatcgcg ccggcgtgcg tcgccgccgc ggccgcgcga
     3121 tagcggcggt tggcgagaaa ggcgagttgg tcgggctggg cggtttcgag cggtgccgcg
     3181 ctatcaatcg cgcgcgtgcc gtcgccgagg attctggccg tcagtcgcgc ctgggtccga
     3241 cgctcgatga cgtcggccag ctgcgccacc gtgaggccgc cggccatcgc gctacttgcc
     3301 gttgttcagc gcccggagca ccttgtcggt gatgtccacc cgcgagctga agtacacggc
     3361 gtcctgcacg atcaggtcgt acttctcctg ctcggcgatc tggcgcacga tgcgctgcgc
     3421 gcgctcgagc agcgattgca gttcctcgtt gcgacgctgg ttcaggtcct cgcggaattc
     3481 gcgctggcgg cgctggaagt cgcggtccag ttcggtcagt tcgcgctggc ggcgggcgcg
     3541 ctcgctctcg gccagcaccg gcgcgtcctt ctcgaagcga tcggccgtcg ccttcagctt
     3601 cgcgcccatg tcctgcagtt ccttgtcgcg cttggagaac tcctgttcca gcttggcggt
     3661 cgcggccttg gccggattcg aatcgcgcag gatgcgctcc gaattcacgt agccgaccct
     3721 cagctcctgg gcggccagcg gcgcgccggc gaccgcagcc agcagcgcta gcgccagcag
     3781 acgagtcacg gtgcgggaaa atcgtttcat gcgcgcccct gatgcgcaaa aggtgtcgaa
     3841 ttctgccaca gcgatcgccg ccgacgcggc gcgacgccgt ctcaaaagcc ggtgccgatc
     3901 tggaactgga agcgctgcag ccggtcgatc gcttccttgc gaatcgggaa cgcgaagctg
     3961 atcttcatcg gcccgatcgg cgagatccag gcgatgccga ggcccgcgga cgcgcgcagg
     4021 tcgccgaatc ggatctgctg tccctcgccc cagacctggc cggcatccac gaatgcgagc
     4081 gcgcgcagcg tgcggtcggc gccgggcagc ggcgccagcg cctcgatcga gccgttgatc
     4141 cgccgcgtgc cgccgagtgc ctggccgttg gcatcgcgcg gcccgagcga tcccccctcg
     4201 aagccgcgca cgctgccgat gccgccgccg tagaagttct tgaacagcgg atacgacttg
     4261 ccgccgtagg cgccgcccac gccgacctcg ccgttgaacg ccagcgtgaa gcggctcgtc
     4321 agcggcagga actgctggta ctggtacgtc gcgcggtagt actcgaggtc gagcaccggc
     4381 agcgccgcct cgaggaacgc gcgctggtag cggccgcgcg tcggcaccag caggttgtcg
     4441 cggctgtcac gggaccagcc gatcgtgccg atcagcgcgt ccgggctctt gccgaagcgg
     4501 ttgacgtagt cgatgtaacg gaacggcgag aggtcggtca gcgtgatgcg cgtgccctcg
     4561 taaccgaggc cgaagtagat cgtgtcgaac tcggtgaacg gcacgccgaa acggaccgcg
     4621 ccgccacgct gcgcgagatc gacgctgccg aggttcagct ttgcaaggtc cgacttgcgg
     4681 tcgtagacct cgatggtgcg gctgacgccg tcgagcgtca cgtaggggtc gacgtgcgtc
     4741 acggcgagcg tgcgggtggc gcggctggtg ttgacctcga acgagatcgc gtggccggtg
     4801 ccgagcacgt tctgctgcgt gtagctcgcc tgcagcacga gcttctcggt gctggagaag
     4861 ccggcgccgg cctggacgct gcccgtcggc cgctccttca ctgagaagtt gatgtcgacc
     4921 tggtcgatcg atcccgcgac cgcgggcgtt tcgacctcga ccttctcgaa atatccgagg
     4981 cggtcgatgc ggtcgcgcga cagccgcacc ttctccgcgt cgtaccaggc gctctcgaac
     5041 tggcgcacct cgcgccggat gacgtcgtcg cgcgtgcgcg cgttgccggt gacgttcacg
     5101 cgtcgcacat agacgcgccg gccgggatca acgaacagcg tgaacgcgac ctcgcgtttg
     5161 tcgcggttgg cctccggcac cgggttggcg gacgcgaagg cgtagccgag cgcaccgagc
     5221 cggtcggtga tgcgcttgga gaccacgttc agcctttcgg cattgaaggt gtcgcccggc
     5281 ttcacgtcga tcagcgcgcg caactcgttg tcgagcccca gcaactcgcc cgcgagcttg
     5341 atgtcggaaa ccgtgtagcg ctcgccctcg accaggttga tcgtgacgta gatgtcctgc
     5401 ttgttctcgc tgatcgagac ctgtgtcgat tcgatgttga agtcgaggta gccgcggttc
     5461 aggtagaagc tgcgcaaagt ctcgaggtca ccggtgagct tctggcgcga atactggtcg
     5521 cgcttcgtgt accaggtgaa ccagcccggc gtggacagct ccatttccga ccgcagctgg
     5581 ccgtcggtga acgccttgtt gcccgtgaag cggatcgccc ggatgctcgc cgcctcgctc
     5641 tcgtcgaccg cgatggtgac attgacgcgg ttgcgctcga tcggcgtcac cgtggtcgtg
     5701 accttcacgt cgtatcgacc gcggctcagg tactgccgct tcagttcctg ctcggcgcgc
     5761 tccagcaggg agcggtcgag cacgcgtgcc tcggccagtc cgacctcgcg cagcgacttc
     5821 ttgaccgtgt ccttgtcgaa ctccttgatg ccggtgagct cgactgccgc gatcgacggc
     5881 cgctcctcga cgaccacgac cagaacgtcg ccatcgacct cgaggcgcac gtccttgaag
     5941 agcccggtgg cgaacagcgc gcggatcgcg gcctgcccct tttcagggtc gtaacggtcg
     6001 ccgacgcgca gcggcaggta gctgaacacg gtgcctgcct cggtgcgttg aatgccttcg
     6061 acgcggatgt cgcgcaccgt gaactcctgc ccggtggcgg cgacgctgca ggcggccagc
     6121 acggcaaagc tggcggcacg caacgagaag gcagcgccgc gagtcgcgcg gcgcggcaaa
     6181 cgggatgctc tgttcaagat cgaggaggct tcagaacagc agccgcgtga catcgttgta
     6241 gagcgccagc gcggtgagga ggacgagcac gccgatgccg gcgcgatgac cccattcaac
     6301 gacccagtcg gccggcgggc tgcctttgag gatttcgatc agatagtaca gcaagtgtcc
     6361 gccatcgagc atcggaatcg gcaacaggtt cagcacaccg agactgatag aaaccagcgc
     6421 aatgaacctc agaaacgccg cgctgccgat ccgcgcggtc tgccccgcat agtctgcgat
     6481 cgtgatcggc ccggagaggt tcttccacga cgcctcgccg ctcaccatct tccacagcat
     6541 gtgcagcgag aacaccgagg tctcccaggt gcgcgtcagc gccatgccga tgctttcgag
     6601 cggaccgtag agcacctcga tcatctcggg ccggtcggag atctcggcgc cgatgcggcc
     6661 aatcggcttg ccgtccttgt cggtcacgcg cgccggactc acgctgacgc tgcgctgcgc
     6721 gccgtcgcgc tcgatcagga actcgagtgc gacctcgggc gaaccgcgca ctcgttcgat
     6781 cagcgcgcgc gccgacgaca ggggccgccg gtcgatctcg agcacgcgat cgccctgctt
     6841 cagccccgcc tgttcggccg cgccaccctc ggcaatgcga cccaggcgcg gcggcccgcc
     6901 atagagcgac agccccgcgc gtgccagcgg gttcgcttcc agatcggtgc gcgccaactg
     6961 cgacagatcg agccgcagct cgcgtgaaac accgttcgtc tcgaccgcta cgtcgagcgc
     7021 cctgcggtcg accgcagcgc gcagcacgtg ccagttgacg tcgttccacg aacgcaccgc
     7081 ttcgctgtcg atcgtgcgca cgacgtcgcc ggcacgaaag ccggcgcgct ccgcgggcgt
     7141 gccgagcgcg ggcgcggcaa cgaccggccg cggttcggtc acgccgatca cgttcagcgc
     7201 ccagtacacg ccgatcgcga gcaggaaatt ggccgccggc cccgccagca cgatcgcgat
     7261 gcgcttggcg atcgactgcc gattgaacgc acgcttcagg tccgcggccg cgatcggtcc
     7321 gaggtcggtg tcgcgctcgt cgagcatgcg cacatagccg ccgagcggca ccgcggccag
     7381 cacccactcg gtttcgccgc gcatgcgccg cgcgagcggt cggccgaagc caatcgaaaa
     7441 gcgcaggacc ttgacaccac agcggcgtgc aacccagaag tggccgtact cgtgcacgat
     7501 gatcagcacg ccgagcgtga agatgaaggc gaagatcgtt gaaagcagcg ccacgcgtcc
     7561 tcctatgcgt cggcgacggc cgcatgccgc cgcacgattt cgcccgccat gcggcgcgcg
     7621 tcgtagtcga ttgcgagcac gtcgtcgatg tcgctcggcg cgctgcgctc tgcacgcgcc
     7681 agcgtttcct cgatcacgcc ggcaatgcgc gtgaaccgca gcttctgcgc gaggaaagct
     7741 tcgaccgcga cttcgttggc ggcgttcagc accgcgggcg cgccgccgcc gctcgccaga
     7801 gccgcgtacg caagcccaag gcacgggaag cgcgcacgat cgggggcttc gaacgtcagc
     7861 gcgccgagcg tcttgaagtc cagcgccgcc gaaccgctgt cgatgcgctc cgggtatgcg
     7921 agcgcatagg cgatcggcgt gcgcatatcg ggcgtgccgc actgggcgac caccgacgca
     7981 tcggcgaatt cgaccatcga gtgcaccacg ctctgcgggt gcacgacgac ctcgatgcgg
     8041 tcggggccga tctcgaacag ccaattggct tcgatcacct cgagtccctt gttcatcagc
     8101 gtcgccgagt cgaccgagat cttgcggccc atcacccagt tcggatgcgc acaagcctcg
     8161 tccggggtta cctgcgacag gtcgcgccgg gtgcggaacg gcccgcccga ggccgtcagg
     8221 atcagccgcc gcacgtcccg cgcgccgcgg tgggcgccag ccagacactg gtgcaaggcg
     8281 ctgtgttcgc tgtcgagcgg caacagttcc gcgccaccgc gcttgaccgc gtcgagcagc
     8341 agcgcgccgg cgcacaccag tgcttccttg ttggcgagca gcacgcgctt gccggcgcgt
     8401 gcggcggcca tcgtcggcag caagcctgcc gcgccgacga tggcggcgat caccgtgtcg
     8461 ccgccagctt cggcagcaac ctgcgccagc gcctcggcgc cggcgcgcgc ctgggtcgcg
     8521 agcccgcgcg cgcgcagcgc ttcgcgcagc ttggactcct tggcggtgtc ggcgatcacg
     8581 gcgacccgtg gccggaagcg ttcggccaga tccgcgagcg cgtcgacctt gccctgcgcg
     8641 gtcaacgcat gaaccgcaaa gcgctcgggg tgccgcgcga ccacgtcgag gctgctggcg
     8701 ccgatcgagc cggtcgcacc gaggatcgtc acgcgcttca cggcagagcc ttttcggcca
     8761 gcagcgcggc cgcggggatc accggcagca gcgcatcgat gcgatcgagc acgccaccgt
     8821 gtcccggcag cgtggtaccg ctgtccttgg caccgacctg ccgcttgagc aggctctcga
     8881 acaggtcgcc gacgatcgac agcacgacga gtgcgcacag cgcgatcacc gccagcgtca
     8941 gcggcaggcg ctgcagcagc agcgtcgtga agactggcca gcccggcgcg ctgaatgcca
     9001 acacgaccgc gagcccgagc accgcaacca gcgcgccgcc gacgccggcc caggtcttgc
     9061 ccggactgat cctcgaagcg agcttccttc ggccccactg cctgccgacg aaatacgccg
     9121 cagtatcggc gacccagacg atcgcgagcg ccgacagcag caacgtgatt ccgtgtcgca
     9181 tcaggtcgag cgcgccgatc gccgccacgc cgagcagcac aaagcacagc aaggtcgacg
     9241 ccgcacgctg gatgcgaacc gagtcgcgct gcgcatgtac cagcaggccg gcgatgccga
     9301 accagaccag cagcgccgct gccgatgccg gcacgacgac cgcgggcgtc gcgacgtgcg
     9361 ccagttcgac ggcgaacagc gcaagaccga acaagatcgc cgcggcgagc gccgtgcccg
     9421 ccgtgtgctg tgccagccgc agccattcga atgcgcccgc cccgagcagg aacgacaatg
     9481 cagcagcgaa ggcggtcgac ccggccgcca gcgccgctgc cagcagggcc agcagcacga
     9541 ccgcggtaat cacgcgctgc ttcagcatgc aggctcaagt ggcgagcgcc aactgcgcgc
     9601 tgacgcgacc gaagcggcgt tcacgcgtcg caaaccatgc gagcgcggca tcgagcgcgg
     9661 tcgtgtcgaa atcgggccag agcaactcgg aaaagtacag ttcggagtat gcgaggtgcc
     9721 acagcaggaa attgcttacg cgccgctcgc cgccggtacg gatcaacaag tccggttccg
     9781 gcgcgaacgc gagcacaaga tgcgatgcga gcaggctttc gtcgatacgt tccggatgtg
     9841 ccgccagttc gggctgcgcg cgcagcagcg aacgcaccgc ctgcgtgatg tcccagcgcc
     9901 cgccgtagct tgcacatacc gtcaggtgca gtttgtcgtt gcccgcagtg cggcgttgcg
     9961 catcggcgat cagggcctgc aggccggcat cgaacgcgct catgtcgccg acgacgcgca
    10021 gccgtacccc gttgtcgttc aacttgtcga cttccttttc gagcgcgttg acgaacagcc
    10081 gcatcagcgt actgacctcg tcctgcggac gtttccagtt ctcggagctg aacgcaaaca
    10141 gcgtcagata ctgcacgccg cgccggccgc aggcctcgat cgcgtgccgc acggcgtcta
    10201 cgccgcgcga gtgaccggcc acgcgcggca gatggcgcgc agtcgcccag cgaccattgc
    10261 catccatgat gatggccaca tggcgtggaa tcgcggggcg ttgtgcggct tgcacggtcg
    10321 aggcgatcgc gcggtctcag accgtcatga tctccttttc cttttccgcg acgagcttgt
    10381 cgacgtcgac gatgtggcgg tcggtgagct tctggatctc gtcctgggcg cgccgctcct
    10441 cgtcctcggc aacttccttc ttcttcaaga gttccttcag gtgcgtgatc gcgtcgcgcc
    10501 tcaggttgcg caccgccacc cgcgcgttct cgccttcgtg gcgcaccacc ctggtcaact
    10561 ccttgcgccg ctcctcggtc agcggcggca tcggcacccg gatcacgtcg cccgacgtcg
    10621 acggattcag cccgagatcg gagtcgcgga tcgccttctc gatcggctgc accatcttct
    10681 tttcccacgg ctggatcgtg atcgtgcggg catcgccgag gcctacgttg gcaacctgcg
    10741 ccagcggcac ctgcgcgccg tagtagtcga cgtggatgtg gtcgagcaat ccggtgtgcg
    10801 cgcggccggt gcggatcttg gtcaggtcgt gcttcagcgc ctcgaccgac ttgtgcatct
    10861 tctgctcggt ggacttgcgg atttccgcga tgttcatcgt cagcgtctcc ttcgctccta
    10921 gcctacacgt gcaccagcgt tccctcgtcc tcgcccatca caacgcgctt gagcgcgccc
    10981 ggcttgttga tgttaaagac cttgatcggc agccgctgat cacggcacag cgcaaacgcg
    11041 gtggcatcca tgatctgcag ccgcttcgcg agcgcctcgt cgaacgagat cgtcgcgtat
    11101 cgggtggctt tcctgtcctt cttcggatcg gccgagtaca cgccgtcaac cttggtggcc
    11161 ttcagcacca cttcaactcc catctccgat ccgcgcagcg cggccgcggt gtcggtggtg
    11221 aagaacgggt tgccggtgcc ggcggcgaag atcaccacct tgccttcctc caggtagcgg
    11281 atcgccttgg gccggatgta cggttcgacc acctgctcga tgttcagcgc cgactgcacg
    11341 cgcgcctcga tgccggcgcg gcgcatcgcg tcctgcagcg ccatcgcatt catcaccgtg
    11401 gccatcatgc ccatgtagtc ggcagtcgct cggtccatcc cctcggcgcc gagcgcgacg
    11461 ccgcggaaga tgttgccacc gccgatcacg atcgccagct cgacgccgag cttcacgacc
    11521 tccgagatgt cggcgaccat gccctcgatc gtctcgcgat tgatgccgaa cggatcgtcg
    11581 cccatcaggg cttcacccga cagcttcagc agaacccgct tgtgcgccgg tgtcgccatc
    11641 ggcactccgc gtcaggcgcg cgctgcggcc gcttgcgcgg cgacctctgc cgcgaagtcc
    11701 gactgcttct tctcgatgcc ctcgcccacc acgtaaagca cgaagccgac gacgcccgcg
    11761 cccttggact gcagcaactg ctcgaccgtc tgcctgccgt cggccgcctt gacgaacacc
    11821 tggccgagca acgtgctctc cttcaggaat ttctgcacgc tgccttcggc gatcttttcc
    11881 agcatcgcct cgggcttgcc agcctcgcgc gccttctcga tcgcgatgcg gcgctccgcg
    11941 tcgaccaatt cctgcgggac tccgctggca tcgagcgact tgggtttgct ggccgcgatg
    12001 tgcatcgcca ggtccttgcc gaggtcggcg tcgccgccgg tgacatcgac cagcacgccg
    12061 atcttggaac cgccgtgtac atagctcgcg atccgcccct tggccgcggt gcgcgcgaag
    12121 cgccggatcg acatgttctc gccgatcttg ccgatcagcg cggtgcgggt cgcctccacc
    12181 gtgccgccag cgtacggcag cgccgacagc gcggcgatgt cggccggatt cttctcggcg
    12241 accagggccg cgagcttgcg cacgaacgcc tggaagtcgt cgttcttggc gacgaaatcg
    12301 gtttcgcaat tgacctcgac catcgccgcc agcgcgcccg ccgcatcgac atgaacggcc
    12361 accatgccct cggcggtgat gcgcgacgcg gctttcgacg ccttgctgcc gagcttcacg
    12421 cgcagaatct cttcggcctt cgccatgtcg ccgccggcct cggccagcgc cttcttgcac
    12481 tccatcatcg gcgcatcggt cttttcgcgc agttccttga ccatgcccgc ggtaatttcc
    12541 gccatcgcgt actcctgtat tcaattgatc cgatcaggta cttagaaaaa aggggcttgc
    12601 gccccctttc ccgcagaaga cgtgcgcagc ggaacgcccc gccgcggccg gactcacgcg
    12661 gcgtcttctt cttcgacctc gacgaactcg tcgcccgtcg tcgcctgcac gacgccctgc
    12721 agcgaggcgg ccttgccttc cagcaccgca tcggcgacgc cacgcgtgta cagccgcacc
    12781 gcgcggctcg agtcgtcatt gcccgggatc acatacgcga taccgtcggg cgaatgattg
    12841 gtgtcgacca cggccaccac cgggataccg agcttggccg cctccgcgac cgcgatcttg
    12901 tggtagccga cgtcgaccac gaacagcgcg tccggaagcg cggtcatgtc cttgataccg
    12961 ccgatcgcct tgttcagctt gtcgacctcg cgctggaacg tcagcgcctc cttcttcgac
    13021 atccgctcca ggccgccttc ctcgatcgtc tgctccattt ccttcaggcg cttgatcgag
    13081 gccttgatgg tcttgaaatt ggtcagcatg ccgccgagcc agcgctggtc gacgcacggc
    13141 ataccgcaac gcttcgcctc ttcgacgaca atctcgcgcg cctgccgctt ggtgccgacg
    13201 aagaggatcg ttccccggtt tgcggccaac tggcgcacga acttggacgc ctgcaggaaa
    13261 ttctccagcg tgcgctcgag attgatgatg tggatcttgt tgcgatgccc gaagatgaac
    13321 ggcgccatct tcggattcca gaagcgggtc tggtgtccga aatggacacc cgcttccagc
    13381 atttcacgca tggtgacaga catgctgatg ctccgggcca aggttggttc caacgcgcca
    13441 gccgaggccc gccggtgcgg acagccgact gtccgcgggc gggcaccttg gtcgccggcg
    13501 cgcgctattt gccttcggcg tcagggtcga tcgtcccgga tgcgccgagg cagccagtta
    13561 ttatacgggc tttccactca agtaacccgc gccaggcaag gtcgagctcg ccgatggcca
    13621 tcgtcatcaa gaacgccgcc gatatcgacg gcatgcgtgt cgccgg
//
LOCUS       DBNHBY010000475         5848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_841682, whole genome shotgun sequence.
ACCESSION   DBNHBY010000475 DBNHBY010000000
VERSION     DBNHBY010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_841682"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="AC16HB_11790"
     CDS             complement(<1..1591)
                     /locus_tag="AC16HB_11790"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR5607516.1"
                     /translation="MSKTLIIAEKPSVAADIARALGGFTKHQDYYESDEYVLAFAVGH
                     LLQLSNPVEVQRGKWSFAHLPMIPPAFEVAPIDKKAEDRLKLLARLIKRKDVRALINA
                     CDAGREGELIFRYIVQYAHAKQPIQRLWLQSMTPAAIREAFEALRSDAEMHRLEEAAR
                     SRAEADWLVGINGTRALTAFNSKDGGFFLTTVGRVQTPTLALVVEREEKIQQFEPRDY
                     WEVRATFLARAGTYEGRWFDPDFKKRDDDPEGRAERLWDKARAEAIVAACAGKSGTVS
                     EETKPSTQAPPLLFDLTSLQREANGRFGFSAKTTLALAQALYERHKVLTYPRTDSRAL
                     PEDYVKVVKKTIDAIGGNRAYAQFAKAIQKNDWVRPNKRIFDNAKVSDHFAIIPTLQE
                     PKHLTDAEGKIYDLVVKRFLAIFFPSAEYLVTTRITTVESHGFKTEGKVLVEPGWLAV
                     YGKEAQEEQASLVKVDPNERVSTEAIEAAALQTRPPARYTEATLLSAMEGAGKFIDDE
                     ELRAAMAGKGLGTPATRAATIE"
     gene            complement(1675..2133)
                     /locus_tag="AC16HB_11795"
     CDS             complement(1675..2133)
                     /locus_tag="AC16HB_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smg family protein"
                     /protein_id="HIR5607517.1"
                     /translation="MFDVLVYLYENYGALNACPDGDALTRKLAAAGFDAEEITDAIAW
                     LQGLQRVTQDSQILREPAAGATRVFAAFEAARLGTDVMSFLSCLEASGALTPAQREIV
                     VERALATEESPVPLAKLKVIVLMVLWSLQTDIGILLLEELLDDGEDRALH"
     gene            complement(2189..3286)
                     /gene="dprA"
                     /locus_tag="AC16HB_11800"
     CDS             complement(2189..3286)
                     /gene="dprA"
                     /locus_tag="AC16HB_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR5607518.1"
                     /translation="MDRIERAAWLRLSLTPDVGPRTARALLGAFGLPAAIFDASASAL
                     GRYVPQALAQTLAAPADPATEHAIASAEAWLDASESNALLTLPDAQYPQALLATSDPP
                     PLLYAIGRLELLNRPTLAIVGSRNATRQGTDTAAAFAHRLAQAGLTIASGLALGIDAA
                     AHRGALQAAAEGAADSTVAVVGTGVDVIYPASNKALTLAIRERGVVISEFALGTPGIA
                     HNFPRRNRIIAGLARGVLVVEAALRSGSLITARLAAETGREVFAVPGSIHSPMAKGCH
                     RLIKEGAKLVEEAADILSELRMPAIAAPPPAEAAPASALLDALGYDPVDLDTLAARTG
                     RDAGSLTAELLELELAQDIARLPGNRYQRLR"
     gene            complement(3342..4625)
                     /locus_tag="AC16HB_11805"
     CDS             complement(3342..4625)
                     /locus_tag="AC16HB_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5607519.1"
                     /translation="MQSRSLDVRRLRMRAALTGALIVAAAWCVSQQARAVEPASVRWP
                     VTEQQRGVADQVAQAGVPLSALAPNAPDTYTVKTGDTLWDISSIFLTTPWRWPELWGM
                     NKDQIRNPHLIYPGQMLRLVKTGDRARLELAGEGSAPGDVVRVSPRVRDLGADKQAIS
                     SIPAKVIEPFLSRPLVVTADELNKYARIVATQEGRVFLGRGDTAYARGVTDDKVRDYH
                     VFRPARPLYDPDDENRKNPIAYEAFYLGVARVAKGGEVTTLRVEESKEEIGVGDRLVP
                     VTVQPLVNYVPHRPERALNGRIISIYSGVNQAGTQSIVALNRGARDGLEVGHVMALLE
                     TGATIRDRTVDGREFVKLPDERIGELFVFRVAETISYGLVMRVTKPVKVGDRFTDPDS
                     TEADLTATGNGAAVPVARAPEASSQRRWYNPFTWF"
     gene            4864..5373
                     /gene="def"
                     /locus_tag="AC16HB_11810"
     CDS             4864..5373
                     /gene="def"
                     /locus_tag="AC16HB_11810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5607520.1"
                     /translation="MARLPILEYPDPRLKKIARPVHVFDARLRKLISDMAETMYSAPG
                     IGLAATQVDVHERVIVIDASETKDQLRVFINPEILWASEETTLCEEGCLSVPGVYEEV
                     RRPVRVGVKAQNENGETFTLDCDGMLAVCVQHEMDHLAGKVFVEYLSALKQDRIRTKL
                     KKRKAREAA"
     gene            5410..>5848
                     /locus_tag="AC16HB_11815"
     CDS             5410..>5848
                     /locus_tag="AC16HB_11815"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5607521.1"
                     /translation="MRIVFAGTPEFAAAALAAIVEAAPANGWSVPLVLTQPDRPAGRG
                     MKLAPSAVRQFARTRSIEVATPPTLSLKKGGNDAASAHAQLRAVAADVLVVAAYGLIL
                     PQAVLDTPRGLPDAGAGRVRAINIHASLLPRWRGAAPVARAIEA"
BASE COUNT          890 a         1977 c         2004 g          977 t
ORIGIN      
        1 cttcgatggt cgccgcgcgc gtggccggcg tgcccagccc cttgccggcc atcgcggcgc
       61 gcagttcctc gtcgtcgatg aacttgcccg cgccctccat tgccgacagc agcgtggcct
      121 cggtgtatcg cgccggcggc ctggtctgga gcgcagcggc ctcgatcgcc tccgtactca
      181 cgcgctcgtt cggatcgacc ttcaccagcg acgcctgttc ttcctgtgcc tccttgccgt
      241 acaccgcgag ccaaccgggt tcgaccagca ccttgccttc ggtcttgaag ccgtgcgact
      301 ccaccgtcgt gatgcgcgtc gtgaccaggt actcggccga cgggaagaag atggccagaa
      361 agcgcttgac cacgaggtcg tagatcttcc cttccgcgtc ggtcaggtgc ttgggctcct
      421 gcagcgtcgg gatgatcgcg aagtggtccg ataccttggc attgtcgaag atgcgcttgt
      481 tcggtcgcac ccagtcgttc ttctggatcg ccttggcgaa ctgcgcgtaa gcgcggttgc
      541 cgccgatcgc gtcgatcgtc ttcttgacga ccttcacgta gtcctcgggc agcgcgcgcg
      601 agtcggtgcg cggatacgtg agcaccttgt gccgctcgta cagcgcctgc gccagcgcga
      661 gcgtggtctt ggccgagaag ccgaaccgtc cgttggcctc gcgctgcagg gaggtcaggt
      721 cgaacagcag cggcggcgcc tgcgtggagg gctttgtttc ctcgctgacg gtgccgctct
      781 tgcccgcgca ggcggcgacg atcgcttccg cccgcgcctt gtcccatagc ctttcggcac
      841 gaccctcggg gtcgtcgtcc cgcttcttga aatccggatc gaaccagcgg ccttcgtacg
      901 tgcccgccct ggcaaggaag gtcgcgcgca cttcccaata gtcgcgcggc tcgaactgct
      961 ggatcttctc ctcgcgctcg accaccagcg ccagcgtggg cgtctgcacg cggccgaccg
     1021 tggtgaggaa gaagccgccg tccttgctgt tgaacgcggt gagcgcccgc gtgccgttga
     1081 tgccgacgag ccagtcggcc tcggcgcgcg agcgggccgc ctcttccagc cggtgcatct
     1141 cggcgtcgct gcgcagcgct tcgaacgcct cgcggatcgc cgccggcgtc attgactgca
     1201 gccacagccg ctggatcggc tgtttcgcgt gcgcgtactg cacgatgtag cggaagatca
     1261 gctcgccctc gcgcccggcg tcgcaggcgt tgatcagtgc gcgcacatcc ttgcgcttga
     1321 tcagccgcgc gagcagcttc agccggtcct cggccttctt gtcgatcggc gccacctcga
     1381 acgcgggcgg gatcatcggc aggtgcgcga agctccattt accgcgctgc acctcgaccg
     1441 ggttcgacaa ctgcaggagg tggccgacgg cgaacgccag cacgtactcg tcggactcgt
     1501 aatagtcctg gtgcttggtg aagccgccga gcgcgcgcgc aatgtcggcg gcgacggaag
     1561 gcttttcggc gatgatcagc gtcttgctca tgatgcaggc ggatgaaaaa gaaaccgccg
     1621 ttcaaggcgg tttccttttt tcagactaac accgaaaccc ggggcgggcg caagtcagtg
     1681 caacgcccga tcctcgccgt cgtcgagcaa ctcctcgagc agcaggatgc cgatgtcggt
     1741 ctgcaggctc cacagcacca tcagcacgat caccttcagt ttcgcgagcg gcaccgggga
     1801 ctcttcggtc gccagcgcgc gctcgaccac gatctcgcgc tgcgccggcg tcagcgcgcc
     1861 gctggcttcg aggcaggaca ggaagctcat cacgtcggtg ccgaggcgcg cggcctcgaa
     1921 ggcggcgaag acgcgcgtgg cgccggcggc aggctcgcgc aggatctgcg agtcctgcgt
     1981 cacgcgctgc aatccctgca gccaggcgat cgcgtcggtg atctcctcgg cgtcgaaacc
     2041 cgcggcggcg agtttgcggg tcagcgcgtc gccgtcgggg caggcgttga gcgcgccgta
     2101 gttttcgtac aggtaaacga ggacgtcgaa catcggcgcg tcgcactcta tccggttcac
     2161 cgtggcggcg caaatgtcgc cgttgcgttc accgcaacct ctggtagcga ttaccgggca
     2221 gacgcgcaat atcttgggcc agctcgagct cgagcaactc ggcggtcagg ctgcccgcgt
     2281 cgcgcccggt gcgcgcggcg agcgtgtcga ggtcgaccgg gtcgtagccg agcgcgtcca
     2341 gcagcgcact ggcgggcgcg gcttctgccg gcggcggcgc cgcgatcgcc ggcatccgca
     2401 gttcactcag gatgtcggcc gcctcctcga cgagcttggc gccctccttg atcagtcgat
     2461 gacagccctt ggccatcggc gaatggatcg accccggcac cgcgaacacc tcgcgcccgg
     2521 tctcggccgc caggcgcgcg gtgatcagcg agccggagcg cagcgccgct tcgaccacga
     2581 gcacgccgcg tgccagcccg gcgatgatgc ggttgcggcg cgggaagttg tgcgcgatac
     2641 cgggcgtgcc gagcgcgaac tcggagatca ccacgccacg ctcgcggatc gccagcgtca
     2701 acgccttgtt cgacgccgga tagatcacgt cgacgccggt gccgaccacc gcgacagtcg
     2761 aatcggccgc gccctccgcc gcagcctgca gcgcgccgcg atgcgccgcc gcgtcgatgc
     2821 ccagcgccag gccgctggcg atcgtcagcc ccgcctgcgc gagccggtgc gcgaaagcgg
     2881 cggcggtgtc cgtgccttgc cgcgtcgcgt tgcggctgcc gacgatggcc agcgtcggac
     2941 ggttgagcaa ctcgaggcgg ccgatcgcat agagcagggg cggcggatcg ctcgtggcga
     3001 gcagcgcctg cggatactgg gcatcgggga gcgtcagcaa ggcattcgat tcgcttgcat
     3061 cgagccaggc ctcggcggac gcgatcgcgt gctccgtcgc ggggtcggcg ggagcggcga
     3121 gcgtctgtgc cagcgcctgc ggtacgtagc ggcccagtgc ggacgcactg gcgtcgaaga
     3181 tcgccgccgg cagtccgaat gcgccgagca gcgcgcgcgc ggtgcgcggc ccgacgtcgg
     3241 gcgtcagcga caggcgcagc caggcggcgc gctcgatgcg gtccatgcgt gcaaaacaaa
     3301 agcgccggcg gggccggcgc ggtgaggagc gaggttccgg ttcagaacca ggtgaacggg
     3361 ttgtaccagc gtcgctgcga gcttgcttcc ggcgcgcggg cgaccggaac cgctgcgccg
     3421 ttgcccgttg cggtcaagtc ggcctcggtc gaatcgggat cggtgaaccg atcgccgacc
     3481 ttcaccggtt tggtcacccg catcaccagg ccgtaggaga tcgtctcggc cacgcggaac
     3541 acgaacagtt cgccgatgcg ctcgtcgggc agcttgacga actcgcggcc gtcgacggtg
     3601 cggtcgcgga tcgtggcgcc cgtttccagc agcgccatca cgtggccgac ttccagcccg
     3661 tcgcgcgcac cgcgattcag cgcgacgatg ctctgcgtgc cggcctggtt gacgccgctg
     3721 tagatcgaaa tgatgcggcc gttgagcgcg cgctcgggtc gatgcggcac gtagttcacc
     3781 agcggctgca cggtcacggg caccagccgg tcgccgacgc cgatctcttc cttgctctcc
     3841 tccacgcgca gcgtggtgac ttcgccgccc ttggcgacgc gggcgacgcc gagatagaag
     3901 gcctcgtatg cgatcgggtt cttgcggttc tcgtcatccg ggtcgtacag cggacgcgcg
     3961 ggacggaaca cgtggtagtc gcgcaccttg tcgtcggtga cgccgcgcgc gtaggcggtg
     4021 tcgccacggc ccaggaacac gcggccttcc tgcgtcgcga cgatgcgcgc gtacttgttc
     4081 agttcgtcgg cggtgaccac cagcgggcgc gacaggaacg gctcgatcac cttggccggt
     4141 atgctgctga tcgcctgctt gtcggcgccg agatcgcgca cgcgcggcga cacccgcacc
     4201 acgtcacccg gcgcgctgcc ctctcccgca agttcgagcc gcgcgcgatc gccggtcttc
     4261 acgagccgca gcatctgccc cggatagatc aggtgcggat tgcggatctg atccttgttc
     4321 atgccccaca actcgggcca gcgccatggc gtcgtcagga agatgctgga gatgtcccac
     4381 agcgtgtcgc ccgtcttgac cgtatacgta tcgggagcat tcggcgccag tgcggacagc
     4441 ggcacgcccg cctgcgcgac ctgatcggcc acgccgcgct gctgctcggt caccggccag
     4501 cgcacgctcg cgggctcgac cgcgcgcgcc tgttggctca cgcaccacgc ggccgcgacg
     4561 atcagtgcgc cggtcagcgc cgcacgcatg cgcaggcggc ggacgtcgag gcttcgggat
     4621 tgcattttca ttcggtttcc ttttctcgcc ccgcctcggc gcggacagcg gcgccgaggc
     4681 gccgcgctgg atcgcaacaa gatcacaagg cgcttaaaat tctgccccta atgccttgat
     4741 atcgcaacgc taatcggctg aagggatgaa ccggatgtgg cgaagccgat cgattgaggc
     4801 aatactgaaa caacggcgta cgcaagacgg cgcgcgtcgt ccattgatcg gacagacatt
     4861 ttcgtggcca gactgcccat tctcgagtac cccgatccgc ggttgaagaa gatcgcgcgg
     4921 ccggtgcatg tgttcgacgc gcgcttgcgc aaactgattt ccgacatggc cgagacgatg
     4981 tattcggcac ccggaatcgg cctggccgcc acgcaggtcg acgtgcacga gcgtgtgatc
     5041 gtgatcgacg cgtcggagac caaggatcag ctgcgcgtgt tcatcaaccc ggagatcctg
     5101 tgggcgtcgg aagaaacgac gctgtgcgag gaaggttgcc tgtcggtgcc cggggtttac
     5161 gaggaagtgc ggcggccggt gcgggtcggc gtcaaggcgc agaacgaaaa cggcgagacg
     5221 ttcacgctcg actgcgacgg catgctcgcg gtgtgcgtgc agcatgagat ggatcatctc
     5281 gccggcaagg tgtttgtcga gtacctgtcc gcgctgaagc aggaccgcat ccggaccaag
     5341 ctgaagaaac gcaaggcgcg ggaagcggcg tgatcagcgc gcgcgcatag cggaccgcgc
     5401 cggcggatca tgcgcatcgt ctttgcgggc acacccgagt tcgctgcagc ggcgctggcg
     5461 gcgatcgtcg aggcggcacc agcgaacggc tggagcgtgc cgctggtgct gacgcagccg
     5521 gaccgtcccg cgggccgcgg catgaagctc gcaccgagcg cggtccggca attcgcacgc
     5581 acacgctcga tcgaagtcgc cacaccgccc acgctcagcc tgaagaaggg cgggaacgac
     5641 gcggcgtctg cgcatgcgca attgcgtgcc gtcgcggccg acgtgctggt cgtcgccgcc
     5701 tacgggctga tcctgccgca ggccgtgctc gacacgccgc gcggactgcc cgacgccggc
     5761 gctggacgcg tcagggcgat caatatccat gcgtcgctgt tgccgcgctg gcggggcgct
     5821 gcgccggtgg cacgcgcgat cgaggcgg
//
LOCUS       DBNHBY010000476         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1114706, whole genome shotgun sequence.
ACCESSION   DBNHBY010000476 DBNHBY010000000
VERSION     DBNHBY010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1114706"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /gene="acs"
                     /locus_tag="AC16HB_11820"
     CDS             <1..1836
                     /gene="acs"
                     /locus_tag="AC16HB_11820"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR5607522.1"
                     /translation="LAREHVKWHKPFTQVLDESNAPFYKWFADGELNASYNCLDAQVE
                     AGNGDKVAIIFEADDGKVTRVTYRALLAEVCKLANALKHHGVRKGDRVLVYMPMSIQV
                     VIAMQACARIGATHSVVFGGFSAKSLQERIIDAGATEVITADGQFRGGKEIPLKPVVD
                     EAFAMGGCEGVRNVIVYKRTGSAVPMQSPRDKWWDDVVKGQAETCEPTWVNAEHPLFI
                     LYTSGSTGKPKGVQHSTGGFLLWAILTMKWTFDHKPSDIFWCTADVGWVTGHTYITYG
                     PLAVGATEVMFEGIPTYPDAGRFWKMIQDHKVTVFYTAPTAIRSLIKANEGNPATHPG
                     KFNLSSLRILGTVGEPINPEAWMWYYNNVGQQRCPIVDTWWQTETGGHLITPLPGATP
                     LVPGSCTLPLPGIMAAIVDETGHDVPNGQGGLLVIKRPWPAMIRTIWGDPDRYKKSYY
                     PEELGGTLYLAGDGSIRDKETGFFRIVGRVDDVLNVSGHRLGTMEIESALVANPIVAE
                     AAVVGRPDDMTGEAVCAFVVLKQARPTGDTAQKLAKELRDWVGKEIGPIAKPKDIRFG
                     DNLPKTRSGKIMRRLLRAIAKGEAITQDVSTLENPAILEQLKVSQ"
     gene            2175..>2364
                     /locus_tag="AC16HB_11825"
     CDS             2175..>2364
                     /locus_tag="AC16HB_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607523.1"
                     /translation="MALLLVIAQTLILVAGMALFGQLIVGAFNWRARHENFVYQLLGI
                     VTRPVVRAVRVITPRVILD"
BASE COUNT          473 a          714 c          811 g          366 t
ORIGIN      
        1 ctggcgcggg agcacgtgaa gtggcacaag ccgtttaccc aggtgctcga tgagagcaac
       61 gcgccgttct acaagtggtt cgccgacggc gaactgaacg cctcgtacaa ctgcctcgac
      121 gcgcaggtcg aggccggcaa cggcgacaag gtcgcgatca tcttcgaggc cgacgacggc
      181 aaggtcacga gggtcaccta ccgtgccttg ctggccgaag tctgcaagct ggccaacgcg
      241 ctcaaacatc acggcgtcag gaagggtgac cgggtgctcg tgtacatgcc gatgtcgatc
      301 caggttgtca tcgcgatgca ggcatgcgcg cggatcggag cgacgcattc cgtcgtgttc
      361 ggcggcttct cggcgaagag cctgcaggag cgcatcatcg atgcgggggc caccgaggtc
      421 atcacggcgg acgggcagtt ccgaggcggc aaggagatac cgctgaagcc ggtcgtggac
      481 gaggcattcg cgatgggcgg ctgcgagggc gtcaggaacg tcatcgtcta caagcggacc
      541 ggcagtgcag tgccaatgca gtcgccgcgc gacaaatggt gggacgacgt cgtcaagggt
      601 caggccgaga cgtgcgagcc gacgtgggtg aacgcggagc atccgctgtt catcctctac
      661 acgtcgggct cgaccggaaa gccgaagggc gtgcagcact ccacaggggg cttcctgctg
      721 tgggcgatcc tgacgatgaa gtggacgttc gaccacaagc cgagcgacat attctggtgc
      781 accgcggatg tcgggtgggt caccgggcac acgtacatca cgtacggtcc gctcgcggtc
      841 ggtgcgaccg aggtcatgtt cgaggggata ccaacctatc cggacgccgg gcgcttctgg
      901 aagatgatcc aggaccacaa ggtcacggtg ttctacaccg cgccgacggc aatccgctcg
      961 ctgatcaagg ccaacgaagg caatcccgcg acgcatccgg gcaagttcaa cctgtcgagc
     1021 ctgcgcatcc ttggcacggt cggcgagccg atcaatccgg aagcatggat gtggtactac
     1081 aacaatgtcg ggcaacagcg ctgcccgatc gtcgacacat ggtggcagac ggaaaccggt
     1141 ggccatctga tcacgccgct gccgggcgcg acaccgctgg tgccgggttc gtgcacgctg
     1201 ccgctgcccg gaatcatggc ggcaatcgtc gacgagaccg ggcatgacgt tccgaacggg
     1261 cagggcggtt tgctggtgat caagcgtccg tggcccgcga tgattcgcac gatatggggc
     1321 gaccccgacc ggtacaagaa gtcctactac ccggaggaac tcggcggcac gctctatctc
     1381 gcgggcgacg gatcgatccg cgacaaggag acgggcttct tccgcatcgt cggtcgcgtc
     1441 gacgacgttc tgaacgtatc gggacaccgg ctcgggacga tggagatcga atcggcgctg
     1501 gtggccaacc cgatcgtggc tgaagcggcc gtcgtcggcc ggcccgacga catgacgggc
     1561 gaggcggtgt gcgccttcgt cgtgctcaaa caggcgcgtc cgaccggcga cacggcgcag
     1621 aagcttgcca aggaactgcg cgactgggtc ggcaaggaaa tcggaccgat cgccaagccg
     1681 aaggacatcc gcttcggcga caacctgccg aagacgcgca gcggcaagat catgcgccgc
     1741 ctgctgcgcg cgatcgcgaa aggtgaggcg atcacgcagg acgtctcgac gctggaaaac
     1801 ccggccatcc tcgagcaatt gaaagtgtcg caatgatgaa tggccgggtt tcggtggagg
     1861 ctgacttgcg cgcagcggaa gtcaagcgcg cgaaggcgcg cggccgagga caaaaccccg
     1921 ccattctgga gcagctcaag gtgagccagt gactcgcggg gtttcgttgg aggctaacgt
     1981 gagcgcagcg aacgttaagc gcacgtacgt gcgcggccgg agacaaaacc cggccatcct
     2041 cgagcaattg aaggtcagcc agtaagatag aacgaacggc acaacgcggc gcaccgacgc
     2101 cgcgggccgg tcagggacga acgggccgac gcactcatgc gcgagcccgg gagagagaca
     2161 gaaggggaca acgcttggcg ttgcttttgg tcattgctca gacgctgatc ctggtcgccg
     2221 gcatggcgct gttcggccag ctgatcgtcg gcgccttcaa ctggcgcgcg cggcacgaga
     2281 atttcgtcta ccaactgctg ggcatcgtca cccggccggt cgtgcgcgcg gtgcgcgtga
     2341 tcacgccgcg cgtgatcctc gacc
//
LOCUS       DBNHBY010000477         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_436492, whole genome shotgun sequence.
ACCESSION   DBNHBY010000477 DBNHBY010000000
VERSION     DBNHBY010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_436492"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..512)
                     /gene="folK"
                     /locus_tag="AC16HB_11830"
     CDS             complement(15..512)
                     /gene="folK"
                     /locus_tag="AC16HB_11830"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR5607524.1"
                     /translation="MKRRAYIGLGANQGDVMETAQAAIDAIRSIERCDFIAASPFYQS
                     APIESEGPDYINAVVAVDTLHDPYALLLHLLDLEIMLGRKRRIPNSPRNAPRKVDLDL
                     LLMGDLILYSTPLMLPHPRLHQRAFALRPLVDLAPDVEIPGLGTAAKFLRAAADQRVT
                     PWPAR"
     gene            complement(548..>1837)
                     /gene="pcnB"
                     /locus_tag="AC16HB_11835"
     CDS             complement(548..>1837)
                     /gene="pcnB"
                     /locus_tag="AC16HB_11835"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR5607525.1"
                     /translation="PKHYAVAEHRIDRALVPRSAMRVCETLQKAGCDAYIVGGAVRDL
                     LLGIAPKDFDVATNATPEQVKSHFRRAIIIGRRFRLVHVMMGPETIEVSTYRAPVGEE
                     VATDEHGRVLADNVFGQRHEDAARRDFTINAIYYDPTTETVLDYHDGLKDLRRKRLRM
                     IGDAEARYREDPVRMLRAVRFAAKLGFEIDAATRAPIARLATLIENVPAARVFDEMLK
                     LLTSGHSVACILRLRAEGLHHGLLPMLDVILEQPEGERFVMLALSRTDERVRSGKHIA
                     PGFLFATLLWHEVLKRWKEREARGEHRIPALDAAIDDVLDAQTEKLAIQRRYTSDMRE
                     IWMLQPRFERRQGGAPYRLLEHLRLRAGYDFLMLRCAAQEAAAELGEWWTHFMHATAE
                     EREQLLAAGRAAQPGGAPRRRRRRRRGNAAAGAPPPA"
BASE COUNT          255 a          653 c          642 g          287 t
ORIGIN      
        1 gcgcgcggcc gcgcttagcg cgccggccag ggcgtgacgc gctggtccgc ggccgcgcgc
       61 aggaacttgg cggccgtccc gagtcctggg atctcgacgt ccggcgccag atcgaccagc
      121 ggccgcagcg cgaacgcgcg ctggtgcagg cgcgggtggg gcagcatcag gggcgtgctg
      181 tacaggatca ggtcgcccat cagcagcagg tcgagatcga ccttccgcgg cgcattgcgt
      241 ggcgagttcg gtatgcgccg cttgcggccg agcatgattt ccaggtcgag caggtgcagc
      301 agcagtgcat acgggtcgtg cagcgtgtcg accgcgacca cggcattgat gtagtcgggg
      361 ccctcggatt cgatcggcgc ggactggtag aacggcgacg cggcgatgaa atcgcagcgc
      421 tcgatcgagc ggatcgcgtc gatcgcggcc tgcgcggtct ccatcacgtc gccctggttg
      481 gcaccgaggc cgatgtatgc gcgtcgcttc aagtctcccc caccgcccat cgatcgctgc
      541 gcgcctgcta ggccggcggc ggggctcccg ccgccgcgtt gccccgccgg cgtcgccgcc
      601 ggcgtcgcgg cgcgcccccc ggttgcgcag cacgcccggc cgccagcaac tgctcgcgtt
      661 cctcggcggt cgcgtgcatg aagtgcgtcc accactcgcc gagttccgcc gccgcttcct
      721 gcgcggcgca gcgcaacatc aggaaatcat acccggcgcg cagccgcaga tgttcgagca
      781 gacggtacgg cgcgccgccc tggcggcgct cgaaccgcgg ctgcagcatc cagatctcgc
      841 gcatgtcgga cgtgtagcgg cgctggatcg ccagcttctc cgtctgcgcg tcgagcacgt
      901 cgtcgatcgc cgcgtcgagc gccgggatgc gatgctcgcc gcgtgcctct cgctccttcc
      961 agcgcttcag cacctcgtgc cagagcaggg tggcgaacag gaagccaggc gcgatgtgct
     1021 tgcccgagcg cacgcgctcg tcggtgcgcg acagcgccag catcacgaag cgctcgccct
     1081 ctggctgctc caggatcacg tccagcattg gcagcaggcc atggtgcagc ccttcggcgc
     1141 gcagccgcag gatgcacgcg accgagtggc cgctggtcag cagcttcagc atctcgtcga
     1201 acacgcgtgc cgccggcacg ttctcgatca gggtcgccag gcgcgcgatc ggtgcgcgcg
     1261 tcgccgcgtc gatctcgaaa ccgagcttgg ccgcgaagcg caccgcgcgc agcatccgca
     1321 ccgggtcctc gcggtagcgc gcctcggcgt cgccgatcat gcgcaggcgc ttgcgccgca
     1381 ggtccttcag cccgtcgtga tagtcgagca ccgtctcggt ggtcgggtcg taatagatcg
     1441 cgttgatggt gaagtcgcgc cgcgccgcgt cctcgtggcg ctggccgaac acgttgtcgg
     1501 cgagcacgcg gccgtgctcg tcggtggcga cttcctcgcc gaccggcgcg cgataagtgg
     1561 acacctcgat cgtctccggg cccatcatca cgtgcaccag gcggaaacgg cggccgatga
     1621 tgatcgcgcg acggaagtgc gacttgacct gctccggcgt ggcgttggtg gcgacgtcga
     1681 aatccttcgg cgcgatgccc agcagcaggt cgcgcacggc gccgccgacg atgtaggcat
     1741 cgcagcccgc cttctgcagt gtctcgcaca cgcgcatcgc cgaccgcggc accagcgcgc
     1801 ggtcgatccg gtgctcggcg accgcgtagt gcttcgg
//
LOCUS       DBNHBY010000478         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_234377, whole genome shotgun sequence.
ACCESSION   DBNHBY010000478 DBNHBY010000000
VERSION     DBNHBY010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_234377"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1342)
                     /locus_tag="AC16HB_11840"
     CDS             complement(326..1342)
                     /locus_tag="AC16HB_11840"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR5607526.1"
                     /translation="MTTNTTPRPAFGTVLAERMSIARFADGRWSGDELVATAPIPMHP
                     GAHVLHYASSCFEGFKAYRWADGSVRVFRMDRHIERMWQSARLLYLPVPDAAQLERMI
                     LQTIDANRAAVPEPPGALYMRPVLFGTMPNIGAASQPSNEATLIVLASPVWDYFAGGM
                     KPLRIYIEAERHRTAEHLGVVKTGGNYAAAMGPTLAAKEQFKIDQVLFCPGGSVQETG
                     AANFLLIREGHILTRSLDSTFLHGVTRDSLLTMARSQGYKVEERVFDVAEMLEWVKTG
                     EAALSGTAAVLAGVGTLVLKDGREIAVGGTGDVGPVTQKLRAALVAIQNGVMPDTFGW
                     TRKV"
     gene            complement(1408..>2416)
                     /locus_tag="AC16HB_11845"
     CDS             complement(1408..>2416)
                     /locus_tag="AC16HB_11845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5607527.1"
                     /translation="APSATSPAVASETKQEKPSPAAAATSAAGAGEVKSEPGEVTRIK
                     ASPATRSKAREAGIDLRQVAGSGPSGRITPKDFAAALSGTPGRATAEAGTATHGLRAR
                     TGIDEVKVIGVRRIIAQRMTDAARTIPHFSYVEEVDVTDLEALRSHLNSKLPKGAPAL
                     TYLPFLAAALARVLEDFPQCNAHYDAERGVLLRHRAVHLGVATQTPDGLKVPVVRHAE
                     ARTIWDLSDEIRRVSEAARTGRATREELSGSTITITSLGRMGGIVSTPIINAPEMGII
                     GVNKAIERPVVFNGSVAIRRIMNLSSSFDHRFVDGYDAAAMILALKDLLEHPATIFIH
                     G"
BASE COUNT          349 a          822 c          848 g          397 t
ORIGIN      
        1 cagcgaacgt ttcgatgcga acggtggcct tgctggtcgc gacagtcgac ggcgatggcg
       61 cggcacaggc cgcaatcgag caccagccga gcagcgtgaa cacggccagg gtggccgtgc
      121 gcctgtagga cagcgtagcg gtcgtcggcg agtgcatctg cagatccgtg acaggaaggg
      181 cgctgcgtca ttcgcgcacg gcgcccgagt ggagtcagtc ggcgcgggcc gacaggcgcc
      241 gaagcccagg tgttgtggtc ttccagcaga tctgcccgcg ccggtgtcga ccgcgacggg
      301 ccggccactc ccggccgcgt tccggctaga ccttgcgcgt ccagccgaac gtgtccggca
      361 tcactccatt ctggatggca acgagtgccg cgcgcagctt ctgcgtgacc ggcccgacgt
      421 cgcccgtacc gccgaccgcg atctcccggc cgtccttcag cacgagcgtg ccgaccccgg
      481 ccagcaccgc ggccgtgccg gacagcgccg cttcgcctgt cttgacccat tcgagcatct
      541 cggcgacgtc gaacacgcgt tcctcgacct tgtagccctg gctgcgcgcc atggtcagca
      601 gcgagtcgcg cgtcacgccg tgcaggaacg tcgagtcgag gctgcgcgtg aggatgtggc
      661 cttcacgaat cagcaggaaa ttcgccgcgc cggtctcctg caccgatcca cccgggcaga
      721 acagcacctg gtcgatcttg aactgctctt tcgcggcaag cgtcgggccc atggccgcgg
      781 cgtagttgcc gccggtcttg accacgccga gatgctccgc cgtgcgatgt cgttcggcct
      841 cgatgtagat ccgcagcggc ttcatgccgc cggcaaagta atcccacacc gggctcgcca
      901 ggacgatcag cgtggcctcg ttggaaggct gcgatgcggc gccaatgttc ggcatggtgc
      961 cgaacagcac cggccgcatg tacagcgctc cgggcggctc ggggacggcc gcgcgattgg
     1021 cgtcgatggt ctgcaggatc atgcgctcga gctgcgccgc gtccggcacc ggcaggtaca
     1081 gcagccgtgc gctctgccac atgcgctcga tgtggcggtc catgcggaac acgcgcacgc
     1141 tgccgtcggc ccagcggtag gccttgaagc cttcgaagca gctgctggcg tagtgcagca
     1201 cgtgcgcgcc cgggtgcatc ggaatcggtg ccgtggcgac cagctcgtcg ccgctccacc
     1261 ggccatcggc aaagcgcgcg atgctcatgc gctcggccag tacggtgccg aaggcggggc
     1321 gtggggtggt gttcgtagtc atgcggtcgg ttcccgtgcg cggcatggcc gcgtatcgaa
     1381 ttgcaggccg tcgattctgc gcggaattca gccgtggatg aagatcgtgg ccgggtgctc
     1441 cagcaggtcc ttcagcgcca ggatcatcgc ggcggcgtcg tagccgtcga cgaagcggtg
     1501 atcgaacgac gacgacaggt tcatgatgcg acggatggcg accgatccgt tgaacaccac
     1561 gggccgctcg atcgccttgt tgacgccgat gatccccatc tccggggcat tgatgatggg
     1621 cgtcgacacg atgccaccca tgcggcccag gctcgtgatg gtgatggtcg agcccgaaag
     1681 ctcctcgcgg gtcgcgcgac cggtgcgcgc ggcctccgac acccggcgga tctcgtccga
     1741 caaatcccag atcgtgcgcg cctcggcatg acgcaccacg ggcaccttca gtccgtccgg
     1801 agtctgcgtg gcgacgccca ggtgcaccgc acggtggcga agcagcacgc cgcgctcggc
     1861 gtcgtagtgc gcgttgcact gggggaagtc ctccagcacg cgagccaggg ccgcggccag
     1921 gaagggcagg tacgtcaggg cgggcgcgcc cttgggcagc ttcgagttca ggtggctgcg
     1981 cagtgcctcg aggtcggtga cgtccacttc ctcgacgtac gagaagtgcg ggatcgtgcg
     2041 cgcggcgtcg gtcatgcgct gcgcgatgat gcgtcgcacg ccgatcacct tcacttcgtc
     2101 gatgccggtg cgggcccgca gcccgtgcgt cgccgtgccg gcctcggccg tcgcgcgccc
     2161 gggtgtaccg gacagcgccg cagcgaaatc cttgggcgtg atgcggccac tcggcccgga
     2221 gcccgccacc tggcgcaggt cgatgccggc ttcgcgcgcc ttgctgcgcg tggcgggcga
     2281 cgccttgatg cgcgtcacct cgccaggctc gctctttact tctccggcgc ccgcggcaga
     2341 ggtcgctgcg gcggcgggtg acggcttctc ctgcttcgtc tcgctcgcga cggcgggaga
     2401 ggtcgcgctc ggcgcg
//
LOCUS       DBNHBY010000479         1641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1384503, whole genome shotgun sequence.
ACCESSION   DBNHBY010000479 DBNHBY010000000
VERSION     DBNHBY010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1384503"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1641
                     /locus_tag="AC16HB_11850"
     CDS             <1..>1641
                     /locus_tag="AC16HB_11850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5607528.1"
                     /translation="ADDVYAFGTMLHELIAGHPPFYPDITPQRVRDETPPALAGNPPP
                     PGLLRDLVAGCLAKSPAARPASMNEVRERLQYALAEVERIDGATVASGAADARPARPA
                     PAWVPQPPADAAPISATWRRSTNEVRDDRALHREGFRNGLMAAALLFAVAALGFVVFV
                     LPRVVAERTPEHAAAQAPAKPRAGQPTASPAPATESLEQQAELMRGAQERRAPLAAEL
                     QQLEARDVGTWGGAAIAAVRTALAAGDTAFAARDFRGALTQFDAAAKGLESLRQRAAV
                     VVGERLAAARVAFDAGRSQDAQAQYEAALRVDPANAVARTGLERAKKLDGVLREMSAA
                     ATAEQAGDAAAAIAAYERALRLDPATAAARDGIARLQARASNDAYASAVASAQAALAR
                     RDYAAAQAGFERAGRLRPGAPEVVEGLEQVRRAAETKSLSATLQRALDAERGERWAES
                     LALYRDALKTDAALRPAQDGVERVEPRAMLDAELQSFLERPDRLYSPSGRDVARNVLE
                     RARATAGGGPRLKQQVAQLAQLLQQAETPIRVALASDNA"
BASE COUNT          229 a          582 c          611 g          219 t
ORIGIN      
        1 gccgacgacg tctacgcgtt cggcacgatg ctgcacgagc tgatcgcggg acacccgccg
       61 ttctatcccg acatcacgcc gcagcgcgtg cgggacgaga ccccgcccgc gctcgcgggt
      121 aacccgccgc ctccgggtct cctgcgtgac ctggtcgcag gttgcctcgc caagtcgcct
      181 gccgcgcgac ctgcgtcgat gaacgaggtg cgtgaacgac tgcagtacgc actggcggag
      241 gtggaacgga tcgatggcgc gacggtggcg agcggtgcgg ccgacgctcg tccggcgcgg
      301 cccgctccgg catgggtgcc gcagccgccc gccgacgcgg cgccgatcag tgcgacctgg
      361 cgcaggtcga ccaatgaggt gcgcgacgat cgtgcgctgc accgtgaagg atttcgcaac
      421 ggcctcatgg ctgccgcgct gctctttgcc gtcgccgcgc tgggcttcgt cgtgttcgtg
      481 ctgccacgcg tcgtcgcgga gcgcactccc gagcacgcgg cggcccaggc gcccgcgaag
      541 ccccgcgccg ggcagccaac ggcctcgcca gctcccgcga cggaatcgct cgagcagcag
      601 gctgagctca tgcgtggtgc gcaggagcgt cgtgcgccgc tggccgcgga attgcagcag
      661 ctcgaagcgc gtgacgtcgg cacgtggggc ggcgcagcga tcgcggccgt ccgcacggca
      721 ctcgctgcgg gcgacaccgc gtttgcagca cgcgatttcc gcggcgcgct gacgcagttc
      781 gacgcggcgg ccaaaggact cgagtcgctg cgccagcgtg cggctgtcgt cgtgggcgaa
      841 cgactcgccg cggctcgcgt cgcgtttgac gcgggacggt cgcaggacgc acaggcgcaa
      901 tacgaggcgg cgctgcgggt cgatccggcc aatgcggtgg cgcgcacggg gctcgagcgt
      961 gcgaaaaagc tggacggcgt gttgcgcgag atgtcggctg ccgcgacggc cgagcaggcc
     1021 ggggatgccg ctgcagccat cgccgcttac gagcgcgcgt tgcggctgga tccggccacc
     1081 gctgccgcac gcgacggcat cgcacgcctg caggcgcgcg ccagcaacga tgcctacgca
     1141 tccgccgtag catcggcaca ggctgcgctc gcacgccgcg actacgccgc ggcgcaggcg
     1201 ggtttcgagc gtgccggtcg actgcgtccc ggcgcacccg aggtggtcga ggggctggag
     1261 caggttcgcc gcgcagccga gacgaagtcg ctgtctgcca cgctgcagcg cgcgctcgac
     1321 gccgagcggg gcgagcgctg ggcggagagc ctcgcgctct atcgcgacgc gttgaagacg
     1381 gatgccgcac tgcgcccggc gcaggacggc gtcgaacggg tcgagccacg cgcgatgctc
     1441 gatgctgagc tgcaatcctt cctggagcgg cccgaccgcc tctacagtcc ctcgggtcgc
     1501 gatgtggcgc gcaacgtgct cgaacgtgcg cgggcgaccg ccgggggcgg gccgcggctg
     1561 aagcagcagg tggcccagct cgcgcaactg ctgcaacagg ccgagacgcc gatccgcgtc
     1621 gcgctcgcct ccgacaatgc g
//
LOCUS       DBNHBY010000480         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1723113, whole genome shotgun sequence.
ACCESSION   DBNHBY010000480 DBNHBY010000000
VERSION     DBNHBY010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1723113"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="AC16HB_11855"
     CDS             <1..765
                     /locus_tag="AC16HB_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607529.1"
                     /translation="SAAWAQDKFIVVASTTSTEQSGLFSHLLPAFKQASGIDVRVVAV
                     GTGQALDMGRRGDADVVFVHDTAAEEKFVAEGYAVKRLPVMYNDFVLVGPKADPVGVH
                     GNDIAAALRKLASSDVPFISRGDRSGTHAAELRLWKLVGVDVAAKMPIGYKACGCGMG
                     PALNIGASSNAYVLTDRGTWLSFKNRGELVILVEGDKTLFNQYGAMVVNPAKFPHVKQ
                     ALAQAFVDWIVSPAGQSTIASYRINGEQLFFPNAGR"
     gene            851..1990
                     /locus_tag="AC16HB_11860"
     CDS             851..1990
                     /locus_tag="AC16HB_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5607530.1"
                     /translation="MRSRGVHLQYAFQPGGQHGADVENPLFDLLSALHEHGSIHHAAR
                     AMGASYRHVWGALKHWEQVLGESLVTWTQGQPARLTPFAQRLLWAETRARTQLTPHIE
                     ALRAELERVLAEALDGTQQVLTVFASHDLALPLLRERASVAHRLHIELKFAGSMDALR
                     ALADGRCHVAGFHVPQLVGGGHVFARELKPLLKPGRHKLIGCTRRTQGLMVAASNPLQ
                     LHDLAGLAQGGIRFINRQRGSGTRLLTEHLLARFGIAAERIAGFTEAAEESHIAIAAA
                     IASGAADAGVGIEAAARQFGLDFVPLVQEDYFLVCLRDALDQPAVAKLRDVLRDPIWQ
                     RSVAALPGYDVVHGGEILALTRALPWWRFRKQKSERPMRPNRAAE"
BASE COUNT          390 a          793 c          828 g          392 t
ORIGIN      
        1 tcggccgcgt gggcacagga caagttcatc gtggtggcat cgaccacctc gacggagcag
       61 tcgggattgt tctcgcacct gctgcctgcg ttcaagcagg cgtcgggcat cgacgtgcgg
      121 gtggtcgcgg tcggtaccgg ccaggcactc gacatgggcc gccgcggcga cgccgacgtg
      181 gtgttcgttc acgacacggc ggccgaggag aaattcgtcg ccgaaggcta tgccgtcaag
      241 cgcctgccgg tcatgtacaa cgacttcgtg ctggtcgggc cgaaggccga tccggtcggt
      301 gtgcacggaa acgacatcgc cgccgcgttg agaaagctcg cgtcgagcga cgtgccattc
      361 atctcccgcg gcgatcgcag cggaacgcat gcagccgagc tgcgcctgtg gaagctggtg
      421 ggcgtcgatg tcgccgcgaa aatgccgatc ggatacaagg catgcggctg cggaatgggg
      481 ccggcgctca acatcggtgc gtcgtccaac gcctacgtgc tgacggaccg cgggacctgg
      541 ctgtcgttca agaatcgcgg cgagctcgtg atcctagtcg aaggcgacaa gaccctgttc
      601 aaccagtacg gcgcgatggt ggtgaatccg gcgaagttcc cgcacgtcaa gcaagcgctg
      661 gcgcaggctt tcgtcgactg gatcgtatcg ccggccggcc aatccacgat cgcgagctac
      721 cggatcaacg gcgaacagct gttctttccg aatgccggcc gctgaaccga gccggcattt
      781 cgccggtgcg cggagcgccg cgacaggcac cgtgtcctga ccggcgggcc atatcgacta
      841 cgcttgcggg atgcgatctc gcggcgttca cctgcagtac gcctttcagc ccggtggaca
      901 gcacggcgcc gacgtcgaga accccctgtt cgatctgctc tcggcgctgc atgagcacgg
      961 ctcgattcat cacgccgcgc gggccatggg cgcctcgtat cgccatgtgt ggggcgcgtt
     1021 gaagcactgg gaacaggtgc tcggggagtc gttggtgacc tggacgcagg gccagccggc
     1081 gcgcttgact ccgtttgcgc aacggctgct gtgggccgaa acgcgcgcgc gcacgcagtt
     1141 gacgccgcac atcgaggcgt tgcgcgccga gctcgagcgc gtgctggccg aggcgctgga
     1201 cggtacgcag caggtgctga ccgtgtttgc cagccacgac ctggcgctcc cgctgctgcg
     1261 cgagcgtgcc agcgtcgccc accggctgca catcgaactg aaattcgccg gcagcatgga
     1321 cgcgctgcgt gcgcttgccg acggccgctg ccacgtggcg gggtttcacg taccgcagct
     1381 ggtcggaggc ggccacgtct tcgcgcgcga gctcaagcca ttgctcaagc cgggccgcca
     1441 caaactgatc ggctgcacgc gccgcacgca gggcctgatg gtggcggcga gcaatccgct
     1501 gcaactgcac gatctcgccg ggcttgcgca aggcgggatt cgcttcatca atcggcagcg
     1561 cggctccggc acgcggctgc tgactgagca tctgctcgcg cgattcggta tcgctgccga
     1621 gcgcatcgcc ggcttcacgg aggccgccga ggaaagccac attgcgattg cggccgccat
     1681 tgccagcggc gccgccgacg ccggcgtggg gatcgaggcg gccgcacggc agttcggact
     1741 cgacttcgtg ccgctggtgc aggaggacta cttcctcgtc tgcctgcgcg atgcgctcga
     1801 ccagcctgcc gtcgcgaaac tgcgtgacgt cctgcgcgat ccgatctggc aacgcagtgt
     1861 cgcggcgctg cccggatacg acgtcgtgca cggcggcgaa atccttgcgc tgacgcgcgc
     1921 gctgccgtgg tggcgattcc ggaagcaaaa gtccgaacgc ccgatgcggc cgaaccgggc
     1981 ggcagagtaa tgtaccggcc aaccgtgaag ctccgctcga tcgactgtcg aacccgcgga
     2041 tcggtggcgt gccggccagc cggtctgccg ctccccgcct gacctcagct ggaaagtgcc
     2101 ggacgtgtcc gatcatcctg tcttggtccc gtcgagccgc gatcgcgcgg aagcgctcga
     2161 tcgcgatcga accgacggcg ccgtgcaagc cggagggacg cattcgagga tccgtcgctg
     2221 cgcgaccggc ggctccgcag aaaaacgctt cgcgaaccgg cgtctgtcga cgggagacgg
     2281 ttagaagcga tgagcgactt gactctgccc ggtgcgggcg aggcaagcgg atccggacca
     2341 gttcccgcca cctcgcgcgc gcagattccc tcggtcgatc gactgctgag tcgcaaggcc
     2401 ctg
//
LOCUS       DBNHBY010000481         6645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_234766, whole genome shotgun sequence.
ACCESSION   DBNHBY010000481 DBNHBY010000000
VERSION     DBNHBY010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_234766"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="AC16HB_11865"
     CDS             complement(<1..685)
                     /locus_tag="AC16HB_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5607531.1"
                     /translation="MTLKIGIDVGGTFTDFVVVRDGAPPVIYKTLSTPADPSIAVVDG
                     LAEIAASQKPPLSLSAFAATIDTLVHGTTVTTNATLTSTGAKCGLLTTEGVRDALEMR
                     RGIREEQYNNRYTNVKPLVARFLRAGIRGRLDRNGRELQPLDLDGVREAIDRFRREGV
                     QAVAICFMNSFANPAHEQAAAELVRRELPGAYLSVSTDVLPSIRFYERVSTTALNAYV
                     GPKLNHYLDQ"
     gene            complement(706..2226)
                     /locus_tag="AC16HB_11870"
     CDS             complement(706..2226)
                     /locus_tag="AC16HB_11870"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0032787 - monocarboxylic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR5607532.1"
                     /translation="MDLSDWITGHAQRTPHKAALRFDGRDLSYAAFAQHIGRLASALA
                     ACGVSQGRCVSYLGLNRPEELALLFACARLGALFMPLNWRLAAPEHRERLLDCPPTVL
                     VVEDGFVAQTATYADALPPMTRVAIGDAPAGWHTFDALLARPGGTVGDVQSSPQLPLL
                     VCHTSGSTGQPKGVLLTQQALITNAANSVALHDMTADDRILTNLPLFHVGGLNNQTTP
                     ALQAGATVVLHSKFDPNATFDAIERERITLTVLVPTQLTMMMALLRWHSADFSSLRMI
                     TTGSTIVPEHLIRAVHARGVPLVQIYGLTETCPIAAYLRPEDAQRKAGSTGKAAPHCQ
                     VRVVDGDDRDVAQGARGEILIRGDNVMAGYWKQPQTTAAALAGGWFHSGDIGHFDADG
                     FLWVDGRAKDMIISGGENIAPAEIENLLLECPDVAEVAVVGRPDTNWGEVVVAVVAPK
                     EGRALTSDRVLALLDGRIARYKHPRQVLVVGELPKTALGKVRKEDVRKLVVASPKA"
     gene            complement(2235..3440)
                     /locus_tag="AC16HB_11875"
     CDS             complement(2235..3440)
                     /locus_tag="AC16HB_11875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5607533.1"
                     /translation="MNYKAEIPYGAYWSTPFARWQGSFAHLHAIEFAAHVARNELAKR
                     KIDPRQFDYGVLGFSVPQKHSFYGLPWLTGLVGAKQAGGPTLMQACATGVRTLLAAAQ
                     EVEVGMANTALVVNCDRVSNGPHLYYPNPRGPGGTGAAEDWVMDNFGCDPLGPHSMLQ
                     TAENVAAKHGITTAQQHEVVLLREQQYRRALENDSAFLKRFVTLPFEVPAPGYRKAAG
                     SLAGDEGVSQSTPEGLAKLKPVMEGGTVTFGGQTHPADGNAGLVVCTRDRAREMATDR
                     RIAVRLHGFGLARAPLALMPESTVPAAQRALAQAGRDVQQMDAIKTHNPFAVNDLFFS
                     KQTGVGLASMNNYGCSLVWGHPQAPMGTRAVIELIEELALRGGGWGLFTGCAAGDSAM
                     AVVLEVGDA"
     gene            complement(3464..4138)
                     /locus_tag="AC16HB_11880"
     CDS             complement(3464..4138)
                     /locus_tag="AC16HB_11880"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5607534.1"
                     /translation="MNLALKPVERPLALGEQVYHALRTLLRSGQIPHGQPLQEVQLAE
                     RLGVSRTPVRQAMTRLASEGLLASDGRSFVVPKLTLRDVDDIYEVRFLIEPAAIRSVA
                     AFTTDKAVRAPLDAALAAAAAAHKTGDTEAFSEAHQRFRAAWLALVPNRRLVRAVEQY
                     ADHLQHIRALTLGEPQVRAIVLKGYRRVAAALAVGDGKAAAEAMNATLTEAKRAFSDA
                     MGLAES"
     gene            4311..5282
                     /gene="dctP"
                     /locus_tag="AC16HB_11885"
     CDS             4311..5282
                     /gene="dctP"
                     /locus_tag="AC16HB_11885"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR5607535.1"
                     /translation="MMRTIRIAAAAATALLLAAPAAAQEVTLRAIAFIPKTHPLMAQT
                     QVWVNEVNNALKGKLRINYVGGPEVIPGLQQADAVGKGVIDIAFNPTAYYQSTFPEGG
                     ALILSRKSTSEERAAGGLYDFMVKRHERINLRYIGRALWSPFYLWTSTKVANIDGLKG
                     QKMRTSALYDRFMQALGIVPVTIPEGETYTALEGGTVTGFGWPMAGPRERGWIKSAKY
                     LIDLPFYASNNIVIIMNLDKWKSLPEDVRKEIERVTAAFEPKMVEHYQKAEAEEWALL
                     EKAGVQKIKFSDAENKRYIDTAYKVEWDNLATKVPDIVGELRTLTGN"
     gene            5337..5831
                     /locus_tag="AC16HB_11890"
     CDS             5337..5831
                     /locus_tag="AC16HB_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5607536.1"
                     /translation="MAVLDRWLTRVENVLAALGIAVLIFIIGAVCTEIVLRTFFARPQ
                     VWVIEFSEYGLLYVTFLGTAWLMRQDGHVRVDLLTNALDRDWKRRLALASEAIGCTVS
                     VVLTVFGVIVTLDAHRRGLFKPTVLEFPTWIVLLVIPVGAALLALRFVQRFIALWRRT
                     LEPR"
     gene            5834..>6645
                     /locus_tag="AC16HB_11895"
     CDS             5834..>6645
                     /locus_tag="AC16HB_11895"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5607537.1"
                     /translation="MDWATTLAIIFGALLALMAIGVPIAFAFLAVNVVGAYFFWNGAA
                     GLGQLVMSVYDSVTTFALLPVPLFVLMGELMFRSGIAGRLMDVLDSWIGRLPGRLSVM
                     AVGGGTLFATLTGSAMAGAAMLASLLVPEMEKRGYHRSMSLGPILGSGGLAILIPPSA
                     LAVLLAAIANISVGDFLVVIIVPGILTAIAFVAYIVVRCRITPALAPAYDLPRVSLAL
                     RLRNTVLYGLPLVVVVFLVVGLMFLGVATATESAAAGAFGCFLLSWAYGGLNR"
BASE COUNT          978 a         2227 c         2304 g         1136 t
ORIGIN      
        1 gctggtcgag gtagtgattc agcttcggcc cgacgtaggc gttcagcgcg gtcgtgctga
       61 cgcgctcgta gaagcggatc gacggcagca cgtcggtcga caccgacagg tacgcgcccg
      121 gcagctcgcg ccgcacgagt tcggccgcgg cctgctcgtg cgcggggttg gcgaacgagt
      181 tcatgaagca gatcgcgacc gcctgcacgc cttcgcgcct gaagcggtcg atcgcctcgc
      241 gcacgccgtc gagatcgagc ggctgcagtt cgcgcccgtt gcggtcgagc cggccgcgga
      301 tgccggcgcg caggaaccgc gctaccagcg gcttcacgtt ggtgtagcgg ttgttgtact
      361 gctcctcgcg gatgccacgc cgcatctcga gcgcgtcgcg cacgccttcg gtggtcagca
      421 acccgcactt ggcgccggtg ctggtcagtg tcgcgttggt cgtcaccgtc gtgccgtgca
      481 cgagcgtgtc gatcgtcgcc gcgaacgcgg acaacgacag cggcggcttc tggctggcgg
      541 cgatctcggc cagcccatcg acgaccgcga tcgacggatc cgcgggcgtg gacagcgtct
      601 tgtagatcac cggcggcgcg ccgtcgcgca cgacgacgaa gtccgtgaac gtgccgccga
      661 cgtcgattcc gatcttcagt gtcatcgcct gcgctccctg tctgttcagg ccttcggact
      721 ggccaccacc agcttccgca cgtcttcctt gcgcaccttg ccgagcgccg tcttcggcag
      781 ttcgccgacg accagcacct ggcgcggatg cttgtagcgc gcgatgcggc cgtcgagcag
      841 cgccagcacg cgatcggaag tgagcgcacg gccttccttc ggcgcgacga cggcgaccac
      901 cacctcgccc cagttcgtgt cgggccggcc gaccacggcg acctcggcaa cgtcggggca
      961 ttcgagcagc aggttctcga tctcggcagg cgcgatgttc tcgccgccgg agatgatcat
     1021 gtccttggcg cggccgtcga cccacaggaa accgtcggca tcgaagtggc cgatgtcgcc
     1081 gctgtggaac cacccgcccg ccagcgccgc ggccgtggtt tgcggctgct tccagtagcc
     1141 ggccatcacg ttgtcgccgc ggatcaggat ctcgccgcgc gcgccttgcg cgacatcgcg
     1201 gtcgtcgccg tcgaccacgc gcacctggca atgcggcgcc gccttgcccg tcgatccggc
     1261 cttgcgctgc gcgtcctcgg gccgcaggta ggcggcgatc gggcaggtct ccgtcaggcc
     1321 gtagatctgc accagcggca cgccgcgcgc gtgcaccgcc cggatcaggt gctcgggcac
     1381 gatcgtcgag ccggtcgtga tcatgcgcag gctggagaag tcggcggagt gccagcgcag
     1441 cagcgccatc atcatcgtca gttgcgtcgg caccagcacg gtgagcgtga tgcgctcgcg
     1501 ctcgatcgcg tcgaaggtcg cattcggatc gaacttcgaa tgcagcacca cggtcgcgcc
     1561 cgcctgcagt gccggcgtgg tctggttgtt gagccccccg acgtggaaca gcggcaggtt
     1621 ggtcaggata cggtcatcgg ccgtcatgtc gtgcagtgcg acgctgttgg ccgcattcgt
     1681 gatcagcgcc tgctgtgtca gcagcacgcc cttgggctgt ccggtcgatc ccgacgtgtg
     1741 gcagacgagc agcggcaatt gcggcgagga ctgcacatcg cctacggtcc cgcctgggcg
     1801 cgcgagcagc gcgtcgaacg tgtgccagcc tgcgggtgca tcgccgatcg cgactcgggt
     1861 catcggcggc aaggcgtccg cgtatgtcgc ggtctgcgcg acgaagccat cctcgaccac
     1921 gagcacggtc ggcgggcaat cgagcaggcg ttcgcgatgc tcgggcgcgg cgaggcgcca
     1981 gttcaacggc atgaagagtg ccccgagccg cgcgcacgcg aagagcagcg cgagttcctc
     2041 cggccggttc aaccccaggt acgaaacgca gcgaccctgg cttacgccgc acgcggcgag
     2101 cgcggaggcg aggcgcccga tgtgctgggc gaatgccgca tagctgagat cccggccgtc
     2161 gaagcggagc gccgccttgt gcggcgtccg ctgcgcgtgg ccggtgatcc agtcggacag
     2221 gtccatctgc gcgcctacgc gtcgcccacc tcgagaacga ccgccatcgc agagtcgccg
     2281 gcggcgcagc cggtgaagag tccccagccg ccgccgcgca gcgcgagttc ctcgatcaac
     2341 tcgatgaccg cccgcgtgcc catcggcgcc tgcggatgac cccacaccag cgagcagccg
     2401 tagttgttca tcgacgcgag gccgacgccg gtctgtttgg agaaaaagag gtcgttgacc
     2461 gcgaacgggt tgtgcgtctt gatcgcgtcc atctgctgca cgtcgcgccc ggcctgcgcg
     2521 agcgcccgct gcgcggccgg cacggtcgac tccggcatga gggcgagtgg cgcgcgcgcg
     2581 agcccgaacc catggagccg caccgcgatc cggcgatcgg ttgccatttc ccgcgcgcgg
     2641 tcgcgcgtgc agacgacgag ccccgcgttg ccgtcggccg gatgcgtctg tccgccaaag
     2701 gtgacggtgc cgccttccat caccggtttg agcttcgcca gtccctccgg cgtcgactgc
     2761 gacacgcctt cgtcgcccgc gaggctgccg gccgccttcc tgtatcccgg tgccggcacc
     2821 tcgaacggca gcgtgacgaa gcgcttcagg aacgcgctgt cgttctcgag cgcccgccgg
     2881 tactgctgct cgcgcagcag caccacctcg tgctgctgtg ccgtcgtgat gccgtgcttc
     2941 gcggcgacgt tctcggccgt ctgcagcatc gagtgcgggc cgagcggatc gcagccgaag
     3001 ttgtccatca cccagtcctc ggccgcgccg gtgccgcccg ggccgcgcgg gttggggtag
     3061 tagaggtgcg ggccgttgga gacgcggtca cagttgacga ccagggcggt gttcgccatg
     3121 ccgacctcga cctcctgtgc ggcggcgagc agcgtgcgca cgccggtggc gcacgcctgc
     3181 atcaacgtcg gtccgccggc ctgcttggcg ccgaccaacc cggtgagcca cggcaggccg
     3241 taaaaggaat gtttctgcgg cacggagaat ccgagcacgc cgtaatcgaa ctggcgcgga
     3301 tcgatcttgc gcttcgccaa ctcgttgcgc gccacgtggg cggcgaattc gatggcgtgc
     3361 aggtgggcga agctgccctg ccagcgcgcg aacggcgtgc tccagtaggc gccgtaggga
     3421 atttcggcct tgtagttcat tcgcttctcc tcatgcgccg cgttcagctt tccgcgagtc
     3481 ccatcgcgtc gctgaacgcg cgcttggctt cggtcagagt ggcgttcatc gcctccgccg
     3541 cggccttgcc atcgcccacc gcgagcgcgg cggcgacgcg gcggtagccc ttcagcacga
     3601 tcgcgcgcac ctgcggctcg ccgagcgtca gcgcgcggat gtgctgcagg tgatcggcgt
     3661 actgctcgac cgcccgcacg agccggcggt tcggcaccag cgccagccag gccgcacgaa
     3721 accgctggtg cgcctcgctg aaggcttccg tgtctcccgt cttgtgcgcg gcggcggccg
     3781 cggccagcgc cgcgtcgagc ggtgcgcgta ccgccttgtc ggtagtgaag gccgccacgc
     3841 tgcggatcgc ggccggctcg atcaggaagc gcacctcgta gatatcgtcg acgtcgcgca
     3901 acgtgagctt cggcaccacg aagctgcgac cgtcggatgc cagcagacct tcgctcgcca
     3961 gccgtgtcat cgcctgccgc acgggagtgc gcgacacgcc cagccgctcg gccagttgca
     4021 cctcctgcag cggttgtccg tgcgggatct gtccgctcct caacagcgtg cgcagcgcgt
     4081 ggtagacctg ctcgccgagg gcgagcgggc gttcgacggg tttcagtgcg aggttcaagg
     4141 ggtgtaccgg cgcaagtccg aaactgcgtc gacaggatgc aggacactgt atacacagaa
     4201 aatgacatga atcaaacccg tgactgcgcg aggagtggca agcttgcgcg gcggaaacgg
     4261 cggttcggtt gcagtccggg gcgaaacagc agaggcagga ggagacgacg atgatgcgga
     4321 cgatcaggat tgcggcggca gccgcgacgg cgttgctgct ggcagcgccc gcggcggcgc
     4381 aggaagtgac gctgcgcgcg atcgcgttca ttcccaagac gcacccgctg atggcgcaga
     4441 cccaggtctg ggtcaacgag gtcaacaacg cgctgaaggg caagctcagg atcaactatg
     4501 tcggcgggcc ggaggtgatc ccgggcctgc agcaggccga cgcggtcggc aagggcgtga
     4561 tcgatatcgc gttcaacccg accgcctatt accagagcac gtttcccgag ggcggtgcgc
     4621 tgatcctgtc gcgcaagtcc acgagcgagg agcgcgcggc cggcgggctg tacgacttca
     4681 tggtcaagcg gcacgagcgg atcaacctgc gctacatcgg tcgcgcgctg tggtcgccgt
     4741 tctatctgtg gacgtcgacc aaggtcgcga acatcgacgg cctgaagggg cagaagatgc
     4801 gcacgtcggc gctgtacgac cgcttcatgc aggcgctcgg catcgtgccg gtgacgattc
     4861 ccgaaggcga aacctatacc gcgctcgagg gcggcacggt gaccggcttc ggctggccaa
     4921 tggcgggccc gcgcgaacgc ggctggatca agtcggcgaa atacctgatc gatctgccgt
     4981 tctatgccag caacaacatc gtgatcatca tgaacctcga caagtggaag tcactgcccg
     5041 aggatgtgcg caaggagatc gagcgcgtga ccgcggcgtt cgagccgaag atggtcgagc
     5101 attaccagaa ggccgaggcc gaggagtggg cgctgctcga gaaggccggc gtgcagaaga
     5161 tcaagttctc cgacgccgag aacaagcgct acatcgacac cgcctacaaa gtcgagtggg
     5221 acaacctcgc gaccaaggtg ccggacatcg tcggagagct gcggacgctg accggcaatt
     5281 gaacgtcggg ccgtcgtccg cccggccgcc cgcagcatcg ctgccttgat ttgccgatgg
     5341 ccgtgctcga ccgctggctg acccgcgtcg agaacgtgct ggccgctctc ggcatcgcgg
     5401 tgctgatctt catcatcggc gccgtctgca cggagatcgt gctgcgcacg ttcttcgcgc
     5461 gcccgcaggt gtgggtgatc gagttcagcg aatacgggct gctgtacgtc acgtttctcg
     5521 gcacggcgtg gctgatgcgg caggacggcc acgtgcgcgt cgacctgctg accaatgcgc
     5581 tcgatcgcga ctggaagcgc aggctcgcgc tggcgagcga agcgatcggc tgcacggtgt
     5641 cggtcgtgct gaccgtgttc ggcgtgatcg tcacgctcga cgcgcaccgg cgcggacttt
     5701 tcaagccgac ggtcctggag tttccgacct ggatcgtgct gctggtgatc ccggtcggag
     5761 cggcgctgct cgcgctgcgg tttgtgcagc gattcatcgc gctgtggcgg cgcacgctcg
     5821 agccccggta acggtggact gggcgaccac gctcgcgatc atcttcggcg cgctgctggc
     5881 gctgatggcg atcggcgtcc cgatcgcttt cgcctttctc gcggtcaacg tcgtcggtgc
     5941 ctatttcttc tggaacggtg ctgccggtct cgggcagctc gtgatgtcgg tctacgactc
     6001 ggtcaccacg ttcgcgctgc tgccggtgcc gctgttcgtg ctgatgggcg agctgatgtt
     6061 ccgttccggc atcgcggggc ggctgatgga cgtgctcgac tcgtggatcg gccgcctgcc
     6121 cgggcgactg agcgtgatgg cggtgggcgg cggcaccctg ttcgccacgt tgacgggctc
     6181 cgcgatggcg ggcgccgcga tgctggcgtc gctgctcgta cccgaaatgg agaagcgcgg
     6241 ctatcaccgc tcgatgtcgc tcggaccgat cctcgggtcc ggcggactcg cgatcctgat
     6301 tccgccgtct gcgctcgcgg tgctgctggc ggcgatcgcc aacatctcgg tcggcgattt
     6361 cctggtcgtg atcatcgtgc cgggcatcct gaccgcgatc gccttcgtcg cctacatcgt
     6421 ggtccgctgc cggatcacgc cggcgcttgc gcccgcgtac gacctgccac gggtgtcgct
     6481 ggcgctgcgg ctgcgcaaca cggtgctgta cggcctgccg ctggtcgtcg tcgtgttcct
     6541 cgtcgtcggt ctgatgttcc tcggtgtggc gaccgccacc gagtcggccg ccgcgggggc
     6601 gttcggctgc ttcctgctgt cgtgggccta cggcggattg aatcg
//
LOCUS       DBNHBY010000482         3751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1789657, whole genome shotgun sequence.
ACCESSION   DBNHBY010000482 DBNHBY010000000
VERSION     DBNHBY010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1789657"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC16HB_11900"
     CDS             <1..594
                     /locus_tag="AC16HB_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR5607538.1"
                     /translation="YPDYLLHKMVRVMRGGEEVKMSKRAGEYVTLRDLIDWVGRDATR
                     FFLVSRKADTEFVFDLDLALSQSEENPVYYVQYAHARICSVLAQAAGAGVAIEEDRAL
                     HANLAPLTGAREQALLARLSDYPDALVEATRELAPHQIAFYLKDLAAEFHAFYNAERV
                     LVDDAALRDARLALLLATRQVVRNALAVIGVSAPERM"
     gene            615..1175
                     /locus_tag="AC16HB_11905"
     CDS             615..1175
                     /locus_tag="AC16HB_11905"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5607539.1"
                     /translation="MNSIQRQRGGTVLGFVIGLIVGLAVAVAVAVYITNAPLPFVTKV
                     QRPATHVAPAPGAPLPDPNKPLYGQGKNEPPEETKSAAAPPAVAPAAEKPAEKAPGKE
                     AAPAAEEGTRFLLQAGAFKSPDEADAMRARLALLGLDARIFPIDQGGKTLYRVRLGPY
                     GQLDEINRTRKLLAENSIEAQVVRLK"
     gene            1246..1905
                     /locus_tag="AC16HB_11910"
     CDS             1246..1905
                     /locus_tag="AC16HB_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR5607540.1"
                     /translation="MHLILGRREFLAAAVALPTIALAQAGKKPDAGIDYNELRPAQPT
                     EAPGKIEVLEFFWYGCPHCAAFDPELAAWRKRLSADVAYRRNPVAFSDAQTPHTKIFY
                     TLEALGKVDALHSRVFAEIVGSRKPMLKTDDIADFMAANGIDRKQWLDTFNSFTVATR
                     ASRAAQVWRAYKVDGTPTIGVDGRYTTSPAQANGRAECLRVVDFLIDRIRAEKGGVAK
                     K"
     gene            1996..2769
                     /locus_tag="AC16HB_11915"
     CDS             1996..2769
                     /locus_tag="AC16HB_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607541.1"
                     /translation="MSFNVFITGASSGIGAALASEFARRGAAVGLVARRKAALDELIG
                     RLPGRHFAYAADVTDKDALIAAAHAFERDSGGVDIVVANAGISVGVLTEHYEDLAAFK
                     QVFDTNVLAMAHTFHPFIATMRERRRGTLVGIASVAGIRGLPGSEAYCASKAAAISYC
                     ESLRVELKRFGVRVVTLAPGFIRTPLTARNPYRMPFLMEPDAFARRAVDAIIAGVSYR
                     VIPWQMGLAAKLLRALPNWAFDKLMSNRPYKPRKTEPRP"
     gene            2766..3572
                     /locus_tag="AC16HB_11920"
     CDS             2766..3572
                     /locus_tag="AC16HB_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR5607542.1"
                     /translation="MTWWDAVGQAHAAAAPGFRIVSLVPSLTELLLDLDLREHVVGRT
                     GFYIHPRELVRAIPKVGGTKDVKLERVRELEPTHAIVNIDENELPTVQALREFVPHVI
                     VSHPQAPEDNPALYRLLGGVFGRAPEAERLCADLQRELDACRAIDWARERVLYLIWKD
                     PWMTIAADTYIARTLARVGWDVVRGPGGERGAARYPVIDDLDVEVGRVDRVLLSTEPF
                     MFRAPHVAALRARFAGKCVELIDGEMTSWYGSRAIEGLRFLRRHRAARAP"
BASE COUNT          621 a         1277 c         1278 g          575 t
ORIGIN      
        1 taccccgact atctgctgca caagatggtg cgcgtgatgc ggggcggcga ggaagtcaag
       61 atgtccaagc gcgccggcga atacgtcacg ctgcgcgacc tgatcgactg ggtcgggcgc
      121 gatgcgacgc gtttcttcct ggtctcgcgc aaggccgaca ccgagttcgt gttcgacctc
      181 gatctggcgc tgtcgcaaag cgaggagaac cccgtgtact acgtgcagta tgcgcacgcg
      241 cgcatctgca gcgtgctggc gcaggccgcg ggcgccgggg tcgcgatcga agaggaccgc
      301 gcgctgcacg caaacctggc gccgctcacc ggcgcacgcg agcaggcgct gctggcgcgg
      361 ctgtcggact acccggacgc gctcgtcgag gccacgcgcg aactggcgcc gcaccagatc
      421 gcgttctatc tgaaggacct cgcggccgag tttcacgcct tctacaatgc cgagcgcgtg
      481 ctcgtcgacg acgccgcgtt gcgcgacgcg cggctggcgc tgttgctcgc cacgcgccag
      541 gtggtgcgca acgcgctcgc ggtgatcgga gtgtcagcgc cggagcggat gtgaattcgg
      601 gaacgggatc agcagtgaat tcgattcagc ggcagcgcgg cgggacggtg ctgggattcg
      661 tcatcggcct gatcgtcggg ctggcggtcg cggtcgcggt cgcggtctac atcaccaatg
      721 caccgctgcc gttcgtgacc aaggtgcagc ggccggccac acacgtggcg ccggcgccgg
      781 gcgcgccgct gcccgatccg aacaagccgc tgtacggcca gggcaagaac gagcccccgg
      841 aagaaacgaa gtcggcggcg gcgccacccg ctgtagcgcc cgctgcggag aagccggccg
      901 agaaggcccc cggcaaggag gcggcaccgg cggccgagga aggcacgcgc ttcctgctgc
      961 aggctggggc cttcaagtcg cccgacgagg ccgacgcgat gcgcgcacgg ctcgcgctgc
     1021 tcggactcga tgcgcggatc ttcccgatcg accagggcgg caagacgctg taccgcgtcc
     1081 gccttggacc gtacggccag ctcgacgaga tcaaccgcac ccgcaaactg ctggccgaga
     1141 acagcatcga ggcgcaggtc gtccgactca agtagcagtc agcgaacttc gccgccgttg
     1201 cgttatccaa acggcaccca acgattcatc cgctggagaa ccgacatgca tttgatcctt
     1261 gggcggcgcg agttcctcgc cgccgcagtg gcgctgccga ccatcgcgct ggcgcaggcc
     1321 ggcaagaagc ccgacgccgg cattgattac aacgagctgc ggccggcgca gccgaccgaa
     1381 gcgccgggca agatcgaggt actcgaattc ttctggtacg gctgcccgca ctgcgccgcg
     1441 ttcgatccgg aactggcagc gtggcgcaaa cgtctgtcag ccgacgtcgc ctatcgccgc
     1501 aacccggtcg cgttcagcga cgcgcagacg ccccacacca agatcttcta cacgctggag
     1561 gcgctcggca aggtggacgc gctgcactcg cgcgtgttcg ccgagatcgt cggcagccgc
     1621 aaaccgatgc tgaagaccga cgacatcgcc gatttcatgg ccgccaacgg catcgaccgc
     1681 aaacagtggc tcgacacgtt caattccttc accgtcgcta cacgcgcgtc gcgcgccgcg
     1741 caggtgtggc gcgcgtacaa ggtcgacggc acgccgacca tcggcgtcga cggccgctac
     1801 accacgtcgc cggcgcaggc caacggtcgc gccgaatgcc tgcgcgtggt cgatttcctg
     1861 atcgaccgca tccgcgcgga gaagggcggc gtcgcgaaga agtagtcgca cgctgcgacc
     1921 gcatcagaca gcccgcgctg cgggccgtcg tcgctcgtgg accggccgca gccaccgctg
     1981 cgtaaactgc agcggatgtc attcaacgtc ttcatcaccg gcgcctccag cggcatcggt
     2041 gccgctctgg cgagcgagtt cgcacgccgc ggtgccgcgg tcgggctggt cgcgcggcgc
     2101 aaggcggcac tcgacgagtt gatcggcagg ctgcccggcc gccacttcgc gtatgccgcc
     2161 gacgtgaccg acaaggacgc gctgatcgcg gccgcacacg cgttcgagcg cgatagcggc
     2221 ggcgtcgaca tcgtggtcgc caacgccggc atttccgtcg gcgtgctgac cgagcactac
     2281 gaagatctcg cggcgttcaa gcaggtgttc gacaccaacg tgctggcgat ggcgcacaca
     2341 ttccatccgt tcatcgcgac gatgcgcgaa aggcgtcgcg gcacgctggt cggcatcgcc
     2401 agcgtcgcgg gcatccgggg gctgcccggc tccgaggcgt actgcgcatc gaaggccgcg
     2461 gcgatcagct actgcgaaag cctgcgcgtg gaactgaagc gcttcggcgt gcgcgtggtg
     2521 accctcgcac ccggcttcat ccgtacgccg ctgaccgcca ggaatccgta ccggatgccg
     2581 tttctgatgg agcccgacgc attcgcgcgc cgggccgtcg acgccatcat cgcgggcgtc
     2641 agctaccgcg tgattccatg gcagatgggc ctggccgcca aactgctgcg cgcgctgccg
     2701 aactgggcgt tcgacaagct gatgtcgaac cggccgtaca agccgcgcaa gaccgaaccc
     2761 cggccatgac gtggtgggac gccgtcgggc aggcgcatgc cgcggccgcg ccgggctttc
     2821 gcatcgtatc gctggtgccg tcgctgaccg agttgctgct cgatctcgac ctgcgcgagc
     2881 acgtcgtcgg ccgcaccggc ttctacatcc atccacgcga actggtgcgt gccattccga
     2941 aggtcggcgg caccaaggac gtcaagctcg agcgcgtgcg cgaactcgaa ccgacccacg
     3001 cgatcgtcaa tatcgacgag aacgagctgc cgaccgtgca agcgctgcgc gaattcgtgc
     3061 cgcacgtgat cgtctcgcat ccgcaggcgc cggaagacaa tcccgcgctg tatcgactcc
     3121 tcggaggcgt gttcggccgc gcgccggagg ccgagcggct gtgcgcggat cttcagcgcg
     3181 aactcgacgc ctgccgcgca atcgactggg cacgcgagcg agtgctgtat ctgatctgga
     3241 aggatccttg gatgacgatc gccgccgaca cctacatcgc acgcacgctg gcgcgggtgg
     3301 ggtgggacgt ggtgcgcggt cccggcggcg agcgcggcgc ggcgcgctat ccggtcatcg
     3361 acgatctcga cgtcgaggtc ggacgcgtcg accgcgtgct gctgtcgacc gaacccttca
     3421 tgtttcgcgc gccgcacgtc gctgcgttgc gcgcacgctt tgcgggcaag tgcgtcgagt
     3481 tgatcgacgg tgaaatgacg agctggtacg gctcgcgggc gatcgagggg ctgcgattcc
     3541 tgcgccgaca ccgcgcggcg cgggcaccct gatcaacgtt gcgcttggcg ggcgacccgc
     3601 aggacgcccg ccgcacacgc cggtcagatg ttcggcgaag caaggtaggc ggcaatgtcg
     3661 gcaaggtccg acgctgagaa cttcccgctg aactgaccca tgccgccctt gttggccgct
     3721 atggtgctgg cgatcagcgt cgggttgttc g
//
LOCUS       DBNHBY010000483         5262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_436820, whole genome shotgun sequence.
ACCESSION   DBNHBY010000483 DBNHBY010000000
VERSION     DBNHBY010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_436820"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16HB_11925"
     CDS             complement(<1..706)
                     /locus_tag="AC16HB_11925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR5607543.1"
                     /translation="MIHVAAKVLLGPVIYAQAQRVRATAIELPEPPGPREGVEGRGSV
                     ALRLLVAGDSSAAGVGARMQEEALALPLARELARRLHGAVRWQLIARTGLTSEGVLHL
                     MMHGHVHAADVAVVVVGVNDITKEVPLQQALRKRGEIAAWLRTHVGASHVVFPATPDM
                     DLFPALPQPLAWLAGKLSKRNNAAQARWARTLPGVSHVAMDGVTRSELFAADGFHPAP
                     ALYARVRARLALHIEAI"
     gene            complement(729..1166)
                     /locus_tag="AC16HB_11930"
     CDS             complement(729..1166)
                     /locus_tag="AC16HB_11930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5607544.1"
                     /translation="MRLRPEITLDALNARGAGRLPGLFGLQVLHIDERELLMRLPIRD
                     ELLAPNGFLHAASVIALADTACGYACVAHLPAGAENFTTVELKANFLGTTTGGAIDAA
                     ARAAHLGRTTHVWDATVTEVESGKTIALFRCTQMILWPKSGKR"
     gene            complement(1174..1941)
                     /locus_tag="AC16HB_11935"
     CDS             complement(1174..1941)
                     /locus_tag="AC16HB_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR5607545.1"
                     /translation="MTIKSESAEGVCAIEIARPEKKNAITVGMYSPLAQAIAAAHDDP
                     AVRAILIHGQPDIFTAGNDLDDFLKNPPREGMDAPVFRFMQALGYAEKPVVAAVNGAA
                     VGIGTTMLLHCDLVYAADNAMFSMPFVSLGLCAEFASSLLVPLAAGYHKAAEKLLLAD
                     PISAEEAVEMKIVNRILPPNEVLAYARKQAARFAALPPNSVRETKRLMRAGWKALVEK
                     TIVDEATTFGRMLQSAEAKEAFTAFFERRKPDFSKFS"
     gene            complement(1938..2408)
                     /locus_tag="AC16HB_11940"
     CDS             complement(1938..2408)
                     /locus_tag="AC16HB_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5607546.1"
                     /translation="MVADDFYQAFADRDWRTMGALYADDAIFSDPAFSSLNAREVRAM
                     WRMLITRGTDLAVEYEVLQQGADAARVRWTAHYTFSQTRRPVVNVITATMKLRDGRIV
                     EHVDAFDFHRWARQALGAPGLLLGWTPFFQRKVQGQARAGLRQFMQRIEKETSP"
     gene            complement(2412..2645)
                     /locus_tag="AC16HB_11945"
     CDS             complement(2412..2645)
                     /locus_tag="AC16HB_11945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5607547.1"
                     /translation="MAAAGEIDRRALLERGFADAPFVAENGIRLADIGPGWCEATVDI
                     TPHLLQQSGVVHAGLLTTLADHCAGAWLSKYAG"
     gene            complement(2650..3849)
                     /locus_tag="AC16HB_11950"
     CDS             complement(2650..3849)
                     /locus_tag="AC16HB_11950"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR5607548.1"
                     /translation="MTRQVQDAYIVAATRTPIGKAPKGSFRNLRPDDLLVAAIRGALA
                     QVPSRDPKAVEDAIIGCAMPEAEQGLNVARIAVLLSGLPNSVGGMTINRFCSSGLNAV
                     AIAADRIRVGEADVMIAGGAESMSMVPMTGNKPSFNTHIFERDENIGIAYGMGLTAEK
                     VATQWKVTREMQDEFAYQSHMKAIAGQKAGEFKDEITSIDVIEKFPNLAKRDVDVKSR
                     KIDLDEGPRADTNLEALAKLKPVFAAKGSVTAGNSSQTSDGAGALILASEKAVKQFNL
                     SPLARFVSFAIRGVPPHIMGIGPKEAIPVALRLAGLKAEDMGWIELNEAFAAQALAVI
                     NDLGLDKTKVNPIGGAIALGHPLGATGAIRSATVVQALRRRNLKYGMVTMCVGTGMGA
                     AGIFERV"
     gene            complement(3961..>5262)
                     /locus_tag="AC16HB_11955"
     CDS             complement(3961..>5262)
                     /locus_tag="AC16HB_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5607549.1"
                     /translation="DVDFALRWGFGWQTGPFETWQAAGWKQIAEWVQSDIDKGDALAK
                     TPLPKWVFEGPVAKAGGVHTKGGSWSVAKKRFVGRSELPVYSRQIFRAPLAGEGAPTG
                     HTAGSTLHEDDSGRIWKLDAGLASEVLVFSIKSKMHAIGPGVLAGLTKAIELAEQSYR
                     GLVIWSPDEPFSVGADLNAMLPVFMSGGAKAIAPEVAKFQAAMMRVKYAQVPVVAAVS
                     GMALGGGCELALHSAKRVANLESYIGLVEVGVGLIPAGGGLKEGALRAARAAQAAGQT
                     DVFSFVRNWFTNAAMAKVATSAHEARAMGYLLPSDTIVFNSHELLWTAIGEAFALHAT
                     GYRPPLAPKGFPVAGRTGVATIKGQLVNMRDGGFISQHDFHIASLIADVMCGGDVEPG
                     SLVDEQWLLDLERTHFVSLVNHPKSQERMMGMMQTGKPVRN"
BASE COUNT          831 a         1872 c         1686 g          873 t
ORIGIN      
        1 cgatggcttc gatatgtagt gccagccgcg cacgcacgcg tgcgtacagc gccggcgccg
       61 gatgaaaacc gtcggcggcg aacagttcgg atcgcgtgac accgtccatc gcgacatgcg
      121 acacgcccgg tagcgtccgc gcccagcgcg cctgcgccgc attgttgcgc ttcgacagct
      181 tgccggccag ccacgcgagc ggctgcggca gcgccgggaa caggtccatg tccggcgtgg
      241 ccggaaacac gacgtgcgac gcgcccacgt gcgtgcgcag ccacgctgcg atctcgccgc
      301 gcttgcgcag cgcctgctgc agcggcactt ccttcgtgat gtcgttgacg ccgaccacga
      361 ccaccgcgac atcggcggca tgaacgtgac catgcatcat caagtgcagc acgccttcgg
      421 acgtgagccc agtccgcgcg atcaactgcc agcgcaccgc gccgtgcaac cgccgcgcga
      481 gttcgcgcgc cagcggcagc gccagcgcct cttcctgcat ccgcgcgccg acgcccgccg
      541 cgctggagtc gccggcgacc agcagccgca gcgcgacgga accgcggccc tcgacgccct
      601 cgcgcggacc gggcggctcc ggcagttcga ttgcggtcgc acgcacccgc tgcgcctgcg
      661 cgtagatcac cggacccagc agcaccttgg cagcgacgtg gatcatctcg atcgactgca
      721 gcgccgtttc agcgcttgcc cgacttcggc cacaggatca tctgcgtgca gcggaacagc
      781 gcgatcgtct tgccgctttc gacctcggtc acggtcgcgt cccagacgtg cgtggtgcgg
      841 cccagatgcg ccgcgcgtgc ggcggcatcg atcgcgccgc cagtggtggt gccgagaaag
      901 ttcgccttca attccacggt ggtgaagttc tcggcacccg caggcaggtg cgcgacgcag
      961 gcgtagccgc acgcggtatc cgccagcgca atcacgctgg ccgcgtgcag aaatccgttc
     1021 ggcgccaaca gttcgtcgcg gatcggcagc cgcatcagca gttcgcgctc gtcgatgtgg
     1081 agcacctgca ggccgaacag ccccggcagc cggccggcgc cgcgcgcatt cagcgcgtcg
     1141 agcgtgatct cggggcgcag ccgcatcggt gcctcaagag aacttggaga aatccggctt
     1201 gcggcgctcg aagaacgcgg tgaaagcctc cttggcctcc gcgctctgca gcatgcgacc
     1261 gaaggtggtc gcctcgtcga cgatggtctt ctcgaccaga gccttccagc ccgcgcgcat
     1321 caaccgcttg gtctcgcgca ccgagttcgg cggcagcgcg gcgaagcgcg ccgcctgctt
     1381 gcgcgcatac gcgagcacct cgttcggcgg caggatacgg ttgacgatct tcatctcgac
     1441 cgcttcctcc gcgctgatcg gatcggcgag cagcagcttc tccgccgcct tgtgatagcc
     1501 ggcagcgagc ggcaccagca acgacgacgc gaattcggca cacaggccca gcgacacgaa
     1561 cggcatcgag aacatcgcgt tgtcggccgc gtacacgagg tcgcagtgca gcagcatcgt
     1621 ggtgccgatg ccgaccgccg caccgttgac cgcggcgacc acgggcttct cggcgtagcc
     1681 gagcgcctgc atgaacctga acaccggcgc gtccattcct tcgcgcggcg ggttcttcag
     1741 gaaatcgtcc aggtcgttgc cggccgtgaa gatgtccggc tggccgtgga tcaggatggc
     1801 gcgcaccgcg ggatcgtcgt gcgcggcggc gatcgcctgc gcgagcggcg aatacatccc
     1861 gaccgtgatc gcattcttct tttcggggcg cgcgatttcg atcgcgcaga cgccctcggc
     1921 actctcgctc ttgatcgtca tggggatgtc tccttttcaa tgcgctgcat gaactggcgc
     1981 agccccgcgc gggcctggcc ctgcaccttg cgctgaaaga acggagtcca gcctagcagc
     2041 aggcccggcg cgccgagcgc ctgccgtgcc cagcggtgga agtcgaacgc atcgacgtgc
     2101 tcgacgatgc ggccgtcgcg cagcttcatc gtcgcggtga tcacgttgac caccggccgg
     2161 cgcgtctgac tgaacgtgta atgcgcggtc cagcgcacgc gcgccgcgtc ggcgccctgt
     2221 tgcagcacct cgtactcgac ggcgagatcg gtgccgcgcg tgatcagcat ccgccacatc
     2281 gcgcgcacct cgcgcgcgtt caggctcgag aaggccgggt cgctgaagat cgcgtcgtcg
     2341 gcgtacagcg cgcccatcgt gcgccagtcg cgatcggcaa acgcctggta gaagtcatcg
     2401 gcgaccatcg gctaccccgc atacttcgac agccacgcgc ccgcgcagtg atcggccagc
     2461 gtcgtcagca ggccggcgtg cacgacgccg ctctgctgca ggagatgcgg cgtgatgtcg
     2521 accgtcgcct cgcaccagcc ggggccgatg tcggcgaggc gaatgccgtt ttctgcgacg
     2581 aacggcgcgt cggcgaagcc gcgctcgagc aacgcgcgac gatcgatttc gccggccgcc
     2641 gccaccggat cagacccgct cgaagatccc ggcggcgccc atgccggtgc cgacgcacat
     2701 cgtcaccatg ccgtacttga gattcctccg gcgcagcgcc tgcacgacgg tcgccgagcg
     2761 gatcgccccg gtggcgccga gcgggtggcc gagtgcgatc gcgccgccga tcgggttgac
     2821 cttcgtcttg tcgaggccga ggtcattgat caccgccagc gcctgcgccg cgaacgcctc
     2881 gttcagttcg atccagccca tgtcctcggc cttgagcccc gcgagcctga gcgccacggg
     2941 aatcgcttcc ttcgggccga tgcccatgat gtgcggcggc acgccgcgga tcgcgaacga
     3001 cacgaagcgc gccagcgggg acaggttgaa ctgcttgacc gccttttcgc tggcgaggat
     3061 cagcgcgccg gcaccgtccg acgtctgcga gctgttgccg gcggtgacgc tgcccttggc
     3121 cgcgaacacg ggtttgagct ttgccagcgc ttcgaggttg gtgtcggcgc gcgggccctc
     3181 gtcgaggtcg atcttgcgcg acttcacgtc gacgtcgcgc ttggcgaggt tggggaactt
     3241 ctcgatcacg tcgatcgacg tgatctcgtc cttgaactcg ccggccttct gaccggcgat
     3301 cgccttcatg tgcgactggt aggcgaactc gtcctgcatc tcacgcgtga ccttccactg
     3361 cgtggccacc ttctcggccg tcaggcccat gccataggcg atcccgatgt tctcgtcgcg
     3421 ctcgaagatg tgcgtgttga acgacggctt gttgccggtc atcggcacca tgctcatcga
     3481 ctcggcgccg ccggcgatca tcacgtcggc ttcaccgacg cggatacggt cggccgcgat
     3541 cgccacggca ttcagccccg acgagcagaa gcggttgatc gtcatgccgc caaccgagtt
     3601 cggcagcccg gacagcagca cggcgatgcg cgcgacgttc agcccctgct ccgcttccgg
     3661 catcgcgcag ccaatgatcg cgtcctcgac ggccttcgga tcgcgcgacg gcacctgcgc
     3721 cagcgcgccg cggatcgccg cgaccagcag gtcgtccggc cgcagattcc tgaagctgcc
     3781 cttcggcgcc ttgccgatcg gcgtgcgcgt ggcggcaacg atgtatgcat cctgtacttg
     3841 gcgagtcatt tctgctcctg cattaccgaa tcggtttcag ttgccggggg atccaagggg
     3901 gctggcaagc ccccttgcgt cctgacctga gagatgcaat cggaaacgcg ggccgtaacg
     3961 tcagttacgg accggctttc cggtttgcat catgcccatc atccgttcct gcgacttcgg
     4021 atgattgacg agcgaaacga aatgcgtgcg ctccagatcg agcagccact gctcgtcgac
     4081 cagcgaaccc ggctcgacgt cgccgccgca catcacgtcg gcaatcaggc tggcgatgtg
     4141 gaagtcgtgc tggctgatga agccgccgtc gcgcatgttg accaattgcc ccttgatcgt
     4201 cgcgacaccc gtgcgtcccg cgaccggaaa gcccttgggt gcaagcggcg gccgatagcc
     4261 cgtcgcgtgc agcgcgaacg cctcgccgat cgcggtccac agcagttcat ggctgttgaa
     4321 aacgatcgtg tcgctcggca gcaggtagcc catcgcgcgc gcctcgtgcg cagaggtcgc
     4381 gaccttggcc atcgccgcgt tggtgaacca gttgcggacg aacgagaaca cgtcggtctg
     4441 ccctgcggcc tgagcggccc gtgccgcgcg caacgcgcct tccttgagcc cgccgccggc
     4501 gggtatcaag cccacgccga cctcgacgag gccgatgtag ctctcgaggt tcgccacgcg
     4561 cttggccgag tgcagcgcaa gttcgcagcc gccaccgagc gccatgccgc tgaccgcggc
     4621 aacgaccggc acctgtgcat acttcacgcg catcatcgcg gcctggaact tcgcgacctc
     4681 gggcgcgatt gccttcgcgc cgccggacat gaacaccggc agcatcgcgt tcaggtccgc
     4741 gccgaccgaa aatggctcgt caggcgacca gatgaccagc ccgcgatacg actgctcggc
     4801 aagttcgatc gccttggtca gccccgccag cacgcccggg ccgatcgcgt gcatcttcga
     4861 cttgatcgag aacaccagca cttcgctcgc cagccccgcg tccagcttcc agatacgacc
     4921 cgagtcgtct tcgtgcagcg tggagccggc ggtgtggccg gtcggcgcac cttcgcccgc
     4981 gagcggagca cggaagatct gccgcgaata gacgggcagc tcggagcggc cgacaaaccg
     5041 cttcttcgcc accgaccacg aaccgccctt cgtgtgcacg ccgcccgctt tcgcgaccgg
     5101 tccttcgaac acccacttcg gcagcggcgt ctttgccagc gcgtcgccct tgtcgatgtc
     5161 ggactgcacc cattccgcga tctgcttcca gcctgcggcc tgccaggttt cgaatggacc
     5221 ggtctgccag ccgaaacccc agcgcagtgc gaagtcgacg tc
//
LOCUS       DBNHBY010000484         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1452080, whole genome shotgun sequence.
ACCESSION   DBNHBY010000484 DBNHBY010000000
VERSION     DBNHBY010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1452080"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="AC16HB_11960"
     CDS             <1..1279
                     /locus_tag="AC16HB_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5607550.1"
                     /translation="EFTPRRVAELCGISAQEVEALAHDYWHIRPAAIRLNYGMQRVAG
                     GGNAVRAVACLPALAGHWRDPAGGVLLSTSGAFHIDAERLYRPELLAGRAPRTINMAT
                     IGHDLAHADPPIDALIVYNSNPVAVAPNSTAVAAGFAREDLFTVMLEHFLTDTADYAD
                     YVLPATTQLEHVDVVKPYGHLYVMANNAAIEPVGEALPNTEIFRRLAARMGFIEPCFA
                     ESDEEIAAQAFRQVGTTAHYDWKVASAQGWQRLDVPQRYAPFENGNFPTPSGKCEFYS
                     RTLAAQGLDPLPSFVPPHEGPRSNRALAQRFPLALISPPARNFLNSTFVNVQSLRDTE
                     GEPHLEIHPDDAVPRGIADAERVRVYNDRGSFELTARITDRARPGVVVALSIWWKKLA
                     RDGRNANEVTSPALTDIGRAPTFYDCLVQVERG"
     gene            1435..1872
                     /locus_tag="AC16HB_11965"
     CDS             1435..1872
                     /locus_tag="AC16HB_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607551.1"
                     /translation="MTPLATYLVEDSRVIRESLTAALEEMVSLRVVGAAEDESTAVSW
                     LADAANRCDLVVVDLFLKRGSGFGVLEAARREGRSCALVVLTNYATPDIRRRCLSLGA
                     DRVFDKSDEIDGLIDFCIERARVAPAAADAPAPIGTKPTASGA"
     gene            complement(1874..2506)
                     /locus_tag="AC16HB_11970"
     CDS             complement(1874..2506)
                     /locus_tag="AC16HB_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607552.1"
                     /translation="MIRIGIVDDHAIVRSGLRQYLSEHADLQVTGEAANGREAIDLVR
                     TGDIDVLLLDLSMPEHDGISALAAIKARAPDLPILILSGFAEAHYATTLLKQGASGYL
                     NKECDPAEIANAIRTVHRGRKYITPGVAELLADALESKGDQAPHQQLSEREFQVFLRL
                     AKGETIGHMAESMSLSVKTVSTYRTRVMEKLKLASNSDLTYYALKNGLIQ"
     gene            2477..2659
                     /locus_tag="AC16HB_11975"
     CDS             2477..2659
                     /locus_tag="AC16HB_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607553.1"
                     /translation="MVVNDANSDHLSRWTEGNEEPSLDVRQVNENRLVVEIDPSALGL
                     FVAVVAVLLAISASAS"
BASE COUNT          456 a          922 c          904 g          492 t
ORIGIN      
        1 cgagttcacg ccgcggcgcg tcgcggaact gtgcggcatc agcgcgcagg aggtcgaagc
       61 gctcgcgcac gactactggc acatccggcc ggcggcgatc cgcctgaact acggcatgca
      121 gcgcgtcgcc ggcggcggca acgcggtgcg cgcggtcgcg tgcctgcccg cgctcgccgg
      181 tcactggcgc gatccggccg gcggcgtgct gctgtccacc agcggcgcgt tccacatcga
      241 cgccgaacgc ctgtatcgcc ccgagctgct cgccgggcgc gcgccgcgca cgatcaacat
      301 ggccacgatc ggccacgacc tcgcgcacgc cgacccgccg atcgacgcgc tgatcgtcta
      361 caacagcaat ccggtcgcgg tggcgccgaa ctcgacggcg gtggcggcgg gcttcgcgcg
      421 cgaggatctc ttcaccgtca tgctggaaca cttcctgacc gacacggccg attacgccga
      481 ctacgtgctg cccgccacca cgcaactgga gcacgtcgac gtcgtcaagc cgtacggcca
      541 cctgtacgtg atggcgaaca acgccgcgat cgagccggtc ggcgaagcgc tgcccaacac
      601 cgagatcttt cgccggctgg ccgcgcggat gggcttcatt gagccgtgct tcgccgagag
      661 cgacgaggag atcgccgcgc aggcgttccg ccaggtcggc accaccgccc attacgactg
      721 gaaggtcgca agcgcgcagg gctggcagcg gctcgacgtg ccgcagcgct acgcaccgtt
      781 cgagaacggc aacttcccga cgccgagcgg caagtgcgag ttctattcgc gcacgctggc
      841 tgcgcagggg ctcgatccgc tgccgagctt tgttccgccg cacgaagggc cgcgcagcaa
      901 ccgcgcgctt gcgcagcgct ttccgctggc gctgatctcg ccgcccgcgc gcaatttcct
      961 caacagcacg ttcgtcaacg tgcaaagcct gcgcgacacc gaaggcgagc cgcatctgga
     1021 gatccacccg gacgatgcgg tgccgcgtgg cattgccgat gcggaacggg tgcgtgtgta
     1081 caacgatcgc ggctcgttcg aactcaccgc gcgcataacc gatcgtgcgc ggcccggcgt
     1141 ggtggtggcg ctgtcgatct ggtggaagaa gctcgcgcgc gacggcagga acgccaacga
     1201 agtcaccagc ccggcactga ccgacatcgg ccgcgcgccg acgttttacg actgcctggt
     1261 acaggtcgaa cgcggctgac cgagaggtga tggccgagac gcggctgatt tcgggtagga
     1321 cgattcctac cttcccgcgc ggatcagccc gatggacttt ccggcggttc gctgcatgat
     1381 gtggtcgggg ccattccgca ccgtaacggt tgccgtcact tcccgcactc gtccatgacg
     1441 ccgctcgcga cctatctggt ggaagacagt cgtgtcattc gcgagagcct gacggccgcc
     1501 ctggaggaaa tggtgtcatt gcgggtcgtg ggtgccgcag aagacgaatc gaccgcagtg
     1561 tcctggttgg cagacgcggc gaatcggtgc gatctcgtgg tcgtcgacct ctttctcaag
     1621 cgcggatcgg ggttcggggt gttggaagca gcgcgccgtg agggccgatc ctgcgccctg
     1681 gtcgtgctta ccaattacgc gacgcccgac attcggcgcc gctgcctgtc actcggtgcc
     1741 gatcgagtat tcgacaagtc agatgaaatc gacgggctga tcgatttctg catcgagcgt
     1801 gcccgcgtcg cgccggccgc cgcggacgcg ccggcaccga tcggcacgaa gcccactgca
     1861 agcggcgcct gattcactga atgagcccgt tcttgagggc gtagtaggtc agatcgctgt
     1921 tcgacgcgag tttcagcttc tccatgacgc gcgttcgata agtgctgacc gtcttcacgc
     1981 tcagcgacat gctctcggcc atgtgaccaa tggtttcgcc tttcgccagt cgcaggaaga
     2041 cctgaaactc gcgttcggac aactgctgat gcggtgcctg atcgcccttg ctttcgagcg
     2101 catcggccag cagttccgcc acacccggcg tgatgtactt gcgcccccga tgcacggtgc
     2161 ggatcgcgtt cgcgatctcc gcaggatcgc actccttgtt caggtagccg ctcgcgccct
     2221 gcttgagcaa cgtggtggcg taatgcgcct cggcgaagcc gctcaggatc aggatgggca
     2281 ggtcgggtgc gcgcgccttg atcgcagcca gcgcgctgat gccgtcgtgc tccggcatcg
     2341 acaggtcgag cagcagcacg tcgatgtcgc ccgtgcgcac cagatcgatc gcttcgcgcc
     2401 cgttggcggc ttcaccggtg acctgcagat cggcgtgctc cgacaaatac tgtcgtagac
     2461 cggagcgcac gatcgcatgg tcgtcaacga tgccaattcg gatcatctgt ctcgatggac
     2521 cgaagggaac gaggaaccga gtctagatgt acggcaggtc aatgaaaatc gcctcgttgt
     2581 tgaaattgac ccgtcggcgc tcgggctctt cgtcgcagtg gtcgcggtcc tgctcgccat
     2641 cagcgcgagc gcgtcctgag tggtgtggcg tgtcggtggc gtcctacagg acgttggccg
     2701 tctgaccgac atcacgcagg ctgctcgtcc catggcgagc ggcggcacct cttcataagc
     2761 tttcctcatc gcag
//
LOCUS       DBNHBY010000485         3297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_572484, whole genome shotgun sequence.
ACCESSION   DBNHBY010000485 DBNHBY010000000
VERSION     DBNHBY010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_572484"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16HB_11980"
     CDS             <1..633
                     /locus_tag="AC16HB_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HIR5607554.1"
                     /translation="RALRAKSASGPVLLLDHGDNVMSGGTCDTTDVLEECLRQGMSKI
                     GVGPTCDPVAVAIAATAGVGDRVKMPVGNVRPLGLGGDPRPRFVIDARVRAVTDGRFR
                     ITGPIYTGETWSMGRTVVLQADAFTIVVTERPMEPLDLGVFESAGVDPRAFDYLILKS
                     RMYCRPSFVPISSGLIECDSRGVCSSDYSLFRFRHVRRPIYPLDAGTPWR"
     gene            630..2006
                     /locus_tag="AC16HB_11985"
     CDS             630..2006
                     /locus_tag="AC16HB_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5607555.1"
                     /translation="MNIDPFATLAELSAVLARRDASALELTELYLARIARANAKLNAF
                     VSVDAEIARRHARASDERRAGGLTLGPLDGLPIALKDLLEIEGRVTTVGSALWRDRRS
                     AITAPVVERLLAAGMVPLGKTQMVEFAFGTWGTNPHLGTPCNPWDHAVHRIPGGSSSG
                     SGVAVAAGLAPAAIGSDTGGSVRIPAALNGITGLKTTGGLISTRGAFPLSTTLDTIGP
                     MTRSAEDAALLTAVMADARSPGLTAPAADAVGVRIDVLPVDQYPIAVEPAVNAALTAA
                     QQTLRALGAHLVERRFPFDFSELALRNGRLTAAEGFAVHRAYIEDEQRPIGPSVRARM
                     LGGKAISAADYIDALAYRRAAAATWREWMRDTDALLTPTLPMAACPVDAVDEAVAPLA
                     SFTRAGNFLDACGLSLPAGFSDDGLPIGVQLLGKPFDEATLLRIGRAFQQATDWHRRR
                     PDLGALFD"
     gene            2021..>3297
                     /locus_tag="AC16HB_11990"
     CDS             2021..>3297
                     /locus_tag="AC16HB_11990"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIR5607556.1"
                     /translation="MNLAELDKLQSLHPYTNLRKHEACGPMIITRGRGIHVYDTEGKE
                     YIEGLAGLWCASLGFSNERLIDAATRQLNRLPYYHAFAHKTSDVVIEATRDLLAIAPG
                     RMARVFFGTSGSDANDTAIKLVRYYNNALGRPHKKRIIARTRAYHGVSIAAASLAGLP
                     HLHTGFDLSTADVVRAECPHHYRFAQMGESEEAFAARLARDLERQIEQEGPGTIAAFI
                     AEPVMGAGGVIPPPAGYFDMIQPILRQHDILFIADEVICGFGRTGQMWGSVTYDIEPD
                     IVTCAKALTGGYVPFSATMISQRVYEPIAEQAAKIGMFGHGYTYSAHPLGAAVAVEAM
                     KIYREVDIAARVRSLAPRFQDGLRALAASPIVGEVRGVGLIAAVEFVKNKQTRESFSP
                     TVAVAAHFTDRAQAHGLLVRQLGEAVALCPPLI"
BASE COUNT          497 a         1145 c         1156 g          499 t
ORIGIN      
        1 cgcgcgctgc gcgcgaagag cgcaagcggc cccgtgctgc tgctcgacca cggcgacaac
       61 gtgatgagcg gcggcacctg cgacacgacc gacgtgctcg aggagtgcct gcgccaaggc
      121 atgtcgaaga tcggcgtcgg gccgacctgc gacccggtcg cggtcgcgat cgccgcgacc
      181 gccggcgtag gtgaccgcgt gaagatgccg gtcgggaacg tgcggccgct cggcctcggc
      241 ggcgacccgc ggccgcgctt cgtgatcgat gcgcgcgtgc gcgcggtgac cgacggccgc
      301 ttccgcatca ccggcccgat ctacaccggc gagacctggt cgatgggccg cacggtcgtg
      361 ctgcaggccg acgcgttcac gatcgtcgtc acggagcggc cgatggagcc gctcgacctc
      421 ggcgtgttcg aaagcgccgg cgtcgatccg cgtgcgttcg attacctgat cctgaagtcg
      481 cgcatgtact gccggccgag cttcgtgccg atttcgagcg ggctcatcga gtgcgacagc
      541 cgcggcgtct gcagctcgga ctattcgttg ttccggttcc ggcacgtgcg ccggccgatc
      601 tatccgctcg atgcggggac gccgtggcga tgaacatcga tccgtttgcg acgctggcgg
      661 agctttccgc ggtgctggcg cggcgcgacg cgagcgcgct ggagctgacg gagctgtacc
      721 tcgcgcgcat cgcgcgcgcc aacgccaagc tcaacgcgtt cgtcagcgtc gacgcggaga
      781 tcgcgcggcg ccatgcgcgc gcgtccgacg agcgccgcgc cggcgggctc acgctcggcc
      841 cgctcgacgg actgccgatc gcgctgaagg acctgctgga gatcgagggc cgtgtaacga
      901 cggtcggctc ggcactgtgg cgcgaccgcc gcagcgcgat caccgcgccg gtagtcgagc
      961 ggctgctcgc ggccggcatg gtcccgctcg gcaagacgca gatggtcgag ttcgcgttcg
     1021 gcacctgggg cacgaatccg cacctgggca cgccgtgcaa tccgtgggat cacgcggtgc
     1081 atcgcatccc cggcggctcg tcgagcggct cgggcgtggc ggtcgcggcg gggctggcgc
     1141 cggctgcgat cggctccgac accggcggct cggtgcgcat ccccgccgcg ctgaacggca
     1201 tcacggggct gaagaccacc ggcgggctga tcagcacgcg gggcgcattc ccgctgtcga
     1261 ccacgctcga cacgatcggg ccgatgaccc gcagcgccga ggacgccgcg ctgctgaccg
     1321 cggtgatggc cgatgcgcgc tcccccggcc tgacggcgcc cgcggccgat gcggtcggcg
     1381 tgcgcatcga cgtgctgccg gtcgaccagt acccgatcgc ggtcgagccc gcggtgaacg
     1441 cggccctgac ggccgcgcag cagacgctgc gcgccctcgg cgcgcacctg gtggagcggc
     1501 gcttcccgtt cgacttctcc gaactcgcgc tccgcaatgg ccggctcacc gccgccgagg
     1561 gtttcgcggt gcatcgcgcg tacatcgagg acgagcagcg gccgatcgga ccgagcgtgc
     1621 gcgcgcgcat gctcggcggc aaggcgatct ccgccgccga ttacatcgat gcgctcgcgt
     1681 accggcgcgc cgccgccgcg acctggcgcg aatggatgcg cgacaccgac gcgctgctga
     1741 cgccgacgct gccgatggcc gcatgcccgg tcgacgcggt ggacgaagcg gtcgcgccgc
     1801 tggcgagctt cacccgcgcc ggcaattttc tcgacgcctg cgggctgtca ctgccggccg
     1861 gcttctcgga cgatggcctg ccaatcggcg tacaactgct cggcaagccg ttcgatgaag
     1921 cgacgttgct tcgcatcggt cgtgcgttcc agcaggcgac cgactggcat cggcgcaggc
     1981 cggacctggg cgcactgttt gactgaacgc tggagacgcg atgaacctcg ccgaactcga
     2041 caagctgcaa tcgctgcacc cctacaccaa cctgcgcaag cacgaagcgt gcggcccgat
     2101 gatcatcacg cgcggtcgcg gcatccacgt ctacgacacc gaaggcaagg aatacatcga
     2161 gggcctggcg ggtctgtggt gcgcatcgct cggcttctcg aacgagcggc tgatcgatgc
     2221 agccacgcgg cagctcaacc ggctgcccta ctaccatgcc ttcgcgcaca agacgagcga
     2281 cgtggtcatc gaggccacgc gcgacctgct cgcgatcgct cccggcagga tggcgcgtgt
     2341 gttcttcggc accagcggct cggacgcgaa cgacacggcg atcaagctcg tgcgctacta
     2401 caacaacgcg ctcggccgcc cgcacaagaa gaggatcatt gcgcgtaccc gtgcctacca
     2461 tggcgtgagc atcgccgctg caagcctggc ggggctgccg caccttcaca ccggcttcga
     2521 cctgtcaacc gcggacgtgg tgcgcgccga gtgtccgcat cactaccgct tcgcccagat
     2581 gggcgagagc gaggaggcat tcgcggcgcg gctggcgcgc gatctcgagc ggcagatcga
     2641 gcaggaaggg cccggcacga tcgccgcgtt catcgccgaa ccggtgatgg gcgccggcgg
     2701 cgtgattccg ccgccggcgg gctacttcga catgattcag cccatcctca ggcagcacga
     2761 catcctgttc atcgcggacg aggtgatttg cggcttcggc cgcacgggtc agatgtgggg
     2821 cagcgtgacc tacgatatcg agcccgacat cgtgacgtgc gccaaggcgc tgacgggcgg
     2881 ctatgtgccg ttctcggcga cgatgatctc gcagcgggtc tatgagccaa tcgcggagca
     2941 ggcggcgaag ataggcatgt tcggacatgg ctacacgtat tcggcgcatc cgctcggcgc
     3001 ggccgtcgcg gtcgaggcaa tgaagatcta ccgcgaggtc gacatcgccg cgcgcgtgcg
     3061 ctcgctggcg ccgcgcttcc aggacggact gcgcgcgctg gctgccagtc cgatcgttgg
     3121 cgaagtgcgc ggcgtgggac tcatcgcggc cgtcgagttc gtgaagaaca agcaaaccag
     3181 ggaatcgttt tcgccgaccg tagccgtggc tgcacacttt accgaccgtg cgcaggcgca
     3241 tgggctgctc gtgcgccagc tcggcgaggc ggtcgcgctc tgcccgccgc tcatcat
//
LOCUS       DBNHBY010000486         4609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1046053, whole genome shotgun sequence.
ACCESSION   DBNHBY010000486 DBNHBY010000000
VERSION     DBNHBY010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1046053"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="AC16HB_11995"
     CDS             complement(<1..400)
                     /locus_tag="AC16HB_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607557.1"
                     /translation="MVKTMTKGTLAGKKLVHAAAKAPKVSKAAVRPQARTADNGSGAK
                     KAAALESGRELKAKGAPLAPVVDSSAKGRPGRKPKQQADTPMGGDGSADAVPEAGDDT
                     VDPSVQVVEKLSVKQRAKDRRAKEKALKEAL"
     gene            complement(784..2541)
                     /gene="dnaG"
                     /locus_tag="AC16HB_12000"
     CDS             complement(784..2541)
                     /gene="dnaG"
                     /locus_tag="AC16HB_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR5607558.1"
                     /translation="MIPQGFIQDLLARVDIVDVVGRHVQLKKAGQNYLGLCPFHGEKT
                     PSFTVSPSKQFYHCFGCGVHGTAVGFLMESRGLGFVEAVRELAQQVGMQVPEATGGRE
                     PSSKTRGLADALQQAADFYRRKLKESPAAIEYLKRRGLSGQTAARFALGFSPDGWQSL
                     REAFESYEDARLTEAGLVIVGDEGKRYDRFRGRVMFPIRNRRGAVIGFGARSIDGSEP
                     KYLNSPETPVFHKGQELYGLYEANDAIRKRGRAIVCEGYMDVIQLAQAGFEESVASLG
                     TAITSSHVGNLFRATDHVVFAFDGDAAGRKAARRALEAALIAMGDAKRASFLLLPEGE
                     DPDSLIKSHGAAGFETELARAMPLSAWFLRVISEGKLLDTAEGRAALAAEAKPLLAAM
                     PPGALRMQLIGEVATASRMAPADVELLFGLARWRRLPSAPLRRTQRPAPEVGDLKARV
                     LQWLVLFPELGREFNADIVRETDASEEPIDRKLREIWRVATQIPAATTGAVIQSVADS
                     EFLSEYEAIAAQGLAGDVDPAAARDEVAGAFARLNQQRLEARRAELLGRYQADPSEAN
                     LRAYQQADREYARLRAGGG"
     gene            complement(2553..3002)
                     /locus_tag="AC16HB_12005"
     CDS             complement(2553..3002)
                     /locus_tag="AC16HB_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR5607559.1"
                     /translation="MTLKERINEDMKAAMRAREAERLSAIRLLLAAVKQKEIDERIVA
                     DDAAVMTIVEKLIKQRRDSIAQFDKAGRQDLVAKETAELNLLASYLPQQMNDVELAAE
                     VDAAIAAASAGGPQAMGKVMALLKPRIAGRADMGKASALVKQKLAGG"
     gene            complement(3071..3283)
                     /gene="rpsU"
                     /locus_tag="AC16HB_12010"
     CDS             complement(3071..3283)
                     /gene="rpsU"
                     /locus_tag="AC16HB_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5607560.1"
                     /translation="MPTIRLKENEPFEVALRRFKRTIEKSGLLTELRAREFYEKPTAE
                     RKRKKSAAIKRHFKRLRSQMLPKKLY"
     gene            3428..4441
                     /gene="tsaD"
                     /locus_tag="AC16HB_12015"
     CDS             3428..4441
                     /gene="tsaD"
                     /locus_tag="AC16HB_12015"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5607561.1"
                     /translation="MLVLGIETSCDETGIALLDENSGLLAHAVHSQVDMHRAYGGVVP
                     ELASRDHIRRVLPLTDRVLADAKRSRADIDAIAVTIGPGLIGALLVGASIAHGLGAAL
                     RRPVIGVHHMEGHLLSPLLANPAPAFPFTALLVSGGHTQLLHVGSVGDYRLLGETVDD
                     AAGEAFDKTAQILGLGYPGGPAVSRLADFGTPGAVTLPRPMLHSGDLAFSFSGLKTAV
                     LTAARRIDSTCEQARADLARGFVDAVVDVLATKSMRALEASGAGTLVVAGGVGANVQL
                     RERLSDETRRIGATVYFPPPDLCTDNGAMIAVAGLARLKRGAFDGSGAFTVHPRWTLS
                     DAR"
BASE COUNT          719 a         1611 c         1455 g          824 t
ORIGIN      
        1 cgagcgcctc cttcagcgcc ttttccttcg cgcggcggtc cttggcgcgc tgcttgaccg
       61 agagcttctc gacgacttga acgctcggat cgaccgtgtc gtctcccgcc tccggcaccg
      121 cgtcggcgct gccatcaccg cccattgggg tgtcggcctg ctgcttgggt ttgcgtccgg
      181 ggcggccctt ggcggacgaa tcgacaaccg gagccagagg agcgcccttg gccttcaatt
      241 cacggccgga ctcaagcgcg gccgctttct tcgcccccga gccgttgtct gccgtcctcg
      301 cctgtggcct caccgccgcc tttgaaacct tgggagcctt cgctgccgca tgcaccagct
      361 tcttgcccgc aagggttccc ttcgtcatcg tcttgaccat ccagccaccc gatcaatgcc
      421 tgtccacgcc aggaagatgc cggcgcacgg cgccaatgca atccttccgg ctgcctgtga
      481 ttcagatttc gattgcaaat tcgtcgcggc gttcgccgcc ggtgcactgc ttcgccggca
      541 ggacgggatg cactccgcct gaccagcaaa ccacggcgag attcgccacc ttcgcttcca
      601 ctggcccacc ggccgaaggt actcagaccg tcactgcatg agcgcgactt cgcgatccgg
      661 ccgatcagcc catcccgggc gcgacccgct gtctgctctg gtcgccggag cgacccatgc
      721 cgcccgcggc ggcctcataa acaaatcatt atagcgaacc cggacgctgg tctgcgccgt
      781 ctttcacccg ccacccgcac gcaggcgcgc gtactcgcgg tcggcctgct ggtaggcgcg
      841 cagattggct tcggaggggt cggcttggta gcgcccaagc agttcggcgc gacgggcctc
      901 caatcgctgc tggttcagcc gcgcgaacgc cccagcgacc tcgtcgcgag cggccgcggg
      961 atcgacgtcg cccgccaggc cctgcgcggc gatcgcctcg tactccgaaa ggaactcgct
     1021 gtcggcgacc gactggatca ccgccccggt cgttgccgct gggatctggg tcgccacgcg
     1081 ccagatttca cgcagcttgc ggtcgatcgg ctcctcgctg gcatccgtct cgcgcacgat
     1141 gtcggcgttg aattcgcgcc caagctccgg gaacaggacc agccattgga gcacgcgcgc
     1201 cttgaggtca ccgacttccg gcgccggtcg ttgcgtgcgc cgcaacggcg ctgacgggag
     1261 ccggcgccaa cgcgccaagc cgaacagcag ttcgacgtcg gccggggcca tccgactcgc
     1321 ggtggccact tcgccgatca actgcattcg gagtgcgccg ggtggcatcg ccgccagcag
     1381 cggcttcgcc tcggctgcca acgctgcccg tccttcggcg gtgtcaagta actttccttc
     1441 cgatatcacg cgcaggaacc atgccgatag cggcattgcg cgcgccagtt cggtctcgaa
     1501 tcccgcagcg ccgtgcgact tgatcaagct gtcgggatcc tcgccctccg gcagcagcag
     1561 gaagctggcg cgcttcgcat cccccatcgc gatcagcgcg gcttcgagcg cacgccgtgc
     1621 cgcctttcgg cccgcggcgt cgccgtcgaa tgcgaacacg acgtgatccg tcgcgcgaaa
     1681 gaggttgccg acgtggctgg aggtgatggc agtgccgagc gacgcgaccg actcctcgaa
     1741 gccggcttgc gcgagctgga tcacgtccat gtagccttcg cacacgattg ctcggccccg
     1801 cttgcgtatc gcatcgttcg cctcgtacag cccgtacagt tcctgtccct tgtgaaagac
     1861 cggggtttcc ggcgagttca ggtacttcgg ctccgaaccg tcgatcgagc gcgctccgaa
     1921 accgatcacc gcgccgcgac ggttgcgaat ggggaacatc acgcggccgc gaaaacggtc
     1981 atagcgcttg ccttcgtccc cgacaatcac cagtcccgct tcggtcagac gcgcgtcctc
     2041 gtaggactcg aacgcttcac gcagggactg ccagccgtcg ggcgagaatc cgagggcgaa
     2101 gcgtgccgcg gtctggccgc tcagaccgcg gcgcttcagg tactcgatcg cggccggcga
     2161 ttccttcagc tttcgacgat agaagtcggc agcctgctgc agggcgtccg ccagcccgcg
     2221 cgtcttcgac gacggctcgc gcccgcccgt cgcttcgggc acctgcatac cgacctgctg
     2281 cgcgagttcg cgcacggcct cgacgaagcc cagcccgcgc gactccatca gaaagccgac
     2341 tgcagtgccg tgcacgccgc agccaaagca gtgatagaac tgcttgctcg gactgaccgt
     2401 gaaggagggg gtcttctcgc cgtgaaatgg gcacaggccg aggtagttct gcccggcctt
     2461 cttcagctgg acgtgacggc cgacgacgtc gacgatgtcg acgcgtgcga gcaggtcctg
     2521 aatgaaaccc tgcggaatca cggcaaaccg aatcagccgc cggcgagctt ctgcttgacg
     2581 agcgcggacg ccttgcccat gtcggctcgg ccggcgatgc ggggcttcag caacgccatc
     2641 accttgccca tcgcctgagg ccccccggcg ctggcggctg cgatagccgc gtcgacctcg
     2701 gccgcaagtt cgacgtcatt catctgctgc ggcaggtagg aggcgagcag gttcagctcc
     2761 gccgtttcct tggcgaccag gtcctgccgg cccgccttgt cgaactgggc gatcgagtcg
     2821 cgccgctgct tgatcagctt ctcgacgatc gtcatcaccg cggcatcgtc ggcgacgatg
     2881 cgctcgtcaa tctccttctg cttgaccgcg gccaacagca gccggatcgc ggacagacgc
     2941 tcggcctcgc gcgcgcgcat cgccgccttc atgtcctcgt tgattcgctc cttgagcgtc
     3001 attggcatct ccagaaagac aaagcgcgcc acgtgggcgc gctcacatca aggccggctc
     3061 tcgcggacga ctaatacagc ttcttcggca gcatctggct gcgcagacgc ttgaaatggc
     3121 gcttgatcgc cgccgacttc ttgcgcttgc gctcggcggt tggcttctcg tagaactcgc
     3181 gcgcgcgcag ttccgtgagc agtccgctct tttcgatcgt gcgcttgaag cgacgcaggg
     3241 caacttcgaa gggctcgttt tccttgaggc gaatcgtagg catgaagttc ctgaaagcaa
     3301 gggccggctg aaaaggtcgt gattgtatcg cggcgcggaa aaaattgaca agtcaaggcg
     3361 ttagcggcgg agccgcgaat gtgggcccgc taaactggtc gccgttccct ttcgagaatc
     3421 ccacgccgtg ctcgtcctcg gaatcgaaac ctcgtgcgac gaaaccggca ttgcgctgct
     3481 cgacgagaac agtggcctgc tcgcacacgc ggtgcactcg caggtcgaca tgcatcgcgc
     3541 ctacggaggc gtagtccccg aactggcgtc gcgcgaccac atccgccgcg tgctgcccct
     3601 gaccgaccgg gtgctggcgg acgcgaaacg gagtcgcgcc gacatcgatg cgatcgcggt
     3661 aacgatcggg ccgggtctga tcggcgcgct gctggtgggc gccagcattg cacacggctt
     3721 gggcgcggcg ctgcgccggc cggtgatcgg cgtgcatcac atggaaggcc acctgttgtc
     3781 gccgctgctg gcgaatcccg cgccggcttt cccgttcacc gcactgctgg tttcgggcgg
     3841 ccacacgcaa ctgctgcacg taggcagcgt gggcgactac cggctgcttg gcgaaaccgt
     3901 cgacgatgcc gcgggcgagg cgttcgacaa gaccgcgcag attctgggtc tcggctaccc
     3961 cggcgggccc gcggtttcgc ggctggcgga cttcggcacg ccgggcgcgg tgacgctgcc
     4021 tcggccgatg cttcacagcg gcgacctcgc cttcagcttc agcggcttga agaccgcggt
     4081 gctgaccgcg gctcgcagaa tcgattcgac gtgcgaacaa gcgcgcgcgg accttgcgcg
     4141 cggcttcgtg gacgcggtgg tcgacgtgct tgcgaccaaa tccatgcgcg cgctggaggc
     4201 atccggcgcc ggcacgctgg tggtggcagg cggcgtcggc gccaatgtgc agttgcgcga
     4261 gcggttgagc gatgaaacgc gccgcatcgg cgcgaccgtc tactttccgc cgcctgacct
     4321 ttgcaccgac aatggcgcaa tgatcgccgt cgcggggctc gctcgcctga agcgcggcgc
     4381 gttcgacgga agcggtgcct tcaccgtgca cccgcgctgg acgctttccg acgcacgcta
     4441 gtgccggcgc ggtggcacgg caaccaccgc cttctcgccg atcttgcgct ccttgccagc
     4501 cagcagctgg gcgatgttct tctgatgccg catgatcagc atcccactca tcacgatcga
     4561 agcgaccagc accggttcaa acccccatcc catcagttgc gccagcggc
//
LOCUS       DBNHBY010000487         5670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_640362, whole genome shotgun sequence.
ACCESSION   DBNHBY010000487 DBNHBY010000000
VERSION     DBNHBY010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_640362"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC16HB_12020"
     CDS             <1..546
                     /locus_tag="AC16HB_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5607562.1"
                     /translation="LLMAAMLIEIKSRMLLPVKKAETGEEVEDPRAELVRRLIEYERM
                     KLAAAQIDELPRLGRDFVRALVAIEQSLEKRFPEVHVIDLQQAWAEVLKRASLYAHHH
                     VARAELSVREHMSLVLKRLQSARFVEFGELFDAALLARGAPIVIVHFLALLELARENL
                     IEITQAAPYAPIYLRLAYTPS"
     gene            578..934
                     /locus_tag="AC16HB_12025"
     CDS             578..934
                     /locus_tag="AC16HB_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5607563.1"
                     /translation="MARITYPTVIRPRIAVRADFLIGPGKIDLLQAIEREGSISGAAR
                     ACGMSYKRAWSLLDELNRGLGRPVLVGITGGRGGGGATLTPSGRALVEHYLALERACA
                     DAAAAHLKILRRLFRA"
     gene            1013..1798
                     /gene="modA"
                     /locus_tag="AC16HB_12030"
     CDS             1013..1798
                     /gene="modA"
                     /locus_tag="AC16HB_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5607564.1"
                     /translation="MWIVRRRLLRSLGAMALTRTSMGVAAQSAPTIAAASDLKFALEE
                     IAAMFRRDTRQSVRLTFGSSGNFARQIEQGAPFALFMSADEEFVLRLAEHGLALDRGV
                     LYAIGRIVIMAPLGSPLVPDGQMKGLATALRDGKIKRFAIANPEHAPYGRRAGEALRH
                     AGLWDALRPHLVFGENIAQAAQFATSGSTQGGIIAYSIALAPQIAALGRFALIPSDWH
                     QPLRQRMALLRGADAAARAFYEYLQREPARAVMRKYGFVLPGE"
     gene            1812..2480
                     /gene="modB"
                     /locus_tag="AC16HB_12035"
     CDS             1812..2480
                     /gene="modB"
                     /locus_tag="AC16HB_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR5607565.1"
                     /translation="MDLQALRLSLELAALTMLTLLPIGAVVGRWLAVSHFRGRRVVEA
                     MLALPLLLPPTVLGFYLLLAMGAGSPLGALWTAMFGRPLAFSFQGLLLASVIANIPFA
                     IQPMQRAFESIGTDVREAAAVCGMTRWRTLWRIELPLAWPGILSGLVLTFAHTLGEFG
                     VVLMVGGSIPGETKTLSIAIYDRVQAFDERGAAIMAAALLAISLAAISTSYLSMHRFD
                     RRHV"
     gene            2473..3582
                     /locus_tag="AC16HB_12040"
     CDS             2473..3582
                     /locus_tag="AC16HB_12040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607566.1"
                     /translation="MSDAGLAVALRQTAPIPLDVSFTCAPGELLALVGPSGSGKTTIL
                     RMIAGLMRPLSGLIRCNDSVWLDGRAFARPRERRVGLVFQNYALFPHLTDLSNVMEAV
                     PANAGRGRAEIAADWLRRVHLAGFERRLPRELSGGQQQRVAVARAVAGRPSVLLLDEP
                     FSAIDRTTRESLYEELAELRHHLDMPAILVTHDLDEAALLADRMCLLSRGRTLQIGPP
                     GEVMMRPATVEAARLLGMKNVFEATVIAHQNDATVIDWRGRRLRVRAQPQYAPGARVA
                     WAIPSAGVLLPSETARGRALDTSIASIVAQVLALGERLRLVCRVEGTDAHLHTTVARH
                     VAWRYPLTPGAALALRLRGDHVHLMPPADSALHEH"
     gene            3572..4381
                     /locus_tag="AC16HB_12045"
     CDS             3572..4381
                     /locus_tag="AC16HB_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5607567.1"
                     /translation="MSTERGVPRAVASFAAGTAVGTMGGLIGLGGAEFRLPLLVMLFG
                     CATRDAIGLNVTFSLVTVAFALGFRLLGGGVAPLCEFAEALAALTIGSIAGAWMGVSI
                     GRRLATTALIRVAAVLLMALAVVMVSHAFDAAFGITAALSQPWRAPVAALAGVVIGNV
                     ASLLGVAGGELLIPALVLIYALDSKIAGTIALAISLPTLLISLARWRHALGDWQLWRS
                     NATLLIWMGAGSVAGAAIGTRLLGLVDARLLSLLLAALLMYSALHLARQER"
     gene            4465..>5670
                     /locus_tag="AC16HB_12050"
     CDS             4465..>5670
                     /locus_tag="AC16HB_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HIR5607568.1"
                     /translation="MIVSLCLTASVLSPASRADEPDPYLWLEDIAGERALAWVRERNV
                     EAEREFKADPRYEPLRARLLDIVTSRERIPYVTRMGEYVYNFWREGPNSRGVWRRTTP
                     AEYRTTAPNWEIVLDLDALAAREAENWVWKGAECLRPAKPDEPYRHCILELSRGGADA
                     VVLREFDLVDKRLVIGSGAFVLGEDKQDIRWKDADTVWVMSAFRPDEKTTSGYPRMAR
                     EWKRGRLASESPIVFEGKASDVGVEVRSERESGVRRDWFERSIAFWSSEYFVTVGGER
                     VKLDIPPDAQPIPFADWLLIRTRTPWTAGGTTYPGGALLAIRFDLFLRGSRSFFVLYE
                     PRPRASLADVATTKSAVLLTELDNVKSRLWELAFDGQNWNRARVPTPEFARVGIASAY
                     WASDEYQYTA"
BASE COUNT          797 a         1933 c         1981 g          959 t
ORIGIN      
        1 ctgctgatgg cggcgatgct gatcgagatc aagagccgga tgctgctgcc agtgaagaag
       61 gccgagaccg gcgaggaagt cgaggacccg cgcgccgaac tcgtgcgccg actgatcgag
      121 tacgagcgca tgaagctcgc cgccgcgcag atcgacgagc tgccacgcct cggccgcgac
      181 ttcgtgcgcg cgctggtggc gatcgagcag tcgctcgaga agcgcttccc cgaggtccac
      241 gtgatcgacc tgcagcaggc ctgggccgaa gtgctcaagc gcgccagcct gtacgcgcac
      301 catcacgtcg cgcgcgcgga gctttcggtg cgcgaacaca tgagcctggt gctgaagcgg
      361 ctgcagagcg cgcgcttcgt cgagttcggc gaactgttcg acgccgcgct gctcgcgcgc
      421 ggcgcgccga tcgtgatcgt gcatttcctc gcgctgctgg aactggcgcg cgagaacctg
      481 atcgagatca cgcaggcggc gccgtacgcg ccgatctacc tgcggctggc gtacacgccg
      541 agctgagcgt cggcgcggcg ctgggcactg ccctccgatg gcgcgcatca cctaccccac
      601 cgtcatccgg ccgcgcatcg cggtgcgggc cgactttctc atcggtcccg gcaagatcga
      661 cctgctgcag gcgatcgagc gcgaggggtc gatcagcggc gccgcgcgcg catgcggcat
      721 gagctacaag cgcgcctggt cgttgctcga cgaattgaac cggggcctgg gccgcccggt
      781 gctcgtcggc atcaccggcg gtcgcggcgg cggcggggct acgctgaccc ccagcgggcg
      841 ggcgctggtc gagcactacc ttgcgctcga acgcgcctgc gcggacgcgg cagccgcgca
      901 tctgaagatc ctgcgacgcc tgtttcgcgc ctagatgcgc ctggatgggc ccgccggccg
      961 acccggcgtc gttatgtcag aatcaccata acgccggtcg gggagaagtt gcgtgtggat
     1021 tgtgcgtcgc cgcctgctgc gcagccttgg tgcaatggcc ctgacgcgca cttccatggg
     1081 cgtggcggcg cagtcggcac cgacgatcgc cgcggcgtcg gatctgaagt tcgcgctcga
     1141 ggagatcgcc gccatgttcc gccgcgacac tcggcagtcg gtgcgattga cgtttggctc
     1201 ctcgggcaat ttcgcgcggc agatcgaaca gggcgcgccg ttcgcgctct tcatgtcggc
     1261 cgacgaggaa ttcgtcctgc gcctcgccga gcacgggctg gcactcgatc gcggcgtgct
     1321 ttacgcgatc ggtcggatcg tgatcatggc accgctcggg tcgccgctgg tacctgacgg
     1381 gcagatgaag gggctcgcga cggcgctgcg cgacgggaag atcaagcgct tcgcgatcgc
     1441 caaccccgaa cacgcgccgt acggccggcg cgccggggag gcgctgcgcc acgccggact
     1501 gtgggatgcg ctgcgcccgc acctggtgtt cggcgagaac atcgcgcagg ccgcgcagtt
     1561 cgcgacctcg ggcagcaccc agggcggcat catcgcgtac tcgattgcgc tcgcgccgca
     1621 gatcgccgcg ctcggccgct ttgcgctgat cccgtcggac tggcatcaac cgctgcgaca
     1681 gcgcatggcg cttctccgag gcgcggatgc ggccgcgcgc gccttctacg aatacctgca
     1741 acgcgagcca gcgcgcgcgg tcatgcgcaa gtacggcttc gtgctgccgg gcgagtgaga
     1801 cgcgacgacg gatggatctc caggcactgc gactgtccct cgaactggcg gcgctgacga
     1861 tgctgacgct gctgcccatc ggcgccgtcg tcggccgctg gctggcggtg agccacttcc
     1921 gcgggcggcg cgtggtcgaa gccatgctgg cactgccgct gctgctgccg ccgacggtgc
     1981 tcggcttcta tcttctgctg gcaatgggcg cgggatcgcc gctcggtgca ctgtggaccg
     2041 cgatgttcgg ccgcccgttg gcgttcagct tccagggcct gctgctggcg tccgtgatcg
     2101 ccaacatccc cttcgcgata cagccgatgc agcgcgcgtt cgaatcgatc ggcaccgatg
     2161 tacgcgaggc agccgccgtg tgcggcatga cgcgctggcg caccctgtgg cgcatcgaac
     2221 tgccgctggc ctggcccggc atcctttccg gtctggtgct gacgtttgcg cacacgctcg
     2281 gcgagtttgg cgtggtgctg atggtcggcg gcagcattcc gggggagacg aagacgctat
     2341 cgatcgccat ctacgatcgc gtgcaggcct tcgacgagcg cggcgccgcg atcatggccg
     2401 ccgcactgtt ggcgatctca ctggcggcca tctcgacttc gtatctgagc atgcaccgct
     2461 tcgatcggcg ccatgtctga cgctggcctc gcggtcgctt tgcggcagac cgcgccgatc
     2521 ccgctcgacg tgtcgttcac ctgcgcgccg ggcgaactgc tggcactggt cggcccttcg
     2581 ggaagcggca agacaacgat cctgcgcatg atcgccggcc tgatgcggcc gctgtccggc
     2641 ctcatccggt gcaacgattc ggtctggctc gacggccgcg cgttcgcgcg gccgcgcgag
     2701 cgtcgcgtgg gtctcgtatt ccagaactac gcattgttcc cgcacctgac cgacctttcc
     2761 aatgtgatgg aggcggtgcc cgcgaacgcg ggtcgaggcc gcgcggaaat cgccgccgat
     2821 tggctgcgtc gcgtacacct cgccggcttc gagcgtcggc tgccgcgcga actttccggc
     2881 ggccagcagc agcgcgttgc ggtggcccgc gcggtggccg gacggccgag cgtgctcttg
     2941 ctcgacgagc cgttttcggc catcgaccgc acgactcgcg agagcctgta cgaggagctc
     3001 gccgaactgc gccaccatct cgatatgccg gcgatcctgg tcacgcacga cctggacgaa
     3061 gcggctctgc tcgccgaccg catgtgcttg ctttcgcgag ggcggaccct gcagatcggt
     3121 ccgccgggcg aagtgatgat gcggccggcg acggtcgagg cggcacgcct gctgggcatg
     3181 aagaacgtgt tcgaggcaac cgtcatcgcg catcagaacg atgcgaccgt gatcgactgg
     3241 cgcgggcggc ggctgcgcgt gcgcgcgcaa ccgcaatacg caccgggcgc acgagtcgcg
     3301 tgggcaatcc cgtcggcggg cgtactcctg ccgtccgaga ccgcccgcgg gcgtgcgctg
     3361 gatacctcga ttgcatccat cgtggcgcaa gttctggcgc tgggggaaag gctgcgtctg
     3421 gtctgccgcg tcgaaggcac cgacgcccat ctgcacacta ccgtcgcgcg gcacgtcgcg
     3481 tggcgctatc cgctcacgcc gggagccgcg cttgctttgc gactgcgcgg cgatcatgtt
     3541 cacctgatgc cgccggccga ttccgcgctt catgagcact gaacgcggcg tgccccgcgc
     3601 ggttgccagc ttcgccgccg gcactgccgt cggcacgatg ggcggactga tcgggctcgg
     3661 cggcgccgag ttccgcttgc cgctgctggt catgctgttc ggctgcgcga cgcgcgacgc
     3721 gatcggactg aacgtgacgt tcagcctcgt tacggttgcc ttcgcactgg gctttcggct
     3781 gctcggcgga ggcgttgcgc cgctgtgcga atttgccgaa gcgctcgccg cgctcaccat
     3841 tggatcgatc gccggcgcgt ggatgggcgt ctcgatcggc cgacgcctgg ccaccacggc
     3901 gttgatccgc gtggccgcgg tgctgctcat ggcgctcgcc gtggtgatgg tttcgcatgc
     3961 gttcgacgct gccttcggca tcacggcggc gctatcgcag ccgtggcgag cgcctgtcgc
     4021 cgccctcgcc ggcgtcgtga tcggcaacgt cgccagcctg ctcggcgtgg cgggcggtga
     4081 actgctgatt ccggccctcg tgctgatcta cgcgctcgac tcgaagatcg ccggcaccat
     4141 cgcgctggcg atcagcctgc cgacgctgct catatcgctt gcgcgttggc ggcatgcgct
     4201 tggcgactgg caactgtggc ggtcgaacgc gacgctgctc atctggatgg gcgcaggcag
     4261 cgtcgccggc gcggccatcg gcacgcgatt gctcgggctg gtcgacgcgc gactgctgag
     4321 cctgctgctc gccgcgctgc tcatgtattc cgcgctgcat ctggcgcgac aggaacgctg
     4381 atgcgcgcat gccgcaaccc gcagcgcggg ggctatcctc gcccggtcgt cacgtcctgc
     4441 ctcacggccg ccgccatgcg ccgaatgatc gtttcgctct gcctgaccgc gtcggtgctg
     4501 tcgcctgcca gccgcgccga cgaaccggat ccgtatctgt ggctcgagga cattgccggc
     4561 gaacgcgcgc tggcatgggt gcgcgaacgc aacgtcgaag ccgagcgcga gttcaaggcc
     4621 gacccgcgct acgagccgct gcgcgcgcgc ctcctcgata tcgtcacgtc gcgcgagcgc
     4681 atcccgtacg tgacgcggat gggcgagtac gtctacaact tctggcgcga ggggccgaac
     4741 tcgcgcggcg tgtggcgccg gaccacgccg gccgagtacc gcacgacagc gccgaactgg
     4801 gagatcgtgc tggacctcga cgcgctcgcc gcacgcgaag cggagaactg ggtatggaag
     4861 ggtgccgagt gcctgcgtcc cgcaaagccc gacgaaccgt accgccactg cattcttgaa
     4921 ctgtcgcgcg gcggcgccga tgcggtcgtg ctgcgcgagt tcgatctcgt cgacaaacgg
     4981 ctcgtgatcg ggtccggggc attcgtgctc ggcgaggaca agcaggacat ccgctggaag
     5041 gacgccgaca cggtgtgggt gatgtcggca ttccgacccg acgagaagac gacttccggc
     5101 tatccgcgca tggcgcgcga atggaagcgc ggtcggctcg cgtccgaatc gcccatcgtg
     5161 ttcgagggca aggccagcga tgtcggcgtc gaagtgcggt ccgagcgcga gtcgggcgtg
     5221 cgccgggact ggttcgagcg ttcgatcgcg ttctggagca gcgaatattt cgtgacggtc
     5281 ggcggcgagc gcgtgaagct cgacatcccg cccgatgcgc agccgatacc ctttgcggac
     5341 tggctgctga tccgcactcg cacgccgtgg accgcgggcg gcaccacgta tccgggcggc
     5401 gcgctgctgg cgatccgctt cgacctcttc ctgcgcggct cgcgcagctt cttcgtgttg
     5461 tacgagccgc ggccgcgcgc cagcctggcc gacgtcgcga ccacgaagtc cgccgtgctg
     5521 ctgaccgaac tcgacaacgt gaagagccgg ctgtgggagc tcgcgttcga cggtcagaac
     5581 tggaatcgcg cgcgcgtgcc gacgcccgaa ttcgcgcgcg tcggcatcgc gagcgcgtac
     5641 tgggccagcg acgaatacca gtacaccgcg
//
LOCUS       DBNHBY010000488         2981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_911560, whole genome shotgun sequence.
ACCESSION   DBNHBY010000488 DBNHBY010000000
VERSION     DBNHBY010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_911560"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1730)
                     /locus_tag="AC16HB_12055"
     CDS             complement(126..1730)
                     /locus_tag="AC16HB_12055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607569.1"
                     /translation="MNPFDADKRALYAREIDDDKRTSLSVLLGLVRPGTEVLDVGIGG
                     GALGRRLTTERGCRVDGVTLSEVEAAVAAAHYRRIEIADLDRTSLAGLFAGQRYDAII
                     CADVLEHIARPTQVLDACRDLLADTGVLLLSIPNVAYTGLIGELLAGEFRYRIEGLLD
                     RTHVRFFTRRSLLRMLHDSGWDVDLVEPIKLDLPESEFNVEFGRLPPAIQRYLLTLPD
                     GMTYQFIVRARPHVAGARTGAFDITEPPATEPAPLDAPHFSSLLYLNDGSGYSEDRKV
                     AAQGRIGEARQCIRFAVPAQAKPLIGLRIDPADRPGFLHLFGMRMLAADGDTVWQWDG
                     RNETLALGEHKQVIVRTPWFGDHGAVVLLTGEDPYFELPIDRTALARCQTGAVLEVDM
                     GWPMSADYALFVGELSALQASLAQARADAQRSAQLRESLERDLADRRAEVQALASEKV
                     RARQLEQKLDAETASVQRLQRESAQLAAERERFRTQADQLGITVQQAQRDIADLRRRV
                     EQQRAQGNAVVRERDALRARLAQIQD"
     gene            1832..2719
                     /gene="rfbD"
                     /locus_tag="AC16HB_12060"
     CDS             1832..2719
                     /gene="rfbD"
                     /locus_tag="AC16HB_12060"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR5607570.1"
                     /translation="MRILITGRDGQVGWECQRSLHTLGTVVALGRDDCDLARPASIQA
                     AIAAADPDLIVNAAAYTAVDRAESEPDLAHAVNAEAPALIAAEARRRGSALIHLSTDY
                     VFDGGQSEPYVEDDVPNPMSVYGRTKLLGEHGIMNAGAAALVLRTSWVYAERGHNFVR
                     TILRLAREREELRVVGDQWGAPTWARSIAAGIAAIVARAGTDRESITGSFARRGGLFH
                     MAASGKTNWHAFAEHIVARVPDPTRKLERIVPISTADYPTAARRPFNSLLSCARLARE
                     WNVAIPDWDLAFGWAFSAG"
     gene            complement(2745..>2981)
                     /locus_tag="AC16HB_12065"
     CDS             complement(2745..>2981)
                     /locus_tag="AC16HB_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607571.1"
                     /translation="YYFEQLAPDLRAHAIAYAPEEIPVMRESLEFRNKRFVWYLQGFT
                     RTGRNAVLDQLAADYVPLRHERFAAVEATLFEKR"
BASE COUNT          489 a         1021 c         1001 g          470 t
ORIGIN      
        1 gcggcggatg gcggcagctc gcgcaaccgg gacacgccgg ccaacgcgct gcgcggcgcc
       61 atgccgcgca gctcgatccc gctgcgcaac gacggactga aaagtgccgc caggccgtac
      121 agccgctaat cctggatctg cgccagccgt gcccgcaggg cgtcgcgctc ccggacgacg
      181 gcgttgccct gcgcgcgctg ctgctcgacc cgccgcctca gatcggcgat gtcgcgctgc
      241 gcctgttgca ccgtgatgcc gagctgatcc gcctgggtcc gaaacctctc gcgctcagcg
      301 gccagttgcg cggattcccg ctgcaagcgc tgcaccgatg ccgtttcggc gtcgagcttc
      361 tgctcgagct gccgcgcgcg taccttctcg ctcgccagcg cctgcacctc tgcgcgccgg
      421 tcggcgaggt cccgctcgag agactcccgc agctgggccg agcgctgtgc gtcagcgcgt
      481 gcctgcgcca ggctcgcctg cagcgcactg agttcaccga cgaacagggc atagtcagcc
      541 gacatcggcc agcccatgtc gacctcgagc accgcgccgg tctgacaacg cgccaatgcc
      601 gtgcgatcga tcggcaattc gaaatacgga tcctctcccg tcaatagcac gaccgcgccg
      661 tggtccccga accacggcgt ccggacgatc acctgcttat gctcgccgag cgcaagcgtc
      721 tcgttcctgc cgtcccactg ccagaccgtg tcgccatcgg ccgcgagcat ccgcatgccg
      781 aaaaggtgca ggaaccccgg ccggtctgcc ggatcgatgc gcaggccgat gagcggcttc
      841 gcctgtgcag gcaccgcgaa gcggatgcac tggcgggcct ccccgatccg cccctgcgcg
      901 gcgactttcc tgtcttccga gtagccgctg ccgtcgttca gatagagcag cgacgagaaa
      961 tgcggcgcgt ccagtggggc tggttccgtt gccggcggtt ccgttatgtc gaaggcgcct
     1021 gtgcgcgcgc cggcaacatg cgggcgggca cggacgatga actggtaggt catgccgtcg
     1081 ggcaaggtca gcaggtaacg ctgaatggcc ggcggcaggc ggccgaattc gacgttgaac
     1141 tcggactccg gcagatccag cttgatcggc tcgaccagat cgacgtccca cccgctatcg
     1201 tgcagcatcc gcagcagcga acgccgcgtg aagaagcgga catgcgtgcg gtcgagcaga
     1261 ccctcgatgc gataccggaa ttcgcccgcg agcagttcgc cgatcagccc ggtgtaggca
     1321 acattcggga tcgacagcag cagcacgcct gtgtcggcga gcagatcgcg gcaggcatcg
     1381 agaacctggg tcggccgcgc gatgtgctcc agcacgtcgg cgcagatgat tgcgtcatag
     1441 cgctgccccg cgaagagtcc ggccagactg gtacggtcca gatcggcgat ttcgatgcgc
     1501 cgatagtgcg cggctgcgac agcggcctcg acctcgctca acgtgacgcc atcgacccgg
     1561 cagccgcgct ccgtggtcag gcgccgcccg agcgcgccgc caccgatgcc gacatcgagc
     1621 acctcggtgc cgggccgcac cagtccgagc aacaccgaca gcgacgtgcg cttgtcgtcg
     1681 tcgatctcgc gtgcgtacag cgccctcttg tcggcgtcga acggattcat caagaagcgg
     1741 cagggaagat gggaaagcat gcattttacg tgctagggtg aggcgccatt cgggcattgc
     1801 acggcaactg cagcaccttc gagtgcaagc gatgcgaatc ctgattaccg gtcgtgatgg
     1861 ccaggtcggc tgggaatgcc agcgcagcct gcacacgctc ggaacggtcg tcgcgctcgg
     1921 gcgggatgac tgcgatctcg cgcgcccggc gtcgattcag gcggccatcg cggcggcgga
     1981 tccggacttg atcgtcaatg ccgcggcgta taccgcagtc gaccgcgccg aatcggaacc
     2041 ggatctggcg cacgcggtga atgccgaggc gcccgcgctg atcgcggccg aggcgcgccg
     2101 gcgcggaagc gcgctgatcc acctgtcgac cgactacgtg ttcgacggcg ggcagtccga
     2161 accctatgtc gaagacgacg tgccgaaccc gatgtcggtc tacggccgca cgaagctgct
     2221 cggcgagcac ggcatcatga acgccggagc cgccgccctc gttctgcgca cgagctgggt
     2281 ctatgccgag cgcggacaca atttcgtgcg cacgatcctg cgtctcgcgc gcgagcgcga
     2341 ggaactgcgc gtcgtgggcg atcaatgggg ggcgccgacg tgggcgcgct cgatcgccgc
     2401 cggcatcgcc gccatcgtcg cgcgtgccgg caccgatcgg gagtcgatca ccggctcgtt
     2461 cgcccggcgc ggcggcctgt tccacatggc cgcgagcgga aagaccaact ggcatgcatt
     2521 cgccgagcac atcgtcgcgc gggtgccgga cccgacgcgc aagctcgagc gcatcgttcc
     2581 gatttcgacc gcggactacc cgacggcggc gcgccggccg ttcaactcgc tgctttcatg
     2641 cgcgcgcctc gcacgggagt ggaacgtcgc gatcccggac tgggatctgg cattcggctg
     2701 ggccttctcg gccggatgat gggcgagcag cctttcgcct cgcgctagcg cttctcgaaa
     2761 agcgtcgctt cgaccgcggc gaagcgctcg tgccggagcg ggacgtagtc ggcggcaagc
     2821 tgatcgagca cggcgttgcg gccggtacgg gtgaacccct gcagatacca gacgaagcgt
     2881 ttgttgcgga actccaggga ttcgcgcatc accggaatct cctccggtgc atacgcgatg
     2941 gcatgcgcgc ggagatccgg tgcaagttgc tcgaagtagt a
//
LOCUS       DBNHBY010000489         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_235422, whole genome shotgun sequence.
ACCESSION   DBNHBY010000489 DBNHBY010000000
VERSION     DBNHBY010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_235422"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..138)
                     /locus_tag="AC16HB_12070"
     tRNA            complement(65..138)
                     /locus_tag="AC16HB_12070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Gly,seq:ccc)
     gene            complement(158..1441)
                     /locus_tag="AC16HB_12075"
     CDS             complement(158..1441)
                     /locus_tag="AC16HB_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR5607572.1"
                     /translation="MKRLPIPLRDKVRATLHALGPGLVTGAADDDPSGIATYSQAGAQ
                     FGFGLLWTTLLTTPFMIAIQLAAARVGRVTGRGLAGNLRAHYPRPLVLALVALLVAAN
                     TINITADIAAMGEALQLVIGGPNHGHALVFGVVLAWVQVRLPYYRLAEIFKWLCLSLL
                     AYAAVLLYVNVDWFAAARGFVWPRIDFTFDALMMVVALLGTTISPYLFFWQASHEVEE
                     LRRKGGVPLAQAPERAPGALLRIRFDTVSGMVFSNLIALAIMLAAATTLHAHGVTDIQ
                     TSAQAAEALRPIAGDRAFLLFALGIVSTGLLAVPVLAGSAAYAVSEAFDWGGGLDHTW
                     QEARGFYTIVIGCTIAGTLLDFTSIDPVKALFYSAVINGVISVPIMAMLVLLASNREA
                     LGEFAINGALRWFGWLATGLMAVAVALMLVAAVRN"
BASE COUNT          293 a          564 c          555 g          241 t
ORIGIN      
        1 tgggcggcgc agcggtcctg ctctgggtgg cgctcagccg atatgtgccg ttgatgcagg
       61 agactggagc gggtgatggg aatcgaaccc acgtctgagg cttgggaagc cgccgtccta
      121 ccattgaacg acacccgctt cggggccgga atttacccta gttcctcact gccgcgacca
      181 gcatcagcgc gactgcgacc gccatcagcc cggtcgccag ccagccgaac cagcgcagcg
      241 caccgttgat cgcgaactcg ccgagcgcct cgcggttgct cgccagcagc accagcatcg
      301 ccatgatcgg caccgagatc acgccgttga tcactgcgct gtagaacagt gccttgaccg
      361 gatcgatcga cgtgaaatcg agcagcgtac cggcgatcgt gcagccgatc acgatcgtgt
      421 agaagccgcg cgcctcctgc caggtgtggt cgaggccgcc gccccagtcg aacgcttccg
      481 acaccgcgta cgcagcggag cctgcgagca ctggcacggc cagcaatccg gtggaaacga
      541 tgccgagggc gaacagcagg aacgcgcggt caccggcgat cggccgcagc gcctcggcgg
      601 cttgcgccga ggtctgaatg tcggtcacgc cgtgggcgtg cagcgtggtc gcggcggcga
      661 gcatgatcgc gagcgcgatc aggttggaga acaccatgcc ggacacggtg tcgaagcgga
      721 tccgcagcag tgcacccggc gcgcgctccg gcgcctgcgc gagcggcacg ccgcccttgc
      781 gccgcagttc ctcgacctcg tgcgacgcct gccagaagaa caggtacggg ctgatcgtcg
      841 tgccaagcag cgcgaccacc atcatcagcg catcgaacgt gaagtcgatg cgcggccaga
      901 cgaaaccgcg cgcggcagcg aaccagtcga cgttgacgta cagcagcacc gccgcgtacg
      961 cgagcagcga caggcacagc cacttgaaga tctccgccag gcggtagtag ggcagccgca
     1021 cctgcaccca tgcgagcacg acgccaaaca cgagcgcatg accgtggttc ggcccgccga
     1081 tcacgagctg cagcgcctcg cccatcgcgg cgatgtcggc ggtgatgttg atcgtgttgg
     1141 cggcgacgag cagcgcgacc agcgcgagca ccagcgggcg cggatagtgc gcgcgcaggt
     1201 tgcccgccag cccgcgcccg gtcacgcgcc cgacgcgcgc ggcggcaagc tggatcgcga
     1261 tcatgaacgg cgtcgtcagc agcgtcgtcc acagcaggcc gaagccgaac tgcgcgcccg
     1321 cctgcgaata ggtcgcgatg ccgctcgggt cgtcgtcggc cgcgccggtg accaaccccg
     1381 gccccagcgc gtgcagcgtg gcgcgcacct tgtcgcgcaa cgggatcggg agtcgtttca
     1441 ctttcttctt cgggcgccaa cagggcccgc gtcgatatac acgaaaatcg agcttcgttc
     1501 gtgatcgaat gtgtcgcccg acaaggagca tggcttggat ctggccgcgc tgatatcgga
     1561 ccatggctac tgggtgctcg ccctcggcac gctgctcgag ggcgagaccg tgctcgcgct
     1621 ggcgggcttc gctgcgcacc agggctatct ttc
//
LOCUS       DBNHBY010000490         6155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_99146, whole genome shotgun sequence.
ACCESSION   DBNHBY010000490 DBNHBY010000000
VERSION     DBNHBY010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_99146"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..581
                     /locus_tag="AC16HB_12080"
     CDS             117..581
                     /locus_tag="AC16HB_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5607573.1"
                     /translation="MNFEIPLPLRQEHEELHERLRQATQAGGEVGAAAKALAQLMHPH
                     FVKEDEYALPPLGLLQSIAQGEVTPAMAGVLALTDRLAAELPAMLAEHQAIVAALARL
                     QDAATRAGRRDIVEFAAALALHAQTEEQVMYPAALLVGRFVRQRLGEAAAIH"
     gene            602..1234
                     /locus_tag="AC16HB_12085"
     CDS             602..1234
                     /locus_tag="AC16HB_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004536980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607574.1"
                     /translation="MPMRTPDFFDAVPAIAVHDPLAALLGAAEDGRIEYRYADAVKLA
                     GHSCPTVASAWLMTARALHALYADALPERGNIRVEVRGAQEEGVEGVIALVAGLVTGA
                     AGPGGFKGIAGQHARRDLLQFGAPLHGRLRFTRLDTQRAVEVDYHAERVPMPPEMRPL
                     LAAATAPNASIDARRAFGDCWQAWVRTILIDHRDDPRLVEVHEAHVAAPA"
     gene            1245..1943
                     /locus_tag="AC16HB_12090"
     CDS             1245..1943
                     /locus_tag="AC16HB_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5607575.1"
                     /translation="MARREVAAHELLANVALFKQLDAAARARIAAHVNKRRLRRGEFA
                     FHRGEEPSGFFVVVYGEIALTAPGARGPRLTGLVGAGRSFGEPMLFLAKPYIVDARAH
                     TDALLLFVPKDAVFAEIERNPAFAHRMIAGLAARIELLVRELDTQARGSARERLVDYL
                     LRAAGVRQGVAVIELPTTKAALATQLGLTAEHLSRLLRDLAERDLIRVDGRRIEIFDA
                     AALAHVRRRGSGSG"
     gene            complement(1915..2739)
                     /gene="pyrF"
                     /locus_tag="AC16HB_12095"
     CDS             complement(1915..2739)
                     /gene="pyrF"
                     /locus_tag="AC16HB_12095"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR5607576.1"
                     /translation="MKFTQKLAARWQRADTLLCVGLDPDTTRLPAHLRDRDDAIYEFC
                     RAIADATAELVCAFKPQIAYFAAARAEDQLESLIAHLRGRHPDVPIILDAKRGDIGAT
                     SEQYAREVFERYGADAVTLQPYQGLDAIAPYLAYPGRGAFVLCRTSNPGGDALQMIDV
                     GGERLFERVARLIRDEWNTSGQLGLVVGATYPAELARVRAIVGEMPLLVPGIGAQGGD
                     VQAAVQAGQSRGGTGMVINSSRAILYASHGENFAEAARAAALAARDQINRFRNRGV"
     gene            2805..3686
                     /locus_tag="AC16HB_12100"
     CDS             2805..3686
                     /locus_tag="AC16HB_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607577.1"
                     /translation="MAALSRRDLVLLALLTLAWGVNWPVMKLGVTSFPPLLFRVIVLS
                     GGVAVLWLWIRGRRIPLAVPHGKRLTVLWLAVPNVIIWQILSVLALKLLPSGRAAILG
                     YTMPVWAVVVSVAFFGDRPLPRHWLGIAAAFVGTLLLLATEFTALAGRPLGTLLMLIS
                     AATWGWGTVLMRRHMLDLPTVTLGFWMLAAAVPVAVVTTLAFETSLWRLPTPTEWAAM
                     LYNILVAIAFCHIVWFHLARLLPPAASGLSVMLIPVVGVFSSMAMLGERPHWQDYTAL
                     ALILAALATVLLPQRPK"
     gene            complement(3852..>6155)
                     /locus_tag="AC16HB_12105"
     CDS             complement(3852..>6155)
                     /locus_tag="AC16HB_12105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5607578.1"
                     /translation="AVHDELDADLLPVFLTEGGDLLPAIGAQLRALAARPGDRDVARE
                     LMRQLHTVKGSARMAGAMRLGEVVHDMETRIEASMHLADVPPALVEDLHGAYDRAMSL
                     FEGLQSGTAPTPAPAPIEAAVAPSKAAEAPVATEEVTATPAAPVTAQTANYIRVRADV
                     LDKLVDHAGEVSIARAKLENEITTLRGAIVDLTENIQRLRSQLREVEIQADAQIHARS
                     DQLSRESAAFDPLEYDRYTRLQELTRMLRETVEDVAMVQGNMLKGLQLADTDLTAQGR
                     LTRELQQQLMRVRLVTFASISERLYRVARQAAKELDKRVNLDIRGANTEIDRGVLEKM
                     GGPFEHLVRNAIAHGLEAPAQRRAAGKPETGELKIDMRQEANEIIVVIADDGPGLNLE
                     RIRARAIERQLIAPDQPVSERELIELIFAPGFSTADEVTELAGRGVGMDVVRSELSSF
                     GGRIAVGTEAGRGTRFTLYLPLTLAVMQAVLARVGQRRFALPSGMVEQVLRFRPAELA
                     RSIGDGMIEIPGVGPIVLRPLAQLLGEEITLAHVKQIPVIVLKSGADRLAIAVDDVSN
                     NQEIVVKNVGASVSRLAGVLGATILGNGEIVLIINPVQLIMRAPEPPTLADAHFMLPA
                     DAGGAEDAGEIAAAAPPQVMVVDDSLTVRRVTQRLLERHGYEVLQAKDGVDALRQLQD
                     NRPDLMLVDIEMPRMDGFDLTRNVRGNAATRDIPIIMITSRTAEKHRRLAFDIGVNEY
                     LGKPYQEDELIALIQRYLAERKQQVTA"
BASE COUNT          875 a         2126 c         2127 g         1027 t
ORIGIN      
        1 ggtccgtccg gcgcagggcg caggcaaggt cgtcaccgcg ctgaacgaat acggccgcta
       61 ctttcaccac cccggctgga agccgatcaa ggccgggcac tgagcggagg gatgccatga
      121 acttcgagat tccgttgccg ctgcggcagg agcacgagga actgcacgag cgcctgcgcc
      181 aagcgacgca ggccggcggc gaagtgggcg ccgccgcaaa agcgctggcg cagctgatgc
      241 atccacactt tgtcaaggaa gacgaatacg cgctgccgcc gctcggcctg ctgcagtcga
      301 tcgcgcaggg cgaggtcacg cccgcgatgg cgggcgtgct ggcgctgacc gaccgcttgg
      361 ccgccgaact gccggcaatg ctcgccgagc accaggcgat cgtcgcggcg ctggcacggc
      421 tgcaggacgc ggcgacgcgc gccggccgcc gcgacatcgt cgagttcgcc gcggcgctcg
      481 cgttgcacgc gcagaccgag gagcaggtga tgtatccggc tgcgctgctg gtgggtcggt
      541 tcgtccgcca gcggctcggg gaagcggcag cgatacattg aaggagaaga tcgatatgac
      601 gatgccaatg cgcaccccgg acttcttcga cgctgtgccc gcgatcgcag tccatgatcc
      661 gctggccgcg ttgctcggcg ccgccgagga cggccgtatc gagtaccgct acgccgatgc
      721 cgtgaaactc gccgggcact cgtgcccgac ggtggccagc gcctggctga tgaccgcgcg
      781 ggcgctgcac gccctgtatg ccgacgcgct gcccgagcgc ggcaacatcc gggtcgaggt
      841 gcgcggcgcg caggaggagg gtgtcgaagg cgtaatcgcg ctcgtcgcgg gtctcgtcac
      901 cggcgccgcg gggcccggcg gcttcaaggg catcgcgggc cagcacgcac ggcgcgacct
      961 gctgcaattc ggcgcaccgc tccacggccg cctgcgtttc acgcggctcg acacgcagcg
     1021 cgcggtggag gtcgactatc acgctgagcg cgtgccgatg ccgccggaga tgaggccgct
     1081 gctagcagcc gcgaccgcgc ccaatgcgtc catcgatgcg cgccgcgcgt tcggcgactg
     1141 ctggcaggcg tgggtgcgga cgatcctgat cgaccaccgc gacgatccgc ggctcgtcga
     1201 agtgcacgaa gcgcacgtgg ccgcgccggc ctgaaggcgc gaaaatggct cggcgtgaag
     1261 tcgccgcgca cgaactgctg gcgaacgtgg ccctgttcaa gcagctcgac gccgcggcgc
     1321 gcgcacgcat cgccgcgcac gtgaacaagc ggcggctgcg acgcggcgag tttgcgtttc
     1381 atcgcggcga ggagccgagc gggttcttcg tggtggtcta tggcgaaatc gcgctgaccg
     1441 cgcccggtgc ccgcgggccg cggctgaccg gtctcgtcgg cgccgggcgc agcttcggcg
     1501 agccgatgct gttcctcgcc aagccgtaca tcgtcgacgc gcgcgcccat accgacgcac
     1561 tcttgctctt cgttccgaag gacgcggtgt tcgccgagat cgagcgcaac ccggcgttcg
     1621 cgcaccggat gatcgcggga ctggccgcgc gcatcgagct gctggtgcgc gaactcgaca
     1681 cgcaggcgcg cggctcggcg cgcgagcggc tggtcgacta tctgctgcgc gccgccggtg
     1741 tgcggcaagg cgtggcagtc atcgaactgc cgaccaccaa ggcggcgctt gccacgcagc
     1801 tcggtctcac cgccgagcac ctgtcgcggt tgctgcgcga tctcgccgaa cgcgacctga
     1861 tccgagtcga cggccgccgc atcgagatct tcgatgcggc tgcgctggcg cacgtcagac
     1921 gccgcggttc cggaagcggt tgatctggtc gcgcgctgca agcgcggccg cccgcgccgc
     1981 ctccgcgaag ttctcgccgt ggctcgcata caggattgcg cgcgacgagt tgatcaccat
     2041 gccagtgccg ccgcgcgact gtcccgcctg cacggcggcc tgcacgtcgc cgccctgcgc
     2101 gccgatgcct ggaaccagca gcggcatttc gccgacgatc gcgcgcacgc gcgcgagctc
     2161 ggccggatag gtcgcgccga ccacgagacc cagctgtccg ctggtgttcc actcgtcgcg
     2221 gatcaggcgc gcgacgcgct cgaacagccg ttcgccgccg acatcgatca tctgcagcgc
     2281 atcgccgccc gggttgctgg tgcggcacag gacgaaagcg ccgcgcccgg ggtaggcaag
     2341 gtaaggagcg atggcatcga gcccctggta cggctgcagt gtcaccgcat cggcgccgta
     2401 gcgctcgaac acctcgcgcg catactgctc gctggtcgcg ccgatgtcgc cccgcttggc
     2461 gtcgaggatg atcggcacgt cgggatgcct tccgcgcagg tgggcgatca ggctttctag
     2521 ctggtcttcg gcgcgcgctg cggcaaagta ggcgatctgc ggcttgaacg cgcacacgag
     2581 ttcggcggtc gcatcggcga tcgcacggca gaactcgtag atcgcatcat cgcgatcgcg
     2641 caggtgcgcg ggcagccgtg tcgtgtccgg atcaaggccc acgcacagca gcgtatcggc
     2701 acgttgccag cgcgccgcga gcttctgagt gaatttcaag tcgtacctcc tgcgcctgcc
     2761 tcggattcta gaggcgtgta acctgcgcgc cgctgcgatt cgatatggct gcgctttccc
     2821 gtcgcgatct cgtgctgctg gcgttgctca cgctcgcgtg gggcgtgaac tggccggtga
     2881 tgaagctcgg cgtcacgtcg tttccgccgc tcctgttccg cgtgatcgtg ctttccggcg
     2941 gcgtggcggt gttgtggctg tggatccgcg ggcgccggat cccgcttgcc gtgccgcacg
     3001 gcaagcgcct caccgtgctg tggctcgcgg tgcccaacgt gatcatctgg cagatcctgt
     3061 ccgtgctggc gctgaagctc ttgccgtcgg ggcgcgcggc catcctcggc tacacgatgc
     3121 cggtgtgggc ggtggtcgtc agcgtggcat tcttcggcga ccgtccgctg ccgcgccact
     3181 ggctcggcat cgcggccgcg ttcgtcggca cgctgctcct gctcgcgacc gagttcaccg
     3241 cgcttgccgg ccgcccgctc ggaacgctcc tgatgctgat ctccgccgcg acctggggct
     3301 ggggcaccgt gctgatgcgc cgtcacatgc tcgacctgcc gacggtcacg ctcggattct
     3361 ggatgctggc ggcggcggtt ccggtcgccg tggtgacgac gctcgcgttc gagacgtcgc
     3421 tgtggcgcct gccgacgccg accgagtggg ccgcgatgct ctacaacatc ctggtcgcga
     3481 tcgcgttctg ccacatcgtg tggttccatc ttgcgcgcct gctgccgccg gccgccagtg
     3541 gtctttcggt gatgctgatc ccggtggtgg gggttttttc cagcatggcg atgctcggcg
     3601 agcggccgca ctggcaggac tacaccgcgc tcgccctcat cctcgctgcg ctggcgaccg
     3661 tgctgctgcc gcagcggccg aagtaggagg cgaaaagcaa agggccagcg cttcgctggc
     3721 ccttgtttga cctgcgccga gcgcaggagg gatcggcgtc caatgcgccg aaatcgataa
     3781 gttcaacgta ctcgcgatcg cctgcatcgg caattgccgg aagttgggac gcgaaccgga
     3841 gacaccctcc gctacgcggt cacctgctgc ttgcgttccg ccaggtaacg ctgaatcaag
     3901 gcgatcagtt cgtcctcctg gtacggcttg cccaggtact cgttgacgcc gatgtcgaag
     3961 gcgagccggc ggtgcttctc ggccgtgcgc gacgtgatca tgatgatcgg gatgtcgcgc
     4021 gtagcggcat tgccgcgcac gttgcgtgtc aggtcaaagc cgtccatgcg cggcatctcg
     4081 atatcgacca gcatcagatc cgggcggttg tcctgaagct gacgcagcgc atcgacgccg
     4141 tccttggcct gcagcacctc gtagccgtgg cgctcgagca gacgctgcgt cacgcggcga
     4201 accgtcagcg aatcgtcgac taccatgacc tgcggcggtg ccgcggccgc gatctcgccc
     4261 gcatcttccg cgccgccggc gtcggcgggc agcatgaagt gcgcgtcggc gagcgtcggc
     4321 ggttccggcg cccgcatgat cagctgcacc gggttgatga tcagcacgat ctcgccgttg
     4381 ccgaggatcg tcgcgccgag cacgccggcg agccgcgaga cgctggcgcc gacgttcttc
     4441 accacgattt cctggttgtt cgacacgtcg tcgaccgcga tcgcgagccg gtcagcgccg
     4501 gacttgagca cgatcacagg aatctgcttc acgtgcgcga gcgtgatctc ctctcccagc
     4561 agttgcgcca gcggacgcag gacgatcgga ccgacgccgg ggatctcgat catgccgtcg
     4621 ccgatcgagc gcgcaagctc ggccggccgg aaccgcagca cctgctcgac catccccgac
     4681 ggcagcgcaa agcgccgctg gccgacgcgg gccagaacgg cctgcatcac cgccagcgtg
     4741 agcggcaggt acagcgtgaa gcgtgtgccg cggcccgcct ccgtgccgac cgcgatgcgg
     4801 ccgccaaatg aactgagctc cgagcgcacg acgtccatgc cgacgccgcg gccggcaagt
     4861 tcggtgacct cgtccgcggt cgagaaaccg ggcgcgaaga tcagctcgat cagctcacgc
     4921 tcgctgaccg gctggtccgg ggcgatcagc tgccgttcga tcgcccgcgc gcggatgcgc
     4981 tccaggttca gccccggacc gtcgtcggca atgacgacga tgatctcgtt ggcttcctgg
     5041 cgcatgtcga tcttcagttc gccggtttcg ggcttgccgg cggcgcgccg ctgcgcgggc
     5101 gcctcgagcc cgtgcgcgat cgcgttgcgg accaggtgct cgaacggccc gcccatcttc
     5161 tccagcacgc cgcggtcgat ctccgtattg gcgccccgga tgtcgaggtt cacgcgcttg
     5221 tcgagttcct tcgccgcctg gcgcgcgacg cgatacagcc gctcggagat actggcgaat
     5281 gtcaccagtc gcacgcgcat cagctgctgc tgcaactcgc gcgtgaggcg cccctgcgcg
     5341 gtcaggtcgg tgtcggcgag ctgcaggccc ttcaacatgt tgccctggac catcgccacg
     5401 tcttcgaccg tctcgcgcag catccgcgtc agttcctgca ggcgcgtgta gcggtcgtat
     5461 tcgagcggat cgaacgcggc cgattcgcgc gacagctggt cggagcgcgc atggatctgc
     5521 gcgtcggcct ggatctccac ctcccgcagc tgcgaacgca gccgctggat gttttcggtc
     5581 aggtcgacga tcgcgccgcg cagcgtggtg atctcgttct cgagcttggc gcgcgcgatc
     5641 gacacttcgc cggcatggtc gaccagcttg tcgagcacgt cggcgcgtac gcggatgtag
     5701 ttcgcggtct gcgccgtgac cggcgcggcc ggcgttgccg tgacctcttc ggtcgcgacc
     5761 ggcgcctcgg ccgccttgct cggggcgacg gcggcttcga tcggcgcggg cgcaggggtc
     5821 ggcgccgtgc cggactgcag gccttcgaac aggctcatcg cgcggtcgta agcaccgtgc
     5881 agatcttcga ccagagcggg cggcacgtcg gcaaggtgca tcgacgcctc gatccgcgtt
     5941 tccatgtcgt gcacgacttc gccgagccgc attgcgccgg ccatgcgcgc actgcccttc
     6001 accgtgtgca actggcgcat gagttcgcgc gccacgtcac gatcgccggg gcgcgcggcc
     6061 agcgcacgca gctgggcgcc gatcgcgggc agcaggtcac cgccttcggt caggaacacc
     6121 ggcagcaggt cggcatcgag ctcgtcgtgc actgc
//
LOCUS       DBNHBY010000491         2315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1046470, whole genome shotgun sequence.
ACCESSION   DBNHBY010000491 DBNHBY010000000
VERSION     DBNHBY010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1046470"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16HB_12110"
     CDS             complement(<1..461)
                     /locus_tag="AC16HB_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607579.1"
                     /translation="MGSVLCFTLLDTITKFTTARYPVPVLVWARYAVQFVAMLVWLGP
                     SMRFDLVRTKRLRMQVFRGMLLLASSLFFVTALRSLPLAEATSLNYTTPIIVVLMARL
                     ILHERMTRARIAFVVVGAAGMLLIVQPGTDVFRGGAVFGLAAAVFYACYQVT"
     gene            603..1046
                     /locus_tag="AC16HB_12115"
     CDS             603..1046
                     /locus_tag="AC16HB_12115"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5607580.1"
                     /translation="MSIVRIARLLSVATFAVLAAAVQVPTAAAASTDAKSRVVMQVSD
                     ADPGKWNLALNNAKNVQNDLGASNVEVEIVAYGPGIGMLKAESVVGNRIEEAMKAGVK
                     VVACENTMHGQKLTKADMLNGIAYTPAGVTEIMRRQQEGWAYLRP"
     gene            complement(1069..1142)
                     /locus_tag="AC16HB_12120"
     tRNA            complement(1069..1142)
                     /locus_tag="AC16HB_12120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1108..1110),aa:Gly,seq:ccc)
     gene            1287..>2315
                     /locus_tag="AC16HB_12125"
     CDS             1287..>2315
                     /locus_tag="AC16HB_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5607581.1"
                     /translation="MSTTRRVLVGVALLVAVGAAVTVAFRYTPGSRAEAPKAAATVPV
                     SVAKVERKTVPVRVHAIGNVEPHTTVAIKARIDGQIVAAHFKEGDEVREGQVLFEIDR
                     RPFEAQLAQAQANLAKDRALLDRANEQEKRYQDLLQQKFISQDAYAQIKTNAETAAAQ
                     VRADDAAIQAVQLQLSYCTIRAPITGYAGKIQIQQGNLVKANDTNALVVINQIVPVNV
                     SFSVPEQRLAAVRKYQADGELQVATQVPGANGPPIAGRLSFIDNTTDATTGTIRLKAE
                     FPNTDKRLWPGQFVNVVLTLHEQKDAVVTPSAALQNGPNGQYVFVVKPDQTVELRDVK
                     VARTEGDDA"
BASE COUNT          460 a          751 c          788 g          316 t
ORIGIN      
        1 gtgacctggt agcacgcgta gaagaccgcc gcggcgaggc cgaagacggc gccgccgcgg
       61 aagacgtcgg tacccggctg gacgatcagc agcatgccgg ccgcgccgac gacgacaaac
      121 gcgatgcgcg cgcgcgtcat ccgctcgtgc aggatcaggc gcgccatcag cacgacgatg
      181 atcggcgtgg tgtagttgag cgacgtcgcc tcggccagcg gcagcgagcg gagcgcggtg
      241 acgaagaaca gcgacgaggc gaggagcagc atgccgcgga acacctgcat gcgcagccgt
      301 ttcgtgcgca cgagatcgaa acgcatgctc ggcccgagcc agacgagcat cgcgacgaac
      361 tggaccgcgt agcgcgccca gacgaggacc ggcaccgggt agcgcgcggt cgtgaacttg
      421 gtgatcgtgt cgaggagcgt gaagcagagc accgagccca tgaccaggag aatcgccgac
      481 gtcgggacgt gggcatggcg cattggcgcg attatcgccg aatcgcacgt cgggcaccgg
      541 attcgcgcat aatccgcact tcgccgcgaa tgccggcgcc aaggaccaag gaggagtcac
      601 cgatgtccat tgtccggatt gcccgactgt tgtccgtcgc gacgtttgcg gtgctcgccg
      661 ccgccgtgca agtgccgacc gccgccgctg cctcgaccga cgccaagagc cgggtcgtca
      721 tgcaggtaag cgatgcggac cccggcaaat ggaatctcgc gctcaacaac gcgaagaacg
      781 tgcagaacga tctcggcgcg agcaacgtgg aggtcgagat cgtcgcctac ggtcccggca
      841 tcggcatgct gaaggccgaa tcggtcgtcg gaaatcgcat cgaggaggcg atgaaggcgg
      901 gcgtcaaggt cgttgcgtgc gagaacacga tgcacggcca gaagctcacg aaggccgaca
      961 tgctgaacgg catcgcctac acccccgccg gcgtgaccga gatcatgcgg cggcagcagg
     1021 agggctgggc gtacctgcgg ccctgaccgg ggcaggcgcc gaggaaggtg gagcgggtga
     1081 agggaatcga accctcgtat gcagcttggg aagctgccgt tctgccattg aactacaccc
     1141 gcgtcgcgct tttggccacc ggaaatcgca cggttcgatc cgcctgagcg gccggacagg
     1201 cgctcgcgct cggaccgtac gaaaatcccg ggaaaccgtg gaacattgta gcgggaatca
     1261 agccgcagcc tgacggtcct aaaaccgtgt ccaccacccg tcgcgttctg gtcggcgtcg
     1321 cgttactggt cgccgtcggc gcggcggtga ctgttgcatt tcgttacacc cctggctcac
     1381 gcgcggaagc gcccaaggct gccgccacgg tgccggtcag cgtcgcgaag gtcgagagga
     1441 agacggtgcc ggtgcgcgtg cacgcgatcg gcaacgtcga gccacacacc accgtcgcca
     1501 tcaaggcgcg catcgacggg cagatcgtcg ctgcgcactt caaggaaggc gacgaggtgc
     1561 gcgagggaca ggtcctcttc gagatcgacc gccgtccctt cgaggcgcag ctcgcccagg
     1621 cccaggcgaa cctcgcgaag gaccgtgcgc tgctcgatcg cgcgaacgag caggagaagc
     1681 gctatcagga cctcctgcag cagaagttca tctcgcagga cgcgtatgcg cagatcaaga
     1741 ccaacgccga gacggcggcc gcgcaggtgc gcgccgacga tgcggcgatc caggccgtgc
     1801 agctgcaact ctcctattgc acgattcgcg cacccatcac cggttacgcc gggaagatcc
     1861 agatccagca gggcaacctc gtcaaggcca acgacacgaa cgcgctcgtc gtcatcaacc
     1921 agatcgtgcc ggtcaatgtc tcgttctccg tgccggagca gcggcttgcc gccgtgcgca
     1981 agtaccaggc ggacggcgag ttgcaggtgg cgacgcaggt tcccggcgcg aacgggccgc
     2041 ccatagccgg caggctttcg ttcatcgaca acacgaccga cgcgacgacc gggacgatcc
     2101 ggctcaaggc cgagttcccg aacaccgaca agcgactctg gccgggccag ttcgtgaacg
     2161 tcgtgctcac gctgcacgag cagaaggacg cggtggtcac gccctcggcc gcgctgcaga
     2221 acgggcccaa cgggcagtac gtgttcgtcg tcaagcccga ccagacggtg gagctccgcg
     2281 acgtcaaggt cgcgcgcacc gagggcgacg acgcg
//
LOCUS       DBNHBY010000492         2716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1656982, whole genome shotgun sequence.
ACCESSION   DBNHBY010000492 DBNHBY010000000
VERSION     DBNHBY010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1656982"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="AC16HB_12130"
     CDS             complement(<1..345)
                     /locus_tag="AC16HB_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5607582.1"
                     /translation="MRACLAGALLALLPAPLPAGAETVTRVDVARQDSNYTVEAVMVA
                     PVPVAAAWAVMTDFDAMARFVPNLTESRVVARQGHRWTVEQKGVARFGPFRFRFESVR
                     ELDLVPTERVVSR"
     gene            complement(342..2618)
                     /locus_tag="AC16HB_12135"
     CDS             complement(342..2618)
                     /locus_tag="AC16HB_12135"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR5607583.1"
                     /translation="MSENLRDAALEYHRKPTQGKISVVPTKAMVTQRDLSLAYSPGVA
                     EACNEIVRDPGEAYALTARGNLVAVVTNGTAVLGLGNIGALAGKPVMEGKGCLFKKFA
                     GIDVFDIEVAEHDPDKLVDVIAALEPTFGGINLEDIKAPECFYIERKLRARMKVPVFH
                     DDQHGTAIVAAAAILNGLRWVKKDIGKAKLVCSGAGAAAIACLDLLVSLGMKRENIFA
                     VDSKGVLYRGRAGMDESKLAYARETSARTLADAMPGADIFMGLSGPGVLKPEMVKQMA
                     PQPIILAMANPTPEIMPEEALAVRPDAIIGTGRSDYPNQVNNVLCFPFIFRGALDVGA
                     TTINEAMKLATVRAIADLTHAEIPDEVARAYGAEGLRFGPEYLLPKPFDPRLIERIAP
                     AVAQAAMESGVASRPLADLDAYRARLRRFVYQSGSTMEPVFEAAKKAVAKRLIYCEGE
                     EERVLRAAQVVVDEGIARPILIGRPDVIAMRIERYGLRLASGRDFDTVNPLDDPRYRE
                     TWGGYYALAKRKGVTRSLAQTEVRTRSTLIGAMLVHMGEADAMLCGTVGAYPDHLKYV
                     RNVIGLRPGVNTFAAMQLLILPQRQLFICDTHVNPDPTAEQIAEMTLLAAEEVRSFGL
                     VPSVALVSHSNFGSSDLASARKMREALALILAREPDFLIDGEMQADAALSRDVLENIL
                     PDSALTAEANLLIMPNLDAANITYNALRVTAGGGITIGAILLGAAKPVHILTPAATVR
                     RIVNMSAVAVVDAAAQAM"
BASE COUNT          409 a          970 c          892 g          445 t
ORIGIN      
        1 tcgcgacacg acgcgctcgg tcggaacgag gtccagttca cgcaccgatt cgaagcggaa
       61 ccggaacggg ccgaagcgcg cgacgccctt ctgctcgacg gtccagcggt gaccctggcg
      121 ggcgacgacg cggctctcgg tcaggttcgg cacgaagcgc gccatcgcgt cgaagtcggt
      181 catcaccgcc cacgctgcgg cgaccggcac cggtgcgacc atcaccgctt ccacggtgta
      241 gttcgagtcc tgccgcgcga cgtcgacccg cgtgaccgtt tccgcgcccg ccggcagcgg
      301 tgccggcagc agcgcaagca gcgccccggc gagacatgcc ctcacatcgc ctgcgccgcc
      361 gcgtcgacca ccgcgacggc cgacatgttg acgatccgcc gcaccgtcgc cgccggcgtc
      421 aggatgtgca ccggcttggc cgcgccgagc aggatcgcgc cgatggtgat gccgccgccg
      481 gccgtcacgc gcagcgcgtt gtaggtgatg ttggcggcgt cgaggttcgg catgatcagc
      541 aggttcgcct cggcggtcag cgccgagtcc ggcaggatgt tctccagcac gtcgcgcgac
      601 agcgccgcgt cggcctgcat ctcgccgtcg atcaggaaat cgggctcgcg tgccaggatc
      661 agcgcgagcg cctcgcgcat cttgcgcgcg ctcgccagat ccgagctgcc gaagttcgag
      721 tgcgacacca gcgccacgct cggcaccagg ccgaaactgc gcacctcctc ggccgcgagc
      781 agtgtcatct cggcgatctg ctcggcggtc gggtccgggt tcacgtgcgt gtcgcagatg
      841 aacagctgtc gctgcggcag gatcagcaac tgcatcgcgg caaaggtgtt cacgcccggc
      901 cgcagtccga tcacgttgcg cacgtacttc aggtgatcgg gataggcgcc gaccgtgccg
      961 cacagcatcg catcggcctc gcccatgtgc accagcatcg cgccgatcag cgtcgagcgc
     1021 gtacgcacct cggtctgcgc cagcgagcgc gtgacaccct tgcgcttggc gagcgcgtag
     1081 tagccgcccc aggtctcgcg gtagcgcgga tcgtcgagcg gattgacggt gtcgaaatcg
     1141 cgcccggacg cgagccgcag cccgtagcgc tcgatgcgca tcgcgatcac atcgggccgc
     1201 ccgatcagga tcggccgcgc gatgccctcg tccaccacga cctgcgcggc gcgcagcacg
     1261 cgctcctcct cgccctcgca gtagatcagg cgtttggcga cggccttctt cgccgcctcg
     1321 aacaccggct ccatcgtgct gccggactgg tagacgaagc ggcgcagccg cgcgcggtac
     1381 gcgtcgaggt ccgccagcgg gcgcgaggcg acgccgcttt ccatcgctgc ctgcgctacg
     1441 gccggcgcaa tgcgctcgat cagcctggga tcgaacggct tgggcagcag atattcggga
     1501 ccgaagcgca gtccttcggc gccgtaagcg cgcgccacct cgtccggaat ctccgcatgc
     1561 gtcagatcgg cgatcgcgcg cacggtcgca agtttcatcg cctcgttgat cgtggtggca
     1621 ccgacgtcga gcgcgccccg gaagatgaag gggaaacaca ggacgttgtt gacctggttc
     1681 ggataatcgg agcggccggt gccgatgatc gcgtcgggac gcaccgccag cgcttcctcc
     1741 ggcatgatct ccggcgtcgg gttggccatc gccaggatga tcggctgcgg cgccatctgc
     1801 ttgaccatct cgggcttcag cacaccgggg cccgacagcc ccatgaagat gtcggcgccc
     1861 ggcatcgcgt cggccagcgt gcgcgccgag gtctcccgcg cataggcgag cttgctctcg
     1921 tccatgccgg cgcggccgcg atacagcacg cccttgctgt cgaccgcgaa gatgttctcg
     1981 cgcttcatcc cgaggctcac cagcaggtcc aggcacgcga tcgccgccgc gccggcaccg
     2041 gagcagacta gcttcgcctt gccgatgtct ttcttcaccc agcgcaggcc gttcaggatc
     2101 gcggcggcgg cgacgatcgc ggtgccgtgc tggtcgtcgt ggaacaccgg caccttcatc
     2161 cgcgcgcgca gcttgcgctc gatgtagaag cactccggcg ccttgatgtc ctccaggttg
     2221 atgccgccga aggtcggctc gagcgcggcg atgacgtcga ccagcttgtc cggatcgtgt
     2281 tcggcgacct cgatgtcgaa cacgtcgatg ccggcgaact tcttgaacag gcagcccttg
     2341 ccctccatca ccggcttgcc cgcgagcgcg ccgatgttgc ccagccccag caccgcggtg
     2401 ccgttggtga cgaccgcgac caggttgccg cgtgcggtca gcgcataggc ctcgcccgga
     2461 tcgcgcacga tctcgttgca cgcctcggcc acacccggcg agtatgcgag cgacaagtcg
     2521 cgctgcgtca ccatcgcctt ggtcggcacg accgaaatct tgccctgcgt cggtttacgg
     2581 tgatattcaa gcgcggcgtc gcgcaggttc tctgacattt gagtcccctc gatatacgca
     2641 cgccgtcgcg cgggccctgc gtccgcgctc gccgtgggag tcgtagatta cgccggcttc
     2701 agtcgtagtt cgaccc
//
LOCUS       DBNHBY010000493         3282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1790523, whole genome shotgun sequence.
ACCESSION   DBNHBY010000493 DBNHBY010000000
VERSION     DBNHBY010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1790523"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="AC16HB_12140"
     CDS             complement(<1..414)
                     /locus_tag="AC16HB_12140"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HIR5607584.1"
                     /translation="MQRYESPDLVVEYDPQRCIHAAECVHGLPAVFDSKARPWIRLGN
                     TSAAETAAVIARCPTGALTARRPDGSAVEQPDAVNTVTIVPDGPLYVRGRIAHAGAEF
                     TRVALCRCGASANKPFCDKSHEKSGFKHAGDCHARA"
     gene            complement(423..1901)
                     /locus_tag="AC16HB_12145"
     CDS             complement(423..1901)
                     /locus_tag="AC16HB_12145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR5607585.1"
                     /translation="MLQRVSSLPPLTDTTLVKTQAFIDGAWVEGGQGKFAVLNPADGG
                     QLVEVANCGPAEAQRAIDAAAKAFPAWRAKTAKERAAILRKWFELMMAAQEDLAKLMT
                     AEQGKPLAESRGEIGYGASFVEWFAEEGKRIYGDTIPTPWGDRRLFAFKEPIGVCAAI
                     TPWNFPNAMITRKCAPALAAGCTVVVKPAEQTPLSALAAAELALRAGMPPGVFNVVTA
                     DEKQSIAVGKVLCESPIVRKLSFTGSTQVGRILMQQCAPTLKKLSLELGGNAPFIVFE
                     DADVDAAVEGALLSKYRNTGQTCVCTNRFFAHAKIYDEFVRKLAVKAETLKVGSGFDA
                     GVQQGPLIDANALAKVEAHVADAVAKGATVLTGGRRHERGGTFYVPTVLANVTKEMLV
                     AREETFGPVAPVFRFESDDEAIAAANSVEYGLASYFYSRDVGRILRVAERLEYGMVGV
                     NAGIISTEVAPFGGVKQSGFGREGSYQGIDEYVVTKYVCIGV"
     gene            1993..2892
                     /locus_tag="AC16HB_12150"
     CDS             1993..2892
                     /locus_tag="AC16HB_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR5607586.1"
                     /translation="MQFESLQSDLKNWLKTSVEGGHGRETLIESLRVAGYQPDFARRA
                     VEVALARFGAAAAPAPIAATVSVATGATAIPTASQLLTETTNAIATSDRNIDILFVLN
                     APRVVLFGNLLGPEECDQLIELSRRKLARSTVVNAATGAYDVHPDRTSSGTHFERGEN
                     ELITRIERRIAELIDLPIENGEPIQILHYTPGAEYKPHWDYFDPAHSGNEKVLAMGGQ
                     RVATLVMYLNDVESGGSTVFPDVGLDVLPRKGNAVYFAYTTEDGQLDRRTLHGGSPVI
                     AGEKWIATKWLRQREYRALLAAA"
BASE COUNT          533 a         1118 c         1088 g          543 t
ORIGIN      
        1 cgcgcgcgcg tggcagtcgc cggcgtgctt gaagccggac ttctcgtggc ttttgtcgca
       61 gaaaggcttg ttcgcactcg caccgcagcg gcagagcgca acgcgcgtga attcggcacc
      121 ggcgtgcgcg atgcggccgc gcacgtacag cggtccgtcg ggcacgatcg tcacggtatt
      181 gaccgcgtcg ggctgctcga ccgcgctgcc gtcgggtcgg cgcgcggtca gggcgccggt
      241 cgggcagcgg gcaatgaccg cggcggtctc cgcagcactc gtgttgccca gccggatcca
      301 cggccgtgcc ttcgaatcga acaccgcagg caggccgtgc acgcactcgg cggcgtggat
      361 gcagcgctgc ggatcgtatt cgacgacgag gtcgggcgat tcgtagcgtt gcacggccat
      421 ggtcagaccc cgatgcacac gtacttcgtg accacgtatt cgtcgatgcc ctggtacgaa
      481 ccctcgcggc cgaagcccga ctgcttgacg ccgccgaagg gcgcgacctc cgtcgagatg
      541 atgccggcgt tgacgccgac catgccgtac tcgagtcgct cggccacgcg caggatgcgg
      601 ccgacgtcac gcgagtagaa gtacgaggcc agcccgtact cgacgctgtt ggcggcggcg
      661 atcgcttcgt cgtcggactc gaagcggaag acgggcgcca ccggcccgaa cgtctcttcg
      721 cgcgcgacca gcatctcttt cgtcacgttg gcgagcacgg tcggcacgta gaacgtaccg
      781 ccgcgctcgt gccggcgacc cccggtgagc acggtcgcgc ccttggcgac ggcgtcggca
      841 acatgcgcct cgaccttggc gagcgcgttg gcgtcgatca gcggtccctg ctgcacgccg
      901 gcgtcgaagc cgctgccgac tttcagcgtc tcggccttca cggccagctt gcgcacgaat
      961 tcgtcgtaga tcttcgcgtg cgcgaaaaag cggttggtgc acacacaggt ctggccggtg
     1021 ttgcggtact tcgacagcag cgcaccctcg accgcggcgt cgacatcggc atcctcgaac
     1081 acgatgaacg gcgcgttgcc gcccagttcg agcgacagct tcttcagcgt cggcgcgcac
     1141 tgctgcatca ggatgcggcc cacctgcgtc gacccggtga acgaaagctt gcgcacgatc
     1201 ggcgattcgc acagcacctt gcctaccgcg atcgactgct tctcgtcggc ggtcacgacg
     1261 ttgaacacgc cgggcggcat gccggcgcgc agcgccagtt ccgcggcggc gagcgccgac
     1321 agcggcgtct gctcggcggg cttgaccacg accgtgcagc cggccgcgag cgccggcgcg
     1381 catttgcgcg tgatcatcgc gttgggaaag ttccagggcg tgatcgccgc acagacgccg
     1441 atcggctcct tgaacgcgaa caatcgccgg tcaccccacg gcgtcgggat cgtgtcgcca
     1501 tagatgcgct tgccttcctc ggcgaaccac tcgacgaagc tcgcgccgta gccgatctcg
     1561 ccgcgcgatt ccgccagcgg cttgccctgc tcggcggtca tcagtttcgc gaggtcctcc
     1621 tgcgccgcca tcatcagttc gaaccacttg cgcagaatcg cggcgcgctc cttggcggtc
     1681 tttgcgcgcc acgccgggaa cgccttggct gccgcatcga tcgcgcgctg tgcctcggcc
     1741 gggccgcagt tggcgacttc gaccagttgg ccgccgtcgg ccggattgag caccgcgaac
     1801 ttgccctggc cgccctcgac ccaggcgccg tcgatgaagg cctgtgtctt caccagcgtg
     1861 gtgtcggtca gcggcggaag cgatgaaacc cgttgcagca tggcaacctc cgtgtgattt
     1921 cgcccaaaga atagcgcgcg gcccgcgccg tgccacggcg gtatcctcac gcaagcgcgg
     1981 gcgggagacc ccatgcaatt cgagtcgctg caatccgatc tgaagaactg gctcaagacg
     2041 tccgtcgaag gtggccacgg gcgcgagacg ctgatcgagt cgctgcgcgt ggccggctat
     2101 cagccggact tcgcgcgccg ggcggtcgaa gtggcgctgg cgcgcttcgg tgcggccgcc
     2161 gcgcccgctc cgatcgccgc gacggtgtcc gtggcgacgg gtgcgacggc gataccgacc
     2221 gcgagtcagc tgctgacgga gaccaccaac gcgatcgcga cgtcggaccg gaacatcgac
     2281 atcctgttcg tgctcaacgc gccacgggtg gtcctgttcg gcaacctgct ggggccggag
     2341 gaatgcgacc aactgatcga actgtcgcgt cgcaaactgg cgcgctcgac ggtcgtgaac
     2401 gcggccaccg gcgcctacga cgtgcacccg gaccgcacca gcagcggcac gcacttcgag
     2461 cgcggcgaga acgagctgat cacacgaatc gaacgtcgca tcgccgagtt gatcgacctg
     2521 ccaatcgaaa atggcgagcc gattcagatc ctgcactaca cgcccggcgc cgaatacaag
     2581 ccgcactggg actacttcga tcccgcgcac tccggcaacg agaaggtgct ggcgatgggc
     2641 ggccagcgcg tggccacgct ggtgatgtac ctcaacgacg tggagtcggg cggctccacc
     2701 gtgttccccg acgtcggcct cgacgtgctg ccgcgcaagg gcaacgcggt ctacttcgcc
     2761 tacacgaccg aggacggcca actcgaccgc cgcacgctgc atggcggcag cccggtcatc
     2821 gcgggcgaga agtggattgc gaccaaatgg ctgcggcagc gggagtaccg cgcgttgctg
     2881 gcggccgcct gatcgcgcgt ggctcttggc gcggcgcagc cacatttgcg cctcggtgcc
     2941 aggcaacgtc ggttgtgggt cgtgaaggat tcgaaccttc gaccagcgga tgaagagatc
     3001 cgatcagtcg tttgttggcg attgaaccac tgctcgtgct cgaagagtgt cccgcaatgg
     3061 cgggcgcccc cttggcgcga cgcaccgagc cggtctcagg aggtgtggcc tgcaccatgt
     3121 ggtcttgctt cgctgactcc gaaaagtgaa gttgccactt cggttcccgt ccaaaggcct
     3181 acgaagggcg cgcagatcgg taaagcggtg tgcccttcca gcgtccatca ttcaccgcct
     3241 caattgcgag tcgcttgacg acctcgtaaa cagcctgcgc cg
//
LOCUS       DBNHBY010000494         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1724539, whole genome shotgun sequence.
ACCESSION   DBNHBY010000494 DBNHBY010000000
VERSION     DBNHBY010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1724539"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="AC16HB_12155"
     CDS             complement(<1..925)
                     /locus_tag="AC16HB_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004539897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR5607587.1"
                     /translation="MTVRIIDVFNGDADGICALHQLRLAEPAESELVTGLKREIELLA
                     RVDAGAGARIAVLDIALARNRAALDRLLARGAHVRWFDHHYAGTVPAHPQFEARIDTA
                     PDVCTSLIVDRHLGGRFTHWAIAAAFGDNLIDVAQARAAALGLDADATLRLRALGEAL
                     NYNSYGDEAADVLMHPRALYLRLRPHADALEFARSDAIVAALIERRREDLARAAALAP
                     LAANEGSAVFELPNEAWCRRVLGTFAHELANGQQRRAHAVLKDNGDGTCAVSVRAPQS
                     SRHGADVLCRRFGGGGRAGAAGIDRLPAAERE"
     gene            complement(922..2514)
                     /locus_tag="AC16HB_12160"
     CDS             complement(922..2514)
                     /locus_tag="AC16HB_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside
                     phosphotransferase/ATP-binding protein"
                     /protein_id="HIR5607588.1"
                     /translation="MNDDALARHRELVAALMRPAAYAHPVQSVELIETHISSVLLAGD
                     YAYKIKKPVNLGFVDFSTLAQRRFCCFEEVRLNRRSAPQLYLGVEPITGSGAQPRMGG
                     EGEALDYAVRMRRFDSANLLDARARRGELSAPQIDRLAQAIGDFHVAAARASPPSGFG
                     MPEQVAAWARENFAQLQALPQQADVAAALQRLAEWTEREYERCAPNLAQRLPGGFVRE
                     CHGDLHLGNIVLLDDVPTLFDAIEFNPQLRWIDVVSDLAFAFMDLFDHGLPQFAWRLL
                     DRYLQVTGDYDGLDLLRFYVVYRALVRSKVARIRAEQPHVTDDERAAALTRCAHYVRL
                     AEGRAQVGGRPLLAITFGLSGSGKTTVASELLQHLGAVRVRSDIERKRMFGIDETARM
                     AGDTLAALYSADATRRTYERLGEIARGILAAGYPALIDAAFLKRAERDEFRVLARALG
                     ARFALIECTSAPAVLRARVAQRMERAADASDATVAVLELQLDGHEPIAADERAETISI
                     ATDVDAPRLAARCAEVAARLAT"
     gene            2637..>2908
                     /locus_tag="AC16HB_12165"
     CDS             2637..>2908
                     /locus_tag="AC16HB_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR5607589.1"
                     /translation="MKLPLQITFQDITPSEALDRAVRERAARLDQFHPHILSCRVVIA
                     RETHRRHQGNEFSVRFDIKVKGHEVAITRNADEDPFVALRDAYDAAR"
BASE COUNT          438 a         1066 c         1000 g          404 t
ORIGIN      
        1 attcacgttc cgcggcaggc aggcgatcga ttccggcagc gccggcgcgc ccgccgccgc
       61 cgaaccgccg gcacagcaca tcggcgccgt gccgcgacga ctgcggcgca cggacgctca
      121 ccgcgcaggt gccgtcgccg ttgtccttca gcaccgcgtg cgcgcgccgc tgttggccgt
      181 tggccagttc gtgcgcgaac gtgccgagca cgcgccggca ccacgcctcg ttcggcagct
      241 cgaataccgc gctgccctcg ttcgcggcga gcggcgcgag cgcggcggcg cgcgcgaggt
      301 cttcccggcg gcgctcgatc agcgcggcga cgatcgcatc gctgcgcgcg aactcgagcg
      361 cgtccgcgtg cgggcgcagc cgcaggtaca gcgcgcgcgg atgcatcagc acgtcggccg
      421 cttcgtcgcc gtagctgttg tagttcagcg cctcgccgag cgcgcgcagc cgcagcgtcg
      481 catcggcgtc gagcccgagc gcggcggcgc gcgcctgcgc gacgtcgatc aggttgtcgc
      541 cgaacgcggc ggcgatcgcc cagtgggtga agcggccgcc aagatggcga tcgacgatca
      601 ggctcgtgca cacgtcgggc gcggtatcga tccgcgcttc gaactgcgga tgcgcgggca
      661 cggtgccggc gtagtgatga tcgaaccagc gcacgtgcgc gccgcgcgcg agcagccggt
      721 cgagcgcggc gcgattgcgc gccagcgcaa tgtcgagcac ggcgatgcgc gcgccggcac
      781 cggcatcgac gcgcgccagc agctcgatct cgcgcttcag ccccgtcacc agctccgact
      841 cggcgggctc ggccaggcgc agctgatgca gcgcgcagat gccgtcggca tcgccgttga
      901 acacgtcgat gattcgcacc gtcacgtcgc cagtcgcgcc gcgacctcgg cgcaacgcgc
      961 cgccagccgc ggcgcatcga catcggtcgc gatgctgatc gtctcggcgc gctcgtcggc
     1021 ggcgatcggc tcgtggccgt cgagctgcag ctccagcacc gcgaccgtgg cgtcggacgc
     1081 gtcggccgcg cgctccatcc gctgcgccac gcgcgcgcgc agcaccgccg gcgcggaagt
     1141 gcactcgatc aacgcgaagc gtgcgccgag cgcgcgcgcc agcacgcgaa actcgtcgcg
     1201 ctcggcgcgt ttcaggaacg cggcgtcgat cagcgccgga taaccggcgg cgaggatccc
     1261 gcgcgcgatc tcgcccagcc gctcgtaggt gcgccgtgtc gcatcggccg aatacagcgc
     1321 ggccagcgtg tcgcccgcca tgcgcgcggt ctcgtcgatg ccgaacatgc gcttgcgctc
     1381 gatgtccgat cgcacccgca ccgcgccgag gtgctgcagc agctcgcttg cgaccgtcgt
     1441 cttgccgctg ccggacagcc cgaacgtgat cgcgagcagc ggccggccgc cgacctgcgc
     1501 gcggccttcg gccagccgca cgtaatgcgc gcagcgcgtc agcgccgccg cgcgctcgtc
     1561 gtccgtgacg tgcggctgct cggcgcggat gcgcgcgacc ttggaccgca ccagcgcgcg
     1621 gtacacgaca tagaagcgca gcaggtccag accgtcgtag tcgccggtca cctgcaggta
     1681 gcggtcgagc agccgccacg cgaactgcgg cagtccgtgg tcgaacagat ccatgaacgc
     1741 gaacgcgaga tcgctcacga cgtcgatcca gcgcaactgc gggttgaact cgatcgcatc
     1801 gaacaacgtc ggcacgtcgt ccagcagaac gatgttgccc aggtgcaggt cgccgtggca
     1861 ctcccgcacg aagcccccgg gcagccgctg cgccaggttt ggtgcgcagc gctcgtactc
     1921 gcgctcggtc cactcggcca gccgctgcag agccgcggcg acgtccgcct gctgcggcag
     1981 cgcctgcagc tgcgcgaagt tctcgcgcgc ccacgccgcg acctgctcgg gcatcccgaa
     2041 tccggacggc ggcgaagcgc gcgccgccgc cacgtggaag tcgccgatcg cctgcgccag
     2101 ccggtcgatc tggggcgcgc tcagttcacc gcgccgcgcg cgcgcatcga gcaggttggc
     2161 gctgtcgaag cggcgcatgc gcaccgcgta gtcgagcgcc tcgccctcgc cgcccatgcg
     2221 cggctgcgca ccgctgccgg tgatcggctc cacgccgaga tacagctgcg gcgcgctgcg
     2281 acggttcagc cgcacttcct cgaagcagca gaagcggcgc tgtgcgagcg tggagaagtc
     2341 gacgaaaccg aggttgaccg gcttcttgat cttgtacgca tagtcccccg ccagcagcac
     2401 cgacgagatg tgcgtctcga tcagttccac gctttgcacc ggatgcgcgt acgccgctgg
     2461 ccgcatcagt gcggcgacca gttctcgatg gcgggccaat gcgtcgtcgt tcatcgcgga
     2521 accacgcgcc tcattgtgct caccccgctg ccgccgccga tttgacaggc gtcaatgagc
     2581 acatgcgcgc gcggcctacg gtgaagacgc gacaaacggg aacgaaagga gtgcagatga
     2641 aacttccgct gcagatcacg ttccaggaca tcacgccgag cgaggcgctg gaccgcgcgg
     2701 tgcgggagcg ggcggccagg ctcgatcagt ttcatccgca cattctgagc tgccgcgtgg
     2761 tgatcgcccg cgagacgcac cgccgccatc agggcaacga gttctcggtg cgtttcgaca
     2821 tcaaggtcaa gggccacgaa gtcgcgatca cccgcaacgc ggacgaagat ccgttcgtcg
     2881 cgctgcgcga cgcctacgat gccgcccg
//
LOCUS       DBNHBY010000495         6343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_303509, whole genome shotgun sequence.
ACCESSION   DBNHBY010000495 DBNHBY010000000
VERSION     DBNHBY010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_303509"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16HB_12170"
     CDS             complement(<1..464)
                     /locus_tag="AC16HB_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR5607590.1"
                     /translation="MNKIGRRTARGFTLLELLVAITVLSLVSLISWRGLESLTATRLR
                     LEPEVDDLRATLTVFGQMELDLAQVARPVFVPLPANPISTTGGDQASLQIIRFAPIDR
                     DEASAIQRVVYSLKDGQLTREVSAPIRTPGLLAQAPLTEAKLLANVRAMQVRV"
     gene            complement(461..829)
                     /gene="gspI"
                     /locus_tag="AC16HB_12175"
     CDS             complement(461..829)
                     /gene="gspI"
                     /locus_tag="AC16HB_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HIR5607591.1"
                     /translation="MRSRGFTLLEVLVALVIIGVALAAAMRGALSLTGNSADARRMLL
                     ASIAAENRLLELRLARAQLSIGQTTDDCSEGGVTFTCEQNVKSTPNPFFRRVELRVTF
                     RDGDSQRRYAELMAILPINQ"
     gene            complement(832..1344)
                     /locus_tag="AC16HB_12180"
     CDS             complement(832..1344)
                     /locus_tag="AC16HB_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5607592.1"
                     /translation="MRHAFALPSSEHGFTLLEMLVVIVLAGILLAVVSVNTTPDPRQQ
                     LEREARRIGQLMSIAAEESRIRSERVYWEADLRGYRFFTASDGERQLLTDDVLRERAW
                     ERPLEQLAIYEGDSARPSQIILAADAPPVRLPVAREWVQPRWRLELASDVGRVAVEFD
                     PAGRTKVLEQ"
     gene            complement(1357..1809)
                     /gene="gspG"
                     /locus_tag="AC16HB_12185"
     CDS             complement(1357..1809)
                     /gene="gspG"
                     /locus_tag="AC16HB_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HIR5607593.1"
                     /translation="MSNSPLYQERSCRGFTLIELMVVIAILGILAALIVPKFVGFTDD
                     ARVSAAKQDIASIMQALKIYRIDNGTYPTTEQGLRALITKPTIEPIPPNWRQGGYLER
                     NSVPVDPWKREYKYLNPGLHGEIDVFSFGRDGQSGGDGYDADIGSWSL"
     gene            2019..2669
                     /locus_tag="AC16HB_12190"
     CDS             2019..2669
                     /locus_tag="AC16HB_12190"
                     /inference="COORDINATES: protein motif:HMM:NF022800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR5607594.1"
                     /translation="MKKIPTLLLHLAMLAIVCAIGAYWVMRILTPAPSAPPPPVAAPA
                     PREADPTLAARMFGLVQAAPSVASNVQVMGVFSAGKDSSAVLAVDGKAARVVLLGQSV
                     SPGSKLAEVRPDGVTLDNNGVKQELKLPPRTPIAVSGPPAPAGFSRDGNTLAAPGAGA
                     PTVPPNLSPGMPAPGFTPGFPAPPGAPLRPAQQPTQQQPELPVQSQPGGAPALPSQ"
     gene            2792..4777
                     /locus_tag="AC16HB_12195"
     CDS             2792..4777
                     /locus_tag="AC16HB_12195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5607595.1"
                     /translation="MARGVLLSERADGCAGTGRFRRWSIRARPRRPWRGLSHAVANEP
                     VPVHSSARAIRFAREAARGTPAGNGEASRIAALGRPRCGRGLASARRLADPARGRGRV
                     GSAAPRTRELHFSVRFLQFIAPHSFQRGGILTVQLERGGVLASRHGAADNECATRKDW
                     AMSDSGGGQSAPMASGAARVRQAVDRLAAGAAGAARAHAPAAKRAAARFGDWLARIGW
                     AKFFLLSILLLILGGIATSLLLKRSPAVVIEKGDPKDRVNVIVSVGPDGVKIAPPRPP
                     AVPAPPRPPVPPSVDGKATEPPAPPKASSGVKVDGKGVRILTEKDGKRVSVIVDGKGV
                     RIEDSVPPRADSKGEIAAEEAITISPQVLADADKATEAVDAARERIEEIVSEQIDRQV
                     ARRTRAYREQSSDWIMTFIMLMIVTMIIVKIVLGSKRKAETRAQAATATAAEESLKRQ
                     LAEAQLKMMQAQVEPHFLFNTLASVDYLIETDPARASTMQKNLIQYLRAALPQMREGS
                     TTLGKEIALCRSYLEILKVRMEDRLQYAITVPQGLASAQFPPMMLQSLVENSIKHGLE
                     PKAEGGTLTISADVANGNLRVTVADTGLGFAAGGRPGTGVGLSNVQERLAALYNGAAK
                     LVIEANTPSGTISTIEVPYTIDVASEPSPAAPAAQPA"
     gene            4806..5585
                     /locus_tag="AC16HB_12200"
     CDS             4806..5585
                     /locus_tag="AC16HB_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5607596.1"
                     /translation="MTRAIIADDERLMREQLRSRLAEVWPELQIVAEARNGREAVDVV
                     REYGPDLAFLDIRMPGMTGVEAAREIGDACHIVFITAYSEYAVEAFEQGAVDYVLKPA
                     DRDRLEVTVARLRKRLGEKPADLSALLNKLTLRLDGIAAPASASAAGESKHLQWIQAS
                     MGQQLRMIPVADVLFFTSDEKYTRVQTESFEALIRKPIKELVEELDPAQFWQIHRSTI
                     VNVKAIAGITRDFRGRQLVQLRNHSERLEVSRNYTHLFKQM"
     gene            5791..5876
                     /locus_tag="AC16HB_12205"
     tRNA            5791..5876
                     /locus_tag="AC16HB_12205"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5825..5827,aa:Tyr,seq:gta)
     gene            5962..6035
                     /locus_tag="AC16HB_12210"
     tRNA            5962..6035
                     /locus_tag="AC16HB_12210"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5994..5996,aa:Gly,seq:tcc)
     gene            6070..6144
                     /locus_tag="AC16HB_12215"
     tRNA            6070..6144
                     /locus_tag="AC16HB_12215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6102..6104,aa:Thr,seq:ggt)
     gene            6217..>6343
                     /locus_tag="AC16HB_12220"
     CDS             6217..>6343
                     /locus_tag="AC16HB_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5607597.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTLVLSKKYGG"
BASE COUNT         1145 a         1976 c         2089 g         1133 t
ORIGIN      
        1 acgcgaacct gcatcgcgcg cacgttggca agcagttttg cttccgtcag tggcgcctgc
       61 gccagcaatc ccggcgtcct gatcggcgcc gacacttcgc gcgtcaactg cccgtccttc
      121 agtgaataga cgacgcgctg gatcgccgag gcttcgtcgc gatcgatcgg cgcgaagcgg
      181 atgatctgca ggcttgcctg gtcgccgcca gtggtgctga tcgggttcgc cggcagcggc
      241 acgaacaccg gacgggcgac ctgcgcaaga tcgagctcca tctggccgaa gaccgtgagc
      301 gtggcgcgca gatcgtcgac ttcgggctcc agcctcagcc tggtcgcagt cagcgactcc
      361 aatccgcgcc aggaaatcag cgacacgagc gacagcacgg tgatcgcgac cagcagttcg
      421 agcagggtga agccgcgtgc cgtgcgccgc ccgatcttgt tcattggttg atcggcagaa
      481 tggccatcag ttcggcgtag cggcgctgcg aatcgccgtc ccggaaggtg acgcgcagct
      541 cgacgcgccg gaagaacggg ttcggcgttg atttcacgtt ctgctcgcac gtgaatgtca
      601 cgccgccttc gctgcagtca tcggtcgtct ggccgatcga cagctgcgcg cgcgccagac
      661 gcagttcgag caggcggttc tcggcggcga tcgacgccag cagcatgcgc cgcgcatcag
      721 ccgagttgcc ggtcagcgac agtgcaccac gcatcgccgc ggcgagcgcc acgccgatga
      781 tcaccagcgc gacgaggact tcgagcaacg tgaagccgcg cgatcgcatc gctactgttc
      841 gagcactttg gtgcgtccgg cgggatcgaa ttccaccgcg acgcgcccga catcgctcgc
      901 cagttccagc cgccagcgtg gctgcaccca ctcccgtgca accggcaggc gcacgggcgg
      961 ggcatcggcc gcgaggatga tctgacttgg ccgcgccgag tcgccctcgt agatcgccag
     1021 ctgctcgagc ggtctctccc aggcacgttc gcgcagtaca tcgtcggtca gcagttgccg
     1081 ctcgccatcg cttgcagtga agaatcgata gccgcgcaga tcggcctccc agtagacacg
     1141 ctcgctgcgg atgcgcgact cctccgcggc gatactcatg agctgtccga tccgtcgtgc
     1201 ctcgcgttca agctgctggc gcggatccgg cgtggtgttt acgctcacga ccgcgagcag
     1261 gatgcccgcc agcacgatca cgaccagcat ctccagcagc gtgaagccgt gttcgctcga
     1321 gggaagggcg aaggcatgcc gcatgcatgt gcgtggttac aggctccacg aaccgatgtc
     1381 ggcgtcgtag ccgtcgccac cggactggcc gtcgcgcccg aagctgaaca cgtcgatttc
     1441 gccatgcaag cccgggttca ggtatttgta ttcgcgcttc caggggtcga ccgggacgga
     1501 attgcgctcg aggtagccgc cctggcgcca gttgggcgga atcggttcga tggtgggttt
     1561 ggtgatcagc gcgcgcagac cctgctcggt cgtcggatag gttccgttgt cgatccggta
     1621 gatcttcagc gcttgcatga tcgacgcgat gtcctgcttg gcagcgctga ctctggcgtc
     1681 gtccgtgaaa ccgacgaact tgggcacgat cagcgcggcg agaatgccga gaatcgcgat
     1741 cacgaccatc agttcgatca gcgtgaaccc gcgacaactg cgttcctggt aaagaggtga
     1801 gttcgacatg gatgtcctga aggtgcgcgc cgcgggcgct gtacgccgga tggattctct
     1861 ggccggcata ggtcgcatcg cataatatca acgcggcgct gcggatttgg ccccgccgcg
     1921 cgcgtgagtt agcccgtgca gatgacagaa gaattcggag cgcgggcgga ctcagaatcc
     1981 gacctgcagg ccactacaac gatcgactca gacccggcgt gaagaagatc ccgaccttgc
     2041 tgctgcacct tgcaatgctc gcgatcgtct gtgcgatcgg cgcttactgg gtgatgcgga
     2101 tcctgacgcc agcgccgagt gcgcccccgc cgccggtggc ggcgccggca ccgcgcgaag
     2161 ccgatccgac gctcgcagcg cggatgttcg ggctcgttca ggctgcacca agcgtggcat
     2221 cgaacgtaca agtgatgggc gtcttttccg ccggcaagga ttcgtctgcc gtcctcgccg
     2281 ttgacggcaa ggccgcgcga gtcgttctgc tgggtcagtc cgtttcgccc ggttcgaagc
     2341 tcgcagaggt acgtcctgat ggcgtgacac tcgacaacaa cggcgtgaag caagagttga
     2401 aactgccgcc gcggacgccg attgcagtga gtggaccgcc ggcaccggcc gggttcagcc
     2461 gcgatggcaa caccttggct gcgcccggcg caggtgcgcc cacggtgccg cccaatctgt
     2521 cccccggaat gcctgcgccc ggctttaccc ccgggttccc cgcaccgccg ggcgcgccgt
     2581 tgcggccggc gcaacaaccg acccagcagc agcctgaact gccggttcaa tcgcagccgg
     2641 gcggggcgcc cgcactgccg tcgcaatagt ccgggggctg cacttcgctt cgacgcttga
     2701 cttgcgtcgc gtcggcacca ttttcgagta gcaaactgag tgcaatggcc ccgcctccgg
     2761 tacgactcca acgatctgcg cacgagcgca cgtggcgcgg ggcgttcttc tatctgagcg
     2821 cgctgatggg tgcgctggca ctggtcgatt tcgacgctgg tcgattcgcg cacggcctcg
     2881 gcgaccttgg cgtggcctgt ctcatgctgt cgctaatgaa ccagttcccg ttcattcaag
     2941 tgctcgtgca atccgatttg cacgcgaagc cgcgcgagga actcctgcgg gaaatggaga
     3001 agcttcgcgc atcgcagcct tgggtcgacc gcgttgcggc cgcgggctgg cttctgctcg
     3061 tcgcctcgct gatcctgcgc gcggccgggg ccgggtaggc agtgccgcgc ctcggacgcg
     3121 ggaattgcat ttcagtgtcc ggtttctgca gttcatcgcg ccgcactcgt tccagcgcgg
     3181 tggcatcctt acagtccaat tggaacgcgg cggggtgctc gcatcgaggc acggcgctgc
     3241 cgacaatgag tgcgcaacgc ggaaagattg ggcaatgagc gacagcggcg gtggtcaatc
     3301 cgcaccgatg gcaagcggcg cggcgcgcgt ccggcaagcc gtcgatcgat tggccgccgg
     3361 cgctgcgggc gcggcccgag cgcatgcgcc cgcggcaaag cgggcggcgg ccagattcgg
     3421 cgattggctc gctcgaatcg gctgggccaa gttcttcctg ctgtcgattc tcttgctgat
     3481 cctgggcggc atcgccacat cgctgcttct caagcggtcc cctgcggtcg tgatcgagaa
     3541 aggcgacccc aaggatcggg tcaacgtgat cgtctccgtg gggcccgacg gcgtaaagat
     3601 tgcgccgccg cggccgcctg cggtcccggc acctccaagg ccgcccgtgc cgccttcggt
     3661 cgacggcaag gcgacggagc cgccagcacc gccgaaagcc tcttcaggtg tcaaggtcga
     3721 cggcaagggt gtgcgcatcc tgacggagaa ggacggcaag cgcgtctccg tcatcgtcga
     3781 tggcaagggc gtgcggatcg aggactcggt gcccccgcgc gccgacagca aaggcgagat
     3841 tgctgcggaa gaggcgatca cgatttcgcc ccaggtactg gccgatgccg acaaggcaac
     3901 ggaagcggtc gacgccgcgc gtgagcgcat cgaagaaatc gtcagcgagc agatcgaccg
     3961 ccaggtcgcg cgccgcacgc gcgcctatcg cgagcaaagc agcgactgga tcatgacatt
     4021 catcatgctg atgattgtca ccatgatcat cgtgaaaatt gtccttgggt ccaagcgcaa
     4081 ggccgaaacg cgtgcccagg cggcgactgc gacggcggcc gaggaaagcc tgaagcgaca
     4141 gcttgccgag gcacagttga agatgatgca ggcacaggtc gagccgcatt tcctgttcaa
     4201 taccctcgca tcggtggatt acctgatcga aacggatccg gcgcgtgctt cgacgatgca
     4261 gaagaacctg atccagtacc tgcgcgcggc gctaccgcag atgcgcgaag gctcgacgac
     4321 gcttggcaag gagatcgcgc tgtgccgctc gtacctcgag atcctgaagg tgcggatgga
     4381 ggatcggttg cagtacgcga tcacggtgcc gcaggggctt gcctcggcgc agttcccgcc
     4441 gatgatgctg caatcgctgg tcgagaattc gatcaagcat ggtctcgagc cgaaggcgga
     4501 gggcggcacg ctcacgatca gcgcggatgt cgcgaacggc aacctgcgcg tgaccgtcgc
     4561 ggacacgggc ctgggtttcg cggcgggcgg gaggccgggc acgggcgttg gattgtcgaa
     4621 cgtgcaggag cgccttgcgg cgctgtacaa cggcgcggcc aagctggtca tcgaagcgaa
     4681 cacgccatcg ggcacgatct cgacgatcga agtgccctac acgatcgatg tcgccagcga
     4741 gccaagtccc gccgcaccgg cagcgcagcc agcgtgagga gggtggcggt ccttagaatg
     4801 gcggcatgac ccgcgcgatc attgccgacg acgaacgcct gatgcgcgaa caactgcgtt
     4861 cgcgactggc cgaggtctgg ccggaactgc agatcgtggc cgaggccagg aatgggcgcg
     4921 aggcggtcga tgtcgtacgc gagtacggtc ccgacctggc attcctcgac atccgcatgc
     4981 ctggaatgac cggtgtcgag gccgcgcgcg aaatagggga tgcgtgtcat atcgtcttca
     5041 tcaccgccta cagcgaatac gcggtcgaag cgttcgagca gggagcggtc gactacgttc
     5101 tgaagccggc cgatcgcgat cgactcgagg taacggtcgc gcgattgcgt aagcgcctcg
     5161 gcgagaagcc ggccgacttg agcgcgctcc tcaacaagct gacattgcgg ctcgatggga
     5221 ttgccgcccc cgcttcagca tcggcagccg gcgaatcgaa acatctgcaa tggattcagg
     5281 catcgatggg tcagcaactg cgcatgatcc cggtggccga cgttctcttc ttcacttccg
     5341 acgaaaaata cacgcgcgtt cagaccgagt cgttcgaggc gctgatccgc aagcccatca
     5401 aggaactcgt cgaggagctg gatccggcgc agttctggca gattcatcgg tcgacgatcg
     5461 tcaacgtgaa ggccattgcg ggcatcacgc gggatttccg ggggcgtcag ctcgtgcagt
     5521 tgaggaacca ctccgaaagg ctcgaggtca gccgcaatta cacccatctg ttcaagcaga
     5581 tgtagcgaaa cgctgagcct caactggttc tgcgcgggaa atgtgagtgc gcgctcacaa
     5641 atgtattccc gcgggtcacc gatcgagcga cgaatcgcgt ccgtccgact cgatccgtgc
     5701 gattgggttt ccccgccttt cgctgcccgg atcggcgtag gctcgtttga caagcctttg
     5761 tcgcgcaacc ataatttgcg gttccctcac ggaggggtgc ccgagcggtt aaagggggca
     5821 gactgtaaat ctgttggctt acgcctacgt tggttcgaat ccaacctcct ccaccaggca
     5881 actacgaagg aaatccggtt aatccagttt ttcgtgcggc gggcgcagcg gtttcgtggc
     5941 tttcgcgcgt cgactcggca ggcgggtgta gctcaatggt agagcagaag ccttccaagc
     6001 ttacgacgag ggttcgattc ccttcacccg ctccagaact ttctccgaag gtgcgctggt
     6061 tgcgcctcgg cccatgtggc tcagtggtag agcactccct tggtaaggga gaggtcacgc
     6121 gttcgatccg cgtcatgggc accagtattt ctgtactttc atcaaccggc cgggtgaaga
     6181 gaccggcgac gaagtccaag cagtcaggag tccgtgatgg ccaaaggcaa atttgagcgc
     6241 acgaagccgc atgtgaatgt ggggacgatt gggcatgtgg atcatggcaa gacgacgttg
     6301 acggcggcca tgacgctggt gttgagcaag aagtatggtg ggg
//
LOCUS       DBNHBY010000496         2310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_371108, whole genome shotgun sequence.
ACCESSION   DBNHBY010000496 DBNHBY010000000
VERSION     DBNHBY010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_371108"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC16HB_12225"
     CDS             <1..422
                     /locus_tag="AC16HB_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR5607598.1"
                     /translation="EGKAREAGLCLKTLGRESGTGSCFGWQVGKCRGACIGKEPRALH
                     DARLQMQLATLRLKPWPFPGPVGIREPAPDARGHCVHVLDHWQHLGTARDESEIEQLL
                     RQGTPAVFDADSYHIIGRALRDVRPRDLIVLSRGGPV"
     gene            422..>2310
                     /gene="rlmKL"
                     /locus_tag="AC16HB_12230"
     CDS             422..>2310
                     /gene="rlmKL"
                     /locus_tag="AC16HB_12230"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HIR5607599.1"
                     /translation="MPAEPSNAALTFFAACPRNVSDLLAAELRERGIAVEREHPAGVS
                     FNGTLRDAYAACLHSRTASRVLLTLAELDASDPDRMYAGLRELPWETHVAPTGTFAVD
                     VVGEPPRWLRHTQFAALRAKDAIVDRMRDIAGERPSVDLAAPDLRVSLRFARERVTVG
                     IDLSGEPLHRRGYRQAGVEAPLKENLAAALLLRAGWPALAEAGGAFRDPMCGSGTLVI
                     EAALIAARVAPGLLRKRFGFERWLQHDAAAWASLREAAVAERALDALPPGRCAGSDRD
                     QVAVRASIANANRAGLGDHLVFERRDLSNLAATTQPQGLVLTNPPYGVRIGDPEKIES
                     LYATLGDKLLRCFPGWEAGVFTGDPPLGRALGLRAYRTHTFYNGAIECRLLRFHLDES
                     AREPDPAEVRAARLEAARARPGAKMFANRLRKNHDRLAQWARREEVACYRVYDADMPE
                     YAFAIDVYGNDERWACVQEYAAPATVAQEAVRARRDEALAMIPEVLQLPSERIVLRVR
                     RRQREGEQYEKLAGEAQFHAVREGRYRFLVNFTDYLDTGLFLDHRLTRSRLGEMARGG
                     RFLNLFAYTGAATVHAVGGGASASTTVDMSRTYLDWARRNLELNGLRAPQHEFVQADC
                     LAWLE"
BASE COUNT          318 a          801 c          841 g          350 t
ORIGIN      
        1 tcgaaggcaa ggcccgcgag gcggggctgt gcctgaagac gctcggccgc gaatcaggta
       61 ccggcagttg cttcggctgg caggtgggca agtgccgcgg cgcgtgcatc ggcaaggagc
      121 cgcgtgcgct gcacgatgcg cgcctgcaga tgcagctcgc gacgctgcga ctgaagccgt
      181 ggccgttccc gggcccggtc ggcatccgcg agcccgcacc ggatgcgcgc ggtcactgcg
      241 tgcacgtgct cgaccattgg cagcacctcg gcacggcgcg cgacgaatcg gagatcgagc
      301 agctcctgcg ccagggaacg cccgcggtct tcgatgctga cagttatcac atcatcggcc
      361 gcgccttgcg cgacgtacgt ccgcgcgacc tgatcgttct ctcgcgcggc ggacccgtct
      421 gatgccggcg gaaccctcga atgcagcgct gacgttcttt gcggcctgtc cgcgcaacgt
      481 cagcgacctg cttgcggccg aattgcgcga gcgcggcatt gccgtcgagc gcgagcatcc
      541 cgccggcgtg agcttcaacg gcacgttgcg cgacgcatac gcggcgtgcc tgcactcgcg
      601 cacggccagc cgcgtgctgt tgacgctcgc tgagctcgat gcgtccgacc ccgatcggat
      661 gtacgccggc ctgcgcgagc tgccctggga gacgcacgtc gcgcccacgg gcacgtttgc
      721 ggtcgacgtg gtgggtgagc cgccgcgctg gctgcggcac acgcagttcg cggcgttgcg
      781 cgccaaggac gccatcgttg accgcatgcg cgacatcgcg ggcgaacgtc cttcggtcga
      841 tctcgccgcg cccgacctgc gcgtcagcct gcgcttcgca cgcgagcgcg tcacggtcgg
      901 catcgacctg agcggcgagc cattgcaccg ccgcggctat cgccaggcgg gggtcgaggc
      961 gccgctcaag gagaacctcg ccgccgccct gctgctgcgc gccgggtggc ccgcgctggc
     1021 cgaggcgggc ggcgcttttc gcgacccgat gtgcggctct ggcacgctcg tcatcgaggc
     1081 ggcgctgatt gcggcacgtg tggcgccggg attgctgcgc aagcgcttcg ggttcgagcg
     1141 ctggctgcag cacgatgccg cggcgtgggc gtcgctgcgg gaggctgccg tcgccgagcg
     1201 cgcgctcgac gcgctgccgc ccggacgctg cgccgggagc gatcgcgacc aggtggcggt
     1261 ccgcgcctcg atcgccaacg ccaatcgcgc gggactcggt gatcacctcg tgttcgagcg
     1321 gcgcgacctg tcgaacctgg ccgcgaccac gcagccgcag gggctcgtgc tgaccaaccc
     1381 gccgtacggc gtgcgcatcg gcgatccgga gaagatcgag tcgctgtacg cgacgctcgg
     1441 cgacaagctg ctgcgctgct ttccgggctg ggaagccggc gtgttcaccg gcgatccgcc
     1501 gctcggacgt gcgctcggcc tgcgcgccta ccgcacgcac acgttctaca acggcgccat
     1561 cgagtgccgc ctgctgcgct tccacctcga cgagtcggcg cgggagcccg atccggccga
     1621 ggtgcgcgcg gcgcgcctcg aagcggcacg cgctcgtccg ggcgcgaaga tgttcgcgaa
     1681 ccggctgcgc aagaatcacg atcggctggc gcaatgggca cgtcgcgagg aggtggcctg
     1741 ctatcgcgtc tacgacgcgg acatgcccga gtacgccttc gcgatcgacg tctacggcaa
     1801 cgacgagcgc tgggcctgcg tgcaggaata cgcggcgccg gcgaccgtgg cgcaggaagc
     1861 cgtgcgcgcg cgtcgcgacg aggcgctggc gatgatcccc gaggtgctgc agttgccgtc
     1921 ggagcggatc gtgctgcgcg tgcggcgccg gcagcgcgag ggtgagcagt acgagaagct
     1981 ggcgggcgag gcgcagttcc acgcggtgcg cgagggacgc tatcggttcc tggtcaactt
     2041 caccgattac ctcgacaccg gactgttcct ggatcaccgg ctcactcgct cgcgcctcgg
     2101 tgagatggcg cgcggtgggc ggttccttaa cctgttcgct tacacgggcg cggccaccgt
     2161 gcacgcggtg ggcggcggcg cgtcggccag caccacggtc gacatgtcgc gcacctatct
     2221 ggactgggcg cggcgcaact tggaactgaa cggcctgcgc gcaccgcagc acgaattcgt
     2281 gcaggccgat tgcctggcgt ggctcgagga
//
LOCUS       DBNHBY010000497         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1047028, whole genome shotgun sequence.
ACCESSION   DBNHBY010000497 DBNHBY010000000
VERSION     DBNHBY010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1047028"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16HB_12235"
     CDS             <1..609
                     /locus_tag="AC16HB_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607600.1"
                     /translation="AVKHGMPAGVAALIVNLQPVLTAVAGRFVGERVNARQWLGLLLG
                     FTGVALVVWSKVTTTGMGFGVVLLAVAALFSMTVATLYQKRFCPHFDLRTGQVIQFVA
                     SVIVTLPFAWATESFHIEWNTAVAAAMLWSIVVLTGGGISLLFLMIRHGATTQVTGYM
                     YLVPAVTALMAWAMFGERLAPTAIAGMVVTIVGVALVVRQRR"
     gene            620..1057
                     /locus_tag="AC16HB_12240"
     CDS             620..1057
                     /locus_tag="AC16HB_12240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5607601.1"
                     /translation="MSERAQPNARGSYRHFLSIPTRWMDNDAYGHVNNVVYYSWFDTV
                     VNEYLIRGGVLDIARGEVIGLVVETQCHYFSELTFPETITAGLRVARIGNSSVRYEIG
                     IFGAADNEASAQGHFVHVYVDRKTRRPAPLPAELRHALERIRA"
     gene            1097..2179
                     /locus_tag="AC16HB_12245"
     CDS             1097..2179
                     /locus_tag="AC16HB_12245"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR5607602.1"
                     /translation="MAKHRIAVIPGDGIGKEVIPEGLRAIDAAARRFGIELAYDHFDF
                     ASCDYYVEHGAMLPDDWKDRIGGHDAIFYGAVGWPATVPDHVSLWGSLLKFRREFDQY
                     VNLRPCRLMPGIPSPLANRKPGEIDFVVVRENTEGEYSSIGGRMFEGTDREVVIQETV
                     MTRVGIDRVLRFAFELAARRPRRHLTSATKSNGISITMPYWDERVAVMAQAFPGIRVD
                     KYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPDRRFPS
                     LFEPVHGSAPDIAGKGIANPIGQIWSGGLMLDWLGYPEAAAAMLRAIEGVLEAGPKNA
                     PLTRDIGGKASTEDLGRAIAEAIARA"
BASE COUNT          434 a          705 c          829 g          471 t
ORIGIN      
        1 gctgtcaagc acggcatgcc ggcgggagtt gcggcgctga ttgtcaacct gcaacccgtg
       61 ctgaccgccg tggcgggtcg cttcgtcggc gagcgcgtca acgcgcggca gtggctgggt
      121 ctgctgctcg gattcacggg cgtcgcgctc gtggtctgga gcaaggtaac gacgaccggg
      181 atgggcttcg gggtcgtgct gctggcggtt gccgcgctct tctcgatgac ggttgccacc
      241 ttgtatcaga agcggttctg cccgcatttc gatctgcgca ccgggcaggt gattcagttc
      301 gtggccagcg tgatcgtgac gctgccgttc gcgtgggcaa ccgagtcgtt tcacatcgaa
      361 tggaacacgg cggtggcggc cgcgatgctg tggtcgatcg tcgtgctcac cggcggcggc
      421 atttccttgc tgttcctgat gatccggcac ggtgctacga cccaagtgac cggttacatg
      481 tatctggttc cggcggtgac ggcattgatg gcatgggcga tgttcgggga gcggcttgcg
      541 ccaacggcaa ttgccggaat ggttgttacc atcgtcggcg ttgccctcgt cgtgcggcaa
      601 cgcaggtgag gagaaacgcg tgagcgaacg tgcgcaaccg aatgcgcgtg gcagttaccg
      661 ccacttcctg tcgataccga cacgctggat ggacaacgac gcctacgggc atgtgaacaa
      721 cgtcgtctat tacagctggt tcgacaccgt cgtcaacgaa tacctgatcc gcggcggcgt
      781 gctcgacatc gcgcgtggcg aagtcattgg actcgtggtc gagacgcagt gccactactt
      841 cagcgagctg acgtttccgg agacgatcac ggccggcttg cgggtggcgc gcattggcaa
      901 ttcgagcgtg cgttacgaga tcggcatctt cggtgccgcc gacaacgagg ccagcgcgca
      961 gggtcatttc gtgcacgtct atgtcgaccg caagacgcgg cgaccggcgc cattgccggc
     1021 ggaactgcgg catgcgctcg agcggatccg tgcctgagcg gacagcgttc aacctttgaa
     1081 ttgaggtgag gcaagaatgg cgaagcatcg gatcgccgtc attccgggcg acggaatcgg
     1141 caaggaggtc ataccggagg ggctgcgcgc catcgacgcg gcggcacgtc gctttggcat
     1201 cgaactggcg tacgaccatt tcgactttgc gtcgtgcgat tactacgtcg agcacggcgc
     1261 gatgttgccg gacgactgga aggaccggat cggcggccac gacgcgatct tctatggagc
     1321 cgttggctgg ccggcaaccg tgcccgatca cgtctcgctg tggggttcgc ttttgaagtt
     1381 ccggcgcgag ttcgaccagt acgtgaacct gcgcccgtgc cgcctgatgc caggaattcc
     1441 atcgccgctg gcaaaccgca agccgggcga gatcgatttc gtcgtcgtac gcgaaaacac
     1501 cgaaggcgag tacagctcga tcggtggacg catgttcgag gggacggacc gtgaggtcgt
     1561 gatccaggag acggtgatga cgcgcgttgg catcgatcgc gtcctcaggt tcgccttcga
     1621 gcttgccgct aggcggccgc gcaggcacct gacttcggcg accaagagca acgggatctc
     1681 gataacgatg ccttattggg acgagcgcgt cgcggttatg gcgcaggcct tccccgggat
     1741 ccgcgtcgac aagtaccaca ttgatatcct gaccgcccac ttcgtccaac accccgactg
     1801 gttcgacgtc gtcgtcgcga gcaatctgtt cggcgacatc ctgtcggatc tgggtcccgc
     1861 gtgcaccgga acgatcggca ttgccccttc ggcaaacctg aatccggacc ggaggttccc
     1921 gtcccttttc gagccggttc acggatcagc gccggatatt gctggcaagg gcatcgccaa
     1981 tcccattggt cagatctgga gcggcggact gatgctggac tggctcggct atccggaagc
     2041 ggccgcggcg atgctgcgcg caatcgaagg ggtgctcgaa gcgggaccga agaatgcgcc
     2101 gctgacgcgc gacattggtg gcaaggcttc gacggaggac ctgggccggg cgatcgcaga
     2161 ggccatcgcg cgggcgtgat ggaccgcgca tcgggcggcg atggagaaaa cgctggttcc
     2221 aagcgcgact ttgtttgtag aatcagcgcg ctgcggccgg gacttcaagc aaaggacaag
     2281 tatttgggcc tgtcggagcg acgtcggatg cgcgaaccgg gcctgcgccg ccgattgcat
     2341 tagtccaaag aatggccgtg ccgggatttt cccgagcatg gaaatccgcg ataagtacgc
     2401 gtgtatcgcg ctttgagggt aggaaatgag catgcgcag
//
LOCUS       DBNHBY010000498         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_167294, whole genome shotgun sequence.
ACCESSION   DBNHBY010000498 DBNHBY010000000
VERSION     DBNHBY010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_167294"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1846)
                     /gene="purL"
                     /locus_tag="AC16HB_12250"
                     /gene_synonym="purI"
     CDS             complement(<1..>1846)
                     /gene="purL"
                     /locus_tag="AC16HB_12250"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR5607603.1"
                     /translation="DADPRGGAQWLVVPRIGTISPWASKATDIARNCGLTSVLRVERG
                     TLFTLVMRNGLFGPKTLDDGAHERIAGLLHDRMTESVLAPDTDPAALFRELPGKPLVR
                     VPLLAEGRAALAGANRDMGLALSADEIDYLLDAYTTLARDPTDVELMMFAQANSEHCR
                     HKIFNANWTIDGRKKEASLFGMIRATHAAAPHGTVVAYSDNAAVIEGRVARRMYPRSN
                     HPRGAVGTEYGARDELTHTVFKVETHNHPTAISPFPGAATGAGGEIRDEGATGRGAKP
                     KAGLCGFSVSHLRLPDARRPWESDRDVAGGEATEADYGFPARIADALSIMIEGPIGAA
                     SFNNEFGRPNLLGYFRAFEANVGGKRYGYHKPIMIAGGIGNIRDDQSGKRDLPHGTLL
                     IQLGGPGMRIGLGGGAASSMGAGANTEQLDFDSVQRGNAEIQRRAQEVIDRCWALGPG
                     NPILSIHDVGAGGLSNALPELADSAKKGARIDLARIPVEESGMSPLETWCNEAQERYA
                     LAIAPEALAAFDWLCKRERCPYAVVGTIDGDDRLVVARNGDAAVDMPMSVLLGKPPKM
                     HRAVEHLPRALPALDLVGLDIERVTLDVLRHPTLASKSFLITIGDRSVG"
BASE COUNT          256 a          652 c          630 g          308 t
ORIGIN      
        1 ccgacactgc ggtccccgat cgtgatcagg aagctcttgc ttgccaaggt cgggtggcgc
       61 agcacgtcga gcgtgacgcg ttcgatgtcc aggccgacga ggtcgagcgc gggcagcgcg
      121 cgcggcagat gctcgactgc gcgatgcatc ttcggcggtt tgccgagcag tacgctcatc
      181 ggcatgtcga ccgcggcatc gccgttgcgc gcgacgacca gtcggtcgtc accgtcgatc
      241 gtgccgacga cggcgtacgg gcagcgctcg cgcttgcaca gccagtcgaa cgccgccagc
      301 gcttcgggcg cgatcgccag ggcatagcgc tcctgcgctt cgttgcacca ggtctccagc
      361 gggctcatcc cgctttcctc gaccgggatc ctggcgagat cgatgcgcgc gcccttcttc
      421 gcgctgtccg cgagttccgg cagcgcattc gacaggccgc ccgcgccgac atcgtggatc
      481 gacaggatcg ggttacccgg gcccaaggcc cagcagcggt cgatgacctc ctgcgcccgg
      541 cgctggatct cggcgttgcc gcgctgaacc gagtcgaagt cgagctgctc ggtgttcgcg
      601 cccgcaccca tgctcgaggc ggcgccaccg cccagcccga tccgcatgcc ggggccgccg
      661 agctgtatca gcagcgtgcc gtgcggcagg tcgcgcttgc ccgactgatc gtcgcggatg
      721 ttgccgatgc cgccggcgat catgatcggc ttgtgatagc catagcgctt gccgccgaca
      781 ttggcttcga acgcgcggaa gtagccgagc aggttgggcc ggccgaattc gttgttgaac
      841 gacgcggcgc cgatggggcc ttcgatcatg atcgacagcg catcggcgat gcgcgcggga
      901 aagccgtagt cggcctcggt cgcctcgccg ccggccacat cgcggtcgct ttcccacggc
      961 cggcgcgcgt cgggcaggcg caggtgcgaa acggaaaatc cgcacaaacc cgccttcggc
     1021 ttcgcgccgc gccccgtcgc gccctcgtcg cggatctcgc cgccggcgcc ggtcgccgcg
     1081 ccgggaaagg gcgagatcgc ggtcgggtgg ttgtgcgttt ccaccttgaa caccgtgtgc
     1141 gtcagctcgt cgcgcgcgcc gtattcggtt cccacagcac cgcgcggatg gttgctgcgc
     1201 ggatacatgc ggcgcgcaac gcgcccttcg atcaccgccg cgttgtcgct gtacgcgacc
     1261 accgtgccgt gcggcgcggc ggcgtgcgtg gcgcggatca tgccgaacag cgacgcctcc
     1321 ttcttccgcc cgtcgatggt ccagttggcg ttgaagatct tgtgccggca atgctccgag
     1381 ttggcctgcg cgaacatcat cagctcgacg tcggtcgggt cgcgcgcgag tgtcgtgtag
     1441 gcgtcgagca ggtagtcgat ctcatcggcg gacagcgcca accccatgtc gcggttcgcg
     1501 cctgcgagtg ctgcgcgccc ctcggccagc aacggcacgc gcacgagcgg cttgccgggc
     1561 agttcacgga acagcgcggc cggatcggta tccggcgcga gcacgctctc ggtcatccgg
     1621 tcgtgcagca acccggcgat ccgctcgtgc gcgccgtcgt ccagcgtctt gggaccgaac
     1681 aggccgttcc tcatcacgag cgtgaacagc gtgccgcgct cgacacgcag cacgctcgtc
     1741 agcccgcagt tgcgcgcgat gtcggtcgcc ttgctggccc acggcgagat cgtgccgatg
     1801 cgcggcacga ccagccattg cgctccgccc cggggatcgg catccg
//
LOCUS       DBNHBY010000499         2599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_32074, whole genome shotgun sequence.
ACCESSION   DBNHBY010000499 DBNHBY010000000
VERSION     DBNHBY010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_32074"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC16HB_12255"
     CDS             complement(<1..449)
                     /locus_tag="AC16HB_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5607604.1"
                     /translation="MQMIRWLTSVCAAALLAACASTPLTEKPTTPTPTPSTAPAAPAP
                     AAAPRTVAPVSAPTLDPLQDPNSPLAKRSVSFDFDEFDVKPEFIGMLEAHGRYLAEHK
                     SQRITIEGNADERGSREYNLALGQRRADAVRARLQLLGASSAQIETI"
     gene            617..>2599
                     /locus_tag="AC16HB_12260"
     CDS             617..>2599
                     /locus_tag="AC16HB_12260"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5607605.1"
                     /translation="MSIRTLDFLLAPRSVAVIGGSDTPLSIGATVMRNLLAGGFAGPI
                     FPVNRKHREVAGRQAWSHVRDLPQTPDLAVICTPAATVPGLIAELGAAGTKAALVLSA
                     GLTARDRGDRSLTQQMLEAARPHLLRVLGPNCVGLLVPGIGLNASFAHIGAQAGKLAF
                     VSQSGALVTAMLDWAGSRGIGFSHFISVGEMADVDFGDLIDYLGGDPGTRAILLYVES
                     ITSARKFLSAARAASRNKPVLIVKSGRAAEGARAAATHTGALAGDDDVVDAAVRRAGM
                     LRVENLLDLFVAVETLARAPRFSGDRLIIVTNGGGAGVLAADAAALGGARVAELSPAT
                     LAKLDAFLPPMWSHGNPVDIIGDAPVERYVEALQVLTDCNDADAVLLIHAPSAIVPAE
                     AIAAACVPIAQKAARPVLSCWLGGQAVESAWRTFTEAGIASYRTPEEAVRAFVQSVTY
                     RRNQEQLMEAPPSRDDANAPDVETARAPLRAALAAKHELLSDPQAKQLLSAYGIAVVD
                     TREVDSTDAAVTAAETIGYPVALKILSPDIAHKTDVGGVALDIGSADELRTEAAMMMK
                     RVLRLQPQARLAGFTVQAMVRRPRAHELIVGVATDPLFGPVIVFGHGGTAVEVVSDRA
                     VALPPLNEALAKEVVSRTRIARLLAGYRDRPAADLAA"
BASE COUNT          358 a          893 c          948 g          400 t
ORIGIN      
        1 ctgatcgtct cgatctgcgc actcgacgcg ccgagcaact gcaggcgcgc cctgaccgcg
       61 tcggcacggc gctggccgag cgcgaggttg tactcgcggc tgccgcgctc atcggcattg
      121 ccttcgatcg tgatccgctg cgacttgtgc tcggccagat acctgccgtg cgcttccagc
      181 atgccgatga actccggctt gacgtcgaac tcgtcgaagt cgaaggagac gctgcgcttg
      241 gcaagcggac tgttcggatc ctgcagcgga tcgagtgtcg gcgcgctgac gggcgccacc
      301 gtccgggggg ccgcggccgg cgccggcgcc gccggggcgg tgctgggcgt cggtgtcggc
      361 gtcgtcggtt tctccgtcag cggcgtgctg gcgcaggccg cgagcagcgc ggccgcgcaa
      421 acggaagtca gccaacgaat catctgcata tcctggttcc ttgcactggg gtccgccaat
      481 ttaggacgat cggccccgcc ttcgtcatgg gtccgcgccg taagggatac ccgtaccgag
      541 cggtccaccg ggtcggcgag gtgacgacag tcaagtgccg cgcgaacgcg tctgccaaca
      601 tcccggcgag acttcgatga gcatccgcac tctcgacttc ctgctggcgc cacggtcggt
      661 cgcggtgatc ggcggctcgg atacgcctct gagcatcggc gcgaccgtga tgcgcaacct
      721 gctcgcgggc ggctttgcgg ggccgatctt cccggtcaac cggaagcatc gcgaagtcgc
      781 gggcaggcag gcctggtcgc acgtgcggga cctgccgcag acgcccgatc tcgcggtgat
      841 ctgcacgccg gccgcgaccg tgcccgggct gatcgccgaa ctgggtgcgg ccggcaccaa
      901 ggcggcgctc gtgctcagcg cgggcctcac ggccagggat cggggcgacc gcagcctgac
      961 gcagcagatg ctcgaggcgg cgcgcccgca cctgctgcgc gtgctcggcc ccaactgcgt
     1021 cgggctgctg gtaccaggca tcgggctgaa cgcgagcttc gcgcacatcg gcgcgcaagc
     1081 cggcaagctc gccttcgtgt cgcagtcggg cgcactggtg accgcgatgc tcgactgggc
     1141 cggttcgcgc ggcatcggtt tctcgcactt catctcggtc ggcgagatgg ccgacgtgga
     1201 cttcggcgac ctgatcgact atctcggcgg cgatcccggc acgcgcgcga tcctgctcta
     1261 tgtcgaatcg atcacgtcgg cgcgcaagtt cctgtcggct gcgcgcgcag cgtcgcgcaa
     1321 caagccggtg ctgatcgtca agagcggacg cgcggccgaa ggagcgcgcg ccgcggcgac
     1381 gcacacgggc gcgctcgccg gcgacgacga tgtggtcgac gctgccgtgc gccgcgcggg
     1441 gatgctgcgc gtcgagaatc tgctggacct gttcgtcgcg gtcgagacac tggcgcgcgc
     1501 gccgcgcttt tccggcgacc ggctgatcat cgtcaccaat ggcggcggcg ccggcgtgct
     1561 ggcggccgat gccgccgcgc tcggcggtgc gcgtgtggcc gaactgtcgc ccgccacgct
     1621 ggcgaagctg gacgcgttcc tgccgccgat gtggtcgcac ggcaacccgg tggacatcat
     1681 cggcgacgct ccggtcgagc gctacgtgga ggcgctgcaa gtgctgaccg actgcaacga
     1741 cgccgacgcg gtgctgctca tccacgcgcc gtcggcgatc gtgcccgccg aggcgatcgc
     1801 ggccgcgtgc gtgccgatcg cgcagaaggc ggcgcggccg gtcctgtcgt gctggctcgg
     1861 cgggcaggcg gtggagagtg catggcgcac gttcaccgag gcgggcatcg cgagctatcg
     1921 gacgcccgag gaagcggtgc gcgcgttcgt gcagtcggtc acgtaccgcc gcaaccagga
     1981 acagttgatg gaggcgccgc cgtcgcgcga cgacgcgaac gcgcccgacg tcgagaccgc
     2041 gcgagcgccg ctgcgggccg cgctcgcggc caagcatgaa ctgctgtccg atccgcaggc
     2101 caagcaactg ctgtcggctt acggcatcgc ggtggtcgac acgcgcgaag tcgattcgac
     2161 tgacgcggcg gtcacggcgg ccgagaccat cggttatccg gtggcactga agatcctgtc
     2221 gcccgacatc gcccacaaga ccgacgtcgg cggcgtggcg ctcgacatcg gctcggccga
     2281 tgaactgcgc accgaagcgg cgatgatgat gaagcgcgtg ctgcggctgc agccgcaggc
     2341 gcgcctggcc ggcttcacgg tgcaggcaat ggtgcgccgg ccgcgcgccc acgagctgat
     2401 cgtcggcgtg gccaccgatc cgctgttcgg cccggtgatc gtgttcggtc acggcggcac
     2461 cgcggtcgaa gtcgtgtccg accgcgcggt cgcgctgccg ccgctgaacg aggcgctggc
     2521 gaaggaggtc gtttcgcgca cgcgcatcgc gcggctgctc gcgggctatc gcgaccgtcc
     2581 ggcggccgat cttgcggcg
//
LOCUS       DBNHBY010000500         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1521544, whole genome shotgun sequence.
ACCESSION   DBNHBY010000500 DBNHBY010000000
VERSION     DBNHBY010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1521544"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="AC16HB_12265"
     CDS             complement(<1..430)
                     /locus_tag="AC16HB_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607606.1"
                     /translation="MKTLRQSWRTMVALGLPLVAASNLQPAAAQVVDDSACPRYAVDI
                     ASFATCDGDRVAGSEGIERVSPQRAHELKQRRGRAVLLIDVRSKAEVLLSGAPLGIDA
                     VVPLAEIAQPLRWDAEQRDLQLVDDPNFGVLVQASVAALKG"
     gene            complement(446..784)
                     /locus_tag="AC16HB_12270"
     CDS             complement(446..784)
                     /locus_tag="AC16HB_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607607.1"
                     /translation="MNSRYDYLQDPQSRSRLALFRSSVAVGIVIGVLAIGTLAAKGAD
                     PGAAVGTEFSDAATSMYATGLAPEPAPVESPGATDLLVDPARASAAAIEFSGALGRFS
                     DEPAEQPPTF"
     gene            1057..1611
                     /locus_tag="AC16HB_12275"
     CDS             1057..1611
                     /locus_tag="AC16HB_12275"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR5607608.1"
                     /translation="MGDITQLLCRARDGDQAALNALFAELYPELRRLAHARLAGGGRH
                     TLMETSVLIHECYMKFAGAGRIEISDRAHFMAYTARAMRSIIVDYARGRLRERRGGDA
                     AHTSLNTEMIDGLPAGDDQVVSVHEALETLAQREPRLAQVVEMRYYGGLSEQEIAAAL
                     GVTDRTVRRDWEKARLLLADALRA"
BASE COUNT          284 a          577 c          621 g          282 t
ORIGIN      
        1 cgcctttgag cgcggcgacc gacgcttgca cgaggacgcc gaagttcgga tcatcgacca
       61 gttgcaggtc gcgttgctcc gcgtcccagc gcagaggctg cgcgatctcg gccagtggca
      121 ccacggcgtc gatgcccagt ggtgcgccgg acagaagcac ctccgccttc gaccgcacgt
      181 cgatcagcag cacggcgcgt ccgcgccgct gtttgagttc gtgcgcgcgc tgcgggctca
      241 cgcgctcgat gccttcgctt cccgccaccc ggtcgccgtc gcaggtggcg aatgacgcga
      301 tgtccactgc gtaacgcgga catgcactgt cgtcgacgac ttgcgccgcg gccggctgca
      361 ggttcgaggc ggccaccagc ggcagcccca gcgccaccat cgtccgccac gactgtcgga
      421 gtgtcttcat ttcgacctcg gcgactcaga acgtgggcgg ctgctcggcc ggctcatccg
      481 agaagcgacc gagcgcgccg gagaactcga tggccgccgc tgatgcgcgc gcggggtcga
      541 ccaagaggtc ggtcgcaccc ggggattcga ctggtgccgg ctccggagcg agaccggttg
      601 cgtacatcga agtcgcggcg tcgctgaatt cggtgcccac agcggcgccc gggtctgcgc
      661 ccttggcggc cagcgtgccg atggcgagta ctccaatcac gatcccgacg gcgacgctgg
      721 agcgaaagag cgcgagccgg ctgcgcgatt gcggatcctg caggtagtcg tagcgcgagt
      781 tcatggactt gcctcctggt tgatcggacc gcacgattcg gcggcccggc tactttcgaa
      841 tcggggcacc cacggatgcc ccaccgattg cgcgtgaaga ctcacgaacc gcgtaggcgc
      901 gaagctcaga tgccccgcat cgcggtcggc tgccatcaag aacagcccgc ctcatctggg
      961 aacacgacgc cacggggccg aaccggacat ggctatgaag cccttcttcg cgtggagggg
     1021 cgacgggcgc tagcatctgc gccggaaggg gatcgcatgg gcgacatcac gcagctgctt
     1081 tgccgtgcgc gcgacggcga ccaggctgca ctcaatgcgc ttttcgccga gctctatccc
     1141 gaattgcggc ggctggcgca cgcgcggctc gcgggcggcg gtcgccacac gctgatggaa
     1201 acctcggtgc tcattcacga gtgctacatg aagttcgccg gcgcggggcg gatcgagatc
     1261 agcgaccgcg cgcacttcat ggcgtacacg gcgcgcgcga tgcgctcgat catcgtcgac
     1321 tacgcgcggg gccggctgcg cgagcggcgc ggcggagacg ctgctcacac ttcgctgaac
     1381 acggaaatga tcgatggcct gcccgccgga gacgaccagg tagtttcggt gcacgaagcg
     1441 ctggaaacgc tggcgcagcg tgagccgcgt ctcgcgcagg tggtcgagat gcgctactac
     1501 ggcgggctga gcgagcagga gatcgcggcc gcgctcggtg tcacggaccg cacggtacgg
     1561 cgcgactggg aaaaggcacg tctgctgctg gccgatgcat tgcgcgcgta gcactgccct
     1621 ccaatgagcg ctgcgaacga cctcaccgcg tccagttggc gtgtcttcag tgggctgctg
     1681 gacgaggcac tcgagttgcc ggtgggtgag cggttgacgt ggctcgatgg gttgggtgtc
     1741 gagcatgagg cgctgaagcc ggcg
//
LOCUS       DBNHBY010000501         2989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_979937, whole genome shotgun sequence.
ACCESSION   DBNHBY010000501 DBNHBY010000000
VERSION     DBNHBY010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_979937"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..370)
                     /locus_tag="AC16HB_12280"
     CDS             complement(179..370)
                     /locus_tag="AC16HB_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5607609.1"
                     /translation="MRFIVTLFLALIVLTALQPWLQKLGIGRLPGDFRIRIGRREYLL
                     PFASTLLLTVIAFVIGRLI"
     gene            complement(391..1545)
                     /locus_tag="AC16HB_12285"
     CDS             complement(391..1545)
                     /locus_tag="AC16HB_12285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607610.1"
                     /translation="MPHEPRPTLPAPGAEALAHSARVAQHIRAEIERAGGWIGFDRYM
                     ELALYAPGLGYYAAGTRKFGDAASGGDFVTAPEISPPYARALTNQVTQAFEHYSASVL
                     EFGAGSGVLARDLLAALERRGVRDVRYAIVELSADLRERQRATLGGRGVEWLTAAPAR
                     FDGVVIANEVLDVMPVQLFVKRDGVVRERGVSTDDSARLAFAEAPAGRDLAAAVAAIE
                     AEVGALPDGYGSEVGPIARAWVASLADWLARGVALLIDYGFPRREYYHPQRLMGTLMC
                     HYRHHAHADPLWLPGLNDITAHVDFSALADAAHAAGLSVLGYTSQAHFLLNCGLPELL
                     QAERTHARANEAHRLISEAEMGELFKVLALGRGVDAPLLGYVRGDRSHRL"
     gene            1577..2362
                     /locus_tag="AC16HB_12290"
     CDS             1577..2362
                     /locus_tag="AC16HB_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607611.1"
                     /translation="MTKNAAATPRVALVTGAERRIGRAIALALADDGWDIAVHYGTSR
                     EEALRTVGAVEHSGRRAAAIGRDLADEAGVLELLDDCAAALGAVQCVVNNASVFEYDD
                     AARFSGAMLDRMMRINAAAPILLARSLHARVPDGSQGVVVNLLDQKLWNPNPDFLSYT
                     LSKAALREATTLLAQALAPRVRVVGVAPGITLASGTQSDAGFAAAHVTAPLGRGSAPE
                     DVAQAVVYLANARAVTGITLLVDGGQHLAPSTRDVMFLTEPRQ"
     gene            2359..2760
                     /locus_tag="AC16HB_12295"
     CDS             2359..2760
                     /locus_tag="AC16HB_12295"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5607612.1"
                     /translation="MISWWSDPRLADCRRIFLREVLLDANIGIHAFEKAGAQRLAISV
                     DLFVPLELSTPHHDRISEVVDYDFVRTTIRHRIERGHINLQETLVDDLARELLAHPAA
                     VAVRVSSEKPDVYADVEAVGVEVFRFKEAAA"
     gene            2757..>2989
                     /locus_tag="AC16HB_12300"
     CDS             2757..>2989
                     /locus_tag="AC16HB_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR5607613.1"
                     /translation="MNAPTELLRAEKRAREDHKLEKRLCRLAGQAIADFNMIEHGDRV
                     MVCLSGGKDSYGLLDVLLKLRERAPIAFEIVAV"
BASE COUNT          464 a         1053 c         1058 g          414 t
ORIGIN      
        1 cgcgcggcgc gccgtcgtcc ggctcgccgg ccgcggcgct gtcgacccgc cacacggcgc
       61 gccgccgcgc gatcagcggc ctttcgaact cgatgtcgaa cgcgttgtcg gccatgattg
      121 tgctcggcgg ccgcgatgat gccgcgcacg cgcgccgaaa atccgactga tcgcgtgttc
      181 agatgagtcg gccgatcacg aacgcgatca ccgtgagcag cagcgtgctg gcgaacggca
      241 gcaggtactc gcgccggccg atgcggatgc gaaagtcccc cggcagccgg ccgatgccaa
      301 gcttctgcag ccacggctgc agcgcggtca gcacgatcag ggcgaggaaa agcgtgacga
      361 tgaagcgcat cttcggtcgg gcgtcgcgcg ctacagccgg tgcgaacgat cgccgcgcac
      421 gtagccgagc agcggcgcgt cgacgccgcg gccgagcgcg agcaccttga agagttcgcc
      481 catctccgcc tcggagatca ggcggtgcgc ctcgttggcg cgcgcgtgcg tgcgttcggc
      541 ctgcagcagt tcggggaggc cgcagttcag caggaagtgc gcctgcgacg tatagcccag
      601 caccgacagt ccggccgcgt gcgccgcgtc cgccagcgcg ctgaagtcga catgcgcggt
      661 gatgtcgttc agccccggca gccacagcgg atcggcgtgc gcgtggtggc ggtaatggca
      721 catcagcgtg cccatcagcc gctgcggatg gtagtactcg cggcgcggaa aaccgtagtc
      781 gatcagcagc gcgacgccgc gcgcgagcca gtccgcgagc gacgccaccc acgcgcgcgc
      841 gatcgggccg acctccgaac cgtagccgtc gggcagcgca ccgacctcgg cctcgatcgc
      901 ggccaccgcc gccgcgagat cgcgtcccgc gggcgcttct gcgaacgcga ggcgggcgct
      961 atcgtccgtc gaaactccgc gctcgcgcac gacgccgtcg cgcttgacga acaactgcac
     1021 cggcatcacg tcgagcacct cgttggcaat caccacgccg tcgaagcgcg ccggcgcagc
     1081 cgtcagccac tcgacgcccc ggccgccgag ggtggcgcgc tggcgctcgc gcaggtcggc
     1141 cgacagttcg acgatcgcat agcgcacgtc gcgcacgccg cgccgctcga gcgcggcgag
     1201 caggtcgcgt gcgagcacgc cggaaccggc gccgaactcg agcacgctcg ccgaatagtg
     1261 ctcgaacgcc tgcgtcacct ggttcgtgag cgcccgcgcg taaggcggcg agatttccgg
     1321 cgcggtgacg aagtcaccgc cgctggccgc atcgccgaac ttgcgcgtgc ccgcggcgta
     1381 atagccgagt cccggcgcgt acagcgcgag ttccatgtag cgatcgaagc cgatccaccc
     1441 gcccgcgcgc tcgatctcgg cacggatgtg ctgcgccacc cgagcgctgt gcgcgagcgc
     1501 ctccgcgccg ggcgcgggaa gggtgggtcg cggctcatgg ggcatggccg atcctaccgt
     1561 taaacttcgc cgccccatga ccaagaacgc tgcagcgaca cccagggtcg cgctcgtcac
     1621 cggtgcggag cgccgcatcg gccgcgcgat cgcgctggcg ctcgcggacg atggctggga
     1681 catcgcggtc cattacggca cctcgcgcga ggaggcgctg cggacagtcg gcgcggtcga
     1741 gcacagcggc cggcgcgcgg cggcgatcgg ccgcgacctc gccgacgaag ccggggtgct
     1801 cgagttgctc gacgactgcg ccgctgcgct aggcgcggtt cagtgcgtgg tcaacaacgc
     1861 gtcggtcttc gagtacgacg atgcggcgcg cttcagtggc gcgatgctcg accgcatgat
     1921 gcgcatcaac gccgccgcgc cgatcctgct cgcgcgcagc ctgcacgcgc gcgtgcccga
     1981 cggctcccag ggcgtggtcg tcaacctgct cgaccagaag ctgtggaatc cgaatccgga
     2041 cttcctcagc tacacgctgt cgaaggcggc gctgagggag gcgacaacgc tgctggcgca
     2101 ggcgctggcg ccccgggtgc gcgtggtcgg ggtggcgccc ggcatcacgc tggcgtccgg
     2161 cacgcaaagc gatgcggggt tcgccgccgc gcatgtcacc gcgccgctgg ggcgcggcag
     2221 cgcgccggaa gacgtggcgc aggcggtcgt ttacctggcc aatgcgcgcg ccgtgaccgg
     2281 catcacgctg ctggtcgacg gcggccagca tctcgcgccg tcgacgcgcg acgtgatgtt
     2341 cctgacggag ccccggcaat gatctcgtgg tggagcgacc cgcggctggc ggactgccgg
     2401 cgcatcttcc tgcgcgaggt gctgctggac gccaacatcg gcatccacgc gttcgagaag
     2461 gcgggcgcgc agcggctcgc gatcagcgtc gacctgttcg tgccgctcga attgtccacg
     2521 ccacaccacg accgcatcag tgaagtggtc gactacgact tcgtgcgcac gacgatccgc
     2581 caccggatcg agcgtggcca catcaatctg caggaaacgc tggtcgatga cctggcgcgc
     2641 gaactgctcg cgcatcccgc ggccgtcgcg gtgcgcgtgt cgagcgagaa gcccgacgtg
     2701 tacgccgacg tcgaggcggt tggcgtcgag gtgtttcgct tcaaggaggc cgccgcatga
     2761 acgcgcccac ggaactgctg cgcgcggaga agcgtgcccg cgaagaccac aagctggaga
     2821 aacgcctatg tcggctggcc gggcaggcga tcgccgactt caacatgatc gagcacggcg
     2881 accgcgtgat ggtctgcctg tccggcggca aggacagcta cggcctgctc gacgtgctgc
     2941 tcaagctgcg cgagcgcgcg ccgatcgcgt tcgagatcgt cgccgtcaa
//
LOCUS       DBNHBY010000502         7413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_980032, whole genome shotgun sequence.
ACCESSION   DBNHBY010000502 DBNHBY010000000
VERSION     DBNHBY010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_980032"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..175
                     /locus_tag="AC16HB_12305"
     CDS             8..175
                     /locus_tag="AC16HB_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR5607614.1"
                     /translation="MAAHYVGALAATVLGGVWLAGRVWYAFAYMTNPKKRGPGFSLAY
                     VAWGLMMVGAA"
     gene            205..2028
                     /locus_tag="AC16HB_12310"
     CDS             205..2028
                     /locus_tag="AC16HB_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5607615.1"
                     /translation="MAAQATASAATRATATPAPAARRLRPLRSLLPNVTVYRKQIALA
                     LLFLLLAAASTLALPYAVKLLVDGGLALPAGTEVGARLVAIREHFLGLFGVSVALGLF
                     TAGRFYMVTWIGERVTTDLRQAVYAHVLQQSPQFFETLKTGEVLSRLTTDTTVIQSAV
                     GSSISMGLRNTVLFVGGIAMLIATSPRLMLTVTGIIVLVVLPALVIGRRVRKLSRASQ
                     DRIADTSGIAGEILNAIPIVQSFTQERAETERFGSANEQAFAASVRRTGMRASLTAFV
                     IIGVFGSLLWGLYGGVQAVLAGKTTAGELSQTALYVTLVASSVAVLAEVWGDLLRAAG
                     ATERLMELLDARSAIADPAQPRALPARHGGARIEFASVRFAYPSRPARPVLHDLTLAI
                     EPGQTVALVGPSGAGKTTIFQLLQRFYDIDAGAVRFDGVDVRELALADLRSRIAVVPQ
                     EPVIFSGSVLDNIRYGRASATEDEAMAAARAAFVDEFVRQLPEGYATFVGERGLRLSG
                     GQRQRLAIARAMVKDAPLLLLDEATSSLDAESERAVQAALEVAISGRTTIVIAHRLAT
                     VQRADRIFVLEAGRIVEAGTHAELVARAGLYARLAALQFAA"
     gene            complement(2062..2424)
                     /locus_tag="AC16HB_12315"
     CDS             complement(2062..2424)
                     /locus_tag="AC16HB_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607616.1"
                     /translation="MLTIPAARTRLQSPLIAAALAAATLVLGGTAVAQQRDAPASSWL
                     GPDRISLAALEDAFWVCDYIATTRGARDDAITRCGAVYDALKERKFGGDFDRLLAWWQ
                     ENKPAQFARLDHDKHALR"
     gene            2603..3751
                     /locus_tag="AC16HB_12320"
     CDS             2603..3751
                     /locus_tag="AC16HB_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5607617.1"
                     /translation="MRMLICLTALLLTACASAPGPVARPDALLHDELFAAPTERISAD
                     DVFALSEPMRRFLKTEVADEIRWHGYQAGLAAALHEPGKLKLQYDAATTRNAAQAFEA
                     RAGNCLSLVLMTAAFAKELGLPVVYQSAYLEETWNRSGNLLVRSGHINITLGGRLVDA
                     GTTRWLHPLTIDFLPVSEIRGLRVREVDESTVVAMYMNNRAVEALAQGRADDAYGWVR
                     EAIRQDPRLLIAVNTLGVIYLRQGALAAAAEAFGYVTERDPDNTQALANLADVYAKQG
                     RTEQANALLRRLAQLEPYPPFHFFILGQAAMQRGDFRAARDLFAREVARADYNPEFHY
                     WLGLASFRLGEFDAARRQLALARDSSTSRRDRDLYAAKLSWLRSYGAQ"
     gene            3851..5806
                     /locus_tag="AC16HB_12325"
     CDS             3851..5806
                     /locus_tag="AC16HB_12325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5607618.1"
                     /translation="MMPMVRRTAQRWLAPALLGLAALTQLPALAQTTAQPPQFPVRDF
                     FSNPEQAYFRLSEDGRTLGFMQPVSVAGGERRLNVFVQPLAGSRPVGTPRRLTGETAR
                     DISIYYWKGSGTILYEKDFGGDENFHVVAVDVKSGKVTDLTPGDKVRASILDPLPDDP
                     RHILVSHNRRNPEVFDVYRIDLGTGKEALVARNPGDIVGWQTDHAGRVRVAVRTEGLN
                     TILLYRASDKGDFRPIVTTDYRTEVDAAFFTADDRRLYAISNRGRDRKALVVIDPARP
                     DTEQMVFEHPEVDLAGATWSRLRKKLTYAEYQTDRIGRRFFDRMTEGIFKRLAEKLPG
                     MEITLQSATKAEDKFIVAAANDRTPGARYIYDARADTLTKLADINPKIPESAMAPMQP
                     VAYTSRDGLTIHGYLTLPVGRAPKGLACIVNPHGGPWYRDAWGYNPEVQFLANRGFCV
                     LQMNFRGSTGYGRKFWEASFGQWGLKMQDDISDGVKWLVDRGIADPRRIGIYGGSYGG
                     YATLAGVTFTPDLYAAAVDYVGVSNLFTFMNTIPPYWKPLLPKFYDMVGEPQKDKERL
                     AATSPALHADRIRTPLLVVQGARDPRVNKAESDQMVEALRKRGVDVEYIVKDNEGHGF
                     RNEENQFEIYGAMEAFFLRHLKPDASG"
     gene            complement(5890..7158)
                     /locus_tag="AC16HB_12330"
     CDS             complement(5890..7158)
                     /locus_tag="AC16HB_12330"
                     /inference="COORDINATES: protein motif:HMM:NF020322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5607619.1"
                     /translation="MFESLQSAIASNPLLSGGTALAAAGILALWLREVPGKLAAWGQH
                     FFVATLAVDSRDELMFPALVEYMDSRDALRRINNFTVRTLRQQGSSYQSLHDELQQGG
                     RPAALFSPGEGFHVFVLDGRLMWMKREVQVGVAVVEKITLSTFGRGRGALESIVNSAL
                     NARIERELNRIAVYVPSPYSNEWTRARLGNNRRLDSVVLKAGQKEAIRADIERFFASR
                     ARYEALGIPWRRGYLLYGPPGTGKTSLVTALASELSLNVCVLSLASPNVTDEKIGNLL
                     ASVPVRSVILIEDVDAFFQARLKADAGVKVSYSGFINALDGVAAHEGSVVFLTTNHPH
                     LIDEAAIRSGRVDFRLELGLCDRGQLEQMFLKFFDDAAAAARFADAVPAGRWSPSQVQ
                     ERLLKAASANEAIELFVEPPAAVVTQLRAA"
BASE COUNT         1164 a         2608 c         2557 g         1084 t
ORIGIN      
        1 gctgtggctg gcggcgcact acgtcggcgc cctggcggcg accgtgctcg gcggcgtgtg
       61 gctggccggc cgcgtctggt acgcattcgc atacatgacc aacccgaaga agcgcggccc
      121 gggattctcg ctcgcctacg tcgcgtgggg actgatgatg gtcggcgccg cgtagggcgt
      181 gctcggcaat ctcgcgggca agtgatggca gcgcaggcaa ccgcatccgc cgcgacccgg
      241 gcgaccgcaa cgccggcacc tgcggcgcgc cggttgcgcc cgctgcgctc cctgctgccg
      301 aacgtgacgg tctaccgcaa gcagatcgcg cttgcgctgc tcttcctgct gctcgcggcg
      361 gcgagcacgc tggcgctgcc ctatgcggtc aagctgctgg tcgacggcgg gctcgcgctg
      421 ccggccggca ccgaggtcgg cgcgcggctc gtggcgatcc gcgagcactt cctgggcctg
      481 ttcggcgtgt cggtcgcgct cggcctcttc accgcgggac gcttctacat ggtcacgtgg
      541 atcggcgagc gcgtgacgac cgacctccgc caggccgtct acgcgcacgt gctgcagcag
      601 agcccgcagt tcttcgagac gctgaagacg ggcgaagtgc tgtcgcgcct gaccaccgac
      661 acgacggtga tccagagcgc ggtcggctcg agcatctcga tggggctgcg caacacggtg
      721 ctgttcgtgg gcgggatcgc gatgctgatc gccaccagcc cgcggctgat gctgaccgtg
      781 accggcatca tcgtgctggt cgtgctgccg gcgctcgtca tcggccgccg cgtgcgcaag
      841 ctgtcgcgcg caagccagga ccgcatcgcc gacaccagcg gcatcgccgg cgagatcctg
      901 aacgcgatcc cgatcgtgca gagcttcacg caggagcgcg cggagacgga gcgcttcggg
      961 tccgccaacg agcaggcctt cgcggccagc gtgcgccgca ccggcatgcg cgcgtcgctg
     1021 accgcgttcg tgatcatcgg cgtgttcggc tcgctgctgt ggggactgta cggtggcgtg
     1081 caagcggtgc tcgccggcaa gaccacggcg ggcgaactgt cacagacggc gctgtacgtc
     1141 acgctggtcg cctccagcgt ggcggtgctg gccgaggtgt ggggcgacct gctgcgcgcc
     1201 gccggcgcga ccgagcggct gatggaactc ttggatgcgc gctccgcgat cgcggatccg
     1261 gcgcagccgc gtgcgctgcc agcgcgacac ggcggcgcgc gcatcgagtt cgcctcggtg
     1321 cgcttcgcct atccgtcgcg gccggcgcgg cccgtgctgc acgatctcac gctcgcgatc
     1381 gagccggggc agacggtcgc gctggtcggc ccgtcgggcg cgggaaagac gacgatcttc
     1441 cagctgctgc agcggttcta cgacatcgac gccggtgccg tgcgcttcga cggcgtcgac
     1501 gtgcgcgaac tcgcgctcgc cgacctgcgc agccgtatcg cggtcgtgcc gcaggagccg
     1561 gtgatcttct ccggcagcgt gctcgacaac atccgctatg ggcgcgccag tgcgaccgaa
     1621 gacgaggcga tggccgcggc gcgcgcggcg ttcgtcgacg aattcgtgcg ccagctaccc
     1681 gaggggtacg cgacgttcgt cggcgagcgc ggattgcgcc tgtccggcgg gcagcggcag
     1741 cggctcgcga tcgcgcgcgc gatggtcaag gatgcaccgt tactgctcct cgacgaggcg
     1801 accagcagcc tcgacgccga gagcgagcgc gcagtgcagg ccgcgctcga ggtcgcgatc
     1861 agcggccgca cgacgatcgt gatcgcgcac cggctggcga ccgtgcagcg cgccgaccgc
     1921 atcttcgtgc tggaggccgg ccgcatcgtc gaagcgggca cgcacgcgga actggtcgca
     1981 cgcgccggcc tttacgcgcg gctggcagcg ctgcagtttg ccgcatgagc agggtcagtc
     2041 aaatgcggcg cgcgtccggc gtcaccggag cgcgtgctta tcgtgatcga gccgcgcgaa
     2101 ctgcgccggc ttgttctctt gccaccatgc gagcagccgg tcgaagtcgc cgccgaactt
     2161 gcgttctttc agcgcgtcgt aaaccgcccc gcaccgggtg atcgcgtcat cccgggcgcc
     2221 gcgcgtggtc gcgatgtaat cgcagaccca gaaggcgtct tcaagtgccg cgaggctgat
     2281 gcggtcggga ccaagccacg acgatgccgg cgcgtcccgc tgctgcgcca ccgcggtgcc
     2341 gcccagcacc agcgtggccg cggccagcgc ggcggctatc aacggggatt gcaggcgggt
     2401 tcgcgctgcc gggatcgtga gcattcgcac gtctccttcg tggtcgacgg atcgctcgcg
     2461 ctcgcggcaa acgcgcgaac cggagtatcg ccagtgcgcg tctgcctgtc tctccctcga
     2521 ccgggtggga tcgccgcccc gggattcggg gcgcggcggc cggcgctaca gttgcgacaa
     2581 gactcggtat cggagcgttc tcatgcgcat gctgatctgc ctgaccgccc tgctcctgac
     2641 cgcctgcgcc agcgcgcccg gccccgtcgc gcgccccgac gccctgctgc acgacgaact
     2701 gttcgcggcg ccgaccgagc gcatcagtgc cgacgacgtg ttcgcgttga gcgaaccaat
     2761 gcgccgcttc ctgaagacgg aggtcgcgga cgagatccgc tggcacggct accaggccgg
     2821 gctcgcggca gcgctgcacg aaccgggcaa gctgaagctg cagtacgacg cggcgaccac
     2881 gcgcaacgcg gcgcaggcgt tcgaggcgcg cgccggcaac tgcctgtcac tggtgctgat
     2941 gacggccgcg ttcgcaaagg aactgggcct gccggtggtc taccagagcg cgtacctgga
     3001 ggagacctgg aaccgcagcg gcaacctgct ggtgcgcagc ggacacatca acatcacgct
     3061 cggcgggcgg ctggtcgacg ccggcaccac gcgctggctg catccgctga cgatcgactt
     3121 cctgccggtg agcgagatcc gcggcctgcg cgtacgcgag gtcgacgagt cgacggtggt
     3181 cgcgatgtac atgaacaacc gcgcagtcga ggcgctggca cagggtcggg ccgacgacgc
     3241 ctacggctgg gtgcgcgagg cgatccgcca ggacccgcgc ctgctgatcg ccgtcaacac
     3301 gctcggcgtg atctacctgc ggcagggcgc gctggcggcg gccgcggagg cgttcgggta
     3361 cgtgaccgag cgcgacccgg acaacacgca ggcgctggcg aacctcgccg atgtttacgc
     3421 gaagcagggc cgcacggagc aggccaacgc gctgctgcgc cggctggcgc aactcgagcc
     3481 gtatccaccg tttcatttct tcatcctcgg ccaggcggca atgcagcgcg gcgacttccg
     3541 cgccgcgcgc gacctgttcg cgcgcgaggt cgcgcgtgcc gactacaacc cggagttcca
     3601 ctactggctc ggactggcga gtttccgcct cggcgagttc gatgcggcgc ggcgccagct
     3661 cgctctggcg cgggacagca gcacgtcgcg ccgcgatcgc gacctgtacg cggccaagct
     3721 ctcctggctg cgcagctacg gcgcgcagta gcacgcaatt cgttccccgc atcggccgct
     3781 tcgtcgcacg gcgctcgccc ggcggttccg acatggtcag actgcgccac cgcttcaaga
     3841 ggagacacag atgatgccaa tggttcgccg gaccgcgcag cgctggctcg cgcccgcgct
     3901 gctcggcctc gcggcgctga cccaactgcc ggcgctggcg cagaccaccg cgcagccgcc
     3961 gcagtttccg gtccgcgatt tcttcagcaa cccggagcag gcgtacttcc gcctcagcga
     4021 agacggccgc acgctcggct tcatgcaacc ggtatcggtc gccggcggcg agcggcggct
     4081 caacgtgttc gtgcagccgc tcgcgggcag ccggccggtc ggcacgccgc gccggctgac
     4141 gggcgagacc gcgcgcgaca tcagcatcta ctactggaag ggcagcggca cgatcctgta
     4201 cgagaaggac ttcggcggcg acgagaactt ccacgtggtc gcggtcgacg tgaagtcggg
     4261 caaggtcacg gatctcacgc cgggcgacaa ggtacgcgcc agcatcctcg atccgctgcc
     4321 cgacgatccg cgccacatcc tggtcagcca caaccgccgc aatccggagg tgttcgacgt
     4381 ctaccggatc gacctcggca cgggcaagga ggcgctggtc gcgcggaacc cgggcgacat
     4441 cgtcggctgg cagaccgatc acgccggccg cgtgcgcgtc gcggtgcgca ccgagggact
     4501 gaacacgatc ctgctctatc gcgccagcga caagggcgac ttcaggccca tcgtcaccac
     4561 cgactacagg accgaggtcg atgcagcgtt cttcaccgcc gacgaccgcc ggctctacgc
     4621 gatcagcaac cgcgggcgcg atcgcaaggc gctcgtcgtc atcgacccgg cacggcccga
     4681 caccgaacag atggtgttcg aacatcccga ggtggacctc gccggcgcca cgtggtcgcg
     4741 gctgcgcaag aagctgacct acgcggaata ccagaccgat cgcatcgggc gcagattctt
     4801 cgaccggatg accgagggca tcttcaagcg cctcgcggaa aagctgccgg gcatggagat
     4861 cacgctgcag agcgcgacca aggccgagga caagttcatc gtcgccgccg ccaacgaccg
     4921 cacgcccggc gcgcgctaca tctacgacgc ccgggccgac acgctgacca agctcgccga
     4981 catcaacccg aagatcccgg aatcggcgat ggcgccgatg cagccggtcg cctacacgag
     5041 ccgcgacggg ctgacgatcc acggctatct gacgctgccg gtcggccgcg cgcccaaggg
     5101 cctcgcgtgc atcgtcaacc cgcatggcgg accgtggtac cgcgacgcct ggggttacaa
     5161 ccccgaagtg cagttcctcg ccaaccgcgg cttctgcgtg ctgcagatga atttccgcgg
     5221 ctcgaccggc tacggccgca agttctggga ggcgagcttc ggccagtggg gcctgaagat
     5281 gcaggacgac atcagcgacg gcgtgaagtg gctggtcgac cgcggcatcg ccgatcccag
     5341 gcgcatcggc atctacggcg ggagctacgg aggatatgca acgctggcgg gcgtgacctt
     5401 cacgcccgac ctctacgcgg ccgcggtcga ctacgtgggc gtgtcgaacc tgttcacgtt
     5461 catgaacacg attccgccct actggaagcc gctgctgccc aagttctacg acatggtcgg
     5521 cgagccccag aaggacaagg agcgactcgc cgcaacgtca cccgccctgc acgccgaccg
     5581 gatcaggacg ccgctgctgg tcgtgcaggg ggcgcgcgac ccgcgcgtga acaaggccga
     5641 gagcgaccag atggtcgagg cgttgcgcaa gcgcggcgtc gacgtcgaat acatcgtcaa
     5701 ggacaacgag ggccacggct tccgcaacga ggagaaccag ttcgagatct acggcgcgat
     5761 ggaagcgttc ttccttcgcc acctgaagcc ggacgcgagc ggctgacgca cgacgcgcgg
     5821 cggccgctgt tttcacgcgg cggcgcggaa cccggagacg ggctccgcgc tttgcgcgtc
     5881 gatcgacggc taggccgcgc gcagttgcgt cacgaccgca gccggcggct cgacaaagag
     5941 ttcgatcgcc tcgttcgccg aggccgcctt cagcagccgc tcctgcacct gcgacggcga
     6001 ccagcgaccg gccggcaccg catcggcgaa gcgcgcggcc gccgcggcgt cgtcgaagaa
     6061 cttgaggaac atctgctcga gctggccgcg atcgcacagg ccgagttcga ggcggaagtc
     6121 gacgcggccc gagcggatcg ctgcctcgtc gatcaggtgc ggatggttgg tggtcaggaa
     6181 cacgacggag ccttcgtgcg cggccacgcc atcgagcgca ttgatgaagc ccgaatagga
     6241 caccttgacg ccggcatcgg ccttgaggcg cgcctggaag aaggcgtcga cgtcctcgat
     6301 caggatcacc gagcgcaccg gcacgctcgc cagcagattg ccgatcttct cgtccgtgac
     6361 gttgggcgag gcgagcgaca gcacgcacac gttcagcgac agttccgagg ccagcgcggt
     6421 gacgagcgag gtcttgccgg ttcccggcgg cccgtacagc aggtagccgc gccgccacgg
     6481 gatgccgagg gcctcgtagc gcgcccgcga ggcgaagaag cgctcgatgt cggcgcggat
     6541 cgcctccttc tggcccgcct tcagcacgac cgaatccagg cgccggttgt tgcccaggcg
     6601 cgcgcgcgtc cactcgttgc tgtacgggct cggcacgtaa acggcgatcc ggttcagttc
     6661 gcgctcgatg cgcgcgttca gcgccgagtt cacgatcgac tccagcgcgc cccggccgcg
     6721 gccgaacgtc gatagcgtga tcttctcgac gactgccacg ccgacctgca cttcgcgctt
     6781 catccacatc agccgcccgt cgagcacgaa gacgtgaaag ccctcgcccg gcgagaacag
     6841 cgccgccggc cggccgccct gctgcagttc gtcgtgcagg ctctggtagc tcgatccctg
     6901 ctggcgcagc gtgcgcacgg tgaagttgtt gatccgccgc agcgcgtcgc ggctgtccat
     6961 gtattcgacc agcgccggaa acatcagctc gtcgcggctg tcgaccgcca gcgtggcgac
     7021 gaagaagtgc tgcccccacg cggcgagctt gccgggaacc tcgcgcagcc acagcgcaag
     7081 gatgcccgcc gccgccaggg cagtgcctcc cgacagcaac ggattcgacg cgatggccga
     7141 ctgcagcgac tcgaacactg gattcccctc tcatggtcgg gtccgggccg gacccgcagc
     7201 gtccgcataa cgcgcggcgt cgcgacggcg gttgaccgaa cgttgccgcg atggcgccgg
     7261 gcggcgcggg cgagccggac gtgccgacat tcgcacgtgc gccggcaaac gcgcatttca
     7321 ggtcgaccgg cacgcggctc tgctccgctt acgtcactgc gccgcgcgca gcctcgctca
     7381 ggcggttccc accgcgacat cctcacagcg cgc
//
LOCUS       DBNHBY010000503         2161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_709533, whole genome shotgun sequence.
ACCESSION   DBNHBY010000503 DBNHBY010000000
VERSION     DBNHBY010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_709533"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC16HB_12335"
     CDS             complement(<1..678)
                     /locus_tag="AC16HB_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR5607620.1"
                     /translation="MTAAAPLCLAIDQGTHASRALVLDAHGQVIARGECAVALKRPRP
                     DWAEQDGEEIVASIHAAVTQAVAGLGTRARAVAVAGLSSQRASAVCWDRASGKPLSPI
                     FSWQDRRMHAWIDQLRAHGDAVHRKTGLFLSPHYGASKLRWAFDHLPAVRQAHDAGTL
                     AWGPMASFLVFRLTKERSFAADPQCAARTQLWNLHARDWDPDLLALFGLPAGGLPCSA
                     PTCQRWGT"
     gene            complement(675..1757)
                     /locus_tag="AC16HB_12340"
     CDS             complement(675..1757)
                     /locus_tag="AC16HB_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HIR5607621.1"
                     /translation="MAGVAIRDVRKSFGAVPVIQGISIDVADGEFVVMVGPSGCGKST
                     LLRMVAGLETVTSGEIAIGDRVVNDLEPKDRNIAMVFQNYALYPHMSVYDNMAYGLKI
                     QGRSRADIAARVQRAADTLELSALLQRRPRELSGGQRQRVAMGRAIVREPAVFLFDEP
                     LSNLDAKLRVQMRSELQALHQRLRTTTLYVTHDQVEAMTLAQRMLVMNAGRSEQIGAP
                     LEVYSKPASTFVAGFIGSPPMNLIPGRIVEGGRTLAGDGDVRMRLPEPRPQLDGRGVL
                     LGVRPEHLEACAPGDALATPDIDFIELLGSDSLVYGHLGANRQGARIAARLHTSVVAK
                     EGQLPLRFAAEHMHLFDPDGGRRIEP"
     gene            complement(1757..>2161)
                     /locus_tag="AC16HB_12345"
     CDS             complement(1757..>2161)
                     /locus_tag="AC16HB_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5607622.1"
                     /translation="LIIPLVASATATFLFRQFFMTIPDELAEAARIDGAGPMRFFRDI
                     VVPLSRTNMAALFVILFIYGWNQYLWPLLITTEESMYTVVIGIRRMIAGGDAATEWNL
                     VMATAMLAMLPPALVVLLMQKWFVKGLVETEK"
BASE COUNT          341 a          771 c          720 g          329 t
ORIGIN      
        1 cgttccccag cgctggcacg tcggcgcgct gcacggcagg ccgccggccg gcaagccgaa
       61 cagtgcgagc aggtccggat cccagtcgcg cgcgtgcagg ttccacagct gggtccgtgc
      121 cgcgcactgc gggtcggcgg cgaacgatct ttccttcgtg aggcggaaca cgagaaagct
      181 cgccatcggt ccccacgcga gcgtccccgc gtcgtgcgcc tgccgcaccg ccggtagatg
      241 atcgaacgcc cagcgcagct tgctcgcgcc gtagtgcggc gacaggaaga gccccgtctt
      301 ccggtgcacc gcgtcaccgt gcgcgcggag ctgatcgatc cacgcatgca tgcgccggtc
      361 ctgccagctg aagatcggcg acagcggctt gccgctggcg cggtcccagc acaccgcact
      421 cgcgcgctgg ctcgacagac cggcaaccgc taccgcgcgc gcgcgcgtgc cgagaccggc
      481 gaccgcctgc gtcaccgcgg catggatcga tgcgacgatc tcctcaccgt cctgctcggc
      541 ccagtcgggc cgggggcgct tgagcgcgac cgcgcactcg ccgcgcgcga tcacctgccc
      601 gtgcgcatcg agcacgaggg cgcggcttgc atgcgtgccc tggtcgatcg ccaggcagag
      661 cggggcagca gcggtcacgg ttcgatccgc cttccgccat cgggatcgaa gaggtgcatg
      721 tgctcggcgg cgaagcgcag cggcagctgg ccttccttgg cgaccaccga agtgtgcagc
      781 cgcgcggcga tgcgcgcgcc ctgcctgttg gcgccaagat gcccgtaaac cagagaatcc
      841 gagccgagca gttcgatgaa gtcgatgtcg ggcgtggcca gcgcatcgcc cggcgcgcag
      901 gcctccagat gctccggccg cacgccgagc agcacgccac ggccgtccag ctgcgggcgc
      961 ggctcgggca gccgcatgcg cacgtcgccg tcgccggcga gagtcctgcc gccctcgacg
     1021 atgcgcccgg ggatcaggtt catcggcggc gagccgatga agcccgcgac gaacgtgctc
     1081 gccggcttgc tgtagacctc gagcggcgcc ccgatctgct cgctgcgccc cgcgttcatc
     1141 accagcatgc gctgcgccag cgtcatcgcc tcgacctggt cgtgcgtgac gtacagcgtc
     1201 gtcgtgcgca gccgctggtg cagcgcctgc agttcgcttc tcatctgcac gcgcagcttg
     1261 gcgtcgaggt tcgacaatgg ctcgtcgaac aggaacaccg ccggttcacg cacgatcgcg
     1321 cgccccatcg ccacgcgctg gcgttgcccg cccgacagtt cgcgcgggcg gcgctgcagg
     1381 agcgcggaca gttcgagcgt atctgccgcg cgctgcacgc gggcagcgat gtcggctcgg
     1441 cttcggccct ggatcttcaa cccgtacgcc atgttgtcgt acacgctcat gtgcggatag
     1501 agcgcgtagt tctggaacac catcgcgatg ttgcggtcct tcggttccag gtcgttgacg
     1561 acgcggtcgc caatcgcgat ctcccccgag gtgaccgtct cgagtcccgc gaccatccgc
     1621 agcagcgtcg acttgccgca gccggacggc ccgaccatca cgacgaactc gccgtcggcg
     1681 acgtcgatcg agatgccctg gatgactggc acggcgccga acgacttgcg cacgtctcgg
     1741 atcgcgacgc cggccatcac ttttccgttt cgacgagacc cttgacgaac cacttctgca
     1801 tcagcagtac cacgagcgcc ggcggcagca tcgcaagcat cgcggtggcc atcaccaggt
     1861 tccactcggt cgcggcgtcg ccgccggcga tcatgcggcg gatgccgatc acgaccgtgt
     1921 acatcgactc ctcggtcgtg atcagcagcg gccacaggta ctggttccag ccgtagatga
     1981 agaggatcac gaacaaggcc gccatgttgg tgcgcgacag cggcaccacg atgtcccgga
     2041 agaagcgcat cggcccggcg ccatcgatgc gcgccgcctc ggccagttcg tccggaatcg
     2101 tcatgaagaa ctggcgaaac aggaatgtcg cggtggcgga cgcgacgagc gggatgatca
     2161 g
//
LOCUS       DBNHBY010000504         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_778027, whole genome shotgun sequence.
ACCESSION   DBNHBY010000504 DBNHBY010000000
VERSION     DBNHBY010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_778027"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="smpB"
                     /locus_tag="AC16HB_12350"
     CDS             complement(<1..269)
                     /gene="smpB"
                     /locus_tag="AC16HB_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR5607623.1"
                     /translation="MSIIQNKKAFHDYSIEERFEAGLVLQGWEVKSIRAGQVHLREAY
                     VIVRDGELYLLNAHVTPLATASTHVQPEPTRTRKLLLRAEEIRKL"
     gene            308..742
                     /locus_tag="AC16HB_12355"
     CDS             308..742
                     /locus_tag="AC16HB_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR5607624.1"
                     /translation="MHRVERSVLVPFSTQQMFELVARVEEYPRFLPWCAASHVRPRVD
                     GSHEATIEIRYHGARGRFTTHNRHYPHERIGMDLVDGPFRRLHGEWTFRELRADACRV
                     HLNLHYQFATGLLGRAIAPVFEEIATTLVDSFTRRAESLNGR"
     gene            732..1034
                     /locus_tag="AC16HB_12360"
     CDS             732..1034
                     /locus_tag="AC16HB_12360"
                     /inference="COORDINATES: protein motif:HMM:NF002490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR5607625.1"
                     /translation="MAAECLVRASVVVAQPDRATVVEVVVPAGATLRDAIVAAGVRSV
                     DELARAGATVGVFGQMRPLDAPIGDGDRIEIFRPLTIDPKESRRLRAALRKSGKTV"
     gene            complement(1031..1576)
                     /locus_tag="AC16HB_12365"
     CDS             complement(1031..1576)
                     /locus_tag="AC16HB_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR5607626.1"
                     /translation="MAKRVHESILAVLMFAFATAAAAQWAWRDDNGRLVFSDKPPPAG
                     VKPNQIVRQPAGAGPVQRSAPLYVTDQPAAKPEPKQAEPKTAGTSAATTPKTLAEREM
                     ESRKRAQERADAEKKAEEEQVRKQQIAQDCERSRGYLRALEDGRRVARTDAQGNQIVL
                     DDAGRAEELARIRSRIASDCN"
     gene            1716..3176
                     /gene="guaB"
                     /locus_tag="AC16HB_12370"
     CDS             1716..3176
                     /gene="guaB"
                     /locus_tag="AC16HB_12370"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR5607627.1"
                     /translation="MRLVQKALTFDDVLLLPAHSTVLPRETQLATKLSRNITLNLPVV
                     SAAMDTVTEAQLAIALAQEGGIGIIHKNLTPQQQAAQVAKVKRFEAGVLRDPITIPPE
                     MRVGDAIALQRTHKFSGLPVVEGKRVVGIVTNRDLRFETRLDAPVREIMTPKERLVTV
                     REGASLDEAKALMHKHRLERVLVVDGAFELRGLITVKDITKATEHPNAAKDEQGKLRV
                     GGAISTGPDSDERAELMVKAGVDVLIVDTAHGHHQGVLDRVRWVKKNYPQVNVIGGNV
                     GTADGARALLEAGADGVKVGIGPGSICTTRIVAGVGVPQITAIANVAKALEGTGLPLI
                     ADGGIRYSGDVAKAIAAGAYAVMMGSMFAGTEEAPGEVILYQGRSYKSYRGMGSLGAM
                     SEGSAARYFQDNEANVDKFVPEGIEGMVPYKGSVLAILFQLAGGLRSSMGYCGCQTIE
                     QMRTRAQFVEITSAGMRESHVHDVKITKEAPNYRIE"
BASE COUNT          534 a         1077 c         1119 g          547 t
ORIGIN      
        1 atcagcttgc ggatctcctc ggctcgcagc aacagcttgc gcgtgcgcgt cggttctggc
       61 tgcacgtgcg tggacgcggt cgcgagcggc gttacatgcg cattcagcag gtacaactcg
      121 ccgtcgcgca cgatgacgta cgcctcgcgc aaatggacct gaccggcgcg gatcgacttg
      181 acttcccagc cttgaagcac gagcccggcc tcgaagcgct cttcgatcga gtagtcgtga
      241 aaggccttct tgttctggat aattgacatc ggttgccacg caagttcgcc cgaataatcg
      301 cacgctcatg catcgcgtcg aacgcagtgt cctggtgccg ttcagcacgc aacagatgtt
      361 cgagctggtg gcacgcgtcg aggagtaccc caggttcctg ccgtggtgtg ctgccagcca
      421 cgtgcgcccg cgcgtcgacg gcagtcacga ggcgacgatc gaaatccgct accacggcgc
      481 ccgcggccgc tttaccacgc acaaccggca ctacccgcac gagcgcatcg gcatggacct
      541 cgtcgacggg ccgtttcgcc gcctgcatgg cgaatggacc ttccgcgaac tgcgcgccga
      601 tgcctgccgc gtgcacctga acctgcacta ccagttcgcc accggcctgc tcggccgcgc
      661 gatcgcgccg gtgttcgaag agatcgcgac gaccctggtg gattccttca cgcgccgtgc
      721 ggagtcgctg aatggccgct gagtgcctcg tgcgcgcgag cgtcgtggtt gcgcaaccgg
      781 accgggcaac ggtcgtcgag gttgtggttc ccgccggcgc gacgctgcgc gacgcgatcg
      841 ttgccgcggg tgtgcgttcg gtcgacgaac tggcacgcgc aggggccacg gtcggcgtgt
      901 tcggccagat gcggccgctc gatgccccga tcggcgacgg cgaccgtatc gagatctttc
      961 ggccgttgac gatcgatccc aaggagtcgc gccgcctgcg cgcggcgctg cgaaaatcgg
     1021 gcaagacggt ttagttgcag tcgctcgcga tgcgcgagcg gatgcgggcc agttcttcgg
     1081 cgcggccggc atcgtcgagc acgatctgat tgccctgcgc atcggtgcgc gcgacgcgac
     1141 ggccgtcctc gagcgcgcgc aggtagccgc gcgagcgctc gcaatcctgc gcgatctgct
     1201 gcttgcgcac ctgttcctcc tcggctttct tttctgcgtc ggcccgttcc tgcgcgcgct
     1261 tgcgcgattc catttcgcgc tcggccagcg ttttcggtgt cgtcgcagcg gaggtcccgg
     1321 cggtcttcgg ctcggcctgc ttcggctccg gctttgcggc gggctgatcg gtaacgtaca
     1381 ggggtgcaga ccgctgaacc gggccggcac ctgccggctg ccgcacgatc tggttcggct
     1441 tcactccagc cggcggcggc ttgtcggaga acaccagccg tccgttgtcg tcgcgccagg
     1501 cccactgtgc ggcggctgcc gtggcgaacg cgaacatcag gacggcgagg atcgactcgt
     1561 ggacgcgctt tgccatggcg gctccttggt tggcgcggat tgtgcggccc ggcagagggt
     1621 gccgcaatag tccgtcggac cgatccataa cggggcgccg gccgcgcgcg ccgacgcgtg
     1681 tgcgtataat tttcttttgc gtcacgaggc tccccatgcg gctagtccag aaggcactga
     1741 ccttcgatga cgttcttctc ctgccggctc attcgaccgt cctcccgcgc gagacccagc
     1801 tcgcgaccaa gctctcccgc aacatcacgc tgaacctgcc ggtcgtgtcg gcggcgatgg
     1861 acacggtcac agaggcccag ctcgcgatcg cgctggcgca ggagggcggc atcggcatca
     1921 tccacaagaa cctgaccccg cagcagcagg cggcccaagt cgcgaaggtg aagcgtttcg
     1981 aggccggcgt gctgcgcgac ccgatcacga tcccgccgga gatgcgcgtg ggcgacgcga
     2041 tcgccctgca gcgcacgcac aagttttccg gcctgccggt ggtggaaggc aagcgcgtgg
     2101 tcggcatcgt caccaaccgc gacctgcgct tcgaaacgcg gctggatgcg ccggtgcgcg
     2161 agatcatgac gccgaaggag cggctggtca ccgtgcgcga gggcgcaagc ctcgacgagg
     2221 ccaaggcgct gatgcacaag caccggctcg agcgcgtact ggtcgtcgac ggcgcgttcg
     2281 agctgcgcgg gctgatcacg gtcaaggaca tcaccaaggc gacggagcac ccgaatgcgg
     2341 ccaaggacga gcagggcaag ctgcgcgtcg gtggcgcgat cagcaccgga ccggactccg
     2401 acgagcgggc cgaactgatg gtcaaggccg gcgtggacgt gctgatcgtc gacaccgcgc
     2461 atggccacca tcagggcgtg ctcgatcgcg tgaggtgggt caagaagaac tatccgcagg
     2521 tcaacgtgat cggcggcaat gtcggcacgg ccgacggcgc gcgcgctctg ctcgaagccg
     2581 gcgccgatgg cgtgaaggtc ggcatcggcc ccggctcgat ctgcacgacg cggatcgtgg
     2641 ccggagtggg tgtgccgcag atcaccgcga tcgcgaacgt ggccaaggcg ctcgaaggca
     2701 ccggattgcc gctgatcgcc gatggcggca tccgctactc cggcgatgtc gcgaaagcca
     2761 tcgcggccgg cgcctatgcg gtgatgatgg gcagcatgtt cgccggcacc gaggaggcgc
     2821 cgggtgaggt gatcctgtac cagggccgct cgtacaagag ctaccgcggc atggggtcgc
     2881 tcggcgcgat gagcgagggc tctgccgcgc gctactttca ggacaacgaa gccaacgtcg
     2941 acaagttcgt gcccgaaggc atcgaaggca tggtgccgta caagggcagc gtgctggcga
     3001 tcctgttcca gcttgccggc gggctgcgct cgagcatggg ctactgcggc tgccagacga
     3061 tcgagcagat gcgcacccgc gcccagttcg tcgagatcac cagcgccggc atgcgcgagt
     3121 cgcacgtgca cgacgtgaag atcaccaagg aagcgcccaa ctaccggatc gagtagcgaa
     3181 cttgagccac gaacgaatcc tgatcctcga cttcggcgcg cagtacacgc aattgatcgc
     3241 gcggcgcgtg cgtgaggcga aggtctactg cgagatc
//
LOCUS       DBNHBY010000505         2199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_371816, whole genome shotgun sequence.
ACCESSION   DBNHBY010000505 DBNHBY010000000
VERSION     DBNHBY010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_371816"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="AC16HB_12375"
     CDS             complement(<1..1646)
                     /locus_tag="AC16HB_12375"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5607628.1"
                     /translation="MPKLFALVVAALVVGVGDATYAQTDASAGARPKVGLVLSGGGAR
                     GFAHIGVLKVLQELHVPVDVIAATSMGSIVGGAYAAGYSPRQLKELVRKTDWNTMFAR
                     RAPRADLAWRLKEDDYRNLFTFEFGIKEFGLTLPRGAVGAQELGLMLRALGGVVKDVT
                     DLSELPIPFAAAATDLATGKLVVLQKGASLATAMRASMSVPGAFAPIDFQGRPLVDGA
                     LVRNLPVDIARRMGADIVIAVNVVTPLLPRDKLTDVIAVGQQMINILTEQNVERSLAE
                     LTPRDILIAPNLAEFTASDFGKGEAIVAAGEAAARAAAQRLAQLSLNPERYAQAERAR
                     TAWLHADVPFPVVDVRVEGLRTVNPASVLAEIELPRDVPVDTAEVQRQVRRVYASGDF
                     ESVSYNLLDAPGGRVLIVTPFEKSWGYNSLQLGGSLETNFGNDNRFNLLALHTWSWLN
                     RAGASWRNELQFGTVRRFGSEFLQPFAAGSRWLVVPRVRIERESFDRFDGGRAVAQFE
                     SRENFADLSLRYLLGSLGMAQVGIGHARTETRLLSGAPGLT"
     gene            1697..>2199
                     /locus_tag="AC16HB_12380"
     CDS             1697..>2199
                     /locus_tag="AC16HB_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5607629.1"
                     /translation="MAARTGARILVDQLLVQGVNHAFCVPGESYLAVLDALHDTSAIK
                     LIINRHENGSAFMADAYAKLTGQPGVAFVTRGPGATNASIGVHTAFQDSTPIVVFIGQ
                     VGSDFVEREAFQEIDYRRMFGQMTKWVAQIDRADRIPEYVARAFQVATSGRPGPVVLA
                     LPEDMQLA"
BASE COUNT          366 a          780 c          711 g          342 t
ORIGIN      
        1 gtcagccccg gagcgccgct gagcagccgc gtctcggtac gcgcatggcc gataccgacc
       61 tgcgccatgc caaggctgcc cagcagatag cgcagcgaaa ggtccgcgaa gttctcgcgc
      121 gattcgaact gcgcgacggc gcggccgccg tcgaaccgat cgaacgattc gcgctcgatc
      181 cgcacccgcg ggacgacgag ccagcggctg ccggccgcga atggctgcag gaactcggag
      241 ccgaaccggc gcacggtgcc gaattgcagt tcgttgcgcc acgaggcgcc ggcgcggttc
      301 agccacgacc acgtgtgcag tgccagcagg ttgaagcgat tgtcgttgcc gaagttggtt
      361 tccaggctgc cgcccagttg cagcgagttg tagccccagc tcttctcgaa cggcgtaacg
      421 atcagcacgc gcccgccggg cgcgtcgagc aggttgtagc tgaccgactc gaagtccccg
      481 ctcgcgtata cgcgccggac ctgccgctgc acctcggcag tgtcgaccgg cacatcgcgc
      541 ggcagttcga tctccgccag cacgctggcc gggttgaccg tgcgcagtcc ctccacgcgc
      601 acgtcgacga ccggaaacgg cacgtccgca tgcagccacg cagtgcgtgc gcgctcggcc
      661 tgcgcatagc gctcgggatt gagcgacagt tgcgcgagcc gctgcgccgc cgcccgcgcc
      721 gccgcctcgc cggccgccac gatcgcctcg cccttgccaa agtcgctggc agtgaactcc
      781 gccagattgg gcgcgatcag gatgtcgcgc ggcgtcagct cggcgaggct gcgctcgacg
      841 ttctgctcgg tcaggatgtt gatcatctgc tggccgaccg cgatcacgtc ggtcagcttg
      901 tcgcgcggca gcagcggcgt gacgacgttc accgcgatca cgatgtccgc gcccatccgg
      961 cgcgcaatgt cgaccggcaa gttgcgcacc agcgcgccgt cgaccagcgg ccggccctgg
     1021 aaatcgatcg gcgcgaacgc cccgggcacc gacatcgacg cgcgcattgc ggtggcaagc
     1081 gacgcaccct tctgcagcac gacgagcttg cccgtcgcga gatccgtggc cgccgccgcg
     1141 aacggtatcg gcagctccga caggtcggtc acgtccttca ccacgccgcc gagcgcccgc
     1201 aacatcagcc cgagctcctg cgcgccgacc gcgccgcgcg gcaacgtcag tccgaattcc
     1261 ttgatgccga actcgaacgt aaagaggttg cggtagtcgt cttccttcag ccgccacgcg
     1321 aggtcggcgc gcggtgcgcg gcgcgcgaac atcgtgttcc agtcggtctt gcgcaccagt
     1381 tccttgagct ggcgcggcga atagccggcc gcgtatgcgc cgcccacgat cgaacccatg
     1441 ctggtggcgg cgatcacgtc gaccggcacg tgcagttcct gcagcacctt cagcacgccg
     1501 atgtgcgcga acccgcgcgc gccgccgccc gagagcacca ggcccacctt gggacgcgcg
     1561 ccagcggatg catcggtctg tgcatacgtc gcatcgccga cgccgacgac gagcgccgcg
     1621 acgacaaggg caaagagctt cggcattcgg aagcacactg ttattcttcc gccgccgcaa
     1681 tctacaagga tttgcgatgg ccgcgcgcac cggtgcccgc attcttgtcg accagctgct
     1741 ggtccaggga gtcaaccacg ctttctgcgt gccgggcgag agctatctgg cagtgctgga
     1801 tgcgctgcat gacacgagcg cgatcaagct catcatcaat cgtcacgaga atggctcggc
     1861 cttcatggcc gacgcatacg caaaactcac tggccagccg ggcgtcgcat tcgtcacgcg
     1921 cggccccggg gcaaccaatg cctcgatcgg cgtgcacacc gcgtttcagg actccacgcc
     1981 gatcgtcgtg ttcatcggcc aggtcgggag cgatttcgtc gagcgggagg cgttccagga
     2041 gatcgattac cggcgcatgt tcggccagat gaccaagtgg gtcgcgcaga tcgatcgtgc
     2101 cgatcgcatc cccgaatacg tggcgcgcgc attccaggtc gccaccagcg gccggccggg
     2161 accggtcgtg ctcgcactgc cggaggacat gcagctcgc
//
LOCUS       DBNHBY010000506         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_236901, whole genome shotgun sequence.
ACCESSION   DBNHBY010000506 DBNHBY010000000
VERSION     DBNHBY010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_236901"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16HB_12385"
     CDS             complement(<1..695)
                     /locus_tag="AC16HB_12385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5607630.1"
                     /translation="MNQRQLSDSWERGDPYERYVGRWSRRVAPRFLSWLGTPAGCDWL
                     DVGCGTGALCAAILDQCAPKSVVGVEPSAGFLETAQERLAGRAQLKQGSATSIPLDDV
                     SVDAVVSGLVLNFVAEPRAALAEMARVARRGGTIAAYVWDYAGQMELMRIFWDAAVAL
                     DPSAAKLDEGVRFPLCRRDALAALFADARLSGTEATAIDIETTFADFDDYWQPFLGGT
                     GPAPAYAMSLDEPA"
     gene            complement(932..2251)
                     /locus_tag="AC16HB_12390"
     CDS             complement(932..2251)
                     /locus_tag="AC16HB_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR5607631.1"
                     /translation="MLEIAVVALAIALVVALIALVLLRRGALAGVESELRRLQDEQGE
                     VNERLERELRGAIDAAATATRMETGARFTELQSAINVQTTQANTALAQNLNAYGQTMQ
                     QQLAELTRAIQLNLADVRGTLQTQLASLQSDNSAKLEQMRATVEEKLQTTLEARLGES
                     FRLVSERLEQVHRGLGEMQGLAQGVGDLKKVLGNVKTRGTFGEVQLGALLDQVLAPDQ
                     YASNVATRPGSTERVEFAVRMPGRSGDGETVCWLPIDAKFPSEDYERLLAAQEAADPA
                     AVEVAARAIEARIRLEARTIRDKYVEPPHTTDFAILFLPTEGLFAEVLRRPGLTEALH
                     REHRIVVAGPTTLFAMLNSLQMGFRTLALEKRSAEVWQVLGAVKTEFGKFGDVLSRLK
                     QQLATASNTIETAETRTRQMSRALRGVEALPVEQAARLLPPERSEEG"
BASE COUNT          354 a          846 c          742 g          365 t
ORIGIN      
        1 gcgggctcgt cgagcgacat cgcgtaggcg ggcgccggac ccgtgccgcc gaggaacggc
       61 tgccagtagt cgtcgaagtc cgcaaaggtc gtctcgatgt cgattgcggt cgcctcggtt
      121 ccactgagtc tcgcgtccgc gaacaaagcg gccagcgcat cgcgccggca cagcggaaag
      181 cgcacgcctt cgtccagctt cgcggcgctc gggtcgagcg cgaccgccgc atcccagaag
      241 atgcgcatca gctccatctg gcccgcgtag tcccacacat aggccgcgat cgtgccgccg
      301 cggcgcgcaa cgcgcgccat ctccgcgagc gccgcgcgcg gctcggcgac gaagttcagc
      361 accaggcccg agaccaccgc atcgaccgat acatcgtcca gtggaatcga agtcgcactg
      421 ccctgcttca gttgcgcgcg acccgcgagt cgctcctgcg ccgtctcgag aaagcccgcc
      481 gaaggctcga cgccgacgac ggacttgggt gcacactggt cgaggatcgc cgcacacagc
      541 gcgccggtgc cacagccgac gtccaaccag tcgcaaccgg cgggagtgcc gagccacgac
      601 aggaaccgcg gcgcgacgcg gcggctccag cgacccacgt agcgttcgta cggatcgccg
      661 cgttcccacg aatccgagag ttgtcgctga ttcatccgtc actccttccc gcgcagcgtc
      721 cgcggtcgca agtgaaagtc gcacagccaa aggacgcgag cacggtcgcc gtccagtctg
      781 actcagctcg atgcctgccg gatgaccact ttcacgcgac gacggtcacc aatgccaaag
      841 tgcatgcggg acggcggaca gcgtagcggg tgagcctatg aaaccccatg ctgaggcgca
      901 agcgccgagc cgcagccccc gcgtcacccc ctcacccctc ctcgcttctc tccggcggca
      961 gcagccgcgc ggcctgctcc acgggcagcg cctccacgcc ccgcagcgcc ctgctcatct
     1021 gccgggtgcg cgtctcggcg gtttcgatcg tgttcgatgc ggtcgcaagc tgctgcttca
     1081 gcctcgacag cacgtcgccg aatttgccga actcggtctt caccgcgccg agcacctgcc
     1141 acacctcggc actgcgcttc tccagcgcga gcgtgcggaa gcccatctgc aggctgttca
     1201 gcatcgcgaa gagcgtggtc ggcccggcga cgacgatgcg gtgctcacgg tgcagcgctt
     1261 cggtcagccc cggccggcgc agcacctcgg caaacaggcc ctcggtcggc aggaacagga
     1321 tggcgaagtc ggtcgtgtgc ggcggctcga cgtacttgtc gcggatcgtc ctggcctcga
     1381 gccggatgcg cgcctcgatc gccctggccg ccacttccac agcggccgga tcggcggcct
     1441 cctgcgcggc gagcagccgc tcgtaatcct cgctcggaaa ctttgcgtcg atcggcagcc
     1501 aacagacggt ctcgccgtcg ccgctgcggc ccggcatccg caccgcgaac tcgacgcgct
     1561 cggtcgatcc cggccgtgtc gcgacattgg acgcgtactg gtccggtgcg agtacctggt
     1621 ccagcaacgc gccgagctgc acctcgccga aagtgccgcg cgtcttcacg ttgccgagca
     1681 ccttcttcaa gtcacccacg ccctgcgcca gtccctgcat ctcgccaagc ccgcggtgca
     1741 cctgctcgag ccgctcggac acgaggcgga acgactcgcc cagtcgcgcc tcgagcgtgg
     1801 tctgcagctt ctcctcgacg gtcgcgcgca tctgctcgag cttcgccgag ttgtcgctct
     1861 gcaggctggc gagctgcgtc tgcagcgtgc cgcgcacgtc ggcgaggttg agctggatcg
     1921 cgcgcgtgag ttcggcgagc tgctgctgca tcgtctggcc gtaggcgttg aggttctgtg
     1981 ccagcgcggt gttggcctgc gtcgtctgga cgttgatcgc cgattgcagc tcggtgaagc
     2041 gcgcgccggt ttccatgcgc gtggcggtcg ccgcggcgtc gatcgcgccg cgcagttcgc
     2101 gctcgagccg ctcgttgacc tcgccctgct cgtcctgcag gcgccgcagc tcggactcga
     2161 ctccggccag cgcgccgcgg cgcaggagca cgagcgcaat cagcgcgacg accagcgcga
     2221 tcgcgagcgc gacgacggcg atttccagca tccccactcc ccaaatgaag caggggcgat
     2281 cttagagctt gtccgtgagc aattcgt
//
LOCUS       DBNHBY010000507         2098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1117420, whole genome shotgun sequence.
ACCESSION   DBNHBY010000507 DBNHBY010000000
VERSION     DBNHBY010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1117420"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16HB_12395"
     CDS             complement(<1..417)
                     /locus_tag="AC16HB_12395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR5607632.1"
                     /translation="MTAVPYSLDRVDAIVDRYRAARTNLVQILREVQEPDGWIRPEAI
                     TRIAQRVQLPRARVEGVAGFYSFLHLMPGGRYRVRFSDNIIDRMAGSLALLERLCAQL
                     WVERGKVSEDHLVSVDTTSCTGMGDQAPALLVNGRAI"
     gene            complement(499..777)
                     /locus_tag="AC16HB_12400"
     CDS             complement(499..777)
                     /locus_tag="AC16HB_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR5607633.1"
                     /translation="MAEQKNADESRRDLVLRLKRVEGQIRGVQGMVEDGADCEEIAQQ
                     LTAARKALDRAFYELMACAIKHPQFSANQREKQDARIERLARVLAKLG"
     gene            complement(785..1006)
                     /locus_tag="AC16HB_12405"
     CDS             complement(785..1006)
                     /locus_tag="AC16HB_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR5607634.1"
                     /translation="MTVDRLIRIFAGSFILLSLALGIEGSPIFLSKWWLAFTAFVGLN
                     LFQYGFTNACPLGWILKKLGVPESRVACA"
     gene            complement(1010..>2098)
                     /locus_tag="AC16HB_12410"
     CDS             complement(1010..>2098)
                     /locus_tag="AC16HB_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5607635.1"
                     /translation="PDIVAIDDTTLDAAPRIVLRVDAAKAALYGIAAADVARTAQLAL
                     AGEDITPLHDGFSKYAVPVRVAIAPEKMGRLDDVLKLRLRGQDEKGQPYEVALAELVR
                     PVTLPAERVIYRKDLQPVVYVVGDVAGGKGKTDSPLYGMFELRDGIAKLATPGGLPLA
                     EHWIAQPSDRFREFGLKWDGEWQITYETFRDMGIAYGVGLILIYLLVVAQFRSYLTPL
                     VIMAPIPLTIIGVMPGHALLGSQFTATSMIGMIALAGIIVRNSILLVDFIHQRLDEGA
                     DFQQATIDAAATRAKPIVLTAVAAMLGAIFILDDPIFNGLAISLLFGIFVSTALTLIV
                     IPVLYYAANWRKEARRVHVPIHPQPQGA"
BASE COUNT          347 a          703 c          710 g          338 t
ORIGIN      
        1 gatggcgcgg ccattgacca gcagcgcggg cgcctggtcg cccatgccgg tgcacgaagt
       61 ggtgtcgacc gacacgaggt gatcctccga caccttgccg cgctcgaccc acaactgcgc
      121 gcacaggcgc tcgaggagcg ccaggctgcc cgccatgcgg tcgatgatgt tgtcggagaa
      181 gcggacgcga tagcgaccgc ccggcatcag gtgcagaaaa ctgtagaaac ccgcgacgcc
      241 ctcgacgcgc gcgcgcggca actgcacgcg ctgcgcgatg cgcgtgatcg cctcgggacg
      301 gatccagccg tccggttcct gcacttcgcg caggatctgc accagattgg tacgggccgc
      361 gcggtagcgg tcgacgattg catcgacccg atcaagggaa tacgggactg ctgtcatgca
      421 gcccaatgct cgcgctgccg ccccggtgac aagtgaccga gatcaatgat gcgagtggca
      481 cggcgccggg ttgttgctct atccgagctt tgccagcacg cgcgccagcc gttcgatgcg
      541 agcgtcctgc ttctcgcgct ggttggcgga gaactgcggg tgcttgatcg cgcacgccat
      601 cagctcgtag aaggcgcggt cgagcgcctt gcgcgcggcg gtcagttgct gcgcgatctc
      661 ctcgcagtcg gcgccgtcct ctaccatgcc ctgcacgccg cggatctgcc cttcgacgcg
      721 cttgagccgc agcacgaggt cgcggcgcga ctcgtcggcg ttcttctgct cggccatggt
      781 cgcgttacgc gcaggcgacg cgcgactcgg gcacgccgag cttcttcagg atccagccga
      841 gcgggcacgc gttggtgaag ccgtactgga acaggttcag cccgacgaac gccgtgaacg
      901 ccagccacca tttggagagg aagatcgggc tcccctcgat gcccagcgcg agcgacagca
      961 ggatgaaact gccggcgaag atgcggatca gacggtcgac ggtcatggct caggctccct
     1021 ggggttgcgg atggatcgga acatggacgc ggcgcgcttc cttgcgccag ttcgccgcgt
     1081 aatagagcac cgggatcacg atcagggtca gcgccgtcga cacgaagatg ccgaacagca
     1141 gcgagatcgc gagcccgttg aagatcgggt cgtcgaggat gaagatcgcg ccgagcatcg
     1201 ccgcgaccgc ggtcagcacg atcggcttgg cgcgcgtggc ggcggcatcg atggtcgcct
     1261 gctggaagtc ggcgccttcg tcgagccgct ggtggatgaa gtcgaccagc aggatcgagt
     1321 tgcgcacgat gatgccggcg agcgcgatca tgccgatcat cgacgtcgcg gtgaactggc
     1381 tgcccagcag cgcgtgtccc ggcatcacgc cgatgatcgt cagcgggatc ggcgccatga
     1441 tgacgagcgg cgtcaggtac gagcggaact gcgcgaccac cagcaggtag atcaggatca
     1501 gccccacgcc gtacgcgatg cccatgtcgc ggaacgtctc gtaggtgatc tgccactcgc
     1561 catcccactt caggccgaac tcgcggaagc ggtccgacgg ctgcgcgatc cagtgctcgg
     1621 cgagtggcag cccgcccggc gtggcgagtt tcgcgatgcc gtcgcgcagc tcgaacatcc
     1681 cgtatagcgg cgagtcggtc ttgcccttgc cgcccgcgac atcgccgacc acgtacacga
     1741 ccggctgcag gtccttgcga tagatcacgc gctccgcggg cagcgtgacc ggacgcacca
     1801 gttccgccag cgcgacctcg tacggctggc ctttctcgtc ctgcccgcgc agcctgagct
     1861 tcagcacgtc gtccagccgc cccatttttt ccggcgcgat cgccacccgc accggcactg
     1921 cgtacttgct gaacccgtcg tgcagcggcg tgatgtcctc accggcgagc gccagttgcg
     1981 ccgtacgggc gacatcggcc gcggcgatgc cgtacagcgc ggccttggcc gcgtccacgc
     2041 gcagcacgat gcgcggtgcg gcgtcgagcg tggtgtcgtc gatcgcgacg atgtcggg
//
LOCUS       DBNHBY010000508         1940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1319649, whole genome shotgun sequence.
ACCESSION   DBNHBY010000508 DBNHBY010000000
VERSION     DBNHBY010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1319649"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="AC16HB_12415"
     CDS             <1..699
                     /locus_tag="AC16HB_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5607636.1"
                     /translation="AGLVTLNQRVLLVDLDPQGNATMGSGIDKRELRQSVYQVLLGLA
                     TIRQAVSRSETGMYDVLPSNRELAGAEVELVEVDRRELRLKDALAAVEADYDYILIDC
                     PPSLSLLTLNGLCATHGVIIPMQCEYFALEGLTDLVNTIKRVHANLNPQLAIIGLLRV
                     MFDPRMTLQQQVSDQLAAHFGDKVFNTIIPRNVRLAEAPSYGMPGVVYDAASKGAQAY
                     TAFGREVIERMKTA"
     gene            701..1531
                     /locus_tag="AC16HB_12420"
     CDS             701..1531
                     /locus_tag="AC16HB_12420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF049302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607637.1"
                     /translation="MLDPGTIAPELRMPSDVALARVEEAGLNAAQPSEQLLVDGWLLR
                     FSPGKARRARSVNAIGAGRLDLEAKLQLCRKFYERARLPLLFRITPFSQPAVLDAFLQ
                     RNGFEAFDQTRVMTRALGAEHPVEGSARASGRFSSVAVEVDEFARIVGALRGSPEQQI
                     RAHESRLRSSPLQHASVRRILRIDGEAVAAGQTVIEDDVAGLYDIVTAPPWRGRGLGR
                     LLSALLLADARAGGAVTAYLQVDAGNEPARRIYAALGFVDRYAYWYRRPAGVVDELLH
                     "
     gene            1542..>1940
                     /locus_tag="AC16HB_12425"
     CDS             1542..>1940
                     /locus_tag="AC16HB_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5607638.1"
                     /translation="MIKRPKGLGRGLDALLGGSSDRVAASAGVSTLPLSHLQPGRYQP
                     RTRMNSEALTELADSIRAQGVMQPILVRSVGHDRYEIIAGERRFRAAQMAGLAEVPVL
                     IRNVPDESALAMALIENIQREDLNALEQAQG"
BASE COUNT          305 a          628 c          688 g          319 t
ORIGIN      
        1 gcgggcctcg tcacgctgaa ccagcgcgtg cttctggtcg acctcgatcc gcagggcaac
       61 gccaccatgg gcagcggcat cgacaagcgc gagctcaggc aatccgtcta tcaggtgctg
      121 cttgggctgg caacgatcag gcaggcggtg tcgcggtccg agaccggcat gtacgacgtg
      181 ctgccgagca atcgcgagct ggcgggtgcg gaggtcgaac tggtcgaggt cgatcggcgc
      241 gagcttcgcc tgaaggacgc gctggccgcc gtcgaagccg actacgacta catcctgatc
      301 gattgcccgc cgtcgctgtc gctgctcacg ttgaacggcc tgtgcgccac gcacggcgtg
      361 atcatcccga tgcagtgcga gtacttcgcg ctcgaggggc tgaccgatct ggtgaacacc
      421 atcaagcgtg tgcacgcaaa cctgaatccg cagctggcga tcatcggcct gttgcgggtg
      481 atgttcgacc cgcgcatgac gttgcagcag caggtgtcgg accaactggc cgcgcatttc
      541 ggcgacaagg tcttcaatac gatcatcccg cgcaacgttc ggctggcgga ggcgccgagc
      601 tacggcatgc cgggcgtcgt gtatgacgcc gcttccaagg gtgcgcaggc ctacaccgcg
      661 ttcgggcgcg aagtgatcga acgcatgaag acggcctgag atgctcgacc ccggcaccat
      721 cgcccccgaa ttgcggatgc cctcggatgt cgcgctcgcg cgcgtcgagg aagcggggct
      781 gaatgcggcg cagccgtcgg agcagttgct cgtcgatggc tggctgttgc gcttctcgcc
      841 gggcaaggcg cgccgcgcac ggtcggtcaa tgcgatcggc gcggggcggc tcgacctcga
      901 agcgaagttg cagctgtgcc ggaaattcta cgagcgggcg cgactgccgc tgctgttcag
      961 gatcacgccc ttttcgcagc cggctgtgct cgatgcgttt ctacagcgga acggcttcga
     1021 ggcgttcgat caaacgcgcg tgatgacacg cgcactgggc gcggagcatc ccgtcgaggg
     1081 cagcgcccgg gcctcgggtc ggttcagttc cgttgcagtc gaagtcgacg agttcgcgcg
     1141 catcgtcggc gcgctgcgcg gttcgcccga gcagcagatc cgcgcgcatg aatcgcgcct
     1201 gcgctcttcg cccctgcagc acgcgtcggt ccggcgcatc ctgcgcatcg acggcgaggc
     1261 ggtcgccgcc gggcagacgg tgatcgagga cgacgtcgcc ggcctgtacg acatcgtgac
     1321 cgcgccgccg tggcgcggcc gcgggctcgg acgcctgctg agcgcgctgc tgcttgcgga
     1381 cgcgcgcgcg ggcggcgcgg tcaccgcgta cctgcaggtg gacgcgggta acgaacccgc
     1441 gcggcgcatc tacgcggcgc tgggtttcgt cgaccgctat gcctactggt atcgacgacc
     1501 ggccggcgtc gtcgacgaat tgctgcatta gggagactgg aatgatcaag cggcccaagg
     1561 gcctcggacg cggactcgac gcactgctgg gcggcagttc cgatcgtgtc gcagcgtcgg
     1621 ccggcgtcag cacgctgccg ctgtcgcatt tgcagccggg ccgataccag ccgcgcacgc
     1681 ggatgaactc cgaagcgctg accgaactcg ccgattcgat ccgcgcgcag ggcgtgatgc
     1741 agccgatcct ggtgcggtcg gtcggccatg accgctacga gatcatcgcg ggcgaacgcc
     1801 ggttccgcgc cgcgcagatg gcgggtctcg ccgaggtgcc ggtgctgatc cgcaatgtgc
     1861 ccgacgaatc ggcgctggcg atggcgttga tcgagaacat ccagcgcgag gacctgaatg
     1921 cgctggagca ggcgcagggg
//
LOCUS       DBNHBY010000509        14169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1589786, whole genome shotgun sequence.
ACCESSION   DBNHBY010000509 DBNHBY010000000
VERSION     DBNHBY010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1589786"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="AC16HB_12430"
     CDS             <1..600
                     /locus_tag="AC16HB_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607639.1"
                     /translation="PSGKTYASAGVQCALNPISGMGPMIQAGLYNPKKSASAAVSLNG
                     TPAATVTFLSPDAAVWLTNGMNTVVVALDKKTADSYSFDASSTQQNVCIPDTSGNTVS
                     GDLELAGSGKSYATVTPGCALNSLTGRAQPYVNFFDNGAYLLNVSVNNVPLTQLNGTT
                     RRSTPVFLSAGLNVLSAANGSISTDYYVRDGGTGSCTLP"
     gene            complement(878..1537)
                     /locus_tag="AC16HB_12435"
     CDS             complement(878..1537)
                     /locus_tag="AC16HB_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR5607640.1"
                     /translation="MPSASLWTLSNAQFAIEAVAIVAFALSGLLAAARKHLDAVGVCV
                     VTGLAAFGGGTLRDILLDRRPFFWVAHAAWLWAIIALAIGAMIFLRARYFEPTERAMQ
                     WPDALGLGLFTASGTQIALEAMLPSIVAVLMGMVTAVFGGVLRDIVCNEIPSAFRDHR
                     PYAVCSFIGGWVLVFAQQFGASEWTALVAASAAATALRGLALYTGYTLPSWQRGEPRR
                     H"
     gene            2028..3452
                     /locus_tag="AC16HB_12440"
     CDS             2028..3452
                     /locus_tag="AC16HB_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5607641.1"
                     /translation="MAVGCLLLALNGCASVAPQSAPFAAVDIPAAWSAADPSATTDTS
                     SLSQWWLRFDDPLLGNLVNQALQANTTVKSAQAALRQARALRDVSAAGLLPAVGSSAS
                     AQRSTSGSNDATNNFRVGLDASWELDIFGANRSALDASNAAVQASAASLGDVQVSIAA
                     EVALGYITLRGAQARLAIADENLASQLETLQITQWRLQAGLVTSLEAEQARAAAEQTR
                     AQLPALQTSIAQTRHALAVLTGQPPAALATLLATMSPVPQAADGLVLSIPAETLRQRP
                     DVRAAEQQVAAAIARVAQADAARMPNFKLGGSLGLNALTLGSLTNGASVVSSLLASVS
                     MPVFDGGALRAQVRAQQAALAQAGAAYQATVLTALKDVEDALIALRGDRERLLRLQHA
                     AEAASNAALLARQRFSSGLVDFQTVLETQRTQLNTQDSVASAGADLSADHVRLYKALG
                     GGWRPEDSDATPSSTEDAPRIPRS"
     gene            3449..4783
                     /locus_tag="AC16HB_12445"
     CDS             3449..4783
                     /locus_tag="AC16HB_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5607642.1"
                     /translation="MNTPNPSATVAPAPEVSTETDLATLLEEPSSRAWYRRPAVWAGV
                     LLLAVVAAGLWYWQARQTASAAPSYTTHTVARGNLTLSVTANGTLQPTRSISIGSELS
                     GTVLKVNVDVNDRIRKGQVLVELDTSKLRDQILRSRASLAAANAKVAQTVATVKEAKA
                     SLARFEEVARLSGGKVPSKAELDSARAALDRAIADEASARASVADAQAALSTDEINLS
                     KASISAPSDGVVLTRSVDPGNAVAASLQAVTLFTVAEDLQKLRLWVYVDEADVGSVAA
                     GQEATFTVSAYPSRKFPARVTRVGFGSTITDNVVTYLTYLDVDNSDLSLRPGMTATAT
                     IIATQRTDVLLVPNTALRFAPTAAGAGAPAAKGGIVSTLIPRMPRSSTRTSAAAGAST
                     AAAREVWVLRDGAAVPVAVIPGISDGRMTEITGGDLQAGMQVITDQKTVTAK"
     gene            4849..5631
                     /locus_tag="AC16HB_12450"
     CDS             4849..5631
                     /locus_tag="AC16HB_12450"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607643.1"
                     /translation="MPPLINAPAAAPPVKAGTPLIRLRGVTKRYGTGQAELMALKGID
                     LDITAGEFVAIMGPSGSGKSTAMNILGCLDTPSAGQYLFHGAHVEALSRDQRARLRRR
                     YLGFVFQGFNLLARTSAQENVELPLLYRGESAQVRHAAAAKALQSVGLGGWEHHTPAE
                     LSGGQQQRVAIARAIVTEPAVVLADEPTGNLDTQRSKEIMELLRALNVDHGITVLMVT
                     HEADMAAYARRVVHFVDGRIDSDTANQRLASSDHPAASAGNP"
     gene            5631..6845
                     /locus_tag="AC16HB_12455"
     CDS             5631..6845
                     /locus_tag="AC16HB_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607644.1"
                     /translation="MLLNALFLALRSIRRNLLRSFLTILGIVIGVSAVITMVTLGHGA
                     TQAVQMQISSLGTNLLMVRPGQRQGPGGPGGGGGAPSFKEADADAILAQIGGVAAVAP
                     EGRIGVTVIGNGRNWATSVTGSSNSWFDTGNWKLASGRKFTVEELQAGAAVCIVGETV
                     RREIYGGTRGVGEQLRIKQFSCDVIGVLASKGQGAMGNDQDDTVLLPLHTLQRRVTGN
                     QRINTLLVSMEDGSDSTRLKASLRQLLRERRKLAAGDDDNFNVLDTQQLADTLSGTTQ
                     VMTTLLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGLRLAIGALEREVLLQFLIEAV
                     VLAALGGIVGIVIATAASIVLAGVMSVPYLFDLTINLLSFVFSAGIGVLFGYFPARRA
                     AQMDPIEALRHE"
     gene            7246..8214
                     /locus_tag="AC16HB_12460"
     CDS             7246..8214
                     /locus_tag="AC16HB_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5607645.1"
                     /translation="MQFKDYYETLGVPRDATAEQIKKAFRSLARKYHPDVSKEPNAEL
                     RMRDINEANAVLSDPEKRSAYDALAQGHRPGQEFQPPPDWDAGFEFSGPGHTAAEAAE
                     FSDFFAELFGRMGAARHGRDQDVHGGGAGFEARGQDHHAKILLDVEDAWRGARQQISL
                     RAPRLDAQGRVVLDTRTLDVQIPKGVREGQLIRLAGQGSPGLGKGAPGDLFLEVHFRT
                     HARFAVSGRDLIVELRVAPWEAALGAVVPVELPDGSTLKVRIPAGAQSGRQLVVRGKG
                     IPGEPPGDLELNLRVVLPPADMPRARELYEAMARDLEFDPRATTGG"
     gene            8220..8528
                     /locus_tag="AC16HB_12465"
     CDS             8220..8528
                     /locus_tag="AC16HB_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HIR5607646.1"
                     /translation="MRDEDILMGALLEDACLTLDQLASACGVPQDWVARRVHEGLLST
                     PGVTPSEWRFSSRELRRVRAMRRIERDFDAVPELAALMADLLEEVEELRTQLRRAGFS
                     "
     gene            9058..10752
                     /locus_tag="AC16HB_12470"
     CDS             9058..10752
                     /locus_tag="AC16HB_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5607647.1"
                     /translation="MNRPWLAQYPAGVPAEIDIRTFASLKDVLAASCARFADRPAFNS
                     MGTAMTFRQLDGASRAFAAWLQKVAGLKRGDRVALMLPNLLQYPVAMFGALRAGMVVV
                     NVNPLYTPRELEHQLNDAGATAIVVLENFAHTLEQVIQRTPVRIVITTQVGDLLPPLK
                     RLLTNVVVKYVKKMVPPWRLDGAISFNRVLAAGRAQSPDDVLLTHDDLAFLQYTGGTT
                     GVAKGAMLTHGNMVANVLQVAAWMSPNLKDGTETVVLPLPLYHVFALTGCLSFFSKGA
                     QAVLIANPRDLPVFLKTLRQQPFSALIGVNTLFRALLDAPESATVDWSRLKLTVAGGM
                     AVQHVVAHRWKQMTGVPLVEGYGLTESAPVAIANPVTIEEWSGQIGVPLPSTDAAILD
                     DDGKPVALGEVGEICLRGPQVMKGYWNRPEETANVFTAGGWLRTGDMGVMDERGNIRI
                     TDRKKDMIVVSGFKVFPNEIEDVLTTHPGVAEAAAIGVSDARSGEAVKVVVVLKDPAV
                     TEAALIAHCKQHLTAYKMPRIVEFRSEPLPKSNIGKILRRELRDDRAKASAAAAQT"
     gene            10769..11296
                     /locus_tag="AC16HB_12475"
     CDS             10769..11296
                     /locus_tag="AC16HB_12475"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607648.1"
                     /translation="MTAKYRLRRLRLRDGRAVTLRAVVAGDAAEIVQAFERLSAASRY
                     SRFMQHKKQLTPAALERGVHPRPGRDFTLVATVPAADGIDIVGAAQYVRADESDTKNC
                     EFAITVAEDWRHSGLATRLLDSLVRRARHDGYRNMKGSVIAGNVSMISLARTLKFDVM
                     LEPDDATLVRVQRAL"
     gene            complement(11593..13461)
                     /locus_tag="AC16HB_12480"
     CDS             complement(11593..13461)
                     /locus_tag="AC16HB_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5607649.1"
                     /translation="MRLLLINPRFTESFWSFRFAMTEIVPGKRTVNPPLGLATLAALC
                     PPHWQVTIVDENVEAVPLAPEVDIVGVCGMGVQFPRQSELLAYYRSRGYYVVAGGSYA
                     SLCPEEYERVADTTIAGEAEYIWPAFCRDVESAAPKRLYRETGTVALADSPTPRFDLL
                     KLDRYNTASLQFSRGCPYLCDFCDIIVMFGRRPRMKSVEQIGRELDALRAQDARQVFF
                     VDDNLIGNRAQAKTLLRFLADYQKHHDYRFAFGTEASLNLAQDDELMMLMREAHFAWV
                     FIGIETPDEETLKASRKTQNMGTDILGALQRIYAHGIDVLGGFIVGFDNDTLETFERQ
                     RRFILDSGIQAAMVGLLTALPRTPLYERLRREGRLLERADNGDNTRLGTNIMPKRMTY
                     EAMISAYRRLYEQLLTDRGIAERIRNKLRWMRAPVYRSEYTAVDKVRILIHLLAKGIL
                     PGGPWRWAAFVRTLPVLNPRQVPTVISDWITGLSMQSYVRRRFIRDHAEGTAVERRVR
                     ALRRVVARYLDAGKVALAWRPGPLQLDLTIAGLLDARFFSRTAPRLEKLLRHTHSTLT
                     LHIEELHAQHVAHLQRLLARLARHGDRVSVVLHEKLRTVVPVDSSVFNLVLWTRRV"
     gene            complement(13821..14123)
                     /locus_tag="AC16HB_12485"
     CDS             complement(13821..14123)
                     /locus_tag="AC16HB_12485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5607650.1"
                     /translation="MTVHKAHLVRLEAKPGKEAVLRDLLTGAVALANQEARTTVWFAL
                     EIGPSTFGVFDAFDDEEGRQAHLNGAIAQTLMARAPELLTRPPQIDPVDVLGAKLP"
BASE COUNT         2476 a         4666 c         4729 g         2298 t
ORIGIN      
        1 ccgagcggta agacctacgc gtcggccgga gtgcaatgcg ctctcaatcc gatctcaggc
       61 atgggcccga tgattcaagc ggggctatac aacccgaaga agagcgcgag cgccgctgtg
      121 tcgctcaacg gcaccccggc ggcaacggtc acattcctga gtccggacgc tgcggtgtgg
      181 ctcaccaacg ggatgaacac ggtggttgtg gcgctggaca agaagacagc cgacagctat
      241 tcgttcgatg cgtcgtcgac acaacagaat gtctgcattc cggacaccag tggcaatacg
      301 gtgagcgggg acctggaact cgcaggcagt ggtaagtcct acgcaacggt gacgcccggt
      361 tgtgccttga attcgctcac gggtcgcgcg caaccctacg tgaatttctt cgacaacggc
      421 gcctacctgc tgaatgtctc cgtcaacaac gtgccgctga cgcagttgaa tggcacgacc
      481 cggaggagca cgcccgtctt tctaagcgct gggctgaacg tcctctcggc cgccaacggt
      541 tccatttcca ccgactacta cgtccgcgac ggcggcacag ggagttgcac gctgccgtaa
      601 ctgaggttgc ccccctttcg cggacacctc attccgttgg aagaaacaat cggaacatgc
      661 cgacatccaa gccctcgtat ccggtcgaat ctcgcccgca gaggatcgag ccggtgcggg
      721 ccggcatccg gacaccgtcg aattcgcgtc ggtggtgacg caaggccggc gatgttcgat
      781 cgctccggcg gcatcggcgc tgtagggctc gccgcaggaa cgggggatgt gcgctgccga
      841 gcgagacgca ctccctcgcg cttgccgtcg cgccgactca gtgtcgccgg ggctcgcccc
      901 gttgccagct cggcagcgtg tagccggtgt acagcgccag tccgcgcagc gctgtcgccg
      961 ccgcgcttgc cgcaaccagc gcggtccatt ccgacgcgcc gaactgctgg gcgaacacga
     1021 gcacccatcc gccgatgaag gagcacaccg cgtacgggcg gtgatcgcga aacgcgctcg
     1081 ggatctcgtt gcagacgatg tcgcgcagca cgccgccgaa caccgcggtg accatgccca
     1141 tcagcaccgc gacaatcgac ggcagcatcg cctcgagggc gatctgcgta ccgctggcgg
     1201 tgaacagtcc cagtccgagt gcatccggcc attgcatcgc gcgctcggtc ggctcgaaat
     1261 agcgggcgcg gaggaagatc atcgcgccga tggccagcgc gatgatggcc cacagccagg
     1321 cggcatgtgc cacccaaaag aacgggcggc gatcgagcag aatgtcgcgc agcgtgccgc
     1381 cgccgaaggc ggcaagcccg gtgacaacgc acacgccgac cgcgtcgagg tgcttgcgcg
     1441 ccgccgccaa taggcccgac agcgcgaacg ccacgatggc gaccgcctcg atggcaaact
     1501 gggcgttcga cagcgtccac agtgaagcgc tcggcatggc gacctcgtcg gcaaaggtgg
     1561 cgcattatcg tcgtcgcgcc ggcacgcggt ggctgcgcct aggcaatgcg gccgcgatgt
     1621 ctcgaccaga cgcttcgcaa actcggcccg ccctttcatc ttcgcgtgtg gatcggcctt
     1681 gatgcgtgca acttcgatcg cctcgaggat ctgcttgatg gttggcgcca atcatcctga
     1741 ccatcggcaa ggccgctgct gccgccgagt tgcattcaga gaagtgcgcc gtgggcaatg
     1801 tgcgctatag aacagacgga cactgaagcg gccgcgcatc atgcaagcgg ccctcgtacc
     1861 gggtgggtga caccgagacc ccgcgcctga cgccaattga ccgctcgcaa acaccacgcc
     1921 gcaaggcgcc gtcacacttg ctctgatgtt gcctgatgct cgcctgagcc gtgtcgacct
     1981 ttcacgctaa cgcctgcgtg acccgtacgc gtgggatgcg cagcttgatg gccgtcggct
     2041 gtctgttgct ggccctgaac ggttgcgctt cggtggcgcc gcagagtgcc ccattcgccg
     2101 cggtcgatat tccggccgca tggtcggccg ccgatccttc cgcgacgacc gacacatcct
     2161 cgctttcgca gtggtggttg cgcttcgacg atcccctact cggcaacctc gtgaaccagg
     2221 ctttgcaggc caatacgact gtcaagagcg cgcaggccgc gctgcggcag gcgcgggccc
     2281 tgcgtgatgt gtcggcggcc ggtctgttgc cagcggtcgg cagttcggca tctgcgcagc
     2341 gcagcacctc aggcagcaac gatgcaacca acaatttcag ggtcggtctg gacgcgagct
     2401 gggaactcga catcttcggc gccaaccgca gtgcgctgga cgccagcaac gccgcggtac
     2461 aggccagcgc cgcaagcttg ggcgacgtgc aggtgtcgat cgccgccgaa gtggcgctcg
     2521 gctacatcac gctgcgcggc gcgcaagcgc ggctggcgat tgccgacgag aatctggcca
     2581 gccagcttga aacactgcag atcacgcagt ggcgattgca ggctggcctc gtcacttcgc
     2641 tcgaagccga gcaggcgcgt gcggcggccg aacagacacg cgcacagttg ccggcgttgc
     2701 agacgagcat cgcgcagacc cgtcatgcgc tcgcggtgct gaccggccag ccgccggcgg
     2761 cattggcgac cttgctggcg acaatgagtc cggtgccgca ggcggctgac ggcctggtgc
     2821 tgagcatccc cgccgagaca ctgcgccagc ggcccgatgt gcgtgccgcc gaacagcaag
     2881 tggcggcagc gatcgctcgc gttgcgcaag ccgacgcggc gcgcatgccg aacttcaagc
     2941 tcggcggttc gctgggcctg aacgcattga cgctcggctc gctgaccaac ggcgcgtcgg
     3001 tcgtcagttc gctgctggcc agcgtgtcga tgccggtgtt cgacggcggt gccctgcgcg
     3061 cccaagtgcg cgcgcagcag gccgcgctgg ctcaagcggg cgcggcatac caggccacgg
     3121 tgctgaccgc attgaaggat gtggaagatg cattgatcgc gctgcgcggc gaccgcgaac
     3181 gcctgctgcg attgcagcac gccgccgaag cagccagcaa cgccgcgctg ctggcgcgcc
     3241 agcgtttcag cagtggcttg gtggactttc agactgtgct cgaaacccaa cgcacgcaac
     3301 tgaacacgca ggacagcgtg gccagtgccg gcgcagacct cagcgccgac catgtgcgcc
     3361 tgtacaaggc gctcggtggc ggctggcgcc ccgaagacag cgacgcgacg ccctcctcca
     3421 ccgaagacgc accccggatt ccccgttcat gaacacgccc aacccttccg ccacggtcgc
     3481 gcctgcaccc gaggtcagca cagaaaccga cctcgcgacg ttgctcgagg agccctcgtc
     3541 gcgcgcctgg tatcgccgcc cggcagtgtg ggccggcgtg ctgctgttgg cggtggtggc
     3601 ggcgggcctg tggtactggc aagcgcgcca gaccgccagc gcagcgccga gttacacgac
     3661 gcacaccgtc gcgcgcggca atctcacgct cagcgtcacc gcgaacggca cgctgcagcc
     3721 gacacgctcg atcagcatcg gcagcgaact ctcgggcacc gtgctgaagg tcaacgtcga
     3781 cgtcaatgac cgcatcagga agggccaggt actggtcgaa ctcgatactt ccaagttgcg
     3841 cgaccagatc ctgcgctcgc gcgcatcgct cgcggccgcg aacgcgaagg tcgctcagac
     3901 cgtggcgacg gtgaaggagg cgaaggcgag cctggcacgg ttcgaggaag tggcacggct
     3961 ttcaggcggc aaggtgccgt ccaaggccga gctcgacagc gcgcgagcgg cactcgaccg
     4021 tgcgatcgcc gacgaggcca gcgcccgcgc cagtgtggcc gacgcgcagg cggcactgtc
     4081 aaccgacgag atcaacctct cgaaggcgtc gatcagcgcc ccctccgacg gcgtggtgct
     4141 gacgcgcagc gtggaccccg gcaatgcagt ggcggcatcg ctgcaggcgg tcacgctgtt
     4201 cacggtggcc gaggacctgc agaaattgcg cctatgggtc tatgtcgacg aggccgacgt
     4261 aggctcagtg gcggcaggtc aggaggcgac cttcaccgtc agcgcctatc cgagccgcaa
     4321 gttcccggcg cgcgtcactc gcgtgggctt tgggtcgacc atcacagaca acgtcgttac
     4381 ctacctgacc tacctggacg tggacaacag cgacctgagc ctgcgtcccg gcatgacggc
     4441 caccgcgacg atcatcgcga cgcagcgcac cgacgtgctg ctcgtgccca acaccgcgct
     4501 gcgcttcgcg ccgacggcag cgggcgccgg agcaccggcg gccaagggcg gcatcgtttc
     4561 caccctgatt ccgcgcatgc ccagaagcag cacgcgaact tcggccgcgg ccggcgcgag
     4621 caccgctgcg gcgcgcgagg tctgggtatt gcgcgacggt gctgccgtgc cggtcgcggt
     4681 gatcccaggc atcagcgacg gccgcatgac cgagatcacc ggcggcgact tgcaagccgg
     4741 catgcaggtg atcaccgacc agaagacggt aaccgcgaag tgagcgcagt gctgcccatc
     4801 gaaacgcttg tgcccgagcc gacgggcact gccggcgccc cgcatgacat gccgcctctc
     4861 atcaacgcac cggccgccgc acctccggtg aaagccggca cgccgctgat ccgcctgcgc
     4921 ggcgtcacca agagatacgg taccggccag gccgagctga tggcgttgaa aggcatcgac
     4981 ctcgacatca cggctggcga gttcgtcgcg atcatggggc ccagcggttc gggcaagtcg
     5041 accgcgatga acatcctggg ttgcctcgac acgcccagcg ccgggcagta cctgttccat
     5101 ggcgcgcatg tcgaagcgct gtcgcgcgac cagcgcgcgc ggctgcgccg ccggtacctg
     5161 ggcttcgtgt ttcagggctt caaccttctg gcccgcacct cggcgcagga gaacgtcgaa
     5221 ctgccgctgc tgtaccgtgg agaaagcgcg caggtgcggc acgcggccgc ggccaaggcg
     5281 ctgcagtcgg tcgggttggg cggctgggaa caccacacac cggccgagtt gtcgggcggg
     5341 cagcagcagc gggtggctat cgccagggcc atcgtcaccg agccggccgt cgtgctggcc
     5401 gacgagccca ccggcaatct cgacacccag cgcagcaagg agatcatgga gctgctgcgg
     5461 gccctgaacg tggatcacgg catcaccgtg ctgatggtta cgcacgaggc cgacatggcg
     5521 gcctatgcac ggcgcgtggt gcacttcgtc gacggccgaa tcgatagcga tacggccaac
     5581 cagcgccttg cctcctcgga ccatccagca gcctcggcag gcaatccctg atgcttctca
     5641 acgcgctgtt tctcgcactg cgttcgatcc ggcgcaacct gttgcgctcg ttcctgacca
     5701 tcctgggcat cgtgatcggc gtcagcgcgg tcatcacgat ggttacgttg gggcacggcg
     5761 ccacacaggc cgtgcagatg cagatctcca gcctcggcac caacttgctg atggtccgcc
     5821 cgggccagcg ccaggggccc ggtggccccg gcggcggggg aggtgcaccg tcgttcaagg
     5881 aggccgatgc cgatgcgatc ctggcgcaga tcggcggcgt cgccgcggtg gcgcccgagg
     5941 gccgcatcgg tgtcaccgtg atcggcaacg gccgcaactg ggccaccagc gtgacgggca
     6001 gcagcaatag ctggttcgac accggcaact ggaaactcgc ctcgggccgc aagttcacgg
     6061 tcgaggagtt gcaggccggc gccgccgtct gcatcgtcgg cgagacggtg cgccgcgaga
     6121 tctacggcgg cacccgcggc gtgggcgagc aactgcgcat caagcagttc tcgtgcgacg
     6181 tgatcggcgt actcgcgtcc aaaggccaag gcgcgatggg caacgaccag gacgacacgg
     6241 tgctgctgcc attgcacacc ttgcagcgcc gcgtcaccgg caaccagaga atcaacacgt
     6301 tgctggtgtc gatggaggac ggcagcgaca gcacgcggct gaaggccagc ctgcggcaac
     6361 tgctgcgcga gcgccgcaag ctcgccgcag gcgacgacga caacttcaat gtgctcgaca
     6421 cgcagcagct tgccgatacg ctatcgggca cgacgcaggt gatgaccacg ctcctcggcg
     6481 ccgtggccgc ggtgagcctg ctggtgggcg gcatcggcat catgaatatc atgctggtga
     6541 gcgtgacgga acgcactcgc gagatcggcc tgcgcctggc catcggcgcg ctcgagcgcg
     6601 aggtgctgct gcagttcctg atcgaggcgg tggtcttggc ggcgctgggc ggcatcgtcg
     6661 gcatcgtcat cgccaccgcg gcgtccattg tgctggccgg cgtgatgagc gtgccctacc
     6721 tgttcgatct gacgatcaac ctgctgtcct tcgtgttttc ggccggtatc ggcgtgctgt
     6781 tcggttactt cccagccaga cgcgccgcgc agatggaccc gatcgaggcg ctgcggcacg
     6841 aatagagccc gcaccctgtt cggagcccgg atgcaagccg cagaattgtg cgcggtcaat
     6901 gtgtgtcgca cagcacacca cgccagagct gaagttggtc acgggtggaa acgcgaatcg
     6961 cagaaccgcg cgtcggtcac tcggcactca gaggttctcg atgaatctcg ggcttgtctc
     7021 gaacaggact tcggatgcga taagtgacac ggctgcgccg agccgcagtg aatcgaagct
     7081 gccgccagcg ctcgggtcgg ccggctcatg tgaactcttg gttcgtgttc gccccgtgcc
     7141 gatttcaagg gcttgatcgc ggcctgttga tcgatatcta taacgaggct cgccaacgaa
     7201 cgtaacggct ggaatgatcc ggccggttcc aggaagaggt catccgtgca attcaaggac
     7261 tactacgaga cgctgggcgt gccgcgggat gcgaccgccg agcagatcaa gaaggcgttc
     7321 cgcagcctcg cgcgcaagta ccaccccgac gtcagcaagg agcccaacgc cgagctgcgg
     7381 atgagggaca tcaacgaggc caacgcggtg ctctcggacc cggagaaacg ctcagcctat
     7441 gacgcgctcg cgcagggcca ccggccgggc caggagttcc agccgccgcc ggactgggac
     7501 gccggattcg agttctccgg gcccgggcac acggccgccg aggccgccga attcagcgat
     7561 ttctttgccg agttgttcgg ccgcatgggc gcggcacggc acggccgcga ccaagacgtg
     7621 catggcggcg gtgccggctt cgaagcgcgc ggacaggacc accacgccaa gatcctgctc
     7681 gacgttgaag acgcctggcg cggcgcgcgg cagcagatca gcctgcgcgc gccaagactg
     7741 gatgcacaag gtcgagtcgt gctcgatacg cgcacgctcg atgtgcagat ccccaagggc
     7801 gtgcgcgaag gccagttgat ccggctggcc ggccagggct caccgggtct cggcaagggt
     7861 gcgcccggcg atctgtttct cgaagtgcat ttcagaacac acgcgcgctt cgccgtatcc
     7921 ggccgcgacc tgatcgtcga gctgcgcgtg gcgccttggg aggccgcgct cggtgccgtg
     7981 gtgccggtcg agctgccgga cggcagcaca ctgaaggtcc gcatccccgc cggcgcacag
     8041 agcggccggc aactggtcgt tcgcggcaaa ggcattccgg gcgaaccgcc gggcgatctc
     8101 gagctgaacc tgcgcgtggt gctgccgccg gcggacatgc cgcgcgcccg cgagttgtac
     8161 gaggcgatgg cgcgtgatct cgagttcgat ccgcgcgcga caaccggagg ctgacggcga
     8221 tgcgagacga agacatcctg atgggcgcgc tgctcgaaga cgcgtgcctc acgctcgatc
     8281 aacttgccag cgcctgtggc gtgccacagg actgggtcgc ccgccgcgtc cacgagggcc
     8341 tgctgtcgac tccgggcgtc acgccgtccg agtggcgctt cagcagccgc gagctacgcc
     8401 gcgtgcgcgc catgcgccgc atcgagcgcg atttcgatgc cgtgcccgaa ctcgccgcgc
     8461 tgatggcgga cttgttggaa gaggtcgagg aactgcgaac acagttgcgc cgcgcaggat
     8521 tttcctgagc gcagcggaca tgcccccgcc tcaggcgcgg gcgagcgcac cgagcgacgc
     8581 gcggcttcgt aatgtcgtcg actgaacagt tgcgtctcgg tcgcgggcac gtcggcgttg
     8641 gctctagcgc taacagacgc ggcgcgcacc gctgttgcca cttcagcgtg ccggatcgcc
     8701 ggtactggag gcggccccga tgcagcggaa tatgcgcatg ccaaaccgtt ggtggcgttg
     8761 cggtggcggt ggcaaaccgt gattgcgcct gctgtccgcc gaggttcgcc ggcctgctcc
     8821 aaaccttcat gcgggtgctc ccggaagggg tgacaaagca tctgctgccc gccgccgatt
     8881 cactgcgacg actccgcacg ggatgtcccc gttcgtctgg acgggaatcc taaccctccg
     8941 aggtgctcgg cagcggccat tgcgcacttc cgttgtcatc gcgcagtacg cggcgccaca
     9001 cggcggtcag actgcatacg agtacaagcc tgatccacaa cagggcgcga ggagcacatg
     9061 aataggcctt ggctcgccca gtacccggcc ggcgttccgg ccgaaatcga catccggacg
     9121 ttcgcgtcgc tgaaggacgt tctggccgcg agttgcgcgc gttttgccga ccggcctgca
     9181 ttcaactcga tgggcacggc gatgacgttc cggcaactcg acggggcgag ccgcgccttc
     9241 gcggcctggc tgcagaaagt ggccggtctg aagcgtggcg atcgcgtcgc cctgatgttg
     9301 cccaatctgt tgcagtaccc ggttgcgatg ttcggtgccc tgcgcgccgg catggtcgtg
     9361 gtgaacgtca atccgctcta cacgccgcgg gaactcgagc accaactcaa cgatgcgggc
     9421 gccaccgcca tcgtggtcct ggagaacttc gcgcacaccc ttgagcaggt gatccagcgc
     9481 acgccggtgc gcatcgtgat cacgacgcag gtgggcgacc tgctgccgcc actgaagcgc
     9541 ctgctgacca acgtcgtcgt caagtatgtg aagaagatgg tgccgccgtg gcgactggac
     9601 ggtgcaataa gtttcaaccg cgtcctcgct gccggccgcg cgcagtcgcc tgacgacgtg
     9661 ctgctcaccc acgacgatct cgccttcctg cagtacaccg gtggcacgac cggcgtggcc
     9721 aagggtgcga tgctcacgca cggcaacatg gtcgccaacg tgcttcaggt cgccgcgtgg
     9781 atgtcaccca acctgaagga cggcacggag accgtggtcc tgccgctgcc gctgtatcac
     9841 gtgtttgccc tgacaggctg cttgtccttc ttcagcaagg gggcgcaggc cgtgctgatc
     9901 gcgaacccgc gcgacctgcc ggtgtttctc aagacgctca ggcagcagcc cttctcggcg
     9961 ctcatcggcg tcaacacgct gttccgcgcg ctgctcgatg cgcccgaatc cgccacggtc
    10021 gactggagtc gcctgaagct cacggtcgcc ggcggcatgg ccgtgcagca tgtcgtcgcg
    10081 catcgctgga agcagatgac cggcgtgccg ctggtggaag gctacgggct caccgagagc
    10141 gcgccggtgg cgatcgccaa tccggtcacc atcgaggagt ggagcggtca gatcggcgtg
    10201 ccgctcccaa gcacggacgc ggcgatcctc gatgacgatg gcaagccggt cgcgcttggc
    10261 gaagtgggcg agatctgcct gcgcggaccg caggtgatga agggctactg gaaccggccc
    10321 gaggagaccg ccaacgtgtt caccgccggc ggctggctgc gcaccggcga catgggcgta
    10381 atggacgagc gcggcaacat ccgcatcacc gaccgcaaga aggacatgat cgtggtgtcc
    10441 ggattcaagg tgttccccaa tgagatcgag gatgtgctga cgacgcaccc cggcgtggca
    10501 gaggcagcag cgatcggcgt gtccgatgcg cgttcgggcg aggcagtcaa ggtggtggtc
    10561 gtgctcaagg acccggcggt gacggaagcc gcactgatcg cgcactgcaa gcagcatctg
    10621 accgcctaca agatgccgcg catcgtcgag ttccgcagcg agccgctgcc gaagtccaac
    10681 atcggcaaga tcctgcgcag agagttgcgc gacgaccggg cgaaggcgtc cgcggcggcc
    10741 gcccaaacat agtggggata ccgcaccgat gacagcgaaa tatcgtctgc gccggctcag
    10801 gctgcgcgac ggccgtgcag tgaccctgcg cgcagtcgtc gcgggcgatg cggcggagat
    10861 cgttcaggcg ttcgagcgtc tgtcagccgc gtcgcgttac agccgattca tgcaacacaa
    10921 gaaacagctc actcccgcgg cacttgagcg cggcgtgcac ccacgtcccg gccgtgactt
    10981 cacgctggtg gccaccgtgc cggcggccga cggcatcgac attgtcggtg ccgcgcagta
    11041 cgtgcgcgcg gacgaaagcg atacgaagaa ctgcgagttc gcgatcaccg tggccgaaga
    11101 ctggcgtcat agcggcctcg ccaccaggct gctggacagc ctggtgcgcc gagcgcggca
    11161 cgatggctac cgaaacatga aaggctcggt gatcgccggc aacgtgtcga tgatttcgct
    11221 ggcgcgaacg ctgaaattcg acgtgatgct ggagccggac gatgcaacgc tggttcgcgt
    11281 gcagcgcgcg ctgtagatga catctcgctc cctgggcgga gtcctgaacg cgggccgtgt
    11341 tgagcaactg atccatttca tctgagcgca agtacccgcg tttgagatcc cacccgcgcg
    11401 acggcagctc gaaccgttgc caatccggag ctcttgtcgc acgaggcgct accctggttt
    11461 ttcctgctgt tcgaatcgcc cgaaagcggc tcgtcacgaa cgcctggcac tcgctgcatc
    11521 tggacagcga cgcctcatct cgagcggctg ctcgccgtgt tgggatcggc cggaccacga
    11581 tgaacgccgc gctcacacgc gccgcgtcca cagcaccaga ttgaacacag atgagtcgac
    11641 cggcacgacc gtgcgcagct tctcgtgcaa gaccactgag actcgatcgc cgtggcgcgc
    11701 aagccgcgcg agcagccgct gcagatgagc cacgtgctgc gcatgcagct cctcgatgtg
    11761 cagggtgagc gttgaatgcg tgtggcgcag caacttctcc agccgcggcg cggtgcgcga
    11821 aaagaaacgc gcgtcgagta agccggcgat tgtcaggtcg agctgcagcg ggccgggccg
    11881 ccaagcgagt gccaccttgc ctgcatccag atagcgcgcc accacgcggc gcagcgcgcg
    11941 cacgcgtcgt tctaccgccg ttccttctgc gtgatcgcga atgaaacgcc ggcgcacgta
    12001 ggactgcatt gacaagccag tgatccagtc ggagatcacc gtcggcacct ggcgtggatt
    12061 caggaccggc agcgtgcgga cgaacgccgc ccagcgccag ggaccgccgg ggaggattcc
    12121 cttcgcgagc aggtgtatga ggatgcgcac cttgtcgacc gcggtgtact cgctgcggta
    12181 caccggcgcg cgcatccagc gcagcttgtt gcgaatgcgc tcggcgatgc cgcggtcggt
    12241 cagcagttgc tcatatagcc gccggtaggc ggagatcatc gcttcgtaag tcatgcgctt
    12301 gggcatgatg ttggtgccca ggcgcgtgtt gtcgccgttg tcggcacgct cgagcaggcg
    12361 gccttcccgt cgaaggcgtt catacagcgg cgtacggggc agcgcggtga gcaaccccac
    12421 catcgccgcc tggatgccgg agtccaggat gaagcggcgc tggcgctcga aggtttcgag
    12481 cgtgtcgttg tcgaagccga cgatgaaccc gcccagcacg tcgatgccgt gcgcgtagat
    12541 gcgttgcagc gcgcccagga tatcggtgcc catgttctgc gtcttgcggc tcgccttgag
    12601 cgtttcctca tccggtgtct cgatgccgat gaagacccac gcgaagtgcg cctcgcgcat
    12661 cagcatcatc aattcatcgt cctgcgcgag gttcagcgac gcctcggtgc cgaaggcaaa
    12721 gcggtagtcg tggtgcttct ggtagtccgc cagaaagcgc agcagcgtct tggcttgcgc
    12781 gcgattgccg atcaggttgt cgtcgacgaa gaagacctgc cgcgcgtcct gcgcacgcag
    12841 cgcgtccagt tcgcggccga tctgctccac gctcttcatg cgcgggcgcc ggccgaacat
    12901 cacgatgatg tcgcagaagt cgcacaggta cgggcagccg cgcgagaact gcaggctcgc
    12961 ggtgttgtag cggtcgagtt tcagcagatc gaagcgcggc gtgggcgagt cggcgagcgc
    13021 aacggtgccg gtctcgcggt acaggcgttt gggcgcagcg gactcaacgt cgcgacagaa
    13081 cgcgggccag atgtattccg cctcgcccgc aatcgtggtg tcggcgactc gctcgtactc
    13141 ttccggacac agcgacgcgt agctgccgcc ggccaccacg tagtagccgc ggctgcggta
    13201 ataggccaac agttcgctct ggcgcggaaa ctgcacgccc atgccgcaca cgccgacgat
    13261 gtctacttct ggggcaagcg gcaccgcttc cacattttcg tccacgatgg tcacctgcca
    13321 atgcggcggg cacagtgccg ccagcgttgc gagtcccagc ggcggattga ccgtgcgctt
    13381 gccgggcacg atctcagtca tcgcgaagcg aaagctccag aagctttccg tgaagcgcgg
    13441 attgatgagc agcagtcgca tgcagcggtc cgcatggtgt ggttgaggaa cgcttcgacg
    13501 cgacagcgcg gaagcgacca tcactgcggt cggcgaagcg gcttcgagga agcgttcggg
    13561 cccatgcgtg gcttgaaacg ttggcgcacg aatcagccgc gcgcaagggt gctccccggc
    13621 caggtgcgga cttgttaaca aggatcaaaa gatcggcggc gcatatccgt taggcagcgt
    13681 caggttgcgg ttcatcaaat tcggcttctc gcccgtgttc gcattgaagg tcaggagtcg
    13741 agcgcgaacg aaggaccgag cctcgacgcg aaccacacga tggtacgcgt cgaggtatct
    13801 gcagttcgaa tcctctgtcg ctatgggagc ttcgccccca gcacatcgac cgggtcgatc
    13861 tgcggcggcc tggtcagcag ctcgggcgcc cgggccatca gcgtctgcgc gatcgcgccg
    13921 ttgaggtggg cctggcggcc ttcctcgtcg tcgaaggcgt cgaacacgcc gaaggtcgac
    13981 ggtccgatct ccagcgcgaa ccacaccgtg gtgcgcgctt cctggttggc cagcgccacg
    14041 gcgccggtta gcaggtcgcg taacacggct tccttgcccg gcttggcctc gaggcggacc
    14101 aggtgtgcct tgtgaaccgt catgtcattt ctcctttggt aatcatcgaa cccgacattg
    14161 ggtgaggcg
//
LOCUS       DBNHBY010000510         1757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_778897, whole genome shotgun sequence.
ACCESSION   DBNHBY010000510 DBNHBY010000000
VERSION     DBNHBY010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_778897"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16HB_12490"
     CDS             <1..277
                     /locus_tag="AC16HB_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase family protein"
                     /protein_id="HIR5607651.1"
                     /translation="AHLAVARRNDAVLTLSWPLQEFYNSHWAPPDAIVARPAGSVVEV
                     DMTPVDASASEIRALLAEPRSPARDARLAAIVPAAVLDYIHEHALYA"
     gene            282..653
                     /gene="rsfS"
                     /locus_tag="AC16HB_12495"
                     /pseudo
     CDS             282..653
                     /gene="rsfS"
                     /locus_tag="AC16HB_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
     gene            714..1181
                     /gene="rlmH"
                     /locus_tag="AC16HB_12500"
     CDS             714..1181
                     /gene="rlmH"
                     /locus_tag="AC16HB_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR5607652.1"
                     /translation="MKITVVAVGQRQPHWADEAIEEFVKRFPPGFAVTINEVKAETRS
                     GQPVERILAAEAERLRAAVPPGAVVVALDERGQDWTTARFAEQLGSWRDASESLAFLI
                     GGPDGIAPELKRSARISLRLSSMTLPHALARLLLVEQLYRAWSILANHPYHRA"
     gene            1186..>1757
                     /locus_tag="AC16HB_12505"
     CDS             1186..>1757
                     /locus_tag="AC16HB_12505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR5607653.1"
                     /translation="MTDLYLASRSARRRELLKQIGVRFEPLLLRLAQPRGPDIDELQH
                     ADESAAAYVDRVAREKAEFGLRVLGMRSMLLKPVLAADTIVIVDGEVLGKPANPTEAA
                     AALRRLSGRVHEVRTSVALAFGGPNSPRVNVVTSATQVRMRAIDDDEIKRYCASGEPH
                     DKAGGYAIQGLAAVFIEHIEGSYSGVMGLPL"
BASE COUNT          289 a          568 c          626 g          274 t
ORIGIN      
        1 cgcgcacctg gcagtcgcgc gccggaacga cgcggtgctc acgctttcgt ggccgctgca
       61 ggagttctac aactcgcact gggcgccgcc cgacgcgatc gtggcgcgcc cggccggatc
      121 ggtcgtcgag gtcgacatga cgcccgtcga tgcgtcggcg agcgagatcc gcgcactgct
      181 tgccgaaccg aggtcgccag cgcgcgacgc acgattggcc gcgatcgtgc cggcggcggt
      241 gctcgactat attcacgagc atgctctcta cgcgtaggtc aatggacatc cgcaaactgc
      301 agcgcgtggt cgtcgacgcg ctggaggacg tgaaggcgca ggacgtcgtc gtactgaaca
      361 cgaccaagct gtcggacctg ttcgaccgcg tgatcgttgc cagcggcaca tcgaaccggc
      421 agacccgcgc gctggccgcg agcgtggtgg ataaggtcaa ggccgccggt ggccaggtga
      481 tcagcgtcga agggaccgaa aacggcgaat gggtgctggt cgacctcggc gacgtggtcg
      541 tgcacgtgat gcagccgggg atccgcaagt actacgggct ggaggagatc tggggcggca
      601 agccggtgcg gatgaagctg gcgtccgagc cgcgccggtc ggcggcgacg cggtgatgac
      661 tccatggcgc gcgcattaga ccgagggcgg tatcccgccc gattcgagag acggtgaaaa
      721 tcaccgtggt ggccgtcggc cagcggcagc cgcactgggc cgatgaagcg atcgaggagt
      781 tcgtcaagcg gtttccaccc gggttcgccg tgacgatcaa cgaagtcaaa gccgaaacgc
      841 gcagtggcca gcccgtcgag cgcatcctcg ccgcggaagc ggagcggttg cgcgcggcgg
      901 tcccacccgg cgccgtcgtg gtggcgctcg acgagcgcgg gcaggactgg accacggccc
      961 gcttcgctga acaactggga agctggcgcg atgcttcgga atcgctcgcc tttctgatcg
     1021 gcggccccga cggcattgcg ccggaactca agcgtagtgc gcgcatatcg ctgcggctat
     1081 cgagcatgac gctgccgcat gcgctggcgc ggctgctgct ggtcgagcaa ctctatcgcg
     1141 catggtcgat cctcgccaac catccttatc accgcgcctg aacccgtgac cgatctctac
     1201 ctcgcctcgc gcagcgctcg ccggcgcgaa ctgctcaagc agatcggcgt gcgcttcgag
     1261 ccgctgctgc tgcggctggc gcagccgcgc ggaccggata tcgatgaact gcagcatgcc
     1321 gacgaatcgg ccgccgccta cgtggaccgc gtagcacgcg agaaggccga attcgggctg
     1381 cgcgtgctgg ggatgcggtc gatgctgctg aagccggtgc tcgccgcgga tacgatcgtg
     1441 atcgtcgacg gtgaggttct cggcaagccc gccaatccga ccgaggcggc ggcggcgctg
     1501 cgccgcctgt ccggccgcgt gcatgaagtg cgcacgtcgg tggcgcttgc gttcggcggg
     1561 ccgaattcgc cgcgggtcaa cgtcgtcaca tcggccacgc aggtgcggat gcgcgcgatc
     1621 gacgacgacg agatcaagcg ttactgcgcg tccggcgagc cgcacgacaa ggcgggcggc
     1681 tatgcgattc agggcctggc cgccgtcttc atcgagcaca tcgaaggcag ctactccggc
     1741 gtgatgggtc tgccgct
//
LOCUS       DBNHBY010000511         3763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_913492, whole genome shotgun sequence.
ACCESSION   DBNHBY010000511 DBNHBY010000000
VERSION     DBNHBY010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_913492"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="AC16HB_12510"
     CDS             <1..615
                     /locus_tag="AC16HB_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5607654.1"
                     /translation="KQEGISFLLIDMKSKGVTVRPIELLDGSVEVNEVWFDNVEVPIE
                     QRVGAENQGWTIAKYLLEHERTNIANVGINKREMERAKRLARTLMRNGKPYAEDPLFA
                     ARMAEVEIELMALEILNLRVLSEARARGAPGPMSSMLKIKGAEIIQKLGELVADMQGS
                     AALPARAGRLLPEPAMYLNLRKTTIYGGSNEIQRGIIAKHVLEL"
     gene            618..1631
                     /locus_tag="AC16HB_12515"
     CDS             618..1631
                     /locus_tag="AC16HB_12515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5607655.1"
                     /translation="MNFELTEDQSLLLATCDRWVRERCTIDARRAQAKSDAAVVPATW
                     RELVDLGLASLLVPESNGGHGRLLIDAVLVAQTLGRGWLLEPFIDSAIAAATMLARCA
                     PSPQRDASLAALAEGAIVIPLRDVRAEGTRLSGWAPHASCADVLLALADDRIYEVRAA
                     DCDRRMYRQLDGSAAAAVEFNVGTSVLAEGAAARNAWQAGADAARIGRIAEGIGLAKT
                     VLDLTAEYLRTRKQFGQPIGRFQALAHRMADLLVLYEQAQSLMLAAAMKMDPRTLDAA
                     QVLAHRALRTIAQQSIQLHGGIGMTAEYALSSYVKRMVALEVELGDAETALARFAAGN
                     ARG"
     gene            1780..2355
                     /locus_tag="AC16HB_12520"
     CDS             1780..2355
                     /locus_tag="AC16HB_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tll0287-like domain-containing protein"
                     /protein_id="HIR5607656.1"
                     /translation="MKTSSARGIVSIVLAMAAAGAFADDAKLLEEARGVAMSVPPRLL
                     AVLQEEIAKGGPEGAIEVCRDKAPQLAKEASEKTGWQIRRVSLKNRNPKAVPDAWERA
                     VLQDFDRRAAAGENPATLEKGEVVVDAGTKVYRYMKALPVQPLCLNCHGAAENMSPAT
                     KAKLAERYPGDLGIGCAVGQIRGAMTIKRAM"
     gene            complement(2365..3198)
                     /locus_tag="AC16HB_12525"
     CDS             complement(2365..3198)
                     /locus_tag="AC16HB_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5607657.1"
                     /translation="MHRRRLLLSLVPALIAAAPTTPFAKTSLRVSSVPGGIARIRLGT
                     SELPPRARLGAERVLVMRDGAEWIALVGIALATKPGTKLHVEARRAEGSVQRYEIAVG
                     PKAYATQHLKVAPAQVELSPEDLARYERERAHMAEVLRTFTETPPESLALLQPAPGTR
                     SSSFGLRRYFNGQPRNPHSGMDIAAPEGTPVIAASAGRVIDSGDYFFSGQQIVLDHGE
                     GLLTLYAHLSGIDTHVGDSVSAGTPIGKVGATGRVTGPHLHFSVYLNTVAVDPALFLP
                     A"
     gene            complement(3322..3546)
                     /locus_tag="AC16HB_12530"
     CDS             complement(3322..3546)
                     /locus_tag="AC16HB_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5607658.1"
                     /translation="MPIFEYACQDCGREFETLVRSNTVLQCPQCRSTQLRKRLSVFAT
                     AASAPEAAPAMAGPCGSCGHPDGPGSCALH"
BASE COUNT          637 a         1267 c         1300 g          559 t
ORIGIN      
        1 aagcaggaag gcatcagctt cctgctgatc gacatgaaga gcaagggcgt caccgtgcgg
       61 ccgatcgaac tgctcgacgg ctcggtcgag gtcaatgaag tgtggttcga caacgtcgag
      121 gtgccgatcg agcagcgcgt cggcgcggag aaccagggct ggaccatcgc caagtacctg
      181 ctcgagcacg agcgcaccaa catcgcaaac gtcggcatca acaagcgcga gatggagcgc
      241 gcgaagcggc tggcgcgcac gctcatgcgc aacggcaagc cgtatgccga agacccgctg
      301 ttcgccgccc gcatggccga ggtcgagatc gagctgatgg cgctcgagat cctgaacctg
      361 cgcgtgctgt ccgaggcgcg cgcgcgcggc gcgccggggc cgatgtcgtc gatgctcaag
      421 atcaagggcg cggagatcat ccagaagctc ggcgagctgg tcgccgacat gcagggcagc
      481 gctgcgctgc cggcgcgcgc cggccgcctg ctgcccgaac ccgcgatgta cctgaacctg
      541 cgcaagacga cgatctacgg tggctccaat gaaattcagc ggggcatcat cgcgaagcat
      601 gttctcgagc tgtagtcatg aacttcgaac tgactgaaga ccaatcactg ctgctcgcga
      661 cctgcgaccg ctgggtgcgc gagcgctgca cgatcgacgc gcgccgcgcg caggcgaagt
      721 ccgatgccgc ggtcgtgcct gcgacgtggc gcgaactggt cgatctcggc ctggcgtcgc
      781 tgctggtgcc cgagtccaac ggcgggcacg gccggctgtt gatcgacgcg gtgctcgtcg
      841 cgcagacgct cggccgtggc tggctgctcg agccgttcat cgacagcgcg atcgccgccg
      901 cgaccatgct tgcgcgctgc gccccgtcac cgcagcgcga tgcttcgctg gccgcgctcg
      961 ccgagggcgc gatcgtgatc ccgctgcgcg acgtgcgcgc ggaaggcacg cgcctttccg
     1021 gctgggcgcc gcacgcgagt tgcgctgatg tgctgctggc gttggccgac gaccgcatct
     1081 acgaggtccg cgccgcggac tgcgaccggc gcatgtatcg gcagctcgac ggcagtgccg
     1141 ccgccgcagt cgaattcaac gtcggcacct cggtgctggc cgaaggcgcc gccgcgcgca
     1201 acgcgtggca ggccggcgcg gacgccgcgc gcatcggccg catcgccgag gggatcgggc
     1261 tggcgaagac cgtgctcgac ctcactgccg agtacctgcg cacgcgcaag cagttcggcc
     1321 agccgatcgg ccgcttccag gcgctggcgc accggatggc ggacctgctc gtgctgtacg
     1381 agcaggcgca gtcgctgatg ctcgccgcgg cgatgaagat ggacccgcgc acgctcgacg
     1441 ccgcgcaggt gctggcgcac cgcgcgctgc gcacgatcgc gcaacagtcg atccagctgc
     1501 atggcggcat cggcatgacc gccgagtacg cgctgtcgag ctacgtgaag cgcatggttg
     1561 cgctcgaggt cgagctcggc gacgccgaga ccgcgctcgc ccgctttgcg gccggcaacg
     1621 cgcgcggctg acctcggact ttcccctgag tggccgctgt gacgcgtgtc acagcgtccg
     1681 acctgcgttt tggcaagcat caaggttttc gcggcggagt gcgcgcagca tcttcgtcgg
     1741 atcgccggtt ccttccgacc atggctcagg agtaacgcaa tgaagacctc aagcgcacgc
     1801 gggatcgttt caatcgtcct tgctatggct gcagccggcg cattcgccga cgacgccaag
     1861 ctgctcgagg aggcgcgcgg cgttgccatg agcgtcccgc cgcgcctgct ggcggtgctg
     1921 caggaagaga tcgccaaggg cggccccgag ggcgcgatcg aggtctgccg cgacaaggcg
     1981 ccgcaactgg cgaaggaggc ttcggagaag accggctggc agatccgccg cgtgagcctg
     2041 aagaaccgca acccgaaggc cgtgcccgat gcgtgggagc gggccgtgct gcaggatttc
     2101 gaccgccgcg ccgccgccgg cgagaacccg gcgacgctgg agaaggggga ggtcgtcgtc
     2161 gacgcaggca cgaaggtcta ccgctacatg aaggcgctgc cggtgcagcc gctgtgcctg
     2221 aattgccacg gcgcggcgga gaacatgagc ccggcgacca aggccaagct cgccgagcgc
     2281 tatccgggcg acctgggcat cggctgcgcg gtcgggcaga tccgcggcgc gatgacgatc
     2341 aagcgggcaa tgtgaccggc ggcttcacgc cggcaggaac agcgccgggt cgaccgcgac
     2401 cgtattgaga tagacggaga agtgcaggtg cggcccggtc acgcgcccgg tcgcgcctac
     2461 cttgccgatc ggcgtgcccg ccgagacgct gtcgccgacg tgcgtgtcga ttccgctcag
     2521 gtgcgcgtag agggtcagca acccctcgcc atggtcgagc acgatctgct ggcccgagaa
     2581 gaagtagtcc ccggaatcga tcacgcgccc ggcgctcgcc gcgatgaccg gcgtgccttc
     2641 cggcgccgcg atgtccatgc ccgagtgcgg attgcgcggc tggccattga agtagcggcg
     2701 cagcccgaac gaactcgagc gcgtgcccgg cgccggctgc agcagcgcca gtgattccgg
     2761 cggcgtttcg gtgaacgtcc gtagaacttc ggccatgtgc gcacgttcgc gctcatagcg
     2821 cgccagatcc tccggcgaca attccacctg cgccggcgct accttcaggt gctgggtcgc
     2881 gtacgccttg ggcccgaccg cgatctcgta ccgctgcaca ctgccttccg cgcgccgcgc
     2941 ttcgacatgc agcttcgtgc ccggcttcgt cgccagcgcg atgccgacca gcgcgatcca
     3001 ctcggcgccg tcgcgcatca cgagcactcg ctccgcaccc agacgcgcgc gcggcggaag
     3061 ctccgacgtg ccgagacgaa tgcgcgcgat gccgccgggg acggacgaca cgcgaaggga
     3121 ggttttggcg aacggcgtgg tcggcgcggc ggcgatcagc gcgggaacga gcgagagaag
     3181 aagtcggcgt cgatgcatcc gaacgaactc tattcgatgc cggcggcacc ggccgtcatt
     3241 cacgtttcag cgcggccgca gcgaggtctc ggcttgaagg agcgcccgtt ctcgccggcc
     3301 caagtccggt gcggcgattg ctcaatgcaa cgcgcaactg ccggggccat cgggatggcc
     3361 gcaactcccg caggggcccg ccattgccgg cgctgcttca ggggccgagg ccgcagtggc
     3421 gaagaccgag agtcgctttc tcaattgcgt ggaacggcac tgcgggcact gcagcacggt
     3481 gttcgaacgc acgagcgttt cgaattcgcg accgcaatcc tgacaagcgt attcaaagat
     3541 cggcatggca cggctccaac aagttttgtg attatcccgc agttcgggcc acgggactca
     3601 cggaatggaa gcctccttcc ggcgtccgct ggacagcttc cacagcagca gcgaacttcc
     3661 acccgccacg aagacataca gcgcgaagaa cgcccaggtc acgaccctcg ccgtatcgaa
     3721 cagaccgatg tgcatcagcg acgcgatcgt tgccaatgtc gcg
//
LOCUS       DBNHBY010000512         3543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_507504, whole genome shotgun sequence.
ACCESSION   DBNHBY010000512 DBNHBY010000000
VERSION     DBNHBY010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_507504"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="AC16HB_12535"
     CDS             complement(<1..823)
                     /locus_tag="AC16HB_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5607659.1"
                     /translation="MSDDFDFDTRAVRAGTIRSGFNEHSEALFLTSSFVFSSAAEAAA
                     RFSGDEPGFVYSRFSNPTVQMFEQRLASLEGAEDCLATASGMAAILAVCLATLKSGDH
                     VVCSANVFGATMQLFTNLGRFGIQTTFVRGADAGAWRAAMGPRTRLLFCETPSNPLME
                     IVDLAALAEIGRSSGALTVVDNCFCTPALQRPLTFGVDVVIHSATKYLDGQGRVLGGA
                     VLGGRGFIRENLLPVLRTTGPSLSPFNAWVLLKGLETLSLRLRAQSQTAAMLAQWL"
     gene            complement(967..2487)
                     /gene="purF"
                     /locus_tag="AC16HB_12540"
     CDS             complement(967..2487)
                     /gene="purF"
                     /locus_tag="AC16HB_12540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5607660.1"
                     /translation="MCGIVGVVATMPVNQLLYDALQLLQHRGQDAAGIATANGNAFHM
                     FKGNGLARDVFRTRNMRSLPGNSGIAQVRYPTQGSASNSEEAQPFYVNAPFGITLAHN
                     GNLTNWEQLKDEMFRIDRRHINTKSDSEVLLNVLAHELQLATERLSLDPETIFRAVEG
                     VHRRVRGSYAVVAQIAGYGLLAFRDPFGIRPLCFGVLETDQGTEYMVASESVALEGLG
                     FRFVRDVAPGEAIFVDLDGRFFSRQCAQHPTLNPCIFEYVYLARPDSVMDGVSVYGAR
                     LRLGEYLADRVAARVPTGNIDVVMPIPDSSRPAAMQLATKLGLSYREGFIKNRYIGRT
                     FIMPGQAIRAKSVRQKLNTVGVEFKGKCVLLVDDSIVRGTTIREIVQMARDAGAMRVI
                     VASAAAPVRYPNVYGIDMPTRAELIAAGRTEEEVRWEIGADALIYQRIEDLKRAVMDC
                     NPQLTNFEASCFDGHYITGDVTAAYLDRVESARTKSVVQEDGPRNQLSLAIEPADA"
     gene            complement(2503..2985)
                     /locus_tag="AC16HB_12545"
     CDS             complement(2503..2985)
                     /locus_tag="AC16HB_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR5607661.1"
                     /translation="MTFVDWAIVALVAVSLLLGVLRGFVREVISIIGWLAGAWLAIQY
                     STALGARIPLEIEWPVVKTLLAGVLIIVACVFTAAVIGWIARRLLVAARLSAVDRTMG
                     GVFGLARGVLIVAVLVYFGHGSALARQPFWRDSLVLPQFEAAVRFASRHLPVAGPTPG
                     "
     gene            complement(2987..>3543)
                     /locus_tag="AC16HB_12550"
     CDS             complement(2987..>3543)
                     /locus_tag="AC16HB_12550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5607662.1"
                     /translation="HVRTRRRLIGAAALLLAAVIVLPMVLDPTPRPVPDTVAIDIPSD
                     KTPFTPRLSSPVPAAPAGKAAAPAIEAPVKKEEAAPRPEKKAAEEPAPATAAKEAAPA
                     KPSSTGHPAFALQAAALANEAAARDLVAKLKKAGFAPYTEKVSTRDGERIRVRVGPFS
                     SREDAEQARARLKTLGIDAGVVNG"
BASE COUNT          619 a         1238 c         1124 g          562 t
ORIGIN      
        1 ccagccattg cgccagcatg gccgccgtct gcgactgcgc gcgcagccgc agtgacagcg
       61 tttcgagccc tttcagcagc acccaggcat tgaacggcga caggctcggc ccggtcgtgc
      121 gcaacacagg taacaggttt tcacggatga atccccgccc gccgaggacc gcgccgccca
      181 gaacgcgacc ctggccatcc aggtacttgg tcgccgaatg aatgacgaca tcgaccccga
      241 aagtcagcgg cctctgcagc gccggcgtgc agaaacagtt gtcgacgacc gtcagggcgc
      301 cgctgctgcg gccgatttcc gccagcgcgg cgagatcgac gatctccatc agcggattcg
      361 acggcgtctc gcagaacagc agccgcgtcc gaggacccat cgcggcgcgc cacgcgccgg
      421 cgtcggcgcc gcgaacgaag gtcgtctgga tgccgaatcg tcccaggttc gtgaagagtt
      481 gcatggtggc gccgaacacg ttggcgctgc agacgacgtg atcgccgctc ttcagtgtcg
      541 ccaggcacac ggcgaggatg gccgccatgc cggatgcggt cgccaggcag tcttccgcac
      601 cctcgagcga agcgagccgc tgctcgaaca tctgcacggt cggattgctg aagcgcgaat
      661 agacgaagcc cggctcgtcg ccggagaagc gtgccgccgc ctcggcggcg ctcgagaaca
      721 cgaagctgga ggtcaggaac agcgcttcgc tgtgctcgtt gaatccggaa cgtatcgtgc
      781 cggcgcgcac cgccctggta tcgaaatcga agtcgtcaga catgaaaaaa cccgctggca
      841 gcgcgcgcgg gtttcggtga tcgactccct cgctttagct gcatttgtta cgcgcccgca
      901 agctgagctc aaatcggcgc gacacgcaat gctacgggta ggcagccagc gcggtcaagt
      961 cgactctcag gcgtcggccg gctcgatcgc gagcgacagt tgattgcgcg gcccgtcctc
     1021 ctgcacgacg gatttcgtgc gcgccgactc gacccgatcg agataggccg ccgtcacgtc
     1081 gccggtgatg tagtggccgt cgaagcacga ggcctcgaag ttcgtcagtt gcggattgca
     1141 gtccatcact gcccgcttca ggtcctcgat gcgttgatag atcagcgcat cggcgccgat
     1201 ttcccaccgc acctcttcct ccgtgcgccc ggccgcgatc agctcggcgc gcgtcggcat
     1261 gtcgatgccg tacacgttcg ggtagcgcac cggcgccgcc gccgatgcca cgatcacccg
     1321 catcgccccg gcgtcgcgcg ccatctgcac gatctcgcgg atcgtcgtgc cgcgcacgat
     1381 cgaatcgtcc accagcagca cgcacttgcc cttgaactcg acgccgaccg tgttcagctt
     1441 ctgccgcacg gacttcgcgc ggatcgcctg acccggcatg atgaacgtgc ggccgatata
     1501 gcggttcttg atgaaaccct cccgatagct caggccgagc ttggtcgcca gctgcattgc
     1561 ggcgggtcgg ctcgaatcag gaatcggcat cacgacgtcg atgtttccgg tgggcacgcg
     1621 cgccgccacc cgatcggcca ggtattcgcc gagccgcagc cgcgcgccat agacggaaac
     1681 gccgtccatg accgaatccg gccgcgccag atacacgtac tcgaagatgc atggattcag
     1741 cgtcggatgc tgcgcgcact gccggctgaa gaaacggcca tcgagatcga cgaagatcgc
     1801 ttcgcccggc gccacgtcgc gcacgaagcg gaaccccagg ccctcgagcg caaccgactc
     1861 ggaggccacc atgtactcgg tgccctgatc ggtctcgagc acgccgaagc acagcggccg
     1921 gatgccgaac gggtcgcgga acgccagcag cccgtagccc gcgatctgcg cgacgaccgc
     1981 gtacgacccc cgcacgcgcc ggtgcacgcc ttcgaccgcg cggaagatcg tttccggatc
     2041 gagcgacagc cgctcggtag cgagctgcag ttcgtgcgcg agcacgttca gcagcacctc
     2101 ggagtccgat ttcgtattga tgtgacggcg gtcgatgcgg aacatctcgt ccttgagctg
     2161 ttcccagttg gtcagattgc cgttgtgggc aagcgtgata ccgaatggcg cgttcacgta
     2221 gaacggctgc gcctcctcgg aattcgacgc cgagccttgc gtcggatagc gaacctgcgc
     2281 gatgccggaa ttgcctggca gcgaccgcat gttgcgtgtg cggaacacat cgcgcgccag
     2341 cccattgccc ttgaacatgt ggaacgcgtt gccgtttgct gtcgcgatgc cggcggcatc
     2401 ctgtccgcga tgctgcagca gctgcagggc gtcgtagagc aactggttga ccggcatggt
     2461 ggcaaccacg ccgacaattc cgcacatcct tcgatcctcc cgctatcccg gcgtcggccc
     2521 cgcgaccggc aggtggcgcg aggcgaatcg taccgccgct tcgaactgcg gcaacacgag
     2581 cgaatcgcgc cagaacggct ggcgcgcaag cgcgctgccg tgcccgaaat acaccagcac
     2641 cgcaacgatc agcacgccgc gcgccagtcc gaacacgccc cccatggtgc ggtccaccgc
     2701 tgacagcctg gccgcaacca gcaagcggcg cgcgatccag ccgattacgg cggcagtgaa
     2761 gacgcaggcc acgatgatca gcacgccggc cagcagcgtc ttgacgaccg gccattcgat
     2821 ttccagcggg atgcgcgcgc ccagcgcggt cgaatactgg atcgccagcc aggcgccggc
     2881 gagccagccg atgatcgaga tgacctcgcg cacgaagccg cgcagcacgc cgagcagcaa
     2941 cgaaaccgcc acgagcgcga cgattgccca gtcgacgaac gtcatgtcag ccattgacga
     3001 cgccggcatc gatgccgagc gtcttcaggc gggcacgcgc ctgctcggcg tcttcgcgcg
     3061 atgaaaacgg ccccaccctg acccggatgc gctcgccgtc ccgtgtgctg actttctcgg
     3121 tgtacggcgc gaatcccgcc ttcttcagct tcgccaccag gtcgcgcgcc gcggcttcgt
     3181 tggcgagcgc cgccgcctgc agcgcgaacg ccggatgtcc cgtcgacgag ggtttcgcag
     3241 gcgccgcttc cttcgcggcg gttgcgggcg caggctcctc cgcggccttc ttctcgggtc
     3301 ttggcgccgc ttcctccttc tttacgggcg cctcgatcgc cggtgccgca gccttcccgg
     3361 caggcgcagc cggcaccggc gacgacagtc gcggcgtgaa cggcgtcttg tcgctgggaa
     3421 tgtcgatcgc caccgtatcg ggcacgggcc gcggcgtcgg atcgagcacc atcggcagca
     3481 cgatcaccgc cgccaacagg agcgcggcag cgccgatcag tcgccgacgc gtgcggacat
     3541 gca
//
LOCUS       DBNHBY010000513         2792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1725990, whole genome shotgun sequence.
ACCESSION   DBNHBY010000513 DBNHBY010000000
VERSION     DBNHBY010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1725990"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..969
                     /locus_tag="AC16HB_12555"
     CDS             13..969
                     /locus_tag="AC16HB_12555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607663.1"
                     /translation="MRFGGSLTALGLAALAALAQAQDYPKGPVRMLVPFPPGSATDLA
                     ARVMGQQLQNALGKPFVIENKAGAQGTIAGTEVVRAAPDGHTLLFTSNTAIASNVPLM
                     KKMPYDPAKDFAPVAGIGETSLVLMVRTEHPAQDLKEFIGYVKQRPGKVTAGYGSTSS
                     QISISMLNKLAGLDVLSVPYKGIPLAVNDVMGGQVDCTFVDLGNAIAQAKGGKLRALA
                     VTAPTRSPLAPDWPTLAESLPGYNITAWFVIAAPAGTPKDIVDKLHGATTRALGSAEV
                     KEKLAGVGITPMPMTPAELQKFIVAEIAKWTKLTREAGIEPE"
     gene            974..1720
                     /locus_tag="AC16HB_12560"
     CDS             974..1720
                     /locus_tag="AC16HB_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607664.1"
                     /translation="MQRVMLVTGASRGIGAAIARRAGHSGYRVGVNYHRSRDAAQAVV
                     DSVRAAGGDAVLLQADVGRLDEVQRMFGELDRAFGTLDVLVNNAGVLASFRVDESDES
                     NVPDMLRSNLLSAFFCAREAVRRMSTRHGGRGGVIINMSSVAARTGGLTGGAAYAASK
                     GALDSFTFGLAKEVGAEGIRVNAIRPGLIATDIHDVHGGLGQMEALARSAVPLGRSGS
                     AAEVAEAALWLASDASSYVHGTLIDVAGGR"
     gene            complement(1726..>2792)
                     /locus_tag="AC16HB_12565"
     CDS             complement(1726..>2792)
                     /locus_tag="AC16HB_12565"
                     /inference="COORDINATES: protein motif:HMM:NF018657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HIR5607665.1"
                     /translation="DGTANLVVARALFEPAPDAAADEADEADETAHAPAGRLFAPAFD
                     AVERSLGGVRAAVHSAVETIRHPQTALEHAARVVAGAVTLAGELAKRRDPQSPLKGEF
                     GLKKRVAWSDPVALREVKAIGAPLGAKINDVLVAGMTGALRTYLKGRGVAVDRMTLHA
                     MVPVDLRPPERAQELGNEFGLVILELAIRSRDPLARVRLTKARMDKLKRSPEPAAILA
                     LFNLFGRGPKAVEDFAVRLFGAKASLVMTNVAGPKEDLHLAGVPIDRVLFWVPHPGRE
                     LGMGISILSYKGYATLSVIADAHLVPDPEAITTQFHREFDTMLAQVKQRAAKAPASKK
                     KPARKAVTKKRRVAAKSARR"
BASE COUNT          423 a          965 c          997 g          407 t
ORIGIN      
        1 cgaggagacc gcgtgagatt cggaggatcg ctgaccgcac tcggcctcgc ggcgctcgct
       61 gcgctcgccc aggcacagga ctacccgaag gggccggtgc ggatgctggt gccgttcccg
      121 cccggcagcg ccaccgatct ggccgcgcgc gtgatggggc aacagctgca gaacgccctc
      181 ggcaagccgt tcgtgatcga gaacaaggcc ggcgcgcagg gcactatcgc cggcaccgag
      241 gtcgtgcgcg ccgcccccga cggccacacg ctgctgttca cgtcgaacac ggcgatcgca
      301 tcgaacgtgc cgctgatgaa gaaaatgccg tacgacccgg cgaaggattt cgctccggtc
      361 gccgggatcg gcgagacgtc gctggtgctg atggtgcgaa ccgagcatcc ggcgcaggac
      421 ctgaaggagt tcatcggcta cgtgaagcag cggcccggca aggtcacggc cggctacggt
      481 tcgaccagct cgcagatcag catctcgatg ctgaacaagc tcgccgggct ggacgtgctg
      541 tcggtgccgt acaagggcat tccgctcgcg gtcaacgacg tgatgggcgg gcaggtcgac
      601 tgcacgttcg tcgacctcgg caacgcgatc gcgcaggcca agggcggcaa gctgcgcgcg
      661 ctggcggtca cggcgccgac gcgcagcccg ctcgcgcccg actggccgac gctggcggag
      721 tcgctgccgg gctacaacat cacggcgtgg ttcgtgatcg cggcgccggc cggcacgccg
      781 aaggacatcg tcgacaagct gcacggcgcg accaccaggg cgctcggcag cgccgaagtg
      841 aaggagaagc tcgcgggcgt cggcatcacg ccgatgccga tgacgcctgc ggaactgcag
      901 aagttcatcg tggccgagat cgcgaaatgg acgaagctca cccgggaggc gggcatcgag
      961 cccgagtgag cccatgcaga gggtgatgct cgtcacgggc gcgagccgcg gcatcggcgc
     1021 cgcgatcgcg agacgcgccg gccattccgg ctaccgggtc ggtgtcaact atcaccgcag
     1081 ccgcgacgcg gcgcaggcgg tggtcgattc ggttcgtgcg gcgggcggcg acgccgtcct
     1141 gctgcaggcc gatgtcggcc ggctcgacga agtgcagcgg atgttcggcg aactcgaccg
     1201 tgcattcggc acgctcgacg tgctggtgaa caacgcgggc gtgctggcga gctttcgcgt
     1261 cgatgaaagc gacgagagca acgtgccgga catgctgcgc tcgaacctgc tgtccgcgtt
     1321 cttctgcgcg cgcgaggcgg tgcgccggat gtcgacgcgg cacggcggcc ggggcggcgt
     1381 gatcatcaac atgtcctcgg tcgcggcgcg caccggcggg ctcaccggcg gagcggccta
     1441 tgcggcatcg aagggcgcgc tggacagctt cacgttcgga ctggcgaagg aggtcggcgc
     1501 cgaaggcatc cgcgtcaacg cgatccgacc cgggctgatc gccaccgaca tccacgacgt
     1561 ccatggcggg ctcggacaga tggaagcgct ggcgcggtcc gccgtgccgc tcgggcgctc
     1621 gggatcggcc gccgaagtcg cggaggcggc gctgtggctc gcctccgacg cgtcgtcgta
     1681 cgtgcatggc acgctgatcg acgtcgccgg cggccgctga tccggtcagc ggcgggcaga
     1741 cttcgccgcg acgcggcgct tcttcgttac tgccttcctg gccggtttct tcttcgaggc
     1801 aggcgccttc gccgcgcgct gcttcacctg cgccagcatc gtgtcgaact cgcggtggaa
     1861 ctgcgtggtg atcgcctcgg ggtcgggcac gaggtgcgca tcggcgatca ccgacaacgt
     1921 cgcgtatccc ttgtagctca ggatgctgat gcccatgccg agttcgcggc ccggatgcgg
     1981 cacccagaac agcacgcgat cgatcggtac gccggccaga tgaaggtctt ccttcgggcc
     2041 ggcgacattg gtcatcacga ggctcgcctt ggccccgaac agccgtaccg cgaagtcctc
     2101 gaccgccttg gggccacggc cgaacaggtt gaacagcgcg aggatcgcgg ccggctccgg
     2161 cgagcgcttc agcttgtcca tgcgcgcctt ggtcagccgc acgcgcgcaa gcggatcgcg
     2221 gctgcggatg gccagttcca ggatgacgag gccgaactcg ttgccgagtt cctgcgcgcg
     2281 ctcgggcggc cgcaggtcga ccggcaccat cgcgtgcagc gtcatgcggt cgaccgcgac
     2341 accgcggcct ttcaggtaag tgcgcagcgc gccggtcatg ccggccacca gcacgtcgtt
     2401 gatcttggcg cccagcggcg cgccgatcgc cttcacctcg cggagcgcga cggggtcgga
     2461 ccacgcgacg cgtttcttca gcccgaactc gcccttcagc ggcgactgcg gatcgcgccg
     2521 cttcgccagt tcgccggcca gcgtcaccgc cccggcgacc acgcgcgccg cgtgctcgag
     2581 cgccgtctgc ggatggcgga tcgtctcgac cgccgagtgc acggccgcgc gcacgccgcc
     2641 cagcgatcgc tcgaccgcat cgaaagccgg cgcgaacagc cgcccggccg gcgcatgcgc
     2701 ggtctcatcc gcttcgtcgg cctcgtccgc cgccgcgtcc ggcgcgggtt cgaacagcgc
     2761 gcgcgccacg acgaggttgg cggtgccgtc gc
//
LOCUS       DBNHBY010000514         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_981260, whole genome shotgun sequence.
ACCESSION   DBNHBY010000514 DBNHBY010000000
VERSION     DBNHBY010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_981260"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16HB_12570"
     CDS             complement(<1..287)
                     /locus_tag="AC16HB_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5607666.1"
                     /translation="MEAEAKYTYVGAAVLLLIAALVAAVVWLKHSGSARNYTYYTIYF
                     EKQALDGLQVGGDVNTRGIKVGRVEDYALVGDKVNRVRVKIRVDRRTPVRV"
     gene            complement(277..1038)
                     /locus_tag="AC16HB_12575"
     CDS             complement(277..1038)
                     /locus_tag="AC16HB_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607667.1"
                     /translation="MSSAVIEAEGVVTRFGRQTIHDGVAFTIPRGTLAAMIGGSGTGK
                     SVLLREIIGLQRPTAGHVRVLGCDMWHSTADDVAAVRRRFGMMFQDGALFSSLTVAQN
                     VAVPLIEHAPPPRVLLDPLIQLRLAQAGLPADAGAKMPSELSGGMRKRAAIARALALE
                     PEILFLDEPTSGLDPATARGFDRLVRVLVDDLGITVLLVTHDLDTLLTVVDRLIVLAE
                     GRVIADGPVREVIEIDHPWVRSYFSIRTMVGAHGS"
     gene            complement(1035..2162)
                     /locus_tag="AC16HB_12580"
     CDS             complement(1035..2162)
                     /locus_tag="AC16HB_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5607668.1"
                     /translation="MDPTADTCNCQLLAGEGGNTLKLAGAWRLATLPAAWRNLHALRP
                     APPLALDGSALHSLDAAAALALLRALAAAGIDPQSITLHAFSEPHRRVFEDVRRRLAS
                     TEPPVPRAQRRWLAELGEKVSGLGVLLHGHLNFLGETVSALGAAARKPARLRLKELTT
                     QFEQVCLNAVPVVALVTLLIGVTIAYLLGLQAEKYGANIFVVDGVGIGMTREFAPIIV
                     ATIIAGRSGAAFTAQLGAMRLTEEIDAIRTLGLSAQQVLIVPRVLALMLALPLLVFVG
                     DIMGILGAMLVAEPMLGLTPTTFLERLHEALDITHVWVGLGKAPVFALFIAVIGCRMG
                     MTVERDTRAVGLATTSTVVQSIVAVILLDAGFAVLFQELGW"
     gene            complement(2249..>3367)
                     /locus_tag="AC16HB_12585"
     CDS             complement(2249..>3367)
                     /locus_tag="AC16HB_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5607669.1"
                     /translation="AVLSGNRNFEARIHPNLKANFLASPPLVVAYAIAGTMLRDLMTE
                     PLGKGKDGKDVWIGDVWPTSEEVYAMLKYAMNPKAFQANYGKVKKDPGELWKKIEGAA
                     GEVYVWPKSTYIAEPPFFQGFSMVPGAMPKVANARALGIFGDSITTDHISPAGSIKDT
                     SPAGKWLIENGVIKADFNSYGARRGNHEVMMRGTFANVRIKNLMLPPKADGSRVEGGV
                     TIHQPSGEQMSIYDAAMKYSASGVPTVVFGGEEYGTGSSRDWAAKGTQLLGVKAVIAR
                     SFERIHRSNLVGMGVLPLQFKGADSAASLGITGEELFDVVIDGDIRPQMDVKLVVRRK
                     DGSTQNVPLKLRIDTPIEVDYYKHGGILPFVLRQLLAA"
BASE COUNT          540 a         1162 c         1136 g          529 t
ORIGIN      
        1 ttcacgcgca ccggcgtcct gcggtcgacg cggatcttca cgcgcacgcg gttgaccttg
       61 tcgccgacca gcgcgtaatc ctcgacgcgg ccgaccttga tgccgcgcgt gttgacgtcg
      121 ccgcccacct gcaggccgtc gagcgcctgc ttttcgaagt agatcgtgta gtaggtgtag
      181 ttgcgcgctg acccggagtg cttgagccag acgacggcgg ccaccagcgc agcgatgagc
      241 agcagcaccg cggcgccaac gtaggtgtac ttggcctcag cttccatgcg cgccgaccat
      301 tgtacggatc gagaagtagg accgaaccca ggggtgatcg atctcgatca cctcgcgcac
      361 cgggccgtcg gcgatgacgc gcccctcggc cagcacgatc agccggtcga ccaccgtgag
      421 cagcgtgtcg aggtcgtgcg tgaccaacag cacggtaatg ccgaggtcgt cgaccagcac
      481 gcggacgagc cggtcgaagc cgcgtgcggt ggccgggtcg aggcccgacg tcggctcgtc
      541 gaggaaaagg atctcgggtt cgagcgcgag cgcgcgggcg atcgccgcgc gcttgcgcat
      601 gccgccggac aactcgctcg gcatcttggc gccggcgtcg gcaggcagcc ccgcctgcgc
      661 gagccgcaac tggatcaggg ggtcgagcag tacgcgcggc ggcggcgcgt gctcgatcag
      721 cggcaccgcg acattctgtg ccaccgtcag cgaggagaac agcgcgccgt cctggaacat
      781 catgccgaag cggcggcgca cggcggcgac gtcgtcggcg gtcgagtgcc acatgtcgca
      841 gccgagcacg cgcacatggc cggcggtagg ccgctgcagt ccgatgatct cgcgcagcag
      901 aaccgacttg ccggtgccgc tgccgccgat catcgccgcg agcgtgccgc gcgggatcgt
      961 gaaagcgacg ccgtcgtgga tcgtctgccg gccgaaccgc gtgacgactc cttcggcttc
     1021 gatcaccgca ctgctcacca gccaagctcc tggaacagca cggcgaagcc cgcgtcgagc
     1081 aggatcaccg caacgatgct ctgcacgacc gtcgaggtcg tcgccagtcc gaccgcgcgc
     1141 gtgtcgcgct cgacggtcat gcccatgcgg cagccgatca ccgcgatgaa cagcgcgaag
     1201 accggcgcct tgcccaggcc gacccagacg tgcgtgatgt cgagcgcctc gtgcaggcgc
     1261 tcgaggaagg tggtcggcgt cagtcccagc atcggctcgg caaccagcat cgcgccgaga
     1321 atgcccatga tgtcgccgac gaacaccagc agcggcagcg caagcatcag cgccagcacg
     1381 cgcgggacga tcagcacctg ctgcgccgac aggccaagcg tgcggatcgc gtcgatctcc
     1441 tcggtcagcc gcatcgcgcc gagttgcgcg gtgaacgccg cgccggagcg gccggcgatg
     1501 atcgtggcga cgatgatcgg cgcgaactcg cgcgtcatgc cgatgccgac gccgtcgacg
     1561 acgaaaatgt tggcgccgta cttctcggcc tgcagcccga gcaggtaggc gatcgtcacg
     1621 ccgatcagca gcgtcaccag tgccacgacg ggcacggcgt tcaggcacac ctgctcgaac
     1681 tgcgtggtga gttccttcag gcgcagccgc gccggcttgc gtgcggccgc gccgagtgcg
     1741 ctgaccgttt caccgaggaa gttcaggtga ccatgcagca gcacgcccag gccgctgacc
     1801 ttctccccca gctccgccag ccaccggcgc tgcgcgcgtg gcacgggcgg ctcggtggat
     1861 gcgagccgac gccgcacgtc ctcgaacacg cgtcgatgcg gctcgctgaa cgcatgcagc
     1921 gtgatcgatt gcgggtcgat tcccgctgcg gccaatgcac ggagcagcgc gagcgcggcc
     1981 gcggcgtcga gcgagtgcag tgcggagccg tcgagcgcca acggcggcgc cgggcgcagc
     2041 gcgtgcaaat tgcgccatgc ggccggcagc gtcgccagtc gccacgcgcc cgcgagtttc
     2101 agtgtgttgc cgccttcgcc tgccagcaac tggcagttgc acgtgtctgc ggtcgggtcc
     2161 attggcgtcg ccgcaggcag actagcgcat cgatcccgga aatgaaaacg ccggcagcaa
     2221 gccggcgttc tcgcgaccgc ggcgggcgtc aggccgccag aagctgccgc agcacgaagg
     2281 gcaggatgcc gccgtgcttg tagtagtcga cctcgatcgg cgtgtcgatg cgcagcttca
     2341 gcggcacgtt ctgcgtcgag ccgtccttgc gtcggaccac cagcttgacg tccatctgcg
     2401 gccggatgtc accgtcgatc acgacgtcga acagctcctc gcccgtgatg ccgagcgagg
     2461 ccgcgctgtc ggcgcccttg aactgcagcg gcagcacgcc cattccgacc aggttgctgc
     2521 ggtggatgcg ctcgaagctg cgcgcgatca ccgccttcac cccgagcagc tgcgtgccct
     2581 tggcggccca gtcgcgcgac gagccggtgc cgtactcctc gccgccgaag acgacggtcg
     2641 gcacgccgga ggccgagtac ttcatcgccg cgtcgtagat cgacatctgc tcgccgctcg
     2701 gctggtggat cgtgacaccg ccctcgacgc gcgagccgtc cgccttgggc ggaagcatca
     2761 ggttcttgat gcgcacgttg gcgaacgtgc cgcgcatcat cacttcgtga ttgccgcgcc
     2821 gcgcgccgta cgagttgaag tcggctttga tcacgccgtt ttcgatcagc catttgccgg
     2881 cgggcgacgt gtccttgatc gatccggccg gcgagatgtg gtcggtcgtg atcgagtcgc
     2941 cgaagatgcc aagagcgcgc gcgttggcga ccttcggcat cgcgccgggc accatcgaga
     3001 agccctggaa gaacggcggc tcggcgatgt aggtcgactt cggccagacg tagacctcgc
     3061 cggccgcgcc ctcgatcttc ttccacagtt cgcccggatc cttcttcacc ttgccgtagt
     3121 tggcctggaa ggccttcgga ttcatcgcgt atttcagcat cgcgtagacc tcttccgagg
     3181 tcggccacac atcgccgatc cacacgtcct tgccgtcctt gcccttgccg agcggctcgg
     3241 tcatcaggtc cctcagcatc gtgccggcga tcgcgtaggc gaccaccagc ggcggcgagg
     3301 cgaggaagtt cgccttgagg ttcggatgga tgcgcgcctc gaagttgcgg ttgccggaca
     3361 gcacggc
//
LOCUS       DBNHBY010000515         3554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1320215, whole genome shotgun sequence.
ACCESSION   DBNHBY010000515 DBNHBY010000000
VERSION     DBNHBY010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1320215"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16HB_12590"
     CDS             <1..374
                     /locus_tag="AC16HB_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607670.1"
                     /translation="AARVATASPEQVARRTAVVNELQNRECVVSLESINRLARSGTPV
                     DPQEFERLQQTADRTCSKDPVTRRQQEEAAARLRVARGDTCVDARNKLADMLEPGQRP
                     TREQLKNQQEFIESHCTPPVR"
     gene            complement(397..1020)
                     /locus_tag="AC16HB_12595"
     CDS             complement(397..1020)
                     /locus_tag="AC16HB_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5607671.1"
                     /translation="MPGKVPRGSFRRRDRGVFSPLGRLILDKLDRGVMLLDAEGSVID
                     ANTIAEKVLVLQHGMLVRNGRLAFADAELDAKFARLLNHHSRSAARSLAVRVKPRGAG
                     SFRALVSTVGPQALAREAAYLVVLYAPGEQRLISREVLLELYGLTRAQADVARLLYAG
                     HSVEETASKLQLSLNTVRTHLKHIFSKCEVQSQAELMHTFALGPQSL"
     gene            complement(1316..1741)
                     /locus_tag="AC16HB_12600"
     CDS             complement(1316..1741)
                     /locus_tag="AC16HB_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607672.1"
                     /translation="MRTLLALWAVWGTAASAQPPPQLVIAHRLVAEFVGTEQAQGTIV
                     LTLTNRSDEPLRGVRLSLADSSDGQVTGAVNEALELAPHETRTVDGRFQFDPAALAPD
                     HAIEWYVLYRDADGYSLQARVASTGGTASALARADSSTP"
     gene            complement(2085..2339)
                     /locus_tag="AC16HB_12605"
     CDS             complement(2085..2339)
                     /locus_tag="AC16HB_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607673.1"
                     /translation="MKLVLLIPLLLVLAAVLFVNSRRREARQREAEAAARAKAAKRNR
                     VPAVSNNLKGVTATQTLQPVRPTNPSGTRPTSREDDDRAA"
     gene            complement(2520..>3554)
                     /locus_tag="AC16HB_12610"
     CDS             complement(2520..>3554)
                     /locus_tag="AC16HB_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5607674.1"
                     /translation="FINYPTRFDSSETQALLDKANIRVPKLEDYAWRLWDYWERHLDP
                     DLSIDRSLRGAVKGKVVVITGGSSGIGEAAAVRVADAGGKVVIVARDEEKLAEVRKRI
                     TKSGGFVKTYSCDITDYAANDKLARDILKEFGQVDVLVNNAGRSIRRSLALSYDRFHD
                     FERTIQLNYFACVRLTMNLLPSMTARKSGHVINVSSIGVLTNAPRFSAYVASKAALES
                     FARCAASEFHDENVHFTIINMPLVRTPMIAPTKLYDEMPTIISPDEAADMIADAIIRK
                     PQRIATRLGVMAEIMHLVMPKLSEVVMNSAYRMFPDSAAAQGRREGEEQRPPTREAMI
                     FASLMRGIHW"
BASE COUNT          580 a         1189 c         1215 g          570 t
ORIGIN      
        1 gcgccgcgcg cgtcgcgacc gcctcgcccg aacaggtggc acggcggacg gccgtcgtga
       61 acgaactgca gaaccgcgaa tgcgtggttt cgctcgaatc catcaaccgc ctcgcgcgca
      121 gcggcacacc ggtcgacccg caggaattcg agcgcctgca gcagacggcg gaccgcacct
      181 gcagcaagga cccggtgacg cgccgccagc aggaagaggc cgcggcacga ttgcgcgtgg
      241 cgcgcggcga cacgtgcgtc gatgcacgca acaagcttgc tgacatgctc gaacccggcc
      301 agcgcccgac gcgcgagcag ctgaagaacc agcaggaatt catcgagtcg cactgcaccc
      361 cgcccgtgcg ctgagcgagg ccggcgggaa cgcgcgtcag aggctctgcg gcccgagcgc
      421 gaacgtgtgc atcagctctg cctgcgactg cacttcgcac ttcgagaaga tgtgcttcag
      481 gtgggtgcgc accgtgttca gcgacagctg cagtttcgac gcggtctctt cgacggagtg
      541 cccggcgtag agcagccgcg cgacgtcggc ctgggcacgg gtgagaccgt agagttcgag
      601 cagcacttcg cgcgagatca ggcgctgctc ccccggtgcg tacagcacca cgagatacgc
      661 ggcttcgcgc gccagcgcct gcggaccgac ggtcgagacc agcgcacgga acgaccccgc
      721 gccgcgcggc ttaacccgca ccgcgagcga gcgcgctgcg gagcgggaat gatggttgag
      781 caggcgcgcg aacttcgcgt ccaactcagc gtcggcaaag gcaagacgac cgttgcgcac
      841 cagcatgccg tgctgcagca cgagcacctt ctcggcgatc gtgttcgcgt cgatgacgga
      901 gccttccgca tcgagcagca tcacgccccg gtcgagcttg tcgaggatca ggcgtccgag
      961 cggagagaag acgccgcggt cgcgccgacg gaaggagccg cgcggcacct tccctggcac
     1021 cgcgcgaccc ccattggcct gtgccggagc tgcggtggcc cggcttgcca cttgcgttcc
     1081 ggcgccatca ccggacacgc gggtctttcg ggccttggtc ccaaggccgc gtcgattcga
     1141 catcatcccg tcgctttgtg cagcctgcgg ctgtcgtcct tgtggtccta ctagaccaca
     1201 gacgcgcgac aaatgaatca cccaaatagg tgatgctgcg gtaaacacgg tgagcagcgc
     1261 gatggggcgc aggctccacg cgcgatgcgg gaaacaagaa tcggtcgcgg cgtgcctacg
     1321 gagtcgatga gtcggcccgg gcgagcgcgg atgcggtccc gcccgtgctg gcaacacggg
     1381 cctgcaggct gtagccgtcg gcatcgcgat acagcacgta ccactcgatc gcgtggtcgg
     1441 gcgcaagggc cgctggatcg aactggaagc ggccgtcgac cgtgcgggtc tcgtgggggg
     1501 caagttcgag cgcttcgttg accgctccgg tcacctgtcc gtcagacgag tcggccaggc
     1561 tcagcctcac accgcgcaag ggttcgtcgg agcgattggt cagcgtcagg acaatcgtcc
     1621 cctgtgcctg ttccgtgccg acgaactccg cgaccaggcg atgggcgatg accagctgcg
     1681 gcgggggttg cgccgaggcc gccgttcccc acactgccca cagagccagc agcgttcgca
     1741 tcgctgcctg ccatccacgc tggtttcgct gcaaggcggg acctcctgtc ggtcggccat
     1801 tcgcgatgac ggccacgtgc ggcccgtcat cgaagcgcgc catcctatgg ggcctgtcgt
     1861 cgccgcggca tcagacagac gccgacacgg accgtgatca tcgtgggatg tgcgtacgcg
     1921 atttcgaacg ccgtcccgtt cacggaatgc ccgccaggcc cggcgcatag tgcgtccgtt
     1981 gcgcggcgca tcgccgcagc ggagacaagc atggaagtcg cggcgttcgt cctgctcggt
     2041 tcgatcgcgc tctggttcgc cgaggaactg cacacacagc ggcgctaagc cgcgcggtcg
     2101 tcgtcttccc tgctcgtcgg gcgcgtgccc gacgggttag tcggccggac cggctgcagc
     2161 gtctgcgttg cggtgacgcc tttcaggttg ttcgagaccg ccggcacacg gttccgcttg
     2221 gcagccttgg ctcgggccgc cgcctcggcc tcgcgctggc gcgcctcacg ccgccgtgaa
     2281 ttgacgaaca gcaccgcggc cagcaccagc agcagcggga tcagcagtac gagcttcaaa
     2341 cggacctcct tccatggcgg ggcggcggca ggccggtccc gacgagcggt cttgccaggc
     2401 gccacggacg aggtggcgcc ggttccttgc aatctagcgc ggcaggcgcc gcagcagaag
     2461 cggcgattct acccgcttgc cgtcgattcg tcgtgacggc ggcccgcttc gtcgaatcgc
     2521 taccagtgga tgccacgcat cagcgaggcg aagatcatgg cctcgcgtgt aggaggacgc
     2581 tgctcctcgc cttcgcgacg gccctgggcc gccgcggagt ccgggaacat gcggtacgcg
     2641 gagttcatga ccacctcgga cagcttcggc atgacgaggt gcatgatctc cgccatcacg
     2701 ccgagccggg tcgcgatgcg ctgcggcttg cggatgatgg catcggcgat catgtccgcc
     2761 gcctcgtccg gcgagatgat cgtcggcatc tcgtcgtaca gcttcgtggg cgcgatcatc
     2821 ggcgtgcgca ccagcggcat gttgatgatc gtgaagtgca cgttctcgtc gtggaactcg
     2881 gacgccgcgc agcgcgcgaa cgattccagt gcggccttgg acgcgacata ggccgagaac
     2941 cgcggggcat tggtcagcac gccgatcgag gacacgttga tcacgtggcc cgacttgcgc
     3001 gcggtcatcg acggcagcag gttcatcgtc agccgcacgc acgcgaagta gttcagctgg
     3061 atggtgcgct cgaagtcgtg gaagcggtcg tacgacagcg cgagcgagcg acggatggag
     3121 cgtcccgcgt tgttgaccag gacgtcgacc tgcccgaact ccttgaggat gtcgcgcgcg
     3181 agcttgtcgt tcgcggcgta gtcggtgatg tcacaggagt acgtcttgac gaagccgccg
     3241 gacttggtga tgcgcttgcg cacctcggcc agcttctcct cgtcgcgcgc gacgatcacg
     3301 accttgccgc cggcatccgc gacgcgcacg gcggccgctt cgccgatgcc cgaagagccg
     3361 cccgtgatga cgaccacctt gcccttgacc gcaccgcgca ggctgcggtc gatggagagg
     3421 tccgggtcga ggtggcgctc ccagtagtcc cacagccgcc acgcgtagtc ctcgagcttg
     3481 ggcacgcgga tgttggcctt gtcgagcagg gcctgcgtct cgctcgagtc gaagcgcgtc
     3541 gggtagttga tgaa
//
LOCUS       DBNHBY010000516         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1659142, whole genome shotgun sequence.
ACCESSION   DBNHBY010000516 DBNHBY010000000
VERSION     DBNHBY010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1659142"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="AC16HB_12615"
     CDS             <1..901
                     /locus_tag="AC16HB_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607675.1"
                     /translation="EAQDEDRAIALARGALAAGRGIAAIERTLAQAALAHYADFGHSL
                     IYLVHARTLVERLGRSIELPLLLALVRSYVRATREDLLPDFRGYPDALAAWPASPGRA
                     TATAAPLQTDTVKRALQQTLADAARLGTAALYWRLLHANALNLRRFDTRFEQLAPDKP
                     GEDVGWLDVTHGITFANAVRVQCERFPEFWPQGLLQMALFAGRNARYAARDTQEPEAG
                     ADAFDGGALDCVLDHGIGLYIHSAHKLKTWLAAREEIAAGTPAAVADALRAGVARYLQ
                     ANVKQKHTRRAARLALAFVARED"
     gene            951..1349
                     /locus_tag="AC16HB_12620"
     CDS             951..1349
                     /locus_tag="AC16HB_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR5607676.1"
                     /translation="MSGMDWPALVTLGALTLGLATAWLVGHARVKYGIKAPATTGHEM
                     FERAYRVQMNTLENTVLFLPALWLAAHYVGALAATVLGGVWLAGRVWYAFAYMANPKK
                     RGPGFTLAYVAWGLMMVGAAWGVLGNLAGK"
     gene            1349..>1747
                     /locus_tag="AC16HB_12625"
     CDS             1349..>1747
                     /locus_tag="AC16HB_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5607677.1"
                     /translation="MAAQATASAATRTTATPAPAARRLRPLRSLLPYVTVYRKQIALA
                     LLFLLLAAASTLALPYAVKLLVDGGLALPAGTEVGARLVAIREHFLGLFGVSVALGLF
                     TAGRCYMVTWIGERVTTDLRQAVYAHVLQQS"
BASE COUNT          240 a          621 c          623 g          263 t
ORIGIN      
        1 cgaggcgcag gacgaggacc gcgcgatcgc gctcgcgcgc ggtgcgctgg ccgcaggacg
       61 aggcatcgcc gcgatcgaac gcacgctggc gcaggcggcg ctggcgcact atgcggactt
      121 cggccacagc ctgatctatc tggtgcacgc gcgcacgctg gtcgagcggc tcggccgatc
      181 gatcgagttg ccgctgctgc tcgcgctggt gcgctcctac gtgcgcgcca cgcgcgagga
      241 cctgctgccc gatttccgcg gctatcccga tgcgctcgcc gcatggccgg cctcacccgg
      301 ccgcgccacc gcgacggcgg caccgctgca gacggacacg gtcaagcggg ccctgcagca
      361 gacgctggcc gacgcggcgc ggctcggaac cgcggcgctg tattggcgtc tgctgcacgc
      421 gaacgcgctg aacctgcgtc ggttcgacac ccgcttcgag cagctcgcgc ccgacaagcc
      481 gggcgaggac gtcggctggc tcgacgtgac gcacggcatc accttcgcca atgccgtgcg
      541 cgtgcagtgc gaacgctttc ccgagttctg gccgcaggga ctgctgcaga tggcgctgtt
      601 tgccggccgc aatgcgcgct acgccgcacg cgacacgcag gaaccggagg ccggcgccga
      661 cgcgttcgac ggcggcgcgc tcgactgcgt gctcgaccac ggcatcgggc tttacattca
      721 ttcggcgcac aagctgaaga cctggctcgc tgcccgcgaa gaaatcgccg cgggcacacc
      781 cgccgccgtc gccgacgcgc tgcgcgcggg cgtggcgcgt tacctgcagg cgaacgtgaa
      841 gcagaagcac acgcggcggg cggcgcgact ggcgctcgca ttcgttgcgc gcgaggactg
      901 acgcgcgacc ctcgcgtagc atttcgattt cccgtttgct cgaggattgc atgagcggca
      961 tggactggcc cgcgctcgtc acgctgggcg cgctgacgct gggcctcgcg accgcatggc
     1021 tggtcggcca cgcgcgcgtc aagtacggca tcaaggcccc cgcgacgacc ggccacgaga
     1081 tgttcgagcg cgcgtaccgc gtgcagatga acacgctcga aaacaccgtg ctgttcctgc
     1141 cggcgctgtg gctggcggcg cactacgtcg gcgccctggc ggcgaccgtg ctcggcggcg
     1201 tgtggctggc cggccgcgtc tggtacgcat tcgcatacat ggccaacccg aagaagcgcg
     1261 gcccgggctt cacgctcgcc tacgtcgcgt gggggctgat gatggtcggc gccgcgtggg
     1321 gcgtgctcgg caatctcgcg ggcaagtgat ggcagcgcag gcaacggcat ccgccgcgac
     1381 ccggacgacc gcaacgccgg caccggcggc gcgccggttg cgtccgctgc gctccctgct
     1441 gccgtacgtg acggtctacc gcaagcagat cgcgcttgca ctgctcttcc tgctgctggc
     1501 ggcggcgagc acgctggcgc tgccgtatgc agtcaagctg ctggtcgacg gcggactcgc
     1561 gctgccggcc ggcaccgagg tcggcgcgcg gctcgtggcg attcgcgagc acttcctggg
     1621 cctgttcggc gtgtcggtcg cgctcggcct tttcaccgcg ggacgctgct acatggtcac
     1681 gtggatcggc gagcgcgtga cgaccgacct ccgtcaggcc gtctacgcgc acgtgctgca
     1741 gcagagc
//
LOCUS       DBNHBY010000517         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_101371, whole genome shotgun sequence.
ACCESSION   DBNHBY010000517 DBNHBY010000000
VERSION     DBNHBY010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_101371"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16HB_12630"
     CDS             <1..210
                     /locus_tag="AC16HB_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607678.1"
                     /translation="GRVTFYEEMATVKYMRTQPDRCFPIKTVPAVAVAGYYPTQSCIR
                     YAKDKAEYTVSLESFQLRAASPPGK"
     gene            complement(215..703)
                     /locus_tag="AC16HB_12635"
     CDS             complement(215..703)
                     /locus_tag="AC16HB_12635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5607679.1"
                     /translation="MIKGLHHNAYRCRDSEETRRFYEDFLGLPLTGSLEIRETKTGRR
                     TSVLHTFYRLDDGSFLAFFEAPDMPFEFKQQHDYDLHIALEVEPDVLDAMFARGKERG
                     IETRGISEHGFVRSIYFRDPNGYVIELTAKTPQHDSMMDPAANEARAILDRWQVEKAR
                     AG"
     gene            complement(718..1392)
                     /locus_tag="AC16HB_12640"
     CDS             complement(718..1392)
                     /locus_tag="AC16HB_12640"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5607680.1"
                     /translation="MKLYCHPVSTTSRPILLFAAETGIELDFQIVDLFTGEQYKPEYA
                     AINPSSQVPVLDDGDFRLTESSAILKYLAEKTGSPAYPADPRKRARVNERMDWFNTGF
                     YRDFAYNFVYPQIFPFMRRPDEKVQAGTIAWGRDKALFWLKVLDGSLIGSNRFVCGNE
                     ISLADYLGAMMVLGGEAIGQSFSAYPNISRWLGTMKALKNWAKVNEAFYKYVVEPGKG
                     KEFVRL"
     gene            1613..2602
                     /gene="dctP"
                     /locus_tag="AC16HB_12645"
     CDS             1613..2602
                     /gene="dctP"
                     /locus_tag="AC16HB_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR5607681.1"
                     /translation="MTISRRAVLAAASIAVAAVSGRVDAQEVTINAVHFTPAQVSYAK
                     SFLQFVDKVNQRGKGVVQIKVRGGPEIMPLAQLGEAQKNGLIDMIDCPAGPYLNLVPE
                     GEVFAATTKKPWELRANGGFALINEIFAKKGNAYVLAQVDGGAGFHIFTTEEPKRGAD
                     GSIDFTKLKIRSAALYRDFLESLGAGVVVQGPGEAYTSLERGLVNANAYTVLGYQGFG
                     WQKFTKYRIDPGFFQTDVLISMNKAKWDSLPEAARRILTEVALEHERESYEANLKGTE
                     EEGRQMIAGGMKVVELTGAARAAFLDKATRASWDRMNKRDPTHIAALREKFGN"
     gene            2613..3164
                     /locus_tag="AC16HB_12650"
     CDS             2613..3164
                     /locus_tag="AC16HB_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5607682.1"
                     /translation="MPSPEQGATSRRGALAPLFAASGAIAHALAQITKVLLALIVLLV
                     AADVAARNLAKPLVWSVSLTEYLLLYITFLPMPALVRGKGHVCADFIRSALPARLKRA
                     IEIGVYALCLAICAYLGAVALHGALDSFRSGAYDVRTFDMPRWLIYAPMAVGLWLSAL
                     EFLRYLLGHDSIYALDAREMEGF"
     gene            3169..>4155
                     /locus_tag="AC16HB_12655"
     CDS             3169..>4155
                     /locus_tag="AC16HB_12655"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5607683.1"
                     /translation="MDWLVALTLMVGLILALMGMGVPVAFAFFATNIVCLYLFMGGSV
                     GVAQMVTNFGDAVSIHALTPMPLFLIMGSLFFRSGLGDGVFKAIDLCIGNLRARLSYL
                     VVLAGAVFAALSGSSLANTGMMGSAMVPEMLRRGYKPHMSFGPVLGAGGLAVIIPPSS
                     LAVLLGSLAQIDVGALLVAGFLPGLLLTALYLTLITVQAAIDVDAAPQYDAPRASLRE
                     KFAAIVANVVPMGVIVFLVVGTIILGIATPTESAALGCLGVMGLLLAYGRFSWRVVWK
                     SLDDAMKVTGMTFLIIAASTTFAQVLAFSGASNGMIQWLISFNWTPYAMLAVM"
BASE COUNT          666 a         1359 c         1394 g          736 t
ORIGIN      
        1 ggccgggtga cgttctacga ggagatggcc accgtcaagt acatgagaac gcaaccggat
       61 cgctgcttcc cgatcaagac ggtgccggcg gtcgccgtcg cgggttacta cccgacgcag
      121 tcgtgcatcc gctatgcgaa ggacaaggcc gagtacacgg tgtcgctcga gtcgttccag
      181 ctgcgcgcgg cgagtccgcc gggcaagtaa cgcctcagcc ggcgcgcgcc ttttcgacct
      241 gccagcgatc gaggatcgcg cgcgcctcgt tggccgccgg atccatcatc gaatcgtgct
      301 gcggcgtctt cgcggtcagt tcgatcacgt agccgttcgg gtcgcggaag tagatggagc
      361 gcacgaatcc gtgctccgag atgccgcgcg tctcgatgcc gcgctccttg ccgcgggcaa
      421 acatcgcgtc cagcacatcc ggctcgacct cgagcgcgat gtgcaggtcg tagtcgtgct
      481 gctgcttgaa ctcgaacggc atgtccggcg cctcgaagaa cgcgaggaac gaaccgtcgt
      541 cgagccggta gaacgtatgc agaaccgaag tcctgcgacc ggtcttggtc tcgcggatct
      601 cgaggctgcc ggtgagcggc agcccgagaa agtcctcgta gaagcgccgc gtctcctcgg
      661 agtcgcggca gcggtaggcg ttgtggtgca gtcccttgat catcgaccgc ctccgattca
      721 aagccgaacg aactccttgc ccttgcccgg ctcgacgacg tacttgtaga acgcctcgtt
      781 gaccttcgcc cagttcttaa gggctttcat cgtgcccagc cagcgcgaga tgttcggata
      841 cgcgctgaag gactgtccga tcgcctcgcc gccgagcacc atcatcgcgc cgaggtagtc
      901 ggcgagcgag atctcgttgc cgcagacgaa gcggttggaa ccgatcaggc tgccgtcgag
      961 caccttcagc cagaacaggg ccttgtcccg gccccaggca atcgtccccg cctgcacctt
     1021 ctcgtccggc cggcgcatga agggaaagat ctgcgggtaa acgaagttgt aggcgaagtc
     1081 ccggtagaag ccggtattga accagtccat ccgctcgttg acacgcgccc gcttgcgtgg
     1141 atccgccgga tacgcgggcg agcccgtctt ctccgccagg tatttcagga tcgcggaact
     1201 ctccgtcagg cggaagtcgc cgtcatcgag caccggcacc tggctgctcg gattgatcgc
     1261 cgcgtactct ggcttgtact gctcgccggt gaagagatcg acgatctgga agtcgagttc
     1321 gatgccggtc tcggccgcga acagcaggat cggccggctc gtcgtcgaca ccggatggca
     1381 atagagtttc atagttgtcc cctcgtcccg tcaccggtgg ccgcacgcgg ccgcccggtg
     1441 cgaggcgcag aggctactcc cgcgccagcg agccgacaat gcaggtcatc aactgcgcga
     1501 ccgcgccgat cttcttgttg ccccatcgca cgacgcttac acttttccgc cgcggtcgcc
     1561 gacaagagcg tcgcggcccg ttcgggccca acatcgacaa gagagacaac ccatgacgat
     1621 ttctcgccgc gctgtccttg ccgccgcctc gattgccgtc gccgcagttt ctggccgcgt
     1681 cgacgcgcag gaagtcacga tcaacgcggt gcacttcacg ccggcgcagg tcagctacgc
     1741 gaagagcttc ctgcagttcg tcgacaaggt gaaccagcgc ggcaagggcg tggtgcagat
     1801 caaggtgcgc ggcggccccg agatcatgcc gctggcgcag ctcggcgagg cgcagaagaa
     1861 cgggctgatc gacatgatcg actgcccggc cgggccgtac ctgaacctgg tgcccgaggg
     1921 cgaggtgttc gcggcgacga ccaagaagcc gtgggagctg cgcgccaacg gcggcttcgc
     1981 gctgatcaac gagatcttcg cgaagaaggg caacgcctac gtgctggccc aagtcgacgg
     2041 cggcgccggt ttccacatct tcacgaccga ggagccgaag cgcggcgccg acggcagcat
     2101 cgacttcacg aagctgaaga tccgcagcgc cgcgctgtac cgcgacttcc tcgaaagcct
     2161 cggcgccggc gtcgtcgtgc agggcccggg cgaggcgtac acgtcgctgg agcgcgggct
     2221 ggtgaacgcc aatgcgtaca cggtgctcgg ctaccagggc ttcggctggc agaagttcac
     2281 caagtaccgc atcgatccgg gcttcttcca gaccgacgtg ctgatcagca tgaacaaggc
     2341 caagtgggac agcttgccgg aggccgcgcg ccgcatcctg accgaggtcg cgctggaaca
     2401 cgagcgcgag agctacgaag ccaacctcaa gggcaccgag gaagagggcc ggcagatgat
     2461 cgccggcggc atgaaggtgg tcgaactcac cggtgcggcg cgcgccgcgt tcctcgacaa
     2521 ggccacgcgc gcaagctggg accggatgaa caagcgcgac cccacccaca tcgccgcgct
     2581 gcgcgagaag ttcggcaact gagcggccgg cgatgccgtc gccggaacag ggcgcgacga
     2641 gccggcgcgg cgcgctggcg ccgctgttcg ccgccagcgg cgcgatcgcg cacgcgctgg
     2701 cgcagatcac caaggtgctg ctggcgctga tcgtgctgct ggtcgcggcc gacgtggcgg
     2761 cgcgtaacct ggccaagccg ctggtgtgga gcgtgtcgct gaccgagtac ctgcttctgt
     2821 acatcacgtt cctgccgatg ccggcgctcg tgcgcggcaa gggccatgtc tgcgccgact
     2881 tcatccgcag cgcgctgccg gcgcgactga agcgcgcgat cgagatcggc gtgtacgcgc
     2941 tgtgcctggc gatctgcgcc tatctgggcg cggtcgcgct gcacggcgcg ctcgacagct
     3001 tccgtagtgg cgcctacgac gtgcgcacct tcgacatgcc gcgctggctg atctacgcgc
     3061 cgatggcggt cgggctgtgg ttgtcggcgc tggagttcct gcgctacctg ctgggacacg
     3121 attcgatcta cgccctcgac gcgcgcgaga tggaaggttt ctgaggcgat ggactggctg
     3181 gtcgcgctga cgctgatggt cggcctgatc ctcgcgctga tgggcatggg cgtgccggtg
     3241 gcgttcgctt tcttcgcgac caacatcgtg tgcctgtatc tgttcatggg cggatcggtc
     3301 ggcgtggcgc agatggtgac caacttcggc gacgcggtgt cgatccacgc gctgacgccg
     3361 atgccgctgt tcctgatcat gggcagcctg ttcttccgtt ccggtctcgg cgatggcgtg
     3421 ttcaaggcga tcgacctgtg catcggaaac ctgcgtgcgc ggctttcgta cctggtcgtg
     3481 cttgccggcg cggtgttcgc ggcgctgtcg ggctcgagcc tcgccaacac cggcatgatg
     3541 ggctcggcga tggtgccgga gatgctccgg cgcggctaca agccgcacat gagcttcggc
     3601 cccgtgctcg gcgcgggcgg gctggcggtg atcattccgc cgtcgtcgct ggcggtgctg
     3661 ctgggatctc tcgcacagat cgacgtcggc gcgctgctgg tcgccggctt cctgcccggc
     3721 ctgctgctga ccgcgctgta cctgacgctg atcacggtgc aggcggcaat cgacgtcgac
     3781 gccgcacccc agtacgacgc gccgcgcgcc agcctgcgcg agaaattcgc cgcgatcgtc
     3841 gccaacgtgg tgccgatggg cgtgatcgtg ttcctcgtcg tcggcacgat catcctcggc
     3901 atcgccacgc cgacggaatc ggccgcgctc ggttgcctgg gggtcatggg gttgctgctc
     3961 gcctacggcc gcttctcttg gcgcgtggtg tggaagtcgc tcgacgacgc gatgaaggtc
     4021 accggcatga cgttcctgat catcgccgcg tcgaccacgt tcgcgcaggt gctggcgttt
     4081 tccggcgcct ccaacggcat gatccagtgg ctgatctcgt tcaactggac accgtatgcg
     4141 atgctggcgg tgatg
//
LOCUS       DBNHBY010000518         3699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1048686, whole genome shotgun sequence.
ACCESSION   DBNHBY010000518 DBNHBY010000000
VERSION     DBNHBY010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1048686"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC16HB_12660"
     CDS             <1..727
                     /locus_tag="AC16HB_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5607684.1"
                     /translation="VRENQIARLAKVKATRDSAKVNAALAALTECARAGRGNLLDLSI
                     QAIRARATVGEVSSALETVFGRHRADIQKVTGVYAMAYQDADGAAQEWSRLKADIAAF
                     AEQHGRRPRVMVAKLGQDGHDRGSKVIATAYADLGFDVDIGPLFQTPEECARQAIEND
                     VHAIGVSTLAAGHKTLVPAIIAELKKQGADDIAVFVGGVIPKQDYEFLFAAGVKGIFG
                     PGTPIPVSAREVLAQIRKAHAGS"
     gene            741..1736
                     /gene="meaB"
                     /locus_tag="AC16HB_12665"
     CDS             741..1736
                     /gene="meaB"
                     /locus_tag="AC16HB_12665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR5607685.1"
                     /translation="MAAADQALIDQVLGHSGPAQRRALAKTITLLESQRADHRARADA
                     MLNALLPASGKSFRLGISGVPGVGKSTLIEALGLYLIERGHRIAVLAVDPSSSVSGGS
                     ILGDKTRMERLSVDARAFIRPSPAGGTLGGVAEKTREAMLACEAAGHDVVIVETVGVG
                     QSETAVAGMTDMFVLMQLPNAGDDLQAIKKGVMELADLVVINKADLDRAAATRAQAQI
                     TSALRIFGLHGNPEQGHAQPQVMQVSALNADGIDRFWTAVERFRERQNASGALAARRR
                     AQDQAWMWERIEAGLKQRFRSHPRVRTALPELTEQVQKGHIAASVAARRLLDLMD"
     gene            1762..3294
                     /locus_tag="AC16HB_12670"
     CDS             1762..3294
                     /locus_tag="AC16HB_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5607686.1"
                     /translation="MHEIIGQLEQKRAAARLGGGQRRIDAQHAKGKLTARERLELLLD
                     EGSFEEWDMFVEHRCVDFGMAEQKVPGDGVVTGYGTINGRMVFVFSQDFTVFGGALSE
                     AHAEKICKIMDHAMKAGVPVIGLNDSGGARIQEGVASLAGYADVFQRNVLASGVIPQI
                     SMIMGPCAGGAVYSPAMTDFIFMVKDSAYMFVTGPEVVRTVTHEEVTAEELGGAITHS
                     TRSGVADLAFENDVEALLMLRRLYNYLPLNNREKPPVRRTDDPADRMDASLDTLVPSN
                     PNKPYDMKELIIKTVDDRDFFEIQPDYAKNIIVGFARMDGHTVGIVANQPLVLAGCLD
                     IRSSIKAARFVRFCDSFNIPVITFVDVPGFMPGTAQEYGGIIKHGAKLLFAFAECTVP
                     KVTVITRKAYGGAYDVMSSKHLRGDVNLAWPHAEIAVMGPKGAVEIIFREDKNEPDKL
                     AAREAEYKARFANPFVAGARGFIDDVIQPHETRKRICRSLVMLRNKRLENPWRKHGNI
                     PL"
     gene            3314..>3699
                     /locus_tag="AC16HB_12675"
     CDS             3314..>3699
                     /locus_tag="AC16HB_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR5607687.1"
                     /translation="MFSKILIANRGEIACRVIKTAKKMGIRTVAVYSEADRDALHVEL
                     ADEAVPIGGAPSRDSYLVMDKIIAACKQTGAQAVHPGYGFLSENEEFARRVEDEGIVF
                     IGPKHYSIAAMGDKIASKKLALEAQV"
BASE COUNT          667 a         1173 c         1326 g          533 t
ORIGIN      
        1 ggtgcgcgag aaccagatcg cgcggctcgc gaaggtgaag gcgacgcgcg acagcgccaa
       61 ggtgaatgcg gcgctcgcgg cgctgaccga atgcgcgcgc gctggccgcg gcaacctgct
      121 cgatctgtcg atccaggcga tccgggcccg cgcgaccgtg ggcgaggtca gcagcgcgct
      181 cgaaacggtt ttcgggcggc accgcgccga catccagaaa gtcacggggg tctatgcgat
      241 ggcctatcag gacgcagacg gcgcggcgca ggagtggagc cgcctgaaag ccgacatcgc
      301 cgcctttgcc gagcagcacg gccgccggcc gcgcgtgatg gtcgccaagc tcggccagga
      361 cggacacgac cgcgggtcga aggtgatcgc gaccgcgtat gcggacctcg gcttcgacgt
      421 cgacatcggg ccgctgttcc agacgccgga ggagtgcgcg cggcaggcga tcgagaacga
      481 tgtgcacgcg atcggcgtat ccacgctcgc cgccgggcac aagacgctgg tgccggcgat
      541 catcgccgag ctgaagaagc agggcgccga tgacatcgcg gtcttcgtcg gcggcgtgat
      601 tccgaagcag gactacgagt tcctgttcgc ggcgggcgtc aagggcatct tcggccccgg
      661 caccccgatc cccgtcagcg cccgcgaggt gctggcgcag atccgcaagg cgcacgcagg
      721 cagctaaggc ggcacgcgtg ctggcagccg cagatcaggc gctgatcgat caggtcctcg
      781 ggcattccgg tcccgcgcag cggcgcgcgc tggcgaagac gatcacgctg ctggaatcgc
      841 agcgcgccga tcatcgcgcg cgcgcggatg cgatgctcaa tgcgctgctg cctgcgagcg
      901 gcaaatcgtt ccggctcggc atctccgggg tgccgggcgt cggaaagtcg acgctgatcg
      961 aggcgctcgg cctctatctg atcgaacgcg ggcaccgaat cgcggtgctg gcggtcgatc
     1021 cctcgtcgag cgtgtcgggc ggctcgatcc tcggcgacaa gacgcggatg gagcggctgt
     1081 cggtcgacgc ccgcgcattc atccgcccga gccccgccgg cggcacgctg ggcggcgtcg
     1141 cggagaaaac gcgcgaggcg atgctggcgt gcgaggcggc gggccacgac gtcgtcatcg
     1201 tcgagaccgt cggcgtgggc cagagcgaaa ccgcggtcgc cggcatgacc gacatgttcg
     1261 tgctgatgca gctgccgaac gccggcgacg atctgcaggc gatcaagaag ggcgtgatgg
     1321 agctggcgga cctggtcgtc atcaacaagg cggacctcga ccgggccgct gccacgcgcg
     1381 cgcaggcgca gatcacgtcc gcgctgcgga tcttcgggct gcacggcaat ccggagcagg
     1441 gccacgcgca gccgcaagtg atgcaggtga gcgcgctgaa tgccgacggc atcgaccgct
     1501 tctggactgc cgtcgagcgc ttccgagagc ggcagaacgc gagcggcgcg ctggcggcgc
     1561 ggcgccgcgc gcaggaccag gcgtggatgt gggagcgcat cgaggcggga ctgaagcagc
     1621 gcttccgcag tcacccgcgc gtcaggacgg cgctgccgga gctgacggaa caggtccaga
     1681 aggggcacat cgccgcgtcg gtcgcggcgc gccggctgct cgacctgatg gattgattgc
     1741 agacggaagg aactcgacgc catgcacgag atcatcggac agctggaaca gaagcgcgcc
     1801 gcggcgcggc tcggcggcgg ccagcggcgc atcgatgcgc agcacgcgaa aggaaagctc
     1861 accgcacgcg agcgcctcga gctgctgctc gacgagggct cgttcgagga atgggacatg
     1921 ttcgtcgagc accgctgcgt cgacttcggc atggccgagc agaaggtgcc gggcgacggc
     1981 gtggtgaccg gctatgggac gatcaacggc cgcatggtgt tcgtgttcag ccaggacttc
     2041 acggtgttcg gcggcgcgct gtcggaggcg cacgcggaga agatctgcaa gatcatggat
     2101 cacgcgatga aggccggcgt gccggtgatc ggcctgaacg actccggcgg cgcgcgcatc
     2161 caggagggcg tggcgtcgct cgcgggttac gcggacgtgt tccagcgcaa cgtgctggcg
     2221 tcgggcgtga ttccgcaaat cagcatgatc atgggcccgt gcgcgggcgg cgcggtgtac
     2281 agcccggcga tgaccgattt catcttcatg gtcaaggaca gcgcgtacat gttcgtgacc
     2341 ggtccggagg tggtgcggac ggtcacgcac gaggaggtga ccgccgaaga actcggcggc
     2401 gcgatcacgc acagcacgcg ctcgggcgtg gccgacctcg cgttcgagaa tgacgtcgag
     2461 gcgctgctga tgctgcggcg gctgtacaac tacctgccgc tgaacaaccg cgaaaagccg
     2521 ccggtgcgac ggaccgacga tccggccgac cgcatggacg cgtcgctcga cacgctggtg
     2581 ccgtcgaatc cgaacaagcc gtacgacatg aaggaactga tcatcaagac ggtcgacgac
     2641 cgggacttct tcgagatcca gcccgattat gcgaagaaca tcatcgtcgg cttcgcgcgc
     2701 atggacgggc atacggtcgg catcgtcgcc aatcagccgc tggtgctcgc cggctgcctc
     2761 gacatcagaa gctcgatcaa ggcggcgcgc tttgtgcgtt tctgcgactc gttcaacatc
     2821 ccggtgatca ccttcgtcga tgtgcccggc ttcatgcccg gcaccgcgca ggaatacggc
     2881 ggcatcatca agcatggcgc gaaactgctc tttgcgttcg ccgagtgcac ggtgcccaag
     2941 gtcaccgtga tcacgcgcaa ggcctacggc ggcgcctacg acgtcatgag ttccaagcac
     3001 ctcaggggcg atgtcaacct cgcgtggcca cacgccgaga tcgccgtgat ggggccgaag
     3061 ggcgcggtcg agatcatctt ccgcgaggac aagaacgaac cggacaagct cgccgcgcgc
     3121 gaggccgaat acaaggcgcg cttcgccaac ccgttcgtcg ccggcgcgcg cggcttcatc
     3181 gacgacgtga tccagccgca cgaaacgcgc aagcgcatct gccgctcgct cgtgatgctg
     3241 cggaacaaga ggctggagaa cccgtggcgc aagcacggga acattccgct ttgagcgccc
     3301 gggggaggag cacatgttca gcaagatcct gatcgcgaac cgcggcgaga ttgcctgccg
     3361 ggtgatcaag accgcgaaga agatgggcat caggacggtg gccgtctatt ccgaggccga
     3421 ccgcgatgcg ctgcacgtcg aactggccga cgaggccgtg ccgatcggcg gcgcgccgag
     3481 ccgcgattcg tatctggtga tggacaagat catcgccgcg tgcaagcaga ccggcgcgca
     3541 ggcggtgcac cccggctacg gcttcctgtc ggagaacgag gagttcgcgc gccgcgtgga
     3601 ggacgagggc attgtcttca tcggtccgaa gcactattcg atcgcggcga tgggcgacaa
     3661 gatcgcgtcg aagaagcttg cgctcgaggc gcaggtcaa
//
LOCUS       DBNHBY010000519         2792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1320486, whole genome shotgun sequence.
ACCESSION   DBNHBY010000519 DBNHBY010000000
VERSION     DBNHBY010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1320486"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1265)
                     /locus_tag="AC16HB_12680"
     CDS             complement(66..1265)
                     /locus_tag="AC16HB_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607688.1"
                     /translation="MSAGSACPRMPASIWALGAVSLLMDISSEMIHSLLPLFMVSVLG
                     ASALAVGLIEGAAEATALIVKVFSGALSDYLGKRKLLAVIGYALGAFTKPVFALSSSL
                     ALVLTARVTDRIGKCIRGAPRDALVADLAPPEIRGAAFGLRQALDTVGAFVGPLLAVG
                     LMLLWSNDFRAVFWVAVIPGLLAVALLIFGVREPEHHADDAKRTNPIRRESLARLPRA
                     YWLVVAIGALFTLARFSEAFLVLRAQEGGMPIAWVPLVMVAMNVVYSLAAYPFGQLSD
                     RVSHAKLLMAGLVVLIAADLVLASGNHWAVVLGGVLLWGLHMGITQGLLATMIADTAP
                     ADLRGTAYGFFNLVSGVAMLAASAIAGFLWDRFGAAFTFYAGAVFCVLTLLGLALRPG
                     VGPKREV"
     gene            complement(1262..1585)
                     /locus_tag="AC16HB_12685"
     CDS             complement(1262..1585)
                     /locus_tag="AC16HB_12685"
                     /inference="COORDINATES: protein motif:HMM:NF017273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR5607689.1"
                     /translation="MSALAVWITIGGLTLVTVVTRNFFLVLGDSVRLPERVQHALRYA
                     PACALTALVAPEVLTQHGAWAITLANPKLIGAAVAVIVMLAARNTLATMAIGMAAFLL
                     ARAWT"
     gene            complement(1582..2325)
                     /locus_tag="AC16HB_12690"
     CDS             complement(1582..2325)
                     /locus_tag="AC16HB_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR5607690.1"
                     /translation="MIPLHWFGPQPAHARLRRDALIEGVWRAAPSLVATGVWGIVTGV
                     AMVNSGLTPLQAAGMSLLVFAGSAQLAALPLMAAHAPIWVVLLTALVINLRFVIFSAA
                     LYPHLRHLPLGRRVLLGYVTADFSFAVSMARWTKVPFDERGAPEHLCFLIGATASTWI
                     VWQVASLAGVALGAQIPTAWGLDFVIVVSLIALTLPMISGRPALVGVVTTGVVAVLAA
                     PLPLRLGLVVAVIVGMAAAMATDVALERS"
     gene            complement(2322..>2792)
                     /locus_tag="AC16HB_12695"
     CDS             complement(2322..>2792)
                     /locus_tag="AC16HB_12695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607691.1"
                     /translation="CGFGYPDSADVIKWHVVAMFAPGFVTGGLVRRFGTLQVMVAGYL
                     LMFGTMAIAHTGVTYWHFWGALVLLGVGWNFMFVGATTLLTTTYSAGEKAKVQGVNDL
                     TIWTTMITSSASSGALISSTGWTDLNLYALPAVAAALAAILWLLARRAPATATA"
BASE COUNT          530 a         1014 c          914 g          334 t
ORIGIN      
        1 aggccggcga cgcgaagcgc agtcgctgcc ttccacgtcg cgggcagccc gacggtgacc
       61 cggtctcaca cttcccgctt cgggcccaca ccgggccgca gcgccagccc gagaagtgtc
      121 agcacacaga acacagcgcc cgcataaaac gtgaacgcgg cgccgaagcg atcccacagg
      181 aatccggcga tcgcgctcgc cgcgagcatc gcgacgccgc tgacgagatt gaagaatccg
      241 taggcggtgc cgcgcaggtc cgccggcgcc gtgtcggcga tcatcgtcgc cagcaggccc
      301 tgcgtgattc ccatgtgcag gccccataac agcacgccgc cgagcacgac ggcccagtga
      361 ttgccggagg cgagcacgag atctgcagca atcaggacga cgagtcccgc catcagcagc
      421 ttcgcgtggc tgacgcggtc ggacagttgc ccgaacggat aggccgcaag cgagtacacg
      481 acgttcattg ccaccattac cagcggcacc cacgcgatgg gcatgccgcc ctcctgcgcc
      541 cgcagcacca agaaggcttc ggaaaagcgc gccagcgtga acagcgcgcc gatcgcaacc
      601 acgagccagt aggcgcgcgg caggcgcgcg aggctttcgc gccggatcgg gttggtgcgc
      661 ttcgcatcgt ccgcgtggtg ctcgggttcg cgcacgccga agatcagcaa cgcgaccgcg
      721 agcaggcccg ggatcacggc gacccagaac accgcgcgga aatcgttcga ccacagcaac
      781 atcaggccga ccgcgagcaa tggcccgacg aacgcgccga cggtgtcgag tgcctggcgc
      841 aacccgaacg cggcgccgcg aatctccggc ggtgcgaggt cggccaccag tgcgtcgcgc
      901 ggggcaccgc ggatgcactt gccgatgcgg tcggtcacgc gcgccgtcaa caccagcgcg
      961 aggctcgacg acagcgcgaa caccggcttg gtgaacgcgc cgagcgcgta tccgatcacc
     1021 gccagcagct tgcgcttgcc gaggtaatcg gacagtgcgc cggaaaagac cttgacgatc
     1081 aacgcggttg cctcggcagc gccctcgatc agcccgaccg ccagcgcgct cgcgccgagc
     1141 acggagacca tgaagagcgg cagcaggctg tggatcatct ccgacgagat gtccatcagc
     1201 aggctgacgg caccgagcgc ccagatgctc gccggcatgc gcgggcacgc gctgcccgcg
     1261 ctcatgtcca tgcgcgcgcg agcagaaacg cggccatgcc gatcgccatc gtcgcgagcg
     1321 tgttgcgcgc tgcgagcatc acgatgaccg cgaccgcggc accgatcagc ttgggattcg
     1381 cgagcgtgat cgcccacgcg ccgtgctggg tcagcacttc cggcgcgacg agcgcggtca
     1441 gcgcacacgc cggcgcatag cgcagcgcgt gctgcacgcg ctcgggcaga cgcaccgagt
     1501 cgcccagcac gaggaagaag ttgcgcgtga cgaccgtcac cagcgtcagg ccgccgatcg
     1561 tgatccagac cgcgagcgcg ctcatgatct ctccagtgcg acgtcggtcg ccatcgccgc
     1621 cgccatgccg acgatcacgg cgaccaccag gccgagccgc agcggcagcg gcgcggcgag
     1681 caccgcgacg acgccggtcg tcaccacgcc gaccagcgcc ggccggcccg agatcatcgg
     1741 caacgtcagc gcgatcagcg agaccacgat gacgaaatcg agcccccagg ccgtcgggat
     1801 ctgcgcgccg agcgcaacgc cggccagcga tgcgacctgc cacacgatcc acgtgctcgc
     1861 cgtggcgccg atcagaaagc acaggtgctc cggcgcacca cgttcatcga acggcacctt
     1921 ggtccagcgt gccatgctga ccgcgaagct gaagtccgcg gtgacgtacc cgagcagcac
     1981 gcgccgcccc agcggcaaat gcctcaggtg cggatacagc gctgcggaaa agatcacgaa
     2041 gcgcaggttg atcaccagcg cggtcagcag caccacccag atcggcgcgt gagcggccat
     2101 cagcggcagc gcggccaact gtgcgctgcc ggcgaatacc agcaacgaca tgccggccgc
     2161 ctgcagtggc gtgagccccg agttcaccat cgccacgccg gtcacgatgc cccacacgcc
     2221 ggtggcgacc agcgacggcg cggcgcgcca cactccttcg atcagcgcat cgcggcgcag
     2281 gcgcgcgtgt gccggctgcg ggccgaacca gtgcagcggg atcacgcggt cgccgtcgct
     2341 ggtgcgcggc gcgccaggag ccacaggatc gccgccagcg ccgccgcgac cgccggcagc
     2401 gcgtacaggt tcagatcggt ccatccggtc gacgagatca gcgcgcccga cgacgccgac
     2461 gacgtgatca tcgtggtcca tatcgtcagg tcgttcacgc cctgtacctt ggccttctcg
     2521 ccggcgctgt aggtcgtcgt cagcagcgtg gtcgcgccga cgaacatgaa gttccagcca
     2581 accccgagca gcaccagcgc gccccagaag tgccagtacg tgacgccggt atgcgcgatc
     2641 gccatcgtgc cgaacatcag caggtagccg gcaaccatga cctgcagcgt gccgaagcgc
     2701 ctgaccaggc cgccggtcac gaacccgggc gcgaacatcg cgaccacgtg ccacttgatc
     2761 acgtcggccg aatcgggata accgaagccg ca
//
LOCUS       DBNHBY010000520         1925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_168993, whole genome shotgun sequence.
ACCESSION   DBNHBY010000520 DBNHBY010000000
VERSION     DBNHBY010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_168993"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="AC16HB_12700"
     CDS             <1..1735
                     /locus_tag="AC16HB_12700"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR5607692.1"
                     /translation="QGETSACTGFSLSLVVEHLLRAARRDAPLISPYMLYSMARRYDE
                     FPGSKDEGSSLRGALKGWHKHGACADRLWTTGIELPPAPKNAEDDWWLDAVRRPLGAY
                     YRIDPRAISDMHAALNEVGIVYASAVCHAGWDVGYELKQPRVRPTSFKSPIFTIPGVP
                     AKPHDGGHAFAIVGYNEDGFLLQNSWSEAWGTRGYAILTYADWLANAMDCWVAQLGVV
                     TSEHLAIAKTNTLRTDAKGTKITLAGSNVLRDREIAPFVINMDNNGRLSNSGLFRTTP
                     DDVRAIVDIHLAAARRRWGLVGKPVDVCLYAHGGLVSEKAAAENAAQWIPLLYRNRIF
                     PVYLMWETDFLSTVIDRISDAVAGVPRAVGAREGFWSNAMKWWNERLERLLAEPGTQL
                     WGEMKQNAAAISAAADTGAGILYQHFVRSEAQKEPLRIHLVGHSAGAIVHSHIVDYLC
                     AQGMKVESVSFMAPAVRVDAFERLVAPRLADRSVRRYLQLHLTEKAEEDDPTCGPYTR
                     SLLHLVSASFEGGALTAILGMEKFWRGYGEKLKHSTAIVAPGPKSASTTHGGFDDDAL
                     TQQRVVEFIQR"
BASE COUNT          325 a          634 c          674 g          292 t
ORIGIN      
        1 ccagggcgaa acgagcgcgt gcaccggctt ttcgctgtcg ctggtggtcg aacacctgct
       61 gcgcgcggcc aggcgcgacg cgccgctgat ctcgccgtac atgctgtact cgatggcgcg
      121 ccgctacgac gagtttcccg gctcgaagga cgaaggctcg agcctgcgcg gtgcactgaa
      181 aggctggcac aagcacggcg cgtgcgccga tcggctgtgg acgacgggca tcgaactgcc
      241 tcccgcaccg aagaacgccg aggacgactg gtggctcgac gcggtgcgcc ggccgctcgg
      301 cgcgtattac cggatcgatc cgcgcgcgat cagcgacatg cacgccgcgc tgaacgaggt
      361 cggcatcgtc tacgcgagcg cggtgtgtca cgccggctgg gacgtcggct acgagctgaa
      421 gcagccccgg gtgcggccca cgtcattcaa gagtccgatc ttcacgatcc cgggtgtgcc
      481 cgcgaagccg cacgacggcg gccatgcgtt cgcgatcgtc ggctacaacg aagacggctt
      541 cctgctgcag aactcgtgga gcgaggcgtg gggcacgcgc ggctacgcga tcctcaccta
      601 tgccgactgg ctcgccaatg cgatggactg ctgggtcgcg cagctcggcg tcgtgacgtc
      661 cgagcacctc gcgatcgcga agacgaacac gctgcgtacc gacgccaagg gaacgaagat
      721 cacgctggcc ggcagcaacg tgctgcgcga ccgcgagatc gcgccgttcg tgatcaacat
      781 ggacaacaac ggcaggctgt cgaacagcgg cctgttccgc acgaccccgg acgacgtgcg
      841 cgcgatcgtc gacattcatc tggcggcagc gcgccggcgc tggggactcg tcggcaagcc
      901 ggtcgacgtg tgcctctacg cgcatggcgg tctggtcagc gaaaaggccg cggcggagaa
      961 cgcggcgcag tggattccgc tgctgtatcg caaccgcatt tttcccgtgt acctgatgtg
     1021 ggagaccgat ttcctcagca ccgtcatcga tcgcatcagc gatgcagtcg cgggcgtgcc
     1081 gcgtgccgtc ggcgcacgcg agggcttctg gtcgaacgcc atgaagtggt ggaacgagcg
     1141 gctcgagcgg ctgcttgcgg aacccggcac gcagctatgg ggcgagatga agcagaatgc
     1201 cgcggcgatc agcgccgcgg ccgacaccgg cgccggcatc ctgtaccagc acttcgtccg
     1261 ttccgaggcg cagaaggagc cgctgcgcat ccatctggtc gggcactcgg cgggcgcgat
     1321 cgtgcattcg catatcgtcg actacctgtg cgcacagggc atgaaggtcg agtccgtgag
     1381 cttcatggcg ccggcggtgc gcgtcgacgc gttcgagcgg ctggtggcgc cacgcctggc
     1441 cgatcgatcg gtgcggcgct acctgcagtt gcacctgacc gagaaggcgg aggaggacga
     1501 tccgacgtgc gggccataca cgcgctcgct gctgcacctc gtgtcggcgt cgttcgaggg
     1561 cggcgcactc acggcaatcc tcggcatgga gaagttctgg cgcggctacg gcgagaagct
     1621 gaagcattcg accgcgatcg tcgcgcccgg accgaagagc gcgagcacga cgcacggcgg
     1681 gttcgacgac gacgcgctga cgcagcagcg cgtggtcgag ttcatccaga gatgactgcg
     1741 ccgcgccgca tggccagacc gcggcgttag gactcgccga cttccagtcc ggcgagaggc
     1801 gcgtcgggcg ttcagccgat gacgcgcccg gccgcgcgcc gtagcatggt tcgacgcgac
     1861 acccgcgatc gcgcgcactt cgccgcgcgg ccttctgatc cgatgcagtc acggcagcca
     1921 gcgta
//
LOCUS       DBNHBY010000521         2003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_845534, whole genome shotgun sequence.
ACCESSION   DBNHBY010000521 DBNHBY010000000
VERSION     DBNHBY010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_845534"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /gene="argG"
                     /locus_tag="AC16HB_12705"
     CDS             <1..1313
                     /gene="argG"
                     /locus_tag="AC16HB_12705"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR5607693.1"
                     /translation="QSLPTGHKVGIAFSGGLDTSAALLWMRKKGAIPYAYTANLGQPD
                     EPDYDDIPRRALQYGAEKARLIDCRAQLVAEGIAALQCGAFHISTAGATYFNTTPIGR
                     AVTGTMLVTAMKEDDVHFWGDGSTFKGNDIERFYRYGLLANPLLKIYKPWLDQAFIDE
                     LGGRKEMSEFLIANGIDYKMSVEKAYSTDSNMLGATHEAKDLEQLDSSIRIVQPIMGA
                     AFWRDEVAIKREEVTVRFDEGQPVALNGVTFANPVELMAEANRIGGGHGLGMSDQIEN
                     RIIEAKSRGIYEAPGMALLFIAYERLVTGIHNEDTIEQYRDNGRKLGRLLYQGRWFDP
                     QAVMLRESAQRWVARAITGEVTLELRRGNDYSILNTTSPNLTYKPERLTMEKGESSFS
                     PQDRIGQLTMRNLDITDTRDKLIVYTKTGLLAPGPGALLRIEKD"
     gene            complement(1332..>2003)
                     /gene="murB"
                     /locus_tag="AC16HB_12710"
     CDS             complement(1332..>2003)
                     /gene="murB"
                     /locus_tag="AC16HB_12710"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5607694.1"
                     /translation="PGQVGAAPIQNIGAYGLELAERFESLRAWDMRERCVVQMSLADC
                     DFGYRDSVFKRELAGSRVILGVTLALPRQWQPVTSYADVANELKVRAVALPAPRDIFD
                     AVVAIRRRKLPDPALIGNAGSFFKNPVVVREKLNELLQHFPSLVSYPLPGGRFKLAAG
                     WLIDAAGLKGVTRGRAGVYEKQALVLVNRGGATGREILALAREVQEKVALKFGVTLEP
                     EPVIV"
BASE COUNT          378 a          697 c          626 g          302 t
ORIGIN      
        1 tgcagtcact cccgaccggg cacaaggtcg gcattgcgtt ttccggcgga ctcgacacca
       61 gcgcggcact gctgtggatg cgcaagaagg gggcgattcc atatgcatac accgccaacc
      121 tcgggcagcc cgacgagcct gactacgacg acatcccgcg ccgcgcgctg cagtacggtg
      181 cggagaaggc acgcctgatc gactgccgtg cgcaactggt cgccgaggga atcgccgcgc
      241 tccagtgcgg cgcattccac atctcgacgg ccggcgcgac ctacttcaac accacgccga
      301 tcggccgtgc ggtcaccggc acgatgctgg tcaccgcgat gaaggaggac gacgtccatt
      361 tctggggcga cggcagtacg ttcaagggca acgacatcga gcgcttctac cgttacggcc
      421 tgctcgccaa cccgttgctc aagatctaca agccatggct cgaccaggcg ttcatcgacg
      481 aactcggcgg ccgcaaggag atgagcgagt tcctgatcgc caacggcatc gactacaaga
      541 tgagcgtgga gaaggcgtac tccaccgact ccaacatgct cggcgccacg cacgaggcga
      601 aggacctcga acaactcgac agcagcatcc gcatcgtgca gccgatcatg ggcgcggcgt
      661 tctggcgcga cgaggtggcc atcaagcgcg aggaagtgac ggtgcgcttc gacgaaggcc
      721 agccggtcgc gctgaacggc gtcaccttcg ccaatccagt cgagctgatg gccgaggcca
      781 accgcatcgg cggcggccac ggcctcggca tgtcggacca gatcgaaaac cgcatcatcg
      841 aggccaagag ccgcggcatc tacgaggcgc cgggcatggc gttgctgttc atcgcctacg
      901 agcggctcgt gaccggcatc cacaacgagg acacgatcga acagtaccgc gacaacggcc
      961 gcaagctcgg gcgcctcctt taccaaggtc gctggttcga tccgcaggcg gtaatgctgc
     1021 gcgaatcggc gcagcgctgg gtggcgcgcg cgatcaccgg cgaggtgacc ctcgaattgc
     1081 gccgcggcaa cgactactcg atcctgaaca caacgtcgcc caatctcacc tacaaacccg
     1141 agcgtctgac gatggaaaag ggcgaatcga gcttctcgcc gcaggatcgc atcggccaac
     1201 tgacgatgcg caatctcgac atcaccgaca cgcgcgacaa gctgatcgtc tacaccaaga
     1261 ccggcctgct cgctcccggc ccgggcgcgt tgctgcggat cgagaaggac tgattgccgt
     1321 cgaccgatcg ttcagacgat caccggctcc ggttccagcg tcacgccgaa cttcagggcg
     1381 accttttcct gcacctcgcg cgcgagtgcg aggatctcgc gcccggtcgc gccgccgcgg
     1441 ttgaccagca ccagcgcctg cttctcgtac acgcccgcgc ggccgcgcgt gacacctttc
     1501 agccccgctg cgtcgatcag ccagccggcg gcgagcttga agcgtccgcc cggcagcggg
     1561 tagctgacca gcgacgggaa gtgctgcagc agttcgttga gcttctcgcg cacgacgacc
     1621 gggttcttga agaagctgcc ggcgttaccg atcagcgccg ggtcgggcag cttgcggcgc
     1681 cggatcgcga ccaccgcgtc gaagatgtcg cgcggcgccg gcagcgctac cgcgcgcacc
     1741 ttcagctcgt tggccacgtc ggcgtaactc gtcaccggct gccattgcct cggcagcgcg
     1801 agcgtcacgc cgagaatgac gcggctcccg gccagctcgc gcttgaacac gctgtcgcga
     1861 tagccgaagt cgcaatccgc gagcgacatc tgcaccacgc agcgttcgcg catgtcccac
     1921 gcgcgcagcg actcgaagcg ctccgccagt tcgagcccgt acgcgccgat gttctggatc
     1981 ggtgccgcgc cgacctgccc cgg
//
LOCUS       DBNHBY010000522         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_643149, whole genome shotgun sequence.
ACCESSION   DBNHBY010000522 DBNHBY010000000
VERSION     DBNHBY010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_643149"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="AC16HB_12715"
     CDS             complement(<1..970)
                     /locus_tag="AC16HB_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5607695.1"
                     /translation="MRLLRLLCVGFAGALAMGAATAQDIKFFRIGTGGTAGTYYPVGG
                     LIANAISNPPGSRPCSDGGSCGVPGLVATAVASNGSVANINGIQGGSLESGFTQSDVA
                     YWAYTGTGVYEGKPKVTGLRLIANLSPESIHIVARKGANIRTVADLRGKRVSLDEPGS
                     GTLVDARIILGAYGITEKDIKAEYLKPNQSGDKLRDGALDAFFFVGGYPTGAIAELAA
                     AGTGVELVPVAGPEIDKMRAQYPFFAPDTIPANTYKGVGEVKTLAVGAQWVTSDRQDA
                     NLVYELTKALWNGNSRKLLDAGHAKGKVITRENAVQGAGIPFHPGAE"
     gene            complement(1036..1386)
                     /locus_tag="AC16HB_12720"
     CDS             complement(1036..1386)
                     /locus_tag="AC16HB_12720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5607696.1"
                     /translation="MDAMSHLNNVQYFRLMEEARIQWFAQFGFPTLPTGEAPILAHAA
                     CDFVKAMTYPGTAVVKQIVTRVGRSSVEMSIVIERNDEPGVPYATGRTVIVWYDYTKG
                     QSAPWPEHVRGALE"
     gene            complement(1425..2195)
                     /locus_tag="AC16HB_12725"
     CDS             complement(1425..2195)
                     /locus_tag="AC16HB_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5607697.1"
                     /translation="MRTNLKEKIALVTGASSGLGARFARVLAANGALVVLAARREERL
                     KDLRAEIEAAGGEADVVPMDVTDIESVHAAVARVESENGPIDVLVNNSGVSTTQRLTE
                     VEPVDFDYVFDTNVKGAFFVAQAVAKRMIARSKGQPNFRGRIVNIASVAGLRVVPQIG
                     VYAMSKAAVVQMTRAQALEWGRYGINVNAICPGYIRTEINEEHWDTEAGRKLMQMLPR
                     KRVGNPEDLDGVLLLVVADESQFINGAIIAADDGLSVA"
     gene            2300..>3149
                     /locus_tag="AC16HB_12730"
     CDS             2300..>3149
                     /locus_tag="AC16HB_12730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5607698.1"
                     /translation="MQDIERESMEYDVVVVGGGPAGLSAAIRIKQRAAAAGKNVSVCV
                     LEKGSEIGAHILSGAVMDPIAINELFPDWKERGAPLNTPVTEDRFYILTERSALYNPH
                     WLLPNCFINDGYYIVSLANVVRWLGQQAEALGVEIFPGFAAAEVLYDDQGAVRGVATG
                     NMGIGKDGQPTANFQPGVELVGKYTLFAEGARGHLGKQVIAKYGLARERDPQTWGIGI
                     KEVWELDPKKHVPGLVLHSAGWPLDNSTYGGGFMYHFESNQVSLGLVVGLGYTNPHLE
                     PFQEFQR"
BASE COUNT          525 a         1085 c          999 g          540 t
ORIGIN      
        1 gctccgcgcc gggatggaac gggatgccgg cgccctgcac cgcgttctcg cgcgtgatca
       61 ccttgccctt cgcgtgtccg gcgtcgagca gcttgcgcga gttgccgttc caaagcgcct
      121 tggtcaattc gtacacgaga ttggcgtcct gtcgatccga cgtcacccac tgcgcgccca
      181 ccgccagcgt cttgacctcg ccgacgcctt tgtaagtgtt ggccgggatc gtgtcgggcg
      241 cgaagaacgg atactgcgcg cgcatcttgt cgatctcggg accggctacc ggcacgagtt
      301 cgacgccggt accggccgcc gcaagttcgg caatcgcgcc ggtcggatag ccgccgacga
      361 agaagaacgc gtcgagcgcg ccgtcgcgca gcttgtcgcc cgattggttc ggcttcaggt
      421 actcggcctt gatgtccttc tcggtgatgc catacgcgcc gaggatgatg cgtgcgtcga
      481 ccagcgtgcc cgagccgggc tcgtccagcg acacgcgctt gccacgcaga tccgcgacgg
      541 tacggatgtt cgcccccttg cgcgcgacga tgtggatgct ctccggggac agattcgcga
      601 tcagccgcag gccggtcacc ttcggcttgc cctcgtacac gcctgtaccg gtgtaggccc
      661 agtacgcgac gtccgactgc gtgaaacccg actccagcga cccaccctgg atgccattga
      721 tgttggcgac cgagccgttc gacgccaccg ccgtggcgac gagccccggc acgccgcagc
      781 tgccgccgtc gctgcagggg cgcgaaccgg gcgggttcga gatcgcattg gcgatcaacc
      841 cgccgacagg gtagtacgtg cccgcggtgc cgccggtacc gatgcggaag aacttgatgt
      901 cctgcgccgt cgcggcgccc atggccagcg cgccggcgaa gccgacgcag agcaatcgaa
      961 gcaaacgcat gactcttctc tcctgtcttg ccgttgaaaa tccgggcgcg gcgcgcgcga
     1021 gacggagaat ctacttcatt cgagcgcgcc gcggacgtgt tccggccacg gcgcggactg
     1081 gcccttcgtg tagtcgtacc acacgatcac cgtgcggccg gtcgcatacg gcaccccggg
     1141 ctcgtcgttg cgctcgatca cgatcgacat ctcgacgctc gaccggccga cgcgcgtgac
     1201 gatctgcttg acgaccgccg tgccgggata agtcatcgcc ttgacgaagt cgcacgccgc
     1261 gtgcgcgagg atcggcgcct cgccggtcgg cagcgtcgga aagccgaact gcgcgaacca
     1321 ctggatgcgc gcctcttcca tcagccggaa gtactgcacg ttgttcaggt gactcatcgc
     1381 atccatgtca ccccatcgca gcggaacttc gacgagaaac tccttcatgc cactgacaac
     1441 ccatcgtcgg ccgcgatgat ggcgccattg atgaactgcg attcgtcggc taccacgagc
     1501 agcagcacgc cgtcgaggtc ctcggggttg ccgacgcgct tgcgcggcag catctgcatc
     1561 agcttgcggc cggcctcggt atcccagtgc tcctcgttga tctcggtgcg gatgtagccg
     1621 gggcagatcg cattgacgtt gatgccgtaa cgcccccact ccagcgcctg tgccctggtc
     1681 atctgcacga cggcggcctt gctcatcgcg tagacgccga tctgcggcac gacgcgcagc
     1741 cccgccaccg acgcgatgtt gacgatgcgg ccgcggaaat tgggctggcc cttgctgcgc
     1801 gcgatcatgc gtttcgccac ggcctgtgcg acgaagaacg cacccttcac gttggtgtcg
     1861 aagacatagt cgaaatcgac cggctccacc tcggtcagcc gctgcgtggt cgacacgccc
     1921 gagttgttga cgaggacgtc gatcgggccg ttctccgact cgaccctggc gaccgcggcg
     1981 tgcaccgact cgatgtccgt cacgtccatc ggcaccacgt cggcttcacc gccggcggct
     2041 tcgatttcgg cgcgcaggtc cttcagccgt tcctcgcgcc gcgctgcgag cacgaccagc
     2101 gcaccgttgg ctgccagcac acgcgcgaag cgtgctccca gtccgctgct ggccccggtc
     2161 accagcgcga tcttttcctt caggttcgtc cgcatgttct ctcccgcgcg agcaggcgga
     2221 tgatcgcaca ccggcccctc tagaatccgc gctctacgcg cggcggcgac aatctccgaa
     2281 acacggaagc ggaggcacca tgcaggacat cgagcgcgaa tcgatggagt acgacgtggt
     2341 cgtcgtcggt ggcggccctg cgggcctgtc ggctgcgatc cgcatcaagc agcgcgcggc
     2401 cgcggccggc aagaacgtca gcgtgtgcgt gctcgagaaa ggctccgaga tcggcgcgca
     2461 catcctgtcg ggcgcggtaa tggatccgat cgcgatcaat gaactattcc ccgactggaa
     2521 ggagcgcggc gcgccgctga acacccccgt caccgaggac cgcttctaca tcctcaccga
     2581 aaggagcgcg ctgtacaacc cgcactggct gctgccgaac tgcttcatca acgacggcta
     2641 ctacatcgtc agcctcgcca acgtggtgcg ctggctcggc cagcaggctg aggcgctcgg
     2701 cgtggagatc tttcccggct tcgcggccgc ggaggtgctg tatgacgatc agggcgccgt
     2761 gcgcggcgtg gcgacgggaa acatgggcat cgggaaggac gggcagccca ccgccaactt
     2821 ccagcccggc gtcgaactcg tgggcaaata cacgctgttc gccgagggcg cgcgcggcca
     2881 cctcggcaag caggtgatcg cgaagtacgg gctcgccagg gaacgcgatc cgcagacctg
     2941 gggcatcggc atcaaggaag tgtgggagct cgacccgaag aagcacgtgc ccggactcgt
     3001 gctgcacagc gccggctggc cgctcgacaa ttcgacctat ggcggcggct tcatgtatca
     3061 cttcgaaagc aaccaggtgt cgctcgggct cgtcgtcggt ctcggctaca ccaatcccca
     3121 tctcgaaccg ttccaggaat tccagcgct
//
LOCUS       DBNHBY010000523         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_643182, whole genome shotgun sequence.
ACCESSION   DBNHBY010000523 DBNHBY010000000
VERSION     DBNHBY010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_643182"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..657)
                     /locus_tag="AC16HB_12735"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="AC16HB_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517408.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(667..>1683)
                     /locus_tag="AC16HB_12740"
     CDS             complement(667..>1683)
                     /locus_tag="AC16HB_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR5607699.1"
                     /translation="GKGIAWNTQDEVQALKDLNGVVAEEGATKGLAKIDTDIDAAEVI
                     LMLAPETNGHVAVKAWDALGKITGVDHKHLALHREDEKIRFRDIQAQPRKIISSPTWS
                     GLESEKVSYNAGYTNVHELIPWRTLSGRQQYYLDHAWMLAFGEGFCVYRPPVDLKTLD
                     IKGSRPNGNAEVVLNFITPHQKWGIHSTYTDNLIMLTLNRGGPVVWLSETDAKAAGIV
                     DNDWIELSNINGAIAARAVVSQRVNAGMCLMYHAQEKIVNTPGSEITGQRGGIHNSVT
                     RAVLKPTHMIGGYAQLAYGFNYYGTIGTNREEFVIVRKMKTVDWMDTPAPDAMAQAAA
                     AANA"
BASE COUNT          242 a          556 c          518 g          367 t
ORIGIN      
        1 ccagccgcgg cacttgtcct ggtcgatcag cacgatgccg tcttcctcgc gcttgtagat
       61 cgagccagat gggcacgacg ccacgccggt cgggttcagg cagtgctagc acaggcacgg
      121 caggtacatc atgaaggtct tctcgaactc gccgtagacc tccttctcca tcgcttcgaa
      181 gttcgcatcg cgcgatcgct tggcgaactc gccgcccagg atctcctccc agttcgggcc
      241 ccactcgatc ttctccatcc gctggcccgt caccagcgag cgcggacgcg cggtcggtgc
      301 tgccttcatc tcgggcgcgt tgtgcaggtg ctcgtagtcg aagtcgaacg gctcgtagta
      361 gtcgtcgatc tcgggcaggt tcggattggc gaagatgttc atcaggatgc gcgcgcgtcc
      421 gccctggcgc ggctcgatcc ggccgttgcc cttgcgcacc cagccgcctt tccatttctt
      481 ctggttctcc cattccttgg gatagccaac gccgggcttg gtctcgacgt tgttgaacca
      541 cgcgtactcc atgcccggcc ggctcgtcca cacgttcttg caggtgaccg agcaggtgtg
      601 gcaaccgatg catttgtcca ggttcaggac catgccgatc tgtgctctga ctttcatggt
      661 gtcctctcaa gcattggccg ccgcagccgc ctgcgccatc gcatcgggcg ccggggtgtc
      721 catccagtcc acggtcttca tcttgcgcac gatcacgaac tcttcgcggt tggtgccgat
      781 ggtgccgtag tagttgaagc cgtacgcgag ttgcgcatag ccaccgatca tgtgcgtagg
      841 cttcaggacc gcgcgcgtga ccgagttgtg gatgccgcca cgctggccgg tgatttccga
      901 gcccggcgtg ttcacgatct tctcctgcgc gtggtacatc aggcacatgc cggcattgac
      961 gcgctggctg accaccgcgc gcgcggcgat cgcgccgttg atgttggaca actcgatcca
     1021 gtcgttgtcg acgatgccag ccgctttcgc atcggtctcc gagagccaca ccacggggcc
     1081 gccgcggttc agcgtcagca tgatcaggtt gtcggtgtag gtggagtgga taccccactt
     1141 ctggtgcggc gtgatgaagt tcagcacgac ctcggcattg ccgttcggcc gtgagccctt
     1201 gatgtcgagc gtcttcaggt cgaccggcgg gcggtacacg cagaacccct cgccgaacgc
     1261 gagcatccac gcgtggtcga ggtagtactg ctgccgtccc gacagcgtgc gccacgggat
     1321 cagctcgtgc acgttggtgt agccggcgtt gtaggagacc ttctcgcttt ccaggcccga
     1381 ccacgtcggc gaactgatga tcttgcgcgg ctgcgcctgg atgtcgcgga agcggatctt
     1441 ctcgtcctcg cgatgcagcg ccaggtgctt gtggtcgacg ccggtgatct tgccgagcgc
     1501 atcccacgcc ttgaccgcca cgtggccgtt ggtttccggc gcgagcatca ggatcacctc
     1561 ggcggcgtcg atgtcggtgt cgatcttcgc cagtcccttg gtggcgcctt cctcggcgac
     1621 cacgccattg aggtccttca gcgcctgcac ctcgtcctgc gtgttccacg cgatgccctt
     1681 gcc
//
LOCUS       DBNHBY010000524         5747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_101793, whole genome shotgun sequence.
ACCESSION   DBNHBY010000524 DBNHBY010000000
VERSION     DBNHBY010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_101793"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..374
                     /locus_tag="AC16HB_12745"
     CDS             51..374
                     /locus_tag="AC16HB_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HIR5607700.1"
                     /translation="MATRKLSELAKTIRSKNAGTDKITFDIIFRERDAYETVKSSGAI
                     NRDRMARLLNIDESRITDFVEYDEAYAIKFTILRERPSGSAGDPDIFGSQQYAPLLDI
                     EISTF"
     gene            complement(982..2502)
                     /locus_tag="AC16HB_12750"
     CDS             complement(982..2502)
                     /locus_tag="AC16HB_12750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5607701.1"
                     /translation="MTVIDANGKFLMPAMIDAEGHFNRPVDALDQLLVRDAAVCGEGT
                     DKGQTLRASTAEVMSAFVTKGLLTEVDTHGLRIGSGWNPHAAGGRYPLAPSANYEKHL
                     RFGVATVLDMSAYPWPANYVRRSRDRWRAPRGVEDADLQREFLVYADLFTAGMWAAPP
                     MLHFGYYGVDPVYNVQPEGPWKSSDVRAWVRRRVAEGSDHVKVFYDHWRGRDVPKISP
                     ATLKALVQAAHEQKLRVFVHNHSEEASEALMDAGADVNIHLPGGSGFGGPPISDEFVA
                     RFARLIHAIVPTLTPFVSGCRNPFSPSNRLTHALSQHAPGMSFMTAAVETADVLPYIN
                     AFDEARLAACEGLDDFERNFRNLAALFDAGVTLMVGADAGDFSPQVEGLSVHYEAWVM
                     SEALKRYSKTVTGRAANLAVLKAATSNAARVYGLHRENGKRPTADPRGFVKPGYRADL
                     LLLHASPMDDMLNTLKIDRVFKAGYDANRQMVRPECASGDCESRRVLREIEERSCR"
     gene            2440..2841
                     /locus_tag="AC16HB_12755"
     CDS             2440..2841
                     /locus_tag="AC16HB_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607702.1"
                     /translation="MTLGIDHCRHQELAVRVDDGHGRIHTRGVVVRGAQPLDPPTAYE
                     HAAAIDDALAIEDGRAADQERIRPAQRFVDAAQGGRGGLRDCGCVDQGARSDKDRSED
                     AWQPIHRDIPPTILRAATILERPNGAVKTDP"
     gene            3068..3196
                     /locus_tag="AC16HB_12760"
     CDS             3068..3196
                     /locus_tag="AC16HB_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607703.1"
                     /translation="MAKGQLRSNKEKKKPKQSKKPTSPAVPFGSTQNKTPPPAPAK"
     gene            3664..4416
                     /locus_tag="AC16HB_12765"
     CDS             3664..4416
                     /locus_tag="AC16HB_12765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607704.1"
                     /translation="MTEVYEDVQPPAESTAGTAVFTARGLTKNYRMGEVEVHALRGVD
                     LDIYAREFVVLLGPSGSGKSTLLNILGGLDTPSGGTAMWRDHDLTHADEAELTRYRRE
                     HVGFVFQFYNLIPSLTARENVALVTEMSRDPMQPEDALALVGLGDRLDHYPSQLSGGE
                     QQRVAIARAIAKRPDVLLCDEPTGALDYATGKLVLAAIAKINVELGTTAAVITHNAPI
                     ARIADRVLRMADGRIAEVTVNSAKVSPEAITW"
     gene            4410..>5747
                     /locus_tag="AC16HB_12770"
     CDS             4410..>5747
                     /locus_tag="AC16HB_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607705.1"
                     /translation="MVRMLDRKLLRDVWHLRGQVLAIALVVAAGIVGYCGSLATYDSL
                     RWLQAGYYETSRFAHVFAQGKRAPLAVADRLLELPGVAEVETTVVFDVMLDVQDEIAP
                     LTGRMIALPAHGLPRINRLTLRRGAWIDVPRSNQVLVNETFANAHKLAPGDRLTALLN
                     GKRETMQVVGIVLSPEYVFPGRGGLGDEKSFGILWIGRERLAAAFNMEGAFNSVAVRL
                     ARGAAEPGVIAALDRVLDRYGFTGAYGRTDQLSHKALTQEISQWRVYGTALPVVVLGV
                     AVFLLNVALARQIGTQRSQIAALKALGCDDRAIGLHYLKFVLVIVLIGGPIGVAGGYY
                     FGYAVAQLYARFFRFPDFEFRMLAWIPLSAVLLSMFAAIAGVVTALRRVVRLPPAEAM
                     RPAAPATFRPTLMEAIGLGHLYSPAVRMIVRDIEREPLRAILTTFGIATAVAVM"
BASE COUNT          981 a         1858 c         1900 g         1008 t
ORIGIN      
        1 gatccgcttg agctctttcc gtgtcacgtg gccgaggtcg ggggatgacg atggcaaccc
       61 gaaaactctc cgaactggcg aagacgatcc gcagcaagaa cgcgggcacc gacaagatca
      121 cgttcgacat cattttccgc gagcgcgatg cgtacgagac cgtcaagagc agtggcgcga
      181 tcaaccgtga caggatggcg cgcctgctca acatcgacga atctcgaatc accgattttg
      241 tcgaatacga cgaggcctat gcgatcaagt tcacgatcct gcgcgaacgc ccgagcggaa
      301 gcgccggaga tccggacatc tttggttcac agcaatacgc gccgctgctc gacatcgaga
      361 tctcaacgtt ctgagcgcca cgactgaagg tactccgaca tcggacggta cgccggctgg
      421 cagatcggcg aatcatgtgc atgtgtcgaa tccgattgtg cacgtgcagg atgaaacaag
      481 cgtctgtctc ctctcccggt tagggtcgat tgcggagtct ggctgaggtg cgcgaagcga
      541 aggctctgac cattttgtca ggcgcgggaa cgcacctcgt cgaagtcgag gcggtgcctt
      601 cttacggcca gaactggcca tgcgcgcgct cacaaaacga cgttcggaat gacgggtctc
      661 aaagcgcaac gcgccgttac ccgacgtgag gcattcggtg acgcaatttc gatcgctgac
      721 agccgtagac ttccaacggc agtcagcaag aaacaccgcg cgagcgagtg ttcgtcgcaa
      781 gatcggactg gcgcaggcga cccgtcgagg cgttcgggcc tgccggcgtg aatctccccg
      841 gtagagcttc gagcggactc tgcgctgagg ttcttcgtcg accgtaccct ggcatatctt
      901 gaaatccacc cgcggtcggt tgtctgcact tcttcttgtt gatgcgtaag cgtcgaccca
      961 cgcgcaagcg acgcttgctc ttcagcgaca gcttcgctct tcgatttcgc gcagcacgcg
     1021 gcgagactcg caatcgccgc tggcgcattc cggtcgaacc atctggcggt tcgcgtcata
     1081 acccgccttg aacactcggt cgatcttcag cgtgttgagc atgtcgtcca tcgggctggc
     1141 gtgcagcaga agcagatcgg ctcgataccc gggcttcacg aatcctcgcg gatcggccgt
     1201 cgggcgcttc ccgttctcgc ggtgcaggcc atagacgcgc gcggcattgg acgtggcggc
     1261 cttgaggact gcgaggtttg cggcacgacc cgtcaccgtc ttcgagtatc gcttgagggc
     1321 ctcgctcatc acccaggctt cgtagtgaac gctgagaccc tcgacctgcg gactgaagtc
     1381 gccggcgtcc gcgcccacca tcagcgtgac gcccgcgtcg aaaagcgcag ccaggttacg
     1441 aaagttgcgc tcgaaatcgt ccaggccttc gcacgccgcc aggcgcgcct cgtcgaatgc
     1501 attgatgtac ggcagcacgt cggcggtctc gacggcagcg gtcatgaagc tcatccccgg
     1561 cgcgtgctgc gacagtgcgt gcgtcagacg attggaagga ctgaaaggat tgcggcaacc
     1621 gctgacgaac ggcgtcaggg tcggaacgat cgcatgtatg agccgcgcga atcgtgcgac
     1681 gaactcgtcg ctgatgggcg gaccgccgaa gcccgacccg cccggcagat ggatgttcac
     1741 gtcagcgccc gcgtccatca gcgcctcgga cgcttcctcg ctgtggttgt gcacaaagac
     1801 gcgcagcttc tgctcgtgcg ccgcctgcac cagggccttg agcgtggccg gcgagatctt
     1861 cggcacgtcg cgaccacgcc aatgatcgta gaagaccttg acgtggtccg atccctccgc
     1921 gacgcgtcgc cgcacccagg cccgcacgtc ggaagatttc cagggccctt cgggctgcac
     1981 gttgtaaacc gggtcaactc cgtagtagcc gaagtgcagc atcggcggtg ccgcccacat
     2041 gcctgcagta aacaggtcgg cgtagacgag gaattcacgt tgcaggtcgg catcttcgac
     2101 gccacgggga gcgcgccaac ggtcgcgcga tcgccgcacg tagttggcgg gccacgggta
     2161 ggcgctcatg tcgagcacgg tggcgacgcc gaagcgcaga tgtttctcgt agtttgcgct
     2221 gggcgccagc ggataacggc ctccggccgc atgggggttc cagcctgatc cgattctcag
     2281 accgtgcgtg tcgacctcgg tgagcaggcc cttggtgaca aaggccgaca tgacctcggc
     2341 cgtggaagcc ctcaacgtct gccccttgtc cgttccctcc ccgcacactg ccgcgtcgcg
     2401 aaccaggagt tggtccagcg cgtcgacagg tcggttgaag tgaccctcgg catcgatcat
     2461 tgccggcatc aggaacttgc cgttcgcgtc gatgacggtc acggtcggat ccacacacgc
     2521 ggcgtcgtcg tccgcggcgc acagcctctc gatccaccca ccgcgtacga gcacgctgcc
     2581 gcgatcgatg atgcgctcgc catcgaagat ggtcgcgccg cggatcagga acgaattcgg
     2641 ccggcgcagc gcttcgtcga tgccgcgcag ggaggccgag gcggactgcg cgattgcgga
     2701 tgtgtcgacc agggcgcccg aagtgacaag gaccgcagcg aggatgcgtg gcaaccgatt
     2761 catcgtgaca ttcctcccac gatcctgcgg gcggcaacga ttctcgagcg gcccaatggt
     2821 gcggtcaaga ctgacccatg actcgagcgg catatccccg gacgacggtc gcgggacatt
     2881 cgatgatgtg ggcacgcggc caaatcggtc agcggcaggt tgccgggttg acgggtcaat
     2941 tgaccagaaa tatggctgcc agccgctata ggtcgtactg cgaagttcag tcggtgttgc
     3001 gtttccagtc gcaaagtcgg atagtccccg gcgtcgagcg cgcgcgcatc gttcggggag
     3061 aattcagatg gccaagggtc agcttcgaag caacaaggaa aagaagaagc ccaaacagtc
     3121 gaagaagccg acgagcccgg cggtgccttt cggttcaacc cagaacaaga cgccgccgcc
     3181 cgcgccggcc aagtagtctc ggatcgttcc ggtcgaaccg cgacgcgtcg ccgcgacgct
     3241 tcggtcgcgt acccggtcga acagaaccgg caccatctgc gggtcgacgc aggcgcgcgt
     3301 tgctcacgcg tccgcaagcg cattgcgatt gcgcgtcctg tcccgctgcg gcatcggacc
     3361 tcgcccgccg ccgggaacga actccgtcgg ctcgcagttc ccgcattcgc gcgcacgttg
     3421 ccgcaggggc gccatctgcc cacgctgcgt ctgatgagcc tcaagtcgct gctccggcaa
     3481 gggatcagca tcttcccgtt tgccgcgcca cacagtgcgc atgccgcggt tcagcgcaat
     3541 ggagcctctt gaggtcttca cccgctccac tgcggcgctc gggccgctgc ggtcggtgca
     3601 atgcgtgtgc gcggaatggc cgaccctgca gccagtttgg cgcggtgtcg ggtcggagcc
     3661 gcaatgaccg aggtctacga agacgtccag cctcccgccg aaagcacagc gggcacggcc
     3721 gtcttcaccg cccgcggcct gaccaagaac taccggatgg gcgaggtcga ggtgcacgcg
     3781 ctgcgtggcg tcgatctcga catctacgcg cgcgaattcg tcgtgctgct gggcccgtca
     3841 ggcagcggca agtcgacgct gctgaacatc ctcggcggcc tcgatacgcc gtccggcggc
     3901 accgcgatgt ggcgcgacca cgacctcacg cacgccgacg aggcggagtt gacgcgctac
     3961 cggcgcgagc atgtcggctt cgtgttccag ttctacaacc tgatcccgtc gctcaccgcg
     4021 cgcgagaacg tcgcgctggt gaccgagatg tcgcgtgacc cgatgcagcc cgaggacgcg
     4081 cttgcactcg tggggctcgg cgaccggctc gaccactatc cgtcgcagct ttcgggtggc
     4141 gagcagcagc gcgtggcgat cgcgcgcgcc attgccaagc ggcccgacgt cctgctgtgc
     4201 gacgagccaa ccggcgcgct cgactacgcg accggcaagc tcgtgctcgc cgcgatcgcg
     4261 aagatcaacg tcgaactcgg cacgaccgcc gcggtgatca cgcacaacgc gccgatcgcc
     4321 cgcatcgccg atcgcgtgct gcggatggcc gacgggcgca tcgccgaggt gacggtgaac
     4381 agcgccaagg tgtcgccgga ggcgatcaca tggtgaggat gctcgaccgc aagctgctgc
     4441 gcgatgtctg gcatctgcgc ggacaggtgc tcgcgatcgc actggtggtc gcggcgggca
     4501 tcgtcggtta ctgcggttcg ctggcgacct acgactcgct gcgctggctg caggcgggct
     4561 attacgagac ttcgcgcttc gcgcatgtct tcgcgcaggg caagcgtgcg ccgctggcgg
     4621 tggccgaccg gctgctcgag cttcccggcg tggccgaggt cgagaccacg gtcgtgttcg
     4681 acgtgatgct cgacgtgcag gacgagatcg cgccgctgac cgggcgcatg atcgcgctgc
     4741 ccgcgcacgg gttgccgcgg atcaaccggc tgacgctgag gcgaggcgcg tggatcgacg
     4801 taccgcgttc caaccaggtg ctggtcaacg aaaccttcgc gaacgcgcac aagcttgcgc
     4861 ccggcgatcg cctgaccgcg ctgctcaatg gcaagcgcga aacgatgcag gtggtcggca
     4921 tcgtgctgtc gcccgagtac gtttttcccg gccgcggcgg cctgggcgac gaaaagagtt
     4981 tcggcatctt gtggatcggc cgcgaacggc tggcggcggc cttcaacatg gaaggtgcat
     5041 tcaactcggt ggcggtccga ttggcgcgcg gcgccgcgga gccgggcgtg atcgcggcgc
     5101 tcgaccgcgt gctcgaccgc tacggtttca cgggtgcata cggtcgcacc gatcagcttt
     5161 cgcacaaggc gctgacccag gagatctcgc aatggcgggt ctacggcacg gcgctgccgg
     5221 tcgtcgtgct cggcgtggcg gtgttcctgc tcaacgtggc gctcgcgcgg cagatcggca
     5281 cgcagcggtc gcagatcgcc gcactgaagg cgctcggctg cgacgaccgt gcgatcgggc
     5341 tgcactacct gaagttcgtg ctcgtgatcg tgctgatcgg gggaccgatc ggcgtcgcgg
     5401 gcgggtacta cttcggctac gcggtcgcgc agctgtatgc gcgcttcttc cggttccccg
     5461 atttcgagtt tcgcatgctg gcgtggatcc ctttatccgc cgtgctgctg tcgatgttcg
     5521 ctgcgattgc aggcgtggtc acggcgctgc ggcgcgtggt gcggttgccg ccggcggaag
     5581 cgatgcggcc ggccgcgccg gcgaccttcc ggccgacgct gatggaggcg atcgggctcg
     5641 gtcacctgta ctccccggcc gtgcggatga tcgtgcgcga catcgagcgc gagccgctgc
     5701 gcgcaattct gacgacgttc ggcatcgcca ccgcggtggc ggtgatg
//
LOCUS       DBNHBY010000525        12564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_373323, whole genome shotgun sequence.
ACCESSION   DBNHBY010000525 DBNHBY010000000
VERSION     DBNHBY010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_373323"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="AC16HB_12775"
     CDS             <1..821
                     /locus_tag="AC16HB_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR5607706.1"
                     /translation="ATGALLALSGAILQALLRNPLADPYVLGVSGGAAVAALVALALG
                     AGALGMQFASIAGAAVTMLLLFVFARRGLFARAASHAARAEDGTTHVLLTGVMIAAFC
                     AAALSLLLSLAPDGRLRTMVFWLLGDLGGATSLPSATIALALLVLALVVSEKLAAPLN
                     LLLRGDVQAFTQGVDVERTRRALVIVAALATGAAVTLAGAIGFVGFVAPHLMRRVIGN
                     DQRVLLPAAALAGGVLVVVADTVARTAAAPLQIPVGVLTAMIGVPIFLWLLRTR"
     gene            818..1573
                     /locus_tag="AC16HB_12780"
     CDS             818..1573
                     /locus_tag="AC16HB_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607707.1"
                     /translation="MSTLLECRQVDLRAGRRELVRALSLRIDAGERWAVLGPNGAGKS
                     TLLAALAGVRAPNGGEILLAGRALHHWSVEQMAEQRALVTDRWHDPFSASVLDTVLTA
                     RYWLGAEDDAGVQIARGWLAEFDCAELIDHDVTSLSRGERQRVALATALAQDTPLVLL
                     DEPIAHQDPRHQGLVLDRLARAPGRTLVASLHDMNAAARFATHALLLTGRGAWSAGPA
                     ADMLSAPRLSELFETDVITMATPDGARVFAIGG"
     gene            complement(1574..2014)
                     /locus_tag="AC16HB_12785"
     CDS             complement(1574..2014)
                     /locus_tag="AC16HB_12785"
                     /inference="COORDINATES: protein motif:HMM:NF022479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR5607708.1"
                     /translation="MKDQLDIIAHVIQLAIAPVFLLTGIGALLGVMMQRVARIIDRAR
                     TVEDELGTASDRRAHALHDELRTLSRRLRLVNRAVSLCVLAALLVCGVIGGLFITAYY
                     AWSPDLTRIVATVFAAALLSLIAGLLFFLREVYIATATLRFGVK"
     gene            complement(2011..2889)
                     /locus_tag="AC16HB_12790"
     CDS             complement(2011..2889)
                     /locus_tag="AC16HB_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR5607709.1"
                     /translation="MIRALAALLVLLIGADASAAQPVAAIDDSQARIELARPAQRIVS
                     LAPHITEQLFAIGAGDRIVATTEYADYPPAARKLPRVARAHSVDLERVAAARPDLIVI
                     WGSGFPPAIVGSVRRLGVPVFIDEPGSLESIATSLERLGVLTGSRTAPQVAAEFRARI
                     AALRARYSTRAPVRVFYQIWAQPLMTLAGTHVINEAIRLCGGRNIFEPLTAIAPQVSI
                     EAVVVADPQVIVTAEPGARPSDALDLWRRFDGVSAVRNGQLVTLDADRINRHGPRIVD
                     EITVLCERLDAARQSR"
     gene            complement(2886..3326)
                     /locus_tag="AC16HB_12795"
     CDS             complement(2886..3326)
                     /locus_tag="AC16HB_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5607710.1"
                     /translation="MTFGGFERGQSTSQPMAEINVTPLVDVMLVLLVIFIITAPLLTY
                     ALKLDLPEVKSTPVSPSTEPVRLSIDGAGAIYLNEQPLEGEALRTRLAELAARTPPPE
                     VLLRVDKAARFERAALVMSLAQEAGLTRIGFVTEPPQATGTPRP"
     gene            complement(3326..4009)
                     /locus_tag="AC16HB_12800"
     CDS             complement(3326..4009)
                     /locus_tag="AC16HB_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5607711.1"
                     /translation="MNPSLGLSHFWSQADAIIRLTAYILLAMSVASWCVILWKAWAWL
                     RARRAARALEQFWAARSIDEAIALLKPADAEAIFVPLATSAAGAAAPPPGTQSLSALI
                     DRSELITRALRQRINEAAARLESGLTFLASVGSSAPFVGLFGTVWGIYHALLNIASSG
                     SVAIDKVAGPVGEALVMTAFGLVVAVPALLGYNAFTRVNRMLLAQLDGFAHDLHAFLT
                     VGGRFQPGT"
     gene            complement(4006..4806)
                     /gene="dapB"
                     /locus_tag="AC16HB_12805"
     CDS             complement(4006..4806)
                     /gene="dapB"
                     /locus_tag="AC16HB_12805"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR5607712.1"
                     /translation="MTLKLAIAGAGGRMGRMLIEAALGADDLAIAAALDRAGSPDLGR
                     DCAEFLGRATGVRIIDDLAALRGADVLIDFTRPEGTLEHLRACADAGVKMVIGTTGFD
                     DAGRAAIHAAAEGIAIVFAPNMSVGVNATFKLLEVAAQILSSGYDIEIIEAHHRHKVD
                     APSGTALKMGEVVATALGKKLGDVAVYAREGVTGERKPGTIGFASVRGGDIVGDHTVL
                     FAGIGERIEITHRSASRMTYAHGALRAARFLAQRSRGLFDMHDVLGLR"
     gene            complement(4803..5264)
                     /locus_tag="AC16HB_12810"
     CDS             complement(4803..5264)
                     /locus_tag="AC16HB_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR5607713.1"
                     /translation="MKDTLKTAVTVLLISATALLAGCESMRSWTPTFIQPYRPDVQQG
                     NVVTKEMVEQLRPGMTRDQVRFLLGTPLVASMFHQDRWDYIYHLKRGKGTEEQRRRLT
                     VLFKDNRLDTFTADEMPPETMADNLILGRNPKALPHAPPKEPPAQINLPQK"
     gene            5324..5785
                     /gene="fur"
                     /locus_tag="AC16HB_12815"
     CDS             5324..5785
                     /gene="fur"
                     /locus_tag="AC16HB_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR5607714.1"
                     /translation="MQQQRSGTASSAELKSMGLKATLPRMKILEIFQRSSATQGVRQL
                     SAEDVYKALLAENLDVGLATVYRVLTQFEQAGLVKRSHFEAGRAVFELDEGKHHDHLV
                     CVTCGRVEEFVDAEIEKRQQQIAQTKGFKLQEHSLALYGECTRLNCPHRPG"
     gene            complement(5798..7447)
                     /gene="recN"
                     /locus_tag="AC16HB_12820"
     CDS             complement(5798..7447)
                     /gene="recN"
                     /locus_tag="AC16HB_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5607715.1"
                     /translation="MLLGLSIRNFVIVDVLDLEFAPGFTVLTGETGAGKSILIDALLL
                     ALGERADAGVVREGATRADINAEFRVDGAAAAWLAANELAAEEDTVLVRRTVDAAGRS
                     KGFINGTAVTQAQLRDLGELLVDVHGQHAHQSLLRPAAQLALLDEHGGLTAAARAVAT
                     AYAAWRREREARERSEQMAGAAALEQERLRWVVDELAALAPQAGEWETIEAEHMRLSH
                     AASLLEGARGAVDALSEGDGAALERIDAVLSRLAPLAGFDARLAPIAATLDAARVQVD
                     DAARELNHYLAKTEVDDARLAELDARVTALHAAARKFRCAPADLAALLAQSQEKLNAL
                     AAASDLDGLKKRETDAEQAYLKLARALSKQRAKAAANMAREVTRAMQDLAMPGGRFDI
                     ALTAGEPAAQGLERVEFLVAGHAGVAPKPLAKVASGGELARISLAISVIAATATPVAT
                     LIFDEVDAGIGGAVAETVGRLLQQLGRERQVLCVTHLPQVAARGDQHCVVSKVEADDG
                     RPVSRIALLDRRTRVEELARMLGGLQITDTTRKHAREMLAA"
     gene            complement(7458..8360)
                     /locus_tag="AC16HB_12825"
     CDS             complement(7458..8360)
                     /locus_tag="AC16HB_12825"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041679527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR5607716.1"
                     /translation="MASPFKHVAIFGKPRADAARPALVSTLRDVARIVELAGQTVMFD
                     AATRDAASLDPYRGYTPEQIGATADVAVVLGGDGTMLGIARELAPFNVPLIGINLGRL
                     GFITDIAVAQLSEVLAAMLAGEYETDRRALLEAEVRRAGAVVFRARALNDVVVSRGVT
                     GGMVEFTVRVDGVTMYNQRADGVILATPTGSTAYALSASGPILHPSLAGFVLVPVAPQ
                     TLSNRPIALPDSVAVEIEVTDVRDAGAHFDMQAFTSLASGDIVCARRGRNVVTLLHPV
                     GYNYFATLRQKLHWNIMPADAHRN"
     gene            8418..9431
                     /gene="hrcA"
                     /locus_tag="AC16HB_12830"
     CDS             8418..9431
                     /gene="hrcA"
                     /locus_tag="AC16HB_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR5607717.1"
                     /translation="MLDDRARLLLKTLIERYIAEGQPVGSRSLSKISGLELSPATIRN
                     VMADLEEMGFVSSPHTSAGRVPTPRGYRLFVDTLLTVQPMEQQQAAAIEGRLRVAEPQ
                     RAILTAAQLLSNLSHFAGVVMTPRRASSFRQVEFVRLGERRVLLIIVTPEGDVQNRIL
                     LTDHAYSPAQLVEAANILNEHFAGLSFDEARMRLHGDLARLREDIAQLMEAAVQAGSQ
                     AAEDSEPVVVSGERNLLGVADLASNLDHLRKLFDLFEHKTRLMQLLDVSARAHGVQIY
                     IGGESALVPLDEMSMVVAPYEVDGQVVGTLGVIGPTRMAYERVIPIVDITARLLSSAL
                     SRD"
     gene            9445..10557
                     /gene="hemH"
                     /locus_tag="AC16HB_12835"
     CDS             9445..10557
                     /gene="hemH"
                     /locus_tag="AC16HB_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR5607718.1"
                     /translation="MRFLREPAYAHAAAARTAVLLVNLGTPDAPEPRAVRRYLREFLS
                     DPRVVEIPRALWLPILHGIILNVRPRKSAAKYASIWTPAGSPLRVYSEQQSVLLRGYL
                     GHRGLDVDVALAMRYGQPSIGAVLGGLREKRVERLLVLPLYPQYSATTTATVFDAVHA
                     VLARTRNVPEVRWVRHFHDEPRYVEALKTNVLAYWKKQGRAQDAGGKLLMSFHGVPRR
                     SLDLGDPYHCECQKTARLLAEALHLSKDEYAVTFQSRFGRAEWLRPYTAATLHELGAQ
                     RARRVDVICPGFVADCLETLEEIAIEGRQEFMSAGGSEYHYIPCLNDAPAFIEALADV
                     VAANVQGWPVDRAQRGAREQQAQAGAARARLLGAAR"
     gene            complement(10589..10816)
                     /locus_tag="AC16HB_12840"
     CDS             complement(10589..10816)
                     /locus_tag="AC16HB_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607719.1"
                     /translation="MRDDDTFEFESGERGSSQLPAVLMAAAAIAAILLVNSTVLAPAV
                     AATARAAPAAPSVTSVPDGEAATPAASALRT"
     gene            complement(10956..11705)
                     /locus_tag="AC16HB_12845"
     CDS             complement(10956..11705)
                     /locus_tag="AC16HB_12845"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein"
                     /protein_id="HIR5607720.1"
                     /translation="MPTGTPMNKLHPLIATAAVAVTAVSVLGAVVLVTNQVSAQHTPA
                     PAAVPAALPTEGASAPAAATPAAPAPRALPASRAHATAAPATPKPPVQLAAAAPAPLP
                     PGVLTQMPAVPSVTAQAPVCTSCGTVTSVREVKAPGEGTGIGVIAGGVLGGVIGNQFG
                     RGGGKDASRILGAIGGAVAGHQIEKQARATVRYEITVRMEDGSLRTLTRAINPGVKAG
                     DRVRVEGDDLRAADGAPLAPTPQFTPERGSA"
     gene            complement(11821..>12564)
                     /locus_tag="AC16HB_12850"
     CDS             complement(11821..>12564)
                     /locus_tag="AC16HB_12850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5607721.1"
                     /translation="RDWGEQWASHGFVVIHVQHPGSDESVWRGAEKPARALRAAAGAQ
                     QFIDRVRDVQFVLDELERRRQVGDPVAARIDPARIGLSGHSFGAITTQAIAGEDFGAR
                     ARAQAVADARPRAFIAFSPSARSRSDAQRFAPVVRPFFSVTGTRDGEVGMGLGVPPAQ
                     RLLPFEGMPAGDKYLLNLVGADHMLFNGGERWRGAAVDAALDARHKRVVRATTTAFWL
                     AYLDHDAAARRWLANAQRYVGQAGEFRVK"
BASE COUNT         1824 a         4389 c         4456 g         1895 t
ORIGIN      
        1 tcgccaccgg cgcgctgctg gcgttgtcgg gcgcgatcct gcaggcgctg ctgcgcaatc
       61 cgctggccga cccgtacgtg ctcggcgtct cgggcggcgc ggcggtggcg gcgctggtcg
      121 cgctcgcgct gggcgcgggt gcgctcggca tgcagttcgc aagcattgcc ggcgcggcgg
      181 tgacgatgct gctgctcttc gtgttcgcgc gccgcggcct cttcgcgcgc gcggcaagcc
      241 acgctgcgcg cgcggaagac ggcaccacgc acgtgctgct gaccggcgtg atgatcgccg
      301 cgttctgcgc cgctgcgctg agcctgctgc tgtcgctcgc gccggacgga cggctgcgca
      361 ccatggtgtt ctggctcctc ggtgatctcg gcggcgcgac cagcctgccg tcggcgacca
      421 tcgcgctcgc gctgctggtg ctcgcgctcg tggtgtccga gaagctggcg gcgccgctga
      481 acctgctgct gcgcggcgac gtgcaggcgt tcacccaggg ggtcgacgtc gagcgcacgc
      541 gacgcgcgct cgtgatcgtc gccgcgctcg ccaccggcgc ggcggtcacg ctcgccggcg
      601 cgatcggctt tgttggcttc gtcgcgccgc atctgatgcg gcgcgtgatc ggcaacgatc
      661 agcgcgtgct gcttcccgct gcggcgctgg ccggcggcgt gctcgtcgtc gttgccgaca
      721 ccgttgcgcg caccgccgcc gcgcccctgc agattcccgt cggcgtgctg accgcgatga
      781 tcggcgtacc gatcttcctg tggctgctgc gcacgcgatg agcacgctgc tcgaatgtcg
      841 ccaggtcgac ctgcgcgccg gtcggcgcga actggtgcgc gcgctgtcgc tgcgcatcga
      901 tgccggcgag cgctgggccg tgctggggcc gaacggcgcc ggcaagtcga cgctgctcgc
      961 tgcgctggcg ggcgtgcgcg cgccgaacgg gggcgaaatc ctgctcgcgg gccgcgcgct
     1021 ccaccactgg agcgtcgagc agatggccga gcagcgcgcg ctcgtcaccg atcgctggca
     1081 cgacccgttt tcggcgagcg tgctcgacac cgtgctgacc gcgcgctact ggctcggcgc
     1141 cgaggacgac gccggcgtgc agatcgcgcg cgggtggctg gccgaattcg attgcgccga
     1201 actgatcgac cacgacgtga ccagcctgtc gcgcggcgag cgccagcgcg tcgccctcgc
     1261 gaccgcactg gcgcaggaca cgccgctggt actgctcgac gagcccattg cgcaccagga
     1321 cccgcgccac caggggctgg tgctcgaccg cctcgcgcgc gccccgggcc gcacgctggt
     1381 cgcgtcactg cacgacatga acgccgccgc tcgctttgcc acgcacgcgc tgctgctgac
     1441 gggacgcggc gcttggtcgg caggtcccgc cgcggacatg ctgagcgcgc cgcggctgtc
     1501 ggagctgttc gagaccgacg tgatcacgat ggcgaccccc gacggggcga gggtgttcgc
     1561 gatcggcggc tgactacttg acgccgaacc gcagcgtcgc cgtagcgatg tagacctcgc
     1621 gcaggaagaa cagcaatccg gcgatcagcg acagcagggc ggcggcgaaa acggtcgcga
     1681 caatgcgcgt caggtccggc gaccacgcgt agtacgcggt gatgaagagc cccccgatca
     1741 cgccgcatac cagcagcgcc gccagcacgc acagcgagac cgcgcggttg acgagccgca
     1801 gccgacgcga caaggttcgc agttcgtcat gcagcgcgtg cgcgcgcctg tcgctcgcgg
     1861 tccccaattc gtcctcgacc gtgcgcgcgc ggtcgatgat gcgcgcgacc cgctgcatca
     1921 tcacgccgag cagcgcgccg atgccggtca gcagaaagac cggggcgatc gcgagctgga
     1981 tcacatgcgc aatgatgtcg agctgatcct tcatcggctc tgtcgcgcgg cgtcgagacg
     2041 ctcgcacaat acggtgattt cgtcgacgat gcgcgggccg tggcgattga tccggtcggc
     2101 atcgagcgtg acgagctgcc cgttccgtac cgcgctcacg ccgtcgaagc ggcgccacag
     2161 gtcgagcgcg tcgctcggcc gcgcgccggg ttcggcggtg acgatcacct gcggatcggc
     2221 gacgacgacg gcctcgatcg agacctgagg tgcgatcgcg gtcaacggct caaagatatt
     2281 gcggccgccg cacaggcgaa tcgcctcgtt gatcacgtgc gtgccggcca gtgtcatcag
     2341 cggctgcgcc cagatctgat agaagacgcg caccggcgcg cgcgtcgagt agcgcgcacg
     2401 cagcgccgcg atgcgcgcgc ggaactccgc cgccacctgc ggcgcggtgc ggctgccggt
     2461 cagcacgccc aggcgctcga gcgaggtggc gatgctttcc agcgatcccg gctcgtcgat
     2521 gaacaccggc acgcccagtc gccgtaccga gccgacgatc gccggcggaa agccgctgcc
     2581 ccagatcacg atcaggtcgg gccgcgcggc ggcaacgcgc tccagatcca cgctgtgcgc
     2641 gcgtgccacg cgcggcagct tccgtgccgc cggcgggtag tcggcatact cggtggtggc
     2701 gacgatgcgg tcgccggcgc cgatcgcgaa cagctgctcg gtgatgtgcg gcgcgagact
     2761 gacgatgcgc tgcgcgggcc gggcgagttc aatgcgcgcc tgactgtcgt cgatcgccgc
     2821 gaccggttgt gcggcgctcg cgtccgcgcc gatcagcagg acgagcagcg cggccagggc
     2881 gcgtatcacg gccgcggcgt acctgtcgcc tgtggcggct cggtcacgaa gccgatcctc
     2941 gtcaggccgg cttcctgcgc cagcgacatc accagcgccg cgcgctcgaa gcgcgccgcc
     3001 ttgtcgactc tgagcagcac ctccggcggc ggagtgcgcg ccgccagttc ggcgagccgc
     3061 gtgcgcagtg cctccccttc gaggggctgc tcgttcaggt agatcgcgcc ggcgccgtcg
     3121 atgctcaggc ggacgggctc ggtgctcggg ctgaccggcg tcgacttcac ttcgggcagg
     3181 tcgagcttca gcgcgtaggt gagcagcggt gcggtgatga tgaagatcac cagcaacacc
     3241 agcatcacgt ccaccagcgg cgtgacattg atctccgcca tcggctgcga tgtcgactgc
     3301 ccgcgctcga aaccgccgaa cgtcatcacg tgcccggctg gaagcgcccg ccgacggtca
     3361 gaaacgcatg caggtcgtgc gcgaagccgt cgagctgcgc cagcagcatg cggttgacgc
     3421 gcgtgaacgc gttgtagccg agcagcgcgg gcaccgcgac cacgaggccg aatgcggtca
     3481 tcaccagcgc ctcgccgacg ggaccggcga ccttgtcgat cgccacgctg ccgctcgagg
     3541 cgatgttcag cagcgcgtga tagatgcccc acacggtgcc gaacaggccc acgaacgggg
     3601 cgctgctgcc gaccgaggca agaaaggtca gcccggattc cagtcgcgcg gccgcctcgt
     3661 tgatgcgctg gcgcagcgcg cgcgtgatga gttccgagcg gtcgatcagc gccgacagcg
     3721 actgcgtgcc cggcggcggt gccgcggcgc cggcggcgga cgttgcgagc ggaacgaaga
     3781 tcgcctcggc gtcggcgggc ttgagcagcg cgatcgcctc gtcgatcgag cgcgcggccc
     3841 agaactgctc cagcgcgcgc gccgcgcgcc gcgcccgcag ccaggcccat gccttccaca
     3901 ggatcacgca ccagctcgcc accgacatcg ccagcaggat gtacgcggtc aggcggatga
     3961 tggcgtcggc ctgcgaccag aaatgcgaca ggccgagcga cgggttcatc tcaggccgag
     4021 tacgtcgtgc atgtcgaaga ggccgcgcga gcgctgcgcg agaaagcgtg ccgcgcgcag
     4081 ggcgccgtgc gcgtacgtca tgcggctggc ggagcggtgc gtgatctcga tccgctcgcc
     4141 gatgccggcg aagagcaccg tgtgatcccc gacgatgtcg ccgccgcgca ccgaggcgaa
     4201 tccgatcgtg cccggcttgc gctcaccggt caccccttcg cgcgcgtaca cggccacgtc
     4261 cccgagtttc ttgccgagcg ctgtggcgac gacttcgccc atcttcagag ccgtgcccga
     4321 cggcgcatcg accttgtggc gatggtgtgc ctcgatgatc tcgatgtcgt agccggacga
     4381 caggatctgc gccgcgacct cgagcagctt gaaggtcgcg ttgacgccca cgctcatgtt
     4441 gggcgcgaac acgatcgcga tgccttccgc ggcagcgtgg atcgcggcgc ggcccgcgtc
     4501 gtcgaagccg gtggtgccga tcaccatctt cacgccggcg tccgcgcagg cgcgcaggtg
     4561 ctccagcgtg ccctcgggcc gcgtgaaatc gatcagcacg tcggcgccgc gcagcgcggc
     4621 caggtcatcg atgatgcgca cgccggtcgc gcggccgagg aactcggcgc agtcgcggcc
     4681 cagatcgggg ctccccgcac ggtcgagcgc ggccgcgatc gcaagatcgt cggcgccgag
     4741 cgcggcctcg atcagcatgc ggcccatgcg gccgccggcg ccggcgatcg cgagcttgag
     4801 cgtcatttct gcggcaggtt gatctgcgcc gggggctctt tcggcggcgc gtgcggcagc
     4861 gccttcgggt tgcggccgag gatcaggttg tccgccatcg tctcgggggg catctcgtcg
     4921 gcggtgaacg tgtcgagccg gttgtccttg aagagcaccg tcaggcgccg ccgctgctcc
     4981 tcggtgccct tgccgcgctt caggtgatag atgtagtccc agcggtcctg gtggaacatg
     5041 ctcgccacca acggcgtgcc gagcaggaaa cgaacctggt cgcgcgtcat gcccgggcgc
     5101 agctgctcga ccatctcctt cgtgacgaca ttgccctgct gcacgtcggg gcggtagggc
     5161 tggatgaacg tcggcgtcca gctgcgcatc gattcgcagc cggccagcag cgcggtggcg
     5221 ctgatcagca agacggtgac ggcggtcttg agcgtgtcct tcacggcatc ccttcgaagt
     5281 cgggcgctat gataccgacg caccagcaac cagcgagtcg aagatgcagc agcagcgcag
     5341 cggcacggcg agctcggccg aactgaagag catgggcctg aaagccacgc tgccgcggat
     5401 gaagatcctc gagatcttcc agcgcagttc ggccacgcag ggcgtgcgcc aactgagcgc
     5461 ggaggacgtc tacaaggcgc tgctggccga gaacctcgac gtggggctgg cgaccgtgta
     5521 tcgcgtgctg acgcagttcg agcaggctgg gctggtgaag cgcagtcatt tcgaggccgg
     5581 ccgcgcggtg ttcgaactcg atgaaggcaa gcaccacgat cacctggtgt gcgtcacctg
     5641 cgggcgcgtc gaggaattcg tcgacgccga gatcgagaag cgtcagcagc agatcgccca
     5701 gaccaaggga ttcaagctgc aggagcactc gctcgcgctg tacggcgagt gcacccgact
     5761 caactgtccg catcggcccg gctgagccgg tccccgttca ggccgccagc atctcgcgcg
     5821 cgtgcttgcg cgtggtgtcg gtgatctgca gcccgcccag catgcgcgcc agttcctcca
     5881 cgcgcgtgcg ccgatcgagc aatgcgatgc gcgacaccgg gcggccgtcg tcggcttcga
     5941 ccttgctgac cacgcagtgc tggtcgccgc gcgcggcgac ttgcggcagg tgtgtgacgc
     6001 acaggacctg acgctcgcgc ccgagctgct gcagcagccg tccgacggtt tcggccaccg
     6061 caccgccgat gccggcgtcg acctcgtcga agatcagtgt cgccaccggt gtggcggtcg
     6121 cggcgatcac cgagatcgcg aggctgatgc gcgccagctc gccgccggag gcgaccttgg
     6181 ccaacggctt gggtgcgacg cccgcgtggc cggcgaccag gaattcgacg cgctccaatc
     6241 cctgcgcggc cggttcgccc gcggtcaatg cgatgtcgaa gcggccaccg ggcattgcga
     6301 ggtcctgcat cgcgcgcgtg acttcgcgcg ccatgttggc ggccgccttg gcgcgctgct
     6361 tcgacaacgc acgcgcgagc ttcagatacg cctgctcggc gtccgtctcg cgcttcttca
     6421 gcccgtcgag atcggaggcg gctgccagcg cgttgagctt ctcctgcgac tgcgccagca
     6481 gcgccgcgag atcagccggc gcgcaccgga acttgcgcgc cgccgcgtgc agtgcggtca
     6541 cgcgcgcgtc gagctcggcc aaccgcgcgt cgtcgacctc ggtcttcgcc aggtagtgat
     6601 tcagctcccg cgccgcatcg tcgacctgca cccgcgcagc gtcgagcgtg gcagcgatcg
     6661 gcgcgagccg cgcgtcgaag ccggcaagcg gcgccagccg cgaaagaacg gcgtcgatgc
     6721 gctcgagcgc ggcgccgtcg ccttcgctca gcgcatcgac cgcgccgcgc gcaccctcga
     6781 gcaggcttgc cgcgtgcgac agccgcatgt gttcggcttc gatcgtctcc cactcgcccg
     6841 cctgcggcgc cagcgcggcg agttcgtcga cgacccagcg cagccgctcc tgctcgagcg
     6901 ctgcggcgcc ggccatctgc tcggagcgct cgcgcgcctc gcgttcgcgc cgccacgcgg
     6961 cgtaggccgt ggccaccgcg cgcgcagctg cggtcagtcc gccgtgctcg tcgagcagtg
     7021 ccagctgcgc cgccggccgc agcagcgact ggtgcgcgtg ctggccgtgc acgtcgacca
     7081 gcagctcgcc cagatcgcgc agctgcgcct gcgtgacggc cgtaccgttg atgaatcctt
     7141 tgctgcggcc ggccgcatcg accgtgcggc gcaccagaac cgtgtcctct tccgccgcca
     7201 gttcgttcgc cgcaagccac gccgccgcgg cgccgtcgac gcggaactcg gcgttgatgt
     7261 ccgcgcgtgt cgcgccttcg cgcacgacgc cggcatcggc gcgctcgccg agcgccagca
     7321 gcagcgcatc gatcaggatc gacttgccgg cgccggtctc gccggtcagc accgtgaagc
     7381 cgggcgcgaa ctccaggtcg agcacatcga cgatcacgaa gttgcggatc gacaatccca
     7441 gcagcatcga tgcgtgatca attgcggtgc gcgtcggccg gcatgatgtt ccagtgcagc
     7501 ttctggcgca gcgtggcgaa gtagttgtag ccgaccgggt gcagcagcgt gacgacgttg
     7561 cggccgcggc gcgcgcacac gatgtcgccg gaggcgagcg aggtgaacgc ctgcatgtcg
     7621 aaatgcgcgc ccgcgtcgcg tacgtcggtg acctcgatct cgaccgccac cgagtccggc
     7681 agcgcgatcg ggcggttcga gagcgtttgc ggcgccaccg gcaccagcac gaagccggcc
     7741 agcgacgggt gcaggatcgg gccgctggcg gacagcgcat aggcggtcga gccggtcggc
     7801 gtcgcaagga tcacgccgtc ggcacgctgg ttgtacatcg tcacgccatc aacgcgcacc
     7861 gtgaactcga ccatgccgcc agtgacgccg cgcgacacca cgacgtcgtt cagcgcgcgc
     7921 gcgcggaaca cgaccgcgcc cgcgcgccgc acttcggcct cgagcagcgc acgacggtcg
     7981 gtctcgtact cccccgccag catcgccgcc agcacttccg acagctgcgc caccgcgata
     8041 tcggtgatga agccgagccg gccgagattg atgccgatca gcggcacgtt gaacggtgcg
     8101 agttctcgcg caatgccgag catcgtgccg tcgccgccga gcacgaccgc gacatccgcg
     8161 gtcgcgccga tctgttccgg cgtgtagccg cggtacggat cgaggctcgc cgcatcgcgc
     8221 gtggccgcat cgaacatcac ggtctgcccg gccagctcga cgatgcgggc gacatcgcgc
     8281 agcgtcgaca ccagcgccgg acgggccgcg tccgcgcgcg gcttgccgaa gatcgcgaca
     8341 tgcttgaagg gcgatgccat gggaccaatg tagcaagaac cgtaaatgct gcggagttct
     8401 cgctaatatc cgccttcatg ctggacgacc gcgctcgcct gctgctgaag acgctgatcg
     8461 agcgttacat cgccgaaggt cagcccgtcg gctcgcgctc gctgtcgaag atctccggcc
     8521 tggagctgtc gccggcgacg atccgcaacg tgatggcgga tctggaggag atgggcttcg
     8581 tgtccagccc gcacacgtcg gccgggcgcg tgccgacccc gcgcggttat cggctgttcg
     8641 tggacacgtt gctcaccgtg cagccgatgg agcagcagca ggcggcggcg atcgagggcc
     8701 ggctgcgggt cgccgaaccg cagcgcgcga tcctgacggc ggcgcaactg ctgtcgaacc
     8761 tgtcgcactt cgccggcgtg gtgatgactc cgcggcgcgc gtcgagtttc cgccaggtcg
     8821 aattcgtgcg gctgggcgag cggcgcgtgc tgctgatcat cgtcacgccc gaaggcgacg
     8881 tgcagaaccg catcctgctc accgatcacg cctactcgcc ggcgcagctg gtcgaggcgg
     8941 ccaacattct gaatgagcat tttgccgggc tgagcttcga tgaagcgcgc atgcggctgc
     9001 acggcgatct cgcgcggctg agagaggaca tcgcgcaact gatggaagcc gcggtgcagg
     9061 ccggcagcca ggccgccgag gattccgaac cggtcgtcgt ctcgggcgag cgcaacctgc
     9121 tcggcgtcgc ggacctggcg tcgaacctgg atcacctgcg caagctgttc gatctgttcg
     9181 agcacaagac gcggctgatg cagttgctcg acgtgtcggc gcgcgcgcac ggcgtgcaga
     9241 tctacatcgg cggcgagtcc gcgctggtgc cgctcgacga gatgagcatg gtcgtggcgc
     9301 cgtacgaagt cgacggccag gtcgtcggca cgctcggtgt gatcggaccg acacgcatgg
     9361 cctacgagcg cgtgattccg atcgtcgaca tcaccgcgcg cctgctctcg agcgcgctgt
     9421 cgcgcgacta gcggcgcggc gacgatgcgc tttctgcggg agccagcgta cgcgcacgcc
     9481 gccgccgccc gcactgcggt gctgctggtc aacctcggca cgcccgacgc cccggaaccg
     9541 cgcgcggtgc ggcgctatct gagggagttc ctgtccgatc cgcgcgtggt cgagattccg
     9601 cgcgcgctgt ggctgccgat ccttcacggg atcatcctga acgtgcggcc gcgcaagtcg
     9661 gcagccaagt atgcgtcgat ctggacgccg gcgggctcgc cgctgcgcgt ttactcggag
     9721 cagcagtcgg tgctgctgcg cggctacctc ggccatcgcg ggctcgatgt cgacgtggcg
     9781 ctggcgatgc gctatggcca gccttcgatc ggcgcggtgc tgggcgggct gcgcgagaag
     9841 cgcgtcgagc ggctgctggt gctgccgctg tatccgcagt attcggcgac gaccacggcg
     9901 accgtgttcg atgcggtgca cgcggtgctg gcgcgcacgc gcaacgtccc ggaggtgcgc
     9961 tgggtcaggc atttccacga cgagccgcgc tacgtcgagg cgctgaagac gaatgttctc
    10021 gcgtactgga agaagcaggg ccgcgcgcag gacgcgggcg gcaagctgct gatgagcttt
    10081 cacggcgtgc cgcgccgttc gctcgacctc ggcgaccctt atcactgcga gtgccagaag
    10141 accgcgcgtc tgctggccga ggcgctgcat ttgtcgaagg acgaatacgc ggtcacgttc
    10201 cagtcgcgct tcggccgcgc ggagtggctg cgcccctaca cggcggcgac cctgcacgaa
    10261 cttggagccc agcgcgcccg ccgcgtagac gtgatctgcc cgggcttcgt ggcggattgc
    10321 ctggagacgc tggaagagat cgcgatcgag ggcaggcagg agttcatgtc ggccggcggc
    10381 agcgaatacc actacatccc gtgcctgaac gatgcacccg cgttcatcga ggcgctcgcc
    10441 gacgtggtcg cagcgaacgt gcagggctgg ccggtcgatc gcgcgcagcg cggggcgagg
    10501 gagcagcagg cgcaggcggg agcagcgcgt gcgcggctgc tgggcgcggc gcggtgagcg
    10561 ctgcggcgcg ctccagcgcg actgaacact aggtccgcag tgcggaggcc gccggcgtcg
    10621 cagcttcgcc gtcgggcacg gacgtgacgc tcggcgcagc gggcgctgcc cgggcggtcg
    10681 ccgccacggc aggcgcgagc acggtcgaat tgacgagcag gatcgcggcg attgcagccg
    10741 cggccatcag cacggccggt aactgcgaac tgccgcgctc gccggattcg aattcgaacg
    10801 tgtcgtcgtc gcgcatgact gtctcacacc gatgaacgaa ttgtgcgcag cgcccgcaga
    10861 ggcggatatc ccgcgcgctc gcgccttcaa gcgagtccga ggtcgcggtt tgccgcgatc
    10921 ccgttcgggc taccgcctga ccgaggctgc gcggttcatg cgctgccacg ctccggcgtg
    10981 aactgcggcg tcggggcgag cggggcgccg tcggcggcgc ggaggtcgtc gccctcgacg
    11041 cggacgcgat cacccgcttt cacgccgggg ttaatcgcgc gcgtcagcgt gcgcagcgag
    11101 ccgtcctcca ttcgcaccgt gatctcgtag cggaccgtcg cgcgcgcctg cttctcgatc
    11161 tgatggccgg cgaccgcgcc gccgatcgcg cccaggatgc ggcttgcgtc cttgccgccg
    11221 ccgcgaccga actggttgcc gatcacgccg ccgagcacgc cgcccgcgat cacgccgata
    11281 ccggtgcctt cacccggggc tttcacttca cgcaccgacg tcacggtgcc gcaactggtg
    11341 cagaccggtg cttgcgccgt gaccgacggc actgccggca tctgtgtcag tacgcccggt
    11401 ggcagtggcg ctggcgccgc cgcggccagc tgcaccggcg gcttgggcgt agccggcgct
    11461 gccgttgcgt gcgcgcggct cgccggcagc gcgcgggggg cgggtgctgc cggcgttgcc
    11521 gcagccggag cggacgcgcc ttccgtaggt aatgcggcgg gaaccgccgc cggcgccggc
    11581 gtgtgctgcg ctgatacctg attcgtcacc agcacaacgg cgccgaggac gctgaccgcc
    11641 gtgacggcga ccgccgcggt cgcaatcagc ggatgaagct tgttcatggg agtgccggtt
    11701 ggcatcgaat cctccgagtt gcggcgcgca tcgagcgccg tgcccgcaac aacgggtggt
    11761 gcatgtgcac aggcgacgcc ggaggtgttg cgtgtgtaac cgaatgtgcg cccggggccg
    11821 ctatttgaca cggaattcgc ccgcctgccc gacatagcgt tgggcgttcg ccagccagcg
    11881 ccgcgcggcc gcgtcgtggt cgagatacgc gagccagaac gcggtcgtgg tcgcgcggac
    11941 tacacgcttg tgccgcgcgt ccagcgcggc atcgacggcc gcgccgcgcc aacgttcgcc
    12001 gccgttgaag agcatgtggt cggcgccgac gagattcagc aggtacttgt cgccggcggg
    12061 catgccttcg aatggcagca gccgctgcgc cggtggcacg ccgagcccca tgccgacttc
    12121 tccgtcgcgt gtaccggtga cgctgaagaa cggccgcacg acaggcgcga agcgctgcgc
    12181 gtcggacctc gaacgcgccg acggactgaa ggcgatgaac gcgcgcggcc gcgcatcggc
    12241 cactgcctgc gcgcgggcgc gagcgccgaa gtcctcgccg gcgatcgcct gcgtggtgat
    12301 agcgccgaag gaatgtccgg acaggccgat ccgagcggga tcgatacgcg ccgccaccgg
    12361 gtcgccgacc tggcggcggc gctcgagttc atcgagcaca aactgaacgt cccggacgcg
    12421 gtcgatgaac tgctgggcgc cggcagcggc gcgcagcgcc cgcgccggct tctccgcgcc
    12481 gcgccacacc gactcgtcgc tgcccggatg ctgcacatgg atcacgacga acccatgact
    12541 cgcccactgc tcgccccagt cgcg
//
LOCUS       DBNHBY010000526         2155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_305583, whole genome shotgun sequence.
ACCESSION   DBNHBY010000526 DBNHBY010000000
VERSION     DBNHBY010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_305583"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /gene="sucC"
                     /locus_tag="AC16HB_12855"
     CDS             complement(<1..1133)
                     /gene="sucC"
                     /locus_tag="AC16HB_12855"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR5607722.1"
                     /translation="MKIHEYQGKEILRRYGVAVPRGFPCFSVDEAVAAAKKLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSLDEVRANASAILGMQLVTHQTGPHGQKVRRLLIEEGA
                     DIRKELYVGMVVDRATQRVALMASSEGGMDIEEVAARTPEKIHKVFIDPATGLKDTEA
                     DDIAVKIGVPAASVPAARGVLQGLYKAFWECDASLAEINPLILTGDGRVVALDAKMNF
                     DSNGLFRHPDIVEMRDLDEEDPAEVEASKYDLSYIQLDGDIGCLVNGAGLAMATMDVI
                     KLYGGEPANFLDVGGGATADKVTEAFKIMLRSPKLAAILVNIFGGIMRCDVIAEGVVT
                     ASRAVGLKVPLVVRMKGTNEELGKKILADSGLPIIAADTMAEAA"
     gene            complement(1146..1724)
                     /locus_tag="AC16HB_12860"
     CDS             complement(1146..1724)
                     /locus_tag="AC16HB_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HIR5607723.1"
                     /translation="MPDAPQRRHVHTRTIRVDVYARDDGLWDLEAELIDSKSKDFTLA
                     TGVRKVGEPVHQMTVSVTIDRQLNIVDASATSTWTPYPGYCETIASAYRRLVGLNLIK
                     EFRRHVRERLGGIQGCTHLTELASVLPSAAVQAFANEVYHARDVAHPEHYTDDRKPFQ
                     IDRCHALRSDGAAVAKFYPRWHLAGASKPDSN"
     gene            complement(1808..>2155)
                     /locus_tag="AC16HB_12865"
     CDS             complement(1808..>2155)
                     /locus_tag="AC16HB_12865"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR5607724.1"
                     /translation="AVLDQLQAKGMLSDQRFAAALVRTRGARFGAARIGQELRQRGVE
                     PALIRRSMGALKATELERARDVWRRKFGTLPRDETERARQVRFLTARGFSAEVVLRVI
                     KGAPDDRGSSGDS"
BASE COUNT          332 a          756 c          686 g          381 t
ORIGIN      
        1 gccgcctccg ccatggtgtc ggccgcgatg atcggcaggc ccgagtcggc gaggatcttc
       61 ttgccgagtt cctcgttcgt tcccttcatg cgcaccacca gcggcacctt cagaccgacg
      121 gcacgcgacg cggtgaccac accctccgcg atcacgtcgc agcgcatgat gccgccgaat
      181 atgttgacca ggatcgctgc cagcttcgga ctgcgcagca tgatcttgaa tgcctcggtc
      241 accttgtccg cggtcgcgcc gccgccgacg tcgaggaagt tggccggctc gccgccatag
      301 agcttgatca cgtccatcgt cgccatcgcc aggccggcgc cattgaccag gcagccgatg
      361 tcgccgtcga gctggatgta ggacaggtcg tacttcgatg cctcgacctc ggccggatcc
      421 tcctcgtcga ggtctcgcat ctcgacgatg tccgggtggc ggaagagccc gttggagtcg
      481 aagttcatct tcgcatcgag tgcaaccacg cgcccgtcgc cggtcaggat cagcgggttg
      541 atctccgcca gcgatgcgtc gcactcccag aatgccttgt acagtccctg cagcacgcca
      601 cgcgcggcgg gaacgctcgc ggccggcaca ccgatcttga ccgcgatgtc gtcagcctcg
      661 gtgtccttca gtcccgtggc cggatcgatg aagaccttgt ggatcttctc cggcgtgcgc
      721 gcggcgactt cctcgatgtc catgccgcct tcactggacg ccatcagcgc gacacgctgg
      781 gtggcgcggt cgacgaccat gccaacgtac agctccttgc ggatgtcggc gccctcctcg
      841 atcagcagcc ttctcacctt ctggccgtgc ggcccggtct ggtgcgtgac gagctgcatg
      901 ccgaggatcg cggaggcgtt cgcgcgcacc tcgtcgagcg agcgcgcgag cttgacgccg
      961 ccgcccttgc cacgtccgcc ggcgtggatc tgcgccttga ccacccacac cggcccaccg
     1021 agcttctttg ccgccgcgac ggcctcgtcg accgagaagc atggaaagcc gcgcggcact
     1081 gcgaccccgt agcgacggag gatttccttg ccctggtatt cgtggatctt catgcgcgac
     1141 tcgcttcagt tgctgtctgg cttcgatgca ccggcgagat gccagcgcgg atagaacttc
     1201 gcaaccgctg cgccgtccga ccgcagcgcg tggcagcggt cgatctggaa cggcttgcga
     1261 tcgtcggtgt agtgctccgg atgcgcgaca tcgcgcgcgt ggtacacctc gttggcgaac
     1321 gcctgcaccg ccgcgctcgg cagcacgctg gcaagctccg tcaggtgcgt gcagccttgt
     1381 atgccgccga gacgctcacg cacatggcgt cggaattcct tgatgaggtt cagaccgacc
     1441 agccgccggt atgctgaggc gatcgtctcg cagtagcccg gatacggcgt ccaggtggac
     1501 gtcgcgctcg cgtcgacgat gttcagctgc ctgtcgatcg tgacgctcac cgtcatctgg
     1561 tgcacaggct cgccgacctt gcgcacgccg gtggcgagcg tgaagtcctt cgacttcgaa
     1621 tcgatcagct ccgcctcgag gtcccacagc ccgtcgtcgc gcgcatagac gtcgacgcgg
     1681 atggtgcgcg tgtggacatg gcggcgttgg ggcgcgtcgg gcatgcgcaa tcggaacaac
     1741 gaaaaaaaac cgggcagcgc gggtcgcccg gtgaccggat gttagcaggc gaacgaaccc
     1801 gctgccctca ggaatcgccg ctggatccgc ggtcgtccgg cgctcccttg atcacgcgca
     1861 agaccacctc cgccgaaaag ccgcgcgcgg tcagaaagcg gacctggcgt gcacgctcag
     1921 tctcatcccg cggcagcgtg ccgaacttcc tgcgccacac gtcgcgtgcg cgctcgagtt
     1981 cggttgcctt cagcgcgccc atcgatcgcc ggatcagcgc cggctcgacg ccgcgctggc
     2041 gcaactcctg tccgatgcgg gccgcgccga accgcgcgcc gcgggtgcgc accagcgctg
     2101 cggcgaagcg ctggtcggac agcatgccct tggcctgcaa ctgatcgagc accgc
//
LOCUS       DBNHBY010000527        10584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_981958, whole genome shotgun sequence.
ACCESSION   DBNHBY010000527 DBNHBY010000000
VERSION     DBNHBY010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_981958"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="metX"
                     /locus_tag="AC16HB_12870"
     CDS             complement(<1..191)
                     /gene="metX"
                     /locus_tag="AC16HB_12870"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520843.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HIR5607725.1"
                     /translation="MLKSGSVGVVAPKSLRFAAPLALASGASIGDYMLLYETYGELNA
                     ARSNAVLICHALNASHHVAG"
     gene            complement(381..2183)
                     /gene="ptsP"
                     /locus_tag="AC16HB_12875"
     CDS             complement(381..2183)
                     /gene="ptsP"
                     /locus_tag="AC16HB_12875"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR5607726.1"
                     /translation="MARELVGIGIVSGSAPGVAIGRAHLLGASELEVPQYPIERKDVL
                     HEVARLNNAFSIVRAELDELRGHVSPDAPGEVVAFLDLHRMILDDSNLCDAPRDLVRS
                     KLINAEWALSMQLEELCRQFEAINDEYLRERSQDVRQVVDRVMKALAHGKPQTAMLVP
                     KMAEGERLILIARDMAPADVLHLRDRADLDVAGFVTELGGPTSHTAILARSLSLPAVL
                     GVNGAREAVKEGDLVVIDAEAGRLHIVPEAGALGEFRQRLRAQTEARAQLRRLKGKLS
                     RTRDGVPLDLMANIELPEDARDALEVGADGIGLFRTEFLFLNRDRLPDEDEQFEAYAK
                     VARVMKGKPVVIRTLDIGADKVLNEAARLSLGIVQGAPTVESNPALGLRAIRYCLAYP
                     ELFLTQLRAILRASAVGTVRILVPMLAHVHEVEQALAFVARAREQLKERKQKFDGRVM
                     VGGMIEVPAAALSMPAFVKRMKFFSLGTNDLIQYTLAIDRADSSVAHLYDHLHPAVLH
                     LIARTIRAGMRARTPVSVCGEMAGVVELAELLLGMGLRQYSMHPSQLLTVKERLMTLS
                     TKTAAKLAARVLRADDPMDVRRALERSLARLQAT"
     gene            complement(2192..2461)
                     /locus_tag="AC16HB_12880"
     CDS             complement(2192..2461)
                     /locus_tag="AC16HB_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR5607727.1"
                     /translation="MITREFIINNKLGLHARPSAQLTKAASQFKSEVWIAKAGRRVNA
                     KSIMGVMMLAAGQGSTVTVDADGPDEAAAVDAIGQLITSNFGEHE"
     gene            complement(2430..2894)
                     /locus_tag="AC16HB_12885"
     CDS             complement(2430..2894)
                     /locus_tag="AC16HB_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5607728.1"
                     /translation="MIGVLIIAHAPLASALAQCASHVYACDPTRAESKLRVLDVPAGA
                     DVVSLVAQARALLAEVDAGAGVLVLTDALGATPGNVAAQLSDPPRVAVVAGVNLPMLL
                     RALCYRDGPLADLAEKAIAGGMQGVMQVVATPVQNQNLRPTGDDHARVHHQQ"
     gene            complement(2929..3897)
                     /gene="gshB"
                     /locus_tag="AC16HB_12890"
                     /pseudo
     CDS             complement(2929..3897)
                     /gene="gshB"
                     /locus_tag="AC16HB_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
     gene            complement(3920..5266)
                     /gene="gshA"
                     /locus_tag="AC16HB_12895"
     CDS             complement(3920..5266)
                     /gene="gshA"
                     /locus_tag="AC16HB_12895"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR5607729.1"
                     /translation="MSDPTVPNLTAAVSGPLLELERRILDSMPAIERWFRLQWQDHTP
                     PFYGSVDLRNAAFKLAPVDMNLFPGGFNNLSNEMLPCAVQAAQAAIEHLCPDARNLLL
                     IPERHTRNVYYLTNVARLVRILRSTGLEVRIGSLSAEVAQPTTIALPDSDSLTVEPLL
                     RTDGRVGVAGFDPCAVLLNNDLSAGIPDILRDLDGQVLVPPLHAGWAVRRKSHHFAAY
                     DEVADDFARLLSIDPWLINPYFARCNQVDFHAREGEECLAESVDLVLGRVRAKYKEHG
                     VDAAPFVIVKADAGTYGMGIMTVRDASEVRDLNRKQRNKMAVVKEGLAVRDVIIQEGV
                     PTVEKVEDAVAEPVVYMIDRYVVGGFYRVNKERGIDENLNAPGMHFVPLADAQPFAGS
                     CNLPDCKAPPGGSHQNRFYSYGVVARLALLAASIELDRTANLLPPRAEAAEPALAG"
     gene            5503..6453
                     /locus_tag="AC16HB_12900"
     CDS             5503..6453
                     /locus_tag="AC16HB_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5607730.1"
                     /translation="MSKRRQPRLSLDLLRGFRSAARLLSFTRAARELNVTQSAISREV
                     KTLEDQLGQPLFRRINRALQLTHAGQTLYRATDDALVLIDTVTERIVGSGRTLGVTTT
                     GPLASMWLVPRLPRFTRRHPDIDLRIAASQEALSLDRENLDVAIRYVPPEGGVPSGEL
                     FAPYQIFPVCSPRLARDRVHPLRTPADLARHVRLDFETILYGRPWYDWERWFDTVKLP
                     PVKPAGVQRFWHYDQVVRAAIDGNGVAIGKRPALNEHLRDGTLVAPFGPEWVATLNGY
                     YLEVASSADRQVVDPFIAWLNEELQADEAQIRPRQRKARR"
     gene            complement(6487..8010)
                     /locus_tag="AC16HB_12905"
     CDS             complement(6487..8010)
                     /locus_tag="AC16HB_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5607731.1"
                     /translation="MKKLIAMLCAAAGFALAALPAVTAAQTAPAAEVKKDEAKAEAAP
                     AADAKKDDAKPAEKKEEAKPAPTPNKGDTAWMLTSTALVLLMSVPALALFYGGLVRTK
                     NMLSVLMQVFVTFSLISVLWCVYGYSLAFTEGNAFFGGFDRLFLAGTFDAMKGEFSMA
                     ATFSKATPMYELVFVAFQATFAAITCCLILGSTVDRIKFSAVLIFIVIWFTFSYLPIA
                     HMVWFWAGPDAYTSADVVDKVNATAGKIWQWGALDFAGGTVVHINAGVAGLVGAYLLG
                     KRIGYGRESMAPHSLTMTMIGASLLWFGWFGFNAGSALEANGSAALAFINTFLATACA
                     VLAWTFAEWMFKGKPSMLGAASGAVAGLVAITPAAGNVGILGAFVIGIIAGLICFWGV
                     TGLKRMLNADDALDVFGVHAVGGITGALLTGVFNSQSLGGPGMVTDWVTGKVGSNPIA
                     EQVLIQAKAVGLTVIWTAVVAFIAFKIADLIVGLRVTEEEEREGLDTTYHGESAYHY"
     gene            complement(8027..8365)
                     /locus_tag="AC16HB_12910"
     CDS             complement(8027..8365)
                     /locus_tag="AC16HB_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5607732.1"
                     /translation="MKLITAIIKPFKLDEVREALSAVGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEAAVPEEIVERALEAIENSARTGKIGDGKIFVYDLEQVVRI
                     RTGETGKEAL"
     gene            complement(8379..9146)
                     /locus_tag="AC16HB_12915"
     CDS             complement(8379..9146)
                     /locus_tag="AC16HB_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR5607733.1"
                     /translation="MKKSLASAFAAAAFVCPLTAFAQSAPAKPEPEYTFTGNAGLFSD
                     YRFRGYTQTAYKPAFQGGFDLAHKSGFYIGNWNSNVEQSLYTGASLEMDFYGGYKGTA
                     GDFGYDVGYYYYAYPGSGRAGQPKIDNGEIYVGGSYGPVALKYYYSTTRFFSLGEGTN
                     VDTKGSQYLDLSANYDLGDGWGINGHIGWQKVKNFKQLGAKDDSYTDYKLGVTKDLSG
                     WVLGASLVATSKKEFFTTGAGEDAGKTRLVASVTKTF"
     gene            9359..9595
                     /locus_tag="AC16HB_12920"
     CDS             9359..9595
                     /locus_tag="AC16HB_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR5607734.1"
                     /translation="MEKTTFLDELQGKLEALLANTPAADLQRNLKALLSQQFARLDLV
                     TREEFDVQAKVLARTREKLEALEARLAALENKPR"
     gene            9674..>10584
                     /gene="amrB"
                     /locus_tag="AC16HB_12925"
     CDS             9674..>10584
                     /gene="amrB"
                     /locus_tag="AC16HB_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR5607735.1"
                     /translation="MIIRPAAVAGSFYPAAAAALREEVRAYLGSTPARSGAAPKALIA
                     PHAGYIYSGPIAASAYALLEGARERIERVVLLGPAHRVPVRGLALPGADAFETPLGAV
                     PIDAEGARTLAALPQVEVHARAHAHEHSLEVHLPFLQQTLGDFLLLPLVVGDASTEEV
                     AEVLQAVWGGPETLIVVSSDLSHYLSYTDARRTDAGTARSIVALDATLDHLQACGATP
                     VNGLLTVARRRGLRIEQLDLRNSGDTAGDRARVVGYGAWAMYEPPGAADATSRSDASR
                     RDTLLALARGAIARRLGIGNPPRTEAD"
BASE COUNT         1706 a         3706 c         3466 g         1706 t
ORIGIN      
        1 ccggcgacgt gatgcgatgc gttcagcgcg tggcagatga gcacggcgtt gctgcgcgcg
       61 gcgttgagtt cgccgtaggt ctcgtagagc agcatgtagt cgccgatcga agcaccgctg
      121 gcgagcgcga gcggtgctgc gaagcgcagc gacttcggtg ccaccacccc gaccgatccg
      181 gacttcagca tgcccgccgc aaaaaaagaa aaaacccgac gcgcgctggc ttgtcgggtt
      241 ccgtgcgcgc ccaaccgagg acgcggatct ctttagcgga agttgccggc agcttcgtgc
      301 cggcgcgccc gcaagcggat caacaaatca tcgcgaggcg cgcaatttag cagagaagtg
      361 gcaaaccgca gcgctgcggc tcaggtcgcc tgcagccgcg ccagcgagcg ctccagcgcg
      421 cgtcgcacgt ccatcgggtc gtcggcacgc agcacgcgcg cggcgagctt ggccgcggtc
      481 ttggtcgaca gcgtcatcag ccgctccttg accgtcagca gctgcgacgg gtgcatcgag
      541 tactggcgca atcccatgcc gagcagcagc tcggcgagct cgaccacgcc cgccatctcg
      601 ccgcacacag acaccggcgt acgcgcgcgc atgccggcgc ggatcgtgcg cgcgatcagg
      661 tgcagcaccg cggggtgcag atggtcgtac aggtgcgcca cgctcgaatc ggcgcggtcg
      721 atcgccagcg tgtactggat caggtcgttg gtgccgagcg agaagaactt catccgcttg
      781 acgaacgccg gcatcgacag cgccgccgcc ggcacctcga tcatgccgcc gaccatcacg
      841 cggccgtcga acttctgctt gcgttccttc agttgctcgc gcgcgcgcgc cacgaacgcc
      901 agtgcctgct cgacctcgtg cacgtgcgcg agcatcggta ccaggatgcg caccgtgccg
      961 accgccgagg cgcgcaggat cgcgcgcagc tgcgtgagga aaagctccgg atacgcgagg
     1021 cagtagcgaa tcgcgcgcag tccgagcgcc gggttcgact cgacggtcgg cgcgccctgc
     1081 acgatgccga gcgacaggcg cgccgcctcg ttgagcacct tgtcggcgcc gatgtcgagc
     1141 gtgcggatca cgaccggctt gcccttcatc acgcgcgcga ccttcgcgta cgcctcgaac
     1201 tgctcgtcct cgtcgggcag gcggtcgcgg ttcaagaaca ggaactcggt gcggaacagg
     1261 ccgatgccgt cggcgccgac ctcgagcgcg tcgcgcgcgt cctccggcag ctcgatattg
     1321 gccatcagat cgagcggcac cccgtcccgc gtgcgcgaca gcttgccctt cagccgccgc
     1381 agctgagcgc gcgcctcggt ctgcgcgcgc agccgctgcc ggaactcccc cagcgcgccc
     1441 gcctcgggca cgatgtgcag gcgccccgcc tcggcgtcga tcacgacgag gtcgccttcc
     1501 ttgaccgcct cgcgcgcgcc gttcacgccg agcacggcgg gcaggctgag gctgcgcgcc
     1561 aggatcgccg tgtgcgaggt cggtccgccg agttcggtga cgaaaccggc cacgtcgagg
     1621 tcggcgcgat ccctcaggtg cagaacgtcg gccggcgcca tgtcgcgcgc aatcaggatc
     1681 agccgctcgc cctcggccat cttcggcacc aacatcgcgg tctgcggctt gccgtgcgcg
     1741 agcgccttca tcacgcgatc gacgacctgg cgcacgtcct gcgagcgctc tcgcaggtac
     1801 tcgtcattga tcgcctcgaa ctgccggcac agctcctcga gctgcatcga cagcgcccat
     1861 tcggcgttga tcagcttgct gcgcacgagg tcgcgcggcg catcgcacag gttcgaatcg
     1921 tcgaggatca tccggtgcag atcgaggaat gccacgacct cgcccggcgc atccggcgat
     1981 acatgcccgc gcagctcgtc gagctcggcg cgcacgatcg agaacgcgtt gttcagccgc
     2041 gccacctcgt gcagcacgtc cttgcgctcg atcggatact gcgggacctc gagctccgag
     2101 gcgccgagga ggtgcgcgcg gccgatcgcg acccccgggg ccgaccccga gacgatgccg
     2161 atgccgacca gttcgcgcgc caccccgtcc cctactcgtg ctcgccgaaa ttcgacgtga
     2221 tcagctgccc gatcgcatcg accgcggccg cctcgtccgg tccgtcggcg tcgaccgtca
     2281 ccgtgctgcc ctggccggcg gcgagcatca tcacgcccat gatcgacttg gcgttcacgc
     2341 gccggccggc cttggcaatc cagacttcgc tcttgaactg cgacgccgcc ttggtgagct
     2401 gcgccgaggg ccgcgcgtgc agtccgagct tattgttgat gatgaactcg cgcgtgatca
     2461 tcgcccgtcg gccggaggtt ctgattctgt accggtgtcg ccacgacctg catcacgccc
     2521 tgcatgccgc ccgcgatcgc tttttccgcg agatcggcca gcgggccgtc ccggtagcac
     2581 agcgcgcgca gcagcatcgg caggttcacg ccggcgacca ccgccacacg cggtgggtcc
     2641 gacaactgcg ccgcgacatt gcccggcgtg gcgccgagcg catcggtcaa caccagcaca
     2701 cccgcgcccg cgtcgacctc ggcaagcaat gcgcgtgcct gggctaccag gctcacgaca
     2761 tcagcgccgg ccggcacgtc gagcacgcgc agcttgcttt cggcccgcgt cggatcgcac
     2821 gcgtacacgt gcgacgcgca ctgcgcgagc gcgctcgcca gcggcgcgtg ggcgatgatc
     2881 aacacaccga tcatgagcgc gccggccgcc gcgggcctgg tttccacgtc atcggctcaa
     2941 cgccctttcc agcgcgtcga tgaacatgcc ggcgacattg aacccggtct gctgcgtgat
     3001 ctcgacgaag caggtcgggc tggtgacgtt gacttcggtc aggtagtcgc cgatcacgtc
     3061 cagccccacc agcagcaggc cgcgcttgac cagcaccggg ccgagcgcgg cggcaatctc
     3121 gcggtcgccg tcggtaagcg gctgcgcacg gccgatgcca ccggcggcca gattgccgcg
     3181 cgtttcgccc ggcttcggga cgcgcgccag cgcatgcggc acggcttcgc ctccaatcag
     3241 cagcacgcgc ttgtcgccct ccttgatctg cgggatgtag cgctgcgcca tcaccgtgcg
     3301 ctcgccgagc cgcgtgatcg tctacaggat cacgttcagg ttcgcgtcgt ccggccgcag
     3361 ccggaacacg ctcatgccgc ccatgccgtc gagcggcttg accaccacgt cgccgtgctc
     3421 gcgcaggaat gcacggagtg catccacatc gcgcgtgacc agcgtcggcg gcgcgaactc
     3481 ggcgaattcg gcgatcgcga acttctcgtt gtgatcgcgc agcgcccgcg ggtcgttgaa
     3541 gacccgcgtg ccctgccgct gggccgcctc gagcaggaag gtcgcgtaaa ggtattcgag
     3601 gtcgaacggc ggatccttgc gcatcagcac cgcgtcgaac gacgcgaggt cctgcagccc
     3661 cgactcgcgc tcgacgtacc atggggcgtg cttgtcgtcc gacgcggccg ccagcgacag
     3721 cagcgtcgcc tcggcctgca cgcggctgcc cgctccgtcg ctgctccatc gcagttgcgc
     3781 cggcatgcac gcccatactt cgtggccgcg cgcggccgcc tcgcgcatca tcgccaccga
     3841 tgaatccttg tacgccttca gcgcttcgag cggatcgacg acgaacagca gcgacagtcc
     3901 ttgcgacatc gaacctcgcc tagccggcca gcgccggctc ggccgcctcg gcgcgcggtg
     3961 gcagcaggtt cgcggtgcga tccagttcga tcgacgccgc cagcagcgcc agccgcgcca
     4021 ccacgccata gctgtagaac cggttctggt ggctgccgcc cggcggcgcc ttgcagtcgg
     4081 gcaggttgca cgagccggcg aatggctgtg cgtccgccag cggcacgaag tgcattccgg
     4141 gcgcgttcag gttttcgtcg atgccgcgct ccttgttgac gcgatagaag ccgccgacca
     4201 cgtagcggtc gatcatgtag acgaccggct cggcgactgc atcctcgacc ttctccaccg
     4261 tcggcacgcc ctcctggatg atcacgtcgc gcaccgccag cccttccttg acgaccgcca
     4321 tcttgttgcg ctgcttgcgg ttcagatccc gcacctcgct cgcgtcgcgc acggtcatga
     4381 tccccatgcc gtaggtgccg gcgtcggcct tgacgatcac gaagggcgcc gcatcgacgc
     4441 cgtgctcctt gtacttggcg cgcacgcgcc caagcacgag gtcgacgctc tcggccaggc
     4501 actcctcacc ttcgcgcgca tggaagtcca cctggttgca gcgcgcgaaa tacgggttga
     4561 tcagccaggg gtcgatcgac agcaggcgtg caaagtcgtc ggcgacctcg tcgtatgcgg
     4621 cgaagtggtg cgacttgcgc cgcaccgccc agcctgcgtg caacggcggc accagcacct
     4681 gtccatccag atcgcgcagg atgtccggta taccggccga caggtcgttg ttcagcagga
     4741 cggcgcacgg atcgaagccg gcgacgccga cgcgaccgtc tgtgcgcagc aagggctcga
     4801 ccgtcaggct gtcgctgtcg ggcagtgcga tcgtggtcgg ctgcgcgacc tcggccgaca
     4861 gcgacccgat gcgaacttcc agcccggtcg aacgcaggat gcggacgagc cgcgcgacgt
     4921 tcgtcaggta gtagacgttg cgcgtatgcc gctccgggat cagcagcagg ttgcgcgcgt
     4981 ccgggcacaa gtgctcgatc gcggcctggg cggcctgcac cgcgcacggc agcatctcgt
     5041 tggacaggtt gttgaatccg cccgggaaga ggttcatgtc caccggtgcc agcttgaaag
     5101 cggcgttgcg cagatcgacc gagccataga acggcggggt gtggtcctgc cactgcagcc
     5161 ggaaccagcg ctcgatcgcc ggcatggaat cgaggatgcg gcgctcgagt tcgagcagcg
     5221 ggccgctgac ggcggcggtc aggttcggga cggtgggatc ggacacggca ggctccctgc
     5281 acgcacgtcg gacgggcatt ctagccagag atatgggggt cgccgcgccg caaaaaagaa
     5341 gggcgcccct atttggggcg cccgaaagcc gcctttggtg cggccgcgct gcttcggaac
     5401 gcggccaggc gagctaggga gacacgtctt ccctcatgca aggcgcatgc cggccgcaga
     5461 tctgacttat cgtggcgcca tgcgctttac cgtgtccgaa gaatgagcaa gcgccgccag
     5521 ccgcggttgt cgctcgatct gctgcgcggc ttccgctcgg ccgcgcggct gctgagtttc
     5581 acgcgcgccg cgcgcgaact gaacgtcacc caatcggcga tcagccgcga ggtgaagacg
     5641 ctcgaagatc agctcggcca gccgcttttt cgccgcatca accgcgcgct gcagctcacg
     5701 catgcagggc agacgctgta ccgcgcgacc gacgatgcct tggtcctgat cgataccgtg
     5761 accgagcgca tcgtcggcag cggccgcacg ctcggcgtca cgacgacggg cccgcttgcc
     5821 tcgatgtggc tggtgccgcg cctgccgcgt ttcacgcgcc gccatccgga catcgatctg
     5881 cgcatcgcgg caagtcaaga ggcgctgagc ctggatcgcg agaacctcga cgtcgcgatc
     5941 cgctatgtcc cgccggaagg cggcgtgccg agcggcgagc tcttcgcgcc gtatcagatc
     6001 ttcccggtgt gttcgccgcg cctggcgcgt gatcgtgtgc acccgctgcg cacgcccgcg
     6061 gatctagcgc ggcacgtgcg gctggacttc gagacgattc tgtacgggcg gccctggtac
     6121 gactgggagc gctggttcga cacggtcaag ctgccgccgg tcaagccggc cggcgtgcag
     6181 cggttctggc actacgacca ggtggtgcgc gccgccatcg acggcaacgg ggttgccatc
     6241 ggcaagcgcc cggcactgaa cgagcacctg cgcgacggaa cgctggtcgc gccgttcggg
     6301 cccgagtggg tcgcgacgct gaacggctac tacctggaag tcgcgtccag cgccgatcgc
     6361 caggtggtcg atccgttcat cgcctggctg aacgaggaac tgcaggccga cgaggcacag
     6421 attcgtccgc gccaacgcaa agcccggcgt tagccgggcc ttgttccaac aagcgaagcg
     6481 cgcccgtcag tagtgatagg cggactcgcc gtggtacgtg gtgtcgagtc cctcgcgctc
     6541 ctcctcctcg gtcactcgca acccgacgat caggtccgcg atcttgaagg cgatgaacgc
     6601 gacaaccgcg gtccagatga ccgtcagtcc gaccgccttg gcctggatca gcacctgctc
     6661 ggcgatcgga ttcgagccga ccttgccggt gacccagtcg gtgaccattc ccggcccgcc
     6721 gaggctctgc gagttgaaca cgccggtcag cagcgcgccg gtgatgccgc cgaccgcgtg
     6781 cacgccgaac acgtcgagcg cgtcgtccgc gttgagcatg cgcttcaggc ccgtgacgcc
     6841 ccagaagcag atcagtccgg cgatgatccc gatcacgaac gcgccgagga tgccgacgtt
     6901 gccggccgcc ggcgtgatcg ccaccagccc tgcgacggcg cccgacgcgg cgccgagcat
     6961 cgagggcttg cccttgaaca tccattcggc gaacgtccac gccagaaccg cgcacgcagt
     7021 ggcgaggaac gtgttgatga atgccagcgc ggcggaacca ttcgcttcca gcgccgagcc
     7081 ggcgttgaaa ccgaaccagc cgaaccacag cagcgacgcg ccgatcatcg tcatcgtcag
     7141 gctgtgcggc gccatcgatt cgcggccata gccgatccgc ttgccgagca ggtatgcgcc
     7201 caccaggccc gcgacgccgg cattgatgtg caccaccgtg ccgccggcga agtccagagc
     7261 accccactgc cagatcttgc ccgcggtcgc gttgaccttg tccacgacgt cggccgacgt
     7321 gtacgcatcg ggaccggccc agaaccacac catgtgcgcg atcggcagat agctgaacgt
     7381 gaaccagatc acgatgaaga tcagcaccgc ggagaacttg atgcggtcga cggtcgaacc
     7441 gagaatcagg cagcaggtaa tcgcggcgaa cgtggcctgg aacgcgacga acacgagctc
     7501 gtacatcggc gtggccttgg aaaaggtcgc cgccatcgag aactcgccct tcatcgcgtc
     7561 gaaggtcccg gcaaggaaca gccggtcgaa gccgccgaag aacgcgttgc cctccgtgaa
     7621 cgcaagactg tagccgtaca cgcaccacag caccgagatc agggagaagg tcacgaacac
     7681 ctgcatcagc acgctgagca tgttcttcgt gcgcaccagc ccgccgtaaa agagggccag
     7741 cgccggcacc gacatcagca gcaccagcgc ggtggaggtc agcatccacg ccgtgtcgcc
     7801 cttgttgggc gtcggcgcgg gtttggcctc ttccttcttc tcggccggct tggcgtcgtc
     7861 tttcttcgcg tccgccgcgg gcgcggcctc cgccttcgcc tcgtccttct tcacttcggc
     7921 ggcaggcgca gtctgcgccg ccgtcaccgc cggcagtgcc gcgagcgcga agcccgccgc
     7981 ggcgcacagc attgcaatga gctttttcat ggttcccctg tcgttgttag agcgcttcct
     8041 tgccggtctc gccggtgcgg atgcggacga cctgttcgag gtcgtagacg aaaatcttgc
     8101 cgtcgccgat cttgccggtg cgcgccgagt tctcgatcgc ctcgagcgcg cgctcgacga
     8161 tctcctcggg tacggccgcc tccagcttga ccttgggcag gaagtcgacc acgtattccg
     8221 cgcctcgata cagctcggtg tggcctttct gccgtccgaa gcccttgacc tcggtgaccg
     8281 tgatgccctg cacgccgacg gcgctcaggg cctcgcgcac ctcgtcaagc ttgaacggct
     8341 tgatgattgc ggtgatgagt ttcatgagat gcctccgctc agaacgtctt tgtcaccgac
     8401 gcgacgaggc gcgtcttgcc ggcgtcctcg ccggctccgg tggtgaagaa ctccttcttg
     8461 ctcgtggcga cgagcgacgc gccgagcacc catccggaaa gatccttggt cacgccgagc
     8521 ttgtagtcgg tgtagctgtc gtccttcgcg ccaagctgct tgaagttctt gaccttctgc
     8581 caaccgatgt ggccgttgat gccccatccg tcgccgaggt cgtagttggc agacaggtcg
     8641 agatactgcg aacccttggt gtcgacgttc gtgccctcac cgagcgagaa gaacctcgtc
     8701 gtcgagtagt agtacttcag cgccaccggg ccgtacgagc caccgacgta gatttcaccg
     8761 ttgtcgatct tcggctggcc ggcgcgaccg ctgccgggat aggcgtagta gtagtagccg
     8821 acgtcgtagc cgaagtcgcc ggccgtgccc ttgtagccgc cgtagaagtc catctcgagg
     8881 ctggcgcctg tgtacagcga ctgctcgacg ttcgagttcc agttgccgat gtagaagccc
     8941 gatttgtgcg ccaagtcgaa gccgccctgg aacgccggct tgtatgcggt ctgcgtgtag
     9001 ccgcggaagc gatagtcgct gaacagcccg gcgttgcccg tgaacgtgta ctcgggctcc
     9061 ggcttggccg gagcggactg cgcgaacgcc gtcagcgggc aaacgaacgc ggcggcagcg
     9121 aaagcggatg caaggctctt cttcatctat tcctcggttc aaaggacaag ggggaaactc
     9181 caccccatcc tttgcaaccc gcgtgccagc ggccgaactt gccggaaatc gcccggcctc
     9241 tgcgcaggcg tgcgccaaac gcgtgcatgc gctgtccgaa gcggtgcgcc agggcgcaaa
     9301 ctgcacccga tccgggcagg gcttagactg gccgcgctgc gctccgaccg acaccgcgat
     9361 ggaaaagacc acattcctgg atgaactgca gggcaagctg gaggcactgc tggcgaacac
     9421 gccggcagcg gacctgcagc gcaatctcaa ggcgctgctg tcgcagcagt tcgcgcggct
     9481 cgatctggtc acgcgcgagg aattcgatgt ccaggcgaaa gtgctcgcgc gcacccgcga
     9541 gaagctcgag gcgctggaag cgcggctggc cgcgctcgaa aacaagccgc gctgaacatc
     9601 gcggacggct tgattccagc ccggttcggt ccaagatagt gcttgacgtg atggaccgcg
     9661 caacgcgcat tcgatgatca tccgaccggc cgccgtggcc ggcagcttct atcccgccgc
     9721 agccgccgcg ctgcgcgagg aggtgcgtgc gtatctcggc agcacgccgg cacggagcgg
     9781 cgccgcaccc aaggcgctga tcgcgccgca cgcgggctac atctactccg ggccgatcgc
     9841 cgccagcgcg tacgcgctgc tggagggcgc gcgtgagcgc atcgagcgcg tcgtgctgct
     9901 cggccccgcg catcgcgtgc cggtgcgcgg gctggcgttg cccggtgccg acgcattcga
     9961 gaccccgctc ggcgccgttc cgatcgatgc cgaaggcgca cgcacgctgg ccgcgctgcc
    10021 gcaggtcgaa gtgcatgccc gcgcccatgc ccatgaacat tcgctcgagg tgcacctgcc
    10081 gttcctgcag cagacgctcg gcgatttctt gctgctgccc ctggttgtcg gcgatgcaag
    10141 caccgaggag gtcgccgagg tgctgcaggc ggtctggggc ggccccgaga cgctgatcgt
    10201 cgtcagctcg gatctgtcgc actacctgtc gtacaccgac gcgcgacgca ccgatgcagg
    10261 caccgcgcgg tcgatcgtcg cgctcgacgc gacgctcgat catctgcagg cgtgcggcgc
    10321 cacgccggtc aacggcctgc tcaccgttgc acgccggcgc ggactgcgta tcgagcagct
    10381 cgacctgcgc aactccggcg acaccgcggg cgaccgcgcg cgcgtggtcg gctacggcgc
    10441 ctgggccatg tacgagccgc cgggcgccgc ggacgcaacc tcgcgcagcg acgcgtcgag
    10501 gcgcgacacg ctgctcgcac tggcacgcgg ggccatcgcg cggcggctcg gcatcggcaa
    10561 cccgccgcgc actgaagcgg acta
//
LOCUS       DBNHBY010000528         2716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_845926, whole genome shotgun sequence.
ACCESSION   DBNHBY010000528 DBNHBY010000000
VERSION     DBNHBY010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_845926"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..797
                     /locus_tag="AC16HB_12930"
     CDS             381..797
                     /locus_tag="AC16HB_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607736.1"
                     /translation="MLPPSLAPLSVFLVEDNLVIRENLAATLEEMAPVRVIGSAADET
                     SAVAWLAAFEIPCDLVIVDLFLQHGSGFGVLQAARHLRPSCAPIVLSNYASPDVRRRC
                     LSLGADRVFDKSRDIEALIDYCRQLAGSRPAGACPT"
     gene            complement(809..1441)
                     /locus_tag="AC16HB_12935"
     CDS             complement(809..1441)
                     /locus_tag="AC16HB_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607737.1"
                     /translation="MIRVGIVDDHAIVRAGLRQFLGEHADLQVVGEAANGREALDLVR
                     SGNIDVLVLDLSMPEHDGVGALTAIRTRAPQLPILILSAFAESHYATALLKQGASGYL
                     NKECDPEEIVNAIRTVHSGRRYITPAVAELLAAALDGDADQPPHEQLSQRELQVFLRL
                     ARGESVGDIARGMALSVKTVSTYRTRVLEKMKLGSNSDLTYYALKNGLIQ"
     gene            1486..>2716
                     /locus_tag="AC16HB_12940"
     CDS             1486..>2716
                     /locus_tag="AC16HB_12940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF017077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5607738.1"
                     /translation="MRIPSSLRSGRAALLLFAVVVAIVAAANEGAYWRSRRSMETLDA
                     SAAARADLRALLVDMKDAEWAQRSYLLSGRDEQLQACRASLAAVGQALQRLDAHYREA
                     AALARLGRLAEAVQARQTDIEATLALYAAGERERAIGQTLVDRGGTQTVTIHQQAAEL
                     DAAEATLQAAARARVDRTLLVSRVGIDALAALSLLVLFLYVRQARAIGEMRARQAREI
                     QAERDQLEREVGRRTAQLTELARHLQSAREDERRRLARELHDELGALLTAAKLDAARI
                     RSRLGAREPEALERLDHLNQALNDGIALKRRIIEDLRPSSLDNLGLVAALEILAREFE
                     QTAKVVVRCRLESVRLAPDAELTVYRLVQEAFTNIAKYARARVVEVSVAARDGMAEVE
                     VVDDGVGFDPDAHTQSAH"
BASE COUNT          411 a          912 c          971 g          422 t
ORIGIN      
        1 atggggcatc ggcggcgcgt tggtcgcggt gccgctgctg ctggcactgc gcgcagcgtg
       61 caagcgaacg cgccggctca agttgctttg cgtctacctg gagggcggag tgacgccctc
      121 tccaagcctg cgttcgctgt tgcgaatcag gcgagtcccg gcattgagcc gacgctgagc
      181 aagcaaagtg ccgcggtgcg acggcaggca cgccgacgag gtcaccagcg ggcagctgac
      241 ggacagcggt cgcgtgccga ccttcgacga ctgccgggtc gaggccgagc gacgtgcgca
      301 agcggcgtag gaaccgtcct acaccggcgc acgccgccgc ctgatggaca tcgtgcgggc
      361 gcgcgtcatg atgcgcgagc atgttgccgc cctcgctcgc tccgctttcg gtgttccttg
      421 tcgaagacaa tctcgtgatc cgcgagaacc tggcggcgac gctggaagag atggcgccgg
      481 tgcgcgtgat cgggtcggcg gccgacgaga cttcggcagt ggcatggctg gccgcgttcg
      541 agatcccgtg cgacctggtg atcgtcgatc tgtttctgca gcacggatcg ggattcggcg
      601 tgctgcaggc ggcgcgtcat ctgcgcccgt cgtgcgcgcc catcgtgctg agcaactatg
      661 cctcgcccga tgtccgccgc cgctgcctgt cactcggcgc ggaccgcgtg ttcgacaagt
      721 cgcgcgacat cgaggcgctg atcgactatt gcaggcagct cgcgggcagc cggccggcgg
      781 gcgcatgccc gacatgacgc gccgaccgct actggatcag gccgttcttc agcgcgtaat
      841 aggtcaggtc gctgttcgac ccgagcttca tcttttccag cacgcgcgtg cggtaggtgc
      901 tgacggtctt gaccgaaagc gccatgccgc gcgcgatgtc gccgaccgac tcgccacgcg
      961 cgagccgcag gaacacctgc agctcgcgct gcgacaactg ctcgtgtggc ggttggtcgg
     1021 cgtcgccatc gagtgcagcg gcgagcagtt ccgcgacggc gggcgtgatg taccgccggc
     1081 ccgaatgtac ggtgcggatc gcgttgacga tctcttccgg atcgcactcc ttgttcagat
     1141 agccgctggc accctgcttc agcaacgcgg tcgcgtagtg cgactcggca aacgcgctca
     1201 ggatcaggat aggcagctgc ggggcgcgcg tccggatcgc tgtcagcgca ccgaccccgt
     1261 cgtgctccgg catcgacagg tccagcacca ggacgtcgat gttgccgctg cgtaccaggt
     1321 ccagcgcctc gcggccgttg gcggcttcgc cgacgacctg caggtcggcg tgctcgccca
     1381 ggaactggcg caggccggcg cgcacgatcg cgtgatcgtc cacaatgccg acacggatca
     1441 tcggcagcac tccgagcaga tgacaggacg cgcagtctag tcccgatgag aattccatcg
     1501 tccttgcgca gtgggcgtgc ggcgctgctg ctgtttgcgg tcgtcgtggc catcgtggcg
     1561 gcggccaacg agggcgccta ctggcgatcg cgccggtcga tggaaacgct cgatgcaagc
     1621 gccgccgcgc gtgccgacct gcgcgccctg ctggtcgata tgaaggatgc cgaatgggcg
     1681 caacgcagct atctgctgtc cgggcgcgac gagcagctgc aggcctgtcg cgcatcgctc
     1741 gccgcggtcg ggcaggcgct gcagcgcctg gacgcgcatt accgggaggc cgctgcgttg
     1801 gcccgcctcg gccggctcgc ggaagcggta caggcgcggc agaccgacat cgaggcgacc
     1861 cttgcgctct atgcggcggg agagcgcgag cgcgcgatcg ggcagacgct ggtcgaccgc
     1921 ggcggcacgc agaccgtgac gattcaccag caggccgccg agctggatgc cgccgaagcg
     1981 acgctgcagg ccgccgcgcg cgcacgcgtc gatcgcacgc tgctcgtcag ccgcgtgggc
     2041 atcgacgcgc tggcggcgct gagcctgctc gtgctgttcc tctacgtgcg tcaggcgcgc
     2101 gcgatcggcg agatgcgggc gcgccaggcg cgcgaaatcc aggccgagcg cgatcaactc
     2161 gagcgcgagg tcgggcgccg taccgcgcag ctcactgaac tggcgcggca cctgcagtcg
     2221 gcgcgcgagg acgagcgccg tcgactcgcg cgcgaactgc atgacgaact cggtgcgctg
     2281 ctgacggcgg ccaagctcga cgcggcacgc atccgctcgc ggctcggcgc gcgcgagccc
     2341 gaggcgctgg agcggctgga ccatctgaac caggcgctga acgacggcat tgcgctgaag
     2401 cggcggatca tcgaggacct gcggccgtca tcgctcgaca acctgggcct ggtcgctgcg
     2461 ctggagatcc tggcgcgcga gttcgagcag acggcgaagg tcgtcgtgcg atgccgcctc
     2521 gaatcggtgc gcctggcacc ggacgcggag ctgaccgttt accggctcgt gcaggaggca
     2581 ttcaccaata tcgccaagta cgcgcgcgca cgcgtcgtcg aagtcagcgt cgccgcacgc
     2641 gacggcatgg ccgaggtcga ggtcgtcgac gacggcgtcg gcttcgatcc cgacgcgcac
     2701 acgcagtcgg cgcacg
//
LOCUS       DBNHBY010000529         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1253734, whole genome shotgun sequence.
ACCESSION   DBNHBY010000529 DBNHBY010000000
VERSION     DBNHBY010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1253734"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..827
                     /locus_tag="AC16HB_12945"
     CDS             54..827
                     /locus_tag="AC16HB_12945"
                     /inference="COORDINATES: protein motif:HMM:NF021472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HIR5607739.1"
                     /translation="MDTMLDHRLAQVAPGPRVRRVVPSRTIGWLRAGWNDLSQAARPS
                     IGYGVVVAGAGALLIALAWGASHLVPALSGGFLLVAPCVAVVFYELSRQIERGDRIDG
                     RAALLAWRRNADSLALWGLTLALTLILWERLAAIIFALLYGGQVADLHSLAIEVLTGR
                     RLGLLLAYVAVGGALALAVFALGWVTAPLLLDRDIDVASAALTSLRCCLRNPGTALVW
                     ATLLALITTIGFATLMIGMIVVFPWLGHASWHAYRELVE"
     gene            complement(1030..>2114)
                     /gene="glcF"
                     /locus_tag="AC16HB_12950"
     CDS             complement(1030..>2114)
                     /gene="glcF"
                     /locus_tag="AC16HB_12950"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR5607740.1"
                     /translation="GPRGRIYLIKQVLEGVAPTAQTQLHLDRCLTCRSCETTCPSGVQ
                     YGRLLDIGRKVVDDRVGRPAGAALLRAALKEGLTSSLFAPAMKVGQLFRPLLPATLKK
                     KVPAKQAAGAWPARTHARRMLLLAGCVQPAMLPNINAATARVLDALGVEFVVPKAAGC
                     CGAIRYHLNDHDGGLADARRNIDAWWPQIESGCEAIVMNASGCGAQVRDYGHLLRLDT
                     AYAAKARRVSELTRDVAELLPGLLSGRKPTHLDAARVVFHSPCTLQHAQQIRGTVEAL
                     LASVGAEVLPFAESHLCCGSAGTYSIFQPELSTRLRDRKLGHLQQSRPDAILSANIGC
                     IAHLASGTDTPVLHWIEWLDARLRAG"
BASE COUNT          279 a          725 c          757 g          353 t
ORIGIN      
        1 ccgacggcgg cgtgccgaag caacactgga atgaccgcag cgaaggaggc gccatggata
       61 cgatgctgga ccatcgcctt gcgcaggtgg cgcccggacc gcgcgtgcgg cgcgtcgtgc
      121 cgtcgcggac gatcggctgg ctgcgggcgg gttggaacga cctctcgcag gcggcgcgac
      181 cgagtatcgg ctacggcgtg gtggtggccg gcgcgggcgc gctgctgatc gcgctcgcgt
      241 ggggcgcttc gcacctggtg cccgcgctga gcggcgggtt cctgctggtc gcaccgtgcg
      301 tggcagtggt gttctacgaa ctgtcgcggc agatcgagcg cggcgatcgc atcgacggtc
      361 gcgcagcgct gctcgcctgg cggcgcaatg ccgattcgct cgcgctctgg ggcctgacgc
      421 tcgcgctgac gctgatcctg tgggagcggc tggcggcgat catcttcgcg ctgctgtatg
      481 gcgggcaggt cgccgatctg cattcgctgg cgatcgaggt gctgaccggc cggcggcttg
      541 gactgctgct cgcctacgtc gcggtcggcg gtgcactggc gctcgcggtc ttcgcgctgg
      601 gatgggtcac cgcgccgctg ctgctcgatc gcgacatcga tgtggcgagc gccgcgctga
      661 ccagcctgcg ctgttgcctg cgcaacccgg gcacggcgct ggtgtgggcg actttgctcg
      721 cgctcatcac cacgatcggc ttcgcgacgc tgatgatcgg gatgatcgtc gtgtttccgt
      781 ggctcggcca cgcgagctgg catgcatatc gcgaactggt cgagtgagaa gccgtgaatg
      841 aatctactgc gctcctcgat cttgcgcctc cggtgctcac cgtacgacgc tgtacgcgtc
      901 ccgtccacgg ccgcgcatgc aggcgcggcc ggctgcggcg gcgcgaagcg tgcgcttcgc
      961 gaaaccccgc cttcgccctc ggcgcaagct cggggcgctc gctacgattt cttcacgact
     1021 tctgacggct cagccggcgc gcagccgcgc gtcgagccac tcgatccagt gcagcaccgg
     1081 ggtgtcggtg ccgctcgcga ggtgtgcaat gcagccgatg ttggccgaca ggatcgcgtc
     1141 cggtcgcgac tgctgcagat ggccgagctt gcggtcgcgc agccgggtgg acagttccgg
     1201 ctggaagatc gaataggtgc ccgcggagcc gcagcacagg tgcgattccg cgaacggcag
     1261 cacctcggcg ccgaccgagg cgagcagcgc ctcgacggtg ccgcggatct gctgcgcgtg
     1321 ctgcaatgtg cacggcgaat ggaacaccac gcgcgccgcg tcgaggtggg tcggcttccg
     1381 ccccgaaagc aatcccggca gcagctccgc cacgtcccgg gtcagttcgc tcacgcgccg
     1441 cgcttttgcc gcgtacgcgg tgtcgagtcg cagcagatgg ccgtagtccc tgacttgcgc
     1501 gccgcagccg ctcgcgttca tcacgatcgc ctcgcagccg gattcgatct gcggccacca
     1561 ggcgtcgatg ttgcggcgtg cgtcggccaa cccgccgtcg tggtcgttca ggtgatagcg
     1621 gatcgcgccg cagcagccgg ccgccttcgg caccacgaac tcgacgccga gcgcgtcgag
     1681 cacgcgcgcc gtcgcggcat tgatgttggg cagcatcgcc ggctgcacgc agcccgcgag
     1741 cagcagcatc ctgcgtgcgt gcgtgcgcgc gggccacgcg cccgcagcct gtttcgccgg
     1801 taccttcttc ttcagcgttg ccggcagcag cggtcggaac agctgtccca ccttcatggc
     1861 cggcgcgaac agcgaactcg tcagcccttc cttcagcgcg gcgcgcagca gcgcagcacc
     1921 ggcggggcgg ccgacgcggt catcgaccac cttgcggccg atgtcgagca ggcgcccgta
     1981 ctggaccccc gacgggcacg tcgtttcgca actgcggcag gtcaggcagc ggtcgaggtg
     2041 aagctgcgtc tgcgcggtcg gcgccacgcc ctcgagcacc tgcttgatca ggtagatgcg
     2101 gccgcgcggg ccat
//
LOCUS       DBNHBY010000530         2170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_711401, whole genome shotgun sequence.
ACCESSION   DBNHBY010000530 DBNHBY010000000
VERSION     DBNHBY010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_711401"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1069)
                     /locus_tag="AC16HB_12955"
     CDS             complement(194..1069)
                     /locus_tag="AC16HB_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5607741.1"
                     /translation="MSEWVDLAALRDWLPEMKSGLRVVAILAVAWVAQRVVERVLRRL
                     RVLLAARAGDRESAKRIETVVRAIRYGLTVVILLVAGMLMLNELGVSIAPILGAAGVV
                     GLAVGFGAQSLIKDYFNGFFLLVEDQIRVGDVVEIAGVGGLVEEITLRRVKLRGYDGA
                     VHYVANGLITTVTNRASGFAYAVMDIGVAYKESIDAAIAVLHDVARGLRADPAFAPRI
                     LEDLEVAGVENLTDSAVVLRCRLKVAPLEQWSVRREFLKRVKSALDVAGLEIPFPQRT
                     VWVRGDAPAGASAPR"
     gene            complement(1066..1845)
                     /gene="otsB"
                     /locus_tag="AC16HB_12960"
     CDS             complement(1066..1845)
                     /gene="otsB"
                     /locus_tag="AC16HB_12960"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR5607742.1"
                     /translation="MKHLFGAEGAAELARLMTAHALYAFDFDGTLAPIEARPEHVRTP
                     ANVMRLLQQLAQVAPVAVISGRRRADLVARLPAEVRYLVGNHGNEGGPIAADAEAMRA
                     VCGGWHAQLGAWIAAEPDAHDVLVEDKTLSLSLHYRLARDRPRAARRLERWIERLAPP
                     PRVIGGKLVFNLLPPGALTKFEALAAIADSERAERVLFVGDDDTDELVFAQAPAHWLT
                     VRVELRRASTARYFVHQQSGVAMLLGHLARRGLASDSEARE"
     gene            complement(1880..>2170)
                     /locus_tag="AC16HB_12965"
     CDS             complement(1880..>2170)
                     /locus_tag="AC16HB_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HIR5607743.1"
                     /translation="ARDDERGVLILSQFAGAARELHEALVVNPYHVEEVAAALHRALV
                     MPVAEVRERMRSLRQLVREFNVYRWAARMLRDAARLRRRERVAERIEVHTIE"
BASE COUNT          331 a          828 c          712 g          299 t
ORIGIN      
        1 ccgcactccc ccctccagtc gcggtcgccc ttcggcggtc ggccggcgtg ccgagcgcgc
       61 acacgcccgg cgcaggggaa gatgctcgac acgatgagct tcagaatctc gcgcttgagc
      121 ggcatgaccc tgccaccccg gcgcagggtg ttcacgcact gccttgttct tccctgaata
      181 cgaggggcga ggcttatcga ggcgccgacg ctcccgccgg cgcgtcgcca cgtacccaga
      241 ccgtacgctg cggaaacggg atctcgaggc ccgccacgtc gagggccgac ttgacgcgct
      301 tgaggaactc gcgcctgacg ctccactgct cgagcggcgc caccttgagc cggcaacgca
      361 gcacgaccgc ggaatcggtc aggttctcca cgcccgcgac ctcgaggtcc tcgagtatgc
      421 gcggcgcgaa tgccgggtcg gcgcgcaacc cgcgcgcgac gtcgtgcagc accgcgatcg
      481 cggcgtcgat gctctccttg tacgcaacgc cgatgtccat caccgcgtat gcgaatccgc
      541 tggcgcgatt ggtgacggtc gtgatcaggc cgttggcgac gtagtgcact gcgccgtcgt
      601 agccgcggag cttgacccgg cgcagcgtga tctcctcgac caggccgccg acgccggcga
      661 tctcgaccac gtcgccgacg cgaatctgat cctcgaccag caggaagaag ccgttgaagt
      721 aatccttgat caggctctgc gcgccgaaac cgaccgcgag cccgaccacg cccgcggcac
      781 cgaggatggg cgcgatcgaa acgccgagct cgttcagcat cagcatgccg gccacgagaa
      841 ggatcaccac ggtcaagccg tagcggatcg cgcgcaccac cgtctcgatg cgcttggccg
      901 attcgcggtc gccggcgcgc gcggcgagaa gcacacgcag ccggcgcagc acgcgctcga
      961 cgacgcgctg cgcgacccag gccactgcca ggatcgcgac gacgcgcagc cccgatttca
     1021 tctcgggcag ccagtcgcgc agcgccgcca ggtcgaccca ctcgctcatt cgcgcgcctc
     1081 cgagtccgac gccaggccgc gtcgggccag atgcccgagc agcatcgcca cgcccgactg
     1141 ctggtgcacg aagtaacgcg ccgtgctcgc gcgccgcaac tcgacccgca ccgtcagcca
     1201 gtgcgccggc gcttgcgcga atacgagttc gtcggtgtcg tcatcgccga cgaacagcac
     1261 ccgctccgcg cgctccgagt cggcgatcgc ggccagcgcc tcgaacttgg tcagcgcgcc
     1321 gggcggcagc aggttgaaca cgagcttgcc gccgatcacg cgcggcggcg gggcgagccg
     1381 ttcgatccag cgttcgagcc ggcgcgcggc gcgcgggcgg tcgcgcgcca gccggtagtg
     1441 cagcgacagc gacagcgtct tgtcctcgac cagcacgtcg tgcgcgtccg gctcggccgc
     1501 gatccaggcg ccgagctggg cgtgccagcc gccgcacacc gcgcgcatcg cctccgcgtc
     1561 ggcggcgatc ggtccgcctt cgttgccatg gttgccgacc aggtaccgca cctcggccgg
     1621 caaccgcgcg acgagatccg cgcgccggcg gccggagatc accgccaccg gcgccacctg
     1681 cgccagctgc tgcagcagcc gcatcacgtt tgccggcgtg cggacatgct ccggccgcgc
     1741 ctcgatcggc gccagcgtgc cgtcgaagtc gaacgcgtac agcgcgtgcg cagtcatcag
     1801 ccgcgccagt tcggcggcgc cttcggcgcc gaacagatgc ttcatcggcg ccgcgccctt
     1861 ctctttcgtt gcgcagagat cactcgatcg tatgcacctc gatccgctcc gccacgcgct
     1921 cgcgtcgccg cagccgcgcg gcgtcgcgca gcatgcgcgc cgcccagcgg tagacgttga
     1981 actcgcgcac caactggcgc aggctgcgca tccgctcgcg cacctccgcc accggcatca
     2041 ccagcgcgcg gtgcagcgcc gcggcgacct cctcgacgtg atacggattg acgacgagcg
     2101 cctcgtgcag ttcccgtgcg gcgccggcaa attgggacag gatcagcacg ccgcgctcgt
     2161 cgtcgcgcgc
//
LOCUS       DBNHBY010000531         3759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1321013, whole genome shotgun sequence.
ACCESSION   DBNHBY010000531 DBNHBY010000000
VERSION     DBNHBY010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1321013"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="cutA"
                     /locus_tag="AC16HB_12970"
     CDS             <1..231
                     /gene="cutA"
                     /locus_tag="AC16HB_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR5607744.1"
                     /translation="RLAPVASTYRWEGEIEDAEEIPLLIKCTRERYPAVEQMIRELHP
                     YTVPEIVAVPVVAGFGPYLRWVDDETQPPLVA"
     gene            228..614
                     /locus_tag="AC16HB_12975"
     CDS             228..614
                     /locus_tag="AC16HB_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR5607745.1"
                     /translation="MNDERVRLDKWLWAARFFKTRSLAVREVDLGRVRVDGERVKPAH
                     EVRCGERIEIQLSSQRIEIVVQALSMVRGPAPVARQLYAETAESRERRARLAERYHYG
                     AEPAAGIKGRPSKKEGRALRGLRRSE"
     gene            complement(634..1602)
                     /locus_tag="AC16HB_12980"
     CDS             complement(634..1602)
                     /locus_tag="AC16HB_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR5607746.1"
                     /translation="MTTRVEVANPAGQARVWVPLPLAQDTDYFRTLANRWSGNAEQAE
                     MVIEPTYRAAMAVAQFKPGEPAPVLEVVSRFAVRERSVPFAPGAGMPATRDELALALK
                     PTDLLPTDGIVKKTADEVTKGARTDVAKARAIYDWVVENTVRDPKTRGCGIGDIKEML
                     ETGALGGKCADLNALFVGLCRAAGVPARDVYGIRVVESPRGYKSMGKLGDITKAQHCR
                     AEFYAQGVGWVPVDPADVRKVVLEEKPNLSLNDDVVKVMRTFLFGNWEGNWLAYNYAH
                     DVRLPGSKGAPVGFLMYPQAETADGRLDALDPENFRYKLTSREVAT"
     gene            complement(1849..2286)
                     /locus_tag="AC16HB_12985"
     CDS             complement(1849..2286)
                     /locus_tag="AC16HB_12985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5607747.1"
                     /translation="MRPWPGPPPAPPIELRTLDGRAFALADMRGKVVLVNFWATWCEP
                     CIEELPSMQRLRDLLGPEQAEVLGVNYQEGEPRIRAFLKQVPLDFPIVRDTDGGVART
                     WNVRIFPTTFVVDADQSIRYLVTGSLDWSAPEIERRIRALLPR"
     gene            complement(2406..3545)
                     /locus_tag="AC16HB_12990"
     CDS             complement(2406..3545)
                     /locus_tag="AC16HB_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR5607748.1"
                     /translation="METVHVQPDQALRLEAVPTAPCTNGFYWLDLTHEEFVADPERLR
                     DVVAQLTGARLFDLHLQDAANLQHPSYFDSTSDYDMLIFRKLTPVGDRTVLPLTDLDQ
                     HPQLARRGHRLQEIVTRPVTFFLFDHLLVTVRNAQSRTIEQVRTRLLEFRARHIQKSP
                     LAANEVPMTRLPQRPEELLLRLLNGMVDRYLDLRQPLTDRLERWQRELLDPRRPFNDW
                     GALLDARIEIRRLENLCEEQHDALQEMRDTYLEETPEAQQSDAYLVRIADVIEHIHRV
                     LNHARRLESSIETAVQLHFSATAHRTNQVVRILTVITAVFAPLTLITGIFGMNFEHMP
                     LLKDREGFWLTIFGMVALAAALLLYFAVNRMLSDRPLRVRRWWRV"
     gene            complement(3553..>3759)
                     /locus_tag="AC16HB_12995"
     CDS             complement(3553..>3759)
                     /locus_tag="AC16HB_12995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898350.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR5607749.1"
                     /translation="RVKDEFRAPTFVYQVSGEYAMLMAAARNGWLDEKKVILEALLAF
                     KRAGADGVLTYFALTVARWLKEGR"
BASE COUNT          628 a         1245 c         1247 g          639 t
ORIGIN      
        1 cggctcgcgc cggtcgcgtc gacctaccgc tgggagggcg agatcgagga tgccgaggag
       61 attccgctgc tgatcaagtg cacacgcgaa cgctatccgg cggtcgagca gatgatccgc
      121 gaactacatc catacacggt gccggagatc gtcgcagtcc cggtcgtggc cggattcggc
      181 ccttatctgc gctgggtcga cgacgagacg cagccgccgc tcgtcgcatg aacgacgaac
      241 gcgtccggct cgacaaatgg ctgtgggccg cccgcttctt caagacacgc tcgcttgctg
      301 tgcgcgaggt cgacctcggg cgcgtgcgcg tggatggcga acgcgtgaaa cctgcgcatg
      361 aggtgcgctg cggcgagcgg atcgagatcc agctctcttc gcagcggatc gaaatcgtcg
      421 tgcaagcgct gtcgatggtc cgtggtccgg cgccggttgc gcgccagttg tacgcagaaa
      481 ctgccgagag ccgagagcgc cgcgcacggt tggccgagcg ctatcactac ggtgccgagc
      541 cggccgccgg gatcaaaggg cgccccagca agaaggaagg gcgcgctctg cgcggtctgc
      601 gccgcagtga atgagcgcgc cggtgcgttc ccgctatgtg gcgacctcgc gtgaggtcag
      661 cttgtagcgg aagttctccg ggtcgagcgc gtccagacgc ccatccgcgg tttccgcctg
      721 cggatacatc aggaagccga ccggtgcgcc tttcgagccc ggcaggcgca cgtcgtgcgc
      781 gtagttgtag gcgagccagt tgccttccca gttgccgaac aagaacgtgc gcatcacctt
      841 cacgacgtcg tcgttaagcg acaggttcgg tttctcctcc agcacgacct tgcgcacatc
      901 ggccgggtcg acgggcaccc agcccacgcc ttgggcgtaa aactcggcgc ggcaatgctg
      961 cgccttggtg atgtcgccca gcttgcccat gctcttgtag ccgcgtgggg attcgaccac
     1021 gcggatgccg tacacgtcgc gcgccggaac cccggcggcg cggcacagcc cgacgaacag
     1081 cgcgttcagg tcggcgcact tgccgccgag ggcgcccgtt tccagcatct ccttgatgtc
     1141 gccgatgccg cagccgcgcg tcttggggtc acgcaccgtg ttctcaacca cccagtcgta
     1201 gatcgcgcgt gccttggcca cgtcggtacg cgctcccttg gtgacttcgt cggcggtctt
     1261 cttcacgatg ccgtcggtcg gcagaaggtc ggtcggcttc agtgccagcg cgagttcatc
     1321 ccgcgtggcc ggcatgccgg cgcctggtgc gaacggaacg ctgcgttcgc gcactgcgaa
     1381 ccggctcacg acctccagga ccggcgccgg ttcgcccggc ttgaactgcg ccaccgccat
     1441 cgccgcacga taggtcggct caatcaccat ctcggcctgt tcagcgttgc cgctccagcg
     1501 gttcgcgagc gtgcggaagt aatcggtgtc ctgtgcgagc ggcagcggta cccaaacgcg
     1561 tgcttgcccg gcgggattgg cgacttcgac ccgggtcgtc acctcgaagg agcgccagcc
     1621 gcccggtgcg gcgggcgtgg ccgattgggc gcgggagaga acgggaacgg cggccgcgat
     1681 tccggctgcg ggtacggccc ttagcaatga gcgacgacgc attcaatctc ctggactgaa
     1741 gttcaaagcg gaatgaggcc gagccgcggc ggcggcccgg agcctgcgaa agacttgctc
     1801 gtcctcgagg ggatgaggac cggcgctcga aaatatacgt ccgcttcttt agcgcggcag
     1861 tagcgcacgt atgcggcgct cgatctccgg cgcgctccag tcgagacttc cggtgacaag
     1921 gtagcggatg ctctgatccg catcgaccac aaacgtcgtg gggaaaatgc gcacgttcca
     1981 cgtgcgcgcc acaccgccgt cggtgtcacg cacgatcggg aagtcgagtg gaacctgctt
     2041 caggaacgca cgaatccgcg gctcgccttc ctggtagttg accccgagta cttcggcctg
     2101 ctctgggccg agcaggtcgc gcagccgctg catcgatggc agctcctcga tgcagggctc
     2161 acaccaggtc gcccagaaat tcaccagaac gaccttgccg cgcatgtccg ccagcgcaaa
     2221 cgcgcggccg tccagcgtgc gcagctcgat cggtggcgcg ggcggcgggc caggccaggg
     2281 ccgcaaagtc gcctgggcgc gggcgcgcgc ggcgaatatg accgcgacgc caccggcgct
     2341 ggccaacagc gttcgcctga gtcgaccaaa agtgtccatc gcacctccgt ctagccccgt
     2401 cgcggctaga cccgccacca gcgccgcacg cgcagcggcc ggtcgctcag catgcggtta
     2461 accgcgaagt acagcagcag cgccgccgcc agcgccacca tgccgaagat cgtcaaccag
     2521 aagccctcgc gatccttgag cagcggcatg tgctcgaagt tcatgccgaa aattcccgtg
     2581 atcagcgtca gcggcgcgaa caccgccgtg atgaccgtca gaatgcgcac gacctggttg
     2641 gttcgatgcg cagtggcgga aaagtgcagc tgcaccgcgg tctcgatcga cgattccagc
     2701 cggcgcgcat ggttcagcac gcggtggatg tgttcgatca cgtcggcgat ccgtaccagg
     2761 taggcgtcgc tttgttgcgc ctccggcgtc tcctcgagat aggtgtcgcg catctcctgc
     2821 agtgcgtcgt gctgttcctc gcacaggttt tcgaggcgtc ggatctcgat ccgcgcgtcg
     2881 agcagcgcgc cccaatcgtt gaacggccgg cgcgggtcca gcagttcgcg ctgccagcgt
     2941 tcgagccgat cagtcagcgg ctggcgcaga tcgaggtagc gatcgaccat gccgttcagc
     3001 aggcgcagca gcagttcctc gggccgctgc ggcagtcgcg tcatcggcac ctcgttcgcc
     3061 gccagcgggc tcttctggat gtggcgcgcg cgaaattcga gcaatcgcgt gcgtacctgc
     3121 tcgatcgtgc gtgactgcgc gttgcggacg gtgaccagca gatgatcaaa caggaagaac
     3181 gtcaccgggc gcgtgacgat ttcctgcagc cgatggccac gccgcgccag ttgaggatgt
     3241 tggtcaagat ccgtcagcgg cagcaccgtg cgatcgccga cgggtgtgag cttgcggaat
     3301 atcagcatgt cgtagtccga tgtcgaatcg aagtacgacg gatgctgcag gttggcggcg
     3361 tcctgcaaat gcagatcgaa cagccgcgcg cccgtcagtt gcgcgactac gtcgcgcagt
     3421 cgctcggggt cggcaacgaa ttcctcgtgc gtcaggtcga gccagtagaa cccgttcgtg
     3481 catggcgcag tcggcaccgc ttcgagccgc agcgcctggt ccggctggac atgaaccgtc
     3541 tccacgacgc ggtcagcggc cttccttcag ccagcgcgcc acggtcagcg cgaagtaggt
     3601 cagcacaccg tcggcgccgg cgcgcttgaa cgcgagcagc gcttcaagga tcaccttctt
     3661 ctcgtccagc cacccgttgc gcgcggcggc catcagcatc gcgtactcgc cgctgacctg
     3721 gtagacgaag gtcggcgcgc ggaactcgtc cttcacgcg
//
LOCUS       DBNHBY010000532         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1793395, whole genome shotgun sequence.
ACCESSION   DBNHBY010000532 DBNHBY010000000
VERSION     DBNHBY010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1793395"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="AC16HB_13000"
     CDS             complement(<1..561)
                     /locus_tag="AC16HB_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5607750.1"
                     /translation="MATNKYARVLILGSGPAGYTAAVYAARANLKPMLITGLAQGGQL
                     MTTTDVDNWPADADGVQGPELMERFLKHAQRFDTDVVFDHIHTVSLKQRPFRLVGDSG
                     EYTADALIIATGASAKYLGLASEQAFMGKGVSGCATCDGFFYRNQAVCVVGGGNTAVE
                     EALYLTNIASKVHVIHRRDKFRAEPIL"
     gene            complement(689..1465)
                     /gene="xth"
                     /locus_tag="AC16HB_13005"
     CDS             complement(689..1465)
                     /gene="xth"
                     /locus_tag="AC16HB_13005"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR5607751.1"
                     /translation="MPLTVATWNVNSLKVRLPHLTDWLAAHGTDIVCLQETKFTDDKF
                     PVDELTKAGYASVFAGQKTYNGVAVLVRSGTAAADVQVNLPNFSDEQKRLLAVTVDGL
                     RVVCAYFPNGQAVDSDKYDYKLNWIAALNEWLRGELARHDKLALAGDYNIAPEPRDVH
                     DPVLWDGQVLFSAPERAAFRGLLDLGLKDSFRLFEQPERSYTWWDYRNMAFRRKWGLR
                     IDHILLSPTLAAQCTACTIDVEMRRREQPSDHAPVIATIA"
     gene            complement(1502..2089)
                     /locus_tag="AC16HB_13010"
     CDS             complement(1502..2089)
                     /locus_tag="AC16HB_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR5607752.1"
                     /translation="MIRTIAVVLALGIAGTAGAQYKWVDKDGRTGFGDTPPRDAKSVQ
                     RLDLRGGGGDSADASKEFPYELKRAADRFPVTLYTAASCQPCELARSFLRNRGIPYGE
                     RTVNNEGDAEELKRISGGIRLPVLAVGRQVQVPFDPDVWTSALDAAGYPRGSMLPRSY
                     QPPAPQPLTARAPEPAKAETAKAEPAKGAPPVARQ"
     gene            complement(2101..>3299)
                     /locus_tag="AC16HB_13015"
     CDS             complement(2101..>3299)
                     /locus_tag="AC16HB_13015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR5607753.1"
                     /translation="LVPKMAAAPAEVASFLRDLARRARPHGERDIEELRDFAVRELGI
                     ADLRVWDHAYASEKLREARYAYSEQELKQYFPEPRVLAGLFRVVETLYSVRIRPDQAP
                     VWHPDVKFFRIESPSGELIGQFYMDLYAREHKQGGAWQGDARGRRRTLAGLQTPVSFL
                     TCNFTRPVGGRPALFTHGEVLTLFHEFGHGLHHLLTRVDELGVSGLHGVEWDAVELPS
                     QFMENFCWEWEPLQWLSGHVDTGAPLPRVLYERMIAAKNFQSGMQMLRQIEFGLFDLR
                     VHAELDPDRATPASVRELLQEVRREVAVVHPPEYNRMPWSFTHVFAGGYAAGYYSYKW
                     AEVLSADAYAAFEEAGNALDATMGARFRDEVLAVGGARSAMESFVAFRGRKPTIDALL
                     RHHGLA"
BASE COUNT          480 a         1124 c         1149 g          546 t
ORIGIN      
        1 caggatcggc tcggcgcgga acttgtcgcg gcggtggatc acgtgcacct tggacgcgat
       61 gttcgtcagg tacagcgcct cttcgaccgc ggtgttgccg cccccgacca cgcacaccgc
      121 ctggttgcga tagaagaaac cgtcgcaggt ggcgcagccc gacacgccct tgcccatgaa
      181 cgcctgctcg ctcgccagcc cgaggtactt ggccgaggcg ccggtggcga tgatcagcgc
      241 gtcggcggtg tattcgccgc tgtcgccgac caggcggaac ggccgctgct tgaggctgac
      301 cgtgtggatg tggtcgaaca cgacgtcggt gtcgaagcgc tgcgcatgct tgaggaagcg
      361 ttccatcagc tcgggtccct gcacgccgtc tgcatcggcg ggccagttgt cgacgtcggt
      421 ggtcgtcatc agctgcccgc cctgcgccag ccccgtgatc agcatcggct tcaggttcgc
      481 gcgcgccgcg tatacggcag cggtgtagcc cgccggcccg gagccaagaa tcaggacgcg
      541 ggcgtacttg ttggttgcca tgacttctcc ctgcgtggcg caagggccac ttaccgtcgg
      601 tcgattatag gtggggggta ttacagggat cgcaagcgtg gtcacgctta cgactgcaat
      661 ggtttgcgct ttccgccgcg cggttgtgct acgcgatcgt cgcgatcacc ggcgcatgat
      721 cgctcggctg ctcgcgccga cgcatctcga cgtcgatcgt gcacgcggtg cactgcgcgg
      781 cgagcgtggg cgacagcagg atatggtcga tgcgcaggcc ccatttgcgg cggaacgcca
      841 tgttgcggta gtcccaccac gtgtagctgc gctcgggctg ctcgaacagg cggaagctgt
      901 ccttcagccc caggtccagc agcccgcgga acgcggcgcg ctcgggtgcg gagaacagca
      961 cctggccgtc ccacagcacc gggtcgtgca cgtcgcgcgg ctcgggtgcg atgttgtagt
     1021 cgccggcgag cgcgagcttg tcgtggcgcg cgagttcgcc gcgcagccac tcgttcagcg
     1081 ccgcgatcca attcagcttg tagtcgtact tgtcgctgtc gaccgcctgg ccgttcggaa
     1141 agtacgcgca cacgacgcgc agcccgtcga cggtgacggc gagcaggcgc ttctgctcgt
     1201 cggagaagtt gggcaggttg acctgcacgt cggcggcggc ggtgccgctg cgcaccagca
     1261 ccgccacgcc gttgtaggtc ttctgcccgg caaagacact cgcatagccg gccttcgtca
     1321 gttcgtcgac gggaaacttg tcgtcggtga acttggtttc ctgcaggcac acgatgtcgg
     1381 tgccgtgcgc ggcgagccag tcggtcaggt gcggcaggcg caccttcagc gagttgacgt
     1441 tccaggtggc gacggtgagc ggcacggccg gcgcgcggtc agcggcgcgc gatgtagcgg
     1501 tctactggcg cgcgactggc ggcgcgccct tcgcgggttc cgccttcgcg gtctcggcct
     1561 tcgccggctc cggcgcgcgc gcggtcagcg gctgcggggc agggggctgg tagctgcgcg
     1621 gcagcatcga gccgcgcggg tagccggcgg cgtcgagcgc cgaggtccag acatcgggat
     1681 cgaacggcac ctgcacctgc cgcccgaccg cgagcaccgg caggcggatg ccgccgctga
     1741 tgcgcttcag ttcctcggcg tcaccctcgt tgttcacggt gcgctcgccg tacggaattc
     1801 cgcggttgcg caggaacgaa cgcgccagtt cgcacggctg gcacgacgcg gcggtgtaca
     1861 gcgtgacggg gaagcggtcg gcggcccgct tcagttcgta agggaattcc ttcgacgcgt
     1921 cggcgctgtc gccgccgccg ccgcgcaggt cgagccgctg cacgctcttg gcatcgcgcg
     1981 gcggggtatc gccgaagccg gtgcggccgt ccttgtcgac ccacttgtac tgcgcgccgg
     2041 ccgtgccggc aattcccagc gcaagaacga ctgcgatcgt gcgaatcatg tccgactccg
     2101 tcaggcaagg ccgtggtgcc gcagcagcgc gtcgatggtc ggcttgcgtc cgcggaaggc
     2161 gacgaacgat tccatcgccg accgcgcgcc gcccactgcc agcacctcgt cgcggaagcg
     2221 cgcgcccatc gtcgcatcga gcgcgttgcc cgcttcctcg aacgcggcgt aagcgtccgc
     2281 cgacagcacc tcggcccact tgtagctgta gtagcccgca gcatagccgc cggcaaatac
     2341 gtgcgtgaag ctccacggca tgcggttgta ttcgggcgga tggactaccg cgacctcgcg
     2401 ccgcacttcc tgcagcagct cgcgcacgct ggcgggcgtg gcccggtcgg ggtcgagctc
     2461 ggcgtgcacg cgcaggtcga acaggccgaa ctcgatctgc cgcagcatct gcatgccgct
     2521 ctggaagttc ttcgccgcga tcatcctttc gtacagcacg cgcggcagcg gggcgcccgt
     2581 gtcgacgtgg ccgctcagcc actgcagcgg ctcccactcc cagcagaagt tctccatgaa
     2641 ctggctcggc agttcgaccg cgtcccactc gacgccgtgc aggccggaga cgccgagctc
     2701 gtcgacacgc gtcagcaggt ggtgcaggcc gtggccgaac tcgtggaaca gcgtcagcac
     2761 ctcgccgtgc gtgaagagcg ccggtcggcc tccgaccggc cgcgtgaagt tgcaggtcaa
     2821 aaaggaaacc ggcgtctgca gtcccgcgag cgtgcggcgc cggccgcgtg cgtccccctg
     2881 ccacgcgccg ccctgcttgt gctcgcgcgc gtacagatcc atgtagaact ggccgatcag
     2941 ctcgcccgag ggcgattcga tgcggaagaa cttcacgtcg ggatgccaca cgggggcctg
     3001 gtcgggacgg atccgcaccg agtacagcgt ctcgaccacg cggaacaggc cggcgagcac
     3061 gcgcggctcg gggaagtact gcttcagctc ctgctccgag tacgcatagc gggcctcgcg
     3121 cagcttctcg ctcgcgtagg cgtggtccca gacccgcagg tcggcgatgc cgagctcgcg
     3181 caccgcgaaa tcgcgcagtt cttcgatgtc gcgttcgccg tgcgggcgcg cgcggcgcgc
     3241 gaggtcgcgc aggaacgagg cgacctccgc cggcgcggcg gccatcttcg gcaccagcg
//
LOCUS       DBNHBY010000533         6196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_238395, whole genome shotgun sequence.
ACCESSION   DBNHBY010000533 DBNHBY010000000
VERSION     DBNHBY010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_238395"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1657
                     /gene="leuS"
                     /locus_tag="AC16HB_13020"
     CDS             <1..1657
                     /gene="leuS"
                     /locus_tag="AC16HB_13020"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR5607754.1"
                     /translation="RVPVWVGNYVLMGYGEGAVMGVPAHDERDFAFAQKYGLPIQQVI
                     AIEGETFSLGGWQEWYADKQRGVCVNSGKYDGLGYEAATDAIAADLKAKALGDKQIQY
                     RLRDWGISRQRYWGTPIPIIHCDACGSVPVPEKDLPVLLPEDLVPDGSGNPLDKDARF
                     LACKCPQCGQPAQRETDTMDTFVDSSWYYMRYCSPDAATMVDARNDYWMPMDQYIGGI
                     EHAVLHLLYARFWTKVMRDLGLVRFDEPFTRLFTQGMLLNDCYFREDESGRTRWFYPN
                     EVEVSFDDRGRPTGAIAKEDGQPVMLGGIEKMSKSKNNVVEPRDIIAKFGADTARAFV
                     MFAGPPDMSAAWSDSGAEGVYRFLRRLWTFCYAQRDAIKGAASIDGTALPAKLQDVRR
                     ELHLALKQALFDYQRMQYNTVVSATMKMLNALDDARLGDDAASRALAREGTSLLLRTL
                     YPIAPHITHTLWTELGFAAVHGDLLDAALPAVDEGALRQDEIELVVQVNGKLRGSIRV
                     PAQADKAAIERAAMADANVQKFVGTNPVKRVVVVPNKLVNVVV"
     gene            1657..2151
                     /locus_tag="AC16HB_13025"
     CDS             1657..2151
                     /locus_tag="AC16HB_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR5607755.1"
                     /translation="MRRALLAAIGLAPLAAACGFRLRGARELPFETIYLGFGPNSPLG
                     SELARNIRAGTSTKVVDNAKDAQAVFEILSQSTDREVLSVNAQGRAREFTLRQRLTFR
                     LHDGKGSEYLGPTQVAVQRDVAFDDAQALAFEAETALLYRDMQTDLVQQILRRLAAAK
                     GVRG"
     gene            2148..3182
                     /gene="holA"
                     /locus_tag="AC16HB_13030"
     CDS             2148..3182
                     /gene="holA"
                     /locus_tag="AC16HB_13030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5607756.1"
                     /translation="MILKQEALAGHLERAAHAGKLARIYTIASDEALLSIEAQDAIRA
                     AARRAGFAEREVLHADGRFDWSLLLGATTGLSLFAEKKIVEVRLPSGKPGKSGAEALR
                     EHASNATDATLAIVALPRLDRATRESAWAAALEHAGAWIDIAKVERAELPAWIGQRLA
                     RQQQRAARAALEFLADRVEGNLLAAHQEIGKLALLYPPGEITLEQLTESVLNVARYDV
                     FALPAALYAGDSARAVRMLEGLRAEGEALPLVLWAVSEEIRTLLKAKSAADAGRPPGT
                     LARELRIWGPREKLLPQALARLTAASLSALLARCADVDRLVKGLRVERRDSDPWLELS
                     DIALALATGR"
     gene            3179..4471
                     /locus_tag="AC16HB_13035"
     CDS             3179..4471
                     /locus_tag="AC16HB_13035"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR5607757.1"
                     /translation="MNAPQELRTDLASYMKDVGSRARRASAAMAKAATATKNAALTSL
                     AAAIRARRAEVRAANQRDVERARGAGLDSALLDRLQLGDDYIEQMAAGLEQLAAMPDP
                     IGTISDVRPMPSGIQVGRMRVPLGVIGIIYESRPNVTIDAAGLCIKSGNATILRGGSE
                     AIECNRLLASMISDALRVHGLPEDGVQLIATTDRAAVGLLVTMPEYVDIIVPRGGRGL
                     IERLMRESKVPMIKHLDGVCHVYIDDAADLEQAIRIADNAKTQRYGTCNTMETLLVGA
                     GIAPRVLPELGRVYAGKNVELRGCARSGALLQGAGIACIAATEDDWHTEYLAPILSIR
                     IVDGLDAAIEHINTYSSHHTDAIVTEDHTAAMRFLREVDSASVMINASTRFADGFEYG
                     LGAEIGISNDKLHARGPVGLEGLTSQKFVVFGHGEVRT"
     gene            4482..5924
                     /locus_tag="AC16HB_13040"
     CDS             4482..5924
                     /locus_tag="AC16HB_13040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5607758.1"
                     /translation="MKLLTTRWLVALLLALAAIRIASLGLYPLGDTTEARYAEVARLM
                     AATGDWITPHNDPGVPFWAKPPLSFWAQAGSIKLLGVNEFAARISSVLFAFATLALLW
                     QLARRAEPDHSTLEAWIALLAFATMPMAFLAAGAVMTDMALATTTTAAMVAFWIAWVQ
                     GSRPAGYAFFLALGLALLAKGPVGVVLVAASIGLFWLIEPARGANLKRLWHRLPWTGG
                     ILLMLAVAAPWYTAAEIRTPGFLDYFIVGEHIKRFLEPGWTGDRYGNAHIEPLGTIWY
                     FYAASVLTWLPVLAWLAWTRVRAHVALPRWTSFDRYLLAWALATPIFFTPARNIVWAY
                     VLPAMPALALLIARGCTRRLEHHAGGAVRWAVPATGIVATAAFVVAFFVVIPREWNDR
                     STRGLVEAKTHAEKEPGAPLLVIGTPPHSTKFYTRATYQRATLEQALAALERPGEVFI
                     LIDRRELKPQLQGATAEVAGTGRYVLLRKR"
     gene            5934..>6196
                     /locus_tag="AC16HB_13045"
     CDS             5934..>6196
                     /locus_tag="AC16HB_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR5607759.1"
                     /translation="MTAATQRFFAICPRGLEQLLADELRALGAQPAQPEPGGVAFAGT
                     LATAYAANLHSRLASRVLWEVCRRGYASEDDLYAAGHAVAWEA"
BASE COUNT          974 a         2092 c         2195 g          935 t
ORIGIN      
        1 gcgcgtgccg gtgtgggtcg gcaactacgt gctgatgggc tacggcgagg gcgcggtgat
       61 gggcgtgccc gcgcacgacg agcgcgactt cgcgttcgcg cagaagtacg ggctgccgat
      121 ccagcaggtg atcgcgatcg agggcgagac gttttcgctc ggcggctggc aggagtggta
      181 tgccgacaag cagcgcggcg tgtgcgtgaa ctccggcaag tacgacggcc ttggctacga
      241 ggccgcgacc gacgcgatcg ccgccgacct gaaagcgaag gctctgggcg acaagcagat
      301 ccagtaccgg ctgcgcgact ggggcatctc gcgccagcgc tactggggca cgccgatccc
      361 gatcatccac tgcgatgcct gcggctcggt gccggtgccg gagaaggatc ttccggtcct
      421 gctgccggag gacctggttc cggacggcag cggcaatccg ctggacaagg acgcgcgctt
      481 cctcgcgtgc aaatgcccgc agtgcggcca gccggcgcag cgcgagaccg acacgatgga
      541 cacgttcgtc gactcctcgt ggtactacat gcgctactgc tcgcccgatg cggcgacgat
      601 ggtcgatgcg cgcaacgact actggatgcc gatggaccag tacatcggcg ggatcgagca
      661 cgcggtgctg cacctgctct atgcgcgctt ctggaccaag gtgatgcgcg acctggggct
      721 ggtgcggttc gacgagccgt tcacgcggct gttcacccag ggcatgctgc tgaacgactg
      781 ctacttccgc gaagacgaat ccggccgcac acgctggttc tatccgaatg aagtcgaggt
      841 cagcttcgac gaccgtgggc gccccaccgg cgccatcgcg aaggaggacg gccagccggt
      901 gatgctcggt ggcatcgaga agatgtcgaa gagcaagaac aacgtggtcg agccacgcga
      961 catcatcgcg aaatttgggg ccgacaccgc gcgcgcgttc gtgatgttcg ctggtccgcc
     1021 cgacatgagc gcggcgtggt ccgactccgg cgccgagggg gtgtaccgct tcctgcggcg
     1081 gctgtggacc ttctgctacg cgcagcgcga cgcgatcaaa ggcgccgcct cgatcgacgg
     1141 cacggccctg cccgcgaagc tgcaggacgt gcggcgcgag ttgcacctgg cgctgaagca
     1201 ggcgctgttc gactaccagc gcatgcagta caacacggtc gtgtcggcca cgatgaagat
     1261 gctcaacgcg ctcgacgacg ccaggctcgg cgacgacgcc gcgagccgcg cgctcgcccg
     1321 cgagggaacg agcctcctgc tgcgcacgct gtacccgatc gcgccgcaca tcacgcacac
     1381 gctgtggacc gaactgggct tcgccgcggt gcacggtgat ctgctcgacg cagcgctgcc
     1441 cgccgtggat gaaggcgcgc tgagacagga cgagatcgag ctcgtcgtgc aggtcaacgg
     1501 caagctgcgc ggatcgatcc gcgtgccggc gcaggccgac aaggccgcga tcgagcgcgc
     1561 cgcgatggcc gacgccaacg tgcagaagtt cgtcgggacc aatccggtca agcgcgtcgt
     1621 cgtggtgccg aacaagctgg tcaacgtggt cgtgtgatgc gccgcgcgct gctggccgcg
     1681 atcgggctcg cgccgctcgc ggcggcgtgc gggtttcggc tgcgcggcgc gcgcgagttg
     1741 ccgttcgaaa cgatctacct cggcttcggc cccaactcgc cgctcggctc cgagctggcg
     1801 cgcaacatcc gcgccggaac cagcacgaag gtcgtcgaca atgccaagga cgcgcaggcg
     1861 gtgttcgaga ttctgagcca gagcacggac cgcgaagtgc tgtcggtcaa tgcacaggga
     1921 cgcgcgcgcg agttcacgct gcgccagcgg ctcaccttcc ggctgcacga cggcaaaggg
     1981 tcggaatatc tcggcccgac ccaggttgct gtgcagcgcg atgtcgcgtt cgacgacgcg
     2041 caggcactgg cgttcgaggc cgagaccgcc ctgctctacc gcgacatgca gaccgatctc
     2101 gtgcagcaga tcctgcgccg gctggcggcg gccaagggag tgcgcgggtg atcctgaagc
     2161 aagaggcgct cgccggccat ctggagcgcg ccgcgcatgc gggcaaactc gcgcgcatct
     2221 acacgatcgc cagcgatgag gcgctgcttt cgatcgaggc gcaggacgcg atccgcgctg
     2281 ccgcgcgccg cgccggcttc gccgagcgcg aagtgctgca cgccgacggg cgcttcgact
     2341 ggtccctgct gctgggcgcc acgacggggc tgtcgctgtt cgcggagaag aagatcgtcg
     2401 aggtgcgact gccgtcgggc aaaccgggca agtccggcgc cgaggcattg cgcgagcatg
     2461 cgagcaacgc gaccgacgcg acgctcgcga tcgtcgcgct gccgcggctc gaccgcgcca
     2521 cgcgcgaatc agcctgggcc gcggcgctgg agcacgcggg cgcgtggatc gacatcgcga
     2581 aggtcgagcg cgccgagctg ccagcctgga tcggccagcg gctggcgcgc cagcagcagc
     2641 gcgccgcgcg cgcggcgctg gagttcctcg ccgatcgcgt cgagggcaac ctgctcgccg
     2701 cgcatcagga gatcggcaag ctcgcgctgc tctatccgcc gggcgagatc acgctcgagc
     2761 agctgacgga atccgtgctg aacgtcgcgc gctacgacgt gttcgcgctg cccgccgcgc
     2821 tgtatgcggg ggactccgcg cgcgcggtgc gcatgctcga aggcctgcgc gcagagggcg
     2881 aggcgctgcc gctcgtgctg tgggcggtca gcgaggagat ccgcacgctg ctgaaggcga
     2941 agtccgcagc cgacgccggc cggccgcccg gcacgctggc gcgcgagttg cgaatctggg
     3001 ggccgcgcga aaagctgctg ccgcaggcgc tggcgcggct gaccgcagcc agcctttccg
     3061 cgctgctggc gcgctgcgcc gacgtcgacc gcctggtcaa gggcctgcgg gtcgagcggc
     3121 gcgactcgga cccgtggcta gaattgagcg acatcgcgct cgcgctcgcc acaggaagat
     3181 gaacgcacca caggaactgc gcacggatct ggcgtcctac atgaaggacg tcggaagccg
     3241 tgcgcgccgc gcgtccgccg cgatggcgaa ggccgccacc gcaaccaaga acgctgcgct
     3301 gacgagcctc gccgccgcga tacgcgcgcg ccgcgccgaa gtgcgcgccg ccaaccagcg
     3361 cgatgtcgag cgggcgcgcg gcgcgggcct cgacagcgcg ctgctcgacc ggctgcagct
     3421 cggcgacgac tacatcgagc agatggccgc ggggctggag cagctcgcag cgatgccgga
     3481 tccgatcggc accatcagcg acgtgcggcc gatgccgtcc gggatccagg tcggccgcat
     3541 gcgcgtgccg ctcggcgtga tcgggatcat ctacgagagc cggcccaacg tgacgatcga
     3601 tgcggcggga ctgtgcatca agagcggcaa cgcgacgatc ctgcgcggcg gctcggaagc
     3661 gatcgagtgc aaccggctgc tcgcatcgat gatttcggat gcgctgcgcg tgcacggcct
     3721 gccggaagac ggcgtgcagc tgatcgcgac caccgaccgc gccgcggtcg gcctgctggt
     3781 gacgatgccc gaatacgtcg acatcatcgt gccgcgcggc ggccgcggct tgatcgagcg
     3841 gctgatgcgc gagagcaagg tgccgatgat caagcacctc gacggcgtgt gccacgtcta
     3901 catcgacgac gctgccgacc tcgaacaggc gatccgcatc gccgacaacg ccaagacgca
     3961 gcgctacggg acctgcaaca cgatggagac actgctggtc ggcgccggca tcgcgccgcg
     4021 cgtgctgcct gagctcggcc gcgtgtacgc cggcaagaac gtcgaactgc gcggctgcgc
     4081 gcgctccggt gcgctgttgc agggcgccgg catcgcctgc atcgccgcga ccgaagacga
     4141 ctggcacacc gagtacctgg cgccgatcct gtcgatccgc atcgtcgacg gactcgatgc
     4201 agcgatcgag cacatcaaca cgtattcgag ccatcacacc gacgcgatcg tgaccgagga
     4261 tcacacggcg gcgatgcgct tcctgcgcga agtcgattcc gcatccgtga tgatcaacgc
     4321 gtcgacccgc ttcgccgacg gcttcgagta tgggcttggc gccgagatcg gcatttccaa
     4381 cgacaagctg cacgcgcgcg gaccggtcgg cctcgagggg ctgacgtcgc agaagttcgt
     4441 cgtcttcggg cacggcgaag tccgcacgta atccgcgctg aatgaaactg ctgacgaccc
     4501 ggtggctggt cgccttgctg cttgcgctcg ccgcgatccg cattgcgtcg ctcgggctgt
     4561 atccgctcgg tgacacgacc gaggcgcgct atgcggaagt cgctcgcctg atggccgcta
     4621 ccggcgactg gatcacgccg cacaacgatc cgggcgtgcc gttctgggcc aagccgccgc
     4681 tgtcgttctg ggcgcaggca ggatcgatca agctgctcgg cgtcaacgag ttcgccgcgc
     4741 gcatctcgtc ggtgctgttc gcgttcgcca cgctggcgtt gctgtggcag ctggcgcgcc
     4801 gagcggagcc ggatcactcg acgctcgagg cgtggatcgc actgctcgcg ttcgcgacga
     4861 tgccgatggc cttcctggcg gccggcgcag tgatgaccga catggcgctg gcgacgacga
     4921 cgaccgcggc gatggtcgcg ttctggatcg cgtgggtgca gggatcgcgc ccggccggct
     4981 atgcgttctt tctcgcgctc ggattggcgc tgctggcgaa agggccagtc ggcgtcgtgc
     5041 tcgtcgcggc gagcatcggt ttgttctggc tgatcgaacc cgctcgcggc gcgaacctga
     5101 agcgcctgtg gcaccggctg ccgtggaccg gaggcatcct gctgatgctt gccgtcgccg
     5161 cgccttggta cacggcagcg gaaatccgga cgccgggctt tctcgactac ttcatcgtcg
     5221 gcgagcacat caagcggttt ctcgagcccg gatggaccgg cgaccgctat ggcaacgccc
     5281 acatcgagcc gctcggaacg atctggtact tctatgccgc gtcggtgctg acctggctgc
     5341 cggtgctcgc atggctggca tggacgcgcg tgcgcgcgca cgttgcgctg ccgcgctgga
     5401 cctcgtttga ccgctatctg ctcgcatggg cgctcgcgac accgatcttc ttcacgccgg
     5461 cgcgcaacat cgtctgggcc tacgtgctgc cggccatgcc ggcgctggcc ctgctgatcg
     5521 cgcgcggctg cacacggcgg ctcgagcacc acgccggcgg cgcggtgcgt tgggctgttc
     5581 ccgcgaccgg catcgtcgcc acggcggcct ttgtggtcgc gttcttcgtc gtgatcccgc
     5641 gcgaatggaa cgaccgctcg acgcgcgggc tggtcgaggc gaagacgcac gctgagaaag
     5701 agcccggcgc gccgctgctg gtgatcggca cgccgccgca ctcgaccaag ttctacaccc
     5761 gcgcgactta ccagcgcgca acgctggagc aggcgctggc cgcgctggaa cgccccgggg
     5821 aggtgttcat tctgatcgat cgccgcgaac tgaaaccgca actgcagggc gccacggcgg
     5881 aggtcgccgg cacgggccgg tatgtgctgc tccgcaagcg ctgaggagcg gacgtgaccg
     5941 cagcgaccca gcgcttcttc gcgatctgcc cgcgcggcct ggagcagttg ctcgcggacg
     6001 aactgcgcgc actgggcgcg caaccggcgc agcccgagcc tggcggcgtg gcgttcgcag
     6061 gtacgctcgc caccgcgtac gcagccaatc tgcattcgcg cctggccagc cgtgtgctgt
     6121 gggaagtgtg ccgtcgtggc tacgcgagcg aggacgacct gtacgccgct gggcacgcgg
     6181 tcgcatggga ggcgca
//
LOCUS       DBNHBY010000534         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1049291, whole genome shotgun sequence.
ACCESSION   DBNHBY010000534 DBNHBY010000000
VERSION     DBNHBY010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1049291"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..640
                     /locus_tag="AC16HB_13050"
     CDS             158..640
                     /locus_tag="AC16HB_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HIR5607760.1"
                     /translation="MPTSLETLIRYRAAHADDPESRRVWLFHSHTYFDHAVPERVAEA
                     RAFMDLVRRTFASTAHVEVHSFIPFPAGPHPRGSFEVLFTREVFADYVSWLLFARPQT
                     LDILIHPLTRSQTLDHTARALWLGTALAIDRTLLEAVDARLLASGVTEESIIDGTKKH
                     "
     gene            complement(691..1653)
                     /locus_tag="AC16HB_13055"
     CDS             complement(691..1653)
                     /locus_tag="AC16HB_13055"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR5607761.1"
                     /translation="MSLPALLRDRLRLPVIGAPLFIVSNPTLVIEQCKAGIVGSFPAL
                     NARPESVLEEWLARITTELDGWRRAHPDATIAPYAVNQIVHASNTRLRHDVAACVQFK
                     VPIMITSLRAPDEVVQAAHGYGGLVFHDVTNIAHAKRALQAGVDGLILVCTGAGGHAG
                     RLSPFALVREVREFYDGTIILSGAIASGDAILAAQALGADLAYIGTRFIASQEANAPD
                     AYKQMLVDSRAEDIVYTNLFTGVHGNYLRGSIVAAGLDPDNLPTADKSQMNFGSGSSR
                     REKAWRDIWGAGQGAGQIHDVPATAAIVARLESEYRAARERLAV"
     gene            complement(1697..>2280)
                     /locus_tag="AC16HB_13060"
     CDS             complement(1697..>2280)
                     /locus_tag="AC16HB_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5607762.1"
                     /translation="DALKVNPLFDALPPYIQLRGSDPDVRRWLAPTVDFLRAEIDSGL
                     QGSRTILRRMADVLFIQALRAYAARNRAATGWLRGLGDPRVAKALVLMHQRYAEPWTL
                     DSLARAAGTSRTALAVQFMRLVGEAPMAYLARWRITRAANRLRTERISLTRVAESVGY
                     QSDAVFAKAFRRVTGVSPGRYRRAAAEAAAAPG"
BASE COUNT          357 a          792 c          785 g          346 t
ORIGIN      
        1 atcgccatcg agaaggagtg ggccgcacac ctgggcgcgc agcgattcga tgccctgcgc
       61 gagacgctgc tcgacctgtc gcgctggctg ggcaaactga cctgagaatg cgcgcgctcc
      121 cggtcccggc gaacacacgg cacgaaagga ccatgagatg ccgaccagcc tggaaacact
      181 gatccgctat cgcgccgcgc acgccgacga ccccgagtcg cgccgcgtgt ggctgttcca
      241 ctcccacacg tacttcgatc acgccgtgcc cgagcgggtc gcggaggcac gtgcattcat
      301 ggatctcgtc cggcgaacgt ttgcgtcgac ggcgcatgtg gaggtgcact cgttcattcc
      361 atttcccgcc ggaccgcacc cgcgcggaag cttcgaggtg ctcttcacgc gcgaggtctt
      421 cgccgactac gtgtcgtggc tcctgttcgc tcgcccgcag acgctcgaca tccttattca
      481 cccgctcacg cgctcacaga ccctcgatca caccgcgcgc gcgctctggc tcggcacggc
      541 gctcgcgatc gatcggaccc tgctggaagc ggtggacgcc cgcctgctcg cttccggcgt
      601 gaccgaagag tcgatcatcg acggcacgaa gaagcactga cagggcgact gcacttcgag
      661 ttgcggcagg ttcagccgat caatcgcccg tcaaacggcc agccgctcgc gcgccgcgcg
      721 gtactcactc tccagccgcg cgacgatcgc ggcggtggcc gggacgtcgt gaatctggcc
      781 ggcgccctga ccggcacccc agatgtcgcg ccacgccttc tcgcgccggc tgctgccgct
      841 gccgaaattc atctggctct tgtccgcggt cggcaggttg tcgggatcga ggcccgccgc
      901 gacgatgctg ccgcgcaggt agttgccgtg cacgccggtg aagaggttgg tgtagacgat
      961 gtcctcggcc cgggagtcga cgagcatctg cttgtacgcg tcgggggcgt tggcctcctg
     1021 cgaggcgatg aagcgcgtgc cgatgtaggc gagatcggcg ccgagcgcct gcgccgcgag
     1081 gatcgcgtcg cccgaggcga tcgcacccga caggatgatc gtgccgtcgt agaactcgcg
     1141 cacctcgcgc accagcgcga acggcgacag acggcccgca tggccgccgg cgccggtgca
     1201 cacgaggatc agcccgtcca cgcccgcctg caaggcgcgc ttcgcgtgcg cgatgttggt
     1261 cacgtcgtgg aacaccagcc cgccgtagcc gtgtgcggcc tgcacgactt cgtcgggtgc
     1321 acgcagcgag gtgatcatga tcggcacctt gaactgcacg caggcggcga cgtcgtgtct
     1381 gagccgcgtg ttcgacgcgt gcacgatctg gttcaccgcg tacggcgcga ttgtcgcgtc
     1441 gggatgcgcg cgccgccacc cgtcgagttc ggtcgtgatg cgcgcgagcc actcctccag
     1501 cacgctttcc ggccgcgcat tgagcgcggg aaaactgccg acgatgccgg ccttgcattg
     1561 ctcgatcacg agcgtcgggt tggagacgat gaacagcggc gcgccgatca cgggcaggcg
     1621 gaggcggtcg cgcagcaggg caggcagcga catcggatgc agtacgaggc agatcgcctc
     1681 gcactgtaat cgaagctcag ccaggcgcgg ccgcagcttc ggcggcagcg cggcggtagc
     1741 ggcccggcga aacgccggtc acgcgccgga atgccttggc gaacacggcg tcagactggt
     1801 agcccacgct ctccgccacg cgtgtcagcg atatgcgctc agtgcgcagc cggttggccg
     1861 cgcgcgtgat gcgccagcgc gccaggtacg ccatcggtgc ctcgccgacg agccgcatga
     1921 actgcaccgc cagcgcggtg cgcgaggtgc cggccgcgcg cgcgagcgag tcgagcgtcc
     1981 acggttccgc atagcgctgg tgcatcagca cgagcgcctt cgccacgcgc ggatccccga
     2041 gcccgcgcag ccatcccgtc gcggcacggt tgcgcgcggc gtatgcgcgc agcgcttgga
     2101 tgaacagcac gtcggccatg cggcgcagga tcgtgcgcga gccctgcagg ccagagtcga
     2161 tctcggcgcg gaggaaatcg accgtgggcg cgagccagcg ccgcacgtcg gggtccgatc
     2221 cgcgcagctg gatgtacggc ggcagcgcgt cgaacagcgg gttgaccttg agcgcatcgg
//
LOCUS       DBNHBY010000535         2640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1793643, whole genome shotgun sequence.
ACCESSION   DBNHBY010000535 DBNHBY010000000
VERSION     DBNHBY010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1793643"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16HB_13065"
     CDS             <1..352
                     /locus_tag="AC16HB_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5607763.1"
                     /translation="TAVFDAAGICIFTSFAWTLTDVQPQVAAACDGDWSLDKLNLVGE
                     RIWNLEKQFNLKAGLTKKDDDLPPRLKKEPAKTGPAKGLVNGLDKMLPEYYELRGWDG
                     EGVPKRETLTRLGL"
     gene            449..1699
                     /locus_tag="AC16HB_13070"
     CDS             449..1699
                     /locus_tag="AC16HB_13070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5607764.1"
                     /translation="MRHVILGNGPAGVVAAETLRQLAPRDEIVLVGAEREPPYSRMAI
                     PYLLMGHIGEQGTYLRKDADHYTRLNIQQLQGKAVRLDDRTRTIALESGQSLSYDRLL
                     IATGSHPIRPPVPGIDAPGVHTCWSLEDARAIAQKAGKGKRVLQVGAGFIGCIIMEAL
                     AERGVQLTVVEMGDRMVPRMMTPRAGNMIRRWVENKGVRVITGAKVDRIDAGAPLKAH
                     LSTGDTIETDLIIASAGVRPNTEFLQGSRVKIGQGVLVDNSMRTSVPEVFGAGDVVEA
                     AEFGTARGFVNAIQPNAVDQARVAALNMLGRPTQSQGAMAINVLDTLGLISSSFGQWS
                     GVPGGEGAEEVDDGAYRYLSLQFERDVLVGATAIGLTQHVGVLRGLIQNRTRLGGWKD
                     TLLGAPGRFVEAYVARSQPISITH"
     gene            1739..1990
                     /locus_tag="AC16HB_13075"
     CDS             1739..1990
                     /locus_tag="AC16HB_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5607765.1"
                     /translation="MNVTLKLFAMLSDYLPPDARNNRVQVELGDDATVGGLIDRYNLP
                     PRLTHLVLVNGAYVAPAARASTTLRDGDTVAIWPPIAGG"
     gene            2029..2307
                     /locus_tag="AC16HB_13080"
     CDS             2029..2307
                     /locus_tag="AC16HB_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607766.1"
                     /translation="MVIARDVTGTIAEFDAALRSAAAGCIAGAAPRYRIDMGAVQLEV
                     EVLVGPDRRIALLRLPTLLVTYRFLSGSRGQQQALLERLDRAMHRGGG"
     gene            2409..>2640
                     /locus_tag="AC16HB_13085"
     CDS             2409..>2640
                     /locus_tag="AC16HB_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607767.1"
                     /translation="MLAHRAGARVAARSRLRLRDEPTASLDPTAREALENRVREIRTD
                     GARIVMITHNLPQTAPRRTVSSRKAATSRGDRS"
BASE COUNT          435 a          863 c          925 g          417 t
ORIGIN      
        1 gacggccgtg ttcgacgcgg cgggcatctg catcttcacg tcgtttgcat ggacgttgac
       61 ggacgtgcag ccccaggtgg cagcggcctg cgacggcgac tggtcgctcg acaagctgaa
      121 cctggtcggc gagcgcatct ggaacctcga gaagcagttc aacctcaagg ccggcctgac
      181 caagaaggac gacgacctgc cgccgcggct caagaaggaa ccggccaaga ccggtccggc
      241 caagggactc gtcaacggcc tggacaagat gctgcccgag tactacgagc tgcgcggctg
      301 ggacggcgag ggcgtgccca agcgcgaaac gctcacgcgt ctgggcctgt agttggattg
      361 ccagcgcgcg ccgggcgtcg cgccggcgcg ctgcctggaa ctcaacccat cggggcgctg
      421 acggcgcccc gtttcttctc ggtgccacat gagacatgtc attctcggta acggtccggc
      481 gggcgtcgtg gccgccgaaa cgctgcgcca gctcgcaccg cgcgatgaga tcgtgctggt
      541 cggagccgag cgcgagccgc cctattcgcg catggcgatt ccgtatctgc tgatgggcca
      601 catcggagag cagggtacct acttgcgcaa ggatgcggac cactacacgc ggctgaacat
      661 ccagcagttg cagggcaagg cggtccgtct cgacgaccgg acgcgcacga tcgcgctgga
      721 gagcggccag tcgctgtcct acgaccgcct gttgatcgcc accggatcgc atccgatcag
      781 gccccccgtg ccgggcatcg atgcgcctgg cgttcacacg tgctggtcgc tcgaggatgc
      841 gcgcgccatc gcgcagaagg ccggcaaggg caagcgcgtg ctgcaggtcg gcgccgggtt
      901 catcggctgc atcatcatgg aggcgctggc cgagcgtggt gtgcagctga ctgtcgtcga
      961 gatgggcgac cgcatggtgc cgcggatgat gacgccgagg gccggcaaca tgatccgccg
     1021 ctgggttgaa aacaaagggg tgcgcgtgat caccggcgcg aaggtcgacc gcatcgacgc
     1081 cggcgcgccg ttgaaggcgc atctgtcgac cggcgacacg atcgagaccg atctcatcat
     1141 cgcttcagcc ggcgtgcgac cgaacaccga attcctgcag ggttcgcgcg tgaagatcgg
     1201 gcaaggcgtg ctggtcgaca actcgatgcg aacgtcggtg cccgaagtct tcggcgccgg
     1261 cgatgtcgtc gaagcggccg agttcggcac cgccagggga ttcgtgaacg cgatccagcc
     1321 gaacgcggtc gatcaggcgc gcgtggctgc gctcaacatg ctcgggcggc cgacgcagag
     1381 ccagggtgcg atggcgatca acgtgctcga tacgctggga ctgatctcat cgtcgttcgg
     1441 gcaatggagc ggtgtcccgg gcggcgaggg ggccgaagaa gtcgacgacg gcgcatatcg
     1501 ttacctgagc ctgcagttcg aacgcgacgt tctggtcgga gcgaccgcga tcgggctgac
     1561 gcagcacgtc ggcgtgctgc gcggcctgat ccagaaccgc acccggctgg gcgggtggaa
     1621 ggacacgctg ctcggcgcac ccgggcgctt cgtcgaggcg tacgtcgcgc gcagccagcc
     1681 gatttccatt acgcactgag ggcggtcgcg gtctggcggt tgccggtagc atcgcatcat
     1741 gaatgtcaca ctcaagctgt tcgcgatgct ctccgactac ctgccgcccg acgcgcggaa
     1801 caaccgtgtc caggtcgagc tgggcgacga cgcgacagtc ggcggcttga tcgatcgtta
     1861 caacctgccg ccgcggctga cgcatctcgt cctcgtcaac ggcgcttacg tcgctccggc
     1921 ggcgcgcgcc tcgaccacgc tgcgggacgg tgacacggtc gcgatatggc cgcccattgc
     1981 cggcggctga agcgaggatc ttcgcagcgc cccgaagtga cgtcgccgtt ggtcatcgca
     2041 cgggacgtca cgggcacgat cgcggaattc gacgctgcgt tgcggagcgc ggcagcgggc
     2101 tgcattgcgg gcgcggcgcc gcgctaccgc atcgacatgg gcgcggttca gctcgaagtc
     2161 gaagtcctgg tcgggccgga tcgccgcatc gccttgctgc gactgccgac actgctcgtc
     2221 acgtaccgtt tcctctccgg cagccgcggt cagcaacagg cgctgctgga gcgcctggac
     2281 cgcgcgatgc accgcggtgg cggatagcat gaaccgccgt cacgctcgcc tcggtcgcgc
     2341 cctgctcgcg gaacagtgat cggccgtagc ttgcctaggc ctttgcgctg ccggcgcgca
     2401 tcgacgccgt gctcgcgcat cgcgctggcg cgcgcgtggc cgcccggtcg cgcctgcggt
     2461 tgcgcgatga acccaccgcg agtctcgacc cgacggcgag agaagccctc gagaatcggg
     2521 tgcgcgagat ccgcaccgac ggagcgcgta tcgtgatgat tacgcataat ctgccgcaga
     2581 cggcgccgcg gagaaccgtt tcgtcgcgga aggctgcgac atcgcgcggc gatcgcagtg
//
LOCUS       DBNHBY010000536         8065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_575976, whole genome shotgun sequence.
ACCESSION   DBNHBY010000536 DBNHBY010000000
VERSION     DBNHBY010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_575976"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3247)
                     /locus_tag="AC16HB_13090"
     CDS             complement(<1..3247)
                     /locus_tag="AC16HB_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5607768.1"
                     /translation="MTDDLTAVDVRFDYQLTDNLTARRGQVFLTGTQALVRVLLMQRA
                     LDSARGLNTAGFVSGYRGSPLGMVDQQLWKASRLLREAHVEFLPAINEDLAATACLGT
                     QRVALDAKRKVDGVTAMWYGKGPGVDRSGDALKHGNAYGSSPLGGVLVVAGDDHGCVS
                     SSMPHQSDVAMQAWSMPILHPANVAEYLEFGLYGWALSRFSGNWIGFKAISEVVESAM
                     TVDLDRVPIDFDKPVDYTPPMNLHVHAVDLPSLAIEERLQHKLDAVRAFARVNSVDRH
                     VVSSPRATLGIVTVGKAHFDFMEVLRRLELDPDALAVAGVRVYKVGLVYPLEPTRVLD
                     FVQGLEEILVIEEKAPIVETQIKELLYPLPGIQRPRVSGKRDARTPLDQAPLLSATGE
                     LRPSRVMPVVAEWLARLNPALDRRHLVVDFTMPKLLSNEADGVLRVPYFCSGCPHNTS
                     TKLPEGSRALAGIGCHFMASWMNRETSGLIQMGAEGVDWAAHARFTNEKHVFQNLGDG
                     TYYHSGYLAIRQAIAAGATMTYKILYNDAVAMTGGQPVDGKIGVPQIARQVEAEGAQR
                     VVIVADDIGKYRGRAREFPQGTTFHHRGELDVVQRELRNTPGITVLIYDQTCAAEKRR
                     RRKKKEFPDPPRRIFINEQVCEGCGDCGEQSNCLSVIPVETDWGRKRRIEQSSCNKDY
                     SCVNGFCPSFVSVHGAVLRRHAGKEFTADDVTREIERMGAPPAWQWTGPFDLLIAGVG
                     GTGVVTVGALVTMAAHLEGKQSSVLDFLGFAQKGGAVLSFVRIAPTADLLNQVRIDTQ
                     QADVVLACDLVVGASQEALGTVKHGRTVIHVNTHEIATAAFVRDPDASLHTDALLAKM
                     RFAAGDDRVETVDAQAIAERLLGDTMPANIVMLGYAFQRGLVPVSEAALLRAIELNGV
                     AVEMNRTAFALGRIAAGAPHALRRFAAQDGDAPLRTPHRPPDWEDLDGDSGLIARRAQ
                     FLTEYQNVAYAQRYRALVARVRKAESERVGAPLKLTAAVARYAAKLMSIKDEYEVARL
                     YTDGRWLRELSTQFEHWDRLAFHMAPPLLVRPGPDGRPRKIEFGPWLHGALR"
     gene            complement(3316..3807)
                     /locus_tag="AC16HB_13095"
     CDS             complement(3316..3807)
                     /locus_tag="AC16HB_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR5607769.1"
                     /translation="MKQLLIVWHSRTGGAEQMARAAADAARAEIEIDVRLQHAQDTTG
                     DDVLAADGYLFVAPENLATMAVLMKEFFDRIYYAALEKINGRPYALLICAGSDGTGAV
                     RQIQRIATGLRLKEVAPPVIVNTAAQTPEAILAPKTIRASELARCREVAAALAAGLAA
                     GVF"
     gene            complement(3804..4631)
                     /locus_tag="AC16HB_13100"
     CDS             complement(3804..4631)
                     /locus_tag="AC16HB_13100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR5607770.1"
                     /translation="MLKGLLSRIGAARGVAIPDDLWETTLAALPFTARLTAEARGRLR
                     SLAERLLADKEMAGAGGLELTADMQVNIAVQACLPVLNLGIDWYRGWTSIVVYPSEFL
                     VPRAITDEAGVVHEYVEPIAGEAWDGGPLLLSWEDARTGRDEAGPYNVVIHEFAHKID
                     MLNGDADGVPPFDRKLHPKLAPEFWTSVLHDAVDRLSAELDLIERELPPGVDPDDAAA
                     DPFYAHLPLDPYAAHSEGEFFAVSSEAFFVEPAKLRDAFPQWYALLVEFFRQDPAIG"
     gene            4703..6835
                     /locus_tag="AC16HB_13105"
     CDS             4703..6835
                     /locus_tag="AC16HB_13105"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5607771.1"
                     /translation="MSAQPQLRETAVAIDPDAARAVVAPLYVDRKLRTGEPFLAHADG
                     MVAIVRPLRNDPDLLAAAYLFGVNDVLRDADDFVRARFGAGVAQLVVDLRVLMRLSER
                     TRGRANADAAAEAEALRKMLLAMVNDLRVVVLRLASRLQTLRWYAATKQASAEDFARE
                     TLQLYAPLANRLGIWQLKWELEDLAFRFLEPGTYKQIAHWLEAKRIEREAFITQSKER
                     LRQVLADAGIAAEVSGRPKHIYSIWSKMQAKSLAFEQLYDVRALRVIVAEVAQCYQAL
                     SLVHEHWTPVGSEFDDYIARPKPNGYRSLHTVVRDGQGRTLEIQIRTREMHEAAELGV
                     AAHWRYKEEGGARGKAARVEQERIAWLRQLLAWRADVAGPVGTGAVQEERIYVLTPQA
                     NVVELPAGATPIDFAYHVHTELGHRCRGARVDGAMVPLTTKLATGQTVEIVAAKSGGP
                     SRDWLNPELGYLASPRSRAKVRQWFNALELEQSVAAGREIVDRELARLGRTAVKLDDL
                     ARRLGFATTEELCVAATKEEFSLRSIEQALTVAPPPTIEEVPAALTGVGAGTAPAAKG
                     RVLVVGVDSLLTMLARCCRPVPPDEIIGFVTRGKGVSIHRANCVNARALGKRAPERLI
                     DVTWGRQADAAYPVDVFVLAQDRPGLLRDVSEVIAREKINVIGVNTQTRRGDAQMRFT
                     VEVPDVAALRRALAQLAEVAGVISARRR"
     gene            6870..6946
                     /locus_tag="AC16HB_13110"
     tRNA            6870..6946
                     /locus_tag="AC16HB_13110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6904..6906,aa:Val,seq:gac)
     gene            7071..>8065
                     /locus_tag="AC16HB_13115"
     CDS             7071..>8065
                     /locus_tag="AC16HB_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HIR5607772.1"
                     /translation="MISISLPDGSKKSFDGPVSAAEVAASIGAGLAKAAIAAKVDGKL
                     VDLSHVIERDAALAIVTDRDKEGLEVVRHSTAHLLAYAVKELFPDAQVTIGPVIDNGF
                     YYDFSYKRPFTPDDLAAIERKMTELAAQNEPVTREVWKRDDAVKFFESMGEKYKAEII
                     AAIPAQEDISLYRQGRFIDLCRGPHVPATGKLKVFKLMKVAGAYWRGDSRNEMLQRIY
                     GTAWAKKEDQDAYLKMLEEAERRDHRKLGRELDLYHFQEEAPGLIFWHPRGWAIWQSV
                     EQYMRQVYRDNGYQEVRAPQILDRSLWEKSGHWEKYRENMFTTESENRYYALKPM"
BASE COUNT         1352 a         2636 c         2763 g         1314 t
ORIGIN      
        1 tgcgcagggc gccatgcagc cagggtccga actcgatctt gcgcggccgt ccgtccggac
       61 ccggccgcac cagcagcggt ggcgccatgt gaaaggccag gcggtcccag tgctcgaact
      121 gcgtcgagag ttcccggagc cagcggccgt ccgtgtacag tcgcgcgact tcgtattcgt
      181 ccttgatcga catcagtttg gccgcgtaac gcgcgaccgc cgcagtgagc ttcagcggcg
      241 ccccgacgcg ctcgctttcc gcctttcgca cacgcgcgac cagcgctcga tagcgctgcg
      301 cataggcgac gttctgatac tcggtcagga actgcgcacg gcgcgcaatc aacccgctgt
      361 cgccgtccag gtcttcccag tcgggtgggc gatgcggcgt gcgcaatggc gcgtccccat
      421 cctgggcggc gaatcgtcgc agtgcgtgcg gcgcaccggc cgcgatgcgg ccgagcgcaa
      481 acgcggtgcg attcatctcg accgccacgc cgttcagttc gatcgcgcgc agcaatgccg
      541 cttccgacac cggtaccagc ccacgctgga acgcgtagcc gagcatcacg atgttcgccg
      601 gcatcgtgtc gccgagcagc cgttcggcga tcgcctgtgc gtcgacggtc tcgacgcgat
      661 cgtcgccggc agcgaaccgc atcttggcca gcagcgcgtc ggtgtgcaag ctcgcatccg
      721 gatcgcgcac gaatgcagcg gtggcgatct cgtgcgtgtt gacgtggatc accgtgcggc
      781 cgtgcttgac ggtgccgagt gcttcctggc tcgcgccgac gacaagatcg cacgccagca
      841 cgacgtcggc ctgctgcgtg tcgatgcgca cctggttcag caggtcagcg gtcggcgcga
      901 tgcgcacgaa tgacagcacc gcgccgccct tctgcgcgaa acccaggaag tcgagcacgc
      961 tggactgctt gccttcgaga tgcgcggcca tcgtcaccag cgctccaacc gtgaccacgc
     1021 cggtgccgcc cacgcccgcg atcagcaggt cgaacgggcc ggtccactgc cacgcgggcg
     1081 gcgcgcccat gcgttcgatc tcgcgcgtga catcgtcggc cgtgaattcc ttgccagcgt
     1141 gccggcgcag cacggcgccg tgcaccgaga cgaagctcgg gcagaagccg ttgacgcacg
     1201 aatagtcctt gttgcacgat gactgctcga tccggcgctt gcggccccag tcggtctcga
     1261 ccgggatcac cgacaggcag ttggactgct cgccacagtc gccacagccc tcgcagacct
     1321 gctcgttgat gaatatgcgg cgcggcggat ccgggaactc cttcttcttg cgccggcggc
     1381 gcttctcggc tgcgcaggtc tggtcgtaga tcaacacggt gatgccgggc gtgttgcgga
     1441 gttcgcgctg cacgacgtcg agttcgccgc gatggtggaa ggtcgtgcct tgagggaact
     1501 cgcgcgcgcg gccgcgatat ttcccgatgt cgtcggcgac gatcacgacg cgctgcgcgc
     1561 cctcggcctc gacctggcgc gcgatctgcg gcacgccgat cttgccatcg accggctgcc
     1621 cgccggtcat cgccaccgcg tcgttgtaga ggatcttgta ggtcatcgtc gcgccggcgg
     1681 cgatcgcctg acggatcgca aggtacccgg agtggtagta cgtgccgtcg ccgaggttct
     1741 ggaacacgtg cttttcattg gtgaaccgag catgcgcggc ccagtccacg ccttcggcgc
     1801 ccatctggat caggcccgaa gtctcgcggt tcatccagct cgccatgaag tggcaaccga
     1861 tgccggccag cgcgcgcgag ccttcgggga gcttggtcga cgtattgtgt ggacaaccgg
     1921 agcagaagta gggcacgcgc agcaccccgt ccgcttcgtt ggacagcagc ttcggcatcg
     1981 tgaaatcgac cacgaggtgg cggcggtcga gcgcgggatt caaccgcgcg agccattcgg
     2041 ccacgaccgg catcacgcgc gacgggcgca gttcaccggt cgcggacagg agcggcgcct
     2101 gatcgagcgg cgtgcgtgcg tcgcgcttgc ccgatacgcg cggccgctgg atgccgggca
     2161 gcggatagag cagttccttg atctgcgtct cgacgatcgg cgccttctcc tcgatcacga
     2221 ggatttcctc gagaccctgc acgaagtcga ggacgcgcgt gggctcgagc ggatacacga
     2281 gtccgacctt gtacacgcgc acgcccgcga ccgcgagcgc atcgggatcg agttcgagcc
     2341 ggcgcagcac ctccatgaag tcaaagtgcg ctttgccgac cgtgacgatg ccgagcgtgg
     2401 cgcgcggact cgacaccacg tggcggtcga ccgagttgac gcgcgcgaac gcgcgcacgg
     2461 cgtcgagctt gtgctgcagg cgctcctcga tcgcgagtga tggcaggtcg acggcgtgca
     2521 cgtgcaggtt catcggcggc gtgtagtcga ccggcttgtc gaaatcgatc ggcacgcgat
     2581 cgagatcgac cgtcatcgcc gactcgacca cctccgagat cgccttgaag ccgatccagt
     2641 tgccggagaa acgcgacagc gcccagccgt acaagccgaa ctcgagatac tcggcgacgt
     2701 tggccggatg caggatcggc attgaccacg cctgcatcgc gacgtccgac tggtgcggca
     2761 tcgacgacga cacgcagccg tggtcgtcac ccgccacgac cagcacgccc ccgagcgggc
     2821 tggagccgta tgcgttgcca tgcttgagcg catcgccgga gcggtcgaca cccggaccct
     2881 tgccgtacca catcgccgtc acgccatcga ccttgcgctt tgcatcgagc gcgacgcgct
     2941 gcgtgccgag gcaggcggtg gcggcgagat cctcgttgat cgcaggcaga aactcgacgt
     3001 gtgcttcccg cagcagcctg gatgccttcc acagttgctg gtcgaccatg ccgagcggac
     3061 ttccgcgata gccggaaacg aagcccgccg tgttcagccc gcgcgcgctg tcgagggcgc
     3121 gctgcatcag cagcacccgc accagcgcct gcgtccccgt caggaatacc tgaccgcgac
     3181 gggcggtcag gttgtcggtg agctggtagt cgaaacgaac gtcgaccgcg gtgagatcgt
     3241 cggtcatgga cgcactcccg agcgaaagtg cggcgagctt agccagttgc gcggacggcg
     3301 caacaacagc cccactcaga acacgccggc ggccaggccg gcggccagcg cggcggcgac
     3361 ctcgcggcag cgcgcgagtt cgctcgcccg aatcgtcttc ggcgccagga tcgcctccgg
     3421 cgtctgcgcc gcggtattca cgatgaccgg tggcgcgact tccttcaacc gtagcccggt
     3481 ggcgatgcgc tggatctgcc gcaccgcgcc ggtgccgtcg gagccggcgc agatgagtaa
     3541 tgcgtacggg cggccgttga tcttctccag cgccgcgtag tagatgcgat cgaagaactc
     3601 cttcatcaga acagccatcg tcgccaggtt ttccggtgcg acgaacaggt atccatcagc
     3661 ggcgagcacg tcgtcgccgg tggtgtcctg cgcgtgttgc agtctcacgt cgatttcgat
     3721 ctccgcgcgc gccgcgtcgg ccgcggcccg cgccatctgc tcggcgccgc cggtgcgcga
     3781 atgccagacg atcagcagtt gtttcatccg atggcgggat cctgccggaa gaactcgacc
     3841 agcagcgcat accactgtgg aaacgcgtcg cgcagcttcg ccggttcgac gaaaaacgct
     3901 tcgctcgaca ccgcgaagaa ttctccctcg ctatgcgccg cgtacggatc cagcggcagg
     3961 tgcgcgtaga acggatcggc ggcggcgtcg tccggatcga cccccggcgg cagttcgcgc
     4021 tcgatcagat cgagctccgc gctcaggcgg tcgactgcgt cgtgcagcac gctggtccag
     4081 aattccgggg cgagcttggg gtgcaacttg cggtcgaacg gcggcacgcc gtcggcgtcg
     4141 ccgttcagca tgtcgatctt gtgcgcgaac tcgtgaatca cgacgttgta cggacccgcc
     4201 tcgtcgcggc ccgtgcgcgc gtcctcccat gacaacagca gcgggccgcc gtcccatgct
     4261 tcgcctgcga tcggctcgac gtactcatgc accacgccag cctcgtcggt gatcgcgcgc
     4321 ggcaccagga actcgctcgg atacacgacg atgctggtcc agccgcgata ccagtcgatg
     4381 ccgaggttca gtaccggcag gcatgcctgc acggcgatat tcacctgcat gtcggctgtc
     4441 agttcgagtc cgccggcgcc ggccatttcc ttgtccgcca gcaaccgctc ggcaagggag
     4501 cgcagccggc cgcgggcttc cgcggtcagc cgtgcggtga atggcaatgc cgcgagcgtt
     4561 gtttcccaga gatcgtcggg aatcgccacg ccgcgcgcgg cgccaatccg cgacagcaat
     4621 cccttcaaca ttcattgagt gtgccgcaag cggcgccgcg atccgcattc ctataatcga
     4681 ccggccgttc tagtcattcc cgatgtcagc ccaaccccag ctgcgcgaaa ccgcggtggc
     4741 gatcgatccc gacgctgcgc gcgcggtggt ggcgccgctc tacgtcgacc gcaagctgcg
     4801 caccggcgaa ccatttctcg cgcacgctga cggcatggtc gcgatcgtgc gcccattgcg
     4861 aaacgacccg gatctgctgg ccgccgcgta cctgttcggc gtcaacgacg tgttgcgcga
     4921 cgccgacgat ttcgtgcgtg cgcgtttcgg cgccggcgtt gcgcagctgg tggttgactt
     4981 gcgcgtgctg atgcggctgt cggagcgcac acgcggccgt gcgaatgcgg atgcggcggc
     5041 cgaggccgag gcgctgcgca agatgctgct cgcgatggtc aacgatctcc gcgtcgttgt
     5101 cctgcggctg gcgtcgcgcc tgcagacgct gcgctggtac gcggcgacca agcaggccag
     5161 cgcggaggac ttcgcgcgcg agacgctgca gctgtacgcg ccgctcgcga accgcctcgg
     5221 catctggcag ctgaagtggg agctggagga tctcgcgttc cggtttctcg agccgggaac
     5281 gtacaagcag atcgcgcatt ggctggaggc aaagcgtatc gagcgcgaag cgttcattac
     5341 gcagtcgaag gagcgcctgc ggcaggtgct cgcggacgcg ggcatcgcgg ccgaggtcag
     5401 cggccggccc aagcacatct acagcatctg gagcaagatg caggcgaaat cgctcgcgtt
     5461 cgagcagctg tacgacgtgc gcgcgttgcg tgtgatcgtc gcggaagtcg cgcagtgcta
     5521 ccaggcgctc tcgctggtgc acgagcactg gacgccggtc ggcagcgagt tcgacgatta
     5581 catcgcgcgc ccgaagccca acggctaccg gtcgctgcat acggtcgtgc gcgacggtca
     5641 gggccgcacg ctcgagattc agattcgcac gagagagatg cacgaggcgg ccgagctcgg
     5701 cgtcgcggcg cactggcgct acaaggagga gggtggcgcg cgcggcaagg cggcgcgcgt
     5761 cgagcaggaa cgcattgcgt ggctgcggca gctgctcgca tggcgcgccg acgttgcggg
     5821 accggtcggg acgggtgcgg tgcaggagga gcgcatctac gtcctgacgc cgcaggcgaa
     5881 cgtggtcgaa ctgccggcgg gcgccacgcc gatcgatttc gcctaccacg tgcacaccga
     5941 gctgggacac cggtgccgcg gtgcgcgcgt cgacggcgcg atggttccgc tgacgaccaa
     6001 gctcgcgacc ggccaaacgg tcgagatcgt cgccgccaag tcgggtggac cgagccgcga
     6061 ctggctcaat cccgagctcg gctatctggc cagcccgcgt tcgcgcgcca aggtgcggca
     6121 gtggttcaac gcgctcgaac tcgagcaaag cgtcgccgcc gggcgcgaga tcgtcgatcg
     6181 cgagctggcg cgcctgggca ggacggccgt caagctcgac gatctggcgc gccggctcgg
     6241 attcgcgacc acggaggaac tgtgcgtggc ggcgaccaag gaggagttca gcctgcgcag
     6301 catcgagcag gcgctgacgg tggcgccgcc gcccacaatc gaggaagttc cggcggcgct
     6361 gactggcgta ggcgcgggga cagctccggc cgcaaagggc cgcgtgctgg tggtcggcgt
     6421 cgattcgctg ctgacgatgc tggcgcgctg ctgccgccca gtgccgcccg atgaaatcat
     6481 cggcttcgtc acgcgtggca agggtgtgtc gattcatcgg gccaactgcg tgaatgcgcg
     6541 tgcgctgggc aagcgcgcgc ccgaacggct gatcgacgtg acctggggca ggcaggccga
     6601 cgcggcctat ccggtcgatg tcttcgtcct ggcgcaggat cggccggggc tgctgcgcga
     6661 cgtctcggag gtcatcgcgc gcgagaagat caacgtgatc ggcgtcaaca cgcagacccg
     6721 gcgcggcgac gcgcagatgc ggttcacggt cgaggtaccg gacgtggccg cgctgcgacg
     6781 cgcgctggcg cagctcgccg aggttgccgg cgtgatttca gcgcgccgac gctgattccg
     6841 gcggcgctcg tgtatcattc cgcggactca ggcgcgtagc tcagctggtt agagcaccac
     6901 cttgacatgg tgggggtcgg tggttcgagt ccactcgcgc ctaccaccgc aacgaaggga
     6961 aattggcaat gacgtttcga actacgacga ccggaagttg atcggcccgg acgagttcgc
     7021 ccttcgaaaa agtgcggctc gaccgcactt tttttttgct ggagatgacg atgatttcga
     7081 tttccctgcc ggacggctcg aagaagtcgt tcgacggacc cgtgagcgcg gccgaggtgg
     7141 ccgcgtcgat cggggcagga ctggccaagg ccgcgatcgc ggccaaggtc gacggcaagc
     7201 tcgtcgatct gtcgcatgtg atcgagcgcg acgcggcgct ggcgatcgtg acggaccggg
     7261 acaaggaagg cctggaggtc gtacggcatt cgacggcgca tctgctcgcg tacgcggtca
     7321 aggagctgtt tcccgacgcc caggtgacga tcgggccggt gatcgacaac ggcttctatt
     7381 acgacttctc gtacaagcgc ccgttcacgc cggacgatct ggcggcgatc gagaggaaga
     7441 tgacggaact cgctgcgcag aacgagccag tcacgcgcga agtgtggaag cgcgacgacg
     7501 cggtgaagtt cttcgagtcg atgggcgaga agtacaaggc cgagatcatc gccgcgatcc
     7561 cggcgcagga agacatatcg ctgtaccggc aaggcaggtt catcgacctg tgccgcggcc
     7621 cgcacgtgcc ggcgacgggg aagctgaaag tcttcaagct gatgaaggtt gccggtgcgt
     7681 actggcgcgg cgacagccgc aacgagatgc ttcagcggat ctacggcacg gcctgggcca
     7741 agaaggagga ccaggatgcg tacctgaaga tgctggagga ggcggagcgg cgcgaccacc
     7801 gcaagctggg acgcgagctc gacctgtatc acttccagga agaggcgccg gggctgatct
     7861 tctggcatcc gcgcggctgg gcgatctggc agtcggtgga gcagtacatg aggcaggtgt
     7921 atcgcgacaa cggctaccag gaggtgcgcg cgccacagat cctcgaccgc tcactgtggg
     7981 agaaatcagg gcactgggag aagtaccgcg agaacatgtt cacgaccgag tcggagaacc
     8041 ggtactacgc gctgaagccg atgaa
//
LOCUS       DBNHBY010000537         7317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1455761, whole genome shotgun sequence.
ACCESSION   DBNHBY010000537 DBNHBY010000000
VERSION     DBNHBY010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1455761"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16HB_13120"
     CDS             complement(<1..236)
                     /locus_tag="AC16HB_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR5607773.1"
                     /translation="MQQTPRVIAHLDMDAFYASVELLRYPELRGLPVVIGGGARHQPV
                     WETDHATGEQRRRFARLRDYAGRGVITTATYEAR"
     gene            377..841
                     /locus_tag="AC16HB_13125"
     CDS             377..841
                     /locus_tag="AC16HB_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607774.1"
                     /translation="MRTLRSAAALTAVAAVALAATSALEGCSVGGGDGGPAPASYALR
                     IDGPDTVSTTQDFIVLVGEGFLPAGSTCPGGCEGLLPPPVFGNLGSYTLTWSNAANGQ
                     SGPILLRWICNCGGAVPSWNATIALAPGANRITVTETDRDRTQQASMLVTRP"
     gene            complement(849..1391)
                     /locus_tag="AC16HB_13130"
     CDS             complement(849..1391)
                     /locus_tag="AC16HB_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HIR5607775.1"
                     /translation="MPATSVDSNRRRALAALAALGALALPFAPRAWAAAPRSLSFYHT
                     HTGERLSVVYFEDGAYMPDALASLDALLRDFRTGDRHPIDARLFDTLHALNLACGPGT
                     FEIISAYRSPRTNALLHAHSNGVATNSLHLQGRAIDVRLTGRDTRRLRDAALALGAGG
                     VGYYAASDFVHVDTGRPRTW"
     gene            1415..3073
                     /locus_tag="AC16HB_13135"
     CDS             1415..3073
                     /locus_tag="AC16HB_13135"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5607776.1"
                     /translation="MKSSRIVTRRTQWFDSRVWRIALGMLLGVAVLAASAAPPVEALR
                     ARVEALRTHDDARIGAGSIAARNLVAALYERRGFAPLWAEPERVRRLRAALQDSATHG
                     LDPRDYHVEALTPGAASEVEHELLLTDAFVRLAYHLYFGKADPRALHGGWNFARTLGG
                     VEPLTALAPLIEARDPAAALEGLAPRVPMYRDLRAALEDLRRIERAGGWRAIASGPKL
                     EPGVSDVRVAQLRERLRASGDLAAGADTSAFDATLAAAVRRFQSRHGLEPDGVVGKST
                     LEALNVPVAERIGQVRVNLERLRWVARDLEGDFLLVDIAGFGAQLWLDGRLAWSSRVV
                     VGRPYRTTPVFRARMKYLVLNPEWNVPPTILREDVLPKAVRDPGYLGKHDMRVLDRAG
                     RPLDAAAIDWSQYRGEPRTFPHQIVQAPGRENPLGRIKFMFPNEHSVYLHDTPSRALF
                     DKTVRAFSSGCIRIEKPLELALLLLDDRERWSEANLREAIATGHTQTVPVVRAVPVLL
                     LYFTANAADGALQFRPDLYGRDRAVLVALAAPFRFAPVDRGHPR"
     gene            complement(3070..3879)
                     /locus_tag="AC16HB_13140"
     CDS             complement(3070..3879)
                     /locus_tag="AC16HB_13140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5607777.1"
                     /translation="MTRSGSKASPTALITGASAGIGAALARCFARAGHRVVLVARSAD
                     KLRALARTLRAEFGVEALPLAADLAQPRAATKLAARLRRRRVAIDILVNNAGVLEQGP
                     FVRIPAQRHLQLIDLNVGALTAMLAQFVPPMVARGRGRVLNVASIAAFQPVPTLALYA
                     ATKAFVLSLSESLAEELRDSGVTVTALCPGITATDMLANATRHHARLARLPGFVVGDV
                     DAVAAEGYRACMNGDAIAVPGVVNRATTTAARAAPRWLVRRVGGALGRKLL"
     gene            4041..5231
                     /locus_tag="AC16HB_13145"
     CDS             4041..5231
                     /locus_tag="AC16HB_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5607778.1"
                     /translation="MRSCISRLAAAVSAAAILVSCATVAPSAGKPEYMLVGIDNKVVF
                     ADDGSLKLLPPGKDLVSIVDIGTDPARPRIVASLPLMNSVVGPPTNLAITPDGALGLV
                     ANSLEWMQDGAGWKFVPDNKLYVLDLTANPPRHIDTVAVGKQPSGLSINGAGNLALIA
                     NRGDNSISVLSIQGKQVKLIDTVAMGEQVSAVVFTPDGKRAMAAKFPGHKLAPLNVDG
                     QKVTYDKRDVPVGLWPYNLGVTTDGKLVLTPDNGNSGRSDGHVDTVTVVDIEANPPRA
                     IDKVVVGDSPEGFAVNPNGRMAAAILLGGSDGPKNAWFYKRNGAVVTLRIDGKKVTKT
                     GEVQVAGVPEGAVFSPDGNWLYVGNYADNSISVLRVHGDTVVDTGVVVSLPGSPASMR
                     GRVQ"
     gene            complement(5299..>7317)
                     /locus_tag="AC16HB_13150"
     CDS             complement(5299..>7317)
                     /locus_tag="AC16HB_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR5607779.1"
                     /translation="TMTHASHPPNRLARETSPYLLQHAHNPVDWYPWGEEAFAKARAE
                     DRPIFLSVGYATCHWCHVMERESFEDESIAQLLNEGFVPIKVDREELPDVDHVYMTAL
                     QALTQHGGWPMNMFLTPQLAPFFGGTYFPPLPRYGMPSFADVLRAVQQAWGARRSELE
                     QQAGALLDHVRRGIELPRRAPPADPHQRAVETLAKTFDSAHGGFGGAPKFPQPPLLQY
                     LLTLSAIGSERARAMLTFTLGHMAAGGMYDQVGGGFARYSTDERWHVPHFEKMLYDNA
                     QLARVYAGAYRLTGDARLRFVAEDTLAYLRREMHPAAARAAFSSAQDADSEGVEGKFY
                     VWTAAELRALLGADADAALRLYGATAEGNWEHGVNVLERRDPEPARAALGLTPDEFAR
                     WERSVRDRLYAVRARRVWPITDDKVLTDWNGMALRAFAEAGRLLERADLVQTAVALAR
                     FLLDTMCGDGRLRHAWRAGTLRAESFLSDCALVGLGLVELHAATGEVDWLHAARDLAA
                     QMIERFHVADEGFYDGEPGHLPVRARDLFDGALPSGTAAACELLLRLAGVYERGDWAD
                     LVQQTLERHGALLEHPSAAPALLQVHLLNQRGGELALPAPAGALGAARAAFAPLVTIV
                     NAAPGSVPLLADRVAGQAYLCRHGTCQLPAHTLDDLRSQLDRLHAPVP"
BASE COUNT         1129 a         2511 c         2588 g         1089 t
ORIGIN      
        1 ctgcgcgcct cgtaggtggc ggtggtgatc acgccgcgcc ccgcatagtc gcgcagccgc
       61 gcgaagcggc ggcgctgctc gccggtcgcg tgatcggtct cccacaccgg ctggtgccgc
      121 gcaccgccgc cgatcacgac cggcagaccg cgcagttccg gatagcgcag cagttcgacc
      181 gatgcgtaga acgcgtccat gtcgaggtgc gcgatcacgc gaggagtctg ttgcatgcgg
      241 gttctgcgca cggtgtcagc aagcgacgtc gcggcgcgcg gcgagtctaa gacgccaccg
      301 acctgatccg gggcaacaaa tgccgcgcgg catcctgtcc aatcggagtc aaatgcttcg
      361 acccgggcag gcaacgatgc gcaccctgcg atccgcggcc gcgttgaccg ccgtcgccgc
      421 ggttgcactg gctgcgacca gcgcgctcga gggatgcagc gtcggcggcg gcgacggcgg
      481 gccggcgcct gcgtcatacg cattgcgcat cgacggcccg gacaccgtca gcacgacgca
      541 ggatttcatc gtgctggtcg gcgaaggatt cctgcccgcg ggttcgacct gccccggcgg
      601 ctgcgaaggg ctgttgccgc ccccggtatt cgggaacctc ggcagctaca cgctgacgtg
      661 gtccaacgcg gccaacgggc agagcggccc gatcctgctg cgctggatct gcaactgcgg
      721 cggagccgtg ccatcctgga acgccacgat cgcgctggcg cccggcgcaa accgcatcac
      781 ggtgaccgag accgaccgcg atcgcacgca gcaggcatcg atgctcgtga cgcggccgtg
      841 atgcgcgctt accaggtgcg cggtcgcccg gtgtcgacgt gcacgaagtc ggacgccgcg
      901 tagtagccga cgccgccggc gccgagcgcg agcgccgcgt cgcggagcct gcgcgtgtcg
      961 cggccggtca ggcgcacgtc gatcgcgcgt ccctgcaggt gcaggctgtt ggtcgccacg
     1021 ccgttgctgt gcgcatgaag tagtgcgttg gtgcgcggcg agcggtacgc cgagatgatc
     1081 tcgaacgtgc ccggcccgca cgcgaggttc agcgcgtgca gcgtgtcgaa cagccgcgcg
     1141 tcgatcgggt gccggtcgcc ggtgcggaag tcgcgcagca gcgcatcgag cgacgccagc
     1201 gcgtccggca tgtacgcgcc gtcttcgaaa tagaccacgc tcaagcgctc gcccgtatgc
     1261 gtgtgataga aggaaagcga tcgcggcgcc gcggcccatg cgcgcggcgc gaacggcagc
     1321 gccagcgcac cgagcgcggc gagcgcggcg agcgcgcggc gacggttcga atcgacagag
     1381 gttgctggca cggcgcaggc ggatcgaaga acggatgaaa agttcgcgca tcgtaacccg
     1441 gcgcacgcaa tggttcgact cacgcgtttg gcggatcgcg ctggggatgc tgctcggcgt
     1501 cgccgtgctc gcggcatcgg cagcgccgcc ggtcgaggcg ctgcgcgccc gcgtggaggc
     1561 cttgcgcacg cacgacgacg cgcgcatcgg cgccggctcg atcgccgcgc gcaatctggt
     1621 tgccgcgctg tacgagcggc gcggcttcgc accgttgtgg gccgagccgg agcgcgtgcg
     1681 ccggctgcgc gccgccttgc aggacagcgc gacgcacggc ctcgacccgc gcgactacca
     1741 cgtggaagcg ctcacgccag gcgcggcgag cgaggtcgag cacgaactgc tgctgaccga
     1801 cgcgttcgtg cggctcgcct accacctgta cttcggcaag gccgatccgc gcgctttgca
     1861 tggcggctgg aacttcgcgc gtacgctggg cggtgtcgag ccactgacgg cgcttgcacc
     1921 gctgatcgaa gcgcgcgatc cggccgccgc gctcgaaggg ctggcgccgc gcgtgccgat
     1981 gtaccgcgac ctgagggccg cgctcgagga tctgcgccgc atcgagcgcg ccggcggctg
     2041 gcgcgcgatc gcgtcgggac cgaagctcga gccaggcgtg agcgacgtcc gcgtggcgca
     2101 gttgcgcgag cgcttgcgcg cgagcggcga tctggccgcg ggcgccgata cgtccgcctt
     2161 cgacgcaacg ctggcggcag ccgtgcggcg cttccagtcg cgccacgggc tggagcccga
     2221 cggcgtggtc ggcaagtcga cgttggaagc gctgaacgtc ccggtcgccg agcgcatcgg
     2281 gcaggtgcgc gtgaacctcg aacggctgcg ttgggtcgcg cgcgatctgg aaggcgattt
     2341 cctgctggtc gacatcgccg gtttcggtgc gcaactgtgg ctcgatggcc ggctcgcgtg
     2401 gtcgtcgcgc gtggtcgtgg ggcggccgta tcgcaccacg ccggtgttcc gcgcgcggat
     2461 gaaatacctg gtgctgaatc ccgagtggaa cgtgccgccg acgatcctgc gcgaggacgt
     2521 gctgccgaag gcggtcaggg atccgggcta tctcggcaag cacgacatgc gcgtactcga
     2581 ccgcgcgggg cggcccctcg acgcggcagc gatcgactgg tcccagtacc gcggcgagcc
     2641 gcgcacgttc ccgcaccaga tcgtgcaggc gccggggcgc gagaatccgc tcggccgcat
     2701 caagttcatg ttccccaacg agcactcggt gtacctgcac gacacaccgt cccgggcgct
     2761 gttcgacaag acggtgcgtg cgttcagctc gggctgcatc cggatcgaaa agccgctcga
     2821 actcgcgctg ctgctgctcg acgatcgcga acgctggtcg gaggcgaacc tgcgcgaggc
     2881 gatcgccacc ggtcacacgc agacggtgcc ggtcgtgcgt gcggtgccgg tgctgctgct
     2941 gtacttcacc gccaatgccg ccgacggcgc gctgcaattc cggcccgacc tctacggccg
     3001 cgaccgtgcg gtgctggtcg cgctggccgc accgttccgt ttcgcgccgg tcgatcgcgg
     3061 ccatccgcgt taaaggagct tgcggccgag cgcgccgccg acgcgccgga cgagccatct
     3121 gggcgcggcg cgggcggccg tggtggtcgc gcgattgacg acgccgggga ccgcgatcgc
     3181 gtcgccattc atgcacgcgc gatagccctc ggcggcgacc gcgtcgacgt cgccgacgac
     3241 gaagcccggc agccgcgcca ggcgcgcgtg gtgccgtgtc gcgtttgcca gcatgtcggt
     3301 ggcggtgatt cccgggcaca gcgcggtgac ggtgacaccg ctgtcacgca attcctcggc
     3361 cagcgattcc gacagcgaca gcacgaacgc cttggtcgcg gcgtacagcg ccagcgtcgg
     3421 caccggctgg aacgcggcaa tcgacgcgac attgagcaca cgcccgcgcc cgcgtgcgac
     3481 catcggcggg acgaactgcg ccagcatcgc cgtcagcgcg ccgacgttca ggtcgatcaa
     3541 ttgcaggtgg cgctgcgcgg gtatgcgcac gaacggcccc tgctcgagca cgccggcgtt
     3601 gttcacgaga atgtcgatcg ccacgcgccg gcggcgcagt cgcgcggcca gtttcgttgc
     3661 tgctcgaggt tgcgcaaggt cggccgccaa cggcagcgcc tcgacgccga actccgccct
     3721 gagcgtccgc gccagcgcgc gcagcttgtc cgcgctgcgc gcgaccagca ctacgcggtg
     3781 accggcccgc gcaaagcagc gggcgagcgc cgcgccgata ccggccgagg cgccggtgat
     3841 gagcgccgtg ggtgatgcct tcgagccaga acgagtcatg tcgtgtcctt ccgatgcctg
     3901 ctccatctgg aggcgcgaag cactcacgcc tttccgtgaa tgcattgtcc gtcatggcgt
     3961 ccctcgcggc agagcgggta aagtcagact cgcgcttcgt gttcgccgat gcgcagtcaa
     4021 gaacattggg ggagacatcc atgcgttcgt gcatttcgcg ccttgcggcg gccgtgtccg
     4081 cggccgcaat tctcgtgtcc tgcgccaccg tcgcaccgtc ggccggaaag ccggagtaca
     4141 tgctcgtcgg catcgacaac aaagtggtat tcgccgacga cggcagcctg aagcttcttc
     4201 cgccgggcaa ggacttggtg agcatcgtcg atatcggcac cgaccctgcc aggccgcgaa
     4261 tcgtcgcgag cctgccgctg atgaactcgg tggtcggtcc gcccacgaat ctcgcgatca
     4321 cgcccgacgg agcgctcgga ctggtggcga actcgctcga gtggatgcag gacggcgccg
     4381 gctggaagtt cgttcccgat aacaagctct acgtgctcga cctgaccgcc aatccgccgc
     4441 ggcacatcga tacggtcgcg gtcggcaagc agccgtcggg actttcgatc aacggtgccg
     4501 ggaatctcgc gctgatcgcc aatcgcggcg acaactcgat cagcgtgctc tcgatccagg
     4561 gcaaacaggt caagttgatc gacacggtgg cgatgggcga acaggtttcg gcggtggtct
     4621 tcacgccgga cggcaagcgc gcgatggcag cgaagttccc cgggcataag ctggcgccgc
     4681 tgaacgtcga cgggcagaag gtgacttatg acaagcgcga tgtccccgtc ggtctgtggc
     4741 cctataacct gggcgtcacg accgacggaa agctggtgct gacccccgac aacggcaaca
     4801 gcggtcgctc ggatggccac gtcgacacgg tcaccgtggt cgacatcgaa gcgaatccgc
     4861 cgcgcgcaat cgacaaggtc gtcgtcggcg attcgccgga gggattcgcg gtgaacccga
     4921 acggcaggat ggctgcggcg atattgctgg gtggatcgga cgggccgaag aatgcgtggt
     4981 tctacaagcg caacggcgcg gtcgtgacgc tgaggatcga cggcaagaaa gtcacgaaga
     5041 ccggcgaagt gcaggtcgcc ggcgtcccag agggcgccgt gttcagcccg gatggcaact
     5101 ggctgtatgt cggcaactat gccgacaaca gcatctcggt gctgcgcgtc cacggcgaca
     5161 ccgtcgtcga caccggcgtc gttgtctcgc tgccgggaag cccggcttcg atgcgcgggc
     5221 gcgtgcagta ggcgaatccg gatccgaacc gaaccgcggc aatgacgcgg tcggccgctc
     5281 tggacggctc gcgcgcaatc aagggacagg cgcgtgcagc cgatccagct gactgcgcag
     5341 gtcgtcgagc gtgtgcgccg gcagttgaca ggtgccatgg cggcaaaggt aagcctgtcc
     5401 ggcgacgcga tctgcgagga gcggcaccga tcccggcgcg gcattgacga tcgtgaccag
     5461 cggcgcgaac gcggcacgcg ccgcaccgag cgcgccggcg ggtgcgggca gcgcaagttc
     5521 tccgccgcgt tggttcagca gatgcacctg cagtaacgcg ggcgcggccg acgggtgctc
     5581 cagcagcgcg ccgtgtcgtt cgagcgtctg ctgcacgaga tcggcccagt cgccacgctc
     5641 gtacacgccg gccagccgca gcagcagttc gcacgcggct gcggtgccgg acggcaacgc
     5701 gccgtcgaac agatcgcgtg cgcgcaccgg caggtgccct ggctcgccgt cgtagaagcc
     5761 ctcgtccgcg acgtgaaagc gctcgatcat ctgcgccgcg aggtcgcgcg ccgcgtgcag
     5821 ccaatcgacc tcgccggtcg ccgcgtgcaa ctcgaccagg cccagtccga ccagcgcgca
     5881 gtccgacaga aaactctcgg cgcgcagcgt accggcgcgc cacgcatggc gcagtctgcc
     5941 gtccccgcac atcgtgtcga gcaggaagcg cgcgagcgca actgcggtct gcaccaggtc
     6001 ggcgcgctcg agcagccggc ccgcctcggc gaatgcgcgc agcgccatgc cgttccagtc
     6061 ggtgagcacc ttgtcgtcgg tgatcggcca gacgcgccgc gcgcgcaccg cgtacagccg
     6121 atcgcgcacc gaacgctccc accgcgcgaa ctcgtcgggc gtgagaccga gcgccgcgcg
     6181 cgccggctcg ggatcgcgac gctcgagcac gttgacgccg tgttcccagt tgccctcggc
     6241 ggtcgcgccg tacagcctca gtgccgcgtc ggcgtcggcg cccagcaggg cccgcagttc
     6301 ggccgcggtc cacacgtaga acttgccctc gacgccctcg gagtccgcgt cctgcgcgga
     6361 actgaacgcc gcgcgtgccg cggccggatg catttcgcgc cgcaggtacg cgagcgtgtc
     6421 ctcggcgacg aaccgcaacc gcgcgtcgcc ggtcaggcga tacgcgcccg cgtaaacgcg
     6481 cgccagttgc gcgttgtcgt acagcatctt ctcgaagtgc ggcacgtgcc agcgttcatc
     6541 ggtcgagtag cgcgcgaagc cgccgccgac ctggtcgtac atgccgccgg cggccatgtg
     6601 cccgagcgtg aacgtcagca ttgcgcgcgc ccgctcgctg ccgatcgcgg agagcgtcag
     6661 caggtactgc agcagcggcg gctgcggaaa cttcggtgcg ccgccgaaac cgccgtgcgc
     6721 gctgtcgaag gtcttcgcca gcgtctcgac cgcgcgttgg tgcgggtccg cgggcggcgc
     6781 gcggcgcggc aactcgatcc cccggcgcac atggtcgagc agcgcgccgg cctgctgttc
     6841 gagttcggag cggcgcgcgc cccacgcctg ctgcaccgcg cgcagcacgt cggcaaagct
     6901 cggcatcccg tatcgcggca gcggcgggaa gtaggtgccg ccgaagaacg gtgccagctg
     6961 cggcgtcagg aacatgttca tcggccagcc accatgttgc gtcagcgcct gcagcgcggt
     7021 catgtacaca tgatcgacgt cgggcagttc ctcgcggtcg accttgatcg gcacgaatcc
     7081 ttcgttcaga agttgcgcga tcgattcgtc ctcgaagctc tcgcgctcca tcacgtggca
     7141 ccagtggcag gtcgcgtagc cgactgacag gaagatcggc ctgtcctcgg cgcgcgcctt
     7201 ggcaaacgcc tcctcgcccc acgggtacca gtcgaccggg ttgtgcgcat gctgcagcag
     7261 gtaaggactg gtttcgcggg ccagccggtt gggtgggtgc gatgcgtggg tcatcgt
//
LOCUS       DBNHBY010000538         7750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_102397, whole genome shotgun sequence.
ACCESSION   DBNHBY010000538 DBNHBY010000000
VERSION     DBNHBY010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_102397"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="AC16HB_13155"
     CDS             complement(<1..280)
                     /locus_tag="AC16HB_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745558.1"
                     /note="transports peptides consisting of two or three
                     amino acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HIR5607780.1"
                     /translation="MLRFILTRLSLVIPTFVGMTLVAFLMIRLVPGDPIETLAGERGI
                     DPARHAALVKEYGLDQPVLTQYAIYIGRVLRGDLGKSMITQEPVVHEFL"
     gene            767..1156
                     /locus_tag="AC16HB_13160"
     CDS             767..1156
                     /locus_tag="AC16HB_13160"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR5607781.1"
                     /translation="MSRRISIVVVSGSHERLQMAAMLASVGAVTGNDVRVFLSMNALP
                     PFLKGTPSAAPAEGEVGELLARKNVPPFKQLFENAVDLGSAQLLPCSMAMDLLGYEPD
                     QLESYLGAPTGLTKFLNDAEEGQLLSF"
     gene            1189..1416
                     /locus_tag="AC16HB_13165"
     CDS             1189..1416
                     /locus_tag="AC16HB_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5607782.1"
                     /translation="MSRKIIDARGSFCPGPLMELIAAMKMVEVGDEIEVLSSDKGSST
                     EIPEWIGKVKHEYLGTEQRDGVWHVLVKKTH"
     gene            1435..2568
                     /locus_tag="AC16HB_13170"
     CDS             1435..2568
                     /locus_tag="AC16HB_13170"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5607783.1"
                     /translation="MRILIVGGGMGGTILANNLARRLTDVMRSGKVQLTMLSASDRHM
                     YQPGLLYLAVGRMTPDELYRDQASLLEPGIEFLVDPVEEFRLDKNEVKTKSGKVIGYD
                     VMAIATGSRPTPEEIPGLAENAEQFYTEESAVKMFKRLREFKGGKVVIAMGVPHKCPM
                     APLEILFMLHDFFKDRGVLDKVKLHYTYPIGRVHTLENVAKWAAPEMDRLGITYETLF
                     NLKEVDGGKKIVRSEEGTETDYDLLITIPAHRGMEVIEKNGLGQRGWIPTDRYRLNME
                     GRDNVFVLGDTTNLPISKAGSTAHFEAETLGENIAAMVKLGVPVRDYDGKVFCFIEAG
                     KDRATYAMFDYQNPPQPRPPTKAVHWFKLAYNKLYWASARGLL"
     gene            2586..3194
                     /locus_tag="AC16HB_13175"
     CDS             2586..3194
                     /locus_tag="AC16HB_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607784.1"
                     /translation="MADTPDKDLLSFEGERLIAAARDSLTDEMVTRLAGTATDAMDLI
                     DRVNRTGLYKAIPAIAEMVNNGDLDRLARLARLYSSAEDALTDDMVNRLAETAAEGLS
                     LLDRLNRGGAGRMVEMLARLEASGSLKRIADTLPKLLERLELLDSLLHAIEGAGAVTA
                     QAPKSGGGVGGLWSVLREPESQDALRYLIDVGKAVRSATARR"
     gene            complement(3220..3783)
                     /gene="dcd"
                     /locus_tag="AC16HB_13180"
     CDS             complement(3220..3783)
                     /gene="dcd"
                     /locus_tag="AC16HB_13180"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR5607785.1"
                     /translation="MTIKSDKWIRRMAAQGMIEPFEPGQVRTANGHKIVSYGTSSYGY
                     DVRVAREFKIFTNINSTIVDPKAFDANSFVDFKGDVCIIPPNSFALGHTVEYFRIPRN
                     VLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYAGEGCAQVIFIE
                     SDEVCETSYKDRGGKYQGQTGVTLPKT"
     gene            3913..6315
                     /locus_tag="AC16HB_13185"
     CDS             3913..6315
                     /locus_tag="AC16HB_13185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HIR5607786.1"
                     /translation="MTTALPVDKLLRKADLSAAAFESTAELVPLDGLLGQSRAADALQ
                     LGTRIDAPGFNLFVIGSAGASIDRAVKNLLVDKAAARPVPSDWVYVNDFADPRTPNAI
                     ELPPGRGVELRHAVKDLIDDLGVALPAAFESQDYQARRGAIDEKFQRRQEEALNALRQ
                     EGIAHGVAVARTPFGFALAPMKDGAVMKPEQINALEESERTALQERMAEFEKKLETLL
                     QTIPRWDKERRDQVRALDRETARYAVGHAIDELKARFADLPRVLEHLDAVHADLLSNV
                     GAFIVQAARREAGDESEALFAGALERYAINVLVTQPADAKGAPVVEELHPTIGNLLGR
                     VEHESQQGVLITNFRHIKAGALHRANGGYLILDALAVLSEYYSWMALKRVLKSGRIRI
                     ESLGDLLSLTSTVSLEPEPIPLDVKIVLYGDRILYYLLAMYDPELTKHFKVVADFEDE
                     LSRSPEGEAMFARLIAGIAQGAQLKPLDRAAVARVIEEAARLAEDAHKLTLRTDAIRD
                     LLTEADFLAGEAQRAQISATDIDRAARERVRRAARLSERVRESITRDIALIDTAGARA
                     GQINGLSVFELGGFLFGRPTRITARVRPGAGRVVDIEREVKLGGPIHSKGVLILAGFI
                     SGRYALDVPMSLAASIVFEQSYGGVEGDSASCAELATLLSALADAPLRQDLAITGSIN
                     QLGQVQAIGGVNAKIEGFFDVCNARGLTGTQGVLVPAANVQHLMLRADVIDACAAGRF
                     AVYAIADIDEALALLTGMPAGTRDAEGQFPPDSVNGRVEAQLRAFARVQQAKPGPNQE
                     PV"
     gene            6323..7048
                     /locus_tag="AC16HB_13190"
     CDS             6323..7048
                     /locus_tag="AC16HB_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607787.1"
                     /translation="MAASIRSVVVSLSAAAPSTELLRLAADIARLIGADVRALYVEDA
                     SLFDVADLPLAREFDPLQPGAQRWRPLAREQLARDFELAAAALRRRLADVARTVGVQW
                     EFLVVRPGAQPTPLDQLREQRDFLLLLPAERKPEPPLPAGAVLYAPSGPTRRRGDVIA
                     LAPSADAPAARLADEIARSARERLIVIDAAGHAEQLQHALGRLHERLIVAGARAFADD
                     NDAAQLAALRRVPVLVVPEDAGG"
     gene            7052..>7750
                     /gene="rtcA"
                     /locus_tag="AC16HB_13195"
     CDS             7052..>7750
                     /gene="rtcA"
                     /locus_tag="AC16HB_13195"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR5607788.1"
                     /translation="MTPLRRATAALEIDGSWGEGGGQLIRTAIALAAITSTPIRVRNV
                     RAARRPPGLAPQHLAAVRAVAALCDGTVSGVELRAAQFDFTPRAIRGGEFRFDVGTAG
                     SVTLLAQALLPVLAYAPAASRTTLIGGTDVRAAPPFDYFRHVFCSHLAALGVRARCTL
                     GRRGYYPRGGGEVELDVTPARPRAHRFSGAGAGPIAGCVHTDQLPDHIAARMAEAARA
                     RLPRPARIALEVSAA"
BASE COUNT         1297 a         2566 c         2705 g         1182 t
ORIGIN      
        1 ccaggaactc gtgcaccacc ggctcctggg tgatcatcga cttgcccagg tcgccgcgca
       61 gcacgcggcc gatgtagatc gcatactggg tgagcaccgg ctggtcgagc ccgtactcct
      121 tgaccagtgc cgcgtgacgc gcgggatcga tgccgcgctc gcccgccagc gtctcgatcg
      181 gatcgccggg caccaggcgg atcatgagga acgccaccag ggtcatgccc acgaaggtgg
      241 gaatgaccag cgacaggcgc gtcaggatga agcgcagcat gcacaaattc tatccgcgcg
      301 cctttgcggg acccgcattg gggaacccgc agcgcatccg tcgcggcgtt cccgtcgcgc
      361 cggctgcata cgctgattgc ggaaccgatc cgccggccaa ttcgtcccgg cgcacgattg
      421 agagacgaat tgaacaaccg ccgttgcggt tcacgccaga gacttggatc ggatgccacg
      481 ggcgtcgcgc ggctcgcacc aacgggcttg gcgattgcgc tgcgtaagta gaagtacaaa
      541 cgccattcac cagcagcgag cgagccgtcc ggtgcgcgcg gaaccagcaa gtcctgcgtc
      601 aatgatcgac ctcaagaaga atcgccttct cgcacgctca cacgtgagtc cgcgccgcgc
      661 ggaaattcat gcgcccggac gggcttttca aataagatga atcttgcgtt gaagcgaacg
      721 cggcggtctt gcaccgacgt cgcgcgcttc gggagcctgc acggacatga gccgacgcat
      781 cagcatcgtc gttgtttccg gatcgcacga aaggctgcag atggcggcga tgctcgcatc
      841 ggtcggcgcc gtcaccggaa atgacgtgcg cgtgttcctg tcgatgaatg cgctgccacc
      901 ctttctcaag ggcacaccgt cggccgcgcc tgccgaggga gaggtcggcg aactgctggc
      961 acgcaagaac gtgccgccgt tcaagcaact cttcgagaat gcggtcgatc tcggttccgc
     1021 acagttgctg ccgtgttcga tggcgatgga tctgctcggc tacgagccgg accaattgga
     1081 gtcctacctg ggcgcgccca ccgggctcac caagtttctc aacgacgcgg aagaagggca
     1141 gctgctgtcc ttctgacggg tcgaggtgtt cttgacggag cattcagcgt gagcaggaaa
     1201 atcatcgacg cgcgcggcag cttctgcccc ggccccctga tggaactcat cgcggccatg
     1261 aagatggtcg aggtcggcga cgagatcgaa gtcctgtcga gcgacaaggg ctcgtcgacc
     1321 gagatccccg agtggatcgg caaggtcaag cacgagtacc ttggcaccga acagcgggac
     1381 ggcgtctggc acgtcctggt gaagaagacc cactgagcac gagcggaggc gcacatgcgg
     1441 atactgatcg tcggcggcgg aatgggcggc acgatcctgg ccaacaatct ggcgcgtcgc
     1501 ctgaccgatg tcatgcgcag cggcaaggtg cagctgacga tgctgtcggc gtcggaccgc
     1561 cacatgtacc agcccgggct gctgtatctc gcggtcggcc gcatgacgcc ggacgaactg
     1621 tatcgcgacc aggcgagcct gctcgagccc ggcatcgagt tcctggtcga tccggtcgag
     1681 gaattccgcc tcgacaagaa cgaggtcaag acgaagagcg ggaaggtcat cggttacgac
     1741 gtgatggcca tcgcgaccgg ctcgcgcccg acgccggagg agatcccggg gctggcggaa
     1801 aacgccgagc agttctacac ggaagaaagc gcggtcaaga tgttcaagcg cctgcgcgag
     1861 ttcaagggcg gcaaggtcgt gatcgccatg ggcgtgccgc acaagtgccc gatggccccg
     1921 ctcgagatcc tcttcatgct gcacgatttc ttcaaggacc gcggcgtgct ggacaaggtg
     1981 aagctgcact acacgtaccc gatcggccgc gtgcacacgc tggagaacgt cgccaaatgg
     2041 gccgcgccgg agatggaccg gctcggcatc acgtacgaga cgctgttcaa cctgaaggag
     2101 gtcgacggcg gcaagaagat cgtgcgcagc gaggagggaa ccgagaccga ctacgacctg
     2161 ctgatcacga tcccggccca tcgcggaatg gaggtcatcg agaagaacgg cctcggccag
     2221 cgcggctgga taccgacgga ccgatatcgc ctgaacatgg agggtcgcga caacgtcttc
     2281 gtgctcggag acaccaccaa tctgccgatc agcaaggccg gctcgaccgc gcacttcgag
     2341 gccgagacgc tcggcgagaa catcgccgcg atggtcaagc tcggcgtgcc ggtgcgtgac
     2401 tacgacggca aggtgttctg cttcatcgaa gccggcaagg accgcgccac gtatgcgatg
     2461 ttcgattacc agaatccacc ccagccgcgg ccgcccacca aggccgtgca ctggttcaag
     2521 ctcgcctaca acaagttgta ctgggcgtcc gcgcgcgggc tgctttgagc cgaggggaga
     2581 tcgacatggc ggacacaccg gacaaggatc tgctgtcgtt cgaaggcgaa cgcctgatcg
     2641 cagcagcgcg tgattcactg acggacgaga tggtcacgcg cttggccggc acggccaccg
     2701 atgcgatgga tctgatcgat cgcgtcaacc gcaccggcct ttacaaggcg atacccgcga
     2761 tcgcggagat ggtcaacaac ggtgacctcg accggctcgc gcggctggcg cgactttaca
     2821 gctcggctga agacgcactc accgacgaca tggtgaaccg gctcgccgag acggcggcgg
     2881 agggcttgtc gctgctcgat cggctgaacc gcggcggcgc cgggcggatg gtggagatgc
     2941 tggcgcgcct ggaggcctcc ggcagcctga agcgcattgc cgatacgctg cccaagctgc
     3001 tcgagcgcct cgaactgctg gattcactgc tgcacgcgat cgagggcgcc ggcgccgtca
     3061 ctgcgcaggc cccgaagtcg ggcggcggcg tcggcggcct gtggagcgtg ctgcgcgagc
     3121 ccgaatcgca ggatgccttg cgctacctga tcgacgtcgg caaggcagtg cggtcggcga
     3181 cggcacggcg ctgaggcgcg ccgcgcggcg acgcgcgcct cacgtcttcg gcagcgtcac
     3241 gcccgtctgt ccctggtact tgccgccgcg gtccttgtac gaggtctcgc acacctcgtc
     3301 gctctcgatg aagatcacct gcgcgcagcc ctcgcccgcg tagatcttcg cgggcagcgg
     3361 cgtggtgttg gagaactcca gcgtcacgtg gccttcccac tcgggttcca gcggcgtcac
     3421 gttgacgatg atgccgcagc gcgcgtacgt cgacttcccg aggcagatcg tcagcacatt
     3481 gcgcggaatg cggaagtact cgaccgtgtg gccgagcgcg aacgagttgg gcgggatgat
     3541 gcacacgtcg cccttgaagt cgacgaacga gttcgcgtcg aacgccttcg gatcgacgat
     3601 cgtgctgttg atgttggtga agatcttgaa ctcgcgcgcc acgcgcacgt catagccgta
     3661 gctggaagtg ccgtagctca cgatcttgtg gccgttggcg gtgcgcacct gcccgggttc
     3721 gaacggctcg atcatgccct gcgccgccat gcggcggatc cacttgtcac tcttgatggt
     3781 catctgagat gcaaagcgcg gggtcaaagg gcaacattct aggcccgcgc cggttcattg
     3841 gccgcggtca acgtgggtgc catgcgcggt gatacaagaa acagaaagca acgttcgcgg
     3901 aagaccatcc cgatgactac cgccctgccc gtcgacaaac tgctgcgcaa ggccgacctg
     3961 tcggcggccg cgttcgagtc cactgcggaa ctcgtcccgc tcgacggcct gctggggcag
     4021 agccgcgccg ccgacgcgct gcaactcggc acgcgcatcg atgcgccggg tttcaatctg
     4081 ttcgtgatcg gctcggccgg ggccagcatc gaccgcgcgg tgaagaacct gctcgtggac
     4141 aaggcggcgg cgcgaccggt gccctcggac tgggtctatg tgaacgactt tgccgatccg
     4201 cgcacgccca acgcgatcga gctgccgccc ggtcgcggcg tggaactgcg ccatgcagtg
     4261 aaggacctga tcgacgacct cggcgtggcg ctgccggccg cgttcgagag ccaggactat
     4321 caggcgcgcc gcggcgcgat cgacgagaag ttccagcggc gccaggaaga ggcgctgaac
     4381 gcgctgcgac aggaaggcat cgcccacggc gtcgccgtcg cgcgcacgcc attcggcttc
     4441 gcgctggcgc ccatgaagga cggcgcggtg atgaagccgg aacagatcaa cgcgctggag
     4501 gaaagcgagc gcaccgcgct gcaggagcga atggccgagt tcgagaagaa actcgagacg
     4561 ctgctgcaga cgatcccgcg ctgggacaag gaacggcgcg accaggtgcg cgcgctcgat
     4621 cgcgaaaccg cgcgctacgc cgtcggccat gcgatcgacg agctgaaggc gcgcttcgcc
     4681 gacctgccac gcgtgctcga gcacctggac gcggtgcacg ccgacctgct ctccaatgtc
     4741 ggcgcgttca tcgtgcaggc cgcacggcgc gaggcgggtg acgaatccga ggcgctgttc
     4801 gccggcgcgc tggagcgcta cgcgatcaac gtgctggtga cccagccagc cgatgccaag
     4861 ggcgcgccgg tggtggagga actgcatccg accatcggca acctgctcgg gcgcgtcgag
     4921 cacgagtcgc agcagggcgt gctgatcacg aacttccgcc acatcaaggc gggcgcgctg
     4981 caccgggcca acggcggcta cctgattctc gacgccctcg cggtgctgtc cgagtactac
     5041 agctggatgg cgctcaagcg cgtgctcaag agcggccgca tccgcatcga aagcctgggc
     5101 gacctgctct ccctgaccag caccgtgtcg ctggagcccg agccgatccc gctcgacgtc
     5161 aagatcgtgc tgtacggcga ccgcatcctc tactacctgc tggcgatgta cgacccggag
     5221 ctgacgaagc acttcaaggt cgtggccgac ttcgaggacg aactgtcccg cagtcccgag
     5281 ggcgaagcga tgttcgcgcg cctgatcgcg gggatcgcgc agggcgcgca gctcaagccg
     5341 ctcgaccgcg ccgcagtggc gcgcgtgatc gaggaggcgg cgcgtcttgc cgaggacgcg
     5401 cacaagctga cgctgcgcac cgacgcgatc cgcgacctgc tgaccgaggc cgattttctg
     5461 gccggcgagg cgcagcgcgc gcagatctcg gcgacggata tcgaccgcgc ggcgcgcgag
     5521 cgcgtacgcc gcgccgcgcg cctgtcggaa cgggtgcggg agtcgatcac gcgcgacatc
     5581 gctctgatcg acaccgccgg ggcgcgtgcg gggcagatca acggactgtc ggtgttcgag
     5641 ctgggcggct tcctgttcgg gcggccgacg cgcatcaccg cgcgcgtgcg cccgggcgcg
     5701 ggtcgcgtcg tcgacatcga acgcgaagtc aagctgggcg ggccgatcca ttccaagggc
     5761 gtgctgatcc tggcgggctt catcagcggc cgctacgcgc tcgacgtgcc gatgtcgctc
     5821 gccgccagca tcgtgttcga gcagagctac ggcggggtcg agggcgacag cgcatcgtgc
     5881 gcggaactcg ccacgctgct gtcggcgctg gcggacgcac cgctgcggca ggatctggcg
     5941 atcaccggct cgatcaatca gctcgggcag gtgcaggcga tcggcggcgt gaacgcgaag
     6001 atcgagggct tcttcgacgt ctgcaacgcg cgcggactga ccggcacgca gggcgtgctc
     6061 gtgcctgccg cgaacgtgca gcacctgatg ctgcgcgccg acgtgatcga cgcctgcgct
     6121 gccggcaggt ttgccgtcta cgcgatcgcc gatatcgacg aggcgctggc gctgctgacc
     6181 ggaatgcccg cgggcacgcg cgatgccgag ggacagtttc cgcccgacag cgtgaacggc
     6241 cgcgtcgagg cgcagctgcg cgcgttcgcg cgcgtgcagc aggccaagcc cgggccgaac
     6301 caggaaccgg tgtgagacac cgatggccgc atcgatccgc agcgtggtgg tgagcctgtc
     6361 ggcggcggcg ccgagcacgg agctgctgcg tctcgccgcc gacatcgcgc ggctgatcgg
     6421 cgccgacgtg cgcgcgctgt atgtcgagga cgcgtcgctg ttcgacgttg ccgacctgcc
     6481 gctggcgcgc gagttcgacc cgctgcagcc cggggcgcag cgctggcgtc cgctggcccg
     6541 cgagcagctc gcgcgcgact tcgagctggc ggccgccgcg ctgcggcggc ggctcgccga
     6601 tgtcgcgcgc acggtcgggg tgcagtggga attcctggtg gtgcgtcccg gcgcgcagcc
     6661 gactccgctc gaccagttgc gcgagcagcg cgacttcttg ctcttgttgc cggccgagcg
     6721 caagcccgag ccgccgctgc ctgccggcgc ggtgctgtat gcgccgagcg ggccgacgcg
     6781 ccgccgcggc gacgtgatcg cgctggcgcc cagcgcggac gcgcctgccg cacggcttgc
     6841 cgacgagatt gcgcgcagcg cgcgggaacg attgatcgtg atcgacgccg ccggtcatgc
     6901 cgaacagctg caacacgccc tcgggcgatt gcacgagcgg ctgatcgtcg ccggtgcgcg
     6961 cgcgttcgcc gacgacaacg acgccgcgca actcgccgca cttcgacgcg tgccggtgct
     7021 cgtggtgccg gaggacgcgg gcgggtgaag gatgacccct cttcgccgtg ccacggccgc
     7081 gctggagatc gacggcagct ggggcgaagg cggcgggcag ctgatccgca ccgcaatcgc
     7141 gctggcggcg atcacgtcca caccgatccg cgtgcgcaac gtgcgcgcgg cgcggcgccc
     7201 gccgggcctg gcgccgcagc acctcgcggc cgtgcgcgcg gtcgcggcgc tgtgcgacgg
     7261 cacggtttcc ggcgtggagc tgcgcgccgc gcagttcgac ttcacgccgc gcgcgattcg
     7321 tgggggtgag tttcgtttcg atgtcggcac ggcgggcagc gtgacgctgc tggcgcaggc
     7381 gctgctgccg gtgctcgcgt acgcgccggc cgcgagccgc acgacgctga tcggcggcac
     7441 cgacgtgcgc gcggcgccgc cgttcgacta cttccgccat gtgttctgca gccacctcgc
     7501 ggcgctcggc gtgcgcgcgc gctgcacgct ggggcggcgc ggctactacc cgcgcggtgg
     7561 cggcgaggtc gaactcgatg tcacgccggc gcggccgcgt gcacatcggt tcagcggtgc
     7621 cggcgcgggt ccgatcgcag gctgcgtcca caccgatcaa ctgcccgatc acatcgctgc
     7681 gcgcatggcc gaggccgcgc gcgcgcggtt gccgcggccg gcgcggatcg cgctcgaggt
     7741 ctcggcggcg
//
LOCUS       DBNHBY010000539        13583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1727226, whole genome shotgun sequence.
ACCESSION   DBNHBY010000539 DBNHBY010000000
VERSION     DBNHBY010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1727226"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="ccoG"
                     /locus_tag="AC16HB_13200"
     CDS             complement(<1..1383)
                     /gene="ccoG"
                     /locus_tag="AC16HB_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR5607789.1"
                     /translation="MDSVVEAKRAAQNATASDAGAAPVAKRIEIVESLYQKRKEIYPR
                     ARIGDSLGWFQKWRWATIWATQIVFYGLPWVLWNGRQAVLFDLAARKFYIFGLVLWPQ
                     DVIYLSALLIIAALSLFLFTAVGGRLWCGYACPQTVYTEIFMWIERRIEGDRAKRMAL
                     DKQPLSPRKFLLKSAKHALWIAVAFWTGFTFVGFFTPIRDLGGEILGAQVGGWSAFWI
                     LFYGFATYGNAGWLREQVCLYMCPYARFQSAMFDQDTLIVTYDTARGEPRAHLSRKGG
                     NKQDERRARGEGDCVDCSLCVQVCPTGIDIRKGLQYECIGCGACIDACNYVMQRAGLP
                     RNLIRYTTEHALRDRLTHPQMWSRVLRPRVLVYSGVLLAIVGALVAALVLRVPLKVDV
                     IRDRGAIAREVEDGEVENVYRLQVMNTTESPQTYAISVGGLPGLKLASEREFEVPAAS
                     TRSVVLRLRAP"
     gene            complement(1558..2481)
                     /gene="ccoP"
                     /locus_tag="AC16HB_13205"
     CDS             complement(1558..2481)
                     /gene="ccoP"
                     /locus_tag="AC16HB_13205"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR5607790.1"
                     /translation="MSDFYSVFWDYYVAILTIVSIFACGWLLWSQGRRRVTIVPGQQP
                     DTTGHVWDGDLREFQNPMPRWWIVLFYLTIVFSIVYLILFPGLGTQFKGVLGWSQVKQ
                     HADEVKAADAKYGPIFAAYLAQPLEKVAADARARQMGERIFLNNCAQCHGSDARGSRG
                     FPNLTDSDWLYGGTPDAIEASITGGRQGAMPPMAAAVGGNEDVVDVAHYVLSLSGSAH
                     DSARAARGKEKFAACAACHGPEGKGNQQIGAPNLSDKTWLYGGSVATITETITKGRGG
                     VMPAHKGILSPAEIHVVAAYVYSLSNQPAAK"
     gene            complement(2497..2655)
                     /locus_tag="AC16HB_13210"
     CDS             complement(2497..2655)
                     /locus_tag="AC16HB_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase subunit 3"
                     /protein_id="HIR5607791.1"
                     /translation="MPDINTLRSIVTVLAFVSFIGIVWWAYHRGSRDRFEEAARLPFA
                     DDTQSGQN"
     gene            complement(2666..3280)
                     /gene="ccoO"
                     /locus_tag="AC16HB_13215"
     CDS             complement(2666..3280)
                     /gene="ccoO"
                     /locus_tag="AC16HB_13215"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR5607792.1"
                     /translation="MKLTHDFIERNSTLMIVLIVLVVAVGGLVEIVPLYFQRQTMEPV
                     AGIKPYEPLRLVGRDIYIREGCNNCHSQMIRPFRAETLRYGHYSVAGEFVYDHPFLWG
                     SKRTGPDLARVGGRFSDEWHRIHFNNPRDVVPESNMPGYPWLAKASVEGVDVQARMTA
                     LTRVGVPYNADDIAKAPEMLKGKTELDALIAYLQGLGVQLRNIR"
     gene            complement(3314..4750)
                     /gene="ccoN"
                     /locus_tag="AC16HB_13220"
     CDS             complement(3314..4750)
                     /gene="ccoN"
                     /locus_tag="AC16HB_13220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR5607793.1"
                     /translation="MSQATAAKTYDYGVIRAFSIMTVVWGVVGMLVGVIIAAQMAWPA
                     LNFDIPWLTFGRLRPLHTNAVIFAFGGCGLFATSYYVVQRTSQAPLFAPALAWFTFWG
                     WQAVIVLAAVTLPLGITTSKEYAELEWPIDILIAAVWVSYAIVFFGTIGTRTVKHIYV
                     ANWFFGAFILTVALLHIVNSAEIPVSFWKSYSAYAGAQDAMVQWWYGHNAVGFFLTAG
                     FLGIMYYFVPKAAGRPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSIGMLF
                     SLILLAPSWGGMINGIMTLSGAWHKLREDPILKFLIVSLSFYGMSTFEGPMMSIKTVN
                     ALSHYTDWTIGHVHSGALGWVGLITMGALYWLLPRLYGRTEMYSMRLVTAHFWTATIG
                     IVLYIAAMWIAGVMQGLMWRAVNDDGTLTYTHVESVKATLPFYVIRLSGGLLYLGGMC
                     LMAYNMVRTIAAGRAVAEPVPQLAIAGH"
     gene            complement(4874..5080)
                     /gene="ccoS"
                     /locus_tag="AC16HB_13225"
     CDS             complement(4874..5080)
                     /gene="ccoS"
                     /locus_tag="AC16HB_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HIR5607794.1"
                     /translation="MESLYLLIPLSIVLVLAIGGVLVWSVMSGQFDDLDAEGRRILDE
                     DEAPQRETPVVNYRSHTGHGRPQN"
     gene            complement(5082..7535)
                     /locus_tag="AC16HB_13230"
     CDS             complement(5082..7535)
                     /locus_tag="AC16HB_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5607795.1"
                     /translation="MSAAPLLRKAKELDRAAARDGAREAGACDHCGDSLAGLKVVRRT
                     FVGESRARAYCCLGCAFIAEQLYLARISGRDCAALEAAVLPAAAPRSGAARLQIPVRG
                     MVCAACALLVEHRLRRAPGVVAAQVDFAARRAYVTYDAQRTTPADLAREIARSGYRTG
                     SSEAQEKKAARIELLRLLIAWLAMMQVMMLAVPAYVAGPGEIARDIEQLLRLAQLVLA
                     FPVVVFSGWPIVRAATSQLRAGQIGMDLPIVLGLGAAFGASTWSVLAARGPVYFDSIT
                     MFVALVLGARWLQARLLARAGEHIDAAERRATLTAQRLRAFPASGAVDVVAADELRQG
                     DHVLVAPGETVPADGVVVRGASTLSQAWLTGESTPIDRGEGSVVLAGSVNFDQPLVVE
                     VTRAGSSTSLAALRRLVDDAGRERPRVIETASTVAVIFLWTVIVATALTVFAWLALDP
                     SQALPNAIALLVATCPCALSLAAPAALTATQSALARRGVLTARAAALQPAARVNVAAC
                     DKTGTLTTATPAVARIVLLRERPLHQVVAIAAGLETLSMHPYARAIAAHAAAAKVEPA
                     PVTDARVEGSAGVEARVHGTRYRLGRADFAQALMTAVVERAWSHLPDLLDLHAHAGTG
                     IAVLADGDGPLAVFVFAEAIKRDAAAFAERMRGLGAELILLSGDRREAVERIARELHI
                     ERGLANQTPDSKRQLIAGMQREGRVVAMLGDGMNDAPVLAQADVSIALAEGSTLAQTR
                     ADFIVTSPRLVDAAAVFSAAQRGMAIVRQNLAWALAYNALAIPLAAFGYLTPALAAVG
                     MAASSLLVVGNALRARTID"
     gene            complement(7618..8403)
                     /gene="ugpQ"
                     /locus_tag="AC16HB_13235"
     CDS             complement(7618..8403)
                     /gene="ugpQ"
                     /locus_tag="AC16HB_13235"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5607796.1"
                     /translation="MNDHPAWPYARVLAHRGGGTLAPENTIAAIEVGRAHGFRAVEFD
                     TRLARDDVPVVIHDATLERTTSGSGPVIGRSAAELAQLDAGSWCAPRFAGARVPTLAD
                     VVAYCRAHGIWPNVEIKRVRGDEERVGEVVARAVAVLYRDVVRPDGDRAANVDPRVPL
                     LSSFSRAALAGARRGAPGLARGLLVDDLPADWRDALATLGCVSVHVDHELLTPEQARA
                     IKDAGYWLFCYTVDDPTRARQILDWGVDAFCTDRIDVIGPQFA"
     gene            complement(8400..8864)
                     /locus_tag="AC16HB_13240"
     CDS             complement(8400..8864)
                     /locus_tag="AC16HB_13240"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HIR5607797.1"
                     /translation="MARRVSPRLLRWMLNLWPPYLGAGIRVKRIAADWREVVVEMPLR
                     LRNRNYLGTHFGGSLYSMCDPFYALMVIHNLPGDYLVWDKAASIDFVAPGRSRVSATF
                     RLEETDLAAIRKLTEAGAKHLHVFHTDVVDVEGLAVARVEKIVYVRRKRNGA"
     gene            8925..10148
                     /locus_tag="AC16HB_13245"
     CDS             8925..10148
                     /locus_tag="AC16HB_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5607798.1"
                     /translation="MRKSLGHLRVLDLTRVLAGPWCTQLLADLGADVIKVERPGGGDD
                     TRAWGPPYLKDAHGHDTSEAAYYLSANRGKRSVTIDLSTRAGQELVRTLAKASDVLVE
                     NYRVGQLARYGLDYATLAQVNPRLVYCSITGFGQSGPYKDRAGYDFIIQAMGGFMSIT
                     GERDDRPGGGPQKAGVAVSDLMTGMYAAVAILAALAHRDRTGVGQHIDMALLDTMVAM
                     LANMNMNYLTTGVAPGRAGNAHQNIVPYQVFAAADGHVVIAVGNDAQFTRFCEIAARP
                     DLAADPRFARNADRVRNRNVLVPLLEDVVRGRPMAFWAEQLEAAGIPCGPINDIAHAF
                     ADPQVAHRGMRIAIPHALAGEVPLVASPIKMSATPPAYERPPPRLGEHTDAVLQELVG
                     LSADAVADLRRAGVI"
     gene            10273..11106
                     /locus_tag="AC16HB_13250"
     CDS             10273..11106
                     /locus_tag="AC16HB_13250"
                     /inference="COORDINATES: protein motif:HMM:NF039473.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HIR5607799.1"
                     /translation="MDIAVSRLQQTISGSVLRILRALARVLIRHGLPFDAFVDLAKHA
                     YVDVALSDFSIPGRKPSISRVSVLTGLTRKEVQRMVEAPAQGDGEQQARHNRAARVVA
                     GWVRDADFNVADGPRDLALEERFGFTELVRRYAGDVPPRAVLDELLRVGTVERLPDGR
                     LRLLERVYIPRSSDLGKLTIMGADVAWLIETIDHNLIHGESPRFQRKVMYDNLPVEAL
                     PEFRAYASEHAQHLIELLDHWLSRHDRDANPRVHGSGRMRAGVSIFSFEENLSSNSRG
                     V"
     gene            11108..12562
                     /locus_tag="AC16HB_13255"
     CDS             11108..12562
                     /locus_tag="AC16HB_13255"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR5607800.1"
                     /translation="MEVRAISRVAAALAALALLVRCGGGTDGTGGSTPPAPTAQSVVI
                     GVMTKGSVIVNGIRFDDSTASIRIDNRTGTTNELATGMFVKVLGRINADNISGTADRV
                     EVENEVRGAVQAVNAGGNPPSFTVAGLTIYVDSTTVFANLNPNSIAALTPGVRVEVQG
                     LRDASGNVRASRISGSSNATDELKGVVSNLNATAQTFTLGSTTVNFSAVTPAPTLANG
                     VSVEVHGTFNAGTFSAASIDREDQEDAVFQPSNGGTLDLEGFVSGLNAQTGTFQIGAR
                     VVRTTATTRFEGGSATNLVNGVKVEAEGALDAGGTLVAAKIEFKDASGGSSSSGIVEL
                     TGAVSAVDVGARTLTMFGRTVQINDLTEIDSGLLNNINVGSTRLDVRASLDANGNLIA
                     QQVEATSDTRDAVQARVTARNDAAFTLTLLDTISAALGPNAEYHDASDATITRAQFFA
                     AITPRSASAPGTLVKVRGTFSGGTLNADRAELEN"
     gene            12658..12951
                     /locus_tag="AC16HB_13260"
     CDS             12658..12951
                     /locus_tag="AC16HB_13260"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR5607801.1"
                     /translation="MNATTLYLRIHGRVQGVNFRFSLAAQAGRLGLQGWVRNRRDGSV
                     EAVAQGSAAACDALARWAQDGPPAARVERVEARAASAQELALNATGFATLPTV"
     gene            complement(12948..13544)
                     /locus_tag="AC16HB_13265"
     CDS             complement(12948..13544)
                     /locus_tag="AC16HB_13265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5607802.1"
                     /translation="MTASTGFDTARLRIEVADPRHAPLHPPFFVRNRAHFAPWDPPRS
                     PDIETAAYWEQQLEQAQDEFASGRSARFVALVSEAQTPRLVARANFSQIFRGPFQSCV
                     LGYQIDRDFEGRGLMHEALAACIDYVFGELKLHRIQAAFRLENTRSERLLARLGFERI
                     GVARDYLFIDGAWRDHMLMALTNPRFDPATLSPAPRGG"
BASE COUNT         2300 a         4736 c         4500 g         2047 t
ORIGIN      
        1 cggcgcgcgc agccgcaata ccaccgaacg ggtgctcgcc gccggcactt cgaactcccg
       61 ttcggatgcg agcttcaatc cgggcagtcc gccgaccgag atcgcgtagg tctgcggcga
      121 ctcggtcgtg ttcatcacct gcagacgata gacgttttcg acttcgccat cctcgacctc
      181 gcgcgcgatc gcgccgcgat cgcggatcac gtcgaccttc agcggcacgc gcagcaccag
      241 cgccgcgacc agtgcgccga cgatcgcgag cagcacgccg gagtacacca gtacgcgggg
      301 ccgcagtacg cggctccaca tctgcgggtg cgtgaggcgg tcgcgcagcg cgtgctcggt
      361 cgtatagcgg atcaggttgc gcggcaggcc cgcgcgctgc atgacgtagt tgcaggcgtc
      421 gatgcacgcg ccgcagccga tgcattcgta ctgcagcccc ttgcggatgt cgatgccggt
      481 cgggcagacc tgcacgcaca gactgcagtc gacgcagtcg ccctcgccgc gcgcgcgtcg
      541 ctcgtcctgt ttgttgccgc ccttgcgcga caggtgcgcg cgcggctcgc cgcgcgccgt
      601 gtcgtaggtg acgatcagcg tgtcctggtc gaacatcgcg ctctgaaagc gtgcgtacgg
      661 gcacatgtac aggcacacct gctcgcgcag ccagccggcg ttgccgtagg tcgcaaagcc
      721 gtagaacagg atccagaacg ccgaccagcc gccgacctgc gcgccgagga tctcgccgcc
      781 gagatcacga atcggcgtga agaaaccgac aaaggtgaag ccggtccaga atgccaccgc
      841 gatccagagc gcgtgcttgg cgctcttgag cagaaatttg cgcggcgaca gcggctgctt
      901 gtcgagcgcc atgcgcttgg cgcgatcgcc ctcgatcctg cgctcgatcc acatgaagat
      961 ctcggtgtag acggtctgcg ggcatgcata gccacaccac agtcttccgc ccaccgcggt
     1021 gaacagaaac agcgaaaggg cggcgatgat cagcagcgcc gacaaataga tcacgtcctg
     1081 cggccacaac acgaggccga agatgtagaa cttgcgcgcg gccagatcga acagcaccgc
     1141 ctgccggccg ttccacaaca cccacggcag accgtagaac acgatctgcg tggcccagat
     1201 cgtggcccag cgccacttct ggaaccagcc gagcgagtcg ccgatcctcg cgcgcgggta
     1261 gatctccttg cgcttctggt acagcgactc gacgatttcg atgcgcttgg cgacgggtgc
     1321 cgcacccgcg tcgctcgcag tggcgttctg cgccgcccgt ttggcctcca ctacgctatc
     1381 catgccgttg tgagttgatc cgacgaagaa atgtacgctc caatgccggc cgcctgtgac
     1441 gaccggcgtc acacaccgca cccactgtcg cagaaggcag aacgcccgaa ttggcgcgga
     1501 tcaagcgcgg gcagcgctcg gtgacgccgg tcacgcagac cggccacccc gcggctgcta
     1561 cttggccgcc ggctggttgc tcagcgaata gacgtacgcc gcgaccacat ggatctcggc
     1621 cggcgacagg atgcccttgt gtgccggcat cacgccgccg cgccccttgg tgatcgtttc
     1681 ggtgatggtc gcgacactgc cgccgtacaa ccacgtcttg tcggacaggt tcggcgcgcc
     1741 gatctgctga ttgcccttgc cctcgggccc gtggcacgca gcacaggcgg cgaacttctc
     1801 cttgccgcgc gcggcgcgcg ccgagtcgtg cgcagagccc gacaaggaca gcacgtagtg
     1861 cgcgacgtcg actacgtcct cattgccgcc gaccgcggcc gccatcggcg gcatcgcgcc
     1921 ctggcggccg ccggtgatcg acgcctcgat cgcatccggc gtgccgccgt acagccagtc
     1981 gctgtcagtc aggttcggaa agccgcgcga gccgcgtgca tccgagccat ggcactgcgc
     2041 gcagttgttc aggaagatgc gctcgcccat ctgccgagcg cgcgcgtccg cagcgacctt
     2101 ctcgagcggc tgcgccagat aggcggcgaa gatcggaccg tacttggcgt cggcggcctt
     2161 gacctcgtcg gcgtgctgct tgacctggct ccagccgagc acgcccttga actgcgtgcc
     2221 caaccccgga aacaggatca ggtagacgat cgagaaaacg atcgtcaggt agaagagcac
     2281 gatccaccag cgcggcatcg gattctggaa ctcgcgcagg tcgccgtccc agacgtggcc
     2341 ggtggtatcc ggctgctgcc cgggcacgat cgtcactctg cgccggccct ggctccacag
     2401 cagccagccg caggcgaaga tgctgacgat cgtcaggatc gcgacgtagt aatcccagaa
     2461 gacactgtag aagtcactca tttcgtgatc ccgagttcag ttctgtcccg actgcgtgtc
     2521 gtcagcgaac ggcagccgtg cagcctcttc gaaacggtcg cggctgccgc ggtgatacgc
     2581 ccaccagacg atgccgatga aggagacgaa cgccagcacc gtgacgatcg agcgcagcgt
     2641 gttgatgtcg ggcatggtgg cctccctagc ggatattgcg cagctgcacg ccgaggcctt
     2701 ggaggtaggc gatcagcgca tccagttcgg tcttgccctt cagcatctcc ggcgccttgg
     2761 cgatgtcgtc ggcgttgtat ggcacgccca cgcgcgtcag cgccgtcatc cgtgcctgca
     2821 cgtcgacgcc ttcgacactc gccttcgcca gccacggata gccgggcatg ttcgattcgg
     2881 gaacgacgtc gcgcgggttg ttgaagtgga tgcgatgcca ctcgtccgag aaccggccgc
     2941 cgacgcgcgc cagatccggg ccggtgcgct tgctgcccca aaggaacgga tggtcgtaga
     3001 cgaactcgcc ggccaccgaa tagtggccgt agcgcagcgt ctcggcgcgg aatggccgga
     3061 tcatctgcga gtggcagttg ttgcagccct cgcggatgta gatatcgcgc cccaccagcc
     3121 gcagcggctc gtacggcttg atgcccgcca ccggctccat cgtctgccgc tggaagtaca
     3181 gcggcacgat ctccaccagc ccgccgaccg ccaccacgag cacgatcagc acgatcatca
     3241 gcgtcgagtt gcgttcgatg aaatcatgcg tgagcttcat cttctctctc ttcggtcctg
     3301 tcgcgttctg acgctagtgt ccggcgatcg ccagctgagg caccggttcg gcgaccgcac
     3361 gtccggcggc gatggtcctg accatgttgt aggccatcag gcacatgcca ccgaggtaca
     3421 gcagcccgcc actcaggcga atcacataga acggcaaggt cgccttgacg ctttcgacgt
     3481 gcgtgtaggt gagcgtaccg tcgtcgttga ccgcgcgcca catcaggccc tgcatgacgc
     3541 ccgcgatcca catcgcggcg atgtacagca cgatgccgat ggtggcggtc cagaagtgcg
     3601 ccgtgacgag tcgcatcgag tacatttccg tgcgaccgta cagccgcggc agcagccagt
     3661 agagcgcgcc catcgtgatg aggccgaccc agccgagcgc gcccgagtgc acgtgaccga
     3721 tggtccagtc cgtgtaatgg gacagcgcgt tgacggtctt gatcgacatc atcggcccct
     3781 cgaaggtcga catgccgtag aacgacagcg aaacgatcag gaacttcagg atcggatcct
     3841 cgcgcagctt gtgccacgcg ccgctgagcg tcatgatgcc gttgatcatg ccgccccacg
     3901 acggcgccag caggatcagt gagaacagca tgccgatcga ctgcgtccag tccggcagcg
     3961 cggtgtagtg caggtggtgc ggcccggccc acatgtaggt gaagatcagc gcccagaagt
     4021 gcaccaccga gaggcgatac gaatacaccg gccggccagc ggccttgggt acgaagtagt
     4081 acatgatccc gaggaacccc gcggtcagga agaagcccac cgcgttgtgg ccgtaccacc
     4141 actgcaccat cgcgtcctgg gcaccggcgt aggctgaata ggacttccag aacgacaccg
     4201 ggatctcggc gctgttgacg atgtgcagca gcgccaccgt caggatgaac gcgccgaaga
     4261 accagttggc gacatagatg tgcttgacgg tgcgcgtgcc gatggtgccg aagaacacga
     4321 tggcgtagct gacccagacg gcggcgatca ggatgtcgat cggccactcc agttccgcgt
     4381 attccttgct ggtcgtgatg cccagcggca gcgtcaccgc cgccagcacg atcaccgcct
     4441 gccagcccca gaaagtgaac cacgccagcg cgggcgcgaa cagcggcgcc tgcgacgtgc
     4501 gctgcacgac gtaatagctg gtggcgaaca gtccgcagcc gccgaatgcg aagatcaccg
     4561 cgttggtgtg cagcggacgc agccggccga aggtgagcca ggggatgtcg aagttcagcg
     4621 ccggccacgc catctgcgca gcgatgatca cgcctaccag catgccgacc acgccccaga
     4681 cgacggtcat gatcgaaaaa gcacggatca cgccgtagtc ataggtcttc gccgctgtcg
     4741 cttgactcat ggtcccctct cagatgtgcc gaggcgcgcg tcggacacac cgcttcctcg
     4801 ccggaacagc gcgcgcaatc gctcatgacg cgccgcatcg tattcacgcg agggcgccca
     4861 cggttgacgc aaatcaattc tggggacgac cgtgacccgt gtgagatcgg taattgacaa
     4921 cgggtgtttc ccgctgcgga gcttcgtctt cgtcgaggat gcgccggcct tcggcgtcga
     4981 ggtcatcgaa ctgccccgac atcaccgacc acaccagcac gcccccgatc gcgagcacca
     5041 gcacgatcga cagcgggatc agcaggtaaa gggattccac gtcagtcgat cgtgcgcgcg
     5101 cgcagcgcgt tgccgaccac gagcagcgaa ctcgccgcca tgcccactgc ggccagcgcc
     5161 ggcgtgagat acccgaacgc ggccagcgga atcgccagtg cgttgtatgc gagcgcccag
     5221 gcgaggttct ggcgcacgat cgccatgccg cgctgcgctg cgctgaacac ggcggccgcg
     5281 tcgaccaggc gcggcgacgt gacgatgaaa tccgcgcgcg tctgcgccag cgtgctgccc
     5341 tcggccagcg cgatcgacac gtcggcctgc gccagcaccg gcgcgtcgtt catgccgtct
     5401 cccagcatcg cgaccacgcg tccctcgcgc tgcatgccgg cgatcagctg ccgcttgctg
     5461 tcgggggtct ggttggccaa gccgcgttcg atgtgcagct cgcgcgcgat gcgctcgacc
     5521 gcctcgcgcc ggtcgcccga cagcaggatc agctcggcac ccaggccgcg catgcgctcg
     5581 gcgaacgcgg cagcatcgcg cttgatcgcc tcggcgaaca cgaacaccgc cagcggcccg
     5641 tcgccgtcgg ccaacaccgc gatcccggtg cccgcgtgcg cgtgcagatc aagcagatcc
     5701 ggcaggtgcg accacgcgcg ctcgaccacc gccgtcatca gcgcctgagc gaagtcggcg
     5761 cgcccgaggc gatagcgggt accgtgcacg cgcgcctcga cgccggccga gccctcgacg
     5821 cgtgcgtcgg tgaccggcgc cggctcgacc ttcgcggccg ccgcgtgcgc cgcgatcgcg
     5881 cgcgcgtacg gatgcatcga cagcgtctcc agtccggccg caatggcgac gacctggtgc
     5941 agcggacgct cgcgcagcag cacgatgcgc gcgactgcgg gagtcgccgt ggtcagcgtg
     6001 ccggtcttgt cgcacgccgc cacgttgact cgcgccgcgg gctgcagtgc ggctgcgcgc
     6061 gccgtcaaca cgccgcggcg cgccagcgcc gactgcgtgg ccgtcagcgc cgccggcgcg
     6121 gccagcgaca gcgcgcacgg acaggtcgcc accagcagcg cgatcgcgtt gggcagcgcc
     6181 tgcgacggat cgagcgccag ccacgcaaac accgtgagcg ccgtggccac gatcaccgtc
     6241 cacaggaaga tgaccgcgac cgtgctggcg gtctcgatga cccgtggtcg ttcgcggccg
     6301 gcgtcgtcga ccagccggcg cagcgccgcc agcgatgtgg acgagcccgc gcgcgtcacc
     6361 tccacgacca gcggctggtc gaagttcacg ctgcccgcca gcacgacgct gccctcgcct
     6421 ctatcgatcg gagtcgactc gcccgtcagc caggcctgcg acagcgtgct ggcaccgcgc
     6481 acgaccacgc cgtcggccgg caccgtctcg ccgggagcca ccagcacgtg atcaccttgc
     6541 cgcagttcgt cggccgcgac gacgtcgacc gcgcccgagg cgggaaacgc gcgcagccgc
     6601 tgcgcggtca aggtcgcacg gcgctccgcc gcgtcgatgt gctcgccggc gcgcgcgagc
     6661 agccgtgcct gcagccagcg cgctcccagc accagcgcga cgaacatcgt gatcgaatcg
     6721 aagtacacgg ggccgcgcgc ggcgagcacc gaccacgtgc tggcgccgaa cgccgcgccg
     6781 agtccgagca cgatcggcag gtccatgcct atctgccctg cgcgcagttg actcgtcgcc
     6841 gcgcgcacga tcggccagcc ggagaacacc acgaccggaa acgcgaggac gagctgcgcg
     6901 aggcgcaaca gctgctcgat gtcgcgcgcg atctcgcccg gcccggcgac gtaggccggc
     6961 accgccagca tcatgacctg catcatcgcc agccaggcga tcagcagccg cagcaactcg
     7021 atgcgcgcgg ccttcttctc ctgcgcttcg ctgctgccgg tccgatagcc gctgcgcgcg
     7081 atctcgcgcg cgaggtccgc gggcgtcgtc cgctgcgcgt cgtacgtcac gtaggcgcgg
     7141 cgcgcggcaa agtccacctg cgccgccacg acgcccggcg cgcggcgcag ccgatgttcg
     7201 accagcagcg cgcacgccgc acacaccatg ccgcgcacgg gaatctgcag gcgggcggcc
     7261 ccactgcgcg gcgcggcggc cggcagcacg gccgcctcca gcgctgcgca gtcgcggccc
     7321 gagatccgcg ccagatagag ctgctcggca atgaacgcgc agccgaggca gcagtacgcg
     7381 cgcgcgcgcg actcgccgac gaacgttcgc cgaacgactt tcaacccggc cagggaatcg
     7441 ccgcagtggt cgcacgcgcc cgcctcgcgc gcgccgtcgc gggcagcggc gcgatcgagt
     7501 tccttggctt tgcgaagcag cggggcagcg gacatcgggg gcaggctaag gggagagtta
     7561 cacccgccgt tgatggcggt caaccactca ccgtgcgcgg attggcgatt atcaccgtca
     7621 cgcgaattgc ggaccgatca cgtcgatgcg gtcggtacag aacgcatcca ccccccagtc
     7681 gaggatctgc cgggcgcgcg tcgggtcgtc gaccgtgtag cagaacagcc agtagccggc
     7741 gtccttgatc gcgcgcgcct gctcaggtgt cagcaactcg tgatcgacgt gcaccgacac
     7801 gcagccgagc gtggcgagcg cgtcgcgcca gtcggccggc aggtcgtcga ccagcaggcc
     7861 gcgcgccagg ccgggtgcgc cgcgtcgcgc gcccgcaagc gcggcacgtg agaacgacga
     7921 cagcagcggc acacgcggat cgacgttggc cgcgcgatcg ccatccgggc gcacgacatc
     7981 gcggtacagc acggccaccg cacgcgcgac gacctcgccc acgcgctcct cgtcgccgcg
     8041 cacgcgcttg atctcgacat tcggccagat gccgtgcgcc cggcagtacg ccaccacatc
     8101 ggccagcgtc ggtacgcgcg cgccggcgaa tcgcggtgcg caccaactgc cggcatcgag
     8161 ttgcgccagt tccgccgcgg agcgcccgat caccggcccg ctgccgctgg tggtccgttc
     8221 gagggtggcg tcgtggatta ccaccggcac gtcgtcgcgc gccagccgcg tatcgaactc
     8281 caccgcgcgg aagccgtgcg cacgcccaac ctcgatcgcg gcgatcgtgt tctccggcgc
     8341 cagcgtgccg ccaccgcgat gcgcgagcac ccgtgcgtag ggccaggcag gatggtcgtt
     8401 catgcgccgt tcctcttgcg tcggacgtag acgatcttct cgacccgcgc gacggccagg
     8461 ccttcgacgt cgacgacgtc ggtgtgaaag acatgcaggt gcttggcgcc ggcctcggtc
     8521 agcttgcgga tcgcggccag atcggtctcc tccagtctga acgtcgccga cacccggctc
     8581 cttcccggcg cgacgaaatc gatcgacgcc gccttgtccc agacgaggta atcacccggc
     8641 aggttgtgga tcaccatcag cgcatagaac ggatcgcaca tcgaatacag cgagccgccg
     8701 aagtgcgtgc cgaggtagtt gcggttgcgc aggcgcagcg gcatctcgac cacgacttcg
     8761 cgccagtcgg cggcgatccg cttcacccgg atgcccgcgc ccaggtacgg cggccacagg
     8821 ttgagcatcc agcgcagcag ccgcggagaa actcgtctcg ccatggggcg agtctacaaa
     8881 gcacggcagc gcgcttgata tcctgcgccg ccatgggatc gaatatgcgg aaatcgctcg
     8941 gtcacctccg cgtgctggac ctgacgcgcg tgctggccgg gccgtggtgc acgcaattgc
     9001 tggccgacct cggcgccgac gtgatcaagg tcgagcgccc gggcggcggc gacgacacgc
     9061 gcgcgtgggg gccgccgtac ctgaaggacg cacacggcca cgacacgagc gaggccgcgt
     9121 attacctgtc cgccaaccgc ggcaagcgct cggtgacgat cgacctctcg acccgcgccg
     9181 ggcaggaact cgtgcgcacg ctcgccaagg cgagcgacgt gctggtggag aactaccgcg
     9241 tcggtcagct cgcgcgctac gggctcgact acgcgacgct ggcgcaggtc aatccgcgcc
     9301 tcgtctactg ctcgatcacc ggtttcggcc agagcggtcc gtacaaggat cgcgccggtt
     9361 acgacttcat catccaggcg atgggcggct tcatgtcgat caccggcgaa cgcgacgatc
     9421 gccctggcgg cggcccgcag aaggccggcg tggcggtcag cgacctgatg accggcatgt
     9481 atgcggcggt cgcgatcctg gccgcgctcg cgcatcgtga tcgcacgggc gtcggccagc
     9541 acatcgacat ggcgctgctc gacaccatgg tcgcgatgct tgccaacatg aacatgaact
     9601 acctgaccac cggcgtcgca cccggacgcg ccggcaacgc gcaccagaac atcgtgccgt
     9661 accaggtctt cgccgccgcc gacggccacg tcgtgatcgc ggtcggcaac gacgcgcagt
     9721 tcacgcgctt ctgcgagatc gcagcccgtc cagacctcgc ggccgatccg cgcttcgccc
     9781 gcaacgcgga ccgcgtgcgc aaccgcaacg tgctggtccc gctgctcgaa gacgtggtgc
     9841 gcggcaggcc gatggcgttc tgggccgagc agctcgaggc agccggtatt ccgtgcgggc
     9901 cgatcaacga catcgcgcat gcgttcgcgg acccgcaggt cgcgcaccgc ggcatgcgca
     9961 tcgccatccc gcatgcgctc gcgggcgaag tgccgctggt ggccagcccg atcaagatga
    10021 gcgccacgcc gcccgcctat gagcggccgc cgccgcggct cggcgaacac accgatgccg
    10081 tgctgcaaga attggttggg ctgagtgccg acgccgtggc cgatctgcgc cgcgccggcg
    10141 tgatctgact gccccgctgc ccgcttccgg cgccgtgcgc cggtgatccc atttattcca
    10201 tgattggccg attcttggta gattgcgacc attttctccc acatgaattt tattttggtc
    10261 taactttccc atatggatat cgccgtcagt cgcctccagc agacgatctc ggggtcggtg
    10321 ttgcgcatcc tgcgcgcgct cgcgcgcgtg ctgatccgtc acggtctgcc tttcgatgcc
    10381 ttcgtcgacc tcgccaagca cgcctacgtg gacgtggcgc tgtcggactt ctcgatcccg
    10441 ggccgcaagc cgtcgatctc gcgcgtgtcg gtgctcacgg gcctgacgcg caaggaagtt
    10501 cagcggatgg tcgaggcgcc ggcacagggc gacggcgaac agcaggcgcg ccataaccgc
    10561 gccgcgcgcg tcgtggcggg ctgggtgcgc gacgccgatt tcaacgttgc cgacgggccg
    10621 cgcgatctgg cgctcgagga acgcttcggc ttcaccgagc tcgtgcgccg ctacgccggc
    10681 gacgtgccgc cgcgcgcggt gctcgacgaa ctactgcgcg tcggcaccgt cgagcgcctg
    10741 cccgatggcc ggctgcgcct gctggagcgc gtctacatcc cgcgctcatc ggatctcggc
    10801 aagctgacca tcatgggtgc ggatgtcgca tggctgatcg aaacgatcga tcacaacctg
    10861 atccacgggg agtcgccgcg cttccagcgc aaggtgatgt acgacaacct gccggtcgag
    10921 gcgctgcccg agttccgcgc ctacgcgagc gagcatgcgc agcacctgat cgagctgctg
    10981 gatcactggc tgtcgcggca cgaccgcgac gccaatccgc gcgtgcacgg gagtggccgc
    11041 atgcgcgccg gcgtgagcat cttcagcttc gaagagaacc tgtcgtcgaa ttcaaggggg
    11101 gtctgacatg gaagtgcgcg cgatatcccg cgtcgccgcg gcgcttgccg cgttggcgct
    11161 gctggtccgc tgcggcggtg gaaccgacgg caccggtggt tccacgccgc cggcgccgac
    11221 agcgcagtcg gtcgtgatcg gcgtgatgac caagggcagc gtgatcgtca atggcatacg
    11281 cttcgacgac agcactgcat cgattcgcat cgacaaccga accgggacga caaacgagct
    11341 ggccaccggc atgttcgtca aggtgctggg ccggatcaac gccgacaata tctccggcac
    11401 ggccgaccgc gtcgaggtcg agaacgaggt gcgcggcgcc gtgcaggcgg tgaacgcggg
    11461 cggcaatcca ccgtcgttca cggtcgcggg tctgacgatc tacgtcgaca gcaccaccgt
    11521 gttcgcgaac ctgaacccga acagcatcgc cgcgctgacg cccggcgtgc gggtcgaggt
    11581 gcaagggctg cgcgacgcgt cgggcaacgt gcgcgcttct cgcatcagcg gcagcagcaa
    11641 tgcgaccgac gagctgaaag gtgtcgtttc gaacctgaac gcgaccgcac agaccttcac
    11701 gctcgggtca acgacggtga acttctcggc ggtgacgccc gccccgacgc tcgcaaacgg
    11761 cgtgtcggtc gaggttcacg gtacgttcaa cgcgggcaca ttcagcgccg cgtcgatcga
    11821 ccgcgaagac caggaggatg cggtgttcca gccgagcaat ggtggcacgc tcgatctgga
    11881 gggattcgtc agcggactga acgcacagac gggcacgttc cagatcggcg cgcgcgtcgt
    11941 gcgaaccacc gcgaccactc ggttcgaggg cggctcggcg acgaatcttg tcaacggtgt
    12001 caaggtcgaa gcggaaggcg cgctcgatgc cggcggcacg ctggtcgctg cgaagatcga
    12061 gttcaaggat gcctcgggcg gatcatcgtc gagcggcatc gtcgaactga ccggcgccgt
    12121 cagtgcggtc gacgtcggcg cacgcacgct gacgatgttc ggcaggacag tgcagatcaa
    12181 cgatctgacc gagatcgaca gcggcctgct caacaatatc aacgtcggca gcacgcggct
    12241 cgatgttcgc gcgtcgctgg atgccaatgg caacctgatc gcgcagcagg tcgaggcgac
    12301 cagcgacacg cgcgatgcag tgcaggcgcg cgtcaccgcc aggaacgatg cggcgttcac
    12361 gctgacgctg ctcgacacca tcagcgcggc actcggcccc aacgccgagt atcacgacgc
    12421 cagtgacgcc acgatcacgc gtgcgcagtt cttcgccgcg atcacgccgc gctccgcaag
    12481 cgcgcccggc acgctggtca aggtgcgcgg gacgttcagc ggcggcacgc tgaacgcgga
    12541 tcgggccgaa ctcgagaact gatccgggtc cgcgcgcgca gaggccgccc gcgggcggcc
    12601 tcttgcattt catcggctgg cgccgcgcac ccgctctgct accgttgccc gaaggcgatg
    12661 aacgcgacga ccctgtatct gcgcattcac ggccgcgtcc agggcgtgaa cttccgtttc
    12721 tcgctggcgg cgcaggccgg gcggctcggg ctgcagggct gggtgcgcaa tcgccgcgac
    12781 ggcagcgtcg aggcagtcgc gcaggggtcg gcggccgcgt gcgacgcgct ggcgcgctgg
    12841 gcgcaggacg gtccgccggc ggcgcgagtg gaacgtgtcg aggcgcgcgc tgcgagcgcg
    12901 caggagctgg cgctgaacgc gaccggattc gcgaccttgc cgacggtcta gccgccgcgc
    12961 ggcgctggcg acaacgtggc cggatcgaaa cgcggattgg tcagtgccat caacatgtgg
    13021 tcgcgccagg cgccgtcgat gaacaggtag tcgcgcgcca cgccgatccg ctcgaacccc
    13081 agccgcgcca gcagccgctc gctgcgcgtg ttctcgagcc ggaacgcagc ctggatccgg
    13141 tgcagtttga gctcgccgaa cacatagtcg atgcacgccg ccagcgcctc gtgcatcaac
    13201 ccgcgcccct cgaaatcgcg gtcgatctga tagccgagca cacacgactg gaacggcccg
    13261 cggaagatct gcgagaaatt agcgcgcgcg acgaggcgcg gcgtctgcgc ctcgctcacg
    13321 agcgcgacga agcgcgcgct gcggccgctg gcgaattcgt cctgcgcctg ctccagttgc
    13381 tgctcccagt aggcggccgt ctctatgtca ggcgacctcg gcgggtccca tggcgcgaag
    13441 tgcgcgcgat tgcgcacgaa gaacggcggg tgcagtggcg cgtggcgcgg gtcggcgacc
    13501 tcgatgcgca gccgcgccgt gtcgaatccg gtcgaggcgg tcatgctttg aattcgcgct
    13561 ccgtgggacc cgtaggcgaa gtc
//
LOCUS       DBNHBY010000540        12021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_846340, whole genome shotgun sequence.
ACCESSION   DBNHBY010000540 DBNHBY010000000
VERSION     DBNHBY010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_846340"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="AC16HB_13270"
     CDS             <1..844
                     /locus_tag="AC16HB_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5607803.1"
                     /translation="FDFYGKLAAEELGEPIVVPPRAAEPNADEIAAMAAKPGFVRALA
                     FYRLGLRVEGHREWNWQLRGMTDRQLLAAAEYARSVNVLDRMISTSDRTRGEFDFTQR
                     FPAPHRAELTQSARAAGLDETWVYGLIRQESRFIPDIRSSAGAQGLMQLMPATARFVA
                     RKVGMSDFAPARITEVDVNLRLGTGYLRMVLDDLDGQPVLATAAYNAGPGRPRLWRST
                     LTRPVEGAIFAETIPFNETRTYVKNVMSNAVYYAALFENKAQSLKARLGTITPKEAGS
                     TDLP"
     gene            1027..1359
                     /locus_tag="AC16HB_13275"
     CDS             1027..1359
                     /locus_tag="AC16HB_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HIR5607804.1"
                     /translation="MPATAPATPAYNWAAIDSDPRFQALHTKKVAFLTGLMVFSLIYY
                     FLLPIGAAYFTDIYKIKVYGVVNVGILFALSQFIVAWGIAFYYTAKAKEFDAMAAELV
                     REAEKIGR"
     gene            1363..3264
                     /locus_tag="AC16HB_13280"
     CDS             1363..3264
                     /locus_tag="AC16HB_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute symporter family protein"
                     /protein_id="HIR5607805.1"
                     /translation="MDKRRGLYLAAGALALPLVAMPALAQGAVAPEWRWLTFAVFAAI
                     IGVTMYVTFLAAKRVKTAADFYAAGGGVSGLQNGWAIAGDYLSAASFLGIAGLISLYG
                     YDGFMYSVGWLVAYITVLLVIAEPCRNIGKYTLSDILAYRNDPKKTRVVGALSTITVS
                     TFYLTAQMVGAGVLIKTLIGIDYEVSVITVGVLMLAYVLFGGMVATTWVQIIKAILLV
                     CASILMVILVWSQYGFLGEFLGKVVGDPKIQARVAALLGDKATNLSAAQLGQIFLEPG
                     LFLKVPLDQISLGMALVFGTAGMPHILMRFFTVPTAQAARKSVNWAMAIIGGFYVLTL
                     FLGMASAMKVGPSTIAAIDAGGNMANPLLAQALGGGEHSLLGNLFMAFVAAVAFATIV
                     AVVAGLVLAAASAMAHDLYVGVIREEHVTPQEQVKAARIATIFVGAMAIVIGISAKGQ
                     NVAHLVALAFAVASSANFPCVLLTLFWKRCNTGGIVLGMIVGTIAAIGLVMVSPNLQY
                     PKAIKAAAQKVIDGATAKMAPITEALASGDAAKVAKAEKDKAALDKAVATAKGTIDRL
                     KDDNTSIVGLEEPLFNLRNPGIISIPLGFLAVILGSLLYRDKRAEDLWDEVYVRQTTG
                     ILAAKATAH"
     gene            3354..5264
                     /locus_tag="AC16HB_13285"
     CDS             3354..5264
                     /locus_tag="AC16HB_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HIR5607806.1"
                     /translation="MNELSTPPLIYDSVRAFLARHAPFSSMSEEALRFLIPRLKLAFF
                     PKDAVIVDRQGGPFPPFHIIQRGLVSSVTAGLDTLPDRVLSAGECFPVGALSAGGQPT
                     RTYRAIDDVFAYELDVDSFNKLRQTSPEFEAFCTAAVTVLAQQSLAELQRHYAQVAAD
                     QHSLTRPLSEIVAREPVTCRAETTLTEALTRMRDTSVRTIIVVDPKDAPIGVFTLNDL
                     RDRVVLQQLPLDTPVEQVMTRNPLTLGSDATAAEAMQMMAAGGFHQMIVTERGRVFGT
                     VAEHDLFTLQRVSLRQIHHAIRSARTIAALAHVAGDIRNLAHNLLAQGVSAETLTRTL
                     SALNDALTREVVTRVLQKHDVTNIDWCWMALGSEGRGEQTLSTDQDNALIYARGPALT
                     ASEQLRVRHALLAFAREINEALATLGFPLCKGGIMASNPEWCLAEDEWREQFTRWLSE
                     PTPEALLRANIFFDFRALFGNNTLTDRLSQWLLSRTQNNKLFLRLMVANALQTEPPLG
                     LIRTFVVDDDAEPPGTLDMKVRGSRIFVDAARVFALAQGIGATGTADRLRQAGEKLRV
                     DPRHVDATVEAFHYLQVLRLRTQHRRDAGGSPNRIDPYALNDIDQRMLKETFRQARKL
                     QQRLKETYTFGL"
     gene            5278..5973
                     /locus_tag="AC16HB_13290"
     CDS             5278..5973
                     /locus_tag="AC16HB_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5607807.1"
                     /translation="MSLLANLLHSPPRRSPEIEERLWRLAERTPPPTRNRHSASRYVA
                     VAVETTGPEARRDHLLAIGAVSVDRGRIDLSPCFDVGLGQPESSSRDAILRRGVGGQR
                     QLSGAEAVEALVGFLEFVAHAPLVAFHAEFARSVLDRALKEHLSVRTQSPWIDLARVL
                     PALYPSSESRTMDDWLQLMKIQRIAPHDALAEALATAQMLQVCMPMADSHNLTCPQHL
                     IEVRKAQRRSARR"
     gene            6084..6281
                     /locus_tag="AC16HB_13295"
     CDS             6084..6281
                     /locus_tag="AC16HB_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607808.1"
                     /translation="MGPDIKSQRLVALFLLGSLLFSYPLLYLFNVADTFLGIPLSYLY
                     IFVAWAFLILLMALVAERRDR"
     gene            6322..9111
                     /locus_tag="AC16HB_13300"
     CDS             6322..9111
                     /locus_tag="AC16HB_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5607809.1"
                     /translation="MLADVVIVAASFAYVGLLFAVAYYGDRRADQGRSIIANPYIYSL
                     SLGVYATAWTFYGSVGRAASNGIGFLPIYLGPTLMAALWWVVLRKIIRICKTNRITSL
                     ADFVASRYGKSALLGGLVTIIAVVGIVPYISLQLKAISTTIAILFQYPEIVMPQKLAA
                     VPVLRDLSLYIALLLAVFTLIFGTRHLDLSERHEGLVAAIAFESLMKLVAFLAVGVFV
                     TYWMYDGAADLFAKAAAKPEIRSLFVIGGGGTDVYSASASLASLTFLSMLAVMFLPRQ
                     FQVAVVENVDEQHVKKALWLFPAYMLAFNLFVLPIAFGGLLHFAPGSVDPDTFVLALP
                     MSEHLQWLALLVFIGGLSGATGMVIVETIALATMVCNDLVMPVLLRIRALKVADRRDL
                     TGLLLSIRRGAIVLVLLLGYAYFRLAGEAYALVSIGLISFAAVAQFAPVILGGIFWKG
                     ATRAGALAGLTAGFALWLYTLLLPAFAKSGWLPIGFLEQGPFGLALLKPEALFGLTGL
                     DQITHAMLWSMLFNLGGYIGVSLAGRPSAAVTSQATLFVDALEGAREDVRFWRGTARA
                     TDLHVLLGRFVGQERADEVFADYARRRGLTWPGEGLQADAELVHYVETQLAGTIGAAS
                     ARIMVASAVKEEALSAEEVMNMLDKASQIIVYSHRLEQKSRELERATAELREANERLK
                     SLDKLKDDFVSGVSHELRTPLTSILAFAEILRESPRVSDQERAEFLDIIINESNRLIR
                     LINEILDLSKIEAGRAEWRSTDLNLLEVVKESITATSQLFRDRDVQLDLDLPGTVPLI
                     RADRDRIMQVLINLLSNAVKFCDREHGRVRVRVSAAPGAVRVDVQDNGPGLDARSQEV
                     IFEKFRQAGDVLTDKPQGTGLGLPISRQIVEHFHGRLWVESAPGTGATFSFTLPVGTR
                     APSRDEPRTAATTRA"
     gene            9146..9520
                     /locus_tag="AC16HB_13305"
     CDS             9146..9520
                     /locus_tag="AC16HB_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5607810.1"
                     /translation="MSKKILIADDEANIVRSLQFLMKREGFEVLVAGDGDDALAQIST
                     HRPDLVLLDVMMPRRSGYEVCQLVRANADWNAIKVVLLTAKGRDVDIEKGLAMGADAY
                     ITKPFATKELVAKVKDMLGLAE"
     gene            9541..11697
                     /locus_tag="AC16HB_13310"
     CDS             9541..11697
                     /locus_tag="AC16HB_13310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5607811.1"
                     /translation="MATAAACLLTALGVAAIGVAVWADLGADERAAVAQIMEGRIGLV
                     VLITAAVCGLSGWLVHRLYASRVTRLARLAEEARMMIGANRERRLNADPAPEVAALAR
                     VVNELADERDALQRDVEQKIQDAKASVEEERNRLAALMSELQQSVVVCNLDGLILLYN
                     TRARLQFQALSDTSAGGATGSAIGLGRSIFTILERNMINHALENIQQRLRRGVPQPGA
                     SFLTTTRAGQLLRVRMAPVFTGTARDTDAKGERGIGGYVLMLDNITRDFERETRRDLM
                     LQALTEGSRASLANIRAAVEALHDYPDMEASERDGFVDIVSEEARALSQRLDRTMNEH
                     ADSIKARWPLEEMLGADLIAAAQRRIESRVGIRTATETLDEGLWIKVDSFSLLQGLTY
                     IAHRLHQDFDIREVRFRLAGAGRLAHLDMLWAGTQVALETLHEWELDPMNIGGETSLL
                     TLRDLTERHGGEIWYQRETARQIGFCRLLLPAALPQDVLPASALLHSGSRPDYYDFDL
                     FQQLGRSHALDDQPLAKLTYTVFDTETTGLDPTGGDEIIQIGAVRIVNGRVLKSEYFD
                     QLVDPKRPLSPESTAIHGITEDMLEGRPAIDQVLPAFSAFCADTVLVGHNAAFDMRFL
                     QLKERGTGVAFNQPVLDTLLLSPVIHPNQESHRLEAIAERMGIALMGRHSALGDTMVT
                     AEVFLKMIPLLAQMGIRTLREAREAAERTYYARVKY"
     gene            11909..>12021
                     /locus_tag="AC16HB_13315"
     CDS             11909..>12021
                     /locus_tag="AC16HB_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5607812.1"
                     /translation="MQHKNILVLGGSGFIGRYIINLLVARGCRVLVPSRRR"
BASE COUNT         2017 a         3991 c         3940 g         2073 t
ORIGIN      
        1 cttcgacttc tacggcaagc tcgccgccga ggaactcggc gagccgatcg tcgtgccgcc
       61 acgcgccgcc gagccgaacg cggatgagat cgccgcgatg gccgccaagc cgggcttcgt
      121 gcgcgcgctg gcgttctatc gcctcggtct gcgcgtcgag ggacatcgcg aatggaactg
      181 gcagctgcgc ggcatgaccg atcggcagct gctggccgca gccgagtacg cgcgcagcgt
      241 caacgtgctc gaccggatga tctcgacctc cgaccgtacc cgcggcgagt tcgacttcac
      301 gcaacgcttc cccgcgccgc atcgcgccga gctgacgcag agcgcgcgcg ccgcagggct
      361 cgacgagacc tgggtgtacg ggctgatccg ccaggagtcg cgcttcatcc cagacatccg
      421 ttccagcgcc ggcgcgcagg gcctgatgca gttgatgcct gcgactgcgc gcttcgtcgc
      481 gcgcaaggtc gggatgagcg acttcgcgcc cgcgcgcatc accgaggtcg atgtcaatct
      541 gcgcctgggc accggttacc tgcgcatggt gctcgacgat ctggacggtc agcccgtgct
      601 cgcgaccgcc gcctacaacg cgggccccgg gcggccgcgg ttgtggcgct ccacgctcac
      661 gcgcccggtc gaaggcgcga tcttcgccga gacgatcccg ttcaacgaaa cgcgcaccta
      721 cgtcaagaac gtgatgtcga acgccgtcta ttacgccgcg ctgttcgaga acaaggcgca
      781 gtcgctgaag gcgcggctcg gcaccatcac gcccaaggaa gccgggtcca ccgacctgcc
      841 ctgacgtccg gccaccgcgc aaacgcggga ggcccaacct gacaacacgc ttacgccgcg
      901 gcgcagattg cgcgccgttg gcgagcgcgt aagactgacg caagcttcga tcagtagctt
      961 gccagtcaat tcggaagccg ccgcagagcg gacctcaacg cagcacttca ttctgggaga
     1021 accacgatgc ctgcaaccgc accggcgacg ccggcttaca actgggccgc gatcgattcc
     1081 gatccgcgct ttcaagccct gcacacgaag aaggtggcct ttctcacggg tctcatggtc
     1141 ttctcgctga tctactactt cctgttgccg atcggcgccg cctacttcac cgacatctac
     1201 aagatcaagg tctacggcgt cgtcaacgtc ggcatactgt tcgcgctgtc gcagttcatc
     1261 gtcgcgtggg gcatcgcctt ctactacacg gcgaaggcga aggagttcga cgcgatggcg
     1321 gccgaactgg ttcgcgaagc cgaaaagatc gggaggtagg tcatggacaa gagaagagga
     1381 ctctatcttg cggcgggcgc gctggcgctg ccgctggtgg cgatgccggc gctggcgcag
     1441 ggcgccgtgg cccccgaatg gcgctggctc acgttcgcgg tctttgccgc gatcatcggc
     1501 gtcacgatgt acgtcacgtt tctggccgcc aaacgcgtca agacggcggc cgacttctac
     1561 gccgccggcg gcggcgtctc gggtctgcag aacggctggg cgatcgccgg cgactatctg
     1621 tcggccgcat cgttcctcgg catcgcaggc ctgatctcgc tgtacggcta cgacggcttc
     1681 atgtattccg tcggctggct ggtcgcctac atcaccgttc tgctggtgat cgccgagcct
     1741 tgccgcaaca ttggcaagta cacgctgtcg gacatcctcg cctaccgcaa cgacccgaag
     1801 aagacccgtg tcgtcggcgc gctgtccacg atcaccgtgt cgacgttcta cctgaccgcg
     1861 cagatggtcg gcgccggggt gctgatcaag acgttgatcg gcatcgacta tgaagtgtcg
     1921 gtaatcaccg tgggcgtcct gatgctcgcc tatgtgctct tcggcggcat ggtggccacc
     1981 acctgggtgc agatcatcaa ggccattctg ctggtgtgcg cctcgatcct gatggtgatc
     2041 ctggtctgga gccagtacgg cttcctcggc gagttcctcg ggaaggtcgt tggcgatccg
     2101 aagatccagg cgcgcgtagc cgcgctgctc ggcgacaagg cgaccaacct ttcggcggcg
     2161 cagctcggcc agatcttcct ggagcccgga ttgttcctga aggtgccgct cgatcagatc
     2221 tcgcttggca tggccctggt gttcggcacc gccggcatgc cgcacatcct gatgcggttc
     2281 ttcaccgtgc ccaccgcgca ggcggcgcgc aagtcggtga actgggcgat ggccatcatc
     2341 ggcggcttct acgtgttgac gctgttcctt ggcatggcct cggcgatgaa agtgggcccg
     2401 tcgaccatcg ccgccatcga cgccggcggc aacatggcga acccgctgct ggcacaggca
     2461 ctgggaggtg gcgaacattc gctgctcggc aacctgttca tggcctttgt cgccgccgtc
     2521 gcgttcgcga cgattgttgc cgtggtggcc gggctcgttc tggccgcggc ctcggcgatg
     2581 gcgcacgatc tttacgtcgg cgtgatccgc gaagagcacg tgacgccgca ggagcaggtc
     2641 aaggcggcac gcattgccac catcttcgtc ggcgcgatgg cgatcgtcat cggcatttcc
     2701 gccaagggcc agaacgtcgc acacctcgtg gcgctggcat tcgcggtcgc atcttcggcc
     2761 aacttcccct gtgtactgct gacgctgttc tggaagcgct gcaacacggg cggcatcgtg
     2821 ctcggcatga tcgtcggcac gatcgccgcg atcgggctgg tgatggtgtc gccgaacctg
     2881 cagtatccga aggccatcaa ggcggccgcg cagaaggtca tcgacggtgc aacggccaag
     2941 atggcgccga tcaccgaggc gcttgcctcg ggcgatgcgg ccaaggtcgc caaggcggag
     3001 aaggacaagg ccgcgctcga caaggcggtc gcgacagcca aggggacgat cgaccggctg
     3061 aaagacgaca acaccagcat cgtcggcctg gaagagccgc tgttcaacct gcggaacccc
     3121 ggaatcatct ccattccgct cggcttcctc gcggtgatcc tcggctcgct gctgtatcgc
     3181 gacaagcgcg ccgaagacct gtgggacgag gtctacgtgc ggcagaccac ggggattctt
     3241 gccgccaagg ccaccgctca ttagcgcggc gagttgatcc cgatcttgta gttcccggtc
     3301 gggcagcggt acaaaacggg gcctcttcgc gaggccccta ttttttgcag ccgatgaacg
     3361 aactctcgac gccgccgctg atctatgact cggtgcgcgc ttttctcgcc cggcatgcgc
     3421 cgttttcgag catgagcgag gaggcgctcc ggtttctgat cccgcggctg aagctcgcgt
     3481 tctttcccaa ggatgcggtc atcgtcgacc ggcagggcgg gccgtttcca ccgttccaca
     3541 tcatccagcg cggactcgtc tcgagcgtca ccgccgggtt ggacacgctg cctgatcgcg
     3601 tgctcagcgc cggcgagtgc ttcccggtcg gcgcgctgag cgccggtggg cagccgacgc
     3661 gcacgtaccg cgcgatcgac gacgtgttcg cctacgagct cgacgtcgac agcttcaaca
     3721 agctgcggca gacctcgccc gagttcgagg cgttctgcac cgccgccgtg acggtgctgg
     3781 cacagcagtc gctggcggaa ctgcagcgcc actacgcaca ggtcgcggcc gaccagcact
     3841 cgcttaccag gccgctgtcg gagatcgtcg cgcgcgagcc ggtcacgtgc cgcgcggaga
     3901 ccacgctcac cgaggcgctg acccgcatgc gcgacacttc agtgagaacg atcatcgtgg
     3961 tcgatccgaa ggatgccccg atcggcgttt tcacgctgaa cgacctgcgc gaccgcgtgg
     4021 tgctgcagca gttgccgctc gacacgccgg tcgagcaggt catgacgcgc aacccgctca
     4081 cgctcggctc cgacgccacc gccgccgagg ccatgcagat gatggccgcc ggcggattcc
     4141 accagatgat cgtgaccgag cgcggccgcg tgttcggcac ggtcgcggag cacgatctgt
     4201 tcacgctgca gcgggtcagc ctgcggcaga tacatcatgc gatccgctct gcccgcacga
     4261 tcgccgcgct ggcgcacgtc gcgggcgaca tccgcaacct cgcgcataac ctgctcgcac
     4321 aaggcgtctc ggccgaaacg ctgacgcgca ccctctcggc cctgaatgac gcgttgacgc
     4381 gtgaggtcgt cacgcgcgtg ctgcagaagc acgatgtgac caacatcgac tggtgctgga
     4441 tggcgctggg cagcgaaggg cgcggcgagc agacgctttc gaccgaccag gacaacgcgc
     4501 tgatctacgc gcgcggtccc gctttgaccg cctcggaaca attgcgcgtc cggcacgcgc
     4561 tgctcgcctt cgcgcgcgaa atcaacgagg cgctcgccac gctcggcttc ccgctctgca
     4621 agggcggcat catggcgagc aatccggagt ggtgcctggc cgaagacgaa tggcgcgagc
     4681 aattcacgcg gtggctgagc gagcccacgc ccgaggccct gctgcgcgcc aacatcttct
     4741 tcgatttccg ggcgctcttc ggcaacaaca cgctgacgga ccggctgtcg cagtggttgc
     4801 tctcgcgcac gcaaaacaac aagctgttcc tgcgcctcat ggtggccaac gcactgcaga
     4861 ccgaaccgcc cctggggctg atccggacct tcgtggtcga cgatgatgcg gagccgccgg
     4921 gcacgctcga catgaaggta cgcggctcgc gcatcttcgt cgacgcggcg cgcgtgtttg
     4981 cgctggcgca ggggattggc gccaccggga cggccgatcg cctgcgccag gcgggcgaaa
     5041 agctgcgcgt cgatccgcgc catgtcgatg cgaccgtcga agcgtttcac tatctgcagg
     5101 tgctgcggct tcgcacccag caccggcgtg acgccggcgg ctcgccgaac cgcatcgatc
     5161 cgtacgcgct gaatgacatc gaccagcgca tgctgaagga gacattccgc caggcccgca
     5221 agctgcagca gcggctgaag gaaacctata cgttcggcct ctgacgttca gccagcgatg
     5281 agcctgcttg ccaacctgct gcattcgccg ccgcgccggt cgccggagat cgaagagcgg
     5341 ctctggcgcc tggcagagcg cacgccacca ccgacgcgca accgccattc ggcctcgcgc
     5401 tacgtggccg tcgcggtcga gacgacgggt ccggaggcgc ggcgcgacca tctgctggcc
     5461 atcggcgcgg tcagcgtgga ccgcggtcgc atcgacctgt caccgtgctt cgacgtcggg
     5521 ctggggcagc cggagtcgtc gagccgcgac gccatcctgc gacgcggggt cggggggcag
     5581 cgacagctct ccggcgccga agcggtcgag gcgttggtgg gctttctcga attcgtggcg
     5641 catgcgccgc tggtcgcgtt tcacgccgag ttcgccagat cggtgctcga ccgggcgctg
     5701 aaggagcatc tgagcgtccg cacacaaagc ccgtggatcg acctcgcccg cgtcctgccg
     5761 gccctgtacc cttcgagcga gagccgcacg atggacgatt ggctgcagct catgaagatt
     5821 cagaggatcg cgccgcacga cgcactggcc gaggcgttgg caaccgcgca gatgctgcag
     5881 gtctgcatgc ccatggccga cagtcacaat ctgacctgtc cgcagcacct gatcgaagtc
     5941 cgcaaggctc agcgccgttc cgcccggcgc tgacccgcag gtcgacccgc aggccgcggc
     6001 gggcgaggcg ccggcgcggc agaaacgacc gcaatcaagg ggatcgaact tagaatcgac
     6061 gccaaccggg agcgagccgc gcgatgggtc cggatatcaa gagccagcga ttggttgccc
     6121 tcttcctgct gggcagcctg ctcttcagct acccgttgct gtatctgttc aacgtcgctg
     6181 acacgtttct cggcatcccg ctgtcatacc tgtacatctt cgtcgcctgg gccttcctga
     6241 tcctgctgat ggccctcgtg gccgagcgcc gggaccgatg actccgggcc agcatccgca
     6301 aggacatccg gagcgacctc gatgctggcg gacgtcgtaa tcgtcgcggc atccttcgcg
     6361 tacgtgggcc tgctctttgc cgtggcctac tacggcgaca ggcgtgccga tcagggccgc
     6421 agcatcatcg ccaatccgta tatctattcg ctgtccctcg gcgtgtatgc aaccgcctgg
     6481 acgttctatg gcagcgtcgg ccgcgccgcg agcaacggga tcggcttcct gccgatctat
     6541 ctgggtccga cgctgatggc ggcgctgtgg tgggtcgtac tgcgcaagat catccgcatc
     6601 tgcaagacga accgcatcac gtcgctggcc gacttcgtcg cgtcccgcta cggcaagagc
     6661 gcgctgctgg gcggcctggt cacgatcatc gcggtcgtcg gcatcgttcc ctacatttcg
     6721 ctgcagctca aggcgatctc gacgaccatc gccatcctgt tccagtaccc cgagatcgtc
     6781 atgccgcaga agctggccgc ggtgccggtc ctgcgcgacc tctctcttta catcgcgctg
     6841 ctgctcgccg tcttcacgct gatcttcggc acgcggcatc tggatctgtc ggaacgccac
     6901 gaagggctgg tcgcggccat tgccttcgaa tcgctcatga agctggtggc ctttctggcc
     6961 gtcggcgtat tcgtcaccta ctggatgtac gacggcgccg ccgacctgtt cgcgaaggcg
     7021 gccgccaagc ctgagatccg ttcgctgttc gtgatcggcg gcggcgggac cgatgtctac
     7081 agcgcctcgg cttcgctcgc ttcgctgacg tttctgtcca tgctggccgt gatgttcctg
     7141 ccgcgccagt tccaggtcgc ggtagtggaa aacgtggacg agcagcacgt gaagaaggca
     7201 ctgtggctgt tccccgcata catgctggcc ttcaacctgt tcgtgctgcc gatcgctttc
     7261 gggggactgc tgcatttcgc gccgggcagc gtcgaccccg acacgttcgt gctggcgttg
     7321 ccgatgtcgg agcacctgca gtggctcgcc ttgctggtat tcatcggcgg cctgtcgggc
     7381 gccacgggga tggtgatcgt cgaaacgatc gcgttggcga cgatggtgtg caacgacctg
     7441 gtcatgccgg tgttgctgcg catccgcgcg ctgaaggtgg cggatcgccg ggatctcaca
     7501 gggctgctgc tctcgatccg ccgcggcgcg atcgtactgg tgctgcttct cggctacgcc
     7561 tacttcaggc tcgccggcga agcctacgca ctcgtctcga tcggcctgat ctcgtttgcc
     7621 gccgtggcgc agttcgcgcc cgtgatcctg ggcggaatct tctggaaggg cgcgacgcgt
     7681 gccggcgccc tggccggact cactgcgggc tttgcgctct ggctgtacac gctgctgttg
     7741 cccgcgttcg ccaagtccgg ctggttgccg atcggcttcc tcgaacaggg cccgttcggg
     7801 ctggcgctgc tgaagccgga agcgctgttc ggactgacgg gtctcgatca gatcacgcac
     7861 gcaatgctat ggagcatgct gttcaacctc ggcggctata tcggcgtctc gctcgccggt
     7921 cggccgagcg ccgcggtgac gagccaggcc acgctgttcg tcgacgcgct ggaaggtgcg
     7981 cgcgaggacg tgcgcttctg gcgcggcacg gcccgcgcca ccgacctgca cgtgctgctc
     8041 ggccgctttg tcggacagga acgggcggac gaggtgttcg ccgactacgc gcgccgacgc
     8101 ggactgacct ggcccggcga agggctgcag gccgacgccg aactcgtcca ttacgtcgag
     8161 acgcagctgg ccggaacgat cggcgcggct tcggcacgca tcatggtggc ctcggcggta
     8221 aaggaagagg cgctgtccgc cgaggaagtg atgaacatgc tcgacaaggc gtcgcagatc
     8281 atcgtctaca gccaccggct agagcagaag tcgcgcgaac tggagcgcgc caccgccgag
     8341 ctgcgggagg ccaacgagcg gctcaagagc ctggacaagc tcaaggacga cttcgtgtcg
     8401 ggcgtttcgc acgagctgcg gaccccactc acgtcgatac tcgcgttcgc cgagatcctg
     8461 cgcgaaagtc cccgggtcag cgatcaggaa cgcgccgagt tcctcgacat catcatcaac
     8521 gaaagcaatc gcctgatccg cctgatcaac gagattctcg atctgtccaa gatcgaggcc
     8581 ggccgcgccg aatggcgcag caccgacctc aacctgctcg aggtggtgaa ggagtcgatc
     8641 acggcgacca gccagttgtt ccgtgatcgc gacgtccaac tcgatctcga ccttccgggc
     8701 acggttccgc tcatccgggc ggaccgggac cggatcatgc aggtgctgat caatctgctc
     8761 tcgaacgcgg tcaagttctg cgaccgggaa cacggtcggg tccgggttcg ggtgagcgcg
     8821 gcgccgggcg cagtgcgcgt cgacgttcag gacaatggcc cgggactgga cgcgcgaagc
     8881 caggaagtca tcttcgagaa gttccgccag gcgggcgacg tcctgacgga caagccgcag
     8941 ggaacgggcc tggggttgcc gatcagccgc cagatcgtcg aacatttcca tggtcggctg
     9001 tgggtggaga gcgccccggg taccggcgcg actttctcgt tcacgttgcc cgtcggtacc
     9061 cgggcaccgt cgcgcgacga gccgcgcacg gcggccacga cacgggcatg agccgaatgc
     9121 gcaggcaggg agaggtcgcg cgggtatgag caagaagatc ctgatcgccg acgatgaagc
     9181 caacatcgtc cggtctctgc aattcctgat gaagcgcgaa ggcttcgagg ttctggtcgc
     9241 cggcgacggc gatgacgcgc tggcgcagat ttcgacgcac cggccggacc tggtgctgct
     9301 cgacgtgatg atgcccagga ggagcggcta cgaggtctgc cagctcgtgc gtgccaacgc
     9361 ggactggaat gcgatcaagg tcgttctcct gaccgccaag gggcgcgacg tggacatcga
     9421 gaagggactc gccatgggcg ccgatgccta catcacgaaa cccttcgcga ccaaggaact
     9481 ggtggccaag gtcaaggaca tgctcggcct ggccgaatga agggaacgcc cagaccgtgg
     9541 ttggcaacgg ccgcggcgtg cctgctcact gcgctcggcg ttgcggcgat cggtgtggcc
     9601 gtctgggccg acctcggcgc cgacgaacgc gcagccgtgg cgcagatcat ggaaggccgg
     9661 atcggactgg tggtcctgat cacggctgcc gtgtgcggcc tctccggttg gctggtccac
     9721 cggttatatg ccagccgcgt cacccgcctc gcccggctcg ccgaggaggc gcgcatgatg
     9781 atcggcgcca atcgcgaacg tcggctcaat gcggacccgg cgcctgaggt cgccgcactg
     9841 gcgcgcgtgg tcaatgagct ggcggacgag cgagacgccc tgcagcgtga cgtcgagcag
     9901 aagatccagg acgccaaggc gtccgtggag gaagagcgca accgcctcgc ggcgctgatg
     9961 tccgagctgc agcagagtgt ggtcgtctgc aacctcgacg gtctgatcct gctgtacaac
    10021 acgcgcgcgc gattgcagtt ccaggccctg agcgacacat cggccggcgg cgcaaccggc
    10081 tcggcgatcg gactcggacg ctcgatcttc acgatcctcg agcgcaacat gatcaaccat
    10141 gcactcgaga acattcagca gcggctgcgg cgcggcgtgc cgcagccggg ggcgagcttt
    10201 ctcaccacga cgcgcgccgg acagctgctg cgagtgcgca tggcgccggt cttcacgggc
    10261 acggcacgcg acaccgatgc caagggcgag cgcggcatcg gcggctacgt gctgatgctc
    10321 gacaacatca cacgcgactt cgagcgggag acccgccgcg atctgatgct gcaggcgctg
    10381 accgagggca gtcgcgcgtc gcttgccaac atccgggcgg cggtcgaagc cctgcacgac
    10441 tatccggaca tggaggcctc cgagcgcgac gggttcgtcg acatcgtcag cgaagaggcg
    10501 cgcgccctga gccagcgact cgatcggacc atgaacgagc acgcggactc gatcaaggca
    10561 cgctggccgc tggaggaaat gctcggcgcg gatctcatcg ccgcggcgca gcggcgcatc
    10621 gagtcgcgcg tcggcatccg taccgcgacc gagacattgg acgaagggct ctggatcaag
    10681 gtggacagct tctcgctgct gcagggtctc acctacatcg cgcatcgact gcatcaggac
    10741 ttcgatatcc gcgaggtgcg cttccgcctc gcgggagcgg gccgactggc ccacctcgac
    10801 atgctctggg cgggcaccca ggtcgcgctg gagactttgc atgaatggga gctggatccg
    10861 atgaacatcg gcggggagac cagcctgctc acgttgcgcg atctgaccga acgccacggc
    10921 ggcgagatct ggtaccagcg cgagacagcg cgacagatcg gcttctgccg gctgttgctg
    10981 ccggcggcct tgccgcagga cgtgttgccc gcgagcgcgc tgctgcacag cggcagccga
    11041 cccgactatt acgacttcga tctgttccaa cagctcggca gaagccacgc gctcgacgat
    11101 cagccgctcg ccaagctcac ttacaccgtg ttcgacaccg agaccaccgg gctcgatccc
    11161 accgggggcg acgagatcat ccagatcggc gccgtacgca tcgtgaacgg acgggtgctg
    11221 aagagcgagt acttcgacca gctcgtcgat ccgaagcggc cgctttcgcc cgagtcgacc
    11281 gcaatccacg gcatcaccga agacatgctg gaagggcgac ccgcgatcga ccaggtgctt
    11341 cccgcattca gcgcgttctg cgcggatacc gtgctcgtcg ggcacaacgc cgccttcgac
    11401 atgcgctttc tccagctcaa ggagcgcggc accggtgtgg ccttcaatca accggtgctg
    11461 gacacgctgc tgttgtcgcc ggtgatccat cccaatcagg agtcacacag gctggaggcg
    11521 atcgccgagc gtatgggaat cgcgctcatg ggccggcata gtgcgctggg cgacacgatg
    11581 gtcacggcgg aggttttcct gaagatgatc ccgctgctcg cgcagatggg catccgcacg
    11641 ctgcgcgaag ccagggaagc cgcggaaaga acgtactacg cgcgggtgaa gtactagcga
    11701 cgggctatgg cccgcgcgtt agcgatcatg atccggctgg atcgcgccac ggcgtgacgc
    11761 catagaaatg cgaagctcgt ccgcgtgacg ggatccggca gctcccgtgt gccgaacgcg
    11821 gtttgacctg ggcgatccga cggcacgagc atcaggcaac tagaatccgc cttcgaccgc
    11881 ctctgttcga gcagcgccac gtagcccaat gcagcacaag aacatcctcg tcctcggcgg
    11941 ttccggtttc atcggccgct acatcatcaa cctgctggtc gcgcgcggct gccgcgtgct
    12001 ggtaccgtcg cgtcgacgcg a
//
LOCUS       DBNHBY010000541         1858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1049800, whole genome shotgun sequence.
ACCESSION   DBNHBY010000541 DBNHBY010000000
VERSION     DBNHBY010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1049800"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="AC16HB_13320"
     CDS             complement(<1..1784)
                     /locus_tag="AC16HB_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR5607813.1"
                     /translation="MPVLVVASRPDRIDFFAPDADQPQSLSRSDFIARQQGAACSVRL
                     RPAAPADPDAMSGRTQFGFRWFIPELLKHRAIWRNVVLASLAIQLLALGTPLITQAII
                     DKVVVHRTESTLIALGIGLAVFLVFSSALTWIRQYLVLHTGMRIDAVLGSSVFSHLIE
                     LPPRYFERRPTGVIAARLHGVEQIREFLASAAVSLVLDLPFLSLCLAFMFMYSLPLTL
                     IVLGILGAIVVASLAVSPVFQARLNQEFLLGARNQAFVTEFVAGIETAKSLQMEPVLQ
                     RRYGEYLASHLQASFRTRQVTNTYSVVAQTLEQAMTVAILIIGAWIVMRPAPDASSVF
                     TIGMLVAFQMFASRVSQPLMRLVGLWQQFQQARLAVRRLGDLMDVPTEPYRIAATRAT
                     RSTRSEPVVEIRSLAFRHADDRPFLYRDLDLHISAGESVGLVGSSGCGKSTLAKLLQG
                     FYRPTQGVIRIEGIDTNHLAANELRACFGVVPQETVLFSGTVLDNLMLASPRASFDEV
                     IAACRMAEIHDFIQQLPQGYQTEIGERGAGLSGGQKQRIAIARGLLKKPKVLIFDEAT
                     SNLDQPTAASIARTINALRGKVAIVFIT"
BASE COUNT          376 a          572 c          608 g          302 t
ORIGIN      
        1 tgcgtgatga agacaatggc caccttgccg cgcagcgcat tgatcgtgcg agcaatgctc
       61 gccgcagtgg gctgatcgag gttgctggtt gcctcgtcga agatcagtac cttcggtttc
      121 ttgaggaggc cgcgcgcgat ggcgatgcgt tgcttctgac cgccggaaag acccgctcca
      181 cgctcgccga tctcggtctg gtagccctgc ggcaactgct ggatgaagtc gtggatttcg
      241 gccatccggc aggccgcgat gacttcgtcg aagctcgcgc gcggactggc gagcatcaag
      301 ttgtcgagca ccgtgccgga gaaaagcacc gtctcctgcg gcacgacccc gaagcaggcc
      361 cgcagttcat tggccgccag gtgattcgtg tcgatgcctt cgatccgaat gacgccctga
      421 gtgggccgat agaacccctg cagcagtttg gcaagcgtgg acttgccgca gcctgaagac
      481 ccgacgagcc cgacgctctc gcccgcactg atgtgcaaat ccaggtcccg gtagaggaac
      541 ggccgatcgt cggcgtgacg aaacgcgagg gaacggatct ccacgacggg ttccgacctt
      601 gtagagcgag tggcgcgcgt agccgcgatt cggtagggct cggtcggcac atccatcagg
      661 tcgcccagcc gtctgaccgc cagccgtgcc tgctggaact gctgccacag gcccaccaat
      721 cgcatcagcg gctggctgac ccggctggca aacatctgga acgcaaccag catgccgatc
      781 gtgaacacgc tggacgcatc cggcgccgga cgcatgacga tccaggcgcc gatgatcagg
      841 atcgccaccg tcatcgcctg ctccagtgtc tgtgcgacga cgctgtatgt gttggtgacc
      901 tgccgcgtcc ggaagctcgc ctgcaggtgc gaggccagat attcgccata gcgccgctgc
      961 agcaccggct ccatctgcag gctcttggcg gtctcgatgc cggcgacgaa ctcggtgacg
     1021 aatgcctggt tgcgtgcacc gagaaggaac tcctggttca gccgtgcctg aaagaccggc
     1081 gaaacggcca gactggcgac gacaatcgcc ccgagaattc caagcacgat aagtgtcagc
     1141 ggcaacgagt acatgaacat gaatgcaagg cagagcgaga ggaacggaag gtcgaggacc
     1201 agcgagacgg ccgcggaagc gagaaactcc cggatctgct cgaccccgtg caggcgagcg
     1261 gcgatcacgc cggtgggtcg acgctcgaag tagcgtggcg gcaactcgat gagatggctg
     1321 aacaccgagc ttccgagaac cgcgtcgatg cgcataccgg tgtgcaacac gaggtactgg
     1381 cgaatccatg tcagggcaga cgagaagacg aggaacaccg cgaggccgat tccaagggcg
     1441 atcagcgtcg attcggtgcg atgaacgacg accttgtcga tgatcgcctg ggtgatcagc
     1501 ggcgtcccca gcgccagcag ttggattgcc agcgaggcca gcaccacgtt gcgccagatg
     1561 gccctgtgtt tcaacagttc ggggatgaac caacgaaaac cgaactgcgt gcgaccggac
     1621 attgcatcgg gatccgctgg cgcggcaggc cgcagccgta ccgagcatgc ggcgccctgc
     1681 tgccgcgcga tgaagtcgga tcggctcagc gactgcggct gatcagcgtc cggagcaaag
     1741 aaatcgatgc gatccggtcg cgaggcgacg accagtaccg gcatttggct tcgtgccggg
     1801 tctgccgcgc tcgcaagcca agcgacgaac cgtcccggag tcgacgtcgg atcgtcga
//
LOCUS       DBNHBY010000542         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_644124, whole genome shotgun sequence.
ACCESSION   DBNHBY010000542 DBNHBY010000000
VERSION     DBNHBY010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_644124"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16HB_13325"
     CDS             complement(<1..557)
                     /locus_tag="AC16HB_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5607814.1"
                     /translation="MPIYNPPSRDQQFVIHEVLGAVDEFKAMPRHHEVDVDIVNHVLE
                     ESGKFCAEILLPINQSGDEEGCTYDPKSHAVRAPGGFREAYDQFRAAHWQGLAAEHEY
                     GGQGLPHLLQIALYEMQYSTNQSWAMYPGLTVGAYECLLVHGSKEQKDLYLPKLASGE
                     WTGTMCLTEAHCGTDLGMLRTKAEP"
     gene            complement(570..1271)
                     /locus_tag="AC16HB_13330"
     CDS             complement(570..1271)
                     /locus_tag="AC16HB_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5607815.1"
                     /translation="MSVTTFLQGLVGGTRSSSARARKGDVTRAAIVETALATARREGL
                     EGLTIGALAEQMRMSKSGVFAHFGAREELQLAVLKEYAVRFVDEVLRPAVKKPHGLPR
                     LQAILGNWLKLLVREVEQGCLMVGGASEYDDRPGPLQDAMVAIIGGWRDELVRAIEAA
                     KDSGDLRGDVDPRQLAFEIYGVMLAFHQSARLLRAGDSYKRANAALAQLLRSSRPARR
                     KPVKPVSPRRSRRGT"
     gene            complement(1383..>2024)
                     /locus_tag="AC16HB_13335"
     CDS             complement(1383..>2024)
                     /locus_tag="AC16HB_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HIR5607816.1"
                     /translation="AQPLAPAIASFIARHPAVKFDVNVADRVVDLVEEGFDLAIRVGA
                     VRSELLVARKLGATELVPCASPAYLAAHGTPRLPADLATHQVMTYAYASTPRVWQLLD
                     ADGQLHEVRVQGPLHANSDELAVGAAAAGLGVAFEPDYVVRPALADGRLVRILPNFRG
                     ATLDIWAVYPSRRHLSAKVRLFVAHLADVFAAGRADTPRVTPAHSMRASRRRV"
BASE COUNT          308 a          697 c          694 g          325 t
ORIGIN      
        1 tgcggctcgg ccttcgtgcg cagcatgccg aggtcggtgc cgcagtgagc ctccgtcagg
       61 cacatcgtgc cagtccactc gccggacgcg agcttgggca ggtaaaggtc cttctgttcc
      121 ttcgaaccgt gcaccagcag gcattcgtac gcgccgaccg tgagccccgg atacatcgcc
      181 cacgactgat tggtcgagta ctgcatctcg tacagcgcga tctgcagcag gtgcggcagt
      241 ccctgcccgc cgtactcgtg ctcggccgcc agtccctgcc agtgcgcggc gcggaactgg
      301 tcatacgctt cgcggaagcc cccgggcgcg cgcactgcgt gcgacttcgg gtcataggtg
      361 cagccctctt cgtcgccgga ctggttgatc ggcagcagta tctcggcgca gaacttgccg
      421 gactcttcga gcacgtggtt gacgatgtcc acgtcgacct cgtggtgccg cggcatcgcc
      481 ttgaattcgt cgaccgcacc gagcacttcg tggatcacga actgctggtc gcgcgaggga
      541 gggttgtaga taggcatggc tgctccttgc tacgtgccgc gtcgtgagcg gcggggagaa
      601 acgggtttga ccggcttgcg gcgcgccggg cgcgaactgc gcagcagttg cgccagcgcc
      661 gcgtttgcgc gcttgtagct gtcgccggcc cgcagaaggc gggcagactg gtggaacgcg
      721 agcatgacgc cgtagatctc gaacgccagt tggcgcggat cgacgtcccc ccgcagatcg
      781 ccgctgtcct tcgcggcctc gatcgcacgc acgagttcgt cgcgccagcc gccgatgatc
      841 gccaccatcg cgtcctgcaa cggcccgggg cggtcgtcgt attcgctggc accgccgacc
      901 atcaggcacc cctgctcgac ctcgcgcacc agcagcttca gccagttgcc gaggatcgcc
      961 tgcagccgcg gcaagccgtg cggcttcttc acggccgggc gcagcacctc gtcgacgaag
     1021 cgcacggcgt attccttcag caccgcgagc tgcagttcct cgcgtgcgcc gaagtgggca
     1081 aacacgccgc tcttgctcat ccgcatctgc tcggcgagcg cgccgatggt cagcccctcg
     1141 agaccctcgc ggcgcgcggt cgccagcgcc gtctcgacga tcgccgcccg cgtgacatcg
     1201 cccttgcgcg cccgcgcgct ggacgagcgc gtgccgccga cgaggccctg caggaaggtg
     1261 gtgacggaca tacgtgcgag acggtcgttc gaagaattgc gaacgatcgt actattttcc
     1321 gcgcggcccc cggcgccgcc cctgccgtga tttagagggt ggggtctatt ggtgcggcgc
     1381 gatcagacgc ggcggcggga cgcgcgcatc gaatgggccg gcgtcactcg cggcgtgtcg
     1441 gctcgtccgg cggcgaacac gtcggccagg tgcgcgacga agagccgcac cttggcggac
     1501 aggtggcgtc ggctcgggta cacggcccag atgtccagcg tcgcgccgcg gaagttggga
     1561 aggatgcgca ccaggcgacc atccgccagt gcgggacgca ccacatagtc gggctcgaac
     1621 gcgacaccca gtcccgcggc agcggcgccc acggcgagtt catcgctgtt ggcatgcagc
     1681 ggaccctgca cgcgcacctc atgcagttgg ccgtccgcgt cgaggagctg ccatacgcgc
     1741 ggggtcgacg cgtacgcgta ggtcatcacc tgatgcgtgg cgagatcggc cgggagcctc
     1801 ggggtgccgt gcgcagcgag gtacgcggga gacgcgcaag ggaccaattc ggtcgccccg
     1861 agcttgcgcg cgaccagcag ttcgctgcgc accgcgccga cgcgaatcgc caagtcgaac
     1921 ccctcctcga ccagatcgac gacccggtcc gcgacgttca cgtcgaactt cacggccgga
     1981 tggcgggcga tgaagctcgc gattgcgggg gcgagcggct gcgc
//
LOCUS       DBNHBY010000543         3493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_712166, whole genome shotgun sequence.
ACCESSION   DBNHBY010000543 DBNHBY010000000
VERSION     DBNHBY010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_712166"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..816)
                     /gene="trhA"
                     /locus_tag="AC16HB_13340"
     CDS             complement(205..816)
                     /gene="trhA"
                     /locus_tag="AC16HB_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR5607817.1"
                     /translation="MYRGERLNSITHLVGAALAIAGAAVLIVHAAVLGDPWRITSFSI
                     FGAMLVIMYTASTLYHSLRPPAKDVMRRFDHSAIFLLIAGTYTPFTLVSLRGALGWTM
                     SALVWAAALHGIWRAWRRKNDRPQSALPYALMGWLGLVAAVPLLQALGTAGMLWLALG
                     GLLYTAGIVFYVNDERWRHAHGVWHLFVLGGSASHFTAVLFFV"
     gene            complement(852..2825)
                     /locus_tag="AC16HB_13345"
     CDS             complement(852..2825)
                     /locus_tag="AC16HB_13345"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR5607818.1"
                     /translation="MALLQLTEEQVRTWTRAQKDEWWLRNVYRGDMPQLTIRSAITGF
                     LLGGVLAATALYIAAKTGITIGVGLTAVILAFALYRLMHGAGLAGDFTILENNCTQSI
                     ATAAGYMVAPMISALAAYMLVTGQIIPWWHMIAWMCVVSIIGVLLAFPLKRRFINDEQ
                     LPFPEGRASGVVLDALYTGAASAGMYKARLLGATAVITGVYQAIISDGWMKLLQFKLL
                     RMDQWLGLKEPWTFHERLDAYYYAAAARANGYIPTILGTDIRQLGLRLTLDAAMLGVG
                     GLMGIAVATSCLLGAFVNFVILAPLMIQAGDIAPRIGPSGAAIPISRAEIVNQWSLWW
                     GVTMMVVGSLVSLFGRPEIFKGLLKRRAREEGEDVLRHIEFPLWISAVGIPLFSVLGA
                     WVTHAFFGVPWLLAFISLPLIFVLSVICTNSMALTSWTPTGSLSKITQFTMGAIDRSN
                     PASNLIPAGMTSEIAANAANLLSDIKPGYMLGGKPRQQAIGHVIGIFAGAIACVPLFF
                     LLFLPPDANGVRSPATMVSEQFAFPAALQWKGVAEIIAKGVSALPYSAIVAMVVAAAS
                     AAAIEIARIVTRGRLGLSAVSVGLGVVLPPEATLAMFVGALVFWLMGRRHRTPGTRGH
                     EFWVEGMEPICAGLISGSALMGIGNAIVNVLMN"
     gene            2998..3333
                     /locus_tag="AC16HB_13350"
     CDS             2998..3333
                     /locus_tag="AC16HB_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607819.1"
                     /translation="MNTNRWIKPARAGALVLSLALAGCVVVPADPYYAGGPVAVAPPA
                     PRAEVIGVAPYPGWVWLGGYWNWTGRRHDWVGGHWEAPRPGQRWVPHQWNQGRDGWHF
                     NRGYWQHGR"
BASE COUNT          627 a         1195 c         1154 g          517 t
ORIGIN      
        1 accgcgccga tcaagggcaa ggtctgcccg agctgtcacg ccgccgcgat gatcaagcgc
       61 gatggctgcg agttctgcac ggcgtgcggg tatacggggc agtgttcgta gcagacacgt
      121 cgatggcgcg cactgcggcg accatcaggt cgcgacgcgc aatcatgcct catcgcttcg
      181 atcacgcccc gaatcgaaac ggcatcagac aaagaacagc accgccgtga agtgactcgc
      241 gctgccgccg agcacgaaca ggtgccagac gccgtgcgcg tggcgccagc gctcgtcgtt
      301 gacgtagaag acgatgccgg cggtgtagag cagaccgcca agcgcaagcc acagcatgcc
      361 ggcggtgccg agtgcctgca gtagcggcac ggcggcgacc aagcccagcc agcccatcag
      421 cgcatacggc agcgccgact ggggccggtc gttcttgcgg cgccaggcgc gccagatgcc
      481 gtgcagggcc gcggcccaga cgagcgcgga catggtccag ccgagcgcgc cgcgcaggct
      541 gaccagcgtg aatggcgtgt aggtcccggc gatcagcagg aagatcgctg agtgatcgaa
      601 gcggcgcatc acgtccttcg ccggcggtcg cagcgagtga tacagcgtcg aggcggtgta
      661 catgatcacc agcatcgcgc cgaagatgct gaagctcgtg atccgccacg gatcgccgag
      721 caccgccgcg tgcacgatca gcacggccgc gccggcgatg gccagtgcgg cacccaccag
      781 gtgcgtgatg ctgttcagac gttcgccgcg atacatgcca taccttcacg aagacggcct
      841 gattgtgcca ttcagttcat cagcacattc acgatcgcat tgccgatccc catcaatgcc
      901 gagcccgaga tcaggccggc gcagatcggc tccatgcctt cgacccagaa ctcgtggccg
      961 cgcgtgccgg gcgtgcggtg gcgccggccc atgagccaga acaccagcgc gccgacgaac
     1021 atcgcaagcg tcgcttccgg cggcagcacg acgccgaggc cgaccgacac ggcggacaaa
     1081 ccgagcctgc cgcgggtcac gatgcgcgcg atctcgatcg cggcggcgct cgctgccgcg
     1141 acgaccatcg ccacgatcgc cgagtaaggc agcgcgctga cgcccttggc gatgatctcc
     1201 gccacacctt tccactgcag cgccgccggg aacgcgaact gctccgacac catggtcgcc
     1261 ggcgaacgca cgccgttcgc gtcgggcggc aggaacagca ggaagaacag cggcacgcag
     1321 gcgatcgcgc cggcgaagat gccgatcacg tgtccgatcg cctgctggcg cggcttgccg
     1381 ccgagcatgt agccgggctt gatgtccgac agcaggttgg cggcgttggc ggcgatctcc
     1441 gaggtcatgc cggcgggaat caggttgctg gcgggattgc tgcggtcgat cgcgcccatg
     1501 gtgaactgcg tgatcttcga cagcgatccg gtcggcgtcc acgaggtcag cgccatcgag
     1561 ttggtgcaga tcaccgacag cacgaagatc agcggcagcg agatgaacgc gagcagccac
     1621 ggcacaccga agaacgcgtg cgtcacccac gcgccgagca cgctgaacag cggtatgccg
     1681 accgcggaga tccacagcgg aaactcgatg tgcctcagca cgtcctcgcc ttcctcccgc
     1741 gcgcgtcgct tcaacagccc cttgaagatc tccggccggc cgaacaggct caccagcgag
     1801 ccgaccacca tcatcgtcac gccccaccac agcgaccact ggttgacgat ctccgcgcgc
     1861 gagatcggta tcgctgcgcc ggagggcccg atgcgtggcg cgatgtcgcc cgcctggatc
     1921 atgagcggcg ccaggatcac gaagttgacg aacgcgccga gcaggcaact cgtcgccacc
     1981 gcgatgccca tcagtccgcc gacaccgagc attgcggcgt cgagcgtcag ccgcagtccg
     2041 agctggcgga tgtcggtgcc gagaatggtc gggatgtagc cgttggcccg ggccgccgcc
     2101 gcgtagtagt aggcgtcgag ccgctcgtgg aaggtccacg gctccttcag tccgagccat
     2161 tgatccatgc gcagcagctt gaactgcagc agtttcatcc agccgtcgct gatgatggcc
     2221 tggtacacgc ccgtgatcac tgccgtggcg ccgagcaggc gcgccttgta catgccggcg
     2281 ctcgccgcgc cggtatacag cgcgtcgagc accacgccgc tggcacgtcc ctcgggaaac
     2341 ggcagctgct cgtcgttgat gaagcgccgc ttcagcggga acgcgagcag cacgccgatg
     2401 atcgacacca cgcacatcca cgcgatcatg tgccaccacg gaatgatctg cccggtgacc
     2461 agcatgtacg cggcgagcgc ggagatcatc ggcgcgacca tgtagccggc cgcggttgcg
     2521 atcgactgcg tgcagttgtt ctccaggatc gtgaagtccc cggccaaccc ggcgccgtgc
     2581 atcagccggt agagcgcgaa agcgaggatc accgccgtca gcccgacgcc gatggtgatc
     2641 ccggtcttgg cggcgatata aagcgcggtg gccgccagca cgccaccgag caggaaaccg
     2701 gtgatcgcgg aacgaatcgt cagctgcggc atatcgccgc gataaacgtt gcgcaaccac
     2761 cactcgtcct tctgcgcccg ggtccaagtg cggacttgtt cttcggtcag ctgcagcagc
     2821 gccatgtgtt gtcgttctcg gatctcggag cgccactatc ttcgcagacc ctcgccggct
     2881 cccgcaccga tgtcgctcgc aatttgaaac actttcgcca cacagcttga cgcgcgcgcg
     2941 tcggcgcggc atgtcgcagc gacgttggtt ctgtcatcac ccacggacgg aggccacgtg
     3001 aacacgaacc gatggatcaa gcccgcgcgc gcaggcgcgc tggtgttgtc gctcgcgctg
     3061 gccggctgcg tcgtcgtgcc ggcagatccc tactacgccg gcgggccggt ggcggtcgcc
     3121 ccgcccgcgc cgcgcgccga ggtcattggt gtcgcgccct atcccggctg ggtctggctg
     3181 ggcggctact ggaactggac cggacgccgc cacgattggg tcggcggcca ctgggaagcg
     3241 ccgcgtcccg gccagcgctg ggtgccgcat cagtggaacc agggtcgcga cggctggcac
     3301 ttcaatcgcg gctactggca acacggccgc tgaacccgcg gcaggtgtgc aagcattgac
     3361 gatccccccg acgcgcgggc aggtgtcggc ggaacggctg atccgatctg cgcagcggca
     3421 cgacaggcca tcgccgcgcg actacactgg gtgcgcgatc ttcagggaga cggtccgatg
     3481 ctcaggctgc cgc
//
LOCUS       DBNHBY010000544         2697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1794502, whole genome shotgun sequence.
ACCESSION   DBNHBY010000544 DBNHBY010000000
VERSION     DBNHBY010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1794502"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC16HB_13355"
     CDS             <1..245
                     /locus_tag="AC16HB_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR5607820.1"
                     /translation="LEPAPIVDRVKSESGSFGYNVAAEQYGDLVAMGVLDPTKVTRLG
                     LQNAASVAGLILTTDCVIGEKREARSRGAAMEEMGA"
     gene            complement(242..613)
                     /locus_tag="AC16HB_13360"
     CDS             complement(242..613)
                     /locus_tag="AC16HB_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607821.1"
                     /translation="MSRRQPLHLRLELLRMRGQIERGEMSAALVELRADARRIGALAA
                     TVSTLGGALTSEKVGWVGPVLDVIGHAGGRPFWAALALPVVQAMRRRPAVALAVTVGA
                     LALVGWWVGHKRSAPSPPRQD"
     gene            complement(610..999)
                     /locus_tag="AC16HB_13365"
     CDS             complement(610..999)
                     /locus_tag="AC16HB_13365"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5607822.1"
                     /translation="MASPLDAVRRLSASSLNLLLTRAQFASVELAQSRAQIIRWVGLA
                     LIGAVLALLALMAFSALIVIVFWGQGGPWMLLALAVLYAVASYLAVLRLQREVREAPP
                     LLAETLDEFAKDRDAILGRAEREGAAQ"
     gene            complement(1009..1320)
                     /locus_tag="AC16HB_13370"
     CDS             complement(1009..1320)
                     /locus_tag="AC16HB_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR5607823.1"
                     /translation="MDTVATINRDKLVADARVVLDDVESLLRQAASASGQQAQELRER
                     AADALGRARVKLQDAQVAVSENAKAAARVTEDWVHAHPWGAIGTAAGVGFLIGLLVAR
                     H"
     gene            complement(1403..2617)
                     /locus_tag="AC16HB_13375"
     CDS             complement(1403..2617)
                     /locus_tag="AC16HB_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5607824.1"
                     /translation="MKRRQWILIGAGSALLAAVFVWAFRPQPVPVEVGSVKRAAFEQT
                     VDEDGKTRVIDRYVVSAPLAGKLARVQFEPGDAVTAGTEVARLTPVAPAMLDARTARE
                     LTERVGVAAAALEQARANVARSEAAAAQANADLERQKQLKRDGFIAASAVEQAELAAR
                     VQAQALAAAHFAVDGAQHDLAQARAALLRAHALEKSGARVERPGTAWPIESPVAGRVL
                     RVLQKSEAVVGVGTPLLEIADPARLEIVVDVLSTEATRIAPGARVHIDAGSGLVLDGR
                     VRYVEPAAFTKVSALGVEEQRVNVVIDLVSPPDTWQRLGDGYRVDVRIVVAARADAVV
                     APVAALFRDNDEWAVFALDGGRAARRGVKLGGRNASHVWIEHGLAPGDKVIVYPSDSV
                     TDGKRVKVVRGE"
BASE COUNT          401 a         1038 c          887 g          371 t
ORIGIN      
        1 ggctggagcc ggcgccgatc gtcgatcgcg tgaagtccga aagcggcagc ttcggctaca
       61 acgtcgccgc cgagcagtac ggcgatctcg tcgcgatggg cgtgctcgac ccgaccaagg
      121 tcacgcggct ggggctgcag aacgctgctt cggtcgccgg gctgatcctg acgaccgact
      181 gcgtgatcgg cgagaaacgc gaggcgcgga gtcgcggcgc ggcgatggag gagatgggcg
      241 cctaatcctg ccgcggcggg ctgggcgcgg accgtttgtg tccgacccac cagccgacaa
      301 gcgcaagcgc gccgaccgtc acggcaagcg cgaccgcggg gcgacggcgc atggcttgca
      361 cgaccggaag tgccagcgcg gcccagaacg gacggccgcc tgcatggccg atcacgtcga
      421 gcaccggtcc gacccagccg accttttcac tcgtcaatgc gcccccgagc gtggacaccg
      481 tcgccgcgag cgcaccgata cggcgcgcat cggcgcgcaa ttcgaccagc gcggcggaca
      541 tttccccgcg ctcgatctgg ccgcgcatgc gcagcagttc gagccgcagg tgcaggggtt
      601 ggcggcggct cattgcgccg cgccctcgcg ttcagcccgg ccgaggatcg cgtcgcggtc
      661 cttggcgaac tcgtccagcg tctcggccag cagcggcggc gcttcgcgca cctcgcgctg
      721 caaccgaagc accgcgaggt agctggccac cgcgtacaac accgcgagcg ccagcagcat
      781 ccacggaccg ccctgccccc agaacacgat cacgatcagt gccgagaacg ccatcagcgc
      841 gagcagcgcc agcaccgcgc cgatcagcgc cagcccgacc cagcggatga tctgggcgcg
      901 cgactgcgcc agctcgaccg acgcgaactg cgcgcgcgtc agcagcaggt tcagcgacga
      961 cgccgacagg cgccgtaccg catcgagcgg cgaagccacg tgcggccgtc agtgacgcgc
     1021 gaccagcagc ccgatcagga agcccacgcc cgcggccgtg ccgatcgcgc cccacggatg
     1081 cgcgtgcacc cagtcctcgg taacccgcgc ggcggccttg gcgttctcgc tgaccgccac
     1141 ctgcgcgtcc tgcagcttca cgcgcgcgcg ccccagcgcg tccgcggccc gctcgcgcag
     1201 ttcctgcgcc tgctggccgc tggcggaggc agcctgacgc agcagcgatt cgacgtcgtc
     1261 gagcaccacg cgcgcatcgg cgacgagctt gtcacggttg atcgttgcga ctgtgtccat
     1321 cgttgtcctc catcaaaggc agatccatcg taggtcgact ccggcgcatt gcgttgcgtt
     1381 tccgcaagcg ccgcgtcgtg gatcattcgc cgcggacaac tttaacccgc ttgccgtccg
     1441 tcacgctgtc acttggatag acgatgacct tgtcgcccgg ggccaggccg tgctcgatcc
     1501 agacatggct ggcgttgcgc ccgccgagct tcacgccgcg ccgcgccgcg cgcccgccgt
     1561 cgagcgcgaa cacggcccac tcgtcgttgt cgcggaacag ggccgcgacc ggcgccacca
     1621 ccgcatccgc gcgcgccgcc accacgatgc gcacgtcgac gcggtagcca tcgccgagcc
     1681 gctgccaggt gtccggcggc gacacgagat cgatcaccac gttcacccgc tgctcctcga
     1741 cgccgagggc ggagaccttg gtgaacgcgg ccggttctac gtagcgcaca cgaccgtcaa
     1801 gcaccaggcc gctgccggca tcgatgtgca cgcgcgcgcc cggcgcgatg cgcgtggcct
     1861 cggtcgagag cacgtcgacc acgatctcca gccgggcggg atcggcgatc tccagcaacg
     1921 gcgtgccgac cccgaccacc gcctcgctct tctgcagcac cctcaatacc cggcccgcga
     1981 cgggagactc gatcggccac gcggtgccgg ggcgttcgac gcgggcgccg cttttctcca
     2041 gtgcatgcgc acgcagcagc gccgcgcgcg cctgcgccag gtcgtgctgc gcgccgtcga
     2101 ccgcgaagtg cgccgcggcc agcgcctgcg cctgcacgcg cgccgcgagt tccgcctgct
     2161 cgaccgcgga tgccgcgatg aagccgtcgc gcttcagctg cttctgccgc tccaggtccg
     2221 cattcgcctg cgccgctgcg gcctcgctgc gcgccacgtt ggcgcgcgcc tgctcgagtg
     2281 ccgccgccgc cacgccgacg cgctcggtca gctcgcgcgc ggtgcgcgcg tcgagcatcg
     2341 ccggcgccac gggcgtcagg cgcgcgacct ccgttcccgc cgtgacggca tcgccgggct
     2401 cgaactgcac acgcgcgagc ttgccggcga gcggcgctga aaccacgtag cgatcgatca
     2461 cgcgcgtctt gccgtcctcg tcgacggtct gctcgaatgc cgcgcgcttg acgctcccga
     2521 cctcgaccgg caccggctgc gggcggaatg cccatacgaa gaccgccgcc agcaatgcgg
     2581 aacccgctcc gatcaggatc cactgtctgc gcttcatccg cgcctcattc ccttgtcttc
     2641 aatacggcca ccagatcgag gtgatcgatc cgctgccgca ccaccagcgc gctcgcg
//
LOCUS       DBNHBY010000545         1677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1525171, whole genome shotgun sequence.
ACCESSION   DBNHBY010000545 DBNHBY010000000
VERSION     DBNHBY010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1525171"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..833)
                     /gene="trmD"
                     /locus_tag="AC16HB_13380"
     CDS             complement(78..833)
                     /gene="trmD"
                     /locus_tag="AC16HB_13380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5607825.1"
                     /translation="MRFDVITLFPQMFAALTDYGITRRAREQGLWRLATWNPRDFTTD
                     AHRTVDDRPFGGGPGMVMLAEPLALAVEAARGAQSAPGQVIAFAPAGRPLTDARVREW
                     SQAGALTLVCGRYEGIDQRFLDACVDEVVSVGDFVMSGGEIAALALIDAVVRQLPGAL
                     KEASAADESFAAGLLDGPHYTRPEVWRGLGVPAVLLSGHHADIAGWRREQGLKATALL
                     RPELIEAARRQNLLSAADERVLHAMSPGREVRN"
     gene            complement(856..1380)
                     /gene="rimM"
                     /locus_tag="AC16HB_13385"
     CDS             complement(856..1380)
                     /gene="rimM"
                     /locus_tag="AC16HB_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5607826.1"
                     /translation="MSQWRPTPPADLIELGAVRGAHGVKGWVRIAPHTADAEVLRATR
                     RWWLQWRGAVQAVEVAGLKRHSGQLLAKWPGCETPEAADALQGATVAVARSEFPPPPP
                     GTYYWADLIGARVVNRAGEELGTVRALSNNGAHDLIEVVDGDAVCLVPMIETYVDAID
                     FGARTIRVDWQREW"
     gene            complement(1385..1645)
                     /gene="rpsP"
                     /locus_tag="AC16HB_13390"
     CDS             complement(1385..1645)
                     /gene="rpsP"
                     /locus_tag="AC16HB_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR5607827.1"
                     /translation="MVVIRLARGGAKKRPFFNIVATDSRNARDGRFIERVGFYNPVAA
                     GDETKFRVAADRLTYWQGVGAQVSPTVARLLKEFSQAPAPAA"
BASE COUNT          268 a          630 c          501 g          278 t
ORIGIN      
        1 ctctccaatg accatcgtgt gacgactgtt gtgcgcggaa ggttccgccg ttgtcgccgc
       61 cccagaggat gggaccttca gttccggacc tcgcggcccg ggctcattgc atgcaacact
      121 ctctcgtctg ccgcgctcag cagattctgc ctgcgcgcgg cttcgatcag ttccggccgc
      181 agtaacgccg tggccttcag cccctgctcg cgccgccagc cggcgatatc cgcatgatgc
      241 ccggacagca gcaccgccgg cacgcccaga ccgcgccaga cctccggtcg cgtgtagtgc
      301 ggtccgtcga gcagtccggc ggcgaacgac tcatccgcgg ccgacgcttc cttcagcgcg
      361 ccgggcaact ggcgcacgac ggcatcgatc agcgccagcg cggcgatctc tccgcccgac
      421 atcacgaagt cgccgaccga caccacctcg tcgacgcacg cgtcgaggaa gcgttgatcg
      481 atgccctcgt acctaccgca caccagcgtc agcgcgccag cctgcgacca ttccctcact
      541 cgcgcgtcgg tcagcggccg gcccgcgggc gcgaatgcga tcacctggcc cggcgcgctc
      601 tgcgctccgc gggccgcctc gaccgccagc gccagcggct cggcgagcat caccatgccg
      661 gggccgccgc cgaacggccg atcgtccacc gtccggtgcg cgtcggtcgt gaaatcgcgc
      721 ggattccacg tcgcgagacg ccacaacccc tgctcccgcg cgcgacgcgt gatgccgtag
      781 tcggtcagcg ccgcgaacat ctgcgggaaa agcgtgatca catcgaatcg cacgcttgct
      841 cccagcccga ccttcctacc attcccgctg ccagtcgacg cggatcgttc ttgcgccgaa
      901 atcgatcgcg tccacgtacg tctcgatcat cggcacgagg caaaccgcgt cgccgtcgac
      961 cacttcgatc agatcatgcg ctccgttgtt cgacagtgca cgcactgttc ccaactcctc
     1021 tcccgcgcgg ttgacgactc gtgcgccgat caggtcggcc cagtagtacg tcccgggcgg
     1081 cggcggcgga aattcactgc gcgccaccgc gactgtcgcg ccctgcaatg catccgcggc
     1141 ctccggtgtc tcacagccgg gccacttggc gagcaactgt cccgagtgcc gcttcagccc
     1201 tgcgacttcg acagcctgca ccgcgccgcg ccactgcagc caccagcgcc gtgtcgcgcg
     1261 tagtacctcc gcatccgcag tgtggggcgc gatgcgcacc catcctttga ctccgtgggc
     1321 accgcgcaca gcgcccagtt cgatcaggtc cgcaggcggt gtcggccgcc attggctcat
     1381 cgagtcacgc ggcaggcgca ggggcctggc tgaattcctt caacagtcgc gcgaccgtcg
     1441 ggctcacttg cgcaccgacg ccctgccaat atgtcagccg atcggcggcg acgcggaatt
     1501 tcgtttcgtc tccagccgcg accgggttgt agaaacccac gcgctcgatg aagcggccat
     1561 cacgcgcgtt gcgcgagtcg gtggcgacga tgttgaagaa cgggcgtttc ttggcaccac
     1621 cccgggccag tcggatgacg accatcggaa atccttaaga atccaagact tgcgtta
//
LOCUS       DBNHBY010000546         3460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1322339, whole genome shotgun sequence.
ACCESSION   DBNHBY010000546 DBNHBY010000000
VERSION     DBNHBY010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1322339"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC16HB_13395"
     CDS             complement(<1..406)
                     /locus_tag="AC16HB_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607828.1"
                     /translation="MLLLVAACGSSSSDCATPQIVEPSGPGRLEAATRLNTIPVAEIT
                     RALQGAGVSIPGVTPLYEVTNYRVSYRTLDVHGKEIVASGLVSVPVKARGATSPLLGY
                     QHGTMFKDAEVPSNNAVAAEPAVVFASLGYLVI"
     gene            557..1603
                     /locus_tag="AC16HB_13400"
     CDS             557..1603
                     /locus_tag="AC16HB_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5607829.1"
                     /translation="MQESRRDFITLGATLAAGVVLNGQARAADASSAAVRQTLTATTD
                     LRLATFALTNDATLRTGVVLNDGRVVDIGAEAKRKSMALAFDAASMLSLIAAGDAALK
                     QVRALAEGAQDRLPKLDEVHLAAPIPVAPRNVYAVGWNYLAHFEEGKALRDPSQKYPE
                     HPVFFSKASHCINGPFAPIPYDPKVSTLIDWEGELAVVIGKKGRNIPEAEAMNYVFGF
                     SVINDTTARDVQQTRHGGQWFKGKSLDGHGPMGPWIVTRGVLNHDNLKLQTRVNGVVK
                     QDANTGQMFFKVPTIIAELSMGLTLEPGDIIATGTPPGIGHAMKPPQYLKPGDIMETE
                     ISGIGVIRNEITAV"
     gene            1726..2181
                     /locus_tag="AC16HB_13405"
     CDS             1726..2181
                     /locus_tag="AC16HB_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607830.1"
                     /translation="MNRLVPHAGLLAAAAISLVLAFAPAIAQQSKTVDGLVINLGIVP
                     AEVALRADGHRDAHPMNPPSGSQHLLVTLDEQKTGKRIGDAEVVVEVTDPRGHVEKKP
                     LLHTQAAGLADYSELFVLGSRGTYSVRVVITRQPGATPVEARFTVHHST"
     gene            2440..2643
                     /locus_tag="AC16HB_13410"
     CDS             2440..2643
                     /locus_tag="AC16HB_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5607831.1"
                     /translation="MAIGTVKWFNDAKGFGFITPEDGGKDLFAHFSEIQAGGFKSLTE
                     GQRVEFVVKQGAKGPQAAEIRAV"
     gene            2824..3078
                     /gene="infA"
                     /locus_tag="AC16HB_13415"
     CDS             2824..3078
                     /gene="infA"
                     /locus_tag="AC16HB_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5607832.1"
                     /translation="MAKEEHLEMNGVVNEVLPDCRFRVTLDNGHQLVAYSAGKMRKHH
                     IRILAGDKVSLELSPYDLSKGRITFRHIETRRPASPQRRR"
     gene            3198..>3460
                     /locus_tag="AC16HB_13420"
     CDS             3198..>3460
                     /locus_tag="AC16HB_13420"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5607833.1"
                     /translation="MQQIHSSIDIDAPASLVWAILADFDAYRRWNPYIRAVLGKPCTG
                     NALRIALQGQGRDAASMSSMLTQVREPRELRWRQRYVAPGIYTT"
BASE COUNT          696 a         1124 c         1036 g          604 t
ORIGIN      
        1 cgatcaccaa gtatccaagc gacgcgaaga cgaccgccgg ttcggccgca accgcattgt
       61 tgctcggcac ctccgcgtcc ttgaacatcg tgccgtgctg gtagccgagc agcggacttg
      121 tcgcacctct cgccttcacc ggcacgctga ccaaacccga tgcgacgatt tccttaccgt
      181 gcacatcgag cgtccggtag gacacccggt aatttgtcac ttcgtacagg ggcgtgactc
      241 ccggaatcga cacgcccgcg ccttgcagcg cacgggtgat ctcggccact ggaattgtgt
      301 tcagccgagt cgcggcttcc agccgtccgg gcccgctcgg ctcgacgatc tgcggcgtcg
      361 cgcaatccga tgaagaactg ccgcaggccg cgaccagcaa cagcattgga agcagcagca
      421 gcgaatacgg cagaccggcg cgacagggac gcatgcgtcg atattacttt gcattcgaac
      481 gcccctttgc gtcggcagcg gcaaacgagc atgctctcgg ccgcttgagc caaaatcgac
      541 aattgaggga gacacgatgc aggaatcgag aagggatttc atcacgctgg gcgccaccct
      601 cgccgccggc gtggttctga acggacaggc tcgtgccgcc gacgcttcca gcgccgcggt
      661 gcggcagacg ctcaccgcga ccaccgacct gcgcctggcg acattcgctc tgaccaacga
      721 cgctacgctg cgcaccggcg tagtgctcaa cgacggtcgt gtcgtggaca tcggcgcgga
      781 ggcgaagagg aagagcatgg cgctcgcctt cgatgcggcg agcatgctgt cgctgatcgc
      841 cgccggcgac gccgcgctga agcaggtgcg cgcactggct gaaggcgccc aggaccgcct
      901 cccgaagctc gatgaggtgc acctcgccgc accaatcccg gtcgcgccgc gcaacgtcta
      961 tgccgtgggc tggaattacc tcgcgcactt cgaggaaggc aaggcactgc gcgatccgag
     1021 ccagaagtac ccggagcacc cggtcttctt ctccaaggcc tcacactgca tcaacggccc
     1081 ctttgcgccg attccctatg acccgaaggt ttcaacactg atcgactggg agggcgaact
     1141 cgccgtcgtc atcggcaaga agggccgcaa catccccgag gccgaggcga tgaactacgt
     1201 gttcggcttc tcggtgatca acgacaccac cgcgcgcgat gtgcagcaga cgcgccacgg
     1261 tggtcagtgg ttcaagggca agagcctcga cggccacggc cccatgggac cgtggatcgt
     1321 cacgcgcggc gtgctgaacc acgacaacct gaagctgcag acgcgggtga acggcgtggt
     1381 caagcaggac gcgaacaccg gccagatgtt cttcaaggtg ccgacgatca tcgccgaact
     1441 gtcgatggga ctgacactcg agccgggcga catcatcgcc accggcaccc cgccgggcat
     1501 cggtcatgcg atgaagccgc cgcaatacct gaagcccggc gacatcatgg aaacggagat
     1561 cagcggcatc ggcgtgatcc gcaacgaaat cacggcggtc tgagccgata cgctgaattc
     1621 gacctcggct tcacgcgtca aggggacggg cgctgttgac ctcagtcaaa cagcccgccg
     1681 aggcggcgcc gagcatgaat ttcgtgcagt ttggaggttg atctcatgaa cagactcgtc
     1741 cctcacgcag gcttactcgc tgcggccgca atttcactgg tattggcgtt tgctccagca
     1801 attgcgcagc aaagcaagac cgtcgacggc ctcgtcatca atctcggcat tgtccccgcc
     1861 gaagtggcgt tgcgtgccga tggacatcgg gatgcgcatc cgatgaaccc gccgtcagga
     1921 tcgcagcatc tgctcgtcac actcgacgaa cagaagaccg ggaagcggat cggcgacgcg
     1981 gaggtcgttg tcgaggtgac ggatccgcgc ggccatgtgg agaagaagcc gctgttgcac
     2041 acgcaggcgg ccggcctcgc cgattacagc gaactgttcg tgctcggttc gcggggcact
     2101 tattccgtcc gggtggtcat cacgcgacaa ccaggtgcga cacccgtcga ggcgcgattc
     2161 accgtacacc attcgacgtg acgacgatcg gagagacgag gagctgaact tcaagtgcca
     2221 gtcgaagaaa atatgcgttc gacatgcgtc gagttaacgt gtacccttcg tttgctttcg
     2281 atagagcttc gtctttcctc aggttttctt cggcctgacg caatccaccg cggcagcgca
     2341 tcctaggttt ccccaggttt cacgtttctt gaccgttagc tcgccgcggg acgttctcgc
     2401 ggccgatgac ttctcattca cctatcaagg aacgagaaaa tggctattgg cactgtcaag
     2461 tggttcaacg acgcaaaagg cttcggattc atcacacccg aagacggcgg caaagacctg
     2521 ttcgcgcact tctccgagat ccaggccggc ggcttcaaat cgctgaccga aggccagcgc
     2581 gttgaattcg tcgtcaagca gggcgcaaaa ggcccgcaag ccgccgaaat ccgggctgtc
     2641 tgacgacggt ctgatcaacg acgcgcggcc cggcgccgcg cctcgtcttc cattgcatcg
     2701 cgcgacccgc gcgacgcaag cacccatcca cgtcgcaaca aggcgcaggc ctcgttgcgc
     2761 ccacgcggga ccaccggcat atgccggtga ctcccgcttc cgcatatccg gggaggcctc
     2821 gccatggcga aggaagaaca tctcgaaatg aacggtgtcg tcaacgaagt gctgcccgat
     2881 tgccgcttcc gcgtgacttt ggacaacggt catcaactgg ttgcgtattc cgccggcaag
     2941 atgcgcaagc accacatccg tattcttgcg ggtgacaagg tctcgctgga gctctcgccc
     3001 tatgacctga gcaaggggcg gatcaccttc cgccacatcg agacccggcg gccggcctcc
     3061 ccgcagcggc gcagatagtt tcggggcgct gttcgagccg ccgcgcgtgc tcgaacagtc
     3121 attgcgcatc cgcgcggctt tcgcgtccgg acttcattgc ggccccggtc acgacatatt
     3181 gagaacatac tgtccaagtg cagcagattc attcgagcat cgacatcgac gcgcccgcat
     3241 cactggtatg ggccatactg gccgacttcg acgcgtacag gcgctggaac ccatacatcc
     3301 gcgccgtcct tggcaagcca tgcactggaa atgcactgag aatcgccctg caaggtcagg
     3361 ggcgcgacgc tgcgtccatg agttcgatgc tgactcaggt gcgcgagccg cgcgaactgc
     3421 gctggcgcca acgatacgta gcacccggca tttacaccac
//
LOCUS       DBNHBY010000547         5423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1661218, whole genome shotgun sequence.
ACCESSION   DBNHBY010000547 DBNHBY010000000
VERSION     DBNHBY010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1661218"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16HB_13425"
     CDS             complement(<1..442)
                     /locus_tag="AC16HB_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR5607834.1"
                     /translation="MTGTISTATLVVWGGFLLAFVLGFVSNRANFCTMGAISDIMHMG
                     HWGRMRMWLLAIAVAIIGASVLNGLGLVDLSKSIYPRPRLAWLSAIVGGFIFGVGMTL
                     AGGCANKNLLRIGSGSLRSLVVLVFIGISAYMTIKGLFGQWRVDL"
     gene            complement(467..784)
                     /locus_tag="AC16HB_13430"
     CDS             complement(467..784)
                     /locus_tag="AC16HB_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013671.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR5607835.1"
                     /translation="MIEITPAAAERIRAALSGQKDETLALRVAARTGDDGDTEFGMGL
                     DARREQDEEIVTAAGITLLVSPPSLEAVADMVIDFVEVAPGDHRFIFYRAGSLPGDAA
                     PAG"
     gene            complement(798..2186)
                     /locus_tag="AC16HB_13435"
     CDS             complement(798..2186)
                     /locus_tag="AC16HB_13435"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR5607836.1"
                     /translation="MHRLLVSAAHKSSGKTTVAIGLVAALRTRGLAVQPFKKGPDYID
                     PLWLSAAAGRLCRNLDLYLSPPDEVQRAFEASCAGAEVALVEGNKGLYDGLSLDGSNS
                     NAAVAKLLSLPVVLVLDARGMTRGIAPLILGYQAFDRDIRLAGVILNRLGGSRHEAKL
                     RAVIEHYTDVPVLGALHEDPALSIQERHLGLMPATEVAQAQSHIARLANAVRESVDLD
                     RLLASCGTPGAAPAPPGPTTATPDLRVGVARDRAFGFYYAEDLDAFERAGAELVFFDA
                     LADAHLPEVDALFIGGGFPEVLAPELAANRSLLAELRAAIDNGLPTYAECGGLMVLAR
                     SITWRGVTYPMVGAIPADVLMQERPVGRGYVHLQETDSHPWPRGTTATIRAHECHHSK
                     LVNAEPALRFAYKVLRGHGVDGAHDGIVVRNVLASYTHLRSVAGDDWPARFVAFARAV
                     RSKRAQRRASAA"
     gene            complement(2191..3363)
                     /gene="nrfD"
                     /locus_tag="AC16HB_13440"
     CDS             complement(2191..3363)
                     /gene="nrfD"
                     /locus_tag="AC16HB_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5607837.1"
                     /translation="MAGVLISDRSIERRFWLLLLAGAIVLVAGLGAALVMEHEGHVVT
                     GMTNEIVWGTPHVFAIFLIVAASGALNVASVGSVFGQGAYKSRARLSGLLCVALLAGG
                     LMVLMLDLGRPERIVVAATHYNLTSVFAWNVFLYSGMFALVLLYLWTLMERRMNRYSK
                     PAGVAAFIWRFVLTTGTGTIFAFLVARQAYGSAVLPPLFIAMSLSWGMAAFIIAEALL
                     NAWRGGQIPAELLRRMKNLLGVLVVLVLYFTVVYHMTNLYFAKQNAFERFILLDGGVY
                     PLLFWVGYVLFGTIAPLLLIWGPGSGPRGTVLFAAALVIAGAFAQLYVFIIGGQAFPL
                     EIFPGYEVSSSYRDGQVASYAPSLPEVLLGLGGVAAAYLITLVGVQVLDVMPGDGA"
     gene            complement(3363..4091)
                     /gene="dsrO"
                     /locus_tag="AC16HB_13445"
     CDS             complement(3363..4091)
                     /gene="dsrO"
                     /locus_tag="AC16HB_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR5607838.1"
                     /translation="MSERSDTPVSRRALLAGAAAALAGVALAPGVHLVEIARAAGPPA
                     SSKVRWGLLIDANRCSEGCQECVLACNRENGLNGGTRPTDSQWIRKVELKDRKSGKKA
                     SLPVMCQHCAEPPCVDVCPTGASFKRADGIVLVDRHTCIGCRYCMMACPYKARSFVHE
                     PLTTQKPDVPRGKGCVESCTLCVHRIDRGQTTACAEACPTQAIVFGDLNDPNSEISRR
                     IAQVRTTRLRADLNLDTGVQYAGL"
     gene            complement(4088..4456)
                     /locus_tag="AC16HB_13450"
     CDS             complement(4088..4456)
                     /locus_tag="AC16HB_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607839.1"
                     /translation="MRRALLAIALGVAALAAGAEVPRPAISVDKSTKCVAPPEVMRRT
                     HMQMLMHRRDRTVHQGIRGGDEALNRCLECHASKTTGAAIGAPDAFCQSCHDYVGVKL
                     DCFECHQGRPGAAKVTAKQP"
     gene            complement(4459..>5423)
                     /locus_tag="AC16HB_13455"
     CDS             complement(4459..>5423)
                     /locus_tag="AC16HB_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5607840.1"
                     /translation="EVTLTTVFAVDKMQANRHEVEQALAEGIDIQGSLAPIGLVRGPD
                     GRATALRVAECEAKMVGPRLEIKVKEGTERDIPCDLIVSAIGQAVDFTGLEQFDNGKG
                     GVGADRNYQVAGKPGVFAGGDVLRPHLLTTAIGHGAIAADGIDRFLAGEEQEKRPKID
                     VHQFDLLRKYVEKGVTLPPVKEPLHGTDKAPGAIHNFDNRSDRYVIPHGQLFLGHFGF
                     VSRNQRRFITLTRETALGNFEERLDALGEQQAVAEAKRCMSCGLCFECDNCVVYCPQL
                     AVKKVPKKEATTGRYVYTDYDRCVGCHICADVCPTGYIQMGLGE"
BASE COUNT          891 a         1925 c         1777 g          830 t
ORIGIN      
        1 gcaggtcgac gcgccattgt ccgaacaggc ccttgatcgt catgtacgcg gagatgccga
       61 tgaacaccag cacgaccagc gagcgcaggc tgccgctgcc gatgcgcagc agattcttgt
      121 tggcgcagcc gcccgccagc gtcatgccga ccccgaagat gaacccgccg acgatcgccg
      181 acagccaggc gagtcgcggg cgcggataga tcgacttcga caggtcgacg agtccgaggc
      241 cgttgagcac gctcgcgccg atgatcgcca ccgcgatcgc cagcagccac atccgcatcc
      301 ggccccaatg gcccatgtgc atgatgtcgg agatcgcgcc catggtgcag aagttcgcgc
      361 ggttcgacac aaagccgagc acgaatgcaa gcaggaaccc gccccagacg acgagggtgg
      421 cggtgctgat ggttccggtc atggcctgtg cgctctcccc gttctcttat ccggcgggcg
      481 ccgcatcgcc gggcaacgag ccggcgcgat agaagatgaa ccgatgatcg cccggcgcga
      541 cctcgacgaa gtcgatcacc atgtcggcga ccgcctccag cgacggcggc gacaccagca
      601 gcgtgatgcc tgccgcggtg acgatctcct cgtcctgttc gcgccgcgcg tcgagcccca
      661 tgccgaactc ggtgtcgccg tcgtcgcctg tgcgcgcggc cacgcgcagc gcgagcgttt
      721 cgtccttctg cccgctcagc gcagccctga ttcgctcggc cgcggccggc gtgatctcga
      781 tcatcgccgg gctccggtca cgccgccgag gcgcggcgct gggcgcgctt cgacctgacc
      841 gctcgggcga aggcgacgaa ccgcgccggc cagtcatcgc cggcgacgct gcgtagatgc
      901 gtgtacgacg ccagcacgtt gcgtacgacg atgccgtcgt gcgcgccgtc gacgccgtgg
      961 ccgcgcagca ccttataggc gaaacgcagc gccggctcgg cgttgacgag cttcgagtgg
     1021 tggcactcat gggcgcggat cgttgcggtc gtaccgcgcg gccacggatg ggaatcggtc
     1081 tcctgcaggt gcacgtagcc gcgtcccacg gggcgctcct gcatcagcac gtcggccggt
     1141 atcgcgccga ccatcgggta cgtgaccccg cgccacgtga tcgagcgcgc cagcaccatc
     1201 agtccgccgc actccgcgta cgtcggcaag ccgttgtcga tcgcggcgcg caactcggcc
     1261 agcaaactgc ggttggcagc gagttcgggc gcgagcactt cggggaatcc gccgccgatg
     1321 aacagcgcgt cgacttcggg caggtgcgcg tccgccagtg catcgaagaa caccagttcg
     1381 gcgccggcgc gttcgaatgc gtcgagatcc tccgcgtaat agaagccgaa ggcgcggtcg
     1441 cgcgcgacgc cgacgcggag gtccggcgtg gccgtcgtcg ggcctggtgg cgcgggcgcg
     1501 gcgcctggcg tgccgcagct cgcgagcagc cggtcgagat cgaccgactc cctgaccgcg
     1561 ttcgcgagcc gcgcgatgtg cgattgcgcc tgcgcgactt cggtcgccgg catcaggccg
     1621 aggtggcgtt cctggatcga gagcgccggg tcctcgtgca gcgcaccgag caccggcacg
     1681 tcggtgtagt gctcgatcac cgcgcgcagc ttggcttcgt gacgcgaccc gccgaggcga
     1741 ttcaggatca cgccggcgag ccggatgtcg cggtcgaagg cctggtagcc caggatcagg
     1801 ggcgcgatgc cgcgcgtcat gccgcgcgcg tcgagcacca gcaccaccgg aaggctcaag
     1861 agcttcgcca ccgccgcgtt cgaattgctg ccgtcgagcg acagtccgtc gtacagcccc
     1921 ttgtttccct cgaccagcgc gacctccgcg cccgcacagc tggcctcgaa cgcgcgctga
     1981 acctcgtccg gcggcgaaag gtacaggtcg aggttgcggc ataagcgccc cgccgcggcg
     2041 gacaaccaca gcggatcgat gtagtcgggt cccttcttga acggctgcac ggcgaggccg
     2101 cgcgtgcgaa gcgccgcgac caggccgatc gcgaccgtcg tcttccccga cgacttgtgc
     2161 gcggccgaga cgagcaaccg gtgcatggtt ctatgcgccg tcgcccggca tcacgtcgag
     2221 cacctgcacg ccgaccagcg tgatcaggta cgcggcagcc acgccgccca ggccgagcag
     2281 cacctccggc aggctgggcg catagctggc gacctgtccg tcgcggtagc tgctcgaaac
     2341 ctcgtagccc gggaagatct cgagcggaaa cgcctgcccg ccgatgatga acacatacag
     2401 ttgcgcaaac gcgccggcga tcaccagcgc ggcagcgaac agcaccgtgc cgcgcgggcc
     2461 gctacccggc ccccagatca gcaacagcgg cgcgatggtt ccgaacagca cgtagccgac
     2521 ccagaataga agcggataca cgccaccgtc gagcaggatg aagcgctcga acgcgttctg
     2581 tttcgcgaag tacaggttcg tcatgtgata gacgaccgtg aagtacagca ccagcaccac
     2641 cagtacgccg agcaggttct tcatccgccg cagcagttcg gccggtatct gaccgccgcg
     2701 ccatgcgttg agcagcgcct cggcgatgat gaacgctgcc atgccccacg acagcgacat
     2761 cgcgatgaac agcggcggca gcaccgccga gccgtatgcc tgccgcgcga ccaggaacgc
     2821 gaagatcgtg ccggtaccgg tggtcagcac gaagcgccag atgaatgccg ccacgcccgc
     2881 cggcttgctg tagcggttca ttcgcctttc catcagcgtc cagaggtaga gcagcacgag
     2941 cgcgaacatg cccgagtaca ggaacacgtt ccacgcgaac accgacgtga ggttgtaatg
     3001 ggtcgccgcc acgacgatgc gctccggccg ccccaggtcg agcatcagca ccatcagccc
     3061 gccggccaac agcgcgacac acagcagtcc cgacagccgc gcgcgcgact tgtacgcacc
     3121 ctgcccgaac acgctgccga ccgatgccac gttcagcgcc ccggaggcgg cgacgatcag
     3181 gaagatcgcg aacacgtgcg gcgtgcccca cacgatctcg ttggtcatgc cggtgacgac
     3241 gtgcccttcg tgctccatta ccaacgccgc gccgagcccg gccacgagca cgatcgcgcc
     3301 cgcgagcagc agcagccaga aacggcgctc gatcgaacga tccgaaatca ggacgcctgc
     3361 catcacagtc ccgcgtactg cacaccggtg tcgaggttca ggtccgcgcg caaccgcgtg
     3421 gtgcggacct gcgcgatgcg gcgcgagatc tccgagttcg gatcattcag atcgccgaac
     3481 acgatcgcct gggtcgggca cgcctctgcg cacgcagtcg tctggccacg gtcgatccgg
     3541 tgcacgcaca gcgtgcacga ctccacacaa cccttgccgc gcggcacgtc gggcttctgc
     3601 gtggtcagcg gctcgtgcac gaagctgcgt gccttatatg ggcacgccat catgcagtag
     3661 cggcagccaa tgcaggtgtg gcgatcgacc agcacgatgc cgtcggcgcg cttgaacgac
     3721 gcaccggtcg ggcacacgtc gacgcacggc ggctcggcac agtgctggca catcaccggc
     3781 aggctcgctt tcttcccgct cttgcggtcc ttcaactcga ccttgcggat ccactgcgaa
     3841 tcggtcggcc gcgtcccgcc gttcaggccg ttctcgcgat tgcatgcgag cacgcattcc
     3901 tggcagccct cgctgcaccg gttggcgtcg atcagcaggc cccagcggac cttcgacgac
     3961 gccggcggcc cggcagccct cgcgatctcg accagatgca cgccgggcgc gagcgcgact
     4021 cccgccagcg cggcggcggc acccgcgagc agcgcgcggc gcgagaccgg ggtatcggag
     4081 cgctcgctca tggctgtttc gccgtcacct tcgccgcccc cggccgtccc tgatggcatt
     4141 cgaagcagtc gagcttcacg ccgacgtagt cgtggcatga ctggcagaac gcgtcgggcg
     4201 cgccgatcgc cgcgccagtg gtcttgctgg cgtggcactc gaggcagcgg ttcaacgctt
     4261 cgtcaccgcc gcggatgccc tggtggacgg tgcggtcgcg ccgatgcatc agcatctgca
     4321 tgtgcgtgcg gcgcatgacc tcgggcggcg cgacgcattt cgtgctcttg tcgaccgaaa
     4381 ttgcagggcg cggcacctcg gcgccggcgg ccagcgccgc gacgcccaga gcgatcgcga
     4441 gcagcgcccg gcgcatcgct actctccgag ccccatctgg atgtagccgg tcgggcagac
     4501 atcggcgcag atgtggcagc ccacgcagcg gtcgtagtcg gtgtacacgt agcggccggt
     4561 ggtcgcttcc ttcttcggca ctttcttcac ggcaagctgc gggcagtaca cgacacagtt
     4621 gtcgcactcg aagcacaggc cgcaactcat gcagcgcttg gcttcggcga ccgcctgctg
     4681 ctcgccgagc gcgtcgaggc gctcctcgaa gttgccgagc gcggtttcgc gcgtcagcgt
     4741 gatgaacctg cgctgattgc gcgaaacgaa gccgaagtgc ccgaggaaaa gctgtccgtg
     4801 cgggatcacg tagcgatccg accggttgtc gaagttgtgg atcgcgccgg gcgccttgtc
     4861 ggtgccatgc agcggttcct tcacgggcgg cagcgtcacg cccttctcga cgtacttgcg
     4921 cagcaggtcg aactgatgca cgtcgatctt cggccgcttc tcctgctcct cgcccgcgag
     4981 gaagcggtcg atgccatccg cggcgatggc gccgtggccg atcgcggtcg tcagcagatg
     5041 cggccgcaac acgtcgccgc cggcgaacac ccccggcttg cccgcgacct ggtagttgcg
     5101 gtcggcgccg accccaccct tgccgttgtc gaactgctcc agccccgtga aatcgacggc
     5161 ctgcccgatt gccgacacga tgaggtcgca cgggatgtcg cgctcggtgc cttccttgac
     5221 cttgatttcc agccgcggcc cgaccatctt cgcctcgcac tcggccacgc gcagcgcggt
     5281 ggcgcgcccg tcggggccgc gcacgaggcc gatcggtgcc aagctgccct ggatgtcgat
     5341 gccttcggcc agcgcctgct cgacctcgtg cctgttggcc tgcatcttgt cgaccgcgaa
     5401 cacggtcgtc agcgtcactt ccg
//
LOCUS       DBNHBY010000548         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1592729, whole genome shotgun sequence.
ACCESSION   DBNHBY010000548 DBNHBY010000000
VERSION     DBNHBY010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1592729"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..463
                     /locus_tag="AC16HB_13460"
     CDS             59..463
                     /locus_tag="AC16HB_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5607841.1"
                     /translation="MKILGIDHIVLRCRDAARMERFYVDVLGCTVDKRRDDLGLVHLR
                     AGSGLIDLVDIGGKLGRGGAAPVAGAHNVDHVCLRIEPFDAAALERHLAAFGIARGEV
                     HTNYGAEGNGPSIYLADPEGNLVELKGPATSA"
     gene            complement(491..>1667)
                     /locus_tag="AC16HB_13465"
     CDS             complement(491..>1667)
                     /locus_tag="AC16HB_13465"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5607842.1"
                     /translation="FDAFTCTECGRCKTSCPTFLTGKPLSLKWVHDSVKHHLIAERER
                     IVHAHEEALPPLIGKVIGDDTLWACTTCGYCEVACPIELEHLGKFYRLRQHRVMMDGE
                     FPHELKSVFEAYEATSNPWGLPQQARADWAKGLEVPIVTTREQVQALDYLFYVGSAQS
                     YDPRGQKIARAFVQILRAANVKFGILGAAETSTGECVRRAGNEMLFQVLAPQLIETLN
                     GLGVTRIVTCDPHVFNTLKNEYPAFGGKYEVIHHTQLIARLLAEGRLKVAPQFERVML
                     HDPCYLGRHNGEFDAPRAVLARLSRDAPIEFELSRERAMCCGAGGGRMWMEESIGTRI
                     NVARVEQALPLAPATIATACPYCAVMMADGLAAVGQAEKVDTRDIAELVAAALATAA"
BASE COUNT          256 a          570 c          565 g          276 t
ORIGIN      
        1 cagacaagca gcgctgggct gcgcaactcg acgcctacat ggcggcacag ggagtggcat
       61 gaagatcctc ggcatcgatc acatcgtcct gcgctgccgc gacgccgcgc ggatggagcg
      121 tttctacgtc gacgtgctcg gctgcacggt cgacaagcgg cgcgacgatc tcggcctggt
      181 gcacctgcgc gcgggcagcg ggctgatcga tctcgtcgac atcggcggca agctcggccg
      241 gggcggcgcg gcgccggtcg ccggcgcgca caacgtcgat catgtgtgcc tgcggatcga
      301 accgttcgac gccgcagcgc tggagcgcca tctcgctgcg ttcggcatcg cgcgcggcga
      361 ggtgcacacc aactacggcg ccgaaggcaa cggcccgtcg atctaccttg ccgacccgga
      421 gggcaatctg gtcgagctaa agggaccggc cacgtcggcg taactggcgg cgctcgacgg
      481 caaatggcgg tcaggccgcg gtcgccagcg cagccgcgac cagttcggcg atgtcgcgcg
      541 tgtcgacctt ctccgcctgg ccgacagccg cgaggccatc ggccatcatc accgcgcagt
      601 aggggcacgc ggtcgcgatc gtcgcaggcg cgagcggcaa cgcctgctcg acgcgcgcga
      661 cgttgatgcg cgtgccgatg ctctcttcca tccacatgcg gccgccgccg gcgccgcagc
      721 acatcgcgcg ctctcggctg agctcgaatt cgatcggcgc atcgcgcgac agccgcgcca
      781 gcactgcgcg cggtgcgtcg aactcgccgt tgtggcggcc gaggtagcac gggtcgtgca
      841 gcatcacgcg ctcgaactgc ggcgcgacct tcagccgccc ttcggcgagc agccgcgcga
      901 tcagctgcgt atggtggatg acttcgtact tgccgccgaa cgccggatac tcgttcttca
      961 gcgtgttgaa cacatgcggg tcgcaggtga cgatacgcgt gacgccgagg ccgttcagcg
     1021 tttcgatcag ctgcggcgcc agcacctgga acagcatctc gttgccggcg cggcgcacgc
     1081 actcgccggt cgacgtttcg gccgcgccga ggatgccgaa tttcacgttt gccgcgcgca
     1141 ggatctgcac gaacgcgcgc gcgatctttt gcccgcgcgg gtcgtacgac tgcgccgagc
     1201 cgacgtagaa caggtagtcg agcgcctgca cctgctcgcg cgtcgtgacg atgggcacct
     1261 cgagcccctt ggcccagtcg gcgcgcgcct gctgcggcag tccccacgga ttgctggtgg
     1321 cctcgtacgc ctcgaagacg cttttcagct cgtgcgggaa ctcgccgtcc atcatcacgc
     1381 ggtgctgccg cagccgatag aacttgccga ggtgttccag ttcgatcggg cacgcgactt
     1441 cgcagtagcc gcaggtcgtg catgcccaca gcgtgtcgtc gccgatcacc ttgccgatca
     1501 gcggcggcaa tgcttcctcg tgcgcgtgca cgatgcgttc gcgctcggcg atcagatggt
     1561 gcttgacgct gtcgtggacc catttgagcg acagcggctt gccggtcagg aacgtcggac
     1621 acgaagtctt gcagcggccg cactcggtgc acgtgaaggc gtcgaac
//
LOCUS       DBNHBY010000549         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_984251, whole genome shotgun sequence.
ACCESSION   DBNHBY010000549 DBNHBY010000000
VERSION     DBNHBY010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_984251"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..490
                     /locus_tag="AC16HB_13470"
     CDS             209..490
                     /locus_tag="AC16HB_13470"
                     /inference="COORDINATES: protein motif:HMM:NF042524.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HIR5607843.1"
                     /translation="MWRRIRGLGAVYLQNGIRVLPTTTDHERQFRIVQSEIVRAGGEA
                     VLEAYASEVFEREHSAKGALQKGPAAAKGRITRPPAATKARARRSNQRG"
     gene            complement(525..944)
                     /locus_tag="AC16HB_13475"
     CDS             complement(525..944)
                     /locus_tag="AC16HB_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5607844.1"
                     /translation="MIRRILLGYDGSDISAEAFAFALDLARKYGAELHVIAVARPPEY
                     AEDVEAEAVIENSRRHYDKVLGVLRPKLAGEPIKSELHVVVGHPAEQIVRYAEGHGID
                     HIVVGHRGHTRFDRWLIGSVARQVIAYAHCAVTVLRK"
     gene            complement(941..>1779)
                     /locus_tag="AC16HB_13480"
     CDS             complement(941..>1779)
                     /locus_tag="AC16HB_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR5607845.1"
                     /translation="MSTTSVAVVYAVMLEFGLNATHYGKVVLAACFITDLATVVALGL
                     IFAPFTWKTLVFLGAGATVFLVLPWLTPRFFRRYGGRPSELEAKFLMFALFGLGALAT
                     WADSEAVLPAYLLGMVLAGTVGKDHALIRRLRTLTFGLLTPFYFIRAGSLVSLPALIA
                     APAAFLVLLAAKMVTKVIGVYPVTRLYRSPRQEGMYTTLLMSTGLTFGTISALFGLSH
                     QLIDQAQYSALVAAVIGSAVIPTLIANAFFLPHHLLPRNEPDAPAVAPLPEPQTSLRR
                     TP"
BASE COUNT          325 a          606 c          610 g          238 t
ORIGIN      
        1 gcgtggcaag ccccgccgcc gcgctacacg cgcggcgtga tggcgaaatt cgcgatgaat
       61 gcgtcgagcg ccagcacggg ggcggtgctc gaccggttcg ggccgccggc caggtgagtc
      121 tgacccgagc ggtgcagcgg caggcgtcct cctggctgct gctgacctac aaggtcccgc
      181 gcccgaacct tccaagctgc gcgtcgcgat ctggcggcgt atccgcggtc tgggcgcggt
      241 gtacctgcag aacggcatcc gcgtgctgcc gacgacgacg gatcacgagc gccagttccg
      301 catcgttcag tcggagatcg tgcgcgctgg cggcgaggcg gtgctcgagg catatgcgag
      361 cgaagtcttc gagcgcgagc acagtgcgaa gggcgcactg cagaaaggcc ccgccgcggc
      421 gaaggggcga atcacccggc cgccggccgc aacgaaggcg cgggcacgcc gaagcaacca
      481 gcggggctga ccgatcgcac cggggcgcgg cccggcgagg accactactt acgcagcacc
      541 gtcaccgcgc agtgcgcgta cgcgatcacc tggcgcgcca ccgaaccgat cagccagcgg
      601 tcgaaccgcg tgtggccgcg gtggccgacg acgatgtggt cgatgccatg cccctcggcg
      661 tagcgcacga tctgctccgc gggatggccg acgacgacat gcagttcgct cttgatcggc
      721 tcgccggcga gcttcggccg cagcacaccg aggaccttgt cgtagtgccg gcgcgagttc
      781 tcgatcaccg cctcggcctc gacatcctcg gcgtactccg gtggccgcgc gaccgcgatc
      841 acgtgcagtt cggcgccgta cttgcgcgcg aggtccagcg cgaatgcgaa cgcctcggcg
      901 gaaatatcgg agccgtcgta gcccagcaga atgcgtcgga tcatggcgtc ctccggagcg
      961 aagtttgcgg ttcgggcagc ggcgccaccg ccggcgcgtc gggctcgttc cgcggcagca
     1021 ggtggtgcgg caggaagaac gcattggcga tcagcgtcgg tatcaccgcg ctgccgatga
     1081 ccgcggccac cagcgcggag tactgtgcct ggtcgatcag ttgatgcgac aagccgaaga
     1141 gggccgaaat cgttccgaaa gtcagccccg tcgacatcag cagcgtcgtg tacatgccct
     1201 cctgacgcgg cgaccgatag agccgagtga ccgggtagac gccgattacc ttggtcacca
     1261 tcttcgccgc cagcaacaca agaaaggcgg ccggcgccgc gatcagcgcc ggcaacgaca
     1321 ccagcgatcc ggcgcggatg aagtagaacg gcgtcagcag cccgaaggtg agcgtgcgca
     1381 accggcggat cagcgcgtgg tccttgccca cggtgccggc gagcaccatg ccgagcaggt
     1441 aggcaggcag caccgcttcg ctgtcggccc atgtcgccag cgcgccgagg ccgaacagcg
     1501 cgaacatcag gaacttcgct tccagctccg acggccgccc gccgtaacgc ctgaagaagc
     1561 gcggcgtcag ccacggcagc accaagaaca cggtcgcgcc cgcaccgagg aacaccagcg
     1621 tcttccaggt gaacggcgcg aagatcaggc cgagcgccac cacggtcgcg agatccgtga
     1681 tgaagcaggc ggcaagcacc accttgccgt agtgcgttgc gttcaaaccg aactcgagca
     1741 tcaccgcgta gacgaccgcg accgacgtgg tcgacatcg
//
LOCUS       DBNHBY010000550         7407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_375401, whole genome shotgun sequence.
ACCESSION   DBNHBY010000550 DBNHBY010000000
VERSION     DBNHBY010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_375401"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="AC16HB_13485"
     CDS             complement(<1..95)
                     /locus_tag="AC16HB_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607846.1"
                     /translation="MAERNIGEVILRVENIALAFGGVKALTDVSF"
     gene            complement(100..357)
                     /locus_tag="AC16HB_13490"
     CDS             complement(100..357)
                     /locus_tag="AC16HB_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607847.1"
                     /translation="MTTKNWLAVLCLIAGIPLAAYGLANGSWLLAIPGLALVLVFWYL
                     IWQWFAGAGRPMGAEGSIKPAANAAWKMKDEPAPRGQDRKE"
     gene            complement(378..2333)
                     /locus_tag="AC16HB_13495"
     CDS             complement(378..2333)
                     /locus_tag="AC16HB_13495"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR5607848.1"
                     /translation="MAAPSSATPDTFPRLLLQHAYLRAGHPALREKDLGIWQTWTWAR
                     VADEVKKLAAGLHVQGFRRGDHLAIIGENRPRLYFAMMATQCLGGVPVPMYQDAVASE
                     MVYVFENAKIAYAIVEDQEQVDKMLEIREQHSRLEHIYYDDPRGLRHYTNPGLLPYEE
                     LIRLVEGLLSVQPKFIDEEIDKGSGDDTAALFYTSGTTGKPKGVVQTHRALIDRARAC
                     TDMEGLTVYEDVLAYLPMAWIGQNIFSYAQALVTGYTVNCPESPATVTTDMREIGPTY
                     YFAPPRVFEGLLTQVMIRMEDAGRLKRSMFHYFMGVAKRVGGKILDKQPVGFVERTLY
                     SLGDVLVFGPLRNVLGMSRIKVAYTAGEAIGPDLFTFYRSIGINLKQLYGSTETAVFV
                     CVQPNGQVKPDTVGPPIAGVELRIEDTGEILLKSPGLLKEYYKNPDATREVLDTDGWY
                     HTGDAGYLDQDGHLKIIDRASDVGRLGDGTLFAPKYIENKLKFFPHIKEAVAFGNGRD
                     MACAFINIDFEAVGNWAERRGLPYAGYIDLAGKREVYDLIADCVEKVNADLAADPKLA
                     NSQIHRFLLLHKELDPDDDELTRTRKVRRRFIQDKYGVLVDAMYAGSKEQFVETQVKF
                     EDGRIGKLSATVAIRDARVFQPLAKAA"
     gene            2489..3169
                     /locus_tag="AC16HB_13500"
     CDS             2489..3169
                     /locus_tag="AC16HB_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5607849.1"
                     /translation="MLRETLDEYLSATHWGTLLTPEQRVRVSAESSETVVPSEGFVCR
                     RGEPVDAWLGVLDGLVKMMITAPDGKTSTFTGIPAGGWFGEGSLLKTEPRRYDVVALR
                     ESRIVRVPRRTFEWLLNTSIPFNRFLLVQLNERLGQFIGMLEAQRLHGPDARVALCVA
                     ALFNPILYPGIEKKLEVSQEEIGYLAGISRQRVNQALHTLEQARLIRVEYGAILIEDL
                     EGLRHYKG"
     gene            complement(3175..3621)
                     /locus_tag="AC16HB_13505"
     CDS             complement(3175..3621)
                     /locus_tag="AC16HB_13505"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5607850.1"
                     /translation="MESIVTIIDIAQPPTKVFEFVTNAAQWKRWHPATMAVSGVPNRP
                     LEVGETIDEQIHAGPRRFSARWTVIECKPPRRWVIVTDSNEGEARITYKLSEQDGVCY
                     FERTLEYRSKRLPWKWFDGSLTRWALTRQSDQALRNLKRVIESRST"
     gene            complement(3633..3938)
                     /locus_tag="AC16HB_13510"
     CDS             complement(3633..3938)
                     /locus_tag="AC16HB_13510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5607851.1"
                     /translation="MNGEERVVHVMARIVARADATEALKRVLSDLVEPSRREPGCVAY
                     ALYQRAEAPHEFQTIEQWRSQADADAHMATPHVQAAVAAALPLLAQPPAIHAYALIR"
     gene            4117..4950
                     /locus_tag="AC16HB_13515"
     CDS             4117..4950
                     /locus_tag="AC16HB_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5607852.1"
                     /translation="MKTVSLPSGELVPALGLGTWRMGERARSRAQEIAAVRAAVDLGY
                     RLIDTAEMYGEGGAEHVIGEAIADRRDGIFLVSKFYPHNASRTAAVAACERSLKRLRT
                     DRIDLYLLHWRGAVPLAETVEAFEHLKRDGKIRHWGVSNFDTADMDELWRLAPGPRCA
                     ANQIYYSVSRRGIEFDLLPWQRSRGVPAMAYCPIDEGALAQNVALAAIGKPHHATAAQ
                     VALAWAISRPGVIAIPKAVDPKHLRENLAAAELRLSADDLAQIDRDFPPPRKRTRLAI
                     V"
     gene            complement(4957..6168)
                     /locus_tag="AC16HB_13520"
     CDS             complement(4957..6168)
                     /locus_tag="AC16HB_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607853.1"
                     /translation="MESSSKQNWWLVTLLAAALLMVTMGARQSLGLIVSPLNTSTGLG
                     IVTISFAMAVGQFVWGAVQPVAGAIADRYGPGRVLAAGLVILALGSALTPLMTTGAGL
                     VFSLGVLSAIGSGAGSFSVLIGAAAQRVSVEHRGTASGVINAGGSFGQFVFAPVLQKL
                     INVFGWMGAMWSLAAITLAALPVARALRRAPSAHVEARHADGGLRAQLRTAFGDRSYL
                     LLHAGFFTCGFHIAFLVTHLPGEVSLCGLPASVASWSLALIGLANIAGSLFAGWCVNR
                     YRSKYILAWMYGSRTVLVLLYLLAPKTEWTFYLFAIGLGFTWLATVPPTAGVVAKLFG
                     VRYLATLFGLTLLSHQIGGFFGAWLGGIAIAQQGSYLWMWYADAVLAAAAAVCNLPIR
                     EARVQRVVQPV"
     gene            6248..6638
                     /locus_tag="AC16HB_13525"
                     /pseudo
     CDS             6248..6638
                     /locus_tag="AC16HB_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(6645..7160)
                     /locus_tag="AC16HB_13530"
     CDS             complement(6645..7160)
                     /locus_tag="AC16HB_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HIR5607854.1"
                     /translation="MQSLIRSTAVALGAVLLALAPPVPARQSDPIPTVTRLVKLYLER
                     EDQMLAAMRARDAAALERMLADDFELRVAARPGVPVPRADFLQRVGTEATGAYTIEQM
                     AVHEIGTTSIFSFMLRPAARDRSTPPLFVVDTWVGGADDARLRVRYVAPAAAARIPDA
                     AADTPQIPKKY"
     gene            complement(7219..>7407)
                     /locus_tag="AC16HB_13535"
     CDS             complement(7219..>7407)
                     /locus_tag="AC16HB_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607855.1"
                     /translation="PNLNAWYDRRGYENADKCAWTFGTTSTASNGSKYNMTLGSRQYL
                     IQRNWVNAGGGYCATAYP"
BASE COUNT         1228 a         2560 c         2394 g         1225 t
ORIGIN      
        1 tcgaaggaga cgtcggtcaa ggcctttacg ccgccgaagg cgagcgcgat gttctcgacc
       61 cgcaggatca cttcgccgat gtttcgctct gccatgcggc tactctttcc tgtcctgccc
      121 gcgcggcgcg ggttcgtcct tcatcttcca tgctgcgttc gccgccggct tgatcgagcc
      181 ttccgcgccc atcggcctgc cggcgccggc gaaccattgc cagatcaggt accagaacac
      241 cagcaccagc gcgaggccag gtatcgccag cagccaggag ccgttcgcca gcccgtaggc
      301 ggccagcgga atccctgcga tcaggcacag gacggcgagc cagttcttcg tcgtcacgtc
      361 cgctccgctg cgtcccgtca ggccgccttc gccagcggct gaaacaccct ggcatcgcgg
      421 atcgccaccg tggcgctgag ctttccgatg cggccgtcct cgaacttgac ctgcgtctcg
      481 acgaactgct ccttgctccc cgcatacatc gcgtcgacca gcaccccata cttgtcctgg
      541 atgaaccggc gccgcacctt gcgcgtgcgc gtcagttcgt cgtcgtccgg gtcgagttcc
      601 ttgtgcagga gcagaaagcg gtggatctgc gagttggcga gcttcggatc ggccgccaga
      661 tccgcattga ctttctcgac gcagtcggcg atcaggtcgt agacctcgcg cttgccggcc
      721 aggtcgatgt aacctgcgta cggcagcccg cgccgctcgg cccagttgcc aaccgcctcg
      781 aagtcaatgt tgatgaacgc gcacgccatg tcgcgcccgt tgccgaaggc gaccgcttcc
      841 ttgatgtgcg ggaagaactt cagcttgttc tcgatgtatt tcggtgcaaa cagcgtgccg
      901 tcgccgagtc gcccgacgtc gctcgcgcgg tcgatgatct tcaggtgccc gtcctggtcc
      961 aggtagccgg catcgcccgt gtgataccag ccatcagtgt ccagcacttc gcgcgtcgca
     1021 tcgggattct tgtagtactc cttgagcagc ccgggactct tcagcaggat ctcaccggtg
     1081 tcctcgatcc tcagttcgac gccggcgatc ggcggaccga ccgtatcggg cttgacctgg
     1141 ccgttgggtt gtacgcacac gaacaccgcg gtctcggtgg agccgtacag ttgcttcagg
     1201 ttgatgccga tcgagcgata gaacgtgaac aggtccgggc cgatcgcctc gccggccgta
     1261 taggcgacct tgatgcggct catcccgagc acattgcgca gcggcccgaa caccagaacg
     1321 tcgccgagcg aatagagcgt ccgctcgacg aaacccactg gctgcttgtc gagaatcttc
     1381 ccgccgacac gcttggccac gcccatgaag tagtgaaaca tgctccgctt cagacggccc
     1441 gcgtcctcca tgcggatcat cacctgcgtc agcagcccct cgaacacgcg cggcggcgcg
     1501 aagtagtagg tcggaccgat ctcgcgcatg tcggtcgtga ccgtggctgg cgactcgggg
     1561 cagttgaccg tgtagccggt gaccagcgcc tgcgcgtacg agaagatgtt ctgtccgatc
     1621 cacgccatgg gcagataggc gagcacatcc tcgtagaccg tcagcccctc catgtccgtg
     1681 cacgcgcgcg cgcggtcgat cagcgcgcga tgcgtctgca ccacgccctt gggcttgccg
     1741 gtcgtccccg acgtgtagaa cagcgccgcc gtgtcgtcac ccgagccttt gtcgatctcc
     1801 tcgtcgatga acttcggctg caccgacagc aggccttcga ccagccgaat cagctcttcg
     1861 tagggcagca ggccggggtt cgtgtagtgg cgcagtccgc gcgggtcgtc gtagtagatg
     1921 tgctcgagcc gggaatgctg ctcgcggatc tcgagcatct tgtcgacctg ctcctggtct
     1981 tcgacgatcg cgtaggcgat cttggcattc tcgaacacgt acaccatttc ggacgcgacc
     2041 gcgtcctggt acatcggcac cggaacgccg cccaggcact gtgtcgccat catcgcgaag
     2101 tacaaccgcg ggcggttctc gccgatgatc gccaggtggt cgccgcgccg gaagccctgc
     2161 acgtgcaggc cggccgcaag cttcttcacc tcgtcggcga cccgcgccca ggtccaggtc
     2221 tgccagatgc ccagatcctt ttcgcgcagc gccggatggc cggcgcgcag gtaggcatgc
     2281 tgcagcagca gtcgcggaaa cgtgtccggt gttgcagagg atggcgccgc catgctccgt
     2341 gtctcctatc tgcccctggt tcgtgccgcg ctcgagcgcg gtcttccgtc tctggcgccg
     2401 gaaaggtagc accgacgccg gcggaaagcg aggcagcacg gtagccagcc cggctgcgcc
     2461 gcgctgtcat ccgggcgaca atcgcccaat gctccgggaa actcttgacg aatatctatc
     2521 cgccacgcat tggggcacgc tgctcacgcc cgagcagcgc gtccgtgtat cggcggagtc
     2581 gagcgagacc gtcgtcccca gcgagggctt tgtctgccgg cgcggcgagc ccgtcgacgc
     2641 ctggctcggt gtgctcgacg gcctggtcaa gatgatgatc accgcgccgg acggcaagac
     2701 gtcgaccttc accggcatcc cggccggcgg ctggttcggg gagggcagcc tgctgaagac
     2761 cgagccgcgc cgctacgacg tggtcgcgct gcgcgagtcg cgcatcgtgc gcgtgccgcg
     2821 ccgcacgttc gaatggctcc tgaacaccag catcccgttc aaccgcttcc tgctggtaca
     2881 gctgaacgaa cggctcggcc agttcattgg catgctggag gcgcagcgtc tgcacggtcc
     2941 ggacgcgcgc gtcgcgctgt gcgtggcggc gctattcaat ccgatcctgt atcccgggat
     3001 cgagaagaaa ctcgaagtct cgcaggaaga gatcggctat ctagccggca tctcgcgcca
     3061 gcgcgtcaac caggcgctgc acacgctgga gcaggcccgg ctgatccgtg tcgaatacgg
     3121 cgcgatcctg atcgaggatc tggagggact gcgccactac aaggggtagc ccggtcacgt
     3181 cgagcgcgac tcgatcacgc gcttcaaatt gcgcagcgcc tgatcggact ggcgcgtcag
     3241 cgcccagcgc gtcagcgagc catcgaacca cttccaaggc aaccgtttgg accggtactc
     3301 cagcgtgcgt tcgaaatagc agacgccgtc ctgctcgctc aacttgtacg tgatcctggc
     3361 ttcgccttcg ttcgaatcgg tcacgatcac ccaacggcgc ggcggtttgc actcgatcac
     3421 cgtccagcgc gccgaaaaac ggcgcgggcc ggcgtggatc tgctcgtcga tcgtttcgcc
     3481 cacctcgagc gggcggttcg gcacgccgct gaccgccatc gttgccggat gccagcgttt
     3541 ccactgcgca gcgttggtga cgaactcgaa cactttcgtc ggcggctgcg cgatatcgat
     3601 gatggtgacg atgctctcca cggttcgccc cgctaccgga tcagcgcata cgcgtggatg
     3661 gccggcggct gcgcgagcag cggcagcgcg gccgcgacgg cggcctgcac gtgcggcgtg
     3721 gccatgtgcg catcggcgtc ggcctggctg cgccattgct cgatcgtctg gaattcgtgc
     3781 ggcgcttcgg cgcgctgata gagcgcatac gcgacacagc ccggctcgcg gcgcgaaggc
     3841 tcgaccagat cggacagcac ccgtttcaag gcttcggtcg catcggcgcg ggcgacgatg
     3901 cgcgccatca cgtgaaccac gcgttcctct ccattcattc tgtcggctcc ctgcgatacg
     3961 gtcgattcgc ggcgccggat tcatgctccc cgggggcggt atatatgcgc cagcttgcgc
     4021 gacagatcaa gccctgcctt gctgtcgccg ctcgcgaata cgtgcggccc cggcgcgggc
     4081 gagtatcttc ggcatggcga cgcgggggga ttcgagatga agacggtcag tctgccctcg
     4141 ggcgagttgg tgccggcgct cgggctgggc acatggcgga tgggcgaacg cgcgcgctca
     4201 cgagcgcagg aaatcgctgc cgtgcgcgcc gccgtcgacc tgggctatcg gttgatcgac
     4261 accgccgaga tgtacggtga gggcggtgcg gaacacgtga tcggcgaggc gattgccgac
     4321 cgccgtgacg gcatattcct cgtcagcaag ttttacccgc acaacgcttc gcgcacggca
     4381 gcggtcgccg cgtgcgaacg cagcctgaag cggttgcgca cggatcggat cgacctgtac
     4441 ctgctgcact ggcgcggggc cgtgccgctg gcagagacgg tcgaggcatt cgagcacctt
     4501 aagcgggacg gcaagatccg gcactggggc gtcagcaatt tcgataccgc cgacatggac
     4561 gaattgtggc ggctggcgcc gggcccgcgg tgcgcagcca accaaatcta ttactccgtg
     4621 tcccggcgcg gcatcgagtt cgacctgctg ccatggcaac ggagccgcgg cgtgccggcg
     4681 atggcctatt gcccgatcga cgaaggcgcg ctggcgcaga acgtcgcact ggccgcgatc
     4741 ggcaagccgc accacgcgac cgcggcgcag gtcgcgctgg cgtgggcgat ctcgaggccg
     4801 ggggtgatcg caattccgaa ggcggtcgac ccgaagcacc tgcgcgagaa cctcgccgcc
     4861 gccgagcttc gcttgagcgc cgacgatctg gcgcagatcg accgtgattt cccgccgcca
     4921 cgcaagcgca cgcggctggc gatcgtttga accggactac accggctgga cgacgcgctg
     4981 cacccttgct tcgcggatcg gcaggttgca gaccgcggcc gcggccgcaa gcaccgcgtc
     5041 ggcgtaccac atccacaggt agctgccctg ctgcgcgatc gcgatgccgc ccagccaggc
     5101 gccgaagaat ccgccgatct ggtgcgacag cagcgtgagg ccgaacagcg tcgcgagata
     5161 cctgacgccg aacagcttgg caaccacgcc tgccgtcggc ggcacagtgg cgagccacgt
     5221 gaagccgagc ccgatcgcga acagatagaa cgtccactcg gtcttcggcg ccagcagata
     5281 cagcagcacg agcaccgtgc gcgaaccgta catccacgcc aggatgtact tgctgcgata
     5341 gcggttcacg caccagccgg cgaacaggct ccccgcgata ttcgcgagcc cgatcagcgc
     5401 cagcgaccag ctcgccacgg aggccggcag cccgcacaag ctcacctcgc ccggcaggtg
     5461 cgtcacgaga aacgcgatat ggaagccgca ggtgaagaag cccgcatgca gcagcaggta
     5521 gctgcgatcg ccgaacgcgg tgcgcagctg tgcgcgcagt ccgccgtcgg catgccgcgc
     5581 ctcgacgtgc gcgcttggcg cgcgccgcag cgcgcgcgcc accggcagcg ccgccagcgt
     5641 gatcgcggcg agcgaccaca tggcacccat ccagccgaac acgttgatca gcttctgcag
     5701 caccggcgcg aacacgaact gcccgaacga gccgccggca ttgatcacac cggacgcggt
     5761 gccgcgatgt tcgaccgata cccgctgcgc ggccgcgccg atcagcaccg agaaacttcc
     5821 cgcgcccgac ccgatcgcgg acaacacccc gagcgagaac accagcccag cgccggtcgt
     5881 catcagcggc gtcagcgcgc tgcccagcgc gaggatcacg agtcccgccg ccagcacgcg
     5941 gccgggccca tagcgatcgg cgatcgcgcc ggcaaccggc tgcaccgcgc cccagacgaa
     6001 ctggccgact gccatcgcga agctgatcgt cacgatgccg agccccgtgc tcgtattcag
     6061 cggcgacacg atcagtccga gcgactggcg cgcgcccatc gtcaccatca gcaacgcggc
     6121 cgccagcaat gtgacgagcc accagttctg cttactactg ctttccatcg cgcccgaatc
     6181 cagtgcgcgc cgccacggcg catccgctcc gattagaacg gtatcggccg cacggcacaa
     6241 atgacaaatg aaattccggc agcgcaggca tgcgcgctgc gcatacctgc gcggcgcgct
     6301 cgactgcacc gaaggccgat ctcggacgac tggctcgact tcaaccgcta cggccgccag
     6361 atcgccgcgc atccgacgcc ggcgcagcgg gccatcgcgc gacgagcgcg gtcgacggcg
     6421 gcgccgtgct ggtgcgtcac ttcggcgcgg cgctgtcgat ccccgagtgg gatcaactcg
     6481 cagccaggct gcacgccgcg ggcgtgcggt tcatcatcga gccccgcctc cgccgcaaag
     6541 gcgagagcgg cgaacaggcg acgatgttct tttgcagtcc ctttggcaat gcgctggagt
     6601 tcaacgcctg cgcggacccg gttcggttgt tcgcgcggtg gtactcagta tttcttcgga
     6661 atctgcggcg tgtcggcggc cgcgtcggga atgcgcgcgg ccgcagccgg cgcgacgtag
     6721 cgcacgcgca ggcgcgcgtc gtcggcgccg ccgacccatg tgtcgacgac gaacagcggc
     6781 ggcgtgctcc ggtcccgggc cgcgggccgc agcatgaagc tgaagatcga tgtcgtgccg
     6841 atctcgtgca ccgccatctg ttcgatcgtg tacgcgcccg tcgcctccgt gccgacccgc
     6901 tgcaggaaat cggcgcgcgg caccggcacg cccggccgcg ccgcgacacg aagctcgaag
     6961 tcgtccgcca gcatccgctc cagcgcagcc gcatcgcgcg cgcgcatcgc cgcaagcatc
     7021 tgatcctcgc gttcgaggta gagcttcacg agccgcgtca cggtgggtat ggggtcggat
     7081 tgccgcgcgg ggaccggcgg cgccagagcc agcagcacgg cgcccagcgc aacggcggtc
     7141 gaacggatca acgactgcat tcggctttga aagcgaacgg ccgccggggt tctgccggcg
     7201 gccgcgcgca tcacatcgtt acggatatgc ggtcgcgcaa taaccgccgc cggcattgac
     7261 ccagttccgc tggatcaggt actggcggct gccgagcgtc atgttgtatt tcgacccgtt
     7321 cgacgctgtc gacgtggtcc cgaacgtcca ggcgcacttg tccgcattct cgtagccgcg
     7381 gcggtcgtac caggcgttca ggttcgg
//
LOCUS       DBNHBY010000551         2763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1120901, whole genome shotgun sequence.
ACCESSION   DBNHBY010000551 DBNHBY010000000
VERSION     DBNHBY010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1120901"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="AC16HB_13540"
     CDS             <1..1358
                     /locus_tag="AC16HB_13540"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR5607856.1"
                     /translation="ALLLSSCGGGGSGTTPVNPQSSNATVQINLGDAPADRLLAAGVT
                     VNAFALTGAGATSVTVLNAPRPVEMMQSMGTVMPLAVASVPQGTYTGMTMTFGATTIA
                     YVDPATGQPVQRAVPGPMTVNVAFSPPLVAGAAPMVVNLDMNMAASVAIDASGNAALT
                     PTIAASMNPVVAGGLDPEDGGMHGVTGMVGGINGSAFTLATMQGMTGMSLQTGSGTQF
                     AGMSGMGMMGNGMLVTLDATLQPDGSWMASRMQSQVGAGGAMAAGLVTNVTGNPPTQL
                     TLVMQDGAGNGMMAANIAGTTTVNLDSATQFSIDTDGVDLANLPFAPRFDRLSLSQGQ
                     RVRAISAGPMMQGGGMGGMSGGATLAATSVQLGAQALRGTAADYSRSGAQATFTLVLP
                     ADSAFARLAGVTAVTVYQQGATQLRGLSTIADGSTVQVRGLLFLDGGVFRMVAGRIAG
                     A"
     gene            1564..1857
                     /locus_tag="AC16HB_13545"
     CDS             1564..1857
                     /locus_tag="AC16HB_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607857.1"
                     /translation="MAGVDFVYVGGEAVFRLQDVPSAGLTRAEKRAQDTAQVAAAAAQ
                     PAGWRRVSGEPGWILEGHKYARVGGEFTCVDGIDHSVQASFAPFADPTLYRGV"
     gene            1945..2304
                     /locus_tag="AC16HB_13550"
     CDS             1945..2304
                     /locus_tag="AC16HB_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607858.1"
                     /translation="MSFNLTDSSAPRIAIAMLAGLLPLHAFAAALNLRIEAGKFDEHC
                     VKLAAGAEVKYRFKVGTPVDFNIHHHRGNDVFYPVRHEQVRKLAGRFRAPAADDYCLM
                     WENNGAQAVTVRGMAEP"
     gene            2312..>2763
                     /locus_tag="AC16HB_13555"
     CDS             2312..>2763
                     /locus_tag="AC16HB_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR5607859.1"
                     /translation="MAAPRETELKLAVAAADLGRLRRRLRRFGRPTIEPFETVYYDSA
                     DFTLARQGIALRVRRAGRRWLQTLKTLGAQSALARRGEWEFAAPRGKLDLARFAGTPL
                     STLLRAEPALAIEPRFRTRFTRALWTTADRAIEIALDQGEVVAGERTTP"
BASE COUNT          446 a          923 c          958 g          436 t
ORIGIN      
        1 cggccctgct cttgtcgtcc tgcggtggcg gcggctccgg aacgacgcct gtcaatccgc
       61 agtcgtcgaa tgcgacggtg cagatcaatc tcggcgacgc gcccgcggat cgcctgctcg
      121 ccgccggagt gacggtcaac gcattcgcgt tgacgggtgc gggcgccacc tccgtgaccg
      181 tgctgaacgc gccgcggccg gtggagatga tgcagtcgat gggcaccgtc atgccgctcg
      241 cggtcgccag cgtgccgcag ggaacatata cgggcatgac gatgaccttc ggcgccacga
      301 cgatcgctta tgtcgatccg gcgacaggcc agccggtgca gcgcgccgtg ccggggccga
      361 tgacggtgaa cgtggcgttc agcccgccgc tcgttgccgg tgctgcgccg atggtcgtga
      421 atctcgacat gaacatggcg gcatcggtcg cgatcgatgc gagcggcaat gccgcactga
      481 cgccgaccat tgccgcgtcg atgaatcccg tcgtcgctgg cggcctcgat cccgaagacg
      541 gcggcatgca cggtgtgacc ggcatggtgg gcggcatcaa cggcagtgcg ttcacactgg
      601 caacgatgca aggcatgacc ggtatgtcgc tgcagaccgg cagcggcacg caattcgcgg
      661 gcatgagcgg catgggcatg atgggcaacg gcatgctggt tacgctcgat gcgacgctgc
      721 agcccgacgg tagctggatg gcaagccgca tgcagtcgca ggtaggcgcc ggcggcgcaa
      781 tggccgccgg gctggtcacg aacgtcaccg gcaatccgcc gacgcagttg acactggtga
      841 tgcaggacgg tgccggcaac ggcatgatgg ctgcgaacat cgccggcacg acaaccgtca
      901 atctcgacag cgcaacgcag ttctcgatcg acactgacgg tgtcgacctc gccaacctgc
      961 cgtttgcgcc gcgcttcgac cggctctcgt tgagccaggg ccagcgcgtg cgtgcaatca
     1021 gtgccggacc gatgatgcag ggcggcggca tgggcggaat gagcggcggt gcgacgcttg
     1081 ccgccacatc ggttcagctc ggtgcccagg cgctacgcgg caccgctgcc gactacagcc
     1141 gcagcggcgc gcaggcgaca ttcacgctcg tgctgccggc cgattccgca ttcgcgcggc
     1201 tcgccggcgt cactgcggtc acggtgtatc agcaaggcgc cacgcagctg cgcggactgt
     1261 cgacgatcgc cgacggcagc acggtgcagg tacgtggact gctgttcctc gacggtggcg
     1321 tgttcaggat ggtagccggc cgcattgccg gcgcgtgatg cgacaccgga tctatcaatc
     1381 gagcgttgcc gatttgtaat tgcgtggtca gcgcttcgtc gccaccgcgc ggccacactg
     1441 ggcaccgtgc aagtcagacg ctgcacgcaa ccctggcaac aacactcgat ggaggatttc
     1501 atgtcaacac gcaaactgct cctcgccctt gcattcgcga gcgccgcagt gccgctttcc
     1561 gcgatggccg gcgtcgactt cgtctacgtc ggcggtgaag cagtctttag attgcaggac
     1621 gttccgagcg ctggtctgac gcgcgccgaa aagcgcgcac aagacacggc gcaggtcgct
     1681 gccgccgctg cgcaaccggc cggctggcgc cgggtcagcg gcgagcccgg ctggattctt
     1741 gaagggcaca agtacgcgcg cgtcggcggc gagttcacgt gtgtcgacgg tatcgatcac
     1801 agcgttcagg caagcttcgc gccgttcgcc gacccgacgc tctaccgagg ggtgtagcga
     1861 cacgcgcgac gcgggcgaag cgcgcggtag ccggtcgccg gtaccattcg cgctccggcc
     1921 accaagagct tcgcgtacgc gtcgatgtcg ttcaacctga ccgatagctc agccccgcgc
     1981 atcgcaatcg cgatgctggc ggggctgctg ccgctgcacg cattcgccgc cgcgctcaac
     2041 ctgcggatcg aggccggcaa gttcgacgag cattgcgtga aactcgccgc cggcgcagag
     2101 gtgaagtacc gcttcaaggt cggcacgccg gtcgacttca atatccatca tcatcgcggc
     2161 aacgacgtgt tctacccggt ccggcacgag caggtgcgca agctcgcggg gcgctttcgc
     2221 gcgccggccg ccgacgacta ttgcctgatg tgggagaaca acggcgcgca ggcggtcacc
     2281 gtgcgcggca tggcggagcc gtagccgcgc gatggctgcg ccgcgcgaga cggaactgaa
     2341 gctcgcggtc gccgcggccg atctcgggcg gctgcggcgg cgcctgcggc gcttcggccg
     2401 acccacgatc gagccgttcg agaccgtcta ctacgacagc gccgatttca cactcgcgcg
     2461 gcagggcatc gcattgcgcg tgcgccgcgc gggacggcgc tggctgcaga cgctcaagac
     2521 gctcggcgcg cagtccgcgc tcgcgcgccg tggcgaatgg gagttcgcag cgccgcgcgg
     2581 gaagctcgat ctggcgcgct ttgccggcac gccgctctcg acgctgttgc gtgcggagcc
     2641 ggcgctcgcg atcgagccgc gcttccgcac gcgcttcacg cgcgcgctgt ggaccacggc
     2701 cgatcgcgcg atcgagatcg cgctcgacca gggcgaagtc gtcgccggcg aaaggacgac
     2761 gcc
//
LOCUS       DBNHBY010000552         1910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_645663, whole genome shotgun sequence.
ACCESSION   DBNHBY010000552 DBNHBY010000000
VERSION     DBNHBY010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_645663"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC16HB_13560"
     CDS             complement(<1..319)
                     /locus_tag="AC16HB_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607860.1"
                     /translation="MFGPITVPFGGKMLFNTLKLKPGVTYDDVELALAEMCNVVKETY
                     GGDKGGFIAGQVFKFSGFLSAEGSLNNNRTADHDMVIVTYWRSFEEHERSHADRTFKE
                     KFAA"
     gene            449..922
                     /locus_tag="AC16HB_13565"
     CDS             449..922
                     /locus_tag="AC16HB_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607861.1"
                     /translation="MLRTIAVALAMLSAPALAADPSPVVTYSKAAKFDDVRDDLKAAI
                     ESKGLVIDYQSHINRMLERTGKDVGSARKVYTDAQAFVFCSARLSRKTMEADPANVSM
                     CPYTMVVYATAQEPQKVAVTYRRPWRPDGSAASKAALKEVESLLDGIAREALGIK"
     gene            1167..>1910
                     /locus_tag="AC16HB_13570"
     CDS             1167..>1910
                     /locus_tag="AC16HB_13570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5607862.1"
                     /translation="MIPAGFDYHAPRSVADAVKMLGDLGPDAKLLAGGHSLLPMMKLR
                     FAAPSHLIDLGRIPELRGIAQVGNEIRIGAMTTESTLLRSTLLAEKVPLLVEGVSTIA
                     DPQVRYKGTIGGDISHGDPGNDHPALMIALDASFVLSGPKGERVVKADGYFLGLYSTL
                     LEPNEILTQIRIPIPAAGSGWSYQKLKRKAGDFATAATAVLLQMKGGSIAKVAIALTN
                     VGPTALKASAAEAYLSGKTINEASLNEAAR"
BASE COUNT          353 a          616 c          634 g          307 t
ORIGIN      
        1 gcgcggcgaa tttctccttg aacgtcctgt cggcgtgcga acgctcgtgc tcctcgaacg
       61 accgccagta cgtgacgatc accatgtcgt gatcggccgt gcggttgttg ttcagcgagc
      121 cttccgcgga caggaagccc gagaacttga acacctggcc ggcgatgaaa ccgcccttgt
      181 cgccaccgta ggtctccttc accacgttgc acatctcggc gagcgccagt tcgacgtcgt
      241 cgtaggtgac gccgggtttg agcttcagcg tgttgaacag catcttgccg ccgaacggaa
      301 cggtgatcgg accgaacatc gttgcctcct tggatcgttg gagcgccgga catggggccg
      361 gcacgctgca cttcaagcgg tgagcgtcgc ataaacggga ccgccaagcc cacaacgaag
      421 ttacggacat atcgacaggg aggtcaaaat gcttcgcacc atcgccgtcg cgctcgccat
      481 gctgagcgca cccgcactcg ccgcagatcc gtcgccggtg gtcacttact cgaaggcggc
      541 gaagttcgac gacgtccgcg acgacctgaa ggccgcgatc gaaagcaagg gcctggtgat
      601 cgactaccag tcgcacatca accgcatgct cgagcgcacc ggcaaggacg tgggatcggc
      661 gcgcaaggtg tacacggacg cgcaagcgtt cgtgttctgc agcgcgcggc tctcgcgcaa
      721 gacgatggag gccgatccgg ccaacgtgtc gatgtgtccg tacacgatgg tcgtgtacgc
      781 gaccgcgcag gagccgcaga aggtcgcggt gacgtaccgc cggccatggc ggccggacgg
      841 atcggccgca tcgaaagcag cgctgaagga agtggagtcg ctgctcgacg gcatcgcgcg
      901 cgaagcgctg ggaatcaagt agcttccggc gcgcacccgg ctgcaagttc gttcgcggcg
      961 tcgcgcgctt gggtattgcc gaagcgggcg gcgaagacta aggttttccg cccggcgccc
     1021 tgcgcgcgac atcgtgcggt tcgtgttcgg caccgtgcac ttcgtgcacg gttgcgaccg
     1081 gcggcactgc tgcgcggcta tggtcggccg gggcgacaga agcggagacg tcaggtccat
     1141 ccgccctcct cgaggagaca agcacgatga ttcccgcggg attcgactac cacgcgccac
     1201 gttcggtggc ggacgccgtg aagatgctcg gcgatctcgg tcctgacgcc aagctgctgg
     1261 cgggcgggca cagcctgctg ccgatgatga aactgcgatt cgcggcgcca agccacctaa
     1321 tcgatctggg ccggattccc gaattgcgcg gaatcgcgca ggtcggcaac gagatccgta
     1381 tcggcgccat gaccaccgaa agcaccctgc tgcgctcgac gctgctcgcg gagaaggtgc
     1441 cgctgctggt cgagggagtg agcacgatcg ccgacccgca ggtgcgctac aagggcacga
     1501 tcggcggcga catttcgcat ggcgacccgg gcaacgatca cccggcgctg atgatcgcgc
     1561 tcgacgcctc gttcgtgttg agcgggccga agggcgagcg cgtggtgaag gccgacggct
     1621 actttctcgg cctctactcc accctgctgg agcccaacga aatcctgacg cagatccgca
     1681 taccgatccc ggccgcgggc agcggttggt cgtaccagaa gctcaagcgc aaggccggcg
     1741 actttgccac cgcggcaacc gcggtcctgc tgcagatgaa gggcggcagc attgccaagg
     1801 tcgccatcgc gctcaccaac gtcggcccga ccgcgctcaa ggccagtgcc gcggaggcct
     1861 acctttcggg caagacgatc aacgaggcca gcctgaacga ggcggcgcgg
//
LOCUS       DBNHBY010000553         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_713521, whole genome shotgun sequence.
ACCESSION   DBNHBY010000553 DBNHBY010000000
VERSION     DBNHBY010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_713521"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC16HB_13575"
     CDS             complement(<1..617)
                     /locus_tag="AC16HB_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR5607863.1"
                     /translation="MSEPDRTHDSNPAPPKPTRALVFGASGYIGTHLVQRLRTEGWSV
                     RACARTRKVLEARAWDAVELVEADALKPATLATALAGVDVAYYLVHSMAAGRDFGRLD
                     LEAADNFARAAAAAGVKRIVYLGGLVPSGADSEHLQSRAQTGARLRAGSVPITEIRAG
                     IIVGPGSAAYEVMRDLVNHLPVMVTPRWVRSKSSPIALANLLEYLV"
     gene            742..2637
                     /locus_tag="AC16HB_13580"
     CDS             742..2637
                     /locus_tag="AC16HB_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR5607864.1"
                     /translation="MSTVASHDQSRAHGSRFPLLVVGAIGVVFGDIGTSPLYALKEVF
                     GSQHPIPLTEANVLGALSLVFWTLMVIVTLKYVTLILRADNHGEGGILALLALVVRLT
                     RNQKRLRYVLGLLGVFGATLFYGDAVITPAISVLSAVEGLEVIAPRLHPFIVPTALTV
                     LVALFLIQSYGTGKVGAFFGPVTLAWFVTLAVLGIASIAQTPDVLVALHPVHSYRFFA
                     YHPLISFIALGAVVLAVTGTEALYADMGHFGRGPIRVAWLFVVWPALLANYFGQGALL
                     LREPKAAENPFYLLAPDWFLVPLVVLATAATVIASQAVISGAFSLTRQAIQLGLLPRM
                     AIRHTSAREEGQIYIGLVNWLLLAGVVFLVLEFRSSSGLAAAYGMAVTGAMMVDTLLV
                     GAVAVLMWHWPRPLAAVMVGTLLAIDFTFFAANTLKFLSGGWLPLAIALALFTVLTTW
                     RHGRRLLIARKRENAIGRDDFIKLMADKIPRVPGTAVFMTGDREIAPSALLHNLKHNK
                     VLHERVIFLTVKVEAEPYVPEEKRVDIKDLGKNFYRVITRYGFMQQPDIPAALEQAAR
                     AGLPFEMMETTFVLGRETVIPTKLAGMAIWREHIFAWMMRNATSANDFFRIPANRVVE
                     LGSQIEI"
     gene            2640..3191
                     /locus_tag="AC16HB_13585"
     CDS             2640..3191
                     /locus_tag="AC16HB_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR5607865.1"
                     /translation="MEALFVSTALVALAEMGDKTQLLSFVLAAKLKKPWPIILGIFFA
                     TLANHFLAGWVGAWLATLVAPATMKWIIAISFFVFGAWALVPDKFDATREYSGRSVFV
                     TTLIAFFLVEMGDKTQLATVALAAKYPSLVAVVMGTTLGMLIANAPAVWLGEALAHRV
                     NMKVMRWIAAGLFAVLGALTLML"
     gene            3280..>4094
                     /locus_tag="AC16HB_13590"
     CDS             3280..>4094
                     /locus_tag="AC16HB_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607866.1"
                     /translation="MSPVGISLAFVVAFGLFVWLAWRKLAIVAALQPESRWDAPGERI
                     RQVLVNGFLQSRMVRGDFRPGVMHAVIFAGFMILLVRKVQLLVIGWYEPFTYPGAFGA
                     GFAWLKDLVELAVLAAVSYGFWRRLVHPARRLEPNREALAILGLITAIMLTDFAFDGF
                     RFALLTDAHPAIAHERAYAFVGGALATAFSALPPAALTVGYHLSYWLQILTVFAFLVW
                     LPLGEHFHIVTALPALFFARRPPGPLAVVPAVNLEPLMNEGGDEEIRVGIRTA"
BASE COUNT          553 a         1331 c         1437 g          773 t
ORIGIN      
        1 accaggtact cgagcaggtt ggcgagcgcg atcgggctcg acttcgaccg cacccagcgc
       61 ggcgtgacca tcaccgggag gtggttgacg aggtcgcgca tcacctcgta cgccgccgag
      121 cccgggccga cgatgatgcc ggcgcggatc tcggtgatcg gcacgctgcc cgcgcgcagt
      181 cgcgctccgg tctgtgcgcg cgattgcaga tgctcggagt cggcaccgga cggcacgagc
      241 ccgcccaggt acacgatgcg cttgacaccg gccgcggcgg ccgcgcgcgc gaagttgtcg
      301 gcggcctcga gatcgagccg gccgaagtcg cggcccgccg ccatcgagtg cacgaggtag
      361 tacgcgacgt cgacgcccgc gagcgcggtc gcaagcgtgg cgggcttcag cgcgtcggct
      421 tcgaccagtt cgaccgcgtc ccatgcgcgc gcttcgagca ccttgcgcgt gcgcgcacag
      481 gcacgtaccg accagccctc ggtgcgcagt cgctgcacga ggtgggtgcc gatgtagccg
      541 ctggcgccga acacgagcgc acgcgtcggc tttggcggcg cggggttcga atcgtgggtg
      601 cggtcaggtt cgctcaagtt gtcggctgga agcgacggtt gttggaatgc ccggttcact
      661 tgcgcgagcg tagcgtgagg catcggcgta cagtcgcgcc gggttttgct gcccgccgaa
      721 cgggaaacgg tcaagaaagc aatgtccacg gtggcttcgc acgaccagtc gcgggcgcac
      781 ggttcgcgct ttcccctgct cgtggtcggc gcgatcggtg tggtgttcgg cgacatcggc
      841 accagcccgc tgtacgcgct gaaggaggtg ttcgggtcgc agcacccgat cccgctcacc
      901 gaggccaacg tcctcggcgc gctgtcgctg gtgttctgga cgctgatggt gatcgtcacg
      961 ctcaagtacg tgaccctgat cctgcgcgcg gacaaccacg gcgagggcgg catcctcgcg
     1021 ctgctggcgc tggtggtgcg cctcacgcgc aaccagaagc ggctgcgcta cgtgctcgga
     1081 ctgctcggcg tcttcggcgc gacgctcttc tacggcgacg ccgtgatcac gcccgcgatt
     1141 tcggtgctgt ccgcggtgga ggggctcgag gtcatcgcgc cgcggctgca tccgttcatc
     1201 gtgccgaccg cgctgacggt cctggtcgcc ctgttcctga tccagagtta cggcaccggc
     1261 aaggtcggcg cgttcttcgg cccggtcacg ctggcgtggt tcgtcacgct cgcggtgctg
     1321 ggcatcgcca gcatcgcgca gacgccggac gtgctggttg cactgcatcc ggtgcactcg
     1381 tatcgcttct ttgcctacca cccgctgatt tcattcattg cgctgggcgc ggtcgtgctc
     1441 gcggtgaccg gcaccgaggc actgtatgcg gacatgggcc acttcggccg cgggccgatc
     1501 cgtgtcgcgt ggttgttcgt cgtctggccg gcgctgctgg ccaattattt cggtcaggga
     1561 gcgttgctgc tgcgcgagcc caaggcggcc gagaacccct tctacctgct cgcaccggac
     1621 tggttcctcg taccgttggt ggtgctggcg accgcggcca ccgtcatcgc gtcgcaggcg
     1681 gtgatctccg gtgccttctc gctgacgcgg caggcgatcc agctcggcct gttgccgcgc
     1741 atggcgatcc ggcacacgtc ggcccgcgag gaaggacaga tctacatcgg gctcgtgaac
     1801 tggctgctgc tggccggcgt ggttttcctg gtattggaat tccgctcgtc gtcggggctg
     1861 gcggccgcgt atggcatggc ggtcaccggc gcgatgatgg tcgacacgct gctggtgggc
     1921 gccgtggcgg tgctgatgtg gcactggccg cggccgctgg cggcggtgat ggtcgggacg
     1981 ctgctggcga tcgacttcac gttcttcgcc gccaacacgc tgaagttcct ctccggcggc
     2041 tggctgccgc tggcgatcgc gctggcgctg ttcacggtgc tgacgacgtg gcggcacgga
     2101 cgtcgcctgc tgatcgcgcg caagcgggag aacgcgatcg gccgcgacga cttcatcaaa
     2161 ctgatggccg acaagattcc gcgcgtgccc ggcaccgcgg tgttcatgac cggcgaccgc
     2221 gagatcgcgc ccagcgcgct gctgcacaac ctgaagcaca acaaggtgct gcacgagcgc
     2281 gtgatcttcc tgaccgttaa ggtcgaggcg gagccgtatg ttccggagga gaagcgcgtc
     2341 gacatcaagg acctcggcaa gaacttctac cgtgtcatca cgcgctacgg cttcatgcag
     2401 cagcccgaca tcccggcggc gctcgaacag gcggcgcggg cggggctgcc gttcgagatg
     2461 atggagacca cgttcgtgct cggccgcgag accgtgatcc cgaccaagct ggccggcatg
     2521 gcgatctggc gcgagcacat ctttgcctgg atgatgcgca acgcgaccag cgccaacgac
     2581 ttcttccgca tccccgccaa ccgagtggtc gaactcgggt cgcagatcga gatctagcgt
     2641 tggaggcact gttcgtttcc accgcgctgg tcgcgctggc cgagatgggc gacaagacgc
     2701 agctgctgtc gttcgtgctg gcggcgaagc tgaagaagcc gtggccgatc atcctcggca
     2761 tcttctttgc cacgctggcc aatcacttcc tcgccggctg ggtcggcgct tggctggcga
     2821 ccctggttgc gcctgcgacg atgaagtgga tcatcgcgat ctcgttcttc gtgttcggtg
     2881 cctgggcgct ggtgcccgac aagttcgacg cgacgcgcga atactcgggc cgcagtgtct
     2941 tcgtcaccac gctgatcgcg ttctttttgg tcgagatggg cgacaagacg cagctggcca
     3001 ccgtcgcgct ggcggcaaag tatccgtcgc tggttgcagt ggtcatgggc accacgctcg
     3061 gcatgctgat cgccaacgcg ccagcggtgt ggctcgggga ggcgctcgcg catcgggtca
     3121 acatgaaggt gatgcgctgg atcgcggcgg ggttattcgc ggtgctcggc gcactgacgc
     3181 tgatgctttg agcgccggga ttgcgcacgg ttgagttagc gcaagccggg ccgttgcgcc
     3241 cgacggtacg gtgtggcgtc ccgatttgcg tcattcgcga tgagtccggt cggcatatcg
     3301 ctcgcgttcg ttgtcgcctt cggcctgttc gtctggctcg cgtggcgcaa gctcgcgatc
     3361 gtggcggcgc tgcagcccga gtcgcgctgg gatgcgccgg gcgagcgcat ccggcaggtt
     3421 ctggtcaacg gtttcctgca gtcgcgcatg gtgcgcggcg actttcgccc cggcgtcatg
     3481 cacgcggtca tcttcgccgg cttcatgatc ctgctggtgc gcaaggtgca gttgctggtg
     3541 atcggctggt acgagccgtt cacctatccg ggcgcgttcg gcgccggctt cgcgtggctg
     3601 aaggatctgg tcgagctcgc ggtgctcgcc gcggtgagtt acggcttctg gcggcggctg
     3661 gttcacccgg cgcgccggct cgagccgaac cgggaggcgc tggcgatcct gggactcatc
     3721 accgcgatca tgctgaccga cttcgcgttc gacggcttcc gcttcgcact gctgaccgat
     3781 gcacacccgg cgatcgcgca cgagcgcgcc tacgcattcg tcggcggcgc gctggcgacc
     3841 gcgttctccg cgctgccgcc ggcggcgctg acggtcggct atcacctgtc gtactggcta
     3901 cagatactga ccgtgttcgc gttcctggtg tggctgccgc tgggcgagca cttccacatc
     3961 gtcaccgcgc tgccggcgct gttctttgcg cggcgtccgc cggggccgct cgcggtcgtg
     4021 ccggcggtga acctcgagcc gctgatgaac gagggcggcg acgaggagat ccgggtcggc
     4081 atccgcaccg ccaa
//
LOCUS       DBNHBY010000554         2183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1186915, whole genome shotgun sequence.
ACCESSION   DBNHBY010000554 DBNHBY010000000
VERSION     DBNHBY010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1186915"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="AC16HB_13595"
     CDS             complement(<1..625)
                     /locus_tag="AC16HB_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607867.1"
                     /translation="MAELLDVERLTAGYGAGVVLEDVSFALEEGDSLALLGRNGMGKT
                     TLLVTLMGLTQRHRGALRWMGADLAVLPTHRRCRAGLGWVPQERFMFPSLTVEEHLTV
                     VARPGRWTVARIYELFPRLAERRANYGNQLSGGEQQMLAIARALMVNPRLLLLDEPME
                     GLAPIIVQELMGVIRELIEEGGMAIIIVEQHARLALALTRQAIVLERG"
     gene            complement(618..1370)
                     /locus_tag="AC16HB_13600"
     CDS             complement(618..1370)
                     /locus_tag="AC16HB_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607868.1"
                     /translation="MTPALRTAGLSKHFGAFRANSNITLAFPQGARHALIGPNGAGKT
                     TLINLLTGALAPTAGDVFLGHERITALPQHLRVKRGMTRTFQINTLFANLTVHESVTL
                     AVCERNGRAGIWHRTVASQREEADEALALLQSLQLARDADTLTRNLPYGKQRLVEIAL
                     ALATRPRILLLDEPAAGIPAGASAELFGVIAALPRDVTILFIEHDMDLVFRFAERITV
                     LVAGTVLTEGAPAEIAADPRVREVYLGEAQHG"
     gene            complement(1367..>2183)
                     /locus_tag="AC16HB_13605"
     CDS             complement(1367..>2183)
                     /locus_tag="AC16HB_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5607869.1"
                     /translation="ILGYAGIVSLGHAAYFGIGAYAAGLLAVHGWGEPLSGLAVAGFA
                     SAILGFATSFLVVRGSDLARLMVTLGIGLMLFEAANQLPSITGGADGLTGVTMWKLLG
                     AFSFDLRGSTAYVYSLVVLFLLFVVARRIVASPFGLSLRGVRENVRRMPAIGAPVGRR
                     LVAVFTISAAMAGVAGGLLAQTTQFVGLDVFGFPRSADLMIMLVLGGAGRLYGGLLGA
                     ALFMVAHHFLSDLNPVYWQFWLGLLLVVVVLFARGGILGAAAALRERMRGGRR"
BASE COUNT          372 a          777 c          737 g          297 t
ORIGIN      
        1 ggccgcgctc gagaacgatc gcctggcgcg tcagcgccag cgccagtctg gcgtgctgct
       61 cgacgatgat gatggccatg ccgccctcct cgatcagttc acgaatgact cccatcaact
      121 cctgcacgat gatcggcgcc agcccctcca tcggctcgtc gagcaggagc aggcgcggat
      181 tgaccatcag cgcgcgcgcg atcgcgagca tctgctgttc gccgccggaa agctggttgc
      241 cgtagttcgc ccggcgctcg gccaggcgcg gaaagagttc gtagatcctg gcgacggtcc
      301 agcgacccgg ccgcgcgacg acggtcaggt gctcctcgac cgtcagcgac gggaacatga
      361 accgctcctg cggcacccag ccgagccccg cccggcaccg ccgatgcgtg ggcaggacgg
      421 cgaggtcggc gcccatccag cgcagggcgc cgcgatgcct ctgtgtcagc cccatcagcg
      481 tcacgagcag cgtggtcttg cccatgccgt tgcggccgag cagcgcgagg ctgtcgccct
      541 cttccagcgc gaacgacacg tcctccagca cgacgccagc gccataaccc gcggtgaggc
      601 gctcgacatc gagcagttca gccatgctgc gcctcgccga ggtacacctc gcgcacgcgt
      661 gggtcggccg cgatctcggc cggtgcgccc tcggtcagca ccgtacctgc gaccagcacg
      721 gtgatgcgtt cggcgaacct gaacacgagg tccatgtcgt gctcgatgaa caggatcgtg
      781 acgtccctgg gcagcgcggc gatcacgccg aacagttccg cgctcgcgcc ggccgggatg
      841 ccggccgccg gttcgtcgag cagcaggatg cgcggccggg tcgcgagcgc gagcgcgatc
      901 tcgaccagtc gctgtttacc gtacggaagg ttgcgcgtca gcgtgtccgc gtcgcgcgcc
      961 agttgcaggc tttgcagcag cgcaagggcc tcgtccgcct cctcgcgctg gcttgcgacc
     1021 gtgcgatgcc agatgccggc gcgcccgttt ctctcgcaca ccgccagcgt cacggattcg
     1081 tgcaccgtga ggttggcgaa cagcgtgttg atctggaacg tgcgcgtcat gccccgcttc
     1141 acgcgcaggt gctgcggcag cgcggtgatg cgctcgtggc cgaggaacac gtcgcccgca
     1201 gtcggggcca gcgcaccggt gagcaggttg atcaacgtgg tcttgccggc gccgttcggg
     1261 ccgatcagcg catgccgcgc cccctgcggg aatgcgagcg tgatgttgct gttggcgcga
     1321 aacgcgccga agtgtttcga aagccccgcg gtgcggagcg cgggagtcat cgtcggcctc
     1381 cgcgcatccg ctcgcgcagc gccgcggccg cgccgagaat tccgccgcgc gcgaacagca
     1441 cgacgaccac cagcagcagg ccgagccaga actgccagta gaccgggttg aggtcggaga
     1501 ggaagtggtg cgcgaccatg aacagcgcgg cgccgagcag cccgccgtac agtcgacccg
     1561 cgccgccgag caccagcatg atcatcaggt ccgctgatcg cggaaagccg aatacgtcca
     1621 gacccacgaa ctgcgtcgtc tgcgcgagca acccccctgc cacaccggcc atcgctgcgc
     1681 tgatcgtgaa gaccgcgacg aggcgacggc cgaccggcgc gccgatggcg ggcatgcggc
     1741 gcacgttctc gcgcacgccg cgcagcgaca gcccgaaggg ggaggcgacg atgcggcgtg
     1801 cgacgacgaa cagcaggaag agcacgacga ggctgtagac atacgccgtg ctgccgcgca
     1861 gatcgaagga aaacgcaccc agcagcttcc acatcgtcac gcccgtgagc ccgtcggcgc
     1921 cgccagtgat cgagggcaac tggttggcgg cctcgaacag catcaagccg atgcccagcg
     1981 tcaccatcag gcgcgccagg tccgagccgc gcaccaccag aaagctcgtt gcgaagccca
     2041 ggattgcgct ggcgaaccca gcgaccgcga gcccgctcag cggttcgccc cagccgtgca
     2101 ccgcgagcag gcccgccgcg taggcaccga tgccgaagta ggccgcatgc ccgagcgaca
     2161 cgatgccggc gtagcccagg atc
//
LOCUS       DBNHBY010000555         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1795817, whole genome shotgun sequence.
ACCESSION   DBNHBY010000555 DBNHBY010000000
VERSION     DBNHBY010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1795817"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16HB_13610"
     CDS             <1..180
                     /locus_tag="AC16HB_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607870.1"
                     /translation="LGLGAPPSVPSLGDLLRQGKANLDAWWIIVPTFVLLVVTMALLT
                     FIGDALRDAFDTRKS"
     gene            177..>1565
                     /locus_tag="AC16HB_13615"
     CDS             177..>1565
                     /locus_tag="AC16HB_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607871.1"
                     /translation="MSGELLSVERLRVNFGDACVVDDVSFKIAAGEKFALVGESGSGK
                     SITALSILRLVEGATTTGAIRFDGDDVVGKSERAMRGLRGRDIAMIFQEPMTALNPLY
                     TVGNQIGEVLELHEGMRPNAARARAIELLAKTGIPEPARRIDAYPHQLSGGQRQRAMI
                     AMALACRPKLLIADEPTTALDVTIQAQILALLDELQREMGMALLFITHDLNLVRRFTH
                     RVGVMEKGRLVEQGVTAEVFGAPRHPYTVKLIASRPQRAVRPLAANAPRLVDLNQVDV
                     QFTSRRGWFGRHLFEAVRHATLELARGETLGIVGESGSGKTTLGMALLALQPIAGGAV
                     HLGDVRVDNADAATLRALRRRMQVVFQDPFASLSPRMTIERIVGEGLELHQPELSREQ
                     RCDRIVAMLAEVGLREQDGLDNLLNRYPHEFSGGQRQRIAIARAVILRPEVLVLDEPT
                     SALDVSVQQQVLK"
BASE COUNT          244 a          523 c          566 g          232 t
ORIGIN      
        1 ctcgggctgg gcgcgccgcc gtcggtgccg tcgctcggcg acctgctgcg ccagggcaag
       61 gccaatctgg acgcgtggtg gatcatcgtg ccgaccttcg tgctgctggt ggtcacgatg
      121 gcgctgctca ccttcatcgg cgacgcgctg cgcgatgcgt tcgacacgag gaaatcgtga
      181 gcggcgaact gctgtcggtc gaacgactgc gcgtgaactt cggcgatgcg tgcgtggtcg
      241 atgatgtgtc gttcaagatc gcggccggcg aaaagttcgc gctggtcggc gaatccggct
      301 ccggcaagtc gatcaccgcg ctgtcgatcc tgcgcctggt cgaaggggcg accacgaccg
      361 gcgcgatccg cttcgacggc gacgacgtcg tcggcaagag cgagcgcgca atgcgcgggc
      421 tgcgcggccg cgacatcgcg atgatcttcc aggagccgat gacggcgctg aacccgctgt
      481 acacggtcgg caaccagatc ggcgaggtgc tcgaactgca cgagggcatg cgccccaacg
      541 cggcgcgcgc ccgcgcgatc gaactgctgg cgaagaccgg cattcccgag cctgcgcgcc
      601 gcatcgacgc ctatccgcat cagctgtcgg gcgggcagcg gcagcgcgcg atgatcgcga
      661 tggcgctcgc atgcaggccg aagctgctga tcgccgacga gccgaccacc gcgctcgacg
      721 tcacgatcca ggcacagatc ctcgcgctgc tcgacgaact gcagcgcgag atggggatgg
      781 cgctgctctt catcacgcac gacctgaacc tagtgcgccg cttcacgcac cgcgtcggcg
      841 tgatggagaa gggccggctc gtcgagcagg gcgtcaccgc cgaagtgttc ggcgcgccgc
      901 ggcatcccta cacggtcaaa ctgatcgcga gcagaccgca gcgcgcggtg cggccgctcg
      961 cggcaaacgc gccgcggctg gtcgacctga accaggtgga cgtgcagttc acgagccggc
     1021 gcggctggtt cggccggcac ctgttcgagg cggtgcgcca cgcgacgctg gaactcgcgc
     1081 gcggcgagac gctcggcatc gtcggcgaat cgggctcggg caagaccacg ctcggcatgg
     1141 cgctgctcgc gctgcagccg atcgcgggcg gcgcggtgca tctgggcgat gtgcgcgtcg
     1201 acaacgcgga cgccgccacg ctgcgcgcgc tgcggcggcg catgcaggtc gtgttccagg
     1261 atccgttcgc gtcgctgtcg ccgcgtatga cgatcgagcg gatcgtcggc gaggggctcg
     1321 aactgcacca gcccgaactg tcgcgcgagc agcgatgcga ccgcatcgtc gcgatgctgg
     1381 ccgaggtcgg gctgcgcgag caggacggcc tcgacaatct gctgaaccgc tatccgcacg
     1441 agttctccgg cggccagcgg cagcggatcg cgatcgcgcg cgcggtgatc ctgagacccg
     1501 aggtgctggt gctggacgaa ccgacctccg cgctcgacgt gtcggtgcag cagcaggtgc
     1561 tgaag
//
LOCUS       DBNHBY010000556         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_308302, whole genome shotgun sequence.
ACCESSION   DBNHBY010000556 DBNHBY010000000
VERSION     DBNHBY010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_308302"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16HB_13620"
     CDS             complement(<1..274)
                     /locus_tag="AC16HB_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HIR5607872.1"
                     /translation="MLPALTALALVLPAHAAELKPGDFGARFGLATQGAGPHFRAPLT
                     ADVVRYSQTPDLADLRVFNAAGEALPFALTSPSVAEPPRETPQPLPV"
     gene            complement(277..>1850)
                     /locus_tag="AC16HB_13625"
     CDS             complement(277..>1850)
                     /locus_tag="AC16HB_13625"
                     /inference="COORDINATES: protein motif:HMM:NF021606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HIR5607873.1"
                     /translation="RRLERLRLLVECFIALGVIFASLAIPFALDARWTAAAWALEGAG
                     VFWVGARQGRRLARAFGLLLQLGAAMAFLRHLGFRPPQGWPLANADFIGAVLLAGTAA
                     FCGRSISERANRYVKGHGWDEFERALGAPLALYGFAWWMAAIVLELTRRYYGGPGTLE
                     FAVEPLDRLFPLMAAYVLSASVACHAGLNRPWRVATWPAYASLPAMLLVAVFGVLIYR
                     HDFEHLGWICWPLAIAAHLFTLRTLDAALKRWWVAVHAGGVYLLVLLAGSLGVDLIDR
                     AELWNTSWGPAASLAALAAVLFAITQAATVPAGRARWPMNRYARAYGWLGLLPVAGFA
                     VVGALLSALTQRGDATPLPHVPLLNPTDLSVLLALAAAWQWRARIAATDWPLPAALRS
                     EPVWIATIGAALFVWLNSVWLRIAHHYFGVEWDAAGLFESFVVQTGYALLWTATAMAL
                     MMFASRRRLRAPWLIGAALLGATVLKLFVIDLANTGGTERIVAFIGVGALMLLVGYVA
                     PLPPAASVAKQPEAT"
BASE COUNT          331 a          664 c          641 g          214 t
ORIGIN      
        1 agaccggcag tggctgcggt gtctcgcgcg gcggttccgc gacggagggc gatgtcaacg
       61 cgaatggcag cgcttcgccc gctgcgttga acacccgcaa gtcggcgagg tctggagtct
      121 ggctatagcg gacgacgtct gcggtcagcg gggcgcggaa gtgcggcccc gcgccctgtg
      181 tggcgagtcc gaagcgcgcg ccgaagtcac ccggcttcag ctcggccgcg tgcgctggca
      241 gcacgagcgc cagcgcggtc aaggcaggca gcatgttcac gtcgcctccg gttgtttggc
      301 cacgctcgcc gccggcggca ggggcgcgac gtaaccgacc agcagcatca gcgcgccgac
      361 gccgatgaac gcgacgatcc gctcggtgcc gccggtgttg gccaggtcga tgacgaagag
      421 cttcagcacc gtggcgccga gcagcgccgc accgattagc catggcgcgc gcaggcgacg
      481 gcgcgatgcg aacatcatca gcgccattgc ggtggcggtc cacagcaggg cgtagccggt
      541 ctgcacgacg aaggactcga aaagccccgc cgcatcccat tcgacgccga agtagtgatg
      601 cgcgatccgc agccacacgg aattgagcca cacgaagaga gccgcgccga tcgtggcgat
      661 ccacaccggt tccgagcgca gcgccgccgg cagtggccag tccgtcgcgg cgattcgcgc
      721 gcgccactgc cacgcggccg cgagtgcgag cagcaccgac agatcggtcg ggttcagcag
      781 cggcacgtgc ggcagcggtg tggcgtcgcc gcgctgcgtg agtgcgctca gcagcgcgcc
      841 cacgaccgcg aatccggcaa ccggcagcag tccgagccag ccgtacgcgc gcgcgtaacg
      901 gttcatcggc cagcgcgctc gcccggccgg cacggtggcc gcctgtgtga tcgcaaacag
      961 caccgcggcg agcgcagcca gcgacgccgc cgggccccag ctcgtgttcc acagttcggc
     1021 gcggtcgatc aggtcgacgc cgaggctgcc cgcgagcagt accagcaggt acacaccgcc
     1081 cgcgtgcacg gcgacccacc agcgcttcaa cgctgcgtcg agcgtgcgca gcgtgaagag
     1141 atgcgcagcg atcgccagcg gccagcagat ccagcccagg tgctcgaagt cgtgccggta
     1201 gatcaatacg ccgaacaccg ccaccagcag catcgccggc agcgacgcgt acgcgggcca
     1261 ggtggcgacg cgccacgggc gattcagccc ggcgtgacac gccacgctcg cgctcagcac
     1321 gtatgccgcc atcagcggga acaggcggtc gagcggctcg accgcgaatt cgagcgtgcc
     1381 gggcccgccg taataccggc gcgtcagttc gagcacgatt gcagccatcc accaggcaaa
     1441 gccgtatagc gcgagcggag caccgagcgc gcgttcgaac tcgtcccagc cgtggccctt
     1501 cacatagcga ttggcgcgct ccgaaatcga ccggccgcag aacgccgccg tgccggccaa
     1561 caggacagcg ccgatgaagt cggcgttcgc aagcggccac ccctgtggcg gacggaagcc
     1621 gaggtgacgc aggaacgcca tcgcagcgcc aagctgcagc agcaggccga acgcgcgcgc
     1681 gagccgcctg ccctgtcgcg cgcccaccca gaacacgcct gcgccttcca gcgcccacgc
     1741 ggcggcggtc cagcgcgcgt cgagcgcgaa cggaatcgcc agggacgcga agatcacgcc
     1801 gagcgcgatg aagcactcga ccagcagccg caatcgctcg agccggcgct
//
LOCUS       DBNHBY010000557         3501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1729157, whole genome shotgun sequence.
ACCESSION   DBNHBY010000557 DBNHBY010000000
VERSION     DBNHBY010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1729157"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..165
                     /locus_tag="AC16HB_13630"
     CDS             7..165
                     /locus_tag="AC16HB_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015302.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HIR5607874.1"
                     /translation="MLAFRFGTGTASLHEVLITLFVFLTAPISAHLLMKAALKIDAGT
                     RVEPPHSQ"
     gene            complement(232..354)
                     /locus_tag="AC16HB_13635"
     CDS             complement(232..354)
                     /locus_tag="AC16HB_13635"
                     /inference="COORDINATES: protein motif:HMM:NF022603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HIR5607875.1"
                     /translation="MLIARIAYFRAEARGFAPGHELSDWYAAEAELDAQLAPDR"
     gene            complement(721..1836)
                     /locus_tag="AC16HB_13640"
     CDS             complement(721..1836)
                     /locus_tag="AC16HB_13640"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIR5607876.1"
                     /translation="MSELDFKSSAPLTMGIELELQILNRHDGDLTLGANDLLRAVKRR
                     KHPGDIKPEITESMIEISTSVHARFATALQELIGIRDALVAAADRLNLLIAGGGAHPF
                     QRWSERQIYDTPRFRQVSQLYGYLAKQFTIFGQHVHIGCADGDAAMRLLHAMSRYIPH
                     FIALAASSPYHQGVDTGFDSSRLNSVFAFPLSGRAPLVDRWEKFVAFFDKMRAAKVVE
                     SMKDFYWDIRPKPEFGTIEIRVFDTPLTVHKAAALAAYAQTLARLLLHEPPVEIGDDL
                     YLAYTYNRFQACRFGLDGLLIDPRTHEHVPIHRDIADTCTRLAPHAQALESTAAIAQI
                     AQWAATRANDARWMRERFAATESLSDLMWKQSQRWRE"
     gene            complement(1833..3056)
                     /locus_tag="AC16HB_13645"
     CDS             complement(1833..3056)
                     /locus_tag="AC16HB_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR5607877.1"
                     /translation="MQLLDLPFLPEWPPAIGPVAWVAVLLFAAILAGEAARRWLATSR
                     VIGYLAVGCLLGPQLAGVIDRATLAQLRVVVDIAFGLLLFELGQRVDLGWLRRNPWLL
                     VTSVFEAALTFVAVFLLLLLFDVRPVAAAAVGVISVATSAAIVITAVKDLRAQGQVTE
                     RILLFTALNTAYAVVGLTLMFGWLHFESASPVLTVLLHPLYLLAGSLAAAGVLAWVLI
                     RLLRVFGRRPSFQFALTLAMVLLTVAIAGLFRLSVPLTLLALGVLARLIDRDRHFVSL
                     RFPETAMLFVVLLFALTGASLEFRGWAAALPLAVGIVAARFVGKLVPVLLLARPSSIT
                     MRKASLINFGLAPMSALALLMLDELTREAPLIAQEIAIGMHLAITILAFLGPSLLYYA
                     LRAAGEAVADEGAGR"
     gene            3302..>3501
                     /locus_tag="AC16HB_13650"
     CDS             3302..>3501
                     /locus_tag="AC16HB_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607878.1"
                     /translation="MNRIRRSFACVAALLAVAAPNVIAQTEGRTALGGTYVSGGIGDG
                     EIARLDARRDKFSLWVITAAKLS"
BASE COUNT          587 a         1187 c         1200 g          527 t
ORIGIN      
        1 gcctcgatgc tcgcgttccg attcggcacc gggacggcga gcctgcacga ggtgctgatc
       61 acgctgttcg tgttcctgac cgcgccgatc tcggcgcacc tgctgatgaa ggcggcactg
      121 aagatcgacg ccggtacgcg ggtcgagccg ccgcactctc aataggatgc gagcgcgcgg
      181 cgattccgac cgtcccgaag tcgctcggac gtcgtttcgt ccatcggaga atcagcggtc
      241 tggcgcgagt tgcgcgtcga gttccgcctc ggccgcgtac cagtcggaca actcgtgccc
      301 gggcgcgaac ccgcgtgcct cggcgcgaaa gtaagcgatc cgggcgatca gcacttcgcg
      361 ttcagcgggt gtcggcgtcg cttgcgcgcc ggacgctctg tcccgcgctg gggttgtcgt
      421 cggctgcgac ttcgacaccg gcttcttccc ggccctgacc ggcgcggcgg acgatgccga
      481 ccgtttgctg tcggccgcgg ctctgcgcga acgtttcttt acggcgcctt cggacggcgc
      541 caacttgcct gcgttcattt cgacttccct tcccgctctg tctcgcagcg ctgcaccggc
      601 gggcagcgat cctgcgccgc ttgcacgacc gatagcagaa ggaagtgaca agaagttgac
      661 gctgcgcagg ttgggtcccg ccagtgggtg acccgaatcc gcgacggcct ggtcactccc
      721 tcattcgcgc cagcgctggc tctgcttcca catcaggtcc gacagcgact cggtggccgc
      781 gaaccgctcg cgcatccagc gcgcgtcgtt ggcgcgcgtt gctgcccact gcgcgatctg
      841 cgcgatcgcg gcggtcgact cgagcgcctg cgcgtgcggc gcgagacgcg tgcaggtgtc
      901 ggctatgtcg cggtggatcg gcacgtgctc gtgcgtgcgc gggtcgatca gcagtccgtc
      961 gagaccaaag cggcacgcct ggaagcggtt gtacgtatag gcgaggtaca agtcgtcgcc
     1021 gatttcgacc ggcggctcgt gcagcagcag gcgcgcgagc gtctgtgcat aagcggccag
     1081 cgccgcggcc ttgtgcacgg tgagcggcgt gtcgaacacg cggatctcga tcgttccaaa
     1141 ctcgggtttc ggccggatgt cccagtagaa gtccttcatg ctctcgacca ctttggcggc
     1201 gcgcatcttg tcgaagaacg ccacgaactt ttcccagcgg tcgaccagcg gcgcacgccc
     1261 cgacagcgga aacgcgaaca cggaattcag ccgcgacgaa tcgaagccgg tatccacgcc
     1321 ctggtggtac ggcgacgagg ccgcgagggc gatgaagtgc gggatgtagc ggctcatcgc
     1381 gtgcagcaga cgcatcgcgg cgtcgccgtc cgcgcagccg atgtgcacgt gctgaccgaa
     1441 gatggtgaac tgcttggcga ggtagccata cagctgcgac acctggcgga agcgcggcgt
     1501 gtcgtagatc tgccgttcgc tccagcgctg gaacggatgc gcgccgccgc cggcgatcag
     1561 caggttcagg cgatcggccg cggcgaccag cgcgtcgcgg atgccgatca gctcctgcag
     1621 cgcggtcgcg aagcgcgcgt gcacgctggt ggagatctcg atcatgcttt cggtgatctc
     1681 gggcttgatg tcgcccggat gcttgcggcg cttgacggcc cgcagcagat cgttggcgcc
     1741 cagcgtcagg tccccgtcat ggcgattgag gatctgcagt tcgagttcga tccccatcgt
     1801 cagcggcgcg gaagacttga agtcgagttc gctcaccgtc ccgctccttc gtccgcgacc
     1861 gcttcgccgg cggcgcgcag cgcgtagtac agcagcgacg ggccgaggaa ggccaggatc
     1921 gtgatcgcca ggtgcatgcc gatcgcgatc tcctgtgcga tcagcggtgc ctcgcgcgtc
     1981 agttcgtcga gcatcagcag cgccagcgcc gacatcggcg ccagcccgaa gttgatcagc
     2041 gacgccttgc gcatcgtgat gctgctcggc cgcgccagca gcagcaccgg caccagtttg
     2101 ccgacgaagc gcgcggcgac gatgccgacc gcgagcggca gcgccgccgc ccagccgcgg
     2161 aactcgaggc tggcgccggt cagcgcgaac agcagcacga cgaacagcat cgcggtctcc
     2221 ggaaagcgca gcgatacgaa gtgccggtcg cggtcgatca gtcgcgccag cacgccgagc
     2281 gcgagcagcg tcagcggcac cgacagcctg aagagcccgg caatcgccac ggtcagcagc
     2341 accatcgcca gcgtcagcgc gaactggaac gacgggcgcc gaccgaacac gcgcagcagc
     2401 ctgatcagca cccatgccag cacgcccgcc gcggcgagcg agccggccag caggtacagc
     2461 gggtgcagca gtaccgtcag cacggggctt gcgctctcga agtgcagcca gccgaacatc
     2521 agcgtgaggc cgaccaccgc gtacgcggtg ttcagcgcag tgaacagcag gatgcgctcg
     2581 gtcacctgtc cctgcgcgcg caggtccttg accgcggtga tcacgatcgc cgccgacgtg
     2641 gccaccgaga tcacgccgac cgccgcggcg gcgaccggcc gcacgtcgaa cagcagcagc
     2701 agcaggaaca cggcgacaaa ggtcagcgcc gcctcgaaca cgctggtgac gagcagccac
     2761 ggattgcggc gcagccagcc gaggtcgacg cgctggccga gctcgaacag cagcagcccg
     2821 aacgcaatgt cgaccacgac gcgcagctgc gcgagcgtgg cgcgatcgat cacaccggcg
     2881 agctggggcc cgagcagaca gcccacggcg agatagccga tcacgcgcga cgtcgcgagc
     2941 cagcggcgcg cggcttcacc ggctaggatg gcggcaaaca gcaacaccgc gacccacgcc
     3001 accggcccga tcgcgggcgg ccactcgggg agaaacggca gatcgagcag ctgcacgcgg
     3061 atccctttgc agtcgagaaa gttgccaaac gatgtccccg aaatgtaggc gattgcggcg
     3121 gattgcgggc cggagtcttc gcgtgccgga ttccgtggac gcgtagggac tacctccgat
     3181 tgacgcacgc acccgctcgc cggcgcagtt gacctgtggc atgtccgcgc tccgggtgcg
     3241 cggcgaccgc gggcggctgg gatcagaatt gcctcgcgct catggcagat aggaggtcga
     3301 gatgaaccgc atccgcagat cgttcgcctg cgtcgccgcg ctgctggcgg tcgcggcgcc
     3361 gaatgtcatc gcgcagaccg agggccgcac cgcgctcggg ggcacgtatg tgtcgggcgg
     3421 catcggcgac ggcgagatcg cccggctcga cgcccggcgc gacaagttca gcctgtgggt
     3481 catcaccgcc gccaagctgt c
//
LOCUS       DBNHBY010000558         4307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1729178, whole genome shotgun sequence.
ACCESSION   DBNHBY010000558 DBNHBY010000000
VERSION     DBNHBY010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1729178"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..2944)
                     /locus_tag="AC16HB_13655"
     CDS             complement(92..2944)
                     /locus_tag="AC16HB_13655"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR5607879.1"
                     /translation="MTATTTNAPLARSFEPAAIERRWYPVWESRGYFAAGLDPNKPAF
                     SIQLPPPNVTGILHMGHAFNQTIMDTLTRYHRMRGFNTLWLPGTDHAGIATQIVVERQ
                     LEQQGVNRRQMKREDFVAKVWEWKQFSGDAILQQMRRLGDSVDWNRTYFTMDPHLSQV
                     VVDTFVRLYDEGLIYRGQRLVSWDPKLQTAVSDLEVTSEEEDGKLWEIRYPALDGGPG
                     VIVATTRPETMLGDVAVAVHPADERYSALVGQRLRLPLAEREIPVIADDYVDREFGTG
                     CVKITPAHDANDYAIGQRHGLKPISIFTLTATISDNAPAQYRGMDRYVARQAVLDDLR
                     AAGLLVSEKPHKMMVPRCERTGEAIEPMLSDQWYVAMSKPAPAGSLHAGKSLAQVALE
                     VVERGEVRIFPDQWKTVYRQWLENIQDWCISRQLWWGHQIPAWYDEAGNVFVARSEAE
                     AQAKAGPGGMLMRDPDVLDTWYSSALVPFSTLGWPAVDKAAAGPERTAYDLYLPSSVL
                     VTGYEIIFFWVARMIVMTTHFTGRVPFRDVYIHGMVRDHEGKKMSKSEGNTIDPVDLL
                     DGIDLQALIEKSTSGLRRPENAPRVAARVRKEFPDGMPAYGADALRFTMASYATLGRN
                     INFDTKRCEGYRNFCNKLWNATAYVLMNAEGRDCGLDESAPVGLSFIDRWIVGELQRL
                     EAEVERGFAEYRLDNVANAIYAFAWNEYCDWYIELTKVQLRAGDAAQQRGTRRTLVRV
                     LETLLRLAHPVIPFITEELWQRISVLAGRRRADYETSVMTQPYPKSEPAQIDAAADRE
                     VAQLKRLIDAVRNLRGEMNLSPAQKVPLAASGPRAQVDAFAPYIAALARLSEVRRVDD
                     LATTAQGSAAPVAVVDEYRLMLAIEVDVAAERERLGKEIARLEGEIRKAEDKLGNASF
                     VARAPATVVAQEKERLANFGATLAKVREQFARLPSA"
     gene            3040..>4307
                     /locus_tag="AC16HB_13660"
     CDS             3040..>4307
                     /locus_tag="AC16HB_13660"
                     /inference="COORDINATES: protein motif:HMM:NF028457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 and DUF5118 domain-containing protein"
                     /protein_id="HIR5607880.1"
                     /translation="MNRFFFQCAVLALATAALGACATVPVAQPPAKPAAAAPASPSAA
                     PASAAAPTTPAARPGETPLRPFADVIKDARETKGFFTLWQKDEKVWIEIRPEQLDQPF
                     LFSTVLARGIAQLPFVPGLLGNSEVASFHRVGNQLQLIARNTMFRTPDNSPLARAARD
                     SVSDSIIASAAVASAPHAERKSFLVEANALLLADVAGIGTLIDAAYRIGYALDARNSS
                     IERARSAAADTTIAVNLHYAVAKLPVPPTTPPPPGTPTPTPPRAVADPRSFLLGIQYR
                     FAQQPDPPMVARIADERVGYFVDEQRDLARDYAQDSRVRVVNRWRLEKQDPNAALSEP
                     KQPIVAYLDRNIPADLRPAVEAGVLEWNKAFEQAGFRNAIVVRQQPDDADWDTLEGRH
                     MAVKWFVDSSLAGTAAIGPHQSDPRTGEIL"
BASE COUNT          639 a         1553 c         1403 g          712 t
ORIGIN      
        1 gatcggtgtg tccggcgcgc cgggtgggga tcaggacgaa ctgtgcgcga aggccggcat
       61 tgcagccatc cgcgacgacg tcgacatgta gctacgcgga aggcagtcgc gcgaactgct
      121 cgcgcacctt ggccagcgtc gcgccgaaat tcgccagccg ctccttctcc tgcgcgacga
      181 ccgtcgccgg agcgcgcgcg acgaagctcg cgttgccgag cttgtcttcg gctttcctga
      241 tctcgccttc cagccttgcg atctccttgc cgagccgctc gcgctcggcg gccacatcga
      301 cctcgatcgc cagcatcagc cggtactcgt ctacgaccgc gaccggcgcc gcgctgccct
      361 gcgctgtcgt cgcgaggtcg tcgacgcgcc ggacctcgga gagccgggcc agcgccgcga
      421 tgtagggcgc gaacgcgtcg acctgcgcgc gcggcccgct cgctgccagc ggcaccttct
      481 gcgccggcga aaggttcatc tcgccgcgca ggttgcgcac cgcgtcgatc aggcgcttca
      541 actgcgccac ctcgcgatcg gcggcggcgt cgatctgcgc cggctcggac ttcggatacg
      601 gctgggtcat caccgacgtt tcatagtcag cgcggcgccg gccggcgagc accgagatgc
      661 gctgccacag ctcctcggtg atgaacggga tgaccggatg cgccagtcgc agcagcgtct
      721 cgagcacgcg caccagcgtg cggcgcgtgc cgcgctgctg tgccgcgtcg ccggcgcgca
      781 actgcacctt tgtcagctcg atgtaccagt cgcagtactc gttccacgcg aacgcataga
      841 tcgcgttggc cacgttgtcg agccggtact cggcgaagcc gcgttcgacc tccgcttcca
      901 gccgctgcaa ctcaccgacg atccagcggt cgatgaacga cagcccgacc ggcgcggact
      961 catccagtcc gcagtccctg ccttccgcgt tcatcagcac gtacgcggtc gcgttccaca
     1021 gcttgttgca gaagttgcgg tagccctcgc agcgcttggt gtcgaagttg atgttgcggc
     1081 cgagcgtggc gtaactcgcc atcgtgaaac gcagcgcgtc ggcaccgtac gcgggcatgc
     1141 cgtccgggaa ctccttgcgc acccgcgccg cgaccctggg cgcgttctcg gggcggcgca
     1201 ggccgctggt cgacttctcg atcagcgcct gcaggtcgat gccgtcgagc agatcgaccg
     1261 ggtcgatcgt gttgccctcc gacttcgaca tcttcttgcc ttcgtggtcg cgcaccatgc
     1321 cgtggatgta cacgtcgcgg aacggcacgc gcccggtgaa gtgggtcgtc atcacgatca
     1381 tccgcgcgac ccagaagaag atgatttcgt agccggtcac cagcaccgac gagggcaggt
     1441 acaggtcgta ggcggtccgc tccggtccgg ccgcggcctt gtcgaccgcg ggccagccga
     1501 gcgtggagaa cggcaccagc gccgacgagt accaggtgtc gagcacatcc gggtcgcgca
     1561 tgagcattcc gccgggtccg gccttggcct gcgcctccgc ctcgctgcgc gccacgaaaa
     1621 cgttgcctgc ttcgtcgtac cacgccggga tctggtggcc ccaccacagc tggcgcgaga
     1681 tgcaccagtc ctggatgttc tccagccact gccggtacac cgtcttccac tggtccggaa
     1741 agatcctgac ctcgccgcgc tcgacgactt cgagcgcgac ctgcgcgagc gacttgcccg
     1801 catgcagcga accggccggc gcgggcttgc tcatcgccac gtaccactgg tccgacagca
     1861 tcggctcgat cgcctcgccg gtacgctcgc agcgcgggac catcatcttg tgcggcttct
     1921 ccgacaccag cagcccggcc gcgcgcaggt cgtcgagcac cgcctggcgc gcgacatagc
     1981 gatccatgcc gcgatactgc gccggcgcgt tgtcgctgat cgtcgcggtc agcgtaaaga
     2041 tcgagatcgg cttcagtccg tgccgctggc cgatcgcgta gtcgttggcg tcgtgcgccg
     2101 gcgtgatctt cacgcagccg gtgccgaact cgcggtcgac gtaatcgtcg gcgatcaccg
     2161 ggatctcgcg ctcggcgagc ggcagccgca gccgctggcc cacgagcgcg ctgtagcgct
     2221 cgtcggccgg gtgcaccgcc accgcaacgt cgcccagcat cgtctcgggc cgagtggtgg
     2281 cgacgatcac gcccggccca ccgtcgagcg ccgggtagcg gatctcccac agcttgccgt
     2341 cttcttcctc gctcgtgacc tcgaggtcgg acaccgcggt ctgcagcttc gggtcccagc
     2401 tgaccagccg ctggccgcgg tagatcagtc cctcgtcata caggcgcacg aatgtgtcga
     2461 ccaccacctg cgacaggtgc gggtccatcg tgaagtaggt gcggttccag tcgaccgagt
     2521 cgcccagccg ccgcatctgc tgcaggatcg cgtcgcccga gaactgcttc cactcccaca
     2581 ccttggcgac gaagtcctcg cgcttcatct ggcgccggtt cacgccctgc tgctcgagct
     2641 ggcgttcgac gacgatctgc gtggcgatgc ctgcgtggtc ggtgccgggc agccacagcg
     2701 tgttgaagcc gcgcatccgg tgatagcgcg tcagcgtgtc catgatcgtc tggttgaacg
     2761 catggcccat gtgcaggatg ccggtcacgt tcggcggcgg cagctggatg ctgaaggcgg
     2821 gcttgttcgg gtccagcccg gcggcgaaat agccgcgcga ttcccacacc gggtaccagc
     2881 gccgctcgat cgcggccggt tcgaagcttc tggccagcgg tgcgttcgta gtcgtcgccg
     2941 tcatttcgtc gcgctttccg tcggttcatc gatgtcgaat tgaattttag gggaatcgtc
     3001 tctaggctcc gcgctctgtt cacagccgtc ctcaccacca tgaaccgctt cttcttccag
     3061 tgcgccgtgc tcgcgcttgc gacggcggcg ctcggcgcct gcgccaccgt cccggtagcg
     3121 cagccgcccg ccaagcccgc ggccgctgcg cccgcgagtc cgagcgccgc gccagccagc
     3181 gcggcggcac ccacgacgcc tgcggcgcgc cccggcgaaa cgccgctgcg cccgttcgcc
     3241 gacgtgatca aggatgcccg ggagaccaag ggcttcttca cgctgtggca gaaggatgaa
     3301 aaggtctgga tcgagatccg gcccgagcag ctcgaccagc cgttcctgtt cagcacggtg
     3361 ctcgcgcgcg gcatcgcaca gctgccgttc gtgccgggct tgctcggcaa ttccgaggtg
     3421 gcgagctttc accgtgtcgg caaccagctg cagctcatcg cgcgcaatac catgtttcgc
     3481 acgcccgaca actcgccgct ggcgcgcgcg gcgcgcgaca gcgtgtccga cagcatcatc
     3541 gcgtcggcgg cggtcgcctc ggcgccgcac gcggagcgca agtcgttcct ggtcgaggcc
     3601 aacgcgctgc tgctcgccga cgtcgccggc atcggcacgc tgatcgacgc cgcgtaccgg
     3661 atcggctatg cgctcgatgc gcgcaacagc tcgatcgaac gcgcgcgcag cgccgccgcg
     3721 gacaccacaa tcgcggtgaa cctgcactac gcggtggcca agctgccggt gccgccgacc
     3781 accccgccgc cgccgggcac gcccacgccc acgccgccgc gcgcggttgc ggacccgcgc
     3841 agcttcctgc tcggcatcca gtaccgcttc gcgcagcagc ccgacccgcc gatggtggca
     3901 cgcattgccg acgaacgcgt cggctatttc gtcgacgagc agcgcgacct cgcgcgcgac
     3961 tacgcgcagg attcgcgcgt gcgcgtggtg aaccgctggc gcctggagaa gcaggatccg
     4021 aacgccgcgc tgtcggagcc gaagcagccg atcgtcgcct atctggaccg caacattccg
     4081 gccgacctgc ggcccgcggt cgaggccggc gtgcttgagt ggaacaaggc attcgagcag
     4141 gcgggcttca ggaatgcgat cgtcgtcagg caacagcccg acgacgccga ttgggacacg
     4201 ctcgagggcc ggcacatggc ggtcaagtgg ttcgtcgact cgagcctcgc cggcacggcc
     4261 gcgatcggcc cgcaccagag cgacccccgg accggcgaaa tcctgta
//
LOCUS       DBNHBY010000559         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_713962, whole genome shotgun sequence.
ACCESSION   DBNHBY010000559 DBNHBY010000000
VERSION     DBNHBY010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_713962"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16HB_13665"
     CDS             <1..264
                     /locus_tag="AC16HB_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607881.1"
                     /translation="LPSVLQRAPAEGRQSYAVTVAFTNSGAAFTLTATPCATASCTGS
                     NVDSFSDAVCGNYQLDNTDARAVVIGGTTYSGAATQVASCWQR"
     gene            306..731
                     /locus_tag="AC16HB_13670"
     CDS             306..731
                     /locus_tag="AC16HB_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR5607882.1"
                     /translation="MAASSRHTPTDITLHTASRMLEIAFDDGRVFKLPFEFLRVLSPS
                     AEVRGHGPGQETLQFGKRDVTITALEPVGHYAVQPTFSDGHASGIFSWDYLYELGANQ
                     ERLWADYLARLEAAGASRDPDDPRNAAFVMSGGPSCGHH"
     gene            736..1485
                     /gene="ubiE"
                     /locus_tag="AC16HB_13675"
     CDS             736..1485
                     /gene="ubiE"
                     /locus_tag="AC16HB_13675"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR5607883.1"
                     /translation="MAEKSGDPTTHFGFQQVAEEEKAQRVAQVFDSVASKYDLMNDLM
                     SAGLHRLWKAFTVRQADVRPGMKVLDIAGGTGDLARAFAKRAGPQGVVVLTDINGAML
                     REGRDRLLDRGLMLPLVQCDAERLPFASGTFDVVSVAFGLRNMTHQDMALAEMRRVAR
                     PGGKVMVLEFSRIWQPLAPVYDAYSFNVLPWLGKKIAGDSDSYRYLAESIRVHPSQEE
                     LARLMERAGLESVQWFNLTAGVCALHVGRKI"
     gene            1523..>2429
                     /locus_tag="AC16HB_13680"
     CDS             1523..>2429
                     /locus_tag="AC16HB_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR5607884.1"
                     /translation="MVGLLAVSVSLDADAQRRLGGGKTLGKPSQTLQQRQATPPPQQT
                     SPAQQAAPTQQPAPAAATGAAAAPKPASPLRGALMGLAAGLGLAALASWLGFGETLAT
                     IMLVVLAGLLAMMVVGYLMRRSGGQPAYQGAGNGRYPPQPPEAMPRPIEVTPRSAERT
                     SFEPAAARPGSAMDEFKHGTTAKLDQPWNIPAGFDTESFLANAKSYFTRLQTAWDKSN
                     LDELSEFTTNEMFTALTHELRARAGASRSEVVTLDARLLGIESDANEHLASVRFTGTL
                     RVDGELEQFDEVWNLTKSTDGKTGWL"
BASE COUNT          414 a          792 c          837 g          386 t
ORIGIN      
        1 ctgccgtcgg tcctgcagcg cgcccccgcg gaggggcggc agagttatgc ggtgaccgtg
       61 gcattcacca acagcggggc ggcatttacc ttgacggcga ccccgtgcgc caccgccagc
      121 tgtaccggtt cgaacgtcga cagcttcagc gacgcggtgt gcggcaacta tcagctcgat
      181 aacaccgatg cgcgcgcggt cgtgatcggc ggcacaacat acagcggcgc cgcgacgcag
      241 gtggcgtcct gctggcaacg ctgactctcc gcacgcgctg attcgagtcc ggctatgctt
      301 gacgcatggc cgcctcgagt cgacacaccc caaccgacat caccctgcat accgcctcgc
      361 ggatgcttga gatcgcgttc gacgacgggc gcgtgttcaa gctgccgttc gagttcctgc
      421 gtgtgctgtc gccgtcggcg gaggtgcgtg gccacgggcc gggacaggag acgctgcagt
      481 tcggcaagcg cgacgtgacg atcacggcgc tggagccggt cggccactac gcggtgcagc
      541 cgacgttctc cgacggccat gcgagcggca tcttcagctg ggactacctg tacgagttgg
      601 gcgcgaacca ggagcgcctc tgggccgact atctcgcgcg gctcgaggcc gccggcgcga
      661 gccgcgatcc ggacgatccg cgcaacgccg cgttcgtgat gagcggcggg ccaagctgcg
      721 gccatcactg acgcgatggc cgagaagtcc ggcgatccga cgacacattt cggcttccag
      781 caggtcgccg aagaagaaaa ggcgcagcgc gtcgcgcagg tgttcgactc ggttgcgtcg
      841 aagtacgacc tgatgaacga cctgatgtcg gccgggttgc accggctgtg gaaggcgttc
      901 accgtgcgcc aggccgacgt gcggccgggc atgaaggtgc tcgatatcgc gggtggcacc
      961 ggcgacctgg cgcgcgcatt cgcaaagcgc gcgggcccgc agggcgtcgt cgttctgacc
     1021 gacatcaacg gcgcgatgct gcgcgaaggc cgcgatcgcc tgctcgaccg cggcctgatg
     1081 ctgccgctcg tgcagtgcga cgccgagcgc ttgccgtttg ccagcggaac attcgacgtc
     1141 gtcagcgtcg cctttggctt gcgcaacatg acgcatcagg acatggcgct ggcggagatg
     1201 cggcgtgtcg cccgccccgg cggcaaggtc atggtgttgg agttttcccg catatggcag
     1261 ccgttggcgc ctgtctacga tgcctattcg ttcaacgtgc tgccgtggct gggcaagaag
     1321 atcgcgggcg acagcgacag ttaccgctac cttgccgaat cgatccgcgt gcatccgtcg
     1381 caggaagaac tcgcgcggct gatggaacgg gccggcctcg aaagcgtcca atggttcaac
     1441 ctgactgcgg gcgtgtgtgc gttgcacgtg ggacgaaaga tctgaaccgg agacgcgaac
     1501 gaagaaccgg ttgatcggcg cgatggtggg cctcttggcg gtttcggtat cgctcgatgc
     1561 cgatgcgcag cgacggctcg gcggcggcaa gacactcggc aaaccttctc agacgttgca
     1621 gcagcggcaa gcgaccccgc cgccacagca gacgtccccc gcgcagcagg ctgctccgac
     1681 gcagcagcca gcgccggcgg cggcaaccgg tgcagcggct gcgcccaaac ctgcgagtcc
     1741 gctcagaggc gcgctgatgg ggctcgccgc gggcctcggc ctcgcggcac tggcatcgtg
     1801 gcttggcttc ggcgagacgc tggcgacgat catgctggtc gtgctggccg gcctgctggc
     1861 gatgatggtc gtgggttatc tgatgcgccg ctccggtggg cagcccgcgt accagggcgc
     1921 cggcaacggg cgctatccgc cccagccgcc ggaggcaatg ccgcggccca tcgaggtgac
     1981 gccgagatcc gcggagcgca cgtccttcga acctgcggcc gcgcgacccg gctcggcgat
     2041 ggatgaattc aagcacggca ccacggcgaa gctcgaccag ccgtggaaca ttccggccgg
     2101 tttcgacacc gaatcgttcc tcgccaacgc aaagagctac ttcactcggc tgcagacggc
     2161 gtgggacaag tcgaacctcg acgaattgtc ggagttcacc acgaacgaga tgttcaccgc
     2221 gctgacgcac gaactgcgcg cgcgcgcagg cgcgagcagg tccgaagtgg tgacgctcga
     2281 cgccaggctg ctcggtatcg aaagcgatgc gaacgagcat ctggcgagcg tgcgcttcac
     2341 cggtacgctg cgcgtcgatg gtgaactcga gcagttcgac gaggtctgga acctcaccaa
     2401 gtccaccgac ggcaagaccg gctggctgc
//
LOCUS       DBNHBY010000560         2885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_104605, whole genome shotgun sequence.
ACCESSION   DBNHBY010000560 DBNHBY010000000
VERSION     DBNHBY010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_104605"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..467)
                     /locus_tag="AC16HB_13685"
     CDS             complement(102..467)
                     /locus_tag="AC16HB_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607885.1"
                     /translation="MPIKKVLIVDDSPTERYFLTELLTKNGFNVSVAENAEEALLKVK
                     SQTPDLILMDIVMPGQNGFQLTRQLSKDPATQGIPVIMCTSKNQQTDRIWGLRQGARD
                     YITKPVKADELLGKIAALS"
     gene            complement(498..893)
                     /gene="pilG"
                     /locus_tag="AC16HB_13690"
     CDS             complement(498..893)
                     /gene="pilG"
                     /locus_tag="AC16HB_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HIR5607886.1"
                     /translation="MATQDFSDIRGRKVMVIDDSNTIRRSAEIFLTQAGCQVILAQDG
                     FEALSKITDHQPSIIFCDILMPRLDGYQTCALIKRSPRFSKTPVVMLSSKDGLFDRAR
                     GRMVGSSEYLTKPFTKDSLLTAVRQHAAE"
     gene            complement(932..1123)
                     /locus_tag="AC16HB_13695"
     CDS             complement(932..1123)
                     /locus_tag="AC16HB_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015389172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR5607887.1"
                     /translation="MTTTTAGNGTRTWMCLICGWIYDEAAGLPDEGIAPGTRWEDLPP
                     NWTCPECGARKEDFEMVEI"
     gene            1285..2118
                     /gene="thiD"
                     /locus_tag="AC16HB_13700"
     CDS             1285..2118
                     /gene="thiD"
                     /locus_tag="AC16HB_13700"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042724 - thiamine-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR5607888.1"
                     /translation="MLAFGASDPTGATGLAADAITCASMGCHATAVVTAVSVQDSVRV
                     QDVQAIDDELVDDQARAVLEDMPVAAIKVGAVGSSDNVQSIAAILSDYDDVPVVVDPS
                     FIAPSADASESRSALELMTAMCELLLPQTTVLTLDLETARRLTAVLAPAGRTDDDDVG
                     AAECARRLLGAGAQYVLLTGVQTAGGLNVNTLFGPDGGQQNESVDRIDRRFRGAGDTL
                     SASIAALLAQGIEVGDAVREAHDFLGQSLAAGFRLGMGAAMPDRLFWAGDEAPQDDDD
                     A"
     gene            2102..2767
                     /gene="thiE"
                     /locus_tag="AC16HB_13705"
     CDS             2102..2767
                     /gene="thiE"
                     /locus_tag="AC16HB_13705"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5607889.1"
                     /translation="MTTTLDRPAGVYLLTPDWTDTARLLETTAAALRNGVCWLQYRNK
                     RADAAVRRTQARALRELTLTCGARLVVNDDADLAIAVQADGVHVGRDDADPRPKLARE
                     GIRMLVGVSCYDDFERARRAQREGADYVAFGSIFASTTKPAAVRAPLTLLARARAGHM
                     HAVAIGGIDATNIERVAAAGAHAAALISAIYDAADPAAAARDLVARFEEGKMQYESQR
                     AAV"
     gene            2742..>2885
                     /locus_tag="AC16HB_13710"
     CDS             2742..>2885
                     /locus_tag="AC16HB_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5607890.1"
                     /translation="MNRNELLFERAQRSIPGGVNSPVRAFRSVGGTPRFVARAEGPYF
                     WDAD"
BASE COUNT          445 a          940 c          989 g          511 t
ORIGIN      
        1 gcgagctgcg tcgagaaatc gcgcagcttg tccagttgct tgtcgggctg catcgacgct
       61 cctccgccgg cctcgttccg tgcgacgccc cggtccatcg gttaactcaa cgccgcgatc
      121 ttgccgagca attcgtcggc cttgaccggc ttggtgatgt agtcgcgcgc accctgccgc
      181 aacccccaga tgcgatcggt ctgctggttc ttgctcgtgc acatgatgac cggaatgccc
      241 tgcgtcgccg ggtccttgga gagctggcgc gtcagctgga atccgttctg tcccggcatc
      301 acgatgtcca tcagaatcag gtcgggtgtc tgcgatttca ccttcagcag cgcttcctcc
      361 gcgttctctg ccacggagac gttgaagccg ttcttggtca gcagttcggt caggaagtag
      421 cgctcagtcg gcgaatcgtc gacgatcagc actttcttga tcggcatgtg cgccctcgcg
      481 ctctcgatgt gggttcgtca ctccgcggcg tgctggcgca cggcggtgag caggctgtcc
      541 ttggtgaacg gcttggtcag atactcgctg gagccgacca tgcggccgcg cgcacggtcg
      601 aacaagccgt ccttcgacga cagcatcacg accggagtct tggagaagcg cggactgcgc
      661 ttgatcagcg cgcaggtctg gtagccgtcc agccggggca tcaggatgtc gcagaagatg
      721 atgcttggct gatggtcggt gatcttggac agtgcctcga agccgtcctg cgcgaggatg
      781 acctgacagc cggcctgggt caggaagatt tcggcgctgc ggcgaatggt gttcgaatcg
      841 tcgatcacca tcaccttgcg gccgcggatg tcggagaagt cctgtgtggc catcgcatga
      901 ctccggattc agcgtgcacg cggccgccgc gtcagatctc caccatctcg aagtcctcct
      961 tgcgcgcgcc gcactccggg caggtccagt tgggcggcag gtcttcccag cgggtgccgg
     1021 gcgcgattcc ctcgtccggc agccccgccg cctcgtcgta gatccaaccg cagatcaagc
     1081 acatccatgt acgcgtccca ttgcccgctg tggttgttgt catggcggta aagcattgcg
     1141 gaagagtcgg ctaacatccg tcgaatcgat cataacttgg gctgcgcagt tacggcttac
     1201 tttcggcttc atacatacgg ctgaggggtt gccagtgcgg catccaactc tcgcacgctg
     1261 atggctgttc atgcaccgcc cgtggtgctg gccttcggag cgtcggatcc caccggcgcg
     1321 accgggctgg ccgccgatgc gatcacgtgc gcttcgatgg gctgtcacgc gaccgcggtg
     1381 gtcactgccg tgtcggtgca ggacagcgtg cgtgtgcagg acgtgcaggc gatcgacgac
     1441 gaactggtgg acgaccaggc gcgcgcggtc ctcgaggaca tgccggtggc cgcgatcaag
     1501 gtcggcgcgg tcggcagcag cgacaatgtg cagtcaatcg cagcgatcct gtccgactac
     1561 gacgacgtgc cggtcgtggt cgacccgtcg ttcatcgcgc cgagcgccga cgcgagcgaa
     1621 agccgatcgg cgctggaact gatgacggcg atgtgcgaac tgctgctgcc tcagaccacc
     1681 gtgttgaccc tcgacctcga aaccgcgcgg cgcctcacgg ctgttctcgc acccgccggc
     1741 cgcaccgatg acgacgacgt gggcgcggcg gaatgcgcgc gccggctgct cggcgccggc
     1801 gcgcagtacg tgctgctgac cggcgtgcag accgcgggcg gcctgaacgt caacacgctg
     1861 ttcggccccg acggcgggca gcagaacgaa agcgtggatc gaatcgatcg gcgcttccgc
     1921 ggtgccgggg acacactgtc ggcttcgatc gccgcgctgc tcgcgcaggg catcgaggtc
     1981 ggcgacgcgg tgcgcgaagc gcacgacttc ctcggccagt cgctggccgc gggttttcgg
     2041 ctcggcatgg gggcggcgat gccggaccgc ctcttctggg ccggcgacga ggcgccgcag
     2101 gatgacgacg acgcttgacc ggcccgccgg tgtctatctg ctgacgcccg actggaccga
     2161 taccgcgcgc ctgctggaga caacggccgc agcgttgcgg aacggcgtgt gctggctgca
     2221 gtaccgaaac aagcgcgccg acgccgcggt gcggcggacc caggcgcggg cactgcgcga
     2281 actgaccctg acctgcggcg cacggttggt ggtcaacgac gacgcggacc tcgcgatcgc
     2341 cgtgcaagcc gacggcgttc atgtgggccg cgacgatgcc gatccgcgac cgaagctcgc
     2401 gcgcgagggg atccggatgc tggttggcgt gtcgtgctac gacgacttcg agcgtgctcg
     2461 gcgcgcgcag cgcgaaggcg cggactatgt ggcgttcggc tcgatcttcg cctccacgac
     2521 caagccggcc gcagtgcgcg ctccactgac gctgttggcg cgggcgcgcg ccggacacat
     2581 gcacgcagtc gcgatcggtg gtatcgatgc gaccaacatc gaacgcgtcg cggcggcggg
     2641 cgcacatgcg gccgcgctga tcagcgcgat ctacgatgcg gccgaccccg cggcagccgc
     2701 gcgcgatctc gtggcccgtt tcgaagaggg gaagatgcaa tatgaatcgc aacgagctgc
     2761 tgtttgagcg cgcgcagcgg agcattcccg gcggcgtcaa ctcgccggtg cgcgcattcc
     2821 gttcggtcgg aggcaccccg cgcttcgtcg cgcgcgcgga ggggccgtac ttctgggacg
     2881 ccgac
//
LOCUS       DBNHBY010000561         6105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1662370, whole genome shotgun sequence.
ACCESSION   DBNHBY010000561 DBNHBY010000000
VERSION     DBNHBY010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1662370"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="AC16HB_13715"
     CDS             complement(<1..350)
                     /locus_tag="AC16HB_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PmbA/TldA family metallopeptidase"
                     /protein_id="HIR5607891.1"
                     /translation="MPFDIETLAQRFRRLVPAANHCALRVVEETSETISVRQDVPEPT
                     SKFVDRGAMVTLIDGDGIGYAATRDLSDAALSAAIARAAEWARLSRGRSVFAGEPPRL
                     PAARGLYASRTTQPV"
     gene            519..1415
                     /locus_tag="AC16HB_13720"
     CDS             519..1415
                     /locus_tag="AC16HB_13720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003272573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5607892.1"
                     /translation="MSEQDSELLQAAQRHIDSHQPPLALDRRGFVASTLSTGFALAVL
                     PVQAQTVIKTDASGLAAGPVRIPVKDGEIPAYRAMPTGRTGLSTILVAHEAWGVHEYI
                     QDVCRRLARLGYMAVAPELFARAGDVGKAESIGVIVRDFMGKTPTAQFASDLDATAAW
                     AAKNGGDADRLGITGFCWGGRAAWMYAAGNPRLKAAVAWYGALSSAFAPGDKSVLDSA
                     AQIRVPVLGLYGARDNGIPVADVERMRAALKAAGNDKCEFHVYPNAGHGFHADYRSTY
                     VKDAAEDGWKRMADWFRSHGLT"
     gene            1443..2183
                     /locus_tag="AC16HB_13725"
     CDS             1443..2183
                     /locus_tag="AC16HB_13725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5607893.1"
                     /translation="MITTDSVGVDATSIELPTYDRTIPAYRAQPAGGRGRATVLVVQE
                     IFGVHEHIRDVCRRLAKAGYLAIAPELYARQGDPSRYTDIPTLIGEIVGRVPDAQVMR
                     DLDAAANWAAQHGGSGKLGITGFCWGGRTTLMYAAHNPDVAAAVAWDGPTARAYHAGD
                     RTAVDVAARIKAAVLGLYGGDDAGIPVDTVEQMRDALMSGGNTKSEFVIYPGTPHAFF
                     ADYRPNYRQEAAADGWRRLLAWFGKHLV"
     gene            2248..3027
                     /locus_tag="AC16HB_13730"
     CDS             2248..3027
                     /locus_tag="AC16HB_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5607894.1"
                     /translation="MSTLAWIVLANVVSTLASISLAAALSFRLLSGLVDKLVCVSAGL
                     LLTVATTHLLPESFHSDADPFVLGWVLLAGIMGFFLLEKVALIHHTHHHEGDAHHHDH
                     GHDAQEARRGGWPILIGDAFHNFGDGVVIAAAFLVDVHLGVTATLAVMAHEIPHEVGD
                     FMILLNAGFAKRKAFVFNLISGMSAVAGGVIGYFVLETMQGLLPFALMLAAASFVYIA
                     LSDLLPEMMRRSSLRRSLPEVLLVLAGIAIGALVTGGVHAH"
     gene            complement(3029..3886)
                     /locus_tag="AC16HB_13735"
     CDS             complement(3029..3886)
                     /locus_tag="AC16HB_13735"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR5607895.1"
                     /translation="MHTTLVSVPELRQHLTDPDWCVVDCRHDLTDHSLGLKAYQQGHV
                     PGAVFARIEDDLSGPKTGGNGRHPLPDRAALAQRLRDWGIGNDTQIVAYDANGGSFAG
                     RLWWLARWLGHAKVALLDGGWPAWLAETGLSSVEPVRRASGSFETRDPLVRLIDADAV
                     QAMRSDPNLLLVDVRAPERYRGETEPLDPVAGHIPGAVNRFWQANLTSAAAGKFKSPR
                     ELRAELDALRDGRAPTQIAAYCGSGVTACHLVLALEIAGLPGAAIYPGSWSEWVADRA
                     RPVATGATP"
     gene            complement(3894..4793)
                     /locus_tag="AC16HB_13740"
     CDS             complement(3894..4793)
                     /locus_tag="AC16HB_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5607896.1"
                     /translation="MQSLWMLVAALLFALMGVCVKFATRDYGVMELVFYRGLIGCLGI
                     ALFTRMRNETLATPVPWLHLRRGAVGTMSLALWLYATSLLPLGTAMTLNYSSPLFLAA
                     FSVAAALMAGRRAHWEMAAAVSVGFAGVILVLQPSFDGSQSNAALAGLASGVMAALAY
                     WHIKELGRLGEPEWRIVFYFAATGVAFGLIGALAAGFSPLTAHGLVLLLAVGLTATLA
                     QLAMTRAYGHGRTLLTANLQFSAIVFASILGMAVFDDQLGLASWMGIGLIIASGVIAT
                     ILSARRARPEQPFEETIEPDVDK"
     gene            complement(4874..5842)
                     /locus_tag="AC16HB_13745"
     CDS             complement(4874..5842)
                     /locus_tag="AC16HB_13745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5607897.1"
                     /translation="MQRRLLALSACCVLLSGAPPVLGQPFPARPIRLIVSYPPGGQSD
                     LVGRLVAQQLGEQLDTSIVVENRPGANGTVGIEAGARAASDGYTLVLAGGGGLTLAPL
                     VDSSVRYDALRDFIPLARVSRTPHVLATRAGLGIDTVPQLIDYARRNPGKLTCASAGP
                     GATMAIEAFKTAAGVNILSVPYKGFTPAVLDVASGQVDLVVADVAVIAPFVQSGRIRV
                     IANAGEVRSRAYPDVRTFAEEGLADVVSDSWQSIVVPRGTPADIVARLQNGLQAMVAS
                     TGFRDGLMRLGFETIEEHPDRFPAVLLEELARNRVLARRAGVQVLR"
BASE COUNT         1010 a         2035 c         2098 g          962 t
ORIGIN      
        1 acaggctgcg tcgtccgtga cgcatagagg ccgcgcgcgg ccggcagccg gggcggctcg
       61 cctgcgaaca cgctgcggcc ccgcgatagc cgcgcccact cggcggcgcg cgcgatcgcc
      121 gcgctcagag cggcgtccga cagatctctg gtcgccgcgt agccgatccc gtcgccatcg
      181 atcagcgtga ccatcgcgcc gcggtcgacg aatttcgacg tgggttccgg tacgtcctgt
      241 cgcaccgaga tcgtttcgga ggtttcctcg accacgcgca gcgcgcaatg gttggccgcc
      301 ggcacgagac ggcggaagcg ttgcgccagc gtttcgatgt cgaatggcat agattccttc
      361 gcgtccgccg gattcgcggc ggacaaccgg ggcgccggca cccctcgtgg gggtgcagcg
      421 catgaatttg ggatgcaggg attctaccgg cggcggcaga tgtcaattcc aatctctaca
      481 ttcgcgcgcg ggccttcggc cgcgcattcg ggtggcacat gagcgaacag gacagcgaac
      541 tgctccaggc ggcacaacga cacatcgaca gccatcagcc gccgttggcg ctggaccggc
      601 gcggattcgt cgcgtcgact ttgtcgaccg gctttgcgct ggcggtcttg cccgtgcagg
      661 cgcagacggt gatcaagacc gatgcgagcg gacttgccgc cgggccggtc aggattccgg
      721 tcaaggatgg cgagatcccg gcgtatcgcg cgatgccgac tgggcgcacg ggcctttcga
      781 cgatcctcgt ggcgcacgag gcgtggggtg tgcacgagta catccaggac gtgtgccggc
      841 gcctcgccag gctcggctac atggcggtcg cgccggagct tttcgcgcgc gccggcgacg
      901 tcggcaaggc cgagagcatc ggcgtgatcg tgcgcgactt catgggcaag acgccgaccg
      961 cgcagttcgc gagtgatctc gatgccaccg cggcgtgggc ggcgaagaac ggcggcgatg
     1021 cggatcgtct cggcatcacg ggattctgct ggggcggacg ggctgcgtgg atgtatgcgg
     1081 cgggcaatcc acgtctgaag gccgcggtgg cgtggtacgg cgcgctgtcg tcggcattcg
     1141 ctccgggcga caagtcggtg ctggactcgg ccgcgcagat ccgcgtcccc gtgctggggc
     1201 tgtacggcgc ccgcgacaac ggtattccgg tcgccgacgt cgagcggatg cgcgcagcgc
     1261 tgaaagccgc cggcaacgac aagtgcgagt tccacgtcta cccgaacgcc gggcacggat
     1321 tccacgccga ttaccggtcg acgtatgtga aggacgccgc cgaggacggc tggaagcgga
     1381 tggcggattg gttcaggtcg cacggcctga cctgagcgat cagtctgggg aacggctgca
     1441 cgatgatcac caccgactcc gtcggggtcg atgccacgag catcgaactg ccgacctacg
     1501 atcgaacgat tccggcctat cgcgcgcagc cggcgggcgg gcgcgggcgc gcaaccgttc
     1561 tggtcgtgca ggagatcttc ggcgtgcacg agcacatccg cgacgtgtgc cggcggcttg
     1621 cgaaggccgg ctatctcgcg atcgcgcccg aactgtatgc acggcagggc gacccgtcgc
     1681 ggtacaccga cattccgacg ctgatcggcg agatcgtggg ccgggtgccc gacgcacagg
     1741 tgatgcgcga tctcgacgcg gcagcgaact gggctgcgca gcacggcggg tcaggcaagc
     1801 tcggcatcac gggattctgc tggggcggcc gtacgacgct gatgtacgcc gcacacaatc
     1861 ccgacgtggc cgccgccgtc gcgtgggacg gaccgaccgc gcgcgcttat cacgccggcg
     1921 atcgcacggc ggtcgatgtc gcggcaagaa tcaaggctgc ggtgctcggc ctgtacggcg
     1981 gcgatgatgc cggcattccg gtggataccg tcgaacagat gcgcgacgcg ctgatgtccg
     2041 gcggcaatac gaagtcggag ttcgttatct atcccggcac gccgcatgcg ttctttgccg
     2101 actaccggcc aaattatcgc caggaggccg ccgcggacgg ctggaggcga ctgctggcat
     2161 ggttcggcaa acacctcgtc tgagagtcgc tcgcgtggcc acgcatgccg gcccgcgcgg
     2221 gcttgacatg gctgctaccg tgctcgcatg tccacgcttg cctggatcgt gctcgccaac
     2281 gtggtgtcga cgctcgcgtc gatcagcctc gccgcggcac tcagcttccg cctgctgtcg
     2341 gggctcgtcg acaagctcgt ctgcgtgtcg gccgggttgc tgctgaccgt cgccaccacg
     2401 cacctccttc ccgagtcgtt ccacagcgac gccgatccgt tcgtacttgg ctgggtgctg
     2461 ctggcaggga tcatgggctt cttcctgctg gagaaggtgg cgctgatcca ccatacgcat
     2521 catcacgagg gcgacgcgca ccaccacgat cacgggcacg acgcgcagga agcgcgccgc
     2581 ggcggttggc cgattctgat cggcgatgcg ttccacaact tcggcgacgg cgtggtgatc
     2641 gccgccgcgt tcctggtcga cgtgcacctc ggcgtgacgg cgacgctggc agtgatggcg
     2701 catgagatcc cgcacgaggt cggcgatttc atgatcctgc tgaacgccgg cttcgcgaag
     2761 cgcaaggcgt tcgtgttcaa tctgatctcg gggatgtccg ccgtcgcagg cggcgtgatc
     2821 ggctacttcg tgctcgaaac gatgcagggg ttgctgccgt tcgcgctgat gctcgcggct
     2881 gcgagcttcg tctacatcgc gctgtcggat cttctgccgg agatgatgcg gcgctcgtcg
     2941 ttgcgccgct cgctgcccga agtgctgctg gtgctggccg gcatcgcgat cggcgcgctg
     3001 gtgaccggcg gcgtgcacgc acactgagct acggcgttgc tccggtggcg accgggcgcg
     3061 cccggtcggc gacccactcg ctccacgacc cggggtagat ggctgcgccc ggcaggcccg
     3121 cgatttccag cgcgaggacc aggtgacacg cggtgacgcc ggagccgcaa tacgctgcga
     3181 tctgagtcgg cgcgcggccg tcgcgcagcg catccagttc ggcacgcagc tcgcgcggcg
     3241 atttgaactt gcccgctgca gccgacgtga gattcgcctg ccagaagcgg ttcacggcgc
     3301 cgggaatgtg gccggcaacc ggatcgagcg gctcggtctc gccgcgatag cgctccggcg
     3361 cgcgcacgtc gacgagcagc aggttcgggt cgctgcgcat cgcctgcact gcatcggcgt
     3421 cgatcaacct caccagtgga tcgcgcgtct cgaagctgcc ggacgcgcgc ctcaccggct
     3481 caacgctcga cagtccggtt tccgcgagcc atgccggcca gccgccgtcg agcagcgcga
     3541 ccttcgcgtg tccgagccag cgcgcgagcc accacaacct gccggcgaac gagccgccgt
     3601 tcgcgtcgta agcgacgatc tgtgtgtcgt tgccgatgcc ccagtcgcgc aacctctgcg
     3661 caagtgcagc gcgatcgggc aggggatggc gcccgttacc gccggtcttc gggcccgaca
     3721 gatcgtcctc gatgcgcgca aacaccgcac ccggaacgtg cccttgctga tacgccttga
     3781 gcccgagcga gtgatcggtc agatcgtggc ggcaatcaac cacgcaccaa tcgggatcgg
     3841 tcaggtgctg tcgcagttcc ggcactgaaa cgagcgtcgt atgcatcgtg gtctcacttg
     3901 tcgacatccg gctcgatggt ttcctcgaac ggctgctccg gccgcgcgcg ccgcgccgag
     3961 aggatagtcg cgatcacgcc gctggcgatg atcaacccga tgcccatcca actcgccagc
     4021 ccgagttgat cgtcgaacac ggccataccc agtatcgaag caaagacgat cgcagaaaac
     4081 tgcaggttcg cggtcagcag cgtgcggccg tggccatacg cccgcgtcat cgcaagctgc
     4141 gcgagcgtgg cggtgagccc gaccgccagc agcaatacca gcccgtgcgc cgtgagaggc
     4201 gagaagccgg ccgcgagcgc gccgatcagg ccgaatgcca cgccagtggc cgcgaaatag
     4261 aacacgatcc gccattccgg ctcgcccagg cgtccaagct ccttgatgtg ccagtacgcc
     4321 agcgccgcca tcacgccgga ggccaatccc gcgagcgccg cgttggattg cgagccgtcg
     4381 aagctcggct gcagcacgag aatcacgccg gcgaagccga cgctgactgc agccgccatc
     4441 tcccagtggg cgcgacggcc cgccatcagc gcggcagcca ccgagaacgc ggcgaggaac
     4501 aacggcgacg agtagttcag cgtcatcgcg gtgccgaggg ggagcagcga agtggcgtac
     4561 agccacagcg cgagcgacat cgtgccgacc gcgccgcgtc gcaggtgcag ccaaggcacc
     4621 ggggtggcca gcgtctcgtt gcgcatgcgc gtgaagagcg cgattccgag gcagccgatc
     4681 agcccgcgat agaacaccag ttccatcacg ccgtaatcgc gcgtggcgaa tttcacgcac
     4741 acccccatga gagcgaacag cagcgcggcg accagcatcc agagcgattg catcgggcgg
     4801 ggagtgtagc gatcgcggtg gtgaaaccgg cggcgcgggc gctccgcgcg agtattgcgg
     4861 ggcgttccgt gaactacctc aggacctgaa ctcccgcacg tcgcgcgagc acccgattcc
     4921 gcgccagttc ctccagcagc actgccggaa accggtcggg atgctcttcg atggtctcga
     4981 atcccagccg catcaggccg tcgcgaaacc cggtcgaggc caccattgcc tgcaggccgt
     5041 tctgcagacg ggcgacgatg tccgccggcg ttccgcgggg taccacgatc gactgccagg
     5101 aatccgagac gacgtcggcg agaccttcct cggcaaaggt gcgcacatcg gggtaggcgc
     5161 gcgagcgcac ttcgcctgca ttcgcgatga cgcggatccg gcccgactgc acgaagggcg
     5221 cgatcaccgc cacgtcggcg accaccagat cgacctggcc cgaagcaacg tcgaggacgg
     5281 cgggcgtgaa gcccttgtag ggcacggaca ggatgttgac cccggctgcg gtcttgaagg
     5341 cctcgatcgc catcgttgcg cccggaccgg cggaggcgca ggtcagcttg cccggattcc
     5401 gtctcgcgta gtcgatcaac tgcgggacgg tgtcgatccc caggccggcg cgcgttgcca
     5461 gcacatgtgg cgtgcgcgag acgcgtgcca gcgggatgaa gtcacgcagc gcgtcgtagc
     5521 gcacgctgct gtcgaccagt ggcgccagcg tcaagccgcc gccgccggcc agaacgagcg
     5581 tatagccgtc ggaggcggcg cgggcaccgg cctcgattcc gaccgtgccg ttggcgccgg
     5641 gccggttctc gaccacgatc gaggtgtcga gctgctcgcc gagctgctgg gccacgagcc
     5701 tgccgaccag gtcgctttgt ccgcccggcg gatacgaaac gatcaggcga atcggccgcg
     5761 cgggaaatgg ctggccgagc acgggcggcg cgcccgacag caacacgcag catgccgaca
     5821 gcgcgagcag gcgtcgttgc atcaaccaac ggcggtgcaa ttgctccaat cgaagcgcct
     5881 cacgtcagac gaaatgttcg aagggcaggc gatcactcga tcgccctgcc gatccgcctg
     5941 acgatgtccc ggtgacgggc gatttccgct tgcaggatcc gtggaaacgt gtcgggcgac
     6001 tcatcgatcg gcacgaatcc gaggcgttcg aggttgtcgc gaaattccgc tgccgccagc
     6061 gcttgccgaa gcgcactttg aagtctggca atgatcccag ggggc
//
LOCUS       DBNHBY010000562         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_104704, whole genome shotgun sequence.
ACCESSION   DBNHBY010000562 DBNHBY010000000
VERSION     DBNHBY010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_104704"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="AC16HB_13750"
     CDS             <1..1571
                     /locus_tag="AC16HB_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5607898.1"
                     /translation="RNACLPAGQRIDHRIGINLGDILVDSNDIAGNGVNVAARLEAIA
                     PPGGIAVSGTVRDQVRQDLGVAFVDAGVQHVKNIRQPVRVFTVQLDGAPPLAARLRAL
                     PWRRSAVALVACLLVAAVAVAYWPRKPTAADGTLIVMPFRAAPGDENIAAALTKQLTG
                     AVSRISGLTVIAPTVADRYTGQAVDARKVGRELGVRYALEGRVERRGDSLHVTAQLVA
                     ADSGATVWTGDIDAARPADNGAPPEVIGPIADSVRSELRAAELKRLGDAESAQALALR
                     ATLDLANAMDVQELQAARGAFERALRLDPVQTLALVGLADTLAFESQRSELGPGRDAL
                     LAQADAASLRAVSADPNNAEAWGTRAQVLLFRDQIESAAQAVERALNLNPYVSDLYAL
                     RAQVLMARGEHREAVAALDRAIGLAPRGNSAGVVLNYRCRALLLLTRYDDAIEACERG
                     LAFMPDWPDYMLLTAAYAMKDDLSRARAAKAELLRLMPRFTIRWHAAVSGTARARALE
                     QTVYAGLRKAGVPE"
     gene            complement(1575..>4303)
                     /gene="hrpA"
                     /locus_tag="AC16HB_13755"
     CDS             complement(1575..>4303)
                     /gene="hrpA"
                     /locus_tag="AC16HB_13755"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR5607899.1"
                     /translation="RMSNGICIRLYGEADFNDRPRFTNPEIVRSSLASVILRMKSLRL
                     GDVRAFPFVEPPPARAIADGYDLLAELNAVDEEDDLTPIGVELARLPLDPRIGRMLLA
                     ARDNHALAEILIIAAALSVQDPRERPLESQQAADQAHRRHADERSDFLSFVKLWNFVG
                     ERLEHKKSNRKLTDDLKSNFISPRRVREWRDVHGQLHAIVGEAGWRINTSPATFEQVH
                     LSLLAGLLGNVGFKTLDADYREPPYVGARGIKFFIWPGSPLVKKAGRWVMAAELIETS
                     RLFGRCVATIEPQWIERVGHHLLKKSWTDPHWEKRAGQAMAFERAALYGMTVYAQRRV
                     PLGPRDAAQARELLIREGLVEGEIDTRGPFLVHNRKLVREIRELEHKARRQDVLVHDE
                     LIFAFYDRLLPADAYSLAQFEQWRVEAEKADPKLLFLSRDELMRHEAAGVTSDLFPKQ
                     WKMRGTTMALTYPFEPGSPRDGVTLTVPLFALNQVDPERCEWLVPGMLKEKVHLLLKS
                     LPQKLRRHCVPLPEYAVGFVDRMRHKLAEPDAPLIDALVADVRERTSVVCQAGDFKLE
                     QLPAHLTMNFKVVDEHGRQLAMGRSLPQLRAELGREAQQTFQSLASAQVAQVASERIS
                     DWDFGELPELLEVRKGGQTLIGHPALVDRGDHCTLEVFDDPIEARARHREGLRLLYRL
                     QLKEQVKFLERSLASLQTAQMQARTVPQLAAVLPSFEELRDQVVIAALDRTCLVDPWP
                     ADRASFNARRDDARGRLSLIAQEIARLVTAIVQEAAVLPKKLAALKPFPAVVADVEQQ
                     LARLFDKGFVAATAAAQLAHYPRYLKAIALRLDKLKADPARDAQRMSEVAALQTPFLR
                     ELAARKGVVDPRLEEFRWLLEELRVSLFAQELRTPMPVSVKRLAKVWESVRR"
BASE COUNT          653 a         1559 c         1436 g          655 t
ORIGIN      
        1 cgcgcaatgc ctgcctgccc gccgggcagc gcatcgacca tcgcatcggc atcaatctcg
       61 gcgacatcct ggtcgacagc aacgacatcg ccggcaacgg cgtgaacgtc gccgcacggc
      121 tcgaagcgat cgcaccgccc ggcggcattg cggtttcggg cacggtgcgc gatcaggtgc
      181 ggcaggatct cggcgtggcg ttcgtcgacg ccggcgtgca gcacgtgaag aacatccggc
      241 agcccgtccg cgtcttcacc gtgcagctcg acggcgcgcc cccgctggcc gcgcgattgc
      301 gtgcgctgcc gtggcgacgc tcggccgttg cgctcgtcgc ctgcctgctg gtcgccgcgg
      361 tcgcagtcgc ctactggccg cgcaagccga ccgctgccga cggcacgctg atcgtgatgc
      421 cgtttcgcgc agcccccggc gacgagaaca tcgcagctgc actgaccaag cagctgaccg
      481 gcgcggtctc gcgcatctcc ggactgaccg tgatcgcgcc gaccgtagcc gatcgctaca
      541 ccggccaggc ggtcgacgcc aggaaggtcg gccgcgagct cggggtccgc tatgcactcg
      601 aggggcgcgt cgagcggcgc ggcgattcgc tgcacgtcac ggcgcaactc gttgccgccg
      661 actccggggc aaccgtctgg accggcgaca tcgacgccgc acggccggcg gacaacggcg
      721 cgccgcccga ggtgatcgga ccgatcgccg acagcgtgcg ctccgagttg cgcgccgccg
      781 aactcaagcg cctcggcgac gccgaaagcg cgcaggcgct cgcgctgcgc gccacgctcg
      841 acctggccaa tgccatggac gtacaggagc tgcaggcggc gcgcggtgcc ttcgagcggg
      901 cgctgcggct cgacccggta cagacgctgg cgctggtcgg gctggccgac acgctcgcgt
      961 tcgaatcgca gcgaagcgag ctgggccccg ggcgcgacgc gctgctcgca caggcggatg
     1021 ccgcctcgct gcgcgcggtc agcgccgatc cgaacaacgc cgaagcgtgg ggcacgcgcg
     1081 cgcaggtgct gctgttccgc gatcagatcg agtccgccgc gcaggccgtc gaacgcgcgc
     1141 tgaacctcaa tccgtatgtc agcgacctgt acgcgctgcg ggcacaggtg ctgatggcgc
     1201 gcggcgagca tcgcgaggca gtggcggcgc tcgatcgcgc gatcgggctc gccccgcgcg
     1261 gcaacagcgc gggcgttgtg ctcaactacc gctgccgcgc gctgctgctg ctcacccggt
     1321 acgacgatgc aatcgaggcg tgcgagcgcg ggctggcgtt catgccggac tggcccgatt
     1381 acatgttgct caccgcggcc tatgcgatga aggacgatct ctcgcgcgcg cgggcggcca
     1441 aggctgagct actgcgcctg atgccgcgct tcacgatccg ctggcacgcc gcggtgtccg
     1501 gaaccgctcg cgcgcgcgca ctggaacaga ccgtgtacgc cggactgcgc aaagccggcg
     1561 tgccggaatg agcctcatcg ccgcaccgac tcccacacct tcgccagccg cttgacgctg
     1621 acgggcatcg gcgtacgcag ttcctgcgcg aacagcgaca cccgcagttc ctccagcagc
     1681 cagcggaatt cctccagccg gggatcgacg acccccttgc gcgcggccag ttcgcgcagg
     1741 aacggtgtct gcagcgcagc gacttcgctc atccgctgcg cgtcgcgcgc gggatcggcc
     1801 ttcagcttgt cgagccgcag cgcgatcgcc ttcagatagc gcggatagtg cgccagctgc
     1861 gccgccgccg tggccgcgac gaaacccttg tcgaacagcc gcgcgagctg ctgttccacg
     1921 tcggctacaa cggcgggaaa cggcttcagc gcggcgagct tcttcggcag cacggccgcc
     1981 tcctgcacga tcgcggtgac gagtcgcgcg atctcctgcg cgatcagcga gagcctgccg
     2041 cgcgcgtcgt cgcgccgcgc attgaagctc gcgcgatccg ccggccacgg atcgacgagg
     2101 caagtgcggt cgagcgcggc gatgacgacc tggtcgcgca gctcttcgaa gctcggcagc
     2161 accgctgcga gctgcggcac cgtacgcgcc tgcatctgtg ccgtctgcag cgacgccagg
     2221 ctcctttcga ggaacttcac ctgctccttc agctgcagcc ggtagagcag ccgcaggccc
     2281 tcgcggtgcc gcgcgcgcgc ctcaatgggg tcgtcgaaga cctccagcgt gcagtggtcg
     2341 ccgcggtcga ccagcgcggg gtgtccgatc agggtctggc cgcccttcct gacctccagc
     2401 agttcgggca gctcgccaaa atcccagtcg gaaatccgct cgctcgccac ctgcgcaacc
     2461 tgcgccgagg cgagcgactg gaatgtctgc tgcgcctcgc gcccgagctc cgcgcgcagc
     2521 tgcggcaggc tgcgtcccat cgcgagctgg cgcccatgct cgtcgacgac cttgaagttc
     2581 atcgtcaggt gtgccggcag ctgctcgagc ttgaaatccc cggcctggca gacgacgctc
     2641 gtccgctcgc gcacgtcggc gaccagcgca tcgatgagcg gtgcgtccgg ctcggcgagc
     2701 ttgtggcgca tccggtcgac gaacccaacc gcatactcgg gcagcggcac gcaatggcgg
     2761 cgcagcttct gcggcagcga cttcagcagc agatgcacct tttccttcaa catgccgggc
     2821 accagccatt cgcagcgctc cgggtcgacc tggttcagcg cgaacagcgg caccgtgagc
     2881 gtcacgccgt cgcgcgggct gccgggctcg aagggatagg tgagcgccat cgtcgtgccg
     2941 cgcatcttcc actgcttcgg aaagagatcc gaggtgacgc cggctgcctc gtgccgcatc
     3001 agctcgtcgc gcgacaggaa gagcagcttc gggtccgctt tctctgcctc gacgcgccac
     3061 tgctcgaact gcgccaacga atacgcgtcc gccggcagca gccggtcgta gaacgcgaag
     3121 atcagctcgt cgtggaccag cacgtcctgc cgccgcgcct tgtgctcgag ctcgcggatc
     3181 tcccgcacca gcttgcggtt gtgcacgagg aagggcccgc gcgtgtcgat ctcgccctcg
     3241 accaaccctt cgcggatcag cagctcgcgc gcctgcgccg catcgcgcgg acccagcggc
     3301 acgcgccgct gcgcatacac cgtcatcccg tacagcgcgg cacgctcgaa ggccatcgcc
     3361 tggccggcgc gcttctccca gtgcggatcg gtccacgact tcttcagcag gtgatgcccg
     3421 acgcgttcga tccactgcgg ctcgatcgtc gccacgcagc gcccgaacag gcgcgaggtc
     3481 tcgatcagtt ccgcagccat cacccagcgg cccgccttct taacgagcgg cgagcccggc
     3541 cagatgaaga acttgatgcc gcgcgcgccg acgtatggcg gctcgcggta atcggcgtcg
     3601 agcgtcttga agccaacgtt gccgagcaac cccgcgagca gcgacagatg cacctgctcg
     3661 aaggtcgccg ggctcgtgtt gatgcgccag cccgcctcgc ccacgatcgc gtgcagctgc
     3721 ccgtgcacgt cccgccactc ccgcacgcgc cgcggcgaga tgaagttcga cttgaggtcg
     3781 tcggtcagct tgcggttgga cttcttgtgc tcgagccgct cgccgacgaa gttccacagc
     3841 ttcacgaacg acaggaagtc ggacctctca tcggcgtgcc ggcggtgcgc ctgatcagcc
     3901 gcctgttgcg actccagcgg ccgctcgcgc ggatcctgca ccgacagcgc cgcggcaatg
     3961 atcagtatct cggccagcgc gtggttgtcg cgcgccgcga gcagcatgcg gccgatgcgc
     4021 gggtcgagcg gcagccgcgc gagctccacg ccgatcgggg tgaggtcatc ttcttcgtcg
     4081 actgcattca gttccgccag caggtcgtag ccgtcggcga ttgcgcgcgc cggcggcggc
     4141 tcgacgaacg gaaatgcgcg cacgtcgccg agtcgcagcg acttcatccg caggatcacg
     4201 cttgcgagcg acgaacgcac gatctccgga ttggtgaagc gcggccggtc gttgaagtcc
     4261 gcctcgccgt acaggcggat gcagatgccg ttgctcatcc ggc
//
LOCUS       DBNHBY010000563         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1121617, whole genome shotgun sequence.
ACCESSION   DBNHBY010000563 DBNHBY010000000
VERSION     DBNHBY010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1121617"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1450)
                     /gene="purB"
                     /locus_tag="AC16HB_13760"
     CDS             complement(77..1450)
                     /gene="purB"
                     /locus_tag="AC16HB_13760"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR5607900.1"
                     /translation="MDVSLELSALTALSPLDGRYSRITAPLRDVFSEFAFMRARLRVE
                     VEWLIGLSSLGLAELAPLPEAARIRLQTLVRDFTVADCEEIKAIEAQTNHDVKAVEYW
                     IKGRAGDVPALAAATEFVHFACTSEDINNTAHALMLAQGRVVLQDRLRALQFKLRELA
                     HAHADVPMLARTHGQPASPTTVGKEMANFAVRLAAAIDAIARVQFLAKMNGAVGNYNA
                     HLAAYPHVDWERFARGVVEGLSLSFNTHTIQIEPHDYMAELFDAIARANTVLIDLDRD
                     LWGYIALGYFKQRLKEGEVGSSTMPHKVNPIDFENSEGNLGLANALLRHLAGKLPVSR
                     WQRDLTDSTVLRNLGVAFGYCVVAYDACLRGLNKLEVNTSQLAADLDAAWEVLAEPVQ
                     TVMRRYGVPAPYEKLKELTRGRAITRDTLHQFIRALEIPDEAKQALLALTPSTYVGKA
                     GELARRC"
     gene            1634..>2147
                     /locus_tag="AC16HB_13765"
     CDS             1634..>2147
                     /locus_tag="AC16HB_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5607901.1"
                     /translation="MRLIASLTSPYVRKVRIVMAEKRIDCQIEFEDVWSPNTRTQDSN
                     PLGKVPCLIMEDGGAVFDSRVIVEYLDTMTPLGKLIPAAGRERAEVRTWEALADGILD
                     AAVLARLEQTQRPPEQQSQQWIDRQMGKVHAGLQALSRGLNDKPWCNGHGYTLADIAT
                     GCALGYLDFRF"
BASE COUNT          366 a          719 c          719 g          343 t
ORIGIN      
        1 gacgacgacg aaacgagcga cgccgcgcga ttattcccgc gccccgcgcg acgcgcatca
       61 cgcgagcagg cgcgcttcag cagcggcgcg cgagctcgcc cgccttgccg acgtaggtcg
      121 atggcgtcag cgccagcagc gcctgcttcg cttcgtcggg aatctcgagc gcgcggatga
      181 actgatgcag cgtatcgcgc gtgatcgcgc gaccccgcgt cagttccttg agcttctcgt
      241 acggcgccgg cacgccgtag cggcgcatca ccgtctgcac cggctcggcg agtacctccc
      301 acgccgcgtc gagatcggcc gccagctgcg atgtgttgac ctcgagcttg ttcagtccgc
      361 gcaggcaggc gtcgtacgcg accacgcagt agccgaacgc gacgccgaga ttgcgcagca
      421 cggtcgagtc ggtgaggtcg cgctgccagc gcgacaccgg cagtttcccg gcgaggtgtc
      481 gcagcagcgc gttggcgagc cccaggttgc cctccgaatt ctcgaagtcg atcggattga
      541 ccttgtgcgg catcgtcgac gagccgacct cgccttcctt cagcctctgc ttgaagtagc
      601 cgagcgcgat gtagccccac agatcccgat ccagatcgat caggaccgtg ttggcgcgcg
      661 cgatcgcgtc gaacagttcc gccatgtagt cgtgcggttc gatctggatc gtatgcgtgt
      721 tgaaggacag cgacagcccc tcgaccacgc cgcgcgcgaa gcgctcccag tcgacgtggg
      781 gatacgcggc caggtgcgcg ttgtagttgc cgaccgcgcc gttcatcttg gcgaggaact
      841 ggacgcgcgc gatcgcatcg atcgcggcag cgagccgcac ggcgaagttc gccatctcct
      901 tgccgaccgt ggtcggtgac gccggctggc cgtgcgtgcg cgcgagcatc ggtacgtcgg
      961 cgtgcgcgtg ggcaagctcg cgcagcttga actgcagcgc gcgcagccgg tcctgcagca
     1021 cgacgcggcc ctgcgccagc atcagcgcgt gcgcggtgtt gttgatgtcc tccgacgtgc
     1081 acgcgaagtg cacgaactcg gtcgcggccg ccagcgcggg cacgtcgccg gcgcggccct
     1141 tgatccagta ttcgaccgcc ttgacgtcgt gattggtctg cgcctcgatc gccttgatct
     1201 cttcgcagtc ggcgacggtg aagtcgcgca ccagcgtctg cagccggatg cgcgccgcct
     1261 ccggcagcgg cgccagctcc gccagaccga ggctcgacag gccgatcagc cattcgactt
     1321 ccacgcgcag ccgcgcgcgc atgaacgcga actcggagaa gacgtcgcgc agcggcgcgg
     1381 tgatccggct gtaacggccg tccagcggcg atagcgcggt cagcgccgac agttcgagcg
     1441 acacatccac gggaaaggca gcaagggtac gcaccaaggg cgtgcagcca gtctagcagc
     1501 cgctgtcgtc gtgccgcgac gcgagcgatt gcgacgaagc aatgcttgcg gttcgagtgg
     1561 cactgcgttg atatactgaa tcgcagaacg agaacaccca ccacggaagc gcgaattgaa
     1621 agtgtccaag gggttgcggc ttatcgcctc gctgaccagt ccgtacgtgc gcaaggtgcg
     1681 catcgtgatg gccgagaagc gcattgactg ccagatcgag ttcgaggacg tgtggtcgcc
     1741 caacacgcgc acccaggatt cgaatccgct cggcaaggtc ccttgcctga tcatggaaga
     1801 cggtggcgcc gtgttcgact cgcgcgtgat cgtcgagtac ctcgacacga tgacgccgct
     1861 cggcaagctg attcccgccg ccggccgcga acgcgcggag gtgcgcacgt gggaggcact
     1921 ggccgacggc atcctcgacg cggcagtgct ggcacgactg gagcagacgc agcgtccacc
     1981 ggaacagcag tcgcagcagt ggatcgaccg ccagatggga aaggtgcacg ccggcttgca
     2041 ggcgctcagc cgcggactga acgacaagcc ctggtgcaac ggccacgggt acacgctggc
     2101 ggacatcgcc accggttgcg cgctcggcta cctcgacttc cgctttc
//
LOCUS       DBNHBY010000564         5320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_578663, whole genome shotgun sequence.
ACCESSION   DBNHBY010000564 DBNHBY010000000
VERSION     DBNHBY010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_578663"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..578)
                     /locus_tag="AC16HB_13770"
     CDS             complement(162..578)
                     /locus_tag="AC16HB_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607902.1"
                     /translation="MKALRSSLAALAAGLALSTLAAPALADGRWHGGGGWHGGSRWHG
                     GVFLNFGVPFPYWGPSYYYGPPVYYGVPPAYYYGPPAVAAPASPPVYVERDEEAAPAP
                     APEPRATWWYWCDSARGYYPYVKECPGGWRRVPPQQ"
     gene            complement(707..2518)
                     /locus_tag="AC16HB_13775"
     CDS             complement(707..2518)
                     /locus_tag="AC16HB_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5607903.1"
                     /translation="MTLATLIDAELAYGDQPLLDRAQLTVLEDERIGLIGRNGTGKSS
                     LLAVLAGRVHLDGGELKLREGLNVVLVEQEPTLPHAASMHDALLLRGHVPSMHDDRER
                     WRVEARLTEFLHRLGIDGSRPLEGASGGERKRAALALAFALQPDLLLLDEPTNHLDID
                     GIERLEELVSRRGASIVITHDRRFLDRVATRIVELDRGVLRGYPGNYTAFEARRRDEV
                     DAEDAARRRFDKFWAQEEAWIRKGIEARRTRNAGRVSRLERLRSARAQRRDRIGNIKL
                     ALDAGARSGKLVAELKGVCKAFGARAIVRDLDLRVLRGDRLALIGPNGAGKSTLIRLI
                     LGDLAPDAGSVRLGTNLAVAYFDQMRAALDPGRTVAETIAPGADWIEIGGARRHVMTY
                     LADFLFPPRRANAKVAMLSGGERNRLLLARLFAQPANLLVLDEPTNDLDIESLELLEA
                     TLQDYAGTLLLVSHDRAFLDSIATQSLVAEGDGRWREYVGGYSDWIAQRPAIAAPVVA
                     PAPKSPPIARKAERIKLSFKEQRELELLPGEIEALEREQAALHASMATPDYHTRGVQQ
                     MKDDAARNEEIDTLLLQKLERWSALEEKARAAGGSPP"
     gene            2626..3009
                     /locus_tag="AC16HB_13780"
     CDS             2626..3009
                     /locus_tag="AC16HB_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607904.1"
                     /translation="MSSRFIKQAVVWFLIAIVIGIYMGASGDHRLVPVHAHFHLLGWV
                     SMTLFALIYRGWPRLSGSALATAHFWLHSLGVPLAVGSIVLILRDVAIGTPLAIAGSF
                     MILIGAICFAIRVFAVVSEQQAAAT"
     gene            3014..4990
                     /locus_tag="AC16HB_13785"
     CDS             3014..4990
                     /locus_tag="AC16HB_13785"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR5607905.1"
                     /translation="MPTAPIWTPSRERIARARITAFRGWLARERDLKFADYESMWRWS
                     VTDLEGFWSAVWQFFDVRASQPYTRLLADAKMPGAVWCPGARLNLVDQVLRHVDDRRL
                     ALVCRNELGARTEVSWRELERQVAAVAASLRALGVAPGDRVVAYLPNVPQTIVAFLAV
                     ASLGAVWSVCSPDMGPVAVLDRFRQIEPKVLIACDGYRYGGKPFDRSEIVTQLRRELT
                     TLAHTVLVPVLNEAVDAAHFPGALGWNELTARAVPLHTEQVAFDHPLWIVYSSGTTGV
                     PKAIVHGHGGILLEALKGIGLHRDLQPDDRFQWYTSTGWIMWNVQVSGLLVGATVCMY
                     DGNPGWPDPSTLWQFVADEHVTFFGAGAAFYAGCMKAGIEPARIADLSRLASIGSTGS
                     PLPAEAYRWIYAHVKRDVWLASIAGGTDLAGAFLTGLPTLPVYEGEMTCRALGLKVEA
                     YDDNGRPVLDQVGELVCSAPFPSLPLYFWNDPEGRRYRESYFDTWPGVWRHGDWVRLV
                     PRPEAIGAVIYGRSDATINRHGIRMGTAELYRAVEAIPEILDSLVVDLEYLGRPSYMP
                     LFVVLRPGCELTDELKSRIDARIRAALSARHVPDEIVAIAEVPRTLSGKKMELPVKKL
                     LLGQPVEKVLHADAMANPASLAWFVDFARRRASR"
     gene            4987..>5320
                     /locus_tag="AC16HB_13790"
     CDS             4987..>5320
                     /locus_tag="AC16HB_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR5607906.1"
                     /translation="MKRKAAPVPQSRLERLARLGLAAGELAVGGALQGLRRLAQQRSE
                     TSSSLLSAASAERLAERLARLRGAAMKLGQMLSLQGEDVLPPEFARALAVLRAEAAPM
                     PLEQVRRVL"
BASE COUNT          760 a         1824 c         1864 g          872 t
ORIGIN      
        1 cggcattgcg cgtcatcagc ctggaactcg tcgaacgact tgcggctgcc cggcagcgcg
       61 gcggtgctgg gccccgtcgg aacggtcacg cacgcggtca gcccgagtac cgctgcgcca
      121 gaggcaattt tgagcaggcg attcattgtg aaccccgtcg atcactgctg cggcggaaca
      181 cgtcgccagc cgcccgggca ttccttcacg tacgggtagt agccacgcgc gctgtcacac
      241 caataccacc aggtcgccct cggctccggt gccggcgctg gcgcagcctc ttcgtcgcgt
      301 tccacataga ccggcggcga tgccggcgcc gcgacggccg gcggtccgta gtagtaggcc
      361 gggggaactc cgtagtacac cggcggcccg tagtagtagc tcggacccca gtaggggaac
      421 ggaacgccga agttaaggaa cactccgccg tgccagcggg acccgccatg ccaaccaccg
      481 ccgccgtgcc agcgaccgtc ggcaagcgcc ggcgcggcaa gcgtcgacag cgcaagaccc
      541 gcggcgagcg ccgcgagtga ggatcgaagg gctttcatct tgacttcctc ggtacggacg
      601 accgcctcgt gcagcggtca cttccttaac gcttattgtc ggccggtgcg caccggcggc
      661 aaggcgaaaa gcgttgaagt tgtaactctt cgttgccgga cgccgctcac ggcggcgacc
      721 cgcccgcggc gcgggctttc tcctccagcg ccgaccagcg ttcgagcttc tgcagcagca
      781 acgtatcgat ctcttcgttg cgcgcggcgt cgtccttcat ctgctggacg ccgcgcgtgt
      841 ggtagtccgg cgtcgccatc gacgcgtgca gcgcagcctg ctcgcgttcg agcgcctcga
      901 tctcgcccgg caggagctcg agttcgcgct gctccttgaa ggacaacttg atccgttcgg
      961 ccttgcgggc aatgggcggg gatttcggcg cgggtgcgac cacgggcgct gcgatcgcgg
     1021 gccgctgcgc gatccagtcc gaataaccgc cgacgtattc gcgccagcgg ccgtcgccct
     1081 cggcgaccag cgactgcgtc gcaatgctgt cgaggaacgc gcgatcgtgc gacacgagca
     1141 gcagcgtgcc tgcgtagtcc tgcagcgttg cttccagcag ttcgagcgac tcgatgtcca
     1201 gatcgttcgt cggctcatcg agcacgagca ggttcgcggg ctgcgcaaag aggcgcgcca
     1261 gcagcagccg gttgcgttcg ccacccgaca acatcgcgac cttggcgttc gcgcgccggg
     1321 gcgggaacag gaagtcggcc agataggtca tcacgtggcg gcgcgcgccg ccgatctcga
     1381 tccagtccgc gcccggcgcg atcgtctcgg ccaccgtccg accgggatcg agcgccgcgc
     1441 gcatctggtc gaagtacgcg acggcgaggt tcgtgccgag ccgcacgctg ccggcatccg
     1501 gtgcgaggtc gccgaggatc agccggatca gcgtcgactt gcccgcgccg ttggggccga
     1561 tcagcgcgag ccggtcgccg cgcagcacgc gcaggtcgag gtcacgcacg atcgcgcgcg
     1621 cgccgaacgc cttgcagacg cccttcagct cggcgaccag cttgccgctg cgcgcgccgg
     1681 cgtcgagcgc aagcttgatg ttgccgatgc ggtcgcgccg ctgcgcccgc gccgaacgca
     1741 gccgttcgag gcggctcacg cgccccgcgt tgcgcgtgcg gcgcgcttcg atgcccttgc
     1801 ggatccacgc ctcctcctgc gcccagaact tgtcgaagcg gcggcgcgct gcgtcctcgg
     1861 cgtcgacctc gtcgcggcgg cgcgcctcga acgcggtgta gttgccggga tagccgcgca
     1921 gcacgccgcg gtccagttcg acgatgcgcg tggcgacgcg atcgagaaag cggcggtcgt
     1981 gcgtgatcac gatcgacgcg ccgcgtctgc tgacgagctc ttccagccgc tcgatgccgt
     2041 cgatgtcgag atgattggtc ggctcgtcga gcagcagcag gtcgggctgc agcgcgaacg
     2101 ccagcgccag cgccgcgcgt ttgcgctcgc cgcccgaggc gccttcgagc ggacgcgagc
     2161 cgtcgatacc gagacggtgc agaaactccg tgaggcgtgc ttcgacgcgc cagcgttcgc
     2221 ggtcgtcatg catcgacggg acatgcccgc gcagcagcag cgcgtcgtgc atcgacgcgg
     2281 cgtgcggcag cgtcggctcc tgctcgacca gcacgacgtt caggccctcg cgcagcttca
     2341 gctcgccgcc gtcgagatgg actcgtcccg ccagcacggc gagcagcgag ctcttgccgg
     2401 tgccgttgcg gccgatcagg ccgatccgct cgtcctcgag tacggtgagc tgcgcgcggt
     2461 cgagcagcgg ctggtcgccg taggccagtt cggcgtcgat cagggtggcg agcgtcatgg
     2521 ggcaacgata gcgaagcgcc gtagcattca caggcgtgca cgcgccgcgt atcttgcgcg
     2581 gctgcgcggc ggcgggcgtc gcgcccaaca agaagggaca caccgatgag cagtcgcttc
     2641 atcaagcagg ccgtcgtctg gtttctgatc gcgatcgtga tcggcatcta tatgggcgcg
     2701 agcggcgacc atcggctggt gccggtgcat gcgcacttcc acctgctcgg ctgggtgtcg
     2761 atgacgctgt tcgcgctgat ctaccgcggc tggccgcggc tttcgggaag cgcgctcgcc
     2821 accgcgcact tctggctgca cagcctcggc gtgccgcttg cggtcggctc gatcgtgctg
     2881 atcctgcgcg atgtcgcgat cggcaccccg ctcgccatcg ccggctcgtt catgatcctg
     2941 atcggcgcga tctgcttcgc gatccgggtc ttcgccgttg tctccgagca gcaggccgcg
     3001 gcgacctgac gcgatgccga ccgcacccat ctggactccg tcgcgcgaac gcatcgcgcg
     3061 cgctcgcatc accgcctttc gcggctggct ggcacgcgaa cgcgatctga agttcgcgga
     3121 ctacgaatcg atgtggcgct ggtcggtgac agatctcgag gggttctgga gcgcggtgtg
     3181 gcagttcttc gacgtccgcg cctcccaacc gtacacgcgc ttactcgccg acgcgaagat
     3241 gccgggcgcg gtgtggtgtc ctggcgcgcg gctgaatctg gtcgaccagg tgctgcgaca
     3301 cgtggacgac cggcggcttg ccctcgtgtg ccgcaacgag ctcggcgcgc gaaccgaagt
     3361 ctcctggcgc gagctcgagc ggcaggtcgc cgcagtggcc gcgtcgctgc gcgcgctcgg
     3421 ggtcgccccg ggggaccggg tcgtcgccta tctgcccaac gtgccacaga cgatcgtcgc
     3481 atttctcgcg gtcgccagcc ttggcgcggt ttggtcggtg tgctcgcccg acatggggcc
     3541 ggtcgcggtg ctcgaccgct tccgccagat cgagcccaag gtgctgatcg cgtgcgacgg
     3601 ctatcgctac ggcggcaagc cgttcgaccg cagcgagatc gtgacgcagc tgcgccgtga
     3661 actgacaacg ctggcgcaca ccgtgctcgt acccgtactg aacgaagcag tcgatgcggc
     3721 gcactttccc ggcgccttgg gttggaacga actcaccgcg cgcgccgtgc cgctgcacac
     3781 cgagcaggtc gcattcgatc acccgctgtg gatcgtgtac tcgtccggca cgaccggggt
     3841 gccgaaggcg atcgtgcatg gccacggcgg cattctgctc gaggcgctga agggcatcgg
     3901 cctgcatcgc gacctgcagc ccgacgaccg cttccagtgg tacacgagca ccggctggat
     3961 catgtggaac gtgcaggtca gcggtctgct ggtcggcgcg accgtctgca tgtacgacgg
     4021 caatccgggc tggccggatc catcgacgct gtggcagttc gtcgccgacg agcacgtcac
     4081 gttcttcggt gccggcgccg cgttctatgc gggctgcatg aaggccggca tcgagccggc
     4141 gcgcatcgcc gatctgtcgc ggctcgccag catcggctcg accggatcgc cgctgccggc
     4201 ggaggcgtat cgctggatct acgcgcatgt gaagcgcgac gtatggctcg cttcgatcgc
     4261 cggcggcacc gatctggcag gcgcgttcct gacggggctg ccgacgctgc ccgtgtacga
     4321 gggcgagatg acctgccgtg cgctcgggct caaggtcgag gcatacgacg acaatggccg
     4381 gccggtgctc gaccaggtcg gcgaactcgt atgcagcgca ccgtttccgt cgctgccgct
     4441 ctacttctgg aacgaccccg agggccgccg ctatcgcgaa agctatttcg acacctggcc
     4501 cggcgtgtgg cgacacggcg actgggtgcg gctggtgccg cggcccgagg cgatcggggc
     4561 cgtcatctac ggccgctccg acgcgacgat caatcggcac ggcatccgca tgggcaccgc
     4621 ggagctgtat cgcgcggtcg aggcgattcc cgagatcctc gattcgctcg tggtcgatct
     4681 cgaatatctt ggccgcccgt cgtacatgcc gctgttcgtc gtgctgcggc cgggctgcga
     4741 gctgaccgac gagctgaagt cacgcatcga cgcgcgcatc cgcgcggcgc tgtcggcgcg
     4801 ccacgtgccc gacgagatcg tcgcgatcgc cgaggtcccg cgcacgctgt cgggcaagaa
     4861 gatggagttg ccggtgaaga agctgctgct cggccagccg gtggagaagg tgttacacgc
     4921 cgatgcgatg gcaaacccgg cgagcctggc gtggtttgtc gacttcgcgc gccggcgcgc
     4981 ttcacggtga agcgcaaggc ggcgccggtc ccgcagagcc ggctcgagcg actggcgcgg
     5041 cttggcctcg cggcgggcga actcgccgtc ggcggcgcgc tccaggggct gcggcggctc
     5101 gcacaacagc gctcggagac atcgtcctcg ctgctgtcgg ccgccagtgc ggagcgcctg
     5161 gccgagcggc ttgcgcggtt gcgcggcgcc gcaatgaagc tcgggcagat gctgtcgctg
     5221 cagggcgagg acgtgctgcc gccggaattc gcgcgcgcgc tcgcggtgct gcgcgcggag
     5281 gcggcgccga tgccgctcga gcaggtgcgg cgcgtgctgg
//
LOCUS       DBNHBY010000565         3963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_782981, whole genome shotgun sequence.
ACCESSION   DBNHBY010000565 DBNHBY010000000
VERSION     DBNHBY010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_782981"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="dnaX"
                     /locus_tag="AC16HB_13795"
     CDS             complement(<1..1560)
                     /gene="dnaX"
                     /locus_tag="AC16HB_13795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR5607907.1"
                     /translation="MSYQVLARKWRPRDFASLVGQDHVVRALTHALEQKRIHHAYLFT
                     GTRGVGKTTLSRILAKALNCVGPDGSGGITATPCNRCPVCEAIDAGRFVDYIEMDAAS
                     NRGVDEMTALLERAVYAPSNARFKVYMIDEVHMLTTHAFNAMLKTLEEPPDYVKFILA
                     TTDPQKLPATVLSRCLQFNLKQMAPAAVAALMADILAREGISAEPAALKMLGHAARGS
                     MRDGLSLLDQAIAYSAGNVTLDAVRNMLGAIDTTTLVRVLDAVAARDAKQALAVADEM
                     AARSFSFAQALRDLAGLLHWIALAQQVPEAIGDEVENAADVLRFAQSIAPDEVQLLYQ
                     IALHGRNDLHLAPDEYAGFTMTLLRMIAFAPADAATEAPPRAVPLAASKPRTDAPAAP
                     APVAVANVAFDGNWPALVERLKVGGMVRELATRSELVAAEADHFKLRVPIRTLAEGSG
                     LEKLKAALAQHFGRTIRVTVEVGATAGLTAAGIADQARAEKQKRAEEAIYADPFVKEL
                     IENFGATVDPAS"
     gene            1648..3183
                     /locus_tag="AC16HB_13800"
     CDS             1648..3183
                     /locus_tag="AC16HB_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIR5607908.1"
                     /translation="MDTIAGTASRLIAHAVRLAVFLCTAVVAATANADDTLRIGSKRF
                     TEAYILAQVLAQTAQTAGTKVEVKAGLGNTAIVYEALRSGAIDLYPDYTGTIDLEILK
                     NPQPTSLDAMNRQLAPLGFGVAVPLGFNNGYALALRAADAERLGIKSLSDLAQHPELK
                     LGLSNEFIGRADGWRPLAQRYGLPHQPVGLDHGIAFDAIGKGQIDVTDIYTTDAQIEK
                     MKLRVLADDRNYFPRYEAVLLYRLDVPQRFPQAWAALQKLAGTIDERTMIAMNARAEL
                     QSAPFEQIARDHLAGRDRPTGGQRGFWAKLAGPDLGRLTLQHLWLVAASVVAAALIGI
                     PLALLVAPHPRARAGVLGATGLLQTIPSLALLAALISWLGIIGTVPALIALTLYALLP
                     IMRNTCTGVAEIPAGLIDAARALGLSNGQRLRLIELPLAAPTIIAGVRIAAVIAVGTA
                     TIAAFIGAGGYGERIVTGLALNDGALLLAGALPAALMAVACELLFEAIESAVRRGRGI
                     ETP"
     gene            3180..>3963
                     /locus_tag="AC16HB_13805"
     CDS             3180..>3963
                     /locus_tag="AC16HB_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR5607909.1"
                     /translation="MTGELNFLTACELGARIRRREISALDVMRAHISQIERVNPKVNA
                     IVTFHSERALAQARAADERQARGERLGVLHGLPTAHKDLVPTQGVRTTWGSPIYKDFV
                     PGEDALIVQRQRDAGAISVGKTNTPEFGAGSQTFNAVFGATRNPYDLARTCGGSSGGA
                     AVALACGMVPIADGSDLGGSLRNPASFCNVVGFRASAGRVPKYPSKLAWNTLSVEGAM
                     ARTVEDVALMMQAISGPDARVPISLTDAGAIFAQPLERDFAGT"
BASE COUNT          598 a         1424 c         1325 g          616 t
ORIGIN      
        1 cgacgccggg tcgacggtgg cgccgaaatt ctcgatcagc tctttgacga acggatcggc
       61 atagatcgcc tcctccgcgc gcttctgctt ctcggcgcgc gcctgatcgg caatgccggc
      121 cgcggtcagg cctgcggtcg cgccgacctc gaccgtcacg cggatcgtgc ggccgaagtg
      181 ctgcgcgagt gcggccttca gcttctccag cccactgcct tcggccagcg ttctgatcgg
      241 cacgcgcagc ttgaagtgat ctgcctcggc ggcgaccagt tcgctccgcg tcgccagctc
      301 gcgcaccatg ccgcccacct tcagacgctc gaccagcgcc ggccagttgc cgtcgaaggc
      361 gacgttggcc accgcgaccg gagcgggcgc cgcaggtgca tcagtgcgcg gcttgctcgc
      421 cgccagcggc acggcgcgcg gcggtgcctc cgtcgctgcg tcggccggcg cgaatgcgat
      481 catgcgcagc agcgtcatcg tgaagccggc atattcgtcg ggcgccagat gcaggtcgtt
      541 gcgcccgtgc agcgcaatct gatagagcag ctgaacttcg tccggcgcaa tgctctgcgc
      601 gaaccgcagc acatcggccg cattctcgac ttcgtcgccg atcgcctccg gcacctgctg
      661 cgccagcgcg atccagtgca gcagtcctgc cagatcgcgc agcgcctgcg cgaacgagaa
      721 gctgcgcgcg gccatctcgt cggcgaccgc cagcgcctgt ttcgcgtcac gcgccgcgac
      781 cgcatcgagc acgcgcacca gcgtcgtggt gtcgatcgcg cccagcatgt tgcgcaccgc
      841 atccagcgtc acgttgccgg cgctgtaggc gatcgcctgg tccagcagcg acaagccgtc
      901 gcgcatgctg ccgcgcgcgg catggccgag catcttcagc gccgccggct cggcgctgat
      961 gccttcccgg gcgaggatgt cggccatcag cgcggccacc gccgccggcg ccatctgctt
     1021 caggttgaac tgcaggcagc ggctgaggac ggtcgccggc agcttctgcg gatcggtggt
     1081 ggcgaggatg aacttgacgt agtcaggcgg ctcctccagc gtcttgagca tcgcgttgaa
     1141 cgcgtgcgtg gtgagcatgt gcacttcgtc gatcatgtag accttgaagc gcgcattgct
     1201 cggcgcgtac accgcgcgtt ccagcagcgc ggtcatctcg tccacgccgc ggttgctggc
     1261 ggcgtccatc tcgatgtagt cgacgaagcg gccggcgtcg atcgcttcgc acaccgggca
     1321 gcgattgcag ggagtcgccg tgatgccgcc gctgccatcc ggcccgacgc agttcagagc
     1381 cttggcgagt atgcgcgata gcgtggtctt gcccacgccg cgcgtgccgg tgaagagata
     1441 cgcgtggtgg atccgcttct gctcgagcgc gtgcgtgagc gcgcgcacca cgtggtcctg
     1501 cccgaccagc gaggcgaaat cacgcgggcg ccacttgcgt gcgagtacct gatacgacat
     1561 gcaaatgatt ctacctacgc gcaggagtgc ttcccggcgg cgcgctgcgg agcgatgatc
     1621 gcgcctcgcc aatcaagcga gatggccatg gacacgattg ccggcaccgc gagtcgactg
     1681 atcgcgcacg cagttcggct cgcggtcttc ctttgcaccg cggtcgtcgc ggccacggcg
     1741 aacgcggacg acacgctgcg catcggctcc aagcgcttca ccgaggccta catcctggcg
     1801 caagtcctcg cgcagaccgc gcagaccgca ggcacgaagg tcgaagtcaa ggccgggctc
     1861 ggcaacaccg cgatcgtcta cgaggcgctg cgctcgggcg cgatcgacct ctatcccgac
     1921 tacaccggca cgatcgacct ggaaatcctg aagaaccccc agccgacgtc gctcgacgcg
     1981 atgaaccgcc agctcgcgcc gctcggattc ggcgtcgcag taccgctcgg cttcaacaac
     2041 ggctacgcac tggcactgcg cgcggccgac gccgagcggc tcggcatcaa gtcgctcagc
     2101 gatctcgcgc aacaccccga gttgaagctg ggcctgtcca acgaattcat cggccgcgcc
     2161 gacggctggc gcccgttggc gcagcgctac gggctgccgc accaaccggt cggactcgac
     2221 cacggcattg cattcgacgc gatcggcaag gggcagatcg acgtgacgga catctacacc
     2281 accgacgcgc agatcgagaa gatgaagttg cgcgtgctgg ccgatgaccg caactacttc
     2341 ccgcgctatg aggcggtgct gctgtatcgg ctcgacgtgc cgcagcgttt cccgcaggcg
     2401 tgggcggcgc tgcagaagct cgccggcacg atcgacgagc gcacgatgat cgcgatgaac
     2461 gcgcgcgccg aactgcagag cgcgccgttc gaacaaatcg cgcgcgatca cctcgcgggc
     2521 cgggaccggc ccaccggggg ccagcgcggc ttctgggcca agctcgccgg ccccgacctc
     2581 ggccgcctga cactgcagca cctgtggctc gtcgccgcgt cggtcgtcgc ggcggcactg
     2641 atcggcattc cgctcgcgct gctcgtcgcg ccccatccgc gcgcccgcgc gggcgtgctc
     2701 ggcgcgaccg ggctgctgca gacgattccc tcgctcgcgc tgctggccgc gctgatttcc
     2761 tggctcggca tcattggcac cgtgccggcg ctgatcgcgc tgaccctgta cgcactgctg
     2821 ccgatcatgc gcaacacctg caccggggtg gccgagattc ccgccgggct gatcgacgct
     2881 gcgcgcgcgc tcggtctgtc caacgggcag cggctgcgac tgatcgaact gccgctcgcg
     2941 gctccgacga tcatcgccgg cgtgcgcatc gccgccgtga tcgcggtcgg cacggcgacg
     3001 atcgccgcat tcatcggcgc cggcggctac ggcgagcgca tcgtcaccgg gctggcgctg
     3061 aacgacggcg cgctgctgct cgcaggcgcg ctgcccgccg cactgatggc ggtggcgtgc
     3121 gagctgctgt ttgaagcgat tgaatccgcg gtccgccgcg gccgaggcat cgaaacgcca
     3181 tgaccggcga actgaacttc ctcaccgcct gcgaactcgg cgcgcgcatc cgccgccgcg
     3241 agatctccgc actcgatgtc atgcgggcac acatctcgca gatcgagcgc gtcaacccca
     3301 aggtcaatgc gatcgtgacc ttccactccg agcgcgcgct ggcgcaggcg cgcgcggccg
     3361 acgagcggca ggcgcgcggg gagcggctcg gcgtgctgca cggcctgccg accgcgcaca
     3421 aggacctggt cccgacccag ggcgtgcgca cgacctgggg ttcgccgatc tacaaggact
     3481 tcgtacccgg cgaggacgcg ctgatcgtcc agcggcagcg cgacgcgggc gcgatctcgg
     3541 tcggcaagac caacacgccc gagttcggcg ccggctcgca gacgttcaac gcggtgttcg
     3601 gcgccacgcg caacccgtac gacctcgcgc gcacctgcgg cgggtcgtcc ggcggcgcgg
     3661 cggtcgcact cgcctgtggc atggtcccga tcgccgacgg cagcgatctc ggtggcagcc
     3721 tgcgcaatcc ggcgagcttc tgcaacgtgg tcggctttcg cgcttcggcc ggccgcgtgc
     3781 ccaagtaccc gtccaagctc gcctggaaca cgctatcggt cgagggcgcc atggcacgca
     3841 cggtcgagga cgtggcgctg atgatgcagg cgatcagcgg gcccgatgca cgcgtgccga
     3901 tctcgctcac cgacgcggga gcgatcttcg cgcagccgct cgagcgcgac tttgccggca
     3961 cgc
//
LOCUS       DBNHBY010000566         4657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_646383, whole genome shotgun sequence.
ACCESSION   DBNHBY010000566 DBNHBY010000000
VERSION     DBNHBY010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_646383"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="AC16HB_13810"
     CDS             complement(<1..683)
                     /locus_tag="AC16HB_13810"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5607910.1"
                     /translation="MKTIGSIRRWLVLLVLALAAGGAFAQGASPPFDHLRTGFALTGE
                     HARVRCESCHLNGVFKGTPRDCASCHLSGMRFARSNVVMPPQHPPTQASCDTCHTTTT
                     FVGAKFSHAGVAAGTCQTCHNGKIAPARSAQHMPTGSAQSCDECHKSTSAWRPTAWNH
                     TQLAVAGQCASCHSGAYPPADGRPANHVPYQTIASISSANCDSCHKGSYASWANGRVH
                     ANVSVAGGCA"
     gene            complement(680..2524)
                     /locus_tag="AC16HB_13815"
     CDS             complement(680..2524)
                     /locus_tag="AC16HB_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR5607911.1"
                     /translation="MRARCAIRWLHVFALACLASLAQPAAAQSFESVLRPGELIQGHA
                     KWEEECGKCHVRFDRAAQDRLCMDCHKEVGRDVREHTGHHGRLKPQPCRACHTDHKGR
                     GARIVELDRKTFDHAQTDFALRGKHRDTECDKCHEPKKKYWQASLDCQSCHRKDDKHK
                     GALGPKCADCHTESNWKESRFDHEKTRFALTGKHVDVKCDDCHRDARYRDIPRTCIGC
                     HRTDDDRKGHKGLFGEKCESCHGTKGWKPSIFNHDADTKYPLRGRHRSAKCADCHTGY
                     LYKEKTSTACADCHRKDDKHKGTLGRECGACHTERDWKETGRFDHAKTDFALLGKHAD
                     VKCAQCHKSAVYNEAPKDCYSCHRKDDRHRGNVGPQCQDCHVERDWKTVDRFDHGRTK
                     FPLRNAHATRKVQCTDCHADPARYRGTPLDCLSCHRKDDKHEGQQGAKCDSCHDDRDW
                     KTTRFDHRQSRFPLLGRHVAVACKDCHATPRYKDTPRDCHSYHRKDDKHKLKFGTACE
                     NCHNARAWGVWDYDHARRARYALDGAHAKLACESCHTRPAPAGRNAADVGTGCIGCHR
                     RDDKHDGAFGAACERCHATDDWKRIRNRIGAALGYSSRFALVAREALR"
     gene            complement(2542..3447)
                     /locus_tag="AC16HB_13820"
     CDS             complement(2542..3447)
                     /locus_tag="AC16HB_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607912.1"
                     /translation="MKAASREVLAAGNAPRRVRTGTPRVRSSDGSLYLLLGALVFAAW
                     QLSRRGFVTAGSDVGYWLGVGGGVMMLALFLYPLRKHVHFMRALGPAKYWFVFHMVCG
                     IGGPLLILVHCAFRIGSLNAAVALFSMLTVAASGIVGRFIYAHIHRGLLGEKSSLREV
                     QAAAGFQHDAVKTRFHFAPTVEQRLFAFEADALRSEGGWRNDLRRLFALPYRQWRTYH
                     ACARELDQIVVKLGIERSWRRGDLNDRRHRARALLRAYLASVVRVAQFSVYERLFALW
                     HVLHIPFVYVMLITAVVHVVAVHAY"
     gene            3715..>4657
                     /locus_tag="AC16HB_13825"
     CDS             3715..>4657
                     /locus_tag="AC16HB_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5607913.1"
                     /translation="MTETLSLLPYLALILIAVAFYVLRRRRLERAHAETLQEAIESGL
                     TEPPSLHPVVDPVRCVGSGGCVRACPEQALGLINGKATLVNASACIGHGACAAACPLE
                     AIRLVFGTERRGIDIPQVKPNFESNVPGIFIAGELGGMGLIRKAAEQGSRAIDAIRAR
                     GGAGSDLDVVIVGAGPAGLSAGLAATEHGLHYRILEQEHSLGGAIFHYPRNKIAMTAP
                     VKLPIVGKVKLGEISKEKLLDFWQRAVDASGLQIQFGERMETIERRDGGFVVHTTKRK
                     YTAGSVLLAIGRRGTPRKLGVPGEESAKVVYRLVDPEQ"
BASE COUNT          715 a         1582 c         1561 g          799 t
ORIGIN      
        1 gcgcagccgc ccgcaacgct gacattggcg tgcacgcgac cattcgccca cgacgcatag
       61 ctgcccttgt ggcaggaatc gcaattcgcc gacgagatcg acgcgatcgt ctggtacggc
      121 acgtggttgg ccggcctgcc gtcggccggc ggatatgcac ccgagtggca ggacgcgcat
      181 tgcccggcaa ccgcgagctg cgtgtggttc cacgcggtcg gccgccatgc gctcgtcgac
      241 ttgtggcatt cgtcgcagga ctgcgccgat ccggtcggca tgtgctgcgc tgacctggca
      301 ggcgcgatct tgccgttgtg gcacgtctgg caggtgcccg cggccacgcc cgcatgcgag
      361 aacttcgcgc cgacgaaggt cgtcgtggtg tggcaggtgt cgcagctcgc ctgcgttggc
      421 ggatgctgcg gcggcatcac cacgttggac cgcgcgaacc gcatgcccga caggtggcac
      481 gacgcgcagt cgcgcggcgt acccttgaag acgccgttga ggtgacacga ctcgcagcgc
      541 acgcgcgcgt gctcgcccgt cagcgcgaat cccgtccgca ggtggtcgaa cggcggcgag
      601 gcgccctgcg cgaacgcgcc gcccgccgcg agcgcgagca cgagcagcac gagccagcgc
      661 cggatcgatc cgatcgtttt catcgcagcg cctcccgtgc cacgagtgca aaccggctcg
      721 agtaaccgag cgcagcgccg atgcgattgc ggatgcgctt ccagtcatcc gtcgcatggc
      781 agcgctcgca ggcggcgccg aacgcgccgt cgtgcttgtc gtcgcggcga tggcagccga
      841 tgcagccggt accgacgtcc gccgcattcc gccccgccgg cgccggccgc gtgtggcaac
      901 tttcgcaggc gagcttcgcg tgcgcgccat cgagcgcgta cctggcgcgc cgcgcgtggt
      961 cgtagtccca gacgccccag gcgcgcgcgt tgtggcagtt ctcgcaggcg gtgccgaact
     1021 tcagcttgtg cttgtcgtcc ttccggtggt aactgtggca atcgcgcggc gtgtccttgt
     1081 agcgcggtgt ggcgtggcag tccttgcagg cgaccgcgac gtggcgaccg agcaggggga
     1141 agcgcgactg ccgatgatcg aagcgcgtcg tcttccaatc gcggtcgtcg tggcacgagt
     1201 cgcacttcgc accctgctgg ccctcgtgct tgtcgtcctt ccggtggcac gacaggcagt
     1261 cgagcggtgt gccgcgatag cgcgccggat ccgcgtggca gtcggtgcac tgcacctttc
     1321 gcgtcgcgtg cgcgttgcgc agcggaaact tcgtccgacc gtgatcgaag cggtcaacgg
     1381 tcttccagtc gcgctcgacg tggcagtcct ggcactgcgg gccgacgttg ccgcggtgcc
     1441 gatcgtcctt cctgtggcag gagtagcagt ccttcggcgc ctcattgtaa acggcgctct
     1501 tgtggcactg cgcacacttc acgtcggcat gcttgccgag cagcgcgaag tcggtcttcg
     1561 cgtgatcgaa ccggccggtt tccttccagt cgcgctcggt gtggcaggcg ccgcactcgc
     1621 gcccgagcgt gcccttgtgc ttgtcgtcct tgcgatgaca gtcggcgcac gcggtcgagg
     1681 tcttctcctt gtagaggtac ccggtgtggc agtccgcgca cttcgcgctg cgatgcctgc
     1741 cgcgcagcgg gtacttcgtg tccgcgtcgt gattgaagat cgacggcttc catcccttcg
     1801 tgccgtggca gctttcgcac ttctcgccga acagaccctt gtgccccttg cggtcgtcgt
     1861 ccgtacgatg gcagccgatg caggtgcgcg ggatgtcgcg gtacctggcg tcgcggtggc
     1921 agtcgtcgca cttgacgtcg acatgtttgc cggtcagcgc aaagcgcgtt ttctcgtggt
     1981 cgaaccgcga ctccttccag ttgctctccg tgtggcagtc ggcgcacttc ggccccagcg
     2041 cgcccttgtg cttgtcgtcc ttgcggtggc agctctggca gtcgagcgac gcctgccagt
     2101 acttcttctt cggctcgtgg cacttgtcgc actcggtgtc gcggtgcttg ccgcgcagcg
     2161 caaagtccgt ttgcgcgtga tcgaatgtct tccgatccag ctcgacgatg cgcgcgccgc
     2221 gacccttgtg atcggtgtgg cacgcccggc acggctgcgg cttcaggcgc ccgtgatggc
     2281 cggtgtgctc acgcacgtcg cgccccactt ccttgtggca atccatgcag agccggtcct
     2341 gcgcggcgcg gtcgaagcgc acatggcact tgccgcattc ctcttcccac ttggcgtgcc
     2401 cctggatcag ttcgcccggg cgcagcaccg actcgaacga ttgcgccgct gccggctgcg
     2461 cgaggctcgc cagacatgcc agcgcgaaca cgtgcagcca tcggatcgcg catcgcgctc
     2521 gcaccgggtt cgctccggcg ctcagtaggc atgcacggcg acgacatgga ccaccgcggt
     2581 gatcagcatc acgtacacga acgggatgtg cagtacatgc cagagcgcga acagccgctc
     2641 gtagacgctg aactgcgcca cccgcaccac gctcgcgagg taggcgcgca gcagggcgcg
     2701 cgcgcggtgg cggcggtcgt tcaggtcacc gcggcgccag ctgcgctcga tgccgagctt
     2761 cacgacgatc tggtcgagct cccgcgcgca ggcgtggtac gtgcgccact ggcggtacgg
     2821 caatgcgaac agccggcgca ggtcgttgcg ccatccgcct tcactgcgca gcgcgtcggc
     2881 ctcgaacgca aacagccgct gctcgaccgt gggcgcgaag tggaagcgcg tcttgaccgc
     2941 gtcgtgctgg aatccggctg ccgcttgcac ctcgcgcaga ctgctcttct cgcccagcag
     3001 cccgcggtgg atgtgggcgt agatgaagcg gccgacgatc ccgctcgcgg caacggtcaa
     3061 catcgagaac agcgcgaccg ctgcgttcag cgaaccgatg cgaaacgcgc aatgcacgag
     3121 aatcagcagc ggcccgccga tgccgcacac catgtggaac acgaaccagt atttggccgg
     3181 cccgagcgcg cgcatgaagt gcacgtgctt gcgcagcgga tagaggaaca gcgcgagcat
     3241 catcacgccg ccccccacac cgagccagta accgacgtcc gagcccgcag tgacgaatcc
     3301 gcgccgactc agttgccacg cggcgaagac cagcgcgcct agcagcagat acagcgagcc
     3361 atcgctcgat cggacacgtg gtgtgccggt acgaacgcgg cgaggcgcgt tgcccgccgc
     3421 gagaacctct cgacttgccg ccttcatcac tggggtctgt tcgcttcttg tgttcgacgc
     3481 attcgtcgga acaccgcgcc gttgcggccg cggtgccacg gctgactcga cgcggagtga
     3541 ccggttgacc gcgcgagatc cgacgtgccg aagatagcga catgacgcga atcccaatat
     3601 cgaacgaagg gtctatctca ttgacgggaa tgaatttgtc cgttcgaggc gaatttgggc
     3661 tcgcgatgaa aatgcgagtc acacaaaaac ggctattttc agaaccggca caaaatgacc
     3721 gaaacgctct ccctcctgcc ctaccttgcg ctcatcctga tcgcggtcgc gttctacgtg
     3781 ctgcgccggc gtcggctcga acgcgcgcac gccgagacac tgcaggaggc gatcgaatcc
     3841 gggctcaccg agccgccgtc gctgcacccg gtcgtcgatc cggtgcgctg cgtcgggtcc
     3901 ggtggctgcg tgcgtgcgtg ccccgagcag gcgctcggtc tgatcaatgg caaggccacg
     3961 ctggtgaacg cgtcggcgtg catcggtcac ggcgcgtgcg ccgcggcctg tccgctcgag
     4021 gcgatcaggc tcgtcttcgg caccgagcgc cgcggcatcg acatcccgca ggtgaagccg
     4081 aacttcgaga gcaacgtgcc gggcatcttc attgccggcg aactcggcgg catggggttg
     4141 atccgcaagg ccgccgagca gggcagccgg gcgatcgacg cgatccgcgc gcgcggcggc
     4201 gcgggcagcg acctcgacgt ggtgatcgtc ggcgccggcc cggccgggct gtccgccgga
     4261 ctcgcggcca cggaacatgg gctgcactac cggatcctcg aacaggagca ttcgctcggc
     4321 ggcgcgatct tccactaccc gcgcaacaag atcgcgatga ccgcgccggt gaagctcccc
     4381 atcgtcggca aggtcaagct cggcgagatc agcaaggaaa agctgctcga cttctggcag
     4441 cgcgccgtcg atgccagcgg cctgcaaatc cagttcggcg agcgcatgga gacgatcgag
     4501 cgtcgcgacg gcggcttcgt cgtgcacaca accaagcgca agtacacggc cggctcggtg
     4561 ctgctggcga tcggccggcg cggcacgccg cgcaagctcg gcgtgccggg cgaggagtcc
     4621 gcgaaggtcg tctaccggct ggtcgatccc gagcagt
//
LOCUS       DBNHBY010000567         2037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_104868, whole genome shotgun sequence.
ACCESSION   DBNHBY010000567 DBNHBY010000000
VERSION     DBNHBY010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_104868"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="AC16HB_13830"
     CDS             complement(<1..836)
                     /locus_tag="AC16HB_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5607914.1"
                     /translation="MSTPLVEVRVPDIGNFKDVEIIDVLVKSGDTIAVDTPLITLETE
                     KATMDVPSTAAGVVREVAVQRGGRVSQGDLIIKVEAGEAPQEPAQAAAAQGTKSPAPA
                     AAAATAPLPASAAAAPSTPKPAPVAAPPSAAAPATASATQTFDEPGFSRAHASPSIRK
                     LARELGVDLAKVAGTGTKGRITDADVKAYVKRALSGGAAAQGAVGAGLPRVPPVDFAA
                     FGPIERRPLSRIQKISGPRLHASWVNLPHVTQFDEADITAMEQARSNMKTQATERGIK
                     LTP"
     gene            complement(857..>2037)
                     /locus_tag="AC16HB_13835"
     CDS             complement(857..>2037)
                     /locus_tag="AC16HB_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR5607915.1"
                     /translation="TMAFVRLLGMLLKDKQIGPHVVPIIPDEARTFGMEGMFRQIGIY
                     SSMGQLYTPQDADQLLYYREDKKGQILEEGINEGGATCSWIAAGTAYANHGVNMVPFY
                     IYYSMFGFQRVGDFCWAAGDLRTRGFLIGGTAGRTTLAGEGLQHQDGHSQLVATTIPN
                     CVAYDPTFAYELAVIIQDGMRRMYAERESVFYYITCMNENYAHPAMPEGAAPGILKGL
                     YLFRRGGKGKVRANLLGSGTILREVIKAADLLEKEFGVAADVFSAPSFSELRREALDA
                     ERWNLLHPEEAPRVPYVRQCLEGHPGPFVAATDYMRTVPDQIRQWVPGAYHVLGTDGY
                     GRSDSRAALRAFFEVDHRYVALAALRALADEGKLDRGTLRKAIERFGIDPAKPNPVTV
                     "
BASE COUNT          286 a          709 c          704 g          338 t
ORIGIN      
        1 ggcgtgagct tgatgccgcg ctcggtcgcc tgcgtcttca tgttcgagcg ggcctgctcc
       61 atcgctgtga tgtcggcctc gtcgaactgc gtgacgtgcg gcaggttgac ccagctcgcg
      121 tgcaggcgcg ggccggaaat cttctggatg cgcgacaggg gtcgccgttc gatcgggccg
      181 aacgccgcga aatccaccgg cggcacgcgc ggcaggcccg cgccgaccgc gccttgcgca
      241 gccgccccac cgctcagcgc gcgcttgacg tatgccttga cgtcggcgtc ggtgatgcgg
      301 cctttcgtgc cggtgcccgc caccttcgcg aggtcgacgc cgagctcgcg cgcgagcttg
      361 cggatcgacg gactcgcgtg ggcgcgcgag aagcccggct cgtcgaacgt ctgtgtcgcc
      421 gatgctgtag cgggagcggc agccgaaggt ggcgccgcga ccggtgcagg cttcggcgtg
      481 ctcggcgcag ctgccgctga ggccggcagt ggcgcggtgg ccgccgcggc ggccggcgca
      541 ggggatttcg tgccctgagc agccgccgct tgcgcaggct cctgtggcgc ctcgcccgcc
      601 tcgaccttga tgatgaggtc gccctgcgac acgcgcccac cgcgctgcac ggcgacctcg
      661 cgcacgacgc cggccgcggt cgacggcacg tccatcgtcg ccttctcggt ctcgagcgtg
      721 atcagcggcg tgtccacggc gatcgtgtcg ccggacttca ccagcacgtc gatgatctcg
      781 acgtccttga aattgccgat gtcagggacg cggacttcga ccagcggagt gctcatcgct
      841 gctgcctcgc ccgcgctcag accgtcaccg ggttcggctt ggccggatcg atgccgaagc
      901 gctcgatggc cttacgcagc gtgccgcggt ccagcttgcc ctcgtcagcg agcgcacgca
      961 gcgccgcgag cgcgacgtag cgatgatcca cttcgaagaa cgcgcgcaac gccgcgcgcg
     1021 aatcgctgcg gccgtaacca tccgtgccca gcacgtggta tgcgcccggc acccactgcc
     1081 ggatctggtc gggcacggtg cgcatgtagt cggtcgccgc gacgaaaggc cccgggtggc
     1141 cttccaggca ctggcgcacg tacgggacgc gcggcgcctc ttccgggtgc agcaggttcc
     1201 agcgctcggc gtcgagggcc tcgcgccgca gctcggagaa gctcggcgcg ctgaagacgt
     1261 cggctgcgac accgaactct ttctcgagca ggtcggcggc cttgatgacc tcgcgcagga
     1321 tcgtgccgga gccgagcagg ttggcgcgaa ccttgccctt gccgccgcgc cggaacaggt
     1381 acagcccctt caggatgccc ggcgccgcgc cctccggcat cgccggatgc gcgtagttct
     1441 cgttcatgca ggtgatgtaa tagaagaccg actcgcgctc cgcatacatg cgccgcatgc
     1501 cgtcctggat gatcacggcc agctcgtagg cgaacgtcgg atcgtaagcg acgcagttcg
     1561 ggatcgtcgt ggcgaccagc tggctgtgcc cgtcctggtg ctgcaggcct tcgccggcca
     1621 gcgtcgtgcg gccggccgtg ccgccgatca ggaagccgcg cgtgcgcaga tcgcccgccg
     1681 cccagcagaa gtcgccgacg cgctggaagc cgaacatcga atagtagatg tagaacggca
     1741 ccatgttgac gccgtggttc gcgtacgccg tgccggccgc gatccagctg cacgtggcgc
     1801 caccttcgtt gatgccctct tcgaggatct ggcccttctt gtcctcgcgg tagtagagca
     1861 gctggtccgc gtcctgcggc gtgtacagct gccccatgga cgagtagatg ccgatctggc
     1921 ggaacatgcc ctccatgccg aaggtgcgcg cctcgtcggg gatgatcggc acgacgtgcg
     1981 ggccgatctg cttgtccttg agcagcatgc cgagcagccg cacgaacgcc atcgtcg
//
LOCUS       DBNHBY010000568         5118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_37543, whole genome shotgun sequence.
ACCESSION   DBNHBY010000568 DBNHBY010000000
VERSION     DBNHBY010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_37543"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="AC16HB_13840"
     CDS             complement(<1..354)
                     /locus_tag="AC16HB_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HIR5607916.1"
                     /translation="MLLHFEDEATFAQQLAAATGLAPQRIARHRFPDGELRLTLPQAL
                     PPRVVALRSLHQPNEKLIELMLVARTARELGARELVLVAPYLGYMRQDIAFSPGEAVS
                     QRIVGKFLASLFDTVI"
     gene            complement(360..1922)
                     /locus_tag="AC16HB_13845"
     CDS             complement(360..1922)
                     /locus_tag="AC16HB_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HIR5607917.1"
                     /translation="MPPEPAQPTALVARRVGIDTYRENVAYLRRDCPVYRAEGFQALS
                     KVEVTPVDGGPRRSILAVVNVIDDTRLLGECELGLSEEAFADLGIEDGAPVAIAHAEL
                     PPSMQALKRKIGGERLARDEIGAIVRDIAHHRYSKIELAAFVVATNQFELDRDEVLYL
                     TEAMVDVGRRLDWAEKPVVDKHCIGGLPGNRTSMLVVPIVAAHGMLIPKTSSRAITSP
                     AGTADTMECLARVDLPFERMREIVREHRGCLAWGGRAALSPADDVLISVERPLSIDSP
                     GQMVASILSKKIAAGSTHLVLDIPLGPTSKVRHMDDAQRLKKLFEYVAHHVGMALDVV
                     ITDGRQPIGRGIGPALEARDVLQVLNNDPNAPIDLRNKALRLAGRVLEFDRDVRGGDG
                     FAVARDILDSGRALSKMNAIIDAQGRAAELPPLARLTHTVEAPTEGHVTAIDCETMAR
                     IARLAGAPKVKGAGVDLLKKLGDKVERGEPLYRTHADTATELAFARDAAARANGFTIG
                     LPAPIANGYVEF"
     gene            complement(1912..3309)
                     /locus_tag="AC16HB_13850"
     CDS             complement(1912..3309)
                     /locus_tag="AC16HB_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR5607918.1"
                     /translation="MRLTFLGAAGEVTGSCTLVESGSARFLIDCGMFQGASRGPLSAA
                     QKNRNAYQRDLGRIDFVVLTHAHVDHCGLLPRFARASRTQAQPPIYCTRPTADLVPVM
                     LKDSAHVQARGNRHGRRAPATEEPLYTMDDVDRLAMRLHSVPYDVEFQPHPEVRIRLR
                     NAGHIVGAASVQVHLTDGARTRTLLASGDLGEASPLLVSDRAPPPEAEVIVVESTYGD
                     RNHRSLADTEAELIEVLDDVLHQRHGNVIVPAFALGRTQELLVLLFRLARAGRVRVPL
                     VFVDSPLAQQASDVTFAHLDALDELAAEFHAAQQARRLPFALRYTESVEDSMFLNSIR
                     QGAVIIAASGMCEAGRIRHHLRHNLSRRECAVLFTGFQAAGTLGRRLVDGVASVSLFG
                     ESIPVRASIHTLGGLSAHAGQDSLMDWLRAARKPKSAFVTHGEPEAAHALSARIAREL
                     RWSPTIAEAGKTYAA"
     gene            3390..4925
                     /locus_tag="AC16HB_13855"
     CDS             3390..4925
                     /locus_tag="AC16HB_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5607919.1"
                     /translation="MPDLVLTTLNARYAHASLALRYLRANLGDLRDGSVIVEFVLGRR
                     AEDVVEQLLSFSPRVIGFGVYIWNVEETTRIVALLKTLAPDVAVVVGGPEVSHEVDAQ
                     RICALADHVVTGWGDVTFARLARAILRGPRPLSNVHAGEQPPLDGIAAPYREFSDADL
                     RRRHVYVEASRGCPFKCEFCLSALDRTAWPFPLAPFLDELARLYERGARHFKFVDRTF
                     NLKIESSIAILRFFLERIEAHPEDPPFVHFELIPDHLPQRLREWIARFPPGVLQFEIG
                     IQTWNEDVQKLISRRQDNTAAEDNLRWLRAHTHAHLHVDLIAGLPGEDLASLARGFDR
                     LVALNPHEIQLGILKRLRGAPIARHTQSFGLRFNPDPPYNVLATDRIDFASLQRVARF
                     ASYWELVANSGRFARTLQCVLGDAPFERFMALADWLYAQTGRTHAIANEELYELLYRR
                     LSEVNDVAPARAAAALAADYIGSGARGRLSFADTAHAARRERETAAAPRRQRRHLRAA
                     TSS"
     gene            complement(4912..>5118)
                     /locus_tag="AC16HB_13860"
     CDS             complement(4912..>5118)
                     /locus_tag="AC16HB_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5607920.1"
                     /translation="RRARKTMGTIVDDLMTADPVTIRETDTLERAVELMTRHRIKRLV
                     VADDARQVRGVVSRGDLVRLFTMK"
BASE COUNT          740 a         1792 c         1773 g          813 t
ORIGIN      
        1 gatcacggtg tcgaaaagcg atgccaggaa tttcccgacg atgcgctggc tgaccgcctc
       61 gccagggctg aaagcgatgt cctgccgcat gtagccgagg tacggcgcga ccagcaccag
      121 ttcgcgcgcg ccaagttcgc gcgcggtccg tgcaacgagc atcagctcga tgagcttctc
      181 gttcggctgg tgcagcgagc gcagcgcgac cacgcgcggc ggcagcgcct ggggcagcgt
      241 gagcctcagt tcgccgtcgg gaaagcggtg gcgcgcgatc cgctgcggcg cgagcccggt
      301 tgcagccgcg agttgctgcg caaaggtcgc ctcgtcctcg aagtgcagca gcatcgaccc
      361 tagaactcca cgtagccgtt cgcgatcggc gccggcagcc cgatcgtgaa tccgttcgcg
      421 cgcgccgcgg cgtcgcgcgc aaaagcgagt tcggtcgcgg tgtcggcgtg ggtccggtac
      481 agcggctcgc cacgttcgac cttgtcgccg agcttcttca gcagatccac gcctgccccc
      541 ttcaccttcg gcgcgccggc cagccgcgcg atgcgcgcca ttgtctcgca atcgatcgcc
      601 gtcacgtggc cttcggtcgg cgcttcgacc gtgtgcgtca gccgcgccag cggcggcagt
      661 tcggcggcgc ggccctgggc gtcgatgatc gcgttcatct tggacagcgc gcggccggag
      721 tcgaggatgt cgcgcgcaac cgcgaagccg tcgccgccgc gcacgtcgcg atcaaactcc
      781 agcacgcggc ccgccaagcg cagcgccttg ttccgcagat cgatcggggc gttggggtcg
      841 ttgttcagca cctgcagcac gtcgcgcgcc tccagcgccg ggccgatgcc gcggccgatc
      901 ggctggcgcc cgtcggtgat caccacgtcg agcgccatgc cgacgtggtg ggcgacgtac
      961 tcgaacagct tcttcagccg ctgtgcgtcg tccatatgcc gcaccttgga cgtcggcccg
     1021 agcgggatgt cgagcacgag gtgcgtcgag ccggccgcga tcttcttgct caggatcgac
     1081 gcgaccatct gtcccggcga atcgatcgac agcggccgct cgaccgagat cagcacgtcg
     1141 tcggccggcg acagtgcggc acggccgccc cacgcgaggc agccgcgatg ctcgcgcacg
     1201 atctcgcgca tgcgttcgaa cggcaggtcg acgcgcgcca ggcattccat cgtgtcggcg
     1261 gtgccggcgg gcgaggtgat cgcgcggctc gatgtcttgg ggatcagcat gccgtgcgcg
     1321 gcgacgatcg gcaccaccag catcgaggtg cgattgccgg gcagcccgcc gatgcagtgc
     1381 ttgtccacca cgggcttctc ggcccagtcc agccggcggc cgacgtcgac catcgcctcg
     1441 gtcaggtaca gcacctcgtc gcgatcgagc tcgaactggt tggtcgcgac cacgaacgcg
     1501 gccagctcga tcttcgagta gcgatggtgc gcaatgtcgc gcacgatcgc gccgatctcg
     1561 tcgcgcgcca gccgttcgcc gccgatcttg cgcttcagcg cctgcatcga cggcggcagc
     1621 tcggcgtgcg cgatcgcgac cggcgcgcca tcctcgatgc cgaggtcggc aaacgcctct
     1681 tccgacaggc cgagttcgca ctcgcccagc agccgcgtgt cgtcgatcac gttgacgacc
     1741 gcgaggatcg atcgccgcgg cccgccgtcg acgggcgtga cctcgacctt cgaaagcgcc
     1801 tggaagccct cggcgcggta gacgggacag tcgcgtcgca ggtacgcgac gttctcgcgg
     1861 taggtatcga tgccgacgcg gcgtgccacg agggcagtcg gctgcgcggg ctcaggcggc
     1921 ataggtcttc cccgcttcgg cgatggtcgg gctccagcgc agttccctgg cgatgcgtgc
     1981 gctcagggcg tgcgcggctt ccggctcgcc gtgcgtcacg aacgcggact tcggtttgcg
     2041 ggccgcgcgc agccagtcca tcagcgaatc ctgccccgcg tgtgcggaca atccgccgag
     2101 cgtgtggatc gacgcgcgca ccggaatcga ttcgccgaac agcgacacgc ttgccacgcc
     2161 gtcgacgagc cgccggccga gcgtgccggc ggcctggaag ccggtgaaca gcacggcgca
     2221 ttcgcggcgc gacaggttgt gccgcaggtg atggcggatg cgccccgcct cgcacatgcc
     2281 gctggctgcg atgatgaccg cgccctggcg gatcgagttc aggaacatcg aatcctcgac
     2341 gctttcggtg tagcgcagcg cgaacggcag cctgcgggcc tgctgcgcgg cgtgaaactc
     2401 ggccgccagc tcgtcgagcg cgtcgaggtg cgcgaaggtc acgtcgctcg cctgctgcgc
     2461 cagcggcgag tcgacgaaaa ccagcggcac gcgcacgcgg ccggcgcgcg ccagccggaa
     2521 cagcagcacc agcagttcct gcgtgcgacc gagcgcgaac gccggcacga tcacgttgcc
     2581 gtgacgctga tgcagcacgt cgtcgagtac ctcgatcagc tcggcctcgg tgtcggcgag
     2641 cgagcggtgg ttgcgatcgc cgtaggtgga ttcgacgacg atcacctcgg cctccggggg
     2701 cggcgcacgg tcgctgacca gcagcggcga tgcctcgccg aggtcgcccg acgcgagcag
     2761 cgtacgcgtg cgggcgccat cggtgaggtg aacctgcacc gatgcggcgc cgacgatgtg
     2821 cccggcgttg cgcaggcgga tccgcacttc ggggtgcggc tggaactcga cgtcgtacgg
     2881 cacgctgtgc aaccgcatcg cgaggcggtc gacgtcgtcc atcgtgtaca gcggttcttc
     2941 ggtggccggc gcgcggcggc cgtgacggtt gccgcgcgcc tgcacgtgcg cgctgtcctt
     3001 cagcatcacc ggcacgaggt cggcggtcgg ccgcgtgcag tagatcggcg gttgcgcctg
     3061 cgtgcgcgac gcgcgcgcga agcgcggcag cagcccgcaa tggtcgacgt gcgcgtgcgt
     3121 gagcacgacg aaatcgatgc ggccgaggtc gcgctggtac gcgttgcggt tcttctgcgc
     3181 ggcggagagc ggcccgcggc tcgcgccctg gaacatgccg cagtcgatca ggaagcgcgc
     3241 cgatccggac tcgaccagcg tgcacgagcc ggtcacctcg ccggcggcgc cgagaaatgt
     3301 cagccgcatg ggcccgcatc catttccatc gcagccagtc tacgcgggac cctcgggaga
     3361 ctgtccttgc gcgcggtcat aatcgctgcg tgccggacct cgtcctgacc acgctgaacg
     3421 cccgctacgc gcacgcgtcg ctcgcgctgc gttatctgcg tgcgaacctc ggcgatctgc
     3481 gcgacggcag cgtgatcgtc gagttcgtgc tgggtcgtcg cgcggaggac gtggtcgagc
     3541 agcttctttc gttttcgccg cgcgtgatcg gcttcggcgt gtacatctgg aacgtcgagg
     3601 agacgacgcg catcgtcgcg ctgctgaaga cgctcgcgcc ggacgtggcg gtcgtcgtcg
     3661 gcgggccgga ggtcagccac gaggtcgacg cgcagcgcat ctgcgcactc gcggaccacg
     3721 tcgtcaccgg ttggggcgac gtgacgttcg cccggctggc ccgcgcgatc ctgcgcggac
     3781 cgcgtccgct ctccaacgtg cacgcgggcg agcagccgcc gctcgatggg atcgcggctc
     3841 cgtaccgtga gttcagcgac gccgatctgc gccggcgtca cgtgtacgtg gaagcatcgc
     3901 gcggctgccc attcaagtgc gaattctgcc tgtcggcgct cgaccggacc gcatggccgt
     3961 ttccgctcgc gccgttcctc gacgagctgg cacgtctgta cgagcgcggc gcgcggcact
     4021 tcaagttcgt cgaccgcacc ttcaacctga agatcgagtc gtcgatcgcg atcctgcgct
     4081 tctttctcga gcgcatcgag gcacatccgg aggatccgcc attcgttcac ttcgaactga
     4141 ttcccgacca cctgccgcag cggctgcgcg agtggatcgc gcgctttccg cccggcgtgc
     4201 tgcagttcga gatcggcatc cagacctgga acgaggacgt gcagaagctg atctcgcgcc
     4261 gacaggacaa cacggcggcg gaggacaacc tgcgctggct gcgcgcgcac acgcacgcgc
     4321 acctgcacgt ggatctcatt gccgggctgc cgggcgagga cctggccagc ctcgcgcgcg
     4381 gtttcgaccg gctcgtggcg ttgaatccgc acgagatcca gctcgggatt ctcaagcggc
     4441 tgcgcggcgc gccgatcgcg cgccacacgc agtcgttcgg cctgcgcttc aatcccgatc
     4501 cgccgtacaa cgtgctggcg accgaccgca tcgactttgc gtcactgcag cgggtcgcgc
     4561 gcttcgcgag ctactgggag ctcgtcgcca actcgggccg ctttgcgcgc acgctgcaat
     4621 gcgttctcgg tgatgcaccg ttcgagcgct tcatggcgct cgcggactgg ctgtacgcac
     4681 agaccggccg cacgcacgcg atcgccaacg aggaactgta cgagctgctg tatcgccggc
     4741 tgagcgaggt gaatgacgta gcgccggcgc gcgcggccgc ggctctcgcc gccgattaca
     4801 tcggcagtgg cgcgcgcggc cgcctgtcgt tcgccgatac cgcgcacgcg gcgcgcagag
     4861 aacgcgagac cgccgcggcg ccgcgccggc agcggcgcca cctgcgcgcc gctacttcat
     4921 cgtgaagagc ctgaccaggt cgccgcgcga cacgaccccg cgcacctgac gcgcgtcgtc
     4981 cgcgaccacg agccgcttga tccggtggcg cgtcatcagc tcgacggcac gctccagcgt
     5041 gtcggtttcc cggatcgtca ccgggtcggc agtcatcaga tcgtcgacga tcgtgcccat
     5101 cgtcttgcgc gcgcggcg
//
LOCUS       DBNHBY010000569         3564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_37597, whole genome shotgun sequence.
ACCESSION   DBNHBY010000569 DBNHBY010000000
VERSION     DBNHBY010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_37597"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="AC16HB_13865"
     CDS             <1..1367
                     /locus_tag="AC16HB_13865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5607921.1"
                     /translation="DHFGLDPLIVAAVKEMGYTTPTPIQSKAIPVVLKGRDVMGAAQT
                     GTGKTAGFALPIIQRLLSQANPSMSPARHPVRSLVLAPTRELAEQVAENFKKYISKTA
                     LRCATVYGGVDMDPQTQALRGGVEIVVATPGRLLDHLQQKNTSLSQVQIVVLDEADRM
                     LDMGFLPDISRILNLLPKERQSMMFSATFSEEIKKLAANFLRDPLLLEVARRNAPAEL
                     IKQEVVRVHESEKTDALLTLLRTRGADGGRMTQVLVFVNAKIECRRLARQLQKAGVNA
                     DAIHGDKSQDERMKALDAFKKGEVEVLVATDVAARGLDIVELPAVINYDVPFNPEDYV
                     HRIGRTGRAGASGLAIMLMTGADERGVQAIEKLTKQKFEALAIAVERGRGERRDRPPR
                     RERGVGSARPNAAPQVVDDFFSKPYEPATDESDERVEEDAAAPRERGKKAVRVAALLG
                     GSKR"
     gene            complement(1390..2229)
                     /locus_tag="AC16HB_13870"
     CDS             complement(1390..2229)
                     /locus_tag="AC16HB_13870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5607922.1"
                     /translation="MITTRPHVRSDGDGPDVVLLHSSGSSGRQFDALVERLASRFRLH
                     TVDLHGHGSTPAWSEARAMTLADDAALVEPLLAACPRGVHVVGHSYGGAVALNLALRH
                     PQRVLSVTAYEPVVFRVLFEYGLRQAEAQSVLATARAMRARLAHGDNERAAQRFVDFW
                     SGAGAWNAMPLARRQAVAARMPSVMRHFDALFCDTLQRTQISTLAMPALFLTGERTVA
                     ATRRIGELLRHSARRARHEMLPGLGHLGPVTHAPMVIERVADFLGTVAVRRARLEPLR
                     AAA"
     gene            complement(2283..3239)
                     /locus_tag="AC16HB_13875"
     CDS             complement(2283..3239)
                     /locus_tag="AC16HB_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607923.1"
                     /translation="MEAVAEVANPQSASRYAKCVEVSKRVRWDIDRDVLRGRQFDFGK
                     KFLPDALARQGALTFLTPSDHRFLSQIQGRTYANTFGLVERFIGPKIAEVSRDHWLGD
                     QKAFEALVRFTDEELKHQELFRRLDAMAAEGMPAGYTYVPQPNEVAGFVLGKSTWAVL
                     ALTCHIEIFVLSHYHQSIDPDSDLSPLWKDVFLYHAREESQHAILDELEWAREDARIT
                     PEQRDRAVDELIELVAAVDGILVAQGAADANYFAATCARALTAEQIRAVEATLLAAYR
                     WQYIVSGVQDERFQQALHAKITPAQFARIGNALGPILASTAQ"
     gene            complement(3280..>3564)
                     /locus_tag="AC16HB_13880"
     CDS             complement(3280..>3564)
                     /locus_tag="AC16HB_13880"
                     /inference="COORDINATES: protein motif:HMM:NF018294.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HIR5607924.1"
                     /translation="LRAGDAESDRIARSHPAYRLSELIVELGASLADTVRRAWQRWQR
                     WRDAQAGRRALHELDTRTLHDIGVTRSDTWTAAADASRMRAPLPLRGPAL"
BASE COUNT          585 a         1204 c         1240 g          535 t
ORIGIN      
        1 tcgaccattt cggccttgat ccgctgatcg tcgcggcggt caaggagatg ggctacacga
       61 cgcccacgcc gatccagagc aaggcgattc ccgtcgtgct caagggacgc gacgtgatgg
      121 gcgccgcgca gacgggcacc ggcaaaaccg cgggctttgc gctgccgatc atccagcggc
      181 tgctgtcgca ggccaacccc agcatgtcgc cggcgcgtca cccggtacgc tcgttggtgc
      241 tggcgccgac gcgagagctc gcggagcagg tcgccgagaa cttcaagaaa tacatcagca
      301 agaccgcgct gcgctgcgcg accgtctacg gcggcgtcga catggacccg cagacgcagg
      361 cactgcgcgg cggcgtcgag atcgttgtgg caacgcccgg ccggctgctc gaccacctgc
      421 agcagaagaa cacgtcgctg tcgcaggtgc agatcgtcgt actcgacgag gccgaccgga
      481 tgctcgacat gggctttttg cccgacatct cgcgcattct gaatctgctg ccgaaggagc
      541 ggcagagcat gatgttctcg gcgacgtttt ccgaggagat caagaagctc gccgccaatt
      601 tcctgcgcga tccgctgctg ctcgaagtcg cgcgccgcaa tgcacccgcc gagctgatca
      661 agcaggaagt cgtgcgtgtg cacgagtccg aaaaaaccga cgcattgctc acgctgctgc
      721 gcacgcgcgg cgccgacggc ggccgcatga cgcaggtcct ggtgttcgtc aacgccaaga
      781 tcgagtgccg gcgcctggcg cggcagttgc agaaggcggg cgtgaacgcg gacgcgatcc
      841 atggggacaa gtcgcaggac gagcggatga aagcgctcga cgcgttcaag aaaggcgagg
      901 tcgaggtgct cgtcgccacc gacgtggcgg cgcgtgggct cgatatcgtg gaactcccgg
      961 cagtgatcaa ctacgacgtg ccgttcaacc ccgaggacta cgtgcaccgg atcggccgta
     1021 ccggtcgcgc cggcgcctcg ggcctggcga tcatgctgat gaccggcgcc gacgagcgcg
     1081 gcgtgcaggc gatcgagaag ctcaccaagc agaagttcga ggcgctcgcg atcgcggtcg
     1141 aacgcgggcg cggcgagcgg cgcgatcgtc cgccgcggcg cgagcgaggc gtcgggtcgg
     1201 cacgcccgaa cgcggcgccg caggtggtcg atgacttctt ctccaagccg tacgagcccg
     1261 cgacggacga gtccgacgag cgggtcgagg aagatgccgc ggcgccccgc gagcggggca
     1321 agaaggccgt gcgcgtcgcc gcgctgctcg gcggcagcaa gcgctgatca cagttcagcg
     1381 tttcccggct caggcagccg cgcgcagcgg ttcaagcctc gcgcgacgga ctgccacggt
     1441 gccgaggaag tccgcgacgc gctcgatgac catcggcgca tgcgtgaccg gaccgagatg
     1501 acccagtcca ggcagcatct cgtgccgcgc gcgccgcgct gaatgacgca gcagttcccc
     1561 gatccgccgc gtggccgcga ccgtgcgctc gccggtcagg aagagtgccg gcattgccag
     1621 cgtcgagatt tgcgtgcgct gcagggtgtc gcagaacagc gcgtcgaagt gcctcatgac
     1681 cgacggcatg cgggccgcca ccgcctgccg gcgcgcgagt ggcatcgcat tccacgctcc
     1741 ggcgccggac cagaagtcga cgaagcgctg agcggcgcgt tcgttgtcgc cgtgcgccag
     1801 ccgcgcgcgc atcgcccggg cggtcgcgag cacgctctgc gcctctgcct gacgcaggcc
     1861 gtactcgaac agcacgcgaa acacgaccgg ctcgtacgcc gtcacgctca gcacgcgctg
     1921 cgggtgcctc agcgccagat tcagggcgac cgctccgccg tacgaatggc cgacaacatg
     1981 cacgccgcgc gggcacgccg cgagcagcgg ctcgaccagc gcggcgtcgt cggcaagcgt
     2041 catcgcgcgc gcttcgctcc acgccggcgt gctgccgtga ccgtgcaagt ccaccgtgtg
     2101 caggcggaag cgcgacgcca ggcgctcgac cagcgcatcg aactggcgcc cggagctgcc
     2161 actcgaatgc agcagcacga cgtccggtcc gtcgccgtcg ctgcgaacat gaggtctggt
     2221 ggtgatcatc gtcgactcgg cacggtggcg cgcggctgcg gaggccgccg cgcgctatgc
     2281 cgtcactgcg cggtcgacgc caggatcggt cccagcgcgt taccgatgcg ggcgaactgc
     2341 gccggagtga tcttcgcgtg caacgcctgc tggaagcgct cgtcttgcac gccgctcacg
     2401 atgtactgcc agcggtacgc ggcgagcagc gttgcctcga ccgcccggat ttgctccgcg
     2461 gtcagcgcac gcgcgcaggt cgcggcgaag tagttggcgt cggcggcgcc ctgcgcgacc
     2521 aggatgccgt cgaccgcagc gaccagctcg atcagttcgt cgaccgcgcg atcgcgctgc
     2581 tcgggcgtga tcctggcgtc ctcgcgcgcc cactccagtt cgtcgaggat tgcgtgctgc
     2641 gattcctccc gcgcgtgata cagaaacacg tccttccaca gcggcgacag atcggaatcc
     2701 ggatcgatgc tctggtggta gtgcgacagc acgaagattt cgatgtggca ggtcagcgcg
     2761 agcaccgccc aggtcgactt gccgaggacg aagccagcga cctcgttggg ctgcggcacg
     2821 tacgtatagc cggcgggcat gccttccgcg gccatcgcgt cgaggcggcg gaacaactcc
     2881 tggtgcttca gttcctcgtc ggtgaaacgc accagcgctt cgaatgcctt ctggtcgccc
     2941 agccagtgat cgcgactcac ctcggcgatc ttcggcccga tgaagcgctc gaccagcccg
     3001 aaagtgttcg cgtacgtgcg accctggatc tgactcagga accggtggtc ggacggcgtg
     3061 aggaacgtca gcgcgccctg gcgcgccagc gcgtccggca ggaacttctt gccgaaatcg
     3121 aactgacgtc cgcgcagcac gtcgcggtcg atgtcccagc gcacgcgctt ggacacttcg
     3181 acgcacttcg catagcggct tgcggactgc gggttggcta cttcagcgac tgcttccatg
     3241 tgcatcctcc ggtgggagtg ggtgggcgaa aggcgaggtt cagagcgcag gcccgcgcag
     3301 tggcagcggc gcgcgcatgc gggatgcgtc cgctgcggcg gtccacgtgt cgctgcgcgt
     3361 cacgccgatg tcgtgcagcg tgcgggtgtc gagttcgtgc agcgcacgcc ggcctgcttg
     3421 ggcgtcacgc cagcgctgcc agcgctgcca ggcgcgccgc acggtgtcgg ccagcgacgc
     3481 gccgagttcg acgatcagtt cggacagccg ataggcgggg tgcgagcgcg cgatgcgatc
     3541 ggactccgcg tctccggcac gcag
//
LOCUS       DBNHBY010000570         9495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_783205, whole genome shotgun sequence.
ACCESSION   DBNHBY010000570 DBNHBY010000000
VERSION     DBNHBY010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_783205"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1441)
                     /locus_tag="AC16HB_13885"
     CDS             complement(80..1441)
                     /locus_tag="AC16HB_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR5607925.1"
                     /translation="MQSMKVICPNGHLGFAPLKPGSFRLGVEAGPDIIAADSGSDDVG
                     PVPLGSDTSTSPLAWQTQDLEAMLLASRELKVPMIIGSAGDTGANSRVDLYLKIIHEL
                     AAKHRLPRFKLGWFYSDVDREWLRAKIRSGDTVTGLDARAALDEAELDATDRIVAMAG
                     VHPYIKLLDLGADVVIGGRSSDCAVFAAPAIRAGFPEALSYYLGKVLECASFCAEPYG
                     GKETVLGEISRDDVKVTAMHPDQRCTVASVAGHAMYERSSPYFEYVAGGTLDMRECRY
                     EQVGPRTTRITGPRFIPIDRVRVKLEGSGKVGERFVGIAGVRDPYTIANIDRVIGWAR
                     DQVAERFGTSGYELHYTVYGRDGIMGAMEPMRDRPGHELCVVVQGVAPTREMAEEVCM
                     TGTRQMFYARLPDVKGTAGSVAFVLDEVMPASPAYRWTMNHTVGIDDPLELFPCHVAE
                     VGG"
     gene            complement(1463..2527)
                     /locus_tag="AC16HB_13890"
     CDS             complement(1463..2527)
                     /locus_tag="AC16HB_13890"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR5607926.1"
                     /translation="MNLEDGLPLKLAGSPGERGASQARTRPALVEAVRTATRQRLEEC
                     AAARRLPVVRDYLEHEKAFAREHCAPEMAELEGIAAGFGVQWEELFDSLHMSPITDLA
                     AALDACTAWAAVWNDGGAAVVKNRDVRAEFAPLQDVMLHEDPMQTMRTVLCVGSLGAP
                     GVYSSGINRAGLVVVDTAIRSRSYRPGWLRYFLMTRLLWTCATVAESLDFLRAVPHTG
                     GGSLVLGDETGAVAAVEFPDGQPAVESADMVSRTNHFTTAGAKLPATTNDRNEANSAA
                     RLDYLREVLEGRGIPRTTQAVAELMAAHGDGDQALCRHGEGREVNTISTVLYLTRARE
                     LIFAPDNPCRARWRRYSLTA"
     gene            complement(2524..3537)
                     /locus_tag="AC16HB_13895"
     CDS             complement(2524..3537)
                     /locus_tag="AC16HB_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607927.1"
                     /translation="MNDTAPPLAAGPLVDVRGLTKEFELARSVGARLLRAPRRVVRAV
                     NDASFSIARGETLGLIGESGCGKSTVARLLLRLHEPTRGQVMLNGIDITTLAGADLMR
                     FRRNMQIMFQDPYASRNPRKTVEQIVAAPLVLHERLSWREARERVADFIGRVGLQSSQ
                     LQRYPHQFSGGQRQRIGIARALILKPEFVVCDEPVSALDVSIQAQIIDLLQSLKSELG
                     LTYLFISHDLAVVGYLSDRIAVMYLGEIVETAPMRALLASPAHPYTQALLSAVPRLER
                     GRQRDRIRLTGDLPSPLAPPAGCKFHTRCPAAMEICRRQVPALTSIGDDRRVACHLYP
                     DAK"
     gene            complement(3542..4570)
                     /locus_tag="AC16HB_13900"
     CDS             complement(3542..4570)
                     /locus_tag="AC16HB_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5607928.1"
                     /translation="MHSTPASTTEGGRSAAAEAEALLSIRGLAVRFATRAGIVTAVDG
                     VDLDVFPGECLGLVGESGSGKSVTFAAVMGLIRPPGKVAEGHISFRGCDVLALGDAER
                     RALRGRDIAMTMQDALTALNPAFTVASQIIEVLLAHDRADSRAQARTQAIELMRLVGI
                     PAAERRLDDYPHQFSGGMRQRIMVAIALACRPKLLIADEPTTALDVTIQAQVLELIAS
                     LREKLGMSVVLITHNLGVVAEYCDRVAVMYAGQIVETGPTADVIESPRHPYTQGLLQS
                     IPRLDHLGERIRPIEGALPDLSRLGEICRFEPRCRLRTAACRTRIPMFDLGPQRLARC
                     IRVHDTDT"
     gene            complement(4542..5411)
                     /locus_tag="AC16HB_13905"
     CDS             complement(4542..5411)
                     /locus_tag="AC16HB_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607929.1"
                     /translation="MIAARSPTAESFARFLDNRLGVASAIVVIAIVLLAIGAPWIAPH
                     DPNDADLLRRLQPPVWLEGGEWSFALGCDALGRDILSRLMYGARVSIAVGAIVILFAT
                     AFGIATGLVAGYLRGRIDTLISRLVDILLGFPYLIFAIGLMAMLGPGLHNVAITLIAK
                     EWVVPCRLVRGETLAAREMEYVEAARALGVGRLGIMLHEILPNILSPVIVVATIRLAH
                     VIILEASLSFLGLGVQPPTASWGSMVADGREFMLDAWWVSTFAGLAILILVLAINVAS
                     QGLRDAFDPRFHD"
     gene            complement(5441..6442)
                     /locus_tag="AC16HB_13910"
     CDS             complement(5441..6442)
                     /locus_tag="AC16HB_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5607930.1"
                     /translation="MGTVVDRLLQLVPVLLGVAVIVFVMITLTPGDPVEIMLGDMKAA
                     PEQAEALRRDMGLDRPPVERFFHFIGNAVQGNFGLSFFHRRPVIDVIAERLPATIELT
                     LAALVLALAISIPLGALAAVHKGGALDRIATIGSLLGVSMPGFWFGILLIMLFSVQLG
                     VLPVSGRIGYGNEVPAVTGLLTIDTLLAGRFDSFVQALRHLALPSITLALPMAAPLMR
                     VTRASMLEVLRSDYVTFAEAKGLSRRQVLWRHALKNALMPTVTIAALETGSLLGGNMI
                     VESVFGWPGLGRLVVESIFARNYPLVQAAVLLYAVTYVLLNFLADVAYTLLNPKVRL"
     gene            complement(6462..7835)
                     /locus_tag="AC16HB_13915"
     CDS             complement(6462..7835)
                     /locus_tag="AC16HB_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607931.1"
                     /translation="MCDGLLTRDSHMKVVPEVAESYRQIDAKTWEFKLRKNVRFHSGA
                     VLTAEDVKFTLDRLTRENAMGGQTSPRKDLLGPISSVDVVDPQTVRILLSSPWPILPA
                     MLPFQELVRKGFVEKVGSAGLATEVDCVGPFKLVEWRHGDSIIMERFADYYGGSPEIP
                     PVGPAKVDRVIFRVIPENASRVAALLAGEVDIINELPVSAMKQVDASGRAKVMRANGT
                     RTFFVALNTAKKPFDDPRVRKALNHAIDRKLIIDRVLSGTAVPLNGVLSPQAFGFNAD
                     LPEYKYDPNRAKTLLAEAGYGSGLTLTLDTEGAFKDVAEAIASMLQRAGVKATVQVWE
                     GAVLTPIWRDPAKRKERDMYFTSWGNGSLDPSDIMLPTLRTGGRGNSAGYSNAKVDQL
                     LDAAETELDPAKRRALYVESQSIVNADAPWIFLWLPQDLYGVSTRVSGWVPSPDSRIN
                     LHRAMVR"
     gene            complement(8583..9014)
                     /locus_tag="AC16HB_13920"
     CDS             complement(8583..9014)
                     /locus_tag="AC16HB_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607932.1"
                     /translation="MKRVARKKVEADDTGAASILRGLFRSFAKLAEQAGDGRPLLRGL
                     RVAHDPKADRFVVVHSGSRVEFVLVLHGADQPPCAEVECRRMDSAGATEATPIARLRF
                     NDTGVVSQSTVAELVNERIDQAPGAWSIVAAVIWDALVSRG"
     gene            complement(9076..9324)
                     /locus_tag="AC16HB_13925"
     CDS             complement(9076..9324)
                     /locus_tag="AC16HB_13925"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HIR5607933.1"
                     /translation="MAREFTRANARELTVHHRDHDHDNNPSDGSIWEPLCLYRHDNEH
                     QRQLDANGTGNSSSRDRVSTDTPCADLKVRLAGKPPEK"
BASE COUNT         1706 a         3177 c         2973 g         1639 t
ORIGIN      
        1 tcgaacgtga tcttgtcggt gcccgcgttc ttgctgcgga tcgtcttcgc cagttcggag
       61 agttttcggg ttgccatcgt catcccccga cctcggccac gtgacacgga aagagctcaa
      121 gcggatcgtc gatgccgacg gtgtggttca tcgtccagcg atacgccgga ctggccggca
      181 tgacctcgtc aagcacgaag gccaccgagc cagccgtccc cttcacgtcg ggcaaacgcg
      241 catagaacat ctgtcgcgtg ccggtcatgc acacttcctc cgccatctcg cgcgtcgggg
      301 ccacgccctg gacaacgaca cacagttcgt gtccgggccg atcgcgcatg ggctccattg
      361 cgcccatgat gccgtcgcgc ccgtagaccg tgtagtgcag ctcgtagcca ctggtgccga
      421 agcgctcggc cacctgatca cgcgcccagc cgatcacgcg atcgatgttg gcgatggtgt
      481 acgggtcgcg cacgccggcg atgccgacga agcgctcgcc aaccttgcct gaaccttcga
      541 gcttcactcg cacgcgatcg atcgggatga atcgcggccc cgtgatgcgc gtcgtcctgg
      601 gaccaacttg ttcataccgg cactcgcgca tgtcgagcgt gccgccagct acgtattcga
      661 agtatggcga ggaccgctcg tacatcgcgt gtcctgccac agaggcaacc gtgcagcgct
      721 ggtccggatg catcgcggtc accttgacgt cgtcacggct gatctctccg agcaccgttt
      781 ccttgccgcc gtaaggttcc gcgcagaacg atgcgcactc gaggaccttg ccgaggtagt
      841 acgagagcgc ctcgggaaag ccggcgcgaa tcgcgggggc agcgaagacc gcgcaatcgg
      901 aactgcggcc accgatgacc acgtccgctc ccaggtcgag tagcttgata tacgggtgca
      961 cgccggccat ggcgacaatg cggtcggtgg catcgagctc cgcctcgtcc agcgcagcgc
     1021 gtgcgtcgag tccggtcacc gtgtcgccgc tgcgaatctt tgcgcgcagc cactcgcgat
     1081 cgacatcgga gtaaaaccag ccgagcttga aacgcggcag acgatgcttt gccgccagct
     1141 catggatgat cttcaggtac aagtcgacgc ggctgttggc gccggtgtcg ccggccgagc
     1201 cgatgatcat cgggactttg agttcgcgcg aggcgagcag catcgcttcc agatcctgtg
     1261 tttgccatgc aagcgggctg gttgacgtgt cggagccaag cggcaccggg ccgacatcgt
     1321 cgctgcccga gtcggcggcg atgatgtcgg gccccgcctc gacacccagc cggaagctgc
     1381 ccggcttcag cggtgcgaag cccagatggc cgttggggca gatcaccttc attgattgca
     1441 tcagacctcc ggatcgaccg cctcaggctg tcagtgaata gcgccgccat cttgcgcggc
     1501 atggattgtc cggcgcgaag atcaactcgc gcgcccgggt caggtaaaga acggttgaaa
     1561 tcgtgttcac ttcacgcccc tcgccatgac ggcatagcgc ctgatctccg tctccatgcg
     1621 cggccatgag ctcggctacc gcctgcgtgg tccgtggtat gccacgtccc tcaagaacct
     1681 cgcggaggta atcaaggcgt gcggcagagt ttgcttcgtt ccgatcgttc gttgtcgccg
     1741 gcaacttggc gcccgcggtc gtgaagtgat tggtgcgtga caccatatcc gcactttcga
     1801 cggccggttg gccgtcagga aattcgactg cagcgaccgc tcctgtttca tcccccagga
     1861 cgagactgcc gccgccggta tgcggtaccg cgcgaaggaa gtcgagcgat tcggcgacgg
     1921 tggcgcaggt ccacagcaag cgtgtcatca ggaaatagcg caaccagccc ggacgatagg
     1981 agcgcgaccg gattgccgtg tcgaccacca ccagaccggc gcgattgatg ccgctcgaat
     2041 acacacccgg tgcaccgagc gagccgacgc atagcaccgt gcgcatcgtt tgcatcggat
     2101 cctcatgcag catcacgtcc tgcagcggcg cgaactcggc acgcacgtca cggttcttga
     2161 ccaccgccgc gccgccgtcg ttccacacgg ccgcccatgc cgtacaggca tccaacgccg
     2221 cggcgagatc tgtgatcggg ctcatgtgca gcgaatcgaa tagttcctcc cactgcaccc
     2281 caaagcccgc cgcgatgcct tcgagctcgg ccatttcggg cgcgcagtgt tcgcgcgcga
     2341 acgctttctc gtgttccaga taatcgcgca cgaccggcaa ccgccgcgcg gccgcacact
     2401 cttcgagtcg ctgccgcgtg gccgtgcgaa ccgcttcgac gagcgcgggt cgcgtccgcg
     2461 cctgcgaagc gccgcgttcg cctggggagc cagccaactt caacggcagt ccgtcttcga
     2521 ggttcatttc gcgtccggat ataggtggca cgccaccctc cggtcatcgc cgattgacgt
     2581 tagcgccggt acctgccgcc ggcagatttc catcgccgcc gggcagcgcg tgtggaactt
     2641 gcagccggcg ggcggtgcga gcggcgacgg cagatcgccg gtcagtcgga tccggtcgcg
     2701 ctgccggcca cgctcgagcc gcggcaccgc ggacagcagc gcctgtgtgt acggatgcgc
     2761 gggcgatgca agcagcgcgc gcatcggcgc ggtctctacg atctcgccca gatacatcac
     2821 ggcgatgcga tcgctcaggt agcccaccac cgcgaggtcg tgcgagatga aaagatacgt
     2881 caggccgagc tctgacttca gcgactgcag cagatcgatg atctgtgcct ggatcgacac
     2941 atcgagcgca gagaccggtt catcgcaaac aacgaattcc ggtttcagga tcagcgcgcg
     3001 ggcaatgccg atccgctgcc gctgcccgcc cgagaactgg tgggggtagc gctgcaactg
     3061 tgatgactga agtccgaccc ggccgatgaa atcggccacg cgctcgcgcg cttcgcgcca
     3121 cgacagccgc tcatgcaaca ccagcggcgc cgcgacgatt tgctcgaccg tcttgcgcgg
     3181 gttccgcgac gcgtagggat cctgaaacat tatctgcatg ttgcgccgaa aacgcatcag
     3241 gtcggcgcct gcgagggtgg tgatgtcgat gccattcagc atcacctggc cgcgtgtcgg
     3301 ttcgtgcagc cgcagcagca gtcgcgcaac cgtgctcttg ccgcagccgc tttcgccgat
     3361 cagcccgagc gtttcgccgc gcgcaataga aaagctcgca tcgttaaccg cacgtaccac
     3421 gcgtcgcggc gcgcgcaaga gacgtgcgcc gacgctgcgc gcgagttcga actccttggt
     3481 cagtccgcgc acgtcgacga gcggcccggc agccaatggg ggagcagtgt cgttcatcgc
     3541 gttatgtgtc cgtgtcgtgc acgcggatgc agcgcgccag gcgttgcgga ccgaggtcga
     3601 acatcggaat gcgcgtgcgg catgcggccg tacgcaacct gcagcgcggt tcgaagcggc
     3661 agatctcgcc gagtcgcgaa agatcgggca acgccccctc gatgggccgg attcgctcgc
     3721 cgaggtgatc cagccgcggg atcgactgca gcaagccttg cgtataggga tgccgtggtg
     3781 actcgatcac gtcggctgtc ggtccggttt ccacgatctg acccgcgtac atcactgcca
     3841 ctcggtcgca atactcggcc accacaccga ggttgtgcgt gatcagcacc acgctcatgc
     3901 cgagcttttc gcgcaggctc gcgatcaact cgagcacctg cgcctggatt gtgacgtcga
     3961 gtgcggtggt tggctcgtcc gcgatcagca acttcggccg gcaagccagt gcaatcgcca
     4021 ccatgatccg ttgacgcatg ccgcccgaaa actgatgcgg atagtcgtcg aggcgccgct
     4081 ctgccgccgg aatgccgacc agccgcatca gttcgatcgc ctgggtacgc gcctgtgcgc
     4141 gggagtctgc acggtcatgc gcaagcagca cttcgatgat ctggctggcg acggtgaacg
     4201 ccgggttcag cgccgtcagt gcatcctgca ttgtcatcgc gatatcgcgg ccgcgcagcg
     4261 cccgacgttc ggcatcgccg agcgccagaa catcgcaacc gcgaaagctg atgtgccctt
     4321 cggcaacctt gcccggtggc ctgataaggc ccatcacggc agcgaatgtg acgctcttgc
     4381 cggaacccga ctccccgacc agtccaaggc attcgccggg gaagacgtcg aggtcgacgc
     4441 cgtcgacggc ggtcacgatt cccgcgcgcg tcgcaaagcg caccgcgagg ccgcggatcg
     4501 acaacagcgc ttccgcctcg gccgctgccg agcgcccgcc ttcagtcgtg gaagcggggg
     4561 tcgaatgcat cgcgcagccc ctggctcgcc acgttgatcg ccagcacaag gatcagaatc
     4621 gccaggcccg caaaagtgct gacccaccag gcatccagca tgaactcacg cccatcggca
     4681 accattgagc cccacgaggc ggtgggcggc tgcacgccga ggccgaggaa cgacaggctg
     4741 gcttcgagga tgatcacgtg cgccaggcgg atcgtcgcca ccacgatgac cggcgagagg
     4801 atgttgggca gtatctcgtg cagcatgatg ccgagacggc cgacgccgag cgcgcgcgcg
     4861 gcttccacgt attccatctc gcgcgccgcc agcgtctcgc cgcgcaccag ccggcacggc
     4921 acgacccact ccttggcgat aagcgtaatc gccacgttgt gcagtcctgg accaagcatc
     4981 gccatcagcc caatagcgaa gatcaggtac ggaaacccga gcaggatgtc gaccaggcgc
     5041 gagatcagcg tatcgatgcg tccgcgaaga taccccgcaa cgagtccggt tgcgatgccg
     5101 aacgcggtgg cgaacagaat cacgatcgcg ccgacggcaa tggaaacgcg tgcgccgtac
     5161 atcaatcgcg acaggatgtc gcggccgagc gcgtcgcaac ccagcgcaaa gctccactcg
     5221 ccaccctcga gccacactgg tggctgcagc cggcgcagca ggtcggcgtc attgggatcg
     5281 tgcggcgcga tccacggcgc accgatcgca agcagcacaa tcgcaatcac gacaatcgca
     5341 gaggcgactc ccagccggtt gtccaaaaag cgcgcgaagc tttcggcggt cggcgaacgc
     5401 gccgcgatca ttggtgcaag cacgtctgca gcgttactgc tcacaatcgg accttcggat
     5461 tgagcagcgt gtaggccacg tcagcaagaa agttcaacag cacataggtc accgcgtaca
     5521 gcagcacagc ggcctgcacc agcggatagt tgcgcgcaaa gatcgactcg acgaccagtc
     5581 gcccgaggcc gggccagcca aacaccgact cgacaatcat gttgccgccg agcagcgacc
     5641 cggtttccag cgccgcgatg gtcacggtcg gcatcagcgc attcttcagc gcgtggcgcc
     5701 acagcacttg ccggcgcgac agacccttcg cttcggcaaa cgtcacgtag tcggagcgga
     5761 gcacctcgag catcgaggcg cgggtgacgc gcatcagcgg ggcggccatc ggcagcgcca
     5821 gcgtgatcga cggcaatgcg aggtgccgca acgcctgcac gaagctgtcg aagcggcccg
     5881 ccagtagcgt gtcgatcgtc agcagcccgg tcaccgcagg gacttcgttg ccgtagccga
     5941 tgcggcccga taccggcaac acccccagct gtactgagaa cagcatgatc agcaggatgc
     6001 cgaaccagaa acccggcatc gataccccaa gcagagagcc gatcgtcgcg atccgatcca
     6061 gcgcgccgcc cttgtggacc gctgccagcg cgccgagagg aatcgagatc gccagcgcca
     6121 gcacgagggc agccagcgtc aactcgatcg tggccggcag gcgttcggcg atcacatcga
     6181 ttacaggccg acggtgaaag aacgacagcc cgaaattgcc ctggaccgca ttgccgatga
     6241 agtggaagaa gcgttccacc ggcgggcggt cgagtcccat gtcccttcgc agtgcttcgg
     6301 cctgctccgg tgccgccttc atgtcgccga gcatgatctc caccgggtcg cccggcgtca
     6361 gcgtgatcat cacaaagacg atgaccgcga caccgagcag taccgggacg agttgcagca
     6421 gccggtcgac gaccgtgccc acgacgactt ccggaccgca gctacctgac catcgcgcgg
     6481 tgcagattga tacggctgtc cggcgagggc acccagccgc tcacgcgagt tgaaacccca
     6541 tagagatcct gaggcagcca taggaagatc catggcgcgt ccgcgttgac gatcgactgt
     6601 gattcgacgt acagcgcacg gcgcttggct ggatcgagtt ccgtctccgc cgcgtcaagc
     6661 agctggtcga cctttgcgtt gctgtagccc gcactgttgc cgcggccacc agtgcgaagc
     6721 gtcggcagca tgatgtccga cggatcgagc gagccgttgc cccacgacgt gaagtacata
     6781 tcgcgctcct tgcgcttggc cgggtcgcgc cagatcggcg ttagcactgc gccctcccac
     6841 acctgcaccg tcgccttgac gcccgcccgc tgcagcatgc tcgcgatggc ctcggcgacg
     6901 tccttgaatg cgccttcggt gtcgagcgtc agcgtcagac cgctgccgta gccagcctcg
     6961 gcgagcaggg tcttggcgcg attcgggtcg tatttatatt ccggcaggtc ggcgttgaag
     7021 ccgaacgcct gcggcgacaa cacgccgttc aacggcactg cagtgccgct caggacccgg
     7081 tcgatgatca gcttgcggtc gatggcgtga ttgagcgcct tccttacgcg cggatcgtcg
     7141 aacggcttct tcgccgtatt caacgcgaca aagaacgtgc gcgtgccatt ggcgcgcatc
     7201 accttggcgc gtccgctagc gtcgacctgc ttcatcgcgg acaccggcag ttcgttgatg
     7261 atgtcgacct caccggccag caatgcggca acacgcgatg cgttctcggg aatgacgcgg
     7321 aagatcacgc gatcgacctt ggcgggaccg acaggcggga tctcgggcga accaccgtag
     7381 tagtcggcga agcgctccat gatgatcgag tcgccgtggc gccactccac gagcttgaac
     7441 ggtccgacgc agtcgacctc cgtggcgagg ccggcactgc cgaccttctc gacgaatccc
     7501 ttgcggacga gttcctggaa cggcagcatc gccggcagga tcggccacgg gctcgacagc
     7561 aggatgcgca ccgtttgcgg gtccacgacg tcgacgctgg agatcggtcc gagcaggtcc
     7621 ttgcgcgggc tggtctgccc gcccatcgcg ttttcccggg tcaaccggtc gagcgtgaac
     7681 ttcacgtcct cggcggtgag caccgcgccg ctgtgaaagc gcacgttctt gcgcaacttg
     7741 aactcccagg tctttgcgtc gatctgccga taggactcag caacctccgg cacgaccttc
     7801 atgtggctgt cgcgcgtcag cagcccatcg cacatgttgc ggatgatcgt ttccgtttct
     7861 cgttttctgt ggttcgcggg gtcaagcgtt agcgcatctt gggaaaagcc gactcgcagg
     7921 tccaccgcgc cggcagagac agtcagcgcg gccagaacac atcccagagc gaacaattgc
     7981 cgatacatgt tgtctccgca gaagtgaagc gcgcaatagc cggttcaacc agcgcgcatc
     8041 gaacacgcga tcgtgtgtct gtgcgcaacc gtagaacagc agcacgcatg cgtcaacgcc
     8101 agatattcgc caatgtggcc cgcgcgtctg gacatcacat tgtgattgcc ttggacgttg
     8161 cccgtacggt tggactcatt ggccaccagc aggccgccgc atacgtcgga gtcttgggag
     8221 accagcaggg cggtgaggcg aggcaagcga cgacgctttc gagcacgcgg gtgcagtgca
     8281 ggtggcttct gcgctgcagc cgacccaata cacgctgtcg ctctggcgca tgtttgcttc
     8341 cgaatcaatt ccgcgccggc attgggtcgg tcgcgccacg tcgactcctc gacgaacggg
     8401 cgatcgagcc acttttcgtg cccgctggtg tccccgttct tcgaatgtcg gctctggcga
     8461 tttcggcact gcgcgcaacc gtgcgccacg tctgctccgc ggcgtgaccc gcaattccga
     8521 atgtcgcatt acgagcgcat caatgcctgc acatggccgc aagccgccgt cgcccttcca
     8581 acctacccgc gcgacaccag cgcgtcccag atcaccgccg cgacgatgct ccaggctccc
     8641 ggcgcctggt cgattctctc gttcaccagt tcggcaacgg tcgactgcga gacgacgccc
     8701 gtatcgttga atcgaagacg ggcgatcggt gtcgcttccg tcgcccccgc ggagtccatc
     8761 cgtcggcact cgacttcggc gcatggcggc tggtcagcgc catgtagaac gagtacgaac
     8821 tcgacgcgcg agccgctgtg cacgacgacg aagcggtcgg ccttggggtc gtgcgcgacc
     8881 cgcaagccgc gtagcagcgg tcgtccgtcg ccggcttgtt cggccagctt cgcgaacgag
     8941 cggaacaggc cgcgcaggat ggacgcggcg ccggtgtcgt cggcttcgac cttcttcctg
     9001 gcgactctct tcattcttgt ctcgggttac accgatgttg gcgagcctgc gatcgtctca
     9061 cttccgctgg ccgcgtcact tctccggcgg cttcccggcc agtcgcacct tgagatccgc
     9121 gcagggtgtg tcggtcgaca cgcgatcgcg gctggaagaa ttgcctgtcc cgttcgcgtc
     9181 cagttgccgc tggtgttcat tgtcgtgacg gtacaggcac agcggctccc agatgctgcc
     9241 atcggacggg ttgttgtcgt ggtcgtggtc gcggtggtga accgtcagtt cgcgcgcgtt
     9301 ggcgcgcgtg aactcgcgcg caatgcccgc agatcgtccc ggtgcggatc atccgcccga
     9361 cgacagcgct actccgcctg cgccggaatc gagcgcaccg acagcacgcc gtagatcgcg
     9421 gcgagcgcgt cgaccacctt ctgctccacc gggctgtcga cgtcgaccag cgtgtaggcc
     9481 atctcgccgc gcgac
//
LOCUS       DBNHBY010000571         3962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_917746, whole genome shotgun sequence.
ACCESSION   DBNHBY010000571 DBNHBY010000000
VERSION     DBNHBY010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_917746"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..864)
                     /locus_tag="AC16HB_13930"
     CDS             complement(190..864)
                     /locus_tag="AC16HB_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5607934.1"
                     /translation="MSAILRKEIDLQFKVSLLACTVVGWTAVAGTARAADDMGPYIGA
                     SIGHSHLTAGGDKIDRAFTAQGLTTSTALDRNDTAYALTLGYRVNPYLAVETNYVDFG
                     KYSLNSAVSAPAADTLRGRFKADGFDLVAVGILPLEGGFSAYGKLGALWSKAKLDAAS
                     TGAVSVSNTSHSNTGATFGLGLGYDLNRNVSTSLEWNRYDRIGEASSTGSTNFDLYTV
                     GIKYRF"
     gene            1046..1750
                     /locus_tag="AC16HB_13935"
     CDS             1046..1750
                     /locus_tag="AC16HB_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607935.1"
                     /translation="MTSRILVVDDDRGLRDLLCSYLGEAGHEVVACADGAAMWVHLER
                     DRFDLLVLDLMLPGDDGLTLCRHLRARSNLPVLMLTARGDEVDRVLGLEMGADDYLSK
                     PFSPRELHARIKSILRRVANREPAGAARVAFAGWTLDLAAHSLRDPYGVVVPLPTGEF
                     RLLRALAENLNRVQTRDQLIDALAGREASPVDRTVDVLVSRLRRRLGDDAREPKLIRT
                     VRSEGYVLVSNLGCEF"
     gene            1747..3189
                     /locus_tag="AC16HB_13940"
     CDS             1747..3189
                     /locus_tag="AC16HB_13940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5607936.1"
                     /translation="MKLLPRTLFGQIVVALLVGIVAAQAVGAWLMLDERTRLAQRLLG
                     THAAQRIAGIIDALDAADATERARLVHALSLPPTRIRVDEPWHPSAAADEEANAFADE
                     VRRLLPHPLTIQVLSLRGEAFPGWHPSRDIPPGALPEPRFDVGRRPDSGLPVPGEPAR
                     PHPRMLIRAELQARLSDGTVVTFSHALPGPLPEDPLRILGWVGVVLAIVAALALWIVR
                     RLTRPLRTLAAAAAGLPTNLDQPPLPESGPSEVVSAARAFNAMQRDLRRMIETRTVAL
                     AAVSHDLRLPLTRLRLRLEGLRDGAQRASIESDLADMERMIDNTLDFLRSGRTTETPV
                     LLDVNALLDRIAEDSSALGADVAVQGRAQPLLVRPQALERCLANLVDNARRYGGSHVD
                     VTVDDSPGRVEIRIEDRGPGIDEVERERVFEPYVRLETSRARETGGTGLGLAIARAVA
                     RLHGGDVQLRPRPGGGLSAVLTLARVPGVA"
     gene            3335..3595
                     /locus_tag="AC16HB_13945"
     CDS             3335..3595
                     /locus_tag="AC16HB_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014273.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR5607937.1"
                     /translation="MRPIRVCCTDIRPDPASPGYRPQQVEVLAESIRAHGVLRPILLR
                     EEANGYVIVHGERRWRAACALGHATIIAFLVIDLVHRSGAVL"
     gene            complement(3608..>3962)
                     /locus_tag="AC16HB_13950"
     CDS             complement(3608..>3962)
                     /locus_tag="AC16HB_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5607938.1"
                     /translation="PPSTSIVTALGLACGMGFGTVMPTAQLTIQTLAGRDKLGAAAAV
                     VSLSRSTGAVLGTALFGALVYGLLHGVDLDAALRDAFANRDAIVRAFQRGFIAAAMLA
                     AAAAVVASRLPPLRL"
BASE COUNT          592 a         1324 c         1401 g          645 t
ORIGIN      
        1 cctgcgccgg ggtggcaggg tcatgccgct caagcgcgcg attctgaagc tcatcgtgtc
       61 gagcctcttc cccggcgccg ggcgtgtgcg cgctcggcac gccggccgac cgccgaagga
      121 cgaacgcgac tggagggggt agtgcggtgg cccttcggcg gccgacgccg acgttcggac
      181 ggtgcccgtt cagaaacggt acttgatgcc caccgtgtag agatcgaagt tcgtgctgcc
      241 cgtgctgctg gcctcgccga tgcggtcata gcgattccac tcgaggctgg tggagacgtt
      301 gcgattcagg tcgtagccca gccccaatcc gaaagtcgcg ccggtgttcg aatgactcgt
      361 gttcgacacg ctgacggcgc cggtcgaggc ggcatcgagc ttcgccttcg accacagtgc
      421 gccgagtttg ccgtaggccg agaagccacc ctcgagcggc aggatgccga ccgcgaccag
      481 atcgaatccg tcggccttga acctgccgcg cagcgtgtcg gcggccggcg cgctcaccgc
      541 cgagttcagg ctgtacttgc cgaagtcgac gtaattggtt tccaccgcca ggtacgggtt
      601 gacgcgatag cccagggtca gcgcgtacgc cgtgtcattc cgatcgagcg cggtggacgt
      661 ggtgagcccc tgcgcggtga acgcgcggtc gatcttgtcg ccacccgcag tcaggtgcga
      721 atggccgatg ctggcgccga tgtacggacc catgtcgtcg gccgcgcggg ctgttcccgc
      781 cacggcggtc caaccaacga cggtgcaggc aagcaatgaa accttgaact gcaagtcgat
      841 ctccttgcgc aggattgcgc tcaacgggtg tgcagagaat cgatcctctg cataccgcca
      901 ttggagcgcg ccggtgtgaa ctgatttcgc cgcggcgggt cggatttgtg aaccggtcgt
      961 aacagatggt catcaaatgt gtttgcagaa gcgacgggcc cggtctggcc gctatcctcc
     1021 ggcgcagccg atctgctgga ccgcaatgac ttcacgaata ctcgtcgtcg acgacgaccg
     1081 cgggctgcgc gacctgctgt gcagttacct cggcgaagcc ggtcacgagg tggtcgcgtg
     1141 tgccgatggc gcggcgatgt gggtgcacct ggagcgcgac cggttcgacc ttctggtgct
     1201 cgatctgatg cttccgggcg acgacggcct gacgctgtgc cgccacctgc gcgcgcgttc
     1261 gaacctgccg gtgctgatgc tgaccgcgcg cggcgacgag gtggaccgtg tgctcgggct
     1321 cgaaatgggt gccgacgact acctttcgaa accgttcagt ccgcgcgaac tgcatgcacg
     1381 catcaagagc atcctgcgcc gcgtcgccaa tcgcgagccg gccggagccg cgcgcgtcgc
     1441 cttcgccggc tggacgctcg acctcgccgc gcacagcctg cgcgatccgt atggcgtggt
     1501 ggtgccgctg ccgaccggcg agttccggct tctgcgcgcg cttgccgaga acctcaatcg
     1561 cgtgcagacg cgtgatcagt tgatcgacgc gctcgcgggg cgcgaagcga gcccggtcga
     1621 tcgcacggtc gacgtgctcg tgagccgcct gcggcgccgc ttgggtgacg acgcgcgcga
     1681 gccgaagctg atccgcaccg ttcgcagcga ggggtacgtg ctggtctcga acctggggtg
     1741 cgaattttga agctgctgcc gcgcacgctg ttcggacaga tcgtcgtcgc tctgctggtg
     1801 ggcatcgtcg cggcgcaggc cgtcggcgcg tggctgatgc tcgacgagcg cacgcggctg
     1861 gcgcagcgcc tgctcggcac gcatgcggcg cagcgcatcg ccggcatcat cgatgcgctc
     1921 gatgcggccg atgccaccga gcgcgcgcgc ctcgtgcacg cgctgagcct gccgccgacg
     1981 cgcatccgcg tcgacgagcc atggcatccg agtgccgccg cggacgagga ggcgaacgcc
     2041 tttgcggatg aggtgcggcg actgctgccg catccgctga cgatccaggt gctgtcgctg
     2101 cgcggcgagg catttccggg ctggcacccg tcgcgggaca ttccgccagg cgcgctgcct
     2161 gagccacggt tcgacgtcgg caggagaccg gattcggggt tgcctgtgcc cggcgaacct
     2221 gctcggccgc atccgcgcat gctgatccgc gccgaactgc aggcacggct gtccgacggc
     2281 accgtcgtca ctttttctca cgcgctgccc gggccgctgc cggaggatcc gctgcgcatt
     2341 ctcgggtggg tcggcgtcgt gctggcgatc gtcgcggcgc tggcgctgtg gatcgtgcgg
     2401 cgcctgacgc gtccgctgcg caccctggcc gcggccgcag ccggcttgcc gacgaacctc
     2461 gatcagccgc cgttgcccga atcgggtccg agcgaggtcg tcagtgccgc gcgcgccttc
     2521 aatgcgatgc agcgcgacct caggcggatg atcgaaacgc gcacggtggc gctggccgcc
     2581 gtttcccacg acctgcggct gccgctgaca cggctgcggt tgcgcctgga gggtctgcgc
     2641 gacggcgctc agcgcgcgtc gatcgagtcc gaccttgcgg acatggaacg catgatcgac
     2701 aacacgctcg atttcctgcg cagcggccgc acgaccgaga ccccggtgct gctcgacgtc
     2761 aacgcgctgc tcgatcgcat cgcggaggac agcagcgcgc tcggcgccga cgtggccgtg
     2821 cagggcaggg cgcagccgtt gctggtgcgt ccgcaggcac tggagcgctg ccttgccaat
     2881 ttggtcgaca acgcccggcg ctacggtggg tcgcacgtgg acgtgacggt cgacgattcg
     2941 ccgggtcggg tcgagatccg catcgaggat cgtggaccgg gaatcgacga ggtcgagcgc
     3001 gagcgtgtgt tcgaacctta cgttcgactg gagacgtcgc gcgccaggga gaccggcggc
     3061 accgggctcg gactcgcgat cgcgcgcgcc gtggcgcgcc tgcacggcgg cgatgtgcag
     3121 ttgcgcccgc gacccggcgg cggcctgtcg gcggtgctga cgctggcgcg tgtccctggg
     3181 gtcgcttgaa aaggtgcgta cgccggccac gccgatgcag gccaacgcgc agatcgagcg
     3241 cattcacaaa aatgcggcag cgacgatttg tcaggcgtta acacggaccg cgtaggtttg
     3301 cgggcggtgt ccgatcgatc agaaggagtg agcgatgcgt ccgatccgcg tgtgctgcac
     3361 cgacatccga cccgatccgg ccagtccggg ctatcggccg cagcaggtcg aggtcctcgc
     3421 cgaaagcatt cgcgctcatg gcgtgcttcg gccgatcctg ttgcgcgagg aggcgaacgg
     3481 ctacgtgatc gtgcacggcg aacgccgctg gcgcgccgcg tgcgcgctgg gtcacgccac
     3541 gatcatcgcg ttcctggtca tcgacctggt gcacaggtcg ggcgcggtcc tgtagcccgc
     3601 gccgcgccta caggcgcaac ggcggcaatc gcgacgcgac cacggcagcg gccgcggcga
     3661 gcatcgccgc tgcgatgaag ccgcgctgga acgcgcgcac gatggcgtcg cgattcgcga
     3721 acgcatcgcg caacgcggcg tcgagatcga cgccgtgcag cagtccgtag accagcgcac
     3781 cgaacagcgc ggtgccgagc actgcacccg tcgaccgtga cagcgaaaca accgccgcgg
     3841 cggcgccgag cttgtcgcgg ccggccagcg tctggatcgt gagctgcgca gtcggcatca
     3901 ccgttccgaa acccatcccg cacgcgagtc cgagcgcagt cacgatcgag gtcgacggcg
     3961 gc
//
LOCUS       DBNHBY010000572         3564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_783603, whole genome shotgun sequence.
ACCESSION   DBNHBY010000572 DBNHBY010000000
VERSION     DBNHBY010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_783603"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC16HB_13955"
     CDS             complement(<1..457)
                     /locus_tag="AC16HB_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607939.1"
                     /translation="MLPEPVQASSHAPSPTHQHARQLRILLADDHEMMRLALRTALAP
                     LSPAIEWFEAGRADDTRALLEGRPDIDLALIDFNMPGASGIQWLADLRRRHPSLPIVV
                     MSAQEDTELVRQLIALGIAGFIPKTDSARVVLQAVTLVLSGGTYAPLRLL"
     gene            595..2931
                     /locus_tag="AC16HB_13960"
     CDS             595..2931
                     /locus_tag="AC16HB_13960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5607940.1"
                     /translation="MAVAMAAAVAAPGASDRFLVRLTQAELEHIAPDGTVVGPAESFT
                     LPGHWRGNDAGYATRHRFDVRVPPGHRELSIFVEAARLHFSARVNGTEVFATTRGDAR
                     ASPRLRSWRMSPQFSVPPELLVEGVNHIDLISFAPPHRGGGLAPVWIGDSDAVAKVDF
                     RIFVRDTVGPLVIAAVIATLGVFALVLARGQRDAATFVLFGVGALLWGAHTAVTLMPE
                     TPLPIEHYVVLWYAAYVWFTSALSLFCIRFAGLQWRWFSRFCAAFSLAALPIMYGIVL
                     LGAGAIAYGIFLFACMAIAIVALFGVMRFALRSRSFDGFMLLAVGVLSVSFSIYDWLK
                     SNVWGELVKEYILTPYAGLAFICLAGWMLIDRYQRTAGALEELNRTLERRVKDANLEL
                     QRQLDEVRAAREVAEQANVAKSKFFAAASHDLRQPLHSLGMFASALDAHLDSDEGRSL
                     RRRIGSSIEVLEGLFNELLDLSRLDAGVIEARPQPVKLQELFDRLDDQFSREAQERGL
                     NLRFVPTRLAVTSDPVLLERILANLVTNALRYTERGGVLIGTRRRGGEVVIEVRDSGI
                     GIAASDQQRIFDEFYQVNNPARDRRKGLGLGLAIVRRLTELLGHSLQLRSAPSRGTVF
                     GLTVPRCDTLPAAPARGDRFPLPSLLQDKCVLVVDDEIEIRDASVALLGQWGMQTIAV
                     ADPAEFASALESGAKPDAAIVDLRLGDAIDGVDIIALLRARFGARFPALLISGDTGAR
                     ELARVKASGLSLLIKPVQPARLKAALHACLSGSLSSAQ"
     gene            complement(2920..>3564)
                     /locus_tag="AC16HB_13965"
     CDS             complement(2920..>3564)
                     /locus_tag="AC16HB_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR5607941.1"
                     /translation="PRMAQIYGQGESPMTITVLPVADHFDDHDGRRIERLGSVGRAQT
                     GMEVAVFGNDDRPLAAGEIGEVVVRGDTVMRGYLNDPEATARTLAHGWLHTGDLGRFD
                     SHGYLTLVDRSKDLIISGGSNIYPREVEEVLLKHPGVAEVAVIGEKDADWGEAVVAVV
                     VARADAQAAAPALDAFCPQHIARFKRPKRYVFVAELPKNAAGKVLKRELRLSLR"
BASE COUNT          544 a         1151 c         1206 g          663 t
ORIGIN      
        1 tgagcaacct gagcggggca taggtgccgc cggacagaac cagggtgact gcctgcagca
       61 ccacccgcgc cgaatcggtc ttcgggatga accccgcgat gccgagcgca atcagctgcc
      121 gcaccaactc ggtgtcttcc tgcgccgaca tcacgacgat gggcagcgaa ggatggcggc
      181 gccgcagatc ggccaaccac tgaatgccgc tcgctccggg catgttgaag tcgatcagcg
      241 cgagatcgat gtcgggccgt ccctcgagca gcgcccgggt gtcatcggcg cggccggcct
      301 cgaaccattc aatggcgggg ctgagtggag cgagcgccgt tcgcagtgcc agccgcatca
      361 tttcgtgatc gtcggcgagc agaatgcgca gttgccgcgc gtgctgatgc gttggcgaag
      421 gagcgtgtga agaggcctgt acgggttccg gcagcacggg taaggtgacc gttttttctc
      481 agggaccgct gttcgctggc ttccggacat tgatcgacca gccggcaacc atggttgcgg
      541 aatctaatga cgacgcgcgc acaccgggtt gtggatgcct tgattgcgct agccatggca
      601 gtcgcgatgg ccgcggcggt ggccgcgccc ggagcgtctg accgcttcct ggttcggctg
      661 acacaggcag aactcgagca catcgcgccg gacgggaccg tcgtcggtcc cgcggagagt
      721 ttcacgctgc ccggccactg gcgtggcaac gacgcaggct acgcgacgcg tcaccgcttc
      781 gacgtgaggg tgccgcccgg acaccgcgaa ctgtccatct tcgtcgaggc ggcgcggctg
      841 cacttcagcg cgcgcgtcaa tggcaccgag gtgttcgcca ccacacgcgg cgacgcgcgc
      901 gcgtcgccgc gactgcgcag ctggcgcatg tcgccgcagt tcagcgtgcc accggaactg
      961 ctggtcgaag gcgtgaacca catcgacctg atcagtttcg cgccgccgca ccggggcggt
     1021 ggacttgcac ccgtctggat cggggattcg gacgctgtcg cgaaagtgga ctttcgaatc
     1081 ttcgtacgcg atacggtcgg gccgctcgtg atcgcggcgg tcattgcaac gctgggcgtg
     1141 ttcgcgctcg tgctcgcccg cggtcagcgt gatgcggcga ccttcgtcct gttcggcgtc
     1201 ggtgcgctgc tctggggtgc gcacaccgca gtgacgctga tgcccgagac gccgcttccg
     1261 atcgaacact atgtcgtcct ctggtatgcc gcctatgtgt ggttcaccag cgcgctgagc
     1321 ctgttctgta tccggtttgc agggttgcag tggcgctggt tctctcgatt ctgcgctgcg
     1381 ttcagcttgg ctgcgctgcc gatcatgtac ggcatcgtgc tcctcggtgc cggcgcgatc
     1441 gcctatggaa tcttcctgtt tgcctgcatg gcgatcgcga tcgttgccct gttcggcgtg
     1501 atgcggttcg ctctgaggtc gcgcagcttc gacggattca tgctgcttgc cgtgggcgtg
     1561 ctgtcggtgt cgttctcgat ctacgactgg ctgaagtcga atgtctgggg ggaactggtc
     1621 aaggaataca tactgacgcc gtatgcggga ctcgcgttca tctgcctggc cggctggatg
     1681 ctgatcgatc gttaccagcg cactgccggc gcgctcgagg agctgaaccg gacgctggag
     1741 cgccgcgtga aggacgcgaa tctcgaactg cagcgccaac tcgatgaggt ccgtgcggcg
     1801 cgtgaggtgg ccgaacaggc caatgtggcg aaatcgaaat tcttcgctgc cgccagccac
     1861 gacctgcgcc agccgctgca ttcgctcgga atgttcgcgt cggcactcga tgcgcacctg
     1921 gactccgacg agggccgctc gctgcggcgg cgcatcgggt cctcaatcga ggtgttggaa
     1981 ggactgttca acgaactgct cgacctgtcg cggctcgatg ccggcgtgat cgaggcgagg
     2041 ccgcagccgg tgaagctgca ggagctgttc gatcgcctcg acgaccagtt cagccgcgaa
     2101 gcgcaggagc gcggcctgaa tctgcgcttc gtgccaacgc gcctggctgt gaccagcgat
     2161 ccggtcctgc tggaacggat cctcgccaac ctggtcacca atgcattgcg ctataccgag
     2221 cgcggcggcg ttctgatcgg cactcgaagg cgcggcggcg aagtcgtcat cgaagtgcgc
     2281 gattccggca ttggaattgc tgcttccgac cagcagcgga tcttcgacga gttctaccag
     2341 gtcaacaacc cggcgcgcga tcgccgcaag ggcctcgggc tcgggctggc aatcgtgcgc
     2401 cgactgacag aactgctcgg gcactcgctg cagctgagat cggcgccatc gcgtggcacc
     2461 gttttcggcc tgacggtgcc gcgttgcgac acgctgcccg ccgcgcccgc gcgcggcgat
     2521 cgattcccgc tgccgtcgct gctgcaggac aagtgcgtgc tggtcgtcga cgatgaaatc
     2581 gagatccgcg atgcatcggt cgcgctgctc ggacaatggg gcatgcagac gatcgccgtc
     2641 gccgatcccg ccgagttcgc atcggcactg gaatccggcg cgaagccgga tgcggcaatc
     2701 gtcgacctgc gcctgggcga tgcgatcgac ggcgtcgaca tcattgcgct gctgcgcgcg
     2761 cggttcggcg cgcgttttcc ggcgctgctg atctcgggcg acaccggtgc gcgtgaactg
     2821 gcacgcgtga aggcgagcgg cctgtcgttg ctgatcaagc cggtgcagcc cgcgcggctg
     2881 aaagcggcgt tgcacgcgtg cctgtccgga tcgttgtctt cagcgcagtg aaagacgcaa
     2941 ttctcgcttc agcacctttc cggccgcgtt cttcggcaat tccgcgacga acacgtagcg
     3001 ctttggccgc ttgaagcggg cgatgtgctg cgggcagaac gcgtcgagcg cgggcgcagc
     3061 cgcctgcgcg tcggcgcgcg cgacgactac cgcgaccacc gcctcgcccc agtcggcgtc
     3121 cttctcgccg atcacggcga cctcggccac gcccgggtgc ttgagcagca cttcctcgac
     3181 ttcgcgcggg tagatgttgg aaccgcccga gatgatcagg tccttgctgc gatcgacgag
     3241 cgtcaagtag ccgtgtgaat cgaagcggcc cagatcgccg gtgtgcagcc agccatgggc
     3301 gagcgtcctg gcggtcgcct cgggatcgtt caggtagccg cgcatcacgg tgtcgccgcg
     3361 cacgacgact tcgccgatct cgcccgcggc gagcggtcgg tcgtcgttgc cgaacaccgc
     3421 gacctccatg cctgtctgcg cgcggccgac cgagccgagc cgctcgatcc gtcgcccgtc
     3481 atggtcgtcg aagtgatcgg cgaccggcag cacggtgatc gtcatcgggc tttcgccctg
     3541 gccgtagatc tgcgccatcc tggg
//
LOCUS       DBNHBY010000573         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1594369, whole genome shotgun sequence.
ACCESSION   DBNHBY010000573 DBNHBY010000000
VERSION     DBNHBY010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1594369"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..983)
                     /gene="proC"
                     /locus_tag="AC16HB_13970"
     CDS             complement(162..983)
                     /gene="proC"
                     /locus_tag="AC16HB_13970"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR5607942.1"
                     /translation="MNPPFRIGFIGGGNMAGALIGGLLRNGADAAVITVVEIDAARRA
                     QLQREFGIATRPAPDASLATADAIVLAVKPQQMKDVCDTLRPSIGVQLVLSIAAGIRA
                     ADLARWLGTQCIVRAMPNTPALVGRGITGVAALGGVSEAQLQLAERILGAAGSVVRVP
                     DDAALDAVTALSGSGPAYVYYFIEALVAAGVEMGLTAEQSRRFAVETVAGAAHLASST
                     DEPLEVLRARVTSKGGTTAAAIARMDADKIKRKIVAAVLAARARANELGDEYGKN"
     gene            complement(980..>1552)
                     /locus_tag="AC16HB_13975"
     CDS             complement(980..>1552)
                     /locus_tag="AC16HB_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607943.1"
                     /translation="APEHVCAALARLSTLNWIADAAIHAVEFGQLAQQALGFDRTAVD
                     ASFASTFNNVTSRLLAASPPAPVEAPLRGALLHGAEAARAFWYRAGQWLERERAHYLC
                     AKVALRIGDHGRARAAARHGLALVEANGNDVVERAFLLQPLAAALIRSGDAASAPAVR
                     AEADSLAATFDTDLRNYYAMDTSELFGLTS"
BASE COUNT          209 a          565 c          546 g          232 t
ORIGIN      
        1 gtacattgcc gggtggctcg gtgcgctcgg ctgtgcgctc tatcacttcc tgctgatccg
       61 cggccgcgcc cgcgacgcgt gcttccgcgc gttccggcac aaccagtggt tcgggctggc
      121 gatcttcgcc ggcgtggccg ccgactacgc gctgcgctga ttcagttctt gccgtactcg
      181 tcgccaagct catttgcgcg cgcacgcgcg gcgagcaccg cggcgacgat cttgcgcttg
      241 atcttatccg cgtccatgcg cgcgatcgcg gcggccgtcg tgccgccctt ggatgtgacg
      301 cgtgcgcgca gcacctccag cggctcgtcc gtactgctgg ccagatgcgc ggcgccggcg
      361 acggtttcga ccgcgaagcg gcgcgactgc tcggccgtga ggcccatctc gacgccggcc
      421 gcgacgagcg cctcgatgaa gtagtacaca taggccggcc cgctgcccga cagcgcggtc
      481 accgcgtcga gcgcggcgtc gtcggggacc cggaccacgc tgccggccgc gccgagaatg
      541 cgctccgcga gctgcaactg cgcctcgctg acgccgccga gcgcggccac gccggtgatg
      601 cccctgccga ccagcgccgg cgtgttcggc atcgcccgta cgatgcactg cgtgccgagc
      661 cagcgtgcca gatccgcggc gcggataccg gcggcgatcg agagcacgag ttgcacgccg
      721 atcgacggcc gcagcgtgtc gcacacgtcc ttcatctgct gcggcttgac cgcgagcacg
      781 atcgcgtcgg cggtcgcgag cgaggcgtcc ggcgccggcc gcgtggcgat gccgaactcg
      841 cgctgcagct gcgcgcgccg cgccgcgtcg atctccacca cggtgatcac cgccgcgtct
      901 gcgccgttgc gcagcagccc gccgatcagc gcgccggcca tgttgccgcc gccgatgaag
      961 ccgatgcgga agggcgggtt catgacgtga gaccgaacag ttcgctggta tccatggcgt
     1021 agtagttgcg caggtcggtg tcgaaggtcg ccgcgagcga atcggcctcc gcgcgcactg
     1081 ccggcgcgct ggccgcgtcg ccggaacgta tcagcgcggc ggccagcggc tgcaacagga
     1141 aagcgcgttc gaccacgtca ttgccgttgg cttccaccag cgccagtccg tggcgggccg
     1201 cggcgcgcgc gcgtccgtgg tcgccgatgc gcagcgcaac cttggcgcac agatagtggg
     1261 cacgctcgcg ttcgagccac tgcccggcgc gataccagaa cgcgcgtgcc gcctcggcgc
     1321 cgtgcaacag ggcgccccgc aacggtgcct cgaccggcgc aggagggctc gccgcgagca
     1381 gccggctcgt gacgttgttg aacgtcgacg cgaagctggc gtcgacggcg gtccgatcga
     1441 agccgagcgc ctgctgcgcc agctgtccga actcgaccgc gtgaatcgcg gcatcggcga
     1501 tccagttcag cgtcgacaga cgcgccagtg cggcgcacac gtgctccggt gc
//
LOCUS       DBNHBY010000574         8318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_715189, whole genome shotgun sequence.
ACCESSION   DBNHBY010000574 DBNHBY010000000
VERSION     DBNHBY010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_715189"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="AC16HB_13980"
     CDS             complement(<1..688)
                     /locus_tag="AC16HB_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR5607944.1"
                     /translation="MPTAVFVSAEDHMRRYGRDAVGVYVDGVDGRLMRSIKSILGSDL
                     MDETTEIAPGLTAGYVDIVIGYLRHLRVVAQRQAGRELSRVVLGRPVFFVDDDAGRDA
                     RAQDTLAAAAHAAGFDEVSFQFEPIAAALDYESRLDRERLVLVADIGGGTSDFSLVRA
                     NPDRHARLDRRADILANHGVHIAGTDFDRAVNLRAIMPALGYGSRGPHGRTVPGRIYH
                     ELATWHLINTT"
     gene            1028..1180
                     /locus_tag="AC16HB_13985"
     CDS             1028..1180
                     /locus_tag="AC16HB_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607945.1"
                     /translation="MARLRPSATDLVTASKLPLADCRHSPHRDQPDAVLAMVDTFVTR
                     VLDARE"
     gene            1537..2973
                     /locus_tag="AC16HB_13990"
                     /pseudo
     CDS             1537..2973
                     /locus_tag="AC16HB_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112390.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            3352..4392
                     /locus_tag="AC16HB_13995"
     CDS             3352..4392
                     /locus_tag="AC16HB_13995"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5607946.1"
                     /translation="MNPAAHWSEGASRATLRRRDSVPLRRTFLLVSGLCLIGAARASR
                     AQTVATPRKVGILALGSESDHAASVWAFRERLVELGYVEGRNLVLESRYGRSEALPVL
                     AAELIRLQVDVIVTISTPAAQAAKAATASIPIVMAGSANPVELGLVASLSHPGGNVTG
                     VTHHVTPGFAVKQLQLLKEAVPKISRVAIIKVDHPIEDAAFGAMELAAPQLGVVPVAV
                     VIDASNPSDVRLPLTQARPDALFVFPNSTNDFYAGAIAAFAAENRLPTMYGNRGGPHA
                     GGLLSYGVNWLDLRRHAAVYVDKILRGRKPADLPVEGPVKFQMIVNLKTAKTLGITIP
                     QSVLLQATEVIE"
     gene            4399..5070
                     /locus_tag="AC16HB_14000"
     CDS             4399..5070
                     /locus_tag="AC16HB_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5607947.1"
                     /translation="MKVLILDDHALVRDALSRVLASLVPGVVILEAADPASAFVLVER
                     EPDIDLVLLDLALPGMHGLRALRKLRESYPAVSVVVVSATVDRDAILSAIDSGALGFI
                     PKSSSNEVLRSALQLVLSGGVYLPPEILERSQGAAPIESSPGGHKMPSEIGLTQRQAQ
                     ILAYIMRGDSNKLICRALNIAEATVKNQVTVILKALNVTSRTQAVLAVAKLGLVLPRT
                     GQDES"
     gene            complement(5055..7319)
                     /locus_tag="AC16HB_14005"
     CDS             complement(5055..7319)
                     /locus_tag="AC16HB_14005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5607948.1"
                     /translation="MTGRSIATRYAALVVALLCSALTIGGAVEIWTAHRERLAVLEAL
                     QLEKAQSAAQAVSRVFDDLRRSLDWATLGAAKGAGNELTLRRLELLKLLRLEPAVTAA
                     TLVDRSGIEQVHVSRIEPDRVASGVDWSNDSDFTVARAGRSAFSSVQFVGQTEPHLTM
                     AVPSAGRDGGVILADVNLKFVWNVISRIQVGQSGFAYVVDKQGRLVSHPNISFVMQMT
                     DLSRLPQVEAALAVQGQGFDAAGRDQNGVPVLAAHASIAPLAWTVFVEQPRAEAFVPL
                     AASVMRGALILLLALAMALAAGVLAARRMVAPMGELRRGAERFGSGDLSHRIDIASGD
                     ELQELADRFNAMAERLGQTYAELERRVAERTRQLDLANRAQTRFLAVASHDLRQPMHA
                     MALFVDQLRTTRSADEHAALIQRVEDAARILSDLLDQLLDLSRLEAGLLQAAPIDFDA
                     ASVLTPIEAQFAPLARAKGIEIRVHTAHAWLRSDPVLVQRILMNLVANAIQCTERGGV
                     LFGVRRRAGLLRLAVWDTGRGIPQDQRDEVFREFVRLGHAGAQPQRGLGLGLAIVARL
                     VEVLGTRVELQSTLGRGSVFWFDLPSGTATAVPPPAVKPALRADLRGTFVCVIDDEEP
                     ARTAMCSLLERWGCLTLAAASGDEALAQLRAHDRPPELIVCDFRLAAGEDGLALIARM
                     RAAIAELVPALLVTADTTAETAAAARAAQVPLLHKPTSPIKLRALVTRLLERSGERDW
                     AVHEPPSGQAQLSS"
     gene            7530..>8318
                     /locus_tag="AC16HB_14010"
     CDS             7530..>8318
                     /locus_tag="AC16HB_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5607949.1"
                     /translation="MSTTTKALTFAALAALALSAWAQQGTLDKIKASGAITIGHPNVI
                     PFAYRADGPQPVGFAIDLCARVVDAIKAKLKLPELKVNYYRETTTPATRIPLLVDGTI
                     DLDCGASTNTAERQKQVAFSMTYFLSVTRFIAKKDDNLTKLADLKGKTVASQLSTPAL
                     KQITELNAAQNLGLNILVVNYGREVMPTLESGRAVAFVYDDVLLAGFAANHADPGLWQ
                     ISNEALSLPEPYGIMLRKDDPEFKKAVDAAMVATYKSGEAARLYA"
BASE COUNT         1556 a         2682 c         2695 g         1385 t
ORIGIN      
        1 acgtcgtgtt gatcaagtgc caggttgcaa gctcatggta gatgcggccg ggcacggtcc
       61 ggccgtgcgg accgcgcgag ccgtaaccga gcgccggcat gatcgcgcgc aggttcaccg
      121 cgcgatcaaa gtcggtgccg gcaatatgga caccgtggtt ggcgaggatg tcggcgcggc
      181 gatcgagcct tgcgtgccga tccggattgg cgcgcaccaa tgagaaatcc gaggtgccgc
      241 cgccgatatc ggcgaccagc accaagcgct ctcggtcaag ccgcgattcg tagtcgagcg
      301 cggctgcgat cggctcgaac tgaaaggaca cttcatcgaa gccggctgcg tgggccgcgg
      361 cggccaaagt gtcctgcgcc ctcgcgtcgc gacctgcatc gtcgtcgacg aagaagaccg
      421 ggcgcccgag caccacgcgg ctgagttccc gccctgcttg acgctgcgcg acgacgcgca
      481 ggtgccgcag gtaaccgatc acgatgtcga cgtaaccggc cgtgagtccg ggcgcaatct
      541 cggtggtctc atccatcagg tccgagccca gaatgctctt gatcgaccgc atgaggcgac
      601 cgtccacacc atcgacgtac acgccaaccg cgtcgcggcc gtagcggcgc atatgatcct
      661 cggcgctgac gaacacggcg gtcggcatgt tgctgaaacc gggctccagt tcggcgaggc
      721 gaattccggt ggtcgacgga taggcgagcg cggaattgga cgtgccgaag tcgattgcgc
      781 agtaatccac gcgggctccc ggcttcaacg aaaataagtg tccacagcgc aactggagag
      841 aagcatcgcg gacacggcgg gtcgatcata gaagtgcgcc cgaacgccgc gaggtcgcgt
      901 tgcagcggcc cgcatccgct gaatctggta cgtcacctgc gactcgtcac gcccgtcggt
      961 cgacgaccgc aacaacgtcg gtccgccaag cgaagggcga cccgtgactt gaagctgtgc
     1021 gccgtgaatg gctcgcctcc ggccatcggc gaccgacctg gtcacggcct caaagctgcc
     1081 gctcgccgac tgccgccact cgccgcacag ggatcagccg gacgcggtgc tcgcgatggt
     1141 cgatacgttc gtgacgcgcg tgctggatgc gcgggagtga ctgggcagac ctgagtaacc
     1201 cgcggccaac tctccgtgca taaacgggcg cagtaggctg caaccggccg agcgatcatg
     1261 tctgccatga catcaagcaa tggcgtgctt tcgacccatt ccgggccgtc gagatcgtcc
     1321 gatcgattga cgggtttaca tcgctcacta gccattcgcc gcatggcgat gaaaggcgag
     1381 agccggggac agcggtcatc agagtgcgcg cctggcaaat cggtgactga cgggcagctt
     1441 tcgatgaacg cggccaccgc ctcacgaccg aggctgtgtg gaaacaccga cccgtgctct
     1501 gtgtcatggc gcccgttgga tcaacggggg tgatcgatga ggcgatacgt cgaaggcgaa
     1561 gatcgaacgc agagcgtgct gtttccggag cggttggatg actggatcga cgaggacagc
     1621 accgttcgag tcatcgatgt gcttgtcgat gaactcgacc tgcggcaact gggcttcggc
     1681 cgagttgagc ctgccgcgac gggtcgacct gcctacagtc ccgggacgct gctcaagatc
     1741 tacgtctgcg gttatctgaa tcgcattcag gccaaccgac gcctggaggc tgaagcgcgg
     1801 cgcaacctgg aactgatctg gttgaacggc cgcctcgcac ccgacttcaa gacgattgcc
     1861 gacttccgca aggacagcgg cgctgcgatc cgcaaggtct gcaaagagtt cgtcttgctg
     1921 tgtcgccggc tggatctgtt caccgacggc gtcgtggcca ttgacggcag caagttcaag
     1981 gcggtcaaca accgtgacaa gaacttcacc gaccgcaagc tacaggcgag gatcgagcgg
     2041 ctcgagcaaa gcatcaagcg ctacctggtc gaactcgacc gcgccgaccg cgagacgacg
     2101 gccgttctgc tgagccgggt ggctcatctg aaggagaaga tcgccaaggt caaggcgcag
     2161 atgcagatgc tgggcgagat cggtgaacag atgaaggtgt gcgaggacca tcaggtctcc
     2221 ctgaccgacc cggatgcgcg ctccatggcc accagcggcc ggggaacggg gatggtgggc
     2281 tacaacgtgc agaccgcggt cgacaccaag catcacctga tcgttgcgca tgaggtcacc
     2341 aacgtcggcc acgaccgtac tcatctgaca gagatggcga tccacacacg cgacgccgtc
     2401 gatcaggcaa agttgacggt gctcgccgat cgcggctgct tcaatggcga agagatcctc
     2461 gactgcgcgc gacaaggcat cacgaccctg gtgcccaagc cgctgacctc cggagccaag
     2521 gccgacggac gctttgataa acgcgacttc atcttcgacg ctgagcgcaa tgagtaccgt
     2581 tgcccagcga gcgagcgagc gctctggtgc atgaccacgg tcaagcgcgg gatgacgctg
     2641 catcgatact ggtcctcggc ctgcccacgg tgttgcatca agaagcaatg cacgccgagc
     2701 gactatcggc gtatctcgcg ctgggagcat gaagcggtgt tggaggccat gtaggatcga
     2761 ctggatcgaa ctcccgaggc ctcaaggctt cggcgccaga cagtcgagca cgtcttcggc
     2821 acgctcaagg cgtggagggg ctcatcgcac ttcctgacca agacgctgcc gcgggtccgc
     2881 acggaaatga gcctgcacgt gctggcctac aacatgaaac gagtgatgaa gatcctagga
     2941 acgcaaacgt tgatccagac catgcgggct tagaagttcg tccgccgtcg cccctcccat
     3001 tcactcggcc cgctacgtgc cggttcaacg cccagacggc acgcatatcg acttgcgcgc
     3061 tgcgtttcca cacggcctcg acccgaagca gctgcccacg ctgccgcaca agaacgcgaa
     3121 gagcggacac aagcgagagc tccggctcga gaagagctca gtgttcgtcg agccggcagc
     3181 aggcaaacaa cccccgccgc ggatccggtc agtggcggca cagtgatcgg caggttcata
     3241 gcacttgcct ctcctgcggg ggtagactga gcgccgatcc tccaaatttg caggccaagt
     3301 gagtcgccgc ggcacgttgg tcttttgtgc caatgacgag tcggcgccag gatgaatccc
     3361 gccgcgcatt ggtccgaggg tgcatcacgc gcaactttgc gcagacgtga ctcagtgccc
     3421 ttgcgaagga cattcctgct tgtctcaggt ctgtgcttga taggcgcggc gcgtgcttcg
     3481 cgggcgcaga ccgtagcaac gccgcgcaag gtcggcatcc tcgctctggg ctctgagtca
     3541 gaccatgcgg ccagtgtgtg ggcgtttcgc gaacgcctag ttgagttggg ctacgtcgag
     3601 ggccgcaatc tggttctcga atcgcgatac ggacgatcgg aggcgctgcc cgtgctcgcg
     3661 gcggagctga ttcggctcca agtggacgtc atcgtcacga tctccacgcc ggcggcgcag
     3721 gctgccaagg cggcgaccgc gtcaattccc attgtcatgg cgggaagcgc caatccggtg
     3781 gagctggggc tggtggcgag cctgtcgcat cccggcggca atgtgaccgg ggtgacccac
     3841 cacgtcaccc cgggattcgc cgtgaagcaa cttcagttgc tcaaggaagc tgtgcccaag
     3901 atctcacgcg tggcgatcat caaggtcgac catccgatcg aggacgccgc gttcggcgcg
     3961 atggagcttg ccgcaccgca gttgggcgtg gttccggtcg ctgtggtcat cgatgcctcg
     4021 aacccgagcg atgtgcgatt gccgctgacc caggctcgac ctgatgcgct gttcgtgttt
     4081 ccgaactcga ccaacgactt ctatgcgggt gcgattgcgg ccttcgcggc cgagaatcgc
     4141 ctgccaacga tgtacggtaa ccgtggtggg ccgcacgccg gcggcctgct gtcgtacggc
     4201 gtgaactggc tcgatctgcg ccggcacgcc gccgtctacg tggacaagat actcaggggc
     4261 cgcaagccgg ccgacctacc ggtcgagggc ccggtgaaat tccagatgat cgtcaacctc
     4321 aagactgcca agacactcgg catcacgatt ccccagtcgg tgctgctgca ggcgacggag
     4381 gtgatcgagt gaggtgcctt gaaggttctg atcctcgatg accacgcgct ggtgcgcgac
     4441 gccctgtctc gggtgctggc cagcctcgtg cccggagtcg tcatcctcga agcggccgac
     4501 ccggcgtcgg cattcgtctt ggtcgaacgt gaacccgaca tcgacctcgt gctgctcgac
     4561 ctcgcactgc cgggcatgca cggtctacga gcattgcgga aacttcgtga gtcctacccg
     4621 gccgtatcgg tggtggtggt ctcggccacc gtcgatcgtg acgccatcct gagcgccatc
     4681 gacagcggcg ctctcggatt catccccaag tcatcgagca acgaggtcct gagaagtgcg
     4741 ctgcaattgg tgctgtccgg cggtgtctat ctgccgcctg agatcctgga gcgcagccag
     4801 ggtgctgcac cgatcgagag cagccctggc gggcacaaga tgcccagcga gatcggtctc
     4861 acgcaacgcc aggcacagat cctcgcgtac atcatgcgtg gcgattcgaa caagctgatc
     4921 tgccgagcac tgaacatcgc ggaggccacc gtcaagaatc aggtcacggt gattctgaag
     4981 gccttgaatg tgaccagccg aacccaggcc gtgctcgccg tggccaagct gggtctcgtg
     5041 ctgccgcgaa caggtcagga cgaaagctga gcctggccgg atgggggttc gtggacggcc
     5101 cagtcccgct cgccggaccg ctcgagcaat cgtgtcacca gcgcgcgcag cttgatcggc
     5161 gaggtcggct tgtgcagcag cggcacttgc gccgctcgtg cggcggcggc tgtctcagcc
     5221 gtggtgtccg cggtgaccag cagcgccggc acgagttcag cgatggccgc gcgcatgcgc
     5281 gcgatcaggg ccagtccgtc ttcgccggcc gcaagccgga agtcgcaaac aatcagctcg
     5341 ggcggccggt cgtgcgcgcg caattgcgcg agcgcctcgt cgccgctggc cgccgcgagt
     5401 gtcaggcagc cccagcgttc gagcaggcta cacatggcag tgcgcgccgg ctcttcgtcg
     5461 tcgatcacac agacgaaggt gccgcgcaga tcagcccgca gcgccggctt gacggccggt
     5521 ggtggcacgg cggtcgcggt acccgagggc aggtcgaacc agaacaccga cccgcgtccc
     5581 agggtcgact gcagttcgac tcgggtgccg agcacttcga ccagccgcgc cacgatcgcc
     5641 aaacccaggc cgagccctcg ctgcggctgc gcaccagcgt gacccagccg gacgaactcg
     5701 cggaacactt cgtcgcgctg gtcctgcggg atgccacggc cggtgtccca tacggccagg
     5761 cggagcagac cggcgcgacg gcgcacgccg aacagtaccc ccccgcgctc ggtgcactgg
     5821 atggcgttgg ccaccagatt catgagaatg cgctggacga gaaccgggtc gctgcgcagc
     5881 caggcgtgcg ccgtgtgcac gcgaatctcg atcccctttg cgcgcgccag cggtgcgaat
     5941 tgcgcttcga tcggggtgag cacgctcgcc gcgtcgaaat cgatcggagc ggcctgcagc
     6001 agaccggcct cgagcctgga aaggtcgagc agctgatcga gcaggtcgga caggatcctg
     6061 gcagcgtctt ccacgcgctg gatgagcgct gcatgttcgt cggcgctgcg ggtcgtgcgc
     6121 agctgatcga cgaacagggc catggcgtgc atcggctggc gaagatcgtg gctggcaacg
     6181 gccaagaagc gcgtctgggc ccggttcgcc aagtcgagct ggcgcgtgcg ctccgccacc
     6241 cgccgttcga gttcggcgta ggtttggccc agccgctcgg ccatcgcgtt gaatcgatcg
     6301 gcgagctcct gcagttcatc gccgctcgcg atgtcgatgc gatgcgacag atcgccgctg
     6361 ccgaagcgct cggcgccgcg gcgcaactcg cccatcggtg cgaccatccg gcgcgccgcc
     6421 aagacgcccg ccgccaaggc catcgccagc gcgagcaaaa ggatcagtgc gcctcgcatg
     6481 accgacgcgg ccagcgggac aaatgcttcg gcacgaggct gctcgacgaa gacggtccac
     6541 gccaaggggg cgatgcttgc atgggcggca agcaccggca cgccgttctg gtcgcgtcca
     6601 gccgcatcga aaccctgccc ttgcacggcc agggccgcct ctacctgtgg aagccgcgac
     6661 aggtccgtca tctgcatcac gaagctgatg ttcgggtgcg ataccagccg accttgtttg
     6721 tcgacgacgt aggcgaatcc ggactggccg acctggatcc gtgagattac gttccataca
     6781 aacttgaggt tcacgtcggc caggatgacg ccgccgtcgc gtcctgcgct aggcaccgcc
     6841 atcgtgagat gcggctccgt ctgccccacg aattgcacgc tgctgaacgc cgagcgtcct
     6901 gcgcgcgcga cggtgaaatc cgagtcgttg gaccagtcga ccccgctggc cacgcgatcc
     6961 ggttcgatgc gagacacatg cacctgctcg attcccgaac gatcgaccag cgttgcagcg
     7021 gtgacggccg gctcgagccg aagcagcttc aggagttcga gccgccgcag ggttagctca
     7081 ttgccggccc ctttggccgc acccagggtc gcccaatcca ggctccgccg caggtcgtcg
     7141 aatacacgtg atacggcctg cgcggctgac tgagcctttt ccagctgcag ggcttcaagc
     7201 acggccaggc gctcgcggtg tgccgtccag atctccactg cgccgccgat cgtcagcgca
     7261 ctgcacagca acgcgacgac gagcgccgca tatcgggtgg cgatcgagcg accggtcatt
     7321 gagcgcaatc tacgcgcagt gtcccaacat tcaacgggga cttcggtcct gtgtccttgc
     7381 gagcgtgatc ggtcggcgct tcagaccagg accatggtcc tgagaccgaa gtcgcgtcgc
     7441 atcgcgcgca cggggtacct cggtcggatg gtcagggcct tcgttgcgag acagactgcg
     7501 acgccatagg tcaccccagg agcacgagca tgagcacgac gacaaaggct cttacgttcg
     7561 cggcgctggc cgcactggcg ctgagcgcat gggcccagca ggggactctt gacaagatca
     7621 aggcctcggg cgccatcacg atcggccatc ccaacgtgat ccccttcgcc taccgcgcgg
     7681 acgggccgca gcccgttggc ttcgcgatcg atctatgcgc gcgcgttgtc gacgcgatca
     7741 aggccaagct gaagctgcct gagctcaagg tcaactatta ccgggagacg acgaccccgg
     7801 ccacgcgtat cccgttgctg gtcgacggta ctatcgacct ggactgcggc gcgagcacca
     7861 acaccgccga gcgccagaag caggtcgcct tctcgatgac gtacttcctg agcgtgacgc
     7921 gcttcatcgc aaagaaggac gacaacctga ccaagctcgc agacctcaag ggcaagacgg
     7981 ttgcctcgca attgagcacg cccgccctca agcaaatcac cgagctgaat gcggcgcaga
     8041 acctgggcct caacatcctg gtcgtcaact atgggcgtga agtgatgccg acactggaaa
     8101 gcggccgcgc cgtcgccttc gtgtacgacg acgtcctgct ggcgggattc gcggccaatc
     8161 acgccgatcc aggtctgtgg cagatctcga acgaagcgct gtcgctgccc gaaccctacg
     8221 ggatcatgtt gcgaaaggat gatccagaat tcaagaaggc ggtcgatgcg gcgatggtcg
     8281 ccacctacaa gagcggtgag gccgccaggc tctacgcc
//
LOCUS       DBNHBY010000575         5495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_647210, whole genome shotgun sequence.
ACCESSION   DBNHBY010000575 DBNHBY010000000
VERSION     DBNHBY010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_647210"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="AC16HB_14015"
     CDS             complement(<1..879)
                     /locus_tag="AC16HB_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5607950.1"
                     /translation="MGEFELWYLILLPALFAAGWWLRGVDTRARGTDPQTTPRSMFRG
                     LNYFLNEQPDKAIDAFIEVVKLDSETIELHHALGNLFRRRGEIDRAVRIHTHLLNRAD
                     LPAAERANALAELGQDFLKGGLLDRAEEAFLKLRDEPRHRLDALRSLARIYEMEREWV
                     KAIDCARRLEREAGESHHAAIAHFNCELADQALARGDLEAARGFLAEAQAADRKSVRA
                     AILAGELASRRGDLEQAIALWSQIEATSPEHLALVARRLTDALDKVGRRGEALNLLRR
                     GLMDAPSIDLLDAAVER"
     gene            complement(886..1239)
                     /locus_tag="AC16HB_14020"
     CDS             complement(886..1239)
                     /locus_tag="AC16HB_14020"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR5607951.1"
                     /translation="MNALAWLIRIAFFLVVLWFAFKNLTPVSVHLSETLHWDSVPLII
                     VMLSCLLIGVLAGAIAMAPRVFRLRRQLAAAEARVAQAPPTIRRGPETITGMANVARN
                     AGAVGELNGDTRIGS"
     gene            complement(1354..1674)
                     /locus_tag="AC16HB_14025"
     CDS             complement(1354..1674)
                     /locus_tag="AC16HB_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR5607952.1"
                     /translation="MTKSELIAKLAERNPRLVARDADEAVKTMLDAMSDALAQGSRIE
                     IRGFGSFALNYRPPRIGRNPKSGERVHVPAKHVPHFKAGKELRERVDGAPRDGDAAAP
                     SSGD"
     gene            complement(1725..3428)
                     /gene="rpsA"
                     /locus_tag="AC16HB_14030"
     CDS             complement(1725..3428)
                     /gene="rpsA"
                     /locus_tag="AC16HB_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5607953.1"
                     /translation="MSSVATQSAPAKESFAALFEESLSRKEMRAGEVITAEVIRVDYN
                     FVVVNAGLKSEAYIPIDEFKNDLGQIDVKPGDFVPVAIESLENGYGDTILSRDKAKRL
                     AAWMNLERALDGGDLVSGTITGKVKGGLTVMTNGIRAFLPGSLVDVRPMKDTSGLEGK
                     TLEFKVIKLDRKRNNVVLSRRAVVEASMGEERQKLLETLKEGAIVKGVVKNITDYGAF
                     VDLGGIDGLLHITDLAWRRVRHPSEVVQVGQEITAKVLKFDQEKNRVSLGVKQLGEDP
                     WVGLGRRYPQGTRLFGKITNITDYGAFVEIEPGIEGLVHVSEMDWTNKNVDPKKVAQL
                     GDEVEVMVLEIDEDRRRISLGMKQCKPNPWEDFSLSHKKGDRVKGQIKSITDFGVFIG
                     LPGAIDGLVHLSDLSWNEPGEEAVRKYKKGDEVEAVVLAIDVERERISLGVKQLSGDP
                     FTNYAGTHEKGSIVTGTVKSVDPKGAVISLSGEVEGYLRASEIASDRVEDARNHLREG
                     DKVTALILNIDRKNRSINLSIKAKESAEATEAMARMQAETASGTTNLGALLKAKLDKT
                     T"
     gene            complement(3525..4202)
                     /gene="cmk"
                     /locus_tag="AC16HB_14035"
     CDS             complement(3525..4202)
                     /gene="cmk"
                     /locus_tag="AC16HB_14035"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR5607954.1"
                     /translation="MPIAEFAVPVIAIDGPTASGKGTVAAAVARALGFHYLDSGALYR
                     LVGLAARDANVSLDDGAALAGLAATLRPRFAGGRIALDGRDLTDAIRTEEASQWSSRV
                     AVHAPVRQALLDLQRAFRKPPGLVADGRDMGTVVFPYAVLKVFLTASVEARAERRYKQ
                     LIEKGFPAKLSSLLQDLRERDERDTKRAAARLKPAEDAYQLDSSWLSIEEVVAQILHW
                     YAMTAAR"
     gene            4098..4394
                     /locus_tag="AC16HB_14040"
                     /pseudo
     CDS             4098..4394
                     /locus_tag="AC16HB_14040"
                     /inference="COORDINATES: protein motif:HMM:NF021362.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
     gene            complement(4333..>5495)
                     /gene="aroA"
                     /locus_tag="AC16HB_14045"
     CDS             complement(4333..>5495)
                     /gene="aroA"
                     /locus_tag="AC16HB_14045"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR5607955.1"
                     /translation="ADLFLGNAGTAMRPLTAALAFAGGRYRLDGVARMRERPIGDLVD
                     ALNTLGAHIGYAGRPGYPPLVIAPADPLTRDTVDIKGEVSSQFVSGLLMAAPSIAPPG
                     GLTIRVPGTLISQPYVAMTVAMMAQFGVTVARAGSSFQVPPARYATPGRYAVEGDASG
                     ASYFLALGAIAGGPVRVLGVGRGSVQGDVAFADLLQKMGAEITWGVDFIESRAGPAVH
                     AIDLDCTEIPDAAMTAAVVALFARDRSVLRGIGSWRVKETDRIAAMATELRKLGAKVE
                     EGPDWIAIDPPTILREATVDTYDDHRIAMCFALAAAAGVPVHIRDPRCVAKTFPDYFK
                     RLAGLVQPAPCREPRHAHAGSMRRCWQAAPAARSRRRSARCRSARTRPRTRP"
BASE COUNT          855 a         1917 c         1777 g          946 t
ORIGIN      
        1 gcgctccacc gcggcgtcga gcaggtcgat cgacggcgca tccatcaacc cgcgccgcag
       61 caggttcagt gcttcgccgc ggcggccgac cttgtcgagc gcgtcggtca ggcggcgcgc
      121 gaccagcgcg aggtgttcgg gggacgtggc ctcgatctgc gaccacagcg cgatggcctg
      181 ctcgaggtcg ccgcggcgcg atgcgagttc accggcgagg atcgcggccc gcaccgactt
      241 gcggtcggcg gcctgcgcct cggccaggaa tccccgcgcg gcctcgaggt cgccgcgcgc
      301 gagcgcctga tcggcaagct cgcagttgaa atgcgcgatg gcggcgtgat gcgattcgcc
      361 cgcctcgcgc tccagccggc gcgcgcaatc gatcgccttg acccactcgc gttccatctc
      421 gtagatgcgc gccagcgagc gcagcgcgtc gagccggtgg cgcggctcgt cgcgtagctt
      481 caggaatgcc tcctcggcgc ggtcgagcaa cccgcccttc aggaagtcct gtcccagttc
      541 cgccagcgcg ttggcgcgct cggccgcggg aaggtcggcg cggttgagaa ggtgtgtgtg
      601 gatgcgcacc gcgcgatcga tctcgccgcg acggcggaac aggttgccga gcgcatggtg
      661 cagctcgatc gtctcggagt cgagcttgac cacctcgatg aacgcgtcga tcgccttgtc
      721 cggctgctcg ttcaggaagt agttcaaacc gcgaaacatc gagcgcggcg tcgtctgcgg
      781 gtccgtgccc cgcgcgcgcg tgtcgacccc gcgcaaccac cacccggccg cgaacagcgc
      841 cggcagcagg atcaggtacc agagttcgaa ttcgcccacg aagggtcagc tgccgatgcg
      901 cgtatcgccg ttcagctcgc cgaccgcgcc ggcgttgcgg gcgacgttgg ccatgccggt
      961 gatcgtctcc gggccgcgcc ggatcgtcgg cggcgcctgg gcgactctgg cctcggccgc
     1021 agcgagctga cggcgcagtc ggaacacccg cggcgccatc gcgatcgcgc cggccagcac
     1081 gccgatcagt aggcacgaca gcatgacgat gatcagcggc acgctgtccc agtgcagcgt
     1141 ttcggacaga tgcaccgaca ccggcgtcag gttcttgaag gcgaaccaca gcacgacgag
     1201 gaagaaggcg atccggatca accacgcgag agcgttcatc ggggcatcgg tgctggaacc
     1261 gcgacgattg tacggtgcag gcgcgggcgc tcgatcgacg ctgggggcga aaaaaaggcc
     1321 ccgcagtgcg gggccgtttg ccggttctcg gtgctagtcg ccgctcgaag gagcggccgc
     1381 gtcgccgtcg cgcggcgcgc cgtcgacccg ctcgcgcagt tccttgccag ccttgaagtg
     1441 cggcacgtgc ttggcgggca cgtgcacgcg ctcgcccgac tttggattgc gcccgatgcg
     1501 cggcggacgg taattcagcg caaagctgcc gaaaccgcgg atctcgatcc ggctgccttg
     1561 cgccagcgcg tcgctcatcg cgtcgagcat cgtcttgacg gcttcatcgg cgtcgcgcgc
     1621 caccaggcgc ggattgcgct cggcgagctt cgcgatcagc tccgacttcg tcatcatttt
     1681 catgggtgga tcgcgtcggt gcgctggcgc gcaccgacga ctcctcaggt tgtcttgtcc
     1741 agcttcgcct tcagcagcgc gccgaggttg gtggtgccgc tggcggtctc ggcctgcatc
     1801 cgcgccatcg cctcggtcgc ctcggcgctt tccttcgcct tgatcgacag gttgatgctg
     1861 cggttcttgc ggtcgatgtt caggatcagc gcggtcacct tgtcgccttc ccgcaggtga
     1921 ttgcgcgcgt cctcgacgcg gtcgctggcg atctccgagg cacgcaggta accctcgacc
     1981 tcgccgctga gcgagatcac cgcgcccttc ggatcgacgc tcttcaccgt gccggtgacg
     2041 atcgaaccct tctcgtgcgt gccggcatag ttggtgaacg ggtcgcccga cagctgcttg
     2101 acaccgagcg aaatgcgctc gcgctccacg tcgatcgcca gcaccacggc ctcgacctcg
     2161 tcgcccttct tgtacttgcg caccgcctct tcgccgggct cgttccacga caggtcggac
     2221 aggtgcacca ggccgtcgat ggcgcccggc agtccgatga agacgccgaa gtccgtgatc
     2281 gacttgatct gacctttgac gcggtcgccc ttcttgtggg acagggaaaa gtcctcccac
     2341 gggttcggct tgcactgctt catgccgagc gagatgcggc ggcggtcctc gtcgatctcg
     2401 agcaccatca cctcgacctc gtcgccgagc tgtgccactt tcttcgggtc gacgttcttg
     2461 ttggtccagt ccatctcgga cacgtgcacc aggccttcga tgccgggctc gatctcgacg
     2521 aacgcgccgt agtcggtgat gttggtgatc ttgccgaaca ggcgcgtccc ctgcgggtag
     2581 cgccgcccca gcccgaccca cggatcctcg ccgagctgct tcacgcccag cgaaacgcgg
     2641 ttcttctcct ggtcgaactt caggaccttg gccgtgatct cctgaccgac ctgcaccacc
     2701 tcgctcggat ggcgcacgcg acgccacgcg agatccgtga tgtgcagcag gccgtcgatt
     2761 ccgcccaggt cgacgaacgc gccgtagtcg gtgatgttct tgacgacgcc cttgacgatc
     2821 gcgccttcct tgagcgtctc gagcagcttc tgccgctcct cgcccatgct cgcctcgacc
     2881 acggcgcggc gcgacagcac cacgttgttg cgcttgcggt cgagcttgat gaccttgaac
     2941 tcgagcgtct tgccctccag gccggaggtg tccttcatcg gccgcacgtc gaccagcgaa
     3001 cccggcagga acgcgcggat gccgttggtc atgaccgtca ggccaccctt gaccttgccg
     3061 gtgatcgttc cggacacgag gtcgccgccg tcgagcgcgc gctcgaggtt catccatgcg
     3121 gccagccgct tggccttgtc gcgcgacaag atcgtgtcgc catagccgtt ttccagagat
     3181 tcgattgcca ccgggacgaa gtcgcccggc ttcacgtcga tctggccgag gtcgttcttg
     3241 aactcgtcga tcgggatgta tgcctcggac ttcagccccg cgttgacgac cacgaagttg
     3301 tagtcgacgc ggatcacctc ggcggtgatg acttcgcctg cgcgcatttc cttgcgcgac
     3361 aggctttctt cgaacagtgc ggcaaagctt tcctttgccg gagcagattg agttgcaacg
     3421 gaagacatgc aatgactttc aatcacgccc aggttttgcg cccgagcggt gttgaacacc
     3481 acgcgccgga ggaccgcgga aaccggcgcg cacccacggc cgcgctacct tgcggccgtc
     3541 atcgcgtacc agtgcaggat ctgcgctacg acctcctcga tcgacagcca cgaagagtcc
     3601 agctggtacg catcttctgc cggtttcagc cgcgccgcgg cgcgtttggt atcgcgctcg
     3661 tcgcgttccc gcagatcctg cagaaggctg gaaagtttag ctggaaagcc cttttctatc
     3721 aactgcttat agcgccgttc ggcacgcgct tcgacgctag cggtcagaaa gaccttgagc
     3781 accgcataag gaaacacgac tgtgcccatg tcgcggccgt cggccaccag ccccggcggc
     3841 ttccggaacg cgcgctgcag gtcgagcagc gcctgccgta ccggcgcgtg caccgcgacc
     3901 cgcgacgacc actggctcgc ttcctcggtg cggatcgcgt cggtcaagtc gcgaccgtcg
     3961 agcgcgatgc gcccgcccgc aaaccgcggg cgcagcgtcg ccgccaatcc cgccagcgct
     4021 gcgccatcgt cgagcgacac gttcgcatcg cgcgcggcga ggccgaccag ccggtacagc
     4081 gccccgctgt cgaggtagtg aaaaccgagc gcacgcgcca ccgcggcggc aaccgtgccc
     4141 ttgcccgacg cggtcgggcc gtcgatggcg atgactggaa ccgcgaattc ggctatcggc
     4201 atgatcggaa cattcaactg gacgcgcgca gtcaggcggc tagtctgtca gccatcgacg
     4261 gatcggagcc cgccatgcga attccactga gactgagcct cgccaaaacc ggcacgtttg
     4321 ccctggtcca cttcacggtc gcgttcgggg tcgcgtacgc gctgaccggc agcgcgccga
     4381 tcgccggcgc gatcgcgctg ctggagccgc ttgccaacac cgtcgcatac ttcctgcatg
     4441 agcgtgcctg ggctcgcggc acggcgccgg ctgcaccagc cccgcaagac gcttgaagta
     4501 gtcggggaag gtcttggcca cgcagcgcgg gtcacggata tggaccggca ctcccgccgc
     4561 ggcggcgagc gcaaagcaca tcgcgatgcg gtgatcgtcg taggtgtcga ccgtcgcctc
     4621 gcgcaggatc gtgggcggat cgatcgcgat ccagtcgggc ccctcttcca cttttgcgcc
     4681 gagcttgcgc agttcggtgg ccatcgcggc gatgcgatcg gtctccttga cgcgccatga
     4741 accgatccca cgcaatacgc tgcggtcgcg cgcgaacagc gcaacgaccg ccgcagtcat
     4801 cgcggcgtcg ggaatttcgg tgcaatcgag gtcgatcgcg tggaccgccg gtccggcgcg
     4861 tgactcgatg aagtcgacgc cccacgtgat ctccgcgccc atcttctgca gcagatcggc
     4921 gaacgccaca tcgccctgca cgctgccgcg gccgacgccg agcacgcgca ccggcccgcc
     4981 ggcgatcgcg ccgagcgcca ggaaatacga ggcgcccgat gcatcgccct cgaccgcata
     5041 gcggccgggc gtcgcatatc gcgcgggcgg cacttgaaag ctgctgcctg cacgcgccac
     5101 cgtcacgccg aactgcgcca tcatcgcgac cgtcatcgca acatagggct gcgagatcaa
     5161 ggtgcccgga acgcggatcg tcagccctcc gggcggtgca atcgacggcg cggccatcag
     5221 caatccgctg acgaactggc tcgacacctc gcccttgatg tcgaccgtat cgcgcgtcaa
     5281 cgggtcggca ggcgcgatca ccagtggtgg atagccgggg cgccctgcgt agccgatgtg
     5341 cgccccgagc gtgttcagcg cgtcgaccag atcgccgatg ggccgctcgc gcatccgcgc
     5401 cacgccgtcg agccgatagc ggccaccggc aaacgcgagt gctgccgtca gcgggcgcat
     5461 tgcggtgccg gcgttaccca gaaagagatc cgcct
//
LOCUS       DBNHBY010000576         3007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1188391, whole genome shotgun sequence.
ACCESSION   DBNHBY010000576 DBNHBY010000000
VERSION     DBNHBY010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1188391"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="AC16HB_14050"
     CDS             complement(<1..760)
                     /locus_tag="AC16HB_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR5607956.1"
                     /translation="MEFLALEFWYALAAIVVIDLVLAGDNAIVIALAARNVPKHLQKQ
                     AVVWGAVGAIAVRSSMTILVVWLLRVPGLLAAGGAMLVWIAYRLLLPEEENGNGHGVG
                     GADSFWGAIKTIVVADMVMGLDNVLAVAGAAQGSYLLVVLGLLISVPMVIWGSTMLLR
                     FVERFPAFVYLGAGVLAFTAVKMLTSEPLLKDAFTAHKLTVPLLYAVTVFGVLWAGFV
                     RNHRRLQSRISARLAHFARLRETAAAAPAADPDKG"
     gene            871..1782
                     /gene="nhaR"
                     /locus_tag="AC16HB_14055"
     CDS             871..1782
                     /gene="nhaR"
                     /locus_tag="AC16HB_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HIR5607957.1"
                     /translation="MSALNLKHLRYFWEVARSGGIARASERLHVTPQTISGQLSLFER
                     SLGYRLFTRSGRRLELTDSGRLALNYADEIFTLGDELEDVLRRPASGRARLFRVGVAD
                     AVAKDVAYRLMEPAMRLPEPMRIVCREGTLASLLAELSVRRLDIVIADSPLPASVNVR
                     GFSHLLGQCGITFFATPKLAQVLKGPFPRLLDGAPLLLPGEQAAVRPRLMRWFDGQQI
                     RPRIVGEFDDSALLKAFGRAGAGVFAAPTAIADEVRKQYGAVAVGRTDAVSEAFYAIS
                     VERRLTQPAVVAITSAARRDLFRNRRR"
     gene            complement(1795..2079)
                     /locus_tag="AC16HB_14060"
     CDS             complement(1795..2079)
                     /locus_tag="AC16HB_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607958.1"
                     /translation="MQYLISPRAGRAFIGAHPCRLPQPAAADRPSNELPRRRGEALRN
                     FFPKLAAWLEDSAHRARRREVEDYLSQAKDISDLEERIRRVERQQASQLF"
     gene            complement(2291..>3007)
                     /locus_tag="AC16HB_14065"
     CDS             complement(2291..>3007)
                     /locus_tag="AC16HB_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5607959.1"
                     /translation="VALTESALAVLIGRSPKEVFGPSIARGAGIAAVVAPEVPAGLPS
                     DLLARRPDIRQAEAQLSAADARIAEARAQYYPALTLTARFGAESAELSDLLTAPARVW
                     SIGAGLLQPILGANTIRAQVDAATARREQAAIGYQQAVQAAFRDVHDALAAHGSARDA
                     YVAQDERRARVTEALRLAELRQKSGYSSYLDVLDAQRNLLDAERVRLAALRDRQTAIV
                     DLFKALGGGWDPAAPTTAQR"
BASE COUNT          483 a         1048 c         1019 g          457 t
ORIGIN      
        1 ctcctttgtc ggggtccgcg gccggcgcgg cggcagcggt ttcgcgcaac cgggcaaagt
       61 gcgccagacg cgcgctgata cgtgactgca gccggcgatg gttcctgacg aagccggccc
      121 acagcacgcc gaacaccgtg accgcgtaca gcagcggaac ggtcagcttg tgggccgtga
      181 aagcgtcctt cagcaggggc tcggacgtca acatcttcac cgcggtgaac gcgagcacgc
      241 cggcgcccag atagacgaaa gcaggaaacc gttcgacgaa ccgcagcagc atggtgctgc
      301 cccagatcac catcggcacg ctgatcagca ggccgagcac gaccagcagg tagctgccct
      361 gcgccgcgcc cgccaccgcg agcacgttgt ccagccccat caccatgtcg gcgacgacga
      421 tggtcttgat cgcgccccag aaggagtcgg cgccgccgac gccgtggccg ttgccgttct
      481 cttcctcggg cagcagcagt ctgtaggcga tccacaccag cattgcaccg ccggcggcga
      541 gcaggcccgg gacgcgcagc agccacacca ccagtatcgt catcgagctg cgcaccgcga
      601 tcgcgccgac cgccccccac acgaccgcct gcttctgcaa gtgcttgggc acgttgcgcg
      661 ccgccagcgc gatcacgatc gcgttgtcgc ccgccagcac gagatcgatg acgacgatcg
      721 ccgccagcgc gtaccagaac tccagcgcca ggaactccac ccgattgcct cgcctgcttc
      781 tcgttgtcga tgcgtagcgc cgaacttaac gcggccgtca ccgttcggca attcgaatat
      841 cctgtggact tcgttcggaa aaaccgaacc atgtccgcgc tgaacctcaa gcacctgcgc
      901 tacttctggg aggtcgcccg ctccggcggc atcgcgcggg ccagcgagcg actgcacgtg
      961 acgccgcaga cgatcagcgg acagctgagc ctgttcgagc gctcgctcgg ctatcgcctg
     1021 tttacgcgca gcgggcgccg gctcgaactg accgactccg gccggctcgc gctgaactat
     1081 gcggacgaga tcttcactct cggcgatgaa ctcgaggacg tgctgcgccg acccgcgagc
     1141 gggcgggcgc gactgttccg ggtcggcgtc gccgacgcgg tcgccaagga cgtcgcctac
     1201 cggctgatgg aaccggcgat gcgcctgccg gagccgatgc gcatcgtgtg ccgcgaaggc
     1261 acgctcgcca gcctgctggc ggaactgtcg gtgcgccggc tcgacatcgt gatcgccgac
     1321 agcccgctgc ccgcgtccgt caacgtgcgc ggcttcagcc acctgctcgg ccagtgcggg
     1381 atcacgttct tcgcgacacc gaagctggcg caggttctca agggtccgtt tccgcgcctg
     1441 ctcgacggcg cgccgctgct gctgcccggc gagcaggcgg cggtgcggcc gcggctgatg
     1501 cgctggttcg atgggcagca gatccgtccg cgtatcgtcg gcgagttcga cgacagcgcg
     1561 ctgctcaagg cgttcggccg cgccggggcg ggcgtctttg cggcaccgac ggcgatcgcg
     1621 gacgaggtgc gcaaacagta cggtgcggtc gcggtcggcc gcaccgacgc ggtcagcgaa
     1681 gccttttatg cgatctcggt cgaacgccgg ctgacgcagc cggcggtggt cgcgatcacg
     1741 tcggcggcgc gcagggatct tttccgcaac cgccgccgct gacgcgcaaa cgtttcagaa
     1801 gagttgcgag gcctgctggc gctcgacgcg acgaatccgt tcttccaggt cggaaatgtc
     1861 cttggcctgc gacaggtaat cctcgacctc gcggcgccgc gcgcgatgcg cggaatcctc
     1921 cagccacgcc gccagtttcg ggaagaagtt gcgcagcgcc tcaccgcggc gcctcggaag
     1981 ttcattcgaa ggccggtcgg cggcggccgg ctgaggcaag cggcaggggt gcgcgccgat
     2041 gaaggcccgg cccgctcggg gcgaaatcag gtactgcatg acatgctcct gtgagccgca
     2101 acatcacgtt gtgatgctgc atagcgacaa tcacagtatc ggtgagaaat cacagaataa
     2161 aagtcggctt ttcgatagcg attgctcact cctattgcat accgcgtcga atttgacgca
     2221 ctgcagtagt agagttcctc agatcctgag ccgcgttggc gcggatgcaa caggcgccgt
     2281 ggaacgccgg tcagcgttgc gcggtggtcg gcgcagccgg atcccagccg ccgccgagtg
     2341 ccttgaacag atcgacgatc gccgtctggc ggtcgcgcag cgcggcgagc cgcacacgct
     2401 ccgcatcgag caggttgcgc tgcgcgtcga gcacgtcgag gtagctcgag tagccgcttt
     2461 tctgccgcaa ctcggcgagc cgcagcgcct cggtgacacg cgcgcggcgc tcgtcctgcg
     2521 cgacgtacgc gtcgcgcgcg ctgccgtgcg cggccagtgc atcgtgcacg tcgcggaacg
     2581 ccgcctgcac cgcctgctgg tagccgatcg cagcctgctc gcggcgcgcg gtcgcggcgt
     2641 cgacctgcgc ccggatcgtg ttcgcgccga ggatcggctg caacaggccg gcgccgatcg
     2701 accacacgcg ggcgggagcc gtcagcaggt ccgacagctc cgcgctctcg gcgccgaagc
     2761 gcgcggtcag cgtcagcgcg gggtagtact gcgcgcgcgc ctcggcaatg cgcgcatcgg
     2821 cggcggaaag ctgcgcctcg gcctggcgga tgtcgggacg ccgcgcgagc aggtcggacg
     2881 gcagcccggc cggcacttcc ggcgccacga cggcggcgat cccggcgccg cgggcgatcg
     2941 acgggccgaa cacttccttc gggctgcggc cgatgagtac cgcgagcgcg gactccgtca
     3001 atgcgac
//
LOCUS       DBNHBY010000577         3853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1797265, whole genome shotgun sequence.
ACCESSION   DBNHBY010000577 DBNHBY010000000
VERSION     DBNHBY010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1797265"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /gene="dnaE"
                     /locus_tag="AC16HB_14070"
     CDS             complement(<1..1819)
                     /gene="dnaE"
                     /locus_tag="AC16HB_14070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5607960.1"
                     /translation="MARMIRMSAPFVHLRLHSEFSVVDGLVRIDDAVKAALADGQGAL
                     ALTDSTNLFGAVRFYKAARAGGVKPILGCDLWIANDADRDHPHRLLVLVQDHAGYLNL
                     CELLSRAFLENQHRNRAELRSEWLGGDATCGLIALSGAAEGEVGAQLLAGNRAAARAA
                     AQRLAAAFPNRFYLELQRAGRSGDDACVSATLALALELRLPVVATHPVQFVAADEFRA
                     HEARVCIAEGYTLADPRRPRRFTAQQYFKSAAEMADLFKDVPAALANSVEIARRCNLQ
                     LTLGKPRLPDFPTPPGVTLDEHCRDLALAGLEKRLLKLFSDDSERDRRRPTYVERLEW
                     ELATIAKMGFSGYFLIVADFINWAKENGIPVGPGRGSGAGSLVAYALNITDLDPLRYE
                     LLFERFLNPERVSMPDFDIDFCQDNRDRVIDYVKGKYGAQAVSQIATFGTLGAKAVVR
                     DVGRVLDLPYTKCDTLSKLIPHNPADPWTLERALAEEPAFAEAVRADEEYAELVELSK
                     PLEGLTRNVGMHAGGVLIAPGKLIDFCPLYCAQGTDAVISQFDKDDVEAIGLVKFDFL
                     GLTTLTILDLTLRFVKQLDPASTITLEDLPLDDAKTYEVF"
     gene            1920..2399
                     /locus_tag="AC16HB_14075"
     CDS             1920..2399
                     /locus_tag="AC16HB_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607961.1"
                     /translation="MPRTTCSMLLAALLAGLVAGSAGADTIYKVRLKDGSIAFTDRPP
                     PGSMILDKREAQPPPAVLPAVTPKPDRASTDGRVPVPDSERKEAAVAAAERALAKAKE
                     DLEQGRNPREGDFIGTARKGFVRPSPAFEERVRALEQAVADAQARLAKAIEARDAAR"
     gene            2412..2981
                     /locus_tag="AC16HB_14080"
     CDS             2412..2981
                     /locus_tag="AC16HB_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR5607962.1"
                     /translation="MIGARTAPVRRRWIDRALHVLATTTSSRWYAPLAGAIAFALTLT
                     ASVPYTAILIAALWLTPQRWKSLVLFSSLCGALGAIVLLAGFRHLGWAPLQAAYPELA
                     AAPAWQRVVQAVQDYGVAALGVVAAAPLPQTPALAIAASAKLDPAAVFAALFVGKVVK
                     YSVVSWLVSIGSPLVDRVVRRARLPFTRR"
     gene            3042..>3853
                     /gene="glgP"
                     /locus_tag="AC16HB_14085"
     CDS             3042..>3853
                     /gene="glgP"
                     /locus_tag="AC16HB_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5607963.1"
                     /translation="MDRPGTRFSLEIRPGLPPRLARLAEIAGDLYYSWDLSARGLFAR
                     LDPGLWDTCGHSPKSFLNQVDQRRLEAAAEDPGYLSHFNRVLSTYDTYLARTSPHPNG
                     DWLGEGDLVAYFCAEFGFHESLPIYSGGLGILAGDHCKAAADCGLSFVGVGLLYRQGY
                     FSQEIDFDGNQRATYHDADFDDLPVAPAHTGDGAELHIPIEVGERIVQVKVWLSRVGR
                     VRLILLDTDLPENSDHDRFIAHRLYGGDRGTRIEQEIVLGMGGVRALAALNLR"
BASE COUNT          610 a         1299 c         1315 g          629 t
ORIGIN      
        1 ggaacacctc gtaggtcttc gcgtcgtcga gcggcaggtc ttccaacgtg atcgtcgacg
       61 ccgggtcgag ctgcttcacg aagcgcagcg tgaggtccag gatcgtcaat gtcgtcagcc
      121 cgaggaagtc gaacttcacc aggccgatcg cctcgacgtc gtccttgtcg aactggctga
      181 tgacggcgtc ggtgccctgc gcgcagtaca gcgggcagaa gtcgatgagc ttgcccggcg
      241 cgatcagcac gccgcccgcg tgcatgccga cgttgcgagt cagcccctcg agcggcttcg
      301 acagttccac cagctccgcg tattcctcat cggccctcac cgcttcggcg aacgcgggct
      361 cctctgcgag cgcacgctcg agcgtccacg ggtcggcggg gttgtgcgga atcagcttcg
      421 acagcgtgtc gcacttggtg tagggcaggt cgagcacgcg gccgacgtcg cgcaccaccg
      481 ccttggcgcc gagcgtgcca aaggtcgcga tctggctcac cgcctgcgcg ccgtacttgc
      541 ccttcacgta gtcgatcacg cggtcgcggt tgtcctggca gaagtcgatg tcgaagtccg
      601 gcatcgacac gcgctcggga ttgaggaaac gctcgaacag cagttcgtat cgcagcgggt
      661 ccagatcggt gatgttcaac gcatacgcga ccagtgaccc cgcacccgag ccccgcccgg
      721 gaccgaccgg gatgccgttt tccttggccc agttgatgaa gtcggcgacg atcaggaagt
      781 agccggagaa gcccatcttc gcgatcgtcg cgagttccca ttccaggcgc tcgacgtagg
      841 tcgggcgtcg gcggtcgcgc tccgagtcgt cggaaaagag cttcagcaag cgcttctcga
      901 gcccggccag cgcgagatcg cggcaatgct cgtcgagcgt gacgccgggc ggcgtcggga
      961 agtcgggcag ccgcggcttg ccgagcgtca gctgcaggtt gcagcgacgc gcaatctcga
     1021 ccgagttggc aagcgccgcc gggacatcct tgaacaggtc tgccatttcc gccgcggact
     1081 tgaagtactg ctgcgcggtg aaacggcgcg gccggcgcgg gtcggccagc gtgtagccct
     1141 cggcgatgca cacgcgcgcc tcgtgggcgc ggaattcgtc cgccgcgacg aattgcaccg
     1201 gatgcgtggc gacgaccggg agccgcagtt cgagcgccag cgcgagcgtc gcgctaacgc
     1261 atgcatcgtc accgctgcga cccgcgcgct gcagttcgag atagaaccgg ttcgggaacg
     1321 cggcagcaag tcgctgcgcg gccgcgcgcg cagcggcgcg attccctgcg agcagctgtg
     1381 cgccgacctc gccctcggca gcgcccgaca gcgcgatcaa cccgcacgtc gcgtcgcccc
     1441 cgagccactc ggagcgcagc tccgcccggt tgcgatgctg gttctccagg aacgcgcgcg
     1501 aaagcagttc gcacaggttg agatatccgg cgtggtcttg caccagcacg agcagccggt
     1561 gcggatgatc gcgatccgcg tcgttggcga tccagaggtc gcagcccagg atcggcttca
     1621 cgcccccggc gcgcgcggcc ttgtagaagc gcaccgcgcc gaacaaattg gtgctgtcgg
     1681 tcagcgcgag cgcgccctgg ccgtcggcca gggcggcctt cactgcatcg tcgatccgca
     1741 ccagcccatc gacgaccgag aactccgagt gcagtcgcag gtgaacgaac ggcgcggaca
     1801 tgcggatcat tctagcgatc cgcgctgact cgatccgtgt taagcgcttc gtcgcacttg
     1861 ctttcgttgc cgccggcggt tacgttgctg attgaatgat ccgcaatcga aggccgccga
     1921 tgccgcgtac cacctgctcg atgctgctcg cagcgttgct cgccggtctc gtggcgggca
     1981 gcgcgggcgc cgacacgatc tacaaggtca ggctgaagga cggctcgatc gcattcaccg
     2041 accggccgcc gcccggctcc atgatcctgg acaagcgcga agcccaaccg ccgccggcgg
     2101 tgctgcccgc agtgacgccg aagccggacc gtgcaagcac cgacgggcgc gtgcctgtgc
     2161 ccgacagcga acgaaaggaa gccgcggtcg ccgccgcgga gcgtgcgctg gccaaggcga
     2221 aggaggacct ggagcagggg cgcaatccgc gcgagggcga tttcatcggc acggcacgca
     2281 agggattcgt gcgcccatca cccgcattcg aggagcgcgt gcgcgcgctc gagcaagcgg
     2341 tggctgatgc acaggcgcgc ctcgccaagg caatcgaagc acgcgatgcc gcgcgctagg
     2401 cgcagcgaca catgatcggc gcgcgcactg cgccggtgcg gcggcgctgg atcgatcgcg
     2461 cgctgcacgt gctggcaacg accaccagca gccgctggta cgcgccgctc gcgggcgcga
     2521 tcgcgttcgc gctgacgctg actgcgagtg tgccgtacac ggcgatcctg atcgccgcgc
     2581 tgtggctgac tccgcagcgc tggaagtcgc tcgtgttgtt ctcctcgctc tgcggcgcgc
     2641 tgggcgccat cgtgctgctg gccggtttcc ggcacctcgg ctgggcaccg ttgcaggcgg
     2701 cctatcccga gctggctgcc gcgccggcct ggcagcgtgt agtgcaggcg gtgcaagact
     2761 acggcgtggc cgcgctcggt gtcgtcgcgg cggcaccatt gccgcagacg cctgcgctcg
     2821 cgatcgcggc aagcgcgaag ctcgatcccg ccgccgtgtt cgcggcgctg ttcgtcggca
     2881 aggtcgtgaa gtacagcgtg gtgagctggc tggtgtcgat cgggagccca ctggtcgatc
     2941 gcgtcgtcag acgggcgcgg ctgcccttca cgcgccgctg acacgcgcgc cgattcggta
     3001 agctagcagc aaaccacggc gcgctgatcg cgagaagacc aatggatcga cccggcaccc
     3061 gtttcagcct cgaaatccga cccgggttgc cgccgcggct ggcgcggctt gcggagatcg
     3121 caggcgacct ctactacagc tgggatctgt ccgcgcgcgg cctgttcgcc cgcctcgatc
     3181 cggggctgtg ggacacctgc ggccacagtc ccaagtcgtt tctgaaccag gtcgatcagc
     3241 gacggctcga ggccgcggcc gaagatcctg gttatctgtc gcatttcaac cgcgtactgt
     3301 cgacctacga cacgtacctt gcacgaacct ccccgcaccc gaacggcgac tggctgggcg
     3361 aaggcgatct ggtcgcctac ttctgcgcgg agttcgggtt tcacgagagc ctgccgatct
     3421 attcgggcgg gctcgggatt ctcgcgggcg atcactgcaa ggccgccgcc gattgcggcc
     3481 tgtcgttcgt cggcgtcgga ttgctgtacc ggcagggtta cttctcgcag gagatcgact
     3541 tcgacggcaa tcagcgcgcg acctaccacg atgcagactt cgacgacctg ccggtggcgc
     3601 cggcgcacac gggcgacggg gccgaactgc acattccgat tgaggtcggc gagcgtatcg
     3661 tgcaggtcaa ggtctggctg tcgcgcgtcg ggcgggtcag gctcatcctg ctcgacaccg
     3721 acctgccgga gaacagcgac cacgatcgct tcatcgcgca tcggttgtac ggcggcgatc
     3781 gcggcacgcg catcgagcag gagatcgtgc tcggcatggg cggcgtgcgc gcgctcgcgg
     3841 cgctgaatct tcg
//
LOCUS       DBNHBY010000578        12413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1594924, whole genome shotgun sequence.
ACCESSION   DBNHBY010000578 DBNHBY010000000
VERSION     DBNHBY010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1594924"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="AC16HB_14090"
     CDS             <1..119
                     /locus_tag="AC16HB_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR5607964.1"
                     /translation="RLDSGDLRAIVKEIDGQQLKPGDWVRLLSEGGVTRVTR"
     gene            complement(166..714)
                     /locus_tag="AC16HB_14095"
     CDS             complement(166..714)
                     /locus_tag="AC16HB_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5607965.1"
                     /translation="MLFIEIARMLIETAAALLGVTLLLRAYMNWLGMPARNPLAQFVL
                     ALTDWVVVPLRRIVPAFRRIDSASLVGAYLVAVLQLVLIFLVIGAGVADWPWAKVLLV
                     SLAQLLRWALYLVMWLTIIHVILSWVNPYAPVAPAMSMLVRPFLAPFQRIIPTVGGVD
                     LSPVLLIVVVNVLLLVFANAGM"
     gene            complement(824..1183)
                     /locus_tag="AC16HB_14100"
     CDS             complement(824..1183)
                     /locus_tag="AC16HB_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HIR5607966.1"
                     /translation="MATAKKAAKKTAKKAAKKKTAKKKTAKKKTAKKAAKKKTAKKAA
                     KKAAKKKVAKKKPAKKAAKKKAAKKPAPKKAAAPKAQPAAAPAPAATTPSTAAAPGAK
                     TALNPAAAWPFPTGSRP"
     gene            complement(1756..2889)
                     /locus_tag="AC16HB_14105"
     CDS             complement(1756..2889)
                     /locus_tag="AC16HB_14105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR5607967.1"
                     /translation="MTIRAESDMLTWDDRPGTNLCLQPVEPASTASAKRGPDLRRVNV
                     ADKRIINGATDVNQLVPFKYKWAWEKYLAACANHWMPQEINMSRDIALWKDPNGLTED
                     ERRVVRRNLGFFVTADSLAANNIVLGTYRHITAPECRQFLLRQAFEEAIHTHAYQYIV
                     ESLGLDEGEIFNAYHEVPSIRAKDEFLIPFIDTLTDPDFRTGTIEADQALLKSLIVFA
                     CLMEGLFFYVGFAQILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQIKLENPQ
                     LWTPAFRDEIRALFQTAVDLEYAYAADTMPRGVLGLNAPMFKGYLRFIANRRAQQIGI
                     DALFHAEDNPFPWMSEMIDLKKERNFFETRVIEYQSGGALSWE"
     gene            complement(2886..5774)
                     /locus_tag="AC16HB_14110"
     CDS             complement(2886..5774)
                     /locus_tag="AC16HB_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR5607968.1"
                     /translation="MQYQETSAVLATPPTGLRPAPGIAAGNVMATSFADYKIIRRNGA
                     VVAFEPSKIAVAMTKAFLAVRGGQGAASAAIREQVERLTASVVAALLRRQPAGGTFHI
                     EDVQDQVELSLMRAGEHDVARAYVLYRERRAQERAAKAKEQAPAAPQLSMLENGQRVP
                     LDMPRVRALVESASVGLNDFVSAEAILAETVKNLYDGVPLDEVYKSAILAARALIEKD
                     PAYSYVTARLLLHTIRHEALGEEVTHAQMTLRYAEFFPKFIKYGIEAELLDERLALFD
                     LKQLARALQPERDLQFNYLGLQTLYDRYFLHHEGRRFELPQAFFMRVAMGLALNEVDR
                     EARAIEFYEVLSRFDFMSSTPTLFNSGTRHSQLSSCYLSTVNDDLDGIYETIKENALL
                     AKYAGGLGNDWTPVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCCY
                     LESWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMKRVQEGGDWTLFSPSDCPD
                     LHDKCGRAFDEAYVRYEDKAARGEIKLYKKIPALSLWRKMLSMLFETGHPWITFKDAC
                     NVRSPQQHVGRIHSSNLCTEITLNTSDSEIAVCNLGSVNLPAHMVPGGAGGYVLDHAR
                     LKATIATAMRMLDNVIDINYYAVPKARNSNVRHRPVGLGLMGFQDCLHLMRTPYASPA
                     AVEFADRSMEAVCYYAYWASTELAQERGRYSSFAGSLWDRGILPQDTLELLAEQRGGY
                     VEVDTSATLDWGALRSRIKRYGMRNSNCVAIAPTATISNIIGVDACIEPTFQNLFVKS
                     NLSGEFTVVNEHLVRDLKKLGLWDEVMVADLKYFDGSLAKIDRIPADLRQLYATAFEI
                     DPVWLIECAARRQKWIDQAQSLNIYMAGASGKKLDETYRLAWMRGLKTTYYLRTIGAT
                     HAEKSTTSRAGSLNAVRSDGGLVAAPADGKVCMLKPGDPGFADCEACQ"
     gene            complement(5966..6550)
                     /gene="ampD"
                     /locus_tag="AC16HB_14115"
     CDS             complement(5966..6550)
                     /gene="ampD"
                     /locus_tag="AC16HB_14115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR5607969.1"
                     /translation="MSAAAAPSIDGDGWLAGARRCPSPNCDARPPDTVIDLLVIHNIS
                     LPPGDFGGGAIEALFTNRLDPQAHPFFAAVAGLRLSSHFLIERDGRVTQFVSCLARAW
                     HAGASNFEGRRGCNDYSIGIELEGTDFTWFAPAQYVALAFLVRALRAHFSLRAVRGHQ
                     HIALERKTDPGPFFDWQRLANEAALPAALLPPID"
     gene            complement(6547..8016)
                     /locus_tag="AC16HB_14120"
     CDS             complement(6547..8016)
                     /locus_tag="AC16HB_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5607970.1"
                     /translation="MSDAKSSGEVAPHRMPAVLVVDDEADLRELLGLTLVRLGLDVDA
                     AESLGRARELLATHRYALCLADMRLPDGVGLDLVREIAQGRYGDAVPVAVITAYGSAE
                     NAVAALKAGAFDYLTKPVDLDQLRLLVRSALAGGAAPQEPARVPVEERFATRLLGNAP
                     SMVQLRAMIERLARSMAPVAVYGESGSGKELIARAIHDTSARRAQPFVAVNCGAIPDT
                     LMEAEFFGYRKGAFTGADRDRDGFFQAASGGTLFLDEVAELPLAMQVKLLRAIQERRV
                     RKVGATAEEPVDVRILSATHQDLARLVAEGRFRQDLFYRLNVIELRAPSLRERIEDVP
                     LLADAILKRLAERTSSAPAHLAPPALAALHRYSFPGNVRELENILERAVALAADGELG
                     PEDLMLPAGEGALAAPMEAATEEAGRDDASEVAAVAARGFDVVDGVPNDLQAYVDALE
                     RDAIRAALEKTRFNRTAAAQLLGITFRQLRYRMQRLGVK"
     gene            complement(8013..9662)
                     /locus_tag="AC16HB_14125"
     CDS             complement(8013..9662)
                     /locus_tag="AC16HB_14125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5607971.1"
                     /translation="MTSPDADESAWRTLEYFALTRALIASALVLSVVALGAGPSGWHA
                     TPVAIGSIAYFVLSAVLALSAIYMRKRFFAQVALQIAVDLIVISSLLMLGGGSRTGVT
                     ILYLLPLAGASLLLPTIAVFFVCALAVLAILLDAVLRLLDAEGSSAIVFQAGLYGAAL
                     FAVAALLRFLASRLAVQERLAQSRGRMIESQLEINRLVIAQMEQGAIVVDATTRVRAN
                     NRGARILLGLGPDAQLTGRQLTEFAALAPLSSALQRWIDVISRSGEGRSEETFALPEL
                     KLRARFVRPHATTSDEFAIFLEDMRRVEERAQQLKLAAMGRLTASIAHEIRNPLGAIG
                     HAAQLLCEDVRDPTAQRLAAIVRENTQRLNRLVDDVLRAARREPPLGDDIDLTTFIAS
                     WRTEFMRDRGLDPAIVAVEVAPGLRVRFEESHLRQVLYNLVDNALRFASGAPGSVLLL
                     AERADDERAAALLWVFDDGPGIPADMSVNLFEPFFSTGARGTGLGLYMAREFCVVNRA
                     ELGHTVRRSGPSERAGFVIRFPASTGARLDTAGFLDTIPAP"
     gene            complement(9690..9935)
                     /locus_tag="AC16HB_14130"
     CDS             complement(9690..9935)
                     /locus_tag="AC16HB_14130"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HIR5607972.1"
                     /translation="MGRILFWLLLALAAYIGYRWLRVKQQPREVRGAGGPDRVETMVR
                     CDVCGLNLPQSEAIGAADRWYCSEEHRSRAARGSGEG"
     gene            complement(9935..10774)
                     /locus_tag="AC16HB_14135"
     CDS             complement(9935..10774)
                     /locus_tag="AC16HB_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR5607973.1"
                     /translation="MESGAFYVLGYAVVAVLYLLMAAINWRAVQTGETVMWSRWRGWM
                     IPAIAAGHLVLLAHAVVGGGEFRFGFAHALSATLLLAVLIIWVEGFFVPMRGLDMLVL
                     PAAALAVPLPGLFHGAAISAAADTGALRVHLVMAISAYSLLTIAALHALLMASVDRQL
                     HAALGPPDRTSRWLAQMPPLLAMERMLFRLIAAGFVLLTLTLISGVFFSEALFGRALR
                     FDHKTVFTIASWFVFAGLLAGRFVFGWRGRKALRWTLAGFLMLLLAYVGSRFVLEVVL
                     HRI"
     gene            10820..12211
                     /gene="ffh"
                     /locus_tag="AC16HB_14140"
     CDS             10820..12211
                     /gene="ffh"
                     /locus_tag="AC16HB_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5607974.1"
                     /translation="MLETLTERLSRVVKQMRGQARLTEANTQEMLREVRVALLEADVA
                     LPVVRDFIGRVREKALGEEVVGSLTPGQALVGVVQRELTQLMGGDLPAESRELNLATQ
                     PPAVVLLAGLQGAGKTTTAAKLAKWLRDERKKKVLTVSTDVYRPAAIEQLKTVTAQAG
                     AEFFPSAGEDQPLDIAARALEHARRHFFDILIVDTAGRLAIDEAMMQEVAALHAGLHP
                     IETLFVVDAMLGQDAVSTAKSFSERLPLTGVILTKLDGDARGGAALSVHHVTGKPVKF
                     VGVAEKIGGLERFDPERMAGRILGMGDIVALVEDVRKSIDVKAAEKFAQRMQSGERFD
                     LNDFRDQIGQMRKMGGLSSLLDKLPAQFAQAAGQVNDKDAERQIRRVEGIINSMTPQE
                     RAKPDLIKASRKRRIAAGSGVPVQEVNRMLNQFEQMQAMMKQMKKGGLAKMMRAMGGL
                     KGIGRGMPGAPKR"
     gene            complement(12231..>12413)
                     /locus_tag="AC16HB_14145"
     CDS             complement(12231..>12413)
                     /locus_tag="AC16HB_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607975.1"
                     /translation="FRIGENLLLNLELERVQIQQPEDKTKTNRRIACRVRPDSTLWSA
                     SAGSSVRSTNEPAGGE"
BASE COUNT         2202 a         4096 c         4033 g         2082 t
ORIGIN      
        1 tcaggctcga tagcggtgac ctgcgcgcga tcgtcaagga aatcgacggc cagcagttaa
       61 agccgggcga ctgggtgcgg ctgctcagcg agggcggcgt cacgcgcgtg acgcgttagg
      121 tacttcgcga cgggcaaccg aacaggttgc cagcctctcg ttccgttaca tcccggcgtt
      181 cgcgaacacc agcagcaata cgttgacgac cacgatcagc agcaccggcg aaaggtcgac
      241 gccaccgacg gtcgggatga tgcgctggaa cggcgccagg aacggacgca cgagcatcga
      301 catcgccggc gcgacgggcg cgtaggggtt cacccagctc aggatcacgt ggatgatcgt
      361 gagccacatc acgagataaa gcgcccaacg caacagctgc gccagcgaga ccagcaggac
      421 ctttgcccac ggccaatcgg caactccggc tccgatcacc aggaagatca gcacgagttg
      481 gagcacggca acgaggtatg ccccgaccag cgacgccgag tcgatgcgcc ggaaggccgg
      541 aacgatccga cgcagcggca cgacgaccca gtcggtcagg gcgagtacga actgtgcgag
      601 cggattgcgc gccggcatgc cgagccagtt catgtacgcg cgcagcagca gagtgactcc
      661 caggagcgcg gccgccgtct cgatcagcat ccgcgcgatt tctatgaaga gcattcgtcg
      721 ttccgcggcc acatgaggcg accgatttta gccatccggc tcggaacgcc ggccaagaaa
      781 aagggcgctg agagtctcag cgcccgagtt tgcatgcgaa gcgttatggc cggctgcccg
      841 ttgggaaggg ccacgccgca gcggggttga gcgcggtctt cgcgcccgga gccgctgcgg
      901 tcgacggcgt tgtcgccgcc ggagccgggg ctgctgctgg ttgagccttg ggtgcagcag
      961 ccttctttgg agccggcttt ttggccgcct tcttcttggc ggccttcttc gcgggttttt
     1021 tcttagctac tttttttttt gccgctttct tagcggcctt ttttgcagtc ttctttttcg
     1081 ccgctttctt ggcagtcttc ttcttcgccg tctttttctt ggcggttttt ttcttggcag
     1141 cttttttcgc ggttttcttc gcagctttct tggctgttgc catttgatgc tcctttaggt
     1201 cagtcagagt ggttgaggat gaaacccgcg cgacccggaa gggtcgttcg ggctattcat
     1261 cggcgcacgc agatgctctg tcgccgcgac aaccccagaa gtgcgtttga ggcgcagatc
     1321 cgagaagagc gcggaacggg cagcgcactg accgctgacg ctgatgaatc cggtgacgtc
     1381 cgcttcggcg ccgtggcggc ggagcgggcg aaatacgaag aaaccgccgg gagcggcaaa
     1441 tgaaaacgcc ggcggaaagc cggcgtcgag tgaactgggg cgggaaagct gctgccggaa
     1501 gcctcgggct tttgcagagg gagcgactcc acaagactct tgcgtcctgc tgtcgactcg
     1561 tctgctgtct ctgacgatga accttgaact atgactgtct gtgttcaatt cagcgatcga
     1621 tcgtgtttgt tagaggcact gatcgagcaa ccaaactgag gttgcttttt actctcaatc
     1681 aaattggatc gcaagaaatt tttcgcatga tcacgcgttg tgttgtgtgt tgtgcgcgat
     1741 tcttctccgc gcacatcact cccacgacag cgcgccaccc gactgatact cgatcacgcg
     1801 cgtctcgaag aagttgcgct cctttttcag atcgatcatc tcgctcatcc acggaaacgg
     1861 gttgtcctcc gcgtggaaca gcgcgtcgat gccgatctgc tgcgcgcgcc ggttggcgat
     1921 gaagcgcagg tagcccttga acatcggcgc attgaggccc agcacgccgc gcggcatggt
     1981 gtccgcggcg tacgcgtact ccaggtcgac ggcggtctgg aagagcgcgc ggatctcgtc
     2041 gcggaaggcc ggtgtccaga gctgcgggtt ctccagcttg atctggttga tgaggtcgat
     2101 gccgaagttg cagtgcatcg actcatcccg aaggatgtac tggtactgct cggccgcgcc
     2161 ggtcatcttg ttctgccggc caagcgccag gatctgcgcg aaaccgacgt agaagaacag
     2221 gccctccatc aggcaggcga acacgatcag cgacttcagc agcgcctggt cggcctcgat
     2281 cgtgccggtg cggaagtccg gatccgtcag cgtgtcgatg aatgggatca ggaactcgtc
     2341 cttggcgcgg atcgagggga cctcgtggta tgcgttgaat atctcgccct cgtccaggcc
     2401 gagcgattcg acgatgtact ggtacgcgtg ggtatggatc gcctcctcga acgcctgccg
     2461 cagcaggaac tggcggcact ccggcgccgt gatgtgccga taagtgccca gcacgatgtt
     2521 gttggcggcc agcgagtcgg cggtaacgaa gaagccgagg ttgcgcctaa cgacccggcg
     2581 ctcgtcctcg gtcagaccgt tggggtcctt ccacagcgcg atgtcgcggg acatgttgat
     2641 ctcctgcggc atccagtgat tagcgcaggc agccagatac ttctcccaag cccacttgta
     2701 cttgaacggc accagctggt tgacgtcagt ggcgccgttg atgatccgtt tgtcggcgac
     2761 gttgacccgc cgcagatcgg gtccgcgctt ggcggacgcc gtcgatgcgg gttcgacggg
     2821 ctgcaagcaa aggttggttc cgggccgatc gtcccaggtc agcatgtcgg attccgctcg
     2881 aattgtcatt ggcaggcttc gcagtcggca aatcccggat cgcccggctt cagcatgcag
     2941 actttgccgt cggcaggcgc ggcgacgagc ccgccatcgg accgcacggc attgagcgaa
     3001 ccggcgcgcg acgtggtgga cttctccgcg tgcgtcgcac cgatcgtgcg caggtagtaa
     3061 gtcgtcttca gcccacgcat ccaggccagt ctgtaggtct cgtcgagctt cttgccggag
     3121 gcgcccgcca tatagatatt gagggactgc gcctggtcga tccatttctg ccggcgtgcc
     3181 gcgcattcaa tcagccagac ggggtcgatc tcgaatgcgg tcgcatagag ctgccgcagg
     3241 tcggcgggga tgcggtcgat cttcgccagg ctgccgtcga agtacttcag gtccgcgacc
     3301 atcacctcgt cccacagacc gagcttcttc aaatcccgca ccaggtgctc gttcacgacc
     3361 gtgaattcgc ccgacaggtt cgatttgacg aacaggttct ggaatgtcgg ctcgatgcag
     3421 gcgtcgacgc cgatgatgtt ggagatcgtc gccgtcggcg cgatcgccac gcagttggaa
     3481 ttgcgcatcc catatcgctt gatccgggat cgcagcgccc cccagtccag cgtggccgac
     3541 gtatcgacct ctacataccc gccgcgctgc tcggccagca gctctagcgt gtcctgcggg
     3601 aggatgccgc gatcccacag cgagccggcg aacgacgaat agcggccgcg ctcctgagcc
     3661 agctcggtcg acgcccagta cgcgtagtag cagaccgctt ccatgctgcg gtcggcgaac
     3721 tcgaccgcgg ccggcgatgc gtagggcgtg cgcatcaggt gcaggcagtc ctggaagccc
     3781 atgaggccaa ggccgaccgg acgatggcgg acgttggagt tgcgcgcctt cggcaccgcg
     3841 tagtagttga tgtcgatcac gttgtcgagc atccgcatcg cggtcgcgat cgtggccttt
     3901 agcctcgcgt gatcgaggac gtagccgcct gcgccgcccg ggaccatgtg cgccggcagg
     3961 ttgaccgagc ccaggttgca caccgcgatt tcggagtcgg acgtgttgag cgtgatctcg
     4021 gtgcacaggt tcgagctgtg aatccgacct acgtgctgct gcggcgagcg cacgttgcag
     4081 gcgtccttga acgtgatcca cgggtggccc gtttcgaaca gcatcgacag catcttgcgc
     4141 cacagcgaca gcgccgggat cttcttgtag agcttgatct cgccgcgtgc ggccttgtcc
     4201 tcgtagcgga cgtaagcctc gtcgaacgcg cggccgcact tgtcgtgcag gtcggggcag
     4261 tcgctgggcg agaacagcgt ccagtcgccc ccttcctgca cccgcttcat gaacagatcc
     4321 ggaatccagt tcgcggtgtt catgtcgtgt gtacggcggc ggtcgtcgcc ggtgttcttg
     4381 cgcagttcga ggaactcctc tatgtcgagg tgccaggact cgagatagca gcacaccgca
     4441 cccttgcgct tgccgccctg gttgaccgcg acggcggtgt cgttgaccac cttcaggaac
     4501 ggaacgacac cctggctctt gccgttggtt cccttgatgt gcgaacccag cgcgcgcacg
     4561 ggcgtccagt cgttgccgag gccgccggcg tacttggcga gcagcgcgtt ctccttgatc
     4621 gtctcgtata tgccgtccag atcgtcgttg acggtcgaca gatagcagga cgatagctgc
     4681 gagtgccgcg tgccgctgtt gaacagcgtc ggcgtcgaac tcatgaagtc aaacctcgag
     4741 agcacctcgt agaactcgat cgcgcgcgcc tcacggtcga cctcgttcag cgccagcccc
     4801 atcgccacgc gcatgaagaa ggcctgtggc aattcgaagc ggcgcccctc gtggtgcagg
     4861 aaatagcggt cgtacagcgt ctgcagaccg aggtagttga actgcaggtc gcgttccggc
     4921 tgcagtgccc gcgccagctg cttcaggtcg aacagtgcga gcctttcatc cagcaactcg
     4981 gcctcgatcc cgtacttgat gaacttcgga aagaactctg catagcgcag cgtcatctgc
     5041 gcgtgcgtca cttcttcgcc cagcgcctcg tgtctgatgg tgtgcagcag cagccgcgca
     5101 gtcacgtacg aatacgccgg gtccttctcg atcagcgcgc gcgcggccag gatcgcagac
     5161 ttgtagacct cgtccagcgg tacgccgtca tacaggttct tgaccgtctc ggccaggatc
     5221 gcttcggccg acacgaagtc gttcaggccg acgctggccg actcgaccag cgcacggacg
     5281 cgcggcatgt cgagcggcac gcgctggccg ttctccagca tcgaaagctg cggcgcggcc
     5341 ggcgcctgct ccttcgcctt ggctgctcgc tcctgcgcac gccgctcgcg gtaaagcacg
     5401 tatgcgcgcg cgacgtcgtg ctcgccggca cgcatcaggg acagttcgac ctggtcctgc
     5461 acgtcctcga tgtggaaagt gccgccggcc ggttggcgcc gcagcaaagc cgccaccaca
     5521 ctcgccgtca acctctcgac ctgctcccgg atcgccgccg aggccgcgcc ctgcccgcca
     5581 cgcactgcga ggaacgcctt ggtcatcgcc accgcgatct tggatggctc gaaggcgacg
     5641 actgcgccgt tgcgccggat gatcttgtag tcggcaaacg acgtcgccat cacgttgccg
     5701 gccgcaattc ccggcgccgg gcgcaagccg gtcgggggcg tggcaaggac cgcgctcgtc
     5761 tcctgatact gcatggggcc tccgggtctc cgatcgtggg cgcggccgtg tccgccgcgc
     5821 tgtgccagga accgcaagat ataggggtct ggctgtattg ccgcacaaca aattgtagcg
     5881 ggcgatctta ggcagagttg ccgtggaacg caagccgaaa aaatgtgcgc tgcatgcgcg
     5941 caacgcgccg ggcgccgcct ccgcttcagt cgattggcgg cagcagcgcc gccggcagcg
     6001 cggcttcgtt cgccagacgc tgccagtcga agaagggacc gggatcggtc ttgcgttcga
     6061 gtgcgatgtg ctgatggccg cgcaccgccc gcagcgaaaa gtgcgcccgc agcgcgcgca
     6121 ccagaaacgc gagcgccacg tactgcgccg gcgcgaacca cgtgaagtcc gtgccctcca
     6181 gctcgatgcc gatcgagtaa tcattgcagc cgcgacgacc ctcgaaattg gacgccccgg
     6241 cgtgccatgc acgtgccagg cacgacacga attgcgtgac gcggccgtcg cgctcgatca
     6301 ggaagtggct cgacaggcgc aggccggcga ccgcggcaaa gaacgggtgg gcctgcgggt
     6361 cgagccggtt ggtgaacagt gcctcgatcg cgccgccgcc gaagtcgccc ggcggcaggc
     6421 tgatgttgtg gatcaccagg aggtcgatca ccgtgtccgg cggccgcgca tcgcagttcg
     6481 gcgacgggca gcgacgcgcg cccgccagcc agccatcgcc atcgatcgac ggcgcggcgg
     6541 ccgcgctcat ttgacgccga gccgttgcat ccggtaccgc aactggcgga acgtgatgcc
     6601 gagcagttgc gcggcagcgg tacggttgaa gcgtgtcttc tcgagcgccg cccggatcgc
     6661 gtcgcgttcg agcgcatcga cgtaagcctg caggtcgttt ggcacgccat cgaccacatc
     6721 aaagccgcgc gcagccacgg cggcgacctc cgaagcatcg tcgcgcccgg cttcttcggt
     6781 ggcggcttcc atcggtgccg ccagcgcccc ttctcccgcc ggcagcatca gatcttccgg
     6841 ccccagctca ccatccgccg ccagcgcaac cgcgcgctcg aggatatttt ccaactcgcg
     6901 cacgttgcca gggaacgagt accggtgcag cgccgcgagc gccggcggcg ccagatgcgc
     6961 cggcgccgag ctagtgcgct cggccagccg cttcaggatc gcgtcggcga gcagcggcac
     7021 gtcttcgata cgctcgcgca gactcggtgc acgcagctcg atcacgttca accgataaaa
     7081 gagatcctgg cgaaagcggc cttcggcgac cagccgtgcg agatcctgat gcgtggcgct
     7141 gaggatgcgc acgtcgaccg gctcctccgc cgttgcccca accttgcgca cgcgccgttc
     7201 ctggatcgcg cgcagcagct tgacttgcat cgccagcggc agctcggcga cctcgtcgag
     7261 gaagagcgtg ccgccactcg cggcctggaa gaaaccgtcg cgatcacggt cggcaccggt
     7321 gaatgcaccc ttgcgatagc cgaagaactc ggcctccatc agggtgtccg gtatcgcacc
     7381 gcagttgacg gcaacgaacg gctgcgcgcg ccgcgccgag gtgtcgtgaa tcgcgcgtgc
     7441 tatcagttcc ttcccgctgc ccgactcgcc gtacacggcc accggcgcca tgctgcgcgc
     7501 cagccgttcg atcatcgcgc gcaactgaac catcgacggc gcattgccga gcagtcgcgt
     7561 ggcaaaacgc tcctcgacag gcacccgcgc cggctcctgc ggcgcggcgc caccggcgag
     7621 cgcagagcgc accagcaaac gcaactgatc cagatccacc ggcttggtca gatagtcgaa
     7681 cgcgccggct ttgagcgcag cgactgcgtt ttccgcgctg ccgtaagcgg tgatcaccgc
     7741 gactggaacg gcgtcgccgt accgcccctg ggcaatttcg cgcacgaggt cgagcccgac
     7801 gccgtcgggc aatcgcatgt cggccaggca cagcgcgtag cggtgcgtcg ccagcagttc
     7861 gcgcgcgcgc ccgagggact cggcggcgtc gacgtccaga ccgagccgca ccagcgtcag
     7921 gcccaacagt tcgcgcaggt ccgcttcgtc gtcgaccacc agcaccgccg gcatccggtg
     7981 cggtgcgact tcgcccgaag atttggcgtc gctcatgggg ccgggatcgt atcgaggaaa
     8041 ccggccgtgt cgagtcgcgc gccggtactg gccgggaagc ggatcacgaa gccggcccgc
     8101 tccgatgggc cgctgcgccg caccgtgtga ccgagctcgg cgcggttgac gacgcagaac
     8161 tcgcgcgcca tgtacaaccc cagaccggtg ccacgggcac cggtgctgaa gaacggctcg
     8221 aacaggttca cgctcatgtc ggctggaata ccggggccgt cgtcgaacac ccagagcagc
     8281 gcggcggcgc gttcgtcgtc cgcccgctca gccagcagca gcacgctgcc tggcgcgccg
     8341 gacgcgaaac gcagcgcatt gtcaaccaag ttgtaaagca cctgccgcag atgcgattct
     8401 tcgaaacgca cacgcagccc cggcgcgact tcgacggcga cgatggcggg atcgagtccg
     8461 cggtcgcgca tgaattcggt ccgccagctc gcgatgaagg tcgtaaggtc gatgtcatcg
     8521 ccgagcggcg gttcgcgacg cgccgctcgc agcacgtcgt cgaccagccg gttcagccgc
     8581 tgcgtgtttt cgcgaacgat ggcagccagg cgctgcgcgg tcgggtcgcg tacgtcttcg
     8641 cacagcagtt gcgctgcgtg cccgatcgcg ccgagcggat tgcgaatctc gtgcgcaatc
     8701 gaggccgtca gccgccccat cgcagccaac ttcaactgct gcgcccgttc ctcgacccgg
     8761 cgcatgtcct cgaggaagat cgcgaactcg tccgaggtgg tcgcgtgcgg gcgcacgaaa
     8821 cgcgcgcgca acttcagttc cggcaacgcg aacgtctctt ccgagcgccc ctcgccgctg
     8881 cgcgagatca cgtcgatcca gcgctgaagc gcggaagaca acggtgccaa ggcggcaaat
     8941 tcggtcagtt gccggcccgt cagctgcgca tcgggcccca gcccgagcag gatgcgcgcg
     9001 ccacggttgt tggcgcgcac gcgggtcgtc gcatcgacga cgatcgcgcc ctgctccatc
     9061 tgcgcgatca cgaggcggtt gatttcgagt tggctctcga tcattcgccc gcggctttgc
     9121 gccagccgct cctgcaccgc cagccgcgac gccaggaaac gcagcagcgc ggccactgcg
     9181 aagagcgcgg cgccgtacaa ccccgcctgg aacacgatgg cgctgctgcc ttcggcatcc
     9241 aacagccgca gcacggcgtc gagcagtatc gccagcacgg cgagcgcgca gacgaagaac
     9301 accgcaatgg tcggcaacag cagcgacgcg ccggccagcg gcagcaagta caggatcgtg
     9361 acgccagtgc ggctgccgcc gccaagcatc agcagactcg agataacgat caagtccacg
     9421 gcgatctgca acgcgacctg cgcgaagaag cgcttgcgca tgtagatcgc gctcagcgcg
     9481 agcacggccg acagcacgaa gtacgcaatg ctgccgatcg ccaccggcgt ggcgtgccag
     9541 ccggaggggc cggccccgag agcgacgacc gacagcacca gagcgctggc gatcagcgcc
     9601 cgcgtcaacg cgaagtattc gagcgtgcgc caggccgact cgtcggcatc gggcgatgtc
     9661 atgtgcgccg gttcgtggac cgccgacaat caaccctcgc cgctgccgcg cgccgcccga
     9721 ctgcgatgct cctcgctgca gtaccagcgg tcggccgcgc cgatcgcttc cgactgcggc
     9781 aggttgagac cgcacacgtc gcagcgaacc atggtctcga ccctgtccgg tccgccggcg
     9841 ccgcgcacct cgcgcggctg ctgcttgacc cgcaaccagc gatagccgat gtacgcggcc
     9901 agcgcgagca gcagccagaa cagaatgcgt cccatcagat tcggtgcagc acgacctcga
     9961 gcacgaatcg gcttcccacg taggcgagca gcaacatcag gaagccggcc agggtccagc
    10021 gcagcgcctt gcgcccgcgc cagccgaata cgaagcggcc cgccagcaag ccggcgaaca
    10081 caaaccagga cgcgatcgtg aacaccgtct tgtgatcgaa gcgcagcgcg cggccgaaca
    10141 gcgcctcgga gaagaacacg cccgagatca gcgtcagtgt cagcagcacg aagccggcgg
    10201 cgatcaggcg gaacagcatc cgttccatcg ccagtagcgg cggcatttgc gccagccagc
    10261 gagacgtgcg gtcaggcgga ccgagcgccg cgtgcagttg acggtcgacc gaagccatca
    10321 gcagcgcgtg cagcgcggcg atcgtcagca ggctgtaggc cgagatcgcc atgaccagat
    10381 gcacgcgcag tgcgccggtg tcagccgcgg cggaaatcgc cgcgccatga aacagccccg
    10441 gcagcggcac cgccagtgcc gccgccggca acaccagcat gtcgagtcca cgcatcggca
    10501 cgaagaaacc ctcgacccag atgatcagca ccgccagcag cagcgttgcc gacaacgcgt
    10561 gcgcgaagcc gaagcggaat tcgccgccgc cgaccactgc gtgcgccagc aggaccagat
    10621 ggccggcggc gatcgccggg atcatccaac cgcgccagcg cgaccacatg acggtttcgc
    10681 cggtctgcac cgcgcgccag ttgattgccg ccatcagcag gtaaagcacc gccacgaccg
    10741 cgtaacccag cacgtagaat gcccctgatt ccatgcttct tatatagcac gcggacagcg
    10801 cgagccgcgg ctcccctgaa tgctcgaaac tctgactgaa aggctctctc gcgtcgtcaa
    10861 acagatgcgc gggcaggcgc gcctgaccga ggccaacacc caggagatgc tgcgcgaggt
    10921 ccgcgtggcc ttgctcgagg cagacgtcgc cttgccggtg gtgcgcgatt ttatcgggcg
    10981 cgtcagggaa aaggcgctcg gcgaagaggt ggtcggatca ctcacgccgg ggcaggcgct
    11041 ggtcggtgtc gtgcagcgtg aactgacgca gttgatgggc ggcgacctgc cggccgagtc
    11101 gcgcgagttg aatctcgcca cgcagccgcc tgccgtcgtc ctgctggccg ggctgcaggg
    11161 cgccggcaag accaccaccg cggccaagct cgcgaaatgg ctgcgcgacg agcgcaagaa
    11221 gaaggtgctg acggtctcga ccgacgtgta ccgtccggcc gcgatcgagc agttgaaaac
    11281 ggtgacggcg caggcgggcg cggaattctt tcccagcgcc ggcgaggatc agccgctcga
    11341 catcgccgcg cgcgcgctcg agcacgcgcg ccggcacttc ttcgacattc tgatcgttga
    11401 taccgccgga cggctggcaa tcgacgaggc aatgatgcag gaggtcgccg cgctgcacgc
    11461 ggggctgcat ccgatcgaga ctctgttcgt ggtcgacgcg atgctgggcc aggacgcggt
    11521 cagcaccgcc aagtcgttct cggagcgcct gccgctcacc ggcgtgatcc tgaccaagct
    11581 cgacggcgat gcgcgcggcg gcgcggcgct gtctgtgcac catgtgaccg gcaagccggt
    11641 caagttcgtc ggcgtggcgg agaagatcgg cgggctggaa cggttcgacc cggagcgcat
    11701 ggccggccgc atcctcggta tgggcgacat cgtcgccttg gtcgaggacg tgcgcaaatc
    11761 gatcgacgtg aaggcggccg agaagttcgc gcagcgcatg cagtcgggcg agcgcttcga
    11821 cctgaacgac ttccgcgatc agatcggcca gatgcgcaag atgggcggac tgtcgagcct
    11881 gctcgacaag ctgccggcac agttcgcgca ggcggctggc caggtcaacg acaaggatgc
    11941 cgagcgccag atccgccgcg tcgaaggcat catcaactcg atgacaccgc aggaacgcgc
    12001 caagcccgac ctgatcaagg cctcgcgcaa gcgccgcatc gccgccggtt ccggtgtacc
    12061 ggtgcaggaa gtcaaccgca tgctcaatca gttcgagcag atgcaagcga tgatgaagca
    12121 gatgaagaag ggcggcctgg cgaaaatgat gcgcgcgatg ggcggtctga aaggcatcgg
    12181 acgcggcatg cctggcgcgc cgaagcgctg acccacgaca cgaggcaagg tcattcaccg
    12241 cccgccggct cattcgtcga tctgacgctg ctgcctgcgc tggccgacca cagtgtcgaa
    12301 tcgggcctca ctcggcacgc gatccttcgg ttggtcttgg tcttgtcctc tggctgctgg
    12361 atctgcaccc gctcgagctc gagattcaag agcaggttct cgccgatgcg aaa
//
LOCUS       DBNHBY010000579         1792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1258152, whole genome shotgun sequence.
ACCESSION   DBNHBY010000579 DBNHBY010000000
VERSION     DBNHBY010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1258152"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1351
                     /gene="glpK"
                     /locus_tag="AC16HB_14150"
                     /pseudo
     CDS             1..1351
                     /gene="glpK"
                     /locus_tag="AC16HB_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247548.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
     gene            1348..>1792
                     /locus_tag="AC16HB_14155"
     CDS             1348..>1792
                     /locus_tag="AC16HB_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5607976.1"
                     /translation="MNAALAPLPIERGDLLARLREPTTWDAVVIGGGATGLGIALDAA
                     TRGLRVALIDAQDFAAGTSSRSTKLVHGGVRYLAQGNLKLVREALEERAILLAIAPHI
                     VHPLEFIVPCYRPFEREYMRIGLGMYDLAAGRFSIGPTRALSAAET"
BASE COUNT          247 a          616 c          666 g          263 t
ORIGIN      
        1 ccaatcgggc gtgatgcacg aggcgctggc caaggcgaac ctcggggcgc gcgacctcgc
       61 ggcgatcggc atcaccaacc agcgcgaaac gaccgtggta tgggagcgcg cgaccggccg
      121 cccgatcgcc aacgcgatcg tctggcagga tcggcgcacg gcggatgcct gtgccgcgct
      181 ccgcgcccgc gggctcgagg acatgtttgc gcgcaggacc gggctgctgc tcgacccgta
      241 cttctccggc accaagatcg gctggctgct cgatcacgtg ccaagcgccc gcgtgcgcgc
      301 cgagcgcggc gaactcgcat tcggcacgat cgacagctgg ctcgtatgga acctcagcgg
      361 cggccgcgcg catgtcaccg acgtgagcaa cgcctcgcgc acgctgctgt tcgatctgca
      421 gacgctcgac tgggacgatg agctgctggc gcttctcgcc gtgccgcgcg cgttgctgcc
      481 gcgcgtcgtc gcgtcgagtg cgcgagtcgg cgtcaccgaa cccgcgctct tcggacagga
      541 gatcgtgatc gccggaattg cgggggatca gcaggccgcg accttcgggc aggcatgctt
      601 tgcaccgggc atggcgaaga acacctacgg caccggctgt ttcatgacga tgaatctggg
      661 cgacgcgccg cagccctccg gcgcgcgcct gctcaccacg ctgggctggc atctgggcgg
      721 cgacgccgtc ggcgctgcca cgaccgcgac gttcctgcgc gaaggcagcg tattcgtcgg
      781 cggggcggtg gtgcagtggt tgcgcgacgg gctcgggctg atcgagacgt cggacgagat
      841 cgaggcgctg gcgcgccagg tgcccgacag cggcggcgtg atcctggtgc cggccttcac
      901 cgggctgggc agcccgcact gggacccgca cgcgcgcggc tcgatgatcg gcatcacgcg
      961 cggcaccacg cgcgcgcacg tggcgcgggc ggcgttggag tcgatcgcgt tccagtcggc
     1021 cgagctgctg catgcgatgc agggcgaagc cgcgtgcccg ctgaccgagc tgcgggtcga
     1081 cggcggcgcg gcgcgcaacg atctgctgct gcagttccag gccgacctga tcggcgtgcc
     1141 ggtgctgcgg ccggtcgtga ccgaaaccac cgcgctcggc gccgcctatc tcgcggggct
     1201 cgcggtcggg ttctggtcgg ggctccccga gatcgccgcg cagtggcgga tggagcgcta
     1261 cttctagccg cgcatggcat tggcgacacg cgacgcgttg ctgggcgact ggctgcgcgc
     1321 agtcgaacgg tcgcgcgggt gggtgcaatg aacgctgcgc tggcaccgtt gccgatcgaa
     1381 cgcggagatc tgctggcgcg gctgcgcgag ccgacgacat gggacgccgt cgtgatcggc
     1441 ggcggcgcga cggggctcgg catcgcgctc gacgcggcga cgcgcggcct gcgcgtggca
     1501 ctgatcgacg cgcaggactt cgccgccggc acgtcgagcc gcagcaccaa gctcgtccac
     1561 ggcggcgtgc gctatctggc gcaggggaac ctgaagctcg tgcgcgaggc gctcgaggag
     1621 cgcgcgatcc tgctcgcgat cgcgccgcac atcgtgcatc cgctcgagtt catcgtgccg
     1681 tgctaccggc cgttcgagcg cgagtacatg cgcatcgggc tcggcatgta cgacctcgcg
     1741 gcaggtcgct tcagcatcgg ccccacgcgc gcgctgtcgg cggcggaaac gc
//
LOCUS       DBNHBY010000580         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1123384, whole genome shotgun sequence.
ACCESSION   DBNHBY010000580 DBNHBY010000000
VERSION     DBNHBY010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1123384"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /gene="aceK"
                     /locus_tag="AC16HB_14160"
     CDS             complement(<1..1436)
                     /gene="aceK"
                     /locus_tag="AC16HB_14160"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HIR5607977.1"
                     /translation="MNDLLAATFGARFDHSMPQPPAIARAILEGFDRHYRLFRYVAQQ
                     AKQRFENGDWIGMRESARERIAFYDQRVIEASARVERNFDLATLSDEERDILWQAVKQ
                     HYIALLAEHRQPECAETFFNSVCTKLLHRDYFRNAFIFVRPGVATDYIDSDPPSYRSY
                     YPARHGLKHTIRQILIDFGLACPWVDVERDIRLAIRAVCAAHDRGEAPNALTRPFHVE
                     PDCQIQVLSSLFFRNKGAYVVGRIVNGTRITPLAVPILRDARGQLYIDAVLLSGQHLA
                     TLFSFTRAYFLVDMEAPSAYVDFIRSILPHKPASELYTMVGLQKQGKTLFYRDFLHHL
                     RHSSDQFVIAPGIKGLVMTVFTLPSYPYVFKMIKDVIPFPKETDREQIKGKYQLVKMH
                     DRVGRMADTWEYSQVSLPRARLSPELIQELLAYSASQVELTSETVLIKHVYIERRMTP
                     LNIYLMHASDAEVDHAIREYGQAIKDLAA"
     gene            complement(1433..2083)
                     /locus_tag="AC16HB_14165"
     CDS             complement(1433..2083)
                     /locus_tag="AC16HB_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5607978.1"
                     /translation="MTVAGLSLEKLAVFALAVLLLALTPGPVWIYLLSRTLAQGRRAG
                     YFSLFGVAAGVVMHLLLAAFGLSALLLAIPAAFDAVKLAGAAYLLWLAWSTVRGNGIS
                     FETQPLPPVPDRVLFRQAMVAAAVNPKVAVFYLSLFPQFVDPAAGSVLAQSLVLGVVH
                     ITVGTLVDGALVTLAGVLAAWFATRPLWLRTQRWFLGTAFGALAVWLALTPRHSPQ"
     gene            complement(2265..2771)
                     /locus_tag="AC16HB_14170"
     CDS             complement(2265..2771)
                     /locus_tag="AC16HB_14170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5607979.1"
                     /translation="MHPWLTEMERLRARTAQPGLLDPKLAAGLSGLDFLLRIVRGEIP
                     DPPITRTLDFYLLEVEPGRAVFQGMPAFAHYNPIATVHGGWHGTLLDSAMACAVQTLC
                     EAGRAYTTLEYKVHCVRPLTDRTGPVRAEGKIVASGRRMATAEGRLVDADSKLYSHGT
                     TTCMLFDL"
     gene            complement(2790..>3020)
                     /locus_tag="AC16HB_14175"
     CDS             complement(2790..>3020)
                     /locus_tag="AC16HB_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase"
                     /protein_id="HIR5607980.1"
                     /translation="EIVSLTVTDAQAVAACLIFADAFRVLVEPACGATLAALDAHPER
                     FARFGRVLVEICGGIGVSLGKLAEWRAQFELK"
BASE COUNT          524 a          997 c         1040 g          459 t
ORIGIN      
        1 gccgccaggt ccttgatcgc ctgaccgtac tcgcggatcg cgtggtcgac ctcggcgtcg
       61 gatgcgtgca tcaggtagat gttcagcggc gtcatccggc gctcgatgta cacgtgcttg
      121 atcagcaccg tttccgacgt cagttcgacc tggctcgcgg agtaggcgag cagttcctgg
      181 atcagctccg gcgaaagccg cgcccgcggc agcgacacct gcgagtactc ccacgtgtcg
      241 gccatgcgcc cgacgcggtc gtgcatcttc acgagctgat acttgccctt gatctgctcg
      301 cgatcggtct ccttcggaaa cgggatcacg tccttgatca tcttgaatac atacgggtaa
      361 gacggcagcg tgaacaccgt catcaccagt cccttgatgc cgggcgcgat cacgaactga
      421 tccgacgaat gccgcaggtg gtgcaggaag tcgcggtaaa agagcgtctt gccctgcttc
      481 tgcagtccga ccatcgtgta cagctcgctc gccggcttgt gcgggaggat cgagcggatg
      541 aagtcgacat acgcgctcgg cgcctccatg tcgaccagga agtaggcgcg cgtgaacgag
      601 aacagcgtcg ccaggtgctg gccgctgagc aggaccgcat cgatgtagag ctgcccgcgc
      661 gcatcgcgca ggatcggcac cgccagcggc gtgatgcgcg tgccgttgac aatgcggccg
      721 acgacgtacg cgcccttgtt gcggaagaac aggctcgaca gcacctggat ctggcagtcg
      781 ggctcgacgt gaaacggccg cgtgagcgcg ttgggcgcct cgccgcggtc gtgcgccgcg
      841 cacaccgcgc ggatcgcgag ccggatatcg cgctcgacat cgacccaggg gcaggccagc
      901 ccgaagtcga tcaggatctg ccggatcgtg tgcttcaggc cgtgtctggc ggggtagtag
      961 ctgcggtacg acggcgggtc cgagtcgatg tagtcggtcg ccacgcccgg ccgcacgaag
     1021 atgaatgcgt tgcggaagta gtcacgatgc agcagcttgg tgcacaccga attgaagaag
     1081 gtctccgcgc actcgggctg gcggtgctcg gccagcagcg cgatgtagtg ctgcttgacc
     1141 gcctgccaca gtatgtcgcg ctcctcgtcc gaaagcgtcg cgaggtcgaa gttgcgctcg
     1201 acgcgcgccg acgcctcgat cacgcgctga tcgtagaacg cgatgcgctc gcgcgccgac
     1261 tcgcgcatgc cgatccagtc gccgttctcg aaacgctgct tggcctgctg cgcgacgtag
     1321 cggaacagcc ggtaatggcg gtcgaagccc tcgaggatcg cgcgcgcgat cgccggcggc
     1381 tgcggcatcg aatggtcgaa gcgcgcgccg aaggtcgcgg cgaggaggtc gttcattgcg
     1441 gactgtgccg aggtgtcaat gccagccaca cggcgagagc gccgaatgcg gtgccgagga
     1501 accagcgctg cgtgcgcagc cacagcggcc gcgtcgcgaa ccacgccgcc agaacaccgg
     1561 ccagcgtgac gagggcgccg tcgaccagcg tcccgacggt gatgtgcacc acgccgagca
     1621 cgaggctctg cgccagcacc gagccggcgg cgggatcgac gaactgcggg aacagcgaca
     1681 ggtagaagac cgccaccttc ggattgaccg ctgccgcgac cattgcctgc cggaacagca
     1741 cgcgatcggg caccggcggc agcggctgcg tctcgaacga gatgccgttg ccgcgcaccg
     1801 tcgaccacgc cagccacagc aggtatgccg cgccggcgag tttgactgca tcgaaggcgg
     1861 ccgggatcgc cagcagcagc gccgacagcc cgaacgcggc caggagaagg tgcatgacca
     1921 cgccggccgc cacgccgaac agcgaaaagt agccggcgcg ccgtccctgt gccagcgtgc
     1981 gcgacagcag gtagatccac accgggcccg gcgtcagcgc cagcagcagc accgccagcg
     2041 cgaacacggc cagcttctcg agactcagtc cggcgacggt cataaggaga ccaagtgcag
     2101 ggttgcgccg cgtcgcggca ccaccagggc atctgcggtg agcgcggccg gctcaattct
     2161 cgcgtgaaat gcggccggtg tcccgccgag gcgtgccggc gcagtccttc cggaaaatcg
     2221 cgccggccgc gcgtgcagcc gggccgtgct gcgtcaggcc gtcgttacag gtcgaacagc
     2281 atgcaggtcg tcgtgccgtg cgaatacagc ttgctgtccg catcgaccag ccggccctcg
     2341 gcggtggcca tgcgccggcc gctggcgact atcttgccct cggcgcgcac cggtccggtc
     2401 cggtcggtca gggggcgtac gcagtgcacc ttgtattcga gcgtcgtata ggcacgaccg
     2461 gcttcgcaca gcgtctgcac tgcgcacgcc atggctgaat cgagcagcgt gccgtgccag
     2521 ccgccgtgca cggtggcaat cgggttgtag tgcgcgaacg cgggcatgcc ctggaacacg
     2581 gcgcggccag gctcgacctc gagcaggtag aagtcgagcg tgcgcgtgat cggcggatcg
     2641 ggaatctcgc cgcgcacgat tcgcagcagg aaatcgaggc cgctcaggcc ggcggcgagc
     2701 ttcggatcga ggagcccggg ctgcgcagtg cgggcacgca gccgttccat ttcggtcagc
     2761 cacgggtgca tcgcggctcc tttcgctggt tatttcagtt cgaactgcgc ccgccattct
     2821 gcgagcttgc cgagcgacac gccgatgccg ccgcagatct cgaccagaac gcggccgaac
     2881 cgagcgaagc gctccgggtg cgcatcgagc gcggcgagcg tggcgccgca cgccggctcc
     2941 accagcacgc ggaaggcgtc ggcgaagatc aggcacgcgg cgactgcctg cgcgtcggtc
     3001 acggtgagtg aaacgatttc
//
LOCUS       DBNHBY010000581         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_986953, whole genome shotgun sequence.
ACCESSION   DBNHBY010000581 DBNHBY010000000
VERSION     DBNHBY010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_986953"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="AC16HB_14180"
     CDS             complement(<1..490)
                     /locus_tag="AC16HB_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607981.1"
                     /translation="MSQGSMLPLATVDEAHASLQVISREVTSELNEARAALEAFADKP
                     DDRGALHRFAAHIHLARGALRLAEVYGGALLAEEMEHVARYVDAHSGEGRADSDGLDA
                     LMRAMEQLPAYVERVATGARDLPLGLLPLLNDLRAVRGSALLSEGALLLLNLRSDEPA
                     RPS"
     gene            complement(505..>1614)
                     /locus_tag="AC16HB_14185"
     CDS             complement(505..>1614)
                     /locus_tag="AC16HB_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5607982.1"
                     /translation="NQQAILRLLDELSSLADGDLTVQATVTEDITGAIADSINYAIEA
                     LRELVVTINDSAVKLDAATRQTQALSTHLAKASSAQSKQIGAASESIAAMAASTEEVS
                     GNAERSADVARHSVDIAHKGGDAVRRTIDGMNAIRENIQETSKRIKRLGESSQEIGNI
                     VELINDIAEQTNILALNASIQASMAGEAGRGFAVVADEVQRLAERAANATKQIEVLVR
                     TIQADTNEAVVSMERTTTDVVGGALLAENAGAALEEIEQVSNQIASLVQNISASSRQQ
                     AGVSQSISRNMQVLREISSQTAESTTATSTSIGKLAELAAQLRKSVTGFRLPTSMARS
                     MTGEYPVLRAEEAPPPTEADSSADSRVRRLSSLAG"
BASE COUNT          214 a          539 c          577 g          284 t
ORIGIN      
        1 atgacggacg cgccggctcg tcggagcgca ggttcagcag cagcagtgcg ccctcggaca
       61 gcagggcgct gccgcggacg gcccgcaggt cgttgagcag cgggagcagg ccgagcggca
      121 ggtcgcgcgc gccagtcgcg acgcgctcga cgtaggccgg cagctgttcc atcgcgcgca
      181 tcagcgcgtc gaggccgtcg gaatccgcgc ggccttcacc agaatgcgcg tcgacgtagc
      241 gcgcgacgtg ctccatctcc tcggcgagca gcgcgccgcc gtacacctcg gcgaggcgca
      301 gcgcgccgcg tgccaggtgg atgtgcgcgg caaagcggtg cagcgcgccg cggtcgtccg
      361 gcttgtcggc aaaggcttcg agcgctgcgc gcgcctcgtt gagctccgac gtcacctcgc
      421 gcgaaatgac ctgcagcgag gcatgggcct cgtcgaccgt ggcgagcggc agcatggaac
      481 cctggctcac gctggctgcc ggcgtcagcc ggccaggctg ctgagtcgac gcacgcgcga
      541 atccgcgctg ctgtcggctt cagtgggcgg tggcgcttct tcggcgcgca gcaccgggta
      601 ctcgccggtc atggaccgcg ccatggacgt gggcagccgg aagccggtga ccgacttgcg
      661 caactgcgcc gcgagctcgg ccagcttgcc gatcgacgtc gacgtcgctg tggtgctctc
      721 ggccgtctgc gaactgatct cgcgcagcac ctgcatgttg cgcgagatgc tctgcgacac
      781 gccggcctgc tggcgcgagc tggccgagat gttctgcacc aggctcgcga tctggttgga
      841 gacctgctcg atctcctcga gcgcggcgcc cgcgttctcg gcgagcagtg cgccgccgac
      901 cacgtcggtc gtggtgcgtt ccatggacac gacggcctcg ttggtgtcgg cctggatggt
      961 ccgcaccagc acttcgatct gcttggtcgc gttggcggcc cgctccgcga gccgctgcac
     1021 ttcgtcggcg acgaccgcga agccacgacc ggcttcgccg gccatcgagg cctggatcga
     1081 ggcgttcagc gcaaggatgt tggtctgctc ggcgatgtcg ttgatcagct cgacgatgtt
     1141 gccgatctcc tgcgagcttt cgcccagccg cttgatgcgc ttggacgtct cctggatgtt
     1201 ctcgcgaatg gcgttcatgc cgtcgatggt acggcgcacc gcgtcgccgc ccttgtgcgc
     1261 gatgtccacg ctgtggcgtg cgacgtccgc ggagcgctcg gcattgcccg acacttcttc
     1321 ggtcgaggcg gccatggccg cgatcgattc ggaggcggcg ccgatctgct tggactgggc
     1381 cgacgacgct ttcgcgaggt gagtggacag cgcttgcgtc tggcgtgtcg cggcgtcgag
     1441 cttcacggcg gagtcgttga tggtcacgac aagctcacgc agcgcctcga tcgcgtaatt
     1501 gatcgaatcg gcgatcgcac cggtgatgtc ctcggtgacc gtcgcctgca cggtgaggtc
     1561 gccgtcggcg aggctcgaca gctcgtcgag caggcgcagg atcgcctgct ggtt
//
LOCUS       DBNHBY010000582         2058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_243111, whole genome shotgun sequence.
ACCESSION   DBNHBY010000582 DBNHBY010000000
VERSION     DBNHBY010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_243111"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /gene="gspE"
                     /locus_tag="AC16HB_14190"
     CDS             <1..1693
                     /gene="gspE"
                     /locus_tag="AC16HB_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR5607983.1"
                     /translation="DAPQAVRLGQALVSEGKLSPRDLEHALAAQRETGGLFGRVLIRL
                     GLVSELDVARALSSQLNVPLLLAEAFPPVPPLVDRVAPEFFRSHRILPLDLRDDGLHV
                     VMATPQDEFALRALRLAAGLQIVRYIGLESEIDAALAKTPDTVDAEEAPLEGDIDSAG
                     ASDFVEHLRDLASEAPVIRLVNVLISRVTDLRASDIHLEPFEDGLHVRYRVDGVIQTA
                     EVIEPRLTPAVVSRLKLLAQLNIAERRLPQDGRIKARVKGRELDLRVSCVPTVHGESV
                     VLRVLDRASVRFSLRDLGFSADTLARFETLLTRPHGILLVTGPTGSGKTTTLYGALSK
                     LDAASQKILTVEDPVEYQLDGINQIHVHPQIGLTFANALRSILRQDPDIIMIGEMRDG
                     ETAQIAVQSSLTGHLVLSTLHTNTAASAVIRMQDMGVERYLITSTLNGVLSQRLVRRL
                     CEQCRHPVALPAKVIADTGLGRFLTPGQSVYAAPGCAHCRGTGYLGRTAIHELLIVDD
                     AVRQAIMRGTDAAELQSIARGGGLVSLYEDGLRKVAGGVTSLEEVLRVTQDQSDA"
     gene            1686..>2058
                     /locus_tag="AC16HB_14195"
     CDS             1686..>2058
                     /locus_tag="AC16HB_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5607984.1"
                     /translation="MPDFAYKAADGGGRIIEGQIDAKSRDEALRRLVDQGLTPVGIVD
                     RVVGAASAATAVPAGRAARFRVRPDRVRRADVQVFTSELAVMLRAGLPLDNALRILLN
                     MDQRPPVAALLSEILESVKSGV"
BASE COUNT          317 a          665 c          714 g          362 t
ORIGIN      
        1 ggatgcgccg caggcggttc gactgggtca ggcgctcgtg tccgagggca agctctcgcc
       61 gcgtgacctg gagcacgcgc tggctgcgca gcgcgaaacc ggcgggctgt tcgggcgggt
      121 gctgatccgg cttgggctgg tatccgaact ggatgtggca cgggcgctgt cgagccagct
      181 gaatgtgccg cttttgctcg cggaggcgtt tccacctgtg ccgccgctgg ttgatcgggt
      241 ggcgccggag ttcttccgct cgcatcggat tctgcctctc gatctccgtg acgatgggct
      301 gcacgtcgtg atggcgacgc cgcaggatga gtttgcgcta cgcgcgctgc gtctggcggc
      361 cggcctgcag atcgtccgat atatcgggct cgaatctgaa atcgacgccg cccttgcaaa
      421 gacgcccgat acggtcgatg cggaggaggc gccgctcgag ggcgacatcg acagcgcggg
      481 cgcaagcgac ttcgtcgagc atctacgcga tctcgccagc gaggcgccgg tcattcgtct
      541 ggtcaacgtc ctgatcagcc gcgttacgga tctgcgcgcg tcggacatcc acctcgagcc
      601 cttcgaggac gggctgcacg tacgctaccg cgtcgatggc gtgattcaaa cggccgaggt
      661 catcgagccg cggctgacgc cggcggttgt gtcgcgcctg aagctgcttg cgcagttgaa
      721 catcgcggag cggcgcttgc cgcaggacgg acgcatcaag gctcgcgtca agggacggga
      781 actcgatttg cgtgtttcct gcgtgccgac cgtgcatggc gagagcgtcg tgttgcgggt
      841 gctcgaccgc gcgagcgtcc gcttcagtct gcgcgacctc gggttcagtg ccgatacgct
      901 ggcgcgcttc gagaccttgc tgacgcgccc gcacggcatt ctgctcgtca ccggtccgac
      961 tggatcgggc aagacgacga cgctgtacgg cgcgctgtcc aagctcgacg ccgcctcgca
     1021 gaagatcctg accgtggagg atccggtcga gtatcagctc gatggaatca accagattca
     1081 tgtccatccg cagatcggcc tgacgttcgc gaacgcgctg cgttcgatcc tgcgccagga
     1141 cccggacatc atcatgatcg gcgagatgcg cgacggcgag acggcgcaga tcgcggtgca
     1201 gtcgtccctg accggccatc ttgtgctgtc cacgctgcac acgaacaccg ccgcaagtgc
     1261 ggtgatccgc atgcaggaca tgggcgtcga gcgctacctg atcacctcga ccctcaacgg
     1321 cgtgctctcg caacggctgg tgcgtcgtct gtgcgaacag tgccgtcacc cggtcgccct
     1381 gcccgcgaaa gtcatcgccg acaccgggct cgggcggttt ctcactccgg gccagtcggt
     1441 ctacgcggcg ccgggctgcg cccattgccg gggaacgggg tacctcggac gcacggcgat
     1501 tcacgaactg ctgatcgtcg atgacgccgt gcgtcaggcg atcatgcgcg gcaccgacgc
     1561 ggccgaactg caaagcatcg cgcgcggcgg cgggttggtg tccttgtacg aggacggcct
     1621 gcgcaaggtg gccggcggcg tcaccagcct cgaagaagtc ctgcgggtga cgcaagacca
     1681 gagcgatgcc tgattttgcc tacaaagcgg ccgacggcgg cgggcgcatc atcgaagggc
     1741 agatcgatgc aaagtcccgc gacgaggccc tgcggcgcct ggtcgatcag gggctgacgc
     1801 cagtcggcat cgtcgaccgg gtggtcggcg ccgcatcggc cgcgacggcg gtgccggcgg
     1861 gtcgggcagc gcgattccgt gtgcgtcccg atcgcgtgcg gcgcgccgat gtacaggtgt
     1921 tcacctccga gctcgcggtc atgctgcgcg ccggcctgcc gctcgacaac gcgctgcgca
     1981 tcctgctcaa tatggatcag cggccgccgg tggctgcact gctctccgag atcctggagt
     2041 cggtcaagag cggagtct
//
LOCUS       DBNHBY010000583         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1258721, whole genome shotgun sequence.
ACCESSION   DBNHBY010000583 DBNHBY010000000
VERSION     DBNHBY010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1258721"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="AC16HB_14200"
     CDS             complement(<1..1411)
                     /locus_tag="AC16HB_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5607985.1"
                     /translation="MTRAGSTRTEGETHATPSAAGLRLQLLNTPCLLLATGRIHVLER
                     KDAALLALLAIERKVPRHKAAALLWPDTDDDRAHNNLRQRLFRLRRLAACDVVRSGPV
                     LALAETVAHDLDALRSRVAADSDAAAGQLLGELDYSDCPELLDWLNGARERWRQARCD
                     AFAEVAAQLEAEGQIASALQYAQRLANEDPALEHAHRRVMRLHYLRGDRAAALAAYER
                     CREALRQHVDAGPGPETIALARLIEHSGALVSSVPAPAARPVAVLRPPRMVGREPEWA
                     RLESAWDARRIVIVSGEPGIGKTRLLTDFAFARSGMVVVSARPGDARVPYALLARWLR
                     ACAAQFALPADAWAIGELAHVAPELGAVTDSKADPLRLRQAMLGALATWSANGLAAVT
                     IDDLQFADEATLEMLPALAGETRAALCWLFAVRGNEVPPTVSSWLDACDAEALERLEL
                     RPLTLAATEALLLSLALPQL"
BASE COUNT          244 a          584 c          559 g          237 t
ORIGIN      
        1 cgagctgcgg caatgccagc gagagcagta gcgcttccgt tgccgcgagc gtcaaggggc
       61 gcagttcgag ccgctccaac gcttcggcgt cgcaggcgtc gagccagctg ctcacggtcg
      121 gcggcacttc gttgccgcgc acggcaaaca gccagcacag tgctgcgcgc gtttcaccgg
      181 caagcgccgg cagcatttcg agcgtggcct cgtccgcgaa ttgcagatcg tcgatcgtca
      241 ccgcggccag gccgttcgcc gaccaggtcg ccagcgcgcc cagcatggcc tggcgcaatc
      301 gcagcggatc ggccttgctg tcggtgaccg caccgagttc cggtgcaacg tgcgcgagtt
      361 ctccgatcgc ccacgcatcg gcaggcagcg cgaactgcgc ggcacaggcg cgcagccaac
      421 gggcgagcag tgcgtaaggc acccgcgcgt cgcctggacg cgcactgacg acgaccattc
      481 cgcttcgcgc gaacgcgaag tcggtcagca gtcgcgtctt gccgatgccg ggttcgccgc
      541 tgacgatcac gatgcgacgc gcgtcccagg cactttcgag gcgcgcccac tcgggttccc
      601 ggccgaccat gcgcggcggc cgcagcacgg cgacgggcct ggccgccggc gcgggcacgc
      661 tcgataccag cgcgccgctg tgctcgatca gccgcgccag tgcaatcgtt tccgggcccg
      721 gcccggcgtc gacatgctgg cgcagcgcct cacgacagcg ctcgtacgcg gcgagcgcgg
      781 cggcacgatc gccacgcagg tagtgcagcc gcatcacgcg ccggtgcgcg tgttcgagcg
      841 cgggatcttc attggccagg cgctgcgcgt actgcagcgc tgacgcgatc tgtccttccg
      901 cctccaactg ggccgcgacc tcggcgaacg catcgcagcg cgcctgccgc caccgctcgc
      961 gcgccccgtt gagccagtcg agcagttccg ggcagtcgct gtagtcgagt tcgccgagga
     1021 gttggccggc ggccgcatcg gagtcggcgg ccacccggga tcgcagcgcg tcgaggtcgt
     1081 gcgcaaccgt ctcggccagc gcgagcaccg gcccggagcg caccacgtcg caagcggcca
     1141 gtctgcgcag gcggaacagc cgctgccgca gattgttgtg cgcgcggtcg tcgtccgtat
     1201 cgggccacag cagcgccgcg gccttgtggc gcggcacttt ccgctcgatc gccagcaacg
     1261 ccagcagcgc agcgtcctta cgctcgagca catgaatgcg gcccgtcgca agcaacaggc
     1321 agggcgtgtt cagaagctgc agacgcagcc ccgccgcaga cggtgtcgca tgggtctcac
     1381 cctcggttcg ggtggagccc gccctggtca tcgacccctc cgcagaccgc agccgtcgcc
     1441 tcatgccggt gttggcacgg agtgcaccgg gcggggtaag acgatcatac gcagacgccg
     1501 tggcaagggt gagcgatctt gcggatctgc tgcccaccac cgaagttcaa cgacaaacgc
     1561 gcgaccgcgg tgttctttgg gaatcgcggc ctggccgcgg catcgccggt tcgctgcttg
     1621 cggg
//
LOCUS       DBNHBY010000584         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_243218, whole genome shotgun sequence.
ACCESSION   DBNHBY010000584 DBNHBY010000000
VERSION     DBNHBY010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_243218"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1291
                     /locus_tag="AC16HB_14205"
                     /pseudo
     CDS             112..1291
                     /locus_tag="AC16HB_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
     gene            1709..2218
                     /locus_tag="AC16HB_14210"
     CDS             1709..2218
                     /locus_tag="AC16HB_14210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5607986.1"
                     /translation="MWHVLKQIARTGIRTEAAPAPAGDVAVARLHENVLRTLGRALAI
                     RHVDAGSCNGCELEIHALGNPYYNIEGLSIRFVASPRHADLLLVTGPVSKHMAIALKR
                     TYDATPEPKLVVAAGDCAAGGGVFGESYATLGRVANVIPVDVEVAGCPPTPTAPLEGV
                     LAAVSARRR"
     gene            2268..>2560
                     /locus_tag="AC16HB_14215"
     CDS             2268..>2560
                     /locus_tag="AC16HB_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5607987.1"
                     /translation="MTRDSCDTLVLFGVTGDLAGKMLLPAVHTLIARNALDVPVVGVA
                     RDGGGEPIAQRVRDNVEQDAVLGAAASERLAARLRYVEGDLAAPATFAELRRVL"
BASE COUNT          441 a          799 c          905 g          415 t
ORIGIN      
        1 ttgtcgtccc cgcgcaggcg gggacccaga taccatgaag gactgttcca agttggctcc
       61 cttgagttag aacgagagtg cgaaacactc gctaacgaaa ggagaccagc gatgaaggcc
      121 cctgaaacag cccttcaagc caaggatacg gtgacgcggt cggtgctgta catggctttc
      181 gaactcggcg atcggaagtg gaagcttgct cttggggatg gcacgcgggc gccgagtcga
      241 tatgtgatcg atgcggcgga tctggcggtg ctgatgaaat gcgtggccgg tgccaaggcg
      301 cgctgcggcc tggcgcagac ggtggccatt cgcagttgct acgaggccgg gcgcgacggc
      361 ttctggctgc accgctcggt tgctcgagca cggtatcgac aacgtggtgg tcgactcggc
      421 cagcatcgag gtcaatcgcc gggcgcggcg agccaagagc gatcggctcg acgccgacaa
      481 gctgctgacc atgctgatgc gctatcacgg cggcgagcgg cggctgtggt cggtgctgcg
      541 ggtgcccagt gtggagcagg aggacgggcg gcggctgcac cgcgagctgg atcggttgac
      601 cactgagcgc acggcccaca gcaatcgcat ccgctcgctg ctggtattgc atgggcgaag
      661 ggtgccctcg gtcggcggcc gggactggca acgctggtgg gcggcgcacg ccaggcactt
      721 gccggcggcg atgggagccg agatcgaacg cgaggtcgca cgactggact tggtgcaggt
      781 gcaaatcaag acgctgcacc agatgcagaa cgtacaggtc aaggcgcaga tcgaagcgtg
      841 ccgagagcag ccgagccggc cgccggccaa ccggattgcg cggcaggtga tgcagttgtc
      901 ccggctgcgc gggatcgggg tgatcggtgg ctgggtgttg agcggggaaa tgctgagctg
      961 gcgtcgcttt agcaatcgcc gccaggtggc cgcttgcgtg gggctgaccg gcacgccctt
     1021 tgatagtggc gagagtcggg tcgaccaagg catcagcaag accgcccacc gacagctgcg
     1081 ccgagtgctg gtgcagcttg cctgggattg gcggcgctac cagccgggca gtgatttgag
     1141 tcgctggttc aacgagcgct ttgccggcag tgcgcgtctg cgtcggattg ggatcgtggc
     1201 ggtggctcgg cggctgatca tcgcgctgtg gcgctacatt gaaagcggtg tcgttcccgg
     1261 cggcgccgaa ttcaagcgtg cggtgctgtg aagacgatcc ggctccgaca gcaggggtcg
     1321 aacgcggcat cggatgaaca aggtcaacgc gtccgcaaac gacacgggtc ggccgcggcg
     1381 ccgaccgttc tcccagatgg gacgcgctga gcatcgggtt agaccagcac tttgcgcgta
     1441 tagggcatat ggtgaccgac gtcgtgtcgg cacggataga agttggaccg agcactggac
     1501 tcggcatctt caaacccggc cttgatcctc ggatgcgaat cgaacgaacc aatccacgga
     1561 gccagatcat cgacacacca aaacccgagg gggttgacaa cagtcctcat agaagtgtcg
     1621 ttccactgaa agacgctggg ctcccgcctt cgccggagcg acggcggtcg ttcgccgggg
     1681 tcgaccgagt acacgaggct ccttagacat gtggcacgtg ctcaagcaga tcgcgcgcac
     1741 cggcatcagg acggaagcgg cgccggcgcc cgcgggcgat gttgccgtcg cgaggttgca
     1801 cgagaacgtc cttcgcacgc tcgggcgcgc gctcgcgatc cgccacgtcg acgcgggctc
     1861 gtgcaacggc tgcgagctcg agatccacgc gctcggcaac ccgtactaca acatcgaggg
     1921 actcagcatc cggttcgtcg cgagccctcg ccacgccgac ctgctgctcg tcaccggccc
     1981 cgtgtccaag cacatggcga tcgcgctcaa gcgcacgtac gatgcgacgc ccgagccgaa
     2041 gctcgtcgtc gccgcaggcg attgcgctgc cggcggcggc gtcttcggcg agagctacgc
     2101 gacgctcggc cgcgtcgcca atgtgatccc ggtcgacgtc gaggttgccg gctgcccgcc
     2161 gacgccgacc gcgccgctcg aaggcgttct cgccgccgtg tcggcaaggc gccgctgacc
     2221 ggaacctttc gcggcgcacg gcgacgaacg attgtcgttg cgccgccatg acacgcgatt
     2281 cctgcgacac gctcgtgctg ttcggcgtga ccggcgatct cgccggcaag atgctgctgc
     2341 ccgccgtaca cacgctgatt gcccgcaacg cgctcgatgt gccggtcgtc ggcgtcgccc
     2401 gcgacggcgg cggcgaacct atcgcgcagc gggtgcgcga caacgtcgag caggatgcag
     2461 tgctcggcgc cgccgcaagc gagcggctcg ctgcgcgcct gcgctacgtc gaaggcgatc
     2521 tcgcggctcc ggcgacgttc gccgaattgc gccgtgtact
//
LOCUS       DBNHBY010000585         2760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1054082, whole genome shotgun sequence.
ACCESSION   DBNHBY010000585 DBNHBY010000000
VERSION     DBNHBY010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1054082"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="AC16HB_14220"
     CDS             <1..692
                     /locus_tag="AC16HB_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5607988.1"
                     /translation="DAQARALVMWGAGGHFSAGADFDRFQQLMTQSPPAGEADPIATH
                     NRAFGAALERIARLPVPTLAVVRGAAMGGGCGLACVTDRVIAADDAVFALPEVALGVA
                     PAQIAPFVARRVGAQRARWLMLAAARLDAAAALACGLADVIAPVAGLGAAVARELQGL
                     AAAEPAALRATRRIALSCAEQPLATVLDGAALEFAALLRGGAAREGIAATRERRRPAW
                     QQPVPDLPEFA"
     gene            689..2692
                     /locus_tag="AC16HB_14225"
     CDS             689..2692
                     /locus_tag="AC16HB_14225"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR5607989.1"
                     /translation="MRPLRRLLIANRGEIVVRVARTARRLGIETIAVYSDADAGSPHV
                     LACDAAASLGGLAAADSYLRVDKLLAAARDLRADAIHPGYGFLAENAAFAAAVAEAGL
                     IFVGPPATAIEAMGDKARARQRMAAAGIPVVPGYDGEAQDAATFAAQAARIGYPVMIK
                     AAAGGGGRGMRLVGSAEELPDALRSAALEAAKSFGDGRLILERAVAEPRHVEIQVFAD
                     TQGNVLHLGERDCSVQRRHQKVLEESPSPAVDDELRARMGATAVAVAREIGYVGAGTV
                     EFLLDPAGHYYFMEMNTRLQVEHPVTELVTGLDLVELQLRVARGEALPLRQADVRLQG
                     HAIEARLCAESPVDDFLPGSGLVLDWEAPAHVRCDHALASGGMVPAWYDSMVAKVIAH
                     GATRAESVADLAAALDHTVLLGLPSNRAFVASMLRHPVFAEGRDVSTAFIGRHFAEPA
                     SRAIRPDARTWALAAWLSVAAAPEAWCTPAAWRNFSTGRPLPQPWRLRWLAPAALASP
                     DALQDGRLRLDGNRGFVEAGAARHEIEGLPAAPRTPGIARIDAVALDYRYAWDGGTLW
                     LHTAQGDFAFASLRREPARSAQAEAAAAAEVRAVINARVVDVRAVVGAEVNAGDRLVV
                     LEAMKMEHEVRAQRAGRVAEVSVKPGDQVAPGQLLVRYEETAR"
BASE COUNT          362 a          979 c         1021 g          398 t
ORIGIN      
        1 ccgatgcgca ggcccgtgcg ctcgtgatgt ggggtgcggg cgggcatttc agcgccggcg
       61 cggacttcga ccgcttccag cagctgatga cgcaaagccc gccggccggt gaagccgacc
      121 cgattgccac gcacaaccgt gcgttcggcg cggcgctgga gcgcatcgcg cgccttccgg
      181 tgccgacgct cgcggtggtg cggggagccg cgatgggcgg cgggtgcggg ctggcttgcg
      241 tcaccgaccg ggtgatcgca gcggacgatg ccgtgttcgc attgcctgaa gtcgcgctcg
      301 gcgtcgctcc ggcccagatc gcgccgttcg tcgcgcgtcg agtcggcgcg cagcgcgcgc
      361 gctggctgat gctcgctgcc gcgcggctcg acgcagcggc agcgctcgcc tgcggcctgg
      421 cggatgtcat tgctccagtg gccgggctgg gagcggccgt cgctcgcgag ctgcagggtc
      481 tcgctgccgc agagcctgct gcgctgcggg caaccaggcg catcgccctc agctgcgccg
      541 agcagccgct ggccacagtg ctcgatgggg ctgccctcga attcgctgcg ctgctgcgcg
      601 gcggcgccgc acgcgaaggc atcgcagcca cgcgcgagcg gcgtcgtccc gcctggcagc
      661 aaccggtgcc cgacctgccg gagttcgcat gagaccgctg cgccgcctgc tgatcgccaa
      721 ccgcggcgag atcgtcgtcc gcgtcgcgcg caccgcgcgg cgcctgggca tcgagacgat
      781 tgcggtctac tccgacgcgg atgcgggcag tccgcacgtg ctcgcctgcg atgccgccgc
      841 gtcactcggc ggcctcgccg ccgcggacag ctacctgcgc gtcgacaagc tgctcgcggc
      901 cgcgcgcgac ctgcgcgccg atgccatcca tcccggctat ggattcctcg cggagaacgc
      961 agccttcgcc gcggctgtcg ccgaggcggg cctgatcttt gtcgggccgc ccgcaaccgc
     1021 gatcgaggcg atgggcgaca aggcgcgtgc gcgccagcgt atggccgcag caggaattcc
     1081 cgttgtgccg ggttatgacg gcgaggcgca ggacgccgcg acattcgcgg cccaggcagc
     1141 ccgcatcggc tacccggtga tgatcaaggc ggcggccggt ggcggcggcc gcggcatgcg
     1201 cctggtgggc agcgccgaag aactgccgga tgcgctgcgc agcgcggcgc tcgaggcagc
     1261 gaaatcgttc ggcgacggcc ggctgatcct cgagcgtgcc gtggccgagc cgcgccacgt
     1321 cgagatccag gtgttcgccg acacgcaggg caacgtgctg catctcggcg agcgcgactg
     1381 ctccgtgcag cggcggcacc agaaggtgct cgaagagtcg ccctcgcccg ccgtcgacga
     1441 cgaactgcgc gcgcgaatgg gcgcgacagc ggtcgcggtg gcacgcgaga tcggttacgt
     1501 cggcgccggc acggtcgagt tcctgctcga tcccgccggg cactattact tcatggaaat
     1561 gaacacgcgg ttgcaggtcg agcacccggt cacggagctc gtgacgggac tcgacctggt
     1621 cgagctgcag ctgcgggtcg cgcgcggcga agcgctgccg ctgcgacagg ccgacgtccg
     1681 attgcagggt cacgcgatcg aagcgcgcct ctgtgccgaa tcgccggttg acgacttcct
     1741 gccgggcagc ggcctcgtgc tcgactggga ggctcccgcg cacgtgcgct gcgatcacgc
     1801 gctcgccagt ggtggcatgg tgcccgcgtg gtacgactcg atggtcgcga aggtcatcgc
     1861 gcacggggcg acccgtgctg aaagcgtcgc ggacctcgcc gccgcgctcg atcacacggt
     1921 actgctcggc ctgccgagca accgcgcgtt cgtcgcgagc atgctgcggc atcccgtgtt
     1981 cgccgaaggc cgcgacgtca gcaccgcgtt catcggccgt catttcgccg agcccgccag
     2041 ccgggcgatc cggcccgacg cgcgtacctg ggcgctggcc gcatggctgt cggtcgcggc
     2101 tgcgccggag gcgtggtgca cgcccgctgc gtggcgcaat ttctcgacgg ggcgccccct
     2161 gccccagccc tggcgactgc gctggctggc ccccgccgcg ctggcgtcgc cggatgcgct
     2221 gcaggacggg cgcttgcggc tggacggcaa ccgcggcttc gtcgaagccg gcgccgcccg
     2281 gcacgagatc gagggactgc cagccgcgcc gcgcactccg ggtatcgcgc gcatcgacgc
     2341 cgttgcgctc gactatcgat acgcatggga cggcggcacc ttgtggctgc acacagcgca
     2401 aggcgacttc gctttcgcca gcctgcggcg cgagccggcg cgttccgccc aggccgaggc
     2461 cgccgcagcc gcggaagtcc gcgccgtgat caatgcgcgc gtcgtcgacg tgcgggccgt
     2521 cgtcggcgcg gaagtgaatg cgggcgaccg cctcgtcgtg ctcgaggcca tgaagatgga
     2581 gcacgaggtc cgggcgcagc gagctggtcg cgtcgccgaa gtttccgtca agccgggcga
     2641 ccaggtggcg ccgggtcaac tgctggtgcg atacgaggag accgcgcgat gaacgacaag
     2701 gtcgtgctga cgtgcgcgct gaccggcgtg ctgaccgacc cggcccagca tccggtgccg
//
LOCUS       DBNHBY010000586         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_648241, whole genome shotgun sequence.
ACCESSION   DBNHBY010000586 DBNHBY010000000
VERSION     DBNHBY010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_648241"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="AC16HB_14230"
     CDS             <1..295
                     /locus_tag="AC16HB_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR5607990.1"
                     /translation="NQMYSQRYGTVPIVGATGGLLDSVVDCSDATLADGTASGFHVSP
                     LDIAGLRSAVARALALYREPQRWRRLQRAGMARDFSWKRAAVEYARLYNQIAR"
     gene            complement(299..>2175)
                     /gene="glgP"
                     /locus_tag="AC16HB_14235"
     CDS             complement(299..>2175)
                     /gene="glgP"
                     /locus_tag="AC16HB_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5607991.1"
                     /translation="ENSDHDRFIAHRLYGGDRGTRIEQEIVLGMGGVRALAALNLRPT
                     VWHINEGHAAFLVLERVRERMKEGLEFAAAIEAVAASTVFTTHTAVPAGHDHFAEDMI
                     RRYFDGFCRDQGVDVQALMALGRTPASPDFNMTALAVRGSRHRNGVSRIHGRVSSQML
                     RELWPQVPPAENPIAYVTNGVHAPTFLASEWIDIFDRFLGPDWMQRLSDPQCWQRVHE
                     IPDHIYWSVRQYLKAQMLQLVAYRIRHQVRRAHGSEAHADRLLRRADPADPNVLTIGF
                     ARRFATYKRATLMFEQLDLLDELVNDPQRPLLFIFAGKAHPADTPGQDLMRRIAEIAK
                     MPRFEGKILFVEGYDLRLARRLTSGVDVWLNHPTYPLEACGTSGMKAAMNGVLNLSIR
                     DGWWDEGYAAGNGWAITPIPEDRSQDARNRGEVRSIYEVLQDQVIPLYYDRGPMGFSP
                     GWVTMGKEAIATLMPRFNTERMLTEYVTRFYRPAAELGRRVAAEGYAQARELARWKER
                     IYARWPGVKVRAVEAVPRRIAYGEGMHLAIDVALNGLKADDVVVEALLADGSHASAAN
                     DAKSLSLSPTGAPDGSDLQRYELKFAPERCGRFEYRIRAYPRHPLLAHPFELGLMLWV
                     "
BASE COUNT          343 a          742 c          742 g          348 t
ORIGIN      
        1 caatcagatg tacagccagc gctacggcac ggtgccgatc gtcggtgcca cgggcgggct
       61 gctcgactcg gtcgtcgatt gcagcgacgc gacgctggcc gatggcacgg caagcggatt
      121 ccacgtcagt ccgctcgata tcgccggcct gcgcagcgcg gtggcgcgtg cgctcgcgct
      181 gtaccgcgaa ccgcagcgct ggcggcgcct gcagcgggcc ggcatggcgc gcgacttcag
      241 ctggaagcgc gcggcggtcg agtacgcgcg gctgtacaac cagatcgcac gctaggcgct
      301 agacccagag catcaatccg agttcgaacg ggtgcgcgag cagcgggtgg cgcggatacg
      361 cgcggatccg gtattcgaag cggccgcagc gctcgggcgc gaacttcagt tcgtagcgct
      421 gcaggtccga cccgtccggc gcaccggtgg gcgacagcga cagcgatttg gcgtcgttcg
      481 cggccgacgc gtgcgagccg tccgccagca gcgcttcgac caccacgtcg tcggccttca
      541 gtccattcag cgccacgtcg atcgccagat gcatgccctc gccgtacgcg atgcggcgcg
      601 gcactgcttc gaccgcgcgc accttgacgc caggccagcg cgcatagatg cgctccttcc
      661 agcgcgccag ttcgcgtgcc tgcgcatagc cttcggcggc aacgcgacgg ccgagttcgg
      721 ccgccggccg gtagaagcga gtcacgtact cggtcagcat gcgctcggtg ttgaagcgcg
      781 gcatcagcgt ggcgatcgct tccttgccca tcgtcaccca gcccggcgaa aagcccatcg
      841 ggccgcggtc gtagtagaga gggatgacct ggtcctgcag gacctcgtag atcgagcgga
      901 cctcgccgcg attgcgcgcg tcctggctcc ggtcctcggg aatcggcgtg atcgcccagc
      961 cgttgcccgc tgcgtagccc tcgtcccacc agccgtcacg gatcgacagg ttcagcacgc
     1021 cgttcatcgc cgccttcatg cccgaggtgc cgcacgcctc gagcggatag gtcggatgat
     1081 tcagccacac gtccacgccc gacgtcagcc gtcgcgccag ccgcaggtcg tagccttcga
     1141 cgaacaggat cttgccctcg aagcgtggca tcttcgcgat ctcggcgatg cggcgcatca
     1201 ggtcctggcc cggcgtgtcc gccgggtgtg ccttgccggc gaagatgaac agcagcggcc
     1261 gctgcggatc gttgacgagt tcgtcgagca ggtcgagctg ctcgaacatc agcgtggcgc
     1321 gcttgtacgt ggcgaagcgg cgcgcgaagc cgatcgtcag cacgttggga tcggcgggat
     1381 cggcgcggcg caataggcga tcggcgtgcg cctcgctgcc gtgcgcgcgc cgcacctgat
     1441 gccggatccg gtacgcgacc agctgcagca tctgcgcctt caggtactgc cgcacgctcc
     1501 agtagatgtg atccggaatc tcgtgcacac gctgccagca ctggggatcg gacagccgct
     1561 gcatccagtc ggggccgaga aagcggtcga agatgtcgat ccactcggac gcgagaaagg
     1621 ttggcgcgtg cacgccgttg gtcacgtacg cgatcgggtt ctcggccggc ggcacctgcg
     1681 gccacaactc gcgcagcatc tgcgagctaa cgcgcccgtg gatgcggctg acgccgttgc
     1741 ggtggcgcga gccgcgcacc gcgagcgcgg tcatgttgaa gtcggggctg gccggcgtgc
     1801 gacccagtgc catcaacgcc tggacgtcga ctccctgatc gcggcagaag ccgtcgaaat
     1861 agcgccggat catgtcctcg gcgaaatgat catgccccgc cggcacggcg gtatgcgtgg
     1921 tgaagaccgt gctggcggcc accgcctcga tcgcggccgc gaattcaagg ccctccttca
     1981 tgcgctcgcg cacgcgctcg agcacgagga atgcggcatg tccttcgttg atgtgccaga
     2041 ccgtggggcg aagattcagc gccgcgagcg cgcgcacgcc gcccatgccg agcacgatct
     2101 cctgctcgat gcgcgtgccg cgatcgccgc cgtacaaccg atgcgcgatg aagcgatcgt
     2161 ggtcgctgtt ctccg
//
LOCUS       DBNHBY010000587         2347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1054259, whole genome shotgun sequence.
ACCESSION   DBNHBY010000587 DBNHBY010000000
VERSION     DBNHBY010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1054259"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..663)
                     /locus_tag="AC16HB_14240"
     CDS             complement(187..663)
                     /locus_tag="AC16HB_14240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0047173 - phosphatidylcholine-retinol
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0042572 - retinol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HIR5607992.1"
                     /translation="MTHPLRSAPSQVDCDLPIGAHLVTPRLGFTHHGIHVGDGRVVHY
                     AGFSRALLLRGPVEEVTLAHFAGGRAVEVKTHPRARFAPDEVVARARSRLGEDRYRLT
                     TNNCEHFCEWCLSGESRSEQVDSLFSRMRDAARVVGQFVREAFGTEPGDGFGVSKT"
     gene            complement(918..1628)
                     /locus_tag="AC16HB_14245"
     CDS             complement(918..1628)
                     /locus_tag="AC16HB_14245"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR5607993.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEDAGADYVDVARERRKGMGMAA
                     MLTLMDSAARPPFAPPFLKAGRVLIAQTANILFYLGPRLALAPKSESDRLWAHQLQLT
                     IADCVAEAHDVHHPIAGSLYYDDQKAEALRAARHFTRERMPKYLGYFERVLARKPWIL
                     GRQATYVDLSLFQLLAGLRYAFPRAMKKCARKTPRLATLAERVAERPRLAAYLSSPRR
                     LPGNEQDVFRHYPELDAD"
     gene            1639..1866
                     /locus_tag="AC16HB_14250"
     CDS             1639..1866
                     /locus_tag="AC16HB_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607994.1"
                     /translation="MTIRVECYAGYRGDQEPLAFWLGARRIGVRAVIDRWFAPDQRWF
                     KVDGDDGHAYVLRHDEAEGTWELAAFTRGAG"
     gene            complement(1847..>2347)
                     /locus_tag="AC16HB_14255"
     CDS             complement(1847..>2347)
                     /locus_tag="AC16HB_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5607995.1"
                     /translation="PWGALAFGAGLLGLSVWLLKQDIARRTVRKSGLTRFIAVCLLSG
                     YVWLAAGGAIVLAAGVLAPGTPAYDAALHALALGFVFSMVFGHAPIIFPAVLRVDVPY
                     HPTFYVPLALLHLSVALRLAGDAVGHFGAVRVGGLLGAFALLAFIVSTIAAVVRGRRA
                     VSLRRA"
BASE COUNT          372 a          831 c          822 g          322 t
ORIGIN      
        1 cttgccgccg cgctgctgcg gctggccgcc aacgtgcacg ggttcggcac gtgggccatc
       61 gcgctggccg cggtgctgtg ggcggcgccg ttcgcgatct acctgctgct gtacggcgcc
      121 acgctgctgc agccgagcct tccgcgcgca cagccgctgc agccgacctg accggttcgg
      181 gcggactcag gtcttgctca cgccgaagcc gtcgcccggt tcggtgccga acgcttctcg
      241 gacgaactga ccgacgacgc gcgcggcatc gcgcatgcgg ctgaacaggc tatcgacctg
      301 ctcgctgcgg ctttcgcccg acaggcacca ctcgcagaag tgctcgcagt tgttggtcgt
      361 caaccggtac cgatcctcgc cgaggcgcga gcgcgccctg gcgacgactt cgtcgggcgc
      421 gaagcgggcg cgcggatgcg tcttcacctc gaccgcgcga ccgcccgcga agtgcgccag
      481 cgtgacttcc tccaccggcc cgcgcagcag cagcgcacgc gaaaatccgg cgtagtgcac
      541 gacccggccg tcgccgacgt ggattccgtg atgggtgaat cccaggcgcg gcgtcaccag
      601 gtgagcgccg atcggcaggt cgcaatccac ctgcgacggc gcggagcgga gcggatgggt
      661 cattttcagg tcctcgcatc ggattcatgc cacgccccga ttgaatcggg attgcggccg
      721 accgagcagt cgggacgtgt cccagcatcg gtgtgctccg agctagggat ggacctaacg
      781 gggacccgag acgcgatgga cgccgcctcg gcgccgcgct ggctgcgccg gaacggatcc
      841 cgcccggtac gacgcgacgc gcgtgcccgt gcgcagcacg agcccgtcac gggcccggcg
      901 gcggcgcgtg acgcagttca gtcggcgtcg agctcggggt agtgccgaaa cacgtcctgc
      961 tcgttgccgg gcagtcggcg cggcgaggac aggtaggcgg cgagccgcgg ccgctcggcg
     1021 acgcgctcgg ccaatgttgc gagccgcggc gtcttccgtg cgcacttctt catcgcgcgc
     1081 ggaaacgcgt agcgcagtcc ggccagaagc tggaacagcg acaggtcgac gtaggtggcc
     1141 tgccggccga ggatccacgg cttgcgcgcc agcacgcgct cgaagtatcc gaggtacttc
     1201 ggcatccgct cgcgcgtgaa gtggcgcgcg gccctgagcg cctcggcttt ctggtcgtcg
     1261 tagtacaggc tgcccgcgat cggatggtgc acgtcgtgcg cctcggccac gcagtcggcg
     1321 atcgtcagtt gcagttggtg cgcccacagc cggtcggact cgctcttcgg cgcgagcgcc
     1381 agtcgcgggc cgagatagaa aagaatgttg gcggtctgtg cgatcagtac gcggcccgcc
     1441 ttcaggaagg gcggcgcgaa cggcggccgc gccgcgctgt ccatcagcgt cagcatcgcg
     1501 gccatcccca tgcccttgcg ccgctcgcgc gcaacgtcga cgtagtcggc gccggcgtcc
     1561 tcgagcgcca gccgcacgaa ttcaccgcgg ccctggatcg acggccagta atacagttca
     1621 taacgcatcg gaacccccat gacgattcgc gtcgagtgct acgccggcta tcgcggcgac
     1681 caggaaccgc tcgcattctg gctcggcgcg cggcggatcg gcgtgcgcgc agtgatcgat
     1741 cgctggttcg cgccggatca acgctggttc aaggtcgacg gcgacgacgg ccacgcctac
     1801 gtgctgcggc acgacgaggc ggagggcacc tgggagctgg cggcgttcac gcgcggcgca
     1861 ggctgaccgc gcgcctgccg cgcacgaccg ccgcgatcgt gctgacgatg aaggcgagca
     1921 gcgcgaacgc gcccagcagg ccccccacgc ggaccgcgcc gaagtgtccg accgcatcgc
     1981 cggccagccg cagggccacc gacagatgca gcaatgcgag tgggacgtag aacgtcgggt
     2041 gatacggcac gtcgacgcgc agcaccgccg ggaagatgat cggcgcgtgg ccgaagacca
     2101 tcgagaacac gaatcccaac gccagcgcgt gcagcgctgc gtcgtacgcc ggcgtgccgg
     2161 gcgccaggac accagcagcc agcacgatcg cgccgcccgc ggcgagccac acgtagcccg
     2221 aaagcagaca caccgcgatg aagcgcgtca gcccgctctt gcgcaccgtg cgacgcgcga
     2281 tgtcctgctt cagcagccag accgacagcc cgagcagccc ggcaccgaag gcgagcgcgc
     2341 cccacgg
//
LOCUS       DBNHBY010000588         5442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_311312, whole genome shotgun sequence.
ACCESSION   DBNHBY010000588 DBNHBY010000000
VERSION     DBNHBY010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_311312"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="AC16HB_14260"
     CDS             <1..1161
                     /locus_tag="AC16HB_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5607996.1"
                     /translation="GTNTMNPRAKALLASSIGVRPSLGHPGEKYNKGVHEIERIEVLA
                     LELLKQLFNAPYAEYRVPSGSIANLYAFMATCRPGDTIIAFDDAAAGHPTHHAEGAAG
                     LYGLKVLAMPFDAARMDVDVDELRRLARDARPKLVIVAGSMCLFPYDVRAVRGIADEV
                     GAWVMYDAAHMGGLIAGGAFQQPLAEGAHLMTGSTYKSFGGPPSGMVLTTEAALAERL
                     DRIAYPGLTANFDAAKTAALALATMDLIAHGRAYARQMVANAQALAEALAAEGVAVFK
                     PAGRDGFTVSQHVALDARPCGGGNRASAHVERANILFTSIGLPLPAVEGDANGIRIGT
                     QEVTRWGLREDAMRDVARLIARVLVRGEAPERVRADAVALRSQYQQLQFVRA"
     gene            1224..1949
                     /gene="folE"
                     /locus_tag="AC16HB_14265"
     CDS             1224..1949
                     /gene="folE"
                     /locus_tag="AC16HB_14265"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HIR5607997.1"
                     /translation="MDDVTRPTKPQADDLGVPVSQKIRERIERARERYHANDNISRFI
                     EPDELDRLLDEVEEKMRGVLRSLVIDVDSDHNTQDTARRVAKMYLTEVFRGRYQPAPS
                     VTEFPNVEHLNELMIVGPIMIRSACSHHLCPIIGKLWIGVMPNEHSNLIGLSKYVRLA
                     DWVMSRPQIQEEAVTRLADLLQERMQPDGLAIVMEADHFCMQWRGVKDTDSKMINSVM
                     RGSFLVNTSLRREFLALLQRRTA"
     gene            1960..2388
                     /locus_tag="AC16HB_14270"
     CDS             1960..2388
                     /locus_tag="AC16HB_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HIR5607998.1"
                     /translation="MLIRLLYASRSAETVTPGMIDSILASSRRRNPELGITGILCHSG
                     DIFMQVLEGGRAPVNELYNSIVRDPRHKDVTILQCVEVSERRFAGWTMGQVNLARVNA
                     STLLKYSDRPGLDPYALPGRVAMALLEELIATAQIVGRAC"
     gene            2398..2871
                     /locus_tag="AC16HB_14275"
     CDS             2398..2871
                     /locus_tag="AC16HB_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5607999.1"
                     /translation="MPHDPDWRHHGVRVIKASTLDPNTAQTPGMDRKAAINYARVGAQ
                     KIWAGTVTIHANAKTGAHHHGALESVIYVVRGKARMRWGERLEFVAEADAGDFIYVPP
                     YVPHQEINASTTETLECVLVRSDNEAVVVNLDIADVEANPEAVYWVDPIHQAPRG"
     gene            2903..3826
                     /gene="cysK"
                     /locus_tag="AC16HB_14280"
     CDS             2903..3826
                     /gene="cysK"
                     /locus_tag="AC16HB_14280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR5608000.1"
                     /translation="MKASTILDTIGGTPHIRINRLFGHTHEVWIKSERANPGGSIKDR
                     IALSMVEDAERTGKLAPGGTIIEPTSGNTGIGLAMVAAVKGYRLILVMPESMSIERRR
                     LMLAYGATFELTPREKGMPGAIARATELLAATPGAWMPQQFDNPANVDVHERVTAREI
                     LEDFADQPLDVLITGVGTGGHITGCALTLKRAWTKLKVYAVEPAASPVISGGKPSPHP
                     IQGIGAGFIPKNLNVELLDGVIQVDAEPAREMARRAAREEGMLVGISSGATLAAIAQK
                     LPELPPGARVLGFNYDTGERYLSTEGFLPAA"
     gene            complement(3845..4120)
                     /locus_tag="AC16HB_14285"
     CDS             complement(3845..4120)
                     /locus_tag="AC16HB_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608001.1"
                     /translation="MRIRPTILALGIAFTALSAQAQTAPQPARPVQEGPSSVFVYEKL
                     GATPSLQRPHALHLTRAAQTRSGGEGEAAATMQVSKSPTPTAARSVN"
     gene            4231..5217
                     /locus_tag="AC16HB_14290"
     CDS             4231..5217
                     /locus_tag="AC16HB_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5608002.1"
                     /translation="MSIRSEYPAGDAGAPPAFDLLTDVMEQVRLEGTVFFTAELHAPY
                     GIAINRPHRSPFYIALEGECEIQVGGKVHRVGPRDFVLLPKAAPHVVRSDPKARIVPF
                     DDWLATHPLDAHGFTLHRGPGTARRVMGGFFSTDALKVNPLFDALPPYIQLRGSDPDV
                     RRWLAPTVDFIRAEIDSGLQGSRTILRRMADVLFIQALRAYAARNRAATGWLRGLGDP
                     RVAKALVLMHQRYAEPWTLDSLARAAGTSRTALAVQFRRLVGEAPMAYLARWRITRVA
                     NRLRSERISLTRVAESVGYQSDAVFSKAFLRVTGVSPGRYRRAAAEAAAAPG"
     gene            5262..>5442
                     /locus_tag="AC16HB_14295"
     CDS             5262..>5442
                     /locus_tag="AC16HB_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR5608003.1"
                     /translation="MSLPALLRDRLRLPVIGAPLFIVSNPTLVIEQCKAGIVGAFPAL
                     NARPAHVLDEWLVRIK"
BASE COUNT          891 a         1868 c         1873 g          810 t
ORIGIN      
        1 ggcaccaaca cgatgaaccc gcgcgccaag gcgctgctgg cgtcgtcgat cggcgtgcgg
       61 ccgagcctcg gccaccccgg cgagaagtac aacaagggcg tgcacgagat cgagcgcatc
      121 gaggtgctgg cgctcgaact gctgaagcaa ctcttcaacg cgccgtatgc cgagtaccgc
      181 gtgccgagcg gctcgatcgc caacctgtac gccttcatgg cgacctgccg gccgggcgac
      241 acgatcatcg cgttcgacga cgccgcggcg gggcacccga cccatcacgc ggagggcgcg
      301 gcggggctgt acggcctgaa ggtactcgcg atgcccttcg acgctgcgcg catggacgtc
      361 gatgtcgacg agctgcggcg gctcgcgcgc gacgccagac cgaagctcgt catcgttgcg
      421 ggatcgatgt gcctcttccc gtacgacgtg cgcgcggtgc gcggcatcgc cgacgaggtc
      481 ggcgcatggg tgatgtacga cgccgcgcac atgggcgggc tgattgcggg cggcgcattc
      541 cagcagccgc tcgccgaggg tgcgcacctg atgacgggca gcacgtacaa gagcttcggc
      601 ggcccgccga gcggcatggt gctgacgacc gaggcggcgt tggcggagcg gctcgaccga
      661 atcgcgtacc ccggcctgac cgcgaacttc gacgccgcca agaccgccgc gctggcgctg
      721 gcgacgatgg atttgatcgc gcacggccgc gcctacgcgc gccagatggt cgccaacgcg
      781 caggcgctcg ccgaggcgct cgccgcagag ggcgtggcgg tgttcaagcc ggccggacgc
      841 gacgggttca cggtgtcgca gcacgtggca ctcgatgcgc ggccctgtgg cggcggcaat
      901 cgcgcgtccg cacacgtcga aagggcgaac atcctgttca cgtcgatcgg cctgccgctc
      961 cccgcggtcg aaggcgacgc caacggcatc cgcatcggca cgcaggaggt cacgcgctgg
     1021 ggcctgcgcg aggacgcgat gcgcgatgtg gcgcggctga tcgcgcgcgt gctggtgcgc
     1081 ggcgaggcgc ccgagcgcgt gcgcgcggat gcggtcgcgc tcaggtcgca gtaccagcag
     1141 ctgcaattcg tccgggcata gctccccggc gccgccgcgc gttgcgggta cgatgcagat
     1201 ccgaacctga cggggtctgc ccgatggacg atgtcacccg ccccacgaaa ccgcaggccg
     1261 acgatctcgg cgttccggtt tcgcagaaga ttcgcgagcg aatcgaacgc gcgcgcgagc
     1321 gctaccacgc caacgacaac atctcccgct tcatcgagcc ggacgaactc gaccgcctgc
     1381 tggacgaggt cgaggaaaag atgcgcggcg tactgcggag cctggtgatc gatgtcgaca
     1441 gcgatcacaa cacgcaggac accgcgcgcc gcgtggcgaa gatgtacctg accgaggtgt
     1501 tccgcggccg ctaccagccg gcgccgtcag tgaccgagtt cccgaacgtc gagcacctga
     1561 acgagctgat gatcgtcgga ccgatcatga tccgcagcgc gtgctcgcac cacctgtgtc
     1621 cgatcatcgg caagctgtgg atcggcgtga tgccgaacga gcactcgaac ctgatcgggc
     1681 tgtccaagta cgtccggctg gcggactggg tgatgagccg gccgcagatc caggaagagg
     1741 cggtcacgcg gctggcggac ctgctgcagg agcgcatgca gcccgacggg ctggccatcg
     1801 tgatggaagc cgatcacttc tgcatgcaat ggcgcggcgt caaggacacc gattcgaaga
     1861 tgatcaacag cgtcatgcgc ggcagcttcc ttgtcaatac cagcctgcgg cgcgaattcc
     1921 tcgcgctgct gcaacgaagg acggcctgag aggacaccca tgctgattcg cctgctctac
     1981 gcgagccgct cggccgagac ggtcacgccg gggatgatcg attcgattct cgcttcctcg
     2041 cgccgccgca acccggaact cggcatcacc ggcatcctgt gccacagcgg cgacatcttc
     2101 atgcaggtgc tcgagggcgg gcgcgcgccg gtcaacgagc tgtacaacag catcgtgcgc
     2161 gatccgcgcc acaaggacgt gaccatcctg cagtgcgtgg aggtgtcgga gcggcgcttt
     2221 gccggatgga ccatgggcca ggtcaatctg gccagggtca acgcatcgac gctgctgaag
     2281 tactccgacc ggccggggct cgacccgtac gcgctgcccg gccgcgtcgc gatggcgctt
     2341 ctggaggagc tgatcgcgac cgcgcagatc gtcggccgcg cttgctagga ggagaacatg
     2401 ccgcatgacc ctgactggcg ccaccacggc gtgcgcgtga tcaaggccag caccctcgat
     2461 cccaataccg cgcagacgcc gggcatggac cgcaaggccg cgatcaacta tgcgcgcgtg
     2521 ggcgcgcaga agatctgggc cggcaccgtg acgatccacg ccaatgccaa gaccggcgcg
     2581 catcaccacg gcgcgctcga aagcgtcatc tacgtcgtac gcggcaaggc gcgcatgcgc
     2641 tggggcgagc ggctggagtt cgtcgccgag gccgacgcgg gcgatttcat ctacgtgccg
     2701 ccgtacgtgc cgcaccagga gatcaacgca tcgacgaccg agacgctcga atgcgtactc
     2761 gtgcgcagcg acaacgaggc ggtggtggtg aacctcgaca tcgccgacgt cgaagcgaac
     2821 cccgaagccg tctactgggt cgatcccatc catcaggcgc cgcgcggctg agcgcgacca
     2881 gcacgcatcc cggagaagat ccgtgaaagc cagcaccatc ctcgacacga tcggcggcac
     2941 gccgcacatt cgcatcaatc gcctgttcgg ccacacgcac gaggtgtgga tcaagtccga
     3001 gcgcgccaac cccggcggtt cgatcaagga tcgcatcgcg ctgtcgatgg tcgaggacgc
     3061 ggagcgcacc ggcaagctcg cgcccggggg cacgatcatc gaacccacgt cgggcaacac
     3121 cggcatcggc ctggcgatgg tggccgccgt caagggctac aggctgatcc tggtgatgcc
     3181 cgagagcatg tcgatcgaac gccgccggct gatgctcgcc tatggcgcga cgttcgagct
     3241 gaccccgcgc gagaagggca tgccgggggc gatcgcgcgc gccaccgaac tgctggcggc
     3301 cacgccgggt gcgtggatgc cgcagcagtt cgacaacccg gccaacgtcg acgtgcacga
     3361 gcgcgtgacc gcacgcgaga tcctggagga cttcgccgat cagccgctcg acgtgctgat
     3421 caccggtgtg ggcaccggcg gccacatcac cggttgcgcg ctgacgctca agcgggcgtg
     3481 gacgaagctg aaggtctacg cggtcgagcc agcggcgtcg ccggtgatct cgggcggcaa
     3541 gccgtcgccg cacccgatcc agggcatcgg cgccggcttc atcccgaaga acctgaacgt
     3601 cgaactgctc gacggcgtga tccaggtcga cgcagagcca gcgcgcgaga tggcgcgccg
     3661 tgccgcgcgc gaggaaggga tgctggtcgg catctcgtcc ggcgccacgc tggcggcgat
     3721 cgcgcagaag ctgcccgaat tgccgcccgg cgcgcgcgtg ctcggcttca actacgacac
     3781 cggcgagcgc tatctgtcga ccgagggctt tctgccggcg gcgtaacgcg ccagccggcg
     3841 cgcctcaatt gacgctgcgc gcggcggtcg gggtggggct cttgctcacc tgcatggtgg
     3901 cggccgcttc tccttcgccg cccgagcgcg tctgcgcggc gcgagtcaga tgcagcgcat
     3961 gcggccgttg cagcgacggc gtggcgccga gtttctcgta gacgaacacg ctggacggac
     4021 cttcctgcac agggcgcgcc ggctgcggtg cggtctgcgc ctgcgcgctc agcgccgtga
     4081 aagcgatgcc aagggcaagg atcgtggggc ggatacgcat cttgagctcc tttcggttgc
     4141 gtcgttcggt gtcgagggac accgttcgtt cgacggttcc cactgtgcgc ctcgcgtgtc
     4201 agaatgaaaa gacacttgcg gacacaattc atgtcgattc gttcagagta ccctgcgggc
     4261 gacgccggcg ccccgcccgc tttcgacctg ctgaccgacg tgatggagca ggtgcgcctc
     4321 gagggcacgg tgttcttcac cgccgagctg cacgcgccgt acggcatcgc gatcaaccgg
     4381 ccgcatcgca gcccgttcta catcgcgctc gaaggcgagt gcgagatcca ggtcggcggc
     4441 aaggtccatc gggtcggccc gcgcgatttc gtgctgctgc ccaaggccgc gccgcacgtc
     4501 gtgcgctcgg acccgaaggc gcgcatcgtg ccattcgacg actggctcgc cacgcacccg
     4561 ctcgatgcgc acggcttcac cctgcatcgc ggcccgggca cggcgcggcg cgtgatgggc
     4621 ggcttcttct caaccgatgc gctcaaggtc aacccgctgt tcgacgcgct gccgccgtac
     4681 atccagctgc gcggatcgga ccccgacgtg cggcgctggc tcgcgcccac ggtcgatttc
     4741 atccgcgccg agatcgactc tggcctgcag ggctcgcgca cgatcctgcg ccgcatggcc
     4801 gacgtgctgt tcatccaggc gctgcgcgca tacgccgcgc gcaaccgtgc cgcgacggga
     4861 tggctgcgcg ggctcgggga cccgcgcgtg gcgaaggcgc tggtgctgat gcaccagcgc
     4921 tatgcggaac cgtggacgct cgactcgctc gcgcgcgcgg ccggcacctc gcgcaccgcg
     4981 ctggcggtgc agttcaggcg gctcgtcggc gaggcaccga tggcgtacct ggcgcggtgg
     5041 cgcatcacgc gcgtggccaa tcggctgcgc agcgagcgca tatcgctgac acgagtagcc
     5101 gagagcgtgg gctaccagtc ggacgccgtg ttctccaagg cattcctgcg cgtgaccggc
     5161 gtttcgcccg gccgctaccg ccgcgctgcc gccgaagctg cggccgcgcc tggctgagct
     5221 tcgattacag tgcggggccg atctgacgtg cacgtcaccc gatgtcgcta cccgccctgc
     5281 tgcgcgaccg cctgcgcctg cccgtgatcg gcgcgccgct gttcatagtc tccaacccga
     5341 cactcgtgat cgagcagtgc aaggccggca tcgtcggcgc gttcccggcg ctgaacgcgc
     5401 gcccggccca cgtgctcgac gagtggctgg tccgcatcaa gt
//
LOCUS       DBNHBY010000589         3111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_648809, whole genome shotgun sequence.
ACCESSION   DBNHBY010000589 DBNHBY010000000
VERSION     DBNHBY010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_648809"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="AC16HB_14300"
     CDS             complement(<1..1083)
                     /locus_tag="AC16HB_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5608004.1"
                     /translation="MPATHEVFNQSSPFVDFNLYLGNRALRDALRFNHPGFDEQRFIA
                     LGDEVGSGAMQVHARLANTYLPELRNYDRFGHRIDQVEFHPSYHALMSAALRHRLHGA
                     PWAEGAGGHVERAAAFMLFTGLEPSVLCPVSMTYAVTPALRGNAALRAAWGPKVAATQ
                     YDGRFVPFTEKTAVTLGMGMTEKQGGSDVRANTTRAEFEGTDDWGRRYRITGHKWFMS
                     APMCDAFLVLAYAGDALSCFLLPRILPDGRVNALRVERLKHKLGNQANASSEVEFEHA
                     TAWLVGEEGRGVPQILAMGALTRLDCALGTAGLMRQALALALHHCAERSAFGKRLAEQ
                     PMMKNVLADLALESEAATALAVRLARA"
     gene            complement(1144..2949)
                     /gene="recQ"
                     /locus_tag="AC16HB_14305"
     CDS             complement(1144..2949)
                     /gene="recQ"
                     /locus_tag="AC16HB_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIR5608005.1"
                     /translation="MPTRSPSQVLKEVFGYDAFRGFQREVIEHVIAGGDALVLMPTGG
                     GKSLTYQIPALLRDGVAIVISPLIALMQDQVAALDELGVRAAFLNSTLSARDAAAVEA
                     KLRHGELDLVYVAPERLLTERCLNLLGGCRLALFAIDEAHCVSQWGHDFRPEYGQLSL
                     LRERWPQVPRVALTATADVPTRREIVDKLLIDAREFVASFDRPNIRYRIVEKYQPREQ
                     LLRFIRDEHPGDAGIVYCLARNTVEEVAQFLVGHGIPALPYHAGMDAATRAEHQRRFK
                     ERDGQVIVATIAFGMGIDKPDVRFVAHLDMPKSIEGYFQETGRAGRDGLPADAWMTYG
                     LQDVVQQRRLIEMSEADGQYKRLSTAKLDSMLGLAETADCRRVRLLDYFGEASAPCGN
                     CDNCLAPPAQRDATEDARKLLSCIYRCQQASGFGFAATHVIDVLRGKSTEKTARFQHD
                     KLSTFGIGADVPEAEWRLLLRQLVALNVVEVDYARFNVLKLTEASRAVLRGERKIELR
                     RQTERLPKAKRTVANVPVAGADDALFQRLRAWRSDIAKEHGVPAYVVFQDGTLAAIAQ
                     RRPATLQELRGIAGVGDRKLERYGAQLLTLVTDAA"
BASE COUNT          460 a         1101 c         1056 g          494 t
ORIGIN      
        1 cgcacgcgcc agccgcaccg cgagcgcggt cgcggcttcg ctttcgagcg cgaggtcggc
       61 aagcacgttc ttcatcatcg gctgctcggc gagccgcttg ccgaacgcgc tgcgctctgc
      121 gcagtgatgc agcgcgagcg ccagcgcctg ccgcatcagc ccggcggtgc cgagcgcgca
      181 gtcgagtcgc gtcagcgcgc ccatcgccag gatctgcggc acgccgcgcc cctcctcgcc
      241 gaccagccac gcggtggcgt gctcgaactc gacctcggaa ctcgcgttgg cctgatttcc
      301 gagcttgtgc ttcagacgtt cgacccgcag cgcgttgacg cggccgtcgg gcaagatgcg
      361 cggcagcagg aaacacgaca gcgcgtcgcc cgcgtacgcg agcacgagaa acgcgtcgca
      421 catcggtgcc gacatgaacc acttgtgccc ggtgatgcgg taacggcggc cccagtcgtc
      481 ggtcccttcg aactcggcac gcgtggtgtt ggcgcgcacg tcggagccgc cctgcttctc
      541 cgtcatgccc atcccgagcg tgaccgcggt cttctcggtg aacggcacga aacgcccgtc
      601 gtactgcgtg gccgccacct tcggccccca tgccgcgcgc agcgcggcat tgccgcgcag
      661 cgccggggtc acggcgtagg tcatcgacac cgggcacagc accgagggct ccagtcccgt
      721 gaacagcatg aacgccgccg cgcgctcgac gtggccgccc gctccctcgg cccagggtgc
      781 gccgtgcagc cggtggcgca gcgccgcact catcagcgcg tgatagctgg ggtggaactc
      841 gacctggtcg atgcggtggc cgaagcggtc gtagttcctc agctccggca ggtaggtgtt
      901 ggccaggcgc gcatgcacct gcatcgcgcc cgagccgacc tcgtcgccga gcgcaatgaa
      961 gcgctgctcg tcgaagcccg gatggttgaa gcgcagcgcg tcgcgcagcg cccggttgcc
     1021 gagatagaga ttgaaatcga cgaaggggct gctctggttg aagacctcgt gcgtggcggg
     1081 catggccgct ctcctgctgc gcgatgcgca agtgtaggtc gcgcgccgcg ccgcgcggcg
     1141 gaatcaggcc gcgtccgtga ccagggtcag caactgcgcg ccgtagcgct cgagcttcct
     1201 gtcgcccaca ccggcgatgc cgcgcaactc ctgcagcgtc gccggtctgc gctgcgcgat
     1261 cgccgcgagc gtgccgtcct gaaacaccac gtaggcgggc acgccgtgtt ccttcgcgat
     1321 gtcgctgcgc cacgcgcgca ggcgctggaa cagcgcgtcg tcggcgcctg cgacgggcac
     1381 gttcgcgacg gtccgcttcg ccttcggcag gcgttccgtt tgccgccgca gttcgatctt
     1441 tcgctcgccg cgcaagactg cgcggctcgc ctcggtgagc ttgagcacgt tgaagcgggc
     1501 gtagtcgacc tcgaccacgt tcagcgccac cagttgccgc agcagaagcc gccactccgc
     1561 ctcgggcaca tcggcgccga tgccgaaggt ggagagcttg tcgtgctgga acctggccgt
     1621 cttttcggtc gacttgccgc gcagcacgtc gatcacgtgc gtcgccgcga agccgaagcc
     1681 gctcgcctgc tggcagcggt agatgcacga caggagcttt cgcgcgtctt cggtcgcgtc
     1741 gcgctgcgcc ggcggcgcca ggcagttgtc gcagttgccg catggcgcgc tcgcctcgcc
     1801 gaagtagtcg agcaggcgca cgcgccggca gtcggccgtc tcggccagcc cgagcatgct
     1861 gtcgagcttg gcggtcgaca ggcgcttgta ctggccgtcg gcctcggaca tttcgatcag
     1921 ccggcgctgc tgcaccacgt cctgcagccc gtaggtcatc caggcatcgg ccggcaagcc
     1981 gtcgcgaccg gcgcggccgg tctcctggaa gtagccctcg atgctcttcg gcatgtccag
     2041 gtgcgcgacg aagcgcacgt cgggcttgtc gatgcccatg ccgaacgcga tcgtcgccac
     2101 gatcacctgc ccgtcgcgct ccttgaagcg gcgctggtgc tccgcacgcg tcgcggcgtc
     2161 catgccggcg tgatagggca gcgcggggat gccgtggccg acgaggaatt gcgcgacctc
     2221 ctcgaccgtg ttgcgcgcca ggcaatagac gatgcccgcg tcgcccgggt gctcgtcacg
     2281 gatgaaccgc agcagttgct cgcgcggctg atacttctcg acgatccggt agcggatgtt
     2341 gggccggtcg aagctggcga cgaattcgcg cgcgtcgatc aacagcttgt cgacgatctc
     2401 ccgccgggtc ggcacgtcgg ccgtcgcggt caacgcgact ctgggcacct gcggccagcg
     2461 ttcgcgcagg agcgacagtt gcccgtactc ggggcggaag tcgtggcccc attgcgagac
     2521 gcagtgcgcc tcgtcgatcg cgaacagcgc cagccggcag ccgccaagca gattcaggca
     2581 gcgctcggtc agcagccgct cgggtgcgac gtagacgagg tcgagttcgc cgtgccgcag
     2641 cttcgcctcg accgcggcgg catcgcgcgc cgacagcgtc gaattgagga acgccgcgcg
     2701 cacgccgagt tcgtccagcg ccgcgacctg gtcctgcatc agcgcgatca gcggcgagat
     2761 cacgatcgcc acgccgtcgc gcaacagcgc cgggatctgg tacgtcagtg actttccccc
     2821 gccggtgggc atcagcacca gcgcgtcgcc gcccgcgatc acgtgctcga tcacctcgcg
     2881 ctggaagccg cgaaaagcgt cgtagccaaa gacctccttc agcacctgcg acggactgcg
     2941 cgttggcatg gctgaagcgt agcaatggca gatgaaggag gtggcgctcc cgcgtagcat
     3001 cgcgccatcg cgaccgcaga gagtcccatg agcgccacga tcgatgccgt accaccgacg
     3061 cgcgagttca ccgccgcctg gcttgcctat gcgctgttcg cagtcggcgt g
//
LOCUS       DBNHBY010000590         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_107569, whole genome shotgun sequence.
ACCESSION   DBNHBY010000590 DBNHBY010000000
VERSION     DBNHBY010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_107569"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="AC16HB_14310"
     CDS             complement(<1..1040)
                     /locus_tag="AC16HB_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608006.1"
                     /translation="MTTPADRASSVAAAGTPAADRVSLRDFLQLFTSVMLPMFMAAID
                     QTLLAAATPSIAVELGGMHDTTWIALGYLMATMITVPLYGRLGDAYGRRHILLIAIGV
                     FALGSAACGAAQSMQQLSFARVLQGLGGGGLMVMAQALIGELLPPRQRPRFQGYFAAN
                     FTLASILGPVIGGVVVAHASWRWLFLANVPLALLAAWRVRRLPAERSATASSPFDDAA
                     GLLLFALATASTLVWLTFAGHRFAWASGPSAALGLAALALAWLLVRRERTHPAPLLPL
                     ELLRVRGVAAMAGTVVLFASCFFACVFFLPLYLRLGAGTGAVHAGLLLLPVTLGMVLG
                     STLTGRIVARTG"
     gene            complement(1037..>2296)
                     /locus_tag="AC16HB_14315"
     CDS             complement(1037..>2296)
                     /locus_tag="AC16HB_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5608007.1"
                     /translation="QPRGAWWQVFGDADLDALEQKVEVSNQNLRAAAARYSQALALAS
                     QARAAFFPTVAAAVSSTRAQSAVSGRSATATTDSVQLSAAWEADIWGRVRRSVESSEA
                     GAQASRADLEGTRLSLQAQLAQDYFLLRIADAQIRLLDETVTAYARSLELTRNRYAAG
                     VVTRADVATAETQLAGARAQRVDINVSRAQLEHAIAVLTGAAPADFSLPARELLPTAQ
                     PLPQVPALLPSQLLERRPDIAGAERRAAAANAQIGVAQAAYFPTLTLSASGGWRGPSF
                     GDLFSLPNRVWSLGPALAATLFDAGARSAATEAARAGFDEAAAQYRQTVLAALQEVED
                     NLVALRVLAEEADLQREAVRAARDALDLTTNQYKAGTVSYLNVIIAQTTLFSAQRGEL
                     DVRSRQMTANVTLIRALGGGWAGLAAQ"
BASE COUNT          359 a          838 c          812 g          287 t
ORIGIN      
        1 tgtccggtgc gcgcgacgat gcgcccggtc agcgtcgagc cgagcaccat gccgagcgtg
       61 accggaagca gcagcaggcc cgcatgcaca gccccagtcc cggcgccgag ccgcaggtag
      121 agcggcagaa agaacacgca agcgaagaag caggacgcga acagcacgac tgtgcctgcc
      181 atcgccgcca cgccgcggac gcgcaacagt tccagcggaa gcagcggcgc cgggtgcgtg
      241 cgctcgcgcc gcaccagcag ccacgcaagc gcgagggcgg cgagcccgag tgccgcgctc
      301 ggtcccgacg cccaagcgaa gcgatgtcct gcgaaagtca gccacacgag cgtgctggcg
      361 gtcgccagcg cgaacagcaa caagccggcg gcgtcgtcga acgggctgct cgcagtcgcg
      421 gaacgctccg ccggcaatcg gcggacgcgc cacgccgcga gcagcgccag cggaacattg
      481 gcgaggaaga gccagcgcca gcttgcgtgc gcgacgacca cgcccccgat caccggcccc
      541 agaatgctcg ccagcgtgaa attggccgcg aaatacccct ggaagcgtgg ccgctgccgc
      601 ggcggcagca gttcgccgat cagtgcctgc gccatcacca tcagcccgcc gccgccgagt
      661 ccctgcagca cgcgcgcgaa cgacagttgc tgcatcgact gcgccgcgcc gcacgctgcc
      721 gagcccaggg cgaacacgcc gattgcgatc agcaggatgt gcctgcggcc gtaggcatcg
      781 ccgaggcggc cgtacagcgg cacggtgatc atcgtcgcca tcaggtagcc gagcgcgatc
      841 cacgtggtgt cgtgcattcc gcccagctcg accgcgatgg atggcgtcgc ggcggccagc
      901 agcgtctggt cgatggcggc catgaacatc ggcagcatca ccgacgtgaa gagctgcagg
      961 aaatcacgca gcgatacgcg atccgctgcc ggtgttcccg ccgccgcgac gctcgacgcc
     1021 cggtccgccg gcgtggtcac tgcgcggcga gtccggccca gccgccgccg agcgcacgga
     1081 tcagcgtgac gttggcggtc atctgccggc tgcgcacgtc gagttcgccg cgctgcgcgc
     1141 tgaacagcgt cgtctgcgca atgatcacgt tcaggtagct gacggtgccg gccttgtact
     1201 ggttggtcgt caggtcgagc gcatcgcgcg cggcgcgcac ggcctcgcgc tgcaggtccg
     1261 cttcctccgc cagcacgcgc agcgcgacga ggttgtcctc gacttcctgc agcgcggcga
     1321 ggacggtctg ccggtattgc gccgccgcct cgtcgaagcc ggcgcgggcg gcctcggtcg
     1381 cagcgctgcg cgcgccggca tcgaacagcg tggccgccag cgcggggccg agcgaccaca
     1441 cgcgattcgg cagcgagaac agatcgccga agctgggccc gcgccagccg ccgctcgccg
     1501 acaacgtcag cgtcggaaag tacgcggcct gcgcgacgcc gatctgcgca ttcgcggctg
     1561 cggcacgtcg ctccgcgccg gcgatatcgg ggcggcgctc gagcagctgc gacggcagca
     1621 gcgccggtac ctgcggcaat ggctgcgcgg tcggcaacag ctcgcgcgcc ggcagggaaa
     1681 agtccgccgg cgcggcgccg gtcagcaccg cgatcgcgtg ttcgagctgc gcgcggctga
     1741 cgttaatgtc gacccgctgc gcgcgcgcgc ccgcgagctg cgtttcggcc gtggcgacat
     1801 cggcccgtgt cacgacgccg gcggcataac ggttgcgcgt cagctcgagc gagcgcgcgt
     1861 acgccgtcac cgtctcgtcg agcaggcgga tctgcgcgtc ggcgatgcgc agcaggaaat
     1921 agtcctgcgc cagctgtgcc tgcagcgaca ggcgcgtgcc ctccaggtcg gcgcggctcg
     1981 cctgtgcgcc ggcttcgctc gactcgacgc tgcggcggac gcggccccag atatcggcct
     2041 cccaggcggc ggacaactgc acgctgtcgg tcgtcgccgt cgcactgcgc ccgctcacgg
     2101 cgctctgcgc gcgcgtggac gaaacggccg cagcaaccgt cgggaagaac gcggcgcgcg
     2161 cctgcgacgc cagcgccagc gcctggctgt agcgcgcggc ggcggcgcgc aggttctggt
     2221 tcgacacctc gaccttctgt tcgagtgcat cgaggtcggc atcgccgaac acctgccacc
     2281 acgcgccgcg cggctg
//
LOCUS       DBNHBY010000591         3556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_311710, whole genome shotgun sequence.
ACCESSION   DBNHBY010000591 DBNHBY010000000
VERSION     DBNHBY010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_311710"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /gene="ttcA"
                     /locus_tag="AC16HB_14320"
     CDS             <1..800
                     /gene="ttcA"
                     /locus_tag="AC16HB_14320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR5608008.1"
                     /translation="MIEHGDRVMVCLSGGKDSYGLLDVLLKLRERAPIAFEIVAVNLD
                     HRQPGFPAHVLPDYLSALGVPFRIETQDTYSIVTRVIPQGKTYCSLCSRLRRGILYRV
                     AGELGATKIALGHHRDDILGTFFLNLFYGGKLKAMPPKLVSDDGKHIVIRPLAYVKEA
                     DLARYAQVRQFPIIPCNLCGSQPNLKRQEVKRMLAEWEKRFPGRVENSFNALARVVPS
                     HLLDATLFAFAGLSASGMADTQGDIAFDEESPCDPPALAVHIDARDE"
     gene            complement(885..2072)
                     /locus_tag="AC16HB_14325"
     CDS             complement(885..2072)
                     /locus_tag="AC16HB_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608009.1"
                     /translation="MSMIVRIACLAMIFMGTMFIPLRVGAQTPQQMEYERQQREYWRQ
                     QEQQRQEQQRQQQIMNENARRQQEESSRINAPRGQGATPDSSRGPQGGGQYPGGGQAA
                     ELEKARQTWLQRPALAADRNPLLGKWMRPASTRAKSSDPFAQLGALVQGGLCEMFFGG
                     GGVFEFRPDRMVGSDARTKEMELDRVEYRGDAKRVVVIPKTTFKLIVFDFDGPDRINW
                     AGQDCLLVRVGAAPGHAATPPASNVQAALPPAAKGNAEPGNGAVLSLVAGFSADGGAF
                     SPLAGSKFIVLRRSVDAALASHGYHPPPGVPIYRAWMSACQNKAPGCAQGLQGIQASA
                     LGVLTTDQQGSAHTPALAAGTYYLFGSAKRGAESMMWNVRVELRPGENAVRLDQRNAT
                     ALN"
     gene            complement(2082..2606)
                     /locus_tag="AC16HB_14330"
     CDS             complement(2082..2606)
                     /locus_tag="AC16HB_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608010.1"
                     /translation="MLLLTALVGAGAPPTQAATLGDLARALTRRDADTRDHGCALDAI
                     EGVQWRNRQTPGGSFAREGSVTLADFGPATVVARGARHRMFDVQVSVTSTIDAKTLGR
                     TLRSQFPGDARFEFIRDRSSGKRAVPAARVYRVTLKGREPLYVGVLPAPDGKAGRQAT
                     VFVLASEIKPDWTR"
     gene            complement(2778..3323)
                     /locus_tag="AC16HB_14335"
     CDS             complement(2778..3323)
                     /locus_tag="AC16HB_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608011.1"
                     /translation="MKRAISAICCAAATILLFDPHGFGRPAQAQTAAQKQAQAREAQA
                     RALLAKYTAAPGRDDSDGLLKEPAVRAELQRLVGNQVPTLMQNINVRGSIAFDGGSLG
                     IRGNAPHKGTEEEGVVCVNPFGPALVEAAIFSRGKVTVFTAAEKYEYVTLCVKDWITL
                     VNSGYRDRFKQPKNVQVLRAR"
BASE COUNT          528 a         1246 c         1152 g          630 t
ORIGIN      
        1 acatgatcga gcacggcgac cgcgtgatgg tctgcctgtc cggcggcaag gacagctacg
       61 gcctgctcga cgtgctgctc aagctgcgcg agcgcgcgcc gatcgcgttc gagatcgtcg
      121 ccgtcaatct cgaccatagg cagccgggct ttcccgcgca cgtgctgccc gactacctga
      181 gcgcgctcgg cgtgccgttc cgcatcgaga cgcaggacac ctactccatc gtcacgcgcg
      241 tgattccgca gggcaagacg tactgttcgc tctgctcgcg gctgcggcgc gggatcctct
      301 accgcgtggc gggcgaactc ggcgcgacga agatcgcgct ggggcatcac cgcgacgaca
      361 tcctcggcac gttcttcctc aacctgttct acggcggaaa actcaaggcg atgccgccca
      421 agctcgtctc ggatgacggc aagcacatcg tgatccgccc gcttgcgtac gtgaaggaag
      481 ccgatctggc gcgttacgcg caggtcaggc agttcccgat cattccctgc aacctgtgcg
      541 ggtcgcagcc gaacctgaag cggcaggaag tgaagcggat gctcgccgag tgggagaagc
      601 gattcccagg ccgcgtggaa aactcgttca acgcgctggc gcgggtggtg ccgtcgcacc
      661 tgctcgatgc gaccctgttc gcctttgccg ggttgagtgc aagcggcatg gccgacacgc
      721 aaggcgacat cgcgttcgac gaggagtcgc catgcgaccc gccggcgctg gcggtgcaca
      781 tcgacgctcg cgacgagtga ccgcgcgtca cgtatcagcg tgacggcttc ctctcacttg
      841 cgccccttgt ccgattcacg cttacgacgc gccgctcgtt ccggtcagtt caaggcagtc
      901 gcgttgcgct gatcgagcct gacggcgttc tcgcccggcc gcagttcgac gcggacattc
      961 cacatcatcg actcggcgcc gcgcttggcg ctgccgaaca ggtagtaggt tcccgccgcc
     1021 agcgcgggcg tgtgcgcgct gccctgttga tccgtcgtca gtacgccaag cgcgctggct
     1081 tgaatcccct gcagcccttg cgcgcagccg ggcgccttgt tctggcaggc gctcatccag
     1141 gcccgataga tcggcacgcc cggaggtggg tgatagccgt ggctcgcgag cgccgcatcg
     1201 acgctgcgtc gaagcacgat gaacttgctg cccgcgagtg gactgaatgc gccgccgtcc
     1261 gccgagaagc cggcgaccag actcagtacc gcgccgttgc ccggctccgc attgcccttg
     1321 gccgccggtg ggagtgcggc ctgcacgttt gatgccggcg gcgttgctgc atgcccgggc
     1381 gccgcaccga cacgtaccag caggcagtcc tggccggccc agttgatccg gtccgggcca
     1441 tcgaaatcga acacgatcaa cttgaacgtc gtcttcggaa tgaccaccac gcgcttcgcg
     1501 tcgccgcggt attcgacgcg gtcgagctcc atctccttcg tgcgcgcatc cgagccgacc
     1561 atgcggtccg ggcgaaattc aaacacgccg ccaccgccga agaacatctc gcacaggccg
     1621 ccttgcacca gcgcgccgag ttgcgcgaac gggtccgacg atttcgcgcg tgtcgatgcc
     1681 gggcgcatcc acttgcccag cagcgggtta cggtcggccg ccagcgccgg gcgttgcagc
     1741 caggtctgac gcgctttctc gagttccgcc gcttgtccgc cgcccgggta ctggccgccg
     1801 ccctgcggcc cgcggctgga gtcaggcgtc gcgccctgcc ctctcggcgc attgatccgg
     1861 ctcgactcct cctgctgccg gcgcgcgttc tcgttcatga tctgctgctg gcgctgctgc
     1921 tcctggcgct gctgttcctg ctggcgccag tattcgcgct gctgccgctc gtactccatc
     1981 tgctgcgggg tctgtgcccc gactcggagc gggatgaaca tcgttcccat gaagatcatt
     2041 gccaggcagg cgatacgaac gatcatgctc atcggcttct cctaacgcgt ccaatcgggt
     2101 ttgatctcgc ttgcgagcac aaagacggtc gcttgccggc cggccttgcc gtcgggagcg
     2161 ggcaagacgc cgacatagag cggctcccgg cctttcaacg tcacgcggta cacgcgcgcg
     2221 gcgggcaccg cgcgcttgcc gctgctgcgg tctcgaatga actcgaaccg cgcatcgccg
     2281 gggaactgcg acctgagcgt gcgccccagc gttttcgcgt cgatggtcga ggtgaccgac
     2341 acctgcacat cgaacatccg gtgccgcgcg ccccgggcga cgaccgtggc cggaccgaag
     2401 tcggcaagcg tcacggagcc ttccctcgca aaggaacccc ccggcgtctg ccggttgcgc
     2461 cactgcacgc cctcgatggc atcgagtgcg cacccgtggt cgcgggtgtc cgcgtcgcga
     2521 cgcgtgagtg ccctggccag atcgccgagc gttgctgcct gcgtcggcgg cgcgcccgcg
     2581 cccacgagtg ccgtcagcag cagcaccgaa ctacgcatgc ccgcgccttc ttgcagatgg
     2641 atggatcgcg ctggccggct gcgcgagggc gacccacgca agacggcaag gcgcggcagc
     2701 ggccgcatac cacggagcat tcatctgtct ccccacgcac gctttgagtc gatccgcttt
     2761 ggcgcggcgt cgcggcgtca ccgcgcgcgc agcacctgga cgtttttcgg ctgcttgaag
     2821 cgatcgcgat aaccggagtt gaccagcgtg atccagtcct tcacgcacag ggtcacgtac
     2881 tcgtactttt cggccgccgt gaacacggtc acctttccgc gcgagaagat cgccgcctcg
     2941 accagcgcgg ggccgaaggg attgacgcag accacgccct cctcctccgt gcccttgtgc
     3001 ggcgcgttgc cccggatgcc gaggctgcca ccgtcgaacg cgatcgagcc gcgcacgttg
     3061 atgttctgca tcagcgtcgg cacctgattg ccgacgagtc gctgtagttc cgcgcgcacg
     3121 gcgggttcct tcagcagacc gtcgctgtcg tcgcggcccg gcgccgccgt gtacttcgcc
     3181 agcagtgcac gtgcctgcgc ctcgcgcgcc tgggcctgtt tctgcgccgc tgtctgcgcc
     3241 tgcgccgggc gtccgaagcc gtgcggatcg aacagcagga tcgtggctgc cgcgcagcag
     3301 atggcgctga tcgctctttt catcgcagct tctccccagg gggcgtcgca tgacgcgctt
     3361 cgacgctgaa tgcgatcaat ctagaaccga tccggttact gcgccgttac tggccacgcg
     3421 acgcagcgtg gcgggcgata ccgttgccta gcagcgcagc cgccggctcg ccgtggtgcg
     3481 gatcgcgcgg ttgaccgggt tgcggtcgag aaagctgtcg cggaattcgt ccgggacgtt
     3541 gggcagcgcg acatcg
//
LOCUS       DBNHBY010000592         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1867809, whole genome shotgun sequence.
ACCESSION   DBNHBY010000592 DBNHBY010000000
VERSION     DBNHBY010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1867809"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="AC16HB_14340"
     CDS             <1..993
                     /locus_tag="AC16HB_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608012.1"
                     /translation="TRKYGGTGLGLAISKRLAELMGGRMWAESAGAGQGSTFAFTIRV
                     PVAELPPAQRRDYLGQQPVLAGKRLLIVDDNATNRRVLALQAAKWGMVPRDTGAPAEA
                     LQWVGGGTTFDLAIVDMHMPEMDGLALARRLRERAPALPLALFSSLGRREAGDAEGLF
                     AAHLNKPIRQSQLFDTLMSLLAHADTPKAPAAAKPSIDPGMAARHPLRILLAEDTVVN
                     QKLALRLLSQMGYRADLASNGIEAVESVQRQPYDVVLMDVQMPEMDGLEATRQIVARW
                     PAGERPRIVAMTANAMQGDRQMCLDAGMNDYITKPIRVDQLVEALYQVTPRKDR"
     gene            994..1323
                     /locus_tag="AC16HB_14345"
     CDS             994..1323
                     /locus_tag="AC16HB_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR5608013.1"
                     /translation="MDPAIDPATFTDLQATAGADFVDELIDAYLEEAPQFLADLRSAH
                     AQRAADKFRRAAHSLKSNSNTFGALALGRMARELELGGLPPDDATLGALDAEYARVAA
                     ELTELRR"
     gene            1320..>1978
                     /locus_tag="AC16HB_14350"
     CDS             1320..>1978
                     /locus_tag="AC16HB_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608014.1"
                     /translation="MTETGARLLVVEDNKVNRLLLMRGLELQGHRVACAENGRVALEM
                     LKREPFDLMLLDMEMPEMDGFQVMEQLTRDLALRDLPVIVTSSVEGVEKVARCIELGA
                     EDYLHKPVNPVLLKARINASLEKKRLRDQQKELVQRFATPEVAQDLAQSGFAIGGKRV
                     QATVMFCDIRDFTPLAEAQPPEETIDLLNTFYTLMFDAISGQGGVVNLMQGDGLMAIF
                     GA"
BASE COUNT          343 a          647 c          704 g          284 t
ORIGIN      
        1 acccgcaagt acggcggcac ggggctggga ctggcgatca gcaagcggct ggcggaactg
       61 atgggcgggc gcatgtgggc cgagagcgcg ggggcgggcc aggggtcgac cttcgcgttc
      121 acgatccgcg tgccggtggc ggaattgccg ccggcgcagc gccgtgatta ccttggccag
      181 cagcccgtgc ttgccggcaa gcggctgctg atcgtcgacg acaacgcgac caaccggcgc
      241 gtgcttgcgc tgcaagccgc caagtggggc atggtgccgc gtgataccgg cgcacccgcc
      301 gaggcactgc aatgggtcgg cggtggcacg acgttcgatc tggcgatcgt cgacatgcac
      361 atgccggaga tggacgggct ggcgctcgcg cgccgcctgc gcgaacgtgc gcccgcgctg
      421 ccgctggcgc tcttcagttc actcggccgg cgcgaggcgg gggatgccga aggactgttt
      481 gcggcgcacc tgaacaagcc aatccgccag tcgcagctct tcgacacctt gatgagcctg
      541 cttgcgcacg ccgacacgcc gaaggcgccg gcggcggcca agccgtcgat cgatccgggc
      601 atggccgcgc gccatccgtt gcgcatcctg ctcgccgagg acacggtggt gaaccagaag
      661 ctcgcgctgc ggttgttgtc gcagatgggc taccgcgccg atctggcgtc caacggcatc
      721 gaggcggtcg agtcggtgca gcgccagccg tacgacgtgg tgctgatgga cgtgcagatg
      781 cccgagatgg acgggctcga agcgacgcgc cagatcgtcg cacgctggcc ggcaggcgag
      841 cggccgcgca tcgttgcaat gaccgccaac gcgatgcagg gagatcgcca gatgtgcctc
      901 gatgccggca tgaacgacta catcaccaag ccgatccgcg tcgaccagct cgtcgaggcg
      961 ctgtaccagg tgactccacg caaggaccgc tgaatggatc ccgccatcga tccggccact
     1021 ttcaccgacc tgcaggcgac cgcgggcgcg gacttcgtcg acgaactgat cgacgcctac
     1081 ctcgaggaag cgccgcagtt cctcgccgac ctgcgcagcg cgcacgcgca gcgcgcggcc
     1141 gacaagttcc gccgcgccgc gcattcgctg aagtcgaaca gcaacacgtt cggcgcgctc
     1201 gcgctgggaa gaatggcgcg cgaactggaa ctcggcggcc tgccgccgga cgacgccacg
     1261 ctcggagcgc tggatgccga gtacgcgcgc gtcgcggcgg agctgacgga gctgcgcaga
     1321 tgacggaaac cggcgcacgc ctgctggtgg tcgaggacaa caaggtcaac cggctgctgc
     1381 tgatgcgcgg cctcgaactg cagggccatc gcgtcgcgtg cgcggagaac ggccgggtcg
     1441 cgctcgagat gctgaagcgc gagccgttcg acctgatgct gctcgacatg gagatgccgg
     1501 agatggacgg cttccaggtg atggagcagc tcacgcgcga cctcgcgctg cgcgacctgc
     1561 cggtaatcgt gacctcttcg gtcgaaggcg tggagaaggt ggcgcgctgc atcgaactgg
     1621 gcgccgagga ctacctgcac aagccggtga atccggtgtt gctgaaggcg cgcatcaatg
     1681 ccagcctgga gaagaagcgg ctgcgcgacc agcagaagga actggtgcag cgcttcgcca
     1741 cgccggaggt ggcgcaggac ctcgcgcaat ccggcttcgc aatcggcggc aagcgcgtgc
     1801 aggccaccgt gatgttctgc gacatccgcg acttcacgcc gctcgcggaa gcgcagccgc
     1861 cggaagagac gatcgacctg ctgaacacct tctacacgct gatgttcgac gccatcagcg
     1921 gccagggcgg cgtggtcaac ctgatgcagg gcgacggcct gatggcgatc ttcggcgc
//
LOCUS       DBNHBY010000593         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_988182, whole genome shotgun sequence.
ACCESSION   DBNHBY010000593 DBNHBY010000000
VERSION     DBNHBY010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_988182"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..572)
                     /locus_tag="AC16HB_14355"
     CDS             complement(204..572)
                     /locus_tag="AC16HB_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608015.1"
                     /translation="MRAAAPRLVLLDSVTTVGPQHAGCMVVTGSHGGASVVRYARAVE
                     ALLYVFNDAGIGKDDAGVAALAELERAGVAAAAVSHLTARIGEARDSWEHGVVSRANA
                     QALALGVRVGQALRTSLRIA"
     gene            complement(577..1188)
                     /gene="gstA"
                     /locus_tag="AC16HB_14360"
     CDS             complement(577..1188)
                     /gene="gstA"
                     /locus_tag="AC16HB_14360"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HIR5608016.1"
                     /translation="MKLYFSPGASSLAPHIALAEAGLAFELERVDLKTKKYGKDKDYL
                     KINPKGYIPTLQFEDGSVLTEGPAILQWVADQVPEKHLAPPSGTMERYHLVEWLNFIA
                     SELQKQFSPLFNPTTSDDTKQHQRELLSRRFDYLQGALGSQLYVMGETFTVADAFLFT
                     VLGWGKWVELDLAKWPRLADYHARVGARPKVQSAIAAEKPPKK"
     gene            complement(1341..2135)
                     /locus_tag="AC16HB_14365"
     CDS             complement(1341..2135)
                     /locus_tag="AC16HB_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608017.1"
                     /translation="MTTRPDSAAAYRAANAALDERPSDATRAAILAAAARAVDAKPHA
                     AGGAPRRWRIPLAAAATVLISAIAVIVAQRTERELPAPVAAGGAPAPALQKRADAAGA
                     IVPRAAPDSPERPAAAPVAPAPSARVRPPAVARSAQPTEAAPARSEAVTESSLGEVSA
                     DANAERRDAAGGAQPPVAAAVPKPAARTLDAQGAAERRAEPTAESAATAAGVAATETP
                     ERWLARIAELRRAARDDEADAELKKLRERYPQLVIPESALRRAGTR"
     gene            complement(2132..>2344)
                     /locus_tag="AC16HB_14370"
     CDS             complement(2132..>2344)
                     /locus_tag="AC16HB_14370"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR5608018.1"
                     /translation="VRAMSRAQARAFVAAVERLPVAQREALLLHLEGELTLDEIAQLT
                     GVGMETAKSRLRYALAKLREWLEDWR"
BASE COUNT          315 a          846 c          833 g          350 t
ORIGIN      
        1 cgcgatccag gtcgcggcct tgctgtcctt cgagcaggtg aaacagtgcg cgaatagcgc
       61 aaacgcgcgc ggcgcggtgt cgggcagatc gagccgcgcg gcaagcgcgg ccccggcact
      121 gcccggaaac gtgaccttct gcgacggcat gagccctcct gcagccgaat gtgcgcaccg
      181 tagtcggcgc gcatcgacct gccttacgcg attcgcagcg atgtgcgcaa cgcttgcccg
      241 acgcggacac cgagggccag cgcctgcgca ttggcgcgcg agacaacccc gtgctcccag
      301 gaatcgcgcg cctccccgat gcgcgccgtc agatgcgaca ccgccgcggc cgccacaccc
      361 gcacgttcca gttccgcgag cgccgccacg ccggcgtcgt ccttgccgat cccggcgtcg
      421 ttgaagacgt acagcagagc ctcgacggcg cgcgcgtagc ggaccacgct ggcaccgccg
      481 tgcgagccgg tcaccaccat gcaccccgcg tgctgcggac ccaccgtcgt gacggaatcg
      541 agcaggacga gtcgcggcgc cgccgcgcgc atcgggctac ttcttgggcg gcttctccgc
      601 cgcgatcgcg gattggactt tgggccgcgc acccacgcgc gcgtggtagt cggcgagcct
      661 gggccacttg gccagatcga gctcgaccca cttgccccag ccgagcacgg tgaacaggaa
      721 cgcgtcggcg acggtgaacg tctcgcccat tacgtacagc tggctgccga gcgcgccctg
      781 caggtagtca aagcggcggc tcagcagctc gcgctggtgc tgcttcgtgt cgtcgctggt
      841 ggtcgggttg aacaacggcg agaactgctt ctgcagctcg ctggcgatga agttcagcca
      901 ctcgaccagg tggtaccgct ccatcgtccc agagggcggc gccaggtgct tttccggcac
      961 ctgatcggcc acccactgca ggattgccgg cccctcggtc agcacgctgc cgtcctcgaa
     1021 ctgcagcgtc ggaatgtagc cctttggatt gatcttcagg taatccttgt ccttgccgta
     1081 cttcttcgtc ttcaggtcga cccgctccag ctcgaacgcc agtccggcct cggcgagcgc
     1141 gatatgcggc gcgagcgaac tggcgccggg cgagaaatag agcttcatgg gacggtctcc
     1201 tcgatcgatg gatcgaggga ggatagcaac tcccactcgc ttccgaatcg gccgcgttcc
     1261 acgccgcagc gatcgccggg atgcgcgctc gagcagcggc ggcgtcccga ggctgcgtgt
     1321 cggcgcaacg gcggcgaact tcagcgcgtg ccggcgcggc gcagggcgga ctcggggatg
     1381 acgagttgcg gatagcgctc gcgcagtttc ttcagctcgg catccgcttc gtcgtcgcgt
     1441 gccgcgcggc gcaactcggc gatgcgcgcc agccatcgtt ccggcgtctc ggtcgccgcg
     1501 acgccggccg ccgtggccgc cgactccgcc gtcggctcgg cacgtcgttc ggcggcaccc
     1561 tgggcatcga gcgtgcgcgc ggccggcttc ggcacagcgg cggcgaccgg aggttgtgcg
     1621 ccgccggccg catcgcgtcg ttctgcattc gcgtccgcag agacctcgcc gagcgacgac
     1681 tcggtgactg cttcgctgcg agccggcgct gcctcggtcg gctgcgcgct gcgcgcgacg
     1741 gcgggcggcc gtacgcgcgc cgagggcgcc ggagcaaccg gcgcggcggc gggcctctcg
     1801 ggggagtcgg gcgcggcacg cggcacgatc gcgccagccg catcggcgcg cttctgcagc
     1861 gcgggcgccg gggcgccgcc ggcggccacc ggcgcgggca gttcccgctc ggtgcgctgg
     1921 gcgacgatga ccgcgatcgc gctgatcagc accgtggccg cggcggcgag cggaatacgc
     1981 caacgccgcg gcgcgccgcc ggcagcgtgt ggcttcgcat cgaccgcgcg cgcggcggcg
     2041 gcgaggatcg cggcgcgggt tgcgtccgac gggcgctcgt cgagcgcggc gttggcggcg
     2101 cggtaggcgg ccgcggaatc gggacgggtc gtcatcgcca gtcctccagc cactcgcgca
     2161 acttggcgag cgcgtagcgc agccggctct tggccgtctc catgcccacg cccgtcagtt
     2221 gcgcgatttc gtccagcgtc agttctcctt cgaggtgcag cagcagcgcc tcgcgttgcg
     2281 cgaccggcag ccgttcgacc gcggcgacga acgcgcgtgc ctgcgcgcgc gacatcgcgc
     2341 gcac
//
LOCUS       DBNHBY010000594         5827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_175452, whole genome shotgun sequence.
ACCESSION   DBNHBY010000594 DBNHBY010000000
VERSION     DBNHBY010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_175452"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16HB_14375"
     CDS             complement(<1..202)
                     /locus_tag="AC16HB_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR5608019.1"
                     /translation="MKLTCTAFAPGGEIPARHTCEDKDLSPPLAWRDAPAETRSVALI
                     VDDPDAPDPAAPKMTWVHWVLYN"
     gene            397..816
                     /locus_tag="AC16HB_14380"
     CDS             397..816
                     /locus_tag="AC16HB_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5608020.1"
                     /translation="MANIVVRHDPFDVAFRDFFKSFWRPARWEMEPAFDIRVDVKETD
                     KTYTVRAEIPGVKKEDIDIAIDGNVVTLKAEVKREKEEKDERSVVSERYYGAVSRSFT
                     LDTDVDEKGATAKYGDGVLELVLPKKEGTRARHLRVE"
     gene            986..1630
                     /locus_tag="AC16HB_14385"
     CDS             986..1630
                     /locus_tag="AC16HB_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5608021.1"
                     /translation="MRRLIAQGLLALMLAALAFAAHAQLAYAAKDVHLRAGPARDYPV
                     VAIVPAGSELGVVGCLSDYSWCDVIAGMNRGWVYAGNIGYAYQGGYVPLLNYGAEIGI
                     AVIGFVLLDYWNGYYADRPFYRDRDRWVHHRPRPPRPVGPRAPMPVPPPRGAPGSPRP
                     HPPQHGAPGGQHPQPPHAGAPGGHPPRPPQQGAPGGLRPRPPQQPGPGPRPPGH"
     gene            1647..2339
                     /locus_tag="AC16HB_14390"
     CDS             1647..2339
                     /locus_tag="AC16HB_14390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR5608022.1"
                     /translation="MSTLTKVVSGMMGQLRPKPAKGDSKASIQLAPPQRRGGVPLMEA
                     LAQRQSSREFAPDALPLSLLSGLLWAAFGINRKNGKRTAPTAINAQEIDVFVALPTGA
                     YRYDASAHDLRLVAAADLRRVTGYQDFVDEAPLDLVYVADHARMGLVPVGQRSAYAHV
                     AAGAIAQNVYLFCASSGLATVIRAWIDREAIADALGLTHDQQVLLSQTVGYPEGAAAR
                     AKAKAARGSVST"
     gene            2366..2536
                     /locus_tag="AC16HB_14395"
     CDS             2366..2536
                     /locus_tag="AC16HB_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608023.1"
                     /translation="MTIDFSVAERELLKRILDSYLSELRQEIAATKRDTSPLHSEEDL
                     VKQLQLKVSAAT"
     gene            complement(2590..3405)
                     /locus_tag="AC16HB_14400"
     CDS             complement(2590..3405)
                     /locus_tag="AC16HB_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608024.1"
                     /translation="MWVYLLFALVVAGVLYVVWRQNTQKRRVERERAERRARTDELLA
                     RVLLGEQPSRGQPTAGARPSMAKPVPPPIDRMVRPTAAGQRIDVNALLSDEPDTVAAR
                     ARRQLAQPTNFVGDSVDPSTATHTTAGQPPSPLSLRDGQLDVPLDGLVVAWFEARGYL
                     ARRAPATAQPITLLLSHQEDAARSYAFFYERGRLTAQRAAAVLDKARGLGVGKLLVAA
                     EHGADPAVGSARMPDVQVMDWLAIDRAMKRIDARVAAKILSIARANRGMPTAD"
     gene            complement(3514..5235)
                     /gene="polX"
                     /locus_tag="AC16HB_14405"
     CDS             complement(3514..5235)
                     /gene="polX"
                     /locus_tag="AC16HB_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR5608025.1"
                     /translation="MPVANADIAAAFEQIADLLELRDANPFRVRAYRNAARVVGELKL
                     DLAGQVAAGKSLPKLPGIGPDLSAKIQEFAATGHLAALDRLRKEVPAGVADLLQLPGL
                     GPKRVRALYEELHVHTLPQLLRAARDGRIRSLPGFGSKTEEHIVAAINQKLGEVKRFK
                     LAVATQYAHALLGFLREGPGVEQAIAAGSLRRCRDTVGDIDLLVTAPDGAAVCRHFAA
                     YPDVRDVLQLGDTKASVILRSGLQVDLRVVPSESYGAALMYFTGSKAHNIRLRNLALD
                     RGLKLNEYGLFKGSQVLAGATEEEVYQRLKLPWIAPELREDRGEIEVASKGRLPRLIE
                     RVDLRGDLHAHTNWSDGTASLEAMAQAARAHGLQYIGISEHSRRLTVAHGLDPVRLAK
                     QVHEIGRLNPALSGVQLLTGIEVDILDDGALDLPDAALAPLDVVIAAVHSKFNLPRAK
                     QTARILAALDNPHVKILAHPLGRLIDQRDPYDVDMLAVVRKCKARRIALELNAHPERL
                     DLTDLHCRMAKDEGALVAINSDAHSVHEFDNLVHGIGQARRGWLEAGDVLNTRPLAQL
                     REWLARG"
     gene            complement(5245..5709)
                     /locus_tag="AC16HB_14410"
     CDS             complement(5245..5709)
                     /locus_tag="AC16HB_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5608026.1"
                     /translation="MRAKTVTVRDLATMQVVTVQPQTTLAECAQIMRTQHVGSVIVVD
                     DKRRRDNPVGIVTDRDIVIETVAVDLDAKTLTAGDVMATPLATVNDSDDILDALAKMR
                     ECGVRRLPVVTGDGELAGIIAVDNLLEALAEQLDSIVRVIKAEQTKETALRK"
BASE COUNT          912 a         1957 c         1995 g          963 t
ORIGIN      
        1 ggttgtacag cacccaatgc acccaggtca tcttcggtgc ggcaggatcg ggcgcgtccg
       61 ggtcgtcgac gatcagcgcc acgctccggg tttctgccgg cgcatcacgc caagcgagcg
      121 gcggcgacag gtccttgtcc tcgcaggtgt ggcgcgccgg gatttcgccg cccggcgcaa
      181 acgcggtgca agtgagtttc atcgactcgc ctcccgtctg atggcgtgat ttccagcgtc
      241 gtcgcgcggc cggagcacgg caagcctgcc tgacgcgaac gtggtaacgc cgtcgcgttc
      301 gcgaagccgc ggttcgtcaa tgtcgaaaaa cgccaggcgc gcactatttg tctggtggtc
      361 ggaagtccgg ccgctgctga aacaacggag gaacgtatgg caaacatcgt cgtcagacac
      421 gatccgttcg acgtcgcgtt ccgcgatttc ttcaagagct tctggcgtcc tgcccgctgg
      481 gaaatggagc cggcctttga cattcgcgtc gacgtcaagg agacggacaa gacctacacg
      541 gtccgcgccg aaatccccgg cgtgaagaaa gaggacatcg atatcgcaat cgacggcaac
      601 gtcgtcacgc tgaaggccga agtcaagcgc gagaaagagg agaaggacga gaggtcagtc
      661 gtctccgagc gctactacgg cgcggtgtcg cgcagtttca cgctcgacac cgacgtggac
      721 gaaaagggtg cgacggccaa gtacggcgac ggcgtgctcg agctcgtgct gccgaagaaa
      781 gaaggcacgc gcgcgcggca cttgagagtc gaataagcag caacgtcgcg tcgcttcagc
      841 ggcgcggata aaggccgtgc gtccgcgccg cctcgtcgtc gagttcatgg cccgtgagcg
      901 gaccgacggg ttgagttgcg cctatccgca cgctattggc gagtgtccgc gtggtggccc
      961 gtgactcgat ggcgccgaga tgggtatgag acgcctgatc gcgcagggtc tgttggcatt
     1021 gatgctggcg gcgctggcat tcgcagcgca cgcgcagctc gcgtatgcag cgaaggatgt
     1081 gcatctgcgc gccggcccgg cgcgggacta tccggtcgtg gccatcgtgc cggccgggtc
     1141 ggaactcggc gtcgtcggct gcctgagcga ttacagctgg tgcgatgtca tcgccggcat
     1201 gaaccgcggg tgggtctacg ccggcaacat cggctatgcg tatcagggcg gctacgtgcc
     1261 gctgttgaac tacggcgccg agattggcat tgcggtgatc ggatttgtcc tgctcgacta
     1321 ttggaacggc tattacgccg atcgaccgtt ctaccgggac cgcgatcgct gggtgcacca
     1381 ccggccgcgt ccgccccggc ccgtcggtcc aagggcaccg atgcccgtgc caccgccgcg
     1441 cggcgccccc ggaagcccgc gaccgcatcc gccgcagcac ggtgcgccgg gcggtcaaca
     1501 cccgcagccg ccgcatgcag gggcgcccgg tggtcatccg ccgcggcctc cgcagcaggg
     1561 cgctcccggt gggttgcgtc cgcgaccacc tcagcaaccc gggccgggcc cgcgcccgcc
     1621 gggccactga ctcggggaga tcgacggtga gcacgttgac caaggtggtg agcgggatga
     1681 tgggccagct gcggccgaaa cctgccaagg gcgattcgaa agcctcgatc caactggctc
     1741 cgccgcagag gcgcggtggc gtgccgctga tggaggcgct ggcgcagcgc cagtcatccc
     1801 gcgaattcgc cccggacgcg ctgccgttgt cgttgctgtc cgggctgctg tgggcggcct
     1861 tcgggatcaa tcggaagaac ggcaagcgca ccgcgcccac tgccatcaat gcgcaggaga
     1921 tcgatgtgtt cgtcgcgctg ccgaccggcg cttatcgtta cgacgcaagc gcgcatgacc
     1981 tgcgactcgt cgcggccgcc gatctgcggc gcgtgacggg ttatcaggat ttcgtcgacg
     2041 aagcgccgct cgacctcgtg tacgtggctg atcacgcgcg catgggcctg gtgccggtcg
     2101 gacagcgcag cgcatacgca cacgtcgcgg ccggggcgat tgcgcagaat gtgtatctct
     2161 tctgcgcgag cagcggcctg gccacggtga tccgcgcctg gatcgatcgc gaggcaatcg
     2221 ccgacgcgct cgggctgacg cacgaccagc aggtcctgct gtcacagact gtcggctacc
     2281 cggagggcgc cgcggctcgc gcgaaggcga aagccgcgcg cggctctgtt tccacctgat
     2341 gccttgatca cgacggaggc acaacatgac gatcgatttc agcgtggccg aacgagagct
     2401 gctgaaaagg atcctcgatt cctatctttc ggagttgaga caggagattg ccgcaacgaa
     2461 gcgcgacaca tcgccgctgc attcggaaga ggatctggtc aagcaacttc agctgaaggt
     2521 ttccgccgcg acgtgacgct gcgttcctgg aagcgcccct atcggcgcgc ggaagtcggg
     2581 aggcgtcact caatccgcgg tcggcattcc tcgattggca cgggcaatcg acaggatctt
     2641 ggccgcgacc cgagcgtcga tccgcttcat tgcgcggtcg atcgcaagcc agtccatcac
     2701 ctggacgtcg ggcatccggg ccgatccgac ggcggggtcg gcaccgtgtt ccgcggcgac
     2761 cagcagcttg ccgaccccaa gcccgcgcgc cttgtcgagc accgcggccg cgcgctgcgc
     2821 cgtcagccgt ccgcgctcgt agaagaaggc atagctgcgc gccgcgtctt cctgatggct
     2881 cagcagcagc gtgatcggct gtgccgtggc cggcgcgcga cgcgcgaggt aaccccgggc
     2941 ctcgaaccat gccacgacga gaccatcgag cggaacgtcg agctgtccgt cgcgtagcga
     3001 caggggcgaa ggcggctgcc cggcggtcgt gtgcgtcgct gtcgaaggat cgacgctgtc
     3061 accgacgaaa ttcgtcggct gtgcaagctg gcgccgcgcg cgagcggcga ctgtatcggg
     3121 ctcatcgctc aacagggcgt tgacgtcgat gcgctgaccg gcggccgtcg gccggaccat
     3181 gcggtcgatc ggcggcggca ccggcttggc catcgacggc cgtgcgcccg ccgtcggctg
     3241 accgcggctc ggttgctcac ccagcagcac gcgcgccagc aactcgtcgg tacgcgcacg
     3301 gcgctcggcg cgctcgcgct cgacccgccg cttctgcgtg ttctgtcgcc agacgacgta
     3361 cagcacgcca gcgactacga gagcgaatag cagataaacc cacatcgggg ccagctcatg
     3421 caggttcggg acttgattgt cccaagcaaa tccggtccgt tgcgccgggg actacggatt
     3481 tgcgcgatcc gtggcgtgcc atgattttcg ccgtcacccc cgcgcgagcc actcgcgcaa
     3541 ctgcgccagc gggcgcgtgt tcagcacgtc gcctgcctcc agccagccgc ggcgcgcctg
     3601 gccgatgccg tgcaccagat tgtcgaactc gtgcaccgaa tgcgcgtcgg agttgattgc
     3661 caccagcgcg ccttcgtcct tcgccatccg gcagtgcaga tcggtgaggt cgagtcgctc
     3721 cgggtgtgca ttcagttcca gcgcgatgcg gcgtgccttg cacttgcgca cgaccgccag
     3781 catgtcgacg tcgtagggat cgcgctggtc gatcagccgg ccgagcgggt gcgcgaggat
     3841 cttcacatgc gggttgtcca gcgcggccag gatgcgggcg gtctgcttcg cgcgcggcag
     3901 gttgaacttc gagtgcaccg ccgcgatcac cacgtcgagc ggcgccagcg cggcgtcggg
     3961 gaggtcgagc gcaccgtcgt cgaggatgtc cacctcgatg ccagtcagca actgcacgcc
     4021 cgaaagcgcc gggttcaggc gcccgatctc atgcacctgc ttggcgagcc gcaccgggtc
     4081 gagcccgtgc gcgaccgtga gccggcggct gtgctcgctg atcccgatgt actgaagccc
     4141 atgcgcgcgc gcggcctgcg ccatcgcttc gaggctggcg gtgccatcgc tccagttggt
     4201 gtgcgcgtgc aggtcgccgc gcagatcgac gcgttcgatc agtcgcggca atctgccctt
     4261 cgaggccacc tcgatctcgc cgcggtcctc acgcagctcg ggcgcgatcc atggcagctt
     4321 gagccgctgg tacacctctt cctcggtcgc gccggccagc acctggctgc ccttgaacag
     4381 accgtactca ttgagcttca gcccgcggtc gagcgcgagg ttgcgcagcc ggatgttgtg
     4441 cgccttcgat ccggtgaagt acatcagcgc ggcgccgtag ctttccgaag ggaccacgcg
     4501 caggtcgacc tgcagccccg acctcaggat cacggacgcc ttcgtgtcgc cgagctgcag
     4561 cacgtcgcgc acgtccggat acgccgcgaa atgccggcac accgccgcgc cgtccggcgc
     4621 ggtgacgagc agatcgatat cgccgacggt gtcgcggcag cggcgcaggc tgccggcggc
     4681 gatcgcctgc tcgacgccgg gcccctcgcg caggaagccg agcagcgcgt gcgcatactg
     4741 tgtggcgacc gcgagcttga agcgcttgac ttcgccgagc ttctggttga tcgcggcgac
     4801 gatgtgttcc tcggtcttgc tgccaaagcc cggcagcgag cggatacgac cgtcgcgcgc
     4861 ggcgcgcagg agttgcggca gcgtgtgcac gtgcaactct tcgtacagcg cgcgcacgcg
     4921 cttgggccca aggccgggca gctgcagcag gtcggcgacg ccggccggca cttccttgcg
     4981 caggcgatcg agcgccgcga ggtggcccgt ggcggcgaat tcctggatct tcgccgacag
     5041 gtcggggccg atgccgggca gcttcggcag cgacttgccg gccgcgacct gccccgcgag
     5101 atcgagcttc aactcgccca ccacgcgcgc agcgttgcgg tacgcgcgca cgcggaacgg
     5161 gttggcgtcc cgcagttcga gcaggtcggc gatctgctcg aaggccgccg cgatgtccgc
     5221 gttggcgacc ggcatcgcgc agacctactt gcgcagcgcg gtttccttgg tctgctcggc
     5281 cttgatcacg cgcacgatcg agtcgagctg ttccgcgagc gcttcgagca ggttgtcgac
     5341 cgcgatgatg ccggccagct cgccgtcgcc ggtcaccacc ggcagccgac gcacgccgca
     5401 ctcgcgcatc ttggccagcg cgtcgaggat gtcgtcgctg tcgttgacgg tcgccagcgg
     5461 cgtggccatc acgtcgcccg ccgtcagcgt cttggcgtcg aggtcgacgg cgaccgtctc
     5521 gatcacgatg tcgcggtcgg tgacgatgcc gaccggattg tcgcggcgcc tcttgtcgtc
     5581 gacgacgatc acgctgccga cgtgctgcgt gcgcatgatc tgcgcacact cggcgagcgt
     5641 cgtctgcggc tgcacggtga cgacctgcat ggtcgcaagg tcgcgtacgg tgactgtttt
     5701 cgctctcatg cgtcctccta tccaatcagg cgcgcgaggc cgaaggccac gccggcggtg
     5761 acgacgcgtt cgacgcccgc ggccgcgccg ctgatgcccg ccgcaagcgc gaacggcacg
     5821 gtcagac
//
LOCUS       DBNHBY010000595         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_40355, whole genome shotgun sequence.
ACCESSION   DBNHBY010000595 DBNHBY010000000
VERSION     DBNHBY010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_40355"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="AC16HB_14415"
     CDS             complement(<1..781)
                     /locus_tag="AC16HB_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5608027.1"
                     /translation="MKIVCIGGGPAGLYFALLMKKLGAHHDISVVERNRAYDTFGWGV
                     VFSDATMDNMRRWDRDSADAIEQAFNHWDDIELHFKGRVIRSGGHGFVGIGRKKLLNI
                     LQARCEAVGVKLVFEREVDADTDFPDADLIVASDGINSKIRTMYAETFRPDIVTRPNR
                     YIWLGTNKLYDAFTFLFEKTEHGWFQAHIYRFDESTSTFIVECPERVWLAHGLDRADQ
                     AQSIEFCERLFANNLRGEKLMTNARHLRGSAWLSFQRVKCEQ"
     gene            complement(857..1126)
                     /locus_tag="AC16HB_14420"
     CDS             complement(857..1126)
                     /locus_tag="AC16HB_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608028.1"
                     /translation="MEVSPFLTMFFSLLGGAFCALSIDGRQRIFRWIAVVASIAAGPL
                     LLAKVIGIAVAAMAISVFTLFFSTFLTVRWQCMTGLQQSHDLGDE"
BASE COUNT          294 a          479 c          485 g          247 t
ORIGIN      
        1 actgctcgca cttgacgcgc tggaaactga gccacgccga gccgcgcagg tggcgcgcgt
       61 tggtcatcag cttctcgccg cgcaggttgt tcgcgaagag cctctcgcag aactcgatcg
      121 actgcgcctg gtccgccctg tccagcccat gcgcgagcca gaccctctcc gggcattcga
      181 cgatgaacgt cgaggtcgac tcgtcgaacc tgtagatgtg cgcctggaac cagccgtgct
      241 cggtcttctc gaacagaaac gtgaacgcgt cgtagagctt gttcgtgccg agccagatgt
      301 agcggttggg ccgcgtgacg atgtcgggcc ggaaggtctc ggcgtacatc gtgcggatct
      361 tcgagttgat gccgtcgctg gcgacgatca ggtccgcgtc ggggaaatcg gtgtcggcat
      421 cgacctcgcg ctcgaatacc agcttcacac cgaccgcctc gcagcgcgcc tgcaggatat
      481 tgagcagctt cttgcggccg atcccgacga agccgtggcc gccggaacgg atcacgcggc
      541 ccttgaagtg cagctcgatg tcgtcccagt ggttgaacgc ctgctcgatc gcgtcggcgc
      601 tgtcgcgatc ccaccgccgc atgttgtcca ttgtcgcgtc cgagaagacg acgccccagc
      661 cgaatgtgtc gtaggcacgg ttgcgctcga cgacgctgat gtcgtggtgc gcgccgagct
      721 tcttcatcag cagcgcgaag tacagcccgg cggggccgcc gccgatgcag acgattttca
      781 tgcagcctcc tcagcccgag gaggcgaagt tgccggctcg ccggtcgcgt gtcaaccggc
      841 gccggtcaga ggcagatcac tcgtcgccga gatcgtgcga ctgctgcagt ccggtcatgc
      901 actgccagcg cacggtgagg aaggtggaga agaacagcgt gaagaccgag attgccatcg
      961 ccgccaccgc gatgccgatc accttcgcca gaagcagcgg gccggcggcg atgctcgcca
     1021 ccaccgcgat ccagcggaag atgcgctgcc gcccatcgat cgacagcgcg cagaacgcgc
     1081 cgccgagcag ggagaagaac atcgtcagaa acggcgagac ttccactgca acacctgtgt
     1141 ttcgatacga cgcacacagt ctgccgcgcg cgacgcattg acgctatccc gaaagaagga
     1201 agaaagcgcc tcgttcgatc gcggcccgga tgtgatcgca attcgattcg cgcagcaaca
     1261 tgtaagcgcc gacaggcagc ggtcacttac gtcggcacga gtgcgatcgt gcgaagcgac
     1321 ggaaggtcga cgatccggag cagatggtgc atcggaatgg cggcgatcca acgccggcac
     1381 cggcacaagg gtcatcgaag cgaactgcga cccgccgtgc gtcgagttgt atcgaagcgc
     1441 aacaacccat ggttcgagga acccgaccgc gcgccgaggg tcgatatatg aaagcgccga
     1501 tgcgg
//
LOCUS       DBNHBY010000596         1558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1055059, whole genome shotgun sequence.
ACCESSION   DBNHBY010000596 DBNHBY010000000
VERSION     DBNHBY010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1055059"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..913)
                     /locus_tag="AC16HB_14425"
     CDS             complement(167..913)
                     /locus_tag="AC16HB_14425"
                     /inference="COORDINATES: protein motif:HMM:NF018066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HIR5608029.1"
                     /translation="MNLAELHSESMARVSNRETLIVGFDTDPAALPALLPRSLAPDGS
                     NTALVEFAAAPDPAGGSRTEFNLLIPALFGAERVLYRAYAAGDAGESPRGRVRLVVVH
                     DTLTGILELHGRPVAIAAMNYRRADLLDARRRFGALSPADTLRQLRPLRVTALADGGL
                     RSAQARLITQPWSDLRVQCAWTGPASLDLLWPATSPLAALPVQAVHGGLHCVVDFRLQ
                     AKQRPSAAAAAPAVETAPRVPMLAVEGGIQ"
     gene            complement(976..>1558)
                     /locus_tag="AC16HB_14430"
     CDS             complement(976..>1558)
                     /locus_tag="AC16HB_14430"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HIR5608030.1"
                     /translation="VAIEAMMKRAVERFGAVDILVNNAGIQHVAPVDDFPLAKWDAII
                     AINLSAAFHTTRTALPAMKKRGYGRIINIASAHALVASPFKSAYVAAKHGIAGFTKTV
                     ALEVAEQNITVNAICPGYVLTPLVQKQIPDTARARGISEEEVVKNVLLAAQPTKKFVT
                     VEQVASLAAYLATEDAASITGAILPVEGGWTAH"
BASE COUNT          235 a          557 c          530 g          236 t
ORIGIN      
        1 gcggcctcga agttcagctg ggcggcgcgc tgcacgcctt cgagcatcgc cgaagcgagc
       61 ccgcgcgtca gctgcgccag cagttcgtag cgctctgcgg tgcgcggcgc ggcaccgaac
      121 tcgatcaggc gcaactcgac gttcggagcg atctcggcca attcattcac tggatccctc
      181 cttcgacggc cagcatcgga acacgcggcg ccgtctcgac cgcgggcgct gccgccgccg
      241 cactcggccg ctgcttcgcc tgcagcctga agtcgaccac gcaatgcagg ccgccgtgca
      301 ccgcctgcac cggcagcgcc gcgagcggcg aggtcgccgg ccacagcaga tcgagcgacg
      361 ccggtccggt ccacgcgcac tgcacgcgca gatcactcca cggctgcgtg atcaggcgcg
      421 cctgcgccga gcgcagtccg ccgtcggcga gcgccgtgac gcgcaaagga cgcaactggc
      481 gcagcgtgtc cgccggcgac agcgcaccga agcgccggcg cgcgtcgagc agatcggcgc
      541 ggcggtaatt catcgccgcg atcgcgaccg gcctgccgtg cagttcgagg atgccggtca
      601 gtgtgtcgtg cacgaccacc agccgcacgc ggccgcgcgg tgattcgccg gcgtcgccgg
      661 cggcatacgc gcggtacagc acgcgctcgg cgccgaacag cgccggaatc agcaagttga
      721 attccgtgcg gctgccgcca gccggatccg gcgccgccgc aaactcgacg agcgccgtgt
      781 tcgatccgtc gggggcgagc gaccgcggca gcagcgcagg cagcgccgcg gggtcggtgt
      841 cgaagccgac gatcagcgtc tcgcgattgc tgacgcgcgc catcgactcc gaatgcagtt
      901 cggccaggtt catgcggtct cctcgatgcg gtgcggatcg cgccccggcg tccgcgctcg
      961 ttgtcggatc gacgctcagt gcgcggtcca gccgccctcg accggcagga tcgcgccggt
     1021 gatcgatgcg gcatcctcgg tcgccagata cgcggccagc gaagcgacct gctcgaccgt
     1081 gacgaacttc ttcgtcggct gcgcggccag cagcacgttc ttcaccacct cctcctcgct
     1141 gatcccgcgc gcccgagcgg tatccgggat ctgcttctgc accagcggcg tcaacacgta
     1201 gccggggcag atcgcgttca cggtgatgtt ctgctcggcg acctcaagcg cgaccgtctt
     1261 cgtgaagccg gcgatgccat gcttggccgc gacatatgcc gacttgaacg gcgaggcaac
     1321 cagcgcgtgc gcggaagcga tgttgatgat gcgtccgtag ccgcgtttct tcatcgccgg
     1381 cagcgcggtg cgcgtggtgt ggaacgccgc gctcaggttg atcgcgatga tcgcgtccca
     1441 cttggcgagc ggaaagtcgt cgaccggcgc gacgtgctgg atgccggcgt tgttgaccag
     1501 gatgtcgacg gcgccgaaac gctcgaccgc gcgcttcatc atcgcctcga tcgccacg
//
LOCUS       DBNHBY010000597         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1868033, whole genome shotgun sequence.
ACCESSION   DBNHBY010000597 DBNHBY010000000
VERSION     DBNHBY010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1868033"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="AC16HB_14435"
     CDS             complement(<1..1147)
                     /locus_tag="AC16HB_14435"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5608031.1"
                     /translation="MHVITLASLAARGALRGKRVFIRSDLNVPQDDAGNITEDTRIRA
                     SVPAIRMALEAGAAVMVTSHLGRPTEGELKPEDSLAPVAKRLGELLGRAVPLVANWVD
                     GVQVAPGSVVLLENCRVNKGEKKNSDALAQKMASLCDVYVNDAFGTAHRAEATTHGIA
                     KFANVACAGPLLAAEIDALTRALGNPRRPLVAIVAGSKVSTKLTILNNLAAKVDRLIV
                     GGGIANTFLLAEGMPIGKSLAEPDLMPECKKVLTTLADKGGTLPLPIDVVVAKEFKAD
                     APHRVTRADDVQPDEMILDIGPSSSLGLAHVIQEAGTIIWNGPVGVFEFDAFANGTHQ
                     LALEIASATARGAFSLAGGGDTLAAIAKYGITDRVSYISTGGGAFLEF"
     gene            1204..1875
                     /gene="rrtA"
                     /locus_tag="AC16HB_14440"
     CDS             1204..1875
                     /gene="rrtA"
                     /locus_tag="AC16HB_14440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HIR5608032.1"
                     /translation="MFVGHYTNADDIESMKPVPAFARAPVVALLGAAIVLALQGWPGA
                     NVVLEYQVARNAAEPWRLLTAHVVHVNARHALVNAVAWIALARLFASELPVARQLFVL
                     LAGAVGVSLGLALCYPAIAWYRGLSGVLHALYFAGAGMGSLRARSTRTRAMALGSIAL
                     FVAGVIKVASEQPQAGATPFAGWLGAPVVPQAHLVGAVCGTLAALCFSAYDAGRDWLS
                     RRRAV"
BASE COUNT          269 a          638 c          683 g          340 t
ORIGIN      
        1 gaaactcgag gaacgcaccg ccgccggtgg agatatagct gacgcgatcg gtgatgccgt
       61 acttggcgat cgcggcgagg gtgtctccgc cgccggcgag cgagaacgcg ccgcgcgcgg
      121 tggcactggc gatctccagc gcgagctggt gcgtgccgtt ggcgaacgcg tcgaactcga
      181 acaccccgac cggaccgttc cagatgatcg tgccggcttc ctggatcacg tgtgcaagcc
      241 cgagcgaact cgacggaccg atgtcgagga tcatctcgtc gggttgaacg tcgtccgcgc
      301 gtgtgacgcg gtgcggggca tcggccttga actccttcgc gaccacgacg tcgatcggca
      361 gcggcagcgt tccgcccttg tccgcgagcg tggtcagcac cttcttgcac tcgggcatga
      421 ggtcgggctc ggcgagcgac ttgccgatcg gcatgccttc ggcgagcagg aacgtgttgg
      481 caatgccgcc gccgacgatc aggcggtcga ccttggccgc gaggttgttc aggatcgtga
      541 gcttcgtcga caccttggag ccggcgacga tcgccaccag cggtcgcctg ggattgccga
      601 gcgcgcgtgt cagcgcatcg atctcggccg ccaacagcgg gcccgcgcag gcgacgttcg
      661 cgaactttgc gatcccatgc gtggtcgcct cggcgcgatg cgcggtgccg aacgcgtcgt
      721 tcacgtagac gtcgcacaac gacgccatct tctgcgcgag tgcgtcgctg ttcttcttct
      781 cgcccttgtt gacgcggcag ttctccagca gcacgacgct gccaggcgcg acctgcacgc
      841 cgtcgaccca gttcgcaacg agcggcacgg cgcggccgag cagttcgccc aggcgcttgg
      901 ccaccggcgc gagcgaatcc tccggcttga gttcgccttc ggtcggacgc cccaggtgcg
      961 acgtgaccat cacggccgcg ccggcctcga gcgccatccg gatcgcgggt accgacgcgc
     1021 ggatgcgcgt gtcctccgtg atgttgccgg catcgtcctg cggcacgttc aagtcggagc
     1081 gaatgaagac gcgtttgccg cgcagcgcgc ctcgtgctgc cagcgatgcg agcgtgatca
     1141 cgtgcatgga ttttccagtc ggaaggatcg gcaccacgcc ggccaacacg cgatgcgacg
     1201 ttgatgttcg tcgggcatta tacgaacgcc gatgacatcg aatcgatgaa acccgtgccg
     1261 gcgttcgcac gtgccccggt ggtcgcactg ctcggcgctg cgatcgtgct ggcgctgcaa
     1321 gggtggcccg gcgcgaatgt cgtgctcgag tatcaggtcg cgcgcaacgc ggccgagccc
     1381 tggcggctgc tgaccgcgca cgtggtccac gtcaatgcac gtcatgcgct ggtgaacgcc
     1441 gtcgcgtgga tcgcgctggc gcgtttgttc gcgtccgagt tgccggtggc gcgccaactc
     1501 ttcgtcctgt tggcgggtgc cgtcggcgtg agcctcggac ttgcgctgtg ctatccggcg
     1561 atcgcgtggt atcgcgggct gtcgggtgtg ctgcatgcgc tgtacttcgc gggcgcaggg
     1621 atggggtcgc tgcgcgcgcg ctcgacgcga acgcgcgcga tggcgctcgg ctcgatcgcg
     1681 ctgttcgtcg ccggcgtgat caaggtggcg tcggagcagc cacaggctgg cgcgacccca
     1741 ttcgcgggct ggctcggcgc gccggtcgtg ccgcaggcgc acctggtcgg cgcggtgtgc
     1801 ggaacactgg cggcgctgtg cttctcggcg tacgacgccg gccgcgattg gctcagtcgc
     1861 cgtcgcgccg tctgaatccc gcgccggccg ccagcgcgat cagcagcagg agttgcgcaa
     1921 acgggatcgc
//
LOCUS       DBNHBY010000598         1705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_175613, whole genome shotgun sequence.
ACCESSION   DBNHBY010000598 DBNHBY010000000
VERSION     DBNHBY010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_175613"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="AC16HB_14445"
     CDS             complement(<1..919)
                     /locus_tag="AC16HB_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5608033.1"
                     /translation="MAAPRKPDFDVVIVGGGMVGTCLAALLAREDALAGWRIALVDPV
                     APKKPDDRAPDLRVSALSRASERILQAARAWEAIEPHAAPYSEMVVWDAASAPTRPDA
                     LRFSAAATSEPNLGHIVENLRVQWALHESPLQRGVTTIRSGLQALDLDTDAARITLED
                     GRRLSCQLVVGADGGQSVARQLAGIGRSGWNYDQTAVVAHLQTEQPHRDTAWQRFLPS
                     GPLAFLPLRDGRVSLVWSTTPAKAESLLAASAEAFSARVTEASDRVLGRATLASGRAG
                     FPLGLWHAREYVKPRLALVGDAAHTIHPLA"
     gene            complement(919..>1705)
                     /locus_tag="AC16HB_14450"
     CDS             complement(919..>1705)
                     /locus_tag="AC16HB_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5608034.1"
                     /translation="DGAQHTLRARLVVAADGVRSLVRETSGIAADHVGYEQSAITAVA
                     ATQRFHDHVAYERFTEHGPIAILPLTEGRCGLVWTRPPAEVERLLALPDAAFLREFEA
                     AFGLRLGRFLRVGARASYPLQLSRADRHTATRLAVVGNAAQGLHPIAGQGFNLGMRDA
                     MSLAEVIADQRAAGTDDPGTNAVLDEYAGWRETDRRRIVAFTDGLVRLFSLPLAPLRG
                     LRSLGLLVFDVLPPAKSALSRLSVGAAERVPRLARGVPLAGAG"
BASE COUNT          236 a          628 c          638 g          203 t
ORIGIN      
        1 ccgcgagcgg gtggatcgtg tgcgcggcgt cgcccaccag cgcgagccgt ggcttcacgt
       61 attcgcgggc gtgccacagg ccgagcggga aacccgcccg accgctggcc agcgtggcgc
      121 ggccgagcac gcggtcgctc gcttccgtga cgcgcgcgct gaaggcctcg gcgctcgcgg
      181 ccagcagcga ctcggccttg gcaggcgtcg tgctccacac cagcgagacg cggccgtcgc
      241 gcagcggcag gaacgcgagc gggccgctcg gcaggaagcg ctgccacgcc gtgtcgcgat
      301 gcggctgctc ggtctgcagg tgcgccacca ccgccgtctg gtcgtaattc cagcccgagc
      361 gaccgatgcc ggcgagctgg cgcgcgacgg actggccgcc gtcggcaccg acgaccagct
      421 ggcacgacag gcgccgaccg tcctcgagcg tgatgcgcgc ggcgtccgtg tcgagatcga
      481 gcgcctgcag gccgctgcgg atcgtcgtca cgccgcgctg cagcggcgat tcgtgcagcg
      541 cccactgcac gcgcaggttc tcgacgatgt ggccgaggtt gggctccgag gtggccgcgg
      601 cggagaagcg cagagcgtct ggtcgcgtcg gcgcgctcgc ggcgtcccag accaccatct
      661 ccgaatacgg cgcggcgtgc ggctcgatcg cctcccaggc gcgcgccgcc tgcaggatgc
      721 gctccgaggc gcgcgaaagc gccgagaccc gcaggtccgg tgcgcggtcg tccggcttct
      781 ttggcgcgac cggatccacc agcgcgatgc gccaccccgc gagcgcgtcc tcgcgcgcga
      841 gcagcgcggc gaggcaggtg ccgaccatgc cgccaccgac gatgacgacg tcgaaatccg
      901 gcttgcgcgg cgcggccatc agccggctcc ggccagcggc acgccgcgtg cgagccgcgg
      961 cacgcgctcc gcagcaccga cgctgagtcg tgacagcgcc gacttggcgg gcggcagcac
     1021 gtcgaagacc agcaggccca ggctgcgcag tccgcgcaaa ggcgcgagcg gcaacgagaa
     1081 caggcgcacc agcccgtcgg tgaaagcgac gatgcggcgc cggtcggtct cgcgccagcc
     1141 ggcgtattcg tcgagcacgg cgttggtgcc cggatcgtcg gtgcccgccg cacgctggtc
     1201 cgcgatcact tcggccaggc tcatcgcgtc gcgcatgccg aggttgaagc cctgccccgc
     1261 gatcgggtgc aggccctgcg cggcattgcc caccacggcg aggcgcgtcg ccgtgtggcg
     1321 gtccgcgcgt gacagctgca ggggatacga cgcccgcgca cccacgcgca ggaaacgccc
     1381 gagccgcagg ccgaaggcgg cctcgaactc gcgcaggaag gcggcgtccg gcaaggcgag
     1441 cagtcgctcc acctcggcgg gcggccgcgt ccacaccagg ccgcagcggc cctcggtgag
     1501 gggcaggatc gcgatcggcc cgtgctcggt gaagcgctcg taggccacgt ggtcgtggaa
     1561 gcgctgcgtg gcggccaccg ccgtgatggc cgactgctcg tagcccacgt ggtcggcggc
     1621 gatgccgctg gtctcgcgca ccagcgaacg cacgccgtcg gcggcgacga ccagccgtgc
     1681 acgcagcgtg tgctgcgcgc cgtcg
//
LOCUS       DBNHBY010000599        11749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1665862, whole genome shotgun sequence.
ACCESSION   DBNHBY010000599 DBNHBY010000000
VERSION     DBNHBY010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1665862"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..174
                     /locus_tag="AC16HB_14455"
     CDS             7..174
                     /locus_tag="AC16HB_14455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR5608035.1"
                     /translation="MVTQIVPAADYYRAETYHQEYFRHNAAQGYCMFVVAPKVEKFRK
                     TFAAKRRRATA"
     gene            complement(182..1237)
                     /locus_tag="AC16HB_14460"
     CDS             complement(182..1237)
                     /locus_tag="AC16HB_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5608036.1"
                     /translation="MTSRHGAEAGRDFFADDEARPDRNGEICAPPFSEELIRLGHRLP
                     AGVFLGTSSWSFPGWQGIVYGGAYTEAQLARGGLAAYAQHPILRAVSIDRGFYQPLET
                     GEYARYARQVPETFRFVVKAPAQVTDAMVRGAHGAPDAPNPHYLDAALARERFVVPAL
                     SGLASRAGPLVFQLTPLPREMVGSAEALQATVARIGEFIAALPRNVDGVAPVYAVEPR
                     NPELLTPRFVRTLRESGARLCISIHARMPAASRQSTALRAMDATTDEGDDWRMKGPLV
                     VRWNLHAGLRYDDAKQRYAPFDRLLDADILTRGTLVHLIHVAIRSGQPAFVIANNKAE
                     GSAPLSCIELARSVVTR"
     gene            complement(1250..1396)
                     /locus_tag="AC16HB_14465"
     CDS             complement(1250..1396)
                     /locus_tag="AC16HB_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608037.1"
                     /translation="MPQRRRTHLVRLALIESLVAAVGIALLLLLGYAIEPDALSAAAG
                     FARF"
     gene            complement(1487..3394)
                     /locus_tag="AC16HB_14470"
     CDS             complement(1487..3394)
                     /locus_tag="AC16HB_14470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608038.1"
                     /translation="MRRLRCLTAALLAAVFLAMLLPGTARADPLSVVDWAATTGTAKP
                     APDAQWTSFDLPLRWTAAEETSLQSVALRLRFKLSGQPTDAWALLLKLATTGGRVSVN
                     DRVIGEIRMADDATDVQWRRPHLLAIDPSVLVAGENVILIQTTYRGGVHALAGVEVGP
                     LTQLSALYERQFFLSSTLPWIGGTLAALLALIFGVLWLRRRETTLGLLTLAALLWVVR
                     SLFFVIETVPLDARLWMQSLFYGSTGVFAAVMTTALLRLAGREVKRDFWLPLAYGVLG
                     PLLLIATNRTAAPYLDDWWLPGLIIIAGTALFYAVLSRVRHHERLQIVVVAAIAITIG
                     AAIHDYALIKGWLPYSNALALHWAGPLLLVALAAPLVDRFMRVLSDAEVARSDLESRV
                     REREQMLKRNYERLRESERVQATASERQRIMQDMHDGLGSQLLSSLMLVERGALSQHQ
                     VAQILRESIDDMRLAIDALAQGNTDLLASLGNLRFRMEPRFRAAGIELTWDARGLPEE
                     ININPDAVLPILRIVQEALTNALKHSHARAVSVVLTVDPADDGPWLQVRVTDNGRGIA
                     GERVGGRGLLNMRSRAGKIGAQLKLESVPGAGTRVKLTYRLEPYHSGVTRGQTQLGMN
                     TEAIIERFRQI"
     gene            complement(3403..4074)
                     /locus_tag="AC16HB_14475"
     CDS             complement(3403..4074)
                     /locus_tag="AC16HB_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608039.1"
                     /translation="MSTEPISILIVEDEPEFRRRFTQIIENEPSMRLVGVASSKRDAQ
                     SLIEREEFDVMLIDLGLPDGNGTELIRQVSQRRPDTDIMVVTVFGDEQHVVSAIEAGA
                     TGYILKDSTPADVISCIKLLRAGGSPVSPVVARSVLRAIRNRMGGTSTRSPTAPENNP
                     LSARETEILQLLAKGMSFNEIGEILGISPHTVTAHIKKIYRKLAVHSRGEAVYEATQM
                     GLLRS"
     gene            complement(4238..4876)
                     /gene="pdxH"
                     /locus_tag="AC16HB_14480"
     CDS             complement(4238..4876)
                     /gene="pdxH"
                     /locus_tag="AC16HB_14480"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR5608040.1"
                     /translation="MTHSIADLRIEYARAALDESHADADPIRQFAHWWDEAIKSQLPE
                     PNAMTLATADASGRPSARVVLLKGFDDNGFVFFTNYESRKGAELTANPRAALLFFWPE
                     LERQVRVDGNVERVPSDESDAYFRSRPLASRIGAWASPQSRPIPGRAWLMARVAEMGV
                     RHGLNPDRPPHWGGFRVRPTAIEFWQGRPSRLHDRLSYRRDGDGWQRQRLAP"
     gene            complement(4873..5490)
                     /locus_tag="AC16HB_14485"
     CDS             complement(4873..5490)
                     /locus_tag="AC16HB_14485"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014906123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR5608041.1"
                     /translation="MNRSQPPRQTPEQMRATLEAALSGWNGRDPLWVFGYGSLIWNPE
                     LDFDRRAPARVHGYHRRLCLRSVRNRGTSDCPGLVAGLDRGGSCCGVAYRVPAAAVHA
                     QFERLWEREMFMGSYRPRWLACRRLDRPQTVNALAFVVRRDAFNYCGKLSEREMIDIL
                     RRACGIYGSSLEYLRSIVHSLRDAGLRDPHLERLAHRAERAATNP"
     gene            complement(5487..6638)
                     /locus_tag="AC16HB_14490"
     CDS             complement(5487..6638)
                     /locus_tag="AC16HB_14490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608042.1"
                     /translation="MRLPQIIALAVFGHIAFVSTRMAGSLYALANNASTFTVGVLMAL
                     FALVPMLTAVPAGRWLDAVGMRRPLLIGMLMILGGALLPAAFSYQVADIAPLLVGASL
                     IGTGMMLVQLTTQNLVGERADPANRAEAFSWLALGASVSGFTGPVITGFLIEGFGHRI
                     AFAVFVLEMLVALAFLRRYWHRLPSRHATARTDVPPPMFDLLRYRDMRNILLLNGLLS
                     SAWDLQTFMIPVHGTRVGLSAGEIGLVLGSFAVATFVVRLALARLSRSYREWQVLTFT
                     LFTAGVAYLLMPFFGSFWPLAACAFLLGLGLGAAQPNVMSLLHSASPEGRIGEALGLR
                     TTVMNSSHVALPLVFGAAGAVAGAGALFGVMALLLGAAGVATLRLRHKR"
     gene            complement(6635..7540)
                     /locus_tag="AC16HB_14495"
     CDS             complement(6635..7540)
                     /locus_tag="AC16HB_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5608043.1"
                     /translation="MQKNQHLFASPVLAWYFVTVWGSGYLASKVGLQYAPPFTFLTLR
                     FAFGIVLLLPLLAWWHAREPLRFPATAREWRHVTIAGVLMHAANLGGSHYSQYLGMSA
                     GVTALVLAAQPLATAFIAAAFMGERLKPYQWLGVVLGLLGVAFVVWHKIDVRAITWGA
                     AAAVLIALASITAGTLYQRVFCPHVDLRAASIVQFVATLILLAPLAVVVEGFTVRWAW
                     PLFASIAFLVILASIFAVNALHTLMRRGEATRVTSLLYLTPIMAVILEWLMFGVVPTA
                     LTVVGIVVTCAGVALVAWAPKDRAA"
     gene            7691..8269
                     /locus_tag="AC16HB_14500"
     CDS             7691..8269
                     /locus_tag="AC16HB_14500"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5608044.1"
                     /translation="MKTVGQKLEAFRVTGVKPGFNHHEENGVSAFEDITEASFAGKWK
                     IIYFYPKDFTFVCPTEIVGFAKLAKDFEERDAVLLAGSADNEFVKLGWRRDHKDLDRL
                     NHWMFADVKGALIDQLGVRDVSAGVPLRATFIVDPDNVIQHVSVNNLNVGRSPEEVLR
                     ILDGLQTDELCPCNRAVGSATLNPTPAAVTLN"
     gene            8358..8894
                     /locus_tag="AC16HB_14505"
     CDS             8358..8894
                     /locus_tag="AC16HB_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5608045.1"
                     /translation="MEFINEIKSLVPDYAKDVRLNLDGTIARSSLPASEAVGVALAAA
                     YAAKSARIVSIIRAGGALSEVEADAALTAAALMGMNNVWYPFVAMSGDPELKSRPAQL
                     RMQAYATHGGVDKRLFELYALAASIVGKCEHCVQSHYALLRQTGMSVDQLRDVGRIAA
                     VVAAAANAVAIEQESVTA"
     gene            complement(9137..10036)
                     /locus_tag="AC16HB_14510"
     CDS             complement(9137..10036)
                     /locus_tag="AC16HB_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5608046.1"
                     /translation="MRILVPVDGSKFSKAAVDFVASRATLIGKDPSIELLNVQAPVPA
                     RAATVVGKATVKSYHDEEAARALKSAVSALAKAGMPAASGYVIGHPAEEIARQADRDE
                     VDLIVMGSHGHGALMQLVVGSVTNGVLAHTTKPVLLVRDRTAPKADSLRVGIAVDGSK
                     YGRAAVRYVLKHRDLFGAAPTLMLIHVVPDFAGAVMPDMAGLALPAFSDDEIKAMQRK
                     AFETAVAPLRKLFEKEGVKFEEVCLSGAAAEEIAAFAKKRKLDVLVLGSHGHGAFKSA
                     VLGSVVTRVAHISTAPLLLVREA"
     gene            complement(10159..10362)
                     /locus_tag="AC16HB_14515"
     CDS             complement(10159..10362)
                     /locus_tag="AC16HB_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR5608047.1"
                     /translation="MKTNVGGMDRMVRIAAGVVLIALTLMNVIGPWGWIGVLPIATGL
                     FRFCPAYLPFGINTCATKPAESR"
     gene            complement(10454..11152)
                     /locus_tag="AC16HB_14520"
     CDS             complement(10454..11152)
                     /locus_tag="AC16HB_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5608048.1"
                     /translation="MQKTSQTPYERVVAAYPSLAGLPPALRDEVERNLNVMRVAAGTP
                     MFREQDSCAAFPIILSGRVRIARTMENGRELVLYDVEPGESCVLSTSCLLGNARYNAH
                     AQCATDVELATLPRAVFDRLVTEHRPFREEVFHLFGERLVRLVELVESIGFQRLDQRL
                     AAALLGKGQHIATSHEQLAHQLAVSRESISRMLKQFEEQGWVKLGRGSIEIMQPRDLR
                     QVATGQSPAAATQG"
     gene            11317..>11749
                     /locus_tag="AC16HB_14525"
     CDS             11317..>11749
                     /locus_tag="AC16HB_14525"
                     /inference="COORDINATES: protein motif:HMM:NF016291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HIR5608049.1"
                     /translation="MNPTERQAMLTIALLAAFADSSSSDAERAEVKRVSESLGGGEVN
                     ASALYQDVLLKRVSVASAAAQLATPELKQLAYELAVGVCDADKLRNDAETRFLAELGN
                     ALGLTQPQIAQPAATADALATMPLAGAAPVTPAGPVVASQPA"
BASE COUNT         1985 a         4081 c         3951 g         1732 t
ORIGIN      
        1 caggagatcg tcacccagat cgtccccgcg gccgactact atcgtgccga gacgtaccat
       61 caggaatact tccgccacaa cgcggcgcag ggctactgca tgttcgtggt cgcgcccaag
      121 gtggagaagt tccgcaagac gttcgcggcg aagcggcgcc gggcgactgc ctgagacgcg
      181 atcagcgcgt caccacggag cgggccagtt cgatgcaact gagcggcgcg ctgccttcgg
      241 ccttgttgtt ggcgatcacg aacgcgggct ggccgctgcg gattgccacg tggatcaggt
      301 gcaccagcgt gccgcgcgtc aggatgtcgg cgtcgagcag gcggtcgaac ggagcgtagc
      361 gttgcttggc atcgtcgtag cggagaccgg cgtgcagatt ccagcgcacg accagcggtc
      421 ccttcatgcg ccagtcgtcg ccttcgtcgg tcgtggcgtc catcgcgcgc agcgcggtgc
      481 tctggcgcga ggccgcgggc atgcgtgcat ggatgctgat gcacagccgc gcaccgctct
      541 cgcgcagcgt gcgcacgaag cggggcgtca gtagctccgg attgcgcggc tcgaccgcgt
      601 acacgggcgc cacgccgtcc acgttgcgcg gcagcgcggc gatgaactcg ccgatgcgcg
      661 cgaccgtcgc ctgcagcgcc tcggccgagc cgaccatctc gcgcggcagc ggcgtgagct
      721 ggaacaccag tggccccgcc ctggaagcaa ggccggatag cgccgggacg acgaatcgtt
      781 cgcgcgccag cgccgcatcg agatagtgcg gattcggcgc gtccggcgcc ccatgcgcgc
      841 cgcgaaccat tgcatcggtg acctgcgccg gcgccttgac gacgaaacga aacgtctccg
      901 gcacctgccg tgcgtagcgc gcgtattcgc cggtttcgag gggttggtag aagccgcggt
      961 cgatgctgac cgcgcgcagg atcgggtgct gcgcataggc cgccaatccg ccccgggcca
     1021 gttgtgcctc ggtataggcg ccaccgtaga cgatgccctg ccagccgggg aagctccagg
     1081 acgatgttcc caggaacacg ccggccggca ggcgatggcc caggcggatc agttcttccg
     1141 aaaagggcgg cgcgcagatc tcgccgttcc ggtcgggccg cgcttcgtcg tcggcgaaga
     1201 aatcgcggcc ggcttcggcc ccgtggcgcg aggtcatcgc gccagtgtct cagaaccggg
     1261 cgaaacctgc agcagcactc aaggcgtcgg gctcgatcgc atagccgagc agcagcagca
     1321 gcgcgatgcc caccgcggct accagcgact caatcagtgc caatcgaacc agatgcgtgc
     1381 gtcgacgttg cggcatggtg catcctcgcg accctgacag gccccgatcg catctgcatg
     1441 caattgcagt gccagggccg cgcgggcgct gccgccggga cgtcggctag atctgccgga
     1501 accgctcgat gatcgcctcg gtgttcatgc cgagctgcgt ctggccgcgc gtcacgccgc
     1561 tgtggtacgg ctcgaggcga taggtgagct tcacgcgcgt cccggcgccg ggcacgctct
     1621 cgagcttcag ttgcgcgccg atcttgcccg cgcgactgcg catgttcagc aggccgcgcc
     1681 cgccgacgcg ctcgccggcg atgccgcgcc cgttgtcggt cacgcgcacc tgcagccagg
     1741 gaccgtcgtc ggccggatcg accgtcaaga ccaccgacac cgcgcgcgcg tggctgtgct
     1801 tcagcgcgtt ggtcagcgcc tcctgtacga tgcgcagaat cggcagcacc gcgtccggat
     1861 tgatgttgat ctcctccggc aggccgcgcg cgtcccatgt cagttcgatg ccggcggcac
     1921 ggaagcgcgg ctccatgcgg aagcgcaggt tgccgagcga cgccagcagg tcggtgttgc
     1981 cctgcgccag cgcgtcgatc gccagccgca tgtcgtcgat cgattcgcgc aggatctgcg
     2041 ccacctggtg ctgcgacagc gcgccgcgct cgaccagcat cagggacgac agcagctgcg
     2101 aaccgaggcc atcgtgcatg tcctgcatga tgcgctgccg ctcggacgcg gtggcctgca
     2161 cgcgctcgct ctcgcgcagc cgctcgtagt tgcgcttgag catctgctcg cgctcgcgca
     2221 cgcgcgattc caggtcggag cgggcgacct cggcgtcgct cagtacgcgc atgaagcggt
     2281 cgaccagcgg cgcagccagc gccaccagca gcagcgggcc ggcccagtgc agcgccagcg
     2341 cattgctgta cggcagccac cccttgatga gcgcgtagtc gtggatcgcc gcgccgatcg
     2401 tgatcgcgat cgcggccacg accacgatct gcagccgctc gtgatggcgc acgcgcgaca
     2461 gcaccgcgta gaacagcgcc gtgccggcga tgatgatcag tcccggcaac caccagtcgt
     2521 ccagatacgg cgccgcggtg cgattggtgg cgatgagcag cagcggcccg agcacgccgt
     2581 acgcgagcgg cagccagaaa tcgcgtttca cctcgcggcc tgccagccgc agcagcgcgg
     2641 tggtcatcac cgccgcgaac acgccggtcg agccgtagaa cagcgactgc atccacaggc
     2701 gcgcgtcgag cggcaccgtc tcgatcacga agaacaggct gcgcacgacc caaagcagcg
     2761 ccgccagcgt cagcagtccg agcgtcgtct cgcgccgccg cagccacagc acgccgaaga
     2821 tcagggccag cagcgcggcc agcgtgccgc cgatccacgg cagcgtcgac gacaggaaga
     2881 actgccgctc atacagtgcg gacagctgtg tcagcgggcc gacctccacg ccggccagcg
     2941 catgcacgcc gccgcgatac gtggtctgta tcaggatcac gttctcgccg gcgaccagca
     3001 cggatggatc gatcgccagc aggtgtggcc gccgccactg cacgtcggtg gcgtcgtcgg
     3061 ccatgcggat ctcgccgatc acgcgatcgt tgacgctgac gcgcccgccg gtcgtggcca
     3121 gcttcagcag cagcgcccac gcgtcggtcg gctgtccgct cagcttgaag cgcagccgca
     3181 gcgccacgct ctgcagcgat gtctcctcgg ccgcggtcca ccgcagcggc aggtcgaagg
     3241 aggtccactg cgcatccggc gccggcttgg cggtcccggt cgtcgcggcc caatcgacga
     3301 ccgacagcgg gtcggcgcgc gccgtccccg gcaggagcat ggcaagaaaa acggccgcca
     3361 gcagggcggc cgtcaggcaa cggaggcgtc gcaccgcgaa catcaactgc gcagcaggcc
     3421 catctgcgtg gcctcgtaaa ccgcctcgcc gcgcgagtgc accgccagct tgcggtagat
     3481 cttcttgata tgcgcggtca ccgtgtgcgg cgagatgccg aggatctccc cgatctcgtt
     3541 gaagctcatg cccttggcga gcaattgcag gatttcggtc tcgcgcgccg acagcgggtt
     3601 gttctccggc gcggtcggcg agcgggtcga tgtcccgccc atgcggttgc ggatggcgcg
     3661 caacacgctg cgcgccacca cggggctcac gggcgagccg ccggcacgca gcagcttgat
     3721 gcaggaaatc acgtcggccg gcgtggagtc cttcaggatg tagccggtgg cgccggcttc
     3781 gatcgcgctg accacgtgct gctcgtcgcc gaacaccgtg acaaccatga tgtcggtgtc
     3841 ggggcgacgc tggctgacct ggcgaatcag ctcggtgccg ttgccgtccg gcagcccgag
     3901 gtcgatcagc atcacgtcga attcctcgcg ctcgatcagc gactgcgcgt cgcgcttgct
     3961 cgacgccacg ccaaccagcc gcatcgacgg ttcgttctcg atgatctggg tgaaacggcg
     4021 ccggaactcg ggttcgtctt cgacgatcag gatcgaaatc ggttcggtgc tcatggtctc
     4081 gggtccgctg gaggagtgcg caaatgcgcg gtggacgcca gaaacaggcg atgcgtccat
     4141 tctacccacg cagaccgccc gtcgggcagt gaaaattcga ccgcgtcggt ctagcgcgaa
     4201 cgcagtaaat cggcccggat tccacccgcc gcggtgctca cggcgccagc cgctgccgct
     4261 gccagccgtc gccgtcgcgc cgataggaaa gccggtcatg cagccgggac ggccggccct
     4321 gccagaactc gatcgccgtc ggccgcacgc ggaacccccc ccagtgcggc ggccggtcgg
     4381 ggttcagtcc gtgacgcacg cccatctcgg ccacgcgcgc catcagccac gcccggccgg
     4441 gaatcggccg gctttgcggg gaggcccagg cgccgatccg gctcgcgagc ggccggctgc
     4501 ggaagtaggc gtcggattcg tcggacggca cgcgctcgac gttcccgtcg acgcgcacct
     4561 ggcgctccag ttcgggccag aagaagagca gggccgcgcg cggattggcg gtcagttccg
     4621 cgcccttgcg gctctcgtag ttggtgaaga agacgaaacc gttgtcgtcg aaccccttca
     4681 gaagcaccac ccgcgccgac gggcgcccgc tggcgtcggc ggttgccagc gtcatcgcgt
     4741 tgggttcggg caactgcgac ttgatcgcct cgtcccacca gtgcgcgaac tgccggatcg
     4801 ggtcggcgtc ggcgtgcgac tcgtcgagcg cggcgcgcgc gtattcaatg cgaagatcgg
     4861 cgatcgaatg ggtcatgggt tcgttgccgc gcgctcagcg cgatgcgcca gtctttcgag
     4921 gtgcgggtcg cgcaggccgg cgtcgcgcag actgtgcacg atgctccgga gatactcaag
     4981 gctgctgccg tagatcccac aggcacggcg caggatgtcg atcatctcgc gctcggaaag
     5041 cttgccgcag tagttgaacg cgtcgcggcg gaccacgaac gccagcgcgt tgacggtctg
     5101 cggccgatcg agtcggcggc aggcgagcca acgcggccgg tacgagccca tgaacatctc
     5161 gcgctcccac aggcgctcga actgcgcatg cacggccgca gccggcacgc gataggcgac
     5221 gccgcagcag gagccgccgc ggtccaggcc cgcgaccagg cccgggcaat cgctggtgcc
     5281 gcggttgcgc accgaacgca ggcacagcct gcggtgatac ccgtgcacgc gcgccggcgc
     5341 gcggcgatcg aaatccagtt ccgggttcca gatcagcgag ccatagccga acacccacaa
     5401 cgggtcgcgg ccgttccaac cggacagcgc cgcttcgagc gtcgcgcgca tctgctcggg
     5461 cgtctggcgg ggcggctgcg accggttcat cgcttgtgtc gcagtcgcag cgtggcgacg
     5521 ccggcggcgc cgaggagcag tgccatcacg ccgaacaacg cgccggcgcc agccacggcg
     5581 ccggccgcgc cgaacaccag cggcagcgcg acgtggctgc tgttcatcac cgtcgtgcgc
     5641 agcccgagcg cttcgccgat gcggccttcc ggcgacgcgc tgtgcagcag cgacatcacg
     5701 ttcggttgcg ccgcgcccag gccaagcccg agcaggaacg cgcaggcggc cagcggccag
     5761 aagctgccga agaacggcat cagcaggtac gccacgcccg cggtgaacag cgtgaacgtc
     5821 agaacctgcc attcgcggta cgagcgcgac agccgtgcca gcgccagccg gaccacgaac
     5881 gtcgccaccg cgaaactgcc gagcaccaat ccgatctctc ccgcgctcag ccccacgcgc
     5941 gtgccgtgca ccgggatcat gaacgtctgc aggtcccagg ccgacgacag gaggccgttc
     6001 agcagcagta tgttgcgcat gtcgcggtag cgcagcaggt cgaacatcgg cggcggcacg
     6061 tccgttcgcg ccgtcgcgtg acgcgacggc agacgatgcc agtacctacg cagaaaggcc
     6121 agcgcgacca gcatctcgag cacgaacacg gcaaaggcga tccggtgccc gaagccctcg
     6181 atcaggaagc cggtgatcac cgggcccgtg aagcccgaca ccgacgcgcc gagcgcgagc
     6241 cacgagaacg cctcggcacg attggccgga tcggcgcgct cgccgaccag gttttgcgtc
     6301 gtcaactgca ccagcatcat gccggtgccg atcagcgatg cgccgaccag cagcggcgcg
     6361 atgtccgcca cctggtacga gaacgcggcc ggcagcagcg ctccgccgag aatcatcaac
     6421 attccgatca gaagcggacg gcgcatgccg accgcatcga gccagcgccc ggccggaacc
     6481 gcggtcagca tcggcaccag tgcgaacagc gccatcagca cgccgacggt gaatgtcgat
     6541 gcgttgttgg ccagcgcgta cagcgatccg gccatgcgcg tggacacgaa ggcgatgtgg
     6601 ccgaacaccg ccagcgcgat gatctgcggc agtctcatgc cgcgcgatcc ttcggcgccc
     6661 acgccaccag cgccacgccg gcgcaggtga cgacgattcc gaccaccgtg agcgcggttg
     6721 gcaccacgcc gaacatcagc cactcgagga tcacggccat gatcggcgtc aggtacagca
     6781 ggctcgtcac gcgggtggcc tcgccgcgcc gcatcagcgt gtgcagtgcg ttgaccgcga
     6841 agatcgacgc caggatcacg aggaacgcta tcgatgcgaa aagcggccac gcccagcgca
     6901 cggtgaagcc ctccaccacg accgccagcg gcgccagcag gatcagtgtc gcgacgaact
     6961 gcacgatcga tgccgcgcgc aggtcgacat gcgggcagaa gacgcgctgg tacagcgtgc
     7021 ccgcagtaat cgatgccagc gcgatgagca ccgccgcggc cgcgccccag gtgatcgcgc
     7081 gcacgtcgat cttgtgccac acgacgaatg cgacgccgag caggccgagc accacgccga
     7141 gccactgata aggcttgagc cgttcgccca tgaacgccgc cgcgatgaat gcggtggcaa
     7201 gcggctgagc tgccagcacc agtgccgtca cgccggccga catgccgagg tattgcgaat
     7261 agtggctgcc gccgaggttg gccgcgtgca tcaggacgcc ggcgatggtg acgtgccgcc
     7321 actcgcgcgc cgtggctgga aaccgcagcg gctcgcgcgc atgccaccat gccagcagcg
     7381 gcagcagcag cacgatgccg aatgcgaatc gcagcgtcag gaacgtgaac ggcggcgcgt
     7441 actggaggcc gaccttgctg gcgagatagc cggaacccca gaccgtgacg aagtaccacg
     7501 ccagaaccgg cgacgcgaac aggtgctgat tcttctgcat ttggcggggc gcattctcgc
     7561 aaacccgcag ccgggcaatc ccgtgcctat cgccgggttt ggaacattca attggaaagt
     7621 gcgcggcgcc gccgccatag ttcgttgcgt cagccgatct gctatccaac ccgatccgac
     7681 aggagcaacg atgaagaccg taggccagaa actcgaagca ttccgggtca ccggcgtgaa
     7741 gccgggcttc aaccaccatg aggagaacgg cgtgtccgcg ttcgaggaca tcaccgaagc
     7801 gtcgttcgcc ggcaagtgga agatcatcta cttctacccg aaggacttca cgttcgtgtg
     7861 tccgaccgag atcgtcggct tcgcgaagct ggcgaaggac ttcgaggagc gcgatgccgt
     7921 gctgctcgcc ggcagcgccg acaacgaatt cgtcaagctc ggctggcgcc gcgaccacaa
     7981 ggacctggac cgcctgaacc actggatgtt cgccgacgtg aaaggcgcgt tgatcgacca
     8041 gctcggcgtg cgcgatgtca gcgccggcgt gccgctgcgc gcgaccttca tcgtcgaccc
     8101 ggacaacgtg atccagcacg tgtcggtgaa caacctgaac gtcggccgca gcccggagga
     8161 agtgctgcgc atcctcgacg gcctgcagac cgacgagctg tgtccgtgca accgcgcagt
     8221 gggctcggcg accttgaacc cgacgccggc tgcggtcacg ctgaactgag aaagcgaggg
     8281 gaagggggcc gggccccctt ccgaccctcc aaacgcatgc gtgcgccgcg ttccgcgacg
     8341 cacggaggaa ggcaacgatg gaattcatca acgagatcaa gtcgctggtc cccgattacg
     8401 caaaggacgt gcgtctcaat ctggacggca cgattgcgcg ctcgtcattg ccggccagcg
     8461 aagcggtcgg cgtggcgctc gcggcggcat atgccgcgaa gagcgcgcgc atcgtctcga
     8521 tcatccgcgc cggcggcgcg ttgagcgagg tcgaagccga cgccgcgctg accgccgccg
     8581 cgctgatggg catgaacaac gtctggtatc cgttcgtcgc aatgagcggc gacccggagc
     8641 tgaagtcgcg cccggcgcag ctgcgcatgc aggcgtacgc gacccacggc ggcgtcgaca
     8701 aacgcctgtt cgagctctac gcgcttgctg ccagcatcgt cggcaagtgc gagcactgcg
     8761 tgcaatcgca ttacgcgctg ctcaggcaga ccggcatgag cgtcgatcag ttgcgcgatg
     8821 tcggccgcat tgccgcagtg gtcgcggcgg cggcgaacgc ggtggcgatc gagcaggagt
     8881 cggtgactgc ttgactgcgg ctgcccgctg ctcgaaaccc gagcgagcaa cgggcaacga
     8941 cgcacggctc cgactcgatt cccgtcactt caacagatcg atcgtcctgg cgatgatccg
     9001 ggcgcggaac ggcccgggcg tgtttctatg cggccgccgc ccggcctggc ctgatcgtcc
     9061 gcgggcttca cgttcaactc ggcccgcgtg gacacgttcg ccagttcgtc gagccgcttc
     9121 gccgcggcga tgatgctcag gcctcccgca ccagcagcag cggcgcggtg ctgatgtgtg
     9181 ccacgcgcgt gacgaccgag ccgagcaccg cgctcttgaa cgcgccgtgt ccgtgcgagc
     9241 ccaacaccag cacgtcgagc ttgcgcttct tcgcgaaggc ggcaatctct tccgccgccg
     9301 cgccggacag gcagacttcc tcgaacttca cgccttcctt ctcgaacagc ttgcgcagtg
     9361 gtgcgacggc ggtctcgaac gccttgcgct gcatcgcctt gatctcgtcg tccgaaaacg
     9421 ccggcagtgc gaggccagcc atgtcgggca tcaccgcacc ggcaaagtcc ggcaccacgt
     9481 ggatcagcat gagagtcggt gcggcgccga agaggtcgcg gtgcttcagc acgtacctga
     9541 ccgcggcacg cccgtacttg ctgccgtcga cagcgatgcc gacgcgcagc gagtcggcct
     9601 tcggcgcggt ccggtcgcgc acgagcagca ccggtttggt cgtgtgcgcg agcacgccgt
     9661 tggtcacgga gcccaccacc agctgcatca gcgcgccgtg gccgtgcgag cccatcacga
     9721 tcaggtcgac ttcgtcgcgg tcggcctgcc gcgcgatctc ttcggccggg tgcccgatca
     9781 cgtagccgct cgcggcgggc atgccggcct tggcgagggc ggacacggcg gatttcagcg
     9841 cgcgcgccgc ctcttcgtcg tgatatgact tgaccgtcgc cttgccgacc acggtcgccg
     9901 cgcgcgccgg caccggcgcc tgaacgttga gcagttcgat cgacgggtcc ttgccgatca
     9961 gcgtcgcgcg cgaggcgacg aaatcgaccg cagccttgct gaatttgctg ccgtccaccg
    10021 gaaccaggat tctcatcgga cgcccccgcg ccagtgcttc ctttcgacga atccagtgta
    10081 aggcagccgc gtttgtgcga tcgcgcgcgc ggtcaacttc ggtgcgggcg ccgagtgtgc
    10141 cgacagcgat ggatcgaact accggctctc ggccggcttc gtcgcgcagg tgttgatccc
    10201 gaacggcagg tacgccgggc agaagcggaa cagtccggtg gcgatgggaa gaacgccgat
    10261 ccagccccac gggccgatca cgttcatcag cgtcaatgcg atcagcacga ctcctgccgc
    10321 gatccgcacc atccgatcca ttccacccac gttggttttc atccggaatc ccccttggct
    10381 cgtccgcccc tgcctgcggg cgtgagcgcg gaatctactc ctgcacgtgc gtacggtgat
    10441 gtcagcgacg ctgtcaaccc tgcgtcgccg ccgcgggcga ctggccggtc gcgacctggc
    10501 gcaggtcgcg cggctgcatg atctcgatcg agccgcggcc gagcttgacc cagccttgct
    10561 cctcgaactg cttgagcatg cggctgatcg actcgcgcga cacggcgagt tgatgcgcca
    10621 gctgctcgtg cgaggtggcg atgtgctggc ccttgccgag cagcgcggcc gccagccgct
    10681 ggtcgagacg ctgaaagccg atcgattcga ccagttccac cagccgcacc aagcgctcgc
    10741 cgaagaggtg gaacacttcc tcgcggaacg ggcggtgctc ggtgaccagc cggtcgaaca
    10801 ccgcacgtgg cagcgtggcg agctccacgt cggtggcgca ctgcgcgtgc gcgttgtagc
    10861 gcgcattgcc gagcaggcag ctcgtcgaca gcacgcagct ttcgcccggc tcgacgtcgt
    10921 acagcacgag ttcgcggccg ttctccatcg tgcgcgcgat gcgcacgcgc cccgacagga
    10981 tgatcgggaa cgccgcgcac gagtcctgct cgcggaacat cggcgtgccg gccgccacgc
    11041 gcatcacgtt caggttgcgc tcgacctcgt cgcgcaacgc cggcggcagg ccggccagcg
    11101 acggatacgc ggcgacgacg cgttcgtacg gcgtttgact cgtcttctgc atcgggtcgg
    11161 caggcgcggc aacgtgatcc ggcatgatta tcgcgcgccc gctgcgtcgc tgcggacgtc
    11221 ggcagcggcg cacgcggaca gggaatttcc cgggattgag ttccgccgcg cccgtcgcaa
    11281 catcccggac atcctgaacc gcgcgcgagg aatgcgatga accccaccga aaggcaggcc
    11341 atgctgacga ttgcgctgct ggccgcattt gccgacagca gcagctccga cgccgagcgt
    11401 gcggaagtca agcgcgtgag cgaatcgctc ggcggcggcg aggtcaacgc atcggcgctg
    11461 taccaggacg tgctgctgaa acgcgtttcg gtcgcgagcg cggcggcgca gctcgccaca
    11521 cccgaactga agcagctcgc ctacgaactc gcggtcggcg tgtgcgacgc cgacaagctg
    11581 cgcaacgacg ccgaaacgcg cttcctggcc gagctcggga acgcgctcgg gctcacgcag
    11641 ccgcaaattg cgcagcctgc ggcaaccgcc gatgcgctgg caacgatgcc gctcgccggc
    11701 gcggcgcccg tcacgcctgc cggcccggtc gtggcaagtc agcccgcgg
//
LOCUS       DBNHBY010000600         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_379508, whole genome shotgun sequence.
ACCESSION   DBNHBY010000600 DBNHBY010000000
VERSION     DBNHBY010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_379508"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="AC16HB_14530"
     CDS             <1..1343
                     /locus_tag="AC16HB_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5608050.1"
                     /translation="LDPTGALIMFAGIYYGAMYGGSTTTILLNTPGESASIATALEGN
                     KMAKRGRAGPALATSAIGSFVAGTIATALLTVLAPIVVEIALKFGPAEYFALMVFAFV
                     TVAAVLGASMVRGLTMLFLGLLLGLVGIDDQTGQMRFAFGVPELMDGIDVVVLAVGLF
                     AVGEALYVAAYQSRQHEELEKMSGSLWMSRDDWKRSWPAWLRATAIGFPFGTIPAGGA
                     EIPTFLSYALEKKLTKHPEEFGKGAIEGVAGPEAANNASSAGVLVPLLTLGIPTSATA
                     AILLAAFQNYGIQPGPLLFQTQAALVWGLIASMYIGNILLLILNLPLIGLWVKVLMIP
                     RPLLYAGILVFATLGAYSMHQSVVDLATLYVFGLLGFMMRRWGFPVAPAVIGLILGPL
                     AESQFRRALAISQGDPSVFVSHPISAALLVLTALLVLVPWIYKVVKERRERRAT"
     gene            1494..1775
                     /locus_tag="AC16HB_14535"
     CDS             1494..1775
                     /locus_tag="AC16HB_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5608051.1"
                     /translation="MKSILFVLLIAVAIPAARAALPGDATEGKRLHDANCRGCHDPSV
                     YTRLTRSVRSLDALKQQLESCGHMGQKDVSPAEKQHILKYLNDQFYRFE"
     gene            1894..>2587
                     /locus_tag="AC16HB_14540"
     CDS             1894..>2587
                     /locus_tag="AC16HB_14540"
                     /inference="COORDINATES: protein motif:HMM:NF018352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HIR5608052.1"
                     /translation="MSTRTSLVRAALAAVSMLAAASAAQAETAPLWELGLGAAGLRIP
                     HYRGAAQSGNFVLPFPYLVYRGPILKADREGARATLFERGGVELNLSAQGSPPVDSGD
                     NVARRGMPRLATVGEVGPSLNLRLFENPTRGGRVTLRLPVRAAFAIDSHPRKLGWLAT
                     PNLLWDRRELMTGWNVGLQTGPMWADRALNRYLYGVDDAYAATGRPAYRARGGYSGWT
                     SMFTVSRRVDRWW"
BASE COUNT          393 a          843 c          897 g          454 t
ORIGIN      
        1 agctcgatcc gaccggcgcg ctgatcatgt tcgccggcat ctactacggc gccatgtacg
       61 gtggctcgac caccacgatc ctgctgaaca ctccgggcga atcggcgtcg atcgccaccg
      121 cgctcgaagg caacaagatg gccaagcgcg gccgcgccgg gccggcactg gccacgagcg
      181 cgatcgggtc gttcgttgcc ggcacgatcg ctaccgcgct gctgacggtg ctggcgccga
      241 tcgtggtcga gatcgcgctg aagttcggcc cggccgagta cttcgcgctg atggtgttcg
      301 cgttcgtcac cgtcgcggcg gtgctcggcg cgtcgatggt gcgcgggctg acgatgctgt
      361 tcctcggcct gctgctggga ctggtcggca tcgacgacca gaccggtcag atgcgctttg
      421 cgttcggcgt gcccgaactg atggacggca tcgacgtcgt cgtgctcgcg gtcggactgt
      481 tcgcggtcgg ggaggcgctg tacgtcgccg cctaccagag ccggcagcac gaggaactgg
      541 agaagatgtc cggatcgctg tggatgtcgc gcgacgattg gaaacgctcg tggcccgcat
      601 ggctgcgcgc gaccgcgatc ggctttccgt tcggcacgat cccggccggc ggcgcggaga
      661 ttccgacctt tctgtcgtac gcgctggaga agaagctcac caagcatccg gaagaattcg
      721 gcaagggcgc gatcgaaggc gtggccggcc ccgaggccgc caacaatgca tcgtccgccg
      781 gcgtgctggt gccgctgctc acgctcggaa ttcccacgtc ggccacggcg gcgatcctgc
      841 tcgcggcgtt ccagaactac ggcatccagc ccggcccgct gctgttccag acgcaggcgg
      901 cgctggtgtg gggactgatc gccagcatgt acatcggcaa catcctgctc ttgatcctga
      961 acctgccgct gatcgggctg tgggtcaagg tgctgatgat cccgcgccca ctgctgtacg
     1021 cggggatcct cgtgttcgcc acgctcggcg cgtacagcat gcaccagtcg gtggtcgatc
     1081 tggccacgct gtacgtgttc ggcctgctcg gcttcatgat gcggcgctgg ggcttcccgg
     1141 tcgcgccggc ggtgatcggg ctgatcctgg gccccttggc cgaatcgcag ttccgccgcg
     1201 cgctggcgat cagccagggc gacccgagcg tgttcgtgtc gcacccgatc tcggcggcgc
     1261 tgcttgtgct gactgcattg ctcgttctcg tgccgtggat ctacaaggtc gtaaaggaac
     1321 gacgcgaacg acgcgcgacc tgacaccgtg cagttttgcg agtggtctgg gttcaccgcg
     1381 ccactgcgga gtcggtgcgg aagcagcgcg cggcgttgcc cgcgcacccg acaagaagtc
     1441 ggtatagacc ggccagcgat gcgacgagaa tgatctgatc gccggaggca accatgaaat
     1501 cgatcctgtt cgtactgctg atcgccgtcg cgatacccgc cgcacgggcg gccttgccgg
     1561 gcgacgctac cgaaggaaag cggctgcacg acgccaattg caggggctgt cacgatccca
     1621 gcgtctacac gcggctgacg cgctcggtcc ggtcgctcga tgcgctgaag cagcagctgg
     1681 aaagctgtgg ccacatgggc cagaaggatg tgtcgccggc cgagaaacag cacatcctca
     1741 agtacctgaa cgatcagttc taccggttcg agtagcgtcg agatcgggcg ggcgcgggcc
     1801 gggcaggcga accggcgccg cgagcgccgc agttgcgcgt cgagcggcgc gcgcgcacgg
     1861 caggatcttc gttgcgtcct ctacactgcg tcggtgtcga caagaacctc gctcgtccgc
     1921 gcagcgctcg cggcggtgtc gatgctcgcg gccgcctctg cagcgcaagc ggaaaccgcg
     1981 ccgttgtggg aactggggct cggggcggcc gggctccgca tcccgcacta tcgcggcgcc
     2041 gctcaatccg gcaactttgt ccttccgttc ccgtacctgg tctatcgcgg cccgatcctg
     2101 aaggccgatc gcgagggcgc ccgcgcgacg ctgttcgagc gtgggggcgt ggagttgaac
     2161 ctcagtgccc agggcagccc ccccgtcgac agcggcgaca acgtggctcg ccgaggaatg
     2221 ccccgtctgg cgacggtcgg tgaagttggc cccagtctga atctgcgtct gttcgagaac
     2281 ccgacgcgcg gtgggcgtgt gacgctgcga cttcccgtgc gtgccgcatt cgcgatagac
     2341 agccatccgc gcaagctcgg ctggttggcc acgccgaacc tgctttggga ccgccgcgaa
     2401 ttgatgacgg gctggaatgt cggcctgcaa acggggccga tgtgggcgga ccgggcactc
     2461 aatcggtacc tttacggcgt cgacgacgcg tacgcggcga cgggccgccc ggcctatcgc
     2521 gcgcgcgggg gatattccgg ctggaccagc atgttcacgg tgtcgcgtcg cgtcgatcgc
     2581 tggtgga
//
LOCUS       DBNHBY010000601         4942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_920614, whole genome shotgun sequence.
ACCESSION   DBNHBY010000601 DBNHBY010000000
VERSION     DBNHBY010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_920614"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="AC16HB_14545"
     CDS             <1..1371
                     /locus_tag="AC16HB_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5608053.1"
                     /translation="LEAQRDESGKDAAVRLVFEPRTSKIDRDEFVNVLLAHTSLETSV
                     PINLVMIGRDGKPRCKTLREILAEWVEFRVETVRQRTQHRLAKVEDRIHILEGRQAVL
                     LNIDKVIKLIRNSDEPKPDLIKAFKLTERQADDILDLRLRQLARLEGIKIEQELGELR
                     KDQAGLQKLLGSEAALRKQVGREFDEDAKKYGDARRTLIEAAERASVETKVVDEPVTV
                     IISEQGFVRARTGHGHDKTQFTFKPGDALYDAVECRTVDQLIAIGSNGRVYTVPIAQL
                     PSARGDGAPVTSLIELEAGTRVVAFCAGAVTQPLFMSTSSGHGFACQLGDMVTRQKGG
                     KQFITVDEGAVPLRPAGVDPATDDRIACLSANGRLLVFAANEVKMQSGGGRGVTLMGL
                     DSGEPLLAALSCSTAGVIVSGTGKSGKPVEVAVAGADLAAHSGHRARKGTLVKSKLKV
                     SGLRRA"
     gene            1376..2458
                     /locus_tag="AC16HB_14550"
     CDS             1376..2458
                     /locus_tag="AC16HB_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5608054.1"
                     /translation="MKRRSLVAVVAAVAAAGLFVAVAGVPGCVTVNPYYAQSKAHHRP
                     DGFQNIDPAAAMPRPFSEFLRWQAERMTLTISEPKLDLSPLVPDLGYIGKNRTELAVT
                     WIGHATTLLQIGGINVLTDPIFSERASPVQWMGPKRRQPPGVLLRELPHIDVVLISHN
                     HYDHLDEASVKALNAQAAGPPLFVVPLGVERWFADAGITNTKALDWWDAIDVRGTRVH
                     LVPVQHWSGRAFADRMKTLWGGYVIEGGGKRAFFTGDTGYSSAHFREIGERLGPIDLA
                     LIPIGGYEPRWFMAKQHVNPEEAVQIAVDLKARRALGIHWGTFQLTDEPLDQAVADLA
                     AALKKHGLTERQFFVLRHGETRRVLD"
     gene            2475..2939
                     /locus_tag="AC16HB_14555"
     CDS             2475..2939
                     /locus_tag="AC16HB_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR5608055.1"
                     /translation="MKTYALMLFLHLVAIVIWIGGMFLMQFAVRPSAVQLLEPPQRLP
                     LLAEILRRFFNWVAAAAVVVLLSGISIILVAGGFKVVHPSVHLMFGIGVVMIAIFLHI
                     RLAPFPRLRAAVAARDWPAGAMSLDQIRKEVAFNLVLGVAIIAIATLGRVLL"
     gene            complement(2948..3853)
                     /locus_tag="AC16HB_14560"
     CDS             complement(2948..3853)
                     /locus_tag="AC16HB_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5608056.1"
                     /translation="MTRSASYVTVDVFTHQRFGGNPLAVFTDARDLSDREMQSLAAEM
                     NYSETTFVLPPSDPTNTARVRIFNRTSEMPFAGHPNVGTGCVLAGMGRASGDVLRFEE
                     IAGLVEVRIARDAGGAIAGAHIAAPQPLSTGPDLQVDAVAACAGLSAGDLVVRTHPPR
                     IASVGLPFCFAEVTAEALTRAVPDVGAFRRLAQSFHEPPDRLSLHLYARAEGATPNRL
                     RARMFAPLSGTVEDPATGSANAALAAFLLSLGTGEQAQFEIAQGVEMGRPSRLLASAR
                     RGSDGIRATVGGGAVQVLRGEALLD"
     gene            complement(3850..4749)
                     /locus_tag="AC16HB_14565"
     CDS             complement(3850..4749)
                     /locus_tag="AC16HB_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5608057.1"
                     /translation="MSAIAVASTSNARASLLLGLAGVLAFSLTLPMTRAAVAELDASF
                     VAFGRMAIAGLVAAAWLAATRTRVPARAQWARLAWASLGIVFGFPLLSSLAMRSMPAS
                     HGAIVNGLLPFTTALFAAWLYHDRQGRRFWVSAAIGSAIVIAFALREGNATLTAGDLW
                     MLGAVVLGALGYVIGGRLAAAIGGIDVILWALVIALPISAPIALAFAFTTKLDASAAA
                     WGGFVYVALVSQLIGFFAWYNGLALGGIARVGQVQLLQVFFTIAFAALAFGERVEPMT
                     WLAAAAVVVCIAIGRSGPRQEQR"
     gene            complement(4746..>4942)
                     /locus_tag="AC16HB_14570"
     CDS             complement(4746..>4942)
                     /locus_tag="AC16HB_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5608058.1"
                     /translation="DAHLSTRRFLTGDEFTMADIPVGCQIHRWFGLPIERPALPHLER
                     WYREVSTRASARSVLTRPIT"
BASE COUNT          822 a         1658 c         1733 g          729 t
ORIGIN      
        1 ctcgaagcgc agcgcgacga gagcggcaag gacgcggcgg tgcggctcgt gttcgagccc
       61 agaacctcga agatcgaccg cgacgaattc gtcaacgtcc tgctcgcgca cacgagcctc
      121 gaaacgagcg tcccgatcaa cctggtgatg atcggccgcg acggcaagcc gcggtgcaag
      181 acgctgcgcg agatcctcgc cgagtgggtc gagttccgcg tcgagaccgt gcgccagcgc
      241 acgcagcatc ggttggcgaa ggtcgaggac cgcatccaca tcctcgaggg ccggcaggcg
      301 gtcctgctga acatcgacaa agtgatcaag ctgatccgca actcggacga gccgaagccc
      361 gacctgatca aggcgttcaa gctgaccgaa cggcaggccg acgacatcct cgacctgcgg
      421 ctgcggcagc tggcgcggct cgaaggcatc aagatcgagc aggagcttgg cgagctgcgc
      481 aaggaccagg ccgggctgca gaaactgctc ggttccgagg ccgcgctgcg caagcaggtg
      541 gggcgggagt tcgacgagga cgccaagaag tacggcgacg cccgccgcac gctgatcgag
      601 gcggccgagc gcgcgagcgt ggagaccaag gtcgtcgacg agcccgtcac ggtgatcatc
      661 tccgagcagg gtttcgtgcg tgcgcgcacc ggccacggtc acgacaagac gcaattcacg
      721 ttcaagccgg gcgacgcgct gtacgacgcg gtcgagtgcc gcacggtcga tcagctgatc
      781 gcgatcggct ccaacggccg cgtgtacacg gtgccgatcg cgcagctgcc gtcggcgcgc
      841 ggggacggcg cgccagtcac gtcgctgatc gagctggagg cgggcacgcg cgtcgtcgcc
      901 ttctgcgccg gcgccgtgac gcaaccgctc ttcatgtcga ccagcagcgg ccacggcttt
      961 gcatgccagc tgggcgacat ggtgacgcgg cagaagggcg gcaagcagtt catcaccgtc
     1021 gacgaaggcg cggtgccgct gcggccggcg ggcgtcgatc ccgcgacaga cgaccgcatc
     1081 gcatgcctgt cggccaacgg ccgtctgctg gtattcgcgg cgaatgaggt caagatgcaa
     1141 tccggtggcg gccgcggtgt gacgctgatg ggcctggact ccggcgaacc gctgcttgcc
     1201 gcgctgtcgt gctcgacggc gggagtgatc gtgtcgggca cgggcaagag cggcaagccg
     1261 gtggaagtcg cggttgccgg cgccgatctc gccgcgcact ctggccatcg cgcgcgcaag
     1321 ggcacgctgg tgaagtcaaa gctcaaggtg agcggactgc ggcgcgcgtg aaccgatgaa
     1381 gcgccgctct ctggtcgccg tggtggcggc ggtcgcggcc gcgggtctgt tcgtcgcggt
     1441 cgccggcgtt ccggggtgcg tgaccgtgaa tccgtactac gcgcaatcga aggcgcatca
     1501 ccggcccgat ggattccaga acatcgatcc ggcggcggcg atgccgcgcc cgttcagcga
     1561 attccttcgt tggcaggccg agcgcatgac gctgacgatt tccgaaccga agctcgatct
     1621 gtcgccgctc gttccggacc tcggctacat tggaaagaac cgcaccgagc tggcggtcac
     1681 gtggatcggg cacgcaacca cgctgctgca gatcggcggc atcaacgttc tgaccgatcc
     1741 gattttctcc gagcgtgcat cgccggtgca gtggatggga ccgaagcggc gccagccgcc
     1801 cggcgtgctg ctgcgcgagt tgccgcatat cgacgtcgtg ctgatctcgc acaaccacta
     1861 cgaccatctc gacgaggctt cggtcaaggc gctcaatgcg caggcggccg ggccgccgct
     1921 cttcgtggtg ccgctcggcg tggagcggtg gttcgctgac gcaggcatca ccaacaccaa
     1981 ggcgctcgac tggtgggacg cgatcgatgt ccgcggcacg cgcgtgcacc tggttcccgt
     2041 tcagcactgg agcggccgcg ctttcgccga ccggatgaag acgctgtggg gtggctacgt
     2101 gatcgagggc ggcggcaagc gcgcgttctt caccggcgac accggctact cgtccgctca
     2161 cttcagggag atcggcgagc ggctcggccc gatcgatctg gcgctgattc cgatcggcgg
     2221 ctacgagcca cgctggttca tggcgaagca gcacgtgaac ccggaggaag cggtgcagat
     2281 cgcggtcgac ctgaaggcgc ggcgcgcgct cggaatccac tggggcacat ttcagctgac
     2341 cgacgaaccg ctcgaccagg cggtcgcgga tctcgcggcc gcgctgaaga agcacggcct
     2401 caccgagcgg caattcttcg tgttgcggca tggcgagacg cggcgcgtgc tcgactaggg
     2461 cttgggaaga catcatgaaa acgtatgcgc tgatgctgtt cctgcacctt gtggcgatcg
     2521 tcatctggat cggcggcatg ttcctgatgc agttcgcggt gcggccgtcc gcggtgcagt
     2581 tactggaacc gccgcagcgg ctgccgctgc ttgccgagat cctgcgccgc ttcttcaact
     2641 gggtcgcggc cgccgcagtc gtggtgctgc tgtccggcat ctcgatcatt ctcgttgccg
     2701 gcggcttcaa ggtcgtgcac ccgtcggtgc acctgatgtt cggcatcggc gtggtgatga
     2761 tcgcgatctt cctgcacatc cgcctggcgc cgtttcctcg cctgcgggcc gccgtcgcgg
     2821 cgcgcgactg gcccgcgggc gccatgtcgc tcgatcagat ccgcaaggag gtcgcgttca
     2881 atctcgtgct tggcgtggcg atcatcgcga ttgccacgct cgggcgcgtg ctgctgtagc
     2941 gaacgcgtca gtcgaggagc gcctcgccgc gcaacacctg cacggcgccg ccgcccacgg
     3001 tggcgcggat gccgtcgctg ccgcgacgtg ccgacgcgag caaccgactc ggccgtccca
     3061 tctcgacgcc ctgcgcgatc tcgaactgtg cctgttcccc ggttccgagc gacagcagga
     3121 acgcggcgag cgccgcattg gcgctgccgg tggcgggatc ctcgaccgtg ccggacagcg
     3181 gcgcgaacat gcgcgcgcgc agtcgattcg gggttgcgcc ttcagcgcgt gcgtacagat
     3241 gcagcgacag gcgatcgggc ggttcgtgga agctctgcgc gaggcggcgg aacgcgccga
     3301 cgtccggcac cgcgcgcgtc agcgcttcgg cagtgacctc ggcaaaacag aagggcaggc
     3361 cgaccgacgc gatgcgcggc ggatgcgtgc gcaccacgag gtcgcccgcg ctgaggcctg
     3421 cgcaggcggc gaccgcatcg acctgcaggt ccgggccggt cgacagcggc tgcggtgcgg
     3481 cgatatgcgc gccggcgatc gccccgcctg catcgcgggc gatgcgcacc tcgaccagcc
     3541 cggcgatctc ctcgaagcgc agcacgtcgc cgctcgcgcg ccccattccc gcgagcacgc
     3601 agccggtgcc gacgttcgga tgcccggcga acggcatctc ggacgtgcga ttgaatatgc
     3661 ggacgcgcgc cgtgttcgtc ggatcggacg gcggcagcac gaacgtcgtc tcgctgtagt
     3721 tcatttcggc cgcgagcgac tgcatttcgc ggtcggacaa atcgcgcgcg tcggtgaata
     3781 ctgccagcgg gttgccaccg aagcgctggt gggtgaacac atcgaccgtc acgtaactgg
     3841 cactgcgcgt catcgttgct cctgtctggg tcccgaccgt ccgatcgcga tgcacacgac
     3901 caccgcggcg gcagcgagcc acgtcatggg ttcgacgcgt tcgccgaacg caagcgcggc
     3961 gaacgcgatc gtgaagaaca cctgcagcag ctgcacctgc ccgacgcgcg cgatgccgcc
     4021 gagcgcaagc ccgttgtacc aggcgaagaa gccgatcagc tgcgaaacca gcgcgacgta
     4081 gacgaatccg ccccacgcgg ccgcgcttgc gtcgagcttc gtggtgaacg cgaacgcgag
     4141 cgcgatcgga gccgagatcg gcaatgcgat gacgagtgcc cacaggatca cgtcgatgcc
     4201 gccgatggcg gccgcaagcc ggccgccgat cacgtagccg agcgcgccga gcacgaccgc
     4261 accgagcatc cacaggtcgc ccgcagtcag cgtcgcgttg ccttcgcgca gcgcgaaggc
     4321 gatcacgatc gccgagccga tcgccgcgct gacccagaag cgccgaccct ggcggtcgtg
     4381 gtacagccac gcggcaaaga gggcggtggt gaacggcagc agcccgttga cgatcgcccc
     4441 gtggctcgcc ggcatcgagc gcatcgccag cgacgacagc agcggaaagc cgaacacgat
     4501 gccgagcgac gcccacgcga gccgcgccca ctgcgcgcgc gctggcacgc gcgtgcgcgt
     4561 ggctgcgagc cacgcggcgg cgacgaggcc ggcgatcgcc atgcggccga aggcgacgaa
     4621 cgacgcgtcg agttcggcga ccgcggcgcg cgtcatcggc agcgtcaggc tgaacgccag
     4681 cacgccggcc agacccagca gcaggctcgc acgcgcgttg ctggtcgacg cgaccgcgat
     4741 ggcactcatg taatcggcct ggtgagcacg ctgcgcgccg aagcgcgcgt cgacacctcg
     4801 cggtaccagc gctccaggtg cggcagcgcc gggcgctcga tcggaaggcc gaaccagcga
     4861 tgaatctggc agccgaccgg aatgtcggcc atcgtgaact cgtcaccggt caggaagcgg
     4921 cgcgtggaca gatgcgcatc ca
//
LOCUS       DBNHBY010000602         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_312173, whole genome shotgun sequence.
ACCESSION   DBNHBY010000602 DBNHBY010000000
VERSION     DBNHBY010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_312173"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="AC16HB_14575"
     CDS             complement(<1..848)
                     /locus_tag="AC16HB_14575"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR5608059.1"
                     /translation="MDAILSARRRALLVGALGAAALAGCGRAARDELAFSGETMGTNY
                     HVKLAHARLGPAQRDTLHAAVRAAFERVERGMSLYREDSELVRFNRHRSESPFALSRD
                     LHDVLAAALEVSALSGGAFDVTVAPLVASLGFGPDGQRTVPEPTELLARRQAVGYRAL
                     RLDAAARSATKAHASLSADLNGIAKGYGVDVAAQALEAHGIANYLVEAGGEVRSRGVN
                     ADGRAWQIGIEQPDALPPRARYIAPLAHGAMATSGDYRIYFERAGRRYCHEIDPARAA
                     PITHGLA"
     gene            complement(857..1306)
                     /locus_tag="AC16HB_14580"
     CDS             complement(857..1306)
                     /locus_tag="AC16HB_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5608060.1"
                     /translation="MSNLTIESVVHDKGGDHMFVIPAAASVAEAVDMMVANEIGAVLV
                     MTEDGLVGGIFTERDLLVRVVKPGLDPNRTPISLVMTRDVRFVSPGTTIEAALALMHV
                     QRHRHLLVMDGPRVHGLVSMRDLAYHLICHGEGRFEAALRSATAKDE"
     gene            complement(1316..2539)
                     /gene="nqrF"
                     /locus_tag="AC16HB_14585"
     CDS             complement(1316..2539)
                     /gene="nqrF"
                     /locus_tag="AC16HB_14585"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HIR5608061.1"
                     /translation="MSEILLGVVMFTGVILALVALLLGAKRKLVASGDVSIVINDDTS
                     KALRVPAGGSLLAALADNRIFIPSACGGKGACGVCEVVVREGGGDLLPTETGYITPGE
                     ARRGHRLACQVKVKRDLRIEVAPEVFNVRKWKCRVVSNRNVATFIKELKLALPEGEEV
                     PFRAGGYVQIECPPHRLRFKSMGIDPQYRDVWDKFDLWRFASGTDTPVTRAYSMANYP
                     LEKGLLLFTIRIAFPPDYREDIPPGIMSSWVFNLKPGDEVTVSGPFGEFFARDTEAEM
                     CFVGGGAGMAPMRSHIFDQFHRLHTRRKATFWYGARSLREAFYVDEFDRLQAEHRNFT
                     WHLALSEPLPEDDWKGPVGFIHQVLYDRYLKDHPAPEDIEYYMCGPGPMTKAVIAMLL
                     ELGVDRDNILLDDFG"
     gene            complement(2595..>3046)
                     /locus_tag="AC16HB_14590"
     CDS             complement(2595..>3046)
                     /locus_tag="AC16HB_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR5608062.1"
                     /translation="NQVILKQLLGEGALAWLHPTLASVDLTFLAFILFIGTIAAATQV
                     VEMAVDRFAPKLYATLGVFLPLIAVNCVILAGSLFMQERDYTLAESVVYGVGSGLGWA
                     LAIVAMAAVREKLAYSNVPAGLKGLGITFITAGLMSLAFMMFSGIQL"
BASE COUNT          503 a         1074 c          993 g          476 t
ORIGIN      
        1 gccagtccgt gcgtgatcgg cgcggcgcgt gccggatcga tctcgtggca gtagcgcctg
       61 ccggcgcgct cgaaatagat gcggtaatcg cccgaggtcg ccatcgcgcc atgcgcgagc
      121 ggcgcgatgt agcgtgcgcg cggcggcagc gcgtccggct gctcgatgcc gatctgccac
      181 gcgcgcccgt cggcgttgac gccgcgcgac cggacttcgc cgccggcctc gaccaggtaa
      241 ttggcgatcc cgtgcgcttc gagcgcctgc gcggcgacat ccacgccgta gcccttcgcg
      301 atgccgttca gatccgcgct aagcgacgcg tgcgccttgg tcgcgctgcg cgccgccgcg
      361 tcgaggcgta acgcccggta gccaacggcc tgccggcgcg caagaagctc ggtgggctcg
      421 gggacggtac gctgcccgtc cggcccgaac cccaagctcg ccaccagcgg cgcgaccgtg
      481 acgtcgaacg caccgccgga cagcgcgctc acctccagcg cggccgcgag cacgtcgtgc
      541 aggtcgcgcg acagtgcgaa cggcgactcg gaccgatggc ggttgaagcg caccagttcc
      601 gagtcctcgc gatacagcga catgccgcgc tcgacgcgct cgaacgccgc gcgcacggct
      661 gcgtgcagcg tgtcccgctg cgcggggccg aggcgcgcgt gcgccaactt gacgtggtag
      721 ttcgtgccca tcgtctcgcc gctgaaggcg agttcgtcgc gtgcggcgcg gccgcagcct
      781 gccagcgccg cggcgccgag cgcacccacc agcagcgcgc gccggcgagc ggaaaggatt
      841 gcatccatgg cgagcctcat tcgtccttgg cagtggcgga acgcagcgcc gcctcgaagc
      901 gcccttcgcc gtggcagatc aggtggtacg ccagatcgcg catcgagacc aggccgtgca
      961 cgcgcgggcc atccatcacc agcaggtggc gatgccgctg cacgtgcatc aatgccagtg
     1021 ccgcttcgat cgtggtgcca ggcgatacga atctcacgtc gcgcgtcatc acgagcgaaa
     1081 tgggcgtcct gttcggatcg agccccggct tgacgacgcg caccagcagg tcgcgctcgg
     1141 tgaaaatgcc gccgaccagc ccgtcctcgg tcatcaccag caccgcgccg atctcgttgg
     1201 cgaccatcat gtcgaccgcc tcggcgacgc tcgcggcggc cgggatcacg aacatgtggt
     1261 cgccaccctt gtcgtgcacc acgctttcga tcgtcaggtt cgacatgcgg cctcctcagc
     1321 caaagtcgtc gagcaggatg ttgtcacgat ccacgccgag ttcgagcagc atcgcaatga
     1381 cggcttttgt catggggccg ggcccgcaca tgtagtactc gatgtcctcg ggcgcggggt
     1441 gatccttgag ataccggtcg tacagcacct ggtggatgaa gccgaccgga cccttccagt
     1501 cgtcttccgg cagcggctcc gagagcgcca gatgccaggt gaaattcctg tgctcggctt
     1561 gcagccggtc gaactcgtcg acgtagaacg cctctctcag gctgcgcgcg ccgtaccaga
     1621 acgtcgcctt gcgccgcgtg tgcagccggt ggaactggtc gaagatgtgc gagcgcatcg
     1681 gcgccatgcc ggcgccgccg cccacgaagc acatctcggc ttcggtgtcg cgcgcgaaga
     1741 actcaccgaa cgggccggat accgtcacct cgtcgcccgg tttcaggttg aacacccacg
     1801 aactcatgat ccccggcggg atgtcctcgc ggtaatcggg cggaaacgcg atgcggatcg
     1861 tgaacagcag gaggcccttt tccagcgggt agttcgccat cgagtaggcg cgtgtcaccg
     1921 gcgtgtccgt gcccgacgcg aagcgccaca ggtcgaactt gtcccacacg tcacggtact
     1981 gcggatcgat gcccatcgac ttgaagcgca accggtgcgg cggacattcg atctgcacgt
     2041 agccgcccgc gcggaacggt acctcttcgc cctccggcag cgcgagcttc agttccttga
     2101 tgaaggtcgc cacgttgcgg ttgctgacca cgcggcactt ccacttgcgc acgttgaaga
     2161 cttccggcgc gacctcgatc ctgagatccc gcttgacctt gacctggcag gcgagccgat
     2221 ggccgcggcg cgcttcgccc ggcgtgatgt agccggtctc ggtcggcagc aggtcgccgc
     2281 cgccttcacg caccaccact tcgcatacgc cgcatgcgcc cttgccgccg cacgccgacg
     2341 gaatgaagat ccggttgtcg gccagcgcgg ccagaagaga gccgccggcc ggcacgcgca
     2401 acgccttcga cgtgtcgtcg ttgatcacga tcgacacgtc gccgctggcg accagtttgc
     2461 gcttggcacc gagcagcagc gccaccagcg ccaggatcac gccggtgaac atcaccacgc
     2521 cgagcaggat ttcgctcatg gagccagcct tccgcgtcgc ttcgggctcg agcgctcgag
     2581 acggcccggc gcgctcatag ctggatgccc gagaacatca tgaacgccag cgacatcagc
     2641 ccggcggtga tgaacgtgat gccgagtccc ttgaggcccg ccggcacgtt cgagtaggcc
     2701 agcttttcgc gtaccgccgc catcgcgacg atcgcgagcg cccagccgag ccccgagccg
     2761 acgccgtaaa ccacgctctc ggccagcgtg tagtcgcgct cctgcatgaa gagcgaaccg
     2821 gccaggatca cgcagttcac cgcgatcagc ggcaggaaca cgccgagcgt ggcatacagc
     2881 ttcggcgcga agcggtcgac cgccatctcg accacctgcg tcgcggcggc gatcgtgccg
     2941 atgaacagga tgaaggcgag gaacgtcagg tcgacgctcg ccagcgtcgg atgcagccat
     3001 gccagcgcgc cctcgccgag cagctgcttc aggatgacct ggttca
//
LOCUS       DBNHBY010000603        10639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1800399, whole genome shotgun sequence.
ACCESSION   DBNHBY010000603 DBNHBY010000000
VERSION     DBNHBY010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1800399"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /gene="moaC"
                     /locus_tag="AC16HB_14595"
     CDS             complement(<1..468)
                     /gene="moaC"
                     /locus_tag="AC16HB_14595"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5608063.1"
                     /translation="MTSLTHFDDAGQAHMVDVGAKAVTHRVAVAAGTIRMQPLTLDLI
                     RSGTSKKGDVLGVARIAAIQAAKKTSELIPLCHPLAITRIAVDFHLDEAASRVECRAQ
                     VETHGQTGVEMEALTAVQVGLLTIYDMCKAVDRGMRIENLHLLEKSGGKSGHWV"
     gene            557..2107
                     /locus_tag="AC16HB_14600"
     CDS             557..2107
                     /locus_tag="AC16HB_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5608064.1"
                     /translation="MPKFAARLVSLAVAATLTAQPALIPVVRAQSQDLPALGEAGADD
                     LSPGNERRLGEAIMREARRDPSYMDDPDATEYLNNLGFKLVAASPARYTDFGFFCVRD
                     PMLNAFALPGGFIGVHSGLILTAQSESELAAVMAHEIGHVAQRHIARMIAKQRDSGLI
                     AIGALLLAILAARSGSSSSGQATEAALAVGQAAAIQQQLNFSREAEREADRVGFQILN
                     DAGFDVTAMASFFTRLQQGSRIYESAAPAYLRTHPLTVERIGDIQTRVRELKPHPRPD
                     RLEFQLVRARLRVLQDDSLQGAHDALDYFTGQLKNRSTPSEAATHYGAALASLKLNQN
                     GPALEYAQTARKLARVPSAMLDKIVADARYAAAKTDGERAEAVRFARDATLRFPISRL
                     TAMNYVDLLQKSGQHEAAVAFLREQLALPRSEPQYYELLARSYAALDRKTLQHQAIAE
                     MYLLQGSLPAAIDQLQSARKFADADFYVLSEVDARLRQLTQKMREQREELAKSGRTPP
                     PDDEKKRR"
     gene            2241..3335
                     /locus_tag="AC16HB_14605"
     CDS             2241..3335
                     /locus_tag="AC16HB_14605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5608065.1"
                     /translation="MDATVKEPRAEIPGHLEVKPPIETDAVIVGAGPVGLFQVFELGL
                     LEIKAHVIDSLAYVGGQCVELYPDKPIYDIPAVPVCTGQELTDSLLKQIAPFGAAFHL
                     GQEVTHLQRQDDGRFYVQTTLGNSFLTRTVFIAAGVGSFQPRTLKVDGIEQFEGRQLF
                     YRVKDPIQFHGRNLVICGGGDSALDWALALAGKAESLILVHRRDGFRAAPASVAKMRQ
                     LCDELEMQFLIGQVTGYDEAHDRMTAVRVTSLDGVTRKVPLDALLVFFGLSPKLGPIA
                     HWGLDIERKQVVVDTEKFETSVPGIFAIGDINTYPGKKKLILSGFHEAALAAFGAARY
                     VFPEKKIHLQYTTTSPKLHKVLGVETPVFD"
     gene            complement(3332..3904)
                     /locus_tag="AC16HB_14610"
     CDS             complement(3332..3904)
                     /locus_tag="AC16HB_14610"
                     /inference="COORDINATES: protein motif:HMM:NF022609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HIR5608066.1"
                     /translation="MDASVIAAIARWPAVPAVFGWLRLTARGEWRLRGEPIGNAAIRA
                     FIGRNYAPDERGRWFFQNGPQRVYVELEATPWIVRIDAQGALAMHTGALPATCRGAAL
                     LDDGRFVLDTDLGAALVDDRDAATFMGALTDPFGMPLNERGMERWLDGKDEAFVVPAR
                     LCSAGAPCRIERVRVADLEARYGFVRSPAP"
     gene            complement(3915..4808)
                     /locus_tag="AC16HB_14615"
     CDS             complement(3915..4808)
                     /locus_tag="AC16HB_14615"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5608067.1"
                     /translation="MGAGLLQSSIRSLPLAYRGKVRDSYALGGDKLLIVTTDRLSAFD
                     VVMNQPIPDKGRVLNAMSNFWFDKLAAVVPNHLTGIAPESVVAADEVEQVRGRAVVAT
                     KLRPLPVEAVVRGYLIGSGWKDYQRTGSVCGIGLPAGLQMAQKLPQPIFTPATKAELG
                     THDENIPFARMQQIVGAALAEEIQLISLKLYQTAADYAATRGILIADTKFEFGLDSRG
                     TLRLMDEVLTADSSRFWPADDYRAGVSPPSFDKQFVRDWLEAQPWNKAPPPPDLPAEI
                     IEKTAAKYREALRRLTGRDLD"
     gene            4911..6944
                     /locus_tag="AC16HB_14620"
     CDS             4911..6944
                     /locus_tag="AC16HB_14620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5608068.1"
                     /translation="MALDSPPARIDPLAQLNPHQRAAVEHGAEPLLVIAGAGCGKTNT
                     LAHRVAHLIRRGADPRAILLLTFSRRAAVEMTRRVERIAGGQAKGLEWAGTFHAIGAR
                     LIREYAARIGLNPAFTIHDREDSADLMALARHELDLTSTDKRFPLKGTCLAIYSRVVN
                     TEAPLAEVLARSFAWCADWEAELKRLFAAYVAAKQKQNVLDYDDLLLYWASMLQEPAL
                     AAEIAARFAHVLVDEYQDTNRLQASVLLRLAPDGHGLTVVGDDAQSIYAFRGATVRNI
                     LEFPQRFAPPATIVTLEQNYRSTQSILDAANAVIGLAAERFTKNLFTGRGTGARPRLV
                     TVADEAAQARYVVDHVLAQREQGTRLRAQAVLFRASHHSLPLELELARRNVPFVKYGG
                     LKFLEAAHVKDLLACLRWAGNPRDRVAGFRVLLLLPGVGPKTAARALDAIMAAADPID
                     AIARFAPPAACADDWRELAQFAGTRTAWPAEFALARAWYEPHLARLHDSAEARRADLI
                     ALENLAGAYPSRERFLTELTLDPPDATSDEAGPPLADEDYLILSTIHSAKGQEWSAVH
                     VLNAVDGCIPSDLATGTPQEIEEERRLLYVALTRAKDSLHVLVPQRFYVHQQSAGGDR
                     HVYASRTRFIPDALQPFFETSAWPVEGRGREGLVSASPQPAIDLAARMRAMWS"
     gene            complement(6957..7637)
                     /locus_tag="AC16HB_14625"
     CDS             complement(6957..7637)
                     /locus_tag="AC16HB_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR5608069.1"
                     /translation="MSTSADPRRALLAGATGLVGRALLTQLRESARYRQVHVLLRRTA
                     PDLPADPKLNVLLIDFEHLPRLPRTDDVFIALGTTIKVAGSEAAFRHVDFDAVLAVAR
                     AARAAGARRLLVVSALGANAGSRVFYSRVKGEMEQAVAGLGYESLVIARPSLLLGDRA
                     ALGQRVRPGEQWAARLLRPVQKFVPRSVRPIDADTVAASMLSAALHAAPGVTVLGARE
                     LHSMSAGS"
     gene            complement(7726..8643)
                     /locus_tag="AC16HB_14630"
     CDS             complement(7726..8643)
                     /locus_tag="AC16HB_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5608070.1"
                     /translation="MTNPLTAAPFHAGELAVQARAGSRDRMAQLGPRVIREAMPLQHR
                     EFFPLLPFVVLGAVDGAGRPWATLLAGSAAGFVTAPDDTHLRIDALPPAGDPVRGLLQ
                     PGAPLALLGIELPTRRRNRANGRVESRGEHGFVLHVTQSFGNCPKYIRQRELTASVEN
                     VAAAARHGDRFDAGTTASVFAADTFFIASHAAGDAPGTGADVSHRGGPPGFVRVSDDG
                     RTLTWPDFPGNTYFNTLGNLAVEPRAGLVFPDFANGDLLHVAGRAEIVWDGPELAATA
                     GAERLVRLHVEQVLLRPAALPLRWRRIAP"
     gene            complement(8651..9274)
                     /locus_tag="AC16HB_14635"
     CDS             complement(8651..9274)
                     /locus_tag="AC16HB_14635"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5608071.1"
                     /translation="MPIRLYRHPLSGHCHRVELFLRLLGLPFESVDVDLARREHKTPD
                     FLRLNAFGQVPVLVDGDAMIADSNAILTYLALRYADATWLPRDPVGAARVQRWLSAAA
                     GPLAFGAAAARAVQLFRRPDDSRDMIARAHALLAVMEQGLSADGTNFIAGAQPTIADL
                     ALYSYTAHAPEGNVSLDAYPAVRSWLARIEALPGFAPMQASRIGLAA"
     gene            9380..10285
                     /locus_tag="AC16HB_14640"
     CDS             9380..10285
                     /locus_tag="AC16HB_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5608072.1"
                     /translation="MDKFRALQTLVAIVDRGSLTAAADALDASLPAVVRSLAALEAQL
                     GVRLLNRTTRRQSLTEDGRQYLAHARRILADLDEADRSLAARQSEPSGALAVTAPVLF
                     GQYHVAPAITHFLKRYPKMRISLALLDRVVNLVEEGLDVGVRIGHLQDSSVVARPVGR
                     IRRVVVASPALLRRVGTPKHPSELARLPCLHFSATDGSRWAFRHAGRTVTVDVQGSFE
                     CNLAAPIMDACAAGLGFGRCLSYQAAPLLADRHLRIVLADYEIEPWPVHLTYPSARLL
                     PARSKVFIEWMIEALKASLQRTVPV"
     gene            10473..>10639
                     /locus_tag="AC16HB_14645"
     CDS             10473..>10639
                     /locus_tag="AC16HB_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5608073.1"
                     /translation="MIELNVNGARHRVDVEPEMPLLWVLRDHLNLHGTKYGCGISVCG
                     ACTVHVDDAAVR"
BASE COUNT         1670 a         3742 c         3644 g         1583 t
ORIGIN      
        1 aacccagtgc cctgatttgc ctcccgattt ttcgagcagg tgcagattct cgatgcgcat
       61 gccgcgatcg accgccttgc acatgtcgta gatcgtcagc agccctactt gcaccgccgt
      121 cagcgcctcc atctcgactc cggtctggcc atgcgtctcg acctgcgccc ggcattcgac
      181 gcggcttgcc gcttcgtcga ggtggaagtc gaccgcgatg cgcgtgatcg ccagcggatg
      241 gcacagcggg atcagctcgg aggtcttctt cgcggcctgg atcgcggcga tgcgcgcaac
      301 gccaagcacg tcgcccttct tcgacgtgcc gctgcggatg agatccagcg tcagcggctg
      361 catgcggatc gtgcccgcgg ccacggccac gcggtgcgtg accgccttgg cgccgacatc
      421 gaccatgtgc gcctggcccg cgtcatcgaa atgcgtgaga gatgtcattt tccgttacaa
      481 ccgtgtcccg ggaacgatcc cgccggctga ctatcatacg agtcggatct ccgccgcgcc
      541 gaccgccgct agcttcgtgc cgaaattcgc cgcccgcctc gtctcgctcg ccgtggccgc
      601 cacgctgacg gcgcagcccg cgctgatacc cgtcgtacgc gcgcaatcgc aggatctgcc
      661 ggcgctcggc gaggccggcg cggacgacct gtcgccgggc aacgagcggc ggctgggcga
      721 ggcgatcatg cgcgaggcgc gccgcgatcc gtcgtacatg gacgatccgg atgcgaccga
      781 gtacctgaac aacctcggct tcaagctggt tgcggccagc cccgcgcgct acaccgattt
      841 cggcttcttc tgcgtgcgcg acccgatgtt gaatgcattc gcgctgccgg gcgggttcat
      901 cggcgtgcac agcggcctga tcctgacggc gcagagcgaa tccgagctgg ccgcggtgat
      961 ggcgcacgag atcggccacg tcgcgcagcg gcacatcgcc cgcatgatcg ccaagcagcg
     1021 cgatagcggg ctgatcgcga tcggcgcgct gctgctcgcg atcctcgccg cgcgcagcgg
     1081 ctcctccagc agcggccagg cgaccgaggc tgcgctggcg gtgggccagg ccgcggcgat
     1141 ccagcagcaa ctgaacttct cgcgcgaggc ggagcgcgag gccgaccgcg tcggcttcca
     1201 gatcctgaac gacgccggct tcgacgtcac agcgatggca tcgttcttca cccgcctgca
     1261 gcagggttcg agaatctacg agagcgccgc gccggcctac ctgcgcacgc acccgctgac
     1321 ggtggagcgc atcggcgaca tccagacccg tgtgcgcgaa ctgaagccgc acccgcggcc
     1381 cgacagactc gaattccagc tcgtgcgcgc gcgcctgcgc gtgctgcagg acgattcctt
     1441 gcagggcgcg cacgacgcgc tcgactactt caccggccag ctgaagaatc gcagcacgcc
     1501 atcggaggcc gccacccact acggcgccgc gctggcgtcg ctgaagctga atcagaacgg
     1561 tcccgcgctc gaatacgcgc agaccgcgcg caagctcgcg cgcgtgccgt cggcgatgct
     1621 cgacaagatc gtcgccgacg cgcgctatgc cgcggcgaag accgatggcg agcgcgccga
     1681 agccgtgcgt ttcgcgcgcg acgcgacgtt gcgctttccg atctcgcggc tgacggcgat
     1741 gaactatgtc gatctgctgc agaagagcgg ccagcatgag gcggcggtcg cgttcctgcg
     1801 cgagcagctg gcgctgccgc gcagcgagcc gcagtactac gagctgctcg cgcgcagcta
     1861 cgccgcgctc gaccgcaaga cgctgcagca ccaggccatc gccgagatgt acctgctgca
     1921 gggctcgctg cccgccgcga tcgaccagtt gcagtcggcg cgcaagttcg cggacgccga
     1981 cttctacgtg ctgtccgaag tcgatgcgcg cctgcgccag ctgacgcaga agatgcgcga
     2041 acagcgcgag gaactcgcca agagcggccg cacgccgccg ccggatgacg agaagaagcg
     2101 gcgatagccc gctgcgcgcg cgggtttccg ccgccgtcaa acggaaatat gcttagagcc
     2161 cgtcgtcggg tgcgcgcgcc ccggatcggc gaaaatcacg gctgcacgac gaaccgcgcg
     2221 cgctgccgcg ccgccgctcc atggacgcca ctgtgaaaga acccagggcc gagatccccg
     2281 gccacctcga agtcaagccg ccgatcgaga ccgacgccgt gatcgtcggc gccggtcccg
     2341 tcggcctgtt ccaggtcttc gagctcggcc tgctcgagat caaggcgcac gtgatcgatt
     2401 cgctggccta cgtgggcgga cagtgcgtcg agctgtatcc cgacaagccg atctacgaca
     2461 ttccggcggt gccggtgtgc accggccagg agctgaccga cagtctgctg aagcagatcg
     2521 cgccgttcgg cgccgcgttt cacctcggcc aggaagtcac gcacctgcag cggcaggacg
     2581 acggccgctt ctacgtgcag accacgctcg gtaacagttt cctgacgcgg accgtgttca
     2641 tcgcggccgg cgtcggcagc ttccagccgc gcacgctgaa ggtcgacggc atcgagcagt
     2701 tcgagggccg gcagctcttc taccgcgtga aggacccgat ccagttccac ggcaggaacc
     2761 tcgtgatctg cggcggcggc gactccgcgc tcgactgggc cctggcgctc gccggcaagg
     2821 ccgaaagcct gatcctggtg catcgacgcg acggcttccg cgccgcgccg gcatcggtcg
     2881 cgaagatgcg ccagttgtgc gacgaactcg agatgcagtt cctgatcggc caggtgaccg
     2941 gctacgacga ggcgcacgac cgcatgaccg ccgtgcgcgt gacctcgctc gatggcgtca
     3001 cgcgcaaggt gccgctcgat gcgctgctgg tgttcttcgg gttgtcgccg aagctgggtc
     3061 cgatcgcgca ctgggggctc gacatcgagc gcaagcaggt cgtggtcgac accgagaaat
     3121 tcgagaccag cgtgcccggc atcttcgcga tcggcgacat caacacctac ccgggcaaga
     3181 agaagctgat cctgtcgggc ttccacgagg cggcactggc cgcgttcggc gccgcccggt
     3241 acgtgtttcc cgagaagaag atccaccttc aatacacgac gaccagtccc aagctgcaca
     3301 aggtgctcgg ggtcgagacg ccggtgttcg actagggcgc gggcgagcgc acgaagccgt
     3361 agcgcgcttc gaggtccgcg acgcgcaccc gctcgatgcg gcacggcgcg ccggcggagc
     3421 acaaccgcgc gggcacgacg aacgcctcgt ccttgccgtc gagccagcgc tccatcccgc
     3481 gctcgttcag cggcattccg aagggatcgg tgagcgcgcc catgaaggtt gccgcgtcgc
     3541 ggtcgtccac cagcgctgcc cccaaatcgg tgtcgagcac gaagcggccg tcgtcgagca
     3601 gggcggcgcc acggcaggtg gcgggcagcg cgcccgtgtg catcgcgagc gcgccttgcg
     3661 catcgatgcg aacgatccac ggcgttgcct cgagttcgac gtacacgcgc tgcgggccgt
     3721 tctggaagaa ccagcggccg cgttcgtcag gcgcgtagtt gcggccgatg aatgcgcgga
     3781 tcgccgcgtt gccgatcggc tcgccgcgca ggcgccattc gccgcgcgcg gtgagacgca
     3841 gccagccaaa cacggccggc acggccggcc acctggcgat cgcggcgatg acgctcgcgt
     3901 ccatcgccgt agcctcagtc gaggtcgcgc ccagtcagcc gccgcagcgc ctcgcgatac
     3961 ttcgccgcgg ttttctcgat gatctccgcc ggtagatccg gcggcggcgg cgccttgttc
     4021 cacggctgcg cctccagcca gtcgcgcacg aactgcttgt cgaaactcgg cggcgaaacg
     4081 ccggcgcgat agtcatcggc cggccagaag cgcgacgaat ccgcggtcag cacctcgtcc
     4141 atcagccgca atgtgccccg gctgtccaac ccgaactcga acttggtgtc ggcgatcagg
     4201 atgccgcgcg tcgccgcgta gtcggccgcg gtctggtaca gctttaggct gatcagctgg
     4261 atctcctcgg ccagcgcggc gccgacgatc tgctgcatgc gcgcgaacgg aatgttctcg
     4321 tcgtgcgttc cgagttcggc cttggtcgca ggcgtgaaga tcggctgcgg cagcttctgc
     4381 gccatctgca ggcccgcggg caatccgatg ccgcacacgc tgccggtgcg ctggtaatcc
     4441 ttccagcccg agccgatcag gtatccgcgc accaccgcct cgaccggcag cggccgcagc
     4501 ttcgtcgcaa ccacggcgcg tccgcgcacc tgttcgacct catccgcggc aaccacgctc
     4561 tccggcgcga tgccggtcag gtgattcgga accaccgctg ccagcttgtc gaaccagaag
     4621 ttcgacatcg cgttgagcac gcgccccttg tcggggatcg gctggttcat cacgacgtcg
     4681 aatgccgaca ggcgatcggt ggtgacgatc aacagcttgt cgccgccgag cgcgtaggag
     4741 tcgcgcacct tgccgcgata tgccagcggc agcgatctga tcgagctttg cagcaggcct
     4801 gcacccatcg ttccatctcc gggagtagga aagcatctta gtcgagcgtc ctgcgcgccg
     4861 cgggcatggg cactgtatat ttcgccagta cccattagcc ctcgcctgcg atggccctcg
     4921 acagtccgcc cgcgcgcatc gatccgctcg cccaactgaa tccgcaccag cgcgccgcgg
     4981 tcgagcacgg cgccgagccg cttctcgtga tcgcgggcgc gggctgcggg aagacgaaca
     5041 cgctcgcgca ccgggtcgcg cacctgatcc ggcgcggcgc cgatccgcgt gcgatcctgc
     5101 tgctcacctt ctcgcgccgt gcggcggtcg agatgacgcg acgggtggag aggatcgccg
     5161 gcgggcaggc aaaggggctc gagtgggccg gcacgttcca cgcgatcggc gcgcggctga
     5221 tccgggagta cgccgcacgc atcgggctga atcccgcgtt cacgatccac gaccgcgagg
     5281 actccgcaga cctgatggcg ctagcccggc acgaactcga cctcacgtcg accgacaagc
     5341 gcttcccgct gaagggcacc tgcctggcga tctactcgcg cgtggtcaac accgaggcgc
     5401 cgctcgcgga ggtgctggca cggtcgttcg cctggtgcgc ggactgggaa gccgagctga
     5461 agaggctctt cgcggcgtac gtcgcagcca agcagaagca gaacgtgctc gactacgacg
     5521 acctgctgct gtactgggcg tcgatgctgc aggagcccgc gctcgcggcc gagatcgcag
     5581 cgcgtttcgc gcacgtgctg gtcgacgaat accaggacac caatcggctg caggcctcgg
     5641 tgctgctgcg gctcgcgccc gacgggcacg ggctcacggt ggtcggcgac gacgcccagt
     5701 cgatctacgc gttccgtggc gcgacggtgc gcaacattct cgaattcccg caacggttcg
     5761 cgccgcccgc cacgatcgtc acgctcgagc agaactaccg ttcgacgcag tcgatactcg
     5821 acgctgccaa cgcggtgatc gggctggccg cggagcggtt cacgaagaac ctgttcaccg
     5881 ggcgcggcac cggcgcgcga ccacggctcg tgacggtggc cgacgaagcg gcgcaggcgc
     5941 gctatgtggt cgaccacgtg ctggcgcagc gtgagcaagg cacgcgcctg cgcgcgcagg
     6001 cagtgctgtt ccgtgcttcg catcacagcc tgccgctcga actggaactc gcacggcgca
     6061 acgtgccgtt cgtgaagtac ggcgggctga agttcctgga agcggctcac gtgaaggatc
     6121 tgctcgcctg cctgcgctgg gccgggaacc cgcgcgaccg cgtggcgggt tttcgcgtgc
     6181 tgctgctgct gcccggcgtc ggaccgaaga ccgccgcgcg cgcgctggat gcgatcatgg
     6241 cagcagccga cccgatcgat gcgatcgcgc gcttcgcgcc accggccgcg tgcgcggacg
     6301 actggcggga gctcgcgcag ttcgctggca cgcgtaccgc gtggccagcc gagtttgcgc
     6361 tcgcgcgtgc ctggtacgag ccgcacctgg cgcggctgca cgacagcgcg gaggcgcggc
     6421 gcgccgatct gatcgcgctg gagaatctcg ccggtgcgta tccatcgcgc gagcgcttct
     6481 tgaccgagct cacgctcgat ccgcccgacg ccaccagcga cgaggccggc ccgccgcttg
     6541 ccgacgagga ctacctgatc ctgtcgacga tccactcggc caaggggcag gagtggagcg
     6601 ccgtgcacgt gctcaacgcc gtcgacggct gcatcccgtc ggatctggcc accggcacgc
     6661 cgcaggagat cgaggaggaa cgccgcctgc tgtacgtcgc gctgacgcgt gccaaggaca
     6721 gcctccacgt gctggtgccg cagcgcttct acgtgcacca gcagtccgcc ggcggcgacc
     6781 ggcacgtcta cgcatcgcgc acgcgcttca ttccggatgc gctgcaacca tttttcgaga
     6841 cgagcgcgtg gccggtcgaa gggcgcggca gggaaggatt ggtctcggct tcgccgcagc
     6901 cagccatcga cctcgcggcg cgcatgcggg cgatgtggtc ctgaacgcag cgccgttcag
     6961 gagcctgcgc tcatcgagtg cagctcgcgg gcaccgagga ccgtgacgcc cggcgcggcg
     7021 tgcagcgccg ccgacagcat cgacgcggct acggtgtcgg cgtcgatggg gcgcacgctg
     7081 cgcggcacga acttctggac cgggcgcagc agccgcgcgg cccattgctc gcccgggcga
     7141 acgcgctggc cgagcgcggc gcgatcgccg agcaacagcg acggccgtgc gatcacgagg
     7201 ctttcgtagc ccagcccggc cacggcctgc tccatttcgc ccttgacacg gctgtagaag
     7261 acgcgcgagc cggcgttggc gccaagcgcc gacacgacca gcaggcggcg ggcgccggcc
     7321 gcgcgcgccg cgcgcgcgac tgcgagcacg gcgtcgaagt cgacatgccg gaaggctgcc
     7381 tccgatcctg ccaccttgat cgtggtgccg agcgcaatga agacgtcgtc ggtgcgcggc
     7441 agccgcggca gatgctcgaa gtcgatcaac agcacgttca gcttcggatc ggccggcagg
     7501 tcgggcgccg tgcgtcgcag cagcacgtgc acctgccggt agcgcgccga ttcgcgcagt
     7561 tgcgtcagca gcgcgcggcc gacgagaccg gtggctcccg ccagcagcgc gcggcgcggg
     7621 tcggccgagg tcgacatgga ctgattccct gcgcttcgag cagcgcggga tcatgcgcgt
     7681 tggggcgcgc gatgacgatc gcgcaactcc gaccgcgcgt cgcgatcagg gcgcgattcg
     7741 ccgccagcgc agcggcagcg ccgcgggccg cagcagcacc tgctcgacgt gcaaacgcac
     7801 caggcgctcc gcccccgcgg tggccgcgag ttcgggcccg tcccagacga tctcggcacg
     7861 gccggcgacg tgcagcagat cgccattcgc gaaatcgggg aagacaaggc ccgcgcgcgg
     7921 ctcgaccgcg agattgccga gcgtgttgaa gtaggtgttg ccggggaagt cgggccacgt
     7981 cagcgtgcgc ccgtcgtcgc tgacgcgaac gaaacccggc ggaccgccgc gatgggacac
     8041 atcggcgccg gtgcccggtg cgtcgccggc ggcatggctg gcgatgaaga acgtgtcggc
     8101 ggcgaatacg cttgccgtcg tgccggcgtc gaagcggtct ccgtgccgcg ctgcggcggc
     8161 cacgttctcg acgcttgcgg tgagttcgcg ctgccggatg tacttcgggc agttgccgaa
     8221 actctgcgtc acgtgcagca cgaagccgtg ctctccgcgc gactcgacgc ggccgttggc
     8281 gcgattgcgc cgacgcgtcg gcaattcgat tccgagcagt gccagcggag cacccggttg
     8341 cagcagcccc cgcaccggat cgcccgcggg cggcagtgcg tcgatgcgca ggtgcgtgtc
     8401 gtcgggcgcg gtgacgaatc cagccgcgga tcccgcgagc agcgtggccc agggccggcc
     8461 cgcgccgtcc accgcgccga gcacgacgaa cggcagcagc ggaaagaact cgcgatgctg
     8521 caacggcatc gcttcgcgga tcacgcgcgg gcccagctgc gccatcctgt cgcgcgatcc
     8581 ggcgcgggcc tgcaccgcga gttcgccggc atgaaacggc gccgccgtga gcggattcgt
     8641 cacggctgcc tcaggccgcc aggccgattc ggctcgcctg catcggcgcg aagccgggca
     8701 gcgcctcgat gcgcgcgagc cagctgcgca ccgcaggata ggcgtcgagc gacacgttgc
     8761 cctcgggcgc gtgcgcggtg tagctgtaca gcgcgaggtc ggcgatcgtc ggctgcgcgc
     8821 cggcgatgaa gttcgtgcca tcggccgaca gcccctgctc catcacggcg agcagcgcgt
     8881 gtgcgcgcgc gatcatgtcg cgcgagtcgt ccggccgacg aaacagctgg accgcgcgcg
     8941 ccgcggccgc gccgaacgca agcggcccgg ccgccgccga aagccagcgc tgcacccgcg
     9001 ctgcgccgac cggatcgcgc ggcagccacg ttgcgtccgc atagcgcagc gcgagatagg
     9061 tcaggattgc gttggagtcg gcgatcatgg catcgccgtc gaccagcacc ggaacctggc
     9121 cgaacgcgtt gagccgcagg aaatccggcg tcttgtgctc gcggcgcgcg aggtcgacgt
     9181 cgaccgactc gaacggcagc ccgagcaggc gcagaaagag ttcgacgcga tggcaatggc
     9241 cggaaagcgg atggcggtac agcctgatcg gcatgacggg ctcctggcgg gggcgaccgc
     9301 attctgggtc gcggcgctgg tggaataaag acggttttcc ggatttgact gctccatttc
     9361 gtggagcaat catccggcga tggacaagtt ccgcgccttg cagacgctcg tcgccatcgt
     9421 cgaccgcggc agcctgacgg cggccgcgga tgcgctcgat gcatcgctgc ccgccgtggt
     9481 gcgctcgcta gccgcgctcg aagcgcaact cggcgtgcgc ctgctgaatc gcaccacccg
     9541 gcgccagtcg ctgacggagg acggccggca atacctggcg cacgcgcgcc gcatcctcgc
     9601 cgacctcgac gaggccgacc gctcgctcgc cgcgcggcag tcggagccga gtggcgcgct
     9661 cgccgtgacg gcgccggtgc tgttcggcca gtaccacgtc gcgccggcga tcacgcactt
     9721 cctgaagcgc tatccgaaga tgcgcatctc cctcgcgctg ctcgaccggg tggtgaacct
     9781 cgtcgaggag ggtctcgacg tcggcgtgcg cattggccac ctgcaggact cgagcgtcgt
     9841 ggcgcgcccg gtcggccgca tccgccgcgt cgtggtcgca agccccgcgc ttctgcggcg
     9901 ggtcggtacg ccgaagcatc cgagcgaact cgcccgcctg ccctgcctgc acttcagcgc
     9961 caccgacggc agccgctggg ccttccggca cgcgggccgc accgtcacgg tcgatgtgca
    10021 gggatcgttc gagtgcaacc tggcggcgcc gatcatggac gcctgtgcgg ccggtctcgg
    10081 attcggacgc tgcctgtcgt accaggcggc gccgctgctg gcggaccggc acctgcgcat
    10141 cgtgctcgcc gactacgaga tcgagccgtg gcccgtccac ctcacctatc cgagcgcgcg
    10201 attgctgcct gcgcgctcca aggtgttcat cgaatggatg atcgaagcgc tgaaggcttc
    10261 gctgcagcgg acggtaccgg tgtgagcact tctcccaccc cggcccgccg gcagggcgcg
    10321 tgcggcgact ggaaacggcg gaaaggccat cgcatgtacc gcgaagtcgc gtatcgcgcg
    10381 aacgtcattg gcaaaatagg ctcgaacgac acgtccgccg cggccgcccc tggccgggga
    10441 cgcaccgcgg ccgagccaga cggagcgaca ccatgatcga actgaacgtc aacggcgcca
    10501 ggcaccgcgt cgacgtcgaa cccgagatgc cgctgctctg ggtcctgcgc gaccacctga
    10561 acctgcatgg caccaagtat ggctgcggaa tctccgtctg cggcgcgtgc acggtgcatg
    10621 tcgacgacgc ggccgtgcg
//
LOCUS       DBNHBY010000604         1647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_312960, whole genome shotgun sequence.
ACCESSION   DBNHBY010000604 DBNHBY010000000
VERSION     DBNHBY010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_312960"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1159)
                     /locus_tag="AC16HB_14650"
     CDS             complement(101..1159)
                     /locus_tag="AC16HB_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HIR5608074.1"
                     /translation="MSFKITVRPSGREFTVDDGETILAAALRGGVGLPYGCKNGACGT
                     CKAQGVEGEWAQGPHSSSALTPEEQARRKLLVCCSRPQTDVVIEARILTGFGDIPIRK
                     MPCRIASIEMAAPDVAIVSLQLPANERLQYRAGQYLEFILKDGSRRSYSIATAPHADE
                     KLTLHIRHMPGGVFTDALFGVRQPAVKERDILRIEAPLGTFFLREDNAGPIVLLASGT
                     GFAPIKAIAEHIFHKRLNRDEADRPARPVVFYWGCRARRDLYLPALPEQWAREQPNFT
                     YVPVLSDATPEDAWTGRTGFVHQAVMEDLPDLSAHQVYACGAPVMVDAARRDFTAKCR
                     LPEDQFFADAFTSQADLA"
     gene            1171..>1647
                     /locus_tag="AC16HB_14655"
     CDS             1171..>1647
                     /locus_tag="AC16HB_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR5608075.1"
                     /translation="MQKKSPAEPGSRGRCRFGAPRLLIVGCGDVGLRIVARLRTRYRI
                     VALTSAPARRAELRAAGALPIVGDLDVRGSLARIAAFGARVIHLAPPPNRGTRDPRTR
                     HLLAALRPVERCVYISTTGVYGDRAGACIDETAPIAPANERALRRGDAERRVRAAGA"
BASE COUNT          233 a          596 c          559 g          259 t
ORIGIN      
        1 ccggtgacgt gtcctacgcg cgcgctcccc tgcggagttc cgcatggcgt cggctgcgtc
       61 ggctgatcat gtgcgtcatc gcatgcacta tacctcagga ttaagccagg tcagcttgcg
      121 acgtgaacgc gtcggcgaag aactggtcct cgggcagcct gcacttcgcc gtgaagtcgc
      181 gccgtgcggc gtcgaccatc accggcgcgc cgcatgcgta gacctgatgc gcggagagat
      241 cgggtaggtc ttccatcacg gcctggtgga cgaacccggt gcggccagtc cacgcatcct
      301 ccggcgtcgc gtccgacagt accggtacgt aggtgaagtt gggctgctcg cgcgcccact
      361 gctccggcag cgcgggcagg tagagatcgc gccgcgcgcg gcagccccag tagaacacga
      421 ccggccgcgc cggccggtcc gcctcgtcgc gattcaggcg cttgtggaag atgtgctcgg
      481 cgatcgcctt gatcggcgcg aagcccgtgc cgctcgcgag cagcacgatc ggacccgcgt
      541 tgtcctcgcg caggaagaac gtaccgagcg gcgcctcgat gcgcaggatg tcgcgctcct
      601 tgaccgccgg ctgcctgacg ccgaacagcg cgtcggtgaa cacgccgccc ggcatgtgac
      661 gaatgtgcag cgtgagcttc tcgtcggcgt gcggcgcggt cgcgatcgag tagctgcggc
      721 ggctgccgtc cttcaggatg aattcgaggt actgaccggc gcggtactgc agtcgctcgt
      781 tggccggaag ctgcagcgaa acgatcgcca cgtccggcgc ggccatttca atgctcgcga
      841 tccggcacgg catcttgcgg atcgggatgt cgccaaagcc ggtcaggatg cgcgcttcga
      901 tcaccacgtc ggtctgcggc cggctgcagc acaccagcag cttgcgccgg gcctgctcct
      961 cgggcgtcag cgcggacgag gaatgcgggc cctgtgccca ctcgccttcc acgccctgtg
     1021 ccttgcaggt gccgcaggcg ccgttcttgc agccgtacgg cagccccacg ccgcccctca
     1081 gcgctgcggc gaggatcgtc tcgccgtcgt cgacggtgaa ctcgcggccc gacggccgca
     1141 ccgtgatctt gaagctcata gaatcgaacg atgcaaaaaa aatcgcccgc ggaacctggc
     1201 tcgcgcgggc gctgtcgctt cggggcgccg cgcctgctga tcgtcggctg cggcgacgtc
     1261 ggcctgcgga ttgtcgcacg gctgcgtacg cgctatcgca tcgtcgcact cacgtcagcg
     1321 cccgcgcggc gcgccgaact gcgcgcggcc ggcgcactgc cgatcgtcgg cgatctcgat
     1381 gtccgcggct cgctcgcgcg catcgccgcg ttcggcgcgc gcgtcatcca cctcgcgccg
     1441 ccgcccaatc gcggcacgcg cgatccgcgc acccgacacc tgctcgcggc gctgcgtccg
     1501 gtcgagcgct gcgtctacat cagcaccacc ggcgtgtacg gcgatcgcgc gggcgcatgt
     1561 atcgacgaga ctgcgcccat cgcaccggcc aacgagcgcg cgctccgccg cggcgacgcc
     1621 gagcgccgcg tgcgcgccgc cggcgcc
//
LOCUS       DBNHBY010000605         4021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1395544, whole genome shotgun sequence.
ACCESSION   DBNHBY010000605 DBNHBY010000000
VERSION     DBNHBY010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1395544"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..919
                     /locus_tag="AC16HB_14660"
     CDS             122..919
                     /locus_tag="AC16HB_14660"
                     /EC_number="2.7.7.-"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIR5608076.1"
                     /translation="MDPLAFRARLLAALLPPLQAAEFIAACFEGGSAAMGRLDAWSDI
                     DLYFVAGADDTAAVFDRFEATLASLARIAHTWRVDPPPFPGLAQRHYLLDPAPPHFEV
                     ECCVLTPDGVAQFLERERHGEPIVHFDRTGRVRGVPLDRAVHATRLARRFEQIRAAWP
                     IYRQIVAKEIERGHPLDAYGFYVNSLLRPLIELAGMRHRPERFDYGWRYLHHDLPADL
                     QRALTQLAYVASLDDLAARLPQLDCLMARLASEIEGHAPILTRAALN"
     gene            1119..1406
                     /locus_tag="AC16HB_14665"
     CDS             1119..1406
                     /locus_tag="AC16HB_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608077.1"
                     /translation="MLMRVSEHGDEVLVELTGVAGRQQRVLQALNECQRAAAGDRAAA
                     LASTQVSVRAGSNDMHIRLKAGNGIRFEATAIYRCLRHALFETAAVPLEAR"
     gene            complement(1431..2120)
                     /locus_tag="AC16HB_14670"
     CDS             complement(1431..2120)
                     /locus_tag="AC16HB_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608078.1"
                     /translation="MRRLAAVLLYLLLAPAYAGRPFTTEDAGVLAERECELEAYLLRA
                     SARNEPIARGGSIQPNCGIGWQTQFGLGAARIEAGGEATNSLALVGKTALRELTATQT
                     GIALAYSVVGVKPSGRGFRHDATAMGAVVSTPLATEWLLHANLGWLRTQVDRVDTTTW
                     AVALERTGVIEGFEVGAEVYGDDRTPGAFIGIGARYAVLPERFSVDLSWARRRSGARA
                     TLATLGIRIGF"
     gene            complement(2130..3134)
                     /locus_tag="AC16HB_14675"
     CDS             complement(2130..3134)
                     /locus_tag="AC16HB_14675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5608079.1"
                     /translation="MQRLRIATLNCLNLALPGRRFYPGVDPYSADEYIAKTQWLATMF
                     DRVGADLVLLQEIFHEQALSDVVRQTAAGARAWAQAVPFADQANTLPRLGLAWRSSLH
                     PQVDSIVDLPPGCAVVVPDKGEHARFSRPLLRARLGLPNVAGGVALTLFNVHLKSRRP
                     EYLESENCDDPRLEARAQLRSLVMRGAEAAALRWLVIDATRGARAPLVVAGDFNDDIR
                     AVTTQMVVDTSWKPDDRPLRDHMLFDALDVERRQAPGRGRDVAYTNLHGGEAERIDQV
                     LVSEDFVPQSKRAIGHVASAAILNDHLVERRQSRDGAAPDLTRIYSDHAAVMVDVVFD
                     "
     gene            complement(3188..3559)
                     /locus_tag="AC16HB_14680"
     CDS             complement(3188..3559)
                     /locus_tag="AC16HB_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608080.1"
                     /translation="MEAALGRIGQIALQVSDIDRAEHFYRETLGLKPLFRFGNLAFFD
                     CGDVRLMLDGGHEPTGKRERFCVYFRVEGIEPVVAALKSRQVYFEHEPHRVAKMPDHE
                     LWMAFLHDPDGNLLALMEEKR"
     gene            complement(3564..4010)
                     /locus_tag="AC16HB_14685"
     CDS             complement(3564..4010)
                     /locus_tag="AC16HB_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608081.1"
                     /translation="MKKERKPGPRSGSAPRDYGKPRGTRPPSRLRGEERPPRPARFRG
                     DDAGASRDRGDPYRHVQRPVRTREEGGRGFTVTLDPDVARVFRGDASVNRALRLVLQL
                     MQVVQGPRQHADRPRPPRPGYQGSAEARGFARKPRFEGDEQAEPEE"
BASE COUNT          605 a         1456 c         1335 g          625 t
ORIGIN      
        1 cccgtacaag tatccgcgcg ccgtcgagtt catcgacgcg ctgccgcgca cggagaccgg
       61 caaggtacag cgcttcaagc tgcgccagcg cattgccgcg accggctgag cggcgacagc
      121 aatggacccg ctcgccttcc gcgcgcggct gctcgccgcg ctgctgccgc cgctgcaggc
      181 cgcggaattc atcgcagcgt gcttcgaagg cggatccgcg gcgatgggcc ggctcgacgc
      241 gtggtccgac atcgacctgt acttcgtcgc tggcgcagac gacacggctg cggtgttcga
      301 ccggttcgag gcgacgctcg catcgctggc acgcatcgcg catacgtggc gggtcgaccc
      361 gccaccattc cccggactcg cgcagcgcca ctatctgctc gatccggcgc cgccgcactt
      421 cgaggtcgaa tgctgcgtgc tgacgccgga cggcgtcgcg cagttcctgg aacgcgagcg
      481 gcatggcgag ccgatcgtcc acttcgatcg caccggtcgc gtgcgcggcg ttccgctcga
      541 tcgcgccgtg cacgcgacgc gactggcgcg gcggttcgag cagatccgcg cggcatggcc
      601 gatctaccgg cagatcgtcg ccaaggagat cgagcgcggg cacccgctcg acgcgtacgg
      661 cttctacgtc aacagcctgc tgcgcccgct gatcgagctt gcgggcatgc gccaccggcc
      721 cgagcgcttc gactacggct ggcgctacct gcaccacgat ctgccggccg acctgcagcg
      781 agcactcacg cagctcgcct atgtggcgtc gctcgacgat ctcgccgcca ggttgccgca
      841 gctcgactgc ctgatggcgc ggcttgcgag cgagatcgag ggccatgcgc cgatcctgac
      901 gcgtgccgca ctgaactgag cgcgcgctgt cgcgcggttg cggatgcgcg tgacccgcgt
      961 atcgggcaat gcccgcctcc gagcgtcgac atccgttcgt cgactggcgc agtcaaagcg
     1021 gttcgcgtac ggtttttgca ggacaggcgc cgcgatcatc gacacagcgt caacggacga
     1081 gagcggtcga tcgttgtgat ccggcacgga ggcgaacgat gctgatgcga gtcagcgaac
     1141 acggtgacga agttcttgtc gagctgaccg gcgtcgccgg acggcagcag cgcgtgctgc
     1201 aggcactgaa cgaatgtcaa cgtgccgccg cgggcgatcg tgcggccgcg ctcgccagca
     1261 cccaggtgtc ggtccgcgcc gggtccaacg acatgcacat ccgcctgaag gccggcaacg
     1321 gcatccgctt cgaggcgacc gcgatctacc gctgcctgcg ccacgcgctg ttcgagacgg
     1381 ccgccgtacc gctcgaagcg cgctgactcg tccggcgccg cggcgccgct tcagaacccg
     1441 atcctgattc ccagcgtggc cagcgtggcg cgcgcacccg acctgcgccg cgcccaggaa
     1501 aggtcaacgg agaaccgctc cggcagcacg gcataacgtg cgccgatgcc gatgaatgcg
     1561 cccggcgtgc gatcgtcgcc gtagacctcg gcaccgacct cgaagccttc gatgactccg
     1621 gtacgctcga gcgcgaccgc ccacgtcgtc gtgtcgacgc gatcgacttg cgtccgcagc
     1681 cacccgaggt tcgcgtgcag aagccattcc gtggcgagcg gcgtcgacac gaccgccccc
     1741 atcgcagtgg cgtcgtgcct gaatccccgg ccggagggct tcacgccaac gacgctgtac
     1801 gcgagcgcga ttcccgtctg tgtcgcggtc agttcgcgca gcgcggtctt gccgaccagg
     1861 gcgagcgagt tcgtcgcctc gccacccgcc tcgatacgcg cggcaccgag gccgaactgc
     1921 gtctgccagc cgattccgca attgggctgg atggagccgc cgcgcgcgat cggttcgttg
     1981 cgcgcgctcg cgcgcagcag atacgcctcc agctcgcatt cgcgctcagc gagcacgccc
     2041 gcgtcctcgg tcgtgaacgg tcgcccggcg tacgccgggg ccaacagcag gtacagcagc
     2101 accgccgcga gcctgcgcat gggatccgct cagtcgaaca ccacatcgac cattacggct
     2161 gcgtgatcgg aatagattcg cgtgaggtcg ggtgcggcgc cgtcgcgcga ctgccgtcgc
     2221 tcgacgagat ggtcgttcag gatcgcagcc gaagcgacgt gtccgatcgc gcgcttcgat
     2281 tgcgggacga agtcctccga caccagcacc tgatcgatcc gctccgcctc gccgccgtgc
     2341 aggttcgtgt aggcaacgtc gcgcccgcgt cccggcgcct ggcgcctctc cacatcgagc
     2401 gcgtcgaaca gcatgtggtc gcgcagcggc cggtcgtccg gcttccatga ggtgtcgacg
     2461 accatctgcg tggtcaccgc gcggatgtcg tcattgaagt cgccggcgac gacgagcggc
     2521 gcacgcgcac cgcgcgtggc atcgattacg agccaccgca gcgccgccgc ctccgcgccg
     2581 cgcatcacga gcgatcgcaa ctgcgcgcgc gcttccagcc gcggatcgtc gcagttctcg
     2641 ctttccaggt attccggccg gcgcgatttc aggtgcacat tgaacagcgt cagcgcaacg
     2701 ccgccggcaa cgttcggcag gccgagccgg gcgcgcagca gcgggcgcga aaagcgcgcg
     2761 tgctcgccct tgtccgggac gacgaccgcg cagccgggcg gcaggtcgac gatcgagtcg
     2821 acctgcgggt gcagcgaact gcgccaggcg agccccagcc gcggcaaggt gttggcctga
     2881 tccgcgaacg gcaccgcctg cgcccacgcc cgcgcgcccg cggcggtctg gcgcaccaca
     2941 tccgacagcg cctgctcgtg gaaaatctcc tgcagcagca cgagatcggc accgacgcga
     3001 tcgaacatcg tcgccagcca ctgcgtcttg gcgatgtatt cgtcggcact gtacgggtcg
     3061 accccgggat agaagcggcg ccccggcagc gccaggttca ggcaattgag cgtggcgatg
     3121 cgcaggcgct gcatcgcgcg acgttacaaa cggtgcgccc cgcggtcaat ggcgatcggt
     3181 tcgcgcctca gcgcttctct tccatcagcg ccagcagatt gccgtccggg tcgtgcagga
     3241 acgccatcca cagttcgtgg tcgggcatct tcgcgacccg gtgcggctcg tgctcgaagt
     3301 acacctgacg cgacttcaac gccgcgacca cgggttcgat gccctcgacg cggaagtaga
     3361 cgcagaaccg ctcgcgcttt ccggtcggtt cgtgtccgcc gtcgagcatc aggcgcacgt
     3421 cgccgcagtc gaagaacgca aggttcccga agcggaacag cggcttcagc cccaacgttt
     3481 cgcggtagaa atgctccgcc cgatcgatgt cggatacctg cagcgcgatc tgcccgatgc
     3541 gtccgagcgc tgcttccatc ggttcactcc tcgggttctg cctgctcgtc gccctcgaag
     3601 cgcggcttgc gcgcgaatcc gcgcgcctcg gcgctgccct ggtagccggg gcgcggcggg
     3661 cgtggacggt cggcgtgttg ccgtggtccc tgcaccacct gcatcaactg gaggaccagc
     3721 cgcaacgccc tgttcaccga tgcgtcgccc cggaacacgc gtgccacatc gggatcgagg
     3781 gtgacggtga agccgcgtcc gccctcctcg cgcgtccgaa ccggacgctg cacatgccgg
     3841 tatggatcgc cgcgatcccg gctcgcgccc gcgtcgtcgc cgcggaaccg cgccgggcgc
     3901 ggcggccgct cttcgccgcg caagcgcgac ggcggtcggg tcccgcgcgg cttgccgtag
     3961 tcgcgcggcg cgctaccgct gcgcggtccc ggcttgcgtt ccttcttcat cgctgtctcc
     4021 c
//
LOCUS       DBNHBY010000606         2769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_313135, whole genome shotgun sequence.
ACCESSION   DBNHBY010000606 DBNHBY010000000
VERSION     DBNHBY010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_313135"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="AC16HB_14690"
     CDS             <1..531
                     /locus_tag="AC16HB_14690"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608082.1"
                     /translation="SPIDGVVSRRLMELGELATPGKPVVDVFDPSRLRAVGAVPQFVL
                     ARVAQADHASVLLPTLNQTIAAAKLTLLPAADPRLLSTQVRADLPAQLPAGAVPGTAA
                     KILLPTGSASKLVMPADAVIRRGELTVTYVLAADGKPQLRQIRVGDSVEGQQVEVLAG
                     LAAGERVQLDPLLATR"
     gene            528..>2769
                     /locus_tag="AC16HB_14695"
     CDS             528..>2769
                     /locus_tag="AC16HB_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608083.1"
                     /translation="MSAPDSDAKLGVSGRIAHAFLTSQLTPLLALLALLLGIFAVLVT
                     PKEEEPQINVTMANVLIPFPGASSTDVHNLVTVPAEQVISQITGIEHVFSVTRPGMAI
                     LTVQFKVGEDRIAALVRLHDTLRANSDWLPRHLGVADPIVKPKGIDDVPIVTLTLAST
                     RADASAESLQRIAHAMEIELKRVSGTREVATLGGPDQVVRVTIDPERMAAHRVTVTDI
                     ERALRATNASAPSGSLVSGNRTVDVRTGEYFESARDVAQLVVGVFERRPVYLSDVARV
                     TAGADTPTRMVWHGEKVDGSYVELPAVTIWVSKKAGENAATVADAVLARVAELKGTVV
                     PSDTQVAVTRNYGDTARDKANKLIQKLVFATLSVVALVWATLGRREAAIVGVAVVLTL
                     AATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRRSAFDHAPPTEMIPRA
                     VDEVGGPTILATLTVIAALLPMAFVTGLMGPYMAPIPINSSMGMVLSLAIAFVITPWL
                     ALRLLHRHLGGHAAPASADKLSQRLAAFFARVMTPFLRGGDGKGNRRKLWIGIGIAIL
                     LSVALAPLKWVVLKMLPFDNKSEFQVVVDMPTGTAVERTAGVLRELAAEVAKVPEVTS
                     IQAYAGLAAPINFNGLVRQYYLRSGGEVGDLQVNLVDKHQRDRKSHDIAQAVLPALQA
                     IAKRSGADLKVVEVPPGPPVLSPIVAEIYGPDYDAQTKLAKQVRAVFEATPDIVAIDD
                     TTLDAAP"
BASE COUNT          421 a          919 c         1008 g          421 t
ORIGIN      
        1 tcgccgatcg acggcgtggt gtcgcgacgg ctgatggaac tgggcgagct cgccacgccc
       61 ggcaagccgg tggtcgacgt gttcgatccg tcgcggctcc gcgcggtcgg agccgtgccg
      121 cagttcgtgc tcgcgcgcgt ggcgcaggcg gatcacgcca gcgtgctgct gccgacgctg
      181 aaccagacca ttgccgcggc aaagctgacg ctgctgccgg cagccgatcc gcgcttgctg
      241 tcgacgcagg tgcgcgccga cctgccggcg cagttgccgg ccggcgcggt gcccggcacc
      301 gcggccaaga tcctgctgcc gaccggcagc gcgagcaagc tcgtgatgcc ggccgatgcg
      361 gtgatccgta gaggcgaact gacggtgacc tacgtgcttg ccgcggacgg caagccacag
      421 ctgcggcaga ttcgcgtggg cgactcggtc gaggggcagc aggtcgaagt gctcgcgggc
      481 ctcgcggccg gcgagcgggt gcaactggat ccgctgctgg ctacgcgatg agcgcccctg
      541 attccgacgc gaaactcggt gtctcaggac gcatcgcgca cgcgttcctc acctcacagc
      601 tgacgccgct gctggcgctg ctggcgctgc tgctcggcat cttcgcggtg ctggtcacgc
      661 cgaaggagga agagccgcag atcaacgtca cgatggccaa cgtgctgatc ccgttccccg
      721 gcgcgagcag caccgacgtg cacaacctcg ttaccgtgcc ggccgagcag gtgatctccc
      781 agatcaccgg catcgagcac gtgttctcgg tgacgcggcc gggcatggcg atcctgaccg
      841 tgcagttcaa ggtcggcgag gaccgcatcg ccgcgctggt gcggctgcac gacacgctgc
      901 gcgcgaactc cgactggctg ccgcggcacc tcggcgtggc cgatcccatc gtcaagccca
      961 agggcatcga cgacgtgccg atcgtcacgc tgacgctggc gagcacgcgc gccgacgcga
     1021 gcgccgagag cctgcagagg atcgcgcacg cgatggagat cgaactgaag cgggtgtcgg
     1081 gcacgcgcga agtggccacg ctcggcgggc cggatcaggt cgtgcgcgtg acgatcgacc
     1141 ccgagcggat ggccgcgcac cgtgtgacag tgaccgacat cgagcgcgcg ttgcgcgcga
     1201 ccaatgcgtc ggcgccgtcg ggctcgctgg tgagcggcaa ccgcacggtc gacgtgcgca
     1261 ccggcgagta cttcgagtcg gcgcgcgacg tggcgcaact ggtggtcggg gtgttcgaga
     1321 gaaggccggt gtacctgtcc gatgtcgcgc gcgtgaccgc gggcgccgat acaccgacgc
     1381 ggatggtctg gcacggcgag aaggtcgacg gcagctacgt cgagttgccc gcggtgacga
     1441 tctgggtgtc gaagaaggcg ggcgagaacg ctgccaccgt cgccgatgcg gtgctggcgc
     1501 gcgtggccga actgaagggc acggtcgtgc cgagcgacac gcaggtcgcg gtcacgcgga
     1561 actacggcga caccgcgcgc gacaaggcca acaaactgat ccagaagctc gtcttcgcca
     1621 cgctgtcggt agtcgcgctg gtgtgggcca cgctcggccg gcgcgaggcg gcgatcgtcg
     1681 gcgtcgcggt ggtgctgacg ctggcggcga cgctgttcgc ctcgtgggca tggggcttca
     1741 cgctgaaccg cgtgtcgctg ttcgcgctga tcttctcgat cgggattctc gtcgacgatg
     1801 cgatcgtcgt tgtagagaat atccaccgcc ggtccgcgtt cgatcacgca ccgccgacgg
     1861 agatgatccc gcgcgcggtc gacgaggtcg gcggtccgac gatcctggcc acactgaccg
     1921 tcatcgcggc gctgctgccg atggcgttcg tcaccgggtt gatgggcccg tacatggcgc
     1981 cgatcccgat caactcatcg atgggcatgg tgctgtcgct ggcgatcgcg ttcgtgatca
     2041 cgccgtggct ggcgctgcgg ctgctgcacc ggcatctggg cgggcacgcc gcgccggctt
     2101 cggccgacaa gctgtcgcag cggctggcgg cgttcttcgc ccgcgtgatg acgccgtttc
     2161 tgcgcggcgg agatggcaag ggcaatcgcc gcaagctgtg gatcggcatc ggtatcgcga
     2221 tcctgctgtc tgtggcgctg gcgccgctga agtgggtggt gctgaagatg ctgccgttcg
     2281 acaacaagag cgagttccag gtcgtggtcg acatgccgac cggcaccgcg gtcgagcgca
     2341 ccgccggcgt gctgcgcgag ctcgcggccg aggttgccaa ggtgcctgag gtgaccagca
     2401 tccaggccta cgcggggctg gccgcgccga tcaacttcaa cgggctggtg cggcagtact
     2461 acctgcgctc cggcggcgag gtcggcgacc tgcaggtgaa cctggtcgac aagcaccagc
     2521 gcgaccgcaa gagccacgac atcgcgcagg ccgtgctgcc ggcgctgcag gccatcgcga
     2581 agcgcagcgg cgccgacctc aaggtggtcg aggtgccgcc cggcccgccg gtgctgagcc
     2641 cgatcgtcgc ggagatctac gggcccgact acgacgcgca gacgaagctc gccaagcagg
     2701 tgcgcgcggt gttcgaggcc acgcccgaca tcgtcgcgat cgacgacacc acgctcgacg
     2761 ccgcaccgc
//
LOCUS       DBNHBY010000607         3657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_583232, whole genome shotgun sequence.
ACCESSION   DBNHBY010000607 DBNHBY010000000
VERSION     DBNHBY010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_583232"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16HB_14700"
     CDS             complement(<1..461)
                     /locus_tag="AC16HB_14700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5608084.1"
                     /translation="MLKIWGRLSSINVQKAVWAANEAGVSFERTDAGAAFGLVDTPWY
                     GALNPNRLVPVIDDDGFVLWESNVIVRYLAAKYAPGRLYPDDLRNRFDAERWMDWQQT
                     ALNPAGRDAFLQLIRTPEPQRNAALIEQSCGRTAPLLQMLDAHLSTRRFLTG"
     gene            complement(455..1660)
                     /locus_tag="AC16HB_14705"
     CDS             complement(455..1660)
                     /locus_tag="AC16HB_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR5608085.1"
                     /translation="MNAPVTELTAPLRPARRTEQVKSSMIREILKVTQRPDMISLAGG
                     LPAPETFPVDAMRAAFDAVLAHDGPGSLQYSTTEGYTALREWIAAQETARGAPTHADE
                     VLIVAGSQQALDLVGKAFAETGAPLLVEAPSYLGALQAFSVFEPVFRAIPTDAGGLIP
                     QAIDAARARDARIAYVMSNFSNPTGRTLSVERRAALAQAARATGLWLVEDDPYGELWY
                     RAAPPPSLRTWAPERTIRICSFSKVLAPGLRLGYVVAPRACIDLLVRLKQATDLHTAT
                     LSQRAAYRIVSSSDFPQHLARVRARYADQCRVMLAALEQSMPAGVEWTRPDGGMFVWL
                     TLPAHVDATALLPRAIERNVVYVPGEPFYALAPARNTLRLSFVTVPPAAIERGVRTLA
                     ELVAEQLRC"
     gene            complement(1688..2281)
                     /locus_tag="AC16HB_14710"
     CDS             complement(1688..2281)
                     /locus_tag="AC16HB_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5608086.1"
                     /translation="MSGEQLIALVVFALAGSFTPGPNNTIATATGANHGLRATLPHIL
                     GVPFGLSTMLVAGALGAAALIAAHPALATAIRWTGIAYLLYIAWSIARTAAIGDRPLA
                     RPLTFWQSAAFQYANPKAWMLAAATAGTYMAADEALARVVVICAVFSAACAASLVVWA
                     ATGAALKDWLKCGTRLRAFNAVMGALLAATALWMAFE"
     gene            complement(2281..3249)
                     /locus_tag="AC16HB_14715"
     CDS             complement(2281..3249)
                     /locus_tag="AC16HB_14715"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HIR5608087.1"
                     /translation="MSLPARASLLSAAEIVHRTLTPTPQFAWPLLAEALGAEVWVKHE
                     NHLPVGAFKLRGGLVYFDELKQREPACAGVVTATRGNHGQSVGFAARTAHLPATIVVP
                     HGNSREKNAAMRALGVELIEHGHDFQAAREEAARVAAARRLHFIPAFHRDLVRGVASY
                     WLEVFDAVPDLSVVFVPIGQGSGINACIAAREALGLNTRIVGVVSAQAPCYALSYRAG
                     RAIEAPVATKLADGLACRVPDADSLDVIRRHVDDVIEVDDAEVAGAMRLLFAATHNVA
                     EGAGAASLAAAYKSRQRIAGKRVGVVITGGNVDSDQFARVLAGGDH"
     gene            3372..>3657
                     /locus_tag="AC16HB_14720"
     CDS             3372..>3657
                     /locus_tag="AC16HB_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5608088.1"
                     /translation="MNLDERSDSHPDADFPSVLGADDRTLAAQLAARLALRIDEHALK
                     PGTRLPSIRAYAAQQHVSRSTVVDAYDRLIAAGLIDSRRGSGFFVAARPRR"
BASE COUNT          571 a         1275 c         1285 g          526 t
ORIGIN      
        1 ccggtcagga agcggcgcgt ggacagatgc gcatccagca tctgcagcag cggagcggtc
       61 cttccgcagg actgctcgat cagcgccgcg ttgcgctgcg gctccggcgt gcggatcaat
      121 tgcaggaacg catcgcgccc ggccgggttc agcgcggtct gctgccagtc catccagcgc
      181 tcggcgtcga agcggttgcg cagatcgtcc gggtacaagc gtcccggcgc gtatttcgcc
      241 gcgaggtagc gcacgatcac gttcgactcc cacagcacga agccgtcgtc gtcgatcacc
      301 ggcaccagcc ggttcgggtt cagcgcgccg taccacggcg tgtcgaccag accgaacgca
      361 gcacccgcat cggtgcgttc gaagctcaca cccgcttcgt tcgcggccca gaccgccttc
      421 tgcacattga tcgaactcag gcggccccag atcttcagca tcgaagttgc tccgcgacga
      481 gttccgccag cgtccgcacg ccgcgctcga tcgcagccgg cggcacggtg acgaacgaaa
      541 gccgcagcgt gttgcgcgcg ggtgcaagcg catagaaagg ctcgcccggc acgtaaacca
      601 cgttgcgctc gatcgcgcgc ggcagcagcg cggttgcatc gacgtgcgcc ggcagcgtca
      661 gccagacgaa catgccgccg tcggggcgcg tccattcgac gccggccggc atcgattgct
      721 cgagcgcggc cagcatcacg cggcactggt cggcgtaacg cgcgcgcaca cgcgcgagat
      781 gctgcgggaa atcgctgctc gagacgatcc ggtacgcggc gcgctgcgac agcgtggcgg
      841 tgtgcaggtc ggtcgcctgc ttcagtcgca ccagcagatc gatgcaggcg cgcggcgcga
      901 cgacatagcc gagccgcagc cccggcgcga gcaccttgga gaacgagcag atgcgtatcg
      961 tgcgctcggg cgcccacgtg cgcagactcg gcgggggtgc cgcgcggtac cacagttcgc
     1021 cgtacggatc gtcctcgacc agccacaagc cggtcgcgcg cgccgcttgc gccagtgccg
     1081 cgcggcgctc gacgctcagg gtgcgaccgg tcgggttgga gaagttcgac atcacgtagg
     1141 cgatgcgggc gtcgcgcgcg cgtgccgcgt cgatcgcctg cgggatcagt ccgccggcat
     1201 cggtcgggat cgcgcggaac accggctcga acaccgagaa cgcctgcagc gcgccgagat
     1261 agctcggcgc ctcgaccagc agcggcgcgc ccgtctccgc gaatgccttg ccgacgaggt
     1321 cgagcgcctg ctggctgccg gcgacgatca gcacctcgtc ggcgtgcgtc ggcgcgccgc
     1381 gtgcggtctc ctgcgcggcg atccattcgc gcagcgcagt gtagccctcg gtcgtgctgt
     1441 attgcagtga gccggggccg tcgtgcgcga gcaccgcgtc gaatgccgcg cgcatcgcgt
     1501 cgaccggaaa ggtctccggc gccggcaggc cgcccgccag cgaaatcatg tcgggccgct
     1561 gcgtgacctt caggatctcg cggatcatcg aactcttgac ctgctcggtg cggcgcgcgg
     1621 gccgcagcgg tgcggtgagt tcggtgacgg gtgcgttcat ctcatttctc ctcgggagtc
     1681 gaagcgttca ctcgaaggcc atccatagcg ccgtcgccgc gagcagcgcg cccatgacgg
     1741 cgttgaaagc gcgcaagcgc gtgccgcact tcagccagtc cttcagcgcc gcgccggtgg
     1801 cggcccagac gacgaggctc gccgcgcacg cggccgagaa caccgcacag atcacgacga
     1861 cacgcgccag cgcctcgtcg gctgccatgt aggtgccggc ggtggcggcg gcgagcatcc
     1921 acgccttggg gtttgcgtac tggaacgccg ccgactgcca gaaggtcaga ggtcgcgcga
     1981 gcgggcggtc gccgattgcc gccgtgcggg cgatcgacca cgcgatgtac agcaggtaag
     2041 cgatgccggt ccagcggatc gcggtcgcga gtgcggggtg ggcggcgatc agcgcggcgg
     2101 cgccgagcgc cccggcgacc agcatcgtcg acaggccgaa gggcacgccg aggatgtgcg
     2161 gcagcgtggc gcgcagcccg tggttggcgc cggttgcggt cgcgatcgtg ttgttcggcc
     2221 ccggcgtgaa cgagcccgcg agtgcgaaca cgaccagagc gatcaactgc tcgccgctca
     2281 tcagtggtcg ccgcccgcga gcactcgcgc gaactggtcg gaatcgacat tgccgccggt
     2341 gatcacgacg cccacgcgct tgcctgcgat ccgctggcgc gacttgtatg cggcggcaag
     2401 cgatgcggcg ccggcgccct cggcgacgtt gtgggtggcg gcgaacagca gccgcatcgc
     2461 gcctgcgacc tcggcgtcgt cgacctcgat cacgtcgtcg acatggcggc ggatcacgtc
     2521 gagcgaatcc gcatccggaa cacgacacgc cagcccgtcg gccagcttcg tagccaccgg
     2581 cgcttcgatt gctcggccag cgcggtacga cagcgcgtag cacggcgcct gcgccgacac
     2641 cacgccgacg atgcgcgtgt tcagccccag cgcttcgcgc gcggcgatgc aggcattgat
     2701 gcccgagccc tgaccgatcg gaacgaaaac cacagagagg tccggtaccg cgtcgaacac
     2761 ctccaaccag tagctcgcca ccccgcgcac gaggtcgcgg tgaaacgcgg gaatgaaatg
     2821 cagccgacgc gccgcggcga ctcgagcggc ttcttcgcgt gcggcctgga aatcgtgacc
     2881 gtgctcgatc agctcgacgc cgagtgcgcg cattgcggcg ttcttctcgc gcgagttgcc
     2941 gtgcggaacg acgatcgtcg ccggcaggtg cgcggtgcgc gccgcgaagc cgaccgactg
     3001 gccgtggttc cctcgggtcg cggtgacgac gccggcgcag gcgggctcgc gctgcttcaa
     3061 ctcgtcgaag tacaccaggc cgccacgcag cttgaacgcg ccgaccggca ggtgattctc
     3121 gtgcttgacc cagacctccg cgccaagcgc ttcggcgagc agcggccatg cgaactgcgg
     3181 cgtcggcgtg agcgtgcggt ggacgatctc ggcggcgctc aggagcgacg cgcgagcggg
     3241 cagggacatg gcgcacccgg gtggcggcaa gaacgaggct tgcaatctag cagcgtcgac
     3301 cggtacagtg ccagtacagt ttcgcgtcca attcgaccca ctggcccgct cgattcggca
     3361 gcacagttgc gatgaacctc gacgaacgca gcgacagtca ccccgacgcg gatttcccgt
     3421 ccgtgctcgg cgcggacgac cgcacgctcg cggcgcagct cgcggcgcgg ctggcgctgc
     3481 gcatcgacga gcacgcactg aagccaggca cgcggctgcc ctcgatccgc gcctacgcgg
     3541 cgcagcagca cgtcagccgc tcgacggtcg tcgacgcgta tgaccggctg atcgcggccg
     3601 ggctgatcga ttcgcggcgc ggttcgggct ttttcgtcgc ggcgcggccg cggcgtg
//
LOCUS       DBNHBY010000608         1718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_853098, whole genome shotgun sequence.
ACCESSION   DBNHBY010000608 DBNHBY010000000
VERSION     DBNHBY010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_853098"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16HB_14725"
     CDS             <1..202
                     /locus_tag="AC16HB_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5608089.1"
                     /translation="LDRVLGKTPLSYFQDLRIEHAVHRLQTGTASVDAIANEVGYADG
                     VTLRTLLRRKLGRGVRELRGRG"
     gene            complement(320..1483)
                     /locus_tag="AC16HB_14730"
     CDS             complement(320..1483)
                     /locus_tag="AC16HB_14730"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR5608090.1"
                     /translation="MKLVAKGKVRTLLAVAAVVSVGACGSGDDESVLKSAKVGVSLTD
                     AAACGFDHVYVTVEKVRIHASSTADERSAGWTDIVVNQPKKIDLLGLTNGQVQELGQV
                     PIADGDYTQLRLVLRLNGNSVVPTGGLETALQTPSDVQNGIKVIRPFSVASNKRVDVV
                     LDFDACRSIVQQGSSNYSLKPVVTGQLIDAAIEGVVDPTVTDAIVSVQKNGEVIRSTV
                     PVAGTGAFSIPFLDSVQSPYEVVVTAAGRSTAVITGVTATKSTVTSLGTIAMPVSGLL
                     TPSRTASGTVDPVAARDAAEVRAMQAVGVPAVEIGFRNVNSVTGAYTLSLPLEAPRLA
                     LYSTTLPLTFTAHTATEGRYTLQARATDFTPKIEPADIRTTNLTINFTLDVAP"
BASE COUNT          289 a          576 c          545 g          308 t
ORIGIN      
        1 cctggaccgc gtgctgggca agacgccgtt gtcgtacttc caggatttgc gcatcgagca
       61 cgccgtgcat cggctgcaga ccggcactgc gagcgtggac gcaatcgcca acgaagtggg
      121 ttatgccgac ggcgtcacct tacggacgct cttgcggcgc aagctggggc gcggcgtgcg
      181 cgagctgcgc ggacgcggct agccgtgtcg tacgaccgcg cgcagacgcc gggcttgcgc
      241 cttcgcgcag cgttggtccg tcggttcccg cgagtcggtg atcccgccgc ggtgagttca
      301 gcgtcagcgc gccgacaacc tacggcgcaa cgtccagcgt gaagttgatc gtcaggttcg
      361 tcgtgcgaat gtcagccggc tcgattttcg gagtgaagtc tgtcgcgcgc gcctgcagcg
      421 tgtaccgacc ctcggtcgcc gtgtgcgcag tgaaagtcag cggcagagtc gtcgaataca
      481 acgcaagtcg cggcgcttcg agcggcagtg acagcgtgta ggcaccggtc accgagttga
      541 cgttgcggaa gccgatctcg accgccggca caccgaccgc ctgcatcgct cgcacttcgg
      601 cagcgtcgcg cgccgcgacc gggtcgacgg tgccgctggc cgtgcgcgat ggtgtcagca
      661 ggccggagac cggcatcgcg atcgtgccga gcgatgtgac agtcgacttt gtcgcggtca
      721 cgccggtgat cacggccgtc gagcggcccg ccgcggtaac gacgacctcg tacggcgatt
      781 gcaccgaatc caggaaggga atgctgaagg cgcccgtgcc tgccaccggc accgtgctgc
      841 ggatcacctc gccgttcttt tgcaccgaaa cgatggcgtc ggtgacggtc gggtccacca
      901 cgccttcaat cgccgcgtcg atcagctggc cggtgacgac cggcttcagc gaatagttgc
      961 tgctgccctg ctggacgatc gatcggcaag cgtcgaagtc cagcaccacg tcgacccgtt
     1021 tgttggaggc cacactgaac gggcggataa ccttgatgcc attctgcacg tcgctcggcg
     1081 tctgaagcgc ggtttcgagg ccgcccgtcg gtacgaccga attgccattc agcctgagca
     1141 ccagccgtag ttgcgtgtag tcgccgtccg cgatcggcac ctggccaagc tcctgcacct
     1201 ggccgttggt caacccgagc agatcgatct tcttcggctg gttgacgacg atgtcggtcc
     1261 agcccgcgct acgttcatcg gcggtcgacg aggcgtgaat ccgcaccttc tcgactgtca
     1321 cgtacacgtg gtcgaagcca cacgccgccg catcggtcag cgacacgccc actttggccg
     1381 acttcaagac ggattcatcg tcaccgctgc cgcacgcgcc aacggagacg acggctgcga
     1441 cagccagcag cgtccgcacc ttccctttcg caacgagttt cattgcccgc cctcatgcaa
     1501 tgtcgtcgga cgaccgtcga caggtcgacc ccggcacgac cacccaagcc cttcaatcat
     1561 cgcgctaggt taacttgatt gccggagaac tcgcagacgc aacaacgccc cggcatgatt
     1621 gttcacgcct gttaccttgc cgttctcctc atgaggccgg tgcagtcggc gcccgcgggc
     1681 gagccgcact ggatcatcga ctcgcgggac tggctgaa
//
LOCUS       DBNHBY010000609         2492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1328147, whole genome shotgun sequence.
ACCESSION   DBNHBY010000609 DBNHBY010000000
VERSION     DBNHBY010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1328147"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16HB_14735"
     CDS             complement(<1..279)
                     /locus_tag="AC16HB_14735"
                     /inference="COORDINATES: protein motif:HMM:NF042524.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HIR5608091.1"
                     /translation="MSLTKTVQRRESSWLLLTYKVPPEPSKLRVAIWRRIRGLGAVYL
                     QNGIRVLPTTTGHERQFRIVQSEIVRAGGESMLEAYASEVFEREHSAKG"
     gene            complement(276..1970)
                     /gene="ilvD"
                     /locus_tag="AC16HB_14740"
     CDS             complement(276..1970)
                     /gene="ilvD"
                     /locus_tag="AC16HB_14740"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5608092.1"
                     /translation="MTSNRRSKNIMEGVARAPNRSMYYALGYTAADFRKPMIGVANAH
                     STITPCNSGLQKLADAAVAGIEEAGGKAQVFGTPTVSDGMAMGTEGMKYSLVSREVIA
                     DCIETCVQAQWMDGVVAIGGCDKNMPGAVMGMLRANVPGIFVYGGTILPGHYQGRDLN
                     IVSVFEAVGEFTAGRMSEEDFCQIERRAIPGTGSCGGMYTANTMSSAIEALGLSLPYS
                     STMANPHEEKLTSAKASARALVEALKHDLKPRDIVTREAIENAVAVVMATGGSTNAVL
                     HLLAIAHAAEVEWKIDDFERVRRRVPVLCDMKPSGKYLAVDLHRAGGIPQVMKVLLNA
                     GLLHGDCMTITGKTVAQNLADVPAVPRADQDVIHAIDTPLYSHGHLAILKGNLAPEGC
                     VAKITGLKNPAMTGPARVFDDEQSALAAIMAQRIRPGDVMVLRYLGPRGAPGMPEMLA
                     PTSALIGQGLGESVGLVTDGRFSGGTWGMVVGHVAPEAYAGGNLALVQEGDSISIDAH
                     TLTLQLNVSDAELARRRQAWQAPPPRYTRGVMAKFAMNASSASTGAVLDQFGPPAR"
     gene            complement(2254..>2492)
                     /locus_tag="AC16HB_14745"
     CDS             complement(2254..>2492)
                     /locus_tag="AC16HB_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608093.1"
                     /translation="LQAATRPGGTLIVNVLTRGTTFMDMFDPDAHCLFEPERLDALFA
                     GWNILRCDDSRFPAPHETVKAFRTLVASRIGDAH"
BASE COUNT          388 a          853 c          814 g          437 t
ORIGIN      
        1 gcccttcgca ctgtgctcgc gctcgaagac ttcgctggca tacgcctcga gcatcgactc
       61 gccgccagcg cgcacgatct ccgactgaac gatgcggaac tggcgctcgt gacccgtcgt
      121 cgtcggcagc acgcggatgc cgttctgcag gtacaccgcg cccagaccgc ggatacgccg
      181 ccagatcgcg acccgcagct tggaaggttc gggcgggacc ttgtaggtca acagcagcca
      241 ggaggattcc cgccgctgca cggttttggt cagactcacc tggccggcgg cccgaactgg
      301 tcgagcaccg cccccgtgct tgcactggag gcgttcatcg cgaactttgc catcacgccg
      361 cgcgtatagc gcggcggcgg ggcttgccac gcctggcggc gccgggcgag ttccgcatcg
      421 ctcacgttca gctgcagcgt cagcgtgtgc gcgtcgatgc tgatcgagtc gccttcctgt
      481 acgagcgcga ggttgccgcc ggcgtaagcc tcgggagcaa cgtggccgac caccatgccc
      541 caggtcccgc ccgaaaagcg gccgtcggtg acgaggccga ccgattcgcc gaggccctgg
      601 ccgatcaatg cactcgtggg agccagcatt tcgggcatgc ccggcgcgcc gcgcgggccg
      661 aggtagcgca gcaccatcac gtcgcctggc ctgattcgct gcgccatgat cgccgccagc
      721 gccgactgct cgtcgtcgaa cacgcgggcc ggccccgtca tcgcggggtt cttcagcccg
      781 gtgatctttg cgacgcaccc ttcgggcgcg aggttgccct tgagaatcgc gagatgaccg
      841 tgcgagtaga ggggggtgtc gatggcgtgg atcacgtcct ggtcagcacg cgggacagcg
      901 ggcacgtcgg cgaggttctg cgcgacggtc ttgccggtga tggtcatgca gtcgccatgc
      961 agcagtcccg cgttgagcag caccttcatc acctgcggaa tgcccccggc acggtgcagg
     1021 tcgacggcta aatacttgcc cgacggtttc atgtcgcaca gcaccggcac gcgccggcgc
     1081 acgcgctcga agtcatcgat cttccattcg acttccgccg cgtgcgcgat ggcgagcaga
     1141 tgcagtaccg cgttggtcga gccgccggtc gccatcacga cggccaccgc gttttcgatc
     1201 gcctcgcggg tcacgatgtc gcgcggcttc aggtcgtgct tcagggcctc gaccagcgcg
     1261 cgggcggatg ccttcgccga ggtgagcttc tcctcgtgcg gattggccat cgtcgacgaa
     1321 tacggcaggc tcaggcccaa cgcttctatg gcggaactca tcgtgttggc ggtgtacatg
     1381 ccgccgcacg agccggtgcc tggaatggcg cggcgttcga tctggcagaa gtcctcttcg
     1441 ctcatgcggc cggccgtgaa ttcgcccacg gcctcgaaga cgctgacgat gttcagatcc
     1501 ctgccctggt agtgcccggg caggatagtg ccgccgtaga cgaagatccc cggcacgttg
     1561 gcgcgcagca tgcccatcac tgctccgggc atgttcttgt cgcagccgcc gatggcgacg
     1621 acgccatcca tccactgcgc ctgcacgcag gtctcgatgc agtcggcgat cacctcgcgc
     1681 gacaccagcg agtacttcat gccctcggtg cccatcgcca tgccgtcgga gacggtcggc
     1741 gtgccgaaga cctgcgcctt gccgcccgcc tcctcgatgc cggcgaccgc cgcatcggcc
     1801 agcttctgca ggccggagtt gcagggcgtg atcgtcgagt gcgcgttcgc cacgccgatc
     1861 atcggcttcc tgaagtcggc tgcggtgtag cccaacgcgt agtacatcga gcgattgggc
     1921 gcgcgcgcca cgccttccat gatgttcttc gatcggcgat tgctggtcat gtccacctcc
     1981 gcgggctggg gttcgaatca tatccatcgt cgccgcgccc attgcggacg cgagtccaag
     2041 ttgccaccgg gagtttcggc cgcccgggcg ggacgccatg ggtcggcaag cacggaaaca
     2101 gacgaccccg ttcagcttca atgaaacctc ggcgtcgtca gcctgttcgc cgacatgacc
     2161 tatgaaggcg cgcgcacgct ccgggcgtgg ccggcgagcc gctcgacacg ggctgtcgca
     2221 gaaatctgtt cgctgaccgc gccgacggtc ttttcaatgc gcgtcgccaa tccgcgatgc
     2281 caccagggtg cgaaatgcct tgaccgtctc gtgcggcgcg ggaaagcgcg aatcgtcgca
     2341 gcggaggatg ttccagcccg cgaacagcgc gtcgagccgc tccggttcga acaggcagtg
     2401 cgcgtcggga tcgaacatgt ccatgaaggt cgtgccacgt gtcaaaacgt tgacgatcag
     2461 cgtgccgccc ggccgcgtcg ccgcctgcag cc
//
LOCUS       DBNHBY010000610         5068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1801190, whole genome shotgun sequence.
ACCESSION   DBNHBY010000610 DBNHBY010000000
VERSION     DBNHBY010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1801190"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2387)
                     /locus_tag="AC16HB_14750"
     CDS             complement(<1..2387)
                     /locus_tag="AC16HB_14750"
                     /inference="COORDINATES: protein motif:HMM:NF053188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimL-related protein"
                     /protein_id="HIR5608094.1"
                     /translation="MSETPENQIVGAAPDVAPLAWVLGEIRTTLAQATVDVQTFLGAK
                     HDVEPLRGARAQIHQANGALQMLNLRGVVQVLEAVEQLLRRWETEPRECLPAAVRSVE
                     STLSAVVAYLEAMLAGRPNQPIRLFPYYREILVLSHAPRVHAADLIFPDLSRRPAFHS
                     ISIRPMTSDQLRVRRVHFESGLLGFLRNPNDVDARGMMSDALLDLENLPQRGLARSFW
                     WVVRGLMDALAADHLSIDVDLKRVMARLNLQLRRVIEGSSAVAERLMIDALYYVGRAA
                     PGEARVAEVKRLYGLDALIPPDFERATLTSIDPEQLRSLKEALTQAKTQWTPLASGMA
                     NDAAKFVHEIQIARKAAHKIGAHAIDIVLETVGSLAGDMGALSPAVRETLGIEIASAL
                     LFVDLGIEELPHTEPQFEQRARQVADRLMAARNDEPLPDLGPWLTELARKAQDKLTMS
                     TVVAETQATLREVEQRLDRFFRQPAERAELAATPPMLDQIAGVLSLLGHDDPVAAIRN
                     AQSEIARFARPDAAPSQHEFVRIANNLGAIGFFVETLSQDVDRPRGMFRWDADAGVFS
                     ADLAVAQIDEATALREAEMGPITRMGGAAGENVESSARRHVELAHGLAERLAAGDNAA
                     LREFDQILPQLTNDADLLDDDALKQRAARAAVLVKQLHEVRNANSVAELLQIFAPPPA
                     AAPAPAPAPLPVSEAAADAELLGIFVEEANDVLDTVDAQLERLAAAPADQATMTTTRR
                     AFHTLKGSSRMVGLKNFGEGAWSVEQCFNLWLAQERPATDDLIQLASAARTLMRG"
     gene            complement(2401..4590)
                     /locus_tag="AC16HB_14755"
     CDS             complement(2401..4590)
                     /locus_tag="AC16HB_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5608095.1"
                     /translation="MAMDAEMKGSSKDGESHITVQPFRYTEFGPAEVAGRARAPIPPS
                     PPTQKIDRRALEAALSSTWGSRMVDRLLTWRLPLIGNRPLNTQVQVMLTLMGVFAFLF
                     AAVLAIDVRLSASNAAQAQLLGDTLTHSQRMAKAAPRAAEGNGTALTELRESRDRVAS
                     NLESLASAVATSAVPVLEQAATTWKASEAAASQIVAQDKALRAYGDFLTRLNDAAPRL
                     AQLTDEVAAQRGSSGPAFQPALLAQRIARTAAVLASGQAQADTAPGLTRDLNQFRDLL
                     PTLNAGDAKSQQIAKLFGDLQPSIAAASGAFATVAEAGRAQAVIQRDAEALRAQLSAV
                     QGELQKEGGVRAVNFIIMAVFGLGVLLAAVGLVKILLGDSRSRTLEAEARRNEAQTLE
                     EAAKRANEQNQAAILRLMNELQEVADGNLTIEATVSEDITGAIADSVNYTVEELRGLV
                     SRINDAAEQVSSASTQAREITTALLSASEQQSREIKDTGQKVLIMASQINEASRSASE
                     SARVARASLEAAERGQRTVYNQIAGMNEIREQIQETAKRIKRLGESSQEIGEIVELIS
                     DITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLADRSAEAAKQIGALVRTIQTD
                     TQDAVTAMEKSTQGVVEGTKLSDAAGTSLAEIGRVTRQVADLIDRVAGTTGEQAQSAS
                     GVAVSIESILATTEHTSQGTRQAAQSIGQLAALARELKASVARFRVS"
     gene            complement(4627..>5068)
                     /locus_tag="AC16HB_14760"
     CDS             complement(4627..>5068)
                     /locus_tag="AC16HB_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR5608096.1"
                     /translation="GTSLRLAVRIGSRAYLVEMATAGEIVPLGEVAPVPWTKPWYRGL
                     TNVRGRLVGVIDLMQFSGGAPMTAEQGQQLLVIGDSLKSSAALIVTRAFGLRNLRELE
                     RLDEVNDSARPWELARYRDSDGTVLTELDLGRLVSFERFGAIGA"
BASE COUNT          699 a         1828 c         1695 g          846 t
ORIGIN      
        1 catccgcgca tcagggtgcg cgcagcggac gccagctgga tcagatcgtc ggtggctgga
       61 cgctcctgcg ccaaccacag attgaagcac tgttcgaccg accacgcgcc ttcgccaaaa
      121 ttcttcagcc ccaccatgcg gctggagccc ttcagcgtgt ggaacgcccg ccgcgtcgtc
      181 gtcatcgttg cctggtcggc gggcgccgcg gcgaggcgct cgagctgtgc gtcgacggta
      241 tcgaggacgt cgttggcttc ctcgacgaag atgccgagca gctcggcgtc ggccgccgct
      301 tcggaaacgg gcagcggcgc gggcgccggc gccggcgcgg ccgcgggggg cggcgcgaag
      361 atctgcagca actcagccac cgagttcgcg ttgcgtactt cgtgcagttg cttgaccagc
      421 acggcggccc gcgcggcgcg ctgtttcagc gcgtcgtcgt cgagcaggtc ggcgtcgttt
      481 gtcagctgcg gcaggatctg gtcgaactcg cgcagcgccg cgttgtcgcc tgcggccagt
      541 cgctcggcca gcccgtgcgc cagttcgacg tgacggcgcg ccgaggattc gacgttctcg
      601 cccgcagcgc cgcccatgcg cgtgatgggc cccatctctg cttcgcgcag cgccgtcgcc
      661 tcgtcgatct gcgccaccgc gaggtcggcg ctgaacacac ccgcgtccgc gtcccagcgg
      721 aacatgccgc gcggccgatc gacgtcctgg ctcagcgtct cgacgaagaa tccgatcgcg
      781 ccaaggttgt tcgcgatgcg cacgaactcg tgctggctcg gcgccgcatc cggccgcgcg
      841 aagcgcgcga tttcgctctg cgcattgcga atcgccgcca ccgggtcgtc atgtccgagc
      901 agcgacagca cgcccgcgat ctggtcgagc atcggtggcg tggcggccag ttcggcgcgt
      961 tcggccggct gccggaagaa gcggtcgagc cgctgctcga cttcgcgcag tgtggcctgc
     1021 gtttcggcca cgaccgtgct catcgtcagc ttgtcctgcg ccttgcgcgc cagttcggtc
     1081 agccacgggc cgaggtccgg cagcggctcg tcgttgcgcg cggccatcag ccggtcggca
     1141 acctgacgcg cccgctgctc gaactgcggc tccgtgtgcg gcaactcctc gatgccgaga
     1201 tcgacgaaca gcagcgccga ggcgatctcg atgccgagcg tttcgcgcac tgccggcgac
     1261 agcgcgccca tgtcgccggc cagcgaaccg acggtctcga gcacgatgtc gatcgcgtgc
     1321 gcgccgatct tgtgcgcggc cttgcgtgcg atctggatct cgtgcacgaa cttggccgcg
     1381 tcgtttgcca tcccgctcgc cagcggggtc cactgcgtct tggcctgggt gagcgcctcc
     1441 ttcagtgagc gcagctgctc cggatcgatc gatgtcagcg tggcacgctc gaaatcgggc
     1501 gggatcagcg catcgaggcc gtacagccgt ttgacctccg ccacgcgggc ctcgcccggc
     1561 gcagcgcggc cgacgtaata cagcgcatcg atcatcagcc gctcggcgac cgcgctgctg
     1621 ccctcgatca cacggcgcaa ctgcaggttc aggcgcgcca tcacgcgctt cagatcgaca
     1681 tcgatggaca ggtggtcggc cgcgagcgcg tccatcaggc cgcgcaccac ccaccagaag
     1741 ctacgcgcca gcccgcgctg cggcaggttc tccaggtcga gcagcgcgtc gctcatcatg
     1801 ccgcgcgcgt cgacatcgtt cgggttgcgc agaaagccga gcaggccgct ctcgaaatgc
     1861 acgcggcgca cgcgcagctg atccgacgtc atcggtcgga tgctgatgct gtgaaaggcg
     1921 ggacgtcggg acagatcggg gaagatcagg tccgccgcgt gcacgcgcgg cgcgtgcgac
     1981 agcacgagga tctcgcggta gtagggaaac aggcggatcg gctggttcgg ccggcccgcg
     2041 agcatcgctt ccagatacgc gaccaccgcc gacaaggtcg attcgaccga ccgcaccgcg
     2101 gccggcaggc attcgcgcgg ctcggtctcc cagcggcgca gcaactgctc gacggcttcg
     2161 agcacctgca ccacgccgcg cagattcagc atctgcagcg cgccgttggc ctggtggatc
     2221 tgcgcccgtg cgccgcgcag cggttcgacg tcgtgcttgg ccccgaggaa cgtctgcacg
     2281 tccacggtcg cctgggcgag cgtcgtgcga atctcgccca acacccacgc cagcggcgcc
     2341 acgtccggcg cggctccgac gatctggttc tccggcgtct cgctcatggc gcgctccggc
     2401 tcaggaaaca cggaagcgcg ctaccgacgc cttcagctcg cgcgccagcg ccgcgagctg
     2461 gccgatcgac tgcgctgcct gacgcgtgcc ctgcgacgtg tgctcggtgg tcgcgaggat
     2521 gctctcgatc gacaccgcga cgccgctggc ggactgcgcc tgctcgcccg tcgtgccggc
     2581 cacgcgatcg atgaggtcgg cgacctggcg cgtcacgcgg ccgatctccg ccagcgacgt
     2641 gcccgcggcg tcggacagct tcgtaccctc gaccacgcct tgcgtgctct tctccatcgc
     2701 ggtaaccgcg tcctgcgtgt cggtctggat cgtgcgcacc agtgcgccga tctgtttggc
     2761 cgcctcggcg gagcggtccg cgagccgctg cacttcttcc gcgaccacgg aaaatccgcg
     2821 accggcctcg ccggcggaag cggcctggat cgcggcgttc aacgccagca cgttggtctg
     2881 ctcggtgatg tccgagatca gttcgacgat ctcgccgatc tcctgcgacg actcgccgag
     2941 ccgcttgatc cgcttggcgg tctcctggat ctgctcgcgg atctcgttca tgccggcgat
     3001 ctggttgtag accgtgcgtt ggccgcgctc ggcagcctcg agcgaggcgc gcgccacgcg
     3061 cgccgactcg ctcgcgctgc gcgaggcctc gttgatctgc gaggccatga tcagcacctt
     3121 ctggccggtg tccttgatct cgcggctctg ctgctcggac gccgacagca gcgcggtcgt
     3181 gatctcgcgc gcctgcgtcg atgcgctgct gacctgctcg gcggcgtcgt tgatgcggct
     3241 cacgagcccg cgcagctcct cgacggtgta gttgaccgag tcggcgatcg cgccggtgat
     3301 gtcctcgctc accgtcgctt cgatcgtcag gttgccgtcg gcgacttcct gcagttcgtt
     3361 catcaatcgc aggatcgccg cctgattctg ctcgttggcg cgcttggccg cttcctcgag
     3421 cgtctgggcc tcgttgcggc gcgcctcggc ctcgagcgtg cggctgcggc tgtcaccgag
     3481 caggatcttc accagcccca ccgcggccag cagcacgccg aggccgaaca ccgccatgat
     3541 gatgaagttg acggcgcgca ccccgccttc cttctgcagt tcaccctgaa ccgcgctgag
     3601 ctgcgcgcgc agcgcctcgg cgtcgcgctg gatcaccgct tgcgcgcgtc cggcctctgc
     3661 cacggtggcg aacgcgccgc tcgccgccgc gatcgacggc tgcaggtcac cgaaaagctt
     3721 tgcgatctgc tgcgactttg catcgccggc gttcagggtc ggcagcaggt cgcggaactg
     3781 gttcaggtcg cgcgtcagcc ccggcgccgt gtcggcctgc gcctgacccg atgccagcac
     3841 ggcggcagtg cgcgcgatcc gctgggcgag caatgcgggc tggaacgccg gccccgaaga
     3901 accgcgctga gcggcgacct cgtcggtcag ttgcgccagg cgcggcgccg cgtcattcag
     3961 gcgcgtcagg aagtccccat aggcgcgcaa cgccttgtcc tgcgccacga tctgcgacgc
     4021 ggctgcctcc gatgccttcc aggtcgtcgc tgcctgctcg agcaccggca cggcggaggt
     4081 ggcgacggcg ctcgccagtg attcgaggtt ggacgcgacc cgatcgcggc tttcgcgcag
     4141 ttcggtcagg gcggtgccat tgccctcggc ggcgcgcggt gccgccttgg ccatccgctg
     4201 cgaatgcgtg agtgtgtcgc cgagcagttg tgcctgcgcg gcattgctgg cggacagccg
     4261 cacgtcgatc gccagcaccg cggcgaacag gaacgcaaac acgcccatca gggtcagcat
     4321 cacctgcacc tgcgtgttca gcgggcggtt gccgatcagc ggcagccgcc atgtcagcag
     4381 ccgatcgacc atgcgtgagc cccaggtgct ggacagcgcg gcctcgagcg cgcggcgatc
     4441 gatcttctgc gtcggcggac tcggcgggat cggcgcccgg gcacggccgg ccacttcggc
     4501 cggaccgaac tccgtgtagc ggaacggctg aacggtgata tggctttcgc cgtccttcga
     4561 cgaccccttc atctctgcgt ccatcgccat gcccatctcc gccactccgt cgtgtgtgcc
     4621 cgcgggctac gcaccgattg cgccgaaccg ctcgaacgac accaggcgcc cgaggtcgag
     4681 ttcggtcagt accgtgccgt cgctgtcgcg atagcgcgcg agttcccatg gccgcgcgct
     4741 gtcgttcacc tcgtcgaggc gctccagttc cctcagattg cgcagcccga aggcgcgcgt
     4801 gacaatcagc gccgcgctcg acttcagcga atcgccgatc accagcagct gctggccctg
     4861 ctcggcggtc atcggcgcgc cgccgctgaa ctgcatcagg tcgatcacgc cgaccaagcg
     4921 gccgcgcacg ttggtgagcc cgcgatacca gggcttggtc cacggcaccg gggccacctc
     4981 gcccagcggc acgatctcgc ccgcggtcgc catttccacc aggtaggcgc gactgccaat
     5041 gcgcaccgcc agccgcagcg acgtaccg
//
LOCUS       DBNHBY010000611         9841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_448233, whole genome shotgun sequence.
ACCESSION   DBNHBY010000611 DBNHBY010000000
VERSION     DBNHBY010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_448233"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="AC16HB_14765"
     CDS             <1..1370
                     /locus_tag="AC16HB_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5608097.1"
                     /translation="WESAARKTKDKAINILQGRAVGGSTTVNWTSSFRTPPATLAYWR
                     ERFGLRGFVPEDLAPYFEQVERRLNIAPWSGAPNANNDALKRGCVALGIDVKAMQRNV
                     NGCWDLGYCGMGCPTNAKQSMLVTTIPTALDRGARLFVRLRAVRLDLDGNRIARLACA
                     ALRDDGLTESGVAVEIRARHFVAAGGAINTPALLLRSAAPDPYGVLGQRTFLHPTVIS
                     SALFERRIDGYAGAPQTTYSDHFLETDAIDGPLGFKLEAAPLHPVLFASTLHGFGAAH
                     AQLMRRFAHAQVTLALLRDGFHPESRGGRVELRADGSPMLDYPLNEPVWNTARRALTV
                     MAEIQFAAGAQTVYPVHELATGYSTFADARQAIAQLPMRPHLTRVASAHVMGGCGMAA
                     SERAGVVDARGRHFQIENLSVHDGSIFPTSVGANPQLSIYGIVNRMAQQLAGELTLTK
                     APALD"
     gene            1399..2571
                     /locus_tag="AC16HB_14770"
     CDS             1399..2571
                     /locus_tag="AC16HB_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608098.1"
                     /translation="MPAQTDPPVPRRVLPTIVGAQLAGTSLWFAVNGVLPDLQRAVAL
                     PATALGTLTSAVQLGFITGTLTFALLTIADRFSPRLVFLLCALAGAVANALGIAFADR
                     YPALVLLRFATGFFLAGIYPVGMKIAAAWYTRGLGAALGLLTGALVLGTAMPHGLRAL
                     GADWPWQRVMLAVSLIAAGGGVVLYLLVPESPHLPRAARIRPAALAVVWRDPKVRASA
                     FGYFGHMWELYSVWALLPLIVATRTAPQSVSSASFWIIAAGSVGCAVGGWIALRLGSA
                     HVAAAQLATSGACCLLAPLALDAPLPLFWLWLAVWGVTVAGDSPQFSSLTALNSPRDA
                     VGSVLTFVNSIGFAITVVSIQLFAGAAATTPLGHLLPWLALGPLLGLYALRPLLRA"
     gene            complement(2541..4241)
                     /locus_tag="AC16HB_14775"
     CDS             complement(2541..4241)
                     /locus_tag="AC16HB_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608099.1"
                     /translation="MASLVSVRDLHVSFRLGKTHVVDAVRGVSFDVPENATVALVGES
                     GSGKSVSAMSIVRLLPDNAIVGNTSMVEFGGGNLLREPDEALRRIRGKDISVVFQEPM
                     TSLNPVFTVGDQIAEVLRLHMGLSRAQARDRVVEVLTEVGIPEPKARVASYPHELSGG
                     QQQRVMIAMAIACEPRLLIADEPTTALDVTVQRQILELIAGLQERRRMSVLFISHDLG
                     VVGEIADHVVVMRDGVVREAGGVDRIFADPQDMYTKALLACRPRLDAKPKRLPVIDDF
                     MRGSGQPIVIAEQRVARPAEPKPLIEAKGLRKEYRLKTGLLQHKTIEAVKEASFTLSR
                     GRTLGVVGESGSGKTTVGMLLMRLIDATRGQVFYDGTDLLTLSDAQMMSYRRRIQIIF
                     QNPYASLNPRFTVGQILTEPMKVHGVGAGDEDRFERAVALARRVGLGADSVHKYPHEF
                     SGGQRQRIAIARVLSMQPEVIICDESVSALDVSVQATVLNLLLDLQDEFGISYIFISH
                     DLAVVKYMADDILVMSAGEIVERGTAEAIYENPRNEYTKRLLGSIPRGYQARSSGRSA
                     "
     gene            complement(4245..5297)
                     /locus_tag="AC16HB_14780"
     CDS             complement(4245..5297)
                     /locus_tag="AC16HB_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608100.1"
                     /translation="MTAVATPYAPVESTRSEGVWRGAWRRLRHDRVGMVSLAIVIAFL
                     LLIMASALGLVARDWQKEIGVPNAPPTFVGPAPKETGTDPLAGAVPRNAKPVDLSDID
                     PLAPKYKEWAERAAKIQITEPHKAETLPFGADRLGRDVLAKSIKGAEISIFVGVFAAV
                     LATLIGTVLGALAGYAGKRVGDFLEWVYNVFTSVPDILLIFAFAAVFGRGIGTVVLIL
                     GLAGWTGMYRQVRAEYIKHRSREYVRAAEAIGVTHVARMFVHILPNVSHVVLVRMSLL
                     VVGFIKAEVILSFLGLGVPVDQVSWGTMLSEAQSELVLGHWWQLASATLFMATFVTAF
                     SLLTDALRDALDPKLR"
     gene            complement(5294..6232)
                     /locus_tag="AC16HB_14785"
     CDS             complement(5294..6232)
                     /locus_tag="AC16HB_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608101.1"
                     /translation="MTAYILRRLWQMIPTLAGVILLVFFLFKFFGGDPAEILGGLLAT
                     PEQVQAIRQQLGLNEPWYVQLWIFVRSIFSFSWGNSWATSEAVSHIFATRLPATLTVM
                     VPILLLDVTLGVCAAMAVAYVRGTLTDRLVMIITTVMLSISFLVYIIVGQWLFGFYLG
                     WFPVQGWTDSFWTNLTTYAVLPVTLAVFVGLAPQTRLYRSFFLDELGHDYVRTARAKG
                     LGENTILFKHVLRNALIPVLTNVSVQLPGIFIGSFLIETFFSIPGLGREVLLAVNRSD
                     YPVIQAVTVYLAVLTMFINLIADILYKAADPRVVLR"
     gene            6367..8196
                     /locus_tag="AC16HB_14790"
     CDS             6367..8196
                     /locus_tag="AC16HB_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608102.1"
                     /translation="MSTVSHSRRAVLQSLLALPAAANAAARAQNASRPKVLRYAFEVA
                     ETGFDPAQLSDLYSRIVTAHIFEGLYRYDYLARPYLIKPNTAAAAPEVTDDFRVWTVR
                     IRPGIYFQDDAAFNGTRRELVAADYVYAFKRFFDPRWKAPAYATLAETQIIGLDALRD
                     EVLKEKRPFDYDRPIEGLRALDRYTLQFRLAQPRPRFLQVVAGGDLYGAVAREVVEAY
                     GDDVMAHPVGTGPFQLAEWRRSSKIVLERNPSYREALWDEQPNQDDAEGRTLAQRLRG
                     KRLPLIDRVEISIVEESQPRWLSFLNRQFDLMERLPYEFVDIAVPNGRIAPNLSRQGV
                     QMARILNADVTITLFNMEHPVVGGYTPEKVALRRAINLAIDVDQEIRLFWRGQAVPAQ
                     SPVAPHLTGYDPNFVSENGEFNLARAKALLDMFGYVDRDNDGWREQPDGSLLVLEWAT
                     TPDQRARQRDELRRKDLTALGIRIEFKPAKWPENLKNARSGKLMVWSLGYSAAAPDGQ
                     PALDRGASVHVGGQNLARFRNKEFDTLYNRMRGLPDGPERERLFAEGKRMLVAYAPYK
                     YHVHRILTDLSQPWLIGYRRPLFSQEWWHYVDIDAAAQSRAIA"
     gene            8193..>9841
                     /locus_tag="AC16HB_14795"
     CDS             8193..>9841
                     /locus_tag="AC16HB_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608103.1"
                     /translation="MSRRDFLRAAGALGVAGALDGAIAADAPADRTFRWAFNAGETGF
                     DPAQINDLYSNYVVSNIFEAPLQYDFLARPALLSPRTAAALPEISSDFRTFTVRIRSG
                     IFFQDDPAFKGKRRELVAADYVYQLKRTADPRWKCPIYPSLQSAQIIGLAELRKLALD
                     TGRFDYDVEIEGIRALDRYTFQVRLAQPSPRFHENFADARPFAGVAREVVEMYADDIM
                     AHPVGTGPFRLLQWKRSSRMVLERNAAYRDEFYDAQPAADDARGQAILATLKGRKLPM
                     IDRVELSVIEESQPRWLSFLNGQQDTVNVPLEFINLAVPNGRPAPSLAKRGIEFERVI
                     NPDVTITYFNMDDPVVGGYTPDKVALRRAIALGYDIDEEIRLIRRGAAIPAQSQVPPL
                     TSGYDPAFVSEMGAFDVARARALLDTFGYVDRDGDGWRERPDGSPLILDMATQASQID
                     RAFNELWKRQMDRLQIRIEFRVNQWPENLKAGRAGKLMMWQLGNTAAAPDSDDFLAFG
                     ASRAIGGSNFARFKQPDYDRLYDRQRQLPDGPERDAVIREM"
BASE COUNT         1606 a         3436 c         3221 g         1578 t
ORIGIN      
        1 actgggaatc cgcagcgcgc aagaccaagg acaaggcgat caacatcctg caagggcgcg
       61 cggtcggcgg ctcgacgacg gtgaactgga cctcgagttt ccgcaccccg ccggcgacgc
      121 tcgcatattg gcgcgagcga ttcgggctgc gcgggttcgt acccgaagac ctcgcgccct
      181 acttcgagca ggtcgagcgc aggctgaaca tcgcaccctg gtccggcgcg ccgaacgcga
      241 acaacgatgc gttgaagcgc ggctgcgtcg cgctcggcat cgacgtgaag gccatgcagc
      301 gcaacgtgaa cggctgctgg gacctgggct attgcggcat gggctgcccg accaatgcca
      361 agcagtcgat gctggtgacg acgatcccga cggcgctcga ccgcggcgcg cgcctctttg
      421 tgcgcctgcg cgcagtgcgc ctcgacctcg acggcaaccg cattgcgcgc ctcgcgtgcg
      481 cggcgctgcg cgacgatgga ttgactgaaa gcggcgtcgc ggtcgaaatc cgggcaagac
      541 acttcgtcgc cgccggcgga gccatcaata cgccggcact gctgctgcgt tcggccgcgc
      601 ccgaccccta cggcgtgctc ggccagcgca cgttcctgca tccgaccgtc atttcgtccg
      661 cactgttcga acggcgcatc gacggctacg caggcgcgcc gcaaaccacg tactcggatc
      721 acttcctcga aaccgatgcg atcgacggtc cgctgggatt caaactggag gccgcgccgc
      781 tgcacccggt gcttttcgcg tcgacgctgc acggtttcgg cgccgcgcat gcgcagctga
      841 tgcggcggtt cgcgcatgcg caggtcactc tggccctgct gcgcgacggc ttccatccgg
      901 aaagtcgcgg cggccgcgtg gaactgcgtg ccgacggcag cccgatgctg gattacccgc
      961 tgaacgaacc ggtctggaac accgcgcggc gcgctctgac ggtaatggcg gagatccagt
     1021 tcgccgcggg cgcgcagacg gtctacccgg tgcacgaact cgcgaccggc tattcgacgt
     1081 tcgccgacgc ccggcaggcg atcgcgcagc tgccgatgcg gccgcacctg acgcgcgtgg
     1141 ccagcgcaca cgtgatgggc ggctgcggca tggcggcgtc ggagcgcgcc ggcgtagtcg
     1201 atgcgcgcgg ccgccacttc cagatcgaga acctgtcagt gcatgacggt tcgatcttcc
     1261 cgacttcggt cggcgccaat ccgcagctct cgatctacgg catcgtcaac cggatggcac
     1321 agcagctggc cggggaactg acgctgacaa aggcgcccgc gctcgactga ccgccggggc
     1381 cggactagat tcccggccat gcctgcacag acggatccgc cggtcccgcg ccgcgtgctg
     1441 ccgacgatcg tcggcgcaca actcgccggc acgtcgcttt ggttcgcagt caacggtgtt
     1501 ctgcccgatc tgcagcgtgc ggtcgcgctg cccgccaccg cgctcggcac gctgacttct
     1561 gcggtgcagc tcgggttcat cacgggcacg ctgacgttcg cgctgctgac gatcgccgac
     1621 cgtttttccc cgcggctggt gttcctgctg tgcgcgctcg cgggcgctgt ggccaacgcg
     1681 ctcggcatcg cgtttgccga caggtatccg gcgctggtcc tgctgcgctt cgccaccggc
     1741 ttctttcttg ccggcatcta tccggtcggc atgaagatcg ccgcggcatg gtatacgcgt
     1801 gggctcggcg ccgcgctcgg cttgctgacc ggcgcgctcg tgctcgggac cgcgatgccg
     1861 cacggactgc gcgcgctcgg cgccgactgg ccgtggcaac gggtgatgct cgccgtatcg
     1921 ctgatcgcgg ccgggggcgg cgtcgtgctg tacctgctcg ttcccgagag tccgcacctg
     1981 ccgcgcgccg cgcgcatccg gccggcggcg ctggcggtgg tctggcgcga cccgaaggtg
     2041 cgcgcgtcgg cgttcggcta tttcggccac atgtgggagc tgtattcggt ctgggcgctg
     2101 ctgccgctga tcgttgccac acgcaccgcc ccgcagtccg tctcatccgc ctcgttctgg
     2161 atcatcgccg cggggtccgt cggctgcgct gtcggtggct ggatcgcact gcgcctcggc
     2221 agtgcgcacg tggccgccgc gcagctggcc accagcgggg cgtgctgcct gctcgcgccg
     2281 ctcgcgctgg acgcgccgct gccgctcttc tggctgtggc tcgccgtgtg gggcgtcacc
     2341 gtggccggcg actcgccgca gttctcctcg ctgaccgcgc tgaattcgcc gcgcgacgcg
     2401 gtcggcagcg tgctcacgtt cgtcaacagc atcggcttcg cgatcacggt cgtcagcatc
     2461 cagctgttcg ccggcgccgc ggcgaccacc ccgctcggcc atctgctgcc gtggctcgcg
     2521 ctcgggccgc tgctcgggct ttacgcgctg cggccgctgc tgcgcgcctg atagccgcgc
     2581 gggatcgacc cgagcagccg cttcgtgtac tcgttccgcg gattctcgta gatcgcctcg
     2641 gccgtgccgc gctcgacgat ctcgcccgcg ctcatcacca ggatgtcgtc ggccatgtac
     2701 ttgacgaccg ccagatcgtg gctgatgaag atatagctga tgccgaactc gtcctgcagg
     2761 tcgagcagca ggttcagcac cgtggcctgc acagaaacat cgagcgcgga gaccgactcg
     2821 tcgcagatga tcacctcggg ctgcatcgac agcacgcgcg cgatcgcgat ccgctgccgt
     2881 tgcccgccgg agaactcgtg cggatacttg tgcacgctgt cggcgccgag gccgacgcgc
     2941 cgcgccagcg cgaccgcgcg ctcgaagcgg tcttcgtctc cggcgccgac gccgtgcact
     3001 ttcatcggtt cggtcaggat ctgaccgacg gtgaagcgcg gattcagcga cgcgtacggg
     3061 ttctggaaga tgatctggat gcggcgccgg tacgacatca tctgcgcgtc cgaaagcgtc
     3121 agcaggtcgg tgccgtcgta gaacacctgc ccgcgcgtgg catcgatcaa ccgcatcagc
     3181 agcatgccga ccgtcgtctt gcccgagcca gactcgccga ccacgcccag cgtgcgcccg
     3241 cgcgacagcg tgaacgacgc ctccttcacc gcctcgatcg tcttgtgctg cagcagcccg
     3301 gtcttcagcc ggtactcctt gcgcagccct ttggcctcga tcaacggctt cggctcggcg
     3361 ggccgcgcga cgcgctgctc ggcgatcacg atcggctggc cgctgccacg catgaaatcg
     3421 tcgatcaccg gcaaccgctt cggtttcgcg tcgagccgcg gtcggcaggc gagcagtgcc
     3481 ttcgtgtaca tgtcctgcgg gtcggcgaag atgcgatcga ccccgcccgc ctcgcgtacc
     3541 acgccgtcgc gcatcacgac gacatggtcg gcgatctcgc cgacgactcc gaggtcgtga
     3601 ctgatgaaga gcaccgacat gcgccgtcgc tcctgcaggc cggcgatcag ttcgaggatc
     3661 tgccgctgca ccgtcacgtc gagggcggtg gtcggttcgt cggcgatcag cagcctgggc
     3721 tcgcacgcga tcgccatcgc aatcatcacg cgctgctgct gcccgccgga cagttcgtgc
     3781 gggtagctcg cgacgcgtgc cttcggctcc ggaatgccga cctcggtcag cacctcgacc
     3841 acgcgatcgc gcgcctgcgc gcgcgaaagc cccatgtgca gccgcagcac ctcggcgatc
     3901 tggtcgccga ccgtgaatac cgggttgagc gacgtcatcg gctcctggaa caccacgctg
     3961 atgtccttgc cgcggatccg gcgcaaggct tcatcgggct cgcgcaacag gttgcccccg
     4021 ccgaactcga ccatgctcgt gttgccgacg atcgcgttgt ccggcagcag ccgcacgatc
     4081 gacatcgccg acacgctctt gcccgagccc gattcgccga ccagcgcgac ggtcgcgttc
     4141 tccggcacgt cgaaactgac gccgcggacc gcgtcgacca cgtgcgtctt gccgagccgg
     4201 aacgacacgt gcaggtcgcg caccgaaacc aggctagcca tcggctatct cagcttcgga
     4261 tcgagcgcat cgcgcagcgc gtcggtcagc agcgaaaatg cggtgacgaa cgtcgccatg
     4321 aagagcgtgg ccgacgcgag ctgccaccag tggccgagca ccagttcgga ttgcgcctcc
     4381 gacagcatcg tgccccacga cacctgatcg accggcacgc cgagcccgag gaacgacagg
     4441 atcacctcgg ccttgatgaa gccgaccacc agcagcgaca tgcgcaccag caccacgtgg
     4501 ctgacattag gcaggatgtg cacgaacatg cgcgcgacat gcgtcacgcc gatcgcctcg
     4561 gccgcgcgca cgtactcgcg cgagcgatgc ttgatgtact ccgcgcgcac ctggcgatac
     4621 atgccggtcc agcctgccag acccagtatc agcacgaccg tgccgatacc gcgcccgaac
     4681 actgcggcga acgcgaagat cagcagtatg tcgggcaccg acgtgaagac gttgtagacc
     4741 cactcgagga agtcgccgac acgtttgccc gcgtagcccg ccagcgcgcc gagcacggtg
     4801 ccgatcagcg tggcgagcac ggcggcgaac acgccgacga agatcgagat ctcggcaccc
     4861 ttgatcgact tcgccagcac gtcgcgcccg agccggtcgg cgccgaacgg cagcgtctcg
     4921 gccttgtgcg gctcggtgat ctggatcttc gccgcgcgct cggcccactc cttgtacttc
     4981 ggcgccagcg ggtcgatgtc ggacagatcc accggcttcg cattcctggg aaccgcgcct
     5041 gcgagcggat cggtcccggt ttccttcggc gccggtccca cgaatgtcgg cggcgcgttc
     5101 ggcacgccga tctccttctg ccagtcgcgc gccacgagtc cgagcgcgga ggccatgatc
     5161 agcagcagga acgcgatcac gatcgccagc gacaccatac cgacccgatc gtgccgcagc
     5221 cggcgccacg cgccgcgcca cacgccttcg gagcgcgtcg attcgaccgg cgcgtacggc
     5281 gtggcgaccg ccgtcatcgg agcaccacgc gcggatcggc tgctttgtaa aggatgtcgg
     5341 cgatcaggtt gatgaacatc gtcagcacgg cgagatacac cgtgaccgcc tggatcaccg
     5401 ggtagtcgct gcggttgacc gccagcagca cctcgcgccc gaggcccggg atcgagaaga
     5461 acgtctcgat caggaacgag ccgatgaaga tccccggcag ctgcacgctc acgttggtca
     5521 gcaccgggat cagcgcgttg cgcagcacgt gcttgaacag gatcgtgttc tcgccgaggc
     5581 ccttggcgcg cgcggtgcgc acatagtcgt ggccgagttc gtcgaggaag aaactgcggt
     5641 aaagccgcgt ctgcggcgcg agccccacga acaccgcgag cgtcaccggc agcaccgcgt
     5701 aggtggtgag gttggtccag aagctgtcgg tccaaccctg caccgggaac cagccaaggt
     5761 agaaaccgaa cagccactgg ccgacgatga tgtagacgag gaacgagatc gacagcatca
     5821 ccgtcgtgat gatcatcacg aggcgatcgg tcagcgtgcc gcgcacgtac gcgaccgcca
     5881 tcgccgcaca cacgcccagc gtcacatcga gcagcaggat cgggaccatc accgtcaacg
     5941 tcgccggcag ccgggtcgcg aagatatggc tcaccgcctc gctggtcgcc cacgaattgc
     6001 cccagctgaa gctgaagatc gatcgcacga agatccacag ctgcacgtac cacggctcgt
     6061 tcaggccgag ctgctggcgg atcgcctgga cctgctccgg cgtggcgagc agtccgccca
     6121 ggatctcggc cggatcgccg ccgaagaact tgaacaggaa gaacaccagc aggatgacgc
     6181 ccgccagcgt cgggatcatc tgccaaagcc tgcgtaagat gtacgcggtc acgtcgtctg
     6241 cccgcctgag cgttgcggtc cgatgctctg cagcgtgcgc gcgtggcccg cgccgcacga
     6301 aaggccggca ttatgccgag ccccgtgctg caatcaaacg gaatcgagtt tcaccgcccg
     6361 catcgcatgt ccaccgtctc gcactcccga cgcgcggtac tgcagtcgtt gctcgcgctg
     6421 cccgcggctg ccaacgccgc ggcgcgcgcg cagaacgcgt cacgccccaa ggtgctgcgc
     6481 tacgcgttcg aggtcgccga gaccgggttc gatcccgcgc aactgtcgga cctgtattcg
     6541 cgcatcgtca ccgcgcacat cttcgagggg ctgtaccgct acgactacct ggcacggcca
     6601 tacttgatca agccgaacac ggcggctgcg gctcccgaag tgacggacga cttcagggtc
     6661 tggactgtcc gcattcggcc cggcatttat ttccaggacg acgccgcatt caacggcacc
     6721 aggcgcgaac tggtcgccgc cgattacgtc tatgcgttca agcgcttctt cgacccgcgc
     6781 tggaaggcgc cggcctacgc gacgctggcc gagacgcaga tcatcggcct cgacgcgctg
     6841 cgcgacgagg tgctgaagga aaagcgaccg ttcgactatg accgaccgat cgagggactg
     6901 cgcgcgctcg accgctacac gctgcagttc cggctcgcgc agccgcgacc ccgctttctg
     6961 caggtggtcg ccggcggcga cctgtacggc gcggtcgcac gcgaagtggt cgaagcgtac
     7021 ggcgacgacg tcatggcgca tccggtcggc accgggccgt tccagctggc cgagtggcgg
     7081 cgctcgtcga agatcgtcct cgagcgcaat ccctcctacc gcgaagccct gtgggacgag
     7141 cagccgaacc aagatgacgc cgagggccgc acgcttgcgc agcggctgcg cgggaaacgg
     7201 ctgccgttga tcgatcgcgt ggagatctcg atcgtcgagg aatcgcagcc gcgctggctg
     7261 tccttcctga accggcagtt cgacctgatg gagcggctgc cgtacgagtt cgtcgacatt
     7321 gcggtgccga atggccggat cgcgccgaac ctgtcgcgcc agggcgtgca gatggcgcgc
     7381 atcctgaatg ccgacgtcac gatcacgctg ttcaacatgg agcacccggt cgtcggcggc
     7441 tacacgccgg agaaggtcgc gttgcgccgc gcgatcaatc tggcgattga cgtcgaccag
     7501 gagattcgcc tcttctggcg agggcaggcg gtgcccgcgc agtcgccggt cgcgccgcac
     7561 ctgaccggtt acgacccgaa cttcgtcagt gagaatggcg agttcaacct ggcgcgcgcg
     7621 aaggcgctgc tcgacatgtt cggctacgtc gaccgcgaca acgacggctg gcgcgaacag
     7681 ccggacggct cgctccttgt gctcgaatgg gccacgaccc cggatcagcg cgcgcggcag
     7741 cgggatgaac tgcgtcgcaa ggacttgacc gcgctcggca tccgcatcga attcaagccg
     7801 gccaagtggc cggagaatct gaagaacgcg cgcagcggca agttgatggt ctggagcctc
     7861 ggctactccg ccgccgcgcc cgacggtcag ccggccctcg atcgcggcgc gtcggtacac
     7921 gtcggcggcc agaaccttgc gcggttccgc aacaaggagt tcgacacctt gtacaaccgg
     7981 atgcgcggtc tgccagacgg ccccgagcgc gagcgcctgt tcgccgaagg caagcggatg
     8041 ctggtcgcct atgcgccgta caaatatcac gtgcaccgca tcctcaccga tctgtcgcag
     8101 ccgtggttga tcggctaccg acggccgctc ttttcccagg agtggtggca ctacgtcgac
     8161 atcgacgccg ccgcgcagtc gagggcgatc gcatgagccg gcgcgacttc cttcgtgccg
     8221 cgggcgcgct gggcgtggcc ggcgcgctcg acggtgcgat cgcggccgat gcgcccgcgg
     8281 accgcacgtt ccgctgggca ttcaacgccg gcgagaccgg gttcgacccg gcgcagatca
     8341 acgacctgta ttcgaactac gtggtctcga acatcttcga ggcgccgctg cagtacgact
     8401 tcctggcgcg gccggcgctg ctgagtccgc gcaccgcggc cgcactgccg gagatctcca
     8461 gcgacttccg caccttcacc gtccgcatcc gatcgggcat cttcttccag gacgaccccg
     8521 cgttcaaggg caagcggcgc gaactggtcg cggccgacta cgtctatcag ctgaagcgca
     8581 cggccgatcc gcgctggaag tgcccgatct acccgagcct gcagagcgca cagatcatcg
     8641 gcctggcgga gctgcgcaag ctggcgctcg acaccggccg cttcgactac gacgtcgaga
     8701 tcgagggcat ccgcgcgctc gaccgctaca cgttccaggt ccggctggcg cagccgagtc
     8761 cgcgcttcca cgagaacttc gccgacgcgc ggccgtttgc aggcgtggcg cgcgaagtcg
     8821 tggagatgta cgccgacgac atcatggcgc atccagtcgg taccgggccg ttccgtctcc
     8881 tgcagtggaa gcgctcgtcg cgcatggtgc tcgaacgcaa cgctgcgtat cgcgacgagt
     8941 tctacgacgc gcagccggcc gcggacgatg cgcgcggcca ggcgatcctc gccacgctga
     9001 aaggccgcaa gctgccgatg atcgaccgcg ttgaactgtc cgtgatcgag gaaagccagc
     9061 cgcggtggct atcgttcctc aacggccagc aggacaccgt gaacgtgccg ctggaattca
     9121 tcaacctcgc cgtgcccaac ggccggccgg cgccgtcgct ggccaagcgc ggcatcgagt
     9181 tcgagcgcgt gatcaatccg gatgtgacga tcacctactt caacatggat gatccggtgg
     9241 tcggcggcta cacgccggac aaggtcgcgc tgcgccgggc gatcgcgctc ggctacgaca
     9301 tcgacgagga gatccgcctg atccggcgcg gcgcggcgat cccggcgcag tcgcaggtgc
     9361 cgccgctgac ctccggatac gacccggcgt tcgtcagcga gatgggcgca ttcgacgtcg
     9421 cgcgcgcgcg cgcgttgctc gacacgttcg gttacgtcga tcgcgacggt gacggctggc
     9481 gcgagcgccc ggacggctcg ccattgattc tcgacatggc cacgcaggcg agccagatcg
     9541 accgcgcgtt caacgaattg tggaaacgcc agatggaccg gctgcagatc cgcatcgagt
     9601 tcagggtcaa ccagtggccg gagaacctga aggcgggccg ggccggcaag ctgatgatgt
     9661 ggcaactggg caatacggcc gcggcgccgg attcggacga tttcctcgcg tttggcgcca
     9721 gccgcgcgat cggcggctcc aacttcgcgc gcttcaagca gcccgactat gaccgcctgt
     9781 acgaccgcca gcgccagctt ccggacggac cggaacgcga cgcggtgatc cgcgaaatga
     9841 a
//
LOCUS       DBNHBY010000612         2854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1598474, whole genome shotgun sequence.
ACCESSION   DBNHBY010000612 DBNHBY010000000
VERSION     DBNHBY010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1598474"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="AC16HB_14800"
     CDS             <1..636
                     /locus_tag="AC16HB_14800"
                     /inference="COORDINATES: protein motif:HMM:NF042682.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HIR5608104.1"
                     /translation="DRVRRLTDYHDARYAARFAALVQRTRAAQARAGIADDALARAVA
                     SGFFRLLAVKDEYEVARLYTDGEFAAELERRFEPGYRLRYHLAPPLLARTDRATGRPR
                     KIVFGPWLHAAMWLLARLRRLRGTALDPFGYTHERTIERQLANDYERTLDRLLGQMTA
                     ENCALVLEYAELPERMRGFGAVKQRNVDAALKRADEIVARVGTPPAQATQR"
     gene            633..2036
                     /locus_tag="AC16HB_14805"
     CDS             633..2036
                     /locus_tag="AC16HB_14805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5608105.1"
                     /translation="MTADMFGAVDAAFLVELRAIFGARVDTAAAIREQHGRDESYHPA
                     HAPDVVVHAHSTDEVSALMRLCSARRVPVVPYGAGTSLEGHIAAVHGGVCVDLSQMDR
                     VLAVRADDLDATVQPGVTREALNRELRHTGLFFPVDPGANATLGGMAATRASGTTAVR
                     YGTMRENVLALQVVLASGAVLRTGTRARKSAAGYDLTRLFVGAEGTLGIVTELTVRLH
                     GVPESIAAAVVPFASVQGAVQAVIAVMQLGIALSRIEFLDERQIAALNRYSQRDDPLQ
                     PTLFIEFGGMPAAVAEQVDAVRSICAEHGGAAFRWASDEAERARLWEARHKAAYAALA
                     LRPGARAWATDVCVPISRLAECIDAARQEAALAPFPTMVVGHVGDGNFHLPMLVDESN
                     AAEMNAALRLNERVVQSALAMDGTCTGEHGIGLGKREFLLAEHGAVAIDVMRSIKRAL
                     DPLGILNPGKLFVERQP"
     gene            2077..>2854
                     /locus_tag="AC16HB_14810"
     CDS             2077..>2854
                     /locus_tag="AC16HB_14810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR5608106.1"
                     /translation="MDNSTLKNFGSFDYVIVGAGTAGCLLANRLSADPEVSVLLIEAG
                     GRDDWIWIHIPVGYLYCINNPRTDWCYRTEPCDGLNGHSIIYARGKVLCCCSSINAMI
                     YMRGQKRDYDEWAALTGDASWAWDSVLPLFRRSEDHWRGADEAHGTGGAWRVEQQRLE
                     WEILNAFRAAMAETGIPLTDDFNRGDNEGAGKFEVNQRRGVRLNTSKAFLHPIAHRKN
                     LTVITDTLVTRLRLQGKRAVGVEFVRGSEAQFAEARLETVM"
BASE COUNT          436 a          984 c         1023 g          411 t
ORIGIN      
        1 gaccgcgtgc ggcgcctgac cgattatcac gatgcacgct acgccgcgcg cttcgccgcg
       61 ctggtgcagc ggacgcgcgc cgcgcaggcg cgcgctggca tcgcagacga cgcgctcgcg
      121 cgcgcggtcg cttccggctt cttccggctg ctggcggtca aggacgaata cgaagtcgcg
      181 cgcctctaca cggacggcga gttcgcggcg gagctcgaac gccgcttcga gcccggttac
      241 cggctgcgct accacctcgc gccgccgctg ctggcgcgca ccgaccgcgc gaccggtcgg
      301 ccgcgcaaga tcgtcttcgg gccgtggctg cacgccgcga tgtggttgct cgcgcggcta
      361 cgccggctgc gcggcaccgc gctagacccg ttcggctaca cgcacgagcg cacgatcgag
      421 cggcaactgg cgaacgacta cgagcgcacg cttgatcgtc tgctcggcca gatgacggcc
      481 gagaattgcg cgctggtgct cgaatacgcc gagctgcccg agcggatgcg cggcttcggc
      541 gcggtcaagc agcgcaatgt cgacgccgcg ctgaagcgcg cggacgagat cgtggcgcgc
      601 gtcggcaccc cgccggcgca ggcaacgcaa cgatgaccgc cgatatgttc ggagccgtcg
      661 acgctgcatt cctcgtcgaa ctgcgcgcga tctttggtgc acgcgtcgat accgcggccg
      721 cgatccgcga gcagcacggg cgcgacgagt cgtaccaccc ggcgcacgcg cccgacgtcg
      781 tcgtgcacgc gcattcgacc gacgaggtca gcgcgctgat gcggctgtgc tcggcgcggc
      841 gcgtgccagt cgtgccgtac ggcgccggca cgtcgctgga agggcacatc gccgcggtgc
      901 acggcggcgt gtgcgtcgac ctgtcgcaga tggaccgcgt gctcgcggtc cgcgccgatg
      961 atctggatgc gaccgtgcag cccggcgtca cgcgcgaagc gctcaaccgc gaattgcggc
     1021 atacgggcct gttcttcccg gtcgatcctg gcgccaacgc gacgctcgga ggcatggccg
     1081 cgacgcgcgc gtcgggcacg accgcggtgc gctacggcac gatgcgcgag aacgtgctcg
     1141 cgctgcaggt ggtgctcgcc tccggcgcgg tgctgcgcac cggcacgcgc gcacgcaagt
     1201 cggcggccgg ctacgacctg acgcggctct tcgtcggagc cgagggcacg ctgggcatcg
     1261 tcaccgaact gacggtgcgg ttgcatggcg tgccggaatc gatcgccgcc gccgtggtgc
     1321 cgttcgccag cgtgcagggc gcggtgcagg cggtgatcgc ggtgatgcag ctcggcattg
     1381 cactttcgcg tatcgagttc ctcgacgaac ggcagatcgc cgcgctcaat cgctacagcc
     1441 agcgcgacga cccgctgcag ccgacgctct tcatcgagtt cggcggcatg ccggcggcgg
     1501 tcgccgagca ggtcgacgcg gtgcgatcga tctgcgccga gcacggcggc gcggcgttcc
     1561 gctgggcgag cgacgaggcc gagcgcgcgc gcctgtggga ggcgcggcat aaggccgcct
     1621 acgccgcgct ggcgctgcgg cccggcgcac gcgcctgggc caccgacgtg tgcgtgccga
     1681 tctcgcggct cgccgagtgc atcgacgcgg cgcgtcagga agccgcactg gcgccgttcc
     1741 cgacgatggt cgtcggccat gttggcgacg gcaactttca cctgccgatg ctggtcgacg
     1801 agagcaacgc ggccgagatg aacgccgcgc tgcgactgaa cgaacgcgtg gtgcagagcg
     1861 cgctcgcgat ggacggcacc tgcaccggcg agcacggcat cggcctgggc aagcgcgaat
     1921 tcctgctcgc cgagcacggc gcggtcgcga tcgacgtgat gcggtcgatc aagcgcgcgc
     1981 tcgacccgct cggcatcctg aacccgggca agctgttcgt cgaacgccag ccgtgatacc
     2041 gcggcggcgg agccgcctcg agaggagtaa agggccatgg acaactcgac gcttaagaac
     2101 ttcggcagct tcgactacgt gatcgtcggc gcgggcaccg ccggctgcct gctcgccaat
     2161 cgcctgtcgg ccgatccgga ggtgtcggtg ctgctgatcg aagccggcgg ccgcgatgac
     2221 tggatctgga tccacatccc ggtcggctat ctctactgca tcaacaaccc gcgcacggac
     2281 tggtgctacc gaaccgagcc gtgcgacggg ctcaatggcc actcgatcat ctacgcgcgc
     2341 ggcaaggtac tctgctgctg ctcgtcgatc aacgcgatga tctacatgcg cgggcagaag
     2401 cgcgactacg acgaatgggc cgcgctcacg ggcgacgcgt cctgggcgtg ggacagcgtg
     2461 ctgccgcttt tcaggcgcag cgaggaccac tggcgtggcg ccgacgaggc gcacggcacc
     2521 ggtggcgcgt ggcgcgtcga gcagcagcgc ctcgagtggg agatcctgaa cgcgttccgc
     2581 gccgcgatgg cggagaccgg cattccgctg acggacgact tcaaccgcgg cgacaacgag
     2641 ggcgccggga agttcgaggt caaccagcgc cgcggcgtgc ggctgaacac gtcgaaggcg
     2701 ttcctgcatc cgatcgcgca ccggaaaaat ctcaccgtca tcaccgacac gctcgtcacg
     2761 cggctgcgac tgcaaggcaa gcgcgcggtc ggcgtcgagt tcgtgcgcgg cagcgaggcg
     2821 cagtttgccg aggcacgcct ggagaccgtg atgg
//
LOCUS       DBNHBY010000613         2351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1869766, whole genome shotgun sequence.
ACCESSION   DBNHBY010000613 DBNHBY010000000
VERSION     DBNHBY010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1869766"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="AC16HB_14815"
     CDS             <1..90
                     /locus_tag="AC16HB_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5608107.1"
                     /translation="HGRRIEAATKLGVGRNTITRKIQDLGLED"
     gene            304..1485
                     /locus_tag="AC16HB_14820"
     CDS             304..1485
                     /locus_tag="AC16HB_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608108.1"
                     /translation="MSTRMKWLLIGIALLITGGGTTAVVLKSGKFAPAAKKEVPPLEF
                     AARDLVQVTPRKLATKLSFPGNVQAVSQATVRSKLSAEVRRVLVREGDRVSAGQVVAE
                     FDTSQLRAQLAERVATLESARAQMAQSERTREANAQLIKQNFISQNAMDTADAALRAQ
                     AAAVEAAQAQLAQMQLQLADAVVRAPIAGEVAKRHVQPGEKVAFDAPLLTLVDLADLE
                     VQAQAPVSDVAQIARGAPVEIEIEGLAGRSFTASVDRINPAADPGSRTIHLYVRLPNE
                     RVDNGWLLRAGMFARVRMQVGAEREVPALPLSAVRTDDGQPSVWIIADGKLVRRAVEV
                     GRRDERAQLVEIVSGLAPADRVLVTKFDNLREGLAARVVASPAEAKVADKDAPRPPAS
                     N"
     gene            1507..>2351
                     /locus_tag="AC16HB_14825"
     CDS             1507..>2351
                     /locus_tag="AC16HB_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608109.1"
                     /translation="MWMTRVSINHPVFATMVMVALTVLGLFSYAKLGVEPMPDISPPG
                     VQIWINYPGASPEQVENDLAKPVENAVNTVAGVKRILSRSDEGRSLTWVEFRLDVDVT
                     RAMQEVRDKLAQIRASFPRDAKDPIVTRAETENDQPVASFALLGTGRSQRELTLVAEQ
                     IVQKGFERINGVGRVDLGGTVTRQVQARVDPAKLTALGLTVDQVVQALRTANVSVPVG
                     TISNPTSEAIVRVDGRLSTPLDFNRIIVARRNGAPILLSQVAEVVDGEAERQSLTRID
                     GQPAIG"
BASE COUNT          375 a          781 c          856 g          339 t
ORIGIN      
        1 cacgggcgcc ggatcgaggc ggccaccaag ctcggcgtcg gccgcaacac gatcacgcgc
       61 aagatccagg atctcggcct cgaagactga ttcgccgtcg cccgggcgcg ggccgggacc
      121 cagattcgat ccattcctgc cgggctccgg cccgcggcgg ggcgacgcta cgcttgcccc
      181 ggccccatcc gtcacgattg caactcgtcg caacatttcg cagttcgtcg caaaggacgg
      241 gcagccgtcg acgttccgct gataatcgcg cccactgaag aatcggcgcg gggaggggga
      301 ccgatgtcca cgcgaatgaa gtggctgctg atcggcatcg cactgctgat cacgggcggc
      361 ggcacgacgg cggtggtgct caagtcgggc aagttcgcgc cggcggcgaa gaaggaggtg
      421 ccgccgctcg agttcgcggc gcgcgacctc gtgcaggtca cgccgcgcaa gctggcgacc
      481 aagctgtcgt tcccgggcaa cgtgcaggcg gtatcgcagg cgaccgtgcg ctcgaagcta
      541 tcggccgagg tgcggcgcgt gctggtgcgc gagggcgacc gcgtcagcgc cggacaggtc
      601 gtcgccgagt tcgatacctc gcaactgcgc gcgcaactgg ccgagcgcgt cgccacgctc
      661 gaatcggcgc gggcgcaaat ggcgcagtcc gagcgcacgc gcgaggccaa tgcgcagctg
      721 atcaagcaga acttcatctc gcagaacgcg atggatactg cggacgccgc gctgcgcgcg
      781 caggccgccg cggtcgaggc cgcgcaggcg caactggcgc agatgcagtt gcagctcgcc
      841 gacgccgtcg tgcgcgcgcc gatcgcgggc gaggtcgcca aacgccacgt gcagccgggc
      901 gagaaggtcg cgttcgatgc gccgctgctg acgctcgtcg atctcgccga tctcgaagtg
      961 caggcgcagg cgccggtgtc ggacgtggcg cagatcgcac gcggcgcgcc ggtggagatc
     1021 gagatcgaag gcctggcggg gcgcagcttc acggccagcg tcgatcgcat caaccccgct
     1081 gccgatcccg ggtcgcgcac gatccacctg tacgtgcgat tgccgaacga gcgcgtcgac
     1141 aacggctggc tgttgcgcgc cggcatgttc gcgcgcgtgc ggatgcaggt gggtgctgag
     1201 cgcgaagtgc cggcactgcc gttgtcggca gtccgtaccg acgacggtca gccgagcgtg
     1261 tggatcatcg ccgacggcaa gctcgtgcgg cgtgcggtcg aggtgggccg ccgcgacgag
     1321 cgcgcgcagt tggtcgagat cgtcagcggc ctggcgccgg ccgaccgtgt gctcgtgacc
     1381 aagttcgaca acctgcgcga ggggctcgcc gcgcgcgttg tcgccagtcc ggcggaggcg
     1441 aaagtcgccg acaaggacgc gccgcgtccg ccggcgtcga actgatcgca gccgcgagtt
     1501 cgtgccatgt ggatgacgcg cgtttcgatc aatcacccgg tgttcgcgac catggtgatg
     1561 gtcgcactca cggtgctggg gctcttctcg tacgcgaagc tgggtgtcga gccgatgccg
     1621 gacatcagtc caccgggcgt gcagatctgg atcaactacc cgggcgcgtc gcccgagcag
     1681 gtcgagaacg atctggccaa accggtcgag aatgcggtca acacggtcgc aggcgtcaag
     1741 cgcatcctgt cgcgctcgga cgagggtcgt tcgctgacgt gggtcgagtt ccggctggac
     1801 gtcgacgtca cgcgcgcgat gcaggaggtg cgcgataagc tggcgcagat ccgcgcgagc
     1861 tttccgcgcg acgccaagga tccgatcgtc acgcgcgcgg agaccgagaa cgaccagccg
     1921 gtcgcgtcgt tcgctctgct cggaacgggt cgcagccagc gcgagttgac gctggtcgcc
     1981 gagcagatcg tgcagaaggg cttcgagcgc atcaacgggg tgggccgcgt cgacctcggc
     2041 ggcaccgtca cgcggcaggt ccaggcgcgc gtcgatccgg ccaagctgac ggcgctgggc
     2101 ctgaccgtcg atcaggtcgt gcaggcgctg cgcaccgcca acgtcagcgt gccggtcggc
     2161 acgatctcga atccgacctc ggaggcgatc gtgcgcgtcg acggacgcct gtcgacgccg
     2221 ctcgacttca accggatcat cgtcgcgcgc aggaacggcg cgccgatcct gctgtcgcag
     2281 gtggccgagg tcgtcgacgg cgaggccgag cggcagtcgc tcacccgcat cgacggccag
     2341 ccggcgatcg g
//
LOCUS       DBNHBY010000614         1530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_246645, whole genome shotgun sequence.
ACCESSION   DBNHBY010000614 DBNHBY010000000
VERSION     DBNHBY010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_246645"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="AC16HB_14830"
     CDS             <1..1096
                     /locus_tag="AC16HB_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5608110.1"
                     /translation="AKAAELWNMFLPGLRPDEPGTRMSNLDYAPMAEIMGRIPWSSEV
                     FNCNAPDTGNIELLHLAATPAQRERWLQPLLNGEIRSCFAMTEPDVASSDATNIRTSI
                     RRDGDSYLINGRKWFITGAAHPNCRIAIVLGVTDERPDAEPHRRHSMVLVPMDAPGLT
                     VVRNIPIMHHHSPDGHCELLFRDVRVPASNLLGEEGQGFKLAQARLGPGRVHHCMRTI
                     GQCELAMELMCERALERRTFGKYLADFANVQDWIAQSRIEIDQARLLVLRAAWLMDRQ
                     GNAAARVDVSAIKVVAAQLQTRVVDRAMQVFGAMGLTPDVPLSYLWTWGRALHFVDGP
                     DEVHLRTVARAELGKAKASRGSTAPYYRLG"
     gene            1116..>1530
                     /locus_tag="AC16HB_14835"
     CDS             1116..>1530
                     /locus_tag="AC16HB_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608111.1"
                     /translation="MPSWQDAASPMRREAHFGDRVVRCFAERPRDLNAMFAQAVALYG
                     AGEALIDGDRRLTWSALDDEVARVAAGLAQRGVERGERLALLLGNRAEFVIATLAAAR
                     LGAIVVPLSIRAQAPELAYMLEHSGARLVVHEAGLA"
BASE COUNT          232 a          518 c          551 g          229 t
ORIGIN      
        1 ggcgaaggcg gcggagctgt ggaacatgtt cctgccgggc ctgcggcccg acgagcccgg
       61 cacgcggatg tcgaacctcg actacgcgcc gatggccgag atcatgggcc gcatcccctg
      121 gtcgtcggag gtcttcaact gcaatgcgcc cgacaccggc aacatcgagc tgctgcatct
      181 cgccgcgacg cccgcgcagc gcgagcggtg gctgcagcct ctgttgaacg gcgagatccg
      241 ctcgtgtttc gcgatgaccg agccggacgt cgcctcgtcg gatgcgacca acatccgcac
      301 cagcatccgg cgcgacggcg attcctattt gatcaacgga cgcaagtggt tcataaccgg
      361 cgccgcgcac ccgaactgca ggatcgccat cgtgctcggc gtgaccgacg agcggcccga
      421 cgccgagccg caccgccgcc actcgatggt actggtgccg atggatgcgc cggggctcac
      481 ggtcgtgcgc aacatcccca tcatgcatca ccattcgccc gacgggcatt gcgagctgct
      541 gttccgcgac gtgcgcgtgc cggcgtcgaa cctgctgggc gaggaagggc agggcttcaa
      601 gcttgcgcag gcgcggctcg gcccggggcg cgtgcatcac tgcatgcgca cgatcggcca
      661 gtgcgagctc gccatggagc tgatgtgcga acgcgcgctc gagcgccgca cgttcggcaa
      721 gtacctcgcg gacttcgcca acgtgcagga ctggatcgcg caatcgagga tcgagatcga
      781 ccaggcacgc ctgctggtgc tgcgcgccgc atggctgatg gacaggcagg gcaatgcggc
      841 ggcgcgcgtc gatgtatcgg cgatcaaggt ggtcgccgcg cagctgcaga cccgcgtcgt
      901 cgaccgcgcg atgcaggtct tcggcgcaat ggggttgacg cctgacgtgc cgttgtcgta
      961 cctgtggacg tggggaaggg cgctgcactt cgtcgacgga cccgacgaag tgcatctgcg
     1021 cacggtggcg cgcgcggagc ttgggaaggc gaaggcgagc cgcggctcga ctgcgccgta
     1081 ctaccggctg ggctgaagtt cggacggtcg cgccgatgcc atcgtggcag gacgccgcgt
     1141 cgccgatgcg ccgcgaggcg cacttcggcg accgcgtcgt gcgctgcttc gccgagcggc
     1201 cgcgcgatct caacgcgatg ttcgcgcagg cagtcgccct ctatggcgcc ggcgaggcgc
     1261 tgatcgacgg cgaccgaagg ctgacctgga gcgcgctcga cgacgaggtc gcgcgcgtgg
     1321 ccgccgggct cgcgcagcgc ggtgtcgaac gcggcgagcg cctggcgctg ctcctcggca
     1381 accgcgccga attcgtgatc gccacgctgg ctgcagcgcg actcggcgcg atcgtggtgc
     1441 cgctgtcgat ccgcgcgcag gcgccggagc tcgcctacat gctcgagcac agcggcgcgc
     1501 gcctcgtcgt gcacgaggcg ggactcgcga
//
LOCUS       DBNHBY010000615         2615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1668026, whole genome shotgun sequence.
ACCESSION   DBNHBY010000615 DBNHBY010000000
VERSION     DBNHBY010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1668026"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..446)
                     /locus_tag="AC16HB_14840"
     CDS             complement(18..446)
                     /locus_tag="AC16HB_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5608112.1"
                     /translation="MNSCELCREVGGEPVLRTDDLRVVLVDEADYPGFVRVIWNAHVR
                     EVTDLTPPQIARLMSAVFAVEQSLRHVMNPLKVNVASLGNVTPHVHWHVIPRFADDPH
                     FPRPIWGERQRQVSAAALDARRALLPTLSTEIRTRVGAMT"
     gene            complement(491..1564)
                     /locus_tag="AC16HB_14845"
     CDS             complement(491..1564)
                     /locus_tag="AC16HB_14845"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5608113.1"
                     /translation="MRLTTQQVFGPGDGLQQPIDLQAPANDARLFIAERTGRLRVVVN
                     GVLRATPFLDISARVSTSGEAGLLSFAFHPQYAAGQPYVFVHFVETGSGDIVVERYRV
                     SATDPNALQTPGTLVVRIAHPGFSNHYGGRVAFGPDGMLYLSTGDGGGGGDPNGNGQN
                     PATLLGKLLRLDVATLPYAVPADNPTWPQAGARRENWAIGLRNPWRYAFDSATGQLYI
                     ADVGQGRIEEIDVVAAGAGGLNYGWNTLEGSQCYPSGDTCSRAGITLPVAEYDHSQGC
                     SITGGYVYRGAALPELAGRYFYSDYCSGWVRSLFYSGGTVSEKTEWIATIGANVAVSF
                     GVDASGELYLLAQDGRVLKLVRG"
     gene            complement(1851..>2615)
                     /locus_tag="AC16HB_14850"
     CDS             complement(1851..>2615)
                     /locus_tag="AC16HB_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HIR5608114.1"
                     /translation="ERLFSQVGLATQAMLWHVGVQCVLPPGYLCCGYPQRGSGQFDRA
                     EKIITDNRVLFHRVANTLNYLDIKTVVVSCGTCYDQLAGYKFEEIFPGCRIVDIHEYL
                     LEKGVKLDGVTGVRYMYHDPCHSPLKQQDPLATVNALIETADHVRIEKNDRCCGESGS
                     LALARPDISTQVRFRKEEEMRKGADKLRTGDFTGAVKILTSCPSCLQGLKRYDDDANT
                     DADYIVIELARHLLGENWLPDYVARANAGGIERVLV"
BASE COUNT          421 a          883 c          886 g          425 t
ORIGIN      
        1 cgaatagctg acggcgccta cgtcatggcg ccgacgcgcg tcctgatctc ggtcgaaagc
       61 gttggcagca gcgcgcgccg cgcatcgagc gcggcggcac tgacctggcg ctggcgctcg
      121 ccccagatcg gtctcgggaa atgcggatcg tcggcgaagc gcggtatcac gtgccagtgc
      181 acgtgcggcg tcacgttgcc gaggctggcg acattgactt tcaacggatt catcacgtga
      241 cgcagcgact gctcgaccgc gaacaccgcg ctcatcaggc gcgcgatctg cggcggtgtc
      301 aggtcggtca cctcacgcac gtgcgcattc cagatcacgc gcacgaagcc gggatagtcc
      361 gcctcgtcca ccaggaccac gcgcagatcg tcggtacgaa gcaccggttc gccgccgact
      421 tcgcggcaca gctcgcatga gttcatatgg cgcctgctcg ggccttggtc gttgcccgga
      481 gtgtacgaag ctagccgcgg accagtttca gcacgcgccc gtcctgcgcc agcagataga
      541 gttcgccgct cgcgtcgacg ccgaacgaca ccgcgacgtt ggcaccgatc gtggcgatcc
      601 attcggtctt ctcgctgacc gttccaccgc tgtagaacag gctgcgtacc cagccgctgc
      661 agtagtccga gtagaagtag cggccggcga gttcaggcag cgcggcgccg cgatagacgt
      721 agccgcccgt gatcgagcag ccctggctgt ggtcatattc ggcaaccggc aatgtgatgc
      781 cggcgcgtga acaggtgtcg ccgctcggat agcactgcga accttcgagc gtattccagc
      841 cgtagttgag cccgcccgcg ccggccgcga cgacatcgat ctcctcgatg cggccctggc
      901 ccacgtcggc gatgtagagc tggccggtgg cggaatcgaa cgcgtagcgc cacggattgc
      961 gcagcccgat ggcccagttc tcacgccgcg cgccggcctg cggccaggtc gggttgtcgg
     1021 ccggtaccgc atacggcaat gtcgcgacgt cgagtcgcag cagcttgccg agcagcgtgg
     1081 ctgggttctg tccgttgccg ttcggatcgc cgccgccgcc gccgtcgccg gtcgacaggt
     1141 acagcatgcc gtcgggcccg aaggcgacgc ggccgccgta gtgattgctg aagcccgggt
     1201 gcgcgatgcg gactaccagt gtgcccggtg tctgcagcgc attgggatcg gtcgccgaaa
     1261 cgcgatagcg ctcgaccacg atgtcgccgc tgccggtctc gacgaaatga acgaacacgt
     1321 acggctgccc ggcggcgtac tgcgggtgaa acgcgaacga cagcagcccg gcttcgccgc
     1381 tcgtcgacac gcgcgcgctg atgtcgagga acggcgtggc gcgcagtacc ccatttacga
     1441 ccacgcgcag ccgtcccgtt cgctccgcga tgaacagtcg cgcatcgttg gccggcgcct
     1501 gcagatcgat cggctgctgc agtccgtcgc ccggaccgaa gacctgttgc gtcgtcagcc
     1561 gcaacgcggc gggcggtggt ggcggcggag gcggcggagg cggcggaggc ggcggtggag
     1621 caggtgcggg cgcaggcgcg ggcgtcgtgc cggcatcgcc gccgccgcaa ccggggaccg
     1681 gcaacgccag agtcagagcc gcggcgaata gcgcaaaccg acgctgcatc ggagcctccc
     1741 cggcccgtgc aggggtgcgg accgcgcaac gatgctagcc cgcgccggcg ccggtcacgc
     1801 cgccatgcag cgcggattcc tgctagcccg aagatccatc gcgcgcggtg ctagaccagt
     1861 acgcgctcga tcccgcccgc gttggctctg gcgacgtaat cgggaagcca gttctcgccg
     1921 agcaggtggc gcgccaactc gataacgatg taatcggcat ccgtgttcgc gtcgtcgtcg
     1981 tagcgcttca gtccctgcag gcacgacggg caggaggtca ggatcttcac cgcgccggtg
     2041 aaatcgccgg tgcgcagctt gtccgccccc ttgcgcatct cctcctcctt gcggaagcgc
     2101 acctgggtcg agatgtccgg ccgcgccagt gcgagcgaac ccgactcgcc gcagcagcgg
     2161 tcgttcttct cgatccgcac atggtcggcg gtttcgatca gcgcgttgac cgtcgcgagc
     2221 gggtcctgct gcttcagcgg cgagtggcag gggtcgtgat acatgtagcg cacgccggtc
     2281 acgccgtcga gctttacgcc cttctcgagc aggtattcgt ggatgtcgac gatgcggcag
     2341 ccgggaaaga tctcctcgaa tttgtagccg gcaagctggt cgtagcaggt gccacagctc
     2401 accaccaccg tcttgatatc gaggtagttg agcgtgttcg cgacacggtg gaacagcacg
     2461 cggttgtcgg tgatgatctt ctccgcgcgg tcgaactgcc cgctgccgcg ctgcggatag
     2521 ccgcagcaca ggtaccccgg cggcagcacg cactgcacgc cgacgtgcca cagcatcgcc
     2581 tgcgtggcga gccccacctg cgagaacagc cgctc
//
LOCUS       DBNHBY010000616         2785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_719936, whole genome shotgun sequence.
ACCESSION   DBNHBY010000616 DBNHBY010000000
VERSION     DBNHBY010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_719936"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="AC16HB_14855"
     CDS             complement(<1..854)
                     /locus_tag="AC16HB_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906738.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5608115.1"
                     /translation="MVWFLAHNTTENMRVRGIQSGFDFLRGAAGFDIGESLIQYDALD
                     PYWKAFLVGVLNTLRVAIVGIVLCTILGALLGIGRFSRNAIVRGLCYGYVELFRNVPV
                     LLQLLMWYLLFTEMLPAPDEPVSFLGLFFLSKGGFAFPVPVWGRGQLLALAGALVGVA
                     VAIYYRRYAMRRFEETGNVPILFWPTVATILVFSLAGWLIGGAPTALNIPGKGEIAVE
                     GGGTVTPEFMAVLLGLVLYTSAFVAEVVRAGIASVAHGQVEAASSLGLSRGHSMRLVI
                     LPQALRVI"
     gene            complement(1036..2070)
                     /locus_tag="AC16HB_14860"
     CDS             complement(1036..2070)
                     /locus_tag="AC16HB_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5608116.1"
                     /translation="MRKTSTKALLGAATLAVAAIATPAHAGKTLDAIKARGQVVCGVN
                     TGLAGFSQADSQGNWSGLDVDICRAIAAAVLGDGSKVKWVPLNAQQRFTALQSGEVDI
                     LSRNTTWTLTRDASLGMHFTNTTYYDGQGFMVPTKGKIKSAKQLKGQTVCVQSGTTTE
                     KNLTDFSRANKLDIKPVVFEKLEAATGAYFSGRCAAYTTDASGLASTRNKEAKNPADH
                     VILPELISKEPLGPAVRRGDDEWFAIAKWVVFGLIEAEEYGITQANVDKLLAESSDPV
                     VLRILGKSEDTGKLLGLDKNWMVNALKAVGNYGEIFERNVGPKTALNLPRGVNNLWSK
                     GGIMYAPPVR"
     gene            complement(2205..>2785)
                     /locus_tag="AC16HB_14865"
     CDS             complement(2205..>2785)
                     /locus_tag="AC16HB_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5608117.1"
                     /translation="RILIDGQDIRDVSQQSLRAVIGIVPQDTVLFNDTIEYNIAYGRP
                     GAARDEVIAAARSAHIHDFIAKLPDGYESMVGERGLKLSGGEKQRVAIARTLLKDPAI
                     LVFDEATSALDSKTERLIQAELEEIARDRTTLVIAHRLSTIVNADQILVLDHGRIVER
                     GTHAQLLAAGGAYARMWRLQQQDAVSRTATAA"
BASE COUNT          460 a          945 c          905 g          475 t
ORIGIN      
        1 atgatcacgc gcagcgcctg cggcaggatc accagccgca tcgaatgccc gcgcgacagc
       61 ccgagcgaac tggcggcctc gacttgaccg tgcgccactg acgcgatgcc ggcgcgcacg
      121 acttcagcca cgaacgccga cgtgtacagc accagcccga gcagcacggc catgaactcg
      181 ggggtcacgg tgccgccgcc ttcgaccgcg atctcgccct tgcccgggat gttcaacgcg
      241 gtgggcgcac caccgatcag ccagcccgcg agcgagaaga ccagaatcgt cgccaccgtc
      301 ggccagaaca ggatcggcac gttgccggtt tcttcgaagc gccgcatggc gtagcgccgg
      361 tagtagatcg ccactgcgac gccgaccagc gcgccggcaa gggccagcaa ttgcccgcgc
      421 ccccacaccg gcaccggaaa cgcaaagccg cccttgctga ggaagaacag cccgaggaac
      481 gacaccggct catccggcgc cggcagcatc tcggtgaaaa gcagatacca catcagcagt
      541 tgcagcagca ccggcacgtt gcggaacagc tcgacgtagc cgtagcacag cccgcgcacg
      601 atcgcgttgc gcgagaagcg gccgatgccg agcagcgcgc cgagaatcgt gcacagcacg
      661 atgccgacga tcgccacccg cagcgtgttg agcacaccaa cgagaaacgc cttccagtac
      721 gggtcgagcg cgtcgtactg gatcagcgat tcgccgatgt cgaacccggc cgcgccgcgc
      781 agaaaatcga agccgctctg gatgccgcgc acacgcatgt tctcggtcgt gttgtgcgcg
      841 aggaaccaga ccaacgcgac gatcaatccg atcgcgatga tctgatagat gacgccgcga
      901 aaggcccggc tgcgccacga ccagtcgcgc cgcttgggcg gaacccgctc cggcagcgcc
      961 tgcgacgttt gatgtcgacg tttcagcgcc cggcgccggg cgttgttctg ccggcaccgg
     1021 acatgccagc ttcagtcagc gcaccggcgg cgcatacatg atgccgccct tgctccacag
     1081 gttgttcacg ccgcgcggca ggttcagcgc ggtcttcggg ccgacgttgc gctcgaagat
     1141 ctcaccgtag ttgccgaccg ctttcagcgc gttgaccatc cagttcttgt ccaggcccag
     1201 cagcttgccg gtgtcctcgc tcttgcccag gatgcgcaga acgaccgggt ccgagctttc
     1261 cgccagcaac ttgtcgacgt tggcctgcgt gattccgtac tcctcggcct cgatcaggcc
     1321 gaacacgacc cacttggcga tcgcgaacca ctcgtcgtcg ccgcggcgca cggcgggtcc
     1381 gagcggctcc ttggagatca gttcgggcag gatcacgtgg tcggccgggt tcttggcctc
     1441 cttgttgcgc gtcgatgcca ggcccgaggc gtcggtggtg taggcggcgc agcggccgga
     1501 gaaatacgcg ccggtggcgg cctcgagttt ctcgaacacg accggcttga tgtcgagctt
     1561 gttggcacgc gaaaagtcgg tcaggttctt ctcggtcgtc gtgcccgact gcacgcacac
     1621 cgtctggccc ttcagctgct tggcgctctt gatcttgcct ttggtcggca ccatgaagcc
     1681 ctggccgtcg tagtaggtcg tgttggtgaa gtgcatgccg agcgaggcat cgcgggtcag
     1741 cgtccaggtc gtgttgcgcg acaggatgtc gacctcaccc gactgcagcg cggtgaaacg
     1801 ctgctgcgcg ttcagtggca cccatttgac cttgctgccg tcgcccagca cggccgccgc
     1861 gatcgcgcgg cagatgtcga cgtccagccc ggaccagttg ccctggctgt cggcctgcga
     1921 gaagccggcc aggccggtgt tgacgccgca aacgacttgt ccgcgcgcct tgatagcgtc
     1981 cagggtcttt ccggcatgcg cgggcgtcgc aatggcagcg accgccagcg tcgcggcgcc
     2041 gagcagcgcc ttggtcgatg tcttcctcat ctgctctatc tccttgttgg atgtttggcc
     2101 ggttgcgccg gagccgcgcg ttcccgcgtc ggggtcaggc aatctaccac cgccgccgag
     2161 aagccagcaa tcggcatcac cctcggggaa gtgcggagcg agtttcaggc cgccgtggcg
     2221 gtacggctca cggcgtcctg ttgctgcaga cgccacatgc gcgcgtacgc gccgccggcc
     2281 gcaagcagct gtgcgtgggt gccgcgctcg acgatgcggc cgtgatccag caccaggatc
     2341 tggtcggcgt tgacgatcgt cgacaggcgg tgcgcgatca ccaaagtggt gcgatcgcgc
     2401 gcgatctcct ccagttcggc ctggatcagg cgctcggtct tgctgtcgag cgccgaggtc
     2461 gcctcgtcga acaccaggat ggcggggtcc ttgagcagcg tgcgcgcgat ggcgacgcgc
     2521 tgcttttcac cgccggagag cttcaacccc cgctcgccga ccatcgactc gtagccgtcc
     2581 ggaagcttcg cgatgaagtc gtgaatgtgc gcgcttctgg cggccgcgat cacctcgtcg
     2641 cgcgcagcgc cgggacggcc gtacgcgatg ttgtattcga tcgtgtcatt gaacagcacc
     2701 gtgtcctgcg gcacgatgcc gatcacggcg cggagcgact gctgggacac gtcgcggatg
     2761 tcctgcccgt cgatcaggat gcgtc
//
LOCUS       DBNHBY010000617         1676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1668276, whole genome shotgun sequence.
ACCESSION   DBNHBY010000617 DBNHBY010000000
VERSION     DBNHBY010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1668276"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="AC16HB_14870"
     CDS             complement(<1..677)
                     /locus_tag="AC16HB_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR5608118.1"
                     /translation="MLYPELFKSLEAVRWNMETDVPWSRFDASKLSEEQAQTIRMNAI
                     TEWSALPATEMFLRDNRHDSDFSAFMSVWFFEEQKHALVLMEYLRRFRPDLVPTHDEL
                     HNVRFEFDPAPALETLTLHFCGEIRLNHWYRRAAEWHTEPVIRHIYKLLSQDEARHGG
                     AYLRYMKKYLAELGDSARAAFSKIGVLMASAKRTAKALHPTNLHVSEKHFPRDTVQSR
                     LPDPQWL"
     gene            complement(702..1127)
                     /locus_tag="AC16HB_14875"
     CDS             complement(702..1127)
                     /locus_tag="AC16HB_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5608119.1"
                     /translation="MSEALARVRRYFETLDESSLARLDTVYAPGAYFKDPFNEVRGVE
                     AIHRIFARMFVQVDAPRFVILEVIGDERQAVLTWEFHFRLRRFAPEPQVIRGASHLRL
                     AADGRIEFHRDYWDAAEELYEKLPLLGALMRWLKRRAAH"
     gene            complement(1124..>1676)
                     /locus_tag="AC16HB_14880"
     CDS             complement(1124..>1676)
                     /locus_tag="AC16HB_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608120.1"
                     /translation="AASAIAERWGGYDLALIVAGTHVEMRAQSFDLDAAKRLLDVNLI
                     GVLNCLDAIVPTLLAQGAGGVGIVSSVAGYLGLPKSLIYGASKAALINLAESLYGDLH
                     PLGIGVYLINPGFVDTPLTRRNDFAMPALMSPGSAARATLDGIAAGRFEIHYPKRFTR
                     WLKLLRVLPYPLQFAAVRRATGL"
BASE COUNT          284 a          562 c          559 g          271 t
ORIGIN      
        1 tcgagccact gcggatccgg cagccgcgac tgcacggtgt cgcgcgggaa gtgcttctcg
       61 ctcacgtgca ggttggtggg gtgcagcgcc ttggcggtgc gcttggcact ggccatcagc
      121 acgccgatct tggagaacgc cgcgcgcgcg ctgtcgccga gctccgcgag gtacttcttc
      181 atatagcgca ggtacgcgcc gccgtggcgc gcctcgtcct gcgacaacag cttgtagatg
      241 tgcctgatca ccggctcggt gtgccattcg gcggcgcgac ggtaccagtg gttcaggcga
      301 atctcgccgc agaagtgcag cgtcagcgtt tccagcgccg gtgccgggtc gaactcgaag
      361 cgcacgttgt gcagttcgtc gtgcgtcggc accaggtcgg gtcggaagcg ccgcaggtac
      421 tccatcagca ccagggcgtg cttctgctcc tcgaagaacc acacgctcat gaacgccgaa
      481 aagtcggagt cgtgccggtt gtcgcgcagg aacatctcgg tggccggcag cgccgaccat
      541 tcggtgatcg cgttcatgcg gatcgtctgc gcctgctctt ccgacagctt cgacgcgtcg
      601 aaccgcgacc acggaacatc cgtttccatg ttccagcgca ccgcttcgag cgacttgaac
      661 agttcgggat acagcatggc catcccctcc atcgaagagc ttcaatgcgc ggcgcggcgc
      721 ttcaaccagc gcatcagggc gcccagcagc ggaagtttct catacagctc ctcggccgcg
      781 tcccagtagt cgcgatgaaa ttcgatccgc ccgtcggccg cgagccgcag gtggctggcg
      841 ccgcggatga cctgcggctc aggggcaaag cggcgcaggc ggaagtggaa ttcccaggtc
      901 agcaccgcct ggcgctcatc gccgatcacc tccaggatca cgaagcgcgg cgcgtcgacc
      961 tgcacgaaca tccgcgcgaa tatgcggtgg atcgcttcca ctccgcgcac ctcgttgaac
     1021 gggtccttga agtacgcgcc gggcgcatac acggtgtcca ggcgtgcgag gctcgactcg
     1081 tcgagcgtct cgaagtagcg ccgcacgcgc gcgagcgctt cgctcatagg ccggtggcgc
     1141 gccgcacggc ggcgaactgc agcggatacg gcagcacgcg caagagcttc agccagcgcg
     1201 tgaagcgctt cggatagtgg atctcgaagc gcccggcggc gatgccgtcg agcgtggcgc
     1261 gcgcggcgga gccgggcgac atcagcgcgg gcatcgcgaa gtcgttcctg cgcgtcagcg
     1321 gcgtgtcgac gaagcccggg ttgatcaggt acaccccgat gccgagcgga tgcaggtcgc
     1381 cgtacagcga ctcggccaga ttgatcagcg ccgccttgct ggcgccgtag atcagcgact
     1441 tcggcaaacc caggtagccg gcgaccgacg acacgatgcc cacgccgccc gcgccctgcg
     1501 ccagcagcgt cgggacgatc gcgtcgaggc agttcagcac gccgatcagg ttcacgtcga
     1561 gcaatcgctt cgcggcgtcg agatcgaacg attgcgcgcg catctcgaca tgcgtgccgg
     1621 cgacgatcag cgccaggtcg taaccgcccc agcgctcggc gatcgcggat gccgcg
//
LOCUS       DBNHBY010000618         4866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_381970, whole genome shotgun sequence.
ACCESSION   DBNHBY010000618 DBNHBY010000000
VERSION     DBNHBY010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_381970"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="AC16HB_14885"
     CDS             <1..1335
                     /locus_tag="AC16HB_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5608121.1"
                     /translation="LSERFDTIVIVRATTRLSHTRSPVQVGNRNERPVRGFVENTAKN
                     VMIPAHARGRHAAIIEREARLRQYLNDAPINRWERGDPRIGVITAGTCYPYVREVLPQ
                     ASVLKLGASWPLPERLLRDFCASVERVFVVEELEPFIERELRTLGISASGKDFFPRTG
                     EFAPEVVRAGFEKAGVLPVRPRRSAAWAPQPIARPPVLCAGCPHTSTYMAVRAMGARV
                     AGDIGCYTLAALDPLRGIDTSVAMGCSIGVAAGMARGGETRPVLATIGDSTFLHAGIP
                     ALIDAVYNDANITVILLDNHITAMTGGQDHPGTGRNLRGEPAPHVDYEALVRAIGVKW
                     VRRIDSYDLADMYQTLREAIMHRGVSVVISDRPCVIDPVKIKGPPLAIVEPLCNACQS
                     CMNLGCPALTWDDATFEGRHKVKIEPTLCIGCTLCAQVCTVDCIKPPAVALQ"
     gene            1344..2258
                     /locus_tag="AC16HB_14890"
     CDS             1344..2258
                     /locus_tag="AC16HB_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5608122.1"
                     /translation="MQAKGEPHAASVAPVNVLIVGVGGQGVILVSKVLAYLAQSRGLQ
                     VKQSEVHGMAKRGGTVFSHVRFGPQVWSPTIPRGEADILVALEWAEGMRWLPWLKPDA
                     GIFVCDTKRIVPPFACLARQPGATLRYSRETPADVREHVAEGYAIDATHIAAELGNER
                     AANVVLLGALSTALEFPVADWEAALTEFVPQKTVGINLKAFRRGREWIERARSCVAPD
                     DTAAAVAHVDATAPYEVRLEITPQWCKSCDICVKLCPERCLRLNDERIVELTDPALCT
                     GCRLCEWLCPDFAIRVHLDPVAPAGAAA"
     gene            2255..3436
                     /locus_tag="AC16HB_14895"
     CDS             2255..3436
                     /locus_tag="AC16HB_14895"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5608123.1"
                     /translation="MNAVAPNLARPDSVRPTVRLISGNHACALAAVAAGCRFFAGYPI
                     TPSSEVAERLSRLLPPVDGVFVQMEDEIASMAAVIGASMGGVKAITATSGPGFSLKLE
                     NLGYAAAAEIPCVVVDVMRGGPSTGMPTRPAQADLMQTRWGSHGDYPIIVLAPASVRE
                     VFDQTVRAFALSESLRTPVVVLYDQSIAQLTETVELPAPETIVQVERRWAHGPREGFQ
                     PYAAGDDGVPAMARPGDGYRTHTTGLTHSESGFPSQKPEVVERNQTRLLTKLQRNRAR
                     IDAFETIGCDDAEVLLVAIGISARAAKRAAALARERGVKAGLFRPITLWPFPEDALRA
                     AAARARAVLVPEMNAGQLRLEVERILRAIPVAGLNRYDGEPITPAAIAARAETLARET
                     K"
     gene            3436..4284
                     /locus_tag="AC16HB_14900"
     CDS             3436..4284
                     /locus_tag="AC16HB_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5608124.1"
                     /translation="MLESAADFDVRAYLRLDMMPHFLCPGCGHGIALRALLWALDEIR
                     IDRDQLAVISGIGCAGRLSAYVEASTYHVTHGRPLAYATGLALARPDLRVVVITGDGD
                     ALAIGGNHLIHACRRNLKMTCLMLNNEIYGMTGGQVSPTTSESMLTTTTPLGNAEPAF
                     DACSLAAAAGAGFVGREVTHHVPKLKELIRQGLEYPGFAFIEVLSDCTEIFGRKNELG
                     SSPEMILRQKSEIRPSAYRDTVDAPFRSTDLRTGVLSTNDRAEYGAAYRRAAAAAKAA
                     AATAAK"
     gene            4281..4835
                     /locus_tag="AC16HB_14905"
     CDS             4281..4835
                     /locus_tag="AC16HB_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5608125.1"
                     /translation="MKNGHRLELRIAGTGGQGLILAGRMLAEALVASGRRVAQSQTYE
                     PTSRGGYCNSDLVIDDTEVDYPLATALDALVLLDRLATAPSWPLLKPGALVLADARLC
                     PALPDGQVRALHLPLTRTAIELGSERVANIVSLGALAALAGICDRETLERAVLADTPR
                     SFVQLNVEALAAGYRLAAQATAAA"
BASE COUNT          725 a         1652 c         1772 g          717 t
ORIGIN      
        1 ctgtccgagc gcttcgacac catcgtgatc gtgcgcgcga cgacgcggct gtcgcacacg
       61 cgcagcccgg tgcaggtcgg caaccgcaac gagcggccgg tgcgcggctt cgtcgagaac
      121 accgccaaga acgtgatgat tccggcgcac gcgcgcggcc gtcacgcggc gatcatcgag
      181 cgcgaggcca ggctgcggca gtacctgaac gacgcaccga tcaaccgctg ggagaggggt
      241 gacccgcgca tcggtgtcat caccgcgggt acctgctatc cgtacgtgcg cgaggtgctg
      301 ccgcaggcga gcgtgctgaa gctgggcgcc tcgtggccgc tgccggagcg gctgctgcgc
      361 gatttctgcg cgtccgtcga acgcgtgttc gtggtggagg aactggaacc tttcatcgaa
      421 cgcgaactgc gcacgctcgg aatctccgcg tccggcaagg atttctttcc ccgcaccggc
      481 gagttcgcgc cggaggtggt gcgcgccggc ttcgagaagg cgggcgtgct gccggtgcgg
      541 ccgcgacgat ccgccgcgtg ggcgccgcag ccgatcgcgc gcccgccggt gctgtgcgca
      601 ggctgcccgc acacgtcgac ctacatggcg gtgcgtgcga tgggcgcgcg cgtggcgggc
      661 gacatcggct gctacacgct ggccgcgctc gatccgctgc gcggcatcga cacgtcggtc
      721 gcgatgggct gctcgatcgg ggtggccgcg ggcatggctc ggggtgggga gacgcgcccc
      781 gtgctggcga cgatcggcga ttcgaccttc ctgcacgcgg gaatcccggc gctgatcgac
      841 gccgtctaca acgacgccaa catcacggtg atcctgctcg acaaccacat caccgcgatg
      901 accggcgggc aggaccaccc gggcaccggc aggaacctgc gcggcgagcc ggcgccgcac
      961 gtcgattacg aggcgctggt gcgtgcgatc ggcgtcaagt gggtgcgccg gatcgactcc
     1021 tacgatctcg ccgacatgta ccagacgctg cgcgaggcga tcatgcatcg cggcgtgtcg
     1081 gtcgtgatct ccgaccggcc gtgcgtgatc gacccggtga agatcaaggg cccgccgctg
     1141 gcgatcgtcg agccgctgtg caacgcgtgc cagtcctgca tgaacctcgg ctgcccggcg
     1201 ctgacctggg acgacgcgac cttcgagggc cgacacaagg tgaagatcga gccaacgctg
     1261 tgcatcggct gcacgctgtg cgcgcaggtg tgcacggtcg actgcatcaa gccgccggcg
     1321 gtggcgctgc aatgagcggg agcatgcagg cgaaaggaga gccgcacgcg gctagcgtcg
     1381 cgcccgtgaa cgtgctgatc gtgggcgtgg gcggccaggg tgtgatcctg gtgtcgaagg
     1441 tgctcgcgta cctcgcgcag tcgcgcgggc tgcaggtcaa gcagagcgag gtgcacggca
     1501 tggccaagcg cggtggcacc gtgttcagcc acgtgcgctt cggtccgcag gtgtggtcgc
     1561 cgacgattcc gcgcggcgag gccgacatcc tggtcgcgct ggagtgggcc gaggggatgc
     1621 gctggctgcc gtggctcaaa cccgacgccg gcatcttcgt gtgcgacacc aagcgcatcg
     1681 tgccgccgtt cgcgtgcctc gcccgccagc ccggcgcgac gctgcgctac tcgcgcgaaa
     1741 cgccggccga cgtgcgcgag cacgtggccg agggctatgc gatcgacgcc acccacatcg
     1801 ccgcggaact cggcaacgag cgcgcggcga acgtcgtgct gctcggcgcg ctgtcgacgg
     1861 cgctcgagtt cccggtcgcg gactgggaag cggcgctgac cgagttcgtg ccgcagaaga
     1921 cggtcggtat caacctgaag gcgtttcgcc gcggccgcga atggatcgag cgtgcgcgca
     1981 gctgtgtggc gcccgacgac acggcggccg cggtcgccca cgttgatgca accgcgccgt
     2041 acgaggtgcg gctggagatc acgccgcagt ggtgcaagag ctgcgacatc tgcgtgaagc
     2101 tgtgcccgga acgctgcctg cggctgaacg acgagcgcat cgtcgagctg accgacccgg
     2161 cgctgtgcac aggctgtcgg ctctgcgagt ggctgtgccc ggacttcgcg atccgtgtgc
     2221 atctcgatcc ggttgcgccc gcaggagccg ccgcatgaac gccgtcgccc cgaacctcgc
     2281 gcgccctgac tctgtccgtc cgacggtgcg gctgatctcc ggcaaccacg cgtgcgcgct
     2341 tgcggcggtg gcggccggct gccgtttctt cgccggctat ccgatcacgc cgtcctcgga
     2401 agtggccgag cgtttgtcgc ggctgctgcc gccggtcgat ggcgtcttcg tgcagatgga
     2461 agacgagatc gcatcgatgg ccgcggtgat cggcgcttcg atgggcggcg tcaaggcgat
     2521 aactgcgacc agcggccccg gcttttcact gaaactggaa aacctcggtt atgcggcggc
     2581 ggcggagatt ccgtgcgtgg tggtcgacgt gatgcgcggc ggcccgtcga ccggcatgcc
     2641 gacgcgtccg gcgcaggccg acctgatgca gacgcgctgg ggcagtcacg gcgactatcc
     2701 gatcatcgtg ctggcgccgg cgtcggtgcg cgaggtcttc gaccagaccg tgcgcgcatt
     2761 tgcgttgagc gaatcgctgc gcacgccggt cgtggtgctg tacgaccagt cgatcgcgca
     2821 actgaccgag accgtcgaac tgccggcacc ggagacgatc gtgcaggtgg agcgccgctg
     2881 ggcgcacggc ccgcgcgagg gtttccagcc ctacgcggcc ggcgacgacg gcgtgccggc
     2941 gatggcgcgg ccgggcgacg gctatcgcac gcacacgacc gggctcacgc actcggagag
     3001 cggatttccg agccagaagc ccgaggtggt cgagcgcaac cagacgcggc tgctgaccaa
     3061 gctgcagagg aaccgcgcgc gcatcgacgc gttcgagacg atcggttgcg acgacgccga
     3121 ggtgttgctc gtggcgatcg gcatcagcgc gcgcgcagcg aagcgcgcgg cggcgttggc
     3181 gcgcgagcgt ggcgtcaagg cgggcctgtt ccgtccgatc acgctgtggc cgttcccgga
     3241 ggacgcgctg cgcgccgcgg cagcacgcgc acgcgcggtg ctggtgccgg agatgaacgc
     3301 gggccaactg cggctggagg tggagcgcat cctgcgcgcg ataccggtcg cgggcctgaa
     3361 ccgctacgac ggcgaaccga tcacgccggc agcgatcgct gcgcgcgccg agacgctcgc
     3421 gagggagacg aagtgatgct cgaaagcgcc gcggatttcg acgtccgcgc ctacctgcgg
     3481 ctggacatga tgccgcactt cctgtgcccc ggctgcgggc acggcatcgc gctgcgtgcc
     3541 ctgctgtggg cgctggacga gatccggatc gatcgcgacc agctcgcggt gatctccggc
     3601 attggctgcg cgggacgact gtcggcctac gtcgaggcca gcacgtacca cgtcacgcac
     3661 ggccggccgc tggcgtacgc gacgggcctg gcactggcgc ggcccgacct gcgcgtggtc
     3721 gtgatcaccg gcgacggcga cgcactcgcg atcggcggca accacctgat ccacgcgtgc
     3781 cggcgcaacc tgaagatgac ctgcctgatg ctgaacaacg agatctacgg catgaccggc
     3841 gggcaggtgt cgccgaccac gtccgagtcg atgctgacga ccaccacgcc gctcggcaac
     3901 gccgagccgg cgttcgacgc gtgctcgctc gcggcggcgg cgggcgcggg cttcgtcggg
     3961 cgcgaagtca cgcatcacgt gccgaagctc aaggaattga tccggcaggg actcgagtac
     4021 ccgggtttcg ccttcatcga ggtgctgtcg gactgcaccg agatcttcgg ccgcaagaac
     4081 gaactcggct cctcgcccga gatgatcctg cggcagaaga gcgagatccg gccgagcgcg
     4141 tatcgcgaca cggtcgatgc gcccttccgc tcgactgacc tgcgcaccgg cgtgctgtcc
     4201 accaatgacc gcgccgaata cggcgccgca tatcgccgcg ctgcagcggc ggcgaaggcg
     4261 gccgcggcca cggcggccaa gtgaagaacg gtcaccgcct cgaactgcgc atcgccggca
     4321 ccggcgggca ggggctgatc ctggctggca ggatgctggc cgaggcgctc gtcgcgtcgg
     4381 gccggcgcgt cgcgcagtcg cagacctacg agccgacgtc gcgcggcggt tactgcaatt
     4441 cggacctcgt gatcgacgac accgaggtcg actatccgct cgcaaccgcg ctcgatgcgc
     4501 tggtgctgct cgacagactc gcgaccgcgc cgtcgtggcc gctgctcaag cccggcgcgc
     4561 tggtgctggc cgacgcgcgc ctgtgccccg cgctgccgga cggccaagtg cgcgcgctgc
     4621 acctgccgct gacgcgcacc gcgatcgagc tgggcagcga acgcgtggcg aacatcgtgt
     4681 cgctcggcgc gctggccgcg ctggcgggca tctgcgatcg cgagacgctc gagcgcgcgg
     4741 tgctcgcgga tacgccgcgc agcttcgtgc aactgaacgt cgaggcactg gcggccggtt
     4801 accgactcgc tgcgcaggcg accgcggccg catagcgcgg cgctgcactc gcgttcgccg
     4861 cgcgct
//
LOCUS       DBNHBY010000619         2071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1735467, whole genome shotgun sequence.
ACCESSION   DBNHBY010000619 DBNHBY010000000
VERSION     DBNHBY010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1735467"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..313)
                     /locus_tag="AC16HB_14910"
     CDS             complement(77..313)
                     /locus_tag="AC16HB_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HIR5608126.1"
                     /translation="MRVVVQHRGRYHYPRPAPPGPQLIRLRPADRTRARIESYQLLVE
                     PEHRLHWLCDPHGIRVARVTFKAGQAVSECSTSR"
     gene            complement(330..824)
                     /locus_tag="AC16HB_14915"
     CDS             complement(330..824)
                     /locus_tag="AC16HB_14915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5608127.1"
                     /translation="MQRAAAIIGSAIFLVIAPGTLAVYVPWYLTNWHFAPPIFPIARV
                     LGAALIVAGLPILLDSFARFELQGLGTPAPVMPPKRLVVTGFYRYVRNPMYVAVTALI
                     AGQGLLFGSVTVLKYGAILWAGFFLFVVAYEEPVLGEQFADEYKRYRANVRRWLPRIT
                     PWRG"
     gene            complement(864..1853)
                     /locus_tag="AC16HB_14920"
     CDS             complement(864..1853)
                     /locus_tag="AC16HB_14920"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HIR5608128.1"
                     /translation="MHVNRRDVIFAFAVSAALGGCATPRAPAADWQQFSAGDGTAINY
                     RRWVSAGAAPKAVLQIVHGAAEHASRYDRFAQAAVASGYAVYGTDHRGHGRTRLRSGR
                     TGDAGPDAWNRMVEDEIAFTQRLRAAHPGAKLVLFGHSLGSFMAQDYIERRGDLLDAL
                     VLSGTSYRPGVPQQLIDLLNDAVRKEPLGDSAVWAGFFKDYNKPFGGPTGFEWLSRDS
                     SEVKKYVDDPLCGFAFSNELARDIFVGLARMRDPALEARIPTSLPVLVIQGELDPVGE
                     NLVGTRRLVARYQALGMSRIETRYYTGARHELLNETNRDEVQRDVLAWLAKTV"
BASE COUNT          376 a          675 c          706 g          314 t
ORIGIN      
        1 gatcacaaca acccgggcag cactcgcgga atcatcatca tcgggggctg accggcacga
       61 cactgagctt cgggcttcac cgacttgtgg agcactcgga gaccgcttgc ccggccttga
      121 acgtgacgcg cgcgacgcga ataccgtgcg gatcgcacag ccagtgcagg cgatgctccg
      181 gctcgaccag cagctgatag ctctcgatcc gcgcgcgcgt gcgatcggcg ggccgcaacc
      241 ggatcagctg cgggcctggc ggcgccggtc tcggatagtg gtagcggccg cgatgctgga
      301 caacgacgcg cacgcagcgt tcttaccacc tacccgcgcc atggggtgat gcgcggtaac
      361 catcgcctca cgttcgcccg gtagcgcttg tactcgtcgg cgaactgctc gcccagcaca
      421 ggctcttcgt aggcgacaac gaacaagaag aatccggccc acaggatcgc gccgtacttc
      481 agcaccgtga cgctcccgaa caggaggccc tggccggcga tcaacgccgt gactgcgacg
      541 tacatcggat tgcggacgta gcgatagaac ccggtgacga cgaggcgctt gggaggcatc
      601 acgggcgccg gagtgccgag gccctgcaac tcgaagcgag cgaacgaatc gagcaagatg
      661 ggtaacccgg cgacgatcag ggcggcgccg agcacgcgcg cgatcgggaa tatcggaggc
      721 gcgaagtgcc agttcgtgag gtaccaaggg acgtacacgg cgagcgtgcc tggagcgatg
      781 accaggaaga tcgcggaacc gatgatcgcg gcggcgcgct gcacgatggg agcaatgact
      841 cgggatgtgg ctttcaggac cgctcaaacc gtcttcgcca gccacgccag cacgtcgcgc
      901 tggacctcgt cgcggttggt ctcgttcagc aactcgtgcc gcgcacccgt gtagtagcgg
      961 gtctcgatgc gcgacatccc gagtgcctgg tagcgcgcca cgaggcgccg cgtaccgacg
     1021 aggttctcgc ccaccgggtc gagctcgccc tggatcacca gcaccggcaa ggacgtggga
     1081 atacgcgcct cgagtgccgg gtcgcgcatg cgcgcgaggc cgacgaagat gtcgcgcgcc
     1141 agctcgttgc tgaaggcgaa gccgcacagc ggatcgtcga cgtacttctt cacttcggaa
     1201 gaatcgcggc tcagccactc gaagcctgtg gggccgccga agggcttgtt gtagtccttg
     1261 aagaagcccg cccataccgc cgagtcgccg agcggctcct tgcgcaccgc gtcgttgagc
     1321 agatcgatca gctgctgcgg cacgcccgga cggtacgacg tgccgctcag cactagggca
     1381 tcgagcagat cgccgcggcg ctcgatgtag tcctgcgcca tgaacgagcc gaggctgtgg
     1441 ccgaacagca cgagcttggc accgggatgc gcggcgcgca ggcgctgcgt gaaggcgatt
     1501 tcgtcttcga ccatccggtt ccaggcatcg gggccggcat cgccggtgcg gcccgagcgc
     1561 agccgcgtgc ggccgtgacc gcgatggtcg gtgccgtaga ccgcatagcc gctcgcgacg
     1621 gccgcctgcg cgaaacgatc gtagcggctg gcgtgctcgg ctgcgccgtg cacgatctgc
     1681 agcactgcct tcggtgccgc gccggccgat acccaacgcc ggtagttgat agcggtgccg
     1741 tcgccggcgg agaactgctg ccagtcggca gccggcgccc ttggggtggc acagccgccg
     1801 agggcggcgc taacggcaaa ggcgaagatc acgtcgcggc gatttacgtg cacggtcgtc
     1861 tcctcgtctc gttggcgctc ggctcgaggc cggtacggtc cagtgtagga gtgggatcgg
     1921 cgcaagaacc ctgaggcact cgaacttcgt cagcccttgt cgaaccgtgt tccgaatgag
     1981 ccggcccaac aagcggcaaa gcggccgttg gcgaagcacc tagagcaatc ccgcaattcc
     2041 cagccccatc gaagcggcaa atgcggtggt t
//
LOCUS       DBNHBY010000620         1795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_788710, whole genome shotgun sequence.
ACCESSION   DBNHBY010000620 DBNHBY010000000
VERSION     DBNHBY010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_788710"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16HB_14925"
     CDS             <1..340
                     /locus_tag="AC16HB_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5608129.1"
                     /translation="GNPGPGFNVVWENLRLMERIARVNGAAVLPTDFAACNAYAGAFD
                     AARTLICEALFVLGAGDQMTPPKAAQALIDACPRKTVVTLPATGHALMSENPDGVRDA
                     LQKFAARVRG"
     gene            469..1107
                     /locus_tag="AC16HB_14930"
     CDS             469..1107
                     /locus_tag="AC16HB_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5608130.1"
                     /translation="MLDAVNWNTYGLYVLTEAALSLSPGPAVMLVIACGIARGTRKSI
                     FAGLGILSANALYFALSATALGAVLVSSHAFFNAVKWIGAAYLVYIGTSALFGRPSPI
                     AISNGGAGSASRRAIYLSGLTLQLANPKTLIFFIAILPQFVDPHLPIGAQMVWLAAGS
                     IVPEFFILAGYGWLGSRAARLAADPRYARWTERAAGMLVLCAAALVISVGTA"
     gene            complement(1124..>1795)
                     /locus_tag="AC16HB_14935"
     CDS             complement(1124..>1795)
                     /locus_tag="AC16HB_14935"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5608131.1"
                     /translation="PSIGAPAARSVGNPDGSSDTVMFGSNSFPAPNAMTFSRNGSLYV
                     SDSFQGAIYRIDNVATCSKPCAVSLVVHDSLLATAGFPPFGANGVALNADETALFIAN
                     TGDNRVLKLDLATKQVSVFAESVHGADGLTFDGNGRLWVAANQADQLVALSESGRVIA
                     RLGEFQGINKDGSPNGLLFPASPVIVGGWMYVTNLAIPLTGNPAEWEADVKRWTIARI
                     KVPNR"
BASE COUNT          269 a          606 c          607 g          313 t
ORIGIN      
        1 gggcaacccc ggaccgggct tcaacgtcgt gtgggaaaac ctgcggctga tggagcggat
       61 cgcgcgcgtg aacggcgcgg cggtgctgcc gaccgacttt gcagcgtgca acgcgtacgc
      121 gggcgcattc gacgcggcgc gcacgctcat ctgtgaagcg ctgttcgtgc ttggcgctgg
      181 cgaccagatg acgccaccga aagccgcgca ggcattgatc gacgcctgcc cgcgcaagac
      241 tgtcgtcacg cttccggcaa ccggccacgc gctgatgagc gagaaccccg acggcgtgcg
      301 cgacgcgttg cagaagttcg cggcgcgggt ccgcggctga gaaggcgtga agtattcgtc
      361 gcgagcggcc cggggtcgcg ccgtgcagcg gacctgtaac gagtcgtgcg gcgagcaacg
      421 caggcgcgag cgcggcccgc gcagtagaga gtctcacgca ttctgaccgt gctcgacgcg
      481 gtcaactgga acacttacgg gctgtatgtc ctgaccgagg ccgcgctcag cctgtcgccc
      541 ggcccggcgg tgatgctggt catcgcgtgc ggcatcgcgc gcggcacgcg caagtcgatc
      601 ttcgccgggc tcggcatcct gtccgcaaac gcgctgtatt tcgcgctgtc ggcgaccgcg
      661 ctcggcgcgg tgctggtgtc gtcgcatgcg ttcttcaacg cggtcaagtg gatcggcgcg
      721 gcgtacctcg tctacatcgg tacctcggcg ctgttcggcc ggccatcgcc gatcgcgatc
      781 tcgaacggcg gcgccggcag cgcctcgcgc cgcgcgatct acctgtcggg gctgacgctg
      841 caacttgcca atccaaagac gctgatcttc ttcatcgcga tcctgccgca gttcgtcgat
      901 ccgcatctgc cgatcggcgc gcaaatggtc tggctcgccg cgggttcgat cgtccccgag
      961 ttcttcatcc tcgcgggcta cggttggctc ggctcgcgtg cggcacggct cgccgccgat
     1021 ccgcgctacg cgcgctggac cgaacgcgcc gccggcatgc tcgtcctgtg cgcagcggcg
     1081 ctcgtgatct cggtcggtac ggcgtgaaac gacgccgcgt gcgctagcgg ttcggcacct
     1141 tgatgcgggc aatcgtccag cgcttcacgt ccgcttccca ctccgccggg ttgccggtca
     1201 gcggtatggc gagattggtc acatacatcc agccgccgac gatcaccggg ctggccggga
     1261 acagcagccc gttgggcgac ccgtccttgt tgatgccttg gaattcgccg agccgagcga
     1321 tgacgcggcc gctttcgctc aacgcgacca gctgatcggc ctggttggcc gcgacccaga
     1381 gccggccgtt gccgtcgaag gtcaagccgt cggcgccgtg cacgctttcc gcaaacacgg
     1441 agacctgctt cgtcgccaga tcgagcttca gcacgcggtt gtcgccggtg ttggcgatga
     1501 aaagtgccgt ttcgtcggcg ttcagcgcga cgccgttggc accgaacggt ggaaagcccg
     1561 cggtcgccag cagcgaatcg tgcacgacca gcgacaccgc gcacggcttg ctgcatgtcg
     1621 cgacattgtc tatgcggtag atcgcaccct ggaacgaatc cgacacgtac aggctgccgt
     1681 tgcggctgaa cgtcatcgcg ttcggcgccg gaaagctgtt cgaaccgaac atgaccgtgt
     1741 cgctgctgcc gtccggattg ccgacgctgc gcgcagcggg cgcgccgatc gacgg
//
LOCUS       DBNHBY010000621         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_43491, whole genome shotgun sequence.
ACCESSION   DBNHBY010000621 DBNHBY010000000
VERSION     DBNHBY010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_43491"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC16HB_14940"
     CDS             <1..369
                     /locus_tag="AC16HB_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5608132.1"
                     /translation="VRDATGEAGVDRVIEVDFAANVALDLAVLKPEGDLIVYGSGKPE
                     IAVPFFPSIVKNVRVRFFIVYNLTPADRAAAIGQLGAWLEADRLQHNIAARLPLDRIA
                     EAHELVESGRAVGNVVLEID"
     gene            complement(366..1553)
                     /locus_tag="AC16HB_14945"
     CDS             complement(366..1553)
                     /locus_tag="AC16HB_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR5608133.1"
                     /translation="MSFGRRLLACALALWCAGLVLVDARAADPQTIEAFVREGCPHCA
                     EAEAFLDRLRKERPELRIVIRDVQKEATALERLQQIARELGAGDARVPAIHVRGQLIV
                     GYSAGAGTDRLIRAALAGRRDAAPGKTPGDSCAAENELACRPPAADSTEPEPFELSLF
                     GTTLTLDGVGLPVFTLAMGLLDGFNPCSMWVLVLMISLLAPLNDRPRMLAIAGTFVLV
                     DGIAYFLFMTAWLNLFLLIGLSRASQLVVAAIAIVAGLINMKDFVAFGRGISLSIPER
                     AKPGIYSRMRDILHARNLTAAIIGTVILAWLVQIVEFMCTSGFPALYTRILTLRQLDP
                     ASYYGYLLLYNAAYMLDDVVVLGIGVVLLSRHRLQEREGRWLKLISGLVMVGLGVYLL
                     VER"
     gene            1859..2029
                     /locus_tag="AC16HB_14950"
     CDS             1859..2029
                     /locus_tag="AC16HB_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608134.1"
                     /translation="MNTTSKHIRIAGFLFSILMSTTVIGATILSMSPALAVDSAQVIA
                     LEHVTITAPATN"
BASE COUNT          373 a          717 c          690 g          306 t
ORIGIN      
        1 gtgcgcgacg ccaccggtga ggcgggcgtg gatcgcgtga tcgaagtcga cttcgccgcc
       61 aacgtcgcgc tcgacctcgc cgtgctgaaa cccgagggcg acctcattgt ctacggcagc
      121 ggcaagccgg aaatcgcggt gccgttcttc ccgtcgatcg tcaagaatgt ccgcgtgcgg
      181 ttcttcatcg tctacaacct gacgccggcc gaccgcgccg ccgcgatcgg gcagcttggt
      241 gcatggctcg aagccgatcg gctgcagcac aacatcgccg cgcgtctccc actcgatcgc
      301 attgccgagg cgcacgaact ggtcgagagc ggccgcgcgg tcggcaacgt cgtgctcgag
      361 atcgactagc gctcgacgag cagatacacg ccgagcccga ccatcacgag gccgctgatc
      421 agcttcagcc agcggccctc gcgttcctgc agccgatggc ggctgagcag gaccacgccg
      481 atgccgagca cgacgacgtc gtcgagcatg taggcggcgt tgtacaagag caggtagccg
      541 tagtagctcg ccgggtccag ttgccgcagc gtcaggatcc gcgtgtacag cgccgggaag
      601 cccgacgtgc acatgaactc gacgatctgc accagccagg ccaggatcac cgtgccgatg
      661 atcgctgcgg tcaggttgcg cgcgtgcagg atgtcgcgca tgcggctgta gatgcccggc
      721 ttggcccgct cgggtatcga cagcgagatc ccgcgaccga aggcgacgaa gtccttcatg
      781 ttgatcagtc ccgcgacgat cgcgatcgcg gccacgacga gctgcgacgc gcgcgaaagt
      841 ccgatcagca ggaacaggtt cagccatgcc gtcatgaaca ggaagtacgc gatcccatcg
      901 accagcacga acgtgcccgc gatcgcgagc atccgcggcc ggtcattcag cggtgcgagc
      961 agcgagatca tcagcaccag cacccacatc gagcaggggt tgaagccgtc gagcaggccc
     1021 atcgccagcg tgaagaccgg cagaccgacg ccgtcgagcg tgagcgtcgt gccgaagagg
     1081 gacagttcga agggctcggg ttcggtcgag tccgccgccg gtggccggca cgcgagttca
     1141 ttctcggccg cgcaggagtc gcccggcgtc ttcccgggcg cggcatcgcg gcggcccgcg
     1201 agcgccgcgc ggatcagccg gtccgtgccg gcccccgcgg agtagccgac gatcagttgg
     1261 ccgcgcacgt ggatcgcggg cacgcgcgcg tcgccggcgc cgagttcgcg cgcgatctgc
     1321 tgcagtcgct ccagcgccgt ggcttccttc tgcacgtcgc ggatcacgat ccgcagttcc
     1381 ggccgctcct tccgcaggcg atcgaggaac gcctcggctt cggcgcagtg cgggcaacct
     1441 tcgcggacga aggcttcgat ggtctgcggg tcggccgccc gcgcatcgac gagaacgaga
     1501 ccggcgcacc acagcgcgag tgcgcacgca agcaaacggc ggccgaagct caaaggcgct
     1561 ccagggaagg acgcaggcag tctatgaagt cgcagcgacc cgttgttgcg ccaggtccgc
     1621 cacgccagcg cggccggttg ccctggatca agcccggcgg cggctgaaat gccggcaggc
     1681 gcgacgaagc ggcgaaaagc cggaatgaac tgcggaatcg gtcgtcgggg cgacgaaagc
     1741 gcctttgcag ttcgtccccc gttttctgcg gccggtcgca atccgctcgc gcccggtgcc
     1801 gcccgtgcct acagttcgct ccatcgcagc caaccgaccg caatgaaagg accgaaccat
     1861 gaacaccacc agcaaacaca tccgcatcgc cggttttctg ttttcgatct tgatgagcac
     1921 tacggtgatc ggcgccacca ttctgtcgat gtcccccgcg ctggcggtcg atagcgcgca
     1981 ggtcatcgcg ctcgagcacg tgaccatcac cgctcccgcg accaactgaa cggctgcgcc
     2041 gaacggcgcg gcgctcggtc ccgccgcgca gcgaacggac caaagc
//
LOCUS       DBNHBY010000622         2119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_788916, whole genome shotgun sequence.
ACCESSION   DBNHBY010000622 DBNHBY010000000
VERSION     DBNHBY010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_788916"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC16HB_14955"
     CDS             <1..848
                     /locus_tag="AC16HB_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5608135.1"
                     /translation="RTNGRVKVEVYPNSTLYKDNEEIEALQLGTVQMLAPSVSKFGPL
                     GVREYEAFDLPFIFPDRNVLAHVTEGPVGASLFKKLEAKGIRGLAYWDNGFKVMTANR
                     PLRMPEDLKGLKMRIQSSKVLDAQFRAVGAIPQVMAFSEVYQALQTGVVDGTENTPSN
                     VYTQKMHEVQKYLTLSDHGYIGYAVIVNAKFWDGLPADIRTALEDAMRDATRYANAIA
                     RQENDQALAGIKAAGKTEIITLDDRQKAAWRKAMQPVYKDMESRVGKDLIAAIQKEAA
                     AAGYK"
     gene            860..1690
                     /locus_tag="AC16HB_14960"
     CDS             860..1690
                     /locus_tag="AC16HB_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5608136.1"
                     /translation="MTARSYLFVPGDRPDRFAKALASGADAVIVDLEDAVAPEAKADA
                     RAQLAAWLRVATARVIVRINGADTEWFADDLALAALPAVAGVMLPKAERVEDLARVAQ
                     AAPRRTLLPLIETARGFDRAREIAQATSVARLVFGSIDFQHDLNIRGDAEELLYFRSQ
                     LVLVSRLAGIEAPVDGVCTAIDDAQRLEDEALRARRLGFGAKLCIHPKQVGVVNRAFS
                     PSAEEIAWARRVLQAAGEARGAAIAVDGKMVDRPVLLRAEAIVREAARGDAPNDDGRD
                     "
     gene            1674..>2119
                     /locus_tag="AC16HB_14965"
     CDS             1674..>2119
                     /locus_tag="AC16HB_14965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5608137.1"
                     /translation="MTAATDPRADTLRRLQAFWDERYAADEFAYGTAPNDFLVQRLGR
                     IAPRGRVLCLADGEGRNGVWLAQQGLQVTSVDVSARGLDKAQALARQAGVHIDAVVCD
                     VTRFDVGTAAWDAITSIFLHLPAKPRRALHERCAAALKPGGVFIYEA"
BASE COUNT          351 a          663 c          791 g          314 t
ORIGIN      
        1 aacgcacgaa cgggcgcgtg aaggtcgagg tctatccgaa ctcgacgctg tacaaggaca
       61 acgaggagat cgaggcgctg cagctcggca cggtgcagat gctggcgccg tcggtgtcga
      121 agttcggtcc gctcggcgtg cgcgagtacg aggcattcga tctgccgttc atctttcccg
      181 atcgcaacgt gctggcgcac gtgaccgaag gcccggtcgg cgcatcgctg ttcaagaagc
      241 tggaagcgaa gggcattcgg ggactcgcat actgggacaa cggattcaag gtgatgacgg
      301 ccaaccgtcc gctgcgcatg ccggaggacc tgaagggtct gaaaatgcgc atccagtcgt
      361 ccaaggtgct cgacgcgcag ttccgcgcgg tcggcgcgat cccgcaggtg atggcgttct
      421 ccgaggtcta ccaggcgctg cagacgggcg tggtcgacgg caccgagaac acgccgtcga
      481 acgtctatac gcagaagatg cacgaggtgc agaagtacct gacgctgtcg gaccacggct
      541 atatcggcta cgcggtgatc gtcaacgcga aattctggga cggactgccg gcggacatcc
      601 gcactgcgct cgaagacgcg atgcgcgatg cgacgcgcta cgccaacgcg atcgcgcgcc
      661 aggagaacga ccaggcgctg gccggcataa aggctgcggg caagaccgag atcatcacgc
      721 tcgacgacag gcagaaggcg gcgtggcgca aggcgatgca gcccgtgtac aaggacatgg
      781 aaagccgcgt cggcaaggac ctgatcgcag cgatccagaa ggaagcggcc gcggccgggt
      841 acaagtgagg tcgacgccgg tgaccgcgcg ctcgtacctc ttcgttccgg gtgaccggcc
      901 cgaccgcttc gcgaaggcgc tcgccagcgg cgccgacgcg gtgattgtcg acctggagga
      961 cgcggtcgcg ccggaggcga aggcggacgc gcgcgcgcag ctcgccgcat ggctgcgcgt
     1021 ggcgaccgcg cgcgtgatcg tgcgcatcaa cggcgccgac accgaatggt tcgcggacga
     1081 tctcgcactc gccgcgttgc cggcggtggc cggcgtgatg ctgccgaagg ccgagcgcgt
     1141 cgaagacctg gcgcgcgtgg cacaggccgc gcctcgacgc acgctgctgc cgctgatcga
     1201 gaccgcgcgg ggattcgatc gcgcgcgcga gatcgcgcag gccacgagcg tggcgcggct
     1261 cgtgttcggg tcgatcgact tccagcacga cctgaatatt cgcggcgacg ccgaggagtt
     1321 gctgtatttc cgttctcagc tcgtgctggt gtcgcggctg gcgggcatcg aggcgccggt
     1381 cgacggcgtg tgcaccgcga tcgacgatgc gcagcggctc gaggacgaag cgctgcgcgc
     1441 gcggcggctg gggttcggcg ctaagttatg cattcatccg aagcaggtcg gggtcgtcaa
     1501 ccgtgccttc agtccgagcg cggaggagat cgcctgggcg cggcgtgtgc tgcaggcggc
     1561 gggcgaggcg cgcggcgcgg cgatcgcggt cgacggcaag atggtcgacc ggccggtgct
     1621 gctgcgcgcg gaagcgatcg ttcgcgaagc tgcgcgcggg gatgcgccca acgatgacgg
     1681 ccgcgactga tccgcgcgcg gacacgctgc gcaggctgca ggcgttctgg gacgagcgct
     1741 acgcggccga cgagttcgca tacggcacgg ctccgaacga tttcctggtg cagaggctcg
     1801 gccgcattgc tccgcgcggc cgcgtgctgt gcctggccga cggcgagggc cgcaacggcg
     1861 tgtggctggc gcagcagggt ctgcaggtga cgtcggtcga tgtgtcggcg cgcgggctcg
     1921 acaaggcgca ggccctggcg cggcaggccg gcgtgcatat cgacgccgtg gtgtgcgacg
     1981 tcacgcgctt cgatgtcggc accgccgcgt gggatgcgat cacttcgatc ttcctgcacc
     2041 tgccggcgaa accgcgccgc gcgctgcacg aacgctgcgc ggcggcgttg aagccgggcg
     2101 gcgtcttcat ctacgaggc
//
LOCUS       DBNHBY010000623         9990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1668889, whole genome shotgun sequence.
ACCESSION   DBNHBY010000623 DBNHBY010000000
VERSION     DBNHBY010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1668889"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3680
                     /gene="rpoB"
                     /locus_tag="AC16HB_14970"
     CDS             <1..3680
                     /gene="rpoB"
                     /locus_tag="AC16HB_14970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5608138.1"
                     /translation="NGTERVIVSQLHRSPGVFFEHDRGKTHSSGKLLFSARVIPYRGS
                     WLDFEFDPKDILYFRVDRRRKMPATILLKAIGLTPEQVLAHFFTFDSFQLAASTGGYM
                     ELVPERLRGEIARFDIADKTGKVIVAKDKRINAKHIRDMEAAGMKRIEVPEDYLLGRV
                     LAANVVDPETGEILAHANEELTEDLMKKLRVANVKDIRTIYTNDLDQGPFISNTLRID
                     ETGDQLAARIAIYRMMRPGEPPTEDAVETLFRRLFFDDETYDLSRVGRMKVNSRFGRA
                     DITGPMVLTHEDIIDTMKVLVELRNGRGEIDDIDHLGNRRVRCVGELAENQFRAGLVR
                     VERAVRERLGQAETENLMPHDLINSKPISASIREFFGSSQLSQFMDQTNPLSEITHKR
                     RVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSMSLYARLNEYGF
                     LETPYRKVVNRHVTNEISYLSAIEEGKYVIGQANAPLDKTGKFVDELVSCRQAGEFVL
                     MSPERVEYMDVAPSQIVSVAASLIPFLEHDDANRALMGSNMQRQAVPCLRPEKPFVGT
                     GVERTVAVDSGTTVQALRGGVVDFVDANRIVVRVNEDENVSGEVGVDIYNLVKYTRSN
                     QNTNINQTPIVKKGDVIAKGDVIADGASTDLGELALGQNMLVAFMPWNGYNFEDSILI
                     SEKVVSDDRYTSIHIEELNVVARDTKLGPEEITRDISNLSENQLARLDESGIVYIGAE
                     VQAGDVLVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGMNGTVIDVQVF
                     TREGIERDKRAQQIIDDALKRYRQDLNDQLRIVEADAFVRIEKLMLKKEATGGPRKIA
                     KGTIITKDYLDSLEKYHWFDIRLADEAAAGQLEALKESIETKRRQFDQAFEDKRKKLT
                     QGDELPPGVLKMVKVYLAVKRRLQPGDKMAGRHGNKGVVSKITPVEDMPYMADGRPVD
                     IVLNPLGVPSRMNVGQILETHLGWAARGLGLRIGELLQSANVAQARSLLREIYNKSGK
                     PEEIDAMTDDEVVQLAEHLRDGVPFATPVFDGASESEIRAMLDMAYPADVAAKAQLTQ
                     SKTQATLYDGRTGDAFERPVTIGYMHMLKLHHLVDDKMHARSTGPYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGASYTLQEMLTVKSDDVNGRTKVYENIVKGEHSIDAGMPE
                     SFNVLVKEIRSLGIDIDLERS"
     gene            3711..7946
                     /gene="rpoC"
                     /locus_tag="AC16HB_14975"
     CDS             3711..7946
                     /gene="rpoC"
                     /locus_tag="AC16HB_14975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR5608139.1"
                     /translation="MKALLDLFKQVNKQDEQFDAIKIGIASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRER
                     MGHIELASPVAHIWFLKSLPSRMGMVLDMTLRDIERVLYFEAFCVVDPGFTPLKRGQL
                     MTEDDFIAKTEEYGDEFRALMGAEAIRELLRAIDVDKEIETLRKDLSETGSEAKIKKF
                     AKRLKTLEGFQRSGIKPEFMILEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKG
                     KGGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNKLELMGLA
                     TTIKAAKKMVDSQEPIVWDILEEVIRQHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLSLEAQLEARALMLASNNVLFPSNGDPSIVPSQDVV
                     LGLYFATREKINGKGEGGFFTDVAEVHRAYESRQVELQTRITVRIREFDVDRETGEKS
                     PRITRYETTVGRALLSEILPAGLPFSVLNKPLKKKEISRLINTAFRKCGLRETVIFAD
                     RLLQSGFRLATRAGLSIAIDDMLVPAQKEEIIRAAEKEVKEIEAQYTSGLVTQGERYN
                     KVVDIWGRAGDQVGKVMMEQLATEQTVDRHGRDTRQESFNSIFMMADSGARGSVAQIR
                     QLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVTEDDCGTTNGVAMRALVEGGEVIESLRDRILGRVAALDLINPDTQ
                     DVLLPAGTLLDEDAVDRIEQVGLDEVKVRTPLSCDTRYGICAKCYGRDLGRGSLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSNGTVRFTATMRYVTNA
                     KGEQVVISRSGEVMIVDDNGRERERHKVPYGATLLVIDGKAVKAGTQLANWDPLTRPI
                     ITEYGGTVKFENIEEGVTVAKQIDEVTGLSTLVVIDAKRRGATARGLRPQVKLINEAG
                     EEVRIASTDHAVTISFPVGALIVVRDGQQVAVGEVLARIPQESQKTRDITGGLPRVAE
                     LFEARSPKDAGMLAEVTGTVSFGKDTKGKQRLIITDLDGKEHEFLIPKDKHVLVHDGQ
                     VVNKGELIVDGPADPHDILRLIGVEALARYIVDEVQDVYRLQGVKINDKHIEVIVRQM
                     LRRVQVTDPGDAPFITGEQVERSELLDENDKAVAKGGRPALYENILLGITKASLSTDS
                     FISAASFQETTRVLTEAAIMGKTDGLRGLKENVIVGRLIPAGTGLAYHRARKDKEEAE
                     AAEREALAALEASTSPAEALEGGGEDATEVSEP"
     gene            8246..8623
                     /gene="rpsL"
                     /locus_tag="AC16HB_14980"
     CDS             8246..8623
                     /gene="rpsL"
                     /locus_tag="AC16HB_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR5608140.1"
                     /translation="MPTINQLVRKPREFARAKSKSPALKTSPQKRGVCTKVYTTTPKK
                     PNSALRKVAKVRLVNGYEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQSRSKYGAKRPKAA"
     gene            8747..9217
                     /gene="rpsG"
                     /locus_tag="AC16HB_14985"
     CDS             8747..9217
                     /gene="rpsG"
                     /locus_tag="AC16HB_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR5608141.1"
                     /translation="MPRRREVPKREILPDPKFHSADLTKFVNVIMLDGKKAVAERIVY
                     GALAQIEQKAGKNAIEVFSQALNNVKPLVEVKSRRVGGANYQVPVEVRPVRRMALAMR
                     WLREAAKKRGEKSMPQRLAGELLEAAEGRGGAMKKREEVHRMAEANKAFAHFRF"
     gene            9278..>9990
                     /locus_tag="AC16HB_14990"
     CDS             9278..>9990
                     /locus_tag="AC16HB_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5608142.1"
                     /translation="MPRKTPIERYRNIGISAHIDAGKTTTTERILFYTGVSHKMGEVH
                     DGAATMDWMEQEQERGITITSAATTTFWKGMDLSFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYRVPRLAFVNKMDRTGANFFKVYEQMRA
                     RLKANPVPIQVPIGAEENFKGVVDLVKMKAIFWDDSTQGMKFEYRDVPAELLDTCKEW
                     REKMLEAAAEANEELMNKYL"
BASE COUNT         2013 a         3076 c         3382 g         1519 t
ORIGIN      
        1 tcaatggcac cgagcgcgtg atcgtgtcgc aactgcaccg ctcgcccggc gtgttcttcg
       61 agcatgaccg cggcaagacg cattcgagcg gcaagttgct gttctcggcg cgtgtcatcc
      121 cgtaccgcgg gtcgtggctc gacttcgagt tcgacccgaa ggacatcctg tatttccgcg
      181 tcgatcgccg ccgcaagatg ccggccacga ttctgctgaa ggcgatcgga ctgacgccgg
      241 agcaagtcct ggcgcatttc ttcacgttcg acagctttca gctggcagcg tcgaccggcg
      301 gctacatgga actcgtgccg gagcggctgc gcggcgagat cgctcgcttc gacatcgccg
      361 acaagaccgg caaggtaatc gtcgccaagg acaagcgcat caatgccaag cacatccgcg
      421 acatggaggc ggcgggcatg aagcgcatcg aagtgcccga ggactacctg ctcggccgcg
      481 tgctggcggc caacgtggtc gatccggaga ccggcgaaat tctcgcgcac gcaaacgagg
      541 agttgaccga agacctgatg aagaagctgc gggtggccaa cgtcaaggac atccgcacga
      601 tctacaccaa cgacctcgat cagggcccgt tcatctcgaa cacgctgcgc atcgacgaaa
      661 cgggcgatca gttggcggcg cgcatcgcga tctaccggat gatgcgtccc ggcgagccgc
      721 cgaccgagga cgcggtcgag acgctgttcc ggcgtctgtt cttcgacgac gagacgtacg
      781 acctgtcgcg ggtcggccgc atgaaggtca acagccgctt cggccgcgcc gacatcaccg
      841 ggccgatggt gctgacgcac gaggacatca tcgacacgat gaaggtgctc gtggaactgc
      901 gcaacggccg cggcgagatc gacgatatcg accacctcgg caaccgccgc gtgcgctgcg
      961 tcggcgagct ggcggagaac cagttccgcg ccggactggt gcgtgtcgag cgcgcggtgc
     1021 gcgagcgcct cggccaggcc gagacggaga acctgatgcc gcacgacctg atcaacagca
     1081 agccgatcag cgcatccatt cgcgagttct tcggttcgtc gcagttgagc cagttcatgg
     1141 accagaccaa cccgctgtcg gagatcacac acaagcggcg cgtctccgca ctggggccgg
     1201 gtggactgac gcgcgagcgc gccggcttcg aggtgcgcga cgtgcatccg acccattacg
     1261 gccgcgtgtg cccgatcgag acgcccgagg gcccgaacat cggcctgatc aattcgatgt
     1321 cgctgtacgc gcggctgaac gagtacggct tcctcgagac gccgtaccgc aaggtcgtca
     1381 accggcacgt gaccaacgag atctcgtacc tgtcggcgat cgaggaaggc aaatacgtga
     1441 tcgggcaggc gaatgcgccg ctcgataaga ccggcaagtt cgtcgacgaa ctcgtgagct
     1501 gccgtcaggc tggcgagttc gtgctgatga gccccgagcg cgtcgagtac atggacgtgg
     1561 cgccgagcca gatcgtgtcg gtcgcggcgt cgctgatccc gttcctcgag cacgacgacg
     1621 ctaaccgcgc gctgatgggc tcgaacatgc agcgccaggc agtgccctgc ctgcgtcccg
     1681 aaaagccgtt cgtcggcacg ggcgtcgaac gcacggtcgc ggtcgactcc ggtacgacgg
     1741 tgcaggcgct gcgcggcggc gtggtcgatt tcgtcgatgc caaccgcatc gtcgtgcgcg
     1801 tgaacgagga cgagaacgtt tccggcgagg tcggcgtcga tatctacaac ctggtcaagt
     1861 acacgcgttc gaaccagaac acgaacatca accagacgcc gatcgtcaag aaaggcgacg
     1921 tgatcgccaa gggtgacgtg atcgccgacg gcgcgtcgac cgatctgggc gaactggccc
     1981 tcggccagaa catgctggtc gcgttcatgc cgtggaacgg ctacaacttc gaggactcga
     2041 tcctgatctc ggagaaggtg gtttccgacg accgctacac gtcaatccac atcgaagagc
     2101 tgaacgtcgt cgcgcgcgac accaagctcg gacccgaaga gatcacgcgc gacatctcga
     2161 acctctccga gaaccagctg gcgcggctcg acgagtcggg catcgtatat atcggtgctg
     2221 aagtgcaggc gggcgatgtg ctggtcggca aggtcacgcc gaagggcgaa acccagctga
     2281 cgccggagga gaagctgctg cgcgcgatct tcggcgagaa ggcgagcgat gtgaaggaca
     2341 cgtcgctgcg cgtgccgtcg ggcatgaacg gcaccgtgat cgacgtgcag gtcttcacgc
     2401 gcgaaggcat cgagcgcgac aagcgcgcgc agcagatcat cgacgacgcg ctgaagcgct
     2461 atcggcagga cctgaacgac cagctgcgca tcgtcgaggc ggacgccttc gtgcgcatcg
     2521 aaaagctgat gctgaagaag gaggccaccg gcggcccgcg caagatcgcc aagggcacga
     2581 tcatcaccaa ggactacctc gactcgctcg agaagtacca ctggttcgac atccggctcg
     2641 ccgacgaggc ggccgccggc cagctcgagg cgctcaagga atcgatcgag accaagcgcc
     2701 gccagttcga ccaggcattc gaggacaagc gcaagaagct cacgcagggc gacgagctgc
     2761 cgccgggtgt gctgaagatg gttaaggtct acctggccgt caagcgccgc ctgcagccgg
     2821 gcgacaagat ggcgggtcgg cacggcaaca agggcgtggt atcgaagatc acgccggtgg
     2881 aggacatgcc gtacatggcc gacgggcgcc cggtcgatat cgtgctgaat ccgctcggtg
     2941 tgccgtcgcg gatgaacgtc ggccagatcc tcgagacgca tttgggctgg gccgcgcgcg
     3001 gcctcggcct gcgcatcggc gagctgctgc aatcggccaa cgtcgcgcag gcgcgttccc
     3061 tgctgcgcga gatctacaac aagtccggca agccggagga aatcgacgcc atgaccgacg
     3121 acgaggtggt gcaactcgcc gagcacctgc gcgacggtgt gccgttcgcc accccggtgt
     3181 tcgacggcgc gtcggagtcg gagatccgcg cgatgctcga catggcctat ccggccgacg
     3241 tggccgcgaa ggcgcagctg acgcagagca agacgcaggc gacgctgtac gacggccgca
     3301 ccggcgacgc gttcgagcgt ccggtcacga tcggctacat gcacatgctg aagctgcacc
     3361 acctggtcga cgacaagatg cacgcgcgtt cgaccggccc ctactcgctg gtcacgcagc
     3421 agccgctcgg cggcaaggcg cagttcggcg gccagcggtt cggcgaaatg gaggtgtggg
     3481 cgctcgaggc gtacggcgcc agctacacac tgcaggagat gctcaccgtg aagtcggacg
     3541 acgtgaacgg ccgcacgaag gtgtacgaaa acatcgtcaa gggcgagcac tcgatcgacg
     3601 ctggcatgcc ggaatcgttc aacgtgctgg tcaaggaaat ccgttcgctc gggatcgaca
     3661 tcgatctgga acgcagctaa cggaacccga cggataccgc aggagaaagc atgaaagcgc
     3721 ttctcgatct attcaagcag gtcaacaagc aggacgagca gttcgacgcg atcaagatcg
     3781 gcatcgcgtc gccggacaag atccgctcgt ggtcgttcgg cgaagtgaag aagcccgaga
     3841 cgatcaacta ccggaccttc aagccggagc gcgacggcct cttctgcgcc aagatcttcg
     3901 ggccgatcaa ggactacgag tgcctgtgcg gcaagtacaa gcgccttaag caccgcggcg
     3961 tgatctgcga gaagtgcggc gtcgaggtga cgctagccaa ggtccggcgc gagcgcatgg
     4021 gtcacatcga gctcgcgagc cccgtggcgc acatctggtt cctcaagagc ctgccgtcgc
     4081 ggatgggcat ggtgctcgat atgacgctgc gcgatatcga gcgcgtgctg tacttcgagg
     4141 ctttctgcgt ggtcgacccg ggcttcacgc cgctcaagcg cggccagctg atgaccgagg
     4201 acgatttcat cgccaagacc gaggagtacg gcgatgagtt ccgcgcgctg atgggcgcgg
     4261 aggcgattcg tgagctgctg cgcgcgatcg acgtcgacaa ggagatcgag acgctgcgca
     4321 aggacctgtc ggagacgggg tcggaagcga agatcaagaa attcgccaag cgcctgaaga
     4381 cgctggaagg attccagcgc tccggcatca agcctgagtt catgatcctc gaagtgctgc
     4441 cggtgctgcc gccggagctg cgcccgctgg tgccgctgga cggtggtcgc ttcgcgactt
     4501 cggacctgaa cgacctgtac cggcgcgtga tcaaccgcaa caaccggctg aagcggctgc
     4561 tggagctgaa ggcgcccgag atcatcgtcc gcaacgagaa gcgcatgctg caggaggcgg
     4621 tggactcgct gctcgacaac ggccgccgcg gcaaggcgat gacgggagcc aacaagcgcc
     4681 cgctgaagtc gctcgcagac atgatcaagg gcaagggcgg gcgtttccgc cagaacctgc
     4741 tcggcaagcg cgtcgactat tcgggccgct cggtgatcgt ggtcggcccg caactgaagc
     4801 tgcatcagtg cggcctgccg aagttgatgg cattggagct cttcaagccg ttcatcttca
     4861 acaagctcga gctgatgggg ctcgcgacga cgatcaaggc cgccaagaaa atggtcgatt
     4921 cccaggagcc catcgtgtgg gacatcctgg aagaggtgat ccgccagcac ccggtgatgc
     4981 tgaaccgtgc tccaacgttg caccgtctcg gcatccaggc ctttgagccg gtgctgatcg
     5041 agggcaaggc gatccaactg cacccgctgg tgtgcgccgc gttcaacgcg gacttcgacg
     5101 gcgaccagat ggcggtgcac gtgccgctgt cgctggaagc ccagctcgag gcgcgcgcgc
     5161 tgatgctggc gtcgaacaac gtgctctttc catccaacgg cgatccgtcg atcgtgccgt
     5221 cgcaggacgt cgtgctcggc ctttacttcg ccacgcgcga gaagatcaac ggcaagggtg
     5281 aaggcgggtt cttcaccgac gtggccgagg tgcaccgcgc gtacgaaagc aggcaggtcg
     5341 aactgcagac acgcatcacc gtgcgcatcc gcgagttcga cgtcgaccgc gagaccgggg
     5401 agaagtcgcc tcgcatcacg cgatacgaaa ccacggtcgg ccgcgcgctg ttgtccgaga
     5461 tcctgcccgc ggggctgccc ttcagcgtgc tgaacaagcc gctgaagaag aaggagatct
     5521 ctagactcat caacaccgcg ttccgcaagt gcggtctgcg cgagaccgtc atcttcgccg
     5581 accggctgct gcagtccgga ttccgactgg ccacgcgcgc cgggctatcg atcgcaatcg
     5641 acgacatgct ggtgccggcg cagaaggaag agatcatccg cgccgccgag aaggaagtga
     5701 aggagatcga ggcgcagtac acctcgggcc tggtaacgca gggcgagcgc tacaacaagg
     5761 tagtcgacat ctggggtcgc gccggcgacc aggtcggcaa ggtgatgatg gagcagcttg
     5821 ccaccgagca gaccgtcgac cgccacgggc gtgatacgcg ccaggaatcg ttcaactcga
     5881 tcttcatgat ggccgactcc ggtgcgcgcg gttcggtggc gcagatccgc cagctcgcgg
     5941 gcatgcgggg actgatggcg aagcccgacg gctcgatcat cgagacgccg atcacggcga
     6001 acttccgcga aggcctgaac gtgctgcagt acttcatctc gacgcacggt gcgcgcaagg
     6061 gtctcgcgga cactgcgttg aagacggcca actccgggta cctcacacgt cgcttggtcg
     6121 acgtgacgca ggacctcgtc gtgaccgagg acgattgcgg caccaccaac ggcgtggcga
     6181 tgcgcgcgct ggtcgaaggc ggcgaggtga tcgagtcgct gcgcgaccgc atcctcggcc
     6241 gggtcgcggc gctcgacctg atcaatccgg atacgcagga cgtgctgctt ccggccggaa
     6301 cgctgctgga cgaggacgcg gtggaccgaa tcgagcaggt cggactcgac gaagtcaagg
     6361 tgcgcacgcc tctcagctgt gacacgcgtt acggcatatg cgccaagtgt tacggccgcg
     6421 atcttggccg cggctcgctc gtcaatgcag gcgaggcggt cggcgtgatc gctgcgcagt
     6481 cgatcggtga gccgggcacg cagctgacca tgcgcacgtt ccatatcggc ggcgccgcct
     6541 cgcgcgcggc cgtggcgagc agcgtcgagg ccaagagcaa tggcacggtg cgcttcaccg
     6601 cgacgatgcg ctacgtcacc aatgccaagg gcgagcaggt ggtgatctcg cgctcgggcg
     6661 aagtgatgat cgtcgacgac aacggtcgcg agcgcgagcg ccataaggtg ccgtacggcg
     6721 cgacgctgct cgtcatcgac ggcaaggcgg tcaaggccgg aacgcagctg gcgaactggg
     6781 atccgctgac gcgcccgatc atcacggagt acggcggcac cgtgaagttc gagaacatcg
     6841 aggagggagt caccgtcgcc aagcagatcg atgaggtcac cggcctgtcc acgctcgtcg
     6901 tgatcgacgc caagcgccgc ggcgcgactg cgcgcggcct gcggccgcag gtcaagctga
     6961 tcaacgaagc cggcgaggaa gtcaggatcg ccagcaccga tcacgcagtg acgatcagct
     7021 tccccgtcgg cgcgctgatc gtcgtgcgcg atggccagca ggttgcggtc ggcgaggtgc
     7081 tggcgcgcat tccacaggag tcgcagaaga cgcgcgacat caccggcggt ttgccgcggg
     7141 tggcggaact gttcgaggcg cgctcgccca aggacgcggg catgttggcg gaggtgaccg
     7201 gcaccgtctc gttcggcaag gacaccaagg gcaagcagcg cctgatcatc accgacttgg
     7261 acggcaagga gcacgagttc ctgatcccga aggacaagca cgtgctggtg cacgacggcc
     7321 aggtggtgaa caagggcgaa ctcatcgtcg acggtccggc cgatccgcac gacatcctgc
     7381 gcctgattgg cgtcgaggcg ctggcgcgct acatcgtcga cgaagtgcag gacgtctacc
     7441 gtctgcaggg cgtgaagatc aacgacaagc acatcgaagt gatcgtgcgg cagatgctgc
     7501 gtcgggtgca ggtcaccgat ccgggcgacg cgccgttcat cacgggcgag caagtcgagc
     7561 gttccgaatt gctggacgag aacgacaagg cggtcgccaa gggcggacgt ccggcgctgt
     7621 acgagaacat ccttctcggc atcaccaagg cgtcgttgtc gaccgactcc ttcatctcgg
     7681 cggcgtcgtt ccaggaaacg acgcgcgtgc tgaccgaagc cgcgatcatg ggcaagacgg
     7741 atggcctgcg cgggctgaag gaaaacgtaa tcgtcggcag gctgattccg gcgggcaccg
     7801 ggctcgcgta tcaccgcgcg cgcaaggata aggaggaggc cgaggccgcc gagcgcgagg
     7861 cgctggcagc cctcgaggcg tccacttcac cggcagaggc actcgaaggc ggcggcgaag
     7921 atgccaccga ggtgtcggaa ccctgaccga gcgcgggacc tgagtcaaga cgcggccggc
     7981 cgagcgccgg ccgcgttcaa ttcggggcct ttcctcgcct tgacaccgat ggcgaagaaa
     8041 gacgaaaatg caaggctttc tggagcccgc ccgggcccgg tgttcttttc gcttcggcgc
     8101 cacggcaagt tggtggcgcc tgaccgttcg gggcaccctt tccggtgccg gatctccgtg
     8161 caggaatgtg atggcgcttc ggccgcggag gcggagcgcc gatgaatcac ggaacccaag
     8221 tcactgaacc gcaggcgaaa gaacaatgcc gacgatcaat caactagtgc gcaagccgcg
     8281 cgaattcgcg cgcgcgaaga gcaaaagccc ggcgctcaaa acgagcccgc agaagcgcgg
     8341 cgtgtgtacc aaggtctaca cgacgactcc caagaagccg aactcggcgc tgcgcaaggt
     8401 cgccaaggtg cgcctggtca acggctacga ggtgatctcg tacatcggcg gcgagggcca
     8461 taacctgcag gagcactcgg tggtgctgat caggggcggc cgcgtcaagg acctgccggg
     8521 cgtgcgctac cacatcgttc gcggttcgct cgacacgcag ggcgtgaagg accgcaagca
     8581 gagccgatcg aaatacggtg ccaagcgccc gaaggcagcc tgaggaccga ttggaccaag
     8641 aggccgctgc gctgcaaggc gagcggtcga gtaagtcgcg gcccggatga aggggtcgcc
     8701 aacgccgccc tgggcggctt acgactgaag ccagagagga agtgtcatgc cccgtcgccg
     8761 cgaagttccc aagcgggaaa tcctgccgga tccgaagttc cacagcgcgg atctgaccaa
     8821 gttcgtcaac gtcatcatgc tcgacggaaa gaaggccgtc gctgagcgca tcgtctacgg
     8881 cgccctggcg cagatcgagc agaaggcggg taagaacgcg atcgaggtgt tctcgcaggc
     8941 gctgaacaac gtcaaaccgc tggtcgaggt gaagagccgt cgcgtcggcg gcgccaacta
     9001 ccaggtgccg gtcgaggtgc gcccggtgcg acggatggcg ctggcgatgc gctggctccg
     9061 cgaggcggca aagaagcgcg gcgaaaagtc gatgccgcag cgcctcgccg gtgagttgct
     9121 cgaagccgcg gaaggccgcg gtggcgcgat gaagaagcgc gaggaagttc accgcatggc
     9181 cgaggccaac aaggctttcg cgcactttag gttctaacgg tcgggtaagc gaggcacgct
     9241 gcgcgcgcct cgtactcaag tttttcggga caaccgtatg ccacgcaaga cgcctatcga
     9301 acggtatcgg aacatcggta tctccgcgca catcgacgcc ggcaagacga cgacgacgga
     9361 gcgcatcctt ttctataccg gcgtcagtca caagatgggc gaggtgcacg acggcgccgc
     9421 gacgatggac tggatggagc aggagcagga gcgcggcatc acgatcacgt cggccgccac
     9481 tacgacgttc tggaaaggga tggatctgtc gtttcccgag caccggatca acatcatcga
     9541 cacgccgggc cacgttgatt tcacgatcga ggtcgagcgc agcatgcgtg tgctcgacgg
     9601 cgcgtgcatg gtgtactgcg cggtcggcgg cgtgcagccg cagtcggaga ccgtctggcg
     9661 ccaggccaac aagtaccgcg tgccgcggct ggcgttcgtg aacaagatgg accggaccgg
     9721 cgccaacttc ttcaaggtct acgagcagat gcgcgcgcgc ctgaaggcca atccggtgcc
     9781 gatccaggtg ccgatcggcg ccgaggagaa cttcaagggc gtggtcgacc tggtcaagat
     9841 gaaggcgatc ttctgggacg actcgaccca gggcatgaag ttcgaatacc gcgacgtccc
     9901 ggccgaactg ctggacacgt gcaaggaatg gcgcgagaag atgctggagg cggcggccga
     9961 ggcgaacgag gaactgatga acaagtacct
//
LOCUS       DBNHBY010000624         4679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_111111, whole genome shotgun sequence.
ACCESSION   DBNHBY010000624 DBNHBY010000000
VERSION     DBNHBY010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_111111"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="AC16HB_14995"
     CDS             complement(<1..1044)
                     /locus_tag="AC16HB_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608143.1"
                     /translation="MKALLPGTPRLQLRGIRKVYPTVVANDGIDLTVMPGEIHAVLGE
                     NGVGKSTLMKVIYGVTRPTAGEIWWEGRPVHIASPAQARALGIGMVFQHFSLFETLTV
                     VENVALGMPETEDLDDLAARIGRVSEKYGLPVDPRRLVHALSVGERQRVEIVRCLLQN
                     PRLLIMDEPTSVLTPQAVRKLFQTLRRLAAEGCSILYISHKLDEIQELCDVATVLRAG
                     KVAGTCIPSQETPQSMARLMIGRDLPVCRHEAPAQGGEVVLRLSGLSHASEDRFGTDL
                     KDIHLEVRAGEIVGIAGVSGNGQQELLRAISGEEPQAEKHPVQICGTEVGRMTPGRRR
                     QLGLGFVPEERLGR"
     gene            1243..1827
                     /locus_tag="AC16HB_15000"
     CDS             1243..1827
                     /locus_tag="AC16HB_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR5608144.1"
                     /translation="MGKTRLEAFSDGVIAIIITIMVLEMKVPHGESLDTLLPLWPVFL
                     SYVLSFLYVGIYWNNHHHMLHATGKVSGGVLWANLHLLFWLSLFPFVTGWMGENHFAQ
                     APSALYGVVLLMAALAYLVLQQTIIATHGPESVLRKAIGRDWKGKLSPLMYLLAIAST
                     FWSAWLAQAIYVAVALAWLVPDRRIERVLRGERE"
     gene            1996..3144
                     /locus_tag="AC16HB_15005"
     CDS             1996..3144
                     /locus_tag="AC16HB_15005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase family protein"
                     /protein_id="HIR5608145.1"
                     /translation="MSDAAIRPFRLSVPQGAIDDLHARLDRVRWPDEAPDAPWAYGTS
                     LAFMRELVHDWRTRYDWRTTEAELNAWPQFVTEIDGIDIHFLHVQGKGPHPKPLLLSH
                     GWPGSVLEFMKLIPRLTDPAAHGGAAADAFTVVVPSLPGYTLSFRPGQPRKGLAEIGA
                     IFDTLMHARLGYRRYGAQGGDWGSFITAWLGRNRAEHLAGIHLNLMPLKRDAAMFTNP
                     SENERRYVDELQAWLKEETGYQAIQGTRPQTLAFALSDSPIGLAAWISEKFRAWTDCK
                     GDPRHALTMDEMLGDISLYWFTNCIGASFWPYYARLHGPWPIEGRIEVPTGYCEFPRE
                     ILRPPREAAERQFNIVRWSVMPNGGHFAALEQPDALAHEIREFFRGIA"
     gene            complement(3158..3937)
                     /locus_tag="AC16HB_15010"
     CDS             complement(3158..3937)
                     /locus_tag="AC16HB_15010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608146.1"
                     /translation="MAERVALVTGAARGIGLAIAHWFHARDWRVALLDKDGPALTRAA
                     GEFAGSSRVLPLYSDVSQPDQVQAAVARTAERFARIDALINNAGVAVFKPILQTTFED
                     WLFVLATNLNGPFLCTQACAPVMQSNGGGSVVNIASISGLRASTLRVAYGTSKAALIH
                     LTRQQATELGTMGIRVNAIAPGPVETEMAKQVHTAAIRKDYHDVIPLGRYGTVEEVAH
                     AVGFLCSPEASYINGQVIAVDGGFDAAGVGLPTLRRNAAGG"
     gene            complement(3948..4652)
                     /locus_tag="AC16HB_15015"
     CDS             complement(3948..4652)
                     /locus_tag="AC16HB_15015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608147.1"
                     /translation="MNASTTLITGAGRGIGLATAKSFLAAGWHVLALDKDFSRFDLAA
                     SPQVRRVQYDLRALDGIPALISSLGRIDTLVNNAAVLYCDPFDAIPAAHKQEIFTVNL
                     EAPVALIEAVAPQMMARRGGRIVNVGSVAAFTGHPDLWYGATKAALLNVTKSYAATLG
                     KRGVLVNAVAPGPTQTDMYEQLPQSRKDGVMRSVHAGRVCQPEEVAAAIFWLGSASPE
                     YVSGTTLDVNSGSYPR"
BASE COUNT          735 a         1621 c         1535 g          788 t
ORIGIN      
        1 gcgcccgagg cgctcctccg gcacgaagcc caagccgagc tggcgccgcc gccccggcgt
       61 catgcggccg acctcggtgc cgcagatctg caccggatgc ttctcggcct gcggttcctc
      121 gccagagatc gcgcgcagca gttcctgctg cccgttgccg gacacgccgg cgatgccgac
      181 gatctcgccc gcgcgcactt cgagatgaat gtccttcagg tcggtcccga accgatcttc
      241 gctcgcatgc gacaggcccg acagacgcag cacgacttcg ccgccctgcg ccggcgcctc
      301 gtgccgacac accggcaggt ccctgccgat catcagccgc gccatcgact gcggcgtctc
      361 ctgcgacggg atgcaggtgc cggcgacctt gccggcgcgc agcacggtcg cgacatcgca
      421 cagctcctgg atctcgtcca gcttgtggct gatgtacagg atcgagcaac cttcggcagc
      481 gagacgccgc agcgtctgga agagcttgcg gaccgcctgc ggcgtcagaa ccgaggtcgg
      541 ctcgtccatg atcagcaggc gcgggttctg cagcagacag cgcacgattt ccacgcgctg
      601 ccgttcgccg accgacagcg cgtgcaccag ccggcgcgga tcgaccggca acccgtactt
      661 ctcggacact cgcccgatcc gcgcggcgag atcgtcgagg tcctcggtct cgggcatacc
      721 gagcgcgacg ttctcgacca cggtcagcgt ctcgaacagg gaaaaatgct ggaacaccat
      781 gccgatgccc agcgcgcgtg cctgagcggg gcttgcgatg tggaccggcc tgccttccca
      841 ccagatctcg cccgcggtgg gccgggtcac gccgtagatg accttcatca gcgtcgactt
      901 gccgacaccg ttttcgccga gcaccgcgtg gatctcgccc ggcatcaccg tgaggtcgat
      961 gccgtcgttg gcgaccacgg tcgggtacac cttgcggatg ccgcgcagtt gcagccgcgg
     1021 cgttccaggt agcagtgcct tcacgctgtg tctgtctgtc cgtcgcgcac gccgggcgct
     1081 gcgccgattc ccgttactcg agtgccgccg tcgcgcgcac cggcgaacta ccggaatcgt
     1141 agcgcctgga cccggttcac gtgccggccg tgcggtccgg cgggctgcag tcgcgcccgg
     1201 ccgaactcct cagccaccgg ggaaaccgtt aacctgcgca cgatgggaaa gacgcggctc
     1261 gaagccttca gcgatggcgt gatcgcgatc atcatcacga tcatggtgct ggagatgaag
     1321 gtgccgcacg gcgaaagtct cgacaccctg ctgccgctct ggccggtgtt cctcagctac
     1381 gtgctcagct tcctctacgt cggcatctac tggaacaacc accatcacat gctgcacgcg
     1441 accggcaagg tcagcggcgg cgtgctgtgg gccaatctgc acctgctgtt ctggctgtcg
     1501 ctgttcccgt tcgtcaccgg ctggatgggc gagaaccact tcgcgcaggc gccgtcggcg
     1561 ctgtacggcg tcgtgctgct gatggccgcg ctcgcgtacc tggtcctgca gcagacgatc
     1621 atcgcgaccc atggccccga gtccgtcctg cgcaaggcga tcggtcgcga ctggaagggc
     1681 aagctttcgc cgctcatgta cctactcgcc atcgcgtcga cgttctggtc ggcctggttg
     1741 gcgcaagcca tctacgtcgc ggtggcgctg gcgtggctcg tgccggatcg ccgcatcgag
     1801 cgggtgctgc ggggcgagcg ggaatagaca gcgcccgagg caacgctcga cgcgttgccg
     1861 cacgatcttc gcgtcgtgct tggatttcga tttccggcag gttcgcggag tttcgtcgac
     1921 ggtcctcgaa tgcgacgcgg caagttgcca gcccggggtg caacattcgt tcgtcatcga
     1981 ccgagggtgc ccgccatgtc cgatgccgct atccgaccgt ttcgtctctc ggtgccgcaa
     2041 ggcgcgatcg acgacctgca cgctcgcctc gaccgggtcc gctggcccga cgaggcgccc
     2101 gacgcgccgt gggcctacgg cacgtcgctc gcgttcatgc gcgaactggt ccacgactgg
     2161 cgcacgcgct acgactggcg cacgaccgag gccgaactca acgcgtggcc gcaattcgtc
     2221 accgagatcg acggaatcga catccacttc ctgcacgtgc agggcaaggg accacatccc
     2281 aaaccgctgc tgctctcgca cgggtggccg ggctcggtgc tggagttcat gaaactgatc
     2341 ccgcggctga ccgacccggc cgcgcacggc ggtgccgcgg ccgatgcctt caccgtcgtc
     2401 gtgccctcgc tgccgggtta cacgctttcg ttcaggccgg ggcagccgcg caagggcctc
     2461 gcggagatcg gcgcgatctt cgacacgctg atgcacgcgc ggctcggcta tcgccgttac
     2521 ggcgcgcagg gcggcgactg ggggtcgttc atcaccgctt ggctgggccg caatcgcgcg
     2581 gaacaccttg cggggatcca tctgaacctg atgccgctga agcgcgatgc ggcgatgttc
     2641 acgaatccga gcgagaacga gcggcgctac gtagatgaac tgcaggcctg gctgaaggag
     2701 gaaaccggct atcaggcgat ccagggcacg cgcccacaga cgctggcgtt cgcgctgtcg
     2761 gattcaccga ttgggctggc cgcgtggatc agcgagaagt tccgcgcctg gaccgactgc
     2821 aaaggcgatc cgcgccacgc gctgacgatg gacgagatgc tcggcgacat cagcctgtac
     2881 tggttcacca actgcatcgg cgcgtcgttc tggccgtact atgcgcgcct gcacggcccg
     2941 tggccgatcg aggggcgcat cgaggtgccg accggctact gcgagttccc gcgcgagatc
     3001 ctgcgaccgc cgcgcgaagc ggccgagcgg cagttcaaca tcgtgcgctg gagtgtgatg
     3061 ccgaacggcg ggcacttcgc ggcgctggaa cagccggatg cgctcgccca tgagatccgc
     3121 gagttcttcc gcggcattgc gtagacggac actcttatca gccgcccgct gcgttccgcc
     3181 gcagcgtcgg caatcccacc ccggccgcat cgaacccgcc gtcgaccgcg atcacttggc
     3241 cgttgatgta gctcgcctca gggctgcaca ggaatccgac ggcgtgggcg acttcctcga
     3301 ccgtgccgta gcggccgagc ggaatcacgt cgtgataatc cttgcgtatc gccgcggtgt
     3361 gaacctgttt ggccatttcc gtttccaccg ggcccggtgc aatcgcattg acgcggatgc
     3421 ccatcgtgcc cagttcggtg gcctgctgcc gggtcaggtg gatcagcgcc gccttgctgg
     3481 tgccataggc cacgcgcaac gtgctggcgc gcaggcccga gatcgacgcg atgttcacca
     3541 cgctgccgcc gccgttgctc tgcatcaccg gcgcgcacgc ctgcgtgcac aggaacgggc
     3601 cgttcagatt cgtcgccagc acgaacagcc agtcctcgaa cgtcgtctgc agaatcggct
     3661 tgaacaccgc tacgcccgcg ttgttgatca gcgcgtcgat gcgtgcgaat cgctccgccg
     3721 tccgcgcgac ggcggcctgc acctgatcgg gctgcgagac gtcgctatac agcggaagaa
     3781 ctcgcgacga cccggcgaat tcccctgcgg cgcgcgtaag cgctggccca tccttgtcca
     3841 gcagcgccac gcgccagtcg cgcgcatgga accagtgcgc gatcgccagc ccgatgccgc
     3901 gcgcggcgcc ggtgacgaga gcgacccgct cggccacgga ttcctcctta gcgcgggtac
     3961 gagccgctgt tgacgtcgag cgtggtgccg ctcacgtact cggggctggc actgccaagc
     4021 cagaagatcg cggcggcgac ttcctcgggc tggcagacgc ggcccgcgtg cacgctgcgc
     4081 atcacgccgt ccttgcgcga ctgcggcaac tgctcgtaca tgtcggtctg cgtcggaccg
     4141 ggcgcgaccg cattgacgag cacgccccgc ttgccgagcg tcgcggcgta gctcttggtc
     4201 acgttcagca gcgcagcctt ggtcgcgccg taccacaggt cgggatgacc ggtgaaagct
     4261 gcgaccgacc cgacgttgac gatgcggccg cccctgcgcg ccatcatctg cggcgcgacg
     4321 gcttcgatca gcgccaccgg cgcttcgaga ttgaccgtga agatctcctg cttgtgcgcg
     4381 gccgggatcg catcgaacgg atcgcaatac agcacggcgg cgttgttcac cagcgtgtcg
     4441 atccggccaa gcgaggagat cagcgcgggg attccgtcca gggcgcgcag gtcgtattgc
     4501 acgcggcgga cctgcggcga tgcggcgaga tcgaagcggg agaagtcctt gtcgagcgcc
     4561 agcacgtgcc agccagcagc gagaaacgat ttcgccgtcg caagcccgat gccgcggccg
     4621 gcacccgtga tcaaggtggt ggacgcattc accgcaggga ttctatcgag cgcgtccca
//
LOCUS       DBNHBY010000625         3878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_923863, whole genome shotgun sequence.
ACCESSION   DBNHBY010000625 DBNHBY010000000
VERSION     DBNHBY010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_923863"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..544
                     /locus_tag="AC16HB_15020"
     CDS             299..544
                     /locus_tag="AC16HB_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608148.1"
                     /translation="MVIPLLALSGAFTPREGMLEFVPHIMLGLPDTHFVMLTRTAPFC
                     GAVIEAAWPQVAALALIGSMLFVVSPVRWRRTPGSTA"
     gene            541..1560
                     /locus_tag="AC16HB_15025"
     CDS             541..1560
                     /locus_tag="AC16HB_15025"
                     /inference="COORDINATES: protein motif:HMM:NF021497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HIR5608149.1"
                     /translation="MKQSVWLISLALLLSGAVDCAAQSAAPYRGALTVTVENDKGTGS
                     DNNYTNGIGVSWVSSAIDTYEERSLVRRWGEFWSFLPFVGNDGYRTYVAWSIAQEMHT
                     PDDITNPNPPKDDQPYAGVLYLDNVVYARRDRWTHAWQLRVGVVGPASQAANAQEWFH
                     RVIGSDKPMGWHTQLPNEPVLNVGYTGTYRLAQGDLGKSASWKVIPVAYAGLGNYFTG
                     VGLGLYGEVGWNLVDAMGGTALRQGLNTASTVGVGPVDGWSVSLSGGFSGYGVAHYLP
                     LDGTVFRESRSVGSKPFIGMGTYGIAVRHRGFVFFLGRTYFTNTFETERHRPEFGTLS
                     LSWYF"
     gene            1981..3387
                     /locus_tag="AC16HB_15030"
     CDS             1981..3387
                     /locus_tag="AC16HB_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5608150.1"
                     /translation="MLLALALAGCATPVVAPSVDVPPRFAAATAAESQPEAAWWETFH
                     DPVLTNLVRRAARDNRDIKIAAERLRAARAGQTVSRSFLLPSVSAVGSAADRSSGYSD
                     AVKQALPDTKSAAAGLDVAWEIDLSGRLRAGSAAAAADALAAEDSVRGVRLLVMADIA
                     SNYFTLVGALRQIETLRAISAAHDETLRLVTARQRVGLATAFDVERAQTEAASARAQI
                     PPLETIAAVSRHRIAVLTGDQAFNAARIEPWRGDVVLPDIKPGQPATLLQRRPDVLAL
                     MAQLDAANARRQQAAAEWFPRLFLDASFGRQNVELNSVGLGSARFTNAAGLLAMPIFN
                     AGRTQAINEIAESGQREALLRAEDGIVRALEDVENSLVALSLERQRAQLLQTAAASSE
                     AALGRAQSLYDRGQIDLLPLLDAQRARLSVRVSANDSNTRLLLDSVQLYKALGGGWQV
                     FEPAKPPATASAPRSSQS"
     gene            3415..>3878
                     /locus_tag="AC16HB_15035"
     CDS             3415..>3878
                     /locus_tag="AC16HB_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608151.1"
                     /translation="MKTSHPAALLALPMTAILAACSPSKPPAEPLRPVRTVELRYDNA
                     AEANRYVGTVQSRHEVDQAFRVGGKVVDRRVDVGQTVREGDVIGVLDDTDYRLAEEAR
                     RQQLIAATAQARQAESDRDRLSALKTDGSVSESDEERAHSSAQSAKAAAEAKA"
BASE COUNT          641 a         1340 c         1240 g          657 t
ORIGIN      
        1 cttctgacgc tcaccaaact gcgactgaaa gtcgacgctt cactcaccac gctctgcgag
       61 cgcctggtta cgctgccctc acgtcgaatc catttgctgt cgccgatcga tgccgctact
      121 tgcaccccgt gccaagaggc ctccgcggcc cgacctcgag ccgagtcgcc catcggaatt
      181 gccgaatggg catcaagggc gcgctcagga tgtcacttgc gatcggtttg gccggttgca
      241 ccacctgaca cctgacttcg cgcgctcgat gcctcagctt ggcccgctga cgacgctcgt
      301 ggtcattccg ctgctggctc tttccggcgc cttcacgccg cgcgaaggca tgctggagtt
      361 cgtccctcac atcatgctcg gcttgccgga cacgcacttc gtcatgctca cgcggacggc
      421 gcccttctgc ggcgcagtca tcgaggcggc gtggccgcag gtcgcggcgc tcgcgctcat
      481 cggaagcatg cttttcgtcg tttcacctgt gcgctggcgc agaacgccgg ggtcgacggc
      541 atgaagcaga gtgtgtggct gatttcgctc gcgttgctgt tatccggcgc agtcgactgc
      601 gccgcgcagt cagccgcgcc ttatcgtggc gcattgacgg ttaccgtgga gaacgacaag
      661 ggaaccgggt ccgacaacaa ctacacgaac ggcattggcg tttcgtgggt gtcgagcgcc
      721 atcgacacgt acgaggaacg gagcctcgtt cgtcggtggg gcgaattctg gtcgttcctg
      781 ccgttcgttg ggaatgacgg ttaccgcacg tatgtggcct ggtccattgc acaggagatg
      841 catacgccag acgacatcac gaatccgaac ccgccaaagg acgaccaacc ctatgccggc
      901 gtgctgtatc tagacaatgt ggtctatgcg cgacgcgacc gctggacaca tgcgtggcag
      961 ctcagggtcg gtgtcgtggg ccccgcatcg caggctgcca acgcgcagga gtggttccac
     1021 cgagtgatcg gctcggataa gccgatgggc tggcacacgc agcttccgaa cgagccggtc
     1081 ctcaacgtcg gttacacggg cacgtaccgg ctggcccaag gggacttggg caaatcggcc
     1141 tcatggaaag tgattcccgt ggcctacgca ggtctcggca actacttcac cggtgtcgga
     1201 ctcggtctgt acggcgaggt cggatggaac ctcgtggatg ccatgggcgg cacagccctg
     1261 cgtcaagggc tcaatacggc ctccacggtc ggtgtcgggc cagttgacgg atggtccgtg
     1321 tcgctttccg gcgggttttc gggctacggc gtcgctcact accttccact cgacggtact
     1381 gtcttcaggg agagccgatc agtcggctcc aagcccttca tcggcatggg cacatacggt
     1441 atcgccgtgc gacatcgcgg tttcgttttc ttcctcggta gaacgtactt caccaacacg
     1501 ttcgaaaccg agcgccaccg ccccgagttc ggaactttga gcctgtcctg gtacttctga
     1561 agaagtggtg tcgccgcggc gtggatcacc cagcgccagg atgtaggtgg ctcttccccc
     1621 agattcacgc cgcgggcagc ctgcgaggag gcgccgccgc cgctgacaac gtcggcggcg
     1681 gcaccaccct ttctgccctt cggtgtgcag acccggcgca ctgacccgct cgcggcatgg
     1741 atcgatggtg gcctgcgcgc cgcgcgcttc ctttgctgac gctcaccaaa cagcgaccga
     1801 acgtcgacgc ttcagtgacg acttcgatca agccgttgga tacgctgctc acacatcgcc
     1861 actcaatgtt gtcagtgacc catgccgcta cctgttctcc aagatcagag cccttcgaag
     1921 accagcctcg aggcgaatcg cccagtgaac gggccggcac cgtcgaagac ggcgctcgca
     1981 gtgttgctgg cgctcgcgct ggccgggtgt gccacgcccg tcgtcgcgcc ttcggtcgac
     2041 gtcccccccc ggtttgcggc cgctacggct gctgagagcc aacccgaggc ggcctggtgg
     2101 gagactttcc acgacccggt gctgaccaac ctcgttcgtc gcgcggcgcg agacaatcgc
     2161 gacatcaaga tcgccgccga gcgcctgcgt gccgcgcgtg ccggccagac ggtcagccgg
     2221 tctttcctct tgcccagcgt cagcgcggtc ggctcggctg ctgaccgcag ctccggctat
     2281 agcgatgccg tcaagcaggc cctgcccgac accaagagcg ccgcggcggg cctggacgtc
     2341 gcgtgggaga tcgacctcag cggccgcctg cgcgctggct cggccgcggc cgccgccgat
     2401 gcgctggctg cggaggacag cgtgcgcggc gtgcgcctgc tggtgatggc cgacatcgcg
     2461 agcaactact tcacgctggt cggcgcgctg cgccagattg agaccctgcg cgcgatttcg
     2521 gccgcgcatg acgaaaccct gcgcctggtc accgcgcgcc agcgggtggg cctggcgaca
     2581 gcgttcgacg tcgagcgcgc gcagaccgaa gccgcatcgg cacgcgcgca gatcccgccg
     2641 ctggagacaa tcgcggcagt ctcgcggcac cgcatcgcgg ttctgaccgg cgaccaggct
     2701 ttcaacgccg cacgcatcga gccttggcgc ggcgacgtgg tcttgccgga catcaagccc
     2761 ggacagcccg ctaccctgct acagcgccgg ccggacgtgc tcgcgttgat ggcgcagctc
     2821 gatgcggcga atgcgcgccg ccagcaggcc gcggccgaat ggttcccccg gctctttctc
     2881 gatgcgtcgt tcggccgcca gaacgtggag ctgaatagcg tcggtctcgg ctccgcccgc
     2941 ttcaccaacg cggcgggcct gctcgcgatg ccgatcttca acgcggggcg cacgcaggca
     3001 atcaacgaaa tcgccgaaag tggccagcgc gaagccctgc tgcgcgccga ggatggcatc
     3061 gtgcgagcgc tggaggacgt cgagaattcg ctcgtcgcac tctcgctgga gcggcagcgc
     3121 gcgcaattgc tgcagaccgc ggcggcatcc tccgaggcag cgctcggccg tgcgcaatcg
     3181 ctctacgacc gcgggcaaat cgacctcctg ccgctgctgg acgcgcagcg cgcgcgtctg
     3241 tcggtgcgcg tgagcgcgaa cgacagcaac acccgtctgc tgctcgacag cgtgcagctc
     3301 tacaaggcgc ttggcggagg ctggcaggtg ttcgagccag ccaagccgcc ggccaccgcc
     3361 agtgcaccac gctcatccca atcctgaaac ccactgacac caccaggaca ccacgtgaag
     3421 acctctcacc ctgcggccct tctcgccttg ccgatgaccg cgatccttgc agcgtgctcg
     3481 cccagcaaac cgcccgccga gccactccgg cccgttcgca ccgtcgaact ccgttacgac
     3541 aacgccgccg aggcgaaccg ctatgtcggc accgtgcagt cgcggcacga agtcgaccag
     3601 gcgttccgcg tcggcggcaa ggttgtcgac cggcgtgtcg acgtcgggca aaccgtgcgc
     3661 gagggcgatg tcatcggcgt actcgacgac accgactacc gactcgccga agaagcgcgg
     3721 cgccagcagt tgatcgccgc cacggcgcag gcgcggcagg ccgaatcgga ccgcgaccgc
     3781 ctcagcgcgc tgaagaccga tgggtcggtg agcgagtccg acgaggaacg cgcgcacagc
     3841 agcgcgcaat ccgccaaggc ggcggccgag gccaaggc
//
LOCUS       DBNHBY010000626         6029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1669218, whole genome shotgun sequence.
ACCESSION   DBNHBY010000626 DBNHBY010000000
VERSION     DBNHBY010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1669218"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16HB_15040"
     CDS             <1..493
                     /locus_tag="AC16HB_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5608152.1"
                     /translation="CVPVCPTGASHKRPEDGIVLVDYDKCIGCKYCAWACPYGVRELD
                     AKQQVMKKCTLCVDRIYDAKLPAAERKPACVMACPTSARLFGDVHDPDSEVSIAIRER
                     AGYALMPEWGTRPANHYLPRRRTELPIAAEELQRVDNPLKIDGKLPKPAKSAPSLDDV
                     MSW"
     gene            504..1457
                     /locus_tag="AC16HB_15045"
     CDS             504..1457
                     /locus_tag="AC16HB_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HIR5608153.1"
                     /translation="MQPAFSVVFLTTLIGAGQGLLLALFVAEFGARYRWWLAAPADSG
                     FFALGAAIAVALTGLGLVASVFHLGRPERGWRAAARWRTSWLSREVIVLPAFMAVTVL
                     YGAAHWLGAEPDLRTLALGALAALLALALYLCTAMIYACLKFLQEWATPLTVANYLLL
                     GASSGFSLAAAVAALRAPQLLDFLVAWAVALTAAALASRAASLIRNARLKPRSTLQSA
                     IGVKHPRIVQKSMGFMGGSFNTREFFHGRTLSLLRAVKWTFLVLVFPVPALLLVTGLT
                     LSSSTLLIAAFLVQYAGLLAERWFFFAQANHPQNLYYQQVS"
     gene            1540..2739
                     /locus_tag="AC16HB_15050"
     CDS             1540..2739
                     /locus_tag="AC16HB_15050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5608154.1"
                     /translation="MNARDPQSESGTVAVPNAVPLTADAGRVPPHSVLFDFLGLVIRL
                     TGARAGAIRAPTPDGVSLRLMAAVGLSDEVCARDNLMPVSCGMCGAALQSLLTRQATP
                     AIGCAIHSSHATLSEFSGRAVAVPLDHLDKPIGVLNLFFAEGSDPPPGIAHLLRPFGQ
                     LLGLALENERLTRENLRVRLAAERQAMAADIHDALAQSLTFVRMRMSLLNDAIRGQDC
                     NSAQTYWNDASDELRNAHGRLREIISLYRAGMASRGLAASLIELASVFRQRTGIDLQF
                     DPPGSDFELTTEQQLQVFYILQEALANVARHSGAHEASLRTERTDDELRFIVEDDGRG
                     PSEEILDPRCAGRLTEAGHHGLQIMRERAGHAGGRLKIEALPVGGTRVTLAIHRPQRS
                     TGARALA"
     gene            2736..3407
                     /locus_tag="AC16HB_15055"
     CDS             2736..3407
                     /locus_tag="AC16HB_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608155.1"
                     /translation="MNPPPIRLVLVDDHALVRQGLADLLSRGAGMQVLATTGRAEETR
                     ALLEKYQPDLLILDLRMGPTDGITVLRDLREQGVQTPVIVLTMSEAAEDLAAALRLGV
                     RGYLLKDMEPADVIEGIRRVARGELVVAPSIAGKLSTILQSGDEPGSAASLLKQLTGR
                     ERQILGHVAAGKSNKAIAKALGISHDTVKLHVRHILAKLGLSSRVEAAVFAVQNRAVL
                     GGDVR"
     gene            complement(3685..4800)
                     /locus_tag="AC16HB_15060"
     CDS             complement(3685..4800)
                     /locus_tag="AC16HB_15060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5608156.1"
                     /translation="MNSKRISLLDPGLISAAGHHLEWDLRVVDELTSLGHQVEVFAHA
                     RADRQVVESFSGRAKLLPLFTVNPYIDPRLIDPVAGELAVFLGGSSVLAQELARIGEC
                     DLWLWPSLFAPQLFACSKVAREVRISGCIHVEPSYGADNGRMWWRYAFLMAQRARLRL
                     NVGVTGPLLQKEYAPLTAEGRIDCFPVAHDGAPIGTCKTGLTRIGFFGSQRPEKGAAM
                     LAELASRLLREGFEVVLHDSRGLVRAESSPRLTVLGYVPSLVEEIARCDVVVVPYEPA
                     AYRWKGSGIVWDAIASGIPVIVPSGTAPGLLVEETGAGKTVDVLSIDAIFRAIDDVRQ
                     NYERISLAAFEASRRWRTTQGTKAAVAAILSDAVPAH"
     gene            5098..5772
                     /locus_tag="AC16HB_15065"
     CDS             5098..5772
                     /locus_tag="AC16HB_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR5608157.1"
                     /translation="MPNATEFLHAMALVPLTLLPIINPLAGAPVFTLTAGDDPMTTRR
                     FARQVAINCWFILVGSLFIGTYVLELFGISLPIVRVGGGLLVAASAWRMLNSGDEDEV
                     RAAVKRSQSAALSRTDVVKRSLFPLTFPLTTGPGTIATAIAIGAGLPRQPALYVVQAL
                     VVMLGATLTVAVIYLVYRNASGLMTRLGEVGSLVMTRLMAFVLLCIGIQILWTGWAEL
                     NGRMNS"
     gene            5836..>6029
                     /locus_tag="AC16HB_15070"
     CDS             5836..>6029
                     /locus_tag="AC16HB_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608158.1"
                     /translation="MKAVEELEREIKRRENELAVFRRALAALKKIKAEGKPVSRRAKR
                     RPLTSAERRKLSESMKKAWQ"
BASE COUNT          977 a         2054 c         2021 g          977 t
ORIGIN      
        1 gtgcgtgccg gtgtgcccca cgggggccag ccacaagcgg cccgaggacg gcatcgtgct
       61 ggtcgactac gacaagtgca tcggctgcaa gtactgcgcg tgggcgtgcc cgtatggcgt
      121 gcgcgaactc gacgcgaagc agcaggtgat gaagaagtgc acgctgtgcg tcgaccgcat
      181 ctacgacgcg aagctgccgg ccgccgagcg caagcccgcg tgcgtgatgg cgtgcccgac
      241 cagcgcgcgc ctgttcggcg acgtgcacga tccagactcg gaggtgtcga tcgcgatccg
      301 cgagcgcgcc ggctacgcgc tgatgcccga atggggcacg cgtccggcca atcactacct
      361 gccgcggcgc aggacggaac tgccgatcgc cgcggaggaa ctgcagcgcg tcgacaaccc
      421 gctgaagatc gacggtaagc tgcccaagcc ggcgaagagc gccccgtcgc tcgacgacgt
      481 gatgtcctgg tgacgccgcc gcgatgcaac cggcattttc cgtcgtcttc ctcaccacgc
      541 tgatcggcgc cggccagggg ctgctgctgg cattgttcgt ggccgagttc ggcgcgcgct
      601 accgctggtg gcttgccgcg cccgcggaca gcggcttctt cgcgctcggc gcggcgatcg
      661 cggtcgcgtt gaccgggctc ggactggtcg cgtccgtgtt ccacctcggc cgccccgagc
      721 gtggctggcg cgcggcggcg cgctggcgca cgtcgtggct gtcgcgcgag gtgatcgtgc
      781 tgcccgcgtt catggcggtc acggtgctgt acggcgccgc gcactggctg ggggccgagc
      841 cggacctgcg cacgctcgcg ctcggcgcgc tcgcggccct gctcgcgctg gcgctgtacc
      901 tgtgcaccgc gatgatctac gcgtgcctga agttcctgca ggaatgggcc acgccgctga
      961 cggtggccaa ctacctgctg ctcggcgcga gttccggctt ctcgctggcg gccgccgtcg
     1021 ccgcgctgcg cgcgccgcaa ctcctcgact tcctcgtcgc gtgggcggtc gcgttgaccg
     1081 cggccgccct cgcctcgcgc gcggcctcgc tgatccgcaa cgcgcggctg aagccgaggt
     1141 ccacgctgca atccgccatt ggcgtcaaac acccgcgcat cgtgcagaag tcgatggggt
     1201 tcatgggcgg ctcgttcaac acccgcgagt tctttcatgg ccgcacgctt tcgctgctgc
     1261 gcgccgtcaa gtggaccttc ctggtactgg tcttcccggt gccggcgctg ctgcttgtga
     1321 cgggcctcac actgtcttcg tcgaccctgc tgatcgcggc gtttctggtt caatatgcag
     1381 gccttctggc cgagcgctgg ttcttcttcg cacaggccaa tcacccgcag aacctctact
     1441 accagcaggt gtcttgagcg gtaatgttgc gctacgtcgg catcggcgcc ggcggatggc
     1501 atacgatgcg tcgatcgccc accggatcgt gaggccgaat tgaacgccag ggatccacag
     1561 tccgaatccg gcacggtcgc cgtcccgaac gcggtgcccc tgacggccga tgccggccgc
     1621 gtgccaccgc attcagtgct gttcgatttc ctcggactgg tcattcgact gaccggcgcg
     1681 cgcgccggcg cgatccgcgc tccgacgccg gacggcgtca gcctgcggct gatggccgca
     1741 gtcggtctgt cggacgaagt ctgcgcgcgc gacaacctga tgccggtgag ctgcggcatg
     1801 tgcggcgccg cgctgcagag cctgctgacg cggcaagcca cgccggcgat cggctgcgcg
     1861 atccacagca gccacgcgac gctgagcgag ttcagcggcc gtgccgtcgc ggtgccgctc
     1921 gatcacctcg acaagccgat cggggtgctg aacctgttct ttgccgaagg ctccgatccg
     1981 ccgccgggta tcgcccacct gctgcggccg ttcgggcaac tgcttgggct ggcactcgag
     2041 aacgagcggc tgacgcgcga aaacctgcgc gtgcggctgg cggccgagcg gcaggcgatg
     2101 gcagccgaca tccacgacgc gctggcgcag tcgctgacct tcgtgcgcat gcgcatgtcg
     2161 ctgctgaacg atgcgatccg agggcaggac tgcaacagcg cgcagaccta ctggaacgac
     2221 gccagcgacg agctgcgcaa tgcacacggt cggctgcgcg agatcatctc gctgtatcgc
     2281 gccggcatgg cttcgcgcgg gctggcggca tcgctgatcg agctggcgtc cgtgttccgg
     2341 cagcgcacgg gcatcgatct gcagttcgac ccgccaggca gcgatttcga gctgacgacc
     2401 gagcagcagt tgcaggtctt ctacatcctc caggaggcgc tggccaacgt cgcgcgccac
     2461 tcgggcgcgc acgaagccag cctgcgcacc gagcgtacgg acgacgaact gcgcttcatc
     2521 gtcgaggacg acgggcgcgg gccgagcgag gagattctcg atccgcgctg cgccggccgg
     2581 ctgaccgagg ccggtcatca cggcctgcag atcatgcgcg agcgcgccgg gcacgccggc
     2641 gggcggctga agatcgaggc gctcccggtc ggcggcaccc gcgtcacgct cgcgatacac
     2701 cgtccacagc gatccacggg agcacgcgcg ctcgcatgaa cccgccgccg atcaggctcg
     2761 tactcgtcga cgatcacgca ctggtgcggc agggactcgc ggatctgctg tcgcgcggcg
     2821 cgggcatgca ggtgctggcg acgacggggc gcgccgaaga gacccgcgcg ctgctcgaga
     2881 agtaccagcc cgacctgctg atcctcgatc tgcggatggg gccgaccgac ggcatcacgg
     2941 tgttgcgcga cctgcgcgag caaggcgtgc agacgccggt gatcgtgctg acgatgagcg
     3001 aggcggccga ggacctcgcc gccgcgctgc ggctgggcgt gcgcggctac ctgctgaagg
     3061 acatggaacc cgccgacgtc atcgagggca tccgccgcgt cgcgcgcggc gaactcgtcg
     3121 tcgcgccgtc gattgccggc aagctgtcga cgatcctgca aagcggcgac gaacccggca
     3181 gcgccgcaag cctgctcaag cagctcacgg gccgcgaacg ccagatcctc ggccacgtgg
     3241 ccgcgggcaa gagcaacaag gcgatcgcca aggcgctcgg catcagccac gacaccgtga
     3301 aactgcacgt gcgccacatc ctcgccaagc tgggcctgag ttcgcgtgtc gaggccgccg
     3361 tgttcgcggt gcagaatcgg gcggtgctgg gcggggacgt gcgctgaagg catgacgtag
     3421 aaagctccta cgcgagccgc gtgaagcgcg gcgcgagtca cacgatccgc ccggcatgtt
     3481 gcctcggaag gtcgtgtcaa agagtcggac gcggactgtt gcgtcgtccc cagatagctt
     3541 cagttcgtca acttctccac tcgcgctccg ctcggcgacc gatgtatcga cagcgtcgcg
     3601 cagagacggg ttctgcagat gttcacctga ggcgggaaac gccgcgcaaa cccggacacc
     3661 gccttcggcc tgcctcagcc cggttcagtg cgccggcacc gcgtcggaca ggatcgcagc
     3721 gacggcagcc ttcgttcctt gagtcgtgcg ccaccgtcgg ctcgcctcga atgccgccag
     3781 cgaaatccgc tcgtagttct gccgcacgtc gtcgatggcg cggaagatcg catcgatcga
     3841 caggacgtcg accgtcttgc cggcgcctgt ttcctcgacg agcaggccgg gcgccgtccc
     3901 cgacggaacg atgaccggta tcccgctcgc gatcgcatcc cagacgatgc cggatccttt
     3961 ccagcgatac gcggccggct cgtacggcac gaccaccacg tcgcatcttg cgatctcctc
     4021 cacgagggag ggcacgtaac ccaggacggt cagtcgcgga gaactctcgg cacgcaccag
     4081 gcccctgctg tcgtgcagaa caacctcgaa tccctcccgg agcagtcgcg atgccagttc
     4141 cgcgagcatg gctgcgccct tctccgggcg ctgagacccg aagaagccaa tccgggtgag
     4201 cccggtcttg caagtgccga tcggcgcgcc gtcgtgtgcc accggaaaac agtcgatccg
     4261 gccttcggcc gtcagcggtg cgtattcctt ctgcagcagc ggacccgtca cgccgacatt
     4321 caaacgaagt ctggcgcgtt gagccatcag gaatgcgtag cgccaccaca tccggccgtt
     4381 gtccgccccg tagctgggct cgacatgaat gcatccggag attctcacct cccgcgcgac
     4441 cttcgagcag gcgaagagct gcggagcaaa gagcgacggc caaagccaaa gatcgcactc
     4501 gccgatgcgc gccagctcct gtgcgagcac ggaactgccg ccgagaaaga cggcgagttc
     4561 ccccgcgacc gggtcgatga gccgggggtc gatgtatgga ttgacggtga acagcggcaa
     4621 cagcttcgcg cggcccgaaa acgactcgac aacctggcga tccgcgcgcg cgtgggcgaa
     4681 cacctcgacc tgatggccga gcgacgtcag ttcgtcgaca accctcaggt cccattcgag
     4741 atgatggccg gccgcggaga tgagcccggg atcgagaaga ctgatgcgct tgctgttcac
     4801 gaatccttat atcaaaccgg gatgcagaga gagcgccgcg cgactcgtct gcgtcgcgat
     4861 gcacgagcca gactgcccga tagttcccgg cgaggcaagt gcaggtgccc tggcccgaac
     4921 tgccgggtcg tctataggca ggcgcagagg aactgccacc gctgagcgga tgggatcgcg
     4981 ccttccccgg acttggtttc aatcgcgcgc ggccatgcct tcgcgtctgc cccctgcacc
     5041 tccgacgtac actgcggccc cacggcgtcg cgcgccgcct cgttgcaccc gcccaccatg
     5101 cccaatgcca ccgaattcct gcacgccatg gcattggtgc cgttgacact gctgccgatc
     5161 atcaacccgc tggccggcgc ccccgtgttc acgctgaccg ccggcgacga cccgatgacc
     5221 acgcgccggt ttgcgcgcca ggtggcgatc aattgctggt tcatcctcgt gggttcgctg
     5281 ttcatcggca cctacgtgct ggagttgttc ggcatttccc tgcccatcgt gcgcgtcggc
     5341 ggcgggctgc tggtggccgc ctcggcatgg cgcatgctga actccggcga cgaggacgag
     5401 gtgcgcgcgg cggtcaagcg ttcgcaaagc gctgcgttgt cgcgcaccga tgtcgtcaag
     5461 cgcagccttt tcccgttgac gtttcccctc accacggggc cgggaacgat cgccaccgcg
     5521 atcgcgatcg gcgccggtct gccgcgccag ccggccctgt acgtggtgca ggcactcgtc
     5581 gtgatgctcg gcgcgacgct gaccgtcgcg gtgatctacc tcgtctatcg caatgcctcg
     5641 gggttgatga cgcggctcgg cgaagttggc agtctggtga tgacccggct gatggccttc
     5701 gtgctgttgt gcatcggcat ccagatcctc tggaccggct gggcggagct gaacggcaga
     5761 atgaacagct gaaggccgtc ggcgaagtgc gttgagtaag atcgatcgca gcaaacgttg
     5821 tgagggaggc aaaccatgaa ggctgtcgaa gaactggaac gcgagatcaa gcggcgcgag
     5881 aacgagcttg cggtcttcag gcgcgcgctt gcggctctga agaagatcaa ggcggaaggc
     5941 aaacccgtct cccgtcgcgc caagcggcgt ccgctcacat cggcggaaag gcgcaagctg
     6001 agcgaatcaa tgaagaaggc ctggcagaa
//
LOCUS       DBNHBY010000627         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_247807, whole genome shotgun sequence.
ACCESSION   DBNHBY010000627 DBNHBY010000000
VERSION     DBNHBY010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_247807"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC16HB_15075"
     CDS             complement(<1..571)
                     /locus_tag="AC16HB_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5608159.1"
                     /translation="MVERRPALDLLAYAILALGVVVVALPVYVTFVASTQTLDQIVKV
                     PMSLAPGTELIHNYSAVLTSGSGRTAAAPVAHMMWVSLIVALVVPAGKIVISLLSAFA
                     FVYFRFPGRMFFFWMIFITLMLPVEVRIVPTYAVIARLDLLNSYAGLIIPLVASATAT
                     FLFRQFFMTIPDELAEAARIDGAGPMRFFR"
     gene            complement(571..1452)
                     /gene="ugpA"
                     /locus_tag="AC16HB_15080"
     CDS             complement(571..1452)
                     /gene="ugpA"
                     /locus_tag="AC16HB_15080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HIR5608160.1"
                     /translation="MEKRVVFRSRWLPYALVAPQLAVTIVFFFWPAAETVYQSLLLQD
                     PFGTSVQFVGLENFRHLYHDESYRASFGVTAIFSVLVASLGLALSLLLAVMADHIVRG
                     ALVFRTLLIWPYAVAPAIAGVLWVFMFSPAIGILPWWLDRIGITWNHLLDSRQAMALV
                     VIAAVWKQISYNFLFFIAGLQSIPKSLIEAAAIDGAGPMRRFRTIVFPLLSPTTFFLL
                     VVNIVFAFFDTFGIIDAATQGGPGQSTTNLVYKVFQDGFKGLDLGSSAAQSVILMAIV
                     IALTVVQFRYIERKVQY"
     gene            complement(1472..2785)
                     /gene="ugpB"
                     /locus_tag="AC16HB_15085"
     CDS             complement(1472..2785)
                     /gene="ugpB"
                     /locus_tag="AC16HB_15085"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HIR5608161.1"
                     /translation="MVRKTMLAALAALAAGALPAAQAQTEVQWWHSMTGVNNDRVNNL
                     AKGFNATQNQCRVNAVFKGSYDDSMTAAIAAFRAGTAPNILQVYDVGTATMMYSKAIK
                     PVAEVMRESGAAWNPNAYVPAVASYYTSPKGDVVSFPFNSSTTVFFWNKDAFKAAGLD
                     PGKPPKTWTDVAAAGAKLKAAGHKCPLTMSWQTWTQLESFSAWHNTEFATKNNGFGGL
                     DARLLINGPLFVRHIENLANLAKQGIFVYGGRGAAGDAKFSSGECAMTMASSAIYGLT
                     KSTLKAEFGISTLPYYADVSGAPQNTVIGGASLWVLAGKKKEENQCVGRFFAYLSQPE
                     IQATWHQDTGYLPLTLAAYELTRKSGFYDKDPGTDVSVQQMIVKTTDKSRGLRLGNMV
                     QIRAIMEEELENVWGGKKTPKQALDDAVRRGNEQLERFQRVSQGR"
BASE COUNT          532 a         1024 c         1012 g          522 t
ORIGIN      
        1 cccggaagaa gcgcatcggc ccggcgccat cgatgcgcgc cgcctcggcc agttcgtccg
       61 gaatcgtcat gaagaactgg cgaaacagga atgtcgcggt ggcggacgcg acgagcggga
      121 tgatcaggcc ggcgtagctg ttcagcaggt cgagccgcgc gatcaccgcg taggtcggca
      181 cgatccggac ctcgaccggc agcatcagcg tgatgaagat catccagaag aagaacatcc
      241 ggccgggaaa acggaagtac acgaacgcga acgcggacag cagcgagatc acgatcttgc
      301 ccgccggcac cacgagcgcc acgatcaggc tgacccacat catgtgcgcg accggcgcgg
      361 ccgcagtgcg acccgagccg gacgtcagca ccgcgctgta gttgtggatc agctcggtgc
      421 ccggcgcgag ggacatcgga accttcacga tctggtcgag cgtctgcgtc gacgcgacga
      481 aggtgacgta caccggcagc gcgaccacga ccacgccgag cgcgaggatc gcgtacgcga
      541 gcaggtccag ggccgggcgt cgctcgacca tcagtactgc accttgcgct cgatatacct
      601 gaactgcacg accgtgagcg cgatcacgat cgccatcagg atcaccgact gcgcggcgga
      661 actgccgagg tcgagcccct tgaagccgtc ctggaatacc ttgtagacga ggttcgtggt
      721 cgactgcccc gggccgccct gcgtggccgc gtcgatgatg ccgaacgtgt cgaagaacgc
      781 gaacacgatg ttgaccacga gcaggaagaa cgtggtcggc gacagcagcg gaaacacgat
      841 ggtccggaag cgccgcatcg gcccggcgcc atcgatggcc gccgcctcga tcagcgattt
      901 cgggatcgac tgcagcccgg cgatgaagaa caggaagttg taggaaatct gcttccacac
      961 cgcggcgatc acgaccagcg ccatcgcctg ccggctgtcg agcaggtggt tccaggtgat
     1021 gccgatgcgg tcgagccacc acggcaggat gccgatggcc gggctgaaca tgaacaccca
     1081 cagcaccccg gcgatcgccg gcgccaccgc atacggccag atcagcaggg tgcggaagac
     1141 cagcgcgccg cgcacgatgt ggtcggccat cacggccagc agcagcgaca gcgccagccc
     1201 cagcgatgcc accagcaccg agaagatcgc ggtgacgccg aacgacgcgc gatagctttc
     1261 gtcgtgatac aggtggcgga agttctccag cccgacgaac tgaacgctgg tgccgaacgg
     1321 atcctgcagc agcaacgact ggtagaccgt ctccgcggcc ggccagaaga agaagacgat
     1381 cgtgaccgcg agctgtggcg cgaccagcgc gtacggcagc cagcgcgagc ggaagacgac
     1441 gcgcttttcc aaggtcgtcg tccgggcgcc gctaccggcc ctgactcact ctctggaacc
     1501 gctcgagctg ttcgttgccg cgccggaccg cgtcgtccag cgcctgcttc ggcgtcttct
     1561 tgccgcccca gacgttctcg agttcctctt ccatgatggc gcggatctgc accatgttcc
     1621 cgagccgcag accgcgggac ttgtccgtcg tcttgacgat catctgctgc accgacacat
     1681 cggtgccggg atccttgtcg tagaagccgg acttcctggt cagctcatag gcggcgagcg
     1741 tgagcggcag gtagccggtg tcctggtgcc aggtcgcctg gatctcgggc tgcgacaggt
     1801 aggcgaagaa gcggccaacg cactggttct cctccttctt cttgcctgcc aggacccaca
     1861 ggctcgcgcc gccgatcacc gtgttctgcg gcgcgccgga tacgtccgcg taatacggca
     1921 gcgtcgagat gccgaattcg gccttcagcg tgctcttggt aagaccgtag atcgccgacg
     1981 aggccatcgt catcgcgcac tccccgctgg agaacttggc gtcgcccgcg gcgccgcgcc
     2041 cgccgtagac gaagatcccc tgcttggcga ggttggcgag gttctcgatg tggcgcacga
     2101 acagcgggcc gttgatgagc aggcgcgcgt cgagcccgcc gaagccgttg ttcttcgtcg
     2161 cgaactcggt gttgtgccag gcgctgaagc tctcgagctg cgtccacgtc tgccacgaca
     2221 tcgtcagcgg gcacttgtgg cctgcggcct tcagcttcgc gccggccgcc gcgacgtcgg
     2281 tccatgtctt cggcggcttg cccggatcga gtcccgccgc cttgaacgcg tccttgttcc
     2341 agaagaacac ggtggtcgag ctgttgaacg ggaacgacac cacatcgccc ttgggcgacg
     2401 tgtaatagct cgcgaccgcg ggcacgtacg cgttcgggtt ccaggccgcg ccgctttcgc
     2461 gcatcacctc ggcgaccggc ttgatcgcct tcgagtacat cattgtcgcg gtgccgacgt
     2521 cgtacacctg caggatgttc ggtgcggtgc cggcgcggaa tgccgcgatt gccgccgtca
     2581 tcgagtcgtc gtagctgccc ttgaacaccg cgttgaccct gcattggttc tgggtggcgt
     2641 tgaaaccctt ggccaggttg ttgacgcggt cgttgttcac gccggtcatc gagtgccacc
     2701 actgcacttc ggtctgtgcc tgggcggcgg gcagggcgcc ggcggcaagc gcggcgagcg
     2761 ccgcaagcat cgtcttgcgt accatcgtga tctcctggtt ggaccggatt gttctttacg
     2821 ttgtctgcgc cgcggcgtgt tgacccgcgg caatgggcgg cggcgattgt cgcgcgtagt
     2881 gcagcgcgcg gacaacccgc gagcgcgccg cgcggaccgt cacttgcggg tacctcgagg
     2941 ctgttagcat gtcccgccgg ctgcctcgca cagcggtcga tcctgtcacc gacgttctcc
     3001 cctccgctcg agtctcccgt gctgcgcgac cgtctgatcc tgtacgcgcg tctgactcgg
     3061 ctcgacaagc cggtcggcac gctgctgctg
//
LOCUS       DBNHBY010000628         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1464710, whole genome shotgun sequence.
ACCESSION   DBNHBY010000628 DBNHBY010000000
VERSION     DBNHBY010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1464710"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16HB_15090"
     CDS             complement(<1..1007)
                     /locus_tag="AC16HB_15090"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5608162.1"
                     /translation="MKQFDWINPYPTVRTPVFARNLVSTSQPLAAQAGLRTLQRGGNA
                     VDAAIAAAAVITMTEPCSNGLGSDNFAIVWDPASKQLHGMNSSGIAPAAWTLDYFRRK
                     YGDDLPKNRPQRGWDTVTIPGAVAGWSLLHGRFGKLPFADVLAPAIDYAEHGFAVSPI
                     VQDKWQRAASILEPYPGFAAHFLPRGRAPHIGEHFVLQGAARTLKLVAQTKGEAFYRG
                     EIAAAIAKYARESGGAHTEADFAAYWDFVQKNGWVGTVNQSIAGFEIHEIPPNGQGIA
                     ALICLGILKHTPLAQQAFDRGRFDSPDVQHVLIEAMKLAFADVYTYVADPRAMRVTPA
                     Q"
     gene            complement(1004..1606)
                     /locus_tag="AC16HB_15095"
     CDS             complement(1004..1606)
                     /locus_tag="AC16HB_15095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5608163.1"
                     /translation="MIVLLFEVTPTAGAQDEYLDIAAKLRPALDALGGCLFLDRFRSL
                     ARRDTLLSFQLWRDDAALRRWRADPQHRSAQTLGRTRIFADYRLRIARVVRAESRGEP
                     AWRPQRHDAHDDPAVRAPRYVAIVESQSGVMAGAAESFASIYRAGAYAHVFDIAAPDA
                     LTLAQLPDAQQLRVGRIERDYGMYERAEAPQFYSPMERVS"
     gene            1722..2009
                     /locus_tag="AC16HB_15100"
     CDS             1722..2009
                     /locus_tag="AC16HB_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608164.1"
                     /translation="MARFLRFIAIRSRRLARLPTVRSNAARAARQNSKDRTMKPTSIK
                     TKIAGLFFALLMSAAVLGATVAGMLAGSQASGDVVSLDRTVVLAPSATAIN"
BASE COUNT          332 a          670 c          682 g          325 t
ORIGIN      
        1 atctgcgcgg gcgtcacacg cattgcacgc ggatcggcca cgtaggtgta gacatccgca
       61 aaggcgagct tcatcgcctc gatcagcacg tgctgcacgt cgggcgaatc gaagcggccg
      121 cggtcgaacg cctgctgcgc gagcggcgtg tgcttaagta tcccgaggca gatcagcgcg
      181 gcgatgccct gaccgttggg cggaatctcg tgtatctcga agccggcgat cgactggttc
      241 acggtgccga cccagccgtt cttctgcacg aagtcccagt acgcggcgaa gtcggcttcg
      301 gtgtgcgcgc cgccggattc acgcgcgtac tttgcgatcg cggcggcgat ctcgccgcga
      361 taaaacgcct cgcccttcgt ctgcgcgacg agcttcagcg tgcgcgccgc gccttgcagc
      421 acgaaatgct cgccgatatg cggcgcccgc ccgcgcggca ggaagtgcgc ggcgaacccc
      481 ggatagggct ccaggatcga agcggcgcgc tgccacttgt cctgcacgat ggggctgacc
      541 gcgaagccgt gctccgcgta gtcgatcgca ggcgcgagta cgtctgcgaa cggcagcttg
      601 ccgaagcggc cgtgcagcag cgaccagccc gccaccgcgc cgggaatcgt caccgtgtcc
      661 cagccgcgct gtgggcggtt cttcggcagg tcgtcaccgt acttgcgccg gaagtagtcg
      721 agcgtccagg cagccggcgc gatgccgctc gagttcatgc cgtgcagttg cttcgatgcg
      781 gggtcccaca cgatcgcgaa gttgtccgag cccagcccgt tgctgcacgg ctcggtcatc
      841 gtgatcaccg cggcggcggc gatcgccgcg tcgaccgcgt tgccgccgcg ttgcagcgtc
      901 cgcagtccgg cctgggcggc cagcggctgc gaggtcgaca cgagattgcg cgcgaacact
      961 ggcgtgcgca cggtcggata cggattgatc cagtcgaact gcttcacgaa actctctcca
     1021 tcggtgaata gaactgcggt gcctcggcgc gctcgtacat gccgtagtcg cgctcgatcc
     1081 tgccgacgcg cagttgttgc gcgtcgggaa gttgcgccag cgtcagcgca tcgggcgcag
     1141 cgatgtcgaa cacgtgcgcg tacgcgccag cgcggtagat gctcgcgaac gattccgccg
     1201 cgccggccat cacgcccgac tgcgattcga cgatggccac gtagcgcggc gcgcgcacgg
     1261 ccggatcgtc atgcgcgtcg tgccgttgcg gtcgccacgc gggctcgccg cgcgactctg
     1321 cgcgcacgac ccgcgcgatg cgcagccggt agtcggcaaa aatgcgcgtg cggccgagcg
     1381 tctgcgcgct gcggtgctgc ggatcggcgc gccagcgcct cagcgccgca tcatcgcgcc
     1441 agagctggaa cgacagcagc gtgtcgcgcc gggccaggct gcggaaacgg tcgaggaaca
     1501 ggcagccgcc gagtgcatcg agcgcgggcc gcagcttcgc ggcgatatcc agatactcgt
     1561 cctgtgcgcc ggctgtcggc gtgacttcga acagcaggac gatcatcggg atcgatgggc
     1621 gagatgaagg aatgaacggc ggatacgcac gacgaaacgc cgaaaagcgg gaatgagttg
     1681 ctcattctgc cagtggcggc gcgaaacgac gcttccgctt catggcgcga tttctgcgct
     1741 tcatcgcaat tcgctcgcgg cgcctggcgc ggctgcctac agttcgatcc aacgcagccc
     1801 gcgctgcccg acaaaactcg aaggaccgaa ccatgaaacc gacatcgatc aagacgaaaa
     1861 tcgcaggcct gttcttcgcg ctgctcatga gcgcggcggt gctcggcgcg accgtcgccg
     1921 gcatgcttgc gggtagtcaa gcatcgggcg acgtggtctc cctcgatcgc acggtcgtcc
     1981 tcgcgccgtc ggcgacggcg atcaactag
//
LOCUS       DBNHBY010000629         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1058711, whole genome shotgun sequence.
ACCESSION   DBNHBY010000629 DBNHBY010000000
VERSION     DBNHBY010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1058711"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC16HB_15105"
     CDS             <1..204
                     /locus_tag="AC16HB_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608165.1"
                     /translation="FAAALSGVKRALVIEQNHGAQLYRYLRSQYDLPRTASFHRPGPL
                     PLRPGEVANAIVEWANAKEEVPA"
     gene            201..1037
                     /locus_tag="AC16HB_15110"
     CDS             201..1037
                     /locus_tag="AC16HB_15110"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR5608166.1"
                     /translation="MNDIAPAPLTAASYKSNYKPIWCPGCGDYTVLSSITKALAMLQL
                     LPHQVAVVSGIGCSSRIPAYTSCYGFHGVHGRALAAATGLKVARPDLTVLVAGGDGDG
                     YSIGGNHFLHACRRNVDLTYIVMDNHVYGMTKGQASPTTEPDWDNKLSPGGTGIRAFH
                     PLVIALASGANFVARAFSGDPNGTANIIAAAVRHPGFSFVEILSPCVTFRPEQRDWKG
                     MVHAAPVEPTDDAARAARRIMTDDGFNIGVLYAGNRATYPHYRSGEAKCTVTDLEAEF
                     EQ"
     gene            1034..1543
                     /locus_tag="AC16HB_15115"
     CDS             1034..1543
                     /locus_tag="AC16HB_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR5608167.1"
                     /translation="MSAPQNLEEFMALALAMEREAAERYTEFADAMDTHNNREVAAMF
                     RTMAGYENKHAQQIMAEMGWKEAPPLRATLTWPDFEAPETVAIDEVHYLMQPWHALQL
                     ALAAEQRAERFFAELARVATTDSVRRAALELQAEEAEHVALVKQWIDKVPQPDRNWAE
                     DPDPPRYSD"
     gene            1584..1976
                     /locus_tag="AC16HB_15120"
     CDS             1584..1976
                     /locus_tag="AC16HB_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5608168.1"
                     /translation="MQVLLPEGWAPPIGYSNGIAVDAGRIVFIAGQVGWDAQQKFHSE
                     EIAPQFEQALRNVLAVLAQAGGRAEHICRITAYCCDKRAYLAARKDLGAIWRRHMGKH
                     FPAMSMIFVSDLLDSPGKIELEATAVVP"
BASE COUNT          360 a          710 c          716 g          290 t
ORIGIN      
        1 ttcgccgccg cgctgtccgg cgtgaagcgc gcgctggtga tcgagcagaa ccacggcgcg
       61 cagctctatc gctacctgcg cagccagtac gacctgccgc gcaccgcgag cttccaccgg
      121 ccgggaccgc tgccgttgcg gccgggcgag gtggcgaacg cgatcgtcga atgggccaac
      181 gcgaaggaag aggtaccggc atgaacgaca tcgcgcccgc gccgctgacc gcggccagct
      241 ataagtcgaa ctacaagccg atctggtgtc cgggatgcgg cgactacacc gtgctgtcgt
      301 cgatcaccaa ggcgctcgcg atgctgcaac tgctgccgca tcaggtcgcg gtggtctcgg
      361 gcatcggctg ctcgtcgcgc atccccgcgt acacctcgtg ctacggcttt cacggggtgc
      421 acggccgcgc actcgccgcc gccacggggc tgaaggttgc gcggcccgac ctgacggtgc
      481 tcgtggccgg cggcgacggc gacggctact cgatcggcgg caaccacttc ctgcacgcgt
      541 gccgccgcaa tgtcgatctg acctacatcg tgatggacaa ccacgtgtac ggcatgacca
      601 aggggcaggc atcgccgacg accgagcccg actgggacaa caagctctcg cccggcggca
      661 ccggcatccg cgcgttccat ccgctggtga tcgcgttggc ctccggcgcc aacttcgtcg
      721 cgcgcgcgtt ttcgggcgat ccgaacggca ccgcgaacat catcgcggcg gcggtccgtc
      781 atccgggctt ttcgttcgtc gagatcctga gcccctgcgt gaccttccgg cccgagcagc
      841 gcgactggaa gggcatggtc catgcggcgc cggtcgagcc gaccgacgat gcggcgcgcg
      901 ccgcgcgccg catcatgacc gacgacggct tcaacatcgg tgtgctgtac gccggcaacc
      961 gcgcgacgta cccgcactac cgcagcggcg aagccaagtg caccgtcacc gacctggaag
     1021 cggagttcga gcaatgagcg caccgcagaa cctcgaagaa ttcatggcgc tggcgctggc
     1081 gatggagcgc gaagcggcgg agcgctacac cgagttcgcc gacgcgatgg acacgcacaa
     1141 caaccgcgag gtggcggcga tgttccgcac gatggcgggc tacgagaaca agcacgcgca
     1201 gcagatcatg gccgagatgg gctggaagga agcgccgccg ctgcgcgcca cgctgacctg
     1261 gcccgacttc gaagcgccgg agacggtcgc gatcgacgaa gtgcactacc tgatgcagcc
     1321 gtggcacgcg ctgcagctgg cgctcgccgc cgagcagcgc gccgagcgtt tcttcgccga
     1381 gctcgcgcgc gtggcgacca ccgactcggt gcggcgcgcc gcgctggaac tgcaggccga
     1441 ggaggccgag cacgtggcac tcgtcaagca atggatcgac aaggtgccgc agcccgaccg
     1501 caactgggcc gaggatcccg atccgccgcg atattcggac tgagatgcgc tgagtgacag
     1561 gcacacgctc gagaggagac gacatgcagg ttctgctgcc ggaagggtgg gcgccgccga
     1621 tcggctattc gaacggcatc gcggtcgacg ccgggcgcat cgtgttcatc gccggccagg
     1681 tcggctggga tgcacagcag aagttccact ccgaggagat cgcgccgcag ttcgagcagg
     1741 cgcttcggaa cgtcctcgcg gtgctcgcgc aggcgggcgg ccgagccgag cacatctgcc
     1801 gcatcacggc ctactgctgc gacaagcggg cctacctggc cgcgcgcaag gacctcggcg
     1861 cgatctggcg caggcacatg ggcaagcact tcccggcgat gagcatgatc ttcgtatccg
     1921 acctgctcga cagcccgggc aagatcgagc tggaggcgac cgcggtggtg ccctgaaccg
     1981 gcgccgtcag tgcgcttccc gcggcgtgac ggtgaagtcc agcatgcggc cgcgacggaa
     2041 cgcgcgaacg gcaatcggcc gctcgatgcg ggaggc
//
LOCUS       DBNHBY010000630         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_247974, whole genome shotgun sequence.
ACCESSION   DBNHBY010000630 DBNHBY010000000
VERSION     DBNHBY010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_247974"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="AC16HB_15125"
     CDS             <1..1005
                     /locus_tag="AC16HB_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5608169.1"
                     /translation="LNVISAQVTVRPSGHQFLLEGNDSILQAGLKAGMRFAYGCGTGT
                     CGLCKARVVSGEVRQIEHSDYPLSEAEKRQGYALLCTCTAVTDVVVETIEARGPADIP
                     AQELVATVRAIEPLAPDTLLLHLQTPRSNRLRFLAGQSVTLGLPSEQGDLTQSIALAS
                     CPCDERNLHFHFARDVEAPLAQALFESRIRPGAPISVRGPSGDFVLDPDSTRPLMLVA
                     CDTGFGPIKSLIEHALAGESTEAFDLIWLATRGDGHYLANQCRAWAAAFDTFSFRLLT
                     DASAAAGAWQAIEAVTASRRELAQCDVYAAGPSEFVEPVLEGLRGAGALPGRMHGLVT
                     "
     gene            1015..1641
                     /locus_tag="AC16HB_15130"
     CDS             1015..1641
                     /locus_tag="AC16HB_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR5608170.1"
                     /translation="MSDALNFLVKARPDAMGHYFKFLKEAGKHLDPRTRNLISVITKV
                     HAQTERGLRQYLSRALREGCTPMEVLDALLMAFPALGLAKIVWAVDIIIDMDLPDFQP
                     DAMGLPGAWHDVMAAEALAVGGVERVECDGRGLFVYRGDAEFRVYDSRCPHQTTNIPH
                     LALQGTRLTCPKHEWAFDITTGACVAKGSTPLKRWKSKVEGGRLFAYW"
     gene            1643..3046
                     /gene="cysG"
                     /locus_tag="AC16HB_15135"
     CDS             1643..3046
                     /gene="cysG"
                     /locus_tag="AC16HB_15135"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HIR5608171.1"
                     /translation="MKTLPIFLKLAGQPCAVIGGGEVAARKASLLLDAGARVTVTAPS
                     LCGALTELAQQGRITHRAERFSAAAIEDVAVVIAATDDRAVNAEVSRLAQARRIPVNV
                     VDDPELCSFILPAIVDRSPVVIAVSTGGASPVLARLLRARLETMIPATYGQLATLVQR
                     FRERVKTRLPQAARRPFWEQVLQGPVAELVFSGRAAQAEQALERAVREASDAGLPRGE
                     VYLVGAGPGNPELLTLAALRLMQQADVVLHDNLVSPAILELTRRDAQRIYVGKQRADH
                     AMRQEHINELMVELARQGKRVLRLKGGDPYVFGRGGEEIETLAASGIRFQVVPGVTAA
                     CGVAAYAGIPLTHRDYAQSCVLVTGHLKDGSMNLDWHGLARPRQTVVVYMGLLGLQKL
                     ADELIAHGMPPDMPAAVVQQATLPQQRVVTAALAELPQRVEEAGLHPPTLVIVGDVVK
                     LREQLDWYDAGRVTAAG"
BASE COUNT          469 a         1060 c         1153 g          462 t
ORIGIN      
        1 ttgaacgtga tctcggcgca ggtgacggtg cggccgagcg ggcatcagtt cctgctcgaa
       61 ggcaacgact cgatcctgca ggccgggctg aaagccggca tgcgcttcgc ctatggctgc
      121 ggcaccggca cctgcgggct gtgcaaggcg cgcgtggtga gcggcgaggt gcggcagatc
      181 gagcactccg actatccgct gtcggaggcg gagaagcggc agggctacgc gctgctgtgc
      241 acgtgcaccg ccgtcaccga cgtggtcgtg gagacgatcg aggcgcgcgg tccggccgac
      301 attccagcgc aggaactcgt tgccacggtg cgcgcgatcg aaccgctcgc gcccgacaca
      361 ctgctgctgc acctgcagac gccgcgcagc aaccggttgc ggtttctggc cggccagtcg
      421 gtcacgctcg gcctgccgag cgagcagggc gacctgacgc agtcgatcgc gctggcaagc
      481 tgtccgtgcg acgagcgcaa tttgcacttc cacttcgcgc gcgacgtcga agcgccgctg
      541 gcgcaggcgc tgtttgaaag ccgcatccga cccggcgcgc cgatcagcgt gcgcgggccg
      601 agcggcgact tcgtgctcga ccccgacagc acgcggccgc tgatgctcgt cgcctgcgat
      661 accgggttcg gtccgatcaa gagcctgatc gagcacgcgc tggcaggcga gagcaccgag
      721 gcgttcgacc tcatctggct cgccacgcgc ggcgacgggc actatctggc caaccaatgc
      781 cgcgcgtggg cggcggcgtt cgacacgttc tcgttccggc tgctgacgga tgcatcggct
      841 gcggccggcg cctggcaggc gatcgaggcg gtgaccgcct cacggcgtga actcgcgcaa
      901 tgcgacgtgt acgcggcggg gccgagcgag ttcgtcgagc ccgtgctcga aggcctgcgc
      961 ggcgccggtg cgctgccggg gcggatgcac ggcctcgtga cgtagagtgg aaccatgagc
     1021 gacgcactga acttcctcgt caaggcgcgg cccgacgcga tgggccatta cttcaagttc
     1081 ctgaaggagg ccggcaagca cctcgatccc aggacgcgca acctgatctc ggtgatcacc
     1141 aaggtgcacg cgcagaccga acgcgggctg cgccagtatc tgtcgcgtgc cctgcgcgag
     1201 ggatgtacgc cgatggaagt gctcgacgcg ctgctgatgg cgttcccggc gctcggcctg
     1261 gcgaagatcg tgtgggcggt cgacatcatc atcgacatgg acctgccgga cttccagccc
     1321 gacgcgatgg gattgcccgg cgcatggcac gacgtgatgg ctgccgaggc gctcgccgtc
     1381 ggcggcgtcg agcgcgtcga gtgcgacggg cgcgggctct tcgtctaccg cggcgatgcg
     1441 gaattccgcg tgtacgacag ccgctgtccg caccagacca cgaacatccc gcacctcgcg
     1501 ctgcagggca cgcgcctcac ctgtcccaag cacgaatggg cgttcgatat cacgaccggc
     1561 gcgtgcgtcg cgaagggctc cacgccgctg aagcgctgga agtcaaaggt cgagggcggg
     1621 cggctgttcg cctactggtg agatgaaaac gctgccgatc ttcctgaagc tcgccggcca
     1681 gccgtgcgcg gtgatcggcg gcggcgaggt cgccgcacgc aaggcgtctc tgctgctcga
     1741 tgcgggcgcg cgcgtcaccg tgacggcgcc ttcgctgtgc ggcgcactga cggaactcgc
     1801 gcagcagggg cggatcacgc atcgcgcaga aaggttcagc gccgcggcga tcgaggacgt
     1861 cgccgtcgtg atcgccgcca ccgacgatcg cgcggtcaac gccgaggtgt cgcggctcgc
     1921 gcaggcacgg cgcatcccgg tcaacgtggt cgacgacccg gaactgtgct cgttcatcct
     1981 gccggcgatc gtcgatcgtt cgccggtcgt gatcgcggtg tcgaccggcg gcgcatctcc
     2041 cgtgctggcg cgcctgctgc gcgccaggct cgagacgatg atcccggcga cctacggcca
     2101 gctcgccacg ctggtgcagc gcttccgcga gcgggtgaaa acgcgcctgc cgcaggccgc
     2161 gcgacggccg ttctgggagc aggtgctgca ggggccggtt gccgagctgg tgttctcggg
     2221 ccgcgcggcg caggccgagc aggcgctgga gcgtgccgtg cgcgaggcat ccgacgcggg
     2281 cctgccgcgc ggcgaggtgt atctcgtcgg tgcggggccg gggaatccgg aactgctcac
     2341 gctcgccgcg ctgcggctga tgcagcaggc cgacgtcgtg ctgcacgaca acctggtgtc
     2401 gccggccatc ctggagctga cgcggcgcga cgcgcagcgg atctacgtcg gcaagcagcg
     2461 cgcggatcac gcgatgcgcc aggagcacat caacgagttg atggtcgaac tcgcgcggca
     2521 gggcaagcgc gtgctgcggc tgaagggggg cgatccgtac gtcttcggcc gcggcggcga
     2581 ggagatcgag acgctcgcgg ccagcggcat ccgcttccag gtcgtgcccg gcgtcaccgc
     2641 ggcgtgcggc gtggctgcct atgcgggcat cccgctcacg catcgcgact acgcgcagtc
     2701 gtgcgtgctc gtgacggggc acctgaagga cggctcgatg aacctcgact ggcacgggct
     2761 cgcgcggccg cgccagacgg tcgtcgtcta catgggactg ctcggcctgc agaagctcgc
     2821 cgacgaactg atcgcgcacg gcatgccgcc cgacatgccg gcggcggtcg tgcagcaggc
     2881 cacgctgccg cagcagcgcg tggtcacggc tgcgctggcc gagttgccgc agcgggtgga
     2941 ggaagccggc ctgcatccac cgacgctggt gatcgtcggc gacgtggtca agctgcgcga
     3001 gcaactcgac tggtacgacg ccgggcgcgt gactgcggcg ggttgatcgt cagtgcgcgc
     3061 cggacaggcg ccgcgtcgca tcggtcagcg ccgctgcgcc accgccacgc caggccgcgc
     3121 agaggtcgcg cgcggcagcg agcg
//
LOCUS       DBNHBY010000631         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_111714, whole genome shotgun sequence.
ACCESSION   DBNHBY010000631 DBNHBY010000000
VERSION     DBNHBY010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_111714"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1255
                     /locus_tag="AC16HB_15140"
     CDS             329..1255
                     /locus_tag="AC16HB_15140"
                     /inference="COORDINATES: protein motif:HMM:NF039473.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HIR5608172.1"
                     /translation="MHVPVHIRAAARRVAWSLKAAPSRADVLLREADTMLAPLARLLV
                     AHGVTYPRLAQALKAVFLQAAQAELAAAGRTPTDSALSLLSGVHRKDVRALARGAART
                     GDNGVRALSFGAEVFTRWANDRRYADAQGRPAALPLHGRGARTFDRLVRSVSKDFHPR
                     SVLDELLRLGVAEVDGERVRLKTRGFVPQQGFREIAYYVAANVRDHLAAADANLRAAG
                     GVEDAPFLEHAVYADEMSPRSAAQLQRLARRVWAAAVKRMYQAANAAVEADRALPDAQ
                     RTARMRFGAFYFAEPGAAPAPVAGRTRRRSSK"
     gene            1252..>1770
                     /locus_tag="AC16HB_15145"
     CDS             1252..>1770
                     /locus_tag="AC16HB_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608173.1"
                     /translation="MIAHAVRWFTALAAAATAAACGGGLIAGVGSGGSGFGVASGIVT
                     GFGSIIVDGTPWDVRDARIEALVDPAQPAALTEAKLGQHVEIDFQTPGVAKTVRVEAN
                     VVGRVSAVDAAATPPRLVAAGQTVRINTDPAGGPVTVFVGYASIGDVQTGDAVEIHGS
                     SEFDATLAANVIQ"
BASE COUNT          241 a          625 c          636 g          268 t
ORIGIN      
        1 tcgtaaccgc acgccggcgc gtcggtcagt gaaacccgca gcgtgcccat cccttgcgcg
       61 gtcgacgtgc cgccggcacc gccaccgcac gcggcgagcg tcagcgcagc gccgctggcc
      121 gcggcaatct gggcaattcg gacaaacatg gcattcatgg cacctcctcg gtgtcggacc
      181 gggtccgcat tgatttatgg tatgggaaat tttcccactt caattacggc gcggtcaaac
      241 tcacccgcgg attactccat ttgtggcatt gtcggccggc cagcgcgcgg gccggcttgc
      301 ggcgcgggcc ggcatgggaa acaatcccat gcatgttcct gttcatatcc gcgcggccgc
      361 ccgccgcgtg gcctggtcct tgaaggccgc gccgtcgcgg gccgacgtcc tgctgcgcga
      421 ggccgacacg atgctggcgc cgctggcgcg cctgctcgtc gcccacggcg tcacctatcc
      481 gcggctggcg caggcgctta aggcggtctt cctgcaggcc gcgcaggccg agcttgccgc
      541 tgcgggccgg acgccaaccg actcagcgct gagcctgctg tcgggcgtgc accgcaagga
      601 cgtgcgcgcg ctggcgcgtg gcgctgcgcg cacgggcgac aacggcgtgc gcgcgctgtc
      661 gttcggggcc gaggtcttca cccgctgggc caatgaccgc cgctacgcgg acgcgcaggg
      721 ccggccggcg gcgctgccgc tgcacggccg cggcgcgcgc accttcgacc gcctggttcg
      781 ttcggtgtcg aaggatttcc acccgcgctc cgtgctcgac gaattgctgc gcctcggcgt
      841 ggccgaggtc gacggcgagc gggtacggct gaagacgcgc ggcttcgtgc cgcagcaggg
      901 tttccgcgag atcgcgtatt acgtcgccgc caacgtgcgc gaccatcttg ccgcggccga
      961 cgccaacctg cgcgcggccg gcggcgtcga agacgcgccg tttctcgagc acgcggtgta
     1021 tgcggacgaa atgtcgccgc gctcggcggc acagttgcag cgtctcgcgc gccgggtctg
     1081 ggccgccgcc gtcaagcgca tgtaccaggc ggcaaacgcg gcggtcgagg ccgaccgggc
     1141 cctgccggac gcgcagcgca cggcccgcat gcgcttcggc gcgttctact ttgcggagcc
     1201 cggcgctgcc ccggcgcctg tcgccggacg cacacgacga aggagttcga aatgatcgcc
     1261 cacgctgttc gatggttcac cgcgctggcc gcggccgcga ccgcggccgc ctgcggtggc
     1321 ggactgatcg ccggggtcgg cagcggcggc agcggcttcg gcgtcgcgtc ggggatcgtc
     1381 acgggattcg ggtcgatcat cgtcgacggc acgccgtggg atgtccgcga cgcgcgcatc
     1441 gaagcgctgg tcgatccggc gcaaccggca gcgctcaccg aggccaagct gggccagcat
     1501 gtcgaaatcg acttccagac gccgggcgtc gcgaagacgg tccgggtcga ggccaacgtc
     1561 gtcggccgcg tcagcgcggt cgatgccgcg gcgacgccgc cgcgtctcgt cgcggcggga
     1621 caaacggtgc gcatcaacac ggatccggcc ggcgggccgg tcacggtgtt cgtcggctat
     1681 gcatcgattg gcgacgtgca gaccggcgat gcggtcgaga ttcacggcag cagcgagttc
     1741 gacgccacgc ttgccgccaa cgtgatccag
//
LOCUS       DBNHBY010000632         6431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_992192, whole genome shotgun sequence.
ACCESSION   DBNHBY010000632 DBNHBY010000000
VERSION     DBNHBY010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_992192"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="AC16HB_15150"
     CDS             complement(<1..735)
                     /locus_tag="AC16HB_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5608174.1"
                     /translation="MSFTTHPLSIRIAAWIAGRDGIEHGDVLLDRRRVYIVPTRAGLV
                     FGVAMLVLLIGSINYALQLGFMLTFLVTSIALVAMYHTHRNLTRVQLRGHGVENVFAG
                     DVATFEIVATNPTAEPRHALGFALLSPARARVASVHVDIPAGSARTVRLGLPAARRGR
                     LACPRIRIETRFPFGLWQAWAYYTPPLTAIVYPNPEDDAPPLPVAAGGEGEGVGLAST
                     GDDFAGVRPYQPGDPQKMIAWRLAARS"
     gene            complement(732..1655)
                     /locus_tag="AC16HB_15155"
     CDS             complement(732..1655)
                     /locus_tag="AC16HB_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5608175.1"
                     /translation="MLDQLRRCAAQISGVIVGKDLQVRQSLTCLLAGGHLLIEDAPGV
                     GKTTLAHAIALSLGLPFRRIQFTSDLLPSDVIGVSVYDRTNAQFVFHPGPIFTEVLLA
                     DEINRASPKTQSALLEAMEESQVSVDGLSRPLPQPFFVIATQNPLHQVGTFPLPESQL
                     DRFLMCISLGYPDRAAERALLAGQSRRDMLRTLSAVARPADLLAAQAEAKKVFVSNAL
                     LDYVQALLAHTRASGKYAEGMSPRAGLALLAAARAWAWFDGREHVIPEDVQNVLPAVA
                     GHRLHVTQREGGRVLHGRELVAQLAQAVAVP"
     gene            1694..2617
                     /locus_tag="AC16HB_15160"
     CDS             1694..2617
                     /locus_tag="AC16HB_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR5608176.1"
                     /translation="MHTGFFTHHVCAAHEMGSWHPESPERLAAIQDHLIATGLAPHIE
                     YVDAPPATLDQVARAHDREYIDALRSRIPKHGYHPLDPDTSMNPSTWEAALRSAGAVI
                     AATDQVVGGGLDNAFCAVRPPGHHARPGAAMGFCFFNNVAVGALHALQHHGLKRVAVI
                     DFDVHHGNGTEEILAGDERALMASFFQHPFYPYTGTENVASNMVNVPLPAGTRGDVVR
                     TVVDETWLPRLEQFRPEMIFVSAGFDAHREDDLGQMGLVEADYVYITRRVMEIARRHA
                     GGRIVSSLEGGYNLSVLGRSVAAHVKTLAQL"
     gene            2631..4277
                     /locus_tag="AC16HB_15165"
     CDS             2631..4277
                     /locus_tag="AC16HB_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR5608177.1"
                     /translation="MPSTIFDHGLDRNAGNYAPLSPLTFLERSASVFPDRTAVIHGGS
                     IRRTWSELYARCRRLADALAARGLGRGDTVAVMLPNAPEMVEAHFGIPMAGCVLNAIN
                     TRLDASSVAFMLEHGEARLFIVDREFAPVAKAALTQVGRKIEVIDVLDPVCEGPGDRL
                     GTLDYETFIAAGAPDFAWQPPADEWDAIALNYTSGTTGNPKGVVYHHRGAYLNAVSNI
                     LVWAMPQHPVYLWTLPMFHCNGWCFPWTVAAMSGTHVCLRKVEAKAVFDTIRDHGVTH
                     YCGAPIVHALLVNAPPELKQGIAHRVYAMVAGAAPPAALIEGMERMGFSLTHVYGLTE
                     VYGPATVCPQQAGWQTLDVGARAMLNARQGVRYPMEEAVDVLDPQTMQPVPRDGETIG
                     EVMFRGNITMRGYLKNEKATREAFAGGWFHSGDLAVVHPDGYLKIKDRSKDIIISGGE
                     NISSIEVEDVLYRHPAVMAAAVVAQPDPKWGEVPCAFVELKQGARASAEELIAHCRQH
                     LAGFKIPKRVVFGEIPKTSTGKIQKFQLRQQAKSASAIDI"
     gene            4287..5081
                     /locus_tag="AC16HB_15170"
     CDS             4287..5081
                     /locus_tag="AC16HB_15170"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR5608178.1"
                     /translation="MSAVPQALEPVLLRDDADGIATLTLNRGSQFNLLTGEMIDALQA
                     AFDAIARDESVRVIVLAAAGKGFCAGHDLKEIRALREQPKIEQLFVRCNRMMVTMTRL
                     PQPVIARVQGAAAAGGCQLVAQCDLAVAVDTAKFTTPGVSWGFFCSTPGVALARNLPR
                     KRALEMLLTGDMIDAQTALQWGLVNRVVPADRLDAEVRGLATKIAAKPRATIADGKRA
                     FYQQVDSGLERAYELAGCAIASSFAHEDGREGMDAFIDKRAPRWAR"
     gene            complement(5095..5673)
                     /locus_tag="AC16HB_15175"
     CDS             complement(5095..5673)
                     /locus_tag="AC16HB_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608179.1"
                     /translation="MEKPRLFMKIVSVSDAARASNDAPRTTLRIENGVPAASTTTPAE
                     ATDLPTDRRVILERELDRLQQAIQIDQQAAGQHLNWLLLSQALFMNAFLIVLVLGGAV
                     PLTFAVWLLVGLAALGVIAAVVLHATLRRTREEIAMLSLQRRAVEMSLQKEFGRVPLF
                     PPGRNEAPSAALPTIFIAAWALLVAYAVLMPR"
     gene            complement(5781..>6431)
                     /locus_tag="AC16HB_15180"
     CDS             complement(5781..>6431)
                     /locus_tag="AC16HB_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5608180.1"
                     /translation="TCAVLIEPIQGEAGIVVPPDGFLRAARRLCDERKVLLILDEIQS
                     GLGRTGRWFAFQHEGIRPDGLILGKALGGGLLPVSCFVATEELMNVFDPGSHGSTFGG
                     NPLAAAVALEALKVLEDEGLVTRSAVLGTHLLQRLRAIGSPLIRAVRGRGLWVGVDLD
                     PARVSARKVVERMAERGVLSKDTHESVIRFAPPLTIARASLDYGIDVFAEVLREFE"
BASE COUNT         1088 a         2138 c         2217 g          988 t
ORIGIN      
        1 ggatcgcgcg gccagccgcc acgcgatcat cttctgcgga tcgccgggct ggtaagggcg
       61 cacgcctgcg aagtcgtcgc ccgtcgacgc caggcccacg ccttcgcctt cgccgcccgc
      121 ggcgaccggc agcggcggtg cgtcgtcctc cgggttcggg tacacgatcg cggtgagcgg
      181 cggcgtgtag tacgcccatg cctgccacag cccgaacgga aagcgcgtct cgatgcgtat
      241 gcgcggacac gcaagcctgc cgcgccgcgc cgcgggcagt ccaagccgca ccgtgcgcgc
      301 gctgccggcg gggatgtcga cgtggaccga agccacccgc gcgcgcgccg gcgacagcag
      361 tgcgaatccg agcgcgtgac gcggctctgc ggtcgggttg gtggcgacga tctcgaacgt
      421 cgccacgtcg ccggcaaaca cgttttccac gccgtggccg cgcagttgca cgcgtgtgag
      481 gttgcggtgc gtgtgataca tggccaccag cgcgatgctg gtcaccagga aagtcagcat
      541 gaagcccagt tgcagcgcat agttgatcga gccgatcagc agcaccagca tcgccacgcc
      601 gaacaccagg ccggcgcgcg tcggaacgat gtagacgcgc cgccggtcga gcagcacgtc
      661 gccgtgctcg atcccatcgc gtccggcgat ccaggccgcg atgcggatcg aaagcggatg
      721 tgtggtgaag ctcacgggac cgcgacggcc tgcgccagtt gcgcgaccag ttcgcgcccg
      781 tgcagaacgc ggccgccttc gcgctgggtg acgtgcagcc ggtggcccgc aaccgcgggc
      841 agcacgttct gcacgtcttc gggaatgacg tgctcgcggc cgtcgaacca ggcccacgcg
      901 cgcgcggccg cgagcagcgc cagcccggcg cgcgggctca tgccctcggc atacttgccc
      961 gatgcgcgcg tgtgcgcgag cagcgcctgc acgtagtcga gcagggcgtt ggagacgaat
     1021 accttcttcg cctcggcctg cgcggcgagc agatcggccg gccgcgcgac ggccgacaac
     1081 gtgcgcagca tgtcgcgccg cgactggccg gcgagcaggg cgcgctcggc ggcgcggtcg
     1141 ggatagccga ggctgatgca catcaggaag cgatcgagct gcgactcggg cagcggaaag
     1201 gtgccgacct ggtgcagcgg gttctgcgtg gcgatcacga agaacggctg tggcagcggc
     1261 cgcgacagcc cgtcgaccga aacctggctt tcctccatcg cctccagcaa cgcgctctgc
     1321 gtcttcgggc tggcgcggtt gatttcgtcg gccagcagca cctcggtgaa gatcgggccc
     1381 ggatggaaaa cgaactgcgc gttggtgcgg tcgtataccg aaacgccgat gacatccgaa
     1441 ggcagcaggt cgctggtgaa ctggatgcgc cggaacggca ggccgagcga tagcgcaatc
     1501 gcgtgggcca gcgtggtctt gccgaccccc ggcgcgtcct cgatcagcag atggccgccg
     1561 gcgagcaggc aggtcaggct ctgccgcacc tgcaggtcct tgccgacgat cacgcccgag
     1621 atctgggccg cgcatcttcg aagttgatcc agcattaaac tcggcccgag ggcagcagag
     1681 gagacagaag gtcgtgcata ccggattctt cacgcaccac gtgtgcgcgg cgcacgagat
     1741 gggatcgtgg cacccggaga gtccggagcg cctggcggcg atccaggacc atctgatcgc
     1801 gaccggcctg gcgccgcaca tcgaatacgt ggacgccccg cccgcgaccc tcgatcaggt
     1861 cgcgcgcgcg catgatcgcg agtacatcga tgcattgcga tcgaggatac caaagcacgg
     1921 ctatcacccg ctcgatccgg acacgtcgat gaatccgtcg acgtgggagg cggcgctgcg
     1981 ttccgccggg gccgtcatcg cggcaaccga ccaggtggtc ggtggcggcc tcgacaacgc
     2041 attctgcgcg gtgcgtccgc ccgggcacca tgcgcggccg ggagcggcaa tgggtttctg
     2101 cttcttcaac aacgtcgccg tcggcgcgct gcacgcgctg cagcaccatg gactgaagcg
     2161 cgtggcggtc atcgacttcg acgtgcacca cggcaacggc acggaagaga tcctcgccgg
     2221 cgacgagcgc gcgctgatgg cgagcttctt ccagcacccg ttctatccgt acacgggcac
     2281 cgagaacgtc gcttcgaaca tggtcaacgt gccgcttccg gcaggcaccc ggggcgacgt
     2341 ggtgcgtacc gtggtcgatg aaacctggct gccgcggctg gagcagttcc ggccggaaat
     2401 gatcttcgtc tcggccgggt tcgacgcgca ccgcgaagac gaccttgggc agatgggatt
     2461 ggtcgaggcc gattacgtgt acatcacgcg ccgcgtgatg gaaatcgcgc gccgccatgc
     2521 cggcggtcgc atcgtcagtt cgctcgaagg cggctacaac ctgagcgtgc tcggccgcag
     2581 cgttgccgca cacgtgaaga cgctggcgca gctttagaaa gaggtgatcc atgccgtcga
     2641 cgatcttcga tcacggactg gacaggaacg cgggcaacta tgcgccgttg agcccgctga
     2701 cgtttctcga gcgctcggcg tccgtcttcc cggatcggac cgcggtcatc cacggcggat
     2761 caatccgccg cacctggtcc gagctgtacg cgcgctgccg ccggctggcc gatgcactcg
     2821 ccgcgcgcgg gctgggtcgc ggcgacacgg tcgcggtgat gctgcccaac gcgccggaga
     2881 tggtcgaggc gcacttcggc attccgatgg cgggctgcgt gctgaacgcg atcaacacgc
     2941 ggctcgacgc gtcgtcggtc gcatttatgc tggagcacgg cgaggcgagg ctcttcatcg
     3001 tcgaccgcga gttcgcgccg gtcgccaagg cggcgctgac gcaggtcgga cgcaagatcg
     3061 aggtgatcga cgtgctcgat cccgtctgcg aagggccggg cgatcggctg ggcacgctcg
     3121 actacgagac gttcatcgcc gccggcgctc ccgatttcgc gtggcagccg ccggcggatg
     3181 agtgggatgc gatcgcgctg aactacacgt ccgggacgac gggcaatccg aagggcgtcg
     3241 tctaccacca ccggggcgcg tacctgaacg cggtgtcgaa catcctcgtg tgggcgatgc
     3301 cgcagcatcc ggtgtacctg tggacgttgc cgatgttcca ctgcaacggc tggtgtttcc
     3361 cgtggacggt ggccgcgatg tcgggcacgc acgtgtgcct gcgcaaggtc gaggccaagg
     3421 cggtgttcga cacgattcgc gaccacggcg tcacgcacta ctgcggtgct ccgatcgtgc
     3481 acgcattgct cgtcaatgcg ccgccggagc tgaagcaggg catcgcgcac cgcgtatacg
     3541 caatggtcgc cggcgcggct ccgccggcgg cgctgatcga gggcatggaa cggatgggct
     3601 tcagcctcac ccatgtctat ggcctgaccg aggtgtacgg tccggcgacc gtgtgtccgc
     3661 aacaggcagg atggcagaca ctcgacgtcg gcgcgcgcgc gatgctcaat gcgcgccagg
     3721 gcgtccgtta cccgatggag gaggccgtcg acgtgctcga cccgcagacg atgcagccgg
     3781 tcccgcgcga cggcgagacg atcggcgaag tgatgttccg cggcaacatc acgatgcgcg
     3841 gctatctgaa gaacgagaag gccacgcggg aggcgttcgc cggcggctgg ttccactccg
     3901 gcgacctggc ggtcgtccat cccgacgggt atctgaagat caaggaccgc agcaaggaca
     3961 tcatcatctc cggcggcgag aacatctcgt cgatcgaagt ggaggatgtg ctgtaccgcc
     4021 atcccgcggt gatggccgcc gcggtcgtcg cgcagccgga tccgaagtgg ggcgaggtgc
     4081 cgtgcgcgtt cgtcgagctg aagcaggggg cccgtgcgag cgcggaggaa ctgattgcgc
     4141 attgccggca gcacctggcc ggcttcaaga ttcccaagcg ggtcgtgttc ggcgagatcc
     4201 cgaagacatc gacgggcaag atccagaaat tccagctgcg gcagcaggcc aagtctgcgt
     4261 ccgcgatcga catctaggag acgaccatga gtgccgtgcc tcaagccctc gaacccgtac
     4321 tgctgcgcga cgatgccgac ggcatcgcga cgctgaccct gaaccgcgga tcgcagttca
     4381 acctgctgac cggcgagatg atcgatgcgc tgcaggcggc tttcgatgcg atcgccaggg
     4441 acgaatcagt gcgcgtgatc gtgctggcag cggccggcaa gggcttctgc gccggccacg
     4501 acctgaagga gatccgcgcg ctgcgcgagc agccgaagat cgagcagctc ttcgtccgct
     4561 gcaaccgcat gatggtgacg atgacgcgcc tgccgcagcc ggtgatcgcg cgcgtgcagg
     4621 gcgctgccgc cgccggtggc tgccagctcg tggcccagtg cgatctggcc gtcgctgtcg
     4681 acacggcgaa gttcacgacg cccggcgtgt cgtggggctt cttctgctcc acgccaggcg
     4741 tcgcgctggc gcgcaacctg ccgcgcaagc gcgcgctgga gatgctgctg accggcgaca
     4801 tgatcgatgc acagaccgcg ctgcaatggg ggctggtaaa ccgcgtcgtg ccggcggacc
     4861 ggctcgacgc cgaagtccgg ggccttgcca cgaagatcgc cgccaagcca cgcgccacga
     4921 tcgccgacgg caagcgcgcg ttctatcagc aggtcgattc cggtctagag cgcgcgtacg
     4981 aactggcagg ctgtgcgatc gccagcagct tcgcgcacga ggatgggcgc gaaggcatgg
     5041 acgcgttcat cgacaagcgc gcgccgcgct gggcgcgctg acctgccgcg cgattcagcg
     5101 cggcatcagc accgcgtagg cgaccagcag cgcccacgcg gcgatgaaga tcgtcggcag
     5161 cgccgccgag ggagcctcgt tgcggcccgg cgggaacagc ggcacgcggc cgaattcctt
     5221 ctgcagcgac atttccaccg cgcgccgctg caggctgagc atcgcgatct cctcgcgcgt
     5281 gcgtcgcagc gtggcgtgca gaacgaccgc ggcgatcacg ccgagcgcgg cgagtcccac
     5341 cagcagccag acagcgaacg tcagcggcac cgcaccgccg agaacaagca cgatcagaaa
     5401 tgcgttcatg aacagcgctt gtgacagcag cagccagttc aggtgctgcc cggcggcctg
     5461 ctgatcgatc tggatcgcct gttgcagccg gtcgagctcg cgttcgagaa tcacgcggcg
     5521 atcggttggc aggtccgtcg cttccgccgg cgtcgtcgtg gacgcggccg gcacgccgtt
     5581 ttcgatgcgc aatgtcgtgc gcggcgcatc gttcgacgcg cgcgctgcat ccgacaccga
     5641 gacaatcttc atgaacagcc ttggcttttc catcgattcc aaggccgaaa tatgacgaac
     5701 gttcgatgtg gtgttgatcc tgccttcgga tgagaaaacg tcggatgaac gggtggtcag
     5761 cggtaatgcg tagcccgcca ctactcgaat tcgcgcagca cctcggcgaa cacatcgata
     5821 ccgtagtcga gtgatgcacg tgcgatcgtc agcggcggcg cgaagcgaat caccgattcg
     5881 tgcgtgtcct tcgacagcac accgcgctcg gccatgcgct cgaccacctt gcgtgccgac
     5941 acgcgcgccg gatcgaggtc gacgccgacc cacaggccgc ggccgcgcac ggcgcggatc
     6001 agcggcgacc cgatcgcccg cagccgctgc agcagatgcg tgccgagcac cgcgctgcgc
     6061 gtcaccagcc cctcgtcttc cagtactttc agcgcttcga gcgcgacggc ggcggcgagc
     6121 ggattgccgc cgaacgtcga gccgtggctg ccggggtcga agacgttcat cagttcctcg
     6181 gttgcgacga aacagctgac cggcaacagc ccgccgccga gcgccttgcc gagaatcagc
     6241 ccgtcgggcc ggatgccttc atgctggaac gcgaaccaac ggccggtgcg gccgaggccc
     6301 gactggattt cgtcgaggat cagcaggacc ttgcgctcgt cgcacagtcg tcgtgccgcg
     6361 cgcaggaatc cgtccggtgg caccacgatg ccggcctcgc cctggatcgg ctcgatcagc
     6421 accgcgcacg t
//
LOCUS       DBNHBY010000633         3225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_855531, whole genome shotgun sequence.
ACCESSION   DBNHBY010000633 DBNHBY010000000
VERSION     DBNHBY010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_855531"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC16HB_15185"
     CDS             complement(<1..517)
                     /locus_tag="AC16HB_15185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608181.1"
                     /translation="MSDASLARKQLALLVTCQALLYVNNVTLISVNALTGLALSPNPL
                     LATVPITTYIIGSSLTTLPASFAMARWGRRAGFMFGSLMGLVGTLLAATAMFVHSFVL
                     LCAGTFVIGLYNAFAQYLRFAAVDVADAYDKTLKERAISWVLAGGLAGGVIGPEMSKL
                     TREALPITYAGT"
     gene            619..1539
                     /locus_tag="AC16HB_15190"
     CDS             619..1539
                     /locus_tag="AC16HB_15190"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR5608182.1"
                     /translation="MSMADRDGWIWYDGKLVPWRDATTHVLTHSLHYGLAVFEGVRAY
                     KTVDGTAIFRLKEHTERLFNSAKIFMMEIPHTQEQLNEAQREVVRANKLDSCYLRPLV
                     FYGSEKMGVSPKGARVHVAIAAWPWGAYLGEEGLQKGIRVKTSSYQRHHINVSMVRSK
                     TAGHYVNSVLANLEATNHGYDEALLLDTQGFVAEGAGENLFIVRDGEIFEPQMVSGLI
                     GVTRRSVIELAREIGKNVTQMPMTRDDVYLADEAFFTGIAAEVTPIRELDGRAIGKGS
                     RGPVTEKIQNLFFDVVNGRNAKFKHWLTKV"
     gene            1536..1733
                     /locus_tag="AC16HB_15195"
     CDS             1536..1733
                     /locus_tag="AC16HB_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR5608183.1"
                     /translation="MTTTELPVVELAAKDLPAFCPNPQMPVWNHHPRVFLDVATTGEA
                     MCPYCGTKYRLKAGEAAKGHH"
     gene            1750..2193
                     /locus_tag="AC16HB_15200"
     CDS             1750..2193
                     /locus_tag="AC16HB_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5608184.1"
                     /translation="MSRLTKPLLFASSAECEQAFYEALEAGDIDALTDLLHDDDDVYC
                     VHPGGPRLIGFAAVRASWAAILANGAVRIRAQARKTLETPTVALHNVIEEVVVEQGRS
                     PKIVHVIATNGYVKTPSGWKMVLHHGSPAPAGPVEEFEVPHGPLH"
     gene            2328..>3225
                     /locus_tag="AC16HB_15205"
     CDS             2328..>3225
                     /locus_tag="AC16HB_15205"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR5608185.1"
                     /translation="MSNASSNYSAPWWLPGGHLQTIVPAKLLPAPAVEYRRERWDTPD
                     DDFIDVDFALPEPVAAGAPVLVLFHGLEGSSQSHYARVIMRTFADRGWRGLVPHFRGC
                     SGEPNRLPRAYHSGDSDEGDWILRRVHARWPDAPLYAAGISLGGNMLAKWLGERAEDA
                     QFVTAAASIGSPLDLAAGGAALARGFNLVYTKMFLATLRTKALAKIEAFPGIARAELI
                     RAARDLYDFDNEYTAPVHGFRDTDDYWARASGKPWLGGVRVPHLGLNARNDPFVPAPS
                     LPSTHEVSRAVRLEQPEHGGHIG"
BASE COUNT          561 a         1081 c         1086 g          497 t
ORIGIN      
        1 aagtgcccgc ataggtgatc ggcagtgcct cgcgcgtgag cttgctcatc tccgggccga
       61 tcacgcctcc cgccaggccg cccgccagca cccacgagat cgcgcgttcc ttgagcgtct
      121 tgtcgtacgc gtcggcgaca tcgacggcgg cgaagcgcag gtattgcgca aacgcgttgt
      181 acagaccgat cacgaaggtg cccgcgcaca gaagcacgaa gctgtgaacg aacatcgcgg
      241 tggccgccag cagcgtgccc acgaggccca tcagcgagcc gaacatgaac ccggcgcgcc
      301 gaccccagcg cgccatcgcg aacgacgccg gcagcgtggt cagcgacgag ccgatgatgt
      361 aggtcgtgat cggtaccgtc gccagcagcg ggttcggcga gagcgcgagg ccggtcagcg
      421 cgttgaccga gatcagtgtg acgttgttga cgtacagcag cgcctggcag gtgaccagca
      481 gcgcaagttg cttcctcgcc agcgatgcat cggacatcgg cttcctctcg gtacccaata
      541 gtatcgggcc gcgtttcgcg ccatcgctac aattcgcgct cgttcgattg caaccgcaac
      601 acaaccggag actgccccat gtcgatggcc gaccgcgatg gatggatctg gtacgacggc
      661 aagctggtgc catggcgcga cgcgaccacg cacgtgctca cgcattcgct gcactacggg
      721 ctcgccgtgt tcgagggtgt gcgcgcctac aagacggtcg acggcaccgc gatcttccgc
      781 ctgaaggagc acaccgagcg gcttttcaac tcggccaaga tcttcatgat ggagatcccg
      841 cacacgcagg agcaactcaa cgaggcgcag cgcgaggtgg tgcgcgccaa caagctcgat
      901 tcctgctatc tgcggccgct ggtgttctac ggcagcgaga agatgggggt gagtcccaag
      961 ggtgcgcgcg tgcacgtggc gatcgcggcc tggccgtggg gtgcgtacct cggcgaggag
     1021 gggctgcaga agggcatccg cgtgaagacg tcctcgtacc agcgccacca catcaacgtg
     1081 tcgatggtgc gctcgaagac cgccggccac tacgtgaact cggtgctggc gaacctggag
     1141 gcgaccaacc acggctacga cgaggcgttg ctgctcgaca cgcagggctt cgtcgccgag
     1201 ggcgcgggcg aaaacctgtt catcgtccgc gacggcgaga tcttcgagcc gcagatggtg
     1261 tcggggctga tcggcgtcac gcgccgctcc gtgatcgaac tggcgcgcga gatcggcaag
     1321 aacgtcacgc agatgccgat gacgcgcgac gacgtgtacc tggccgacga agcgttcttc
     1381 accggcatcg cggccgaagt cacgcccatc cgcgaactcg atggtcgcgc gatcggcaag
     1441 ggctcgcgcg ggccggtcac cgagaagatc cagaatctct tcttcgacgt ggtcaacggc
     1501 cgcaacgcca agttcaagca ctggttgacc aaggtatgac gactactgaa ctccccgtgg
     1561 tcgaactcgc ggccaaggac ctgcccgcgt tctgccccaa tccgcagatg ccggtgtgga
     1621 accaccaccc gcgcgtattc ctcgatgtcg ccaccaccgg cgaagcgatg tgcccgtact
     1681 gcggcacgaa gtaccgcctg aaggcgggcg aggccgccaa gggacatcac tgaacggcgc
     1741 cacgccacga tgtcgcgcct gaccaagccg ctgctgttcg cctcgagcgc ggagtgcgag
     1801 caggcgttct acgaggcgct ggaggccggc gacatcgacg cgctcaccga cctgctgcac
     1861 gacgacgacg atgtctactg cgtccacccg ggcgggccgc ggctgatcgg ctttgccgcg
     1921 gtgcgcgcgt cgtgggcggc gattctcgcg aacggcgcgg tgcgcatccg ggcgcaggcg
     1981 cgcaaaacgc tcgagacacc gacggtggcg ttgcataacg tgatcgagga agtcgtggtc
     2041 gagcaggggc gcagcccgaa gatcgttcac gtgatcgcta ccaacggcta cgtgaagacc
     2101 ccatcgggct ggaagatggt gctgcatcac ggatcgcccg cgcccgcggg accggtcgag
     2161 gaattcgagg tgccgcacgg gccgctgcac tagcagcgat gcctcggatg tacgcgacgg
     2221 ctgcgccctc gctcgtgcac cctcggcggc aatgtcagac acgctgcctt gaggcggctc
     2281 ggcggcggcg cgcgcagcaa cagcaacgca agacctgaaa ccccgcggtg tccaacgctt
     2341 cgtcgaacta ctccgcgccg tggtggctgc cgggcgggca tctgcagacc atcgtccccg
     2401 ccaagcttct gccggcaccc gcggtcgagt accggcggga gcgctgggac acgcccgacg
     2461 acgatttcat cgatgttgac tttgcgctgc ccgagcccgt cgccgcgggc gcgccggtgc
     2521 tggtgctgtt ccacgggctc gagggcagct cgcagagcca ctacgcgcgc gtgatcatgc
     2581 gtactttcgc cgatcgcggc tggcgcggac tggtgccgca ctttcgcggc tgctcgggcg
     2641 agccgaatcg gttgccgcgc gcctaccact cgggcgattc cgacgagggc gactggatac
     2701 taagacgcgt gcacgcgcgc tggccggacg cgccgctgta cgcagccggc atctcgctcg
     2761 gcggcaacat gctcgcgaag tggctgggcg agcgcgccga ggacgcgcag ttcgtcaccg
     2821 cagccgcgtc catcggcagc ccgctcgatc tcgcggccgg cggcgcggcg ctggcgcgcg
     2881 ggttcaacct cgtctacacg aagatgttcc tcgccacgct gcggaccaag gcgctggcga
     2941 agatcgaggc gtttcccggc atcgcgcgcg ccgaactgat tcgcgccgcg cgcgatctgt
     3001 acgacttcga caacgagtac accgcaccgg tgcacggctt tcgcgatacc gacgactact
     3061 gggcgcgtgc gtcgggcaag ccgtggctcg gcggcgtccg cgttccgcac ctggggctga
     3121 atgcgcgcaa cgatccattc gttcccgcgc cgagcttgcc cagcacgcac gaagtgtcgc
     3181 gcgcggtgcg gctcgagcag ccggagcatg gcgggcacat cggtt
//
LOCUS       DBNHBY010000634         1948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_653206, whole genome shotgun sequence.
ACCESSION   DBNHBY010000634 DBNHBY010000000
VERSION     DBNHBY010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_653206"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="AC16HB_15210"
     CDS             <1..1722
                     /locus_tag="AC16HB_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608186.1"
                     /translation="LPLPREPMTSDPRPWLRFYGAVPHTLSYPPVTLYVAVAATAQKT
                     PDAVAWDFFGTTSTYRELLAAIDQCAGALAALGMRAGDRLLISMPTAPAGIIAFYAAN
                     KLGVVPALIHPLSTAPEIEHYLNASGARVALTLDAFYGVLAQTKPKLPLAHILLARIG
                     DYLAPLKRFGFWLTKGRKIAAIPNDARVRWWKELMAAPPPRAMRANTGPDDAAAILFS
                     GGTTGVPKGILLSNRNLIAEGMQAAAWGGMAPGDSILAILPIFHGFGLGVCVNAAFMA
                     GGKSILVPQFSADIVAKLLRTARPNLLVGVPTLYDALSQDPSLAKADLSCLRACFSGA
                     DTLPRPVKERFEQLVASRGGKVRLLEGYGLTEAVTAIMAMPVSEYREASIGIPFPDMD
                     AKIVRLGTTEELPAGEEGEICVAGPAVMLGYLDDPEATAQTLKVHADGRTWLHTGDLG
                     RRDADGFFYFTVRAKRMIKSSGFNVYPAQVESVLYQHPLVAEACVIGVPDASQVERVK
                     AVVVLKDPAQESDATAQQLIDFCRERLIKWSCPREIEFRRVLPKTRVGKIDFKVLVAE
                     HIAKGGA"
     gene            1719..>1948
                     /locus_tag="AC16HB_15215"
     CDS             1719..>1948
                     /locus_tag="AC16HB_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5608187.1"
                     /translation="MNDGDRVAAVLRAHGVPYLVTLCGGHSSPILIAARERGIRVVDT
                     RDEATAVFAADAVARLTGAPGVVAVTAGPGLT"
BASE COUNT          297 a          678 c          661 g          312 t
ORIGIN      
        1 cttcccctgc cgcgcgaacc catgacctcc gacccacgcc cctggctgcg cttttacggc
       61 gcagttcccc acaccctcag ttacccgccg gtcacgctgt acgtggcggt cgccgccacc
      121 gcgcagaaga cgccggacgc cgtcgcgtgg gacttcttcg gcaccacgtc cacctatcgc
      181 gaactgctcg cggcgatcga tcaatgcgcg ggcgcgctcg ccgcgctcgg catgagggcc
      241 ggcgaccgcc tgttgatctc gatgccgacc gcgccggcgg gcatcatcgc gttctacgcg
      301 gcgaacaagc tcggcgtcgt gccggcgctg atccatccgc tgtcgaccgc tccggagatc
      361 gagcactatc tgaacgcaag cggcgcgcgg gtcgcgctga ccctcgacgc gttctacggc
      421 gtgctcgcac aaaccaagcc gaaactgccg ctcgcgcaca tcttgctggc gcgcatcggg
      481 gactatctgg cgccgctgaa gcgcttcggc ttctggctca ccaagggccg caagatcgcg
      541 gcgatcccga acgacgcgcg cgtgcggtgg tggaaggaac tgatggctgc accgccaccg
      601 cgcgcaatgc gcgcgaacac cggccccgac gatgcggccg cgatcctttt ttccggcggc
      661 acgaccggcg tgccgaaggg catcctgctg tcgaatcgca acctgatcgc cgagggcatg
      721 caggccgcgg cgtggggcgg catggcccct ggcgattcga tcctcgcgat cctgccgatc
      781 ttccacggct tcggtctcgg cgtgtgcgtg aacgccgcgt tcatggccgg cggcaaatcg
      841 atcctggtgc cgcagttctc ggccgatatc gtcgcgaagc tcctgcgtac cgcgcggccg
      901 aacctgctcg tcggggtgcc gacgctgtac gacgcgctgt cgcaggaccc gtcgctcgcc
      961 aaggcggatc tatcgtgcct gcgcgcatgc ttctccggcg ccgacacgct gccgcgtccg
     1021 gtcaaggagc gcttcgagca actcgtcgca tcgcgtggcg gcaaggtgcg gctgctggaa
     1081 ggctatggcc tgaccgaggc ggtgacggcc atcatggcga tgccggtgag cgagtatcgc
     1141 gaagcgtcga tcggcattcc gtttcccgac atggatgcca agatcgtccg cttgggcacg
     1201 accgaggagc tgcccgcggg cgaagagggc gagatctgcg tggccggccc cgccgtgatg
     1261 ctgggctacc tcgacgatcc cgaggcgacc gcgcagacgc tgaaagtgca cgccgatggc
     1321 cgcacctggc tgcacaccgg cgacctcggc aggcgcgacg ccgacggctt cttctacttc
     1381 accgtgcgcg ccaagcgcat gatcaagtcg tccggcttca acgtatatcc ggcgcaggtg
     1441 gaaagcgtcc tgtaccagca tccgctggtg gccgaggcgt gcgtgatcgg tgtgcccgac
     1501 gcgtcacagg tcgagcgcgt gaaggcggtc gtcgtgctga aggaccccgc gcaggaaagc
     1561 gatgcgaccg cgcagcagct gatcgacttt tgccgcgagc ggctgatcaa gtggtcgtgc
     1621 ccgcgcgaga tcgagttccg gcgcgtactg ccgaagacgc gcgtcggcaa gatcgacttc
     1681 aaggtgctgg tcgccgagca catcgcaaag ggcggcgcat gaacgacggt gatcgcgtcg
     1741 ccgccgtgct gcgcgcacac ggcgtaccgt acctggtcac gctgtgtggc gggcacagct
     1801 cgccgatcct catcgccgcc agggagcgcg gcatccgcgt cgtggacacg cgcgacgagg
     1861 cgaccgcggt gtttgccgcc gacgcggttg cgcggctgac cggcgctccg ggcgtcgtcg
     1921 ccgtgacggc cggtccgggg ctcaccaa
//
LOCUS       DBNHBY010000635        17571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_992321, whole genome shotgun sequence.
ACCESSION   DBNHBY010000635 DBNHBY010000000
VERSION     DBNHBY010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_992321"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="AC16HB_15220"
     CDS             complement(<1..419)
                     /locus_tag="AC16HB_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein"
                     /protein_id="HIR5608188.1"
                     /translation="MLAILTAAGCATQQRSASVYRAGEAQREQIVRMATVESVREVTL
                     DRGQTGVGTGAGAIVGGVAGSSVGGGKGSVIGAVAGAVAGGIVGQAVENQSSKVPAIE
                     ITVRLDSGDLRAIVQEIDGQQFKPGDRVRLLSEGGGRG"
     gene            complement(579..1514)
                     /locus_tag="AC16HB_15225"
     CDS             complement(579..1514)
                     /locus_tag="AC16HB_15225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5608189.1"
                     /translation="MSTLICGSIAYDSIMVFEGRFKDHILPEQVHILNVAFLVPQLRR
                     EFGGCAGNIAYSLRMLGGTPLPMATVGDDAQPYLDRLDALGIDRRHVRHVPGTYTAQA
                     FITTDLDDNQITAFHPGAMSHSHLNAVPGAGVVRLGIIAPDGRDGMLQHAQQFADHGV
                     PFVFDPGQGLPMFDGDELKRLIARAHYVAVNDYEARLLIERTGMDLGTLARKVKALVV
                     TLGGKGSEIYADGQVLEIPAAPPRAVSDPTGCGDAYRAGLLYGIENGMDWQTTGRLAA
                     VMGTLKIEHSGAQNHSADRALIAKRFVETFGYRPW"
     gene            complement(1534..2580)
                     /locus_tag="AC16HB_15230"
     CDS             complement(1534..2580)
                     /locus_tag="AC16HB_15230"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HIR5608190.1"
                     /translation="MALATRCPSCHALFRVVADQLKLRGGLVRCGGCGHVFDAIGTLS
                     YLDDASVRPAATPAPATIEPTPAPPPVVPPAEFAGQPATVPVEGPSVPDRRDETIAAA
                     TVPDESNAEFAIDRRQDESHVESRPIVPAADEMHDAEAIDAIDSEEQPADRSAEVDGA
                     ASELVDEAVVPDTSAPAFLHEGSTPRRRVVTLVYALGAALAALALALQLVLMYRTEIA
                     AQLPATRGVLVAVCQVMHCAVGWPMHGELLAIVGSELQSLPGTNALELTAVVRNRAEI
                     TLALPALEVTLTDTQNRAVARRIFGPIDYLGPSEAAARLADGLQAGADMIVRITFEAR
                     GLNVAGFVVYPFYL"
     gene            complement(2586..3488)
                     /gene="prmA"
                     /locus_tag="AC16HB_15235"
     CDS             complement(2586..3488)
                     /gene="prmA"
                     /locus_tag="AC16HB_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR5608191.1"
                     /translation="MQREVVVIVDAEDVDTLSDALLDAGALSVAVEDADADLEGEQPL
                     FGEPGLEPARPAWRRSRLIVMVDAAATAAEMLASAAASIDRPPPAIIATREVGDADWV
                     RLTQAQFAPTRLSDRLWIVPTWHEPPDADAINIRLDPGVAFGTGTHPTTRLCLSWLAQ
                     ARLANARVLDYGCGSGILAITAAKLGAREVVGTDVDPQALQAARTNSAINQVTARYTA
                     PQELGRGLFDVILANILANPLILLAPALVARLADGGQIVLSGILERQAPQVIDAYARV
                     CSELALRVWRVDDGWCCIVTQRTG"
     gene            complement(3508..4866)
                     /gene="accC"
                     /locus_tag="AC16HB_15240"
     CDS             complement(3508..4866)
                     /gene="accC"
                     /locus_tag="AC16HB_15240"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5608192.1"
                     /translation="MFGKVLIANRGEIALRVQRTCRELGIKTVVVHSTADTDAKYVKL
                     ADESVCIGPPPSRDSYLNIPAIISAAEVTDAEAVHPGYGFLSENADFAERVEKSGFAF
                     IGPRPESIRLMGDKVSAKKAMIAANVPTVPGSDGTLPDEPREIVKIARAIGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVSMTRQEAQSAFGNPAVYMERFLENPRHIEIQVLADQ
                     HRNAVWLGERDCSMQRRHQKIIEEAPAPGIARKLVERIGDRCAEACRKIGYRGAGTFE
                     FLYENGEFCFIEMNTRVQVEHPVTEMVTGIDIVQQQIRIAAGERLPFRQRDIALRGAA
                     IECRINAEDPYKFTPSPGRITAWHPPGGPGVRVDSHAYSGYFVPPHYDSLIGKLICYG
                     DTRSQALARMRVALSEMIVEGISTNIPLHRELMLDEKFVLGGTSIHYLEKKLAARQLN
                     QP"
     gene            complement(4974..5453)
                     /gene="accB"
                     /locus_tag="AC16HB_15245"
     CDS             complement(4974..5453)
                     /gene="accB"
                     /locus_tag="AC16HB_15245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR5608193.1"
                     /translation="MDLRKLKTLIDLVAESGISEIEVTEGEDKVRIVKHGPPPPMPVA
                     ALPAVTAAQAAMAAQPAAAATPTTPPVAPSMPGAEPLKGQIVKSPMVGTFYRASSPGA
                     NPFVDVGQAVKPGDTLCIIEAMKLLNEIEAEVAGVVKECLVENGQPVEYGQPLFVIE"
     gene            complement(5611..6144)
                     /locus_tag="AC16HB_15250"
     CDS             complement(5611..6144)
                     /locus_tag="AC16HB_15250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5608194.1"
                     /translation="MRRRWLLAATASSALAMGIGVALWREAHRSGSAAGAAALFAATY
                     TDADGIARPLAQWRGTLLVVNFWATWCAPCVEEMPALDRVRDEFGAHGVEVIGIGIDS
                     ADRIRAFRDKLGIGLPLLVAGSDGSELARTLGNLAGVLPYTVLVARSGAIEQRHVGQI
                     RPEQLRAWLNAQLAAGG"
     gene            complement(6146..6796)
                     /locus_tag="AC16HB_15255"
     CDS             complement(6146..6796)
                     /locus_tag="AC16HB_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608195.1"
                     /translation="MSHPERDDLRTELAAAAARLIAEDGLDYATAKQKAAQAILGNAV
                     AARGALPDNAQIEMELRRHLSTFLPRHAAVLAALRQLALQLMQRLDRFRPHIVGAVLN
                     GTATEHSDIHLQVFVDSAKDVEMFLLEQGVEYDVIEGGRQPGEPLEVLHLVVRPARTR
                     GMPPRVGVVIDVLDTDALRVAPKYRSTAPGLHPVEAAGRANATMLGALIAARDPEA"
     gene            6914..8269
                     /gene="mpl"
                     /locus_tag="AC16HB_15260"
     CDS             6914..8269
                     /gene="mpl"
                     /locus_tag="AC16HB_15260"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR5608196.1"
                     /translation="MGGLALLARAAGHRVTGCDANVYPPMSDQLRAAGIDLIEGFDAS
                     QSRMKPDLFVIGNVVSRGNPLMEAILDSGARFVSGPQWVGENLLRGRWVLAVAGTHGK
                     TTTSAMLAWILEHAGMAPSFLIGGVPQDFGVPARTGDSDFFVIEADEYDTAFFDKRSK
                     FVHYFPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRGVPPSGRLIVNGADGALQRVL
                     ARGCWSERDEFNTQDGWHAAAIDEGAAHEFDVRRGDRGFGRLTLPLAGAYNRANAIAA
                     IAAAHHVGVRPDTAIEALASFRGVKRRLEVRGVRAGVTVYDDFAHHPTAIEATIAALR
                     KKFGAARIVAVLEPRSNTMKLGVVKAALPGSLRDADRVFCYGAREGKNALGWDPAEAL
                     APLGAKARSFHEIDALVEAVTAEACAGDHVVVMSNGGFGGIHLKLLNALAARAAQARA
                     A"
     gene            8266..8835
                     /locus_tag="AC16HB_15265"
     CDS             8266..8835
                     /locus_tag="AC16HB_15265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HIR5608197.1"
                     /translation="MILYLHGFRSSPQSKKATQLRRALAARGRGDEFACPQLPASPRA
                     AIEEAQRAVDGVQREQVNLIGSSLGGYYATWLAEQLGCRAVLLNPAIQPYDDLRNFLG
                     SQPVYFSDATIDMKPEYLDELAALDVPRISRPERYFLLAATGDEMIDWRTMVAKYAGC
                     RQRVIEGSDHSISDFATYLDEVLAFCGMA"
     gene            8846..9598
                     /gene="fabG"
                     /locus_tag="AC16HB_15270"
     CDS             8846..9598
                     /gene="fabG"
                     /locus_tag="AC16HB_15270"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR5608198.1"
                     /translation="MRLKGKVSLITGGAQGIGFATAQKFLDEGAIVAICDVRRESVDA
                     AVNELKASESGRIGHVEGFVVDVTNRAQIDAMVDAVRARYGRIDVLVNNAGITQDARL
                     QKMTDEQFDKVIDVNLKGTYNCAQAVVDTMINQGFGVILNASSVVGIYGNFGQTNYAA
                     SKFGVIGFAKTWARELGPKGIRCNAVCPGFIATPILHSIPDKVMAQMTERVPMKRLGR
                     PEEIANVYAFLASDEAGYVNGAVIEVAGGMTV"
     gene            9598..10083
                     /locus_tag="AC16HB_15275"
     CDS             9598..10083
                     /locus_tag="AC16HB_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5608199.1"
                     /translation="MPATKPLPASAQRVQDALGALGLDARVVELTVAARTSQQAADAL
                     GIVVGQIAKSLIFRASNSGRAVLVIAAGDRRVDEAKIAARLGEPIERAMPEFVRAHSS
                     FAIGGVAPLAHAQPMVKFVDASLRRFDVVWAAGGTPHCVFPINPDDLVRISGGVEMET
                     A"
     gene            10095..10391
                     /locus_tag="AC16HB_15280"
     CDS             10095..10391
                     /locus_tag="AC16HB_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608200.1"
                     /translation="METSFLSAVVPPDPSGHSAVVRIALRLAAHMRQGEVIKREFPLA
                     SGLRVLVVFGVGLFMRILGSSAQGLQLGGAVALILGAIALVPMPRGGRAFATGL"
     gene            10396..12363
                     /locus_tag="AC16HB_15285"
     CDS             10396..12363
                     /locus_tag="AC16HB_15285"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR5608201.1"
                     /translation="MSVAHLLFEEDGAFKAGTVLSGTEASYQVELSSGRRTKVKAAHV
                     LLRFEQPAPAALLAGAQRDAESIDLDFLWECAPQQEFGFEDLARDYFGHAPSPVEAAT
                     ILFRLHSAPVYFRRGGRGRFRPAPPDVLKAALAAVERKRQQETLKQHYIDEFAAGRVP
                     MPIAQQAIALLTAPDRNGIEFKALQHAADAAHVSPLRLLLRLGAIPSAYRWHVDSFLA
                     VAFPRGTGFAPELVVPDAAGDLPLAEVDVVSIDDSATTEIDDGFSLQRRDGVMRVGIH
                     IAAPAVAIARGDALDVAARTRMSTVYAPGLKYMMLPQAVVDAFSLNEGRVVPVLSLYV
                     DVDPGTLDILAYETRVERARIAANLRHDQLDAVVTEDRIGAEGSDLPHGELLSLLWRF
                     ARELHARREQARGRPEPLDRIDYTFAIEGDDEDGRVAIRQRRRGAPLDLIVAELMILA
                     NSTWGEWLAAEGRVGIYRSQSLGRVRMSTTPAPHDGIGVDHYAWSSSPLRRYVDLVNQ
                     RQLIAAARGRPAPYEPNDADLFAIVSAFDAAYAAYSDFQYRMERYWCLRWVRQQGIAR
                     TGATVLKEEVVRLDGMPYVARMPGLPALARGRRIEVDILGADEIDTLLELRLHRVLAE
                     TADVLDDEIALQNASETTAVGDAATPVPQGG"
     gene            12426..13307
                     /locus_tag="AC16HB_15290"
     CDS             12426..13307
                     /locus_tag="AC16HB_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5608202.1"
                     /translation="MHTLSQTFTNRTLMTALVVSCGVHALVLAVRFVDPEFLRVRSSD
                     PALEIILVNARSELRSTKADVLAQASLDGGGSNDDGRRTSPLPNLFDIRDGDALEAAR
                     RRSQPIEEDQRRRLTLPGEGDAFALTPRKEAQALEPDSGTQEEIRDRLARMQAEIAKQ
                     ISDYQKRPRRHHFMPSTSEYRFARYVEDWRSWVEKVGNDHYPDEARGRFYGALRMTVV
                     IRKDGTLVEAIVEKGSGSRVLDRAARRIVALAAPFPPFPPEIARDTDILEITRTWTFT
                     NDQFATHSSGGSANVKP"
     gene            13304..14158
                     /gene="aroE"
                     /locus_tag="AC16HB_15295"
     CDS             13304..14158
                     /gene="aroE"
                     /locus_tag="AC16HB_15295"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR5608203.1"
                     /translation="MSSSRIDRYAVVGNPIAHSRSPEIHRMFAKETRQAISYEKLEAP
                     LNGFEDFALGLRDAGYLGLNVTIPFKLDAAKLADELTPRARLAGAVNTLKFDGDTIIG
                     DNTDGIGFVRDVQQRLKFHIKDASVLILGAGGGVRGLIGALLEERPKWIAVANRTHGR
                     AEELADEFGVEAIHFDEVPAEHFDMIINGSASSLNHEAPKIDPEAFDDSTLAYDLVYA
                     AAPTPFMRLAEQGGALKVSDGLGMLIEQAAESFALWRGVRPHCAPVYKELRRLLETEQ
                     TSAAAAVR"
     gene            14202..14906
                     /gene="mtgA"
                     /locus_tag="AC16HB_15300"
     CDS             14202..14906
                     /gene="mtgA"
                     /locus_tag="AC16HB_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR5608204.1"
                     /translation="MSAARAGARVLGWALFAGALVVLALQLWYLGWIVLWHWHNPGET
                     AFMQRELARLRLARPQAALRRQWVSYDRISNHLKRAVIAAEDARFIEHEGVDWEAVQK
                     AYELNLKRGRPARGGSTITQQLAKNLFLSPERSYLRKGQELVITYMIELAWDKRRILE
                     VYLNVAEWGEGVFGAEAAARHYYGISAAQLGAEQSARLAAMLPRPKFYDRYRGSAYLA
                     RRTEAILRYVNDAQLP"
     gene            14939..16441
                     /gene="mgtE"
                     /locus_tag="AC16HB_15305"
     CDS             14939..16441
                     /gene="mgtE"
                     /locus_tag="AC16HB_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR5608205.1"
                     /translation="MSEFEHTRVPPQVDPAATPARDPEEAQRALARVQALLERQRRVE
                     ALVQREQTPTDEKKPLVEQLVHRQHLTELKSIVDRLHPADVAYVLEALPPEERITVWD
                     SVKAEIDGEVLLEVSDAVRESLIAAMDASELVAAAQKLEADELADLAPHLPIDVVDRV
                     SQTLSTKERAQLHAAMSYPEDSVGARMDFELLSIRDDVTLEVVLRFLRRFDELPDHTD
                     QVFVVDREGVLRGALRLDRLLINEPETEVAAVMKTDVLTLAPTDDAGEAAQAFERYDL
                     VSAPVVDAGGRLIGRLTVDKVVDVIREESDQEALAAAGLREEEDLFASVWQSAKNRWL
                     WLAVNLCTAFFASRVIGAFEMTIEKVVALATLMTIVMGISGNSGNQTMTLMIRSIALG
                     QVTGANAKRLIFKELAIAGLNGLVWGGIAGSVAWWIYADSPHGVLIGVTMLIAMTLNM
                     TVGALVGMFVPLALQRLGRDPALGSSVLLTFTTDSMGVFIFLGLATLFFR"
     gene            complement(16404..17051)
                     /locus_tag="AC16HB_15310"
     CDS             complement(16404..17051)
                     /locus_tag="AC16HB_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608206.1"
                     /translation="MRHAIGALAAASIASLALAPAAFAADNSPLGLCPAETARLDGFM
                     RAICDGERALAAGNAAEAAALFNQAAGLARLQATNELAWAGLAAAYCDARDLEHSREW
                     AARFDEARRLWFGELSCDSTFRAQPQPFVRDHMCVDALTADYSFVRTHPDATAAREIT
                     ARLQSVASRVEQHCAQRPAALPAAAPPAKANSNKLSKKKAKKKLSGRRASPARER"
     gene            complement(17072..17290)
                     /locus_tag="AC16HB_15315"
     CDS             complement(17072..17290)
                     /locus_tag="AC16HB_15315"
                     /inference="COORDINATES: protein motif:HMM:NF025005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR5608207.1"
                     /translation="MPIERKPIRRGGIKSAGYDRAKRVLEIEFDNGSVVRHTAVGEEI
                     ARRFLSSSAPASVYKDTIQEEFTIRRVR"
     gene            complement(17300..>17571)
                     /locus_tag="AC16HB_15320"
     CDS             complement(17300..>17571)
                     /locus_tag="AC16HB_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602282.1"
                     /note="catalyzes the decarboxylation of
                     3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-octaprenyl-4-hydroxybenzoate decarboxylase"
                     /protein_id="HIR5608208.1"
                     /translation="DDDVDARDWKEVVWAITTRVDPARDTVMVDNTPIDYLDFASPVS
                     GLGSKMGIDATNKMPGETQRVWGRPIAMDAAVRARIDALWQKLGL"
BASE COUNT         2787 a         5788 c         6010 g         2986 t
ORIGIN      
        1 cccctcccgc cgccctcgct gagcagccgc acccggtcgc ccggcttgaa ctgctggccg
       61 tcgatttcct ggacgatcgc gcgcaggtcg ccgctatcga gcctgactgt gatctcaatc
      121 gctggaacct tcgacgactg attctcgacc gcctgcccga cgatgccgcc ggccaccgcc
      181 ccggcgactg cgccaatcac cgagcccttg cccccgccga cgctgctgcc cgccacaccg
      241 ccgacgattg cgccagcgcc ggtgccgacg ccggtctgac cgcggtcgag cgtcacttcg
      301 cgcacgcttt caacggtggc catccgcacg atctgctcgc gctgcgcctc gccggcgcga
      361 tagacgcttg ccgagcgttg ctgcgtagcg cagccggccg cggtgagaat cgcgagcatg
      421 gcgacgccaa gcgcaacccg cgacccgctc cgtcctgcaa tcattccttc ctcctgaatc
      481 gattcgtagg ctgattcgac cgcgcacggc cgtcgcggtt cggccgagtg taacgagcgg
      541 ctgcgtcgcg gacgtttccg gttgttacgg acttcggatt accacggccg gtagccgaag
      601 gtctccacga agcgcttggc gatcagtgcg cggtccgcac tgtgattctg cgccccgctg
      661 tgctcgatct tcagcgtccc catcacggcg gcaagtcgcc cagtggtctg ccagtccatg
      721 ccattttcga tgccgtaaag caggccggca cgataggcgt caccgcagcc cgtcgggtcg
      781 gagaccgctc gcggcggcgc ggccgggatt tcgagcacct ggccgtcggc gtagatctcg
      841 gagcccttgc cgccgagcgt gacgacgagc gccttgacct tgcgtgcgag cgtgccgaga
      901 tccattccgg tccgctcgat cagcagccgc gcctcgtagt cgtttaccgc gacgtagtgc
      961 gcgcgtgcaa tgagtcgctt cagttcgtca ccatcgaaca tcggcagccc ctggccggga
     1021 tcgaagacga acggcacgcc gtgatcggcg aattgctggg cgtgctgcag catgccatcg
     1081 cgcccgtccg gcgcgatgat gccgaggcgg actacgcctg caccgggaac cgcgttcaag
     1141 tgcgagtgcg acatggcgcc cgggtgaaag gcagtgatct gattgtcgtc aaggtcggtg
     1201 gtgatgaaag cctgggccgt ataggtgcct ggcacgtgcc ggacgtggcg ccggtcgatg
     1261 ccgagcgcgt cgagccgatc gaggtacggc tgagcgtcgt ctccgaccgt agccatcggc
     1321 aacggcgttc cgccaagcat gcggagactg taggcgatgt tgccagcaca gccgccgaac
     1381 tcgcgccgca gttgcggcac caggaacgcg acgttcagga tgtgcacctg ctcgggaaga
     1441 atatggtcct tgaatcttcc ttcgaagacc atgatcgagt cgtatgcgat cgaaccgcag
     1501 atcagcgtgg acatgagggt gcgcgggccg gttctaaaga tagaaaggat agacaacgaa
     1561 gccggcaacg ttcagtccgc gcgcctcgaa ggtgatccgt acgatcatgt ccgcaccggc
     1621 ctgcaggccg tccgcgagcc gcgcggctgc ttcgctcggc ccgaggtagt cgatcgggcc
     1681 gaagatccgg cgcgcgacgg cgcggttctg ggtgtcggtc agcgtgactt cgagcgctgg
     1741 aagggccagc gtgatctcgg cgcggttacg caccacggct gtcagctcga gcgcattcgt
     1801 gcccggcagc gactgcagtt cgctgcccac gatggccagt agttcaccgt gcatcggcca
     1861 gccgaccgca cagtgcatca cctggcacac ggcgaccagc accccacgcg tggctggcag
     1921 ttgggctgcg atctcggtgc ggtacatcag cacgagttgc agcgccagtg ccagcgcggc
     1981 cagcgccgcg ccgagcgcgt agaccagcgt cacgacgcgt cggcgcggcg tgcttccctc
     2041 gtgcaggaac gcgggtgcac tcgtgtccgg cacgacagct tcgtcgacca gttccgacgc
     2101 ggccccgtcg acttcagccg aacgatccgc tggctgctcc tcgctgtcga tcgcatcgat
     2161 tgcctcggcg tcgtgcatct cgtctgccgc cggcacgatt ggtcggcttt caacgtgtga
     2221 ttcatcctga cggcggtcga tcgcgaactc ggcattcgat tcgtctggca cggttgccgc
     2281 agcaatcgtt tcgtcccgtc gatccggcac gctgggccct tcgaccggca cggtcgcagg
     2341 ctgtccggcg aactcggccg gcggaacgac cggcggcggc gccggcgtcg gttcgattgt
     2401 tgccggcgcg ggtgtggcgg cggggcgcac ggacgcatcg tccagatagc tcagcgttcc
     2461 gatggcgtcg aacacatggc cgcaaccgcc gcagcggaca aggccaccgc gcaacttcag
     2521 ctggtccgcg acgacgcgaa acagcgcgtg gcagctcggg caacgggttg cgagcgccat
     2581 ccgtctcagc cggtgcgttg agtgacgatg cagcaccagc cgtcgtcgac gcgccagacg
     2641 cgcagcgcga gttccgaaca aacgcgcgcg tacgcgtcga tcacctgcgg tgcctgccgt
     2701 tcgaggatgc cggacagcac gatctgcccg ccgtcggcga gccgagcgac cagcgctggc
     2761 gcaagcagga ttagcggatt ggccaggatg ttggcgagaa tcacgtcgaa aaggccgcgt
     2821 cccaattcct gcggggcagt gtagcgggcc gtgacctgat tgattgcgct gttcgtacgc
     2881 gcggcctgca acgcctgcgg atcgacgtcg gttccgacca cttcgcgcgc gccgagtttc
     2941 gcggcggtga tcgcgaggat gccggagccg cagccatagt cgagcacgcg cgcgttcgcc
     3001 agtcgcgcct gcgcgagcca gctcaggcac agccgcgtgg tcggatgcgt gccggtgccg
     3061 aacgccacgc ccggatcgag ccggatgttg atcgcatccg cgtccggcgg ttcgtgccag
     3121 gtcggcacga tccacagacg gtcgctgagc cgcgttggcg cgaactgcgc ctgtgtcagc
     3181 cgcacccagt cggcgtcgcc gacttcgcgc gtcgcgatga tcgccggtgg cggccgatcg
     3241 atcgaggctg cggcactggc cagcatttcc gccgcggtcg ccgcggcatc gaccatgacg
     3301 atcaggcggc tccggcgcca ggccggccgc gcgggctcga gacccggttc accgaagagc
     3361 ggctgctcgc cttccagatc ggcgtcggca tcttcgaccg cgaccgacag tgcgccggca
     3421 tcgagcagcg cgtccgacag cgtgtcgacg tcctccgcgt cgacgatgac cacgacttcg
     3481 cgctgcacgt cgtcgtccgg cccgcgctca aggctggttg agctggcgcg cggcaagctt
     3541 cttttcgagg tagtggatgc tcgtgccgcc gaggacgaat ttctcgtcga gcatcagctc
     3601 gcgatgcagc gggatgttgg tcgaaatgcc ctcgacgatc atctcggaca gcgcgacccg
     3661 catccgcgcc agcgcctggc tgcgcgtatc gccgtagcag atcaacttgc cgatgagcga
     3721 gtcatagtgc ggcggcacga agtagccgga atatgcgtgc gaatcgacgc gcacgcccgg
     3781 tccgcccggc ggatgccatg ccgtgatgcg gccagggctg ggcgtgaact tgtacgggtc
     3841 ctcggcgttg atgcggcact cgatcgcggc gccgcgcagc gcgatgtcgc gctggcggaa
     3901 cggcagtctc tcgccggccg cgatgcggat ctgctgctgc acgatgtcga ttcccgtcac
     3961 catctcggtg accggatgct cgacctgaac gcgggtgttc atttcgatga agcagaactc
     4021 gccgttctcg taaaggaatt cgaacgtgcc ggctccgcga tagccgatct tgcggcacgc
     4081 ctccgcgcag cgatcgccga tgcgctcgac cagcttgcgc gcgatgccgg gcgccggcgc
     4141 ctcttcgatg atcttctggt gccgccgctg catcgagcag tcgcgctcgc cgagccacac
     4201 cgcgttgcga tgctggtcgg ccagcacctg gatctcgatg tggcgcgggt tctcgaggaa
     4261 tctctccata tagacggccg ggttgccgaa tgccgactgc gcctcctgtc gagtcatgct
     4321 gaccgcgttc accagcgcgg cctcggtgtg gaccacgcgc atcccgcgcc cgccgccgcc
     4381 gcccgcggcc ttgatgatca ccgggtagcc gatcgcacgc gcgatcttga caatctcgcg
     4441 cggctcgtcg ggcaacgtgc cgtcggagcc cggcacggtc ggcacgttcg ccgcgatcat
     4501 cgccttcttg gccgacacct tgtcgcccat cagccggatc gactcggggc gcgggccgat
     4561 gaacgcgaag ccgctcttct ccacgcgctc ggcgaagtcg gcgttctcgg acagaaagcc
     4621 gtagccggga tggacagcct cggcgtcggt gacctcggcc gcgctgatga tcgccggaat
     4681 gttcagatag ctgtccctgg agggcggcgg accgatgcac accgactcgt cggcaagctt
     4741 cacgtacttg gcgtccgtgt cggcggtcga atggaccacc acggtcttga tgccgagttc
     4801 acggcaggtg cgttggacac gcagcgctat ctcgccgcgg ttcgcgatca gtactttgcc
     4861 gaacattgcc gctactcgat cacgaacagc ggctggccgc gctggctgtg gccgctcgcg
     4921 tgtgctcgct taacgttcac ttcgttcacg tgagcctccg ccgaaacggc caatcattca
     4981 atgacgaaca atggttggcc gtattccacg ggctggccgt tctcaaccag gcattccttc
     5041 acgacgcccg ccacctcggc ctcgatttcg ttcagcagct tcattgcctc gatgatgcac
     5101 agcgtgtcgc cgggtttcac ggcctggccg acgtcgacaa atgggttcgc gcctggactc
     5161 gacgcgcgat agaacgtgcc caccatcggc gatttcacga tctggccctt gagcggctcg
     5221 gcgcccggca tggagggggc cactggcgga gttgtcgggg tggcggccgc ggcaggctgc
     5281 gctgccatcg ccgcctgcgc cgcagttacg gctggcagcg ccgcaaccgg catcggcggt
     5341 ggtgggccgt gcttgacaat gcggaccttg tcctcgccct cggtgacctc gatttcggaa
     5401 atgccggatt cggcgaccag atcgatgagc gtcttgagct tgcgaagatc catgccctct
     5461 cccacgaacc ggcgggttca gcgcgcgcgg cgacacagcc catactgacc gatgacggtg
     5521 gcttggtgcg aagttttgtt caggattcgc taccgttgcc gagaaatggg agcggattgt
     5581 ccgcggatcg gtgctttttg tccattcgga tcaaccgccg gcggccaact gcgcattgag
     5641 ccaggctctg agttgttcgg gccggatctg cccgacgtgg cgctgttcga tcgctcccga
     5701 tcgcgcgacc agcacggtat acggcagcac gccggccagg tttccgagtg tgcgggcgag
     5761 ttcgctgccg tcggatcccg cgaccagcag cggcagtccg atgccgagct tgtcgcggaa
     5821 ggcgcgaatc ctgtccgcgc tgtcgatccc gataccgatc acctccacgc cgtgcgcgcc
     5881 gaattcgtcg cgtacgcgat ccagcgccgg catctcctcc acgcacggcg cgcaccaggt
     5941 agcccagaag ttgaccacta acagcgttcc gcgccattgc gccagcggcc gtgcgatgcc
     6001 gtctgcgtcg gtgtaagtcg ccgcgaagag cgcagccgca ccggcggccg acccgctgcg
     6061 atgcgcttcc cgccacaacg ccacaccgat tcccattgca agagcgctgc tcgcggtcgc
     6121 ggccaggagc cagcggcgcc gcacgtcatg cctccggatc gcgcgcggca atcagcgcac
     6181 cgagcatcgt cgcgttcgcg cggccggcgg cttcgactgg atgcaggccg ggcgcagtgg
     6241 agcggtactt cggcgcgacg cggagcgcgt ccgtgtcgag cacgtcgatg acgacaccca
     6301 cgcgcggcgg cattccgcgc gtgcgcgcgg gccgtacgac caagtgcagg acctcgagcg
     6361 gttcgccagg ctgccggcca ccttcgatca cgtcgtactc cacgccttgt tcgagcagaa
     6421 acatctcgac atccttcgcg ctgtccacaa agacctgcag gtggatgtcc gagtgctcgg
     6481 tcgccgtgcc attcagcacc gcgcccacga tgtgcgggcg gaagcggtcg agccgctgca
     6541 tcagctgtag cgccagctgc cgcagggccg cgagtaccgc cgcgtgccgc ggcaggaagg
     6601 tcgaaagatg gcggcgcaac tccatttcga tctgcgcgtt gtcgggcaat gcgcctcgag
     6661 cggcgacggc attgccaagg atcgcctgcg cggccttctg cttggcggtc gcatagtcga
     6721 ggccgtcctc ggcgatcagc cgagctgcgg cggccgccag ttcggtgcgc aggtcgtcgc
     6781 gttcgggatg gctcatggtg tcgggcatat ggcggaacca agagtctaaa catccgaccg
     6841 tacaatcgcg tgtttgcgtt ctcgttccgg cagtcccgtg catctccata tcctcggcat
     6901 ctgcggcacc ttcatgggcg ggctggcgct gcttgcacgc gcggccggcc atcgtgtgac
     6961 gggctgcgac gccaacgtgt acccgccgat gagcgatcag ctccgcgcgg ccggcatcga
     7021 tctcatcgag ggattcgacg ccagccagtc gcggatgaaa ccggatctgt tcgtgatcgg
     7081 caacgtcgtt tcgcgcggca atccgctcat ggaagcgatc ctcgattcag gcgcgcgctt
     7141 cgtctcgggg ccgcagtggg tgggcgagaa cctgctgcgc ggccgctggg tgctggcggt
     7201 tgccggcacg cacggcaaga cgaccaccag cgcgatgctt gcctggatcc tcgaacatgc
     7261 cggcatggca ccgtcgttcc tgattggtgg cgtaccgcag gacttcggcg tgcccgcgcg
     7321 cacgggcgat tcggatttct tcgtgatcga ggccgacgag tacgacacgg ctttcttcga
     7381 caagcgcagc aagttcgtgc actactttcc gcgtaccgcg gtgttgaaca acctcgagtt
     7441 cgatcacgcc gacatcttcc ccgacctcgc ggcgatcgag acccagttcc accatctcgt
     7501 gcgtggggtc ccgccgtcgg ggcggctgat cgtcaacggc gccgacggcg cgttgcagcg
     7561 tgtgctcgcg cgcggctgct ggtccgagcg ggatgaattc aacacgcagg acggctggca
     7621 cgcagcggca atcgacgaag gcgccgcgca cgagttcgac gtgcgacgcg gcgatcgagg
     7681 tttcggtcgg ctgacattgc cgctggcggg cgcatacaac cgcgccaacg caatcgccgc
     7741 gatcgcggcc gcgcaccacg tcggcgtgcg tccagacacc gcgatcgagg cgcttgccag
     7801 cttccggggc gtgaaacgac gactcgaagt gcgcggcgtg cgcgcgggtg tcaccgtcta
     7861 tgacgacttc gcgcaccatc cgaccgcgat cgaggcgacg atcgcggccc tgcgcaagaa
     7921 gttcggcgcc gcgcgaatcg tggcggtgct ggagccgcgt tcgaacacga tgaagctcgg
     7981 cgtggtcaag gcggccctgc ccggcagtct gcgcgatgcc gaccgggtgt tctgctacgg
     8041 cgcgcgcgaa ggtaagaacg cgctcggctg ggatccggca gaagcactcg cgccgctcgg
     8101 cgccaaggcg cggtcgttcc acgagatcga cgcactggtt gaagcggtga cggcagaagc
     8161 gtgcgcgggc gaccacgtgg tcgtgatgag caacggcggc ttcggcggta ttcatctgaa
     8221 gctgttgaat gcgctggcgg cacgcgcagc gcaggcgagg gccgcgtgat cctgtatctg
     8281 cacgggttcc gctcgagccc gcagtcgaag aaggcgacgc agctgcgccg cgcgctggca
     8341 gcgcggggcc gcggtgacga attcgcctgc ccgcagctgc cggcgtcgcc gcgcgcggcg
     8401 atcgaggagg cgcagcgcgc agtcgatggc gtgcagcgcg aacaagtgaa cctgatcggt
     8461 tcgtccctcg gcggctacta cgcaacgtgg ctggcagaac agctcggctg ccgtgccgtg
     8521 ctgctgaacc cggcgattca accgtatgac gatctgcgca acttcctcgg ctcgcagccg
     8581 gtgtactttt ccgacgcgac gatcgacatg aagccggagt acctggatga gcttgcggcg
     8641 ctggatgtgc cgcgcatctc gcgaccggaa cggtattttc tgctggccgc gaccggggac
     8701 gaaatgatcg actggcggac catggtcgcc aagtacgcgg gctgccgcca gcgcgtgatc
     8761 gaaggctccg atcattcgat atccgacttc gccacttacc tcgacgaggt gctggcattc
     8821 tgtggcatgg catagggaga gagcagtgag attgaaaggc aaggtcagcc tgattaccgg
     8881 gggcgcgcaa ggcatcggct tcgccaccgc gcagaagttc ctcgacgagg gcgcgatcgt
     8941 ggcgatctgc gacgtgcggc gcgaatcggt cgacgccgcg gtcaacgagc tgaaggcgtc
     9001 cgagtcgggc cggatcggcc atgtcgaagg gtttgtcgtc gacgtgacca atcgcgcgca
     9061 gatcgacgcg atggtcgatg ccgtgcgggc gcgctacgga cgcatcgacg tgctggtcaa
     9121 caatgccggc atcactcagg acgcgcggct gcagaagatg accgacgagc agttcgacaa
     9181 ggtgatcgac gtgaatctca agggaacgta caactgcgcg caggcggtgg tcgatacgat
     9241 gatcaaccag gggttcggcg tcattctgaa tgcttcgagc gtggtcggca tctacggcaa
     9301 cttcggccag accaactacg cggcgagcaa gttcggtgtg atcggttttg ccaagacctg
     9361 ggcgcgcgaa ctggggccga agggcattcg ctgcaacgcg gtctgccccg gcttcatcgc
     9421 cacgccgatc ctgcactcga tcccggacaa ggtgatggca cagatgaccg agcgcgtgcc
     9481 gatgaagcgg ctcggccgcc ccgaggagat cgcaaacgtc tacgcgttcc tcgcctcgga
     9541 cgaagcaggc tacgtgaacg gcgcggtgat cgaggtcgcc ggcggcatga cggtctgatg
     9601 cccgccacca agccgctgcc ggcttcggcg cagcgcgtcc aggatgcgct cggcgcgctc
     9661 ggcctcgacg cgcgggtggt cgaactcacg gtggcggcgc gcacctcgca gcaggcggcc
     9721 gatgcgctcg gcattgtcgt cggtcagatt gccaagtcgc tgatcttccg cgcgtccaac
     9781 tcgggccgcg cggtgttggt gatcgccgcg ggcgaccgcc gagtcgacga agcaaagatc
     9841 gcggcgcggc tcggcgagcc gatcgagcga gccatgccgg agttcgtgcg agcgcactcc
     9901 agcttcgcga tcggcggcgt ggcgccgctg gcgcacgcac agccgatggt gaagttcgtc
     9961 gatgcgtcgc tgcggcgctt cgacgtcgtg tgggcagcgg gcggcacgcc gcactgcgtg
    10021 ttcccgatca atcctgacga cctggtgcgc atcagcggcg gcgtcgagat ggaaacagcc
    10081 tgacggcgga caggatggaa acgtcgttcc tctcggcggt cgtgccgccc gatccgtccg
    10141 gccacagcgc ggtcgtccgc atcgcactgc gcttggcggc acacatgcgg cagggcgagg
    10201 tgatcaagcg cgaatttccg ctggcgtccg gcctgcgcgt cctcgtcgtg ttcggtgtcg
    10261 gtctgttcat gcgcattctc ggctcgtcgg cgcaggggct gcagctcggt ggtgccgtgg
    10321 cgctgatcct gggggcgatt gcgctcgtgc cgatgccgcg aggcggccgc gcattcgcaa
    10381 ccggcctgta attcgatgtc ggtggcacac ctgctgttcg aggaggatgg tgcgttcaag
    10441 gccggcaccg tattgagcgg cacggaagcg tcgtaccagg tcgagttgtc gtccggcagg
    10501 cgcaccaagg tcaaggcggc gcacgtgttg ctgcgtttcg agcagcccgc gccggcggcg
    10561 cttctggcgg gcgcgcaacg cgacgcggag tcgattgatc tggattttct gtgggaatgc
    10621 gcgccgcagc aggagttcgg cttcgaggac ctcgcgcgcg attacttcgg ccacgcaccg
    10681 agccccgtcg aggcagcgac aatcctgttc cgcctgcata gcgcgccggt gtacttccgc
    10741 cgcggagggc gcggacgttt tcgacccgcg ccgcccgatg tgctgaaggc tgcgctggcg
    10801 gcggtcgagc gcaagcgtca gcaggagacg ctcaagcagc attacatcga tgaattcgcg
    10861 gcaggccgcg tgccgatgcc gatcgcgcag caggcgatcg cgctgcttac cgccccggat
    10921 cgcaacggca ttgagttcaa ggcgctgcag cacgcggccg acgccgcgca cgtgtcgccg
    10981 ctgcggctgc tgctgaggct gggtgcgatt ccgtccgcgt atcgctggca cgtcgacagc
    11041 ttcctcgcgg tggcatttcc gcgtggcacc ggcttcgcgc cggagttggt ggtgccggac
    11101 gcagccggcg atctgccact tgccgaggtc gacgtcgtgt cgatcgacga cagcgcgacg
    11161 accgaaatcg acgatggctt ttcgctgcag cggcgcgacg gcgtcatgcg cgtcggcatc
    11221 cacatcgcag cgccggcagt ggcgatcgcg cgcggcgacg ccctcgatgt ggctgcgcgc
    11281 acgcgcatgt cgaccgttta tgcgccggga ctgaagtaca tgatgctgcc gcaggcggtc
    11341 gtcgacgcat tctcgctgaa cgagggccgc gtcgtgccgg tcttgtccct gtacgtcgac
    11401 gtcgacccgg gtacgctcga catcctggcc tacgagacgc gcgtggagcg cgcgcgtatc
    11461 gcggccaacc tgcgccacga tcagctcgat gcggtcgtta ccgaggaccg catcggtgcc
    11521 gaaggctccg acctgccaca tggcgagctg ctgtctctgc tttggcgctt tgcgcgggag
    11581 ctgcacgcgc ggcgcgagca ggcgcgcggg cgtcccgagc cgctcgaccg catcgattac
    11641 acgttcgcga tcgaaggtga tgacgaggac gggcgcgtgg cgatccggca gcgccggcgc
    11701 ggcgcgccgc tcgatctgat cgttgccgag ttgatgatcc tcgccaacag cacctggggc
    11761 gaatggctcg cggccgaagg ccgtgtcggg atctatcgct cgcagtcgct tgggcgggtg
    11821 cggatgagca cgacgccggc gccgcacgac ggcatcggcg tcgatcacta cgcgtggagt
    11881 tcgtcgccgc tgcgtcgcta cgtcgatctg gtgaatcagc gccagctgat tgcggccgcg
    11941 cgcggccggc cagcgccgta cgaaccgaac gatgcagact tgttcgcgat cgtctccgcg
    12001 ttcgacgctg cctacgccgc gtattccgat ttccagtatc gaatggagcg ctactggtgc
    12061 ctgcgctggg tacggcagca gggcatcgcg cgcaccggcg ccacggtgct gaaggaggag
    12121 gtggtgcggc tcgacggcat gccctacgtc gcgcgcatgc cgggcttgcc cgcgctggcg
    12181 cgtggtcgcc gcatcgaggt cgacatcctg ggcgccgacg agatcgacac cttgctggag
    12241 ctgcgactgc accgcgtgct ggccgaaacg gccgacgtgc tcgacgacga gattgcactg
    12301 cagaacgcaa gcgaaacgac ggcggtcggc gacgccgcga cgccggtccc gcaaggcggg
    12361 taatccgcga ctgggcacgc gcagcgcgct ggatacaatc gccgcaccgt tgcgcacacc
    12421 ggccgatgca tacgttatcg cagaccttca cgaatcgcac gctgatgacg gcgctcgtcg
    12481 tctcatgcgg tgtgcacgcg ctcgtgctcg cggtgcgatt cgtcgatccc gagttcctgc
    12541 gtgtgcgttc cagcgacccg gcgctcgaga tcatcctcgt taacgctcgc agcgaactgc
    12601 ggtcgacgaa ggccgacgtg cttgcgcagg ccagcctcga cggcggcggg tcgaacgatg
    12661 acgggcgccg cacttccccg ctgccgaatc tctttgacat ccgcgacggc gacgctcttg
    12721 aggccgcgcg gcgtcgatcg cagccgatcg aagaagacca gcgacgccga ctgacactgc
    12781 ccggcgaagg agacgcgttc gcgctgacgc cgcgcaagga ggcgcaggcg ctcgagccgg
    12841 acagcggcac gcaggaagaa attcgcgatc ggctggcgcg catgcaggcc gagatcgcca
    12901 agcagatctc cgattaccag aagcggccgc gccggcatca cttcatgccg agcacatcgg
    12961 agtaccgttt cgcacggtac gtcgaggact ggcgcagctg ggtggagaag gtcggcaacg
    13021 atcactaccc cgacgaggcg cgcggccgct tctacggcgc gctgcgcatg acggtcgtga
    13081 tccgcaagga cggcacactc gtcgaggcga tcgttgagaa gggctcggga tcgagggtgc
    13141 tcgaccgcgc ggcgcgccgt attgtcgcct tggcggcacc gtttccgccg ttcccgcccg
    13201 agatcgcgcg cgacaccgat atcctcgaaa taacgcggac ctggactttc accaacgatc
    13261 agttcgccac ccacagcagc ggtgggtctg ctaacgtgaa gccatgagca gcagcaggat
    13321 cgaccgctac gccgtcgtcg gcaacccgat cgcgcacagc cgctcgcccg aaatccatcg
    13381 catgttcgcg aaggagaccc gacaggcgat cagctacgaa aagctcgaag cgccgttgaa
    13441 cggcttcgag gacttcgccc tcgggctgcg cgacgccggc tacctggggc tgaacgtcac
    13501 gattccgttc aagctcgacg cggccaagct cgccgacgag ctgacgccgc gcgcgcgcct
    13561 ggcgggcgcg gtcaacacgc tgaaattcga cggcgacacg atcattggcg acaacaccga
    13621 cggtatcggc ttcgtgcgcg acgtgcagca gcggctgaag ttccacatca aggatgcaag
    13681 cgtgctgatc ctcggcgccg gcggcggcgt ccgcggcctg atcggcgcgc tgctcgagga
    13741 acggccgaag tggatcgcgg tggccaaccg cacgcacggc cgtgccgagg aactcgccga
    13801 cgagttcggt gtcgaggcga tccacttcga cgaagtcccg gccgagcact tcgacatgat
    13861 catcaacggc agcgcaagca gcctgaatca cgaagcaccg aagatcgatc ccgaggcgtt
    13921 cgacgactcc acgcttgcct atgacttggt gtacgcggct gcgccgacac cgttcatgcg
    13981 gctagcggag cagggcggcg cgctgaaggt cagcgacggc ttgggcatgc tgatcgagca
    14041 ggccgccgag tcgttcgcgt tgtggcgcgg cgtgcgtccg cactgcgcgc ccgtgtacaa
    14101 ggagctgcga cggctgctcg agaccgagca gacctccgcg gcggcggccg tgcgctgagc
    14161 gctgccgctc ggtcccgcat cgcttcgatg ggaatgctgg gatgagcgcg gcgcgagccg
    14221 gcgcccgcgt tctaggctgg gcgctgttcg cgggcgcgct cgtcgtgctg gcgctgcagc
    14281 tttggtacct cggttggatc gtgctttggc attggcacaa cccgggcgag actgcgttca
    14341 tgcagcgcga gctggcgcgg ttgaggctgg cacggccgca ggccgcgctg cgccggcagt
    14401 gggtctcgta cgaccgcatt tccaatcacc tgaagcgcgc ggtgatcgcg gccgaggacg
    14461 cgcgcttcat cgagcacgag ggtgtggact gggaggctgt ccagaaggct tacgaactga
    14521 atctgaaacg tggccgtccg gcgcggggcg gctcgaccat cacgcagcag ctggcgaaga
    14581 acctgttcct gtcgcccgag cgcagctact tgcgcaaggg acaggagctc gtcatcacgt
    14641 acatgatcga actcgcgtgg gacaagcgcc gcatcctcga ggtctatcta aacgtcgccg
    14701 agtggggcga aggcgtgttc ggcgccgagg ccgcggcgcg ccactactac ggcatctcgg
    14761 ctgcgcagct cggtgccgag caatcggcgc gactggcggc gatgctgccg cgcccgaaat
    14821 tctatgaccg ctatcgcggc tcggcctacc tcgcgcgccg caccgaggca atcctgcgct
    14881 acgtcaacga cgcgcaattg ccgtagccgc tgcggtgcgc gcctagaatc gcgccgccat
    14941 gagtgagttc gagcacaccc gggttccgcc gcaggttgat ccggcggcaa cgcccgcgcg
    15001 cgatccggaa gaggcgcagc gtgcgctggc gcgcgttcag gcgttgctgg agaggcagcg
    15061 ccgcgtcgag gcgctggtcc agcgcgagca gacgccgacc gacgagaaga agccgctggt
    15121 cgagcagctt gttcatcgcc agcacctgac cgaactgaaa tcgatcgtcg atcggctaca
    15181 tccggccgac gtcgcctacg tgctcgaggc cctgcccccc gaggagcgga tcacggtctg
    15241 ggacagcgta aaggccgaga tcgacggcga ggtgctcctc gaggtgtcgg acgcggtgcg
    15301 cgagtcgctg attgctgcga tggatgcgtc cgagctggtc gccgccgcgc agaagctcga
    15361 ggccgacgaa ctggcggacc tggctccgca tctgccgatc gacgtggtcg accgggtgtc
    15421 gcagaccctg tcgaccaagg agcgcgcgca gctgcacgcg gcgatgagct acccggagga
    15481 ctccgtcggc gcgcgcatgg acttcgagct gttgtcgatc cgcgatgacg tgacgctcga
    15541 ggtggtgctg cgcttcctgc gccgcttcga tgagctaccg gaccacaccg accaggtgtt
    15601 cgtggtcgac cgcgaaggcg tgctgcgcgg cgcgctgcgg ctggaccggc tgctgatcaa
    15661 cgagccggaa accgaagtgg cggcagtgat gaagaccgac gtcctgacgc tggcgccgac
    15721 cgatgacgcg ggcgaggcgg cacaggcgtt cgagcgctac gacctggtat cggcgccggt
    15781 ggtcgacgcc ggcgggcgac tgatcggccg gctgaccgtc gacaaggtgg tcgacgtgat
    15841 tcgtgaggag agcgaccagg aagcactcgc cgccgccggt ctgcgcgagg aagaggacct
    15901 gttcgccagc gtgtggcagt cggcgaagaa ccgctggctc tggctggccg tgaacttgtg
    15961 cacggcgttc ttcgcgtcgc gtgtgatcgg cgcgttcgag atgacgatcg agaaggtggt
    16021 cgcgttggcg acgctgatga cgatcgtcat gggaatttcc ggcaactccg gcaaccagac
    16081 gatgacgctg atgatccgct cgatcgcact cggtcaggtc actggtgcca atgcgaagcg
    16141 gctgatcttc aaggagctgg cgatcgccgg gctcaacggc ctggtgtggg gtggcattgc
    16201 cggttcggtg gcgtggtgga tctacgccga ttcgccgcat ggcgtgctga tcggcgtaac
    16261 catgctgatt gcgatgacgc tgaacatgac ggtcggtgcg ctggtcggaa tgttcgtgcc
    16321 gctggcgctg cagcgccttg gccgcgatcc agccctgggc tcgtcggtgc tgctgacctt
    16381 caccaccgac agcatgggcg tctttatctt tctcgggctg gcgacgctct tcttccgctg
    16441 agcttctttt tcgccttctt cttcgacagc ttgttggagt tcgccttggc aggtggcgcg
    16501 gcggcaggca gggccgccgg ccgctgcgcg caatgctgct cgacgcggct cgccacgctc
    16561 tgaagccgcg ccgtgatctc gcgcgccgcc gtcgcatcgg gatgcgtgcg gacgaagctg
    16621 tagtcggcag tcaacgcgtc gacgcacatg tggtcgcgca cgaacggctg cggctgggct
    16681 cgaaacgtgc tgtcacagct cagttcgccg aaccagagcc ggcgcgcttc gtcgaaacgg
    16741 gccgcccact cgcggctgtg ctcgaggtcc cgcgcgtcgc agtaggcggc ggccagtccg
    16801 gcccacgcga gttcgttagt tgcctgcagc cgcgccagtc cggcggcctg gttgaacagc
    16861 gcggccgctt cggcagcgtt tcctgccgcc agtgcgcgtt cgccatcgca gatcgcgcgc
    16921 atgaaaccgt cgagccgcgc ggtttccgcc gggcacaggc caagcggcga gttgtcggcg
    16981 gcaaacgccg cgggggcgag cgcgagcgaa gcgatcgacg cagcggcgag cgcaccgatc
    17041 gcatgtcgca tcgccttcgg ccaaccctgg ctcaacggac gcgccggatc gtgaactcct
    17101 cctggatcgt gtccttgtac acgctggccg gagcactgct cgacaggaag cggcgcgcga
    17161 tttcctcgcc gacggcggtg tggcggacca ccgagccgtt gtcgaactcg atttcgagca
    17221 cgcgcttggc gcgatcgtaa ccggccgact tgataccgcc gcggcggatg ggcttgcgtt
    17281 cgatcggcat ccgttcctcc tacagaccga gtttctgcca gagcgcgtcg atgcgcgccc
    17341 tgaccgccgc gtccatagcg atcggccggc cccagacacg ctgcgtctcg ccgggcatct
    17401 tgttggtggc gtcgatcccc atcttgctgc cgagtcccga caccggcgac gcgaagtcca
    17461 ggtagtcgat cggcgtgttg tcgaccatca ccgtgtcgcg agcggggtcg acgcgggtcg
    17521 tgatagccca gacgacttcc ttccagtcgc gtgcgtcgac gtcgtcgtcc a
//
LOCUS       DBNHBY010000636         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1194598, whole genome shotgun sequence.
ACCESSION   DBNHBY010000636 DBNHBY010000000
VERSION     DBNHBY010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1194598"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2075
                     /locus_tag="AC16HB_15325"
     CDS             <1..2075
                     /locus_tag="AC16HB_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR5608209.1"
                     /translation="EVRDDVAVLQIDNPPVNGLSHATRNGLRAGLQHALDNPRVVAIV
                     ITGTGTVFSGGADIKEFNTPAALAEPNLHQVIDAVEGAYKPVIAAINGVCMGGGLELA
                     LACHYRVATPNASIALPEVKLGLIPGAGGTQRLPRAVGADKALRMIATGEPIGADDAL
                     KHGLVERVVPNTTFQGVLDFAHEVADTMPLPKLRHKAVALPPGIDAASFFAGARVQVG
                     REARGLTAPLKCVDAVEAAVLKPFDEGLSFERQTFLELVQSRESKALRHAFFAEREAG
                     KISDVPEGTPTRAIGSAAIIGFGTMGGGIAMSFANAGIPVTVMEMNQAGLDKGVATCR
                     ANWEATARKGRLTQEQVERRMALLKPTLAIEDLRDADIVIEAVFEDLAVKQDLFRRLD
                     RVMKPGAILATNTSTLDVDAIAAVTRRPQDVLGTHFFSPANVMRLLEVVRAKRTGKDV
                     LASVMKLARRLKKVAVVSGVCDGFIGNRMLEQYVRQSLFLVDEGASPQKIDAALTKFG
                     MAMGPFAMYDMAGMDIGYAIRQRRYKEKPHITYSRIGDRVVELGRLGQKTGKGWYRYE
                     PGNRAPIPDPEIDALIDAYRKEIGIKPRAISDEEIVQRCIFALVNEGARILDEGIAQR
                     ASDIDVAYLAGYGFPAATGGPMFHAQTVGFDKVIKTMERFAGNAHADPAFWNPAPRLR
                     RAAVEGHW"
     gene            2083..>2312
                     /locus_tag="AC16HB_15330"
     CDS             2083..>2312
                     /locus_tag="AC16HB_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5608210.1"
                     /translation="MIEPRLLRFLLYDWLDAGSLTRRARYAEHSRETFDAALEAAARL
                     AAREFAPHNRAADLNEPRLVGEQVQLLPDIKRA"
BASE COUNT          396 a          772 c          807 g          337 t
ORIGIN      
        1 tcgaggtgag ggacgacgtc gcggtactgc agatcgacaa tccgccggtc aacggcctca
       61 gtcatgcgac gcgcaacggc ctgcgcgccg gcctgcagca cgcgctcgac aatccgcgcg
      121 tcgtggcgat cgtgatcacg ggcacgggaa ccgtgttctc gggcggcgcc gacatcaagg
      181 aattcaacac cccggcagcg ctggcggagc ccaacctgca ccaggtgatc gacgcggtcg
      241 aaggcgccta caaaccggtc atcgccgcga tcaacggtgt gtgcatgggc ggaggactcg
      301 aactggcgct cgcctgtcat taccgcgtcg ccaccccgaa tgcgtcgatc gcgctgcccg
      361 aggtcaagct cgggctgatc cccggcgcgg gcggaacgca gcggctgccg cgcgcggtcg
      421 gcgccgacaa ggcgttgcgc atgatcgcga ccggcgagcc gatcggcgcc gacgacgcgc
      481 tgaagcacgg cctggtcgag cgcgtggtgc cgaacacgac cttccagggc gtgctcgact
      541 tcgcacacga ggtcgccgac acgatgccgc tgccgaagct gcgccacaaa gcggtcgcgc
      601 tgccgccggg aatcgacgcg gcgtcgttct tcgccggcgc gcgggtgcag gtcgggcgcg
      661 aggcgcgcgg gctgaccgcg ccgctgaaat gcgtcgatgc ggtcgaggcc gcggtgctga
      721 agcctttcga cgaggggttg tccttcgagc ggcagacgtt cctcgaactg gtgcagtcgc
      781 gcgagtcgaa ggcgctgcgt catgcgttct tcgccgagcg cgaggcaggc aagatttccg
      841 atgtgcccga aggcacgccg acgcgagcga tcggcagcgc ggcgatcatc ggcttcggca
      901 cgatgggcgg cggcatcgcg atgagcttcg ccaacgccgg catccctgtc acggtgatgg
      961 agatgaacca ggccgggctc gacaagggcg tggcgacctg ccgcgccaac tgggaggcga
     1021 cggcgcgcaa agggcgattg acgcaggagc aggtcgagcg ccgcatggcg ctgctgaagc
     1081 caacgctggc gatcgaggac ctgagagacg ccgacatcgt gatcgaggcg gtgttcgagg
     1141 acctcgccgt caagcaggac ctgttccgcc ggctcgaccg cgtaatgaag ccgggcgcga
     1201 tcctggccac caacacctcc acgctcgacg tcgatgcgat tgccgcggtc acgcggcggc
     1261 cgcaggacgt gctcggcacc catttcttca gccccgccaa cgtgatgcgg ctgctcgagg
     1321 tcgtgcgcgc caagcgcacg ggcaaggacg tgctcgcctc cgtgatgaag ctcgcgcgcc
     1381 ggctgaagaa ggtcgcggtc gtctccgggg tctgcgacgg cttcatcggc aaccgcatgc
     1441 tcgagcagta cgtccgccag tccttgttcc tggtcgacga gggcgccagc ccgcagaaga
     1501 tcgacgccgc gttgaccaag ttcggcatgg ccatgggacc gtttgcgatg tacgacatgg
     1561 ccggcatgga catcggttac gcgatccggc aacggcgcta caaggaaaag ccgcacatca
     1621 cctactcgcg catcggcgac cgcgtcgtcg aactgggccg gctcggccag aagaccggca
     1681 agggctggta ccgctacgag cccggcaacc gcgcgccgat tccggacccg gagatcgatg
     1741 ccctgataga tgcgtaccgc aaggagatcg gcatcaagcc gcgcgcgatc tccgacgaag
     1801 agatcgtgca gcgctgcatc ttcgcgctgg tcaacgaggg tgcgcgcatc ctcgacgagg
     1861 ggatcgcgca gcgtgccagc gacatcgacg ttgcgtatct cgcgggttac ggctttccgg
     1921 cggcgaccgg cggcccgatg ttccatgcgc agaccgtcgg cttcgacaag gtgatcaaga
     1981 caatggagcg gtttgccggc aacgcgcatg ccgaccccgc gttctggaac ccggcaccgc
     2041 ggctgcgccg ggcggcggtg gaagggcact ggtgacgcgg tcttgatcga accccggctg
     2101 ctgcggttcc tgctgtacga ctggctcgac gccgggtcac tgacccggcg cgcgcgctat
     2161 gccgagcact cgcgcgaaac cttcgacgcc gcactcgaag cggcggcacg gctcgccgcg
     2221 cgcgagttcg cgccgcataa ccgggcagcc gatttgaacg agccgcgcct cgttggcgag
     2281 caggtgcaac tgctccccga catcaagcgc gc
//
LOCUS       DBNHBY010000637         1589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1533794, whole genome shotgun sequence.
ACCESSION   DBNHBY010000637 DBNHBY010000000
VERSION     DBNHBY010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1533794"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="AC16HB_15335"
     CDS             <1..697
                     /locus_tag="AC16HB_15335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608211.1"
                     /translation="DTLWQKARGADAEAAAIAYRGVTLAREERELQLRLLQAQIEPHF
                     LFNTLANVRRLYRLHPEQGAQMMDSLRRYLRAALPSVRRADATLADELALVRSYLELL
                     RMRMADRLVYTIEAEAGLGATPFPPMIVVTLVENAIKHGLEPSEHGGRVDVRARRRGA
                     ILEVSVRDNGVGLNAQSTYGSGVGLANVRRQLIGRYGAAARLRLQPESPGLSATIEVP
                     LAADPAAAAFATP"
     gene            745..>1589
                     /locus_tag="AC16HB_15340"
     CDS             745..>1589
                     /locus_tag="AC16HB_15340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608212.1"
                     /translation="MRTTAVRPRPARLALLRTLQLAIAVAGTLVLISISSIFATIPDM
                     TASDRWQITIEVAAAKFADVAVQLAVLHFAVLPSPAPRALLALRFACMVLLGAVANTI
                     PMMLPADVPSIRLGLVESTTTMAWWTLSDALLIATFLVYQHGGQARSEEASRRLQQLQ
                     REQQAARRRLVEAQLQAMQARVDPQMFFDVLDSVQRLYSTDAPRAEALLDELIVFLRA
                     ALPQLRSPSSTLAQEFELAASYFRLQNLLGGAPAAVRIDLPDALAARAFPAGVILPLL
                     SEVRAA"
BASE COUNT          227 a          560 c          545 g          257 t
ORIGIN      
        1 ggacacgctg tggcaaaagg cgcgaggcgc cgacgccgag gccgcagcga tcgcctaccg
       61 tggcgtcacg ctcgcgcgcg aagaacgcga gttgcagctg cgcctgctgc aggcgcagat
      121 cgagccgcac ttcctgttca acacgctcgc caacgtccgc cgcctctacc ggctgcatcc
      181 cgagcagggc gcgcagatga tggacagcct caggcgttac ttgcgagcgg cgctgccgag
      241 cgtgcggcgc gccgatgcga cgctcgccga tgagctcgcg ctggtccggt cctaccttga
      301 actgctgcgc atgcggatgg ccgaccgcct cgtctacacg atcgaagccg aagccggcct
      361 cggcgccacg ccgttcccgc cgatgatcgt cgtcacgctc gtcgagaacg cgatcaagca
      421 cggactcgaa ccttcggagc acggcgggcg cgtcgacgtg cgcgcacggc ggcgcggcgc
      481 gatcctcgaa gtcagcgtgc gcgacaacgg cgtcggtctg aacgcgcagt cgacgtacgg
      541 gtcgggcgtc ggactggcca acgtgcgacg ccagctgatt ggtcgctacg gtgccgcggc
      601 gcgcctgcgt ctgcagccgg agtcgccggg gctgagcgcc acgatcgaag tcccccttgc
      661 tgcagatccg gccgccgcag cgttcgccac gccgtgaaca tgccaacgac cgccggggct
      721 aggcctgtga ctgccgaggg cgccgtgcga accacggcgg tgcggccacg gccagcgcgc
      781 ctggcgctct tgcggacttt gcagctcgcg atcgcggtcg ccggcacctt ggtgttgatc
      841 agcatcagca gcatcttcgc caccatcccg gacatgacgg cgagcgatcg atggcagata
      901 acgatcgagg tcgccgcggc gaagtttgcc gatgtcgcgg tgcagctcgc cgtgttgcac
      961 ttcgcggttc tgccatcgcc ggcgccgcgc gcactgctcg ccttgcgctt cgcgtgcatg
     1021 gttctgctcg gtgctgtcgc caatacgatt ccgatgatgc tgccggccga cgtcccgtcg
     1081 atccgcctcg ggctcgtgga gtcgacaacg acgatggcct ggtggacact gtccgacgcg
     1141 ctgctgatcg cgaccttcct cgtctaccag cacggcgggc aggccagaag cgaggaggcg
     1201 tcgcgtcggc tgcagcagct gcagcgcgaa cagcaggccg cgcggcggcg cctcgtcgag
     1261 gcgcaactgc aggcgatgca agcgcgcgtc gatccgcaga tgttctttga tgtcctcgac
     1321 agcgtgcagc gtctgtactc gaccgatgcg ccgcgggccg aggcgctgct cgacgagctc
     1381 atcgtgtttc tgcgtgccgc cctgccgcag ctgcgcagcc cctcctcgac gctggcgcag
     1441 gaattcgagc tcgcggcctc gtacttccgg ctccagaact tgctcggcgg cgcgccggcc
     1501 gcggtgcgta tcgatctgcc cgatgcgctg gcggcgcgcg cgttcccggc cggcgtaatc
     1561 ctgccgctgc tgtccgaggt acgggccgc
//
LOCUS       DBNHBY010000638         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1059412, whole genome shotgun sequence.
ACCESSION   DBNHBY010000638 DBNHBY010000000
VERSION     DBNHBY010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1059412"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="AC16HB_15345"
     CDS             <1..799
                     /locus_tag="AC16HB_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5608213.1"
                     /translation="DEETGERFELRTRCVFNAAGVWVDPIRQLADPSARPVTTVSQGS
                     HIVVDREFLPGSAGLMIPRTRDGRVLFAIPWHHKLLIGTTDQPRADAPFDPQPSLQEI
                     DFIIETAAGYLARPIAHADVRAAFAGLRPLFSPKAAGSTAKISREHAVLTEFGCVISV
                     VGGKWTTYRRMAVDALDAAARAGVLPAGQSRTADLMLMQDAELVAAADADPGSPRALQ
                     NFAAHCRRFTQARTNTDVLARRARLDFLDADASGRVAARLRSSAAAD"
     gene            complement(860..1960)
                     /locus_tag="AC16HB_15350"
     CDS             complement(860..1960)
                     /locus_tag="AC16HB_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR5608214.1"
                     /translation="MKKTLVALAIAGAFSGAAFAQSSVTLYGIADVGFQITDFSRSGV
                     SNVTGVDSGIRNGSRWGLRGSEDLGGGMNAIFQLENGYQVDTGTINQGGRMFGRQAYV
                     GLQGAFGTFALGRMATLGSGTGAFDTWSDIDPFYTGYGLAGTQNVFSEAGSLRIDNAF
                     MYRTPNISGFQAGWQHSFNFNGAEAAGNGNNLRVDSLAVSFGAGPFYGAINYVIGKFP
                     NVTTGTRPSDEKLLKIGATYDLKVVKLHGGWGDEKNVRNALISAVGGTADGTDAKAWF
                     IGASVPFGPNNSKFMAAFGKRDGKSQTIGTTAFNADRKYYGLGYEYFMSRRTIIHFHA
                     AKGDGSNTLAEGTAATDFANKKEYALGVTHFF"
     gene            complement(2241..>2711)
                     /locus_tag="AC16HB_15355"
     CDS             complement(2241..>2711)
                     /locus_tag="AC16HB_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR5608215.1"
                     /translation="YLNAKNDNPVLRPQDLQGLLISTGGNLTAINGILGGLFINAFDV
                     DSLRGLVGPTDMKVYHLGLQWNVGPGQLLASYTNSKDKARSPWATADATVDLFGLAYF
                     YNLSKRSALYASGAYAVNKDQSRAALGAACCRGGWTTAPGEDSRVLQAGIRHTF"
BASE COUNT          468 a          914 c          851 g          478 t
ORIGIN      
        1 ggacgaagag accggcgaac ggttcgaact gcgcacgcgc tgcgtattca acgccgccgg
       61 cgtgtgggtc gacccgatcc gccagctcgc ggacccgtcc gcccgaccgg tcacgacggt
      121 gagccagggc tcgcacatcg tcgtcgaccg cgagtttctt cccggcagcg ccggcctgat
      181 gattccgcgc acgcgcgacg gccgcgtgct gttcgcgatt ccatggcatc acaagctgct
      241 gatcggcacc accgatcagc cgcgggccga cgcgccgttc gatccgcagc catcgctgca
      301 ggagatcgat ttcatcatcg agaccgccgc cggctatctc gcgcgtccga tcgcgcatgc
      361 cgacgtgcgc gcggcgttcg cggggctgcg gccgctgttc tcgcccaagg ccgccggcag
      421 caccgcgaag atctcgcgcg aacacgcggt gctgaccgag ttcggctgcg tgatctcggt
      481 ggtcggcggc aagtggacga cttatcgccg catggcggtc gatgcgctcg acgcggcggc
      541 ccgcgctgga gtgctgcccg cggggcagtc gcggacggcc gatctgatgc tgatgcagga
      601 tgcggaactg gtcgccgcgg ccgacgccga tcccggatcg cctcgcgcgc tgcagaactt
      661 cgccgcgcac tgccggcgct tcacgcaggc gcgcacgaat accgacgtgc tggcgcggcg
      721 cgcccggctc gattttctcg acgccgatgc ctcggggcgg gtcgcggccc gcctgcggag
      781 ttcggcagcg gcggactgat agaggcgaaa aaaaggccgc ccgcaggcgg ccttggattc
      841 cctgaacttg ctgcgcttct tagaagaaat gcgtgacgcc gagtgcgtac tctttcttgt
      901 tcgcgaagtc cgtcgccgcc gtgccttccg ccagcgtgtt cgagccatcg cccttcgccg
      961 catggaagtg aatgatcgta cgacgcgaca tgaagtactc gtagccgagg ccgtagtact
     1021 tgcgatccgc attgaacgcc gtcgtgccga tggtctgcga cttgccgtcg cgcttgccga
     1081 acgcggccat gaacttgctg ttgttcgggc cgaacgggac cgacgcgccg atgaaccacg
     1141 ccttggcatc ggtgccatcc gccgtgccgc cgaccgccga gatcagcgcg ttgcggacgt
     1201 tcttctcgtc accccagccg ccgtgcagct tcacgacctt cagatcgtac gttgcgccga
     1261 tcttcagcag cttctcgtcc gaaggcctcg ttcccgtcgt cacattcggg aatttgccga
     1321 tcacgtagtt gatcgcgccg tagaacggac cggcgccgaa cgacaccgcc aagctgtcga
     1381 cgcgcagatt gttgccgtta ccggcggcct cagccccgtt gaagttgaac gagtgctgcc
     1441 agcccgcctg gaagccgctg atgttcggcg tgcgatacat gaacgcgttg tcgatgcgca
     1501 gcgagccggc ttccgagaag acgttctggg tcccggccag gccgtaaccc gtatagaacg
     1561 ggtcgatgtc gctccacgtg tcgaacgcgc cggtgcccga accgagcgtc gccatgcggc
     1621 cgagggcaaa cgtaccgaat gcgccctgca gtccgacgta ggcctgacga ccgaacatgc
     1681 ggccgccctg attgatggtg cccgtatcga cctgatagcc attctcgagc tggaagatcg
     1741 cgttcatgcc accgcccaga tcctccgatc cacggagacc ccagcggctg ccgttgcgga
     1801 tgccggagtc gacgcccgtc acgttcgaca cgccgctgcg gctgaagtcg gtaatttgaa
     1861 agccgacgtc agcgatgccg tacagcgtga ccgatgactg cgcgaacgca gccccgctga
     1921 acgcgcccgc gatcgccaga gccactaatg tcttcttcat ctgtatctcc agtttgtcaa
     1981 tggaacaaca ccaagccatc caccccggac acgctgcgtg tttcgcaacc gcatgcgcct
     2041 tgatgtctcg cactcccaga ggaagcttgc cccaattttt tcagacttcc gaggcaccgg
     2101 caaaagtgga ctcactcaaa cacccaattt aggtgccgtc ctgtcggatt cctgcaacag
     2161 gtccggcgag cgaaccgggg cgaagaacaa aaaggggtgc ggaagcaccc ctttgccggc
     2221 gacccggcgg ggaacgcagg tcagaaggta tggcgtatgc ccgcctgaag aaccctgctg
     2281 tcttcgcccg gtgccgtcgt ccagccgccg cggcagcagg ctgcgccgag tgcggcccgc
     2341 gactgatcct tgttgaccgc gtatgccccc gatgcgtaca gcgccgaacg cttggacagg
     2401 ttgtagaaat acgcgagacc gaacaggtcg accgtcgcat cggccgtcgc ccacgggctg
     2461 cgcgccttgt ccttcgaatt cgtgtacgaa gcgagcaact ggcccgggcc cacgttccac
     2521 tgcaggccga ggtggtagac cttcatgtcg gtcggaccca ccagcccgcg cagcgaatcg
     2581 acatcgaacg cgttgatgaa caggccaccg aggatcccgt tgatggccgt gaggttgccg
     2641 ccggtgctga tcagcagccc ctgcaggtcc tgcggacgca gcaccggatt gtcgttcttg
     2701 gcattcaggt a
//
LOCUS       DBNHBY010000639         3369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1803712, whole genome shotgun sequence.
ACCESSION   DBNHBY010000639 DBNHBY010000000
VERSION     DBNHBY010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1803712"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16HB_15360"
     CDS             complement(<1..608)
                     /locus_tag="AC16HB_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5608216.1"
                     /translation="MPTSPRVYIIDDDSGVRDSLALWLGMRGHRTQVFESAEAFLAGA
                     HAPLRGCAVVDLKLGGIDGLELQRQLAARAIALPLIFVTGHGDVATARNALKAGAFDF
                     IEKPIDNDRLVELVGAALAEDGRRAAHEAEAQDLAERMKRLTQRERQVLAQVVSGKHN
                     REIAADLGISARTVEVYKARVMDKLQVRRVPDLVRLVLNAEG"
     gene            837..>3369
                     /locus_tag="AC16HB_15365"
     CDS             837..>3369
                     /locus_tag="AC16HB_15365"
                     /inference="COORDINATES: protein motif:HMM:NF009288.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HIR5608217.1"
                     /translation="MTFDWLLPLLALLPFAGGLVLLATPNAQRRRAAAIAGIVTLTGC
                     AIVLALSPAVFADDIARWHVDWLPQLGLAFGFRLDGLAWLFALLVCGIGALIVLYAAY
                     YLHHDDPAARFFAFLLFFMGAMLGVVLADNLLLLVVYWELTSLSSFLLIGYWNHRGDA
                     RAGARMALAITGAGGLALLAGVLLLGHIVGGYGLDLVLASGNVIRAHPLYLPALILIL
                     LGAFTKSAQFPFHFWLPHAMAAPTPVSAYLHSATMVKAGVFLLARLYPALGGSEAWFW
                     IVAPVGLATLVLGAYIAIFQHDLKGLLAYSTISHLGLITLLFGIDTRLAVVAGVFHIL
                     NHATFKASLFMAAGIIDHESGSRDMRKLNGLWKYMPVTGMLGTVAAAAMAGVPLANGF
                     LSKEMFFTEALAKEVPAAIDWILPAGAVIAGIFSVAYSTRFIHDVFFNGEPVGLTKTP
                     HEPPHFMRVPVEILVVVCLAVGLAPSLTIAPLLAVGAQAALFGGPFAPLPADQLSIWH
                     GFNLPLAMSALALAGGFALYFGLQRTVNLHRLVRLPLWIAGGGREAFLRFTDTKLRIA
                     RAVTATIQNGNLQRYLMLLVLLAILAGFAPFIEGVPASPPARFATPANAAVVAVWAVG
                     VLATLGAARAYRQRLIALVLLGAVGLVVSLAFAYLSAPDLALTQLLVEMVTIILMMLA
                     LRWLPAESAPEPGRARKLRDALLALVGGAGIAALAYALITQPFDSIAPYFLQNSIAQG
                     GGANAVNVIIVDFRGYDTLGEITVLGIAGLVIHALLAGFAPKPGAAPPARSLLLALAA
                     RLLLPLAALLAIHLYVRGHNAPGGGFSAGLVLALALMLQFIALGGE"
BASE COUNT          422 a         1195 c         1134 g          618 t
ORIGIN      
        1 tcgccctcgg cgttcagcac cagccgcacg aggtccggca cgcgcctcac ctgcagcttg
       61 tccatcacgc gtgccttgta cacctcgacg gtgcgcgcgc tgatgccgag atcggcagcg
      121 atctcgcggt tgtgcttgcc ggagacgacc tgcgccagca cctggcgttc gcgctgcgtg
      181 aggcgcttca ttcgctccgc caggtcctgc gcctcggcct cgtgcgcggc gcgccgccca
      241 tcctcggcaa gcgcggcgcc gaccagctcg accagccgat cgttgtcgat cggcttctcg
      301 atgaaatcga acgcgcccgc cttcagcgcg ttgcgcgcgg tggcgacgtc accgtgtccg
      361 gtcacgaaga tcagcggcag cgcaatcgct cgcgcggcga gctgccgctg cagttcgagc
      421 ccgtcgatgc cgcccagctt caggtcgacg accgcacagc cgcgcagcgg cgcgtgcgcg
      481 ccggcgagga acgcctcggc cgactcgaag acctgggtcc gatgaccgcg catgccgagc
      541 cacaacgcga gcgaatcgcg cacgccggaa tcgtcgtcga tgatgtaaac gcggggcgaa
      601 gtcggcatcg gttggcaggc attcagcgcg gaggtcttct tacgaattat cgcgtaggcg
      661 gctcctcgga tcacctgttt cgacgcgagt tcctgcggcg agtacgggta aacgccgggc
      721 cggagtgctc gcgtcccgct cctatactgc ggcgccggtc gaagcaggct gctacgtagt
      781 ttccgcagcc gccggcaatc gcaaccgcac tcaccgcggc cgaccccgtc tcactgatga
      841 cattcgactg gctgctgccg ctgctggcac tgctgccgtt tgccggcggg ctggtgctgc
      901 tcgccacgcc gaacgcgcag cgacgccgcg cggcggcgat cgccggcatc gtcacgctga
      961 cggggtgcgc gatcgtgctg gcgctgtcac ccgcggtgtt cgccgacgac atcgcgcgct
     1021 ggcatgtcga ctggctgccc cagctcggcc tggcgttcgg cttccggctc gacggcctgg
     1081 cctggctgtt tgcgctgctc gtctgcggca tcggggcgct gatcgtgctg tatgccgcct
     1141 actacctgca tcacgacgat ccggcggcgc gcttcttcgc cttcctgctc ttcttcatgg
     1201 gcgcgatgct cggcgtcgtg ctcgcggaca acctgctgct gctcgtcgtc tactgggaac
     1261 tgacgagcct gtcgagcttc ctgctgatcg gctactggaa ccaccgtggc gacgcgcgcg
     1321 ccggcgcccg catggcgctc gcgatcaccg gcgctggcgg gctcgcgctg ctggcgggcg
     1381 tgctgctgct cggccacatc gtcggcggct acggactcga tctcgtgctc gcgtcgggca
     1441 acgtgatccg cgcgcatccg ctgtacctgc cggcgctgat actgatcctg ctcggcgcgt
     1501 tcaccaagag cgcgcagttc ccgttccact tctggctgcc gcacgcgatg gcggcgccca
     1561 cgcccgtatc ggcgtacctg cactcggcga cgatggtgaa ggccggcgtg ttcctgctcg
     1621 cgcggctgta tccggcgctc ggcggttccg aagcgtggtt ctggatcgtc gcgcccgtgg
     1681 gcctggccac gctggtgctc ggcgcctaca tcgcgatctt ccagcatgac ctgaagggtc
     1741 tgctcgccta ttcgacgatc agtcacctcg gtctgatcac gctgctgttc ggcatcgaca
     1801 cgcggctcgc cgtggtggcc ggcgtgttcc acatcctcaa ccacgccacg ttcaaggcgt
     1861 cgctgttcat ggcggcgggc atcatcgacc acgaatcggg ttcgcgcgac atgcgcaagc
     1921 tcaatggcct gtggaagtac atgccggtga ccgggatgct cggcaccgtg gcggcggccg
     1981 cgatggcggg ggtgccgctc gccaacggat tcctgtcgaa ggaaatgttc ttcaccgagg
     2041 cgctggcgaa agaagtgccc gccgcgatcg actggatcct gccggccggc gcggtgatcg
     2101 cgggcatctt cagcgtcgcg tactcgacgc ggttcatcca cgacgtgttc ttcaacggcg
     2161 agccggtcgg gctgaccaag acgccgcacg aaccaccgca tttcatgcgc gtgccggtcg
     2221 agatcctggt ggtggtgtgc ctggcagtgg ggctggcgcc gtcgctgacg atcgcgccgt
     2281 tgctggcggt cggcgcccag gcggcgctgt tcggcggacc attcgcgccg ctgccggcgg
     2341 accagctttc gatctggcat gggttcaacc tgccgctcgc gatgagcgcg ctcgcgctcg
     2401 ccggcggctt tgccttgtac ttcgggctgc agcgcacggt caacctgcat cgcctggtgc
     2461 gcttgccgct gtggatcgcc ggcggcggcc gcgaggcgtt cctgcgcttc accgacacca
     2521 agctgcgcat cgcgcgggcg gtcacggcca cgattcagaa cggcaacctg cagcgctatc
     2581 tgatgctgct ggtgctgctt gcaatcctcg ccggcttcgc gccgttcatc gaaggcgtgc
     2641 ccgcctcacc gcccgcgcgg ttcgccacgc cggccaatgc cgccgtcgtc gccgtgtggg
     2701 cggtcggcgt gctcgctacg ctcggcgccg cgcgcgccta ccggcaacgg ttgatcgcgc
     2761 tggtgctgct gggcgcggtc gggctcgtcg tcagcctcgc tttcgcctac ctgtcagcgc
     2821 ccgacctcgc gctgacgcaa ctgctggtcg agatggtgac gatcatcctg atgatgctcg
     2881 cgctgcgctg gctgccggcc gaaagcgcgc ccgagcccgg ccgcgcgcgc aagctgcgcg
     2941 atgcgctgct cgcacttgtc ggcggcgccg gcatcgcagc cctggcgtac gcgctgatca
     3001 cgcaaccgtt cgattcgatc gcgccgtact tcctgcagaa ctcgatcgca cagggtggcg
     3061 gcgccaacgc ggtcaacgtg atcatcgtcg atttccgcgg ctacgacacg ctgggcgaga
     3121 tcacggtgct cggcatcgcc ggcctcgtga tccacgcgct gctcgccggg ttcgcgccga
     3181 agccgggggc tgcgccgccg gcgcgctcgc tgctgctcgc gctcgccgcg cggctgctgc
     3241 tgccgctcgc cgcgctgctc gcgatccatc tgtacgtgcg cggacacaac gcgcccggcg
     3301 gcggcttcag cgccggtctg gtgctggcgc tcgcgctgat gctgcagttc attgcgctcg
     3361 gtggagagt
//
LOCUS       DBNHBY010000640         4942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_992917, whole genome shotgun sequence.
ACCESSION   DBNHBY010000640 DBNHBY010000000
VERSION     DBNHBY010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_992917"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /locus_tag="AC16HB_15370"
     CDS             <1..1763
                     /locus_tag="AC16HB_15370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5608218.1"
                     /translation="QSATSQVLSAISRSPTELTPVLDTIVRTASRLVGADHAGIMQLR
                     DGRFVLAAAHGAEPEFVDFLRDHPPAVDLGTLCGRTVITRRTVYIEDTRTDPRYEWRE
                     AAERGNYRTALGAPLLRQGEPIGVLWVAHRTVRAFTAEQVALITTFADQGVIAIENVR
                     LFSELQSRTTQLTRTVEQLQALAEVGQAVSSTLDLETVLATIVSRATELTGMDGGVIY
                     EYDESQQSFVPRATDRLPDELTQALRDAPMYKGEGAVGRVALHQAPVVIGDIADARLY
                     QSRVRQMLLRHGYRSLLAVPLLREDRLLGGLVVHRRRAGEFEAPIIELLKAFATQSAL
                     AIQNARLFREIEEKSRQLEIASRHKSAFLANMSHELRTPLNAIIGFARIVIRRVQGQV
                     EAKQYENLEKILASAQHLLALINAILDIAKVEAGRVEVRAGEVALAAVLEQCMRTVEP
                     LLHDGVTLETSFDPSVPPIVADEEKLRQIVINLLSNAAKFTASGRIRVAAQVAGGRVE
                     IAVADTGIGIPADKLESIFEEFEQVDASSTRAHSGTGLGLAIARRLARLLGGDIRVES
                     VLGAGATFTLTLPLRYRPAE"
     gene            1763..2125
                     /locus_tag="AC16HB_15375"
     CDS             1763..2125
                     /locus_tag="AC16HB_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608219.1"
                     /translation="MKTILIVEDLALNRDLLAQLLGDNFRLLFAEDGVAALERAAAAR
                     PDLILMDLSLPRMDGWESTRRLKADPELALIPVIALTAHAMTDDEVRARQCGCDDFLT
                     KPIDEDMLFDKLAHYLGH"
     gene            2115..3491
                     /locus_tag="AC16HB_15380"
     CDS             2115..3491
                     /locus_tag="AC16HB_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5608220.1"
                     /translation="MGTEPATVLIVDDEPLNLDLLAQELDAAGYRTVAAASGAEALAL
                     ATSRAPDLILLDVMMNGIDGYETCRRLKANDATRAIPVIFLTALKETFEKVRAFRLGA
                     VDYVTKPFESEELLARVGTHIALRREIEAHRTSKAAIRVLVEDQHSDAMIGESPALAR
                     VRAAIAQVGPTDSTVLIQGETGTGKELVARAIHAASARRDRPLIKLNCAALPRELVES
                     ELFGHEKGAFTGAIQQRRGRFELADGGTLFLDEVGELPLEAQAKLLRVLQDGEFERVG
                     GTRGLRVDVRVIAATNRDLHAEVGAGRFRADLYYRLNVFPIALPPLRERREDVPPLLR
                     HYAARSARRLGRALPEFAPAFIERARAYDWPGNIRELQNVVERALIMSTGGPLDTSEP
                     LGPSAARQRDPDVAGGTIEDVERSHIRNVLETTQWRIEGSAGAAERLGLNPSTLRGRM
                     RKLGIRKP"
     gene            3641..4057
                     /locus_tag="AC16HB_15385"
     CDS             3641..4057
                     /locus_tag="AC16HB_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608221.1"
                     /translation="MPSGLLRSRAKRKDSAMSRRLCKNTRISSLLVAALTTAALGWSA
                     ANAQTPDQALAPERMLAQLERDFWACDYASTVMLLDSGTAMACSEISEKFKRLMFKGD
                     FPALHAYWLQNKAARYRALAAGWQDAFDGRVAAAER"
     gene            complement(4058..>4942)
                     /locus_tag="AC16HB_15390"
     CDS             complement(4058..>4942)
                     /locus_tag="AC16HB_15390"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5608222.1"
                     /translation="ERELRIEAASGASAGAVNAALLAHGLAQDGTAGARTALRAFWQR
                     LSDQAITAPDYALKALLTMTRWLAPPQWNPLDVNPLRTLLADAIDFERVRSARRIGLF
                     IAATRVDTGTLRVFHENELTLEVLLASACLPLLHHTIEIDGVAYWDGGLAANPPLYSL
                     IDHCTADDLVLVTMHPSRRDSTPRNADAIHARLAEISFTAAFFAELQGVLSAQRAARR
                     QWFPIGRATRRLRRLRVHVIDGGDLTALLPAASRMDVRRELVEELFAHGRTRAQQWID
                     RAAAPGAPVDSVEALLFR"
BASE COUNT          804 a         1702 c         1717 g          719 t
ORIGIN      
        1 gccagagcgc caccagccag gtgctgagcg cgatcagtcg ctctcccacc gagctgacgc
       61 cggtgctcga caccatcgtc aggacggcga gccggctggt cggcgccgac catgcgggga
      121 tcatgcagct gcgcgatggc cggttcgtgc tcgccgccgc ccatggcgcc gagccggagt
      181 tcgtcgactt cctgcgcgac catccgcccg cggtcgacct cggcacgctc tgcggccgca
      241 cggtgatcac ccgacgcacc gtctacatcg aagacacgcg aaccgacccg cgctacgaat
      301 ggcgcgaggc agccgagcgc ggcaactacc gcaccgcgct cggggccccg ctgctgcggc
      361 agggcgagcc gatcggcgtc ctgtgggtcg cgcacaggac ggtacgtgcc ttcacggccg
      421 aacaggttgc cctgatcacg acgttcgcgg accagggcgt gatcgcgatc gagaacgtgc
      481 ggctgttcag cgaactgcag tcgcgcacga cgcagctgac gcgcacggtg gagcaactgc
      541 aggcgctggc ggaggtcggt caggccgtca gctcgacgct cgacctcgaa acggtgctgg
      601 ccacgatcgt gtcgcgcgcc accgaactca cgggcatgga cggcggcgtg atctacgaat
      661 acgacgagtc gcagcagtca ttcgtgccgc gtgcgaccga tcgcctgccc gacgaactca
      721 cgcaggcgct gcgcgatgcg ccgatgtaca agggcgaggg cgccgtgggc cgggtggcgc
      781 ttcaccaagc gcccgtggtg atcggcgaca tcgccgatgc ccggctgtac cagagccggg
      841 tgcgccagat gctgctgcgg cacggctacc gctcgctgct ggcggtgcca ctgctgcgcg
      901 aagaccgcct gctcggcggg ctggtcgtgc accggcgccg cgccggcgag ttcgaagcac
      961 ccatcatcga gctgctgaag gcattcgcca cccagtccgc gctggcgatc cagaacgcgc
     1021 gcctgttccg cgagatcgag gaaaagagcc gccaactcga gatcgcgagc cggcacaaga
     1081 gcgcgttcct cgccaacatg tcgcacgagc tgcgcacgcc gctgaacgcc atcatcggct
     1141 tcgcgcgcat cgtgatacgc cgcgtgcagg gacaggtcga ggcgaagcag tacgagaacc
     1201 tcgagaagat cctggcgagc gcgcagcacc tgctcgcgct gatcaatgcg atcctcgata
     1261 tcgccaaggt cgaggccggg cgcgtcgagg tgcgcgcggg cgaggtcgcg cttgccgcgg
     1321 ttctcgagca gtgcatgcgc accgtcgagc cgctgctgca cgatggcgtg acgctggaga
     1381 cgagcttcga cccgagcgtg ccgccgatcg tggccgacga ggagaagctg cggcagatcg
     1441 tcatcaacct gctcagcaac gccgccaagt tcaccgcgag cggccgcatc cgcgtcgcgg
     1501 cgcaggtggc gggcggccgg gtcgagatcg cggtcgcgga caccggcatc ggcattccgg
     1561 ccgacaagct cgagtcgatc ttcgaggagt tcgagcaggt cgatgccagc agcacgcgcg
     1621 cgcacagcgg caccggcctg ggtctggcga ttgcgcgccg gctcgcgcgg ctgctcggcg
     1681 gcgacatccg cgttgaaagc gtgctcggcg cgggcgcgac gttcacgctg acgctgccgc
     1741 ttcgttaccg gcccgccgag tgatgaagac gatcctgatc gtcgaggacc tcgcgttgaa
     1801 ccgcgatctg ctggcgcagc tgctcggcga caatttccgg ctgctgtttg ccgaggacgg
     1861 cgtagccgcg ctcgaacgcg ccgcggccgc gcgacccgac ctgatcctga tggacctgtc
     1921 gctgccgcgc atggacgggt gggaatccac gcgccggctg aaggccgacc cggagctcgc
     1981 gctcattccc gtcatcgcgc tcaccgcgca tgcgatgacg gacgacgagg tgcgtgcgcg
     2041 ccaatgcggt tgcgacgatt tcctgaccaa gccgatcgac gaagacatgc tgttcgacaa
     2101 actcgcgcac taccttgggc actgaacccg ccaccgtcct gatcgtcgac gacgaaccgc
     2161 tgaacctcga cctgctggcg caggagctcg acgcagccgg ctatcgcacc gtcgctgcgg
     2221 caagcggcgc agaagccctg gcgctggcaa cgagccgcgc gcctgacctg atcctgctcg
     2281 acgtgatgat gaacggcatt gacggttatg aaacctgccg gcggctgaag gcgaacgatg
     2341 cgacgcgcgc gattccggtg atcttcctga ccgcgctgaa ggagaccttc gaaaaggtgc
     2401 gcgcgttccg gctcggcgcg gtcgactatg tgaccaagcc gttcgagtcg gaagaactgc
     2461 tggcgcgcgt cggcacgcac atcgccttgc ggcgcgagat cgaggcgcac cgcacgtcga
     2521 aggcggcgat ccgcgtgctg gtcgaagacc agcacagcga cgcgatgatc ggcgaatcgc
     2581 cggcgctggc gcgcgtgcgc gcggcgatcg cacaggtggg gccgaccgac agcaccgtgc
     2641 tgatccaggg cgagaccggc accggcaagg aactggtcgc gcgcgcgatc catgccgcga
     2701 gcgcgcgccg cgaccggccg ctgatcaagc tcaactgcgc ggcgctgccg cgggaactgg
     2761 tggaaagcga actgttcggc cacgagaaag gcgcgttcac cggcgcgatc cagcagcgtc
     2821 gcggccgctt cgaactcgcg gacggcggca cgctgttcct cgacgaagtg ggcgaactgc
     2881 cgctcgaagc gcaggcaaag ctcctgcgcg tgctgcaaga cggcgagttc gagcgcgtcg
     2941 gcggcacgcg cggcctgcgg gtcgatgtgc gcgtgatcgc ggcgaccaac cgcgatttgc
     3001 acgcggaggt tggggcgggg cgttttcgcg ccgatctgta ttaccggttg aacgtgtttc
     3061 cgatcgcgct gccgccgttg cgcgagcgcc gcgaagacgt tccgcctctg ctgcgccact
     3121 acgcggcgcg cagcgcgcgc cgcctcggac gcgcgctgcc ggagttcgcc ccggcattca
     3181 tcgagcgcgc gcgcgcctac gactggccgg ggaacatccg cgaactgcag aacgtcgtcg
     3241 agcgcgcgct gatcatgtcc acgggtggcc cgctcgacac cagcgagccg ctcggtccat
     3301 cggcggcgcg gcagcgcgac ccggacgtcg cgggcgggac gatcgaagac gtcgagcgct
     3361 cacacatccg caacgtcctc gaaacgacgc agtggaggat cgagggcagc gccggcgcgg
     3421 ccgagcggct cgggttgaat ccgagcacgc tgcgcggacg catgcgcaag ctcggcattc
     3481 gcaagcctta gccgccgtcc catgcgacgg cgcacgccgc cgcatttggc agccgtcgcc
     3541 cgctcatcgc cgtgccttcg gattgtccct cccgcaagtg cttgaaattg cgtcacattt
     3601 cacgaccggc cgcgctggca cgccacgtgc gaagcaaagc gtgccgagcg ggctccttcg
     3661 atcccgggca aaacgaaagg acagtgccat gtcacgccga ctctgcaaga acacgcggat
     3721 ctcatcgctg ctcgttgctg ccttgaccac tgccgccctg ggttggagtg cggccaatgc
     3781 gcagacgccg gatcaggcgt tggcaccgga gcgcatgctc gcgcaactcg agcgcgattt
     3841 ctgggcttgt gactacgcct cgaccgtgat gcttctggac agcggaaccg cgatggcctg
     3901 tagcgagatc agcgagaagt tcaagcggct catgttcaag ggcgactttc cggcgctgca
     3961 cgcctactgg cttcagaaca aggccgcgcg ctatcgcgcg ctcgccgccg ggtggcagga
     4021 cgctttcgac ggccgcgtcg ccgctgcgga gcgctgatca gcgaaacagc agcgcctcga
     4081 cgctgtcgac cggcgcgccg ggcgccgccg cgcgatcgat ccactgctgc gcacgcgtgc
     4141 ggccgtgcgc gaacagttcc tcgaccagtt cgcgccgcac gtccatgcgg ctagcggccg
     4201 gaagcagcgc ggtcaggtcg ccgccgtcga tcacgtgcac gcgcagccgg cgcaggcggc
     4261 gcgtcgcacg gccgatcggg aaccattgcc ggcgtgcggc gcgctgcgcc gacagcacgc
     4321 cctgcagttc ggcgaagaac gcggccgtga agctgatctc ggccagccgc gcatgaatgg
     4381 cgtcggcgtt gcgcggcgtg ctgtcccttc gggacgggtg catcgtcacg agcacgaggt
     4441 cgtcggccgt gcagtgatcg atcagcgaat acagcggcgg gttcgccgcc agcccgccgt
     4501 cccagtacgc gacgccgtcg atctcgatcg tgtgatgcaa cagcggcagg cacgcggagg
     4561 cgagcagtac ctcgagcgtc agctcgtttt catggaacac gcgcagtgtg ccggtatcga
     4621 cgcgcgtggc ggcgatgaac agcccgatcc ggcgcgcgct gcgcacgcgc tcgaaatcga
     4681 tcgcgtcggc cagcagcgtg cgcagcggat tgacgtcgag cggattccac tgcggcggtg
     4741 ccagccagcg cgtcatcgtg agcagcgcct tcagcgcgta gtcgggtgca gtgatggcct
     4801 ggtccgacag gcgctgccag aaggcgcgca gcgccgtccg cgcgccggcc gtgccgtcct
     4861 gcgccagccc gtgcgcgagc agcgcggcgt tgaccgcacc cgcgctcgcg ccgctcgccg
     4921 cttcgatccg cagttcgcgc tc
//
LOCUS       DBNHBY010000641         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_112459, whole genome shotgun sequence.
ACCESSION   DBNHBY010000641 DBNHBY010000000
VERSION     DBNHBY010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_112459"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="AC16HB_15395"
     CDS             <1..174
                     /locus_tag="AC16HB_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HIR5608223.1"
                     /translation="MPKGVPVATFAIGESGAANAALFAVAMLAAQDNELAMKLAQYRA
                     TQTQAAKNMALPL"
     gene            174..1346
                     /locus_tag="AC16HB_15400"
     CDS             174..1346
                     /locus_tag="AC16HB_15400"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR5608224.1"
                     /translation="MSAAEPMNTVSPGDWLGLLGGGQLGRMFCMAAQSMGFRVCVLDP
                     GDDSPAGAVADDHIRADYLDDSALIEIAQRCRAVTTEFENVPAAAMRRLADHCVVRPA
                     ADSVAVAQDRIHEKRFMVNHGIAVGPHVVVTRIDDLEAADLKLFPAILKTARLGYDGK
                     GQVSAEKREDAIEAWSRGGRVPCVLEQRLHLHQELSCLVCRGADGAAVTFPVVENAHR
                     GGILAASIAPARVPGDTADKAREVAQKVAEHLNYVGVLCVEFFVLEDGRLLVNEIAPR
                     PHNSGHFTIDACVTSQFEQQARILAGLPLGSTLLLAPAVMLNILGDVWFHDGQKREPA
                     WADVLRVDGAKLHLYGKREARPCHKMGHVTVLADSVAQALERATEVARVLGIDPPK"
     gene            1343..>1947
                     /locus_tag="AC16HB_15405"
     CDS             1343..>1947
                     /locus_tag="AC16HB_15405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5608225.1"
                     /translation="MSAATNDVPPVDAAAIERAAALLARGRLVAFPTETVYGLGADAD
                     DRAAVAAIFAAKGRPADHPVIVHVQDARALDTWAREVPAAAHRLARVFWPGPLTLVLK
                     RGARAHESLTGGQDTVGLRCPAHPWARALLAAFCAARGDPAAALAAPSANSFGRISPS
                     RAEHVRADLGERPTGKVDLILDGGACPIGIESTIVDLSGAPP"
BASE COUNT          280 a          672 c          709 g          286 t
ORIGIN      
        1 atgcccaagg gcgttcccgt ggcgacgttt gcgatcggcg aaagcggcgc cgccaacgcg
       61 gcgctgttcg cggtggcgat gctcgctgcg caggacaacg agctggctat gaagctcgcg
      121 cagtaccgcg ccacgcagac ccaggcggcg aagaatatgg cgctgccgtt gtgatgtcgg
      181 ctgctgagcc tatgaacacc gtgtcccccg gcgattggct cggcctgctc ggcggcgggc
      241 agctcggacg catgttctgc atggcagcgc agagcatggg atttcgcgta tgcgtgctcg
      301 atccgggcga cgacagcccg gccggcgcgg tggcggacga tcacatccgc gccgattacc
      361 tcgacgattc cgcgctgatc gagatcgcgc agcgttgccg tgcggtgacg accgagttcg
      421 agaacgtgcc ggccgcggcc atgcgccggc tcgccgatca ctgcgtggtg cggcccgcgg
      481 ccgacagcgt ggcggtcgcg caggaccgca tccacgagaa gcgcttcatg gtcaaccacg
      541 gcatcgccgt cggtccgcac gtcgtggtga cgcggatcga cgatctcgag gccgcggacc
      601 tgaagctctt tcctgcgatc ctgaagaccg cgcggctcgg ctacgacggc aagggccagg
      661 tcagcgcgga gaagcgcgag gacgcgatcg aagcctggtc gcgcggcggc cgcgtgccgt
      721 gcgtgctcga gcagcggctg cacctgcacc aggagttgtc gtgcctcgtc tgtcgcggcg
      781 ccgacggcgc ggcggtgacg ttcccggtcg tcgagaacgc gcaccgcggc ggcattctcg
      841 cggcatcgat cgcgccggcg cgcgtgccgg gcgacaccgc ggacaaggcg cgcgaagtcg
      901 cgcagaaagt cgccgagcat ctgaactacg tcggcgtgct gtgcgtcgag ttcttcgtgc
      961 tcgaggacgg gcggctgctc gtcaacgaga tcgcgccgcg cccgcacaac tcgggtcact
     1021 tcacgatcga cgcctgcgtg acgagccagt tcgagcagca ggcgcgcatc ctcgccgggt
     1081 tgccactggg cagcactttg ctgctcgcgc ccgcggtgat gctgaatatt ctcggtgatg
     1141 tctggttcca cgacgggcag aagcgcgagc cggcgtgggc cgacgtgctg cgcgtcgacg
     1201 gcgccaagct gcatctgtac ggcaagcgcg aggcgcggcc ctgccacaag atggggcacg
     1261 tgaccgtgct cgccgactcg gtggcgcagg cgctcgagcg cgcgaccgag gtggcgcgcg
     1321 tgctcggcat cgatccaccg aaatgagcgc cgcgacgaac gatgtgccgc cggtcgatgc
     1381 ggcggccatc gagcgcgcgg ccgcgctgct cgcgcgcggc aggttggtcg catttccgac
     1441 cgagaccgtc tatggcctcg gcgccgatgc cgacgatcgc gccgccgtgg ctgcgatctt
     1501 cgccgcgaaa ggacgccccg cggatcaccc ggtcatcgtg cacgtacagg atgcgcgcgc
     1561 gctcgacacc tgggcgcgcg aagttcccgc cgccgcgcac cggctcgcgc gcgtgttctg
     1621 gccggggccg ctgacgctgg tgctgaagcg cggcgcccgc gcgcacgaga gcctgaccgg
     1681 cggccaggat acggtcggcc tgcgctgccc cgcgcatccg tgggcacgcg cgctgctggc
     1741 ggcgttctgt gccgcgcgcg gtgatccggc cgccgcgctt gcggcgccga gcgcgaacag
     1801 cttcggccgc atcagcccat cgcgcgccga gcacgtgcgc gcggacctgg gcgaaaggcc
     1861 gacaggcaag gtcgacctga tcctcgacgg cggcgcatgc ccgatcggta tcgaatcgac
     1921 gatcgtcgac ctgtccggcg cgccgcc
//
LOCUS       DBNHBY010000642         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_180446, whole genome shotgun sequence.
ACCESSION   DBNHBY010000642 DBNHBY010000000
VERSION     DBNHBY010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_180446"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="AC16HB_15410"
     CDS             complement(<1..1145)
                     /locus_tag="AC16HB_15410"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HIR5608226.1"
                     /translation="MLRPSRAGRSRIQGCGRFWASGRAAVIPNRQRVRAVFSLDQRKS
                     DWQVVSGAVIKRNANEMRSLQRICAWQRAADFSPVGAFKTNRPQAHDRTGPLPRSPSR
                     ELRILRQLITVRTHAVAEPSPADRQEGHLLMHPSAAEVDMIAATSRVPGSEAAPDEKD
                     RHWYRHVYQGEHVPQLTFRAVAVGAVLGMLMSVSNLYTTMKVGWSFGVAITSCVMSFV
                     IWNALRGISGGRLTPMSILENNCMQSTASAAGYSTGATIATAFGALLILDPSHRHQPW
                     WIVASFTLVTGLMGVFMAIPLKRQMINHEQLPFPSGIAAATTLRSLYERGAVALKKAY
                     SLVAALVIGTAVGVLNTAEDQCAALGRFFVWMREHVFDVHLPDQVPE"
     gene            1311..>2265
                     /locus_tag="AC16HB_15415"
     CDS             1311..>2265
                     /locus_tag="AC16HB_15415"
                     /inference="COORDINATES: protein motif:HMM:NF020663.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608227.1"
                     /translation="MALTTRNLLRAAVRSSLAVAVGLAAWIAVGPAQAFDRDSLIWKK
                     CTPCHAASADGRIARVEDLRTTPEEWTVIVDRMRRLHGMEIKQGEMDRMLKELTATQI
                     LTPREQARVAYLSLWHNSQQVESPADKDEEKLFATCVRCHSAGKIFSYRMTTENWAKV
                     RDFHLFAVPSVLFQMREMRWVTEADAMLALLAKKLPYEKPWSAPAAKLDGTWGIFGYE
                     PGRGPYRGEARITDAGNAEYRLTGSVTYADGTSEAFAGEATLYGGYALRTRTKNNGFA
                     SHGAFSLAGDELRGEWHLPAPNFRTSGATWLRDGGAARVARV"
BASE COUNT          395 a          763 c          754 g          353 t
ORIGIN      
        1 tgctcgggca cctgatcggg caggtgcaca tcgaagacgt gctcacgcat ccagacgaag
       61 aatcggccca gggccgcgca ctggtcctcg gcggtgttca gcacgcccac cgcggtgcca
      121 atgacgagcg ctgccaccag cgaataggcc ttcttcagcg ccacggcgcc gcgctcgtac
      181 aggctgcgca gcgtggtcgc cgcggcgatg ccagacggaa acggcaactg ctcgtgattg
      241 atcatctggc gcttcagcgg gatcgccatg aagacgccca tcagccccgt gaccagcgtg
      301 aacgacgcga caatccacca cggctggtgg cggtggctcg ggtcgaggat cagcagcgca
      361 ccgaaggcgg tggcgatcgt cgcgcccgtg gagtagcccg cggccgacgc ggtcgattgc
      421 atgcagttgt tctccaggat cgacatcggc gtaagtcgtc cgccggagat gccgcgcaag
      481 gcgttccaga tcacgaacga catcacgcaa gatgtgatcg cgacgccgaa agaccagccg
      541 accttcatcg tcgtatacag gttcgacacg gacatcagca tcccgagcac cgcgccgacc
      601 gcgaccgccc ggaacgtgag ctgcggcacg tgctcgccct ggtagacgtg ccggtaccag
      661 tggcggtcct tttcgtcggg cgcggcctcg gatccgggga cgcgtgacgt cgcggcgatc
      721 atgtcgacct ccgcagcgct tggatgcatg agcagatgtc cttcctgacg gtccgccggc
      781 gacggctcgg ccacggcatg cgtgcgcacg gtaatgagtt gccgcaggat gcgcaattct
      841 cggctcggcg aacgcggcag cggcccggtg cggtcgtgag cctgcggtcg atttgtcttg
      901 aaagcaccta ccgggctgaa atcggccgca cgctgccatg cgcagatgcg ttgcagcgat
      961 cgcatctcat tcgcgttgcg tttgattacc gcaccgctca cgacctgcca atccgatttt
     1021 ctttgatcta gagaaaaaac ggcgcgcacg cgttgccgat tcgggataac ggccgctcga
     1081 cccgacgccc agaatcgccc gcacccctga attctgctgc gtcccgcacg ggacggccgc
     1141 agcacacgcg gaggaatcga tgctgcgacg aagcgcgctc gtcagtgtgc cggcgattcc
     1201 acgcgcggcg ttcgcatcga tggcgcgccg agctgccgcg tcgcatcgcg cggccgaatg
     1261 acctccggct ttcctcccgt tccacacgct caacgcatcg gagacgcgtc atggcactga
     1321 ctacccgcaa cctcctccgt gctgcagtcc gatcgtcgct ggccgtcgcc gtcggcctcg
     1381 cggcgtggat cgccgtcggc ccggcgcagg cgttcgaccg cgactcgctg atctggaaga
     1441 aatgcacgcc gtgccacgcc gcgagcgcgg acggccggat cgcgcgcgtc gaggacctgc
     1501 gcaccacgcc ggaggaatgg accgtgatcg tcgaccgcat gcggcggctg cacggcatgg
     1561 agatcaagca gggcgagatg gaccggatgc tgaaggaact cacggccacg cagatcctga
     1621 cgccgcggga gcaggcgcgc gtcgcttatc tgagtctctg gcacaactcg caacaggtcg
     1681 agtcgccggc cgacaaggac gaggaaaagc tcttcgcgac ctgcgtgcgc tgccactcgg
     1741 cgggcaagat cttctcgtac cggatgacga cggagaactg ggccaaggtg cgcgacttcc
     1801 acctgtttgc cgtgccgtcg gtcctgttcc agatgcgcga gatgcgctgg gtcacggagg
     1861 ccgacgcgat gctcgcgttg ctggcgaaga aacttccgta cgagaagccc tggtcggcgc
     1921 cggccgcgaa gctggacggc acctggggga tcttcggtta cgaaccgggc cgcggtccgt
     1981 accggggcga agcgcgcatc accgatgccg gcaatgccga gtaccggctc acgggcagcg
     2041 tcacctacgc cgacggaacc tcggaagctt tcgcgggcga agccacgctg tatggcggct
     2101 acgcgctgcg cacgcgcacg aagaacaatg gcttcgccag ccacggcgca ttcagcctgg
     2161 cgggcgatga gctgcgcggc gaatggcacc tgccggcgcc gaacttccgc acctccggcg
     2221 cgacctggct gcgcgacggc ggcgccgcca gggtcgcgcg cgtcg
//
LOCUS       DBNHBY010000643         2377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_586686, whole genome shotgun sequence.
ACCESSION   DBNHBY010000643 DBNHBY010000000
VERSION     DBNHBY010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_586686"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="AC16HB_15420"
     CDS             complement(<1..1249)
                     /locus_tag="AC16HB_15420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5608228.1"
                     /translation="MRRDAEPGDGDAKLRDILSRTTAAAQAVSSARPGRVFEDLALAL
                     ARILATDAALIAQFVDERRQRMRTLALCMEGRLLHEVEYNVAHTPCRHIVGRESRFIS
                     SGVGAEFAPGTIFAASGFDAYAGYSIPDSAGEQAGIIVALDRKPIADRELTEALLKIF
                     ATRAGAEIDRLRAEAALRASETSYRAIFEAAEDAIFVHDRDTGRILDVNSKACSAYGW
                     PREEMLACSIEDFSADPPPCSRADAMRHIEAAKSGAPVRFEWHRRNRDGSLHWDEVVL
                     KSAVIDGAPRILAFTREITERKQALEALQQREEQYREIFNASADALVLWNKDLRIVDV
                     NPAFLRTYGFARGDIVGAAYPAHFPREYVDDRLQLVRRALAGEACELQTRAYRANGEP
                     FDVDLRVIPIRHRGEPHALAIARD"
     gene            complement(1353..2063)
                     /locus_tag="AC16HB_15425"
     CDS             complement(1353..2063)
                     /locus_tag="AC16HB_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608229.1"
                     /translation="MVSARVLVVDDDADVRRMLAEYLGAHDYAVDVAANGDEMRAAIA
                     RNVPDVVLLDVGLPGEDGLTLARHLRERYSVGIIMVTAADGVVDRVVGLEVGADDYIA
                     KPFDPRELRARLKSVLRRLRPDGAQAVTAERTRPRIGRCMLDLDARRLLDENGVDVPI
                     TAMEFDLLKTFVEHPNKVLTRDRLLTLTRNREWEPFDRSIDIRITRLRRKVEANPEKP
                     LAIQTVRGAGYMFVPPVD"
     gene            2188..>2377
                     /locus_tag="AC16HB_15430"
     CDS             2188..>2377
                     /locus_tag="AC16HB_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608230.1"
                     /translation="MRCIGNAIRKPTMQTRHDAAADPEYALDAWAQRAGAANGFADAD
                     IALGRLPLRAGNAEFDRIA"
BASE COUNT          374 a          840 c          798 g          365 t
ORIGIN      
        1 tgtcacgcgc gattgccagc gcgtgcggct cgccacggtg gcggatcggg atcacccgca
       61 aatcgacgtc gaacggctcg ccgttggcgc ggtaggcacg cgtctgcagt tcgcaggcct
      121 cgcccgccag cgcgcggcgc acgagctgca gccgatcgtc gacgtactcg cgcgggaagt
      181 gcgccgggta cgcggccccg acgatgtcgc cgcgcgcgaa gccgtaagtg cgcaggaacg
      241 ccgggttgac gtcgacgatc ctgaggtcct tgttccacag caccagcgcg tcggcgctgg
      301 cgttgaagat ctcccggtac tgctcctcgc gctgttgcag cgcctcgagc gcctgcttgc
      361 gttcggtgat ctcgcgcgtg aacgcgagga tgcgcggcgc gccgtcgatc acggcggact
      421 tcagcaccac ctcgtcccag tgcaggctgc catcgcggtt gcggcgatgc cattcgaagc
      481 gcacgggtgc gccgctcttg gccgcctcga tgtgccgcat cgcgtcggcc cgcgagcacg
      541 gcggcggatc ggcactgaag tcctcgatgc tgcaggcgag catctcctcg cgtggccagc
      601 cgtaagcgct gcacgccttc gaattgacgt cgaggatgcg gccggtatcc cggtcgtgca
      661 cgaagatcgc atcctccgcc gcctcgaaga tggcgcggta actggtctcc gacgcgcgca
      721 aggccgcctc ggcgcgcagc cggtcgatct cggcaccggc acgcgtggcg aagatcttca
      781 gcagcgcctc ggtgagttcc cggtccgcga tcggcttgcg gtccagcgcc acgatgatgc
      841 cggcctgctc cccggcgctg tccgggatcg aatagccggc ataagcgtcg aagccgctgg
      901 cggcgaagat cgtgcccggc gcgaactcgg cgccgacgcc cgaggagatg aaccgcgact
      961 cgcgcccgac gatgtgccgg cacggcgtgt gcgccacgtt gtactcgacc tcgtgcaaca
     1021 ggcgcccttc catgcacagc gccagcgtgc gcatgcgctg tcgtcgctcg tcgacgaatt
     1081 gggcaatcag cgcggcatcg gtggcgagga tgcgcgccag cgccagcgca aggtcctcga
     1141 acacgcgccc ggggcgcgcg ctcgacactg cctgtgcggc cgcggtcgtg cgcgacagga
     1201 tgtcgcgcag cttggcgtcg ccgtcaccgg gttcggcatc tcgtctcatg cgtcgagtgt
     1261 aggagtgcag ccgcgcaccg gcacggtcga ttacacaact gaaacacggc gatacgcgcc
     1321 cgaaacctct gtcatccggg caccgccgcc gctcagtcca ccggcggcac gaacatgtag
     1381 ccggcacccc gcacggtctg gatcgcaagc ggcttttcgg gattggcctc gaccttgcga
     1441 cgcaggcgcg tgatgcgaat gtcgatcgag cggtcgaacg gctcccattc gcggttgcgc
     1501 gtcagcgtca gcaggcggtc acgcgtcaga accttgttcg ggtgctcgac gaaggtcttc
     1561 agcaggtcga actccattgc ggtgatcggt acgtcgacgc cgttctcgtc gagcagccgg
     1621 cgcgcatcga ggtcgagcat gcaccggccg atgcgcggcc gcgtacgctc ggccgtcacg
     1681 gcctgcgcgc cgtccggccg cagacgccgc agcacgctct tcaggcgcgc gcgcagttca
     1741 cgcggatcga acggcttggc aatgtagtcg tcggcgccga cctcgaggcc gaccacgcgg
     1801 tcgaccacgc cgtccgccgc ggtcaccatg atgatgccga cgctgtagcg ctcgcgcaga
     1861 tggcgcgcga gcgtcagtcc gtcttcgccc ggcaggccga cgtcgagcag caccacgtcg
     1921 ggcacgttgc gcgcgatggc ggcgcgcatc tcgtcaccgt tggccgcgac atcgacggcg
     1981 tagtcatgcg cgccgagata ttccgccagc atgcggcgca cgtcggcatc gtcgtcgacc
     2041 accagtaccc gcgcgctcac catgtcggcc gcctcccgac gattcacccg tgcacaagta
     2101 tagaaacgcc gcgggcaaac cggatcggta cctgccgtta cacagcgttg cgcaactgaa
     2161 agcgccgcga taccggcgct gcctacagtg cgctgcatcg gcaacgcaat tcgaaagcca
     2221 acgatgcaga cccgccacga cgccgcagca gatcccgaat acgcgctcga cgcctgggcg
     2281 cagcgcgccg gcgcagccaa cgggttcgcc gacgcggaca tcgcgctcgg ccggttgccg
     2341 ctgcgggccg gaaacgcgga attcgatcgc atcgcgc
//
LOCUS       DBNHBY010000644         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_112614, whole genome shotgun sequence.
ACCESSION   DBNHBY010000644 DBNHBY010000000
VERSION     DBNHBY010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_112614"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="egtB"
                     /locus_tag="AC16HB_15435"
     CDS             complement(<1..854)
                     /gene="egtB"
                     /locus_tag="AC16HB_15435"
                     /EC_number="1.14.99.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0044875 - gamma-glutamyl hercynylcysteine
                     sulfoxide synthase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR5608231.1"
                     /translation="MRPAPKPKPQTQRASHDRYLAIRATTEALAAPLSAEDCAIQSMP
                     DASPVKWHLAHTTWFFETFVLAPHVPGYRIFDPAFRVLFNSYYNAIGERHPRPERGLL
                     SRPGLDEVLAYRRHVDAAMHGLLARGVTPDLADLIELGLHHEQQHEELIVTDLKHLLS
                     RNPLKPAYQKHWPLTSIRAREPHWIACDGGMHEIGHAGDDFAFDNETPRHRVWLQPYA
                     VASHPVTHGNFIEFIDDGGYRRPELWLAAGWDAVTTRGWQAPLYWERRDGTWYTFTLH
                     GEVAVDPHT"
     gene            complement(940..>1785)
                     /locus_tag="AC16HB_15440"
     CDS             complement(940..>1785)
                     /locus_tag="AC16HB_15440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5608232.1"
                     /translation="LGGGFIGLEFAQMFRRFGSEVTVLEGMARLAPRDDEDVSEALRK
                     VLEEDGIRIECDARVERVDGRAGDIGVHYAQGGTTTSVRGSHLLVSVGRRPNTDDLNL
                     AAAGVVTERGYVTVDATLATNVPGIYAVGDVKGGPQFTHIAYDDFRILRDRWVNHLDA
                     RLGDRLVPSTVFTDPQLAAVGLNETEARKRGLDIRVAKIGMNGFARALEMDESRGFIK
                     VILDAKSGQILGCTVFGIEGGEIMSMVQIAMIGKLPYTVLKETIFAHPTLAEGLNNVF
                     LALDA"
BASE COUNT          270 a          609 c          610 g          296 t
ORIGIN      
        1 gtgtgcggat cgaccgccac ctcgccgtgc agcgtgaacg tgtaccacgt gccgtcgcgg
       61 cgttcccagt agagcggcgc ctgccagccg cgggtggtga ccgcgtccca gccggccgcg
      121 agccaaagct cggggcgccg gtagccgccg tcgtcgatga actcgatgaa gttgccgtgc
      181 gtgaccgggt gcgacgcgac ggcgtacggc tgcagccaga cgcgatggcg cggcgtctcg
      241 ttgtcgaagg caaagtcgtc gcccgcgtgt ccgatctcgt gcatgccgcc atcgcacgcg
      301 atccagtgcg gttcgcgcgc tcggatcgac gtgagcggcc aatgcttctg gtaagcgggc
      361 ttgagcgggt tgcgcgacag caggtgcttc aggtccgtga cgatcagttc ctcgtgctgc
      421 tgctcgtggt gcaggccgag ttcgatcagg tcggcgaggt cgggtgtcac cccgcgcgcc
      481 agcaggccgt gcatcgctgc gtcgacgtgc cggcgatacg cgagcacttc atccagaccc
      541 gggcgcgaca gcaggccgcg ctcgggccgc ggatgccgct cgccgatcgc gttgtagtac
      601 gaattgaaga gcacgcggaa ggccgggtcg aagatccggt agccgggcac gtgcggcgcg
      661 agcacgaagg tctcgaagaa ccaggtcgtg tgcgcgaggt gccacttcac cgggctcgca
      721 tccggcatcg actggatcgc gcagtcctcc gccgacagcg gcgcggcaag cgcctccgtc
      781 gtcgcgcgaa tcgccagata gcgatcgtgg ctcgcgcgtt gggtctgcgg cttcggtttc
      841 ggtgcgggtc tcatcgctgc ctccgcaggt tcgaaatgcg gtatggaaac ggtaacaagg
      901 cgcggcgccg gcgcgatgac gcgaacgcgg cgcgcccgtc tacgcgtcga gcgcgaggaa
      961 tacgttgttc agcccctccg ccagcgtcgg atgcgcaaag atcgtctcct tcagcacggt
     1021 gtacggcagc ttgccgatca tcgcgatctg caccatcgac atgatctcgc cgccctcgat
     1081 gccgaacacg gtgcagccga ggatctggcc gctcttcgcg tcgaggatga ccttgatgaa
     1141 gccgcgcgac tcgtccattt ccagcgcacg cgcaaacccg ttcatgccga tcttcgccac
     1201 gcggatgtcg aggccgcgct tgcgtgcttc cgtttcgttc aggccgaccg cagcgagctg
     1261 cgggtccgtg aacaccgtgc tcggcaccag ccgatcgccg agccgggcgt cgaggtggtt
     1321 cacccagcga tcgcgcagga tgcgaaagtc gtcgtacgcg atgtgcgtga actgcggtcc
     1381 gcccttgacg tcgccgaccg cgtagatgcc gggcacgttg gtcgcgagcg tcgcatcgac
     1441 ggtcacgtag ccgcgctcgg tgacgacgcc ggcggcggcg aggttcaagt cgtcggtgtt
     1501 cggccggcgc ccgaccgaca ccagcagatg cgagccgcgc accgacgtcg tcgtgccgcc
     1561 ctgcgcgtag tgaacgccga tgtcgcccgc ccgcccgtcg acgcgctcga cccgcgcgtc
     1621 gcactcgatg cggatgccgt cctcctcgag caccttgcgc agcgcttcgc tcacgtcctc
     1681 gtcgtcgcgc ggcgcgagcc gcgccatgcc ttcgagcacc gtcacttcgc tcccgaaacg
     1741 gcggaacatc tgcgcgaatt cgagcccgat gaagccaccg ccgag
//
LOCUS       DBNHBY010000645         3355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1130056, whole genome shotgun sequence.
ACCESSION   DBNHBY010000645 DBNHBY010000000
VERSION     DBNHBY010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1130056"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="AC16HB_15445"
     CDS             <1..889
                     /locus_tag="AC16HB_15445"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5608233.1"
                     /translation="SPASRSPRVLLLLRIVALLPLRVVHAVGAAGGWLIYLLSKSYRR
                     QMHANLGLAQLDTPQIRRAAIAETGRQALESGWFWLRPAVDVAARVQDAHAGQLDALL
                     ADPRPLLLLTPHFGGFEAFAQYYATRPLAATRPMTVLYRVPRKAALRRVIETRGRTNL
                     LLAPADMRGVRMLARALKQRHTAGILPDQVPSRGEGVWVPFFNRPAYTMTLPARLAFG
                     SGARVVLVFCERLPTSRFRAHYVPLEEPLTGVPEQDAAALNRALERLIRARPEQYLWG
                     YARYKAPPGAPPVDAGSRA"
     gene            886..1767
                     /locus_tag="AC16HB_15450"
     CDS             886..1767
                     /locus_tag="AC16HB_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR5608234.1"
                     /translation="MSARLARLWIALIKATAHWRFATRARLGAWLGDLLWLVVRPRRR
                     IALANLRACFPEQSEAQRIAVARACFRLAARSAVDHSVLWRGSLEDVRRMVRCEGLER
                     TLALVREGPLIIMAPHFVGLDAGAIRLATETRAVSIYARQSSRAWDEALRAGRARFND
                     QLLIARSEQGAMRRALRALQSGLPMYYLPDMDHGVHNSIFVPFFGVEAATLPMLPRLA
                     KLANARVITAVTELRDDGYVVHLGEPWTDFPTDSVEHDTARMNREIERWVVRLPAQYL
                     WTHRRFKTRPPGAPALY"
     gene            1799..2650
                     /gene="dapF"
                     /locus_tag="AC16HB_15455"
     CDS             1799..2650
                     /gene="dapF"
                     /locus_tag="AC16HB_15455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR5608235.1"
                     /translation="MRVRFTKMQGAGNDFVMLDGIAQPFALNREQLRRLADRRFGVGA
                     DQILLVERAQRGDVDFRYRIFNADGGEVEQCGNGARCFVKFVRERRLTDRSAIRVETA
                     DGIIEPRLEDDGEVTVDMGEPRFDPAQVPFDANGLVARAEHGERLWPLRVAGAERWIG
                     VLSMGNPHAVQIVADVEAAPVTSEGPQIEHHPRFPHRVNAGYMQIVERHAIRLRVWER
                     GAGETLACGTGACAAAVSGIRRGVLDSPVAVQARGGRLRIAWQGLGHPVLMTGPAEKV
                     FEGEIDL"
     gene            2658..3317
                     /locus_tag="AC16HB_15460"
     CDS             2658..3317
                     /locus_tag="AC16HB_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR5608236.1"
                     /translation="MKSDDVAQYLSQHADFFEQHPELLGTLTVPHPQSGQAISLIERQ
                     SLLLRERIRALEAKIAEMLRHGEENDAIADKLVHWSRALLQQSSSALLPDTLIVELKR
                     LFSVPFAAVRVWGAAPAYAGIEATQPVSDDVLRLAASMQSPFCGSNVGFEVAHWMASD
                     EKQIASLAMMPLRVGGTPEAFGMLVLGSPDRDRFQITMGTSFLVRIAELASAAMSRLR
                     A"
BASE COUNT          475 a         1140 c         1217 g          523 t
ORIGIN      
        1 atcgcccgcc agccgatcgc cgcgcgtgct tctactactg agaatcgtcg ccctgctgcc
       61 gttgcgcgta gtgcacgccg tcggggcggc gggcggctgg ctcatctacc tgctgtcgaa
      121 gtcgtaccgg cgtcagatgc acgccaacct tgggctcgcg cagctcgata cgccgcagat
      181 ccggcgcgcg gcgatcgccg aaaccggccg acaggcgctc gagtccggct ggttctggct
      241 gcggccggcg gtggatgtcg ccgcgcgcgt gcaggacgcg catgcgggtc aactggacgc
      301 actgctggcc gatccgcgcc cgctgctgct gctgacgccg cacttcggcg gcttcgaggc
      361 gttcgcgcag tactacgcga cgcgtccgct ggcggcgaca cggccgatga cagtgctgta
      421 tcgcgtgccg cgcaaggccg cgctgcgcag ggtgatcgag acgcgcggcc gcaccaatct
      481 gctgctggcg ccggccgaca tgcgcggcgt acgcatgttg gcgcgcgcac tgaagcagcg
      541 gcacaccgct ggcattctgc ccgaccaggt gccgtctcga ggcgagggcg tatgggtgcc
      601 cttcttcaac cggcccgcct acacgatgac gctgccggcg cggctcgcct tcggcagtgg
      661 cgcccgcgtg gtgctggtgt tctgcgagcg cctgccgacc agccgctttc gcgcgcacta
      721 tgtgccgctg gaggagccgc tgaccggcgt gccggagcag gacgcggcgg cactgaaccg
      781 cgcgctcgag cgcttgatac gtgcgcggcc ggagcagtac ttgtggggct acgcgcgcta
      841 caaggcgccc cccggtgcgc cgccggtcga cgcggggagc cgggcatgag cgcccggttg
      901 gcgcgcttgt ggatcgcgct gatcaaagcg accgcgcact ggcgtttcgc cacccgagcg
      961 cgcctgggcg cgtggctcgg cgatctgctg tggctcgtcg tgcgtccgcg ccggcgcatc
     1021 gcgctcgcca acctgcgcgc gtgctttcct gagcagtcgg aggcgcagcg gatcgcggtc
     1081 gcacgcgcgt gctttcgcct ggcggcgcgc agcgcagtcg atcactcggt attgtggcgc
     1141 gggtcgctcg aggatgtgcg gcgcatggtc cgctgcgaag gtctcgaacg gacgctcgcg
     1201 ctcgtgcgcg agggtccgct gatcatcatg gcgccgcact tcgtcggtct cgatgccggc
     1261 gcgatccggc tggcgaccga aacgcgcgcg gtgtccatct acgcgcgcca gagcagtcgt
     1321 gcgtgggacg aggcattgcg ggcaggccgt gcccgcttca acgatcaact gctgatcgcg
     1381 cgcagcgagc agggcgcgat gcggcgcgcg ctgcgcgcct tgcagtccgg cctgccgatg
     1441 tactacctcc ccgatatgga tcacggcgtg cacaactcga tcttcgtgcc gttcttcggc
     1501 gtcgaggccg ccaccctgcc gatgctgccg cgcctcgcga agctcgcgaa cgcgcgcgtc
     1561 atcaccgcgg tcaccgaact ccgcgacgat ggctacgtgg tccacctcgg cgagccgtgg
     1621 acggacttcc cgaccgactc ggtcgagcac gacacggcgc gcatgaatcg cgagatcgag
     1681 cgttgggtgg tgcgactgcc ggcgcagtac ctgtggacgc accggcgttt caagacgcgt
     1741 ccgccgggcg ctcccgcgct gtactgagct cgaacaacgt gtaagcgata atcgtcgcgt
     1801 gagagtgcgc ttcaccaaga tgcagggcgc gggcaacgac ttcgtgatgc tggacggcat
     1861 cgcgcagccg ttcgcactca accgcgaaca actgcgccga ctggcggacc gtcgcttcgg
     1921 cgtaggtgcc gaccagatcc tgctggtcga gcgcgcgcag cgcggcgacg tggatttccg
     1981 ctaccggatc ttcaacgccg acggtggcga ggtcgagcag tgcggcaatg gcgcgcgctg
     2041 cttcgtcaag ttcgtgcgcg agcggcgcct gaccgaccgc agcgcgatcc gtgtcgagac
     2101 ggcggacggc atcatcgagc cgcggctcga ggacgacggc gaggtgacgg tcgacatggg
     2161 cgaaccgcgc ttcgaccccg cgcaggtgcc gttcgacgcg aacgggctgg tggcgcgcgc
     2221 cgagcacggc gagcggctgt ggccgctgcg cgtcgcgggc gcggagcgct ggatcggcgt
     2281 gctgtcgatg ggaaacccgc acgcggtgca gatcgtcgcc gacgtcgagg cggcgccggt
     2341 gacgagcgag gggccgcaga tcgaacacca tccgcgcttt ccgcacaggg tcaacgccgg
     2401 ctacatgcag atcgtcgaac ggcacgcgat ccgcctgcgc gtctgggagc gcggcgcggg
     2461 cgaaacgctc gcgtgcggca ccggcgcttg cgcggcggcg gtcagcggca tccgccgtgg
     2521 cgtgctcgat tcgccggtgg cggtgcaggc gcgcggcggc cggctgcgca tcgcgtggca
     2581 agggctcggc catccggtgc tgatgacggg accggccgag aaggtcttcg agggtgaaat
     2641 cgatctatag gggcgcaatg aaatccgacg atgtcgcgca gtacctgagc cagcacgccg
     2701 atttcttcga gcagcatccc gagttgctcg gcacgctgac cgtgccgcac ccgcagagcg
     2761 ggcaggcgat ctccctgatc gagcgccagt cgctgctgct gcgcgagcgc atccgcgcgc
     2821 tcgaggcgaa gatcgccgag atgctccgcc acggcgagga aaacgacgcg atcgccgaca
     2881 agctcgtgca ctggagccgt gcgctgttgc agcagtcaag ctctgcgctg ctgcccgaca
     2941 cgctgatcgt cgagctgaag cgcctgttca gcgtgccgtt cgcggccgtg cgcgtgtggg
     3001 gcgcggcgcc agcctacgcc ggcatcgagg ccacgcagcc ggtcagcgac gatgtgctgc
     3061 gactggcggc gagcatgcag tcgccgttct gcggctccaa cgtcggtttc gaggtcgcgc
     3121 actggatggc atccgacgag aagcagatcg cgagcctggc gatgatgccg ctgcgggtcg
     3181 gcggcacccc ggaggcgttc ggcatgctgg tgctcggctc gccggaccgc gatcggttcc
     3241 agatcacgat gggcacgtcg ttcctggtgc gcatcgccga actcgcgagc gccgcgatgt
     3301 cgcgcctgcg ggcatgagcc agcgccgggc catctcacga tggctcgctc ccccg
//
LOCUS       DBNHBY010000646         3392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1265153, whole genome shotgun sequence.
ACCESSION   DBNHBY010000646 DBNHBY010000000
VERSION     DBNHBY010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1265153"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC16HB_15465"
     CDS             <1..714
                     /locus_tag="AC16HB_15465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5608237.1"
                     /translation="GTMLVRNSRQNWVTKAQEWDYFHGIAAIKRVQSLYQGTLVAQGA
                     FSLYERKALLEVGGWSDRVGEDIVLTWAILARGWRVGHAEDACCFTRVPTTLPQFIRQ
                     RRRWARGVFEALRQHPEILRTPRLTQFFIFWNVLFPWTDLAFTLGFIPGIALAVFFGN
                     YLIAGPMTLALLPAALLLNMLMFGVGRRMFCAQGLHVRRNIAGFLLYTFGYALVLQPA
                     SVLGYASEVLNLRRSWGTK"
     gene            711..1637
                     /locus_tag="AC16HB_15470"
     CDS             711..1637
                     /locus_tag="AC16HB_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608238.1"
                     /translation="MIGRGTALALLFATAAACAQPAVTTEAAHTRDADGFAATRFAAG
                     YLPAYRSPQEYWGATAGNDHYAQDGWSRDGQRLLGRYRRLDAQTGAGLAVDAGAARVA
                     DRTRFVADAAWSVRLAPQTGVELIGARDWVETQRSIDDGITYDFVGASIEQGFGERFT
                     AIALAGHQRFSDGNTRNHLRARLIFALAPEHGISLQVRHRRFDADAITVPRRYFNPSR
                     YEETQAVVALRRRIASMPGWNVAGHVGGGRETIDRTEHKPTAAAELRLDGPLANGWHL
                     ALRAQYLRSAGGVEGIDYWYSLVGATLTVPLK"
     gene            complement(1645..2118)
                     /locus_tag="AC16HB_15475"
     CDS             complement(1645..2118)
                     /locus_tag="AC16HB_15475"
                     /inference="COORDINATES: protein motif:HMM:NF017079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Csu type fimbrial protein"
                     /protein_id="HIR5608239.1"
                     /translation="MIAALTLACAPATAQQGLSCTSQMSPGTLAFGNYVPSAPGGVSR
                     LLGNVVVDCSNTRNNPPNQIALRVSISAGQSGTTAQRHMTMPGSTTPLIYNLFMDAAY
                     TAVWPDSTTGRGEVLAVPPGQSAQLQMPVYGRIPAGQLTVRMGAYSDSLVLTLRY"
     gene            complement(2115..>3392)
                     /locus_tag="AC16HB_15480"
     CDS             complement(2115..>3392)
                     /locus_tag="AC16HB_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012786.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HIR5608240.1"
                     /translation="GVNQNLTVEGRFEVQSGVARVLGAAVDYGGRWGEIEAALAIDAL
                     DGTRGFAALGYRYQDFDNSVGVRWEQAQRGFRFAGETDLAPTPQRLVTVNASRRLSDR
                     WNVGGLLIDSRRPDRVRTRSANVAAFVALGSGWSAFLVVNRVDFDGRKTSSAGAFLTY
                     ALGPRTSANASVEGGTERRRTVGVLQALPIDEGWGYRVSASDTADGTRAEAAASVQTR
                     VATLSTEWVRDPQLGHAWRAGAAGSVGLIGSTPFIRRAINDSFALVQLDDFENVPILL
                     NNQVAGRTDARGQLILPRLNAYQPSEIRVDADDLPADARIGRDRDIIVAPFRSGVRIE
                     PDVKRVAAAVVRVRTARGVPVPAGAAVRVEPDAQATGIAQRGEFFVTGRPGRKRAMIE
                     LHGQSCSIDFELSVARGSAYQLLGPYECIGIQP"
BASE COUNT          522 a         1206 c         1142 g          522 t
ORIGIN      
        1 ggcacgatgc tggtgcgcaa ctcgcgccag aactgggtca ccaaggcgca ggaatgggac
       61 tacttccacg gcatcgcggc gatcaagcgc gtgcagtcgc tgtaccaggg cacgcttgtc
      121 gcacaaggtg cgttctcgct gtacgagcgc aaggcgctgc tcgaagtcgg cggctggtcg
      181 gatcgcgtcg gcgaggacat cgtgctgacc tgggcgatac tggcgcgcgg ctggcgcgtc
      241 ggtcatgccg aggacgcctg ctgcttcacg cgcgtgccga ccacgctgcc ccagttcatc
      301 cgccagcgcc gccgctgggc gcgcggcgtc ttcgaagcgc tgcgccagca cccggagatc
      361 ctgcgcacac cgcggctcac gcagttcttc atcttctgga acgtgctgtt tccgtggacc
      421 gatctggcgt tcacgctcgg cttcatcccg ggcatcgcgc tcgcggtctt cttcggcaac
      481 tacctgatcg cggggccgat gaccctggcg ctgctgccgg ccgcgctgct gctgaacatg
      541 ctgatgttcg gcgtcggcag gaggatgttc tgcgcgcagg gcctgcacgt acggcgcaac
      601 atcgccggct tcctcctcta cacgttcggc tatgcgctgg tgctgcagcc ggccagcgtg
      661 ctcggctacg catccgaggt gctgaacctg cgccgcagct ggggaacgaa atgatcggtc
      721 gcggcaccgc gctggcgctg ctgttcgcca ccgccgcggc ctgcgcgcag ccggccgtga
      781 ccacggaagc cgcgcacacg cgcgacgccg atgggttcgc ggcaacgcgc ttcgcagcgg
      841 gctacctgcc agcgtatcgc tcgccgcagg agtactgggg cgcgacggcg ggcaacgacc
      901 actacgcgca ggacggctgg tcgcgcgatg gccagcgtct gctcggccgc tatcgacggc
      961 tcgatgccca gaccggcgcc gggctggcag tcgatgccgg cgctgcacgc gtggccgatc
     1021 gcacgcgctt cgtggccgac gcggcctggt cagtgcggct cgcgccgcag acgggggtcg
     1081 agttgatcgg cgcgcgcgat tgggtcgaga cgcagcgcag tatcgacgac ggcatcacct
     1141 acgacttcgt cggtgccagc atcgagcagg gattcggcga acgcttcacc gcgatcgcgc
     1201 tcgcggggca ccagcgattc tccgatggaa acacgcgcaa ccatctgcgc gcgcgcctga
     1261 tcttcgcgct cgcgcccgag cacggcataa gtttgcaggt gcgccaccgc cgcttcgatg
     1321 ccgacgccat cacggtgccg cggcgctatt tcaatccgtc ccgctatgag gaaacacagg
     1381 cagtcgtcgc actgcgccgg cgtatcgcgt cgatgcccgg ctggaacgtc gccggccatg
     1441 tcggcggcgg gcgcgaaacg atcgatcgca ccgagcacaa gccgaccgcc gccgccgaac
     1501 tgcggctcga cgggccgctc gcgaacggct ggcatctcgc tctgcgcgcg cagtacctgc
     1561 gcagtgccgg cggagtggaa ggtatcgact actggtactc gctggtcggc gcgacgctga
     1621 ccgtgccgct gaagtagaca cgcctcagta gcgcagtgtc agtaccagag aatccgagta
     1681 cgcgcccatt ctgacggtca gctggccggc cgggatgcga ccatagaccg gcatttgcag
     1741 ctgtgcgctc tgcccgggcg gcacggcaag cacttcgccg cgcccggtgg tggagtctgg
     1801 ccagaccgcc gtgtacgcgg cgtccatgaa gaggttgtag atgagcggcg tcgtcgatcc
     1861 cggcatcgtc atgtgccgct gcgccgtcgt tccgctctgg cccgcggaaa tcgagacgcg
     1921 caacgcgatc tggttcggcg gattgttgcg cgtgttcgag cagtccacga cgacattgcc
     1981 gagcaaccgc gacaccccgc ctggtgccga cggcacgtag ttgccgaacg ccagcgtgcc
     2041 cggactcatc tgggacgtac acgagaggcc ctgttgcgcg gtcgccggcg cgcacgccag
     2101 ggtgagcgcg gcgatcaagg ctgaatgccg atacattcgt acgggccgag aagctgatac
     2161 gcgctgccgc gcgcgacgga cagctcgaag tcgatgctac aggactgccc atgcagttcg
     2221 atcatcgcgc gctttcgtcc cggccgaccg gtcacgaaga actcgccccg ctgcgcgatg
     2281 ccggttgcct gggcgtcggg ctcgacccgt accgccgcgc ccgcgggaac cggcacgccc
     2341 cgcgcggtgc gcacgcgcac gacggccgcg gccacacgct tgacgtccgg ctcgatgcga
     2401 accccgctgc ggaacggcgc gacgatgatg tcgcgatccc gcccgatacg cgcatcggcc
     2461 ggcaggtcat cggcatcgac gcggatttcg ctcggctgat acgcattgag ccgcggcagg
     2521 atcagttgcc cgcgcgcatc cgtgcgtccc gccacctggt tgttgagcag aatcggaacg
     2581 ttctcgaagt cgtcgagctg gaccagcgcg aagctgtcgt tgatcgcgcg cctgatgaac
     2641 ggggtcgagc cgatcagccc gacgctgccg gccgcccctg cgcgccatgc atggcccaac
     2701 tgcgggtcgc gcacccattc cgtcgacagc gtggccaccc gcgtctgcac gctcgccgcg
     2761 gcctccgcgc gcgtgccgtc tgcggtgtcg ctcgcgctga cccgatagcc ccagccctcg
     2821 tcgatcggca gcgcctgcag cacgccgacc gtgcggcgcc gctccgtgcc gccctcgacg
     2881 ctggcgttgg cgctcgtacg cggaccgagc gcatacgtaa ggaaagcacc ggcgctcgac
     2941 gtcttgcgcc cgtcgaagtc cacgcggttg acgacgagaa acgccgacca ccccgaaccc
     3001 agagcgacga acgcggcgac attggcgctg cgcgtgcgca cgcggtcggg gcggcgcgaa
     3061 tcgatcagca gcccgccgac gttccagcgg tccgacagtc gacgcgacgc gttgaccgtc
     3121 accagccgct gcggggtggg cgcgagatcg gtttccccgg cgaagcgaaa accgcgctgc
     3181 gcctgctccc agcgcacgcc gacactgttg tcgaaatcct ggtagcgata gcccagcgcc
     3241 gcgaacccgc gcgtgccgtc gagcgcgtcg atggcgagcg ccgcctcgat ctcaccccag
     3301 cggccgccat aatcgaccgc ggcgcccagc acccgcgcaa cgcccgactg cacctcgaag
     3361 cgcccctcga cggtcagatt ctgattgacg cc
//
LOCUS       DBNHBY010000647         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1804864, whole genome shotgun sequence.
ACCESSION   DBNHBY010000647 DBNHBY010000000
VERSION     DBNHBY010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1804864"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="AC16HB_15485"
     CDS             complement(<1..970)
                     /locus_tag="AC16HB_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608241.1"
                     /translation="MDFSITSSPKGAIEKVAAALVELAKGVEPEAKAENPASAANATA
                     ASAHADAVSELVHGGFVERILGETRTGVAAAPAQPPGERVDKGVETANLQTFARPHAP
                     VSEPLRPADTALPTDRNANSEFAVPASLLIPATLTSLQVERAASWPMTARSFDPDPKP
                     LHEARIHDRHPAPPPVAEELEEHEDEPAQEPDPAAEDDADPACSVLSENEDGDGWCEE
                     LTRAVRLALAAPLPPRALVLADEQWRRGRCVVLACPQGADPTGPAWAFVLWPRRRPDE
                     RWRRAASLPPQPLALFGLRVEASLRWFDPERAAHWRYARMIKEHHPR"
     gene            complement(977..1654)
                     /locus_tag="AC16HB_15490"
     CDS             complement(977..1654)
                     /locus_tag="AC16HB_15490"
                     /inference="COORDINATES: protein motif:HMM:NF042732.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HIR5608242.1"
                     /translation="MSKTRSTMRHANGSQDEMARRLSQAVTRTLTESLLLTLGSSEAP
                     DRPALTVERSPAPVAPARLAELQPGDAAVREATRAMYEQFLHAYRSIAQAQDTGSSID
                     DAGAAVAFFVAASFKALHGTDASRHTLAALERQLRGLVRLASRWDSISTAERQNYFEQ
                     MAIVGVLVAARAEQARKKNKADLRNVQGVAREYLRQLLGVDAEMIALGEAGLTLRKAV
                     GEARRAA"
     gene            complement(1787..2524)
                     /locus_tag="AC16HB_15495"
     CDS             complement(1787..2524)
                     /locus_tag="AC16HB_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608243.1"
                     /translation="MNLTAASLQGQPKSVDESRERGARGGAQFLTQQGEAFERALRAK
                     TEPGADARHQAVADAISRTDEGADAWRPVTAGIPAKVELRVRARLQDDDAAGDTPAAA
                     ASALAVAAVTPQTARPTEPAAAPVSVAIETVATGPRAAIEAALNSSPGPIVSPIGGTE
                     PAAVWEASVSEPNSIAVEVRAMRAEQPAAQQVQPGWMVAVTSSTVNADILSRHAPRLT
                     ERLRKQALGITDVRVEQDLQDAEEDDR"
     gene            complement(2521..3009)
                     /locus_tag="AC16HB_15500"
     CDS             complement(2521..3009)
                     /locus_tag="AC16HB_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608244.1"
                     /translation="MTKVLPKRHEAKQLCKLRSLRVQRAREGVAQAQAGVERAAAAVR
                     QRELLIADLRRSIAGLQQAVVTTLAPTLPRWSAVTTAQRERLADQLERAEYALLDDQH
                     ELEQAQEKLQAARSELTRALAREDAVRGLAEQTRRAHALNRERRAELEIEDQGKPTGV
                     SA"
     gene            complement(3006..>3350)
                     /locus_tag="AC16HB_15505"
     CDS             complement(3006..>3350)
                     /locus_tag="AC16HB_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HIR5608245.1"
                     /translation="EEVRSILDGHIVLSRALAQAHHYPAIDVLGSASRVFTRVAQRAH
                     QDAAAKMRSLMARFAEVEFLLRVGEYQAGSDPLADKAIERMPAVRALLQQRSDEATDF
                     DATIAQLQGVVK"
BASE COUNT          452 a         1178 c         1141 g          579 t
ORIGIN      
        1 tgcgcgggtg atgctccttg atcatccgcg cgtaccgcca gtgcgctgcg cgctccgggt
       61 cgaaccacct gagactcgcc tcgacgcgca ggccgaacag cgcgagcggc tgcggcggca
      121 gactggctgc gcgccgccag cgttcgtcag gccgccggcg cggccacagc acgaaggccc
      181 aggccgggcc ggtcggatcg gcaccttgcg ggcatgcgag tacgacgcag cgaccgcgac
      241 gccattgttc gtccgcgagg acgagcgcgc gcggtggaag cggtgccgcc aatgcaaggc
      301 gcacggcgcg ggtcaattcc tcgcaccagc cgtcgccatc ctcgttctct gacaagacac
      361 tgcatgccgg gtcggcgtcg tcttccgcgg cgggatctgg ctcctgcgct ggctcatctt
      421 cgtgctcttc gagctcctcg gctacgggcg gcggcgccgg gtggcggtcg tggatgcgcg
      481 cctcgtgcag tggcttcgga tcggggtcga agctgcgcgc ggtcatgggc cacgacgcgg
      541 cgcgctcgac ctgcaggctg gtcagcgttg ccgggatcag cagggaagcc gggacggcga
      601 actcgctgtt cgcgttccgg tccgtcggca gcgcggtatc ggcgggacga agcggctcgc
      661 tgaccggcgc atgcggtcga gcaaaggtct gcaggttcgc ggtttcgacg cccttgtcga
      721 cgcgctcgcc gggcggttgc gctggcgcgg cggcgacgcc ggtgcgcgtc tcgccgagga
      781 tgcgttcgac gaatccgcca tgaaccaact ccgagaccgc atccgcatgc gcgctcgcgg
      841 cagttgcgtt ggcggcactc gcggggttct cagccttggc ttctggctcg acgcctttcg
      901 caagctcgac gagcgcggca gcgaccttct cgatcgcgcc cttcggcgat gatgtgatgc
      961 tgaaatccat cgccggtcag gcggcgcggc gagcctcgcc caccgccttg cgcagcgtca
     1021 ggccggcctc cccgagagcg atcatctcgg catcgacgcc aagcagctgg cgcaggtatt
     1081 cgcgcgcaac gccctggacg ttcctcagat cggccttgtt cttcttgcgc gcctgttcgg
     1141 cccgcgccgc gaccagcacg ccgacaatgg ccatctgctc gaagtagttc tggcgttcgg
     1201 cagtcgaaat cgagtcccag cgcgatgcca gccgaaccag accacgaagc tgccgctcca
     1261 gagccgcgag cgtatggcga ctcgcgtcag tgccgtgcag cgccttgaag ctggcggcga
     1321 cgaagaaagc gaccgccgcg cctgcgtcgt cgatcgacga cccggtgtcc tgcgcctgtg
     1381 caatggatcg ataggcgtgc aggaactgct cgtacatcgc acgggtcgct tcgcgcacgg
     1441 ccgcatcgcc cggctgcagc tctgccaggc gcgccggcgc aaccggcgcg gggctccgct
     1501 cgacggtaag cgcagggcga tccggagcct cgctggagcc gagagtcagc agcagacttt
     1561 cggtcaatgt gcgcgtaacg gcctgggata gccgacgtgc catctcgtcc tgactgccat
     1621 tggcgtgtcg catggtcgag cgcgtcttgc tcatggttct ccctcgatct gatcgaaact
     1681 ctgccgcggc gcacatctcg cgactgaagc gcagccgtgt tcgggtgttg ggttgccgtg
     1741 cgtccggcga aggttcaaat cgatgcgccg ccaccgcacc gaatcgtcac cggtcgtctt
     1801 cctcggcgtc ctggaggtcc tgctcgacgc gcacgtcggt gatgccaagc gcctgcttgc
     1861 gcaggcgctc cgtcaggcgc ggcgcgtgtc gggagaggat gtccgcattc accgtcgacg
     1921 acgtgaccgc caccatccag cccggctgca cctgctgggc agccggctgt tccgcacgca
     1981 tcgcgcgcac ctcgacggca atcgaattgg gttcactcac cgaggcttcc cacaccgccg
     2041 ccggctccgt gccgccgatg ggcgacacga tcggccccgg gctggaattc aacgcggcct
     2101 cgatcgccgc tcgcggcccc gtggcgacgg tttcgattgc aacggagacc ggggcggcag
     2161 cgggttcggt ggggcgcgcg gtctgtggcg tgactgccgc cacggccagc gcggatgcgg
     2221 cggcggcggg cgtgtccccg gctgcgtcgt cgtcctggag tctggccctg acgcgcaact
     2281 cgaccttcgc cgggattccc gccgttactg ggcgccacgc gtcggcaccc tcgtctgtcc
     2341 gcgaaatcgc atccgcgacg gcttgatggc gcgcgtcggc gcccggttcg gtcttggcgc
     2401 gcagtgctcg ctcgaacgcc tcgccctgct gcgtcagaaa ctgcgcgccg ccgcgagcgc
     2461 cccgttcacg cgattcgtcg accgatttcg gctgcccctg cagagatgcg gcggtcaggt
     2521 tcatgcgctc actccggttg gctttccctg gtcctcgatc tcgagttcgg cgcgccgttc
     2581 acgattcagc gcgtgcgcgc gccgtgtctg ctccgccagc ccacgcaccg catcttcgcg
     2641 cgccagcgcg cgcgtcagtt cggatcgcgc cgcctgcagc ttttcctgtg cctgctccag
     2701 ttcatgctgg tcatcgagca gcgcatactc ggcgcgttcg agttggtcag cgagccgctc
     2761 ccgctgcgcc gtggtgaccg cggaccagcg cggcagcgtg ggcgcaagcg tggtgacgac
     2821 ggcttgctgc agacccgcga tcgaacgccg cagatccgca atgagcagtt cgcgttgcct
     2881 gacggccgcg gcggcgcgtt cgacgcctgc ctgcgcctgc gcaacacctt cgcgtgcgcg
     2941 ctgcacgcgc agcgaacgca gtttgcacag ctgcttcgcc tcgtgccgtt tcggcagcac
     3001 tttggtcact tcacgacccc ttgcagctgg gcgatggtgg cgtcgaagtc ggttgcctcg
     3061 tcggagcgtt gctgcagcag cgcgcgcacg gccggcatgc gctcgatcgc cttgtcggcc
     3121 agcggatcac tgcccgcctg atactcgcca acacgcagca ggaattccac ctcggcaaac
     3181 cgtgccatca gggaccgcat ctttgctgcc gcgtcctggt gcgcccgctg cgcgacgcgc
     3241 gtgaagacgc ggctggcgct gccgagcacg tcgattgccg gatagtggtg cgcctgcgcg
     3301 agtgcgcgcg acagcacgat atggccgtcg aggatcgagc gcacctcctc
//
LOCUS       DBNHBY010000648         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_722645, whole genome shotgun sequence.
ACCESSION   DBNHBY010000648 DBNHBY010000000
VERSION     DBNHBY010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_722645"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..820
                     /locus_tag="AC16HB_15510"
     CDS             353..820
                     /locus_tag="AC16HB_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5608246.1"
                     /translation="MKRYAELLADALTRVREIQPWDLTQQLALARPPLVLDVREPAEF
                     ARARIPGSINVPRGVLEQACEWEYDETVPELAGRNVESIVVVCRSGNRSLLAADMMLQ
                     MGFADVVSLKTGVRGWNDYEQPLVDGEGRTLEGDEAERLLAPRVRPDQRRPAR"
     gene            882..>1514
                     /locus_tag="AC16HB_15515"
     CDS             882..>1514
                     /locus_tag="AC16HB_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608247.1"
                     /translation="MPVHRLAALAVALASLPAVAQQPGATALTIYSSASPGAIAPDTY
                     RTPGRGGVPGYAVVRQEREIELARGRNEVKFTDVAALIDPTTVMFESLTDPKGTAVLE
                     QNFQFDLVSTEKLLQRFIDRPIAVEQVRGQSTESFTGTLLSRQGGVVLRRDDGSVQIL
                     PHNAGVKLPALPGGLITRPTLVWNVDARNAGRQRTRVAYQTQGITWWADYN"
BASE COUNT          244 a          501 c          546 g          223 t
ORIGIN      
        1 agcggttgaa cggctgcagc cggttgtcgc cgtcagtgta agaggtgccg gtcagtcgat
       61 ccgtacccgg cgcagctcca gcgcgtcgtt cggccacggc accacgtggg ggttcggccg
      121 catcacccgg gccggctggc ggcgctagag tggaatcgac ggcaggtcgg cctgcagcac
      181 cgcgagcgcg ttgcggtcga aggcgctcac gaagtgcggc ggcaatcagc gcgccggcca
      241 gcacggccgc gattcagcgt cccatcgtgc ttcttcgggt gcggcaggcg atgggcgcaa
      301 tccacgcccg atgtgatcca attccacgcg ttcaagcgtc gaggaaagat ggatgaagcg
      361 ctacgccgaa ctgctcgccg acgcgctgac gcgcgtgcgc gaaatccagc cgtgggactt
      421 gacgcagcaa cttgcgctgg cgcggccgcc gctcgtgctg gacgtgcgcg agccggcgga
      481 gttcgcgcgc gcccgcattc ccggatcgat caacgtgccg cgcggcgtgc tggagcaggc
      541 gtgcgaatgg gaatacgacg agacggtgcc cgagctggca ggtcgcaacg tggagtcgat
      601 cgtggtcgtg tgccgctccg gcaaccgctc gttgctcgcg gccgacatga tgctgcagat
      661 gggcttcgcc gacgtggtgt cgctgaagac tggcgtgcgc ggttggaacg actacgagca
      721 gccgctggtc gatggggagg ggcgcacgct cgaaggcgac gaggcggagc gcctgctcgc
      781 gccgcgcgtg cgaccggatc agcggcggcc ggcgcgttaa ccccgatcgg cgcgctccga
      841 cgtttattgc gcggtgttca ttcaccgcgc aggagacgtc gatgcccgtc caccgtctcg
      901 ccgcgctcgc ggtcgcgctc gcgagccttc cagccgtcgc acagcagccc ggcgccaccg
      961 cgctgaccat ctattcgagt gccagtccgg gcgcgatcgc gcctgacacg taccgcacgc
     1021 ccggccgcgg cggcgtgccg ggctacgcgg tcgtgcgcca ggagcgcgag atcgagcttg
     1081 cgcgcggccg caacgaagtg aaattcaccg acgtggcggc gctgatcgat cccaccacgg
     1141 tgatgttcga atcgctgacc gacccgaagg gcaccgcggt gctcgagcag aacttccagt
     1201 tcgacctggt tagcaccgag aagctgctgc agaggttcat cgaccgcccg atcgcggtcg
     1261 agcaggtgcg cggccagtcg accgagtcgt tcaccggcac gctgctgtcc aggcaaggcg
     1321 gcgtggtact gaggcgcgac gacggcagcg tgcagatcct gccgcacaac gcaggcgtga
     1381 agctgccggc gctgccgggc ggactgatca cgcggccgac gctggtatgg aacgtcgacg
     1441 caaggaacgc cggccggcag cgcacgcgcg tcgcttacca gacgcagggc atcacgtggt
     1501 gggccgacta caac
//
LOCUS       DBNHBY010000649         6484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1873182, whole genome shotgun sequence.
ACCESSION   DBNHBY010000649 DBNHBY010000000
VERSION     DBNHBY010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1873182"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="AC16HB_15520"
     CDS             complement(<1..324)
                     /locus_tag="AC16HB_15520"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR5608248.1"
                     /translation="MQVFRADLAHCAQVRGAVVVIDVLRAFTTAAHAFALGAQAILAI
                     DSIEALAALRRRYPDALAIGSKAGGAKVAGFDIGNSPSQLGHLDVAGRTLIMYTVGGV
                     RGIAAC"
     gene            complement(348..2546)
                     /locus_tag="AC16HB_15525"
     CDS             complement(348..2546)
                     /locus_tag="AC16HB_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR5608249.1"
                     /translation="MAATHQDQVLFHMTGKVNAEALPIEAESLRPAALAGYRDLTRLR
                     YDYPLVLIDDAVGSEFALPLSTTVDRLLADLAPRGIEGERLRRHVLRLERDIRVQVAQ
                     GAVGHLGELWEQAATRVAGADREVEEVLRLAAAALKFDGEVIDCSAATPARLVTHAWR
                     ATQADKASSFRAEIDRLKRKLTDILRAAHVHSEAGQRPEALRAAVGGPDADAFDFGMM
                     SKLISRNTPRDDLPAARRQRIEWALSVLRMQGFYPDPDAGAPALEYCFDNCAAAVGAY
                     RERLRSMVQLVKAMAIAELEGGGRYVEAKDDAFFARFDERALTAAELALFPDYLVCIP
                     PGRNDAPENAGLLDMLSSALPVKVLVLVDDLLEDTSIGTGHFAFGVRSARLATMAMGL
                     GGMFVLQSASSNLYALRDRIARGLTCRGPALFTVFAGTATPAAGLPPYLTAAAAMESR
                     AFPAFTYDAAAGENWAARFSLENNPEPDADWRIDAIEYADEALQRASERSAFTFADFA
                     LCDARYAHHFARVPRERWNTTMASVADWLALDEHEAAQRVPYLLAVDGDDVLHRVVVD
                     ARMMAAARRSLLLWHRLQEHGGVHDSHAERLLARERAAWEAQKLKEIEALRGAAPAAP
                     ADASAPVAAAAPAIAAPEPAPAEKPPSDEAWIETARCPSCGECTNVNDRMFAYNDNKQ
                     AYIKDITAGTYRQLVEAAESCQVAIIHPGKPRDPNEPGLAELIERAKPFM"
     gene            complement(2579..3562)
                     /locus_tag="AC16HB_15530"
     CDS             complement(2579..3562)
                     /locus_tag="AC16HB_15530"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5608250.1"
                     /translation="MAIPVSKTLIRLHEEHHKLEDYQGGVPRWCTGCGDNAILAAVQR
                     LCRDEDLAPERTVFVSGIGCSSRFPHYMKTYGFHGIHGRAFPLAEGVKMARPDLHVFV
                     NTGDGDCCSIGAAHWIHAIRYNMNLTMILHDNQVYGLTKKQASPTSPLGMKSNTTPRG
                     SVLEALNPLTVTLGVQNVSFVAQGVDWIPELLYDIISKAFHHKGLSFVRIIQRCPEWL
                     PKMFEPWLHDPNKTLLLTHEKGLAPSSEISRIYRNQRAHDPLNIHQAREIASTIDPIP
                     VGILYHNPDVPCYEDLRHAGGPRTPEAIRAGLEKELDKFTIWPDEKLAQAA"
     gene            complement(3571..5466)
                     /locus_tag="AC16HB_15535"
     CDS             complement(3571..5466)
                     /locus_tag="AC16HB_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5608251.1"
                     /translation="MSETALLEPATTSSTTRQAVVLNEHIVEVISDSGEGAQRCGQSL
                     ASIAAKSGNGIWTVEIIPAEIQPPARSVAGASGIRIRIGTKRVTNGGDETDLAVAFNE
                     QVLLGRVRAGELKKGATILLESMWREHRDPAIVKSYTETVDALLKDGYRVIEVPLERE
                     CRALMADARRGKNMFVLGILCSVYSFDLALAREQVALTFGKKEEKIIRVNQGLLEAGY
                     AWAEKNLDFKYSIPAQRASVPQIVVNGNTAIALGVLASGMDICAMYPITPATSASHYL
                     SECFERVGGLVHQAEDEIAACAFAIGASYAGRCAVTITSGPGYSLKQEGIGLAVMAEI
                     PLVVVNVQRGGPSTGQPTKAEQGDLLTSIFGSHGDAPKVVMSVSSIEDCFYAMITARK
                     IAETFNMVVIVLSDASLATAQQPFPRPNFSADWLAPPIDQTPVPEGAKPYDWDPVTGI
                     ARRFVPGQPGGMHTITGLAHDRASKVAYDPSSNEEGLRMRSLKLAALQKTLKTPLVYG
                     APAGDLLVVGWGSTQGVIEEAVDRLRAEGLKVSSLHLRFLQPMASGIGEIMKRFGKVM
                     TIEGNWSDRKEDELIDADNRRYSALAMMLRARYLVDVDCWSEARGQPIKPADVAAVLR
                     AKLQEGH"
     gene            complement(5471..>6484)
                     /locus_tag="AC16HB_15540"
     CDS             complement(5471..>6484)
                     /locus_tag="AC16HB_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5608252.1"
                     /translation="DMAKAGCEVIVYEAFHTPGGVLKYGIPDFRLPNEVIDAEIEKLR
                     GLGVRFECNTLVGRLFTIEQMVTEMGFHSVFIGTGAGYPSFMGIPGESLNGVLSANEL
                     LTRCNLMRARDFPNFDTPLQPGKHVAVIGAGNTAMDALRVSLRLGAESVHCIYRRSKK
                     EAPARVEEIHHAEEEGIDFNWLTSPIEILGDANNNVRALRCVRMELGEPDESGRRRPV
                     PVPGSEFEFVCDMVVFAIGTNANPILGQTSKLKLNKWGYIATDDNLATSIAGVYAGGD
                     IVTGAATVIEAMGAGRKAARSMKAWLGLRDSDVVYQPAPEAAARRFGIDRNEHNFARV
                     RIA"
BASE COUNT          949 a         2203 c         2202 g         1130 t
ORIGIN      
        1 gcaggcggcg atgccccgca ccccgcccac ggtgtacatg atcagcgtgc gcccggcgac
       61 gtccaggtga ccgagctgcg acggcgagtt gccgatatcg aagccggcaa ccttcgcgcc
      121 gccggccttc gagccgatcg ccagcgcatc gggatagcgg cggcgcaggg cggcgagcgc
      181 ttcgatcgaa tcgatggcga ggattgcttg cgcgccgagc gcgaaggcgt gcgcggcggt
      241 ggtgaacgcg cgcagcacgt cgatcacgac gaccgcgccc cgcacctgcg cgcaatgggc
      301 caggtcggcg cggaagacct gcattggccg ccccggagcg cgccgggtca catgaacggc
      361 ttggcgcgct cgatcagttc cgccaggccc ggctcgttcg ggtcgcgcgg cttgccgggg
      421 tggatgatcg cgacctggca cgactctgcg gcctccacca gttgtcggta ggttcccgcg
      481 gtgatgtcct tgatgtaggc ctgcttgttg tcgttgtagg caaacatgcg gtcattgaca
      541 ttggtgcatt cgccacagct ggggcagcgc gcggtttcga tccacgcttc gtccgagggc
      601 ggcttctcgg ccggcgcggg ttcgggcgcg gcgattgcgg gtgccgctgc cgccaccggc
      661 gccgaagcgt cggcgggggc tgccggcgcg gctccacgca gcgcctcgat ctccttgagc
      721 ttctgtgcct cccacgcggc cctttcgcgc gcaagcaggc gctcggcatg cgagtcgtgc
      781 acgccgccgt gctcctgcag ccgatgccag agcagcaggc tcctgcgcgc cgccgccatc
      841 atgcgtgcat cgacgacgac ccggtgcagc acgtcgtcgc cgtcgaccgc gagcaggtac
      901 ggcacccgct gcgcggcttc gtgctcatcg agcgccagcc agtcggcaac cgaggccatc
      961 gtcgtgttcc agcgctcgcg cggcacgcgc gcgaagtggt gggcatagcg cgcgtcgcac
     1021 agcgcgaagt cggcgaaagt gaatgcactg cgttcgctcg cgcgctgcag cgcctcgtcg
     1081 gcgtactcga tcgcgtcgat gcgccagtcc gcgtccggct ccggattgtt ctcgagcgag
     1141 aagcgcgcgg cccagttctc gcccgcggcc gcgtcatagg tgaacgccgg aaacgcgcgc
     1201 gattccatgg ccgcggcagc cgtcagatac ggcggcagcc ccgccgccgg tgtggccgtg
     1261 ccggcgaata cggtgaagag cgctggcccg cggcacgtca gcccgcgcgc gatccgatcg
     1321 cgcaatgcat acaggttcga gcttgccgat tgcagcacga acatgccgcc cagacccatc
     1381 gccatcgtcg ccagccgcgc gctgcgtacg ccgaaggcga agtggccggt gccgatcgag
     1441 gtgtcctcca gcaggtcgtc gaccagcacc agcaccttga ccggcagcgc ggaggacagc
     1501 atgtcgagca gcccggcatt ttccggcgcg tcgttgcgac cgggcggaat gcataccagg
     1561 taatcgggga agagcgccag ttccgccgcc gtcagcgcgc gctcgtcgaa gcgcgcgaag
     1621 aacgcatcgt ccttggcctc gacgtagcgg ccgccgcctt cgagttcggc aatcgccatc
     1681 gccttgacca gttgcaccat cgagcgcagg cgttcgcggt acgcaccgac cgcggccgcg
     1741 cagttgtcga agcagtactc gagcgcgggg gctcccgcat ccggatcggg atagaagccc
     1801 tgcatgcgca gcaccgacag cgcccactcg atccgctgcc ggcgcgcggc aggcagatcg
     1861 tcgcgcggcg tgttgcggct gatcagtttc gacatcatgc cgaagtcgaa cgcgtcggcg
     1921 tcagggccgc cgaccgcggc gcgcagcgct tcgggtcgct gtcctgcttc agagtgcacg
     1981 tgcgccgcgc gcaggatgtc ggtcaacttg cgcttcagcc ggtcgatctc ggcgcggaaa
     2041 ctgctggcct tgtcggcctg ggtggcgcgc cacgcatgcg tcaccagtcg tgccggggtg
     2101 gcggcgctgc agtcgatcac ctcgccgtcg aacttcagcg ccgcggccgc cagccgcagc
     2161 acttcctcga cctcccggtc ggcgccggcg acgcgcgtgg cagcctgttc ccacaattcg
     2221 ccgaggtggc cgaccgcgcc ctgcgcgacc tgcacgcgta tgtcgcgttc cagccgcagc
     2281 acatggcgtc gcagccgttc accttcgatg ccgcgcggcg cgagatccgc cagcaagcgg
     2341 tcgacggtcg tcgacaacgg cagcgcgaac tcgctgccga cggcatcatc gatcagcacg
     2401 agcgggtagt cgtagcgcag ccgggtaagg tcccggtagc cggccagcgc ggcagggcgc
     2461 aggctctcgg cctcgatcgg cagcgcctcg gcgttgacct tgccggtcat gtgaaacagc
     2521 acctggtcct gatgtgtcgc ggccatgtcc atggcgcttg cctcttctgt cgttccggtc
     2581 atgcagcctg cgccagcttc tcgtccggcc agatcgtgaa tttgtcgagc tccttctcca
     2641 gcccggcgcg gatcgcctcg ggcgtgcgcg gtcccccggc gtggcgcagg tcctcgtagc
     2701 agggcacgtc ggggttgtgg tacaggatgc cgaccggaat cggatcgatc gtcgaggcga
     2761 tctcgcgcgc ctggtggatg ttcagcggat cgtgcgcgcg ctggttcctg tagatgcgcg
     2821 agatctcgct gctcggcgcg agccccttct cgtgcgtcag cagcagtgtc ttgttcggat
     2881 cgtgcagcca gggttcgaac atcttcggca gccactcggg gcagcgctgg atgatgcgca
     2941 cgaacgacag gcccttgtgg tggaacgcct tgctgatgat gtcgtagagc agctccggta
     3001 tccagtccac accttgcgcc acgaacgaca cgttctgcac cccgagcgtg accgtcagcg
     3061 gattcagcgc ctcgagaacg ctgccgcgtg gcgtcgtatt gctcttcatg ccgagcgggg
     3121 aggtcggcga tgcctgcttt ttcgtcaggc cgtagacctg gttgtcgtgc aggatcatcg
     3181 tcagattcat gttgtagcgg atcgcgtgga tccagtgcgc ggcgccgatc gagcagcagt
     3241 cgccgtcgcc ggtgttcacg aacacgtgca ggtcggggcg cgccatcttc acgccttcgg
     3301 cgagcggaaa cgcgcggccg tggatgccgt ggaagccgta cgtcttcatg tagtgcggga
     3361 aacggctcga gcagccgatg cccgacacga acaccgtgcg ctccggcgcg aggtcttcgt
     3421 cgcggcacag ccgctgcacg gcggcgagga tcgcgttgtc gccgcagccg gtgcaccagc
     3481 gtggcacgcc gccctggtag tcctcgagct tgtggtgctc ctcgtgcagc cggatcagcg
     3541 tcttcgagac ggggattgcc atgtccatcg tcagtgccct tcctgcagtt tcgcgcgcaa
     3601 tacggccgcc acgtccgcgg gcttgatcgg ttgcccgcgc gcctcgctcc agcagtcgac
     3661 gtccaccaga tagcgcgctc tgagcatcat cgccagcgcc gagtagcggc ggttgtcggc
     3721 gtcgatcagt tcgtcttcct tgcggtcgga ccagttgcct tcgatcgtca tcaccttgcc
     3781 gaagcgcttc atgatctcgc cgatgccgga cgccatcggc tgcaggaaac gcaggtgcag
     3841 cgacgagacc ttcagtccct cggcgcgcag gcggtcgacg gcctcctcga tcacgccctg
     3901 cgtgctgccc cagccgacca cgagcaggtc gcccgcgggc gcgccgtata ccaggggcgt
     3961 cttcagcgtc ttctgcagcg cggccaattt gaggctgcgc atgcgcaggc cctcttcgtt
     4021 gctcgacggg tcgtacgcga ccttgctcgc gcggtcgtgc gcgagccccg tgatcgtgtg
     4081 catgccgccc ggctggcccg gcacgaagcg gcgggcgatg ccggtgaccg ggtcccagtc
     4141 gtacggcttc gcgccttcgg gcaccggcgt ctgatcgatc gggggcgcga gccagtccgc
     4201 gctgaagttc gggcgcggaa acggctgctg tgcggtcgcg agactggcgt cggacagcac
     4261 gatgacgacc atattgaatg tctcggcgat cttgcgcgcg gtgatcatcg cgtagaagca
     4321 gtcctcgatg ctggagaccg acatcaccac cttcggcgca tcgccgtgcg agccgaagat
     4381 cgaggtcagc aggtcgccct gttcggcctt ggtcggctgg ccggtgctgg ggccgccgcg
     4441 ctgcacgttg acgaccacca gcgggatctc cgccatcacc gccagcccga tgccttcctg
     4501 cttcagcgag taccccgggc ccgacgtgat cgtcaccgcg cagcggccgg cgtacgaggc
     4561 gccgatcgcg aacgcgcacg ccgcgatctc gtcctcggcc tggtggacca gcccgccgac
     4621 gcgctcgaag cattcggaca ggtagtgcga ggcggaggtc gccggcgtga tcgggtacat
     4681 cgcacagatg tccatgccgg aggcgagcac gccgagcgcg atcgcggtgt tgccgttgac
     4741 gacgatctgc ggaacgctcg cgcgctgcgc cggaatcgag tacttgaagt cgaggttctt
     4801 ctcggcccag gcgtagccag cctcgagcag cccctggttg acgcggatga tcttctcttc
     4861 cttcttgccg aaggtgagcg ccacctgctc gcgcgccagc gccagatcga agctgtagac
     4921 gctgcagaga atgccgagca cgaacatgtt cttgccgcgg cgcgcgtcgg ccatcagtgc
     4981 gcggcattcg cgctcgagcg ggacctcgat cacgcggtag ccgtccttca gcagcgcgtc
     5041 caccgtctcg gtgtacgact tgacgatcgc gggatcgcgg tgctcgcgcc acatgctttc
     5101 cagcaggatc gtcgcgccct tcttcaactc gcccgcgcgc acgcggccga gcagcacctg
     5161 ctcgttgaag gcgacggcga gatcggtctc gtcgcccccg ttggtcacgc gcttggtgcc
     5221 gatgcggatg cggatgccgc tcgcgccggc gacgctgcgt gccggcggct ggatttcggc
     5281 cgggatgatc tcgaccgtcc agatgccgtt gccgcttttc gcggcgatcg aggcgagcga
     5341 ctggccgcag cgctgtgcgc cttcgccgga atcgctgatc acctcgacga tgtgttcgtt
     5401 caggacgacg gcttgcctcg tggtggagga ggtcgtggca ggttcaagca gggctgtttc
     5461 gctcatgacg tcacgcaatc cgcacgcgcg caaagttgtg ttcgttgcga tcgatgccga
     5521 agcggcgcgc cgcggcctcc ggcgccggct gatagacgac atcgctgtcg cgcagcccga
     5581 gccaggcctt catcgagcgc gccgccttgc ggccggcgcc catcgcctcg atcacggtcg
     5641 cagcacccgt gacgatgtcg ccgcccgcat acacgccggc gatcgaggtc gcgaggttgt
     5701 cgtcggtggc gatgtagccc catttgttca acttcagctt ggaggtctgg ccgagaatcg
     5761 gattggcatt cgtgccgatc gcgaacacga ccatgtcgca cacgaactcg aactcgctgc
     5821 cgggcaccgg caccgggcgg cggcggccgg attcatcggg ttcgccgagt tccatcctca
     5881 cgcagcgcag ggcgcgcacg ttgttgttcg cgtcgccgag gatctcgatc ggactggtca
     5941 gccagttgaa gtcgatgcct tcttcctccg cgtggtggat ctcctcgacg cgggccggcg
     6001 cttccttctt cgagcggcgg tagatgcagt ggacgctctc ggcgccgagg cgcagcgaca
     6061 cgcgcaacgc atccatcgcg gtgttgccgg cgccgatcac cgcgacgtgc ttgcccggct
     6121 gcagcggcgt gtcgaagttg ggaaagtcgc gcgcgcgcat cagattgcag cgcgtgagca
     6181 gttcgttggc ggacagcacg ccgttcaggg actcgcccgg gatgcccatg aagctcggat
     6241 agcccgcgcc ggtgccgatg aacacggagt ggaagcccat ctcggtgacc atctgctcga
     6301 tcgtgaacag ccgcccgacc agcgtgttgc attcgaagcg cacgccgagt cccctcagct
     6361 tctcgatctc ggcgtcgatc acttcgtttg gcagccggaa gtcggggatg ccgtacttca
     6421 gcacgccgcc cggcgtgtgg aaagcctcgt agacgatgac ctcgcagccg gccttggcca
     6481 tgtc
//
LOCUS       DBNHBY010000650         3523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_45943, whole genome shotgun sequence.
ACCESSION   DBNHBY010000650 DBNHBY010000000
VERSION     DBNHBY010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_45943"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="AC16HB_15545"
     CDS             complement(<1..600)
                     /locus_tag="AC16HB_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608253.1"
                     /translation="MDMADKTASAGVPLSIVLAPTASGAAELSHPAAGLSLSQRKLLA
                     RIDGRRSLASLVGTETGLTGERLSRDGLRLLALGLAHTDEAFAVPTSTTIMRALAETE
                     STLPPPETLPPQSTSDRVVQPRRPRRAAWVWTGAVIASVVAVAALLWRAPSVPVPSND
                     LATNVAVPPAAAPAAQPPPTQAVQAGAVGTVEPPAAVEAA"
     gene            816..2006
                     /locus_tag="AC16HB_15550"
     CDS             816..2006
                     /locus_tag="AC16HB_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HIR5608254.1"
                     /translation="MNMIDRIVQFRAEIQAIRRDIHAHPELRYEEARTSDLVAGRLAD
                     WGIEVHRGLGGTGVVGTIRHGKSKKSIGLRADMDALPIQETNQFAHRSRHDHKMHACG
                     HDGHVAMLLAAAKYLADVKPFDGTVHLIFQPAEEGGAGAQRMIDDGLFDRFPCDAVFG
                     MHNWPNLAVGQFGLTAGPMMASGNQFEITVTGKGSHGAMPHLGIDPVFVAVQIVQGLQ
                     GIITRAKKPIDSAVLSVTIIKAGDATNVVPDCAVIAGTVRTFDDGVTDLIEERMSRIA
                     QLTAEAHGAAAAVKFERTYPPTVNHARETEFAASVMDDVVGAANVVRGIEPTMGSEDF
                     AFMLRAKPGCYVFIGNGDGSHRAAGHGLGPCMLHSSSFDFNDELIPIGATYWVRLVEK
                     FLGR"
     gene            2140..>3523
                     /locus_tag="AC16HB_15555"
     CDS             2140..>3523
                     /locus_tag="AC16HB_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608255.1"
                     /translation="MRPTVIRKDPASFRVPPHLADWGAARATFSWADARAALAGLPGG
                     ALNIAHEAVEVHARSAHADRVAIRWLGADGDAIQFTYADLSALSSRCANALRALGLQR
                     GDRVFLLLPRIPELYIAVLGALKAGLVVSPLFSAFGPEPIATRVNLGGGRALLTTRLL
                     YERKVAQVRAQMPTLEHVLLAGDQAETDVPAGTRALDPLLDAAGDAFATVATRPDEMA
                     LLHFTSGTTGKPKGAIHVHEAVVTHYATGRYALDLHDGDVFWCTADPGWVTGMSYGVI
                     APLVHGMTMIVDEAEFDPERWYRILQEQRVTVWYTAPTAIRMLMKAGVELARNFRLDS
                     LRFVASVGEPLNPEAVWWGFDALGLPIHDNWWQTETGGIMIANTPAFDIKPGSMGRPL
                     PGVDAFVVQRRREGESTVGVDIIDTADTEGELALAAGWPSMFRGYLNNEERYRKCFVE
                     SSQGRLYLTED"
BASE COUNT          556 a         1200 c         1256 g          511 t
ORIGIN      
        1 agctgcctcg accgccgccg ggggttcgac cgtgccgacc gctccggctt gcacggcctg
       61 agtcggcggc ggctgcgctg ccggcgcggc ggcgggcgga accgcgacgt tcgtcgccag
      121 gtcattgctt gggaccggca ccgacggcgc acgccacagc agtgcggcga ccgccaccac
      181 ggatgcgatc acggcgccgg tccacaccca cgcggcgcgg cgaggtctgc gcggctgcac
      241 gacgcgatcg gatgtggatt gcggcggcag cgtttcgggc ggcggcagcg tcgattccgt
      301 ctccgcgagc gcacgcatga tcgtcgtgct cgtcggcacg gcgaaagcct cgtcggtgtg
      361 tgccaggccg agcgccagca ggcgcaggcc gtcacgcgac agccgctcgc cggtcagtcc
      421 ggtctcggtg ccgacgaggc ttgcaagcga acgccggcca tcgattcggg ccagcagctt
      481 gcgctgcgac aggctcaggc ccgccgcagg atgcgatagc tcggccgcac ccgacgcggt
      541 cggcgcaagc acgatcgaca gcggcacgcc cgcgcttgcc gtcttgtctg ccatgtccat
      601 aggggcctcc ctcgctgccg gcgactaccg ctcttacagg gcaagcaaca tatcactcgc
      661 acgtcgccgg agcgcgccga cgatgctcaa tgcatagtcg gacgaatgga tgagtcaccg
      721 ggccgccacc gcgcgcacgc cggagacggg ccatggccgc gcgccggatg ccgcggtgac
      781 ttagcatccg ggatgcgcaa cgcagcgggg acgccatgaa catgatcgat cggatcgtcc
      841 agtttcgcgc cgagatccag gcgatccggc gcgacatcca cgcgcacccc gaactgcgct
      901 acgaggaagc ccgcaccagc gacctcgtgg cgggccggct tgccgactgg ggcatcgagg
      961 tgcatcgcgg gctcggcggc accggcgtgg tcggcacgat ccgccacggc aagtcgaaga
     1021 agtcgatcgg cctgcgcgcc gacatggatg cgctgccgat ccaggagacc aaccagttcg
     1081 cgcaccgctc gcggcacgac cacaagatgc acgcctgcgg ccacgacggg cacgtcgcga
     1141 tgctgctcgc cgcggcgaag tacctcgccg acgtgaaacc cttcgacggc accgtgcacc
     1201 tgatcttcca gccggccgag gaaggcggcg cgggcgcgca gaggatgatc gacgacgggc
     1261 tgttcgacag gtttccgtgc gacgcggtgt tcggcatgca caactggccg aacctcgccg
     1321 tgggccagtt cgggctgacc gcgggcccga tgatggcgtc gggcaatcag ttcgagatca
     1381 cggtgaccgg caagggctcg cacggcgcga tgccgcacct ggggatcgac ccggtgttcg
     1441 tcgcggtgca gatcgtgcag gggctgcagg gaatcatcac gcgcgccaag aagccgatcg
     1501 actccgcggt gctgtcggtg acgatcatca aggccggcga cgcaaccaac gtggtgcccg
     1561 attgcgccgt gatcgccggc accgtgcgca cgttcgacga cggcgtgacc gacctgatcg
     1621 aggagcggat gagtcgcatc gcgcaactga ccgccgaggc gcacggtgcc gcggccgcgg
     1681 tgaagttcga gcgcacctac ccgccgaccg tgaatcacgc gcgcgagacc gaattcgcag
     1741 cgagcgtgat ggacgacgtc gtcggtgcgg cgaacgtcgt gcgcggcatc gaaccgacga
     1801 tgggctccga ggatttcgcg ttcatgctgc gcgcgaagcc cggctgctac gtgttcatcg
     1861 gcaacggcga cggttcgcac cgcgccgccg gccacgggct ggggccgtgc atgctgcaca
     1921 gttcgagctt cgacttcaac gacgaactga tcccgatcgg cgcgacctac tgggtgcggc
     1981 tggtggagaa gttcctcggt cgctgacgcc gaacaacctc gcctgccacg gccgggcgat
     2041 cgacgacgcg cagttgatgg cggtcaatca gggccgggga tcggcttcta acgtgcgaat
     2101 gagcgtggcg cccgcgctgt ggccggcgcc gcgccgcgca tgcgtccgac cgtcatccgc
     2161 aaggaccccg cttcattccg cgtgccgccg cacctggcgg actggggcgc ggcgcgcgcg
     2221 accttctcgt gggccgacgc gcgtgctgcg ctggccgggt tgcccggtgg cgcgctgaac
     2281 atcgcgcacg aggccgtcga agtccacgcg cgcagtgcgc acgccgaccg ggtggcgatt
     2341 cgctggctcg gcgccgacgg cgacgcgatc cagttcacct acgcggacct gtcggcgctg
     2401 tcgagccgct gcgccaacgc gctgcgcgcg ctcggcctgc agcgcggcga tcgggtgttt
     2461 ctgctgctgc cgcggattcc ggagttgtac atcgcagtgc tcggcgcgct gaaggcgggg
     2521 ctcgtggtat cgccgctgtt ctccgcgttc gggccggagc cgatcgccac gcgagtgaac
     2581 ctcggcggtg gccgcgcgct gctgaccacg cggctgctgt acgagcgcaa ggtcgcgcaa
     2641 gtccgtgcgc agatgccgac gctcgaacac gtgctgctgg cgggcgacca agccgagacg
     2701 gatgtcccgg ccggcacgcg cgcgctcgat ccgctgctcg atgcggcggg cgacgcgttc
     2761 gcaaccgtcg cgacgcgccc ggacgagatg gcgctgctgc acttcaccag cggcacgacg
     2821 ggcaaaccga aaggcgcgat ccatgtgcac gaggcggtcg tcacgcacta cgcgaccggc
     2881 cggtacgcgc tcgacctgca cgacggcgac gtcttctggt gcacggccga cccgggctgg
     2941 gtgaccggaa tgagctacgg cgtgatcgcg ccgctggtgc acggcatgac gatgatcgtc
     3001 gacgaggccg agttcgatcc cgaacgctgg taccgcatcc tgcaggagca gcgcgtcact
     3061 gtctggtaca cggcaccgac cgcgatccgc atgctgatga aggccggcgt ggaactggcg
     3121 cgcaacttca ggctcgattc gttgcgcttc gtcgccagcg tcggcgagcc gctgaatccg
     3181 gaagcggtgt ggtgggggtt cgacgcgctc gggctgccga tccacgacaa ctggtggcag
     3241 acggagaccg gcggaatcat gatcgccaac acgcccgcgt tcgacatcaa gccggggtcg
     3301 atgggtcggc cgctgccggg cgtggacgcc ttcgtcgtgc agcgacgtcg cgagggcgag
     3361 agcacggtcg gtgtcgacat catcgacacc gccgacaccg agggcgaact ggcgctcgcc
     3421 gccggctggc cgtcgatgtt ccgcggttat ctgaacaacg aggagcgcta ccgcaagtgc
     3481 ttcgtcgagt cgagccaagg tcggctgtac ctgaccgagg aca
//
LOCUS       DBNHBY010000651         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1466889, whole genome shotgun sequence.
ACCESSION   DBNHBY010000651 DBNHBY010000000
VERSION     DBNHBY010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1466889"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="AC16HB_15560"
     CDS             complement(<1..1286)
                     /locus_tag="AC16HB_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5608256.1"
                     /translation="MKRVPGSLRAAATLVVVRDDADGMQVLLLRRIERSGDRSSGAWV
                     FPGGTLDRGDRALHALCRGIDDTAASARLGVEANGLDFYIAAIRECFEEAGLLYASDA
                     IGELTALPRSPTPELEQMRAAIRHGAMSIGEVCTRLASFLAADRLAYLSHWLTPPGLP
                     KRFDTRFFIAVAPPGQAASFDTHETLEHRWLRPADALAADLKLPNPTRHTLRALAGFG
                     SARECFVHAQQRRAIERIMPRIGVGAEGPRPVMPNEPAYAEIGRIDPDGTQGARCEIV
                     PGRRVRLSQRAWRVTANNGSVMTGPGTNTYFVGDAAACAVIDPGPDDPAHFEAVLVAA
                     PAPIRWILVTHTHKDHSPAALQLKARTGAPVFGMTACHPEWQDATFVPDRVLAHGERI
                     AIGAHATLRVIHTPGHASNHLCYLLEEEQTLFTGDHV"
     gene            complement(1320..1805)
                     /locus_tag="AC16HB_15565"
     CDS             complement(1320..1805)
                     /locus_tag="AC16HB_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR5608257.1"
                     /translation="MQLTTVRIDKPDDLNFILGQSHFIKTVEDLHEAIVQTVPQMKFG
                     IAFCEASGPALVRFSGNDDALVELARKNALALSCGHSFIVFMHGGFPVNVLNTIKAVP
                     EVCSIFCATANPAEVIVAETESGRGILGVIDGLKSRGVEADADIAARKQLLRRFGYKL
                     G"
BASE COUNT          289 a          656 c          642 g          298 t
ORIGIN      
        1 acgtggtcgc cggtgaagag cgtctgctcc tcctccagca ggtagcacag gtggttcgac
       61 gcgtgccccg gcgtgtgaat cacgcgcagc gtcgcgtgcg cgccgatggc gatgcgttcg
      121 ccatgcgcca gcacccggtc gggaacgaac gtcgcatcct gccattcggg gtgacacgcc
      181 gtcatgccga agacgggcgc gccggtgcgc gccttcagct gcagtgccgc cggagagtga
      241 tccttgtggg tgtgggtgac gaggatccag cggatcggcg ccggcgcggc gaccagcacc
      301 gcttcgaaat gcgcgggatc gtccgggccc gggtcgatca ccgcgcacgc ggcggcgtcg
      361 ccgacgaagt acgtgttcgt ccccgggccg gtcatcacgc tgccgttgtt cgcggtcacc
      421 cgccacgcgc gttgcgacaa gcgaacgcgt cggccgggaa cgatctcgca gcgcgcaccc
      481 tgcgtaccgt ccggatcgat gcggccgatc tccgcatatg cgggctcgtt cggcatcacc
      541 ggccgcggtc cctccgcacc gacgccgatg cgcggcatga tgcgctcgat cgcgcgccgc
      601 tgctgcgcgt ggacgaagca ttcgcgcgcg ctgccgaagc ccgccagcgc gcgcagcgtg
      661 tgacgggtcg ggttcggcag cttgagatcg gctgcgagcg cgtcggcggg gcgcagccag
      721 cgatgctcga gcgtctcgtg cgtgtcgaac gacgccgcct gacccggcgg cgcgacggcg
      781 atgaagaagc gcgtgtcgaa ccgcttgggc agacccggcg gtgtcagcca atggctcaga
      841 tacgcgaggc gatcggccgc gagaaacgac gcgagccgcg tgcacacctc gccgatgctc
      901 attgctccat gccgaatcgc cgcgcgcatt tgctcgagtt ccggggttgg cgaccgcggc
      961 agcgcggtga gttcgccgat tgcatcggac gcgtacaaca gccctgcttc ctcgaagcat
     1021 tcgcggatcg ccgcgatgta gaaatccagc ccgttggcct ccacaccgag gcgagcgctg
     1081 gccgccgtat cgtcgatgcc gcggcacaag gcgtgcagcg cgcggtcgcc gcgatcgagc
     1141 gtgccgccgg ggaacaccca tgcgccgctg ctgcgatcac cgctgcgctc gatccggcgc
     1201 agcagcagca cctgcatccc gtccgcgtcg tcgcgtacga cgaccaaggt cgccgccgcc
     1261 cgcagcgatc cgggcacgcg cttcatggca accgcaacgg caggtccccg ggccggcgct
     1321 cagccgagct tgtagccgaa ccggcgcagc agctgcttgc gcgccgcgat gtcggcatcg
     1381 gcttccacgc ccctgctctt caggccgtcg atcacgccga ggatgccgcg cccgctctcg
     1441 gtctcggcga cgatcacctc ggccggattg gcggtcgcgc agaagatgct gcacacctcc
     1501 ggcacggcct tgatcgtgtt cagcacgttg accgggaagc cgccgtgcat gaacacgatg
     1561 aagctgtggc cgcaggacag cgccagcgcg ttcttgcgcg ccagctcgac gagcgcgtcg
     1621 tcgttgccgg agaaacgcac cagcgccggg ccgctcgctt cgcagaacgc gatgccgaat
     1681 ttcatctgcg gcaccgtctg cacgatcgcc tcgtgcaggt cttcgacggt cttgatgaag
     1741 tgcgactgac cgagaatgaa gttcaggtcg tccggcttgt cgatacgaac ggtcgtcagt
     1801 tgcatgctgc cctcctgcct ggtgccagga ggccagcata ccgacgctac ggccgcgcca
     1861 cgtgccagac gttgttgaaa ttgtc
//
LOCUS       DBNHBY010000652         3739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_993759, whole genome shotgun sequence.
ACCESSION   DBNHBY010000652 DBNHBY010000000
VERSION     DBNHBY010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_993759"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16HB_15570"
     CDS             <1..482
                     /locus_tag="AC16HB_15570"
                     /inference="COORDINATES: protein motif:HMM:NF017536.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HIR5608258.1"
                     /translation="EHHAAAQLPRIDRPGVAVHVVAGNAFGERAPVSVLSPTLYCSLK
                     FEAAAALTIPAEHPERAVYVVAGVVEVDGQLLSAGQMAVLEPGVEVTAQAKSQARAML
                     LGGAPVGERHIFWNFVSSSREKIEAAKAEWMRYSDVTARRVFGSIDGETEFIPLPVR"
     gene            complement(495..1577)
                     /locus_tag="AC16HB_15575"
     CDS             complement(495..1577)
                     /locus_tag="AC16HB_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5608259.1"
                     /translation="MTASSASLLSGGDLQRALDFLRAQRIEAPSMEQFARSAISGLAG
                     LVGADLVTLSYCDLRAGTRSVVTWPDESLSDEDVACFNRFFQSHPLVHYHSTHLGAGA
                     HRISDSAPDASFRRSALYADYYRRIGIDYVVAVPVHVDSARLISFVLNRARREFSDRD
                     CALLDTLRYQLGAEYMAQETRLRARRTLAQLNDVLMGAGMAVIVLDRTRRVVRSSEHA
                     LKWLAYAGFTGSVRAGERLPEPIDRWMRTRLEPSWAMFDPGPLALATAAHTLKLHLLQ
                     GEDALTLMLECVAADAPARDYGLTAREREILHWLAAGKSNAEIAAILAIRPRTVHKHL
                     ENIFRKLGVENRTAAALRALEIARSN"
     gene            1703..2113
                     /locus_tag="AC16HB_15580"
     CDS             1703..2113
                     /locus_tag="AC16HB_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608260.1"
                     /translation="MEVSTGSWLRRAVAPLWCAAALAGGVALAQSPLDGKAFDGVFLE
                     RGKTRGDADTLIFREGRFRSTACDKYGYGDAPYRIVSTDGGAIRFEVTTESPKYGKLE
                     WSGAVRGDKLDATAMMVQPGKAPVENWVVAGLRK"
     gene            2125..2496
                     /locus_tag="AC16HB_15585"
     CDS             2125..2496
                     /locus_tag="AC16HB_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5608261.1"
                     /translation="MNHAELKHFSQPDDVREFPHGRLELINIGGATVGRAIFEPGWRW
                     LTSVQPLANTKSCEAPHFQYHVAGVLMVKMDDGTELECHPGDVSLLPSGHDAWVVGDE
                     PAVVVDFQGMVDYAKAQHAHS"
     gene            2583..2786
                     /locus_tag="AC16HB_15590"
     CDS             2583..2786
                     /locus_tag="AC16HB_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIR5608262.1"
                     /translation="MWTMPWTEFEYPASMRHLSEPARAKAIEIANALLDEGMDEGKAI
                     RIAIAKAKEWAVQHGLPARDAEA"
     gene            2910..3605
                     /locus_tag="AC16HB_15595"
     CDS             2910..3605
                     /locus_tag="AC16HB_15595"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR5608263.1"
                     /translation="MNSPILRIPSRRRFNQAAVAATAWLVLPGARADDRADARGVVDK
                     ARITLESLVADKNMDALRAGLRQARGVLIFPQIIKGAFFVGGSGGTGALLVRNGETWT
                     GPAFYTIGSASIGVQFGGEAAEVAILVNSQKALDGLYGNRFKLGTDASIAAGPIGQGG
                     GVTFTSDMTSFSRSKGAFAGISLEGSVIDVRDSLNNAYYGRKVTPVEILAKRAVSNAQ
                     ADALRAALRAAAK"
BASE COUNT          597 a         1233 c         1350 g          559 t
ORIGIN      
        1 tcgagcatca cgcggccgcg cagttgccgc gcatcgatcg gcccggcgtc gcggtgcacg
       61 tggtcgcggg caacgcgttc ggcgagcgcg cgccggtcag cgtgctgtcg ccgacgctgt
      121 attgctcgct gaagttcgag gcggctgccg cgctgacgat ccccgccgaa catcccgagc
      181 gcgcggtgta cgtggtcgcc ggcgtggtcg aggtcgatgg gcagctgctc tccgccggcc
      241 agatggcggt gctggaaccg ggcgtcgaag tgacggcaca ggccaagagc caggcgcgcg
      301 cgatgctgct cggcggcgct ccggtcggcg agcggcacat cttctggaac ttcgtctcga
      361 gctcgcgcga gaagatcgag gcggcgaagg ccgagtggat gcgctacagc gacgtcactg
      421 cgcgccgggt cttcggttcg atcgacggcg agaccgagtt cattccgctg ccggtccgat
      481 agctcgcggg ggcgtcagtt cgagcgcgcg atttccagcg cgcgcagcgc cgcggcggtg
      541 cggttttcga cgccgagctt gcgaaagatg ttctccaggt gcttgtgcac cgtgcgcggc
      601 cggatcgcga gaatcgccgc gatctccgcg ttgctcttgc ccgccgccag ccagtgcagg
      661 atctcgcgct cgcgcgcggt cagcccgtag tcgcgcgccg gcgcgtcggc ggccacgcac
      721 tcgagcatca gcgtcagcgc gtcctcgccc tgcagcaggt gcagcttcag cgtatgcgcg
      781 gcggtggcga gcgcgagcgg gccgggatcg aacatcgccc acgacggctc cagccgcgtg
      841 cgcatccagc ggtcgatcgg ctcgggcagc cgctcgccgg cgcgcacgct tcccgtgaag
      901 ccggcatagg cgagccactt gagcgcgtgt tcggaactgc gtacgacgcg ccgcgtgcgg
      961 tccagcacga tcaccgccat gcccgcaccc atcagcacgt cgttcagttg cgccagggtg
     1021 cggcgcgcgc gcagccgcgt ttcctgcgcc atgtactcgg cgccgagctg gtagcgcagc
     1081 gtgtcgagca gcgcgcaatc gcggtcggag aattcgcgcc gcgcgcgatt gagcacgaag
     1141 ctgatcagcc gcgccgaatc gacgtgcacc ggcaccgcga ccacatagtc gatgccgatg
     1201 cggcggtagt agtcagcgta cagcgcgctg cggcggaagc tcgcatcggg agccgagtcg
     1261 gagatgcgat gggcacctgc gccgaggtgc gtcgagtggt aatgcaccaa gggatggctc
     1321 tggaagaagc ggttgaagca ggcgacgtcc tcgtcggaca gcgattcgtc gggccaggtg
     1381 accacgctgc gcgtgccggc gcgcaggtcg cagtagctca acgtgacgag atcggcgccg
     1441 accagtcctg ccagccccga gatcgccgag cgcgcgaact gctccatcga cggcgcctcg
     1501 atgcgctgcg cgcgcaggaa gtcgagcgcg cgctgcaggt cgccgcccga caacaggctt
     1561 gccgaggatg cggtcatcgc gatcgacccc tgcgccgcat acgccggaat gcgtatgggc
     1621 gcccaagctg acgaggcaaa aactatagct cgtggcgccg gcagtgcgga agccgccgga
     1681 cgaacgcaac gcggaggtga agatggaagt ttcgacaggt tcatggttgc gccgcgcagt
     1741 cgcgccgttg tggtgcgcgg ccgccttggc gggcggcgtc gcgctggcgc aatcgccgct
     1801 cgacggcaag gccttcgacg gcgtgtttct cgagcgcggc aagacgcgcg gcgacgccga
     1861 cacgctgatc ttcagggagg gtcgcttccg ctcgaccgcg tgcgacaagt acggctacgg
     1921 cgacgcgccg taccgcatcg tgtccaccga cggcggcgcg atccgcttcg aggtgaccac
     1981 cgagagcccg aagtacggga agctcgaatg gtcgggggcg gtgcgcggcg acaagctcga
     2041 cgccaccgcg atgatggtgc aacccggcaa ggctccggtc gagaactggg tcgtcgccgg
     2101 cctgaggaaa tgaggagaac gtcgatgaac catgccgaac tgaagcactt cagccagccc
     2161 gacgacgtgc gcgagttccc gcacggccgg ctcgaactga tcaacatcgg cggcgccacg
     2221 gtcggccgcg ccatcttcga gccgggctgg cgctggctga cctcggtgca gccgctggcg
     2281 aacacgaaaa gctgcgaggc gccgcacttc cagtaccacg tggcgggggt gctgatggtg
     2341 aagatggacg acggcaccga gctcgagtgc catccgggcg acgtgtcgct gctgccgtcg
     2401 ggtcacgacg catgggtggt cggcgatgag cccgcggtgg tggtcgactt tcagggcatg
     2461 gtcgactacg cgaaggcgca acacgcgcat tcctgaacgc tgctgactgt catcgagggc
     2521 cgcgcacgac gcggcccttt ctgtccgcgc tcgcgcgcgc taatctgcgc ccattgcgat
     2581 gggtgtggac gatgccgtgg accgagtttg aatatcccgc gtcgatgcgc catctgagcg
     2641 agcccgcgcg cgccaaggcg atcgagatcg ccaacgcgct gctcgacgaa ggcatggacg
     2701 aaggcaaggc gatccggatc gcgatcgcca aggcgaagga gtgggcggta cagcacggtc
     2761 tgccggcgcg cgacgccgag gcgtaacggt ctcatgcgga gccgtcgacc cggccctcgc
     2821 gccgatgtga catcgcgtgc cacggcgcgg tgaaccgacg ggccgggccg caccgccaag
     2881 ttgtcgtcat ggtcaaggag agccagaaca tgaactcacc gatcttgcgg atcccatccc
     2941 ggcgtcgctt caatcaggcc gcggtcgcgg cgaccgcgtg gctcgtgctg ccgggcgcgc
     3001 gcgccgacga ccgtgccgac gcacgcgggg tagtcgacaa ggcgcgcatc accctcgagt
     3061 cgctggtggc ggacaagaac atggacgcgc tgcgcgccgg gctcaggcag gcgcgcggcg
     3121 tgctgatctt tccgcagatc atcaagggcg cattcttcgt cggcggctcc ggcggtaccg
     3181 gtgcgctgct ggtgcgcaat ggcgaaacct ggaccgggcc ggcgttctac acgatcggtt
     3241 cggccagcat cggcgtgcag ttcggcggcg aggcggccga agtcgcgatc ctcgtcaaca
     3301 gccagaaggc gctcgacggc ctgtacggca accggttcaa gctcggcacc gatgcgtcga
     3361 tcgccgccgg gccgatcggg cagggcggcg gcgtgacctt caccagcgac atgacgagct
     3421 tttcgcgctc caagggcgct tttgccggca tcagcctcga gggctcggtg atcgacgtgc
     3481 gcgattcgct gaacaacgcc tactacggcc gcaaggtcac gccggtggag atcctcgcca
     3541 agcgggcggt gtcgaacgct caggccgacg ccttgcgcgc tgcgctccgg gcggcggcga
     3601 agtagcgatc agcgcgcgcc gcgcttacgg taagtgccga tcagctcggc gcgcgcgagc
     3661 acgtggctct gcatcgcctg ctcgagctgc gccttggtcg gccgctgcag gtcgggcagc
     3721 acggtgtcga gcgcgtaca
//
LOCUS       DBNHBY010000653         4284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1399887, whole genome shotgun sequence.
ACCESSION   DBNHBY010000653 DBNHBY010000000
VERSION     DBNHBY010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1399887"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16HB_15600"
     CDS             <1..510
                     /locus_tag="AC16HB_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR5608264.1"
                     /translation="APLFLPRAQALARAGPLAAVSPDPGGVKRAQLFREALEAQLGQP
                     VGSAYLEKRRSAGLVSGSLLAGDVRGATVLLVDDLISTGGTMVRAAETCLAAGATRVY
                     ALAAHGLFNAGASAAIQQPALAGTIVSDSVPPFRLDAAARARVEIVECAPLLAQAIES
                     LHAGFVARG"
     gene            complement(478..1551)
                     /locus_tag="AC16HB_15605"
     CDS             complement(478..1551)
                     /locus_tag="AC16HB_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608265.1"
                     /translation="MTTSAPARPAAPRLEEVGLDEKVAFLRDPRHYPAPPARIDVRET
                     HMSYVFMTAAEAYKLKRPVRYPFLDFSTLAAREASCRAEVALNRRLAPDVYLDVVPLT
                     RAGDGRIALGGAGVAIDWLVRMRRLPAERMLDALIVERRLDARELAPLAERLADFYAA
                     APRAGITPDEDHALLMQEHRQNMDVLRLPRFELERAALAAIDSRLRRFLDENRALLAA
                     RVVAGRVVEGHGDLRPEHVCLTDPPRVIDCLEFNRELRLVDWADEIAFLGLECALLGA
                     PWVGAALHAPIAARLNDEVPPALIHFYTALRACLRARLAVAHLLEPDPRDGARWLPRA
                     RAYLRLASDACAALSARDESRVQ"
     gene            complement(1548..2261)
                     /locus_tag="AC16HB_15610"
     CDS             complement(1548..2261)
                     /locus_tag="AC16HB_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR5608266.1"
                     /translation="MLIASSLRILFEYERGVIFLLGRFWRVKGPGLIIVVPGIQKMQR
                     VDLRIVTMDVPPQDVITRDNVSVKVNAVIYFRVVDPQKAIIQVEKFLIATSQLAQTTL
                     RAVLGKHELDELLSERERLNLDVQRILDAQTDAWGIKVTNVEIKHVDLNETMVRAIAR
                     QAEAERERRAKVIHAEGEKQASATLLEAARMLAQQPEAMQLRYLQTLTAIAGDKSSTI
                     VFPLPGALLDTLLKRGPGA"
     gene            complement(2324..3736)
                     /locus_tag="AC16HB_15615"
     CDS             complement(2324..3736)
                     /locus_tag="AC16HB_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5608267.1"
                     /translation="MVRIDWIRSTRAIAAVALIACGLAFAAVSDAPRVLQVEIDGIIG
                     PQTADLVKRSIDRAAAGKMQAVILRMDTPGGLDTSMREIIKAILAAPLPVVTYVAPEG
                     ARAASAGTYILYASHVAAMAPATNLGAATPVAIGIGGEKPAPRERQPEESKPAADKAK
                     PGKTAKGEAKRDESMPADAMTAKSVNDAAAYIRGLAELRGRNADFAEQAVRRASSLAA
                     HEALKQRVIDLMAPDVGTLLKSLDGRTVAIGSGKVMLQTADARVETVVPDWRYKLLAL
                     LSNPTVAVLLMMIGIYGLLYEFTSPGFVAPGVAGAICLLLALYAFHLMPVNWAGVGLL
                     ALGMGLLIAEAFVGGFGVLGVGGIVALVMGGVLLLDIELPGYGVPLWFIFGMAASSAL
                     VVFGIAALVARARRRPVVAGREELIGADGLVVESNADETWARVHGEIWRVRAEAPLAP
                     GARVRVQSLDGLTLVVRPDN"
     gene            3908..>4284
                     /locus_tag="AC16HB_15620"
     CDS             3908..>4284
                     /locus_tag="AC16HB_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5608268.1"
                     /translation="MLELTPAITTPAREPDVEGVSRRLLALIEALLAQMRGAPVTARL
                     EARLDADLGVDSLARVELGLRIEREFGVRLREDALLAADTPRDLLAAILAAGVAVTPA
                     QAGAPALPALEGTPEGARTLLDVL"
BASE COUNT          601 a         1527 c         1537 g          619 t
ORIGIN      
        1 gcgccgctct tcctgccgcg cgcgcaggcg ctggcgcgcg ccgggccgct cgcggccgtg
       61 tcgcccgacc ccggcggcgt caagcgcgcg cagctgttcc gcgaagcgct ggaggcgcag
      121 ctcggccagc ccgtcggcag cgcgtatctc gagaagcggc gcagcgccgg cctggtgtcg
      181 ggctcgctgc tcgcgggcga tgtgcgcggc gcgaccgtgc tgctggtcga cgacctgatc
      241 agcaccggcg gcacgatggt gcgcgcggcg gagacgtgcc ttgcggccgg cgcgacgcgc
      301 gtctacgcgc tcgccgcaca tgggctgttc aacgcggggg ccagtgcggc gatccagcaa
      361 cccgcgctgg ccggcacgat cgtcagcgac agtgtgccgc cgttccgcct cgacgccgcg
      421 gcacgcgcgc gcgtcgagat cgtcgaatgc gcgccgctgc tggcgcaggc gatcgaatca
      481 ctgcacgcgg gattcgtcgc gcgcggatag cgccgcgcac gcgtcgctcg ctagccgcag
      541 ataggcgcgt gcgcgcggca gccagcgcgc accgtcacgc ggatcgggtt cgagcagatg
      601 ggcaacggcg agccgcgcgc gcaggcaggc gcgcagcgcg gtgtaaaaat ggatcagcgc
      661 gggcggcacc tcgtcgttca gccgggcggc gatcggcgcg tgcagcgcgg cgccgaccca
      721 cggtgcgccg agcagcgcgc attcgaggcc gaggaacgcg atctcgtcgg cccagtccac
      781 cagccgcagc tcgcggttga actcgaggca gtcgatcacg cgcggcggat cggtcaggca
      841 cacgtgctcg ggccgcaggt cgccgtgacc ctcgaccacg cggccggcga ccacgcgcgc
      901 ggccagcagc gcgcggtttt cgtccagaaa ccggcgcagt cgcgaatcga tcgccgcgag
      961 cgcggcgcgc tcgagttcga agcgcggcag ccgcagcacg tccatgttct gccgatgctc
     1021 ctgcatcagc agcgcatggt cttcgtcggg cgtgatgccg gcgcgcggcg cggccgcgta
     1081 gaaatccgcc agccgttccg cgagcggcgc gagttcccgc gcgtcgagcc ggcgctcgac
     1141 gatcagagca tcgagcatcc gctcggccgg cagacgccgc atccgcacca gccagtcgat
     1201 cgcgacgccg gcgccgccga gcgcaatgcg tccgtcgccc gcacgcgtca gcggcacgac
     1261 gtcgaggtag acatcgggcg ccagccggcg gttcagcgcg acctcggcgc ggcagctcgc
     1321 ctcgcgcgcg gcgagcgtgc tgaagtcgag gaacggatag cgcaccgggc gcttgagctt
     1381 gtacgcctcg gcggcggtca tgaacacgta cgacatgtgc gtctcgcgca cgtcgatgcg
     1441 cgccggcggg gccgggtagt ggcgcgggtc gcgcaggaac gcgacctttt catccagccc
     1501 gacctcttcc agccgcggcg cggcagggcg cgccggcgcg ctcgtggtca tgcgcccggg
     1561 ccgcgtttga gcagtgtatc gagcagcgca cccggcagcg ggaacacgat ggtcgagctc
     1621 ttgtcgccgg cgatcgcggt cagcgtctgc aggtagcgca gctgcatcgc ctcgggctgt
     1681 tgcgccagca tgcgcgcggc ttcgagcagc gtggcggagg cctgcttctc gccctcggcg
     1741 tggattacct tggcgcgccg ctcgcgttcg gcctcggcct ggcgcgcgat cgcgcgcacc
     1801 atcgtctcgt tcagatccac gtgcttgatc tcgacgttgg tgaccttgat gccccaggcg
     1861 tcggtctgcg cgtcgaggat gcgctgcacg tcgagattga gccgctcgcg ctcggacagc
     1921 agctcgtcga gctcgtgctt gccgaggacc gcgcgcagcg tcgtctgcgc gagctggctg
     1981 gtggcgatca ggaacttctc gacctgaatg atcgccttct gcggatcgac cacgcggaaa
     2041 tagatcaccg cgttgacctt gaccgacacg ttgtcgcgcg tgatcacgtc ctgcggcggc
     2101 acgtccatgg tgacgatgcg caggtcgacg cgctgcatct tctggatgcc gggcacgacg
     2161 atgatcagcc ccggcccctt gacgcgccag aagcggccga gcaggaagat gacgccgcgc
     2221 tcgtattcga acaggatgcg cagcgaggag gcgatcagca tcgcgagcag gatgacgacg
     2281 gtcgcgagtc cgaacggcag gttggcgatc atggcgctcc tcttcagttg tccggccgca
     2341 cgacgagcgt cagcccgtcg agggactgca cgcgcacgcg cgccccgggg gcgagcggcg
     2401 cttcggcgcg cacgcgccag atctcgccgt gtacgcgcgc ccaggtctcg tcggcgttcg
     2461 actcgaccac gaggccgtcg gcgccgatca gttcctcgcg cccggccacg accggccgcc
     2521 ggcgcgcacg cgcgaccagc gccgcaatgc cgaacaccac cagcgcgctg gacgcggcca
     2581 tgccgaagat gaaccacagt ggcacgccat agcccggcag ttcgatgtcg agcagcagca
     2641 cgccgcccat caccagcgcg acgatgccgc cgaccccgag cacgccgaag ccgccgacga
     2701 acgcctccgc gatcagcagt cccatgccga gcgccagcag gccgacaccg gcccagttca
     2761 ccggcatcag gtggaacgcg tacagcgcga gcagcaggca gatcgcgccc gccacgcccg
     2821 gcgcgacgaa tccggggctg gtgaactcgt acagcaggcc gtagatgccg atcatcatca
     2881 gcagcaccgc cacggtcggg ttcgacagca acgccaacaa tttgtagcgc cagtcgggaa
     2941 cgacggtctc gacccgggcg tcggcggtct gcagcatcac cttgccgctg ccgatggcga
     3001 ccgtgcggcc gtcgagcgat ttgagcagtg tgccgacgtc gggcgccatc aggtcgatca
     3061 cgcgctgctt cagcgcctcg tgcgccgcga ggctgctggc gcggcgcacc gcctgctcgg
     3121 cgaagtcggc gttgcggcca cgcagttcgg ccagcccgcg gatgtaggcc gcggcgtcgt
     3181 tgaccgactt ggccgtcatc gcgtcggcgg gcatcgattc gtcgcgcttg gcctcgccct
     3241 tggcggtctt gccgggcttt gccttgtccg cggcgggctt gctctcttcg ggttgccttt
     3301 cgcggggcgc gggtttctcg ccgccgatgc cgatcgccac tggcgtcgca gcgccgaggt
     3361 tggtcgccgg cgccatcgcg gcgacgtggc tcgcgtacag gatgtacgtg ccggcgctcg
     3421 cggcgcgcgc cccctccggc gcgacgtagg tgaccaccgg cagcggcgcc gcgaggatcg
     3481 ccttgatgat ctcgcgcatc gaggtgtcga gcccgcccgg cgtgtccatg cgcaggatca
     3541 ccgcctgcat cttgccggcc gccgcgcgat cgatcgagcg cttgaccagg tctgccgtct
     3601 gcgggccgat gatgccgtcg atctcgacct gcagcacgcg cggcgcgtcg ctgaccgccg
     3661 cgaaggccag cccgcacgcg atcagcgcaa ccgcggcgat cgcgcgcgtc gatcgtatcc
     3721 agtcgatccg aaccatggtc ggaactcgtc caagcgcccg gccgccgccg caatcacgtc
     3781 cgcgtatccc aatgacacgt tacggggcgt cgggttccga gcattgacgg ctatcagtgt
     3841 ggcgcacacg gtttcgtgcg gcaatctttg gaagtggatg ggcagcacgg tgccatcccg
     3901 cctttaaatg ctcgaactca ccccggcgat cacgacgccc gcgcgcgagc cggacgtcga
     3961 gggtgtgtcg cgtcgcctgc tggcgctgat cgaggccctg ctggcgcaga tgcgcggcgc
     4021 gccggtgacg gcgcggctgg aggcgcggct cgatgccgat ctcggcgtcg acagcctggc
     4081 gcgcgtggag ctcggcctgc ggatcgagcg cgagttcggc gtgcgcctgc gcgaggacgc
     4141 gctgctggcc gccgacacgc cgcgcgacct gctcgcggcg atcctcgcgg cgggtgtggc
     4201 ggtgacgccg gctcaggccg gagcgcccgc gctgccggcg ctcgagggaa cgccggaggg
     4261 cgcgcgcacg ctgctcgacg tgct
//
LOCUS       DBNHBY010000654         5081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1805259, whole genome shotgun sequence.
ACCESSION   DBNHBY010000654 DBNHBY010000000
VERSION     DBNHBY010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1805259"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1456
                     /locus_tag="AC16HB_15625"
     CDS             176..1456
                     /locus_tag="AC16HB_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5608269.1"
                     /translation="MRFIAKLAIAAGAALLMHAGAMAQGKGVPAEQTVKLAFVDPLSG
                     LMGPVGSNILRSYQFVVEMANKEQWAKGYKFEFVPFDNKLSPQESLNALKLAIDQDIR
                     YIVQGNGSSVALALTDAVAKHNERNPGKEVLFLNYAAVDPDLTNTKCSFWHFRFDANS
                     DMKMEALTSYMAKQKDIKNVYLINQDYAFGHQVRRAAREYLARKRPDVKIVGDDLHPL
                     ARVTDFSPYVAKMKAANADTIITGNWGSDLSLLIKAAKDAGLNANFFTYYATTTGVPT
                     AMGSSGVGRVHYVGYWNLNGDPYDKAGLGEKMFLDFKKRFNEDYYTLATYNVLRTLAA
                     AISKAKSADPVKVAFAMEGLKFESINGEVEMRAADHQLQQPLWITSWQKAGTQGVKFD
                     SENTGYGWRTEELLPPYVAAQPTSCQMKRPGKPG"
     gene            1506..2459
                     /locus_tag="AC16HB_15630"
     CDS             1506..2459
                     /locus_tag="AC16HB_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5608270.1"
                     /translation="MTVELALISLLNGISYGLLLFMLASGLTLIFSMMGVLNFAHASF
                     YMLGAYFGYQISQWIGFWPGLLIAPIAVGVLGAAVERWGLRRVHKWGHVPELLFTFGL
                     SFIVVELVQVVWGRPPVPNELPRELQGSLFTIFSTNFPLSRGFIMLVAVLMLAALWLV
                     VTRSRIGLVIQAALAKPQQVEALGHNVPRVFMLVFGAGSALAGLAGVIGGAVYVTEPA
                     MASTAGAIIFVVIVVGGMGSLSGAFLASLLIGLIDSFAKVIDNSLLDGLAALGVSISP
                     QTPLYSVWTLKLSQVAPVLPFLLLVLVLIVRPKGLLGTRED"
     gene            2459..3802
                     /locus_tag="AC16HB_15635"
     CDS             2459..3802
                     /locus_tag="AC16HB_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5608271.1"
                     /translation="MEAGSQRATSPPNFTGPNLRYRPLNLGRWVVWGLTVLLFACMPL
                     VFHSGYARTLLSQMGILIIFALSYNMLLGQGGMLSFGHAVYSGLGAYFAIHAMNLIGA
                     GQLSLPVSLLPLVGGFAGLFFGVVFGYVTTKKAGTPFAMITLGLGELVFSCSLMFPAF
                     FGGEGGISGNRTAGGPLFGVAGWTLASQIQVYYLIALWCLFCMAAMFAYTHTPLGRIA
                     NAVRDNPERAEFIGYNTQRVRWFVLILSAFFAGIAGGLSAINFEIVTAENVSAVRSGG
                     VLLAAFIGGVAFFFGPILGAVVFTLFAVALSEFTKAWQLYLGVFFVLLVMFAPGGLSS
                     LILMNLRVLKFRKFKRLADPYVGVILSALALTLGCVMLVEMIYHRTLDFALGPRIHLF
                     GFPVYTDRAESWVFAMTVLIAGAAAFEYARRQFKATWDTIQLEIEDWIRDNPEDA"
     gene            3799..4557
                     /locus_tag="AC16HB_15640"
     CDS             3799..4557
                     /locus_tag="AC16HB_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608272.1"
                     /translation="MISAALELVDVRKSFGRTEIIRSVNLAVPAGERHAIIGPNGAGK
                     STLFNLVSGRFPVSSGTIRLNGADITGRKPFQINRLGLSRSFQITNIFHRLSVFENLR
                     CAVLWSLGYRYSFWHNLNKLGDAEDRAEAILNRIGLRRRRNIAAGLLTYAEQRALEIG
                     ITIAGDASVILLDEPTAGMSRSETAHAVALIRDVTQGKTLLIVEHDMSVVFGLADRIS
                     VVVYGEVIATGAPAEIRANAKVKEAYLGTQAEAH"
     gene            4557..>5081
                     /locus_tag="AC16HB_15645"
     CDS             4557..>5081
                     /locus_tag="AC16HB_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608273.1"
                     /translation="MLEVRNLQAFYGKSHVLHGVDLDVGQGEIVSLLGRNGVGRSTTA
                     KAIMGMVDCTGTVRFKGRDLTRLKTYQIAHAGIGYVPESRDIFPTLTVEENLKLGLKG
                     ARPGRWSYADCYRLFPRLQERARTQAGVLSGGEQQMLTLARTMMGDPELAIIDEPTEG
                     LAPMLVELVAKFLRE"
BASE COUNT          864 a         1604 c         1669 g          944 t
ORIGIN      
        1 tcgtcgacag cattccgctc ggcgccaccg gcaagatgct gaagaaccgc ctgcgcgagc
       61 agttcaagga ttacaggctg ccgagcgcgt gaagcgcttt acgctgcaag acgcgttgag
      121 tattctcccc cggcatagag tcgacacccc acccgagacg acaggagacg ccatcatgcg
      181 gttcattgcg aaactcgcca tcgccgcggg cgcggcactt ctgatgcatg ccggcgcaat
      241 ggcgcagggc aagggcgtac cggccgagca gacggtgaag ctcgctttcg tcgacccgct
      301 gtccggtctc atggggccgg tcggcagcaa catcctgcgc agctaccagt tcgtggtcga
      361 gatggcgaac aaggagcagt gggccaaggg ctacaagttc gagtttgtcc cgttcgacaa
      421 caagctcagt ccgcaggaaa gcctgaacgc gctcaagctg gcaatcgacc aggatatccg
      481 gtacatcgtg cagggtaatg gctcgagcgt ggcgctggcg ctcaccgatg cggtcgccaa
      541 acacaacgag cgcaacccgg gcaaggaggt tctgttcctc aactacgccg cggtcgaccc
      601 cgacctgacc aacaccaagt gcagcttctg gcacttccgc ttcgacgcca attccgacat
      661 gaagatggag gcgctgacgt cgtacatggc caagcagaaa gacatcaaga acgtctacct
      721 gatcaaccag gactacgcat tcggccacca ggtccggcgc gcggcgcgcg aatacctggc
      781 gcgcaagcgg cccgacgtca agatcgtcgg cgacgacctg cacccgctcg cgcgcgtcac
      841 ggatttttcg ccgtacgtgg cgaagatgaa ggccgcgaac gcggacacca tcatcaccgg
      901 caactggggc tccgacctgt cgctgctgat caaggcggcg aaggacgccg gcctgaacgc
      961 caatttcttc acgtactacg ccaccacgac tggcgtgccc accgcgatgg gctcgagcgg
     1021 cgtcggacgc gttcactatg tcgggtactg gaatctgaac ggcgacccgt acgacaaggc
     1081 cggcctgggc gagaagatgt tcctcgactt caagaagcgt ttcaacgagg actactacac
     1141 gctggccact tacaacgtgc tgcgcacgct ggctgcggcc atcagcaagg cgaagtcggc
     1201 cgatccggtg aaagtggcgt tcgcgatgga agggctgaag ttcgagagca tcaacggcga
     1261 ggtcgagatg cgcgcggccg accatcaatt gcagcagccg ctgtggatca cgagctggca
     1321 gaaggccggg acccaggggg tgaagttcga ctcggagaac accggttacg gatggcgcac
     1381 cgaagagctg ctacccccat acgtcgcggc ccagccgacg agctgccaga tgaagcgtcc
     1441 gggcaagccg ggctagaaga cgcgcagaag cggcgaccgg cctccgtggc cggtcttttt
     1501 tttccgtgac cgtcgaactc gcgctgatct cgctgctgaa tggcatcagc tacgggctgc
     1561 tgctgttcat gctggcgtcg gggctgacgc tgatcttctc gatgatgggc gtgttgaatt
     1621 tcgcccacgc cagcttctac atgctgggcg catatttcgg ttaccagatc tcgcagtgga
     1681 tcgggttctg gcccgggctc ttgatcgcac cgatcgccgt tggagtgctg ggtgccgcgg
     1741 tcgagcgctg ggggctgcgc cgcgtgcaca agtggggaca cgtgcccgag ctgctgttca
     1801 ccttcgggct gtctttcatc gtcgtcgaac tcgtccaggt ggtctggggc agaccgccag
     1861 tcccgaatga attgccgcgg gaactgcagg gctcgctgtt cacgatcttc tccaccaact
     1921 ttccgttgtc gcgcgggttc atcatgctgg tggcggtgct gatgctcgct gcgctctggc
     1981 tggtcgtcac gcggtcgcgc atcgggctcg tcatccaggc agcgctggcg aaaccgcagc
     2041 aggtcgaagc gctcggccac aacgtgccgc gcgtgttcat gctggtgttc ggtgccggct
     2101 ccgccctggc cggactggcg ggggtgatcg gcggcgccgt ttacgtgacg gagccggcga
     2161 tggcgtcgac ggcaggcgcg atcatcttcg tggtgatcgt cgtcggcggc atgggatcgc
     2221 tgtcgggcgc gtttctcgcg tcgctgctga tcggcctgat cgattccttc gccaaggtca
     2281 tcgacaactc gctgctcgat ggcctggcgg cgctcggcgt atcgatctcg ccgcagacgc
     2341 ccttgtacag cgtgtggacg ctgaagctgt cgcaggtcgc gccggtgctg cctttcctgt
     2401 tgctggtgct ggtcctgatc gtgcggccga agggcctgct cggcacgcgc gaggactgat
     2461 ggaagccgga tcccagcgcg cgacatcgcc gccgaacttc acgggtccga acctgaggta
     2521 ccggccgctc aacctggggc gctgggtcgt ctgggggctg accgtactgc tgttcgcctg
     2581 catgccgctc gtcttccact cgggatatgc acgcacgctg ctgtcgcaga tgggcatcct
     2641 gatcatcttc gcgctctcgt acaacatgct gctcggacaa gggggcatgc tgagcttcgg
     2701 ccacgcggtc tactcgggtt tgggtgcgta cttcgccatc catgcaatga acctcatcgg
     2761 cgcgggccaa ctgagtcttc cggtgtcgct gctgccgctg gtcggcggat tcgccggctt
     2821 gttcttcggg gtcgtattcg ggtacgtcac gacaaagaag gcgggcaccc cgttcgcgat
     2881 gatcacgctc gggctgggag aactggtctt ctcttgctcg ctgatgttcc ccgcgttctt
     2941 cggcggcgaa ggcggtatca gcggcaaccg aaccgcgggc ggtccgctgt tcggcgtcgc
     3001 aggctggacg ctggcctcgc agatccaggt ctattacctg atcgcgctct ggtgcctctt
     3061 ctgcatggcg gcgatgttcg cctacacgca cacgccgctg ggacgcattg ccaacgcggt
     3121 gcgcgacaac ccggagcgcg ccgagttcat cggctacaac acgcaacgag tgcggtggtt
     3181 cgtgctcatc ctgtcggcct ttttcgccgg catcgccggc gggctgtcgg cgatcaactt
     3241 cgagatcgtc accgcggaga acgtcagtgc ggtccgttcc ggcggcgtgc tgctggccgc
     3301 attcatcggc ggagttgcct tcttcttcgg gcccatactc ggcgccgtcg tcttcaccct
     3361 ttttgcggtc gcgctgtcgg agttcaccaa ggcctggcag ctctacctcg gcgtgttctt
     3421 cgtgctgctg gtgatgttcg cgcccggcgg gctgtccagc ctgatcctga tgaatttgcg
     3481 cgtgctgaag ttccgcaagt tcaagcggct cgccgatccg tatgtcggag tgatcctgtc
     3541 ggcgcttgcg ctcacgctcg gatgcgtgat gctggtcgag atgatctatc accggacgct
     3601 cgactttgcc ctcggcccgc ggatccatct cttcggcttt ccggtctaca cggatcgcgc
     3661 cgaaagctgg gtgttcgcaa tgacggtgtt gatcgccggc gccgccgctt tcgagtatgc
     3721 gcgccggcaa ttcaaggcga catgggacac gatccagctc gagatcgagg actggatccg
     3781 cgacaacccg gaggacgcat gatttctgcg gcgctcgaac tggtcgacgt ccgcaagagc
     3841 ttcggccgca ccgagatcat ccgcagcgtg aacctcgccg tgccggcggg cgagcggcac
     3901 gcgatcatcg ggccgaacgg tgccggcaaa tcgaccctgt tcaacctggt cagcggccgc
     3961 ttcccggtca gcagcggaac gatccggctc aacggcgccg acatcaccgg ccgcaagccg
     4021 ttccagatca accgcctcgg cctgtcgcgc agcttccaga tcaccaacat cttccatcgg
     4081 ctgtcggtgt tcgagaacct gcgctgcgcg gtgctgtggt cgctcggtta ccgctattcg
     4141 ttctggcaca acctgaacaa gctcggcgat gccgaggacc gcgccgaggc gatcctgaat
     4201 cgaatcgggc tgcgccgccg gcgcaacatc gcggcgggac tgctgaccta cgccgagcag
     4261 cgtgcgctcg agatcggcat cacgattgcc ggcgacgcca gcgtgatcct gctcgacgag
     4321 ccgacggcgg gcatgagccg cagcgaaacc gcccacgccg tggcgctgat ccgcgatgtg
     4381 acacagggca agacgctgct aatcgtggag cacgacatga gcgtcgtctt cggactcgcc
     4441 gaccgcatct cggtggtcgt gtacggcgag gtcatcgcca cgggtgcgcc ggcggaaata
     4501 cgcgccaatg cgaaggtgaa ggaggcctac ctcggcacgc aggcagaggc acactgatgc
     4561 tggaagtccg caacctgcag gcgttctacg gcaagagcca cgtgctgcac ggcgtggatc
     4621 tggacgtcgg gcagggcgaa atcgtcagcc tgctcgggcg caacggcgtc gggcgctcga
     4681 ccacggccaa ggcgatcatg ggcatggtcg actgcacggg caccgtgcgc ttcaaggggc
     4741 gcgacctgac acggctgaag acctaccaga tcgcgcacgc tggcatcggc tacgttccgg
     4801 agagccgcga tatcttcccg acactgacgg tcgaggagaa cctgaagctc gggctgaagg
     4861 gagcgcggcc gggacgctgg agctacgcgg actgctatcg gctgtttccc cgcctgcagg
     4921 agcgggcacg gacgcaggcc ggcgtgctgt cgggcggcga gcagcagatg ctgacgctgg
     4981 cgcgcacgat gatgggcgat ccggaactgg cgatcatcga cgagccgacc gagggcctgg
     5041 cgccgatgct cgtcgagttg gtcgcgaaat tcctgcgtga g
//
LOCUS       DBNHBY010000655         5633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_385260, whole genome shotgun sequence.
ACCESSION   DBNHBY010000655 DBNHBY010000000
VERSION     DBNHBY010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_385260"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1072
                     /locus_tag="AC16HB_15650"
     CDS             242..1072
                     /locus_tag="AC16HB_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR5608274.1"
                     /translation="MQCAARWISAGLSERGTLALIAAVVAIESARMLAAPTADGAVAA
                     VVLLAALVSSIAGFAFAAIAGSALAYLRFEPVQAVLTMALCSIAIQAYAVWQLRASIR
                     WRALAPMLAAGALTTPLGVALLIHIDARLYAAVLGAIVTGYGSFVLLRRPTRVVRGTV
                     WHDALAGALGGVVGGLSSAPGLAVTVWCSMRGWDKVQQRAVYQPFILAMQFVTVACLA
                     WSAPTQADPLRDASFVPFALLGAIGGFALFRRLTTQQFQAAVSLLLIVSGVGLLARAY
                     "
     gene            1138..1512
                     /locus_tag="AC16HB_15655"
     CDS             1138..1512
                     /locus_tag="AC16HB_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5608275.1"
                     /translation="MPKRQARGPYAVILASGPEDGGKRATLALAAACTAQAMDLDTIV
                     FLIGDGAHWAYDGHAEQIHAPGFPALGDLIDSFVESGGQLLLCSACDAICARPAARRQ
                     DVRVKGLASLLSHTASGSSVTF"
     gene            1648..3336
                     /locus_tag="AC16HB_15660"
     CDS             1648..3336
                     /locus_tag="AC16HB_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030256 - type I protein secretion system
                     complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HIR5608276.1"
                     /translation="MKPILARQMHLLLAAAAFSLCVNLLALAGPLYMLQVYDRVLASR
                     SLETLVLLGLIALAALAAFAWLDSIRLQLLTRVAVRIEAAAAAPILRRTLATAGIGAR
                     PYRHGLADLRTMRSFVSGAGCLALTDAPWCPVFVGLICLFHPLMGVVTVVGVALLLAL
                     AWLDDRLSKGAVEAAVLSSRKVADMADEASLNGEVVRALGMQASIEERLGAASERAAD
                     RFLAAGRASSRVLASSKLLRQSLQIVMLGLGAYLVIRQQVSPGAMIAATLLLSRAMAP
                     IESAISGWRLMIEARAAYRRVAACLDVESPPAPMRLPLPQGELAVERATLAPDPKSPI
                     LKGVSFTLDRGDLLGLIGPSGAGKSMLGRLLVGVLRPTLGNVRLDGADLHQWHADTLG
                     THIGYLPQDVQLFDGTVAENIARLRDPDAEHERIVRAARIAGVHELILRLPNGYDTQI
                     GSRGCALSSGQRQLLGLARAVFGEPALIVLDEPNANLDAEGEDRLIAALRCLKERRAT
                     VVMITHRPALLRDADKVLVLRGGAVAEFGERESVLGRLARVTAMPVPTKRAAVG"
     gene            3353..4675
                     /locus_tag="AC16HB_15665"
     CDS             3353..4675
                     /locus_tag="AC16HB_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608277.1"
                     /translation="MTMTLSANDGRYAGDRHLIGTGIAIVLAVFGVFSTWAALAPLHG
                     AVIVAGTVVVESERKTVQHLEGGIVKRILVKPGSIVAQGEPLIQLEEVAADAAVSAIR
                     AQLDADVARVARLVAERAAAAKIDFPRELMQRAAQPGVASILDTERALFVTRRKVLEE
                     QIALLRAENTHIREEITNLDLQVKAGDAGIGYAREQLDLNEQLLRENFVANARVLDFR
                     GQLSDRQQKRAEAAAQAAQARQKIKQNELKIEGLRQTYVKDATDELRQAERRVDDLRE
                     RLRPTQDALSRSTIVAPIAGVVVDLKVHTVGGVIGPREALMDIVPAEAPMIVKGKARA
                     EDITHLRVGTDVAVQFTAYKRRTTPVVNGAITYVSADALAEPSPLGTTPYYEVHISVP
                     RAALKEAGDLDIVPGMPVEAFVQTEARTVLDYLIQPLTQSLRRAGREY"
     gene            4759..>5633
                     /locus_tag="AC16HB_15670"
     CDS             4759..>5633
                     /locus_tag="AC16HB_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR5608278.1"
                     /translation="MSTIFGTTATETLNGTAGDDTIYGGPTVDPNATNTGRDTINSGS
                     GNDIVYGQDGDDYLYGQDGGDTLYGGTGYDALYGGPGNDVLDGGADGDYLTGGNTMSE
                     STVEIGTGDDRLNGGAGNDTLLGHDGNDVLNGGDGNDRLVGHTGFNVLNGEAGDDTLI
                     SAGVNDMLDGGTFGESATLGDLAILDRSTATSALSLALDSQVGNAQQNLGDGTLITNI
                     ERLDFTGGAGDDVISGLTAGDYSDVLRGGAGADTLSGGGGIDQLYGGTGNDMLDGGAG
                     RDNLYGGDTQSSTET"
BASE COUNT          895 a         1946 c         1964 g          828 t
ORIGIN      
        1 gcagcgtggc cggccacgcc agaacaactc caacgctgga gcgtgatcgt cgcggccgat
       61 ctgcgcgagc acggtgcgcc ccgcaccgag cacgacggcg agtgacgccg caacgaacgc
      121 cgccgaacgc tcgcatcgac cacggcaccg gcgcatcgga cctgtcatga agaccatcga
      181 ctcacccacg ggcctcggat cgagcccgta cccgacatcg gaaagtcaaa tggaacgtgc
      241 aatgcaatgc gcagcgcgct ggatcagcgc cggtttgagc gagcgcggca cgctggcgct
      301 gatcgcggcc gtggtcgcca tcgagtcggc gcggatgctt gccgcgccga ccgccgacgg
      361 cgcggttgcg gcggtcgttc tgctggcggc gctggtatcg agcatcgccg gcttcgcgtt
      421 cgccgccatc gccggcagcg cacttgccta cctgcggttc gaacccgtcc aggcggtgct
      481 gacgatggcg ctgtgctcga ttgccatcca ggcctatgca gtctggcaat tgcgcgcatc
      541 gatccgctgg cgcgcactgg cgcccatgct ggccgcaggc gccctgacga cgccgctcgg
      601 ggtcgcactg ctgattcaca tcgacgcacg gctgtatgcc gcagtgctcg gcgccatcgt
      661 gaccggatac ggcagcttcg tgctgctgcg tcggccgacc cgggtggtcc gcggcacggt
      721 gtggcacgac gccctggccg gcgcgctcgg tggtgtggtg ggcggcttgt cgagcgcgcc
      781 ggggctcgcc gtgacggtct ggtgctcgat gcgcggctgg gacaaggtgc agcagcgcgc
      841 cgtgtatcaa cccttcatcc tggcgatgca gttcgtcacc gtcgcgtgcc tggcgtggag
      901 cgcaccgacg caggccgatc cgttgcgcga cgcgagcttc gtcccgtttg cgctgctggg
      961 tgcgatcggt ggattcgccc tgttccgccg cctgaccaca cagcagttcc aggcggcagt
     1021 cagcctgctt ttgatcgtct cgggcgtcgg cctgctggcg cgcgcgtact gacagcaaaa
     1081 cgaaaggagt gccccatgca agtgaattcg cttcccacgt caactttgtc gcgaccgatg
     1141 ccgaagcgtc aggcacgcgg cccgtatgca gtgatcctcg ccagcggtcc ggaagacggc
     1201 ggcaagcgcg ccacgctcgc gctcgccgcg gcctgcaccg cacaggcgat ggatctggac
     1261 acgatcgtgt tcctcatcgg cgacggcgcg cactgggcgt acgacggaca tgccgagcag
     1321 atacacgcgc ccggctttcc ggcactcggg gacctgatcg attcgttcgt cgaatccggc
     1381 gggcaactgc tgctgtgctc cgcgtgcgat gccatctgcg cacggcccgc cgcgcggcgc
     1441 caggacgtgc gcgtcaaggg gctcgcctcg ctgctgtccc acaccgccag cggaagttcc
     1501 gtgacgttct gaaccccagg gctggcattc accacaggag acgcagctca tgaggaccat
     1561 ccgcggtacg acgggcgcca tgcgccctgt gcaaagcacc gccgagggcg gtagccccgc
     1621 cgcagtcgtc aggcacgggg gccagtcgtg aagccgatcc tcgcgcgaca gatgcacctg
     1681 ctgttggcgg cggccgcgtt cagcctgtgc gtcaacctgc tcgcgctcgc cgggccgctg
     1741 tacatgctgc aggtgtacga ccgcgtcctc gccagccgca gcctggagac gctcgtgctg
     1801 ctcggactga tcgcgctggc ggcactcgcg gccttcgcct ggctcgattc catccgcctg
     1861 cagctgctga ctcgcgtggc ggttcggatc gaggcggccg cggccgcgcc gatcctgcgc
     1921 cgcacgctcg cgactgccgg catcggcgcg cgaccctatc ggcacggact ggccgatctg
     1981 cgcacgatgc gcagtttcgt gtccggcgca ggatgcctcg cgctgacgga cgcgccgtgg
     2041 tgcccggtct tcgtcgggct catctgcctg tttcatccgc tgatgggcgt cgtcacggtc
     2101 gtgggcgtcg cgctgctgct cgcgctcgcc tggctcgatg accggttgag caagggtgcc
     2161 gtggaggccg ccgtgctgtc ttcgcgcaag gtcgccgaca tggccgacga ggcgtcgctc
     2221 aatggcgaag tcgtgcgcgc gttaggcatg caggcgtcga tcgaggagcg gctcggcgca
     2281 gcgagcgagc gcgcagcgga ccgcttcctc gccgccggcc gggcgtcgag tcgcgtcctg
     2341 gcgtcttcca agctgctgcg gcagtcgctg cagatcgtga tgctcggact gggcgcctat
     2401 ctggtgattc gtcaacaggt gtcgccgggc gcgatgatcg cggccacgct tctgctcagc
     2461 cgcgcgatgg cgccgatcga gtcggcgatc tcgggctggc ggctgatgat cgaggcgcgc
     2521 gccgcctacc ggcgtgtcgc cgcttgtctc gacgtcgaaa gcccccccgc gccgatgcgg
     2581 ttgccgctgc cgcagggtga gctcgcagtc gagcgcgcga cgctggcgcc ggatccgaag
     2641 tcgcccatcc tcaaaggcgt gtcattcacg ctcgaccgcg gcgacctgct gggcctgatc
     2701 ggtccgagcg gcgccggcaa gtcgatgctc ggacgcctgc tggtcggcgt gctgcggccc
     2761 acgctcggca acgtgcggct cgacggcgcc gacctgcacc agtggcacgc cgacacgctc
     2821 ggcacgcaca tcggttacct gccgcaggat gtacagctgt tcgacggcac cgtcgccgag
     2881 aacatcgctc gcctgcgcga tccggacgcc gaacacgagc ggatcgtgcg cgccgcgcgg
     2941 atcgccggcg tgcacgaact gatcctgcgc ctgcccaacg gttacgacac gcagatcggc
     3001 tcacgcggct gcgcgctgtc gtctgggcaa cggcagttgc tgggcctggc ccgcgccgtc
     3061 ttcggcgaac cggcgctgat cgtgctcgac gagccgaacg ccaatctcga cgcggagggt
     3121 gaggacaggc tgatcgcggc actgcgctgc ctgaaggaac ggcgcgcgac cgtcgtgatg
     3181 atcacgcacc ggccggcgct gctgcgtgac gcggacaagg tgctcgtgct gcgcggaggc
     3241 gcggtcgccg agttcggcga gcgcgagtcc gtcctcggac gccttgcgcg cgtcacggcg
     3301 atgcccgtgc cgaccaaacg cgctgccgtg gggtgatgcc atgcctgacc caatgaccat
     3361 gacactttcc gccaacgacg ggcgttatgc cggcgaccgg cacctgatcg ggaccggcat
     3421 cgcgatcgtc ctcgcggtct tcggcgtgtt cagcacctgg gcggcgctgg caccgctgca
     3481 cggcgcagtg atcgtcgccg gcaccgtggt ggtcgagtcc gaacgcaaga cggtgcagca
     3541 tctcgagggc ggcatcgtca agcgcatcct ggtcaagccg ggctcgatcg tcgcgcaggg
     3601 cgaacccctg atccagctcg aggaagtggc tgccgatgcc gcggtcagcg cgatccgggc
     3661 gcagctcgac gcggacgtgg cgcgcgtggc gcggctcgtg gccgagcgcg cggccgccgc
     3721 gaagatcgat ttcccgcgcg aactgatgca acgcgcggcc cagcccggcg tggcatcgat
     3781 tctcgatacg gagcgggcgc tgttcgtcac gcggcgcaag gtgctggaag agcagatcgc
     3841 cttgctgcgc gccgagaaca ctcacatccg cgaggagatc accaacctcg acctgcaggt
     3901 gaaggcgggc gacgccggca tcggctacgc gcgcgagcag ctcgacctca acgagcaact
     3961 gctcagagag aacttcgtcg ccaacgcacg cgtgctcgac ttcaggggcc aactgagcga
     4021 ccggcagcag aagcgcgcgg aagcggccgc gcaggccgcg caggcacgcc agaagatcaa
     4081 gcagaacgaa ctgaagatcg aggggctgcg ccagacctac gtcaaggacg cgaccgacga
     4141 gctgcgtcag gccgagcgcc gcgtcgacga cctgcgcgag cggttgcggc cgacccagga
     4201 cgcgctgtcg cgttcgacga tcgttgcgcc gatcgccggc gtggtggtcg atctgaaggt
     4261 gcacaccgtc ggcggcgtga tcgggccgcg cgaggcgctg atggacatcg tgcccgcgga
     4321 ggcgccgatg atcgtcaagg gcaaggcgcg cgccgaggac atcacgcacc tgcgcgtcgg
     4381 caccgacgtt gccgtgcagt tcaccgccta caagcggcgc accacgccgg tggtcaatgg
     4441 cgcgatcacc tatgtctcgg ccgatgccct ggcggagccg tcgccgctcg ggaccacacc
     4501 gtactacgag gtccatatca gcgtaccgcg cgccgcactg aaggaggcag gcgacctgga
     4561 catcgtgccg ggcatgccgg tggaggcctt cgtgcagacc gaagcgcgaa cggtgctcga
     4621 ctacctgatc cagccgctca cgcagtcgct gcgacgggcc gggcgggaat actgaactga
     4681 accaatgagg cgaggcgcgc tcgagtgctc gcgcgcgtcc gcaggacgac agcaggccca
     4741 cgaaaggaga cacgagccat gagcaccata ttcggaacga ccgccaccga gacgctgaac
     4801 ggcaccgccg gcgacgacac catctacggt ggcccgaccg tcgatcccaa cgcgacgaat
     4861 accggccggg acacgatcaa ctccggcagc ggcaacgata tcgtctacgg ccaggacggc
     4921 gacgattacc tgtacggcca ggacggcggc gacacgctgt acggcggcac cggctacgac
     4981 gcgctgtacg gcggacccgg caatgacgtg ctcgacggcg gcgccgacgg cgactacctg
     5041 accggcggca acacgatgtc cgagagcacc gtcgagatcg gcacgggcga cgaccggctc
     5101 aacggcggcg ccggcaacga cacgctgctg ggtcacgacg gcaacgacgt cctgaacggc
     5161 ggtgacggca acgatcgcct ggtcggccac accggcttca atgtgctcaa cggcgaggcg
     5221 ggcgacgaca cgctgatcag cgcgggcgtc aacgacatgc tcgacggcgg caccttcggc
     5281 gagtcggcca cgctgggcga cctggcgata ctcgatcgca gcaccgcgac cagcgcgctt
     5341 tcactcgccc tcgactcgca ggtgggcaac gcgcagcaga acctcggcga cgggacgctc
     5401 atcaccaaca tcgagcgcct ggacttcacc ggcggtgcgg gcgacgacgt gatcagcggc
     5461 ctgaccgccg gcgactacag cgacgttctg cgcggcggcg cgggggccga cacgctgagc
     5521 gggggcggcg gtatcgacca gctctatggc ggcaccggca acgacatgct cgacggcggc
     5581 gcggggcgcg acaacctgta cggcggcgac acgcagtcgt cgaccgagac caa
//
LOCUS       DBNHBY010000656         2328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1873762, whole genome shotgun sequence.
ACCESSION   DBNHBY010000656 DBNHBY010000000
VERSION     DBNHBY010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1873762"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="AC16HB_15675"
     CDS             complement(<1..1057)
                     /locus_tag="AC16HB_15675"
                     /inference="COORDINATES: protein motif:HMM:NF017727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR5608279.1"
                     /translation="MSAFSAPLWASPFRPFYLLGALYAPLVITGGAGALLGTVHLPTT
                     PQLWHGHEMLFGFALAIVVGTVLTALPSWAGTAEIRGRRLAWLAALWVLGRAAFWASP
                     WLPPWSVALADALLLPVLTAVVAPQLFNLPNRLFLLLLPILVALVAANLAYHASILVG
                     DGAGAERALLAGVYAVIAVFLLKGGVLVPIFTGNALRALGRGDQPPFRVALEAAALLA
                     VVLLGALDVAGASRVAIGTAALACTIVHAVRAARWRGWRVADQPLLASLHLAFAWLIA
                     AFALRAAAELAGIVPAAAWLHAFTVGALGMMMLGLMNRVALRHTGRALDVPLPMRVAY
                     LSMLAAALLRLAATVHGF"
     gene            complement(1062..>2328)
                     /locus_tag="AC16HB_15680"
     CDS             complement(1062..>2328)
                     /locus_tag="AC16HB_15680"
                     /inference="COORDINATES: protein motif:HMM:NF042682.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HIR5608280.1"
                     /translation="LVGMAAHLDGHGVTVLDMTGLAQKGGAVLSHVRVARTQEELATP
                     RIASGAAAALLGCDALVAASADALSKISRGTRAVLNSAAAITGDLVRHPGHALPLDAA
                     IGALRATAGTERVETVDATRLATLLTGQPITVNLFLLGIAWQRGLVPVSRAALARAIE
                     LNGVAVADNALAFEWGRRWAADLSGAERIARDRQQVPDARRLSASLDETIARRRAYLV
                     EYQDERLARRYADLLTRVQAAEQRVAAGSTRLTHAVARAWFKLLACKDEYEVARLYSD
                     PQFAVALARAFEGDWRVRLHLAIPGLARIDPTTGEPRKRAYGPWMLRALRLLARLRRL
                     RGTALDPFRYSAERALDRALVAEYEATIEGILAALRADNLAVAVEIAALPDLIRGYGP
                     VRRRNADAARVRHAELMVQFARAPLAQAA"
BASE COUNT          360 a          830 c          877 g          261 t
ORIGIN      
        1 cgaacccgtg cacggtggcg gccagccgca gcagcgcggc ggcaagcatc gacagatagg
       61 cgacgcgcat cggcagcggc acgtcgagcg cgcggccggt gtgtctcagc gcgacgcgat
      121 tcatcaagcc gagcatcatc atgccgagcg cgccgaccgt gaatgcgtgc agccatgcgg
      181 cggcgggcac gatgccggcc agctcggcgg ccgcccgcag cgcgaacgca gcgatcagcc
      241 atgcgaacgc caggtgcagg gaggcgagca gcggctggtc cgcgacgcgc cagccgcgcc
      301 agcgcgcggc gcgcaccgcg tggacgatcg tgcaggcaag agcggcggtg ccgatcgcaa
      361 cgcgcgaagc gcccgcaacg tcgagcgcgc cgagcagcac gacggcgagc agcgccgcgg
      421 cttcgagcgc gacgcggaac ggcggctggt cgccgcgacc cagcgcccgc agcgcattgc
      481 cggtgaagat cggcaccagc acgccgccct tgagcaggaa cacggcgatc accgcgtaca
      541 cgccggcgag cagcgcgcgc tccgcacccg cgccgtcgcc cacgaggatg gacgcgtgat
      601 acgcgaggtt ggccgcaacg agcgcgacca ggatcggcag cagcagcagg aagagccggt
      661 tgggcaggtt gaagagctgc ggtgcgacca ccgcggtcag cacggggagc agcagcgcgt
      721 cggccagcgc gacgctccac ggcggcagcc acggtgaggc ccagaacgcc gcgcggccga
      781 gcacccacag cgccgcgagc caggccagcc gccggccgcg gatctccgcc gtgccggccc
      841 agctcggcag cgcggtcagc acggtgccga cgacgattgc gagcgcgaag ccgaacagca
      901 tctcgtgccc atgccacaac tgcggcgtgg tcggcaggtg cacggtgccg agcagcgcgc
      961 cggcgccccc ggtgatcacc agcggcgcgt acagcgcgcc gagcaggtag aacggccgga
     1021 acgggctcgc ccacaacggt gcgctgaatg cgctcatcgg gtcaggccgc ctgcgccaac
     1081 ggggcgcgcg cgaactgcac catcagctcc gcgtgccgga cccgcgccgc gtccgcattc
     1141 cggcgccgca ccgggccgta gccgcggatg aggtcgggca gcgcggcgat ttcgaccgcg
     1201 acggcgaggt tgtcggcgcg cagcgcggcg aggatccctt cgatcgtcgc ttcgtactcg
     1261 gcgaccagcg cgcggtcgag cgcgcgctcg gcactgtagc ggaacgggtc gagcgccgtg
     1321 ccgcgcaatc gccgcaggcg cgccagcagc cgcagcgcgc gcagcatcca cggcccgtag
     1381 gcgcgcttgc gcggctcgcc ggtggtcggg tcgatgcgag cgagcccggg gatcgcaagg
     1441 tgcagacgga cgcgccagtc gccttcgaac gcgcgcgcga gcgcgacggc gaactgggga
     1501 tcgctgtaca ggcgcgcaac ctcgtattcg tccttgcacg cgagaagctt gaaccaggcg
     1561 cgcgcgacgg cgtgcgtcag tcgtgtactg ccggcagcaa cgcgctgctc ggccgcctgc
     1621 acgcgcgtga ggaggtcggc gtagcggcgt gcgagccgct cgtcctgata ctcgacgaga
     1681 tacgcgcgac ggcgtgcgat cgtttcgtcg aggctggcgg acagccgacg ggcgtcgggt
     1741 acctgttgcc ggtcgcgcgc aatgcgttcg gcgccgctca gatcggccgc ccagcggcgg
     1801 ccccactcga acgcgagcgc gttgtcggcg accgccacgc cgttcagctc gatcgcgcgc
     1861 gcgagcgcag cgcgcgatac cggtaccagt ccgcgctgcc aggcgatgcc gagcaggaag
     1921 aggttgaccg tgatcggctg gccggtgagc agcgtggcga gccgcgtcgc gtcgacggtt
     1981 tcgacgcgct cggttccggc cgtggcgcgc agcgcaccga tggcggcatc gagcggcagg
     2041 gcatgtccgg gatggcgcac gaggtcaccc gtgatcgccg ctgcgctgtt cagcaccgcg
     2101 cgggtgccgc ggctgatctt cgacagcgca tcggcactcg cggccaccag cgcgtcgcaa
     2161 ccaagcagcg cggcggcggc gccgctggcg atgcgtgggg tggcgagttc ttcctgcgtg
     2221 cgcgcaacgc gcacgtgcga cagcacggcg ccgcctttct gtgccagccc ggtcatgtcg
     2281 agcacggtga cgccgtgccc gtcgaggtgc gcggccatgc cgaccagc
//
LOCUS       DBNHBY010000657         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_791906, whole genome shotgun sequence.
ACCESSION   DBNHBY010000657 DBNHBY010000000
VERSION     DBNHBY010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_791906"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="AC16HB_15685"
     CDS             complement(<1..1099)
                     /locus_tag="AC16HB_15685"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR5608281.1"
                     /translation="MSFELLLRGATIFDGSGAPRRRGNVAVAGGRIAAVGDLRVSAKE
                     TVDLDGLALAPGFIDVHTHDDRLLLADPSMAPKASQGVTTVVVGNCGISLAPLGNCVP
                     VPPLNLVADGGQRFDSFAAYFAALDAQPPAVNCAALVGHTTLRAVTMRDLERPAAGAE
                     IAAMQSHVAEALAAGAIGVSTGTYYAPASAATADEIRRVCEPLRGTNALIASHIRDEG
                     ARVLESMTEAMTIASDLGVRQVLSHHKVIGRANFGRSRETLALLDDVRRTRDVCLDCY
                     PYTASSTVLRADAAQQAQRVLIAWSRQQPRAAGRYLDELAAEQGIAIEALIDALQPAG
                     AIYFSMDEADVERILAYPETMIGSDGLPHDEF"
     gene            1198..>2117
                     /locus_tag="AC16HB_15690"
     CDS             1198..>2117
                     /locus_tag="AC16HB_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608282.1"
                     /translation="MKFQLRTAWRTAAGIALSVGIAAAANVALAQGKVLKFIPEADLR
                     SLDPIWTTAYITRNHGYMVYDVLFATDANFHIQPQMVDKWDASKDGLTYTFTLRDGLK
                     FHYGAPVKSADCIASIERWAKRDVFGQKLAEMTESWTAVNDKTFRLKLKKPFPYVLDA
                     LGKPSSNVPFIMPERVAKTDAFTQITDPTGSGPFKMLKDQWVPGSKVVYVKNTDYVPR
                     KEPPSWGAGGKVVKVDRVEWIYIPDSATAAAALNNGEVDWWQQLPPDLIPVVAKNKDI
                     KVDNTDPLGSIGILRFNHLQPPFNNVKMRQA"
BASE COUNT          373 a          703 c          716 g          325 t
ORIGIN      
        1 gaaactcgtc gtgcggcagg ccgtcggaac cgatcatcgt ctccggatag gcgaggatgc
       61 gctcgacgtc ggcttcgtcc atcgagaaat agatcgcgcc ggccggctgc agggcgtcga
      121 tcagcgcctc gatggcaatg ccctgctccg ctgccagttc atccagatac cgacccgccg
      181 cgcgcggttg ctgccgcgac caggcgatca gaacgcgctg cgcctgttgc gcggcgtcgg
      241 ctctgagcac ggtcgacgac gcggtgtacg gatagcagtc gaggcacacg tcgcgcgtac
      301 ggcgcacgtc gtcgagcagc gccagcgtct cgcgcgagcg gccgaagttg gcgcgcccga
      361 tcaccttgtg gtgcgacagc acctgtcgca cgccgaggtc ggacgcgatc gtcatcgcct
      421 cggtcatcga ttcgagcacg cgcgccccct cgtcgcggat gtgcgaggcg atcagcgcgt
      481 tcgtcccgcg cagcggctcg cacacgcgcc ggatctcgtc cgcggtggcg gcactggccg
      541 gcgcgtagta ggtgccggtc gatacgccga tcgcaccggc ggcaagcgcc tcggcgacgt
      601 gcgactgcat ggcggcgatc tcggcgccgg ccgccggccg ctcgaggtcg cgcatcgtca
      661 ccgcacgcag cgtggtgtgt ccgaccagcg ccgcgcagtt gaccgccggg ggctgcgcgt
      721 cgagcgcagc gaagtaggcg gcaaacgaat cgaaacgctg gccgccatcg gcgacgaggt
      781 tcagcggcgg tacgggtacg cagttaccga ggggcgcgag gctgatgccg cagttgccga
      841 ccacgacggt ggtgacgccc tggctcgcct tcggcgccat cgaggggtcg gcgagcagca
      901 ggcggtcgtc gtgcgtgtgc acgtcgatga agcccggcgc gagtgccagc ccgtcgaggt
      961 cgacggtctc cttcgcacta acccttagat cgccgaccgc cgcgatgcgt ccgcccgcga
     1021 cggccacatt gccgcgccgg cggggcgcgc cgctgccgtc gaagatcgtc gcgccgcgca
     1081 acagcagttc gaagctcatc gtcttttcga aacgacccgg aaacgtgtag tctgtccgcc
     1141 cgctcgcggg ttcgcaaatc gtacgggcgc agcaataacg caatctagga gacagtaatg
     1201 aaattccagc ttcgcaccgc gtggcgaacc gccgccggga tcgcgctttc ggtgggtatc
     1261 gctgcggcgg ccaacgtcgc actggcgcaa ggcaaggtcc tgaagttcat tcccgaggcc
     1321 gacctgcgca gcctcgaccc gatctggacc acggcgtaca tcacgcgcaa ccacggctac
     1381 atggtgtacg acgtgctgtt cgcgacggac gcgaactttc acatccagcc gcagatggtc
     1441 gacaagtggg acgccagcaa ggacggtctc acctacacgt tcacgctgcg cgacggtctg
     1501 aagtttcatt acggcgcgcc ggtgaaatcg gccgactgca tcgcgtcgat cgagcgttgg
     1561 gccaagcgcg acgtgttcgg ccagaaactg gcggagatga ccgagtcgtg gaccgcggtc
     1621 aacgacaaga cgttccggct gaagctgaag aagccgttcc cgtacgtgct cgacgcgctc
     1681 ggcaagccgt cgtcgaacgt gccgttcatc atgccggagc gcgttgccaa gaccgacgcg
     1741 ttcacgcaga tcacggaccc gaccggctcc ggcccgttca agatgctcaa ggaccagtgg
     1801 gtgccgggca gcaaggtcgt gtacgtgaag aacaccgact acgtgccgcg caaggagccg
     1861 ccgtcgtggg gcgccggcgg caaggttgtg aaggtcgatc gcgtcgagtg gatctacatc
     1921 ccggactcgg ccaccgcggc agcggcgctg aacaatggcg aggtggactg gtggcagcaa
     1981 ctgccgccgg acctgatccc ggtcgtcgcg aagaacaagg acatcaaggt cgacaacacc
     2041 gatccgctcg gctcgatcgg catcctgcgc ttcaaccacc tgcagccgcc gttcaacaac
     2101 gtgaagatgc ggcaggc
//
LOCUS       DBNHBY010000658         1906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_521065, whole genome shotgun sequence.
ACCESSION   DBNHBY010000658 DBNHBY010000000
VERSION     DBNHBY010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_521065"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="glcE"
                     /locus_tag="AC16HB_15695"
     CDS             complement(<1..789)
                     /gene="glcE"
                     /locus_tag="AC16HB_15695"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR5608283.1"
                     /translation="MADASLAALQDRVRAAAAAKTPLAIRAGGTKDFYGNAVQGEPLD
                     PRAHAGIVAHEPTELVITARCGTRLAELEVALAAAGQMLAFEPPQFGAPATLGGCVAA
                     GLSGPRRASAGAVRDFVLGVKLLDGRGDVLRFGGTVMKNVAGYDVSRLLAGSLGTLGV
                     ILEVSLKVLPQPPAETTLRFELDEASALRRANEWAGQPLPISATAWCGGTLTVRLSGA
                     RAAVEAARAKLGGERVDDMQAASFWRGLREHTDPYFVGNAPLWRL"
     gene            complement(789..>1906)
                     /locus_tag="AC16HB_15700"
     CDS             complement(789..>1906)
                     /locus_tag="AC16HB_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5608284.1"
                     /translation="RNLAITEAAAPHGLYYAPDPSSQIACTIAGNVAENSGGVHCLKY
                     GLTFHNVLRVRGVTSDGEILEVGSDALDAPGFDLLALVIGSEGMLAVVTEVTVKLVPK
                     PQLARCIMASFADVPTAGNAVAAIIAAGLIPAGLEMMDQPATRMVEPFVKAGYDLDAA
                     SILLVESDGAPEEVEEEIARMKDVLTANGATKIAVSNSEAERLRFWAGRKNAFPAAGR
                     VSPAYYCMDGTIPRKRLGEMLAAIQQMEGKYGLRCPNVFHAGDGNLHPLILFNDNDPD
                     EVQRAEDFGVEILELSVALGGTITGEHGVGVEKIAQMCVQFSEAERALFHAVKRAFDP
                     AGLLNPDKAIPTLHRCAEYGRMKVSGGKLKFPELERF"
BASE COUNT          289 a          687 c          635 g          295 t
ORIGIN      
        1 gagccgccac agcggcgcgt tgccgacgaa gtacggatcg gtgtgctcgc gcaggccgcg
       61 ccagaacgac gccgcctgca tatcgtcgac gcgttcgccg ccgagcttcg cgcgcgccgc
      121 ctcgaccgcc gcgcgcgcgc cggaaagccg cacggtgagc gtcccaccgc accacgcggt
      181 ggccgagatc ggcagcggct ggccggccca ctcgttggcg cggcgcagcg cgctcgcctc
      241 gtccagttcg aagcgcagcg tcgtttcggc gggcggctgc ggcagcacct tcagcgacac
      301 ttcgaggatc acgccgagcg tgccgaggct gcccgcgagc aggcgagata catcgtagcc
      361 ggcgacgttc ttcatcaccg tgccgccgaa gcgcagtacg tcgccgcggc cgtcgagcag
      421 cttcacgcca agcacgaaat cgcgcacggc accggcgctc gcacggcgcg gccccgacag
      481 ccccgcggcc acgcagccgc cgagggtggc cggggcgcca aactgcggcg gttcgaacgc
      541 gagcatctgt cccgccgccg cgagcgcgac ctcgagctcg gcaagccgcg taccgcagcg
      601 cgcggtgatc acgagttcgg tcggctcgtg ggcgacgatg ccggcatgcg cgcgcggatc
      661 gagcggctcg ccctggaccg cgttgccgta gaagtccttg gtgccgccgg cgcggatcgc
      721 aagtggcgtc ttcgccgcag cggcggcgcg cacgcgatcc tgcagcgcgg ccagcgacgc
      781 atcggccact agaaccgctc cagctccgga aacttcagct tgccgccact gaccttcatg
      841 cgaccgtact cggcgcagcg atgcagcgtc ggaatcgcct tgtccggatt cagcagcccg
      901 gccggatcga acgcgcgctt gactgcgtgg aacagcgcgc gctcggcttc gctgaactgc
      961 acgcacatct gcgcgatctt ctccacgccg acaccatgct cgccggtgat cgtgccgccg
     1021 agcgccacgc tcagttccag gatctcgacg ccgaagtctt cggcgcgctg cacttcgtcc
     1081 ggatcgttgt cgttgaacag gatcagcgga tgcaggttgc cgtcgccggc atgaaagacg
     1141 ttcgggcagc gcagtccgta cttgccttcc atctgctgga tcgcggcaag catttcgccc
     1201 agccgcttgc gcgggatcgt gccgtccatg cagtagtacg ccggcgacac gcggccggcg
     1261 gcgggaaacg cgttcttgcg cccggcccag aacctcagcc gctcggcctc cgagttcgac
     1321 accgcgattt tcgtcgcgcc gttcgcggtc agcacgtcct tcatgcgcgc gatctcttcc
     1381 tcgacttcct ccggcgcgcc gtcggactcg accagcagga tcgacgccgc gtcgaggtcg
     1441 tagccagcct tcacgaacgg ctcgaccatc cgtgtcgcgg gctggtccat catctccagt
     1501 cccgccggga tcagtccggc cgcgatgatc gccgcgacgg cgttgccggc ggtgggcacg
     1561 tcggcgaagc tcgccatgat gcagcgggcg agttgcggct ttggcaccag cttgaccgtc
     1621 acctcggtaa ctaccgcgag catcccttcg gagccgatca ccagcgcgag caggtcgaac
     1681 cccggagcat cgagcgcgtc ggagccgact tccaggatct cgccgtcgct ggtcacgccg
     1741 cgtacgcgca agacgttgtg gaacgtcaga ccgtacttga ggcagtgcac gccgccggaa
     1801 ttctcggcga cgttgccggc gatggtgcag gcgatctgac tcgaaggatc gggcgcgtag
     1861 tacaacccgt gcggcgcggc cgcttcggtg atcgccagat tgcgcg
//
LOCUS       DBNHBY010000659         2412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_385831, whole genome shotgun sequence.
ACCESSION   DBNHBY010000659 DBNHBY010000000
VERSION     DBNHBY010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_385831"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="AC16HB_15705"
     CDS             complement(<1..956)
                     /locus_tag="AC16HB_15705"
                     /inference="COORDINATES: protein motif:HMM:NF005043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR5608285.1"
                     /translation="MNDPGTLVVRAILLIPAACALLLALLPDDRTTARLNVLGTLATL
                     LCALALFVVRPLPGPFLIVDDVNVVFIVLNTFVSFTTSVFSASYIAHELEIGRLTHAY
                     LRFYHAMYQTLMFAMNLALLANNVGLMWVAIELATLTTVLMVGIYRTHEALEAAWKYF
                     ILGSVGIALALFGTILVYLAASSVLGEGLDAMAWSVLVQRAPDFDPALLNLAFVFLLL
                     GYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALFALLRFKMVMSANEHALAP
                     GPLMVSLGLASVVFAAFMLYRRDDIKRMFAYSSIEHMGIISFA"
     gene            complement(1114..1776)
                     /locus_tag="AC16HB_15710"
     CDS             complement(1114..1776)
                     /locus_tag="AC16HB_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR5608286.1"
                     /translation="MDRLAFDIAHLFAGSLVLVSFMLLYQDRMFSLLNVFALHALVVS
                     VSVAWQAHIQSASHLYITAAIALVVKAIVIPVALHRIIVRLGIHRDIETVVGIGQIML
                     GGIALVGLSILVMLPITTHAGTLVREDLAFALAVILLGLLMMVSRRNAVGQVIGFMSI
                     ENGLILAATGARGMPLVVEISVAFSILIALIVIGIFLFRIRERFDSVDVHALDRVRVE
                     RQ"
     gene            complement(1776..>2412)
                     /locus_tag="AC16HB_15715"
     CDS             complement(1776..>2412)
                     /locus_tag="AC16HB_15715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR5608287.1"
                     /translation="LGSARFMLALAGLDVGTSFGGIGSSREMMIASLAEPALLMIAFT
                     VSLLAGSTQLSSVATFMQGPDVGLRVSLAMALVALVMVAIAENARIPIDNPATHLELT
                     MVHEAMILEYSGRHLAVIEAAASLKLLLYLSVIGCVFVPWGVAVAGEGPATYAVALVV
                     YTAKLAVAGLLLAVFETVIAKMRVFRVSDFLGAALMLGLLATLLLFVSRSG"
BASE COUNT          496 a          790 c          806 g          320 t
ORIGIN      
        1 gcgaagctga tgatgcccat gtgctcgatc gaggaatagg cgaacatgcg cttgatgtcg
       61 tcgcggcggt agagcatgaa ggccgcgaac acgaccgaag ccaggcccag gctcaccatc
      121 agcggccccg gcgccagcgc atgctcgttg gccgacatca ccatcttgaa gcgcagcagg
      181 gcaaacagcg cgacattgag cagcagcccc gacagcaccg ccgaaatcgg tgtcggacct
      241 tccgcatgcg catcgggaag ccatgcgtgc agcggtgcca ggccgacctt ggtgccgtag
      301 cccagcagca gaaagacgaa cgccaggttg agcagcgccg gatcgaagtc cggggcgcgc
      361 tgcacgagga cgctccacgc cattgcatcc agaccttcgc ccagcaccga actcgccgcg
      421 agatacacga ggatggttcc gaacagcgcc agcgcgatgc cgacgctgcc caggataaag
      481 tacttccatg cggcttcgag cgcttcgtgg gtgcgataga tgccgaccat cagcaccgtg
      541 gtgagggtcg ccagttcgat ggcgacccac atcagcccga cgttgttcgc cagcagcgcc
      601 agattcattg cgaacatcag cgtctggtac atcgcgtgat agaagcgcag gtacgcatgc
      661 gtcagccggc cgatctcgag ttcgtgggcg atgtagctgg cgctgaagac gctggtggtg
      721 aagctgacga acgtgttgag cacgatgaac acgacgttca cgtcatcgac gatcaggaac
      781 gggccgggca gcgggcgcac gacgaacagg gcgagcgcgc acagcagcgt cgcgagcgtg
      841 ccgaggacgt tgagccgcgc ggtcgtgcga tcgtccggca gcagcgcgag cagcagcgcg
      901 cacgcggccg ggatcagcag gatcgcgcgg acgaccaggg tgcccggatc gttcactgtt
      961 gccccctacg gcgcttcgcg ccggtccccc caagggggcc gaggtccgcg gctccatgcc
     1021 cgcctgcgcg ggcatggacg gaccgaggag acgtcgcgtc tcgcgacgcc gcggcctgca
     1081 aggccgagcg ccttcggagc ggccgtgcgg cgctcattgc cgttccaccc ggacgcggtc
     1141 gagcgcatgc acgtcgaccg aatcgaagcg ctcgcgaatg cggaaaagga agatgccgat
     1201 cacgatcagc gcgatcagga tcgaaaaggc cacgctgatc tcgaccacga gcggcatgcc
     1261 gcgcgcgccg gtggcggcca ggatcagccc gttctcgatc gacatgaacc cgatgacctg
     1321 tcccacggcg ttgcggcgcg acaccatcat cagcagtccg agcaggatca ccgccagggc
     1381 gaaggcgagg tcctcccgca ccagcgtgcc cgcgtgggtc gtgatgggca gcatcacgag
     1441 gatcgagagc ccgaccagcg cgatgccgcc cagcatgatc tggccgatgc cgaccacggt
     1501 ttcgatgtcg cgatggatgc cgagccgcac gatgatgcgg tgaagcgcca ccgggatgac
     1561 gatggccttg acgacgaggg cgatcgcggc cgtgatgtac aggtgcgacg cgctctggat
     1621 atgcgcctgc cacgccaccg agaccgagac cacgagcgcg tgcagtgcaa acacgttcag
     1681 caggctgaac atgcggtcct ggtacagaag catgaagctg acgaggacga gactgccggc
     1741 gaacagatgg gcgatgtcga acgcgaggcg atccatcatc cgctccgcga gacgaacagc
     1801 agcagcgtgg cgagcagacc gagcatcagt gccgcgccca ggaagtccga cacgcggaac
     1861 acccgcatct tggcgatcac ggtctcgaac accgccagca ggaggccggc caccgcgagc
     1921 ttcgcggtgt agacgacaag agcgaccgcg taggtcgcgg ggccttcgcc ggccacggcc
     1981 acgccccacg gcacgaacac gcagccgatc acggacagat acagcaggag cttcagcgac
     2041 gcggcggcct cgatcaccgc cagatggcgc ccggagtatt cgaggatcat cgcctcgtgc
     2101 accatcgtga gctcgaggtg ggtggcggga ttgtcgatcg ggatgcgcgc gttttcggcg
     2161 atcgccacca tcacgagcgc gaccagggcc atcgccagcg agacccgcag cccgacatcg
     2221 gggccctgca tgaaagtggc gaccgaggaa agctgggtcg aacccgccag cagcgaaacc
     2281 gtgaaagcaa tcatcagcag cgccggctcg gcgagcgacg cgatcatcat ttcgcggctc
     2341 gacccgatgc cgccgaaact ggttccgaca tccagccccg ccagcgcgag catgaaacgc
     2401 gcgcttccca gc
//
LOCUS       DBNHBY010000660         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1468074, whole genome shotgun sequence.
ACCESSION   DBNHBY010000660 DBNHBY010000000
VERSION     DBNHBY010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1468074"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16HB_15720"
     CDS             <1..207
                     /locus_tag="AC16HB_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608288.1"
                     /translation="PAQVPARVRVPAWARARARVPARVLVLVLVRVLVLVLVVLLAQL
                     DRRPLVPLLNRRRKLQPRWCRWML"
     gene            complement(869..1414)
                     /locus_tag="AC16HB_15725"
     CDS             complement(869..1414)
                     /locus_tag="AC16HB_15725"
                     /inference="COORDINATES: protein motif:HMM:NF040152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HIR5608289.1"
                     /translation="MSTDSSTPSPAHSATLSDDRVISSLELNLTRLVSTGAIADARGG
                     FIMSINIAMLGALAASVAGSTMLSKGIGAWLLFLAAGGFSLASMGAVVLGAFPRVHAS
                     GPPSLAFFQGISSFTSDAYYERLRSISRAEYARDLSDNCHRVAKIIAIKFRYARIGIV
                     MLILAAGPWVGCLFLAGRLPI"
     gene            1578..>2224
                     /gene="pepN"
                     /locus_tag="AC16HB_15730"
     CDS             1578..>2224
                     /gene="pepN"
                     /locus_tag="AC16HB_15730"
                     /inference="COORDINATES: protein motif:HMM:NF037667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR5608290.1"
                     /translation="MPTTSMRNDQAVTIHRADYRPPHFLVESIALEFDLDPQLTRVTA
                     TLQVRRSEPGSTAPLVLDGEDLELESIELDGRRLESGFRVQDGTLTIDAVGDRARLRT
                     VTRIRPQDNTELMGLFVSHGNFFTQCEAEGFRRITYFPDRPDVMTVYTVTLRADGERY
                     PVLLANGNLVAEGRLADGRRYARWHDPFPTPSYLFALVAGHFECNEARLRTRSGR"
BASE COUNT          388 a          742 c          721 g          373 t
ORIGIN      
        1 ccggcgcagg tgccggcgcg ggtgcgggtg ccggcgtggg cgcgggcgcg ggcgcgggtg
       61 ccggcgcggg tgctggtgct ggtgctggtg cgggtgctgg tgctggtgct ggtggtgttg
      121 ctggcgcaac tggaccggcg accgcttgtt cccctgctaa accgccgccg caagctgcaa
      181 ccgcgatggt gccgatggat gctgtagcgg cagtcaagaa tgtgcgccgc gtgatcggtg
      241 tccgtcggac tggaattgct cgatttactt tcatgtctcc tctgcgaacc gatccctcgc
      301 gccacgacct tcagtcaggt cgagattgtc gagcagagcc aacgcatgct tgtcacctcg
      361 acaagctaat cctcatgtcg tgtagcaacg ttgtcgcgtt gtgaatcaga ccgaggcgtc
      421 actgccgcgc gccctgagaa tcattcagac gatcggacga cgcgcccgca tggatccggc
      481 tcttgttcgt tcagccgcga gcgtccacga cttgagcaat cccgtcattc cgctctggcg
      541 acaatttctg gagctgcagc ctcaccgaac cgtaaccggc tcgtcggcca tttcgcgcga
      601 cccgcgccac ctgcgcatcg gtcacgaggg ccacgactgg acatcaacgt tcggcacgcg
      661 agacatgaca tcgcgatctg ccggggcccg atgacggcga cctatgccta tggagggtgg
      721 cgcggtttat ctggactgca cccctgtgga gtcgcacatg gacactggcg gtttgagaga
      781 cgtcacctcc aacgaacaag aacgccacct tgcggtggcg ttcttctttg ccgcaaggcg
      841 cggctcgaat cccgcgcttc tgcttccctc agatcggcag ccgccctgcc aggaacaggc
      901 agccgaccca cggcccggcg gcgaggatca gcatcacgat gccgatgcgc gcatagcgaa
      961 acttgatcgc gatgatcttc gcgacccggt ggcagttgtc ggacaggtcg cgcgcgtact
     1021 cagcgcggga aatgctccgc agccgctcgt agtaggcgtc gctggtgaaa ctgctgatgc
     1081 cctggaagaa ggccagggat ggtgggccgc tcgcatgcac gcgcggaaag gcgccgagca
     1141 cgaccgcccc catgctggca agactgaagc cgccggcggc caggaacagc agccaggccc
     1201 caataccctt cgacagcatc gtgctgcccg ccacggacgc ggcgagcgcg ccgagcatcg
     1261 caatgttgat cgacatgatg aatccgccgc gcgcatcggc gatcgcgccg gtcgacacga
     1321 gccgggtgag attcagctcc aggctcgaaa tcacgcggtc gtccgacagc gtcgccgaat
     1381 gagccggcga gggcgtggat gagtccgttg acatggcgaa tcgcgtcggt tcccgcgaac
     1441 gcctcggtcg tgcggatcgc ggagcgaggc gaagcctagg aggacttcgc ggccgaagca
     1501 aggtcatacg cgaagagcag cggccgcggc caacggcacc ccgaagatag aatcgcctgc
     1561 atcggtcccg cgcccgcgtg ccgaccacat cgatgcgcaa cgaccaggcc gtcacgatcc
     1621 accgcgccga ttaccggccg cctcatttcc tcgtcgagtc gatcgcgctc gagttcgatc
     1681 tcgatccgca actgacgcgc gtcaccgcca cgctgcaggt ccggcgcagc gagccgggtt
     1741 cgaccgcgcc gctcgtgctc gacggtgagg acctcgaact cgaatcgatc gaactcgacg
     1801 gccgccgcct cgagtccggc tttcgcgtgc aggacggcac gctgacgatc gacgcggtcg
     1861 gcgaccgcgc acggctccgc accgtcacgc gaatccggcc gcaggacaac accgaactga
     1921 tgggcctgtt cgtctcgcac ggcaatttct ttacccaatg cgaggccgaa ggcttccgcc
     1981 gcatcacgta ctttcccgac cgaccggacg tgatgacggt ttacaccgtg acgctgcgcg
     2041 ccgacggcga gcgctatccg gtactgctcg ccaacggcaa tctcgtcgcg gaagggcgac
     2101 ttgccgacgg ccgccgctat gcgcgctggc acgatccgtt tccaacgccc agctaccttt
     2161 tcgcgctagt ggcgggccac ttcgagtgca acgaggcaag gttgcgcacg cgctcggggc
     2221 gcga
//
LOCUS       DBNHBY010000661         3330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1806656, whole genome shotgun sequence.
ACCESSION   DBNHBY010000661 DBNHBY010000000
VERSION     DBNHBY010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1806656"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3293)
                     /locus_tag="AC16HB_15735"
     CDS             complement(<1..3293)
                     /locus_tag="AC16HB_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5608291.1"
                     /translation="MIAQRELLGLLYADIGGLYGRFHDGDTQLISMLAAQAALTLANV
                     RAAEGLERKVASRTAELNHRVRELEVISTIQRGVAGQLDFQTIVDLVGDKLREVMRSQ
                     DIGISWYDPRTNLAHPLYVYEHGKRLHEQPHPLRPGGPGERMLATREPVVINSQAELV
                     AIVGGPMPGTDLARCAVWVPIISSDRVLGSVRLENHDREFAYGESEVRLLRTVAASMG
                     VALENARLFDETQRLLKETEQRNAELAVINSIQQGIAGSLEFQVIVDLVGDKLREMLK
                     TNDIGIWWLDRQTKLGLGLYFYEHGRRLSVAPQPVPAPIARMMATRQPLIYRSTSEML
                     ADGLTAVPGTDQSKSAISVPILGADGMVGCVNLEDYERENAFGEAELRLLQTVAASMG
                     VALENARLLEETQRRARESSALSEVGRDLSSTLDLARVMDRIARHAKDLLAASNSAIF
                     LPDANGETYRAIVALGEVAEQLKATTVEAGRGIIGSLLQSGQAEFINDTHADPRGVQI
                     AGTQQRRDERLMVVPLLADEAVQGAMAVWRSGGQPFDQRDLEFLVGLSRQAAVALRNA
                     QLFDETRRSLDQQTATAEVLEVISQSVADAAPVFEKIVECCERLFSARAFAIGIVDDR
                     GQVALPVYRLTAQGRTLVGNEKADAIEARTRASFPRPLAGTLTEMVIRAGRLLEIRDV
                     LDDPNASQPAVRAAAEMGLGTSVVVAPLLWEGRGIGSLTMMRDARDGLHEREKALLKT
                     FSEQAVIAIQNARMFTETREALEHQTATAEVLQVISGSMADATPVFEKILDSCARLFG
                     ATDLGVFLVDAQERLNVGAYRGSFADWVPSTYPRPLAGTMSEMVIRNGSLMHWADVEH
                     AQDIPGYIRAVVRDSGNFSVAVAPLLWEGRGIGTIDVMRKPPRAYTEKELALLATFAD
                     QAVIAIQNARLFNETREALERQTATAEVLQVISESPTDVQPVFDVIAERSARLTGADY
                     GVVLRFDGEWLDVVSLFGLKADGVAAVRSAFPMRAEGGSISARAVNTRDVVNVPDMLE
                     DPLLSAQVREIVARAGYRSGLSVPMLRGEQIVGAITVNRTLPGAFAAKEVELLRTFAS
                     QAVIAIENVR"
BASE COUNT          523 a         1157 c         1059 g          591 t
ORIGIN      
        1 cgcacgttct cgattgcgat cacggcctgg ctggcgaagg tccgcagcag ttccacctcc
       61 ttggcggcaa aggcaccagg cagtgtccgg ttgacggtga ttgcacccac gatctgctcg
      121 cctcgcaaca tcggcaccga cagcccgctg cggtaacccg cgcgcgcgac gatctccctg
      181 acctgggccg acagcagggg gtcctcgagc atgtcgggca cgttgaccac gtcgcgcgtg
      241 ttcactgccc gcgccgagat cgagccgccc tcggcacgca tcggaaaggc gctgcggact
      301 gcggccacgc cgtccgcctt caggccgaac agactgacca cgtcgagcca ttcgccgtcg
      361 aagcgcagca cgacgccgta gtccgcgccg gtcagccgag ccgagcgctc ggcgatcacg
      421 tcgaacaccg gctgcacgtc ggtcggcgat tcgctgatca cctgcaacac ctcggccgtg
      481 gccgtctgcc gctccagcgc ctcgcgcgtt tcgttgaaca gccgcgcgtt ctggatcgcg
      541 atcaccgcct ggtcggcgaa agtagcgagc agcgccagtt ccttctccgt gtacgcacgc
      601 ggcggcttgc gcatcacatc gatcgtgccg atgccgcgtc cctcccacag cagcggtgcg
      661 acggcaaccg agaaattgcc gctgtcccgc accactgcgc ggatgtagcc gggaatgtcc
      721 tgcgcgtgtt ccacgtcggc ccagtgcatc aacgatccgt tccggatcac catctcgctc
      781 atcgttccgg ccagcggacg cggatacgtg ctcggcaccc agtcggcaaa gctgccgcgg
      841 taggcgccca cgttcagacg ctcctgcgcg tcgacgagga agacaccgag atccgtggcg
      901 ccgaacagcc gtgcgcagct atcgaggatt ttctcgaaca ccggcgtcgc gtcggccatc
      961 gagccgctga tgacctgcag cacctcggcc gtcgcggtct ggtgttcgag cgcctcgcgg
     1021 gtctcggtga acatgcgcgc gttctggatc gcgatcacgg cctgctcgga aaatgtcttg
     1081 agcagcgcct tttcacgctc gtgcaggcca tcgcgcgcat cgcgcatcat cgtcagtgaa
     1141 ccgatgccgc ggccttccca cagcagcgga gcgacaacca ccgaggtgcc caatcccatt
     1201 tctgcagccg cgcggacggc gggttgcgat gcgttcggat cgtcgagcac atcgcggatc
     1261 tcgagcagtc ggcccgcacg gatgaccatt tcggtcaggg tgccggcgag aggtcgggga
     1321 aaggatgccc gcgtccgggc ttcgatggcg tcggccttct cgttgccgac cagcgtcctg
     1381 ccttgcgccg tcaggcgata caccggcaag gcaacctgac cgcggtcatc gacgatgccg
     1441 atcgcgaacg cacgcgccga gaacaggcgt tcgcaacact cgacgatctt ctcgaacacg
     1501 ggtgccgcat cggccaccga ctggctgatc acctccagca cttcagcggt cgcagtctgc
     1561 tggtcgagcg acctgcgagt ctcgtcgaac agttgcgcgt tccgcagcgc gaccgcagcc
     1621 tggcgtgaca ggccgacgag gaactccagg tcgcgttgat cgaagggctg gccgccgctg
     1681 cgccagaccg ccatcgcacc ctgcaccgct tcgtcggcga gaagcggcac caccatcagc
     1741 cgctcgtccc tgcgctgttg tgtgcccgcg atctgcaccc cacgcggatc cgcgtgggtg
     1801 tcgttgatga attcggcctg gccgctctgc agcaggctgc cgatgatgcc gcgccccgcc
     1861 tcgacggtcg tggccttgag ttgctcggcc acttcgccga gcgccacgat ggcgcgatag
     1921 gtctcgccat tcgcgtccgg caggaagatc gcgctgttgc tggcagcgag caggtccttg
     1981 gcgtgacgcg cgatgcggtc catcacccgg gcgagatcca gtgtggacga caagtcgcgg
     2041 cccacctccg acagtgccga cgattcgcgc gcgcggcgct gtgtctcttc tagcagtcgc
     2101 gcgttttcga gcgccacgcc catcgatgcg gcaacggttt gcagcagtcg tagctccgcc
     2161 tcgccaaatg cgttctcgcg ttcgtagtct tccagattca cgcagccgac catgccgtcg
     2221 gcgccgagga tcggaacgct gatggccgac ttgctctggt ccgtaccggg gacggccgtc
     2281 aggccatcgg ccagcatctc cgacgtgctg cggtatatca gcggctgtcg tgtcgccatc
     2341 attcgcgcga tcggcgccgg caccggctgc ggcgcgacac tgagccgtcg tccgtgctcg
     2401 tagaagtaca gacccagccc cagcttcgtt tgccgatcga gccaccagat gccgatatcg
     2461 ttcgtcttca gcatctcgcg cagcttgtcg ccgacgagat cgacgatgac ctgaaattcg
     2521 agcgagccgg cgattccctg ctggatgctg ttgatcaccg ccaactcggc gttgcgctgc
     2581 tcggtctcct tgagcagccg ttgcgtctcg tcgaacaggc gcgcgttctc gagcgccacg
     2641 cccatgctcg cggcgaccgt tcgcagcagg cgcacctcgg attcgccgta ggcaaattcg
     2701 cgatcgtggt tctcgagtcg cacgctgccg agcacgcggt cgctgctgat gatgggcacc
     2761 cacaccgcgc acctggccag gtcggttccg ggcatgggcc cgccgacgat ggcgacgagt
     2821 tccgcctggc tgttgataac gacaggctcg cgcgtggcca gcatgcgctc gcccgggcca
     2881 ccgggccgca acgggtgcgg ttgctcgtgc aggcgtttgc cgtgctcgta aacgtagagc
     2941 ggatgcgcca gattcgtccg cgggtcgtac cagctgatac cgatgtcctg gcttcgcatc
     3001 acttcgcgca gcttgtcgcc gacaagatcg acgatggtct ggaaatcgag ctgccccgcc
     3061 acgccgcgct ggatcgtgct gatgacttcc agctcgcgca cgcggtggtt cagctccgca
     3121 gtgcggctcg caaccttgcg ttccagtcct tcggccgccc gcacgttggc gagcgtcagc
     3181 gcggcctgcg cggcgagcat gctgatcagc tgcgtgtcgc cgtcgtggaa gcggccgtag
     3241 aggcccccga tgtctgcgta cagaaggccc agcagttcgc gctgcgcgat caacgggacg
     3301 atgaggcaac tgcgctgatc gatcggctcg
//
LOCUS       DBNHBY010000662         3152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1536848, whole genome shotgun sequence.
ACCESSION   DBNHBY010000662 DBNHBY010000000
VERSION     DBNHBY010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1536848"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16HB_15740"
     CDS             complement(<1..301)
                     /locus_tag="AC16HB_15740"
                     /inference="COORDINATES: protein motif:HMM:NF037184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608292.1"
                     /translation="MPSADTYLALEGPGALSAFRAARLLARIRAIEPDAAAIRARHMH
                     FVHCTGALRRDDRGRLEGLLTYGETPDADPRGGAQWLVVPRIGTISPWASKATDIA"
     gene            complement(402..3152)
                     /locus_tag="AC16HB_15745"
                     /pseudo
     CDS             complement(402..3152)
                     /locus_tag="AC16HB_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665533.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
BASE COUNT          494 a         1090 c         1055 g          513 t
ORIGIN      
        1 gcgcgatgtc ggtcgccttg ctggcccacg gcgagatcgt gccgatgcgc ggcacgacca
       61 gccattgcgc tccgccccgg ggatcggcat ccggcgtttc gccgtaggtc agcagccctt
      121 cgagccggcc gcgatcgtcc cgtcgcagcg cgcccgtgca gtgcacgaag tgcatgtgac
      181 gcgctcggat cgcggcggca tcgggttcga tggcgcgaat ccgcgccagc agccgcgcgg
      241 cacgaaatgc ggacagcgcg cccgggcctt cgagcgcgag atacgtatcg gcggatggca
      301 tgcggaggag gcgctccgcg aggaagagaa cgagacggcg gtagcgcggc gcgaagtata
      361 gccgcgcagt ccgatgcgga tcactgccta tcctgccgcg atcaggccgc cttgccgaaa
      421 ccgccgccgc ccggcgtctc gatcacgaac acgtcgcccg gattcatctc gcggctgccg
      481 atgtgcggca gttcctcgat cgagccgtcc gtgcgctcga cccagttgcg gccggccgcg
      541 cccggatcgc cgcccgccag gccgaacggc ggcatggcgc ggctgttgga caggatcgaa
      601 gcggtcatcg gctcgaggaa cttgacgcgt cgacggccac cgtcgccgcc gcgccatttg
      661 ccgcggccgc cggaatcgcg ccgtatctcg tagcgctcca gacgcacggg gaagcggaac
      721 tccagcacct ccgggtcggt caggcgtgag ttggtcatgt gtgtctgcac gacgtcggtg
      781 ccgtcgaagc cttccttcga cgccgcgccg gcggccagat cggtgatgcc ggcgccggaa
      841 ccccccgaga tcgtctcgta gtactggtag cgcgcgttgc cgaacgtgaa attgttcatc
      901 gtgccctgcg acgacgcaag cacaccgagc gcaccgaaca gcgtgttggt gatgcacgtc
      961 gacgtttcga cgttgcccgc gacgactgcg gaggggtagc gcggactgag catcgaaccc
     1021 tccggaatga cgatcttcaa cggtttcagg caacccgcgt tcagcggaat ctcgtcatcg
     1081 accagtgtgc ggaacaccta cagcacggcg gcgacacaga tgctcgacgg cgcattgaag
     1141 ttgttgggca actgcggcga ggtaccgctg aagtcgatca ccgcagagcg cgatccgcgg
     1201 tcgacgcgga tcgccacccg gatgcacgcg tcgttgtcga gcctgtagtc gaacgcaccg
     1261 tcgcgcagca cggtgatcac gcgccgcacc gactcctcgg cgttgtcctg cacgtgccgc
     1321 atgtacgcct gcacgacgcc gaggccgaac tggtcgacca tccgcagcag ttcctcgcgt
     1381 cccttctcat tggccgcgat ctgcgccttc agatcaccga tgttctgttc gggattgcgc
     1441 gcggggtact tgccgctcgc cagcacggcg cgacattcgg cctcgcgcag ccggccgggt
     1501 cgctccgcgc cttgcccctc gacgagatgg aaattcgtga acagcacacc ctcctcgtcc
     1561 agatgcgagg agaacggcgg catcgaaccg ggcgtgatgc cgccgatgtc ggcgtggtgc
     1621 ccgcgcgaac cgacgtaaaa caggatctcg cgacccgcgc ggtcgaacac gggcgtgatc
     1681 acggtgacat cgggcaggtg cgtgccaccg tggtacggat cgttgacgac gaacgcgtcg
     1741 cctggcgtca tcttcccggc gttctctcgc atcaccgtct tgatcgactc gctcatcgag
     1801 cccaggtgca ccggcatgtg cggcgcgttg gcgatcaggt tgccttcgcc gtcgaacagt
     1861 gcgcaggaga agtcgagccg ctccttgatg ttgaccgagt aggcggtgtt ctgcagtcgc
     1921 agccccatct gctcggcgat cgacatgaac aggttgttga agatctccag cagcaccggg
     1981 tcgacctgcg tgccgatcgc tgtgcgtttc gcgcgcggct cgacgcgctt catcaccacg
     2041 tggtcgagcg gcgtgatctc ggcgcgccag ccgggctcga cgaccgtcgt cgcgttggcc
     2101 tcggcgatga tggccggtcc gtcgatcgcg tcgccgggcc gcgtgtcggc acgcacgaag
     2161 agcggcgtct cgtgccaccc gccgtcgaaa taggcgcgca ccgaggtcgc cgcgcgcagc
     2221 cgaccactgc gcggtgcagt ctcgaccggc cgctcggcac caggttcgcc ggcgccgatg
     2281 gcctcgaccg acaccgcctc gacgatcagc gcgcggtccg gcatcaggaa cgaatagcgc
     2341 tgtgcgtacg cgtcgtcgta ctgcacgcgc atcgcttcca gcgtcccaaa accgacgatc
     2401 aaggcagagt cggtgccctc gtagcgcagg tgcgcgcgtt tcacgcaaga gatccgctcg
     2461 cgcgccacgc cctggttcac gagttcctcg gtggccgacg cttccagctt ggccagcgtg
     2521 gcgtcgagcg ccgccatcgc ctcgggcgcg agcttcgact cgaccgcggt ctcgcgcatc
     2581 gcggtctggt ccgcgagccc catgccgtag gcggacagga cgccggccag tgggtgggcg
     2641 aagatcgacg tcatcgcgag ctggtccgcg accgcgcagg cgtgctggcc gccggcaccg
     2701 ccgaacaccg tgagcgtgta tttggtcacg tcgtgaccgc gctgcaccga gatgcgcttg
     2761 atcgcttcgg ccatgttgcc gaccgcgatg tcgatgaagc cggcggccac ctgctccggc
     2821 gtcttcttct gcccggtcgc gcgctcgatt tcggccgcga gctcggtgaa cttgcgcacg
     2881 acaacctcgc gatcgagcgg ctggtcggcg cgcgggccga atacatgtgg aaaatggtcg
     2941 ggctggatgc ggccgagcat tacgttggcg tcggtcaccg cgagcggccc gccgcgccga
     3001 tagcaggccg gccccggatt ggcgccggcc gaatccggcc ccacgcgcag ccggctgcca
     3061 tcgaagtgca agatcgagcc gccgcccgcc gcaaccgtgt ggatgctcat catcggcgca
     3121 cgcatccgca cgcccgccac caacgtttcg aa
//
LOCUS       DBNHBY010000663         2207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1604416, whole genome shotgun sequence.
ACCESSION   DBNHBY010000663 DBNHBY010000000
VERSION     DBNHBY010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1604416"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16HB_15750"
     CDS             <1..315
                     /locus_tag="AC16HB_15750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5608293.1"
                     /translation="HRGDISQVYVDPARRGLAVGSKLMRRTLALAFELPGLEQVELTV
                     VSTNTAAVRLYQRLGFVQFGLQENYFKVGELRWNMCLMQLSRASYLAGNRPASTARSQ
                     HA"
     gene            308..616
                     /locus_tag="AC16HB_15755"
     CDS             308..616
                     /locus_tag="AC16HB_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HIR5608294.1"
                     /translation="MLEMRPTCENCNRALPPDSLEARICSFECTFCATCATGVLRNVC
                     PNCGGGFAPRPIRPAKNWRGGNSLAKYPASTTVKHRPVDAAAHAAFAAAIEAVRPELR
                     "
     gene            639..1376
                     /locus_tag="AC16HB_15760"
     CDS             639..1376
                     /locus_tag="AC16HB_15760"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5608295.1"
                     /translation="MNAILLSLAAVFSTSAGGLCALRFGARLHLLLGFTAGALLGVVA
                     FDLLPEIFELSHRAGGDGLGAMVALAGGFLLIHLLETLVLVHTAHEADYARHHHPRVG
                     VLSAAAMIGHSFVDGVGIGLAFQVSPTVGVTVAIAVIAHDFCDGLNTVSLLLMHGNSR
                     SRARRMLAFNAVAPVLGALSTLAFAVSSASLTLFLGVFAGLLLYIGAADILPQAHSRA
                     GSAAAMGLIGCMVLGASCIYVVMRAAS"
     gene            complement(1435..>2207)
                     /locus_tag="AC16HB_15765"
     CDS             complement(1435..>2207)
                     /locus_tag="AC16HB_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5608296.1"
                     /translation="RGRRPSMHTGAATQECDVNTNQIARIAGMVGEPARTAMLIELMD
                     GRALTAAELARAARITPQTGSSHLAQLVDADLLRVTTSGRYRYYRLASPDIARMLESI
                     MQVASRAHGTAPRLVVGPKEASLRTARTCYDHLAGRLGVAIARRLALDGGLVLEDDDG
                     YLTDSGRVALGRLGVEVQADNAARPRSAAVFCRPCLDWSERRFHVAGRLGARICNHCF
                     EQGWIRRRQGSRAIDITPRGQAAMQTWLGMQLWRSVVP"
BASE COUNT          298 a          759 c          762 g          388 t
ORIGIN      
        1 caccgcggtg acatttcgca ggtctacgtc gatcccgcgc gacgaggcct cgcggtaggt
       61 tcgaagctca tgcggcggac gctggcgctg gcattcgaac tgcccggact cgagcaggtc
      121 gagctgaccg tcgtgtccac caacacggcc gcagtgcggc tctaccagcg actcggtttc
      181 gtgcagttcg gattgcagga gaattacttc aaggtcggtg agctgcgctg gaatatgtgc
      241 ctgatgcagc tctcacgcgc aagctatctc gccggcaatc gcccggcgtc caccgcaagg
      301 agtcagcatg cttgaaatga gacccacctg cgagaactgc aaccgggcgc tgccgcccga
      361 ttcgctcgag gcgcgcatct gctcgttcga gtgcacgttc tgcgccacgt gcgcgacggg
      421 cgttctgcgc aatgtctgcc ccaattgcgg cggcggtttc gcgccgcgtc cgattcgacc
      481 ggcgaagaac tggaggggcg gcaattccct cgccaagtat cccgcgagca cgaccgtcaa
      541 gcaccggccg gtcgacgcgg ccgcccacgc ggccttcgcg gccgcgatcg aggcggtgcg
      601 ccccgaactg agatgagacc gctgcgcgac ctgccgccat gaacgcgatc ctgctgtcgc
      661 tggccgcggt gttctccacg tccgcgggcg gcctgtgcgc gctgcggttc ggcgcgcgcc
      721 tgcacctgct gctgggcttc acggcgggtg cgctgctcgg cgtggtggcc ttcgatctgc
      781 tgccggagat cttcgaactg tcgcaccgcg ccggcggcga tggcctgggc gcgatggttg
      841 cgcttgccgg cggcttcctg ctgatccatc tgctcgaaac gctggtgctg gttcacaccg
      901 cgcacgaggc cgactacgcg cggcatcatc atccgcgcgt cggcgtgctg tcggcggcgg
      961 cgatgatcgg ccacagcttt gtcgatggcg tcggcatcgg cctcgcgttc caggtttcgc
     1021 cgacggtcgg cgtcaccgtc gcgatcgcgg tgatcgcgca cgacttctgc gacggcctga
     1081 acaccgtgag cctgctgctg atgcacggaa attcgcgctc acgcgcgcgc cgcatgctcg
     1141 cgttcaacgc cgttgcgccg gtcctcgggg cgctgtcgac gctcgcgttc gccgtgtcgt
     1201 ccgcgagcct gacgctgttt ctcggcgtct ttgccggcct cctgctgtac atcggcgcag
     1261 cggacatcct gccgcaggcg cactcccgcg ccgggtcggc cgcggcgatg ggtttgatcg
     1321 gctgcatggt ccttggcgcg tcgtgcatct acgtggtgat gcgcgcggca agctgatgct
     1381 gcggccgcag cggtacaccc gtggccatct ttcacctgcg gcgcagcctt gaggtcaggg
     1441 cacgacgctg cgccacagtt gcatccccag ccacgtctgc atcgccgcct ggccgcgcgg
     1501 cgtgatgtcg atcgcgcgcg aaccctgccg gcgccggatc cagccctgtt cgaagcagtg
     1561 gttgcagatg cgcgcaccga gccggccggc gacgtggaag cgacgttcgc tccagtccag
     1621 gcacggtcgg cagaacacgg ctgccgagcg cggccgcgct gcgttgtcgg cctgcacctc
     1681 gactccgagc cggccgagcg caacacgacc cgaatcggtc aggtagccgt cgtcgtcctc
     1741 gaggacgagt ccgccgtcga gcgcgagtcg acgcgcgatg gcgactccga gccggccggc
     1801 cagatggtca tagcaggtcc gtgccgttcg cagtgatgcc tctttcggtc cgacgaccag
     1861 gcgcggggcg gtgccatgcg cgcgcgaggc gacctgcatg atcgattcga gcatgcgggc
     1921 gatgtcgggc gaagcgagcc ggtaatagcg gtagcggccc gaggtcgtca cgcgcagcag
     1981 atccgcgtcg acgagttgcg ccagatggct gcttcccgtc tgcggggtga tgcgcgcggc
     2041 gcgcgcgagt tcggcggccg tcagcgcgcg cccgtccatc agttcgatga gcatggccgt
     2101 gcgcgccggc tcgcccacca tgccggcgat gcgcgcgatc tggttggtgt tcacgtcgca
     2161 ctcctgggtc gcggccccag tatgcatgct ggggcgccgc ccacgat
//
LOCUS       DBNHBY010000664         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1740597, whole genome shotgun sequence.
ACCESSION   DBNHBY010000664 DBNHBY010000000
VERSION     DBNHBY010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1740597"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="AC16HB_15770"
     CDS             complement(<1..1439)
                     /locus_tag="AC16HB_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5608297.1"
                     /translation="MKKRSTASPPPRRHPRIAIAALVVAVACTAAFAGSSWLRPLFGT
                     STVQSASDTQAAGSTSTTMTTRSAQSSSTTAEQTSTQRTATTSSAFTVVAWNDLGMHC
                     VDGKDYSIMSILPPYNNLHAHVINASTGKQVTSGVTLTYEAMSDPSGSRNTTSATKTN
                     FWSYVQALYGASPKPNYGLNLGDPATSNPTPGTTPAPMKFNSARGWFEAEGLPITPYD
                     DAGNKNFYPMVKVVARDSKGKQVAATQTVLPVSDEMSCKTCHASGSANNAAMPASGWV
                     NYLLDPEKDWKKNILRLHDDKHKSDPVYVAALARLGNAPTGLFDRATNGTPTLCSSCH
                     GSNALNSPGFTLTVNGAQVKVSALTSAMHSKHGKVIDPTKGVTLDAIGNRDSCYLCHP
                     GAVTKCLRGAMSEVPNLDCQSCHGNMTAVGQMSRVGWLQQPNCQACHHDGLREIAAVT
                     DVTTGALRQVTDTRFATNADAPAAGFSLFR"
     gene            complement(1698..>2695)
                     /locus_tag="AC16HB_15775"
     CDS             complement(1698..>2695)
                     /locus_tag="AC16HB_15775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5608298.1"
                     /translation="PGSAAYEVMRDLVNHLPVMVTPRWVRSKSSPIALANLLEYLVRV
                     AMLPKAAGRILDAGGPELLTYETMMRQFGAVVGRRPVIIPVPVLTPRLSSYWLALVTA
                     VPAPIARALIGGLKHDIPAHDDELRALVPQRLLTFKEAVVAALDAERTHTVAARWTEG
                     ALIFRGGRHDYAFYAKRASGSAVARAEPAAVWNVVTQIGGDTGYYYANALWRARAWID
                     WLVGGPGLSKGRRHPTELRVGDTIDYWTVLALEPERRLTLNFGMKAPGNGVMEFEIEP
                     LNERDTRVTITAYWHPAGVWGLLYWYALVPAHRFIFRGVTRAIARRAQAADARIR"
BASE COUNT          379 a          866 c          968 g          482 t
ORIGIN      
        1 cggaacagcg agaagccggc ggccggggcg tcggcgttgg tcgcgaagcg cgtgtcggtg
       61 acctggcgca gcgcgccggt cgtgacatcc gtgacggcgg cgatttcgcg caggccgtcg
      121 tggtggcagg cctggcagtt cggctgctgc agccagccga cacgcgacat ctggccgacc
      181 gcggtcatgt tgccgtggca gctctggcag tcgaggttcg gcacttcgct catcgcgccg
      241 cgcaggcact tggtgaccgc gcccggatga cataggtagc acgagtcgcg gttgccgatc
      301 gcatcgagcg tcacgccctt ggtcgggtcg atgaccttgc cgtgcttcga atgcatcgct
      361 gaagtcagcg cggagacctt cacctgagcg ccgttgacgg tcagcgtgaa gccgggcgag
      421 ttcagcgcgt tggagccgtg gcacgacgag cacagcgtcg gcgtgccgtt ggtcgcgcgg
      481 tcgaacagtc ccgtcggcgc attgccgagc ctggcgagcg cggcgacata gacaggatcg
      541 ctcttgtgct tgtcatcgtg caggcggagg atgttcttct tccagtcctt ctccgggtcg
      601 agcaggtagt tgacccagcc gctggccggc atcgcggcgt tgtttgcgga gccgctcgca
      661 tggcaggtct tgcagctcat ctcgtccgac acgggcagca ccgtctgcgt cgccgcgacc
      721 tgcttgccct tcgagtcacg cgccacgacc ttgaccatcg gatagaagtt cttgttgccg
      781 gcgtcgtcgt acggggtgat cggcaggccc tcggcctcga accagccgcg cgcggagttg
      841 aatttcatcg gcgccggcgt cgtgcccggc gtcgggttgc tggtcgccgg atcgccgagg
      901 ttcagtccgt agttcggctt cggtgacgcg ccgtacagcg cctgcacgta gctccagaag
      961 ttggtcttgg tcgccgacgt ggtgttgcgc gagcccgagg gatcggacat cgcctcgtag
     1021 gtgagcgtca cgcccgaggt cacctgcttg ccggtggacg cgttgatcac gtgcgcgtgc
     1081 aggttgttgt acggcggcag gatcgacatg atcgagtagt ccttgccgtc gacgcagtgc
     1141 atgccgaggt cgttccaggc gacgacggtg aatgcgctgc tggtcgtcgc cgtccgctgc
     1201 gtggacgttt gctcggcggt cgtgctgctc gactgcgcgc tgcgcgtggt catcgtggtc
     1261 gacgtcgagc cggccgcttg cgtatcgctg gcagactgca cggtcgaggt gccgaacagc
     1321 ggccgcagcc aggatgagcc ggcgaatgcg gcggtacagg cgacagcgac gacgagcgcc
     1381 gcgatggcta tgcgcgggtg tcgacgcggc ggcggactgg cggtgctgcg tttcttcatg
     1441 gtttggctcc ttgtgacgag aagacttgtt ctgcgacgcg cgcgggacgt gacggaccgc
     1501 gcgtgatcaa tgtcatgtcg atgatggggt cttcctttgc ggcggatcaa ggcgaaaggg
     1561 tcgtcctgat cgatgacgcg aagatgcgcg ctacctgcgc acggcatttc gcgccgcttg
     1621 cgtgatgcgc gtcacagacg acgtcgcggg cgttgcgctc gcaatgcgcg cgacgcggcg
     1681 ttcggtcgcg cgccggacta gcggatgcgc gcgtcggccg cttgcgcgcg gcgcgcgatc
     1741 gcgcgcgtga cgccacggaa gatgaagcgg tgcgcgggga cgagcgcgta ccagtacagc
     1801 aggccccaca cgccggccgg atgccagtag gcggtgatcg tgacgcgcgt gtcgcgctcg
     1861 ttcagcggtt cgatctcgaa ctccatcacg ccgttgcccg gcgccttcat gccgaagttc
     1921 agcgtgaggc gcctttcggg ctcgagcgcg agcacggtcc agtagtcgat cgtgtcgccg
     1981 acgcgcagct cggtcgggtg ccgccgccct ttcgacaatc cggggccgcc gacgagccaa
     2041 tcgatccatg cccgcgcgcg ccacagcgca ttggcgtagt agtagccggt gtcgccgccg
     2101 atctgggtga cgacattcca tacggcggcc ggctcggcgc gtgccactgc gctcccgctg
     2161 gcgcgtttgg cgtagaacgc gtagtcgtgc cggccgccgc ggaagatcag cgcgccttcg
     2221 gtccaacgcg ccgcgacggt gtgcgtcctt tccgcgtcga gcgccgcgac caccgcttcc
     2281 ttgaacgtca gcagccgctg cggtaccagc gcgcgcagtt cgtcgtcgtg cgcgggaatg
     2341 tcgtgcttca ggccgccgat cagcgcgcgc gcgatcggcg ccggaacggc ggtgacgagc
     2401 gcgagccagt acgacgacag ccgcggcgtc agcaccggca ccgggatgat caccggccgt
     2461 cgcccgacga cggcgccgaa ctgtcgcatc atcgtttcgt aggtcagaag ttcgggtccg
     2521 ccggcgtcta ggatccgccc ggccgccttt ggcagcattg ccacgcgcac caggtactcg
     2581 agcaggttgg cgagcgcgat cgggctcgac ttcgaccgca cccagcgcgg cgtgaccatc
     2641 accgggaggt ggttgacgag gtcgcgcatc acctcgtacg ccgccgagcc cgggc
//
LOCUS       DBNHBY010000665         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_387465, whole genome shotgun sequence.
ACCESSION   DBNHBY010000665 DBNHBY010000000
VERSION     DBNHBY010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_387465"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16HB_15780"
     CDS             <1..181
                     /locus_tag="AC16HB_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR5608299.1"
                     /translation="ARYAFNAPTIWAYDTTFMFYGAFFMLGAAFTLQRKGHVRTDSLY
                     ADWSPRTQAIVDLVG"
     gene            203..433
                     /locus_tag="AC16HB_15785"
     CDS             203..433
                     /locus_tag="AC16HB_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR5608300.1"
                     /translation="MAVFVFTGWGYFVKAYTIRETFVTSAWQPITWPFKLAMPLSGAL
                     LLLQGLSECLKCLHAIRAGRWPEVIHSTEGRA"
     gene            430..1740
                     /locus_tag="AC16HB_15790"
     CDS             430..1740
                     /locus_tag="AC16HB_15790"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5608301.1"
                     /translation="MSNEILGLAALGVLFAAIFIGFPIAFTLAALSLATGFIALGPVV
                     FDLAVLQTYSVMRDVVLASIPFFLFMGFLLEQSGLVERLFHGMQQLLAGLRGSLYLAV
                     LITATIFAAATGIVGSAVTLLGVMAGPSMIRSNYDVKLSAGAIAAGGTPGILIPPSVM
                     LIVMGPVVGVPATDLFAAAVLPGLLLSTIFTIWCLARCWINPSLGPPLPPELRPTSLG
                     PVVRDLALGVAPVIVVILSTLGVIMAGIATPTDAGAVGCAATFVLAFGSGRMNWAKLK
                     RAAYQTLEVSSMILLLVTASNLFGAVFSRLGSPEYLAQMLIGLQLPPLVMLALILALV
                     FVLGWPLEWVPIVLIVIPIVLPIVQAANIDLIWFCTLVAVTLQTAWLSPPVALSAYFL
                     KGVVPQWELKQIYAGMMQFMILQLVGVAIVMLFPKLATWLPSIN"
BASE COUNT          225 a          592 c          605 g          378 t
ORIGIN      
        1 cgcgcgctac gcattcaacg cgccgacgat ctgggcctac gacacgacgt tcatgttcta
       61 tggcgccttc ttcatgctcg gcgccgcgtt cacgttgcag cgcaaggggc acgtgcggac
      121 cgactcactg tacgcggact ggtcgccgcg cacgcaggcg atcgtcgatc tcgtcggcta
      181 attcatcttc tttctgcccc tggtggcggt attcgtgttc accggctggg gctatttcgt
      241 gaaggcctac acgatccgcg aaacgttcgt cacgagcgcc tggcaaccga tcacgtggcc
      301 gttcaagctc gcgatgccgc tgtcgggcgc gttgctgctg ctgcagggtc tctccgaatg
      361 cctgaagtgc ctgcacgcga tccgcgccgg gcgctggccc gaggtgattc attcgaccga
      421 gggccgcgcg tgagcaacga gatcctcggg ctggccgcgc tcggcgtgct gttcgcggcg
      481 atcttcatcg gcttcccgat cgctttcacg cttgctgcgc tgtcgctggc gaccggattc
      541 atcgcgctcg gcccggtcgt gttcgacctc gccgtgctgc agacgtactc ggtgatgcgc
      601 gacgtcgtgc tggcgtcgat cccgttcttc ctgttcatgg gtttcctgct cgagcagtcg
      661 gggctcgtcg agcgcctgtt ccatggcatg cagcaactgc tggccggtct gcgtggctcg
      721 ctgtatctcg ccgtgctgat cacggcgacg atcttcgccg ccgccaccgg catcgtcggc
      781 tccgcggtga cgctgctcgg cgtgatggcg gggccgtcga tgatccgcag caactacgac
      841 gtgaagctca gcgccggcgc gatcgctgcc ggcggcacgc ccggcatcct gatcccgccg
      901 agcgtgatgc tgattgtgat ggggccggtg gtcggcgtgc cggcgacgga tctcttcgca
      961 gcggcggtgc tgccgggtct gctgctgtcg acgatcttca ccatctggtg cctggcgcgc
     1021 tgctggatca acccgtcgct cggtccgccg ttgccgccgg aactgaggcc gacatcgctc
     1081 ggcccggtcg tgcgcgacct cgcgctcggc gtggcgccgg tgatcgtggt gatcctgtcc
     1141 acgctcggcg tgatcatggc cggcatcgcc acgccgaccg acgccggcgc ggtcggctgt
     1201 gccgcgacct tcgtgcttgc gttcggctcg ggccgcatga actgggccaa gctcaagcgc
     1261 gctgcgtacc agacgctcga agtgtccagc atgatcctgc tgctggtgac ggcgtcgaac
     1321 ctgttcggcg cggttttctc gcgtctcggc agccccgagt acctggcgca gatgctgatc
     1381 ggcctgcagc tgccgccgct ggtgatgctg gcactgatcc tggcgctcgt gttcgtgctc
     1441 ggctggccgc tcgagtgggt gccgatcgtg ctgatcgtga tcccgatcgt gctgccgatc
     1501 gtgcaggcgg cgaacatcga cctgatctgg ttctgcacgc tggtcgcggt caccctgcag
     1561 accgcgtggc tgtcgccacc ggtggcgctg tcggcgtact ttctcaaggg cgtcgtgccg
     1621 cagtgggagc tgaagcagat ctatgcgggc atgatgcagt tcatgatcct gcagctggtc
     1681 ggcgtggcca tcgtgatgct gttcccaaag ctcgcgacgt ggctgccgag catcaactag
     1741 acatgcgggg aaacccgcgt tggtggcgcg tcgcatcatc ggcgcgtcac gcatccgagg
//
LOCUS       DBNHBY010000666         5661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_522858, whole genome shotgun sequence.
ACCESSION   DBNHBY010000666 DBNHBY010000000
VERSION     DBNHBY010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_522858"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="AC16HB_15795"
     CDS             <1..325
                     /locus_tag="AC16HB_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5608302.1"
                     /translation="RSLAPRFQDGLRALAASPIVGEVRGAGLIAAVEFVKNRQTRESF
                     SPTVTVAAHFTERAQAHGLLVRQLGEAVALCPPLIIEPREIDELLARFTRALRDTEQY
                     AHQYG"
     gene            345..1082
                     /locus_tag="AC16HB_15800"
     CDS             345..1082
                     /locus_tag="AC16HB_15800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608303.1"
                     /translation="MPDRKTLLLTGASRGIGHATVKHFTAAGWRVLSVSRQPWTPDCP
                     WGEGAENHIQMDLASVADIERGIGEIRARLKDGRIEALVNNAGISPKEDGGGRLGVRQ
                     TDYATWLQVFNVNLFSVALLARGLFDELRAGAATIVNVTSIAGSRVHPFAGVAYATSK
                     AALAALTRELAHEFGPYGIRANAIAPGEIDTSMLSPGTDEIVREQIPLQRLGKPEEVA
                     ATIYFLCTGGASYINGAEIHIDGGQQT"
     gene            1290..2558
                     /gene="gabT"
                     /locus_tag="AC16HB_15805"
     CDS             1290..2558
                     /gene="gabT"
                     /locus_tag="AC16HB_15805"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - GABA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HIR5608304.1"
                     /translation="MTAPKDIEQRRLAASPRGVGVMAGFYATRAENATLWDDEGRAYT
                     DFAAGIAVLNTGHRHPKVVAAIRDQLDRFTHTAYQIVPYESAIALMERINAVAPIAGT
                     KKTALFTTGAEAVENAVKIARAYTKRAGVIAFSGAFHGRTLMGLALTGKVDPYKAGFG
                     PFPPEIYHLPFPHALHGVSVADSMAALETLFKSDVEPQRIAAVIVEPVQGEGGFNIAP
                     PELLVRLREVCDRHGIVLIADEVQTGFGRTGKLFAMEHHSVQPDLMAIAKSLGGGMPL
                     SAVCGRAEIMDAPAPGGLGGTYAGNPLAVAAAHAVLDVIQEERLCERAVSLGRQLTER
                     LQAARSRCPALAEVRGLGSMVAAEFVDPANGRPSADHAKRVQQRAMQGGVVLLTCGTY
                     GNVIRFLYPLTIPQAQFDAALDTVTRALAD"
     gene            2609..3697
                     /locus_tag="AC16HB_15810"
     CDS             2609..3697
                     /locus_tag="AC16HB_15810"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608305.1"
                     /translation="MNDKFVEFDDVCKTYDGAANVVDHLHLAIAEGEFLSLLGPSGSG
                     KTTTLMMLAGFERPTAGHIRLRGKPIDSAPPYKRNFGMVFQNYALFPHMTVAENLAFP
                     LSVRKIAAADVQARVKRALDMVRLPAMAQRMPGQLSGGQQQRVAVARALVFEPQLILM
                     DEPLGALDKQLREEMQLELRGLHDRLGITMVYVTHDQSEALTMSDRIAVFHQGKIQQI
                     ADPQTIYERPSNLFVASFIGESNRLVGDIRATDPAGGCVAEVTGGARLRGRAAAECAA
                     SARATISVRPERIMVCMPGAQAPDANVLAGQVEETIFLGNHVRVRLKCAAGEVVANVP
                     IDQAGTIPGAGHPAVAHWPTASTVIFPQ"
     gene            3731..4783
                     /locus_tag="AC16HB_15815"
     CDS             3731..4783
                     /locus_tag="AC16HB_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608306.1"
                     /translation="MSFGTKAMWAALGAALAMASGAAGAQGRDLSVVSWGGAYQDAQK
                     EVYFKPFMEKTKIKMVDESWDGGIGVLRAKLQGGANNWDVVQVESEELALGCEEGLFE
                     KLDWAKLGGRDKFLKDAVSECGVGAIVYNFVLAYDGDKFKDGPKSWKDFWDVKKFPGK
                     RALRKGPKTNLEIALIADGVAPADVYKTLSTPAGVERAFNKLAQLKANLIWWEKGSQP
                     PQMLASGEAAMVSAYNGRIAAANKTDKKNFKMSWPGSLYTIDSWVIMKGSPNKAKAEQ
                     FLAFASDPQNQKNLPKYISYGITNNAATALVDKALLPDLPTNPDHLKSALYINDKFWL
                     ENLDKLNQRFNAWLAK"
     gene            4877..>5661
                     /locus_tag="AC16HB_15820"
     CDS             4877..>5661
                     /locus_tag="AC16HB_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608307.1"
                     /translation="MARAARLHALSLARQLTRAERAKKLKAALLTAPLFVFLVISFLG
                     PIGALLTKSFVDPDLAVAMPNVSREIKRWDGRASPDEPTFAALIADVRAARAAGTLAP
                     VATRLNYDISGFRTLLFATGRALPDALEQSARDTLLAIDPKWGEPETWGAIARAAGPT
                     TSLFLLAAVDLKRDAAGGLVGAPQEEAIFVQVLGRTFAIATAVTILCLLLGYPLAYAL
                     AQAPTRVANVLLIFVLLPFWTSLLVRTSAGIVLLQHEGVINEAL"
BASE COUNT          978 a         1868 c         1912 g          903 t
ORIGIN      
        1 gcgctcgctg gcgccgcgct tccaggacgg actgcgcgcg ctggctgcca gtccgatcgt
       61 tggcgaagtg cgcggcgcgg gactcatcgc ggccgtcgag ttcgtgaaga acaggcaaac
      121 cagggaatcg ttttcgccca ccgtaaccgt ggctgcacac tttaccgagc gtgcgcaggc
      181 gcatgggctg ctcgtgcgcc agctcgggga ggcggtcgcg ctctgcccgc cgctcatcat
      241 cgaaccgcgc gagatcgacg agttgctggc gcgcttcacg cgcgccctgc gcgacactga
      301 acagtacgcg catcagtacg gctgatcgaa tgacgcgctg acccatgccc gatcgcaaga
      361 cgctcctgct gaccggtgcc agccgcggca tcggccatgc cacggtcaag cacttcaccg
      421 cggcgggttg gcgcgtcctg tcggtttcgc gccaaccgtg gacgccggac tgcccgtggg
      481 gagagggcgc ggaaaaccac atccagatgg atctcgccag cgtcgctgac attgagcgcg
      541 ggatcggtga gatccgcgca cgtctgaaag acggccgcat cgaagcgctg gtcaacaacg
      601 ccggcatctc gccgaaggaa gacggcggcg gccggctcgg cgtgcggcaa accgattacg
      661 cgacctggct tcaggtgttc aacgtcaatc tcttctccgt ggcgctgctc gcgcgcgggc
      721 tgttcgacga gttgcgcgcg ggtgcggcga cgatcgtcaa cgtgacctcg attgccggca
      781 gccgggtcca tccgttcgcc ggcgtcgcct atgcgacgtc taaggccgcg cttgccgcgc
      841 tcacgcgcga actggcgcac gagttcggac cgtacggcat ccgtgccaat gccatcgcgc
      901 cgggggagat cgacacgtcg atgctgtcgc cgggcaccga cgagatcgtg cgcgaacaga
      961 tcccgctgca acggctcggc aagccggagg aggtcgccgc cacgatctac tttctgtgca
     1021 ccggcggcgc gtcgtatatc aacggcgcgg agatccacat cgacggcggg cagcaaacat
     1081 gaggtcggaa accctgcgtt gcgaccgacg tcggcgagct gccggcgcgt caggaagccg
     1141 ggccgccatc ggcctctgga tgaaccgttt gcgcgccgcg ggcgatctgg cacgatccga
     1201 tggcacaccg tggtgctgga ctacgcttcc gcggaacaag tgatttctgt ccagcccatc
     1261 ttccttcgat tcacgagcag agggacgaca tgaccgcacc caaggacatc gagcaacgtc
     1321 gactcgccgc ctccccccgt ggcgtcggcg tcatggccgg cttctatgcc acgcgggccg
     1381 agaatgcgac cctgtgggac gacgaaggcc gcgcgtacac ggacttcgcg gccggcattg
     1441 ccgtcttgaa caccggccat cggcatccga aagtcgtggc ggcgatccgt gaccagctcg
     1501 accgcttcac ccacaccgcc taccagatcg tgccgtacga aagcgccatc gctctgatgg
     1561 agcgcatcaa tgccgttgcg ccgatcgccg gaaccaagaa gaccgctttg ttcaccaccg
     1621 gggccgaagc ggtggagaac gcggtcaaga tcgcgcgcgc ctacacgaag cgcgccggtg
     1681 tcatcgcttt cagcggcgct ttccacggtc gcacgctgat gggtctggcg ttgacgggga
     1741 aggtcgatcc ctacaaggcc ggcttcggac cgtttccgcc ggagatctac caccttcctt
     1801 tcccgcatgc gctgcacggc gtgtcggttg ccgacagcat ggcggcgctg gagacgctgt
     1861 tcaagtccga tgtcgagccg cagcgcatcg ccgccgtcat cgtcgaaccc gtgcagggcg
     1921 aaggcgggtt caacattgcg ccacccgaac ttctggtgcg cctgcgcgaa gtctgcgacc
     1981 gtcacggcat cgtgctgatc gccgacgaag ttcagaccgg tttcggacgc accggcaagc
     2041 tgttcgccat ggagcatcat tcggtccagc ccgacctgat ggccatcgcc aagagcctcg
     2101 gcggcggcat gcccctgtcg gccgtctgcg gacgtgccga gatcatggat gcgcccgcgc
     2161 cgggcgggct gggcggaacg tacgcgggca accccttggc tgtcgctgcg gcgcacgcgg
     2221 tgctcgacgt gatccaggaa gagcggttgt gcgagcgtgc tgtcagcctc ggccgacagt
     2281 tgacggagcg gctgcaggcg gcgcgcagcc gctgcccggc gctggccgaa gttcgcgggc
     2341 tgggctcgat ggtggcggcc gagttcgtcg atcccgccaa cggccgtccg agcgccgacc
     2401 acgccaagcg cgtgcagcag cgtgcgatgc aggggggcgt ggtgttgcta acctgcggca
     2461 cgtatggcaa cgtgattcgc ttcctgtatc cgctgacgat tccgcaggcg cagttcgatg
     2521 ccgcattgga tacagtgaca cgggcgctcg ctgactgaag cgtccgcagc cgtgaggaaa
     2581 tcgtcgcgac cgaccgcgcg cggcaccgat gaacgataaa ttcgtcgagt tcgacgacgt
     2641 ctgcaagacc tacgacggcg cggccaacgt cgtcgaccac ttgcacctcg ccatcgccga
     2701 aggcgagttc ctgtcgctgc tcggcccgag cggatcgggc aagacgacga cattgatgat
     2761 gctggccgga ttcgaacggc ccaccgccgg ccacatccgg ctgcggggca agccgatcga
     2821 ctcggcacca ccatacaagc gcaatttcgg catggtgttc cagaactacg cgctgtttcc
     2881 gcacatgacg gtggcggaga accttgcctt cccgctgtcg gtgcgcaaga tcgctgccgc
     2941 cgacgtccag gcgcgcgtga agcgcgcgct cgacatggtg cggctgcccg cgatggcgca
     3001 gcgcatgccc gggcaactgt cgggtggcca gcagcagcgt gtcgcggtgg cgcgggcgct
     3061 ggtgttcgag ccccagctga tcctgatgga cgagccgctc ggcgcgctcg acaagcagtt
     3121 gcgcgaggaa atgcagctgg agttgcgcgg tctgcacgac cgcctcggca tcacgatggt
     3181 gtatgtcacg cacgaccagt ccgaagcgct gacgatgtcc gaccgcattg cggtgtttca
     3241 tcaaggcaag attcagcaga tcgctgaccc ccagacgatc tacgaacgtc cttcgaacct
     3301 gttcgtggcc tcgttcattg gcgagagcaa ccgcctggtc ggggacatcc gcgccaccga
     3361 tcccgccggc gggtgcgtcg ccgaagtcac gggcggcgcc cgcttgcgcg gccgcgccgc
     3421 ggcagaatgc gccgcgtctg cgcgggccac gatttcggtg cgtccggaac gcatcatggt
     3481 gtgcatgccg ggtgcgcagg caccggacgc caacgtgctg gccggccagg tcgaggaaac
     3541 gattttcctc ggcaatcacg tgcgcgtgcg cctgaaatgc gccgccggcg aagtcgtcgc
     3601 gaacgtgccg atcgatcagg ccggcacgat ccccggtgca ggccacccgg ccgtcgcgca
     3661 ctggcctaca gcgagtacag tcatctttcc gcagtgagtg agtcgtcaac ctcgacatgg
     3721 gaggagagca atgtcatttg gaacgaaggc gatgtgggca gcactcggtg cagcgctggc
     3781 gatggcaagc ggcgctgccg gcgcccaggg gcgtgacctg tcggtcgtgt cgtggggcgg
     3841 cgcctaccag gacgcgcaga aggaggtcta cttcaagccg ttcatggaga agaccaagat
     3901 caagatggtc gacgaaagct gggacggcgg catcggcgtg ctgcgcgcca agctgcaggg
     3961 gggcgccaac aactgggacg tggtccaggt cgagagcgag gagctggcac tcggctgcga
     4021 ggaagggctg ttcgaaaagc tcgactgggc caagctcggc ggccgcgaca agttcctgaa
     4081 ggacgcggtc agcgagtgcg gcgtcggcgc gatcgtctac aacttcgtgc tcgcgtacga
     4141 cggcgacaag ttcaaggacg gcccgaagag ctggaaggac ttctgggacg tgaagaaatt
     4201 ccccggcaag cgcgcgctgc gcaaggggcc gaagactaac ctcgagatcg cgctgatcgc
     4261 cgacggcgtc gcgccggccg acgtgtacaa gacgctttcg accccggctg gcgtggagcg
     4321 cgcgttcaac aagctcgcgc agctgaaggc gaacctgatc tggtgggaga agggctcgca
     4381 gccgccgcag atgctggcgt cgggcgaggc cgcgatggtc tccgcgtaca acggccgcat
     4441 tgccgccgcc aacaagaccg acaagaagaa cttcaagatg agctggccgg ggagtctcta
     4501 cacgatcgac tcgtgggtga tcatgaaggg ctcgcccaac aaggcgaagg ccgagcagtt
     4561 cctcgcgttc gccagcgacc cgcagaacca gaagaacctg cccaagtaca tctcttacgg
     4621 catcaccaac aacgccgcga cggcgctggt cgacaaggcg ctgctacccg acctcccgac
     4681 caatccggat catttgaagt cggcgctcta catcaacgac aagttctggc tcgagaacct
     4741 cgacaagctg aaccagcggt tcaacgcctg gctcgccaaa taggcggttc aggaccgtag
     4801 gccggaatcg cctcacgcat ccgggccgac ggcggcaagc gccgctacgg ctcgctgcac
     4861 ggggcgaacc ccgtacatgg cacgcgcggc ccggctccac gcgctgtcgc tcgcgcggca
     4921 gctgacgcgc gcggaacgcg cgaagaagct caaggcggcg ctgctcaccg cgccgctttt
     4981 cgtttttctg gtgatcagtt tcctcggacc gatcggcgcg ctgctgacca agagcttcgt
     5041 cgaccccgat ctcgcggttg cgatgccgaa cgtcagccgc gagatcaagc gctgggacgg
     5101 gcgcgcatcg cccgacgagc cgaccttcgc cgcactgatc gcggacgtgc gcgcggcgcg
     5161 cgcggccggc accctggcgc cggtggccac gcgcttgaac tacgacatca gcggctttcg
     5221 cacgctgctg tttgcgaccg gtcgcgcgct gccggatgcg ctcgagcaat cggcgcggga
     5281 cacgctgctc gcgatcgatc ccaaatgggg cgagcccgag acctggggcg cgatcgcgcg
     5341 cgcggccggg ccgacgacgt cgctgttcct gctcgcggcg gtcgacctca agcgcgacgc
     5401 cgccggcggt ctggtcggcg cgccgcaaga ggaggcgatt ttcgtacagg tgctcggccg
     5461 cacgttcgcg atcgcgaccg cggtgacgat cctgtgcctg ctgctcggct acccgctcgc
     5521 ctacgcactg gcgcaggcgc cgacgcgcgt ggccaacgtg ctgttgatct tcgtgctgct
     5581 gccgttctgg acctcgctgc tcgtgcgcac gtcggcgggg atcgtgctgc tgcagcacga
     5641 gggcgtgatc aacgaggcac t
//
LOCUS       DBNHBY010000667        14800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_48873, whole genome shotgun sequence.
ACCESSION   DBNHBY010000667 DBNHBY010000000
VERSION     DBNHBY010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_48873"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16HB_15825"
     CDS             <1..234
                     /locus_tag="AC16HB_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427758.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5608308.1"
                     /translation="AGTLVVAGGVGANVQLRERLSDETRRIGATVYFPPPDLCTDNGA
                     MIAVAGLARLRRGAFDGSGAFTVHPRWTLSDAR"
     gene            complement(231..881)
                     /gene="plsY"
                     /locus_tag="AC16HB_15830"
     CDS             complement(231..881)
                     /gene="plsY"
                     /locus_tag="AC16HB_15830"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR5608309.1"
                     /translation="MESLWVWLANLAAIAAAYLLGSVPFAVLVSRLMGLADPRTYGSR
                     NPGATNVLRSGSSFAAALTLVGDAAKGAVAVWAARRFGAPFGVGDWGVAFAGLAAFIG
                     HLYPVFLRFTGGKGVATFLGVLLTLTPWVGVLACLVWVLIAVFFRYSSLSSISAAIVA
                     PLAQLMGWGFEPVLVASIVMSGLLIMRHQKNIAQLLAGKERKIGEKAVVAVQQRRH"
     gene            complement(881..1375)
                     /locus_tag="AC16HB_15835"
     CDS             complement(881..1375)
                     /locus_tag="AC16HB_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR5608310.1"
                     /translation="MAKRAHVSETPATQWLKQHRVPYTEHVYEYIEHGGTAASSRALG
                     VLEHHVVKTLVMEDEAGSPLIVLMHGDRTVSTRNLARQIGRKRIEACRPDVAQRHTGY
                     LVGGTSPFNTRKALPLFVERSILDLPRIFINGGRRGFLVGLDPRVLTDPLGAQPVDCA
                     LGES"
     gene            complement(1396..2295)
                     /gene="xerD"
                     /locus_tag="AC16HB_15840"
     CDS             complement(1396..2295)
                     /gene="xerD"
                     /locus_tag="AC16HB_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR5608311.1"
                     /translation="MTGAGSDPNLDRFLDAVWLEDGLAANTLAAYRRDLEGLAAWLAG
                     HDGATLVTATDVQLTGYFAATFAASRSSTANRRLASMRRFYRWALRERMIEADPTLQL
                     APAKRPQRFPAAISEAQVEALLAAPQREMPLGLRDRAMLELLYATGVRVSELVGLKLI
                     ELSLNDGLVRVLGKGSKERVVPLGEEARAWLQRYLDEARPALLAGRGADALFVTRRAR
                     AMTRQRFWGLIKRYARTAGIHAPLSPHGLRHAFATHLLNHGADLRVVQMLLGHADIST
                     TQIYTHVARERLQALHARHHPRG"
     gene            complement(2292..2864)
                     /locus_tag="AC16HB_15845"
     CDS             complement(2292..2864)
                     /locus_tag="AC16HB_15845"
                     /inference="COORDINATES: protein motif:HMM:NF027363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR5608312.1"
                     /translation="MKRWIAALALAAVASGVGAQPVEIEGVKFEPTVQVGGQALQLNG
                     AGLRTRAFFKVYAAGLYVPQKANSAAALLAQKGPRRVEIHMLRDISADTFAEALMEGL
                     KANHTEAQLAGWKAQIDALQSNMKSIGEAKKGSVIFFEFTPEAGTRIVVNGEPKGNSI
                     AGEEFFTAVLRVWIGDKPADAGLKKGLVGA"
     gene            complement(2887..3831)
                     /gene="ispH"
                     /locus_tag="AC16HB_15850"
                     /gene_synonym="lytB"
     CDS             complement(2887..3831)
                     /gene="ispH"
                     /locus_tag="AC16HB_15850"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5608313.1"
                     /translation="MEVLLAQPRGFCAGVDRAIEIVERALRIFGAPIYVRHEIVHNTF
                     VVESLRGKGAVFVDELDQVPDGATVIFSAHGVAKTVHAAASARGLRVFDATCPLVTKV
                     HVEVEKMRAQGREIVMIGHTGHPEVEGTMGQVDDGMYLVETVADVERLAVRDPDRLAY
                     VTQTTLSIDDAADVVSALKQRFPAIAEPKKADICYATQNRQDAVKFMAPQVDLVIVIG
                     SPTSSNSNRLREVAERFGVPAYLVDSAGDVQPHWLSGAARVGVTAGASAPEELVQQLT
                     ARLKSLGAGSVRTLQGVQENVVFPLPKGLGKSVTPEAA"
     gene            complement(3831..4280)
                     /locus_tag="AC16HB_15855"
     CDS             complement(3831..4280)
                     /locus_tag="AC16HB_15855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR5608314.1"
                     /translation="MTVIAPGAHVTLHYRLAVRVGDREHEIVSTMDSRPATLQIGAGQ
                     LAPALEERLVGLEEGARATFDLAPGLAFGPRNPELVQSLTRAMFDRHTDPGAAHAVGE
                     MLEFDAPNGGRMAGVLKQVDQDRVVIDFNHPLAGQPVRFSVQVIGVL"
     gene            4325..4999
                     /gene="radC"
                     /locus_tag="AC16HB_15860"
     CDS             4325..4999
                     /gene="radC"
                     /locus_tag="AC16HB_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR5608315.1"
                     /translation="MSLKNWPASERPRERLLAQGPGALSEAELLAVFLRTGVRGQTVL
                     DLARRALDHFGGLNGLLSAPQGDLQRIAGFGPAKCAQLHAVLELARRAVREEVRRDTL
                     LNTPARVREYLTLSLRNERHEVFVALYLDSQNRLIVDEVLFRGTLSQTSVFPREVVKR
                     ALHHNAGAVIFAHNHPSGLAEPSRADELLTRALKDALAVVDVRVLDHLIVAGHASVSF
                     AERGLL"
     gene            5101..5334
                     /gene="rpmB"
                     /locus_tag="AC16HB_15865"
     CDS             5101..5334
                     /gene="rpmB"
                     /locus_tag="AC16HB_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR5608316.1"
                     /translation="MARVCQVTGKKPMTGNNVSHANNRTKRRFMPNLQNRRFWVESEK
                     RWVRLRLTNAGLRTIDKLGIDAVLADLRARGEI"
     gene            5346..5513
                     /gene="rpmG"
                     /locus_tag="AC16HB_15870"
     CDS             5346..5513
                     /gene="rpmG"
                     /locus_tag="AC16HB_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR5608317.1"
                     /translation="MAKGAREKIKLESTAGTGHFYTTTKNKRTTPEKLEISKFDPVAR
                     KHVMYKESKLK"
     gene            complement(5582..6787)
                     /locus_tag="AC16HB_15875"
     CDS             complement(5582..6787)
                     /locus_tag="AC16HB_15875"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR5608318.1"
                     /translation="MQLVEFLDGGLLDLTWWQLIVAALALTHVTIAAVTIFLHRSQAH
                     RGLDLHPIVAHFFRLWLWFTTGMQTKEWVAIHRKHHAKCETPDDPHSPQTRGLKTVLW
                     TGAELYMTEAKNKETLARYGHGTPNDWLERNVYSRFTWQGCAVMLVADVLLFGAAGIA
                     VWAVQMIWIPLFAAGVINGIGHFWGYRNFDSPDASSNISPWGILIGGEELHNNHHTYP
                     TSARLSNKWYEFDLGWLYIRILSALGLARILRVAPVARVAAPRAPVDVQTLQAVISNR
                     YDLLAKYARGLQKTLRDELDKLRLPTEERARFARLTRWLRKGDVGQIPATQQQTLRDL
                     LTRSRVLQTLIEMRADLAVTWERSSVSREQMLAHLQDWIARAEVSGIRVLQDAALRIR
                     SYTTNAIPV"
     gene            complement(6891..8228)
                     /locus_tag="AC16HB_15880"
     CDS             complement(6891..8228)
                     /locus_tag="AC16HB_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5608319.1"
                     /translation="MTEAPLQELLQALAEGLARPAHFWSAIAIGCAIIGGWFASRIVR
                     QRVEARLAQRIAAGGTQADALRFSIEGFRRLAFPIAAQLLLWAGEGLLRVTGRLPHPG
                     DVRLLRLAMTLLGAMALVRLIVYVLRRVFTRAAFLGVFERSIAAVVWVVVALHLVGAL
                     DDVVQWLDAHTLPLGRTPVSLWTLVTGTISVLVTLLVALWIGSALEARLLRAETVDMN
                     VRVVVARLVRALLLTVGVLLALSLAGIDLTVLSVFGGALGVGLGLGLQRIASNYVSGF
                     IILLDRSLRIGDLITVEKYYGVVSQIRTRYTVVRALDGTEAIVPNEMLVSSPVTNHSY
                     TDRRVRVSVKVAVAYDSDLQRALALMEEAARSTARVLVDPAPSAVLLGFGADGLDLEV
                     GFWIADPEQGRMNVQSLVAQEILARFRTAGIAIPFPQREVRISGWNPGLGGPG"
     gene            complement(8225..9517)
                     /locus_tag="AC16HB_15885"
     CDS             complement(8225..9517)
                     /locus_tag="AC16HB_15885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR5608320.1"
                     /translation="MNGVRVTSQVVDGLAHLLGQVLRFDGPADAVMSRYFKQQRTLGA
                     RDRSLIAEATFFVLRRYATLTAQLAPAHPARAPRLAALLALARQHGIDALGPRALRSD
                     ERAVRHALAMDLAHLPGAMRAEVPTWLFAHVTAQYHDAGPLFAALAEPAPLTLRVNTI
                     KAQRDDVLGQLHKTTREHAPLAADPTRYAPDGIRLAEKPALTRWPLYQDGLIEVQDEG
                     SQLIARLVAPKRSDMVVDFCAGAGGKTLALGSLMRSSGRIYAFDIHAKRLAGLGPRLK
                     RSGLSNVHPAAIASENDARVKRLAGKIDRVLVDAPCSGSGTLRRNPDLKWRFDEAELA
                     RVNQVQGNLLRAASRLVKPGGRLVYSTCSLLGLENQQVVERFLAEHGEFSIVPAADVL
                     AAQKIEVDHAARFAPWFVMLPHLHDTDGFFAAVLERKR"
     gene            complement(9514..10164)
                     /gene="purN"
                     /locus_tag="AC16HB_15890"
     CDS             complement(9514..10164)
                     /gene="purN"
                     /locus_tag="AC16HB_15890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR5608321.1"
                     /translation="MRNIVCLISGRGSNLGAILRSARDEDWEQSFAARIACVISNRAD
                     AAGLEFARNAGVRTRVIPHGDYASRDAFEAALADAIDVHDPVLVVLAGFMRVLTARFV
                     DRYAGRLINIHPSLLPVFPGLHTHRQALAAGVKVHGCTVHFVSNEVDGGPIIAQAAVK
                     VRADDTEVALAARVLAQEHRLLPRCVRWVCEGRIRLDGDHVAARDLDADAQSVLAP"
     gene            10262..11197
                     /locus_tag="AC16HB_15895"
     CDS             10262..11197
                     /locus_tag="AC16HB_15895"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR5608322.1"
                     /translation="MKIFRGLPPPSQRHPCALAIGNFDGVHRGHRALLTRVVQAARQR
                     GLISAVMTFEPHPRELFAPDKAPARVANLRDKLEGLKTCGIDHVYLQHFNRRFASVGA
                     ADFIDRMLVERCRMKWLLVGADFRFGAHRAGDLALLRAHAARGGYEVEQLETVTNDGE
                     RVSSSAVRAALARGDLARAAHLLGHPYAITGRVLHGAKRGRTLGYPTLNLRIAHKRPA
                     AQGIYAVCVHGIGDAPWPGVASVGVRPTVEDSGRWLLEVHLFDFADQIYGRLIRVEFI
                     KKLRDETKYDTLDALTTAIRNDADQARALLLSSGI"
     gene            11275..14142
                     /gene="ileS"
                     /locus_tag="AC16HB_15900"
     CDS             11275..14142
                     /gene="ileS"
                     /locus_tag="AC16HB_15900"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR5608323.1"
                     /translation="MKPPADPASKKYPLNLPDTPFPMRGDLPKREPQWVQEWDARKVY
                     QAIRSARKGAPKFILHDGPPYANSEIHLGTAVNKILKDIVVKTRSMAGFDAVYVPGWD
                     CHGMPIEIHIEKKFGKSLPRAEVQAKARAHATEQIALQMQGFKRLGVLGDWDNPYKTM
                     TFKAEADEIRTLGRMIERGFVYRGLKPVNWCFDCNSALAEAEVEYIDKKTPVIDVAFP
                     LAKADEPKVAAAFGVAALGKPTATVIWTTTPWTIPANQALNFHPDFIYALVDCGDRLL
                     ILAESLVESCLQRYGLKGRVLATCIGKALELVRFRHPLAPLDKGYDRDSPVYLGTYVT
                     ADAGTGIVHSSPAYGVEDFDSCKSYGMTNDQILNPVTGDGTYAKDFPLFGGLRIWDAN
                     PKVVDALRVAGALLHASADTHSYMHCWRHKTPLIYRAASQWFVRMDGATRETQGVMQR
                     ETVGETLRQTALRAIEQTAFYPAWGKARLHGMIANRPDWCISRQRNWGVPLPFFLHRE
                     TGELHPRTLELLEVAAQKVEQGGVEAWSRATVEDFLSPAEAMQYTKINDILDVWFDSG
                     STHRTVMRGSHASELAYPADMYLEGSDQHRGWFHSSLLTGAAIDGRAPYKALLTHGFV
                     VDALGRKMSKSLGNGIDPRELADQMGAEIMRLWVASTDYSGEMSIGDEILKRVVESYR
                     RIRNTLRFLLANTSDFDVARDAVPIGELVEIDRYAIALTAQMQAEAQANCDRFEFHPV
                     IARLQIFCSEDLGAFYLDILKDRLYTTAPDSRARRSAQTALWHITASLLRLMAPYLSF
                     TAEEAYWVFVRDADATIFTDVYHELPPVPGHEALLSKWAQIRAARAEVLKQIEALREA
                     GKIGSSLQAEADLHASGERHALLATLDDDLKFVLITSRATLHAAAKPADEKIIVTPTA
                     QRKCDRCWHWRADVGGHAEHPTLCGRCVTNLFGAGEPRRAA"
     gene            14142..14642
                     /gene="lspA"
                     /locus_tag="AC16HB_15905"
     CDS             14142..14642
                     /gene="lspA"
                     /locus_tag="AC16HB_15905"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR5608324.1"
                     /translation="MAWSGGSTWRQRLVPWLGLSTVIVLADQLTKIVIERKFEFGDVH
                     PVTGFFNLVLTYNKGAAFSFLANASGWQSRFFTAVGIAASLFIVYLLARHGAQRLFAL
                     SLALILGGAVGNVIDRLVHGHVIDFLDFHWRGWHWPAFNLADSAIVGGAALLILDELL
                     RVRKSK"
     gene            14653..>14800
                     /locus_tag="AC16HB_15910"
     CDS             14653..>14800
                     /locus_tag="AC16HB_15910"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR5608325.1"
                     /translation="MDLDGKRIVLGMTGGIAAYKVCELARRLQDDGADVQVVMTRGAV
                     QFVTA"
BASE COUNT         2566 a         5091 c         4818 g         2325 t
ORIGIN      
        1 gccggcacgc tggtggtggc aggcggcgtc ggcgccaatg tgcagttgcg cgagcggttg
       61 agcgatgaaa cgcgccgcat cggcgcaacc gtctactttc cgccgcctga cctgtgcacc
      121 gacaatggcg caatgatcgc cgtcgcgggg ctcgctcgcc tgaggcgcgg cgcgttcgac
      181 ggaagcggcg ccttcaccgt gcacccacgc tggacgcttt ccgacgcacg ctagtgccgg
      241 cgctgttgca cggcaaccac cgccttctcg ccgatcttgc gttccttgcc cgccagcagc
      301 tgggcgatgt tcttctggtg ccgcatgatc agcagcccac tcatcacgat cgaagcgacc
      361 agcaccggtt caaaccccca tcccatcagt tgcgccagcg gcgcgacgat cgcggcgctg
      421 atcgacgaca gcgacgagta gcggaagaac accgctatca gcacccagac gaggcatgcc
      481 agcactccga cccagggcgt gagtgtcagc agcacgccga ggaaggtcgc gacccccttg
      541 ccgccggtga agcgcagaaa gaccggatac aggtgaccga tgaacgccgc gagacccgcg
      601 aacgcgacac cccagtcacc gacaccgaac ggcgcaccga agcggcgcgc ggcccagaca
      661 gcgaccgcac ccttggccgc atcgccaacc agcgtcaacg cggcggcgaa gctgctgccg
      721 gaccgcagca cgttggtcgc gccgggattg cgcgaaccgt acgtgcgggg atccgccagg
      781 cccatcagtc gactgaccag caccgcgaac ggcactgacc cgagcagata agccgcggcg
      841 atcgcggcca gattggcgag ccagacccac aggctttcca tcaggactcc cccagtgcgc
      901 agtcgaccgg ctgtgccccc agcgggtccg tcagcacgcg cggatccagt ccaaccagaa
      961 agccgcgacg tccgccgttg atgaagatgc gcggcagatc gagtatcgag cgctccacga
     1021 acaacggcag ggccttgcgc gtgttaaatg gggacgtacc gccgaccagg tagccggtgt
     1081 gtcgctgcgc gacatcgggc ctgcaggcct cgatgcgctt gcgcccgatc tggcgcgcca
     1141 ggttgcgggt cgaaacggtg cgatcaccgt gcatcagcac gatcagaggg ctgcccgcct
     1201 cgtcctccat caccagcgtt ttcaccacat gatgctcgag cacgccgagc gcgcgcgacg
     1261 aggccgcagt gccgccgtgc tcgatgtact catagacgtg ctcggtatat ggcactcgat
     1321 gctgcttcag ccactgcgtg gcgggcgtct cggacacatg cgcacgcttt gccatcgcgc
     1381 cagtctaggg cctgttcagc cccgcggatg gtgtcgcgcg tgcagcgcct gcaggcgctc
     1441 gcgcgcgaca tgcgtgtaga tctgggtcgt cgagatgtcc gcgtgaccca gcagcatctg
     1501 caccacgcgc agatcggcgc catggttcag caggtgcgtg gcgaacgcat ggcgcagtcc
     1561 gtgcggcgag agcggcgcgt gaatccccgc cgttcgcgca taccgcttga tcaggcccca
     1621 gaaccgctgc cgcgtcatcg cccgcgcgcg ccgggtgacg aaaagcgcgt cggcgccgcg
     1681 gcctgcgagc agcgcgggtc gagcctcgtc gagataccgc tgcagccacg cgcgcgcctc
     1741 ctcgccgagc ggaacgacac gctccttcga acccttgccg agcacgcgca ccagcccgtc
     1801 gttcagcgaa agctcaatca gcttcagacc cacgagttca gacacgcgca caccggtcgc
     1861 atacagcagc tcgagcatcg cgcgatcgcg caggccgagc ggcatttcgc gctgcggcgc
     1921 ggcgagcagc gcttcgacct gcgcctcgga aatcgcggcc gggaagcgct gcggccgctt
     1981 ggccggcgcc agttgcagcg tcggatcagc ttcgatcatc cgctcgcgca gcgcccagcg
     2041 ataaaagcga cgcatcgacg ccagccgccg gttggcggtc gaggaacgcg atgcggcaaa
     2101 tgtcgcggcg aagtatccgg tcagttgaac atcggtcgcg gtgacaagcg tcgcgccatc
     2161 gtggccggca agccaggcgg caagcccttc caggtcgcgc cggtacgctg cgagcgtatt
     2221 ggcggccagc ccgtcctcga gccaaaccgc atcgagaaac cggtcgaggt tgggatcgga
     2281 ccccgccccc gtcacgcccc gaccagcccc ttcttcagtc ctgcgtcggc cggcttgtcg
     2341 ccgatccaca cgcgcaacac cgcggtgaag aattcctcgc cggcgatcga attgcctttc
     2401 ggctcgccat tgacgacgat gcgggtgcca gcctccggtg tgaactcgaa gaagatcacg
     2461 ctgcccttct tcgcttcgcc gatcgacttc atgtttgact gcagcgcgtc gatctgcgcc
     2521 ttccatcccg cgagctgtgc ctcggtatgg ttagccttca gtccctccat cagcgcttcg
     2581 gcaaaggtgt cggcggatat gtcgcgcagc atgtggatct cgacccgccg tgggcccttc
     2641 tgcgccagca gcgcggctgc cgagttggcc ttctgcggca cgtacaggcc ggcggcgtag
     2701 accttgaaga acgcgcgcgt gcgcagcccg gcgccgttca gctgcagcgc ctgcccgccg
     2761 acttgcaccg tgggctcgaa cttcacgccc tcgatttcga ccggttgagc gcccacgccc
     2821 gatgcgaccg cggcgagcgc gagtgcggca atccatctct tcatctggtt tctccaattg
     2881 cgacggttaa gcggcctcgg gggtgacgct cttccccaac ccctttggca gcggaaacac
     2941 cacgttctcc tgtacgccct gcaacgtgcg cacgctgcca gcgccgagcg acttcaggcg
     3001 cgctgtcaac tgctgcacca attcctcggg cgcggaggcg cctgccgtga ctccgacccg
     3061 cgccgcgccc gacagccagt gcggctgcac gtcgcccgcc gagtcgacga gatacgcggg
     3121 cacgccgaag cgttccgcca cctcgcgcag gcggttcgag ttcgaactcg tcgggctgcc
     3181 gatcacgatc accagatcga cctgcggcgc catgaatttc accgcgtcct ggcggttctg
     3241 cgtggcgtag cagatgtcgg ccttcttcgg ctccgcgatc gccgggaaac gctgtttcag
     3301 cgccgacacg acgtcggccg cgtcatcgat cgacagcgtc gtctgcgtca cataggcgag
     3361 ccgatccggg tcgcgcaccg cgagccgctc gacatcggcg accgtctcca ccagatacat
     3421 gccgtcgtcg acctggccca tcgtgccctc gacctccggg tgaccggtgt ggccgatcat
     3481 cacgatctcg cgcccctgcg cgcgcatctt ctcgacctcg acgtgcacct tggtcacgag
     3541 cggacaggtt gcgtcgaaca cgcgcagtcc gcgcgcgctc gccgcagcgt gcaccgtctt
     3601 cgccacgccg tgcgcggaga agatcacggt cgcgccgtcg ggcacctggt cgagttcgtc
     3661 gacgaacacc gcgcccttgc cgcgcagcga ttcgaccacg aatgtgttgt gcacgatctc
     3721 gtgccgcacg tagatcggcg cgccgaagat ccgcagcgcg cgctcgacga tttcgatcgc
     3781 gcgatcgacg ccggcacaga atccgcgcgg ttgcgcgagc agcacttcca tcacagcact
     3841 ccgatgacct gcaccgagaa ccgcaccggc tggcccgcca gcggatgatt gaaatcgatc
     3901 acgacgcgat cctgatccac ctgcttcaac acgccggcca tgcgcccgcc attcggcgcg
     3961 tcgaactcga gcatctcgcc caccgcatgt gcggcacccg gatcggtgtg acgatcgaac
     4021 atcgcgcgcg tcaatgactg caccagctcc gggttgcgcg gcccgaaagc cagaccgggc
     4081 gcgagatcga acgtcgcgcg cgccccctct tccagtccga ccagccgctc ttcgagcgcc
     4141 ggcgccagtt gcccggcgcc gatctgcagg gtcgccggtc gactatccat cgtgctgacg
     4201 atttcgtgct cgcggtcgcc gacgcgaacc gccagtcgat aatgcagcgt gacatgtgcg
     4261 ccgggtgcga tgacagtcat gatcgttacg actagagcag gcagattcta tcgaaggagc
     4321 ccgcatgagc ctgaagaact ggccggcgtc ggaacgaccg cgcgagcggc tgctggcgca
     4381 aggtcccggc gcgctgagcg aagcagaact gctggccgtg ttcctgcgga ccggcgtgcg
     4441 cgggcaaacc gtgcttgact tggcgcggcg cgcactggat cacttcggcg gactgaacgg
     4501 cctattgtcg gcgccccaag gcgacctgca gcgaatcgcg ggattcggtc cggccaaatg
     4561 cgcgcaactg cacgccgtgc tcgaactggc gcgccgcgcg gtacgcgaag aggtccggcg
     4621 cgacacgttg ctgaacaccc cggcccgggt acgcgagtac ctgactctgt cgctgcgcaa
     4681 cgagcggcac gaggttttcg tcgcgctcta cctcgactcg cagaaccggc tgatcgtcga
     4741 cgaagtgctc ttccgcggca cgctcagcca gaccagcgtc ttcccgcgcg aggttgtgaa
     4801 acgggcgctg caccacaacg ccggcgcagt gatcttcgcg cacaaccatc cctccgggct
     4861 cgcggagccg agccgggccg acgaactcct gacgcgcgcc ctgaaagatg cgctcgcggt
     4921 cgtcgacgtc cgcgtactcg accacctgat cgtcgccggc cacgccagcg tttcgttcgc
     4981 cgagcgcggc ctgctgtgag cggccggcag tgcggttggc gcgcgagatc gaaagtcctt
     5041 ataatgcgcg gctttgcgcg acccggcccg ctgggcgcgg gtcgaactca ggagtccccg
     5101 atggcacgcg tgtgtcaagt caccggcaag aagcccatga cgggcaacaa cgtttcacat
     5161 gcgaacaaca ggaccaagcg ccggttcatg cccaacctgc agaatcgccg cttctgggtc
     5221 gaaagcgaaa agcgctgggt gcggctgcgc ctgaccaacg cgggactgcg cacgatcgac
     5281 aagctgggca tcgatgccgt gctcgcggat ctgcgtgcgc gcggtgagat ctgaggagga
     5341 cggtcatggc caagggcgca cgcgagaaga tcaagctgga atcgaccgcc ggtaccggtc
     5401 atttctacac cacgaccaag aacaagcgca ccactcccga gaagctggag atcagcaagt
     5461 tcgatccggt ggcgcgcaag cacgtgatgt acaaggaatc gaagctcaag tagcgccgcg
     5521 acgacggcga aaaaaagccc cgccgtgcgg ggcttttttg ttggcggcgg ccgcccgcgc
     5581 ttcagaccgg gatcgcgttc gtcgtgtagg agcggatcct gagcgccgca tcctgcagca
     5641 cgcggatgcc gctgacctcc gcgcgcgcga tccagtcctg aagatgtgcc agcatctgct
     5701 cgcgcgacac cgaggacctc tcccacgtga ccgcgaggtc ggcgcgcatc tcgatcaagg
     5761 tctgcagcac gcggctgcgg gtgagcaggt cacgcagcgt ctgctgctgt gtggccggga
     5821 tctgaccgac gtcgcccttg cgcagccagc gcgtaaggcg cgcgaagcgc gcgcgctcct
     5881 cggtcggcag gcgcagcttg tcgagttcgt cgcgcaatgt cttctgcagg ccgcgcgcgt
     5941 acttggccag caggtcgtag cggttcgaga tcaccgcctg cagcgtctgc acgtccaccg
     6001 gcgcgcgcgg cgccgcgacc cttgctacag gcgccacgcg caggatccgg gccagtccga
     6061 gcgcagacag gatgcggatg tacagccagc cgaggtcgaa ttcgtaccac ttgttcgaaa
     6121 gccgggcgga ggtcgggtac gtgtggtggt tgttgtgcag ttcctcgccg ccgatcagga
     6181 tgccccaggg tgagatgttg gaactcgcgt ccgggctatc gaagttgcga tagccccaga
     6241 agtgcccgat gccgttgatc acgccggcgg cgaacaacgg aatccagatc atctgcactg
     6301 cccacaccgc gataccggcg gcgccgaaaa gcagcacgtc ggcgaccagc atcaccgcac
     6361 agccctgcca ggtgaaacgc gaatagacgt tgcgctccag ccagtcgttt ggcgtgccgt
     6421 ggccgtagcg cgccagcgtt tccttgttct tcgcctccgt catgtagagc tcggcaccgg
     6481 tccacagcac cgtcttcagg ccgcgcgtct gcgggctgtg cggatcgtcc ggggtctcgc
     6541 acttcgcgtg atgtttgcga tggatcgcga cccactcctt ggtttgcatg ccggtggtga
     6601 accacagcca caggcggaag aagtgcgcaa cgatcgggtg caggtccaac ccacggtgcg
     6661 cttgcgagcg atgcaggaag atcgtcacgg ctgcgatcgt cacatgcgtc agagcgagcg
     6721 ccgcgacgat gagctgccac cacgtcaggt ccagcaagcc gccgtcgagg aattcaacca
     6781 gttgcatgaa gtctccgtaa agatggaagc agctgccgca gcctcccccg acgaccgcga
     6841 cgcgctcaag ggcgtaattg tacgtgcccg ggacagagcc gaacagggtt tcagcccggc
     6901 ccgcccaaac ccggattcca gcccgatatc cgcacttcgc gttgcgggaa ggggatcgcg
     6961 atacctgccg tgcggaagcg cgccaggatc tcctgggcga ccagcgattg cacgttcatc
     7021 cgcccctgtt ccggatcggc gatccagaat ccgacctcga ggtcgagccc atcggcgccg
     7081 aatccgagca gtaccgccga cggcgccgga tcgacaagca cgcgcgcggt cgagcgcgcc
     7141 gcctcctcca tcaaggcgag cgcccgctgc aggtcggagt cgtaagcgac cgcgaccttg
     7201 accgagaccc gcacgcggcg gtcggtgtac gagtggttgg tgaccggcga ggacaccagc
     7261 atctcgttcg gcacgatcgc ctcggtgccg tcgagcgcac gtacgaccgt atagcgcgtc
     7321 ctgatctggg ataccacgcc gtagtacttc tcgacggtga tcagatcgcc gatgcgcagg
     7381 ctgcggtcga gcaggatgat gaagccgctg acgtagttcg acgcgatccg ctgcaacccc
     7441 agtccgagtc cgacgccgag cgcgccgccg aacaccgaaa gcaccgtgag gtcgatcccg
     7501 gccagcgaca gcgcaagcag cacgccgacc gtgagcagca gcgcgcgcac cagtcgcgcg
     7561 acgaccacgc ggacgttcat gtcgaccgtc tcggcacgca gcagccgcgc ctcgagagcc
     7621 gaaccgatcc acagcgcaac gagcagcgta acgagcaccg agatcgtgcc ggtaaccagc
     7681 gtccacaggc tgaccggtgt gcgtcccagc ggcagcgtgt gcgcatcgag ccactgcacg
     7741 acgtcgtcca gcgcgccgac cagatgcagc gcgacaacca cccacaccac ggccgcgatc
     7801 gagcgctcga acacacccag gaacgccgcc cgcgtgaaca cccgtcgcag cacgtacacg
     7861 atgaggcgca ccagcgccat agcgccaagc agcgtcatcg ccagtctcag caggcgcacg
     7921 tcgccgggat gcggcagtcg ccccgtcacg cgcagcaggc cttcgcccgc ccacagcagc
     7981 aattgcgcgg cgatcggaaa cgccagccgc cggaagcctt cgatcgaaaa acgcagcgcg
     8041 tcggcctgcg tgccaccggc cgcgatccgc tgggccaatc gcgcttcgac gcgctgtcgc
     8101 acgatccggg atgcgaacca accaccgatg atcgcgcagc cgatcgcgat cgcgctccag
     8161 aagtgtgccg gtcgcgccag cccttcggcg agcgcctgca gcagttcctg cagcggcgct
     8221 tccgtcatcg ctttctctcc agcaccgcgg cgaagaagcc gtcggtgtcg tgcaggtgcg
     8281 gcagcatcac gaaccacggc gcgaagcgcg ccgcgtgatc gacttcgatc ttctgcgcgg
     8341 ccagcacatc ggccgccggc acgatgctga attcgccatg ctcggcaagg aagcgttcga
     8401 ccacctgctg gttctccagg cccagaagcg agcaggtcga atacaccagc cgcccgcccg
     8461 gtttgacgag acggctcgcc gcccgcagca aattgccctg cacctggttc acgcgcgcca
     8521 gttccgcctc gtcgaaacgc cacttcagat ccggattacg ccgcagcgtg ccgctgcccg
     8581 agcacggcgc atcgaccaac acgcggtcga tcttgccggc cagccgcttg acgcgcgcat
     8641 cgttctcgct cgcgatggcg gccgggtgca cgttggacag gccgcttcgc ttcagccgcg
     8701 ggccaaggcc ggccagccgc tttgcgtgaa tgtcgaacgc atagatgcgt ccgctcgatc
     8761 gcatcaggct gccgagcgcc agcgtcttgc caccggcgcc ggcgcagaaa tcgacgacca
     8821 tgtcgctgcg cttgggcgcg accagccgcg caatcagttg gctaccctcg tcctgcacct
     8881 cgatcagccc gtcctggtac agcggccacc gcgtcagagc cggcttctcc gccagccgta
     8941 tgccgtccgg cgcatagcgc gtcggatcgg ccgccagcgg cgcgtgctcg cgcgtggtct
     9001 tgtgcagttg ccccagcacg tcgtcgcgct gtgccttgat cgtgttcacg cgcagggtca
     9061 gcggcgccgg ttcggccagt gcggcgaaca gcggccctgc gtcgtggtac tgcgcggtca
     9121 cgtgagcgaa cagccacgtc ggcacttccg cgcgcatcgc gcccggcagg tgtgcgaggt
     9181 ccatcgccag cgcgtgacgc acagcgcgct cgtcgctcct cagcgcgcgc ggcccgagcg
     9241 cgtcgattcc atgctggcgc gccagcgcaa gcagcgccgc cagccggggg gcgcgcgcgg
     9301 gatgcgcggg cgccagttgc gcggtcagcg tcgcataccg gcgcagcacg aagaaagtcg
     9361 cctccgcgat caggctgcgg tcgcgcgccc cgagcgtgcg ctgctgcttg aagtagcgcg
     9421 acatcaccgc gtcggccggt ccgtcgaagc gcagcacctg acccagcagg tgcgcgagcc
     9481 cgtcgaccac ctgcgaggtg acgcgcactc cattcatggc gcgagcacgg actgcgcatc
     9541 ggcgtccaga tcgcgcgcgg caacgtgatc gccatcgagc cggatccggc cttcgcagac
     9601 ccagcgcacg cagcgcggca gcaaccgatg ttcctgcgcc agcacgcgcg cggcgagcgc
     9661 aacctcggtg tcgtcggcgc gcaccttgac tgccgcctgc gcgatgatcg gcccgccgtc
     9721 gacctcgttg gacacgaagt gcacggtgca gccgtgcacc ttgacgcccg cggcaagcgc
     9781 ctggcggtgc gtgtgcagcc ccggaaatac cggcagcagc gacggatgga tgttgatcaa
     9841 gcggccggca taacgatcga cgaagcgcgc cgtcagcacc cgcatgaagc cggccagcac
     9901 gaccagcacg ggatcgtgca cgtcgatcgc atcggcgagc gccgcctcga acgcgtcgcg
     9961 cgacgcatag tcgccgtgcg gaatgacacg cgtgcgaacc cccgcgttgc gcgcgaattc
    10021 gagccccgcg gcatcggcgc ggttgctgat cacgcacgcg atgcgcgccg cgaatgactg
    10081 ctcccagtct tcgtcgcgtg cactgcgcag gattgcgccg agattgctgc cgcgaccgga
    10141 gatcaggcac acgatgtttc gcatcgcggg cattttacgg tgcggcctgc aaacgcctcg
    10201 ctaaatacgc gtttcggcgg gtgcgctccg tataatcgca ctgcatcaag ccacgcgaac
    10261 gatgaagatt ttccgcggcc tccccccgcc ttcccaacgc catccgtgcg cgctggcgat
    10321 cggcaatttc gacggtgtgc atcgcggtca ccgcgcgctg ctcacgcgcg tggtccaggc
    10381 cgcgcggcag cgcgggctga tctcggcagt gatgacattt gaaccgcatc cgcgcgaact
    10441 cttcgcaccg gacaaggcgc cggcacgcgt ggctaatctg cgcgacaagc tcgaagggct
    10501 gaagacctgc ggcatcgacc acgtgtatct gcagcacttc aaccggcgct tcgcgtcggt
    10561 cggcgccgcg gacttcatcg accgcatgct ggtcgaaagg tgccggatga agtggctgct
    10621 ggtcggcgcg gacttccgct tcggcgcgca ccgcgccggt gaccttgcgc tgctgcgcgc
    10681 gcatgcggcg cgtggcggct acgaagtcga gcaactggaa accgtcacca acgacggcga
    10741 gcgcgtttcg agttccgcgg tgcgcgcggc attggcgcgg ggcgatctcg cgcgcgccgc
    10801 gcatctgctc ggccatcctt atgcaatcac cgggcgcgtg ctgcacggcg cgaagcgcgg
    10861 gcgcacgctc ggctatccga cgctgaacct gcgcatcgcg cacaagcgtc cggccgcgca
    10921 aggcatctac gcggtgtgcg tgcacggcat cggcgatgcg ccttggccag gcgtggcgag
    10981 cgtgggcgtg cggccgaccg tggaggacag cgggcgctgg ctgctcgaag tgcacctgtt
    11041 cgatttcgcc gaccagatct acggccgcct catcagagtc gagttcataa agaagctgcg
    11101 cgacgagacg aagtacgaca cgctcgatgc gttgaccacc gcgatccgca acgacgccga
    11161 tcaggcacgc gcgctgctcc tgagcagcgg aatttgattt ccgatccacc ttgaagagcc
    11221 cgagggggcc aaggaaccat catggctgac gacgccaaac gccctgcgtc caagatgaag
    11281 ccgcccgccg atccggcgag caagaaatac ccgctgaatc tgcccgacac gccgtttccg
    11341 atgcgcggcg atctgcccaa gcgcgagccg cagtgggtgc aggaatggga tgcccgcaag
    11401 gtgtaccaag cgatccgctc ggcgcgcaaa ggcgcgccga agttcatcct gcacgacggg
    11461 ccgccgtatg caaacagcga gattcatctc ggcacggcgg tcaacaagat cctgaaggat
    11521 atcgtcgtaa agacgcgctc gatggccggt ttcgacgccg tgtacgtgcc gggctgggac
    11581 tgtcacggca tgccgatcga gatccacatc gagaagaaat tcggcaagag cctgccgcgc
    11641 gccgaggtgc aggcgaaagc gcgcgcgcac gcgaccgagc agatcgcact gcaaatgcag
    11701 ggcttcaaac ggctcggcgt gctcggcgat tgggacaacc cctacaagac gatgacgttc
    11761 aaggccgaag cggacgagat ccgcacgctc ggccgcatga tcgagcgggg cttcgtgtac
    11821 cgggggctga agccggtgaa ctggtgcttc gactgcaact cggcgctggc cgaggccgag
    11881 gtcgaataca tcgacaagaa gacgccggtg atcgacgtcg cctttccgct cgccaaggcc
    11941 gacgagccaa aggtcgccgc tgcgttcggc gtggccgcgc tcggcaagcc gaccgcgacc
    12001 gtgatctgga ccacgacacc gtggacgatc ccggccaacc aggcgctgaa tttccatccg
    12061 gacttcatct acgcgctggt cgactgcggc gaccggctgc tcatcctcgc ggaatcgctg
    12121 gtcgagtcgt gcctgcagcg ctacggactg aaggggcgtg tgctggcaac ctgcatcggc
    12181 aaggcgctgg aactggtgcg cttccgccat ccgctggcgc cgctcgacaa ggggtacgac
    12241 cgcgattcgc cggtatacct cggcacctac gtgactgcgg atgcgggcac cgggatcgtg
    12301 cactcctcgc ccgcatacgg cgtggaggac ttcgattcgt gcaagtcgta cgggatgacc
    12361 aacgaccaga ttctcaatcc ggtgaccggc gatggtacct atgcgaagga cttcccgctg
    12421 ttcggcggcc tgcggatctg ggacgcgaac ccgaaagtgg tcgacgcgct gcgcgtggct
    12481 ggcgcgctgc tgcacgcgtc ggccgacacg cacagctaca tgcactgctg gcggcacaag
    12541 acgccgctga tctaccgcgc cgcctcgcag tggttcgtgc gcatggatgg tgccacgcgc
    12601 gaaacgcagg gcgtgatgca gcgggagacg gtcggcgaaa cgctgcggca gactgcgctg
    12661 cgggcaatcg agcagaccgc gttttatccg gcatggggca aggcgcggct gcacggcatg
    12721 atcgccaatc gacctgattg gtgcatctcg cgccagcgca actggggcgt gccgctgccg
    12781 ttcttcctgc accgggaaac gggcgaactg cacccgcgca cgctcgaact gctggaggtg
    12841 gcagcgcaga aggtcgaaca gggcggtgtc gaggcctgga gccgcgcgac cgtcgaggat
    12901 ttcctgtcgc cggccgaggc catgcagtac acgaagatca acgacatcct cgacgtgtgg
    12961 ttcgactcgg gctcgaccca tcgcacggtg atgcgcggct cgcacgcgtc cgaactcgcc
    13021 tacccggccg acatgtacct cgagggctcg gaccagcatc gcggctggtt ccactcgtcg
    13081 ctgctgaccg gcgcggcgat cgacgggcgc gcgccgtaca aggcgctgct gacccacggc
    13141 ttcgtggtcg atgcgctcgg gcgcaagatg agcaagtcgc tcggcaatgg catcgacccg
    13201 cgcgaactcg ccgaccagat gggtgcggag atcatgcgtc tgtgggtcgc gtcgaccgac
    13261 tattcgggcg agatgtcgat cggcgatgaa atcctgaagc gcgttgtcga gagctatcgc
    13321 cgcatccgca atacgttacg gttcctgctc gccaatacca gcgacttcga cgttgccagg
    13381 gacgcggtgc cgatcgggga actggtggag atcgaccgct acgcgatcgc actgaccgcg
    13441 caaatgcagg ccgaggcgca ggcaaactgt gaccgcttcg aattccaccc ggtgattgcg
    13501 cggctgcaga tcttctgctc cgaggacctc ggtgcgttct atctggacat cctgaaggac
    13561 cggctgtaca cgacggcgcc ggattcgcgt gcgcggcgct cggcgcagac ggcgctctgg
    13621 cacatcacgg cgagcctgct gcggctgatg gcgccgtatc tgtcgttcac ggccgaggaa
    13681 gcgtactggg tgttcgtccg cgacgcggac gcgacgatct tcaccgacgt ctaccacgag
    13741 ttgccgcccg tgccggggca cgaggcgttg ctctccaagt gggcgcagat ccgtgccgcc
    13801 cgtgccgagg tgctcaagca gatcgaagcg ctgcgcgagg ccggcaagat cggctcgtcg
    13861 ctgcaggccg aggccgacct gcacgcgtcc ggtgagcgcc atgccttgct tgccaccctg
    13921 gacgatgatc tgaagttcgt gctgatcacg agccgcgcga cgctgcatgc ggcggcgaag
    13981 ccggccgacg agaaaatcat cgtcacgccg acggctcaac gcaaatgcga ccgctgctgg
    14041 cactggcgcg ccgatgtcgg cggccacgcc gagcatccca cgctgtgcgg gcgctgcgtg
    14101 accaatctct tcggcgccgg tgagccgcgc agggcggcct gatggcctgg agcggcggca
    14161 gcacgtggcg gcagcggctg gtgccttggc tggggctttc gaccgtgatc gtgctggccg
    14221 accagctgac caagatcgtc atcgaacgca agttcgagtt cggtgacgtg catccggtca
    14281 cgggcttctt caacctggtg ctgacctaca acaagggcgc ggcgttctcc tttctcgcca
    14341 atgcgtcggg ctggcagagc cggttcttca ccgcggtcgg gatcgcggcg tccctgttca
    14401 tcgtctacct gctggcgcgg cacggcgccc agcgcttgtt cgccctgtcg ctggcgctga
    14461 tcctcggcgg cgcggtcggc aacgtgatcg atcgcctcgt gcacggccac gtgatcgact
    14521 ttctcgattt ccactggcgc ggctggcact ggccggcatt caatctcgcc gactcggcga
    14581 tcgtcggcgg cgccgcattg ctgatcctcg acgagctatt gcgtgtgcgg aaatccaagt
    14641 aatctcaccg ccatggatct ggacggcaag cgcatcgtgc tcggcatgac gggcggcatc
    14701 gccgcctaca aggtgtgcga gctcgcgcgg cggctgcagg atgacggcgc cgacgtgcag
    14761 gtcgtgatga cacgcggggc ggtgcagttc gtcaccgcga
//
LOCUS       DBNHBY010000668         5222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_657751, whole genome shotgun sequence.
ACCESSION   DBNHBY010000668 DBNHBY010000000
VERSION     DBNHBY010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_657751"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..910)
                     /locus_tag="AC16HB_15915"
     CDS             complement(107..910)
                     /locus_tag="AC16HB_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608326.1"
                     /translation="MPSALDHIVIVSNDLDTAIANATKAGFTVVPGGTHGSGNTHNAL
                     IGFADGAYIELFAPTDRGRTGEHRWFERLRNGGGLVDFCLLATDLDAEIDAIRARGIE
                     YSRPFAMERVTPSGVRLAWRLSTAPGAVGATGWPFMIEDTTPRMLRVPHAPAQIGHAN
                     GVIGVAGITVLVRDTEASARNYEAILGARAQALAAPSGDRRPGAIVPVGAAWILLAEP
                     ATEEEREHLERHGQGPYRITLRRHRESVSPGAGSLLDARLFSGAKIAIA"
     gene            complement(926..1906)
                     /locus_tag="AC16HB_15920"
     CDS             complement(926..1906)
                     /locus_tag="AC16HB_15920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5608327.1"
                     /translation="MTLATRIRALFAAALACTTGGTVAQTWPDHPIKLIVPFPPGGGT
                     DGVARSVATHLSAALGQPVVVDNKAGAGGTIGANAVATATPDGYTIGIATSSTHPAAM
                     VLQKNVPYHALKSFEPITMIASTSYVLIGSPALPAANLAELIAHAKAMPGKLNFANVG
                     TSTLGYLLSRQMKALSETDMVDVSYKGSSQVYPDLMSNQVSLFLDNPGASTPLVAAGR
                     LKAFAVTTPTPSMPGVPLFKDAGRNVGLQDFDSAFWYGLVAPAGTPRAIVERIQAETA
                     KFVNSAEGKKEFATRSLDPVGSTSVQFGATIEKDIERFTALAKQFNIQAQ"
     gene            complement(1910..2785)
                     /locus_tag="AC16HB_15925"
     CDS             complement(1910..2785)
                     /locus_tag="AC16HB_15925"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5608328.1"
                     /translation="MQLARIRGSDGRPVAAAWVEGRVVELPIAQHAAFADLATLLSAG
                     DAALDRAQSYAREAIAAGKGIDAGRVTFLAPTTPAVFLGLGYNYRALATNEGLPFNKH
                     PELFAKMPGCAIGHDQPVRVPKVIDKVDFEAELAVVIGRTARRIKAKDALGYVGGYTA
                     CNDVTAKVIPRPPESGSIVVPLKAADTFGPMGPTLVPARGIDPQDLTLLCRVNGIERQ
                     RFSTRDMVHSVAEVIEYVTARITLNPGDVITTGTSLGIGIIQKPPVFLREGDVVEVQI
                     EGFPALRNRIEWEKE"
     gene            3005..3418
                     /locus_tag="AC16HB_15930"
     CDS             3005..3418
                     /locus_tag="AC16HB_15930"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5608329.1"
                     /translation="MEITGGCLCRSVRYRITAAPIVTRVCWCRVCQFLGAGSGTVNTC
                     FPRDAVSIEGELHDYRSVADSGNVMHRRFCPRCGTPMFSEAESRPHLIFVRSGTLDDV
                     ELAKPALTIWTSRAPTWACIDAALPRTEGQPPPAA"
     gene            complement(3461..4543)
                     /gene="apbC"
                     /locus_tag="AC16HB_15935"
     CDS             complement(3461..4543)
                     /gene="apbC"
                     /locus_tag="AC16HB_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR5608330.1"
                     /translation="MLTVDRVNEVLKTIIDPNTGKDLLATKSARNVRVEGGAVSLDVE
                     LGYPAASQIEPLRARVVEALRTAGAASVNANVTMKIVAHTVQRGLKVMPNVKNIIAVA
                     SGKGGVGKSTVAVNLALALTAEGARVGMLDADIYGPSQPTMLGVSGQPESKDGNTMEP
                     LENYGIQVSSIGFLVDADQPMIWRGPMVTQALQQLLMQTNWKNVDYLIVDMPPGTGDI
                     QLTLAQQVPVTGAVIVTTPQDIALIDAKKGLKMFEKVGVPILGIVENMAIHVCSQCGH
                     AEHIFGEGGGAKMASQYGVDVLGSLPLDIRIREQADSGKPTVVAAPDSDLTEAYKAIA
                     RKVAVKIAEKSKDMSLKIPTIKVQHT"
     gene            4685..>5222
                     /locus_tag="AC16HB_15940"
     CDS             4685..>5222
                     /locus_tag="AC16HB_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5608331.1"
                     /translation="MQPRQIFVTTALPYANGKPHIGHIMEYIQADIWVRYQRLRGHAV
                     HFVCAEDAHGAAIMIAAEKAGRTPQQFIAEMMAARQQYLDGFHIGFDHWHTTDSKENV
                     ELSQSIYRGLRANELIDIRTIEQFYDPLKGMFLPDRYIKGTCPKCGAKDQYGDNCEVC
                     GATYLPTDLKEPYSVLTGA"
BASE COUNT          791 a         1749 c         1760 g          922 t
ORIGIN      
        1 gcgctcatca ctgccgacgg attcgtgcgc gagatctcga tcgccgtgcc ctgattccgt
       61 cgcacgacgc gtgcgcgcgg acgagagcgc ggcggctttt cgtcgctcag gcgatcgcga
      121 tcttcgcacc cgagaagagc ctggcatcga gcaaagagcc ggcgcctggg ctgacggatt
      181 cgcgatggcg gcgcagcgtg atccggtacg gcccctggcc atgacgctcg aggtgctctc
      241 tttcctcctc ggtcgcgggc tcggcgagca ggatccacgc ggcgccgacc ggaacgatcg
      301 cgcccggcct gcggtcaccc gacggcgctg ccagcgcctg ggcgcgcgct ccgaggatcg
      361 cctcgtagtt ccgcgcggat gcctcggtgt cgcgaaccag cacggtgata ccggcgaccc
      421 cgatgactcc gttcgcatgg ccgatctgtg ctggcgcatg cggcacacgc agcatgcggg
      481 gggtcgtgtc ctcgatcatg aatggccatc ccgtcgcgcc caccgcgccc ggtgccgtgc
      541 tcagccgcca tgccagccgg acaccgctcg gagtcacccg ctccatcgcg aacggccgcg
      601 aatattcgat cccgcgcgca cggatcgcgt cgatctcggc gtccagatcc gtggcgagca
      661 gacagaagtc cacgaggccg ccgccattgc gcagccgctc gaaccatcga tgctctcccg
      721 tgcgtcctcg atccgtgggc gcgaagagtt cgatatacgc gccgtcggcg aacccgatca
      781 gcgcgttgtg ggtgttgccg ctgccgtgcg ttcctcccgg cacgacggtg aagccggcct
      841 ttgtcgcatt cgcgatcgcg gtgtcgaggt cgttcgagac gatgacgatg tgatcgagcg
      901 cggacggcat cggcggtcaa ccgcgtcact gcgcctggat gttgaactgt ttggccagcg
      961 cggtgaagcg ctcgatgtcc ttctcgatgg tcgcgccgaa ctgaacggag gtgctgccca
     1021 cgggatccag gctgcgcgtc gcaaactcct tcttgccctc ggcgctgttc acgaactttg
     1081 ccgtctcggc ctggatgcgc tccacgatgg cgcgcggtgt gcccgccggc gcgacgagcc
     1141 cataccagaa ggcggagtcg aagtcctgca ggcccacgtt cctgcccgcg tccttgaaca
     1201 gcggcacgcc cggcatcgaa ggcgtcggcg tcgtcaccgc gaacgccttc aggcgtccgg
     1261 cggcgacgag cggtgtcgaa gcgccggggt tgtcgaggaa gagcgagacc tggttcgaca
     1321 tcaggtccgg atagacctgc gacgagccct tgtacgaaac gtcgaccatg tcggtctcgc
     1381 tgagcgcctt catctgccgg ctcaggaggt agcccagcgt cgaggtgccg acgttggcga
     1441 aattgagctt tccgggcatc gccttcgcat gcgcgatcag ttccgcgagg ttcgcagcgg
     1501 ggagtgccgg actgccgatc agcacgtagg acgtggaggc aatcatcgtg atcggctcga
     1561 agctcttcag cgcgtgatag ggaacgttct tctgcaggac cattgcggcg gggtgcgtgc
     1621 tggaagtggc gatgccgatc gtgtagccgt ccggcgtcgc ggtggcgacc gcgttggcgc
     1681 cgatggtgcc gccggcaccg gccttgttat ccacgaccac cggctgcccg agcgcggccg
     1741 aaagatgcgt cgcgaccgaa cgtgcgacgc catcggtgcc gccccccggc gggaatggca
     1801 cgatcagctt gatgggatgg tcgggccacg tctgggccac cgtgccgccc gtggtgcatg
     1861 ccagagcggc ggcgaagagc gcgcggattc gagtcgcaag cgtcatggcc tactcctttt
     1921 cccattcgat ccggtttcgc agcgccggga atccctcgat ctgcacttcc accacgtcgc
     1981 cttccctcag gaagaccggc ggcttctgga tgatcccgat cccgagcgag gttcccgtgg
     2041 tgatgacatc gcccgggttg agcgtgatgc gcgccgtcac gtattcgatc acctcggcca
     2101 cgctgtgcac catgtcgcgg gtcgagaagc gctgccgttc gatgccgttc acacggcaca
     2161 gcagggtgag atcctgcgga tcgatgccgc gcgcgggaac gagcgtcgga cccatggggc
     2221 cgaacgtgtc tgccgccttc agcggcacga cgatgctgcc ggactcgggc gggcgcggaa
     2281 tcactttcgc ggtgacgtca ttgcaggccg tgtagccgcc gacgtagccg agcgcgtcct
     2341 tcgccttgat gcggcgtgca gtgcgtccga tcacgaccgc cagttcggcc tcgaagtcga
     2401 ccttgtcgat gaccttcggc acgcgcacgg gttggtcgtg cccgatggcg caccccggca
     2461 tcttggcgaa gagctccggg tgcttgttga agggcaggcc ttcgttggtc gccagcgccc
     2521 tgtagttgta gcccaggccg agaaagaccg cgggcgtggt gggcgcgagg aacgtgacgc
     2581 ggccggcatc gattcccttg cccgccgcga tcgcctcgcg cgcatacgac tgcgcgcggt
     2641 cgagggcggc gtctcccgcg gacagcagcg tcgcgagatc cgcgaaggcc gcgtgctgtg
     2701 cgatcggcaa ctcgaccacc cggccctcga cccatgccgc ggcgaccggt ctgccgtcgc
     2761 ttcccctgat tcgagccagc tgcatacctg cgcctccgcg gttcccatcc agaatgaacg
     2821 cgcattttcc gcctgtccag attcaggttc acgggagtgg cgcgaggcgc cttcgccggg
     2881 gagcgaagat caggcggcga cttgcgatcc gcgatggtcg atcggttaat ctgcgcggat
     2941 ccgacgcagt gctgcagcgg cgctcgagcg caggggcgtg ggtgaactac cgcgaggtca
     3001 gacgatggaa atcacgggcg gctgtttgtg cagatcggtg cggtatcgaa tcacggctgc
     3061 acccatcgtg acgcgggtct gctggtgccg cgtctgtcag tttctcgggg ccggcagcgg
     3121 gaccgtgaac acctgtttcc cgagggacgc cgtttccatc gagggcgagc tgcacgacta
     3181 tcgcagcgtc gccgacagcg gcaacgtgat gcatcgccgc ttctgcccgc ggtgcggcac
     3241 gccgatgttc agcgaggccg agtcacggcc gcacctgatt ttcgtgcgca gcggcacact
     3301 ggatgacgtg gagctggcaa agccggcgct gacgatctgg acgtcccggg cgccgacctg
     3361 ggcatgtatc gacgccgcgc ttccgcggac tgaagggcag ccgccgccgg ccgcttagcg
     3421 tgcaccggtt cgttcgcggg caacggccgc tccgcgcgcg ctacgtgtgc tgcaccttga
     3481 tggtcgggat cttcagcgac atgtccttcg acttctcggc gatcttcacc gcgaccttgc
     3541 gcgcgatcgc cttgtaggcc tccgtgaggt cgctgtcggg cgcggcgacg acggtcggct
     3601 tgccggagtc cgcctgctcg cggatccgga tgtcgagcgg cagcgacccg agcacgtcga
     3661 cgccgtactg gctcgccatc ttcgcgcccc cgccttcgcc gaagatgtgc tcggcgtggc
     3721 cgcattggct gcacacgtgg atcgccatgt tctcgacgat gccgaggatc ggcacgccga
     3781 ccttctcgaa catcttcagc cccttcttgg cgtcgatcag cgcgatgtcc tgcggcgtgg
     3841 tgacgatcac cgcgccggtg accgggacct gctgcgcgag cgtgagctgg atgtcgccgg
     3901 tgcccggcgg catgtcgacg atcaggtagt cgacattctt ccagttggtc tgcatcagca
     3961 gttgctgaag cgcctgcgtg accatcgggc cgcgccagat catcggctgg tcggcgtcga
     4021 ccaggaagcc gatcgagctg acctggatgc cgtagttctc cagcggctcc atcgtgttgc
     4081 cgtccttcga ttccggctgg ccgctgacgc ccagcatggt cggctggctc ggcccgtaga
     4141 tgtcggcgtc gagcatgccg acgcgcgcgc cctcggcggt gagcgccagc gcgaggttga
     4201 ccgccaccgt gctcttgccg acgccgccct tgccggacgc gaccgcgatg atgttcttga
     4261 cgttcggcat caccttcagc ccgcgctgca cggtgtgcgc gacgatcttc atcgtcacgt
     4321 tcgcgttcac gctcgcggcg ccggccgtcc gcagcgcttc gaccacgcgc gcgcgcagcg
     4381 gctcgatctg gctcgcggcc ggatagccga gttcgacgtc gaggctgacc gcgccgccct
     4441 cgacgcgcac gttgcgcgcg gatttggtcg ccagcaggtc cttgccggtg ttgggatcga
     4501 tgatcgtctt gaggacttcg ttgacgcggt cgacggtgag catgtctgtc ggattcagga
     4561 gtcgggatcg aggatgcagc gggagtctac ggcaccggcc cgcagcgatg cccttgctaa
     4621 gatgccggcc attccgcgct cggtgatgca ccgcccgacg cgctccgcca tccgcgatcc
     4681 gagcatgcag ccacgccaga tcttcgtcac caccgcgctg ccgtacgcga acggcaagcc
     4741 gcacatcggc cacatcatgg agtacatcca ggccgatatc tgggtgcgtt accagcggct
     4801 gcgcgggcac gcggtgcact tcgtctgcgc ggaggacgcg cacggtgcgg cgatcatgat
     4861 cgccgccgag aaggcgggca ggacgccgca gcagttcatc gcggagatga tggccgcgcg
     4921 ccagcagtac ctcgacggct tccacatcgg tttcgaccac tggcacacga cggactcgaa
     4981 ggagaacgtc gagctgtcgc agtcgatcta ccgcggactg cgcgccaacg agctgatcga
     5041 catccgcacc atcgagcagt tctacgatcc gctcaagggc atgttcctgc ccgaccgcta
     5101 catcaagggc acctgcccca agtgcggcgc caaggaccag tacggcgaca actgcgaagt
     5161 gtgcggcgcg acgtacctgc cgacggatct caaggagccg tactcggtgc tgaccggcgc
     5221 ca
//
LOCUS       DBNHBY010000669         2702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_319899, whole genome shotgun sequence.
ACCESSION   DBNHBY010000669 DBNHBY010000000
VERSION     DBNHBY010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_319899"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC16HB_15945"
     CDS             complement(<1..474)
                     /locus_tag="AC16HB_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HIR5608332.1"
                     /translation="MEHYADLLVKSIFLENMALAFFLGMCSFLACSKKVETALGLGVA
                     VVFVQVLTVPLNQVILKQLLGEGALAWLHPALASVDLTFLAFILFIGTIAAAVQVVEM
                     AVDRFAPKRYTTLGVFLPLIAVNCVILAGSLFMQERDYTLAESVVYGVGSGLGWAL"
     gene            complement(486..1133)
                     /locus_tag="AC16HB_15950"
     CDS             complement(486..1133)
                     /locus_tag="AC16HB_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HIR5608333.1"
                     /translation="MSAVSETLFGKPQRNALIGPIVSNNPIGVQVLGICSALAVTTRV
                     DKAVVMGIGVTLVTAFANLAVSLVRNHTPSSIRIIVQLAIIASLVIVVDQFLKAYLFE
                     LSLELSVFVGLIITNCIVMGRAEGYAMQNPPWLSLLDGVGNGLGYALVLLLVAVAREL
                     LGAGKILGISVLRAQSDGGWYEPNGLMLIAPSGLFLLALLVWGLRTLKPELQEKD"
     gene            complement(1130..1924)
                     /locus_tag="AC16HB_15955"
     CDS             complement(1130..1924)
                     /locus_tag="AC16HB_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HIR5608334.1"
                     /translation="MARTDSTRYTFFFAAAVCIACALLVSVAAVGLKDKQETNARLYR
                     QKNVLLAAGLVKPGDDVSDRELLQIFDRNIRIRLVDLKSGALLPAGKIDEKNYDQRRA
                     RNDPQASRAAPPNAAQIQMLPNYAAVYLVQAQSGAVEQVVLPVEGVGMWGTCYGFVAL
                     DRDGNTVRGLTFYEQKETPGLGGEISNPKWQALWRGRKVFDAQWQPKLTVIKGHGGPP
                     EQDPHRVDALSGATITSNGIARLMAFWFSDDGFGPYLKRFREGAAS"
     gene            complement(1911..>2702)
                     /gene="nqrB"
                     /locus_tag="AC16HB_15960"
     CDS             complement(1911..>2702)
                     /gene="nqrB"
                     /locus_tag="AC16HB_15960"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HIR5608335.1"
                     /translation="EVNEGFFVTGILFPLILPPTTPLWQAALAISFGVVLAKEVFGGT
                     GMNFINPALAARAFLFFAYPAEISGDRVWTAVPPNVAVDGFSGATMLAQARQMTGAFD
                     SLGLSWWNAFIGLEPGSMGETSALACLLGAVLLIATGIGSWRTMAGVTLGTIAMALAF
                     NALASPAHPYFAIPFWWHMVLGGWAFGTVFMATDPVTSPFTERGKWIYGILIGALVVL
                     VRTVNPAYPESMMLVILFMNVLAPLIDYPIVKANINRRAKRHGAH"
BASE COUNT          506 a          928 c          856 g          412 t
ORIGIN      
        1 gagcgcccag ccgagccccg agccgacgcc gtagaccacg ctttcggcca gcgtgtaatc
       61 gcgctcctgc atgaagagcg aaccggccag gatcacgcag ttcaccgcga tcagcggcag
      121 gaacacgccg agcgtcgtgt accgcttcgg cgcgaagcgg tcgaccgcca tctcgaccac
      181 ttgcaccgcg gcggcaatcg tgccgatgaa caggatgaag gcgaggaacg tcaggtcgac
      241 gctcgccagc gccggatgca gccacgccag cgcgccctcg ccgagcagct gcttcaggat
      301 gacctggttc agcggcacgg tgagcacctg cacgaatacc accgcgacgc cgagtccgag
      361 cgcggtctcg accttcttcg agcaggcgag gaacgagcac atgccgagaa agaaggcgag
      421 cgccatgttc tcgaggaaga tcgatttcac cagcaggtcg gcgtagtgct ccatgttgtg
      481 agctcctaat ccttctcctg cagctcgggc ttgagcgtcc gcagccccca caccagcagc
      541 gcgagcagga acaggcccga cggcgcgatc agcatcagcc cgttcggttc gtaccagccg
      601 ccgtcggact gcgcgcgcag cacgctgatg ccgaggatct tgccggcgcc cagcagctcg
      661 cgcgcgaccg ccaccagcag cagcaccagc gcatagccga gcccgttgcc gacgccgtcg
      721 agcagcgaca gccacggcgg gttctgcatc gcgtaaccct cggcgcggcc catcacgatg
      781 cagttggtga tgatcagccc gacgaacacc gagagttcca gcgacagctc gaacagatac
      841 gccttcagga actggtcgac cacgatcaca agcgaggcga tgatcgccag ctgcacgatg
      901 atgcggatcg aactcggcgt gtggttgcgc accaggctca ccgccagatt ggcgaacgcg
      961 gtcaccagcg tcacgccgat ccccatcacc accgccttgt cgacccgcgt ggtcaccgcg
     1021 agtgccgagc agatgccgag cacctgcaca ccgatcgggt tgttgctgac gatcggaccg
     1081 atcagcgcgt tgcgctgcgg cttgccgaac agtgtctccg agaccgcgct cacgaggcag
     1141 ctccttcacg aaagcgtttc agatacggac cgaagccgtc gtcggaaaac cagaacgcca
     1201 tcagtcgcgc gatgccgttg ctggtgatcg tcgcgcccga cagcgcgtcg acgcggtgcg
     1261 ggtcctgctc gggcggacca ccatggccct tgatcaccgt gagcttcggc tgccattgcg
     1321 catcgaacac cttgcgaccc cgccacaacg cctgccactt cggattgctg atctcgccgc
     1381 cgagtcccgg cgtttccttc tgctcgtaga acgtcagccc gcgcacggtg ttgccgtcgc
     1441 ggtcgagcgc gacgaagccg tagcaggtgc cccacatgcc gacgccctcg accggcagca
     1501 cgacctgttc gaccgcgcca ctctgcgcct gcaccaggta cacggccgca tagttcggca
     1561 acatctggat ctgcgccgcg ttaggcggcg cggcgcggct cgcctgcggg tcgttgcgcg
     1621 cacggcgctg gtcgtagttc ttctcgtcga tcttccctgc gggcagcagt gcgccggatt
     1681 tcagatcgac caggcggatg cggatgttcc ggtcgaagat ctgcagcagt tcgcggtcgg
     1741 acacgtcgtc gccgggcttg accagccccg ccgcgagcaa cacgttcttc tgtcggtaca
     1801 gccgcgcgtt ggtctcctgc ttgtccttga gcccgaccgc ggcgaccgac accagcagcg
     1861 cgcacgcgat gcataccgct gcggcgaaga agaacgtgta gcgggtcgaa tcagtgcgcg
     1921 ccatggcgct tcgccctgcg gttgatgttg gccttgacga tcggatagtc gatcagcggc
     1981 gccagcacgt tcatgaacag gatcaccagc atcatcgatt ccgggtacgc cgggttcacc
     2041 gtgcgtacca gcaccacgag cgcgccgatt aagatgccgt agatccactt gccgcgttcg
     2101 gtgaacggag aagtgaccgg atcggtcgcc atgaacacgg tcccgaacgc ccagccaccg
     2161 agcaccatgt gccaccagaa aggaatcgcg aagtacggat gcgcgggcga cgcgagcgcg
     2221 ttgaacgcaa gcgccattgc gatcgttccg agggtgacgc ccgccatcgt ccgccaggag
     2281 ccgatgccgg tcgcgatcag cagcacggcg ccgagcaggc acgccaacgc cgaggtctcg
     2341 cccatcgagc cgggctccag cccgatgaac gcgttccacc acgagagccc gagggaatcg
     2401 aacgcgcccg tcatctgccg ggcctgggcc agcatcgttg cgccggagaa gccgtcgacg
     2461 gcgacgttcg gcggcacggc ggtccaaacg cggtcgccgg agatctcggc cggataggcg
     2521 aagaacagga acgcgcgcgc cgccagcgcc gggttgatga agttcatccc ggtgccgccg
     2581 aagacctcct ttgccagcac gacgccgaag ctgatcgcga gcgccgcctg ccacagcggc
     2641 gtggtcggcg gcagaatcag tggaaacagg atgccggtga cgaagaagcc ctcgttgact
     2701 tc
//
LOCUS       DBNHBY010000670         1881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1063349, whole genome shotgun sequence.
ACCESSION   DBNHBY010000670 DBNHBY010000000
VERSION     DBNHBY010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1063349"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16HB_15965"
     CDS             <1..447
                     /locus_tag="AC16HB_15965"
                     /inference="COORDINATES: protein motif:HMM:NF042682.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HIR5608336.1"
                     /translation="STQFEHWDRLAFHMAPPLLVRPGPDGRPRKIEFGPWLHGALRML
                     AKLRWMRGTALDVFGRSEERRLERQLRDDYEAMIDGLLSALRPDNHALAQQIASVPEQ
                     IRGFGHVKLANITLARARWKDLLDRFHGRAPAAQPVVVQLQRERAG"
     gene            complement(444..794)
                     /locus_tag="AC16HB_15970"
     CDS             complement(444..794)
                     /locus_tag="AC16HB_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5608337.1"
                     /translation="MTIKRLHVGPRMSEAVICNGMVFLAGQVADDAKQDAAGQTKQVL
                     AAIDQLLADAGTDKSRLLSASIFLADMADFAAMNSVWDAWVSPGNTPARATVEATLAR
                     PGWLVEIVVTAALP"
     gene            complement(799..1671)
                     /locus_tag="AC16HB_15975"
     CDS             complement(799..1671)
                     /locus_tag="AC16HB_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5608338.1"
                     /translation="MPFESPRFSSVKCLSPAGFHRMAYTEWGDPGNANVLLCVHGLTR
                     VGRDFDRLARAMFDRYRVVCPDVAGRGASDWLRDPRHYHVGQYASDMVTLIARLNVES
                     VHWIGTSMGGLIGITVAGQPASPVTKFVINDVGPTLSAPAIQRIGEYLGKPIRFDSID
                     AATDYIALVGAPFALRTREEWREITETVIRRDGDGWVLHYDPRIAEPFKSVTPEIAAQ
                     AEAATWALWDNIRCPTLLTRGAKSDLLSAETAAQMMQRGPKAKLVEFAGVGHAPMFMH
                     DDQIGVVREFLLNG"
     gene            1745..>1881
                     /locus_tag="AC16HB_15980"
     CDS             1745..>1881
                     /locus_tag="AC16HB_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608339.1"
                     /translation="MLKGRTALVTGSTSGIGLAIARALAADGANIVLNGFGDEATIAG
                     I"
BASE COUNT          302 a          640 c          622 g          317 t
ORIGIN      
        1 tcgacgcagt tcgagcactg ggaccgcctg gcctttcaca tggcgccacc gctgctggtg
       61 cggccgggtc cggacggacg gccgcgcaag atcgagttcg gaccctggct gcatggcgcc
      121 ctgcgcatgc tggccaagct caggtggatg cgcgggaccg cgctggatgt gttcggccgc
      181 agcgaagagc gccgcctcga gcgccaattg cgcgacgact acgaggcgat gatcgacggg
      241 ttgttgtcag cgctgaggcc cgacaatcat gcgctcgcgc agcagatcgc ctcggtgccg
      301 gagcagatcc gcggcttcgg ccacgtgaag ctggcgaaca tcaccctcgc gcgcgcgcgc
      361 tggaaggatc tgctcgaccg cttccacgga cgtgccccgg ccgcgcagcc cgttgtggtg
      421 cagttgcagc gcgaacgcgc cggctagggc agcgctgcgg tcaccacgat ctcgaccagc
      481 cagccgggcc gcgccagggt cgcctcgacg gtggcgcgcg ccggcgtgtt gccggggctg
      541 acccaagcgt cccacaccga gttcatcgcc gcgaaatcgg ccatgtcggc gaggaagatg
      601 cttgccgaca gcagacgcga cttgtcggtg cccgcatccg ccagcagctg gtcgatcgcc
      661 gccagcacct gcttcgtctg ccctgccgca tcctgcttgg cgtcgtcggc cacttgcccg
      721 gcgaggaaaa ccatgccgtt gcagatcacg gcttcgctca tgcgcgggcc gacgtgtagt
      781 cgcttgatcg tcatcgtcct atccgttcag aaggaattcg cgcacgacgc cgatctggtc
      841 gtcgtgcatg aacatcggcg cgtggccgac accggcgaac tccaccagct tcgccttcgg
      901 tccgcgttgc atcatctgcg ccgcagtctc ggccgacagc aggtccgact tcgccccgcg
      961 agtcaacagc gtcgggcaac ggatgttgtc ccacagcgcc caggtcgccg cttccgcctg
     1021 cgccgcgatc tccggcgtga cggacttgaa cggctcggcg atgcgcgggt catagtgcag
     1081 tacccagccg tcgccgtcgc ggcggatgac ggtttcagtg atttcgcgcc attcctcgcg
     1141 cgtcctcagc gcgaacggcg caccgaccaa ggcgatgtaa tcggtcgccg cgtcgatgct
     1201 gtcgaagcgg atcggcttgc cgaggtactc gccgatgcgc tggatcgcgg gcgcgctgag
     1261 cgtcggaccg acgtcgttga tcacgaattt cgtcaccggc gaggccggct ggccagcgac
     1321 cgtgatgccg atcagaccgc ccatcgaggt gccgatccag tgcaccgact cgacgttcaa
     1381 ccgcgcgatc agcgtgacca tgtcggacgc atactggccg acgtgatagt ggcgcgggtc
     1441 gcgcagccag tcgctcgcgc cgcgcccggc cacgtcgggg cacaccacgc gataacgatc
     1501 gaacatcgcg cgcgcgaggc ggtcgaagtc tcgtcccacc cgcgtcaggc catgcacgca
     1561 caacagaacg ttcgcgttgc cggggtcgcc ccattccgtg tacgccatcc ggtgaaaacc
     1621 ggcgggcgac aggcacttga ccgacgagaa tcgcggtgat tcgaacggca tctctgctcc
     1681 tttgcgacgc ccattgtagt ttcgtcgcgc tggcatactg gttcccttac atgaggaggt
     1741 cgcaatgctg aaaggcagga ctgcgctggt taccggttcg accagcggaa tcgggctggc
     1801 gattgcgcgc gccctggcgg cggacggcgc caatatcgtg ctgaacggct ttggcgatga
     1861 ggcgaccatc gccggcatcg a
//
LOCUS       DBNHBY010000671         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_523280, whole genome shotgun sequence.
ACCESSION   DBNHBY010000671 DBNHBY010000000
VERSION     DBNHBY010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_523280"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="AC16HB_15985"
     CDS             <1..711
                     /locus_tag="AC16HB_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608340.1"
                     /translation="NGDANTWVHPLLWGYGGKVVDANNKVVLDSPETLKALEYTKELY
                     ATFVPGTLSWLDPNNNKAFLDGQISLTNNGISIYYAAKTSKDPKLQEMAADINHANMP
                     IGPVGKKTEFQLFFNQMIFKYTKYPKAAKEFLRFMMEEEQMNPWVQAAIGYVTPALQA
                     YEKHPIWTSDPKHTPYRDSMKNMLPSGYAGKMGYASAGTLADFVVVDMFAEAASGQRT
                     PKEAAERAQKRAERYYKV"
     gene            726..1640
                     /locus_tag="AC16HB_15990"
     CDS             726..1640
                     /locus_tag="AC16HB_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5608341.1"
                     /translation="MANDGALARALDSRNVLGLLFLVPAALLLLVFLTYPLALGTWLG
                     FTDAKIGRPGVWIGLENYEFLFGDSVARLSLFNTLFYTVVASVFKFALGLWLALLLNK
                     HLPFKSFIRAIVLLPFIVPTALSAIAFWWIYDSQFSIISWTLEKFGLIDRYIDFLGEP
                     WLARMSTIAANIWRGVPFVAICLLAGLQTISPSYYEAASLDGATPWQQFRFVTLPLLT
                     PIIAVVMTFSVLFTFTDFQLIYVLTRGGPLNATHLMATLSFQRAISGGALGEGAALAT
                     AMVPFLLAAILFSYFGLQRRAWQQGGRD"
BASE COUNT          295 a          546 c          542 g          306 t
ORIGIN      
        1 aacggcgatg cgaacacctg ggttcacccg ctgctctggg gttacggcgg caaggtggtc
       61 gacgccaaca acaaggtcgt cctcgacagc cccgagacct tgaaggcgct cgaatatacg
      121 aaggaactgt acgcaacgtt cgtccccggc acgctgtcct ggctcgatcc gaacaacaac
      181 aaggcgttcc tcgacggcca gatctcgctc acgaacaacg gcatctcgat ctactacgcg
      241 gccaagacct ccaaggatcc gaagctgcag gagatggcgg ccgacatcaa ccacgccaac
      301 atgccgatcg ggccggtcgg caagaagacc gagttccagc tgttcttcaa ccagatgatc
      361 ttcaagtaca ccaagtaccc gaaggcggcc aaggagttcc tgcgcttcat gatggaggaa
      421 gagcagatga acccatgggt gcaggcggcg atcggctacg tgacgccggc gctgcaggcg
      481 tacgagaagc acccgatctg gacctcggat ccgaagcaca cgccgtaccg cgactcgatg
      541 aagaacatgc tgccgagcgg ctatgccggc aagatgggct acgcgtcggc gggcacgctg
      601 gccgacttcg tcgtggtcga catgttcgcc gaggcagcaa gcgggcagcg cacgccgaag
      661 gaagccgcgg agcgcgcgca gaaacgcgcc gagcgctact acaaggtctg atcgaggccg
      721 gctgcatggc caacgacgga gcgctcgcgc gggcgctcga cagccgcaac gtcctcgggc
      781 tcctgttcct ggtgcccgcg gcgttgctgc tgctggtgtt tctgacctat ccgctggcgc
      841 tgggcacgtg gctcggcttc accgacgcca agatcgggcg gccgggtgtc tggatcggcc
      901 tggagaacta tgagttcctg ttcggcgaca gcgtggcgcg gctgtcgctg ttcaatacgc
      961 tcttctacac ggtggtggcg agcgtgttca agttcgcgct cggtctgtgg ctggcgctgc
     1021 tgctgaacaa gcatctgccg ttcaagtcgt tcatccgtgc gatcgtgctg ctgccgttca
     1081 tcgtgcccac ggcgctctcc gcgatcgcgt tctggtggat ctacgactcg cagttctcga
     1141 tcatcagctg gacgctggag aagttcggcc tgatcgaccg ttacatcgat ttcctcggcg
     1201 aaccctggct ggcgcgcatg tcgacgatcg ccgccaacat ctggcgcggc gtcccgttcg
     1261 tcgcgatctg cctgctcgcc ggcctgcaga ccatttcgcc gtcgtattac gaggcggcga
     1321 gcctcgacgg cgccacgccc tggcagcagt tccgcttcgt gacgctgccg ctgctgacgc
     1381 cgatcatcgc ggtggtgatg acgttctcgg tgctgttcac gttcaccgac ttccagctga
     1441 tctacgtgct gacgcgcggc gggccgctga acgccacgca cctgatggcg acgctgtcgt
     1501 tccagcgcgc gatctccggc ggcgcgctcg gcgaaggcgc ggcgcttgcc acagcgatgg
     1561 tgccgttcct gctggcggcg atcctgttct cgtacttcgg cctgcagcgc cgcgcgtggc
     1621 agcagggcgg gagggactga tgagcaccgt cgcgtccacc gaaccgacgg tcgacaccca
     1681 gggcatgaa
//
LOCUS       DBNHBY010000672         3136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_725916, whole genome shotgun sequence.
ACCESSION   DBNHBY010000672 DBNHBY010000000
VERSION     DBNHBY010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_725916"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="AC16HB_15995"
     CDS             <1..386
                     /locus_tag="AC16HB_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HIR5608342.1"
                     /translation="VFLFGLTRAPAAQPRALFGGHLGGALIGIACYQLLGDSLWVYAL
                     AQVLALWLMLLTKTVHPPAGANPLIMIHGHAGFSALWQPVLLGVLALAAVAAVWSRLY
                     PGLVHYPVAWLDRLPPSTFWGGWGD"
     gene            577..1377
                     /locus_tag="AC16HB_16000"
     CDS             577..1377
                     /locus_tag="AC16HB_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5608343.1"
                     /translation="MTPTLSRSGALGELGRALVRWLAGWWRLIHLGAQMLVLALSPST
                     YGLANRAALARNIYLDTAPSVLWFAVLSALISLVLIRIVVVTAESYGLTQYALEMVVR
                     VLVLELIPLTAAVFAAVRVTLPNGTEVAALRARGGFEDLRIRGLDPLAHEVLPRVVSG
                     LFCVLMLAAVASVLTLVLAYLHVYGFTAEGLATYTRQVGRVFNAPVTMIFALKTFFLA
                     LAVALIPVATALDDTPRRARRSVELQGLIRLFLVILLIEAVSLVGNYY"
     gene            1377..2333
                     /locus_tag="AC16HB_16005"
     CDS             1377..2333
                     /locus_tag="AC16HB_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5608344.1"
                     /translation="MNAPPSSPQPPVAHVEFKAALLLILLLVLLAGSTLYVMYARGVF
                     EATQELVLVADDSEGVRPGMDLTFSGFPIGRVRKIELAPDGAARIVIDIPRKDAHWLR
                     QSSVFTLTRGLVGGTSIKAFTGVPDDPPLPQGAVRKVLVGDASAEIPRLVATAKELVQ
                     NLTTMTAPDSPLNASLANAQAVSARLKERGALEVLFGNEADARKVVATLDRTNATLAR
                     LNAMADKADAQVFGADGVMNEARTTIVELRAALADTRATLKRVDALLEETQAIAVNTR
                     AATTDLGALRTEVDVNLRKVERLVDELNRKWPFARDTELKLP"
     gene            2330..3052
                     /locus_tag="AC16HB_16010"
     CDS             2330..3052
                     /locus_tag="AC16HB_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608345.1"
                     /translation="MSPPGRPKGESLSAQREGNPASARRIGIAAVLAALLAACASGPP
                     PPDWQASAKDSLERAVAAYMKGDARVAEAEFDRARDEIARTGRPDLLARAELTRCAAR
                     VASLVFEPCAGFEPLRPDAPPAERAYADYLAARIAPTDIALLPPAQRDAAAASAETAG
                     AAVQAIGDPLSRLVAAGVLLQTGRASPAVISVATDTASSQGWRRPLLAWLHVQRLRAE
                     KAGDDGEAERLRRRIRIVEQGR"
BASE COUNT          406 a         1116 c         1119 g          495 t
ORIGIN      
        1 cggtgttcct gttcggtctg acgcgcgcgc ccgccgcgca gccgcgcgcg cttttcggcg
       61 gccacctcgg tggcgcgttg atcggcattg cctgctatca gttgctgggc gacagcctct
      121 gggtctacgc gctcgcgcag gtgcttgcgc tctggctgat gctgctgacg aagaccgtgc
      181 accctccagc cggcgccaat ccgctgatca tgatccacgg tcacgccggt ttctcggccc
      241 tgtggcagcc cgtacttctc ggcgtgctgg cgcttgccgc ggtcgcggcg gtgtggagtc
      301 gcctgtatcc gggccttgtg cactacccgg tcgcgtggct cgaccgcttg ccgccgtcga
      361 cattctgggg cggctggggc gattgagccc gcgcgccgcc cacgaacgcg cgcagaggaa
      421 cctgggctgg agcgcatcag gccgcgcaag gagcggtccg tcttgcgtgc gttgggcgac
      481 aaagaggccc gtgcgcgcga ctattccagc gcatcccccg cccgggtacg agccgaggtc
      541 gatcggcgtg gcgctgcgtc cctctatcat cccggcatga cgccgacgct ttcgcgctcc
      601 ggggcgctgg gtgaactggg ccgcgcgctc gtgcgctggc tggccggctg gtggcgactg
      661 atccatctcg gcgcgcagat gctggtgctg gccctgtcgc cgtcgaccta cggcctcgcc
      721 aaccgcgcgg cgctggcccg caacatctac ctcgacaccg cgccgagcgt gctgtggttc
      781 gcggtgctgt cggcgctgat cagcctggtg ctgatccgca tcgtggtcgt caccgcggaa
      841 agctacggcc tgacccagta cgcgctcgag atggtcgtgc gcgtgctggt gctcgagctg
      901 attccgctga cggcggccgt gttcgctgcc gtgcgggtga cgctgcccaa cggcaccgag
      961 gtcgcggcgc tgcgcgcgcg cggcggattc gaggacctcc gcatccgcgg gctcgatccg
     1021 ctcgcgcacg aggtgctgcc gcgcgtggtg tccggcctct tttgtgtgct gatgctggcc
     1081 gcggtggcca gcgtgctgac gctggtgctc gcgtacctgc acgtgtacgg cttcaccgcc
     1141 gaggggctcg cgacctacac gcgccaggtc ggtcgcgtgt tcaacgcgcc ggtgacgatg
     1201 atcttcgcac tgaagacgtt ttttctcgcg cttgccgtgg cgctgattcc ggtggccacc
     1261 gcgctcgacg acacgccgcg tcgcgcgcgc cgcagcgtcg agctgcaggg cctgatccgc
     1321 ctgtttctgg tgatcctgct gatcgaggcg gtgtcgctgg tcggcaacta ttactgatga
     1381 acgcgccgcc ctcttcgccg cagccgccgg tcgcgcacgt cgagttcaag gccgcgctgc
     1441 tgctgatcct gctgctggtc ctgctggccg gttcgacgct gtacgtgatg tacgcgcgcg
     1501 gggttttcga ggccacgcag gaactggtcc tggtggccga cgattcggaa ggcgtgcgcc
     1561 ccggcatgga cctcaccttc agcggctttc cgatcgggcg cgtgcgcaag atcgagctcg
     1621 ccccggacgg cgccgcgcgc atcgtgatcg acatcccgcg caaggacgcg cactggctgc
     1681 gccagtcgag tgtgttcacg ctcacgcgcg ggctggtcgg cggcaccagc atcaaggcgt
     1741 tcaccggagt cccggacgat ccgccgctgc cccaaggcgc ggtgcgcaag gtgctcgtcg
     1801 gcgatgccag cgccgagatc ccgcgtctgg tcgccaccgc caaggaactg gtgcagaacc
     1861 tgaccacgat gaccgcgccg gactcgccgc tgaacgcgag cctcgccaac gcacaggcgg
     1921 tcagcgcgcg gctgaaggag cgcggcgcgc tcgaagtgct gttcggcaac gaagccgacg
     1981 ccaggaaagt ggtcgccacg ctcgaccgca ccaacgcgac gctggcgcgg ctgaacgcga
     2041 tggccgacaa ggccgatgcg caggtgttcg gtgccgacgg cgtgatgaac gaggcgcgca
     2101 ccaccatcgt ggaattgcgg gccgcgctcg ccgatacacg cgccacgctc aaacgcgtcg
     2161 acgcgctgct ggaggaaacg caggcgatcg ccgtcaacac gcgcgcggcc acgaccgacc
     2221 tcggcgcgct gcgcaccgag gtcgacgtca acctgcgcaa ggtcgagcgc ctggtcgacg
     2281 agctcaaccg caagtggccg ttcgcgcgcg acacggagct gaagctgcca tgagcccgcc
     2341 gggccgcccc aagggcgaat ccctgagcgc gcagcgcgag ggtaatccgg ccagcgcgcg
     2401 ccggatcggg atcgccgccg ttctggccgc gctgctcgcc gcgtgcgcca gtggcccgcc
     2461 gccgcccgac tggcaggcca gcgcgaagga ctcgctggag cgtgccgtgg ctgcctacat
     2521 gaagggcgat gcgcgcgtgg ccgaggcgga gttcgaccgt gcgcgcgacg agatcgcacg
     2581 caccggccgt cccgacctgc tggcgcgcgc ggaactcacg cgctgcgccg cgcgcgtggc
     2641 gagcctcgtg ttcgagccgt gcgcaggatt cgagccgctg cgccccgacg cgccgccggc
     2701 cgagcgcgcc tacgccgact accttgccgc gcgcatcgcg ccgacggaca tcgcgctgct
     2761 gccgcctgcg cagcgcgatg cggctgcggc cagcgccgag acggcgggcg ccgcagtgca
     2821 ggccatcggc gatccgctgt cgcgcctggt tgcggccggc gtgctgctgc agaccggccg
     2881 cgccagcccg gcggtgatat cggtcgccac cgacaccgcg tcgtcgcagg gctggcgcag
     2941 gccgctgctc gcctggctgc acgtgcagcg cctgcgcgcc gagaaggcgg gcgacgacgg
     3001 cgaggcggaa cgcttgcgcc gacgcatccg catcgtcgag caggggcggt gaccggcgac
     3061 gcaccgcgtg ccccggttca tgcactgacc ggcggcggtc gatgattccc gccgcgcttg
     3121 ctgcgcaccg ttggtt
//
LOCUS       DBNHBY010000673         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_929152, whole genome shotgun sequence.
ACCESSION   DBNHBY010000673 DBNHBY010000000
VERSION     DBNHBY010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_929152"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16HB_16015"
     CDS             complement(<1..204)
                     /locus_tag="AC16HB_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608346.1"
                     /translation="MPDRAPRRDAADDTRAIPGNDLAIDRGNSLIARPAVPIILAMSP
                     SSILAASARRSIRPLPDLLVSQIA"
     gene            240..938
                     /locus_tag="AC16HB_16020"
     CDS             240..938
                     /locus_tag="AC16HB_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR5608347.1"
                     /translation="MEGINWSDLFNAMDKFLLAIVHKNVLLVYVVLAAIFFSETGLVF
                     MAFLPGDSLLFVAGAVAAQQTGLSVWVLIVVVTLAAILGNTVNYLIGSWLGHKVYDGS
                     IRWIDKAALDRTHEFYEKWGGLTLILARFTPVVRSFAPLVAGAGSMEIHKFQFYNVIG
                     ASLWVLSLVGSGYLFGNVPFVKDNLGIILIIGIAAVVVPLVLGAGWRLWRGWTMPDAR
                     PGAVVARKDSDARR"
     gene            1133..2125
                     /locus_tag="AC16HB_16025"
     CDS             1133..2125
                     /locus_tag="AC16HB_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608348.1"
                     /translation="MRTRELLLLATLALFTAGCSTWSPPWRTPPAPAAQAQPSAAPED
                     TTDEGVMEKTRESVRSTTVWLARGVDSWFGDIPFEKGGRVTDGRLSFGVLTRQVEGTD
                     VNVRFNARVRLPNVERQAYAFVGRDNQREVIADTPGALTRQQRLLEERPEDQSFFAGL
                     GLPLTDVFDFRAGVRGGLKPYAQARYREPWHLSERDLLEFRETVFWALSDRFGSTTAL
                     SYQHAVSATLGIRWLNAASITQASRNFEWSSVLGAYRNFGDERLLSLEALIIGKSGTG
                     VSLSDYGVQTKWEQRIYRDWLFGEFIVGHFWPRKDAASVREPSWAFGANVKMRF"
     gene            complement(2129..3454)
                     /locus_tag="AC16HB_16030"
     CDS             complement(2129..3454)
                     /locus_tag="AC16HB_16030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR5608349.1"
                     /translation="MPERGDRTLEAHRRRWLKGAAGSLVLSLSPLQIARGATLIGVRV
                     WPAADYTRVTIEHDAPLKFTQFMVRDTPPLRLVVDIEGIDLTPALREIVGRVDANDPY
                     IQLVRVGQNRPRVVRLVVELKEDVKPQVFALEPIGPYQHRLVMDLYPVTPIDPLLALL
                     REQEARGRNESQPGDEQAIEKQIDRSLAPVERAPRPEAGRRDDRVNRFVTVAIDPGHG
                     GEDPGAVGRRGTHEKAVTLAIARRIEAIMRDHANMRVLLTRDGDYFVPLSQRVAKARR
                     VQADLFVSVHADAWIRPDARGSSVFALSERGATSSAAAWIARRENEADQIGGINLGAH
                     DPQIARVLLDLSTTAQISDSLKFGNAVLRELERINRLHKPRVEQAGFAVLKAPDIPSI
                     LVETAFISNPDEELRLRDERYQQQLAQAIVGGVRRYFAKNPPAARNPMG"
     gene            complement(3403..3936)
                     /gene="tsaE"
                     /locus_tag="AC16HB_16035"
     CDS             complement(3403..3936)
                     /gene="tsaE"
                     /locus_tag="AC16HB_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5608350.1"
                     /translation="MHCSAPMPLSQTKLFLADEAATARLGAAFAHAIEHERASIAAHG
                     LVIGLSGDIGAGKTSLVRALLRALGVTGPVKSPTFALLEPYTVSSLDFYHFDFYRFTD
                     PREFDAAGFRELFGPGAVCAIEWPERAGVLPTPDLALTLRVAEDGRRVHLVAHSELGI
                     ACLNEAIARWKHTAAAG"
     gene            3939..>4573
                     /gene="queG"
                     /locus_tag="AC16HB_16040"
     CDS             3939..>4573
                     /gene="queG"
                     /locus_tag="AC16HB_16040"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR5608351.1"
                     /translation="MQVKDTEQVLDLAQLAQDLKAWGRELGFAAIGISDVDTGTAPPR
                     LRAWLDAGRHGEMDYMERHAHLRADPSLLLPRAARVISARIDYLPRDDDSQWAEREQR
                     RLHEGSAAVVSIYARGRDYHKVLRDRLQKLATRIESKIGPFGYRACTDTAPVFEIEFA
                     RKAGLGWRGKHTLLLAQDAGSMFFLGEILTDLPLPVDAPTKEHCGTCERCI"
BASE COUNT          714 a         1521 c         1586 g          752 t
ORIGIN      
        1 ggcgatctgg ctcaccagca ggtcgggcag gggtcggatc gagcggcgcg cgctcgccgc
       61 gaggatggaa gagggcgaca tcgcaaggat tataggaacc gcgggccgcg cgatcagtga
      121 attccctcgg tcgatcgcga ggtcgttgcc cgggatcgct cgcgtatcat cggccgcatc
      181 tcggcggggt gcccgatcgg gcactccgcc atctttttgt tcacggcccg cgcatcgcga
      241 tggaaggcat caactggtcc gacctgttca acgcgatgga caagttcctg cttgccatcg
      301 tgcacaagaa cgtgctgctg gtgtacgtgg tgctggcggc gatcttcttt tccgagaccg
      361 ggctcgtctt catggcgttt ctgccgggcg actcgctgct gttcgtcgcc ggcgcggtgg
      421 cggcgcagca gacggggctg tcggtgtggg tgctgatcgt tgtcgtcacg ctggccgcga
      481 ttctcggcaa caccgtcaac tacctcattg gctcctggct cggccacaag gtctacgacg
      541 gcagcatccg atggatcgac aaggcggcgc tcgaccgcac gcacgagttc tacgagaagt
      601 ggggcgggct gacgctgatc ctcgcgcgct tcacgccggt ggtgcgctcg ttcgcgccgc
      661 tggtggccgg cgccggcagc atggagatcc acaagttcca gttctataac gtgatcggtg
      721 cgtcgctgtg ggtgctgtcg ctggtcggca gcggctatct gttcggcaac gtgccgttcg
      781 tgaaggacaa cctcggcatc atcctgatca tcggcatcgc ggcggtggtg gtgccgctgg
      841 tgctcggcgc cggctggcgc ctgtggcgcg gctggacgat gccggacgcg cgccccggcg
      901 cggtcgtggc ccgcaaggac agcgacgcac ggcgctgaat gccgcgcgcg ctctgcgccg
      961 ggttcaatgc tggaggcatc agatcaccgg cggagcgccg gagacacgac ggctgtggca
     1021 atccgcggcg acagcgcgcg ggtgcgggac tcagctcttc tcgcaccgag cggcatcgtc
     1081 cgtacaccgc gctgccggcc gcggcgttag ctgatcatca tcgatgaggc gtatgcgaac
     1141 ccgggaactg ctgctgctcg cgacgctggc gctgttcacg gccgggtgca gcacgtggtc
     1201 gcctccgtgg cgaactcctc cggcgcccgc cgcacaagcg cagcccagcg ctgcgcccga
     1261 ggacacgacg gacgaaggcg tgatggagaa gacgcgcgag tccgtgcgct cgacgaccgt
     1321 ctggctcgcg cgcggcgtgg acagctggtt cggcgacatc ccgttcgaga agggcggtcg
     1381 tgtgacggac ggccggctga gcttcggcgt gctcacgcgt caggtcgaag gcaccgacgt
     1441 gaacgtgcgc ttcaacgcgc gcgtgcgcct gcccaacgtc gagcggcagg cgtacgcgtt
     1501 cgtcggccgc gacaaccagc gcgaggtcat cgccgatacg cccggcgcgc tgacgcgcca
     1561 gcaacggctg ctcgaggaac ggcccgagga tcagtcgttc ttcgccggcc tcggcctgcc
     1621 actgacggac gtgttcgact ttcgcgccgg cgtgcgcggc gggctgaagc cctacgcgca
     1681 ggcgcgctat cgcgagccgt ggcacctgtc cgagcgcgat ctgctcgagt ttcgcgaaac
     1741 cgtgttctgg gcgctgtccg accgcttcgg ctcgaccacc gcgctgtcgt accagcatgc
     1801 ggtgtcggcg acgctcggca tccgctggct caacgcggcg tcgatcacgc aggcgtcgcg
     1861 caacttcgaa tggtcgagcg tgctcggcgc gtaccgcaac ttcggcgacg agcgcctgct
     1921 gtcgctcgag gcgctgatca tcggcaagtc gggcacgggc gtgtcgctct ccgactatgg
     1981 cgtgcagacg aagtgggagc agcgcatcta cagggactgg ctgttcggcg aattcatcgt
     2041 cggccacttc tggccgcgca aggacgcggc gagcgtgcgc gaaccgagct gggctttcgg
     2101 cgccaacgtg aagatgcgct tctagggcct agcccatcgg attgcgcgcg gccggcggat
     2161 tcttcgcgaa gtagcggcgt acgccgccga cgatcgcctg cgcgagttgc tgttgatagc
     2221 gctcgtcacg cagccgcagt tcctcgtcgg gattgctgat gaaagcggtc tcgaccagga
     2281 tcgacggaat gtcgggcgcc ttgagcaccg cgaagccggc ttgctcgacg cgcggcttgt
     2341 gcaggcggtt gatgcgctcg agctcgcgca gcaccgcatt gccgaacttc aggctgtcgc
     2401 tgatctgcgc cgtggtggac agatcgagca gcacgcgcgc gatctgcgga tcgtgcgcgc
     2461 cgagattgat gccgccgatc tggtcggcct cgttctcgcg tcgcgcgatc cacgccgccg
     2521 cgctgctggt ggcgccgcgc tccgacagcg caaacaccga cgagccgcgc gcgtccggcc
     2581 ggatccaggc atcggcgtgc accgacacga acaggtccgc ctgcacgcgc cgcgccttgg
     2641 ccacccgctg gctcagcggc acgaagtagt cgccatcgcg cgtcagcagc acgcgcatgt
     2701 tggcgtggtc gcgcatgatc gcctcgatgc ggcgcgcgat cgccagcgtc accgccttct
     2761 cgtgcgtgcc gcgccggccg accgcgccgg ggtcctcgcc gccgtggccg ggatcgatcg
     2821 caacggtgac gaagcggttg acgcgatcgt cgcgccggcc cgcctcgggg cgcggcgcac
     2881 gttcgacggg cgccagcgag cggtcgatct gcttctcgat tgcctgttcg tcgcccggct
     2941 gcgactcgtt gcgcccgcgc gcttcctgct cacgcagcag cgccagcagc ggatcgatcg
     3001 gtgtgaccgg atacaggtcc atcaccagcc ggtgctgata aggaccgatc ggttcgagcg
     3061 cgaagacctg cggcttcacg tcctccttca gctcgaccac cagccgcacg acgcgcggcc
     3121 ggttctggcc cacgcgcacg agctggatgt acgggtcgtt cgcatcgaca cggccgacga
     3181 tctccctgag cgcgggcgtg aggtcgatgc cctcgatgtc gaccaccagt cgcagcggcg
     3241 gtgtgtcgcg caccatgaac tgcgtgaact tcagcggcgc gtcgtgctcg atcgtcacgc
     3301 gcgtgtaatc ggcggccggc catacgcgca cgccgatgag cgtggcacca cgcgcgatct
     3361 gcagcggtga gagcgacaga acgagactgc cggccgcgcc cttcagccag cggcggcggt
     3421 gtgcttccag cgtgcgatcg cctcgttcag gcatgcgatt ccaagctccg agtgggcgac
     3481 gagatgcacg cgccgcccgt cttctgcgac tctcaacgtc agcgcgaggt ctggcgttgg
     3541 caacactccc gcgcgttcgg gccactcgat tgcgcacacg gcgccggggc cgaacaactc
     3601 gcggaacccc gcagcgtcaa actcccgcgg gtccgtgaaa cgataaaaat caaagtgata
     3661 gaagtctaag ctcgaaacgg tataaggttc aagcagcgca aaggtgggac tcttgacggg
     3721 gcccgtcacg ccgagcgcgc gcagcagcgc gcgcaccagc gacgtcttgc ctgcgccgat
     3781 gtcgccactg agcccgatca cgaggccgtg cgcggcgatc gacgcgcgtt cgtgttcgat
     3841 cgcgtgtgcg aacgcagcgc caagccgcgc agtcgcggct tcatcggcga ggaacagctt
     3901 cgtttgcgaa agcggcatcg gcgcagaaca atgcatacat gcaggtgaag gataccgagc
     3961 aggtgctcga tctcgcgcaa ctggcgcagg acctcaaggc gtggggccgc gaactggggt
     4021 tcgcagcgat cggaatcagc gatgtcgaca ccggcacggc gccgccgcga ctgcgtgcat
     4081 ggctcgacgc cggccgccac ggcgagatgg attacatgga acgccatgcc cacctgcgcg
     4141 ccgatccgtc gctgctgctg ccgcgcgcgg cgcgcgtgat cagcgcgcgc atcgactacc
     4201 tgccgcgcga cgacgactct cagtgggccg agcgcgagca acggcgcctg cacgaaggca
     4261 gcgccgcggt ggtgtcgatc tacgcccgcg ggcgcgacta ccacaaggtg ctgcgcgatc
     4321 ggctgcagaa gctcgcaacg aggatcgaaa gcaagatcgg tccgttcggc tatcgcgcgt
     4381 gcaccgatac cgcgccggta ttcgagatcg agtttgcgcg caaggcgggg ctgggctggc
     4441 gcggcaagca cacgctgctg ctggcgcagg acgcgggctc gatgttcttt ctcggcgaga
     4501 tcctgaccga cctgccgctg cccgtcgatg caccgaccaa ggaacattgc ggcacgtgcg
     4561 aacgctgcat cga
//
LOCUS       DBNHBY010000674         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_116957, whole genome shotgun sequence.
ACCESSION   DBNHBY010000674 DBNHBY010000000
VERSION     DBNHBY010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_116957"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="AC16HB_16045"
     CDS             <1..1201
                     /locus_tag="AC16HB_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608352.1"
                     /translation="TPFVDRMVSTIEKERQPLEAKVIQGYYDVPQIERLDDGFKLQQE
                     LRDQTGRWKLLQDHGIQLPTSVEPNSWYMGFNWLDPVVGRGSTPEDEVRHRKLRQAIS
                     IATDWEEYTNIFFDTYGEAETAMSPLPPGVFGYRAGCDGIDPVTHVCRDGRPQRRSLD
                     EAKKLLAEAGYPGGRDARTGKPLVLYYDSNGVGPAYQARLDWQQKQMAKLDIQLEIRS
                     TDYNRFQDRMRKGQAQIFFWGWNADYPDPENFLFLLHSLQSKVKFDGENAANYDNAEY
                     DKLYDEMKNLPDGPQRQAVIDRMVKIVQNDAVWMFGIFPGATGVYQTWVHNAKPALIL
                     KDQAKYLRVDVPLRASRIAQWNQPRPWPVAVFFALAALVLWPAWRLWQRRERTSARGR
                     LIEAGSA"
     gene            1201..2178
                     /locus_tag="AC16HB_16050"
     CDS             1201..2178
                     /locus_tag="AC16HB_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608353.1"
                     /translation="MLAYLARRLLYAVLVLAGVNLLVFILFFTINTPEDMARLQLGGK
                     RVTAEAVERWKAERGYDKPLYFNASAPGLAKLTNTVFVDTTTRLAHFDFGRTNEGRDI
                     AEEIRERLPISFGIGVPVFILTVGVSVVFSLGLAMFRGTKLDAAGVVLLVALMSISSL
                     FFIIMGQYVFSKVLRLAPLSGYVPGPEMFSFVMLPILVLVIARLGPDARLYRTIYLEE
                     LGKDYVRTARSKGLSEFAIMFRHVLRNSWLPIITSSASLLPVVFGGAIVTETFFAIPG
                     LGALTIEGITQQDFSIVRAMVFFGSALYIAAYLMTDVLYAVADPRIRLH"
     gene            2180..3577
                     /locus_tag="AC16HB_16055"
     CDS             2180..3577
                     /locus_tag="AC16HB_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608354.1"
                     /translation="MKPVILWTDWAVLIVLVGAVLYAWRVARTAHLAATWRKAFVRPV
                     GAVTATLLAIVAAIGVIDSVHFRERLPDAGGQVAYSPVTRSLLDQLLVRQEAGRETSY
                     SRPLAYLGYQKETIAVDGKEVRDFPRLKHGGAHLKDPEREWGADIAARTATGLAGGAI
                     ATALLALAVAALRARRLRIAVSASAREIVRGHTVFPVRALLVTFAVLALLIGTIASLA
                     SGYHVFGTDRTGNDLLYLALKSIRTALVIGTMTTLVTLPIAVGLGIAAGYLRGWVDEV
                     IQYLYTVLNSIPYLLLLAAAVLILIIFIDNNPDLFQTAAERTDARLFFLCVILGVTSW
                     TELARLIRAEVLRLREMEYVQAAQAFGVRTWQIMAVHLVPNLMHVILINVVIAFSGLV
                     LAEAILSYVGVGVDPSMASFGTLINTARAELSREPAIWWTLATAFVFMFVIVLAANLF
                     ADAVRDAFDPRFIDR"
     gene            3637..>3859
                     /locus_tag="AC16HB_16060"
     CDS             3637..>3859
                     /locus_tag="AC16HB_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR5608355.1"
                     /translation="MDYAIEFYSEEVAEQILALPDTLAARYVVLTRRMAAVGPNLGPP
                     HTASFGDGLFELRLRGAEGIARVFFCTLIG"
BASE COUNT          595 a         1248 c         1318 g          698 t
ORIGIN      
        1 gacgcccttc gtcgatcgga tggtctcgac gatcgagaag gagcgccagc cgctcgaggc
       61 caaggtgatc cagggctact acgacgtgcc gcagatcgag cgcctcgacg acgggttcaa
      121 gctgcagcag gaactgcgcg accagaccgg ccgctggaag ctgctgcagg atcatggcat
      181 ccagttgccg acgtcggtcg agcccaacag ctggtacatg ggcttcaact ggctcgaccc
      241 ggtggtcggc cgcggctcca cgccggagga cgaggtacgc catcgcaagc tgcgccaggc
      301 gatctcgatc gcgaccgact gggaggaata caccaacatc ttcttcgaca cctacggcga
      361 ggccgagacc gcgatgagcc cgctgccccc gggcgtgttc ggctaccgcg ccggctgtga
      421 cggcatcgat ccggtcacgc atgtgtgcag ggacggccgg ccgcagcgcc gctcgctcga
      481 cgaggcgaag aagctgctgg ccgaggccgg ctaccccggc ggccgcgacg caaggacagg
      541 caagccgctg gtgctgtact acgacagcaa cggcgtcggt ccggcttacc aggcgcggct
      601 cgactggcag cagaagcaga tggcgaagct cgacatccag ctcgagatcc gctcgaccga
      661 ctacaaccgc ttccaggacc gtatgcgcaa ggggcaggcg cagatcttct tctggggctg
      721 gaatgccgac tatcccgatc cggagaactt cctgttcctg ctccacagct tgcagtcgaa
      781 ggtgaagttc gacggcgaga acgcggccaa ctacgacaac gccgaatacg acaagctcta
      841 tgacgaaatg aagaacctgc ccgacggtcc gcagcggcag gcggtgatcg accggatggt
      901 gaagatcgtg cagaacgacg ccgtgtggat gttcggcatc ttccccggcg ccacgggcgt
      961 ctaccagacg tgggtgcaca acgccaagcc ggcgctgatc ctgaaggacc aggcgaagta
     1021 cctgcgcgtc gatgtgccgc tgcgcgcctc gaggatcgcg caatggaacc agccgcgccc
     1081 gtggccggtg gccgtgttct tcgcgctcgc ggcgctcgtg ctgtggcccg cttggcggct
     1141 gtggcagcgg cgcgagcgca cctcggcgcg cggacgactg atcgaggcgg gctcggcctg
     1201 atgctcgcct acctcgcgcg ccggctgctg tatgcagtgc tcgtgctggc gggcgtgaat
     1261 ctgctcgtgt tcatcctgtt cttcaccatc aacacgccgg aggacatggc gcggctgcaa
     1321 ctgggcggca agcgcgtgac ggccgaggcc gtcgagcgct ggaaggccga gcgcggttac
     1381 gacaagccgc tttacttcaa cgcgagcgcg ccggggctgg cgaagctgac gaacacggtt
     1441 ttcgtcgaca cgaccacgcg gctcgcgcac ttcgacttcg gcaggaccaa cgaagggcgc
     1501 gacatcgccg aggagatccg cgagcggctg ccgatctcgt tcggcatcgg cgtgccggtg
     1561 ttcatcctga cggtcggcgt gtcggtcgtg ttctcgctcg ggctggcgat gttccgcggc
     1621 acgaagctcg acgccgccgg cgtggtgctg ctggtggcgc tgatgtcgat ctcgtcgctg
     1681 ttcttcatca tcatgggcca gtacgtgttc agcaaggtgc tgcggctggc gccgctgtcc
     1741 ggctacgtgc cgggcccgga gatgttcagc ttcgtcatgc tgccgatcct ggttctggtg
     1801 atcgcgcggc tcggtcccga cgcgcggctg taccgcacga tctatctcga ggagctcggc
     1861 aaggactacg tgcgcaccgc gcgcagcaag gggctctcgg agttcgcgat catgttccgg
     1921 cacgtgctgc gcaattcgtg gcttccgatc atcacgtcgt cggcttcgct gctgccggtg
     1981 gtcttcggcg gcgcgatcgt gaccgagacc ttcttcgcca tcccgggcct cggggcgctg
     2041 acgatcgagg gcatcacgca gcaggatttc tcgatcgtgc gcgcgatggt gttcttcggc
     2101 tcggcgctgt acatcgccgc ctacctgatg accgatgtgc tgtacgcggt cgccgatccg
     2161 cgcattcggt tgcattgaaa tgaaaccggt gatcctgtgg accgactggg cggtcctgat
     2221 cgtgctggtg ggtgccgtgc tctacgcatg gcgcgtggcg cgcacggcgc acctggcggc
     2281 gacgtggcgc aaggctttcg tgcggccggt cggcgccgtg accgcgacgc tgctggcaat
     2341 cgtggccgcg atcggcgtga tcgattcggt gcactttcgc gagcgcttgc ccgacgccgg
     2401 ggggcaggtc gcgtactcgc cggtcacgcg ctcgctgctc gatcagctgc tggtgcggca
     2461 ggaagcggga cgcgagacct cgtattcacg gccgctggcg tacctcggct accagaagga
     2521 gacgatcgca gtcgacggca aggaggtgcg cgattttccg cggctcaagc acggcggcgc
     2581 gcacctgaag gacccggagc gcgagtgggg cgcggatatc gcggctcgaa cggcgacggg
     2641 actggccggc ggcgcgatcg ccacggcgct gctggcgctg gcggtggcgg ccctgcgcgc
     2701 gcgccggctt cgcattgcgg tatcggcatc ggcgcgcgag atcgtgcgcg gccacaccgt
     2761 gtttccggtg cgcgcgctgc tcgtcacgtt cgccgtgctc gccctgctga tcggaacgat
     2821 cgcatcgctg gcgtccggct atcacgtgtt cggcaccgac cgcaccggca acgacctgct
     2881 gtatctcgcg ctgaagagca ttcgcaccgc gctggtgatc ggcacgatga cgacgctcgt
     2941 cacactgccg atcgcggtcg ggctcggcat cgcggccggc tacctgcgcg gctgggtcga
     3001 cgaggtgatc cagtacctgt acacggtgct caactcgatt ccgtacctgc tgctgctcgc
     3061 cgcggccgtg ctgatcctga tcatcttcat cgacaacaac cccgacctgt tccagaccgc
     3121 ggccgagcgc accgacgcgc ggctgttctt cctgtgcgtg atcctcggcg tcacgagctg
     3181 gaccgaactg gcgcgcctga tccgcgccga ggtgctgcga ctgcgcgaga tggagtacgt
     3241 gcaggcggcg caggccttcg gcgtccgcac ctggcagatc atggccgtgc acctggtgcc
     3301 aaacctgatg cacgtgatcc tgatcaacgt cgtgatcgcg ttctccggac tcgtgctggc
     3361 ggaggcgatc ctgtcgtatg tcggcgtcgg agtcgacccg tcgatggcga gcttcggcac
     3421 gctgatcaac accgcgcgcg cggagctgtc gcgcgagccg gccatctggt ggacgctggc
     3481 cacggcgttc gtcttcatgt tcgtgatcgt gctggcggca aatctcttcg ccgacgcggt
     3541 gcgcgatgcg ttcgacccgc gcttcatcga caggtgatgc tgtgccggcg ccagccgacg
     3601 ttggggtcta agtaacatat ttgttataat acgcgcgtgg actacgccat cgagttctac
     3661 agcgaagagg ttgcggagca aatcctcgcg ctgcccgata cgctcgcggc tcgatatgtg
     3721 gttctgactc gccgcatggc cgcggtgggt ccaaatctcg gcccgccgca caccgcgtcc
     3781 tttggcgatg gtttgttcga gctgcgactc aggggcgccg agggaatcgc tcgcgtcttc
     3841 ttctgcacgc tgatcggcc
//
LOCUS       DBNHBY010000675         5675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_523460, whole genome shotgun sequence.
ACCESSION   DBNHBY010000675 DBNHBY010000000
VERSION     DBNHBY010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_523460"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16HB_16065"
     CDS             complement(<1..313)
                     /locus_tag="AC16HB_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5608356.1"
                     /translation="MSGRAAAVANPEPGVIEAVRRRVATAFDPGGDLAGSVRGFVPRA
                     SQREMALRIFDAITERGTFVAEAGTGTGKTFAYLVPSLLAGGKVLVSTGTKSLQDQIF
                     RK"
     gene            complement(310..507)
                     /locus_tag="AC16HB_16070"
     CDS             complement(310..507)
                     /locus_tag="AC16HB_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR5608357.1"
                     /translation="MESDEIKRRIIELQVEHRDLDEAIARLADQPGIDEIRLRRMKKR
                     KLQIKDAIYMLQMQLVPDIPA"
     gene            621..1451
                     /locus_tag="AC16HB_16075"
     CDS             621..1451
                     /locus_tag="AC16HB_16075"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5608358.1"
                     /translation="MSRFKVTACTAEHIGDRAEQQDRVALITSKRNPNALLAVVADGM
                     GGRSGGRMASDQVISTADHVFQEIGESDAGGLRALLEQIATEAHTVIRLTAISSEQEP
                     HATIVALIVRKDYAIWAHAGDSRLYLFRGGRLLHRTSDQTYAAHLEAEGKAQEAARAA
                     RHLKHVLVSALGVTRPLTLVIDEIDHLRVGDAFLLCSDGVWAHFEDQELGSLLHSLTP
                     REASEHIVRIARGRAQSRGDNLALAIVKLEAPEPTKRLPTATLWCDDEPARNGGDKPA
                     "
     gene            complement(1455..2117)
                     /locus_tag="AC16HB_16080"
     CDS             complement(1455..2117)
                     /locus_tag="AC16HB_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608359.1"
                     /translation="MKHALWVVALIAVPAFAADDPIARAYPPGSIGTRDQAAGATQAA
                     RARTEEIEREFKLESAHCETVVLVTGCRSDARRKRDARLREVERVRLEARDMTRRLDA
                     QERAQARAADAARRAEQAAIKAEQAAQSRAAFEARQRAAERRQPKAPEPRGPKAAPAP
                     NEPSARERAENVRRLQEKQRAAAEYAQRKAKEREENARRREQRRLEREAEAKRRAAAA
                     SK"
     gene            complement(2114..3208)
                     /gene="zapE"
                     /locus_tag="AC16HB_16085"
     CDS             complement(2114..3208)
                     /gene="zapE"
                     /locus_tag="AC16HB_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR5608360.1"
                     /translation="MSFRSHLDALLAERGYTLDSSQQRAAERLAGLDADWIAYKHARR
                     NAFLKLVSRPGIPRGVYLYGGVGRGKSFLMDCFFSAVPLIRKTRIHFHEFMRGVHREL
                     DELKGTQDPLDEVARRVAHRYRLICFDEFHVSDIADAMILHRLLVRLFDLGVGFVMTS
                     NYAPAALYPDGLHRDRFVPTIELLLRELDVLQIDAGVDYRERALERIDVYLTPLDTRS
                     DARMQAAFRGIAETEDDDPVLLIEARQIRARLRAGGVVWFEFATLCGGPRSQNDYLEI
                     AAQFHTVLLSNVPQMSSGQSSEARRFTWLVDVLYDHGVKLVLSAATRPELLYPSGALA
                     AEFKRTVSRLIEMQSKRYLEAPRRAVAARL"
     gene            complement(3211..4629)
                     /gene="lpdA"
                     /locus_tag="AC16HB_16090"
     CDS             complement(3211..4629)
                     /gene="lpdA"
                     /locus_tag="AC16HB_16090"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5608361.1"
                     /translation="MSKSFDVVVIGAGPGGYIGAIRAAQLGFSVACVDEWKNAKGGPA
                     PGGTCTNVGCIPSKALLQSSENYEQAAHHFKDHGIEVKGLSMDVAQMLARKDKVVRQN
                     NDGISFLFKKNKIAFFHGRGSFAGPGDGGYRIQVNDETLTAKHVVVATGSNPRALPGA
                     DFDEKLILSNDGALAISEVPGRLGVVGAGVIGLEMGSVWRRLGAEVTVLEALPAFLGA
                     ADESIAKEAQKAFTKQGLKMHFGVNITGVTKAKNAVTVEYADHAGAAQTLACDRLIVS
                     IGRVPNTTGLAPDAVGLKLDERGFVVVDDACRTNLPNVWAIGDVVRGPMLAHKAEEEG
                     VAVAERIAGQHGHVDFNTIPWVIYTSPEIAWVGQTEQQLKATGRAYKSGSFPFSANGR
                     ARALGDTTGLVKFLADAQTDEILGVHIVGPLASELIAEAVVAMEFRASSEDIARICHA
                     HPSLSEVTKEAALAVEKRTLNL"
     gene            complement(4648..>5675)
                     /gene="odhB"
                     /locus_tag="AC16HB_16095"
     CDS             complement(4648..>5675)
                     /gene="odhB"
                     /locus_tag="AC16HB_16095"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR5608362.1"
                     /translation="KTGARAASAPAPVPAAAPSPIGAAAPASGAIAMPAAAKMLADAG
                     MSPAQVAGSGKDGRILKGDVIAAKEGIKAAPAAAPAPTPTPTPTPAARPALPEVKAPL
                     DMRMVSDRPEQRVPMSRLRQRVAERLVQSQSTAAILTTFNEVNMAPLMDLRSKYKDRF
                     EKEHGVKLGFMSFFVKAAVAALKRFPVVNASVDGADIVYHGYFDIGIAVGSPRGLVVP
                     ILRDADQMTFADIEKKIADFGKRAGEGKLAIEELTGGTFSISNGGVFGSMLSTPIINP
                     PQSAILGIHATKDRPVVENGQIVIRPVNFLALSYDHRIIDGREAVLSLVAMKEALEDP
                     ARLLLEV"
BASE COUNT          869 a         2007 c         1868 g          931 t
ORIGIN      
        1 ccttgcggaa gatctgatcc tgcagcgact tggtgccggt cgagacgagc accttgccac
       61 ccgcgagcag tgacggcacg aggtacgcga acgtcttgcc ggtgcccgtg ccggcttctg
      121 cgacgaatgt gccgcgctcc gtgatcgcat cgaagatgcg cagcgccatt tcgcgctggg
      181 acgcgcgcgg cacaaagccc cgcaccgacc cggcaagatc gccgccggga tcgaacgccg
      241 tcgcgacgcg gcggcggacc gcctcgatta cgcccggttc cggattggcg accgccgctg
      301 cgcggccact cacgccggaa tgtcgggcac gagctgcatc tgcagcatgt agatcgcgtc
      361 cttgatctgc agtttgcgct tcttcatccg gcgcagacgg atctcgtcga tgcccggctg
      421 atcggccagc cgtgcgattg cctcgtccag atcgcggtgc tcgacctgca attcgatgat
      481 gcggcgcttg atctcgtccg actccatgat cccgccctcc gcgcgactca actgaagcag
      541 catgaataca tgctagcagg gacgctcatc cgttcggcgc gctcggcgcg acggcggcgc
      601 ggctagactc gccgtcctct atgtcgcgct tcaaggtcac ggcctgcact gccgagcaca
      661 tcggcgaccg cgccgagcag caggatcgcg tcgcgctgat caccagcaag cgcaacccca
      721 atgcactgct cgccgtcgtg gccgacggca tgggcgggcg cagcggcggc cgtatggcgt
      781 ccgaccaggt gatcagcacc gccgaccacg ttttccagga gatcggcgaa agcgatgccg
      841 gcgggctgcg cgcgctgctc gagcagatcg ccaccgaggc gcatacggtg atccgcctga
      901 ccgcgatatc gagcgagcag gagccacacg cgaccatcgt cgcgctgatc gtgcgcaagg
      961 actacgcgat ctgggcgcat gccggcgaca gccgactgta cctgttccgc ggcgggcgcc
     1021 tgctgcaccg aacttccgac cagacctacg ccgcgcacct ggaagccgaa ggcaaggcac
     1081 aggaagccgc gcgtgccgcg cgccatctga agcacgtgct ggtgagcgcg ctcggcgtca
     1141 cgcggccact gacgctggtg atcgacgaga tcgaccacct gcgcgtcggc gacgcctttc
     1201 tcctgtgcag cgatggcgta tgggcccatt tcgaggatca ggaactgggc tcgttgctgc
     1261 acagcctgac cccgcgcgag gcgtccgagc acatcgtccg catcgcgcgc gggcgcgcgc
     1321 agtcgcgcgg ggacaacctc gcactggcga tcgtcaagct cgaagcgccc gagccgacca
     1381 agcgcctgcc gaccgccacg ctctggtgcg atgacgaacc ggcccgcaac gggggcgaca
     1441 agcccgcctg agcctcactt cgacgccgcg gccgcgcgcc gcttcgcctc ggcctcgcgc
     1501 tccagacggc gctgctcgcg ccgccgcgca ttctcctccc gctccttggc cttgcgctgc
     1561 gcgtactccg ccgcggcgcg ctgcttttcc tgcagtcggc gcacgttctc ggcgcgctcc
     1621 cgggcactgg gttcattcgg tgccggcgcg gcctttggcc cgcgcggctc cggcgccttc
     1681 ggctgccgtc gttcggcggc gcgctgccgg gcctcgaacg cggcgcgcga ctgggccgcc
     1741 tgttccgcct tgatcgctgc ctgctccgcg cgtcgcgcgg catcggcagc ccgcgcctgc
     1801 gcgcgctcct gcgcgtcgag ccggcgcgtc atgtcgcgcg cctcgagccg cacgcgctcg
     1861 acttcgcgca gccgggcgtc gcgcttgcgg cgcgcatcgg accggcaacc ggtgacgagc
     1921 acgaccgttt cgcagtgcgc cgactcgagc ttgaactcac gctcgatctc ctcggtgcgc
     1981 gcgcgggccg cctgcgtcgc gcccgcagcc tgatcccgag tgccgatcga gcccggggga
     2041 taagcgcgcg cgatcggatc gtccgccgcg aacgcgggca cggcgatcag cgcgacgacc
     2101 cacagcgcgt gcttcacagt cgtgccgcga ccgcacggcg cggtgcttcc agatagcgct
     2161 tcgactgcat ctcgatcagc ctggataccg tgcgcttgaa ttccgccgcc agcgcaccgg
     2221 atgggtacag cagttccggc cgcgtcgccg ccgacagcac gagcttcacg ccgtggtcgt
     2281 acagaacgtc gaccagccac gtgaagcggc gcgcctcgct cgactggccg gacgacatct
     2341 gcggcacgtt ggaaagcagg acggtatgga actgggcggc gatttccaga tagtcgttct
     2401 gcgagcgcgg accaccgcaa agcgtcgcga actcgaacca gaccacgccg ccagcccgga
     2461 gccgcgcgcg aatctggcgc gcctcgatca gcagcaccgg atcgtcgtcc tcggtctccg
     2521 cgatgccgcg aaacgccgcc tgcatgcgcg cgtccgaccg tgtgtcgagc ggcgtcaggt
     2581 agacgtcgat tctctccagc gcgcgctcgc ggtagtcgac gccggcgtcg atctggagca
     2641 cgtcgagttc gcgcagcagc agttcgatcg tcggcacgaa gcggtcccgg tgcaggccat
     2701 cggggtatag cgccgccggc gcgtagttgg acgtcatcac gaagccgacg ccgaggtcga
     2761 acagccgcac gagcagccgg tgcaggatca tcgcgtcggc gatgtcggag acgtggaact
     2821 catcgaagca gatcaggcgg taacggtgcg cgacgcggcg cgcaacttcg tccaatgggt
     2881 cctgcgtgcc tttcagttcg tcgagttcac ggtgcacgcc gcgcatgaac tcgtgaaagt
     2941 ggatgcgcgt cttccggatc aggggtaccg ccgagaagaa gcagtccatc aggaagctct
     3001 tgccccgccc gacgccgccg tacagataca cgccgcgggg aatcccgggc cggctgacga
     3061 gcttcagaaa ggcgttgcgg cgcgcgtgct tgtaggcgat ccagtccgcg tcgagccctg
     3121 caagtcgctc ggcagcgcgc tgctgcgaac tgtcgagcgt gtatccgcgc tccgccagca
     3181 gggcatcgag gtgtgagcgg aaggacacgc ctacagattc agcgtccgtt tctccaccgc
     3241 cagcgccgcc tccttggtca cttccgacag cgacggatgg gcatggcaga tgcgagcgat
     3301 gtcctcgctg gaggcgcgga actccatcgc gaccaccgcc tcggcgatga gttcggacgc
     3361 cagcgggccg acgatgtgca ccccgaggat ctcgtcggtc tgggcgtcgg ccaggaactt
     3421 gaccaggccg gtcgtatctc ccagcgcgcg cgcgcggccg ttggccgaga acgggaacga
     3481 acccgacttg tacgcgcggc cggtggcctt cagctgctgc tcggtctgtc cgacccacgc
     3541 gatctcggga gacgtgtaga tcacccacgg gatcgtgttg aagtcaacgt ggccatgctg
     3601 accggcgatg cgctctgcga ctgccacgcc ttcttcctcc gccttgtgcg cgagcatcgg
     3661 gccgcgcacc acgtcgccga tcgcccacac gttcggcaga ttggtgcggc acgcgtcgtc
     3721 gacgaccacg aagccgcgct cgtccagctt cagccccacg gcgtcgggcg ccagcccggt
     3781 cgtgttcggc acgcggccga tcgacacgat cagccggtcg caagcaagcg tctgcgcggc
     3841 gccggcatgg tcggcgtact cgaccgtcac ggcgttcttc gctttcgtca cgcccgtgat
     3901 gttgacgcca aagtgcatct tcaggccctg cttcgtgaag gccttctgcg cttccttcgc
     3961 aatcgactcg tccgccgcgc cgaggaaggc cggcaatgct tcgagcacgg tgacctcggc
     4021 accgagccgc cgccacaccg atcccatctc cagcccgatc acgccggcgc cgaccacgcc
     4081 gagccgcccg ggcacttcgc tgatcgccaa cgcgccgtcg ttcgacaaaa taagcttctc
     4141 gtcgaagtct gcaccgggca gcgcacgcgg attcgaaccc gtcgcgacga ctacgtgctt
     4201 ggccgtgagc gtctcgtcgt tgacctggat ccggtagccg ccgtcgcccg ggccggcgaa
     4261 cgagccacgg ccatggaaaa acgcgatctt gttcttcttg aacaggaacg agatgccgtc
     4321 gttgttctgc cgcaccacct tgtccttgcg cgcgagcatc tgcgccacgt ccatcgacag
     4381 gcccttgacc tcgatgccgt gatccttgaa gtggtgcgca gcctgctcgt agttctcgga
     4441 tgactgcagc agcgccttgg agggaatgca gccgacattg gtgcaggtgc cgccgggcgc
     4501 cggcccgccc ttggcgttct tccactcgtc gacacaggcg acgctgaaac cgagttgcgc
     4561 ggcccggatc gcgccgatgt agccgccggg gccggcgccg atgacgacga cgtcaaagct
     4621 cttgctcatc gttgacgttc tccccgccta aacctcgagc agcaggcgcg cgggatcctc
     4681 gagcgcctcc ttcatcgcca ccagcgacag caccgcctcg cgaccatcga tgatccggtg
     4741 gtcgtacgag agcgcgagga aattcaccgg ccggatcacg atctggccgt tctcgacgac
     4801 cgggcggtcc ttggtcgcgt ggatgccgag gatcgcgctt tgcggcgggt tgatgatcgg
     4861 cgtcgacagc atcgagccga acacgccgcc gttggaaatc gagaacgtgc cgccggtgag
     4921 ttcctcgatc gcgagcttgc cttcgccggc gcgcttgccg aagtcggcga tcttcttctc
     4981 gatgtcggcg aacgtcatct ggtcggcgtc gcgcaggatc ggcaccacga gtccgcgcgg
     5041 cgagccgacc gcgatgccga tgtcgaagta gccgtggtag acgatgtcgg ctccgtcgac
     5101 cgacgcattg acgacaggga acctcttcag cgccgcgacg gcggccttga cgaagaacga
     5161 catgaacccg agcttcacgc cgtgctcctt ctcgaacctg tccttgtact tgctgcgcag
     5221 gtccatcagc ggcgccatgt tgacctcgtt gaacgtggtc aggatggccg cggtcgattg
     5281 cgactgcacc agacgttcgg ccacgcgctg ccgcaggcgc gacatcggaa cgcgctgctc
     5341 gggccggtcc gagaccatcc gcatgtcgag cggcgccttg acttcgggca gtgccggccg
     5401 cgccgccggt gtcggtgtcg gtgtcggtgt cggcgcgggc gctgctgctg gcgctgcctt
     5461 gatgccctcc ttggcggcaa tcacgtcgcc cttcaggatg cggccgtcct tgccgctgcc
     5521 cgcgacctgc gcgggcgaca tgccggcatc agcaagcatc ttggcggcgg ctggcatcgc
     5581 gatggcgccg gacgcaggtg cagccgcgcc tatcggcgac ggtgccgccg cgggcaccgg
     5641 tgccggggcg gacgccgccc tcgctccggt cttcg
//
LOCUS       DBNHBY010000676         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1538594, whole genome shotgun sequence.
ACCESSION   DBNHBY010000676 DBNHBY010000000
VERSION     DBNHBY010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1538594"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="AC16HB_16100"
     CDS             complement(<1..1114)
                     /locus_tag="AC16HB_16100"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5608363.1"
                     /translation="MLPPSPSDQPDAEPSIVPDQISAVGTIHLAWQLDALHAFDVADR
                     LVELATRGKLKLGAAPVSTTLTDYAFDPDRLSDAERLHLYARTFGARTSAALEGEINL
                     EFDALWLRFVAAVAESAHEPDPAAASRPPLLARWTVRRAARDLALNLSRHGAERARDE
                     ARTLHRSIERLLHLLQAPEVLKALGAPSMWDAIERVNREHLGSTVDTARGRALAQSGS
                     HIIGWLAAHAADLSGHAASAPSAQADADLIATVERWLAASGTPAAVLAASERPAEAAH
                     ARLDRVPTLSAEVMHALNRIEADARSRPGGVCALFVGRMDAARMLAAQVLASRLAHDL
                     YRVDLAAVAARSIGETDRNLERVLAQARESAAVLLFD"
     gene            1206..1541
                     /locus_tag="AC16HB_16105"
     CDS             1206..1541
                     /locus_tag="AC16HB_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608364.1"
                     /translation="MTKSPPKQAHHVYVIELDPEVLRDRRFRARNPNYIEGMPCVYVG
                     MTGLPVAQRFANHRDGYKSNRYAHRYGVRLLPDLYECFNPMPYHAARQMEQDLADELQ
                     EKGYAVWQG"
BASE COUNT          268 a          632 c          637 g          250 t
ORIGIN      
        1 cgtcgaacag cagcacggcg gccgactcgc gcgcctgcgc cagcacgcgc tcgagattcc
       61 tgtccgtctc gccgatcgac ctcgccgcga ccgccgccag gtcgacgcga tacaggtcgt
      121 gcgcgagccg cgacgccaga acctgcgcgg cgagcatcct ggctgcgtcc atccggccga
      181 cgaagagcgc gcagacgccg cccggccgcg agcgcgcatc ggcctcgatc cggttcaagg
      241 catgcatgac ctcggctgac aaggtcggca cccggtccag ccgcgcgtgc gccgcctcgg
      301 caggtcgctc gctcgccgcg agcacagcgg caggcgtgcc gctcgccgcg agccagcgtt
      361 cgaccgtcgc gatcaggtcg gcgtccgctt gcgccgacgg cgcgctcgcg gcatgcccgc
      421 tcaggtccgc cgcgtgtgcg gcgagccaac cgatgatgtg cgagcccgac tgcgccagcg
      481 cgcgcccgcg cgcggtgtcg accgtgctgc cgaggtgctc gcggttgacg cgctcgatgg
      541 catcccacat cgacggcgca ccgagcgcct tcagaacctc cggcgcctgc agcaggtgca
      601 gcagtcgctc gatgctccgg tgcagcgtcc gcgcttcgtc gcgcgcgcgc tcggcgccgt
      661 ggcgcgacag gttcagcgcc aggtcgcggg cggcgcgccg caccgtccag cgcgccagca
      721 gcggcgggcg cgacgcggcc gccggatcgg gttcgtgcgc gctctccgcc acggcggcga
      781 cgaagcgcag ccacagggcg tcgaattcga ggttgatctc gccctcgagc gcggcgctgg
      841 tccgggcgcc gaatgtgcgg gcgtacagat gcaggcgctc cgcatccgag agccggtcgg
      901 gatcgaaggc gtagtcggtc agggtcgtcg acaccggggc ggcgcccagc ttgagcttgc
      961 cgcgcgtggc gagctccacc aggcggtcgg cgacatcgaa cgcatgcaac gcgtcgagct
     1021 gccacgcgag atggatcgtt ccgactgcgc tgatctggtc cggcacgatc gatggctccg
     1081 cgtccggctg gtcggatggg ctgggcggca gcatggtcgt cggtggcgat tggaactcac
     1141 ataaagtatc gcgtggcgca ggaccccgat acaggcatag cgcaacaacc gggcgagtcc
     1201 gcacagtgac gaagtccccg cccaagcaag cgcaccacgt gtacgtgatc gaactcgatc
     1261 ccgaagtgct gcgcgaccgc cgctttcgcg cgcgcaaccc gaattacatc gagggcatgc
     1321 cgtgcgtgta cgtcggcatg accggcctgc cggtcgcgca gcgttttgcc aatcaccgcg
     1381 acgggtacaa gagcaaccgc tatgcgcaca ggtacggcgt gcggctgctg ccggacctgt
     1441 acgagtgctt caacccgatg ccgtaccacg cggcgcggca gatggagcag gatctcgccg
     1501 acgaattgca ggaaaagggc tacgcggtgt ggcagggttg agtgcggctc gcctgaatcc
     1561 gcgtctgcgc ccggcctggg gcgcgcgtcc gccccaatgc tgcatcgctc gtctgtttgg
     1621 tgtcgttcac agagaatcga ccggatggca ctacacgccg agcgcggcgc gcacgcgctg
     1681 cggcgtgaac ggcacctcgg tcaggcgtgc accggtcgcg tcgaacaccg cgttggccag
     1741 agccgcgggc acgggcgtcg acgcggcttc gccggcgccg agcggcg
//
LOCUS       DBNHBY010000677         1634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1538697, whole genome shotgun sequence.
ACCESSION   DBNHBY010000677 DBNHBY010000000
VERSION     DBNHBY010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1538697"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16HB_16110"
     CDS             complement(<1..326)
                     /locus_tag="AC16HB_16110"
                     /inference="COORDINATES: protein motif:HMM:NF021621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR5608365.1"
                     /translation="MTKLHWIVVANGARAAFYSRDPEDEAFRLNLEQQFAHPEGRSKG
                     EELASDRPGAVRGHGSDSAQYVPHLDPKRNEMEHFGRKLAFELDRLHRAGRLERLTLV
                     ASNPFLG"
     gene            complement(340..1248)
                     /locus_tag="AC16HB_16115"
     CDS             complement(340..1248)
                     /locus_tag="AC16HB_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5608366.1"
                     /translation="MKILIPIDGSRLSQDAAEFVASRSMLVGTSPEVLLVNVQAPLSS
                     QAERIIGRAATRDIYREQAEKVARSAQVLLARSGLAAELRVVVGGAAEAIAREADRSG
                     ADLVVMGSHGRTELKRALLGSVAYGVLARTNVPVLLLRGKAVPRREPLRVAIALDGSR
                     LGLEAVKYALRHPEMFGARPSFALVHVAADFVLPLAGDITGAAATVFTPEQIDQMRNA
                     AFEEVFEPARKLMRRAQAPFTEVRLTGVAGNEIAAYARRHADLTLLGSHGYGAVRAAV
                     LGSVALRVASSGNTPLLIVRPRRTRR"
BASE COUNT          249 a          608 c          530 g          247 t
ORIGIN      
        1 ccgaggaacg ggttcgacgc caccagcgtc agccgctcca ggcgccccgc gcgatgcagc
       61 cggtcgagct cgaaggccag cttccggccg aagtgttcca tctcgttgcg cttcggatcg
      121 agatgcggca cgtactgcgc gctgtcgctg ccgtggccgc gcacggcacc cggccggtcg
      181 ctggcgagct cctcgccttt gctccgacct tccgggtgcg cgaactgctg ctccaggttg
      241 agccggaacg cctcgtcttc gggatcgcgg ctgtagaacg ccgcgcgcgc tccgttggcc
      301 accacgatcc agtgcagttt cgtcatgaca ccactccctt catcgtcgcg tccgacgcgg
      361 ccgcacgatc agcagcggcg tgttgccgct ggaggccacg cgcagcgcga ccgaacccag
      421 caccgccgcc cggaccgcgc cgtatccgtg cgacccgaga agcgtgaggt cggcgtgccg
      481 gcgcgcgtac gcggcgatct cgttgccggc cactcccgtc agcctgacct cggtaaacgg
      541 cgcctgcgcc cgccgcatca gcttgcgcgc gggctcgaac acttcctcga acgctgcgtt
      601 gcgcatctgg tctatctgct cgggcgtgaa gacggtcgca gcggcgccgg tgatgtcgcc
      661 ggcgagcggc agcacgaagt ccgcggccac gtgaaccagc gcaaacgacg gtcgcgcgcc
      721 gaacatctcc ggatggcgca gcgcgtactt gaccgcctcg agcccgagcc ggctgccgtc
      781 gagcgcgatc gccacccgca gcggttcgcg cctcggcacc gccttgccgc gcaacagcag
      841 taccggcaca ttcgtgcgcg ccagcacgcc gtaggcgacc gagccgagca gcgcgcgctt
      901 cagttccgtg cggccgtgcg atcccatcac caccaggtcg gcgccgctgc gatcggcctc
      961 gcgcgcgatc gcctcggcgg cgccgccgac caccactctc aactcggcgg cgagccccga
     1021 gcgcgccagc agcacctgcg cggatcgcgc gaccttctcg gcctgttcac ggtagatatc
     1081 gcgcgtggca gcgcggccga tgatccgctc ggcctgcgac gacagcggcg cttgcacgtt
     1141 gaccagcaga acctcgggcg aggtgccgac cagcatcgag cggctggcga cgaattcggc
     1201 tgcatcctga ctcaaccggc tgccgtcgat gggaatcagg attttcatct gcgctctcga
     1261 atgacacggg ttcgagcgag ggcgcttgtc ctgtggcgca aacttttctt gttcgccctc
     1321 agcaccgaat ttgcgcccga tcggcatcga atccaatcgc ggcgcaataa cgatgttgac
     1381 gctggtcaac cgtatcgggc aaaccccaat tcactcgcgc aacggtgcga tgcgtgtacg
     1441 cgcgtgggcc tgcgggctcg atcgcgccgt cagccccacc gcgtggcgag gaacgcagcc
     1501 gcgaacgatt cacgttccgc ggcaggcagg cgatcgattc cggcagcgcc ggcgcgcccg
     1561 ccgccgccga accgccggca cagcacatcg gcgccgtgcc gcgacgactg cggcgcacgg
     1621 acgctcaccg cgca
//
LOCUS       DBNHBY010000678         2012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1877443, whole genome shotgun sequence.
ACCESSION   DBNHBY010000678 DBNHBY010000000
VERSION     DBNHBY010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1877443"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="AC16HB_16120"
     CDS             complement(<1..539)
                     /locus_tag="AC16HB_16120"
                     /inference="COORDINATES: protein motif:HMM:NF014998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5608367.1"
                     /translation="MKSQPMSPVDAAWFHIDGPVNSAVITSIMTTRRPLDFERVKDVY
                     TQRLAQFGRFRQRVVEKGFPLATPHWEDMPDFQIEQQLHHVALPAPGNRAALTALIED
                     LASTPMDRARPLWDVHVVNGVGNGAALITRIHHCIGDGTANLVVARALFEPAPDAAAD
                     EADEADETAHAPAGRLFAPA"
     gene            complement(698..1123)
                     /locus_tag="AC16HB_16125"
     CDS             complement(698..1123)
                     /locus_tag="AC16HB_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608368.1"
                     /translation="MSDSVRKVHYYSTTVSNRPGEAFRVLSTLVSAGINLLACAGHST
                     GRRSRIDVVPDDERRFSAAAKKARLLFSQKKAGFLIQGDDRAGALADNLKQLADHGIN
                     VASVEGVTAGKGRWGAIVWVDPKDMARAGKVLRAKVEKG"
     gene            complement(1175..1819)
                     /locus_tag="AC16HB_16130"
     CDS             complement(1175..1819)
                     /locus_tag="AC16HB_16130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR5608369.1"
                     /translation="MKHAVVAVLFALACAGAVAQAPHTHQHGFAGAEHWAQVFDDPAR
                     DAWQKPHEVITALALAPDATVADIGAGTGYFSARLAHMLPRGRVFAADIESDMVRYLK
                     ERAQREKLPNLEPVLAAPDDPRLPAKVDRVLVVDTYHHIESREAYFRRLRDSLKPGAE
                     VAIVDFTPDAPMGPPKGVRIPAPRVIDEMQRAGYALAAEHRFLPHQYFLVFRPR"
     gene            1889..>2012
                     /locus_tag="AC16HB_16135"
     CDS             1889..>2012
                     /locus_tag="AC16HB_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR5608370.1"
                     /translation="MTHASHPPNRLARETSPYLLQHAHNPVDWYPWGEEAFAKAR"
BASE COUNT          301 a          711 c          684 g          316 t
ORIGIN      
        1 gccggcgcga acagccgccc ggccggcgca tgcgcggtct catccgcttc gtcggcctcg
       61 tccgccgccg cgtccggcgc gggttcgaac agcgcgcgcg ccacgacgag gttggcggtg
      121 ccgtcgccga tgcagtgatg gatgcgggtg atcagcgccg cgccgttgcc gacaccgttg
      181 accacgtgca cgtcccacag cgggcgcgcg cgatccatcg gcgtgctggc cagatcctcg
      241 atcagcgcgg tcagggcggc gcggttgccg ggcgcgggca gcgcgacgtg gtgcagctgc
      301 tgctcgatct ggaaatcggg catgtcctcc cagtgcggcg tggcgagcgg gaagcctttc
      361 tcgaccacgc gctgacgaaa gcgcccgaac tgtgcgagcc gctgcgtgta gacatccttc
      421 acgcgctcga aatcgagcgg ccggcgcgtc gtcatgatgc tggtgatcac ggccgagttc
      481 accggcccgt cgatgtggaa ccacgccgcg tcgaccggcg acatcggttg cgatttcatc
      541 gaagcttcat cctgcatagg ggcgccggat cgcgcagcgg cgtcctgcga aacttcagga
      601 cgaccgcacg aaagatagcg aggttcccgc gccgccgaat tgacccctca caggttggcg
      661 gatgattccg agcgggccac ccgagcggcc gcgcagctca gcccttttcc actttggcgc
      721 gcagtacctt gccggcgcgg gccatatcct tgggatcgac ccagacgatc gcgccccagc
      781 ggcccttgcc ggcggtcacg ccttccaccg acgcgacgtt gatgccgtga tcggccagct
      841 gcttcaggtt gtccgcgagc gcgccggcgc gatcgtcgcc ctggatcagg aagccggcct
      901 tcttctggct gaacagcagc cgcgccttct tcgccgcggc ggaaaaccgg cgctcgtcgt
      961 ccggcacgac gtcgatccgc gagcggcgcc cggtcgagtg gccggcgcag gcgagcaggt
     1021 tgatgccggc gctgaccagc gtcgacagca cgcggaatgc ctcccccggt cgattggata
     1081 ccgtcgtcga gtagtagtgc accttgcgca ccgagtcgga catcgcggcc tccccttgcg
     1141 tgagcagcgg cggaatcgcc cctagcgtag cgcgtcagcg cggccggaac acgaggaaat
     1201 actggtgcgg caggaacctg tgctcggccg ccagcgcgta gccggcgcgc tgcatctcgt
     1261 cgatcactct cggcgcggga atgcgcacgc ccttgggcgg ccccatgggc gcgtcgggcg
     1321 tgaagtcgac gatcgccact tcggcgccgg gcttgaggct gtcgcgcagg cggcggaaat
     1381 acgcctcgcg cgactcgatg tgatggtacg tgtcgacgac cagcacgcgg tcgaccttgg
     1441 ccggcagacg cggatcgtcg ggcgcggcca gcaccggctc gaggttgggc agcttctcgc
     1501 gctgcgcgcg ctccttcaga taccgcacca tgtcggattc gatgtcggcc gcgaacacgc
     1561 gccctctcgg cagcatgtgc gcgagccgcg ccgagaaata accagtgccg gcgccgatgt
     1621 cggccaccgt cgcgtccggc gccagcgcga gcgcggtgat cacttcgtgc ggcttctgcc
     1681 acgcgtcgcg cgccggatcg tcgaacacct gcgcccagtg ctccgcgccg gcgaagccgt
     1741 gctgatgcgt gtgcggcgcc tgcgcgacgg cgccggcgca tgccagcgcg aacaacaccg
     1801 cgaccactgc gtgtttcatc gttcctcctt ggccaggacg caaactgtag ctcctgcgcg
     1861 ctacgcttgg cctcttccga gcagcacgat gacccacgca tcgcacccac ccaaccggct
     1921 ggcccgcgaa accagtcctt acctgctgca gcatgcgcac aacccggtcg actggtaccc
     1981 gtggggcgag gaggcgtttg ccaaggcgcg cg
//
LOCUS       DBNHBY010000679         9057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1808943, whole genome shotgun sequence.
ACCESSION   DBNHBY010000679 DBNHBY010000000
VERSION     DBNHBY010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1808943"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1091
                     /gene="ypfJ"
                     /locus_tag="AC16HB_16140"
     CDS             213..1091
                     /gene="ypfJ"
                     /locus_tag="AC16HB_16140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5608371.1"
                     /translation="MRTDGRESDNVEDRRGESVGGRGLPIGGRGLGIGTIVIALVASY
                     FLGVDPRTVLSVLGGMDSGAPTQVNVPSGPAPKPQDEIGHFMSVVLADTEDTWGELFK
                     QGGGNYRPPKLVLFRNAYPTACGMGQSAAGPFYCPEDGKVYLDTSFFDLLAQRFGAAG
                     DFAQAYVVAHEIGHHVQNQLGIMEKTAQLRSRMSESQYNQVSVRVELQADCFAGIWAH
                     HANRSRQILEAGDLDEALRAASRIGDDVLQKQAQGYAVPESFTHGSAEQRARWFRAGY
                     ETGSMKACNTFEARQL"
     gene            complement(1171..1578)
                     /locus_tag="AC16HB_16145"
     CDS             complement(1171..1578)
                     /locus_tag="AC16HB_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608372.1"
                     /translation="MADNTSPRDVRMDAGDLWREEIVTDRKIGTIRMMVPIKADGSTD
                     PARATVYTGEAQMMTNMGPLPINFDIEAKSLAEAVSGYGAAAKVAIERTVRELQEMRR
                     EAASGLVLPGQGGFGPAGVPPGLGGGRGKIQMP"
     gene            1646..1807
                     /locus_tag="AC16HB_16150"
     CDS             1646..1807
                     /locus_tag="AC16HB_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608373.1"
                     /translation="MTLFEWLAAVCRELNWHVMRAHDMLDDTRWSDMFERGLTPAQAA
                     ESARRDRLA"
     gene            1821..2705
                     /locus_tag="AC16HB_16155"
     CDS             1821..2705
                     /locus_tag="AC16HB_16155"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR5608374.1"
                     /translation="MPELPDLTVYLEHLDRLLAGESLLRIAVLNPFVLRTVEPKPAAC
                     EGRRFVGTSRIGKRLVLSFDGDLHAVIHLMVAGRLRWLLAGRKPPARITLATFAFEPG
                     TLALTEAGSKRRASLHLAQGAGALAAFDPGGIEPLAMGGDAFRSRLTQANHTLKRVLT
                     DPHLFAGIGNAYSDEILHRARLSPVALTQSLDDEEIGRLQCAVHAVLTEWIERLRAHS
                     RDAFPEDVTAFRPEMAVHGKFGAPCPACGAPVQRIVYADNETNYCARCQTGGRLLADR
                     ALSRLLKASWPKSIDELG"
     gene            complement(2697..3938)
                     /locus_tag="AC16HB_16160"
     CDS             complement(2697..3938)
                     /locus_tag="AC16HB_16160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608375.1"
                     /translation="MRHRLYWRIWFTILASLIAFTLLTGLAWRVLGERTFGPNVRAWA
                     EIAATLLPPASAPVEEQRAALHAWREKLRADLALFGADGAPIGVTASDLQVIPGEEGW
                     LSTRHGPAYALRLPDGRTLVTRRHGARPPPFGLTSTLLVLAVAVGIGAYPVARRITRR
                     LERLRTGVDRLGRGDLAARVQVEGHDEIAQLATRFNAAAERIEQLVQSHRSLLANASH
                     ELRSPLARVRMAAELLGTDARPEVKRELERDIGELDALIDEILLASRLDATDARAEFE
                     RIDLTALVAEECARSGAQLDAGPVAVHGSPRLLHRLVRNLLENARRYGNGAPVEVKLA
                     ADGSARLEVCDRGPGIDADERERIFEPFYRARGAAESAGGVGLGLALVRTIAEQHGGR
                     VDCLPRDGGGSIFRVSLPAST"
     gene            complement(3938..4648)
                     /locus_tag="AC16HB_16165"
     CDS             complement(3938..4648)
                     /locus_tag="AC16HB_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608376.1"
                     /translation="MDNSGVPKLLLIDDDARLAAMVREYLRGHGIDVATAADGARGLA
                     QLGADEFDLVLLDLMLPDADGLDLCRRIRAHGDATAEVPILMLTAKGDPLDRVIGLEM
                     GADDYLPKPFEPRELLARVRALLRRRRASANEHVLRFGRLEIDRGARLVRVGGELRAA
                     TSHQFDLLLALAEHAGRVVSREQLMQWARGSALEAFDRSIDVHIARLRALIEDDPRQP
                     KRIITVRGAGYVFAKVQD"
     gene            complement(4669..5229)
                     /locus_tag="AC16HB_16170"
     CDS             complement(4669..5229)
                     /locus_tag="AC16HB_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5608377.1"
                     /translation="MGNEQMNSGDSNSSRTSRGRRWTLAGLGAASLATIAALGAGWHG
                     HAHARGFGPGGPGFMGRGFGPGMDPEAMGKRLDAMVAFALADTDATPDQKSRISAIVK
                     QAAAELAPLRKTHMEARRKSMALLTAPTIDRAQLEALRVQQMQLGDSVSRRMVQALAD
                     AAEVLTPEQRAKVAQHWQARRGGPAS"
     gene            complement(5348..5971)
                     /locus_tag="AC16HB_16175"
     CDS             complement(5348..5971)
                     /locus_tag="AC16HB_16175"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5608378.1"
                     /translation="MNRERIEAAKTWVLLLALAAATFVAVVFATLWLRAPSARPATSA
                     VAPAPPKADAAGAHSVASDQIAQMIDKLAARLKQQPQDPDGWAMLARSYAVSGRHAEA
                     VPAFRQAVAQRANDPVLLADFADALAVTQGRSFAGEPQTLVDKALQIDPNSVKALSLA
                     GSAAFERKDFAGAVRHWEQVVRVAPPDSPFVPLAQSGMAQARAASGR"
     gene            complement(5974..8469)
                     /locus_tag="AC16HB_16180"
     CDS             complement(5974..8469)
                     /locus_tag="AC16HB_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5608379.1"
                     /translation="MRRSLVAAALISASVGVHAQVVDAIDVRREGADAVIEVRFTTEV
                     QFVRAITTRANDLMFVSYTLVSAFNSQLRASEGQRLGAPQGLPNLRIADEAESGERNR
                     RLVLRFAEPTRARARAGEGNRSIEIVLEGQGGKVEAALGSAFPLAERRFVITLVSSLD
                     PKPQMTQSVPSTLQNYEVFTSQRVVDGRTRHEINLGYFATREQAERALTQLSRFPQAA
                     IVALAPEREAAPVAVAPAPPPPPAPTPGPPPAPPPAAAEAGPLAPAATDAAAAALLAK
                     AKQSYAAKDFAAALQALNELLNLPPSASTREAQELIGMTRLRLGDTARARSEFETYLK
                     LYPQGEGAERVRRELAALPRPAPQPVAQAKPAREKETIVTGSTSLYYFGGNARLRSQD
                     FQDSPVSGLPQVAGDPLLTSDKTRQVLGDVDLGWRQRGPDDDMRFVMRDSYTKDLERP
                     DKSKNRLSALYFDYKSLADGYGFRLGRQSPTGGGVMGRFDGVSANYFIRPKLKFGGVA
                     GVPTDKFFDSNRKFGGLSLDSEGILPNFGVGVYGIQQMIDSEIDRRAVGLEMRYFKGG
                     ASVFSQFDYDTLIKGLNIATVQGTLIMEDSTVFNALYDRRALQMLSLGNALTFTDASG
                     VLFRRIEDRLATTTLEALREQIKRITPDITQAQLGVTKPLTKNWQLAGSVQMTNIGAI
                     PPVPEVIGFETGRPASGNIYTVSGQVIAINLYSERDTQVLSTSRISSPSLNGILVMYN
                     NSSILFESWQFEPSLQYYRDRNTEGGTSKRWTPGLRITYRGFKRWALESNLTYEIGRA
                     TRISSDPNDPTRNSSTQESSTRINYSLGARFEL"
     gene            complement(8498..>9057)
                     /locus_tag="AC16HB_16185"
     CDS             complement(8498..>9057)
                     /locus_tag="AC16HB_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608380.1"
                     /translation="KWTPTKWNHTQVPVTAQCATCHTGGYPPADGKPANHIPYQTVPV
                     TATANCDGCHRGSFTTWANGKLHSYYTVTTGCRSCHTGAYLNARGKPSNHIPEVQLLN
                     GASMECNACHTSTASWSSMTMNHNGSQGSGAGWCKACHQSGTAWLGSMERMSLTHRST
                     SAIDCSQSGCHRPLGTKGTTYINWN"
BASE COUNT         1321 a         3166 c         3119 g         1451 t
ORIGIN      
        1 acgtcatcgc gccgacgccg cgcgtgttcg tgccggcgcc ggtcgtctat cgctcgccct
       61 ggtacggccc acgcgcgtac cggtacaagc actggaagcg ctaccgcgac tggaacgatt
      121 gatttgagct accgcaatcg cgccgcgcac cgctctggag ccggtcgctg cgccgtgttc
      181 caatcggtgt ggtgaccgac cggagcagat gaatgagaac ggacggcaga gagagcgaca
      241 acgtcgagga tcgccgtggc gagagcgtgg gtggccgcgg cctgccgatc ggcggacgcg
      301 ggctgggaat cggcacgatc gtgattgcgc tggttgcgtc gtacttcctc ggcgtcgatc
      361 cgcgcacggt gttgagcgtc ctcggcggga tggattcggg cgcgccgacg caggtcaacg
      421 taccgagcgg accggcgccg aagccccagg acgagatcgg ccacttcatg tcggtcgtgc
      481 tcgccgatac cgaggacacg tggggcgagt tgttcaagca gggcggcggt aactaccggc
      541 cgcccaagct ggtgcttttc aggaatgcgt atccgacggc gtgcggcatg ggccagtcgg
      601 ccgcagggcc gttctactgc ccggaagatg gcaaggtcta tctcgacacg tctttcttcg
      661 acttgctcgc gcagcgcttc ggggccgccg gcgacttcgc gcaggcctac gtggtcgcgc
      721 acgagatcgg ccatcacgtg cagaaccaac tcggcatcat ggaaaagact gcgcaactgc
      781 gcagcaggat gagcgaatca cagtacaacc aggtctcggt gcgcgtcgaa ctgcaggctg
      841 actgctttgc cggcatctgg gcgcatcacg ccaatcgctc gcggcagatt ctcgaagcgg
      901 gcgacctcga cgaagcgctg cgcgccgcca gccgcatcgg cgacgacgtg ctgcagaagc
      961 aggcgcaggg ttacgcggtg cccgaatcgt tcacgcatgg cagcgcggaa cagcgcgcgc
     1021 gctggttccg cgccggctac gagaccggca gcatgaaggc gtgcaacacc ttcgaggcgc
     1081 gccagttgta gtgcatgagg agtgcggcgg cctgcggccg ccatgacggg gctgcgcgcc
     1141 catgaggccg cgcagcgacc accgcgccgt tcagggcatc tggatcttgc cacggccgcc
     1201 accgagaccc ggcggcacac cggctgggcc aaagccgccc tgcccgggca gcaccagccc
     1261 cgacgcggcc tcgcgccgca tctcctgcaa ttcgcgcacg gtgcgctcga tcgcgacctt
     1321 ggccgcggcg ccgtagccgc tcaccgcttc ggccagcgac tttgcttcga tgtcgaagtt
     1381 gatcggcaac gggcccatgt tggtcatcat ctgcgcttcg ccggtgtaga cggtcgcgcg
     1441 cgccgggtcg gtcgagccgt ccgccttgat cggcaccatc atgcggatcg tgccgatctt
     1501 gcgatcggtg acgatctcct cgcgccacag gtcgccggcg tccatgcgca cgtctcgggg
     1561 ggaagtgttg tcggccatcg ggattccttt cactcggcgc gcagcatagc gccgctatga
     1621 tcgttgcgca gaacatgggg gcgacatgac gctattcgag tggctggctg ccgtatgccg
     1681 cgaactgaac tggcacgtga tgcgcgcgca cgacatgctg gacgacacgc gctggtccga
     1741 catgttcgag cgcggcctga cacccgcgca ggcggccgag tcggcgcggc gcgacaggct
     1801 cgcctgagcc gcgctcgccg atgcccgaac tgccggacct caccgtctat ctcgagcacc
     1861 ttgaccgtct tctcgcgggc gaatcactgc tgcggatcgc agtgctcaat ccgttcgtgc
     1921 tgcgaacggt ggaaccgaaa cccgctgcgt gcgagggcag gcgcttcgtc ggcacctcac
     1981 ggatcggcaa gcgactggtg ctgtcgttcg acggcgacct gcatgcggtg atccacctga
     2041 tggtcgccgg caggctgcgg tggctgctgg ccggtcgcaa gccgcccgcg cgcatcacgc
     2101 tcgcgacctt cgcgttcgag ccgggcacgc tggcactcac cgaggccggc agcaagcggc
     2161 gcgcatcgct gcacctcgcg cagggcgccg gcgcgctcgc cgcgttcgat cccggcggca
     2221 tcgagccgct ggcgatgggc ggcgacgctt ttcgatcgcg gctcacgcag gcgaatcaca
     2281 ccttgaagcg cgtactgacc gatccgcatc tgttcgcggg tatcggcaac gcgtattccg
     2341 acgagatcct gcaccgtgcg cggctgtcgc cggtcgcgct gacgcagtcg ctcgacgatg
     2401 aagagatcgg gcgtctgcag tgcgccgtgc acgccgtcct cacggaatgg atcgaacgtc
     2461 tgcgcgcgca cagccgcgat gcctttccgg aagacgtcac ggcattccgg ccggaaatgg
     2521 ccgtgcacgg caagttcggc gcgccctgcc ccgcgtgcgg cgctccggtg cagcggatcg
     2581 tgtacgcgga caacgaaacg aactactgcg cgcgctgcca gaccggaggc aggctcctcg
     2641 ccgaccgcgc gctgtcgcgg ctgctgaagg cgagctggcc gaaatcgatc gacgagctag
     2701 gttgacgcag gcaggctgac acggaagatg ctgccgccgc cgtcgcgagg caggcagtcg
     2761 acgcggccgc cgtgctgttc agcgatcgtg cgtaccaggg ccagcccgag cccgacgccg
     2821 cccgcagact cggccgcgcc gcgcgcgcga tagaacggct cgaagatccg ctcgcgctcg
     2881 tcggcatcga tgcccggccc gcggtcgcac acctcgagcc gcgcactgcc gtcggccgca
     2941 agcttgacct cgaccggcgc cccattgccg tagcggcggg cgttttccag caggttgcgc
     3001 accagccggt gcagcaggcg cggcgagccg tgcaccgcga cgggcccggc gtcgagttgc
     3061 gccccgctgc gcgcgcactc ctcggccacc agcgcggtga gatcgatgcg ctcgaactcg
     3121 gcgcgcgcat cggtcgcgtc gagccggctc gcgagcagga tctcgtcgat cagcgcgtcg
     3181 agttcgccga tgtcgcgctc cagctcccgc ttgacctcgg gccgcgcgtc ggtgccgagc
     3241 aattcggctg ccatccgcac ccgcgccagt ggcgaacgca gttcgtgcga cgcgttggcg
     3301 agcagcgagc ggtgcgactg caccaactgc tcgatgcgct cggccgcggc gttgaagcgc
     3361 gtcgccagtt gcgcgatctc gtcgtgcccc tccacctgca cgcgcgccgc tagatcgccc
     3421 cgcccgagcc gatcgacgcc cgtccgcagc cgctccagcc gccgcgtgat gcggcgcgcg
     3481 accggatagg cgccgatgcc gactgcgacc gcgagcacca gcagcgtcga cgtcagtccg
     3541 aacggcggcg gccgcgcgcc gtgccggcgc gtgaccagcg tgcggccgtc gggcagccgc
     3601 agcgcgtatg ccggcccgtg ccgcgtcgac agccatccct cttcgcccgg gatgacctga
     3661 aggtctgacg cggtgacgcc gatcggcgcg ccgtcggcgc cgaacagtgc gaggtcggcg
     3721 cgcagcttct cgcgccacgc gtgcagcgcg gcgcgctgct cctcgaccgg cgcgctcgcg
     3781 ggcggcagca gcgtggcagc aatctccgcc cacgcgcgca cgttgggccc gaacgtgcgt
     3841 tcgccgagca cgcgccacgc cagccccgtc agcaacgtga acgcgatcag gctggcgagg
     3901 atcgtgaacc agatgcgcca gtagagacgg tggcgcatca gtcctggact ttcgcgaaca
     3961 cgtagcccgc gccgcgcacc gtgatgatgc gcttgggctg gcgcggatcg tcctcgatca
     4021 gcgcgcgcag ccgtgcgatg tgcacatcga tcgaacggtc gaacgcctcg agtgccgagc
     4081 cgcgcgccca ttgcatcaac tgctcgcgcg acacgacgcg tcccgcgtgc tccgccagcg
     4141 cgagcagcag gtcgaactgg tgcgacgtgg cggcgcgcag ttcgccgccg acccgcacga
     4201 gacgcgcacc gcggtcgatc tcgagccggc cgaagcgcag cacgtgctcg ttcgcggacg
     4261 cgcggcgccg gcgcagcagc gcgcgcacgc gcgccagcag ctcgcgcggc tcgaacggct
     4321 tgggcaagta atcatcggcc cccatctcca ggccgatcac gcggtcgagc ggatcgccct
     4381 ttgcggtcag catcaggatc ggcacctcgg cggtcgcgtc tccgtgcgcg cggatgcggc
     4441 gacacaggtc gagaccgtcc gcatcgggca gcatcaggtc gaggagcacg agatcgaact
     4501 catcggcgcc gagctgcgcc agtccgcgcg cgccgtcggc ggcagtggcg acgtcgatgc
     4561 cgtggccgcg cagatactcc cgcaccatcg ccgccaggcg cgcatcgtcg tcgatcagga
     4621 gcagcttcgg cacgccgcta ttgtccaacg gcgtgccgcc tgccgcgatc aactcgccgg
     4681 accgccgcgc cgcgcctgcc agtgttgcgc gaccttggcc cgctgctcgg gcgtgagcac
     4741 ctcggccgcg tcggccagcg cctgcaccat gcggcggctg acggaatcgc cgagctgcat
     4801 ctgctgcacg cgcagcgcct cgagctgggc acggtctatg gtcggcgccg tcagcagcgc
     4861 catcgacttg cggcgcgcct ccatgtgcgt cttgcgcagc ggcgcgagct cggccgcagc
     4921 ctgcttcacg atcgcgctga tgcggctctt ctggtcgggt gtggcgtcgg tgtcggcgag
     4981 cgcgaacgcg accatcgcgt cgagccgctt gcccatcgcc tcggggtcca tcccagggcc
     5041 gaagccgcgc cccatgaagc cgggcccgcc cgggccgaag ccgcgcgcat gcgcgtgccc
     5101 atgccagccc gcgccaagcg ctgcgatcgt ggccagcgac gcggcgccga ggccagccag
     5161 cgtccagcgg cgcccgcggc tcgtgcgcga agagttcgaa tctccggaat tcatctgctc
     5221 gttgcccatg cggtttcctt tcgatcatcg ggcgcgaccg tgcgcccacg gccaagactc
     5281 tgaccgcgcg agatttcgcg cgcatgtgcg ctgtgtaaag ttgtgtaaac gcctgtgcgg
     5341 cggcggacta ccggcccgag gccgcgcgcg cctgcgccat gccgctctgc gccaggggga
     5401 cgaacgggct gtccggcggc gccacgcgca cgacctgctc ccagtggcgt accgcgccgg
     5461 cgaagtcctt gcgctcgaac gcagcgctcc cggccagcga cagtgccttc acgctgttcg
     5521 ggtcgatctg cagcgccttg tccaccaggg tctgcggttc gcccgcgaac gagcggccct
     5581 gcgtgaccgc caacgcgtcg gcgaagtcgg cgagcagcac cgggtcgttc gctcgctgcg
     5641 cgaccgcctg gcggaacgcg ggcacggcct cggcgtgcct gccgctgacc gcatacgagc
     5701 gcgccagcat cgcccagccg tcggggtcct gtggctgctg cttcagccgc gcggcgagct
     5761 tgtcgatcat ctgcgcgatc tgatccgatg ccaccgaatg agcgccggcg gcgtccgcct
     5821 tcggcggtgc gggcgcgacc gcgctcgtcg cgggccgtgc cgacggggcg cgcagccaca
     5881 gcgtggcgaa gacgaccgcg acgaacgtcg ccgccgccag cgcgagcagc agtacccagg
     5941 tcttcgctgc ctcgatgcgc tcccggttca tcgtcacagc tcgaagcgcg cgccgagcga
     6001 gtagttgatc cgcgtcgacg attcctgcgt cgagctgttc cgcgtggggt cgttcggatc
     6061 gctcgagatg cgcgtggcgc ggccgatttc gtacgtcaga ttcgactcca gcgcccagcg
     6121 cttgaagccg cgataggtga tccgcagccc gggcgtccaa cgcttgctgg tgccgccctc
     6181 ggtgttgcgg tcgcggtagt actgcagcga cggctcgaac tgccaggact cgaaaaggat
     6241 ggacgagttg ttgtacatca ccaggatgcc attgaggctc ggactcgaga tccggctcgt
     6301 cgacagcacc tgcgtgtcgc gctccgagta caggttgatc gcgatcacct ggccgctcac
     6361 cgtgtagatg ttgcccgacg ccgggcgacc ggtctcgaag ccgatcacct ccggcaccgg
     6421 cggaatcgcg ccgatgttgg tcatctgcac gctgcccgcg agctgccagt tcttggtgag
     6481 cggcttggtc acgccgagct gcgcctgcgt gatgtcgggg gtgatgcgct tgatctgctc
     6541 gcgcagcgcc tcgagagtcg tggtggccag ccggtcctcg atgcgccgga acaacacgcc
     6601 cgacgcgtcg gtgaaggtga gcgcgttgcc gagcgacagc atctgcagcg ctcggcggtc
     6661 gtacagcgcg ttgaagacgg tgctgtcctc catgatcagc gtgccctgca ccgtggcgat
     6721 gttcaggccc ttgatcagcg tgtcgtagtc gaactgcgag aacaccgacg cgccgccctt
     6781 gaagtagcgc atctccagcc cgaccgcgcg gcggtcgatc tcgctgtcga tcatctgctg
     6841 gatgccgtac acgccgacgc cgaagttggg caggatgccc tcggagtcga gcgacaggcc
     6901 gccgaatttt cggttggagt cgaagaactt gtcggtcggc acgcccgcca cgccgccgaa
     6961 cttcagcttc ggccggatga agtagttggc gctgacgccg tcgaagcgcc ccatcacccc
     7021 gccgcccgtg ggcgactggc ggccgaggcg gaatccatag ccgtcggcaa gggacttgta
     7081 gtcgaagtac agcgccgaga gccggttctt gctcttgtcg ggacgctcga gatccttggt
     7141 gtacgaatcg cgcatcacga aacgcatgtc gtcgtcggga ccgcgctggc gccagcccag
     7201 gtcgacgtcg cccaggacct ggcgcgtctt gtccgaagtc agcaacggat caccggccac
     7261 ctgcggcagg ccgctgaccg gcgagtcctg gaagtcctga ctgcgcaggc gcgcgttgcc
     7321 gccgaagtaa tacagcgacg tcgaaccggt gacgattgtc tccttctcgc gcgcgggctt
     7381 cgcctgcgcg accggttgcg gcgcgggtct gggcagtgcc gccagttcgc gccgcacgcg
     7441 ttcggcaccc tcaccctgcg ggtagagctt caggtacgtt tcgaattcgc tgcgcgcgcg
     7501 cgcagtgtcg cccaaccgca ggcgcgtcat gccgatcagt tcctgcgcct cgcgcgtgct
     7561 ggcgctcggc ggcaggttca gcagttcgtt cagcgcctgc agcgccgctg caaagtcctt
     7621 tgcagcgtag gactgcttgg cctttgccag caatgcggcc gcggccgcgt cggtcgcggc
     7681 gggcgcgagc ggcccggcct cggccgccgc gggcggtggc gcggggggcg gtccgggcgt
     7741 cggcgcgggc ggcggagggg gcgccggtgc caccgcgacc ggcgccgctt cgcgttcggg
     7801 cgcgagcgcg acgatcgccg cctgcggaaa acgcgacaac tgcgtcagcg cgcgctcggc
     7861 ctgctcgcgc gtggcgaagt aaccgaggtt gatctcgtgg cgcgtgcgcc cgtcgaccac
     7921 gcgctgactg gtgaagacct cgtagttctg cagcgtcgac ggcaccgact gcgtcatctg
     7981 cggtttcggg tcgagcgatg acaccagcgt gatcacgaag cgccgttccg cgagcgggaa
     8041 cgcagagccc agcgccgcct cgaccttgcc gccctgccct tcgagaacga tctcgatcga
     8101 ccggttgcct tcgccggcgc gcgcccgcgc gcgcgtcggc tcggcgaagc gcagcaccag
     8161 ccggcggttc cgttcgccgc tttccgcttc gtcggcgatg cgcaggttcg gcagcccctg
     8221 cggcgcgccg aggcgctgtc cctcgctggc gcgcagctgc gagttgaacg cgctgaccag
     8281 cgtgtacgac acgaacatca ggtcattggc gcgcgtggtg atcgcgcgca cgaactggac
     8341 ttcggtcgtg aaccgcacct cgatgactgc gtcggcaccc tcgcgccgca cgtcgatcgc
     8401 gtcgaccacc tgggcatgca cgccgacgga ggcactgatc agcgcggccg cgacgagact
     8461 cctcctcatc ctcgggtaca tattcgcttt cttcttctca attccaattg atgtaggtgg
     8521 ttcccttcgt tccgagcggc cggtggcagc cggactgcga gcagtcgatc gccgacgtgg
     8581 agcggtgcgt gagagacatc cgctccatgc tgccgagcca ggcggtgccg ctctggtgac
     8641 aggccttgca ccagccggcg ccgctgccct ggctgccgtt gtgattcatc gtcatcgacg
     8701 accagctcgc ggtgctggtg tgacaggcgt tgcactccat cgacgccccg ttgagcagtt
     8761 gcacctccgg aatgtgattc gacggtttgc ccctggcgtt gaggtacgcg ccggtgtggc
     8821 agctgcgaca gcccgtggtc accgtgtagt agctgtgcaa cttgccgttc gcccacgtgg
     8881 tgaagctgcc tctgtgacag ccgtcgcagt tcgcggtcgc ggtcaccggt accgtctgat
     8941 acgggatgtg gttggccggc ttgccgtccg ccggcggata gccgccggta tgacacgtcg
     9001 cgcactgagc cgtcaccggc acctgcgtgt gattccactt ggtcggggtc cacttgg
//
LOCUS       DBNHBY010000680         4943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_591969, whole genome shotgun sequence.
ACCESSION   DBNHBY010000680 DBNHBY010000000
VERSION     DBNHBY010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_591969"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16HB_16190"
     CDS             complement(<1..417)
                     /locus_tag="AC16HB_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HIR5608381.1"
                     /translation="MTALKWIVVVVVIASMLLLAAGQLGLLKGRPPANLGVRDGRLQP
                     PSRTPNSVSSQAHLHDAQAYAVDYARIEPLRFSGDGAAALQRLLRIVAAMPGAQVIEA
                     KSDYLYVQFTTRWLRFTDDAEFWLSPSESVIHVRSAS"
     gene            complement(431..1552)
                     /locus_tag="AC16HB_16195"
     CDS             complement(431..1552)
                     /locus_tag="AC16HB_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5608382.1"
                     /translation="MSQQLLLVGGGNAHLAVLDSFARTPLRDTDVTLVSRFDNSIYSG
                     MLPGWIAGHYKLDALTIPLPALAERAGATFFRDRVIAIDLVERVAYTQSGAPLPFDVL
                     SINTGPEIDREAIRGLGDHALTLRPVEKFVEAWQRLEALYTGAAQSATLTIAGGGAAG
                     VEVALAIAHRVGTSPLRLKVQLITGRPGLLPALPASARKHVARLLPKSGVRVIEDEVV
                     AIGADRVQLARAGELPTDVTVVAIGATAARWPRDCGLKCDARGFIAVNSSLQSLSHPF
                     VFAAGDCASMVDHPRARSGVYAIRAGAPLATNLRRAVAGRPLRRHIPPPTALYLLSTG
                     GKNAIGVWGPFTWQSETLWRWKDRIDRAHVARFNPDAPR"
     gene            complement(1703..2338)
                     /locus_tag="AC16HB_16200"
     CDS             complement(1703..2338)
                     /locus_tag="AC16HB_16200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR5608383.1"
                     /translation="MKPPPIAVGVFARPPVAGKAKTRLLPAIGPGGTEKLQAHLIEKT
                     LNVVCHSPRARVTLFIRGDLSHPYITACAHRFNIPTREQVGHNLGARMHAAFVHLLGT
                     YPRALIVGVDNLTLTGNLLLEADEALQKADVVVQPAADGGYTLIGLSRPRAALFKGIP
                     WGTQDVMRLTRERVAQEGLRMAEMQTTWDLDTPTDLGRALREGLLSESVLR"
     gene            complement(2335..3036)
                     /locus_tag="AC16HB_16205"
     CDS             complement(2335..3036)
                     /locus_tag="AC16HB_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR5608384.1"
                     /translation="MRLAIIIPVLDEADTIAAAIERLAELRARGARVIVVDGGSRDDT
                     VRRAAAADRVLCSTRGRSLQMNAGARTPEAVASDVLLFLHVDTVLPPDADRIILGALA
                     NSDRVWGRFDVTIAAEAPRHRAALRVVAWLMNWRSRLTGIATGDQALFIERDSFLALD
                     GFAPVPLMEDIDFCRRARRLSRPIALPERASTSGRRWEKYGVVRTIMLMWRLRAAYFF
                     GADPARLLKRYRDVR"
     gene            complement(3046..3849)
                     /locus_tag="AC16HB_16210"
     CDS             complement(3046..3849)
                     /locus_tag="AC16HB_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR5608385.1"
                     /translation="MQRLLVALLFAPLLALAQGFDHTHAAWTALLARHVKVIANGNAS
                     QVDYRSFAAGRAALDAYVKTLSDVPAAEFAKWTRAQQFAFLANAYNALTIEKVLTRYP
                     NLKSIRDFGRVIGNPWKDEFFTLLGAKHSLDWIEHETLRAPGIYDDPRVHVAVVCASV
                     GCPMLSNEAFTAAKLDTQLEDLFRRFMSDRSRNRYDPQTKAVELSSIFDWYGKDFEKG
                     HRGFSSVQDVVVKYADQLADSPADRQLLRTGTVPIRFLDYDWSLNDIRR"
     gene            complement(3857..>4943)
                     /locus_tag="AC16HB_16215"
     CDS             complement(3857..>4943)
                     /locus_tag="AC16HB_16215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR5608386.1"
                     /translation="VPPIPGIETVGYLTSDTVWNLRQLPKRLVVLGGGPIGSELTQAF
                     ARFGSAVTQVEMLPRIMIREDPEVSELVKKQFESEGIRVLTGHKAKQFVVENGEKIAI
                     VEHKGQDIRIPFDALLVAVGRVANTQGYGLEELGIGTTKARTVETDDYLQTIYPNIFA
                     CGDVAGPYQFTHTAAHQAWYAAVNALFGTFRRFKADYRVIPWATFTEPEVARVGLNET
                     EAKERNIAHVVTVYGIDDLDRAIADGEAHGFVKVLTKPGTDQILGVTIAGEHAGDLLA
                     EYVLAMKYNLGLAKILGTIHTYPTLAEANKYAAGNWKRSTVTQGQWAFLTAFQAWRRG
                     AFGLGTVLGKVGPLLSDKRKAYAAEAH"
BASE COUNT          781 a         1666 c         1708 g          788 t
ORIGIN      
        1 cgatgccgag cggacatgga tcacggactc cgacggcgac agccagaact cggcgtcgtc
       61 ggtgaacctg agccagcgcg tggtgaactg gacataaagg tagtcggact tcgcttcgat
      121 cacttgcgcg ccgggcatgg cggcgacgat ccgcagcagc cgctgcagcg ccgccgctcc
      181 atcaccgctg aagcgcagcg gctcgatgcg cgcgtagtcg acggcgtacg cctgcgcatc
      241 gtgcagatga gcctggctgc tgacgctgtt gggcgtgcgc gagggcggct gcaggcggcc
      301 gtcgcgcacg ccgaggtttg caggcggccg gcccttcaac aggccgagtt ggcccgcggc
      361 cagcagcagc atcgacgcga tcaccactac gaccacgatc cacttcagcg ctgtcatcgg
      421 cggtcctttc tcagcgcggc gcgtcgggat tgaagcgcgc gacgtgcgcg cggtcaatgc
      481 ggtccttcca gcgccacagt gtctcgctct gccaggtgaa cgggccccag acgccgatcg
      541 cgttcttgcc acccgtcgac agcaggtaca gcgcggtcgg cggcgggata tgtcgccgca
      601 gcggccggcc cgcgacggcg cgccgcaggt tggtcgccag cggcgcgccg gcgcgaatcg
      661 catagacacc gctcctggcg cgcgggtggt cgaccatcga cgcgcagtcg ccggcggcga
      721 acacgaacgg gtgcgacagc gattgcagcg acgaattcac cgcgatgaag ccgcgcgcgt
      781 cgcatttgag cccgcagtcg cgcggccagc gcgcggcggt ggcgccgatg gcgacgaccg
      841 tcacgtcggt cggcaactcg ccggcgcggg ccagttgcac gcggtcggcg ccgatcgcga
      901 cgacctcgtc ctcgatcacg cgcacgccag acttcggcaa cagccgcgcg acgtgcttgc
      961 gcgcgctggc gggcagcgcc ggcagcaggc cgggccggcc ggtgatcagt tgcaccttca
     1021 ggcgcagcgg cgacgtgccg acgcgatggg cgatcgccag cgcgacttcg accccggcgg
     1081 cgccgccgcc agcgatcgtc agcgtggcgg actgcgcggc gccggtgtag agcgcttcga
     1141 ggcgctgcca tgcctcgacg aacttctcca ccgggcgcag ggtcagcgcg tggtccccca
     1201 ggccgcggat cgcctcgcga tcgatctcgg gtccggtgtt gatcgacagc acgtcgaatg
     1261 gcaatggcgc gcccgactgc gtataggcga cgcgttcgac caggtcgatc gcgatcacgc
     1321 gatcacggaa gaacgtcgct ccggcgcgtt cggccagcgc cggcagcggg atcgtcagcg
     1381 cgtcgagctt gtagtgcccg gcgatccagc cgggcagcat gccggagtag atcgagttgt
     1441 cgaagcgcga taccagcgtg acgtcggtgt cgcgcagcgg ggtgcgcgcg aaggaatcga
     1501 gcaccgcgag gtgcgcgttg ccgccgccga ccagcagcag ttgctggctc atcggcagat
     1561 cggcgccaca acggcggcgt ggctggacaa cggcgctccc atgtcggggg cggagcatac
     1621 gacgccgcgc gcgcaggcgg gcggggaatg ccgcgcggcg gtgcttgcgt cgatcgcggt
     1681 gacgtgcggc cggagccggc ggctaccgaa ggaccgattc cgacagcagg ccttcgcgta
     1741 acgcgcggcc gaggtcggtc ggggtgtcga gatcccaggt cgtctgcatt tcggccatgc
     1801 gcaggccttc ctgcgcgacg cgctcgcggg tgagccgcat cacgtcctgc gtgccccacg
     1861 gaatgccctt gaacagcgcg gcgcgcggcc gcgacaggcc gatcagcgtg tagccgccgt
     1921 cggcggcagg ctgcacgacg acatcggcct tttgcagcgc ctcgtcggcc tcgagcagca
     1981 ggttgcccgt cagcgtcaag ttgtcgacgc cgacgatcag cgcgcgcgga tacgtgccga
     2041 gcaggtgcac gaatgccgcg tgcatgcgcg cgccgaggtt gtgaccgacc tgctcgcgcg
     2101 tcggaatgtt gaagcggtgc gcgcacgcgg tgatgtacgg atgcgagagg tcgccgcgaa
     2161 tgaacagcgt gacgcgcgcg cgcgggctgt ggcagacgac gttcagcgtc ttttcgatca
     2221 ggtgcgcctg cagcttctcg gtgccgccgg gtcctattgc cggcaacagc cgcgtcttcg
     2281 ccttgccggc gaccgggggc cgcgcaaata cgccaaccgc tatcggaggt ggtttcatcg
     2341 aacgtcgcga tatcgcttca gaagccgcgc cggatccgcg ccgaaaaagt atgcagcgcg
     2401 cagacgccac atcagcatta tcgttcgaac tacaccgtac ttttcccacc gtcggccgga
     2461 cgtactagcc cgctcgggta gagcgatcgg acgagacagt cggcgcgcgc ggcgacagaa
     2521 gtcgatgtcc tccatcagcg gaaccggcgc gaagccgtcg agtgcgagaa agctgtcgcg
     2581 ctcgatgaac agcgcctggt cgccggtggc gatgccggtc agccgcgagc gccagttcat
     2641 cagccacgca accacgcgca gcgcagcccg gtgccggggg gcctcggccg cgatcgtcac
     2701 gtcgaaacgc ccccacacgc ggtccgaatt cgcgagcgcg ccgaggatga tccggtcggc
     2761 gtccggcgga agcaccgtgt cgacgtgcag gaagagcagc acgtcgctcg ccaccgcctc
     2821 gggcgtgcgc gcgcctgcgt tcatctgcag gctgcgcccg cgggtggaac agagcacgcg
     2881 gtcggcggcc gcggcgcgcc gcacggtgtc gtcgcggctg ccgccatcga cgacgatcac
     2941 gcgcgcgccg cgcgcgcgca actcggccag ccgctcgatc gcggcggcaa tggtatccgc
     3001 ctcgtcgagg acggggatga tgatcgccag gcgcacgccc gaatgctatc tgcgaatgtc
     3061 gttcagcgac cagtcgtagt cgaggaacct gatcggcacc gttccggtgc gcagcagctg
     3121 gcggtccgca gggctgtcgg cgagctggtc cgcatacttc acgaccacgt cctgcacgct
     3181 ggagaaaccc cggtggcctt tctcgaagtc cttgccgtac cagtcgaaga tcgacgacag
     3241 ctcgaccgcc ttcgtctggg ggtcgtagcg atttcgtgag cgatccgaca tgaagcggcg
     3301 gaacagatcc tcgagctgcg tgtcgagctt cgcggcggtg aacgcctcgt tcgacagcat
     3361 cgggcagccg accgacgcgc agacgaccgc gacgtgcacg cgcggatcgt cgtatatgcc
     3421 cggtgcgcgc agcgtctcgt gctcgatcca gtcgagactg tgcttcgcac ccagcagcgt
     3481 gaagaactcg tccttccacg gattgccgat cacccggccg aagtcgcgga tcgacttcag
     3541 gttcggatag cgggtcagga ccttttcgat cgtcagcgcg ttgtatgcgt tggcgaggaa
     3601 ggcgaactgc tgcgccctgg tccacttcgc gaactcggcc gccggcacat ccgacagcgt
     3661 cttgacgtac gcgtccagcg ccgcgcggcc ggcggcgaag ctcctgtaat cgacctgcga
     3721 tgcgttgccg ttggcgatga ccttcacgtg cctggcgagc agtgcggtcc acgcggcgtg
     3781 ggtgtggtcg aagccctgcg caagtgcgag cagcggcgcg aacagcagcg cgaccagcag
     3841 ccgctgcatc gcgaggtcaa tgcgcttcgg ccgcatacgc cttgcgcttg tcgctcaaca
     3901 gcggtccgac ctttcccagc accgtgccca gcccgaacgc gccacgccgc cacgcctgga
     3961 acgcggtcag gaatgcccac tggccctgcg tgacggtcga gcgcttccag ttgccggcgg
     4021 cgtacttgtt ggcctccgcc agtgtcggat aggtgtggat cgtgccgagg atcttggcga
     4081 gccccaggtt gtatttcatc gccagcacgt attcggcgag cagatcgccc gcatgctcgc
     4141 cggcgatcgt cacgccgagg atctggtcgg tgcccggctt ggtcaggacc ttgacgaaac
     4201 catgcgcctc gccgtcggcg atcgcgcggt cgaggtcgtc gatcccatag accgtcacga
     4261 catgggcgat gttcctctct ttcgcctcgg tttcgttcag gccgacgcgc gcgacttccg
     4321 gctccgtgaa cgtcgcccac gggatcacgc ggtaatcggc cttgaaacgc ctgaacgtgc
     4381 cgaacagcgc gttgaccgcc gcgtaccagg cctgatgcgc ggccgtgtgt gtgaactggt
     4441 acgggcctgc gacgtcgccg caggcgaaga tgttcggata gatggtctgc agataatcgt
     4501 cggtctcgac ggtgcgtgcc ttcgtcgtgc cgatgcccag ttcctcgagc ccgtagccct
     4561 gcgtgttggc cacgcgaccg accgcgacca gcagcgcatc gaacgggatg cggatgtcct
     4621 gtcccttgtg ctcgacgatg gcgatcttct cgccattttc gacgacgaac tgcttggcct
     4681 tgtgcccggt cagcacgcgg atgccctcgg actcgaactg cttcttcacc agttcggaga
     4741 cctcggggtc ctcgcggatc atgatccgcg gcagcatctc gacctgcgtc accgcgctgc
     4801 caaaacgcgc gaacgcctgc gtgagttcgc tgccgatcgg accgccgccg agcacgacga
     4861 ggcgcttggg aagttggcgc aggttccaca cggtgtccga cgtcaggtag ccaaccgtct
     4921 cgatgcccgg gatcggcggc acg
//
LOCUS       DBNHBY010000681         6889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1877552, whole genome shotgun sequence.
ACCESSION   DBNHBY010000681 DBNHBY010000000
VERSION     DBNHBY010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1877552"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="AC16HB_16220"
     CDS             complement(<1..792)
                     /locus_tag="AC16HB_16220"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HIR5608387.1"
                     /translation="MRAVYYERTGAARDVLHLGELPTPAPGEGEVRIRLATSGVNPSD
                     VKSRAGLRSSALPFPRVVPHSDGAGLIDVVGEGVPASRVDERVWTWNAAWQRAFGTAA
                     QYVTLPAAQAVALPAGVAFDAGACLGIPALTALHAVRTDGGVAGKTVLVAGGAGAVGH
                     YAVQMARLLGARRVIATVSNAQKARLAQHAGADLVLDYRRDDVVARVRDVTGEAGVDR
                     VIEVDFAANVALDRAVLKPEGDLVVYGSGKPEIAVPFFSSIVKNVR"
     gene            1038..1241
                     /locus_tag="AC16HB_16225"
     CDS             1038..1241
                     /locus_tag="AC16HB_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5608388.1"
                     /translation="MAQGTVKWFNESKGYGFITPEDGGKDLFAHYSSIQGNGFKSLQE
                     GQRVEFVVAPGAKGPQATQIRPL"
     gene            1326..1490
                     /locus_tag="AC16HB_16230"
     CDS             1326..1490
                     /locus_tag="AC16HB_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608389.1"
                     /translation="MAKPNYSFEKRQRELAKKKKQEAKRERKAQRTEPDKSAPPAEET
                     NTTPAPGETQ"
     gene            complement(1491..2129)
                     /locus_tag="AC16HB_16235"
     CDS             complement(1491..2129)
                     /locus_tag="AC16HB_16235"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR5608390.1"
                     /translation="MNANRDGLVTGDDNLQRCFWCVSATDYIDYHDREWGFPVDDDRR
                     LFEKVCLEGFQAGLSWLTILRKRTAFRRAFADFDYERLARFGAGDVTRLLGDAGIVRH
                     RGKIESTLNNARRACDLADEFGSLAAYFWSFEPDPATRPARLDWAALRKMGTSPQAMA
                     LSHDLKRRGWTFVGPTTVYAFMQAMGLVNDHIEGCAVRARALAARIALDRLS"
     gene            complement(2126..2896)
                     /locus_tag="AC16HB_16240"
     CDS             complement(2126..2896)
                     /locus_tag="AC16HB_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608391.1"
                     /translation="MKRLVLLPPLLIIAAVIALALHGPIAQWADYHRFADARPLFGMP
                     NALDVLSNAAFAAVALCGWVTLAPRRQSPGLAHGWPGWTLFLAAMVLTAIGSTWYHLA
                     PDDFRLLLDRLPIALACAALLAAVFSDTHRFPHGLALTAGLALFGVGTVLWWYATELR
                     GAGDLRPYALLQVAPLVLVPLWQAIDRAPRGERIAFAAAIGCFVLAKLAEVYDREVLE
                     ALGLLSGHTLKHLLAAAAGALVVAGARRRADALRRSEP"
     gene            3090..4895
                     /locus_tag="AC16HB_16245"
     CDS             3090..4895
                     /locus_tag="AC16HB_16245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608392.1"
                     /translation="MANLSRDQPSQPATSGLVIAVVSFGVLIVLSMWVGVHYVTARER
                     EHRITEARQENANLARVLEEHTIRTLAYIDELTLLIRERFERDGTRLDLPALFGALNV
                     PKTLVRNAVISDETGYIVLGSHGAPHTYLGDREHVRVHEARDSGGIFISKPVLARVNG
                     NWSIVVTRRANKPDGSLLGVIGIAIDPLYFSDFYKDVDLGRDSVVTLVGTDGVIRARL
                     PPGQGHGMGTDIRNSMLFQQLKAAPAGTYVTHAAVDGIERIFAYRQASDYPLIVVVGT
                     SLQEALAPVHGQQRTYWLLAAVQTILLAALSAALVYLVRRRDRAEAAARRYLEELHRR
                     ARQLETARAEAEAGSRAKSEFLATMSHEVRTPMNGVMGMLELLQRSRLDAQQQRFAKV
                     AYDSAAGLLQVLNDVLDFSRMEAGGMTLENAPCNPRDPVREVAALYGESARAKGLELR
                     VQIGHDVPEAVLGDAGRLRQVLTNLVSNALKFTATGAIDVRLLRVPHDPPDAGACRLR
                     FEVGDTGIGLSDDEQRRVFAPFAQADASTTRRFGGTGLGLAICQHLVKLMEGSIGVHS
                     APGQGATFWFEVTLQNAGTREQQASARVARPAPAG"
     gene            4912..5499
                     /locus_tag="AC16HB_16250"
     CDS             4912..5499
                     /locus_tag="AC16HB_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR5608393.1"
                     /translation="MLPEPASARPASTGDLFITFTLLALQGFGGVLAIAQRVLCEQKR
                     WLTPAQFVEVLALAQVLPGPNVCNVALMVGDRFFGWRGAFAALAGMMVLPLVIVLAVT
                     ALYTHYAVHPAVAGALRGMGAIAAGLIVGTALRLAAALRTNAMGVPACIGFAAAAFVA
                     VALLRLPLVWVLLGLGTIAFGVAWRRLDPAAAGSK"
     gene            5496..6032
                     /locus_tag="AC16HB_16255"
     CDS             5496..6032
                     /locus_tag="AC16HB_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR5608394.1"
                     /translation="MTGALPLADLAALFGHFLLLSLLAIGGAIVVAPDMHRVMVTQMG
                     LLTDAQFNASVAIAQASPGPNVLFVAVMGYQAAGLVGAAVTLLGILLPSTTLAFVAAR
                     WGHARREQRAVRAFNAGMAPIVIALLVATGWILSAQTPGWKHLLLTIPAAVLVWRTRI
                     HLLWLIAAGGIIGALGWV"
     gene            complement(6007..>6889)
                     /locus_tag="AC16HB_16260"
     CDS             complement(6007..>6889)
                     /locus_tag="AC16HB_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5608395.1"
                     /translation="AAAVTRLAPLLAAAAFGVQVGAAIVATRFVVGETTPAALALLRY
                     TIALACLAPFVPSIRFARRDLAPIALLGIGQFGVLIALMNYGLQFIASARAGLLFATM
                     PLATLVLGAVLGRERLSARVLFGVLLTIVGVACVLGEKLLVGASRGEWIGALAVLGSA
                     GVGALCSVLYRPYLERYPTAAVGASAMAASVVALAVLAGAEGFFARLPRFSAAGWCAV
                     LFIGASSGIGYYLWLWALRRAEPTRVTIFLSLSPVTAALLGALLLGEVLTSWLMLGLA
                     LIVAGLWSGVRPNPARR"
BASE COUNT         1130 a         2391 c         2384 g          984 t
ORIGIN      
        1 gcggacgttc ttgacgatcg acgagaagaa cggcactgcg atttccggct tgccgctgcc
       61 gtagacgacg aggtcgccct cgggcttcag cacggcgcgg tcgagcgcga cgttggcggc
      121 gaagtcgact tcgatcactc gatccacgcc cgcctcgccg gtgacgtcgc gcacgcgcgc
      181 gaccacgtcg tcgcgccggt agtcgagcac gaggtcggcg ccggcatgct gcgcgagccg
      241 tgctttctgt gcattgctga ctgtcgcgat cacgcgccgt gcgccgagca gcctggccat
      301 ctgcaccgcg tagtgtccga ccgccccagc gccgccggcg accagcaccg tcttgccggc
      361 gacgccgccg tccgtccgca ccgcgtgcag cgcggtcagc gcgggaatac ccaggcacgc
      421 gccggcatcg aatgccacgc cggccggcaa cgcgaccgcc tgcgccgcgg gcagcgtcac
      481 gtactgcgca gcggtgccga acgcgcgctg ccaggccgcg ttccaggtcc acactcgctc
      541 gtcgacgcgt gaggccggca cgccctcgcc cacgacgtcg atcaagccgg cgccgtcgct
      601 gtgcggcacg acacgcggaa acggcaatgc gctcgagcgc aggccggcgc gcgatttcac
      661 gtccgacgga ttcacgcccg acgttgcgag cctgatccgc acttcgccct cgcccggagc
      721 gggggtcggc agttcgccca gatgcagcac gtcgcgcgca gcgcccgtgc gctcgtagta
      781 gacagcccgc atcgaatcgt ctccgcgtgt gggggaggcg cgattctcgc acccgggcgg
      841 cgcgcgattg gacaagtgaa ccgataccgc gtaggctccc gcccgctctc gtttaagtgt
      901 cgtcagtcct tcggtaccca gcgtcggccc gtttcgcggc cgcacctcag ttccccggtt
      961 tacgtacttg atcgtttgca cgccggccgc cgtcgcggcg ggcgatgttc ttcaactcaa
     1021 ttctcaagga agtagacatg gcacaaggta cggtcaagtg gttcaacgag tccaagggct
     1081 acggtttcat cacgcccgag gacggcggca aggatctgtt cgcgcactac tcgtcgatcc
     1141 agggcaacgg cttcaagtcg ctgcaggaag gccagcgcgt cgagttcgtc gtcgcgccgg
     1201 gcgccaaggg cccgcaggcg acacagattc gcccgctctg aggttcgatc gcctgacctg
     1261 aagaccgcca cctcgcgtgg cggttttttc ttgcaaacta cgggttggat cactcaggag
     1321 tcgccatggc gaagcccaac tactctttcg aaaagcgtca acgcgaactt gcgaagaaga
     1381 agaagcagga ggccaagcgc gagcgcaagg cgcagcggac cgagccggac aagtccgcgc
     1441 cgccggcgga ggagacgaac accacgcccg cgccaggcga aacgcagtag tcacgacaag
     1501 cgatcgaggg cgatacgcgc cgcgagcgca cgcgcccgga ccgcacatcc ttcgatgtga
     1561 tcgttgacga ggcccatcgc ctgcatgaac gcgtagacgg tggtgggccc gacgaatgtc
     1621 cagccgcggc gtttcaggtc gtggctcaat gccatcgcct gcggcgacgt gcccatcttc
     1681 cgcagggcgg cccagtcgag gcgcgccggc cgggtcgctg gatcgggctc gaaactccag
     1741 aagtaggcgg cgagcgagcc gaattcatcc gccaggtcgc aggcgcggcg cgcattgttg
     1801 agcgtcgatt cgatcttgcc gcgatgacgc acgatgcccg catcgcccag cagccgagtc
     1861 acatcgcccg caccgaagcg cgccaagcgc tcgtagtcga agtcggcgaa cgcgcgccgg
     1921 aaggcagtcc gcttgcgcag gatggtgagc cagctcaggc ccgcctggaa tccttcgaga
     1981 cagaccttct cgaacaggcg ccggtcatcg tcgaccggaa aaccccattc gcggtcgtga
     2041 tagtcgatgt agtcggtcgc ggatacgcac cagaagcagc gctgcaggtt atcgtcgccc
     2101 gtcaccagtc cgtcgcggtt cgcgttcacg gttcgctccg tcgcaatgcg tccgcccgac
     2161 ggcgcgcgcc ggccaccacc agcgcccccg cggcggcggc cagcaggtgc ttcagcgtat
     2221 ggccgctgag cagcccgagc gcctcgagca cctcgcgatc gtagacctcg gccagcttgg
     2281 cgagcacgaa gcagccgatc gcggccgcga acgcgatgcg ctcgccgcgc ggcgcacgat
     2341 caattgcctg ccatagcggc accagcacca gcggcgcgac ctgcaggagc gcatacgggc
     2401 gcagatcgcc ggcaccgcgc agctcggtcg cgtaccacca cagcacggtg ccgacgccga
     2461 acagcgcgag ccccgccgtc agcgcgaggc catgtgggaa gcggtgcgtg tcggagaaga
     2521 cggcggcgag cagcgcagca catgcgagtg cgatcggcag tcggtcgagc agcagccgga
     2581 agtcgtccgg cgcgaggtgg taccaggtcg aaccgatcgc agtcagcacc atcgcagcga
     2641 gaaacagcgt ccagccgggc cagccgtgtg caaggcccgg cgactgccgt cgaggcgcaa
     2701 gcgtgaccca gccgcagagc gcgacggccg caaatgcagc gttcgacagg acgtccagcg
     2761 cgttcggcat gccgaacagc gggcgtgcat cggcgaagcg gtggtaatcg gcccactgcg
     2821 cgatcggacc gtgcagtgcg agcgcgatca cggcggcaat gatcaggagc ggcggcagca
     2881 ggaccaggcg tttcatgtcg gcctcgcgat gatgatatgg cgccgacgcc gcagcgcggg
     2941 acaggtcccg ccagcggaca tgccgccctt gccgcgcggc ggtgtcgccg cgcgttgcag
     3001 cgtcaaatac aacacgcaag cgccgcgatc ggtggcaaga tcgcgccggc cgcacggcga
     3061 tccaaccgac gggtgcggag gagtgcgcga tggccaatct gtcacgcgat caaccgtcgc
     3121 agcccgcaac cagcggactg gtgattgcgg tcgtgtcgtt cggcgtgctg atcgtcctgt
     3181 cgatgtgggt cggcgtgcat tacgtcaccg cgcgcgagcg cgagcaccgg atcaccgaag
     3241 ccaggcagga gaacgcgaac ctcgcgcgcg tcctcgaaga acatacgatc cgcacgctcg
     3301 cgtacatcga tgaactgacg ctgctgatcc gcgaacggtt cgagcgcgac ggcacccgcc
     3361 tcgacctgcc ggcgctgttc ggcgcactca acgtgcccaa gacgctggtg cgcaacgcgg
     3421 tgatcagcga cgagaccggc tacatcgtgc tcggctcgca tggcgcgccg cacacctacc
     3481 tcggcgaccg cgagcacgtg cgcgtgcacg aggcgcgcga cagcggcgga atcttcatca
     3541 gcaagccagt gctggcgcgc gtgaacggca actggtcgat cgtcgtcacg cgtcgcgcca
     3601 acaagccgga tggttcgttg ctgggcgtga tcggcatcgc gatcgatccg ttgtacttct
     3661 cggacttcta caaggacgtc gacctcggac gcgacagcgt cgtcacgctg gtcggcaccg
     3721 acggcgtcat ccgcgcgcgg ctgccgccgg gacaggggca cggcatgggc accgacatcc
     3781 gcaattcgat gctgtttcag cagctcaagg ccgcgcccgc cggaacgtac gtcacccatg
     3841 ccgccgtcga cgggatcgag cggatcttcg cgtatcgcca ggcaagcgat tacccgctga
     3901 tcgtcgtcgt cggcacgtcg ctgcaggagg cgctggcccc agtgcacggg cagcagcgca
     3961 cctactggct gctggcagcc gtgcagacga tcctgctggc agcgctttcc gcggcactgg
     4021 tttacctcgt gcggcgacgc gatcgcgccg aggccgccgc gcgccgctac ctggaggaac
     4081 tgcatcgcag ggcccggcaa ctcgaaaccg cgcgcgccga ggcggaggcg ggttcgcgcg
     4141 ccaaatcgga gttcctggcc acgatgagcc acgaagtccg cacgccgatg aacggcgtga
     4201 tgggcatgct cgaactgctg cagcgctcgc ggctggacgc gcagcagcag cggttcgcca
     4261 aggtcgcata cgactcggcc gcgggactgc tgcaagtgct gaacgacgtg ctcgacttct
     4321 cgcgcatgga agcgggcggc atgacgctgg aaaacgcgcc ttgcaatccg cgcgatccgg
     4381 tacgcgaagt tgcggcactc tacggcgaat cggcgcgtgc caaaggcctc gaactgcggg
     4441 tgcagatcgg ccatgacgtg cccgaggctg tgctcggcga cgccgggcgg ctgcgccagg
     4501 tgctgacgaa cctcgtcagc aacgcgctga agttcaccgc gaccggcgcg atcgacgtgc
     4561 gcctgctgcg cgtgccgcac gatccgcccg acgccggtgc ttgccgcctg cgcttcgagg
     4621 tcggcgatac cggcatcggc ctgagcgacg acgaacagcg gcgcgtgttc gcacccttcg
     4681 cgcaggccga cgcatcgacg acgcgccgct tcggcggcac cgggctgggt ctggcgatct
     4741 gccagcacct ggtcaaactg atggagggtt cgatcggcgt gcacagtgcg ccgggccagg
     4801 gcgcgacatt ctggttcgag gtcacgctgc agaacgccgg tactcgcgag cagcaagcgt
     4861 ccgcgcgcgt ggcgcggccg gcaccggccg gctgaccggt agcattcacg cgtgctgccg
     4921 gaacccgcat ccgcgcgtcc cgcttcgacg ggcgatctct tcatcacctt cacactgctc
     4981 gcgctgcagg ggttcggcgg cgtgctggcg atcgcccagc gtgtgctgtg cgagcagaag
     5041 cgttggctca cgccggcgca gttcgtcgag gtgctggcgc tggcgcaggt gctgccggga
     5101 ccgaacgtgt gcaatgtcgc gctgatggtc ggcgaccgat tcttcggctg gcgcggcgcc
     5161 ttcgctgcgc tcgccggaat gatggtgctg ccgctcgtga tcgtgctcgc ggtcaccgcg
     5221 ctgtacacgc actacgcggt gcacccggcg gtcgcaggcg cgctgcgcgg catgggcgca
     5281 atcgccgccg ggctgatcgt cggcactgcg ctgcggctgg cggcggcgct gcgcaccaat
     5341 gcgatgggtg tgccggcgtg catcggcttc gccgcggcgg cattcgtcgc cgtcgcgctg
     5401 ctgcggctgc cgctggtgtg ggtgctgctc ggactgggca cgatcgcgtt tggcgtcgcg
     5461 tggcggcgtc tcgacccggc agcggcgggc agcaaatgac gggcgcgctc ccgctggccg
     5521 acctggcagc gctgttcggc cacttcctgc tgctgtcgct gttggcgatc ggcggtgcga
     5581 tcgtcgtcgc gcccgacatg catcgcgtga tggtcacgca aatggggctg ctgaccgatg
     5641 cgcagttcaa cgcgtcggtc gcgatcgcgc aggcttcgcc cgggccgaac gtgctcttcg
     5701 tcgcggtgat ggggtatcag gcggcgggac tggtcggcgc ggcggtcacg ctgctgggga
     5761 tactgctgcc gtcgaccacg ctggcgttcg tcgcggcgcg ctggggccac gcgcgccgcg
     5821 agcagcgcgc cgtgcgcgcc ttcaacgccg gcatggcgcc gatcgtgatc gcgttgctcg
     5881 tggccaccgg ctggatcctg agcgcgcaga cgccgggctg gaagcacctg ctgctgacga
     5941 ttcccgctgc ggttctggtg tggcgcacgc gcatccacct gctgtggctg atcgccgccg
     6001 ggggcatcat cggcgcgctg ggttgggtct gacgcccgac cacaggccgg ccacgatcag
     6061 cgccagcccg agcatcagcc atgacgtcaa gacttcgccg agcagcagcg cgccgagcag
     6121 cgccgcggtc accggactga gcgacaggaa gatcgtcacg cgcgtgggct ccgcgcggcg
     6181 cagcgcccac aaccacaggt agtagccgat cccgctgctc gcaccgatga acagcaccgc
     6241 gcaccacccc gcagcggaga aacgcggcag gcgcgcgaag aatccctcgg cgccggccag
     6301 aaccgccagc gcgacgaccg aagcggccat cgccgaggca ccgaccgccg cggtcgggta
     6361 gcgctccagg tacggtcggt acagcacgct gcagagtgcg cccacgccgg cgctgccgag
     6421 cacggcaagc gcgccgatcc attcgccgcg gctcgcgccg acgaggagct tttctccgag
     6481 cacgcaggcc acgccgacga tcgtcagcag cacgccgaac agcacgcgcg ccgacaaccg
     6541 ctcgcgtccc agcacggcgc cgagcaccag tgtggcaagc ggcatcgtcg cgaacagcag
     6601 accggcgcgc gcggacgcga tgaactgcag cccgtagttc atcagcgcga tcagcacgcc
     6661 gaactgcccg atgccgagca gcgcaatcgg cgcgaggtcg cgccgcgcga agcggatcga
     6721 cggcacgaag ggcgccaggc acgcgagcgc gatcgtgtag cgcagcagcg cgagcgccgc
     6781 cggcgtcgtc tcgccgacca cgaagcgcgt ggcgacgatc gcagcgccga cctgcacgcc
     6841 gaacgcggcc gcggcaagca gcggcgcgag gcgcgtcacc gcagcggca
//
LOCUS       DBNHBY010000682         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1742313, whole genome shotgun sequence.
ACCESSION   DBNHBY010000682 DBNHBY010000000
VERSION     DBNHBY010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1742313"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="AC16HB_16265"
     CDS             <1..1953
                     /locus_tag="AC16HB_16265"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR5608396.1"
                     /translation="IRENEQRARFIGFPTNNVKLFAFVASATLTGFAGMLLLFNNRMT
                     SAEPISVAFSGELLAMVIIGGMRSFLGPALGALFYVIFRDSLSRITEDWLLYFGLLFV
                     AFVVFSPTGLVGLYEKLTSRWRHRPADDAAMAKRQTGEVALPAFLKPGQHSDAPIVEV
                     QSLAKSFGGIHAVRGVQLRMRDRSLHALIGPNGAGKTTAFNLISGMFAPDAGSITLAG
                     RPIAGLPPERITQAGIGRSFQITNLFPSLTVEENVRLAVQSRDPNRFAFWVHARALDN
                     VNRETGEIVRTMGLAGVESAEAASLSYGGQRLLDMSLALATKPRVLLLDEPLAGLAAA
                     ERERVGQLIKGISRDIPVLLVEHDIDRVFALADAVTVMNDGQVLVDGSVEDARSSPRV
                     QEVYIGSGAHALAEKERPSAARESVLLALDRVNTFYGKSHILRDVSFDVRDNEIVALL
                     GRNGAGKSTLLKTLVGIAPPAEGEIRLGGDSLARRPSAEITRRGISYVPQGRGLFAGM
                     TVAENMELGRLKRRNGAGKHWDEDRIFEFFPRIRERWRSPADYLSGGEQQMVAVARAL
                     VGDTRLLLLDEPFEGLAPAVVEELFEAFDKLRQEVSILIVDHHLDLALALSDRTVALE
                     RGMVTHIGPSRALSRDFDLRRKVLWL"
     gene            1964..>2248
                     /locus_tag="AC16HB_16270"
     CDS             1964..>2248
                     /locus_tag="AC16HB_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR5608397.1"
                     /translation="MSTIAIIGAGLIGRSWANVFARANWRVRVWDPVEAQRTTAAEQI
                     TRSLFDLQKHGLVGDPAVAASNISVHATLEDAVRDADFVQESGPETVDAKR"
BASE COUNT          346 a          735 c          802 g          365 t
ORIGIN      
        1 atccgcgaga acgagcagcg tgcgcgcttc atcggctttc cgaccaacaa tgtcaaactg
       61 ttcgcgttcg tcgcctcggc cacgctgacc ggcttcgcgg gcatgctgct gctgttcaac
      121 aaccgcatga catcggccga accgatctcg gtcgcgttct cgggcgagct gctggcgatg
      181 gtgatcatcg gcggcatgcg ctccttcctc gggccggcgc tcggggccct cttctacgtg
      241 atattccgcg actccctgtc gcggatcacg gaggactggc tgctgtactt cggcctgctg
      301 ttcgtcgcgt tcgtggtctt ttcgccgacg gggctcgtgg gtctgtacga gaagctcaca
      361 tcgcgctggc gccaccgccc ggcggacgac gccgcgatgg caaagcgcca gacgggcgag
      421 gttgcgctgc ccgcgttcct gaagcccggg cagcattcgg acgcgccgat cgtcgaggtg
      481 cagtcgctcg ccaagagctt cggcggcatc cacgcggtgc gcggcgtgca gctgcgcatg
      541 cgcgaccgca gcctgcacgc gctgatcggc cccaacggcg cgggcaagac gaccgcgttc
      601 aacctgattt cgggcatgtt cgcgcccgat gcagggagca tcacgctcgc gggtcggccg
      661 atcgcagggc tgccgccgga gcggatcacg caggccggaa tcggccgctc gttccagatc
      721 accaatttgt ttccgtcgct caccgtcgag gagaacgtgc gcctcgcggt gcaatcgcgc
      781 gaccccaacc gcttcgcgtt ctgggtccac gcgcgtgcgc tcgacaacgt gaaccgcgag
      841 actggagaga tcgtgcgcac gatggggctg gccggggtcg aatccgccga agccgcgtcg
      901 ctgtcgtatg gcggccagcg cctgctcgat atgtcgcttg cgctcgcgac caagccgcgc
      961 gtgctgctgc tcgacgagcc gctcgccggg ctggccgccg cagagcgcga gcgcgtcggt
     1021 caactgatca agggcatctc gcgcgacatc ccggtgctgt tggtcgagca cgacatcgac
     1081 cgcgtgttcg cgctcgcgga cgcggtcacg gtgatgaacg acggccaggt gctggtcgac
     1141 ggcagcgtgg aggatgcgcg cagctcgccg cgcgtgcagg aggtctacat cggctcgggc
     1201 gcgcacgcgc tcgcggagaa agagcggccg tcggcagcgc gggaatcggt gctgctcgcg
     1261 ctcgaccgcg tgaacacctt ctacggcaag agccacatcc tgcgcgacgt cagtttcgac
     1321 gtgcgcgaca acgagatcgt cgccctgctc ggccgcaacg gagccggcaa gtcgacgctg
     1381 ctgaagacgc tggtcggcat cgcgccacct gccgaaggcg agatccggct cggcggggat
     1441 tcgctcgcgc ggcggccgtc ggccgagatc acgcggcgcg gaatctcgta cgtgccgcag
     1501 ggacgcggcc tgttcgcggg catgacggta gcggagaaca tggagctcgg tcgcctgaag
     1561 cgccgcaacg gcgccggaaa gcactgggac gaggacagga tcttcgagtt cttcccgcgc
     1621 atccgggagc gttggcgttc gccggccgat tacctgtcag gcggcgagca gcagatggtc
     1681 gcggtcgcgc gcgcgctagt gggcgacacg cgcctgctgc tgctcgacga gccgttcgag
     1741 gggctggcgc cggcggtcgt cgaggaactg ttcgaggcgt tcgacaagct gcggcaggag
     1801 gtgtcgatcc tgatcgtcga tcaccacctc gacctcgcgc tggcgttgtc ggaccgcacg
     1861 gtggcgctcg agcgcgggat ggtcacgcac atcgggccat cgcgcgcgct gtcgcgggat
     1921 ttcgacctgc ggaggaaagt gctgtggttg tgaaaggagt cctatgtcga cgatagccat
     1981 catcggcgcc ggcctgatcg ggcgctcgtg ggccaacgtg ttcgcgcgtg caaactggcg
     2041 cgtgcgcgtg tgggatccgg tcgaagcaca gcgcacgacg gccgccgagc agatcacgcg
     2101 gtcgctgttc gacctgcaga agcacgggct ggtcggcgat ccggccgtgg cggcgagcaa
     2161 catcagcgtg cacgcaacgc tcgaagacgc ggtgcgcgac gccgacttcg tgcaggaaag
     2221 cgggccggag acggtcgacg ccaagcgc
//
LOCUS       DBNHBY010000683         2092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1742338, whole genome shotgun sequence.
ACCESSION   DBNHBY010000683 DBNHBY010000000
VERSION     DBNHBY010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1742338"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC16HB_16275"
     CDS             complement(<1..146)
                     /locus_tag="AC16HB_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5608398.1"
                     /translation="MPKLVLSLDGVVLREVNLSKDRMTIGRRSHNDLVIDNLAVSGEH
                     AVVFR"
     gene            complement(282..1067)
                     /locus_tag="AC16HB_16280"
     CDS             complement(282..1067)
                     /locus_tag="AC16HB_16280"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR5608399.1"
                     /translation="MVATIEYALCSHAGLVRPLNEDAVGLDPSLGLLVLADGLGGYNA
                     GEVASTMAVSSLLTQLTDDLAAARSAGVDLDVKRTLSQRLVEMNSTIFRAATSSSAYE
                     GMATTVVVAWLLDGRLWLAHTGDSRAYRFRDGVLEQLTRDHSFSQELLDAGMVTEEEA
                     RLLPAKNLVTRALGAAADVEPEVHEYPLQENDWLMLCSDGLTEMVSHAEIGAALFAAL
                     PDSQRAAQRLVDLANAAGGRDNTSIVIARALPGGTRAVTDGVD"
     gene            complement(1130..1840)
                     /locus_tag="AC16HB_16285"
     CDS             complement(1130..1840)
                     /locus_tag="AC16HB_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR5608400.1"
                     /translation="MELVEIAGTALFWKSLLEIIVVNIILSGDNAVVIALAARSLPRR
                     QQKAAIFLGSAAAIVLRVLLTVFAVQLLQYPYLKLVGAALLLWIGAKLLLPEDDGGEE
                     AAASSGLWSAVRTILIADLVMSLDNVIAVAAAAETGPPPARLTLLVIGLGLSIPLIIV
                     GSQILLKLMAKWPIIITLGAALLGFVAGEMAVTDPAIEAWVHQSFGNIDYAVSVACAV
                     LVVVIGRFWARQIEAGAQ"
     gene            complement(1898..>2092)
                     /locus_tag="AC16HB_16290"
     CDS             complement(1898..>2092)
                     /locus_tag="AC16HB_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5608401.1"
                     /translation="HMKKPVVGFIAGVTAPPGKRMGHAGAIISGGKGTAQEKLAVMEA
                     CGIRTTKNPSEIGKLLKSVI"
BASE COUNT          359 a          730 c          654 g          349 t
ORIGIN      
        1 cggaacacga ccgcgtgctc gccgctgacc gcaagattgt cgatgacgag gtcgttgtgc
       61 gaacggcggc caatcgtcat ccggtccttc gacaggttga cctcgcgcag cacgactcca
      121 tcgaggctca acaccaactt cggcatcctg tctccgtggg ccagatcgat tgttatgttc
      181 gatgcacaac gacagtcgac gaacgtccgc cgccgtttca gtcgaacaaa tctaccagcc
      241 gttcaatgag ttggccagcc gttcggcacg cgcacactgc ttcagtcaac tccgtccgtc
      301 acagcgcgcg tgccacccgg cagcgcgcgt gcgatgacga tcgaggtgtt gtcgcggcct
      361 ccggcggcat tggcgagatc gacgagccgc tgcgcggcgc gctgcgagtc gggcagcgcg
      421 gcgaacagcg cggcgccgat ctcggcatgc gacaccattt ccgtcaatcc gtccgagcac
      481 agcatcagcc agtcgttttc ctgcaatggg tactcgtgca cctcgggctc gacgtccgcg
      541 gcggcgccga gcgcgcgcgt caccaggttc ttcgccggca gcagccgcgc ctcttcttcg
      601 gtgaccatgc cggcatcgag cagttcctgc gagaacgagt ggtcgcgcgt cagttgctcc
      661 agcacgccgt cgcgaaatcg atacgcgcgc gagtcgccgg tgtgcgcaag ccacagtcgc
      721 ccgtcgagca gccacgcgac cacgacggtc gtcgccatgc cctcgtaggc gctgctgctc
      781 gtcgccgcgc ggaagatcgt cgagttcatc tcgaccaggc gctgtgacag ggtgcgtttc
      841 acgtcgagat cgacgcccgc actgcgcgca gcggcgagat cgtcggtgag ctgggtgagc
      901 aggctgctca ccgccatcgt gctggcgact tcgccggcgt tgtagccgcc caggccgtcg
      961 gccaggacca acaggccaag cgatggatcg aggcccactg cgtcttcgtt cagcgggcgc
     1021 acgagcccgg cgtgtgagca cagcgcgtat tcgatggtcg cgaccatggg catcgatcgg
     1081 tcaggaggcg gccattatca acgaaccgcg ggcgccggca cgcatctatt cactgcgccc
     1141 ctgcctcgat ctggcgcgcc cagaatctgc cgatcacgac caccagcacc gcgcaggcca
     1201 cggataccgc gtagtcgatg ttgccgaagc tctggtgaac ccaggcttcg atcgccggat
     1261 cggtgacggc catctcgccc gccacgaacc cgaggagcgc cgcgccgagc gtaatgatga
     1321 tcggccactt ggccatcagc ttcagcagga tctggctgcc gacgatgatc agcggaatcg
     1381 acagtccgag accgatcacg agcagcgtca ggcgcgcggg cggcgggccg gtctcggcgg
     1441 ctgcggcgac cgcgatcacg ttgtccaggc tcatgaccag atcggcgatc aggatcgtgc
     1501 gcacggcgct ccaaaggcct gaagatgccg cggcctcctc accaccgtcg tcttccggca
     1561 gcagcagctt ggcgccgatc cacagcagca gcgccgcgcc gacgagtttc agatacgggt
     1621 actgcagcaa ctgaaccgca aagaccgtca gcagcacgcg cagcacgatc gccgccgcgc
     1681 tgcccagaaa gatcgcggcc ttctgctgcc gccgcggcag actgcgcgcc gcgagcgcga
     1741 tgacgaccgc gttgtccccg gagaggatga tgttgacgac gatgatctcg agcagcgact
     1801 tccagaacag cgccgtgccg gcgatctcga ccaactccat ccctacccct tggattcttc
     1861 tcgttcggcc ggatccggcc gcgcgttcca gccggtccta gatcaccgac ttcagcagct
     1921 tgccaatctc cgagggattt ttcgtcgtgc ggataccgca ggcctccatg acggccagct
     1981 tctcctgcgc cgtgcccttg ccgcccgaga tgatcgcgcc cgcgtgcccc atgcgcttgc
     2041 ccggcggcgc ggtcacgccg gcgatgaagc cgaccacggg cttcttcatg tg
//
LOCUS       DBNHBY010000684         3973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_592055, whole genome shotgun sequence.
ACCESSION   DBNHBY010000684 DBNHBY010000000
VERSION     DBNHBY010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_592055"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..2127
                     /locus_tag="AC16HB_16295"
     CDS             400..2127
                     /locus_tag="AC16HB_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608402.1"
                     /translation="MSSKSAHSAARAAFLAAREVLWTHRTDLDAAVREFRWPKLTHFN
                     WALDHFDAMAEREDRTGLWIVNEDGSEQKRTFGELSRRSNQVANFLRSQGVRRGDRIL
                     LMLGNEVALWECMLGAFKLGAVLVPTTALLSVDALRDRIERGHIRHVIAAAAQAEKFA
                     SFGGLTARICVGAVRADWVSFADSTAASPSFEVDGPTAADDPLLLYFTSGTTSLPKMV
                     LHTHQSYPVGHLSTMYWLGLKPGDVHLNVSSPGWAKHAWSCFFAPWNAGACVFIHNSA
                     RFTATALLETLARCGVTSLCAPPTVWRMLIQEDLAPYRNRLKLREVTGAGEPLNPEII
                     DRVRDAWGLALRDGYGQTETTAQIGNAPGQRLKPGSMGRPLPGYEVVLLDADDRPAEE
                     GEICLKIDPRPVGLMTGYEDSPGKTADVMRNGYYHTGDVATRDSDGYLTFVGRADDVF
                     KASDYRISPFELESALLEHPAVTEVAVVPSPDPLRLAVPKAFLTLAAGVQADAALALD
                     VLAFARKRLSPFQRVRRIEFVAELPKTVSGKIRRVDLRRLEADRRGRGVRDPGEHFED
                     DFPELKSAS"
     gene            complement(2229..>3973)
                     /locus_tag="AC16HB_16300"
     CDS             complement(2229..>3973)
                     /locus_tag="AC16HB_16300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5608403.1"
                     /translation="SGRPRSKLPILVILLLIVGATLAGGAYYLWPRFESEPPRIAVSP
                     NVDALGLAPVEIQVTDKGTGLKSVTVTLSQGDAQRSLAADQFAQPVSEKKVTVALAKV
                     SGVKEGPAVLRVTARDASLWRGNEAVLEKNVTIDITPPTLELIADDRYVNFGGVGVIV
                     YKASADTAASGVKIGNYFFPGFAGQVKDHPDHFLAFFAHPYDASPEARPTLVATDQAG
                     NTREMRLAYELKNVRYKKSTIALSDAFLQNKVAPLLNDVALRQGPVKDLFVGVIVYKA
                     SADTAASGVKIGNYFFPGFAGQVKGHPDHFLASFAHPYDASPEARPTLVATDQAGNSR
                     EMRLAYELKNVRYKKSTIAITDGFLQNKVAPLLTDVAVRQGTPKDVFVAVNKQLRKEN
                     EDRITAVTKKATPVLLWKDAFAQLSNSKVEANFADQRTYTYNGEPIDTAYHLGYDLSV
                     TKNYPAEAANSGTVAFTGDLGIYGNTVIVDHGLGLFTLYGHLSSFDVKAGDSVAKRQV
                     LGKTGETGLAAGDHLHFGVYLDGVAVLPVEWWDQKWIDDNITPKLEGRSGQEIAEAQQ
                     PVKKMRKGGGKRRR"
BASE COUNT          633 a         1287 c         1355 g          698 t
ORIGIN      
        1 tcgcgggtcg gggcaggagc aaccgcggca ggcggaagca ggtctgatcg gacatgttcg
       61 acattcaggg agagaaaccg gacatggtac cgatcgcgcg caggcgcgtc ggggacgcca
      121 ggcagggccg acgtccgcat tggctcgccg agtggttggc gcgttggcct cggcagcagg
      181 tcgtattcga atgcgtgatg cgaatcctgg tcgcagtggc cgacgccagc gcagtcacgt
      241 gcgggcagaa gcgggcccgc cgttgcggtg aaacttcgca caccggtatg ctgaacgaaa
      301 cgcggcagtg accggcactt cggctccgaa cagcagtcga gcgcggccgg cgcgatctac
      361 tcgacggttc gtcacggcaa tcttgttcag gagacagcaa tgtcatcgaa atcggcgcat
      421 agcgcggccc gcgcggcgtt tctcgcggcg cgcgaagtgc tgtggaccca tcggaccgac
      481 ctcgacgccg cggtgcgtga attcagatgg ccaaagttga cgcacttcaa ctgggcgctc
      541 gatcacttcg acgcgatggc cgaacgcgaa gaccggaccg gattgtggat cgtcaacgag
      601 gacggctcgg agcagaagcg cacgttcggc gaactctcgc ggcgttcgaa ccaggttgcc
      661 aattttctgc gctcccaggg cgtgcgacgc ggcgaccgca tcctgctgat gctgggcaac
      721 gaagtggcgc tctgggaatg catgctcggc gcgttcaagc tcggcgcggt gctggtgccg
      781 acgaccgcgc tgctgtcggt cgacgcgctg cgcgatcgga tcgaacgcgg gcacatacgc
      841 cacgtgatcg cggcggccgc gcaggccgag aagttcgcaa gcttcggcgg actgacggcc
      901 cgcatctgcg tcggtgccgt gcgcgcggac tgggtgtcct tcgccgacag cacggcggcg
      961 tcgccgagtt tcgaagtgga cggcccgacc gcagccgacg atccgctgct gctgtatttc
     1021 acgtcgggca ccacctcgtt gccgaagatg gtgctgcaca cgcatcagag ctacccggtc
     1081 ggtcacctgt cgaccatgta ctggctgggc ctcaaaccgg gcgacgtgca tctgaacgta
     1141 tcgtcgccgg gatgggccaa gcacgcctgg agttgcttct tcgcgccgtg gaacgcgggc
     1201 gcctgtgtgt tcatacacaa ctcagcgcgc ttcacggcca ccgcgctgct cgagacgctg
     1261 gcgcgttgtg gcgtcacgtc gctgtgcgcg cccccgaccg tctggcggat gctgatccag
     1321 gaagacctcg ccccgtaccg gaaccgcctg aagctgcgcg aggtgaccgg agcgggcgag
     1381 ccgctgaacc ccgagatcat cgaccgcgtc cgcgatgcct ggggactggc gctgcgcgat
     1441 ggctacggcc agacggagac caccgcgcag atcggaaacg cgcccggcca gcgactgaag
     1501 ccgggatcga tggggcgccc gctgccgggc tatgaagtcg ttctgctgga cgccgacgat
     1561 cggccggccg aagagggcga gatctgcctg aagatcgatc cgcggccggt cggtctgatg
     1621 accggctatg aggacagccc cgggaagacc gcagacgtca tgcgcaacgg ctactaccac
     1681 acgggcgacg tcgccacgcg cgacagcgac ggttatctga cgttcgtcgg ccgtgccgat
     1741 gacgtattca aggcatccga ttaccgcatc agtccgttcg agctcgaaag cgcattgctc
     1801 gagcaccctg ccgtgacgga ggtcgctgtc gtacccagcc ccgatccgct gcggctcgcg
     1861 gtgcccaagg ccttcctcac gctcgccgcg ggagtgcagg ccgatgccgc gctggcgctg
     1921 gacgtcctgg ctttcgcacg gaagcgcctg tccccatttc agcgtgtgcg ccgcatcgag
     1981 ttcgtcgcgg agcttcccaa gaccgtgtcc ggaaagatcc gccgcgttga cctgcgccgc
     2041 cttgaggccg atcgacgcgg gcgcggcgtg cgcgatccgg gggaacactt cgaggatgac
     2101 ttcccggaac tgaagtccgc gtcgtagcaa tgtcagcgcg gtgagcgtgc gcctgccggc
     2161 cttccgcgcg cggcgtcccg aaatctcttc gtttacgccg ggcggttggc cactgccgcg
     2221 gcgcgcggct atcgccgccg cttccctccg cccttgcgca tcttcttcac cggctgctgc
     2281 gcctcggcga tctcctggcc gctgcgcccc tcgagcttgg gcgtgatgtt gtcgtcgatc
     2341 cacttctggt cccaccactc cacgggcagc accgcgacgc cgtcgagata gacgccgaag
     2401 tgcaggtggt cgccagcggc aagccccgtc tcgcccgtct tgccgaggac ctgtcgcttg
     2461 gcgacggaat ctcccgcctt gacgtcgaac gagctgagat gcccgtagag cgtgaagagg
     2521 ccgaggccat ggtcgacgat cacggtgttg ccatagatgc ccaggtcgcc cgtgaatgcc
     2581 accgtgccgc tgttggcggc ctcggccgga tagttcttcg tgaccgagag gtcgtagccg
     2641 aggtggtacg cggtgtcgat cggttcgccg ttgtaggtgt atgtgcgctg gtccgcgaag
     2701 ttcgcctcga ccttcgagtt gctgagctgg gcgaaggcgt ccttccacag cagcacgggt
     2761 gtggccttct tcgtgaccgc ggtgatgcgg tcctcgttct ccttccgcag ctgcttgttc
     2821 acggcgacga agacatcctt cggcgtgccc tggcgcacag cgacgtcggt gagcagcggc
     2881 gccaccttgt tctggaggaa gccgtccgtg atcgcgatgg tgctcttctt gtagcggacg
     2941 ttcttcagct cgtaggcgag ccgcatctcg cgtgagttgc cggcctggtc ggtggcaacc
     3001 agcgtgggcc tcgcctccgg cgaagcgtcg tacggatgcg cgaaggaggc gaggaagtgg
     3061 tccggatggc ccttcacctg gccggcgaag cccgggaaga agtagttgcc aatcttcacc
     3121 ccgctggccg cggtgtccgc cgacgcctta tagacgatca cgccgacgaa cagatccttg
     3181 accgggccct ggcgcagggc cacgtcgttg agcaagggcg cgaccttgtt ctgcaggaag
     3241 gcatcgctca gcgcgatcgt gctcttcttg tagcgcacgt ttttcagctc gtaggcgagc
     3301 cgcatctcgc gcgtattgcc ggcctggtcg gtggcaacca gcgtgggcct cgcctccggc
     3361 gaagcgtcgt acggatgcgc gaagaaggcg aggaagtggt ccggatggtc cttcacctgg
     3421 ccggcgaagc ccgggaagaa gtagttgccg atcttcaccc cgctggccgc cgtatccgcc
     3481 gacgccttgt agacgatcac gccgacgccg ccgaagttca cgtagcggtc gtcggcgatg
     3541 agttccagcg tgggcggcgt gatgtcgatc gtgacgttct tctccagcac ggcctcgttg
     3601 ccgcgccaca gcgacgcatc acgtgcggtg acgcgaagca cggccgggcc ctccttgacg
     3661 ccggaaacct ttgccagcgc caccgtgacc ttcttttcgc tcacgggctg cgcgaactga
     3721 tcggcggcga ggctgcgctg ggcgtctccc tgggacagcg tcaccgtgac cgacttcagg
     3781 cccgtcccct tgtcggtgac ctggatctcg actggcgcca ggcccagcgc gtcgacattc
     3841 ggcgaaacgg caatccgcgg cggctcggat tcgaagcgtg gccacaggta gtaggcgccg
     3901 cccgcaaggg tggcgccaac gatcagcagg aggatgacga ggatggggag cttgctgcga
     3961 ggtctaccgg agg
//
LOCUS       DBNHBY010000685         4462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_455934, whole genome shotgun sequence.
ACCESSION   DBNHBY010000685 DBNHBY010000000
VERSION     DBNHBY010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_455934"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16HB_16305"
     CDS             complement(<1..544)
                     /locus_tag="AC16HB_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5608404.1"
                     /translation="MAVLWLLQWAQPLLLPIAIAIAFTFLLAGPVRWLRRIGIPPPLG
                     AGVVVAALLGALTLMVTLLAQPAADWMERAPATLRQMTDAAARLRDVVVPDRAAPPRA
                     GRRNAAQAPADPISERIATEGVTITRAVIGRFASFALSGAATVILLYFFLTSEYWLLS
                     RTVEALPRRRARALLLGGVRQ"
     gene            complement(810..1154)
                     /locus_tag="AC16HB_16310"
     CDS             complement(810..1154)
                     /locus_tag="AC16HB_16310"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5608405.1"
                     /translation="MGQKIDSTIAKVEQQADRAASEVKKGVDAARASTGETVDAVGKK
                     VKDAAITTAVNAELARDSSLSALKIDVDTSNGRVFLRGTAPDAQARARATQLALRVDG
                     VVSVVNELQVRS"
     gene            complement(1302..1619)
                     /locus_tag="AC16HB_16315"
     CDS             complement(1302..1619)
                     /locus_tag="AC16HB_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5608406.1"
                     /translation="MFTRTALAAAVTALSLAILPGCAVTRGQSTVGEYIDDTTVTTTV
                     KARFIENKDVDATSIRVETLNGTVLLSGFAKSDLEKDTAERIARRVKGVKEVRNEIVV
                     RPA"
     gene            complement(1721..1921)
                     /locus_tag="AC16HB_16320"
     CDS             complement(1721..1921)
                     /locus_tag="AC16HB_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HIR5608407.1"
                     /translation="MNRANHPVKEAAMLLYATVLFVSALVAALFGFTGIAGGAVSIGR
                     HLFVLFAVLAFTVFLIGLAHGT"
     gene            complement(2302..2841)
                     /locus_tag="AC16HB_16325"
     CDS             complement(2302..2841)
                     /locus_tag="AC16HB_16325"
                     /inference="COORDINATES: protein motif:HMM:NF015331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5608408.1"
                     /translation="MWALLPLAAWCSDTDRDIVVQVQKSGQTVAVEVDCPVDAPWSVV
                     WEVLTDYDHMAQFISNLDYSGVDERVDNRLRVHQKGKVSRGFFTMAFDNVREIELFPY
                     TEIRSELISGDMKASSFVTRIMAVAGRVHILNSGRYTPNLWVPPLIGPALIEAEVRKQ
                     FDEIRTEVLRRRALLGARN"
     gene            complement(2970..>4462)
                     /locus_tag="AC16HB_16330"
     CDS             complement(2970..>4462)
                     /locus_tag="AC16HB_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR5608409.1"
                     /translation="ALTRPGQTRIAKQLAVAKAEHPGTSGFKLLPVGIDSFLLRMEMA
                     GAAERTLDVQYFLIQSDDTGQLLIGELLKAAERGVRVRVLLDDAGSFGRDAPIRALAG
                     YPNIELRLFNPFAYRGNVELLHYAELVADAARLNYRMHNKLFVVDNEIGLVGGRNIGD
                     EYFQGGLDFEFGDYDVIAAGPIVREISKSFDAFWNSPMAIPIAAFPGDRPSPQDLEKY
                     RSTLDAHRAYMVAANAPYMRKLAAGEPLAAMLSGKTSLVWSRAELIYDSPEKAKVQNG
                     EQGGRLLRNRLGEVSWKVNSELIIVSPYLVPGPAGMKFFEELRERNVRVRILTNSLAS
                     SDVPIVHSGYQAVRAPLLEKGVELYEVRPALGQPVVRGGPLKSSSSGRYALHAKVFVF
                     DRQRVFIGSMNLDQRSLHINTEIGLIIDSPELARQIAQRFADIAQPANSYVLTLGDAD
                     ANGQRRLVWRTLEDGRPVDYDHEPATTLWQRLQVEMLSILPIDGLL"
BASE COUNT          820 a         1421 c         1439 g          782 t
ORIGIN      
        1 cctggcgcac gccgccgaga agcagcgcgc gcgcgcgccg ccgcggcaag gcctcgaccg
       61 tgcgagacag cagccagtac tcggacgtga ggaagaagta caagaggatc accgtggcgg
      121 cgcccgatag cgcgaacgag gcaaagcggc cgatcaccgc gcgggtgata gtcacgccct
      181 cggtcgcgat ccgctcggag atcgggtcgg cgggggcctg ggccgcgttg cgccgacccg
      241 ctcgcggcgg tgccgcgcgg tccggcacga ccacgtctcg caagcgagcg gccgcatcgg
      301 tcatctgtct gagcgtcgcg ggcgctcgct ccatccagtc ggccgccggc tgggcgagca
      361 atgtcaccat cagcgtgagc gcgcccagca gcgctgcgac cacgacgcct gcgcccagcg
      421 gcggcgggat cccgatccgg cgcaaccacc gtaccggccc ggccagcagg aaggtgaagg
      481 cgatcgcaat cgcgatcggc agcagcagcg gctgcgccca ttgcagaagc cacagcacgg
      541 ccacgccgaa gagtccgatc acggcccagc ggcctgacat tgccgccggg aacgtgcgcg
      601 cctgcggatt cgcgacagac gcctcggtca cggaccgctc ggagaaacaa ggcgcccgtt
      661 gcccgcggag cggcgatgct tgccgtcatc acgagaggcc caagaacgtg ggcggcggca
      721 cggcgcccgg cagcacctcg gtctcccgga gaccgaggcg caaccgagaa tccggacgat
      781 gatcaagtca tcggattccg gcacgtcact caactgcgaa cctgcagttc gttgacgacg
      841 ctgactacgc cgtccacgcg cagggccagt tgcgtggcgc gcgcgcgggc ctgcgcatcg
      901 ggggcggtgc cgcgcaggaa gacgcgaccg ttgctggtat cgacgtcgat cttgagcgca
      961 ctgagcgaac tgtcgcgcgc cagttcggca ttcacggccg tcgtgatggc ggcatccttg
     1021 accttcttgc cgacggcatc gaccgtttca ccggtactgg cgcgggccgc atccacgccc
     1081 ttcttcacct cggacgcggc ccgatcggcc tgctgctcga ccttggcgat cgtcgagtcg
     1141 atcttctggc ccacggtgcg atcctcctgc ttgcggtcgc aggcggcgag gctcagaacg
     1201 gtcgtcaagg ccaatgcggc agataagagc ggaagttgtc gaactctcat gagaggctcc
     1261 ttaagtcgaa gtggtcgagt gcgcgcgtga ggcggaaacc gtcaggcagg gcgaaccacg
     1321 atctcgtttc tgacctcttt cacgcccttc actcgtcggg caattctttc ggccgtgtcc
     1381 ttctccaggt cgctcttggc gaagccggac agcaggaccg tgccgttgag cgtttccacc
     1441 ctgatcgatg tggcgtccac atccttgttc tcgatgaacc tggccttcac cgtcgtggtg
     1501 accgtggtgt cgtcgatgta ttcgccgacc gtagactgtc cgcgcgttac ggcgcagccc
     1561 ggcaggatgg cgagggaaag agcggtgaca gcagcggcga gtgcggtgcg tgtgaacatg
     1621 gcagttctcc ttcggtaaga ctgactgcaa tctcggatga tcgataaggt gttcgcccgc
     1681 cctgccaccg cagggccgag ctgagcttcc gcgcccgggc tcaggtgccg tgggccaggc
     1741 cgatcaggaa gacggtgaag gccagcacgg cgaagaggac gaacagatgt ctcccgatgc
     1801 tcaccgcgcc acccgcgatg ccggtgaagc cgaagagcgc cgcgaccagc gcgctgacga
     1861 acaagacggt ggcatacagc aacatggcgg cctccttgac ggggtggttt gcccggttca
     1921 cctgtgttct tcctggaagc gattcgctgc gatgtggaaa gcttaggaag aggtggctgc
     1981 ggtcgccatg agacgaggtg acagcttggt gtcggtcaag ccgccaacgt cctgtaggac
     2041 gagacccaca gaccgtcgtg caacccgggt ggcgttttgc tccggaaatc gccgattgcc
     2101 aggcgccgat tcggccgacg accggagcgg ccgcgacctg cacccacgcg caagctcagc
     2161 ggcaattcag cgcggcaaca tctcgcctgt gcaccagcgg cagaagaact gctcgtgttg
     2221 cgagccggtg gtggacccac tgacccgtcg aggcaatgcg ctcgcgtcga cacgccgcgc
     2281 ggcggacgat gtgcggcagg gtcaattgcg cgcgccgagc agcgctcttc gtcgaagaac
     2341 ttcggttctg atctcgtcga actgcttgcg cacttcggcc tcgatcaacg ccgggccgat
     2401 caaaggcggg acccacaggt tgggcgtgta gcggccactg ttgagaatgt ggacgcgtcc
     2461 ggcgacggcc atgattcgag tgacaaagga ggaggccttc atgtcaccgc tgatcaactc
     2521 cgaacggatc tcggtgtacg gaaagagctc gatctcgcgg acgttgtcga atgccatcgt
     2581 gaagaagcct cgcgacacct ttcccttctg gtgcacgcgc aatcggttgt ccacgcgttc
     2641 gtcgacaccg ctgtagtcga ggttcgatat gaattgcgcc atgtggtcgt aatcggtgag
     2701 gacttcccac accaccgacc acggcgcgtc caccggacag tcgacctcta ccgcaaccgt
     2761 ctgtccgctc ttctgaacct ggaccacgat gtcccgatcc gtgtccgagc accaggctgc
     2821 cagcggcagc agcgcccaca agagcgtggc caggaccttc catccgatcc ggttccgata
     2881 cgccaggacc tgccgtgtca tcgtgtctga attctgcgga gttggcgtga ggccgcgttg
     2941 gacttggcag gccgtggtca ggatgtcgtt tacagcaacc cgtcgatcgg caggatcgat
     3001 agcatctcga cctggagccg ctgccagagt gtcgtggcag gttcgtggtc gtaatccacc
     3061 ggcctgccgt cctcgagcgt gcgccagacg agacggcgtt gcccattcgc gtcggcgtcg
     3121 ccaagcgtca ggacgtagct gttggccggt tgtgcgatat ccgcaaagcg ctgagcaatc
     3181 tgacgcgcaa gctcgggact atcgatgatc agtccgattt ccgtattgat gtgcagggac
     3241 cgttggtcga ggttcatcga gccgatgaag acgcgctggc gatcgaagac gaacaccttg
     3301 gcgtgcaacg cataccggcc cgagcttgaa gacttgagtg ggccgccgcg tacaaccggt
     3361 tgaccgagcg ccggccggac ctcatacagt tcgacaccct tttccaggag cggtgcccgg
     3421 accgcctgat agcccgagtg aacgatgggc acgtcggacg acgcaagcga attggtgagt
     3481 atccgcaccc tgacgttgcg ctcgcgcaat tcctcgaaga acttcatccc ggccggcccg
     3541 ggcacaaggt acggagacac gatgatcaac tctgaattca ctttccacga cacctcgccg
     3601 agacgattcc gcagcaaccg acccccctgc tcgccgttct ggacctttgc cttctccggg
     3661 ctgtcgtaga tgagttccgc tcgggaccac acgagagatg tctttccact gagcatggcc
     3721 gcaagtggct cgccggcagc cagcttgcgc atgtatgggg cgttggctgc aaccatgtag
     3781 gcgcgatgcg cgtcgagagt gctgcgatat ttctcgagat cctgtggcga aggccgatcg
     3841 ccggggaatg ctgcgatcgg aatcgccatc gggctgttcc agaaagcgtc gaaacttttc
     3901 gagatctcac gaacgatcgg gccggccgcg atcacgtcgt aatcgccgaa ttcaaagtca
     3961 agaccgccct ggaaatactc gtcaccgatg ttgcgtccgc ccacgaggcc gatctcgttg
     4021 tcgacgacga agagcttgtt atgcattcga tagttgagcc gggcggcatc agccacgagc
     4081 tcggcatagt gaagaagctc gacatttccc cggtacgcga acggattgaa gagccgcaac
     4141 tcgatattcg ggtacccggc aagtgccctg atcggcgcgt cgcggccgaa cgacccggcg
     4201 tcgtcgagca ggacacgaac ccgcaccccg cgttcggcgg ccttgagcag ttcaccgatc
     4261 agcagctggc cggtgtcgtc gctttgaatg aggaagtact gcacgtccag tgttcgttcc
     4321 gccgcgcccg ccatttccat ccgcagcaga aagctgtcga tgccgaccgg aagcagcttg
     4381 aagccggagg tgcccgggtg ctcggccttt gccacggcca attgctttgc aatgcgggtc
     4441 tgtcccgggc gcgtcagagc ga
//
LOCUS       DBNHBY010000686         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_50751, whole genome shotgun sequence.
ACCESSION   DBNHBY010000686 DBNHBY010000000
VERSION     DBNHBY010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_50751"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16HB_16335"
     CDS             complement(<1..194)
                     /locus_tag="AC16HB_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608410.1"
                     /translation="MHVRLTRIMAAAALLTLTMTVAAQPKSRADCERDYKPQVGQSGK
                     DVVWVPTPDELVSRMLKMAGT"
     gene            complement(238..>2014)
                     /locus_tag="AC16HB_16340"
     CDS             complement(238..>2014)
                     /locus_tag="AC16HB_16340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5608411.1"
                     /translation="IQAAAQKWGASTERLDTKESTVVEIGGQKRRASFGSLADAAMKL
                     PVPTRVGIKADARDFTLIGKPTRRIDAREKVNGSAKFGIDQRLPGMRTAIVARPPVFG
                     GKVAKFNADKARAIKGVEAVVPVQVDRAGTGIAVIANGFWPAKQGRDALEIEWALPAG
                     PTSAEQFVQYRQLAQQPGLPARADGDANALAGAAKTITAEFEFPYLAHAPMEPLNATV
                     DLKADKLTIWCGSQFQTIDHLLAAQAAGMQPEQVELTTTFAGGGFGRRAVPTSDYVVE
                     AVRVAKAMKESGVSAPVQVIWTREDDIRGGYYRPAHVHRVAVGSDAKGMPVAWQHTIV
                     GQSIIKGTAFEKSLVKNGIDETTTEGVVDTPYRLPNLRVNVHHPEVNVPVLWWRSVGN
                     THTAFVMETLIDELAAAAKQDPIAYRLALLDAKHTRHRAVLALVREKSGWGKPLPKGR
                     ARGIALHESFGSVVAHVAEVSVAGNEVRVHRVTSAIDCGTAVNPLGVEAQVQSAMVFG
                     LTAALHGKITLKNGQVEQSNFHDYPPLRIDAMPETSVHIVPSTAAPTGVGEAGTPPIA
                     PAVANAVAALTGKRLRRLPFDLASA"
BASE COUNT          268 a          701 c          675 g          370 t
ORIGIN      
        1 gtgcccgcca tcttcagcat gcgcgaaacg agttcgtcgg gcgtcggcac ccacaccacg
       61 tccttgccgc tctggccgac ctggggcttg taatcgcgtt cgcagtcggc gcgggacttg
      121 ggttgcgcgg cgaccgtcat ggtcagcgtc agcagggccg cggcggccat gatccgggtc
      181 aggcgaacat gcatcggttt ctccctgagg ttgttgtgga cggtgcgcga ttctgcctca
      241 cgcgctggcg agatcgaaag gcaacctgcg caggcgcttg ccggtcagtg cggcgacggc
      301 attggccacg gccggcgcaa tcggcggcgt gccggcttcg ccgacgcccg tcggcgccgc
      361 ggtgctcggc acgatgtgca cgctggtttc gggcatcgcg tcgatgcgca gcggcgggta
      421 gtcgtggaaa ttgctctgtt cgacctgtcc gttcttcagc gtgatcttcc cgtgcagcgc
      481 cgccgtcagt ccgaacacca tcgccgactg cacctgcgct tcgacgccga gcggattgac
      541 ggccgtgccg cagtcgattg cggaggtcac gcggtgcacg cggacctcat tgccggcgac
      601 cgagacttcg gcgacgtgcg cgaccaccga gccgaagctt tcgtgcaggg cgatgccgcg
      661 cgcgcggcct ttcggcagcg gcttgcccca gccggacttc tcgcgcacga gcgcgagcac
      721 cgcgcgatgc cgcgtgtgct tggcgtcgag cagagcgagg cggtaggcga tcggatcctg
      781 cttcgcggca gcggcgagtt cgtcgatcag ggtttccatc acgaacgccg tgtgcgtatt
      841 gccgaccgag cgccaccaca ggaccggcac gttcacttcg gggtgatgca cgttcacgcg
      901 caggttgggc agccggtatg gcgtgtcgac cacgccttcg gtcgtcgtct cgtcgatgcc
      961 gttcttgacc agcgacttct cgaatgcggt gcccttgatg atcgactgtc cgacgatcgt
     1021 gtgctgccag gcgaccggca tgcctttcgc gtcgctgccg accgccacgc ggtgcacgtg
     1081 cgcgggccgg taatagccgc cgcggatgtc gtcttcgcgc gtccagatca cctgcacggg
     1141 tgcgctgacg ccggattcct tcatcgcctt tgccacgcgc acggcctcga ccacgtagtc
     1201 ggaggtcggc accgcgcggc ggccgaaccc gccccccgcg aacgtggtgg tcagttcgac
     1261 ctgctccggt tgcattcctg cggcctgcgc cgccagcagg tggtcgatcg tctggaactg
     1321 cgacccgcac cagatcgtca gtttgtccgc ctttaggtcg accgtcgcgt tcagcggctc
     1381 catcggggcg tgcgccagat aggggaactc gaactcggcc gtgatcgtct tggccgcgcc
     1441 ggcgagcgcg ttggcgtcgc cgtcggcacg cgccggcagg cccggttgct gcgcgagttg
     1501 tcgatactgc acgaactgct cggccgacgt cggcccggcc ggcagcgccc attcgatctc
     1561 gagcgcgtcg cggccctgct tcgccggcca gaagccgttg gcaatcacgg cgatccccgt
     1621 gccggcgcga tcgacctgca ccggaacgac cgcctcgacg cccttgatcg cacgcgcctt
     1681 gtccgcattg aacttcgcga ccttgccgcc gaacaccggc ggccgcgcga cgatcgcggt
     1741 gcgcatgccg ggcaggcgct ggtcgatgcc gaacttcgcg ctcccattga ccttctcgcg
     1801 tgcatcgatg cggcgcgtcg gcttgccgat cagcgtgaag tcgcgcgcgt cggctttgat
     1861 tccgacgcgt gtcggcaccg gcagcttcat cgccgcatcg gcaagcgaac cgaagctcgc
     1921 acgccgcttc tgtccgccga tctcgacgac cgtcgactcc ttggtatcga gcctttcggt
     1981 cgacgcgccc cacttctgcg cggcggcctg gatc
//
LOCUS       DBNHBY010000687         7951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_254153, whole genome shotgun sequence.
ACCESSION   DBNHBY010000687 DBNHBY010000000
VERSION     DBNHBY010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_254153"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="AC16HB_16345"
     CDS             <1..788
                     /locus_tag="AC16HB_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5608412.1"
                     /translation="LAVYCAGTEAPVTLADVDRQYSTQAWLKRRAAQRLPARVGIRGR
                     ALQPNTVVRPPAGFAHMALDTPGGLRGFDLMRVVMYADAILMPVGASVFDRESTAECF
                     AELRKLPRVASGRCKVAAVGMRIDARTSAEDLLRRWAQALQLPFLGVLRETQAYVRTV
                     ECGLTLFDLPAAKVESDMRQWQPVLEWLDPILEAAPSMPTLSRPTSPLTLPRAAEAEI
                     TRAPAPATHDEAAVAVPAGRPSIATRLGQLIETLAVPRFLQRN"
     gene            906..3653
                     /locus_tag="AC16HB_16350"
     CDS             906..3653
                     /locus_tag="AC16HB_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5608413.1"
                     /translation="MHPEKNAQTANARYVPTYCYNCVAGPDLLTVKVANGVATEIGPN
                     FAGVGVHPSDGRPCVKAYGLIQKTYHPARILTPMKRTNPKKGIDEDPRFVPISWDEAL
                     DTVAAKLAAIRAKGPLDELGLPRIAATFGHGGTPANYMGTFPAFLSTLGAIDYSFGSG
                     QGVKCVHSEHLYGEYWHRAFTVSADTPLCNFNISLGNNNEVTGGPCATWRHASARVRG
                     MKRVQIEPHLSITGAASAEWVPIKPKTDGACMFALIHVMLHEHPRERLDLAFLRDMTS
                     SPYLVGPHGFYLRDPESGKPLLWDSQRDAAVPFDTPGVVPLLEGSRRVASAVELGADD
                     ERWTCADVVAETAFTKLVVHMRQYSPEWAEKICEVLAAKIRKIAGEYLDNACIGQTIE
                     VNGETLPYRPVAVTLGKTVNNGWGAFECVWSRTVLAVLVGALEVPGGTLGTTIRLNKP
                     HEDRHASVKPGPDGFMLNDLNPTTKGKWALGQGGRNAHRTLVPLVKNSGAWSQSLGPT
                     HLAWMFQKETPEHWPASSKPEIWFAYRTNPAISFWDTRAVAQTMAQFPFTVCFAYTPD
                     ETNHMADILLPEATDLESTQLIRLGKTKYVEQFWEHEGFVLRQAAVQPAGDTRDFTWI
                     STELARRTGLIESYNDAINRGACGVTLRNDRRDFALDPAQAHPADAIWDAVCKAASHE
                     ISQGAEVHDLAWFKEHGFMARPFKHASWYLYPTLKKKGLRFELPYQERILRSGRELGN
                     RMHETEIRWWDRQLREYQALPVWHDFPQMWEDDLVRRGLKPEDFPFWLITTKSMQYHS
                     GGNAAIQLMDEVSTNLRGHRAVVINAATAEKLGIEEGDVVEVASAIGTTQGPAMPVQG
                     IRPDTLLIVGQFDHWATPYAKDMKAPSLNTIAPMSLELTDATGSGADVVRVNVRRAVH
                     A"
     gene            3650..4120
                     /locus_tag="AC16HB_16355"
     CDS             3650..4120
                     /locus_tag="AC16HB_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxylaminobenzoate lyase"
                     /protein_id="HIR5608414.1"
                     /translation="MTLDEFRPLLRPVTDALAGRTLTPALGDELDRRIPPSSVEFKAI
                     EAACHAAIAAGWMCAQGGAGRRFGRVIEPSAQTANLSVDVVDLTDIVGPHHRHPNGEV
                     CMVMPVTPTARFDGKGAGWKVYPPSSAHRPTVTNGRALVLYLLPDGKIEFTGQA"
     gene            4203..5717
                     /locus_tag="AC16HB_16360"
     CDS             4203..5717
                     /locus_tag="AC16HB_16360"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5608415.1"
                     /translation="MNLACYLEQHAAADPDRIAVRFEGRSITYGGLNRDAARLADALR
                     RQGIGTGERVALFLPNIPEFITAYYAAQKLGAIVVTINAIFKTEEVRYLLDDSGAAAL
                     FTTAELSKFVPPDCKALRLRVLVDGAAPGWAALDGLLAQGADGFASVPREANDDAALL
                     YSSGTTGFPKGVVLTQHNIHSNTALAARYSNYRPGDRLALFLPLFHVYGQNYIMNAAM
                     LAGSTVVLIRRFVPDVVLAAIGAERITHFFAVPTIFIALLAMDLSRFDLSSLRYEMSA
                     AATMPEEISRRWTERFGRRVYEGYGLTECSPFACYNDVQEHRFGSVGRAVEGFELAIF
                     DELDRALPPGESGEIVIRGDGVMKGYWNRPDETARALRGGWLHSGDMGRMDKDGYVYI
                     VDRVKDMINISGFKVWPAEVEQFLYRMPGVLEAAVYGIPDAQQGEQVAVALVPRPNSK
                     VTSQDVIDYCRQNIAAYKVPALVEIVSELPKSPSGKLLKRILRERHAAQRGGKA"
     gene            5748..6884
                     /locus_tag="AC16HB_16365"
     CDS             5748..6884
                     /locus_tag="AC16HB_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608416.1"
                     /translation="MKHVRTIALLAGIAIASAVQAEITVGVSVSSTGPAASLGIPEKN
                     TVLLLPAAIGAEKLKYVLLDDATDPTAAVRNARKFVSEDKVDVIIGSTTTPTSMAMAE
                     VAAESRTALIAMAPLAPMDAARQAWVFQTPQGIGVMASAITDHMAAAGVKSVGFIGYA
                     DAYGEVWLKAMTAALEKKSIKLAAVERYQRTDTSVTGQALKIVGANPDAVLIAGSGTP
                     AVLPQATLVERGYKGRFYQTHGIANNDFLRVGGKNVEGTIFPVGPILVAEQLPDSHPS
                     KKPALDYIKAYEGAHGAGSRSTFGAHAYDAFLLLQDAVPRALAKAKPGTAEFRQALRD
                     AIEATRDLPVSHGVFTMSPSDHNGMDHRSRVMVRIDNGGWKFVP"
     gene            complement(6899..7819)
                     /locus_tag="AC16HB_16370"
     CDS             complement(6899..7819)
                     /locus_tag="AC16HB_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5608417.1"
                     /translation="MNFRQLDLNLLRVLAAVYRTGSVTEAGRQLALSQPATSNALARL
                     REFFRDELFVRSPRGLHPTRTAQRVAPAVIAQLQALESAVQSAEAFDPATSRAQWRLS
                     LSDLGEILFLPPLAEALRRDAPGAQISNVAVAAERVSAALEARDIDLAIGILQPAHSG
                     IASESLFQERFVAIIAPGWTPPLGRAGRSLNAKQLAGAALAVAAPTATFHGGIELMLR
                     RLKLADRPVLRARHYGALPEIVSRTDLLALIPLMYAQSLAPRYELRVWELADLAPAYD
                     VRLLWHQSVTGDPAHAWLRGVLRRLFKRDA"
BASE COUNT         1337 a         2643 c         2790 g         1181 t
ORIGIN      
        1 accttgccgt ctactgcgct ggaaccgagg cgccggtgac actggccgac gtcgatcgcc
       61 agtattcgac gcaggcgtgg ctcaagcgcc gcgccgcgca gagactgccg gcacgggtcg
      121 gcatcagggg acgcgccctc cagccgaaca ccgtcgtgcg gccgccggcg ggattcgcgc
      181 acatggccct cgatacgccg ggcgggctgc gcggcttcga cctgatgcgc gtggtgatgt
      241 acgccgacgc gatcctgatg cccgtcggcg cgtcggtgtt cgatcgcgaa tcgaccgccg
      301 aatgcttcgc cgaactgcgc aagctgccgc gcgtggcgag cggccgctgc aaggtggccg
      361 ccgtcggcat gcggatcgac gcccgcacca gcgcggaaga cctgctgcgc cgctgggcgc
      421 aggcgttgca gttgccgttc ctcggcgtgc tgcgcgagac gcaggcctac gtgcggacgg
      481 tggagtgcgg actgaccctg ttcgaccttc cggccgcgaa agtcgagtcc gatatgcgcc
      541 agtggcagcc ggtcctcgaa tggctcgatc cgatcctgga ggccgcgccg agcatgccga
      601 cgctgagccg tccgacctcg ccgctgacgc tgccgcgcgc cgccgaagcc gagatcacgc
      661 gtgcgcccgc gccggctacg cacgatgagg ccgcggttgc ggtgccggcg gggcgtccgt
      721 cgatcgccac gcggcttggc cagctgatcg aaacgctcgc ggttccgcgg ttcctgcaac
      781 gcaactaggg tttctggatt cattgaccgc tcggtcaatg aatccggcga tttctgaccc
      841 agatcagagt cgcagcggcg gcgcggcccg aagatgggcg cctctggaga cacggggagg
      901 cgcacatgca tcccgaaaag aacgcgcaga cggcaaacgc gcggtacgtt cccacctact
      961 gctacaactg cgtcgcgggg ccggacctgc tcacggtgaa ggtcgcgaac ggcgtggcga
     1021 cggagatcgg gccgaacttc gcgggcgtcg gcgtgcatcc gagcgatggc cgcccctgcg
     1081 tgaaggcgta cgggctgatc cagaagacct atcacccggc gcggatcctg acgccgatga
     1141 agcgcacgaa cccgaagaag ggcatcgacg aggacccgcg cttcgtgccg atctcgtggg
     1201 acgaggcgct cgacacggtc gcagcgaagc tcgcggcgat ccgcgccaag gggccgctcg
     1261 acgaactcgg actgccgcgg atcgcggcga ccttcggcca cggcggcacg ccggccaact
     1321 acatgggcac gttcccggca ttcctcagca cgctcggggc gatcgactac agcttcggct
     1381 cggggcaggg tgtgaagtgc gtgcattccg agcacctgta cggcgagtac tggcaccgcg
     1441 cattcaccgt gtcggcggac acgccgctgt gcaacttcaa catctcgctt ggcaacaaca
     1501 acgaggtcac cggcgggccg tgcgcgacct ggcgccacgc ctcggcgcgc gtgcgcggca
     1561 tgaagcgcgt gcagatcgag ccgcacctgt cgatcaccgg cgcggccagc gccgaatggg
     1621 tgccgatcaa gccgaagacc gacggcgcgt gcatgttcgc gctgatccac gtgatgctgc
     1681 acgaacatcc gcgcgaacgg ctcgatctcg cgttcctgcg cgacatgacg tcgtcgccgt
     1741 acctcgtcgg gccgcacggc ttctacctgc gcgatcccga gtcgggcaag ccgctgctgt
     1801 gggactcgca gcgggacgcg gcggtgccgt tcgacacgcc gggcgtcgtg ccgctgctgg
     1861 agggcagccg gcgcgtggcc agtgcggtcg aactcggcgc cgacgacgag cgctggacgt
     1921 gcgcggacgt cgttgccgag accgcattca ccaagctcgt cgtccacatg cgccagtaca
     1981 gcccggagtg ggcggagaag atctgcgaag tgctggccgc caagatccgc aagattgccg
     2041 gcgagtatct cgacaacgca tgcatcggcc agaccatcga ggtcaacggc gagacgttgc
     2101 cgtatcgccc ggtcgcggtc acgctcggca agaccgtcaa caacggctgg ggcgcgttcg
     2161 aatgcgtgtg gtcgcgcacc gtgctcgcgg tgctggtcgg tgcgctcgag gtgccgggcg
     2221 gaacgctggg cacgacgatt cgcctgaaca agccgcacga ggaccgccat gcgagcgtga
     2281 agcccggacc cgacggcttc atgctgaatg atctgaaccc gacgacgaag ggcaagtggg
     2341 cgctggggca gggcggccgc aacgcgcacc gcacgctggt gccgctggtg aagaacagcg
     2401 gcgcatggag ccagtcactg ggaccgaccc atctggcgtg gatgtttcag aaggaaacgc
     2461 ccgagcactg gcccgcatcg agcaagccgg agatctggtt tgcctatcgc accaacccgg
     2521 cgatctcgtt ctgggacacg cgcgcggtgg cgcagacgat ggcgcagttt ccgttcacgg
     2581 tgtgctttgc gtacacgccg gacgagacga atcacatggc cgacatcctg ctgcccgagg
     2641 cgaccgacct cgaatcgacg cagctcatcc ggctgggcaa gaccaagtac gtcgagcagt
     2701 tctgggagca cgagggtttc gtgctgcggc aggcggcggt gcagcccgcg ggcgacacgc
     2761 gcgacttcac gtggatttcc acggagctgg cgcgccgcac gggcctgatc gagagctaca
     2821 acgatgcaat caaccgcggt gcgtgcggcg tgacgctgcg caatgaccgc cgcgacttcg
     2881 cgctcgatcc cgcgcaggca cacccggccg acgcgatctg ggacgcggtg tgcaaagccg
     2941 ccagccacga gatctcgcag ggggcggaag tgcacgatct cgcgtggttc aaggagcacg
     3001 gcttcatggc caggccgttc aagcacgcga gctggtacct gtacccgacg ctgaagaaga
     3061 agggcttgcg cttcgaactg ccgtaccagg agcggatcct gcgttccggg cgcgaactgg
     3121 ggaaccggat gcatgaaacc gagatccgct ggtgggaccg gcagctgcgc gaataccagg
     3181 cgctgccggt gtggcacgac ttcccgcaga tgtgggagga cgacctggtg cgccgtgggt
     3241 tgaagcccga ggactttccg ttctggctca tcacgaccaa gagcatgcag taccactcgg
     3301 gcggcaacgc ggcgatccag ttgatggacg aagtgtcgac caatctgcgc ggccatcgcg
     3361 ccgtcgtgat caatgccgcc actgccgaga agcttggcat cgaggaaggc gacgtggtcg
     3421 aggtcgcctc ggcgatcggc accacgcagg gcccggcgat gccggttcaa ggcatccgcc
     3481 ctgacacgct gctgatcgtc gggcaattcg atcactgggc cacgccgtac gcgaaggaca
     3541 tgaaggcgcc gagcctgaac acgatcgcac cgatgtcgct ggagctgacc gacgcgaccg
     3601 gctccggtgc cgacgtggtg cgggtgaacg tgcgcagggc cgtgcacgca tgacgctcga
     3661 cgagttccgt ccgctgctgc ggcccgtgac cgatgcgctt gcgggccgga cgctcacccc
     3721 ggcgctcggc gacgaactcg accgtcgaat tccgccgtcg tcggttgagt tcaaggcgat
     3781 cgaggccgcc tgccacgccg cgatcgcggc cggctggatg tgcgcgcaag gcggcgcggg
     3841 ccggcgcttc gggcgcgtga tcgaaccgag cgcgcaaacg gcgaatctca gcgtcgacgt
     3901 cgtcgacctg accgatatcg tcggcccgca tcaccggcat ccgaacggcg aggtttgcat
     3961 ggtgatgccg gtcacgccga ccgcgcgctt cgacggcaag ggcgcggggt ggaaggtcta
     4021 tccgccgtct tccgcgcacc gcccgaccgt cacgaacggg cgcgcactgg tgctctatct
     4081 gctgcccgac ggcaagatcg agttcaccgg ccaggcctga cgcagccgcg gcgcgagcgg
     4141 caaaggcggc gggcattcga gtacgctgcg agtctcaaag cgatccgtgt cggaggcgac
     4201 cgatgaacct tgcctgctat ctcgagcagc acgccgccgc ggatcccgac cgcatcgccg
     4261 tccgcttcga gggccgttcg atcacctacg gcggactgaa ccgggacgcc gcgcggctgg
     4321 ccgacgcatt gcgccggcag ggcatcggca cgggggaacg cgtggcgctc ttcctgccga
     4381 acattcccga attcatcacc gcgtactacg cggcgcagaa gctcggcgcg atcgtggtca
     4441 cgatcaacgc gatcttcaag accgaggaag tgcgctacct gctcgacgat tcgggcgcgg
     4501 cggcgctgtt cacgacggca gagctgtcga agttcgtccc gcccgactgc aaggcgctgc
     4561 ggctgcgtgt gctcgtcgat ggcgccgcac ccggctgggc cgcgctcgac ggcctgctcg
     4621 cgcagggcgc ggatggcttt gcatcggtgc cgcgtgaggc aaacgacgat gcggcgctgc
     4681 tgtactcgtc cggcacgacc ggatttccga agggcgtggt gctgacccag cacaacattc
     4741 actccaacac cgcgctggcg gcgcggtact cgaactaccg gccgggcgat cggctggcgc
     4801 tgttcctgcc gctgttccac gtctacggac agaactacat catgaacgcg gcgatgcttg
     4861 ccggttcgac ggtcgtgctg atccgtcgtt tcgtccccga cgtggtgctg gccgctatcg
     4921 gcgccgagcg catcacgcac ttcttcgcgg tgccgacgat cttcatcgcg ctgctggcga
     4981 tggacctgtc gcgcttcgac ctgtcgtcgc tgcgctacga gatgtcggcc gccgcgacga
     5041 tgcccgagga gatctcgcgc cgctggaccg agcgcttcgg tcgccgcgtc tacgagggtt
     5101 acggcctgac cgagtgttcg ccgttcgcct gctacaacga cgtgcaggag caccgcttcg
     5161 gctcggtcgg ccgcgcggtg gagggattcg agctcgcgat cttcgacgaa ctcgaccgcg
     5221 cgctgccgcc cggcgagtcc ggcgaaatcg tgatccgcgg cgacggcgtg atgaagggct
     5281 actggaaccg gcccgacgag accgcgcgcg cactgcgcgg cggctggctg cactcgggcg
     5341 acatgggacg gatggacaag gacggctacg tctacatcgt cgaccgcgtg aaggacatga
     5401 tcaacatctc gggcttcaag gtctggccgg ccgaagtcga gcagttcctg taccgcatgc
     5461 ccggtgtgct ggaagctgcc gtctatggca taccggacgc gcagcagggt gagcaggtcg
     5521 cggtcgcgct ggtgccgcgg ccgaacagca aggtcacgtc gcaggacgtg atcgactact
     5581 gccggcagaa catcgccgcc tacaaggtgc cggcgctggt cgagatcgtg tccgagctgc
     5641 cgaagagccc gagcggcaag ctgctgaagc ggatcctgcg ggagcgccac gccgcgcagc
     5701 gcggcggcaa ggcgtagatc atcacgagct acaaggagga gtcgccgatg aaacacgtac
     5761 gaacgatcgc tctgctggcc ggcatcgcga tcgcatctgc agtgcaggcc gagatcaccg
     5821 tgggcgtcag cgtttcgtcg accggacccg cggcgtcgct cggcattccg gagaagaaca
     5881 ccgtgctgct gctgccggcg gcgatcggtg ccgagaagct gaaatacgtg ctgctggacg
     5941 acgcgaccga cccgaccgca gcggtcagga acgcacgcaa gttcgtcagc gaagacaagg
     6001 tcgacgtcat catcgggtcg acgacgacgc cgacgtcgat ggcgatggcc gaggtcgcgg
     6061 cggagagcag gacggcgctg atcgcgatgg cgccgctggc gccgatggac gctgcgcgcc
     6121 aggcgtgggt gttccagaca ccgcagggca tcggcgtgat ggcttcggcg atcaccgacc
     6181 acatggctgc cgccggcgtc aagtcggtcg ggttcatcgg ctacgcggac gcgtacggcg
     6241 aggtctggct gaaggcgatg accgccgcgc tggagaagaa gtcgatcaag ctcgccgctg
     6301 tcgagcgcta ccagcgcacc gacaccagcg tgaccggcca ggcgctgaag atcgttggcg
     6361 ccaacccgga cgcggtgctg atcgccggct ccggcacgcc tgcggtgctg ccgcaggcca
     6421 ccctggttga gcgcggctac aaggggcgct tctaccagac gcacggcatc gccaacaatg
     6481 atttcctgcg tgtcggcggc aagaacgtcg aaggcacgat ctttccggtc ggcccgatcc
     6541 tggtcgcgga acagttgccg gattcgcatc cgagcaagaa gccggcgctc gactacatca
     6601 aggcgtacga gggcgctcac ggcgcgggca gtcgcagcac gttcggcgca cacgcatacg
     6661 acgcgtttct gctgctgcag gacgccgtgc cgcgcgcgct ggcgaaagcc aagcccggga
     6721 cggcggagtt ccgccaggcg ctgcgcgatg cgatcgaggc gacgcgcgat ctgcccgtgt
     6781 cgcatggcgt gttcacgatg tcgcccagcg atcacaacgg catggatcac cgctcgcgcg
     6841 tgatggtgcg gatcgacaac ggcggctgga agttcgtgcc gtaggcaccg gctccggcct
     6901 acgcgtcgcg cttgaacagc cgacgcagca cgccgcgcag ccacgcgtgc gccgggtcac
     6961 cggtgaccga ctggtgccac agcagccgca cgtcgtacgc gggggcgagg tcggccagtt
     7021 cccagacgcg cagttcgtag cgcggcgcga gcgactgcgc gtacatcagc ggaatcagcg
     7081 cgagcaggtc ggtgcgcgaa acgatctcgg gcagcgcgcc gtagtgccgt gcgcgcagca
     7141 cggggcggtc ggcgagcttc aggcgcctga gcatcagttc gatgccgccg tggaaggtcg
     7201 ccgtcggcgc ggcgaccgcg agcgccgcgc ccgccagttg cttcgcgttc agcgagcggc
     7261 cggcgcggcc gagcggcggc gtccagcccg gcgcgatgat cgcgacgaag cgctcctgga
     7321 acagcgactc gctggcgatg ccgctgtgcg ccggctgcag gatgccgatc gccaggtcga
     7381 tgtcgcgcgc ctccagcgcc gcgctcacgc gctcggccgc gaccgcgacg ttggagatct
     7441 gcgcgccggg cgcgtcgcgc cgcagcgcct cggccagcgg cggcaggaac aggatttcgc
     7501 ccaggtccga cagcgacagc cgccactgcg cgcggctggt cgcggggtcg aacgcctcgg
     7561 cactctgcac cgcggattcg agcgcctgca attgcgcgat caccgcgggc gcgacacgct
     7621 gcgcggtgcg cgtcgggtgc agcccgcgcg gtgagcgcac gaacagctcg tcgcgaaaga
     7681 actcgcgcag ccgcgccagc gcattgctgg tcgccggctg cgacagcgcg agctgccgcc
     7741 ccgcctccgt gaccgagccg gtgcggtaca cggcggccag cacgcgcagc aggttcaggt
     7801 ccagctggcg gaaattcatc tgcactccta tctgattcgt gaatgcgccg cattctgaca
     7861 atcaattgga cgaatggcga gcggcggcct agattggcgc gcacgaggag acaaacgtgc
     7921 tgcagatcat gatccagggc cgcggcggcc a
//
LOCUS       DBNHBY010000688         2485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1742405, whole genome shotgun sequence.
ACCESSION   DBNHBY010000688 DBNHBY010000000
VERSION     DBNHBY010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1742405"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="AC16HB_16375"
     CDS             complement(<1..139)
                     /locus_tag="AC16HB_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5608418.1"
                     /translation="MTQPLAGLRILDLTRLLPGPAATMHLADFGADVIKVEDTGEGDY
                     MR"
     gene            complement(136..2028)
                     /locus_tag="AC16HB_16380"
     CDS             complement(136..2028)
                     /locus_tag="AC16HB_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5608419.1"
                     /translation="MLRLIDVSLARGIRTLYSNVSLIAPPGERVGLVGANGSGKSSLF
                     AAILGELSIDAGTLEAPPPARIAHVAQDIGATGESALDYVLAGHAPLVAARVELERAT
                     AGHDDLRLAHAHARLAELNEGAIVARAKAVMHGLGFAERDGMRRVADFSGGWRNRLAL
                     ARALLRPADLLLLDEPTNHLDLDSVVWLEAWLKRQDATVLVISHDREFLDRVTTTTWH
                     VADGAIRRYAGNYTAFELAWLEQQKQQDAAAKAYERTAAHLQSYVDRFRAKATKARQA
                     QSRLKMLERLQAVEPVRARREWRFDFSPPVRTPERLIDAEDLQLGYGERVVLERVKFL
                     VRAGDRIGVLGVNGAGKSTLVKAIAGELAPRSGELRRGQGLAIGYFAQHQLDQLRGDE
                     SPLAHLRRQVPDAREQELRNFLGQFRFSGDMASAPVGPMSGGEKARCALALIAWHRPN
                     LLVLDEPTNHLDMETREALTMALSTFEGALLLVSHDRHLLRAATDQLWLVHDGRLAPF
                     DGDLDDYAALAFASRRPASEARANDEAPTRRELRKAEAQERQRLANARRPLQQRLQKV
                     ETDLAQVSDQLRELDARLAQPDFYHGGDAAEVAAVLKQRGELAQRVEQLESRWLELQS
                     ELEAIT"
     gene            complement(2045..>2485)
                     /locus_tag="AC16HB_16385"
     CDS             complement(2045..>2485)
                     /locus_tag="AC16HB_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HIR5608420.1"
                     /translation="FPNATVDAIDVSTDALAVAQRNVADYGFAKRINLLSSDLFDSAP
                     NKRYDVIVCNPPYVTDASMATLPREYQHEPRLALAGGADGLNIVRRVIRGSRGYLKRG
                     GVLFVEVGDGRPAVEAAFRDLPLTWVTTSAGDDMVFMVRQEDLP"
BASE COUNT          380 a          854 c          852 g          399 t
ORIGIN      
        1 tgcgcatgta gtcgccttcc ccggtgtcct cgaccttgat cacgtcggcg ccgaagtccg
       61 cgaggtgcat cgtcgccgcc ggtcccggca gcaggcgcgt caggtcgagg atgcgcagcc
      121 ccgccaatgg ctgtgtcatg tgatcgcctc cagttccgat tgcagttcga gccagcgcga
      181 ctcgagttgc tcgacgcgct gcgcgagttc gccgcgctgc ttcagcacgg cagcgacttc
      241 ggcggcatcc cctccatggt agaagtccgg ctgcgcaagg cgcgcatcga gctcgcgcaa
      301 ctggtcgctc acctgtgcaa gatcggtctc gaccttctgc agccgttgct gcaacggtct
      361 gcgcgcgttg gcgagccgct ggcgctcctg cgcctcggcc ttgcgcagct cgcgtcgcgt
      421 cggcgcctcg tcgttggcgc gtgcttcgct ggcggggcgc cggctcgcga atgccagcgc
      481 tgcgtaatca tcgagatcgc cgtcgaaagg cgcgaggcgg ccgtcgtgca ccagccacaa
      541 ctgatcggtc gcggcgcgca gcaggtgacg gtcgtgcgac accagcagca gtgcaccttc
      601 gaacgtcgac agcgccatcg tcagcgcctc gcgtgtctcc atgtccagat gattcgtcgg
      661 ttcgtcgagc acgagcagat tggggcggtg ccaggcgatc agcgccagcg cgcagcgcgc
      721 tttctcgccg cccgacatcg gaccgacggg cgccgacgcc atgtcgccgg agaagcgaaa
      781 ctgaccgaga aagttgcgca gctcctgctc gcgcgcatct ggcacctggc gccgcaaatg
      841 cgcgagcggt gattcgtcgc cgcgcagctg gtcgagctgg tgctgcgcga agtagccgat
      901 tgccagtccc tgtccacgcc gcagttcgcc ggagcgcggc gcaagctcgc cagcgatcgc
      961 cttcaccagc gtcgacttgc cggcgccatt gaccccaagg acgccgatgc gatcgccggc
     1021 gcgcacgagg aacttgacgc gctcgagcac cacgcgctcg ccgtagccca actgcaggtc
     1081 ctccgcatcg atgagccgct ccggcgtgcg caccggcggg gagaaatcga aacgccactc
     1141 gcgccgcgcc cgtactggct cgaccgcctg cagccgctcg agcatcttga gccggctctg
     1201 cgcctggcgc gccttggtcg ccttggcgcg gaagcgatca acgtagcttt gcaggtgcgc
     1261 ggcggtgcgc tcgtaagcct tggcggcggc gtcctgctgc ttctgctgtt cgagccatgc
     1321 cagctcgaac gcggtgtagt tgcccgcgta acggcggatc gcgccgtcgg cgacgtgcca
     1381 cgtggtcgtg gtcacgcggt cgaggaattc gcggtcatga gagatcacga gcacggtggc
     1441 gtcctgccgc ttcaaccacg cttcgagcca gacgaccgag tcgaggtcga gatggttggt
     1501 cggctcatcg agcagcagca ggtccgcggg ccgcagcagc gcacgcgcca gcgcgagccg
     1561 gttgcgccag ccgcccgaga aatccgcaac gcggcgcatg ccgtcgcgct cggcgaagcc
     1621 gaggccgtgc atcaccgcct tggcgcgtgc aacgatcgcg ccttcgttca gttcggccag
     1681 ccgtgcgtgc gcatgagcca gccgcaggtc gtcgtggccg gcggtcgcgc gctcgagctc
     1741 gacgcgtgct gcgaccagcg gcgcatgacc ggcgagcacg taatcgagcg cggactcgcc
     1801 ggtggcaccg atgtcctgcg cgacatgcgc gatgcgtgcc ggcggcggcg cttcgagcgt
     1861 gccggcatcg atcgacagtt caccgaggat cgccgcgaac agcgacgact tgccgctgcc
     1921 gttggcgccg acgaggccga cgcgctcgcc gggcggcgcg atcagcgaga cgttgctgta
     1981 aagggttcgg atcccgcgtg cgagcgagac gtcgatcagg cgcagcacgg tctggatgcc
     2041 cggtctacgg caagtcctcc tgccgcacca tgaacaccat gtcgtcgccg gcgctggtgg
     2101 tgacccaggt cagcggcagg tcgcggaacg cggcttcgac ggccggacgg ccgtcgccga
     2161 cctcgacgaa cagcactccg ccgcgcttca gatacccgcg gctgccgcgg atcacgcgcc
     2221 gaacgatgtt cagcccgtcg gcaccgcccg cgagcgcgag tcgtggctcg tgctgatact
     2281 cgcgcggcag cgtcgccatc gacgcgtcgg tcacgtaggg cggattgcag acgatgacgt
     2341 cgtagcgctt gttaggagcc gaatcgaaca ggtcggagct cagcagattg atgcgcttgg
     2401 cgaaaccgta atcggcgacg ttgcgctgcg cgaccgccag cgcatcggtc gatacgtcga
     2461 tcgcatcgac ggtcgcattg ggaaa
//
LOCUS       DBNHBY010000689         2235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1336869, whole genome shotgun sequence.
ACCESSION   DBNHBY010000689 DBNHBY010000000
VERSION     DBNHBY010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1336869"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1010
                     /locus_tag="AC16HB_16390"
     CDS             138..1010
                     /locus_tag="AC16HB_16390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5608421.1"
                     /translation="MNSVIEPGTVAVPASALEACARPLARARIVQALGAAGEPIEVEV
                     VRETGSTNSDLLTQARAQQPARPRLRAALMQSAGRGRHGRRWHSSPGAALLFSLAVPL
                     AEAPHAVSAITLACGLAIAEALAEDGVSAQLKWPNDVLLDGRKLAGILCELAQDGAGR
                     RTLVIGVGMNLWADAAMRASAAQPLATLAERIDPVRLAATREERIASLARALLRLARD
                     CERDGFVPLQPRFMRWFAHTDTEVEIVDRGTRVLHGRALGVDGEGRLLLQTEAGLRAI
                     SSGEMSVRVAPAKP"
     gene            1007..1783
                     /locus_tag="AC16HB_16395"
     CDS             1007..1783
                     /locus_tag="AC16HB_16395"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5608422.1"
                     /translation="MTWLLLDAGNSALKWATAAPQARTLVPGGAVALADLPGALAEAL
                     KLERVSAAFGCNVAGAAIARAIESAVGGACGVPVAWFASRPEFASDGVRLCNRYRDPA
                     QLGADRWHALLAARAAHPGRALVVVNAGTATTVDCISSDGDFVGGVIAPGVQLMFDSL
                     ARRTAQLPLADGTRVAHPDNTDDAITTGVLESQLGLIEHRVRRFAADAGPVLVLLDGG
                     NAAALRAHLSFDARLATVQQESNLVLRGVLIRARALSPGR"
     gene            1787..>2235
                     /locus_tag="AC16HB_16400"
     CDS             1787..>2235
                     /locus_tag="AC16HB_16400"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5608423.1"
                     /translation="MRALLLVLLLANLVLFAYQQGWFGQPTPVGREPGRVALQIEPQA
                     IRRLSEDELRRLRDRTRPAGGNGERRDAAACVDFGDFAADVAVRVQARLDALSPAPRI
                     TTTEVESGGWYMVFVPPQKTRAEAERLADDLRGRGVRDLMVIEDTSSL"
BASE COUNT          287 a          774 c          866 g          308 t
ORIGIN      
        1 tggcgagcgc gcgcgccagc gttgagccgc ggcgcgtccg cacgggctcg aggcgagtgc
       61 tgtcggacac gccgcgattg tcggtgcgcg tgcgcatcac agcaagcgcg ccggccccga
      121 tgctaaagtg gccgcgcatg aattccgtca tcgaaccggg caccgtcgcc gtgccagcga
      181 gcgcgctcga ggcttgtgcg cggccgctcg cgcgcgcgcg catcgtgcag gcgctcggcg
      241 cggccggcga gccgatcgag gtggaggtgg tgcgcgagac cggttcgacc aacagcgacc
      301 tgctgacgca agcgcgtgcg caacaaccag cgcggccgcg gctgcgcgcc gcgctgatgc
      361 aatcggctgg gcgcggccga cacggccgcc gctggcacag cagccccggc gccgcgttgc
      421 tgttctcgct cgcggtgccg ctcgccgaag cgccgcacgc ggtgtccgcg atcacgctcg
      481 cgtgcggctt ggcgattgcc gaggcgctgg ccgaagacgg cgtcagcgcg cagctgaagt
      541 ggccgaacga cgtgctcctc gacggtcgca aactcgccgg catcctgtgc gaactcgcgc
      601 aggacggcgc cggccggcgc acgctggtga tcggcgtggg catgaatctg tgggccgacg
      661 cggcgatgcg agcgtcggcg gcgcagccgc tcgcgacgct ggcggagcgg atcgacccgg
      721 tgcggctggc ggcgacgcgc gaggaacgca tcgcgtcgct ggcgcgcgcg ctgctgcggc
      781 tggcgcggga ctgcgagcgc gacggcttcg taccgttgca gccccgcttc atgcgctggt
      841 tcgcgcacac cgacaccgag gtcgagatcg tcgaccgcgg cacgcgcgtg ctgcacgggc
      901 gagcgctcgg cgtcgacggc gagggccggc tgctgctgca gaccgaagcc gggctgcgcg
      961 cgatttccag cggcgaaatg tcggtgcgcg tcgcaccggc caagccatga cgtggctgct
     1021 gctcgacgcc ggcaactcgg cgttgaagtg ggccacggcc gcgccgcaag cgcgcacgct
     1081 agtgcccggc ggcgcggtgg cacttgccga tctgccgggc gcgctggccg aagcgctgaa
     1141 gctcgagcgg gtgagcgcag cgttcggctg caacgtcgcc ggcgccgcca tcgcgcgcgc
     1201 gatcgagagt gcggtgggcg gcgcgtgcgg cgtgccggtc gcgtggttcg cgtcgcggcc
     1261 ggagttcgcc tctgacggcg tgcggctgtg caaccgctac cgcgatccgg cgcaactcgg
     1321 tgccgaccgc tggcatgcgc tgctggccgc gcgcgcggcg caccccgggc gcgccctggt
     1381 cgtcgtgaac gcgggcacgg caacgaccgt cgactgcatc agcagcgacg gggatttcgt
     1441 cggcggcgtg atcgcgcccg gcgtgcagct gatgttcgac agtctggcgc gacgcaccgc
     1501 gcagttgccg ctcgccgatg gcactcgggt cgctcacccc gacaacaccg acgacgcgat
     1561 caccacgggc gtgctggaga gccagctcgg gctgatcgag caccgcgtgc gccgcttcgc
     1621 ggccgatgca ggtccggtgc tggtgctgct cgacggcggc aatgcggcgg cgctgcgcgc
     1681 gcacctttca ttcgatgcgc ggctggcgac cgtgcaacag gagtcgaacc tcgtgctgcg
     1741 cggcgtgctg atacgcgcgc gagcgctgtc gccggggcgc tgagcgatgc gggcgctgct
     1801 gctggtcctt ttgctcgcga acctcgtgct gttcgcgtat cagcagggct ggttcggcca
     1861 gccgacgccg gtgggccgcg agccgggccg cgtcgcgctg cagatcgagc cgcaggcgat
     1921 ccggcgcttg agcgaagacg agctgcgccg gttgcgcgac cggacccgac cggcgggcgg
     1981 caacggcgag cgccgggatg ccgcggcatg cgtcgacttc ggtgatttcg ctgccgacgt
     2041 ggcagtgcgc gtgcaggcgc ggctcgacgc actcagcccc gcgccgcgca tcacgacgac
     2101 cgaagtcgag agcggcggct ggtacatggt gttcgtaccg ccgcagaaga cgcgggcgga
     2161 ggcggagcgc ctcgccgacg acctgcgcgg ccgcggcgtg cgtgacctga tggtgatcga
     2221 ggacacttcg tcgct
//
LOCUS       DBNHBY010000690         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_592260, whole genome shotgun sequence.
ACCESSION   DBNHBY010000690 DBNHBY010000000
VERSION     DBNHBY010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_592260"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..630
                     /locus_tag="AC16HB_16405"
     CDS             439..630
                     /locus_tag="AC16HB_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR5608424.1"
                     /translation="MTRRRLMFAGAGSAGALAAAASVIPLTKGEPAPDSSAAKAAIES
                     GGGYQVTQHVLRYYETAKV"
     gene            696..>1846
                     /locus_tag="AC16HB_16410"
     CDS             696..>1846
                     /locus_tag="AC16HB_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5608425.1"
                     /translation="MLLTRKTDRVDTATSSLVASLARGVVRAVPTMDRRTFLRRSFLG
                     VGTGLAASQLTLVKKARAADAAPTATGGGKVEVRRTVCGHCSVGCAIDAVVENGIWVR
                     QEPVFDSPINLGAHCAKGAAVREHGHGEFRLKYPMKLVNGKYQRITWDAALDEISAKM
                     LELRKASGPDSVFVVGSSKHSNEQSYLLRKWMSLWGSNNCDHQARICHSTTVAGVANT
                     WGYGAMTNSYNDMQNSKAAMYIGSNAAEAHPVSMLHMLHAKETGTKMIVVDPRFTRTA
                     AKADEFVRIRSGSDIPFLFGVLYHVFKNNWDDKKYIADRVYGMDKVREDVLAKWTPDK
                     VEEACGVDEATCLKVARIMAENRPSTIVWCMGQTQHTIGNAIVRASCIL"
BASE COUNT          361 a          559 c          617 g          309 t
ORIGIN      
        1 cgtcacgcag tgtgcgcgcg gcgttcccga actgtccttc atcgatcatc acgtctgcga
       61 tgcggaagcg ccacggctcc caagggcttg ggtccttgcg gcgttcgatc agcacggcga
      121 cgccgagtga aggtgcggga ttcatgcagg cgcggtccgt aggcttgaag caggggcgcc
      181 aacccattct agtcgcgagc gcgtaacacc aatcggtcgt atcgaagaat cctgtcaggc
      241 gtgatggtca tggaaccgcg gtcgtcggcg acgtattgca gcctgcgccc cttgcgcgag
      301 gcgcgcggga cgactatgtt cgcaatctca taagcagaaa aaatcgaaaa acacaattcg
      361 gaccgcgtga aagaaggagc cgggcgcggc cgtgcagaat ttcggaggca tgtaatgcgc
      421 gaaccttcga ttcgtcccat gacgcggcgt cgcctgatgt ttgccggcgc cggaagcgcc
      481 ggggcgttgg ccgcagcggc cagcgtcatc ccgttgacaa agggcgagcc tgcgcccgat
      541 tcgagcgccg cgaaggcagc gatcgaatca ggtggcggct accaggtcac ccaacacgtt
      601 ctgcgttact acgagaccgc caaggtctga cctgcatgcg tcgccgatcc ggcgcggcgc
      661 agtcttttcg atgacctctc actagagggt attccatgct gctcactcgc aagacagacc
      721 gggtggacac cgccacgtcc agcctggtgg ccagtctcgc gcgcggcgtc gtgcgcgcgg
      781 ttccgacgat ggaccggcgc acgttcctgc gccgatcgtt cctgggcgtc ggcaccggac
      841 tcgcggcttc ccagctgacg ctggtgaaga aggcccgggc ggccgatgcc gcaccgacgg
      901 ccaccggcgg cggcaaggtc gaagtcaggc gcacggtgtg cggtcattgt tccgtgggct
      961 gcgcgatcga tgccgtcgtc gagaacggta tctgggtacg gcaggagccg gtgttcgatt
     1021 cgccgatcaa tctcggcgcc cactgcgcca agggggcggc agtgcgcgag cacggccacg
     1081 gcgagttccg cctgaagtac ccgatgaagc tcgtcaacgg caagtatcag cgcatcacgt
     1141 gggatgcggc gctggacgaa atcagcgcaa agatgctcga gctgcgcaag gcaagcggcc
     1201 ccgattcggt ctttgtcgtc ggctcgtcca agcacagcaa cgagcagtcg tacctgctgc
     1261 gcaagtggat gagcctgtgg ggcagcaaca actgcgacca ccaggcccgt atctgtcatt
     1321 cgaccacggt ggccggcgtg gccaacacgt ggggctacgg tgcaatgacc aattcgtaca
     1381 acgacatgca gaactcgaag gccgcgatgt atatcggcag caacgcggcc gaggcgcacc
     1441 cggtgtcgat gctgcacatg ctgcacgcca aggagaccgg taccaagatg atcgtggtcg
     1501 atccgcgctt cacgcgcacg gcggccaagg cggatgaatt cgtgcggatc cggtctggat
     1561 cggatattcc gttcctgttc ggcgtgctgt accacgtgtt caagaacaac tgggacgaca
     1621 agaagtacat cgcggaccgc gtgtacggca tggacaaggt gcgtgaggac gtcctggcca
     1681 agtggacgcc cgacaaggtg gaggaagcgt gcggcgtgga cgaggcgacc tgtctgaagg
     1741 tcgcccgaat catggccgag aaccgcccga gcacgatcgt gtggtgcatg ggacagacgc
     1801 aacacacgat cggcaacgcg atcgtgcgcg cgtcgtgcat cctgca
//
LOCUS       DBNHBY010000691         1710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_389854, whole genome shotgun sequence.
ACCESSION   DBNHBY010000691 DBNHBY010000000
VERSION     DBNHBY010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_389854"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /gene="mtnA"
                     /locus_tag="AC16HB_16415"
     CDS             <1..1114
                     /gene="mtnA"
                     /locus_tag="AC16HB_16415"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR5608426.1"
                     /translation="GIRTIVRMKVKGVAYRSIWTVTRPGQHRRVAIVDQVRLPYEFAT
                     AELASHDDVADAISSMRVRGAPLIGVAAAYGIALAMQDDPSDGSLESAYRRLLATRPT
                     AVNLRWALDRMCALLAPASEMDRAELAWHEADRIAEEDVELNRAIGTHGARLIADVHA
                     RNGGPVNVLTHCNAGWIATVDYGTALSAIYQAHDAGTPVHVWVDETRPRNQGLLTAWE
                     LAAHGVPHTLIVDNAGGHLMQHGRVDIVLVGADRVTARGDVCNKIGTYLKALAARDCG
                     VPFYAAVPSPTIDWSIDDARADIPIEDRPGAEVHRVRGRAGGDVVEVELAGIETQIAN
                     PAFDVTPARLVTGIITERGLAEPAQLARLFPARGKR"
     gene            1111..>1710
                     /locus_tag="AC16HB_16420"
     CDS             1111..>1710
                     /locus_tag="AC16HB_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR5608427.1"
                     /translation="MTEAEDSRALREALIDTARAMNAAGINVNKSGNVSVRCRRGAAA
                     GFLITPTGIPYAQLAADELVFVRLADAGRVGRREPSSEWRFHYDILRTRGEFNAIVHT
                     HSRAATALSVHGRGIPAFHYMVATAGGNDIRCARYATFGTQALSDHALEALAGRKACL
                     LAHHGVIACEDSLAAALRLAIEVENLAQTYLYARSLGEPP"
BASE COUNT          274 a          589 c          584 g          263 t
ORIGIN      
        1 aggcatccgt acaatcgttc gcatgaaagt caagggcgtc gcctaccgct cgatctggac
       61 cgtgacgcgc ccggggcagc acaggcgtgt cgcgatcgtc gaccaggtac ggctgccgta
      121 cgagttcgcg accgcggaac ttgccagtca cgacgacgtg gcggacgcga tttcctccat
      181 gcgcgtgcga ggcgcgccgt tgatcggggt ggccgccgcg tacggcatcg cgcttgcgat
      241 gcaggacgat ccgtccgacg gtagcctcga gtcggcgtat cggcgcctgc tcgcaacgcg
      301 tccgaccgcc gtcaacctgc gctgggcact ggatcggatg tgcgcgctgc ttgcgcccgc
      361 atccgagatg gaccgcgcgg aactcgcctg gcacgaagcc gaccgcatcg ccgaagagga
      421 cgtcgaattg aaccgcgcca tcggcacgca tggcgcgcgg ctgatcgcgg atgtgcatgc
      481 gcgcaacggc ggcccggtca acgtgctgac ccactgcaat gccggctgga tcgccaccgt
      541 cgactacggc accgcgctgt cggcgatcta tcaggcgcac gacgccggca cgccggtgca
      601 cgtgtgggtc gacgagacgc gccctcgcaa ccagggcctg ctgaccgcgt gggaactggc
      661 ggcgcatggt gtgccccaca cactgatcgt cgacaacgcc ggcggtcatc tgatgcagca
      721 cggccgcgtc gacatcgtgc tggtcggcgc cgaccgcgtc acggcgcgtg gcgatgtgtg
      781 caacaagatc ggcacttacc ttaaagcgct ggccgcccgc gattgcggcg tgccgttcta
      841 cgccgcggta ccgtcgccca cgatcgattg gtcgatcgac gacgcgcgcg cggacatccc
      901 gatcgaggat cgtcccggcg cggaggtgca ccgcgtgcgc ggtcgtgccg gcggcgacgt
      961 ggtcgaagtc gaactggcgg gcatcgaaac gcagatcgcg aatccggcgt tcgacgtcac
     1021 gcccgcacgg ttggtgaccg gcatcattac cgagcgcggc ctcgccgagc cggcccagct
     1081 cgcgcggttg tttccggctc ggggcaagcg atgaccgaag ccgaagacag ccgcgcactg
     1141 agggaagcct tgatcgatac ggcgcgcgcg atgaacgcgg cgggcatcaa cgtcaacaag
     1201 tccggcaacg tatcggtgcg ctgccggcgc ggcgcggcgg cgggcttcct gatcacgccg
     1261 accggcatcc cctatgcgca gctggcagcg gacgaactcg tgttcgtgcg ccttgcagac
     1321 gccggccgcg tcggccgccg agagccaagc agcgagtggc gctttcacta cgacatcctg
     1381 cgcacgcgcg gcgagttcaa cgcaatcgtg catacgcatt cgcgcgcggc caccgcgctg
     1441 tcggtccatg gccgcggcat tcctgcgttt cactacatgg tcgcgacggc gggcggcaac
     1501 gacattcgct gcgcccgcta cgcaactttc ggcacgcagg cgctctcgga ccatgcgctc
     1561 gaggcgctcg ccggacgcaa ggcctgcctg cttgcgcacc acggtgtgat cgcctgcgag
     1621 gattcattgg ccgccgcgct gcggctcgcg atcgaggtcg agaacctggc gcagacctat
     1681 ctgtacgcac gttcgctcgg ggagccgccc
//
LOCUS       DBNHBY010000692        11061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1809958, whole genome shotgun sequence.
ACCESSION   DBNHBY010000692 DBNHBY010000000
VERSION     DBNHBY010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1809958"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..2663)
                     /gene="clpB"
                     /locus_tag="AC16HB_16425"
     CDS             complement(75..2663)
                     /gene="clpB"
                     /locus_tag="AC16HB_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR5608428.1"
                     /translation="MRQDKLTTKFQEALADAQSIAVGNDHQYIEPIHVLAAMLRQSDG
                     AARSLLERAGANVAGMTTAADRALKGLPQVTGTDNQVQVGRDLVGVLNQADKEAQKRG
                     DAYIASEMFLLAVADDKGLAGRTARDHGLTRKSLEAAIDAVRGGQNVGSAEAEGQREA
                     LKKYTIDLTERAQQGKLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNDEVPETLKGKRVLTLDMAGLLAGAKYRGEFEERLKAVLKEIAAEEGRIIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVESTIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARIKMEIDSKPEAMDKLDRRLIQLKIEREAVKKETDEASRKRLGLIEEEIRK
                     LEREYADLDEVLRGEKAAVQGSAHIKAEIDKLKAQMTELQRKGNYEELAKIQYEQLPK
                     LEAQLKSAEKGGKGGKKEFKLLRKEVGAEEIAEVVSRATGIPVSKMMQGEREKLLKME
                     ERLHSRVVGQDEAVRLVSEAIRRSRAGLADPNRPLGSFLFLVPTGVGKTELCKALAEF
                     LFDSEEHLVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEQVRRKPYSVILLDEV
                     EKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVLVMTSNLGSHEIQRMAGQDYDLIK
                     EAVMGEVRRSFRPEFVNRIDEIVVFHALDAKHIASIAKIQLKRLEQRLAAQDMKLEVS
                     DAALKEIAKVGFDAVYGARPLKRAIQQQIENPIAKLVLEGKVGPKDVVPVDFRDGKFT
                     FNRTVH"
     gene            2879..4630
                     /locus_tag="AC16HB_16430"
     CDS             2879..4630
                     /locus_tag="AC16HB_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0097347 - TAM protein secretion complex
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR5608429.1"
                     /translation="MRLYVAALLLLFALAAAGAERPLARFQVAGEPPATLAPLLQKVL
                     EGAGPVETFDVEDDERLLRRLRANALEVLATEGFFSPGIGAEPDTTDAARYVLKVDLG
                     QRARVAGVEIKLTGAIEREPERIRQLTASWDLGIDHPFRDAEWAAAKTRLLNRVRERD
                     YAAARIADSMAEVDADNATVRLHVEIDSGPAFTLGHLEVKGLKRYEENLIERYYPFTS
                     GERYDSAKLLDFQRRLQQAPYFGSVIVDIETSGPHENVPVRVEVTEAKTKRVAFGVGY
                     STNTGPRAEVTYRQALLFGHPYTLVSGAGIDRTRSVAFADILLPPRPSGANDSVGTLF
                     EHTDIENVLTNRWAVGVARAQSRSTDTATYDTQIALSYQSERRRISGSSEPAQTNDVV
                     AATYTWTRRTVDQITDPKRGALMTLSTTAGVRRGSFGDLLQQTFVRLYGRYVRYLPLS
                     PRDQVILRGEAGHVVVDDPALVPNEFLFRAGGVGSVRGYAYQSLGVRKGTATTGSREL
                     LVGSAEYVRWLTETWGAAVFYDIGDADDDLRKVRLARGYGVGARWRTLAGPLAFDIAY
                     GERDQRWRVHFFIAIAF"
     gene            4632..8576
                     /locus_tag="AC16HB_16435"
     CDS             4632..8576
                     /locus_tag="AC16HB_16435"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR5608430.1"
                     /translation="MNAPAAEPPIKRSARWRLFALALLLVVAAAAASFAWLLTTTAGI
                     GTVIAIVNRLVPARIEATGAQGALVRDFGFERLRVVVGGTEVDIRDLRARLRAHALRP
                     PRFDFESLAASRVDVHVTPSGKRSGPPDAIASPVVVTTQRLAVGEFNLAVGTPVLALR
                     TIDAAVALGPDGYRVTGGRFEAGAQQFTLDGELGGSKPFAFVAHGSATATLQTQPVQA
                     QWRTTGSLVDFTLAADVSGGDARGTAQARIASFDEPALKSLQADVDGIDLRAWAAQLP
                     TTRLKIQADLRPDEKLLELSGPVRIANRDAGTIDAGRLPLRAAQGEIVLTRQELRASR
                     VALELPRGRASGTLGVQFESRAWQSDLRLDGVDPAAIHARAKPLLLDGALRAWRDGAA
                     TLVRADLRNRDKPIVNAILDLSVTADRVDVRHADLTLGGGRASVTGVIGLTGEGRVSA
                     KGSIDRIDPGLLVAGVDALLSGDFEADARLRPQPSGQVRFELRDSRAFGRPLTGRGRV
                     QLDAAQQLDVDAQLAVRSARVSATGGLGAPGRTLALALDAPALEELLPALKGSVKAEA
                     TLSGAWNAPAVDARAVASNLRLGDHTVQQAQAVATYSGGTDGRLSVQAGAANHRFRDS
                     AALSLRSATLVIEGPLSQHAISLRGTTNTTQGAALVAQGGWSQHAWRGTLREATVGAP
                     LDARLLAPGPLMASADGFDLGPLQFALAGARFDDVRIAASGASLRTSGSFSGLHPTEL
                     VAPDPASKAVTLRSATPLDLLTLRGQWQLQLGAQADGMLTIERSSGDVYAGASADSGL
                     RLREVRLDARLAANRLEARAAIAGDRAGALGAQMSAYVERAPDAGWRLAQQRPWQIDA
                     NLDMPSLAWVNAVLGERVRANVRLDGRLAGQFSIGGTPAEPQARGALNGDALRLAWVE
                     QGMRLENGRLRARLEGDVVVLDELRFAGPPHARPDDKRAAARLDFTKEGSVNATGQLR
                     VRDLDGVIQVAAERLPLLQRPDRRIIASGGANIEMSLQRVQLNGAFAATAGYVDFSRA
                     ELPSLSSDVIVTRARDEPAARTPRVALGFDVGIDLGDAFYVRGSGLDTRVDGAVRLRS
                     AGRGAVTASGSLEAQEGVYEGFGQRLTIARGRLNFQGPPENPGLDVLAIRRGLPVEVG
                     VTITRTAANPLIRLYSDPPMSDYEALSWLVLGRPAEQASRTDNLALAQAATSLLSGKG
                     QGIPGQLARALGIDEISLRAADARDAGSLLPRRSVAGSLRGDTSIAPTATTEIITIGK
                     RVGEAITVSYEQALAGTASIVQLSYRVSQRLSLVARAGTENALDLVYSIAFD"
     gene            complement(8590..10464)
                     /locus_tag="AC16HB_16440"
     CDS             complement(8590..10464)
                     /locus_tag="AC16HB_16440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR5608431.1"
                     /translation="MKCRTIFILLGALAAAAPAFAQTMAEPPAAGLVQRAIGAGRNDP
                     EDARRLAEEALALIAAKPDADLEVRALLVLCDFWSERKLDTARDVLARATALLSVAKR
                     GGLRAGVQSCEGEIEESAGDYSKAMAAYARAVSSAEVASDEEMLANALYQRGWLRGVQ
                     GDYALGLIDLRRAVTLFEKANMAEHARTAVNGVATIYNRMGDYAQAQHFYGLALKSQL
                     AMGTQREAVVTLFNLGRTRENLNDWDGARKDYLHALEISTRLGYARGEAYALRGLAGV
                     HNARGEWGSALDQLAAAQKLATAVPDARLQAQIALLRGVTLRGLKRTGESVASLNAAL
                     ASFRAAEALAEQAATLAALAASLADMGDWRAAYEAQRQLKEVTDRVHARQLDQRFTSL
                     KVEFDTAAREKENALLVREKAAIEAALEQQKRIGALQVVVIMLAAVLAVLLGLLAWRH
                     RQASRRMQALALTDELTGLPNRRAVLARLEEMLKSQHACAALIIDLDHFKVINDRHGH
                     LVGDEVLRAVTAMLIDVVRAPMFVGRMGGEEFLLLLPDASAATARQVAERVRGAVAGL
                     DASSWPGGCALSVSIGMTIARPGADAVSDVLRRADEALYAAKAAGRNRVHESTLTVAA
                     "
     gene            complement(10594..10908)
                     /locus_tag="AC16HB_16445"
                     /pseudo
     CDS             complement(10594..10908)
                     /locus_tag="AC16HB_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1601 a         3923 c         3752 g         1785 t
ORIGIN      
        1 tgagcggctc ccgttgaacc gcaagtcgcg tgggggcgtg ccgtgccgac gcgcgcctgg
       61 cacgtcgtgc cttgctaatg caccgtccga ttgaacgtga acttcccgtc gcggaaatcc
      121 accggcacca cgtctttcgg cccgaccttt ccttcgagca cgagcttcgc gatagggttc
      181 tcgatctgct gctggatcgc gcgcttcagc gggcgcgcac cgtagacggc gtcgaagccg
      241 accttggcga tttccttcag cgcggcatcc gatacctcga gcttcatgtc ctgcgcagcg
      301 agccgctgct ccaggcgctt gagctggatc ttcgcgatcg acgcgatgtg cttggcgtcg
      361 agcgcatgga acacgacgat ctcgtcgatg cggttgacga actcggggcg gaagctcctg
      421 cgcacctcgc ccatcaccgc ctccttgatc aggtcgtagt cctgcccggc catgcgctgg
      481 atctcgtgcg agccgaggtt gctggtcatc accagcacgg tgttcttgaa gtcgaccgtg
      541 cggccctgcc cgtcggtcat gcggccgtcg tcgagcacct gcagcagcac gttgaacacg
      601 tccgggtgcg ccttctcgac ttcgtcgagc aggatcacgc tgtacggctt gcgacgcacc
      661 tgctcggtga gctggccgcc ttcctcgtag ccgacgtagc ccggtggtgc gccgatgagc
      721 cgcgcgaccg agtgcttctc catgtactcg ctcatgtcga tgcggaccag atgctcctcg
      781 ctgtcgaaca ggaactcggc cagcgccttg cacagctctg tcttgcccac gcccgtaggc
      841 acgagaaaga ggaacgaccc cagcggccgg ttcggatccg ccagccccgc gcgcgaacga
      901 cggatcgctt cggacaccag ccgcacggct tcgtcctggc ccaccactcg cgagtgcagc
      961 cgctcttcca tcttcagcag cttctcgcgc tcgccctgca tcatcttgct gacggggatg
     1021 ccggtggcgc gcgagacaac ctcggcgatc tcctcggctc cgacttcctt gcgcagcagc
     1081 ttgaattcct tcttgccgcc cttgccgccc ttttcggccg atttcagctg cgcttcgagc
     1141 ttgggcaact gctcgtactg gatcttggcg agctcctcgt aattaccctt gcgctgcagt
     1201 tcggtcatct gcgccttgag cttgtcgatc tcggccttga tgtgcgcgct gccctgcacc
     1261 gcggctttct ccccgcgcag cacttcgtcg aggtccgcgt actcgcgctc gagcttcctg
     1321 atctcctcct cgatcaggcc gagccgcttg cgcgaggcct cgtcggtttc cttcttcacc
     1381 gcctcgcgct cgatcttcag ctggatcagc cggcggtcga gcttgtccat cgcctccggc
     1441 ttggagtcga tctccatctt gatccgcgct gcggcctcgt cgatcaggtc gatcgccttg
     1501 tcgggcagga agcggtcggt gatgtagcgg tgcgacagct ccgccgcggc gacgatggcc
     1561 gggtcggtga tctcgacacc gtggtgcagc tcgtacttct cctgcaggcc gcgcaggatg
     1621 gcgatcgtgc tttcgacgct cggctcgtcg acgagcacct tctggaagcg gcgttcgagc
     1681 gcggcgtcct tctcgatgta tttccgatac tcgtcgagcg tggtcgcgcc gacacaatgc
     1741 agttcgccgc gcgccagtgc gggcttcagc atgttgccgg cgtcgatcgc gccctcggcc
     1801 ttgccagcac cgaccatcgt gtgcaactcg tcgatgaaga cgatgatcct gccttcctcg
     1861 gcggcgatct ccttcaacac ggctttcagc cgttcctcga attcgccgcg gtacttcgcg
     1921 cccgcgagca gtcccgccat gtcgagcgtc agaacgcgct tgcccttgag cgtttccggc
     1981 acttcgtcgt tgacgatccg ctgcgcgagt ccctcgacga tggcggtctt gcccacgccc
     2041 ggctcgccga tcagcaccgg attgttcttg gtgcgccgtt gcaggatctg gatcacgcgg
     2101 cggatctcgt cgtcgcgtcc gatcaccggg tcgagcttgc cctgctgcgc gcgttccgtc
     2161 aggtcgatcg tgtatttctt cagcgcttcg cgctggccct ccgcttccgc gctgccgacg
     2221 ttctggccgc cgcgcaccgc gtcgattgcg gcttcaaggc tctttcgcgt cagcccgtgg
     2281 tcgcgcgcag tgcgacccgc gagccctttg tcgtcggcca ccgcgagcag gaacatttcg
     2341 ctcgcgatgt aggcgtcacc gcgcttctgc gcttccttgt ccgcctggtt cagcacgccc
     2401 accaggtcgc gcccgacctg cacctggttg tcggtgccgg tgacctgcgg caggcctttc
     2461 agcgcccggt cggcggccgt ggtcatgccc gcgacgttcg cgcccgcgcg ctccagcagc
     2521 gaacgtgccg cgccatcgct ctgccgcagc atcgcggcca gcacatggat cggctcgatg
     2581 tactggtggt cgttgccgac cgcgatcgac tgtgcgtcgg ccagtgcctc ctgaaacttg
     2641 gtcgtcagct tgtcctgtcg cattcctcgc cccgaaacgg tggttcctgt tgcgacttgg
     2701 ggccggggac ggaaatttca atcggccgat agaatcgggc gccgttcgac gcggcgttgg
     2761 ttttcgctgg gccctcaccc cgactcctct ctccgaggca acggggcttc ccctactcta
     2821 cgttcgggag cgagggcgag ggaaacgacg gtctcatcga ctgctgccag aacccctcgt
     2881 gcgcctatac gtcgctgccc tcctcctgct gttcgccctg gccgccgccg gcgcggaacg
     2941 gccgctggcg cggttccagg tcgccggaga gccgccggcg acgctggcgc cgctgcttca
     3001 gaaggtgctc gaaggcgccg gtccggtcga aacgttcgac gtcgaagacg acgagcgcct
     3061 gctgcgccgc ctgcgcgcca acgcactcga agtactggcg accgagggct tcttctcgcc
     3121 aggcatcggc gccgaacccg acacgaccga cgccgcgcgc tacgtgctca aggtcgacct
     3181 cggccagcgc gcgcgcgtgg ccggcgtcga gatcaagctc accggcgcga tcgagcgcga
     3241 gcccgagcgg atccgccaac tgacagcgag ttgggacctg ggcatcgacc atccgtttcg
     3301 cgacgccgag tgggcagccg ccaagacacg gctgctgaac cgcgtgcgcg agcgcgacta
     3361 tgccgccgcg cgcattgcag attcgatggc cgaggtcgac gccgacaatg cgacggtgcg
     3421 gctgcacgtc gagatcgaca gcgggcccgc attcacgctc ggccacctcg aggtcaaggg
     3481 actcaaacgc tacgaagaga acctgatcga gcgctactac ccgttcacgt cgggcgaacg
     3541 ctacgactcc gccaagctgc tcgacttcca gcgccggctg cagcaagcgc cctacttcgg
     3601 ctcggtcatc gtcgacatcg agaccagcgg accgcacgag aacgtgccgg tgcgcgtcga
     3661 agtgactgag gccaagacca agcgcgtcgc gttcggcgtc ggctactcga ccaacaccgg
     3721 cccgcgcgcg gaagtgacat accggcaggc gctgctgttc ggccacccgt acacgctcgt
     3781 cagcggcgcc ggcatcgacc gcacgcgcag cgtggcgttc gccgacatcc tgctgccacc
     3841 cagacccagt ggcgccaacg acagcgtcgg cacgctgttc gagcacaccg acatcgagaa
     3901 cgtgctgacc aaccgctggg cggtcggggt ggcgcgcgcg cagtcacgca gcaccgatac
     3961 cgcaacctac gacacgcaga tcgcgctcag ctaccagagc gagcggcgcc gcatctccgg
     4021 gagcagcgaa cctgcgcaga ccaacgacgt ggtcgcggcg acctacacgt ggacgcgccg
     4081 cacggtcgac cagatcaccg acccgaagcg cggcgctttg atgacgctgt cgaccaccgc
     4141 cggcgtgcgc cgcggctcgt tcggcgacct gctgcagcag actttcgtgc gtctgtacgg
     4201 ccgctacgtg cgctacctgc cgctgtcgcc gcgcgaccag gtgatcctgc ggggcgaagc
     4261 gggccacgtg gtggtcgacg accccgcgct ggtgccgaac gagttcctgt tccgtgccgg
     4321 tggcgtcggc agtgtgcgcg gctatgccta ccagagcctg ggcgtgcgca agggcacagc
     4381 caccaccggg agccgcgagc tgctggtggg atcggccgag tacgtgcgct ggctgaccga
     4441 gacctggggc gccgccgtct tctacgacat tggcgacgcc gacgacgatc tgcgcaaggt
     4501 gcgcctggcg cgcggttacg gcgtcggcgc ccgttggcgc accctggccg gaccgctcgc
     4561 gttcgacatc gcctacggcg agcgcgacca gcgctggcgg gtgcacttct tcatcgcaat
     4621 tgcgttctga gatgaacgcc cctgcggccg aaccgccgat caagcgctcc gcgcgctggc
     4681 gtcttttcgc gctggcgctg ttgctcgtcg tcgccgcagc ggcggcgtcg ttcgcctggc
     4741 tgctgacgac gaccgccggc atcggcacgg tgatcgcgat cgtgaatcgg ctggtgccgg
     4801 cgcgcatcga ggcgaccggt gcgcagggcg cgctggtgcg cgacttcggt ttcgagcgtc
     4861 tgcgcgtggt cgtcggcggc accgaggtcg atatccgcga cctgcgtgcg cgcctgcgcg
     4921 cccacgcgct gcgcccgccg cgcttcgact tcgaatcgct cgcggcttcc cgcgtcgacg
     4981 tgcacgtcac gccgagcggc aagcggtcgg gcccgccgga tgcgatcgca tcgcctgtcg
     5041 tcgtgacgac gcagcggctg gccgtcggcg aattcaatct cgccgtcggc acgcccgtac
     5101 tcgcgctgcg cacgatcgac gccgcggtcg cgctcggccc ggatggctat cgcgtgaccg
     5161 gcggacgctt cgaggcaggc gcgcagcagt tcacgctcga cggcgaactg ggcggcagca
     5221 agccgttcgc tttcgtcgcg cacggcagtg caacggccac cctgcagacg cagcccgtgc
     5281 aggcgcagtg gcgcactaca ggatcgctgg tcgacttcac gctcgccgcc gacgtctccg
     5341 gcggtgatgc gcgcggcact gcgcaggcgc gcatcgcgag cttcgacgag ccggcgctca
     5401 agtcgctgca ggccgacgtc gacggcatcg atttgcgtgc gtgggccgcg cagttgccaa
     5461 ccacgcggct caagattcag gccgacctgc gtcccgatga aaagctgctc gagctcagtg
     5521 ggccggtgcg tatcgcgaat cgcgacgccg gcacgatcga cgctggcagg ctgccgctgc
     5581 gcgccgcgca gggcgagatc gtgctgacgc ggcaggaact gcgcgcgagc agagtggcgc
     5641 tggaacttcc gcgcggtcgt gcgagcggga cgctcggcgt gcagttcgaa agtcgcgcgt
     5701 ggcagtccga cctgaggctc gacggcgtcg atccggcggc gatccacgcg cgcgcaaagc
     5761 cgctgctgct cgacggcgcg ttgcgcgcat ggcgcgacgg cgcggcgacg cttgtgcggg
     5821 ccgatctgcg caatcgcgac aagccgatcg tcaatgccat tctcgatctg agcgtcacgg
     5881 ccgatcgcgt cgacgtaagg cacgctgacc tgacgcttgg cggcggccgt gcgagcgtga
     5941 cgggcgtgat tggcctgacc ggtgagggac gagtcagcgc gaaaggctcg atcgatcgca
     6001 tcgatcccgg cctgctggtc gcgggcgtcg acgcgctgct cagcggcgac ttcgaggccg
     6061 atgcgcgact gcggccgcag ccgagcggtc aggtgcgctt cgagctgcgc gacagccgcg
     6121 cgttcggacg gccgctgacg gggcgcgggc gcgtgcagct cgacgccgcg cagcaactcg
     6181 acgtcgacgc gcagctcgca gtgcgctcgg cgcgcgtgag cgcgaccggc gggctcggcg
     6241 caccaggccg cacgctcgcg ctggcgctcg acgcgcccgc actggaggag ttgctgcccg
     6301 cgctgaaagg gtccgtgaag gcggaggcaa cgctgagcgg cgcgtggaat gcgccggcgg
     6361 tcgacgcgcg cgccgtcgcg agcaacctgc gcctgggtga tcacacggtg cagcaggcgc
     6421 aggcggtggc gacctacagc ggcggcaccg acggtcgcct gtcggtgcag gccggtgcgg
     6481 ccaaccaccg ctttcgcgac agcgcggcac tgtcgctgcg cagcgccacg ctggtcatcg
     6541 aaggaccgct gtcgcagcac gcgatctcgc tgcgcggcac gaccaacacc acgcagggcg
     6601 ccgccctggt cgcgcaggga ggctggagcc agcacgcatg gcgcggcacg ctgcgcgaag
     6661 cgaccgtcgg cgcgccgctc gatgcgcggc tgctcgcgcc gggtcccctc atggcgagcg
     6721 ccgacggctt cgatctcgga ccactgcagt tcgcgctcgc gggcgcgcgc ttcgacgacg
     6781 tgcggatcgc ggcgagcggc gccagcctgc gcaccagcgg cagcttcagc gggctgcacc
     6841 cgaccgagct cgtcgcgccc gatccagcca gcaaggcagt gacgctgcgc tcggccacgc
     6901 cgctcgacct gctgacgctg cgcgggcaat ggcaattgca gctcggcgcg caagccgacg
     6961 gcatgctgac gatcgagagg agcagcggcg acgtttacgc cggcgcgagc gccgacagcg
     7021 gcttgcgcct gcgcgaggtg cggctcgacg cgcggctcgc ggcgaaccgg ctcgaggcgc
     7081 gcgcggcgat cgccggcgat cgcgccggtg ccctgggcgc gcagatgagt gcgtacgtcg
     7141 aacgcgcacc cgacgccggc tggcgcctcg cgcagcagcg gccgtggcag atcgacgcca
     7201 atctcgatat gccgtcgctg gcatgggtca acgcggtcct cggggaacgc gtgcgggcaa
     7261 acgttcggct ggacgggcgg ctggcgggcc agttcagcat tggcggcacg ccggccgagc
     7321 cgcaggcccg cggcgcactc aacggcgacg cgctgcggct ggcgtgggtc gagcagggca
     7381 tgcggctgga gaacggccgg ctgcgcgcgc ggctcgaagg cgacgtggtc gtgctcgacg
     7441 aactgcgctt tgccggcccg ccgcatgcgc ggcccgacga caaacgcgcc gcggcgcggc
     7501 tcgacttcac gaaggagggt tcggtcaatg cgactgggca actgcgcgta cgtgatctcg
     7561 acggcgtgat ccaggtcgcc gccgagcgct tgccgctgct gcagcggccg gatcgccgga
     7621 tcatcgccag cggtggcgca aacatcgaga tgtcgctgca gcgggtgcag ctgaacggcg
     7681 cgttcgccgc caccgcgggc tacgtcgatt tctcacgcgc ggaattgcct tcgctgtcga
     7741 gcgacgtgat cgtcacccgc gcgcgcgacg agccggctgc gcgcacgccg cgcgtggcgc
     7801 tgggcttcga tgtcggcatc gacctgggcg acgcgttcta cgtgcgcggc agcgggctgg
     7861 acacgcgcgt cgacggcgcg gtgcgcctgc gcagcgcggg ccgcggcgcg gtgacggcat
     7921 cgggttcgct cgaagcgcag gaaggcgttt acgaagggtt tggccagcga ctcacgatcg
     7981 cgcgcggacg cctcaacttc caggggccgc cggagaatcc ggggctcgat gtgctcgcca
     8041 tccggcgcgg cctgccggtc gaggtcggcg tgacgatcac acgcaccgcg gccaatccgc
     8101 tgatccgcct gtactccgat ccgccgatgt cggactacga ggcactttcg tggctcgtcc
     8161 tggggcgccc cgccgagcag gcatcgcgca ccgacaacct ggcgctggcg caggccgcga
     8221 ccagtctgct gtccggcaag ggacagggta ttccgggaca actcgcgcgc gcgctcggca
     8281 tcgacgaaat ctcgctgcgc gcggccgatg cacgagatgc gggctcgctg ctgccgcgcc
     8341 gttcggtcgc agggtcgctg cggggcgata cgtcgatcgc gccgactgcc acgaccgaga
     8401 tcatcacgat cggcaagcgc gtcggcgagg cgatcacggt gagctacgaa caggcgctgg
     8461 ccggcacagc cagcatcgtg caactgagct atcgggtgtc gcagcgcctg tcgctggtcg
     8521 cacgcgcggg caccgagaac gcgctcgatc tcgtctattc gatcgcgttc gactgaccct
     8581 cgcggccggc taggcagcca ccgtcaacgt gctctcgtgt acgcggttac gcccggcggc
     8641 cttcgccgcg tagagcgctt cgtcggcacg acgcagcacg tcggagaccg catcggcacc
     8701 cggccgcgcg atcgtcatgc cgatgctgac cgaaagcgca cagccgccgg gccagctcga
     8761 cgcatcgagg ccggccactg cgccacgcac gcgctcggcg acctgccgcg cggtcgccgc
     8821 ggatgcatcg ggcagcagca gcaggaactc ctccccgccc atgcgaccca cgaacatcgg
     8881 cgcgcgcacg acgtcgatca gcatcgcggt gacggcgcgc aggacctcgt cgcccacgag
     8941 gtgcccgtgg cgatcgttga tgaccttgaa atgatcgagg tcgatgatca acgccgcgca
     9001 cgcatgctgt gacttgagca tttcctccag ccgcgccagc actgcgcggc ggttcggcag
     9061 cccggtcagc tcgtcggtca gcgcaagcgc ctgcatgcgg cggctggcct ggcggtgccg
     9121 ccacgcgagc agcccgagca gtacggcgag caccgccgcc agcatgatca cgacgacctg
     9181 caatgcgccg atgcgcttct gctgttcgag cgccgcttct atcgcggcct tctcgcgcac
     9241 cagcagcgcg ttctccttct cgcgcgccgc ggtgtcgaac tcgaccttca gtgacgtaaa
     9301 gcgctggtcg agctggcgcg cgtgtacgcg gtcggtgact tccttcagct gccgttgcgc
     9361 ctcgtaggcg gcgcgccagt cgcccatgtc ggccagcgat gcggccagtg cggcgagcgt
     9421 ggcggcctgc tcggcaagcg cctccgcggc gcggaaagac gccagcgcgg cattgagcga
     9481 tgcgacgctc tcgcccgtcc gcttcagtcc gcgcaatgtc acgccgcgca gcagtgcaat
     9541 ctgcgcctgc aggcgcgcgt cgggcactgc ggtggccagc ttctgcgccg cggccagctg
     9601 atcgagggcg ctgccccact ccccgcgcgc gttgtggacg ccggccagtc cgcgcagtgc
     9661 gtacgcctcg ccgcgcgcgt aaccaagccg ggtgctgatc tcgagtgcat gcaggtaatc
     9721 cttgcgcgct ccatcccagt cgttgaggtt ctcgcgcgtg cggccaagat tgaacagcgt
     9781 gaccacggcc tcgcgctgcg tgcccatcgc gagctgcgat ttcagggcca gcccgtagaa
     9841 gtgctgtgcc tgcgcatagt cgcccatgcg gttgtagatc gtcgccacgc cgttgacggc
     9901 ggtgcgcgcg tgctcggcca tgttggcttt ttcgaacaga gtgaccgcgc gtcgcaggtc
     9961 gatcagcccg agcgcgtagt cgccctgcac gccgcgcagc cagccgcgct ggtacagcgc
    10021 gttggcgagc atctcctcgt cgctggcgac ctcggcgctc gacaccgcgc gtgcgtacgc
    10081 ggccatcgcc tttgaatagt cgcccgcgga ctcctcgatc tcgccctcac agctctgcac
    10141 accagcgcgc agcccgccgc gcttggcgac tgacaacagc gcggtggcgc gcgccagcac
    10201 atcacgcgcc gtgtcgagct tgcgttcgga ccagaagtcg cacaagacca gcagcgcccg
    10261 gacttcgaga tcggcatccg gtttcgcggc gatcagcgcc agcgcctctt ccgcgagccg
    10321 gcgggcatcc tcgggatcgt tgcgtccggc gccgatcgcg cgttgcacga ggccggcagc
    10381 cggaggctct gccatcgtct gtgcgaatgc aggcgcggcg gccgcgagcg cacccagcag
    10441 gatgaagatg gtccggcatt tcatgcggag cgcattctcc cgcgcaatcg gcagcctggg
    10501 atcaaccgca tggccgattc gcccgtgccg cagcgccaac gcaacagcgc gagccgcgga
    10561 tctcacgcgc cgcatgtgga tctgcgctcg aggtcaggac gccggcactt cccgcatcac
    10621 ccggaaccct gcggcgtaca gcgccggcag aaagagcagc gtcagcacgg tcgcgacgac
    10681 cgggccgtcc atgatcgcga ccgccatcgg accccaggac acgctgcgcg acagcaggat
    10741 catcgccagc acggcggcgg ccgtcagaat gatcggacga aggcgacgca cggcggcccc
    10801 gatgatcgcg ttccgggtat cgacgccgcg cgcctcatcg cgctcgatcc ggtcgaccag
    10861 gatgatcgcg ttgcggacga tcatcccgac cagcgcaatc acgccgagcg cgccaccagt
    10921 tgcgctcgcg ctgccaaggc cgcgaggcgg acactggccg ggccgatcgt ggccaggacc
    10981 cgccaggcct tcacggtccg ggcgacttcg accgcgcgca tgacgatgtg gccgccgacc
    11041 aggaagccga cgcgcgcggc g
//
LOCUS       DBNHBY010000693         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_931274, whole genome shotgun sequence.
ACCESSION   DBNHBY010000693 DBNHBY010000000
VERSION     DBNHBY010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_931274"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16HB_16450"
     CDS             complement(<1..251)
                     /locus_tag="AC16HB_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HIR5608432.1"
                     /translation="MPMESAHRLLPGLRQMVADAGGLYLANAVIAFVFAASGPVAIIL
                     AVGARGGLSESDLSSWIFGSFFINGLITLLFCTLYRQPLV"
     gene            529..1317
                     /locus_tag="AC16HB_16455"
     CDS             529..1317
                     /locus_tag="AC16HB_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR5608433.1"
                     /translation="MPMQTVHIERDGPVWLVTLQRPLVRNAVDGPTARALAAAFRDFD
                     AEAAASVAILAGAGGTFCAGADLKAVAEGGARMNPLESDIDADGPMGPTRLRLTKPVI
                     AAVSGHAVAGGLELALWCDLRVIDDTAVFGVFCRRWGVPLIDGGTVRLPRLIGESRAL
                     DLILTGRAVDADEAMTIGLANRRAAPGQSALEAALELAKALAGLPQTCLRSDLMSVKM
                     QAGLPLRAALAQEFALGMATLQSGESRQGAGRFLAGAGRGGRTA"
     gene            1385..>1653
                     /locus_tag="AC16HB_16460"
     CDS             1385..>1653
                     /locus_tag="AC16HB_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608434.1"
                     /translation="MHASEEEAFPAPAFAFQRQPVRALQGPLDLAEGAVVHALRAGDC
                     LELGAGGQRVPQFGAACVPPSGVDAALGRRGVVAFIVRCAAVDFSG"
BASE COUNT          263 a          518 c          612 g          260 t
ORIGIN      
        1 acgagcggct gccggtacag cgtgcaaaag agcagcgtga tcagcccgtt gatgaagaac
       61 gagccgaaga tccacgacga cagatcggac tcggagagcc caccacgcgc gccgacggcg
      121 aggatgatcg cgaccggccc cgaggcggcg aagacgaagg cgatcaccgc gttggcgagg
      181 tagagcccgc cggcatcggc gaccatctgg cgcagtccgg ggagcagtct gtgggcggac
      241 tccatgggca tggcgggctt cggcgatgac gcgcgccgat tgtgccggaa tcacgttcgg
      301 ccacggggcg cgcccgaatc gggcgaaagc ggttggccgg tcggcgcgcc tctcaattcg
      361 tgcacgcgaa tgactgctgt cgcggcctgc gcgaacttcg cttggatgca gtcactccga
      421 gaggcgctcg gcacatggag gaactatgct gaaccgcaga tcgcgcgctt gaatcgaaag
      481 cgtccgtgca acgcgcgcca atgatcccgc acgccaccca ggaggggcat tccgatgcag
      541 acggtgcata ttgaacgcga cggaccggtg tggctggtca ccctgcaacg acctttggtg
      601 cgcaacgcgg tcgacggccc gacggcgcgc gcgttggctg ccgcatttcg tgactttgat
      661 gcagaggcgg ccgcgagcgt cgcgatactc gccggcgccg gcggcacgtt ctgtgccggc
      721 gccgacctga aggcggtggc cgagggcggc gcgcggatga acccgctcga aagcgacatc
      781 gatgctgacg ggccgatggg gccgacgagg cttcggctga ccaagccggt gattgctgcg
      841 gtcagcggtc acgcggtcgc gggtgggctc gagcttgcgc tgtggtgcga cctgcgtgtg
      901 atagacgata ccgctgtgtt cggcgtcttc tgccgccgct ggggcgtgcc gctcatcgac
      961 ggcggcacgg tgcggctgcc gcggctgatc ggcgagagcc gtgcgctcga tttgatcctg
     1021 actgggcgtg cggtcgacgc cgacgaggca atgacgatcg gactggccaa ccggcgcgct
     1081 gcgccggggc agtcggcgct cgaggccgcg ctcgaactgg cgaaagcgct ggcaggactg
     1141 ccgcagacct gtctgcgctc cgacctgatg agcgtcaaga tgcaagccgg attgccgctg
     1201 cgcgctgcgc tggcgcagga gttcgcgctc ggcatggcaa ccctgcagtc gggcgagagt
     1261 cgacaaggcg caggacgatt cctcgcgggc gccggccgcg gagggcgaac ggcctgagcc
     1321 gttcgtcggc cgtcagctct aacgcggttg tcgtcgacct gcgttcaaac tggtcgaagt
     1381 cgaattgcac gcgagcgaag aagaggcctt cccggcgccg gcgttcgcct tccaacgtca
     1441 gccggtacgg gcgctgcagg gaccgctgga tctcgccgaa ggcgccgtgg tgcacgcgct
     1501 gcgggccggc gattgcctcg aactcggcgc cggcggacag cgtgttccgc aattcggcgc
     1561 agcgtgcgtg ccgccatctg gcgttgacgc ggcgctaggg agacgagggg tcgtcgcctt
     1621 tatcgtccgt tgcgccgcgg ttgatttctc tgg
//
LOCUS       DBNHBY010000694         7235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1201391, whole genome shotgun sequence.
ACCESSION   DBNHBY010000694 DBNHBY010000000
VERSION     DBNHBY010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1201391"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..1112)
                     /locus_tag="AC16HB_16465"
     CDS             complement(417..1112)
                     /locus_tag="AC16HB_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5608435.1"
                     /translation="MHTHSFGASLQAPAPDPARRRLTGAAAASLAAPVLLARSRNAIA
                     GAQQYEPLADAVRAALAAQIADRTPPTRTFARIEERLAFLDWLGAMGERLRQRKPDYQ
                     TRVEFLRTLDYEATRAGLDRQMVLGLIQVESNFRKYVISTAGARGYMQVMPFWTSVIG
                     DGDTRKLFDMRTNLRYGCVIMRHYLDLEKGDLFLALGRYNGSRGRAEYPNAVLAAWKG
                     RWQYERGGPRGSV"
     gene            complement(1066..2814)
                     /locus_tag="AC16HB_16470"
     CDS             complement(1066..2814)
                     /locus_tag="AC16HB_16470"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR5608436.1"
                     /translation="MRASAYFISTLREAPADAEVVSHQLMVRAGMIRKLAAGIYSYLP
                     LGLRSIRRIETIIREEMNRAGAIELLMPVVQPAELWMESGRWYKMGPELLRLKDRHER
                     DFVVQPTSEEVIADIVRESIRSYKQLPVNFYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDDAGAMKSYRAMYDAYCRIFTRMGLKFRAVAADTGNIGGKASHEFQVIADTGE
                     DAIAYCPTSDYAANIEMAEALPLIPHRAPPAEPMTKTATPGKEKCEDVAALLGLPLAR
                     TVKSIVLAVEEKNSKGEPVDARIWLLLIRGDHELNEVKASKVPGLKDGFRFATEGEIR
                     GAFGCRPGYLGPIDTRKPVTVVADRTVANMSDFVCGANEEDYHFTGVNWGRDLPEPVV
                     ADLRNVVAGDPSPDGKGVLAIQRGIEVGHVFAGLPYPEAMNLTFTDESGTPAPMKMGC
                     YGIGVTRLLGAAIEQNHDARGIIWPDAIAPFEVVVCPVNYRQSEQVRQVADHLYAELM
                     SAGVDVILDDRGERPGAMFADWELIGMPHRITVGERGLKNGEVEYVARHTLRPIAVPL
                     ADAYAFVRRELAGART"
     gene            2914..3492
                     /locus_tag="AC16HB_16475"
     CDS             2914..3492
                     /locus_tag="AC16HB_16475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR5608437.1"
                     /translation="MLDKDGYRPNVGIILLNHRNEVFWGKRVRQHSWQFPQGGIKHGE
                     SPEQAMFRELYEEIGLRPEHVKIVARTREWLRYHVPEQWVRRELRGNYRGQKQIWFLL
                     RMLARDCDVSLRRSEKPEFDAWRWQEYWIPLDAVIEFKRDVYQQALAELSRFVFRPGR
                     RGFAHGRRRDERAGAETQEVATAPSDPPPGPS"
     gene            complement(3500..4624)
                     /gene="proB"
                     /locus_tag="AC16HB_16480"
     CDS             complement(3500..4624)
                     /gene="proB"
                     /locus_tag="AC16HB_16480"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR5608438.1"
                     /translation="MNGPSVVASARRLVVKVGSSLVTNEGRGLDLAAISRWAKEIAQL
                     NRDGKQVVLVSSGAIAEGMKRLGWKRRPRQMHELQAAAAVGQMGLAQAYETSFGALGK
                     RTAQVLLTHADLVDRVRYLNARSTLLTLLDLDVVAVINENDTVVTDEIKFGDNDTLGA
                     LVANLVEADALVILTDQHGLFSADPRRNPSATLIATAQAGDPALEEIAGGSGSAIARG
                     GMITKVLAAKRAARSGAHTVIASGREPNALVRLAAGERIGTELMAATAVLTARKQWLA
                     DHLQLKGRVIIDAGAARAVRDEGKSLLPIGVVEVVGEFSRGDVIACVAPDGREIARGL
                     ANYGAAEARQIARKPSSEIESTLGYSDEPELIHRNNLVVT"
     gene            complement(4621..5697)
                     /gene="cgtA"
                     /locus_tag="AC16HB_16485"
     CDS             complement(4621..5697)
                     /gene="cgtA"
                     /locus_tag="AC16HB_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR5608439.1"
                     /translation="MKFIDEARIEVSGGRGGNGCASFRREKFIPKGGPDGGDGGRGGD
                     VWAVADQNINTLIDYRYTRKHAAGNGENGRGSDCFGAGGHDVELRMPVGTVITDDGSG
                     MVIADLARHGQRALVAKGGRGGLGNLHFKSSTNRAPRQFTRGESGEHRLLRLELRVLA
                     DVGLLGMPNAGKSTLIAAVSNARPKVAEYPFTTLHPHLGMVRLAPEAGFVIADVPGLI
                     EGAAEGAGLGHQFLRHLSRTRLLLHVIDFAPLDDARDPVRDARTLVAELRKYDVGLAR
                     KPRWLVLNKIDLVPRAERAARVAEFRRRLRTRAPTFCVSAASGEGCRDLMWAVHRLLS
                     KSRDTTASSVNDDDVLASAAERVA"
     gene            complement(5764..6024)
                     /gene="rpmA"
                     /locus_tag="AC16HB_16490"
     CDS             complement(5764..6024)
                     /gene="rpmA"
                     /locus_tag="AC16HB_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5608440.1"
                     /translation="MAHKKAGGSSRNGRDSQAKRLGVKVFGGEAINAGGIIVRQRGTK
                     FHPGDNVGIGRDHTLFATIDGTVKFSVSGAQNHQTVSVLPRG"
     gene            complement(6036..6344)
                     /gene="rplU"
                     /locus_tag="AC16HB_16495"
     CDS             complement(6036..6344)
                     /gene="rplU"
                     /locus_tag="AC16HB_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5608441.1"
                     /translation="MYAVIRSGGKQYRVASGQKIKVESIVADVGAEVTLDQVLAVGDG
                     ADVRVGTPLVAGAVVKATVVAQGRHPKVRIFKMRRRKHFQKRAGHRQNFTELRIDSIS
                     "
     gene            6616..>7235
                     /locus_tag="AC16HB_16500"
     CDS             6616..>7235
                     /locus_tag="AC16HB_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5608442.1"
                     /translation="MRAVDGLIRERLASDVALIGTIADYIIGAGGKRLRPAVHLLVAR
                     ALGYRGTAHVVLAAVIEFIHTATLLHDDVVDESDLRRGRRTSNSMFGNAASVLVGDFL
                     YSRSFQMMVDVGHMRVMRILADSTNRIAEGEVLQLMNIHDPSVDEARYLRVVERKTAT
                     LFEAASRIGAVLAGADAVTEEHAAQYGSSLGTAFQMIDDLLDYSGN"
BASE COUNT         1212 a         2457 c         2263 g         1303 t
ORIGIN      
        1 aaggcatcgg gcgcggcatg cctggcgcgc cgaagcgctg acccacgaca cgaggcaagg
       61 ccagtcaccg cccgccggct cattcgtcga tctgacgctg ctgcctgcgc tggccgagca
      121 cagtatcgaa tcgggcctca atcggcacgc gatccttcgg ttggccttgg tctcgtcctc
      181 tggttgctgg atcggcaccc gctcgagctc gagatgcaag agcaggttct cgccgatgcg
      241 aaaacccagg tcgccctgcg ccgtcgcgcg gactctcgtc gtctccagat tgagagcgca
      301 gtcgcccgtc tgcgtcaggt tcagcgacga gaagcgcgtc cagttcagga ctaggtacga
      361 atcgatcgaa ctgtcgccag catcgtccga tcgagccggt gccaagtcaa ggcacctcaa
      421 acgctgccgc gcggaccacc tcgttcgtac tgccaccggc ccttccacgc ggccagcacg
      481 gcgttcgggt actcagcgcg gccgcggctg ccgttgtagc gacccagcgc gaggaagagg
      541 tcgccttttt ccaggtcgag gtagtggcgc atgatcacgc atccgtaccg caggttggta
      601 cgcatgtcga aaagtttgcg cgtgtcgcca tcgccaatca cgctggtcca gaagggcatc
      661 acttgcatgt agccgcgcgc gccggcggtc gaaatcacgt atttgcggaa attgctctcc
      721 acctggatca gtccaaggac catctgccga tcaaggcctg cgcgcgtggc ctcgtagtcg
      781 agcgtacgca ggaattcgac ccgggtctga taatcgggct tgcgctgtcg cagccgctcg
      841 cccatcgcgc cgagccagtc gaggaaggca agccgttctt cgatgcgggc gaaggtgcgc
      901 gtgggaggcg tgcgatcggc gatctgcgcg gccagcgccg cacgcacggc gtcggccaga
      961 ggttcatatt gctgcgcccc cgcgatagcg ttgcgcgagc gcgccagcaa caccggcgcg
     1021 gcaagggacg cggccgcggc accagtcaga cgtcggcgcg ccggatcagg tgcgggcgcc
     1081 tgcaagctcg cgccgaacga atgcgtatgc atcggccagg ggaacggcga tgggccgcag
     1141 cgtgtgccgc gcgacgtact cgacctcgcc gttcttcagc ccgcgctcgc ccacggtgat
     1201 ccgatgcggc atgccaatca gttcccaatc ggcgaacatc gcgcccggcc gctcgccacg
     1261 atcatcgagg atgacgtcca cgcccgcgct catgagttct gcatacaggt gatctgccac
     1321 ttgtcgcacc tgttccgact gccgatagtt caccgggcag acgacgacct cgaatggcgc
     1381 gattgcatcg ggccagatga tgcccctggc gtcgtgattc tgctcgatcg ccgcgccaag
     1441 cagcctggtc acaccgatgc cgtagcagcc catcttcatc ggtgcgggcg taccactctc
     1501 atcagtgaac gtcagattca tcgcttccgg gtagggcagc ccggcgaaca cgtggccgac
     1561 ctcgataccg cgctgaatcg ccagcacccc cttgccgtcc ggcgacggat cgcccgcgac
     1621 gacgttgcgc agatcggcca cgacgggctc gggcaaatcg cggccccagt tgacgccggt
     1681 aaaatgatag tcctcttcgt tggcgccgca cacgaagtcg ctcatgttcg cgacggtgcg
     1741 gtcggccact acagtgaccg gcttgcgcgt gtcgatggga ccgagatagc cggggcggca
     1801 accgaaagcg ccgcgaatct cgccctcggt agcgaagcgg aagccgtcct tcaagcccgg
     1861 caccttgctg gccttgactt cgttcagctc gtgatcgccg cggatcagaa gcagccagat
     1921 cctcgcgtcg accggctctc ccttgctgtt cttctcttcg actgcgagca cgatcgactt
     1981 gaccgttcgc gcgagcggca gaccgagcag ggcggcgacg tcctcgcact tttccttgcc
     2041 cggtgtggcc gtctttgtca tcggctcggc cggaggcgcg cgatgcggaa tcagcggcaa
     2101 tgcctcggcc atctcgatgt tggcggcgta gtccgaggtg gggcagtagg cgatcgcgtc
     2161 ctcgcccgtg tcggcaatca cctgaaactc gtggctcgcc ttgccaccga tattgccggt
     2221 atcggccgcg accgcgcgga acttgagtcc catgcgggtg aagatgcggc agtaggcgtc
     2281 gtacatcgcg cgataactct tcatcgcgcc ggcatcgtcg cggtcgaacg agtacgcatc
     2341 cttcatcgtg aactcgcgcc cgcgcatcac gccgaagcgc ggccgtcgct cgtcccggaa
     2401 ctttgtctgg atgtgataga agttcaccgg caactgtttg tagctgcgga tcgactcgcg
     2461 cacgatatcg gcgatcacct cctccgatgt cggttggacc acgaagtcac gctcgtgccg
     2521 atccttcagc cgcagaagct cggggcccat cttgtaccag cgccccgact ccatccacag
     2581 ctcggccggc tgcaccaccg gcatcagcaa ttcaatggca ccggcccgat tcatttcctc
     2641 gcggatgatc gtttcgatgc gacggatcga gcgcagcccg agcgggagat agctgtatat
     2701 gccggccgcg agcttgcgga tcatgccggc gcgcaccatc agctggtggc tgacgacttc
     2761 ggcgtcggcc ggagcttccc ttagagtcga gatgaagtag gcggaggcgc gcatcgcgtc
     2821 gaaggtgcgt ccaggaccgt caaaaactct gtggccgcat cgtgaaacgc ggacctataa
     2881 tcggccgcag atgatatagc ttcaggtgtt ggcatgctgg acaaggacgg ctatcgcccg
     2941 aacgtcggca tcatcctgct caatcaccgg aacgaggtct tctggggcaa gcgcgtacgg
     3001 cagcactcct ggcagttccc acaaggcgga atcaagcacg gcgagtctcc tgaacaagcg
     3061 atgtttcgcg agctgtacga ggagatcggc ctcaggcccg agcacgtgaa gatcgtcgcc
     3121 cgcacgcgcg aatggcttcg gtaccacgtt cctgagcagt gggtgcggcg cgaactgcgc
     3181 ggcaactacc gcggacagaa gcagatttgg ttcctgctgc gcatgctggc gcgcgactgc
     3241 gacgtgtcgc tgcggcgatc ggaaaagccg gaattcgacg cttggcgctg gcaggagtac
     3301 tggatcccgc tcgatgcggt gatcgaattc aagcgcgatg tctatcagca ggcactggcg
     3361 gaattgtcgc gcttcgtttt ccgccccgga cgacggggct tcgcccacgg gcgccgccgc
     3421 gatgaacggg cgggcgcgga aacgcaggaa gtcgctactg ccccaagcga cccgccgccc
     3481 ggcccttcct gacccggcgt caagtcacga cgagattgtt cctgtgaatc agctcgggct
     3541 cgtcggagta gccgagggtt gactcgatct cgctgcttgg cttgcgagcg atctgccggg
     3601 cctcggcggc gccgtagttc gcgaggccgc gcgcgatctc gcggccgtcc ggcgctacgc
     3661 atgcgatcac gtcaccgcgc gagaattcgc ctacgacctc gaccacgccg atcggcagca
     3721 ggctcttgcc ctcgtcgcgc acagcgcgcg ccgcgccggc gtcgatgatc acgcgcccct
     3781 tcagctgcag gtgatcggcc agccactgct tgcgcgccgt caatacggcg gtcgccgcca
     3841 tcagctctgt gccgatgcgc tcgcccgctg ccaaccgcac gagcgcattc ggctcgcggc
     3901 cgctggcgat cacggtatgt gctccacttc gcgcagcccg cttggcggcc agcaccttgg
     3961 tgatcatgcc gccgcgcgcg atcgcgctgc cgctgccgcc ggcgatctct tccaatgccg
     4021 ggtcgccggc ctgcgccgtc gcgatcagcg tggcagaagg attgcgccgc ggatcggcgc
     4081 tgaacagccc atgctggtcg gtcaggatca ccagtgcatc ggcctcgacc agattggcca
     4141 ccagcgcgcc gagcgtgtcg ttgtcgccaa acttgatttc gtcggtgacc acggtgtcgt
     4201 tctcgttgat cactgcgacg acgtccagat cgagcaacgt cagcagcgtc gagcgcgcgt
     4261 tcaggtagcg cacgcgatcg acgaggtcgg cgtgcgtcag aagcacctga gcggtgcgct
     4321 tgccgagcgc gccgaagctt gtttcatacg cctgcgcgag gcccatctgt ccgaccgcgg
     4381 ccgccgcctg caactcgtgc atctgcctcg gtcgccgctt ccagccgagg cgcttcatcc
     4441 cttcggcgat cgcgccgctg gacaccagca ccacctgctt gccgtcgcgg ttcagctgcg
     4501 cgatctcctt cgcccagcgc gagatcgccg cgagatccag cccccgaccc tcgttcgtga
     4561 caaggctgct gccgaccttg acgaccagtc gccgcgcgct ggcaaccacg gaggggccgt
     4621 tcacgctacg cgctccgcgg ccgatgccaa cacgtcatcg tcattcacgg aagacgcggt
     4681 cgtgtccctc gatttcgaca gaagtcgatg cacggcccac atcaggtcgc gacacccttc
     4741 gccgctcgca gccgacacgc agaaggtcgg cgcccgcgtc cgcaatcggc ggcggaattc
     4801 cgccacgcgc gcagcgcgct cagcgcgggg cacgagatcg atcttgttca gcaccagcca
     4861 gcgcggcttt cgcgcaagcc ccacgtcgta tttcctcagt tcagcgacga gcgtgcgcgc
     4921 gtccctgacc gggtcacgcg catcgtcgag cggcgcgaaa tcgatcacgt gcagcagcaa
     4981 cctcgtgcgg ctcagatgcc gcaggaactg gtggccgagc ccggccccct cggccgcgcc
     5041 ttcgatcaga ccgggcacgt cggcgatcac gaagccggcc tcgggcgcca gtcgaaccat
     5101 tccgaggtgc ggatgcagcg tcgtaaacgg atactcggcg accttcggac gggcgttcga
     5161 gaccgcggcg atcagcgtcg acttgcctgc gttcggcatg cccagcaaac cgacgtcggc
     5221 gagcaccctg agttcgaggc gcagcaggcg atgctcgcca gattctccgc gcgtgaactg
     5281 acgcggtgcc cggttcgtgc tcgacttgaa gtgcagattg ccgagcccgc cgcgcccccc
     5341 cttggccacc agcgcgcgtt gcccgtgccg ggccagatcg gcgatcacca tcccactccc
     5401 gtcgtcggtg atcacggtac cgaccggcat ccgcagttcg acgtcgtgtc cgccagcacc
     5461 gaagcagtcg gaacctctgc cgttttcgcc attgccggcg gcatgtttac gcgtgtagcg
     5521 atagtcgatc agtgtgttga tgttttgatc cgcgactgcc catacgtcgc cgccacgccc
     5581 gccgtcgccg ccatcgggcc ctcccttcgg gatgaatttc tcgcgtcgaa acgaagcaca
     5641 cccgttgccg ccacggccgc ccgacacctc gattctcgct tcgtcgatga acttcacaat
     5701 gaaaaaagcc ctgccgttgt cggcagggct ttcggctgat cgcgtgctct gccccggtcg
     5761 tggtcaccca cgcgggagaa cgctgaccgt ctgatgattc tgcgcgccgc tgaccgagaa
     5821 cttgacggtc ccgtcgatcg tagcgaacag cgtgtgatcg cgcccgatac cgacgttgtc
     5881 gccaggatga aacttcgtgc ctcgctggcg aacgatgatg ccgccagcat tgatggcctc
     5941 cccgccgaac accttgactc cgagtcgctt ggcctgggag tcacgaccgt tgcgggacga
     6001 cccgcctgct ttcttgtgag ccatctcgtt cctcgtcagc tgatcgaatc gatccgcaat
     6061 tcggtgaagt tctgcctgtg gccggcgcgc ttctggaagt gcttgcggcg gcgcatcttg
     6121 aagatccgga ccttcggatg gcggccctgc gccacgacgg ttgccttgac aacggcacct
     6181 gccaccaagg gggtgccgac ccgcacatcc gcgccatcgc cgacggcgag aacctgatcg
     6241 agcgtcactt ccgcgccaac gtcggcaacg atcgattcaa ccttgatctt ctgtccactg
     6301 gcgacgcggt actgcttgcc gccgctcctg atcaccgcat acatgactct ggctcctgcc
     6361 gctcgccgta gcagcccggc gaaaatggca atcgattcgg gaaagctttg aattttctca
     6421 cgtccacgcc ggcctggtca aacgagcgga acgtggccgc ggccggccgc aatccccggc
     6481 cgacgccaac gagccggcac ctataatggc cggtgcaatc cgaggtcctt cgccttgtct
     6541 gaactgcaaa ctccgcccga atcgcagtcg tcgaccgatg gactggcgcg cctgctggcg
     6601 ccgattgccg acgacatgcg ggcggtcgac ggcctgatcc gggagcgact tgcttccgac
     6661 gtcgcgttga tcggcacgat cgccgattac atcatcggcg ccggcggcaa gcgcctgcgg
     6721 ccggcggtcc atctgctggt ggcgcgcgcg cttggctacc gcggaaccgc ccatgtcgtg
     6781 cttgccgcgg tcatcgaatt catccacacg gcgaccctgc tgcatgacga cgtcgtcgac
     6841 gagtcggact tgcggcgcgg gcgccggacg tcgaattcga tgttcggcaa cgccgcgagc
     6901 gtcctggtcg gagacttcct gtactcccgt tcgtttcaga tgatggtcga cgttggccac
     6961 atgcgcgtga tgcgcatcct ggccgattcg accaaccgca tcgccgaagg agaggtgctg
     7021 cagttgatga acatccacga tccatcggtg gacgaggcac gttacctcag ggtcgtcgag
     7081 cgcaagacgg ccacgctttt cgaggccgcg agccggatcg gcgctgtcct tgcgggggcg
     7141 gatgccgtga ccgaggagca tgcggcgcag tatggctcga gcctcgggac cgccttccag
     7201 atgatcgatg atctgctcga ttattccggc aacat
//
LOCUS       DBNHBY010000695         3686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1810165, whole genome shotgun sequence.
ACCESSION   DBNHBY010000695 DBNHBY010000000
VERSION     DBNHBY010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1810165"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /gene="tkt"
                     /locus_tag="AC16HB_16505"
     CDS             complement(<1..1477)
                     /gene="tkt"
                     /locus_tag="AC16HB_16505"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5608443.1"
                     /translation="MPDTSAATGSALQRQMCNAIRALAMDAVQKANSGHPGMPMGMAE
                     IALALWTRHLEHNPTNPHWPNRDRFVLSNGHGSMLQYALLHLAGYDLPLQELINFRQL
                     HSRTPGHPERGITPGVETTTGPLGQGFANAVGMALAEKLLAAEFNRPGFEIVDHRTYA
                     FVGDGCLMEGISHEAASLAGTWKLAKLIAIYDDNGISIDGEVKHWFTDDTAARFRAYR
                     WNVIGPVDGHDVDTVDRAIAQAKQSDRPTLIVAKTVIGKGSPHRAGSEKAHGEALGEA
                     EVAATRAALGWTYPPFEVPAEVYAAWDARAAGAARERAWQQSFDAYAQRYPTAAAELS
                     RRLRGDLPPNFADVLAGAIAAAAGKREAVATRKASQLALEVLAETVPEMLGGSADLTG
                     SNLTRWSKAKPLHVDGEITPGRHIHFGVREFGMNAIANGIALHGGFIPFVGTFLVFSD
                     YGRNALRMAALMKQRAIFVFTHDSIGLGEDGPTHQAVEHAAS"
     gene            1866..3197
                     /gene="speE"
                     /locus_tag="AC16HB_16510"
     CDS             1866..3197
                     /gene="speE"
                     /locus_tag="AC16HB_16510"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR5608444.1"
                     /translation="MQGLHLTGDLFDCGCSASLLTDLATLSKLCHDATVDAGLTIVDE
                     KYHVFPDWQGQPGGITGTILLAESHLAIHTWPERRGVTLDVYVCNFTDDNSGKAQQLF
                     DTLLVAFRPRQQVVNRINRGDLSASSVDAAAAARTDGPGMKGIAQAGQQKDELIFDWL
                     NAHSGYGYTAKAQLATIQSPYQRVEVYDTHQFGRLFRLDGRLMTSEGDEFFYHECMTH
                     PALLTHPNPQSVLVIGGGDGGSSEEIFKHPSVKRIVMAELDPVVIDISRKWLREIHKG
                     ALDDPRLEIRIGDGFEFVKNCTERFDLVVLDLTDPDTPAFQLYSEEFFRQCQRVLTPG
                     GLMTMHLGSPVYQAATVKKNAASLRKVFKQVHPMALYIPLYGSLWCLGIASDSANPRT
                     LSSETLAQRLRERRISGLKYYNAELHAGLFALPNFVKELTDGPVVAAKLAA"
     gene            3286..>3686
                     /locus_tag="AC16HB_16515"
     CDS             3286..>3686
                     /locus_tag="AC16HB_16515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR5608445.1"
                     /translation="MLRLRANNALTLFNGRGGEYAGRLDADGHARIERFDAVERESPL
                     ALTLIQALVAGDKLDWVIEKAVELGVARIIVVPTERSVIRLAEDRLLRRHTHWREIVV
                     AACCQCGRNRLPDIAFVRSWREALNSTDAMQR"
BASE COUNT          613 a         1231 c         1225 g          617 t
ORIGIN      
        1 gcgacgcggc gtgctcgacc gcctggtgcg tcgggccgtc ttcgcccagc ccgatcgaat
       61 cgtgcgtgaa gacgaagatc gcgcgctgct tcatcaacgc cgccatccgc agcgcgttgc
      121 gcccgtagtc agagaagacc aggaacgtgc cgacgaacgg gatgaaaccc ccgtgcagcg
      181 cgatgccgtt ggcgatcgca ttcatgccga actcgcgcac gccgaagtgg atgtgccggc
      241 ccggcgtgat ctcgccgtcg acgtgcagcg gtttcgcctt actccagcgg gtcaggttgc
      301 tgccggtgag gtcggcggag ccgccgagca tctccggcac tgtctcggcc agcacctcga
      361 gcgcgagctg cgacgccttg cgtgtggcca cggcctcgcg cttgcctgcg gctgccgcaa
      421 tcgcgcccgc cagcacgtcg gcgaagttcg gcggcagatc gccgcgcagc cgccgcgaaa
      481 gctcggcagc cgccgtaggg taccgctgcg cgtaggcatc gaacgactgc tgccaagcgc
      541 gctcgcgcgc tgcaccggcc gcgcgcgcgt cccacgcggc gtagacctcg gccggcacct
      601 cgaacggcgg gtacgtccag ccgagcgccg cgcgcgttgc cgcgacctcg gcctcgccca
      661 gcgcctcgcc atgcgccttc tcgctgccgg cacggtgcgg cgagcccttg ccgatcacgg
      721 tcttggcgac gatgagcgtc gggcggtcgg actgcttcgc ctgcgcgatc gcccgatcga
      781 ccgtgtcgac atcgtgcccg tcgaccggtc cgatcacgtt ccatcgatac gcgcggaagc
      841 gcgccgcggt gtcgtcggtg aaccagtgct tgacctcgcc gtcgatagag atgccgttgt
      901 cgtcgtagat cgcgatcagc ttggcgagct tccacgtgcc ggcgagcgac gcggcctcgt
      961 gcgagatgcc ctccatgagg cagccgtcgc cgacgaaggc gtaagtacgg tgatcgacga
     1021 tctcgaagcc gggccgattg aattccgccg ccagaagctt ctccgccaac gccatcccca
     1081 ccgcgttggc aaagccctgg ccgagcggac cggtcgtcgt ctccactccc ggcgtgatgc
     1141 cgcgctccgg atggcccggc gtgcggctgt gcagctggcg gaagttgatt aattcctgca
     1201 gcggcaggtc gtagccggcg aggtgcagca gcgcatactg cagcatcgaa ccgtgcccat
     1261 tggacaggac gaaacggtcg cgattgggcc agtgcgggtt ggtcgggttg tgctccaggt
     1321 gccgagtcca cagcgccagc gcgatctccg ccattcccat gggcatgccc ggatggccgg
     1381 agttggcctt ctgcacggcg tccatcgcga gcgcgcgaat ggcgttgcac atctggcgct
     1441 gaagcgcgct gccggtcgcg gctgaggtat ctggcatggg atcgggattg gattgcgctc
     1501 gccgagcggc cgctacgggc gttcgggacc gaaaagaaag tttatcaggc gcccctgctc
     1561 gaccgtgctt ggccgcgttc gcacgcgtag agcgacttta ggcgcgtcat tcgagaagaa
     1621 gttgccctgc atcactttcg tagagcggcg aattccatta gcattgcgga tcaaatttcg
     1681 gtccggaaat ccggaagcaa cgggcgccag ccgtatcggc aaatctcccc cgccagatcc
     1741 aatcgacgca gtggagcatc actgcactga tccagcgccg cgcaccagcg ttgggtcccg
     1801 gaccgatggg ttgcggttca tagccgtgat tcaggtcgat tgggcggacc tgctggggga
     1861 cgaagatgca aggacttcat ctaactggcg atctgttcga ctgcggatgc agcgccagcc
     1921 ttctcactga tctcgccacg ctctcgaagc tgtgccacga cgcgaccgtc gacgcggggc
     1981 tcacgatcgt cgacgagaag taccacgtgt ttccggactg gcagggccag ccgggcggta
     2041 tcaccggcac gattctgctc gccgaatcgc acctggcaat ccacacctgg cccgagcggc
     2101 gtggcgtcac gctcgacgtc tacgtgtgca acttcaccga cgacaactcg ggcaaggcgc
     2161 agcagctttt cgacacgctg ctggtcgcgt tccggccgcg gcagcaggtc gtcaaccgca
     2221 tcaaccgtgg cgacctgtcg gccagttccg tggatgccgc ggccgctgcg cgcaccgacg
     2281 gcccgggcat gaaggggatc gcgcaggccg gtcagcagaa ggacgagctg atctttgact
     2341 ggctcaatgc gcactccggc tacggctaca cggcgaaggc gcaactggcg acgatccagt
     2401 cgccgtacca gcgcgtggag gtgtacgaca cgcaccagtt cggcagactg ttccggcttg
     2461 acggtcggct gatgacgtcg gagggcgacg agttcttcta tcacgagtgc atgacgcacc
     2521 cggcgctgct gacgcatccc aacccgcaga gcgtgctggt gatcggcggc ggcgacggcg
     2581 ggtcctcgga ggagattttc aagcacccga gcgtcaagcg catcgtgatg gccgagctcg
     2641 acccggtggt gatcgacatc agtcgcaagt ggctgcgcga gatccacaag ggagcgcttg
     2701 acgatccgcg gctggaaatc aggattggcg acgggttcga attcgtgaag aactgcaccg
     2761 agagattcga cctcgtcgtg ctcgacctga ccgaccccga cactccggcg tttcaactgt
     2821 actcggagga attcttccgc cagtgccagc gcgtgctgac gcccggcggc ctcatgacga
     2881 tgcacctggg ctcgccggtg taccaggcgg caacggtgaa gaagaacgcc gccagtttgc
     2941 ggaaggtgtt caagcaggtg cacccgatgg cgctgtacat cccgctgtac ggatcgctgt
     3001 ggtgcctcgg catcgccagc gacagtgcca atccacgcac gctgtcgagc gagaccctcg
     3061 cgcagcgttt gcgcgagcgg cgcatcagtg gcctgaagta ctacaacgcc gaactgcatg
     3121 cgggcctgtt cgcgctcccg aacttcgtca aggaactgac cgacggcccc gtcgtcgcgg
     3181 ccaagctcgc cgcttagcgc cgcgccgcgc cttttcgttg acggcgacct cgccgtcggc
     3241 gcgcgcgtcg aactgcccgc ccccgtcgcg caccacgcgc tgcgcgtgct gcggctgcgc
     3301 gcgaacaacg cgctgacgct cttcaacggc cgcggcgggg aatacgcggg acggctggac
     3361 gccgacggtc acgcgaggat cgagcgtttc gacgcggtcg agcgcgaatc tccgctcgcg
     3421 ctcacgctga tccaggcgct ggtcgcaggc gacaagctcg actgggtgat cgagaaggcc
     3481 gtcgaactcg gcgtcgcgcg catcatcgtc gtaccgaccg agcgcagcgt gattcggctg
     3541 gccgaagatc ggctcttgcg ccgccataca cactggcgcg agatcgtcgt ggccgcctgc
     3601 tgccagtgcg gacgcaaccg gctgccggac atcgcattcg tacgctcgtg gcgcgaggcc
     3661 ttgaactcga ccgacgcgat gcagcg
//
LOCUS       DBNHBY010000696         3801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_593155, whole genome shotgun sequence.
ACCESSION   DBNHBY010000696 DBNHBY010000000
VERSION     DBNHBY010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_593155"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1126)
                     /gene="nudC"
                     /locus_tag="AC16HB_16520"
     CDS             complement(305..1126)
                     /gene="nudC"
                     /locus_tag="AC16HB_16520"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR5608446.1"
                     /translation="MLTTPENFTPLITAAATAQSHAFVFRGNELLIRAADLALPDPRL
                     AATLVEAGARMHPVGLLGDRYCCASWVEPHVAPPPGYAFSGLRRLFGTLDEPLLAVAA
                     RAFQIAEWARTHRYCGACGAPTQLAAGERCFRCAACGHVSYPRISPAMMVLVRRGAAI
                     LLARHLASPTGRFTALAGFVEPGESIEDAVHRETFEEVGLRVRDLRYFASQSWPFPHS
                     LMVAFTAEYAGGELAIDSNEIAEARWFGLDDEFPEIPPAVSIAHALINAHRPRRR"
     gene            complement(1126..2448)
                     /locus_tag="AC16HB_16525"
     CDS             complement(1126..2448)
                     /locus_tag="AC16HB_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608447.1"
                     /translation="MIGDALSIFAAARDAADAIGLHLGARRFTFAELAELVHARMREL
                     DARPRAGALFPLTAANALDTVVTLYALLEQRVPALLLHPRLTDSERAAQLVSAARAQN
                     VPADAAAVIYTSGTSGTPRGAMLTRSALVASANASAANLGWRDDDCWLLAMSLARVGG
                     LSIVTRCLIARRPFALVAQFDADELPSLIDAQRATLVSLVPTMLSLVLDAHPHWRAPP
                     FLRAILLGGAAAPGRLLQRAQRRALPIMITYGATETCSQVVATPYEHRFHAAEHGAGR
                     PLPGAEIRIVDGRIHARGSMLMAGYLGEPALERDAWFDTGDLGEIDARGCLRVHAREG
                     DLIVSGGENVYPAEVERVLEACPGVVAAAVFGTPDETWGQKVAAAVVVDSQTRVEAAL
                     HEFVCARLAPAKRPRAIHIVAELPHTPAGKLDRAALARMFETSARAEG"
     gene            complement(2445..3287)
                     /gene="menB"
                     /locus_tag="AC16HB_16530"
     CDS             complement(2445..3287)
                     /gene="menB"
                     /locus_tag="AC16HB_16530"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR5608448.1"
                     /translation="MDAKTAAPLPTWTAVTGYRDIIYAKAEEGIAKVTINRPEVRNAF
                     RPQTVQELQAAFTDARDDPNVGVIILTGQGKEAFCSGGDQRVRGEGGYVGDDGVPRLN
                     VLDLQRQIRTLPKPVVAMVAGYAIGGGHVLHMVCDLTIAADNARFGQTGPKVGSFDGG
                     YGASYMARIVGQKKAREIWFLCRQYDAQQALDMGLVNCVVPYERLEAETVQWCREMLA
                     NSPIALRCLKAALNADCDGQAGLQELAGNATLLFYLSEEAKEGRDAYLQKRKPDFRKF
                     PRFP"
     gene            complement(3299..>3801)
                     /locus_tag="AC16HB_16535"
     CDS             complement(3299..>3801)
                     /locus_tag="AC16HB_16535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR5608449.1"
                     /translation="AGNGTTSHLAGELFKMMTGVDIVHVPYRGGALAVVDLIGGQVQM
                     MIDVMPNAYPLAKDGKVRGIAVTTAERFPGAPEYPTFAESGLPGFEVSAWDGVFAPAG
                     TPEPIVTKLNAAIRQALDDRDLRLALIARGAQPVPASPEEFAQRVAASAQKWSRLVRA
                     AGAKVD"
BASE COUNT          567 a         1375 c         1302 g          557 t
ORIGIN      
        1 tcggtcacgt cgaactgacg gcgacggact tcgtgatgta gcggtaccgg ggtccggcgc
       61 gtttcgagcg tcgggcgtca gcgcggctgc ggatcgcttc gacctccgcc ggcggccgat
      121 gcgaatggcg ccgccggcga gcgtcgacga cctggctcgc gatgtcgtgc gcgatggact
      181 tcgacaggac cccgtcgtcg agcttacgca cgacaacaac aacttcactc ccgcgtgggg
      241 aggcagatag tcgctgttcc tctttccggt cgtgcctcag cgctggccga tcccgggacg
      301 cgtcctagcg ccgccgcggc cgatgcgcgt tgatcaatgc gtgcgcgatc gacacggcgg
      361 gcgggatctc cgggaactcg tcgtcgaggc cgaaccagcg tgcctcggcg atctcgttgc
      421 tgtcgatcgc cagttcgccg cccgcgtact cggcggtgaa cgcgaccatc agcgaatgcg
      481 gaaacggcca cgactggctg gcgaagtacc tgaggtcccg cacgcgcagg ccgacctctt
      541 cgaaggtctc gcgatgaacc gcgtcctcga tcgattcgcc cggctcgacg aacccggcga
      601 gcgcggtgaa gcggccggtc ggcgacgcca gatgccgcgc gagcaggatg gccgcgccgc
      661 gccggacgag caccatcatc gccggcgaaa tgcgcgggta cgacacgtgc ccgcacgccg
      721 cgcacctgaa gcagcgctcg cccgcggcga gctgcgtcgg tgcgccgcag gcgccgcaat
      781 agcgatgcgt gcgcgcccac tcggcaatct ggaacgcgcg ggctgcgacc gccagcagcg
      841 gttcgtcgag cgtgccgaac agccggcgca ggccgctgaa cgcgtagcct ggcggcggcg
      901 cgacgtgcgg ctcgacccac gaggcgcagc agtagcgatc gccgagcagg ccgaccggat
      961 gcatccgcgc gcccgcctca accaacgtcg cggcgagccg cgggtcgggc agcgcgaggt
     1021 cggcggcgcg gatcagcagt tcgttgccgc gaaagacgaa tgcgtgcgac tgtgcggtcg
     1081 cggcggcagt gatgagcggc gtgaagttct ccggggtcgt cagcatcaac cctccgcgcg
     1141 cgccgatgtt tcgaacatgc gcgcgagtgc cgcgcggtcg agcttgcccg ccggcgtgtg
     1201 cggcagctcg gcgacgatgt ggattgcacg cggccgcttg gccggcgcca gtcgcgcgca
     1261 gacgaattcg tgcaaggcgg cttcgacgcg cgtctgcgaa tcgacgacca ccgccgccgc
     1321 gaccttctgg ccccaggtct cgtcgggtgt gccgaacacc gctgccgcga ccacgcccgg
     1381 acacgcctcc agcacgcgtt cgacttcggc cggatacacg ttctcgcccc cggagacgat
     1441 caggtcgcct tcgcgcgcgt gcacgcgcag gcagccgcgc gcatcgatct cgccgaggtc
     1501 gccggtatcg aaccacgcat cgcgctcgag cgcgggctcg ccgaggtagc ccgccatcag
     1561 catcgacccg cgcgcatgga tgcggccatc gacgatccgg atctccgcgc cgggaagcgg
     1621 cctgccggcg ccgtgctcag ccgcgtggaa gcggtgttcg tatggggtgg cgacgacctg
     1681 cgagcaggtt tccgtcgcgc cgtacgtgat catgatcggc agcgcgcgcc gttgggcgcg
     1741 ctgcaacagg cggcccggcg ccgccgcgcc cccgagcagg atcgcgcgca gaaacggcgg
     1801 cgcgcgccag tgcgggtgcg catcgagcac gaggctgagc atcgtcggca ccagcgacac
     1861 cagcgtggcg cgctgcgcat cgatcagcga cggcaattcg tcggcatcga actgcgccac
     1921 cagcgcgaac ggccgtcgcg cgatcaggca gcgtgtgacg atcgaaagcc cgcccacgcg
     1981 ggcgagcgac atcgcgagca gccagcagtc gtcgtcgcgc cagccgaggt tggccgcact
     2041 cgcattggcc gatgcaacca gcgccgagcg cgtcagcatc gcgccgcgcg gcgtgccgct
     2101 cgtgccggag gtgtagatca ccgccgccgc gtccgccggc acgttttgcg cgcgcgccgc
     2161 cgataccagt tgcgccgcgc gctcggaatc ggtgagccgc ggatgcagca acagagccgg
     2221 aacgcgctgc tccagcagcg catacagcgt gacgacggtg tcgagcgcat tggcggccgt
     2281 cagcgggaac agcgcgccag cgcgcggtcg cgcatcgagt tcacgcatgc gggcgtgcac
     2341 gagctccgcc agttcggcga acgtgaagcg gcgcgcgccg agatgcaggc cgatcgcgtc
     2401 cgccgcgtcg cgcgcggcgg cgaagatcga cagtgcgtcg ccgatcaagg gaagcgcggg
     2461 aacttgcgga agtccggctt gcgcttctgc aggtaggcgt cgcgcccttc cttcgcctct
     2521 tcggacaggt agaacagcag cgtcgcgttg cccgcgagtt cctgcagccc ggcctgccca
     2581 tcgcagtcgg cgttcagcgc cgccttcaga cagcgcagcg cgatcgggct gttggccagc
     2641 atctcgcggc accactgcac cgtttccgcc tcgagccgct cgtacggcac cacgcagttg
     2701 accaagccca tgtcgagcgc ctgctgcgcg tcgtactgcc ggcacaggaa ccagatctcg
     2761 cgcgccttct tctgtccgac gatgcgcgcc atgtaagacg cgccgtagcc gccgtcgaag
     2821 ctgccgacct tcgggcccgt ctgcccgaag cgcgcgttat cggcagcgat cgtcaggtcg
     2881 cacaccatgt gcagcacgtg gcccccgccg atcgcatacc cggccaccat cgcgacgacc
     2941 ggcttgggca aggtgcggat ctgtcgctgc aggtcgagca cgttcaggcg cggcacgccg
     3001 tcgtcgccga cgtagccgcc ctcgccgcgt acgcgctggt cgccgccgga gcagaacgcc
     3061 tccttgccct gccccgtgag aatgatcacg ccgacgttcg gatcatcccg cgcgtcggtg
     3121 aacgccgcct gcagctcctg caccgtctgc ggccggaacg cgttgcgcac ctcggggcga
     3181 ttgatcgtga ccttggcgat accctcctcg gccttcgcat agatgatgtc cctgtaaccg
     3241 gttaccgcgg tccaggtcgg cagcggtgcc gccgtcttcg cgtccatcct gctctccttc
     3301 aatcgacttt cgcgcccgca gcgcgcacga gccggctcca cttctgcgcg ctcgctgcga
     3361 cacgttgcgc gaattcctcc ggcgacgccg gcaccggctg cgcgccgcgc gcgatcagcg
     3421 ccaggcgcag gtcgcgatcg tcgagcgcct gccggatcgc cgcgttcagc ttcgtaacga
     3481 tcggctccgg cgtgccggcg ggcgcgaaga caccgtccca cgcgctgacc tcgaagcccg
     3541 ggagcccgga ttcggcgaag gtcgggtact ccggcgcgcc gggaaagcgc tcggccgtcg
     3601 tcaccgcgat gccgcgcacc ttgccgtcct tcgcgagcgg ataggcgttc ggcatcacgt
     3661 cgatcatcat ctgtacctgc ccgccgatca ggtccaccac cgccagggcg ccgccgcggt
     3721 acggcacgtg cacgatgtcg actcccgtca tcatcttgaa cagctcgccg gccagatgcg
     3781 acgtcgtgcc gttgccggcc g
//
LOCUS       DBNHBY010000697         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_525570, whole genome shotgun sequence.
ACCESSION   DBNHBY010000697 DBNHBY010000000
VERSION     DBNHBY010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_525570"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16HB_16540"
     CDS             complement(<1..311)
                     /locus_tag="AC16HB_16540"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5608450.1"
                     /translation="MTHDDPADQASGSATTVARGRTAEDLALRHLQSAGLVLLVRNYR
                     CRTGELDLVMLEPETRVLVLVEVRSRSRRDFGSAAATVGHAKQRRCSLAARHLLLTRR
                     EL"
     gene            complement(363..2315)
                     /locus_tag="AC16HB_16545"
     CDS             complement(363..2315)
                     /locus_tag="AC16HB_16545"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR5608451.1"
                     /translation="MLHVSLRVGQARCGLRLWLAVLGLMLASVAHAQSALPSDSQADA
                     LAAAGRPLEAAARYEQLAKRGLFTWDAGYALRAAGAYLDAGEPDEADRMLDKARSRAR
                     TIEERTWLGALDARLALDRGDAARALGALDTVPRPVPRYLAPRLLELRLRAQAAAGLQ
                     LAAVRTWVERAALLATPPERAANDRLLLDQLLLNPPSPAAAAAGTNALERGWLELPAL
                     LALAGRDKGSLSADAVQRLQQWRAQYPDHPGAAFLPSASASTAAAPAQPPTLAAGASA
                     RIALLLPLSGKQAAAGQAVRDGFAAAWFATGSVENRPRLDVYDTANGAAAAYQQAIAN
                     GAQAVVGPLLKEDVAALVAALPAGLPVATLALNSYPVATGAPPAFLYQFALDPEQEAR
                     AVAQRIAADGYVRGIALFPDSAWGQRVRDAFVAELQATGVVLTASQFYAPGASDFAGP
                     LRAALGRYGGAGDRPADRSQPAPSRNAAAEQAEGPQFAFIAATPQAARAIRPQLRFQM
                     TYDLPLYTTSDAWDASVRSAADMDGMVFPEMPWLLYGGQGAPELWEALQSGEWAGRGR
                     GRLRLFAFGYDAYRLVQQIGASGGAAGLDGLTGLLELDRSNGHVQRRLQFARIEGGRP
                     QPARTGAPVPMLAEPATNSGGQPRAP"
     gene            2341..>3146
                     /gene="rsmI"
                     /locus_tag="AC16HB_16550"
     CDS             2341..>3146
                     /gene="rsmI"
                     /locus_tag="AC16HB_16550"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR5608452.1"
                     /translation="MRDDSDDSTGGARCGALFVVATPIGNLQDLSPRGLACLRDADWL
                     LAEDTRHTRKLLEANGIERAPGTLESLHEHNERERTPELVARMVAGARIALVADAGTP
                     LLSDPGAELVAAAAAAGIDIVAVPGPCAAIAALSIAGLPTEAFVFEGFLRARPAARRA
                     ELLRLAHEPRTLVLYEAPHRLRETLADLAQAFGAERRASVARELTKRFEHVYRGTLAA
                     LAERSTHDADMTRGELVIVVHGASRSAAEEDTQHAQALRTLRALLDEQLPV"
BASE COUNT          456 a         1109 c         1152 g          429 t
ORIGIN      
        1 agttcccgcc gtgtcagcag caggtgccgt gcggccaacg aacaacgccg ctgcttggcg
       61 tgcccgacgg tcgccgcggc gctgccgaag tcgcggcgcg agcgggagcg cacttcgacg
      121 agcaccagca cgcgggtctc cggctcgagc atgacgaggt cgagttcacc tgtacggcag
      181 cggtagttgc gtacgagcag gaccaggcct gcggactgca ggtgacgcag cgcgaggtcc
      241 tccgcagtgc ggccccgcgc tacggtcgtg gctgaacccg atgcctgatc agcggggtcg
      301 tcgtgggtca tgggcctggc gctcggtgcc gcacgcaggg cggtcgggag cgccaagcct
      361 gctcaaggtg cgcgaggttg tccgcccgaa ttcgtggcgg gttccgcaag catcgggaca
      421 ggcgcgcccg tgcgcgccgg ttgcggccga cccccttcga tgcgcgcgaa ctgcagccgg
      481 cgctggacgt gaccgttgct gcggtcgagt tccagcagtc ccgtcaaacc gtcgaggccc
      541 gcggccccgc cggacgcgcc tatctgctgc accaggcggt aggcgtcgta gccgaacgcg
      601 aagagccgca accgaccacg tccacgtcct gcccactcgc cgctttgcaa ggcttcccac
      661 aactcgggtg cgccctggcc gccgtagagc aaccacggca tttcggggaa gaccatgccg
      721 tccatgtccg ccgctgaacg cacgctcgca tcccaggcat cggaggtggt gtacagcggc
      781 aggtcgtacg tcatctggaa gcgcaactgc gggcggatcg cgcgcgcggc ctgcggggtg
      841 gccgcgatga aggcgaactg cgggccctcg gcctgttccg ccgcggcgtt gcgcgacggt
      901 gcgggctggc tgcgatcggc aggccggtct cctgcgccac cgtagcgacc gagcgccgcc
      961 cgcagcgggc ctgcgaaatc gctggccccg ggtgcgtaga actgcgatgc agtcagcacc
     1021 acgccggtcg cctgcaattc ggccacgaat gcatcgcgca cccgctgccc ccaggcagag
     1081 tccgggaaca gcgcgatgcc gcggacgtag ccgtctgcag cgatgcgctg cgcaacggcg
     1141 cgcgcttcct gctcgggatc gagggcgaac tggtacagga acgccggcgg agcgccggtg
     1201 gcgacggggt acgagttcaa tgcgagcgtt gcaaccggca gacctgccgg cagtgcggcg
     1261 accagtgcgg cgacgtcttc cttgagcagc ggcccgacga cagcctgcgc tccgttcgca
     1321 attgcctgct gataggccgc agcggcgccg ttcgcagtgt cgtacacatc gaggcgcgga
     1381 cggttctcga cggagcctgt tgcaaaccac gccgctgcaa agccgtcgcg caccgcctgg
     1441 ccggcggcgg cctgtttgcc cgagagcggc agcagcagcg cgatgcgggc cgatgcaccg
     1501 gccgcgagcg ttggcggctg cgcaggcgca gcagccgtcg atgcgctcgc ggaaggcagg
     1561 aacgcagcgc ccgggtggtc cgggtactgc gcccgccact gctgcaggcg ctgcaccgcg
     1621 tccgcgctga ggctgccctt gtcgcgccct gcgagcgcga gcagggcagg caactcgagc
     1681 cagccgcgct cgagcgcatt ggtgcctgct gcggcagccg cagggctcgg cgggttcagc
     1741 agcaactggt cgagcagcag tcgatcgttc gcggcgcgct ccggcggcgt ggcgagcaat
     1801 gcggcgcgct ccacccacgt acgcacggca gcaagctgca gtcccgccgc agcctgggcg
     1861 cgcagccgca gctccagcaa tcggggtgcc agatagcgcg ggactggccg tggcacggtg
     1921 tccagcgcgc ccagcgcgcg cgctgcgtcg ccgcggtcca acgcgaggcg ggcgtcgagc
     1981 gcgccgagcc acgtgcgctc ctcaatcgtc cgcgcgcggc tgcgtgcctt gtcgagcatg
     2041 cgatccgctt cgtccggttc gccggcgtcg aggtaggcac cggctgcgcg cagcgcgtac
     2101 ccggcgtccc aggtgaacag gccgcgcttc gccagctgct cgtagcgcgc cgccgcttcg
     2161 agcggcctgc ctgcggctgc gagtgcatcc gcctgcgagt cggacggcag cgcggactga
     2221 gcatgcgcga cgctggcgag catcagcccc agcacggcaa gccacagccg caggccgcag
     2281 cgagcttgcc cgacgcgaag tgatacgtgc agcatgcggg tggacttccg ggaggcgcgc
     2341 gtgagggacg atagcgacga ttctacgggc ggtgcccggt gcggggcact cttcgtcgtc
     2401 gcgacaccga tcggcaacct gcaggatctc agcccgcgcg ggctggcctg cctgcgcgac
     2461 gccgactggc tgctggccga ggacacgcgt cacacgcgca agttgctcga ggccaacggc
     2521 atcgaacgcg cgccgggtac gctcgaatcg ctgcacgaac acaatgagcg cgagcgcacg
     2581 ccggaactgg tggcgcggat ggtggccggc gcccgcatcg cgctcgtcgc cgatgcaggc
     2641 acgccgctgc tgagcgaccc gggcgcggag ctcgtcgcgg ctgcggctgc ggctggcatc
     2701 gacatcgtgg ccgtgcccgg gccgtgtgcc gcgatcgcag cgttgtcgat cgcgggcctg
     2761 ccgacggaag cgttcgtgtt cgaggggttc ctgcgcgccc ggcccgccgc tcgccgggcc
     2821 gagctcctgc gactcgcgca cgagccacgc acgctcgtgt tgtacgaggc gccgcaccgg
     2881 ctgcgcgaaa cgctcgcgga cctcgcgcag gccttcggcg cggagcgtcg cgcgagtgtc
     2941 gcgcgcgaac tcaccaagcg tttcgagcac gtgtaccgcg gcacgctcgc tgcgctggcg
     3001 gagcgcagca cgcacgacgc agacatgacg cgtggcgagc tggtcatcgt cgtgcacggt
     3061 gcgtcgcgca gcgcggcaga ggaggacacg cagcacgcgc aggcgctgcg cacgctgcgc
     3121 gctctgctcg atgaacaact gcccgt
//
LOCUS       DBNHBY010000698         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1271088, whole genome shotgun sequence.
ACCESSION   DBNHBY010000698 DBNHBY010000000
VERSION     DBNHBY010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1271088"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="AC16HB_16555"
     CDS             <1..1547
                     /locus_tag="AC16HB_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5608453.1"
                     /translation="GWSGSDGSLPVAHNAIFAVGDDDQSIYRFRGANVGNMADFEREF
                     RVHNVVKLEQNYRSHGHILDAANALIRNNAQRLGKNLWTDAGEGEKVRVFEAGSDGLE
                     AAWLVDEVRGLIGEGHLRSEIAVLYRSNAQSRVIEHALFNAGIPYRVYGGLRFFERAE
                     IKHALAYLRLIENPADDTAFLRVVNFPPRGIGARTLEQLQDAARASNQSLYAAVGSMT
                     GRAGASLSAFVKLIDGVRFETANLPLKEIVAAVIDRSGLVAHFSNEKEGKERVENLEE
                     LVNAAVGFAAEEGYAQDAPATQSEAEAALPSPLAAFLSHASLEAGENQAAEGVDALQM
                     MTVHAAKGLEFHAVFITGLEEGLFPHENSVTEIGGLEEERRLMYVAITRARRRLYLSF
                     AQQRMLHGQTRYGIKSRFIDELPDGVLKWLTPRLREGAGLRYGAWHDEGVRAAVASAS
                     FGVTTARVETRNGYRIGQTVRHDRFGEGVIIKLEGSGNDARAKINFGAQGIKELLLSI
                     AKLQTA"
BASE COUNT          286 a          529 c          581 g          236 t
ORIGIN      
        1 ccggctggag tggaagcgac gggtccctgc ccgtcgcaca caacgcgatc ttcgcggtcg
       61 gcgacgacga ccagtcgatc tatcgctttc gcggcgccaa cgtcggcaac atggccgact
      121 tcgaacgcga gttccgcgtg cacaacgtcg tgaagctgga gcagaactac cgctcgcacg
      181 gccacatcct cgacgccgcc aacgcgctga tccgtaacaa cgcgcagcgg ctcggcaaga
      241 acctgtggac cgacgcgggc gagggcgaga aggtgcgcgt gttcgaggcc ggttccgacg
      301 gcctcgaagc cgcgtggctg gtcgacgagg tgagaggtct gatcggggag gggcacctgc
      361 gcagcgagat cgcggtgctg taccgctcca acgcgcagtc gcgcgtgatc gagcacgcgc
      421 tgttcaacgc cggcattccg tatcgcgtct acggcgggct gcgcttcttc gagcgcgccg
      481 agatcaagca tgcgctcgcg tacctgcggc tgatcgagaa cccggccgac gacaccgcgt
      541 tcctgcgcgt ggtgaatttc ccaccgcgcg gcatcggcgc gcgcacgctg gagcagttgc
      601 aggatgcggc gcgcgcgtcg aaccagagcc tgtatgcggc ggtgggctcg atgaccggcc
      661 gcgcgggcgc aagcctgtcc gcgttcgtca agctgatcga tggcgtgcgt ttcgaaaccg
      721 cgaatctgcc gctgaaggaa atcgtggcgg cggtgatcga ccgcagcggg ctcgtcgcgc
      781 acttcagcaa cgagaaggaa gggaaggagc gggtcgagaa cctggaggaa ctcgtcaacg
      841 ccgcggtcgg cttcgcggcg gaggagggct acgcgcagga tgcgccggcc acgcaatccg
      901 aagccgaggc tgcgctgccg tcgccgctgg ccgcgttcct gtcgcacgcc tcgctggagg
      961 cgggcgagaa ccaggccgcg gaaggcgtgg acgcgctgca aatgatgacg gtgcacgccg
     1021 cgaaggggct ggaattccac gcggtgttca tcaccgggct ggaggagggc ctgttcccgc
     1081 acgaaaacag cgtgaccgag atcggcggtc tcgaagagga gcggcggctg atgtacgtcg
     1141 cgatcacacg cgcgcgccgg cggctttatc tttcattcgc ccagcagcgg atgctgcacg
     1201 ggcagacgcg ctatggcatc aagtcgcgct tcatcgacga gctgcccgac ggcgtgctca
     1261 agtggctgac gccgcgcctg cgcgaaggcg cggggctgcg ctacggcgct tggcacgacg
     1321 agggcgtgcg cgcggcggtc gcgagcgcat cctttggcgt cacgaccgcg cgcgtggaaa
     1381 cgcgcaacgg ctaccgcatc ggccagaccg tgcgccacga ccgcttcggc gaaggcgtca
     1441 tcatcaagct cgaaggctcg ggcaacgacg cgcgcgcgaa gatcaacttc ggcgcgcagg
     1501 ggatcaagga actgctgctg tcgatcgcga agctgcagac ggcataggcc gtccgcagct
     1561 tcgcgtgccg cgaggactac gacactctcc ccaacccctc tcgccaggcg agaggggccg
     1621 aggctcgcct ca
//
LOCUS       DBNHBY010000699         9872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1201723, whole genome shotgun sequence.
ACCESSION   DBNHBY010000699 DBNHBY010000000
VERSION     DBNHBY010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1201723"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16HB_16560"
     CDS             complement(<1..194)
                     /locus_tag="AC16HB_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5608454.1"
                     /translation="MRFCSLGSGSEGNALVVEVHDGLTVTRLLVDNGFGPRALAKRLG
                     RASMAIDDLDAVVVTHEHSD"
     gene            complement(210..1352)
                     /gene="bamC"
                     /locus_tag="AC16HB_16565"
     CDS             complement(210..1352)
                     /gene="bamC"
                     /locus_tag="AC16HB_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR5608455.1"
                     /translation="MLNVTQAVRCAGLFVLAAALAGCSSLGLSTDKIDYKSVAPTKSL
                     EVPPDLSQIPRDDRYALSDRPGVASASAIGAARAPAAAVQTVAPATARARIERDGAER
                     WLAVDMTPEQAFVVVKEFWPSVGLVIEREDAAAGIVETTWAENRAKLPQDIIRRTIGR
                     LIDGLYSTGEQDKFRTRIERTPKNTAEVYISHRGMVEVYSSTQRDQTRWQPREPDHEL
                     EAEMLQRLLLRFLPATTTATAAPATAATAPAAAAAAHEIARIVRGADGKAQRVQIDEP
                     FDRAWRRVGLALDRGGFTVEDRDRTKGTYFVRYLDPEFEAKQRENQGFFSRMFGRDPK
                     IDPQQFRVQVAAAGDATSVTVLDKEGKPDGGATAQKILGQINEQLK"
     gene            complement(1375..2250)
                     /gene="dapA"
                     /locus_tag="AC16HB_16570"
     CDS             complement(1375..2250)
                     /gene="dapA"
                     /locus_tag="AC16HB_16570"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5608456.1"
                     /translation="MITGSLVAIVTPMREDGALDYDAYRRLIEWQIAEGTNGIVAVGT
                     TGESPTVDHDEHCELIRIAVETVRRRVPVIAGTGGNSTSEAIDLTQYAKKVGADASLQ
                     VVPYYNKPTQEGLYQHFRKVAETVDLPVILYNVPGRTVADLANDTTVRLAQIPGIVAL
                     KDATGDLARAADLLRRLPRSFAVYSGNDDSALALLLLGGHGVISVTANVAPRPMSDMC
                     RAALAGELATARALNNRLLPLHAKLFVESNPGPVKWALSKLGRCNAHVRLPLVPLSAT
                     NQPMVAAALQEAGIA"
     gene            complement(2311..2925)
                     /locus_tag="AC16HB_16575"
     CDS             complement(2311..2925)
                     /locus_tag="AC16HB_16575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5608457.1"
                     /translation="MIDPRARATAAVRNALVGAAHEGAEPSAWVERFAPLVPPGSRVL
                     DLACGHGRHALLFAAQRCLVDAVDIDPACADAFREAPQVAFRRADLENGPWPYRGRQF
                     DAIVVTNYLYRALFPVLADSLAPGGVLLYETFAVGNERYGKPSNPAFLLKPRELLDVF
                     GIALHVLAYEEGMLGLPRVARVQRLAAVRPGRARDAFLRLDADR"
     gene            complement(2939..4141)
                     /locus_tag="AC16HB_16580"
     CDS             complement(2939..4141)
                     /locus_tag="AC16HB_16580"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5608458.1"
                     /translation="MFIDRVLSGMRPTGALHIGHYHGALKNWVRLQDEHPCFFFVADW
                     HALTTHYESPEVLEDSVWDMLVDWFAAGIDPQKATVFIQSRVPEHSELFLLLAMATPL
                     GWLERVPTYKDQIEKLKERDLSTYGFLGYPLMQAADILIYRANMVPVGEDQVPHIEMT
                     REIARRFNHLFGSEPGFEEKANAAVKKLGTKRAKLYKDLRAKYQEQGEEDALEAARAL
                     LADAQSLSMGDRERLFGYLEGARRVILTEPDALLTEVPKLPGLDGQKMSKSGGNAVLM
                     REEPKVLAQKIRTMPTDPARVRRTDPGDPEKCPVWQLHLVYSNDDTRAWVQKGCRSAG
                     IGCLECKQPVIEAMVREQEPWRQRAQPYLDDPSLLRSIVADGCDRARTVAQETMRDVR
                     EAMGLAYT"
     gene            complement(4144..4791)
                     /locus_tag="AC16HB_16585"
     CDS             complement(4144..4791)
                     /locus_tag="AC16HB_16585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5608459.1"
                     /translation="MEGLIQTISVYAIPVLLAITLHEAAHGYVARVFGDTTAYAAGRV
                     TLNPLKHIDPIGTIVVPVAILLLAKLAGGSGFLFGWAKPVPVNFARLRNPKQDMVWVA
                     GAGPGSNLLMAVGWALFLKLQFALGLHEEFFAEMSKAGILVNIGLMALNLLPVPPLDG
                     GRIAVGLLPHQLAYQFARIEPYGLFIVLALLATNTLGFFLEPFYHFGLAVVELFL"
     gene            complement(4806..5432)
                     /locus_tag="AC16HB_16590"
     CDS             complement(4806..5432)
                     /locus_tag="AC16HB_16590"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5608460.1"
                     /translation="MAQLFSVHPLNPQVRLLRQSAQLIDHGAVAAIPTDSSYALVCHL
                     DDKPAADRLRRIRHVDERHHLTLLCRDLSEIANYAKIDNVQYRLLKQVTPGPYTFILE
                     ATREVPRRLSHPSRKTIGIRVPGHPVALGLLDVLGQPLISTTLLLPGDDMPLNDAQMI
                     LERIGHDLDIVIDAGACALEPTTVVDLTGREAVLIRRGRGPIEALGLR"
     gene            complement(5441..6292)
                     /locus_tag="AC16HB_16595"
     CDS             complement(5441..6292)
                     /locus_tag="AC16HB_16595"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HIR5608461.1"
                     /translation="MPHALHSLNADLHAHSVASDGSLSPRALVRRARERRLDLFALTD
                     HDTIDGLAEAQAAAEEFGLRFVAGVEVSVTWAGETVHVVGLGIDPGNATLGAGLAEVR
                     AGRLQRGREMGEQLAQAGIEGAFEGALALAGNPDLLSRTHFARYLVQTGFCAGTREVF
                     RRFLVAGKPGYVPHRWAALADAVRWIRAAGGAAVLAHPGRYKVDDMQLDALLSEFKAL
                     GGAAIEVSTSNHGAHEAKRFAQHAQRFGFEASRGSDFHSPADADVELGRVDALPAALV
                     PVWHRYA"
     gene            complement(6334..7203)
                     /locus_tag="AC16HB_16600"
     CDS             complement(6334..7203)
                     /locus_tag="AC16HB_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5608462.1"
                     /translation="MKPSRSRFIELRRLRYHVREWGRDGAPKLVLLHGWMDVSASFQF
                     VVDELKRDWHVLAPDWRGYGLTAWTAADCYWFADYLADLDFLLDALAPGETVRIAGHS
                     MGGNVALMYAGIRPARVAAVVNLEGFGLKGATPDMAPDRYARWIDELKAPATLRDYGS
                     LGEVAERLQRNNPRLAAEAAMFLAAHWSQPTPDGRWRIAGDPAHKIVNPVLYRWEEVA
                     ACWARIKCPVLWVQAAHTDAHQWAGGQAEIDRRSRAVPNVQYATVADAGHMLHHDQPR
                     AVAQLIERHFAPA"
     gene            7265..8935
                     /locus_tag="AC16HB_16605"
     CDS             7265..8935
                     /locus_tag="AC16HB_16605"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR5608463.1"
                     /translation="MPKSRPDQYRALYEAFRWHVPREFNIAEVCARRWAQETSRVAIH
                     YEDERGTRAVYTYAALQSDANRLSNALAGLGVGRGDRVAILLPQRPETAIAHIALYQM
                     GAIAMPLSILFGPDALEYRLQDSGAAAALCDDAALEALTMARATCPQLAHVITAGIKA
                     PGTHHWGELLARASDRFEPVSTLASDPALLIYTSGTTGPPKGALMPHSALIGNLPGFV
                     CSQDWFPRPGDVFWSPADWAWTGGLMDALLPTLYFGFPIVGFNGRFAPERALDLMQRY
                     RVTNTFLFPTALKMMMRSPAWRGVRASLALRAIMSAGESVGTTVFDWTQDELGITVNE
                     MFGQTEMNYIVGNSQARWPARPGAMGRPYPGHRVEVIDDEGRALPPGATGEVAVHRRD
                     IHGHPDPVFFLEYWKNPEATRRKFTGPSVDSWCRTGDLASRDEQGYLWYQGRADDMFK
                     AAGYRIGPSEIENCLLKHSAVVNVAVVPKPDAERGAVVKAFVVLAPGAVGGARLIADL
                     QAHVRGKLAPYEYPKEIEFIEQLPMTTTGKVQRRVLRELEARRASGER"
     gene            complement(8969..9304)
                     /locus_tag="AC16HB_16610"
     CDS             complement(8969..9304)
                     /locus_tag="AC16HB_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608464.1"
                     /translation="MGVLDGIRTLFNVRVMRARGERGPKPRLGAKICLGDVRISVQSG
                     LTDDMWSWLQEQGWREITHRPDRRRYRDVPPSLVTKLFDAVPTDRIALLKEAMHAAVA
                     KPPAQLGRG"
     gene            complement(9383..>9872)
                     /locus_tag="AC16HB_16615"
     CDS             complement(9383..>9872)
                     /locus_tag="AC16HB_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR5608465.1"
                     /translation="VAAEEGRHFALLCGRLAACGARYGDFDAHDGLWDMAYKTRHDPL
                     ARMALVPRVLEARGLDASPLVRAKLARAGDRDSAAVIDVILRDEIGHVALGNRWFHFL
                     CAAQGRTPAAVDLAVRAEYAAPLPRPPFNFAARRAAGFDADELSALDAGRPLAGRTRE
                     RR"
BASE COUNT         1579 a         3413 c         3325 g         1555 t
ORIGIN      
        1 tgatccgaat gctcgtgcgt gacgacgacc gcatccagat cgtcgattgc catggacgcg
       61 cgacccagcc gctttgccag cgcgcgcggc ccgaatccgt tgtcgaccag caggcgcgtg
      121 acggtcagcc cgtcatgcac ctcgaccacc agcgcgttgc cctcgctgcc cgagccgaga
      181 ctgcagaacc gcagcacggc ggcgcgccgc tactttagtt gctcgttgat ctggcccagg
      241 atcttctgcg ccgtcgcgcc accgtcgggc ttgccttcct tgtcgagcac ggtcacggac
      301 gtcgcgtcgc ccgctgcggc cacctgcacg cggaactgct gcggatcgat cttggggtcg
      361 cgcccgaaca tgcgtgagaa gaagccctgg ttctcgcgct gcttggcctc gaactccggg
      421 tccaggtaac gcacgaagta cgtgcccttc gtgcggtcgc gatcctcgac ggtgaagccg
      481 ccacgatcga gcgccagccc gacgcgccgc cacgcgcgat cgaacggctc gtcgatctgc
      541 acacgctgtg ccttgccgtc ggcgccgcgc acgatgcgcg cgatctcatg cgccgccgct
      601 gctgcggccg gggcagttgc ggcagtcgcc ggagccgccg tggccgtcgt cgtcgcgggc
      661 aggaagcgca gcagcagtcg ctgcagcatc tcggcctcga gttcgtgatc cggctcgcgc
      721 ggctgccagc gcgtctggtc cctttgcgtg ctcgaataga cctcgaccat gccgcgatgg
      781 ctgatgtaga cctcggcggt gttcttcggt gtgcgctcga tgcgcgtgcg gaacttgtcc
      841 tgctcgccgg tcgaatacag gccgtcgatc aggcgaccga tcgtgcggcg gatgatgtcc
      901 tgcggtagct tggcgcgatt ttccgcccac gtggtctcga cgattccggc cgccgcatcc
      961 tcgcgctcga tcacgaggcc gacgctcggc cagaattcct tgaccacgac gaacgcctgc
     1021 tcgggagtca tgtcgaccgc cagccagcgc tcggcgccgt cgcgctcgat gcgcgcgcgc
     1081 gccgtcgccg gcgcgacggt ctgcactgcc gccgcgggtg cgcgcgccgc gccgatcgcg
     1141 ctggcgctcg ccacgccggg gcgatcggaa agcgcgtagc ggtcgtcgcg cggaatctgc
     1201 gacaggtcgg gcggcacttc gagcgacttg gtcggcgcca ccgacttgta gtcgatcttg
     1261 tcggtcgaca gcccgagcga actgcagccc gcgagcgccg cagccagcac gaataggccg
     1321 gcgcaacgca ccgcctgcgt cacattcaac atccacagtc tccctgagat cggatcaggc
     1381 gatgcccgct tcctgcagcg ccgccgcgac catgggctgg ttggtcgccg acagcggcac
     1441 cagcggcagc ctcacgtgcg cgttgcaacg cccgagcttc gacagcgccc acttgaccgg
     1501 ccccggattg ctttcgacaa agagctttgc atgcagcggc agcagacggt tgttgagcgc
     1561 gcgcgcggtt gcgagttcgc ccgcgagcgc cgcgcgacac atgtcgctca tcggccgcgg
     1621 cgccacgttc gcggtgaccg agatcacgcc gtggccgccg agcagcagca gcgccagcgc
     1681 gctgtcgtcg ttgccgctgt acaccgcgaa cgatctgggc agccgccgca gcaggtccgc
     1741 ggcgcgcgcg aggtcgccgg tcgcatcctt cagcgccacg atgcccggga tctgcgcaag
     1801 tcgcacggtg gtgtcgttgg ccagatcggc gaccgtacgg cccggcacgt tgtacaggat
     1861 caccggcaag tcgaccgtct cggcgacctt gcggaaatgc tggtacaggc cttcctgcgt
     1921 cggcttgttg tagtaaggca cgacctgcag cgaggcatcc gcgccgacct tctttgcata
     1981 ctgggtgagg tcgatcgcct cgctggtcga attgccgccg gtgcctgcga tcaccggcac
     2041 gcgccggcgc accgtctcga ccgcgatgcg gatcagttca caatgttcgt cgtgatcgac
     2101 ggtcggcgat tcgccggtgg tgccgaccgc aacgatgccg ttcgtgccct cggcgatctg
     2161 ccattcgatc agccgccgat aggcgtcgta gtcgagcgcg ccgtcctcgc gcatcggggt
     2221 gacgatcgcc accaggctgc cagtgatcat gtgttcctcg caggaaacgg atcgcgcgat
     2281 tctaccgttg ccgcgtcgcg cgagcgggct tcagcgatcg gcgtcgaggc gcaggaaagc
     2341 atcgcgtgcg cggccgggcc gcaccgcagc cagccgctgc acgcgcgcca cgcggggcag
     2401 gccgagcatg ccctcctcgt acgcgagcac atgcagtgcg atgccgaata cgtcgagcag
     2461 ttcgcgcggt ttcagcagaa acgccggatt gctcggcttg ccgtagcgct cgttgccgac
     2521 cgcgaaggtt tcgtacagca gcaccccgcc gggcgcgagc gagtccgcca acaccgggaa
     2581 cagcgcccgg tacaggtagt tggtgacgac gatcgcgtcg aattggcggc cgcgatacgg
     2641 ccacggtccg ttctccaggt ccgcgcgccg gaacgcgacc tgcggcgcct ccctgaaggc
     2701 gtccgcgcag gcgggatcga tgtcgaccgc gtcgaccagg cagcgctgcg cagcgaacag
     2761 cagcgcatgc cgaccgtgtc cacaggccag atcgagcacg cggctgcccg gcggtaccag
     2821 cggcgcgaaa cgctccaccc aggccgacgg ctccgcgccc tcatgcgcag cgccgaccaa
     2881 ggcgttgcgc acggctgcag tcgcgcgggc gcgcggatcg atcatcgggg tgcggcgctc
     2941 aggtgtacgc gagacccatc gcctcgcgca cgtcgcgcat cgtttcctgc gcgacggtgc
     3001 gcgcccgatc acaaccgtcg gcaacgatcg aacgcagcag cgacgggtcg tccaggtatg
     3061 gctgcgcgcg ctggcgccag ggttcctgct cgcgcaccat cgcttcgatc accggctgct
     3121 tgcactcgag gcagccgatg ccggcgctgc ggcagccctt ctgcacccac gcgcgcgtgt
     3181 cgtcgttcga atagacgagg tgcagctgcc acaccgggca tttctcggga tcgccgggat
     3241 cggtgcgacg cacgcgcgcc ggatcggtcg gcatcgtgcg gatcttctgc gccagcacct
     3301 tcggctcctc gcgcatcagc accgcgttgc cgccactctt cgacatcttc tgtccgtcga
     3361 gtcccggcag cttcggcacc tcggtcagca gcgcatccgg ctcggtcagg atcacgcggc
     3421 gcgcgccttc cagatagccg aacagccgct cgcggtcgcc catcgacagg ctctgcgcat
     3481 cggcgagcag cgcccgcgcc gcttcgagcg catcctcttc gccctgctcc tggtacttcg
     3541 cacgcaaatc cttgtacagc ttggcacgct tggtgccgag cttcttgacc gccgcgttcg
     3601 ccttctcctc gaaacccggc tcactgccga acaggtggtt gaagcggcgc gcgatctcgc
     3661 gcgtcatctc gatgtgtggc acctgatcct cgccgaccgg caccatgttg gcgcggtaga
     3721 tcaggatgtc ggcggcctgc atcagcggat aaccgaggaa gccgtaggtg gacagatcgc
     3781 gctccttcag cttctcgatc tgatccttgt aagtcggcac gcgctccagc caaccgaggg
     3841 gggtggccat cgccagcagc agaaacagtt cggagtgctc gggaacgcgc gactggatga
     3901 acaccgtggc cttctgcgga tcgatgccgg cggcgaacca gtcgaccagc atgtcccaca
     3961 cgctgtcttc gagcacctcg ggcgactcgt agtgcgtggt caacgcgtgc cagtcggcga
     4021 cgaagaagaa gcacgggtgc tcgtcctgca gccgcaccca gttcttgagc gcgccgtgat
     4081 agtggccgat gtgcagcgca ccggtcgggc gcatgccgga aaggacgcga tcgatgaaca
     4141 tgatcagaga aacagttcca cgaccgccag gccgaagtga tagaagggct ccaggaagaa
     4201 cccgagcgta ttggtcgcca gcagcgcgag cacgatgaag aggccgtacg gctcgatgcg
     4261 cgcgaactgg tatgcaagct ggtgcggcag caggccgacc gcgatgcgcc cgccgtcgag
     4321 cggcggcacc ggcagcaggt tcagcgccat cagaccgatg ttgacgagga tgccggcctt
     4381 cgacatctcg gcgaagaact cctcgtgcaa accgagcgcg aactgcaatt tcaggaacag
     4441 cgcccagccc accgccatca gcaggttcga tccgggtccc gcgccggcga cccagaccat
     4501 gtcctgcttc ggattgcgca ggcgcgcgaa attcactggg accggtttcg cccatccaaa
     4561 cagaaatccg gacccgccag cgagcttggc cagcagcagg atcgccaccg gcacgacgat
     4621 ggttccaatt ggatcgatgt gtttcaacgg gttgagcgtg acgcgtcccg ccgcgtacgc
     4681 ggtcgtgtcg ccgaacacgc gtgccacgta gccgtgcgcg gcttcgtgca gggtgatcgc
     4741 gagcaggacg gggattgcgt agaccgagat ggtctggatg aggccttcca tcgcggcaga
     4801 gtctatcagc gcagtccgag tgcctcgatc ggaccgcggc cgcgccggat cagcaccgct
     4861 tccctgcccg tcaggtcgac gacggtggtt ggttcgagag cgcaggcgcc ggcatcgatc
     4921 acgatgtcga ggtcgtggcc gatccgctcg aggatcatct gcgcgtcgtt cagcggcatg
     4981 tcgtcgcccg gcagcagcag tgtcgtcgag atcagcggct ggccgagcac gtcgagcagc
     5041 ccgagcgcaa ccggatgccc gggcacgcga atgccgatcg tcttgcgcga cgggtgcgac
     5101 aggcgacgcg gcacctcgcg cgtggcttcg agaatgaatg tgtacggccc cggcgtcacc
     5161 tgcttgagca gccggtattg cacgttgtcg atcttcgcgt aattggcgat ttccgagagg
     5221 tcgcggcaga gcagcgtgag gtgatggcgc tcgtcgacat ggcggatgcg ccgcaatcgg
     5281 tccgccgccg gcttgtcgtc gaggtggcac accagcgcat aggacgagtc ggtcggaatc
     5341 gccgcgaccg cgccatgatc gatcagctgc gcggactgcc gcagcaaccg aacctgcggg
     5401 ttcagcgggt gtaccgagaa aagctgcgcc atcgcgacgg tcaggcgtat cgatgccaga
     5461 ccggcacgag cgctgcaggc aacgcatcga cgcgaccgag ctccacgtcc gcatcggccg
     5521 gactgtggaa gtcgctgccg cgcgatgctt cgaagccgaa ccgctgcgcg tgctgggcga
     5581 agcgcttcgc ttcgtgcgcg ccgtgattgc tggtcgagac ctcgatcgca gcgccgccga
     5641 gcgccttgaa ttccgacagc agtgcgtcga gctgcatgtc gtcgaccttg tagcggccgg
     5701 gatgagcgag cactgcggcg ccgcctgctg cgcgaatcca gcgcaccgcg tcggcaagcg
     5761 cggcccagcg atgcggcacg tatccgggct tgcccgcgac caggaagcga cggaacacct
     5821 cgcgcgtgcc ggcacagaaa ccggtctgca ccagataccg cgcgaaatgc gtgcgcgaca
     5881 gcaggtccgg attgccggcc agcgcgagtg cgccctcgaa cgcgccttcg atgccggcct
     5941 gcgcgagctg ttctcccatc tcgcgcccac gctgcagtcg acccgcgcgc acttcggcca
     6001 gcccggcgcc caacgtggcg ttgcccggat cgatgccgag gccgacgacg tgcaccgttt
     6061 ctcccgccca ggtgacggac acctcgacac cggcgacgaa gcgcaagccg aactcttccg
     6121 ccgccgcctg cgcctcggcg aggccgtcga tggtgtcgtg atcggtcagc gcgaacaggt
     6181 ccaagcgccg ctcgcgcgcg cgccggacaa gggcacgtgg tgagagcgac ccgtcggagg
     6241 ccaccgagtg cgcgtgcaga tcggcgttga gcgaatgcag tgcgtgcggc atggcgccat
     6301 tgtaggtggc gcgcacatcc gatccggcgc cggtcacgcg ggcgcgaagt gccgctcgat
     6361 cagttgcgcc accgcacgcg gttgatcgtg gtgaagcatg tggcccgcat ccgccaccgt
     6421 cgcgtactgc acgttcggca cggcgcggct gcggcgatcg atctccgcct ggccgcccgc
     6481 ccattgatgc gcgtcggtgt gtgcggcctg cacccacagc accgggcact tgatgcgcgc
     6541 ccagcaggcc gcgacctcct cccagcggta caggacggga ttgacgatct tgtgcgccgg
     6601 atcgccggcg atgcgccagc gcccgtccgg tgtcggctgt gaccagtgcg cggcgaggaa
     6661 catcgccgct tccgccgcca gtcgcggatt gttccgctgc agccgctccg cgacttcgcc
     6721 gagcgagccg taatcgcgca gcgtcgccgg cgccttcagc tcgtcgatcc agcgcgcata
     6781 acggtccggc gccatgtcgg gtgtcgcgcc cttcagcccg aaaccttcga ggttgacgac
     6841 cgctgcgacc cgcgccggcc ggatgccggc gtacatcagc gcgacgttgc cgcccatgct
     6901 gtggcctgcg atgcgcactg tttcgcccgg cgcgagcgcg tcgagcagga agtcgaggtc
     6961 ggccaggtaa tcggcgaacc agtagcagtc ggccgccgtc cacgcggtca ggccgtagcc
     7021 gcgccagtcg ggcgcgagca cgtgccagtc gcgcttgagt tcgtcgacga cgaactggaa
     7081 cgacgccgaa acgtccatcc agccgtgcag cagcacaagc ttcggcgcgc cgtcgcggcc
     7141 ccattcgcgc acgtggtagc gcagcctgcg tagttcgatg aagcgggaac gcgaaggttt
     7201 catgttgccc ggcggagcac cgttggagga tgatcggcgc caccacgata ccaagctcac
     7261 gatcatgccg aagtcccgac ccgatcagta ccgcgcgttg tacgaggcat tccgctggca
     7321 cgtgccccgt gagttcaaca tcgcggaggt gtgcgcgcgc cgctgggcgc aggagacctc
     7381 gcgcgtggcg atccattacg aggacgagcg cggcacacgc gcggtctaca cctacgcagc
     7441 gctgcagtcc gacgcaaacc ggctgtcgaa cgcgctggcc gggctcggcg tcgggcgcgg
     7501 cgatcgcgtt gcgatcctgt tgccgcaacg accggagacg gcgattgcgc acatcgcgct
     7561 ttaccagatg ggcgcgatcg cgatgccgct gtcgatcctg ttcggccccg acgcgctcga
     7621 gtaccggctg caggacagcg gcgctgcggc cgcgctctgc gacgacgcgg cgctcgaggc
     7681 actgacgatg gcgcgggcga cgtgcccgca actcgcgcac gtgatcaccg ccggcataaa
     7741 ggcgccgggt acgcatcact ggggcgaact gctggcgcgc gcgtcggatc gcttcgaacc
     7801 cgtgtcgact ctggccagcg acccggcgtt gctgatctac accagcggca ccaccggacc
     7861 gcccaagggc gcgctgatgc cgcacagcgc gctgatcggc aacctgcccg gcttcgtctg
     7921 ctcgcaggac tggtttccgc ggccgggcga cgtgttctgg tcgccggccg actgggcgtg
     7981 gaccggcgga ctgatggacg cgctgctgcc gacgctgtac ttcggctttc cgatcgtcgg
     8041 cttcaatggc cgtttcgcac cggagcgcgc gctggacctg atgcagcggt atcgcgtcac
     8101 caatacgttc ctgttcccga cggcgctgaa gatgatgatg cgctcgcccg cctggagggg
     8161 cgtgcgcgcg tcgcttgcgc tgcgcgcaat catgagcgcg ggtgaatcgg tcgggaccac
     8221 ggtgttcgac tggacgcagg acgaactcgg catcacggtg aacgagatgt tcggccagac
     8281 cgagatgaac tacatcgtcg gcaactcgca ggcgcgctgg cccgctcggc ccggcgcgat
     8341 gggtcggcca tacccggggc atcgcgtaga ggtaatcgac gacgaagggc gcgcactgcc
     8401 gcccggcgca accggcgagg tcgcggtcca tcggcgcgac atccatggcc atcccgatcc
     8461 ggtcttcttc ctcgagtact ggaagaaccc ggaggcgacg cggcgcaaat tcaccggccc
     8521 gtcagtcgac tcgtggtgcc gcaccggcga cctcgcgtca cgcgacgagc agggctacct
     8581 ctggtaccag ggccgcgccg acgacatgtt caaggccgcc ggctatcgga tcgggccgag
     8641 cgaaatcgag aactgcctgc tcaagcattc cgcggtggtc aacgtcgcag tcgtcccgaa
     8701 gcccgatgcc gagcgcggcg cagtcgtcaa ggcattcgtc gtgcttgctc ccggtgcggt
     8761 cggcggtgcg cggctgatcg ccgacctgca ggcgcacgtg cggggcaagc tcgcgcccta
     8821 cgagtacccg aaggagatcg aattcatcga gcagttgccg atgacgacca ccggcaaggt
     8881 gcagcggcgt gtgctgcgcg aactcgaggc acgccgcgcg agcggggaac gctgaactac
     8941 tgcgcgcggg aactggacga tgtccggctc aaccgcggcc gagctgggcc ggaggcttgg
     9001 cgaccgcggc atgcatcgct tccttcagca gcgcgatgcg gtcggtcgga acggcatcga
     9061 acagcttggt gaccagcgac ggcggcacgt cgcgatagcg gcgccggtcg ggacggtgcg
     9121 tgatctcgcg ccagccctgc tcctgcagcc agctccacat gtcgtcggtc aggccggact
     9181 gcacgctgat acggacatcc ccgagacaga tcttcgcgcc cagccgcggc ttggggccac
     9241 gttcgccgcg cgcccgcatc acgcgcacgt tgaacagcgt tcggataccg tcgagtacac
     9301 ccatatctga atcagtacct ttgcagcgcg atcttcaatg cacttcgagc ggaggcaaat
     9361 gtagccttca tcggccccgg gctcaacgcc tctcgcgcgt gcggcctgcc aatggccgac
     9421 cagcgtcgag cgccgacagt tcgtcggcgt cgaagccggc cgctcgtcgg gcggcgaaat
     9481 tgaatggcgg gcgcggaagc ggcgcagcgt actcggcgcg caccgcaagg tcgaccgccg
     9541 caggagtgcg tccctgcgcc gcgcacagga agtgaaacca gcggttgccg agcgcgacgt
     9601 ggccgatctc gtcgcgcagg atcacgtcga tcacggcggc cgaatcccga tccccggcgc
     9661 gcgcgagctt cgcgcgcacg agcggcgagg cgtcgagccc gcgtgcctcg agcacgcgcg
     9721 gaaccagcgc catgcgcgcc agtgggtcgt gccgcgtctt gtacgccatg tcccacagcc
     9781 cgtcatgggc gtcgaagtcg ccgtagcgcg ccccgcacgc cgccagccgg ccgcacagca
     9841 gcgcgaagtg acgcccctcc tcggccgcga cc
//
LOCUS       DBNHBY010000700         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_593512, whole genome shotgun sequence.
ACCESSION   DBNHBY010000700 DBNHBY010000000
VERSION     DBNHBY010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_593512"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16HB_16620"
     CDS             <1..270
                     /locus_tag="AC16HB_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR5608466.1"
                     /translation="GTISIADDGTVTGSVTTKGIQGTMAHIHLGAADKNGPVVVPFTK
                     DGDTYKAPAGAKLNADQMKDFKAGDLYFNVHSAAHPGGEIRGQLK"
     gene            complement(292..1053)
                     /locus_tag="AC16HB_16625"
     CDS             complement(292..1053)
                     /locus_tag="AC16HB_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5608467.1"
                     /translation="MRFGFPLRQWLPLALLALVHGVACAADIAVFSTPTFKSALQALA
                     PAFERASGHRLVVTYDSVAALKRRIEAGESFEAALLLPDAIDALVKAGRIDPSSVTPL
                     ARTAVGVAIHAGLPVPDVGSIDALKSALLQARSLAYGPDSASGSYFVKLLDRLGIPDA
                     RAKLVPVAGGHVMDAVARGDAEMTVITVPNIVGVPGVTLAGKLPEPLQHYTTFTGGVS
                     AAAAPASAARALLKFLTTDEAAAAFERFGLDRPAP"
     gene            1172..2101
                     /locus_tag="AC16HB_16630"
     CDS             1172..2101
                     /locus_tag="AC16HB_16630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5608468.1"
                     /translation="MARSEHLRRRLHLRDLDTFMTVAASGGMRKAAEQLHMSQPAVSK
                     AIADLEGLLGARLFDRSRSGVVPTAYGRALLKHAATLFDGLRRAVGEIEFLADPAGGE
                     LSLGCAETISAGLAAAVIASISKRQPRVVFNVESGDAPVLLLHFLRERTCELVIARPY
                     GAVDPDVDALPLFRERMTVVVGPTHRFASRRKIRLEELIDERWLLSRNELMADSPVAA
                     AFAGAGLSVPSKLIVTGSLNLRYALLATGSCVTVVPHSLLKFGTPRGALRLLPLELPA
                     WSQPTCILSLKGRVMSPIARMFVDTTLELTRGM"
     gene            complement(2114..>3146)
                     /locus_tag="AC16HB_16635"
     CDS             complement(2114..>3146)
                     /locus_tag="AC16HB_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5608469.1"
                     /translation="DADFTGSDGNGIGANFSRRSCILSAMGPRTDWTEERELVARALA
                     GDGASLATAFERWRPRLLAAALRLLADRAEAQDAVQETFLVALAHLPELRAQPAFGGW
                     LLAILRNHCLQQMRSRRHEERVEEIADDALIDHGIEHRLDRHALRDWILGTIGALPEP
                     FKLAALLRYFGSYPSYAEIAAILDVPVGTVRSRLAEVKQRLADAMLAQAGLPTENDDD
                     HVAARLAEYTDAFGGLEWQRRQRLISRCTEHVQLVWARPGHAPTWLRGRAHLEADLRD
                     DLAHGVRYGLTRALGDAQVSVFETEFHNDPQFPDHCPPGATVLMVHDGAGIERLHVYL
                     SPRPPSEAP"
BASE COUNT          496 a         1084 c         1093 g          473 t
ORIGIN      
        1 ggcacgatct ccatcgccga cgacggtacg gtcaccggca gcgtcacgac caagggcatt
       61 cagggcacga tggcgcacat tcacctcggc gcggccgaca agaacggccc cgtcgtcgtt
      121 ccgttcacga aggacggcga cacgtacaag gcgccagccg gcgccaagct caatgccgat
      181 caaatgaaag acttcaaggc cggcgatttg tacttcaacg tgcacagcgc cgcgcacccc
      241 ggcggcgaga ttcgcggcca gttgaaatag cgcctgacca aaggcaccgg ttcacggcgc
      301 cgggcggtcg agtccgaatc gctcgaacgc agcggcggcc tcgtctgtcg tcaggaactt
      361 cagcaacgcg cgcgcggcgg acgcgggcgc tgccgccgcg gacacgccgc cggtgaaggt
      421 cgtgtagtgc tgcagcggct cggggagctt gcccgccagg gtgacgccgg gtacgccgac
      481 gatgttgggc accgtgatga ccgtcatctc ggcgtcgccc ctggcgacag cgtccatcac
      541 gtgaccgccg gccacgggca cgagcttcgc gcgcgcgtcg gggatgccca ggcgatcgag
      601 cagtttcacg aagtaactgc cgctggcgct gtcgggtccg taggccaggg agcgtgcctg
      661 cagcagcgct gacttgagcg catcgatcga cccgacgtcc ggaaccggca gccccgcgtg
      721 gatcgcgacg ccgacggcag tgcgcgccaa gggcgtgacg gacgacgggt cgatccgccc
      781 cgccttcacc agtgcgtcga tcgcgtccgg cagcagcagc gcggcctcga acgactcgcc
      841 ggcctcgatc cgccgcttca gcgcggcgac gctgtcgtag gtgaccacca gccggtgccc
      901 gctggcgcgt tcgaacgccg gcgccagtgc ctgcagcgcc gacttgaacg tcggcgtgct
      961 gaagacggcg atgtcggctg cacaggccac gccgtgaacg agcgccagca gcgcgagcgg
     1021 cagccactgc cgcaggggaa agccgaacct catgatgtgc gactccttcg cattcggatg
     1081 gcaagggacc aaacggcgcg ggagcctacg agttgcgaag atcaaagaaa agctcgattt
     1141 cgtgatcccg cgatcaataa tccggatcac catggctcga agcgaacacc tgcggcggcg
     1201 gctgcacctg cgcgatctcg acacgttcat gacggtcgcc gcgtcgggtg gaatgcgcaa
     1261 ggcggccgag caactgcaca tgtcgcagcc ggccgtgtcg aaggccatcg ccgatctgga
     1321 aggcctgctg ggcgcgcgcc tgttcgaccg cagtcgcagc ggcgtggtac caactgccta
     1381 cggtcgcgcg ctgctgaagc acgccgcgac gctgttcgac ggactgcgcc gcgccgtcgg
     1441 cgagatcgag ttcctcgccg accccgccgg cggcgaactg agcctggggt gcgcggaaac
     1501 gatctccgcc gggctggcgg ctgcggtaat cgcgagcatc tcgaagcggc agccgcgggt
     1561 cgtgttcaac gtcgagtcgg gcgatgcgcc ggtgctgctg ctgcacttcc tgcgcgagcg
     1621 cacctgcgaa ctcgtcatcg cgcggccgta cggcgcggtc gatcccgacg tcgacgcgtt
     1681 gccgctgttc cgcgaacgca tgacggtggt cgtcgggccg acgcaccgct tcgcgtcgcg
     1741 ccgcaagatc cggctcgagg aactgatcga tgagcgttgg ctgctttcgc gcaacgagct
     1801 gatggccgac tctccggtgg ccgcggcctt cgcgggcgcg gggctgtccg tgccgagcaa
     1861 gctcatcgtc accggctcgc tgaacctgcg ctacgcgctg ctcgcgaccg gatcgtgcgt
     1921 gacggtcgtg ccgcattcgt tgctgaagtt cggcacgccg cgcggcgcct tgcggttgct
     1981 gccgctcgag ctgccggcgt ggagtcagcc gacctgcatc ctgtcgctga aaggccgggt
     2041 gatgagtccg atcgcgcgga tgttcgtcga caccacgctg gaactgacgc gcggcatgtg
     2101 atgcgcgcgc tcgtcagggc gcttcgcttg gcggccgcgg cgacaggtag acgtgcagtc
     2161 gctcgatccc ggcgccgtcg tgcaccatga gcacggttgc gccgggcggg caatggtccg
     2221 gaaactgcgg atcgttgtga aactcggtct cgaacacgct gacctgcgca tcgccaagcg
     2281 cgcgcgtcag accgtagcgc acgccgtgcg ccaggtcgtc gcgcagatcg gcctccaggt
     2341 gcgcgcggcc gcgcagccac gtcggggcat gcccggggcg cgcccacacg agctgaacgt
     2401 gttcggtaca gcgggagatc aaacgctgcc ggcgctgcca ctcgagcccg ccgaaggcat
     2461 cggtgtattc ggccagccgc gcggcgacgt ggtcgtcatc gttttcagtc ggcagtcccg
     2521 cctgcgccag catcgcgtcg gcgagccgct gcttgacttc cgccagccgg ctgcgcacgg
     2581 tgccgaccgg cacgtcgagg atcgcggcga tctccgcgta cgacggatag ctgccgaaat
     2641 agcgcagcag cgcggcgagc ttgaacggct ccggcagcgc gccgatcgtg ccgaggatcc
     2701 agtcgcgcag cgcgtgccgg tcgagccggt gctcgatgcc gtggtcgatc agcgcatcgt
     2761 cggcaatctc ctctacgcgc tcctcgtgcc tgcggctgcg catttgctgc aggcagtggt
     2821 tgcgcaggat cgccagcagc cagccgccga acgccggctg cgcgcgcaac tccggcaggt
     2881 gcgcgagcgc gaccaggaac gtctcctgca cggcatcctg cgcttcggcg cggtcggcga
     2941 gcaggcgcag agccgccgcc agcagccgcg gccgccagcg ctcgaacgcg gtggcgaggc
     3001 tcgcgccgtc acccgccaac gcgcgtgcca ccagttcgcg ttcctcggtc cagtcggtgc
     3061 gaggtcccat cgctgacagg atgcacgagc gccgcgaaaa gttcgcgccg attccgttgc
     3121 cgtccgatcc cgtaaagtcg gcatcg
//
LOCUS       DBNHBY010000701         5908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1677538, whole genome shotgun sequence.
ACCESSION   DBNHBY010000701 DBNHBY010000000
VERSION     DBNHBY010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1677538"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..882)
                     /gene="phbB"
                     /locus_tag="AC16HB_16640"
     CDS             complement(145..882)
                     /gene="phbB"
                     /locus_tag="AC16HB_16640"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR5608470.1"
                     /translation="MAQKVAYVTGGMGGIGTSICQRLHKDGFKVIAGCGPTRDYNKWL
                     GEQKALGYTFFSSVGNVADWNSTVEAFAKAKAEHGPIDVLVNNAGITRDRLFVKMTRE
                     DWDAVIDTNLNSLFNATKQVVADMVERGWGRIIQISSVNGEKGQAGQTNYSAAKAGMH
                     GFTMALAQEMASKGVTVNTVSPGYIGTDMVRAIKPEILEKIVGTIPVKRLGTPDEIGS
                     IVAWLAGPDSGFTTGADFSCNGGLHMG"
     gene            complement(930..2108)
                     /locus_tag="AC16HB_16645"
     CDS             complement(930..2108)
                     /locus_tag="AC16HB_16645"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5608471.1"
                     /translation="MSKAVIVAAARTAVGKFGGALAKTSAADLGAIVIKEVLARAKLP
                     PERVEEVIMGQVLAAGCGQNPARQALIKAGLPVAVPAMTINKVCGSGLKAVMLAAQAI
                     ANGDAEVVVAGGQENMSLAPHVLMNSRDGFRMGDAKMIDSMIVDGLWDVYNKYHMGIT
                     AENVAKQYAIDRAQQDAFALASQQKAAAAQDAGRFKEEIVAVMIPQRKGDPVAFDTDE
                     FINRKSNAETLAGLKPAFDKEGTVTAGNASGINDGAAAVVVMSEARAAALGLTPLARI
                     ASFVSAGVDPKIMGMGPAPASRKALEKAGWKAADLDLMEINEAFAAQACAVNKEMGWD
                     TGRINVNGGAIAIGHPIGASGCRILVTLLHEMKRRGSKRGLASLCIGGGMGVALAVER
                     "
     gene            complement(2118..3833)
                     /locus_tag="AC16HB_16650"
     CDS             complement(2118..3833)
                     /locus_tag="AC16HB_16650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR5608472.1"
                     /translation="MAGSLRGAEAHANDNPWLNWWSSLAAMAPTGADVPRIGLAPERV
                     LQLQQEYVQRMGRLWSDFMEHPDKSSEPIRDNRFADPAWQQNPLASFYARTYLLNSEF
                     MNALADSVDADRKTKRRVKFAVSQWVDAMSPANFLALNPKAQQTLIETKGESLKAGMR
                     NLLDDIRKGKITQTDEKAFEIGRNVATSEGAVVYENELIQLIQYKPLTDKVHARPFLI
                     LPPAINKFYIMDLQPENSFVRFAVEQGNTTFLVSWRNVHEDLGHLTWDDYLEKGAIAA
                     LRAVQDICGLKGKKDNRINALGFCVGGTILASALAVLAAKGEQPAASLTLMTSLLDFE
                     DAGVLEVFIDEQHVRMRERALGKGGVMPGKELGNTFSILRANDLVWNYVVNNYLEGKQ
                     PMAFDLLYWNSDNTNLPGPMYAWYLRNMYLENNLRIPGKLKCCGKPIDLGSIKAPAYV
                     FAAVDDHIVPWKAAYASARLLTGAGEGGVRYVLGASGHIAGSINPVSKNKRSYWASTS
                     PTLPANSEAWRHGATEHQGSWWADWNRWFAQYGGGMVAAPKKYGNAQYKVIEPAPGRF
                     VKAKA"
     gene            complement(3999..4754)
                     /gene="pgeF"
                     /locus_tag="AC16HB_16655"
     CDS             complement(3999..4754)
                     /gene="pgeF"
                     /locus_tag="AC16HB_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR5608473.1"
                     /translation="MTARVHPDWIVPDWSVAARVHAFVTTRAGGVSTGPYESLNLGRS
                     TGDDLEFVNENRRVLRATVPADPRWLRQVHGAGVVDAAQIATPAEADASFTVQTGVVC
                     VVSIADCLPVFFADQSGRGVAVAHAGWRGLAAGVLQATVTALRARLGDPDARIRAWVG
                     PGIGPKHFEVGIEVLEAMECTLSRARDAFAPLADGKFRADLPALARQALAQVGVMDVG
                     GGTLCTFSDPARFFSFRRDRITGRHAAVIWREG"
     gene            complement(4751..>5908)
                     /locus_tag="AC16HB_16660"
     CDS             complement(4751..>5908)
                     /locus_tag="AC16HB_16660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5608474.1"
                     /translation="ASRVSAQPASSLAKRSRSARSRIKVSNTRESEGAAAHDYIGAAM
                     NDSARNGDLDDTEAAEALQRLTVSAALAGERVDKVLARLLPQVSRSRIQQWIEQGAVR
                     VNGAPTRARDAVLMGDVIEVAPPPEPDALAFAPEPIALDIVHEDDAIIVLDKAPGLVV
                     HPGAGNWSGTLLNGLLAHDPGLARVPRAGIVHRLDADTSGLMVVARTIEAQTDLVRQL
                     QARTVLREYWAIVIGSAPPSGTIDAAIGRDPRNPVKFKVSRAPSAREAQTRFRRIECV
                     DAGPASLSWLACRLATGRTHQIRVHLESIGHPLVGDPLYRRGRTLAQSGRDAAAWASF
                     PRQALHACRLAFKHPLRGDVREWFRPPPADMRELMQAVGFGRCDRPRDLFE"
BASE COUNT          892 a         2095 c         1839 g         1082 t
ORIGIN      
        1 gcatcaaacg acttccttgt aatcatcggc gccgcatgcg cggcgccgtc cctcggcaac
       61 agagtgtccg aaggggattc tagccacttc gcgggatggt cgactttcgc cgctgcgcca
      121 ccgcacctct gcgccccgcc aacgtcagcc catgtgcagg ccgccgttgc atgaaaagtc
      181 ggcgccggtc gtgaaacccg aatccggccc cgccagccag gcgacgatcg agccgatttc
      241 atcgggcgtc cccagccgct tgaccggaat cgtgccgacg atcttctcga gaatctccgg
      301 cttgatggcg cggaccatgt ccgtgccgat gtagccgggg ctcaccgtgt tcaccgtcac
      361 gcccttggac gccatttcct gcgcgagcgc catcgtgaag ccgtgcatgc ccgccttggc
      421 agccgagtag ttggtctggc cggcttgtcc cttttcgccg ttcaccgagc tgatctggat
      481 gatccgaccc cagccgcgct cgaccatgtc ggcgaccacc tgcttggtcg cgttgaacag
      541 cgaattcagg ttggtgtcga tcaccgcatc ccagtcctcg cgcgtcatct tgacgaacag
      601 gcggtcgcgc gtgatgccgg cgttgttgac cagcacgtcg atcgggccgt gctcggcctt
      661 ggccttggcg aatgcctcga cggtcgagtt ccagtcggcc acgttgccga ccgacgaaaa
      721 gaaggtgtag ccaagcgcct tctgttcgcc gagccacttg ttgtagtcac gcgtgggacc
      781 gcagcccgcg attaccttga atccgtcctt gtgcagccgc tggcagatcg acgtgccgat
      841 gccgcccatg cctcccgtta cgtacgccac cttctgtgcc atttgcagtc tcctctgcga
      901 ataatcgtcc ggtgcgggtt cgccgttggt tacctttcga ccgccagcgc gacgcccatt
      961 ccgccgccga tgcacaacga cgcgaggcct cgcttcgacc cgcgacgctt catctcgtgc
     1021 agcagcgtga ccagaatgcg gcagcccgac gcgccgatcg gatgcccgat cgcgatcgcg
     1081 ccgccattga cgttgatgcg acctgtatcc cagcccattt ccttgttgac tgcgcacgcc
     1141 tgcgccgcaa acgcctcgtt gatttccatc aggtcgagat cggccgcctt ccacccggcc
     1201 ttctccagcg ccttgcgcga cgcgggcgcc ggacccatgc ccatgatctt cgggtcgacg
     1261 ccggccgaaa cgaacgacgc gatgcgcgcc agcggcgtga gtccgagcgc cgctgcgcgc
     1321 gcttcggaca tgaccacgac ggccgcggcg ccgtcgttga tgcccgacgc attgccggcg
     1381 gttaccgttc cttccttgtc gaatgcgggt ttcagtccgg cgagcgtctc cgcattggac
     1441 ttgcgattga tgaactcgtc ggtgtcgaac gcgaccgggt cgcctttcct ctgcggaatc
     1501 atcaccgcga cgatctcttc cttgaacctg cccgcgtcct gcgcggcggc ggccttctgc
     1561 tgcgacgcca gcgcgaatgc gtcctgttgc gcgcgatcga tcgcgtactg cttcgcgacg
     1621 ttctcggccg tgatgcccat gtggtacttg ttgtacacgt cccacaggcc atcgacgatc
     1681 atgctgtcga tcatctttgc gtcgcccatg cggaacccgt cgcgcgaatt catcagcacg
     1741 tgcggcgcca ggctcatgtt ctcctggccg ccggccacca cgacctcggc gtcgccgttg
     1801 gcgatcgcct gcgcggcgag catcactgct ttcaggcccg agccgcacac cttgttgatc
     1861 gtcatcgccg gcacggccac cggcagcccc gccttgatca atgcctggcg cgccgggttc
     1921 tgcccgcatc ccgcggccag cacctggccc atgatgactt cttcgacccg ttccggtgga
     1981 agcttggcgc gcgccagcac ctccttgatg acgatcgcgc ccaggtccgc cgccgacgtc
     2041 ttggccaatg cgccgccgaa cttgcctacc gcagtcctgg ccgcggccac gatcaccgcc
     2101 ttgctcatct cgatctccta cgctttcgct ttcacaaaac gtccgggcgc cggctcgatc
     2161 accttgtact gcgcgttgcc gtacttcttc ggcgccgcca ccatgccgcc gccatactgc
     2221 gcaaaccacc tgttccagtc cgcccaccag ctgccctgat gctcggtggc gccgtggcgc
     2281 cacgcctcgc tgttggcggg caacgtcggc gacgtgctgg cccagtagct gcgcttgttc
     2341 ttcgagaccg ggttgatcga gccggcgata tggccgcttg ctccgagcac gtagcgtacg
     2401 ccgccctctc cggcaccggt cagcaatcgc gcggatgcgt acgcggcctt ccacggcacg
     2461 atgtgatcgt cgaccgcggc gaacacatag gccggcgcct tgatgctgcc gaggtcgatc
     2521 ggcttgccac agcacttgag cttgccggga atgcgcaggt tgttctcgag gtacatgttg
     2581 cgcagatacc aggcgtacat cggccccggc agattggtgt tgtcgctgtt ccagtacagg
     2641 aggtcgaagg ccatcggctg cttgccttcg agatagttgt tgacgacgta gttccatacc
     2701 aggtcgttgg cgcgcaggat cgaaaacgtg ttgccgagtt ccttgcccgg catcaccccg
     2761 cccttgccga gcgcacgctc gcgcattcgc acatgctgct cgtcgatgaa gacttcgagc
     2821 acgccggcgt cttcgaaatc cagcagcgac gtcatcaggg tcaggctcgc ggccggctgt
     2881 tcgcccttcg cggccagcac cgccagcgcg ctcgcgagga tggtgccgcc gacgcagaag
     2941 ccgagcgcgt tgatccggtt gtccttcttg cccttcaggc cgcagatatc ctgcactgcg
     3001 cggagcgccg cgatcgcgcc tttttcgagg tagtcgtccc acgtcaggtg gccgagatcc
     3061 tcgtgcacat tgcgccacga gaccaggaat gtcgtgttgc cctgctcgac cgcgaaccgc
     3121 acgaacgagt tctccggctg caggtccatg atgtagaact tgttgatcgc cggcggaagg
     3181 atcaggaacg gccgcgcatg caccttgtcg gtcagcggct tgtactggat cagctggatc
     3241 agctcgttct catagacgac cgcgccttcg ctggttgcca cattgcggcc gatctcgaac
     3301 gctttctcgt cggtctgcgt gatcttgcct ttgcggatgt cgtccagcag gttccgcatg
     3361 cccgccttca ggctctcgcc cttggtctcg atcagcgtct gctgcgcttt cggattgagg
     3421 gcgaggaaat tggccggcga catcgcatcg acccactgcg acacggcgaa cttcacgcgc
     3481 cgcttggtct tgcggtcggc atcgaccgaa tcggcgagcg cgttcatgaa ctcggagttc
     3541 agcaggtacg tgcgcgcgta gaacgaggcg agcgggttct gctgccacgc cgggtccgcg
     3601 aaccggttgt cgcggatcgg ctcggatgac ttgtcgggat gctccatgaa gtcggaccac
     3661 agccgcccca tccgctgcac gtactcctgt tgcagctgca gcactcgctc cggcgccagg
     3721 ccgatccgcg gcacgtccgc cccggtgggc gccatcgccg ccagtgacga ccaccagttg
     3781 agccaggggt tgtcgttcgc gtgcgcttcg gcgccgcgca ggctgccagc catcgcaggg
     3841 gactccggtt ccgttcgcga ccgactgggt ttacgcgcag actgcgtcgc catctgttct
     3901 tctcctgaga ttcgtgcggg gatttcgctc gtgcatgcgc gagcatcggg gctcgacgca
     3961 ttctccgcat cgccgtagca gccgtcaaac cagggcggtc agccttcgcg ccagatcacg
     4021 gcggcatgcc gcccggtgat ccggtcgcgc cgaaagctga agaagcgcgc agggtcgctg
     4081 aaggtgcaca acgtaccgcc gcccacatcc atcacaccga cctgcgccag cgcctgccgc
     4141 gccagcgccg gcagatcggc gcggaacttg ccgtccgcaa gaggcgcaaa ggcatcccgc
     4201 gcgcgggaaa gcgtgcattc catcgcttcg agaacctcga tgccgacctc gaagtgcttt
     4261 ggtccgatcc ccggcccgac ccatgcgcgg atgcgcgcgt ccggatcacc gaggcgcgcg
     4321 cgcagcgccg tgacggtcgc ctgcagcacg ccggccgcca gtccccgcca gcccgcgtgc
     4381 gcaaccgcga ccccacgtcc gctctggtcc gcgaagaaga cgggcaggca gtcggcgatc
     4441 gaaaccacgc acacgacgcc cgtctgcacg gtgaaagatg cgtcggcttc tgccggcgtc
     4501 gcgatctgtg ccgcgtcgac gacgcccgcg ccgtggacct ggcgcagcca gcgcggatcg
     4561 gccggcaccg tcgcccgcaa gacgcgccgg ttctcgttca cgaactcgag atcgtcgccg
     4621 gtcgaacgac cgaggttcag gctctcgtac gggccggtcg acacgccgcc ggcgcgcgtc
     4681 gtgacgaagg cgtgcacgcg cgccgcaacg ctccagtcgg gaacaatcca gtccggatgc
     4741 acgcgcgctg tcattcgaac aggtcgcgcg gccgatcgca gcggccaaag ccgaccgctt
     4801 gcatcagttc gcgcatgtcg gcaggtggcg gcctgaacca ttcccgcacg tcgccgcgca
     4861 gcgggtgctt gaacgccagc cggcacgcgt gcaacgcctg gcgcggaaag ctcgcccatg
     4921 cggcggcgtc ccgaccggat tgcgccagcg tccgcccgcg ccgatagagc gggtcgccga
     4981 ccagcggatg gccaatggat tcgaggtgca cacggatctg gtgcgtgcgg ccggttgcca
     5041 ggcggcacgc gagccacgac aacgatgccg ggccggcgtc aacgcactcg atgcggcgaa
     5101 agcgcgtctg cgcctcacgc gccgacggcg cgcggctcac cttgaacttg accggattgc
     5161 gcgggtcgcg cccgatggca gcgtcgatcg tgccagacgg cggcgcgctt ccgatcacga
     5221 tcgcccagta ctcgcgcagc acggtgcgcg cctgcaactg ccgcacgagg tcagtctgcg
     5281 cctcgatcgt ccgcgccacg accatcaggc cactggtgtc ggcatcgagc cggtgcacga
     5341 tgcctgcgcg cggtacgcgt gccaggcccg ggtcatgcgc cagcaggccg ttgagcagcg
     5401 tgccggacca gttgccggcg cccggatgca cgacgagacc gggcgccttg tcgagcacga
     5461 tgatcgcgtc gtcctcgtgc acgatgtcga gcgcgatcgg ctcgggggcg aatgcaagtg
     5521 cgtcgggctc cggcggcggc gcgacctcga tcacatcgcc catgagcacc gcatcgcgtg
     5581 cgcgcgtcgg cgccccgttc acccggaccg caccctgctc gatccattgc tggatacggc
     5641 tgcgcgacac ctgcggcagc agcctggcca gaaccttgtc gacgcgttcg ccggcaagtg
     5701 ccgcgctcac cgtcagtcgc tgcagcgcct cggcagcttc ggtgtcgtcg agatcgccgt
     5761 tgcgcgcgga atcgttcatc gccgcgccga tataatcgtg cgctgctgca ccttcggact
     5821 cccgcgtgtt cgacaccttg attctgctgc gcgcactgcg cgacctcttg gccaggctgc
     5881 tggccggctg cgcactcacc ctgctcgc
//
LOCUS       DBNHBY010000702         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1066363, whole genome shotgun sequence.
ACCESSION   DBNHBY010000702 DBNHBY010000000
VERSION     DBNHBY010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1066363"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..426)
                     /locus_tag="AC16HB_16665"
     CDS             complement(112..426)
                     /locus_tag="AC16HB_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608475.1"
                     /translation="MFCIAIPLFALAICSKPVPVVEDVRPVRTMVVAPAQISSIAELA
                     GEVRPRVAARVGFLVGGHIVMRAVEVGQTVKAGRVLSTIDPAGARLAALAARAQLAAR
                     SA"
     gene            complement(525..920)
                     /gene="crcB"
                     /locus_tag="AC16HB_16670"
     CDS             complement(525..920)
                     /gene="crcB"
                     /locus_tag="AC16HB_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR5608476.1"
                     /translation="MTRDGPLWAVLLAVGAGAALGACLRWALAYWFNPRVASLPLGTL
                     AANLVGGYLIGVAVAIFAAHPALSPTWRLVVVTGFLGGLTTFSTFSAESVALLHGGQW
                     GAAALHTVLHLVGSVLATFAGLATVRALG"
     gene            complement(917..1372)
                     /gene="moaE"
                     /locus_tag="AC16HB_16675"
     CDS             complement(917..1372)
                     /gene="moaE"
                     /locus_tag="AC16HB_16675"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HIR5608477.1"
                     /translation="MAVRVQREDFDAGAEVARLRANDPKVGAVACFIGVARDVNDGAQ
                     VGTLTLEHYPGMTDRALEDICAQARARWNIIDILVVHRVGDLKPTDQIVLVVVTGAHR
                     GEAFAACQFVMDYLKTEAPFWKKEATPAGARWVEARASDDEARQRWERS"
     gene            complement(1374..1631)
                     /gene="moaD"
                     /locus_tag="AC16HB_16680"
     CDS             complement(1374..1631)
                     /gene="moaD"
                     /locus_tag="AC16HB_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIR5608478.1"
                     /translation="MKVTVLYFARLREVIGRDRDEFALPADVKTLGALRAWLVARGMP
                     WSAAFDEVKRIRAAVGQAMATDDTPLADGAEVAFFPPVTGG"
     gene            complement(1628..2830)
                     /locus_tag="AC16HB_16685"
     CDS             complement(1628..2830)
                     /locus_tag="AC16HB_16685"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5608479.1"
                     /translation="MLSVEQALERLLARAKPLPGLERVSTLDAASALGRVLAADVVAH
                     IDVPPMDNSQMDGYAVRSADLVSVPATLKVAQRIAAGHVGSELAPGTAARIFTGAPVP
                     PGADAIVMQEAAVAQGDAVTINEQPKPGQWIRRRGLDVRAGATILAAGRRLGPQDLGL
                     AASVGVAELEVRRRLRVVVFFTGDELVMPGEALPPGRIYNSNRFMLRALLHAAGCDVI
                     DLGIVPDDRPATRTAFVRAAQDAELIITSGGVSVGEEDHVKPAVEAEGELDLWQIAMK
                     PGKPLAFGHVRDVPFIGLPGNPVSSFITFLLLARPFIRVRQGAADIAPHALALRADFD
                     WPKPDKRREFLRARYNAAGGLDLFTNQNSAVLTSCVWGDGVVDNPPAQPIAHGDTVRF
                     LPFTELLA"
     gene            complement(2830..>3198)
                     /gene="mobB"
                     /locus_tag="AC16HB_16690"
     CDS             complement(2830..>3198)
                     /gene="mobB"
                     /locus_tag="AC16HB_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR5608480.1"
                     /translation="FDMDRPGKDSYRYREAGAGQVLIATGLRWALLTETPRHAATLEE
                     LMALLAPCDLVIVEGFKSEGHIPRIEVRRTANSEPPIFPHDPNVVAVAADHGVDTPLP
                     LLDLNDAAKIADFIVAHLKL"
BASE COUNT          526 a         1154 c         1063 g          455 t
ORIGIN      
        1 gcactgcggc ctcgacgatc gctttccagg tgtcgacgcc gcgctcctcg tcgcgctcga
       61 tccggtcgac caggatgacc gcgttgcgga tcatcatccc caccagcgca atcacgccga
      121 gcgcgccgcc agttgcgctc gcgccgccaa ggccgcgagg cgggcgccgg ccgggtcgat
      181 cgtggacagg acccgcccgg ccttcacggt ttggccaact tcgaccgcgc gcatgacgat
      241 gtggccgccg accaggaagc cgacgcgcgc ggcgacgcgc ggccgcacct cgcccgcgag
      301 ctctgcgatc gaggaaatct gcgcgggcgc gacgaccatc gtacgcaccg ggcgtacgtc
      361 ctcgacgacc gggaccggct tcgagcaaat cgcgagcgcg aagagcggga tcgcgatgca
      421 gaacacgcga acgggaggtt gaatcggggc gccggtcttt caaatgacgc accggtcata
      481 acatagtcgc caccctcggc tgcgacaacg gagtgctcct gcagtcaccc gagtgcgcgc
      541 actgtcgcga gcccggcgaa cgtggccagt acggagccca caaggtgcaa cacggtgtgc
      601 agcgccgccg caccccattg gccgccgtgc agcagcgcga ccgactcggc ggaaaacgtg
      661 gagaacgtcg tgagtccgcc gagaaagccc gtgacgacga ccaatcgcca cgtcggcgac
      721 agcgccggat gcgcggcgaa tatcgccacc gccacgccaa tcaggtaccc gccgacgagg
      781 ttcgctgcca atgtgccgag cggcagcgac gcgactcgcg gattgaacca gtaggcgagc
      841 gcccagcgca gacatgcgcc gagtgcggct cccgcgccga cggcaagcag cacggcccac
      901 agcggaccgt cgcgcgtcaa ctccgctccc agcgttgccg cgcttcatcg tcgctggcgc
      961 gcgcttcgac ccagcgcgcg ccggccggcg tggcctcctt cttccagaac ggcgcttcgg
     1021 tcttcaggta atccatcacg aactggcagg cggcgaatgc ctcgccacga tgcgcgcccg
     1081 tgaccacgac cagcacgatc tggtcggtcg gcttcagatc gccgacacgg tgcacgacta
     1141 ggatgtcgat gatgttccag cgcgcacgcg cctgcgcgca gatgtcttcc agcgcgcggt
     1201 ccgtcatgcc gggatagtgc tcgagcgtca gcgtgcccac ctgcgccccg tcgttgacat
     1261 cgcgcgccac gccgatgaag cacgcaaccg cgccgacctt cggatcgttg gcgcgcaggc
     1321 gcgcaacctc cgccccggcg tcgaagtctt ccctctggac ccggactgcc atttcagcct
     1381 ccggtcaccg ggggaaagaa tgcgacctcg gcgccgtcgg cgagcggcgt gtcgtcggtc
     1441 gccatcgcct gccccaccgc ggcgcgaatg cgcttgacct catcgaacgc ggccgaccac
     1501 ggcatgccgc gcgcgaccag ccacgcgcgc agggcgccca gcgtctttac atccgcgggc
     1561 agcgcaaact cgtcgcgatc gcgtccgatc acttcgcgca agcgggcgaa gtacagcaca
     1621 gtcactttca tgccagcagc tccgtgaacg gcagaaagcg caccgtatcg ccgtgcgcga
     1681 tcggctgcgc cggcggattg tcgacgacgc cgtcgcccca cacgcacgag gtcaatacgg
     1741 ccgagttctg gttggtgaag aggtccagac cacccgcggc gttgtagcgg gcgcgcagga
     1801 attcgcgccg cttgtccggc ttcggccagt cgaagtcggc gcgcagcgcc agcgcgtgcg
     1861 gcgcaatgtc cgccgcgccc tgccgcacgc ggatgaacgg ccgcgcgagc agcaggaacg
     1921 tgatgaagct cgacaccggg ttgccgggca ggccgatgaa gggcacgtcg cgcacatgac
     1981 cgaatgcgag cggcttgccc ggcttcatcg cgatctgcca cagatcgagt tcgccttcgg
     2041 cctcgaccgc gggcttcacg tgatcttcct cgcccaccga cacgccgccg gaggtgatga
     2101 tcagctcggc atcctgcgcc gcacgtacga acgccgtgcg ggtggccggg cgatcgtcgg
     2161 ggacgatacc caggtcgatc acgtcgcagc ccgccgcgtg cagcagcgcg cgcagcatga
     2221 agcggttcga gttgtagatg cgcccgggtg gcagtgcttc gcccggcatc acgagctcgt
     2281 cgccggtgaa aaacaccacg acccgcagcc ggcgtctgac ttcgagctct gccaccccga
     2341 ccgatgcggc gagcccgaga tcctgcggtc cgagccggcg gccggcggcg aggatcgtcg
     2401 cgcccgcccg cacgtcgagt ccgcggcggc ggatccactg gcccggcttg ggctgttcgt
     2461 tgatcgtcac cgcatcgccc tgcgcgaccg ccgcctcctg catcacgatc gcatcggcgc
     2521 caggcgggac cggcgcgccc gtgaagatgc gcgcggcggt gccgggcgcc aactcgctgc
     2581 cgacatgccc ggcggcaatg cgctgcgcga ccttcagcgt cgccggcacg gagacgaggt
     2641 ccgcactgcg caccgcatag ccgtccatct gcgagttgtc catcggcggc acgtcgatgt
     2701 gcgcgacgac gtcggcggcg agcacgcgcc caagcgcgct cgcggcatcg agcgtggata
     2761 cgcgttccag ccccggcaac ggcttggctc gcgcgagcag ccgctccagc gcttgttcga
     2821 cggacaacat cagagcttca gatgcgcgac gatgaagtct gcaatcttag cggcgtcgtt
     2881 caggtccagc agcggcagcg gtgtatcgac gccgtggtcc gcggcaaccg cgacgacgtt
     2941 ggggtcgtgc gggaagatcg gcggctcgga gttggccgtg cgtcgcacct cgatgcgcgg
     3001 aatgtggccc tcgctcttga acccttcgac gatcaccaga tcgcaaggcg cgagcagcgc
     3061 catcagttcc tcgagcgtgg ccgcatgccg cggcgtctcg gtcagcagcg cccagcgcag
     3121 tccggtcgcg atcagcacct ggcccgcgcc ggcctcgcga tagcgatacg agtccttgcc
     3181 cggccgatcc atgtcgaa
//
LOCUS       DBNHBY010000703         6008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_51984, whole genome shotgun sequence.
ACCESSION   DBNHBY010000703 DBNHBY010000000
VERSION     DBNHBY010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_51984"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="AC16HB_16695"
     CDS             <1..1117
                     /locus_tag="AC16HB_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5608481.1"
                     /translation="GLLGRIDFFLAETEKTLAPRDVFDESGMRERITHEPLGVIANIS
                     AWNYPYFVGANVFVPALLTGNAVLYKPSEFATLTGLEIGKLLHASGVPQDVFQVIVGA
                     GAAGSSLLSQPLDGVFFTGSYATGARIAQAVGPRLVKLQLELGGKDPAYVCDDVDVAA
                     AAASTADGAMYNTGQSCCSVERIYVHAKIHDAFVDAFVKEVKGFRIGDPTGEDSYIGP
                     LTRAPQLAVLADQVADAQAKGAKLLVGGRRIEQPGNWFEPTVFANVDHTMKLMRDESF
                     GPIIGIQKVASDDEAVRLMNDTEYGLTAAVYSKDRTRAERLLARLNAGSVYWNCCDRV
                     SPRLPWSGLGHSGIGLTLSTYGIETFTRPKAWHLRSA"
     gene            complement(1121..1552)
                     /gene="trxC"
                     /locus_tag="AC16HB_16700"
     CDS             complement(1121..1552)
                     /gene="trxC"
                     /locus_tag="AC16HB_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HIR5608482.1"
                     /translation="MRVMILQCPHCHARNRVQEERFNDEPTCGSCGKPMLRGAPLALD
                     DDNFFAVIAASRKPIVVDFWAAWCGPCRIFAPVFAQAAAGRDDVLFAKVDTDASPHVA
                     SRYAIRSIPTIGLFKDAQMAERVSGALPAGQFDAWLRAAMT"
     gene            complement(1610..3661)
                     /gene="fusA"
                     /locus_tag="AC16HB_16705"
     CDS             complement(1610..3661)
                     /gene="fusA"
                     /locus_tag="AC16HB_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5608483.1"
                     /translation="MAFATENLRTVAVVGHGAAGKTTLVEQLLVKSKMIGAPGSVEKG
                     TTVSDYDPQEKTALHSLRSAVAHCDHIGTRIHVIDTPGYPDFIGQAIGALDAVETVAV
                     VVNAVNGIELVTRRMMDWAKARNLCRMIVVNKIDHEHIDLPGLLEELQQAFGQEVLPI
                     NLPAEKGNRVIDCFDHDDGTADFLSVAEVHRKLMEQVVEVDDAAMERYLEQGSVDPTT
                     LHAPLEKALRDGHIIPLCFTSARTGAGVQDLLDVMARHLPHPGEGNPPLLIKGEGAEA
                     QAFRAAPDANKHVIAHVFKVVNDPYVGKIGVFRVHQGRIGKDTPLLVGDSKKPFKVAH
                     PMLLQGKDMVEVPQLVPGDIGAVAKVEEIVFDCVLHDSHDEDFVRMKPLDFPKPMYGL
                     AVEPKRRGDEGRISEVMTKIAAEDPTLAIEHDVHQNETVLRGLSELHLRSVLERMNAL
                     YRLEVATRPPRVPYRETINAQAEGHARHKKQTGGAGQFGEVYLRVEPMGRGQGFEFVD
                     QVKGGVIPYNFIPAVEKGVREVLATGYVAGYPLQDVRVVVYDGKHHPVDSKEVAFVAA
                     GRKAMLDALPKARPIVLEPIVNIEIVVPDAVMGDITGDLSSRRGQVTGTDNLAGGMMI
                     VKGVVPLSELDGYSGRLKAMTQGQGSYSMELSHYEAVPPNVQAQLANEFKSKRKEAEE
                     D"
     gene            3797..>6008
                     /locus_tag="AC16HB_16710"
     CDS             3797..>6008
                     /locus_tag="AC16HB_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR5608484.1"
                     /translation="MAEEQRLSVPTVPPAHTRIVIIGGGIMGCSVAYHLSALGERDVV
                     LLEQGRLTSGTTWHAAGLVGQLRNHESMTRLIRYSTELYSRLEAETGLSTGWKQCGSL
                     AVARTADRMVQLKRTAAVAKAYGVTCDVIAPAEAGRLYPIMATHDLAGAVWLPGDGKA
                     NPADLTLALAKGARNRGVKICEGVRVTGATHFRTERRRGVKSLAWQAKDGTRGDIEAE
                     IVVLCGGQWSRALGRMLGVTVPLFSCEHYYIVTDRIDGVHRDLPVIRDPDGYIYFKEE
                     VGGLLMGGFEPQATPWLQSATHRGGIPENFEFQLLPDNWDAFQILLDNAMIRVPALAN
                     AQVKQFYNGPESFTADNNFIVGPAPGLDNLYVGCGFNSMGIASSGGAGKALAEWIVQG
                     EPTLDLWPVDIRRFARFNGNETWLPDRIAEVLGMHYKMPWPNRELESARPFRRSPLYA
                     HLRERGACFGSRMGWERANFFAPTPEQAKIEYGWGHQNWHPWVAAEHRACREAVAVFD
                     MTSFAKLLVQGPDAWTALQWLVANAVPRAPGATVYTGMLNERGGYESDFTITCVGADE
                     YMIVTSSGSAVRDRDVIERALRERGWRCTVTDVTSMYAMLAVMGPRARELMSRVSTAR
                     FDSASFPFGTSQEIDVGYATVRATRLTYVGELGWELYTPAEFAVGVYEQLLAAGRDLG
                     RVNAGYYAIDSLRLEKGYRAWGRELTPDVNPFEAGLSFAVRLDKDGGFRGCDALV"
BASE COUNT          960 a         2052 c         2006 g          990 t
ORIGIN      
        1 cgggctgctc ggccgcatcg acttcttcct cgccgaaacc gagaagacgc tggcgccgcg
       61 cgacgtgttc gacgagtccg gcatgcgcga gcgcatcacg cacgagccgc tcggcgtgat
      121 cgccaacatc tcggcgtgga actacccgta cttcgtcggt gcgaacgtgt tcgtgccggc
      181 tctgctgacc ggcaacgcgg ttctgtacaa gccgagcgag ttcgccaccc tgacggggtt
      241 ggagatcggc aagctgctgc acgcgagcgg cgtgccgcag gacgtgttcc aggtgatcgt
      301 cggcgcgggc gcggcgggca gttccctgct ctcgcaaccg ctcgacggcg tgttcttcac
      361 cggcagctac gcgacgggtg ccaggatcgc gcaggcggtc ggcccgcggc tggtcaagct
      421 gcagctcgaa ctcggcggca aggatccggc gtacgtgtgc gacgacgtgg acgtcgcggc
      481 cgccgctgcg tcgaccgcgg acggcgcgat gtacaacacc ggccaaagtt gctgctcggt
      541 cgaacgcatc tacgtgcacg cgaagatcca cgacgcattc gtcgatgcgt tcgtgaagga
      601 ggtgaagggc ttccgcatcg gcgacccgac gggcgaggac agctacatcg gcccgctgac
      661 gcgcgcgccg cagctggcgg tgctggccga tcaggtcgcc gacgcgcagg ccaagggtgc
      721 gaagctgcta gtcggcggca ggcgcatcga gcaacccggc aactggttcg agccgaccgt
      781 gttcgcgaac gtcgaccata cgatgaagct gatgcgcgac gagagcttcg gcccgatcat
      841 cggcatccag aaggtcgcga gcgacgacga agcggtgcgg ctgatgaacg acaccgaata
      901 cggcctgacc gcggcggtgt actcgaagga ccgcacgcgc gccgaacgcc tgctcgcgcg
      961 gctgaacgcg ggcagcgtct actggaactg ctgcgaccgc gtcagcccgc ggctgccgtg
     1021 gtcaggactt ggccactccg gcatcgggct cacgctgtcg acctatggca tcgagacatt
     1081 cacgcgaccg aaggcgtggc acctgcgatc ggcgtagggt tcacgtcatc gccgcgcgca
     1141 accatgcatc gaactgcccc gccggcagcg cgccgctgac gcgctcggcc atctgggcgt
     1201 ccttgaacaa cccgatcgtc ggaatgctgc ggattgcgta gcggctcgcc acgtgcgggc
     1261 tggcgtcggt gtcgactttc gcgaacagca catcgtcgcg gcccgcggca gcctgcgcga
     1321 acaccggcgc gaaaatccgg cacggtccgc accacgcggc ccagaagtcg accacgatcg
     1381 gcttgcggct ggcggcgatc accgcgaaga agttgtcgtc atcgagcgcc aacggcgcgc
     1441 cgcgcagcat gggtttgccg cagctgccac aggtcggctc gtcgttgaag cgttcctcct
     1501 gcacgcgatt cctggcgtgg cagtgggggc attggaggat catgacccgc atgtggatgc
     1561 accacgcgcc gattcaagcg cgcgtgtgat cctcgtcacg tgaacgcggt cagtcctctt
     1621 cggcttcctt gcgcttgctc ttgaactcgt tcgccagttg cgcctgcacg ttgggcggca
     1681 ccgcctcgta gtgcgacagt tccatcgagt agctgccctg cccctgcgtc atggccttca
     1741 gtcgccccga gtagccgtcc agctcggaca gcggaaccac gcccttgacg atcatcatgc
     1801 cgccggccag gttgtcggtg ccggtgacct gcccgcgccg cgacgacagg tcgccggtga
     1861 tgtcgcccat aaccgcatcg ggcacgacga tctcgatatt gacgatgggc tcgagcacga
     1921 tcggccgcgc cttcggcagc gcgtcgagca tggccttgcg ccccgcggcc acgaacgcga
     1981 cctccttgct gtcgaccgga tggtgcttgc cgtcgtagac gaccacgcgg acatcctgga
     2041 gcgggtagcc cgcgacatac ccggtcgcaa gcacctcgcg cacgcccttt tcgaccgccg
     2101 gaatgaagtt gtagggaatc acgccgccct tgacctgatc cacgaactcg aagccctgcc
     2161 cgcgtcccat cggctccacg cgcagataca cctcgccgaa ctggccggcg ccgccggtct
     2221 gcttcttgtg gcgcgcgtga ccttccgcct gcgcgttgat cgtctcgcgg tacggcacgc
     2281 gcggcgggcg cgtcgcgact tcgagcctgt acagcgcgtt catccgttcc agcacggagc
     2341 gcaagtgcag ttcggacaac ccgcgcagca ccgtctcgtt ctggtgcacg tcatgctcga
     2401 tcgcgagcgt cgggtcctcg gcggcgatct tggtcatcac ctccgagatg cggccctcgt
     2461 cgccgcgccg cttgggctcg accgcgaggc cgtacatcgg cttcggaaaa tcgagcggct
     2521 tcatgcgcac gaaatcctcg tcgtgcgagt cgtgcagcac gcagtcgaac acgatctcct
     2581 cgaccttcgc caccgcgccg atgtcgccgg gcacgagctg cggcacctcg accatgtcct
     2641 tgccctgcag aagcatcggg tgcgcgacct tgaacggctt cttgctgtcg ccgaccaaga
     2701 gcggcgtgtc cttgccgatc cggccctggt gcacgcggaa cacgccgatc ttgccgacgt
     2761 acggatcgtt gacgaccttg aacacgtgcg cgatcacgtg cttgttcgca tcgggcgcgg
     2821 cgcgaaacgc ctgtgcctcc gcgccctcgc ccttgatcag cagcggcggg ttgccctcgc
     2881 cgggatgtgg caggtggcgc gccatcacgt cgagcaggtc ctgcacgccg gcgccggtgc
     2941 gcgccgaagt gaagcacagc gggatgatgt gcccgtcacg cagtgccttt tcgagcggcg
     3001 cgtgcagcgt ggtcggatcg acgctgccct gctccagata tctttccatc gccgcgtcgt
     3061 cgacctcgac cacctgctcc atcagcttgc ggtgcacttc ggcaaccgac aggaaatcgg
     3121 ccgtgccgtc gtcgtggtcg aagcagtcga tcacgcggtt gcccttttcc gccggcaggt
     3181 tgatcggcag cacttcctgg ccgaacgcct gctgcagttc ctccagcagc cccggcaggt
     3241 cgatgtgctc gtggtcgatc ttgttgacga cgatcatccg gcacaggttg cgcgccttcg
     3301 cccagtccat catccggcgc gtgacgagct cgatgccgtt gacggcattg acgaccaccg
     3361 cgaccgtttc gaccgcgtcg agcgcgccga tcgcctggcc gatgaaatcc ggatatcccg
     3421 gagtgtcgat gacgtgtatc cgcgtaccga tgtgatcgca atgcgcgacc gccgagcgca
     3481 gggaatgcag cgcggtcttc tcctgcgggt cgtagtcgga cacggtggtg cccttctcca
     3541 cgctgcccgg cgcgccgatc atcttcgact tgaccagcaa ctgctcgacc agcgtcgtct
     3601 tgcccgcggc accgtggccg acgacggcaa cggtgcgcag attctccgtg gcgaaagcca
     3661 ttgttctcct cgctcctgag tggtcggcgc agactataac gcccgccgcc gcgcgcggca
     3721 ggtgaccggc agcaggtggt gcgggcacaa tgtgaggcag aacaacaaac cgtccgcgct
     3781 tcccgactcc actgcgatgg ccgaagagca acgcctgtcc gtgccaacgg tgccgcccgc
     3841 gcacactcgc atcgtgatca tcggcggcgg catcatgggc tgttcggtcg catatcacct
     3901 gagcgcgctc ggcgagcgcg acgtggtgct gctcgaacag gggcggctca cttccggcac
     3961 cacgtggcac gcggccgggc tcgtcgggca gttgcgcaat cacgagagca tgacgcggct
     4021 gatccgctac tcgaccgagc tgtactccag gctcgaagcg gaaacggggc tctccaccgg
     4081 ctggaagcag tgcggatcgc tcgcggtcgc gcgcacggcg gaccgcatgg tgcaactgaa
     4141 gcgcaccgcg gcggtggcca aggcgtacgg cgtcacctgc gacgtgatcg cgcccgccga
     4201 ggccggcagg ctctatccaa tcatggccac acacgatctg gccggcgcgg tgtggttgcc
     4261 cggcgacggc aaggccaatc cagcggacct gacgctcgcg ctcgcaaaag gggcgcgcaa
     4321 ccgtggcgtc aagatctgcg aaggcgtgcg cgtgaccggc gcgacgcact tccgcaccga
     4381 gcgccgccgc ggcgtgaagt cgctcgcgtg gcaggccaag gacggcactc ggggcgacat
     4441 cgaggccgag atcgtcgtgc tgtgcggcgg ccagtggtcg cgcgcgctcg gccgcatgct
     4501 cggcgtcacg gtgccgctct tttcgtgcga gcactactac atcgtcaccg acaggatcga
     4561 cggcgtgcac cgcgatctgc cggtgatacg cgatccggac ggctacatct acttcaagga
     4621 ggaagtgggc gggctcctga tgggcggctt cgagccgcag gcgacgccgt ggctgcagag
     4681 cgcgacgcat cgcggcggca ttccggagaa cttcgagttc cagctgctgc ccgacaactg
     4741 ggacgcgttc cagatcctgc tcgacaacgc gatgatccgc gtgccggcac tcgcgaacgc
     4801 gcaggtcaag cagttctaca acggtcccga gtcgttcacc gcggacaaca acttcatcgt
     4861 cggtccagcg ccgggcctcg acaatctcta tgtcggctgc ggcttcaact cgatgggcat
     4921 cgcctccagc ggcggcgccg gcaaggcact ggcggagtgg atcgtgcagg gcgagccgac
     4981 gctggacctg tggccggtcg acatccgccg cttcgcccgc ttcaacggca acgagacatg
     5041 gctgcccgac cgcatcgccg aagtgctcgg catgcactac aagatgccgt ggccgaaccg
     5101 cgagctcgaa agcgcgcgcc cgttccgccg ctcgccgctg tacgcacatc ttcgcgaacg
     5161 cggcgcctgt ttcggctcca ggatgggctg ggagcgcgcc aacttctttg cgccgacgcc
     5221 ggagcaggcg aagatcgagt acggctgggg gcaccagaac tggcacccct gggtcgcagc
     5281 cgagcaccgc gcctgtcgcg aggccgtggc cgtgttcgac atgacgtcgt tcgccaagct
     5341 gctcgtgcag ggaccggacg cgtggaccgc gctgcagtgg ctggtcgcca acgcggtgcc
     5401 gcgggcgccc ggcgcgaccg tctacaccgg catgctgaac gaacgcggcg gctacgagag
     5461 cgacttcacg atcacctgcg tcggcgccga cgagtacatg atcgtcacca gttccgggtc
     5521 ggccgtgcgc gaccgcgacg tgatcgaacg cgcgctgcgc gaacgcggct ggcgctgcac
     5581 ggtgacggac gtgacgtcga tgtacgcgat gctcgcggtg atgggaccgc gcgcgcgcga
     5641 actgatgagc cgcgtgtcga ccgcgcgctt cgacagcgcg tcgttcccgt tcggcacctc
     5701 gcaggagatc gacgtcggct acgcgaccgt gcgcgcgacg cggctcacct atgtcggcga
     5761 actgggctgg gagctgtaca cgccggccga gttcgcggtc ggcgtgtacg aacaattgct
     5821 cgcggcgggg cgcgacctgg ggcgcgtgaa cgccggctac tacgcgatcg actcgctgcg
     5881 cctggagaag ggctaccgcg cgtggggacg cgaactcaca cccgacgtca atccgttcga
     5941 ggccggcctg tcgttcgcgg tcaggctcga caaggatggc ggcttccgcg gctgcgatgc
     6001 gctggtcc
//
LOCUS       DBNHBY010000704         7542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_323179, whole genome shotgun sequence.
ACCESSION   DBNHBY010000704 DBNHBY010000000
VERSION     DBNHBY010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_323179"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16HB_16715"
     CDS             complement(<1..712)
                     /locus_tag="AC16HB_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5608485.1"
                     /translation="MSVREGSLEAPTRHPLDWKNPDFYDEEALNKELHRVFDICHGCR
                     RCVSLCGAFPTLFDLVDNSPTTEMDGVPKEKYRDVVDQCYLCDMCFMTKCPYVPPHPW
                     NLDFPHLMLRAKAVHFRKHGASAVDKFMTSTDKLGMFAGIPIVTQAVNAVNRTAPARA
                     AQESLFGLERNAWRPEFGARKFRSVARSNMGAAVKDGAKTPGKVAIYSTCYVNYNEPG
                     IGHDLIRILEHNAIPYVLA"
     gene            complement(835..1257)
                     /locus_tag="AC16HB_16720"
     CDS             complement(835..1257)
                     /locus_tag="AC16HB_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR5608486.1"
                     /translation="MAQLKGSKTEGNLKAAFAGESQANRRYLYFAAKADVEGYNDVAA
                     VFRSTAEGETGHAHGHLEYLEQVGDPATGLPIGPTKSNLKAAIAGETHEYTDMYPGMA
                     KAARDEGFDEIADWFETLAKAERSHANKFQRTLDSIVD"
     gene            complement(1406..3211)
                     /locus_tag="AC16HB_16725"
     CDS             complement(1406..3211)
                     /locus_tag="AC16HB_16725"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR5608487.1"
                     /translation="MSYVAPVKDMLFTMRELAGLAQIQKLPGFEEATDDTVAAVLEEN
                     ARFMAEVIAPLNLTGDTRPSTWNDGKVTTTPGFKEAFRQFAEAGWQGLQHPPQYGGQG
                     LPKLVGTPCTEMLNSANLSFALCPLLTDGAIEALMTAGTHAQQKLYVPRLVDGTWTGT
                     MNLTEPQAGSDLAMVRTKAVPQGDGSYRLSGTKIFITYGEHDLADNIVHLVLARTPDA
                     PEGVKGISLFIVPKFLVKADGSIGKRNDVWCASIEHKLGIKASPTAVLLFGDNKGEVG
                     PGAIGYVVGEENRGLEYMFIMMNAARFAVGLQGVSVAERAYQMAAAYAKERVQSRAVE
                     GSSAAVTIVHHPDVRRMLMYMRANTEAARAIAYVAAAWCDIAHHHTDAAMRKQARAVY
                     EYLVPIVKGWSTEMSIDVASTGIQVHGGMGYIEETGAAQFYRDARILTIYEGTTAIQA
                     NDFIGRKTVRDGGAVAKGLIAQMRATQDDLARAASPDLVAIGRRLKSAVEDYAAVVDH
                     VVANTKGDVRGVFAGSVPYLKLAGVTHGGWQMARAALAAERGLAAGADDADFLRAKIS
                     TARFHAEHILSTSGGLRAAAIEGAAPVLALPVEAF"
     gene            complement(3233..4162)
                     /locus_tag="AC16HB_16730"
     CDS             complement(3233..4162)
                     /locus_tag="AC16HB_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5608488.1"
                     /translation="MTALVLAEHDQVHLRGATLNTVTAAAKAAGDVHVLVVGHNCAGA
                     AAHAAKLAGVVKVLVADAPHFADGLAENVAAQVLAVAKGYSHVFAPATAYGKNILPRV
                     AAKLDAAMVSDIISVQSSDTFTRPIYAGNAIATVQVADPVKVITARPTNFDAVAEGGG
                     AAIESVTPVADTGLSKFASRDVAKSDRPELQGAKVVVSGGRGMGSAENFKLLDPLADR
                     LGAALGASRAAVDAGYAPNDWQVGQTGKVVAPQLYIAVGISGAIQHLAGMKDSKVIVA
                     INKDPEAPIFSVADYGLVADLFEAVPALVKELG"
     gene            complement(4176..4925)
                     /locus_tag="AC16HB_16735"
     CDS             complement(4176..4925)
                     /locus_tag="AC16HB_16735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5608489.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDQTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEAGVATEIVAISCGVTQCQETLRTAMAIGADRAILVETDAELQPLAVAKILR
                     ALVEKEKPMLILCGKQAIDDDANQTGQMLAALADLPQATFVSKLKIADGAATATREVD
                     GGLETISLRLPAVVTVDLRLNEPRYVTLPNIMKAKKKPLEMVKPADLGVDPAPRIKTL
                     KVTDPPARKAGVKVPDVAALVAKLKNEAKCI"
     gene            complement(5017..5451)
                     /locus_tag="AC16HB_16740"
     CDS             complement(5017..5451)
                     /locus_tag="AC16HB_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR5608490.1"
                     /translation="MTGLQFSEEAGFLGLFVSSFTSATLLPGGSEVVLLAVVHRFPEA
                     FWRAILIATIGNTAGGMTSYLLGRLLPNRVEHRAIVQLHRYGYWALLLSWLPVIGDAL
                     CVGAGWMRFNPWLAALLLALGKCGRYIMLAGGWTWFAARSLP"
     gene            5563..>7542
                     /locus_tag="AC16HB_16745"
     CDS             5563..>7542
                     /locus_tag="AC16HB_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5608491.1"
                     /translation="MDEIAVAQFLAALERLPDLGDPAADELAQVERLLAAAPTPELRS
                     ASHARALRYIAAVRAADAGWLSAQNLLASFPLASAEGIALLRLAEALLRAPDSETQTW
                     LIAEKLAAFRAAPKGDGESLMQRVLSAALKIAGHTASDAELQAGADASAVRAWLARTT
                     LRPLVIEGIRRFGEQFVFASGLSAATARAARRPDARETYSFDMLGEGVRTGADARRNF
                     DAYAAAVAALKDTRDARPWFERDGISVKLSAIHPRLETLQFDRVERELYPRLVELAAL
                     ARDADINFAVDAEESERLELQIALVERLMREPQLRDWPGLGLALQAYLPRAGKAIEHL
                     LACAREHVRPLAVRLVKGAYWDAEIKHAQEQGLAGFPVFTHKWASDAAYAVFAERLLA
                     AGDLVYSQFATHNALTAATIVELGRRHPRSSYEFQRLHGMGAPLYAALLADQPHARVR
                     VYAPVGEFRDLLAYLVRRLLENGANSSFVHQISDKAVPAENLVEDPFVLAQRATAEGL
                     RSSRLRTGAQLFDERPNSHGIDLQHAPTIVALARQVARLAAADATPLAHGAAPPRARV
                     TVRNPARLTEPIGTIVEATSDEVPAAVAAAVGAQRGWDATPVARRAELLRRCADAFET
                     AHDDLVARLVKEAGKTLLDAHLEVREAVDFLRYSSA"
BASE COUNT         1224 a         2609 c         2416 g         1293 t
ORIGIN      
        1 ccgccagcac gtacgggatc gcgttgtgct cgaggatgcg gatcaggtca tgcccgatgc
       61 ccggctcgtt gtagttcaca tagcacgtcg agtagatcgc gaccttgccc ggcgtcttgg
      121 cgccatcctt cacggcggcg cccatgttgg agcgcgcaac cgaacggaat ttccgcgctc
      181 cgaactcggg ccgccacgcg ttgcgctcga gcccgaacag actttcctgc gccgcccgcg
      241 ccggcgcggt gcggtttacc gcatttactg cctgcgtgac gatcgggata ccggcaaaca
      301 tgccgagctt gtcggtcgag gtcatgaatt tgtcgaccgc gctggccccg tgcttgcgga
      361 agtgcaccgc cttggcacgc agcatcaggt gcggaaagtc gagattccac ggatgcggcg
      421 gcacgtacgg gcatttcgtc atgaagcaca tgtcgcacag gtaacactgg tcgacgacgt
      481 cgcggtactt ctccttcggc acaccatcca tttccgtggt cggactgttg tcgaccaggt
      541 cgaacagcgt ggggaacgcg ccgcacaggc tgacgcatcg tcgacacccg tggcagatgt
      601 cgaagacgcg atgcagttcc ttgttcaacg cctcttcgtc gtagaaatcg gggttcttcc
      661 agtcgagcgg atggcgggtc ggcgcctcca gcgacccttc gcgaaccgac atcggttttc
      721 ctttatcgag attcagctca catcagcaca gcgggccgct tcatcgcaga tgagcggccc
      781 gcccggtcac aacagtaagt ccggcgtgcg gcacgcgcgc cggaaccggt gacttcaatc
      841 gacgatcgaa tccagggtcc tctggaactt gttcgcatgc gagcgctccg ccttggccag
      901 cgtctcgaac caatccgcga tctcgtcgaa gccttcgtcg cgcgccgcct tggccatgcc
      961 cggatacata tcggtgtact cgtgcgtctc gcccgcgatc gcggctttca ggttcgactt
     1021 cgtgggcccg atcggcagcc cggtcgccgg gtcgccgacc tgctccaggt attcgaggtg
     1081 accatgcgcg tgtccagtct caccttcggc ggtcgagcgg aacaccgctg caacatcgtt
     1141 gtagccttcg acgtcggcct tggcggcgaa atacagatag cggcgattcg cctgcgattc
     1201 gccggcaaat gcagccttca ggttgccttc ggtcttcgag cctttcagtt gtgccatggg
     1261 gagtctccag actgggtcat agaagaaagc cgggcagcca cctcacgccc ggatgccaaa
     1321 actgtaagcg caatggccgc gacggaccaa tgacttctct caattcgcgc gataggtcgc
     1381 ccctattgcg cgatgccgac ccgagtcaga aagcttcgac cggaagcgcc agcaccggtg
     1441 cggcaccctc gatcgctgcg gcgcgcaggc cacccgaggt ggacaggatg tgctcggcat
     1501 ggaaacgcgc ggtcgagatc ttggcacgca ggaagtccgc atcgtcggca cccgctgcca
     1561 gcccgcgctc ggccgccagc gccgcacgcg ccatctgcca accgccgtga gtcacgccgg
     1621 cgagcttcag atacggcacc gatccggcga acacgccgcg gacgtcgccc ttcgtgttgg
     1681 ccacaacgtg gtcgaccacg gccgcgtagt cctcgacggc cgacttcagg cgccggccga
     1741 tcgcaacgag gtccggcgac gcagcgcgcg ccaagtcgtc ctgggtcgcc cgcatctgcg
     1801 cgatcagtcc cttcgccacc gcacccccgt cgcgcacggt cttgcggccg atgaagtcat
     1861 tggcctggat cgcggtcgtg ccttcgtaga tggtcaggat gcgcgcgtcg cgatagaact
     1921 gcgcggcgcc ggtctcttcg atgtagccca tgccgccgtg cacctggatg cccgtggaag
     1981 cgacgtcgat cgacatttcc gtcgaccagc ccttgacgat gggcaccagg tattcgtaaa
     2041 cggcgcgcgc ctgtttgcgc atcgccgcgt cggtgtgatg gtgcgcgata tcgcaccatg
     2101 ccgccgccac gtacgcgatc gcgcgcgccg cctcggtgtt ggcgcgcatg tacatcagca
     2161 tccgccgcac gtccggatgg tgaacgatcg tgactgccgc gctcgatccc tcgaccgcgc
     2221 ggctctgcac acgctccttc gcgtacgccg ccgccatctg gtacgcgcgc tcggcaaccg
     2281 acacgccctg caggcccacc gcaaagcgcg ccgcattcat catgatgaac atgtattcga
     2341 ggccgcggtt ttcctcgccg acgacgtagc cgatcgcgcc cggcccgacc tcgcccttgt
     2401 tgtcgccgaa cagcagcacc gcggtgggac tcgccttgat gccgagcttg tgctcgatcg
     2461 acgcgcacca gacgtcattg cgcttgccaa tcgagccgtc cgctttgacg aggaacttgg
     2521 ggacgatgaa cagcgagatc cccttcacgc cctccggggc gtcgggcgtg cgcgcgagca
     2581 cgaggtggac gatgttgtcc gccaggtcgt gctcaccgta cgtgatgaag atcttggtgc
     2641 cggacagtcg atagctgccg tcgccctgcg gtaccgcctt ggtccgcacc atcgcaagat
     2701 cggagccggc ctgcggctcg gtcaggttca tggtccccgt ccaggtgccg tcgacgaggc
     2761 gcggtacgta cagcttctgc tgcgcgtgcg tgcctgcggt catcagcgcc tcgatcgcac
     2821 catcggtcag cagcggacac aacgcgaacg aaaggttggc cgagttcagc atctcggtgc
     2881 agggtgtacc gaccagtttc ggcagtccct gcccgccgta ctgaggcgga tgctgcaatc
     2941 cctgccagcc ggcctcggca aactggcgga acgcctcctt gaatcccggt gtcgtcgtga
     3001 ccttgccgtc attccaggtc gatggcctgg tgtcgcccgt gaggttcagc ggcgcgatca
     3061 cctcagccat gaagcgcgcg ttttcctcca gcacggcggc cacggtgtcg tcggtcgcct
     3121 cctcgaagcc cggcagcttc tggatctgcg cgagcccagc cagctcgcgc atcgtgaaca
     3181 gcatgtcctt gaccggcgcg acgtagctca tgaccgactc cgtgtcagta gatcagccca
     3241 gttccttcac cagcgcgggt accgcttcga agagatcggc gaccagcccg tagtccgcga
     3301 ccgagaagat cggcgcctcc gggtccttgt tgatcgccac gatcaccttg ctgtccttca
     3361 tccctgcgag gtgctggatc gcgcccgaaa tgcccaccgc gatgtaaagc tgtggcgcca
     3421 ccaccttgcc ggtctgcccc acctgccagt cgttcggcgc gtatccggca tcgaccgccg
     3481 cgcgcgaggc gccgagcgcc gcacccagcc tgtcggcgag cggatcgagc aacttgaaat
     3541 tctcggccga gcccatgccg cggccgcccg agacgacgac tttggccccc tgcaattccg
     3601 gccggtcgct cttggcgacg tcgcgcgaag caaacttcga caggcccgtg tccgccaccg
     3661 gcgtgacgct ctcgattgcg gcaccgccgc cttcggctac cgcgtcgaaa ttcgttggcc
     3721 gcgccgtgat caccttgacc ggatcggcga cctgcacggt cgcgattgcg ttgcccgcgt
     3781 agatcggacg cgtgaaagtg tcggacgact ggacgctgat gatgtccgac accatcgcgg
     3841 catcgagctt cgccgcgacg cgcggcagga tgttcttgcc gtacgcagtc gccggcgcga
     3901 agacatgcga gtatcccttc gcgactgcga ggacctgcgc cgcgacgttt tcggccagcc
     3961 cgtcggcaaa gtgcggcgca tccgcaacca gcaccttgac cacgcccgcg agcttggccg
     4021 cgtgtgctgc cgcgcccgcg cagttgtgtc ccacgacgag cacgtgcacg tcaccggccg
     4081 ctttcgcggc cgcggtgacg gtgttcagcg tggccccgcg cagatggacc tgatcgtgtt
     4141 ctgccaaaac aagagctgtc atctcgtcct ctgcgtcaga tgcacttcgc ttcgttcttc
     4201 agcttcgcca ccagtgcggc gacatcgggc accttgacgc cggccttgcg cgcgggcgga
     4261 tcggtcacct tcagcgtctt gatccgcggt gcaggatcga caccgagatc ggcgggcttc
     4321 accatctcca gcggcttctt cttcgccttc atgatgttcg gcagcgtcac gtagcgcggt
     4381 tcgttcagcc gcaagtcgac ggtgaccacc gcgggcagcc tgagcgagat cgtctccagc
     4441 ccgccatcga cttcccgcgt cgcagtcgcc gcgccgtcgg caatcttcag cttggacacg
     4501 aacgtcgctt gcggcagatc ggccagcgcg gcgagcatct ggccggtctg gttggcgtcg
     4561 tcgtcgatcg cctgcttgcc gcacaggatc agcatcggct tctccttctc gacgagcgcc
     4621 ctgagaatct tcgccaccgc gagcggctgc aattcggcgt cggtctcgac caggatcgcg
     4681 cgatcggcgc caatcgccat cgcggtgcgc aacgtctcct ggcattgggt cacgccgcat
     4741 gaaatggcga cgatctcggt ggcgacgccg gcctccttca gccgcaccgc ctcctcgacg
     4801 gcgatctcgt cgaacgggtt catcgacatc ttcacgttcg cgatatcgac tccggtctgg
     4861 tcggacttga cccggacctt gacgttgtag tcgacgacac gcttgaccgg tacgagcacc
     4921 ttcatggctt catctttttc gagggaatga aattgcgcgg gaacagacgg gggattatac
     4981 gaatgcgccg cgcacaaacc ggagcgcgaa cacgggtcag ggcaaggaac gcgccgcgaa
     5041 ccaggtccag ccgcccgcga gcatgatgta ccggccgcac ttgccgagcg ccagcagcag
     5101 cgccgccagc catggattga aacgcatcca gcccgcgccc acgcacagcg cgtcaccgat
     5161 caccggcaac cacgacagca gcagcgccca gtagccatag cggtgcaact ggacgattgc
     5221 cctgtgctcg acgcgattcg ggagcagccg cccgagcaga taggacgtca tgccccccgc
     5281 cgtgttgccg atcgtggcga tcaggatcgc gcgccagaaa gcctctggga accggtggac
     5341 caccgcgagc agaacgacct cggagccgcc gggcagcagc gtggccgagg tgaagctgga
     5401 gacgaaaagc ccgaggaagc cggcctcctc cgagaattgc agacctgtca tcggcccgac
     5461 ggcggtgcga cagaaacggg aaatccattg tagtcagcac cgctgatgcg gcaccggcgc
     5521 ggtgatagat tgccgcggca ttcaggcgcc cggagacaag cgatggacga aatcgcggta
     5581 gctcaattcc tcgctgcgct ggaacgactc cctgacctcg gcgatcccgc cgcggacgag
     5641 cttgcgcagg tcgaacggct gctcgcggct gcgcccactc cggaattgcg cagcgcgtcc
     5701 cacgcgcgcg cgctgcgata catcgctgcc gtgcgcgcgg ccgacgccgg atggctgtcg
     5761 gcgcagaacc tgctcgcatc atttccgctc gccagtgccg agggcatcgc gctgctgcgg
     5821 ctcgccgagg cgctgctgcg tgcgcccgat tcggaaacgc agacatggct gatcgccgag
     5881 aagctcgccg cgttccgcgc ggcgccgaag ggcgacggcg aaagcctgat gcagcgcgtg
     5941 ctcagcgcgg cgctgaagat cgccggccac accgcgagcg acgccgaact gcaggccggc
     6001 gccgacgcca gcgcggtgcg cgcatggctg gcgcgcacga cgctgcgccc gctggtgatc
     6061 gaaggcatcc gacgcttcgg cgagcagttc gtgttcgcct cgggcttgtc tgcggcgacc
     6121 gcgcgcgccg cacgccgccc tgatgcgcgc gagacgtact cgttcgatat gctcggcgag
     6181 ggtgtgcgca caggggccga cgcgcgtcgc aatttcgacg cttatgcggc cgcggtcgcg
     6241 gcgctcaagg acacacgcga cgcgcgcccc tggttcgagc gcgacggcat ctccgtcaag
     6301 ttgtccgcaa tccatccccg gctcgagacc ctgcaattcg atcgcgtcga gcgcgagctg
     6361 tacccgcgcc tggtcgagct cgccgcgctc gcgcgcgacg ccgacatcaa cttcgcggtc
     6421 gatgcggagg agtccgagcg actggagttg cagatcgcat tggtggagcg gctgatgcgc
     6481 gagccgcagc tcagggactg gcccggactc gggctggcgc tgcaggcata cctgccgcgc
     6541 gcgggcaagg ccatcgagca tctgctcgcg tgcgcgcgcg aacacgtccg gccgctcgcg
     6601 gtgcgactgg tcaagggcgc gtactgggac gccgagatca agcacgcgca ggagcaaggg
     6661 ctcgcgggct ttccggtgtt cacgcacaag tgggcgagcg atgccgcgta cgccgtcttc
     6721 gccgaacggc tgctcgcggc cggcgacctc gtgtactcgc agttcgccac ccacaacgcg
     6781 ctgaccgccg ccaccatcgt cgaactcggc cggcgccatc cgcggtcgag ttacgaattc
     6841 cagcggttgc acggcatggg cgcgccgctg tacgccgcgc tgctggctga tcagccgcat
     6901 gcgcgcgtgc gcgtgtacgc gccggtcggc gagttccgcg acctgctcgc ctacctggtg
     6961 cggcgcctgc tcgagaacgg cgccaactcg tcgttcgtcc accagatttc ggacaaggca
     7021 gtgccggcgg aaaacctggt cgaggatccg tttgtgctcg cccagcgggc gaccgccgag
     7081 ggtctgcggt cgtcgcggct gcgcacgggc gcgcaattgt tcgacgagcg gccgaattcg
     7141 cacggtatcg atctgcagca cgcaccgacg atcgtggcgc tggcccgtca ggtcgcacgc
     7201 ctggccgcgg ccgatgcgac accgctggcc catggcgccg cgccgccacg ggcgcgggtc
     7261 acggtcagga acccggcgcg cctcaccgaa ccgatcggca cgatcgtcga ggcgacatcc
     7321 gatgaagttc cagcggcggt cgcggccgcc gtcggagcgc agcggggatg ggatgcaacg
     7381 cccgtcgcgc gccgcgccga actgctgcgt cgttgcgcgg atgcattcga gaccgcgcac
     7441 gacgatctcg tcgcgcgcct cgtcaaggag gctggcaaga cgctgctcga cgcgcacctg
     7501 gaagtgcgcg aggccgtcga tttcctgcgc tactcctccg cg
//
LOCUS       DBNHBY010000705         2566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_729163, whole genome shotgun sequence.
ACCESSION   DBNHBY010000705 DBNHBY010000000
VERSION     DBNHBY010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_729163"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..>2566)
                     /locus_tag="AC16HB_16750"
     CDS             complement(194..>2566)
                     /locus_tag="AC16HB_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608492.1"
                     /translation="RIRLGLERDAKQLLGADLVLSSDRPIADAERARARGAGLQTAET
                     IVFPSMAVSERDANDTALAAVKAVSTGYPLRGALRVTTQPDAPDAVTRDIPAPGTVWV
                     DAPLLQALRLAVGDRLRLGERSFRIDRVITIEPDRGAQFINFAPRVLLNIADLPSTQL
                     IQEGSRVTYRLLVAGNEAVVRAYAAWVGENLVRGQRIESLEGGRPEMQRTIERAERFL
                     ALVALLAAMIAAVAVAAAARRFSLRHLDSCAMMRCLGLAQRDIFALFTLEFVITGAAA
                     CAVGMAVGYGAHFLLLEVLRTLIQTQLPLPSFAPALQGGAVGLVLLLGFALPPLAQLR
                     HVPPLRVLRKDLGPPTGRAAFGYLLGFAGFFALLLWSSNDLKIGSLTAGGFALGIVSF
                     ALVAWLALKALAPLRAMTGRLGISWRFAVAAVQRRPVATIVQLVALAVGLMALLLLTV
                     TRTDLVDAWRKAAPPDAPNRFVINIQPDQTTAVERWLAEAGIRDVTLYPMIRGRLIEK
                     NGAVIGPENFANERAQRLVDREFNLSYTDEAPSHNAIVAGHWFRPGAAELSMEEGIAK
                     TLGIALGDELVFDIAGARVTAKVTSLRKVNWDSMRANFFVLMPSALLSDQPRSFMTAF
                     HLPPAKAAVGAELVREFPNLIVVDTSALLRQVQGVLDQVIAAVEFLFVFTLIAGVLVL
                     YAALASSRDERVREAGLLRALGASRSQLAQAQIAEMICLGGLAGLLAAAGAAAIGWSL
                     AKYAFEFEYAVTPWVFMLGIAGGATCALVGGWFGLRSVLKTPPLTTLREA"
BASE COUNT          415 a          928 c          881 g          342 t
ORIGIN      
        1 cagttccatc aggtcgtaga accgatcgac cagcgcgcgc acgccggcat cgccgccgag
       61 cagcgcgtag ggcgtttctt cagcgcgcgt tgcggtttcg gtgggattcg gaacgctcat
      121 ctggccctct tcgcgcctgc ggcgctgtcc ccccaagggg gagcgatcct tcttggggcg
      181 gcccggcgat gaatcatgct tctcgcaacg tcgtcaaggg cggcgtcttc agcacgctgc
      241 gcaggccgaa ccagccgccg acgagcgcgc aggtcgcgcc gccggcgatg ccgagcatga
      301 acacccacgg cgtgacggcg tattcgaact cgaacgcgta cttcgccagc gaccagccga
      361 tcgccgccgc gccggccgcc gccagcaacc cggcgagccc gccgaggcag atcatctcgg
      421 cgatctgcgc ctgagcaagc tggctgcgcg acgcgccaag cgcgcgcagc aggcccgcct
      481 cgcgcacgcg ctcgtcgcgg ctgctcgcga gcgccgcgta cagcaccagc acgccggcga
      541 tcagcgtgaa cacgaacagg aactcgaccg ccgcgatgac ctggtcgagc acgccctgca
      601 cctggcgcag cagcgcgctg gtgtcgacca cgattagatt cgggaactcg cgcaccagtt
      661 cggccccgac cgcggccttg gccggcggca ggtgaaacgc cgtcatgaag ctcctgggct
      721 gatcgctcag cagcgcactc ggcatcagca cgaagaagtt ggcgcgcatc gaatcccagt
      781 tgaccttgcg cagcgacgtc accttggccg tcacgcgggc gccggcgatg tcgaacacca
      841 gctcgtcgcc gagcgcgatg ccgagcgtct tcgcgatgcc ctcttccatc gacagctcgg
      901 ccgcgccggg ccggaaccag tgcccggcaa cgatcgcgtt gtgagaaggc gcctcgtccg
      961 tgtacgacag gttgaactcg cggtcgacca gccgctgcgc gcgttcattg gcaaagttct
     1021 ccggcccgat cactgcaccg ttcttctcga tcaggcggcc gcggatcatc gggtacagcg
     1081 tcacgtcgcg gatacccgcc tccgcgagcc agcgctccac ggccgtcgtc tggtccggct
     1141 ggatgttgat gacgaagcgg ttcggcgcat cgggcggcgc ggccttgcgc cacgcgtcga
     1201 cgagatcggt gcgcgtgacc gtcagcagca gcagcgccat cagcccgacc gcgagcgcga
     1261 ccagttgaac gatcgtcgcg accggacggc gctgcacggc cgcgaccgcg aagcgccacg
     1321 agatgccgag ccggccggtc atcgcacgca gtggcgccag cgccttcagc gccagccacg
     1381 cgacgagcgc gaacgacacg atgccgagcg cgaatccgcc cgcggtcagg ctgccgatct
     1441 tcaggtcgtt cgacgaccac agcagcagcg cgaagaagcc ggcgaagccg agcaggtagc
     1501 cgaacgccgc gcgtcccgtc ggcggcccga ggtccttgcg cagcacgcgc agcggcggca
     1561 cgtgtcgcaa ctgcgccagt ggcggcagcg cgaaaccgag cagcagcacg agcccgaccg
     1621 cgccaccctg cagcgccggc gcgaaggaag gcagcggcag ttgcgtctgg atcagcgtgc
     1681 gcagcacctc gagcagcagg aagtgcgcgc cgtagccgac ggccatgccg acggcgcacg
     1741 cggccgcccc ggtgatcacg aactccagcg tgaacagcgc gaagatgtcg cgctgcgcca
     1801 gcccgaggca gcgcatcatc gcgcacgagt cgaggtggcg cagcgagaag cggcgcgcag
     1861 ccgccgccac cgcgaccgcg gcgatcatgg ccgccagcaa cgccaccagc gcgaggaagc
     1921 gttcggcgcg ctcgatcgtg cgctgcatct cggggcggcc gccttcgagc gattcgatgc
     1981 gctggccgcg cacgaggttc tcgccgaccc aggcggcgta cgcgcgcacg acggcctcgt
     2041 tgcctgcgac cagcaggcga tacgtgacgc ggctgccttc ctggatcagt tgcgtcgacg
     2101 gcaggtcggc gatgttcagc aggacgcgcg gcgcgaagtt gatgaactgc gcgccgcggt
     2161 cgggctcgat cgtgatgacg cggtcgatcc ggaaactgcg ctctcccagg cgcaagcggt
     2221 cgccgaccgc cagccgcagc gcctgcagca gcggcgcgtc gacccacacc gtgcccggcg
     2281 cgggaatgtc gcgcgtgacc gcgtccggcg catcgggctg cgtggtcacg cgcagtgcgc
     2341 cgcgcagcgg atacccagtc gacaccgcct tgacagccgc gagcgcggtg tcgttcgcgt
     2401 cgcgctcgct caccgccatg ctcggaaaca cgatcgtttc cgccgtctgc aatccggcgc
     2461 ctcgtgcgcg cgcgcgttcg gcgtcggcga tcggacggtc ggagctgagc acgaggtcgg
     2521 cgccgagcag ttgtttcgcg tcgcgctcca gtccgagccg gatgcg
//
LOCUS       DBNHBY010000706         2924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1810996, whole genome shotgun sequence.
ACCESSION   DBNHBY010000706 DBNHBY010000000
VERSION     DBNHBY010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1810996"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16HB_16755"
     CDS             <1..188
                     /locus_tag="AC16HB_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5608493.1"
                     /translation="RIADGAPLTARWHKKFARRLLDPRPLTDAERDECFDCFDTEDFR
                     IGYRAFLAKEKPRFVGR"
     gene            192..1403
                     /locus_tag="AC16HB_16760"
     CDS             192..1403
                     /locus_tag="AC16HB_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5608494.1"
                     /translation="MQAAKSGPLAGMRVLELTQIMSGPTCGLMLADLGADVIKVEKLP
                     DGDDARRYSEPRVNGVSAPFLMLNRNKRGIALDLKKAEGRAVLLRMVREADVLVENFR
                     TGTMDKLGLGYDVLATQNAGLIYCAITGYGRTGPFADKGGFDLLAQGFSGLMSITGEP
                     HGAPVKSGPPLTDIGAGVLGATGIIAAYVHKLKTGEGQVVDTSLMEAGLQQTYWHAAI
                     FLATGQSPAPTGSAHVLTAPYQAFHASDGWINIGGANQSNWERIAEVLGHPEWKTDPR
                     FATNTDRMANLDALVERMSAVLITRTRAEWIDAFDAAGVPAGPVHSIGEALTHPQTLA
                     RGMVVDVMHPQAGATKGVGCPLHFSRMPPAEPVAAPLLGQHTREVLQEFGYSAKEIDG
                     LIERGVVAESG"
     gene            1438..1941
                     /gene="purE"
                     /locus_tag="AC16HB_16765"
     CDS             1438..1941
                     /gene="purE"
                     /locus_tag="AC16HB_16765"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR5608495.1"
                     /translation="MTKAKQSAPLVGVVMGSNSDWDTMQHACDVLKEFGVAFEAKVLS
                     AHRMPDEMFDYAEHARERGLRAIIAGAGGAAHLPGMLASKTVIPVLGVPVPSKYLNGR
                     DSLLSIVQMPKGVPVATFAIGESGAANAALFAVAMLAAQDNELAMKLAQYRATQTQAA
                     KNMALPL"
     gene            2419..2625
                     /locus_tag="AC16HB_16770"
     CDS             2419..2625
                     /locus_tag="AC16HB_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5608496.1"
                     /translation="MKFSIECEREEDGRWLAEVPELPGVLAYGTTPDEAQGKAQVLAL
                     RVLAERLEHGEARPVDISISVATV"
     gene            2622..2834
                     /locus_tag="AC16HB_16775"
     CDS             2622..2834
                     /locus_tag="AC16HB_16775"
                     /inference="COORDINATES: protein motif:HMM:NF019540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5608497.1"
                     /translation="MSQWPSTKATRVLSALQRIGWQIKRQSGSHKTLSRQGWPDFVFA
                     FRDREEIGPRMLSRIAKRTGLSPKDL"
BASE COUNT          527 a          941 c          975 g          481 t
ORIGIN      
        1 agcgcatcgc cgacggcgcg ccgctcaccg cgcgctggca caagaagttc gcgcgccggc
       61 tgctcgaccc gcgtccgctc acggatgccg aacgcgacga atgcttcgac tgcttcgaca
      121 ccgaggattt ccgcatcggc taccgcgcct tcctggcgaa ggagaagccg cggttcgtgg
      181 ggcgctgaac gatgcaggca gcgaagtccg gcccgctcgc cggcatgcgc gtgctcgaac
      241 tcacgcagat catgtcgggg ccgacctgcg gactgatgct cgcggatctc ggcgccgacg
      301 tgatcaaggt cgagaaactg cccgatggcg acgatgcgcg ccgctacagc gagccgcgcg
      361 tcaacggcgt gtcggcgccg ttcctgatgc tcaaccgcaa caagcgcggc atcgcgctcg
      421 acctgaagaa ggccgaaggc cgcgccgtgc tgctgcggat ggtgcgcgag gccgacgtgc
      481 tggtcgagaa cttccgcacc ggaacgatgg acaagctcgg cctcggctat gacgtgctgg
      541 caacgcagaa cgcggggctg atctactgcg cgatcaccgg ctacggtcgc accggcccat
      601 ttgccgacaa ggggggcttc gatctgctcg cgcagggctt ctccggcctg atgagcatca
      661 ccggcgagcc gcacggcgca ccggtgaagt ccggcccgcc gctgaccgac atcggggccg
      721 gtgtgcttgg ggcgaccggg atcatcgccg cgtacgtgca caagctgaag acgggcgaag
      781 ggcaggtcgt cgacacctcg ctgatggagg cggggctgca gcagacctac tggcacgcgg
      841 cgatcttcct cgcgaccggg caatcgcccg cgccaacggg ttccgcgcat gtgctgaccg
      901 cgccctatca ggcattccac gccagcgacg gctggatcaa catcggcggc gccaaccagt
      961 cgaactggga gcgcatcgcc gaggtgctgg gtcacccgga gtggaagacc gacccgcgct
     1021 tcgccaccaa taccgaccgc atggcgaacc tcgacgcgct ggtcgagcgg atgagcgcgg
     1081 tgctgatcac gcgcacgcgc gcggagtgga tcgacgcgtt cgacgccgcc ggcgtgccgg
     1141 ccggtcccgt gcactcgatc ggcgaggcgc tcacgcaccc gcagacgctg gcgcgcggga
     1201 tggtggtcga cgtgatgcat ccgcaggcgg gcgcaacgaa aggggtgggc tgcccgctgc
     1261 acttttcgcg catgccgccg gcagagccgg tggcagcgcc gctgctcggc cagcacacgc
     1321 gcgaggtgct gcaggagttc ggatattccg ccaaggagat cgacggcctg atcgagcgcg
     1381 gcgtcgtcgc cgagagcggc tgaaacacta caatccgcgc tttgctttcc cgctgacatg
     1441 accaaagcaa agcagtccgc gccgctggtc ggcgtggtga tgggatccaa ctccgactgg
     1501 gacacgatgc aacacgcctg tgacgtcctg aaggagttcg gcgtcgcgtt cgaggcgaag
     1561 gtgctgtccg cgcaccgaat gcccgacgag atgttcgact acgcggagca tgcgcgcgag
     1621 cgcgggctgc gggccatcat cgccggtgcg ggcggcgccg cacacctgcc gggcatgctg
     1681 gcgtcgaaga ccgtgatccc ggtgctcggc gtgccggtgc cgtcgaagta cctgaacggt
     1741 cgcgactcgc tgctgtcgat cgtgcagatg cccaagggcg ttcccgtggc gacgtttgcg
     1801 atcggcgaaa gcggcgccgc caacgcggcg ctgttcgcgg tggcgatgct tgctgcgcag
     1861 gacaacgagt tggcgatgaa gctcgcgcag taccgcgcca cgcagaccca ggcggcgaag
     1921 aatatggcgt tgccgctttg actcgtgatc caactagagg tacgtatagc gatgggtatc
     1981 gcccgatcga atcgtcctga aagggcagcc aagactgaac gaatgatggg tacaggatcg
     2041 gttcgctgag gatcgctaac acgcgaaatg cgttcgacgc gccgccgatt gccgaaggcg
     2101 cgctggccat ccgcgacgat atgcccttcg gagacacgcg cgtgacgatc gatgatctgc
     2161 tgcggcaagg tttgccgtgc caatgcagaa ggtttggtgc cacaacagct tcgtgatgtc
     2221 atcaaatctc ggtatccgag taacgcaagt cgaaacgcgt aactactcgg aactcgagcg
     2281 gtccattcaa aagttgctcg accccttccg cagcaacgga tctcgcgact cctgttcctt
     2341 ggcctggctc actgcgaacg tgcggcacga cgcgctcgga ctcattagaa tccctttgcc
     2401 tcaaccagtg gggacgcgat gaagttcagc atcgagtgtg aacgcgaaga agacggccgt
     2461 tggctggccg aggttccgga gctgccgggt gtcctcgcct acggaaccac gcccgacgag
     2521 gcgcagggca aggcccaggt gctggcgctg cgcgtcctcg ccgagcgcct tgagcatggc
     2581 gaggcacgtc cggtcgacat cagcatctcc gttgctacgg tatgagccag tggccttcga
     2641 cgaaggccac tcgtgttctc tcggcccttc aacgcatcgg gtggcagatc aagcgccaat
     2701 cgggatccca taagacgctc tcgcgccaag gctggcccga ttttgtcttt gcatttcgtg
     2761 atcgcgaaga aatcggacct cgaatgcttt cccgtatcgc gaagcgcacc ggcctcagtc
     2821 cgaaggacct gtaggtaagt gacttgaacc ttcaacctgg cacctcgaac ctgatgtcga
     2881 tctcccccgg cgattggctc ggcctgctcg gcggcgggca gctc
//
LOCUS       DBNHBY010000707         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_797504, whole genome shotgun sequence.
ACCESSION   DBNHBY010000707 DBNHBY010000000
VERSION     DBNHBY010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_797504"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16HB_16780"
     CDS             <1..231
                     /locus_tag="AC16HB_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5608498.1"
                     /translation="LTERRIDPQRYKRVYLHMIGAEDAMEAYGQASKYDTAPDFLAEL
                     FALGRQTAQEWLEMRYEYIGHAGTVSIAETFL"
     gene            277..>1586
                     /locus_tag="AC16HB_16785"
     CDS             277..>1586
                     /locus_tag="AC16HB_16785"
                     /inference="COORDINATES: protein motif:HMM:NF021606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HIR5608499.1"
                     /translation="MIAWGVFWGALFGLVSGHFDGASMILGAAIGALAGGSLRRAVDR
                     RIAEKLTEPKVKARDVVAPAPAVKVQAAAPITPPEPVSAVVPPPASAQPLEASPPTIP
                     LQPVARAPRSLSAPELVVERIKAWLLGGNTVARLGALVFFIGLAFLARFAAEQGLFPP
                     QLRLAGIGLVAIVLLVLGFRLRERRSGYALTLQGVGVAILYLTIFAALRLYGFIAPLP
                     AFALMVAVCALSAMLAVLQDSRALAVISAAGGFVTPILVSTGQGDHVALFSYYTVLNF
                     GILGIAWTKDWRPLNLVGFVFTFGIATLWGVLRFMPANYTSAQPFLVVFFVQYVAIAV
                     LFALRRAPRLTDYVDATLVFGVPLIGFGLQAGLVRHFEYGLAWSAIAVAAFYLGLAWL
                     LAQRRLERLRLLVECFIALGVIFASLAIPFALDARWTAAAWALEG"
BASE COUNT          194 a          506 c          581 g          305 t
ORIGIN      
        1 ctgacggaga gacgcatcga cccgcagcgc tacaagcgcg tctacctgca catgatcggt
       61 gcggaggacg cgatggaggc gtacggccag gcgagcaagt acgacacggc gccggacttc
      121 ctcgccgagt tgttcgcgct gggacgtcag accgcccagg aatggctgga gatgaggtac
      181 gagtacatcg gccacgcggg aacggtaagc atcgcggaga cgttcctgta gcagattgcc
      241 aagggtgctg ggagcgccgg aggtccgggg gagacgatga tcgcgtgggg cgttttttgg
      301 ggcgcgctgt tcggcctggt ctcggggcat ttcgacggcg cgagcatgat cctgggcgcg
      361 gcgatcggcg cgctcgccgg cgggtcgctg cggcgggcgg tggatcggcg cattgcggag
      421 aagctgaccg agcccaaggt caaagcgcgc gacgtcgtgg cgccggcgcc ggccgtcaag
      481 gttcaggcgg ctgcgccgat cactccgcct gagcccgtct ctgcagtggt gcccccgccg
      541 gccagcgctc aaccgctcga agcatcgccg ccaacaatcc cgctgcagcc ggttgcacgt
      601 gcgccacgtt cgctctcggc gccggaactc gtcgtcgagc gcatcaaggc gtggcttctg
      661 ggcggcaaca cggtcgcgcg gctcggcgcg ctggtgttct tcatcgggct tgccttcctc
      721 gcgcgcttcg ccgccgagca gggattgttc ccgccgcaac tgcggctcgc cggcatcggc
      781 ctcgtggcga tcgtcctgct cgtgctcggc ttccggctgc gcgaacggcg cagcggctac
      841 gcgctgacgc tgcagggcgt cggtgtggcg atcctgtacc tgacgatctt cgccgcgttg
      901 cggctgtacg ggtttatcgc gccgctgccg gcgttcgcgc tgatggttgc ggtgtgtgcg
      961 ctgtcggcga tgctcgcggt gctgcaggat tcgcgcgcgc tggcggtgat cagcgccgcc
     1021 ggcggcttcg tgacgccgat cctcgtttcg accgggcagg gcgatcacgt ggcgctgttc
     1081 agttactaca cggtgctgaa tttcgggatc ctcggcatcg cgtggacgaa ggactggcgg
     1141 ccgcttaacc tggtcggctt cgtattcacg ttcggcattg ccacgctgtg gggggtgctg
     1201 cgcttcatgc ccgcgaacta cacgagcgcg cagccgttcc tggtcgtgtt cttcgtgcag
     1261 tacgtcgcga tcgcggtgct gttcgcgctg cgccgcgcgc cgaggctgac cgactacgtc
     1321 gacgccacgc tcgtattcgg cgtgccgttg atcggcttcg gcctgcaggc ggggctggtg
     1381 cggcacttcg agtacgggct cgcgtggtcg gcgattgcgg ttgcggcctt ctatctggga
     1441 cttgcctggc tgctcgcgca gcgccggctc gagcgattgc ggctgctggt cgagtgcttc
     1501 atcgcgctcg gcgtgatctt cgcgtccctg gcgattccgt tcgcgctcga cgcgcgctgg
     1561 accgccgccg cgtgggcgct ggaagg
//
LOCUS       DBNHBY010000708         2913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1608359, whole genome shotgun sequence.
ACCESSION   DBNHBY010000708 DBNHBY010000000
VERSION     DBNHBY010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1608359"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC16HB_16790"
     CDS             <1..458
                     /locus_tag="AC16HB_16790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR5608500.1"
                     /translation="ADGRLLPFSNLLDLLARLHRGQVKEAPCGAGLGYLALDAEGRFF
                     LCHRLAGADRFRVGDLDRGIDHDRIRACLAAQAAPRQDACRTCWARGLCAGGCHHDNH
                     VRENQLGLAPGGSCDFIRRWIEIGIGTYADLVRDPDHRLLAFLARRADG"
     gene            482..808
                     /gene="qhpC"
                     /locus_tag="AC16HB_16795"
     CDS             482..808
                     /gene="qhpC"
                     /locus_tag="AC16HB_16795"
                     /inference="COORDINATES: protein motif:HMM:NF037958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinohemoprotein amine dehydrogenase subunit
                     gamma"
                     /protein_id="HIR5608501.1"
                     /translation="MKKKLIEALNEKAKAIERLADGEQATAEALGKLPAMPQGCATIF
                     DTGWETNPRPTYPIGNCQASVRDFHGCAGDCWWPAQVPDGLTNHKDFDVACPNVARDW
                     RNLKYD"
     gene            818..1831
                     /locus_tag="AC16HB_16800"
     CDS             818..1831
                     /locus_tag="AC16HB_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5608502.1"
                     /translation="MAWKRLLPLALVAIACVVGTPRAEARDRLYHLGYEIIQVIDGET
                     DAIVADIPIKGAARDVGLTADKKFLYVATNRHLVHKVDLAENRVVGSIDLNSDDWERF
                     MFGFALDADGKTAYGAMMSRTAKNGEAVVGKPVVAQFDLATGKLLRSVEVPWGVAHLV
                     GVQGGRMIYAFGQDLYKIDTTGPEMKIVETVPMFDKEMNILPFWDYAFDNGGVASMNY
                     YTPKAMGLLLVDQATGAVQDILLRGEPVMAYSVVLSPDRRKAYAVMDDLNVIDLEKKT
                     YVATVPIKEGTCYGVNVSSDGKKIYVGGGGSTLTVFDARTLKPLKVLQMASDGMDLRR
                     ISF"
     gene            1837..2664
                     /locus_tag="AC16HB_16805"
     CDS             1837..2664
                     /locus_tag="AC16HB_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608503.1"
                     /translation="MEYGLRLARPVPPEADPRDACPAPWRHMLPEGPAVALYFGSEFC
                     EDRLPGFDEAQSFCALARERGWEPTLLTPLATGAGLGIVHRLLAGLEADGWTPAVVFN
                     DWGVLGLLREQHPSLPRRAGRLINRSLRDPRAYRDAPAGSATHDGSRFSRLRQFLSDA
                     GVSAVETDADLEGGYLGDGSEGLARALHFPFTFAASGRNCPLKALLYPEGRGFSKSLA
                     DPCTAPCRSGPAPVQRHDTTLPHWRGGNTLFYEIPQDAARAWLAHADRIVLHEEAAP"
     gene            2661..>2913
                     /locus_tag="AC16HB_16810"
     CDS             2661..>2913
                     /locus_tag="AC16HB_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608504.1"
                     /translation="MKVLAPLRNSDEVPALIDAGAQEFYCGLTPPGWEQSFGHAPVHR
                     RNARSAGVPSLEDLRRIVALARPRPVYATLNAPSFPAGAV"
BASE COUNT          467 a          963 c         1020 g          463 t
ORIGIN      
        1 ccgccgacgg ccgcctgctg ccgttttcca atctgctcga tctgctcgcg cgactgcatc
       61 ggggacaggt gaaagaggcg ccgtgcggcg ccgggctcgg ctatctcgcg ctcgacgccg
      121 aaggccgttt cttcctgtgc caccggctgg ccggtgccga ccgcttccgc gtcggcgatc
      181 tcgatcgcgg gatcgaccac gaccggatcc gcgcctgcct cgccgcgcag gcggcaccgc
      241 gccaggatgc ctgccgcacg tgctgggcgc gcggcctgtg cgcgggcggc tgtcatcacg
      301 acaaccatgt gcgcgagaac caactggggc tcgctccggg cgggtcgtgc gacttcatcc
      361 ggcgctggat cgagatcggc atcggcacgt atgccgatct cgtccgcgat cccgaccacc
      421 gcttgctcgc cttcctcgcg aggcgggccg acggctgaga actttccgac aggagatggc
      481 gatgaaaaag aaactcatcg aggcattgaa cgaaaaggcc aaggccatcg agcgcctcgc
      541 cgacggcgag caggcgaccg ccgaagcgct gggcaagctg cccgcgatgc cgcagggctg
      601 cgcgacgatc ttcgatacgg gctgggagac caatccccgg ccgacctacc cgatcgggaa
      661 ctgccaggcg tcggtgcgcg actttcacgg ctgcgcgggc gattgctggt ggcccgcgca
      721 ggtgccggac gggctcacca accacaagga tttcgacgtc gcctgcccga acgtcgcgcg
      781 cgactggcgc aacctgaaat acgactagga ggacgacatg gcgtggaagc ggcttctgcc
      841 tctggccctg gttgcgatcg cgtgcgtcgt cgggacgccg cgcgccgagg cgcgcgaccg
      901 gctctatcac ctcggctacg agatcatcca ggtgatcgac ggcgagaccg atgcgatcgt
      961 cgccgacata ccgatcaagg gcgcggcccg cgatgtcggc ctgacggcgg acaagaaatt
     1021 cctgtacgtg gcgaccaacc ggcatctggt gcacaaggtc gatctcgccg agaaccgcgt
     1081 cgtcggcagc atcgatctga acagcgacga ctgggaacgc ttcatgttcg gcttcgcgct
     1141 cgatgccgat ggcaagacgg cctacggcgc aatgatgtcg cgcaccgcga agaacggcga
     1201 agccgtggtg ggcaagccgg tggtggcgca gttcgatctt gccaccggca agctgctgcg
     1261 cagcgtcgag gtgccatggg gcgtggcgca cctcgtcggc gtccagggcg ggcggatgat
     1321 ctatgcgttc ggccaggacc tgtacaagat cgacacgacc ggacccgaga tgaagatcgt
     1381 cgagacggtg cccatgttcg acaaggagat gaacatcctg ccgttctggg attacgcgtt
     1441 cgacaacggt ggcgtggcca gcatgaacta ctacacgccg aaggccatgg ggctgctgct
     1501 ggtcgatcag gcgaccggcg cggtacagga catcctgctg cggggcgaac cggtgatggc
     1561 gtattcggtg gtgctgtcgc ccgaccgcag gaaggcctac gcggtgatgg acgacctgaa
     1621 cgtcatcgac ctcgagaaga agacgtacgt tgcgacggtg ccgatcaagg aaggcacgtg
     1681 ctacggcgtc aacgtgtcga gcgacgggaa gaagatctac gtcggcggcg gcgggtcgac
     1741 attgacggtg ttcgacgcgc ggacgctcaa gccgctcaag gtgctgcaga tggcatccga
     1801 cggcatggat ctgcgccgga tcagcttcta acggccatgg aatacgggct gcgactggcg
     1861 cgcccggttc caccggaggc ggatccgcgc gatgcgtgtc cggcgccgtg gcgacacatg
     1921 ctgccggaag gtccggcggt ggcgctgtac ttcgggtcgg agttctgcga agaccggctt
     1981 ccggggttcg acgaggcgca gtcgttctgc gcgctggcgc gcgagcgcgg ctgggagccg
     2041 acgctgctca cgcccctggc aactggcgcc ggactcggca tcgtgcatcg gctcctggca
     2101 ggtctcgaag cggacggctg gacgcccgcg gtcgtcttca acgactgggg ggtgctcgga
     2161 ctgctgcggg aacagcaccc gtcgctgccg cggcgcgcgg gccggctgat caatcgctca
     2221 ctacgcgatc cgcgcgcgta tcgcgatgct ccggccggat ctgcgaccca cgacggctcg
     2281 cgcttttcgc gcttgcggca gttcctgtcc gatgccggcg tgtccgcagt ggagaccgat
     2341 gcggatctcg agggcggcta tctcggcgac ggcagcgaag ggctggcgcg ggcgctgcac
     2401 tttccattca cgttcgcggc atccggccgc aactgcccgc tgaaggcatt gctgtatccg
     2461 gagggtcgcg gcttctccaa gtcgctcgcc gatccttgca cggcgccgtg ccgctcgggg
     2521 ccggcgccgg tgcagcgcca cgacacgacg cttccgcact ggcgcggcgg caacaccctc
     2581 ttctacgaga tcccgcagga cgccgcgcgc gcgtggcttg cgcacgcgga ccggatcgtg
     2641 ctgcacgagg aagccgcgcc gtgaaggtgc tcgcgccgct gcgcaactcc gacgaagtgc
     2701 cggcgctcat cgatgccggc gcacaggagt tctactgcgg cctcacgccg cccggctggg
     2761 agcagtcctt cggccacgcg ccggtgcatc ggcgcaatgc acgatcggcc ggcgtgccga
     2821 gcctcgagga cctgcggcgc atcgtcgcgc tcgcacgccc gcgtccggtc tatgcgacgc
     2881 tcaacgcgcc ttcgtttccg gcgggcgcgg tgc
//
LOCUS       DBNHBY010000709         6355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_594289, whole genome shotgun sequence.
ACCESSION   DBNHBY010000709 DBNHBY010000000
VERSION     DBNHBY010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_594289"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="AC16HB_16815"
     CDS             complement(<1..448)
                     /locus_tag="AC16HB_16815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5608505.1"
                     /translation="MKVCVVGAGSIGAVLGAKLAATGHDVCLIARGAHLDAIRANGLK
                     LVDHVDDRSGTYALAASGDPADFGVQDLVVIALKAHSIAAMLPRARPLIGPHTTVVPA
                     INGLPWWYFHREGGAHDGMRVRALDPHGTMFADLDCRHIVGCVVHVA"
     gene            complement(448..2403)
                     /locus_tag="AC16HB_16820"
     CDS             complement(448..2403)
                     /locus_tag="AC16HB_16820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5608506.1"
                     /translation="MSALPASAPYGAWVSPISAARAGAAANALGSVTAHRGRLYWVEE
                     RPAEQGRSVLVTTGDAGDVVELTNSPFNVRTRVHEYGGLAYAHVGARIVFSNFADQLL
                     YALDGAAPPRPLTPPGYRYADGRAAADATLYCVREDHTGGGEPKNTIVALDVNAGISA
                     GRVLYDASDFVAYPRADAEGRRLAFIRWDHPQMPFDATTLLVGAIDAGSLRDLRAIAG
                     SGGAESVLEPQWDGDGSLYFLSDRSGYWNLCRWRADRIDRVTAFDADLGGPLWQLGAS
                     TYALTGDGRALVRLTRNAVDELHLIDLATGHGRRLGLPFVAFGAIGMIDANTAHALAA
                     SSSDLPALIAIDLASGAHRVVRRAGALDLPPDCIARAEAIEFPTAAAPDGTPRTAHAF
                     FYAPRNPNFRAEPGTKPPLIVTLHGGPTSHATPAFAIATQFWTTRGFAVVSVNYGGST
                     GFGRAYRERLRGQWGVLDVQDAVAAVDYLAAGGRVDPRRIAIRGGSAGGFTVLRALAG
                     TDRFTAGINYFGVADAEALVRDTHKFESRYCDSLIAPWPEAIDQYRARSPIRHLDGFR
                     APLITFQGAEDRAVPPSQSRAIVEALRTRGIPVAYLEFAGEQHGFRDARNITRALEAE
                     LYFLGRVFGFAPADRIEPVPIDNLGSE"
     gene            complement(2400..3329)
                     /locus_tag="AC16HB_16825"
     CDS             complement(2400..3329)
                     /locus_tag="AC16HB_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HIR5608507.1"
                     /translation="MNLQQFRFLREAARQKFNITAAARALHTSQPGISKAIIELESEL
                     GVPIFARQGKRILGLTPPGQQILLAAERIMQEVDNLKKLARDYAGAPSGTLRVATTHT
                     QARYVLPTVVQKFARKFPQVRLKLLQGNPPQIAQMLVNGQADVGIATESLAETPGLAC
                     IDIYEWTHAAVVPPGHSLAKFQVSPGKLTLKDLARSPIVTYEAHFAGRTRIDAAFAAA
                     GIEPAIVLEAIDADVIKTYVRAGMGVGIIAGVAADPANEPLIVLPCGHLFGKNVARLA
                     VKQGAYLRGFVYSFIEMLAPGWDKRRIEQAFGQ"
     gene            3606..>6355
                     /locus_tag="AC16HB_16830"
     CDS             3606..>6355
                     /locus_tag="AC16HB_16830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR5608508.1"
                     /translation="MKLDKAQLLLEEQPISIDVLLEKYAKGDEKSIDDVRRRVARGLA
                     VVEKPELRAEWERRFYEAMVDGFIPGGRVNSAAGTEIAATLINCFVQPVGDAISGVNG
                     EGLPSIYLALNQAAETMRRGGGVGYDFSHIRPRGALVKGTHSRASGPLSYMRVFDKSC
                     ETLESAGARRGAQMGMMRCDHPDIEDFIHAKRDGSLSNFNMSVAVTDEFMRAVDADGD
                     IELWHGAEPFDRSNGYQRADGTWVYRKARARELFDQIMQSTYNHAEPGVVFIDRVNQD
                     NNLSYCETIAATNPCGEQPLPNYGCCCLGSINLTRFVNDPFGEGAAFDYARFRDVVKV
                     SIRALDNVLDATLWPLPEQNREAMNKRRVGLGFTGLANTLTMLNLRYDSETGLKVAGD
                     VARAMRDASYEASVDLAKEKGRFPLFDADKFLAAPHCASRLPDSLKAQIRVHGARNSH
                     LLSIAPTGTISLAFAGNASGGIEPTFSWTYIRKKRMPDGSKQEYVVEDYAYRMYRHLG
                     GDEHHLPPSFVHALEMSAFDHLKMIAAVQPYIDAAVSKTVNVPEDYPFEDFKGLYFEA
                     WKAGLKGITTYRPNSVIGSVLETKKKPETQEPQDLKDDPDRRVRLDSAPQPALASLKW
                     PSRPDLSEGNMAWTYMVEVPGRNEKFAIFIGQVENNGNPPMPFEVWVNGAEQPRGLGA
                     IAKALSMDMRSEDRAWLKMKLEALAKARDEQGFMLPMPPRGEMKWQNGVVAAFANIVR
                     WRCEQLGVFRDLEQQLLPMPTPVMNALFSKKEPKAGPRGTMSWTVDVSNANTGDDFVL
                     GVKELQLPDGSRRPYSIWLAGEYPKVLDGLCKVISLDMRVMDPNWIGLKLKKLLNFGE
                     QNGEFWALVPGEEKSTLYPSTVAYIATLLLHRYKVLGILDDEGRAIQSAGILALPDAV
                     KI"
BASE COUNT         1081 a         2144 c         2172 g          958 t
ORIGIN      
        1 ccgcgacgtg caccacgcaa ccgacgatgt ggcggcagtc gaggtcggcg aacatcgttc
       61 catgggggtc gagcgcgcgc acgcgcatgc cgtcgtgcgc gccgccctcg cgatggaagt
      121 accaccatgg cagcccgttg atcgccggca ccacggtcgt gtgcgggccg atgagtggcc
      181 gcgcgcgcgg cagcatcgcc gcgatcgaat gcgccttcag cgcgatcacg accaggtcct
      241 gcacgccgaa gtcggccggg tcgccgcttg ccgccagcgc gtaggtgccg ctccgatcgt
      301 cgacgtggtc cacgagcttc agtccgttcg cgcggatcgc gtccagatgt gccccgcgcg
      361 cgatcaggca gacatcgtga cccgtggccg ccagcttggc gccaagcacc gcgccgatcg
      421 agccggcgcc gacgacgcag actttcatca ctcggaaccg agattgtcga tcggcaccgg
      481 ctcgatgcgg tcggccggcg cgaacccgaa cacgcggccg aggaagtaca gctccgcttc
      541 gagcgcgcgc gtgatgttgc gcgcgtcgcg gaagccgtgc tgctcgcccg caaactccag
      601 gtaagcgacc gggatgccgc gcgtgcgcag cgcctcgacg atggcgcggc tctggctcgg
      661 cggcactgct cggtcctcgg cgccctggaa cgtgatcagc ggcgcgcgga atccgtcgag
      721 gtggcggatc gggctgcgcg cgcgatactg atcgatcgcc tccggccacg gcgcgatcag
      781 gctgtcgcag taacggctct cgaacttgtg cgtatcgcgc accagcgctt cggcatcggc
      841 cacgccgaag tagttgatgc cggccgtgaa gcggtcggtg cccgcgagcg cgcgcagcac
      901 ggtgaagccc ccggcgctgc cgccgcggat ggcgatccgg cgcggatcga cgcgcccgcc
      961 tgcggcaagg tagtcgaccg cggccacggc gtcctggacg tcgagcacgc cccattgccc
     1021 gcgcagccgc tcgcggtatg cgcgtccgaa tccggtcgaa ccgccatagt tgacgctgac
     1081 gacggcaaag ccacgcgtcg tccagaactg cgtggcgatg gcaaaggctg gcgtcgcatg
     1141 cgatgtcggt ccgccgtgca aggtgacgat cagcggcggc ttcgtgcccg gctcggcgcg
     1201 gaagttcgga ttgcgcggcg catagaagaa cgcgtgcgcg gtgcgcggcg taccgtccgg
     1261 cgcggcggcg gtcggaaact cgatcgcctc ggcccgcgcg atgcagtccg gaggcagatc
     1321 gagcgcgccg gcgcggcgca cgacgcgatg cgcgcccgac gcgaggtcga tcgcgatcag
     1381 tgccggcaga tcgctgctcg aagcggcgag cgcatgggcg gtgttggcat cgatcatgcc
     1441 gatcgctccg aacgcaacga aagggagccc gagccgtcgg ccgtggccgg tggcgagatc
     1501 gatcaggtgc agttcgtcga ctgcgttgcg cgtgaggcgc accaacgcgc gtccgtcgcc
     1561 cgtcagcgcg tacgtagacg cgccgagctg ccacagcggt ccgccgagat cggcgtcgaa
     1621 cgccgtcacg cggtcgatgc ggtcggcgcg ccaccgacac agattccagt agccgctgcg
     1681 atcggacagg aagtacagac tgccatcgcc gtcccattgc ggctcgagca ccgactcggc
     1741 cccgccgctg cccgcgatcg cgcgcagatc gcgcaggctg ccggcgtcga tcgcgccgac
     1801 cagcagcgtc gtcgcgtcga acggcatctg cgggtgatcc cagcggatga aggcgagccg
     1861 gcgcccctcg gcatcggcgc gcggataggc gacgaagtcg gacgcgtcat acagcacgcg
     1921 tccggccgag atgcccgcgt tcacgtcgag cgcgacgatc gtgttcttcg gttcgccgcc
     1981 gccggtgtga tcttcgcgca cgcagtagag cgtggcgtcg gccgcggcgc gtccgtcggc
     2041 atagcggtag cccggcggcg tcagcgggcg cggcggcgcc gcgccgtcga gcgcgtacag
     2101 caactggtcg gcaaaattgc tgaacacgat gcgcgcgccg acatgcgcgt atgcaagtcc
     2161 gccgtattcg tgcacgcgcg tgcggacgtt gaagggggaa ttcgtcagct cgaccacgtc
     2221 gccggcgtcg ccggtcgtga cgagcacgct gcgcccctgt tccgccggcc gctcctcgac
     2281 ccagtacaag cgcccgcggt gcgcggtgac gctgcccagc gcgttggccg ccgctcccgc
     2341 gcgcgcggcg ctgatcggcg agacccacgc gccgtacggc gcgcttgcag gcagcgcgct
     2401 cattgcccga acgcctgctc gatccggcgc ttgtcccacc cgggcgcgag catctcgatg
     2461 aacgagtaca cgaagccgcg caggtaggcg ccctgcttga ccgccaggcg cgcgacattc
     2521 ttgccgaaca agtggccgca cggcagcacg atcagcggct cgttggccgg atcggccgcc
     2581 acgccggcga tgatgcccac ccccatgccg gcgcgcacat aggtcttgat cacatcggcg
     2641 tcgatcgcct ccagcacgat cgcgggctcg atgcccgccg cggcgaacgc cgcgtcgatg
     2701 cgcgtccggc cggcgaagtg cgcctcgtac gtgacgatcg gactgcgcgc caggtccttc
     2761 agcgtcagct tgccgggcga gacctgaaac ttcgccagcg aatgccccgg cgggaccacg
     2821 gccgcgtgcg tccattcata gatgtcgata cacgccagcc ccggcgtttc tgccagcgat
     2881 tcggtcgcga taccgacgtc ggcctgcccg ttgacgagca tctgcgcgat ctgcggcgga
     2941 ttgccctgca gcagcttcag ccgtacctgc ggaaacttgc gcgcaaactt ctgcaccacc
     3001 gtcggcagca cgtagcgcgc ctgcgtgtgc gtggtcgcca cgcgcagagt gcccgaaggc
     3061 gcgcccgcat aatcgcgcgc cagcttcttc aggttgtcga cctcctgcat gatgcgctcg
     3121 gccgcgagca ggatctgctg gccgggcggc gtgagaccca ggatgcgctt gccctggcgc
     3181 gcgaagatcg gcacgccgag ctcgctctcg agttcgatga tggccttgga gatgccgggc
     3241 tgcgacgtgt gcagcgcccg cgctgccgcg gtgatgttga acttctggcg cgccgcctcg
     3301 cgcagaaagc ggaactgctg caggttcatc gccggctcct cgtttccgcc gctccgacgc
     3361 ggcacagtat aggggcgcaa aagcaagatt ccggcccgcc cgccgcaatc cgcgaaatgc
     3421 gctagatgct gcgttgcttt gacgaacaac ctcaatatat tgtgttgtta gcgcagatca
     3481 aaatctatgc tggcaacaaa gtttttctgc acgcggcagc ggaagttcct atactggccg
     3541 ctcgaaattc gatgcgacgt ccgcgcgcaa ccgcacgcgc ggaaccgaaa agaggagcca
     3601 tacgcatgaa gctggacaag gcgcaactgc tgctcgaaga gcagccgatc tcgatcgacg
     3661 tgctgctcga gaagtacgcc aagggcgacg agaaatcgat cgacgacgtg cgccgccgcg
     3721 tggccagggg gcttgccgtg gtcgagaagc cggaactgcg cgcggagtgg gaacgccgct
     3781 tctacgaggc gatggtcgac ggcttcattc cgggcggtcg cgtgaattcc gccgccggca
     3841 ccgagattgc cgcgacgctg atcaactgct tcgtgcagcc ggtgggcgac gcgatcagcg
     3901 gcgtcaacgg cgaggggcta ccgtcgatct acctggcgct gaaccaggct gccgagacga
     3961 tgcggcgcgg cggcggcgtg ggctacgact tctcgcacat ccgcccgcgc ggcgcgctgg
     4021 tcaagggcac gcactcgcgg gcgagcggcc cgctctccta catgcgcgtg ttcgacaaga
     4081 gctgcgagac gctcgaatcc gccggcgcgc ggcgcggcgc gcagatgggc atgatgcgct
     4141 gcgatcaccc ggatatcgaa gacttcatcc acgccaagcg tgacggctcg ctgtcgaact
     4201 tcaacatgag cgtggcggtg accgatgagt tcatgcgcgc ggtggatgcc gacggcgaca
     4261 tcgagctgtg gcacggcgcc gagccgttcg accgcagcaa cggctaccag cgcgccgacg
     4321 gcacgtgggt ctatcgcaag gcgcgcgcgc gcgagctctt cgatcagatc atgcaatcga
     4381 cttacaacca cgccgagccc ggcgtggtgt tcatcgaccg cgtgaaccag gacaacaacc
     4441 tgtcctactg cgagacgatc gcggcgacca atccgtgcgg cgagcagccg ctgccgaact
     4501 acggctgctg ctgtctgggg tcgatcaacc tgacgcgctt cgtcaacgat ccattcggcg
     4561 aaggcgcggc gttcgactac gcgcgcttcc gcgacgtcgt caaggtttcg atccgcgcgc
     4621 tcgacaacgt gctcgacgcc acgctgtggc cgctgccgga acagaaccgc gaagcgatga
     4681 acaagcgccg cgtcgggctc ggcttcaccg ggctcgccaa cacgctgacg atgctgaacc
     4741 tgcgctacga ctcggagacc gggctgaagg tcgcaggcga tgtcgcgcgc gcgatgcgcg
     4801 acgcctcgta cgaggcctcg gtcgatctcg cgaaggagaa gggacgcttc ccgctgttcg
     4861 acgccgacaa gttcctggcc gcgccgcact gcgcgtcgcg gctgcccgac agcctcaagg
     4921 cgcagatccg cgtgcacggc gctcgcaact cgcacctgct gtcgatcgcg ccgaccggaa
     4981 cgatctcgct cgcattcgcc ggcaatgcgt cgggcggcat cgagccgacg ttctcgtgga
     5041 cctacatccg caagaagcgg atgcccgacg gcagcaagca ggaatatgtg gtcgaggact
     5101 acgcgtaccg catgtaccgc cacctcggcg gcgacgagca ccacctgccg ccgtcattcg
     5161 tgcacgcgct ggagatgagc gcgttcgacc acctgaagat gatcgctgcc gttcagccgt
     5221 acatcgacgc cgctgtcagc aagacggtga acgtgcccga ggactatccg ttcgaggact
     5281 tcaagggcct gtacttcgag gcatggaagg cggggctgaa gggcatcacc acgtatcgcc
     5341 ccaacagcgt gatcggttcc gttctcgaga ccaagaagaa acccgagacg caggagccgc
     5401 aggacctgaa ggacgatccc gatcgccgcg tgcggctcga ctccgcgccg cagccggcct
     5461 tggcgagcct gaagtggccg agccgtccgg atctgtccga aggcaacatg gcctggacct
     5521 acatggtcga ggtgccgggc cgcaacgaga agttcgcgat cttcatcggc caggttgaaa
     5581 acaacggcaa cccgccgatg ccgttcgagg tctgggtcaa cggcgccgag cagccgcgcg
     5641 gcctgggcgc gatcgccaaa gcgctttcga tggacatgcg cagcgaggac cgcgcgtggc
     5701 tgaagatgaa gctcgaggcg ctggccaagg cgcgcgacga gcagggcttc atgctgccga
     5761 tgccgccgcg cggcgagatg aagtggcaga acggcgtggt ggccgcgttc gccaacatcg
     5821 tgcgctggcg ctgcgagcaa ctcggcgtgt tccgcgacct cgagcagcaa ctgctgccga
     5881 tgcccacgcc ggtgatgaac gcattgttca gcaagaagga gccgaaggcc gggccgcgcg
     5941 gcacgatgag ctggaccgtg gacgtgtcca acgccaacac cggcgacgac ttcgtgctcg
     6001 gcgtcaagga actgcagctg ccggacggca gccgccgccc ctattcgatc tggctcgccg
     6061 gcgagtaccc gaaggtgctc gacggactgt gcaaggtgat ctcgctcgac atgcgcgtga
     6121 tggacccgaa ctggatcggc ctgaagctga agaagctgct caacttcggc gagcagaacg
     6181 gcgagttctg ggcgctggtg ccgggcgagg agaagtcgac gctgtacccg agcacggtgg
     6241 cctatatcgc cacgctgctg ctgcaccgct acaaggtgct gggtatcctc gacgacgagg
     6301 gccgcgcgat ccagagtgcg ggcattctgg cgctgcccga cgcggtgaag atcat
//
LOCUS       DBNHBY010000710         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_458035, whole genome shotgun sequence.
ACCESSION   DBNHBY010000710 DBNHBY010000000
VERSION     DBNHBY010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_458035"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /gene="ribD"
                     /locus_tag="AC16HB_16835"
     CDS             complement(<1..1111)
                     /gene="ribD"
                     /locus_tag="AC16HB_16835"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5608509.1"
                     /translation="MPDYSAVDHQHMARALQLASLGRCSAAPNPAVGCVVIDASGAVV
                     GEGWHRRAGEPHAEVLALRAAGDRAGGGTLYVTLEPCVHHGRTPPCVDAVLASGVARV
                     VAAMSDPNPRVAGGGLQRLAAAGIVTQSGLLAADARELNRGFIARMTRGRPWVTVKLG
                     QSADGRTALADGTSQWITGEAARADVQRLRGRACVVLTGIGTVLADDPALTVRDAALP
                     LHGRQVRRAVLDTHGRLPPQARVASDGVPTLHFTARDGSAKRVDTAPGGSLSVRTAAT
                     NAAGRLDLADVLAQLAELECNEVLVEAGPTLAGAFVHPGLADELVLYVAPTVLGHDGR
                     PTLQWPESLRSLAERPRFAFHDVRRVGADLRLTLRP"
     gene            complement(1111..1635)
                     /gene="nrdR"
                     /locus_tag="AC16HB_16840"
     CDS             complement(1111..1635)
                     /gene="nrdR"
                     /locus_tag="AC16HB_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5608510.1"
                     /translation="MYCPFCAHEETKVIDSRLAGEGQQVRRRRECLRCGERFTTFESA
                     ELVMPRVIKSDATREPFDESKLRSGMLKALEKRPVASEAIDAAVNHVCHRLRTLGERE
                     VPARLIGELVMDELHQLDEVAYVRFASVYRSFQDVDAFREEIERLRHRRPRSVEDLQL
                     GLWSGADPDAGDPK"
     gene            complement(1692..>2148)
                     /locus_tag="AC16HB_16845"
     CDS             complement(1692..>2148)
                     /locus_tag="AC16HB_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR5608511.1"
                     /translation="VIAAKAVAFLEAMQPEFVDYQKQVVANAREMCRVLQERGHRIVS
                     GGTDNHLFLLDLIDKTYTGKDADAALGRAHITVNKNAVPNDPRPPFVTSGLRIGTPAV
                     TTRGFGAAEVSQLAHWIADVLVDVNDEAVVTRTREATRELCRRFPVYGS"
BASE COUNT          296 a          764 c          773 g          315 t
ORIGIN      
        1 cgggccgcag cgtcaggcgc aggtcggccc cgacccggcg cacatcgtgg aaagcgaaac
       61 gcggccgctc ggcgagcgag cgcagcgact ccggccactg cagcgtgggc cggccgtcgt
      121 gccccaacac ggtcggcgcg acatacagca cgagttcatc cgcgagccct gggtgcacga
      181 acgcgcccgc cagcgtcggc cctgcttcga cgagcacctc gttgcactcc agctccgcga
      241 gttgcgccag cacatcggcc agatcgagcc ggcctgcggc attcgttgca gccgtacgca
      301 cgctcagcga gccgccgggt gcggtgtcaa cgcgctttgc gctgccgtcg cgagccgtga
      361 agtgcagcgt gggcactccg tcgctggcca cgcgcgcctg cggcggcagt cgcccgtgcg
      421 tgtcgagcac ggcgcggcgc acctggcggc cgtgcagcgg cagggccgca tcgcgcacgg
      481 tcagtgcggg atcgtcggcg aggacggtgc cgatgccagt cagcacgacg caggcccgcc
      541 cgcgcaggcg ctgcacgtct gcgcgcgctg cctcgccggt gatccattgg ctggtgccgt
      601 cggcgagcgc ggtgcgaccg tccgcactct ggcccagctt gacggtcacc cacggccggc
      661 cgcgcgtcat gcgcgcaatg aaaccgcggt tgagttcacg cgcatccgct gcgagcaatc
      721 cgctctgcgt gacgatgcca gcggcggcca ggcgctggag gccgcccccg gccacgcgcg
      781 gattcggatc gctcatggcg gccacgacgc gggccacgcc cgacgcgagc acggcatcga
      841 cgcacggcgg cgtgcgcccg tggtgcacgc agggttcgag cgtcacgtac agcgtgccgc
      901 caccggcgcg atcgccggcg gcgcgcaatg caagaacctc cgcgtgcggt tcgccggcgc
      961 ggcggtgcca gccctcgccc accacggctc cgctcgcatc gatgacgacg cagccgaccg
     1021 ccggattggg tgccgcggaa cagcgcccga gcgacgcgag ctgcagcgcg cgcgccatgt
     1081 gctgatggtc tacggcgctg tagtccggca tcacttcggg tcgccggcgt ccggatccgc
     1141 gccggaccac aagccgagct gcaggtcctc gacggagcgc ggccggcggt gacgcaggcg
     1201 ctcgatttcc tcgcggaagg cgtccacgtc ctggaagctg cggtacaccg acgcgaagcg
     1261 cacgtacgcg acctcgtcga gctggtgcag ttcgtccatc accagttcgc cgatcaggcg
     1321 cgccgggact tcgcgctcgc ccagcgtgcg caggcgatgg cagacgtggt tgacggccgc
     1381 gtcgatcgcc tcgctcgcga ccgggcgctt ctcgagcgcc ttgagcatgc cggagcgcag
     1441 cttggattcg tcgaacggtt cgcgggtcgc gtcgctcttg atgacgcgcg gcatgacgag
     1501 ttcggccgac tcgaaggtcg tgaagcgctc accgcagcgc aggcactcgc ggcggcgccg
     1561 cacctgctgc ccctcgccgg cgagacgcga gtcgatgacc ttggtctctt cgtgcgcgca
     1621 gaacggacag tacatgtgcc ggttccgatg cttgcgccgt ggtgggcctg ctcagccggc
     1681 cggcgcgcgg ctcagctgcc gtacaccggg aagcggcggc acagttcgcg ggtcgcctcg
     1741 cgcgtgcggg tgacgactgc ttcgtcgttg acgtcgacga gcacgtcggc gatccagtgc
     1801 gccagctggc tgacctcggc tgcgccgaac ccgcgggtgg tcacggcggg cgtgccgatg
     1861 cgcaggccgc tggtgacgaa cggcggacgc gggtcgttgg gcacggcgtt cttgttcacc
     1921 gtgatgtgcg cccggccgag cgccgcgtcc gcatccttgc cggtgtatgt cttgtcgatc
     1981 aggtcgagca ggaacaggtg attgtccgtc ccgccggaaa cgatgcggtg cccccgctcc
     2041 tgcagcacgc gacacatttc ccgcgcgttg gcgacgacct gcttctggta gtcgacgaac
     2101 tcgggctgca tggcctcgag gaaggcgacg gccttggcgg cgatgaca
//
LOCUS       DBNHBY010000711         1642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_797690, whole genome shotgun sequence.
ACCESSION   DBNHBY010000711 DBNHBY010000000
VERSION     DBNHBY010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_797690"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16HB_16850"
     CDS             complement(<1..206)
                     /locus_tag="AC16HB_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5608512.1"
                     /translation="MKTQYYTATSLDGFIATEDHSLEWLFPLGNVNDTSYPRFILDVG
                     ALAMGASTYEWMLRHVVAPAGGAAA"
     gene            complement(307..729)
                     /locus_tag="AC16HB_16855"
     CDS             complement(307..729)
                     /locus_tag="AC16HB_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608513.1"
                     /translation="MKPRIALITLGVDDLEKAVRFYRDGLGLQTPGIVGAEFAHGAVA
                     FFELQPGLRLALWPRASLAHDAGLPSSQPGATDFCLAHNVASKAEVDAVMAQARAAGA
                     VIVKSAQDTFWGGYAGYFQDPDMHLWEVAWNPQMLPAE"
     gene            complement(805..1194)
                     /locus_tag="AC16HB_16860"
     CDS             complement(805..1194)
                     /locus_tag="AC16HB_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608514.1"
                     /translation="MPATAFNHFNLRAGQPLLDKLRDFYVEVVGMKVGWRPPFNFHGY
                     WLYLGDTAVLHLVELPARSGAAEEPTGAFDHVAFSCTDLAEFEQRLEDHRIPYRRAAV
                     PGTSQQQLFVKDPAGNGVEFNFERGDA"
BASE COUNT          290 a          527 c          536 g          289 t
ORIGIN      
        1 gctgcagcgc cgccggccgg cgcaacgacg tggcgcagca tccactcgta ggtggacgcg
       61 cccatggcga gtgcgccgac atccaggatg aacctggggt agctcgtgtc gttcacgttc
      121 ccgagcggaa acaaccactc gagggagtgg tcctccgtcg cgatgaaccc gtcgaggctc
      181 gtggccgtgt agtactgggt cttcatcgtc gccaggtgcg ccgcgcggaa agatttgacg
      241 cctggcgcct gagtcgaggt cgcggctgca ttggccgaac gtcggccatc gatgcgtcgt
      301 tgtgcctcac tccgccggaa gcatttgcgg attccacgcc acctcccaca ggtgcatgtc
      361 cggatcctgg aagtagccag catagccacc ccagaaggtg tcctgtgccg acttgacgat
      421 gacggctccg gcggccctgg cctgcgccat gactgcatca acctcggctt tcgaggccac
      481 gttgtgcgcc aggcagaaat cggttgcgcc gggctggctc gatggcagcc cggcatcgtg
      541 agcaaggctg gcgcgcggcc aaagggccaa ccgcagaccg ggctgcagtt cgaagaaggc
      601 cactgcgccg tgcgcgaact ctgcgccgac aatgcccggt gtctgcagcc ccagcccgtc
      661 tcggtagaag cgaaccgcct tctccagatc atcgaccccg agggttatga gagcgatccg
      721 gggcttcact tgaccgcacc gagcgaggca tcagttcgac gctgaccggc ccgcgcgccg
      781 tggtgtgtcg ggcccacaat cgtgtcaggc gtcgccgcgc tcgaagttga actcgacgcc
      841 atttcctgcc ggatccttca cgaagagctg ctgctgggaa gttccgggta cggcggcgcg
      901 tcgatacgga atgcggtggt cttccaggcg ctgctcgaat tccgcgaggt cggtgcagga
      961 gaacgcgacg tgatcgaagg cgcccgtggg ctcctcggcc gcgccggacc gtgcaggcag
     1021 ttcgaccaga tgcaggaccg cagtgtcgcc aaggtacaac cagtacccgt gaaagttgaa
     1081 cggtggccgc cagccgactt tcatgccgac gacttcgacg taaaagtccc gcagcttgtc
     1141 gagcaagggt tgcccggcgc gcaggttgaa atgattgaag gcggtggcag gcatggccgt
     1201 gttcacctcc ccggggcgtg cggcgcgggg cgattgcccg gagccgactt ggacagcgcc
     1261 cgtgcatcct cctgactgcg cgcggcttcc tcgcgagtcc agcactgctg cgaagaaatc
     1321 ttcgatccgg tcggggcatc cttgacgcac acgacctcca gatcgcgagg tctcgttgca
     1381 tccgcgtcca ctgcgcatgc acccaatggc aacaagatga cgccgaggat caggattcgc
     1441 attgaatctc cacgccgcgg cctgcgggct gaccgaagcg gggcagttta cgacctgtta
     1501 acacttggct cgcagatgcg gcgcgcgcag tcactgacgg gcttggagca tggccgccat
     1561 gcgttgatgg acgaggttga tcgccttcag tggccgaagc atcaccttga actcaacgat
     1621 gcgcccggcg tcattccact tg
//
LOCUS       DBNHBY010000712         4020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_932606, whole genome shotgun sequence.
ACCESSION   DBNHBY010000712 DBNHBY010000000
VERSION     DBNHBY010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_932606"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1270
                     /gene="hemL"
                     /locus_tag="AC16HB_16865"
     CDS             2..1270
                     /gene="hemL"
                     /locus_tag="AC16HB_16865"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR5608515.1"
                     /translation="MFERAQRSIPGGVNSPVRAFRSVGGTPRFVARAEGPYFWDADGK
                     RYIDYIGSWGPMIVGHTHPEVVAAMQLAIARGISYGAPTEAEVEMAEELLKLVPSMQQ
                     VRLVSSGTEAAMSAIRLARGAAGDGKGRRKIVKFEGCYHGHADSLLVKAGSGALTFGN
                     PSSAGVPAEITQHTIVLDYNDVQQLEDAFVRHGDDIAGVIVEPVAGNMNLVRATPEFL
                     QSMRALCAKHGALLIFDEVMSGFRVALGGAQALYRIEPDLTVLGKVIGGGMPVAAFGG
                     KREVMKHLAPLGAVYQAGTLSGNPVAVACGRATLKLIQQPGFYERLGGQTTKLVRGLV
                     EAARAAGARFCADAVGGMFGLYFADAVPSSYAQVMACDKSRFNAFFHAMLDRGVYLAP
                     SAYEAGFVSAAHDDAAIDATVAAARAAFSA"
     gene            1306..3426
                     /locus_tag="AC16HB_16870"
     CDS             1306..3426
                     /locus_tag="AC16HB_16870"
                     /EC_number="1.8.-.-"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HIR5608516.1"
                     /translation="MSFGKFLVAPMLALAFMAAAHGQPVRADNAEVELVAATDAVVPG
                     QPLPVGLRIKHDPEWHTYWQVPGDSGLPTRIKWSLPPGWTAGPIEWPVPKRLPVGPLM
                     NFGYDGEVLLLTELTPPADLKPGGQVEFAARADFLICRDVCIPSGADLKLSLPARDAA
                     NPSRRAAQFAAMRALVPRRISLQDATAAIEDDRIRLTFRAPAALTQIEFFPLEEARIE
                     PAAPQMVQVDGDRVALHLTAAKPIADGFAQLKGVLVAGGGPARADQGGWAGEIEVTLA
                     PGKVAPTAAPRLQAATTPITLWFALLGALVGGLILNLMPCVFPVLSLKLLSLLQHQRE
                     EDAARVAHTSLRTHGLVFATGVVASFVLLAAAMLALRAGGAQLGWGFQLQTPWVIAGL
                     TALFFVIGLNLMGTFEFNVGTRLANAAPAQRLRPDRISGSFGTGVLAVVVAAPCTAPF
                     MGAALGYAITQPAAQALLVFAALGAGMALPYLLLTFFPVLLKRLPRPGSWMERFRQVM
                     AFPMFATCVWLLWVLAQQVNVNALALTLGALVLIALAVWAFGIAQRGATRWRWVALGA
                     AGVAFYAVAAATGPDSMRTGSAAVADAKSLWQPWSPAEQGRQLAAGRPVFVDFTAAWC
                     VTCQANKQLVLNSRAIVDAMADKGVSMLRADWTNRDDAITRELARFERNGVPLYVLYD
                     RSGKAQLLPELLTERVVLDALRQL"
     gene            3449..>4020
                     /locus_tag="AC16HB_16875"
     CDS             3449..>4020
                     /locus_tag="AC16HB_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5608517.1"
                     /translation="MLRRAILGVAAMSFALIAQAVALPTQDAPAFALTDVGGKRIELA
                     EFKGRYVVLEWNNPSCPFVQKHYASGNMQALQKRFTTDGVVWLTINSTAAGHPEHLRP
                     EQQAAWLKQQNAAPTTALLDADGTVGRAYGARTTPHMFVIDPAGKVRYAGAIDDRRST
                     NPADVRTSNNYVAQALGELRSGKPVTVAATP"
BASE COUNT          580 a         1290 c         1472 g          678 t
ORIGIN      
        1 gctgtttgag cgcgcgcagc ggagcattcc cggcggcgtc aactcgccgg tgcgcgcatt
       61 ccgttcggtc ggaggcaccc cgcgcttcgt cgcgcgcgcg gaggggccgt acttctggga
      121 cgccgacggc aagcgttaca tcgactacat cggctcgtgg ggaccgatga tcgtcggcca
      181 cacgcacccg gaggtggtcg cggcaatgca gttggcgatc gcgcgcggca tcagctacgg
      241 cgcgccgacc gaggccgagg tggagatggc cgaggaactg ctgaaactgg tgccgtcgat
      301 gcagcaggtg cggctggttt cctccggcac cgaggcggcg atgagtgcga ttcggctggc
      361 ccgcggcgcg gcaggggacg gcaagggccg gcgcaagatc gtcaagttcg aaggctgcta
      421 tcacggccat gccgacagcc tgctggtcaa ggccggctcg ggcgcgctga cgttcggcaa
      481 cccgagttcg gccggcgtgc cggcggagat cacacagcac acgatcgtgc tcgactacaa
      541 cgacgtgcag cagctcgaag atgccttcgt gcggcatggc gacgacatcg ctggcgtgat
      601 cgtcgagccg gtcgcgggca acatgaacct ggtgcgcgcg acgccggagt ttctgcagtc
      661 gatgcgcgcg ctgtgtgcga agcacggcgc gctgctgatc ttcgatgaag tgatgagcgg
      721 gttccgcgtg gcgctcggcg gcgcgcaggc gttgtacagg atcgagccgg acctgacggt
      781 gctcggcaag gtgatcggcg gcggtatgcc ggtggccgcc ttcggtggca agcgcgaggt
      841 gatgaagcac ctggcgccgc tcggtgcggt gtatcaggcg ggcacgctgt cgggcaatcc
      901 ggttgcggtt gcctgcgggc gggcgacgct gaagctcatt caacagcccg gcttctacga
      961 acggctcggc ggacagacga cgaagttggt gcggggcctt gtggaggcgg cgcgtgcggc
     1021 cggcgcgcgg ttttgcgccg atgcggtggg tggcatgttc ggcctgtact tcgccgacgc
     1081 ggtgccctcg agctatgcgc aggtgatggc ctgcgacaag tcgcgtttca acgccttctt
     1141 tcacgcgatg ctcgatcgcg gcgtgtatct ggcgccctcg gcctacgagg ccgggttcgt
     1201 ttcggccgcc cacgacgatg cggcgatcga cgcgacggtc gccgcggcac gcgccgcctt
     1261 ttcggcctga cggaacttgc accgctcggc agggacgaag ccttgatgag tttcggcaaa
     1321 ttcctggttg cgccgatgct ggcgctggcg ttcatggccg ccgcgcatgg gcagccggtg
     1381 cgcgccgaca acgccgaagt cgaactggtc gctgcgactg acgctgtcgt ccctggccag
     1441 ccgctgccgg tcggactgcg gatcaagcac gatcccgagt ggcacaccta ttggcaggtg
     1501 ccgggcgact ccggactgcc gacgcggatc aagtggtcgc tgccgccggg atggaccgct
     1561 gggcccatcg aatggccggt accgaagcgc ctgccggtcg gaccgctgat gaacttcggg
     1621 tacgacggcg aggtgttgct gctcacggag ctgacgccgc ccgccgatct gaagccgggt
     1681 ggccaggtcg agttcgccgc tcgcgccgat tttctgatct gccgcgacgt ctgcattccg
     1741 agcggcgccg acctcaagct gagcctgccg gcacgcgacg ccgcgaatcc gtcgcgtcgg
     1801 gcggcccaat tcgccgcgat gcgcgcgctc gtgccccggc gcatcagcct gcaggacgcg
     1861 accgcggcaa tcgaggacga ccggatacgg ttgactttcc gcgctccggc ggcgctgacg
     1921 cagatcgaat tctttccgct cgaggaagcg cgcatcgaac ccgcggcgcc gcagatggtg
     1981 caagtcgacg gcgaccgggt tgcgctgcat ctgaccgcgg caaagccgat cgccgacggc
     2041 tttgcgcaat tgaagggcgt gctcgtcgct ggcggcgggc cggcgcgcgc ggatcaaggc
     2101 ggctgggcgg gcgagatcga ggtaacgctg gcgccgggca aggtcgcgcc caccgcggcg
     2161 ccgcgcctgc aggcggcgac aacgccgatc acgctgtggt tcgcgttgct cggcgcgctc
     2221 gtcggcgggt tgatcctgaa cctgatgccg tgcgtgttcc cggtgttgtc gctgaagctg
     2281 ctgtcgctgc tgcagcacca gcgcgaagag gatgcggcgc gcgtcgcgca cacgtcgctg
     2341 cgcacgcacg gcctcgtgtt cgcgaccggg gtcgtggcga gcttcgtgct gctggcggcg
     2401 gcgatgctcg cgctgcgcgc cggcggcgcg caactgggct ggggtttcca gttacagacg
     2461 ccgtgggtga tcgccgggct gacggcgctg ttcttcgtga tcggactgaa cctgatgggc
     2521 accttcgaat tcaatgtcgg cacgcggctc gcgaacgcgg cgccggcgca gcggctgcgg
     2581 cccgatcgca tctccggcag cttcggcacg ggcgtgctgg cggtggtcgt cgcggcgccc
     2641 tgcaccgccc cgttcatggg ggcggcgctc ggctatgcga tcacgcagcc ggcggcgcag
     2701 gcgctgctcg tgttcgccgc gctaggtgcc ggcatggcct tgccctacct gctgctgact
     2761 ttcttccccg tgttgctgaa gcggctgccg cgacccgggt catggatgga gcgttttcgc
     2821 caggtgatgg cgtttccgat gtttgccacc tgcgtgtggc tgttgtgggt gctcgcccag
     2881 caggtgaacg tgaatgcgct ggcgctgacg ctgggcgcgc tggtgctgat tgcgctcgcc
     2941 gtgtgggcgt tcggcatcgc gcagcgcggg gcgacgcgct ggcgctgggt ggcgctcggc
     3001 gctgccggtg tcgccttcta cgccgtggcc gcggcgaccg gccccgattc gatgcgcacc
     3061 ggttcggccg ccgtcgccga cgcgaagagc ctctggcaac cttggagtcc ggccgagcag
     3121 ggcaggcagc tcgcggccgg cagaccggtg ttcgtcgatt tcactgctgc atggtgcgtc
     3181 acgtgtcagg ccaacaagca gctggtgctc aattcgcgtg cgatcgtcga cgcgatggcg
     3241 gacaagggcg tgtccatgct gcgcgccgac tggaccaacc gcgacgacgc aatcacgcgg
     3301 gagctggccc gcttcgagcg caacggtgtg ccgctgtacg tgctgtacga ccgcagcggc
     3361 aaggcgcaac ttctgcccga gttgctgacc gagcgcgtcg tgctggatgc cttgcgccag
     3421 ttgtgatcat gcccctcggg agatcgtcat gctgagaaga gccattcttg gcgtcgcggc
     3481 aatgtcgttt gcgctgatcg cgcaagctgt cgcattgccc acgcaggatg cgcccgcgtt
     3541 cgcgctgacc gatgttggcg gcaagcgcat cgaactggcc gagttcaagg gcagatatgt
     3601 cgtgctcgaa tggaacaatc catcgtgccc gttcgtgcag aagcactatg cgagcggcaa
     3661 catgcaggcg ctgcagaagc gcttcaccac ggacggcgtc gtgtggctga cgatcaattc
     3721 gacggcggcc gggcatccgg agcatctgcg gcccgagcag caggccgcgt ggctgaagca
     3781 gcagaatgcg gcgccgacca ccgcgctgct cgacgccgac ggcacggtgg gccgtgccta
     3841 cggcgcacgg acaacgccac acatgttcgt gatcgacccg gcgggcaagg tgcgatacgc
     3901 gggcgcgatc gacgacaggc gcagcacgaa cccggccgac gtgaggacct cgaacaacta
     3961 cgtcgcgcag gcgctcggcg aactgcgcag cggcaagccg gtcacggtgg ccgcgacgcc
//
LOCUS       DBNHBY010000713         2557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1879891, whole genome shotgun sequence.
ACCESSION   DBNHBY010000713 DBNHBY010000000
VERSION     DBNHBY010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1879891"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="AC16HB_16880"
     CDS             <1..1746
                     /locus_tag="AC16HB_16880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5608518.1"
                     /translation="NIAHIYRLREPGGALEKLLDFDDRVEQAAFEPQHGRYIVYTRDA
                     GGNEATRVYRFDPDTRASTLLSEPDMASGFAWNRGGDRLLINSVPLDRTAEGGRRERV
                     VTTLTLVDPTRPESRRTLAELPGVGWGDFRFAPDDKSLALIHYRSATDTAVWRIDVGS
                     GRREQLLPAKAETRASYFGFEFSADGKRLYLATNERSEFLQLAEYDFERKALRILSGH
                     VPWDVDTFRLSDDGARLIAVFNANGRDEAVLFDPATGKEIARPQLPAGQLRGVEFHRA
                     DAREIAFSADSPQSPGTVWSYDVDRDRLTRWTTPLTEGVDPSSFADAQIVAWKSFDGR
                     TISGILYLPPAKFTGPRPVIMLIHGGPEAQAKVGFNNRFNYLINELGIALLEPNVRGS
                     AGFGKTFVDLDNGTKREDSVKDIGAALDWIGTQPRLDASRVVVAGGSYGGYMVHASLV
                     HYSERLRGGIADVGIANFVSFLQNTESYRRDLRRAEYGDERDPAMRAFLERISPVNNA
                     DRIRAPLFVIHGRNDPRVPYTEAEQIVAAVRANKVPVWYLLADNEGHGFARKDNADFR
                     FYAVVKFLEQTLLAK"
     gene            1743..2237
                     /locus_tag="AC16HB_16885"
     CDS             1743..2237
                     /locus_tag="AC16HB_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5608519.1"
                     /translation="MKTLRDLHAPPAVVGRVEAIVVRRASREPARAIDATVAIAGVGL
                     ADDRLGKRGEPELTTRQVTLIQHEHLPVIAALARVEVVDPVGLRRNLVISGINLAALK
                     NARLRVGAAVLEIVGPCQPCSRMEEAIGPGGYAAMRGHGGMTARVLTGGAIRVGDTVR
                     AASA"
     gene            complement(2229..>2557)
                     /locus_tag="AC16HB_16890"
     CDS             complement(2229..>2557)
                     /locus_tag="AC16HB_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5608520.1"
                     /translation="PRIFELYLNTVDWGPGICGARAAARTYFGKRPAQLTALEAAWLA
                     GILRNPHAAYRQQFVARRPDAQRATAVLMQMRTPARRMRERAAREVLVFQPPATAPAA
                     TRFASR"
BASE COUNT          400 a          887 c          910 g          360 t
ORIGIN      
        1 aacatcgcgc acatctatcg gctgcgcgag ccgggcggcg cgctggagaa gctgctcgac
       61 ttcgacgacc gtgtcgagca ggccgcgttc gagccgcagc acgggcgcta catcgtctac
      121 acgcgcgacg ccggcggcaa cgaggccacc cgcgtctacc gcttcgatcc cgacacgcgc
      181 gccagtacgc tgctgtccga gcccgacatg gcgagcggct tcgcatggaa tcgcggcggc
      241 gaccggctgt tgatcaattc ggtgccgctg gaccgcacgg cggagggcgg ccggcgcgag
      301 cgcgtggtca cgaccctgac gctggtcgac ccgacccggc ccgagtccag acgcaccctc
      361 gcagaactgc ccggcgtcgg ctggggcgac ttccgcttcg cgcccgacga caagtcgctg
      421 gcgctgatcc actaccgcag cgccaccgac acggcggtct ggcgcatcga cgtcggaagc
      481 gggcggcgcg agcaattgct gccggcgaag gcagagacgc gggcgagcta tttcggcttc
      541 gagttcagcg cggacggcaa gcggctgtat ctcgccacca acgagcgcag cgaattcctg
      601 cagctcgccg agtacgactt cgaacgcaag gcgctgcgca tcctgtcggg ccacgttccg
      661 tgggacgtcg acacgttcag gctgtcggac gatggcgcac gcctgatcgc cgtattcaac
      721 gccaacggcc gcgacgaggc ggtgctgttc gatcccgcca ccggcaagga gatcgcgcgg
      781 ccgcaactgc ccgcgggtca gttgcgaggg gtcgagtttc atcgggccga tgcgcgcgag
      841 attgcgttct cggccgattc gccgcagagc ccggggaccg tgtggtcgta cgacgtcgac
      901 cgcgacaggc tcacgcgctg gaccacgcct ctgaccgagg gcgtcgaccc gtcgtcgttc
      961 gccgacgcgc agatcgtcgc gtggaagagc ttcgacggcc gcacgatcag cggcatcctg
     1021 tacctgccgc cggcgaagtt cacgggcccg cgcccggtga tcatgctgat ccacggcgga
     1081 ccggaagcgc aggccaaggt cggattcaac aaccgattca actacctcat caacgagctc
     1141 ggcatcgcgc tgctggagcc gaacgtgcgc ggctcggcgg gtttcggcaa gacgttcgtg
     1201 gacctcgaca acggcacgaa gcgcgaggac tcggtcaagg acatcggcgc ggcgctggac
     1261 tggatcggca cgcagccgcg gctggacgcg agccgcgtcg tcgtggccgg cggcagctac
     1321 ggcggctaca tggtgcatgc gagcctcgtg cactatagcg agcggctgcg cggcggcatt
     1381 gccgatgtcg gcattgccaa cttcgtcagc ttcctgcaga acaccgaaag ctaccggcgc
     1441 gacctgcgcc gcgccgagta cggcgacgag cgcgaccccg cgatgcgcgc gttcctggaa
     1501 cggatctcgc cggtcaacaa cgccgaccgg atccgcgcgc cgctgttcgt gatccacggc
     1561 cggaacgacc cgcgcgtgcc ctacaccgag gccgagcaga tcgtggctgc cgtgcgcgcg
     1621 aacaaggtgc cggtgtggta tctgctcgcc gacaacgagg ggcatggctt cgcgcgcaag
     1681 gacaacgcgg actttcgctt ctacgcggtc gtgaagttcc tcgaacagac gctgctcgcg
     1741 aagtgaagac gctgcgcgac ctccatgcgc cgccggccgt cgtcgggcgc gtggaggcga
     1801 tcgtcgtgcg ccgcgcgtcg cgcgagccgg cgcgcgcgat cgacgcgacg gtcgcgatcg
     1861 ccggggtcgg gctcgccgac gaccggctcg ggaaacgtgg cgagcccgag ctgaccacgc
     1921 gccaggtgac gctgattcag cacgagcacc tgccggtgat cgccgcgctc gcgcgcgtcg
     1981 aagtggtcga tcccgtgggg ctgcggcgca acctcgtcat ctccggcatc aatctcgcag
     2041 cgctgaagaa cgcgcggctg cgcgtcggcg cggcggtgct ggagatcgtc gggccctgcc
     2101 agccctgctc gcggatggaa gaggcgatcg gccccggcgg ttacgcggcg atgcggggtc
     2161 acggcggcat gacggcgcgc gttctcaccg gcggggcaat ccgcgttggc gacacggtcc
     2221 gtgcggcttc agcgtgacgc gaagcgcgtc gcggcgggcg ccgtggccgg cggctggaaa
     2281 accagcacct cgcgcgcggc gcgctcgcgc atgcggcgcg ccggcgtgcg catctgcatc
     2341 agcaccgcag tcgcgcgctg cgcgtccggc ctacgcgcga cgaactgctg ccggtacgcc
     2401 gcgtgcggat tgcgcaggat gccggccagc cacgccgcct ccagcgcggt caactgcgcc
     2461 ggccgcttgc cgaagtaagt gcgcgcggcg gcgcgggcac cgcagatgcc gggaccccag
     2521 tcgaccgtgt tcaggtacag ctcgaagatg cgcggct
//
LOCUS       DBNHBY010000714         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_256487, whole genome shotgun sequence.
ACCESSION   DBNHBY010000714 DBNHBY010000000
VERSION     DBNHBY010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_256487"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="AC16HB_16895"
     CDS             complement(<1..210)
                     /locus_tag="AC16HB_16895"
                     /inference="COORDINATES: protein motif:HMM:NF042569.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HIR5608521.1"
                     /translation="MNIPVAAATTRDHLVQECAQTLVDAFAGYNAEFRAITQRARQRF
                     EDRDWRGSQKDAVERIELYEKYVDGT"
     gene            complement(257..1558)
                     /gene="icd"
                     /locus_tag="AC16HB_16900"
     CDS             complement(257..1558)
                     /gene="icd"
                     /locus_tag="AC16HB_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR5608522.1"
                     /translation="MSTALKLVVPPNGQKITNQDGKLVVPDQPIVPFIEGDGTGPDIW
                     RASVRVLDAAVQKAYGGKRKIHWMEVYAGQKANDQFNTWLPDETVDACREYLVSIKGP
                     LTTPIGGGIRSLNVALRQMLDLYVCLRPVRWFRGVPSPVKAPEKVDMVIFRENTEDIY
                     AGIEFEAGSPDNDKFKKLFKEAFPKMYGKMRFPETSGIGIKPVSKDGTERLFRAAVEY
                     AIVNKRKSVTIVHKGNIMKFTEGAFRNWSYALAEREFPQQTYTWDQWERTKAAKGEKA
                     ANAEQDAARAAGRILIKDAIADITLQQVLTRPDEFDVIATMNLNGDYLSDALAAQVGG
                     IGIAPGGNINYMTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYMGWFEAADA
                     IISAMDKTIGQKTVTYDFARLMAGATEVKCSEFGDALIRNL"
     gene            complement(1645..>2125)
                     /locus_tag="AC16HB_16905"
     CDS             complement(1645..>2125)
                     /locus_tag="AC16HB_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608523.1"
                     /translation="ARLTDGRWSTPVAVHHDGWRIAACPVNGPAIAARDQLVAVAWFT
                     AAGGTPRVRLAYSSDGGRSFAAPIDVASGAVAGRVDLVLLDSGHSVVSWLAEPGAIRA
                     RVCGTAGCEPDVLTVADTDVARGSGFPRMAAVGHGLVFAWTAHAAVPELRTAYVALQ"
BASE COUNT          300 a          723 c          730 g          372 t
ORIGIN      
        1 ggtcccgtcg acgtacttct cgtacagctc gatgcgctcg acggcatcct tctggctgcc
       61 gcgccagtcg cggtcctcga agcgctgccg cgcgcgctgc gtgatggcgc ggaactcggc
      121 gttgtagccg gcgaacgcgt cgaccagcgt ctgcgcgcat tcctgcacga gatggtcgcg
      181 cgtcgtcgcc gcggccaccg ggatgttcat cgcgcgcagc gctccttgcg cggcgaccgt
      241 cgcggacccg ccaccctcag aggttgcgga tcagcgcgtc gccgaattcg ctgcacttga
      301 cctcggtggc gcctgccatg agccgcgcga agtcgtaggt gaccgtcttc tggccgatgg
      361 tcttgtccat tgccgagatg atcgcgtcgg ccgcctcgaa ccagcccatg tagcgcagca
      421 tcatctcgcc ggacaggatc accgagcccg ggttgacctt gtccaggttg gcgtacttcg
      481 gcgctgtgcc gtgcgtcgcc tcgaacaccg cgtgacccgt catgtagttg atgttgccgc
      541 ccggcgcgat gccgatgccg ccgacctggg ccgccagggc gtcggagagg tagtcgccgt
      601 tgaggttcat cgtggcgatg acgtcgaatt cgtcggggcg cgtcagcacc tgctgcagcg
      661 tgatgtccgc gatcgcgtcc ttgatcagga tgcggcccgc ggcgcgcgcg gcgtcctgct
      721 cggcattggc ggccttttcg cccttggcgg ccttggtgcg ctcccactgg tcccacgtgt
      781 aggtctgctg cgggaactcg cgctcggcga gcgcgtacga ccagttgcgg aacgcgccct
      841 ccgtgaactt catgatgttg cccttgtgca cgatcgtcac gctcttgcgc ttgttgacga
      901 tcgcgtactc gacggccgcc cggaacaggc gctcggtgcc gtccttcgac accggcttga
      961 tgccgatgcc ggaggtctcc gggaagcgca tcttcccgta catcttcggg aaggcttcct
     1021 tgaacagctt cttgaacttg tcgttgtcgg ggctgcccgc ctcgaactcg atgccggcgt
     1081 agatgtcctc ggtgttctcg cggaagatca ccatgtcgac cttctccgga gccttcaccg
     1141 gggagggcac gccgcggaac cagcgcaccg ggcgcaggca gacgtacagg tcgagcatct
     1201 ggcgcagcgc gacgttcagc gagcggatgc cgccgccgat cggcgtggtc agcggcccct
     1261 tgatcgacac caggtattcg cggcaggcgt cgaccgtctc gtcgggcagc cacgtgttga
     1321 actggtcgtt cgccttctgt ccggcgtaga cctccatcca gtggatcttg cgcttgccgc
     1381 cgtaggcctt ctgcacggcc gcgtcgagca cccgcaccga agcgcgccag atgtccggcc
     1441 ccgtgccgtc accctcgatg aaggggacga tcggctggtc cggcacgacg agcttgccgt
     1501 cctggttggt gatcttctgg ccgttgggcg gcacgacgag cttgagcgcg gtagacatcg
     1561 acctggctcc gatttgttct ggattggcgc gaaaaacgcg taatccggaa tgctagcaca
     1621 gcggttccgg agcgggagcg ggcctcattg cagcgcgacg tacgccgtgc gcagctcggg
     1681 aacggcggcg tgcgccgtcc acgcgaatac caggccgtgg ccgacagccg ccatgcgcgg
     1741 aaagccggag ccgcgcgcga cgtcggtgtc ggccaccgtc agcacgtccg gctcgcagcc
     1801 ggcggtcccg cagacgcgcg cacggatggc gccgggttcg gcgagccagc tcacgaccga
     1861 gtgaccggag tccagcagga cgaggtcgac gcggccggcg actgctccgc tggcgacgtc
     1921 gatcggcgcc gcgaacgaac gcccgccgtc gctcgaatac gcgaggcgca cgcgcggcgt
     1981 accgccggcg gccgtgaacc acgccacggc cacgagctgg tcgcgggccg caatggccgg
     2041 gccgttgacg gggcaggctg cgatccgcca gccgtcgtga tgcacggcga ccggggtgga
     2101 ccagcgacca tcggtgaggc gcgcc
//
LOCUS       DBNHBY010000715         1916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1137521, whole genome shotgun sequence.
ACCESSION   DBNHBY010000715 DBNHBY010000000
VERSION     DBNHBY010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1137521"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16HB_16910"
     CDS             complement(<1..356)
                     /locus_tag="AC16HB_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5608524.1"
                     /translation="MRKTAMILCLSALIPSVSDADALAGKRKAEKCVTCHVATEGTQF
                     LPVLDGQTRDYFIAQIRAFQARRRTDWAMITNTSSLTESDVRDLADFFEARKPPAYPI
                     RDAKRAERGREKLQAL"
     gene            complement(447..1292)
                     /locus_tag="AC16HB_16915"
     CDS             complement(447..1292)
                     /locus_tag="AC16HB_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HIR5608525.1"
                     /translation="MADSTHWTCPTCKVTVATPFCARCGEEPIAPGDLTLRALVGKAL
                     HALTSIDARAARSARKLLLHPGELTLAWTGGVRKPFVAPFQLFLIANVLFFALQWATN
                     ENVFSTSLDSHLHHQDWSELAQSVLSRRLEATHTTLAQYEPTFDRAVVLNAKSLILLM
                     TLPFALLLPLAFARERRPFMTHVVFSLHLYTFLLLLFCLALLAARLSALLGFGGLDTP
                     MVDNVLSVANLTVCALYVYLAIGPVYGATGTRRFAKAIVLALAAAAIVVGYRFALFFI
                     TLYGT"
     gene            complement(1697..>1916)
                     /locus_tag="AC16HB_16920"
     CDS             complement(1697..>1916)
                     /locus_tag="AC16HB_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5608526.1"
                     /translation="AKAAELDKTIRKLSAMRDGLQHAAVCPAPSHMECPTLRRLLREA
                     ASGAMGERSSKRASALRRPDVARRGQRR"
BASE COUNT          379 a          601 c          614 g          322 t
ORIGIN      
        1 ctcaatgcct gcagcttctc cctcccgcgt tctgcgcgct tggcatcgcg aatcggatag
       61 gcgggcggct tccgggcctc gaagaagtcg gcaagatcgc gaacgtcgct ctccgtcaag
      121 ctcgaggtat tcgtgatcat cgcccagtcc gtgcggcgcc tcgcctggaa cgccctgatc
      181 tgcgcaatga aatagtctct ggtctggccg tccaagacag gcaggaactg tgttccttcc
      241 gttgcgacat ggcacgtcac acacttctct gccttccgtt tcccggcgag cgcatcggca
      301 tcggacactg acgggataag tgccgaaagg cagagaatca tcgcggtctt tctcatgacg
      361 caatgctcca aaatgtgcct gacgttcgag atcagccgcc ctgcgcaggc tgcagcaagg
      421 catcaggaat cgcttcgacg gcccattcag gttccgtaca gggtgatgaa gaaaagcgcg
      481 aagcgatagc caaccacgat ggctgctgcc gccagcgcca gcacgatcgc cttggcaaac
      541 cggcgcgtgc cggtagcgcc gtacaccggg ccgatcgcca gatagacgta gagcgcgcac
      601 acggtgagat tcgcgacact cagcacgttg tcgaccatcg gggtgtcgag cccgccgaag
      661 ccgagcaacg cgctgagcct ggctgccagc agagccaggc agaacagcag cagcaggaac
      721 gtgtagaggt gcagcgagaa gaccacgtga gtcatgaatg gccgacgttc gcgcgcaaag
      781 gccagcggga gtagcaaagc aaacggcaac gtcatcagca ggatgagcga cttggcgttc
      841 aatacgacgg cgcggtcgaa tgtcggttcg tactgcgcga gcgtcgtatg agttgcctcc
      901 aatcgccgag acaagaccga ctgggccagt tcgctccaat cctgatggtg aaggtgcgag
      961 tcgagcgaag tgctgaagac gttctcgttg gtcgcccact gcagggcgaa gaagagaacg
     1021 ttggcgatca gaaacagctg gaacggcgcc acgaacggct tgcggacacc gcccgtccac
     1081 gccaatgtca gctctccggg atgaagcaac agctttcggg ctgatcgcgc cgcgcgcgca
     1141 tcgatgctgg tcagggcatg gagtgccttc ccgacgaggg cacgcagggt gaggtcacct
     1201 ggcgcgatcg gctcttcgcc gcaccgagca caaaatggcg ttgccacggt caccttgcag
     1261 gtcggacaag tccaatgcgt actgtcggcc atggcaacga cctcctgtgc gggaccgggt
     1321 catgacacca aaggcgccac aaccgatcga cgagtgtgca cccttcttca gcgcccctcc
     1381 attgcagtcg cggcgctcag gtgcttggtg tgacgtctga cgtttgtgct cagccgcctg
     1441 cggatgttgg cgaagccggc cgctgcagat cagcgttgga cggcacagcg caccccgtgc
     1501 acggcgcgcc cagtcgaccg cgacggatgc caagaggtcg tgggagtccc gggtcggtat
     1561 caagacccgg tcgcggaagt gggccagccc gtatccgatc gaaggaaatg cagcgaatac
     1621 gaccgagcca agcggggcga tctggcaaat cggccgacgc gtggcgacgg tggcactccc
     1681 ccaatatgtg cggcgactag cgccgttgcc cccgccgcgc aacgtcgggg cgccgaagtg
     1741 ctgacgccct cttgctgctt cgctcaccca tggctccaga tgccgcttcc cgcagcaggc
     1801 gccgaagggt cgggcactcc atgtggctcg gggcgggaca gacggcagcg tgttgcaggc
     1861 cgtcgcgcat ggcgctcagc ttgcgaatcg tcttgtccaa ctcggccgcc ttggcc
//
LOCUS       DBNHBY010000716         3274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1879991, whole genome shotgun sequence.
ACCESSION   DBNHBY010000716 DBNHBY010000000
VERSION     DBNHBY010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1879991"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16HB_16925"
     CDS             complement(<1..236)
                     /locus_tag="AC16HB_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HIR5608527.1"
                     /translation="MTSRPPLGTPLSPAAARVILLGAGELGKEVIIALQRLGVETIAA
                     DRYPDAPGHQVAHRAHVLNMADPEALRALIERERP"
     gene            complement(355..1116)
                     /locus_tag="AC16HB_16930"
     CDS             complement(355..1116)
                     /locus_tag="AC16HB_16930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5608528.1"
                     /translation="MFIDSHCHLSFPDLAGDIEGVLGRMRAAGVIGALNVSVTLEGFP
                     AVVALAEAQPDIWASIGVHPDHREGREPDVATLVRLAQHPKVVAIGETGLDYYRLGEP
                     LDWQRDRFRTHIRAAREAGLPLIVHTRAAAEDTLRIMREEHAEEAGGVMHCFTESLHV
                     AKAALELGFYISFSGIVTFKSAAELRDVASAVPLDRLLIETDAPYLAPVPHRGRSNEP
                     AFVPFVAGAIGLVKGLDRDRIGAETSAGFKRLFNV"
     gene            complement(1154..1549)
                     /locus_tag="AC16HB_16935"
     CDS             complement(1154..1549)
                     /locus_tag="AC16HB_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5608529.1"
                     /translation="MATAPGPGTLGGGAAAPGSGARPAVLSLAIKEKAALYAAYMPYL
                     RNGGIFVPTNKPYQLGDEVYLILTLMDDPTKLPIAGRVVWISPPGGGQRQPGIGVHFP
                     GDESGVAARSRIEDILGTALKSARPTHTI"
     gene            complement(1570..2652)
                     /gene="holB"
                     /locus_tag="AC16HB_16940"
     CDS             complement(1570..2652)
                     /gene="holB"
                     /locus_tag="AC16HB_16940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR5608530.1"
                     /translation="MDRDLTLPRRLPWHDATWAALQSLKQRGVHAVLLHGARGIGKKS
                     LGFDLAQAALCERPTASGRACGQCTGCVLMEAGSHPDFRFVLPDALASLRPGHALDAA
                     ADEPPPVEVEESDKPKKLSREIRIEQVRSLADLATVATHRGGGRVILLAPAEALNAPA
                     ANALLKLLEEPPDRTLFVLISDALDELPPTIRSRCVLVRVEAPSPSSALQWLAGQGID
                     DVHTALAAAGGAPLEVLEGSSGAALGAELRALLLEFLERGEALDPADIVRRLPRDIAV
                     APAVSLLQRWCWDLLGCRAAGRVRYHPGHQAGIARLARRAMPEALWSWIDALERAQAT
                     RDHPLNARLVLESLLLQYRNCMTSTR"
     gene            complement(2637..3257)
                     /gene="tmk"
                     /locus_tag="AC16HB_16945"
     CDS             complement(2637..3257)
                     /gene="tmk"
                     /locus_tag="AC16HB_16945"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5608531.1"
                     /translation="MTSRGRFITFEGIDGAGKSTHIEFVRDELQRAGRPVLVTREPGG
                     TALGERLRELVLRHPMSARTETLLLFAARSEHLEQVIRPALARGQWVLCDRFTDATYA
                     YQSGGRRLPELQIETLERWVHPDLQPDLTLLFDLEPAIAAQRLAAARAADRFESEQGD
                     FFARVRAAYLARAQRFPGRFVVLDGAATVDSIRHDIAARLKPWIAT"
BASE COUNT          548 a         1131 c         1073 g          522 t
ORIGIN      
        1 ggccgctcgc gctcgatcag ggcgcgcagc gcttcggggt ccgccatgtt gagcacgtgc
       61 gcccgatgcg caacctgatg gcctggcgcg tcgggatagc ggtccgcggc gatcgtttcg
      121 acgccgaggc gctgcagggc gatgatgact tccttgccca gttctcccgc gccgagcagt
      181 atcacgcgcg cggcggccgg ggacaggggc gtcccgagcg gcgggcgcga tgtcaaggag
      241 ctagcggctg gcaccgttac ctcggatagt taacataatg cacattatgc gcagtcaagc
      301 aaacttgaag taatttctca tgaaaacctg cggacacgca ttaattttat cgccttagac
      361 gttgaaaaga cgcttgaatc ctgcgctcgt ctcagcgccg atccggtccc gatcgaggcc
      421 tttcactagg ccgattgcgc cggccacgaa cggaacgaac gccggctcgt tggaccgccc
      481 gcgatgcggc accggcgcca gatacggcgc gtcggtttcg atcaacagac gatcaagtgg
      541 caccgccgac gccacgtccc gcagttcagc cgcgctcttg aaggtcacga tgcctgaaaa
      601 cgagatgtag aaacccagtt cgagtgccgc tttcgccacg tgcagcgact ccgtgaagca
      661 atgcatgacg ccgcccgctt cctccgcatg ctcttcacgc atgatccgca acgtgtcctc
      721 ggccgccgcc cgggtgtgga caatcaacgg caagccggcc tcgcgtgcgg cccggatatg
      781 ggtccggaat cggtcgcgtt gccagtcgag tggctcgccc agccggtagt agtcgagccc
      841 ggtttcgccg atcgcgacga cctttgggtg ctgcgcgagc cgcaccagcg tcgcgacgtc
      901 cggttcgcga ccctcgcgat ggtcgggatg cacaccgatc gaggcccaga tgtcgggctg
      961 cgcctcggcc aatgcaacga cggcagggaa accttccagc gtcacgctca cgttgagggc
     1021 gccaatgacg ccggccgctc gcatgcggcc gagtacgccc tcgatgtcgc cggcgagatc
     1081 gggaaacgac aggtgacagt gcgaatcgat gaacacgcgt tttcagcgtc gacctgcagg
     1141 cgcggaacgc gcgtcagatc gtgtgcgtcg gtcgcgcgga cttgagcgcc gtgccgagta
     1201 tgtcctcgat gcggctgcgc gcggcaacgc cgctctcatc gcccggaaaa tgcacgccga
     1261 tgccaggttg tctctgaccc ccgcccggcg ggctgatcca gacgacccgg ccggcgatcg
     1321 gcaacttggt cggatcgtcc atcagcgtca ggatcagata cacctcgtcg ccgagctgat
     1381 agggtttgtt ggtcggcacg aagatgccgc cgttcctcag gtacggcatg taggcagcgt
     1441 acagcgcggc cttttccttg atcgccagcg acagcacggc cggacgcgcg cccgagcccg
     1501 gggctgccgc gcctcctcca agtgttcccg gtccgggtgc agtcgccatg tcgttcgtcg
     1561 tcaggaagtt cagcgcgtcg aggtcatgca gttccggtac tgaagcagca gcgattccag
     1621 cacgagccga gcattcagcg ggtggtcacg ggtcgcctgc gcgcgctcga gcgcatcgat
     1681 ccagctccac agcgcctcgg gcatggctcg ccgcgccagc cgcgcgatgc ctgcttggtg
     1741 cccgggatga taccgaaccc gccccgcagc gcggcatccg agcaaatccc agcaccagcg
     1801 ctgcaacagt gagaccgcgg gcgccactgc aatgtcacgc ggcagccggc gcacgatgtc
     1861 cgcaggatcg agggcttcac cgcgctcgag gaactccagc aacagcgctc gcagttccgc
     1921 gcccagcgct gcgccagacg agccttccag cacctcgagc ggcgccccgc ctgccgcggc
     1981 gagcgcggtg tggacatcgt cgatgccctg cccggccagc cattgcaggg cgctcgacgg
     2041 agacggcgcc tcgacccgca ccagcacgca gcgcgatcgg atcgtcggcg gcaattcgtc
     2101 gagcgcatcg ctgatcagca cgaacaaagt ccgatcgggt ggctcctcca gcagtttcaa
     2161 cagcgcgttg gcggctgggg cgttcagcgc ttcggccggt gccagcagga tcacgcgccc
     2221 gccgccgcga tgtgtagcca cggtcgccaa gtccgccagg gagcgaacct gttcgatgcg
     2281 gatctcacgg ctcagtttct tcggcttgtc cgattcctcc acttcgaccg gcggcggctc
     2341 atccgcggcg gcgtcgagtg catggcctgg ccgcagggac gccagcgcat ccgggagcac
     2401 gaagcggaaa tccggatggc tgcccgcctc catcagcacg cagccggtgc attggccgca
     2461 cgcgcgaccg ctcgccgtcg gacgctcgca gagggcggcc tgtgccagat cgaagccgag
     2521 gctcttcttg ccgattccgc gcgcaccgtg cagcagcacc gcgtgcacgc cgcgctgctt
     2581 cagcgactgc agcgcggccc aggtggcatc gtgccacggc agacggcgcg gcagcgtcag
     2641 gtcgcgatcc atggtttcag gcgcgcggca atatcgtgcc ggatcgagtc gaccgttgcc
     2701 gcgccatcga gcacgacgaa gcggcccggg aaacgctgcg cacgcgccag gtatgccgca
     2761 cgcacgcgcg cgaagaagtc cccctgctcg gattcgaatc gatcggccgc gcgcgccgcc
     2821 gcgagccgct gcgccgcgat ggcgggctcc agatcgaaca gcagtgtcag gtccggctgc
     2881 aggtcggggt ggacccatcg ctccagtgtc tcgatctgca actcgggcag ccggcggccg
     2941 ccggactgat acgcataggt cgcatcggtg aatcgatcac acagcaccca ttggccacgt
     3001 gcgagcgcag gccggatcac ctgctccaga tgttcactgc gcgcggcgaa cagcagcagc
     3061 gtctcggtac gcgcgctcat cgggtgccgc agcacgagtt cgcgcaagcg ctcgccaagc
     3121 gcggtgccgc ccggctcgcg cgtgaccagc accggccgcc ccgcgcgctg cagttcatcg
     3181 cgcacgaatt cgatgtgcgt gctcttgccg gcgccgtcga tgccttcgaa cgtgatgaag
     3241 cggccccgcg aggtcatcgt ttgccgccga gctg
//
LOCUS       DBNHBY010000717         5298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_391282, whole genome shotgun sequence.
ACCESSION   DBNHBY010000717 DBNHBY010000000
VERSION     DBNHBY010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_391282"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="AC16HB_16950"
     CDS             complement(<1..968)
                     /locus_tag="AC16HB_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5608532.1"
                     /translation="MKSKAILPLLALVVACAGAAAWWYQRNDKAPANLLQLHGNVDIR
                     QVELAFNGSERIAQVLVREGDRVRKDQLLAVLDTERLKHAVAQAEAQVAAQQQVVTRL
                     VAGSRPQEIRKAQADVQAARVDLRNARSNYERDQELVAKRFISQQQADNSRAAADAAE
                     ARLNAAQETLRLVEVGSRKEDIAAAKATLAAFEAALALAQRNLGDANLRAPADGVVEN
                     RILEAGDMASPQKAVLTLALTDPLWVRTYVSETDLGKVRTGARAEIATDSYPDKRYRG
                     WIGFISPTAEFTPKSVETREVRTVLVYQVRVFACAPTDELRLGMPAT"
     gene            complement(1160..2989)
                     /locus_tag="AC16HB_16955"
     CDS             complement(1160..2989)
                     /locus_tag="AC16HB_16955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR5608533.1"
                     /translation="MDTLTAHRSDIRARIDALRAAMQEESIAAWLVPSADPHLSEYLP
                     ERWQGRQWLSGFTGSVGSLIVTADFAGLWVDSRYWVQAETQTAGTGITVMRIGGATSS
                     AHIDWLAEHVPAGGTVGVDGAVLSLAQSRALEDRLAPRRVKLRTDLDLLDRVWRDRPP
                     LPAAPVREHARAHAPVARPDKLAAVRAQMQAAGVDWHLISTLDDIAWLFNLRGADVPY
                     NPVFVAHALIGPQRATLFVGEGKVPPGLADRLEGEGVDVRPYEWAAAALANLPRNAAL
                     LIDPKRVTLGMRAQVPAHVRIVEALNPSTLAKSRKTDAEIAHVRRAMEQDGAALCEFF
                     TWLEAALAQGERITELTIDEHITAARAQRPGFVSPSFSTIAGFNANGALPHYRATAES
                     HVVISAAGSPGNGLLLIDSGAQYDYGTTDITRVVPIGTTSAEQQRDYTLVLKGMIQLS
                     MAVFPRGTRSPLLDTLARAPIWAGGVDYGHGTGHGVGYALNVHEGPHGITPHLAPEPA
                     TAMEPGMITSNEPGIYRPGRWGVRIENLVLNVPAHAGEFGEFLRFETLTLCPIDTRCI
                     ERALLRADEVAWLNAYHAEVKRRLSPFVSGDALAWLERRTQPF"
     gene            complement(3241..4167)
                     /gene="rarD"
                     /locus_tag="AC16HB_16960"
     CDS             complement(3241..4167)
                     /gene="rarD"
                     /locus_tag="AC16HB_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR5608534.1"
                     /translation="MNRGILYALGAYFVWGVVPLYFKALHGVPAPEILAHRMAWSLAV
                     CALLLAALRRWQWLRILAQRPRVLAAFVVSALLVTVNWGTYIWAIGADHVIDASLGYY
                     INPLVNVLIGAFVLQERLRRPQWTAVSVAAAGVAWLTWDAGAPPWIGLALALTWGAYG
                     LLRKTAALGAIEGLALETALLFPFALAYLLWLAAQGQDAFTPGTDATRWLLAAAGPVT
                     ATPLLLFAASARRIPFAHLGILQYISPTLQLALGVWVFHEPFAASKLAGYALIWIALA
                     IFAVDGLWQLRRPMREAGRNEKERTPGQKKAA"
     gene            4263..5195
                     /locus_tag="AC16HB_16965"
     CDS             4263..5195
                     /locus_tag="AC16HB_16965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HIR5608535.1"
                     /translation="MTDRQLEDDVARVRAFNRFYTQTIGVLDEGLLDSSFSLTEGRLL
                     WELAHRDDLTAGELARALNLDPGYVSRMLARFRGRGLVAREVSATDRRRRHLALTKRG
                     ARAFAPLDRRAHDEVAALLGRLAPGERRRLLQSLQTVESLLGARTERVAPYVLRPHRA
                     GDIGWVVARHGALYHEEYGWDLTFEALVAEIGAEFLRNFDPQREHCWIAERDGVPVGS
                     VFCVAHSRRVAKLRLLLVEPAARGSGLGAALVAECIRFARAAGYHTLTLWTNDVLVAA
                     RRIYERCGFRLVRQEKHHSFGHDLVGQYWKLPLR"
BASE COUNT          784 a         1859 c         1853 g          802 t
ORIGIN      
        1 gtggccggca tgccgagccg cagttcgtcc gtgggggcgc acgcgaagac gcgcacctgg
       61 tatacgagca ccgtgcggac ctcgcgcgtt tccacggact tgggcgtgaa ctccgccgtc
      121 ggcgagatga agccgatcca gccgcggtag cgcttgtccg gataactgtc ggtcgcgatc
      181 tcggcacgcg cgccggtgcg gaccttgccg agatcggtct cggagacata ggtgcgcacc
      241 cacagcgggt cggtcagcgc cagcgtcaag acggccttct ggggcgaggc catgtcgccc
      301 gcctcgagga tgcggttctc caccacgcca tcggccggcg cgcgcaggtt ggcgtcgccg
      361 aggttgcgct gcgcgagtgc cagcgccgcc tcgaacgcgg cgagggtcgc tttcgcggca
      421 gcgatgtcct ccttgcgcga gcccacttcc accaggcgca gcgtctcctg cgcggcgttc
      481 aggcgcgcct cggcggcgtc ggcggcggcg cgcgagttgt ccgcctgctg ctgcgagatg
      541 aaacgcttgg cgacaagctc ctggtcgcgc tcgtagttgc tgcgtgcatt gcgcagatcg
      601 acccgcgccg cttgcacgtc ggcctgcgcc ttgcggatct cctgtggccg cgagccggcg
      661 acgagtcgcg tcacgacttg ctgctgggcg gcgacctgcg cctcggcctg cgcgacggcg
      721 tgcttcagcc gttcggtgtc gagcacggcc agcaactggt ccttccgcac ccgatcgcct
      781 tctcgcacca gcacctgcgc gatgcgttca ctgccgttga aagccagctc gacctgccgg
      841 atgtcgacat tgccgtgcag ttgcaggagg ttggcggggg ccttgtcgtt gcgctgatac
      901 caccacgcgg ctgcgcccgc gcaggcgacg acaagtgcaa ggaggggcag gatcgctttt
      961 gacttcatag tggccaccgc cgcgtggcat cgtagcccag cgcgcgcgcg ctgagggcag
     1021 ctacatcgcc gctgcccgtg cgcctcccag ccccagcggg ggcggagtca tgctgcccgc
     1081 atgtgccgcg cttcgtgcac ggcgtgatcc gccgccgcag tcggtcgcgc tggcacgcga
     1141 tcggcagatg ctcgaccggt caaaacggct gcgtgcgccg ctcgagccac gccagcgcgt
     1201 ccccgctcac gaacggcgag agacggcgct tcacctcggc atggtaggca ttcagccatg
     1261 cgacctcgtc cgcgcgcagc agcgcgcgct cgatgcagcg cgtgtcgatg ggacacaacg
     1321 tcaacgtctc gaaccgcaga aactcgccga actcgcccgc atgcgcaggg acgttcagca
     1381 ccaggttctc gatgcgcacg ccccagcggc cgggacggta gatgccgggc tcgttgctgg
     1441 tgatcattcc cggctccatc gcggtcgccg gctcgggcgc gaggtgcggc gtgatgccgt
     1501 gcggtccttc atgcacgttc agggcatagc cgacgccatg accggtgccg tgaccgtagt
     1561 cgacgccccc ggcccagatc ggcgcgcgcg ccagcgtgtc gagcagcggc gacctcgtgc
     1621 cgcgcggaaa caccgccatc gacagctgga tcatcccctt gagcacgagc gtgtagtcgc
     1681 gctgctgctc cgccgatgtc gttccgatcg gcacgacccg cgtgatgtcg gtggtgccgt
     1741 aatcgtactg cgcgccggag tcgatcagca gcaacccgtt gccgggcgaa cccgcggcgc
     1801 tgatcaccac gtgcgactcg gcggtggcgc ggtagtgcgg cagcgcgccg ttggcgttga
     1861 agccggcgat cgtcgagaag ctcggcgaca cgaacccggg ccgctgcgcg cgcgctgccg
     1921 tgatgtgctc gtcgatcgtc agttcggtga tccgctcgcc ctgcgcgagc gccgcttcga
     1981 gccaggtgaa gaattcacac agcgccgcgc cgtcctgctc catcgcgcgc cggacgtgcg
     2041 cgatctcggc gtcggtcttg cggctcttcg cgagcgtgga cggattcagc gcctcgacaa
     2101 tgcgcacatg ggccggaacc tgtgcgcgca tcccgagcgt cacgcgcttg ggatcgatca
     2161 gcagcgccgc gttgcgcggc aggttcgcga gtgccgcggc ggcccactcg tacggccgca
     2221 cgtcgacgcc ttcgccctcg aggcggtcgg caagccccgg cggcaccttg ccttcgccga
     2281 cgaacagcgt ggcgcgctgc ggcccgatca gggcgtgcgc gacgaacacc gggttgtacg
     2341 gcacgtccgc accgcgcagg ttgaacagcc acgcgatgtc gtcgagcgtg gaaatcaggt
     2401 gccagtcgac gccggccgcc tgcatctgcg cgcgcacggc ggcgagcttg tccgggcgcg
     2461 ccaccggcgc gtgcgcgcgc gcgtgctcgc gcacgggtgc ggcaggcaag ggcggacggt
     2521 cacgccacac ccggtcgagc aggtcgaggt cggtgcgcag tttcacgcga cgcggcgcga
     2581 gtctgtcctc gagcgcgcgg gactgcgcca atgacaggac cgcgccgtcg acgcccaccg
     2641 ttccaccggc cggcacgtgc tcggccagcc agtcgatgtg cgcgctgctg gtcgcgccgc
     2701 cgatgcgcat caccgtgatg ccggtgccgg cggtctgcgt ctcggcctgc acccagtagc
     2761 gactgtcgac ccacagtccg gcgaagtccg cggtgacgat cagcgagccg accgaccccg
     2821 tgaagcccga cagccactgg cgtccctgcc agcgttcggg cagatattcg gacaggtgcg
     2881 gatcggccga cggcaccagc caggcggcga tcgattcttc ctgcatcgca gcgcgcagcg
     2941 catcgatgcg ggcccggatg tcggaacggt gagcagtgag cgtatccatc tgcgtcggag
     3001 cggaaaccgg agtggctgcg cagtctacgc cgcgaccatc ggcgcaccga tggtcggttc
     3061 cggccggcct aatacgcatg catgaaagac aaggccgaac ggcaggacta ccttgagcac
     3121 ctgcagacga atgcacagcg gccgcccggt gctccgtcct ccaagcccgg ccccggagac
     3181 caccacgcat cgtctgcaac catcttcatc tgtgttctcc ccgacctcgc cgcgtgtacc
     3241 tcacgcggct tttttttgcc cgggcgtgcg ctccttctcg ttgcggccag cctcgcgcat
     3301 gggtcgccgc agctgccaca acccgtcgac cgcgaagatc gccagcgcga tccagatcag
     3361 cgcatagccg gcaagcttcg atgccgcgaa cggctcgtga aagacccaca cgccgagcgc
     3421 gagctgcagc gtcggcgaga tgtactgcag gatgcccagg tgtgcgaacg gaatgcggcg
     3481 cgcactggcg gcaaacagca gcaacggcgt ggcagtcacg ggtcctgccg ccgcgagcag
     3541 ccagcgcgtg gcgtcggtgc cgggcgtgaa cgcgtcctgc ccttgcgccg ccagccagag
     3601 cagatacgcg agcgcgaacg ggaacagcag tgccgtttcg agcgcaagcc cttcgatcgc
     3661 gcccagcgcg gcggtcttgc gcagcagccc gtacgcgccc caggtgagcg cgagcgccag
     3721 gccgatccac ggcggcgcac cggcgtccca ggtcagccag gccacacctg ccgccgcgac
     3781 cgacaccgcg gtccattgcg ggcggcgcag tcgttcttgc agcacgaagg cgccgatcag
     3841 cacgttcacc agcgggttga tgtaatagcc gaggctggcg tcgatcacgt ggtcggcacc
     3901 gatggcccag atgtacgtgc cccagttcac cgtcaccagc agcgcgctga cgacgaaagc
     3961 cgccagcacg cgcggtcgtt gcgcgaggat gcgcagccat tgccagcggc gcagcgcggc
     4021 cagcagcagc gcgcataccg cgagcgacca cgccatccgg tgtgccagga tctccggcgc
     4081 cggcacgccg tgcagtgcct tgaagtacag cggcacgacg ccccacacga agtaggcgcc
     4141 gagcgcatac aggatgccgc gattcaccgg gtgcccccac gcatggcgcg cattatggcg
     4201 gccggcgcct ccggcaacca aatggttgac aaaagcaacg atcggcggga aagtcggcgt
     4261 gcatgacgga ccgccagctc gaagacgacg tggcgcgggt acgcgccttc aaccgcttct
     4321 acacgcagac gatcggcgtg ctcgacgagg gtctgctcga cagctccttc tcgctgaccg
     4381 aggggcggct gttgtgggaa ctcgcgcacc gcgacgacct gacggccggc gagttggcgc
     4441 gcgcgctgaa tctcgacccc ggctacgtca gtcgcatgct ggcgcgcttc cgggggcggg
     4501 gcctggtcgc gcgcgaggtg tcggccaccg accggcgccg ccggcatctg gcgctgacga
     4561 agcgtggcgc gcgtgcattt gcgccgctgg accggcgcgc gcatgacgaa gtcgccgcgc
     4621 tgctcgggcg gcttgcgccc ggcgagcggc ggcgcctgct gcagtcgctg cagacggtcg
     4681 aatcgctgct cggcgcgcgc accgagcgcg tcgctccgta cgtgctgcgg ccccatcgtg
     4741 ccggcgacat cggctgggtc gtcgcgcgcc acggggcgct gtaccacgag gagtatggct
     4801 gggacctcac cttcgaggcg ctggtggccg agatcggagc ggaattcctg cgcaacttcg
     4861 atccgcagcg cgagcactgc tggatcgccg agcgcgacgg cgttccggtc ggttcggtgt
     4921 tctgcgtcgc gcacagccga cgcgtggcca agctgcggct gctgctggtg gagccggcag
     4981 cgcgcgggtc gggcctggga gccgcgctgg tggccgagtg catccgcttc gcacgcgcgg
     5041 ccggctatca cacgttgacg ctgtggacca acgacgtgct ggtggccgcg cgccgtatct
     5101 acgagcgctg cggattccgt ttggtgcggc aggagaagca ccacagcttc ggccacgacc
     5161 tggtagggca gtactggaaa ctgccgctgc ggtgacgcgc ctcgcgccgc tgcttgccgc
     5221 ggccgcgttc ggcgtgcagg tcggcgctgc gatcgtcgcc acgcgcttcg tggtcggcga
     5281 gacgacgccg gcggcgct
//
LOCUS       DBNHBY010000718         2421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_863948, whole genome shotgun sequence.
ACCESSION   DBNHBY010000718 DBNHBY010000000
VERSION     DBNHBY010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_863948"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="AC16HB_16970"
     CDS             complement(<1..1057)
                     /locus_tag="AC16HB_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR5608536.1"
                     /translation="MGQPQKKSVAALTVAAIGVVYGDIGTSPLYTVKEIFSESTGVAL
                     TAANLIGAVSAIFWLLMLVVTLKYVVLILRADNRGEGGIMALVALAASAAGRTPRRRA
                     ALLLVGAFGASLFYGDGVITPAISVLSAVEGLEVITPRLEPYVLPISIGILVGLFIVQ
                     RHGTAVVGRWFGPTVIVWFATLAIAGAVQIARAPQILQALNPLHAIEFLAARGPGIFA
                     TVGAVVLAVTGAEALYADLGHFGKRPIRIAWLGLVFPALAINYLGQGALLIAQPSALD
                     NPFYRLFPESLLLPAVVLASIATVIASQAVISGAYSMTQQAIHLGFLPRMAITYTSAK
                     EMGQIYMPAVNWMLLAAV"
     gene            complement(1152..>2421)
                     /locus_tag="AC16HB_16975"
     CDS             complement(1152..>2421)
                     /locus_tag="AC16HB_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR5608537.1"
                     /translation="DAINVLTRHLDEPGTTMFPKAPAFAANTRGAPGQGRGVTIGRHA
                     SRVSGAPEVFGELPMNCLAEEIETPGAGQLRALVTVASNPVLSAPNGPRLAAALESLD
                     FMVSVDVYVNETTRHADVILPGTSPLEEMHWDAAFPQLAYRNCARASAAVFPPPGGRP
                     AEWEILLRLTAIAGGRAEADLARLDDELASADVKRAAGEHAAAVIKAVAHRRGPERLI
                     DLALRAGPYGDRFGMKPDGLNLDKVQAAADGIDLGPLAPRIPELLRTPSGRIELAPPL
                     LLADLARAQAALDAPAPEFVIVGRRQVRSNNSWMHNLPTLAKGRNRCTVLVHPTDAQR
                     LGLNDGGRARVARGANAIEAQVEVTDEMMPGVISLPHGWGHDLSDARLSVAAVQPGAN
                     LNAVLDDRARDPLSGNAVLSGVAVSVAAIA"
BASE COUNT          375 a          869 c          837 g          340 t
ORIGIN      
        1 gcaccgccgc gagcagcatc cagttgacgg cgggcatgta gatctgcccc atttccttcg
       61 ccgacgtgta cgtaatcgcc atgcgcggca ggaagccgag atggatcgcc tgctgcgtca
      121 tcgaatacgc gccggagatc accgcctgcg aggcgatcac ggtggcgatc gacgccagca
      181 ccactgcggg tagcagcagc gactcgggga acagccggta gaacgggtta tcgagcgcgc
      241 tcggctgcgc gatcagcagc gcgccctggc ccaggtaatt gattgcgagc gccggaaaca
      301 cgaggcccag ccacgcaatc cggatcggcc gcttgccgaa gtggccgagg tcggcataca
      361 gcgcctcggc gccagtcacc gcgagcacga cagcgccgac agtggcgaag atgccggggc
      421 cgcgcgccgc gaggaactcg atcgcgtgca gggggttgag cgcctgcagg atctgcggtg
      481 cgcgcgcgat ctgcaccgcg ccggcgatcg ccagtgtcgc gaaccagacg atcacggtcg
      541 gtccgaacca gcgaccgacc accgcggtgc cgtgccgctg cacgatgaac agccccacca
      601 ggatgccgat cgagatcggc agcacgtagg gctccagccg cggcgtgatc acctcgagcc
      661 cctcgaccgc gctgagcacg gagatcgccg gcgtgatcac gccgtcgccg tagaagagcg
      721 acgcgccgaa cgcgcccacc agcagcagcg cggcacggcg gcgcggcgtc ctgcccgcgg
      781 cagaggccgc aagcgcgacc agcgccatga tgccgccctc gccgcggttg tcggcgcgca
      841 ggatcagcac cacgtacttc agcgtgacga cgagcatcag cagccagaag attgccgaca
      901 ccgcaccgat caggttcgcc gccgtcagcg caacgccggt cgattcgctg aagatctcct
      961 tgacggtgta gagcgggctg gtgccgatgt cgccgtacac gacgccgatc gccgccaccg
     1021 tcaaggcggc cacgctcttc ttctgcggtt gtcccacgac gccctcgtcg ctggtccggg
     1081 gactcccttt ctaggcgtgg cgccgtaaag agcgcataaa aaaagcgcgg cgacgcgtcc
     1141 cgccgcgccg ctcaggcgat cgcagccacc gacacggcta ctccactgag caccgcgttg
     1201 cccgacaagg gatcgcgcgc gcggtcgtcg agcacggcat tcaggttcgc gccgggctga
     1261 accgccgcca cgctgagccg cgcgtccgac aggtcgtggc cccacccgtg cggcagcgag
     1321 atgacgcccg gcatcatctc gtcggtcacc tcgacctgcg cctcgatcgc gttggcgccg
     1381 cgcgcgacgc gggcgcgtcc gccgtcgttc aggccgagcc gctgtgcatc ggtcgggtgc
     1441 accagcaccg tgcagcggtt gcgccccttc gcgagcgtgg gcaggttgtg catccagctg
     1501 ttgttcgagc gcacctggcg gcggccgacg atcacgaact cgggcgctgg cgcatcgagc
     1561 gctgcttgcg cgcgcgcaag atcggcgagc agcagcggcg gcgcgagttc gatgcgtccc
     1621 gacggcgtgc gcagcagttc gggaatgcgc ggcgcgagcg ggccgaggtc gatgccatcc
     1681 gccgctgcct gcaccttgtc cagattcaga ccgtccggct tcatcccgaa acggtcgccg
     1741 tacggtccgg cgcgcagcgc gaggtcgatc aaccgctccg ggccgcgccg gtgtgccacc
     1801 gccttgatca ccgcggcggc atgctcgccg gcggcccgct tcacgtcggc ggacgcgagt
     1861 tcatcgtcga gccgcgcgag atcggcctcg gcccgaccac ccgcgatcgc cgtgagccgc
     1921 agcaggattt cccattcggc cggccgcccg ccggggggcg ggaataccgc ggcgctcgcg
     1981 cgcgcgcagt tgcgatacgc gagctgcggg aacgcggcgt cccagtgcat ctcttcgagc
     2041 ggcgacgttc ccggcaggat cacgtcggcg tggcgcgtcg tctcgttcac gtacacgtcg
     2101 acgctgacca tgaagtcgag cgactcgagc gcggcggcga ggcgcggtcc gttgggcgcc
     2161 gacagcaccg ggttgctcgc gaccgtgacc agcgcgcgga gctggccggc acccggcgtt
     2221 tcgatctctt ccgcgaggca gttcatcggc agttcgccga acacctcggg cgcgcccgat
     2281 acgcggctcg cgtgccggcc gatcgtcacc ccgcgcccct ggcccggcgc gccgcgcgta
     2341 ttcgccgcga acgcgggtgc cttcggaaac atcgtcgtgc cgggctcgtc gaggtggcgc
     2401 gtcagcacgt tgattgcgtc g
//
LOCUS       DBNHBY010000719         3697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1203421, whole genome shotgun sequence.
ACCESSION   DBNHBY010000719 DBNHBY010000000
VERSION     DBNHBY010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1203421"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16HB_16980"
     CDS             complement(<1..279)
                     /locus_tag="AC16HB_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608538.1"
                     /translation="MSGLLQRRAQVSVAVTLAIAVLLLLVAGQYAFFKRAWAKEKLAE
                     TEPRYARLLGLRGAGPQLDVALKDARDAVARLGYPAQRDAAQVGNDLQQ"
     gene            complement(276..1013)
                     /locus_tag="AC16HB_16985"
     CDS             complement(276..1013)
                     /locus_tag="AC16HB_16985"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR5608539.1"
                     /translation="MRQAAEFDARAASPFAESDLAWGFVVDRGERERTRVDIALTSRR
                     MVDEQLAAHRDRLGGAMPEVWADGDRPIVIPGYGEAQRSAREHKSRRLLLLLLVLAAL
                     QAGALALTPTLQLRAKAIAAKTSLDELMRKVQPQAKTRDELAKLEEQSRLLGRAAQNR
                     PDVLLLLDEVTRRLPDDAVLSRFEISGNTVRISGQADNAAQLLQTLGALPEFRDVRAP
                     GGIARAQAGGKESFTIEFSVAAGAKSS"
     gene            complement(1312..2220)
                     /locus_tag="AC16HB_16990"
     CDS             complement(1312..2220)
                     /locus_tag="AC16HB_16990"
                     /inference="COORDINATES: protein motif:HMM:NF045433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin"
                     /protein_id="HIR5608540.1"
                     /translation="MKSQMRTWLPCRQEGIALIAVLWIVAALTVIVMGAVHASRNEVR
                     TVSAFKEMSVAGALTDAGTVLVARQLAASPARDGVLRSFEATLEGQLVHIEVIPLVGL
                     VDLNGASEALLTDLISVAGGVERGEANVLAQRIIDWRDPDDQPHSAGAEDAAYIAAGS
                     PFRTRSGPFEVPEDLLQVLGVDFDLYVKLRRLVTVHLRGGGRISPAAAPLPVLQVLAR
                     GNEQFALDYASARDARGLLADTTRFPADYITQTASSRFLLEASTRLFNGASVVTRKVV
                     DVSSWQEGVPWTILWSERFVEPSAAR"
     gene            complement(2226..2897)
                     /locus_tag="AC16HB_16995"
     CDS             complement(2226..2897)
                     /locus_tag="AC16HB_16995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5608541.1"
                     /translation="MTERCANRGFTLVELLVALALLSIVMLALGTAMRSFAQTEVRID
                     QHMQRDEEFRVAVNFLRTILAEMSPLAVRTAPGAPKRIAFAGAGDQLDWIGVMPARQG
                     AGGLYRFRLFVRSGTDAPAALILDFAPLTDFDSPLDSGAPQSRVMVDGVSGVAFRYED
                     TTAPEAAWLSAWPYPDRLPRRVGLQIRTSAQTWPELIVTVLPAVGPGVSSRAGGGSGP
                     IIGPF"
     gene            complement(2894..3295)
                     /locus_tag="AC16HB_17000"
     CDS             complement(2894..3295)
                     /locus_tag="AC16HB_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR5608542.1"
                     /translation="MSPTSHRIHGFALLEVVVALAILAPSFGVLYRAIGGSVRAVGDT
                     SRYAHAIVLAESILQSLDAVPAGGWNESGALEAFQWAVTSAPYDAAGDAGVPLYRVQV
                     AVGWNDGLRERTFALVGLLPERGKFATGLTQ"
     gene            complement(3292..>3697)
                     /locus_tag="AC16HB_17005"
     CDS             complement(3292..>3697)
                     /locus_tag="AC16HB_17005"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR5608543.1"
                     /translation="VLAIVGLLLSVAPFAFQRYRESAEYRDTVRTMTADLSAARQLAM
                     TSGRDVAFSVDLGQRQYGIEGLPPRTLPSGVDVRVTVADVEFTDRVARIRFYPGGNAT
                     GGNVDIVRASGTGVRLRADWLDGRITMQGLAQ"
BASE COUNT          673 a         1283 c         1180 g          561 t
ORIGIN      
        1 ctgctgaaga tcgttcccga cctgggccgc atcgcgctgc gcgggatacc ccagacgcgc
       61 gaccgcgtcc ctggcatcct tcagcgccac gtcgagctgc gggcctgcgc cgcgcaggcc
      121 gagcagccgc gcgtaacgcg gctccgtctc ggccagtttt tccttcgccc aggcgcgctt
      181 gaagaaggcg tactggccgg ccacgagcag caacagcacc gcgatcgcca gcgtgactgc
      241 gaccgagacc tgggcacgac gctgcagcag gccgctcatg acgatttcgc tcctgcagcg
      301 acgctgaatt cgatggtgaa gctttccttt ccacctgcct gcgcgcgcgc aatgcccccc
      361 ggcgcacgga cgtcgcgaaa ctccggcagt gcgccaagtg tctgcagcaa ctgagccgcg
      421 ttgtccgcct ggccggaaat gcgcaccgta ttgccgctga tttcgaagcg cgacagcacg
      481 gcgtcgtccg gcaatcggcg cgtgacctca tcgagcaaca aaaggacgtc cggccgattc
      541 tgcgcggcac ggccaagcag ccggctctgc tcttcgagct tggccagctc atcgcgcgtc
      601 tttgcctgcg gctgcacctt tcgcatcaac tcgtcgaggc tggtcttggc cgcgatcgcc
      661 ttggcgcgca attgcagtgt gggcgtgagc gcgagcgcgc ccgcctgcag tgccgcgagc
      721 acgagcagca gcaggagcag gcgccgcgac ttgtgctccc gcgccgagcg ctgcgcttct
      781 ccatagcccg gtatcacgat cggtcgatcg ccgtcggccc agacttccgg catcgctcca
      841 cccagacggt cgcggtgcgc cgcgagctgc tcgtcgacca tccgtcgaga ggtcagcgcg
      901 atgtcgacgc gagtccgctc gcgctcgccg cggtcgacga cgaaacccca tgccaggtcg
      961 gattctgcaa acgggctcgc agcgcgcgcg tcgaattctg ccgcctggcg catctcggca
     1021 ctcgacaaac gcggaagcga aagcgtcctg cggagcacac aatcttccgc gagcgcgatg
     1081 gcgaggaatg cagcgcctgg atcgccgctc gcgcgcttcg ccgtagcgcc gctgcgcagg
     1141 gattcgctgc cgtctgcatg caggactcgc acgacagcgg cgggcgtgag ccagcgcagc
     1201 gccggccagc gcagcgcctc ggaccagcca tcgcgaaggt attccacgac cgcggcgaga
     1261 tcgatcccga acagtcggag actgtcgcgc gatttcgctg cggtcagcag actaccgggc
     1321 tgcactgggc tcgacaaagc gctcgctcca cagaatggtc cacggaaccc cttcctgcca
     1381 cgacgaaacg tccacgacct tccgggtcac gacgctggcg ccgttgaaca atcgggtcga
     1441 cgcttccagc agaaagcgtg atgacgcagt ctgcgtgatg tagtccgctg gaaatcgagt
     1501 cgtgtccgcc agaagtccgc gcgcatcccg cgcacttgca tagtcgaggg cgaactgttc
     1561 gttgcccctc gccagcacct gcagaacggg aagcggtgcg gcagccgggc tgatgcgccc
     1621 gccgcctctc aagtggacgg ttacgaggcg gcgcagctta acatagaggt cgaaatcgac
     1681 gcccagaacc tgcagcagat cttcgggcac ctcgaacggt ccgctgcgag tgcgaaatgg
     1741 agaccccgcg gcgatgtatg cggcgtcttc ggcgcctgcg gaatggggtt ggtcgtccgg
     1801 gtcgcgccag tcgatgatcc gctgcgcgag gacatttgcc tcgccgcgtt caacgccgcc
     1861 ggcgacgctg atcaggtcgg taagcagagc ctcggacgcg ccattcaggt caaccaggcc
     1921 cacgagggga atcacctcaa tgtgaaccaa ttggccttcg agcgttgctt cgaagctgcg
     1981 caacacgccg tcgcgcgccg gtgacgccgc gagttgccgc gccacgagga cggtgcccgc
     2041 atcggtaagc gcgcccgcaa cgctcatttc cttgaaggcc gaaaccgtgc gcacctcgtt
     2101 gcgcgatgcg tggactgcgc ccatcacgat gacggtcagc gcggcgacga tccagagcac
     2161 cgcgatcagg gcgatgcctt cctggcggca cgggagccaa gtgcgcatct gcgacttcat
     2221 gacgcctaga agggcccgat gattggaccg gacccgccgc ccgcacgcga cgagacgccg
     2281 ggtccgacgg ccggcaacac cgtgacgatg agttccggcc acgtctgcgc agatgtcctg
     2341 atctgcagtc cgacgcgccg cggcaggcga tccggatacg gccaggcgga tagccaggcc
     2401 gcctccggcg ccgtggtgtc ctcgtagcga aaggccacgc cggaaacgcc atcgaccatc
     2461 acgcgcgact gcggggcgcc gctgtccagg ggagagtcaa aatctgtcag aggcgcgaaa
     2521 tcgaggatca gcgctgcagg ggcatccgtg cccgaccgca cgaacaatcg aaagcgatac
     2581 aggcctccgg cgccttggcg ggccggcatc acgccgatcc agtcgagttg atcgccggca
     2641 ccggcgaagg cgatccgctt cggcgcgccc ggtgcggtgc gcacggccag cggagacatt
     2701 tcggccagaa tcgtccgcag gaagttcacg gcaacgcgga actcctcgtc acgctgcata
     2761 tgctgatcga tccgcacttc ggtttgcgcg aaggaccgca tcgccgttcc aagtgccagc
     2821 atcacgatgg atagcaatgc gagcgccacc agcagttcga ccagcgtgaa tccgcggttc
     2881 gcgcagcgct cggtcattgc gtaagacccg tggcgaactt gccccgctcc ggcagcagac
     2941 cgaccagggc gaaggttcgc tcccgcaagc cgtcgttcca accgaccgcc acctgcactc
     3001 tgtagagagg cactccggca tcgcccgcag cgtcatacgg ggcgctcgtc accgcccact
     3061 gaaacgcctc cagagcacca ctctcattcc atccacccgc gggcactgcg tccagcgact
     3121 ggagaatcga ttcggccaat acgattgcgt gcgcatagcg cgatgtgtcg cccacggcac
     3181 gcacgctgcc gccgatcgcc cggtacagca cgccgaacga aggcgccagg atggcgagcg
     3241 ccacgaccac ctcgagcagc gcaaagccat ggatccgatg cgatgtcgga ctcactgtgc
     3301 gagcccctgc atcgtgatcc gcccgtcgag ccagtcggca cgaagtcgca caccggtgcc
     3361 ggaggcacgc acgatgtcga cgttgccgcc agtcgcattg ccccccgggt agaagcggat
     3421 gcgcgcgaca cgatcagtga actcgacatc ggcgaccgtt acacgaacgt cgactccgga
     3481 gggcaacgtc cgcggcggca gtccctcgat gccgtactgg cgctggccca gatccacgct
     3541 gaaggcaacg tcgcgcccgc tcgtcatggc gagttggcgc gcggcggaga gatccgcagt
     3601 catcgttctg acggtatccc ggtactcggc cgactcgcga tagcgttgaa aggcaaatgg
     3661 cgccaccgag agcaggagcc cgacgatcgc cagcaca
//
LOCUS       DBNHBY010000720         5019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_527406, whole genome shotgun sequence.
ACCESSION   DBNHBY010000720 DBNHBY010000000
VERSION     DBNHBY010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_527406"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..845)
                     /locus_tag="AC16HB_17010"
     CDS             complement(45..845)
                     /locus_tag="AC16HB_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR5608544.1"
                     /translation="MSLEALIFDVDGTLADTEEAHRQAFNAAFDEHGVRWHWTRSRYA
                     ELLGVSGGRERMLLHLESLPDSPAAKARQREQIPEIHRSKVRIFRSLVEAGRVPLRAG
                     VKRLIDEARAAELKLAIATTGTASGVAALIAHTLGEDAPGWFQVFAAGDQVAQKKPAP
                     DVYHLVLRTLHVPSSACVAFEDSGNGVRAAHAAGLFVVATPSFWTLDQDFSQADLLLP
                     TLGDPGRPIPREIAQRHARGAEFVTLALLEKLHAAAAERAQAGAKYAA"
     gene            complement(877..2931)
                     /gene="tkt"
                     /locus_tag="AC16HB_17015"
     CDS             complement(877..2931)
                     /gene="tkt"
                     /locus_tag="AC16HB_17015"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5608545.1"
                     /translation="MNAPQKFSERAAAARLRANALRFLAIDAVNAAKSGHPGMPMGMA
                     DIAEVLWRRYLKHDPTDPAWADRDRFVLSNGHGSMLLYALLHLTGYDLPLEELKNFRQ
                     LHSKTPGHPEAGLTPGVETTTGPLGQGLANAVGMALAEKLLAARFNQPGHAIVDHRTW
                     VFVGDGCLMEGISHEACSLAGVFGLDKLVCFYDDNGISIDGHVKGWFRDDTAARFRAY
                     GWNVVGPIDGHDALAIECAIDTALAERARPTLVICRTTIGWGAPKAAGTHEVHGAPLG
                     ADETAATRAALGWPHAPFDVPAHLRAEWDARAQGASLQREWQHRFAAYAQAHPALAQE
                     FARRMRGELAADWPQIARKLLDGARGVTAPTATRKSSQNALESLVPAVPELLGGSADL
                     TWSNLTAVKASAPIDGFGAGNYVHYGVREFGMGALMNGLALHGGFIPYGGTFAVFTDY
                     ARSALRMSALMKQRVVYVLTHDSIGLGEDGPTHQPVEHAASLRVIPNLDVWRPCDGVE
                     TAVAWRAGVERKSGPTALLLSRQNLPALAREADEAAVARGGYVIADAEGPLAVVLIAT
                     GSEVELALKARAQLAAEGIGARVVSMPSTSVFDRQDRAWQSRVLPRGVPRVAIEAAHP
                     DIWRKYVGLEGDVVGLDRFGESAPAGKLYDYFGITAARVAHCARALVGARTADARVAE
                     PA"
     gene            complement(2940..3812)
                     /locus_tag="AC16HB_17020"
     CDS             complement(2940..3812)
                     /locus_tag="AC16HB_17020"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HIR5608546.1"
                     /translation="MSIKHPIIAVTGSSGAGTTTVMKSFQHIFRRDGIKAQTVEGDSF
                     HRYDRRAMREKMKQAEAAGNRHLSHFGPDNNLFVELEQLFRQYGEKGRGRVRKYLHDA
                     GEAEPYKQEPGTFTPWDDIPADTDLMFYEGLHGGYVGPEADIARHVDLLVGVVPIINL
                     EWIQKLHRDKSLRGYSQDAVVDTILRRMPDYVNYICPQFSRTHVNFQRVPTVDTSNPF
                     IAADIPSADESMVVIRFANPKGIDFPYLLSMLHDSWMSRPNNIVVPGGKMGLAMQLIF
                     TPMILRLIDLKKRA"
     gene            complement(3845..4924)
                     /locus_tag="AC16HB_17025"
     CDS             complement(3845..4924)
                     /locus_tag="AC16HB_17025"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR5608547.1"
                     /translation="MHHGRTTLSKFLIQQLHDVPDHGKLAGLLVDVAAAIKAISAMTA
                     RGALGASHGAAAARNASGDEQQPLDMQSNETMLRHCEWGGLVAGMASEELDTPYALPD
                     EYPRGPYLLVFDPLDGSSNIDVNVSVGTIFSVLRHAGSAAPTDKDYLQKGTEQVAAGC
                     AIYGPATMLVLTVGKGTHGFTLDREIGNFILTHSNLRIPEDTSEFAINTSNERFWEPP
                     VTRYVSECKAGRTGIRERDFNMRWIASMVAEAHRILMRGGVFMYPKDTRDPTKAGRLR
                     LMYEANPIGMVIEQAGGMASTGRGRILEVAPTELHQRVPVILGSKNEVARIERYHREY
                     DRGEDKPFISPLFNERSLYRPEARA"
BASE COUNT          730 a         1721 c         1723 g          845 t
ORIGIN      
        1 gaatcggatc ggcggtggcg gccatgcggg cctccgtgtg aaagctacgc cgcgtatttt
       61 gcgccggcct gggcgcgctc ggccgcggcc gcgtgcagct tctccagcag cgcgagcgtg
      121 acgaactccg cgccacgggc gtggcgctgc gcgatctcgc gtgggatcgg ccggcccgga
      181 tcgccgagcg tgggcagcag cagatcggcc tgcgagaagt cctgatcgag cgtccagaag
      241 cttggcgtgg cgacgacgaa cagcccggcc gcatgggccg cccggacgcc gttgccggag
      301 tcctcgaacg cgacgcacgc cgacgatggc acgtgcagcg tgcgcagcac gaggtggtac
      361 acgtcgggcg cgggcttctt ctgcgccacc tggtcgccgg ccgcgaagac ctggaaccag
      421 ccgggcgcgt cctctcccag cgtgtgcgcg atcaacgccg cgaccccgct cgcggtgccg
      481 gttgtcgcaa tggcgagctt cagttccgcg gcacgcgcct catcgatcag ccgcttgacg
      541 ccggcgcgca acggcacgcg gcccgcctcg accagcgagc ggaagatccg cactttgctg
      601 cgatggatct ccggtatctg ttcgcgctgt cgcgcttttg ccgccggcga atccggcagt
      661 gattcgaggt gcagcagcat gcgttcgcgc ccgcccgaca cgcccagcag ttcggcgtag
      721 cggctgcggg tccagtgcca gcgcacgccg tgctcgtcga acgcggcatt gaaagcctgg
      781 cggtgcgcct cctccgtatc cgcgagcgtc ccgtccacat cgaagatcag cgcctcgagg
      841 ctcattgctt gttccttccc gtctgcgggc ggcgcttcac gccggctccg ccacgcgtgc
      901 atcggccgtc cgcgcgccga ccagcgcacg cgcgcagtgc gcgacccggg ccgcggtgat
      961 gccgaaatag tcgtagagct tcccggccgg cgccgattca ccgaagcggt cgagcccgac
     1021 cacgtcgcct tcgaggccga cgtacttgcg ccagatgtcc gggtgagcgg cctcgatcgc
     1081 gacgcgcggc acgccgcgcg gcagcacgcg gctctgccat gcacgatcct gccggtcgaa
     1141 gacgctggtc gacggcatcg ataccacgcg cgcgccgatg ccctcggcgg cgagctgcgc
     1201 gcgcgccttc agcgccagtt cgacctcgga accggtggcg atcagaacga cggcgagcgg
     1261 cccttccgcg tctgcgatca cgtaaccacc gcgcgcgacc gccgcctcat cggcttcgcg
     1321 cgccagcgcc ggcaggttct gccgcgacag caacagtgcg gtcgggccgc tcttgcgttc
     1381 gacaccggcg cgccatgcga ccgcggtttc gacgccgtcg cacggacgcc agacatcgag
     1441 gttcgggatc acgcgcaggc tggccgcgtg ctcgaccggc tggtgcgtcg gcccgtcctc
     1501 gcccagtccg atcgaatcgt gcgtgagcac gtagacaacg cgctgcttca tcagcgcgct
     1561 catgcgcagc gccgagcgcg cgtagtcggt gaagaccgcg aacgtgccgc cgtaagggat
     1621 gaagccgccg tgcagcgcga gcccgttcat cagcgcaccc atgccgaact cgcgcacgcc
     1681 gtagtgcacg tagttgccgg caccgaagcc gtcgatcggc gccgaggcct tcaccgcggt
     1741 cagattcgac caggtgagat cggccgagcc gccaagcagt tcgggcactg ccggcaccag
     1801 ggactcgagc gcgttctgcg acgacttgcg cgtggccgtg ggcgctgtca cgccgcgcgc
     1861 gccatccagc agtttgcgtg cgatctgcgg ccagtcggcc gccagctcgc cgcgcatgcg
     1921 gcgcgcgaac tcctgcgcca gcgcagggtg cgcctgcgca taggcggcga agcggtgctg
     1981 ccactcgcgc tgcagtgacg ccccctgcgc gcgcgcgtcc cactcggcgc gcaggtgcgc
     2041 cggcacatcg aacggcgcgt gcggccagcc gagcgcggcg cgcgtcgccg cggtttcgtc
     2101 ggcccccagc ggcgcgccgt gcacttcgtg tgtgccggcc gccttcggcg cgccccagcc
     2161 gatcgtggtg cggcagatca cgagcgtcgg ccgcgcgcgc tccgcgagcg cggtgtcgat
     2221 cgcgcattcg atcgcgagtg cgtcgtggcc gtcgatcggg ccgaccacgt tccagccgta
     2281 cgcgcggaag cgggccgcgg tgtcgtcgcg aaaccagccc ttcacgtggc cgtcgatcga
     2341 gatgccgttg tcgtcgtaga agcagaccag cttgtccagg ccgaacacgc ccgcgagcga
     2401 acatgcctcg tgcgagatgc cttccatcag gcagccgtcg ccgacgaaga cccaagtgcg
     2461 gtggtcgacg atcgcatgtc ccggctggtt gaagcgcgcc gcgagcagtt tctctgcgag
     2521 cgccatgccg accgcattcg ccaggccctg gccgagcggc cccgtcgtcg tctccacgcc
     2581 gggcgtcagc cccgcctcgg gatgccccgg ggtcttcgaa tgcagctggc ggaagttctt
     2641 cagctcttcg agcggcaggt cgtagccggt caggtgcagc agcgcgtaca gcaacatcga
     2701 gccgtggccg ttcgacagca cgaagcggtc gcgatcggcc cacgccggat cggtggggtc
     2761 gtgcttcaga tagcggcgcc acagtacctc ggcgatgtcg gccatgccca tcggcatgcc
     2821 ggggtgcccc gatttggcgg cgttgaccgc gtcgatggcg agaaagcgca gcgcgttggc
     2881 gcgcagccgc gcggcggcgg cacgctcgga gaatttctgg ggtgcgttca tcgtttcctc
     2941 taggcgcgct tcttcaggtc gatcaagcgc aggatcatcg gcgtgaatat cagctgcatc
     3001 gccaggccca tctttcctcc gggcaccacg atgttgttcg gccggctcat ccacgagtcg
     3061 tgcagcatcg acagcaggta gggaaagtcg atgcccttcg gattggcaaa tcggatcacg
     3121 accatgctct cgtccgcgct cgggatgtcg gccgcgatga acgggttgga cgtatccacc
     3181 gtcggcacgc gctggaagtt gacgtgcgtg cgcgagaact gcggacagat gtagttcacg
     3241 tagtcgggca tgcggcgcag gatcgtgtcg acgaccgcat cctgcgaata gccgcgcagg
     3301 ctcttgtcgc ggtgcagctt ctggatccac tcgaggttga tgatcggcac cacgccgaca
     3361 agcaggtcga cgtggcgcgc gatgtccgcc tcgggtccga cgtagccgcc gtgcagtccc
     3421 tcgtagaaca tcaggtcggt gtcggccggg atgtcgtccc acggcgtgaa cgtgccgggc
     3481 tcctgcttgt acggctcggc ttcgccggcg tcgtgcaggt acttacgcac gcggccgcgg
     3541 cctttttccc cgtactggcg gaacagctgc tcgagctcga cgaacaggtt gttgtcgggc
     3601 ccgaagtgac tgaggtgccg gttgccggcc gcctcggcct gcttcatctt ttcgcgcatc
     3661 gccctgcggt cgtagcggtg gaacgaatcg ccctcgaccg tctgcgcctt gatgccgtcg
     3721 cgccgaaaga tgtgctggaa actcttcatc accgtcgtcg tcccggcgcc gctggacccg
     3781 gtgacggcga tgatcggatg cttgatcgac actgcgtcct ccctcgttac ctcgatacgc
     3841 gccttcacgc cctcgcttcg gggcggtaga gcgagcgctc gttgaacagc ggcgaaatga
     3901 acggcttgtc ctcgccgcgg tcatactcgc ggtgatagcg ctcgatgcgt gcgacttcgt
     3961 tcttcgagcc gagaatcacc ggcacgcgct gatgcagttc ggtcggcgcg acttcgagga
     4021 tgcggccgcg gccggtggac gccatgccgc cggcctgttc gatcaccatg ccgatcggat
     4081 tggcttcgta catcagtcgc aggcggccgg ccttggtcgg atccctggtg tccttcgggt
     4141 acatgaacac gccgccgcgc atcagaatcc ggtgcgcctc tgccaccatc gacgcaatcc
     4201 agcgcatgtt gaagtcgcgc tcgcggatgc cggtcctgcc cgccttgcat tcggagacgt
     4261 agcgcgtcac cggcggctcc cagaagcgct cgttgctggt gttgatcgcg aattcgctcg
     4321 tgtcctccgg aatgcgcagg ttcgagtgcg tgaggatgaa gttgccgatc tcgcggtcga
     4381 gcgtgaagcc gtgcgtgccc ttgcccacgg tcagcaccag catcgtcgcc gggccgtaga
     4441 tcgcgcagcc ggcggcgacc tgctcggtgc ccttctgcag gtagtccttg tcggtgggcg
     4501 cggcggagcc ggcgtgcctg agcaccgaga agatcgtgcc gaccgaaacg ttgacgtcga
     4561 tgttcgacga gccgtcgagc ggatcgaaga ccagcaggta cggcccgcgc ggatactcgt
     4621 ccggcagcgc gtatggcgta tcgagttcct cggacgccat gccggcaacg agcccgcccc
     4681 actcgcagtg gcgcagcatc gtctcgttcg attgcatgtc gagcggctgc tgttcgtcgc
     4741 cgctggcgtt gcgggcggcc gccgcgccat gagaggcgcc gagcgcgccg cgcgcggtca
     4801 ttgcggagat cgccttgatc gcggcggcga cgtcgaccag caggccggcg agcttgccgt
     4861 ggtcgggcac gtcgtgcagc tgctggatca ggaatttcga aagggtcgta cggccgtgat
     4921 gcatggcgct accttggttg aggggaatcc tgcgaatcga gtccgccggc gaacacgcgc
     4981 gaggcgcgga tgtcgtccgc ggcgatctgc atcagcgcg
//
LOCUS       DBNHBY010000721         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_392047, whole genome shotgun sequence.
ACCESSION   DBNHBY010000721 DBNHBY010000000
VERSION     DBNHBY010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_392047"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1672)
                     /locus_tag="AC16HB_17030"
     CDS             complement(<1..>1672)
                     /locus_tag="AC16HB_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608548.1"
                     /translation="FLLHVRSALVAIVMLPIGVLIAFICMHALGINSNIMSLGGIAIA
                     IGAMVDAAIVMIENAHKHLERLPSGGDRKQAIIDAGKEVGPALFFSLLIITVSFLPVF
                     TLEAQEGRLFAPLAYTKTFAMAGAALLSVTLVPLLMIWLIRGRILPEAKNPVNRVLIA
                     VYRPVIAWVLRNKVATIVLSLVVGAASIWPALRLGTEFMPTLNEGTLFYMPTSLPGMS
                     VTKAGELVQMQNKIIMSFPEVESVIGKAGRAATATDPAPLEMFETVINLKPETQWRPG
                     MNIDKLIAEMDKSLQFPGVANSWTMPIRARIDMLSTGIRTPIGIKVFGSDLAEMERVA
                     REIETVVRNVPGTTSAYAERLTGGYYLDVVPDRAQLARYGLSVGALQDVVATALGGEM
                     VTTTVEGRNRFGVIVRYPRDARADPQRIEREVLVPIMGESGQPPAMLPLGQVARVQLV
                     QGPPQIRTENALLSAYIYVDIRDRDIGGYVHDAQKAVRENINLLPGMYVTWSGQFEYM
                     ERALEKLKIVLPLTIGIIFVLLYLNFRRVTDTLIVMLSVPFALIGGIWL"
BASE COUNT          298 a          566 c          525 g          283 t
ORIGIN      
        1 agccagatgc cgccgatcag cgcgaacggc accgacagca tcacgatgag cgtgtcggtc
       61 acgcggcgga agttcaggta cagcagcacg aagatgatgc cgattgtcag cggcaagacg
      121 atcttcagct tctcgagcgc gcgctccatg tactcgaact ggccgctcca ggtcacgtac
      181 atgccgggca gcaggttgat gttctcgcgc accgccttct gcgcatcgtg cacgtagccg
      241 ccgatgtcgc ggtcgcggat gtcgacgtag atgtacgcgg agagcagtgc gttctcggtg
      301 cggatctgcg gcggcccctg caccagttgc acacgcgcga cctgcccgag cggaagcatc
      361 gccggcggct gacccgattc gcccatgatc ggcaccagca cctcgcgctc gatccgctgc
      421 gggtcggcgc gcgcatcgcg cggatagcgg acgatcacgc cgaagcggtt gcgcccttcg
      481 acggtagtcg tgaccatctc gccgccgagc gcggtggcga ccacgtcctg cagcgcgccg
      541 acgctcaggc catagcgcgc gagctgcgcg cggtcgggca cgacgtcgag gtagtaaccg
      601 ccggtcagcc gctccgcgta ggcactcgtg gttcccggaa cgttgcgcac cacggtctcg
      661 atctcgcgcg ccacgcgttc catctctgcg agatccgagc cgaacacctt gatgccgatc
      721 ggcgtgcgga taccggtgga cagcatgtcg atgcgcgcgc ggatcggcat ggtccaggag
      781 ttggccacgc cgggaaactg cagggacttg tccatctcgg cgatcagctt gtcgatgttc
      841 atgccgggac gccattgcgt ttcgggtttc aggttgatca ccgtctcgaa catctccagc
      901 ggcgccggat cggtcgcggt cgccgcgcgg cccgccttgc cgatcaccga ttccacttcg
      961 ggaaagctca tgatgatctt gttctgcatc tgcaccagct cgccggcctt ggtcaccgac
     1021 atgccgggca gcgaggtcgg catgtagaac aacgtgccct cgttcagcgt cggcatgaac
     1081 tcggtcccca gcctcaatgc cggccagatc gacgccgcac cgacgacgag agaaagcacg
     1141 atcgtcgcca ccttgttccg cagcacccac gcgatcaccg gccggtacac cgcaatgagc
     1201 acgcggttca ccgggttctt cgcctccggc aggatgcggc cgcggatcag ccagatcatc
     1261 agcagcggca ccagcgtgac gctcaacaac gccgcgcccg ccatcgcgaa ggtcttggtg
     1321 taggcgagcg gcgcgaacag ccgaccttcc tgcgcctcga gcgtgaacac cggcaggaag
     1381 ctcacggtga tgatcagcag cgaaaagaag agcgccggcc cgacctcttt gccggcgtcg
     1441 atgatcgcct gcttgcggtc gcctccgctc ggcaatcgtt cgaggtgctt gtgcgcgttc
     1501 tcgatcatca cgatcgcggc gtcgaccatc gcaccgatcg cgatagcgat cccgcccagg
     1561 ctcatgatgt tcgagttgat gcccagcgcg tgcatgcaga tgaacgcgat cagcacgccg
     1621 atcggcagca tgacgatcgc caccaaggcg ctcctcacgt gcagcaggaa ca
//
LOCUS       DBNHBY010000722         2141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_933506, whole genome shotgun sequence.
ACCESSION   DBNHBY010000722 DBNHBY010000000
VERSION     DBNHBY010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_933506"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="AC16HB_17035"
     CDS             <1..170
                     /locus_tag="AC16HB_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5608549.1"
                     /translation="IACGISGAIQHLAGMKDSKVIVAINKDPEAPIFSVADYGLEADL
                     FAVVPELVAAL"
     gene            400..642
                     /locus_tag="AC16HB_17040"
     CDS             400..642
                     /locus_tag="AC16HB_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608550.1"
                     /translation="MSDDDLKAELERLRKENAALKKGAATGVTMKVSEKGGLSIYGMG
                     RFPVTLYKEQWLKLLGMADEIRAFIAANDAQLKTKE"
     gene            complement(884..960)
                     /locus_tag="AC16HB_17045"
     tRNA            complement(884..960)
                     /locus_tag="AC16HB_17045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Arg,seq:acg)
     gene            complement(1003..>2141)
                     /locus_tag="AC16HB_17050"
     CDS             complement(1003..>2141)
                     /locus_tag="AC16HB_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608551.1"
                     /translation="REHALADVQLGHDDIAFLQYTGGTTGVAKGAMLTHGNMVANVLQ
                     TNAWCARDLQEGKETAIVPLPLYHVYALTMNLAFLRLGTHTVLITNPRDMPAFIDTLK
                     KTPFTAIIGVNTLYNALLNAPGFGELNLKTMKLTSAGGMAVQRAVAERWKQATGIPIV
                     EGYGLTETAPVVIANALNIKDWTGTIGLPIPSTEAATLDESGNIVAPGQMGEISIRGP
                     QVMKGYWNRPDETAKVFTADGWFRTGDMGIMDEKGYFRITDRKKDMIIVSGFKVFPNE
                     IEDVVTMHPGVLEAAAIGVPDEKSGEAVKLIVVPKDPALKEADLLAHCREHLTGYKVP
                     KQIELRTEPLPKSNIGKILRRLLRESTTGPTTAAQPAKEVATAS"
BASE COUNT          380 a          664 c          692 g          405 t
ORIGIN      
        1 acatcgcgtg cggtatcagc ggagcaatcc aacaccttgc aggcatgaag gacagcaagg
       61 tgatcgtcgc gatcaacaag gaccccgagg cgccgatctt cagcgtggct gactacgggc
      121 tcgaagcgga tctttttgca gtggtgccgg agctggtcgc cgcgctctga gcgcgaggga
      181 accgagcatt tcgcgcgaca ccgcgtacgc ggcctcgcgc accgataccg acctgcgagt
      241 gcagccgcta agcgcccgta cgctcgatac tctgatcgtc gaggcgccgt cgctggcccc
      301 cgcggctcgc ctgcggaccg aagtgctgcg cgaaatgcgc ggtgcacccc gatacactcg
      361 tcgggctgct ggctgatttc caaatgatcg ggagtaacga tgtccgacga tgatctgaag
      421 gccgaactgg aacggctgcg caaggagaac gctgcgctca agaagggggc tgccaccggc
      481 gtcaccatga aagtcagcga aaagggcggg ctttcgatct atgggatggg gcgtttcccg
      541 gtgacgcttt acaaggagca gtggctcaag ctgctcggta tggccgatga gattcgagca
      601 ttcattgcgg cgaacgatgc gcagttgaaa acgaaggagt gagggcttcg agcaggccca
      661 tttgacctgg gcgagcgacg ctgccagcag tcaaaatctg cggtccctgg ccactggtcc
      721 ggcaccgtgc agatgtcaga cgcgggcagt tgcatggtca tctccttggg atttctcccg
      781 ccatgagatt gaaacgagcg attcaagccg aaggcaaagc gtagggccgg cgtagaaccg
      841 caaaaaagaa aaggggctgc atcactgcaa ccccttgaat agttggcgcg cccggcagga
      901 ttcgaaccca cgaccccttg gttcgtagcc aagtactcta tccaactgag ctacgggcgc
      961 gaggcggcga agtataccgg agcctcgcac ccgtccgtcg aatcagctcg cggtcgcgac
     1021 ctccttggcg ggctgagccg cggtggtcgg ccccgtcgtc gactcgcgca gcagccgccg
     1081 caggatcttg ccgatgttcg atttcggcag cggctcggtg cgcagctcga tctgcttcgg
     1141 caccttgtag ccggtcaggt gctcacggca gtgcgcgagc aggtcggcct ccttcagcgc
     1201 cggatccttc ggcacgacga tgagcttgac ggcctcgccg gacttctcgt ccggcacgcc
     1261 gatcgccgcg gcttcgagta cgccggggtg catcgtgacc acatcctcga tctcgttcgg
     1321 gaacaccttg aagcccgaga cgatgatcat gtccttcttg cgatcggtga tgcggaagta
     1381 gcccttctcg tccatgatcc ccatgtcgcc ggtgcggaac cagccgtcgg cggtgaagac
     1441 cttggcggtt tcgtcgggcc ggttccagta gcccttcatc acctgcggac cgcggatgct
     1501 gatctcgccc atctgtccgg gggcgacgat gttgccgctt tcgtccagcg tggcggcctc
     1561 ggtcgacgga atcggcaggc cgatcgtgcc ggtccagtcc ttgatgttca gcgcattggc
     1621 gatcacgacc ggcgcggttt cggtcagccc gtaaccctcg acgatcggaa ttccggtggc
     1681 ctgcttccag cgctccgcga ccgcgcgctg caccgccatg ccaccggccg acgtgagctt
     1741 catcgtcttc aggttcaact cgccgaagcc cggcgcgttg agcagcgcgt tgtacagcgt
     1801 gttgacgccg atgatggccg tgaacggcgt cttcttcagc gtatcgatga acgcgggcat
     1861 gtcgcgcggg ttggtgatca gcaccgtgtg cgtgccgaga cgcaagaacg cgaggttcat
     1921 ggtcagcgcg tagacgtgat agagcggcag cggaacgatt gcggtttcct tgccctcctg
     1981 caggtcgcgc gcgcaccagg cgttggtctg caatacgttc gcgaccatgt tgccgtgagt
     2041 cagcatcgca cccttggcca cgcccgtcgt gccgcccgtg tactgcagga acgcgatgtc
     2101 gtcgtggccg agttgaacgt ccgccagcgc gtgttcgcga c
//
LOCUS       DBNHBY010000723         3365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1609446, whole genome shotgun sequence.
ACCESSION   DBNHBY010000723 DBNHBY010000000
VERSION     DBNHBY010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1609446"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="AC16HB_17055"
     CDS             complement(<1..1604)
                     /locus_tag="AC16HB_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5608552.1"
                     /translation="MHVTTPPVTFLFTDIEGSTRLWEQEPQRMRSALARHDAVSRAAV
                     ARHRGRLVKSTGDGLHAAFDDPLDALAAALEMQLAFAGPEATSDLQLNVRCGMHLGTS
                     ERRDDDYYGPTVNRAARLMGAAYGGQILLSQAVADGVRDRLPAEVSLRDLGTVRLRDL
                     SAELVFQAVHARLRADFPALQSLASTPNNLPQQLNSFIGRERELAQVRQLLAENRLVT
                     LLGVGGIGKSRLSVQLGAAVLGDFPDGVWLIELAPLADPRMVAPTVAAVLGVRESAGH
                     SVADALEEFVRARRLLLILDNCEHVLRASADLAKRLLQAGAGVKALATSRDFLRIAGE
                     AAFHMPTLATPDPRQPIVPETLTQHESVQLFVDRTVAAQPSFRLGADNAAHVAEICRR
                     LDGIPLALELAAARARSLSIAAIAARLDDRFRLLVSRDQTALPRQRTLRALIDWSHDL
                     LDEEEQWLFARLSVFAGGFTLEAADRVGASAARAVDVLDLLTGLVEKSLVAMEADGAR
                     YRMLETVREYAREKLDLADDRDSAHDR"
     gene            1798..2505
                     /locus_tag="AC16HB_17060"
     CDS             1798..2505
                     /locus_tag="AC16HB_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5608553.1"
                     /translation="MNAIDPAAPTATASDAALADLAREGHAAAFELIMRRHNRLLFRA
                     VRGVLDSDADAQDAVQEAWLNAFQRLATFRGEARLATWLTRIALNVALDLRRGRRAAP
                     TEPLPDHESQCADEPLMPFSIPAHEAPEPAAQRAEMRTLLERAIGRLPPLYRSVFMLR
                     AVQEMSVEDTARALHVSADVVKTRFLRARALLRDAIGAQIEGEIPQAFAFDGARCDAV
                     VAHVLAELARRTPLEPG"
     gene            2557..3144
                     /locus_tag="AC16HB_17065"
     CDS             2557..3144
                     /locus_tag="AC16HB_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR5608554.1"
                     /translation="MRTQFSVPSHALSLARRRALTTTGFTLSAAAVALLGGRDALAAT
                     KGDSRDHAGDVRILNTALGAEYEAVAAYQVGADSGLLKGDALTLAQQFQGHHKAHAEA
                     IEKTVTKLGGRAVEELKDYRFPVEKLKTQADVLRFAAGLEKGAVSAYLGAVPLFGDRE
                     LAKVAASILGDEAMHWAVLRHALGETPVVPVSFVA"
BASE COUNT          512 a         1204 c         1185 g          464 t
ORIGIN      
        1 cggtcatgcg ccgagtcgcg atcgtccgcg aggtcgagct tttcgcgtgc gtactcgcgt
       61 acggtctcca gcatccggta gcgtgcgccg tccgcttcca tcgcgaccag cgatttctcc
      121 accaggcccg tcagcaggtc cagcacgtcg acggcgcgcg cggcgctcgc gccgacccgg
      181 tcggccgctt ccagtgtgaa gccgccggca aataccgaca ggcgcgcgaa cagccattgt
      241 tcctcctcgt cgagcaggtc gtggctccag tcgatcagcg cgcgcagcgt gcgctggcgc
      301 ggcagcgcgg tctggtcgcg gctgacgagc aggcgaaagc ggtcgtccag ccgcgcggca
      361 atcgcggcga tcgacagcga gcgcgcgcgc gccgccgcga gttcgagcgc gagcggaatg
      421 ccgtcgagcc ggcggcagat ctccgccacg tgggcggcgt tgtccgcgcc gaggcgaaac
      481 gagggctgtg cggcgacggt gcgatcgacg aagagctgca ccgactcatg ctgcgtcagc
      541 gtctccggaa cgatcggctg gcgcggatcg ggcgtggcca gggtcggcat gtgaaaggcc
      601 gcctcgccgg cgatgcgcag gaagtcgcgg ctggtcgcga gcgccttgac gcccgcgccg
      661 gcctgcagca gccgcttggc gagatcggcg ctcgcccgca gcacgtgctc gcagttgtcg
      721 aggatcagca gcagtctgcg cgcgcgaacg aactcctcca gcgcatcggc gacggaatgg
      781 cccgccgact ccctgacgcc gagcaccgcc gccaccgtcg gcgcgaccat ccgcgggtcg
      841 gcgagcgggg ccagctcgat cagccacacg ccgtcaggga aatcgccgag caccgcggcg
      901 ccgagttgca ccgacaggcg cgacttgccg atgccgccga cgccgagcag cgtgaccagc
      961 cggttctccg cgagcaattg ccggacctgg gcgagctcgc gctcacgacc gatgaacgag
     1021 ttcagttgct gcggcaggtt gttcggcgtg ctggcgagcg actgcagcgc ggggaaatcc
     1081 gcgcgcagcc gcgcatgcac ggcctggaat accagctccg ccgacagatc ccgcagtcgc
     1141 acggtgccga ggtcgcgcag cgaaacctcg gccggcaggc gatcgcgcac gccgtcggcc
     1201 accgcctgcg acagcagtat ctgtccgccg taggccgcac ccatcaggcg cgcggcgcga
     1261 ttgaccgtcg ggccgtagta gtcgtcgtcg cggcgctcgc tcgtgcccag gtgcatgccg
     1321 caacgcacgt tcaactgcag gtcggaggtc gcctccggcc cggcgaacgc aagctgcatt
     1381 tccagcgcgg ccgcgagggc gtcgagcgga tcgtcgaatg ccgcgtgcag gccgtcgccc
     1441 gtcgacttga ccagcctgcc gcgatggcgc gcgaccgccg cacgcgacac cgcgtcgtgg
     1501 cgcgcgagcg cgctgcgcat ccgctgcggc tcctgctccc acaggcgcgt actgccctcg
     1561 atgtcggtga acaggaacgt gactggcggg gtcgtcacgt gcatggcgtt ccgggtggca
     1621 ttgcagtcgc ccgcaaacgt agcacgggca tcggacggcg ggacccgcgc gcagaagtta
     1681 cgcttcgcac cgtaccgaag ccaacatgca tggcgcgatt ggccgcatgc gcgcaagggt
     1741 cgcgcatgtc accttttgcc gtccgctgcc gtctaacgac agacccgcga gaacgacatg
     1801 aacgccatcg accccgccgc accgaccgca accgcctccg acgccgcgct ggccgatctg
     1861 gcgcgcgaag gtcacgccgc agcgttcgag ctcatcatgc ggcgccataa ccggctgctg
     1921 ttccgcgcag tgcgcggcgt gctcgacagc gatgccgacg cgcaggatgc cgtgcaggag
     1981 gcgtggctca atgcgttcca gcgcctcgcc acgttccgcg gcgaggcgcg gctggcgacg
     2041 tggctcacgc gcatcgcgct gaacgtggcg ctcgacctgc gccgcggccg ccgcgcggcg
     2101 ccgaccgagc cgctgcccga tcacgaatcg caatgcgccg acgagccgct gatgccattt
     2161 tccatccctg cacacgaagc gccggaaccg gctgcgcagc gcgcagaaat gcgcacgttg
     2221 ctcgagcgcg cgatcggccg cctgccgccg ctctatcgca gcgtattcat gctgcgcgcc
     2281 gtgcaggaaa tgtcggtcga ggacaccgcg cgcgcgctcc acgtcagcgc cgacgtggtc
     2341 aagacgcgct tcctgcgtgc gcgcgcgttg ttgcgcgacg ccatcggcgc gcagatcgaa
     2401 ggcgagattc cgcaggcatt cgcgttcgac ggcgcgcgct gcgacgccgt tgtcgcacac
     2461 gtactggccg agctcgcgcg gcgcacgccg ctcgagcccg gctagcggcg gcggcgtccg
     2521 ccgcccgccg ttcccccacc tctccaagga gctgaaatga gaacgcaatt cagcgtgcct
     2581 tcgcacgccc tgtcactggc gcgccggcgc gcgctgacga ccaccggatt cacgctgtcg
     2641 gccgccgcgg tcgcgctgct cggcggccgc gacgcgctcg cggccacgaa gggcgacagc
     2701 cgcgatcacg ccggtgacgt gcgcatcctg aacaccgcgc tcggcgccga gtacgaggcg
     2761 gtcgctgcct accaggtcgg cgccgacagc ggactgctca agggcgatgc gctgacgctg
     2821 gcgcagcagt tccagggcca tcacaaggcg cacgccgagg cgatcgagaa gacggtgacg
     2881 aagctcggcg gccgcgcggt cgaggaactc aaggactaca ggttccccgt cgagaagctc
     2941 aagacacagg ccgacgtgct gcgcttcgcg gctgggctgg aaaagggcgc ggtgtccgcg
     3001 tacctgggcg cggtaccgct cttcggcgat cgcgagctgg ccaaggtcgc cgccagcatt
     3061 ctcggcgacg aggcgatgca ctgggccgtg ctgcggcacg cgctcggcga aacgcccgtg
     3121 gtgccggtgt cgttcgtcgc atgagcgccg cgcggcagat cggaaggcgc tcggccttgc
     3181 aggccgcggc gttgcgagac gcgccgcctc ctcggggaga gagccagggg gcaacatgag
     3241 cgcggcccgc gcgctggctg cgctgcttgc cgccgtcagt gccggcgcgt gggcagcaac
     3301 cgcagcgcag ctttcgcacg gcgaggagat ctattcgcgc tgtgccgcgt gtcactcgat
     3361 cgaag
//
LOCUS       DBNHBY010000724         2971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_257380, whole genome shotgun sequence.
ACCESSION   DBNHBY010000724 DBNHBY010000000
VERSION     DBNHBY010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_257380"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16HB_17070"
     CDS             complement(<1..380)
                     /locus_tag="AC16HB_17070"
                     /inference="COORDINATES: protein motif:HMM:NF016705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOUL family heme-binding protein"
                     /protein_id="HIR5608555.1"
                     /translation="MHALTAAAIAAVVTVIATPAAMAIEEPRFNVLEKDGSFELREYA
                     PYVVAETRVEAGFEEAGSVAFQRLFRYISGDNVARQKIAMTAPVTQARGEKISMTAPV
                     SQVADGNAYLVAFTLPSTYTLATAP"
     gene            complement(480..1712)
                     /locus_tag="AC16HB_17075"
     CDS             complement(480..1712)
                     /locus_tag="AC16HB_17075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5608556.1"
                     /translation="MTRVAVTGIGVVSPLGNSAAEVIDRACSGRAAIHRLEGPFARRL
                     AGPLVASAQFDGAEHFDPPRLRMLDRVSHFACFAARQALAESNIDLAQADRRRIGVFL
                     GTGMGGSQSSDDGYRSLYEDGSDRVKPFTVLLGMHNAPAAWIGIEHDLRGPNLTYSTA
                     CSSSAVAIGEAALRIARGDLDLALAGGAEAPLSFGSLKAWEALHTLASVDAEDASASC
                     KPFARDRSGMVLGEGSAMVLLEAWEHAVARGATILGEIAGYGFANDAGHITRPSLEGQ
                     AAAIEAALVAAAVDPDAVDAINAHGTGTQANDGVETAAIKAVFGDRAARIPISATKAM
                     HGHLLGAAGALECVLSLLAMQRQVALPTMHLHVPDPECDLDYVPNQVRQGVAARTMIS
                     NSFAFGGANAVLVLRGAP"
     gene            complement(1709..1999)
                     /locus_tag="AC16HB_17080"
     CDS             complement(1709..1999)
                     /locus_tag="AC16HB_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5608557.1"
                     /translation="MDTTIDRLRQLLAKDYKLDPGALVPDAPLEALGIDSLGVAELMF
                     NIEDEFKVTIPEEPVALKTIGDVVTYIQGLVDKQQAAAPAGNAAALRAASGR"
     gene            complement(2001..>2971)
                     /locus_tag="AC16HB_17085"
     CDS             complement(2001..>2971)
                     /locus_tag="AC16HB_17085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5608558.1"
                     /translation="MSGERTIEADLPPPAAGTQQWGRALGRETLDVLLLRQAVAMGVQ
                     VLQPWSVKAIDGAPGDWHCRMNSTQTDESTILRARVAIDAHGSWQALPNARRRHRIPS
                     RGSDLLAFKANFRNADLVDGLLPVLAFDGGYGGMVVADGGLTTVACCIRRDRLEACRT
                     AAPGLRAGEAVEAYLRAQCRGVREALQPATRVGPWLAAGPLQPGIRVDGHDGPYRIGN
                     AAGEAHPIIGEGMSMALQSAWLLTDHLLTSGKPGGGAKSRQRDVARGYAAEWRRQFVP
                     RLLFAAGFARMAMSPVTTAPLVGLAQAWPGMLTFGARIKTPLTQRT"
BASE COUNT          461 a         1021 c         1023 g          466 t
ORIGIN      
        1 ggcgccgtgg ccagcgtgta ggttgacggg agagtgaagg cgacgaggta ggcgttgccg
       61 tcggccacct gcgacaccgg cgccgtcatg ctgatctttt ccccgcgtgc ttgagtgaca
      121 ggtgccgtca tcgcaatctt ctgacgcgcg acgttgtcgc cactgatgta gcggaacaga
      181 cgctggaacg cgacgctacc cgcctcctcg aagccggctt cgacacgggt ctccgcgacg
      241 acatacggcg cgtactcgcg cagttcgaag ctgccgtcct tctcgagtac gttgaagcgc
      301 ggttcttcaa tggccatggc ggcgggcgtc gcgatcacgg tgacgacggc cgcgatggcc
      361 gcggcggtca gcgcatgcac gatgacgtcc tcagtgcggc acggattgtc cggcttccag
      421 tgtacgccgc gccttccagc agcagcggat aatgagacgg gcgagctcgt cgcgggcgct
      481 cacggcgccc cgcgcagcac cagcaccgca ttcgcgccgc caaaggcgaa cgagttcgag
      541 atcatcgtcc gtgcggcgac cccctggcgc acctgattgg gtacgtagtc gaggtcgcac
      601 tccgggtcag gcacgtgcag gtgcatcgtc ggcaacgcga cctggcgctg catcgccagc
      661 agcgacagca cgcactctag cgcccccgcc gcgcccagca ggtggccgtg catggccttg
      721 gtcgcgctga tcgggatgcg ggccgcgcga tcgccaaaga cggccttgat cgcggcggtt
      781 tccacgccat cgttggcctg ggtgcccgtg ccatgggcat tgatcgcgtc gaccgcatcc
      841 ggatcgacgg cggccgcgac cagggcggcc tcgatggccg cggcctggcc ctcgagactc
      901 ggtcgcgtaa tgtggcccgc gtcgtttgcg aagccatagc cggcgatctc gcccaggatg
      961 gtcgcgccgc gcgccacggc gtgctcccag gcttcgagca ggaccatggc cgagccctcg
     1021 ccgagcacca tgccgctgcg gtcccgcgca aagggcttgc acgaggccga cgcatcttcc
     1081 gcatcgacgc ttgcgagggt gtgcagcgct tcccaagcct tgagtgagcc gaacgagagc
     1141 ggtgcctcgg cgccgcccgc gagcgcgagg tcgaggtccc cgcgtgcgat gcgcagcgca
     1201 gcctcgccga tcgcgacggc cgacgacgag cacgccgtcg agtacgtcag gttgggtccg
     1261 cgcaggtcgt gctcgatgcc gatccaggct gcgggcgcat tgtgcatgcc gagcagcacg
     1321 gtgaacggct tgactctatc cgagccgtcc tcgtacagcg acctgtaccc gtcgtcgctc
     1381 gattgcgatc cgcccatgcc ggtgccgagg aagacaccga tgcggcggcg gtcggcctgc
     1441 gccaggtcga tgttcgattc ggccagggcc tgccgtgcgg caaagcacgc aaagtggctc
     1501 acgcgatcga gcatgcgcag gcggggcgga tcgaaatgtt ccgcgccgtc gaactgtgcc
     1561 gaggccacga gcgggccggc cagccggcgg gcgaagggtc cttcgagccg atggatggcc
     1621 gcacggcctg agcaggccct gtcgatgacc tcggccgcgc tgttgccgag cggcgagacg
     1681 acgccgatac cggtgacggc gacgcgtgtc atcgccccga cgccgcgcgc aaggccgcgg
     1741 cgttgcctgc aggtgcggct gcctgttgct tgtcgacgag accctggatg taggtgacga
     1801 cgtcaccgat cgtcttcagc gcgaccggtt cctccgggat ggtgaccttg aactcgtctt
     1861 cgatgttgaa catcaactcg gccacgccga gcgagtcgat gcccagtgct tccagcggcg
     1921 cgtccggcac cagagcgccc ggatcgagct tgtagtcctt ggccagcaac tgtcgcagtc
     1981 gatcgatcgt ggtatccatg tcacgtcctc tgtgtcaggg gtgtcttgat gcgtgcgccg
     2041 aaagtcagca ttccgggcca ggcctgcgcg aggcccacca gtggcgcagt ggtgacgggc
     2101 gacatcgcca tgcgcgcaaa gccagccgcg aagagcagtc gcggcacgaa ctgccggcgc
     2161 cactccgccg cgtacccgcg ggcgacgtcg cgttgtcgcg actttgcgcc gccgcccggc
     2221 ttgccggacg tgagcaggtg gtccgtcagc agccacgccg actgcagtgc catgctcatg
     2281 ccttcgccga tgatcgggtg cgcctcaccg gccgcgttcc cgatgcggta cggcccgtcg
     2341 tggccgtcga cgcggatgcc gggttgcagc ggcccggccg cgagccaggg accgacacgc
     2401 gtcgcaggtt gcagggcctc gcgcacgccg cggcactgcg cccgcaaata ggcctccacg
     2461 gcctcaccag cacgcagacc cggtgcggcg gtacggcagg cttccaaccg gtcgcgacga
     2521 atgcagcagg ccacggtggt caggccacca tcggcgacga ccatcccgcc atagcccccg
     2581 tcaaaggcga gcacgggcag caggccgtcg acgaggtctg cattgcgaaa gttggccttg
     2641 aaggcgagca ggtccgatcc cctcgatggg atacggtgac gtcggcgcgc attcggcagc
     2701 gcctgccacg agccgtgggc gtcgatggca acgcgcgccc gcaggatcgt cgattcatcg
     2761 gtctgcgtgg aattcatccg gcagtgccag tcgccgggtg cgccgtcgat ggccttcact
     2821 gaccagggct gcagaacctg aacacccatg gcaaccgcct gacggagcag cagtacgtcg
     2881 agcgtctcgc gaccgagcgc acgcccccac tgctgcgtcc ctgccgcggg cggcgggagg
     2941 tccgcttcga tggtccgctc gccgctcatc a
//
LOCUS       DBNHBY010000725         3814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_324464, whole genome shotgun sequence.
ACCESSION   DBNHBY010000725 DBNHBY010000000
VERSION     DBNHBY010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_324464"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16HB_17090"
     CDS             complement(<1..243)
                     /locus_tag="AC16HB_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5608559.1"
                     /translation="MKTEAVAPVRKSLRVNLAPAQAFELFTRDLARWWPLATHSCSGD
                     AKASVVFEGVGGKVVETAGDGTRPVWGALLDWNPPHS"
     gene            complement(240..575)
                     /locus_tag="AC16HB_17095"
     CDS             complement(240..575)
                     /locus_tag="AC16HB_17095"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5608560.1"
                     /translation="MAKSSSAVLAALADPTREQIVRLLLRGEKSVGEIAARLPVSRPA
                     VSKHLRLLESAGLVTFRSFATRNVYAVEPAALQSLRDELNRMWEQALARYALAADNRG
                     RKSARRDNG"
     gene            complement(668..1645)
                     /locus_tag="AC16HB_17100"
     CDS             complement(668..1645)
                     /locus_tag="AC16HB_17100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR5608561.1"
                     /translation="MKAIQCVEWGPPDRLRLVDVADPQPKPGEVRVRVRAAGVNYPDA
                     LIVQKKYQVQPPLPFTPGAEVAGEIDAVGEGVTDRTIGQPVVAFVGLGAFAEKVCTDV
                     RRTIPLPSGIGEDIAAGFTLTYATSQHALAERAQLKAGETLLVLGAGGGVGLAAVELG
                     KLAGARVIAAAGTDAKLAACRAAGADALVNYSTHDLRDAVKSLTEGKGVDVIYDPVGG
                     AYTETALRLIAWRGRLLVIGFAGGEIPRIPANLLLLKEASAVGVYWGEFAQRDPKGNA
                     RLIGELMTWLAQGRLKPRVPQAFALADTARALEDLLQRRAIGKLVVRPG"
     gene            1724..2965
                     /locus_tag="AC16HB_17105"
     CDS             1724..2965
                     /locus_tag="AC16HB_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5608562.1"
                     /translation="MNFDFAPKVNDLRARVDAFMQEHVFPNERRFYDEVESNTRAGRR
                     WTPTTLVEELKPKARAAGLWNLFLPASEHGAGLTNLEYAPLAELMGRVPWASEVFNCS
                     APDTGNMETIERYGTPEQKRQWLEPLLRGEIRSAFAMTEPAVASSDATNIQTSMKRTG
                     DHYVINGRKWWTSGAGDPRCQIYIVMGKTNPDAPKHAQQSMILVPADTPGLKVLRPLN
                     VFGYDDAPHGHMEVDFSDVRVPASNVLLGEGRGFEIAQGRLGPGRIHHCMRCIGLAER
                     ALQLMCTRLASRVAFGSPVAKQTVWQERIAEARCMIEQARLLTLKAAYMMDTVGNKAA
                     KAEIAMIKVVAPNVACTVADWAIQAHGGGGMCDDFPLAYAYSLARTLRLADGPDEVHR
                     NAIAKMEIARHAPIPRKEGTS"
     gene            2962..3654
                     /locus_tag="AC16HB_17110"
     CDS             2962..3654
                     /locus_tag="AC16HB_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HIR5608563.1"
                     /translation="MIDVYTWPTPNGHKVHIMLEECGLPYRVHAVDIGAGDQFSIEFL
                     EVSPNNKIPAIVDHSGPDGKALSLFESGAILLYLAGKTGRLLPADVRGKYVALQWLMF
                     QMGSVGPMLGQAHHFRLYAPEKIEYAINRYTSEAKRLYNVMDRRLREVPFFAGDEFTI
                     ADIAIFPWTRSHANQGVDLAEFPHFKRWFDTVAARPTVQRGLQVLAERRKPITTEQAR
                     RNLFGDKQYERR"
BASE COUNT          653 a         1334 c         1255 g          572 t
ORIGIN      
        1 cgaatggggc ggattccagt cgagcagcgc accccacacg gggcgcgtgc cgtcgcctgc
       61 agtctcgacg accttgccgc cgacgccttc gaacacgacc gacgccttcg catccccgga
      121 gcacgagtgc gtggcgagcg gccaccagcg tgccagatcg cgcgtaaaga gctcgaacgc
      181 ctgcgcgggg gccaggttca cccgcagcga cttgcggacc ggagcgactg cttcagtctt
      241 catccgttgt cgcgccgcgc ggatttcctg ccgcggttgt cggccgccag agcgtaccgt
      301 gcaagcgcct gttcccacat gcggttcagc tcgtcgcgca aggactgcaa cgccgccggc
      361 tcgacagcgt aaacgttgcg cgtcgcaaac gaacggaacg tcaccagccc cgcactttcg
      421 agcaggcgca ggtgcttgga caccgccgga cggctgacgg gcagccgtgc ggcgatctcg
      481 ccgaccgact tctcgccccg cagcagcagc cgcacgatct gctcgcgggt cggatccgcc
      541 agcgccgcca gcactgcgct actcgatttc gccattgccc tcaggccgct cggcccgccg
      601 tgttggtaac cacgcggaaa ccgtaactta caggttacta agaatcaagt cccgtcccgc
      661 gcgcgggcta tcccggccgc accaccagct tgccgatcgc gcgccgctgc agcaggtctt
      721 cgagcgcgcg cgcggtgtcg gcgagcgcaa aggcctgcgg cacccgcggc ttgagtcggc
      781 cctgcgccag ccacgtcatc agttcgccga tcaggcgcgc atttcctttg ggatcgcgct
      841 gcgcgaactc gccccagtac acgcccaccg cggaggcttc cttcagcagc agcaggttgg
      901 cggggatcct cggaatctcg ccgccggcga aaccgatcac gagcagccgg ccgcgccacg
      961 cgatcagacg cagcgcggtt tcggtgtatg caccgccgac cgggtcatag atcacatcga
     1021 cccccttgcc ctcggtcagc gacttcacgg catcgcgcag gtcgtgcgtc gagtagttga
     1081 cgagtgcgtc cgcgccggcc gcccggcagg cggcgagctt cgcgtcggtg ccggcggccg
     1141 cgatcacgcg cgcgccggcg agcttgccga gttcgacggc agcgagccct acgccgccgc
     1201 cagcgccaag caccagcagc gtctcgcccg ccttcagttg cgcgcgctcg gccagcgcgt
     1261 gctgcgacgt ggcgtaggtc agagtgaagc cggcggcgat gtcttcgccg atgccagacg
     1321 gcagcggtat cgtgcgccgc acgtcggtgc agaccttctc cgcaaaggcg cccagtccca
     1381 cgaacgcgac tacgggctga ccgatcgtgc gatcggtcac gccctcgccg accgcatcga
     1441 tctcgcccgc gacctcggcg cccggcgtga acggcaacgg cggctggacc tggtacttct
     1501 tctgcacgat cagcgcgtcg gggtagttga cgccggccgc tctgacgcgc acgcgcacct
     1561 cgcccggctt cggctgcggg tcggccacgt cgaccagccg cagacgatcg ggcggccccc
     1621 attcgacgca ctggatcgcc ttcattgcac cctccctttt gatccgaacc agacccgcac
     1681 gcacggtcac ctgcgctatt ctgcctcgcc acaggaggcc gcaatgaatt tcgacttcgc
     1741 gcccaaggtc aacgacctgc gtgcccgcgt ggacgcgttc atgcaggagc acgtcttccc
     1801 caacgagcgc cgcttctatg acgaggtcga atccaatacc cgcgccggca ggcgctggac
     1861 gcccacgacg ctcgtcgagg agctgaagcc gaaggcgcgc gccgccgggc tctggaacct
     1921 gttcctgccc gcatcggagc atggcgccgg gctgacgaac ctcgaatacg cgccactggc
     1981 ggaactgatg ggccgtgtgc cgtgggcgag cgaagtcttc aactgcagcg cgcccgacac
     2041 cggcaacatg gagacgatcg aacgctatgg cacaccggag cagaagaggc aatggctgga
     2101 gccgctgctg cgcggtgaga tccgctccgc gtttgcgatg accgaaccgg cggtcgcgtc
     2161 gagcgacgcc acgaacatcc agacgtcgat gaagcgcacg ggcgatcact acgtgatcaa
     2221 cggccgcaag tggtggacct cgggcgccgg cgatccgcgt tgccagatct acatcgtgat
     2281 gggcaagacg aacccggacg caccgaagca cgcgcagcaa tcgatgatcc tcgtccccgc
     2341 cgacacgccg ggcctgaagg tgctgcgacc gctgaacgtg ttcggctacg acgatgcgcc
     2401 acacggtcac atggaagtcg acttcagcga cgtgcgcgtg ccggccagca acgtcctgct
     2461 cggcgaaggg cgcggtttcg agattgcgca gggtcggctc ggccccggcc gcattcacca
     2521 ctgcatgcgg tgcatcgggc tcgccgagcg cgcgctgcag ctgatgtgca cgcggctcgc
     2581 gtcgcgcgtg gcgttcggct ccccggtggc gaagcagacg gtgtggcagg agcggatcgc
     2641 ggaggcgcgc tgcatgatcg agcaggcgcg gctcctgacg ctgaaggccg cctacatgat
     2701 ggacacggtg ggcaacaagg cggcgaaggc ggagatcgcg atgatcaagg tcgtggcgcc
     2761 gaacgtcgcc tgcacggtgg ccgactgggc gatccaggca cacggcggtg gcggcatgtg
     2821 cgacgacttc ccgctcgcct acgcgtactc gctcgcgcgc acgctgcggc tggccgacgg
     2881 acccgacgag gtccaccgca atgcgatcgc caagatggaa atcgcgcgcc atgcgccgat
     2941 accgcgcaag gaaggaacct cgtgatcgac gtctacacgt ggccgacgcc gaacggccat
     3001 aaggtccaca tcatgctcga ggagtgcggc ctgccgtacc gcgtgcacgc ggtcgacatc
     3061 ggcgccggcg atcagttctc gatcgagttc ctcgaggtca gcccgaacaa caagattccg
     3121 gcgatcgtcg atcacagcgg ccccgacggc aaggcgctgt cactgttcga gtcgggcgcg
     3181 atcctgctgt acctcgcggg caagaccggc aggctgctgc cggccgacgt gcggggcaag
     3241 tacgtcgcgc tgcaatggct gatgttccag atgggcagcg tcggaccgat gctggggcag
     3301 gcgcaccact ttcgcctgta cgcaccggag aagatcgaat acgcgatcaa tcgatacacc
     3361 agcgaggcga agcggctcta caacgtgatg gaccggcgcc tgcgcgaagt tcctttcttc
     3421 gccggcgacg agttcacgat cgccgacatc gcgatcttcc cgtggacgcg ctcgcacgcg
     3481 aaccagggcg ttgatctggc cgaatttccg cacttcaagc gctggttcga cacggtagcc
     3541 gcgcggccga ccgtgcagcg cggcctgcag gtactcgccg agcgacgcaa gccgatcacc
     3601 accgagcagg cacgccggaa cctcttcggc gacaagcaat acgagcgccg gtagcgccga
     3661 tgccgagcac gctcatctcg gtgcgcaagc gctggtcgcc ggagaagcgg cgcgcgatca
     3721 tcgacgccgt acaccaggcg ctggtcgacg gcgtgctggt gccgcagtcc gaccgcacgc
     3781 ttcggctgca ggtgttcgat gcggaggact tcgc
//
LOCUS       DBNHBY010000726         5077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_53954, whole genome shotgun sequence.
ACCESSION   DBNHBY010000726 DBNHBY010000000
VERSION     DBNHBY010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_53954"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="AC16HB_17115"
     CDS             <1..408
                     /locus_tag="AC16HB_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HIR5608564.1"
                     /translation="DRLIAADEAQLLEVPDVGPVVAASIVRFFAEPHNREAIEQLRAV
                     GVTWRESAPRAAPLAGQLAGKTVVLTGTLPTLTREEASELIRAAGGKTASSVSKKTDF
                     VVAGEEAGSKLDKARELGVPVLDEAQLRQMLKG"
     gene            410..1153
                     /locus_tag="AC16HB_17120"
     CDS             410..1153
                     /locus_tag="AC16HB_17120"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR5608565.1"
                     /translation="MYAIIGGSGLAKLSALGNPRRQVLRTPYGEPSGALTFGELAGRK
                     VVFLARHGYGHTIAPHEINYRANIWALKELKVDGVFAVASVGGIGADFGPGTLVVPDQ
                     IIDYSHSRKATFYEGAVAPVTHIDFTWPYAQRIRKLLLDAATRCGEAVADGGVYACTQ
                     GPRLETAAEIERIARDGGDLVGMTGMPEAALAREAGLDYATLAVVVNHAAGRGDSRAA
                     IRLEDLETVLRETIVRAVAVIEAALPEVS"
     gene            1150..1683
                     /gene="def"
                     /locus_tag="AC16HB_17125"
     CDS             1150..1683
                     /gene="def"
                     /locus_tag="AC16HB_17125"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5608566.1"
                     /translation="MIRPILRMGDPRLLEVARPVPGFDTPELHALIADMFDTMHAADG
                     AGLAAPQIGVPLRVVIFGTGERIPRYPSEDPVPRTVLVNPVLTPLSDEMEEGWEGCLS
                     VPGLRGVVARHVKLRYSGFDARGAAIERTVEGFHARVVQHECDHLDGILYPMRVRDFT
                     RFGFTDVLFPDLAPLDE"
     gene            complement(1685..4240)
                     /locus_tag="AC16HB_17130"
     CDS             complement(1685..4240)
                     /locus_tag="AC16HB_17130"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR5608567.1"
                     /translation="MNDVGFALRSELRAERDGLIARFRDGGSVSQLLKGLARAVDRML
                     RRVVEASGITSDVAVIAVGGYGRGELFPHSDVDILLLPRAALDVDGRARIERLIGTLW
                     DTGLHLGHSVRTLEECREEARRDVTVLTAMLESRLIAGPAGIFDDFSRAIISALDSQA
                     YFRAKLLEQQQRHTKYQETPYALEPNCKESPGGLRDLQVMLWIARAAGFGASWRQLAA
                     RGLLTAEEFRLLTRNERTLREIRARLHLVAGRREDRLVFDVQGAVAEAMGIQPSATHR
                     ASERLMQRYYWAAKVVTQLNTILLQNIEERLFPRTDEHLILLDDVFVARNEKLDIVAD
                     DALTRDPNAMLRAFLVLQQHAELKDMSPRLLRALWHGRFLIDAAFRRDPRNRATFIAI
                     LQQPRGIVHGLRRMNQWSVLGRYLPPFRRIIGQMQHDLFHVYTVDQHILQVIRNLRRF
                     TMAEFAHEFPLCSQLIADFERPWLLYIAALFHDIAKGRGGDHSELGARDAITFCRQHG
                     LPREDTALVAFLVEQHLLMSRVAQKQDIADPDVVRRFARQVQTERRLVALYLLTVADV
                     RGTSPRVWNNWKAKLLEDLFLLTRRLLGGDAAPATAELERRKRDALDILRLYGLSEAA
                     HEPLWRQLDVVYFLRHSARDIAWHARTLVAHVGAERAIVRARLAQTGEGAEVLIYVRD
                     QKDLFARVCGYFDSRNLSILDAKIHTTRHGYALDTFLVSDNGRARHYRDLLSRIEREL
                     AECIDQVTPLPPPVKGRLSRHSRHFPVMPAVHLQPDADGRHFLLNVTATDRIGLLYAL
                     TRVLARHGINVHTAKINTLGERVEDVFLIDGPELANARGQLQIEQDLLEALAP"
     gene            complement(4251..5075)
                     /gene="map"
                     /locus_tag="AC16HB_17135"
     CDS             complement(4251..5075)
                     /gene="map"
                     /locus_tag="AC16HB_17135"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5608568.1"
                     /translation="MVIKNAADIDGMRVAGRLAGEVLDFITPHVKPGVTTGELDRLCH
                     DLIVNTQGATPAPLNYAPPGYRPYPKSICTSVNHQVCHGIPGDRVLKNGDIVNIDITV
                     IKDGYHGDTSRMFYVGEPSIAARRLCEITYECMWLGIGQVRAGAFLGDIGHAIQTHAE
                     KHGYSVVREFCGHGIGRKFHEEPQVLHYGKPGTLERLAAGMIFTIEPMINAGRREIRE
                     LADGWTIVTKDHSLSAQWEHTVLVTEAGYEILTLSAGAPAPPRFVLASATPALAPA"
BASE COUNT          809 a         1669 c         1786 g          813 t
ORIGIN      
        1 gaccgcctga tcgcggccga cgaggcacag ttgctcgagg tgcccgacgt cggaccggtg
       61 gtggcggcgt cgatcgttcg cttcttcgcc gaaccgcaca atcgcgaggc gatcgaacag
      121 ctccgggcgg taggcgtgac gtggcgggaa agcgcgccgc gggcagcgcc cttggccggg
      181 cagctggcgg gcaagacggt agtgctgacg ggcacgctgc cgacgctgac ccgggaagag
      241 gcgagcgaac tgatccgcgc cgcaggcggg aagaccgctt catcggtgtc gaagaagaca
      301 gacttcgtgg tcgccggcga agaggcggga agcaagctcg acaaggcgcg cgagctgggc
      361 gtgccggtgc tcgacgaagc gcaactgcga cagatgttga aagggtgaca tgtacgcaat
      421 catcggtggc agcggattgg cgaagctgtc ggcgctgggc aatccgcgcc ggcaggtgct
      481 gcgcacgccg tacggcgaac cgtcgggcgc gctgacgttc ggcgagcttg cgggccgcaa
      541 ggtcgtgttc ctggcgcgcc acggctatgg ccacaccatc gcgccgcacg agatcaacta
      601 ccgcgccaac atctgggcgt tgaaggagct gaaggtcgac ggcgtgttcg cggtcgcatc
      661 cgtgggcggg atcggcgccg atttcggccc cggcacgctg gtcgttccag accagatcat
      721 cgattactcg catagccgca aggccacctt ctacgagggc gccgtcgcac cggtcacgca
      781 catcgatttc acctggccat acgcgcagcg gatcaggaag ctgctgctcg atgccgccac
      841 gcgctgcggc gaggcggttg ccgacggcgg cgtgtacgcc tgcacacaag gaccacgact
      901 ggagaccgcc gccgagatcg aacgcatcgc ccgcgacggt ggcgacctgg tgggcatgac
      961 cggcatgccg gaagccgcgc tggcgcgcga agccggactg gactacgcga cactcgcggt
     1021 ggtggtcaat cacgcagccg gtcgcggcga cagccgcgcg gcgatccggc tcgaggacct
     1081 ggagaccgtg ctgcgcgaga cgatcgtgcg cgcggtggcg gtgatcgaag cggccctgcc
     1141 ggaggtgtcg tgatccggcc catcctgcgc atgggcgatc cgcgcctgct cgaggtggcg
     1201 cggccggtgc ccgggttcga cacccccgaa ctgcacgccc tgatcgccga catgttcgac
     1261 accatgcacg cggccgacgg cgccggtctg gcggcgccgc agatcggcgt gccgctgcgc
     1321 gtggtgatct tcggcaccgg tgagcgcatt ccgcgctatc cgagcgagga cccggtcccg
     1381 cgcacggtgc tggtcaatcc ggtgctgacg ccactgtccg acgagatgga agagggctgg
     1441 gaaggctgcc tgtcggtgcc gggactgcgc ggcgtggtgg cgcgtcacgt gaagctgcgc
     1501 tactcgggct ttgatgcgcg cggtgcggcg atcgaacgca ccgtcgaggg tttccacgcg
     1561 cgcgtggtgc agcacgagtg cgatcatctc gacggcatcc tatatccgat gcgggtgcgc
     1621 gacttcacgc gcttcggctt cacggacgtg ctgtttccgg acctggcgcc gctggacgaa
     1681 tagctcaggg cgccaacgcc tccagcagat cctgttcgat ctgcagctgg ccgcgcgcgt
     1741 tggcgagctc cgggccgtcg atcagaaaca cgtcctcgac gcgctcgccg agcgtgttga
     1801 tcttggcggt gtgcacgttg attccgtgcc gagccagtac gcgcgtgagc gcgtagagca
     1861 gcccgatccg gtcggtggcg gtcacgttga gaaggaagtg gcgtccgtcg gcgtccggct
     1921 gcaggtggac cgccggcatg accgggaaat gccttgaatg gcgcgacagt cggcccttga
     1981 ccggcggcgg cagcggcgtg acctggtcga tgcactcggc cagctcgcgc tcgatgcgcg
     2041 acagcaggtc acgatagtgg cgtgcgcggc cgttgtcgct gaccaggaac gtgtcgagcg
     2101 cgtagccgtg gcgcgtggtg tgaatcttgg cgtcgaggat gctgaggttg cggctgtcga
     2161 aatacccgca tacgcgagca aacaggtcct tctggtcgcg cacgtagatc agcacctccg
     2221 cgccttcgcc ggtctgcgcc aggcgcgcgc gcacgatcgc gcgttcggct ccgacgtgcg
     2281 cgaccagcgt gcgcgcatgc caggcaatgt cgcgcgccga atgccgcagg aagtacacga
     2341 cgtcgagctg ccgccacaga ggttcgtgcg cggcttccga cagcccgtac agcctcagga
     2401 tgtcgagcgc atcgcgcttg cgccgctcca gttccgcggt ggccggcgcc gcgtcgccgc
     2461 cgagcaggcg ccgtgtcagc aggaacaggt cctcaagcag cttcgctttc cagttgttcc
     2521 atacccgcgg gctggtgccg cgcacgtcgg caaccgtcaa caggtacagc gcgaccaggc
     2581 gtcgctcggt ctgcacctga cgcgcgaagc ggcgcacgac gtcgggatcg gcgatgtcct
     2641 gcttctgcgc cacgcgcgac atgagcagat gctgctcgac caggaacgcg accagcgcgg
     2701 tgtcttcccg gggcagacca tgttggcggc agaacgtgat cgcatcgcgc gcgcccagtt
     2761 cgctgtggtc gccgccgcgg cccttggcga tgtcgtggaa gagggctgcg atgtagagga
     2821 gccacgggcg ctcgaagtcg gcgatcagtt ggctgcaaag cggaaactcg tgcgcgaact
     2881 ccgccatcgt gaagcgccgc aggttgcgga tcacctggag gatgtgctgg tcgaccgtgt
     2941 agacatggaa caggtcgtgc tgcatctggc cgatgatgcg gcggaacggc ggcaggtaac
     3001 ggcccagcac cgaccactgg ttcatccgcc gcaacccgtg cacgatgccg cgcggttgct
     3061 gcaggatcgc gatgaaggtc gcccggttgc gcggatcgcg ccggaaggcg gcgtcgatca
     3121 ggaagcggcc gtgccacagc gcgcgcagca ggcgcggcga catgtccttc aactcggcgt
     3181 gctgctgcag cacgaggaat gcgcgcagca ttgcgttcgg atcgcgcgtc agcgcgtcat
     3241 cggcgacgat gtcgagtttc tcgttgcgcg ctacgaacac gtcgtcgagc aggatcagat
     3301 gctcgtccgt gcgcggaaac agacgctcct cgatgttctg cagcaggatc gtgttcaact
     3361 gcgtgaccac cttcgcggcc cagtagtagc gctgcatcag acgctcgctg gcgcgatggg
     3421 tagcgctggg ctggataccc attgcctcgg ccaccgcgcc ctgcacgtcg aacaccaacc
     3481 gatcctcgcg ccggcccgcg accagatgca ggcgcgcccg gatctcgcgc agcgtgcgtt
     3541 cgttgcgcgt cagcaggcgg aactcctcgg cggtcagcag gccgcgcgcc gcgagctggc
     3601 gccacgacgc gccgaagccc gccgcgcgcg cgatccagag catcacctgc agatcgcgca
     3661 ggccgccggg actctccttg cagttgggct cgagcgcgta cggcgtctcc tggtacttcg
     3721 tatggcgctg ctgctgctcg agcagcttgg cgcggaaata agcctgtgag tcgagcgcgc
     3781 tgatgatcgc gcgcgaaaag tcgtcgaaga tcccggccgg cccggcgatc agtcgcgact
     3841 cgagcatcgc ggtgagcacg gtgacgtcgc gccgtgcctc ctcgcggcac tcttcgaggg
     3901 tgcgcacgct gtggccgagg tgcagcccgg tgtcccacag cgtgccgatc aaccgctcga
     3961 tgcgcgcgcg cccatcgaca tcgagcgcag cgcgcggcag cagcaggatg tcgacatcgg
     4021 agtgcgggaa gagctcgcca cggccgtagc cgccgacggc gatgaccgcc acatcggaag
     4081 tgatgccact cgcttccacg acccgccgca acattcggtc gaccgcccgc gccagtccct
     4141 tcaacagctg actgaccgat ccgccgtccc ggaagcgtgc gatcaggcca tcgcgctccg
     4201 cgcgcagctc gctgcgcagc gcgaaaccga cgtcgttcat tctgagggct tcaggccggc
     4261 gcgagcgcgg gcgtggccga cgccagcacg aaccgcggcg gcgcaggcgc gcccgccgac
     4321 agcgtcagga tctcgtagcc cgcttcggtg acgagcacgg tgtgctccca ctgcgccgat
     4381 agcgagtgat ccttggtgac gatggtccag ccgtcggcaa gctcgcggat ctcgcgccgg
     4441 cccgcattga tcatgggctc gatggtgaag atcatgcccg ccgccagccg ttccagcgtg
     4501 ccgggcttgc cgtagtgcag gacctggggc tcctcgtgga atttgcgacc gatgccgtga
     4561 ccgcagaact cgcgcaccac gctgtagcca tgcttttccg cgtgcgtctg aatcgcgtgc
     4621 ccgatgtcgc ccaggaaggc gccggcacgc acctggccga tgccgagcca catgcactcg
     4681 taggtgatct cgcacagccg gcgcgcggcg atcgacggct cgccgacata gaacatccgg
     4741 ctcgtgtcgc cgtgatagcc gtccttgatc accgtgatgt cgatgttgac gatgtcgccg
     4801 ttcttcagca cgcggtcgcc cggtatcccg tggcacacct ggtggttgac cgacgtgcag
     4861 atcgacttcg gatacggccg gtatcccggc ggcgcgtagt tcagcggcgc cggcgtggcg
     4921 ccctgtgtgt tgacgatcag gtcgtggcaa agccggtcga gttcgccggt cgtcacgccg
     4981 ggcttgacgt gcggcgtgat gaagtcgagc acttcgccgg ccagccggcc ggcgacacgc
     5041 atgccgtcga tatcggcggc gttcttgatg acgatgg
//
LOCUS       DBNHBY010000727         1854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_258259, whole genome shotgun sequence.
ACCESSION   DBNHBY010000727 DBNHBY010000000
VERSION     DBNHBY010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_258259"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16HB_17140"
     CDS             complement(<1..246)
                     /locus_tag="AC16HB_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608569.1"
                     /translation="MSEPVNAQTIADQRGQRVSNLKLLFVHSRVGRRLMSLFLLSALV
                     PVIVLGASLFARMSDFLRESNTAELAQAARTFGDVTLE"
     gene            complement(445..1479)
                     /locus_tag="AC16HB_17145"
     CDS             complement(445..1479)
                     /locus_tag="AC16HB_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608570.1"
                     /translation="MSEFTVAFHGVTKSSEAAARSMLRLLLPMLGNRWRIGDAASAEV
                     VILDAGSLEELNRTGTARETTLYIVFDDSGTPPANAFCVLHRPLNSARTIEVLHKAQA
                     EIEQRRGGMSPTTTLPAGFGADSSALERGIRTSLRTAVRWVLQDATSAVTVMSVRDTK
                     IFSALPGRGFTSRLTLTEIADLMRKNEPVKLLNLSEEEQQALAERKGDSFEPLLKLDW
                     IYWLTGTNGELRPELHVSKLYRLRQWPDFSRLPHYRADVRMASLLKVEALTVGQLAER
                     ADVRLETACNFVNGCAALGLLAGARPRAESAESNSAEAASKPTPARETREEPAGLLGT
                     LRSALGLARR"
     gene            complement(1496..>1854)
                     /locus_tag="AC16HB_17150"
     CDS             complement(1496..>1854)
                     /locus_tag="AC16HB_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5608571.1"
                     /translation="TDKDAATIAAAIINMAHTLRKEVVAEGVETAEQLAFLRAAGCEK
                     VQGYLLSPPLPADRLAALARCAESGGVAAVIEETRRRMSGATVLESAPITAAPPDLSN
                     LVTVPVMDPAQPLRVA"
BASE COUNT          276 a          637 c          624 g          317 t
ORIGIN      
        1 ctccagcgtg acgtcgccga aggtccgcgc tgcctgcgcg agctcggcgg tgttggactc
       61 gcgcaggaaa tcgctcatgc gagcgaacag ggatgcgccg agcacgatca ccggcaccag
      121 cgcggaaagc aggaacagcg acatcagccg gcgtccgacc cggctgtgca cgaacagcag
      181 cttcaggttc gacacgcgct gcccgcgctg atccgcgatg gtctgtgcgt tgaccggttc
      241 gctcattctt tgtcgtgctg acgccggctg ccgccacgcg gaggcgccag aagttgcgaa
      301 gggaagatca gcctagctcg ccgtgcttgg tgcggggatg caccctgtcc ggatcggtcg
      361 tccgtccttc ggttgcatct cgttcgcacg aatcggttgc ccacggtaac cgtcgggtcg
      421 atcggtggca gctctgaccg caccctagcg tcgcgccagg cccaacgcgc tgcgcagcgt
      481 tcccagcagg ccggcaggct cctcgcgcgt ctccctcgca ggcgtcggct tcgatgcagc
      541 ctcggcggaa ttactctccg cactctccgc gcgcggccgc gcacccgcga gcaggcccag
      601 cgcggcgcag ccgttgacga agttgcacgc agtctcgagc cgcacgtcag cgcgctcggc
      661 gagctgtccg accgtcagtg cctcgacctt cagcaacgag gccatgcgca cgtcggctcg
      721 atagtgcggc agccggctga agtcgggcca ctggcgcagc cggtagagct tgctgacgtg
      781 caattcgggc cgcagttcgc cgttggtgcc ggtgagccag tagatccagt cgagcttcag
      841 cagcggctcg aaactgtcac ccttgcgctc ggccagcgcc tgctgctctt cttcactcag
      901 gttcagcagt ttgacgggct cgttcttgcg catcagatcg gcaatctcgg tcagcgtcag
      961 ccgagacgtg aagccccgcc ccggcagcgc ggagaagatc ttggtgtccc gcacgctcat
     1021 caccgtgacc gcgctcgttg catcctgcag cacccagcgc accgcggtgc gaagcgacgt
     1081 gcggatgccg cgttcgagcg ccgacgagtc ggcgccgaac ccggcgggca gcgtcgtcgt
     1141 cggactcatg ccaccgcgcc gctgctcgat ttcggcctgc gccttgtgca gcacctcgat
     1201 cgtgcgcgcg ctgttcagcg gccggtgcaa cacgcagaag gcgttggccg gcggcgtgcc
     1261 cgagtcgtcg aacacgatgt acagcgtggt ttcacgcgcg gtgccggtgc ggttcagttc
     1321 ctcgagcgaa ccggcatcga ggatcacgac ctcggcgctc gcggcgtcgc cgatgcgcca
     1381 gcggttgccg agcatcggca gcagcaggcg cagcatcgag cgtgccgccg cttccgatga
     1441 cttggtgacg ccgtgaaatg caacggtgaa ttcgctcatg gtcgttgtct ccgtctcaag
     1501 cgacacgtag cggttgggcc gggtccatga ccggcacggt tacgaggttg gacaggtcgg
     1561 gcggtgccgc agtgatgggc gcggattcga gcacggtggc gccgctcatg cgccggcgcg
     1621 tttcctcgat cacggccgcc acgccgccgg attccgcgca acgcgccagc gccgccagac
     1681 gatcggccgg cagcggcggg ctgagcaggt agccttgtac cttctcgcag ccggcggcac
     1741 gcagaaaggc gagctgctcg gccgtctcga cgccctcggc cacgacctcc ttgcgcagcg
     1801 tgtgcgccat gttgatgatc gccgccgcga tcgtcgccgc gtccttgtcg gtgg
//
LOCUS       DBNHBY010000728         5475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_459821, whole genome shotgun sequence.
ACCESSION   DBNHBY010000728 DBNHBY010000000
VERSION     DBNHBY010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_459821"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="AC16HB_17155"
     CDS             complement(<1..999)
                     /locus_tag="AC16HB_17155"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5608572.1"
                     /translation="MHYRLTLSVAALAAAAALSLPASVVAVQPAAAGDAASKNRVSNS
                     AYIVQLAEDPVVAYRGNIKGYAATAPGQLQKIDPYDPRVVAYMSYLASRQDALLASVG
                     GGRKIYGYGYAFSGFAAELSPAQAAKLAQTPGVLAVTKDVLVDQDTSSTPAFLGLDAP
                     GGLWDQLGGDGNKKDAGAGEGIIIGIVDSGVWPQSRSFSDRDANGKLAYQQIPGWNGK
                     CVPGEGFNGSDCNQKLIGAQYFNAAWGGNAGIDSERPWEFNSPRDYNGHGTHTASTAG
                     GNRGVQTTGPASVFGAISGMAPRARIAAYKVLWSTQDGSLASGFTTDIVAAIDQAVA"
     regulatory      complement(1156..1242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1354..2739)
                     /locus_tag="AC16HB_17160"
     CDS             complement(1354..2739)
                     /locus_tag="AC16HB_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR5608573.1"
                     /translation="MTRNRKSRPIDGSRRQFLQTASALSVLGSATPLALNLAGIGAAA
                     AQSAEDYRALVCVFLFGGNDQTNTVVPFDPAEFNTYTAARPSIALTSSQLTSLGAVAA
                     QGGRSFALPNELAPLATLYGSGKLAVVANVGPLIVPTTKSQYQSASVPLPPKLFSHND
                     QQSVWQSSGAEGATSGWGGRIADLIASGNANQTFSAVSVAGAAVYLSGRTVLQYQVGT
                     NGPVAISGLTGSLFGSGTAPQVLQQLITAARPGMFEDEYDRVTARSIAANTTLTGALA
                     SAPALTTAFPTGNSLADQLKMVARTISGRQALGMKRQVFFVSQGGFDTHDFQLRDQPG
                     LHATLAAALEAFYNATVELGVADLVTTFTASDFGRTLTSNGDGSDHGWGSHHFVLGGA
                     VRGSSIYGAFPAIALNTAEDVGSGRLLPSTAVDQYAATLARWFGVTDADLATVVPNVA
                     NFATRNLGFLG"
     gene            complement(2751..4514)
                     /locus_tag="AC16HB_17165"
     CDS             complement(2751..4514)
                     /locus_tag="AC16HB_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR5608574.1"
                     /translation="MEETTAQEAVAPRNDEVRPPDSAAPLKLVAPLFAAAAAAACGGG
                     SGDASNPPPAPTPTPSPPPPGPTISQADAVRFLTQASFGATDATIQSVIALGYGGWLD
                     DQFGRARALHRTYLEQAIAAGDPAAPESTYRNHVMDTFWTQAITGEDQLRQRMAFALS
                     QIFVVSQANADVNNRPRGLADYLDMLGTHAFGNFRALIEAVSLHPIMGLYLSALRNQK
                     EDPATGRVPDENYAREVMQLFTIGLYQLNTDGTLKRDGSGNPIPTYGNADVQGLAKVF
                     SGWSWAGPDTTNTRFFGGNADPNRDVTPMQAYPQFHSASAKTFLGVTIPANTPAAPSL
                     KTALDALFNHPNTAPFFARQLIQRFVTSNPSPAYVARVAAAFADNGAGQRGDMKAVIR
                     ALLLDAEARSAGIAAQPTWGKLREPVIRLANWARAFGATSVSGNYMIRNVADPSTALG
                     QNPLRSPSVFNFYRPGYVPPSTAIATAGLVAPEFQITGETSVAGYLNFMRNTVNAGAG
                     TGTDVRSAYTQEIALAGNPDQLVDRVKLVLTAGQMSDATRTAIRDAVASIQTSAPNAL
                     VNRAKLAIYLTLASPEFIVQK"
     gene            4652..>5475
                     /locus_tag="AC16HB_17170"
     CDS             4652..>5475
                     /locus_tag="AC16HB_17170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5608575.1"
                     /translation="MSLLDRLNPQQLAAVTLPHQPALILAGAGSGKTRVLTTRIAWLI
                     QTGQVSPQGILAVTFTNKAAREMLTRIAAMLPINVRGMWVGTFHGLCNRLLRAHHRDA
                     SLPQAFQILDATDQLSAIKRLLKGNHIDDEKYPPRNLQLFINSAKEAGLRANAVDVND
                     DYNRKFVELYSLYDAQCQREGVVDFAELLLRCYELLVKNEPLRLHYQERFRHILVDEF
                     QDTNVLQYKWLKLLAGWSGSDGSLPVAHNAIFAVGDDDQSIYRFRGANVGNMADFER"
BASE COUNT          870 a         1822 c         1845 g          938 t
ORIGIN      
        1 ggcgaccgcc tggtcgatcg ccgcgacgat gtcggtggtg aagccgctcg caagcgaccc
       61 atcctgcgtc gaccacagca ccttgtacgc ggcgattcgt gcgcgcggcg ccatgccgct
      121 gatcgcgccg aacaccgacg cagggccggt cgtctgcacg ccgcggttgc cgccggcggt
      181 cgaagccgtg tgcgtgccgt gaccgttgta gtcacgcggc gagttgaact cccacggccg
      241 ctcggaatcg atgccggcgt ttccacccca ggcggcgttg aaatattgcg caccgatcag
      301 cttctggttg cagtccgacc cgttgaaccc ctcgcccggc acgcacttac cgttccagcc
      361 cggaatctgc tggtacgcga gcttgccgtt cgcatcgcgg tcgctgaagc tcctgctctg
      421 cggccacacg cccgagtcga cgatgccgat gatgatgcct tcgcccgcgc cggcgtcctt
      481 cttgttgccg tcgccgccga gctgatccca cagcccgcca ggagcgtcga gtccgagaaa
      541 cgccggcgtc gatgacgtgt cctggtcgac cagcacgtcc ttggtgaccg cgagcacgcc
      601 cggggtctgc gccagcttcg ccgcttgcgc gggcgaaagc tctgccgcga agccgctgaa
      661 cgcgtacccg taaccataga tctttctccc gccgccaacg ctcgccagaa gcgcgtcctg
      721 gcgcgacgcc aggtagctca tgtaggccac gacgcgcgga tcgtacgggt cgatcttctg
      781 aagctgacca ggtgcggtag ccgcgtagcc tttgatgttg ccgcgatagg cgacgacggg
      841 gtcctcggcc agctgcacga tgtaggcgct gttgctgaca cggttcttgc tggcggcgtc
      901 gcccgcggcc gccggttgca cggcgacgac gctggccggc aacgacaacg cggcagccgc
      961 ggcgagcgcg gccaccgaca gggtcagtcg gtaatgcata ggcacctctc ctcctgcact
     1021 gttgagttga gttcgagttc ccacgcgagt cggagaatcg cgcgcgcgcc atccgtgtct
     1081 cgacgcgctg cttacccaac atcaggagtc agtgccggcc gcgcacgagt gcagcgcgac
     1141 gcgtcggttg tctcccttgg caacccagcc ctcttgttgt cgtgcttagc aagagctgtg
     1201 gctttgcggc cccgcttcgc agcgggtgtg cccttcgacc gttgcagtgc gcggaacgta
     1261 ctcttcgccc gtgacaaagg caaccgttcc gcgacgccaa tgtgactatt cgtcgacgcg
     1321 aagaatgtga gtaagtcctt gcggaactcc gcgtcagccg aggaacccga gattgcgcgt
     1381 agcgaagttg gcgacgttgg gcaccacggt ggccagatcc gcgtcggtga cgccgaacca
     1441 gcgcgccagg gtcgccgcat actgatcgac cgcagtcgac ggcagcaacc gtccggagcc
     1501 gacgtcctcg gccgtgttca gggcaatggc cggaaacgcg ccgtagatgc tgctgccgcg
     1561 gactgcgccg ccgagcacga agtgatgcga accccagccg tgatcggagc cgtcgccgtt
     1621 gctggtcagc gtgcggccga aatcggatgc ggtgaatgtc gtgacgagat ctgccacgcc
     1681 gagttcgacc gtagcgttgt agaacgcctc gagcgcggcg gcgagcgtcg cgtgcaggcc
     1741 gggttgatcg cgcaactgga aatcatgcgt gtcgaacccg ccctggctga cgaagaacac
     1801 ctggcgcttc atgcccagcg cctgccgccc gctgatcgtg cgggcgacca tcttcagttg
     1861 atcggcgagc gagtttccgg tcggaaaagc ggtcgtcagc gccggcgcag acgccagcgc
     1921 gccggtcagc gtggtgttgg ccgcgatcga gcgcgctgtc acgcggtcgt attcgtcctc
     1981 gaacatgccg ggccgcgctg cggtgatcag ctgctgcaac acctggggcg cggtgccgga
     2041 gccgaacaag ctgccggtca atccgctgat cgccaccggg ccattggttc cgacctggta
     2101 ctgcagcacg gtgcggccgg acagatacac ggcggcacca gcgaccgaga ccgcgctgaa
     2161 ggtctggttc gcgttgccgg acgcgatcag gtcggcgatg cgcccgcccc agccgctggt
     2221 tgcgccctcg gcgccggacg attgccaaac cgactgctgg tcgttgtgcg aaaagagctt
     2281 gggcggcaac ggcaccgatg cgctctggta ctgcgacttc gtcgtcggca cgatcagcgg
     2341 cccgacgttg gcgacgacgg caagtttgcc gcttccgtac agcgtggcca acggtgccag
     2401 ttcgttcggc agcgcgaaac tgcgaccgcc ctgggcagcg acggcgccga gcgaggtcaa
     2461 ttgcgacgac gtgagcgcga tgctcggacg cgccgccgtg taggtgttga actctgccgg
     2521 gtcgaacgga acgaccgtgt tggtctggtc gttgccgccg aacaggaaca cgcacaccag
     2581 cgcgcggtag tcctccgcgc tctgcgccgc cgccgcaccg atgccggcga gattcagcgc
     2641 cagcggcgtg gcactgccga gcaccgacag cgccgaggcg gtctgcagga actggcggcg
     2701 gctgccgtcg atcggacgcg actttcggtt tctggtcatg gacaagctcc ctacttctga
     2761 acgatgaact cgggcgacgc cagcgtcaga tagatcgcga gcttggcgcg attcacgagc
     2821 gcgttgggcg ctgaggtctg gatcgaagcc actgcatcgc ggatcgcggt gcgcgtcgca
     2881 tcgctcatct ggccggcggt cagcaccagc ttcacgcggt cgaccagctg gtccgggttg
     2941 ccggcgagcg cgatttcctg tgtgtacgcg gaacgcacgt cggtgccggt tcctgcgccg
     3001 gcgttcaccg tgttgcgcat gaagttcaga tatccggcaa ccgacgtctc gccggtgatc
     3061 tggaattccg gcgcgaccag ccccgcagtt gcgatcgccg tggacggcgg cacgtagccg
     3121 ggccgataga agttgaacac cgacggcgag cgcaacgggt tctgtccgag cgcggtcgac
     3181 gggtcggcaa cgttgcggat catgtagttg ccgctcaccg aggttgcgcc gaacgcgcgc
     3241 gcccagttgg cgagccggat caccggctcg cgcagcttgc cccatgtcgg ctgtgccgcg
     3301 atgccggcgc tgcgcgcttc ggcgtcgagc agcagcgcgc gaatcaccgc tttcatgtcg
     3361 ccgcgctggc cggcgccgtt gtccgcgaac gcggcggcca cgcgcgcgac gtacgcgggc
     3421 gacgggttgc tggtgacgaa gcgctggatc agttgccgag cgaagaacgg cgcggtgttc
     3481 ggatgattga acagcgcatc gagtgcggtc ttcaggctcg gggccgccgg cgtgttggca
     3541 ggaatcgtga cgccaaggaa ggtcttggcc gacgccgaat ggaactgcgg gtacgcctgc
     3601 atcggcgtca cgtcccggtt cgggtccgcg ttgccgccaa agaagcgcgt gttcgtcgtg
     3661 tccggccccg cccagctcca gcccgagaag accttggcga gtccctgcac atcggcgttg
     3721 ccgtacgtcg ggatcggatt gccgctgccg tcgcgcttca gcgtgccgtc ggtgttgagt
     3781 tggtacaggc cgatcgtgaa aagctgcatc acttcgcgcg cgtagttctc gtccgggacg
     3841 cggccggtcg cggggtcttc cttctggttg cgcagcgcgg acaggtacag ccccatgatc
     3901 gggtgcaggc tgaccgcctc gatcagcgcg cggaagttgc cgaacgcatg cgtgcccagc
     3961 atgtcgaggt agtcggccag tccgcgcgga cggttgttca cgtcggcatt ggcctgcgac
     4021 accacgaaga tctgcgacag cgcaaatgcc atccgctgcc gcaactggtc ctcgcccgtg
     4081 atcgcctgcg tccagaacgt gtccatgacg tggttgcgat acgtgctctc cggggcggcc
     4141 ggatcgccgg ccgcgatcgc ctgctcgaga taggtccggt gcagcgcgcg cgcccgtccg
     4201 aactgatcgt cgagccagcc gccgtagccg agcgcgatca ccgactgaat cgtcgcgtcg
     4261 gtcgcgccga agctcgcctg cgtgagaaag cgcacggcgt ccgcctgcga gatcgtcggt
     4321 ccgggaggcg gtggcgaagg cgtcggtgtc ggcgcgggag gcgggttcga cgcatcgccg
     4381 ctgccgccgc cgcacgcggc cgcagctgca gcggcgaaca gcggcgcgac gagtttcagc
     4441 ggcgccgcag aatcgggcgg ccggacttcg tcgttccgtg gcgcgacggc ttcctgagcg
     4501 gtcgtttctt ccatcgtctt ctcctgccgg actgccgcgc aatgtgccgg cactgcgccg
     4561 ccccgccaat cgttgataca cggtgttacc ccacttacgc actcgcgtcg aatcgggtcg
     4621 gtcgttcgat cgaccgtaga atgcgatcct catgtcgttg ctcgatcggc tcaatccgca
     4681 gcagctcgcc gcggtcacgc tcccgcatca acccgcgctg atcctggccg gcgccggctc
     4741 gggcaagacg cgcgtgctga ccacgcgcat cgcctggctg attcagaccg ggcaggtctc
     4801 gccgcagggc atcctcgcgg tgacgttcac caacaaggcg gcccgggaga tgctgacgcg
     4861 catcgcggcg atgctgccga tcaacgtgcg cggcatgtgg gtcggcacgt ttcacggcct
     4921 gtgcaaccgg ctgctgcgcg cgcatcaccg cgacgcgagc ctgccgcagg cgttccagat
     4981 cctcgatgcc accgaccagc tgtcggcgat caagcggctg ctgaagggca atcacatcga
     5041 cgacgaaaag tacccgccgc gcaacctgca gctcttcatc aacagtgcaa aggaagccgg
     5101 gctgcgtgcg aacgcggtcg acgtcaacga cgactacaac cgcaagttcg tcgagctgta
     5161 ttcgctgtac gacgcgcagt gccagcgtga gggcgtcgtc gacttcgccg agctgctgct
     5221 gcgctgctac gagctgctcg tgaagaacga gccgctacgg ttgcactacc aggagcgctt
     5281 ccgccacatc ctggtcgacg agttccagga caccaacgtg ctgcagtaca agtggctgaa
     5341 gctgctggcc ggctggagtg gaagcgacgg gtccctgccc gtcgcacaca acgcgatctt
     5401 cgcggtcggc gacgacgacc agtcgatcta tcgctttcgc ggcgccaacg tcggcaacat
     5461 ggccgacttc gaacg
//
LOCUS       DBNHBY010000729         2854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1139333, whole genome shotgun sequence.
ACCESSION   DBNHBY010000729 DBNHBY010000000
VERSION     DBNHBY010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1139333"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="AC16HB_17175"
     CDS             <1..548
                     /locus_tag="AC16HB_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608576.1"
                     /translation="TGKRDFDKAKALVKEAGYKGEKIILMTATDQPIVHAQALVTQEM
                     LRKIGLNVELAAMDWGTLITRRSSKEPVDKGGWSIFHTWFVGPDITSPALNSPLRGAG
                     DKSWFGWPTDAKLEELRDAWFAAPTPAEQKKVAEEMQRRAFETVPYIPTAQFIIPTAY
                     RTNLSGVIVSPVTFLWNVEKK"
     gene            633..1574
                     /locus_tag="AC16HB_17180"
     CDS             633..1574
                     /locus_tag="AC16HB_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608577.1"
                     /translation="MLRYIVKRLLATIPVMAVVALFVFSLLYISPGDPAAVIAGDIAT
                     AEDIARIRAQLGLDQPYLVRFGHWLWSLAHGDLGISIFTNLPVSTLIAQRIEPTVALT
                     ICTLVVSVGLAVPLGVLAAARAGGWIDRVVMALSVVGFSVPVFVIAYGLILLFSIQLD
                     WLPVQGYRSIGEGFGPFIRHMILPSVALGLVYMALIARITRASMLDVLAQDYVRTANA
                     KGLAPGTVLTRHALKNAAVPIVTIIGIGVALLIGGVVVTETVFAIPGIGRLTVDAILR
                     RDYPIIQGVTLLFSAVYVIVNLLVDLSYSLFDPRIRY"
     gene            1576..2454
                     /locus_tag="AC16HB_17185"
     CDS             1576..2454
                     /locus_tag="AC16HB_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608578.1"
                     /translation="MAAVAIEVPKSASPTWLRFKDAFRRHPTAIAGAIVLLLMVVIAL
                     AAPWLGTTDPLAVSPIRRLRPPGEQYWFGTDMLGRDVYSRVIYGARVSLTVGLAVALL
                     STVIGLAIGLVTGFVRLLDAIMMRVMDGLMSIPSVLLAIALISLTTASLKNVIVAIAI
                     TEIPRVVRLVRSLVLTLREQPYVEAAIASGTRLPAILLRHILPNTLAPLLVQATYICA
                     SAMITEAILSFIGAGTPPNIPSWGNIMAEARSLIQVAGTILLFPGIFLSLTVLSVNLL
                     CDGMRDALDPRLSRRM"
     gene            complement(2551..>2854)
                     /locus_tag="AC16HB_17190"
     CDS             complement(2551..>2854)
                     /locus_tag="AC16HB_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5608579.1"
                     /translation="KDSTLALVGESGSGKSVTSLAILGLLPPENSIVDSKSAIVYGGK
                     NLVGLPMRQLRDLRGADISMIFQEPMTSLNPVFTVGYQLEEVLKLHMGMTGRRARR"
BASE COUNT          417 a          952 c          956 g          529 t
ORIGIN      
        1 tgaccggcaa gcgcgatttc gacaaggcca aggcgctggt gaaggaagcc ggctacaagg
       61 gtgagaagat catcctgatg acggcgaccg accagccgat cgtgcatgcg caggcgctgg
      121 tcacgcagga gatgctgcgc aagatcggcc tgaacgtcga actggcggcg atggactggg
      181 gcacgctgat cacgcgccgc tcgtcgaagg agccggtcga caagggcggc tggagcatct
      241 tccacacctg gttcgtcggc ccggacatca cgtcgccggc gctcaattcg ccgctgcgcg
      301 gggcgggcga caaatcctgg ttcggctggc ccaccgatgc caagctcgaa gagctgcgcg
      361 acgcctggtt cgccgcgccg acgccggccg agcagaagaa ggtcgccgag gagatgcaga
      421 ggcgcgcgtt cgagaccgtg ccgtacatcc cgaccgcgca gttcatcatc ccgaccgcgt
      481 atcgcaccaa cctgtccggc gtgatcgtgt cgccggtgac gttcctctgg aacgtggaga
      541 agaaatagtg cggactgccc cacgctgaaa cgtggggcaa ctcggcgcgg cagtgcctcg
      601 gtgctgccgc gtttgcgttt tttggcgacc cgatgcttcg ctacatcgtc aaacgcctgc
      661 tcgcgacgat ccccgtgatg gcggtcgtcg cgctgttcgt cttttcgctg ctgtacatca
      721 gccccggcga cccggcggcc gtgatcgcgg gcgacatcgc cacggcggag gacatcgccc
      781 gcatccgcgc gcaactcggg ctcgaccagc cctacctggt gcgcttcggc cattggctgt
      841 ggtcgctggc gcacggtgac ctcggcatct cgatcttcac caacctgccg gtatcgacgc
      901 tgatcgcgca gcggatcgag cccaccgtcg cgctgacgat ctgcacgctg gtcgtgtcgg
      961 tcgggctcgc ggtgccgctt ggcgtgctgg ctgcagcgcg tgcgggcggc tggatcgacc
     1021 gcgtggtgat ggcgttgtcg gtggtcggtt tctcggtgcc ggtgttcgtg atcgcgtacg
     1081 ggctgatcct gctgttctcg attcagctcg actggctgcc cgtacagggc taccgcagca
     1141 tcggcgaggg cttcggcccg ttcatccgcc acatgatcct gccgagcgtg gcgctcgggc
     1201 tcgtctacat ggcgctgatc gcgcgcatca cgcgcgccag catgctcgac gtgctggcgc
     1261 aggactacgt gcgcaccgcg aacgccaagg gactcgcgcc cggcaccgtg ctgacgcgcc
     1321 atgcgctgaa gaacgccgcg gtgccgatcg tcacgatcat cggcatcggt gtcgccttgc
     1381 tgatcggcgg cgtggttgtg accgagaccg tgttcgcgat ccccggcatc ggccggctga
     1441 ccgtggacgc gatcctgcgc cgcgactacc cgatcatcca gggcgtcacg ctgctgttct
     1501 ccgccgtgta cgtgatcgtc aacctgctgg tcgatctgag ctactcgctg ttcgatccgc
     1561 ggatccgcta ctgacatggc tgcggtcgcg atcgaggtac cgaagtccgc gtcgcccact
     1621 tggctgcgct tcaaggatgc gttccggcgg catccgactg ccatcgccgg tgcgatcgtg
     1681 ctcctgctga tggtcgtgat cgcgctcgcc gcgccgtggc tcggcaccac ggatccgctg
     1741 gcggtctctc cgatccgccg gctgcgcccg ccgggcgagc agtactggtt cggcaccgac
     1801 atgctggggc gcgatgtcta cagccgcgtg atctatggcg cgcgcgtgtc gctgacggtg
     1861 gggctggcgg tggcgctgct gtcgacggtc atcggcctgg cgatcgggct cgtgaccgga
     1921 ttcgtgcggc tgctggacgc gatcatgatg cgcgtgatgg acgggctgat gtcgatcccg
     1981 tcggtgctgc tggcgatcgc gctgatctcg ctcaccaccg cgagcctgaa aaacgtgatc
     2041 gtcgcgatcg cgatcaccga gatcccgcgc gtggtgcggc tggtgcgctc gctggtgctg
     2101 acgctgcgcg agcagccgta cgtcgaggcg gcgatcgcct cgggaaccag gctgccggcg
     2161 atcctgctgc gccacatcct gccgaatacg ctggcgccct tgctggtgca ggcaacctac
     2221 atctgcgcct cggcgatgat caccgaggcg atcctgtcct tcatcggcgc cggcacgccg
     2281 cccaacatcc cgagctgggg caacatcatg gccgaggcga gaagcctgat ccaggttgcc
     2341 ggcacgatcc tgctcttccc cggcatcttc ctttcgctga ccgtgctgtc ggtgaatctg
     2401 ctctgcgacg gcatgcgcga cgccctcgat ccgcgcctct cgcggcggat gtgatggacg
     2461 ccgcggccga accgctgctc gaggtcgaag gcctgcgcac gtatttctac acgcgcgacg
     2521 gcgtcgtgcg cgccgtggat ggcgtgtcgt tcaccgtcgc gcgcggcggc ccgtcatgcc
     2581 catgtgcagc ttcagcactt cctcgagctg atagccgacg gtgaacaccg ggttcagcga
     2641 cgtcatcggc tcctggaaga tcatcgagat gtcggcgccg cgcagatcgc gcagctggcg
     2701 catcggcaat ccgaccagat tcttgccgcc gtagacgatc gcgctcttcg aatcgacaat
     2761 cgagttctcg ggcggcagca ggccgaggat cgcgagcgat gtcaccgact tgccggatcc
     2821 cgattccccg acgagcgcaa gcgtgctgtc cttc
//
LOCUS       DBNHBY010000730        10714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1340928, whole genome shotgun sequence.
ACCESSION   DBNHBY010000730 DBNHBY010000000
VERSION     DBNHBY010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1340928"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="AC16HB_17195"
     CDS             complement(<1..866)
                     /locus_tag="AC16HB_17195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608580.1"
                     /translation="MSSRPPPATRRRHTGAWAPLAEPVFRALWIASLGSNIGTWLQNV
                     AAGWEMTNLTPSPLLVALVQVAITAPSFLLAIGAGALADIADRRAILIGTQAAGAVIA
                     GLLAFATALGALTAPTLLLLTFLLGAQWALTIPAWGATVADIVPRAQLASAVALGSIS
                     FNISRSIGPALGGLILSISHPSVAFALNAMSYVGVLVVLLRWQRARPVPRLPPEGFAA
                     AIRAGLRYTLEEPALRALLVRCAVFSAPAAAVFSLLPVIAKGSTFSSANGYAFLLTAI
                     GIGAVATALALPR"
     gene            993..1481
                     /locus_tag="AC16HB_17200"
     CDS             993..1481
                     /locus_tag="AC16HB_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5608581.1"
                     /translation="MPRTTPQPPRRARRQYRGDRYRIEESIGYLMKLALSALRRRLDR
                     RMAEHDLTGVQMLPLFLIGRGVCGTAAELARRNDIDAGATTRLLDRLEAKGLLRRSRS
                     AQDRRVVQLELTAAGRAIADQIPYALADTLNEALRDFSATEVETLRSMLRRIVANTEG
                     AA"
     gene            1478..2974
                     /locus_tag="AC16HB_17205"
     CDS             1478..2974
                     /locus_tag="AC16HB_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5608582.1"
                     /translation="MKRRATLLATALLLAGCASFDGIAPGVAQRTAAQLGATPSDAAW
                     PRDDWWTWFGDRQLDTLIERALAGNPQLDVARARLARAQAAVAASGAAQKPHLDAQAQ
                     STWQRFNENFLYPPPLAGSTDSSNTLQLTGSWELDFFGRHRAALRAALSQSRAVAAEA
                     QAARLLITASVARTYMQLARLVETRDVLAATLAQREQIVRLVQARVKSGLDTQVELRQ
                     AEAAVPEIRQQREALDEQMQIARHALAALLGAGPEATQALAPRLAQAQPPPLPATIPA
                     ELMGRRADLSAARWRVEAAAADVDAAKAQFYPNVNLLAFAGLQSLGFAHWLDAGSRTF
                     GAGPAVSLPVFDAGRLRAALRGRTADFDAAVADYNAALTDAVRDVADQIASLQSLERQ
                     AAEQRAAQTATEGAYELALLRYRAGLGSYLAVLVAETSVLAQRRIGTDLKARRVDASI
                     ELMRALGGGYRADADRETRGGDPADPATSDRGDADAALAARTNLPEPR"
     gene            2971..4224
                     /locus_tag="AC16HB_17210"
     CDS             2971..4224
                     /locus_tag="AC16HB_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrA/EmrK family multidrug efflux transporter
                     periplasmic adaptor subunit"
                     /protein_id="HIR5608583.1"
                     /translation="MSATDPDKSNTRPRRVALAALAGTFGLAALGYGAYWYLFERNLE
                     TTDNAYVAGNLVQITPQIGGTVLAIQADETDFVRAGQPLVNLDRADAQVALEQAQAQL
                     AQTVREVRTLYANNASLAAVVALRDAEVAKARSGIAKAQDDLARRQSLAATGAVSDEE
                     IKHAATALDDAKSVLAAAQAAGSAAREQLAASQALTAGTTVHDHPSVQRAAARVREAH
                     LALSRVELPAPVAGYVAKRSVQVGSRVQVGAPLMTIVPLDQVWVEANFKEVQLRSLRI
                     GQPAHVTADLYGTKVEYHGTVAGLGVGTGSAFALLPAQNATGNWIKVVQRVPVRIALD
                     TRELAAHPLRVGLSMEVTVDVADRSGKLLADAPRAQPVATTESFAPPTREADAMIERI
                     IAANLAGAPAPRAPAEVAEPASSRR"
     gene            4249..5805
                     /locus_tag="AC16HB_17215"
     CDS             4249..5805
                     /locus_tag="AC16HB_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR5608584.1"
                     /translation="MSAAARSHAHPPLAGGALVIGTLALSLATFMNVLDTSIANVSIP
                     AISGDLGVAPNQGTWVITSFGVANAISLPLTGWLTQRFGQVRLFCISVLLFVLASFLC
                     GLAPNLETLIAFRVMQGFVAGPMIPLSQTLLLASYPPRRSGTALAFWGMTTLIAPVVG
                     PLAGGWITDNIGWPWIFFINIPVGLAATIATWSIFRRRETPTRTVPIDGVGLGLLVLW
                     VGCLQVMLDKGRELDWFASGQIVALAVTAVVGFAFFLAWELTDAHPVVEVRLFARRNV
                     TFATIAIAVAYALFFGNLVLLPLWLQQWMGYTATQAGWALAPVGALAIVLTPVVGRYV
                     NRVDPRPIVTASFITFALVMYLRAQFTTDTDLATIMVPTVIQGAAVACFFIPLTSISL
                     SGLTPDRIASASGLVNFARITAGSFGTSIATTLWDHRASLHHSQLVEHLTRGNLPTER
                     FLESATRAGLTQEQALALLNRGVDTQSFTLAADEIFYVSALLFLALVGLVWLTRPQRG
                     GLANIDGGAH"
     gene            5896..6270
                     /locus_tag="AC16HB_17220"
     CDS             5896..6270
                     /locus_tag="AC16HB_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR5608585.1"
                     /translation="MMARTFLVLAALSGFIAVAAGAFGAHALKTRLAADLLAVFDTAV
                     RYQMFHVVGLIAAAWLAARSPGPAVLASGWCFVAGTVLFSGSLYALALTGTRWLGAIT
                     PFGGVLFLAGWLLLAWTAWRLD"
     gene            6289..7134
                     /locus_tag="AC16HB_17225"
     CDS             6289..7134
                     /locus_tag="AC16HB_17225"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5608586.1"
                     /translation="MAAAGEIENMAVFCDFENIALGVQDAKYEKFDVKAVLDKLLVKG
                     SIVVKKAYCDWARYKEFKAPMHEQAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKS
                     HVDTFVIVSGDSDFSPLVSKLRENNKTVIGVGVKASTSDLLIKNCDEFIFYDDLVRAG
                     EARRRQAAKRPKRVEPKKAEHRMAGEPAANDDDAARRRQAAIDLVLETLEELIEARGS
                     DGRIYASMVKQALKRRRPEFNESYHGYKSFGALIDDAATRGVLTVAKDEKTGQQLVRV
                     AAPDQ"
     gene            7383..8276
                     /locus_tag="AC16HB_17230"
     CDS             7383..8276
                     /locus_tag="AC16HB_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5608587.1"
                     /translation="MPVDGRRRITAYLLLTASMTLVGSYVALSKPLVAAIPVFALAFL
                     RFGIAAVSMLPWTLPRPHETPLTRREHGLFFLMSFLGNFLFSICMLSGVALTTATAAG
                     VIMATIPAVVAAGSWLFLRERLTPRVLAAIALAIGGIALLQFARASDASRQTTLLGNA
                     LIFGAVVCEASYVIIGKRLAATRTPLRVSALINLWGLALMTPFGLWQLARFDLGAVSV
                     PIWALLVFYSLAASLFAVWLWMTGLKHVPAHHAGVFTVALPIAATAVGVAFLGEQFTL
                     LHGGALLLASTGVVLIASARH"
     gene            complement(8284..9801)
                     /locus_tag="AC16HB_17235"
     CDS             complement(8284..9801)
                     /locus_tag="AC16HB_17235"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5608588.1"
                     /translation="MLRKLIWLLFFIASPAVAGDAIAWHDGDVDAAFAQAKREGKPLF
                     LYWGAVWCPPCNQVKATIFPRRDFIERSRFFVPVYLDGDAPNAQKIASRFKVGAYPTM
                     ILFRPDGTEITRLPGEVDGARYMQVLALGMNAGRPVRALLASAQTRPAALAANDWRLL
                     AFYSWETDHQALVPKRELAPVLWKLAQAGAAKAPDAAARLTLKAVSLAADAKDKDASG
                     IDKRVALARIEKTLGDDDLARANFVEFAYGAQDIVGYLTDANTPARAKLQRMWDRRLA
                     LFCDDAGLARLDRIAAADARVVLARLDVPKGALPMALQTEVRGVVLRANRDATDRFER
                     EAVVTASADLLADADLLAESDALLQAALARSAAPYYLMLGLASNARKRGDPATALNWY
                     EQAYATSQGPATRLQWGATYVRALIDLAPQDGARIERALAGVIADIEPKPETFYGRNR
                     AALDRISTKLGEWNKDGSHAAVLARLRAQLAAVCGKLPAGAPERATCEAVLATPV"
     gene            complement(9815..>10714)
                     /locus_tag="AC16HB_17240"
     CDS             complement(9815..>10714)
                     /locus_tag="AC16HB_17240"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HIR5608589.1"
                     /translation="ARARDGRELALKIQYPGVARSIASDVDNVAALLKLFNLLPFDVD
                     VDGIVAEAKRQLLQEADYRAEAGFLEHYARLVADEPLLIVPRVHRDFTTTRVMAMDYV
                     EGAPLESLAADVPPAQRDAVGTLLERLLFRELFEFRVMQTDPNFANYLVQPGGRVVLL
                     DFGATRAFDARFVANYARITRAVIEEDADAIAHYAVAIGYVTADDPVERVQAAVDVIR
                     LVCEPLRHRGPYDFAASDLPARGRALGIDLGLRRGLLRAPPPETMFLHRKLVGSFLLL
                     ARIGARVDARALALPLIERALAD"
BASE COUNT         1621 a         3825 c         3706 g         1562 t
ORIGIN      
        1 cgcggcagcg cgagcgcggt agccaccgca ccgatgccga tcgcggtcag caggaaggcg
       61 tacccgttgg cgctgctgaa ggtcgaaccc ttggcgatca ccggcaacag cgagaacacg
      121 gccgcggccg gcgcggagaa caccgcgcag cgaacgagca gcgcgcgcaa tgcgggttcc
      181 tccagcgtgt agcgcagtcc ggcgcggatc gcagcggcga atccctccgg cggcagccgc
      241 ggcactggac gcgcgcgctg ccagcgcaac agcacgacga gcacgcccac gtaggacatc
      301 gcgttcagcg cgaaggcgac cgacgggtgc gagatcgaca ggatcagccc gccgagcgcg
      361 gggccgatcg agcgcgagat gttgaacgag attgaaccga gcgcgacggc cgaggcgagt
      421 tgcgcgcgcg gcacgatgtc ggccaccgtc gcgccccacg ccgggatcgt cagcgcccat
      481 tgcgcgccga gcaggaacgt gagcagcagc agcgtgggcg cggtcagcgc tccgagcgcg
      541 gtcgcgaacg ccagcagtcc ggcgatgacc gcgccggccg cctgcgtgcc gatcaggatc
      601 gcgcgccggt cggcgatgtc cgcgagcgcg ccggcgccga tcgcgagcag gaacgacggc
      661 gccgtgatcg ccacctgcac cagcgccacc agcagcggtg agggagtgag gttggtcatc
      721 tcccacccgg cggccacgtt ctgcagccag gtgccgatgt tggaaccgag gctggcgatc
      781 cacagcgcgc ggaacaccgg ctcggcgagc ggtgcccacg cgccggtatg ccggcggcgg
      841 gtggcgggcg gagggcggga cgacacggag aaatgcgcag cctgaaggcg ggacggaagg
      901 tacccgaccc gcgccgccgc gatggtacct gccttgacaa tcattgccta ggcaggaaaa
      961 tcgtcggcct gtcgcccgtt gccgatcccg tcatgccgag aaccacgccc cagccaccgc
     1021 gccgcgcgcg gcgccaatat cgcggcgacc gctaccggat cgaggagtcg atcggctacc
     1081 tgatgaagct cgcgctgtcg gcgctgcggc ggcggctcga ccgccggatg gccgagcacg
     1141 acctgaccgg cgtgcagatg ctgccgctgt tcctgatcgg tcgtggcgtg tgcggaaccg
     1201 cggccgagct ggcgcggcgg aacgacatcg atgccggcgc caccacgcgc ctgctcgacc
     1261 gcctcgaagc caaggggctg ctccggcgtt cccgcagcgc gcaggaccgg cgcgtggtgc
     1321 agctcgagct gaccgccgcc ggccgtgcga tcgccgacca gatcccgtac gcgctcgccg
     1381 acacgctgaa cgaggcgctg cgcgacttca gcgcgaccga ggtcgagacg ctgcgctcca
     1441 tgctccgccg catcgttgcc aacacggagg gcgcggcatg aagcgacgcg ccaccctgct
     1501 cgcgaccgca ctgctgctcg ccggctgcgc gagcttcgac ggcatcgcac cgggagtggc
     1561 acaacgcacc gcggcgcagc tcggcgcgac cccgtccgac gccgcatggc cgcgcgacga
     1621 ctggtggacg tggttcggag atcgtcaact cgacacgctc atcgagcgtg cgctggcggg
     1681 caatccgcag ctcgacgtcg cgcgcgcgcg gctggcgcgc gcgcaggcgg cagtggcggc
     1741 atccggggcg gcgcagaagc cgcacctcga tgcgcaggcg cagtcgacct ggcagcgctt
     1801 caacgaaaac ttcctctacc cgccgccgct cgcagggagc acggacagca gcaacacgct
     1861 gcagctcacg ggcagctggg aactcgactt tttcggtcgc catcgcgccg cactgcgcgc
     1921 cgcgctgtcg cagagccgcg cggtcgcggc cgaggcccag gccgcgcgcc tgctgatcac
     1981 cgcgtcggtc gcgcgcacct acatgcagct tgcacgcctg gtcgagacgc gcgacgtgct
     2041 cgccgccacg ctcgcgcagc gcgagcagat cgtgcggctg gtgcaggcgc gcgtgaagag
     2101 cggcctcgac acgcaggtcg agctgcgcca ggccgaagcg gccgtgcccg aaatccggca
     2161 gcaacgcgag gcgctcgacg aacagatgca gatcgcacgc cacgcgctgg cggcgctgct
     2221 cggcgccgga ccggaggcga cgcaggcgct cgcaccgcgc ctggcgcagg cgcaaccgcc
     2281 gccgctgccg gcgacgatcc cggccgagct gatgggccgt cgtgccgacc tcagcgcggc
     2341 gcgctggcga gtcgaggcgg ctgccgccga cgtcgacgcc gcgaaggcgc agttctatcc
     2401 gaacgtcaac ctgctcgcat tcgccggtct ccagagcctt ggctttgcgc actggctcga
     2461 cgccggcagc cgcacgttcg gcgccgggcc cgctgtgagc ctgccggtgt tcgatgcggg
     2521 tcgactgcgc gccgccctgc gcggccggac cgccgacttc gacgccgccg tggctgacta
     2581 caacgcggca ctgaccgacg ccgtgcgcga cgtggcggac cagatcgcat cgctgcagtc
     2641 gctcgagcgc caagccgccg aacagcgcgc cgcgcaaacg gccaccgagg gcgcgtacga
     2701 actcgcgctg ctgcgctatc gcgccgggct cggctcgtat ctcgcggtgc tcgtcgccga
     2761 aaccagcgtg ctcgcgcagc ggcgcatcgg tacggatctg aaggcgcgcc gcgtcgacgc
     2821 ctcgatcgag ctgatgcgcg cactcggcgg cggctatcga gccgacgccg accgtgagac
     2881 ccgcggcggc gatccggccg accccgcaac cagcgatcgc ggcgacgccg acgccgcgct
     2941 ggccgcgcgc accaacctgc ccgagccacg atgagcgcga ccgaccccga caagtcgaac
     3001 acccgtccgc gccgcgttgc gctcgctgcg ctcgcgggaa ccttcggtct cgccgcgctc
     3061 ggctatggcg cgtactggta tctcttcgag cgcaacctgg agaccaccga caacgcctac
     3121 gtggccggca atctggtgca gatcacgccg cagatcggcg gcacggtgct ggcgatccag
     3181 gccgacgaga ccgacttcgt gcgcgccggc cagccgctgg tgaatctcga tcgtgccgac
     3241 gcgcaggtcg cactggaaca ggcgcaggcg caactggcgc agaccgtgcg cgaggtgcgg
     3301 acgctgtacg cgaacaacgc atccctcgcg gcggtggtcg cgctgcgcga cgccgaggtc
     3361 gcaaaagcgc gcagcggcat cgccaaggcg caggacgacc tcgcgcgccg gcagtcgctg
     3421 gcggcgaccg gcgcagtgtc ggacgaggaa atcaagcacg ccgcgaccgc gctcgacgat
     3481 gcgaagagcg tgctcgccgc cgcacaggcc gccggcagcg cggcgcgcga acagctcgcc
     3541 gccagccagg cgctgaccgc aggcacgacc gtacacgatc acccgagcgt gcagcgcgcc
     3601 gccgcgcgcg tgcgcgaagc gcatctcgcg ctgtcgcgcg tcgaactgcc ggcgccggtc
     3661 gctggctacg tcgccaagcg cagcgtgcag gtgggcagcc gcgtccaggt cggcgcgccg
     3721 ctgatgacga tcgttccgct cgaccaggtg tgggtcgagg cgaacttcaa ggaagtccag
     3781 ttgcgcagcc tgcgcatcgg ccagccggcg cacgtgacgg cggacctgta cggcacgaag
     3841 gtcgagtacc acggcaccgt cgccggcctg ggcgtcggca ccggcagcgc cttcgcactg
     3901 ctgccggcgc agaacgcgac gggcaactgg atcaaggtcg tgcagcgcgt accggtgcgc
     3961 atcgcgctcg acacgcgcga actggccgcg catccgctgc gcgtcgggct gtcgatggaa
     4021 gtgacggtcg acgtggcgga ccgcagcggc aagctgctcg cggatgcgcc gcgcgcgcag
     4081 ccggtggcca cgaccgaatc gttcgcgccg ccgacgcgcg aggccgacgc gatgatcgaa
     4141 cggatcatcg ccgccaacct tgccggcgcg ccggcaccgc gcgcgcccgc cgaggtcgcg
     4201 gagcctgcgt cgagcaggcg ctgagcgcgg acaagcgcat ctcgatcgat gtcggccgca
     4261 gcacgttcgc acgcgcaccc gccgctcgcc ggcggcgcgc tcgtcatcgg cacgctcgcg
     4321 ctgtcgctgg cgaccttcat gaacgtgctc gacacgtcga tcgccaacgt gtcgatcccg
     4381 gcaatctcgg gcgacctcgg cgtggcgccg aaccagggca cgtgggtgat cacgtcgttc
     4441 ggcgtcgcca acgcgatctc gctgccgctg accggctggc tgacgcagcg cttcggccag
     4501 gtgcggctgt tctgcatcag cgtgctgctg ttcgtgctcg cgtcgttcct gtgcggactg
     4561 gcgccgaacc tcgagacgct gatcgcattc cgcgtgatgc agggctttgt cgccggaccg
     4621 atgattccgc tgtcgcaaac gctgctgctg gcaagttatc caccgcggcg ctccggcacc
     4681 gcgctggcgt tctggggcat gaccacgctg atcgcgccgg tcgtgggtcc gctggcgggc
     4741 ggctggatca ccgacaacat cggctggccg tggatcttct tcatcaacat cccggtcggg
     4801 ctggctgcga ccattgcgac ctggtcgatc tttcgccgtc gtgaaacgcc gacgcgcacc
     4861 gtaccgatcg acggcgtcgg gcttggcctg ctggtgctgt gggtcggctg cctgcaggtc
     4921 atgctcgaca agggtcggga gctggactgg ttcgcttcgg gccagatcgt ggcgctcgca
     4981 gtcaccgccg tcgtcgggtt cgccttcttc ctcgcatggg aattgaccga tgcgcatccg
     5041 gtcgtcgagg tgcgcctgtt cgcgcgccgc aacgtcacgt tcgccacgat cgcgatcgcg
     5101 gtcgcctacg cattgttctt cggcaacctc gtgctgctgc cgctgtggct gcagcagtgg
     5161 atgggttaca ccgccacgca ggccggctgg gcgctggcac cggtcggcgc tctggcgatc
     5221 gtgctgacac ccgtcgtcgg acgctatgtc aaccgcgtcg atccgcgccc gatcgtgacc
     5281 gcatcgttca tcaccttcgc gctggtgatg tacctgcgcg cgcagttcac caccgacacc
     5341 gacctggcga cgatcatggt gccgaccgtg atccagggcg cggcggtcgc ctgcttcttc
     5401 attccgctga cgtcgatttc gctgtcgggg ctgacgccgg accgaatcgc cagcgcatcg
     5461 gggctggtga acttcgcccg catcaccgcc ggctcgttcg gcacctcgat cgcgaccacg
     5521 ctgtgggacc accgcgcgtc gctgcaccac tcgcaactcg tcgagcacct gacgcgcggg
     5581 aatctcccga ccgaacgctt cctcgagtcc gcgacgcgcg ccgggctgac gcaggaacag
     5641 gcgctggccc tgctgaatcg cggcgtcgac acgcaatcgt tcacgctcgc ggccgacgag
     5701 atcttctacg tctcggcgtt actcttcctc gcgctggtgg gcctcgtatg gttgacccgg
     5761 ccccagcggg gcgggctggc gaacatcgat ggcggcgcac attgagccag cctcggttag
     5821 attggcggct cggcccgctc cgcatcgccc cgccgacaca gtgctcgacc gtgccgcctt
     5881 gatcgaccgc ctactctgat ggcccgcacc ttcctcgtgc tcgctgcgct cagcggattc
     5941 atcgccgtcg ccgccggcgc gttcggcgca cacgcgctga agacgcgcct ggcggccgac
     6001 ctgctggcgg tattcgacac ggcggtgcgc taccagatgt tccacgtcgt cggactgatc
     6061 gccgccgcat ggctcgcggc gcgcagcccg ggcccggcag tgctggcgag cggttggtgt
     6121 ttcgtcgccg gcaccgtact cttcagcggc agtctgtatg cgcttgcact gaccggcaca
     6181 cgctggctcg gcgcgatcac gccgttcggc ggcgttctgt tcctcgccgg ctggctgctg
     6241 ctggcgtgga cggcctggcg gctagactga gcgacgggag gatttgcgat ggccgcagcc
     6301 ggcgaaatcg aaaacatggc ggtgttctgc gacttcgaga acatcgcgct cggcgtgcag
     6361 gacgccaagt acgagaagtt cgacgtcaag gccgtgctcg acaagctgct ggtcaagggc
     6421 agcatcgtgg tcaagaaggc gtactgcgac tgggcgcgct acaaggagtt caaggcgccg
     6481 atgcacgagc aggcgttcga actgatcgag ataccgcacg tgcgccagag cggcaagaac
     6541 tcggccgaca tccgcatggt cgtcgacgcg ctcgacctgt gctacacgaa gtcgcacgtc
     6601 gacaccttcg tgatcgtcag cggcgactcg gacttctcgc cgctggtgag caagctgcgc
     6661 gaaaacaaca agaccgtgat cggcgtcggc gtgaaggcgt cgacctcgga cctgctgatc
     6721 aagaactgcg acgagttcat cttctacgac gacctcgtgc gtgccggcga ggcgaggcgc
     6781 cggcaggcgg cgaagaggcc gaagcgggtc gagccgaaga aggccgagca ccgaatggcg
     6841 ggcgagcccg ccgccaacga cgacgacgcc gcgcgccgcc gccaggccgc gatcgacctc
     6901 gtgctcgaga cgctggagga actgatcgag gcgcgcggct cggatgggcg catttacgcg
     6961 tcgatggtca agcaggcgct gaagcggcgc cgccccgagt tcaacgagag ctaccatggc
     7021 tacaagagct ttggcgcgct gatcgacgac gccgccacgc gcggcgtgct gacggtcgcc
     7081 aaggacgaga agaccggcca gcagctggtg cgcgtcgcag cccccgacca atgaacgaca
     7141 cctgccggga tccgcgcttc cggaatccgt agcgcgtcga tcaccggccg ttcccgcacg
     7201 agacttgccg caggcacggc ggcgacccgc ggtcgcgcac gattcggcgt tcacgctgac
     7261 gggcggcggg aaaccgctgg cagcgaggca ggcgccgagg attgccgacc gcaagcttcg
     7321 gatccggtca gcgctcggcg ccgccggcag cgaccgacgg cgcttcggta tcgtcgcgca
     7381 ccatgcccgt cgacggccgt cgccgcatca ctgcgtacct gttgctgacc gcgtcgatga
     7441 cgctggtcgg ttcctacgtg gcgctgtcca agcccctggt ggcggcgatc ccggtgttcg
     7501 cgctggcgtt ccttcgcttc ggcatcgcgg ccgtatcgat gttgccgtgg acgctgccac
     7561 gcccgcacga aacgccgctg acgcgacgcg agcatgggct gttcttcctg atgtcgttcc
     7621 tcggcaattt cctgttctcg atctgcatgc tgagcggtgt ggcgctcacc accgccaccg
     7681 cggcgggcgt gatcatggca accattccgg cggtcgtggc ggcgggctcg tggctgttcc
     7741 tgcgcgagcg actgacgccg cgcgttctgg ccgcgatcgc gctggcgatc ggcggcatcg
     7801 cgctgttgca gttcgcgcgc gcatcggacg cgtcgcggca gacgacgctg ctcggcaatg
     7861 cgctgatctt cggcgccgtc gtgtgcgagg cgagctacgt gatcatcggc aagcggctcg
     7921 cggccacgcg tacgccgctg cgcgtctcgg cgctgatcaa tctgtgggga ctcgcgctga
     7981 tgacgccgtt cggcctgtgg caactggcgc gcttcgacct cggcgcggtg tcggtgccga
     8041 tctgggcgct gctcgtgttc tactcattgg cggccagcct gttcgcggtg tggctgtgga
     8101 tgacagggct gaagcacgtg ccggcccacc acgccggcgt gttcacggtc gcgctgccga
     8161 tcgcggccac cgcggtcggc gtggcgtttc tcggcgagca gttcacgctg ctgcatgggg
     8221 gcgcgctgct gcttgcaagc acgggcgtcg tgctgatcgc atctgcccgt cattaggctg
     8281 ggatcagacc ggcgtcgcca gcaccgcttc gcaggtcgcc cgctccggtg cgccggcggg
     8341 cagcttgccg cacaccgcgg caagttgcgc gcgcagccgc gccagcaccg cagcgtggct
     8401 gccgtccttg ttccactccc cgagcttggt cgagatgcgg tcgagcgccg cgcgattgcg
     8461 cccgtagaac gtctccggct tcggctcgat gtcggcgatc acgccggcga gtgcacgctc
     8521 gatgcgtgcg ccatcctgcg gcgccagatc gatcagcgcg cgcacgtagg tcgcgcccca
     8581 ttgcagccgc gtcgccggcc cctgcgacgt cgcgtaggcc tgctcgtacc agttcaatgc
     8641 cgtcgccgga tcgccgcgct tgcgcgcgtt gctggccagc ccgagcatca ggtagtacgg
     8701 cgcggctgag cgggccagcg ctgcctgcag cagcgcgtcg gattcggcga gcagatcagc
     8761 atcggccagc aggtcggccg acgcggtgac gaccgcctcg cgttcgaagc ggtcggtcgc
     8821 gtcgcggttc gcgcgcagca ccacgccacg cacttccgtc tgcagcgcca ttggcagcgc
     8881 acccttgggc acgtcgagcc gcgccagcac cacccgcgcg tccgccgccg cgatgcgatc
     8941 gagccgcgcc agcccggcgt catcgcagaa caacgccagg cgccgatccc acatgcgctg
     9001 caatttcgcg cgcgccggcg tgttcgcgtc cgtcaggtaa ccgacgatgt cctgcgcgcc
     9061 gtacgcgaac tcgacgaaat tcgcccgcgc gagatcatcg tcgcccagcg tcttctcgat
     9121 gcgcgccagc gcgacgcgct tgtcgatgcc ggacgcatcc ttgtccttcg cgtccgccgc
     9181 cagcgacacc gccttcagcg tgaggcgcgc cgccgcgtcc ggcgctttcg cggcgccagc
     9241 ctgtgcgagc ttccacagca ccggcgccag ttcacgcttc ggcaccagcg cctggtgatc
     9301 ggtctcccac gaatagaacg cgagcagccg ccagtcgttt gccgccagcg cggccggacg
     9361 cgtctgcgcg ctggcaagca gcgcccgaac cgggcggccg gcattcatgc cgagcgccag
     9421 cacctgcatg tagcgcgcgc catcgacctc gcccggcaac cgcgtgatct cggtaccgtc
     9481 ggggcggaac aggatcatcg tcggataggc accgaccttg aagcggctgg cgatcttctg
     9541 cgcgttcggc gcgtcgccgt ccagatacac cggcacgaaa aagcgcgagc gctcgatgaa
     9601 gtcacggcgc ggaaagatcg tcgccttgac ctggttgcag ggtgggcacc agaccgcgcc
     9661 ccagtagaga aacagcggct tgccctcgcg cttcgcctgg gcgaacgcgg cgtcgacgtc
     9721 gccgtcgtgc caggcgatcg cgtcgccggc aacggccggc gacgcgatga agaacagcag
     9781 ccagatcagt ttccggagca ttgtggcggc cagtctaatc cgccagcgcc cgctcgatca
     9841 gcggcagcgc cagcgcgcgc gcgtcgaccc gcgcaccgat gcgcgccagc agcaggaacg
     9901 agccgaccag cttgcggtgc aggaacatcg tctccggcgg cggcgcacgc agcaaccctc
     9961 gccgcagccc cagatcgatg ccaagcgcgc gcccccgcgc cggcaggtcg ctggccgcga
    10021 agtcgtacgg accgcgatgc cgcagcggct cgcagacgag cctgatgaca tcgaccgcgg
    10081 cctgcacgcg ctcgaccggg tcatcggcgg tcacgtagcc gatcgccacc gcgtaatgcg
    10141 cgatggcgtc ggcgtcctcc tcgatcactg cgcgcgtgat gcgcgcatag ttggcgacga
    10201 accgcgcatc gaacgcgcgc gtggcgccga agtcgagcag caccacgcgc ccgcccggct
    10261 gcaccaggta gttggcgaag ttcgggtcgg tctgcatcac gcggaactcg aacagctcgc
    10321 gaaagagcag ccgctccagc agcgtgccaa ccgcgtcgcg ctgcgctggc ggcacgtcgg
    10381 cggcgagcga ttccagcggg gcgccctcga catagtccat cgccatcacg cgcgtggtcg
    10441 tgaagtcgcg atgcacgcgc ggcacgatca gcagcggctc gtcggcgacg agccgcgcgt
    10501 aatgctcgag gaacccggcc tcggcgcggt agtcggcctc ctgcagcaac tgccgcttcg
    10561 cctcggcgac gatgccgtcc acgtccacat cgaacggcag caggttgaaa agcttcagca
    10621 gcgctgcgac gttgtcgacg tcgctcgcga tcgagcgcgc cacgccggga tactgaatct
    10681 tcagtgccag ctcgcgcccg tcgcgcgcgc gcgc
//
LOCUS       DBNHBY010000731         2367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_528858, whole genome shotgun sequence.
ACCESSION   DBNHBY010000731 DBNHBY010000000
VERSION     DBNHBY010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_528858"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /gene="aceE"
                     /locus_tag="AC16HB_17245"
     CDS             <1..1743
                     /gene="aceE"
                     /locus_tag="AC16HB_17245"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR5608590.1"
                     /translation="TVDGEYQNYKANDGAFVRKNFFGKFPQTAEMVSRMSDADIWRLN
                     RGGHDPHKIYAAFAAAVKTEGLPSVILAKTVKGYGMGKIGEGKNPTHQVKKLDTDAVR
                     EFRDRFSIPIPDDKVEDVPFFKPPENSPEMRYLHERRHALGGYLPQRRRRADEKLAAP
                     PLDAFAAVLEPTAEGREISTTQAFVRILTQLVRDKTIGPRVVPIVADEARTFGMEGMF
                     RQLGIFSQQGQLYEPVDKDQVMYYREDKKGQILEEGINEAGAMADWIAAASSYSNSNR
                     IVVPFYIFYSMFGFQRVGDLSWLAGDIQARGFLLGATSGRTTLNGEGLQHQDGHSHLI
                     SSTIPNCVSYDPTFAHELAVIIRDGMRRMVEEQENVFYYVTVMNENYPQPGLKRGQEE
                     GILKGMYRFREGPAGSGMGMSIPRVQLLGCGTILREVIAASELLERDWGVAGDVWSVT
                     SFTELRREGMDCERWNLLHPAEPPRKAFVTQQFERTVGPLIASTDYMRTFADQIRPYL
                     PKGRTYTVLGTDGFGRSDTREKLREHFEVDRHFVTIAALKALADDGTLPIAKVAEALR
                     KYGISPDKTNPTHA"
     gene            1858..>2367
                     /locus_tag="AC16HB_17250"
     CDS             1858..>2367
                     /locus_tag="AC16HB_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5608591.1"
                     /translation="MGIEIKVPDIGDFKDVEIIEVLVRPGDAVAKEQSLITVESDKAS
                     MEIPSSAAGVVKEMRVGLGDKVSEGSVILVLEPSEATARQAAAPAPAPVPAPAAVAPA
                     PAPAPVAAAGGGVIEVKVPDIGDFKDVEVIEVLVKAGDVVAKEQSLITVESDKASMEI
                     PSSAAGTVHE"
BASE COUNT          451 a          750 c          829 g          337 t
ORIGIN      
        1 accgtcgacg gcgagtacca gaactacaag gccaacgacg gcgcgtttgt gcgcaagaac
       61 ttcttcggca agttcccgca gaccgcggag atggtgtcgc gcatgtcgga tgccgacatc
      121 tggcggctga accgcggcgg ccacgacccg cacaagatct acgctgcgtt tgccgcggcc
      181 gtgaagaccg agggcctgcc aagcgtgatc ctcgccaaga cggtcaaggg ctacggcatg
      241 ggcaagatcg gcgaaggcaa gaacccgacg caccaggtca agaagctcga cacggacgcg
      301 gtgcgcgaat tccgcgaccg cttcagcatt ccgatcccgg acgacaaggt cgaggatgtg
      361 ccgttcttca agccgccgga gaactcgccc gagatgcgct acctgcacga gcggcgccac
      421 gcgctcggcg gctatctgcc gcagcggcgc cgccgcgcgg acgagaagct cgccgcgccc
      481 ccgctcgacg cgttcgccgc ggtgctggag ccgactgccg aagggcggga gatcagcacc
      541 acgcaggctt tcgtgcgcat cctcacgcaa ctggtgcgcg acaagacgat cggcccgcgc
      601 gtggtgccga tcgtcgccga cgaagcgcgc actttcggca tggaaggcat gttccgccag
      661 ctcggcatct tcagccagca gggtcagctg tacgagccgg tcgacaagga ccaggtgatg
      721 tactaccgcg aggacaagaa gggccagatc ctcgaggaag gcatcaacga ggcgggcgcg
      781 atggcggact ggattgccgc cgcaagctcg tactcgaaca gcaatcgcat cgtggtgccc
      841 ttctacatct tctattcgat gttcgggttc cagcgcgtgg gcgatctgtc atggctggcg
      901 ggcgacatcc aggcgcgcgg cttcctgctc ggcgcgacca gcggccgcac cacgctgaac
      961 ggcgaggggc tgcagcacca ggacggccat tcgcacctga tcagcagcac gatccccaac
     1021 tgcgtgagct acgacccgac cttcgcgcac gagctggcgg tgatcatccg cgacggcatg
     1081 cggcggatgg tggaagagca ggaaaacgtc ttctactacg tcaccgtgat gaacgagaac
     1141 tacccgcagc cgggtctcaa gcgcggccag gaggagggca ttctcaaggg gatgtaccgg
     1201 ttccgcgaag gcccggccgg cagcggcatg ggcatgtcga ttccgcgcgt gcagctgctc
     1261 ggctgcggca cgatcctgcg cgaggtgatc gccgcgagcg aactgctcga gcgcgactgg
     1321 ggcgtggccg gtgacgtatg gagcgtcacc agcttcaccg agctgcggcg cgagggcatg
     1381 gactgcgagc gctggaacct tctgcatccg gccgagccgc cgcgcaaggc gttcgtgacg
     1441 cagcagttcg agcgcaccgt cggcccgctc atcgcgtcga ccgactacat gcgcacgttc
     1501 gccgatcaga tccggccgta cctgcccaag ggccgcacct acaccgtgct cggtaccgac
     1561 gggttcgggc ggtcggacac gcgcgagaag ctgcgcgagc acttcgaggt ggaccgccac
     1621 ttcgtcacga tcgcggcgct gaaggccctg gccgacgatg gcaccttgcc gatcgcgaag
     1681 gtcgccgagg cgctccgcaa gtacggcatc agtcccgaca agacgaaccc gacccacgcg
     1741 tgagcgcggg ggaaggcgtg accgcgcgag cggaccatga acgagctgat caagcgcggg
     1801 atgcggcgca ccgacgtgct gctgacgctg cccgaactcg aacataaggc cgaacaaatg
     1861 ggaatcgaga tcaaggtgcc ggacatcggc gacttcaagg acgtcgagat catcgaggtg
     1921 ctggtgaggc ccggcgacgc ggtggcgaag gagcagtcgc tgatcaccgt cgagagcgac
     1981 aaggcgtcga tggagatccc gtcgtcggcc gccggtgtcg tgaaggagat gcgggtcggt
     2041 ctcggcgaca aggtgtccga aggatcggtg atcctggtgc tggaaccatc cgaagcgaca
     2101 gcaaggcagg ccgcggcgcc cgcgccggcg cccgtgccgg cacctgcagc ggttgcgccg
     2161 gcgcctgcgc ccgcaccggt cgcggcagcc ggcggcggcg tcatcgaggt gaaggtgccc
     2221 gatatcggcg acttcaagga cgtcgaagtt atcgaagtgc tggtgaaggc cggcgacgtg
     2281 gtggcgaagg agcagtcgct gatcacggtg gagagcgaca aggcgtcgat ggagattccg
     2341 tcgagcgcgg cgggcacggt gcatgag
//
LOCUS       DBNHBY010000732         3877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_663584, whole genome shotgun sequence.
ACCESSION   DBNHBY010000732 DBNHBY010000000
VERSION     DBNHBY010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_663584"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16HB_17255"
     CDS             complement(<1..533)
                     /locus_tag="AC16HB_17255"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5608592.1"
                     /translation="MALVAMRQLLDHAAENGYGLPAFNVNNLEQVQAIMEAASEVNAP
                     VIMQASAGARKYAGENFLKHLIIAAVESFPAIPIAMHQDHGQSPAVCEGAIRLGFSSV
                     MMDGSLREDGKTVSDYDYNVQVTKKVVDLAHAVGVSVEGELGCLGSLETMRGDKEDGH
                     GAEITMTKEMLLTDPDQA"
     gene            complement(642..2105)
                     /locus_tag="AC16HB_17260"
     CDS             complement(642..2105)
                     /locus_tag="AC16HB_17260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5608593.1"
                     /translation="MTDDDGRLARTHRRGAFIARAARVIRLSSCVGLIAALAACAQLP
                     TRPTFGPIADVPATVAAPATTALSAAYASAGARRETALRVWKLIEERFYDPKFNGVDW
                     VGARDRYVPRAEAARSDAEFYAALKAMVATLGDSHTLVLTPRETVDRRQFVTPRLGFD
                     LGWIERRVAIVEVESGSPAAAAGLRAGDVLRAVNGTRFDDDFLRQARSSPQPAESPAA
                     EAIDAAADARQRGDNERERVLRAAARVVRAAVGAPPALKTVHVEVERDAGVAESTFTA
                     TLAPRPPSADFRRLDGDVALIRFTRFLPEVRPAVEQALLGAQRAPAIIIDLRGNSGGL
                     FDFYRWFVGRFLAAPRTVMQALRREPASAVQGSGDVRAGGGVDALVQPLAVLVDARTA
                     SAAELTAVTLAEQRGALLVGDETCGCAVGVRAEYVLPDGGGLRISETGFVSARGARME
                     GRPTAPTIRVEPTLADLRDGRDVALEEAQRRLQSNLH"
     gene            complement(2254..3690)
                     /gene="pyk"
                     /locus_tag="AC16HB_17265"
     CDS             complement(2254..3690)
                     /gene="pyk"
                     /locus_tag="AC16HB_17265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5608594.1"
                     /translation="MPRSTKIVATLGPASSDPQVLRRLVVAGVDVVRFNFSHGKPEDH
                     LARAQAVRNAASACGREIAIMGDLQGPKIRIGRFENGKVTLAAGAGFVLDADCQVGTA
                     ERVGLDYKDLTRDVKPGDVLLLADGLIVLDVERVTGSEIHTVVRLGGELSNNKGINKQ
                     GGGLSAPALTAKDVRDSETSMECGADFVAVSFVKNRTDVEMARRLAVIAGERHGIKPQ
                     IIAKIERAEAIPVLQEIVDAADGIMVARGDLAVEVGNAAVPALQKRMIRMARAANKLA
                     ITATQMMESMIVNAVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPVEVVEAMAAIV
                     EEADRSASVMLDTQFLDRTFARVDQSIAYGSLFTAYHLRCAAICALTESGSVTLWMSR
                     HDINVPIYALTQSVSTQRRLALYRNVQPLHLPMSSDRDATLAQAERRLLDGGYVKRGD
                     LIVMTVGEPMGQPGGTNTLKIVRVGEHV"
     gene            complement(3695..>3877)
                     /gene="pgk"
                     /locus_tag="AC16HB_17270"
     CDS             complement(3695..>3877)
                     /gene="pgk"
                     /locus_tag="AC16HB_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5608595.1"
                     /translation="SATARGAFSLAGGGDTLAAIAKYGITDRVSYISTGGGAFLEFLE
                     GKTLPAIEVLEQRARG"
BASE COUNT          578 a         1381 c         1307 g          611 t
ORIGIN      
        1 gcctggtcgg gatcggtcag cagcatctcc ttcgtcatcg tgatctcggc gccgtggccg
       61 tcttccttgt cgccgcgcat cgtctcgagc gaaccgaggc agcccagttc gccctcgacc
      121 gacacgccga ccgcgtgcgc caggtcgacg accttcttcg tgacctgcac gttgtagtcg
      181 tagtcggaca ccgtcttgcc gtcctcgcgc agcgagccat ccatcatcac cgacgagaag
      241 ccgagcctga tcgcgccttc gcacaccgcc gggctctggc cgtggtcctg gtgcatcgcg
      301 atcgggatgg cgggaaagct ttcgaccgcg gcgatgatca ggtgcttgag aaagttctcg
      361 cccgcgtact tacgcgcgcc ggccgaagcc tgcatgatca ccggcgcatt gacctcggag
      421 gcggcttcca tgatcgcctg tacctgctcg aggttgttga cattgaacgc cggcaggccg
      481 tagccgtttt cggcggcgtg atccagaagc tgacgcatcg cgacgagtgc catgacgaac
      541 ctccagttgg agtcggggga acgagattga gactgcttgc ggcgattcta gtggcccggt
      601 cgatgccagc cgcggcagcc acacccgcgc gctgacctgg gtcagtgcag gttcgactgc
      661 aggcggcgct gcgcctcctc gagtgcgaca tcgcgcccat cgcgcagatc cgcgagtgtc
      721 ggctcaacgc ggatcgtggg cgcggtcggg cgcccctcca tccgcgcacc gcgcgccgac
      781 acgaagcccg tttcgctgat gcgcagaccg ccgccgtcgg gcaagacata ctcggcccga
      841 acgccaaccg cgcagccgca ggtttcgtcg ccgacgagca gcgcgccgcg ctgctcggcc
      901 agcgtcactg cggtgagttc cgctgcgctg gcggtccgtg cgtcgacgag cacggccagt
      961 ggctgcacca gcgcgtccac gccgccgccg gcacgcacgt cgccgctgcc ctgcacggcc
     1021 gatgccggct cgcgccgcag cgcttgcatc acggtgcgcg gcgccgccag aaaacggccg
     1081 acgaaccagc gatagaagtc gaacagtccg ccgctgttgc cgcgcaggtc gatgatgatc
     1141 gcgggcgcgc gctgggcgcc gagcagcgcc tgctcgacgg caggtcgcac ctcgggcagg
     1201 aaacgcgtga agcggatcag cgccacatcg ccgtcgaggc gccggaaatc cgcgctgggc
     1261 ggccgcggcg caagcgtggc agtaaacgtc gattcggcga cgccggcgtc gcgttcgacc
     1321 tcgacatgca ccgttttgag cgccggcggc gcgccaaccg cggcgcgcac gacgcgcgcg
     1381 gccgcgcgca gcacgcgctc acgttcgttg tcgccgcgtt gccgcgcgtc ggcggcggca
     1441 tcgatcgctt cggcggcggg cgattcggcc ggctgcggtg aactgcgcgc ctggcgcagg
     1501 aagtcatcgt cgaagcgcgt gccgttgaca gcgcggagca cgtcgccggc gcgcaggcca
     1561 gcggcggcgg cgggcgaacc ggactcgacc tcgacgatcg ccacgcgccg ctcgatccat
     1621 ccgaggtcga agccgaggcg cggcgtgacg aactggcggc gatccacggt ttcgcgcggc
     1681 gtcagcacca gcgtatgcga gtcgcccagt gttgcgacca ttgccttcag cgcagcgtag
     1741 aactcggcgt cactgcgcgc ggcttcggcg cgcggcacgt aacggtcgcg cgcgcccacc
     1801 cagtcgacgc cgttgaactt cgggtcgtag aagcgctcct cgatcagctt ccacacgcgc
     1861 agcgcggttt cgcgccgcgc gccggccgag gcataggcgg ccgacagcgc cgtcgtcgcc
     1921 ggcgcggcaa cggtggcggg tacgtcggcg atcggcccga acgtcggccg agtcggcaac
     1981 tgtgcgcagg ccgcgagcgc cgcgatcaac ccgacgcacg aggaaaggcg gatgacgcgt
     2041 gctgcgcgcg ctatgaatgc gccgcgtcgg tgcgtccgcg ccagtcgccc gtcatcatcg
     2101 gtcatacgca cgcagcagcg tcattccgca tcgtcaagcg tgcgcgccag ggcgcacgat
     2161 cctggcgccg gccgctcgac gcgctatgtc gagcttcacg cgcactgcgc acgcatgagc
     2221 cgcgccgaaa tcgtgccgat tgcgcatgac aacctacaca tgctctccaa cccgcacgat
     2281 cttcagcgtg ttggtgccgc cgggctgtcc catgggctcg ccgacggtca tcacgatcag
     2341 gtcgccgcgc ttgacgtagc cgccgtcgag cagcctgcgc tcggcctgtg cgagcgtcgc
     2401 atcgcgatcg ctcgacatcg gcaggtggag cggctgcacg tttcgataca gcgccagccg
     2461 gcgctgcgtg gacacgcttt gcgtgagcgc atagatcggc acgttgatgt cgtgccggct
     2521 catccacagc gtcaccgaac ccgactcggt cagcgcgcag atcgccgcgc agcgcagatg
     2581 gtatgcggtg aagagcgacc cgtaggcgat cgactggtcg acgcgcgcga acgtccggtc
     2641 gaggaactgc gtatcgagca tgaccgacgc ggagcggtcg gcctcctcga cgatcgcggc
     2701 catcgcctcg accacttcga ccgggtactt gccggccgcc gtctcggccg acagcatcac
     2761 cgcgtcggtg ccgtccagca ccgcgttggc gacgtcgctc acctcggcgc gcgtcggcac
     2821 ggcgttgacg atcatcgact ccatcatctg cgtcgcagtg atcgcgagct tgttggcggc
     2881 gcgtgccatc ctgatcatgc gcttctgcag cgccggcacc gcggcgttgc cgacttcgac
     2941 cgccaggtcg ccgcgcgcga ccatgatgcc gtcggccgcg tccacgatct cctgcagcac
     3001 cgggatcgcc tcggcgcgct cgatcttggc gatgatctgc ggcttgatgc cgtgccgttc
     3061 gccggcgatc accgccagcc ggcgcgccat ctcgacatcg gtgcgattct tcacgaacga
     3121 cacggcgacg aaatcggcgc cgcattccat cgaggtttcg ctgtcccgca cgtccttggc
     3181 ggtaagcgcc ggcgccgaca gcccgccgcc ctgtttgttg atgcccttgt tgttcgacag
     3241 ttcgccgcca agacgcacga ccgtgtggat ctcgctgccg gtgacgcgct cgacgtcgag
     3301 cacgatcagg ccgtcggcga gcagcagaac gtcgcccggc ttcacgtcgc gcgtcagatc
     3361 cttgtagtcg aggccgacgc gctctgcagt gccgacctgg cagtcggcgt cgagcacgaa
     3421 gccggccccc gcggccaggg tgaccttgcc gttctcgaag cggccgatgc ggatcttcgg
     3481 cccctgcaga tcgcccatga tcgcgatctc gcggccgcaa gcgctcgccg cgttgcggac
     3541 cgcttgcgcg cgcgccagat ggtcctccgg cttgccgtgc gagaagttga agcgcaccac
     3601 gtcgacgccg gccaccacca gccgccgcag cacttgcgga tcactcgacg cggggccgag
     3661 cgtggcgacg atcttcgtgc tgcgcggcac gtgctcagcc cctggcgcgc tgttccagga
     3721 cctcgatcgc cggcagcgtc ttgccctcga gaaactcgag gaacgcaccg ccgccggtgg
     3781 agatatagct gacgcgatcg gtgatgccgt acttggcgat cgcggcgagg gtgtctccgc
     3841 cgccggcgag cgagaacgcg ccgcgcgcgg tggcact
//
LOCUS       DBNHBY010000733         2956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_597054, whole genome shotgun sequence.
ACCESSION   DBNHBY010000733 DBNHBY010000000
VERSION     DBNHBY010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_597054"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..749
                     /locus_tag="AC16HB_17275"
     CDS             69..749
                     /locus_tag="AC16HB_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR5608596.1"
                     /translation="MLVDEPARAGDPWGGALAVTSDYVFRGVSQRDGAAAIQGDLHYR
                     SQRGWFAGLWASTADPPPGYVADYELNFYAGREWGWSERWSTALSFVRYQYPNAPARG
                     RYDYNEVSASVRFEDRLALTIAFSPDAWRYSHYYGWRDRRRTISYEASLRQPAIPPLT
                     VIAAIGYYDTTALFGESYWAWNAGLAAMVGPVEITLLRFGVDASGRRLFGADAADGRW
                     VVSAAWRF"
     gene            827..1417
                     /locus_tag="AC16HB_17280"
     CDS             827..1417
                     /locus_tag="AC16HB_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5608597.1"
                     /translation="MSPAEQHGRATAAGADAGREDARDAALLHRIAASDRAALEALYT
                     LYHRRLARFLVRFTSRYELAEEIINDTMWIVWQQAAAFRGAARVSTWIMGIAYRCALA
                     TLRHVSVRPGVVDDDAPLESADPATEFEQRELLDRALALLPLEQRMVLELTYILGHSC
                     EEIAAITDCPVNTVKTRMFHARRKMRQLLPGLSGQQ"
     gene            1428..2102
                     /locus_tag="AC16HB_17285"
     CDS             1428..2102
                     /locus_tag="AC16HB_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5608598.1"
                     /translation="MRREPDTLTCNDESARLLPWYVNGTLTEADAARVAAHLERCASC
                     RAEAAREEHTRTLLRIPAQVEHTPQAGLRKLLARIDASEPAAAAPGVPAALLPRATRG
                     PMRWLAAAVIVQAAALAAIAGATLSGAKFDTAPRFQTLTSTAPEGAHIRILFTPAMTL
                     AELQELLHANQLTAVGGPTEAGLFTVALRPSLVRPDEQDAVLARLRADPRVRFAELVA
                     PDAKAR"
     gene            2111..>2956
                     /locus_tag="AC16HB_17290"
     CDS             2111..>2956
                     /locus_tag="AC16HB_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5608599.1"
                     /translation="MALWLLALALAGCAGMTAVAPAARLPIDARAHPERYVVVTVNNA
                     PAAVSTYAASTPRGYGGQGPYRAGADAIALARRIAADHRLQEVAAWPIELLSLHCLVY
                     ELPEGAERDAVLTALRRDQGVESAEPLSLFTLRASAYNDPYAPLQRNLDELGIATAQQ
                     WSRGDGVRIAIIDTGVDTTHPDFAGRLEALRDLVGDGGAPAEGHGTAVAGIIAAMPNN
                     GMGIAGIAPDARVLPLRACWSVGSTTGACNSFTLAQALVAALDAHASIVNLSLGGPGD
                     PLLERI"
BASE COUNT          431 a         1046 c         1028 g          451 t
ORIGIN      
        1 ccgcaggacg acactcgagc cgccgtgcac gacgatccag cgtcgccctg atctggattg
       61 cggcgctcgt gctcgtcgac gagccggccc gcgccggcga tccgtggggc ggcgcactcg
      121 cggtgacgag cgactacgtc tttcgcggcg tatcgcagcg ggacggcgcg gcggccattc
      181 agggcgatct gcattaccgc tcgcagcgcg gttggttcgc cgggctctgg gcgtcgacgg
      241 ccgacccgcc gccgggctat gtcgccgact acgaactgaa cttctatgcc ggccgcgagt
      301 gggggtggtc ggagcgctgg agcacggcgc tcagcttcgt gcgttaccag tacccgaatg
      361 cgcccgcgcg cgggcgctac gactacaacg aggtgtccgc gtccgttcgc ttcgaggatc
      421 ggctcgcgct gacgatcgcg ttctcgcccg acgcgtggcg ctattcgcat tactacggat
      481 ggcgggatcg tcgccgcacg atcagctacg aggcgtcgct gcgtcagccg gccatcccgc
      541 cactcacagt catcgcggcg atcgggtact acgacaccac ggcgctgttc ggcgagtcat
      601 attgggcgtg gaatgcaggg ctcgcggcga tggtcggccc ggtggagatc accctgctcc
      661 gcttcggcgt cgacgcgtcg gggcgacgcc tgttcggcgc ggacgccgcc gacggccgct
      721 gggtggtctc ggccgcgtgg cgattctgac cggattgaac cggcgggccc tcccgcggca
      781 ctaacggcat caagaacagg tctgacttga atactggata cgcaacgtga gtcccgccga
      841 gcagcacggt cgcgctacag cagccggcgc ggacgcgggc cgcgaagacg cgcgtgacgc
      901 agccctgctc caccgcatcg ccgcatccga ccgcgccgcg ctggaagcgc tgtatacgct
      961 ctatcaccgg cggctcgcgc ggttcctggt ccgcttcaca tcccggtacg agctggccga
     1021 ggagatcatc aatgacacga tgtggatcgt gtggcagcag gcggccgcgt ttcgcggcgc
     1081 ggcgcgggtt tcgacgtgga tcatgggcat cgcctaccgc tgcgcgctcg ctacgctgcg
     1141 acacgtgagc gtccggcccg gcgtggtgga cgacgacgcg ccgctggagt cggccgatcc
     1201 ggccacggaa ttcgagcagc gcgaactgct ggaccgtgcg ctcgcgttgt tgccgctcga
     1261 gcagcgcatg gtgctcgagc tcacctatat cctcggtcat agttgcgaag agatcgccgc
     1321 catcaccgat tgcccggtga acaccgtcaa gacgcggatg ttccatgcgc gccgaaagat
     1381 gcggcaactg ctgcccgggc tgtcgggcca gcagtgagag acgaaacatg cgccgcgaac
     1441 ccgacacgct gacctgcaac gacgaatccg cccgtctgct gccgtggtac gtgaacggca
     1501 cgctgacgga ggcggacgcc gcgcgcgtcg ccgcgcacct cgaacgctgc gcgtcgtgcc
     1561 gcgccgaagc ggctcgcgag gagcacaccc gcacgctgct gcgcattccg gcacaggtcg
     1621 agcacacgcc gcaggcgggc ctgcgcaagc tgctggcgcg catcgatgca tcggagcctg
     1681 ccgccgcggc accgggcgta cctgcggcac tcctgccgcg cgcgacacgc ggcccgatgc
     1741 gctggctcgc cgccgcggtg atcgtccagg cggctgcgct cgccgccatc gccggtgcga
     1801 cgctctccgg cgcgaagttc gataccgcgc cgcgctttca aacgctgacc tccaccgcac
     1861 ccgagggcgc gcacatacgc atcctattca cgccggcgat gacgctggcc gaactgcagg
     1921 aactgctgca cgcgaatcag ttgacggcgg tcggcggccc cacggaggcg ggcctgttca
     1981 cggtcgcgct gcgcccgtcg ctcgtccggc ccgacgagca ggacgcggtg ctggcccggc
     2041 tgcgcgcgga cccgcgcgtg cgcttcgccg aactcgtcgc gccggacgcg aaggcgcgat
     2101 gaggttgcgc gtggcgcttt ggctgctggc gctcgcgctt gcgggctgcg ccggcatgac
     2161 ggcggtcgct cccgcggcgc ggcttcccat cgatgcgcgc gcgcatcccg agcgctacgt
     2221 cgtagtcacc gtgaacaacg cgcctgccgc cgtgagtacg tatgccgcgt cgacgccgcg
     2281 cggctacggc ggacaggggc cttaccgcgc cggcgcggac gcgatcgcgc ttgcgcgccg
     2341 catcgctgcc gaccatcggc tgcaggaagt cgcggcgtgg ccgatcgagt tgctcagcct
     2401 gcactgcctg gtctatgagc taccggaagg cgcagagcgt gacgcagttc ttaccgcgct
     2461 gcgtcgcgat cagggcgtcg aatcggccga accgctgtcg ctgttcacct tgcgcgcgag
     2521 cgcatacaac gacccctacg cgccgcttca gcgaaatctc gacgaactag gcatcgcgac
     2581 ggcacagcag tggtcgcgtg gcgatggcgt gcgcatcgcg atcatcgaca ctggcgtcga
     2641 cacgacgcat ccggatttcg ccggccgcct ggaggcgctg cgcgatctcg tgggcgacgg
     2701 cggcgcaccg gccgagggac atggcaccgc agtggccggc atcatcgccg ccatgccgaa
     2761 caacggcatg ggtatcgccg gcatcgcacc ggacgcgcgc gtgctgccgc tgcgggcctg
     2821 ctggtcggtc ggcagcacca cgggcgcgtg caactcgttc acgctcgcgc aggcgctggt
     2881 ggctgcgctc gacgcccacg catcgatcgt caacctgagc ctcggcggcc ccggtgaccc
     2941 gctgctcgag cggatc
//
LOCUS       DBNHBY010000734         2393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1746832, whole genome shotgun sequence.
ACCESSION   DBNHBY010000734 DBNHBY010000000
VERSION     DBNHBY010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1746832"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16HB_17295"
     CDS             complement(<1..287)
                     /locus_tag="AC16HB_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5608600.1"
                     /translation="MERRRVVVTGLGVVSPVGNDVPSAWSNLLAGKSGITRITKFDPS
                     TFRTQIAGEVKEFDITAYISAKEARRMDTFIHYGMAASIQAIRDSGLEITDA"
     gene            complement(323..565)
                     /gene="acpP"
                     /locus_tag="AC16HB_17300"
     CDS             complement(323..565)
                     /gene="acpP"
                     /locus_tag="AC16HB_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5608601.1"
                     /translation="MENIEQRVKKIVAEQLGVNEADIKNESAFVEDLGADSLDTVELV
                     MALEDEFETEIPDEEAEKIRTVQQAIDYVKAHSKKA"
     gene            complement(678..1448)
                     /gene="fabG"
                     /locus_tag="AC16HB_17305"
     CDS             complement(678..1448)
                     /gene="fabG"
                     /locus_tag="AC16HB_17305"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR5608602.1"
                     /translation="MSDLNVFAVNALNGEVALVTGASRGIGRAILGALARAGATVVGT
                     ATSEAGAASIAEHLTQLGAAGRGAALDVGDAAASDALVEKVVQDFGKLSLLVNNAGIT
                     RDTLALRMKDEDWAAVIDTNLSAVFRLSRAVIRGMMKAKHGRIVNITSVVGSSGNPGQ
                     ANYAAAKAGVAGMTRALAREVGSRGITVNCIAPGFIDTDMTRTLSEQQTQALLSQVPL
                     GRLGQADEIAAAVVFLASRAGAYVSGATLHVNGGMYMG"
     gene            complement(1445..2383)
                     /gene="fabD"
                     /locus_tag="AC16HB_17310"
     CDS             complement(1445..2383)
                     /gene="fabD"
                     /locus_tag="AC16HB_17310"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR5608603.1"
                     /translation="MKLAFVFPGQGSQSVGMLSSFAGDAAVEETLRRASVALGEDVAR
                     LIAEGPAETLALTVNTQPCMLAAGYAMYAAWRAAGGSAPELMAGHSLGEYTALTAAGV
                     LKFEDAVRLVRFRAQAMQDAVPVGTGSMAAILGLSDDLVRQACSEGAQGEVVEAVNFN
                     APLQVVIAGHTAAVERACEVAKRLGAKRAAMLAVSAPFHSSLLAPARERLRERLASVE
                     LSPPSVAVINNVDVASESDPARIRDAMARQAAAPVRWSEIIAKMAAEGATHVIECGPG
                     RVLAGLTRRAAPGVESFAISDAASLQDVLARVKPLT"
BASE COUNT          363 a          872 c          750 g          408 t
ORIGIN      
        1 gcatccgtga tttccaggcc cgagtcccgg atcgcctgga tcgacgcggc catcccgtag
       61 tggatgaagg tgtccatccg ccgcgcttcc ttggcggaga tgtacgcggt gatgtcgaac
      121 tccttgacct cgccggcaat ctgcgtgcgg aaggtcgaag ggtcgaactt cgtgatgcgc
      181 gtgatgcccg acttgccggc cagcagattg ctccaggcgc tggggacgtc gttgcccacg
      241 ggagacacga cgcccagccc ggtcacgacg acgcgtcggc gttccatctg cggtcggaag
      301 gcggtgcgag ttgcagtctg cgtcaggcct tcttcgaatg ggccttgacg tagtcgatgg
      361 cctgttgcac cgtgcgaatc ttctccgcct cctcgtccgg gatctcggtc tcgaactcat
      421 cctcgagcgc catcacgagt tcgacggtgt ccagcgagtc ggcacccagg tcttcgacga
      481 aggcggactc gttcttgatg tcggcctcgt tcacgcccag ttgctcggcg acgatcttct
      541 tcactcgctg ttcaatgttt tccatggacc cctcctcagg tgtctgtgtg ccgcgcgatc
      601 ctgccaatgc gattgcgagg tcggtcaaaa aagcgggcgc gattctatca gcccactacg
      661 cgtcatttcc ctgcgactca acccatgtac atgccgccgt tcacgtgcag cgtcgcgccc
      721 gagacatagg cgccggcgcg cgaagccagg aacacgaccg ctgccgcgat ttcgtccgct
      781 tgcccgagcc ggccgagcgg tacctgtgac agcagcgcct gcgtctgctg ctccgaaagc
      841 gtgcgcgtca tgtcggtgtc gatgaagccc ggcgcgatgc agttcacggt gatgccgcga
      901 ctgccgacct cgcgcgccag cgcgcgcgtc atgcccgcca cgccggcctt ggcggccgcg
      961 taattcgctt gtccgggatt gccggaactg ccgaccaccg atgtgatgtt gacgatgcgg
     1021 ccgtgcttgg ccttcatcat gccgcgtatc accgcgcgcg acagccggaa caccgccgac
     1081 agattcgtat cgatcaccgc ggcccagtcc tcgtccttca tccgcagcgc gagcgtgtcg
     1141 cgcgtgatac cggcattgtt caccagcaac gacagcttgc cgaagtcctg cacgaccttc
     1201 tccaccagcg cgtcgctcgc ggcggcgtcg cccacatcga gcgcggcacc gcggccggca
     1261 gcgccgagct gcgtcagatg ttcggcgatc gatgccgcac ccgcctcgct ggtcgcagtg
     1321 ccgacgaccg tggcgccggc gcgcgccagc gcgccgagga tcgcgcggcc gatgccgcgc
     1381 gacgcgccgg tgaccagcgc cacctcgccg ttcagcgcat tgaccgcgaa gacattcaga
     1441 tcgctcatgt cagtggcttg accctggcga gcacgtcctg caggctcgcg gcgtcgctga
     1501 tcgcgaagct ctcgacgccc ggcgccgcgc gccgggtcag gcctgcaagc accctgcccg
     1561 gcccgcattc gatcacgtgc gtcgcccctt cggcggccat cttcgcgatg atctcggacc
     1621 agcgcaccgg ggctgcggcc tggcgcgcca tcgcgtcgcg aatgcgcgcc gggtcggact
     1681 cggaggccac gtcgacgttg ttgatcaccg cgaccgacgg tggtgacagt tccacggacg
     1741 ccagccgctc gcgcaaccgc tcgcgcgccg gcgccagcag cgaagagtga aacggcgccg
     1801 acaccgccag catcgcggcg cgcttggcgc ccagccgctt tgccacttcg catgcgcgct
     1861 cgaccgccgc cgtgtgcccg gcaatcacca cctgcagcgg cgcgttgaag ttcaccgcct
     1921 cgaccacttc gccctgcgca ccctcgctgc acgcctgccg caccaggtcg tcggacaggc
     1981 cgaggatggc cgccatgctg ccggtgccga ccggcaccgc gtcctgcatc gcctgtgcgc
     2041 ggaagcgcac cagccggacc gcatcttcga acttcagcac gcccgccgcg gtcagcgcgg
     2101 tgtattcgcc caggctgtga ccggccatca gctcaggcgc ggacccgccg gccgcgcgcc
     2161 aggctgcgta catcgcatag cccgcagcca gcatgcacgg ctgcgtgttg accgtcagcg
     2221 ccagcgtctc ggccggccct tcggcgatca gccgcgcaac atcctcgccg agcgccaccg
     2281 aggcgcgacg cagcgtttcc tcgactgcgg catcgcccgc gaacgaggag agcatgccga
     2341 ccgattgcga tccctgtccg ggaaacacga acgcaagttt catcttgtca gta
//
LOCUS       DBNHBY010000735         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1341580, whole genome shotgun sequence.
ACCESSION   DBNHBY010000735 DBNHBY010000000
VERSION     DBNHBY010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1341580"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="AC16HB_17315"
     CDS             complement(<1..2944)
                     /locus_tag="AC16HB_17315"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5608604.1"
                     /translation="MAAPADVALRATALEDRYRVLDGRIFLSGVQALVRLLLEQRRRD
                     VAAGLNTAGFVSGYRGSPLGGLDLALWQAAQLLGEHHVRFEPGLNEELAATMVQGSQQ
                     VEALGGAQFDGVFGLWYAKNPGVDRAGDALKHANAAGTARHGGVLAVSGDDPGATSST
                     LPNQCDQAFIAALIPTLAPSSVAQIIEFGLAGWALSRYAGTWVGLKTVADVVEASATV
                     DVQRDSPRFERPAEFAQPLGIRWPDSRWDADARLLNERLPAAKAFVRLNRIDRAVFGA
                     ARPRIGIVTAGKALADVRGALHLLGIDAAAADVLGLAVFQVGMVWPLEPHALRDFADG
                     VEELIVVEERRAVIEPQLKELAYHWTASRRPAIVGKRDETGAPLLAETGILDPGRVAD
                     AIVARLLQRGLSADWRQRLSAIQPPAAPRAAGADVVRSPHFCAGCPHARSTRVPDGAL
                     AMAGIGCHSLRLGMPDAQTMFLVQMGGEGSNWIGAASFVRREHVFQNLGDGTYAHSGS
                     LAVRAAVAAGTRMTFRILYNGAVAMTGGQPAEGALTVAQIVRQLLDEGVRRVAVVSEV
                     PAHLEGTLPAGVALHGRDALSAVQRELAALPGVTALVYDQPCAIEKRRRRKRGLLPAP
                     AARPFINERVCEGCGDCVEQSQCAALLPVDTVLGRKRRIDASSCNVDLSCLDGLCPSF
                     VSIENARLRRAEPAALPAVELPPPAHAAARAAEIVVHGIGGSGVITVGAILGMAAHLD
                     GRGCTVLDNTGIARKGGAVASHVRIAADASTPLGSRIAAASATLVIAADLVVAAEASA
                     LTLIDTGRTRVLLNSDAVPTLNQRLQPDAPFDAGALRRRLTETAGDDAVTALPATALA
                     ERLLGDAVHANLLLLGAAFQIGRIPVSAAALERAIELNGEAVELNRRAFDIGRRAVLD
                     PQWVAQVLGGAAAPVELVGVDALIADRVRRLTDYHDARYAARFAALVQRTRAAQARAG
                     IADDALARA"
     gene            complement(2923..>3121)
                     /locus_tag="AC16HB_17320"
     CDS             complement(2923..>3121)
                     /locus_tag="AC16HB_17320"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5608605.1"
                     /translation="LLSYEIGFTTPPFGLLLFVMLGVAPRGTTLGTVARAALPYILCT
                     IGLVLLIVLFPQIALWLPRLM"
BASE COUNT          466 a         1133 c         1100 g          422 t
ORIGIN      
        1 ccgcgcgcgc gagcgcgtcg tctgcgatgc cagcgcgcgc ctgcgcggcg cgcgtccgct
       61 gcaccagcgc ggcgaagcgc gcggcgtagc gtgcatcgtg ataatcggtc aggcgccgca
      121 cgcggtcggc gatcagtgcg tcgacgccga caagctcgac cggcgccgca gcgccgccca
      181 gcacttgcgc gacccactgc ggatcgagca cggcgcgccg gccgatgtcg aatgcgcggc
      241 ggttcaattc caccgcttcg ccgttcaatt cgatcgcgcg ctcgagcgcg gctgcagaca
      301 cgggaattcg gccgatctga aaggcggcgc cgagcagcaa caggttggcg tggaccgcat
      361 cgccgagcag cctttccgcg agcgcagtcg ctggcagcgc ggtgaccgcg tcgtccccgg
      421 ccgtttccgt gagccggcgc cgcagcgcgc ccgcgtcgaa tggcgcatcg ggctgcagcc
      481 gctggttcag ggtcggcacc gcgtcggaat tcagcagcac gcgcgtgcgg cccgtgtcga
      541 tcagcgtcag cgccgacgct tcggccgcca cgacgagatc ggccgcaatc accagcgtcg
      601 cgctcgcagc ggcgatgcgg ctgcccagcg gcgtgcttgc gtcggccgcg atccgcacgt
      661 ggctcgccac cgcgccaccc ttgcgcgcga tgccggtgtt gtcgagcacc gtgcagccgc
      721 gcccgtcgag gtgggcggcc atgccaagga tcgcgcccac ggtgatcacg ccgctgccgc
      781 cgatgccgtg cacgacgatc tccgccgcgc gcgccgcggc gtgcgcgggc ggcggcaact
      841 ccaccgccgg tagagctgcc ggttctgccc ggcgcaggcg cgcgttctcg atgctgacga
      901 agctcgggca cagaccgtcg aggcacgaca ggtcgacgtt gcagctcgac gcgtcgatgc
      961 ggcgcttgcg tccgagcacg gtgtcgaccg gcagcagcgc cgcgcattgc gattgctcga
     1021 cgcagtcgcc gcagccttcg cacacgcgct cgttgatgaa aggccgcgcc gcgggtgccg
     1081 gcagcagtcc gcgcttgcgc cgccggcgct tttcgatcgc gcacggttgg tcgtaaacga
     1141 gcgcggtgac gcccggcagc gccgccagct cgcgctggac cgcggacagc gcgtcgcgcc
     1201 cgtgcagcgc cacgccggcg ggcaaggttc cttcgaggtg cgccggaact tcggagacaa
     1261 ccgcgacgcg gcgcacgcct tcgtcgagca gttgccgcac gatctgcgcg accgtgagcg
     1321 cgccttcggc cggctgaccg ccggtcatcg cgactgcgcc gttgtagagg atgcggaacg
     1381 tcatgcgcgt gccggccgcg accgcggcgc gcacggcgag cgatcccgaa tgcgcgtacg
     1441 tgccatcgcc gaggttctgg aacacgtgct cgcgccgcac gaaggacgcc gcgccgatcc
     1501 agttgctgcc ttcgccgccc atctgcacca ggaacatcgt ctgcgcatcg ggcatgccga
     1561 gccgcagcga gtggcagccg atgccggcca tcgccagcgc gccgtcgggc acgcgcgtgg
     1621 agcgcgcatg cgggcagccg gcgcagaaat gcgggctgcg tacaacgtcg gccccggcgg
     1681 cgcgcggcgc ggcgggaggt tgaattgcgc tcagcctttg tcgccaatcg gcagacagcc
     1741 cgcgttgcag cagccgcgcg acgattgcgt ccgcaacgcg ccccggatcg agtatgccgg
     1801 tctcggccaa cagcggcgcg ccggtttcgt cgcgcttgcc gacgatcgcg ggccggcgtg
     1861 acgcggtcca gtggtatgcg agctccttca gctgcggctc gatcaccgcg cgccgttcct
     1921 cgacgacgat cagctcctcg accccgtcgg caaagtcgcg cagcgcgtgc ggctccagtg
     1981 gccacaccat gccgacctgg aacaccgcga ggccaagtac gtcggccgcc gccgcatcga
     2041 tgccgagcag gtgcagcgcc ccgcgcacat cggcgagtgc ctttccggcg gtgacgatgc
     2101 cgatgcgcgg ccgcgcggcg ccgaacactg cgcggtcgat ccgattcaag cgcacgaacg
     2161 cctttgccgc cggcaggcgc tcgttcagca ggcgcgcgtc ggcgtcccag cgactgtcgg
     2221 gccagcgaat gccgagcggc tgcgcgaact cggccggtcg ctcgaaacgt ggggagtccc
     2281 gctgcacatc gacagtcgcg cttgcctcga ccacgtcggc gacggtcttc agaccgaccc
     2341 acgtgcccgc gtagcgcgac agtgcccagc cggcgagacc gaactcgatg atctgcgcca
     2401 cgctcgacgg cgccagcgtc ggaatcagcg ccgcaatgaa cgcctggtcg cactggttcg
     2461 gaagcgtcga cgaggtggcg ccgggatcgt cacccgacac cgcgagcacg ccgccgtggc
     2521 gtgcggtgcc ggcggcgttc gcgtgcttca gcgcatcgcc ggcgcgatcc acgcccggat
     2581 tcttcgcgta ccagagccca aacacgccgt cgaattgcgc gccgccgagc gcttcgacct
     2641 gctggctgcc ctggaccatc gtcgcggcca gttcctcgtt cagcccgggt tcgaaacgga
     2701 cgtgatgttc cccgagcagc tgggccgcct gccacagcgc gaggtccagg ccgcccaatg
     2761 gcgagccgcg atagcccgac acgaagccgg cggtgttcag tcccgcggcg acgtcgcggc
     2821 ggcgctgctc gagcagcagc cgcaccagcg cctgcacgcc ggacaggaag attcggccat
     2881 cgagcacgcg gtagcggtct tcgagtgcgg tcgcgcgcag cgctacatca gccggggcag
     2941 ccatagcgcg atctgcggaa agaggacgat cagcagcacg agcccgatcg tgcacaggat
     3001 gtaaggcagc gcggcgcgcg caacggtgcc gagcgtggtg cccctgggtg ccacgccgag
     3061 catcacgaac agcagcagcc cgaacggcgg agtggtgaag ccgatttcgt acgagagcag
     3121 c
//
LOCUS       DBNHBY010000736         3738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_393600, whole genome shotgun sequence.
ACCESSION   DBNHBY010000736 DBNHBY010000000
VERSION     DBNHBY010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_393600"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1384)
                     /gene="icmH"
                     /locus_tag="AC16HB_17325"
     CDS             complement(125..1384)
                     /gene="icmH"
                     /locus_tag="AC16HB_17325"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HIR5608606.1"
                     /translation="MNAADRVRAPGPEVELLKPDAPAAASKAGVELQRLVAGINPLLG
                     AASVLLALVAKLRATTMHADPSGLRRQLLARIEEFEASARAVGVPRLKIVTARYLLCT
                     FIDEAIAATPWGGSWNEHPLLQEFHDERWGGDKAFRLLERMGEDVAANADVLELFYVC
                     LALGFEGRYRGQPDGRAQLDAIAARLVQVVRPEIGRQATRELSLRWTGVRIPDRTRAV
                     LPLWVVFAVGAVMVIAAIVALNARLDAQSGPLFRQIVALPTALRSDLLDRAAAAARPR
                     LAAPLRAEIAAGALEVRDEVLRSVVVIPADALFAAGSAEIAAGQLELLARVAGALTGQ
                     PGQIAVIGHTDDQYRSSVQFPSNWELSRARASAVLQVLTQRGVPVERSHAEGRADAEP
                     RERSDTAAARARNRRIEIVLTLPRPES"
     gene            complement(1440..2846)
                     /locus_tag="AC16HB_17330"
     CDS             complement(1440..2846)
                     /locus_tag="AC16HB_17330"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5608607.1"
                     /translation="MNTPQRLGKYPITGVLGKGAMGAVYRGYDPVIKRPVAIKTIRKE
                     LIEEDAKAESLANRFRKEAQAAGALNHPGIVAIYEYGEDDEYAYIAMECVEGNTLRDY
                     LARGATFDERDTVSIMSQLLDALAFAHGHSIWHRDIKPANIMIMANGRIKLADFGVAR
                     IESSDRTQASVIMGTPGYIAPEQYLGQTCDLRMDIFSAGVLFYVLLAGQAPFRGRPEA
                     IMHDVCYVDPDPPSEVDPLHRWPKYDSIAARALQKKPELRYQSAAEFRSAILAAYARP
                     VSDAISESTIIATRSKPPAADPGAASAPKSTQIAGTSLPPPTGWDASALAKIEVELAR
                     FVGPVARVIVRRAARDHTDKFALVAVVGKAIELSEDRERFFLATTGQRSVAPVPVAAG
                     GATNPTQPTASGPAMTPQDVERATQLLAVYIGPIARVVAKRAAGAGISRSEFYARVAQ
                     TLDSQAQRERFLREAGVA"
     gene            complement(2855..>3738)
                     /locus_tag="AC16HB_17335"
     CDS             complement(2855..>3738)
                     /locus_tag="AC16HB_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tll0287-like domain-containing protein"
                     /protein_id="HIR5608608.1"
                     /translation="ENAMKLLLKFNLVFLLIFAVGLAAIGYVSWTLLQRNARAEIAQA
                     ARLLMDTAIATRSYTSTQVKPLLDTQMKYTFLPQSVPAYSATEIFNDLRKKHSEYSYK
                     EAVLNPTNPRDRAVEWETDIITQFRNSKDVNEVIGERETPAGGSYYFARPFRITDQGC
                     LYCHSTVDAAPKTMVERYGPANGFGWQLNEAVGAQIISVPTRVPLARAAEAFKVFMGS
                     VTAVFIAIGIVLNLMLYFIVIRPVAKLSQFADRVSLGELEIAEFKRESSDEIGVLARS
                     IGRMRTSMVQAMKMLEA"
BASE COUNT          569 a         1299 c         1274 g          596 t
ORIGIN      
        1 ggggcgagcg gccgcagtcc gccgagcgtg agcagcggcc cgaggtacca gatcagtgcg
       61 ctcgccgcga tcagccccgc agtcacgagc actggccggc tgagcagcgc cttgaacatg
      121 ctgttcatga ctcggggcgc ggcagcgtga gcacgatctc gatacgccgg ttgcgcgcgc
      181 gcgcggccgc cgtgtcgctg cgctcgcgcg gctcggcatc ggcgcggccc tccgcatgtg
      241 agcgttcgac gggcacaccg cgctgtgtca gcacctgcag taccgcgctg gcgcgcgcgc
      301 gcgacagttc ccagttcgaa gggaactgca ccgagctgcg atactgatcg tcggtgtggc
      361 cgatcacggc gatctgcccg ggctggcccg tcagcgcacc ggccacgcgc gcgagcaatt
      421 cgagctgacc tgcagcgatc tcggcgctgc cggccgcgaa cagcgcgtcg gccggtatca
      481 cgacgacgga acgcagtacc tcgtcgcgca cctcgagcgc cccggcggcg atttcggcgc
      541 gcagcggcgc tgccagccgc ggccgcgcgg ccgcggcggc gcggtcgagc agatcgctgc
      601 gcagcgccgt cggcagcgcg acgatctgcc ggaacagcgg accggattgc gcatcgagcc
      661 ttgcgttgag cgcgacgatc gccgcgatca ccatcaccgc cccgacggcg aacacgaccc
      721 acagcggcag caccgcgcgc gtccgatccg ggatccgcac cccggtccag cgcagcgaca
      781 gttcgcgtgt cgcctgtcgg ccgatctccg ggcgcacgac ctgcaccagg cgcgcggcaa
      841 tcgcgtcgag ctgcgcgcgg ccgtcgggct ggccacgata gcggccctcg aaaccgagcg
      901 cgaggcagac gtagaagagt tcgagcacgt cggcgttggc ggccacgtcc tcgcccatcc
      961 gctccagcag cctgaacgcc ttgtctccgc cccagcgctc gtcgtggaac tcctgcagca
     1021 gcgggtgctc gttccatgat ccgccccacg gcgtcgccgc gatcgcctcg tcgatgaacg
     1081 tgcacagcag gtagcgtgcc gtgacgatct tcaggcgcgg cacgccgacg gcgcgcgcgc
     1141 tggcctcgaa ctcctcgatg cgggcgagta gctggcgtcg cagcccggac gggtcggcgt
     1201 gcatcgtcgt cgcgcgcagc tttgcgacca gcgccagcag cacgctggca gcgccgagca
     1261 gcggattgat ccccgccacc agccgctgca gctcgacgcc ggccttcgac gccgcggccg
     1321 gagcgtccgg cttcagcagc tcgacctcgg gacccggggc gcggacgcga tcggcggcgt
     1381 tcattcagcg cttcgatgcc atgaagtccg gtcggggcgc gcgccgcccg gccgaccgct
     1441 caggcgacgc ccgcctcgcg caggaaccgc tcgcgctgcg cctggctgtc gagcgtctgg
     1501 gcgacccggg cgtagaactc actgcggctg atgcccgcgc cggctgcccg cttggccacc
     1561 acgcgggcga tcggcccgat gtagacggcg agtagttgcg tggcgcgctc gacgtcctgc
     1621 ggcgtcatcg ccgggccgga cgccgttggc tgcgtcggat tggtcgcgcc gcccgccgca
     1681 acgggcaccg gcgccaccga gcgctgaccc gtcgtggcga ggaagaagcg ctcgcgatcc
     1741 tcgctcagct cgatcgcctt gccgaccacg gccaccagcg cgaacttgtc cgtgtggtcg
     1801 cgcgccgcgc gccgcacgat caccctggcc acgggcccga cgaagcgcgc cagttcgacc
     1861 tcgatcttgg caagagccga cgcatcccaa cccgtgggcg gcggcagact ggtgccggcg
     1921 atctgcgtcg atttcggcgc cgatgccgcg ccgggatccg cggcgggcgg tttggagcgg
     1981 gtcgcgatga tggtcgactc ggagatcgcg tcgctcacgg gacgtgcata ggccgccagg
     2041 atcgccgagc ggaattcggc cgcgctctgg taacggagct cgggcttctt ctgcagcgcg
     2101 cgcgcggcaa tcgagtcgta cttgggccag cgatgcagcg gatcgacctc cgacggtgga
     2161 tcgggatcca cgtagcagac gtcgtgcatg atcgcctcgg gccggccgcg gaacggcgcc
     2221 tgcccggcga gcaggacgta gaacagcacg ccggcggaga agatgtccat gcgcaggtcg
     2281 cacgtctggc ccaggtactg ttccggcgcg atgtagcccg gcgtgcccat gatcacgctc
     2341 gcctgcgtgc ggtccgagga ctcgatgcgc gccacgccga agtcggcgag cttgatccgc
     2401 ccgttggcca tgatcatgat gttggccggc ttgatgtcgc ggtgccagat cgaatggccg
     2461 tgcgcgaagg caagcgcatc gagcagctgc gacatgatgc tgaccgtgtc gcgctcgtcg
     2521 aacgttgcgc cccgcgcaag atagtcgcgc agcgtgttgc cttcgacgca ctccatcgcg
     2581 atgtacgcgt actcgtcgtc ctcgccgtac tcgtagatcg cgacgatgcc gggatgattg
     2641 agcgcgccgg cggcttgcgc ctccttgcga aagcggttgg caagcgactc ggccttggcg
     2701 tcctcctcga tcagttcctt gcggatcgtc ttgatcgcca ccggccgctt gatcaccggg
     2761 tcgtagccgc ggtagacggc tcccatggca cccttgccga gcacgccggt gatcggatac
     2821 ttgccgagcc gctgcggggt gttcatgcgg cgcgtcaggc ctcgagcatc ttcatcgcct
     2881 gcaccatgct cgtccgcatg cgcccgatcg aacgcgccag cacgccgatc tcgtcgctcg
     2941 attcgcgctt gaactcggcg atctcgagtt cacccaggct gacgcggtcg gcgaactgcg
     3001 acagcttggc gaccggccgg atcacgatga agtagagcat caggttgagc acgatgccga
     3061 tggcaatgaa cacggcggtc accgacccca tgaagacctt gaaagcctcg gccgcgcgcg
     3121 ccagcggcac gcgcgtcggc acggaaatga tctgcgcacc gacggcctcg ttcaattgcc
     3181 agccgaaacc gttcgccgga ccatagcgct cgaccatcgt cttgggcgcc gcgtcgaccg
     3241 tgctgtggca gtacaggcag ccctggtcgg tgatgcggaa cgggcgcgcg aagtagtacg
     3301 agccgcccgc gggcgtttcg cgctcgccga tcacctcgtt gacgtccttg ctgttgcgga
     3361 actgcgtgat gatgtcggtt tcccactcga ccgcacggtc gcgcggattg gttggattca
     3421 gcaccgcctc cttgtagctg tactcgctgt gcttcttgcg cagatcgttg aagatctcgg
     3481 tcgccgaata ggctggcacc gactgcggca ggaacgtgta cttcatctgc gtgtcgagca
     3541 gcggcttgac ctgtgtcgac gtgtaggagc gcgtcgcaat cgcagtgtcc atcagtaggc
     3601 gcgcggcctg cgcgatttcg gcgcgcgcgt ttctctgcag cagcgtccag gacacgtaac
     3661 cgatcgccgc cagtccgacg gcaaaaatga gaagaaagac gaggttgaac ttcagcagaa
     3721 gtttcatcgc attctccg
//
LOCUS       DBNHBY010000737         3113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1003153, whole genome shotgun sequence.
ACCESSION   DBNHBY010000737 DBNHBY010000000
VERSION     DBNHBY010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1003153"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="AC16HB_17340"
     CDS             <1..1148
                     /locus_tag="AC16HB_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5608609.1"
                     /translation="LVEKSLVAMEADGARYRMLETVREYAREKLDLADDRDSAHDRHL
                     QHFVALAERMRPGLSGPEQGECLRTLDLELENILAATTWSGRTGRDELQWRLVEALRL
                     YWINRGLLSLGLRLNFSLLMRSDPPLDAAQRCRTLFGAGQISYLMGNYPHARAYLDEC
                     LRTARVAGDTRVAAGVLQPLGMVCLAERDWSAAQLHLENALALARESGDRREIAAALN
                     ALAMLHRMQERLDLAEPLYTEALELARGVGHREYVANILLNLAMVAIGRDATDRARAM
                     LREAAPIALETDSKPVVQGMLDVCAGLASAHSLWIDAARFFGMAEALRTATGLSRDPA
                     DEAFLLPRVDRARNALGAQSFAEHMTAGGAPATPDALAQAIAWLAVR"
     gene            complement(1149..1616)
                     /locus_tag="AC16HB_17345"
     CDS             complement(1149..1616)
                     /locus_tag="AC16HB_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608610.1"
                     /translation="MRLWRFLFAACPALAAGCAVPGLAPPLQERSHPQVEIRDGRIAL
                     SQEVLYFRRDERDVTVTWRLPEGSRLRFPANGIVIEGALVDKVIRSPQQRVEAVALDA
                     NQTEVVDCRVARDGLAFSCLNRNTRPGVYKYTIRIRDGDRVLERDPSLVNGNW"
     gene            complement(1716..>3113)
                     /gene="pbfA"
                     /locus_tag="AC16HB_17350"
     CDS             complement(1716..>3113)
                     /gene="pbfA"
                     /locus_tag="AC16HB_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0034386 - 4-aminobutyrate:2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-1-hydroxy-2-aminoethylphosphonate
                     ammonia-lyase"
                     /protein_id="HIR5608611.1"
                     /translation="REPGQSEGDVNLSTRRAQWQAAHLDAAARELLDRDGRAFLHQSV
                     STPCLNAIRKAEGIWIEDLAGRRYMDFHGNNVHHVGYAHPRVLAAIGDQLQKLSFAPR
                     RYACEPAVELAEKLAEVAPGNLSKVLFTTGGSDAVEVAIKLARAATGRFKTLSFWDAF
                     HGAGLGASGVGGESLFRSGPIGPLLAGTEHVAPFGCYRCPYGHAVDARGQPDIERCQL
                     ACASMVRYVLEREGDVAAVIAEPARAVPYLPPPGYWQAVRDACHAHGTLLVFDEIPTG
                     LGKTGRWFSCQHDAVEPDILVLGKSLGGGVLPIAAVIARPELDVAGAYAFGHYTHEKN
                     PVTCRAALETLRVIEDENLVDQAARVGAAALERIEGWRDRYALVGDVRGRGLLIGIEL
                     VADRADKTPDADAAEAVLYASLSNGLSFKTTMGNVLTLTPPLITTEAQMMRALDIVEA
                     AIASVQAARRRAGDA"
BASE COUNT          462 a         1118 c         1076 g          457 t
ORIGIN      
        1 gcctggtgga gaaatcgctg gtcgcgatgg aagcggacgg cgcacgctac cggatgctgg
       61 agaccgtacg cgagtacgca cgcgaaaagc tcgacctcgc ggacgatcgc gactcggcgc
      121 atgaccgtca cctgcaacac ttcgtcgcgc tcgccgagcg catgcggccg gggctgagcg
      181 gacccgagca gggcgaatgc ctgcgcacac tcgacctcga gcttgagaac atcctggcgg
      241 cgaccacgtg gagcgggcgc accgggcgcg acgagctgca gtggcggctg gtcgaggcct
      301 tgcggctcta ctggatcaac cgcggcctgc tgtcgctcgg cctgcggctc aatttcagcc
      361 tgctgatgcg cagcgatccg ccgctggacg ccgcgcaacg ctgccgcacg ctgttcggcg
      421 ccggacagat cagctacctg atgggcaact acccgcatgc gcgggcatat ctcgacgaat
      481 gcctgcggac cgcgcgcgtc gccggcgaca cgcgcgtggc tgccggcgtg ctgcagccgc
      541 tcggcatggt ctgcctcgcc gagcgcgact ggagcgcggc gcaactgcac ctggaaaacg
      601 cgctggcgct ggcacgcgaa tccggtgacc ggcgcgagat cgcggcagcg ctcaacgcgc
      661 tggcgatgct gcaccggatg caggagcggc tcgacctcgc cgagccgctc tacaccgagg
      721 cgctcgaact ggcgcgcggc gtcggtcacc gcgaatacgt ggcgaacatc ctgctgaatc
      781 tcgcgatggt ggcgatcggc cgcgatgcga ccgatcgggc gcgcgccatg ctgcgtgaag
      841 cggcgccgat cgcgctggaa accgattcga agccggtcgt gcagggcatg ctcgacgtgt
      901 gcgccggcct cgcttcggcg cattcgctat ggatcgacgc ggcgcgcttc ttcggcatgg
      961 cggaagcgct gcgcacggcc acggggctgt cgcgcgatcc cgccgacgag gcgttcctgc
     1021 tgccgcgcgt cgaccgcgcg cgaaacgcgc tcggcgcgca gtcgtttgcc gaacacatga
     1081 cggccggcgg cgcgccggcg acgccggacg cgctggcgca ggcgatcgcc tggctcgcgg
     1141 tccgttgact accagttccc gttcaccagc gacggatcgc gctcgagcac gcggtcgccg
     1201 tcgcggatcc ggatcgtgta cttgtagaca ccgggcctgg tgttccggtt caggcaactg
     1261 aatgccagac cgtcgcgcgc cacgcggcag tcgacgacct cggtctggtt cgcatcgagt
     1321 gcgaccgcct cgacgcgctg ctgcgggctg cggatcacct tgtcgacgag cgcgccctcg
     1381 atcacgatgc cgttggccgg gaagcgcagc cgcgagcctt ccggcaggcg ccaagtgacc
     1441 gtcacgtcgc gctcgtcgcg ccggaaataa agcacctcct ggctcaacgc gatccggccg
     1501 tcgcggatct cgacctgcgg atggctgcgt tcctgcagcg gtggcgccag gcccggaacc
     1561 gcgcagcccg cggcgagcgc cgggcaggcg gcgaacagaa agcgccagag tctcatcccc
     1621 ttgtcctcca tcgaatcggc ttgccacgcg tcgcctgatg atcgtacgtg agcttcgcgg
     1681 ccgcgacgcg ccctcgtccg gccgcgctcc ccaattcacg cgtcccctgc gcgtcggcgc
     1741 gccgcctgca ccgatgcgat cgccgcctcg acgatatcga gcgcccgcat catctgcgct
     1801 tcggtcgtga tcagcggcgg cgtcagcgtc agcacgttgc ccatcgtcgt cttgaacgac
     1861 aggccgttcg acagcgacgc gtacagcacc gcctcggcgg catcggcgtc cggtgtcttg
     1921 tccgcgcggt cggcaaccag ctcgatgccg atcagcaggc cgcgcccgcg cacgtcgccg
     1981 acgagggcat agcggtcgcg ccagccctcg atccgctcga gcgcggccgc accgactcgc
     2041 gccgcctggt cgacgaggtt ctcgtcctcg atcacgcgca aggtttcgag tgccgcgcgg
     2101 caagtgaccg ggttcttctc gtgcgtgtag tggccgaatg cgtacgcgcc ggccacatcg
     2161 agctcgggcc gcgcgatcac cgccgcgatc ggcagcacgc caccgccgag cgacttgccg
     2221 agcacgagga tgtcgggctc gaccgcgtcg tgctggcacg agaaccaacg cccggtcttg
     2281 cccagccccg tcgggatttc gtcgaatacg agcagcgtgc cgtgcgcatg gcacgcgtcg
     2341 cgcaccgcct gccagtaccc gggcggcggc aggtacggca ccgcgcgcgc gggctcggcg
     2401 atcaccgccg cgacatcgcc ctcgcgttcg aggacgtagc gcaccatcga cgcgcacgcg
     2461 agctggcagc gctcgatatc cggttggccg cgcgcgtcga ccgcgtgccc atagggacag
     2521 cgatagcagc cgaacggcgc gacgtgctcg gtgcccgcca gcagcgggcc gatcggcccc
     2581 gagcggaaca gcgactcgcc gccgacgccg gaggcgccga gtcccgcgcc gtggaacgcg
     2641 tcccagaacg acagcgtctt gaagcgcccg gtcgcggcgc gcgcgagctt gatcgcgact
     2701 tcgaccgcgt ccgagccgcc ggtggtgaac agcaccttcg acaggtttcc cggtgccacc
     2761 tcggccagct tctccgccag ttcgaccgcg ggctcgcacg cgtagcggcg cggcgcgaac
     2821 gagagcttct gcaactggtc gccgatcgcc gccaacacgc gcggatgcgc gtaaccgacg
     2881 tgatgcacgt tgttgccgtg gaagtccatg tagcggcggc cggccagatc ctcgatccag
     2941 atgccttcgg ccttgcggat cgcattcagg cacggcgtcg acaccgactg gtgcagaaag
     3001 gcgcggccgt cgcggtcgag cagttcgcgc gcggccgcat cgaggtgcgc cgcctgccat
     3061 tgcgcgcggc gggtcgacag gttgacgtcg ccttcggact ggcccggttc gcg
//
LOCUS       DBNHBY010000738         3094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1544461, whole genome shotgun sequence.
ACCESSION   DBNHBY010000738 DBNHBY010000000
VERSION     DBNHBY010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1544461"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3094)
                     /locus_tag="AC16HB_17355"
     CDS             complement(<1..>3094)
                     /locus_tag="AC16HB_17355"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5608612.1"
                     /translation="VCDDDLWRVDADGGIARRLTAGVGEPSTPALSPQGQWIAYVGRD
                     ELHPEVYVMPAEGGPARRMTWLGPDVMVRGFTPDGRVLFVTTHGQPFFRNYRAYGLAL
                     DGGLPELLPLGQVNHLAFGPNGAKVIGRNTADPARWKRYRGGTAGHLWIDADGSGEFR
                     RMTELKGNITSPMWVGERVYFLSDAEGYGNLYSCLPDGSDLKRHSDHDDYYARHASTD
                     GARIVYQCGAALWLFDPRTDRARRLDIRVPAHRAQAARRFVPAAEHLMQFNVHPAGHG
                     LAVETRGKMFSFALWEGAVLQHGQVDGARYRHAQWLADGTTLVAVGDASGEERLEIFD
                     GTTSRALPWDIGRVLALAVAPKGRKLALANHRNEVLLADLDNDTLALVDRSDAGRTEE
                     LAWSPDGAWLAYPFWTGLRHCAIKLHEVARARSVLVTTPEFRDWSPAFDPEGKYLCFL
                     SVRTFDPVYDNVQFELSFPRAARPYLIALAADARPPFEPPPKGMKRTWDDGPRDDEKK
                     HADVTVKVDLDGIVQRVAAFPVPEGRYGRIAGLRHKAAWTLLPIPGAHGRGGHKETPG
                     RLEVFDFDTLRVDTRLERADDFALSADGTTMIVRDGQKLRAIDPLHKAHHDEAQSRSD
                     EPSRASGWIDLARVRVSVDPRAEWRQMLREVWRLQRDQFWVADMSGIDWDAVYRRYVP
                     LLELVATRSELSDLIWEMQGELGTSHAYEMGGDHRKPPPVALGHLGADLKLATDGTSY
                     EIVHVVAGEPWDASADSPLNAIGVQARAGERIVAVNGQPVSRERPPQAMLVHQSGMKA
                     ELTLGSDAGARTVLVKLLADEVPVRYREWVERNRAWVHERSNGRVGYLHLPDMMSAGF
                     AEFHRYFGAECDRDALIVDVRYNRGGHVSQLLLEKVARQRVGYDLARWGKPTPYPLES
                     PAGPVVALTNEHAGSDGDIFSHCFKLMGIGKLVGTRTWGGVIGIWPRHKLADGTETTQ
                     PEFSFWFKDVGWGVENYGTDPQIEVDNAPQDAAAGRDRQLERTLEVALQAIAHTKPAL
                     P"
BASE COUNT          455 a         1102 c         1047 g          490 t
ORIGIN      
        1 gcggcagcgc cggcttcgtg tgcgcaatcg cctgcagcgc gacctcgagc gtcctttcca
       61 gctgtcgatc gcgtccggcg gccgcgtcct gcggtgcgtt atcgacctcg atctggggat
      121 cggtgccgta gttctccacg ccccagccga cgtccttgaa ccagaacgag aactccggct
      181 gcgtggtctc cgtgccgtcg gcgagcttgt gtcggggcca gatgccgatc acgccacccc
      241 aggtgcgcgt gccgaccagc ttgccgatcc ccatcagctt gaagcagtgc gaaaagatat
      301 ctccatcgga gccggcgtgc tcgttggtca gcgccaccac cggaccagcc ggcgattcga
      361 gcggatacgg cgtgggcttg ccccagcgcg cgaggtcgta gccgacccgc tgccgcgcga
      421 ccttctccag caacagttgc gacacgtgac cgccgcggtt gtagcgcacg tcgacgatca
      481 gcgcgtcgcg gtcgcactcg gcaccgaagt aacgatggaa ctccgcgaaa ccggcgctca
      541 tcatgtcggg caggtgcagg tagccgacgc ggccattgga ccgctcgtgc acccaggcgc
      601 gattgcgttc gacccattcg cggtaacgca cgggcacttc gtcggcgagc agtttcacca
      661 gcacggtgcg ggcgccggcg tcggacccga gtgtcagttc ggccttcatg cccgattggt
      721 gcaccagcat cgcctgcggc ggacgctcgc gcgacaccgg ctggccgttg acggcgacga
      781 tgcgctcacc cgcgcgcgcc tgcacgccga tcgcgttcag gggcgaatcg gcgctcgcat
      841 cccagggctc gcccgcgacg acgtgcacga tttcatagct cgtgccgtcg gtggcgagct
      901 tcaggtccgc gcccagatga ccgagcgcca ccggcggcgg tttgcggtgg tcgccgccca
      961 tctcgtacgc gtgcgacgtg ccgagttcgc cctgcatttc ccagatcagg tccgacagtt
     1021 cgctgcgcgt ggccacgagt tcgagcagcg gcacgtagcg gcgatacacg gcatcccagt
     1081 cgattccgga catgtcggcg acccagaact ggtcgcgctg cagccgccac acttcgcgca
     1141 gcatctgccg ccattcggcg cgcggatcga ccgacacgcg cacgcgcgcc agatcgatcc
     1201 agccgctggc ccgcgacggt tcatcgctcc gcgattgcgc ctcgtcgtgg tgcgccttgt
     1261 gcagcggatc gatcgcgcgc agcttctggc cgtcgcgcac gatcatcgtg gtgccgtccg
     1321 ccgacagcgc gaagtcgtcc gcgcgctcca accgcgtgtc cacgcgcaag gtgtcgaaat
     1381 cgaatacctc gaggcggccc ggcgtttcct tgtgtccgcc gcggccgtgc gcgccgggaa
     1441 tcggcagcag cgtccacgcc gccttgtgcc gcagtcctgc gatgcggccg tagcggccct
     1501 cgggaaccgg gaacgcggcc acgcgctgca cgatgccgtc gagatcgacc ttcaccgtga
     1561 catcggcgtg cttcttctcg tcatcgcgcg gaccgtcgtc ccaggtgcgc ttcatgccct
     1621 tcggtggcgg ttcgaacggc ggacgggcgt ctgccgcgag cgcgatcagg tacggccgcg
     1681 cggcgcgcgg gaaactcagc tcgaactgca cgttgtcgta caccgggtcg aacgtgcgca
     1741 ccgacaggaa gcacaggtac ttgccctccg gatcgaacgc cggactccaa tcgcggaact
     1801 ccggcgtcgt tacgagcacg ctccgcgcgc gcgcgacctc gtgcagcttg atcgcgcagt
     1861 ggcgcaggcc ggtccagaac ggatacgcga gccaagcgcc gtccggcgac catgcgagtt
     1921 cctcggtgcg gccggcgtcg ctgcggtcga ccagagccag cgtgtcgttg tcgagatcgg
     1981 cgagcaggac ctcgttgcgg tgattcgcca gcgccagctt gcggcccttc ggcgccacgg
     2041 cgagcgccag cacgcggccg atatcccacg gcagcgcacg gctcgtggtg ccgtcgaaga
     2101 tttcgagccg ctcctcgccc gacgcgtccc cgaccgcgac caatgtcgtg ccgtcggcca
     2161 gccactgcgc gtgccggtac cgcgcgccgt cgacctggcc gtgctggagc accgcgccct
     2221 cccaaagtgc gaacgaaaac atcttgccgc gcgtttcgac ggcaaggccg tggccggccg
     2281 gatgcacatt gaactgcatc aggtgctcgg ccgcgggcac gaagcgccgc gccgcctgcg
     2341 cgcgatgcgc gggcacgcgg atgtcgagtc ggcgcgcgcg gtccgtgcgg ggatcgaaca
     2401 gccatagcgc cgcgccgcac tggtacacga tgcgcgcgcc gtcggtcgac gcatggcgtg
     2461 cgtagtagtc gtcgtggtcc gaatggcgct tcagatcgct gccgtcgggc aggcacgagt
     2521 acagattgcc gtacccctcc gcatccgaga ggaagtacac gcgctcgccg acccacatcg
     2581 ggctcgtgat gttgcctttc agttccgtca tgcggcgaaa ttcgccgctg ccgtccgcgt
     2641 cgatccacag gtgcccggcg gtgccgccgc gatagcgctt ccaacgcgcc gggtcggcgg
     2701 tgttgcggcc gatcaccttg gcgccgttgg gtccgaacgc gaggtgattg acctgcccga
     2761 gcggcagcag ttcgggcagt ccgccgtcga gcgcgaggcc gtacgcgcgg tagttgcgga
     2821 agaacggctg gccgtgcgtg gtgacgaaga gcacgcggcc gtcgggcgtg aagccgcgca
     2881 ccatcacgtc ggggccgagc catgtcatcc gccgcgcggg gccgccttcg gccggcatca
     2941 cgtacacctc ggggtgcagc tcgtcgcgcc cgacgtaagc gatccactgc ccttgcggcg
     3001 agagcgccgg cgtcgacggc tcgccgacgc cggcggtcag ccggcgcgcg atgccgccat
     3061 ccgcatcgac gcgccacagg tcgtcgtcgc agac
//
LOCUS       DBNHBY010000739         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_597834, whole genome shotgun sequence.
ACCESSION   DBNHBY010000739 DBNHBY010000000
VERSION     DBNHBY010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_597834"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..272
                     /locus_tag="AC16HB_17360"
     CDS             21..272
                     /locus_tag="AC16HB_17360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5608613.1"
                     /translation="MAKSTPKPVAELSFEQALDELDALVRRMEAGELSLDDSIAAYRR
                     GAELAKYCQSKLAAAEQEIKKLDGELLVPLNPDELRGNP"
     gene            273..1211
                     /locus_tag="AC16HB_17365"
     CDS             273..1211
                     /locus_tag="AC16HB_17365"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5608614.1"
                     /translation="MHGAVKPVAAVATAAEQDFDAWSAERLARFERAAEAQLPLPTVA
                     PQRLHEAMRYAVLGGGKRVRPLLVYAAGEVTGASLVALDGAALATEYVHVYSLIHDDL
                     PCMDNDVLRRGRPTVHVAFDEATAMLAGDGLQAEAFKVLADTDLPDRARAALMRELAH
                     AASTAGMCGGQALDLAAVGRTLTLEELERMHRMKTGALLLAAVRMGAHSGQVDAATAA
                     ALDGYGRAIGLAFQVVDDVLDVEQSSHALGKTAGKDAQQGKPTYVSALGLAGAKEWAE
                     TLRRRAHAALDALAGPAAARVLRLRQLADFIVVRSC"
     gene            1230..3125
                     /gene="dxs"
                     /locus_tag="AC16HB_17370"
     CDS             1230..3125
                     /gene="dxs"
                     /locus_tag="AC16HB_17370"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR5608615.1"
                     /translation="MYDLLATIRSPADLRRLDGERLKRLAGELRAFVLDSVSRTGGHL
                     SSNLGTVELTIALHHVFDTPRDRIVWDVGHQTYAHKILTGRREAMKSLRQFGGISGFP
                     SRRESEYDAFGTAHSSTSISAALGMAVAARASGNKRHNIAVIGDGALSGGMAFEALNN
                     AGVIDGIRLLVILNDNDMSISPAVGALNRYLARLMSGKFYAAARQAGKQVLKNFPPVL
                     ELARRFEEHAKGMVVPATLFEEFGFNYIGPIDGHDLDSLIPTLANIRQLTGPQFLHVV
                     TRKGQGYKLAEADPVLYHGPGKFDPKQGIQPAAPSKPTFTQVFGDWLCDTAAADLRLV
                     GITPAMREGSGMVRFEQEHPDRYFDVGIAEQHAVTFAAGLAAEGMKPVLAIYSTFLQR
                     GYDQLIHDVAIQNLPVLFALDRGGLVGADGATHHGAFDLSYLRCIPNMAVIAPSDENE
                     CRRLLTTAFQHHGPAAVRYPRGAGIGAPIEHELQGLPFGKGEIRRESAARPGSRIAVL
                     AFGPVLHPALAAAAKFDATVVDMRFVKPLDLELIERIARTHDALVTVEENVVKGGAGA
                     GCAEALADLGIVKPILHLGLPDQFVDHGDPARLMASVGLDAVGIEQSIRIRFGDLQTQ
                     PRLVKSA"
     gene            3140..3880
                     /locus_tag="AC16HB_17375"
     CDS             3140..3880
                     /locus_tag="AC16HB_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR5608616.1"
                     /translation="MKPVAVFQHSPEAGPGYFGEWLAAQRISMRLIRIDRGVAVPPRA
                     DAFGGLCFMGGPMSVNDPLPWIDQELRLIGDADARGVPVIGHCLGGQLLAKALGGAVT
                     RSPVKEIGWGRLRVTAAAVAREWLGAEATDTPEFFQWHGDTFSLPPGAMNFLASDLCA
                     HQAFVIERDGCAHLGMQFHCEMTPALIEDWATDAEGVAEIAEERRVNGGPGVSDVDAM
                     MRDMAVRTQRMNALAARLYARWAQGIGR"
     gene            3999..>4177
                     /locus_tag="AC16HB_17380"
     CDS             3999..>4177
                     /locus_tag="AC16HB_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5608617.1"
                     /translation="MAQRPGDDAPDFTAETTEGTIRFHEWIGDGWAILYSHPKDFTPV
                     GTTELGYMAALKKGF"
BASE COUNT          664 a         1379 c         1480 g          654 t
ORIGIN      
        1 acggccggta caatcgcgcg atggccaagt cgacacccaa gcccgtcgcg gagttgagtt
       61 tcgagcaggc gctggacgaa ctcgacgcgc tcgtgcggcg catggaagcg ggcgagctgt
      121 cgctcgatga ctcgatcgcc gcctaccggc gcggcgctga actggcaaag tactgccagt
      181 cgaagctcgc cgcggccgaa caggaaatca agaaactcga cggcgagctg ctggtgccgc
      241 tgaatcccga cgagctgcgc ggcaatccgt agatgcacgg cgccgtcaag ccagtggccg
      301 cggtcgcgac ggccgcggag caggacttcg acgcctggag tgcggagcgc ctggcgcgct
      361 tcgagcgcgc cgccgaagcg caattgccgc tgcccacggt cgccccgcaa cggctgcacg
      421 aggcgatgcg ctacgcggtg cttggcggtg gcaagcgcgt gcgcccattg ctcgtctacg
      481 ccgccggcga agtgaccggc gcgtcactgg tggcgctcga tggcgccgcg ctcgcgaccg
      541 agtacgtcca cgtgtattcg ctgatccacg atgacctgcc gtgtatggat aacgacgtgc
      601 tgcggcgggg tagaccgacc gtgcacgtcg ccttcgacga agcgacagcg atgctggccg
      661 gcgacggcct gcaggccgaa gcgttcaagg tgctggccga cacggatctg cccgatcgcg
      721 cgcgcgccgc gctgatgcgc gaactcgcgc acgcggcgtc gacggcgggc atgtgcggcg
      781 ggcaggcgct cgacctggcc gcggtcgggc gcacgctgac gctcgaggaa ctggaacgga
      841 tgcatcggat gaagaccggc gcgctgctgc tcgcggcggt gcgcatgggc gcacactccg
      901 ggcaggtcga tgcggcgacc gccgcggcgc tcgatggcta cggccgggcg atcgggctgg
      961 cgttccaggt cgtcgacgac gtgctcgacg tggagcagag ttcgcacgcg ctgggcaaga
     1021 ccgccggcaa ggacgcacag cagggcaagc cgacctacgt atccgcgctc ggcctcgcag
     1081 gtgcgaagga gtgggccgag acgctgcgcc gcagggccca cgccgcgctc gacgcgctgg
     1141 ccggcccggc cgcggcgcgc gtgctgcgtc tgcgacaatt ggccgacttc atcgtcgtgc
     1201 gctcctgctg agcggcgacc tcggttctca tgtacgacct gctcgcaacg atccgatccc
     1261 ccgccgacct gcgcagactc gacggcgagc gcttgaaacg cctggccggc gagttgcgcg
     1321 cattcgtgct cgactcggtg tccaggaccg gcgggcatct gtcgtcgaac ctcggcacgg
     1381 tcgagctgac catcgcgctg catcacgtat tcgatacgcc gcgcgaccgc atcgtgtggg
     1441 acgtcggcca ccagacctac gcccacaaga tcctgaccgg ccgccgcgag gcgatgaagt
     1501 cgctgcgcca gtttggcggc atctcgggat ttccgagccg gcgcgagtcg gagtacgacg
     1561 cgttcggcac cgcgcattcg tcgacttcga tctccgcggc gctcggcatg gcggtcgcgg
     1621 cgcgtgcgag cggcaacaag cgccacaaca tcgcggtgat cggcgacggc gcgctgtctg
     1681 gcggcatggc gttcgaggcg ctgaacaacg ccggcgtcat cgatggcatc cgcctgctgg
     1741 tgatcctgaa cgacaacgac atgtcgatct cgcccgccgt tggcgccctg aaccgctatc
     1801 tggcacggct gatgagcggc aagttctacg cggcggcgcg ccaggccggc aagcaggtgc
     1861 tgaagaactt cccgcccgtg ctggaactgg cgcggcgctt cgaggagcac gcgaagggca
     1921 tggtcgtgcc cgcgacgctg ttcgaggagt tcggcttcaa ctacatcggt ccgatcgacg
     1981 gccacgacct cgactcgctg atcccgacgc tcgcgaacat caggcagctc accggcccgc
     2041 agttcctgca cgtcgtcacg cgcaaggggc agggctacaa gctcgccgag gcggacccgg
     2101 tcctgtatca cggtcccggc aagttcgatc ccaagcaggg catccagccg gccgcgccga
     2161 gcaagccgac cttcacgcag gtgttcggcg actggctgtg cgacacggcg gccgcggacc
     2221 tgcggctcgt cggcatcaca ccggcgatgc gcgagggttc gggcatggtg cgattcgagc
     2281 aggaacatcc tgaccgctat ttcgatgtcg gcatcgccga gcagcatgcg gtgacgttcg
     2341 cggccggact cgcggccgag ggcatgaagc cggtgctggc aatctattcg accttcctgc
     2401 agcgcggcta cgaccaactg atccacgacg tcgcaatcca gaacctgccg gtgctgttcg
     2461 cgctcgaccg cggcgggctg gtcggcgccg acggcgccac gcatcacggc gcgttcgacc
     2521 tgtcgtacct gcgctgcatc ccgaacatgg ccgtgatcgc gccctcggac gagaacgagt
     2581 gcaggcggct gctgaccacg gcgttccagc accacggccc ggccgcggtg cgctatccgc
     2641 gcggcgcagg gatcggcgcg ccgatcgagc acgagctgca ggggttgccg ttcggcaagg
     2701 gcgagatcag gcgcgaatcg gccgcgcggc cgggcagccg gatcgccgtc ctcgcgttcg
     2761 gccccgtgct gcatcctgcg ctggcggccg cggcaaagtt cgatgcaacg gtcgtcgaca
     2821 tgcgcttcgt caagccgctg gatctcgaac tgatcgagcg catcgcccgc acgcacgacg
     2881 cgctggtcac tgtcgaggag aacgtcgtca agggcggcgc cggcgcgggc tgcgccgagg
     2941 cgctggcgga tctgggtatc gtcaagccga tactgcacct cggtctgccc gatcagttcg
     3001 tggaccacgg cgatccggcg cgactgatgg cgagcgtggg cctggacgca gtcggcatcg
     3061 agcagtcgat ccgcattcgc ttcggcgatc tgcagacgca gccgcgactg gtgaagtcgg
     3121 cctgagcgtc gattccgcga tgaagccggt cgcggtgttc cagcattcgc ccgaggccgg
     3181 accgggctac ttcggcgagt ggctcgccgc gcagcgaatt tcaatgcggt tgatccgtat
     3241 cgaccgcggc gtcgcggtgc ctccgcgcgc cgatgcattc ggcggcctgt gcttcatggg
     3301 cgggccgatg agcgtcaacg atccgctgcc gtggatcgat caggaactgc gcctgatcgg
     3361 cgacgccgat gcgcgcggcg ttccggtgat cggccattgc ctcggtggcc aactgctcgc
     3421 caaggcgctc gggggtgccg tcacccgcag tccggtgaag gagatcggct ggggacgatt
     3481 gcgcgtgacc gctgcggcgg tggcgcgcga gtggctcggc gccgaggcaa cggatacgcc
     3541 ggagttcttc cagtggcacg gcgacacgtt ttcgctgccg cccggcgcga tgaactttct
     3601 cgccagcgac ctgtgcgcgc accaggcatt cgtgatcgaa cgtgacggct gcgcgcatct
     3661 gggcatgcag tttcactgcg agatgacgcc ggcgctgatc gaggactggg cgaccgatgc
     3721 cgagggtgtt gcggaaattg ccgaagagcg ccgcgtcaac ggcggcccgg gggtcagcga
     3781 cgtcgacgcg atgatgcgcg atatggccgt gcgcacgcag cggatgaacg cactggccgc
     3841 gcgcctgtat gcacggtggg cgcaggggat tgggcgctag cacccgcgcg tggcgcgggc
     3901 atcgggtcaa gatgccaggg atgacttcgt tgcggtcgtg gacttctcgg ggctatgttt
     3961 gcagtccttt gtgtgtgtag ctcgcagtgg agaaacccat ggcgcagcga ccgggcgacg
     4021 acgctcccga cttcaccgcc gaaacgaccg aaggaacgat ccgctttcac gaatggatcg
     4081 gcgacggctg ggcgatcctg tactcgcatc cgaaggactt cacccccgtt ggcacgacgg
     4141 aactcggtta catggccgct ctgaagaagg ggttcga
//
LOCUS       DBNHBY010000740         4472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1611829, whole genome shotgun sequence.
ACCESSION   DBNHBY010000740 DBNHBY010000000
VERSION     DBNHBY010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1611829"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="AC16HB_17385"
     CDS             complement(<1..1635)
                     /locus_tag="AC16HB_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR5608618.1"
                     /translation="MNLDDLAKLATSASWRLPEADADPQETQEWLDALDAIIHDVGPQ
                     RATFILQKLVQHARRRRVQLPTVANTPYINTISLAQQAPFPGNLELEARLSALVRWNA
                     LAMVVRANRASAELGGHIASYASAADLFEVGFNHFFRAGQAGDLVYFQPHSAPGVYAR
                     AYLEGRLSEQQLSNYRRETGGNGLSSYPHPWLMPDFWQFPTGSMGIGPLFAIYQARFM
                     RYLAHRGVLDTQGRKVWAFVGDGEMDEPEALAGLSIAAREGLDNLVFVVNCNLQRLDG
                     PVRGNGSIIQELEGLFAGAGWNVIKLLWGSDWDPLFARDEHGLLLRAFHETVDGEFQQ
                     LSATSGRFNREHFFAKFPELQQLVAHLSDDDIDRLRRGGHDPVKIHAAYHAAANHRGQ
                     PTVILAKTKKGYGMGSAGQGRMTTHQQKKLDTQQLLEFRDRFALPLSDQQVDACEFVR
                     PIAASPEMKYLHARRAKLGGYLPLRPRRGDAVPLPSLEAYAKFALEANGKEMNTTMAL
                     VRMIGALLKDREVGPRIVPIIADEARTFGMADLFRQI"
     gene            1695..2747
                     /gene="pyrC"
                     /locus_tag="AC16HB_17390"
     CDS             1695..2747
                     /gene="pyrC"
                     /locus_tag="AC16HB_17390"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5608619.1"
                     /translation="MVERIDLTRPDDWHLHLRDGAQMADVLPYTARQFARAIVMPNLR
                     PPVTTTALAAAYRERILRAVPRGLAFEPLMTLYLTDNTPADEIARAKESGFVHGVKLY
                     PAGATTNSDSGVTDLQKCVRALEAMQRLDLPLLVHGEVTAGEVDVFDREARFIDDVLI
                     PLRRDFPGLRVVFEHITTREAVDYVTSAAGRIAATITAHHLLYSRNAIFMTAGGRTGI
                     RPHYYCLPVLKREMHRHALLAAAASGNPRFFLGTDSAPHPKGDKENACGCAGCFTAAS
                     ALEMYATAFEQAGTLGRLEAFASFHGPDFYGLPRNTGRVTLERRAWTLPAELEFGEQT
                     IVPLAAGEEMAWKLVP"
     gene            2729..3484
                     /locus_tag="AC16HB_17395"
     CDS             2729..3484
                     /locus_tag="AC16HB_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HIR5608620.1"
                     /translation="MEARAVIEWSAPWLAPHAALRPAFELPDWRGELTRLARERSVRG
                     SGGHPIEFVAANDAPPGVAYEAHIAATGRVPSRGNLHDRFNALMWLAFPRLKAALNAR
                     QAVEIARLGVGDTRGAVRDAATLIDESGLLLACDDTSVFELLSHRQWHALLVERRARW
                     GVDIVPFAFGHALLEKLAGPYKAITAAVVPVRGTTVESIGAAAAEFVSRPDLAPRWLE
                     HLPVLGIPGWCADNEDARFYDDERVFRPLRRAA"
     gene            3497..>4472
                     /gene="denD"
                     /locus_tag="AC16HB_17400"
     CDS             3497..>4472
                     /gene="denD"
                     /locus_tag="AC16HB_17400"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HIR5608621.1"
                     /translation="MKIVITGGAGFLGQLLVRALAARGSLTDAAGRERRIERIVAIDQ
                     SRSDQLFVDERVGYVVGDIAAAGFVEHVMESNTDSVFHLAAVVSGAAEADFDLGMRVN
                     LDGTRAILNACRKQTGKPKLVFASSVAVFGGELPAVVSDETQPTPQSSYGVQKLVCEL
                     LIGDCSRKGFIDGRSVRLPTIVVRPGKPNAAASSFASGIVREPLAGEISACPVDSSTA
                     MWVLSPRSAITAFVHAHELPAERWDARRALNLPGLTVTVAQMLEALRAVGGDAAVALV
                     KMQPDERIAAIVRTWPARFATTRADALGFARDADVGSIVRAYAREYAAG"
BASE COUNT          688 a         1503 c         1566 g          715 t
ORIGIN      
        1 gatctggcgg aacaggtcgg ccatgccgaa cgtgcgcgcc tcgtcggcga tgatcggcac
       61 gatgcgcgga ccgacttccc ggtctttcag cagcgcgccg atcatgcgca ccaacgccat
      121 cgtcgtgttc atctccttgc cgttggcttc gagcgcgaac ttcgcatatg cctcgagcga
      181 cggcagtggc acggcatcgc cgcggcgcgg acgaagcggc aggtagcccc ccagcttggc
      241 gcgccgcgcg tgcaggtact tcatctccgg actggcggcg atggggcgca cgaattcgca
      301 ggcatcgacc tgctggtccg acagggggag cgcaaagcga tcgcggaatt cgagcaattg
      361 ctgtgtgtcg agcttcttct gctggtgcgt cgtcatgcgc ccctggcccg cgctgcccat
      421 gccgtagccc ttcttcgtct tggccaggat cacggtcggc tgtccgcgat ggttcgccgc
      481 cgcgtggtag gcggcgtgga tcttcaccgg gtcgtggccg ccgcggcgca gacggtcgat
      541 gtcgtcgtcc gacaggtgcg caaccagctg ctgcagctcc ggaaacttgg cgaagaagtg
      601 ctcgcggttg aagcggccgc tggtggccga gagctgctgg aactcgccgt cgaccgtctc
      661 gtggaacgcg cgcagcagca ggccatgttc gtcgcgtgcg aacagcggat cccagtcgct
      721 gccccacaac agcttgatca cgttccagcc ggcgccggcg aacaggccct cgagttcctg
      781 gatgatcgag ccgttgccgc ggaccgggcc gtcgagccgc tgcaggttgc agttgacgac
      841 gaacaccagg ttgtcgagcc cctcgcgcgc ggcgatcgac agcccggcga gcgcctcggg
      901 ctcgtccatc tcgccgtccc cgacgaacgc ccacaccttg cgcccctgcg tgtcgagcac
      961 gccgcgatgc gcgaggtagc gcatgaagcg cgcctggtaa atcgcgaaca acgggccgat
     1021 gcccatcgag cccgtcggaa attgccagaa gtccggcatc agccaggggt gcggatacga
     1081 ggacaggccg ttgccgcccg tctcgcgacg gtagttcgac agctgctgtt ccgaaagtcg
     1141 tccctcgaga taggcgcgcg catacacgcc gggtgccgag tgcggctgga aatagacgag
     1201 gtcacccgcc tggccggcgc gaaaaaagtg attgaacccc acctcgaaca gatccgccgc
     1261 cgacgcgtag ctcgcgatgt gtccgccgag ctcggcgctg gcgcggttgg cacgcaccac
     1321 catcgccagc gcgttccagc gcaccagcgc tgacaagcgc gcttcgagct ccaggttgcc
     1381 cggaaacggt gcctgctgcg ccagcgaaat cgtgttgatg tagggcgtgt tggcgacggt
     1441 cggcaactgc acgcggcgcc ggcgcgcgtg ctgcaccagc ttctgcagga tgaacgtcgc
     1501 gcgctgcgga ccgacgtcgt ggatgatcgc gtcgagcgcg tcgagccatt cctgcgtctc
     1561 ctgcggatcg gcgtcggctt cgggcagacg ccacgacgcg ctcgtggcga gcttggcgag
     1621 atcgtcgagg ttcatggtgg cgtctccggt cgaacaaatt atcgccccgc tgggataatg
     1681 gcagcgaagg agacatcgtg gaacgcatcg atctgacccg tcccgacgat tggcacctgc
     1741 atctgcgcga tggcgcgcag atggccgatg tcctgccgta caccgcgcgc cagttcgcgc
     1801 gcgcgatcgt gatgccgaac ctgcggccgc cggtgaccac gactgccttg gccgccgcgt
     1861 atcgcgagcg catcctgcgc gcagttccgc gtgggctcgc gttcgagccg ctgatgacgc
     1921 tctacctgac cgacaacacg ccggccgacg agattgcgcg cgcgaaggag tccggcttcg
     1981 tgcacggcgt gaagctgtat ccggccggcg cgacgaccaa ctcggattcc ggcgtaaccg
     2041 acttgcagaa gtgtgtgcgc gcactggagg cgatgcagag actcgacctg ccactcttgg
     2101 tgcacggcga ggtcactgcg ggggaggtcg acgtgttcga ccgcgaggcg cgtttcatcg
     2161 acgacgtgct gatcccgttg cggcgcgact tcccgggctt gcgcgtcgtg ttcgagcaca
     2221 tcaccacgcg cgaggctgtc gattacgtga cgtccgccgc cggccgcatc gccgccacga
     2281 tcaccgcgca tcacctgctg tacagccgca atgcgatctt catgaccgcg ggcggcagga
     2341 ccggcatccg accgcactac tactgcctgc cggtgctcaa gcgcgagatg caccgccacg
     2401 cgctgctggc ggcggcggcc tctggcaatc cgcgcttctt cctcggcacc gacagcgcgc
     2461 cccatccgaa aggcgacaag gaaaacgcat gcggctgcgc cggctgcttc actgccgcct
     2521 cggcgctcga gatgtacgcg accgcgttcg aacaggccgg tacgcttggc agactcgagg
     2581 cgttcgcgag cttccatggc ccggatttct acggactgcc gcgcaatacc ggccgcgtga
     2641 cactcgagcg gcgcgcgtgg acgctgcccg cggaactcga attcggcgaa cagacgatcg
     2701 tcccgctcgc tgcgggcgaa gagatggcgt ggaagctcgt gccgtgatcg agtggtcggc
     2761 gccgtggctg gctccgcatg ccgccttgcg gccggcgttc gaactgcccg actggcgcgg
     2821 cgaactgacg cggctcgcgc gcgagcgcag cgtgcgtggg tcgggcggtc atccgatcga
     2881 attcgtcgcc gcgaacgatg cgccgccggg agtggcgtac gaagcgcaca tcgcagccac
     2941 cggccgtgtg ccgagccgcg gcaatctgca cgaccgcttc aacgcgctga tgtggctcgc
     3001 gtttccgcgg ctgaaagcgg cgctcaatgc gcgccaggcc gtcgagatcg cgcggctcgg
     3061 cgttggcgac acgcgcggcg cggtgcgcga tgccgcgacg ctgatcgacg aaagcgggct
     3121 gctgctcgcc tgcgacgaca cctcggtgtt cgagttgctg agtcacaggc aatggcacgc
     3181 gctgctcgtc gagcggcgcg cgcgctgggg cgtggacatc gtcccgttcg cgttcggcca
     3241 tgccctgctg gagaagctcg ccggcccgta caaggcaatc accgccgcag tggtgccggt
     3301 gcgagggacg acggtcgagt cgatcggcgc cgcagcggcc gagttcgtct cgcggccgga
     3361 tctcgcgccg cggtggctcg aacacctgcc ggtgctcggc attccgggct ggtgcgcgga
     3421 caacgaggac gcgcggtttt acgatgacga gcgcgtgttc cgcccgctgc ggcgcgctgc
     3481 ctaggagggg aacgcaatga agatcgtgat cacgggcggc gccggtttcc tcgggcaact
     3541 gctggtgcgc gcgcttgcgg cgcgcggcag cctcaccgat gccgcaggcc gcgagcggcg
     3601 catcgagcgg atcgtcgcga tcgaccagtc gcggagcgac cagctgttcg tcgacgaacg
     3661 cgtcggctac gtggtcggcg acatcgccgc agcaggtttc gtcgagcatg tgatggagag
     3721 caacacggac agcgtgttcc atctggcggc cgtcgtcagc ggcgcggcgg aggccgactt
     3781 cgaccttggc atgcgcgtga acctcgacgg cacgcgcgcg atcctgaacg cgtgccgcaa
     3841 gcagaccggc aagccaaagc tcgtgttcgc cagcagcgtg gcggtgttcg gcggcgaact
     3901 gcctgcggtc gtcagcgacg agacgcagcc cacgccgcag tcgagctacg gcgtgcagaa
     3961 gctggtctgc gaactgctga tcggcgactg ttcgcgcaag ggcttcatcg acggccgcag
     4021 cgtgcggctc ccgacgatcg tcgtgcggcc cggcaagccg aacgcggcgg cgtcgagctt
     4081 cgccagcggc atcgtccgcg aaccgctcgc gggcgagata tccgcctgtc cggtcgactc
     4141 gagcacggct atgtgggtgc tgtcgccgcg gtcggcgatc acggcgttcg ttcacgcgca
     4201 cgaactgccg gcggagcgat gggacgcgcg ccgcgcgctg aatctgcccg gcctgaccgt
     4261 gacggtagcg cagatgctgg aggcgctgcg cgcagtcggc ggcgacgccg cggtggcgct
     4321 cgtgaagatg cagcccgacg agcgcattgc cgccatcgtg cgcacgtggc cggcgcgctt
     4381 cgcgaccacg cgcgccgacg cgctcggctt cgcgcgcgac gccgacgtgg ggtcgatcgt
     4441 acgtgcctac gcgcgcgagt acgcggcggg tt
//
LOCUS       DBNHBY010000741         2765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1140910, whole genome shotgun sequence.
ACCESSION   DBNHBY010000741 DBNHBY010000000
VERSION     DBNHBY010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1140910"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="AC16HB_17405"
     CDS             complement(<1..685)
                     /locus_tag="AC16HB_17405"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR5608622.1"
                     /translation="MADSTGAGRKPRRSQAWFGRLDRDGFIYRSWTKNRGIPHDQFDG
                     RPVIGICNTWSELTPCNTHLRVLAEQVRYGVLEAGGFPLEFPVMSLGETLMRPTTMLF
                     RNLVSMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASVNLPTLGFSGGPMLSGKFR
                     GGELGSGTGVWQMSEAVRAGQMSQEDFFEAESCMHRSHGHCMTMGTASTMASMVEALG
                     MGLPGNAAIP"
     gene            866..1612
                     /locus_tag="AC16HB_17410"
     CDS             866..1612
                     /locus_tag="AC16HB_17410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5608623.1"
                     /translation="MNQLDLQGRCAVITGGAAGIGFAIAQRMLASGARVSLWDRDEQA
                     LANARGALGAPCDTRVVDVTREDRVGSARDATLAHFGRIDVLVCSAGITGPNLTVAEY
                     PPEAWRQVLDINLTGVFLCNRALVPPMRAANYGRIVNIASIAGKEGNPNASAYSASKA
                     GVIALTKSLGKELAGTNVRVNCVTPAAVKTGMFAQMTQQHIDFMLSKIPLGRFGAVEE
                     IAAMVAWLATEDCSFSTGAVFDLSGGRATY"
     gene            1620..2669
                     /locus_tag="AC16HB_17415"
     CDS             1620..2669
                     /locus_tag="AC16HB_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5608624.1"
                     /translation="MTRYRADDLRKFAGALLQRAGLPAARATIVADVLLEGDLLGHDT
                     HGLALLAAYLNELDRGAMRRDGEIEIVSDRPAVALWDGRRLPGPWLMVRAVDAALARA
                     RQLGSASIAIRRSHHIACLAAYLLRATEQRMVLLIASSDPNSASVAPYGGTRAVFTPN
                     PLAIGFPTAGEPVLIDVSASTTTNGMVARRHQAQTAFAHPWLLDAAGRPTADPAVMYS
                     DPPGTILPLGGLDAGHKGYGLALLIEALTGGLAGFGRADAKEGWGATIFVQALDPDAF
                     GGIEAFVRQTEHVARLCRENPPRDAAAPVRLPGERGLARRREQMANGVALHPSIMPAL
                     EPWAARLDTAVPATV"
BASE COUNT          421 a          978 c          947 g          419 t
ORIGIN      
        1 ccgggatcgc cgcattgccg ggcaggccca tgccgagcgc ctcgaccatc gaggccatcg
       61 tcgacgccgt gcccatcgtc atgcagtggc cgtgcgagcg gtgcatgcag ctttcggcct
      121 cgaagaaatc ctcctgtgac atctggcccg cgcgcaccgc ctcgctcatc tgccacacgc
      181 cggtaccgga gcccagttcg ccgccgcgga acttgcccga caacatcggt ccgccggaga
      241 agccgagcgt cggcaggttc acgctcgccg cgcccatcag cagcgccggc gtggtcttgt
      301 cgcagcccat caaaagcacc accccatcga gcgggttggc gcggatcgat tcctccacgt
      361 ccatgctgac caggttgcgg aacagcatcg tggtcggccg catcagcgtc tcgccgagcg
      421 acatcaccgg gaactcgagc ggaaagccgc ccgcttcgag cacgccatac ctgacctgct
      481 cggccagcac gcggaggtgc gtgttgcagg gcgtgagctc ggaccaggtg ttgcagatgc
      541 cgatcaccgg ccggccgtcg aactgatcgt gcggtatgcc gcggttcttg gtccaggagc
      601 ggtagatgaa gccgtcgcgg tcgagccgac cgaaccaggc ctggctgcgc cgcggcttgc
      661 gtcccgcgcc cgttgaatcc gccatgcgtt gtctcctcca gagcccttct cagcgacccc
      721 gagcgcggca gccgaggcgc gcaatctaaa cctcgccgcg cgcgccggtc aagccgaccg
      781 tggtcaagtg gtctggccaa ttgcagcgag ctcgcgtgat cgcggctata gtcgcgccgg
      841 ggaaatgacg cagagagaaa tcggcatgaa ccagctcgat ctgcaggggc gctgcgcggt
      901 gatcaccggt ggcgccgccg gtatcggctt cgcgatcgcg cagcggatgc tcgcgtcggg
      961 cgcgcgcgtg agcctgtggg accgcgacga gcaggcactc gcgaacgcac gcggtgcgct
     1021 cggcgccccg tgcgacacgc gcgtcgtcga cgtcacgcgc gaggaccggg tcgggtctgc
     1081 gcgcgatgcc acgctggcgc acttcggtcg catcgacgtg ctcgtgtgca gtgccggcat
     1141 caccggaccg aacctgaccg tggccgagta tccgcccgaa gcatggcggc aggtgctcga
     1201 catcaacctg acaggcgtct tcctgtgcaa tcgtgcgctg gtgccaccga tgcgtgccgc
     1261 caactacggc cgcatcgtca acatcgcttc gatcgccggc aaggaaggca acccgaacgc
     1321 cagcgcctac agcgcgtcga aggccggcgt gatcgcgctg accaagtcgc tcggcaagga
     1381 actcgccggc acgaatgtcc gcgtgaactg cgtgacgccc gccgccgtga agaccggcat
     1441 gttcgcgcag atgacgcagc agcacatcga cttcatgctg tcgaagattc cgctgggacg
     1501 gttcggcgcg gtggaggaga tcgcggcgat ggttgcgtgg ctcgcgaccg aggactgctc
     1561 gttctcgacc ggggcggtgt tcgacctgtc cggcgggcgc gcgacctact gacgcggcga
     1621 tgacgcgcta tcgcgccgac gacctgcgca aattcgcggg cgcgctgttg cagcgagcgg
     1681 gcctgcccgc cgcgcgcgcg accatcgtgg ccgacgtgct gctcgaaggc gacctgctcg
     1741 gccacgacac gcacgggctg gcgctgctcg ccgcgtacct gaatgaactc gaccgcggcg
     1801 cgatgcgacg cgacggcgaa atcgaaatcg tcagcgatcg gccggcggtg gcgctgtggg
     1861 acggtcgccg cctgcccgga ccctggctga tggtgcgcgc ggtcgacgct gcgctcgcgc
     1921 gggcgcgcca gcttggcagt gcgtcgatcg cgatccgccg cagccatcac atcgcgtgcc
     1981 tcgccgcata cctgctgcgc gccaccgagc aacgcatggt gctgctgatc gcaagctccg
     2041 accccaattc cgcgagcgtg gcgccctacg gcggcacgcg cgccgtgttc acgccgaacc
     2101 cgcttgcgat cggctttccg actgcgggcg agccggtgct gatcgacgtc tcggcgtcga
     2161 ccacgaccaa cggcatggtg gcgcgccggc accaggcgca gaccgcgttt gcgcatccgt
     2221 ggctgctcga tgccgccggc cggccgacgg ccgaccccgc ggtgatgtac tcggatccgc
     2281 ccggaacgat cctgccgctc ggcggactcg acgccgggca caagggctat ggcctcgcgc
     2341 tattgatcga ggctctgacg ggcgggctgg ccggcttcgg ccgcgccgat gcaaaggaag
     2401 gctggggtgc gacgatcttc gtgcaggcac tcgatccgga cgcgttcggc ggcatcgagg
     2461 cgttcgtgcg tcagaccgaa cacgtcgcgc gactctgccg ggagaatccg ccgcgcgacg
     2521 ccgccgcgcc ggttcgactg cccggcgagc gcggcttggc gcggcggcgc gagcagatgg
     2581 ccaacggcgt cgcattgcac ccttcgatca tgcccgcgct agagccgtgg gccgcgcggc
     2641 tcgacaccgc tgtgccagcg accgtctgag cgcgcgcgcg ccgcgcggaa gcaggtgttc
     2701 cgggcgctca cgccccggca cgtcaactta ctccctcctt cccggaagcg attcgacgcc
     2761 gtttc
//
LOCUS       DBNHBY010000742         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1342356, whole genome shotgun sequence.
ACCESSION   DBNHBY010000742 DBNHBY010000000
VERSION     DBNHBY010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1342356"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1423)
                     /locus_tag="AC16HB_17420"
     CDS             complement(116..1423)
                     /locus_tag="AC16HB_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR5608625.1"
                     /translation="MLTVVHDQESSNEKNDGIGRSLLDGIVRDGARQMLTAALLAEVA
                     AYVDAHAGEVDESGYRLVVRNGYHDERTVLTAAGAVAVKAPRVNDKRVDPQTGERQRF
                     SSAILPAWARKSPQMTEVLPLLYLHGLSSGDFAPALEAFLGTGAGLSASAITRLTVQW
                     QDEARAFAERDLSEVDYVYLWVDGIHLKVRLDQEKLCLLVMIGVRADGRKELVALTDG
                     FRESTESWTDLLRDCRRRGMRAPVLAVGDGALGFWKAVREVFPDTREQRCWWHKQANV
                     LAALPKSAHPGALAALKEIYNAEDIDKARVAIKAFEIDYGAKYPKAVAKIVADADVLL
                     EFYRYPAEHWIHLRTTNPIESTFATVRLRTKVTKGPGSRAAGLAMAYKLIEAAQTRWR
                     AVNAPHLVALVRNGAVFRKGKLLERPIDITPAEPDESHETEVA"
BASE COUNT          279 a          530 c          525 g          306 t
ORIGIN      
        1 ggagttcgat gtaggtagag cttgctgaag cggtgttgtg caggagatga gggattgttg
       61 ttggcccttc ggagtcgctc tgcgagatat tgtcaaaagc tgtggatggg gtgtttcagg
      121 cgacctccgt ttcgtgtgat tcgtcgggtt ctgctggtgt gatgtcgatg ggccgttcga
      181 ggagtttccc tttgcggaag acggcgccgt tgcggacgag ggcgaccagg tgtggtgcgt
      241 tgaccgcgcg ccagcgggtc tgtgcagcct cgatcagctt gtaggccatc gccaggccgg
      301 ctgcgcgcga tccgggtccc ttggtgacct tcgttctcaa acgtactgtg gcgaaagtgc
      361 tttcgatcgg gttagtcgtt ctcaggtgga tccagtgctc ggcggggtat cgatagaact
      421 cgagtagcac gtcggcgtcg gcgacgatct tggccaccgc cttggggtac ttggcgccgt
      481 agtcgatctc gaacgccttg atcgcgaccc gggccttgtc gatatcctcg gcgttgtaga
      541 tctctttgag cgccgcgagc gcaccgggat gcgccgactt cggcagcgcg gcaagaacat
      601 tggcctgctt gtgccaccag cagcgctgct cgcgggtgtc ggggaacacc tcccgcacgg
      661 ccttccaaaa ccccaacgca ccgtcgccga ccgcgagcac cggcgcccgc atgccgcggc
      721 ggcggcaatc acgcagcaga tcggtccagg attcggtgga ttcccggaag ccatcggtga
      781 gcgcgacgag ctccttgcgg ccgtcggcgc ggaccccgat catcaccagc aagcacaact
      841 tctcctggtc cagacgcacc ttcaggtgga tcccgtcgac ccacagatac acgtagtcga
      901 cctcggacag atcccgctcg gcgaacgcgc gggcctcgtc ttgccactgc acagtcagcc
      961 gggtgatcgc cgacgcggac agccccgcgc cggtgccgag gaacgcctcg agcgccgggg
     1021 cgaagtcacc cgaggacagc ccgtgcaggt acagcagcgg caacacctcg gtcatctgcg
     1081 gcgatttacg agcccacgcc ggcaaaatcg cggaggaaaa tcgttgccgt tcaccggttt
     1141 gcgggtcgac gcgcttgtcg ttgacccgcg gagctttcac cgccacggcg cccgccgcgg
     1201 tcagcacggt ccgctcgtcg tggtagccgt tgcggaccac cagccgatac ccgctctcgt
     1261 cgacctcacc ggcatgggca tcgacgtagg cggcgacctc ggccagcaac gcggccgtga
     1321 gcatctgccg ggcaccatcg cgaacgatcc catcgagcaa tgaccgaccg ataccgtcgt
     1381 tcttctcgtt ggatgattcc tggtcgtgaa ctaccgtgag catgggcgta ccttcccgaa
     1441 ccagcgcgcc accgccggct cttgatcaga ccaactggga ctttcagatc atcctcggga
     1501 aggtgcgccc gttcgcgtca ccaggccgag ggccatccac aggttctgat cattgctctt
     1561 gggtgagtgt gccaacgaac accagccgac acgcggcgga cacaaccgcg ctagaaaccc
     1621 gtcccgcacc ccgtagccgg
//
LOCUS       DBNHBY010000743         1573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_124196, whole genome shotgun sequence.
ACCESSION   DBNHBY010000743 DBNHBY010000000
VERSION     DBNHBY010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_124196"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1154)
                     /locus_tag="AC16HB_17425"
     CDS             complement(183..1154)
                     /locus_tag="AC16HB_17425"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5608626.1"
                     /translation="MNRDPASGAASRVNPALRAIVVLLAAAAALYLALALYVDWRELA
                     HAIGQLGFFALCGGLLLASLNYLLRFARWHDILRRMGQRVPGSANLRIYVGGLALTAT
                     PGKLGETLRSALLLRWQVPVGASLAAFFIDRLTDLIGVLVLAGVSGGGAFWWAIAALA
                     LAVGVVLRHAFGTAWAGAGADWLERHHRLTALAVLLRTGMTHYRAAWRLPHVAAYVLV
                     AVIAYGVQALVFAAYVETLWPGSRWADSVRIFATSTLAGAASMIPGGLGAMELALIAQ
                     SMAAGMPLSSATAAALAVRAVTLWFAILLGLACLLSLRRQFAVASTS"
     gene            complement(1151..>1573)
                     /locus_tag="AC16HB_17430"
     CDS             complement(1151..>1573)
                     /locus_tag="AC16HB_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR5608627.1"
                     /translation="WPWKTEVAESIAESRRFALFLVAGGLAALANYGSRFGFSLWFSY
                     PVAIVCAYLVGMAVAFALMRQYVFDGRGKAIGPQVTRFVGVNVLAVLQTLVVSLLLAR
                     WLLPAAGVETHAEAIAHAVGVAVPVVTSYFGHRLATFR"
BASE COUNT          317 a          576 c          503 g          177 t
ORIGIN      
        1 tgttcggcga tgcgcgcctt gaacctcgcc cggcctccgg ccagccacac cggcagtgca
       61 aacgcttgcc atggctggcg cttgagcagg tcgatgatcg actcgacgag cgtgtcggaa
      121 cggatcagag tgccgtcgag atcgatgcaa agcggaacca ggggacgatc cggcacgatg
      181 gatcaggagg tggatgcaac ggcgaactga cgccgcagcg acagcagaca agcgagcccg
      241 agcaggatag caaaccaaag cgtgacggcc cgcaccgcaa gcgcagccgc cgtcgccgac
      301 gacaacggca ttccggcggc catcgactgc gcaatcagcg cgagttccat ggctccgagg
      361 ccacccggaa tcatcgatgc agcgcctgcc aacgtcgacg tcgcgaagat gcggaccgag
      421 tccgcccagc ggctccccgg ccacagggtc tcgacgtacg ccgcgaacac cagcgcctgc
      481 acgccgtagg cgatcactgc gacgaggacg tacgccgcca cgtgcggcag ccgccacgcc
      541 gcgcgatagt gcgtcatccc ggttcgcagc agcacggcga gcgccgtcag gcgatggtgt
      601 cgttcgagcc aatcggcccc ggcaccggcc cacgccgtgc cgaacgcgtg ccgcaggacg
      661 actccgaccg cgagcgccag tgcggcgatc gcccaccaga atgctcctcc gccactgacc
      721 ccggcgagca ccagcacgcc gatcaggtcc gtcaaccgat cgatgaagaa ggccgcgaga
      781 ctcgcgccga ctggcacctg ccagcgcagc agcagcgcgc tgcgcagcgt ctcgccgagc
      841 ttgcccggcg tcgccgtcag cgcgagcccg cccacgtaga tgcgcaggtt cgccgaccca
      901 gggacgcgtt gacccatccg tcgcaggatg tcgtgccagc gcgcgaaccg cagcaggtag
      961 ttcagcgacg cgagcagcaa tccgccgcac agcgcaaaga agccaagttg gccgatcgcg
     1021 tgcgccagct cgcgccagtc gacgtacagc gcgagcgcaa ggtacagcgc ggccgccgca
     1081 gccagcagca caacgatcgc gcgcaacgcc gggttcactc gggacgccgc gccggacgcc
     1141 ggatcgcggt tcaccggaac gtcgccagcc ggtgtccgaa ataactggtc accacaggca
     1201 ccgcgacacc gaccgcgtgc gcaatcgcct cggcgtgggt ctcgacgccc gcggccggca
     1261 gcagccagcg cgccagcaac aggctgacga ccagcgtctg cagcacggcg agcacgttca
     1321 cgcccacgaa gcgcgtgacc tgcggtccga tcgccttgcc gcggccatcg aacacgtact
     1381 ggcgcatcag cgcgaacgcg acggccatcc cgaccagata ggcgcacacg atcgccaccg
     1441 gatagctgaa ccagaggctg aacccgaagc gcgatccgta attggcgagc gcggccagcc
     1501 cgccggcaac tagaaacaac gcaaagcggc gcgattcggc gatcgactca gccacttccg
     1561 tcttccatgg cca
//
LOCUS       DBNHBY010000744         4652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1815145, whole genome shotgun sequence.
ACCESSION   DBNHBY010000744 DBNHBY010000000
VERSION     DBNHBY010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1815145"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC16HB_17435"
     CDS             complement(<1..462)
                     /locus_tag="AC16HB_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HIR5608628.1"
                     /translation="MKELRHAALDALLIADPAAKCAAVVAINAQERPVDAAVRIDPPD
                     AVALPGRPVLPRLVPPAQVADRGSGTAAGRAALLHALAHIEFNAINLALDAVWRFANL
                     PAEFYRDWLKVAAEEGRHFALLCGRLAACGARYGDFDAHDGLWDMAYKTRHD"
     gene            complement(459..1127)
                     /locus_tag="AC16HB_17440"
     CDS             complement(459..1127)
                     /locus_tag="AC16HB_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5608629.1"
                     /translation="MKPRRTLVDQAYDAILDAICDGSLAPGARVTQDELATRLHVSRQ
                     PVLTALGLLKQQGFLIDHGRRGLAVVPVDPSRFEAIYQIRSALEPLAAKLATRHMTPQ
                     LIEHGRAIIALGRKVAAAGDAKATLQADVDFHNFIYEASGNPLIVETMQLHWQHLRRA
                     MGEVLRRPRMTQQVWREHAAVLEAMAAGDGATAARLLQQHVDGARVRVGEEIAAMEQE
                     RKAA"
     gene            1255..1662
                     /locus_tag="AC16HB_17445"
     CDS             1255..1662
                     /locus_tag="AC16HB_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5608630.1"
                     /translation="MSTPIRVLLAKVGLDGHDRGVKVVARTLRDAGMDVIYSGLHRTP
                     EEVVNTAIQEDVDVLGVSLLSGVQMTVFPKLFRLLDERGASDIIVVAGGVMPDEDAAA
                     IKKMGVREVLLQDTPPQVIVDTLHRLVEQRGPR"
     gene            1672..3105
                     /locus_tag="AC16HB_17450"
     CDS             1672..3105
                     /locus_tag="AC16HB_17450"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5608631.1"
                     /translation="MEQWSFPPRYDDDYLPPAGSRYWFPVRETMPAAERERAILARLQ
                     EVCRHAYEHAPFYRRQWDEAGFHPSQLKSLEDFETRVPVVKKADLRASQSAYPPFGDY
                     LCVPDEEIFHIHGTSGTTGRPTAFAIGRNDWRAIANAHARIMWGMGMRPGDMICVAAI
                     FSLYLGSWGALAGAERLRAKAFPFGAGAPGMSARLAQWLHTMRPAGFYGTPSYAIHLA
                     EVAREEKLHPRDFRIKYMFFSGEPGASVPGVRDRIEDLYGARVFDCGSMAEMSPFMNV
                     AGSAETHHGMLCWQDVVYTEVCDPANLRRVPYSQRGTPVYTHLERTSQPMIRLLSGDL
                     TLWVNEENPCGRTYPRLPQGVFGRIDDMFTIRGENIYPSEIDAALNQLAHYGGEHRIV
                     ITRDAAMDELLLRVEADDPTHRNPAAQTAFRDEVARKLQTVLGIRTKVEVLAPHALPR
                     TDFKARRVIDDREVFRSLNAELARSRE"
     gene            3102..4088
                     /gene="meaB"
                     /locus_tag="AC16HB_17455"
     CDS             3102..4088
                     /gene="meaB"
                     /locus_tag="AC16HB_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR5608632.1"
                     /translation="MKYVPSSELVEDIARSDVRAAGRLMSRAEAGAEEARAALAEIYR
                     RAGAAHVIGLTGVPGSGKSTLVAKVTHALRARGEKVGIVAVDPSSPYSGGAILGDRIR
                     MSELANDPGVFIRSMATRGATGGMARAALDTVDILDVAGYSTVIVETVGVGLDEVEIA
                     HASDTTIVVSAPGLGDEIQAIKAGILEIADIHVVSKCDRDDANRTLTDLKHMLTLGAL
                     TKDKPLWQIPVVGVSSYTGQGIDDLIGRIDAHRTTLKTSALGAQRRARVAAFRLAKTA
                     ETVLLEGFRTAAATLSAPLAQRLAQRASDPYSLARELINDTLRKEYSHELAR"
     gene            4072..>4652
                     /locus_tag="AC16HB_17460"
     CDS             4072..>4652
                     /locus_tag="AC16HB_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5608633.1"
                     /translation="MNWLDEAAIAKLREQERRWEKDEVAAFLKKQAEKREQFFTTGDV
                     PVKRVYTAADVAATPLEDIGLPGQYPFTRGPYPTMYRSRTWTMRQIAGFGTGEDTNKR
                     FKYLIAQGQTGISTDFDMPTLMGYDSDHPMSDGEVGREGVAIDTLADMEALLDGIDLE
                     KISVSLTINPTAWILLAMYVALAEKRGYDLAKLS"
BASE COUNT          784 a         1611 c         1557 g          700 t
ORIGIN      
        1 gtcgtgccgc gtcttgtacg ccatgtccca cagcccgtca tgggcgtcga agtcgccgta
       61 gcgcgccccg cacgccgcca gccggccgca cagcagcgcg aagtgacgcc cctcctcggc
      121 cgcgaccttg agccagtcgc gatagaactc ggccggcaga ttcgcgaagc gccacacggc
      181 atcgagcgca aggttgatcg cattgaactc gatatgcgcc aacgcgtgca gcagcgcggc
      241 gcgaccggcc gcagtgccgc taccccgatc ggcgacctgc gccggcggga cgagccgcgg
      301 cagcacgggg cgaccgggta acgcgactgc gtccggcggg tcgatgcgca cggccgcatc
      361 gaccggccgc tcttgcgcgt tgatcgccac cacggccgcg cacttggccg cgggatcggc
      421 gatcagcagc gcgtccagtg ctgcgtgccg cagttccttc acgccgcctt gcgctcctgt
      481 tccatcgcgg caatctcctc gccgacgcgc acgcgcgcgc cgtcgacgtg ctgctgcagc
      541 agccgcgccg cggtcgcacc gtcgccggcg gccatcgcct cgagcacggc cgcgtgttcg
      601 cgccacacct gctgcgtcat gcgcggccgg cgcagcacct cgcccatcgc gcggcgcaag
      661 tgctgccagt gcagctgcat cgtctcgacg atcagcggat tgccgctggc ttcgtagatg
      721 aagttgtgga aatcgacgtc ggcctgcagc gttgccttgg cgtcgccggc ggcagcgacc
      781 ttgcgcccga gcgcgatgat cgcgcgcccg tgctcgatca gctgcggcgt catgtgccgc
      841 gtggcgagct tcgccgccag cggttcgagc gcggaacgga tctggtagat cgcttcgaag
      901 cgcgacggat cgaccggcac gaccgccagg ccgcgccggc catgatcgat caggaagccc
      961 tgctgcttga gcaggcccag cgccgtcagc accggctggc gcgacacgtg caggcgcgtc
     1021 gcgagttcgt cctgcgtaac gcgcgcgcct ggcgccagcg agccatcgca aatcgcgtcc
     1081 aggatcgcgt catacgcctg gtcgaccaac gttctccggg gtttcaatgc tcgcatcacg
     1141 tgtatccgtc cttcccgtcg ttgacagcat gccccgatcg ctgccacact ttttatcgta
     1201 taccgtatac gaatatttga caagaagcct cccctcgacc cggacccaca cccaatgagc
     1261 accccgattc gcgtactcct tgccaaggtc ggcctcgatg gccacgaccg cggcgtcaag
     1321 gtcgtcgcgc gcacgctgcg cgacgccggc atggacgtca tctactcggg cctgcaccgc
     1381 acgccggagg aggtcgtgaa caccgcgatc caggaggacg tcgatgtcct cggcgtgagc
     1441 ctgctgtcgg gagtgcagat gacggtgttc ccgaagctct tccgcctgct cgacgagcgc
     1501 ggcgccagcg acatcatcgt cgtcgccggc ggcgtgatgc cggacgagga cgccgccgcg
     1561 atcaagaaga tgggcgtgcg cgaagtgctg ctgcaagaca cgccgccgca ggtgatcgtc
     1621 gacacgctgc atcgcctggt cgaacaacgc ggaccgcgct gaggagcgtc gatggaacag
     1681 tggtcctttc cgccccgcta cgacgacgac tatcttccgc cggccggcag ccgctattgg
     1741 tttccggtgc gcgaaacgat gcccgccgcc gagcgcgagc gcgcgatcct ggcgcggctg
     1801 caggaggtgt gccgccacgc gtacgagcac gcgccgttct atcgccgcca gtgggacgaa
     1861 gccgggtttc atccgagcca gttgaagtct ttggaggact tcgaaacgcg cgtaccggtg
     1921 gtcaagaagg ccgacctgcg cgcgtcgcag tcggcgtatc cgccgttcgg cgattacctg
     1981 tgcgttcctg acgaagagat cttccatatc cacggcacca gcggcacgac gggacgcccg
     2041 accgcgttcg cgatcggccg caacgactgg cgcgcgatcg ccaatgcgca cgctcgcatc
     2101 atgtggggca tgggaatgcg ccccggcgac atgatctgcg tggccgcgat cttcagcctg
     2161 tacctcggca gctggggcgc actggcggga gccgagcggc tgcgcgcgaa ggcgtttccg
     2221 ttcggcgccg gcgcgccggg gatgagtgcg cggctcgcgc aatggctgca cacgatgagg
     2281 ccggccggct tctacggcac gccgagctac gcgatccacc tggccgaggt cgcgcgcgaa
     2341 gagaagctgc atccgcgaga cttcaggatc aagtacatgt tcttctcggg ggaaccgggc
     2401 gcgtcggtac cgggcgtgcg cgaccgcatc gaggacctgt acggcgcgcg cgttttcgac
     2461 tgcggctcga tggccgagat gtcgccgttc atgaacgtcg ccggctcggc agaaacgcac
     2521 cacggcatgc tgtgctggca ggatgtcgtc tacaccgagg tgtgcgatcc ggcgaacctg
     2581 agacgagtgc cctacagcca gcgcggcacg ccggtgtaca cgcacctgga acgcaccagc
     2641 cagccgatga tccggctgct gtcgggcgac ctgacgctgt gggtcaacga agagaatccg
     2701 tgcggccgca cgtacccgcg gctgccacag ggcgtgttcg ggcgcatcga cgacatgttc
     2761 acgatccgcg gcgagaacat ctacccgagc gagatcgacg cggcgctgaa ccagctcgcg
     2821 cactacggcg gcgagcaccg catcgtgatc acgcgcgacg cggcgatgga cgagttgctg
     2881 ctgcgcgtgg aagccgacga ccccacgcac cgcaacccgg ccgcgcagac cgcgttccgc
     2941 gacgaggtcg cgcgcaagct gcagaccgtg ctcggaatcc gtaccaaggt cgaggtgctc
     3001 gcgcctcatg cgcttccgcg caccgacttc aaggcgcggc gcgtgatcga cgaccgtgag
     3061 gtgttccgct cgctgaatgc cgaactggcc cggtcgcgcg aatgaaatac gtcccatcat
     3121 cagagctcgt cgaggacatc gcacgcagcg acgtgcgcgc cgccggccgc ctgatgtcgc
     3181 gcgccgaagc cggcgccgag gaggcgcgcg cggcgctggc cgagatctat cgccgcgccg
     3241 gcgcggcgca cgtgatcgga ctgaccggcg tgcccggcag cggcaagtcg acgctggtcg
     3301 cgaaggtgac gcacgcgctg cgcgcgcgcg gcgagaaggt cggcatcgtc gcggtcgatc
     3361 cgtcgagccc gtactccgga ggcgcgatcc tcggcgaccg catccgcatg tcggaactgg
     3421 ccaacgatcc cggtgtcttc atccgcagca tggccacacg cggcgccacc ggcggcatgg
     3481 cgcgggcggc actcgacacg gtcgacatcc tcgatgtcgc tggctattcg acggtcatcg
     3541 tcgagaccgt cggcgtcggc ctggacgaag tcgagatcgc gcacgcatcc gacaccacga
     3601 tcgtcgtgtc ggcgccgggg ctcggcgacg agatccaggc gatcaaggcc gggatactgg
     3661 agatcgccga cattcacgtg gtttcgaagt gcgatcgtga cgacgccaat cgcaccctga
     3721 cggatctcaa gcacatgctg acgctcggcg cgctgacgaa ggacaagccg ctgtggcaga
     3781 tccccgtggt cggcgtcagt tcttacaccg gccagggcat cgacgacctg atcggccgca
     3841 tcgacgccca ccgcacaacg ctgaaaacct ccgcgctcgg cgcgcagcgc cgcgcgcgcg
     3901 tggccgcgtt tcgcctcgcc aagacggctg agacggtgct gctcgaaggc ttccgaaccg
     3961 ctgccgcaac gctctccgcg ccgctcgcgc aacggctggc acagcgagcg tcggacccct
     4021 attcgctcgc gcgcgagctg atcaacgaca cgctacgcaa ggagtattcg catgaactgg
     4081 ctcgatgagg ccgccatcgc caagctgcgc gagcaggaac gccgctggga aaaggatgaa
     4141 gtcgccgcct tcctgaagaa gcaggccgag aagcgtgaac agttcttcac gaccggcgac
     4201 gtgccggtca agcgggtcta caccgcggcg gacgtggcgg ccacgccgct ggaggacatc
     4261 gggctgcccg gccagtatcc gttcacgcgc gggccgtacc cgacgatgta ccgcagccgc
     4321 acgtggacga tgcggcagat cgccggcttc ggcaccggcg aggacaccaa caagcgcttc
     4381 aagtacctga tcgcgcaggg gcagaccggc atttcgaccg acttcgacat gcccacgctg
     4441 atgggctacg actcggatca cccgatgagc gacggcgagg tcggccgcga gggcgtggcg
     4501 atcgacacgc tggcggacat ggaagcgctg ctcgacggca tcgacctcga gaagatttcg
     4561 gtgtcgctga cgatcaaccc gaccgcgtgg atcctgctcg cgatgtacgt ggcgctggcc
     4621 gagaagcgcg gctacgacct cgccaagctg tc
//
LOCUS       DBNHBY010000745         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1141365, whole genome shotgun sequence.
ACCESSION   DBNHBY010000745 DBNHBY010000000
VERSION     DBNHBY010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1141365"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16HB_17465"
     CDS             <1..780
                     /locus_tag="AC16HB_17465"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR5608634.1"
                     /translation="FYYAGGLPAVIRELGPVLDKDALTVNGKTIWDNNKDAPRYDLEG
                     GKRVIRPIDDPFKPRAGIAVLRGNLCPDGAVLKPLAATPALMKHRGRAVVFESSEDMH
                     AKVEDETLDVKPDDILVLKNCGPKGYPGMAEVGNMPIPVKLVKQGVTDMVRISDARMS
                     GTAYGTVVLHGAPEAAAGGTLALVKNGDIIELDVERRRLHLEVSDEELARRRAAWTPP
                     AAPMPRGYVKLYFDHVLQANQGADLDFLVGASGSAVAKDNH"
     gene            820..1788
                     /locus_tag="AC16HB_17470"
     CDS             820..1788
                     /locus_tag="AC16HB_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR5608635.1"
                     /translation="MKALITDHDFPDLELERGIYREAGIELAVAHCKSEDALIAAARD
                     CDALLVQYAPVTARVLQALPRIGLVSRYGAGYDTIDTAACARHGVWVANSPDYGVGEV
                     ATHALAMALALIRHLAIYDRDVKAGRWHYLSPGTIRRASDMTVGILGLGRIGKRFAHL
                     ARNSFARVIACDPYIIDGDFPAYVERVDLPRLFERADLVSLHVPLTDETRGMVNATLL
                     ERMQSGSWIVNTARGAVVDIDDLLAALNDGTLDGAALDVLPTEPLAADHPLARHPRAL
                     LSPHAAFFSTDAETELRRKAALNIVQWARTGRPMYPVVQGTRSLPR"
BASE COUNT          313 a          633 c          624 g          272 t
ORIGIN      
        1 ttctactacg cgggcggcct gccggcggtg atccgcgaac tcggtccggt gctcgacaag
       61 gacgcgctca cggtcaacgg caagaccatc tgggacaaca acaaggacgc gccgaggtac
      121 gacctcgaag gcggcaagcg cgtgatccgg cccatcgacg atccgttcaa gccgcgggcc
      181 ggcatcgcgg tgctgcgcgg caatctgtgc cccgacggcg cggtgctcaa gccgttggcg
      241 gccacgcccg cgctgatgaa gcaccgcggt cgcgcggtcg tgttcgagtc gagcgaggac
      301 atgcacgcca aggtcgagga cgaaacgctc gacgtgaagc ccgacgacat cctggtgctg
      361 aagaactgcg gacccaaggg ctaccccggc atggccgagg tcggcaacat gccgattccc
      421 gtcaagctcg tgaaacaggg cgtgaccgat atggtgcgca tctcggacgc ccgcatgagc
      481 ggcaccgcgt atggcacggt cgtgctgcac ggcgcgcccg aggccgccgc cggcggcacc
      541 ctcgcgctgg tgaagaacgg cgacatcatc gagctcgacg tcgaaaggcg ccgactccat
      601 ctcgaagtct ccgacgagga actcgcgcgc cgccgcgctg cctggacgcc gcccgccgcg
      661 ccgatgccgc gcggctatgt gaagctgtac ttcgatcacg tactgcaggc gaaccagggc
      721 gccgacctcg acttcctcgt cggcgcgagc ggctctgccg tggccaagga caaccactga
      781 aatcgatgtg ccggatcacc gtgctcattc acggttgcca tgaaagccct gatcaccgac
      841 cacgactttc ccgacctcga actcgagcgc ggcatttacc gcgaggcggg gatcgaactg
      901 gcggtcgcgc actgtaagag cgaagacgcc ctgatcgcgg cggcgcgcga ctgcgatgcg
      961 ctactggtgc agtacgcgcc ggtcacggcc cgcgtgctgc aggcgctgcc tcggatcgga
     1021 ctcgtcagtc gctacggggc gggctacgac acgatcgaca cggccgcatg cgcccggcac
     1081 ggcgtgtggg tcgccaactc gcccgactac ggggtgggcg aagtggccac gcatgcgctg
     1141 gcgatggcgc tggctctgat caggcacctc gcgatctacg accgcgacgt caaggccggc
     1201 cggtggcact acctgtcgcc tggcacgatc cgccgtgcga gcgacatgac ggtcggcatc
     1261 ctcgggctgg gccgcatcgg caagcgcttc gcgcacctgg cgcgcaactc gttcgcgcgt
     1321 gtcatcgcgt gcgatccgta catcatcgac ggcgacttcc cggcgtatgt cgagcgggtg
     1381 gacctgccgc ggctcttcga gcgcgccgat ctcgtatcgc tgcacgtgcc gctgaccgac
     1441 gaaacgcgcg gcatggtcaa tgcgactctg cttgagcgca tgcagtccgg aagctggatc
     1501 gtcaacaccg cgcgcggcgc ggtggtcgat atcgacgacc tgctcgcggc gctgaacgac
     1561 ggcacgctcg acggcgcggc gctcgacgtg ctgccgacgg agccgctcgc cgccgaccac
     1621 ccgctggcgc gccatccgcg cgcgctgctg tcgccgcatg cggcgttctt ctcgaccgac
     1681 gccgagacgg aacttcggcg caaggcggcg ctcaacatcg tgcaatgggc gcgcaccggg
     1741 cgccccatgt atcctgtcgt gcaagggaca aggagccttc cccgatgaag gccggatcag
     1801 gagacagatc gagatggcac gggtcgcgat tcgcggcgta gc
//
LOCUS       DBNHBY010000746         3453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_665099, whole genome shotgun sequence.
ACCESSION   DBNHBY010000746 DBNHBY010000000
VERSION     DBNHBY010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_665099"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1019
                     /locus_tag="AC16HB_17475"
     CDS             63..1019
                     /locus_tag="AC16HB_17475"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR5608636.1"
                     /translation="MARRRKTTEPAALQWPWQVNAGIGVVGLAACYWLPTLMFKGPLA
                     LAFIPMFRVIGWAIFLVFGLLSLVVFLRMRGAERAPESSGNERREPTMRKTATSEAAP
                     SRTKLDDTWDEQLRSAARNRTAPAAAPPSIEAWSMDVLRRMEWKRFEIVAAAYYRALG
                     FRPETIRCGADGGIDIKLFRGDLPDAISLVQCKAWTSRPVGVKPVRELLGVMTNERVK
                     AGVFLTTNIYTAEAVRFADGNKLALIDGEQFLQKLTALPAAAASELLRVATEGDWTTP
                     SCPSCGVKMVLREGRQKSFWGCPNFPRCRQTFFYSEGERDSD"
     gene            1084..1782
                     /locus_tag="AC16HB_17480"
     CDS             1084..1782
                     /locus_tag="AC16HB_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5608637.1"
                     /translation="MPSLAHQLFGQTRSAVLSALLLHPEASLHVRELARLTGASAGSL
                     HRELRTLAELGLLLRQEVGRQVHYRANPAHPVHAELSQLLRKTAGLADVLREALLPLG
                     AKVELAFVYGSVAAGAERAGSDVDLMVLGQATFADLARALAHAQSILRRDVNPTVMTR
                     REFARGLAAGNGFVRSLMKSEKLWLKGSDDDLAEPVEDRAPEATRTQRGRGAKAARRD
                     QAQPVRRRGRRHQR"
     gene            1715..2104
                     /locus_tag="AC16HB_17485"
     CDS             1715..2104
                     /locus_tag="AC16HB_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIR5608638.1"
                     /translation="MQRLLAAIKRNLSDAAVAGISDETRFDVAYKAVMQCALVAMMAA
                     GYRPSANEPGHHQTMIQSLPLTLGVENDTWVVLDALRRQRNANDYTGQPIMPAAVAEC
                     LNQARTLEKALLAHLRSKHPELLAPAA"
     gene            2170..2964
                     /locus_tag="AC16HB_17490"
     CDS             2170..2964
                     /locus_tag="AC16HB_17490"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR5608639.1"
                     /translation="MLLLSPFILVLALAASWPASAQTVFRCVGGDGKVGYQAGPCDER
                     SKQSEVRIRVDPPDPAAAPKKYRWQGYKPPKVAALTFYYDPAEEPVGFSTAQMEADIH
                     AAIAAWMSGCDVQLHYGGRRPARPPGTPDNVPIRWAPEYMRMAHPADGRSGIAGSGSL
                     AGGIALKPRFHEAHMRSVLIHEMGHVLGLAHNHDDPQSVMSYLRDEGAQRNPQPSPSD
                     FLACNLSMKRQFGIDFKPPPDAPAPPQGLRMSDREALEKIHGPAPR"
     gene            3299..>3453
                     /locus_tag="AC16HB_17495"
     CDS             3299..>3453
                     /locus_tag="AC16HB_17495"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5608640.1"
                     /translation="MTITDRIELNPKIMLGKPVIRGTRITVELIVRKLAEGADEATLL
                     AAHPGLT"
BASE COUNT          600 a         1104 c         1146 g          603 t
ORIGIN      
        1 tggtcgatga gcgcaatgat ttgaactgcc atctcgtgaa caggtgagga gcgtcgtcat
       61 cgatggctcg acgcaggaag accactgaac cagccgcgct gcaatggcca tggcaagtga
      121 atgccggcat tggcgtcgtg ggcttggccg cctgctattg gcttcccacc ttgatgttca
      181 agggaccgct cgcgctggcc ttcatcccga tgttccgcgt gattggctgg gccattttct
      241 tggtgttcgg gctgctttcg ctcgtcgtat tcctgcgcat gaggggtgcg gagcgggcgc
      301 ccgagtcgtc gggaaacgaa aggcgtgagc cgaccatgcg caagacggcc acgtcggagg
      361 cggcgccctc gcgtaccaag ctcgacgaca cctgggacga gcagctcaga tcggcggcgc
      421 gaaacagaac cgctcccgcc gccgcaccgc catccatcga agcgtggtcg atggacgtac
      481 tgcgccgcat ggagtggaag cgcttcgaga tcgtcgctgc ggcctactac cgagcgctgg
      541 gcttccggcc ggagacgatt cgctgcggcg ccgacggtgg aatcgacatc aagctatttc
      601 gcggtgatct tccggatgcg atctcgctcg tccaatgcaa ggcgtggact tcgcgacccg
      661 tgggcgtcaa gcctgttcgg gagctgctgg gcgtcatgac gaatgaaagg gtgaaggctg
      721 gcgtcttcct cacaaccaac atctatacgg cggaggcggt gcgattcgcc gacggcaaca
      781 agctggcctt gatcgacggc gagcaattcc tccagaaact gaccgcgttg ccggccgcag
      841 ctgcgtccga gctcttgcgc gtggcgaccg agggcgattg gacgacaccg tcatgtccgt
      901 cgtgtggcgt gaagatggtc ctgcgcgaag gtaggcagaa gtccttctgg ggctgtccca
      961 actttccgcg ttgtcggcag acgttcttct actccgaggg ggaaagagac agcgactgat
     1021 accggcacgc aggtggcgcc atgatgttct caaaccggct tcatttgtag caaaatacgc
     1081 tacatgccat cgctggccca tcaattgttc ggccaaactc gctcggcggt cctgagtgcc
     1141 ttgctgctgc accccgaggc ctcgctgcac gtgcgcgagc tcgcacgcct gacgggcgct
     1201 tcggccggct cgctgcaccg cgagttgcgt accctggccg aactcggcct gctgctgcgg
     1261 caggaggtcg ggcgacaggt gcactaccgc gcgaaccccg cgcacccggt gcatgccgag
     1321 ctgtcgcagc ttctgcgcaa gacggccggc ctggcggacg ttctgcgcga agcgctgttg
     1381 ccgttgggcg ccaaggtgga gttggccttc gtctatggca gcgttgccgc tggcgctgag
     1441 cgcgctggca gcgacgtgga cctgatggtg ctgggacagg cgacgtttgc cgatctggcg
     1501 cgcgcgctgg cgcatgcgca atccatcctg cggcgcgacg tgaacccgac cgtcatgacg
     1561 cggcgcgagt tcgcgcgcgg gctggctgcc ggcaacggct tcgtgcgcag cctgatgaag
     1621 agcgagaaac tctggctcaa gggaagcgat gatgaccttg cagaacctgt tgaagatcgg
     1681 gcgcctgaag ccacacgaac ccaacgcggc cgaggtgcaa aggctgctcg ccgcgatcaa
     1741 gcgcaacctg tccgacgccg cggtcgccgg catcagcgat gagacgcgct tcgacgtcgc
     1801 ctataaggcg gtgatgcagt gcgccttggt ggcgatgatg gccgcgggct accgaccctc
     1861 ggccaacgag cccggtcatc accagaccat gattcaatcg ctgccgctca cgctcggcgt
     1921 ggaaaacgac acgtgggtgg tgctcgatgc gctgcgccgc caacgcaatg cgaacgacta
     1981 caccgggcag ccgatcatgc ccgcggccgt cgccgagtgc ctgaatcagg cgcggacgct
     2041 cgagaaagct ttgctcgccc atctgcgatc gaagcatccg gaattgctcg ctccggctgc
     2101 atgaacgcgt tcgcccttgt ccggagacgt cgggaccacc gcaatgtcct gcggggcgat
     2161 cgtcgcgccg tgctgctcct ttcgccgttc atcctcgttc tggcgctcgc ggcctcgtgg
     2221 ccggcatcgg ctcagaccgt gttccgttgc gtgggtgggg acggcaaggt cggctaccag
     2281 gcaggaccct gcgacgagcg ctcgaagcaa agcgaagttc gcattcgcgt cgacccgccc
     2341 gaccccgctg ccgcgccgaa gaagtatcgc tggcagggct acaaaccgcc caaggtcgcc
     2401 gcgctgacct tctactacga cccggccgag gagccggtcg gtttctccac tgcgcagatg
     2461 gaggcggata ttcatgcggc catcgcggcc tggatgagcg gctgcgacgt gcagcttcac
     2521 tacggcggac gccgcccggc acgcccgccc ggcacgccgg acaacgtgcc gatccgctgg
     2581 gcgcccgagt acatgcgcat ggcgcacccg gcggacggtc gcagcggaat cgcgggcagc
     2641 ggcagcctcg caggcggcat cgcgctgaag ccccgctttc acgaagcgca catgcgatcg
     2701 gtcctgatcc atgagatggg tcacgtgctg gggctggcgc acaaccatga cgacccgcag
     2761 tcagtcatga gctacctgcg cgacgagggc gcacagcgca atccgcagcc ctcgccgtcg
     2821 gacttccttg cctgcaacct ctccatgaag cggcaattcg gcatcgactt caagccgcca
     2881 cccgacgcgc cggcgccgcc gcaaggcttg cgcatgagcg accgcgaggc gctggagaag
     2941 atccacggcc cggcgccgcg ctaggtggga agagtcaagg ccgcgcgcgg tgcgctgggc
     3001 agctacttcg tctcgcggtt gtcggacgcg gagcggggga tcgggaaact cgagggttgg
     3061 attctggggg tcaggtagct tcggcagcgg gccgctggct gcaggtgcaa gttctgtctt
     3121 ctgctgtggt cacgttttgt gaacgattgg agggcttgaa cgcacgggca ggttcgtcgc
     3181 tcgcgacaag cccagtcttg atatcttctg gctcaatgac aaacgcctcg ccgacagcga
     3241 caacccgccc gcgccggaag tgattgcgca gtagagcgtt gacgacctgg aggccgctat
     3301 gaccatcact gaccgcatcg aactcaaccc gaagatcatg ctgggaaagc ctgtgatccg
     3361 cggcactcgc atcactgtgg agctcatcgt tcgcaagctt gccgaaggcg ccgacgaggc
     3421 aacgcttctc gcggcgcatc cgggtctcac aag
//
LOCUS       DBNHBY010000747         6598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_124468, whole genome shotgun sequence.
ACCESSION   DBNHBY010000747 DBNHBY010000000
VERSION     DBNHBY010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_124468"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..3474
                     /locus_tag="AC16HB_17500"
     CDS             19..3474
                     /locus_tag="AC16HB_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR5608641.1"
                     /translation="MAIHVALNHVTHYRYDRRVGLSPQVVRLRPAPHTRTPILAYSLR
                     VEPAGHFVNWQQDPFSNYLARLVFPDKTTEFMVTVDLVAAMAVYNPFDFFLEPRAEQF
                     PFDYEAALHHDLLPYLAREPLTPRWREFVASVDLNPQRTVDFLVALNQRLQREIRYLI
                     RMEPGVQTPEETLQQAAGSCRDTGWLLVQTLRHIGLAARFVSGYLIQLKPDVKSLDGP
                     SGAEHDFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHIPVACTPDPSTAAPVSGSVD
                     ECEVDFRHAMAVTRIFESPRVTKPYTEQQWSEVMAVGRQVDRELDAMDVRLTMGGEPT
                     FVSADDRDGAEWNTSALGPTKRGYATDLVQRLRSRYGAGGFLHCGQGKWYPGEQLPRW
                     ALSVYWRADGQPCWHDPDLFADEREPLKSTNADARRFITRVAQRLRLDAGHVQPGFED
                     VWYYLWRERRLPVNVDPFDARLEDELERIRLRRVFAHKLDHEVGYALPLRRNDDASLA
                     GPRWLTGAWFFRDERMYLIPGDSPMGYRLPLDSLPWVSQGDYPYLHPYDQFAPRGPLP
                     LQADIRAQYAPHAIGGGFAEGGAVALQAGFEGVDGQGERHRAEEPTERPTGSDRFPAR
                     HESAAWVTRTALCVEVRDPQRMNGPQAERDFGGRSGLLYVFMPPLAQVDDYLELLGAV
                     ESTAAELGQKIVLEGYPPPRDPRLKMLQITPDPGVMEVNIHPAHDWDELVDHTEFLYE
                     AAHASRLSTEKFMLDGRHTGTGGGNHFVLGGATPADSPFLRRPDLLASLLGYWHNHPS
                     LSYLFSGLFIGPTSQAPRIDEARNDQLYELEIALAELARSRERNGRDVPPWLVDRTLR
                     NLLIDVAGNTHRAEFCIDKLYSPDSASGRLGLLELRAFEMPPHARMSLTQQLLLRALV
                     ARFWRAPYAAPLTRWGTELHDRFMLPTFVEMDFADVLAEMAQAGYALDPAWFAPHFEF
                     RFPLLGEVVTRGIHLTLRAALEPWHVMGEEGRAGSTVRYVDSSLERVEVKVTGLNESR
                     YAITCNGTALPLQPTGRVGEHVAGVRFRAWQPAASLHPTIGVHAPLTFDIVDRWSERS
                     LGGCRYHVAHPGGRSYDLFPVNAYEAESRRLARFFRIGHTPGRMQVGPADRSREFPFT
                     LDLRR"
     gene            3489..6071
                     /locus_tag="AC16HB_17505"
     CDS             3489..6071
                     /locus_tag="AC16HB_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HIR5608642.1"
                     /translation="MPQASFPFDPASEASHDPAQIALRLALPVRAGHYDELRGSDGSL
                     RAEWRRFFCLLGAGGFDDLDRRFATLVRQIRDDGVTYNVYSDESGSPRPWSLDLLPFI
                     ISAAEWARIEAGVAQRAALLSHIMADVYGAQRLLADGLLPPALVLGSPGYLRPLAGCA
                     PAGDTYLHIVAFDLARGPDGAWWVVSQRTQSPSGIGYALQNRLIKRSLFPEAFREMRV
                     QRLASSYRRLLDTMVRLAPRDDASGVPPRVVLLTPGPYSETYFEHAYLARYLGIPLVE
                     GSDLTVRDEEVFLKTLHGLQRVHAILRRLDDDFCDPLELRPDSTLGVPGLLQAIRARR
                     VVVANAIGSGFLESPALMGFLPPIARRLLGAELLLPSLASWWCGEGAAYEEIRDRLAD
                     KVIKPTYTHTLYRPSFEALMGVGLDDAQRASLRRRIEADPDAYTVQAYLPLSQAPTWQ
                     NRAIVPRSAMVRVFAISDGQGRWHAMPGGLTRVAARDLQVVSMQRGGSSADTWVLTDG
                     VVDDFSMLPVPLRPEDIAGQRRVVTSRAAENLYWMGRYAERAEFSVRLARAIITRLGD
                     DEADATAVLDAMGHLAAQHGLVPEGVPSPASSPQGATIFERVLHAGLTVAHGMTGVEF
                     NLTALTRAAGQIRERLSPEHWRLLVETASGFRDDCARARAGGSYSADEVLGVLARLGV
                     QLAAITGAQTDRMTRDDGWRLLAIGRHVERLGALAQALRVLIEREALAHEEGFELVLE
                     LFDSVITYRSLYQRRQEMPPLIDLLVINTDNPRALAGVARRLLREMQRLPPHAAADLL
                     ARLPAPDTWTPLAELCATDAAGRYATLSTFVARLSAAVDTLSDDIGARFFSHAADRMR
                     LLSV"
     gene            6073..>6598
                     /locus_tag="AC16HB_17510"
     CDS             6073..>6598
                     /locus_tag="AC16HB_17510"
                     /inference="COORDINATES: protein motif:HMM:NF019977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HIR5608643.1"
                     /translation="MSAERLLDVTHDTITRYEERVAVACHVAYLQPRGSERQQVEDFQ
                     LLIEPPPSQRSSGDDVFGNRRICFALYAPHEELHVRSTCRVRLRAAPLPVPEAGVPWG
                     EVASRLRFRAGAPFEPAAEFAYATPFVPLLRELRDYALVSFPAGRTTLQGAIDLMRRI
                     HADFRYQSGATEIST"
BASE COUNT          963 a         2267 c         2277 g         1091 t
ORIGIN      
        1 tcgaacgcga gagcttgcgt ggccatacac gtcgccctga atcacgtcac ccactaccgc
       61 tacgaccggc gggtgggcct gtcgccgcag gtcgtgcgcc tgcgtccggc gccgcacacg
      121 cgcacgccga tcctcgctta ctcgcttcgg gtcgaacctg ccggccactt cgtcaactgg
      181 cagcaggatc ctttttccaa ctacctcgcg cgcctggtct ttccggacaa gacgaccgag
      241 ttcatggtga cggtcgacct cgtcgccgcg atggcggtgt acaacccctt cgatttcttc
      301 ctcgagccgc gcgcggagca gttcccgttc gactacgagg cagcgctgca tcacgacctg
      361 ctgccgtatc tggcgcggga gccgctcaca ccgcgctggc gcgagttcgt cgccagcgtc
      421 gacctgaatc cgcagcgcac ggtggacttc ctggtcgcac tgaatcagcg gttgcagcgc
      481 gagatccgat acctgatccg aatggaaccc ggggtgcaga cgccggagga gactctgcag
      541 caggcggcgg gctcgtgccg cgacaccggc tggctgctgg tgcagacgct gcgccatatc
      601 ggactggcgg cgcgcttcgt gtccggctac ctgatccagt tgaagccgga cgtgaagtcg
      661 ctcgacggtc cgtctggagc ggagcacgac ttcaccgatc tgcacgcgtg gtgcgaggtc
      721 tacctgcccg gcgccggctg gatcggcctg gatccgacgt cggggctgct ggccggcgaa
      781 gggcacatcc cggtcgcatg cacgcccgac ccgagcacgg cggcgccggt ctcgggcagc
      841 gtcgacgagt gcgaggtcga tttccggcac gcgatggcgg tcacgcgaat cttcgaatcg
      901 ccgcgcgtca ccaagccata cacggagcag cagtggtcgg aagtgatggc ggtcggccgt
      961 caggtcgacc gcgagttgga cgcgatggac gtgcgtttga cgatgggcgg cgagccgact
     1021 tttgtctccg ccgacgatcg cgacggcgcg gaatggaaca cgagcgcgct cggcccgacc
     1081 aagcgcggct acgcgaccga tctcgtgcag cggctgcgct cgaggtacgg cgccggtggc
     1141 tttctgcatt gcggccaggg caagtggtat ccgggcgaac aactgccgcg ctgggcgctg
     1201 tcggtgtact ggcgcgcgga cggacagccg tgctggcacg atccggacct gttcgccgac
     1261 gagcgcgagc cactgaagag cacgaacgcc gacgcgcggc gcttcatcac acgggttgcg
     1321 cagcggctgc ggctcgacgc ggggcacgtc cagccggggt tcgaggacgt ctggtactac
     1381 ctgtggcgag agcggcgcct gcccgtgaac gtcgatccgt tcgacgcccg gctggaggac
     1441 gagctggagc gtatccgcct gcggcgcgta tttgcgcaca agctcgatca cgaagtcggc
     1501 tatgccttgc cgctccggcg caatgacgac gcgtcgctcg cgggtccgcg ctggctgaca
     1561 ggcgcatggt tcttccgcga cgaacgcatg tacctgatcc cgggcgattc gccgatgggc
     1621 tatcggctgc cgctggactc tctgccgtgg gtatcgcagg gcgactatcc gtacctgcat
     1681 ccgtacgatc agttcgcgcc gcgcgggccc ctgccgttgc aggcagacat ccgcgcacag
     1741 tacgcgccgc acgcgatcgg cggcggtttt gcggaaggcg gcgcggtcgc attgcaggcc
     1801 ggcttcgagg gagtcgacgg acagggcgag cgccaccgtg ccgaagaacc gaccgagcgg
     1861 ccaacgggat cggatcgatt cccggcgcgg cacgagtcag cggcatgggt cacgcgaacc
     1921 gcgctttgcg tcgaggtacg cgacccgcag cgaatgaacg gcccccaggc agagcgcgac
     1981 ttcggcggcc ggtccgggct gctgtatgtg ttcatgccgc cgctggcgca ggtcgacgat
     2041 tacctcgaat tgctgggcgc cgtcgagtcg actgcagccg agctgggcca gaagatcgtt
     2101 ctcgaaggct atccaccgcc gcgggatccc aggttgaaga tgcttcagat cacgcccgat
     2161 cccggcgtca tggaggtcaa cattcatccc gcgcatgact gggacgaact ggtcgaccac
     2221 accgagttcc tgtacgaggc cgcgcatgcc tcgcgcctgt cgaccgagaa gttcatgctc
     2281 gacggacgcc ataccggcac cggcggtggc aatcacttcg tgctcggtgg cgccaccccg
     2341 gccgacagtc cgttcctgcg ccggcccgat ctgctcgcaa gcctgctcgg ctactggcac
     2401 aaccatccgt cgctttcgta cctgttctcg ggcctgttca tcggcccgac gagccaggcg
     2461 ccgcgcatcg acgaggcgcg caacgatcag ctgtacgaac tcgagatcgc ccttgccgag
     2521 ctcgcccgca gccgcgaacg caacggtcgg gacgtgccgc cctggctggt cgaccgcacg
     2581 ctgcgcaacc ttctgatcga cgtcgccggg aacacgcatc gcgccgagtt ctgcatcgac
     2641 aagctgtatt cgcccgactc tgccagcggc cgcctcgggc tgctggaact gcgcgcgttc
     2701 gagatgccac cgcatgcgcg catgagcctg acgcagcagc ttctgctgcg cgcgctggtg
     2761 gcgcgcttct ggcgcgcgcc ttacgcggcg ccgctgacgc gctggggtac ggaactgcac
     2821 gaccgcttca tgctgccgac cttcgtcgag atggacttcg cggacgtgct ggccgagatg
     2881 gcgcaggccg gctacgcact cgatccggcg tggttcgcgc cgcacttcga gttccgcttt
     2941 ccgctgctcg gcgaggttgt cacgcgcggc atccacctga cactgcgcgc ggcgctcgag
     3001 ccgtggcacg tgatggggga agaaggcagg gcgggcagca ccgtgcgcta cgtcgactcc
     3061 tcgctcgagc gggtcgaggt caaggtcacc gggttgaacg aaagccgcta cgcgatcacc
     3121 tgcaatggca cggcgttgcc gctgcagccg acgggtcgcg tcggggaaca cgtggcgggc
     3181 gtgcgctttc gcgcctggca gccggccgcg tcgctgcatc cgaccatcgg cgtgcatgcg
     3241 ccactgacct tcgacatcgt cgatcgctgg tccgagcgct cgcttggcgg ctgccgctac
     3301 catgtcgccc atcccggcgg ccgcagctac gacttgtttc cggtcaacgc atacgaggcg
     3361 gaaagccgac gcctcgcgcg cttctttcgc atcggccaca cgcccggccg gatgcaggtc
     3421 ggcccagccg accgcagccg cgagtttccg tttacgcttg acctgcgacg ttgaccgacg
     3481 gccgatcttt gccgcaagcc agttttccat tcgaccccgc gagcgaagcc tcgcacgatc
     3541 ccgcgcagat cgcgctgcgg ctggcgctgc cggtccgcgc ggggcattac gacgaactgc
     3601 gtggctccga cgggtcgctg cgcgccgaat ggcgccgttt cttttgtctg ctcggcgccg
     3661 gcggcttcga cgatctcgac cggcgctttg cgacgctcgt gcgccagatt cgcgacgatg
     3721 gcgtcacgta caacgtctac agcgatgaga gcgggtcacc gcggccatgg tcgctcgatc
     3781 tgctgccatt catcatctcg gcggccgagt gggcgcgcat cgaggccggc gttgcgcagc
     3841 gcgcggcgct gctgtcgcac atcatggccg atgtctacgg cgcgcagcgg ttgcttgccg
     3901 acggactgct gccgcccgcg ctcgtgctcg gcagtcccgg ctacctgcgg ccgctcgcgg
     3961 ggtgcgctcc ggccggggat acgtacctgc acatcgtcgc cttcgatctg gcgcgcgggc
     4021 cggacggcgc gtggtgggtc gtttcgcagc gcacgcagtc gccgtccgga atcggctatg
     4081 cgctgcagaa ccgcctgatc aagcgcagcc tgtttccgga ggcattccgc gagatgcgcg
     4141 tgcagcggct ggcatcgagc taccgccgcc tgctcgatac gatggtccgg ctggcgccgc
     4201 gtgacgacgc ctccggagtg ccaccgcgcg tcgtgctgct gacgccggga ccgtacagcg
     4261 agacctactt cgagcacgcg tatctggcgc gctacctcgg cattccgttg gtcgagggca
     4321 gcgacctcac cgtgcgcgac gaggaggtct ttctgaagac gctgcacgga ctgcagcgtg
     4381 tgcatgcgat cctgcgacgc ctggacgacg acttctgcga tccgctcgag ttgcgccccg
     4441 attcgacgct cggcgtcccg gggttgttgc aggcgatccg cgcgcgtcgg gtcgtggttg
     4501 ccaatgccat cggctcgggt ttcctcgaat cacccgcgct gatggggttc ctgccgccga
     4561 tcgcgcgccg cctgctcggc gcggaactgc tgctgccgtc gctggcgtca tggtggtgcg
     4621 gcgagggggc ggcgtacgag gaaatccgcg accggctcgc cgacaaggtc atcaagccga
     4681 cctatacgca tacgctgtat cggccttcgt tcgaggcgct gatgggcgtc ggcctcgacg
     4741 acgcgcagcg cgcgtcactg cgccggcgca tcgaggccga tcccgacgcc tacacggtgc
     4801 aggcctatct gcccctgtca caggcgccga cctggcagaa ccgcgccatc gtgccgcgtt
     4861 cggcgatggt gcgggtgttc gcgatcagcg atggccaggg tcgctggcac gcaatgcccg
     4921 gaggactgac gcgcgtggcg gcgcgcgacc tgcaggtggt gtcgatgcag cgcggtggca
     4981 gcagcgcgga tacctgggtc ctcaccgacg gcgtggtcga cgacttcagc atgctgccgg
     5041 tgccgctgcg ccccgaagac atcgcgggtc aacggcgcgt cgtcaccagt cgcgcagcgg
     5101 aaaatctgta ctggatgggc cgctacgcgg agcgcgccga gttcagcgtg cgcctggcgc
     5161 gcgcgatcat cacgcgactg ggcgacgacg aggcagatgc gacggcggtg ctggacgcga
     5221 tgggacacct cgcggcccag catgggctgg ttccggaggg cgtgccgtcc ccggcgagct
     5281 cgccgcaggg cgcaacgatc ttcgaacgcg tgctgcacgc ggggctgacg gtcgcgcacg
     5341 gaatgaccgg cgtcgaattc aatctgactg cgctgacgcg cgcggccggc cagattcgcg
     5401 agcggctgtc gcccgagcac tggcgcctgc tggtcgagac ggcgagcggc ttccgcgacg
     5461 actgcgcgcg cgcccgcgcc ggcggcagct attcggccga cgaggtgctc ggcgtgctgg
     5521 cgcggctggg agttcagctg gcggcgatca cgggcgcgca gacggatcgc atgacgcgcg
     5581 acgacggctg gcgcctcctc gcgatcgggc ggcacgtcga acgactgggc gcgctggcgc
     5641 aggcgctgcg cgtcctgatc gaacgcgagg cgctggcgca cgaagagggc ttcgaactcg
     5701 tgctggaact gttcgacagc gtgatcacgt accggtcgct gtaccagagg cggcaggaga
     5761 tgccgccgct gatcgacctg ctggtgatca acaccgacaa cccgcgcgcg ctcgccggcg
     5821 tggcgcgccg cctgctgcgc gagatgcagc gcctgccgcc ccacgccgcg gccgacttgc
     5881 tggcgcgcct gccggcgccg gatacatgga cgccgctggc ggagttgtgc gcgaccgacg
     5941 ccgccggccg ctacgcgacg ctttcgacct tcgttgcgcg cctgtccgcc gccgtcgaca
     6001 cgctatcgga cgacatcggc gcgcgcttct tcagccacgc cgccgaccgc atgcgactgc
     6061 tgagcgtctg acatgagcgc cgaacgcctg ctcgacgtca cgcacgacac gatcacgcgt
     6121 tacgaagaac gcgtggcggt agcgtgtcac gtcgcctatc ttcagccccg cgggagcgag
     6181 cggcagcagg tcgaggattt tcagcttctg atcgagccgc cgccgtcgca gcgctcgtcc
     6241 ggcgacgacg tgttcggcaa tcgtcgcatc tgcttcgcgc tgtatgcccc gcacgaggag
     6301 ctgcatgtgc gctcgacctg ccgcgtgcgc ctgcgggccg cgccgctgcc cgtgcccgaa
     6361 gccggcgtgc cttggggcga ggtcgcgtcg cgtctgcgct tccgtgccgg ggcgccgttc
     6421 gagccggcgg ccgaattcgc ctacgcgacg ccgttcgttc cgctgctgcg agagctgcgc
     6481 gactacgcgc tcgtttcgtt tccggccggc cggacgaccc tgcagggcgc gatcgacctg
     6541 atgcgacgca tacacgcgga cttcaggtat cagagcggcg ccaccgaaat cagcacac
//
LOCUS       DBNHBY010000748         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_395002, whole genome shotgun sequence.
ACCESSION   DBNHBY010000748 DBNHBY010000000
VERSION     DBNHBY010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_395002"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16HB_17515"
     CDS             <1..462
                     /locus_tag="AC16HB_17515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5608644.1"
                     /translation="DFELKTFLESGVSLLVATCDAGNAPSITHAGGCRIDESGRMRIY
                     VAAEHAADCLDDVRATGRVATIAVLPHTWECYQVKGNEAHVVALDGADRARVAEYRRQ
                     LFANLARAGVPESQCAGLLPADADAFIGIEFAPTEVYCNTPAGRALGDATS"
     gene            459..1790
                     /locus_tag="AC16HB_17520"
     CDS             459..1790
                     /locus_tag="AC16HB_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5608645.1"
                     /translation="MTRPLAQLRHCLEGPVYPAIATCAPDGTPNVGFLSYVNLVDDEH
                     VALAFRFLDKTARNIAANPRVAVLLTDARTCRQHALDLEYQRTESVGPLYEQMRVRFE
                     AAAALSGMTGVVQPPGVQVYRVLGHRALDRGYVPEPREPAVSAHAVRQLSAWIGACED
                     LSTLLDVTLEGLAEHLHWQHAIVLLADADGTRLYAVASHGYPQSGAGAEVEFGAGPIG
                     VAAAARHPVRVAFAARDRMVNRWIERRGDVDGMERDIPLVGLPDAQSKLAVPILVRNR
                     LLGVLYVESAQASRFLEDDEKTLVALAGHLGTTILLCGQAAEAPPPTRPTARAAAPAG
                     TPLLVRRYEADQSVFLGDDYLIKGVAGAILWKLLTTFTREGRTEFSNRELRLDRSLGL
                     PEIGDNLEARLVLLVRRLRDRSAYIRLERIARGRVRLVVARPLELQSIARA"
     gene            complement(1793..2278)
                     /locus_tag="AC16HB_17525"
     CDS             complement(1793..2278)
                     /locus_tag="AC16HB_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608646.1"
                     /translation="MIRKLHHSAYRCRDSEETRRFYEEFLGMLLAGALEIHETKTGRR
                     TRVLHTFFRLDDGSFLAFFEAPDMPFDFKPQHDYDLHIALEVDAGVLPRVRERAEAAG
                     VEVRGISDHGFIHSIYLRDPNGYVVELAARVPEGVDAMDARLNDARGILERWQKAKQA
                     A"
BASE COUNT          392 a          861 c          870 g          394 t
ORIGIN      
        1 gatttcgaac tgaagacgtt cctcgaaagc ggcgtgtcat tgctcgtcgc gacctgcgat
       61 gccggcaacg ctcccagcat cacgcacgcc ggcggatgcc gcatcgacga aagtgggcgc
      121 atgaggatct atgtcgcggc cgagcacgcg gcggactgcc tcgacgacgt gcgcgcgacc
      181 ggtcgcgtcg cgacgatcgc cgtgctgccg cacacctggg agtgctatca ggtcaagggc
      241 aacgaggcgc acgtcgtggc gctggacggt gccgaccgcg cgcgcgtggc cgagtatcgg
      301 cggcaactct tcgcgaacct cgcgcgcgcg ggcgtgccgg aatctcaatg cgccgggctg
      361 ctgccggccg atgccgatgc gttcatcggc atcgagttcg cgccgaccga ggtctactgc
      421 aacacgccgg ccggtcgcgc gctgggggat gcgacgtcgt gacgcggccg cttgcgcagc
      481 tgcgccattg cctcgaagga ccggtctatc cggcgatcgc gacctgcgcg cccgacggca
      541 cgccgaacgt cggctttctg tcgtacgtga acctggtcga cgacgagcac gtcgcgctgg
      601 ccttccgctt cctcgacaag accgcacgca acatcgccgc caatccgcgc gttgccgtgc
      661 tgctgaccga tgcacgcacc tgtcgtcagc acgcgctcga cctggagtac cagcgcaccg
      721 aatcggtcgg gccgctgtac gagcagatgc gcgtgcgctt cgaggccgcc gcggcgctct
      781 ccggcatgac cggcgtggtg cagccaccgg gcgtgcaggt ctatcgtgtg cttggccatc
      841 gtgcgctcga tcgcggctac gtgccggagc cgcgcgagcc tgccgtgtcc gcgcacgcgg
      901 tgcgccagct gagcgcgtgg atcggcgcgt gcgaagacct gtccacgctg ctcgacgtga
      961 cgctcgaagg gctggccgag catctgcact ggcagcacgc gatcgtgctg ctggccgatg
     1021 cggacggcac ccgcctgtac gcggtcgcga gccatggcta tccgcagtcg ggcgccggcg
     1081 cggaagtgga gttcggcgcg ggcccgatcg gcgtggcggc ggccgcacgc catccggtcc
     1141 gcgttgcatt cgcggcgcgc gaccgcatgg tcaaccgctg gatcgagcgc cgcggcgatg
     1201 tcgacggcat ggagcgcgac atccccctgg tcgggttgcc ggatgcgcag agcaagctcg
     1261 cggtgccgat cctcgtgcgc aatcgcctgc tcggcgtgct gtacgtcgaa agcgcgcagg
     1321 cgagccgctt cctcgaggac gacgagaaga cgctggtcgc cttggccggc cacctaggca
     1381 ccacgatcct gctttgcggc caggccgccg aagcaccgcc gccgacccgc cccacggcgc
     1441 gcgcggcagc gcctgcgggc acgccgctgc tggtgcgacg ctacgaggcc gaccagagcg
     1501 tcttcctcgg cgacgactac ctgatcaagg gcgtggccgg cgcgatcctg tggaagctgc
     1561 tgacgacctt cacgcgggaa ggacgcaccg agttcagcaa tcgcgagctg cgcctcgacc
     1621 gctcgctcgg cctgcccgaa atcggcgaca acctcgaagc gcggctggtg ctgctggtgc
     1681 ggcggctgcg ggaccgcagc gcatacatcc gcctcgagcg aatcgcgcgc ggccgcgtgc
     1741 gcctggtggt cgcgcggccg ctcgaactgc agtcgatcgc gcgcgcatag cgctacgcgg
     1801 cctgcttcgc cttctgccag cgctcaagga tgccgcgcgc atcgttgagc cgcgcatcca
     1861 tcgcgtcgac accttccggc acgcgcgcgg cgagctcgac cacgtagccg ttcggatcgc
     1921 gcaggtagat ggagtggatg aagccgtggt cgctgatgcc gcgcacttcg acgcccgcgg
     1981 cctcggcgcg ctcgcgtacg cgcggcagca caccggcatc gacctccagc gcaatgtgca
     2041 ggtcgtaatc gtgctggggt ttgaagtcga acggcatgtc gggcgcctcg aagaacgcca
     2101 ggaacgagcc gtcgtcgaga cggaagaacg tatgcagcac gcgcgtgcga cggccggtct
     2161 tggtctcgtg aatctcgagt gctccggcga gcagcatgcc gagaaactcc tcgtagaagc
     2221 ggcgtgtttc ctccgaatcg cggcaacggt aggcgctgtg gtgcagcttg cggatcatcg
     2281 ggtgtcctct gcgtgtcggg tgaagagtgg atggtcgccg gacgcaggac gtgcggttcg
     2341 taaatgttga ttcatgaaac cctaaaagtc ccttaacggg cacgattcgg cggctgcagg
     2401 caaaatacgg ctcccgccat ggatgtcccc gaacacaaga acacgccgtc gaccgaacgg
     2461 cgcgaccgcc tccttgccct gtaccgcacg atggcgcgca tccgcgcctt cgaggac
//
LOCUS       DBNHBY010000749         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_328025, whole genome shotgun sequence.
ACCESSION   DBNHBY010000749 DBNHBY010000000
VERSION     DBNHBY010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_328025"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16HB_17530"
     CDS             complement(<1..534)
                     /locus_tag="AC16HB_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608647.1"
                     /translation="MTTSALRAWSRSMLPLMLTAIVTLLGCSTVDTGRADAERTQATP
                     ASAPVQQGTAIRDFSAGLRCMDTLLLDYGTHDISVMVEDLVDQAQRSNAGARDLLIAV
                     VSDMTQRSRAIRVVASGKEWGNTLSQLSRVQPAAAVVPQYALRGSLGRRNGDGAAGSD
                     AQARSALELDLTLLTTSD"
     gene            862..1428
                     /locus_tag="AC16HB_17535"
     CDS             862..1428
                     /locus_tag="AC16HB_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR5608648.1"
                     /translation="MNLPPISFGNNIYTGAGNDNVHISKAPGLLGALGLYEVNINGNV
                     QYMTKQQLENTNFHLGAGNDTLVVDSNVTASIHADGGSGNDVMIGGAGNDVLKGGSGD
                     DVIAGRGGNDRIDGGRGNDWLFGGDGNDVITGGPGRDHLYGGNGNDALFGGRGNDVLD
                     GGRGNDLLVGGPGFDWLNGGPGFDIKLP"
BASE COUNT          277 a          537 c          524 g          240 t
ORIGIN      
        1 gtcactggtc gtcagcagcg tcaggtcgag ctcgagtgcc gatcttgcct gcgcatccga
       61 gccggcggcg ccgtcgccgt tgcgccggcc gagcgatccg cgcagcgcgt actgcggcac
      121 gactgctgcc gcaggctgaa cccgggacag ctgactcaac gtgttgcccc actccttgcc
      181 cgacgccacg acccggatcg cgcggctgcg ctgcgtcatg tcggaaacga ccgcgatcaa
      241 cagatcccgc gcgcccgcgt tgctgcgctg cgcctgatcg acgaggtctt cgaccatcac
      301 cgagatgtcg tgcgtgccgt agtcgagcag gagcgtgtcc atgcagcgca gcccggcgga
      361 gaaatcgcgt atagcagttc cttgctgcac cggcgcactc gctggcgtcg cctgcgtccg
      421 ctcggcatca gcgcggccag tgtcgaccgt gctgcaaccc agcagcgtca cgatcgccgt
      481 gagcatcagc ggcagcatgc tccgcgacca ggcgcgcagc gcagacgtcg tcatgacgat
      541 cctccttcgg ccttgcggcg cggccaacgc cagtggcact gcagactcat gcgcgacggc
      601 gagtttccag cattgatgcg cgaagtccgg ctcgggtacg cgttcgcgag cccgtccttc
      661 agttgcggtg ctcggacgat gggttcaaac aagcgtgcgg gaatcgaggc tggtttcgcc
      721 ggccaccagt gggagcggcc ctagctgggg cgcgtcccgg ctattcgttc agcgcggcag
      781 acgttcaaat gcgactcagc gatgcggaac cctgaactgt gtcgcctcac cccttaacca
      841 ctcacgcaac ggagcatcac catgaacctc cctcccatct cgttcggcaa caacatctac
      901 accggtgccg gcaacgacaa cgtgcacatc tccaaggcgc ccggactgct cggcgcgctg
      961 ggcctgtacg aagtgaacat caatggcaac gtccagtaca tgaccaaaca gcagctggag
     1021 aacaccaact tccatctcgg cgcgggcaac gacacgctcg tggtcgacag caacgtcacc
     1081 gccagtatcc atgccgacgg cgggtcgggc aatgacgtaa tgatcggcgg cgccggcaac
     1141 gacgtcctga agggcggcag cggcgacgac gtgatcgcgg gccgcggcgg caacgaccgc
     1201 atcgacggcg ggcgcggcaa cgactggctg ttcggcggcg acggcaacga cgtcatcacc
     1261 ggcgggcccg gccgcgacca cctctacggc ggcaacggca acgacgcgct gttcggcggg
     1321 cgcggcaacg acgtgctcga cggcggccgc ggcaacgacc tgctggtcgg cgggcccggg
     1381 ttcgactggt tgaacggcgg gccgggcttc gacatcaagc tgccgtaatc cggtcgcggc
     1441 gggtcacccg cagcaagcgc ccgaccgcgc tcgtttcggc acattgccgg gcggcccggt
     1501 cgggcgtttg tctttaccag cggatcgaag cggacagctc cggtgcggag cgagaagggt
     1561 gggaaaagcg gattggga
//
LOCUS       DBNHBY010000750         5004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_462617, whole genome shotgun sequence.
ACCESSION   DBNHBY010000750 DBNHBY010000000
VERSION     DBNHBY010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_462617"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16HB_17540"
     CDS             complement(<1..223)
                     /locus_tag="AC16HB_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR5608649.1"
                     /translation="MSDYLADDQPVDAHAFTRRACDPRESVVVEACAGSGKTWLLVGR
                     IVRLLLAGAAPGEILAITFTRRAAQEMRTR"
     gene            complement(220..2955)
                     /locus_tag="AC16HB_17545"
     CDS             complement(220..2955)
                     /locus_tag="AC16HB_17545"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR5608650.1"
                     /translation="MLERQSIAADAGFWPAAALAVTEFAARCGSAQDRLHGLSWIVPT
                     GAHAAPARAGLRNALRGAAFIPPRIAPLASWLGQPMQASVAARAELFSALRGNRWVRE
                     AFGDQPAALWSLARGIAQLCEELTWAAIDGEAAFTGRVEASLARHFHRRAAIALQPQA
                     QLVLQLWRARRADERAARALRELDARANAASTPLVYTSADELQRWEAAFLERYAQRAP
                     VLLLAADGCAALRARPLLAAAWPELCDGERQMPLAARAATVGGASMPLTIVRTNSLEE
                     EATAVARQVLDWLRDGVGPIALVALDRLTARRARALLDRAQVNVRDETGWKLSTTSAA
                     AAVMRWFDLVADDLYWRDLLDFLKSPFTLVDRAGKAADAALIERAIRAGSTVQGARAI
                     RRAAALQAAREDMDGRGADELLALIDAQAQVARRAGSTLVEHARALGAALSALGLRAG
                     LEADPVGRDVLRELDALEAELAAVAGRASRADFRALLAERFEEIAYVDRQVESPVVMV
                     SLAASHLRAFGAALLIGADARHLPGDRNEALFMSNAVRAELGLATAEAELRRQSAQLA
                     ALLATVPRLAATWRVQHGDEPNPLSPLLERLQFVAARALGDDLLRDAPGEESAVDAVA
                     QSRPAPAAPTLLPASISASSAQSLVQCAYQFYARWLLDLAELDDVIELPDKRDFGEAL
                     HEVLRRFHHEWGGADFATLEAPALAESLRAHARAVFEPLLERMPALLAFQRRFDGLVD
                     GYIGWLRQCARDGWRWQAAEQTHAQRWPLPDGREVELVGRIDRIDAHGDGRVRVLDYK
                     ARARDVLARGLREAGEDVQLPFYGLLLARPQASAAYVSLDRARDNDGGVHEVAPPQPF
                     AALVERVAQRLRDDIARVAAGAPLPALGVEAVCEYCEMRGLCRRDYWSDAQREAG"
     gene            3135..3461
                     /gene="trxA"
                     /locus_tag="AC16HB_17550"
     CDS             3135..3461
                     /gene="trxA"
                     /locus_tag="AC16HB_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR5608651.1"
                     /translation="MTQEIKYISDASFESDVLKADRPVLVDYWAEWCGPCRMIAPILD
                     EVAGDYKGKLTVAKLNVDENQAVPAKYGIRGIPTLMLFKNGAVVGTKVGALSKSQLTA
                     FIDSNI"
     gene            3551..4813
                     /gene="rho"
                     /locus_tag="AC16HB_17555"
     CDS             3551..4813
                     /gene="rho"
                     /locus_tag="AC16HB_17555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5608652.1"
                     /translation="MQLSELKAQHVSQLLDMAGTLEIDNANRMRKQELMFAILKKRAK
                     GGEQIFGDGTLEVLPDGFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     TPKDGERYFALVKVDKVNGQPPESTKHRILFENLTPLFPDEPLKLEREIKAEENITGR
                     LIDIIAPIGKGQRGLLVSSPKSGKTVLMQHVAHAIAANHPDVVLIVLLIDERPEEVTE
                     MQRTVRGEVVASTFDEPAARHVQVAEMVIEKAKRLAESKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLERRLAEKRVYPAINVNRSGTRKEELLLKPDILQKVWILRKLLYGMDE
                     IEAMEFLLDKMRQTKSNAEFFEMMRRGG"
     gene            4905..>5004
                     /locus_tag="AC16HB_17560"
     CDS             4905..>5004
                     /locus_tag="AC16HB_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR5608653.1"
                     /translation="MKPGIHPEYREVVFIDTSNDFKFITRSTVQTKE"
BASE COUNT          845 a         1778 c         1687 g          694 t
ORIGIN      
        1 gccgcgtgcg catctcctgc gcggcgcggc gggtgaaggt gatcgcaagg atctcgcccg
       61 gcgcggcgcc cgcgagcaac agccgcacga tgcggccgac cagcagccac gtcttgccgc
      121 tgccggcaca tgcctcaacc accacgctct cgcgagggtc gcaggcgcga cgcgtgaatg
      181 cgtgcgcgtc gactggctgg tcgtcggcga ggtagtcgct catcccgcct cgcgctgcgc
      241 gtccgaccag tagtcgcggc ggcacagccc gcgcatctcg cagtattcgc acaccgcctc
      301 gacgccgagc gcgggcaatg gcgcaccggc cgccacgcgc gcgatgtcgt cgcgcagtcg
      361 ttgcgcgacg cgctcgacca gcgcagcaaa cggctgcggt ggagccacct cgtgcacgcc
      421 gccgtcgttg tccctggcgc gatcgaggct gacataggcg gcgctcgcct gcggccgtgc
      481 aagcagcagg ccgtagaaag gcagttgcac atcctccccg gcctccctca gcccgcgcgc
      541 gaggacgtca cgcgcgcgcg ccttgtagtc gagcacgcgg acgcggccgt cgccatgcgc
      601 gtcgatgcga tcgatgcggc ccacgagctc gacctcacgg ccgtcgggca gtggccatcg
      661 ctgcgcatgc gtctgctcgg ccgcctgcca gcgccagccg tcgcgcgcgc actggcgcag
      721 ccacccgatg tagccgtcga cgaggccgtc gaagcgccgc tgaaatgcca gcagcgccgg
      781 catgcgttcg agcagcggct cgaacaccgc gcgcgcgtgc gcgcgcaggc tttccgcgag
      841 cgccggcgct tcgagcgtgg cgaagtccgc gccgccccac tcgtggtgga aacggcgcag
      901 cacctcgtgc agcgcctcgc cgaagtcgcg cttgtccggc agttcgatca cgtcgtcgag
      961 ctcggccaga tccagcagcc agcgggcgta gaactggtac gcgcactgaa ccaggctctg
     1021 cgcgctgctg gcgctgatgg acgccggcag caacgtcggc gcggccggtg ccgggcgcga
     1081 ctgcgccacc gcatcgaccg cggattcctc tcccggggca tcgcgcaaaa ggtcatcgcc
     1141 gagcgcgcgg gcggcgacga actgcagccg ttcgagcagc ggcgacagcg ggttcggctc
     1201 gtcaccgtgc tggacgcgcc acgtcgccgc caggcgcggc acggtggcga gcagcgcggc
     1261 aagctgcgcc gattgtcggc gcaactccgc ctccgccgtc gccagcccaa gttcagcgcg
     1321 taccgcgttc gacatgaaca gcgcctcatt gcgatcgcct ggcaggtgcc gcgcatcggc
     1381 gccgatcagc agcgccgcgc cgaaggcgcg caggtgcgac gcggccagcg acaccatcac
     1441 gaccggcgac tcgacctgcc ggtcgacgta cgcgatctcc tcgaaccgct ccgccagcag
     1501 cgcgcgaaag tccgcccgcg acgcgcgacc cgcgacggcc gccagttcgg cttcgagcgc
     1561 gtcgagttcg cgcagcacgt cgcgacccac cgggtcggct tcgagccccg cacggaggcc
     1621 gagcgccgat agcgcggcgc cgagcgcgcg cgcgtgctcg accagcgtgc tgccggcgcg
     1681 ccgcgcgacc tgcgcctgcg cgtcgatcag cgccagcagt tcgtcggcgc cgcgcccatc
     1741 catgtcttcg cgcgccgcct ggagcgcggc cgcgcgccgg atcgcgcgcg cgccctgcac
     1801 cgtgctgccg gcgcgaatcg cacgctcgat cagcgccgcg tcggcggcct tgccggcgcg
     1861 gtcgaccagc gtgaacggcg acttcagaaa gtcgagcagg tcgcgccagt aaaggtcgtc
     1921 ggcgacgaga tcgaaccaac gcatcaccgc ggcggcggcg ctggtcgtgg agagcttcca
     1981 gccggtttcg tcgcgcacgt tcacttgtgc gcgatcgagc agggcccgcg cgcgccgcgc
     2041 cgtcaaccga tcgagcgcga cgagcgcgat cggaccgaca ccgtcgcgca accagtcgag
     2101 cacctgccgc gccactgcgg tcgcttcctc ctccagcgag ttcgtgcgga cgatggtcag
     2161 cggcatcgaa gcgccgccga ccgtggccgc gcgcgccgcg agcggcatct gccgttcgcc
     2221 atcgcacagc tccggccatg cggccgcgag cagcggtcgc gcgcgcagcg ctgcgcaccc
     2281 atcggcggcc agcagcagca ccggtgcgcg ctgtgcgtag cgctcgagaa atgccgcttc
     2341 ccagcgttgc agctcgtccg cggacgtgta gacgagcggc gtcgatgcgg cgttggcacg
     2401 cgcgtcgagt tcgcgcagcg cgcgcgcggc gcgctcgtcg gcacgccgcg cacgccataa
     2461 ctgcagcacc agctgcgcct ggggttgcag cgcgatcgcg gcacggcgat ggaaatggcg
     2521 cgccaggctt gcctcgaccc ggccggtgaa cgctgcttcg ccgtcgatcg cggcccacgt
     2581 cagttcctcg cacagttgtg cgatcccgcg cgccagcgac cagagcgccg ccggctggtc
     2641 gccgaaggct tcgcgcaccc agcgattgcc gcgcagcgcc gagaacaact cggcccgcgc
     2701 cgccacgctt gcctgcatcg gctgcccgag ccagctcgcg agcggcgcga tgcgcggcgg
     2761 aatgaatgcc gcgcctcgaa gcgcgttgcg caggcccgcc cgcgccggcg ctgcgtgcgc
     2821 accggtcgga acgatccagc tcagcccgtg caggcggtct tgcgcagatc cgcagcgcgc
     2881 ggcgaattcc gtcaccgcga gcgcggccgc gggccagaag ccggcgtccg cggcgatcga
     2941 ctggcgctcg agcatggccc gattgtatga accccatcgc cgggacacgc aaagcgccga
     3001 tgcagtgcta acatgcgctc gtcccaagct ggccggtccg gaattccggg ttgcgcccag
     3061 gcttgtccga atcgcccaag cccccatctc ttccgtgttt ccgtgcgcca cggtcggcgc
     3121 cgagaggatc cgcaatgact caggaaatca agtacatcag cgacgcgagc ttcgaatccg
     3181 acgttctgaa agcggaccgg cccgtgctgg tcgattactg ggccgaatgg tgcggcccgt
     3241 gccgcatgat cgcgccgatc ctcgacgagg tcgcgggcga ctacaagggc aagttgacgg
     3301 tggcgaagct gaacgtggac gagaaccagg cggtgccggc gaagtacggc attcgcggca
     3361 ttccgacgct gatgctgttc aagaacggcg cggtggtcgg caccaaggtc ggcgcgcttt
     3421 cgaagtcgca gctgactgct ttcatcgata gcaacatata aaggacgtcg gcgcgcggcg
     3481 ccgactcctt gaccccccgc gggcgcctcg ccaggtcaac ggcgccccac cactctccct
     3541 tccgataccc atgcaactct ccgaactcaa agcccagcac gtgtcgcaac tgctcgacat
     3601 ggcaggcacg ctcgaaatcg acaatgccaa ccggatgcgc aaacaggaat tgatgtttgc
     3661 gatcctgaag aagcgcgcca agggcggcga gcagatcttc ggcgacggca cgctcgaggt
     3721 gctgccggac ggcttcggct tcctgcgctc gccggaaacg tcctacctgg cgtcgaccga
     3781 cgacatctac atttcgccga gccagatccg ccgcttcaac ctgcacaccg gcgactcgat
     3841 cgagggcgag gtgcgcaccc ccaaggacgg cgagcgctac ttcgcgctgg tcaaggtcga
     3901 caaggtcaac ggccagccgc cggagtcgac caagcaccgc atcctgttcg agaacctgac
     3961 gccgctgttc ccggacgagc cgctgaaact cgagcgcgag atcaaggcgg aggagaacat
     4021 caccgggcgg ctgatcgaca tcatcgcgcc gatcggcaag ggccagcgcg gactgctggt
     4081 gtcgtcgccg aaatccggca agaccgtgct catgcagcac gtcgcgcatg cgattgccgc
     4141 caatcacccg gacgtggtgc tgatcgtgct gctgatcgac gagcggcccg aggaggtcac
     4201 ggagatgcag cgcacggtgc gcggcgaagt ggtcgcgtcg accttcgacg aaccggctgc
     4261 gcggcacgtg caggtggccg aaatggtgat cgagaaggcc aagcggctgg cggagagcaa
     4321 gaaggacgtg gtgatcctgc tcgattcgat cacgcggctg gcacgcgcct acaacacggt
     4381 ggtgccggcg tcgggcaagg tgctcaccgg cggcgtggac gccaatgcgc tgcaacggcc
     4441 gaagcgcttt ttcggcgccg cacgcaacat cgaggaaggc ggttcgctga cgatcatcgc
     4501 gaccgcgctg atcgacaccg gctcgcgcat ggacgacgtg atctacgagg aattcaaggg
     4561 caccggcaac atggagatcc acctcgagcg ccggctggcc gagaagcgcg tctaccccgc
     4621 gatcaacgtg aaccgctcgg gcacgcgcaa ggaagagctg ctgctgaagc ccgacatcct
     4681 gcagaaggtc tggatcctgc gaaagctgct ctacggcatg gacgagatcg aggcgatgga
     4741 attcctgctc gacaagatgc ggcagacgaa gagcaacgcg gagttcttcg agatgatgcg
     4801 gcgaggcggc tgacgccgcc cggattgctt atacttcgct tcttcccgga aactggtacg
     4861 tggcctgagc gcgctcagac tggcggccgc aacgaaagca acccatgaag cccggcatcc
     4921 accccgaata ccgcgaagtc gtgttcatcg acacgtcgaa cgacttcaag ttcatcaccc
     4981 gctcgaccgt gcagacgaag gaga
//
LOCUS       DBNHBY010000751         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_328115, whole genome shotgun sequence.
ACCESSION   DBNHBY010000751 DBNHBY010000000
VERSION     DBNHBY010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_328115"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="AC16HB_17565"
     CDS             complement(<1..1181)
                     /locus_tag="AC16HB_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608654.1"
                     /translation="MLERLIAWSARNPFLIALGTLAIVFAGVLAIARTPLDALPDLSD
                     VQVIVYTEYPGQAPQVVEDQVTYPLSTAMLSVPKSKVVRGFSFFGVSFVYVIFEDGTD
                     IYWARSRVLEYLNFAASRLPQGVSAQIGPDATGVGWVYQYVVQAKNRTLAELRTLQDW
                     YLRYQVTKAQGVAEVASVGGFVQQYQVIVDPARLRAYGIPLMTVAQVIRDSNRDVGGR
                     VVELAETEYMVRGRGYLRSAADIEDLVVRAEKGTAVRVRDVARVELGPDERRGLTELN
                     GEGEVVSGIAVARFGENALRVIENVKQRIEELKPGLPSGVTIRAVYDRSDLIKRAIET
                     LKSTLLEESVIVALVCVLFLLHVRSALVAIVMLPIGVLIAFICMHALGINSNIMSLGG
                     IA"
     gene            complement(1193..>1924)
                     /locus_tag="AC16HB_17570"
     CDS             complement(1193..>1924)
                     /locus_tag="AC16HB_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HIR5608655.1"
                     /translation="LSSVWVIADVFEQDLGRVRVGQMAQLRFDAYPGEAFKGRVTYVY
                     PTLRAEMRTAQVRIELPNPGGKLKPAMYAQVELPSAGAAAVVVPNSAVIDSGRRRVVL
                     VDLGNGRFEPREVKLGARGDEYAAVLDGVKEGESVVVAANFLIDAESNLKAALGSLTA
                     PAPTAGKVAHNATGTLLDVDAGSGALLIAHDPVPSLNWPKMQMEFVPANDAVAKAVKP
                     GSPITFEFVERKPGEWVVTKIEPRR"
BASE COUNT          315 a          698 c          603 g          308 t
ORIGIN      
        1 gcgatcccgc ccaggctcat gatgttcgag ttgatgccca gcgcgtgcat gcagatgaac
       61 gcgatcagca cgccgatcgg cagcatgacg atcgccacca aggcgctcct cacgtgcagc
      121 aggaacagca cgcataccag cgccacgatc accgactcct cgagcagcgt cgatttcagc
      181 gtttcgatcg cgcgcttgat caggtccgag cggtcgtaca cggcccggat cgtcacgccg
      241 gacggcagac cgggcttcag ttcctcgatg cgctgcttga cgttttcgat cacgcgcagc
      301 gcgttctcgc cgaagcgcgc caccgcgatg cccgacacca cctcgccctc gccgttgagt
      361 tccgtcaagc cgcggcgctc gtccggaccg agttcgacac gcgcgacatc gcgcacgcgc
      421 acggcggtgc ccttctccgc acgaaccacc aggtcttcga tgtcagccgc gctgcgcagg
      481 tagccgcgcc cgcgcaccat gtattcggtc tcggcgagtt ccaccacgcg cccgccgaca
      541 tctcggttcg agtcgcggat cacctgcgcg accgtcatca gcgggatgcc gtacgcgcgc
      601 aacctggccg gatcgacgat cacctggtac tgctgcacga agccaccgac cgacgcgacc
      661 tcggccacgc cctgcgcctt cgtgacctga tagcgcaggt accagtcctg cagcgttcgc
      721 agttccgcca gcgtgcggtt cttcgcctgc accacgtact ggtagaccca gccgacgccg
      781 gtcgcgtctg gcccgatctg cgcgctcacg ccttgaggaa gccgcgaggc ggcgaagttc
      841 aggtactcca gcacgcgcga gcgcgcccag tagatgtcgg tgccatcctc gaagatcacg
      901 tacacgaacg acacgccgaa gaacgagaac ccgcgcacga ccttcgactt cggcaccgac
      961 agcatcgccg tcgacagcgg gtaggtgacc tgatcctcga ccacctgcgg cgcctgcccc
     1021 gggtactcgg tgtagacgat cacctgcacg tcggacagat ccggcagcgc gtcgagtggc
     1081 gtgcgcgcga tcgccagcac gccggcgaac acgatcgcga gcgtgccgag cgcgatcagg
     1141 aacggattgc gcgcggacca ggcgatgagt ctttccagca tcgcgggctc cgtcagcgcc
     1201 tgggctcgat cttcgtcacc acccattcgc ccggtttgcg ctcgacgaat tcgaacgtga
     1261 tcggcgagcc gggtttcacg gccttggcga ccgcgtcgtt ggccggcacg aattccatct
     1321 gcatcttcgg ccagttcagc gacggcaccg ggtcgtgtgc gatcagcagc gcgccgctgc
     1381 cggcatcgac gtcgagcagc gtgccggtcg cgttgtgcgc gaccttgccg gcggtcggcg
     1441 cgggcgcagt cagcgagccg agcgcggcct tcaggttgct ctcggcgtcg atcaggaagt
     1501 tggcggcgac gaccacggat tcgccttcct tcacgccgtc gaggaccgcg gcatactcgt
     1561 cgccgcgcgc gccgagcttg acctcgcgcg gttcgaagcg gccgttgccg agatcgacca
     1621 gcaccacgcg ccggcggccg ctgtcgatca ccgccgagtt cggcaccacc acggcggccg
     1681 cgccggcgct cggcagctcg acctgcgcgt acatcgcggg tttcagtttc ccgcccgggt
     1741 tgggcagctc gatgcggacc tgcgcggtgc gcatctccgc cctcagcgtc ggatagacgt
     1801 aagtgacacg gcccttgaac gcctcgcccg ggtaggcgtc gaagcgcaat tgcgccatct
     1861 gaccgacgcg cacgcggccg aggtcctgct cgaacacatc ggcgatcacc cacacgctcg
     1921 acag
//
LOCUS       DBNHBY010000752         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1884444, whole genome shotgun sequence.
ACCESSION   DBNHBY010000752 DBNHBY010000000
VERSION     DBNHBY010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1884444"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..622)
                     /locus_tag="AC16HB_17575"
     CDS             complement(176..622)
                     /locus_tag="AC16HB_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5608656.1"
                     /translation="MRLQTNTSLALFSVLEFARDPSRHIPASEIAEKYGVSAHHLAKV
                     LAELARAGLLESVRGVGGGYRFATNSQRVTMMDVIQLFEDLSPALTARNEGRDAAAVE
                     RALAAVLSEIDELTRATFSSITLATMLRLVERQQTVPTPSEAGQPA"
     gene            complement(630..1112)
                     /locus_tag="AC16HB_17580"
     CDS             complement(630..1112)
                     /locus_tag="AC16HB_17580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5608657.1"
                     /translation="MARTASKTPAAAADEIVVRPVRRGDLEQVIAIDAQVTGLEKQAY
                     WRGVHRRYGTAGEQQRQFLVAVAGKRVIGFVIGEVRDWEFGSPPCGWVFAISVDPQAR
                     QTGVGTRLLQALSAAFRRAGVRKMRTMLARDNTLILSFFRSHGMTAGSLIPLEMALEG
                     "
     gene            1306..1956
                     /locus_tag="AC16HB_17585"
     CDS             1306..1956
                     /locus_tag="AC16HB_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5608658.1"
                     /translation="MTRWAMIADLRRCVGCQTCTAACKVTNATPPGVQWRRVLDMEFG
                     TYPSVQRVFVPVSCQHCDEPPCMDVCPTTATRKRADGVVTIDYDLCIGCAYCAVACPY
                     QQRYKTNRATFAYGEATEAEAQRFDEARLAVATKCTFCVDRIDAGLAHGLKPGVDPEA
                     TPACVNSCITQALAFGDLDDPDSNVSKLLAENQHFRMHEELGTGPGMYYLWDKETA"
     gene            1967..2809
                     /locus_tag="AC16HB_17590"
     CDS             1967..2809
                     /locus_tag="AC16HB_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608659.1"
                     /translation="MTYGPRPWQQTQWDWRAAGNFMFGGAGSGLVVFTALSGVGGAEL
                     TVLLLVGLGLIGLGLSCVWLELGRPLRAINVFFNPRTSWMSREAIAATLLMPAGLAAA
                     FVAPALVWVAAALALAFVYCQSRMLQAAKGIAAWREPRLVPLLLATGLTEGAGLFWLG
                     AAWHGRGTLALLVVFGALVIVRMLAWRAWRQRLAAHAAPRALTAIDAAGKTLLVAGTL
                     VPLAAIVVAGVGQLGAPVTAIAAAGGLLAAVAGAWFKFTLVTRAGFNQGFALTHLPIR
                     GARR"
     gene            2891..3355
                     /gene="hcrC"
                     /locus_tag="AC16HB_17595"
     CDS             2891..3355
                     /gene="hcrC"
                     /locus_tag="AC16HB_17595"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HIR5608660.1"
                     /translation="MSKRLLSLSVNGRAREHAVADHALLVDYLRETLGLTGTKQGCDG
                     GECGACTVLIDGEPRLSCITLAATCEGAHVETIESLAASGRMSRLQQAFHEKLGTQCG
                     FCTPGMIMAAEALLRRNPRPSEAEIREALAGNLCRCTGYVKIIESVQAAAGQ"
BASE COUNT          524 a         1195 c         1259 g          542 t
ORIGIN      
        1 aatcccctgg cagcggcgag gggtgcacga tgatccgggc cagtcgaggc cgtcaatatg
       61 tcgtgcgcac ttccgggagt ggacgacacc ggcgggtcgg cattcgaccc tgcgccggcg
      121 ccacatggtc ggcgcgacgt ccaagctgcg cggctgacgc cgatccggtg ccggctcagg
      181 ccggctgtcc tgcttcgctc ggggttggga ccgtctgctg ccgctccacc agccgcagca
      241 tcgtcgcgag cgtgatcgaa ctgaacgtcg cgcgcgtcag ctcgtcgatc tccgacagca
      301 ccgccgcgag cgcccgctcg accgcggcgg cgtcgcgtcc ctcgttgcgc gcggtgagcg
      361 ccgggctcag gtcctcgaac agctggatca cgtccatcat cgtcacgcgc tgggaattgg
      421 tggcgaagcg gtagccgccg cccacgccgc gcaccgattc gagcaggccg gcgcgcgcca
      481 gttctgccag caccttggcg aggtggtgcg ccgacacgcc gtacttctcg gcgatctcgg
      541 aggccggaat gtggcgcgag gggtcgcgcg cgaactcgag cacgctgaag agcgccagcg
      601 atgtgttggt ctgcagtcgc atcgcgcggt cagccctcca gcgccatctc gagcgggatc
      661 agcgagccgg ccgtcattcc gtggctgcgg aagaacgaca ggatcagcgt gttgtcgcgt
      721 gccagcatcg tgcgcatctt gcgcacaccg gcgcggcgga aagcggcact cagcgcctgc
      781 agcagccgtg tgccgacgcc ggtctggcgc gcctgcggat cgacgctgat cgcaaacacc
      841 cagccgcacg gcggcgagcc gaactcccag tcgcgcactt cgccgatcac gaagccgatc
      901 acgcgcttgc cggccaccgc caccaggaac tgccgctgct gctcgccggc cgtgccgtaa
      961 cggcggtgca cgccgcgcca gtaagcctgc ttctccagcc cggtcacctg agcgtcgatc
     1021 gcgatcacct gttccaggtc gccgcgccgc acgggccgga cgacgatctc gtccgccgcc
     1081 gcggccgggg tcttgctggc agttctcgcc acggtcgtcc gaaaaggatg aactagatca
     1141 aatgtaaacc ggcatttcgg taccagaatg attttaatca ggcggattgt ccagcgcgac
     1201 ggccgccggc acaggagacg aagagcttga aactcccact gcaagcgggc gccggcaggg
     1261 gcgcgacacc ggcgagccgg tcctgaaccg aagggtgtca gcgcgatgac ccgctgggcg
     1321 atgattgccg acctgcgtcg ctgcgtcggc tgccagacct gcaccgctgc gtgcaaggtc
     1381 accaacgcca cgccgccggg cgtgcagtgg cggcgtgtgc tcgacatgga gttcggcacc
     1441 tacccgagcg tgcagcgtgt gttcgtgccg gtgagctgcc agcactgcga cgagccaccc
     1501 tgcatggacg tgtgccccac gaccgcgacc aggaagcgcg ccgacggcgt ggtcacgatc
     1561 gactacgacc tttgcatcgg ctgcgcttac tgcgcggtcg cctgccccta ccagcagcgc
     1621 tacaagacga accgggccac cttcgcgtac ggcgaggcga ccgaggccga ggcgcagcgc
     1681 ttcgacgaag ccaggctggc ggtcgcgacc aaatgcacct tctgcgtgga tcgcatcgac
     1741 gcgggactcg cgcacgggct gaaacccggc gtcgatccgg aggcgacgcc ggcatgcgtg
     1801 aactcgtgca tcacgcaggc gcttgcgttc ggcgacctcg acgacccgga cagcaacgtt
     1861 tcgaagctgc tggcggagaa ccagcacttc cgcatgcacg aggagctcgg caccgggccg
     1921 gggatgtatt acctgtggga caaggaaacc gcatgaaggt ggctgcatga cctacggccc
     1981 gcgcccctgg cagcagacgc agtgggactg gcgtgccgcc ggcaacttca tgttcggcgg
     2041 cgcgggcagc ggactcgtcg tgttcaccgc gctgtcgggg gttggcggcg cggagctcac
     2101 ggtactgctt ctggtcgggc tcggcctgat cggtctcggg ctctcgtgcg tatggctgga
     2161 actcggccgc ccgctgcggg cgatcaacgt cttcttcaat ccgcgcacgt cttggatgtc
     2221 gcgcgaggcg atcgcggcca cgctgctgat gccggccggg ctggcggcgg cgttcgttgc
     2281 gcccgcgctg gtctgggtcg ccgccgcgct ggcgctcgcg ttcgtctact gtcaaagccg
     2341 catgttgcag gcagccaagg ggatcgcagc gtggcgcgag ccgcggctgg tgcccctgct
     2401 gctggcgacc ggcctcaccg aaggcgcggg gctgttctgg ctcggcgccg cgtggcacgg
     2461 gcgcggcacg ctcgcgttgc tggtggtgtt tggcgcgctg gtgatcgtgc gcatgctcgc
     2521 ctggcgcgca tggcggcagc ggctcgccgc gcacgcggcg ccgcgcgcgc tgaccgcgat
     2581 cgatgcggcg ggcaagacgt tgctggtcgc cggcacactg gttccgctgg cagcgatcgt
     2641 cgtggcgggg gtcgggcagc tcggcgcgcc cgtcacggcg attgcggctg ccgggggact
     2701 cttggccgca gtggccggcg cgtggttcaa gttcacgctc gtcacgcgtg ccggcttcaa
     2761 ccagggcttt gcactcacgc atctgccgat ccgcggcgcg cggcggtgaa gcgatgagcc
     2821 gtcgccgggc cgccccaaga tggctcgctc ccccttgggg ggaccgcgcc gcaggcgcga
     2881 agggggcgct gtgagcaagc ggctgttgag cctgagcgtg aacggccgcg cgcgcgagca
     2941 tgcggtcgcc gatcacgcgc tgctggtcga ctacctgcgc gagacgctcg gcctgactgg
     3001 caccaagcag ggctgcgacg gcggtgagtg cggcgcttgc acggtgctga tcgacggtga
     3061 gccgcggctc tcatgcatca cgctggcggc gacctgcgag ggtgcgcacg tcgagacgat
     3121 cgaaagcctt gccgccagcg gccgcatgag ccggctgcag caggcgtttc acgagaagct
     3181 cggcacgcag tgcggcttct gcacgccggg gatgatcatg gcggcggaag cgctgctgcg
     3241 ccggaacccg cgtccgagcg aagcggagat ccgcgaggcg ctcgccggca acctgtgccg
     3301 ctgcaccggc tacgtgaaga tcatcgagtc ggtgcaggct gcggcggggc agtgacgcga
     3361 tggacacgat cggtcaacgc acgccgctcg tcgacggcat cgagaaagtc accggccgcg
     3421 cccgttacac cgctgacctg ccgttcggcg aaacgctggt cgggcgcatt cgtcggagcc
     3481 atgtcgcgca cggcgtgatc cgccgcatcg atgcgacgaa
//
LOCUS       DBNHBY010000753         6207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1613208, whole genome shotgun sequence.
ACCESSION   DBNHBY010000753 DBNHBY010000000
VERSION     DBNHBY010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1613208"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC16HB_17600"
     CDS             <1..554
                     /locus_tag="AC16HB_17600"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR5608661.1"
                     /translation="LSVLLCAVALTAAPASAQTVQARLTGYHEVPSVASPAHGHFKAK
                     IDADAGMVFWELTYDGLETTAFMSHIHFGQHGVNGGISVWLCGTPNVQPGQVIPPGLP
                     TCPAQGGTLNGSFGASAVIGPAGQLIGAAQLQKLIDAMRAGVTYVNVHTSTASGGVPG
                     GEIRGQIRNAEGRDNRGQGGGKG"
     gene            650..1876
                     /locus_tag="AC16HB_17605"
     CDS             650..1876
                     /locus_tag="AC16HB_17605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5608662.1"
                     /translation="MPKLSRRADSLGTENAFVVLAEVNQLIRAGRDVVSFCIGQPDFP
                     TPTHIQDAAVDAIRAGRHGYTPSAGIDELRAAAARSMSALRGIDIAPDEVVVGAGAKP
                     FIAYAIASVTDHGAGDEVIFPVPGFPIYESQIRANGAVPVALPLRESHHFAFDPAELD
                     ARITPRTKLLILNTPHNPTGGLLTRADLEAIAAVLARHPQIWVYADEIYSKLVYDGAF
                     VSIATLPGMRERTIISDGASKTYAMTGWRIGFTANRTLAPVFTRWITNPESCASQISQ
                     WAALAALTGPQGDADAMRERFRARRDLIVRLLNQVPGFRCALPGGAFYAWPNVTEACA
                     LIGARDSEELRKRLLNEAGVAVLADIHFGPRALFDEFGAGQHIRFSYAASERAIEDGV
                     ARIANFIKRATLPPRK"
     gene            1876..2550
                     /locus_tag="AC16HB_17610"
     CDS             1876..2550
                     /locus_tag="AC16HB_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR5608663.1"
                     /translation="MDTSQARAAILARIRANLRRSAPPQAAELAVADYLAQHPAGPRP
                     QIEGSLPIRFRAMAERSESSVDEVARTTDAPGAVARYLAAQQLEPRAVVWPTMVDLDW
                     NGDGIAVEGRAPRRDESQGSDGVGITGCFCALAETGTLVLLSGPDTHASTHLLPETHI
                     ALVPASRIVPDMEAAFALMRAERGTLPRSVNMVSGPSRTGDIEQTIVLGAHGPFRVHI
                     VVTLQE"
     gene            2557..3537
                     /locus_tag="AC16HB_17615"
     CDS             2557..3537
                     /locus_tag="AC16HB_17615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5608664.1"
                     /translation="MSLRVVVTRRIFPELIEELRTRYALVDNQGDASWTAAELAQRLR
                     DADGALLTSGERVDDALLAACPKLKIASNIAVGYNNIDLAACTRHGVAATNTPDVLNE
                     ATADHAWALLLAAARRVGESERWLRAGHWQRWTFEMFMGADLHATTLGVLGMGRIGRA
                     IARRAQGFGMRVLYHNRQRLPETEEGGAQWVAKEALLRAADHLIVMVPGGEPTRHLIG
                     TAELALMKPTAVLVNLSRGGVVDDDALAAALRDGRLAAAGLDVFEDEPKVHASLLALE
                     SAVLTPHIASSTRATRAAMARLAMRNLDDTLQGRRPAALLNPEVWQHRRR"
     gene            3553..3876
                     /locus_tag="AC16HB_17620"
     CDS             3553..3876
                     /locus_tag="AC16HB_17620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HIR5608665.1"
                     /translation="MSDIKYVRSHNLSLKVAKKVAQKTADDLADEYDLKSEWNGDTLH
                     FHRSGVEGHMHVTAREIDLSVKLGFLLRPFKAKFEQHIERHLDELLAEAKPAKAPSRG
                     GGRKA"
     gene            complement(3879..4931)
                     /locus_tag="AC16HB_17625"
     CDS             complement(3879..4931)
                     /locus_tag="AC16HB_17625"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608666.1"
                     /translation="MKKVAVECIHLGVSYGRNEVLKDITLTIEPGEFFALLGPSGSGK
                     STLLRAIAGFTPPDRGRLRVDGRDITGVPPHARNIGMVFQSYALWPHLTVFDNVAFGL
                     VERRLPRQEIRRRTGAALELVGLHEFAQRRPNQLSGGQQQRVALARTIVIEPQVLLLD
                     EPLSNLDKKLRELMRQELLSLQRKLGITTIFVTHDQEEAMTIADRMAVLDQGMLQQVG
                     APTALFDAPANRFVAEFVGTTNVLVGHFDSARRRFDAQGLGAIPLAAAACAVSVRPHT
                     LALAREARDDAVRVWFDGEIVEREFLGEFTRYAVRAGTVRLIADAPHLATEPLLAVGS
                     KVWIGIDPAEVRPLPV"
     gene            complement(4928..>6207)
                     /locus_tag="AC16HB_17630"
     CDS             complement(4928..>6207)
                     /locus_tag="AC16HB_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608667.1"
                     /translation="LGFTLPLMQDLTGVIFVESLHYFPFILMNLTVALRNIDGAMEEA
                     ALNLGCRGWRLFRRVVFPLALPGYLAGAALVFIKVFDDLGTPLVLGQTNLLAPQAYLR
                     ITQVGLEDPLGYVISVMMIAFSILALWLSARALQGRDYSTLQKGGAGIARRRLATWEA
                     MVAYAAILLVLLVVLSPHLGLAILSFARVWSFSVLPDAYTLANYATVFTDSPHMVTNT
                     LLYCGLAALIDVAIGVAVAYLILRTRLPARQWLDFTASAAIAVPGIVLAIGYLRAFQH
                     FEPFGIPVTQTWLLIMLAYSVRRLPYALRSCMAALQQINVSLEEAAEMLGATGWRVVR
                     RVVIPLMAGGILAGFVSSFITAAVELSATILLVVRDSQAPMSYGIYLYMQSVAGRGPG
                     AALGVLAVLLVAVGTYLSHRLVEQANRIARDAR"
BASE COUNT         1036 a         2149 c         2150 g          872 t
ORIGIN      
        1 ttctatccgt tttgctgtgc gcggttgcgc tgaccgcggc accggccagt gcgcagaccg
       61 tgcaggcgag attgaccggc tatcacgaag tgccgtcggt ggcatcgccc gcccacggcc
      121 acttcaaggc gaagatcgac gccgacgccg gcatggtgtt ctgggagctg acctacgacg
      181 ggctggaaac caccgcgttc atgtcgcaca tccacttcgg gcagcatggc gtcaacggcg
      241 gcatcagcgt gtggctgtgc ggtacgccga acgtgcagcc gggccaggtg attccgcccg
      301 gactgccgac ctgcccggcg cagggcggga cgctcaatgg atcgttcgga gccagcgcgg
      361 tgatcggccc ggcgggacaa ctgatcggcg cggcgcagct gcagaagctg atcgatgcca
      421 tgcgcgccgg cgtcacgtac gtcaacgtgc acacgagcac cgcatcgggc ggtgtgcctg
      481 gcggcgagat ccgcgggcag atccgcaacg ccgaaggacg cgacaaccgc ggccagggtg
      541 gcggcaaagg ctgatcggca aaccacggtt gccgtcaggg gcggtcccgg ccgccctctt
      601 tttttagcgg cgggcggcgc cgcgaggggc ggccatataa tcgccggcca tgcccaaact
      661 ctcgcgacgc gccgacagtc tcggaaccga gaacgcgttc gtcgtactgg ccgaggtcaa
      721 ccagctgatc cgcgccggcc gcgatgtcgt ctcgttctgc atcggccagc cggatttccc
      781 cacgccgacg cacatccagg atgccgcggt cgacgcgatc cgtgccggcc gccacgggta
      841 cacgccgtcg gccggcatcg acgaactgcg cgcggccgcc gcgcgctcga tgtcggcact
      901 gcgaggcatc gacatcgcgc ccgacgaggt cgtggtcggt gcgggtgcga agcctttcat
      961 cgcctacgcg atcgcgtcgg tgaccgacca cggcgccggc gacgaagtga tcttcccggt
     1021 cccgggtttc ccgatctatg agtcacagat tcgtgccaac ggcgcagtgc cggtcgcgct
     1081 gccgctgcgc gaatcgcacc acttcgcatt cgatccggcc gagctcgacg cgcgcatcac
     1141 gccgcgcacg aagctgctga tcctgaacac gccgcacaac ccgacgggcg ggctgctgac
     1201 gcgtgcggac ctggaggcaa tcgccgcggt gctggcgcgc cacccgcaga tatgggtgta
     1261 cgcggacgag atctattcga agctcgtcta cgacggcgct ttcgtgtcga tcgccacgct
     1321 gcccggcatg cgtgaacgca cgatcatctc cgacggcgca tcgaagacct acgcgatgac
     1381 cggctggcgc atcggcttca ccgccaaccg cacgctggcg ccggtattca cgcgctggat
     1441 caccaacccc gagtcgtgcg cgtcacagat cagccagtgg gcggcgttgg ccgcgctgac
     1501 cggcccgcag ggcgacgccg acgcgatgcg cgagcggttt cgcgcgcgcc gcgacctgat
     1561 cgtgcggctg ctgaaccagg tgccgggctt tcggtgcgcc cttcccggtg gcgcgttcta
     1621 cgcctggccc aacgtgaccg aggcatgcgc gctgatcggc gcccgggatt cggaggaact
     1681 gcgcaagcgg ctgctgaacg aggccggcgt cgccgttctg gccgacatcc acttcggccc
     1741 gcgcgcgctg ttcgacgagt ttggcgctgg ccagcacatc cgcttctcct atgcagcgag
     1801 cgaacgggca atcgaggatg gcgtggcgcg gatcgcgaat ttcatcaagc gggcgacgct
     1861 gccgccgcgc aagtgatgga cacctcgcag gcacgcgcgg cgatcctggc gcgaattcgc
     1921 gccaacctgc ggcgctccgc gccgccgcag gccgctgagc tcgcggtcgc cgactacctc
     1981 gcccagcatc cggcgggccc gcggccgcag atcgaaggga gccttccgat ccgcttccgc
     2041 gcgatggccg agcgcagcga gagcagcgtc gatgaagtgg cgcgcacgac cgacgcaccc
     2101 ggtgcggtcg cgcgctacct ggccgcgcag cagctcgagc cgcgcgcggt ggtctggccg
     2161 acaatggtag acctggactg gaatggggac ggcatcgcgg tcgaggggcg cgcgccgcgc
     2221 cgcgacgaat cgcagggctc cgacggcgtc ggcatcaccg gctgcttctg cgcgctggcc
     2281 gagaccggca cgctggtgct gttgtccggc cccgacacgc acgcgtcgac gcacctgctg
     2341 cccgaaacgc acatcgcgct ggtgccggcg tcgcggatcg tgcccgacat ggaagccgcg
     2401 ttcgcgctga tgcgcgcgga gcgcggcacg ctgccgcgct cggtcaacat ggtatcgggc
     2461 ccgtcgcgca ccggcgacat cgagcaaacg atcgtgctcg gcgcgcacgg gccgttccgc
     2521 gtgcacatcg tcgtcacgct gcaggagtga aggcgcatga gcctgcgcgt cgtcgtgacc
     2581 cgtcgcatct ttcccgagct gatcgaggaa ctgcgcacgc gctacgcgct ggtcgacaac
     2641 caaggcgacg catcctggac cgcggccgag ctcgcgcagc gcctgcgcga tgccgacggc
     2701 gcgctgctga cctcgggcga gcgcgtcgac gacgccctgc tcgccgcgtg cccgaagctg
     2761 aagatcgcga gcaacatcgc agtgggctac aacaacatcg acctcgccgc ctgcacccgg
     2821 cacggcgtgg cggccaccaa cacgcccgac gtgctgaacg aagcgacggc cgaccacgcg
     2881 tgggcattgc tgctggcggc ggcgcgccgc gtcggtgaat ccgagcgctg gctgcgggcc
     2941 gggcattggc agcgctggac gttcgagatg ttcatgggcg ccgatctgca cgcgaccacg
     3001 ctcggcgtgc tcggcatggg ccgcatcggc cgcgcgatcg cgcgccgcgc acagggcttc
     3061 ggcatgcgcg tgctgtacca caaccgccag cggctgccgg aaacggaaga gggcggcgcg
     3121 caatgggtgg cgaaggaggc cctgctgcgc gcggccgatc acctgatcgt catggtcccc
     3181 ggcggcgagc cgacgcgcca tctgatcggc accgcggaac tggcactgat gaagccgacc
     3241 gcggtgctgg tgaacctgtc gcgcgggggc gtggtcgacg acgatgcgct cgcggccgcg
     3301 ctgcgcgacg gccggctcgc cgcggccggg ctcgacgtgt tcgaggacga gcccaaggtg
     3361 cacgccagcc tgctcgcgct ggaaagcgcg gtgctcacgc cgcacatcgc gtcgagcacg
     3421 cgcgccacgc gcgcggcgat ggcgcggctg gcgatgcgca acctcgacga cacgctgcag
     3481 gggcgccgcc cggcggcgct gctgaacccc gaggtgtggc agcaccgacg ccgctagagc
     3541 cggagccgac cgatgtccga tatcaagtac gtgcgcagcc acaatctgtc gctgaaggtg
     3601 gcgaagaagg tggcacagaa gaccgccgac gacctcgccg acgaatacga cctgaagagc
     3661 gaatggaacg gcgacacgct gcacttccat cgcagcggcg tcgagggcca catgcacgtg
     3721 accgcgcgcg agatcgacct gagcgtgaag ctcggcttcc tgctcaggcc gttcaaggcg
     3781 aagttcgagc agcacatcga gcgccacctg gacgaactgc tcgccgaagc gaagccggcc
     3841 aaggcgcctt cccgcggcgg cggccgcaag gcctgacgtc agaccggcag ggggcggacc
     3901 tcggcgggat cgataccgat ccacaccttg gagccgaccg cgagcagcgg ctcggtcgcc
     3961 aaatgcggcg catccgcgat caggcgcacc gtgccggcgc gcaccgcgta gcgggtgaac
     4021 tccccgagga actcgcgctc gacgatctcg ccgtcgaacc agacgcggac tgcgtcgtcg
     4081 cgcgcttcgc gcgccagggc cagcgtatgc ggacgcaccg aaacggcgca cgcggccgcg
     4141 gcgagcggta tcgcgccaag cccctgcgcg tcgaagcggc ggcgcgccga atcgaagtgc
     4201 ccgaccagca cgttggtcgt gccgacgaac tcggcgacga atcggttcgc cggcgcatcg
     4261 aagagggcgg tcggcgcacc gacctgctgc agcatgccct ggtcgagcac ggccatgcgg
     4321 tcggcgatcg tcatcgcctc ctcctggtcg tgcgtgacga agatcgtcgt gatgccgagc
     4381 ttgcgctgca atgacagcag ctcctgccgc atcagttcgc gcagcttctt gtcgagattc
     4441 gacagcggct cgtcgagcag cagcacctgc ggctcgatca cgatggtgcg cgccagcgcg
     4501 acgcgctgct gctggccgcc ggagagctgg ttcggccgcc gttgcgcgaa ttcgtgcaag
     4561 ccgacgagtt cgagcgcggc tccggtgcgg cggcggatct cctgcctcgg cagccgccgc
     4621 tcgacgagtc cgaacgcgac gttgtcgaac accgtgaggt gcggccacag cgcgtagctc
     4681 tggaacacca tgccgatgtt gcgcgcgtgc ggcggcacgc cggtgatgtc ccgcccgtcg
     4741 acccgcagtc gtccgcggtc cggcggggtg aagccggcga tcgcgcgcag cagcgtgctt
     4801 ttgccggagc cactggggcc gagcagcgca aagaattcgc ccggctcgat ggtgagtgtg
     4861 atgtccttca gcacttcgtt gcggccgtag gagacgccga gatgaatgca ttcgacggcg
     4921 accttcttca tcgtgcgtcc cgtgcaatgc ggttggcctg ctcgaccagg cgatgcgaca
     4981 gatacgtgcc cacggcgacc agcagcaccg ccagcacgcc gagcgccgct cccggcccgc
     5041 gcccggcgac cgactgcatg tacaggtaga tgccgtagct catcggtgcc tgcgaatcgc
     5101 gaacgaccag caggatcgtc gccgacagtt cgaccgccgc ggtgatgaaa ctcgacacga
     5161 agccggcgag gatgccgcct gccatcagcg ggatcaccac gcgccgcacg acccgccagc
     5221 ccgtcgcacc gagcatttcg gcggcttcct ccaggctgac gttgatctgc tgcagggcgg
     5281 ccatgcacga gcgcagcgca tacggcagcc ggcgcaccga gtacgccagc atgatcagca
     5341 gccatgtctg cgtgaccggg ataccgaacg gctcgaagtg ctggaaggca cgcaggtagc
     5401 cgatcgccag cacgattccg ggcaccgcga tcgccgcgct cgcggtgaag tcgagccact
     5461 gccgcgccgg cagccgcgtg cgcaggatca ggtaagcgac cgcaacgccg atcgccacgt
     5521 cgatcagtgc cgccagtccg cagtacagca gcgtattggt gaccatgtgc gggctgtcgg
     5581 tgaacacggt cgcgtaattg gcgagcgtgt aggcgtccgg cagcaccgag aagctccaga
     5641 cgcgtgcgaa cgacaggatc gcgaggccca ggtgcggcga cagcacgacc agcagcacca
     5701 gcaggatcgc ggcatacgcg accatcgctt cccaggtggc gaggcgccgc ctggcgatgc
     5761 cggcgccgcc cttctgcagc gtcgagtagt ccctgccctg cagtgcgcgc gcggacagcc
     5821 acagcgccag gatcgagaaa gcgatcatca tcacgctgat cacgtaaccg agcggatcct
     5881 cgagtccgac ctgcgtgatg cgcaggtagg cctgcggcgc gagcaggttg gtctgcccca
     5941 gcaccagcgg cgtgccgagg tcatcgaaga ccttgatgaa caccagcgcg gcgccggcga
     6001 gatagcccgg caatgcgagt gggaacacca cgcgccggaa caggcgccag ccacggcagc
     6061 cgaggttcag cgccgcttcc tccatcgcgc cgtcgatatt gcgcagcgcg acggtcaggt
     6121 tcatcaggat gaacggaaag tagtgcaggc tctcgacgaa gatcacgccg gtcaggtcct
     6181 gcatcagcgg cagcgtgaag ccgagat
//
LOCUS       DBNHBY010000754         1803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1613484, whole genome shotgun sequence.
ACCESSION   DBNHBY010000754 DBNHBY010000000
VERSION     DBNHBY010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1613484"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC16HB_17635"
     CDS             complement(<1..368)
                     /locus_tag="AC16HB_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608668.1"
                     /translation="MSARLPDTVAADPLLRFAVVAPVVIRPQHADDGALLAEFVRGLS
                     PVARYQRFHMTINDLSAPLLERLTRIAPPQEMALLATLDAGAREIVLGEARFGAADDQ
                     SEAREFALAVGDAWQRVGIGA"
     gene            complement(349..618)
                     /locus_tag="AC16HB_17640"
     CDS             complement(349..618)
                     /locus_tag="AC16HB_17640"
                     /inference="COORDINATES: protein motif:HMM:NF022592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HIR5608669.1"
                     /translation="MDSNLKFAMNQLQRGHYVRLNDAVGRTVAVFKGMVWITQDRDPR
                     DMFVGRGETFRIDRPGVVLVEAIDDASLIVFEPRTAEVSHERALA"
     gene            complement(986..>1803)
                     /locus_tag="AC16HB_17645"
     CDS             complement(986..>1803)
                     /locus_tag="AC16HB_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5608670.1"
                     /translation="GDPTQLHQVVMNLCSNAVQAIRAAGTVAVSIDTLESAEPLCLTT
                     GELAPGYYVRLRVSDTGTGIAPQVIERIFDPFFTTKEVGVGTGLGLSLVHGIVTDLGG
                     GINVESDVGAGSTFTVYLPWQGCARLPERVDEPIAHGAGQTILLVDDEVPLIQLGEEM
                     MAELGYEPVGFTSSRQALESFRAEPERYDVVLSDEAMPGMTGSELAAEIHRIRPEIPV
                     VLMSGYATAELAARARAAGVIEVLAKPLVSRDVARSLASALRGVPTETAATAR"
BASE COUNT          283 a          640 c          610 g          270 t
ORIGIN      
        1 gcgccgatgc cgacgcgttg ccaggcatcg ccgaccgcga gcgcgaactc gcgcgcttcg
       61 gactggtcat cggcagcgcc gaagcgtgcc tcgccgagca cgatttcgcg cgcgccggcg
      121 tcgagcgtgg ccagcagcgc catctcctgc gggggcgcga tccgcgtcag gcgttcgagc
      181 aagggcgccg acagatcgtt gatcgtcatg tgaaagcgct ggtagcgcgc gaccggcgac
      241 aggccgcgca cgaactccgc cagcagcgcg ccgtcgtccg cgtgctgcgg ccggatgacg
      301 accggagcga ccacggcgaa gcgcagcagc gggtcggcgg caacggtgtc aggcaagcgc
      361 gcgctcatgg ctcacctccg cagtccgcgg ctcgaacacg atcaggctcg cgtcgtcgat
      421 tgcctcgacc agcaccacgc cggggcgatc gatgcgaaac gtttcgccgc ggccgacaaa
      481 catgtcgcgc gggtcgcgat cctgcgtgat ccagaccatg cccttgaaca cggccacggt
      541 gcggccgacg gcatcgttca ggcgcacgta gtgcccgcgc tgcagctggt tcatcgcgaa
      601 tttcaggttc gagtccattt cgcacactcc tcgcccggac cctgccgggc gttcgttcac
      661 acggcgagcg ccaacggcgc caccaccatc accaccatcc agctcaccga cgccacggcg
      721 gcagccgccg ccagcgcgag ccgttccttc gtcgatcggg aggtcatgtc caactccatg
      781 cggttgtttg aacacaagcg catggtgagg cgcggcagtt gtgcgccgat ttcagttcgg
      841 cgcgccgcga tttcagttgc aatcggcgca gcggcccacc gtggctctgc gccacgctcg
      901 gtcgactccg atgaattgcg aggtggtcgc aggctccgat ggcaagaggc gccgcgcgac
      961 cgcgagaggc ggccgagccg ccgtttcagc gcgcggtcgc tgccgtttcg gtcggaacgc
     1021 cgcgcagcgc gctcgccagg ctgcgcgcaa cgtcgcgcga caccagcggc ttggcgagca
     1081 cctcgatcac gccggcggcg cgcgcgcgcg ccgcgagctc cgcggtggcg tatccgctca
     1141 tcagcacgac cgggatctcg ggccggatgc gatggatctc ggccgcgagt tcggagcccg
     1201 tcatgccggg catcgcctcg tccgacagca cgacgtcata tcgctccggc tcggcgcgaa
     1261 aagactccag cgcctgtcgg ctggaggtga agccgaccgg ctcgtagccg agctcggcca
     1321 tcatctcctc gccgagctga atcaacggca cttcgtcgtc gaccagcagg atcgtctgcc
     1381 ccgcgccgtg ggcgatcggc tcgtcgacgc gctccggcag ccgcgcgcag ccctgccacg
     1441 gcaggtacac ggtgaaggtc gagccggcac cgacgtcgct ttcgacattg atgccgccgc
     1501 cgaggtcggt cacgatgccg tggaccagcg acaggccgag tccggtgccg acgccgacct
     1561 ccttggtcgt gaagaacggg tcgaagatgc gttcgatcac ctgcggcgcg ataccggtgc
     1621 cggtatcgga cacgcgcagc cgcacgtagt agccgggcgc cagttccccg gtggtaaggc
     1681 acaggggttc cgcggattcg agcgtgtcga tcgacaccgc caccgtgccg gccgcgcgga
     1741 tcgcctgtac cgcgttcgag cacaggttca tcaccacctg gtgcagctgg gtcggatcgc
     1801 cga
//
LOCUS       DBNHBY010000755         2298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1885108, whole genome shotgun sequence.
ACCESSION   DBNHBY010000755 DBNHBY010000000
VERSION     DBNHBY010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1885108"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="AC16HB_17650"
     CDS             complement(<1..597)
                     /locus_tag="AC16HB_17650"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5608671.1"
                     /translation="MDRITAMQVFTEVADRASLTDAARALDISRAMASRYLESFERWL
                     GVRLLHRTTRRVSLTDAGEEALPRCRQMLELSRDLQTVAGARSAEPAGKLRLTTSTSF
                     AEAFLADVVTDFLSRYPQAQVELIALERSVNLVEERIDLAVRIGNVLDDSLVARRLGL
                     CRSVVCASPAYIKANGFPSVPDDLRNHRCITHAYVGRTE"
     gene            703..1584
                     /locus_tag="AC16HB_17655"
     CDS             703..1584
                     /locus_tag="AC16HB_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5608672.1"
                     /translation="MMKPLLPLLTSLTLGVVANEAAGQASPPPLVLHVYNADSGSFHV
                     NAVLVSGKADAVLIDTGFTQADAHRIAAMILDSRKTLKAIYISQADPDFYFGAGVLKR
                     LFPEATLIAIAPTVKKIQATLPTKLQVWGPRLGVNAPKDVPVPEAMTGNTILLEGQTL
                     EIRGLDGSLPHRSYVWIPSLKAIVGGVNAFAGLHVWTADTQTVQERADWIGKLGSMAA
                     LQPTTVVPGHMLPGQKQDTSQLAYTQSYLRRFESELAKAPHSAALIEAMKSAYPTAGL
                     MVALEIGAKVSKGEMKW"
     gene            complement(1606..1818)
                     /locus_tag="AC16HB_17660"
     CDS             complement(1606..1818)
                     /locus_tag="AC16HB_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608673.1"
                     /translation="MLTRFMQRPGLWMPVYLLLAAICTVATAEAASRADEGKVEAIEI
                     AYSAPDNNPVNKGTVIGGIAAVHAAG"
     gene            complement(1859..>2298)
                     /locus_tag="AC16HB_17665"
     CDS             complement(1859..>2298)
                     /locus_tag="AC16HB_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608674.1"
                     /translation="SRSGGSEGFGFAVTSNAAREILLDARPIWTGLSVVPLSGELARL
                     LNLPQAAGLLVMHAAEDSPGAQVGLRAGTTPAVIGGVNLTLGGDIILAVAGMPIEANA
                     ANYRRIHDRLRRAQTGEQIPIVVLRAGRIVELSGANMKAAGSN"
BASE COUNT          423 a          737 c          714 g          424 t
ORIGIN      
        1 ctcggttcgg cccacgtagg cgtgggtgat gcagcggtgg tttcgaaggt cgtccgggac
       61 gctgggaaaa ccgtttgcct tgatgtacgc cggcgacgcg cagacaacgg agcggcacaa
      121 acctagcctg cgcgcaacca gcgagtcgtc gagcacgttg ccgatccgca cggccaggtc
      181 gatgcgttct tccaccagat tgacagaacg ttccagcgca attagttcga cttgcgcttg
      241 cggataccgg gaaagaaagt ccgtcaccac gtcggccagg aaggcctcgg cgaaagacgt
      301 gctcgtcgtc aatcgcagct tgcccgccgg ttccgcgctc cttgcgcctg ccacggtctg
      361 tagatcgcgc gagagttcga gcatctggcg gcagcgcggc agcgcttcct cgccggcatc
      421 ggtgaggctg actcgcctgg tcgttcggtg cagcagccgt actccgagcc agcgctcgaa
      481 gctctccaga taacgcgagg ccatggcgcg tgagatgtcc agtgccctgg cagcgtcggt
      541 caggctggcg cggtctgcca cttcggtgaa cacctgcatg gcggtgattc ggtccatcga
      601 ttagttcgat cgggtagaca aagtgtcgac cagtctactg tttatcgacg atttggtaac
      661 aaatagagtg gcgccatctt ccgaccactg cgagcgattc caatgatgaa gcctctcctt
      721 ccactgctta caagcctcac tctcggcgtt gtcgccaacg aggccgccgg ccaggcgagt
      781 ccgcctccgc tcgtccttca cgtctacaac gccgactcag gcagcttcca cgtcaacgcc
      841 gtcctggtgt ccggcaaggc ggacgccgta ctcatcgata ccggattcac tcaggccgat
      901 gcgcaccgga tcgcggcgat gattctcgac agccgcaaga cgctgaaggc catctacatc
      961 agccaggccg acccggactt ctacttcggc gcgggtgtac tgaagcgact gtttcccgaa
     1021 gcgacactga tcgccattgc gcctaccgtg aagaagattc aggccacgct ccccacgaag
     1081 ctgcaggtgt ggggcccgcg gctcggcgtc aatgcgccga aagacgttcc ggtgcccgag
     1141 gccatgaccg gaaacacaat cctgctggaa ggccagacgc tcgaaatcag agggctggat
     1201 ggttcgcttc cgcaccgcag ctacgtatgg attccttctt tgaaggccat tgtgggcggg
     1261 gtgaatgcct ttgccggcct gcatgtgtgg acggcggaca cgcagaccgt gcaggagaga
     1321 gccgactgga tcggcaagct gggctcgatg gctgcgcttc agcccaccac tgtcgttccc
     1381 ggccacatgc tgccagggca aaagcaggac acttcccagc ttgcctacac gcagtcctac
     1441 ctgcgccgct ttgagtcaga actggccaag gcgccgcatt cagccgcgct gatcgaggcg
     1501 atgaagtcgg cctacccgac ggcgggcctg atggtggcgc tcgaaatcgg cgccaaggtc
     1561 agcaagggcg aaatgaaatg gtgatgcagg tcgtcagagc ggaccctagc cagcagcgtg
     1621 gacggctgcg atgccgccga tcacggttcc cttattgaca gggttgttgt cgggcgcgct
     1681 ataggcgatc tcgatggcct cgactttgcc ctcgtcggcg cggctggcgg cttcggccgt
     1741 cgccacggtg cagatagccg ctaggagcag gtacacgggc atccagagac cgggccgttg
     1801 catgaaacga gtcagcactt catcttcttt gcataatgtt ggtgcacgaa tatcgtcatc
     1861 aattcgagcc ggccgctttc atgttcgcac cgctgagttc gacgatccgg ccggcgcgca
     1921 gaacgacgat ggggatctgc tcccctgtct gcgcccgccg cagccgatcg tggatcctgc
     1981 ggtagttggc ggcattcgcc tctatcggca tccctgcaac cgccaagatg atgtccccgc
     2041 cgagcgtcag gttcacgccc ccgatcaccg cgggcgtggt gccggctctg aggcccacct
     2101 gcgccccggg cgagtcttcc gccgcgtgca tcaccagcaa cccggccgcc tgcggcaggt
     2161 tgagaagccg agccagttcc ccggacagcg ggacgacgga cagccccgtc cagatgggtc
     2221 gcgcatcgag caggatttcc ctcgcggcgt tggaagtcac ggcaaagccg aatccttcgg
     2281 agccccccga gcgactga
//
LOCUS       DBNHBY010000756         5208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_869111, whole genome shotgun sequence.
ACCESSION   DBNHBY010000756 DBNHBY010000000
VERSION     DBNHBY010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_869111"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="AC16HB_17670"
     CDS             <1..405
                     /locus_tag="AC16HB_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR5608675.1"
                     /translation="QQVLEASVSDEIDVDSLLETDEALSFRRPGIGPDALRKLRRGEW
                     TIQDQLDLHGHRVDEARAALVEFLRDVLKRGLRCVRIVHGKGLGSKDRTPVLKSKVRA
                     WLVQRDEVIAFCQARPADGGSGALVVLLRPSA"
     gene            complement(409..747)
                     /locus_tag="AC16HB_17675"
     CDS             complement(409..747)
                     /locus_tag="AC16HB_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5608676.1"
                     /translation="MKQITAVIKPFKLDEVREALADVGVTGMTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIDAVVPEAMVEQAIEAIMKAARTGKIGDGKIFVRDVEQVIRI
                     RTGETGNDAI"
     gene            complement(775..2406)
                     /locus_tag="AC16HB_17680"
     CDS             complement(775..2406)
                     /locus_tag="AC16HB_17680"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5608677.1"
                     /translation="MSSHVRVAVAQINCTVGDFEGNSRRILDMARRAAEAGADILLTP
                     ELSLSGYPPEDLLLRRAFYAASDDALEKLAAQLLQWPKLRVIVGHPLQREGRRFNAAS
                     LLFDGALHGTYCKHDLPNYDVFDEQRYFTPDNRPFVFDVKGTRFGINICEDTWFPYAP
                     ECARSAGAQVLLVPNGSPFHLGKQAQRLDEMRANVSQSGLALVYANLVGGQDELVFDG
                     ASFVLDGHGKLAHMAPQFEEALLLTEFVGGVPEHGACADLGSTESQAYRALVLGVHDY
                     VTKNGFPGAIIGFSGGIDSALTLAIACDALGHDKVRAVMMPSQFTRDISWLDSREMAQ
                     RLNVRYDELPIKPIFDGFLAALAQEFRGLPWNTAEENIQARIRGTLLMTLSNKYGSIV
                     LTTGNKSEMAVGYATLYGDMAGGFAVIKDIRKTLVYALSRYRNSISAVIPERIITRAP
                     SAELKAGQVDQDSLPPYDVLDGIIQLYMEEGHPIDEVIAAGYPESVVRNVVRMIRMNE
                     YKRRQAPVGIRVTHRGFGRDWRYPITNRFREDNGG"
     gene            2418..3581
                     /locus_tag="AC16HB_17685"
     CDS             2418..3581
                     /locus_tag="AC16HB_17685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5608678.1"
                     /translation="MALAQDYRTQIIGDLSAVGRAAWDALLAADADANPFLCYEFLHA
                     LHESGAAVPATGWAPNYLSLWRGDRLAGAVPLYAKSHSYGEYVFDWGWAEAYQRHGLA
                     YYPKWLAAVPFTPVGGARLIAIDEDARVRLADALVAAARASTLSSLHVLLAPPAQIEL
                     LSKRGMLIRRGVQYHWRNAGYADFDDFLARMLQPKRKKIRAERRKVAEQGVALVRKVG
                     AEISDADWQFFHRCYRATYQAHWSTPYLNLEFFRRIGAAMPANLLLVIAARGPRRIAA
                     ALGVFDAHHLYGRYWGAVEHVPCLHFECCYYQMIDFAIERRLRVFEGGAQGAHKIARG
                     LDPVPTFSAHWLAHAGMSAAVRRFLAQEGESVAHAIDELNEHTAFRAATPRHE"
     gene            3703..4560
                     /locus_tag="AC16HB_17690"
     CDS             3703..4560
                     /locus_tag="AC16HB_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5608679.1"
                     /translation="MRPLHLAAALVMVAIWGFNFIVIRWGLNDVPPLTLTCVRFVLAA
                     VPALFFVARPRTPWWHLAAYGLFAFALQFSFLFTGIAVGMPTGLASLVIQVQAFFTIG
                     LAALIDRERPRPPQLAGALLAAGGIVLVGIHLPGGGTRVGLLFVLAAAASWATANLIV
                     KGVRGEPLAVVVWASAFAALLMLPVALLIERPERMVQATISLSAAQWVGIAFQAWPTT
                     LLAFGIWAWLLRRYPAALIAPFALLVPIVGMSCAVWLLGEPAHWWQLAGAALVLGGLA
                     LNVASYRRA"
     gene            complement(4557..5117)
                     /locus_tag="AC16HB_17695"
     CDS             complement(4557..5117)
                     /locus_tag="AC16HB_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5608680.1"
                     /translation="MSCAAPIASRTLVDYWLGDLDAQSEESVERHLLGCASCSSELQQ
                     WAEFGDALRALARGGAVAAVVSGEFVARVAQRGLRVREYRVPCNGSVQCTVAPDDDML
                     ISRLAAPLAGIARLDLLVYDEGDAAPTRLVDIPFRADAGEVVLAPPIERIRALPASTS
                     RMRLVAVDDGRERVLGDYTFLHRPWS"
BASE COUNT          731 a         1817 c         1769 g          891 t
ORIGIN      
        1 cagcaggtgc tggaggcgtc ggtgtcggac gagatcgacg tcgactcgct gctcgagacc
       61 gacgaggcgc tttcatttcg ccgccccggg atcgggccgg acgcgttgcg caagctgcgc
      121 cgcggcgaat ggacgatcca ggatcagctc gatctgcacg gccatcgcgt cgacgaggca
      181 cgcgccgcgc tggtcgagtt cctgcgcgat gtgctcaagc gcggcctgcg ctgcgtgcgc
      241 atcgtgcacg gcaagggact ggggtcgaag gaccgcacgc cggtcctgaa gtccaaggtg
      301 cgcgcgtggc tggttcagcg cgacgaggtg atcgccttct gccaggcgcg gcccgccgat
      361 ggcggcagcg gcgcgctggt ggtgctgctg cgcccttccg cctgacgctc agatcgcgtc
      421 gttgcccgtc tcgccggtgc ggatgcggat cacctgctcc acgtcgcgca cgaagatctt
      481 gccgtcgccg atcttgccgg tgcgcgccgc cttcatgatc gcctcgatcg cctgctcgac
      541 catcgcctcc ggaaccaccg cgtcgatctt caccttgggc aggaagtcga ccacgtactc
      601 ggcaccgcgg tacagctccg tgtgaccctt ctgccggccg aagcccttga cctcggtgac
      661 cgtcatgccg gtgacgccga catcggccag cgcctcgcgc acttcgtcga gcttgaacgg
      721 tttgatgacc gcggtgatct gtttcatggt gagccccctc aactggcccg gattctagcc
      781 gccgttgtcc tcgcggaagc ggttggtgat cggatagcgc cagtcgcggc cgaatccgcg
      841 atgggtcacg cggatgccga ccggcgcctg gcgccgcttg tattcgttca tgcggatcat
      901 ccgcaccacg ttccgtacga cggactcggg atagccggcg gcgatcacct cgtcgatggg
      961 gtgaccctct tccatgtaga gctggatgat gccgtcgagc acgtcgtacg gcggcaggct
     1021 gtcctggtcg acctggccgg ccttcagctc cgcgctcggc gcgcgcgtga tgatgcgctc
     1081 cgggatcacc gccgagatcg aattgcgata gcgcgacagc gcgtacacca gcgtcttgcg
     1141 gatgtccttg atcacggcga agccgcccgc catgtcgccg tacaacgtcg cgtagccgac
     1201 cgccatctcg ctcttgttgc cggtcgtcag cacgatcgag ccgtacttgt tcgacagcgt
     1261 catcagcagc gtgccgcgaa tgcgcgcctg gatgttctcc tccgcggtgt tccacggcag
     1321 gccgcggaac tcctgcgcca gcgcggcgag aaagccgtcg aagatcggct tgatcggcag
     1381 ttcgtcgtag cgcacgttca ggcgctgcgc catctcgcgc gagtcgagcc acgagatgtc
     1441 gcgcgtgaac tgcgacggca tcatcaccgc gcgcaccttg tcgtggccga gcgcatcgca
     1501 cgcgatcgcc agcgtcagcg ccgaatcgat gccgcccgaa aagccgatga tcgcgcccgg
     1561 aaagccgttc ttggtcacgt aatcgtgcac gccgagcacc agcgcgcgat aggcctggct
     1621 ttcggtcgaa ccgaggtccg cgcacgcgcc gtgctcgggc acgccgccga cgaactccgt
     1681 gagcagcagc gcttcctcga actgcggcgc catgtgcgcg agcttgccgt gcccgtcgag
     1741 cacgaacgaa gcgccgtcga acaccagttc atcctgcccg ccgacgagat tcgcgtagac
     1801 cagcgccagc ccgctctggc tcacgttggc gcgcatctcg tcgagccgct gtgcctgctt
     1861 gccgagatgg aacggcgagc cgttcggcac cagcagcacc tgggcgcccg ccgagcgcgc
     1921 gcattcgggc gcatagggga accaggtgtc ctcgcagatg ttgatgccga agcgcgtgcc
     1981 cttcacgtcg aacacgaaag ggcggttgtc gggcgtgaag tagcgctgct cgtcgaagac
     2041 gtcgtagttc ggcaggtcgt gcttgcagta ggtgccgtgc agcgcgccgt cgaacagcaa
     2101 cgacgccgcg ttgaagcgcc gcccctcgcg ttgcagcggg tggccgacga tcacgcgcag
     2161 cttgggccat tgcagcagtt gtgcggcgag cttttcgagc gcgtcgtcgc tcgctgcgta
     2221 gaatgcgcgc cgcagcagca ggtcctcggg cggatagccg ctgagcgaca gctcgggagt
     2281 cagcaggatg tcggcgccgg cctcggcggc cctgcgcgcc atgtcgagaa tccgccggct
     2341 gttgccttcg aaatcgccga ccgtgcagtt gatctgagcg acggcgaccc ggacgtgcga
     2401 actcatcgga tgggtgtttg gcgttggcgc aggattatcg cacgcagatc atcggggacc
     2461 tgagcgcagt cggccgcgcc gcgtgggacg cactgctcgc cgccgatgcc gacgccaatc
     2521 cgttcctgtg ctacgagttc ctgcacgcgc tgcacgagag cggcgcggcg gtgcctgcca
     2581 ccggctgggc gccgaactac ctctcgttgt ggcgcggcga ccggctcgcg ggcgccgtgc
     2641 cgctgtacgc gaagtcgcac tcctatggcg agtacgtgtt cgactggggc tgggccgagg
     2701 cctaccagcg ccacggcctc gcctattacc cgaagtggct tgccgcggtg ccgttcacgc
     2761 ccgtcggcgg cgcgcgcctg atcgccatcg acgaggatgc gcgcgtgcgg ctcgccgacg
     2821 cgctggttgc cgccgcgcgc gcgagcacgc tgtcgtcgct ccatgtgctg ctggcgccgc
     2881 ccgcgcagat cgaactgctg tcgaagcgcg gcatgctgat ccggcgcggc gtgcagtatc
     2941 actggcgcaa tgcgggctac gcggacttcg acgacttcct cgcgcgcatg ctgcaaccga
     3001 agcgcaagaa gattcgcgcc gagcgcagga aggtcgccga gcagggcgtc gcgctcgtgc
     3061 gcaaggtcgg cgccgagatc agcgacgccg actggcagtt cttccaccgc tgttatcgcg
     3121 cgacctacca ggcgcactgg tccacaccgt acctgaacct cgaattcttc cgccgtatcg
     3181 gcgcagcgat gccggcgaat ctgctgctgg tcatcgccgc gcgcgggccg cggcggatcg
     3241 ccgccgcgct cggcgtcttc gatgcgcacc atctttacgg ccgctactgg ggcgcggtcg
     3301 agcatgtgcc ctgcctgcac ttcgagtgct gctactacca gatgatcgac ttcgcgattg
     3361 agcgccggct gcgggtcttc gagggcggcg cgcagggcgc acacaagatc gcgcgcggcc
     3421 tcgacccggt gccgaccttc tccgcgcact ggctcgcgca cgcgggcatg tctgcagcgg
     3481 tgcgacgctt cctcgcgcag gaaggcgaga gcgtcgcgca cgcgatcgac gaactgaacg
     3541 agcacacggc ctttcgcgcc gcgaccccgc ggcacgaatg atcccgcgtc accgcgccgc
     3601 tttcctgtcg atggttccga tccgcggctc ggcgccgcgc gccagcggcg acgcagaggt
     3661 ctcacgtaac ccccttcctt tcggtctgca taggtcactc gcatgagacc tctgcatctc
     3721 gcggccgcct tggtcatggt ggcgatctgg ggcttcaact tcatcgtcat ccgctggggc
     3781 ctgaacgacg tgccgccgct gacgctcacc tgcgtgcgct tcgtactggc ggcagtgccg
     3841 gcgctcttct tcgtcgcgcg tccgcgcacg ccgtggtggc acctcgccgc ctacggcctg
     3901 ttcgcgttcg cgctgcagtt ctcgttcctg ttcaccggca tcgcggtcgg catgccgacc
     3961 gggctcgcgt cgctggtgat ccaggtgcag gcgttcttca cgatcggact ggcggcgctg
     4021 atcgaccgtg agcggccgcg cccgccgcaa ctcgcgggtg cgctgctcgc cgccggcggc
     4081 atcgttctgg tgggaatcca cctgcctggc ggcggcaccc gcgtcggact gctgttcgtg
     4141 ctcgcggcgg cagcgtcgtg ggcaaccgcg aatctcatcg tcaagggcgt gcggggtgag
     4201 ccgctcgcgg tcgtcgtctg ggccagcgcg ttcgcggcgc tgctgatgct gccggtcgcg
     4261 ctgctcatcg aaagaccgga gcgcatggtc caggcaacca tttcgctcag tgccgcgcag
     4321 tgggtcggga tcgctttcca ggcgtggccg acgacgctgc tcgcgttcgg catctgggcc
     4381 tggctgttgc ggcgctatcc ggccgcgctg atcgcgcctt tcgcgctgct ggtgccgatc
     4441 gtcggcatgt cgtgcgcggt atggctgctc ggcgagcccg cgcactggtg gcaactcgcc
     4501 ggcgccgcgc tcgtcctagg cgggctggcg ctgaacgtcg cgagctatcg gcgcgcctag
     4561 ctccagggcc ggtgcaggaa cgtgtagtcg ccgagcacgc gctcgcggcc gtcgtcgacc
     4621 gcgaccaaac gcatgcgcga agtcgacgca ggcagcgccc ggatgcgttc gatcggcggc
     4681 gccagcacga cctctcccgc gtcggcgcgg aacgggatgt ccaccagccg cgtcggtgcg
     4741 gcatcgcctt cgtcgtacac cagcaggtcg agcctggcga tgccagccag cggggcggcc
     4801 agccgcgaaa tcagcatgtc gtcgtcgggc gcgacggtgc attgcacgga cccgttgcac
     4861 ggcacgcgat actcgcgcac gcgcaggccg cgctgcgcga cacgcgcgac gaattcaccg
     4921 ctgacgacgg cggcgaccgc accgccgcgc gccagcgcgc gcagggcgtc gccgaactcg
     4981 gcccactgct gcagctcgct gctgcaggac gcgcagccga gcagatgccg ctcgacgctc
     5041 tcctcgctct gcgcgtcgag gtcgccgagc cagtagtcga ccaacgtccg cgatgcgatc
     5101 ggtgcggcac agctcatgag gctcctccgg tcacgcactc gcgcagccgc tgcagcgcgc
     5161 ggtggcggat cacgcgcacg ttcgcctcgg tcagcgccag ttccgccg
//
LOCUS       DBNHBY010000757        32990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_396294, whole genome shotgun sequence.
ACCESSION   DBNHBY010000757 DBNHBY010000000
VERSION     DBNHBY010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 32990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32990
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_396294"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="AC16HB_17700"
     CDS             complement(<1..919)
                     /locus_tag="AC16HB_17700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR5608681.1"
                     /translation="MAAQNNAALPEGIEVGGYRIVKKISSGGFSIVYLATDEHGNSVA
                     IKEYMPAALAKRGPGELAPYVAPENLNTYRIGLKCFFEEGRALASIYHPNIVRVLNFF
                     RANDTVYMVMHYESGRSLQEHVLRNRSKDQKDVLSERFIRRVFTQVMNGLREVHSNRL
                     LHLDVKPANIYLRMDGTPILLDFGAARQTLQRDISKTYPMYTPGFAAPELYKRDAELG
                     PWTDVYSIGACIYACMAGMPPQESDARLKEDKMPQVLRAFRGLYSSELLSMVERCLRL
                     NSLERPQTIYAVQKELINNVPERPEFSFVE"
     gene            1052..1954
                     /locus_tag="AC16HB_17705"
     CDS             1052..1954
                     /locus_tag="AC16HB_17705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR5608682.1"
                     /translation="MATVASMTGFANASGASSLGTLTAELKSVNARFLDLTLRVAEEL
                     RQFEGTVRETLSARIARGKVECRISVQRSGAAEAPRLNADALARLAQLAAEIAGRVPD
                     AAPLSVADALAWPGIVEVSSVDADALRVAMLALVSTALDQFEASRRREGEALAAGLLE
                     RCAAIEAIVAELRRRAPDMLAAIERKLVERLNQTLAPVLAGSGVVSREEISERIRQEV
                     TLYGLRADVDEELNRLATHVAEVRRVLAVGGAVGRRLDFLMQELNREANTLGSKATAI
                     ELTQAAVELKLLIEQMREQIQNLE"
     gene            1960..2601
                     /gene="gmk"
                     /locus_tag="AC16HB_17710"
     CDS             1960..2601
                     /gene="gmk"
                     /locus_tag="AC16HB_17710"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5608683.1"
                     /translation="MASGADERRPGNIFLVVAPSGAGKSTLVNALLARDSAIELSVSL
                     TTRPPRPGEQDGREYHFVSVEDFKARRARGEFLENAEVHGNFYATSRVWIDERVRAGR
                     DVLLEIDWQGARQVKQHFPDAVGVFILPPSIDALETRLKKRGQDSPQVIARRLLAAGS
                     EMAHAHEFDYVIINEDFELALAQLTAIVIATRLRYPTQASRHHDLFVQLGVLV"
     gene            2637..2840
                     /gene="rpoZ"
                     /locus_tag="AC16HB_17715"
     CDS             2637..2840
                     /gene="rpoZ"
                     /locus_tag="AC16HB_17715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR5608684.1"
                     /translation="MARITVEDCLKHIPNRFQLALAAAYRARQLTQGHAPKIDTKDKP
                     SVAALREIAAGRVGIEMLRKVPS"
     gene            2954..5287
                     /locus_tag="AC16HB_17720"
     CDS             2954..5287
                     /locus_tag="AC16HB_17720"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5608685.1"
                     /translation="MAASNPPTTGAPDLTAPAVPAAPSGFARRAAEALRKAVRRTPEP
                     PTPATAGVVSFTQLTEKLKKYLSGSDIQRIKEAFRFSDAAHLGQFRKSGLPYITHPIA
                     VAELLTEWKLDAAAIQAALLHDVLEDSGVTKQELVERFGAVVAELVDGVSKLDRLRFD
                     STEEQQAESFRKMLLAMARDVRVILIKLADRLHNVRTLDAMERDKQRRIALETLDIYA
                     PIAHRLGLNAIYRELQEGSFARLHPLRFQVLQRAVLAARGNRREVLGKILDAVRRALA
                     EHKIKAEVYGREKTIYGIYRKMVEKNLSFSEVLDIYGFRIVVNARQDCYLVLGALHAL
                     YRPVPGRFKDYIAIPKVNGYQSVHTTLIGPYGTPIEFQIRTREMNNVAESGVAAHWLY
                     KTDDTSLSELQSRTHQWLQSLLDIHQQTGDSAELMENIKVDLFPDKVYVFTPKGKIVS
                     LPRGATPVDFAYSIHTDIGNKCVAARINNEIQPLRTELRNGDVVEVVTGPVARPNPGW
                     LAFVRTGKARAEIRHFLRTMKREESVELGERLLTQAARQLDFALADAPATRWEALIKD
                     AQARDREEILADIGLGRRLAAVVARQLMLGLQPAAGQAVTETSRASFTTPAPVVIRGT
                     EGMALQMASCCCPIPGDTVVGHMRRDQGLAVHQADCPNARRARRADPERWIDLQWAEE
                     ITGLYGVNIDVSAANQRGVLGRIAIAIAEADSNILNVHLEDEGSDQALIHFKVQVRNR
                     KHLARLIRTLRRIKEVSRVVRARATVRASGPETADEA"
     gene            complement(5294..5839)
                     /gene="greB"
                     /locus_tag="AC16HB_17725"
     CDS             complement(5294..5839)
                     /gene="greB"
                     /locus_tag="AC16HB_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR5608686.1"
                     /translation="MSKAFVKESDAADDEDIEAPPLPAGSKNYITPAGYRRLKDELVQ
                     LLNSERPEVVRTVSWAASNGDRSENGDYIYGKKRLREIDRRVRFLTKRLDIAEIVDPT
                     ARPATDQIFFGATVRYATSGGDELQITIVGIDEVDPARGHVSWISPIARALLKAREGD
                     SVQLRTPAGIETLEILEVRYA"
     gene            complement(5859..5933)
                     /locus_tag="AC16HB_17730"
     tRNA            complement(5859..5933)
                     /locus_tag="AC16HB_17730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5899..5901),aa:His,seq:gtg)
     gene            complement(5944..6020)
                     /locus_tag="AC16HB_17735"
     tRNA            complement(5944..6020)
                     /locus_tag="AC16HB_17735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5984..5986),aa:Arg,seq:tct)
     gene            complement(6058..6134)
                     /locus_tag="AC16HB_17740"
     tRNA            complement(6058..6134)
                     /locus_tag="AC16HB_17740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6098..6100),aa:Pro,seq:tgg)
     gene            complement(6195..7538)
                     /gene="phoR"
                     /locus_tag="AC16HB_17745"
     CDS             complement(6195..7538)
                     /gene="phoR"
                     /locus_tag="AC16HB_17745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HIR5608687.1"
                     /translation="MNREQARQQFRIVVPALLRLALVGLVGAIVGVVFGAAAGLWTAL
                     ACVLALFGFHLFYLSALGHWVENPRLETIPGGIGIWSEVFTRLYKMRRAIESNERRLI
                     DSEERFRRTISALPEGIVLIDAALQIEWCNPVAERHLGISLRGDQGLRLTNLVRDPAF
                     VAFMTAGNFDDPLVFRPISNPALILALTVIEFEPARSIVISRDVTQSERVDAMRRDFV
                     ANVSHELRTPLTVVNGFLETLIDAQPQFDATRMRHLQLMQEQATRMSRLVEDLLMLSR
                     LESAETPLAEEQIDVPRLVRELEAEARALSQGRHLIGIEVTAARIRGSRDELRSAFGN
                     LVSNAIRYTPDGGRITLRWRVLGDGAQFEVEDTGIGIAPEDIPRLTERFYRVDKSRSR
                     ETGGTGLGLAIVKHVLLRHQGRLDVRSQIGHGSVFAAWLPSARVISADDNERKAA"
     gene            complement(7580..8275)
                     /gene="phoB"
                     /locus_tag="AC16HB_17750"
     CDS             complement(7580..8275)
                     /gene="phoB"
                     /locus_tag="AC16HB_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018573660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR5608688.1"
                     /translation="MPASILVVEDEPAIQELVSYTCSSHGYQVRRVDSAGSAQAAIRD
                     RLPDLVILDWMLPDRTGIELLRELRAHERTRALPVIMLTAKGAEGDRVAGLDAGADDY
                     VVKPFSPRELVSRVRAVFRRRAPDKSGESLSYGPVTIDPARHEVTVDGKPVRIGLAEF
                     KLLKFMMSQPDRVFTRGQLLDSVWGDHVFVEERTVDVHVLRLRKALAAANAQHLVQTV
                     RGLGYRLSTRVEQ"
     gene            complement(8284..8997)
                     /gene="phoU"
                     /locus_tag="AC16HB_17755"
     CDS             complement(8284..8997)
                     /gene="phoU"
                     /locus_tag="AC16HB_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR5608689.1"
                     /translation="MATGEHSSRQYDHDLETIRSHVLQMGGLVESQLKTAIDAFERQS
                     MEQADDAIHADDRVDEMQLEIDRMVNNVIARRQPTAGDLRLIMGVAKTLTDLERTGDE
                     ASKIARAAKWLQEKGNISRLNRIPGIRVSGDTAARMLRGALDAFARLDAVAAAAIIRE
                     DQGVDEHFRAILRQLITFMMEDPRLISAAIDTIWVAKAIERVGDHAKNIAEHVIFIAQ
                     GADVRHAPPEVVAQAVRKI"
     gene            complement(9014..9790)
                     /gene="pstB"
                     /locus_tag="AC16HB_17760"
     CDS             complement(9014..9790)
                     /gene="pstB"
                     /locus_tag="AC16HB_17760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5608690.1"
                     /translation="MSAAAASVPSKMTVRNLNFYYGRFQALKNINLDIAEKKVTAFIG
                     PSGCGKSTLLRVFNRMFELYPEQRAEGEVVVDGENVLTTAEDVALVRAKIGMVFQKPT
                     PFPMSIHDNIAFGVRLFESLSRPEMEERVEWALRKAALWNEVKDKLAQSGTSLSGGQQ
                     QRLCIARGIAIRPEIILFDEPCSALDPISTAKIEELIEELKQDYTVAIVTHNMQQAAR
                     VSDYTAYMYLGELIEFGETEQIFIKPKKRETEDYITGRFG"
     gene            complement(9801..10676)
                     /gene="pstA"
                     /locus_tag="AC16HB_17765"
     CDS             complement(9801..10676)
                     /gene="pstA"
                     /locus_tag="AC16HB_17765"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5608691.1"
                     /translation="MSVVTLSRPVDHRLPLHRRRKLVNAFALTLSLAAMAFGLFWLIW
                     ILFETIALGIGGLSLQLFVASTPAPNVEGGGLANAIVGSVLMVALATLLGTPIGIFAG
                     IYLAEYGRRGWLGAATRFINDILLSAPSIVIGLFIYAVVVAQMRKFSGYAGVLALALI
                     VIPVVVRTTENMLQLIPNALREAAFALGAPKWVVIRSITLRAARAGVVTGVLLALARI
                     AGETAPLLFTALSNQFFSVDLAAPMANLPVTIYKFAMSPFANWQQLAWAGVFLITLGV
                     LLLNVLARVLFRGKP"
     gene            complement(10673..11638)
                     /gene="pstC"
                     /locus_tag="AC16HB_17770"
     CDS             complement(10673..11638)
                     /gene="pstC"
                     /locus_tag="AC16HB_17770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HIR5608692.1"
                     /translation="MKLEEPPTQRRVSPVGDLVFAGLARGAAILTLALLAGIIVSLII
                     GAWPALRAYGLEFLWTAEWDPVQERFGGLVMIYGTLMTSFIALVIAVPVSFGIALFLT
                     ELSPRWLKRPLGTAIELLAAIPSIVYGMWGLLVFAPILSQYVQQPLQRAFAGVPLLGT
                     LVSGAPVGIGILSAGIILAIMIIPFIAAVMRDVFEVTPPMLKESAYGLGATTWEVVWR
                     VVLPYTKVGVVGGIMLGLGRALGETMAVTFVIGNMNQLSSLSLFEAANSITSALANEF
                     AEAAAGKHQASLIYLGLVLFLITSIVLAASKVLLARMQRGEGARA"
     gene            complement(11722..12753)
                     /gene="pstS"
                     /locus_tag="AC16HB_17775"
     CDS             complement(11722..12753)
                     /gene="pstS"
                     /locus_tag="AC16HB_17775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIR5608693.1"
                     /translation="MRILAKLSIAALAAGATLPLAAQEITGAGATFPAPLYARWAADY
                     NKATAARINYQSVGSGAGLQQIRAKTVDFGASDMPLADDVLKKDGLIQFPTVIGGVVP
                     VVNIPGVQAGQMKLTGPVLADIYLGKIGKWNDAAIAKLNPGLKLPDAPIAVVRRADGS
                     GTTFIFTNYLSKVSAEWKAKVGEGTAVNWPSGAGGKGNEGVAAFVQRLPNSIGYVEYA
                     YAKQNKMSHALLQNAAGSFVAPDDATFAAAAAGAEWSKSFYQILTNQPGKDAWPVSGA
                     TFIMMHAVQDKPQQAAAALRFFDWAYKNGDKTASELDYVPLPAVVKDMIRKHWSQIRD
                     AGGQAIVGG"
     gene            complement(12975..14309)
                     /gene="glmM"
                     /locus_tag="AC16HB_17780"
     CDS             complement(12975..14309)
                     /gene="glmM"
                     /locus_tag="AC16HB_17780"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5608694.1"
                     /translation="MSRKYFGTDGVRGTVGQMPITPGFVMRLGQAAGHVLARIAGQRP
                     TVLIGKDTRISGYMLEAALEAGFSSVGVDVVLCGPLPTPAVAYLTRALRLTAGVVISA
                     SHNPYPDNGIKFFSAQGKKLPDDTEQAIEEEMDRTQECVESRLLGKARRLDDAAGRYV
                     EFCKSTFPNEFDLKGLKIIVDCAHGAAYHVAPDVFHELGAEVVAVGAEPNGFNINDGV
                     GAVHATHLSEVVRAHGADFGIALDGDADRVVIADGSGRVYNGDELLYVIVRDRLAING
                     VRGAVGTLMTNFALERRLGELRVPFERAKVGDRYVLELLEQRGWLLGGEGSGHILCLD
                     KHTTGDGIISSLQVLAAMRRARQTLAQLTADLPLLPQALVNVRIANQFDWKQHAPLAR
                     EIELVEKQLYGSGRVLIRASGTEPVLRVMVEAGDDALAESLARRLAATVTGA"
     gene            complement(14306..15094)
                     /gene="folP"
                     /locus_tag="AC16HB_17785"
     CDS             complement(14306..15094)
                     /gene="folP"
                     /locus_tag="AC16HB_17785"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5608695.1"
                     /translation="MGVINVTPDSFSDGGRNLDPVAARDTAQRLIEEGADLLDIGGES
                     TRPGAAAVGVEEELQRVLPLVRALREAGVPISVDTSKPEVMHAVLAEGASIINDVRAL
                     QAPGAVGVAARTDCGMVLMHMQGSPRTMQAAPHYEDVVREVVEFLRARRDALVQAGVG
                     AGRIALDPGFGFGKTVAHNLALLARLDALARLGQPVLAGLSRKSMLGQLTGRPVDQRL
                     HASVAAALLAVQRGANIVRVHDVAATRDALRVWAALRAFEEGTT"
     gene            complement(15204..17105)
                     /gene="ftsH"
                     /locus_tag="AC16HB_17790"
     CDS             complement(15204..17105)
                     /gene="ftsH"
                     /locus_tag="AC16HB_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR5608696.1"
                     /translation="MNNNMFAKAAIWMVIALVLFTVFKQFEGRQRPGESYLPYSDFLA
                     EVKSGRVKSVTIQESGVTPEVVAVTNDDRRIRTVATVLDRGLMGDLINAGVKVDVKPR
                     EEQSLLTSIFVSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESNNTITF
                     VDVAGCDEAKEEVQELVDFLRDPTKFQKLGGRIPRGVLMVGSPGTGKTLLAKAIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFENAKKHAPCIIFIDEIDAVGRQRGAGLGGG
                     NDEREQTLNQLLVEMDGFETGQGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIR
                     GREQILNVHMRKVPIGADVKADVIARGTPGFSGADLANLVNEAALFAARRSARVVEMQ
                     DFERAKDKIMMGAERKSMVMSEEERRNTAYHESGHAVVAKLVPKSDPVHKVTIIPRGR
                     ALGVTMQLPEQDRYAYDRVYLLSRIAVLFGGRIAEEVFMSQMTTGAGNDFERATQMAR
                     DMVTRYGMSDVLGPMVYAENEGEVFLGRSITKTTHVSEETMRKVDAEIRRIIDEQYAL
                     ARKLIEDNRDKVEAMAKALLELETIDADQIDDIMAGKPPRPPKPMSSTSTTGGKSESG
                     GAEGAAQPA"
     gene            complement(17244..17930)
                     /locus_tag="AC16HB_17795"
     CDS             complement(17244..17930)
                     /locus_tag="AC16HB_17795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIR5608697.1"
                     /translation="MPVAKKKHRSNPGWIRRHVTDPYVKAATRDGYRSRAAYKLIEID
                     AKDKLLRPGMTVIDLGAAPGSWAQVVRERLMTKYGVRGRIVALDILPMDPLPDVQAIQ
                     GDFREEPVAAELAAALAGQKADVVLSDLAPNLSGIAAADAARSLHLAELALEFAQAHL
                     KPAGVLLVKAFQGSGYSQFVEQLKRSFVSVAARKPPASRTESAETYLLARGLKVRVNP
                     ETEQTAEGLK"
     gene            17948..18361
                     /gene="yhbY"
                     /locus_tag="AC16HB_17800"
     CDS             17948..18361
                     /gene="yhbY"
                     /locus_tag="AC16HB_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR5608698.1"
                     /translation="MAELNLTRDDRLALKARAHHLNPVVLLGASGLSDAVVREIDRAL
                     NAHELIKIRVPGEDRDERERLFADLAERLGAARVQAIGKLLVLYRPKPAEVAPAKPVP
                     PRRTERASAARAKPRRDEIRRTQRRVAAPRGRRQA"
     gene            complement(18352..18825)
                     /gene="greA"
                     /locus_tag="AC16HB_17805"
     CDS             complement(18352..18825)
                     /gene="greA"
                     /locus_tag="AC16HB_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR5608699.1"
                     /translation="MPIPITVRGAERLKEELQRLKAVDRPAIIQAIKEAREKGDLSEN
                     AEYEAARERQGFIEGRINELEGKVGSLQIIDPATVDAGGRVVFGATVELEDLTAGESV
                     KYQIVGDDEADIKAGLISISSPIARALIGKVEGDVIEVAAPGGRREYEILMVSYA"
     gene            19056..20051
                     /locus_tag="AC16HB_17810"
     CDS             19056..20051
                     /locus_tag="AC16HB_17810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5608700.1"
                     /translation="MIPFSILDLAPIVVGGDAAQALANTLDLARHAERWGYRRYWLAE
                     HHNMDGVASSATAVLVGQVATVTQSIRVGSGGVMLPNHSPLVIAEQFGTLATLFPDRI
                     DLGIGRAPGTDPLTMRALRRHLAQEDDDFIADVRELQSYLGPPVPAARVRAIPGVGTN
                     VPIWLLGSSLYGAQAAALLGLPFAFASHFAPALLPQALAIYRERFRPSATLERPYAMV
                     GVNVIAADTDTSAARLFTSAQQRFLGMTRGLRGPLPRPVPTMDGLWNEDERAQVEQML
                     AVSAVGSPATIEHRLAELVEATGADELIVAAAIHDHAARLRSYELLAQIGCALRQ"
     gene            complement(20040..23246)
                     /gene="carB"
                     /locus_tag="AC16HB_17815"
     CDS             complement(20040..23246)
                     /gene="carB"
                     /locus_tag="AC16HB_17815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5608701.1"
                     /translation="MPKRTDIQSILIIGAGPIVIGQACEFDYSGAQACKALKQEGYKV
                     ILVNSNPATIMTDPETADVTYIEPITWEVVERIIAKEKPDAVLPTMGGQTALNCAMDL
                     YKHGVLHKHHVELIGASPDAIEKAEDRQKFKEAMTRIGLASARSGIAHSMDEALAVQK
                     AMGLPVVIRPSFTLGGSGGGVAWNMEEFTAICTRGLDLSPTRELLIEESLLGWKEFEM
                     EVVRDKADNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQLMRNASIAILREIG
                     VDTGGSNVQFAIDPGTGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNEITGGATPASFEPTIDYVVTKVPRFAFEKFRAADPHLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGADGLDEHSTNRDEIVEEIGDPGPERIWFVADAFRIGMSLAEVREE
                     TAIDPWFLAQIEELVAIEARLKGRSLGSLTAEEMRFLKRKGFSDRRLARLLGTKADDV
                     RGARRVLGVRPVYKRVDTCAAEFATTTAYMYSTYEDECEANPTSKKKVIVLGGGPNRI
                     GQGIEFDYCCVPAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIV
                     DKEKPFGVIVQYGGQTPLKLARDLEANGVPIIGTTPDSIDIAEDRERFQQLLHKLELK
                     QPPNRTARTEDEALKLAQEIGYPLVVRPSYVLGGRAMEIVQDDKDLERYMREAVKVSN
                     DSPVLLDRFLDDAIEVDVDCVADGREVRIGGVMEHIEQAGVHSGDSACSLPPYSLSAA
                     MIEELKRQTTLMAERLNVVGLMNVQFAIQDGTVYVLEVNPRASRTVPFVSKATGEPLA
                     KIAARCMVGRSIAAQGARVEVVPPYFSVKEAVFPFAKFPGVDPLLGPEMRSTGEVMGV
                     GRNFGEALSKSQLGASARLPATGTVFLSVKDVDKPRTVEVARMLHEMGYQLVATRGTA
                     AAIEAAGIPVKPVNKVKDGRPHVVDLLKNGEIDLVITTVSENRAQIADSRSIRTTALA
                     QRVNYYTTIAGGRAAVEGMKYLDRLEVYDLQALHRSLA"
     gene            complement(23323..24462)
                     /gene="carA"
                     /locus_tag="AC16HB_17820"
     CDS             complement(23323..24462)
                     /gene="carA"
                     /locus_tag="AC16HB_17820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR5608702.1"
                     /translation="MSSLISDSRPAALLALADGTIFRGRSIGAAGHCVGEVVFNTAIT
                     GYQEILTDPSYCRQIVTLTYPHIGNTGTNAEDVESVKVFAAGLVIRDLPPLHSSFRAQ
                     ASLTEYLVRENLVAIADIDTRKLTRALRDKGAQPGAILVGTDETRAVALARSFPGMAG
                     LDLAKVVSADAPYEWREAAWSLDAGYGKLTEPKRHVVAYDYGVKRNILRLLAQRGCRV
                     TVVPAQTPAAEVIRLEPDGVFLSNGPGDPEPCDYAIEAARELIAKGYPTFGICLGHQI
                     MGLASGGRTLKMKFGHHGANHPVQDLDDRRVLITSQNHGFAVDPKTLPDTCRVTHVSL
                     FDGTLQGFRRTDKPAFCFQGHPEASPGPHDIVYLFDRFVKLMEER"
     gene            24589..26115
                     /locus_tag="AC16HB_17825"
     CDS             24589..26115
                     /locus_tag="AC16HB_17825"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5608703.1"
                     /translation="MLILPYQTRFALRSTPVVTLALILINALVFFVAQARDDRVYEEA
                     YRFYDRSALPRIELSHYRDWLARQAKDPDRRDQLADLQESLREQDFEGALALMQSDDD
                     FMRSLRQLNVVTPGDPEFLTWRDQRNQYEALLHSSATERFLLKREQLQPWRFVTYQFL
                     HGDLGHWLGNMIVLLLAGSFAEAVLGRWRFLFAYLLSGVGAGALQVALSDLPVIGASG
                     AIAGAMAMVAVLYGLRRVPVFVSVLFYFDTFRMPALWLLPIWIANELWQWANAAEESV
                     AYAAHLGGFAIGALLAWMLRPARSLEPASEPEADSGGDDARLRLQQRAEQAAAAVDIP
                     RATHLYGELAAQYPMRSDYAVASYNIALLGADRNALRNAAEHVLVNRPGRANAELRRA
                     YLHMAQSKSRDLLPVEAQLRLARRLVRAREDGAALQLIDALLADDKRRTEHARALSDC
                     LLGLHTTYLRYGLTPQADDVRRRLAQYFTPPGAGAAPMPAPSGHTTLQGPSTLNFDLP
                     "
     gene            complement(26125..27378)
                     /locus_tag="AC16HB_17830"
     CDS             complement(26125..27378)
                     /locus_tag="AC16HB_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608704.1"
                     /translation="MSTSFSPDAPSPVPRVPATPFWRRMPQFFRFPAYRAPLLRNLAL
                     SALLAGALALQSRWLASLVASGASIYLAYYGFLVIERVAQGYLHPDRFPAARPIHPWR
                     PLKLAAIMLTAFIGIALVTSVTGDGVLTLVADIAVALLLPACIMTLAVTDSLRQAINP
                     RRVFDLARRIGAPYLVLFLFLLLLMGGSQQAFELVAPALGESAWLLALASSFVGNYFF
                     FIMCALMGYVLYQYSDVLGLQVTGPGEEAAAEAATAGWRDTALARLMAAGDIGSAIEL
                     LERESIARPDDTAVHERLRRLLALAGDRARRDTHLERYLEVLLQRQPGQALALARDVL
                     AGDPDWTPASGALAVRLARAALDARQFDLVLALTREFESRHGGDDSAPTVVLMRAQAL
                     AESGQAEAAEREMRDLAATAAARRD"
     gene            complement(27405..28232)
                     /locus_tag="AC16HB_17835"
     CDS             complement(27405..28232)
                     /locus_tag="AC16HB_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608705.1"
                     /translation="MWSIIKPNPRNLRVWQVGVAVLLFVFWHMMTRPGLIPPFMFEND
                     RQAAFFFGEPLAIFGRIWNWFFAQRDIYEHLWVTLLETVLAFAIGTSAGLAMGMWLAL
                     SPMAAGILDPYIKAANSMPRVILAPIFAVWFGLGVASKVALGFTLVFFIVFFNVYQGV
                     REVSPTVLANARMLGANRRQLLRHVYLPSAMSWVFSSLHTSVGLAFVGAVVGEYLGSA
                     RGVGYLILQAEGTFDINTVMAGILVLTGFALVLDWVVGVGEKRLMRWQPRSGETEKL"
     gene            complement(28246..28791)
                     /locus_tag="AC16HB_17840"
     CDS             complement(28246..28791)
                     /locus_tag="AC16HB_17840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5608706.1"
                     /translation="MMKQALVIIDIQNDYFPGGRMPLDGPAAAAGRAAHVLQWQRERG
                     LPVFHVQHVSTRAGATFFLPDTEGVQIHTAVAPHPGESLIVKHFPNAFRETSLAGQLR
                     AAGVDELLFVGMMTHMCIDTSVRAAADLGFRCSVAADACATRDLQWGGQTVPAPQVHA
                     AFLAALNGAFARVATVEELLA"
     gene            complement(28788..29594)
                     /locus_tag="AC16HB_17845"
     CDS             complement(28788..29594)
                     /locus_tag="AC16HB_17845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608707.1"
                     /translation="MNALSLDRITCTFISRDAPGQRYTAVREVTLSVGAGEFVSVVGP
                     TGCGKSTLLNVAAGLLEPSSGAVELFGAPLAGINARAGYMFQSEALMPWRTTLANVSA
                     GLEFRGVPSAEARAQADAWLKRVGLGGFGDRYPHQLSGGMRKRTSLAQTLVLDPDIIL
                     MDEPFSALDIQTRQLMENEVLDLWAAKKKAVLFITHDLDEAIAMSDRVIVLSAGPASH
                     PIGEFAIDLPRPRDVAEVRVTPRFIELHKAIWGVLREEVLKGYEQQKRAA"
     gene            complement(29676..30701)
                     /locus_tag="AC16HB_17850"
     CDS             complement(29676..30701)
                     /locus_tag="AC16HB_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608708.1"
                     /translation="MQTTRRNLLLAGAAAIAAPAFVRAQALEKKKVTIAVGGKNLLYY
                     LPLTVAEQLKYFDAEGLEISIVDFAGGAQALRAVVGGSADVVSGAFEHTLNMQAKGQP
                     MRAFVLQGRAPQIVLGVNSKTMPNFKSIADLKGKKIGVTAPGSSTNVMANFVLAKAGL
                     KPTDVSIIGVGASQGAVAAIRSGQIDAISNLDPVITILARSGDIKIVSDTRDVAEADK
                     VFGGPMPAACLYAPVSFLEKNPNTAQALVNAIVRADQWIQKAGPSDIIKVVPESYLLG
                     DRAVYIDAFLKAKGALSPDGMIPDAGSATALRALASVDESMKTAKLDLKANYTNEFVK
                     KALAKYK"
     gene            complement(30718..31917)
                     /locus_tag="AC16HB_17855"
     CDS             complement(30718..31917)
                     /locus_tag="AC16HB_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5608709.1"
                     /translation="MSALRGVKVLELGTLIAGPFAARMLAEFGAEVIKIEATDGGDPI
                     RTWRHLHGGTSLWWYVQARNKKSVTLNLKDRRAQDIARKLALDADVVIENYRPGVLEK
                     WGLGYEHLRALNPATIMVRLSGYGQTGPMRDKPGFGAIGESMGGIRYVSGFPDRPPMR
                     IGISIGDSIAALHGVIGALMALLHRDATGGRGKGEGQLVDVALYEAVFNLMESTVPEY
                     DRAGVIRQRTGGALPGIVPSNTYTCADGAAIVIAGNGDAIFKRLMTAIGRADLANDPA
                     LARNDGRVPRTQEIDAAIQAWCSGRNIDAAVAALTAADVPVSKIYSVADMFRDAQFAA
                     RAMIERHRLPDGTDLAVPAVTPKLSATPGETRWLGPRLGEHTDQVLAALGYDESELAA
                     LRKDGVI"
     gene            complement(31914..32297)
                     /locus_tag="AC16HB_17860"
     CDS             complement(31914..32297)
                     /locus_tag="AC16HB_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR5608710.1"
                     /translation="MTVALWCVLIAGILPYLATTIAKAGGERFNNRDPRAWLAKQQGF
                     RARAHAAQLNSFEAFPFFAAAVIVAHLTQASAERISALAVIFIVVRLGYLASYLADWH
                     WARSVLWLVGWLATITIFVSGLGAA"
     gene            complement(32308..32871)
                     /gene="recR"
                     /locus_tag="AC16HB_17865"
     CDS             complement(32308..32871)
                     /gene="recR"
                     /locus_tag="AC16HB_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5608711.1"
                     /translation="MKRLPGIGPRSAQRIAFHLLQHDRDTAVRLGEALLAAHANVRHC
                     ARCNTLTEQEICETCSNPKRDAGLLCVVESPSDQLMLEQSLSYAGLYFVLLGRLSPLD
                     GVGPREIQMERLLARACDGLVREVIVATSFTPEGDATAHYIAELLRARDPQLKVTRLA
                     RGVPAGAELEYTDVNTLAQAMLDRRAA"
     gene            32758..>32990
                     /locus_tag="AC16HB_17870"
     CDS             32758..>32990
                     /locus_tag="AC16HB_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608712.1"
                     /translation="MGRKQRLAQSNGGVAIVLQQMKRDPLRRPRSDAGQTFQRVDQFV
                     EGGPEFHTQNGSLNPGGIGMPAVIEAIFCWVVSS"
BASE COUNT         5426 a        11409 c        10728 g         5427 t
ORIGIN      
        1 gctcgacgaa cgagaactcg ggtcgctcgg gcacgttgtt gatcagctcc ttctgcaccg
       61 cgtagatggt ctgcggccgc tcgagcgagt tcagccgcag gcagcgctcg accatcgaca
      121 gcagctccga cgagtacagc ccgcggaacg cgcgcagcac ctgcggcatc ttgtcctcct
      181 tcagccgcgc gtcggactcc tgcggcggca tgccggccat gcacgcgtag atgcaggcac
      241 cgatgctgta cacgtcggtc cacggtccga gctcggcatc gcgcttgtac agctccggcg
      301 cggcgaagcc cggcgtgtac atcggatagg ttttgctgat gtcccgctgc agcgtctgcc
      361 gcgcggcgcc gaagtcgagc aggatcggcg tgccgtccat gcgcagatag atgttcgccg
      421 gcttcacgtc gaggtgcagc aggcggttgg aatgcacttc gcgcagcccg ttcatcacct
      481 gcgtgaacac gcggcggatg aagcgctccg acagcacgtc cttctggtcc ttgctgcggt
      541 tgcgcagcac atgctcctgc agcgagcggc cggactcgta atgcatcacc atgtacaccg
      601 tgtcgttggc gcggaagaag ttcagcacgc gcacgatgtt cgggtggtag atcgacgcca
      661 gcgcgcggcc ttcctcgaag aagcacttga ggccgatgcg gtaggtgttc aggttctccg
      721 gcgccacgta cggtgccagt tcccccggac cccgcttggc gagggcagcc ggcatgtact
      781 ccttgatggc gaccgaattg ccgtgctcgt cggtggcgag atagacgatc gagaagccgc
      841 cgctggatat cttctttaca atgcgatatc cgccgacctc gatgccttcg ggaagtgctg
      901 cgttgttctg cgccgccatg gaggaagagt tcgcgagccg cggaatgcgt tgcccgcctg
      961 tgcggcgcgg ccgtcggtgt tccggaaagt ccggcgcgat tctagccgca gggcgcgtcg
     1021 cccggcggat tacccgagag gagttggacg catggcaacc gtcgccagca tgaccggatt
     1081 tgcgaatgcg agcggtgcca gttcattggg cacgctgacg gcggaactga agtcggtcaa
     1141 cgcccgcttt ctcgatctga cgctgcgcgt ggccgaggaa ctgcgccagt tcgaagggac
     1201 ggtgcgcgaa acactgagcg cgcgcatcgc gcgcggcaag gtcgagtgcc gcatcagcgt
     1261 gcagcgaagc ggcgcggccg aggcgccgcg gcttaatgcg gacgcattag cgcgcctcgc
     1321 gcaactggcc gccgagatcg ccggccgcgt cccggacgcg gcgccgctgt cggtcgccga
     1381 tgcgctcgcc tggcccggca tcgtcgaggt ttcgtcggtc gacgccgatg cactgcgggt
     1441 cgcgatgctt gcgctggttt cgaccgcgct cgaccagttc gaggcgagcc gccgccgcga
     1501 gggcgaggca ctggccgccg gactgctcga acgctgcgcc gcgatcgagg cgatcgtcgc
     1561 cgaactgcgc cgtcgcgcgc ccgacatgct ggccgcgatc gagcgcaagc tggtggagcg
     1621 cctgaaccag acgctggcgc cggtactggc cggcagcggc gtcgtatcgc gcgaggagat
     1681 ctccgaacgc atccggcagg aggtgacgct gtatggcttg cgcgccgacg tcgacgagga
     1741 gctgaaccgg ctcgccacgc acgtggccga ggtgcggcgt gtactggcgg ttggcggcgc
     1801 ggtcggacgc cggctggatt tcctgatgca ggagctgaac cgcgaggcca acacgctggg
     1861 ctcgaaggcc accgcgatcg aactgacgca ggcagccgtc gagctgaagc tgctgatcga
     1921 gcagatgcgc gaacagatcc agaacctgga gtgatcatga tggcgagcgg cgcagacgaa
     1981 cgcaggcccg gcaacatctt cctcgtggtg gcgccgtccg gcgcgggcaa gtcgacgctg
     2041 gtcaacgcgc tgctcgcgcg cgacagcgcg atcgagctgt cggtatcgtt gacgacgcgg
     2101 ccaccgcggc cgggcgagca ggacggacgc gagtatcact tcgtgtcggt cgaggacttc
     2161 aaggcgcggc gcgcgcgcgg cgagttcctc gagaacgccg aggtgcacgg caacttctac
     2221 gccacctccc gcgtgtggat cgacgagcgc gtgcgcgccg gtcgcgacgt gctgctggaa
     2281 atcgactggc agggcgcccg ccaggtcaag cagcacttcc cggatgcggt cggcgtcttc
     2341 atcctgccgc catcgatcga cgcgctggaa acgcggctga agaagcgcgg ccaggactcg
     2401 ccgcaggtga tcgcgcgccg gctgctcgcg gccggcagcg agatggcgca cgcgcatgaa
     2461 ttcgactacg tgatcatcaa cgaggacttc gaactcgcgc tcgcgcagct gacggcgatc
     2521 gtcatcgcga cacggctgcg ctacccaacg caggcgtcgc gtcaccacga cctgttcgta
     2581 cagcttggcg tcctcgtcta gtaaaatccg tccaatcccc gcaaaggaac ccggccatgg
     2641 cccgcatcac cgtcgaagac tgcctgaagc acattccgaa tcgcttccag ctcgcgctcg
     2701 ccgccgccta ccgcgcgcgc cagctgacgc aggggcacgc gcccaagatc gacaccaagg
     2761 acaagccgtc ggtggccgcg ctgcgcgaga ttgccgccgg acgtgtcggc atcgagatgc
     2821 tgcgcaaggt gccgtcgtga tgtgacgccc catgcgaccg cgacgcccca gcgcgtcttc
     2881 cggccgcagc ggcgctgacg cgcccgtttg cgccgccttg tgcctcacgc taagttagcg
     2941 ccgaggttcg cacatggcgg catccaaccc acccacgacc ggcgcgcccg atctgacggc
     3001 gcccgccgtt cccgccgcgc cgtccggttt cgcgcgccgc gccgcggaag cgctgcgcaa
     3061 ggcggtccgc cgcacacctg agccgccgac ccccgcgacc gccggcgtcg tcagcttcac
     3121 gcagttgacg gagaagctga agaagtacct ctcgggcagc gacatccagc gcatcaagga
     3181 agcattccgc ttctccgatg cggcacacct ggggcagttc cgcaagagcg gcctgccgta
     3241 catcacgcac ccgatcgcgg tcgccgagct tctgaccgaa tggaagctcg acgccgctgc
     3301 gatccaggcc gcgctgctgc acgacgtgct ggaggattcg ggcgtcacca agcaggaact
     3361 ggtcgagcgc ttcggcgccg tggtcgcgga actggtcgac ggcgtctcca agctcgaccg
     3421 attgcgcttc gactccaccg aggagcagca ggccgagagc ttccgcaaga tgctgctggc
     3481 gatggcgcgc gacgtgcgcg tgatcctgat caagctcgcc gaccgcctgc acaacgtgcg
     3541 cacgctcgac gcaatggagc gcgacaagca gcgccgcatc gcgctggaga cgctcgacat
     3601 ctacgcgccg atcgcgcatc ggttggggct gaacgcgatc tatcgcgaac tgcaggaggg
     3661 cagcttcgcg cggctgcatc cgctgcgctt ccaggtgctg cagagagccg tgctggcggc
     3721 gcgcggcaac cggcgcgaag tgctgggcaa gatcctcgac gcggtccgcc gtgcgctcgc
     3781 cgagcacaag atcaaggccg aggtgtacgg tcgcgagaag accatctacg gcatctaccg
     3841 caagatggtc gagaagaacc tgtcgttctc cgaggtgctc gacatctacg gcttccgcat
     3901 cgtcgtcaac gcgcgccagg actgctacct cgtgctcggt gcgctgcacg cgctgtaccg
     3961 gccggtgccg ggccgcttca aggactacat cgcgattccc aaggtcaacg gctatcagtc
     4021 cgtccacacg acgctgatcg gtccgtacgg cacgccgatc gagttccaga tccgcacgcg
     4081 cgagatgaac aacgtcgccg agtccggcgt ggcggcgcac tggctgtaca agaccgacga
     4141 cacgtcgctg tccgaactgc agtcgcgcac ccaccagtgg ctgcagtcgc tgctcgacat
     4201 ccaccagcag acgggcgact cggccgagct gatggagaac atcaaggtcg acctgttccc
     4261 ggacaaggtc tacgtgttca cgccgaaggg caagatcgtg tcgctgccgc gcggcgccac
     4321 accggtcgac ttcgcctaca gcatccatac cgacatcggc aacaagtgcg tggccgcgcg
     4381 catcaacaat gaaatccagc cgctgcgcac cgagctgcgc aatggcgacg tggtcgaggt
     4441 cgtcaccggc ccggtcgcgc ggccgaatcc gggctggctc gcgttcgtgc gcaccggcaa
     4501 ggcgcgggca gagatccgcc acttcctgcg cacgatgaag cgcgaggaat cggtcgagct
     4561 gggcgagcgc ctgctgacgc aggcggcgcg ccagctcgac ttcgcgctcg ccgacgcgcc
     4621 ggccacgcgc tgggaggcgc tcatcaagga cgcgcaggcg cgcgaccgcg aagagatcct
     4681 cgccgacatc ggtctcggcc ggcgcctggc ggcagtcgtc gcgcgccagc tgatgctcgg
     4741 gctgcaaccc gccgccgggc aggcggtcac ggagacttcg cgcgcctcgt tcacgacacc
     4801 ggcgccggtc gtgattcgcg gcaccgaggg catggcactg cagatggcga gctgctgctg
     4861 cccgatcccg ggcgacacgg tcgtgggcca catgcgcagg gaccaggggc tggccgtgca
     4921 tcaggccgac tgcccgaacg cgcgccgtgc gcgccgcgcc gatccggagc gctggatcga
     4981 cctgcaatgg gccgaggaaa tcaccggcct gtacggcgtc aacatcgacg tcagcgccgc
     5041 caatcagcgc ggcgtgctgg gccggatcgc gatcgcgatt gcggaggccg actcgaacat
     5101 cctcaacgtg cacctggagg acgaaggttc ggaccaggcg ctgatccatt tcaaggtgca
     5161 ggtgcgcaac cgcaagcatc tggcgcggct gatccgcacg ctgcggcgga tcaaggaagt
     5221 ctcccgcgtc gtgcgcgccc gcgccaccgt gcgcgcgagc ggaccggaaa ccgccgacga
     5281 agcctgatcg cgctcaggcg tagcggactt cgaggatctc cagcgtttcg attccggccg
     5341 gcgtgcgcaa ctgcacgctg tcgccttcgc gcgccttcag cagcgcacgc gcgatcggcg
     5401 agatccagct cacgtgcccg cgcgcaggat cgacctcgtc gatgccgacg atcgttatct
     5461 gcagttcgtc gccgcccgac gtcgcgtagc gcaccgtcgc gccgaagaag atctggtcgg
     5521 tggcgggccg cgcggtgggg tcgacgatct cggcaatgtc gagccgcttg gtcaggaagc
     5581 ggacgcggcg gtcgatctcg cgcaaccgct tcttgccata gatgtagtcg ccgttctccg
     5641 accggtcgcc gttgctcgcg gcccaggaca ccgtgcgcac gacctcgggc cgctcggagt
     5701 tcagcagttg gacgagttcg tccttcaggc gcctgtagcc ggccggcgtg atgtagttct
     5761 tcgatcccgc aggcagcggc ggcgcctcga tgtcctcatc gtcggcagcg tcggattcct
     5821 tgacgaacgc tttgctcatc agcgacgttg gagcacggtg gggtggccga tgggattcga
     5881 acccacaaca accggaatca caatccggga ctctaccgtt gagctacggc caccgctttg
     5941 ttctggcctg cccggcagga ctcgaacctg cgacccccgg cttagaaggc cggtgctcta
     6001 tccagctgag ctacgggcag ttcggttccc cgggcgagga gtgctgcaga aaaaatctgg
     6061 tcggggcgag aggattcgaa cctccgaccc cctgcgccca aggcaggtgc gctaccaggc
     6121 tgcgctacgc cccgaaggca tgcgcatgat accggcgcgc ggcaggtcgg gcaatttcgg
     6181 cgcgcgatgt tcgactacgc cgcctttcgc tcgttgtcgt ccgccgagat gacccgcgcg
     6241 ctcggaagcc atgccgcgaa cacgctgccg tgcccgatct gcgagcgaac atcgagccgg
     6301 ccctgatgcc gcagcagcac atgtttgacg atcgccagcc ccagaccggt gccacccgtt
     6361 tcgcgcgagc ggctcttgtc gacgcggtag aagcgctcgg tcaggcgcgg gatgtcttcg
     6421 ggcgcgatgc cgatgcccgt gtcctcgacc tcgaactgcg cgccgtcgcc caggacgcgc
     6481 cagcgcagcg tgatgcggcc gccatccggc gtgtagcgga tcgcgttgga aaccaggttg
     6541 ccgaacgcgc tgcgcagttc gtcacgactg ccacgaatcc gtgctgcggt gacctcgatg
     6601 ccgatcagat ggcgtccctg cgacagcgca cgcgcctccg cctccagttc gcgcaccagg
     6661 cgcggcacgt cgatctgttc ctcggcaagc ggtgtttcgg ccgattcgag ccgcgacagc
     6721 atcagcagat cttcgaccag ccgcgacatg cgcgtcgcct gctcctgcat cagctgcagg
     6781 tggcgcatgc gcgtggcatc gaactgcggc tgggcatcga tcagcgtctc gagaaagccg
     6841 ttgacgacgg tcagcggcgt acgtagttca tgcgacacgt tggcgacgaa atcccgccgc
     6901 atcgcgtcca cgcgttcgga ttgcgtcacg tcgcgcgaga tcacgatcga acgcgcaggt
     6961 tcgaactcga tcaccgtcag cgccaggatc agtgccgggt tactgatcgg ccggaacacg
     7021 agcggatcgt cgaaattgcc cgccgtcatg aacgcgacga acgccgggtc gcgcacgaga
     7081 ttcgtgagac gcagcccctg gtcgccgcgc aggctgatac cgaggtgcct ttccgccacg
     7141 gggttgcacc actcgatttg cagcgcggca tcgatcagca caatgccctc cggcagcgcg
     7201 ctgatcgtgc ggcgaaagcg ctcctcgcta tcgatcaggc gacgttcgtt cgattcgatc
     7261 gcgcgccgca tcttgtacag gcgggtgaag acctccgacc agatgccaat gccgcccgga
     7321 atcgtctcca gccgcgggtt ctccacccag tggccgagcg ccgacagata gaacaggtga
     7381 aatccgaaca gcgccagcac gcaggcgagc gcggtccaca gtcccgccgc tgcgccgaac
     7441 accacgccaa cgatcgcgcc gaccaatccg accagcgcca gccgcagcag cgccggaacg
     7501 acaatgcgga actgctgccg cgcctgttct ctgttcatcg cacctgtcgc gtcgtcctgc
     7561 ggcagcggtc gacggtcaat tactgctcga cgcgcgtcga caaccggtaa ccgagcccgc
     7621 gcaccgtctg caccaggtgc tgcgcgttcg cggccgccag tgccttgcgc agccgtagca
     7681 cgtgcacgtc caccgtgcgc tcctcgacga agacgtgatc gccccatacg ctgtcgagca
     7741 gttgtccgcg cgtgaacaca cggtccggtt gactcatcat gaacttcagc agcttgaact
     7801 cggccagccc tattctgacc ggcttgccgt cgaccgtgac ctcgtgccgg gccggatcga
     7861 tcgtcactgg gccgtagctg agcgactcgc cgctcttgtc gggcgcccgg cgccggaaca
     7921 ccgcgcgcac gcgcgacacc agctcgcgcg gagaaaaggg cttgaccacg taatcgtcgg
     7981 caccggcgtc cagtcccgcg acgcgatcgc cctccgcgcc cttcgcggtc agcatgatca
     8041 ccggcaacgc tcgcgtgcgc tcgtgcgcgc gcagttcgcg cagcagctcg atgccggtgc
     8101 ggtccgggag catccagtcg aggatcacga ggtcgggcag gcggtcgcgg atcgccgcct
     8161 gcgccgagcc ggccgaatcc acgcggcgca cctggtagcc atggctggag cacgtgtagg
     8221 agacaagctc ctggatcgcg ggctcgtctt cgaccaccag aatggacgcc ggcatctccc
     8281 gtctcagatc ttgcgcacgg cctgcgcgac cacttccggc ggcgcgtgac gcacgtccgc
     8341 cccctgcgcg atgaagatca cgtgctcggc gatgttcttc gcgtggtcgc cgacacgttc
     8401 gatcgccttg gcaacccaga tcgtgtcgat tgccgccgag atcaggcggg ggtcttccat
     8461 catgaacgtg atcagctggc gcaggatcgc gcgaaagtgc tcgtcgacac cctggtcctc
     8521 acgaatgatc gccgccgccg ccaccgcgtc cagccgggca aaggcgtcga gcgcgccgcg
     8581 cagcatccgg gccgcagtat cgccggatac gcgaataccc ggaatgcggt tcaggcgcga
     8641 gatgttgccc ttttcctgca gccatttggc ggcgcgcgcg atcttgctcg cctcgtcgcc
     8701 cgtgcgttcg agatcggtga gcgtcttggc aacgcccatg atgagcctca agtcgccggc
     8761 ggtgggctgg cggcgcgcga tcacgttgtt gaccatgcgg tcgatctcga gctgcatttc
     8821 gtcgacgcga tcgtcggcat ggatcgcgtc atcggcctgc tccatgctct gccgctcgaa
     8881 cgcgtcgatc gcggttttca gttgcgattc gaccagcccg cccatctgca gcacgtgcga
     8941 gcggatcgtt tccagatcgt ggtcgtactg gcgggacgaa tgctctccgg tggccatcgt
     9001 cgtcgtctcc tgctcagcca aaccggccgg ttatgtagtc ttccgtttcc cgcttcttcg
     9061 gcttgatgaa gatctgttcg gtctcgccga attcaatcag ttcgcccagg tacatgtacg
     9121 cggtgtagtc gctgacacgc gcggcctgct gcatgttgtg cgtgacgatc gcaaccgtgt
     9181 agtcctgctt cagttcctcg atcagttcct cgatcttggc ggtcgagatc gggtccagtg
     9241 ccgagcacgg ctcgtcgaac aggatgatct ccggacggat cgcgatgccg cgcgcgatgc
     9301 acaaccgctg ctgctgcccg cccgacaacg acgtaccgct ctgcgcgagc ttgtccttca
     9361 cctcgttcca gagcgcggcc ttgcgcagcg cccactcgac ccgctcctcc atctcgggcc
     9421 gcgacagaga ttcgaacagc cgcacgccaa acgcaatgtt gtcgtggatc gacatcggga
     9481 acggcgtcgg cttctggaac accatgccga tcttggcacg caccagagcc acgtcttcgg
     9541 cggtggtcag cacgttctca ccgtcgacga cgacctcgcc ctcggcgcgc tgctccggat
     9601 acagctcgaa catgcggttg aagaccctca gcagcgtcga cttgccgcag cccgacgggc
     9661 cgatgaacgc ggtgaccttc ttctcggcga tgtcgaggtt gatgttcttc agcgcctgga
     9721 acctgccgta gtagaagttc aggttgcgca cagtcatctt gctgggcacc gacgctgcag
     9781 ccgcggacat attcatcggt tcagggcttt cctcggaaca ggacgcgcgc caggacattc
     9841 agcagcagca cgccgagcgt gatcaggaac acgccggccc aggcgagttg ctgccagttg
     9901 gcgaacgggc tcatcgcaaa cttgtagatc gtgaccggca ggttggccat cggcgcggcg
     9961 agatcgacgc tgaagaactg gttcgacagc gcggtgaaga gcagcggcgc ggtttcgccg
    10021 gcaatgcggg caagcgccag cagcacgccg gtcacgacac cggcgcgcgc cgcgcgcagc
    10081 gtgatgctgc ggatcacgac ccacttcggt gcaccgaggg cgaacgcggc ttcccgcagc
    10141 gcattgggaa tcagttgcag catgttctct gtggtgcgca ccacgacggg gatcacgatc
    10201 agggccagcg ccagcacgcc cgcatagcca gagaacttgc gcatctgggc gaccacgacc
    10261 gcgtagataa agaggccgat cacgatcgac ggcgccgaca gcaggatgtc attgatgaag
    10321 cgcgtcgccg ccccgagcca gccgcgccgg ccatattcgg cgaggtagat gccggcaaag
    10381 attccgatcg gcgtgccaag cagcgtggcc agcgccacca tcagcaccga accgacgatc
    10441 gcgttggcca gcccgccgcc ctcgacgttc ggcgccggcg tggacgcgac gaacaactgc
    10501 agcgacaagc cgccgatgcc gagcgcgatc gtttcgaaga ggatccagat cagccagaac
    10561 aacccgaacg ccatcgccgc cagcgacagc gtgagcgcga acgcgttgac gagcttgcgc
    10621 cgccgatgca gtggcaagcg gtggtcgacc ggccgactca gggtgaccac gctcatgcgc
    10681 gcgccccttc gccgcgctgc atacgcgcga gcagcacctt ggacgccgcc agcacgatcg
    10741 aggtgatcag gaacagcacg agtccgaggt agatcagcga cgcctggtgc ttgcctgccg
    10801 ccgcctcggc aaattcgttg gcgagcgcgg aggtgatcga gttggccgcc tcgaacagcg
    10861 acaggctcga gagctgattc atgttgccga tcacgaacgt gaccgccatc gtctcaccga
    10921 gggcgcgccc cagcccgagc atgatgccgc cgaccacccc gaccttggtg tacggcagca
    10981 ccacacgcca caccacttcc caggtggtcg cgccgagccc gtaagcggac tccttcagca
    11041 tcggcggagt gacctcgaac acgtcgcgca tcactgcggc gatgaacggg atgatcatga
    11101 tcgccaggat gatgcccgcc gaaagaatgc cgatgccgac cggcgcgccg gaaacgagcg
    11161 tgcccagaag cggcacgccg gcaaatgccc tctgcagcgg ctgctgcacg tactgcgaca
    11221 ggatcggcgc gaatacgagc aggccccaca taccgtagac gatcgacggg atcgcggcca
    11281 gcagttcgat cgcggtgccg agcgggcgct tgagccagcg cggcgagagt tccgtgagga
    11341 acagcgcgat gccgaaactg accggcaccg cgatcacgag cgcgatgaac gaagtcatca
    11401 gcgtgccgta gatcatcacc agaccgccga accgctcctg caccggatcc cactcggcgg
    11461 tccataggaa ctccagcccg tacgcgcgga gcgcgggcca tgccccgatg atgagtgaga
    11521 cgatgatgcc ggcaagcagc gccagcgtga ggatggcagc gccacgggcc agcccggcga
    11581 agacgagatc cccgaccggg ctgacgcgcc tttgggtcgg aggctcttcc agcttcattg
    11641 cccgcgcatc gagttcaatc gatgcaggca gtgtagcgtc catgtccccc gtaccccgat
    11701 cgacgcttcg gtacgctcgc gtcagccgcc gacgatggct tgtccgccag cgtcgcggat
    11761 ctgcgaccag tgcttgcgga tcatgtcctt gaccacggcg ggcagcggca cgtagtcgag
    11821 ttcgctcgcg gtcttgtcgc cgttcttgta cgcccagtcg aagaagcgca gcgccgccgc
    11881 cgcctgctgc ggcttgtcct gcaccgcgtg catcatgatg aacgtggcac cgctgaccgg
    11941 ccacgcgtcc ttgcccggct ggttggtcag gatctggtaa aaggatttcg accattcggc
    12001 gccggccgca gccgctgcga acgtcgcatc gtcgggcgcg acgaaactgc cggccgcgtt
    12061 ctgcagcagc gcatggctca tcttgttctg cttcgcgtac gcatactcga cgtaaccaat
    12121 cgagttcggc agtcgttgca cgaaggctgc gacaccctcg ttgcccttgc cgcccgcgcc
    12181 gctcggccag ttcaccgcgg tgccctcgcc gactttcgcc ttccactccg cgctgacttt
    12241 cgacagatag ttggtaaaga tgaaggtcgt gcccgatccg tccgcgcgcc gcacgaccgc
    12301 gatcggtgcg tcgggcagct tcagaccggg attcagctta gcgatcgccg cgtcgttcca
    12361 cttgccgatc ttgccgagat agatatcggc cagcaccggc ccggtgagct tcatctgacc
    12421 cgcctgcact ccggggatgt tgacgaccgg caccacgccg ccgatcacgg tcggaaactg
    12481 gatcagaccg tccttcttga gcacgtcgtc ggcgagcggc atgtccgacg cgccgaaatc
    12541 gaccgtcttg gcgcggatct gctgcagccc ggcgcccgag ccgactgact gatagttgat
    12601 ccgcgccgcg gtggccttgt tgtagtccgc ggcccagcgt gcatacagcg gtgcgggaaa
    12661 ggtggcgccg gcgccggtga tttcctgtgc tgccagcggc agtgtggcac ccgccgccaa
    12721 cgcggcgatc gacagtttgg caagaattcg catggggtcg tccgatcagt tgtccaatga
    12781 cgcccgcagt ctagaaagcg aagatgacgc aaccgtgaca acgagcgcag ccgtgcggcc
    12841 ggtgccaggc aacgtgtcat cggaccctgt ggtccgtcat ccgactgtca tgtagcgccg
    12901 cggcggatcg tccgcccgct cctgcatgca gacctgcggc tttccggggt cgaagacggc
    12961 ctgcgccgtg gcgttcacgc cccggtcaca gtcgctgcga gacgccgcgc cagcgattcg
    13021 gccagcgcat cgtcgcccgc ctcgaccatc acgcgcagca ccggttccgt gcccgatgcg
    13081 cggatcagca cgcggccgga gccgtacagc tgcttctcga ccaactcgat ctcgcgcgcc
    13141 agcggcgcgt gctgcttcca gtcgaattgg tttgcgatgc gcacgttgac cagcgcttgc
    13201 ggcagcagcg gcagatcggc ggtcagctgc gcgagcgtct gccgcgcgcg ccgcatcgcc
    13261 gccagtacct gcaggctgct gatgatgccg tcaccggtcg tgtgcttgtc caggcagagg
    13321 atgtgtcccg agccttcgcc gccgagcagc cagccgcgct gctcgagcag ctccagtacg
    13381 tagcggtcgc cgaccttcgc gcgctcgaac ggcacgcgca gctcgcccag ccggcgctcg
    13441 agcgcgaagt tggtcatcag ggtgccgacg gcgccgcgca cgccattgat cgccaggcga
    13501 tcgcgcacga tcacatacag cagttcgtcg ccgttgtaga cgcggccgct gccgtcggca
    13561 atcaccacgc ggtcggcgtc gccgtcgagc gcaatgccga agtccgcgcc gtgcgcacgc
    13621 acgacttcgg acagatgtgt cgcgtgcacc gcgccgacac cgtcgttgat gttgaagccg
    13681 ttgggctcgg cgccgaccgc gaccacctct gcaccgagct catggaacac gtccggcgcg
    13741 acgtgatagg cggcgccgtg cgcgcagtcg acgatgatct tcaggccctt caggtcgaat
    13801 tcgttgggaa acgtgctctt gcagaactcg acgtagcgcc ccgccgcgtc atcgagccgg
    13861 cgcgccttgc cgagcaggcg cgactcgacg cactcctgcg tgcggtccat ttcctcctcg
    13921 atcgcctgct ccgtgtcgtc cggcagcttc ttgccctgcg ccgagaagaa cttgatgccg
    13981 ttgtccggat acgggttgtg cgaagcactg atcaccacgc cggcggtcag ccgcagcgcg
    14041 cgcgtcaggt acgcgaccgc gggcgtcggc agcgggccgc acagcacgac gtcgacgccg
    14101 acgctggaga agcccgcttc gagcgccgcc tccagcatgt agccggagat gcgcgtgtcc
    14161 ttgccgatca gcacggttgg ccgctgaccc gcgatgcgcg cgagcacgtg cccggcggcc
    14221 tgtccgagtc gcatcacgaa acccggcgtg atgggcatct gtccgaccgt gccgcgcacg
    14281 ccgtcggtgc cgaagtactt tcgactcacg tagtcccctc ttcaaatgcc cgcagcgccg
    14341 cccagacgcg caatgcgtcg cgcgtggcgg caacgtcgtg cacgcgcacg atgttcgcgc
    14401 cgcgctggac cgccagcaac gcagcagcga ccgacgcgtg caggcgctgg tcgaccggcc
    14461 gccccgtcag ctgcccgagc atcgatttgc gcgacaaccc cgccagcacc ggctgcccga
    14521 gccgcgccag cgcgtcgagc cgcgccagca aggcgagatt atgcgcgacc gtcttgccga
    14581 atccgaatcc cggatcgagc gcgatgcgcc cggcgcccac tccggcttgc acgagcgcat
    14641 cgcggcgcgc acgcaggaac tcgacgacct cgcgcaccac gtcctcgtag tgcggtgccg
    14701 cctgcatcgt gcgcggcgaa ccctgcatgt gcatcagcac cataccgcag tcggtgcgcg
    14761 ccgcgactcc gaccgcgccg ggcgcctgca gcgcgcgcac gtcgttgatg atcgaggcgc
    14821 cctcggcgag caccgcgtgc atcacctcgg gcttcgaggt gtcgaccgag atcggcacgc
    14881 cggcttcgcg cagcgcccgc accagcggca gcacacgctg cagttcctcc tccacgccaa
    14941 ccgcggccgc gccaggccgc gtcgactcgc cgccgatgtc gaggaggtcg gcgccctcct
    15001 cgatcagccg ctgcgcggta tcgcgcgcgg cgactggatc gaggttgcgg ccaccgtcgg
    15061 agaacgagtc gggcgtgacg ttgatcacgc ccatgatgag aggccgcgtc agcggcaact
    15121 ggaaccgacc gcagtgccac gagggcgtcg tcataaacaa caagggccgg ttgcccggcc
    15181 ctgcggattg tgtcgattga cgatcacgcc ggctgcgccg cgccctcggc gccaccggac
    15241 tcggacttgc cgcccgtcgt cgaggtcgac gacatcggct tcggcggccg cggcggcttg
    15301 cccgccatga tgtcgtcgat ctggtcggca tcgatcgtct ccagttcgag caacgccttg
    15361 gccatcgcct cgaccttgtc gcggttgtcc tcgatcagct tgcgcgccag cgcgtactgc
    15421 tcgtcgatga tgcggcggat ctccgcgtcg accttgcgca tcgtctcttc cgacacgtgc
    15481 gtggtcttgg tgatcgagcg accaaggaag acctcgccct cattctccgc atacaccatc
    15541 gggccgagca cgtcgctcat gccgtagcgc gtgaccatgt cgcgcgccat ctgcgtggcg
    15601 cgctcgaagt cattgccggc gccagtcgtc atctggctca tgaacacctc ttcggcgatg
    15661 cggccgccga agagcaccgc gatgcgcgac agcaggtaga cgcggtcgta cgcgtagcgg
    15721 tcctgctcgg gaagctgcat ggtgacgccg agcgcgcggc cgcgcggaat gatcgtcacc
    15781 ttgtgcaccg ggtcggactt cggcacgagc ttcgcgacca ctgcgtgccc ggattcgtgg
    15841 tacgcggtgt tccggcgctc ttcctcggac atgaccatcg acttgcgctc ggcgcccatc
    15901 atgatcttgt ccttggcgcg ctcgaagtcc tgcatctcca cgacgcgggc gctgcggcgc
    15961 gcggcgaaca gcgccgcctc gttgacgagg ttcgcgaggt ccgcgccgga gaatccgggc
    16021 gtgccacgcg cgatcacgtc ggccttcacg tccgcgccga tcgggacctt gcgcatgtgc
    16081 acgttcagga tctgctcgcg tccgcgaatg tcgggcagcg gcacgacgac ctggcggtcg
    16141 aagcggcccg gacgcagcag cgccgggtcg agcacgtcgg gccggttggt cgcggcgatc
    16201 acgatgatgc cctggcccgt ctcgaagccg tccatttcca cgagcagctg gttcagtgtc
    16261 tgctcgcgct cgtcattgcc gccgcccagc cccgcgccgc gctggcggcc gaccgcgtcg
    16321 atctcgtcga tgaagatgat gcacggcgca tgcttcttgg cgttttcgaa catatcgcgc
    16381 acgcgcgccg cgccgacgcc gacgaacatc tcgacgaagt cggaaccgga gatcgagaag
    16441 aacggcacct tcgcctcgcc cgcaatcgcc ttggcgagca gcgtcttgcc ggtgccgggc
    16501 gagccgacca tcagcacgcc gcgcggaatg cggccgccca gcttctggaa cttggtcgga
    16561 tcgcgcagga agtcgaccag ctcctgcacc tcttccttcg cctcgtcgca gccggcgacg
    16621 tcgacgaacg tgatggtgtt gttgctctcg tcgagcatgc gggcgcgcga cttgccgaag
    16681 ctgaacgcgc cgccgcggcc gccaccctgc atctggcgca tgaagaagat ccagacgccg
    16741 atcaggagca gcatcgggaa ccacgacacg aagatcgagg tgagcagcga ctgctcctcg
    16801 cgcggtttca catcgacttt gaccccggcg ttgatcaggt cgcccatcag gccgcggtcg
    16861 agcacggtgg ccacggtacg gatgcggcgg tcgtcattgg tcaccgcgac cacctcgggc
    16921 gtgacgcccg actcctggat cgtcaccgac ttgacgcggc ccgacttcac ctcggccagg
    16981 aaatccgaat acggaagata cgattcaccg ggccgctgcc ggccctcgaa ctgcttgaat
    17041 acagtgaaca gaacgagcgc gatcaccatc cagatggcgg ctttcgcgaa catgttgttg
    17101 ttcaagatga actcctgata acgggcgctg cgcccgaggc ggctcgatcc tgcgcacccg
    17161 agccctgatg cacggccatt gtaggcgcaa tctatccgcc tccatcccac tggcgcgact
    17221 ggaatgtggg ggcgatcggc gggctatttc aacccttcag ccgtttgttc cgtctccggg
    17281 ttgaccctca ccttcaatcc gcgcgccagc aggtacgtct ccgccgattc ggtccgcgat
    17341 gcgggcggtt ttcttgccgc gaccgaaacg aaactacgct tcaactgctc gacgaactgg
    17401 ctgtaaccgc tgccctggaa ggctttgacc agcaagacac cggccggttt caggtgcgcc
    17461 tgcgcgaact cgagcgcaag ttccgccaga tgcaggctgc gcgcggcatc ggcagcggcg
    17521 atcccggaca gattgggcgc caggtccgac agcacgacgt cggccttctg tcccgccaat
    17581 gcggcggcaa gctcggcagc gaccggctcc tcgcgaaaat cgccctggat ggcctgcacg
    17641 tcgggtagcg gatccatcgg caggatgtcg agcgcgacga tgcggccgcg cacgccatac
    17701 tttgtcatca gccgctcgcg cacgacctgc gcccagctgc cgggcgcggc tccgaggtcg
    17761 atcacagtca tccccggccg cagcagcttg tccttggcgt cgatctcgat cagcttgtag
    17821 gcggcgcgcg agcggtagcc gtcgcgggtc gccgccttca cataaggatc ggtcacatgg
    17881 cgacgaatcc acccgggatt ggagcgatgc ttctttttcg ctacgggcat gccggtagca
    17941 tacgcagatg gccgaactga atctgacgcg ggacgaccgc ctggcgctga aggcgcgcgc
    18001 gcaccacctg aatccggtcg tgctgctcgg cgcttctgga ttgtcggacg ccgtggtcag
    18061 ggaaatcgac cgcgcactca atgcgcacga gctgatcaag attcgcgtgc ccggcgaaga
    18121 ccgcgacgag cgcgagcggc tgttcgccga tctggccgag cggctcgggg ccgcccgggt
    18181 gcaagcgatc gggaagctgc tcgtgcttta tcggccgaaa ccagcggagg ttgcacccgc
    18241 gaaaccggtg ccgcccaggc gcaccgaacg cgcatcggcg gcacgcgcca agcctcgacg
    18301 cgatgaaatc cgccgcacgc aacgccgcgt ggcagcgccc cggggtcgac gtcaggcgta
    18361 gctgaccatc aggatctcgt actcacgccg cccgccggga gcggccacct cgatcacgtc
    18421 gccctcgacc ttgccgatca gcgcgcgtgc gatcgggctg gagatcgaga tcagcccggc
    18481 cttgatgtcg gcctcgtcgt cgccgacgat ctggtacttg accgattcgc ctgcggtcag
    18541 gtcctccagc tccacggtcg cgccgaacac gacacgacca cccgcatcga cggtggccgg
    18601 atcgatgatc tgcaggctgc cgaccttgcc ttcgagttcg ttgatgcgcc cctcgatgaa
    18661 gccctggcgt tcgcgcgcgg cttcgtactc ggcgttctcc gagaggtcgc ccttttcgcg
    18721 cgcctccttg atcgcctgga tgatcgcggg ccgatcgacc gccttcaatc gttgcagctc
    18781 ttccttcagt cgttcggcgc cacgcaccgt gattggaatc ggcatttcgc aaactcgcag
    18841 aaaacacaaa gccccggcgc tgccggggcc gacgagcttg gactttatcg accccgctcg
    18901 ggcggcgcaa gcggcagggc gcactgccgc tgtcgcgcga tgttcctcca tgggctcaat
    18961 gtcccgcccg aggacctttt gccgatgcac gaactcgtcc gcatcaggtg gtggatgaag
    19021 tcctcgacca gcatgcgaag attccgcaac gatcgatgat tccgttctcc attctcgacc
    19081 tcgcgccgat agtcgtcggc ggcgacgccg cccaggcgct cgccaacacg ctcgacctcg
    19141 cgcgccacgc cgagcgctgg ggctatcgcc gctactggct ggccgagcat cacaacatgg
    19201 acggcgtggc gagctcggcc acggcggtgc tcgtcgggca ggtggcaaca gtcacgcagt
    19261 cgatccgcgt cggctccggc ggcgtgatgc tgcctaacca ttctccgctg gtgattgcgg
    19321 agcagttcgg cacgctcgct acgctctttc ccgaccgcat cgacctcggg atcggccgcg
    19381 cgccaggcac ggacccgctg acgatgcgtg cgttgcgccg gcacctggcg caggaggacg
    19441 acgacttcat cgcagacgtg cgcgaactgc agtcgtatct cggaccgccg gttcctgccg
    19501 cgcgcgtgcg cgcgattcct ggcgtcggca cgaatgttcc gatctggctg ctggggtcga
    19561 gcctgtacgg cgcgcaggct gccgccctgc tcggcctgcc gttcgcattt gcatcgcact
    19621 tcgcaccggc gctgctgcca caggcgctgg cgatctaccg cgagcgcttc cgaccttcgg
    19681 ccacgctgga acggccgtac gcgatggtcg gcgtcaacgt gatcgcggcc gacaccgaca
    19741 cctcggcggc gcgtctgttc acctcggcgc agcagcgctt tctcggcatg acgcgcggcc
    19801 tgcgcgggcc gctgccgcga ccggtgccga ccatggatgg actgtggaac gaggacgaac
    19861 gcgcgcaggt cgagcaaatg ctcgcggtgt ccgcagtcgg gtcgcccgca acgatcgagc
    19921 accggctcgc cgagctggtc gaagcgaccg gcgccgatga gctgatcgtc gccgcagcga
    19981 ttcacgatca tgcggcgcga ctgcggtcgt acgaactgct ggcgcagatc gggtgcgcgc
    20041 tacgccagtg aacggtgcag cgcctgcagg tcgtagacct cgagccggtc gaggtacttc
    20101 atgccctcga ccgctgcgcg accgccggcg atcgtcgtgt agtagttcac gcgctgtgcc
    20161 agcgcggtcg tacggatcga acgcgagtcg gcaatctgcg cgcgattctc tgacaccgtg
    20221 gtgatcacga gatcgatctc gccgttcttc agcagatcga ccacgtgcgg gcggccgtcc
    20281 ttgaccttgt tgaccggctt gaccggtatg cccgctgcct cgatcgcggc cgccgtgccg
    20341 cgtgtcgcaa ccagctggta gcccatctcg tgcaacatgc gcgcgacctc gacggtgcgc
    20401 ggcttgtcga cgtccttcac gctcaggaac acggtgccgg tggccggcag ccgcgccgag
    20461 gcgccgagct gtgatttgga cagcgcctcg ccgaagttgc gtcccacgcc catcacctcg
    20521 ccggtcgagc gcatctcggg cccgagcagc ggatcgacgc cggggaactt cgcgaacggg
    20581 aacacggcct ccttgaccga gaagtacggc ggcacgacct cgacccgtgc gccctgcgcg
    20641 gcgatcgatc gtccgaccat gcagcgcgcg gcgatcttcg cgagcggttc gccggtcgcc
    20701 ttcgagacga acggcaccgt gcgtgatgcg cgcggattga cctccagcac gtagacggtg
    20761 ccgtcctgga tcgcgaactg cacgttcatc aggccgacga cgttgagccg ctcggccatg
    20821 agcgtggtct ggcgcttcag ttcctcgatc atcgcggcgc tcaacgaata cggcggcagc
    20881 gaacaggcgg agtcgcccga atgcactccc gcctgctcga tgtgctccat cacgccgccg
    20941 atgcggacct cacggccgtc ggccacgcag tcgacgtcga cctcgatcgc atcgtcgagg
    21001 aaacggtcga gcagcaccgg tgaatcgttg ctgaccttca ccgcttcgcg catgtagcgt
    21061 tcgagatcct tgtcgtcctg cacgatctcc atcgcgcggc cgccgagcac gtagctcggc
    21121 cgcaccacca gcggatagcc gatctcctgc gcgagcttca gcgcctcgtc ctcggtgcgc
    21181 gcggtgcggt tcggcggctg cttgagctcg agcttgtgca gaagctgctg gaagcgctcg
    21241 cgatcctcgg cgatgtcgat cgaatccggc gtagtgccga tgatcggcac gccgttggct
    21301 tccagatcgc gcgcgagctt cagcggcgtc tgcccgccgt actgcacgat cacgccgaac
    21361 ggtttctcct tgtcgacgat ctcgagcacg tcctccagtg tcagcggctc gaagtagaga
    21421 cgatcgctgg tgtcgtagtc ggtcgatacc gtctccggat tgcagttgac catgatggtc
    21481 tcgaacccgt cctcgcgcag cgccagcgcc gcgggcacgc agcagtagtc gaactcaatg
    21541 ccctgcccga tccggttcgg gccgccgccg agcacgatca ccttcttctt cgacgtgggg
    21601 ttcgcctcgc attcgtcctc gtaggtcgag tacatgtacg cagtcgtggt cgcgaactcc
    21661 gccgcgcagg tgtcgacgcg cttgtagacc ggccgcacac cgagcacgcg ccgcgccccg
    21721 cgcacgtcgt cggccttcgt acccagcagc cttgccagcc tgcgatcgga gaaacccttg
    21781 cgcttcagga agcgcatttc ctcggcggtc agcgagccga gcgagcgacc cttcaggcgc
    21841 gcctcgatcg cgaccagttc ctcgatctgc gccaggaacc acggatcgat cgccgtttcc
    21901 tcgcgaacct cggcgaggct catgccgatg cggaacgcgt cggcgacgaa ccagatccgc
    21961 tccggcccgg gatcgccgat ctcctcgacg atctcgtcgc ggttcgtcga atgctcgtcc
    22021 agcccgtcgg caccgacctc cagcccgcgc agcgccttct ggaacgattc ctggaacgtg
    22081 cggccgatcg ccatgacctc gccgaccgat ttcatctggg tcgtcaggtg cggatcggca
    22141 gcgcggaact tctcgaacgc gaaacgcggc accttggtga ccacgtagtc gatcgtcggc
    22201 tcgaacgacg ccggcgtggc accgccagtg atctcgttgc gtagttcgtc gagcgtatag
    22261 ccgaccgcga gcttggccgc gaccttggcg atcgggaatc cggtcgcttt cgacgccagc
    22321 gccgatgaac ggctgacgcg cgggttcatc tcgatcacga tcatgcgccc ggtcccggga
    22381 tcgatcgcga actgcacgtt ggagccgccg gtatcgacgc cgatctcgcg caggatcgcg
    22441 atcgacgcgt tgcgcatcag ctggtattcc ttatcggtca gcgtctgcgc cggcgccacc
    22501 gtgatcgagt cgccggtgtg gatccccatc gggtcgaggt tctcgatcga gcagatgatg
    22561 atgcagttgt ccgccttgtc gcggaccact tccatctcga actctttcca gccgagcagc
    22621 gattcctcga tcagcagttc cctggtcggc gacaggtcga gcccgcgcgt gcagatcgcc
    22681 gtgaactcct ccatgttcca agccacgccg ccgccgctgc cgccgagcgt gaagctcggt
    22741 cgaatcacga ccggaagacc catcgccttc tgcaccgcga gtgcctcgtc catcgagtgc
    22801 gcgatgcccg agcgcgcgga ggcgagcccg atgcgggtca ttgcctcctt gaacttctgc
    22861 cggtcctcgg ccttctcgat cgcgtcgggc gaggcgccga tcaactcgac gtggtgtttg
    22921 tgcagcacgc cgtgcttgta caaatccatc gcgcaattga gcgccgtctg cccgcccatc
    22981 gtcggcagca ccgcgtcggg cttctccttc gcgatgatcc gttcgaccac ctcccacgtg
    23041 atcggctcga tgtaggtgac gtccgccgtc tccgggtccg tcatgatcgt cgccgggttg
    23101 ctgttgacga ggatgacctt gtagccctcc tgcttcagcg ccttgcaggc ctgcgcgccc
    23161 gagtagtcga actcgcacgc ctgcccgatc acgatcggcc cggcgccgat gatcaggatc
    23221 gattggatat cggtgcgctt aggcacgggc ctcccgccgg gccgccccaa gggaggcccg
    23281 cgccccctcg gggggcagcg aatgaattga gcgtgggggc atctaccgct cttccatcag
    23341 cttcacgaag cgatcgaaca agtaaacgat gtcgtgcggc cccgggctcg cttccgggtg
    23401 gccctggaag cagaacgccg gcttgtcggt gcggcgaaag ccctgcagcg tgccgtcgaa
    23461 aagcgacaca tgcgtgacgc ggcacgtgtc gggcagcgtc ttcggatcga ccgcgaagcc
    23521 gtggttctgc gacgtgatca gcacgcgccg atcgtcgagg tcctgcaccg gatgattggc
    23581 gccgtgatgg ccgaatttca tcttcagcgt cctgccgccg gaggcgagcc ccatgatctg
    23641 gtggccgagg cagatgccga aggtcgggta acccttcgcg atcagctcgc gcgccgcctc
    23701 gatcgcgtaa tcacacggct cggggtcgcc cggtccgttg gacaggaaca cgccgtcggg
    23761 ctcgagccgg atcacttctg cggccggcgt ctgcgccggc accaccgtca cgcggcagcc
    23821 gcgctgggcg agcagacgca ggatgttgcg cttcacgccg tagtcgtagg cgaccacgtg
    23881 gcgcttcggc tcggtgagct tgccgtaacc cgcgtcgaga gaccacgcgg cctcgcgcca
    23941 ctcgtacggc gcatcggccg acaccacctt ggccagatcg agccccgcca tgccgggaaa
    24001 cgaccgcgcc agcgcgaccg cgcgcgtctc atcggtgccg accaggatcg cgcccggctg
    24061 cgcgcccttg tcgcgcagcg cgcgcgtgag cttgcgcgta tcgatgtcgg cgatcgcgac
    24121 caagttctcg cgcaccagat actcggtcag cgacgcctgg gcgcggaaac tcgaatgcag
    24181 gggcggcaga tcgcggatca cgagtcccgc cgcgaacacc ttgaccgact cgacgtcctc
    24241 ggcgttggta ccggtattgc cgatgtgcgg ataggtcagc gtgacgatct gccggcagta
    24301 gctcgggtcg gtcaggattt cctggtagcc ggtgatcgcg gtgttgaaga cgacttcccc
    24361 gacacaatgg ccggcggcgc cgatcgagcg gccgcgaaag atcgtcccgt cggccagcgc
    24421 cagcagcgcg gcgggacggg agtcggagat cagggaagac aaacgggctc ctctgctgcg
    24481 acggcggcgc tcagcgatgg ccggtccgct gacggcgacg cgtgacgcgc actccgtccg
    24541 ggccgcgccg cgcgatcgct aaactgcaga agtatatcgc ggcgcccgat gctgatcctt
    24601 ccctaccaga cccgattcgc gctgcgctcg acgccggtcg tcacgctggc gctgatcctg
    24661 atcaacgcgc ttgtcttctt cgttgcgcag gcgcgcgacg accgcgttta tgaagaggcc
    24721 taccgcttct atgaccggtc ggctctgccg cgcatcgagc tttcacacta tcgcgactgg
    24781 ctggcgcgcc aggcgaagga tcccgacagg cgcgatcagc tcgccgacct gcaggaatct
    24841 ctgcgcgagc aggacttcga gggcgcgctg gcgctaatgc agagcgacga cgacttcatg
    24901 cgttcgctgc ggcagctgaa cgttgtcacg cccggcgatc ccgagttcct cacctggcgc
    24961 gaccagcgca atcaatatga ggcgctgctg catagttcgg cgaccgagcg cttcctgctc
    25021 aagcgcgagc agcttcagcc atggcgcttc gtcacctacc agttcctgca cggcgatctc
    25081 ggtcactggc tcgggaacat gatcgtcctg ctgctggcgg gttcgttcgc agaggccgtg
    25141 ctcggacgct ggcgctttct cttcgcgtat ctgctctccg gcgtgggcgc cggcgcgctg
    25201 caggtcgcgc tgtccgacct gccggtgatc ggcgcgtcgg gcgcgatcgc gggcgcgatg
    25261 gcgatggtcg cggtgctcta tgggctgcgc cgtgtaccgg tgttcgtttc ggtgctgttc
    25321 tacttcgaca cgttcaggat gccggcgttg tggctgctgc cgatctggat cgcaaacgaa
    25381 ctctggcaat gggcgaatgc ggccgaggaa tcggttgcct acgcagcaca cctcggcggc
    25441 ttcgcgatcg gcgcgctgct tgcctggatg ctgcggccgg cgcgcagcct cgaaccggcg
    25501 tccgagcccg aggccgacag cggcggtgac gacgcccgcc tgcggctgca gcagcgtgcc
    25561 gaacaggccg ccgccgcggt cgacattccg cgcgccacgc atctgtatgg cgagctcgcc
    25621 gcccagtacc cgatgcgttc ggactacgcc gtggcgagct acaacatcgc actgctcgga
    25681 gccgatcgca acgcgctgcg caacgcggcg gagcacgtac tggtcaatcg tcccggtcgc
    25741 gcgaatgccg agttgcgtcg tgcttacctg cacatggcgc agtcgaagtc gcgcgacctg
    25801 ctgcccgtcg aggcgcagct gcggctcgcg cgccggctgg tgcgcgcgcg cgaggacggc
    25861 gccgcgctgc aattgatcga tgcgctgctt gccgacgaca agcggcgcac cgagcatgcg
    25921 cgcgcgctgt ccgattgcct gctgggcctg cacacgacct acctgcgtta tgggctgacg
    25981 ccgcaggccg acgacgtgcg ccgccggctc gcgcaatact tcacgccgcc cggcgcgggc
    26041 gcggcgccga tgcccgcacc gagcggccac actaccctac aggggccgtc gacgctgaac
    26101 ttcgatcttc cctgagcgag ccgctcagtc gcggcgtgcc gccgcagtcg cggcgagatc
    26161 gcgcatctcg cgctccgccg cttccgcctg cccgctttcc gcgagtgcct gcgcgcgcat
    26221 cagcacgacg gtcggcgccg agtcgtcgcc gccgtggcgc gattcgaact cgcgcgtcag
    26281 cgctagcacc aggtcgaact gcctcgcatc gagcgcggcg cgtgccaggc gcaccgccag
    26341 cgcacccgac gcaggcgtcc agtccgggtc gcctgccagc acgtcgcggg ccaatgcgag
    26401 cgcctgcccg ggctgccgct gcagcagcac ttcgaggtag cgctccagat gcgtgtcgcg
    26461 gcgtgcgcga tcaccggcaa gcgccagcag tctgcgcagg cgctcgtgca cggcggtgtc
    26521 gtcgggccgc gcaatcgatt cgcgctcgag aagttcgatc gcgctgccaa tgtcccccgc
    26581 agccatcagc cgcgccagcg ccgtatcgcg ccatccggcc gttgctgcct cggccgccgc
    26641 ctcttccccg gggccggtga cctgcaggcc gagcacgtcg ctgtactggt acagcacata
    26701 acccatcagc gcgcacatga tgaagaagaa gtaattgccg acaaaggagc ttgccagcgc
    26761 gagcagccat gccgattcgc cgagcgccgg cgccacgagc tcgaacgcct gctgcgaccc
    26821 gcccatcagc agcagcagaa acaggaagag cacgaggtac ggcgcgccga tgcggcgcgc
    26881 cagatcgaaa acgcggcgcg gattgattgc ctgccgcagg ctgtcggtca ccgccagcgt
    26941 catgatgcaa gcgggcagca gcagcgcgac cgcgatgtca gcgacaagcg tcaggacgcc
    27001 gtcgccggtg acgctcgtca cgagcgcgat gccgatgaag gccgtcagca tgatcgcggc
    27061 gagcttcagc ggccgccacg gatggatcgg ccgcgcggcg ggaaaccggt ccggatgcag
    27121 ataaccctgc gcgacgcgtt cgatcaccag gaagccgtag tacgcgagat agatcgaggc
    27181 gccactcgcg accagcgagg cgagccagcg cgactgcagc gcgagcgcgc ccgcgagcag
    27241 cgccgacaat gccaggttgc gcagcagcgg cgcgcgatag gcggggaagc ggaagaactg
    27301 cggcatgcgc cgccagaacg gtgtggccgg cacgcgcggc accggcgatg gcgcgtcggg
    27361 gctgaaggac gtggacacgg tgtgcggcgg cgtgcagccg cgcttcagag tttttccgtc
    27421 tcgcccgagc gcggctgcca ccgcatcagg cgcttctcgc cgacgccgac cacccaatct
    27481 agtaccagcg caaagcccgt cagcacgaga atacccgcca tcaccgtgtt gatgtcgaac
    27541 gtgccctcgg cctgcaggat caggtagccg acgccgcgcg ccgagccgag gtattcgccg
    27601 accaccgcgc cgacgaacgc cagcccgacc gacgtgtgca gcgacgaaaa cacccagctc
    27661 atcgcactcg gcagatacac gtgacgcagc agctgccgcc ggttcgcgcc cagcatgcgc
    27721 gcgttggcga gcaccgtggg gctgacctcg cgcacgccct ggtagacgtt gaagaacacg
    27781 atgaagaaca ccagcgtgaa gccgagcgcg accttgctcg ccacccccag cccgaaccac
    27841 accgcgaaga tcggcgccag aatcacgcgc ggcatcgaat tggccgcctt gatgtacggg
    27901 tcgaggattc ccgccgccat cggcgacagc gcgagccaca tgcccatcgc cagtcccgcg
    27961 ctggtgccga tcgcgaacgc gagcaccgtt tccagcagcg tgacccacag gtgctcgtag
    28021 atgtcgcgct gggcgaagaa ccagttccag atgcggccga agatcgccag cggttcgccg
    28081 aagaagaagg ccgcctgccg gtcgttttcg aacatgaacg gcgggatgag tcccggcctc
    28141 gtcatcatgt gccagaacac gaacagcagc acggccacgc cgacctgcca gacgcggagg
    28201 ttgcgcggat tgggcttgat gatcgaccac atcgcggtcg acggatcagg caagcagttc
    28261 ttcgacggtg gcgacgcgcg cgaacgcgcc gttcagcgcg gcgaggaacg ccgcgtgcac
    28321 ctgcggcgcc ggcaccgtct gcccgcccca ttgcagatca cgcgtcgcgc aggcgtccgc
    28381 cgcgacgctg cagcgaaaac cgagatcggc cgcggcgcgc accgaggtgt cgatgcacat
    28441 gtgcgtcatc atgccgacga acagcagttc gtcgacgcct gctgcgcgca attgcccggc
    28501 gagcgaggtc tcgcgaaagg cgttggggaa gtgcttgacg atcagcgact cgcccggatg
    28561 cggggcaacg gccgtgtgga tctgcacgcc ttcggtgtcc ggcagaaaga acgtcgcgcc
    28621 ggcacgcgtc gacacgtgct ggacatggaa cacgggcagg ccgcgctcgc gctgccattg
    28681 cagcacgtgt gccgcgcgcc cggccgcagc cgctggcccg tccagcggca tgcggccgcc
    28741 gggaaagtag tcgttctgga tgtcgatgat gaccagcgcc tgtttcatca tgcggccctc
    28801 ttctgctgtt cgtaaccctt caggacttcc tcgcgcagca caccccagat cgccttgtgc
    28861 agctcgatga accgcggcgt cacgcgcacc tcggccacgt cgcgcggacg cggcaggtcg
    28921 atcgcgaatt cgccgatcgg atgcgacgcc ggtccggccg acagcacgat cacgcgatcg
    28981 ctcatcgcga tcgcctcgtc caggtcgtgc gtgatgaaga gcaccgcctt cttcttcgcc
    29041 gcccacaggt cgagcacctc gttctccatc agctggcgcg tctggatgtc cagcgcggag
    29101 aacggctcgt ccatcaggat tatgtccgga tcgagcacca gcgtctgcgc cagcgacgtg
    29161 cgcttacgca tgccgccgga caactgatgc ggatagcggt cgccgaagcc gccgagcccg
    29221 acgcgcttca gccacgcgtc cgcctgcgcg cgcgcctcgg ccgacggcac accacggaac
    29281 tccagcccgg cgctgacgtt cgcaagcgtc gtgcgccacg gcatcagcgc ttcggactgg
    29341 aacatgtagc cggcacgcgc attgatgccc gcgagcggcg ccccgaacag ctcgaccgcg
    29401 ccgctgctcg gctcgagcag tccggcggcg acgttcagca gcgtgctctt gccgcatccg
    29461 gttggcccga ccacgctgac gaactcgccc gccccgaccg acaacgtgac ctcacgtacc
    29521 gccgtgtagc gctgccccgg cgcgtcgcgc gagatgaagg tgcaagtgat gcggtcgagg
    29581 gaaagagcgt tcatcctgac ttgtgcccga ttgacccgca ggccccgccg cgcgaacgtg
    29641 cgcgcgtcca ggaaccatga gtcacgggga acgtgtcact tgtacttggc cagcgccttc
    29701 ttcacgaatt cgttggtgta gttggccttc aggtccagct tggcggtctt catgctttcg
    29761 tcgacgctgg ccagcgcccg cagggcggtg gcggagcccg cgtcggggat catgccatcg
    29821 ggcgacagcg cgcccttggc cttcaggaac gcgtcgatgt agaccgcgcg gtcgcccagc
    29881 aggtacgact ccgggacgac cttgatgatg tccgacggcc cggctttctg aatccactgg
    29941 tcggcgcgca cgatcgcatt gaccagcgcc tgcgcggtgt tcgggttctt ctccaggaat
    30001 gacaccggcg cgtacaggca cgcggccggc atcggcccgc cgaagacctt gtcggcctcg
    30061 gccacgtcgc gcgtgtcgga gacgatcttg atgtcgccgc tgcgcgcgag gatcgtgatc
    30121 accggatcga ggttcgagat cgcgtcgatc tgcccggagc ggatcgccgc caccgcgccc
    30181 tgcgacgcgc cgacgccgat gatcgacacg tcggtcggct tcagcccggc cttggcgagc
    30241 acgaagttcg ccatcacgtt ggtcgacgaa cccggcgcgg tgacgccgat cttcttgccc
    30301 ttcagatccg caatcgactt gaagttcggc atcgtcttgg agttcacccc cagcacgatc
    30361 tgcggcgcgc gcccctgcag cacgaatgcg cgcatcggct gccccttggc ctgcatgttc
    30421 agcgtgtgct cgaaggcgcc ggacaccacg tcggcgctgc cgcccaccac cgcgcgcagc
    30481 gcctgcgcgc cgccggcgaa gtcgacgatg ctgatctcca gcccctcggc atcgaaatac
    30541 ttgagctgct cggcgaccgt cagcggcagg taatagagca ggttcttgcc gccgaccgcg
    30601 atcgtgacct tcttcttttc cagggcctgc gcacgcacga acgcaggcgc ggcgatcgcg
    30661 gccgcgcccg cgagaagaag attgcgtcga gttgtttgca tcaagatctc ctatttgtca
    30721 tatcacacca tcttttctga gtgcggcaag ctccgattca tcataaccaa gcgcggcgag
    30781 cacctgatcc gtgtgctcgc ccaggcgcgg gccgagccag cgcgtctcgc cgggcgtggc
    30841 cgaaagcttc ggcgtgaccg cgggcaccgc cagatcggtg ccgtcgggca gccgatggcg
    30901 ttcgatcatc gcgcgcgccg caaactgcgc gtcgcggaac atatcggcga cgctgtagat
    30961 cttcgacacc ggcacgtcgg ccgcggtcag cgccgccacg gctgcatcga tgttccggcc
    31021 cgagcaccat gcctggatcg cggcgtcgat ctcctgcgtg cgcggcacgc ggccatcgtt
    31081 gcgcgccaga gccgggtcat tggcaaggtc ggcacggccg atcgcggtca tcagccgctt
    31141 gaagatcgcg tcgccgttac cggcgatcac gatcgcggcg ccgtcggcgc aggtgtaggt
    31201 gttcgagggc acgatgcccg gcagcgcgcc cccggtccgc tggcggatca cgccggcgcg
    31261 gtcgtactcg ggaacggtgc tctccatcag gttgaacaca gcttcataga gggccacgtc
    31321 gaccaactgc ccttcaccct tgccacggcc gccggtcgcg tcgcggtgaa gaagcgccat
    31381 cagcgcgccg atcacgccgt gcagcgcggc gatcgaatca ccgatcgaga tgccgatccg
    31441 catcggcggc cgatcgggga agccggacac gtagcggatg ccacccatcg attcgccgat
    31501 cgcgccgaat ccgggtttgt cgcgcatcgg cccggtctgc ccgtagcccg acagccgcac
    31561 catgatcgtc gccggattca gagcccgcag gtgctcgtac ccgaggcccc acttttcgag
    31621 cacgccgggg cggtagttct cgatcacgac gtcggcgtcg agcgcgagct tgcgcgcgat
    31681 gtcctgtgcg cgcctgtcct tcaggttcag cgtgactgac ttcttgttgc gcgcctgcac
    31741 gtaccaccat aacgacgtgc cgccgtgcag gtggcgccag gtgcgtatcg gatcgccgcc
    31801 gtcggtggct tcgatcttga tcacctcggc gccgaactcg gcgagcatgc gcgcagcgaa
    31861 cggccccgcg atcagcgtgc caagctcgag aaccttcact cccctcaaag cgctcatgcg
    31921 gcaccgagtc cggaaacgaa gatggtgatc gttgccagcc agccgacgag ccacagcacg
    31981 gaccgcgccc agtgccagtc cgccaggtag gaggccagat agccgaggcg cacgacgatg
    32041 aagatgaccg ccagcgcgct gatgcgctcg gccgacgcct gcgtcagatg cgcgacgatc
    32101 accgccgcgg cgaagaatgg aaaggcctcg aagctgttca gttgcgccgc gtgagcgcgg
    32161 gcacggaaac cctgctgctt cgccagccac gcgcgcgggt cccgattgtt gaaacgctcg
    32221 ccgcccgcct tggcgatcgt ggtcgccaga tacggcagaa tgcccgcgat cagcacgcac
    32281 cacagcgcca cggtcatcga gggttcctca ggccgcgcgg cgatcgagca tcgcctgcgc
    32341 cagcgtgttg acgtcggtgt actccagctc ggcgccggcc ggcacgccgc gcgccagccg
    32401 cgtgaccttc agttgcggat cgcgcgcgcg cagcagttcg gcgatgtaat gcgcggtcgc
    32461 gtcgccctcc ggcgtgaacg aggtggcaac gatcacctcg cgcaccaacc catcacacgc
    32521 gcgcgccagc aaccgttcca tctggatctc gcgcggcccc acgccgtcga ggggcgacag
    32581 ccggcccagc aggacgaagt acaggcccgc gtacgacagc gactgctcga gcatcagctg
    32641 gtccgacggc gactcgacca cgcacagcag gccggcgtcg cgcttgggat tcgaacaggt
    32701 ctcgcagatt tcctgctcgg tcagcgtatt gcagcgcgcg caatggcgca cgttggcgtg
    32761 ggccgcaagc agcgcctcgc ccagtcgaac ggcggtgtcg cgatcgtgct gcagcagatg
    32821 aaacgcgatc cgctgcgccg accgcggtcc gatgccgggc agacgtttca gcgagtcgac
    32881 cagtttgtcg agggtgggcc cgagtttcac actcagaacg gcagcttgaa tcccggcggc
    32941 atcggcatgc cggcggtgat cgaagccatc ttctgctggg tcgtttcctc
//
LOCUS       DBNHBY010000758         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_803194, whole genome shotgun sequence.
ACCESSION   DBNHBY010000758 DBNHBY010000000
VERSION     DBNHBY010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_803194"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC16HB_17875"
     CDS             complement(<1..729)
                     /locus_tag="AC16HB_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5608713.1"
                     /translation="MGTSETPTIDLQAILRETQVQGVIDQLDRDLIGLAPVKRRIREI
                     AAFLVVSRARKTLGLDVATPSLHMSFTGNPGTGKTTVALRMAEILHRLGYVRKGHVIS
                     VTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRPENERDYGQEAIEILLQV
                     MENQRDDLVVILAGYKDRMDTFFRSNPGLSSRIAHHIDFPDYTPDELLAIAQKMVAQM
                     NYRFDEGAAAAMSEYIALRIKQPHF"
     gene            complement(740..1159)
                     /locus_tag="AC16HB_17880"
     CDS             complement(740..1159)
                     /locus_tag="AC16HB_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO small subunit-like domain-containing
                     protein"
                     /protein_id="HIR5608714.1"
                     /translation="MRITQGAFSFLPDLTDAEIRAQVDYGLSKGWAWSVEYTDEPHPR
                     NTYWEMWGPPMFDLKDGAGVVYEINQCRKQHGDKYIRAMAFDSTRGVESIQLAFIVHR
                     PKDEPGFRLVRQETQGRSVRYTTEPYAAARNPEGQRY"
     gene            complement(1174..>2168)
                     /locus_tag="AC16HB_17885"
     CDS             complement(1174..>2168)
                     /locus_tag="AC16HB_17885"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HIR5608715.1"
                     /translation="GIVVERERLNLYGRALLGATMKPKLGLSGRNYGRVVYEALKGGL
                     DFTKDDENINSQPFMHWRDRFLYCMEAVNKASAASGEVKGHYLNVTAATMEDMYERAE
                     FAKELGSSIVMIDLVIGWTAIQSMSNWCRRNDMLLHMHRAGHGTYTRQKNHGVSFRVI
                     AKWLRLAGVDHLHCGTAVGKLEGDPMTVQGYYNVCRESVTKQDLSRGLFFDQDWADLR
                     KVMPVASGGIHAGQMHQLLTLFGDDVVLQFGGGTIGHPAGIHAGATANRVALEVMTKA
                     RNEGRDIVNEGPDILAAAARWCTPLAQALDTWKDVSFNYTSTDTPDFAVTPSVS"
BASE COUNT          329 a          715 c          706 g          418 t
ORIGIN      
        1 gaagtgcggc tgcttgatcc gcagcgcgat gtattcgctc atcgcggcag ccgcaccttc
       61 gtcgaagcgg tagttcatct gcgcgaccat cttctgcgcg atcgccagca gttcatcggg
      121 cgtgtagtcg ggaaaatcga tgtggtgcgc gatgcgcgaa ctcaggccgg ggttggaacg
      181 gaaaaaggtg tccatccggt ccttgtagcc ggccaggatc accacgaggt cgtcgcgctg
      241 gttttccatc acttgcagca ggatctcgat cgcttcctgc ccgtagtcgc gctcgttctc
      301 cggccggtac aggtagtacg cctcgtcgat gaacagcacg ccgcccatcg cgcgcttgag
      361 cacttccttc gtcttcggcg ccgtatgccc aatgtactgg ccgacgaggt catcgcgcgt
      421 gacgctgatc acgtggcctt tgcgcacgta accgagccgg tgcaggatct ccgccatccg
      481 cagcgccacc gtggtcttgc cggtgccggg attgccggtg aagctcatgt gcagcgacgg
      541 cgtggcgaca tcgaggccga gcgtcttgcg cgcgcgcgag acgaccagga acgccgcgat
      601 ctcgcggatg cggcgcttca ccggcgccag gccgatcagg tcgcgatcga gctggtcgat
      661 caccccctgc acctgcgttt cgcgcaggat cgcctgcagg tcgatcgtgg gcgtctccga
      721 cgttcccatc gatgtcgccc tagtagcgct gcccttcggg attgcgcgcg gccgcgtacg
      781 gctcggtcgt gtagcggacc gagcggccct gcgtctcctg ccgcaccagg cggaaacccg
      841 gctcgtcctt gggccggtgg acgatgaacg cgagctggat cgactccacg ccgcgcgtcg
      901 aatcgaacgc catcgcgcgg atgtacttgt cgccgtgctg cttgcggcat tgattgatct
      961 cgtagacgac gccggcgccg tccttgaggt cgaacatcgg tgggccccac atctcccagt
     1021 aggtgttgcg cggatgcggc tcgtcggtgt actcgaccga ccacgcccag cctttcgaca
     1081 ggccgtagtc gacctgcgcg cggatttcgg cgtcggtgag gtcggggaga aacgagaatg
     1141 cgccttgagt gatcctcatg tcgtgctccg tgatcaggaa acggacggcg tgaccgcgaa
     1201 gtccggcgta tcggtcgacg tgtagttgaa gctcacgtcc ttccaggtgt cgagcgcctg
     1261 tgcgagcggc gtgcaccacc tggccgcggc ggcgaggatg tcgggccctt cattgacgat
     1321 gtcgcgccct tcgttgcgcg ccttggtcat cacctcgagc gcgacgcggt tcgccgtcgc
     1381 accggcgtgg atgccggcgg ggtgcccgat cgtgccgccg ccgaactgca gcaccacgtc
     1441 gtcgccgaag agcgtcagca actggtgcat ctgcccggcg tggatgccgc cgctcgccac
     1501 gggcatcacc ttgcgcagat ccgcccagtc ctgatcgaag aacaggccgc gcgacagatc
     1561 ctgcttcgtc acgctctcgc gacacacgtt gtaatagccc tgcaccgtca tcggatcgcc
     1621 ttcgagcttg ccgaccgcgg tgccgcagtg caggtgatcg acgccggcga gccgcagcca
     1681 cttggcgatc acgcggaagc tgacgccgtg gttcttctgc cgcgtgtagg tgccgtggcc
     1741 ggcgcggtgc atgtgcagca gcatgtcgtt cctgcggcac cagttggaca tgctctggat
     1801 cgcggtccag ccgatgacga ggtcgatcat cacgatgctg gagccgagtt ccttcgcgaa
     1861 ctcggcgcgc tcgtacatgt cctccatcgt cgcggcggtc acgttcaggt agtgaccctt
     1921 gacctcgccg ctcgccgcgg atgccttgtt gaccgcttcc atgcagtaca ggaaccggtc
     1981 gcgccagtgc atgaacggct gcgagttgat gttctcgtcg tccttggtga aatcgagacc
     2041 gcccttcagc gcctcgtaga cgacgcggcc gtagttgcgg cccgaaagcc cgagcttcgg
     2101 cttcatcgtc gcgccgagca gcgcgcggcc gtacaggttc agccgctcgc gctcgaccac
     2161 gatgccgg
//
LOCUS       DBNHBY010000759         2982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1072773, whole genome shotgun sequence.
ACCESSION   DBNHBY010000759 DBNHBY010000000
VERSION     DBNHBY010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1072773"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC16HB_17890"
     CDS             complement(<1..373)
                     /locus_tag="AC16HB_17890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5608716.1"
                     /translation="MREYKKILACVDLTEGSERIVERARDVGARYGAELTLLHIVEYV
                     PVEPMGEALLPAVQIEGELVERATRRIAELAQKLGVEKCDRLVHAGNIKSEVVRVAQE
                     RNIDLIVLGSRERHGLSIMFNQ"
     gene            complement(464..1282)
                     /gene="rsmA"
                     /locus_tag="AC16HB_17895"
     CDS             complement(464..1282)
                     /gene="rsmA"
                     /locus_tag="AC16HB_17895"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR5608717.1"
                     /translation="MSRRPPADRSRFAKKRYGQHFLHDPGTIERIARAIDPQPGQSLV
                     EVGPGRGAITGPLLQRAGVLDVIEIDRDVLPLLEQRCRGLGELRVHVADALEFDFREL
                     RGSGPPLRLVGNLPYNVSTPLLFHFITQLDAFADMHFMLQKEVVQRMAASPGSGDYGR
                     LTVMLAPHVAVEPLFDIGTGAFQPAPKVVSTFFRLRPHPQPPFDVGDVDAFARVVAAA
                     FSRRRKTLRNALEGVLDAEGIRAAQVDPSVRAETLSPAQFAALAAQVPAAAEPR"
     gene            complement(1279..2307)
                     /gene="pdxA"
                     /locus_tag="AC16HB_17900"
     CDS             complement(1279..2307)
                     /gene="pdxA"
                     /locus_tag="AC16HB_17900"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR5608718.1"
                     /translation="MKPRTGTRRAPALRTLALTTGEPAGVGPDLCSALLEDLPRGCHI
                     VLIGDGSLLARSRRGRARKAAALAPFDLHAPRRAGVSLLHVPLAAPCTPGQLDPANAR
                     YVLDTLDAAIGGCAAGAFDAIVTAPVQKSVINDAGVRFTGHTEYLAQQTGAPLPVMML
                     VGGGMRVALATTHLPLRKVSAALKPERLAAVLRIVDADLRALFGVRRPRIAVLGLNPH
                     AGEGGHLGREEIEVIEPVLRALRADGLDLRGPLPADTAFLPRKLDEYDALVAMFHDQG
                     LPVLKHASFGHAVNFTLGLPIVRTSVDHGTALDLAGTRKADPGSLHAALALALDLARR
                     RQRGASHW"
     gene            complement(2304..>2982)
                     /locus_tag="AC16HB_17905"
     CDS             complement(2304..>2982)
                     /locus_tag="AC16HB_17905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5608719.1"
                     /translation="VYERARKNEDFGQLAVTYSDAGTALEGGALGWRKAGQLPSFVAD
                     LVPNMKAGDVTEPIRTPSGLHIFKVLEVRGGGGPAMVQQVNARHILLKPTEIADDQTT
                     KQKLEQIRQRILKGESFEAIASVTSEDPGSAANGGDLGWTGPGTFVPEFQKQIDALQI
                     NEISEPFRTQYGWHIVQVLGRRTYDASEDMLRNRCVSQLREARAEEETEMWLRRLRDE
                     AYVEYRM"
BASE COUNT          404 a         1005 c         1121 g          452 t
ORIGIN      
        1 tctggttgaa catgatcgac aggccgtggc gttcgcggct gccgagcacg atcaggtcga
       61 tgttgcgttc ctgcgcgaca cgcacgactt cggacttgat gttgccggcg tgcaccaggc
      121 ggtcgcactt ttccacgccg agcttctgcg cgagttcggc gatgcggcgc gtggcgcgct
      181 ccaccagctc gccttcgatc tgcacggcgg gcagcagggc ctcgcccatc ggctcgaccg
      241 gcacgtactc gacgatgtgc agcagcgtga gctcggcgcc gtagcgcgcg ccgacgtcgc
      301 gggcgcgctc gacgatgcgc tcgctgccct cggtcaggtc gacgcaggcc agtatctttt
      361 tgtattcgcg catgttttcg ctccgtgaaa cccggggctc cgccggcggt tgcgggtcga
      421 ttcgattata ggcccggggc cggatgcgcc ccaaatcgcc tggtcagcgg ggttccgccg
      481 ccgccgggac ctgggctgcc agggcggcaa actgcgccgg gctcagcgtc tcggcgcgca
      541 cggaggggtc gacctgcgcg gcgcggatgc cttcggcgtc cagcacgccc tccagcgcgt
      601 tgcgcagcgt cttgcgccgg cgcgaaaacg ccgccgcgac gacgcgcgca aaggcgtcga
      661 cgtcgccgac gtcgaacggg ggctgcgggt gcgggcgcag ccggaagaac gtggagacga
      721 ccttcggcgc gggctggaat gcgccggtgc cgatgtcgaa cagcggctcg acggcgacgt
      781 gcggcgccag catcaccgtg aggcgaccgt aatcgccgct gcccggcgag gcggccatgc
      841 gctgcacgac ctccttctgc agcatgaagt gcatgtcggc gaatgcgtcg agctgcgtaa
      901 tgaagtggaa cagcagcggc gtcgagacgt tgtagggcag gttgccgacc aggcgcagcg
      961 gcgggccgct gccgcgcagc tcgcgaaagt cgaactcgag tgcgtcggcg acgtgcacgc
     1021 gcagctcgcc caggccacga cagcgctgct cgagcagcgg cagcacgtcg cggtcgatct
     1081 cgatgacgtc gagcacgccg gcgcgctgca gcagcggtcc ggtgatggcg ccgcggccgg
     1141 ggccgacctc gaccagggac tggccgggct gcggatcgat ggcgcgggcg atgcgctcga
     1201 tggtgcctgg gtcgtggagg aaatgctggc cgtagcgctt cttcgcgaaa cgcgaccggt
     1261 ccgcgggcgg gcggcggctc accagtgcga ggccccgcgc tggcggcgtc gcgcgaggtc
     1321 gagcgccagc gcgagcgcgg cgtgcaggct gccgggatcg gccttgcgcg tgccggccag
     1381 gtcgagcgcc gtgccgtggt ccaccgacgt gcgcacgatc ggcaggccca gcgtgaagtt
     1441 gacggcgtgg ccgaagctcg cgtgcttgag caccggcagc ccctggtcgt ggaacatcgc
     1501 caccagcgcg tcgtactcgt cgagcttgcg cggcaggaac gccgtatcgg cgggcaacgg
     1561 cccgcgcagg tcgagcccgt ccgcgcgcag cgcacgcagc accggctcga tcacctcgat
     1621 ctcctcgcgt cccaggtggc cgccttcgcc cgcgtgcggg ttcaggccga gcaccgcgat
     1681 ccggggccgc cgcacgccaa acagcgcacg caggtcggcg tcgacgatgc gcagcacggc
     1741 ggcgaggcgc tcgggcttca atgcagcgct gaccttgcgc aatggcaggt gagtcgtcgc
     1801 gagcgccacg cgcatgccgc cgccgacgag catcatcacg gggagcggtg cgcccgtctg
     1861 ctgcgccagg tattcggtgt gaccggtgaa gcgcacgcca gcgtcgttga tgacggattt
     1921 ctgcaccggc gccgtgacga tggcatcgaa cgcgcccgcg gcgcagccgc cgatggcggc
     1981 atcgagcgtg tcgagcacgt agcgggcgtt ggcgggatcg agctggccgg gcgtgcaggg
     2041 cgccgcaagg ggcacgtgca gcagcgacac gccggcgcgg cggggagcgt gcaggtcgaa
     2101 cggtgccagc gccgcggcct tgcgcgcacg gccccgtcgc gagcgcgcga gcagcgagcc
     2161 gtcaccgatc aggacgatgt ggcagccgcg cggcaggtct tcgaggagtg cgctgcacag
     2221 gtccggcccg acgccggcgg gttcgccggt ggtcagcgcg agggtgcgca gcgcgggcgc
     2281 gcggcgcgtg ccggtgcggg gcttcacatc cggtattcga cgtaggcttc gtcgcgcagg
     2341 cggcgcagcc acatctcggt ctcttcctcg gcgcgcgcct cgcgcagctg gctgacgcag
     2401 cggttgcgca gcatgtcttc gctcgcgtcg tacgtgcggc gcccgagcac ctgcacgatg
     2461 tgccagccgt actgcgtgcg gaagggctcg ctgatctcgt tgatctgcag cgcgtcgatc
     2521 tgcttctgga actccggcac gaaggtgccg gggccggtcc agccgaggtc gcctccgttc
     2581 gcagcggagc ccgggtcttc ggaggtcacc gaggcgatgg cctcgaagct ttcgcctttc
     2641 aggatgcgct ggcggatctg ctccagcttc tgcttcgtgg tctggtcgtc ggcgatctcg
     2701 gtgggcttga gcaggatgtg gcgcgcgttg acctgctgca ccatcgccgg gccgccgccg
     2761 ccacgcactt cgagcacctt gaagatgtgc aggccgcttg gcgtgcggat gggctcggtg
     2821 acgtccccgg ccttcatgtt gggcaccagg tcggcgacga acgagggcag ctggccggcc
     2881 ttgcgccagc cgagcgcgcc gccttcgagc gccgtgccgg cgtcggagta cgtcacggcg
     2941 agctggccga agtcctcgtt cttgcgggca cgctcgtaga cg
//
LOCUS       DBNHBY010000760         2546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_58279, whole genome shotgun sequence.
ACCESSION   DBNHBY010000760 DBNHBY010000000
VERSION     DBNHBY010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_58279"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="AC16HB_17910"
     CDS             complement(<1..2054)
                     /locus_tag="AC16HB_17910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR5608720.1"
                     /translation="MHPAPLVAFVAGVLWLQRQSALPGTAAIALCALAAAAMFITARA
                     ARDALPRPGAVALHAAGAAALGFAWAASIASVRLADELRFADEGRDVRIVGVVSSLPA
                     QVERGTRFEFEVERVESADVHVPARISLAWYGANAAVRPAERWRFTVRLKRPHGVLNP
                     GGFDLEAWMIERNLRASGYVRDASGDDAPQRVDAVSWDFGALIDRGRHELRERLQQRL
                     QGERYGGVLIALILGDQRAIREEDWALFNRTGISHLVSISGLHITMIAGLAAAAASLA
                     WRRSRRLLALAPAQSAAALAAMLAALAYCLLAGWGVPAQRTFFMLAAVALALLARRGT
                     RAFATLAIAAAVVCLLDPWAAIAPGFWLSFGAVAAIFYVVTGRPATEIGWHAKLREAA
                     RIQCAVTLALVPLTIALFQQLSIVSPVANAIAIPLVSLVITPLALLGGFFVVLPEPLA
                     TLAVPCLAISHWLLAQLAAALQWSVALPWASLAVAAPPWWTLALALVAVAWLLAPRGW
                     PLRWIALAWFVPMFVWPPARPAAGELWVTALDVGQGMALLIESRAHALLYDTGPRYSA
                     ESDAGARVIAPYLRWRGLDRLDLLVVSHLDSDHSGGTASLLRGVPIDRVWTSVRADHS
                     MLKSVPNALRCSDGQSLALGALDLQVLRPRPDDYERALSTNAMSCVLEVRSGPHRVLL
                     TG"
     gene            complement(2056..>2546)
                     /locus_tag="AC16HB_17915"
     CDS             complement(2056..>2546)
                     /locus_tag="AC16HB_17915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5608721.1"
                     /translation="RERQQWFYAEQLKIARRFDLPVILHVRRSADDLLKFLRRVDLRG
                     GIAHAFNGSDDQAHRLLERGFRLGFGGAMTYDGSLRIRRFAATLPDDAWVLETDAPDI
                     PPQWLRGGPTLRNEPHELARIGATMAALRGLDAEGVAARNRANACAALPRLSALLAPV
                     SA"
BASE COUNT          426 a          926 c          891 g          303 t
ORIGIN      
        1 tcgccggtca gcaacacgcg atgcggcccg ctgcgcactt cgagcacgca gctcatcgcg
       61 ttggtcgaca gcgcgcgctc gtagtcgtca ggccgcggcc gcaagacctg caggtcgagc
      121 gcgccaagcg ccagcgactg cccgtcgctg cagcggagcg cgttcggcac cgatttcagc
      181 atcgagtggt ccgcgcgcac ggaggtccac acgcgatcga tcggcacgcc gcgcagcagt
      241 gatgcggtcc cgcccgagtg gtcgctgtcg aggtgcgaca ccacgagcag gtcgaggcga
      301 tcgaggccgc gccagcgcag gtacggggcg atcacgcgcg cgccggcgtc gctctccgcg
      361 gagtagcgcg gcccggtgtc gtacagcagc gcgtgcgcgc gcgactcgat cagcagcgcc
      421 atgccctgcc cgacatcgag cgcggtcacc cacaattcgc ccgcggcggg gcgcgccggc
      481 ggccagacga acatcggcac gaaccacgcc agcgcgatcc agcgcagcgg ccagccgcgc
      541 ggcgcgagca gccaggccac cgcgacgagc gcgagcgcca gtgtccacca tggcggcgcg
      601 gcgactgcga ggctcgccca cggcagcgcc acgctccact gcagcgcggc ggcaagctgc
      661 gcgagcagcc agtgcgaaat cgcaaggcag ggcaccgcaa gcgttgcgag cggttccggc
      721 agcacgacga agaacccgcc gagcagcgcc agcggtgtga tcaccaggct gaccagtgga
      781 atcgcgatcg cattggcgac cggcgacacg atcgacaact gctggaacag cgcgatcgtc
      841 aacggcacca gcgccagcgt gaccgcgcac tggatgcggg cggcttcgcg caacttcgcg
      901 tgccagccga tctcggtcgc tggccggccc gtgacgacgt agaagatcgc cgccacggcg
      961 ccgaatgaca gccagaagcc tggagcgatc gccgcccacg ggtcgagcag gcacacgaca
     1021 gccgccgcga tcgccagcgt ggcgaatgcg cgcgtgcccc ggcgcgcgag cagcgcgagc
     1081 gcgacggccg ccagcatgaa gaacgtgcgc tgcgccggca cgccccaacc ggcgagcagg
     1141 cagtacgcca gcgcggccag cattgccgcc agtgccgcgg cgctttgtgc tggcgccagc
     1201 gcaagcagcc ggcgtgagcg gcgccacgcg agacttgccg ctgctgccgc cagcccggcg
     1261 atcatcgtga tgtgcaggcc cgagatcgac accaggtgcg agattccggt gcggttgaac
     1321 agcgcccagt cctcttcgcg gatcgcgcgc tggtcgccga gaatcagtgc gatcagcacg
     1381 ccgccgtagc gctcgccctg cagacgctgc tgcagccgtt cgcgcaattc gtgccggccg
     1441 cggtcgatca gcgcgccgaa gtcccacgaa acggcatcga cccgctgcgg cgcgtcgtcg
     1501 cctgacgcat cgcgcacgta gccgctggcg cgcaggttgc gctcgatcat ccacgcctcg
     1561 agatcgaatc cgcccggatt cagcacacca tgcggacgct tcaaccgcac cgtgaagcgc
     1621 cagcgttcgg ccggccgcac ggctgcgttg gcgccgtacc acgcgagcga tatgcgtgcc
     1681 gggacgtgga catccgccga ttcgacgcgc tcgacctcga attcgaagcg cgtgccgcgc
     1741 tcgacctgcg ccggcagact cgatacgacg cccacgatgc gcacgtccct gccctcgtcg
     1801 gcgaagcgca gttcgtcggc aagacgcacg ctcgcgatcg acgcggccca tgcgaatcca
     1861 agcgcggccg cgcccgccgc gtgcagcgcg accgctccag gtcgaggcag agcgtcacgc
     1921 gcggcccgcg cggtgatgaa catcgcggca gcggccagcg cgcagagcgc aatggctgcc
     1981 gtgcccggca gtgcggactg gcgctgcagc cacagcaccc cggcgacgaa cgccaccagt
     2041 ggtgccggat gcatgtcagg cggaaacggg agcgagcagc gccgacagcc gcggcagggc
     2101 agcgcaggcg ttggcgcgat tgcgtgccgc gacgccctcc gcatcgagtc cgcgcagcgc
     2161 ggccatggtc gcaccgatgc gcgccagttc gtgcggctcg ttgcgcagcg tcgggccgcc
     2221 gcgcagccac tgcggcggga tgtccggcgc atcggtctcg agcacccagg cgtcgtccgg
     2281 cagcgtggcg gcgaagcggc ggatgcgcag cgacccgtcg taagtcatcg cgccgccgaa
     2341 gccaaggcgg aatccgcgct cgagcaaccg atgcgcctgg tcgtcgctgc cgttgaatgc
     2401 atgcgcgata cctccgcgga ggtcgacccg gcgcaggaac ttcagcaggt cgtcggccga
     2461 gcggcgtacg tgcaggatca ccggcaggtc gaagcggcgc gcgatcttca actgttctgc
     2521 atagaaccac tgctgacgct cgcgcg
//
LOCUS       DBNHBY010000761         2332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_938331, whole genome shotgun sequence.
ACCESSION   DBNHBY010000761 DBNHBY010000000
VERSION     DBNHBY010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_938331"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="trpC"
                     /locus_tag="AC16HB_17920"
     CDS             complement(<1..681)
                     /gene="trpC"
                     /locus_tag="AC16HB_17920"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR5608722.1"
                     /translation="MEDLLARILAVKRDEVARARVHEPLAALRARAEARRDVRPFEGA
                     LRAKITAGLPAVIAEVKKASPSKGVLRADFDPAAIAASYARHGAACLSVLTDAQFFQG
                     APEYLDQARQASALPALRKDFIVDEYQVAEARALGADCILLIVAALEAQQLRVLERAA
                     QGYGMDVLVEVHDAQELDVALTLDTQLIGINNRNLRTFEVSLATTTGLLPRIPSGKLV
                     ITESGILHP"
     gene            complement(681..1712)
                     /gene="trpD"
                     /locus_tag="AC16HB_17925"
     CDS             complement(681..1712)
                     /gene="trpD"
                     /locus_tag="AC16HB_17925"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5608723.1"
                     /translation="MAITPTEALVRCIEHREIFHDEMLALWRKLMSGDLTPVQIAAVT
                     MGLRVKKETIGEIAAAAQIMREFATKVPVDDHRDLVDIVGTGGDAAHTFNISTAAMFV
                     AAAAGGRVAKHGGRSVSSSSGSADVLEALGANINLSPEKVATCLAETGIGFMFAPNHH
                     AAMKHAAPVRKELGVRTIFNILGPLTNPAGAPNQLMGVFHPDLVGIQVRVLQQLGSRH
                     VLVVYGKDGMDEVSLGAATMVGELKNGDVREYEIHPEDFGMSMVSNRGLKVSNATESR
                     AMVLEALENVQGTPREIVTLNAGVALYAADVAGSIDDGIRMARETIASGAARRKLDQF
                     VAASQKLAA"
     gene            complement(1727..2293)
                     /locus_tag="AC16HB_17930"
     CDS             complement(1727..2293)
                     /locus_tag="AC16HB_17930"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR5608724.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEEVRVARNDEITLDEIAALKP
                     DRICISPGPCTPAEAGISLDVLNRFGGTMPILGVCLGHQAIGHAFGGRVIRAQTLMHG
                     KTAPVEHTGAGVFKGLPTPFTATRYHSLAVERASLPDCLEITAWTADGEIMGLRHKSL
                     AVEGVQFHPESIATEHGHELLKNFLRTN"
BASE COUNT          354 a          827 c          756 g          395 t
ORIGIN      
        1 cggatgcagg atgccgctct cggtgatcac gagcttgccg gacggaatcc gcggcaaaag
       61 acccgtcgtc gtcgcgagcg acacctcgaa cgtgcgcagg ttgcggttgt tgataccgat
      121 gagctgcgtg tcgagcgtca gcgcgacgtc gagctcctgc gcatcgtgca cctcgaccag
      181 cacgtccatg ccgtagccct gcgccgcacg ttcgagcacg cgcaactgtt gcgcctcgag
      241 cgcggcgacg atcagaagaa tgcagtcggc gccgagcgcg cgcgcctccg cgacctgata
      301 ctcgtcgacg atgaagtcct tgcgcagcgc cgggagcgca ctcgcctggc gcgcctgatc
      361 gagatactcc ggcgcgccct ggaagaactg cgcgtcggtc agcacggaca ggcacgccgc
      421 accgtggcgc gcgtagctgg cggcgatcgc ggcgggatcg aagtcggcgc gcagcacgcc
      481 cttcgacggc gacgccttct tgacctccgc gatcacggcc ggcaacccgg ccgtgatctt
      541 cgcgcgcagc gcgccttcga acggacgcac gtcgcggcgc gcctcggccc gcgcgcgcag
      601 cgccgcgagc ggctcgtgca cccgcgccct ggcaacctcg tcgcgcttga ccgcgaggat
      661 gcgcgccagt aagtcttcca tcaggccgcc agcttctgcg aggccgcgac gaactggtcg
      721 agcttgcgtc gtgccgcacc gctggcgatc gtttcgcgcg ccatccggat gccgtcgtcg
      781 atcgagcccg cgacatcggc cgcgtagagc gccacgccgg cgttcagcgt cacgatctcg
      841 cgcggcgtgc cctgcacgtt ctccagtgcc tcgagcacca tcgcgcgcga ctcggtcgca
      901 ttgctcacct tcagcccgcg gttcgacacc atgctcatgc cgaagtcctc gggatggatc
      961 tcgtactcgc gcacgtcgcc gttcttcagc tcgccgacca tggtggccgc gccgagcgac
     1021 acctcgtcca tgccgtcctt gccgtagacg accagcacgt gccggctgcc gagctgctgc
     1081 agcacgcgca cctggatgcc gacgagatcc ggatggaaga ctcccatcag ctggttcggc
     1141 gcgccggccg ggttggtcaa tgggcccagg atgttgaaga tcgtgcgcac gcccagttcc
     1201 ttgcgcaccg gtgcagcgtg cttcatcgcc gcgtgatgat tgggcgcgaa catgaagccg
     1261 atgccggtct ccgcgaggca ggtcgccacc ttctctggcg acaggttgat gttggcgccg
     1321 agcgcctcca gcacgtcggc gctgcccgaa ctcgacgaca cgctgcggcc gccgtgcttg
     1381 gccacgcgcc cgcctgccgc cgcggcgacg aacatagcgg cggtcgagat gttgaacgtg
     1441 tgcgcggcat cgccgccggt gccaacgatg tcgaccaggt ccctgtgatc gtccaccggc
     1501 accttggtcg cgaactcgcg catgatctgc gcggccgcgg cgatctcgcc gatcgtctcc
     1561 ttcttcaccc gcaggcccat cgtgaccgcg gcgatctgca ccggggtcaa atcgccgctc
     1621 atcagcttgc gccacagcgc cagcatctcg tcgtggaaga tctcgcggtg ctcgatgcag
     1681 cggaccagcg cctcggtcgg cgtgatggcc atgtggctcc tcctcgtcag ttcgtgcgca
     1741 ggaaattctt caagagctcg tgaccgtgct cggttgcgat cgattccggg tggaactgca
     1801 cgccctcgac cgcgagcgat ttgtgccgca accccatgat ctcgccgtcc gcggtccacg
     1861 cggtgatctc caggcagtcc ggcagcgacg cgcgctcgac cgccagcgag tggtagcgcg
     1921 tcgcggtgaa cggtgtcggc agccccttga acacgcccgc gccggtgtgc tcgaccggcg
     1981 ccgtcttgcc gtgcatcagc gtctgcgcgc ggatcacccg tccgccgaac gcgtggccga
     2041 tcgcctggtg tccgaggcag acgccgagga tcggcatcgt gccgccgaag cggttcagga
     2101 cgtcgagcga gattcccgcc tcggccggcg tgcacggccc gggcgaaatg cagatgcggt
     2161 ccggtttcag cgccgcgatc tcgtcgagcg tgatctcgtc gttacgagcg acgcgcacct
     2221 cctcgcccaa ctcgccgaag tactgcacca ggttgtaggt gaaggaatcg tagttgtcga
     2281 tcatcagcag cataagttgc ccaaccggtt gatttactcg tccaggcaca gc
//
LOCUS       DBNHBY010000762         4257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_666781, whole genome shotgun sequence.
ACCESSION   DBNHBY010000762 DBNHBY010000000
VERSION     DBNHBY010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_666781"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..763)
                     /gene="phnC"
                     /locus_tag="AC16HB_17935"
     CDS             complement(5..763)
                     /gene="phnC"
                     /locus_tag="AC16HB_17935"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR5608725.1"
                     /translation="MSAALVLRGLVKEYVASRRVLDGIDLEFTPSGLTAIIGASGTGK
                     STLLRCVNRLVDPTAGAILFDRRDLARLQGRELREARREIGMVFQEYNLVERLTVMEN
                     LLSGRLGYVPALKAWLRRFPPADIERAFDLLDQVGLTGFANQRADALSGGQRQRVGIA
                     RAVMQAPKLLLADEPTSSLDPRTSVEIMELMRALGERHAIPVIVNMHDVELARRFADR
                     VIGMAGGKVVFDDAPGALSEEALTRIYGGRSWLQ"
     gene            complement(760..1764)
                     /gene="phnD"
                     /locus_tag="AC16HB_17940"
     CDS             complement(760..1764)
                     /gene="phnD"
                     /locus_tag="AC16HB_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5608726.1"
                     /translation="MRVGALAATLMLAASPLLAQDACRHRGDLDTPYCDEDGDLLADM
                     PKDPAKQKNPETLFFTNSPLDDPAVFNKLMQPFIDHLANCAGKRVRYYDVFSSAAAIE
                     AMRAGRMHIGTMSSGDTAFAVNVAGAIPLGIRGDASGPRGYQLWLIVRRDSPLQKLAD
                     LKGKRVAHTTPSSNSGNLAPRALFPAEGLVPDKDYKVLFSGKHENSISGVAAGDYDAA
                     PIAHDILVRMAERGIVKMEEFRIIWKSRSFPPGGLSMAHDLAPALQGKIRGCTYNFRY
                     PPEMQKGFQGADRWWPIDYKKDWEIVRRVAEVSGQGFTRAAFEKEKARAESAQPAARK
                     "
     gene            complement(1769..2791)
                     /gene="phnD"
                     /locus_tag="AC16HB_17945"
     CDS             complement(1769..2791)
                     /gene="phnD"
                     /locus_tag="AC16HB_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5608727.1"
                     /translation="MIHIARALALIALATLSFSAAAQECKHRGDLDPMYCDDNGDLVA
                     DTPKDPKKLKVPNTLVFTYTPVEDPAVYEKIFKPFTDYLAQCTGKRVVFYQVQSNAAE
                     IESMRSGRLHVGGFSTGPTAFAVNIAGAVPFAIKGYEKDFQGYNLIVIVKKDSPYQKL
                     TDLKGKKFAHTSPSSNSGHMAPMALFPKEGLAPDKDYKILFSGKHDQSVMGVNSGDYD
                     AAAVASDVFHRMATRGQIKEENFRIIYRSAKFPTSSFAYAHDLEPAFRDKMVKCFYDY
                     RYTDDMKKAFDGADRFYPINFKEHWEVVRQVAEAGGEKFNRDAYEKESKREEEARRKA
                     AEEKKK"
     gene            2896..3861
                     /locus_tag="AC16HB_17950"
     CDS             2896..3861
                     /locus_tag="AC16HB_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5608728.1"
                     /translation="MQGITRRAFLACALALPAAAVQAQPGKTVRILVGFPPGGGTDAI
                     ARILADRLTDELGTTVIVDNRPGAGGQIAAQQLKAAAPDGTTYFISHDHTISILPLVL
                     KDPGYDPARDFVPVAGFATFVNALALSAGTPARTFREYVAQVKAQGGKGTIGVPAPAS
                     VPEFLVKVIGEKYGLDLAAAPYRGSAPRIADMLGNQIAAGVGSVPDFVENHRAGKLRV
                     VAVLGAERQAALPDVPTLAELGLTGFEAVPYYGFFAPAGTPQAALDRFSAALARVIAL
                     PDVRERLIALGLNVEFMPQKDLARREHAYSATWARIVKASGFQPR"
     gene            complement(3833..>4257)
                     /locus_tag="AC16HB_17955"
     CDS             complement(3833..>4257)
                     /locus_tag="AC16HB_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608729.1"
                     /translation="RLGRAWHALLERGERALDAIAREHALTREQAGLVTAAAARVRQR
                     RPQFFGASAEAELELVDAGGELLRIDRLIETDDALWIVDFKWRITDAERRQYENQVRR
                     YAEVLQAIRCDKPIRTALIDADGELIAVEPIEAGIRLP"
BASE COUNT          647 a         1458 c         1393 g          759 t
ORIGIN      
        1 gcgctcactg cagccacgac ctgccgccgt agattcgcgt gagcgcctcc tcgctcagcg
       61 ctcccggcgc atcgtcgaac acgaccttgc cgcccgccat gccgatcacg cggtcggcga
      121 aacgacgcgc gagttcgacg tcgtgcatgt tgacgatcac cggaatcgcg tgccgctcgc
      181 cgagcgcgcg catcagttcc atgatctcca ccgaggtcct cgggtcgagc gacgacgtcg
      241 gctcgtcggc gagcagcagc ttgggcgcct gcatcaccgc gcgcgcgatg cccacgcgct
      301 ggcgctggcc gcccgacagc gcgtcggcgc gctgattggc aaagccggtg aggcccacct
      361 gatcgagcag gtcgaatgcg cgctcgatgt cggccggcgg aaagcgccgc agccacgcct
      421 tgagcgcggg cacgtagccg agtcgccccg acagcaggtt ttccatcacg gtcaggcgct
      481 cgaccaggtt gtattcctgg aacaccatgc cgatttcgcg ccgcgcctcg cgcagttcgc
      541 gaccctgcag gcgcgcgagg tcgcgacggt cgaacaggat cgcgcccgcc gtcggatcga
      601 cgaggcgatt cacgcagcgc aacagtgtcg acttgccggt gcccgacgcg ccgatgatcg
      661 cggtcaaccc gctcggtgtg aactcgaggt cgatgccgtc cagcacgcgg cggctagcga
      721 cgtactcctt gacgaggccg cgcaggacga gcgcggcgct catttccttg ccgcaggctg
      781 ggcggattca gcgcgcgcct tctctttctc gaacgccgcg cgcgtaaatc cctgcccgct
      841 cacctcggcc accctgcgca cgatctccca gtccttcttg taatcgatcg gccaccagcg
      901 gtctgcgccc tggaatccct tctgcatctc gggcggatag cggaagttgt acgtgcaccc
      961 gcggatcttg ccctgcagcg ctggtgccag gtcatgcgcc atcgacaggc cgccgggcgg
     1021 gaagctgcgg ctcttccaga tgatgcggaa ctcctccatc ttcacgatcc cgcgctcggc
     1081 catccgcacg aggatgtcgt gcgcgatcgg tgccgcgtcg tagtcgccgg cggcgacgcc
     1141 ggagatcgag ttctcgtgct tgccggagaa cagcaccttg tagtccttgt caggcacgag
     1201 cccttcggcc gggaacagcg cgcgcggagc gaggttgccg gagttcgacg acggggtcgt
     1261 gtgcgcgacg cgcttgccct tgaggtcggc gagcttctgc aacgggctgt ccctgcggac
     1321 gatcagccag agctggtagc cccgcgggcc gctcgcgtcg ccgcggattc cgagcggaat
     1381 cgcgccggcg acgttcaccg cgaacgcggt gtcgccggac gacatcgtcc cgatatgcat
     1441 gcggcctgcg cgcatcgcct cgatcgccgc cgcgctcgaa aacacgtcgt agtagcgcac
     1501 gcgcttgccg gcgcagttcg ccaggtggtc gatgaacggc tgcatcagct tgttgaacac
     1561 cgccgggtca tcgagcggcg agttggtgaa gaacagcgtc tcggggttct tctgcttcgc
     1621 ggggtccttc ggcatgtcgg ccagcaggtc gccgtcctcg tcgcagtacg gcgtgtcgag
     1681 gtcgccgcga tgccggcagg cgtcctgagc cagcagcggc gacgccgcca gcatcagcgt
     1741 ggccgccaga gcaccgacgc gcattgccct atttcttctt ctcttccgcc gccttgcgcc
     1801 gcgcctcttc ctcgcgcttg ctttccttct cgtacgcgtc gcggttgaac ttctcgccgc
     1861 ccgcctcggc cacctggcgc acgacttccc agtgctcctt gaagttgatc gggtagaacc
     1921 gatcggcgcc gtcgaacgcc ttcttcatgt cgtcggtgta gcggtagtcg tagaagcact
     1981 tgaccatctt gtcgcggaac gccggctcga ggtcgtgcgc ataggcgaac gacgaggtcg
     2041 ggaacttcgc gctcctgtag atgatgcgga agttctcctc cttgatctgt ccgcgcgtgg
     2101 ccatccggtg gaacacgtcg gaggcgaccg ccgcggcgtc gtagtcgccg gagttcacgc
     2161 ccatcacgga ctggtcgtgc ttgcctgaga acaggatctt gtagtccttg tccggcgcca
     2221 gcccctcctt cgggaacagc gccatcggcg ccatgtgccc cgaattcgac gacggcgacg
     2281 tgtgcgcgaa tttcttgccc ttcaggtcgg tgagcttctg gtacgggctg tccttcttca
     2341 cgatcacgat caggttgtag ccctggaaat ccttctcgta gcccttgatc gcaaacggga
     2401 ccgcgccggc gatattgacc gcaaaggcag tcggtccggt cgagaacccg ccgacatgca
     2461 gacggcccga gcgcatcgac tcgatctcgg cggcgttgga ctgcacctgg tagaagacca
     2521 cgcgcttgcc ggtgcactgc gcgaggtaat cggtgaatgg cttgaagatc ttctcgtaca
     2581 ccgccggatc ctccaccggc gtgtaggtga acaccagcgt gttcggaacc ttcagcttct
     2641 tcggatcctt gggcgtgtcg gcaacgagat cgccgttgtc gtcgcaatac atcggatcca
     2701 ggtcgccgcg gtgcttgcat tcctgggccg cagcggaaaa cgagagagta gcgagcgcaa
     2761 tcagcgccag cgcacgagcg atgtgaatca tggtcggtct cctgttcttg ttggcggcgc
     2821 gaattctgac agaagccccg acgaattcga tcatcggcgc cggcataatc ggctgcgggt
     2881 ttcaggggag gtcgtatgca agggatcacc cgccgcgcct tcctcgcctg cgcgctggcg
     2941 ctgcctgccg cggcggtgca ggcgcagccg ggcaagacgg tacgcatcct ggtcggcttc
     3001 cctcccggcg gcggcaccga cgcgattgca cgcatcctcg ccgatcgcct gacggacgag
     3061 ctcggtacga cggtgatcgt cgacaaccgg ccaggcgcgg gcggtcagat cgcggcacag
     3121 cagctgaagg cggccgcgcc cgacggcacg acgtacttca tctcgcacga tcacacgatc
     3181 tcgatcctgc cgctggtgct gaaggatccg ggctatgacc cggcgcgcga tttcgttccg
     3241 gtggccggat tcgccacgtt cgttaacgcg ctcgcgctgt cggccggcac gccggccagg
     3301 acgttccgcg aatatgtcgc gcaggtgaaa gcgcagggcg gcaaaggcac gatcggcgtg
     3361 ccggcgccgg cgtcggtccc cgagttcctc gtcaaggtga tcggcgagaa gtacgggctc
     3421 gatctcgccg ccgcgccgta tcgcggcagc gcgccgagga tcgccgacat gctcggaaac
     3481 cagattgccg cgggcgtggg ctccgtcccc gatttcgtcg agaaccaccg ggccggcaag
     3541 ctgcgcgtgg tcgcggtgct cggggccgaa cggcaggccg ccctgcccga cgtgccgacg
     3601 ttagccgaac tggggttgac gggcttcgag gccgtcccgt actacggctt cttcgcaccg
     3661 gccggcacgc cgcaggcggc gctcgatcgg ttttccgccg cgctcgcaag ggtgatcgca
     3721 ttgccggacg tgcgcgagcg tctgatcgcg ctcggcctga acgtcgaatt catgccgcag
     3781 aaggacctgg cgcggcgcga acacgcgtac tcagccactt gggcacgcat cgtcaaggca
     3841 agcggattcc agcctcgata ggctccactg cgatcagttc gccgtccgcg tcgatcagcg
     3901 ccgtccggat cggtttgtcg cagcggatcg cctgcagcac ttccgcatag cgccgcacct
     3961 ggttctcgta ctgccgccgc tcggcgtcgg tgatgcgcca cttgaagtcg acgatccaca
     4021 gcgcgtcgtc ggtctcgatc agccggtcga tgcgcagcag ctcgccgcca gcgtccacca
     4081 attcgagttc cgcttccgca ctggcgccga agaactgcgg acggcgttgg cgcacgcgcg
     4141 cggcggcggc ggtcacgagg cccgcctgct cgcgcgtcag cgcgtgctcg cgcgcgattg
     4201 cgtcgagcgc gcgctcgccc cgctccagca gtgcgtgcca cgcgcgaccg agccgtt
//
LOCUS       DBNHBY010000763         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_396911, whole genome shotgun sequence.
ACCESSION   DBNHBY010000763 DBNHBY010000000
VERSION     DBNHBY010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_396911"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="hrpA"
                     /locus_tag="AC16HB_17960"
     CDS             complement(<1..1520)
                     /gene="hrpA"
                     /locus_tag="AC16HB_17960"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR5608730.1"
                     /translation="MSQPPRQLAPRTLPAIPFPADLPIAARRDEIARAIEAHPVVIVC
                     GETGSGKTTQLPKICLALGRGARKLIGHTQPRRIAASSIARRIAQELDSPLGSVVGFK
                     VRFTDQTRPGASIKLMTDGILLAESQSDPLLSAYDTIIIDEAHERSLNIDFLLGYLKQ
                     LLPRRPDLKLIITSATIDAERFAQHFGTKDAPAPVVNVSGRLYPVEVRYRPVKDEDDA
                     DDRTLMDAIVDAVDECALTGRGDVLVFLPGEREIREAAEALRKHHPPGTEILPLYARL
                     SAEEQDRVFKPGGARRIVLATNVAETSLTVPGIRYVVDTGLARVKRYSYRNKVEQLQV
                     EPVSQAAANQRAGRCGRTSNGICIRLYGEADFNGRPRFTDPEIVRSSLASVILRMKSL
                     RLGDVRAFPFVEPPPARAIADGYDLLAELNAVDEEDDLTPIGAELARLPLDPRIGRML
                     LAARDNHALAEILIIAAALSVQDPRERPLESQQAADQAHRRHADERSDFLSFVKLW"
     gene            1556..>1807
                     /locus_tag="AC16HB_17965"
     CDS             1556..>1807
                     /locus_tag="AC16HB_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5608731.1"
                     /translation="MNDSTPESQLAQSGQAQFVHWLREVAPYVHAHRGRTFVVGFGGE
                     LIDAGRLTALVQDLSLLHAMGIRLVIVHGSRPQVQAQLAL"
BASE COUNT          270 a          616 c          637 g          284 t
ORIGIN      
        1 ttccacagct tcacgaacga caggaagtcg gacctctcat cggcgtgccg gcggtgcgcc
       61 tgatcagccg cctgttgcga ttccagcggc cgctcgcgcg gatcctgcac cgacagcgcc
      121 gcggcaatga tcagtatctc ggccagcgcg tggttgtcgc gcgccgcgag cagcatgcga
      181 ccgatgcgcg ggtcgagcgg cagccgcgcg agttccgcgc cgatcggcgt gaggtcatct
      241 tcttcgtcga ctgcattcag ttccgccagc aggtcgtagc cgtcggcgat cgcgcgcgcc
      301 ggcggcggct cgacgaacgg aaatgcgcgc acgtcgccga gtcgcagcga cttcatccgc
      361 aggatcacgc ttgcgagcga cgaacgcacg atctccggat cggtgaagcg cggccggccg
      421 ttgaagtccg cctcgccgta caggcggatg cagatgccgt tgctcgtccg gccacagcgc
      481 ccagcccgct ggttggccgc cgcctgcgaa accggctcga cctgcagttg ctcgaccttg
      541 ttgcgatacg agtagcgctt gacgcgcgcg agccccgtgt cgacgacgta gcggatgccg
      601 ggcaccgtga gcgacgtctc tgccacgttg gtcgcgagca cgatgcgccg cgcgccgccg
      661 ggcttgaaca cgcggtcctg ttcctcggcc gacagccgcg cgtagagcgg caggatctcg
      721 gtgcccggcg gatggtgctt gcgcagcgcc tcggcggctt cgcggatctc ccgctcgccg
      781 ggcaggaaca ccagcacgtc gccgcggccc gtcaacgcgc actcgtcgac ggcgtcgacg
      841 atcgcgtcca tcagcgtacg gtcgtccgcg tcgtcctcgt ccttgaccgg cctgtaccgg
      901 acctcgaccg ggtacaaccg acccgacacg ttgaccaccg gcgcgggcgc gtccttcgtg
      961 ccgaagtgct gcgcgaagcg ctccgcgtcg atcgtcgccg aggtgatgat cagcttcaga
     1021 tccgggcgac gcggcagaag ctgcttcagg tacccgagca gaaagtcgat gttcagcgac
     1081 cgctcgtgcg cctcgtcgat gatgatcgtg tcgtacgcgg acagcagcgg gtccgactgc
     1141 gactcggcga gcaggatgcc gtcggtcatc agcttgatcg aggcgcccgg ccgcgtctgg
     1201 tcggtgaagc ggaccttgaa gccgaccacc gagcccagcg gcgagtccag ttcctgcgcg
     1261 atgcggcgcg caatcgacga cgcggcgatg cgccgcggct gcgtgtggcc gatgagcttc
     1321 cgcgcgccgc ggccgagcgc gaggcagatc ttcggcaact gcgtggtctt gcccgagccg
     1381 gtctcgccgc agacgatcac caccgggtgc gcctcgatcg cgcgcgcgat ctcgtcgcgc
     1441 cgagcggcga tcggcaggtc tgcgggaaac ggaatcgcgg gcagcgtgcg cggcgcgagt
     1501 tgacggggcg gctgcgacat cgatcggaga tatggcgcgc aactatactg cgcgcatgaa
     1561 cgacagcacg ccggaaagcc agctcgcaca gtcgggccag gcgcagttcg tccactggct
     1621 gcgcgaggtg gcgccgtacg tgcatgcgca tcgcggcagg acgttcgtgg tcggctttgg
     1681 cggcgagctg atcgacgccg gacgcctgac cgcgctggtg caggacctgt cgctgctgca
     1741 cgcgatgggg atcaggctcg tgatcgtgca cggctcgcgg ccgcaggtgc aggcgcagct
     1801 cgcgctg
//
LOCUS       DBNHBY010000764         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_58751, whole genome shotgun sequence.
ACCESSION   DBNHBY010000764 DBNHBY010000000
VERSION     DBNHBY010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_58751"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="AC16HB_17970"
     CDS             <1..896
                     /locus_tag="AC16HB_17970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608732.1"
                     /translation="LAAFHWITRPLRELTADVRRFGADASHAVAPPSVPARIAHGRDE
                     LATLRMVFAQMTQRIGEQWRELTTQDQQRRELVANISHDLRTPLMSLHGFLETLRVKA
                     DTLSAGERQRYLDVALGQSRKVGRLAQELFELARLEYGVVKLDRERFSVADLVQDVFQ
                     KFELAAESRKQRLLAVIAPDLPVVSADVGLIERVLTNLLDNALRHTPAGGEIEVQLRR
                     AGDAIDVMVSDSGPGIPEALQKTLFVRPSFQSGGRERSGGLGLVIVKRILQLHGSDIR
                     IVSGAQRGAAFQFQLQVTAAA"
     gene            complement(1041..>1966)
                     /locus_tag="AC16HB_17975"
     CDS             complement(1041..>1966)
                     /locus_tag="AC16HB_17975"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR5608733.1"
                     /translation="APRIEVRRGAEMEVPHVLMLIDDPAGTVIEPIGAERDRLTKLYE
                     ADLMLGGGHVAGYAVDAARGERAARALRALMDPLEFATHYAVPDGTPVMLFASGDGNH
                     SLATAKAYWDSIKASVGMNHPSRFALVEVENIHDPALEFASIHRLLFGVSADVRQALA
                     EAFGSRLSCTDVSSAAAMRARVQAAPGDRHAAGLIGPGERFSVIEIAAPESTLAVGTL
                     QPFVDRFIERGGATHVDYVHGDEALERLAIKGGCVGIHLAPVSKHDLLRMVVREGPTP
                     RKTFSMGEAHEKRYYIEARRIRAPGDAASAR"
BASE COUNT          299 a          678 c          684 g          305 t
ORIGIN      
        1 ggctcgcggc ttttcactgg atcacgcgtc cgctgcggga actcaccgcg gatgtgcgcc
       61 gcttcggtgc cgacgcgtcg cacgccgtcg cgccaccctc ggtgccggcg cgcatcgcgc
      121 acggccgcga cgagctggcg acgctgcgca tggtcttcgc gcagatgacg caacggatcg
      181 gcgagcagtg gcgtgaactg acaacgcagg accagcagcg gcgcgaactg gtggccaaca
      241 tctcgcacga cttgcgcacg ccactgatgt cgctgcacgg cttcctcgaa acgctgcgcg
      301 tgaaggccga cacgctgtct gccggcgagc gtcagcgcta tctcgacgtc gcgctcggcc
      361 agagccgcaa ggtcggccgc ctcgcgcagg agttgttcga actggcgcgc ctcgaatacg
      421 gcgtcgtgaa gctcgatcgg gagcgcttct cggtggccga cctggtgcag gacgtgttcc
      481 agaagttcga actcgcggcc gagtcacgca agcagcgcct gctcgccgtc atcgcgcccg
      541 atcttccggt ggtgagcgcg gacgtgggcc tgatcgagcg cgtgctgacc aacctgctcg
      601 acaacgcgct ccgccacacg ccggccggcg gcgagatcga ggtgcagctg cgccgcgccg
      661 gcgacgcgat cgacgtgatg gtcagcgact cggggccggg aattccggag gcactgcaga
      721 agacgctatt cgtccggccg tcgttccaga gcggcggacg cgaacgcagc ggcggcctgg
      781 ggctggtgat cgtcaagcgc atcctgcaac tgcacggcag cgacatacgg atcgtgtcgg
      841 gcgcgcagcg tggcgcggcg tttcagtttc agctgcaggt gacagccgcc gcataggcag
      901 cgcgcgccat gtctcggacg aggcggccgt cgctgcgcag cccgtcgtca attccgggcc
      961 gggcgcccgg gctcggtgat tgacccgcct gcccggtagc gcgcaagaaa ccgggcacgg
     1021 tcgattcgga gatgcgcgcg tcagcgcgct gacgcggcgt cccccggtgc gcggatgcgc
     1081 cgcgcctcga tgtagtagcg cttctcgtga gcctcgccca tcgagaacgt cttgcgcggc
     1141 gtggggcctt cgcgcaccac catgcgcagc aggtcgtgtt tgctcactgg cgcgagatgg
     1201 atgccgacgc agccgccttt gattgccaga cgctcgagcg cctcgtcgcc gtgcacgtag
     1261 tcgacgtgcg tcgcgccgcc gcgctcgatg aagcgatcga cgaacggctg caacgtaccc
     1321 accgcgagcg tggactctgg tgcggcgatc tcgatcacgc tgaagcgctc gcctggtccg
     1381 atcaatccgg cggcgtggcg gtcgcccggc gcggcctgca cgcgcgcgcg catcgcggcc
     1441 gcggacgaca catcggtaca ggacagtcga ctgccgaacg cctcggccag cgcctggcgc
     1501 acgtcagccg acacgccgaa gagcagccgg tggatcgacg cgaactccaa cgcaggatcg
     1561 tggatgttct cgacttcgac cagcgcgaag cggctcgggt ggttcatacc caccgacgcc
     1621 ttgatgctgt cccagtacgc cttggcggtg gccagcgagt gattgccgtc gccggaggca
     1681 aacagcatca ccggcgttcc gtctggtacg gcgtagtgcg tggcaaactc gagcggatcc
     1741 atcagggcgc gcagcgcccg tgcggcgcgc tcgccgcggg ccgcgtcgac cgcgtagccg
     1801 gcgacgtgcc cgccgccgag catcaggtcg gcttcgtaga gcttcgtcag tcgatcgcgt
     1861 tccgcgccaa tgggttcgat caccgtgcct gccgggtcat cgatcagcat caggacgtgc
     1921 ggcacttcca tctcggcgcc gcgccgcacc tcgatgcgcg gggcca
//
LOCUS       DBNHBY010000765         4580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_938802, whole genome shotgun sequence.
ACCESSION   DBNHBY010000765 DBNHBY010000000
VERSION     DBNHBY010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_938802"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="AC16HB_17980"
     CDS             <1..1595
                     /locus_tag="AC16HB_17980"
                     /inference="COORDINATES: protein motif:HMM:NF018662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HIR5608734.1"
                     /translation="IGGRVAGALDPAFSNPDFNFLNGDDGNLNYKKGDITSLALKGTH
                     ELGLKWGNGWKSLARATWAYDAKVDDTRRTDLSGDAKNLAQHNITLLDLWLSKDTKLI
                     NDRPATVKLGNQVISWGEDVFIVGGVNSINALDLRKYHTPGTQLKEIFRPAPMLYLNT
                     GLTEGLNLEGYYQFTWNAFQFDPVGTFFSAADVVGKGQRPAFIPSSLLTPFGLCTPPT
                     PCGDIGDKIQPGINVIPFEGDKKPPAGRQCGLALRGKPRGMDTEFALYYIRYHDKLPF
                     TTLFTDLNNPTYANANVLGAGYFNEFGKDKDLFGVSFNTKLGPVAVGGELSYRPRDSV
                     AIDPSVPATGPYSIFDPAPGPTKTVDPNGRITVRGFVNEKKFQAHLTGFYFIEVNSPM
                     GALMKGLGAAEGYVLAEAAVTRYPDLDPSKIPYLIFPSYEIPTKTSAGYVIEVGLTYP
                     HAFGSGVNLTPQIDFTHDVTGISPNTLPFVKGRKSFFFGVNYDIDSVWRGQIGVSRFF
                     GGDLTNAIRDRSFFSASVSYSF"
     gene            1624..4020
                     /locus_tag="AC16HB_17985"
     CDS             1624..4020
                     /locus_tag="AC16HB_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608735.1"
                     /translation="MERGTVGNAVGPMIELVVRRLEAVIFRHRVATLALLALLTVVLG
                     VSALQLRMEAGFDKQLPTGHEYTDTFFQYRDQVFGANRLMVAVARTKGDVWNVAALRK
                     LNDVTEAVLYMPGIDRRTVSSLWTPNTRVLQITEEGFKGMDVIGGNVTAENLTEAQVA
                     RIRDNAIQGGYVGSLVANDATATMVIADLLDYDPKTRAKTDYIELGRRIETHLRAKFE
                     DADTKIQVIGFAKQISDIADGAASVLRFFALAFVLTALAVYWYSRSVAFTVAAMLCSL
                     VSLVWQFGTLHLLGYGLDPLAVLVPFLVFAIGVSHGVQQVNYISRSICGGMTGADAAR
                     SSFRGLLVPGTMALVTAFVGFATLYLVPIPMIRELSLTASIGVAYKIITNLVMLPVVA
                     SFFHFGPDYVKRVTALRLEREGWMKRLDRVAERHVAFAVTGAAVVLFGLAVWQSSDRH
                     IGYLKPGAPELREDSRYNRDAVAIVEKFGLGLDVFTLVLEMPPDGCYEFARMSYVDDL
                     SWVMTNVPGVIGVASLSALVKIGASGFNEGNPKWQALPRDARSMASFIGLAGEGSGLY
                     DPRCSVLPINVSLADHKAVTLRAVVDAAKRYRQDNVNDTVKVRLASGNAGVQAATNEV
                     LEHTELPMMLYVYVTILLLVLITYRDWRAMIACCVPLTLATFLGYWFMKVLDIGLTVA
                     TLPVMVLAVGIGVDYAFYIYNRLQTYLADGMPIEPAFKNALLETGNATIFTAITLSIG
                     VATWGFSDLKFQADMGLLLAFMFMVNMVMAITLLPALAVVIDALIPRRGPVKRPLMAH
                     "
     gene            4022..>4580
                     /locus_tag="AC16HB_17990"
     CDS             4022..>4580
                     /locus_tag="AC16HB_17990"
                     /inference="COORDINATES: protein motif:HMM:NF026220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5608736.1"
                     /translation="MAPRLLALCAVLLIAVGALVHAADPAPAAASAAAPGGGNARPSN
                     LAVSNLLLAGTRAGERLVVVGEYGHVLLSDDDGANWRQAKLVPTRTTLTAVTFIDGRT
                     GWAVGHGGIVLGTRDGGENWTLLSGRIEGKDALFSVWFRDEKHGLVVGPYGFAARSDD
                     GGATWKSFEIAEGELGERHLNQIVAR"
BASE COUNT          751 a         1502 c         1542 g          785 t
ORIGIN      
        1 cgatcggcgg ccgcgtcgct ggcgcgctcg acccggcatt ctcgaacccc gacttcaact
       61 tcctgaacgg cgacgacggc aacctgaact acaagaaggg cgacatcacg tcgctggcgc
      121 tgaaaggcac gcacgaactc gggctgaagt ggggcaacgg ctggaagtcg ctcgctcgcg
      181 cgacctgggc gtacgacgcc aaggtcgacg acacgcgccg caccgatctg tcgggcgacg
      241 cgaagaacct ggcccagcac aacatcacgc tgctcgactt gtggctgtcg aaggacacca
      301 agctcatcaa cgaccggccg gcaacggtca agctcggcaa ccaggtgatc agctggggcg
      361 aggacgtgtt catcgtcggc ggcgtcaact cgatcaacgc gctcgacctg cgcaagtacc
      421 acacgcccgg cacgcagctg aaggagatct tccggccggc gccgatgctg tacctgaaca
      481 ccggcctgac ggaggggctg aacctcgagg gctactacca gttcacgtgg aacgcgttcc
      541 agttcgaccc ggtcggcacc ttcttttcgg ctgccgacgt ggtcggcaag gggcagcggc
      601 ccgcgttcat cccgtcgtcg ctgctgacgc cgttcgggct gtgcacgccg ccgaccccgt
      661 gcggtgacat cggcgacaag atccagccgg gcatcaacgt gattccgttc gagggcgaca
      721 agaagccgcc cgcaggacgc cagtgcgggc tcgcgctgcg cggcaagccg cgcggcatgg
      781 ataccgaatt cgcgctgtac tacatccgct accacgacaa gctgccgttc accacgctgt
      841 tcaccgatct gaacaatccg acctacgcca atgccaacgt gctcggggcc ggctacttca
      901 acgagttcgg caaggacaag gacctgttcg gcgtgtcgtt caacaccaag ctcggtccgg
      961 tggcagtcgg gggcgagctg tcataccgtc cgcgcgactc ggtcgccatc gatccctcgg
     1021 tgccggcgac cggcccatac agcatcttcg acccggcgcc gggcccgacc aagacggtcg
     1081 atccgaacgg ccgcatcacc gtgcgcggct tcgtcaacga gaagaagttc caggcccacc
     1141 tgacgggctt ctacttcatc gaggtcaact caccgatggg cgcgctgatg aaggggctcg
     1201 gcgcggccga gggctacgtg ctggccgaag cggcggtcac ccgctatccg gatctcgatc
     1261 cgtccaagat cccgtacctg atcttcccca gctacgagat tccgacgaag acctcggccg
     1321 gttacgtgat cgaggtcggg ctcacgtacc cgcacgcgtt cggcagcggc gtgaatctga
     1381 cgccgcagat cgacttcacg cacgacgtga ccggcatctc gcccaacacg ctgccgttcg
     1441 tcaagggccg caagtcgttc ttcttcggcg tcaactacga catcgacagc gtgtggcgcg
     1501 gccagatcgg cgtttcgcgc ttcttcggcg gtgacctcac caacgcgatc cgcgaccgca
     1561 gtttcttctc cgcgagcgtc tcgtactcgt tctgatgcag gacttcggca ggaccgtgcc
     1621 gcgttggaac gcggcacggt cggcaacgcg gtcgggccga tgatcgaact cgtcgtccgc
     1681 cggctcgagg cggtgatctt ccgtcaccgc gtcgcgacgc tggcgctgct cgccctgctc
     1741 accgtggtgc tcggggtgtc cgcgctgcag ctcaggatgg aggccggctt cgacaagcag
     1801 ttgccgaccg gccacgaata cacggacacg ttcttccagt atcgcgacca ggtcttcggc
     1861 gccaaccggc tgatggtcgc cgtcgcgcgc accaagggcg acgtgtggaa cgtcgccgcg
     1921 ctgcgcaagc tcaacgacgt caccgaggcg gtgctgtaca tgcccggcat cgaccgccgc
     1981 acggtgagtt cgctgtggac gccgaacacg cgcgtgctgc agatcaccga agaaggcttc
     2041 aagggcatgg acgtgatcgg cggcaacgtg accgccgaaa acctgaccga agcgcaggtg
     2101 gcgcgcatcc gcgacaacgc gatccagggc ggctatgtcg gcagcctggt ggccaacgac
     2161 gcgaccgcga cgatggtgat cgccgacctg ctcgactacg acccgaagac gcgcgcgaag
     2221 accgactaca tcgaactggg ccgccgcatc gaaacgcacc tgcgcgccaa gttcgaggac
     2281 gccgacacga agatccaggt gatcggcttc gccaagcaga tcagcgacat cgccgacggc
     2341 gcggcgagcg tgctgcgctt cttcgcgctc gccttcgtgc tgaccgcgct ggcggtctac
     2401 tggtacagcc gctcggtcgc gttcacggtc gcggcgatgc tgtgttcgct ggtgtccctg
     2461 gtctggcagt tcgggacgct gcatctgctc ggttacgggc tcgacccgct cgcggtgctg
     2521 gtgccgttcc tcgtgttcgc gatcggcgtg tcgcacggcg tgcagcaggt caactacatc
     2581 tcgcgctcga tctgcggcgg catgacgggc gcggacgcgg cgcgttccag cttccgcggc
     2641 ctgctggtgc cgggaacgat ggcgctggtc accgccttcg tcggctttgc caccctctat
     2701 ctggtgccga tcccgatgat ccgcgagctg tcgctgacgg catcgatcgg ggtcgcgtac
     2761 aagatcatca cgaacctggt gatgctgccg gtggtggcgt cgttcttcca cttcggcccc
     2821 gattacgtca agcgcgtcac tgcgctgcgg ctggagcgcg aagggtggat gaagcgtctc
     2881 gaccgcgtcg ccgagcgtca cgtcgcgttc gcggtcaccg gagcggcggt ggtcctattc
     2941 gggctcgcgg tgtggcaatc gagtgaccgc cacatcggct atctaaagcc cggggcgccc
     3001 gagttgcgcg aggattcgcg ctacaaccgc gatgccgtgg cgatcgtcga gaagttcggc
     3061 ctgggcctgg atgtcttcac gctggtgctc gaaatgccgc ccgacggctg ctacgagttc
     3121 gcgcgcatgt cgtacgtgga cgatttgtcg tgggtgatga ccaacgtgcc gggcgtgatc
     3181 ggcgtggcga gcctgtcggc gctggtgaag atcggcgcgt ccggcttcaa cgagggcaac
     3241 ccgaagtggc aggcgctgcc gcgcgacgcg cgctcaatgg cgagcttcat cggcctggcc
     3301 ggcgaaggct cgggcctgta cgacccgcgc tgctcggtgc tgccgatcaa cgtcagcctt
     3361 gccgaccaca aggcggtgac gctgcgggcg gtcgtcgatg cggccaagcg ttaccggcag
     3421 gacaacgtca acgacacggt caaggtgcgg ctggcgagcg gcaacgccgg cgtgcaggcg
     3481 gcaaccaacg aggtgctcga gcacacggaa ctgccgatga tgctctacgt gtacgtgacg
     3541 atcctgctgc tggtgctgat cacgtaccgc gactggcgcg cgatgatcgc ctgctgcgtg
     3601 ccgctgacgc tcgcgacgtt cctcggctac tggttcatga aggtgctcga catcgggctg
     3661 acggtggcca cgctgccggt gatggtgctg gcggtcggca tcggggtgga ctatgcgttc
     3721 tacatctaca accggctgca gacatatctc gccgatggca tgccgatcga accggcgttc
     3781 aagaacgcgt tgctggagac cggcaatgcg acgatcttca ccgcgatcac gctgtcgatc
     3841 ggcgtggcga cgtggggttt ctcggacctc aagttccagg cggacatggg gttgctgctg
     3901 gcgttcatgt tcatggtcaa catggtgatg gcgatcacgc tgctgcccgc gctggcggtg
     3961 gtgatcgatg cgctgatccc gcggcgcggc ccggtcaagc ggccgttgat ggcgcactga
     4021 catggcaccc cggctccttg cgctgtgcgc cgtacttctg attgcggtcg gcgcgctggt
     4081 gcacgcggcg gatccggcac ccgcggcggc gagcgcggca gcgccgggtg gcggtaacgc
     4141 gcggccgtcc aacctcgcgg tgtccaacct gctgcttgcc ggcacgcgcg ccggcgaacg
     4201 tctggtcgtg gtcggcgaat acggccatgt gctgctgtcg gacgacgacg gcgcgaactg
     4261 gcggcaggcg aagctcgttc ccacccgcac cacgctgacg gcggtgacgt tcatcgacgg
     4321 tcgcaccggc tgggccgtcg gccacggcgg catcgtgctc ggcacgcgcg acggcggcga
     4381 gaactggacc ctgctgtcgg ggcggatcga aggcaaggat gcgctgttct cggtctggtt
     4441 ccgcgacgag aagcacggcc tcgtggtcgg gccgtatgga tttgccgcgc gcagcgatga
     4501 cggcggcgcc acgtggaagt cgttcgagat cgcggagggc gaactcggcg agcggcacct
     4561 gaaccagatc gtcgcgcgcg
//
LOCUS       DBNHBY010000766         2400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_194449, whole genome shotgun sequence.
ACCESSION   DBNHBY010000766 DBNHBY010000000
VERSION     DBNHBY010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_194449"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="AC16HB_17995"
     CDS             <1..338
                     /locus_tag="AC16HB_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR5608737.1"
                     /translation="LKSGALRAIGVGAAARVAAAPEIPTIVEQGLPNYLVEGWFAAIG
                     PKGLPAADVKRIHAAIVTAFSSPEVKGAMDRQGNTINISTPEQAAQYFRSELAKYAIL
                     VKKAGVELQ"
     gene            complement(402..1337)
                     /locus_tag="AC16HB_18000"
     CDS             complement(402..1337)
                     /locus_tag="AC16HB_18000"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5608738.1"
                     /translation="MLLTTDARGVYPIAPTPFFDDGAIDTASIDRMTDFYLSIGATGI
                     TILGQLGEAPKLDHAESVAIAAQVIRRAPKLPVVVGVSAPGFAPMRALTRAVMDAGAA
                     GVMIAPPNTLRTDDQIVNYYRQAAEAIGADVPFVLQDYPLTFSVQMTPGVIRRIVTEV
                     QSCVMLKHEDWPGLEKISMLRGYERDDSMRHIAILCGNGGLFLDFEVERGADGANTGY
                     CFPDMLIDVVRLTAQGRRDEAHDLFDAHLPLLRYEQQPGVGLAVRKYVLMKRGILTSA
                     AQRKPVAAMSKAAQAEVDYLLARLSRRDPRANVAR"
     gene            1615..>2400
                     /locus_tag="AC16HB_18005"
     CDS             1615..>2400
                     /locus_tag="AC16HB_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608739.1"
                     /translation="MSLLAGVVWWVVLGVLLGWLASWLVGRGPRRPLPTPIERVVERT
                     VDNPEHLERIAALEQTAADAELLRERVRALESAAPAVVERVVERVVEKTVERPVDGTL
                     EKVVPDTEALAERDRQIEQWRERHDDLMQQLVAQRRRASELEGQVLRLRPPAVDLDMA
                     RAAGIALDGPDDFRVIAGIDDAIAARLRSEGIDSFHELARSAPARLRAILSDAGPDFR
                     SADPDGWPELALLAAHNHWRALKQLGETQSTGARTERERRDLES"
BASE COUNT          385 a          808 c          847 g          360 t
ORIGIN      
        1 aactgaagag cggtgcgctg cgcgcgatcg gtgtgggtgc cgccgcgcgc gtggccgcgg
       61 cgccggagat cccgacgatc gtcgagcagg ggctgcccaa ctacctcgtc gagggctggt
      121 tcgcggcgat cgggccgaag gggctgcccg cggccgacgt caagcgcatc cacgcggcga
      181 tcgtcactgc gttcagttcg cccgaggtca agggagccat ggacaggcaa ggcaacacga
      241 tcaacatcag cacgccggaa caggcggcac agtacttccg cagcgagctg gccaagtacg
      301 cgatattggt gaagaaggcg ggcgtcgagc tgcaatgaag cgcccgcgtt gctgatcgcg
      361 cactcggttg ctcttcgacg ctgccgcgcg cgcggcggcg gctaacgcgc gacgttcgcc
      421 cgcggatcgc gccgcgacag ccgcgcaagc agatagtcca cctccgcctg cgccgctttc
      481 gacatcgccg cgaccggctt gcgctgcgcg gcggaggtca ggatgccgcg cttcatcagc
      541 acgtacttgc gcaccgcgag cccgacgccg ggctgctgtt cgtagcgcag cagcggcagg
      601 tgcgcgtcga acagatcgtg cgcctcgtcg cgcctgcctt gcgcagtcag ccgcaccacg
      661 tcgatcagca tgtcgggaaa gcaatagccg gtgttggcgc cgtcggcgcc gcgctcgact
      721 tcgaagtcga ggaacagccc cccgttgccg cacagaatcg cgatgtggcg catcgagtcg
      781 tcgcgctcgt agccgcgcag catggaaatc ttctcgagcc ccggccagtc ctcgtgcttc
      841 agcatcacac acgactgcac ctcggtcacg atgcggcgga tcacgcccgg cgtcatctgc
      901 accgagaacg tcagcgggta gtcctgcagc acgaacggca cgtcggcgcc gatcgcctcg
      961 gccgcctgcc tgtagtagtt gacgatctgg tcgtccgtgc gcagcgtgtt gggcggcgcg
     1021 atcatcacgc cggccgcgcc ggcgtccatc accgcgcgcg tcagagcgcg catcggcgcg
     1081 aagcccggcg ccgagacgcc gaccacgacg ggcagcttcg gcgcacggcg gatcacctgc
     1141 gcggcgatcg cgaccgactc cgcgtggtcg agcttcggcg cttcgccgag ctggccgagg
     1201 atcgtgatgc cggtcgcgcc gatcgagagg tagaagtcgg tcatgcggtc gatcgacgcc
     1261 gtgtcgatcg cgccgtcgtc gaagaacggc gtcggcgcaa tcggatagac gccgcgcgcg
     1321 tcggtggtca gcagcatggg aggccctttc ggttcggcgc cggaatgtac gcgaccatgc
     1381 gctccggcac ttgattgcga ggcaatcatg aacccattcg atctcaccgg ggcaaggccg
     1441 ctatcgtgac ccaccgccac ggcggcatcg cgcccacgag cacgggcggc gcggcgtttc
     1501 cggggcccgc cgaacttgcg ttggcgcagt tccggcgcca gatcggattg cgcttgcagc
     1561 gcagtcacgg tatgaacgca aggcgtggat tcgcgcatgt gaccgaggtt gctcatgtcg
     1621 ttgctggccg gcgtcgtgtg gtgggtcgtt cttggcgtgc tgctcggatg gctggcgagc
     1681 tggctcgtcg gtcgcggtcc gcgtcggccg ctgccgacgc cgatcgagcg cgtggtcgag
     1741 cgcacggtcg acaaccccga gcacctcgag cgcatcgcag ctctcgagca gacggccgcc
     1801 gatgcggaac tcctgcgcga gcgcgtgcgc gcactcgaat cggcggcacc cgcggtggtc
     1861 gagagagtcg tcgagagggt cgtcgagaag accgtcgagc gtccggtcga cgggacctta
     1921 gagaaggtgg tccccgatac ggaggcgctc gccgagcgcg atcggcagat cgaacaatgg
     1981 cgcgaacgtc acgacgatct catgcagcag ctggtggcgc agcgccggcg cgcgagcgaa
     2041 ctggagggtc aggtgctgcg cctgcggcca ccggcggtcg atctcgacat ggcgcgcgcc
     2101 gccggaatcg cgctcgacgg gccggacgat ttccgcgtga tcgccggcat cgacgatgca
     2161 atcgcagcgc ggctcaggag cgagggcatc gactcgttcc acgaactcgc gcgctcggcg
     2221 ccggcacggc tgcgcgcgat cctttcagat gccggccccg atttccgcag cgccgatccc
     2281 gatgggtggc ccgagctggc cttgcttgcc gcgcacaacc actggcgcgc gctcaagcaa
     2341 ctgggcgaaa cgcaaagcac gggtgcacgc acggagcggg agcgacgcga cctcgaatcg
//
LOCUS       DBNHBY010000767         3655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1817912, whole genome shotgun sequence.
ACCESSION   DBNHBY010000767 DBNHBY010000000
VERSION     DBNHBY010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1817912"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC16HB_18010"
     CDS             complement(<1..587)
                     /locus_tag="AC16HB_18010"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR5608740.1"
                     /translation="MDLELIAQELQAAQDGARLIAPFTGRVPGFDNAAAYEVSRRLHA
                     LRLREGWRPVGRKIGFTNFNIWPEYGVYAPIWGYMYESTVAYASDQRANTRIGHLAQP
                     RIEPEVALHFKAVPPVTDDPSEILACVDWIAHGFEVVQSNFPDWKFAAADTVAQNGLH
                     GIYVIGTAKPVEELGPGLIARLGRFTVTLSCGGEVR"
     gene            628..1971
                     /locus_tag="AC16HB_18015"
     CDS             628..1971
                     /locus_tag="AC16HB_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR5608741.1"
                     /translation="MSLHQMLKKRAADGKPLRVGLIGAGKFGAMYLAQAPRTPGVLLA
                     GIADLSPAGARANLERVGWKPEQFGAASLDDALARGNTHLGEDWRKLVAHPAIDIIVE
                     CTGHPPAAVEHCLGAFAHGKHVVNVTVEADAFCGPLLARKAQEAGVVYSLAYGDQPAL
                     ICDLVDWARAAGFPVVAAGRGHKWLPHFSESTPDTVWGNYGLTPEQAQIGGLNPKMFN
                     SFLDGSKPSIESTAVCNATGLVAPPDGLTFPPASVDDIPFVCRPIAEGGVLHQKGQVE
                     VVSSLEKDGRAIPYDIRMGVFVVFEGETEYIRNCFKEYMVRTDPTGRYACLYKRWHLI
                     GLELGISVASVGLRREPTGAATCFNADVIATAKRDLKAGDMLDGEGGYTVWGKLFQAS
                     KSLAIGGLPLGLAHNIKLVRDVKKGQSLTWADVKIDEGLPAYRVRREMESQFGAR"
     gene            complement(1988..2236)
                     /locus_tag="AC16HB_18020"
     CDS             complement(1988..2236)
                     /locus_tag="AC16HB_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608742.1"
                     /translation="MHAKPTLSQPRGLTISIAAAFSAIVAVGSLTAVAWLFQREGMPF
                     EQLVAAERACAAHQYVSERESCIDDWLVAAKARRIARK"
     gene            complement(2344..2694)
                     /locus_tag="AC16HB_18025"
     CDS             complement(2344..2694)
                     /locus_tag="AC16HB_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608743.1"
                     /translation="MKIVALALAPALALLLLAAHFYRAGLWPLALAALLLNALLFVPR
                     AWAARTVQLALLAGAFEWLRTLASLVGERMAAGQPYLRLTAILLAVALLTAGAALAFR
                     AYPMRVRYGLGGRF"
     gene            complement(2699..>3655)
                     /locus_tag="AC16HB_18030"
     CDS             complement(2699..>3655)
                     /locus_tag="AC16HB_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5608744.1"
                     /translation="GTGDVVTARPDNDHASLFFGFPNSYGTLGYALRVRARTIPVEDH
                     VAVEQRRLARADDFFAQLAAAGASDADFVDGVVFGQSELVLSTARFVASAPYTSDYTY
                     EKIYYRSLREREHDYLTVRDYLWRWDTDWFWCSKNVGAQNPLLRRLYGRERLNSRTYQ
                     RIMRWNTRAGLMRGLERFSGWHRESIIQDVDIPLAHAPEFLDFYLREIGLLPLWICPI
                     GAAVDCDRFALFPRAQATYVNFGFWDVKRTREPHTPGHFNRLIERKVSALGGIKSLYS
                     DSYFTRAEFDAAYGGDAYRALKSRYDAAGRLGDLYAKCVLKQ"
BASE COUNT          613 a         1196 c         1287 g          559 t
ORIGIN      
        1 cgaacttcgc cgccgcacga cagcgtgacg gtaaagcgcc ccagccgcgc aatgaggccg
       61 gggccgagtt cttcgacggg cttggccgtg ccgatcacat agatgccgtg cagcccgttc
      121 tgcgcgaccg tgtcggcggc ggcgaacttc cagtcgggaa agttcgactg cacgacttcg
      181 aagccgtgcg cgatccagtc tacgcaggcg aggatctcgg acggatcgtc ggtcacaggc
      241 gggacggcct tgaagtgcaa tgcgacctcc ggctcgatgc gcggctgcgc cagatgcccg
      301 atgcgcgtgt tcgcccgctg atcggacgcg taggcgacgg tcgactcgta catgtagccc
      361 cagatcgggg cgtaaacgcc gtactcgggc cagatgttga agttggtgaa gccgatcttg
      421 cgcccgacgg gtctccatcc ttcgcgcagc cgcagcgcgt gcagccggcg cgagacttcg
      481 tacgcggcgg cgttgtcgaa gcccggcacg cggcccgtga agggcgcgat caagcgcgcg
      541 ccgtcctggg ccgcctgcaa ctcttgcgcg atcagttcga ggtccatggc ttgtcgcaga
      601 atgtggaaat cgaaccggga gtcgccgatg agtctgcacc agatgctgaa gaaacgcgcc
      661 gccgatggca agccgctgcg cgtgggattg atcggcgccg gcaagttcgg cgcgatgtac
      721 ctcgcgcagg cgccgcgcac gccgggcgtg cttctggcag ggatcgccga cctgtcgcct
      781 gcgggtgcgc gcgccaatct cgaacgcgtg ggctggaaac cggagcagtt cggcgccgcg
      841 tcgctcgatg acgcgctcgc caggggcaac acgcatctgg gcgaggactg gaggaagctg
      901 gtcgcccatc cggcgatcga catcatcgtc gagtgcaccg gccatccgcc ggccgcggtc
      961 gagcattgcc tgggcgcgtt cgcgcacggc aagcacgtgg tcaacgtaac ggtcgaggcc
     1021 gacgcgttct gcggcccgct gctcgcgcgc aaggcgcagg aagccggcgt cgtctacagc
     1081 ctcgcgtacg gcgaccagcc agcgctgatc tgcgatctcg tcgactgggc gcgcgcggcg
     1141 ggtttcccgg tcgtcgcggc ggggcgcggc cacaagtggc tgccgcattt ctccgagtcc
     1201 acgccggata cggtgtgggg caactacggg ctgacgcccg agcaggcgca gatcggcggg
     1261 ctgaatccca agatgttcaa ttcgttcctc gacgggtcca aaccgtcgat cgagtcgact
     1321 gccgtatgca acgcgaccgg gctggtcgcc ccgcccgacg ggctcacttt cccgccggcg
     1381 tcggtcgacg acatcccgtt cgtatgccgc ccgatcgccg agggtggcgt gctgcaccag
     1441 aaggggcagg tcgaagtggt ctcgtcgctg gagaaggacg gccgcgcgat cccgtacgac
     1501 attcgcatgg gcgtattcgt cgtgttcgag ggcgagaccg agtacatccg caactgcttc
     1561 aaggaataca tggtgcgcac cgatccgacc ggccgctacg cgtgcctata caagcgctgg
     1621 cacctgatcg ggctcgaact cggcatctcg gttgcgtcgg tcgggctgcg gcgcgaaccg
     1681 accggcgcgg cgacctgttt caacgcggac gtgatcgcga ccgccaagcg cgatctgaag
     1741 gccggcgata tgctcgacgg cgaaggcggc tacaccgtgt gggggaagct tttccaggcg
     1801 agcaagtcgc ttgcaatcgg cggcctgccg ctcgggctcg cgcacaacat caagctcgtg
     1861 cgcgacgtga agaagggcca gtcgctgacc tgggccgacg tgaaaatcga cgaaggcctg
     1921 cccgcctatc gagtccggcg ggagatggag tcgcagttcg gcgcacgctg agcgcgcgcc
     1981 gcgtcgatca cttgcgggcg atccggcgcg ccttggcggc gacgagccag tcgtcgatac
     2041 agctttcgcg ctccgacacg tactgatgcg cggcacaggc gcgctcggcg gcgaccagtt
     2101 gttcgaaggg catgccctcg cgctggaaca gccacgcaac cgcggtcagt gaaccgactg
     2161 cgacgatggc tgagaacgcg gcggcgatgc tgatcgtcag gccgcgcggc tgactcaagg
     2221 tcggtttggc gtgcaaggcg gtctccagaa gatgcgaccc tccagggtgt cgccgcagcc
     2281 ggaagccggg gcgcctgggg tgaccttgag ttgccagttg ccgaggacgc gttcaatccg
     2341 gattcagaac cgcccgccga ggccgtaacg cacgcgcatc gggtaggcgc ggaacgccag
     2401 cgcggcgccg gcggtcagca gcgcaaccgc gagcaggatc gccgtcaggc gcaggtaggg
     2461 ctgcccggcc gccatccgtt cgccgaccaa tgacgccagc gtacgcagcc actcgaacgc
     2521 gccggcgagc agtgccagtt gtaccgtccg cgccgcccag gcgcgcggca cgaagagcag
     2581 cgcgttgagc agcagcgccg ccagcgcgag cggccacagc ccggcgcgat agaaatgcgc
     2641 ggcgagcagc agcagtgcca gggcgggagc aagcgcgagc gcaacgatct tcatcgtttc
     2701 attgcttcag cacgcatttg gcgtagaggt cgccgagccg gccggccgca tcgtagcggg
     2761 acttcagcgc ccggtacgcg tcgcccccat acgcagcgtc gaactccgcg cgcgtgaagt
     2821 agctgtccga gtagagcgac ttgattccgc ccagcgcgct caccttgcgc tcgatcagcc
     2881 ggttgaagtg cccgggcgta tgcggctcgc gcgtgcgctt gacgtcccag aagccgaagt
     2941 tcacgtacgt cgcctgcgcg cgcggaaaca gcgcgaagcg atcgcagtcg accgcggcac
     3001 cgatggggca gatccacagt ggcaggaggc cgatctcgcg caggtagaag tcgaggaact
     3061 ccggcgcgtg tgccagcgga atgtccacgt cctggatgat cgactcgcgg tgccagccgg
     3121 agaatcgctc gaggccccgc atcaggcccg cgcgggtgtt ccagcgcatg atgcgctggt
     3181 atgtgcgcga attcagccgc tcgcgcccgt acagccggcg cagcagcggg ttctgcgcgc
     3241 cgacgttctt cgagcaccag aaccagtcgg tgtcccagcg ccagaggtaa tcgcggacgg
     3301 tcaggtagtc gtgctcgcgc tcgcgcagcg atcgatagta gatcttctcg taggtgtagt
     3361 cggaggtgta cggcgcgctg gcgacgaagc gcgcggtgct gagcaccagc tcgctctggc
     3421 cgaacacgac cccgtcgacg aaatcggcgt cgctcgcgcc ggcggcggcg agctgagcga
     3481 agaagtcgtc cgcgcgtgcc aggcgccttt gctcgaccgc gacgtggtcc tcgaccggga
     3541 tggtgcgtgc gcgcacgcgc agcgcatagc cgagcgtgcc gtacgagttg ggaaagccga
     3601 agaacaggct cgcgtggtcg ttgtccggcc gcgcggtcac cacgtcgccc gtgcc
//
LOCUS       DBNHBY010000768         1849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1479922, whole genome shotgun sequence.
ACCESSION   DBNHBY010000768 DBNHBY010000000
VERSION     DBNHBY010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1479922"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16HB_18035"
     CDS             <1..417
                     /locus_tag="AC16HB_18035"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR5608745.1"
                     /translation="GELDADRARQVAHGLVQPSWRSSVWRVDRIGAFERARVLWAGSE
                     DDPTLAALAAAVRAMLDDLQVRYDRKPFAPHVTLLRNLPRAAAGTAQPIEPPIDWVTQ
                     PPVLLYSTHEADGTRYVVCESDNAAFSASSARDTSR"
     gene            complement(383..1792)
                     /locus_tag="AC16HB_18040"
     CDS             complement(383..1792)
                     /locus_tag="AC16HB_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR5608746.1"
                     /translation="MQVQSMHFKSRAAMKLADARLQKNLANVRDKFTRKRALAIAELD
                     DFEAIRAAAVERRDRGLENLDAWIEQFERTATARGATVLFAQSHAEAADLIIDIARRH
                     GVRKVTKSKSMVSEEVGLNRALAAAGVQPVETDLGEYILQINDNEPPSHIIAPVMHKD
                     KEEISDLFAKVHATPRKTEITQLTGEARAVLRQHFLSADMGVTGANFLIAETGSVALV
                     TNEGNEGMCTILPRVHVVLAGVEKVLPTLEDLATVMRLLPRSATGQAISNYFSILTGT
                     RAAGESDGPEHLYYVLVDAGRTGLLGGEFQDMLRCIRCGACMNHCPVYQKIGGHAYGW
                     VYPGPMGSVLTPSYVGLENALDLPQAATLCGQCEVVCPVKIPLPELLRKLRQKQFERR
                     LRPWPERAGFAVWGYVAQRPALYRLVVRVAARVLQWMGGQQRRIRALPLAGGWTSQRD
                     FPAPGGRTFIDLYRERSMR"
BASE COUNT          294 a          640 c          592 g          323 t
ORIGIN      
        1 ggcgaactcg atgcggatcg cgcgcggcag gtcgcgcatg gccttgtgca gccgtcctgg
       61 cgctcgagcg tctggcgcgt cgaccgcatc ggcgcgtttg agcgtgcgcg tgtgctttgg
      121 gccggcagcg aagacgaccc gaccctcgcg gcgcttgccg ccgcggtccg ggcgatgctg
      181 gacgatctgc aagtgcggta tgaccgcaag ccgttcgccc cgcacgtcac gctgctcagg
      241 aatctgccac gcgccgccgc cggtaccgcg cagccgatcg agccgccgat tgattgggta
      301 acgcagccgc ccgtgctgct gtattcgacc cacgaggcgg acgggacacg ctacgttgtc
      361 tgcgagtcgg acaacgccgc gttcagcgca tcgagcgctc gcgatacaag tcgatgaacg
      421 tccggccgcc aggagccgga aaatcgcgtt gcgaggtcca gccgccggcc aacggcagcg
      481 cgcggatgcg gcgctgctga ccacccatcc actgcagtac acgcgccgct actcgcacga
      541 caagtcggta caacgccggc cgctgggcca cataacccca gaccgcgaat cccgcgcgtt
      601 ccggccacgg gcgcagccgg cgctcgaact gcttctgcct cagcttgcgc agcagttcgg
      661 gcagcgggat cttcacgggg cacacgacct cgcactgtcc gcacagcgtc gccgcctgcg
      721 gcaggtcgag cgcgttctcc aagccgacat agctcggcgt gagcaccgaa cccatcgggc
      781 ccggataaac ccagccgtac gcgtgcccgc cgatcttttg gtacacggga cagtggttca
      841 tgcaggcgcc gcatcggatg cagcgaagca tgtcctggaa ttcgccacca agcaggcccg
      901 tgcggccggc atcgaccagc acatagtaga gatgctccgg cccgtcgctt tcacctgccg
      961 cacgcgtgcc ggtgaggatc gagaagtagt tcgagatggc ctgcccggtc gccgaacgcg
     1021 gcagcaggcg catcacggtc gctaggtctt ccagcgtcgg cagcaccttc tctacgcctg
     1081 ccagcacgac gtgcacgcgc ggcaggatcg tgcacatgcc ctcgttgcct tcgttcgtca
     1141 ccagcgcgac cgagccggtt tcggcgatca ggaagttcgc gcccgttacg cccatgtcgg
     1201 ccgacaggaa gtgctggcgc aacacggcac gcgcctcgcc cgtcagttgc gtgatttccg
     1261 ttttgcgtgg cgtggcgtga accttcgcga agagatcaga gatctcttcc ttgtccttgt
     1321 gcatcaccgg cgcaatgatg tgagacggtg gctcgttgtc gttgatctgc aggatgtact
     1381 cgcccaagtc ggtttcgacc ggctgcaccc ccgcggcggc cagcgcgcga ttcaggccga
     1441 cctcctccga aaccatcgac ttcgacttgg tgacctttcg gacgccgtgc cggcgtgcga
     1501 tgtcgatgat caggtcggcc gcctccgcat gtgactgtgc aaacagcact gtggcgccac
     1561 gcgcggttgc ggtgcgctcg aactgctcga tccaggcgtc gaggttctcg aggccgcggt
     1621 cgcggcgttc gaccgcggcc gcgcggatcg cctcgaaatc gtccagttcc gcgatggcca
     1681 acgcccgctt gcgcgtgaac ttgtcgcgca cgttcgcaag gttcttctga agtcgcgcgt
     1741 cggcgagctt catcgccgcc cgcgacttga agtgcatcga ctgcacctgc atcaggcgtc
     1801 ccccgccagg atttgcgcga tgtgcagcac gcgcgtcttc tcgtcgccg
//
LOCUS       DBNHBY010000769         5285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_939126, whole genome shotgun sequence.
ACCESSION   DBNHBY010000769 DBNHBY010000000
VERSION     DBNHBY010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_939126"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..795
                     /locus_tag="AC16HB_18045"
     CDS             205..795
                     /locus_tag="AC16HB_18045"
                     /inference="COORDINATES: protein motif:HMM:NF017736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HIR5608747.1"
                     /translation="MSTSGSRRRLLLACGCCVLAPLVTEAKTEGDAAPEDWRASLLRD
                     RRELWLVRDDDEVRATYWTAATGFDRNQYRQLCWILRDKRTGSSFPIDRNLLDVLAGI
                     QAWLAHEGHAGPIRVNSGYRTMATNRRTEGAARNSQHVIGKAADIVVPGVSPVKLAGM
                     ASLFGRGGAGFYVGREFVHVDTGDERIWIDQKRRTS"
     gene            complement(788..2362)
                     /locus_tag="AC16HB_18050"
     CDS             complement(788..2362)
                     /locus_tag="AC16HB_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608748.1"
                     /translation="MSSPPRSRASSLDDTQVQRSSPLISSGAGDAVGVAFPRAGGGDR
                     SSLMAGKRLFAEAIQIASPALADAIVDEPDWRHRYPAHIRAQVELMVAEPGETVAIAR
                     RALDTVYRSFEFCRDGAAMPIDLALRAPCTDPLYTVTIAGHGKETPRLEVPYRGERLA
                     GSALVAQVHDWVERGIAEPSFAAAIETVVAHPDWLDLSDWNIAVLGAGAELNPLATLA
                     AWRATLIAVDVPDPALWQRLIDIARAGNGRVRIPVREGSSADDSRLAELAGADLLTAT
                     PQIRNWLVSMPEPLALGCFAYLDGEAHVRLAVAMDALINDLLALRRNVVPAFLLTPTD
                     CYAVPAYTAERAVTRWYTRGAHRMWQEPLRWLTRDRLFASNVAQVLEGPDGFRAGVVD
                     ALVVEQGPNYALAKRLQQWRALVAHADGLRVSVNVAPVTRTQSVIRHRAMRAAYAGAH
                     MFDVEVFEPATASALMAALLVHDLRTSSGASAPDAVLAHPLLLFTEGALHSGLWTLPF
                     SARSALPIAALRGLLS"
     gene            complement(2400..3302)
                     /locus_tag="AC16HB_18055"
     CDS             complement(2400..3302)
                     /locus_tag="AC16HB_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR5608749.1"
                     /translation="MAQLSSPFDLADAAAYRAWRGRKLAGYPASLDELVVEVADPLHL
                     TVAERGALLECCARANMAVYACARTPAEAADIPRTIGSQLGLRTLDVNYLADDDGITP
                     LAVAADGTRSAYIPYTNRAIRWHTDGYYNPPERRIRGMILHCVQRAEVGGENRLLDHE
                     LAYIALRDADPAHIRALMLPDAMTIPARSEEGEVARDDQTGPVFSLVDDGRLHMRYTA
                     RTRSIVWKDDPAVAAAIAALGRILEHDPHVLRAQMQPGMGLVCNNVLHDRSAFTDSDA
                     RRRLVLRARYYEAIVPTPATALAA"
     gene            complement(3305..3985)
                     /locus_tag="AC16HB_18060"
     CDS             complement(3305..3985)
                     /locus_tag="AC16HB_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608750.1"
                     /translation="MSSASILRRTKSRFFAKDKGRDPETLASIAAFTAWRLGLNTIKR
                     MRSAQFEIEANAQYFAFLREYLVFLIQVTDRFAFSRLSLPERRVFTVSMVKRLAEQQA
                     ENQSRLLGDAHDEVRREFIDLFNSRADQYAECGFDDAQGPGLDFMRTCSVALQAVVAP
                     QDRAWITDQVMSVEAPDAVETLRKVVDNAVRTAGSIIAPPDEPHEPGPLPEMPGMDAP
                     VDDPIPPE"
     gene            complement(4020..4349)
                     /locus_tag="AC16HB_18065"
     CDS             complement(4020..4349)
                     /locus_tag="AC16HB_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR5608751.1"
                     /translation="MAYEVNGRTLETDEEGYLLEPNYDDEVCPVIAQAEGITLTDEHW
                     NVIRYLRDQYREHGQTPNYRNMLKEVAELMPQHDSKTLYDLFPGGPAKQGVRVAGLPK
                     PFGKGGY"
     gene            complement(4357..4836)
                     /locus_tag="AC16HB_18070"
     CDS             complement(4357..4836)
                     /locus_tag="AC16HB_18070"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5608752.1"
                     /translation="MNDTSIVRLDMTGQSCPLPLLGAKKILDDLPDGQRMVLVSDCPG
                     TGDDLRVWAEQTGHQVLHIERLDGKRVAYTLQRGRAAARTTGNVMLDLRGVTCPGPIV
                     EAHRLLKGMHSGEVLVLISNCPGAPADVAAWADGANVRLIDQYESAPGEYEFYLRKE"
     gene            complement(4858..>5285)
                     /locus_tag="AC16HB_18075"
     CDS             complement(4858..>5285)
                     /locus_tag="AC16HB_18075"
                     /inference="COORDINATES: protein motif:HMM:NF021100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HIR5608753.1"
                     /translation="IGGRTRLVLRLPAERAQECAGLQGARLELPAPLEVGRFTPRDLL
                     PYPVLHARLVVTGAADEGDFLADVERAAAELEIDADAIVGRRGEIAFEERKDTGFSLM
                     LHGLSPEDSLRLQQHGIGLHRMLGCGLFVPHKSIAAVGA"
BASE COUNT          794 a         1839 c         1860 g          792 t
ORIGIN      
        1 tggcggggga gacgcctccg cacgaaggcc gtcccggcgt gctgcgcgca cggaccgagg
       61 agacgctgcg cgcggtgcag gcgctcgatc gcggcggcgc cacctgaccg caacggccag
      121 gcctgcaacg gcggcatccc cgctggccga acggcccgga gcgctcgtac aattcgtcgc
      181 tatggcgtgg gcagggtgct tcccatgagc acaagcggat cgagacggcg gttgctgctg
      241 gcgtgcggtt gctgcgtgct ggcgccgctc gtcaccgagg caaagacaga aggcgatgcg
      301 gcgccggagg actggcgcgc gtcgctgttg cgcgacaggc gcgaactctg gctggtgcgc
      361 gacgacgacg aggtgcgcgc gacctactgg accgcggcga ccggattcga tcgcaaccag
      421 taccggcagt tgtgctggat cctgcgcgac aagcgcaccg gcagcagctt cccgattgac
      481 cgcaatctgc tcgacgtgct ggccggcatc caggcgtggc tcgcgcacga ggggcatgcc
      541 ggaccgatcc gcgtcaattc cggctatcgc acgatggcca cgaaccgccg caccgagggc
      601 gcggcgcgca actcccagca cgtcatcggc aaggcggccg acatcgtcgt gcccggcgtg
      661 tcgccggtga agctcgccgg catggcgagc ctgttcggcc gcggcggcgc gggcttctac
      721 gtcggccgtg agttcgtgca cgtcgacacc ggcgacgagc ggatctggat cgaccagaag
      781 cgccgcacta gctgagaagg ccgcggagcg cggcgatcgg cagtgccgac cgcgccgaga
      841 acggcagggt ccacaggccg ctgtgcagcg cgccttccgt gaacagcagc agcgggtgcg
      901 cgagcactgc gtcgggcgcc gaggcgccac tggaggtgcg caggtcgtgc accagcagcg
      961 ccgccatcag cgcgctggcc gtcgccggct cgaaaacctc cacatcgaac atgtgcgcgc
     1021 ccgcataggc ggcgcgcatc gcgcggtgcc ggatcaccga ctgcgtgcgc gtcaccgggg
     1081 ccacgttgac ggacacgcgc agcccgtcgg cgtgcgcgac cagcgcgcgc cactgctgca
     1141 ggcgcttggc gagcgcatag ttcggtccct gctcgacgac gagcgcgtcg acgacgccgg
     1201 cgcggaagcc atccggtccc tccagaacct gtgcaacgtt ggacgcgaac aggcgatcgc
     1261 gggtgagcca gcgcagcggt tcctgccaca tccgatgcgc gccgcgcgtg taccagcgcg
     1321 tgaccgcgcg ctcggcggtg tacgcaggca ccgcgtagca gtcggtcggc gtcagcagga
     1381 acgccggcac gacgttgcgc cgcagcgcga gcagatcgtt gatcagtgca tccatcgcga
     1441 cggcgagccg gacgtgcgct tcgccgtcca gataggcaaa gcagccaagc gcgagcggct
     1501 ccggcatcga aaccagccag ttgcggatct gcggtgtcgc cgtgagcaga tcggcgccgg
     1561 cgagttcggc cagtcgcgaa tcgtcggccg acgagccctc gcgcacgggg atgcggacgc
     1621 gcccgttgcc agcgcgcgcg atgtcgatca gccgttgcca cagcgccgga tctggcacgt
     1681 cgacggcgat cagcgtcgcg cgccatgccg ccagcgtcgc aagcgggttc aactcggcgc
     1741 cggcgccgag cacggcgatg ttccagtccg aaagatcgag ccaatccggg tgcgcgacca
     1801 ccgtttcgat cgcggcagcg aacgagggct cggcgatgcc gcgctcgacc caatcgtgca
     1861 cctgcgcgac cagcgccgat cccgccagtc gctcgccgcg atagggcacc tcgagccgcg
     1921 gcgtctcctt gccgtggcct gcgatcgtga ccgtgtatag cgggtccgtg cacggtgcgc
     1981 gcagcgcgag gtcgatcggc atcgccgcgc cgtcgcggca gaactcgaag ctgcggtaga
     2041 cggtgtcgag ggcgcgccgc gcgatcgcga cggtttcgcc cggctcggcg accatcagct
     2101 cgacctgcgc gcggatgtgc gcgggatagc gatggcgcca atcgggctcg tcgacgatcg
     2161 cgtcggccag cgcgggcgag gcgatctgga tcgcttcggc gaacaggcgc ttgccggcca
     2221 tcagcgagct gcgatcgcca ccgccggcgc gcggaaacgc gacccccacc gcatcgcccg
     2281 cgccgctgga gatcaacggc gacgagcgct gcacctgcgt gtcgtcgagc gaggaggcgc
     2341 gtgagcgcgg gggggaactc atgcggcggt gagtctaccg acccgcgccg aacgtgacat
     2401 caggctgcca gcgcggtcgc cggcgtggga acgatggctt cgtaatagcg cgcccgcagc
     2461 accaggcggc gccgcgcgtc cgagtcggtg aacgccgaac ggtcgtgcag cacattgttg
     2521 cagaccagtc ccatgccggg ctgcatctgc gcgcgcagca cgtgcgggtc gtgctcgagg
     2581 atccgcccca gcgctgcgat ggccgcggcc acggccgggt cgtctttcca cacgatgctg
     2641 cgcgtgcgcg cggtgtagcg catgtgcagc cggccgtcgt cgacgagcga gaacaccggg
     2701 cccgtctggt cgtcgcgcgc gacttcgcct tcctccgagc gcgccgggat cgtcatcgcg
     2761 tcaggcagca tcagcgcgcg gatgtgcgcg ggatccgcgt cgcgcagcgc gatgtaggcg
     2821 agttcgtgat cgagcaggcg gttctccccg ccaacctccg cgcgctgcac gcagtgcagg
     2881 atcatcccgc ggatgcggcg ctcggggggg ttgtagtaac cgtcggtgtg ccagcggatc
     2941 gcgcggttgg tgtacgggat gtacgcgctg cgcgtgccgt ccgcggccac cgccagcggc
     3001 gtgatgccgt cgtcgtcggc gagatagttg acgtcgaggg tgcgcaggcc gagctgcgat
     3061 ccgatcgtgc gcgggatgtc ggcggcctcg gccggcgtcc gcgcgcaggc gtagacggcc
     3121 atgttggcgc gcgcgcagca ttcgagcagg gcaccgcgct ccgcgaccgt caggtgcagc
     3181 ggatcggcaa cttcgaccac gagttcgtcg agcgacgccg gatacccggc cagcttgcgg
     3241 ccgcgccatg cccggtacgc ggcggcgtcg gccagatcga acggtgagga cagctgcgcc
     3301 atggctactc aggcggaatc gggtcgtcga cgggggcatc cattccgggc atctcgggca
     3361 gcgggccggg ctcgtgtggc tcgtccggcg gcgcgatgat cgagccggcg gtcctgaccg
     3421 cgttgtcgac caccttgcgc agcgtctcga ccgcatcggg cgcctcgacc gacatcacct
     3481 ggtcggtgat ccatgcgcgg tcctgcggcg cgaccacggc ctgcagcgcg acgctgcagg
     3541 tgcgcatgaa gtcgagaccg ggcccttgcg cgtcgtcgaa cccgcactcg gcgtactgat
     3601 cggcgcgcga attgaagagg tcgatgaact cgcgccgcac ttcgtcgtgc gcatcgccga
     3661 gcagccgcga ctggttctcc gcctgctgct cggccaggcg cttgaccatc gacacggtga
     3721 acacgcggcg ctccgggagc gacaggcgcg agaacgcgaa acgatccgtc acctggatca
     3781 ggaacaccag atactcgcgc aggaacgcaa agtactgcgc gttggcctcg atctcgaatt
     3841 gcgcgctgcg catgcgcttg atcgtgttca gcccgagccg ccacgccgtg aacgcggcaa
     3901 tgctggcgag cgtctccgga tcgcgcccct tgtccttggc gaagaagcgg ctcttggtcc
     3961 gccgcagtat cgaggcgctg ctcacaaaga ccttccgtgg gccctgacgt atcgcagcgt
     4021 cagtagccgc ctttgccgaa gggcttcggc agcccggcga cgcggacgcc ctgcttggcc
     4081 gggccacccg ggaacaggtc gtacagcgtc ttgctgtcgt gctgcggcat cagctcggcc
     4141 acttccttca gcatgttgcg gtagttcggt gtctgaccgt gctcgcggta ctggtcgcgc
     4201 agatatcgga tcacgttcca gtgttcgtcc gtcagtgtga tcccttcggc ctgcgcgatc
     4261 accggacaga cctcgtcgtc gtagttcggc tccagcagat agccctcttc gtcggtctcg
     4321 agggtccgcc cgttcacctc gtatgccatc gtgctttcac tccttgcgca gatagaactc
     4381 gtactcgccg ggcgctgatt cgtactgatc gatcaggcgc acgttggcgc catcggccca
     4441 ggccgcgaca tcggccggcg cgccggggca gttggaaatc agcaccagca cctcgccgct
     4501 gtgcatgccc ttcagcagcc ggtgcgcctc gacgatcggc cccgggcagg tgactccgcg
     4561 gaggtcgagc atcacgttgc cggtcgtccg ggcggcggcg cggccgcgct gcagcgtgta
     4621 ggcgacgcgc ttgccgtcca accgctcgat gtgcagcacc tggtggccgg tctgctccgc
     4681 ccatacgcgc aggtcgtcgc ccgtgcccgg gcagtcagac accagcacca tccgctggcc
     4741 atcgggcagg tcgtcgagga tcttcttcgc gcccagcagt ggcagcgggc acgactgacc
     4801 cgtcatgtcg agccgcacga tcgatgtgtc gttcattggc ctgctctccc tgatcactca
     4861 agcgccgacc gccgcgatcg acttgtgcgg cacgaacagc ccgcacccca gcatccggtg
     4921 cagcccgatg ccatgctgtt gcagccgcag cgagtcctcg ggcgacaggc catgcagcat
     4981 caggctgaaa ccggtgtctt tccgctcctc gaacgcgatc tcgccacggc ggccgacgat
     5041 tgcatcggcg tcgatttcga gttccgcggc cgcgcgctcg acgtccgcga ggaagtcgcc
     5101 ttcgtccgcg gcgccggtca ccaccagccg cgcgtgcaac accgggtacg gcagcagatc
     5161 gcgcggcgtg aatcgcccca cctcgagcgg cgccggcagt tcgagccgcg cgccctgcag
     5221 gccggcgcac tcctgcgcgc gctcggcggg cagccgcagc accaggcgcg tgcggccgcc
     5281 gatca
//
LOCUS       DBNHBY010000770         6710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1684771, whole genome shotgun sequence.
ACCESSION   DBNHBY010000770 DBNHBY010000000
VERSION     DBNHBY010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1684771"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC16HB_18080"
     CDS             complement(<1..349)
                     /locus_tag="AC16HB_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HIR5608754.1"
                     /translation="MNAMLRNALAMAAVAIATQATAQVTFYEREDFQGRTFTTERQIG
                     NFERFGFNDRASSVVVLRDRWEVCEDVRFSGRCVVLRPGRYPSLAAMGLNDRISSVRA
                     VSGSARIDSNRYAP"
     gene            673..1278
                     /locus_tag="AC16HB_18085"
     CDS             673..1278
                     /locus_tag="AC16HB_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608755.1"
                     /translation="MSKLMIALLATAGLGITGHAAAQTYSSKSTSTPMSKDTYSMATK
                     GADAQYKIDKDACSSLSANAKDICLAEAKGKHNVAKADAEAAYKHTPKARENARVARA
                     QANYDVAIEKCDDLAGNVKNVCVKEAKAELVKGKANAKVDRVAADTSQDAAAKQADAR
                     KDANADMRDAEYKVAIEKCDALAGAAKDGCVNSAKAKFGKS"
     gene            1409..2305
                     /locus_tag="AC16HB_18090"
     CDS             1409..2305
                     /locus_tag="AC16HB_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5608756.1"
                     /translation="MRPFGRQAAYVATHPGAFALRVLRAFGANQGLLLAGAIAYYTLL
                     SIVPLLILVLIALSQIVAQGELLATLGRYLEWLMPGQSTAVIAELARFLDHRGVIGWV
                     LLATMIFFSSLAFRVLESAMSVIFLHRVAARRRRFVVSAVIPYCYIVVLGLGLLLMTL
                     VSGSLQAIGAESIDFLGRNWSLRGFSGALLYLLGVIGEILVLTSIYLVMPVGRPSWRH
                     ALIGGITATALWEITRHVLVWYFGTLSQVTVVYGSLTTSIVVLLSLEIAATLLLLGAQ
                     VIAEYERIGTDGQDAAPPPLWT"
     gene            complement(2346..2837)
                     /locus_tag="AC16HB_18095"
     CDS             complement(2346..2837)
                     /locus_tag="AC16HB_18095"
                     /inference="COORDINATES: protein motif:HMM:NF045536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070088 - polyhydroxyalkanoate granule
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate granule-associated phasin"
                     /protein_id="HIR5608757.1"
                     /translation="MKRNHFGNPFLAPFMLWNSLFMKTAEMMLASAQVIGHRTARMAR
                     AGAAPTARDRREFALMGQEKIEAGAQSAQAMAAQLMTARRPAGARALESAQRSTAAFM
                     SLAASRTPAQVIARQAALARALGQSATSVAGASKSAARLAQRGLKPIHAKATANARRL
                     ARR"
     gene            complement(2892..3635)
                     /locus_tag="AC16HB_18100"
     CDS             complement(2892..3635)
                     /locus_tag="AC16HB_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5608758.1"
                     /translation="MSLPHSPNQNHLLAALPTAEFERLAPHLELVPMLLGESLYEPGV
                     QLQYVYFPTTAIVSLLYVLESGASAEIAGVGNEGILGISLFMGGDTTPSSAVVQTAGH
                     GYRLPGRLLKEEFNRAGLMQRLLLRYTQALLTQMCQTTACNRHHSIEQQLCRWLLLTL
                     DRLPSNELVMTQELVASALGVRRESITEAAGKLQRAGLIRCRRGQIAVLERSGLEAGA
                     CECYAVVKKEIGRLLSDVRYRQGITASAI"
     gene            complement(3638..4480)
                     /locus_tag="AC16HB_18105"
     CDS             complement(3638..4480)
                     /locus_tag="AC16HB_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608759.1"
                     /translation="MVSANEILNASILIVDDQEANVQLLEQLLSDAGYTCVASTMNPT
                     EVFTLHRRHRYDLILLDLQMPGMDGFQVMEGLKTSDSDSYLPVIVLTAQPGHKLRALQ
                     AGAKDFISKPFDLIEVKTRIRNMLEVRLLYRKLEEFNQVLERTVQERTAELRESEARY
                     RSLTELASDWYWEQDERGNFTKVSGPVLEMLGIRVPAFAAEADDDQIKGWNEAERAEL
                     QARIAARQPFLDFVFSRVNADGSEQRFQVSGEPMFNRSCRFIGYRGIGVELIAGNSPP
                     GLDR"
     gene            complement(4480..6183)
                     /locus_tag="AC16HB_18110"
     CDS             complement(4480..6183)
                     /locus_tag="AC16HB_18110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR5608760.1"
                     /translation="MFESDSVILQAAGEGDRSAEVRRQETLLKTGALQRAILTSASFS
                     IIATDEKGIVQVFNIGAERMLGYSAPEVVNRISPSDMHDPQEVVARARALSLELATPI
                     APGFEALSFKASRGIEDSYELTYIRKDGSRFPATVSITALRNDDGEIIGYLLIGADNS
                     ARKQIELELHDAMAAAEKANLAKSDFLSSMSHELRTPLSAILGFAQLMESGSPSPTVS
                     QKRSIDQILKAGWYLLDLINEVLDLALIESGKLSLSLEPSSLAEVMRECQAMIEPQAQ
                     KRGISVAFPSFDVPYFVNADRTRVKQVLINLLSNAIKYNKPGGTVVVDCVAGTPERIR
                     IGVTDTGEGLSPENLTQLFQPFNRLGQEANVEEGTGIGLMVCKRLIELMGGVIGVEST
                     VGKGSEFWFELNLTTDPHLATGATGPIVVDQAQVHADAPLRTLLYVEDNPAIRMLVED
                     LIARRPDVHLLTARDGVRGIEIARGCRPDVILMDINLPGISGITALKILAEDPATAHI
                     PVVALSANAAPRDIQKGLEAGFFRYLTKPIKIHEFMSTLDAALKIAGTNSARAGKEAN
                     A"
BASE COUNT         1191 a         2110 c         2106 g         1303 t
ORIGIN      
        1 caggcgcata acgattgcta tcgatgcgtg cgcttccgct cacggcgcga accgatgaga
       61 tacggtcgtt caagcccatc gcggctaacg acggataccg gcccgggcgc aggacgacgc
      121 agcggccgct gaaccggacg tcttcgcaga cctcccatcg gtcgcggaga accacgaccg
      181 acgaggcacg gtcattgaag ccaaagcgct cgaagttgcc gatctgtctt tcggtggtga
      241 aggtcctgcc ttggaagtct tcacgttcat agaaagtgac ctgtgcggtc gcttgcgttg
      301 cgatcgccac tgcggccatt gccaacgcat ttctcaacat cgcattcatg gatgcctcca
      361 ggtagggatt tgcgtctgcc ggagctgatg tcgaaccgaa gtatccaatt caacgctcga
      421 ctcaatcaga ctttgccggg agttgccccg actcagacac ccccactatg gcgcaacctc
      481 caacgggtgt ctgtgcggcg tcgtacgcag cgaaagcgtc tcagtgccat gcgcgagcaa
      541 cgcgggatgc gtccgcatga tcgacgcgta tgtgccccat cgcacagata gctttcgctt
      601 tccgcgtcat tcttcacagg tcgatcgaac atcggctctt ccgataccgt acttcaattg
      661 aaaggaaagc aaatgtccaa actgatgatt gcactacttg ccactgccgg actggggatt
      721 acggggcacg ctgcggcgca gacgtactcg tcgaagtcca cctcgacgcc gatgtcgaaa
      781 gacacctaca gtatggcgac gaagggtgcc gacgcgcagt acaagatcga caaggacgcg
      841 tgctcgtcgc tgagcgcaaa cgccaaggac atctgcctcg ccgaagccaa gggcaagcac
      901 aacgtcgcca aggctgacgc ggaagctgca tacaagcaca cgccaaaggc gcgcgaaaac
      961 gcgcgagtcg cgcgcgctca ggctaactac gatgtcgcta tagagaagtg cgacgacctg
     1021 gcaggcaacg tcaagaacgt ttgcgtgaag gaggcaaaag ccgaacttgt gaagggcaag
     1081 gccaacgcca aggtcgaccg cgtcgcagcc gatacgagcc aggacgcggc cgccaagcaa
     1141 gccgacgcgc gcaaggatgc gaacgcagac atgcgcgatg cagaatacaa ggtcgcgatc
     1201 gagaagtgcg acgcactggc gggtgcggcg aaggacggat gcgtgaacag cgcgaaagcg
     1261 aagttcggca aatcctgagt tcgatgcagg cctccctctc tgcctgcggg cggcgcgagg
     1321 gatcggtttt cgcggccggg gttgcttctt ccccggccgt tttttcgcgc agcggtcgcc
     1381 cgccgtccgc gcgccgtgct gccgactgat gcgaccgttc ggacgacaag ccgcgtacgt
     1441 ggcgacgcac ccgggcgctt tcgccctgcg tgtgctgcgc gcctttggtg ccaaccaggg
     1501 actgctgcta gctggagcca tcgcctacta cacgctgctg tccatcgtgc cgctgctgat
     1561 cctcgtgctg atcgcgctgt cccagatcgt cgcccagggc gagctgctcg ccaccttggg
     1621 ccgttatctg gaatggctga tgcccggaca gtcgacggcc gtgatcgcgg aactcgcccg
     1681 cttcctggac catcgcggcg tgatcggctg ggtgctgctg gcgacgatga tcttcttcag
     1741 ttcgctcgcc ttcagggtgc tggaaagcgc gatgtcggtg atcttcctgc accgggtggc
     1801 cgctcggcgg cgccgctttg tggtctcggc cgtgattccc tattgctaca tcgtggtgct
     1861 gggactgggc ctgctgctga tgacgctggt ttcgggttcg ctgcaggcaa tcggtgcgga
     1921 gagcatcgac tttctcggac gcaactggtc gctgcgggga ttctcgggcg cgctgctcta
     1981 cctgctgggc gtgatcggcg agattctcgt gctgacgtcg atctacctgg tgatgccggt
     2041 ggggcggccg tcgtggcgcc acgcgctcat cggcggcatc accgcaaccg cactgtggga
     2101 gatcacccgc cacgtgctgg tctggtactt cggcacgttg tcgcaggtca cggtcgtcta
     2161 tgggtcgctg acgacttcga tcgtcgtgct gctgagcctc gagatcgcgg cgactttatt
     2221 gctgctgggt gcgcaggtca tcgccgagta cgaacgcatt ggcacggacg gccaggacgc
     2281 ggcgccgccg ccgctatgga cgtaacacag cgtcgggtga tgtcgccgcg cgggggactg
     2341 tggtgtcagc gccttgccag ccgcctggcg tttgcagtgg ccttggcgtg aatcggcttc
     2401 aatccgcgct gcgcgagccg ggcggcactc ttcgacgcgc ctgccacgct ggtcgcagat
     2461 tgtcccagcg ctcgcgccag cgccgcttgc cgcgcaatca cctgcgccgg cgtgcggctc
     2521 gcggccaacg acatgaacgc cgccgtgctt cgttgcgcgc tctcgagcgc acgcgcgccc
     2581 gctggccgtc gcgcggtcat cagctgcgcg gccatcgctt gtgcggactg cgcgcctgct
     2641 tcgatctttt cctgccccat caatgcgaat tcgcgccggt cgcgcgcggt cggagcggca
     2701 cccgcccgcg ccatgcgtgc cgtgcgatgg ccgatgacct gtgccgacgc cagcatcatt
     2761 tccgcggtct tcatgaagag gctgttccag agcatgaacg gggccaagaa cggattgccg
     2821 aagtgattgc gcttcatttc tttccttgat ctggataacg gcataggcat gcgcggtcga
     2881 gcgatagtcc gtcaaatcgc gctcgcagtg atgccctggc gataccgcac atccgacagc
     2941 aagcggccga tttccttctt gacgacggca tagcactcgc aggcgccggc ttccaatccg
     3001 gaccgctcga gcactgcaat ctggccgcgg cggcagcgaa tcagaccggc acgctgcagt
     3061 tttccggcgg cctcggtaat gctttcccgg cgcacgccga gcgcactggc gaccagttcc
     3121 tgcgtcatga ccagctcatt cgagggcaat cggtcgaggg tcaacaacag ccatcggcac
     3181 aattgctgct cgatggaatg gtgccgattg caggccgtgg tctggcacat ctgcgtgagc
     3241 agcgcctgcg tatagcgcag caacaggcgc tgcatcagcc cggcgcgatt gaattcctcc
     3301 ttcagcaacc gtcccggcaa ccggtaaccg tggcccgccg tttgcaccac cgccgagctg
     3361 ggcgtggtgt caccgcccat gaacagggaa atgccgagga tgccttcgtt gcccacgcca
     3421 gcgatttcgg ccgatgcgcc ggactccagg acatagagca gcgacacgat ggcggtcgtg
     3481 ggaaagtaga cgtactgcaa ctggacgccg ggttcataga gactctcacc gagcagcatc
     3541 ggcaccagtt ccaggtgagg agccaggcgc tcgaattccg cggtgggcag ggcggcgaga
     3601 aggtggttct gattcgggct gtgcggcagc gacatgatca acgatcgaga ccgggtgggc
     3661 tattccctgc gatgagttcc acgccgattc cgcgataacc gatgaagcgg caggatcggt
     3721 tgaacatcgg ctctccgctc acctggaatc tctgctccga accatcggcg ttgactcggc
     3781 tgaagacgaa atcgaggaat ggctgccgcg ctgcgatcct ggcctgcagc tccgctcgct
     3841 ccgcttcatt ccagcccttg atctggtcat cgtctgcctc agccgcaaag gccgggaccc
     3901 ggattccgag catctcgagg accggaccgg agaccttggt gaagttcccg cgctcgtcct
     3961 gctcccagta ccaatcggag gccagctcgg tcaggctgcg atagcgcgct tcgctttcgc
     4021 gcagctcagc cgtccgttcc tgcaccgtcc gttccagcac ctggttgaac tcttcgagct
     4081 tccggtacaa gagccgcacc tccagcatgt tgcggatgcg cgtcttgact tcgatcagat
     4141 caaacggctt gctgatgaag tccttcgcgc cggcctgcaa cgcgcgcagc ttgtgacccg
     4201 gctgggccgt gagcacgatg acgggaagat agctgtcgga atcgctggtc ttgaggccct
     4261 ccatcacctg gaatccatcc atgcccggca tctgcaggtc gagcaggatc aggtcgtaac
     4321 ggtgcctgcg atgcagcgta aagacctccg ttggattcat cgtcgaggca acgcaggtgt
     4381 aaccggcatc actcagcaat tgctcgagca gctgcacgtt ggcttcctga tcgtcgacga
     4441 tcaggatgct ggcgttgagg atctcattgg cgctgaccat catgcgttcg cctccttgcc
     4501 tgcgcgggcc gagttcgtgc cggcgatctt cagcgccgcg tccagcgtac tcatgaactc
     4561 gtggatcttg atgggcttag tgagataacg gaaaaatcct gcctccagac ccttctggat
     4621 atcgcggggc gccgcattgg cactgagcgc gaccaccggg atgtgcgccg tggccgggtc
     4681 ttccgccagg atcttcagcg ccgtgatacc gctgatgccc ggcaggttga tgtccatcag
     4741 gatgacatcc ggccggcagc cgcgtgcaat ctcgatgccg cggacaccgt cccgcgcggt
     4801 cagcaagtga acgtcgggtc ggcgcgcgat cagatcctcg accagcatcc gaatggccgg
     4861 gttgtcctcg acataaagca gggtgcgcaa cggtgcatcg gcgtgaacct gtgcctggtc
     4921 gacaacgatg ggtccggttg cgccggtggc caggtgtgga tcggtcgtga ggttcagttc
     4981 gaaccagaac tcgctgccct tcccgaccgt gctttccacg ccgatgacgc ctcccatcaa
     5041 ctcgatcagc cgcttgcaca ccatcaagcc gatgccggtg ccttcttcga cgttcgcctc
     5101 ctggccgagg cgattgaacg gctgaaagag ctgcgtcaaa ttttccgggc tcagtccttc
     5161 gccagtgtcc gtgacgccga tgcgaatccg ctccggggtg cctgcgacgc agtcgacgac
     5221 cacggtgccg cccggcttgt tgtacttgat cgcgttggaa agcaggttga tgagaacctg
     5281 cttgacccgc gtccgatcgg cattgacgaa gtacgggaca tcgaacgagg ggaaggccac
     5341 gctgatgccg cgcttttgcg cctgcggctc gatcatggcc tggcattcgc gcatgacttc
     5401 ggcgagcgaa ctcggctcca gtgacagcga cagtttcccg gattcgatca gcgcgagatc
     5461 gaggacttca ttgatcagat ccagtagata ccagcccgcc ttgaggatct gatcgatgct
     5521 tcgcttctgg gagaccgtcg gcgacggcga acccgattcc atgagctggg cgaacccgag
     5581 gatcgcactg agcggcgtgc gcagctcatg gctcatgctg gacagaaagt ccgatttcgc
     5641 gaggttggcc ttttccgcgg cggccatggc gtcgtgcaat tccagctcga tctgcttgcg
     5701 cgcggaattg tcggcgccga tcaacaggta gccgatgatt tcgccatcgt cgttgcgcag
     5761 agcggtgatc gacacagtgg cgggaaagcg gctgccgtcc ttgcggatat acgtgagttc
     5821 gtagctgtct tcgatcccgc gcgacgcctt gaaggacagg gcctcgaagc ccggcgcaat
     5881 cggcgtcgcg agttcgaggc tcagtgcccg cgcgcgcgcc accacctcct gcggatcgtg
     5941 catgtcgctg gggctgatcc tgttcacgac ctcgggcgcg gaatagccca gcatgcgctc
     6001 ggcgccgatg ttgaatacct gaacgatgcc cttctcgtcg gtggcgatga tcgagaaact
     6061 ggcactggtg agaattgccc tctgcaaggc ccccgtcttc agcagcgttt cctggcgccg
     6121 cacctcggcg ctgcgatcgc cctcgcccgc agcttggaga atgacggaat cgctttcgaa
     6181 catggacggc cttcgtgtca acggaacgat gcggtggaag tgactttcgt tgccgagttg
     6241 gcgaggcgag agcaggaaca ccggtcgagt cgatgttcca tccgcaaaca tcgcctgctg
     6301 cgctcgcttg cggatcggct tcccgtgcgc ttgtcggcgc cagcaggaag caagcatggg
     6361 ccgcaagggg cggaataccc cggcgccacg ttcgcatcaa caaccaatga tcccgctgtt
     6421 tcggtcgaga atctgtacgc cagcgcacat agcccggccg gtccagcgag agagtccggc
     6481 ttgcgcgcgc cggccaactg ccggcggctg aatttcgcgc gcctcatctc gaggtattcg
     6541 cgttcgctca gttcgtgcaa ccgccttcag tattgctcgg tggcggcgaa ccagtcgtgc
     6601 acgcgtcgtt cgggcttctc gcgcgccggc aactcgacgg cgccaaggca agtatcgatc
     6661 gcaaggtagt aacgcgtgct gtttcactcg atcacgccgc gctcgcgctt
//
LOCUS       DBNHBY010000771         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_533390, whole genome shotgun sequence.
ACCESSION   DBNHBY010000771 DBNHBY010000000
VERSION     DBNHBY010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_533390"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="AC16HB_18115"
     CDS             complement(<1..1762)
                     /locus_tag="AC16HB_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR5608761.1"
                     /translation="MRAIARRERDEIERLWPKVMRRVGGYNLDVFHPQSERPYTRDGS
                     VNLAHLLVGSEGTLAYFKRLRLKLVPLPAQRVLGVVNFPTFYAAMDAAQHIVRLGPTA
                     VELVDRTMIDLARANPAFAPVIEAALIDHGGQPPDAILLVEFAGGDKSALVAQLARLV
                     ELMGELRLPDSVVRMTDDGAQKALWEVRKAGLNIMMSLRGDGKPVSFIEDCAVPLEHL
                     AEYTAQLTEVFRKHGTRGTWYAHASVGTLHVRPILDMRKDGAARMRAIAEEASAMVRR
                     YKGAYSGEHGDGLVRSEWVAWQFGPRLTAAFEEIKQLFDPRGLMNPGKIVRPTKMDDA
                     SLFRYRPGYAIGPLQPVLDWSAWDVHNDPATERLTAPGTGGDPARGFGKAVEMCNNNG
                     HCRKFDAGTMCPSFRATRDEQHVTRGRANTLRLALSGQLGPDAFTSDAVYEAMDLCVS
                     CKGCKRECPTGVDMAKMKIEFLHHYKLRHGHTLRDKLIAHLPRYAPWAARFPGLANLR
                     NRSPLLAGLGERWLGLSAQRSLPEFRADVFTRDASAAPAPARDRRQVVLFADTFNNYQ
                     EPDNLRAAQRVLEAGGYEAAV"
BASE COUNT          254 a          633 c          598 g          287 t
ORIGIN      
        1 cgacggctgc ctcgtacccg cccgcctcga gcacgcgttg cgcggcgcgc aggttgtcgg
       61 gctcctggta gttgttgaag gtgtcggcga acagcacgac ctgccggcga tcgcgtgcgg
      121 gcgcgggggc cgccgacgca tcgcgtgtga acacgtccgc gcggaactcc ggcagcgagc
      181 gttgcgctga aagacccagc cagcgctcgc cgagcccggc gagcagcggc gagcggttgc
      241 gcaggttggc cagtcccggg aagcgcgccg cccacggcgc atagcgtgga agatgcgcga
      301 tcagcttgtc gcgcagcgtg tgcccgtggc gcagcttgta gtgatgaaga aactcgatct
      361 tcatcttcgc catgtccacg ccggtcggac actcgcgctt gcagcccttg cagctcacgc
      421 acagatccat cgcttcgtac accgcgtcgg atgtgaacgc gtcggggccg agctgaccgc
      481 tcagtgccag ccgcagcgtg ttggcgcggc cgcgcgtcac gtgctgctcg tcgcgcgtgg
      541 cgcggaagct ggggcacatc gtgccggcgt cgaacttgcg gcagtggccg ttgttgttgc
      601 acatctcgac ggccttgccg aagccgcgcg ccggatcgcc gccggtcccg ggcgcggtga
      661 gccgctcggt cgcgggatcg ttgtgcacat cccacgccga ccagtcgagc accggttgca
      721 acggcccgat cgcgtagccc ggccggtagc ggaagaggct ggcgtcgtcc atcttcgtcg
      781 gccgcacgat cttgccggga ttcatcaacc cacgcggatc gaagagctgc ttgatctcct
      841 cgaacgccgc ggtcaggcgc gggccgaact gccacgcgac ccattcgctg cgcacgagcc
      901 cgtcgccatg ctcgcccgag taggcgccct tgtaccggcg caccatcgcg ctggcttcct
      961 cggcgatcgc gcgcatcctg gcggcgccgt ccttgcgcat gtcgaggatc ggccgcacgt
     1021 gcagcgtgcc gaccgacgcg tgcgcgtacc acgtgccgcg cgtgccgtgc ttgcggaaca
     1081 cttcggtgag ctgcgccgtg tactcggcca gatgctcgag cggcactgcg cagtcctcga
     1141 tgaagctcac cggcttgccg tcgccgcgca gcgacatcat gatgttcagg ccggccttgc
     1201 gcacttccca cagggccttc tgcgcgccgt cgtcggtcat ccgcaccacg ctgtccggca
     1261 gtctcagctc ccccatcagc tcgaccagcc gtgcgagttg tgcgacgagc gcggacttgt
     1321 cgccgcctgc gaactcgacc agcaggatcg cgtccggagg ttgaccacca tgatcgatca
     1381 gcgccgcctc gatcaccggc gcgaacgccg ggttcgcgcg cgccagatcg atcatcgtgc
     1441 ggtcgacgag ttccacggcg gtcggcccga gcctgacgat gtgctgcgcg gcgtccatcg
     1501 ccgcgtagaa ggtcgggaag ttcacgacgc ccagcacgcg ctgcgcgggc agcggcacga
     1561 gcttcagccg caagcgcttg aagtacgcca gcgtgccctc ggagccgacc agcagatgcg
     1621 cgagattgac gctgccgtcg cgcgtatacg gccgctcgct ctgcgggtgg aagacgtcga
     1681 ggttgtagcc gccgacgcgc cgcatcacct tcggccacag ccgctcgatc tcgtcgcgct
     1741 cgcgccgcgc gattgcgcgc accgcctcgc cc
//
LOCUS       DBNHBY010000772         2864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1209984, whole genome shotgun sequence.
ACCESSION   DBNHBY010000772 DBNHBY010000000
VERSION     DBNHBY010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1209984"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..524)
                     /locus_tag="AC16HB_18120"
     CDS             complement(285..524)
                     /locus_tag="AC16HB_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608762.1"
                     /translation="MQLRFSLALRSGKDAATPRNANTAISAAKANARRPTECISMTFP
                     QEESAWSGAAERHGPLSADRLDGADAARQAVGAGH"
     gene            complement(626..847)
                     /locus_tag="AC16HB_18125"
     CDS             complement(626..847)
                     /locus_tag="AC16HB_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608763.1"
                     /translation="MNAVKIAAIALIVAGVLGLVYGGFSYTKETHEVKMGPIELSVKE
                     KQNVNVPVWAGVGAIVIGGALLLFGGRRS"
     gene            complement(941..1087)
                     /locus_tag="AC16HB_18130"
     CDS             complement(941..1087)
                     /locus_tag="AC16HB_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR5608764.1"
                     /translation="MLETIAVVLIVLWILGLVSSYTMGGFIHALLVIAIVVILVRVIQ
                     GRGV"
     gene            complement(1264..1497)
                     /locus_tag="AC16HB_18135"
     CDS             complement(1264..1497)
                     /locus_tag="AC16HB_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR5608765.1"
                     /translation="MNWDRIEGNWKQITGKVKEQWGKLTDDDLDVIAGRRDRLAGKIQ
                     ERYGVAKDEVEKQLAEWERKAGDSWFIKAKSKV"
     gene            1877..2614
                     /locus_tag="AC16HB_18140"
     CDS             1877..2614
                     /locus_tag="AC16HB_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5608766.1"
                     /translation="MNPPIRRTFDPRQNQLLAALPDVDLHRWRPHLEFVELPFGQVLC
                     ESGSPHTHVYFPTTAIVSLMYMTQDGASAEIAVVGNDGVVGTSLFMDGAATPSQAVVQ
                     AAGYGFRLRAQAVKSELQRAGPALSTLLRYTQALIAQVAQTALCNRYHSIDQQLCRRL
                     LLGLDRSPSDELLMTQELVANLLGVRRGGVTAAALKLQQDGVIRYRRGHIVVLDRPRL
                     EQRSCECYAMAKKEYDRLLPLPLPLAA"
BASE COUNT          498 a          935 c          897 g          534 t
ORIGIN      
        1 gcatcggcgt ccgccgcgtt ccccttagga gcgttacggc gcggtatttg cgcgaaaccg
       61 caggcgtgtc gagaagcgct gtgcgtggcg ccgtcgagcg tccgcaggca aagcacgtgt
      121 agacgctcgg gcacaccgag gtgcgggcgt ttcttgccga gccggaccgg taccgcggct
      181 ttcgtgcaac ggtcatcgcg ctttgtccgc aacgccgatg ttcgagcctt ggcttaggag
      241 atccgcgggt cgcgtcggtt cggccctagt cagccgcgac ggattcagtg ccctgcaccg
      301 acggcctgcc gcgcagcatc cgcgccatcg agccgatctg cactgagcgg accgtgtcgc
      361 tcggccgcgc cggaccacgc gctttcttct tgcgggaacg tcatgctgat gcactctgtc
      421 ggtctgcgcg cattcgcctt cgccgcagat atcgccgtat tggcgttccg cggcgtggcc
      481 gcatctttgc ccgaccgcag ggcgagcgag aagcgcagct gcacgcgctg cacggcgggt
      541 cagaaaggcg tgcgaccgct gcgtccggaa gacgttggca actcaaaccc aatgtcgcgg
      601 gctgcaggcc tgcgctgaga gcacatcagc ttctgcggcc gccgaaaaga agcagcgcac
      661 cgccgatcac gatcgccccc acaccagccc agaccgggac gttgacgttc tgtttttcct
      721 ttaccgacag ttcgatcggt cccatcttga cctcatgcgt ttccttcgtg tagctgaatc
      781 ctccatacac caacccgagg acgccggcga cgatgagcgc gattgccgcg atcttgactg
      841 cgttcatttc gacttctccg attgaagaaa ttcttgttcc gcaacccgac tgcacgccgc
      901 gctgcccagc gccccgccga acaggcccct ttcgaattcg tcacacgcct cgaccttgaa
      961 taacccgaac caggatcacc acgatcgcaa tgaccaggag ggcatgaatg aatccgccca
     1021 tggtgtacga ggagacaagg cccagaatcc agaggacgat caagacgact gcaatggttt
     1081 caagcatcct gcttctcctt tcgacgtgac tcgcgaatcg caaatctggc gcctcgccgg
     1141 cgcgaaacga tgctcggtgt tcccccgacc gaggtccgcc gggaagcggc ggacctcaac
     1201 gagtgctgcg cgggattgcg gatctcactg gcggaccctg gctgcgatgt cgcccggtga
     1261 gaatcagacc ttgctcttgg ccttgatgaa ccacgagtcg cctgccttgc gctcccactc
     1321 ggccagctgc ttttcgacct cgtctttggc cacgccgtag cgctcctgga tcttgccggc
     1381 gagacggtcg cggcggccgg cgatgacgtc gaggtcgtca tcggtcagct tgccccattg
     1441 ctccttgacc ttgccggtga tttgtttcca attgccttcg atgcggtccc agttcatgtg
     1501 aatccctttc attgatgcgt tgagaagtcg tgaacgcaag ctcgcgccga gctccggagc
     1561 agatggcgat ctcgtatttg acccacctca tctccccggc gacaggattg cacttcaacc
     1621 gccgcggagt cggtgcgcta tcgaacccat cgcgggatca agtaaccgcg ccgacatggc
     1681 gtgctgcaaa tgcgtgaccg agttgctctc caacgttgca gatgccggac ggaaccgccc
     1741 atgcgcgcgg cgattccccg gtgctggacc gcgcagcgct gtaggacggt gccgacatcc
     1801 gaacccgatc gcctccgata cctgaggccg cagcgaatct ggatcatggg gtctcgagga
     1861 acgctgagag gccccaatga atcctccaat ccgtcgcacg ttcgacccgc gccagaacca
     1921 gttgttggcc gcactgcctg acgtcgatct gcatcgctgg cggccgcacc ttgagtttgt
     1981 ggaactgcct ttcggccagg tgctgtgcga atcgggcagc ccgcacacgc atgtgtactt
     2041 tccgaccacg gccatcgttt cgttgatgta catgacgcag gacggtgcgt cggccgagat
     2101 cgcggtcgtc ggcaacgacg gcgtggtggg cacgtcgctg ttcatggacg gcgccgcgac
     2161 gccgagccag gccgtggtgc aagccgcggg gtatgggttc cgattgcgtg cgcaggcggt
     2221 caagagtgag ctgcaacgtg cgggcccggc actgagcacc ctgctgcggt acacacaggc
     2281 cctcatcgcg caggtggcgc agacggccct ctgcaatcgc taccactcga tcgatcagca
     2341 attgtgccgt cgactgctgc tgggactgga ccgatcgccg tccgacgaac tgctgatgac
     2401 gcaggaactg gtggccaacc tactgggcgt gcgccgagga ggcgtgaccg ccgcggccct
     2461 caagctgcag caggacggcg tgattcggta caggcgcggc cacatcgtcg tgctggatcg
     2521 cccgcgcctc gaacagcgct cttgcgagtg ctacgcgatg gccaagaagg aatatgaccg
     2581 gctcctcccg ctgccgctgc cactcgccgc atgaagacgc tccgcggtgc cgtgcgcgcg
     2641 atcggaaatc gcgggcattc accctgcacc aggaacatga gggccacgtg ttgtggcatc
     2701 ggggccgcga ggggcgcgga cagcgttcac gctgccgaca cgctcacgat ggcgtggtgt
     2761 cgcggaacaa tccggcgcgt tcaaccagat ggatcatctg cgcggccgcg cggatcgtca
     2821 gtcgatgggg ggtgaagcct ggcctgggag agatgtagga cgcc
//
LOCUS       DBNHBY010000773        13048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_330596, whole genome shotgun sequence.
ACCESSION   DBNHBY010000773 DBNHBY010000000
VERSION     DBNHBY010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_330596"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /gene="coaE"
                     /locus_tag="AC16HB_18145"
     CDS             <1..475
                     /gene="coaE"
                     /locus_tag="AC16HB_18145"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696565.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5608767.1"
                     /translation="FGSEFIDSNGALDRSRMRELVFRDASARRSLERVLHPMIRARAE
                     SFAAAAPAPTPYVVFVIPLLIESGSWRTRADRVLAVDCAIDTQVTRVMLRSGLAESAA
                     RAIVASQATRAARLDAADDVVVNEGTPADARRCAARLHALYAALRPPADNQGRSL"
     gene            503..1276
                     /gene="zapD"
                     /locus_tag="AC16HB_18150"
     CDS             503..1276
                     /gene="zapD"
                     /locus_tag="AC16HB_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIR5608768.1"
                     /translation="MSNEHRPILYEYPFSERLRTLLRLEDLFDKLDYFVGQTHPHCHH
                     TALLTLFEILEVTSRADLKSDLLQELERHKQTLLAFRNNPQVSESALTSVLGELERAQ
                     HALNATPGKAGQHLRDNEWLTSIRSRAGIPGGTCEFDLPSYHAWLMRDADARTQDLQA
                     WIRPMQQFNMGLSIVLRLLRETAQRSRQVAAQGSYQQTLQGRSYSLLQVRIAADLGAI
                     PEISANKYMLWIRFTAADRDLKPRPIDRDVPFELALCAF"
     gene            1294..1494
                     /locus_tag="AC16HB_18155"
     CDS             1294..1494
                     /locus_tag="AC16HB_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR5608769.1"
                     /translation="MSKTVACPVCGAAVPWTPSSTWRPFCSERCKTIDLGDWASERHV
                     IAGRPDVNHDSATRDDGTARDH"
     gene            complement(1510..2463)
                     /locus_tag="AC16HB_18160"
     CDS             complement(1510..2463)
                     /locus_tag="AC16HB_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HIR5608770.1"
                     /translation="MIAPAVGRPVVQVAVGVLMHTDGAVLLADRPEGKPYAGFWEFPG
                     GKIEAGETVAAALARELHEELGIDLGPSLPWVVFEHDYPHAYVRLHFRRVFEWHGEPH
                     PREGQHFGFFLPEQAPQPLLPAAVPALRWLSLPTIYGVSNIAAMGAGNFTRAFDRALV
                     RGLRLLLVREPTLDDRQLGTLAPALLAHAHANGARVLISSRHGESLRAAFDGVHLTAR
                     DLMYTTQRPSAGLVAASVHSRQELAHAHRLGCDFAMAGAVRPTPSHPGAVPLGWDGFA
                     AIARESPLPLYAIGGLAPGDLADAMRAGAHGIALLRAAWGD"
     gene            complement(2460..3353)
                     /locus_tag="AC16HB_18165"
     CDS             complement(2460..3353)
                     /locus_tag="AC16HB_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5608771.1"
                     /translation="MTRKGAEQARLLELLERLAPLLPQARPIDWSAPAFRWRRRQFLG
                     ASLGRLEAIHRIARIAPEDLQHVEAQKAAILRNTEQFVRGAPANNVLLTGARGTGKSS
                     LIRACLVAFMDQGLRLIEVDKRDLVDLPELMELIVDRTERFVVFCDDLSFEEGEASFK
                     ALKAALDGSVAAPGENLLIYATSNRRHLMPEYMRENLAAHYEDDGEIHPAETVEEKIS
                     LSERFGLWLSFYPFRQDDFLDIVRHWLRRFEIDVADPAHASWREEALQFALQRGSRSG
                     RVALQFARDFAGRARLSTPRR"
     gene            complement(3350..4576)
                     /gene="argJ"
                     /locus_tag="AC16HB_18170"
     CDS             complement(3350..4576)
                     /gene="argJ"
                     /locus_tag="AC16HB_18170"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR5608772.1"
                     /translation="MAVGLEAPRAEALMPVAGVELGSAEAGIRKANRKDLLVIRVAPG
                     ASVAGVFTQNRFCAAPVQVCREHLAADTEVRALVINTGNANAGTGAPGVAAARATCAE
                     LAGRLGCTPSQVLPFSTGVIMEPLPLDRLLAGLPAAIGRLAADQWLPAAESIMTTDTV
                     PKAVSARINLDGTPVTITGIAKGAGMIRPDMATMLGFVATDAGVAPALWRRLVRRVAD
                     ASFNRITVDGDTSTNDSFVMIATGRAGARIDDERDSHLPQLADALTRVAQQLAQAIVR
                     DGEGATKFIEIEVLAARTPAEAAQIAYSIAHSPLVKTAFFASDPNLGRILAAVGKAGV
                     ADLDQSRVQLWLDDVWVARDGGRHPEYREEDGQRVMRADDIRVRVALGRGDASATVWT
                     CDLSHDYVSINADYRS"
     gene            complement(4587..7346)
                     /gene="secA"
                     /locus_tag="AC16HB_18175"
     CDS             complement(4587..7346)
                     /gene="secA"
                     /locus_tag="AC16HB_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5608773.1"
                     /translation="MISGILTKIFGSRNQRLLKQYQRVVERVNAFEAGIAALDDAQLQ
                     AKTAEFRTRYANGETLDQLLTEAFAVVREAAKRALGMRHFDVQLIGGMVLHNGKIAEM
                     RTGEGKTLAATLPAYLNALSGKGVHIVTVNDYLASRDADWMGKVYRFLGMSVGVILSQ
                     QSTEDKRAAYAADITYGTNNEFGFDYLRDNMEYSVADKRQRGLNYAIVDEVDSILIDE
                     ARTPLIISGPAEDHTELYVRMDEVPKLLTRQADEKGSGDFWVDEKGHQIHISEEGHEK
                     VEDILVKMGLLGEGESLYAPQNIVLMHHLNAALRAHHLFHRDQHYVVQNNEVIIVDEF
                     TGRLMAGRRWSEGLHQAVEAKEHVPIQQENQTLASITFQNYFRMYGKLAGMTGTADTE
                     AYEFQEIYGLETVVIPTHKPMVRKDHQDKIYRTINEKYAAILEDITDCHGRGQPVLVG
                     TTSIENSEALSQLLTKSKLPHQVLNAKQHAKEAEIVAQAGRPGVITIATNMAGRGTDI
                     VLGGNVEKQAQFVEANPDLSPEDKKARIETLRGEWQSLHNQVVDAGGLKIIGSERHES
                     RRIDNQLRGRSGRQGDPGESRFYLAMDDPLLRIFAGDRMRAIMDRLKLPEGEAIEAGM
                     VTRSIESAQRKVEARNFDIRKQLLEYDDVANDQRREIYRLRNEILEAKDTREMVDGLR
                     SGYFTDMFREFVPAESVEEQWNIDGLTTALASDWQIDLPLKQWLENEDLNDVDLLERI
                     LTATNEAYAAKVALVGSEAFGGFERSIMLQVLDQHWREHLAALDHLRQGIHLRGYAQK
                     NPKQEYKREAFELFGTLIDRVRADVIRILMNVRIQTQEEIAAAEQKIEEESERRLEHA
                     RAQHAEFATAVAEEAADVEALTLPPPEEAKAQPFKRFGDKIGRNDPCPCGSGKKYKQC
                     HGKLA"
     gene            complement(7457..8458)
                     /locus_tag="AC16HB_18180"
     CDS             complement(7457..8458)
                     /locus_tag="AC16HB_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5608774.1"
                     /translation="MQIIVVDPRFARAVTLTLTQRTFWVVLTLTALTFVFSVTGLYVA
                     TLKAVSEFRIPYLHDLTATVMRDEVARNEQFMRDNVSAMARKLGEMQAQLMRLDALGE
                     RVSRLAGIRPEEFNFRELPGRGGADTPGRPLTMDELKSELDRVARGVEHRADYMDVIE
                     SELMSARVRRTLLPENTPVSEGFVGSGYGMRTDPITGQLAMHAGLDFAAPIGTPIFAA
                     AGGVVVSAEYHPMYGNAVTVDHGNHLSTLYAHASRIVVKPGDIVRKGQKLAEVGTTGR
                     STGPHLHFEVHVNGAPQNPARFLARQAKDSPLAQLGGSQKSVRAIDVRGLATTGADR"
     gene            complement(9018..9194)
                     /locus_tag="AC16HB_18185"
     CDS             complement(9018..9194)
                     /locus_tag="AC16HB_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608775.1"
                     /translation="MPYFRALLFVFAATIIVLVALSLFSGDRKYLRWTGRLFGIGIAG
                     AVLFFVVLLIQRFI"
     gene            complement(9207..10127)
                     /gene="lpxC"
                     /locus_tag="AC16HB_18190"
     CDS             complement(9207..10127)
                     /gene="lpxC"
                     /locus_tag="AC16HB_18190"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR5608776.1"
                     /translation="MLKQRTLKTLVRTVGIGMHSGTKVELVLRPAAADTGIVFRRIDL
                     DPVVDIPVRPEAIGDTRMASTLVQNGARVATIEHLMSALAGLGIDNAFVDVDAAEVPI
                     MDGSAASFVFLLQSAGIVELSAPKRFIRVRRRVEVRAGAGADEKWARLEPYDGYKLRF
                     SIDFAHPAIDATDQDVEVDFARDSYVTAVARARTFGFVNEVEALRAAGLGLGGNFQNA
                     IVMDEYRVLNTDGLRSGDEFAKHKILDAMGDLYVLGRPLIAAYVAHKSGHALNNQLVR
                     ALLADKAAYELVVFEDAAAAPAGLRQPVPA"
     gene            10214..10993
                     /locus_tag="AC16HB_18195"
     CDS             10214..10993
                     /locus_tag="AC16HB_18195"
                     /inference="COORDINATES: protein motif:HMM:NF021472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HIR5608777.1"
                     /translation="MNEPAPLMPSASPFPALRTVSFGAPLGWLSRAATDLRAAPSASL
                     FYGAAFASMGWAIYFVFRHAYEYASALTAGFLLLGPFLCTGLYDISRRVEHGGSAHLA
                     PTLTAWRANLGAFSLFALILTVLMLIWARASLVTFALFFSSGMPTLSGFLEQVVSVEH
                     WDFVLTWLAVGAIFAAIVFAVSVVSVPMMLDRGTDTVVAALTSVRALLANPGPLLAWA
                     ALIVLLIGVGFATAFIGLVITAPLVGHATWHAYREIVVRDR"
     gene            complement(11047..12237)
                     /gene="ftsZ"
                     /locus_tag="AC16HB_18200"
     CDS             complement(11047..12237)
                     /gene="ftsZ"
                     /locus_tag="AC16HB_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5608778.1"
                     /translation="MAFEILETETRGTVIKVVGVGGAGGNAIEHMIRRGVQGVEFICA
                     NTDHQALARSAAKNIIQLGTTGLGAGSRPEAGRQSAIDGRDRIADALRGAHMVFITAG
                     MGGGTGTGAAPVVAEIAKELGILTVAVVSKPFEFEGPRRMRVAEDGLGELQERVHSLI
                     VILNNKLEEVLGEDASFEECFRAADDVLHNACAGIAEIINIPGLVNVDFEDVKTVMSE
                     HGKAMMGTATAAGLDRARIAAEQAVASPLLEGVDLSGARGVLVNITASNSLRMKETRE
                     VMNTIRAFAAEDATVIFGTVCDESMGENLRVTVVATGLGRTSTAKKQTPFTVIKTGTH
                     DTPVVMPPVAHANGGASSGDYSHFDTPAVWRSRESAQARVSAMEDAGHDRFDIPAFLR
                     KQAD"
     gene            complement(12339..>13048)
                     /gene="ftsA"
                     /locus_tag="AC16HB_18205"
     CDS             complement(12339..>13048)
                     /gene="ftsA"
                     /locus_tag="AC16HB_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5608779.1"
                     /translation="RCGLEVQDLILQPLASSLSVLTPDEKELGVALVDIGGGTSDIAI
                     FTGGAIRHTAVIPIAGDQITNDIAMALRTPIPDAEEIKLRSGVAKEVLADPNDRIEIP
                     GIGDRAPRLLSRQALAAVIEPRVEELFTLTQQVVRDSGYEELLSSGVVLTGGTSLLPG
                     VGELAEDVFLKPARIGWPTYDGALADVIRNPRFATVMGLLTEAHLQRVRGRKVAAQSG
                     SFKQTLKRMKEWIVGNF"
BASE COUNT         2115 a         4495 c         4139 g         2299 t
ORIGIN      
        1 cttcggctcg gagttcatcg acagcaacgg cgcactcgat cgcagccgca tgcgcgaact
       61 cgtgttccgc gacgccagcg cgcgcagatc actcgaacgg gtcttgcacc caatgatccg
      121 agcccgcgcc gagtcgtttg ccgccgccgc accggcgccg accccttacg tcgtcttcgt
      181 aattccgctg ctgattgagt cgggatcgtg gcgcacgcgc gccgatcgcg tgctcgcggt
      241 cgactgcgcg atcgacaccc aggtcacccg agtcatgctt cgcagcggtc tggcggaaag
      301 cgccgcgcgc gcgatcgtcg ccagtcaggc gacgcgcgcg gcgcgccttg acgccgccga
      361 cgacgtggtg gtgaacgaag gcacgccggc ggatgcgcga cgctgtgccg cgcgcctgca
      421 tgcgctgtat gccgcgctgc ggccacccgc tgacaaccag ggcaggtcgc tgtgatatct
      481 tcgcgcccaa cagcgcgtcg ccatgagcaa cgaacaccgg ccgatcctct acgaataccc
      541 gttcagcgaa cgcctgcgga ccctgctgcg tctggaagat ctgttcgaca agctcgatta
      601 tttcgtcggc cagacgcatc cgcactgtca tcacaccgcg ctgttgacgc tgttcgagat
      661 tctcgaagtc acatcgcgcg ccgacctgaa gagcgatctg ttgcaggagc tcgagcgcca
      721 caagcagacg ctgctcgcgt ttcgtaacaa cccgcaggtg tccgagtcag cactgacgtc
      781 cgtactcggc gaactcgagc gcgcgcagca tgcgctgaac gccacgcccg ggaaggcggg
      841 gcagcacctg cgcgacaacg agtggctgac cagcatccgc agccgcgccg gcatcccggg
      901 aggcacatgc gagttcgatc tgccgtcgta ccacgcgtgg ctgatgcgcg atgccgacgc
      961 gcgcacgcag gacctgcagg cctggatccg ccccatgcag cagttcaaca tggggctgtc
     1021 gatcgttctg cggctcctac gtgagactgc acagcgcagc cggcaggtcg ccgcgcaggg
     1081 ttcgtaccag cagactctgc aggggcgcag ctactcgctg ctgcaagtgc gcatcgccgc
     1141 cgatctcggc gcgattcctg aaatcagcgc gaacaagtac atgttgtgga tccgcttcac
     1201 cgccgccgat cgcgacctca agccgcgccc gatcgaccgc gacgtgccgt tcgagttggc
     1261 gctgtgcgcg ttctgagatc gaccgcggac gcgatgtcga agaccgtcgc ctgccccgtg
     1321 tgcggggccg ccgtgccctg gacaccgagc agcacgtggc gcccgttctg ctccgagcgc
     1381 tgcaagacga tcgatctcgg cgactgggcg agtgagcgac acgtgattgc cggccggcct
     1441 gacgtcaacc acgattccgc cacgcgcgac gatggcacgg cgcgcgacca ttgagcgcac
     1501 ggcttacgcc taatcgcccc aagccgcgcg cagcagtgcg atgccgtgcg cgccggcgcg
     1561 catggcgtcg gcgagatcgc cgggggccag cccgccgatg gcatacagcg gcaacggcga
     1621 ctcacgcgcg atcgcggcga atccgtccca tccgagtggc accgcacccg gatggctcgg
     1681 ggtcgggcgc actgcacccg ccattgcgaa atcgcagccg agccgatgcg cgtgcgcaag
     1741 ctcttgacgg ctgtgcaccg acgccgcgac gagtccggca ctcggccgct gcgtcgtata
     1801 catcaggtcg cgcgccgtca ggtgcactcc gtcaaaggcc gcccgcagcg attcgccgtg
     1861 gcggctgcta atcagcacgc gcgcgccgtt cgcgtgcgcg tgcgcaagca gcgccggcgc
     1921 cagcgtgccg agttgacgat catccaatgt gggttcgcgc accagcagca ggcgcaggcc
     1981 gcgcaccagc gcgcggtcga aagcgcgcgt gaagttgccc gcccccatcg ctgcgatgtt
     2041 cgaaacgccg tagatcgtcg gcaggctcaa ccagcgcagc gccgggacgg ccgcgggcag
     2101 cagaggctgc ggcgcctgct caggcaggaa gaagccgaag tgctgcccct cgcggggatg
     2161 cggttcaccg tgccactcga acacgcggcg aaagtgcagc cgcacatagg cgtgcggata
     2221 gtcgtgttcg aacacgaccc atggcaacga cgggccgaga tcgatgccga gctcctcgtg
     2281 caattcgcgc gcgagcgccg ccgcaaccgt ttcacccgcc tcgatcttgc cgcccgggaa
     2341 ctcccagaag ccggcatacg gcttgccttc ggggcgatcg gcgagcagca ccgcgccgtc
     2401 ggtgtgcatc aggacaccga cggcgacctg aacgactggg cgcccgacgg cgggcgcaat
     2461 catcgccgcg gggtcgagag ccgcgcgcga ccggcgaagt cgcgtgcgaa ctgcagcgcc
     2521 acgcggcccg agcgcgaacc gcgctgcagg gcgaactgca gcgcctcctc gcgccagctc
     2581 gcgtgagctg gatcggcaac gtcgatttcg aagcgccgca gccagtgacg cacgatatcg
     2641 aggaaatcgt cctggcggaa cggatagaac gacagccaca ggccgaaacg ctccgaaagc
     2701 gagatcttct cctccacggt ctcggccgga tggatctcgc catcgtcttc gtagtgcgcg
     2761 gcgaggttct cgcgcatgta ctcgggcatc aggtggcgcc ggttggacgt cgcgtagatc
     2821 agcaggttct cgccgggcgc tgcgaccgag ccgtcgagcg cagccttgag cgccttgaac
     2881 gatgcctcgc cctcttcgaa cgacaggtcg tcgcagaaca ccacgaagcg ctcggtgcgg
     2941 tcgacgatca gttccatcag ttcgggcagg tcgacgagat cgcgcttgtc gacctcgatg
     3001 agccgtagcc cctggtccat gaacgccacc aggcaggcgc ggatcagcga cgatttgccg
     3061 gtgccgcgcg cgccggtcag cagcacgttg ttagccggcg cgccgcgcac gaactgctcg
     3121 gtgttgcgca ggatcgcggc cttctgtgct tcgacgtgct gcagatcttc cggagcgatg
     3181 cgcgcgattc ggtgaatcgc ttccagccgg ccgagtgacg cccccaggaa ttgccggcga
     3241 cgccagcgaa atgcgggcgc gctccagtcg atcggccggg cctgcggcag taacggcgcg
     3301 agccgctcca gcagttcgag cagtcgcgcc tgctcggcgc ctttcctcgt catgaacgat
     3361 agtcggcgtt gatcgacacg tagtcgtgcg acaggtcgca tgtccacacc gtggccgagg
     3421 catcgccgcg acctagcgca acgcgcacgc ggatgtcgtc ggccctcatc acgcgctggc
     3481 cgtcctcctc gcgatattcg ggatgacggc cgccgtcgcg cgcgacccac acgtcgtcaa
     3541 gccacagctg aacgcggctc tggtcgaggt ccgccacgcc cgccttgccg accgcagcga
     3601 ggatgcggcc gaggttcggg tcagaggcaa aaaacgcagt cttcaccagc ggcgaatgcg
     3661 caatcgagta tgcgatctgc gcggcctctg ccggcgtgcg tgccgcaaga acctcgattt
     3721 cgatgaactt ggttgcgccc tcgccatcgc gcacgatcgc ttgcgccagt tgctgcgcaa
     3781 ctcgcgtcaa tgcgtcagcc agctgcggca ggtgcgaatc gcgctcgtcg tcaatgcgcg
     3841 cgccggcgcg gccggtcgcg atcatcacga acgaatcgtt ggtcgacgtg tcgccgtcga
     3901 cggtgatgcg attgaacgat gcatcggcca cccgccgcac cagtcgccgc cacagcgcgg
     3961 gcgccacgcc ggcgtcggta gccacaaacc ccaacatcgt cgccatgtcg gggcggatca
     4021 ttccggcgcc cttcgcgatg cccgtgatcg tgacgggtgt gccgtcgaga ttgatgcgcg
     4081 ccgataccgc cttcggcacc gtgtcggttg tcatgatcga ctcggctgca ggcagccact
     4141 gatcggccgc gagccgaccg atcgccgcgg gcaggccggc gagcagtcga tcgagcggca
     4201 gaggttccat gatcacgccg gttgaaaatg gcaacacctg cgacggcgtg cagccgagcc
     4261 gaccggcgag ctcggcacag gtcgcgcgtg cggcggcgac gcccggcgcg ccggtacccg
     4321 cattggcatt gccggtattg atgaccagcg cgcggacctc ggtgtccgcg gcgaggtgct
     4381 cgcggcacac ctggaccgga gccgcgcaga accggttctg cgtgaatacg ccggccacgc
     4441 tcgcaccagg cgcaacgcgg ataaccagca ggtccttgcg attggccttg cggattcccg
     4501 cttcggccga accgagttcg acgccggcaa ccggcatcaa tgcttcggcg cgtggcgctt
     4561 cgagtccgac tgccatcgcg gcacgctcaa gccagcttgc cgtgacattg cttgtacttc
     4621 ttgcccgagc cgcacgggca cgggtcatta cggccgatct tgtcgccaaa gcgcttgaac
     4681 ggctgcgcct tcgcctcttc cggcggcggc agcgtgagcg cctcgacgtc ggccgcctcc
     4741 tccgccaccg ccgtggcgaa ttcggcgtgc tgcgcgcgcg cgtgctccag ccgccgctcc
     4801 gattcttcct cgatcttctg ctctgcggca gcgatttctt cctgcgtctg gatccggacg
     4861 ttcatcagaa tgcggatcac gtcagcgcgc acgcgatcga tcagcgtacc gaacagctcg
     4921 aacgcctcgc gcttgtattc ctgcttcgga ttcttctgtg catagccgcg caggtggata
     4981 ccctggcgca ggtggtcgag cgcagcgagg tgctcgcgcc agtgctggtc cagcacctgc
     5041 agcatgatcg accgttcgaa gccgccgaat gcttcgctgc ccaccagcgc caccttcgcc
     5101 gcgtacgcct cgttggtcgc cgtcaggatc cgctcgagca gatcgacgtc gttcagatcc
     5161 tcgttctcga gccactgctt cagcggcaga tcgatctgcc aatcggaggc gagcgcggtg
     5221 gtcaggccgt cgatgttcca ctgctcctcg accgactcgg caggaacaaa ttcgcgaaac
     5281 atgtccgtga agtatccgct ccgcaggcca tcgaccattt cacgcgtgtc cttcgcctcg
     5341 aggatctcgt tgcgcagccg gtagatctcg cgccgctggt cgttggcgac gtcgtcgtat
     5401 tcgagcagtt gcttgcggat gtcgaagttg cgggcctcga ccttgcgttg cgccgactcg
     5461 atcgaacgcg tgaccatgcc ggcctcgatc gcttcgccct cgggaagctt gagccggtcc
     5521 atgatcgcgc gcatccggtc gccagcgaag atgcgcagca gagggtcgtc catcgccagg
     5581 tagaaacgcg attcgcccgg atcgccctgg cgtccggagc gaccgcgcag ctggttgtcg
     5641 atgcggcgcg attcgtgccg ctcggatccg atgatcttca atccgccggc gtcgacgacc
     5701 tggttgtgca gcgactgcca ttcgccgcgc agcgtctcga ttcgcgcctt cttatcctcg
     5761 ggcgagaggt cggggttcgc ctcgacgaac tgcgcctgct tctcgacatt gccgcccaac
     5821 acgatgtcgg taccgcggcc agccatgttg gttgcaatcg tgatgacgcc gggccggccc
     5881 gcctgcgcga cgatctctgc ctctttcgcg tgctgcttgg cgttgagcac ttggtgcggc
     5941 agcttcgact tcgtcagcag ctgcgaaagc gcctcggagt tctcgatcga agtcgtgccg
     6001 accaggacgg gctgaccacg cccatggcaa tccgtgatgt cctcgagaat cgcggcatac
     6061 ttctcgttga tggtgcgata gatcttgtcc tgatgatctt tgcggaccat cggcttgtgg
     6121 gtcggaatga cgaccgtctc caagccgtag atctcctgga actcgtatgc ctcggtatcg
     6181 gcggtacccg tcatcccggc gagcttgccg tacatgcgga aatagttctg aaacgtgatc
     6241 gatgccagcg tctggttctc ttgctggatc ggcacgtgct ccttggcttc gaccgcctgg
     6301 tgcaatccct cggaccagcg ccgcccggcc atcagccggc cggtgaattc gtcgacgatg
     6361 atcacctcgt tgttctgcac cacgtagtgc tggtcgcggt ggaaaaggtg gtgcgcgcgc
     6421 agcgccgcgt ttaggtggtg catgaggacg atgttctgcg gcgcatagag gctctcacct
     6481 tcgccgagca gccccatctt gaccaggatg tcctccacct tctcgtgccc ctcttccgag
     6541 atgtggatct ggtgcccttt ctcgtcgacc cagaagtcgc cgctgccctt ctcgtccgcc
     6601 tgccgtgtca gcagcttcgg cacttcgtcc atccgcacgt acagctcggt gtgatcctcg
     6661 gccggcccgg aaatgatcag cggcgtgcgc gcctcgtcga tcaggattga gtcgacctcg
     6721 tcgacgatcg catagttcag cccgcgctga cgcttgtcgg cgaccgagta ctccatgttg
     6781 tcgcgcaggt agtcgaagcc gaattcgttg ttggtgccgt acgtgatgtc ggccgcgtac
     6841 gcggcgcgct tgtcctcggt cgactgctgc gacaggatca cgccgacgct catgccgagg
     6901 aaccggtaga ccttgcccat ccaatcggcg tcgcgactgg ccaggtaatc gttgacggtc
     6961 acgatgtgca cgcccttgcc cgacagcgca ttcaggtacg cgggcagcgt cgccgccagc
     7021 gtcttgccct cgccggtgcg catctcggcg atcttgccgt tgtgcagaac catgccgccg
     7081 atgagttgca cgtcgaagtg acgcatgccg agcgcgcgct tggcggcctc gcgcacgact
     7141 gcgaaggcct cggtcagaag ctgatcgagc gtctcgccgt tggcgtagcg ggtacggaat
     7201 tcagcggtct tggcctgcag ctgcgcgtcg tcaagcgcag cgattcccgc ctcgaacgcg
     7261 ttcacgcgct ccaccacgcg ctggtactgc ttgagcaggc gctgattgcg gctgccgaaa
     7321 atcttggtga ggatgccgga gatcatcagg atgggatcgg aaccgcggcg cgtcgcggca
     7381 gcgcggcgcc cgcttcgcgc acctgcgcga agcggcggat tatctcacgg ggggaaacgc
     7441 ggcccgcagg tgggaactat cggtcggcgc cggttgtggc cagcccgcgc acatcgatcg
     7501 cgcggaccga tttctgcgag ccgcccagtt gcgccagcgg cgagtccttc gcctggcggg
     7561 ccaggaagcg cgccggattc tgcggcgcgc cgttgacgtg cacttcgaag tgcaggtgcg
     7621 gaccggtcga acgtccggtc gtgccgactt cggccaactt ttgtcccttg cggacgatgt
     7681 cgcccggttt gacgacgatg cggctggcgt gcgcgtacag agtgctcagg tggttgccgt
     7741 gatcgacggt cacggcgttg ccgtacatcg ggtgatactc ggcgctgacc acgactccgc
     7801 ccgccgcagc gaagatcggc gtcccgatgg gcgcggcgaa atcgagtccg gcatgcatcg
     7861 ccagctggcc agtgatcggg tcggtccgca tcccgtagcc cgagccgacg aagccctccg
     7921 aaacgggcgt gttctccggc agcagcgtgc gccgaacgcg cgccgacatc agctcggact
     7981 cgatcacgtc catgtagtcg gcacggtgct cgacgccgcg cgcgacacga tccagttcgg
     8041 acttcagttc atccatcgtc agcggtcggc ccggcgtgtc ggcgccaccg cggccgggaa
     8101 gctcccggaa attgaactcc tccggccgga taccggccag ccgcgataca cgctcgccga
     8161 gcgcatccag ccgcatcagc tgtgcctgca tctcgcccag cttgcgcgcc atggccgaca
     8221 cgttgtcgcg cataaactgc tcgttgcgcg cgacttcgtc tcgcatcaca gtggccgtca
     8281 ggtcgtgcag atacggaatt cggaactcgc tgacggcctt gagcgtggcc acgtacaggc
     8341 ccgtcaccga gaacacgaac gtcagcgccg tcagcgtcag cacgacccag aatgttcgct
     8401 gcgtcagggt cagcgtcacg gcacgcgcga aacgcggatc gaccactatg atctgcacaa
     8461 gtcaccctcc ggcacgttga cgcaaacggg ctcgataatc tgctccggct acgccaaggc
     8521 tcgtttcttg tatttcagac agatgcactc cggcaaaacc ctcgtccaag cgctcgccga
     8581 gaccccggcc gccgccctga tctcacgctg cgagcgcgcc gaccgcgccg cccgcctgat
     8641 ccttgcagca ctgggcgaat cgacaaccta cccgggccaa ctcccgacca tccaatgcca
     8701 gatcagggag cacgtacttc tggtctttgc cagttcagta gcacaagcgg ccaagttacg
     8761 gcaggcgctg ccgcgattcc ttgcgctact tcaagaacgc gggttcaacc ttagcgaaat
     8821 ccggattcgg gttcaacccg gctcctcggc tgagcccaaa tggggggcgt ccgtcccccg
     8881 tccgataacg tcgaattcag ctcaacttcg gccatcttcg gccggcgcgc tccaattcgc
     8941 ggacgagctc gcgcttgcct tgaaaccttc gccgctgcga gacgcggcgg aacggctggc
     9001 gtgcagcctg cgccaggcta gatgaagcgt tggatcagca gcacgacaaa gaacagcacc
     9061 gcaccggcga tgccgatgcc aaacaaccgc ccggtccagc gcaagtactt acggtcgccg
     9121 ctgaaaaggc tcagcgcgac aagcacgatg atcgtcgccg caaagacgaa cagcaacgcg
     9181 cggaaatacg gcactgcgtt agcgtgctac gccggcaccg gctgccgtag gccggccggc
     9241 gccgccgccg catcctcgaa caccactagt tcatacgcgg ccttatccgc cagcagcgca
     9301 cgcaccagct ggttgttcag cgcgtgaccc gatttgtgcg cgacgtaagc cgcgatcagc
     9361 gggcgaccga gcacgtacag gtcgcccatc gcgtcgagaa tcttgtgctt ggcgaattcg
     9421 tcgcccgagc gcaggccatc ggtattgagc acgcgatact cgtccatcac gatcgcgttc
     9481 tggaagttgc cgccgagccc gaggccggcg gcacgcagcg cttcgacctc gttgacgaaa
     9541 ccgaacgtac gtgcgcgcgc gaccgcggtc acgtacgagt cgcgtgcgaa gtcgacctcg
     9601 acgtcctgat cggtcgcgtc gatcgccggg tgagcgaagt cgattgagaa gcgcagcttg
     9661 tagccgtcat aaggctccag ccgcgcccac ttctcgtcgg caccggcacc ggcccgcacc
     9721 tcgactcgcc tgcggacgcg gatgaagcgc ttcggcgcac tcaactcgac gatacccgcc
     9781 gactgcaaca gaaacacgaa gctcgcagcg ctgccgtcca tgatcggaac ttcggccgca
     9841 tcgacgtcga cgaaggcgtt gtcgataccg agcccggcaa gcgcgctcat caaatgctcg
     9901 atcgtcgcca ctcgagcgcc gttctgcacc agcgtcgacg ccatgcgcgt gtcgccgatc
     9961 gcctcgggac ggaccgggat gtcgaccacc ggatcgaggt cgatacgccg gaagactatg
    10021 ccagtgtccg ccgcggccgg ccgcagcacg agttcgacct tcgtgccgct gtgcatcccg
    10081 atgccgaccg tgcgcaccag tgtcttcagt gttcgttgtt tgagcatggt gtccgaattc
    10141 taagggtcgc gatcacgcac tcgacgatcg ccacgcgggc ggacacaatg ccggcaccct
    10201 cacgactgct ccgatgaacg agcccgcacc gctcatgccg tccgcctcgc cattcccggc
    10261 gctgcgcacg gtgtcgttcg gcgcgccgct gggctggctg tccagggccg cgactgacct
    10321 gcgcgccgcg cccagcgcga gccttttcta tggtgctgca tttgcctcca tgggctgggc
    10381 gatctacttc gtgttccgcc acgcatacga gtatgcgtcg gcactgacag cgggtttcct
    10441 gctcctcggt ccgtttctgt gcacggggct gtacgacatc agtcgccgcg tcgagcacgg
    10501 cggatctgcg cacctggcgc cgacactgac ggcatggcgc gccaaccttg gcgcgttctc
    10561 gctgtttgct ctgatcctga ccgtgctgat gctgatctgg gcgcgcgcgt cgctggtgac
    10621 gttcgcgctc ttcttctcga gcgggatgcc gacgctgagc ggtttcctcg agcaggtcgt
    10681 aagcgtcgag cactgggact tcgtgctgac gtggctcgca gtcggcgcca tcttcgccgc
    10741 catcgtgttc gcggtcagcg tggtctcggt gccgatgatg ctcgatcgcg gcaccgacac
    10801 cgtcgtcgcg gcgctgacca gcgtgcgcgc tctgctggcc aatcctggcc cgctgctggc
    10861 ctgggcggcg ctgatcgtgc tgttgatcgg cgtgggattc gcgacggctt tcatcggcct
    10921 cgtcatcacc gcgccgctgg tcgggcatgc aacgtggcac gcgtaccggg agattgtggt
    10981 ccgtgatcgg taagccgtga tccgtgggcc gcacggatca cggtcgacga accacggatc
    11041 gcgagcctag tcggcctgct tgcgcaggaa cgccgggatg tcgaagcggt cgtggcccgc
    11101 atcctccatt gcggacacgc gcgcctgcgc gctttcgcga gagcgccaca ccgcgggtgt
    11161 gtcgaagtgg ctgtaatcgc cgctcgaagc accgccattg gcgtgcgcga ccggcggcat
    11221 gacgaccgga gtgtcgtgcg tgccggtctt gatgacagtg aacggggtct gcttcttggc
    11281 ggtcgaagtc cgaccgagcc cggtcgcgac cacggtcacg cgcaggtttt cccccatcga
    11341 ctcgtcgcac acggtgccga agatcaccgt cgcatcctcg gcggcgaagg cgcggatcgt
    11401 gttcatcact tcgcgcgtct ccttcatccg cagcgagttg ctggcggtga tgttgaccag
    11461 cacgccacgc gcgcccgaca gatccacgcc ctccagcagc gggctggcga ccgcctgttc
    11521 ggcggcgatg cgcgcgcgat ccagccccgc ggcggtcgcg gtgcccatca tcgctttgcc
    11581 atgctcgctc atcacggtct tcacgtcctc gaagtcgacg ttgaccaggc cgggaatgtt
    11641 gatgatctcg gcgatgcccg cgcatgcgtt gtgcagcacg tcgtcggcgg cgcggaagca
    11701 ttcctcgaag ctcgcgtcct cgcccagcac ttcctcgagc ttgttgttca ggatcacgat
    11761 cagcgagtgc acgcgttcct ggagctcgcc cagcccgtcc tccgcgaccc tcattcgccg
    11821 cgggccctcg aactcgaacg gcttcgacac gaccgcaaca gtcaggatgc cgagctcttt
    11881 cgcgatttcg gcgaccacgg gcgccgcgcc ggtgccggtg ccgccgccca tgccggcggt
    11941 gatgaacacc atgtgcgcac cgcgcagcgc atcggcgatg cggtcgcggc cgtcgatcgc
    12001 ggactgccgt cccgcctccg gcctgctgcc cgcgcccagc ccggtcgtgc cgagctggat
    12061 gatgttcttg gccgccgagc gcgccagcgc ctggtgatcc gtattggcgc agatgaactc
    12121 cacgccctgt acgccgcgcc ggatcatgtg ctcgatcgcg ttgccgccgg cgccgccgac
    12181 gcccaccacc ttgatcaccg tgccccgtgt ttctgtttcg agaatctcaa acgccatgct
    12241 tgcctccaag gttgctcaat gcaccgatac cgaaacgcgc aatgtcaatg gcgacgcggg
    12301 ctcatgttct ttgtcgtctt tgacactgca cgcttcccct aaaaattgcc gacgatccat
    12361 tccttcatcc gtttcagcgt ctgcttgaac gagcccgact gcgccgcgac cttgcggccg
    12421 cgcacccgct gcagatgcgc ctccgtcaga agccccatca ccgtggcaaa acgcgggttg
    12481 cgaatcacgt cggcgagcgc gccgtcgtaa gttggccagc cgatccgcgc cggcttcaga
    12541 aacacgtctt ccgccagctc gcccacgccg ggcagcaggc tcgtgccgcc ggtgagcacg
    12601 acgccggacg acagcagttc ctcgtagcca gaatcgcgga ccacctgctg cgtcagcgtg
    12661 aagagctcct ccacgcgcgg ctcgatcacc gccgccagcg cctgccgcga caacagccgc
    12721 ggtgcgcgat cgccgatgcc gggaatctcg atgcggtcgt tcggatccgc cagcacctcc
    12781 ttcgcgacac ccgagcgcag cttgatctcc tccgcgtccg ggatcggcgt gcgcaatgcc
    12841 atcgcaatgt cgttggtgat ctggtcgccg gcgatcggga tcaccgcggt gtggcggatc
    12901 gcgcccccgg tgaagatcgc gatgtcgctg gtgccgccgc cgatgtcgac cagcgccacg
    12961 cccagttcct tctcatcggg cgtgagaacc gaaagactcg acgccagcgg ctgcaggatc
    13021 aggtcctgca cctcgagccc gcagcggc
//
LOCUS       DBNHBY010000774         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1074270, whole genome shotgun sequence.
ACCESSION   DBNHBY010000774 DBNHBY010000000
VERSION     DBNHBY010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1074270"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="AC16HB_18210"
     CDS             <1..647
                     /locus_tag="AC16HB_18210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5608780.1"
                     /translation="DQVVPGGVRRDLQPAQTELIRALLRDCRKRFPELIELYDNTASL
                     QDRTVGTGVLSAELVRQFGCGGYIGRASGRAFDARRTPGYPPYDDLHFEVPVLEDGDV
                     NGRIWIRVREVEQSIALIDQIIDRLPPGPIGTTAGTLSASGDREGMALVEGFRGDILV
                     WATLRPDGTIERCHLRDPSWFQWPLLEAAIEGNIVADFPLCNKSFNCSYSGHDL"
     gene            683..1225
                     /locus_tag="AC16HB_18215"
     CDS             683..1225
                     /locus_tag="AC16HB_18215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5608781.1"
                     /translation="MRRMLFKSMVHGPLTEPAPTVDDAALAELAAQVDRVARQRLGRT
                     LSIREVDAGSCNGCELEIHALNNVIYDLERFGLRFVASPRHADVLLVTGPVTWNMREA
                     LERTYNATPAPKWVIAVGDCARDGGVFRDSYACVGGIGAVLPVDVHVPGCPPTPVALL
                     RALLALLESASTGPGAQARA"
     gene            complement(1388..1885)
                     /locus_tag="AC16HB_18220"
     CDS             complement(1388..1885)
                     /locus_tag="AC16HB_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608782.1"
                     /translation="MRRWLPAFALTLALGACDQQGRPLDWPGLDKLKPGQSTELDVRQ
                     VLGLPDVVVDQPGGGKLLQFPKGPEGVSTFFAVLGPDGKLVSVKNVLTHDTFNQIKAG
                     MSRADALALLGRPAREHAYPLKQQVAMEWTINDSGEKKLFVVTLSADGKVVSSGVEPI
                     SRGGG"
     gene            1992..2183
                     /locus_tag="AC16HB_18225"
     CDS             1992..2183
                     /locus_tag="AC16HB_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HIR5608783.1"
                     /translation="MAAYESDITRFLRELKQSKPDLERKQREGRALLWDQQLDFDELA
                     RWQESKVPQQPYVYQTKTK"
     gene            2184..>2634
                     /locus_tag="AC16HB_18230"
     CDS             2184..>2634
                     /locus_tag="AC16HB_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5608784.1"
                     /translation="MSTEPSAPLLHRRLDEPQVDSTPDVVDGIALARLYGEPLFKLPD
                     DLYIPPDALEIFLEAFEGPLDLLLYLIRKQNFNVLDIPMAPLTQQYLAYVEQIRRSNL
                     ELAAEYLLMAAMLIEIKSRMLLPVKKAETGEEVEDPRAELVRRLIEYE"
BASE COUNT          427 a          907 c          863 g          437 t
ORIGIN      
        1 tggatcaggt cgtgcccggc ggcgtacgcc gggatctgca gccggcgcag acggaactga
       61 tccgcgcgct cctgcgcgat tgtcgcaagc gctttcccga gctgatcgag ctgtacgaca
      121 acaccgcgtc gctgcaggat cgcacggtcg gaaccggagt cttgtccgcc gaactcgtac
      181 ggcagttcgg ctgcggcggc tacatcggcc gcgccagcgg ccgcgcgttc gacgcgcgac
      241 gcacgccggg gtatccaccg tatgacgacc ttcatttcga ggtcccggtc ctggaagacg
      301 gcgacgtgaa cgggcgcatc tggattcgcg ttcgcgaggt cgagcagagc atcgcattga
      361 tcgaccagat catcgaccgg cttccgcccg ggccgatcgg cacgacagcg ggcacgctgt
      421 cggcatccgg tgatcgggaa ggcatggcgc tggtcgaagg ctttcgcggc gacattctgg
      481 tctgggcgac gctgcgcccg gacggaacga tcgagcgctg ccatctgcgc gatccctcgt
      541 ggttccagtg gccactgctc gaagccgcga tcgagggcaa catcgtggcc gacttcccct
      601 tgtgcaacaa atcgttcaac tgttcgtatt ccgggcacga tctctagaac ccgtctcgga
      661 cgagacttcc aggtagtcgc cgatgcgcag gatgttgttc aagagcatgg tccacgggcc
      721 gttgaccgag cccgcgccga ccgtggacga tgccgcgctc gccgaactcg ccgcccaggt
      781 cgaccgcgtt gcgcgccagc gtcttggtcg aacgctgtcg atccgcgaag tcgacgccgg
      841 ctcgtgcaac gggtgcgaac tcgagatcca cgcgctcaac aacgtgatct acgatctcga
      901 gcgcttcggc ctgcgctttg tcgcctcgcc gcgccacgcc gacgtgctgc tcgtgacggg
      961 gccggtcacg tggaacatgc gcgaagcgct cgagcgcacc tacaacgcga cgccggcacc
     1021 gaaatgggtg atcgcggtcg gcgattgcgc gcgcgatggc ggcgtcttcc gcgacagcta
     1081 tgcgtgcgtg ggcggcatcg gcgcggtgct tccggtcgat gtgcacgtcc ccggctgtcc
     1141 gccgacgccg gttgcgttgc tccgagcact gctcgcgctt ctcgaatccg cgtcgacggg
     1201 gccgggcgcg caggcgcgcg cctgatcggg ccgcattgtc tcgggggcgg ttcggaagcg
     1261 cgtggcgaac gagtttccgg tcgacgtggc gttccccggc tgccaccacc gccgcgcgag
     1321 atcctgcgcg gcatcctgac tgccggcgcg acgcgcgcca gcggcacacg tcgcgcgcag
     1381 ccccgcgtca gccgccgccg cgcgagatcg gttccactcc gctcgagacg accttgccat
     1441 cggcgctcag cgtcacgacg aagagcttct tttcgccgct gtcgttgatc gtccattcca
     1501 tcgcgacctg ctgcttgagc ggatacgcat gctcgcgcgc cgggcgtccc agcagcgcca
     1561 gcgcgtcggc gcggctcatg ccggccttga tctggttgaa cgtgtcgtgg gtgagcacgt
     1621 tcttcacgct gaccagtttg ccgtccggcc ccagcacggc gaagaacgtc gatacgccct
     1681 cgggaccctt cggaaactgc agcagcttgc cgccgcccgg ctgatcgacg acgacgtccg
     1741 gcaggcccag cacctggcgc acgtcgagct cggtcgactg accgggcttc agcttgtcga
     1801 gtccgggcca gtccagcggc cgcccctgtt gatcgcacgc gcccagcgcc agcgtcagcg
     1861 cgaacgcggg taaccatctt ctcatcgccg tcctccctgt cggaactccg gtcacgttag
     1921 caggccaggc gccatggcgc gtcccgactt cgcgcggtcg tgcgcgatcg cacccgtaca
     1981 atccgatccc catggccgca tacgaatccg acatcacgcg cttcctgcgc gagctcaagc
     2041 agagcaaacc cgatctggag cgcaagcagc gcgaaggacg cgcgctgctg tgggaccagc
     2101 agctcgactt cgacgagctt gcgcgctggc aggaatcgaa ggtgccgcag cagccgtacg
     2161 tgtaccagac gaagacgaag tagatgtcga ccgaacccag cgcgccgctg ctgcaccggc
     2221 ggctcgacga gccgcaggtc gacagcacgc ccgacgtggt cgacggcatc gcgctggcgc
     2281 gcctgtacgg cgagccgctg ttcaagctgc ccgacgatct gtacatcccg ccggatgcgc
     2341 tcgagatctt cctcgaggcg ttcgaaggcc cgctcgacct gctgctgtac ctgattcgca
     2401 agcagaactt caatgtgctc gacatcccga tggcgccgct gacgcagcag tacctcgcgt
     2461 acgtcgaaca gatccgccgc agcaacctgg agctggcggc cgagtatctg ctgatggcgg
     2521 cgatgctgat cgagatcaag agccggatgc tgctgccagt gaagaaggcc gagaccggcg
     2581 aggaagtcga ggacccgcgc gccgaactcg tgcgccgact gatcgagtac gagc
//
LOCUS       DBNHBY010000775         6977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1008075, whole genome shotgun sequence.
ACCESSION   DBNHBY010000775 DBNHBY010000000
VERSION     DBNHBY010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1008075"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="AC16HB_18235"
     CDS             <1..783
                     /locus_tag="AC16HB_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608785.1"
                     /translation="RAQDLLQKNFVSESSVDTALARYNKARAATESLKAQIAVAEANL
                     RVATVALDQTLIRAPFAGIVLTKSANVGDIVTPFSSASGTTGAVVTMADMSTLEVEAD
                     VSESSIAKIAVGQPAEIQLDAFAELRLLGSVSRIVPTVDRSKATLLVKVRFDETDPRV
                     LPDMSAKIAFLSRKPEPAERKPVAALRPEAIVRRNDRDIVFVVKGDSDKSEGGTAAMT
                     PIAAGPKVGDLVRVDALAAGTRVVINPPAKLQDGQTVAAAKK"
     gene            784..1506
                     /locus_tag="AC16HB_18240"
     CDS             784..1506
                     /locus_tag="AC16HB_18240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608786.1"
                     /translation="MGALRTTGSGREALVAIDRVSKSYVRGEQVVPVLTDINLEIGRG
                     DFVALMGPSGSGKSTLLNLVAGIDKPDAGRIVIDGEDITRLDEGDLAAWRARAVGFIF
                     QFYNLMPVLTAFENVELPLLLTALTRRERRERVETVLGLVGLADRMDHRPNELSGGQQ
                     QRVAIARAVVTDPLLLIADEPTGDLDRTSAAEILKLLDRLNIELGKTIVMVTHDPRAA
                     EAAHHLIYLDKGQLTDTSPFVH"
     gene            1510..2670
                     /locus_tag="AC16HB_18245"
     CDS             1510..2670
                     /locus_tag="AC16HB_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608787.1"
                     /translation="MFFLRLVLRNVFRARLRAVLTVLGLVIALVAFGLLQTVVTAWYA
                     GSEAASASRLVTRNSISLVFPLPMYYREKILAIGGVRGVAVSNWFGGIWRDEKSFFAQ
                     FATDVPNFFAIYPEFRFKPEEWQNFLRDRQGAAVGRQLADLYGFKVGDRVPIRGTIYP
                     GNWEFTVRAIYDGRDETTITRQFYFHYDYLNEFLKQRFPRAANNAGVFVVDVDDPARG
                     AEVARTIDAEFANSLAATLTETEKAFQLSFVAMVDTIIQVISGVSFIVIAIIFAVAAN
                     TMAMTARERLSEYATLKALGFGPAFVARLILAESVALALLGGAIAIAVTPGIAQGFWN
                     AVGKTVFARFEVAPTTLAQQAIAALAVGAFAAVVPMVRSARVKIVDGLRHVG"
     gene            2671..3837
                     /locus_tag="AC16HB_18250"
     CDS             2671..3837
                     /locus_tag="AC16HB_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608788.1"
                     /translation="MRIPLSYIARNLWVRRLTTVLTAGGMALVVFVFAAVLMLDAGLK
                     ATLVATGADDNVLFIRKGAETEIQSGVARDQAALIESLPQIGRDERGAPRVSKEVVVL
                     NSLPKLDTGKRTNVPIRGVTPMGLELRPHVKIVQGRMFRFGSSEIVVGASVERQFAGV
                     QLGQALSFAQRQWTVVGVFDAGRTAFDSEIWGDAEQLAQAFRRTGFSSVIAQLASHDA
                     YDSLQQALAGEPRLTAEVKRERQFYEDQSRALSGFISYLGLTLSIIFSLGAIIGATIT
                     MYASVATRTGEIGTLRALGFRRRSILAAFLLESLLLALAGGLVGLSAATFLQTITIST
                     TNFTSFSELAFSFALTPKIVADSLVFALAMGMVGGFLPAVKASRMKIVDALRAD"
     gene            3852..5093
                     /locus_tag="AC16HB_18255"
     CDS             3852..5093
                     /locus_tag="AC16HB_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5608789.1"
                     /translation="MSDPLPLNGVRVVEFTHMVMGPTCGLILADLGAEVIKVEPVTGD
                     NTRRLLGSGAGFFALFNRNKKSLAVDARDARGREIVLKLVATAEVFSENFKGGTMAAL
                     GLDYASLAKLNPRLVYVSHKGFLPGPYEHRTALDEVVQMMGGLAYMTGPEGRPLRAGS
                     SVNDIMGGMFGAIGALAALRERERTGRGQEVGAALFENTVFLVAQHMMQFAVTGQPAA
                     PMPSRISAWGIYDVFSVADGAQIFLAVVSDTQWPLFCDAFGFADLRGDARLVTNNDRV
                     RARDWLIPLLRERLASHRAEEIAAVFESRGLPYAPITRPQDLIDDPHLAATGGLAQTA
                     IPADGSSAGHALQARAPLLPLTLGGCRLPLRSNPPALGEHTDALLAELGYRAEEIDQL
                     HADGVIGAVRGSPPLREPADA"
     gene            5147..6109
                     /locus_tag="AC16HB_18260"
     CDS             5147..6109
                     /locus_tag="AC16HB_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5608790.1"
                     /translation="MKRRDFSLATVAALTAAQFPLAARAQAIGTLKMMIPANPGGGWD
                     QTGRALGSAMQAAKVVQSVQFDNKGGAAGTIGLTQFINAGKGDPNALMVGGLVMVGGI
                     VLNKSPVDLTMVTPIARLTSEYEVIVVPAASPHKTMGDLLKAFKADPGKVAWNGGSAG
                     GTDHMLAGLIARAVGVDPTKVNYVASKGGGDQIANIVGGHVTAGIAGLGEFAEHIKGG
                     RMRALAVSSPSKQEGIASLKEQGVDVVLGNWRGVFGAPAITTAQRDALINAVKTAVES
                     PAWQETLKKMDWQSVFLAGDAYKTFIDEETKRAKGILESLGLAK"
     gene            6113..6625
                     /locus_tag="AC16HB_18265"
     CDS             6113..6625
                     /locus_tag="AC16HB_18265"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5608791.1"
                     /translation="MWPATRGARVDLILSLGVLALGVFASIIAFRLPEAGGYARIGPN
                     FMPKVVSGGLIVLGLWLLAEVFTGGWRERVPDDPAERGDHAFHAGAFAWVSAGLFAQM
                     ALIGNAGFVIAAMALFGCVARGFGSARAVRDLVIGLALGLAVFLFFVKFLNVNLPAGW
                     LMPLLGTAGI"
     gene            6622..>6977
                     /locus_tag="AC16HB_18270"
     CDS             6622..>6977
                     /locus_tag="AC16HB_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5608792.1"
                     /translation="MSETLSALLHGFGVALEPLNLLWGFVGVTLGTAIGVLPGVGPAL
                     TVAMLLPLTVKLDPTGALIMFADIYYGAMYGGSTTTILLNTPGESASIATALEGNKMA
                     KRGRAGPALATAAIGS"
BASE COUNT         1073 a         2272 c         2502 g         1130 t
ORIGIN      
        1 cgcgcgcagg atctgttgca gaagaatttc gtgtccgaat cgtcggtcga cacggcgctc
       61 gcgcgctaca acaaggcgcg cgcggcgacc gagtcgctga aggcgcagat cgcggtcgcc
      121 gaggccaacc tgcgcgtggc tacggtggcg ctcgaccaga cgctgatccg cgcgccgttc
      181 gccggcatcg tgctgaccaa gagcgcgaat gtgggcgaca tcgtcacgcc gttctcgtcg
      241 gcctccggca cgaccggcgc ggtcgtcacg atggccgaca tgagcacgct cgaagtcgag
      301 gccgacgtgt cggagtcgag catcgcgaag atcgcggtcg gccagccggc cgagatccag
      361 ctcgatgcgt ttgccgagct gcggctgctg ggctcggtat cgcgcatcgt gccgacggtg
      421 gatcgcagca aggccacgct gctggtcaag gtgcgcttcg acgagaccga tccgcgcgtg
      481 ctgccggaca tgagcgcgaa gatcgcgttc ctgtcgcgca agcccgagcc cgccgagcgc
      541 aagccggtgg cagcgttgcg ccccgaggcg atcgtccggc gcaacgaccg ggacatcgtg
      601 ttcgtggtca agggcgacag cgacaagagc gagggcggca ccgcggccat gaccccgatc
      661 gcggccggcc cgaaggtcgg cgacctcgtg cgcgtcgatg cgctcgcggc ggggacgcgc
      721 gtcgtgatca atccgcccgc caagctgcag gacgggcaga ccgtcgccgc ggcgaagaag
      781 tagatgggtg cgttgcgcac caccggcagc ggacgcgagg cgctggtcgc gatcgaccgc
      841 gtcagcaaga gctacgtgcg cggcgagcag gtggtgccgg tgctgaccga catcaacctc
      901 gagatcggcc gcggtgactt cgtcgcgctg atggggccga gcggatcggg caagagcacg
      961 ctgctgaacc tcgtcgcggg gatcgacaag cccgatgccg gtcgcatcgt gatcgacggc
     1021 gaggacatca cgcggctcga cgagggcgac ctcgccgcgt ggcgcgcccg cgcggtcggc
     1081 ttcatctttc agttctacaa cctgatgccg gtgctgaccg cgttcgagaa cgtcgaactg
     1141 ccgctgctgc tcacggcgct cacgcggcgc gagcggcgcg aacgcgtcga gacggtgctg
     1201 gggctggtcg ggctggccga tcgcatggac catcggccca acgagctgtc gggcgggcag
     1261 cagcagcgcg tggcgatcgc gcgcgcggtc gtcactgatc cgctgctgtt gattgccgac
     1321 gagccgaccg gcgacctcga tcgcacgtcg gccgcggaga tcctgaaact gctcgaccgc
     1381 ctgaacatcg aactcggcaa gacgatcgtg atggtcacgc acgatccgcg cgctgccgag
     1441 gccgcgcatc acctgatcta tctcgacaag ggacaactga cggacacgag tccgttcgtg
     1501 cattgagccg tgttcttcct gagactcgtt ctgcgcaacg tctttcgcgc ccggctgcgt
     1561 gcggtgctga cggtgctcgg actggtgatc gcactggtcg cgttcggcct gctgcagacg
     1621 gtcgtcacgg cctggtacgc gggctcggag gccgcgtcgg cgtcgcggct ggtcacgcgc
     1681 aacagcattt cgctggtgtt tccgctgccg atgtactacc gcgagaagat cctggcgatc
     1741 ggcggcgtgc gcggcgtggc ggtatcgaac tggttcggcg gcatctggcg cgacgagaag
     1801 agtttcttcg cgcagttcgc caccgacgtg ccgaatttct tcgcgattta ccccgagttc
     1861 aggttcaagc cggaggagtg gcagaacttc ctgcgtgacc gccagggcgc ggcagtcggc
     1921 cggcaactgg cggacctgta cggcttcaag gtcggcgacc gcgtgccgat tcgcggcacc
     1981 atctatccgg gcaactggga gttcaccgtg cgcgcgatct acgacggccg cgacgaaacg
     2041 acgatcacgc gccagttcta cttccactac gactacctga acgagttcct caagcagcgc
     2101 ttcccgcgtg ccgccaacaa tgccggcgtg ttcgtggtcg acgtcgacga tccggcccgc
     2161 ggcgcggagg tcgcgcgcac gatcgatgcc gaattcgcca attcgctcgc cgccacgctg
     2221 accgagaccg aaaaggcatt ccagctttcg ttcgtcgcga tggtcgacac gatcatccag
     2281 gtgatctcgg gcgtgagctt catcgtgatc gcgatcatct tcgcggtggc ggccaacacg
     2341 atggcgatga ccgcgcgcga gcgcttgagc gagtacgcga cgctgaaggc gctcggcttc
     2401 ggtccggcct tcgtcgcgcg gctgatcctg gccgaaagcg tcgccttggc gctgctcggc
     2461 ggcgcgatcg cgatcgcggt gacgcccggc atcgcgcagg gattctggaa tgcggtcggg
     2521 aagaccgtgt tcgcgcgctt cgaggtcgcg ccgaccacgc tcgcgcagca ggccattgcg
     2581 gcgctcgcgg tcggcgcatt tgccgcggtg gtgccgatgg tgcgcagcgc ccgcgtgaag
     2641 atcgtcgacg gattgcggca cgtgggctga atgcgcatac ccctctcgta catcgcgcgc
     2701 aacctgtggg tgcggcggct gacgaccgtg ctgaccgccg gcggcatggc gctggtcgtg
     2761 ttcgtgttcg ccgccgtgct gatgctcgac gccgggctca aggcgacgct ggtggcaacc
     2821 ggggcggacg acaacgtgct cttcatccgc aagggcgcgg agacggagat ccagagcggt
     2881 gtggcgcgcg accaggcggc gctcatcgag agcctgccgc agatcgggcg cgacgaacgc
     2941 ggggctccgc gcgtgtcgaa ggaagtggtg gtcctcaatt cgctgccgaa gctcgacacc
     3001 ggcaagcgca ccaacgtgcc gatccgcggc gtgacgccga tgggcctcga gctgcggccg
     3061 cacgtgaaga tcgtccaggg acggatgttc cgcttcggct cgagcgagat cgtggtgggt
     3121 gcgagcgtgg agcgccagtt cgcgggtgtg cagctcgggc aagccttgtc gttcgcgcag
     3181 cggcagtgga ccgtggtcgg cgtgttcgac gccggccgca ccgcgttcga ctcggagatc
     3241 tggggcgatg cggagcaact cgcgcaggcg ttccgccgca ccggcttctc gtcggtgatc
     3301 gcccagctgg cgtcgcacga cgcgtacgac tcgctgcagc aggcgctcgc cggcgagccg
     3361 cggctcaccg ccgaggtcaa gcgcgagcgg cagttctacg aggaccagtc gcgtgcgctg
     3421 tcgggcttca tcagctacct ggggctcacg ctgtcgatca tcttctcgct gggggcgatc
     3481 atcggcgcga cgatcacgat gtacgcgtcg gtcgccacgc gcaccggcga aatcggcacg
     3541 ctgcgcgcgc tcggcttccg gcgccgttcg atcctggccg cgttcctgct cgaatcgctg
     3601 ctgctcgcgc tcgccggcgg gctggtcggg ttgtctgcgg cgacattcct gcagacgatc
     3661 acgatctcga cgaccaactt cacgtcgttc tccgaactcg cgttctcgtt cgcgctgacg
     3721 ccgaagatcg tcgccgacag cctggtgttc gcgctcgcga tgggcatggt cggcggcttc
     3781 ctgccggccg tgaaggcgag ccggatgaag atcgtggatg cgctgcgcgc ggattgaatg
     3841 cgagagggcc catgtccgat cctctgccac tgaacggcgt gcgagtcgtc gagttcacgc
     3901 acatggtgat gggcccgacc tgcgggctga tcctcgccga tctgggcgcg gaggtgatca
     3961 aggtcgagcc ggtgaccggc gacaacacgc gccggctgct cggttcgggc gccggattct
     4021 tcgcgctgtt caaccgcaac aagaagagcc tcgcggtcga cgcgcgcgat gcgcgcgggc
     4081 gcgagatcgt gctgaagctt gtggccaccg ccgaagtgtt cagcgagaac ttcaaggggg
     4141 gaacgatggc ggcgctcggc ctcgactacg cgtcgctggc gaaactgaat ccgcggctcg
     4201 tgtacgtgtc gcacaagggc ttcctgccgg gaccgtacga acaccgcacc gcgctcgacg
     4261 aagtggtgca gatgatgggc ggcctggcct acatgaccgg gccggagggt cggccgctgc
     4321 gcgcaggttc atcggtcaac gacatcatgg gtgggatgtt cggcgcgatc ggcgcgctgg
     4381 cggcgctgcg cgagcgcgag cgcaccgggc gcgggcagga ggtgggcgcc gcgctgttcg
     4441 agaacacggt gttcctggtc gcgcaacaca tgatgcagtt cgcggtcacg ggccaacccg
     4501 ctgcgcccat gccctcgcgc atctcggcgt ggggaatcta cgacgtgttc tcggtggcgg
     4561 acggcgcgca gatcttcctc gcggtggtgt cggacacgca gtggccgctg ttctgcgacg
     4621 cgttcggctt cgccgacctg cgaggcgacg cacgactggt gaccaacaac gaccgcgtgc
     4681 gcgcgcgcga ctggctgatc ccgctgctgc gcgagcggct cgcgagccac cgcgcggaag
     4741 agatcgcagc ggtcttcgag tcgcgcgggc tgccgtacgc gccgatcacg cggccgcagg
     4801 acctcatcga cgatccgcac cttgcggcga ccggcggcct cgctcagacc gcgattcccg
     4861 ccgacggcag cagcgcgggg catgcgctgc aagcgcgcgc gccgctgctg ccgctgacgc
     4921 ttggaggatg ccgcctgccg ctgcgatcga acccgccggc gctgggcgag cacaccgatg
     4981 cgctgctcgc cgaactcggc taccgcgctg aggagatcga ccagctccat gcggacggcg
     5041 tgatcggcgc agtgcgcggc agcccgccgc tacgcgaacc cgccgatgcc tgatcgctgg
     5101 tgcgctgcta ccatcgccgc gccaggaact cgcaaaggag acagggatga aacgccgaga
     5161 tttctcgctg gccacggtcg ctgcgctgac agcggcgcag ttcccgctcg ccgcgcgcgc
     5221 gcaggcaatc ggcacgctga agatgatgat tcccgccaac cccggcggcg gctgggacca
     5281 gaccggacgc gcgttgggca gcgcgatgca ggcggcgaag gtcgtgcagt cggtgcaatt
     5341 cgacaacaag ggcggagcgg caggcacgat cgggctgacg cagttcatca acgccggcaa
     5401 gggtgacccg aacgcgctga tggtcggcgg cctcgtgatg gtcggcggca tcgtgctgaa
     5461 caagtcgccg gtggacctga cgatggtgac gccgatcgcg cggctgacca gcgagtacga
     5521 ggtgatcgtg gtgccggcgg cgtcgcccca caagaccatg ggagatctcc tcaaggcatt
     5581 caaggccgat ccgggcaagg tcgcatggaa cggcggttcg gcgggcggca ccgaccacat
     5641 gctggccggg ctgatcgcgc gcgcggtcgg ggtcgacccg accaaggtca actacgtggc
     5701 atccaagggc ggcggcgacc agatcgccaa catcgtcggc gggcatgtga cggccggtat
     5761 cgcagggttg ggcgaattcg ccgagcacat caagggcggc cgcatgcgcg cgctcgcggt
     5821 gtcgtcgccg agcaagcagg agggcattgc ttcgctgaag gagcaaggcg tcgacgtcgt
     5881 gctcggcaac tggcgcggcg tgttcggcgc gccggcgatc accaccgcgc agcgcgacgc
     5941 gctgatcaac gcggtgaaga cggcggtcga gtcgccggcg tggcaggaga cgctcaagaa
     6001 gatggattgg caatcggtgt ttctcgccgg ggacgcgtac aagacgttca tcgacgagga
     6061 aacgaagcgc gccaagggca tcctcgaatc gctggggctc gcgaagtagc cgatgtggcc
     6121 cgcaacgcgc ggcgcgcgcg ttgacctgat cctgtcgctc ggggtactgg cgctaggcgt
     6181 cttcgcgtcg atcattgcat tccgcctgcc ggaggcgggc ggctatgcac gcatcggccc
     6241 gaacttcatg ccgaaggtcg tttccggcgg gctcatcgtg ctgggcctgt ggctgctcgc
     6301 cgaggtcttc accggcggct ggcgcgaacg cgtgcccgac gacccggccg agcgcggcga
     6361 ccacgcattc cacgccggag cgttcgcctg ggtcagcgct ggcctgttcg cacagatggc
     6421 gctgatcggc aacgccgggt tcgtgatcgc ggcgatggcg ctgttcggtt gcgtggcgcg
     6481 cggcttcggc agcgcgcgcg cggtgcgcga cctggtcatt ggactggcgc tcgggctggc
     6541 ggtgttcctg ttcttcgtga agttcctgaa cgtcaatctg ccggcggggt ggctgatgcc
     6601 gctgctcggc accgccggca tatgagcgag accctgtccg cgctgctgca cggcttcggt
     6661 gtcgcgctgg agccgctgaa cctgctgtgg ggcttcgtcg gcgtcacgct cggcaccgcg
     6721 atcggcgttc tgcccggcgt cggtcccgcg ctgacggtgg cgatgctgct gccgctgacg
     6781 gtcaagctcg atccgaccgg cgcgctgatc atgttcgccg acatctacta cggcgccatg
     6841 tacggtggct cgaccaccac gatcctgctg aacacgccgg gcgaatcggc gtcgatcgcg
     6901 accgcgctgg aaggcaacaa gatggccaag cggggtcggg cagggcccgc actcgcaacc
     6961 gcggccatcg gctcgtt
//
LOCUS       DBNHBY010000776         4672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1008397, whole genome shotgun sequence.
ACCESSION   DBNHBY010000776 DBNHBY010000000
VERSION     DBNHBY010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1008397"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC16HB_18275"
     CDS             <1..494
                     /locus_tag="AC16HB_18275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5608793.1"
                     /translation="HGRGELVIARSRSSEGIVRLFGTAGVPVQVSDNVIGALWGKLIV
                     NCAYNALSAITQMPYGRLVQGDGVPDVMRDVVGECLAVAQRAGVTVPGDPWEAVQRIA
                     QTMATQQSSTAQDLARGRPTEIDHLNGYVVRKGEALGVATPVNRALHALVRLLESRDR
                     SPR"
     gene            complement(646..1161)
                     /locus_tag="AC16HB_18280"
     CDS             complement(646..1161)
                     /locus_tag="AC16HB_18280"
                     /inference="COORDINATES: protein motif:HMM:NF018024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR5608794.1"
                     /translation="MTWSIIARDPATGAFGIAVATRFFAVGALCPHGDGGIGALASQA
                     LINPTYGPRGLRLLRGGLTAQSVVDALTLADAGRAARQLHVQDAAGRIAAHTGAECVP
                     WCGHAIHDTYSVAGNMLAGPQVLGETARAYDTNAGLPFPRRLIAALKAGEAAGGDKRG
                     RQSAALTIFTT"
     gene            complement(1158..2582)
                     /locus_tag="AC16HB_18285"
     CDS             complement(1158..2582)
                     /locus_tag="AC16HB_18285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR5608795.1"
                     /translation="MKPLSGLDGAFLHLETPETPMHVGSLHLFDLPPRFKGDFHAAIK
                     RQMRQRLHLAPVFTRKLANMPLQFANPVWVTDERVDLDYHVQRVTLPPPGTQAQLEDE
                     AARLHSELLDRSRPLWRLYVFAGLQSGQAAYYIKVHHAVLDGQAGVLLAQALFDLTPK
                     PPPLRTAAKHAAVEHPGRAELAAAALKHDAGQFIKLVRHLPDVMRTLAGMFRPAAGAA
                     KGQLGQNFAFGPKTPINVAIGGERGFAALSLPLDRLKDVAAAHDAKLNDVVLALCSGA
                     LRRYLSRHGGIPKKPLIAAMPISLRAPGDTEYTTQATMSLVNLNTHIADPVKRLRAIR
                     DAAGAVKALAKRARSVLPTDFPSIATPWVLHGLASLYGRSHLADAIPPIANVIISNVP
                     GPQVPLYAAGARMATYWPLSIPEHGVGLNITVMSYAGAMGFGFTAARNAVPDAREFAA
                     ALQAAFDELVGKTKSRARRASRAS"
     gene            2714..3526
                     /locus_tag="AC16HB_18290"
     CDS             2714..3526
                     /locus_tag="AC16HB_18290"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR5608796.1"
                     /translation="MVKRHPHEGEPHWFYTVTEWRALLEMATLPYSLPALATAPHGDG
                     HPVLLLPGFMADEYTLIALKLFLRQRGYDVHTWGFGRNVGFQRRHAAALEQKIRYMHH
                     KSGRKVSLLGWSLGGLFALYAAHQAPECVRGVITLGSPVSIDMDGSQSPPLVRALYQL
                     IAHPMGTSAHAMLPRTKQLRERRKPPVPTSCLYSLSDGVVPPQEATIEGDRRMHENVR
                     VPGSHVGLGFNAIVLYIVADRLAQPEGQWRPFRPQGVSGKIYRLMVREPAAA"
     gene            complement(3462..>4672)
                     /locus_tag="AC16HB_18295"
     CDS             complement(3462..>4672)
                     /locus_tag="AC16HB_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5608797.1"
                     /translation="PKPGDWRQFCIQASRIHARPLDLNRPLWEIYVIEGLDAFLDLPQ
                     GSFALLTKTHHAAIDVEQEAEITNLLHDITTTPPPPEPPEPWFAEPPPGTIAMVCRGV
                     ASTIMSPLRLAAPLTRAIRRVTPAALSLAGDILMRRERLPVTRFNSFVSPHRVFETRR
                     FTLDEFKTIRALVKGATVNDVVLAVCGGALRRYLEAEEELPRASLTAIAPIYIRDADA
                     APGSRPEVSWLHVKLGTDLADPVERLRAIHDHTRASDTVARAIGARELTDIGRHTPAS
                     TLAVTSKLLGRVALGMGRRAPLANCTITNVPGSLVPLYLCGARMTYFSAIMPIADGMG
                     LVFAVTSYDGRIVVSPTSCRELVPNPEAFAQCIRDSFQEYLAAAKKAVRPPRARAPSA
                     GRSSPRRLAA"
BASE COUNT          714 a         1589 c         1669 g          700 t
ORIGIN      
        1 accacggccg cggcgaactc gtcatcgccc gctcgcgatc gagcgaaggc atcgtgcggc
       61 tcttcggcac ggccggcgtg cccgtgcagg tgtcggacaa cgtgatcggt gcgctgtggg
      121 gcaagctcat cgtcaactgc gcgtacaacg cgctgtcggc gatcacgcag atgccgtacg
      181 gccggctggt acagggtgac ggcgtaccgg acgtgatgcg cgacgtggtg ggggagtgcc
      241 tcgcggtcgc gcagcgcgcg ggcgtcaccg tccccggcga tccatgggag gcggtgcagc
      301 ggatcgcgca gacgatggcg acccagcagt cgtcgaccgc gcaggacctg gcgcgcggcc
      361 ggcctaccga gatcgatcac ctcaacggct acgtcgtgcg caagggcgaa gccctcggcg
      421 tcgccacgcc ggtgaatcga gcgctgcacg cactggtcag gctgctcgag tcgcgcgatc
      481 gatcgccgcg ttgatgaaat cgcggtccca cacgccgggc gaggagcggg tggcgaagaa
      541 ctgcgcgaag tgcacaaagc gctcacggct caccgcttcg agccgctcca attccgaaag
      601 cggatcggtg tgatcgtcga cgcgcaggtc gagcgccggg tactcctagg tggtgaagat
      661 cgtgagcgcc gccgactgcc tgccgcgctt gtcgccaccc gcggcctcgc ccgccttcaa
      721 cgcggcgatc agccggcgcg ggaagggcag tccggcattg gtgtcgtagg cgcgggcggt
      781 ctcgccgagc acctgcggcc cggcgagcat gttgccggcg accgaatagg tgtcgtggat
      841 cgcgtgtccg caccacggca cgcactcggc gcccgtgtgc gcagcaatgc gcccggcggc
      901 gtcctgcacg tgcagttggc gtgcggcgcg gccggcatcg gcgagcgtca gcgcgtcgac
      961 caccgactgc gcggtgagtc ccccgcgcag cagtcgcagg ccacgcgggc cgtaggtcgg
     1021 gttgatcagc gcctgcgagg cgagcgcgcc gatgccgccg tcgccgtgcg ggcacagcgc
     1081 gccgaccgca aagaagcgcg tggcgactgc gatgccgaag gcgccggtgg cgggatcgcg
     1141 cgcgatgatc gaccaggtca tgacgctcgg ctcgcgcgtc gtgcccgcga cttcgtcttg
     1201 ccgaccagtt cgtcgaacgc cgcctgcagc gccgctgcga actcgcgcgc gtccggcacc
     1261 gcattgcgcg cggcggtgaa gccgaatccc atcgcgccgg cgtagctcat cacggtgatg
     1321 ttgagcccga cgccgtgctc ggggatcgac agcggccaat aggtcgccat ccgcgcgccc
     1381 gccgcgtaca gcggtacctg cggacccggc acgttcgaga tgatcacgtt ggcgatcggc
     1441 gggatcgcgt ccgcgaggtg cgagcgcccg tacagcgacg ccagcccgtg cagcacccac
     1501 ggggtggcga tcgacgggaa gtccgtcggc agcacgctgc gggcacgctt ggcgagcgcc
     1561 tttaccgcgc cggccgcgtc gcggatcgcg cgcaggcgct tcaccggatc ggcgatgtgc
     1621 gtgttcagat tcaccaggct catcgtcgcc tgcgtcgtgt actcggtgtc gccgggcgcg
     1681 cgcagcgaga tcggcatcgc ggcgatcagc ggcttcttcg gaatgccgcc atggcgcgaa
     1741 aggtatcggc gcaaggcgcc gctgcacaac gcgagcacca cgtcgttcag cttcgcgtcg
     1801 tgcgcggcgg cgacgtcctt cagcctgtcg agcggcagcg acagcgcggc aaagccacgc
     1861 tcgccgccga tcgcgacgtt gatcggcgtc ttcggcccga acgcgaagtt ctggccgagc
     1921 tgccccttcg ccgcaccggc cgcgggccgg aacatgcccg ccagcgtgcg catcacgtcg
     1981 ggcaggtggc ggaccagttt gatgaactgt ccggcgtcgt gcttcagcgc cgcggcggcg
     2041 agttcggccc tgcccggatg ctcgaccgct gcatgcttgg cagcggtgcg caacggcggc
     2101 ggctttggcg tgaggtcgaa cagcgcctgc gcgagcagca cgccggcctg gccgtcgagc
     2161 accgcgtgat ggaccttgat gtagtacgca gcctggccgc tctgcagacc cgcgaaaacg
     2221 tacagtcgcc acagcgggcg gctgcggtcg agcagttccg agtgcaagcg cgccgcttcg
     2281 tcctcgagct gcgcctgcgt gcccgggggc ggcagcgtca cgcgctgcac gtgatagtcg
     2341 agatcgacgc gctcgtcggt gacccacacc gggttggcga actgcagcgg catgttcgcg
     2401 agcttgcgcg tgaacacggg ggccaggtgc agccgctggc gcatctggcg cttgatcgcc
     2461 gcatggaagt cgcccttgaa gcgcggcggc aggtcgaaca ggtgcagcga ccccacgtgc
     2521 atcggcgttt cgggcgtctc caggtgcagg aacgcaccgt ccaggccact caggggtttc
     2581 atgacgtgac tctcgccgcg cgcgattgga aggatcgcac cattgtgctc cgcgtttgat
     2641 atgttcggct ctgcaaaccg cgcccggcac gacacaattc gcggcgaata cgaactcgat
     2701 caaatccgca cgcatggtca agcgacatcc gcacgagggc gaaccgcact ggttctacac
     2761 ggttaccgag tggcgcgcgc tgctcgagat ggccacgctg ccgtattcgc tgccggcact
     2821 ggcgaccgcc ccgcacggtg acggacatcc agtgctgctg ctgccgggtt tcatggccga
     2881 cgagtacacg ctgatcgccc tgaagctgtt cctgcggcag cgcggctacg acgtgcacac
     2941 gtgggggttc ggccgcaacg tcggcttcca gcgccggcac gccgccgcgc tcgagcagaa
     3001 gatccgctac atgcatcaca agagtggccg caaggtgagc ctgctcggct ggagcctcgg
     3061 cggactgttc gcgctgtacg ccgcgcacca ggcgccggag tgcgtgcgcg gggtgatcac
     3121 gctcggcagt ccggtcagca tcgacatgga cggcagccag tcaccgccgc tggtgcgggc
     3181 gctgtatcag ttgatcgcgc atccgatggg cacgagcgcg cacgcgatgc tgccgcggac
     3241 caagcagctg cgcgagcggc gcaagccgcc ggtgccgacc agctgcctgt attcgctcag
     3301 cgacggcgtc gtgccgccgc aggaggcgac gatcgaaggc gatcgccgca tgcacgagaa
     3361 cgtccgcgtg ccgggcagcc acgtcggact cggtttcaac gcgatcgtgc tgtacatcgt
     3421 cgccgaccgg ctcgcgcagc cggaaggtca gtggcgcccg ttcaggccgc aaggcgtctc
     3481 ggggaagatc taccggctga tggtgcgcga gcccgcggcg gcctgaccgc cttctttgcc
     3541 gcggcgaggt actcctggaa gctgtcgcgg atgcactgcg caaacgcttc cgggttcggc
     3601 accagttcac ggcacgaggt cggcgaaacg acgatgcgcc cgtcgtagct cgtcaccgcg
     3661 aacaccaggc ccatcccgtc ggcgatcggc atgatcgctg agaaatacgt catccgcgcg
     3721 ccgcacagat acagcggcac cagcgacccg ggcacgttgg tgatcgtgca gttggcgagc
     3781 ggcgcgcgcc gacccatgcc gagcgcgaca cgcccgagca gcttgctcgt caccgccagc
     3841 gtggacgccg gcgtgtggcg gccgatgtcg gtcagttcac gggcgccgat cgcgcgcgcg
     3901 accgtgtcgg acgcgcgcgt gtgatcgtga atcgcgcgca ggcgttcgac cgggtcggcc
     3961 agatcggtgc cgagcttcac gtgcagccag gagacctcgg gccggctgcc gggcgcggca
     4021 tcggcatcgc ggatgtagat cggcgcgatc gcggtgaggc tcgcgcgcgg cagttcctcc
     4081 tcggcctcca ggtagcgccg cagcgcgccg ccgcatacgg caagcaccac gtcgttcacc
     4141 gtggcgccct tgaccagcgc gcggatcgtc ttgaactcgt cgagcgtgaa gcggcgcgtc
     4201 tcgaacaccc gatgcggcga cacgaacgag ttgaagcgcg tgaccggcaa gcgttcgcgc
     4261 cgcatcagga tgtcgccggc caggctcagt gctgccggcg tcacccgccg gatcgcccgc
     4321 gtcaggggcg cggcgagtcg cagcggcgac atgatcgtgc tcgcgacgcc ccggcacacc
     4381 atcgcgatcg tgcccggcgg cggctccgcg aaccacggct ccggcggctc gggcggcggc
     4441 ggcgtcgtgg tgatgtcgtg cagcaggttg gtgatctcgg cctcttgctc gacgtcgatc
     4501 gcggcatggt gggtcttggt cagcagcgcg aagctgccct gtggcagatc gagaaaggca
     4561 tccagcccct cgatcacgta gatctcccac agcggacggt tcaggtcgag cgggcgcgca
     4621 tggatgcgcg aggcctggat gcagaactgg cgccagtcgc ccggcttggg ca
//
LOCUS       DBNHBY010000777         2603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_128705, whole genome shotgun sequence.
ACCESSION   DBNHBY010000777 DBNHBY010000000
VERSION     DBNHBY010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_128705"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1585)
                     /locus_tag="AC16HB_18300"
     CDS             complement(38..1585)
                     /locus_tag="AC16HB_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR5608798.1"
                     /translation="MSAVIEGVSPVLRLHPSDDVVIARRQLVSGTAVEGLTVIGLVPP
                     GHKVAVRAIAQGGPVRRYNQIIGFAKRAIRAGEHVHLHNLDIGPEHGAFARDYAFCAD
                     MKPTNYVDRPATFRGIVRADGRIATRNYIGILSSVNCSATVVRAIADRFRRDVFPQAL
                     ADFPNVDGVVALTHGTGCGMDMGEALRVLRRTLGGYARHANFGAVLVVGLGCETNQIS
                     AWLGAEGLKESAALQAFSIQDSGGTSRAVESGVARIRELLPAVNRVRREEMPVSHLML
                     GLQCGGSDGYSGITANPALGNAVDRLVAHGGTAILSETPEIYGAEHLLTRRAVSRDVG
                     EKLVARIRWWEDYTARNHGEMNNNPSPGNKAGGLTTILEKSLGAVAKGGTTNLVDVYE
                     YAQPVDKHGFVFMDTPGYDPVSATGQVAGGANMIVFTTGRGSAYGCAPSPSIKLSTNS
                     DLWRRQADDIDLNCGSIADGEAKVEEVGARIFEMIVVMASGAKSRSELHGYGQQEFVP
                     WQLGATM"
     gene            complement(1595..>2603)
                     /locus_tag="AC16HB_18305"
     CDS             complement(1595..>2603)
                     /locus_tag="AC16HB_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5608799.1"
                     /translation="AEFSVGVTAASATLGPIIPPSLPFVIYGMFANVSVGALFLAGLL
                     PGVVMAGLMMATVAFYAHKNNWGGDIGFDWGRLGKALLELAVVAAFPVAVWAAVQAGA
                     PPVLSGVVALAALLVADWRVKFDAVLPILTPVLLIGGMTAGVFTATEGAIAACVWALF
                     LGIAWYRTLRWRMLIKVSMDTIETTAIVLLIVAAASIFGWMLTATRATEAIASWVLAV
                     TSEPWTFLLLANGLMLFVGCFLEPTAAITILVPILLPIVKQLGIDPVHFGLIMVLNLM
                     IGLLHPPMGLVLFVLARVSKLSFERTTMAILPWLLPLLVSLGLVTYIPAISLWLPKIL
                     M"
BASE COUNT          460 a          922 c          832 g          389 t
ORIGIN      
        1 agcgggtgtg ttcgaaaaac gctttgcggt ggagtagtta catcgttgcc ccgagctgcc
       61 atggcacgaa ctcctgctgg ccgtacccat gcagctcgct gcgggacttt gcccccgacg
      121 ccatgacaac gatcatctcg aagatacgtg cgccgacctc ctccaccttc gcttcgccgt
      181 cggcgatgct gccgcagttc agatcgatgt cgtcggcctg gcgccgccac aggtccgagt
      241 tggtcgacaa cttgatcgac ggcgacggcg cgcagccgta cgccgagccg cgcccggtcg
      301 tgaacacgat catgttggcg ccgcccgcca cctgtccggt ggccgacacc ggatcgtagc
      361 caggcgtgtc catgaagacg aagccgtgct tgtccaccgg ctgggcgtat tcgtacacgt
      421 cgacgagatt ggtggtgccc cctttggcga ccgccccaag cgacttttcg aggatcgtcg
      481 tcaggccgcc ggccttgttg ccgggcgacg gattgttgtt catctcgcca tggttgcgcg
      541 cggtgtagtc ctcccaccac ctgatgcgcg cgaccagctt ttcgccgacg tcgcgcgaga
      601 ccgcgcgccg ggtcagcagg tgctcggcgc cgtagatctc cggcgtttcg gacaggatcg
      661 ccgtgccgcc gtgcgccacc agccggtcca ccgcgttgcc gagcgccggg ttggcggtga
      721 tgcccgagta gccatccgag ccaccgcact gcagtccgag catcagatgg ctaaccggca
      781 tctcttcgcg tcggacgcga ttgaccgccg gcagcagttc ccggatccgc gccacgccgc
      841 tttcgaccgc gcgcgacgtg ccgccgctgt cctggatgct gaacgcctgc agcgcggcgc
      901 tttctttcaa cccctccgcg ccgagccagg cgctgatctg gttggtctcg cagccgagcc
      961 cgaccacgag caccgcgccg aaattcgcgt gccgcgcata accgccgagg gtacggcgca
     1021 gcacgcgcaa tgcctcgccc atgtccatgc cgcaaccggt gccgtgcgtg agcgcgacca
     1081 cgccgtcgac gttcgggaaa tccgccagcg cctgcggaaa cacgtcgcgc cgaaagcggt
     1141 cggcaatcgc ccgcaccacc gtggccgagc agttcacgct cgacaggatg ccgatgtaat
     1201 tgcgtgtcgc gatgcgaccg tcggcacgca cgatgccgcg aaacgtggcg ggccggtcga
     1261 cgtagttcgt cggcttcatg tcggcgcaga acgcgtagtc gcgcgcgaac gcgccgtgct
     1321 ccgggccgat gtcgagatta tgcaggtgca cgtgctcgcc ggcgcggatg gcgcgcttcg
     1381 cgaagccgat gatctggttg tagcgccgca ccgggccacc ctgcgcgatc gcgcgcaccg
     1441 ccaccttgtg acccggcggc acgaggccga tcacggtcag gccctcgacg gccgtgcccg
     1501 aaacgagctg ccggcgcgcg atcacgacgt cgtccgacgg gtgcaggcgc agaaccggtg
     1561 acacaccttc gatcactgct gacatttcac ttcctcacat caggatcttc ggcaaccaca
     1621 ggctgatggc cggaatatag gtcaccagcc caaggctcac gagcagcggc agcagccacg
     1681 gcaggattgc catcgtcgtg cgctcgaacg acagcttcga cacgcgcgcg agcacgaaca
     1741 gcaccagtcc catcggcgga tgcagcagcc cgatcatcag gttcagcacc atgatcagcc
     1801 cgaagtgcac cggatcgatc ccgagctgct tgacgatcgg cagcaggatc ggcaccagga
     1861 tcgtgatcgc cgccgtgggc tccaggaagc agccgacgaa cagcatcagc ccgttggcga
     1921 gcagcagaaa cgtccacggc tcgctggtca ccgcgagcac ccaggaggcg atcgcttcgg
     1981 tcgcgcgcgt cgccgtcagc atccagccga agatcgacgc ggcggcgacg atcagcagca
     2041 cgatcgcggt ggtctcgatc gtgtccatcg acaccttgat cagcatcctc cagcgcagcg
     2101 tgcggtacca ggcgattccg aggaacagcg cccacacgca ggcggcgatc gcgccctcgg
     2161 tagcggtgaa cacccccgcg gtcatgccgc cgatgagcag cacgggagtc aggatcggca
     2221 gcaccgcgtc gaacttcacg cgccagtccg cgacgagcag cgcggcaagc gcgacgacgc
     2281 cggagagcac cggcggcgca cccgcttgca ccgcggccca gaccgcaacc ggaaacgccg
     2341 ccacgaccgc gagttccagc agcgccttgc cgagccggcc ccaatcgaac ccgatgtcgc
     2401 cgccccagtt gttcttgtgc gcgtagaacg ccacagtcgc catcatcagg cccgccatca
     2461 cgacgccggg cagcaagccg gcgaggaaca gcgcgccgac cgacacgttg gcgaacatgc
     2521 cgtagatcac gaacggcagc gacggcggaa tgatcgggcc gagcgtggcg gaggccgcgg
     2581 tcacgccgac cgagaactcg gcg
//
LOCUS       DBNHBY010000778         3889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1347308, whole genome shotgun sequence.
ACCESSION   DBNHBY010000778 DBNHBY010000000
VERSION     DBNHBY010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1347308"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2752
                     /locus_tag="AC16HB_18310"
     CDS             <1..2752
                     /locus_tag="AC16HB_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608800.1"
                     /translation="NCAPRAAVAFSGGGIRSAIVSLGLAQALARLERWRDVDYLSSVS
                     GGGYTASFLGSLFLPRKPDLAVPDPVASERLGASVEDELRVALQAAQRAQQILAADPQ
                     QQHATIDGATAIVHPLRWLRENGRYLTPGGPGDAMYMAAYYLRALLGVVYVTVIALLL
                     SALAVYLLRLGVSSVGALVWRRHSAFAWADLPEGLWVSALAWLPLAVLLGAIGPLIVA
                     YWVVYRQERRDRRTLEEWSIEASPYVLAVASLIAWTLLQAYMPGNGLHWLPAYGVWLG
                     LGAVLIRNVLLPMALTPTHGESLAWRERIARAESAVRGYRWITETEEVARARQKLTAW
                     LTTAATIALALLALATVDSLGQNAYVWWEVDRTRVGAGGAGVAALAFVVWRAAGWLVA
                     RGAVGWVAKLAPYRKPIAVVLACASLLALATIAAALIQAIVWSGGWPQPDANGIFGAT
                     ASHGWLIVGAALSIALLFSLVWLCVEFLNNSTLFRFYAARLVRTFLGAANFQRLHELS
                     EALRTQDEAAAARLVVSQSRAGDDVTLRSYYATPSGGPLHLINATLNESVSRTSNLQR
                     EDRKGLSLSIGPAGIAVQDDFWRWRRRGAASGTAGADIGSFVLPPGVADDLHPTEAQL
                     SQCERLTVGQWAAISGAAVSTGLGALSSAGFSMLIWLVNARLGYWWLPAPAMGPRVRT
                     LAMRVLKSYGLAWQEFTGAFLGRRDAYWNLSDGGHFENSAVYELIRRQVPLIFAADNA
                     ADPKYEFHDLQNLVRRARLDFGAEVRFLDAADLDERLRRWPEPLRPLRAHFGALEDFR
                     GGRESGARCALLAEIAYPRSPAQDDAAPPRRSLLVVVKPTLASFLSVDVQLYGRDHRE
                     FPQQSTADQFFDEAQWESYRRLGYEFGLRLFAAWDALAEDAQWLARRCDPVQMRSLQQ
                     RG"
     gene            complement(2766..>3889)
                     /locus_tag="AC16HB_18315"
     CDS             complement(2766..>3889)
                     /locus_tag="AC16HB_18315"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HIR5608801.1"
                     /translation="LIRAGYLPVGRDFPVFLHPTTREEYALARTERKTARGYHGFAFH
                     AAPDVTLEQDLARRDLTINAMALDESGQIIDPFGGQRDLAARVLRHVGPAFAEDPVRI
                     LRVARFAARFTEFAVADETRTQMRAMVDAGEVDALVPERVWQELARGLMERKPSRMFE
                     VLRDCGALARLLPELDRLWGVPQRADYHPEVDAGVHVMMVIDAAARLNYALPVRFAAL
                     LHDLGKGATDPALLPGHHGHEARSVELAEAVCTRLRVPGDCRDLALIVAAEHGLVLRI
                     GEMRPGTVLESLERCDALRRPERFVQFLDACEADYRGRTGKEDEPFALRTPWLAALDA
                     ARNVNAGAIAARHAGDAMRIAAAVRQARAAAIAAALGRH"
BASE COUNT          507 a         1355 c         1429 g          598 t
ORIGIN      
        1 gaactgcgcg ccgcgcgctg ccgtcgcgtt ctccggtggc ggcatccgca gcgcgatcgt
       61 gtcgctcgga ctggcgcagg cgctggcgag gctcgagcgc tggcgcgacg tcgactacct
      121 ctccagcgtg tccggcggcg gctacaccgc ttcgttcctc ggcagcctgt tcctgccacg
      181 caagcccgat ctcgccgtgc cggatccggt cgcctccgag cggctcggcg cgagcgtcga
      241 ggacgaactg cgcgttgcgc tgcaagccgc gcagcgtgca caacagatcc tcgccgccga
      301 cccgcagcaa cagcacgcga cgatcgacgg cgcgacggcc atcgtgcacc cgctgcgctg
      361 gctgcgcgag aacggccgct acctgacgcc cggcgggccg ggcgacgcga tgtacatggc
      421 cgcgtactac ctgcgcgcgc tgctcggcgt cgtgtacgtg accgtgatcg cgctgctgct
      481 gagcgcgctc gccgtctacc tgctgcggct cggcgtgagc agcgtcggtg cgctcgtgtg
      541 gcggcgccac tccgcgttcg cctgggccga cctgcccgag ggcctgtggg tgagcgcgct
      601 ggcgtggctg ccgctggcgg tgctgctggg cgcgatcggg cccctgatcg tggcttattg
      661 ggtcgtctat cggcaggagc gccgcgaccg ccgcaccctc gaagagtggt cgatcgaggc
      721 cagcccgtac gtgctcgcgg tcgcgtcgct gatcgcgtgg acgctgctgc aggcgtacat
      781 gcccggcaac gggctgcact ggctgcccgc gtatggcgtc tggctcgggc tgggcgcggt
      841 gctcatccgc aacgtgctgc tgccgatggc gctgactccg acgcatgggg agtcgctggc
      901 gtggcgcgag cgcatcgccc gcgcggagag cgcggtgcgc ggctatcgct ggatcaccga
      961 gaccgaagag gttgcgcgcg cacggcaaaa gctgaccgcg tggctgacca cagcggccac
     1021 gatcgcgctc gcgttgctcg cactcgcgac ggtcgactcg ctcggccaga acgcgtacgt
     1081 gtggtgggaa gtcgatcgca cgcgcgtcgg ggccggcggt gccggcgtag cggcgctggc
     1141 gttcgtcgtg tggcgcgcgg cgggctggct cgtcgcgcgc ggtgccgtgg gctgggtcgc
     1201 gaagctcgcg ccgtatcgca agccgatcgc ggtggtgctc gcgtgcgcct cgctgctggc
     1261 gctggcaacg atcgcggcgg cactgatcca ggcgatcgtg tggagcggcg gctggccgca
     1321 gccggatgcg aacggcatct tcggcgcgac ggcgagccac ggctggctga tcgtcggcgc
     1381 ggcgctgtcg atcgcgctgc tgttcagcct cgtctggctg tgcgtcgaat tcctcaacaa
     1441 ctcgacgctg ttccgcttct acgcggcgcg gctggtgcgc acgttcctcg gcgccgccaa
     1501 cttccagcgg ctgcatgaac tgagcgaagc gctgcgcacg caggacgaag cggcggcggc
     1561 gcggctcgtc gtgtcgcagt cgcgcgcggg cgacgacgtg acgctgcgca gctactacgc
     1621 gaccccttcg ggcgggccgc tgcacctgat caacgcgacg ctgaacgaaa gcgtcagccg
     1681 cacctcgaac ctgcagcgcg aagaccgcaa gggcctgtcg ctttcgatcg gcccggccgg
     1741 catcgcggtg caagacgatt tctggcgctg gcgtcgccgt ggcgcggcga gcggcaccgc
     1801 gggcgcggac atcggcagct tcgtgctgcc acccggcgtg gccgacgatc tccacccgac
     1861 cgaagcacaa ctgagccagt gcgaacgcct gacggtcggc caatgggcgg cgatctcggg
     1921 cgcggcggtg tcgacggggc tgggggcgct gtcgtcggcg gggttctcga tgctgatctg
     1981 gctcgtgaat gcgcggctcg gctactggtg gctgccggcg cccgcgatgg gcccgcgcgt
     2041 gcgcacgctg gcgatgcgcg tgctgaagag ctatgggctg gcatggcagg agttcaccgg
     2101 ggcgtttctc ggccggcgcg acgcgtactg gaacctgtcc gacggcggcc acttcgagaa
     2161 cagcgcggtg tacgaattga tccgacgcca ggtgccgttg atcttcgccg ccgacaacgc
     2221 cgccgatccg aagtacgagt tccacgatct gcagaacctg gtccggcgtg cgcggctcga
     2281 cttcggcgcc gaggtccgct ttctcgacgc ggccgacctc gacgagcgtc tgcggcgttg
     2341 gcccgagccg ctgcggccgc tgcgtgcgca tttcggcgcg ctcgaggatt tccgcggcgg
     2401 ccgcgagtcg ggggcgcgtt gcgcgctgct ggccgagatt gcgtatccgc ggtcgccggc
     2461 gcaggacgac gctgcgccgc cgcgccgctc gctgctcgtc gtggtcaagc cgacgctggc
     2521 gagcttcctg tcggtcgacg tgcagttgta tgggcgcgac caccgggagt tcccgcagca
     2581 gtcgacggcc gaccagttct tcgacgaggc gcagtgggag agctaccgca ggctcggcta
     2641 tgagttcggc ctgcgactgt tcgcggcgtg ggacgcgctg gccgaggacg cgcagtggct
     2701 cgcgcgccgc tgcgatccgg tgcagatgcg gtcgctgcag cagcgcggct gaggcgaccg
     2761 aacgatcagt gcctgccgag tgcagccgcg atagccgcgg cgcgtgcctg gcgcactgcc
     2821 gcggcaatgc gcatcgcgtc gcccgcatgg cgcgccgcga tcgcgccggc attcacgttg
     2881 cgcgcggcat cgagcgcggc gagccacggc gtgcgcagcg caaacggctc gtcttccttg
     2941 ccggtgcggc cgcggtaatc ggcctcgcac gcgtcgagga actgcacgaa gcgttccggc
     3001 cgccgcagcg cgtcgcagcg ttccagcgac tcgagcaccg tgccgggacg catctcgccg
     3061 atacgcagca cgagcccgtg ttccgctgcc acgatcagcg cgaggtcgcg gcaatcgccc
     3121 ggcacgcgca gccgcgtgca caccgcttcg gccagctcga ccgagcgcgc ttcgtgaccg
     3181 tgatgccccg gcagcagcgc cggatcggtc gcgcccttgc ccaggtcgtg cagcagcgcg
     3241 gcgaagcgca ccggcagcgc gtagttcagc cgcgcagcgg catcgatcac catcatcacg
     3301 tgcacgccgg cgtcgacttc ggggtgatag tcggcacgct gcggcacgcc ccacagacgg
     3361 tccagttccg gcagcaggcg cgcgagcgcg ccgcagtcgc gcagcacctc gaacatgcgc
     3421 gagggctttc gttccatcag cccgcgcgcc agttcctgcc acacgcgctc gggcaccagc
     3481 gcgtcgacct cgccggcgtc gaccatcgcg cgcatctgtg ttcgcgtctc gtcggcgacc
     3541 gcaaattcgg tgaagcgcgc ggcaaagcgc gccacgcgca gaatgcgtac cgggtcctcg
     3601 gcgaacgcgg ggccgacgtg gcgcagtacg cgcgccgcga ggtcgcgctg accgccgaac
     3661 ggatcgatga tctggccgct ctcgtcgagc gccatcgcat tgatcgtcag gtcgcgtcgc
     3721 gcgaggtcct gctccagcgt cacgtcgggc gccgcgtgga aggcgaagcc gtggtagccg
     3781 cgggccgtct tgcgctcggt gcgcgcgagc gcgtactcct cgcgcgtcgt gggatgcagg
     3841 aacaccggga aatcgcgtcc gaccggcaga tacccggcgc ggatcaggt
//
LOCUS       DBNHBY010000779         1929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_61345, whole genome shotgun sequence.
ACCESSION   DBNHBY010000779 DBNHBY010000000
VERSION     DBNHBY010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_61345"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16HB_18320"
     CDS             <1..917
                     /locus_tag="AC16HB_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5608802.1"
                     /translation="AQTFLALRGLDAEIALLADTVRLREEALALVEQRFKAGEIAELD
                     VARARTELATTRAERVALTRRRAELEHALAVLVGKAPAELDLQPQPLAFTPIAIPPGV
                     PSALLERRPDIAAAERAMAAANARIGVARAAWFPRLTLTGLLGLEAANTGDLFRASSR
                     TWALGPIAGTALALTVFDGGRRAANEQGAHAAFDETAANYQQTVLTALREVEDSLSGL
                     HMLTQQAAEQTKSVDAAQRAAALSTARYRAGYVNYLEVIDAERQVLANRRAATQVERE
                     RALATVALVRALGGGWDGSPKLAANTAP"
     gene            914..1630
                     /locus_tag="AC16HB_18325"
     CDS             914..1630
                     /locus_tag="AC16HB_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608803.1"
                     /translation="MTTARLRFDGGNCRKEQAMNASQVIQSRPGTKTTVSVVLAAWFV
                     LVYLLGAHGSFVQPPGTPPLAILFAAALPLAAFAFAWRASEAFRWLVLGADLRLLTAI
                     QEWRAGGLGFLALHVHGVLPGLFAWPAGLGDIAIGVTAPWIVLALMRDARFVAGRAFV
                     AWNLLGIVDLVVAVGAGVLGSGFVRALAGDVTTAAMAQLPLVLIPAYLVPLFVMLHVA
                     ALLQSRRARQAGHASGALSR"
     gene            complement(1620..>1929)
                     /locus_tag="AC16HB_18330"
     CDS             complement(1620..>1929)
                     /locus_tag="AC16HB_18330"
                     /EC_number="3.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR5608804.1"
                     /translation="ITQIGFTPTLRYWPSGAKAGWFVEGAIGFNAMTPLYRTREKRFS
                     TAFNFGDHIAFGYRMKEGRRWEWLVRLQHFSNAGINEPNPGENFVQLRVVVPLDLISG
                     "
BASE COUNT          243 a          649 c          729 g          308 t
ORIGIN      
        1 tggcgcagac gtttctcgcg ctgcgcgggc tcgacgccga gatcgcgctg ctcgccgaca
       61 cggtgcggct gcgcgaggag gcactggcgc tggtcgagca gcgcttcaag gcgggcgaga
      121 tcgccgaact cgacgtcgcg cgcgccagga cggaactggc gacgacgcgc gccgagcgcg
      181 tggcgctgac gcgccggcgc gccgaactcg agcacgcgct ggcggtgctg gtcggcaagg
      241 cgccggccga gctggacctg cagccgcagc cgctcgcgtt cacgccgatc gcgattccgc
      301 cgggcgtgcc gtcggcgctg ctcgagcgtc ggcccgacat cgccgccgcc gagcgcgcga
      361 tggcggcggc gaacgcgcgc atcggtgtgg cgcgtgccgc atggttcccg cggttgacgc
      421 tgacggggct gctcgggctc gaggcggcga acacgggcga tctcttccgc gcgtcgagcc
      481 gcacctgggc gctcgggccg atcgccggta ccgcgctcgc gctgacggtg ttcgacggcg
      541 ggcgccgcgc ggccaacgag cagggcgcgc acgcggcatt cgacgagacg gccgcgaact
      601 atcagcagac cgtgctgacc gcgctgcgcg aagtcgagga ttcgctttcc ggattgcata
      661 tgctgaccca gcaggcggcc gagcagacga agtcggtcga cgccgcgcag cgcgcggcgg
      721 cgctttcgac cgcgcgctat cgcgccggct acgtcaacta cctcgaagta atcgacgccg
      781 agcgccaggt gctcgccaac cggcgcgccg ccacgcaggt cgagcgcgag cgcgcgctgg
      841 ccacggtggc actggtgcgc gcactcggcg gcggctggga cgggtcaccg aaattggcgg
      901 cgaacaccgc gccgtgacca cggcgcggct gcgcttcgac ggcggcaatt gccgcaagga
      961 gcaggcgatg aacgcttccc aggtcattca gagtcgtccg ggtacgaaga cgaccgtctc
     1021 ggtcgtgctc gcggcgtggt tcgtgctggt gtacctgctc ggcgcgcacg gatcgttcgt
     1081 gcagccgccg gggacgccgc cgctggcgat cctgttcgcc gcagcgctgc cgctggcggc
     1141 gttcgccttc gcgtggcggg cgtcggaggc gttccggtgg ctggtgctgg gggccgacct
     1201 gcggctgctg accgccatcc aggagtggcg cgccggtggt ctcggattcc tggcgctgca
     1261 tgtgcatggc gttctgcccg gactgttcgc gtggccggcg ggcctgggcg acatcgcgat
     1321 cggcgtcact gcgccgtgga tcgtgctcgc gctgatgcgc gatgcgcggt tcgtcgccgg
     1381 tcgcgcgttc gtggcgtgga atctgctggg aatcgtcgat ctcgtcgtcg ccgtgggcgc
     1441 cggcgtgctg ggttccgggt tcgtgcgggc gctcgcgggc gatgtgacga cggcggcgat
     1501 ggcgcagttg ccgctggtgc tgattccggc ctacctcgtt ccgttgttcg tcatgctcca
     1561 tgtcgccgcg ctgctgcagt cgcggcgggc acggcaggcg ggtcacgcga gcggcgcgct
     1621 cagccgctga tgaggtccag cggcaccacc acgcgcagtt gcacgaagtt ctcgccggga
     1681 ttcggctcgt tgatgccggc gttggagaag tgctgcagtc gcaccagcca ctcccagcgc
     1741 cgtccttcct tcatccggta gccgaacgcg atgtggtcgc cgaaattgaa tgcggtcgag
     1801 aaacgcttct cgcgcgtgcg gtacagcggc gtcatcgcgt tgaagccgat cgcgccctcg
     1861 acgaaccagc cggctttcgc gccggagggc cagtagcgca gcgtcggcgt gaaaccgatc
     1921 tgcgtgatg
//
LOCUS       DBNHBY010000780         2227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1075799, whole genome shotgun sequence.
ACCESSION   DBNHBY010000780 DBNHBY010000000
VERSION     DBNHBY010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1075799"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC16HB_18335"
     CDS             <1..422
                     /locus_tag="AC16HB_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5608805.1"
                     /translation="GHLTAMMMAALWQVFAPDVPADLVKGGLSISGLFDLEPLLYAPF
                     VNADLMLNRERAHKLSPVRMVAATSAPVITAVGALESNEFRRQSELLGKVWRKTVVRH
                     LAVAGANHLTVCDELANPASPLFDAALDLIHRRGFPR"
     gene            508..1224
                     /locus_tag="AC16HB_18340"
     CDS             508..1224
                     /locus_tag="AC16HB_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608806.1"
                     /translation="MRRLLFTAAAAVFCTPLFAADLDTIQQLSQPQFRQLSEDLGAAL
                     SYKPLIPAEPLGVTGFDLGIAITGTQLRHRDVWQRAAGGDSVPGTLPVPTLRLHKGLP
                     LNIDVGASFATVPGSNLRYYGGELRWAILPGDTITPAVAVRGAFTKLTGVDQLDFDTR
                     SLDLSVSKGLAMFTPYAGIGNVWVKSSPSGVPLLHGEDFTQTKVYGGLNFNLAGGNLA
                     LEIDSTGSTTSFGAKLGFRF"
     gene            complement(1229..1630)
                     /locus_tag="AC16HB_18345"
     CDS             complement(1229..1630)
                     /locus_tag="AC16HB_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608807.1"
                     /translation="MTAHGVPVLRVARPTNDLVPLRAFYCDGLGFEELSEFTDHSGFD
                     GLIVGHPHAPYHLEFVRERGVTVPGAPTEEHLLVLYLPDTPQWHAAVARMEAAGFATV
                     RPHNPFWTLTGACFRDPDGYHVVLQNTAWSK"
     gene            complement(1627..1836)
                     /locus_tag="AC16HB_18350"
     CDS             complement(1627..1836)
                     /locus_tag="AC16HB_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HIR5608808.1"
                     /translation="MIKTELNLKDPDAFYEAWLDAQRGLADDESALLNARLVLLLANQ
                     IGDDRVLAECIAAAAKPFAARAGRP"
     gene            complement(1950..>2227)
                     /locus_tag="AC16HB_18355"
     CDS             complement(1950..>2227)
                     /locus_tag="AC16HB_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HIR5608809.1"
                     /translation="AVASGGGGHVQNVALLLPDGSRGDLIALLKLADHALLVLVFGEI
                     DAIDASQLEALEERYPVRFFCVRADEGSTSAFPTVIDADGHLSAQVG"
BASE COUNT          340 a          759 c          767 g          361 t
ORIGIN      
        1 gcgggcatct gaccgcgatg atgatggccg cgctgtggca ggtgttcgcg cccgacgtgc
       61 cggccgatct ggtcaagggc gggctgtcga tcagcgggct gttcgatctc gaaccgctgc
      121 tgtacgcgcc ctttgtcaat gccgacctga tgctgaaccg ggaacgcgcg cacaagctgt
      181 cgccggtgcg catggtcgcg gcgacgtccg cgccggtgat caccgcggtg ggggcgctcg
      241 aatcgaacga gttcaggcgc cagtctgagc tgctcggcaa ggtctggcgc aagaccgtcg
      301 tgcgccatct cgccgtcgcg ggcgcgaatc acctcaccgt gtgcgacgag ctcgccaatc
      361 ccgccagccc gctgttcgat gcggcgctcg acctgataca tcgccgcggg ttccctcgtt
      421 gagcttcgcc tgattgcgcg aggccgcgcg gcggcacgcg cgcgcctgcg ggcgatactg
      481 cccgctcgtt gaaactggag cattgcgatg cgaagactcc tcttcaccgc ggcggcggcc
      541 gtgttctgca cgccgctgtt tgcggccgat ctcgacacca ttcagcagct gtcgcagccg
      601 cagtttcgcc agctgtcgga ggatctcggc gctgcactgt cgtacaagcc gctgataccg
      661 gccgagccgc tcggcgtcac gggcttcgac ctcggcatcg ccatcaccgg cacacagttg
      721 cggcaccggg acgtgtggca acgcgcggcc ggaggcgaca gcgtgccggg cacgctgccg
      781 gtgccgacgt tgcggctgca caagggtctg ccattgaaca tcgacgtcgg cgcatcgttc
      841 gcaaccgtgc ccggcagcaa cctgcggtac tacggtggtg aactacgctg ggcgatcttg
      901 cccggcgaca ccatcacacc cgcggtcgcg gtgcgcggcg cgttcaccaa gctgaccggc
      961 gtggaccagc tcgacttcga cacgcgctcg ctcgacctct cggtgtcgaa gggcctggcg
     1021 atgttcacgc cgtatgccgg catcggcaat gtgtgggtga agagctcgcc gagtggcgtg
     1081 ccgctgctgc acggcgagga cttcacgcag accaaggtct atggcgggct gaacttcaat
     1141 ctcgccggcg gcaatctcgc gctcgagatc gacagcaccg gaagcacgac ctcgttcggg
     1201 gcgaagctgg ggttcaggtt ctgacgcgtc atttgctcca cgccgtgttc tgcagcacga
     1261 cgtggtagcc gtcgggatcg cggaagcagg cgccggtcaa cgtccagaac gggttgtgcg
     1321 ggcgcaccgt ggcaaacccc gccgcctcca tgcgcgcgac cgctgcatgc cactgcggcg
     1381 tgtcgggcag gtacaggacc agcaggtgtt cctccgtcgg cgcgccgggc acggtgacgc
     1441 cgcgctcgcg cacgaactcg aggtgatagg gcgcgtgcgg gtggccgacg atcaggccgt
     1501 cgaagccgct gtgatcggtg aactcgctca gctcctcgaa gccgaggccg tcgcagtaga
     1561 acgcgcgcag cggcaccaga tcgttggtcg gccgcgcgac ccgcagcacg ggcactccgt
     1621 gcgcggtcat gggcggcctg cgcgcgcggc gaacggtttc gccgcggcgg cgatgcattc
     1681 ggccagcacg cggtcgtcgc cgatctggtt ggcgagcagc agcaccaggc gcgcgttgag
     1741 caatgcgctc tcatcgtcgg ccagtccgcg ctgcgcgtcg agccacgcct cgtaaaaagc
     1801 gtccggatcc ttcaggttga gttcggtctt gatcatcgtt cgatccccag cacgatgcgc
     1861 actgcacgct cgatctcgcc ggccgacgcg tcttcgagca accccgccag gtgcaggtcg
     1921 ggccgcacca gcgcgatgtc gctgccgccc tagccgacct gcgcggacag atgaccgtcg
     1981 gcatcgatga cggtcggaaa ggcgctggtg ctgccctcgt ccgcgcgcac gcagaagaag
     2041 cgcacggggt agcgctcctc gagggcctcg agctgcgacg cgtcgatcgc gtcgatctcg
     2101 ccgaacacga ggacgagcag cgcgtgatcg gccagcttca gcagcgcgat cagatcgccg
     2161 cgcgagccgt cgggcagcag cagcgcgacg ttctgcacgt gcccgccgcc gccgctggcg
     2221 acggcga
//
LOCUS       DBNHBY010000781         3460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1347823, whole genome shotgun sequence.
ACCESSION   DBNHBY010000781 DBNHBY010000000
VERSION     DBNHBY010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1347823"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2899)
                     /locus_tag="AC16HB_18360"
     CDS             complement(<1..2899)
                     /locus_tag="AC16HB_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HIR5608810.1"
                     /translation="MSHFLDRMLFFTKERQEFADGHGVVTKEDRRWEDGYRKRWQHDK
                     IVRSTHGTNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDMPNHEPRGCARGASYSWY
                     LYSANRVKYPMVRGRLIRLWREARKSGKDPVAAWTSIVGDDAKRREYQSKRGLGGFIR
                     SSWDEVNEIIAAANAYTIKTHGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFY
                     DWYCDLPPSSPQIWGEQTDVPESADWYNSTYIMAWGSNVPQTRTPDAHFFTEVRYKGA
                     KTVAVTPDYSEVAKLSDEWLHPKQGTDAALGMAMGHVILKEFHVDKQVPYFDAYARQY
                     TDMPLLVLLKKRGDSYVADRYVRASDFADKLAQSNNPEWKTVAVDESNGQIVLPNGSI
                     GFRWGEQGGNAGKWNLEERDARTGAPLKLALSLLGKHDQVAPVAFPYFGGVQNEHFKA
                     NAFKDVLVRNVPARKLTLNGAETLVATVYDLMVANYGIDRAIPGTSQTLGGDNLARSY
                     DENAPYTPAWQEKITGVDRYAVARIAREFAQNAAKTNGKSMVIIGAAMNHWYHSDMNY
                     RAVINMLMMCGCVGQSGGGWAHYVGQEKLRPQTGWTPLAFALDWGRPPRQQNSTSFFY
                     AHSDQWRYEKLKVDEILSPLADKEKFRGTFIDFNARSERMGWLPSAPQLQTNPLQVTK
                     QAAAAGVEAKDFAVKALKDGTLKLSCLDPDNPANWPRNLFVWRSNLLGSSGKGHEYFL
                     KHLLGTSHGVLGKDLGAEGGAKPVDVKWHDDGPQGKLDLLVTIDFRMSTTCLYSDIVL
                     PTATWYEKNDLNTSDMHPFIHPLSSAVDPAWQAKSDWEIFKGIAKKFSEVCVGHLGVE
                     KDLVLTPTMHDTPSELAQPIDVKDWMKGEVDLVPGKTGPQMSVVERDYPNTFKRYTAL
                     GPLMAKVGNGGKGIAWNTQDEVQALKDLNGVVAEEGATKGLAKIDTDIDAAE"
     gene            complement(2992..>3460)
                     /locus_tag="AC16HB_18365"
     CDS             complement(2992..>3460)
                     /locus_tag="AC16HB_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608811.1"
                     /translation="WIGPFVGAAIRPVGGWISDKVGGSIVTQIISVVMVAASVAVGYV
                     MMQAYQSPTPEQYFPAFLGLFIVLFAASGVGNGSTFRTIGVIFDRTQAGPVLGWTSAI
                     AAYGAFVAPIVIGNQINAGTPQFAMYGFAVFYALCLVLNWWFYLRANAYVKNP"
BASE COUNT          533 a         1146 c         1109 g          672 t
ORIGIN      
        1 cctcggcggc gtcgatgtcg gtgtcgatct tcgccagtcc cttggtggcg ccttcctcgg
       61 cgaccacgcc attgaggtcc ttcagcgcct gcacctcgtc ctgcgtgttc cacgcgatgc
      121 ccttgccgcc attgccgacc ttcgccatca acggacccag cgcggtgtag cgcttgaacg
      181 tgttcgggta gtcgcgttcg acgaccgaca tctgcggacc ggtcttgccg ggcacgaggt
      241 cgacctcgcc cttcatccag tccttgacgt cgatcggctg cgccagttcg ctcggcgtgt
      301 cgtgcatcgt cggcgtcagc acgaggtcct tctccacgcc gaggtggccg acacagacct
      361 cggagaactt cttggcgatc cccttgaaga tctcccagtc gcttttcgcc tgccatgccg
      421 gatcgaccgc gctcgacagc gggtggatga acggatgcat gtcggacgtg ttgaggtcgt
      481 tcttctcgta ccacgtcgcg gtcggcagca cgatgtccga gtacaggcag gtcgtgctca
      541 tgcggaagtc gatcgtcacc agcaggtcga gcttgccctg tgggccgtcg tcgtgccact
      601 tgacgtcgac cggcttcgcg ccgccttcgg cgccgaggtc cttgcccagc acgccgtggc
      661 tggtgccgag caggtgcttg aggaaatact catgcccctt gcccgacgag ccgagcaggt
      721 tggagcgcca cacgaacagg ttgcgcggcc agttggcggg attgtctggg tcgaggcacg
      781 aaagtttcag cgtgccgtcc ttcagcgcct tgaccgcgaa gtctttcgct tcaacgcccg
      841 cggcggccgc ctgcttcgtc acctgcagcg gattggtctg cagttgcggc gccgacggca
      901 gccagcccat gcgctccgag cgcgcattga agtcgatgaa cgtgccgcgg aacttttcct
      961 tgtccgccag cggcgacagg atctcgtcga ccttgagttt ctcgtagcgc cactgatcgc
     1021 tgtgcgcgta gaagaaactg gtcgagttct gctgccgcgg cggccggccc cagtcgagcg
     1081 cgaacgcgag cggcgtccag ccggtctgcg ggcgcagctt ctcctgcccc acatagtggg
     1141 cccagccgcc cccgctctgg ccgacgcagc cgcacatcat cagcatgttg atgaccgcgc
     1201 ggtagttcat gtcggagtgg taccagtggt tcatcgccgc gccgatgatc accatcgact
     1261 tgccgttggt cttggccgcg ttctgcgcga attcacgcgc gatccgggcg acggcgtagc
     1321 gatcgacgcc cgtgatcttc tcctgccatg ccggcgtgta cggcgcgttt tcgtcgtagc
     1381 tcctcgcgag gttgtcgccg cccaacgtct gcgacgttcc aggaatggcg cggtcgatgc
     1441 cgtagttggc gaccatcagg tcgtagacgg tggcgaccag cgtttccgcg ccattgaggg
     1501 tcaacttgcg cgccggcacg tttcgcacca gcacgtcctt gaatgcgttg gccttgaagt
     1561 gctcgttctg cacgccgccg aagtacggga acgcgaccgg tgcgacctgg tcgtgcttgc
     1621 cgagcagcga gagcgcgagt ttcagcggcg cgccggtgcg cgcgtcgcgc tcctcgaggt
     1681 tccatttgcc ggcgttgcca ccctgttcgc cccagcggaa tccgatggag ccgttcggca
     1741 gcacgatctg gccgttcgat tcgtcgaccg cgaccgtctt ccattcgggg ttgttcgatt
     1801 gcgcaagctt gtcagcgaag tcggaggcgc gcacgtagcg gtccgccacg tagctgtcgc
     1861 cgcgcttctt cagcagcacc agcagcggca tgtcggtgta ctggcgcgca tacgcgtcga
     1921 aatagggcac ctgcttgtcg acgtggaatt ccttcaggat cacgtgcccc atcgccatgc
     1981 cgagcgcggc atcggtgccc tgtttcggat gcagccattc gtcggacagt ttggcgacct
     2041 cggaatagtc gggcgtgacc gcgaccgtct tcgcgccctt gtagcgcacc tccgtgaaga
     2101 agtgcgcgtc cggcgtgcgc gtctgcggca cgttgctgcc ccacgccatg atgtaggtcg
     2161 agttgtacca gtcggccgac tccggcacgt cggtctgctc gccccagatc tgcgggctgg
     2221 acggcggcag gtcgcagtac cagtcgtaga agctcatgca cacgccaccg atcagcgaca
     2281 gataccgcga gcccgcggcg tagctgacca tcgacattgc gggaatcggc gagaagccga
     2341 tcacgcgatc cggcccgtgc gtcttgatcg tgtaggcgtt ggcggcggcg atgatctcgt
     2401 tgacctcgtc ccacgagctg cggatgaagc cgcccagccc gcgcttgctt tggtactcgc
     2461 gccgcttggc gtcgtccccg acgatcgacg tccacgcggc gacggggtcc ttcccgctct
     2521 tgcgcgcctc gcgccacagg cggatcaggc gcccgcgcac catcgggtac ttcacgcggt
     2581 tggccgagta caaataccag gagtaactcg cgccgcgtgc gcagccgcgc ggttcgtgat
     2641 tcggcatgtc ggggcgcgtg cgcgggtagt cggtctgctg cgtctcccac gtgacgatgc
     2701 cgcctttcac gtagatcttc cacgagcacg atccggtgca gttggtgccg tgcgtggagc
     2761 gcacgatctt gtcgtgctgc cagcgcttgc ggtagccgtc ttcccagcgg cggtcctcct
     2821 tcgtcacgac gccatggccg tcggcgaact cctgccgctc cttcgtgaag aacagcatgc
     2881 ggtcgagaaa atgactcatc tcttgctccg tgatttgtga tgcgtgattc gctgctcgcg
     2941 ccgatcctat gcgacgcgcg acccggcagg tcgcgcgtca ccgtcaaagg gtcacgggtt
     3001 cttcacgtac gcattggcgc gtagatagaa ccaccagttc agcaccaggc acagcgcgta
     3061 gaacaccgcg aagccgtaca tggcgaactg cggcgtgccg gcattgatct gattgccgat
     3121 cacgatcggc gcgacaaagg cgccatacgc ggcgatcgcc gacgtccagc ccagcaccgg
     3181 gccggcctgg gtacggtcga agatcacgcc gatggtgcgg aacgtggagc cgttgccgac
     3241 gccgctggcg gcgaacagca cgatgaacag gccgaggaat gccgggaaat actgctccgg
     3301 cgtgggcgac tggtaggcct gcatcatcac gtagcccact gcgaccgagg ctgccaccat
     3361 gaccaccgag atgatctggg tgacgatcga tccgccgacc ttgtcggaga tccacccgcc
     3421 gaccgggcgg atcgccgccc cgacgaaggg gccgatccat
//
LOCUS       DBNHBY010000782         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1281135, whole genome shotgun sequence.
ACCESSION   DBNHBY010000782 DBNHBY010000000
VERSION     DBNHBY010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1281135"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1257
                     /locus_tag="AC16HB_18370"
     CDS             262..1257
                     /locus_tag="AC16HB_18370"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5608812.1"
                     /translation="MRRGARKSPDSGYRIRCLIVWKLAKGQSSPEVAEALEVARATVS
                     RVAKRFRTDSVAGLVDRREDNGRAKVTDEVLAILERLVAGTPQEYGWCRPTWTRELLA
                     RELAAQTGVGVCIRTIARWLAALGARWGRGRPVVLCPWARRKRARRLGVLRRIVAATT
                     SAEPVFYVDELDIHLNPRVGCDWMLRRTQKVVTTPGANVKRFVAGALHAGTGRMAWVT
                     ATHKRSGLFIALLHELRRRYRRARRIHLVLDNYAIHASRQTKAALAEFGDRFALHFLP
                     PFSPDANRIERLWRDVHAAVTRHHRCRTIEELLAAVEAFLRAVGRGGDPQLMRAA"
     gene            1290..>1501
                     /locus_tag="AC16HB_18375"
     CDS             1290..>1501
                     /locus_tag="AC16HB_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608813.1"
                     /translation="MPIHDQSYRHYSGTREPSGRAWLIIASAGIRSMIRKRRFLGLLL
                     LAYLPFLVYAIRFYLTANVAVTQVSSL"
BASE COUNT          245 a          478 c          510 g          268 t
ORIGIN      
        1 tatgcggccc aacacgctcg ccgtttgtgc gccgccccga accttgcgtt tgcataagcg
       61 cttggtcaat acgtttcccg aagactaaat aagggggcgc gattccggat ctaaaatcac
      121 gcggcccggt gaatcgccgg tacacgaaag cgggcacgca tccgtattct ctgcaaaggg
      181 gttcggcggc cagcggaacc cagagaaacg gaggtgcccg tgacgttggg tataacactg
      241 catgggcgtg cgaagcaact cgtgcgacgc ggcgcccgga aatcgccgga cagcggctat
      301 cgcatccgct gtctgatcgt gtggaagctg gcgaaggggc agtcgtcgcc agaagtcgcc
      361 gaggcgcttg aagtcgcccg ggcgaccgtc agccgcgtgg cgaagcgatt tcgcacggac
      421 agcgttgccg gtctcgtcga tcgccgggag gataacggac gcgccaaggt gacggacgag
      481 gtgctcgcga tacttgagcg cctggtggcg ggcacgccgc aagagtacgg ctggtgccgt
      541 cccacgtgga cgcgcgagtt gctggcccgc gagctggcgg cacagacagg ggtcggcgtc
      601 tgcattcgga cgatcgcgcg gtggttggcg gcgcttggcg cccgctgggg tcgtggccgg
      661 ccggtggtgc tgtgcccgtg ggcccggcgc aagcgagccc ggcgtctcgg cgtgctgcgt
      721 cgcatcgtcg ccgcgaccac cagcgcggag ccggtgttct acgtggacga gttggatatc
      781 cacctgaatc cgcgagtggg ctgcgactgg atgctgcgac ggacccaaaa ggtggtgacg
      841 acgccgggag cgaacgtgaa acgcttcgtc gccggggcgt tgcacgccgg cacgggccgg
      901 atggcctggg tgacggcgac gcacaagcgc agcggcctgt tcatcgcgct gctgcacgag
      961 ttgcgccggc gctaccgtcg ggcgcgacgc attcacctgg tgcttgataa ctacgccatc
     1021 cacgcgagtc gccagacgaa ggcggcgctc gcggagttcg gcgaccggtt cgccttgcac
     1081 ttcctgccac cgttctcgcc cgatgccaat cgcatcgagc gactgtggcg cgacgtgcat
     1141 gcggcggtca ccagacatca tcgctgtcga accatcgagg agttgctggc cgccgtcgaa
     1201 gcgttccttc gagcggtggg ccgaggcggc gacccgcaac tgatgcgggc cgcctgagtg
     1261 ttaccgaatc gcgatctctt atttagacca tgcccattca cgatcagagt tatcgccact
     1321 acagcggcac ccgcgaaccg tccgggcgcg cgtggctcat catcgcctcc gcgggcatcc
     1381 gttcgatgat ccgcaagcgc cgttttctcg gcttgttgct gctcgcctat ctaccgttcc
     1441 tcgtctacgc catccgcttc tatctgacgg ccaacgtcgc cgtcacgcag gtttcgtcgc
     1501 t
//
LOCUS       DBNHBY010000783         1605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1211564, whole genome shotgun sequence.
ACCESSION   DBNHBY010000783 DBNHBY010000000
VERSION     DBNHBY010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1211564"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16HB_18380"
     CDS             complement(<1..402)
                     /locus_tag="AC16HB_18380"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR5608814.1"
                     /translation="MKHPRITLIAAVARNGVIGSAGTIPWRIPGDLPRFKRITLGHPV
                     IMGRRTWESLGRPLAGRRNLVISRTLGFAPGGAEVFVSLDAALAACADAGEVFVIGGT
                     EAYRDALPRARRLLLTEIDADVDGDAHFPPFD"
     gene            complement(399..1271)
                     /locus_tag="AC16HB_18385"
     CDS             complement(399..1271)
                     /locus_tag="AC16HB_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5608815.1"
                     /translation="MLKRRALIGLAALTLLWGVNWPIMKFSLRELTPIYFRGVTMSGG
                     ALLLFAFYTLRGTSMRLPRAAWLPLAWLAAPNIFGWHLFSILGVQELASGRAAILGFT
                     MPIWTTLLAIVFFGERLTPRVAVSALCALAAVGLLLANELTALAGRPRGVLWMQIAAF
                     CWALGTILMRRTTLRLPTPVITVWMMALSAAAFWIVAPLFEPWPAWRFSPWMWIALAW
                     GAAVNYGVAQIFWFGLARELPPTASAFSVMAVPIVGIVTAIPLVGELPRWQDFAAAVF
                     VIAAIAAALLRRPT"
     gene            complement(1265..>1605)
                     /locus_tag="AC16HB_18390"
     CDS             complement(1265..>1605)
                     /locus_tag="AC16HB_18390"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580070.1"
                     /note="catalyzes the formation of 3-methyl-2-oxobutanoate
                     from 2,3,-dihydroxy-3-methylbutanoate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5608816.1"
                     /translation="IPLKLARAGVKDMVRISDARMSGTAFGTIVLHVSPEAAVGGPLA
                     LVRSGDRIRLSVQTRRIDLRVDESELARRRAALVSRAPAPARGYAKLYLDHVLQAEDG
                     CDFDFLRTAC"
BASE COUNT          281 a          588 c          553 g          183 t
ORIGIN      
        1 gtcgaacggc ggaaagtgcg cgtcgccgtc gacgtcggcg tcgatctcgg tgagcagcag
       61 ccgccgcgcg cgcggcagcg catcgcggta ggcctcggtg ccgccgatca cgaacacctc
      121 gccggcatcg gcgcaagcgg caagcgcggc gtcgagcgaa acgaacacct ccgcgcctcc
      181 cggcgcgaac cccaacgtgc gcgagatcac gaggttgcgc cgccccgcca gcggccggcc
      241 aagcgactcc cacgtcctgc gccccatgat caccgggtgg cccagcgtga tgcgcttgaa
      301 ccggggcagg tcgccgggaa tgcgccacgg gatcgtgccc gcgctgccga tcacgccgtt
      361 gcgggcgaca gcggcgatca gcgtgatgcg cggatgtttc atgtcggtcg tctgagcaga
      421 gcggcggcga ttgccgcgat cacgaacacg gcggccgcga agtcctgcca gcgcggcaac
      481 tcacccacga gcggaatcgc ggtgacgata ccaacgatcg gcaccgccat caccgagaac
      541 gcgctggcgg tcggcggcag ttcgcgcgcc agcccgaacc agaagatctg cgccacgccg
      601 tagttgaccg ccgcgcccca cgccagcgcg atccacatcc agggcgagaa gcgccacgcc
      661 ggccacggct cgaagagcgg tgcgacgatc cagaacgcag ccgccgacag cgccatcatc
      721 cacaccgtga tcaccggcgt cggcaggcgc aacgtggtcc gccgcatcag gatcgtgccg
      781 agcgcccagc agaacgcggc gatctgcatc cacagcacgc cgcgcggccg gccggcgagc
      841 gcggtcagtt cgtttgccag cagcaggccg accgccgcga gcgcacacag cgcgctgacg
      901 gccacccgcg gcgtcagcct ttcgccgaag aagacgatcg cgagcagcgt ggtccagatc
      961 ggcatcgtga agccgaggat cgccgcgcgt cccgacgcca gctcctgcac gccgaggatc
     1021 gagaacaggt gccagccgaa gatgttggga gccgcgagcc acgcgagcgg cagccatgcg
     1081 gcgcgcggca gccgcatcga cgtgccgcgc agcgtgtaga acgcgaagag cagcaacgcg
     1141 ccgccgctca tcgtcacgcc gcggaagtag atgggcgtca gctcccgcag cgagaacttc
     1201 atgatcggcc agttcacgcc ccacagcagc gtgagcgcgg ccagcccgat cagcgcgcgc
     1261 cgcttcagca cgccgtgcgg aggaagtcga agtcgcagcc gtcctcggcc tgcagcacgt
     1321 ggtcgaggta gagcttcgcg tagccgcgtg cgggcgcggg cgcgcgcgaa accagcgcgg
     1381 cacggcggcg cgcgagttcg gactcgtcga cccgcaggtc gatccgacgc gtctgcacgc
     1441 tcaggcggat gcggtcgccg cttctcacca gcgccaatgg cccgccgacg gccgcctccg
     1501 gcgacacgtg cagcacgatg gtgccgaacg cggtgccgct catgcgcgcg tccgagatcc
     1561 gcaccatgtc cttcacgccg gcgcgcgcga gcttcagcgg gatcg
//
LOCUS       DBNHBY010000784         3722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1281250, whole genome shotgun sequence.
ACCESSION   DBNHBY010000784 DBNHBY010000000
VERSION     DBNHBY010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1281250"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16HB_18395"
     CDS             complement(<1..506)
                     /locus_tag="AC16HB_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HIR5608817.1"
                     /translation="MFHRLFAILTAALFAGCSSIDPQVYAQQQPALDLQRYFSGGLVG
                     HGAVMSRAGEVQRRFVVTLKGSWRDGVGTLEEDFVWSDGKQERRVWTLRPAGAPEKWT
                     GTAADVVGAATGTVSGNALNWTYTFALKTDGGRTFNIAFDDWMFLIDEQVLLNRAVMS
                     YFGVRVGE"
     gene            complement(514..1794)
                     /locus_tag="AC16HB_18400"
     CDS             complement(514..1794)
                     /locus_tag="AC16HB_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5608818.1"
                     /translation="MSAADTTVGLRPIAMNGLLAHAALGLPLAMMALPVYVLVPRFYA
                     DETGLALATIGALLLGTRLLDACADPLLGAWVDAHRRTGSYARPILIACAPLALGFAL
                     LFSPPADWTPNATALWLALTLTASYLGYSLASIAYQSWGSELARDDAGRTRITAAREG
                     AGLIGVVLAAALPQALGIPSLILLFLATLALALFFLLRRSPHPAPHAGTRGAHGLTGI
                     AVPLGSPRFRWLLTVFVSNGIAAAIPATLVLFFIADRLQLEHHSGLFLALYFIAGAAS
                     MPLWVRAAARFGLHAAWLGGMVAAVAAFVWAYALASGDLAAYAVICALSGAALGADLA
                     LPPALLARVIDANGHRGTREGAYFGLWNFVNKLNLALAAGLALPLLGAIGYAPGSRDP
                     VALHALSFTYAVVPCLLKLAAGALLLFAWRQRRF"
     gene            complement(1791..2351)
                     /locus_tag="AC16HB_18405"
     CDS             complement(1791..2351)
                     /locus_tag="AC16HB_18405"
                     /inference="COORDINATES: protein motif:HMM:NF027363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR5608819.1"
                     /translation="MSRSTRWFTPARVVAAALALHAGAAAATPALVRNYITDAQVAGP
                     GRLTWFGFHVYDARLYVPRGYDARAPLAQPFVLELQYARALKGRAIADASLDEIARLR
                     FGNDVQRRHWHARMVELFPDVDAGRTLAGVHLPGVGARFYFEGRFIGAVDDPEFARAF
                     FSIWLDERTRAPQLRATLFEQLGAQR"
     gene            complement(2317..3546)
                     /locus_tag="AC16HB_18410"
     CDS             complement(2317..3546)
                     /locus_tag="AC16HB_18410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5608820.1"
                     /translation="MNEFALTPTPAASIALPLRQRWLLHLLARLDRGSLHLRFPDGQQ
                     ALFGSGAPCADLTLANWNPLAAALRSGDIGFAESYVAGDWSTSDLPRLLALFVRNRNA
                     AEQVIYGSSAGRLWYRIRHWMNRNTRAQSRKNIHAHYDLGNDFYALWLGRSMTYSSAL
                     FDEAHDPHRPASGAELEAAQSAKYARVLHELALAPAVRVLEIGCGWGEFAATAARAGQ
                     RVTGLTLSPSQLSFAGERLARDGLDATLKLQDYRDERGRYDGIASIEMFEAVGEAYWP
                     AYFATLKRCLAPGGRACVQTIEITDALFDRYRRGTDFIQQYIFPGGMLPSPSAFAAHA
                     RRAGLAIVNAHSFGRHYARTLATWRTQFAAQETTVRALGFDERFIRLWNFYLAYCEAA
                     FAEGNTDVTQYTLVHAG"
     gene            complement(3543..>3722)
                     /locus_tag="AC16HB_18415"
     CDS             complement(3543..>3722)
                     /locus_tag="AC16HB_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HIR5608821.1"
                     /translation="ARLARTLITHPAFTVGVIARIHWQALRLWFKRVAFNSRPRPPAY
                     GVTRSNTDPPSATAP"
BASE COUNT          630 a         1262 c         1354 g          476 t
ORIGIN      
        1 atctcgccga cgcgcacccc gaagtacgac atcaccgcgc ggttcagcag cacctgctcg
       61 tcgatcagga acatccagtc gtcgaacgcg atattgaacg tgcggccgcc gtcggtcttc
      121 agcgcgaacg tgtaggtcca gttgagcgcg ttgccactga ccgtgccggt ggccgcaccg
      181 accacgtcgg ccgccgtgcc ggtccacttt tccggcgcac cggccggccg caaggtccag
      241 acgcgccttt cctgcttgcc gtcggaccag acgaagtcct cctcgagcgt gccgacgccg
      301 tcgcgccagc ttcccttgag cgtgaccacg aagcggcgct gcacctcgcc cgcacggctc
      361 atcaccgcgc cgtggccgac caggccgccg ctgaaatagc gctgcaggtc gagcgccggc
      421 tgctgctgcg cgtagacctg cgggtcgatt gacgaacagc cggcgaacag cgcggcggtc
      481 aggatcgcga acagtcggtg gaacatgggg acctcagaag cgccgctggc gccaggcgaa
      541 gagcagcagc gcgcccgcgg ccagcttgag cagacacggt acgaccgcgt aggtgaacga
      601 cagcgcgtgc agcgcgaccg gatcgcggct gccgggggcg tagccaatcg cgccgagcag
      661 cggcaacgcg aggccggcgg ccagcgcgag attcaacttg ttgacgaagt tccacaggcc
      721 gaagtacgcg ccttcgcgcg tgccacgatg gccgttggcg tcgatcaccc gcgccagcag
      781 ggccggcggc agcgccagat cggcgccgag tgccgcaccc gacagcgcgc agatgacggc
      841 ataggcagcg aggtcgccgc tcgcgagtgc gtaggcccag acgaacgcgg cgacggccgc
      901 gaccatgccg ccgagccagg ccgcgtgcag cccgaagcgc gccgcggcgc gcacccacag
      961 cggcatgctc gcggcgccgg cgatgaagta cagcgcgagg aacaggcccg agtggtgctc
     1021 gagttgcagc cgatcggcga tgaagaacag caccagcgtg gccgggatcg ccgcggcaat
     1081 gccgttgctg acaaacacgg tcagcagcca gcgaaagcgc ggcgagccga gcggcaccgc
     1141 gatgccggtg aggccgtgcg ccccgcgtgt gccggcgtga ggcgccggat gcggcgatcg
     1201 gcgcagcaag aaaaagagcg cgagcgccag cgtggcgaga aacagcaaga tcagcgacgg
     1261 tatgccgagc gcctgcggca gggcggcggc gagcacgacg ccgatcagcc ccgcgccctc
     1321 gcgcgcagcg gtgatgcgcg tccgtcccgc gtcgtcgcgt gcgagttccg acccccacga
     1381 ctggtaggcg atcgacgcca ggctgtagcc gaggtagctg gcggtcagcg tgagcgccag
     1441 ccacagcgcg gtcgcattcg gcgtccagtc ggcgggcgga ctgaagagca gcgcgaaccc
     1501 gagcgccagc ggcgcgcacg cgatcaggat cggccgcgca tagctgccgg tccggcggtg
     1561 agcgtcgacc catgcgccga gcagcgggtc ggcgcatgcg tcgagcaggc gcgtgccgag
     1621 cagcagcgcg ccgatcgttg ccagtgccag ccccgtctcg tccgcataga agcgcggcac
     1681 cagcacgtag accggcagtg ccatcatcgc cagcggcagg ccgagcgcgg cgtgcgcgag
     1741 cagcccgttc atggcgatcg gccgcagtcc gaccgtcgta tcggcggcgc tcatcgctgc
     1801 gcgccgagtt gctcgaacag ggtcgcgcgc agttgcggtg cgcgcgtacg ctcgtcgagc
     1861 cagatcgaaa agaacgcgcg cgcgaactcg ggatcgtcga cggcgccaat gaagcggcct
     1921 tcgaagtaga agcgcgcgcc gacgcccgga agatgcacgc cggccagcgt ccgtccggca
     1981 tcgacatcgg gaaagagctc gaccatgcgc gcgtgccagt gccggcgctg cacgtcgttg
     2041 ccgaagcgca ggcgagcgat ctcgtcgagg ctggcgtcgg cgatcgcacg ccccttcagc
     2101 gcgcgcgcgt actgcagttc gagcacgaac ggctgggcga gcggcgcgcg cgcgtcgtag
     2161 ccgcgcggca cgtacaggcg ggcgtcgtag acgtggaagc cgaaccaggt caggcgaccg
     2221 ggcccggcga cctgcgcgtc ggtgatgtaa ttgcgcacaa gtgccggggt cgcagccgcg
     2281 gcgcccgcat gcagcgccag cgctgccgcg acaaccctag ccggcgtgaa ccagcgtgta
     2341 ctgcgtgaca tcggtgttgc cttcggcgaa cgcggcctcg cagtacgcga gatagaaatt
     2401 ccacagccgg atgaagcgct cgtcgaagcc gagcgcgcgc accgtggtct cctgcgcggc
     2461 gaactgcgtg cgccacgtcg ccagcgtgcg tgcgtagtgg cggccgaacg agtgcgcgtt
     2521 gacgatcgcg agcccggcgc gtcgcgcgtg cgcggcgaag gcgctcggcg acggcagcat
     2581 gccgccggga aagatgtact gctggatgaa gtcggtgccg cggcgatagc ggtcgaacag
     2641 cgcatcggtg atctcgatcg tctgcacgca cgcgcgtccg ccgggcgcga ggcagcgctt
     2701 cagcgtggcg aagtaagccg gccagtacgc ctcgccgacg gcctcgaaca tctcgatcga
     2761 cgcgatgccg tcgtagcgac cgcgttcgtc gcggtagtcc tgcagtttga gcgtggcgtc
     2821 gagcccgtcg cgcgcgaggc gctcgcccgc aaacgataac tgcgacggcg acagcgtcag
     2881 ccccgtcacg cgctggccgg cgcgcgccgc ggtggccgcg aactcgcccc agccgcaccc
     2941 gatctcgagc acgcgtacgg ccggcgccaa cgcgagttca tgcagcacgc gcgcgtactt
     3001 cgcgctctgg gcggcctcga gttcggcgcc actggcaggg cggtgcggat cgtgcgcctc
     3061 atcgaagagc gcgctggagt aggtcatcga ccggccgagc cagagcgcat agaagtcgtt
     3121 gccgaggtcg tagtgagcat ggatgttctt cctcgactgc gcccgcgtgt tgcggttcat
     3181 ccagtggcgg atgcgatacc agagccggcc cgcgctgctg ccgtagatca cctgctcggc
     3241 cgcgttccgg ttgcgcacga acaatgccag caggcgcggc agatcggacg tcgaccagtc
     3301 gcccgcgacg tacgactcgg caaagccgat gtcgccgctc ctgagcgcgg cggcgagcgg
     3361 gttccagttc gccagcgtga ggtccgcgca cggtgccccc gatccgaaca gcgcctgctg
     3421 cccgtcggga aagcgcagat gcagcgagcc gcgatcgagg cgtgccagga gatgcagcag
     3481 ccagcgctgg cgcagcggca gcgcaatcga ggcggcaggt gtgggggtaa gggcgaactc
     3541 gttcatggcg cagtggcgga gggtggatcg gtattggagc gcgtgacgcc gtacgcgggc
     3601 ggccgcggcc gggagttgaa ggcgacgcgc ttgaaccaca ggcgcagtgc ctgccaatga
     3661 atgcgcgcga tcacgccaac agtgaacgcc ggatgcgtga tcagcgtgcg cgcgagccgt
     3721 gc
//
LOCUS       DBNHBY010000785         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1348086, whole genome shotgun sequence.
ACCESSION   DBNHBY010000785 DBNHBY010000000
VERSION     DBNHBY010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1348086"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16HB_18420"
     CDS             <1..265
                     /locus_tag="AC16HB_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR5608822.1"
                     /translation="MPVLVQALPGMESMATMMMKNKMKSQGVASLEDLRNVCIESDVK
                     FIGCQMTVDLFEFSKDDFIPQTQFGGAAMFLGFAGDTDISLFI"
     gene            419..886
                     /gene="soxY"
                     /locus_tag="AC16HB_18425"
     CDS             419..886
                     /gene="soxY"
                     /locus_tag="AC16HB_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR5608823.1"
                     /translation="MSESRRDFIKLTGVFGALAAIGALSADEALAQQAAWNKAAFETK
                     SLNDTVKALGGAGAAQSADIAITAPDIAENGAVVPIGITSKIPNTQAVYIMVEKNPNT
                     LAAGFHIPAGTEPNVTTRIKMGQTSNVHAVVKAGDKFFVATKEVKVTLGGCGG"
     gene            907..1218
                     /gene="soxZ"
                     /locus_tag="AC16HB_18430"
     CDS             907..1218
                     /gene="soxZ"
                     /locus_tag="AC16HB_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR5608824.1"
                     /translation="MADPMRIRATAQPDGVEVRVLMSHEMETGQRRDGSGNLVPAHFI
                     QTVTVTHAGKTVLSADWGPAVAKNPYMQFKFQGGKKGDEIVVTWVDNKGETRTDKAII
                     N"
     gene            1261..2121
                     /gene="soxA"
                     /locus_tag="AC16HB_18435"
     CDS             1261..2121
                     /gene="soxA"
                     /locus_tag="AC16HB_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR5608825.1"
                     /translation="MVGRTMRVKPGGIVGKRVAFELRIGALVLVAAAAFGAAAQDKTI
                     QEIERYRAMLQDGNPAELVSARGEELWKEKRGPKKASLEQCDLGLGAGVVKGAYVRLP
                     RYFADVDQVMDFEARLVHCMVTLQGFDRAAATKNPFSGAGQRQTDIEALTAYVVEQSR
                     GMTIAVPNEHPKEKAAYDRGKTLFYMRAGPYDFACSTCHGVDNQRIRLQDLPNFEKAE
                     PAQRAFATWPAYRVSQGALRTMQWRLNDCFRQARWPEPTFISQGTIDLITFLGVKANG
                     GKMDSPSIKR"
     gene            2133..>2466
                     /locus_tag="AC16HB_18440"
     CDS             2133..>2466
                     /locus_tag="AC16HB_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR5608826.1"
                     /translation="MRRHLLIAVVAALAASGCAAWRSWTYAPPSRDETVAVIKSSFHD
                     RGIAKVDRLNQTELQAVCSDTHNNPPKDVRARLEQGALASVKYPGDGKWLGDFKAGER
                     IAQTGVGLQ"
BASE COUNT          493 a          760 c          847 g          366 t
ORIGIN      
        1 gatgccggtg ctggtgcagg ccctgccggg catggaatcg atggcgacga tgatgatgaa
       61 gaacaagatg aagtcccagg gcgtggcctc gctcgaggac ctgcgcaacg tctgcatcga
      121 atccgacgtg aagttcatcg gctgccagat gacggtcgat ctgttcgagt tcagcaagga
      181 cgacttcatt ccgcagaccc agttcggcgg cgcggcgatg ttcctcggct ttgccggcga
      241 caccgacatc agtctgttca tctagcgccg ctggatggcg cggcagccga aggaagcgga
      301 tcggggcgac cttacggtcc aaccctgtac cccatcagaa ggagtgatcc ctgtcgggcg
      361 gcctgcttaa cattcgccga ttgcaggggg attgcacatg acgctaggag acgatccaat
      421 gagtgaatcg aggcgtgact tcatcaaact caccggcgtc ttcggcgcgc tggcggcgat
      481 cggcgcgctc agcgcggacg aggcgctggc acagcaggcg gcctggaaca aggccgcgtt
      541 cgagaccaag agcctgaacg acacggtcaa ggcgctcggt ggcgccggcg ccgcgcagag
      601 cgcggacatc gcgatcacgg cacccgacat cgccgagaac ggcgcggtcg ttccgatcgg
      661 catcacgagc aagattccca acacgcaggc cgtctacatc atggtcgaga agaatccgaa
      721 cacgctcgcc gccggcttcc acattcccgc gggaacggag ccgaacgtga cgacgcggat
      781 caagatgggc cagacgtcga acgtgcatgc ggtcgtgaag gccggcgaca agttcttcgt
      841 cgccacgaag gaagtcaagg tcacgctcgg cggctgcggc ggctgagcgg aaacgaggag
      901 accggtatgg cagatccgat gcgaattcgc gccacggcgc agcccgacgg agtcgaggtg
      961 cgcgtgctga tgagtcacga gatggaaacc gggcagcgtc gcgacggcag cggcaacctc
     1021 gtgcccgcgc atttcatcca gaccgtgacc gtcacgcatg ccggcaagac ggtgctgtcc
     1081 gccgattggg gtccggcggt agccaagaac ccgtacatgc agttcaagtt ccagggcggc
     1141 aagaagggag acgagatcgt cgtcacgtgg gtcgacaaca agggagaaac ccggaccgac
     1201 aaggccatca tcaactgacc ctccggtgat cggcgcagaa acactcaagg agactccctc
     1261 atggttggac gaacaatgcg cgtgaagccc ggcggcatcg ttgggaaacg agttgcattc
     1321 gaactgcgga tcggcgccct cgtgcttgtc gccgcggcgg cgttcggcgc cgccgcgcaa
     1381 gacaaaacga tccaggagat cgagcgctac cgcgcgatgc tgcaggatgg caacccggcg
     1441 gaactcgtct ccgcgcgcgg cgaggagttg tggaaggaga agcgcggtcc gaagaaggcg
     1501 tcgctcgagc agtgcgacct gggcctcggg gccggcgtgg tcaagggcgc gtacgtgcgg
     1561 ctgccgcgct actttgccga cgtggaccag gtgatggact tcgaggcgcg cctggtccac
     1621 tgcatggtga cgctgcaggg cttcgatcgc gccgcggcga ccaagaaccc attttccggc
     1681 gcgggccagc ggcagacgga catcgaggca ctcacggcct acgtggtcga gcagtcgcgc
     1741 ggcatgacga tcgcggtgcc gaacgaacat ccgaaggaaa aggccgccta cgaccgcggc
     1801 aagacgcttt tctacatgcg tgcgggcccg tatgacttcg cctgctcgac ctgtcacggt
     1861 gtggacaacc agcgcatccg cctgcaggac ctgccgaact tcgagaaggc cgagccggcg
     1921 cagcgcgcct tcgcgacctg gcccgcgtat cgcgtctcgc agggggcgct gcgcacgatg
     1981 caatggcggc tgaacgactg cttccggcag gcgcgctggc ccgagccgac cttcatttcg
     2041 caggggacga tcgacctgat cacgttcctc ggcgtgaagg cgaacggcgg caagatggac
     2101 tcgccgtcga tcaagcgata ggagacggcc gaatgagacg acatcttctg atcgcagtcg
     2161 tggcggcgct tgccgcgagc ggttgtgccg catggcggtc gtggacgtac gcgccgccca
     2221 gccgcgacga aacggtggcg gtgatcaagt cgagcttcca cgaccgcggc atcgccaagg
     2281 tcgatcgact gaaccagacc gaactgcagg cggtgtgctc cgacacgcac aacaacccgc
     2341 cgaaggacgt gcgcgcgcgc ctggaacagg gggcgctggc gagcgtcaag tatccgggcg
     2401 acggcaagtg gctcggcgac ttcaaggccg gcgagcggat cgcgcagacc ggcgtcggcc
     2461 tgcagt
//
LOCUS       DBNHBY010000786         1863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1415349, whole genome shotgun sequence.
ACCESSION   DBNHBY010000786 DBNHBY010000000
VERSION     DBNHBY010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1415349"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16HB_18445"
     CDS             <1..183
                     /locus_tag="AC16HB_18445"
                     /inference="COORDINATES: protein motif:HMM:NF015882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR5608827.1"
                     /translation="TTEADLAQGSLYPPLNQVRDVSARIAVEVAEVAYRDGLAELPRP
                     ADLGEAIRAEMYDPGY"
     gene            complement(436..>1863)
                     /locus_tag="AC16HB_18450"
     CDS             complement(436..>1863)
                     /locus_tag="AC16HB_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5608828.1"
                     /translation="RDRLVYEEPDDSFYLGVETTKDRRYLLIHSSSTITSEVRYARAD
                     DLGLDFKVLLPRDRGHEYTVDHVDDRWIIRSNWQARNFRILEAATDAAGDRANWREVV
                     PHREDVLIADFDVFTGFLALEERTQGLRKVRIRPWHGGRDFHLDSDEAAYRMALGANE
                     EIDSEVVRYTYTSLTTPPTTYDYDLRNGERTLLKREPVLGDFDPSRYATEQLWAVARD
                     GERVPVVIAYRRDRYERNGSAPLLQYGYGAYGISSDPLFSSSLLSLLDRGFLYAIAQV
                     RGGQELGRRWYEQGRLLEKRHSFEDFIDVTRFLVAEGYADPGRVFARGGSAGGLLVAA
                     VANMAPELYRGMVAHVPFVDVVTTMLDESIPLTTNEYDEWGNPGEREYYAYMLSYSPY
                     DNVAAHAYPALYVTTGLWDSQVQYYEPAKWVARLRRFRTDDRVLLLRVNMEAGHGGKS
                     GRFEHLRELAEEYAFLIDQAGLAEP"
BASE COUNT          303 a          622 c          629 g          309 t
ORIGIN      
        1 acgaccgagg ccgacctcgc acaaggcagc ctttatccgc cgctcaacca ggtgcgcgac
       61 gtgtcggcgc gcattgcggt cgaagtcgcc gaggtcgcat atcgcgacgg cctcgccgaa
      121 ctgccgcgcc ctgcggacct cggcgaggcg atccgtgccg agatgtacga ccccggctac
      181 tgagccacgc gggcgcgccg gaacctcgtt acgaagagac tgagcgcggg atcagggtca
      241 ggcgacacag cgccgacggc gcttccgagg cgtgcgggct tcaggtccgt ccggcaaaag
      301 cttctcgagg caggagccga gagcgagcct acagggacgt acttgcggcg tctcttgccg
      361 gacagattcc ggagtgcgcg agccgacgcg cgcgaacctg aacgctcgcg actttgcggt
      421 gatgcttgcg ggcgatcagg gctctgcgag ccccgcctgg tcgatcagga acgcgtattc
      481 ctccgccagc tcgcgcaggt gctcgaagcg gcccgacttg ccgccgtgtc ccgcctccat
      541 gttcacgcgc agcagcagca cgcgatcgtc cgtcctgaac cgacgcagcc tggcgaccca
      601 cttggccggc tcgtagtact gcacctggct gtcccacagc ccggtcgtga cgtacagcgc
      661 tggatacgca tgtgccgcga cgttgtcgta cggcgagtac gacagcatgt acgcgtagta
      721 ttcgcgctcg cccggattgc cccactcgtc gtattcattg gtcgtgagcg gaatgctctc
      781 gtcgagcatc gtcgtgacga cgtcgacgaa cggcacgtgg gcgaccatcc cgcggtacag
      841 ctcgggcgcc atgttggcga ccgccgccac cagcagtccg cctgcactgc cgccgcgcgc
      901 gaacacgcgc cccgggtcgg cgtagccctc ggcgacgagg aaccgcgtga catcgatgaa
      961 gtcctcgaaa ctgtggcgct tctcgagcag gcgtccctgc tcgtaccagc gtcgaccgag
     1021 ttcctgcccg ccgcggacct gtgcaatggc atagaggaag ccgcgatcga gcagcgagag
     1081 cagcgaggag gaaaacaaag gatccgacga aatgccgtag gcgccgtagc cgtactgcag
     1141 cagcggtgcg ctgccgttgc gctcgtagcg gtcgcggcga tacgcgatca ccaccggcac
     1201 ccgttcgccg tcgcgtgcca cggcccacag ctgctccgtc gcgtagcgtg acgggtcgaa
     1261 gtcgccgagg accggctcgc gcttgagcag cgtgcgctcg ccgttgcgca ggtcgtagtc
     1321 gtaggtggtg ggcggcgtcg tcagcgacgt gtacgtgtag cgcaccacct cgctgtcgat
     1381 ctcctcgttg gcgccgagcg ccatgcggta ggcggcctcg tccgagtcga ggtggaaatc
     1441 gcgcccgccg tgccacggcc ggatgcgcac cttgcgcagg ccctgcgtac gctcttcgag
     1501 tgcgagaaat ccagtgaaga cgtcgaagtc ggcgatcagc acgtcctcgc gatgcggtac
     1561 gacttcacgc caattcgcgc ggtcgccggc cgcgtcggtg gcagcctcaa ggatgcggaa
     1621 attgcgcgcc tgccagttgc tgcggatgat ccagcggtcg tcgacgtggt cgaccgtgta
     1681 ctcgtggccg cggtcccgcg gcagcagcac tttgaaatca aggcccaggt cgtccgcccg
     1741 tgcgtagcgc acctcgctgg tgatggtgct gctcgagtgg atcagcaggt agcgtcggtc
     1801 cttggtggtc tcgacgccca ggtagaacga gtcgtcgggt tcctcgtaga ccaggcggtc
     1861 ccg
//
LOCUS       DBNHBY010000787         9620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_399949, whole genome shotgun sequence.
ACCESSION   DBNHBY010000787 DBNHBY010000000
VERSION     DBNHBY010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_399949"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16HB_18455"
     CDS             <1..238
                     /locus_tag="AC16HB_18455"
                     /inference="COORDINATES: protein motif:HMM:NF023045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HIR5608829.1"
                     /translation="TESPQTYAISVGGLPGLKLASEREFEVPAASTRSVVLRLRAPGG
                     EVAPGSHKVAIDVQSQVQQDIAVRETTTFLGPRR"
     gene            235..771
                     /locus_tag="AC16HB_18460"
     CDS             235..771
                     /locus_tag="AC16HB_18460"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR5608830.1"
                     /translation="MNSATAKPQPWYREPWPWVLIALPAAAVIGCAVTLWLALASADG
                     MVARDYYRRGLAINSEITRTERAAQLGLAEEVDLDGFAGGDRVRVRLRAERALPVETA
                     LRLSFVHPGRDGADRTVVLGRVSGDAERAEFSGQFDAAAAMPAVAWQIVLESPSWRLD
                     GLMQGVNVRRVRLEAARR"
     gene            803..1144
                     /locus_tag="AC16HB_18465"
     CDS             803..1144
                     /locus_tag="AC16HB_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608831.1"
                     /translation="MTQSFVQRLLWVLWPAFLVAAVAELVLFAVFDPRDLYVFGVPVD
                     VDRMSAYTIGFFFLWAIASAASALTVFLQRSPFEINRCPLDATVRPPGCPKRADEACA
                     GCTPVPGNGRT"
     gene            complement(1141..1887)
                     /gene="fnr"
                     /locus_tag="AC16HB_18470"
     CDS             complement(1141..1887)
                     /gene="fnr"
                     /locus_tag="AC16HB_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR5608832.1"
                     /translation="MNAPAFSVTHLKVACSNCNLRELCLPLGLSLNDIERLEDMVATR
                     KRVRRGESLFRAGDRFESLYAVRLGFLKSTVMSTDGREQVTGFHMAGELVGMDGISKD
                     KHTCDTIALEDTEVCVIPYDRIEDVASSVPALRNHFHRVMSREIVREHGVMLLLGSMH
                     AEERLAAFLLNLSQRFESRGYSRSEFVLRMTRAEIGSFLGLKLETVSRVLSRFATEGL
                     IEVKQKHVRIVSPDGLRAIVSGQSGALSED"
     gene            2117..3517
                     /gene="hemN"
                     /locus_tag="AC16HB_18475"
     CDS             2117..3517
                     /gene="hemN"
                     /locus_tag="AC16HB_18475"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR5608833.1"
                     /translation="MLMSVRNGVALPDPALLRRFDIPGPRYTSYPTADRFVEAFDAQA
                     FESWLVRRGDTPSSAALSLYVHVPFCAQVCYYCACNKVITRDHTRGAEYLDALEREAE
                     MVSSKMSGGRQVEQLHFGGGTPTFLSNDEMKRLMAILTSRFPLAEKGEFSIEVDPRNA
                     APDKVAVLGELGFNRISVGVQDFDPAVQRAVNRLQSFEITQATIEAARKADFKSVNLD
                     LIYGLPKQSRETFARTLDKVLVLSPERIALYHYAHLPERFKPQRRIDAAELPSPQEKM
                     GIMLDAIQRLTEAGYRYIGMDHFAKASDDLARAQSQGRLHRNFQGYTTRPDCDLVGLG
                     VSAISKIGPTYSQNVRELDEYYERIRDGQLPTSRGVLLDMDDLVRRTVIMALMCHFEV
                     SKEAIETAHLIKFDDYFKRELAELKPFEEAGLVEVSPEWVSVTPKGKLLVRAVAMCFD
                     RYLRHDERIRKYSKIV"
     gene            3609..4352
                     /locus_tag="AC16HB_18480"
     CDS             3609..4352
                     /locus_tag="AC16HB_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5608834.1"
                     /translation="MVDLPLPQLLAAFVLSLLGGLHCAGMCGGFIGALQINRPRDVSP
                     LRLALGYHTGRIASYTAAGALVGALGGRLFAADVLPLQVLLLTLGSLMLLAIGSTLFG
                     RGAWLRRIEPLGAWIWRVVGPLARRVYPPRSGAQALVAGLAWGWIPCGMVYAALPLAL
                     VAGGPFAGALVMFAFGMGTLPNLALVDVAAVRLGSAGAGTLSASRAWLRPLAGAVIVV
                     FGISGLAHAARVAGADHPAIAALAAICHR"
     gene            complement(4357..5274)
                     /gene="menA"
                     /locus_tag="AC16HB_18485"
     CDS             complement(4357..5274)
                     /gene="menA"
                     /locus_tag="AC16HB_18485"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HIR5608835.1"
                     /translation="MSSFPAPTVRPGSLDAWAIAMRPRTLWLAAVPVLVGTALAWLES
                     GQVDWVVAILALAGSLLMQIITNLQNDVGYSARGHESQMRVGMPRATSSGLLTPREVR
                     AAIALAVACAIAVGLPLVLRGGWPVLAMGLASIVAALAYMGGPKPIAFTPLGELFVFA
                     FFGVIAVAGSGYVQIGAVTPATWIAACGVGLIAAAVLAVNNHRDIAHDARTGRHTFAV
                     TFGAQRSVRLYTALVLGAFVLLPLAALLAGHAALLLPLLALPLGWRLVRAVGEATAAA
                     MNQLLLGTVKLELIYGVLFTLGAVIAGLR"
     gene            5459..8110
                     /gene="alaS"
                     /locus_tag="AC16HB_18490"
     CDS             5459..8110
                     /gene="alaS"
                     /locus_tag="AC16HB_18490"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR5608836.1"
                     /translation="MKSAEIRDKFLKYFESKGHAIVRASSLVPANDPTLLFVNSGMVQ
                     FKDVFTGREKRPYSRATTAQKSLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIKYAWELLTQVYKLPPEKLWTTVYVDDDEAYDIWTREVGVPRDRCIRIGDKPGQP
                     KYVSDNFWQMADTGPCGPCSEIFYDHGPEVAGGPPGSPDADGDRYIEIWNLVFMQFDR
                     DEKGVLTPLPKPCVDTGMGLERLAAVLQHVHSNYEIDLFQNLIHAAARETQTTDLASN
                     SLKVIADHIRACAFLIADGVIPGNEGRGYVLRRIIRRALRHGYKLGRRQPFFHKLVRD
                     LVKEMGAAYPELAEQQARIEGVLKQEEERFGETLENGMKILDGALGGLKSGDARMLDG
                     ETVFLLYDTYGFPVDLTADICRERGISVDHAGFERAMERQREQARAAGKFRSAAGVEY
                     SGSRTRFIGYEHLTAEAKVVGLYVDGTPVSEVRAGEPGSERAIIVLDATPFYAESGGQ
                     VGDRGELIGSGGTFEVADTQKIMPDVFGHHGRVLSGSIEFGEKVTARVNVSLRTRTMH
                     NHSATHLMHGALRKVLGPHVQQKGSLVDAERTRFDFAHDKPMTVDEIRHVEDIVNAAI
                     RANAPVTATHMKYDDAIRAGALAFFGDKYGDEVRVLAMGEHSTELCGGTHVSRTGDIG
                     FFKIVAEGGVAAGVRRVEAVTGAGALAFVQALDAQLADIAATLKAPAAEVSAKLAHVQ
                     DHVKALEKELARLKGKLAASQGDDLASRAIDIKGIKVLAATLEGADARVLRDTVDQLK
                     NKLKSAAIVLAAVDGKEGETRVQLAAGVTADVTAKLKAGDLVNHVAQQIGGRGGGRAD
                     MAMAGGTDAAALPKALASVKDWAEKRL"
     gene            8122..8646
                     /locus_tag="AC16HB_18495"
     CDS             8122..8646
                     /locus_tag="AC16HB_18495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5608837.1"
                     /translation="MTPSSIRIRREEPEDADALARVLGDEGVYPQTLQLPHVSRHFWR
                     ERLAKYDPAKYALVAEIDGSIVGSAGLFINERPRQRHCAMLGIGVAADHSGRGVGSAL
                     MRDLLRAADGWLNVLRVELTVFVDNERAIRLYQRFGFAIEGTLRAHALRNGVYVDGYV
                     MGRLHPHPPQLSRA"
     gene            8643..9155
                     /locus_tag="AC16HB_18500"
     CDS             8643..9155
                     /locus_tag="AC16HB_18500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5608838.1"
                     /translation="MTDTGYRFCPACATPLTRAICGERERSACPACGWVHWDNPVPVV
                     AAVIEYEGRVLLARNAAWPAGSFALVTGFLERDETPRDAVIREVKEETNLDALDAQLI
                     GVYDFALRNQVIIAYHVSAAGDVKLNEELVEYRLIEPARVKTWPRGTGFALADWLRTR
                     GHEVHFVPLP"
     gene            9170..9409
                     /locus_tag="AC16HB_18505"
     CDS             9170..9409
                     /locus_tag="AC16HB_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5608839.1"
                     /translation="MQTTIVFDKEIDTRGLNCPLPVLRAKKALAELASGQVLKIVATD
                     PGSIRDFKAFAKQTGNELLAQETVNDEFIHFLRRR"
     gene            9412..>9620
                     /locus_tag="AC16HB_18510"
     CDS             9412..>9620
                     /locus_tag="AC16HB_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608840.1"
                     /translation="MKLAITVVLGLIVLGAAWLWFALNWNYSAGERAGWVQKFSKKGW
                     VCKTWEGELAMVSMPGAAQEKFYFTV"
BASE COUNT         1586 a         3198 c         3333 g         1503 t
ORIGIN      
        1 gaccgagtcg ccgcagacct acgcgatctc ggtcggcgga ctgcccggat tgaagctcgc
       61 atccgaacgg gagttcgaag tgccggcggc gagcacccgt tcggtggtat tgcggctgcg
      121 cgcgccgggc ggcgaggtgg cgccgggatc gcacaaggtc gcgatcgacg tgcagtcgca
      181 ggtgcagcag gacatcgcgg tccgcgagac gacgacgttt ctggggccgc gccgatgaac
      241 tccgccaccg ccaagcccca gccctggtat cgcgagccgt ggccgtgggt cctgattgcg
      301 ctgcccgccg cggcggtcat cgggtgcgcg gtcacgctat ggctcgcgct cgccagcgcc
      361 gacggcatgg tcgcgcgcga ctattaccgg cgcgggctgg caatcaatag tgaaatcaca
      421 cggaccgagc gcgcggcgca gctcggtctg gcggaggagg tggatctcga cggcttcgcc
      481 ggcggcgatc gcgtgcgcgt gcgcctgcgc gccgagcgcg cgctgccggt cgaaacggcg
      541 ctgcggctgt cgttcgtgca tcccgggcgc gacggcgccg atcgcaccgt ggtccttggg
      601 cgggtgagcg gcgacgccga gcgcgccgag ttcagcggcc aattcgacgc cgcggcagcg
      661 atgcccgcgg tcgcgtggca gatcgtgctt gaatcgccaa gctggcggct cgatgggctg
      721 atgcagggtg tgaacgtacg ccgcgtccgt ctcgaggccg cgcggcgatg agggcaggat
      781 actgaccgca ggggaggcga ggatgacgca aagcttcgtg cagcggctgc tctgggtgct
      841 atggccggcg tttctggtcg ctgcggtcgc ggaactcgtg ctgttcgcgg tattcgaccc
      901 gcgcgatctg tacgtcttcg gcgtgcccgt cgacgtcgat cgaatgtccg cctacacgat
      961 cggcttcttc tttctgtggg cgatcgcgtc ggcggccagc gcgctcacgg ttttcctgca
     1021 gcgctcgccg ttcgagatca atcgctgccc gctcgacgca accgtgcgcc cgccgggctg
     1081 cccgaaacgg gccgacgaag cctgcgccgg ctgtacgccg gtgcctggaa acggacgtac
     1141 ctagtcttcg ctgagtgcgc ccgactggcc gctgacgatc gcgcgcagcc cgtccggcga
     1201 gacgatgcgc acgtgcttct gcttcacctc gatcagcccc tcggtggcga agcgcgacag
     1261 cacgcggctg acggtctcca gcttcaggcc gaggaagctg ccgatctcgg cgcgcgtcat
     1321 ccgcagcacg aactcggagc gcgaatagcc gcgcgattcg aagcgctgcg agaggttcag
     1381 caggaacgcc gccaggcgct cttccgcgtg catgcttccc agcagcagca tcacgccgtg
     1441 ctcgcgcacg atctcgcggc tcatcacgcg gtggaagtgg ttgcgcagcg ccggcaccga
     1501 cgaggcgacg tcctcgatgc ggtcgtacgg gatgacgcac acctcggtat cctcgagcgc
     1561 gatcgtgtcg caggtgtgct tgtctttgct gatgccgtcc atgccgacca gttcgccggc
     1621 catgtgaaag ccggtgacct gctcgcggcc atcggtcgac atcacggtac tcttcaggaa
     1681 gcccaggcgc accgcgtaca gcgattcgaa gcggtcgccg gcgcggaaca ggctttcgcc
     1741 gcggcgcacg cgcttgcgcg tggcaaccat atcctccagc cgctcgatgt cgttcaacga
     1801 cagaccgagc ggcagacaca gttcacgcag gttgcagttc gagcaggcaa ccttcaggtg
     1861 cgtgacggaa aaggcaggcg cattcatctc gatcgatccc aagtcgactg ttcgactgtc
     1921 tcgctgccag cgtattcccg cagctgctgt ccgggttttc cttgcggcgt cgcgctggcg
     1981 gcatcgcacg cgcctctgtg ctcggaccgc gttgatccgt gtcaacgccc ccctgcgagc
     2041 ggtccgaata atacccttcg agccaactgc ggttgcttaa actgcgggat tgagtgtaac
     2101 gggaaagggt acacggatgc tgatgtctgt tcgcaatggc gtggcgctgc cggatcctgc
     2161 cctgttgcgc cggttcgata tccccggtcc gcgctacacg tcgtatccga ccgccgatcg
     2221 cttcgtcgag gcgtttgacg cgcaggcgtt cgaaagctgg ctggtgcggc gcggcgatac
     2281 ccccagttcg gcggcgctgt cgctttacgt gcatgtgccc ttttgcgcgc aggtctgcta
     2341 ctactgcgca tgcaacaagg tgatcacgcg cgatcacacg cgcggcgccg agtacctcga
     2401 cgcactcgag cgcgaggccg agatggtcag cagcaagatg tccggcggcc gccaggtcga
     2461 gcaactgcac tttggcggcg gcacgccgac ctttctcagc aacgacgaaa tgaagcgcct
     2521 gatggcgatc ctgacgtcgc gctttccact ggcggagaag ggcgagttca gcatcgaagt
     2581 cgatccgcgc aacgcggcgc cggacaaggt cgccgtgctc ggcgagctcg gtttcaaccg
     2641 catttcggtc ggcgtgcagg atttcgaccc ggccgtgcag cgcgccgtga accggctgca
     2701 gagcttcgag atcacgcagg cgacgatcga agccgcgcgc aaggccgact tcaagtcggt
     2761 gaacctcgac ctcatctacg gcctgccgaa gcagtcgcgc gagaccttcg ccaggacgct
     2821 cgacaaggtg ctggtgctca gccccgagcg catcgcgctg tatcactacg cgcacctgcc
     2881 ggagcgcttc aagccacagc gccggatcga tgccgcggag ctgccgtcgc cgcaggagaa
     2941 gatgggcatc atgctcgacg cgatccagcg cctgaccgag gccggctatc gctacatcgg
     3001 catggaccac ttcgccaagg cgtccgacga tctcgccagg gcgcaaagcc agggccggct
     3061 gcaccgcaac ttccagggtt acacgacgcg gcccgactgc gacctggtcg gcctgggcgt
     3121 ctcggcgatc agcaagatcg gcccgacgta ttcgcagaac gtgcgcgagc tcgacgagta
     3181 ctacgagcgc atccgcgacg gccagctgcc gacctcgcgc ggcgtgctgc tcgacatgga
     3241 cgacctggtg cggcgcacgg tgatcatggc gctgatgtgc cactttgaag tgtcgaagga
     3301 ggcgatcgaa accgcgcacc tgatcaagtt cgacgactac ttcaagcgcg agctggcgga
     3361 actgaagccg ttcgaggaag ccgggctggt cgaagtgtcg ccggagtggg tcagcgtgac
     3421 gccgaagggc aagctcctgg tgcgcgcggt ggcgatgtgc ttcgaccgct acctgcgaca
     3481 cgacgagcgc atccggaaat actcgaagat cgtctgacgc ggtcgtatcg agccgcatcg
     3541 gactcacctg gttcgagcgg tcgctaggcg cgctgctcga tttggcgact cacggcacgc
     3601 acttgctcat ggtcgatctg ccgcttcccc agttgctggc ggcgttcgtg ctgtcgctgc
     3661 tcggcgggct gcactgcgcg ggcatgtgcg gcggcttcat cggtgcgctg cagatcaatc
     3721 gtccgcgcga cgtttcgccg ctgcggctgg cgctcggcta tcacacggga cggatcgcca
     3781 gctacacggc ggcgggcgca ctggtcggcg cgcttggcgg tcggctcttc gcggccgacg
     3841 tgttgccgct gcaggtgctg ctgctcacgc tcggcagcct gatgctgctg gcgatcggct
     3901 cgacgctgtt cggccgcggc gcgtggctcc ggcgcatcga gccgctcggg gcgtggatct
     3961 ggcgcgtagt cgggccgctg gcgcggcgcg tgtacccgcc ccgctcgggt gcacaggcac
     4021 tggtggccgg actcgcgtgg ggctggattc cgtgcggcat ggtgtacgcg gcgctgccgc
     4081 tcgcgctcgt cgccggcgga ccgttcgccg gtgcgctggt gatgttcgcg ttcggaatgg
     4141 ggacactgcc gaacctggcg ctggtcgacg tcgctgccgt caggctcggc agcgcgggcg
     4201 ccggcacgct ctcggcgtcg cgcgcctggc tgcggccgct cgccggcgcg gtgatcgtcg
     4261 tgttcggcat atccgggctc gcgcacgcgg cgcgcgtggc gggcgccgat catccggcga
     4321 tcgccgcctt ggcggcgatc tgccatcggt gacgcgtcag cgcagtcctg caatcacggc
     4381 gccgagcgtg aacagcacgc cgtagatcag ttccagcttc accgttccga gcagcaactg
     4441 attcatcgcc gcggccgtcg cctcgcccac cgcgcgcacc agccgccagc cgaggggcag
     4501 cgccagcagc ggcagcagca gtgcggcgtg tcccgcgagc agcgcagcca ggggcaagag
     4561 aacaaatgcg ccgagcacga gcgccgtgta caacctgacc gagcgttgcg cgccgaacgt
     4621 caccgcaaag gtgtgccggc cggtgcgcgc atcgtgggcg atgtcgcggt ggttgtttac
     4681 cgccagcacc gcggccgcga tgagtccgac gccacacgcg gcgatccagg tcgccggcgt
     4741 cacggcgccg atctgcacat agccgctgcc cgccaccgcg atcacgccga agaatgcgaa
     4801 cacgaacaac tcgcccagcg gcgtgaaggc gatcggcttc ggcccaccca tatacgcgag
     4861 cgcggcgacg atcgacgcca gccccatcgc cagcaccggc cagccgccgc gcagcaccag
     4921 cggcaagccg accgcgattg cgcacgcgac ggccagcgcg atggcggcgc gcacttcacg
     4981 cggcgtcagc agcccgctcg atgtcgcgcg cggcatgccg acccgcatct ggctctcatg
     5041 cccgcgtgcg ctgtagccga cgtcgttctg caggttggtg atgatctgca tcagcagcga
     5101 cccggcgagc gccagtatcg cgacgaccca gtcgacctgc ccgctttcga gccatgcgag
     5161 cgcggtgccg accagcaccg gcaccgcggc gagccacagc gtgcgcggcc gcatcgcgat
     5221 cgcccatgcg tcgagcgagc cggggcgcac cgtcggggcg gggaaggagg acatcaaagc
     5281 gttcatggtt gccttcagga cgcgaagata gcgaacacag cggcaatttc aacccgtcga
     5341 gcccggtgca ccgttgatct cgatcgagga acggcagggt cgcctgatat catcgattcg
     5401 agtgaaaaat cctgtcgcga cgcgtagttg cgcgtcgcgc aagccgctgc ccgatactat
     5461 gaaaagcgcc gagatccgcg acaagttcct caagtacttc gaatccaagg gccacgcgat
     5521 cgtgcgggcc tcgtcgctgg tgccggccaa cgatccgacg ctgctgttcg tcaacagcgg
     5581 catggtgcaa ttcaaggacg tgttcacggg ccgggagaag cgcccgtact cgcgcgcgac
     5641 caccgcgcag aagtcgctgc gtgccggcgg caagcacaac gacctggaga acgtcggcta
     5701 caccgcgcgc caccacacgt tcttcgagat gctgggcaac ttcagcttcg gcgactactt
     5761 caagcgcgac gcgatcaagt atgcgtggga actgctgacc caggtctaca agctgccgcc
     5821 ggagaagctg tggaccaccg tctacgtcga cgacgacgag gcgtacgaca tctggacgcg
     5881 cgaggtcggc gtacccaggg atcgctgcat ccgcatcggc gacaagccgg ggcagccgaa
     5941 gtacgtctcg gacaacttct ggcagatggc ggacaccggc ccgtgcgggc cgtgcagcga
     6001 gatcttctac gaccacggac ccgaggtcgc cggcgggccg cccggatcgc ccgacgccga
     6061 cggcgaccgc tacatcgaaa tctggaacct cgtgttcatg cagttcgacc gcgacgagaa
     6121 gggcgtgctg acgccgctgc cgaagccgtg cgtcgacacc ggcatgggac tggagcggct
     6181 ggcggcggtg ctgcagcacg tgcacagcaa ctacgagatc gacctgttcc agaacctgat
     6241 ccatgcggcg gcgcgcgaga cccagacgac cgacctcgcc agcaattcgc tgaaggtgat
     6301 cgccgaccac attcgcgcct gtgcgttcct gatcgccgac ggcgtgattc ccggcaacga
     6361 agggcgcggc tacgtgctgc gccggatcat ccgccgtgcg ctgcgccacg gctacaagct
     6421 gggccgccgg cagccgttct tccacaagct ggtgcgcgac ctggtgaagg aaatgggcgc
     6481 cgcctatccg gaactcgccg agcagcaggc gcgcatcgag ggcgtgctca agcaggagga
     6541 agagcgcttc ggcgaaacac tggaaaacgg catgaagatc ctcgacggcg cgctcggcgg
     6601 gctgaagagc ggcgacgcgc gcatgctgga cggcgagacc gtgttcctgc tgtacgacac
     6661 gtacggcttc ccggtggacc tgaccgccga catctgccgc gagcgcggca tcagcgtcga
     6721 ccacgccggc ttcgagcgcg cgatggagcg gcagcgcgag caggcgcgcg cggcgggcaa
     6781 gttccgcagc gcggccggcg tcgagtacag cggcagccgc acgcgcttca tcggctacga
     6841 gcacctgacc gccgaggcga aggtcgtggg cctgtacgtc gacggcacgc ccgtcagcga
     6901 agtgcgcgcc ggtgagccgg gatcagagcg cgcgatcatc gtgctcgatg ccacgccgtt
     6961 ctatgcggaa agcggcggcc aggtcggcga tcgcggcgaa ctgatcggca gcggcggcac
     7021 gttcgaggtc gccgacacgc agaagatcat gccggacgtg ttcggccacc acggcagggt
     7081 gctgtcgggc tcgatcgagt tcggcgagaa agtgaccgcg cgcgtgaacg tgtcgctgcg
     7141 cacgcgcacg atgcacaacc actcggcgac gcacctgatg cacggcgcgc tgcgcaaggt
     7201 gctcggcccg cacgtgcagc agaaaggctc gctggtcgac gccgagcgca cgcgcttcga
     7261 cttcgcgcac gacaagccga tgaccgttga cgagatccgc cacgtcgagg acatcgtcaa
     7321 tgcggcgatc cgcgccaatg cgccggtgac cgccacgcac atgaagtacg acgacgcgat
     7381 ccgcgccggc gcgttggcgt tcttcggcga caagtacggc gacgaggtgc gcgtgctggc
     7441 gatgggcgag cactcgacgg agctgtgcgg tggcacgcat gtttcccgta ccggcgacat
     7501 cggcttcttc aagatcgttg ccgagggcgg cgtggcggcc ggcgtgcgac gcgtcgaggc
     7561 ggtgacagga gccggcgcgc tcgcgttcgt gcaggcgctc gacgcgcagc tggccgacat
     7621 cgcggcgacc ctgaaggcgc cggcggccga ggtgtccgcc aagctcgcgc acgtgcagga
     7681 ccacgtgaag gcgctcgaga aggaacttgc gcgcctgaag ggcaagctcg ccgccagcca
     7741 gggcgacgat ctcgcttccc gcgcgatcga catcaagggc atcaaggtgc tcgccgccac
     7801 gctcgaaggc gccgacgcca gggtgctgcg cgacactgtc gaccagctga agaacaagct
     7861 gaagtccgct gccatcgtcc tggcggccgt cgacggcaag gaaggagaaa cgcgggtgca
     7921 gctcgccgcc ggcgtgaccg ccgatgtcac agcaaagctg aaggcgggcg acctcgtcaa
     7981 ccacgtcgcg cagcagatcg ggggccgggg cggcggccgc gccgacatgg cgatggccgg
     8041 cggcaccgat gcggccgcgt tgccgaaggc gctcgccagc gtgaaggact gggccgagaa
     8101 gcgcttgtag cgcacgaacc gatgacgccg tcctcgatcc gcattcgccg cgaggagccg
     8161 gaggacgccg acgcgctggc gcgggtgctc ggcgacgagg gcgtgtaccc gcagacgctg
     8221 cagcttcccc atgtgtcacg ccatttctgg cgcgagcggt tggcgaaata cgacccggcc
     8281 aaatatgcgc tcgtggccga aatcgacggc agcatcgtcg ggtcggcggg gttgttcatc
     8341 aatgagcgtc cgcgccagcg ccattgcgcg atgctcggca tcggcgtcgc agcggatcac
     8401 tcgggccgtg gcgtgggctc ggcgctgatg cgcgacctgc tgcgcgcggc cgacggctgg
     8461 ctcaacgtgc tgcgcgtgga actgacggtc tttgtcgaca acgagcgcgc gatccggctt
     8521 taccagcgct tcggttttgc catcgagggc acgctgcgcg cccatgcgct gcgcaatggt
     8581 gtctatgtcg acggctacgt gatgggccgc ctgcatcccc atccgccgca actgtcgcgc
     8641 gcgtgaccga cacgggctat cgcttctgcc ccgcgtgcgc cacgccgctc acgcgcgcca
     8701 tctgtggtga gcgcgagcgc agcgcctgcc cggcgtgcgg ctgggtgcac tgggacaacc
     8761 ccgttcccgt ggtcgccgcg gtgatcgaat acgagggccg ggtgctgctg gcgcgcaatg
     8821 ccgcgtggcc cgcgggtagc ttcgccctcg tcaccgggtt tctcgagcgc gacgagacgc
     8881 cgcgcgacgc ggtcatccgc gaagtgaagg aggagaccaa cctcgacgcg ctcgacgcgc
     8941 aactgatcgg cgtgtacgac tttgcgctgc gcaaccaggt catcatcgcc tatcacgtgt
     9001 cggccgccgg cgacgtgaag ctgaacgagg agctcgtcga gtaccggctg atcgagccgg
     9061 cgcgcgtgaa gacctggccg cgcggcacgg gcttcgcgct cgccgactgg ctgcgcacgc
     9121 ggggtcacga agtacacttc gtgccgctgc cgtagcgagt gaacgaacca tgcagactac
     9181 gatcgtgttc gacaaagaga tcgacacgcg cgggctgaac tgcccgctgc cggtgctgcg
     9241 cgcgaagaag gcgctggcgg agctggcgag cggccaggtg ctgaagatcg tcgccaccga
     9301 tcccggctcg atccgcgact tcaaggcgtt tgccaagcag accggcaacg agctgctggc
     9361 gcaggaaacg gtgaacgacg aattcatcca cttcctgcgg cggcgctgac agtgaagctc
     9421 gccatcaccg tcgtcctcgg cctgatcgtg ctgggcgccg cctggctctg gttcgcgctg
     9481 aactggaact actcggccgg cgagcgcgcg ggctgggtgc agaagttcag caagaagggc
     9541 tgggtgtgca agacctggga aggcgagctg gcgatggtat cgatgcccgg cgccgcgcag
     9601 gagaagttct acttcaccgt
//
LOCUS       DBNHBY010000788         1914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_129654, whole genome shotgun sequence.
ACCESSION   DBNHBY010000788 DBNHBY010000000
VERSION     DBNHBY010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_129654"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1914
                     /locus_tag="AC16HB_18515"
     CDS             <1..>1914
                     /locus_tag="AC16HB_18515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5608841.1"
                     /translation="RATIFSGAGVVTRRVADAAERAGLVFAVDPTSADSSCIGGNIAM
                     NAGGKKAVLWGTALDNLVWWRMVDPEGNWLEVTRVEHNLGKIHDAPVAKFDLVWKDGR
                     QSPARAKELKREHLEIEGRRFRKTGLGKDVTDKFLGGLPGIQKEGCDGLITSARWLLH
                     RMPQHTRTVCLEFFGNARDAVPSIVEIKNLLAAKPGGVLLAGLEHLDERYLRAVGYTT
                     KSRRGALPKMVLIGDLVGDDDAAVAKAASAVVRIANARAGEGFVAVNSEARKKFWLDR
                     AKTAAIARHTNAFKLNEDVVIPLERMGDYTNAIERINIELSIRNKLELLDEIDAYLSG
                     AIHLGKTGDVEFDRLPRQEILGDRIEQARELIAYVRERWRYLLQHFDTPLTQAAPQLA
                     ALGVTHPAAPDRAPADVIDLLQERTIRVSWKTEVRAPLERIFGGSAFAPVLADCEAIH
                     QRVLHGRVFVALHMHAGDGNVHTNIPVNSDNYEMLRTAHATVARIMRIARDLDGVISG
                     EHGIGITKLEFLREDETREFRAYKERIDPEGRFNKGKLLPGATLDNAYTPSFNLLGHE
                     SLIMQQSDINSIADAIKDCLRCGKCKPVCATHAPRANLLYSPRNKILATSLLIEAFLY
                     EEQTRRGVSIKHW"
BASE COUNT          335 a          669 c          647 g          263 t
ORIGIN      
        1 gcgcgcgacg atcttctccg gcgcgggcgt ggtcacgcgc cgcgtcgccg acgccgccga
       61 acgcgccggg ctcgtgttcg cggtcgatcc gacctcggcc gactcgtcgt gcatcggcgg
      121 caacatcgcg atgaacgccg gcggcaagaa ggcggttctg tgggggaccg cgctcgacaa
      181 cctcgtgtgg tggcgcatgg tcgaccccga gggcaactgg ctcgaggtca ctcgcgtcga
      241 gcacaacctg ggcaagattc acgatgcgcc ggtcgccaag ttcgacctcg tctggaagga
      301 cggccgccag agccccgcgc gcgcgaagga gttgaagcgc gagcacctcg agatcgaggg
      361 ccggcgcttc cgcaagacgg ggctcggcaa ggacgtcacg gacaagttcc tcggcggcct
      421 gcccggcatc cagaaggaag gctgcgacgg cctgatcacg tcggcgcgct ggctgctgca
      481 ccggatgccg cagcacacgc gcaccgtgtg cctggagttc ttcggcaacg cgcgcgacgc
      541 ggtgccgtcg atcgtcgaga tcaagaacct gctcgccgcc aagcccggcg gcgtgctgct
      601 cgcgggtctg gagcatctgg acgagcgcta cctgcgcgcg gtcggctaca cgaccaagtc
      661 caggcgcggc gcgctgccga agatggtgct gatcggcgat ctcgtcggcg acgacgacgc
      721 cgcggtggcc aaggcggcga gcgcggtcgt gcgcatcgcc aacgcgcgcg ccggcgaggg
      781 cttcgtcgcg gtcaacagcg aggcgcgcaa gaagttctgg ctcgaccgcg ccaagaccgc
      841 cgcgatcgcg cgccacacca acgcgttcaa gctgaacgag gacgtggtga tcccgctcga
      901 gcgcatgggc gactacacca atgcgatcga gcgcatcaac atcgagctgt cgatccgcaa
      961 caagctcgaa ctgctggacg agatcgacgc gtatctctcc ggggcgatcc acctcggcaa
     1021 gaccggcgac gtcgagttcg accgcctgcc ccgccaggag atcctcggcg accgcatcga
     1081 acaggcgcgc gagttgatcg cctacgtgcg cgagcgctgg cggtacctgc tgcagcactt
     1141 cgatacgccc ctcacgcagg cggcgccgca actggccgcg ctgggcgtca cgcacccggc
     1201 tgcacccgac cgcgcgccgg ccgacgtgat cgacctcctg caggagcgca cgatccgcgt
     1261 gtcgtggaag accgaggtac gcgcgccgct ggagcggatc ttcggcggca gcgcgttcgc
     1321 cccggtgctg gccgactgcg aggcgattca ccagcgcgtg ctgcacgggc gcgtgttcgt
     1381 ggcgctgcac atgcacgccg gcgacggcaa cgtgcacacc aacatcccgg tcaactccga
     1441 caactacgag atgctgcgca ccgcgcacgc caccgtcgcg cgcatcatgc ggatcgcgcg
     1501 cgacctggat ggcgtgatct ccggcgagca cggcatcggc atcaccaagc tcgaattcct
     1561 gcgcgaggac gagacgcgcg agttccgcgc gtacaaggag cgcatcgacc ccgagggccg
     1621 cttcaacaag gggaagctgc tgcccggcgc cacgctcgac aacgcgtaca cgccgagctt
     1681 caacctgctc ggccacgagt cgctgatcat gcagcagtcc gacatcaatt cgatcgcgga
     1741 tgcgatcaag gactgcctgc ggtgcggcaa gtgcaagccg gtgtgcgcca cccacgcgcc
     1801 gcgcgccaac ctgctctatt cgccgcgcaa caagatcctg gccacgtcgc tgctgatcga
     1861 ggcgttcctg tacgaagagc agacgcggcg cggcgtcagc atcaagcact ggga
//
LOCUS       DBNHBY010000789         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1889106, whole genome shotgun sequence.
ACCESSION   DBNHBY010000789 DBNHBY010000000
VERSION     DBNHBY010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1889106"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="AC16HB_18520"
     CDS             complement(<1..1238)
                     /locus_tag="AC16HB_18520"
                     /inference="COORDINATES: protein motif:HMM:NF025665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase WbsX family protein"
                     /protein_id="HIR5608842.1"
                     /translation="MTDGQHPATADVRLIAFYLPQFHPTEENDRWWGKGFTEWTNVTR
                     AQPLFEGHYQPHLPTELGFYDLRLRASRHEQIALTREHGVDAFCYHYYWFSGRRLLRQ
                     PLDDMLVDPASEMPFCLCWANETWTRRWDAADHEVLIAQQYLPGDDLQFIESVGPFLR
                     DPRYLRVSGRPLLIVYRPHEIPNPAAWADTWRRHCRATGIGEIHLCGAFTHGHRSVAG
                     LGYDSGVEFPPHNAWATSRNPQIRFHRPFSGNVLDYADVAQGFLANRYADDTVFRTVV
                     PSWDNTARTDARANVLVNATPANYEAWLRGAVERTARERRTGERLVFINAWNECAEGC
                     HLEPDRRHGRGYLEATLRVRRGASTATSFVDRFADRPTAAPPRGFGQDLVELLRYHLL
                     SRIGPLRVWMNRHPRVRAAAL"
     gene            complement(1258..2103)
                     /gene="galU"
                     /locus_tag="AC16HB_18525"
     CDS             complement(1258..2103)
                     /gene="galU"
                     /locus_tag="AC16HB_18525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR5608843.1"
                     /translation="MKVRKAVFPAAGFGTRFLPATKAMPKEMMPVVDKPLIQYAVEEA
                     AAAGIEQMIIITGRNKRAIEDHFDRIPELEQELLAKGKRELYEALHSATPSGVSFIFV
                     RQSEQLGLGHAVLCAQPVVGNEPFAVILADDLIDAQPGVTQQLVATYEKYNASVLAVQ
                     DVPRAETSRYGIVAASPIAPGIERVSGMVEKPKPAEAPSTLAVVGRYVLRPEIFAELA
                     STPRGSGGEIQLTDAIARRIPHGEVLAQRFSGTRFDCGNKEGFLAANIALARKRGILL
                     DGRAD"
     gene            complement(2124..2273)
                     /locus_tag="AC16HB_18530"
                     /pseudo
     CDS             complement(2124..2273)
                     /locus_tag="AC16HB_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          364 a          758 c          767 g          384 t
ORIGIN      
        1 agcgcggcag cgcggacgcg cggatgccga ttcatccaga cacgcagggg accgatgcgc
       61 gacagcaggt ggtatcgcag cagctcaacg agatcctggc cgaaaccgcg tggcggcgcc
      121 gctgtcggcc ggtcggcgaa tcggtcgacg aagctcgtgg cagtgctcgc cccgcgccgc
      181 acgcgaagcg tcgcctcgag atagccgcgg ccgtggcggc gatccggctc caggtgacag
      241 ccttcagcac attcgttcca ggcattgatg aacaccaacc gttccccggt gcgtcgttcg
      301 cgcgcggttc gctcgaccgc cccgcgcagc caggcctcgt agttggccgg ggtcgcattc
      361 accagcacgt tcgcgcgcgc gtccgtgcgc gcggtgttgt cccacgacgg caccacggtg
      421 cggaaaaccg tgtcatccgc atagcgattg gccagaaatc cctgcgcgac gtcggcgtag
      481 tcgagaacgt tgcccgaaaa cggcctgtgg aaccggatct gcggattcct gctggtggcc
      541 caggcgttat gcggcggaaa ctcgacgccg ctgtcgtagc cgagcccggc gaccgatcga
      601 tgaccatgcg tgaatgcacc gcagagatgg atctcgccga tgcctgtggc ccggcaatgg
      661 cggcgccacg tatccgccca tgccgccggg ttcggaatct cgtgcgggcg atagacgatc
      721 agtagcggcc ggccgctcac gcgcaggtag cgtggatcgc gcaggaaggg tccgacgctt
      781 tcgatgaact gcagatcgtc gccgggcagg tactgctgcg cgatcagcac ctcgtgatcg
      841 gctgcgtccc agcggcgcgt ccaggtttcg ttggcccagc acaggcagaa cggcatctcg
      901 ctggctggat cgacgagcat gtcgtcgagc ggctggcgca gcaggcgccg ccccgaaaac
      961 cagtagtagt ggtagcagaa cgcgtcgacg ccgtgctcgc gcgtcagcgc aatctgctca
     1021 tgccgcgacg cgcgcagtcg caggtcgtag aagccgagct cggtcggcag atgcggctgg
     1081 tagtgaccct cgaacagcgg ctgcgccctg gtgacgttgg tccactcggt gaaccccttg
     1141 ccccaccagc gatcgttttc ctcggtcgga tgaaactgcg gcaggtaaaa ggcgatcagg
     1201 cgaacgtccg cggtggctgg gtgctgaccg tccgtcatgc gcacttcgcc gcgggcgtca
     1261 gtcggcgcgc ccatccagca ggatgcctcg cttgcgcgcc agcgcaatgt tggcggccag
     1321 aaagccctcc ttgttgccgc agtcgaagcg cgtgccgctg aaccgctgcg ccagcacctc
     1381 gccgtgcggg atgcggcgtg cgatcgcgtc ggtcagctgg atctcgccgc cgctgccgcg
     1441 tggcgtggat gccagttcgg cgaagatctc cggccgcagc acgtagcgcc cgaccacagc
     1501 gagcgtggat ggcgcctcgg caggcttggg cttctccacc atgccgctga cacgctcgat
     1561 gcccggcgcg atcgggctgg ccgcgacgat gccgtagcgc gatgtctccg cgcgcggcac
     1621 atcctgcacc gcgagcaccg acgcgttgta cttctcgtag gtcgcgacga gctgctgcgt
     1681 cacgcccggc tgcgcgtcga tcaggtcgtc ggcgagaatc accgcgaacg gctcgttgcc
     1741 gaccaccggc tgtgcgcaca gcaccgcgtg accgaggccg agctgctcgc tctgccgcac
     1801 gaagatgaag ctgacgccgc tgggtgtggc cgaatgcagc gcctcgtaca gttcgcgctt
     1861 gcccttggcc agcagctcct gttcgagttc cgggattctg tcgaagtgat cctcgatcgc
     1921 gcgtttgttg cggccggtga tgatgatcat ctgctcgata ccggcggcgg ccgcttcctc
     1981 gaccgcgtac tggatcagcg gcttgtcgac caccggcatc atctccttcg gcatcgcctt
     2041 cgtggccggc aggaaacgcg tgccgaagcc ggccgcgggg aacaccgctt tgcgaacctt
     2101 cattgggtca accgttgcgt cagaagttgc ttgccgatct cgaccccgaa ctggtcgaag
     2161 gcgttgatgt tccacagtac gctctgcacg aacgtgcggt gctcgaacaa cgcgagcagc
     2221 gcgccgagcg cgcgggcgtc gagctgcggc acggtgatga acgaactcgg ccg
//
LOCUS       DBNHBY010000790         2291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_266231, whole genome shotgun sequence.
ACCESSION   DBNHBY010000790 DBNHBY010000000
VERSION     DBNHBY010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_266231"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..839)
                     /locus_tag="AC16HB_18535"
     CDS             complement(3..839)
                     /locus_tag="AC16HB_18535"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR5608844.1"
                     /translation="MSPDQFFVLAAILVGLVVGSFLNVVIHRVPRMMEADWRAQCAEL
                     DGKAPAESGPYNLFVPASHCPACKTPLRIRDNVPLISYLHLRGRCAACGAPISPRYPI
                     VEGLTAALSAWTAWHFGVGSQGLLALFFVWALIALTFIDADTTLLPDSVTLPLLWLGL
                     LANTIDTFAPLRDAVIGAVAGYLILWSIYWLFKLLTGKEGMGHGDFKLLAALGAWFGW
                     KMLLPIVLLSSVVGTMVGIVLMILARRGRDIPIPFGPYLAAAGFIALLYGRELVTRFV
                     PV"
     gene            complement(886..2115)
                     /locus_tag="AC16HB_18540"
     CDS             complement(886..2115)
                     /locus_tag="AC16HB_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5608845.1"
                     /translation="MATGALIRPRAPARETLFTWEGRDKNGKVVRGEMRAGGESVVAA
                     SLRRRGVLATKIKKQTFSRGRRISDKDLAIFTRQLATMLRAGVPLMQCFDIVARGHGN
                     PSMARLLNDIRADVETGTSLNQAFRKYPLYFDPLFCNLVAAGEQAGILETLLDRLATY
                     KEKTLALKGKIKKAMFYPIMVILVAILITAIIMYFVIPSFKQVFRSFGADLPAPTLAV
                     IAISDFVVDYWYVLLAFLIGLPYFVFQAWKRSPNFQILVDRLMLKPPVVGELLQKASV
                     ARWCRTLSTTFAAGVPLVEALDSVGPASGNAVYREATKQIQTEVNIGTSLAQAMQNSA
                     VFPSMAVQMTSIGEESGSLDSMLSKVADYYEREVDEMVDAISSLIEPAIMVVLGVLVG
                     GIVIAIYLPIFKLGQVI"
BASE COUNT          461 a          723 c          720 g          387 t
ORIGIN      
        1 cgtcagaccg ggacgaaccg tgtgacgagt tcgcgaccat agagcagcgc aatgaatcca
       61 gctgcggcaa gataggggcc gaagggaatc ggaatgtcac ggccgcgccg cgcgagaatc
      121 atcagcacga tgccgaccat cgtgcctacc accgacgaca gcaggacgat cggcagcagc
      181 atcttccagc cgaaccaggc gccaagcgcg gcgagaagct tgaagtcgcc gtgacccatg
      241 ccctccttgc cggtgaggag cttgaacagc cagtagatcg accacaagat caggtatccg
      301 gccaccgcgc cgatcacggc atcgcgcagc ggcgcaaagg tgtcgatcgt attggccagc
      361 aggccgagcc agagcagcgg cagcgtcaca ctgtccggca gcagcgtcgt gtccgcatcg
      421 atgaaggtga gcgcgatcaa cgcccacacg aagaaaagcg caagcaaacc ctggctgccg
      481 acgccgaagt gccaggcggt ccacgcagac agcgccgcgg tcagtccctc gacgatcgga
      541 tagcgcggcg agataggggc gccgcacgcc gcgcatctac cgcgcaggtg caggtaggaa
      601 atcagcggca cgttgtcgcg gatgcgcagc ggggtcttgc aggccggaca gtgtgaggct
      661 ggaacgaaca ggttgtaggg accgctttca gccggcgcct tgccgtcgag ttcggcgcat
      721 tgcgcgcgcc agtccgcttc catcatgcgc ggaacgcgat ggatcacgac gttcaggaaa
      781 gagccgacga ccagtcctac gaggatcgcg gcgagcacga agaactgatc gggcgacacg
      841 gcgggcgccg gagacgcagc gcgcctccgg cccgaaatcg gacggtcaga tcacctggcc
      901 cagcttgaag atcggcaggt agatcgcgat cacgatgccg ccaaccagca cgccgagaac
      961 gaccatgatt gccggttcga tcaggctcga gatcgcgtcg accatctcgt cgacctcgcg
     1021 ctcgtagtag tccgctacct ttgaaagcat cgagtcgagc gagccggatt cctcgccgat
     1081 cgatgtcatc tgcaccgcca tgctggggaa cacggccgag ttctgcattg cctgggcgag
     1141 gctggtgccg atgttgacct cggtttgaat ctgcttggtg gcttcacggt aaaccgcgtt
     1201 gcccgaggcg ggaccgaccg agtcgagcgc ctctaccaat ggcaccccgg cggcgaacgt
     1261 cgtcgacagc gtgcggcacc agcgcgcgac cgaagccttt tgcagcagtt cgccgaccac
     1321 tggcggtttc agcatcaatc tgtcgaccag gatctggaag ttcggcgagc gcttccacgc
     1381 ctggaacacg aagtacggca gaccgatcag gaaggcgagc aacacgtacc agtagtcgac
     1441 cacaaagtcc gagatggcga tcaccgccag agtcggtgcc ggcaagtccg caccgaagct
     1501 tctgaagacc tgcttgaagc tcggaatgac gaaatacatg atgatcgcgg tgatcagaat
     1561 ggccaccagg atgaccatga ttggatagaa catcgccttc ttgatcttcc ccttcagcgc
     1621 gagcgtcttc tccttgtacg tcgccagccg gtcgagcagc gtctcgagaa tgcccgcctg
     1681 ttcgcctgca gccacgaggt tgcagaacag tggatcgaag tacagcggat acttgcggaa
     1741 cgcctgattc aggctggttc cggtttcgac gtcggcgcgg atgtcattga gcagccgcgc
     1801 catcgacgga ttgccgtggc cgcgtgcgac gatgtcgaag cactgcatca gcggcacgcc
     1861 agcgcgcagc atggtcgcca gttgtcgcgt gaagatcgcc agatccttgt cgctgatccg
     1921 cctgccgcgc gaaaaggtct gcttcttgat cttcgtcgcc agcacgccgc gccggcgcag
     1981 cgaagcggcg acgaccgact cgccgccggc acgcatctcg ccgcgcacga ccttgccgtt
     2041 cttgtcgcgg ccttcccagg tgaagagcgt ctcgcgcgcg ggcgctcggg gtcggatcag
     2101 ggcgccagtt gccatgtgct cgtttgctcc gctttcgatc agtcgttggt gcagcccagt
     2161 acttcgtcaa ccgaggtgat gccctgcttg accttcagca agcccgactg cctcagattc
     2221 ttcacgcctt cgcgcttggc ctgcgcggca atctcgaccg agcttgaatt cttcaggatc
     2281 aggcgctgga t
//
LOCUS       DBNHBY010000791         2493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_62166, whole genome shotgun sequence.
ACCESSION   DBNHBY010000791 DBNHBY010000000
VERSION     DBNHBY010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_62166"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..454
                     /gene="rplK"
                     /locus_tag="AC16HB_18545"
     CDS             23..454
                     /gene="rplK"
                     /locus_tag="AC16HB_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR5608846.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPIPVVITAFADKSFTFVMKTPPATVLIKKAAKIEKGSPKPHTDKVGKIT
                     RAQAEEIAKTKMPDLTAANMDSAVRTIAGSARSMGITVEGL"
     gene            455..1150
                     /gene="rplA"
                     /locus_tag="AC16HB_18550"
     CDS             455..1150
                     /gene="rplA"
                     /locus_tag="AC16HB_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5608847.1"
                     /translation="MAHLSKRTKTMRGKVEVNRSYALAEALKLVKETATAKFDESVDV
                     AVVLGIDAKKSDQSVRGAVVLPGGTGKTVRVAVFTQGAKAEEAKTAGADIVGFDDLAA
                     KVKDGFMDFDVVIASPDAMRVVGALGQILGPRGLMPNPKVGTVTPDVAGAVKNAKAGQ
                     VQFRANKEGVVHCSIGRASFEIDRLQSNMTALMDALNKAKPASVKGIFLKKLSVSSTM
                     GIGVRVDPASLSG"
     gene            1477..2007
                     /gene="rplJ"
                     /locus_tag="AC16HB_18555"
     CDS             1477..2007
                     /gene="rplJ"
                     /locus_tag="AC16HB_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5608848.1"
                     /translation="MGLNREEKAAMVDEVSALVAKAQSIVVAEYRGLTVESVTKLRKE
                     ARAQGVQLRVLKNTLARRAVGGTPFAGLADKLVGPLVYGFSSDPVAAAKVIANFAKGN
                     DKLVVKAGAMPNYVMDVKGVTALATMPSREELLAKLMATMQAPIGQFVRTLNEVPSRF
                     VRTLAAVRDAKEKAAA"
     gene            2056..2436
                     /gene="rplL"
                     /locus_tag="AC16HB_18560"
     CDS             2056..2436
                     /gene="rplL"
                     /locus_tag="AC16HB_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5608849.1"
                     /translation="MALSNQEILDAIAAKSVLEISELIKMMEDKFGVSAAAAAVAVAA
                     PAGGAAAPAAEEKTEFTVVLAETGANKVNVIKAVRELTGLGLKEAKDLVDGAPKPVKE
                     GVNKADAEAAKKKLEEAGAKVEVK"
BASE COUNT          518 a          717 c          852 g          406 t
ORIGIN      
        1 ttgaactcag gaaagagctg acatggcaaa gaagatcgtc ggctttatca agctgcaggt
       61 gccagccggt aaagccaatc cgtcgccgcc gatcgggccc gcgctcggtc agcgcggcct
      121 gaacatcatg gaattctgca aggcgttcaa cgcccagacc cagggcgtcg agcccgggct
      181 gccgattccg gtggtcatca cggcattcgc ggacaagagc ttcacgttcg tgatgaagac
      241 gccgccggcc acagtgctga tcaagaaggc ggcgaagatc gagaagggct cgccaaaacc
      301 gcataccgac aaggtcggca aaatcacgcg cgcgcaagcc gaggagatcg cgaagaccaa
      361 gatgccggac ctcaccgccg ccaacatgga ttcggcggtg cgcacgatcg ccggcagcgc
      421 gcgctcgatg ggcatcacgg tggagggact gtaaatggca catctctcca aacgaaccaa
      481 gacgatgcgc ggcaaagtcg aagtgaacag gagctacgcg ctcgcggagg cgctgaagct
      541 cgtgaaggaa accgcgaccg cgaagttcga cgagtcggta gacgtcgcgg tcgtactggg
      601 catcgacgcg aagaaatcgg accagtccgt gcgcggcgcc gtcgtgctgc ccggcggcac
      661 cggcaagacc gtgcgcgtgg ccgtgttcac gcagggcgcg aaggccgaag aggccaagac
      721 cgcgggcgca gacatcgtcg gattcgacga cctcgccgcg aaggtcaagg acggtttcat
      781 ggacttcgat gtcgtgatcg cgtcgcccga tgcgatgcgc gtggtgggcg cgttgggcca
      841 gattctcgga ccgcgcgggc tgatgccgaa cccgaaggtc ggtacggtga ctccggatgt
      901 ggccggcgcg gtgaagaacg ccaaggctgg ccaggtccag ttccgtgcca acaaggaagg
      961 cgtggttcac tgttcgatcg gccgcgcgtc tttcgagatc gatcggctgc agtcgaacat
     1021 gactgcactg atggacgcgc tgaacaaggc gaagccggca tcggtcaagg gcatttttct
     1081 gaagaaactt tcagtgtcgt cgacgatggg aattggcgtg cgcgtcgatc ctgcgtcgct
     1141 gagcggatag gaagccatgc ccggctgcga ccgggcagga atccgatgag tccggttggc
     1201 ggactcatcc aagggctttg ggctgcgtgt ctcgtggaga tgtcttcgat acgggcgcgc
     1261 cgctgtcaaa gaccgctggg gtctctcggt ggtgctttag ccagggactt aagcaagccg
     1321 gcgcgagccg gcgaacccag cgcagacggt gtcccgaaga gagcaatttg cgttgtgaag
     1381 cgttcgcgcc tgcaactcga aggactctcg cagtggtcgc cggtttagca accggtgcaa
     1441 ggcgtgagct gagcaccttt gagtggagcc agtaccgtgg gtctcaatcg agaagagaaa
     1501 gcggccatgg tcgatgaggt ctccgccctc gtggcgaagg cccagtcgat cgtcgtcgcc
     1561 gagtaccgtg gactgactgt ggaatcggtc accaagctgc gcaaggaggc gcgcgcgcaa
     1621 ggtgtccagt tgcgtgtgct gaagaacacg ttggcgcgcc gcgccgtcgg gggcacgccg
     1681 ttcgccggat tggccgacaa gctcgttggt ccgctggtgt acggcttcag cagtgacccc
     1741 gtggcggccg ccaaggtgat cgcgaacttc gccaaaggca acgacaagct cgtcgtcaag
     1801 gccggcgcca tgcccaacta cgtaatggac gtgaagggcg tgaccgcact tgccacgatg
     1861 ccgagccgcg aggaattgct ggcgaagctg atggcgacca tgcaggcgcc gatcggccag
     1921 ttcgtgcgca cattgaacga agtgccgtcc cgcttcgttc gcactctcgc cgccgtgcga
     1981 gatgcgaagg aaaaggcggc ggcctgatcc gcattcagtc ccgaatccgc attcacaacg
     2041 cattaggagt agcaaatggc actttcgaat caggaaatcc tcgacgcgat cgccgcgaaa
     2101 tccgtcctcg aaatcagcga gctgatcaag atgatggaag acaagttcgg cgtttcggct
     2161 gccgcggccg cggtggccgt tgccgccccc gccggcggtg cggctgcgcc ggcagcggaa
     2221 gagaagaccg agttcacggt cgttctggcg gagaccggcg ccaacaaggt caacgttatc
     2281 aaggccgtgc gtgaactcac gggtctgggc ctgaaagagg ccaaggatct ggtcgacggc
     2341 gcgcctaagc ccgtgaagga aggcgtgaac aaggccgacg ccgaggctgc gaagaaaaag
     2401 ctggaagaag ccggcgccaa ggtcgaggtg aagtaatgca gcaatgccgg tgcgccttcg
     2461 gcgcaccggc gccgaaggct tttgttttgg ggc
//
LOCUS       DBNHBY010000792         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1416132, whole genome shotgun sequence.
ACCESSION   DBNHBY010000792 DBNHBY010000000
VERSION     DBNHBY010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1416132"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..827)
                     /locus_tag="AC16HB_18565"
     CDS             complement(162..827)
                     /locus_tag="AC16HB_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5608850.1"
                     /translation="MNNLLRRAACCAARLVATPLFAAPAADPAFDNELLALQQAWART
                     NYETPAGDARVQAFDALEKRAEAFTRSHPNRAEAYIWEGIIESSYAGAKGGLGALSLC
                     KEAKGNLEAALKIDPKALDGSAYTSLGTLYDQVPGFPIGFGDHDKAEKMLKKALELNP
                     NGIDPNYFYGQYLYGRERYQEALQYLDRAAKAPPRPGRESADKGRHAEIAALRTKVQA
                     KLS"
     gene            complement(848..1663)
                     /locus_tag="AC16HB_18570"
     CDS             complement(848..1663)
                     /locus_tag="AC16HB_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5608851.1"
                     /translation="MKSGDMRAVVTGASGGIGGAIATELARRGAAVLLVGRDLASLER
                     CAASAPGADAGRVTVCVADLATVAGRERVAEAARAFGANVLVNNAGRGEFALVEAQTD
                     AALEQLFAINALAPIQLTRALLPHLRAQPAAAVLNVGSVFGSLGYPGFAAYSATKFAL
                     RGYSEALRRELADSNVTVHYLAPRATRTGFNASAVERMNAELGVAMDPPGTVAAAACA
                     MLERGGLDAVIGWPEKLFVRINSLLPRVVDGSLRKQLPVIRRYATEAAAGEPR"
     gene            complement(1660..>1965)
                     /locus_tag="AC16HB_18575"
     CDS             complement(1660..>1965)
                     /locus_tag="AC16HB_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR5608852.1"
                     /translation="GMVYVLEGTSVALALHAADRIQARLQLPDTAFSYLRSHGQLDQE
                     HVGDLAAILERFTDPADCEAVLRCARGIYWLYGNVFRGLDDASPVMAPVAAPRKVSA"
BASE COUNT          281 a          693 c          679 g          312 t
ORIGIN      
        1 gagcgcttcg gcgtccggcg cctgctgctg gcatgcacgc gcctggagtt cgcgaccccg
       61 ctcaacccga cgccgctgcg ggtggaggct cccctcgccc gggagtttgc cgcgctcgtc
      121 gatgcactcg gcaacgacta cgcggcaacc ccggcgctcg cttagctgag cttggcctgc
      181 accttggtgc gcagcgccgc gatctcggca tggcgtccct tgtcggcaga ctcgcgaccg
      241 gggcgcggcg gcgccttcgc ggcgcggtcg aggtactgca gggcctcctg gtagcgctcc
      301 cgaccgtaca ggtattggcc atagaagtaa ttcgggtcga tcccgttcgg gttcagctcg
      361 agcgctttct tcagcatctt ctcggccttg tcgtggtcgc cgaagccgat cgggaagccg
      421 gggacctggt catacagcgt gccgaggctg gtatacgccg atccgtcgag cgccttcgga
      481 tcgatcttca gcgccgcctc gagattgccc ttcgcttcct tgcacagcga cagcgctccg
      541 aggccgccct tcgcgccggc gtaactgctc tcgatgatgc cttcccagat gtaggcctcg
      601 gcacgattgg ggtgcgacct ggtgaaggct tcggcacgct tctccagcgc gtcgaaggcc
      661 tggacacgcg cgtcgcccgc gggcgtctcg tagttcgtcc gggcccacgc ctgctgcaag
      721 gcgagcagtt cgttgtcgaa ggccggatcg gcggccggcg ccgcgaacag cggcgttgcg
      781 accagccggg cagcgcagca tgcggcgcgg cgcaacaggt tgttcatgtc aggttcctcc
      841 ttggggatca gcggggttcg ccggccgcgg cttcggtcgc gtaccggcga atgacgggga
      901 gttgcttgcg cagcgagccg tcgacgaccc gcggcagaag tgaattgatg cgtacgaaca
      961 gcttctcggg ccagccgatc acggcgtcca gtccgccgcg ttccagcatg gcgcacgcgg
     1021 cggctgccac cgtcccgggc gggtccatgg cgacgcccag ctcggcattc atgcgttcga
     1081 cggccgatgc gttgaaaccc gtccgcgttg cacgcggcgc gaggtaatgc acggtgacgt
     1141 tcgagtcggc gagttcacgg cgcagcgcct cgctgtagcc gcgcagcgcg aacttggtgg
     1201 ctgaataagc cgcgaatccc gggtagccca ggctgccgaa taccgagccg acgttgagca
     1261 cggcagccgc cggctgcgcg cgcaggtgcg gcagcagcgc gcgcgtcagc tggatcggcg
     1321 cgagcgcgtt gatggcgaac agctgctcga gcgccgcgtc ggtctgcgcc tccactagcg
     1381 cgaattcacc gcggccggcg ttgttgacca gcacgttggc gccgaaggca cgcgccgcct
     1441 ccgccacgcg ctcgcgcccg gcgaccgtcg cgagatctgc aacgcacact gtgacccgac
     1501 ctgcatcggc gcccggcgcg gaggcggcgc accgctccag gctggcgagg tcgcggccga
     1561 ccagcagcac cgctgcacca cggcgcgcca gctcggtcgc gatcgcaccg ccgatcccac
     1621 cgctggcgcc ggtgaccacc gcacgcatgt caccggactt catgccgaca ccttccgcgg
     1681 tgccgcgacg ggcgccatca cgggcgaggc gtcgtcgagg ccgcgaaaca cgttgccata
     1741 cagccagtag atgccgcgcg cgcagcgcag cacggcctcg cagtccgccg ggtccgtgaa
     1801 gcgctcgagg atcgcggcca ggtccccgac gtgctcctgg tcgagctgcc cgtggctgcg
     1861 caggtaactg aacgcagtgt cgggcaactg cagcctggcc tggatgcggt ccgcggcgtg
     1921 cagggcgagc gccacgctgg tgccttccaa cacgtagacc atgcc
//
LOCUS       DBNHBY010000793         5594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1821461, whole genome shotgun sequence.
ACCESSION   DBNHBY010000793 DBNHBY010000000
VERSION     DBNHBY010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1821461"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16HB_18580"
     CDS             complement(<1..638)
                     /locus_tag="AC16HB_18580"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HIR5608853.1"
                     /translation="MHDAPLHARIGRTWPGPMFGTADRSRRPRAALRTAWLLLAVLLL
                     ALWPHWVWLARRLTDGSDEPWGIVSIVAVLVLVARDRHALSMPARHALAASGALAVTA
                     AVLQLFVPPIFAAGIAMLSLALFLASTLPQRPAAPLATLLLLALPVIASLQFYFGFPL
                     RVLTAHAAAPLLWAVRIDAHAAGASLQWNGATVLVDPPCAGIAMLWVGSFVAA"
     gene            complement(631..2637)
                     /locus_tag="AC16HB_18585"
     CDS             complement(631..2637)
                     /locus_tag="AC16HB_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and vWA domain-containing protein"
                     /protein_id="HIR5608854.1"
                     /translation="MFNFLRLLLLLSIAGAAYAQTPGDAARPHSPYFVVAGSQPGVDA
                     LPLKSTRADVTISGVIADVRVTQRYRNEGATPIEARYVFPASTRAAVYAMRLRVGDRV
                     VDAEVREKQQARRDYDKAKQEGRSAALLEQQRPNVFSMAIANVMPGDEIAVDLRYTET
                     IVPDEGIYRFVFPTVVGPRYNGGTEPDGTPGESRKNEPWIAQPTLRAGQPAPHTFELN
                     ATVLAPMPVKMLASPSHALDIDGVGEKKATARLAPDRAHATRDVVLEYRLAGAAIESG
                     VLLLQDQDENFFLLLAEPPARVAESSLPPREYVFILDISGSMHGFPLATAKSLLRQLV
                     QRLRPGDTFNVIPFAGGSSLLAPQSLPASADNIAAAIRFIDGQTGGGGTELLPALRRA
                     LALPADPGRARTFAVITDGYVSIEKEAFALVRANLGNANVFAFGIGSSVNRFLIEGLA
                     RAGKGEPFFVLDAAQAEREAMRFRQMIEQPVLTRIRVKFPAGFDAYDLDVPQAPDLFA
                     QRPVVLMGNYRGTPTGTIEVEGISAGGAVRLPVDLERGAPSNDTQALKYLWARSRIAA
                     LGDDLKLRADDDTRQAITALGLKYNLLTDFTSFIAIDKVVRNVGGGSTSVDHPQPLPE
                     GVSELALAAAPATPEPEFALLAALAGGLTWWLRRRRLTVEIRHA"
     gene            complement(2656..3420)
                     /locus_tag="AC16HB_18590"
     CDS             complement(2656..3420)
                     /locus_tag="AC16HB_18590"
                     /inference="COORDINATES: protein motif:HMM:NF025132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608855.1"
                     /translation="MLGQTIAEGALRAAQLVPLLFALNTPAPAEAQLPRPAPAEPPFA
                     VEAEQSPALTLTEHHVDAHVRGGQAQVRSLLTYRNERAEPVSASFAFPFPALLQQGDM
                     WRTLGTESVEAADGGDESPEDAEFREAGEPAPSRIDVGYVTVAPGEEIRIETQRAIDL
                     APRARGWRLALPLPVERSAPYAPQFSADVSVEESPAVARLASTTHGGTATGIGSPRAQ
                     LEVPAGRVYLGAQFVVDIDVGAAGDIALWGGEAHTR"
     gene            complement(3538..4989)
                     /gene="creC"
                     /locus_tag="AC16HB_18595"
     CDS             complement(3538..4989)
                     /gene="creC"
                     /locus_tag="AC16HB_18595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HIR5608856.1"
                     /translation="MSTRSRIFIGIFVVFLAAIGVLLYRVAADFDPRYRESAEESLVD
                     TANLLATLLERRTYDGVIPTDELERTLAELTRRPVYARIFDVEKTRIDLHVYVTDRDG
                     TVLFDSKGKDVGANYLDWRDVSRTLEGQYGARTTVVVPGDPDSAVMYVGAPIRERTAA
                     SPAGDIVGMVSVGKPIASIRPFIVNAREKLALVGVISTAGFALLMVFATVWLVRPFGF
                     VRDVWRTLARDPGSIGSFGRRFVHALRTAFADMRDALAGRSYVDQYVQTLTHELKSPL
                     AAIRGAAELLREPMPENARARFAGNIEEQVQRAQMLIDRLLELSGLERRAALDRSEPV
                     SLVQAAYAARDELATLAAQKEIEIAIAADASATVRGDAFLLQRAAANLLANAIDFAPR
                     FSKIEIAVRALRKHVELTVRDRGPGLPEYARDRVFEKFFSLARPDSGKKSTGLGLALV
                     REVAALHGGSARLANHPDGGAIATLTLPRPKSP"
     gene            complement(4993..>5594)
                     /gene="creB"
                     /locus_tag="AC16HB_18600"
     CDS             complement(4993..>5594)
                     /gene="creB"
                     /locus_tag="AC16HB_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HIR5608857.1"
                     /translation="QWVATGAEALAALDKQPCVLAILDVGLPDINGFNLFKRVSARHP
                     GLPAVFLTARTAEVDRVVGLELGADDYVAKPFSPRELVARVRAVLRRSNRPSLPADSS
                     PATPFLVDDERKVIRYRGAVLELPRIEYRLLKVLIERPGRVYSRDELMSRAWDDPGSS
                     FDRTVDTHIKSLRAKLRAIAPDDDPIQTHRGLGYSLREQ"
BASE COUNT          835 a         1944 c         2014 g          801 t
ORIGIN      
        1 gcggcgacga agctgccgac ccacagcatg gcgatgccgg cgcacggcgg gtcgaccagc
       61 accgtggcgc cgttccactg cagcgacgcg ccggccgcgt gcgcgtcgat gcggacggcc
      121 cacagcagcg gcgcggccgc atgcgcggtc agtacgcgca gcggaaagcc gaaatagaac
      181 tgcagcgaag cgatcaccgg caatgcgagc agcagcaacg tggcgagcgg cgcggccgga
      241 cgctgcggca acgtcgacgc gaggaagagc gccagcgaaa gcatggcgat gcccgcggcg
      301 aagatcggcg gcacgaaaag ctgcaggacc gccgccgtga ccgcaagcgc accgctggcc
      361 gcgagcgcat ggcgtgccgg catcgacaga gcgtgccggt cacgcgccac tagtaccagc
      421 acggcgacaa tcgaaacgat gccccacggc tcatcggagc cgtcggtaag gcggcgcgcc
      481 agccacaccc agtgcggcca caacgccagc agcagcacgg cgagcaggag ccacgcagtc
      541 cggagcgctg cccgtggccg acgcgacctg tccgccgtgc cgaacatcgg cccgggccat
      601 gtgcggccga tgcgcgcatg caacggcgcg tcatgcatga cggatctcca ccgtcaaacg
      661 gcgccggcgc agccaccacg tcagcccgcc tgcgagtgcc gcgagcagcg cgaactccgg
      721 ctccggggtg gcgggtgcgg cggcgagcgc gagttcgctg acgccctcgg gcagcggttg
      781 cgggtggtcg acgctcgtcg acccgccgcc cacattgcgc acgaccttgt cgatcgcgat
      841 gaacgatgtg aagtcggtca gcaggttgta cttcagcccg agcgcggtga tcgcctgtcg
      901 ggtgtcgtca tcggcgcgca gtttcaggtc gtcgcccagc gctgcgatgc gactgcgcgc
      961 ccatagatac ttcaacgcct gcgtgtcgtt cgacggcgcg ccgcgctcca gatcgaccgg
     1021 cagccgcacc gcgccgccgg cggagatgcc ttcgacctcg atcgtgccgg tcggtgtgcc
     1081 gcggtaattt cccatcagca cgaccggccg ctgcgcgaac agatccggcg cctgcggcac
     1141 gtcgaggtcg taggcgtcga acccggcggg gaacttcacg cggatccgcg tcagcacggg
     1201 ttgctcgatc atctggcgaa agcgcatcgc ctcgcgctcg gcctgcgcgg catcgagcac
     1261 gaagaacggc tcgcccttgc ccgcgcgcgc cagcccttcg atcaggaagc ggttgaccga
     1321 ggagccgatg ccgaacgcga acacgttggc gttgccgagg ttcgcgcgca ccagcgcgaa
     1381 tgcctccttt tcgatcgaca cgtagccgtc ggtgatcacc gcgaatgtgc gcgcacggcc
     1441 gggatcggcc ggcagggcga gcgcgcgccg cagcgccggc agcagttccg tgccgccgcc
     1501 accggtctgc ccgtcgatga agcggatcgc ggccgcgatg ttgtccgcgc tcgccggcag
     1561 agattgcggc gccagcagcg acgagcctcc ggcgaaggga atcacgttga acgtgtcgcc
     1621 cgggcgcagc cgctggacga gctggcgcag cagcgacttt gcggtcgcga gcggaaagcc
     1681 gtgcatcgag ccggagatgt cgaggatgaa cacgtactcg cgcggcggca ggctgctctc
     1741 cgccacgcgg gccggcggct cggcgagcag caggaagaag ttctcgtcct ggtcctggag
     1801 cagcagcacg ccgctttcga tcgcggcccc cgcaagccgg tactcgagca ccacgtcgcg
     1861 ggtcgcatgc gcgcggtcgg gggccagccg cgcggtcgcc ttcttctcgc cgacgccgtc
     1921 gatgtcgagc gcgtgcgacg gtgacgccag catcttcacg ggcatcggcg ccagcacggt
     1981 cgcgttcaat tcgaacgtgt gcggtgccgg ctgcccggcg cgcagcgtcg gctgcgcgat
     2041 ccatggctca ttcttccggc tttcgcctgg cgtgccgtcc ggctccgtgc cgccgttgta
     2101 gcgcggcccg acgacggtcg ggaacacgaa gcgatagatt ccctcgtccg ggacgatcgt
     2161 ctccgtgtac cgcaggtcga ccgcgatctc gtcgccgggc atcacgttcg cgatcgccat
     2221 gctgaagacg ttcggccgct gttgctcgag cagcgcagcg ctcctgcctt cctgtttcgc
     2281 cttgtcgtag tcgcgccgtg cctgctgctt ctcgcgcacc tcggcgtcga ccacgcggtc
     2341 gcccacgcgc agccgcatcg cgtacaccgc ggcgcgcgtc gacgcgggga atacgtagcg
     2401 cgcctcgatc ggcgtggcgc cttcattgcg gtaccgctgc gtgacccgca cgtcggcgat
     2461 cacgccgctg atcgtcacgt cggcgcgggt ggacttcagc ggcagcgcgt cgaccccggg
     2521 ctggctgcct gcgacgacga agtagggcga atgcggccgc gcggcgtcgc cgggggtctg
     2581 cgcgtacgca gcgccggcga tcgacagcag caacagcagg cgaaggaagt tgaacatggc
     2641 ggtctcgtcc gaggctcagc gcgtgtgcgc ctcgccgccc cacagcgcga tgtcgcctgc
     2701 cgcgccgacg tcgatgtcga ccacgaactg cgcgccgagg tacacgcggc cggcggggac
     2761 ctcgagctgc gcgcgcgggc tgccgatgcc ggtggcggtg ccgccatgtg tcgtcgacgc
     2821 cagtcgcgcc acggccgggc tttcctccac cgagacgtcg gcggagaact gcggcgcata
     2881 gggcgcgctg cgctcgaccg gcagcggcag ggcgaggcgc cagccgcggg cgcgcggcgc
     2941 gaggtcgatc gcgcgctgcg tctcgatccg gatctcctcg ccgggtgcga ccgtcacgta
     3001 acccacatcg atgcgcgacg gtgcgggctc gccggcctcg cggaattcgg cgtcctccgg
     3061 cgattcgtcc ccgccgtcgg ccgcctcgac cgactccgtg ccgagcgtgc gccacatgtc
     3121 gccctgctgc agcagcgccg ggaacgggaa cgcgaagctg gcggacaccg gctccgcgcg
     3181 ctcgttgcgg taggtgagca gcgagcggac ctgcgcttgt ccgccgcgga cgtgcgcgtc
     3241 gacgtggtgt tcggtcagcg tcagtgcggg ggactgctcg gcctcgaccg cgaatggcgg
     3301 ctcggccggc gccgggcggg gcagctgtgc ctccgcaggc gcaggagtgt tcaaggcaaa
     3361 cagcagcgga accagttgcg cggcgcgcag cgcgccttcg gcgatcgtct gacccagcat
     3421 cgtgttctcc tcgtcgtgac ccgcggatcg gcgggcctgc ggggagaact gtcggcgcgg
     3481 cgcgtgaggc gggttcgaac cgcacgtgcg ccgcgcgtga agcctgtgtg aagtccgtta
     3541 cggcgacttc ggccgcggca gcgtcagcgt cgcgatcgcg ccgccgtcgg gatgattggc
     3601 gagccgcgcg gatccgccgt gcagcgcggc aacctccctg accagcgcca ggccgaggcc
     3661 ggtgctcttc ttgccgctgt cggggcgcgc gagcgagaag aacttctcga acacgcggtc
     3721 gcgcgcgtac tcgggcagcc ccgggccgcg gtcccgcacg gtgagttcga cgtgcttcct
     3781 cagcgcgcgc acggcgatct cgatcttcga gaaccgcggc gcgaagtcga tcgcattggc
     3841 gagcaggttc gcggccgcgc gctgcagcag gaacgcgtcg ccgcggacgg tcgcgctggc
     3901 gtcggcggcg atcgcgatct cgatttcctt ctgcgcggcg agcgtggcga gttcatcgcg
     3961 cgccgcatag gcggcctgca ccagcgacac cggttcgctc cggtcgagcg cggcgcggcg
     4021 ctccagaccc gacaactcca gcaggcggtc gatcagcatc tgcgcgcgct gcacctgctc
     4081 ctcgatgttg ccggcgaagc gcgcgcgcgc gttctccggc atcggctcgc gcagcagttc
     4141 ggcggcgccg cggatcgcgg cgagcgggct cttcagctcg tgggtcagcg tctgcacgta
     4201 ctggtcgacg tagctgcgtc cggcgagcgc gtcacgcatg tcggcgaacg cggtgcgcag
     4261 cgcgtgcacg aagcggcggc cgaagctgcc gatcgagccg ggatcgcgcg ccagcgtgcg
     4321 ccacacgtcg cgcacgaaac cgaacggccg caccagccac acggtggcga acaccatcag
     4381 cagcgcgaag ccggcggtcg agatcacgcc gacgagcgcg agcttctcgc gcgcattgac
     4441 gatgaagggg cggatcgaag cgatcggctt gccgacgctg accatgccga cgatgtcccc
     4501 ggccgggctg gccgcggtcc gctcgcggat cggcgcgccg acatacatca ccgccgagtc
     4561 cgggtcgccc ggcacgacga ccgtcgtgcg ggcgccgtac tgtccctcga gcgtgcggct
     4621 gacgtcgcgc cagtcgaggt agttggcgcc gacgtccttg cccttggagt cgaacagcac
     4681 ggtgccgtcg cggtcggtca cgtagacgtg cagatcgatc cgcgtcttct cgacgtcgaa
     4741 gatgcgcgcg tacaccggcc ggcgcgtcag ctccgccagc gtgcgttcga gctcgtcggt
     4801 cgggatcacg ccgtcgtagg tgcggcgctc cagcagcgtg gcgagcaggt tcgcggtgtc
     4861 gaccagcgac tcctcggcgc tttcgcggta gcgcggatcg aagtcggccg ccacgcgata
     4921 cagcagcacg ccgatcgcgg ccagaaagac gacgaagatg ccgatgaaga tgcgcgagcg
     4981 cgtcgacatc ggctactgct cgcgcagcga atagccgagc ccgcgatggg tctggatcgg
     5041 atcgtcgtcg ggggcgatcg cgcgcagctt ggcgcgcagt gacttgatgt gcgtgtcgac
     5101 ggtgcggtcg aagctcgatc cgggatcgtc ccaggcgcgg ctcatcagct cgtcgcgcga
     5161 gtagacgcgg ccggggcgct cgatcaggac cttcagcagc cgatactcga ttcgcggcag
     5221 ctcgagcacc gcgccgcggt agcggatgac cttgcgttcg tcgtccacaa ggaagggagt
     5281 cgccggcgat gaatccgccg gcaacgacgg cctgttcgat cttcgcagga cggcacggac
     5341 gcgggcgacc agttcgcgcg gcgagaatgg cttggcgaca tagtcgtcgg cgccgagttc
     5401 caggccgacg actcggtcga cctcggcggt gcgcgcggtc aggaataccg caggcaggcc
     5461 cggatgccgg gcactgaccc gcttgaacag gttgaagccg ttgatgtcgg gcaggccgac
     5521 gtcgaggatc gccagcacac acggctgttt gtccagcgcc gccagcgcct cggcgccggt
     5581 ggcgacccat tgca
//
LOCUS       DBNHBY010000794         4087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1416199, whole genome shotgun sequence.
ACCESSION   DBNHBY010000794 DBNHBY010000000
VERSION     DBNHBY010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1416199"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC16HB_18605"
     CDS             <1..547
                     /locus_tag="AC16HB_18605"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5608858.1"
                     /translation="LLISSIECDDPVIFLEPKRLYNGPFDGHHDRPVVPWSKHPRGEV
                     PEDYYTVPLESAAVFRAGQDLTVLTYGTMVWVSEAAANETGVDVEIIDLRSLWPLDLD
                     TIVASVKKTGRCVVVHEATRTSGFGAELAALVQEHCFYHLEAPIERVTGWDTPYPHAQ
                     EWAYFPGPQRVGEAFKRTMEA"
     gene            549..1844
                     /locus_tag="AC16HB_18610"
     CDS             549..1844
                     /locus_tag="AC16HB_18610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5608859.1"
                     /translation="MGIHVIKMPDIGEGIAEVELVEWHVQPGDAVVEDQVLADVMTDK
                     ATVEIPSPVRGKVVSLGGQVGEVMAVGTELLRLEVEGAGNVAEGAPPIAARAPAPAAA
                     PKPAPAPAAAPKQAPAAAPKPAPPPAPSAPAAPPVRRAPGERPIASPAVRRRAWDLGV
                     ELQFVQGSGAAGRIMHADLDAYLARGDAVVTTGARYVERNDEQPIQVVGLRRKIAEKM
                     QEAKRRIPHFTYVEEIDVTELEALRARLNETYAGERGKLTLLPFLMRAMVLAVRAYPM
                     VNARFDDDNGVVTRYGAVHLGIATQTDAGLMVPVVRHAEARDLWSSAAEVARLAEAAR
                     TAKATRDELTGSTLTITSLGALGGIVTTPVINYPEVAIVGVGRIIERPVIRNGAIVAR
                     KLMNLSSSFDHRVVDGAHGAEFVQAVRGYLETPAAMFVE"
     gene            1949..3331
                     /gene="lpdA"
                     /locus_tag="AC16HB_18615"
     CDS             1949..3331
                     /gene="lpdA"
                     /locus_tag="AC16HB_18615"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5608860.1"
                     /translation="MQTQSTQLLVIGGGTGGYVAAIRAAQLGIKTVLVEGDRIGGTCL
                     NIGCIPSKALIHVAEEFEKANHYAAGSPLGIRVQKPALDVAQAVQWKDGVVQRLTHGV
                     AGLLKKYGAQVVNGRATIVDGKTVDVADLRIGCEHLVLASGSEPTPLPSLPFGGRVIS
                     STEALSPTGIPKRLVVVGAGYIGLELGIAYRKLGADVTVIEALARVLPQYDAELTKPV
                     EASLRKLGIALHLNSKVLGLNAAGDAVRMQDAQGATSEIGADRVLVAVGRRPRTQGFG
                     LETLALDMNGAFVKVDDQCRTSMRNVWAVGDLTGEPMLAHRAIAQGETVAEIIAGRKR
                     RFAPASIPAICFTDPEIVVSGLSPDEATERGFDHIHAQFPFAGNGRALTLESREGFVR
                     VVARRDNHLILGWQAVGKGVSELAGEFSQSLEMGARLEDVGGTIHAHPTLGEAVQEAA
                     LRALGHAIHV"
     gene            3335..3754
                     /locus_tag="AC16HB_18620"
     CDS             3335..3754
                     /locus_tag="AC16HB_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR5608861.1"
                     /translation="MRQAHTLLRIETHGRWLIDITRRTTNWVESSGMRNGLLTVFIRH
                     TSASLLVQENADPQVQRDLERFLARLVRDGDPLFEHRTEGADDMPAHVRSALTQTQLS
                     IPLADGALALGTWQGIYVYEHRLRDHPREVALHLIGE"
     gene            complement(3797..>4087)
                     /locus_tag="AC16HB_18625"
     CDS             complement(3797..>4087)
                     /locus_tag="AC16HB_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608862.1"
                     /translation="PDFALVYLRNDENDIEIELTLNKGRTEPYTHGTGYGHVAVVVGD
                     LTSTHRELSAAGYEPAPIKEFKRGDELLARFFFIQDPDGYKIEVLERHGHYR"
BASE COUNT          665 a         1342 c         1467 g          613 t
ORIGIN      
        1 actgctgatc tcgtcgatcg aatgcgacga cccggtgatc tttctcgagc cgaagcggct
       61 gtacaacggt ccgttcgacg gccatcacga ccggccggtg gttccgtggt cgaaacatcc
      121 gcgcggagag gtgcccgagg actactacac ggtgccgctc gaatcggccg ccgtgttccg
      181 cgcgggccag gacctgaccg tgctcactta cggcacgatg gtgtgggtgt cggaggcggc
      241 agccaacgag accggcgtcg acgtcgagat catcgacctt cgcagcctgt ggccgctcga
      301 tctggacacc atcgtcgcat cggtgaagaa gacgggccgc tgcgtcgtgg tgcacgaagc
      361 cacgcgcacc agcggcttcg gcgccgaact cgcggcgctg gtgcaggagc actgcttcta
      421 tcacctcgaa gcgccgatcg agcgcgtgac cggctgggac acaccgtatc cgcacgcaca
      481 ggaatgggcg tacttccccg ggccgcagcg cgtcggcgag gccttcaaac ggaccatgga
      541 ggcctgacat gggtatccat gtgatcaaga tgccggacat cggcgaaggc attgccgagg
      601 tggagctcgt cgagtggcac gtgcagcccg gcgacgcggt ggtcgaggac caggtgctcg
      661 ccgacgtcat gaccgacaag gccaccgtcg agattccgtc gccggtcagg ggcaaggttg
      721 tatcgctcgg cggccaggtc ggcgaggtga tggcagtcgg aaccgaactg ctccggctcg
      781 aagtcgaagg cgcgggcaac gtggcggagg gtgcgccccc gatcgcggcg cgtgcgcctg
      841 ccccggccgc ggcgcccaag ccggcaccgg ctcccgcggc cgcgccgaag caggcgcccg
      901 cggcggcgcc gaagccggca ccgccgcccg cgccctcggc accggctgca cctccggtgc
      961 ggcgggcgcc gggagaaagg ccgatcgctt cgcccgcagt tcggcgccgc gcatgggacc
     1021 tcggcgtcga actgcagttc gtgcagggga gcggcgcggc gggccggatc atgcacgcgg
     1081 acctcgacgc ctatctggcg cgcggcgacg cggtcgtcac gacgggcgcg cgctatgtcg
     1141 aaaggaacga cgagcagccg atccaggtcg tgggcctgcg gcgcaagatc gcggagaaaa
     1201 tgcaggaggc caagcgccgc atcccgcact tcacctacgt cgaggagatc gacgtcaccg
     1261 aactggaagc gctgcgcgcg aggctcaacg aaacgtacgc cggggagcgc ggcaagttga
     1321 cgctgctgcc cttcctgatg cgcgcgatgg tgctcgcggt gcgcgcctat ccaatggtca
     1381 acgcgcgctt cgacgacgac aacggcgtcg tcacccgcta cggcgcggtg cacctgggca
     1441 tcgcgacgca gacggacgcg ggcctgatgg tgccggtcgt gcggcacgcg gaggcgcgcg
     1501 acctgtggtc ttccgcggcc gaggtcgcgc ggctggccga agcggcgcgc accgcgaaag
     1561 ccacgcgcga cgaactgacc ggctcgacgc tcacgatcac gagcctcggc gcgctcggcg
     1621 gcatcgtcac cacgccggtg atcaactatc cggaggtggc gatcgtcggc gtcggccgca
     1681 tcatcgagcg gccggtgatc cgcaacggcg cgatcgtggc gcgcaagctg atgaacctgt
     1741 cgtcgtcgtt cgatcaccgc gtggtcgatg gcgcgcacgg cgccgagttc gtgcaggcgg
     1801 tgcgcggcta tctcgaaacg ccggcggcga tgttcgtgga ataggcaagg catgtgtcat
     1861 tcccgcgccc gcgggaatcc agtgcctttg ccgacgttga ctggatgcgc gctttcgcgg
     1921 gcatgacaca tccaagcggg gatgatgaat gcaaacgcaa tcgacccaac tgctggtgat
     1981 tggtggcggc accggcggct atgtcgccgc gatccgcgcc gcgcagctcg gcatcaagac
     2041 cgtgctggtc gaaggcgacc gcatcggcgg cacctgtctg aacatcggct gcattccctc
     2101 caaggcgctg atccacgtcg ccgaggaatt cgagaaggcg aaccactacg cggcggggag
     2161 cccgctcggc attcgcgtgc agaagcccgc gctcgacgtg gcgcaggcgg tgcagtggaa
     2221 ggacggcgtc gtgcagcggc tcacgcatgg cgtggccggg ctgctgaaga agtacggcgc
     2281 gcaggtcgtc aacggtcgcg ccacgatcgt cgacggcaag acggtcgacg tggccgatct
     2341 gcgcatcggc tgcgagcacc tcgtgctcgc gtcgggctcc gagccgacgc cgctgccgtc
     2401 gctgccgttt ggcgggcgcg tgatctcgtc gaccgaggcg ctgtcgccga caggcattcc
     2461 gaaacggctg gtcgtcgtcg gtgccggcta catcgggctg gaactcggca tcgcgtatcg
     2521 caagctcggc gccgacgtga cggtgatcga ggcgctcgcg cgcgtgctgc cgcaatatga
     2581 cgcggaactg acgaagccgg tcgaggcgtc gctcaggaag ctgggcatcg cgctgcacct
     2641 gaacagcaag gtgctcggcc tgaacgcggc tggcgacgcg gtgcgcatgc aggacgcgca
     2701 gggcgcgacg agcgagatcg gtgccgaccg ggtgctggtc gccgttggcc gccgcccgcg
     2761 cacgcagggc ttcgggctgg agacgctggc gctcgacatg aacggcgcgt tcgtgaaggt
     2821 cgacgaccag tgccgcacgt cgatgcgcaa cgtgtgggcg gtgggggatc tcaccggcga
     2881 gccgatgctc gcgcaccgcg ccatcgcgca gggcgaaacg gtggccgaga tcatcgccgg
     2941 ccggaaacgc cgtttcgcgc cggcatcgat ccccgcgatc tgcttcaccg accccgagat
     3001 cgtggtgtcg ggcctgtcac ccgacgaggc aacggagcgc ggtttcgacc acatccacgc
     3061 gcagttcccg ttcgccggca atggtcgcgc gctgacgctc gagtcgcgcg agggcttcgt
     3121 gcgcgtggtc gcgcggcgcg acaaccacct gatcctcggc tggcaggcgg tgggcaaggg
     3181 cgtgtccgaa ctcgcgggcg aattctcgca gtcgctggag atgggggcac gactcgaaga
     3241 cgtgggcggc acgatccatg cgcacccgac gctgggcgaa gcggtgcagg aagccgcgct
     3301 gcgcgcgctc ggacacgcga ttcacgtgta gcgaatgcgg caggcccaca cgctgctcag
     3361 gatcgagaca cacggccgct ggctgatcga catcacgcgc cggactacga actgggtcga
     3421 gtcgagcggg atgcgcaacg gcctgctgac cgtgttcatc cgccacacgt cggcgagcct
     3481 cctggtgcag gagaacgccg acccgcaggt gcagcgcgac ctggagcggt tcctcgcgcg
     3541 actggtgcgc gacggcgatc cgctgttcga gcatcggacc gagggtgccg acgacatgcc
     3601 tgcgcatgtg aggagcgcgc tgacgcagac gcagctttcg attccgctcg ctgacggcgc
     3661 gctggcgctc ggcacgtggc agggcatcta cgtgtacgag catcggctgc gcgaccatcc
     3721 acgcgaagtc gcgctgcacc tgatcggcga atagaccgcg cgcgtcgcgg gtcggaggcg
     3781 gatccgggcc gctgcgctaa cggtagtgcc cgtgccgctc gagcacctcg atcttgtagc
     3841 cgtcgggatc ctggatgaag aagaagcgcg cgagcagttc gtcgccgcgc ttgaactcct
     3901 tgatcggtgc cggctcgtag ccggccgcgc tcagttcgcg gtgtgtcgac gtcaggtcgc
     3961 cgaccaccac ggcgacgtga ccgtacccgg tgccatgcgt atacggctcg gtgcgtccct
     4021 tgttcagggt cagttcgatc tcgatgtcgt tttcgtcatt gcgcagatac accagcgcga
     4081 aatccgg
//
LOCUS       DBNHBY010000795         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1282335, whole genome shotgun sequence.
ACCESSION   DBNHBY010000795 DBNHBY010000000
VERSION     DBNHBY010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1282335"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC16HB_18630"
     CDS             complement(<1..502)
                     /locus_tag="AC16HB_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608863.1"
                     /translation="MRPDVGRATAILERAAGEGRADLSATEIASLLAALGIASEVRST
                     AATVELRISLECTREFGLVLSAGAGGLDATLDEQNFRRDRGAVHAAAELTDATDFLRL
                     CRRTIAWQRLVARGQRPDAPLEACVGALLALAKAHPAGAPGARFALRKVVLDFTCAGD
                     TLQVAGA"
     gene            complement(499..1389)
                     /locus_tag="AC16HB_18635"
     CDS             complement(499..1389)
                     /locus_tag="AC16HB_18635"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5608864.1"
                     /translation="MIKLELQDDAMLCSGHAACPGCVEALSVRHILATMGPDTMAVIP
                     PSCMAIIAGPQPYSSLKIPVYQPTLEASAAAASGLRRALDAQGKRDTQVIVLAGDGGT
                     YDIGFQCLSSAAERNEDFIYFCLDNEGYMNTGAQKSSSTPHYASTGSTPAGKTSRKKN
                     LTEIMAAHEVPYVATASVGFLPDLLAKVKKAKSLRGTRIITLLIPCLDGWGVPDDMGL
                     TAARYAVECGAFPLYEVEHGRTWTLNHAQRTRPVGDYLSLQRRYRHLAPAQIEALQDE
                     VDEVWDRLVQKDRASHGAAQ"
BASE COUNT          211 a          536 c          516 g          242 t
ORIGIN      
        1 gcgcaccggc gacctgcagc gtgtccccgg cgcaggtgaa atcgagtacc accttgcgca
       61 gcgcgaacct tgcgcccggc gcccccgccg ggtgtgcttt cgcgagcgcg agcagcgcgc
      121 ccacgcaggc ttcgagcggc gcgtccggac gctgaccacg cgcgacgagc ctctgccagg
      181 cgatcgtgcg tcgacatagc cgcaggaaat ccgtcgcgtc ggtgagttcc gcggcagcgt
      241 gcaccgcgcc gcgatcgcgc ctgaagttct gctcgtcgag cgttgcgtcg agcccgcctg
      301 ccccggcgga cagcacgagg ccgaactcgc gcgtgcattc caggctgatg cgcagttcga
      361 ccgttgccgc agtcgagcgg acctcggacg cgatgccaag cgccgcgagc agcgacgcga
      421 tctcggttgc cgacaggtcc gcgcgcccct cgcccgccgc gcgctcgagg atcgcggtcg
      481 cccgcccgac atcgggtctc actgcgccgc tccgtgcgaa gccctgtcct tctgcaccag
      541 cctgtcccaa acctcgtcga cctcgtcctg cagcgcctcg atctgcgcgg gcgcgaggtg
      601 ccggtagcgc cgctgcagtg acaggtagtc gccgaccggc cgcgtgcgct gtgcgtggtt
      661 cagcgtccac gtgcgcccgt gctccacttc gtacagcggg aacgcgccgc actcgaccgc
      721 gtagcgcgcg gcggtgagcc ccatgtcgtc cggcacgccc cagccgtcga ggcacgggat
      781 cagcagcgtg atgatgcgcg tgccgcgcag cgacttcgcc ttcttcacct tggcaagcag
      841 gtcgggcagg aagccgacgc tcgcggtcgc cacgtacggc acctcgtgcg cggccatgat
      901 ctcggtgagg ttcttcttgc gcgaggtctt gccggccggg gtggaaccgg tgctcgcgta
      961 atgcggcgtc gacgaggact tctgcgcgcc ggtgttcatg tagccctcgt tgtcgaggca
     1021 gaagtagatg aagtcctcgt tgcgctcggc cgccgatgac aggcactgga agccgatgtc
     1081 gtaggtgccg ccgtcgccgg cgagcacgat cacctgcgtg tcgcgcttgc cctgagcatc
     1141 gagcgcgcgc cgcaggccgc tcgccgcggc cgcgctcgct tcgagcgtgg gctggtagac
     1201 ggggatcttc agcgaactgt acggctgcgg gccggcgatg atcgccatgc acgacggcgg
     1261 aatcaccgcc atcgtgtccg gtcccatcgt cgcgaggatg tgtcgcaccg acagcgcctc
     1321 cacgcagccg gggcaggcgg cgtggccgga gcacagcatc gcatcgtcct gcagttcaag
     1381 cttgatcatg tcagcggacc cattcggatt cgggttgcgg ctcggagcct tgcgcgcggc
     1441 gcacgaactc ggcgatcttc tccggcgata cgttcacgcc gccgacaccg gcaaggtagc
     1501 cgtgc
//
LOCUS       DBNHBY010000796         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1821815, whole genome shotgun sequence.
ACCESSION   DBNHBY010000796 DBNHBY010000000
VERSION     DBNHBY010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1821815"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="AC16HB_18640"
     CDS             <1..549
                     /locus_tag="AC16HB_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5608865.1"
                     /translation="CILMGCGIPTTANYLIMVTVSAPTLVQLGVEPIVAHFFVFYYGV
                     LADITPPVCIAAYAAAGMAGSDPFRTGNTAFRLGLGKALVPFVFAFSPALLLVTKGFT
                     WSEFFVTFSGCALGIVALGASLSKFFLVEMRLWERWLAVFAAVLLIAPGLTPTLIGIV
                     LVAPIVLRHLLALRAAAPATAG"
     gene            595..1308
                     /locus_tag="AC16HB_18645"
     CDS             595..1308
                     /locus_tag="AC16HB_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5608866.1"
                     /translation="MNKVYPSAAAALAGVVKDGQTIAVGGFGLCGIPEALIAALRDSG
                     AKNLTCVSNNAGVDGFGLGLLLATRQVRKMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAERMRAGGAGIPAFFTATGYGTVIAEGKEVREFDGPWAGRHYVMERGIRADVSLVK
                     AWKADKQGNLVYRKTARNFNPVVAMCGKVTVAEVEQVVENGAIEPDLVHTPGIFVQRI
                     VLNATPEKRIEQRTVRAKA"
     gene            1318..1968
                     /locus_tag="AC16HB_18650"
     CDS             1318..1968
                     /locus_tag="AC16HB_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HIR5608867.1"
                     /translation="MPWTREEMAARAAQELRDGFYVNLGIGLPTLVANYIPRGMDVML
                     QSENGLLGIGPFPTEDEVDADLINAGKQLVTALPGSSYFSSADSFSMIRGGHINLAIL
                     GAMQVSEKGDLANWMIPGKLVKGMGGAMDLVAGVERVVVLMEHCAKNGEHKILQKCTL
                     PLTGVGVVNRIITDLGVFDVTPSGLKAIELAAGVTHEEVRVKTGCHVQVSHLPVTA"
     gene            1981..2925
                     /locus_tag="AC16HB_18655"
     CDS             1981..2925
                     /locus_tag="AC16HB_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5608868.1"
                     /translation="MSGTHNHLGEHRDGDSAYTHQSHSGGSRPLAVAVALTAGYAIVE
                     LLGGLWSGSLALLADSGHMATDAAALLFALAANYVARRPVSDRHSFGLARVEVIAAFV
                     NALAMLAVVTWIYVEAIDRIRNPVAVQGGEVFLIASIGLAVNIAVAWTLSRDRENVNT
                     RAAFIHVLGDLLGSVAAIVAGVVIWFGGPLISDPLLSMFVAALILRSTIGVLKETTLV
                     LLDSVPAGLDYAKVGDTLARVPGVLQVHDLHVWSMVPGRSALSAHVLVDDIEHWPVIL
                     RQARGLLATQFHIDHVTLQPEWLRREPPAAAMPIKRVS"
     gene            complement(2909..>3641)
                     /locus_tag="AC16HB_18660"
     CDS             complement(2909..>3641)
                     /locus_tag="AC16HB_18660"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HIR5608869.1"
                     /translation="AESLADTQRLRIVDALRGFAVAQMIVYHFIYDLNYFGFIRVRML
                     VDPQWIAWRTAIVTQFLLLVGVSLALRAVHKPSWRDFGRRWLQIAGAAALVSAGSWLV
                     FGPRFIWFGILHFVAAALLLARPLVGLGAWNLLLGGAALTLGLLYADPRFNPAPLNAL
                     GFITQKPNSEDYVPLFPWLGVVLVGTGLGALWARRGFAVAPLLAPLDRAPPRALVWLG
                     TWALTVYLLHQPVLMGLLLVVRKLA"
BASE COUNT          607 a         1173 c         1266 g          595 t
ORIGIN      
        1 tgcatcctga tgggctgcgg tattccgacc accgccaact acctgatcat ggtcacggtg
       61 tcggcgccga cactggtgca actcggcgtg gagccgatcg tcgcgcactt cttcgtgttc
      121 tattacggcg tgctcgcgga catcacgccg ccggtgtgca tcgccgcgta tgcggccgcg
      181 ggcatggcgg gcagcgatcc gttccgcacc ggcaacaccg cgttccggct cgggctgggc
      241 aaggcgctgg tgccgttcgt gttcgcgttt tcgcccgcgc tgctgctcgt caccaagggg
      301 ttcacctgga gcgaattctt cgtcaccttc tccggctgcg cgctcggcat cgtcgcgctg
      361 ggggcgtcgt tgtcgaaatt cttcctggtc gagatgcggc tgtgggaacg ctggctggcg
      421 gtgttcgccg ctgtgctgct gatcgcgccg gggctcactc cgacgctgat cggcatcgtg
      481 ctggtggcgc cgatcgtgct gcgccatctg cttgcactgc gagcggccgc accggcgacg
      541 gcgggctgag caacgtcgct agaatcgcag cgatgtgttg agaaaaggat cgacatgaac
      601 aaggtctacc catcggctgc ggcggcgctc gctggcgtgg tcaaggacgg gcaaacgatt
      661 gccgtcggcg gcttcggcct gtgcgggatt cccgaggcgc tgatcgcggc gctgcgcgac
      721 agtggcgcca agaacctgac ctgcgtcagc aacaacgccg gcgtcgatgg attcggcctc
      781 gggctgctgc tggcgacccg gcaggtcaga aagatgatct cttcgtacgt cggcgagaac
      841 aaggaattcg agcgccagta tctggccggc gaactcgaac tcgagttcac cccgcagggt
      901 acgctcgccg agcgcatgcg cgccggcggc gcgggaatcc cggcgttctt cacagcgacg
      961 ggttatggca cggtgatcgc cgaaggcaag gaggtgcgcg agttcgacgg tccgtgggcc
     1021 ggccgccact acgtaatgga gcgcggcatc cgcgccgacg tctcgctggt caaggcgtgg
     1081 aaagccgaca agcagggcaa tctcgtctat cgcaagaccg cgcgcaactt caatccggtg
     1141 gtggcgatgt gcggcaaggt gacggtcgcc gaggtcgagc aggtcgtcga gaacggcgcg
     1201 atcgagcccg accttgtgca tacgcccggc atcttcgtac agcggatcgt gctgaacgcg
     1261 acacccgaga aacgcatcga gcagcgcacc gtgcgcgcaa aggcttagga ggaccccatg
     1321 ccctggaccc gtgaagagat ggcggcgcgc gccgcgcagg aactgcgcga cggcttctat
     1381 gtgaacctcg gcatcggtct gccgacgctg gtggcgaact acattccgcg cggcatggac
     1441 gtgatgctgc agtcggaaaa cggcctgctc ggcatcggcc cgtttccgac cgaagacgag
     1501 gtcgacgcgg atctcatcaa cgcgggcaag cagcttgtca ctgcgctgcc gggctcgagc
     1561 tatttctcga gcgctgattc gttctcgatg atccgcggcg gccatatcaa ccttgccatc
     1621 cttggcgcga tgcaggtgag cgagaagggc gatctggcca actggatgat cccgggcaaa
     1681 ctggtcaagg gcatgggcgg cgcaatggac ttggtggctg gcgtcgagcg cgtggtggtg
     1741 ctgatggagc actgcgcgaa gaacggcgag cacaaaatcc tgcagaagtg cacgcttccg
     1801 ctgaccggcg tcggcgtggt caaccgcatc atcaccgacc tcggcgtttt cgacgtgacg
     1861 ccgagtggac tgaaggcgat cgagctggcg gcgggcgtca cgcacgagga agtgcgggtg
     1921 aagaccggct gccacgtgca ggtatcgcat ctgccggtga ccgcgtagac atcgagccgc
     1981 atgagcggca cgcacaacca cctcggcgag caccgcgacg gcgacagcgc ctacacgcac
     2041 cagtcgcatt cgggcggctc gcggccgctc gcggtcgcgg tggcgctcac cgcgggctat
     2101 gcgatcgtcg agctgctcgg cggcctgtgg tccggctcgc tggcgctgct ggcggactcc
     2161 ggccatatgg cgacggacgc ggcggcgctg ttgtttgcgc tcgccgccaa ctacgttgca
     2221 cggcggcccg tgtcggaccg gcattcgttc ggactcgcgc gcgtcgaggt gatcgccgcg
     2281 tttgtcaatg cactggcgat gctggcggtg gtcacgtgga tctacgtcga ggcgatcgac
     2341 cgcatccgca atccggtcgc ggtccagggc ggcgaggtgt tcctgatcgc ctcgatcggc
     2401 ctggcggtca acattgcggt tgcctggacg ctgtcgcgcg atcgcgagaa cgtgaacacc
     2461 cgcgcggcgt tcattcatgt cctgggcgac ctgctcggat cggtcgccgc gatcgtcgcc
     2521 ggcgtggtca tctggttcgg cggcccgctg atctccgacc cgctgctgtc aatgttcgtc
     2581 gccgcgctga tcctgcgctc gaccatcggc gtgctgaagg aaaccacgct cgtgctgctc
     2641 gacagcgtgc cggccggcct cgactacgcg aaggttggcg acacgctggc gcgcgtgccc
     2701 ggtgtgttgc aggtgcacga cctgcacgtt tggtcgatgg tgcccgggcg cagcgcgctg
     2761 tcggcgcacg tgctggtcga cgacatcgag cactggccgg tgatcctgcg gcaggcgcgc
     2821 gggctgctcg cgacacagtt ccacatcgac cacgtcacgc tgcagcccga atggctgcgg
     2881 cgcgaaccgc cagccgcggc gatgccgatc aagcgagttt cctgaccacc aacagcaatc
     2941 ccatcaagac cggctggtgc agcaggtaga cggtcagcgc ccaggtgccg agccagacca
     3001 gcgcgcgcgg cggtgcgcgg tcgagcggcg cgaggagcgg cgccaccgca aagccacggc
     3061 gtgcccacag cgcgcccagc ccggtgccga ccagcaccac gcccagccat ggaaacaacg
     3121 gcacgtagtc ctcgctgttc ggcttctgcg tgatgaagcc gagtgcattg agcggcgccg
     3181 ggttgaaccg cggatccgca tagagcaggc cgagcgtgag cgcggcgccg ccgagcagca
     3241 ggttccacgc gccgagcccc accagcgggc gcgcgagcag cagcgccgcc gcgacgaagt
     3301 gcaggatgcc gaaccagatg aagcgcggcc cgaacacgag ccagctgccg gccgacacga
     3361 gcgcggccgc accggcgatc tgcagccagc gccggccgaa gtcgcgccac gacggcttgt
     3421 gcacggcgcg cagtgcgagg ctcacgccga ccagcagcag gaactgggtg acgatcgcgg
     3481 tgcgccaggc aatccactgc ggatcgacca gcatccgcac gcggatgaaa ccgaagtaat
     3541 tgaggtcgta aatgaagtgg tagacgatca tctgcgcgac tgcgaagccg cgcagcgcgt
     3601 cgacgatccg gaggcgttgc gtgtctgcca aagattccgc t
//
LOCUS       DBNHBY010000797         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1077122, whole genome shotgun sequence.
ACCESSION   DBNHBY010000797 DBNHBY010000000
VERSION     DBNHBY010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1077122"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1394
                     /gene="oxlT"
                     /locus_tag="AC16HB_18665"
     CDS             105..1394
                     /gene="oxlT"
                     /locus_tag="AC16HB_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0019531 - oxalate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA];
                     GO_process: GO:0019532 - oxalate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HIR5608870.1"
                     /translation="MSSTASVSTTAQATGFGRWWQLLIGVMCMSMIANLQYGWTLFVN
                     PINEQYHWGKAAIQVAFTIFVLTETWLVPLEGWLVDKYGPRPMMLAGGVLVGISWMLN
                     SVADSLWLLYVAAAIGGVGAGGVYGTCVGNALKWFPDRRGLAAGLTAMGFGAGSALTV
                     IPIATMITSSGYQATFLYFGVAQGLIVFVLAWFMRKPDPAFRVAPVHKRAAAAPVAAP
                     RRDYTPLQMITTPVFWVMYAMFVMMAAGGLMATAQLGPIAKDFKIADVPVSILGLTLP
                     ALTFALSIDRVLNGVTRPIFGWVSDRIGREQTMFFAFFLEGIGILLLGKYGHNPLLFV
                     ILTGLVFFAWGEIYSLFPATCGDTYGSRYAAANAGLLYTAKGTASLLVPLSSLLAASG
                     GWHLVFWVASALNIVAALMALTLLRPMRTRFREGNGT"
     gene            complement(1402..>1706)
                     /locus_tag="AC16HB_18670"
     CDS             complement(1402..>1706)
                     /locus_tag="AC16HB_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR5608871.1"
                     /translation="IESYIDEMAGVVESAVIGLPHPDFGEAVAAVVVGAPGATVDGDA
                     IIARLKEKIANFKVPKRVWVVAELPRNAMGKVQKNLLRERYANVFAAPAQPSAATR"
BASE COUNT          255 a          562 c          529 g          360 t
ORIGIN      
        1 agatcccggt cctcgtctat cgctagctga ggcttcgtcc gccgtttcct cccgaccaac
       61 ctagcccgag ccatgccgct cggtccagca ggagcctcca tcgaatgagt tcaaccgcat
      121 ccgtctcgac caccgcgcaa gccactggtt tcggccgctg gtggcaacta ctgattggcg
      181 tcatgtgcat gtcgatgatc gccaaccttc agtacggatg gacgctgttc gtcaatccta
      241 tcaacgagca gtatcactgg ggcaaggctg cgatccaagt tgcgttcacg atcttcgttc
      301 tgactgagac gtggctagtg ccgctcgagg gctggctggt cgacaagtac ggcccgcggc
      361 cgatgatgct ggccggcggc gtgctggtcg gcatctcgtg gatgctgaac tcggtcgcag
      421 attcgctctg gctgctgtat gtcgccgcag cgatcggcgg agtgggcgcg ggcggcgtat
      481 acggaacctg cgtcggcaat gcgctgaagt ggtttcccga ccggcgcggc ctcgccgcgg
      541 gactgacggc gatgggattc ggcgccggct ccgcgctgac cgtcattcca attgccacga
      601 tgatcacttc gagcggttat caggcgacgt ttctctattt cggcgtcgcc cagggattga
      661 tcgtgttcgt gctcgcatgg tttatgcgca agccagatcc ggcgttccgc gtcgctccag
      721 tgcacaaacg ggccgcggcc gccccggtcg cggcgccgcg gcgcgactac acgccactgc
      781 agatgatcac gacgccggtg ttctgggtta tgtatgcgat gttcgtcatg atggccgctg
      841 gcggcctgat ggcgacggcc cagctcggcc ccattgcgaa ggacttcaag attgccgatg
      901 taccagtcag catcctcggg ctgacgcttc cggcgctgac ttttgcgcta tcgatcgacc
      961 gcgttctcaa cggagtcacg cgtccgatct tcggctgggt ctctgacagg atcggtcgcg
     1021 agcagacgat gttcttcgcc ttctttctcg aaggtatcgg catcctcctg ctgggcaagt
     1081 acggccacaa cccgctgctg ttcgtgatcc tcaccgggct cgtgttcttc gcttggggcg
     1141 agatctacag cttgtttcct gccacctgtg gcgacacgta tggctcccgc tacgcggcgg
     1201 ccaatgccgg cctgctgtac acggccaagg gcacggcgtc gctgctcgtc ccgctgtcga
     1261 gccttttggc tgccagcggc ggctggcatc tggtcttctg ggtggcgagc gcgctgaaca
     1321 ttgtggcggc gctgatggcg ttgacgctcc tcagaccgat gcgcacgcgc ttccgcgagg
     1381 gcaacggtac ctgattgccg ctcagcgagt cgccgcgctg ggctgcgccg gcgccgcaaa
     1441 cacattggcg taccgttcgc gcagcaggtt cttctgcacc ttgcccatcg cattgcgcgg
     1501 cagttcggcc acgacccaca cgcgcttggg caccttgaag ttggcgatct tctccttcag
     1561 ccgcgcgatg atcgcgtcac cgtcgaccgt cgcgcccggc gcaccgacga cgaccgccgc
     1621 aaccgcttcg ccgaagtccg gatgcggcag gccgatcacc gccgactcga ccacgccggc
     1681 catctcgtcg atgtagctct cgatct
//
LOCUS       DBNHBY010000798         2946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_739670, whole genome shotgun sequence.
ACCESSION   DBNHBY010000798 DBNHBY010000000
VERSION     DBNHBY010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_739670"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16HB_18675"
     CDS             <1..464
                     /locus_tag="AC16HB_18675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR5608872.1"
                     /translation="PSAGPSATAELKPTQGNNATGTVQFMQQGNKVAVMVSVRGLPPN
                     SEHGFHVHEKGDCSAPDGMSAGGHFNPGSRPHAHFEQGERHAGDLPNLRADANGYANY
                     RWETDALSVGGGANSVIGRAVIVHRDPDDYKTQPAGNSGPRVACGVIAAAR"
     gene            598..2115
                     /locus_tag="AC16HB_18680"
     CDS             598..2115
                     /locus_tag="AC16HB_18680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608873.1"
                     /translation="MNASTLRRPGAVAVIVLVIAAVAAAWWWRTAADKEAPSYRFARV
                     EKGPITATVSASGTLNPVTSVQVGTQVSGQIKELFVDFNSVVKQGQLIARIDPETFTY
                     RVRQAEADLEAARAAVGRAQVAMLNARRDLDRSRELVEKNFASPADVDAKQAAYDLGL
                     ADVKNSQAVLRQREAALASARVDLTRTEIRAPVNGVVIKRSVDVGQTVAASLQAPELF
                     VIAKDLSDMQVETSIDEADVGRMRIGQRASFTVDAFPGRTFAGSVTQIRKAAQNVQNV
                     VTYTVLVSASNEGGQLMPGMTANVRIVTDTRDNVLKIANAALRFRPAGVAVNDSGAAK
                     AGDVAKTPGDATGGPAATGGGQLQQFRERLVTELKLDADQQAKLEPIFAALRTRFASL
                     RDLPEEQRARAAQAARAELRAKVEEILKPEQKPRYAELAAEIAGRSSQPTRGRVWVID
                     NGRPKVIDVRIGLTDGTATEVSGDGINEGTEVIVGLQGAAGSAPAQKGSPPRMFF"
     gene            2123..2866
                     /locus_tag="AC16HB_18685"
     CDS             2123..2866
                     /locus_tag="AC16HB_18685"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5608874.1"
                     /translation="MAALIEVTDLRKDYVLGDTTVHALAGVTLAIDAGEFVAVMGPSG
                     SGKSTFMNMIGCLDRPTSGGYHLAGEDVAHMEADELAAIRNRRIGFVFQQFNLLARTS
                     ALENVELPLVYAGLAAKERHARAAAKLALVGLGARLDHQPSQLSGGQQQRVAIARALV
                     NDPRLILADEPTGALDSRTSIEIMALLQQLNRSGMTIVVVTHERDIAAYASRLIVFRD
                     GQVIRDERQAPSDAAADLPALVAQPVAAE"
BASE COUNT          494 a          981 c         1061 g          410 t
ORIGIN      
        1 gcccgtcagc gggcccctcg gccacagcgg agctgaagcc gacccagggc aacaacgcga
       61 cgggcaccgt gcagttcatg cagcagggca acaaggtagc cgtcatggtc agcgtgcggg
      121 gattgccacc gaacagcgag catggctttc acgtgcacga aaagggcgat tgctccgcgc
      181 ccgacggcat gagcgccggc gggcatttca atccgggcag caggccgcac gcgcacttcg
      241 agcagggtga gcgccacgcg ggcgatctgc cgaacctgcg cgcggacgcc aacggctacg
      301 ccaactacag gtgggaaacc gacgcgctct ccgtcggtgg cggcgccaac agcgtgatcg
      361 gccgtgcggt gatcgtgcac cgcgaccccg acgactacaa gacgcagccg gccggcaact
      421 cgggcccgcg cgtggcgtgc ggcgtgatcg ctgcggcgag atagcgcgcc gatcgtggta
      481 ttgcgcggcg ccggcggcgt gttgccgact gcgccggcgt tgtatcggct cgtatccgtg
      541 ctcggaagcg cgggagtggc cgcatagaat cggcgcgtcg cgtcaacgag ttcccgaatg
      601 aacgcatcca ccctgcgccg gcccggcgca gtcgccgtga tcgtgctcgt catcgccgcg
      661 gtcgcggccg catggtggtg gcgtacggcg gcggacaagg aagcgccgtc ctatcggttt
      721 gccagggtcg agaaggggcc gatcaccgcg acggtgtcgg cgtcgggcac gctgaatccg
      781 gtcacctcgg tgcaggtcgg cacgcaggtg tcggggcaga tcaaggaact gttcgtcgac
      841 ttcaactcgg tggtcaagca gggccagctg atcgcgcgca tcgacccgga gaccttcacc
      901 tatcgcgtgc gccaggccga ggccgatctc gaggcggcgc gcgccgccgt cggtcgcgcg
      961 caggtcgcga tgctcaatgc caggcgcgac ctggaccgca gccgcgagct ggtggagaag
     1021 aacttcgctt cgccggccga tgtcgatgcc aagcaggcgg cgtacgacct cgggcttgcc
     1081 gacgtgaaga actcgcaggc ggtgttgcgc cagcgcgaag cggcgctcgc ctcggcgcgc
     1141 gtcgatctca cgcgcaccga gatccgcgca ccggtcaacg gcgtggtgat caagcgcagc
     1201 gtcgatgtcg ggcagaccgt ggccgccagc ctgcaggcgc cggaactgtt cgtgatcgcg
     1261 aaggacctgt ccgacatgca ggtcgagacc tcgatcgacg aagcggatgt cggccgcatg
     1321 cgcatcggcc agcgtgcgag cttcaccgtc gatgcgtttc cgggccgcac gttcgcgggc
     1381 agcgtgacgc agatccgcaa ggcggcgcag aacgtgcaga acgtcgtcac ctacacggtg
     1441 ctggtcagcg ccagcaacga aggcggacag ttgatgccgg gcatgaccgc caacgtgcgc
     1501 atcgtcaccg acacccgcga caacgtgttg aagattgcca acgccgcgct gcgattccgc
     1561 ccggccggcg tcgcggtcaa cgattcgggt gccgcgaaag ccggcgatgt ggcaaagacg
     1621 cctggcgacg cgacgggcgg accggccgcc acgggcggcg ggcagttgca gcagtttcgc
     1681 gaacggctgg tgacggagct caagctcgac gctgaccagc aggcgaagct cgagccgatc
     1741 ttcgccgcac tgcgcacgcg ctttgcatcg ctgcgcgacc tgcccgagga gcagcgcgcc
     1801 cgggccgcgc aggcggcgcg cgcggaactg cgcgcgaagg tcgaggagat cctgaagccc
     1861 gagcagaagc cgcgctacgc ggaactcgcg gccgagatcg ccggtcgcag cagtcagccc
     1921 acgcgcggcc gcgtatgggt gatcgacaac ggcaggccga aggtaatcga cgtgcgcatc
     1981 ggacttacgg acggcaccgc gaccgaggtc agcggcgacg gcatcaacga gggcaccgag
     2041 gtgatcgtcg gcctgcaggg tgcggcgggc agcgcgccgg cacagaaggg ctcgccgccg
     2101 cggatgttct tctaggtcga ccatggccgc gttgatcgaa gtcaccgacc tgcgcaagga
     2161 ctacgtgctt ggcgatacga ccgtgcacgc gctggcgggc gtgacgctgg caatcgacgc
     2221 cggggagttc gtcgcggtga tggggccgtc gggctcgggc aagtcgacct tcatgaacat
     2281 gatcggctgc ctcgaccggc cgacttcggg cggctaccac ctcgccggcg aggatgtcgc
     2341 gcacatggaa gcggatgaac tcgcagcgat ccgcaaccgt cgcatcggct tcgtgttcca
     2401 gcagttcaac ctgctggcgc gcaccagcgc gctggaaaac gtcgagctgc ccctggtgta
     2461 tgcgggcctg gcggccaagg agcgccacgc ccgcgcggcg gccaagctgg cgctggtcgg
     2521 gttgggcgcg cgactcgatc accagccgtc gcagttgtcg ggcgggcagc agcagcgcgt
     2581 cgcgatcgcg cgcgcgctcg tcaacgaccc gcggctcatc ctcgccgatg agccgaccgg
     2641 cgcgctcgat tcgcgcacca gcatcgagat catggcgctg ctgcagcagc tgaatcgcag
     2701 cggcatgacg atcgtcgtcg tcacgcacga gcgcgacatt gcggcctacg cgagccgact
     2761 gatcgtgttc cgcgacggcc aggtgatccg cgacgagcgc caggcgccga gcgatgcggc
     2821 ggccgatctc cccgcgctcg tcgcgcagcc ggtggcggcg gagtagggtc ggctcatgaa
     2881 cctgctcgcc gtgctccgcg tcgcgctgaa ggcgctgcgc gtgaacaagc tgcgctcgag
     2941 cctgac
//
LOCUS       DBNHBY010000799         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_198495, whole genome shotgun sequence.
ACCESSION   DBNHBY010000799 DBNHBY010000000
VERSION     DBNHBY010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_198495"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16HB_18690"
     CDS             <1..917
                     /locus_tag="AC16HB_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5608875.1"
                     /translation="FQASGRIVARLVDVGSHVKRGQPLARLDPAQETLHVVAATADVD
                     AAKSRVEQNRVDLQRAEQLLARKFASQAEVDQQRLALEQAQSQLKAALAQQQITINQR
                     GYTTLAADRDGVVTAISAEAGQVVSAGQPIVTVAADGEREVVVSIPESRVDELRQAKS
                     LQVSVWAQPGKSYQGALRELAPDTDSVTRTYSARIAVKNPDGALRLGMTASVFAPDVE
                     GSRAIRLPLTAIYDKDGQPQVWVVDAKTSVVATRAVVLGAAQNDHVLITRGLEGGETV
                     VTAGVHMLHGGQKVKVVESTLVAVGAGK"
     gene            914..>2663
                     /locus_tag="AC16HB_18695"
     CDS             914..>2663
                     /locus_tag="AC16HB_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5608876.1"
                     /translation="MTRINLSEWALKHPQMIVFLLLLLSIAGVLAYGQLGQKEDPEFT
                     IKAMLVQAHWPGASAQQMADQVTDKLERKLQEVAEIDYTVSYAKPGVTQITISLREDT
                     APQAVPQVWYQVRKKLGDIQHTLPQGVRGPFFNDEFGDTFGNLYAITGDGFGYAELRD
                     FADAARNEFLRVADVNKVDLTGAQDEKIYVEASNAKLASLGIDPSLIASTLASANTVA
                     AAGTVQTTNEQVRLTVSGEFDSVDSIRNIGIRAGERMFRLGDIAEVKRGVIDPAVSKM
                     RFGGHDAIGLAVSMRKGGDVIWLGEQLNATVKRVQASLPVGVEIHAVSDQPRVVNESV
                     HEFKKSLAEAVIIVLAVSFFSLGVRTGLVVALCIPLVLALTFLTMYFLGIELQRISLG
                     ALIIALGLLVDDAIIAVEMMALKLEQGWDKFRAATYAYTATAFPMLTGTLITAAGFLP
                     VGFAKSGSGEYVFSLFQVVGVSLMLSWIVAVLFTPYIGYKLLREDHAASHDEDAVYQR
                     GFYLRFRRVVDACLVHRKWVIGVTVAAFVGAIALFRLVPQQFFPASDRPELMIDLWLP
                     QAATFDASLREVQALEA"
BASE COUNT          457 a          867 c          908 g          431 t
ORIGIN      
        1 gcttccaggc ctccggccgg atcgtcgcgc ggctggtcga tgtgggcagc cacgtaaagc
       61 gcggccagcc gctggcgcgg ctcgacccgg cgcaggaaac gctacacgtc gtcgccgcga
      121 ccgccgatgt cgatgcggcg aagagccgcg tcgagcagaa ccgcgtcgat ctgcagcgcg
      181 ccgagcagtt gctggcgcgc aaatttgcct cgcaggccga ggtcgaccag cagcggctgg
      241 cgctcgaaca ggcccaatcg cagctgaagg cggcgctggc gcagcagcag atcacgatca
      301 accagcgcgg ctacaccacg ctcgccgccg atcgagacgg cgtcgtcacc gccatcagcg
      361 cggaggccgg acaagtcgtc tccgcgggtc agccgatcgt gacggtcgcg gccgacggcg
      421 agcgcgaagt ggtcgtcagc atccccgagt cgcgcgtcga cgaactgcgg caggcaaagt
      481 cgctgcaggt ttctgtctgg gcgcagcccg ggaagtccta tcagggcgcg ctgcgcgaac
      541 tcgcgcccga taccgacagc gtgacccgca cctactcggc acgtatcgcg gtgaagaacc
      601 ccgatggcgc gctgcggctc ggcatgacgg cgtcggtgtt cgcgcccgat gtcgagggaa
      661 gccgcgcgat ccggctgcca ctgacggcga tctatgacaa ggacggccag ccgcaggttt
      721 gggtggtcga cgccaagact tcggtggtcg ccacgcgcgc ggtcgtgctc ggcgcggcgc
      781 agaacgacca cgtgctgatc acccggggac tcgaaggcgg tgagaccgtg gtcaccgccg
      841 gtgtgcacat gctgcacggc ggccagaagg tcaaggtcgt ggaaagcacg ctcgtcgcgg
      901 tcggagccgg gaaatgacca ggatcaacct ctccgaatgg gcgctcaagc acccgcagat
      961 gatcgtcttc ctgctgctgc tgttgagcat tgccggcgtg ctcgcctacg gccagctcgg
     1021 ccagaaggag gacccggaat tcaccatcaa ggcgatgctg gtgcaggcgc attggcccgg
     1081 cgcctcggcg cagcagatgg ccgaccaggt gaccgacaag ctcgaacgca aactgcagga
     1141 agtcgctgaa atcgactaca cggtgagcta cgccaagccg ggggtcaccc agatcacgat
     1201 cagcctgcgc gaagacacgg ccccgcaggc ggtgccgcag gtctggtacc aggtacgcaa
     1261 gaagctcggt gatatccagc acacgctgcc gcagggcgtg cgcgggccgt tcttcaacga
     1321 cgagttcggc gacaccttcg gcaacctgta cgcgatcacg ggcgacgggt tcggctacgc
     1381 cgagttgcgc gacttcgccg acgccgcgcg caacgagttc ctgcgcgtag cggacgtcaa
     1441 caaggtcgat ctgaccggcg cgcaggacga gaagatctac gtcgaggcgt ccaacgccaa
     1501 gctggcatcg ctcggcatcg atccttcgct gatcgcgagc acgctcgcat cggccaacac
     1561 cgtcgccgcc gccggcaccg tccagacgac aaacgagcag gtgcggctga cggtgagcgg
     1621 cgagttcgat tcggtcgaca gcatccgcaa catcggcatc cgcgccggcg aacgcatgtt
     1681 ccgccttggc gacatcgccg aggtcaagcg cggtgtgatc gatccggccg tgtcgaagat
     1741 gcgcttcggc gggcacgatg cgatcggact cgcggtcagc atgcgcaagg gcggcgatgt
     1801 gatctggctc ggcgagcaac tgaacgcgac cgtcaagcgc gtgcaggcga gcctcccggt
     1861 gggagtggag atccatgcgg tgtccgacca gccgcgcgtg gtcaatgaat cggtgcacga
     1921 gttcaagaag agcctcgcgg aggcggtgat catcgtgctc gcggtcagct tcttctcact
     1981 cggcgtgcgc acggggctcg tcgttgcgct gtgtatcccg ctggtgctgg cgctcacctt
     2041 cctgacgatg tacttcctgg gaatcgaact gcaacgcatc tcgctcggcg cgctgatcat
     2101 cgcgctgggt ttgctggtcg acgacgcgat catcgcggtg gagatgatgg cgctgaagct
     2161 cgagcagggc tgggacaagt tccgcgccgc gacctacgcc tacaccgcga ccgcgtttcc
     2221 gatgctcacc ggcacgctga tcaccgccgc gggcttcctg ccggtgggtt ttgccaagtc
     2281 gggctcgggc gagtacgtgt tctcgctgtt ccaggtggtc ggcgtttcgc tgatgctgtc
     2341 gtggatcgtc gcggtgctgt tcacgccgta catcggctac aagctgctgc gcgaagacca
     2401 cgcggcaagc cacgacgagg atgccgtcta ccagcgcggc ttctacctgc ggttccggcg
     2461 cgtcgtcgat gcctgcctcg tgcatcgcaa atgggtgatc ggcgtcacgg tcgcggcgtt
     2521 cgtcggcgcg atcgcgctgt tccgcctggt gccgcagcag ttcttcccgg cttccgaccg
     2581 ccccgaactg atgatcgacc tgtggctgcc gcaggcggcc acgttcgacg caagcctgcg
     2641 tgaggtgcag gcacttgaag cga
//
LOCUS       DBNHBY010000800         2334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_739962, whole genome shotgun sequence.
ACCESSION   DBNHBY010000800 DBNHBY010000000
VERSION     DBNHBY010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_739962"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16HB_18700"
     CDS             complement(<1..386)
                     /locus_tag="AC16HB_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608877.1"
                     /translation="MKLTTLKTLAVSVGVVAALGCGAAFAQDKGKAAAKAPAKAAAGG
                     GEQFFPALPYRTGAYAPNGVPWANGYADYLKLVNARDGGINGVKITYEECETGYATDR
                     GVECYERLKGKGPTGATLFQPLSTGIT"
     gene            complement(447..1511)
                     /locus_tag="AC16HB_18705"
     CDS             complement(447..1511)
                     /locus_tag="AC16HB_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5608878.1"
                     /translation="MLYRENGQFKTTYRSDQQILPILQDRWFVWSLVALAFLALPLVA
                     SDYLFRALIVPFLILTIAAIGLNILVGYCGQISVGMAAFMAVGAYAAYNLVIRVPGMN
                     FLIVLLLAGLVAALVGIVFGVPSLRIKGLYLAVATLAAQFFIDWMFARVKWFTNYSPS
                     GSVSPAPVNLFGWTIERPVDKYWFVLGVLVVMTLVAKNLVRSHIGRAWMAIRDMDVAA
                     EVIGIRPLYAKLSAFAVSSFFAGVAGALWGFVHLGAWEPLAFNLNVSFNLLFMIIIGG
                     MGSILGSYFGAAFIVMLPLLLNQLPGWFGIELSTALLSHLEAMIFGALIVLFLIVEPH
                     GLARLWAIGKEKLRLWPFPH"
     gene            complement(1609..>2334)
                     /locus_tag="AC16HB_18710"
     CDS             complement(1609..>2334)
                     /locus_tag="AC16HB_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5608879.1"
                     /translation="LALAIMIAFAWLVERLVLRHLVNQEAVVLLMATLGVSYLIDGVG
                     QTIWGSDIYEINLGLPKTPTMILDGIFQGGILINQEDFAAALVAALLVASLALFFQKT
                     ATGRALRAVADDHQAAQSIGIPLNRIWVIVWAVAGIVALVAGMIWGSKLGVQFSLSQV
                     ALKALPVVILGGLTSVPGAILGGLIIGVGEKFAEVFLGPELVKAFDLAGGGIENWFAY
                     VLALAFLLVRPEGLFGEKHIDRV"
BASE COUNT          486 a          795 c          683 g          370 t
ORIGIN      
        1 gtgatacccg tggacagcgg ctggaacaaa gtcgcaccgg ttggtccctt gcccttcagt
       61 cgctcatagc actcgacgcc gcggtcggtc gcgtagccgg tctcgcattc ctcgtaggtg
      121 atcttgacgc cgttgatgcc accgtcgcgg gcattgacca gcttcaggta atccgcgtat
      181 ccgtttgccc agggcacacc gttgggcgca taggcaccgg tccgataagg cagggcggga
      241 aagaactgct cgccgcctcc agctgcggcc ttggccggcg ctttggcggc cgccttgccc
      301 ttgtcctggg cgaatgccgc gccgcacccg agtgccgcaa cgacgcccac cgaaaccgcg
      361 agggtcttga gtgtcgtgag tttcatcttg ccagtctcct ttcgaaagtc attgcccggg
      421 gtcggcctgc ccgagcgccc agaaaatcag tgcgggaacg gccacagtcg cagcttctcc
      481 ttgccgattg cccatagtcg cgccaggccg tgcggctcca caatcaggaa caacacgatc
      541 agagcgccga aaatcatggc ctccagatgc gatagcagcg ccgtcgacag ttcgattccg
      601 aaccagcccg gcagttggtt cagcagcagc ggcagcatca cgatgaacgc cgcgccgaaa
      661 tagctgccca ggatcgagcc catgccgccg ataatgatca tgaagagcag gttgaagctc
      721 acattgaggt tgaacgccag cggttcccac gcgcccaggt ggacgaatcc ccacaacgcg
      781 cccgcgacgc ccgcgaagaa cgagctgacc gcgaatgccg acaactttgc atacaggggt
      841 cgaatcccga tcacttccgc cgccacgtcc atgtcgcgga tcgccatcca cgcgcggccg
      901 atatgcgagc gcaccagatt cttggccacc agcgtcatca ccacgagaac gccgagcacg
      961 aaccagtact tgtcgaccgg ccgctcgatg gtccagccga acaggttcac cggcgccggc
     1021 gacaccgagc cagacggcga atagttggtg aaccacttca cgcgcgcgaa catccagtcg
     1081 atgaagaact gcgccgccag cgtggcgact gcgagataca ggcccttgat gcgcagactc
     1141 ggcacgccga acacgatgcc gaccagtgcc gcgaccaggc ctgccagcag cagaacgatc
     1201 aagaagttca tccccggcac gcggatcacc aggttatagg ctgcgtacgc acccaccgcc
     1261 atgaatgcgg ccattccgac cgagatctgc ccgcagtagc ccaccaggat gttcaacccg
     1321 atggccgcga tggtcaggat caggaatggg acgatcagcg cgcggaacag gtagtcgctc
     1381 gccaccagcg gcagtgccag aaatgcgagc gccaccagtg accacacgaa ccagcgatcc
     1441 tgcaggatcg gcaggatctg ctgatccgac cggtaagtcg tcttgaactg gccgttctcg
     1501 cggtaaagca tcgttgttca gtccgtcgat gtgctcttgt ttccggccac tcgtttcacg
     1561 cgctcgactt gctacgcagg tcagcgtcca gcgaagccac gtcaatcgct acacgcgatc
     1621 gatgtgcttt tctccaaaca acccctccgg ccgcaccagc aggaatgcca gcgcgagcac
     1681 ataggcaaac cagttctcga tgccgccgcc agccaggtcg aaagccttga ccagttcagg
     1741 accgaggaac acttccgcaa acttctcacc gacgccgatg atcagcccgc cgagaatcgc
     1801 gcccggaacc gacgtgagtc cgccgagaat caccaccggc agcgctttca gcgcgacctg
     1861 cgacagcgag aactgcacgc cgagcttgga gccccagatc attccggcca cgagcgcgac
     1921 gatgccggcc accgcccaga cgatgaccca gatccggttc aacggaatgc cgatcgactg
     1981 cgccgcctgg tgatcgtcgg cgaccgcgcg cagcgcgcgt cccgtcgcgg tcttctggaa
     2041 gaacagcgcg agcgatgcca cgagcagcgc ggcgaccagc gccgcggcga agtcctcctg
     2101 attgatcagg atgccgccct ggaagatgcc gtcgagaatc atcgtcggcg tcttgggcag
     2161 gccgaggttg atctcgtaga tgtcgctccc ccagatcgtc tgcccgacgc cgtcgatgag
     2221 gtaggaaacg ccgagcgtgg ccatcagcag caccacggcc tcctggttga cgaggtgccg
     2281 cagcaccagc cgctccacca gccaggcgaa cgcgatcatg atcgccagcg ccag
//
LOCUS       DBNHBY010000801         1606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_130998, whole genome shotgun sequence.
ACCESSION   DBNHBY010000801 DBNHBY010000000
VERSION     DBNHBY010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_130998"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16HB_18715"
     CDS             complement(<1..544)
                     /locus_tag="AC16HB_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR5608880.1"
                     /translation="MTVPRYPGFDTLVLHAGQRPDTATGARAVPIYASTSFCFDDSDH
                     AAALFNMERAGHVYSRISNPTVAVLEERMAALENGVGAIAAASGQAALHLAIVTLMGA
                     GSHIVASRALYGGSHNLLAYTLPRFGVETTFVDPRNLDAWRAAIRPNTRLLFGETLGN
                     PGLDVLDVPRVAELAHSHGLP"
     gene            629..1573
                     /gene="pip"
                     /locus_tag="AC16HB_18720"
     CDS             629..1573
                     /gene="pip"
                     /locus_tag="AC16HB_18720"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIR5608881.1"
                     /translation="MRADLYPPLEPYDSGMLALDDVHTMFWEACGNPHGIPVLFLHGG
                     PGGGCSPEHRRFFDPDVFRVVLFDQRGAGNSTPLGDVTNNTTQHLVRDIELLRSYLGF
                     GKALVFGGSWGSTLALAYGQTHPDRCLGFVLRGVFLGRASEIEWFLYGLRHIFPEAWR
                     AFAEMLPEEERGDLLRGYLKRLFDPDPRVHVPFARAWSEYEGLCSTLLPNPDLVRHFA
                     DEAVGLARLEAHYFAHQCFLAPNQLLANLYRVRHVPVSIVQGRYDMVCPIVSADDLAR
                     AWPGARYVVVNDAGHSVWEPAIRSAVMREVELFKQALG"
BASE COUNT          235 a          527 c          565 g          279 t
ORIGIN      
        1 acggcagccc atgcgagtgg gcgagctccg ccacgcgcgg gacgtcgagc acgtcgagcc
       61 cggggttgcc gagcgtctcg ccgaacagga gccgcgtgtt cggccggatc gccgcgcgcc
      121 aggcatccag attgcgcggg tcgacgaatg tcgtctccac gccgaagcgc ggcagcgtgt
      181 acgcgaggag gttgtgcgag ccgccatata gcgcgcggct ggcgacgata tgcgagcccg
      241 cgcccatcag ggtaacgatg gcgaggtgca gcgccgcttg tccgctcgcg gcggcgatcg
      301 cgccgacgcc gttctcgagg gcggccatgc gctcctcgag caccgccacc gtcgggttcg
      361 agatgcgcga gtacacgtgg cccgcacgct ccatgttgaa cagcgccgca gcgtgatcgg
      421 agtcgtcgaa gcagaacgac gtcgatgcgt aaatcggcac tgcgcgcgcg ccggtcgcgg
      481 tgtcgggccg ctgccccgcg tgcaatacaa gcgtgtcgaa gccggggtat cggggaacgg
      541 tcatcggcgt cgtccgtcgg acatggattc gcgctaggtt accggagacg cgcaatgaac
      601 ctctgcttta tattgcttcg ccatggacat gcgcgctgat ctttacccgc cgctggagcc
      661 gtacgacagc ggcatgctgg cgctcgacga cgttcacacg atgttctggg aggcgtgcgg
      721 caatccgcac ggcatcccgg tgctgttcct gcacgggggg ccgggcggag gatgctcgcc
      781 cgagcatcgg cgcttcttcg accccgacgt gttccgcgtc gtgctgttcg atcagcgcgg
      841 cgccggcaat tccacgccgc tgggggacgt gaccaacaac accacgcaac acctcgttcg
      901 cgacatcgag ttgctgcgca gttacctcgg cttcggcaag gcgctggtct ttggcggctc
      961 gtggggcagt acgctcgcgc tcgcctacgg gcagacgcat ccggaccgtt gcctgggttt
     1021 cgtgctgcgc ggcgtgttcc tcggccgcgc ctcggagatc gagtggttcc tctacgggtt
     1081 gcggcacatc tttcccgagg cctggcgcgc gttcgccgag atgctgcccg aagaggagcg
     1141 tggcgacctg ctgcgcgggt acctgaagcg cctgttcgac cccgacccgc gcgtgcacgt
     1201 gccgttcgcg cgcgcatgga gcgagtacga gggactgtgc tcgacgctgc tgccgaaccc
     1261 ggatctggta cgccacttcg ccgacgaggc ggtcgggctg gcgcggctgg aagcgcatta
     1321 cttcgcgcat cagtgctttc ttgcccccaa ccagctgctc gccaatctgt accgcgtgcg
     1381 ccatgtgcca gtttcgatcg tccagggccg ctacgacatg gtgtgcccga tcgtcagcgc
     1441 cgacgacctc gcgcgggcat ggcccggggc acgctacgtc gtcgtcaacg acgctggcca
     1501 ctcggtctgg gagccggcaa tccgctcggc ggtgatgcgc gaagtggaac tgttcaagca
     1561 ggcgctcggg tgaggcggcc gcttgtgagg gggtcgggca ctgggc
//
LOCUS       DBNHBY010000802         3280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1011148, whole genome shotgun sequence.
ACCESSION   DBNHBY010000802 DBNHBY010000000
VERSION     DBNHBY010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1011148"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC16HB_18725"
     CDS             complement(<1..309)
                     /locus_tag="AC16HB_18725"
                     /inference="COORDINATES: protein motif:HMM:NF023395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5608882.1"
                     /translation="MAETVFAKLIRGELPCAKVYEDAHVFAFMDAGQVNPGHVIVASR
                     QPYETLLDADEATAAALFVAAHGIARAVQRAFQPAGFTILQANKPAGWQTVPHLHLHV
                     L"
     gene            complement(425..916)
                     /locus_tag="AC16HB_18730"
     CDS             complement(425..916)
                     /locus_tag="AC16HB_18730"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR5608883.1"
                     /translation="MSKNGKKGSASGIGLADKSRRTLVDVLNRLLADEFVLYVKTRNF
                     HWNVVGPNFSELHKFFEGQYDSLDEAIDEVAERARALDGMAAGSMTDYLKLTRLPEAS
                     SHLSAPEMLRALLADHEQVIRQLRDDIETAGDAGDKGTEDFLTGLMEVHEKTAWMLRS
                     YAR"
     gene            complement(1006..1950)
                     /locus_tag="AC16HB_18735"
     CDS             complement(1006..1950)
                     /locus_tag="AC16HB_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5608884.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELG
                     VLLFERRSNDVTLTPSGEQIVAQAQRVLDEASRIKEIAKQGMDPLAGPLRIGVIYTIG
                     PYLLPPLVRQLLRDAPQMPLLLSENFTIKLLEMLKNGEIDVAIMALPLPDSGVMIRPV
                     YDEPFVVVVPRDHAWTTRRSIASEDLKKETMLLLGTGHCFRDHVLGVCPELSRFSADA
                     EGIQKTFEGSSLETIRHMVASGLGITVLPVTSVPEKVRRDSLVAYLPFKAPAPDRRVV
                     LAWRKSFTRMPAIEVLRQAILKCPLHGVTKLDLPPTPG"
     gene            complement(1947..>3280)
                     /locus_tag="AC16HB_18740"
     CDS             complement(1947..>3280)
                     /locus_tag="AC16HB_18740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5608885.1"
                     /translation="ARGELVARLLAALPFKLTAAQQGVWHEVEADLARRAPMHRLVQG
                     DVGSGKTVIAALAAARAIEAGFQAALMAPTEILAEQHFRRIAEWLAPLGVRIAWLAGK
                     LKAGDKRAAHARVATGEAQLVVGTHALIQEGVQLARAGLAIIDEQHRFGVAQRLALRD
                     QGRAEATPHLLMLSATPIPRTLALSYLADLDVSVIDALPPGRTPVVTKLVATRRRAEV
                     LERVRTDVARGRQAYWVCPLVDEGEDEGANVDLTAATQMYEETRALLPELRFGLLHGQ
                     LPPAEKNEVMQRFARGAIDVLVATTVIEVGVDVPNATLMVIEHAQRFGLATLHQLRGR
                     FGRGTEQSFCVLLFDEPLTDAARERLKVVYETSDGFEIARRDLQLRGPGEFLGARQAG
                     VPLLRFADLEQDVALVEQARDVAQRLLEERPEVARAHARRWLAGRENYLDA"
BASE COUNT          494 a         1188 c         1065 g          533 t
ORIGIN      
        1 cagcacgtgc agatgcaggt gcggcaccgt ctgccagccg gccggcttgt tcgcctgcag
       61 gatcgtgaat ccggccggct gaaatgcgcg ctgcaccgct ctcgcgatcc cgtgcgcggc
      121 gacaaaaagt gccgccgcgg tcgcctcgtc cgcatcgagc agcgtctcgt acggctggcg
      181 gctggcgacg atcacgtgcc ccggattgac ctgccccgca tccatgaacg cgaacacgtg
      241 cgcgtcttcg tagaccttcg cgcagggcag ctcgccgcgg atcagcttcg cgaagacggt
      301 ctcggccatg gcgtcacctc aggccggcgc gcggcatgca cgcggcagat tgccgaccgc
      361 cacgcccgcg gcgcatgacc caagccgaac ttgggcaagc ggtcgcccga agacggcttg
      421 tgcgtcaccg ggcgtatgag cgcagcatcc aggccgtctt ctcatgcact tccatcaacc
      481 ctgtcaggaa atcctcggta cccttgtcgc cggcgtcgcc ggcggtctcg atatcgtcgc
      541 gcaactgacg gatcacctgc tcgtggtcgg ccagcagcgc gcgcagcatc tcgggcgcgg
      601 agagatggct cgacgcctcg ggcaggcgtg tcagcttcag gtagtcggtc atcgagcccg
      661 cggccatgcc gtcgagcgcg cgcgcgcgct ccgcgacctc gtcgatggcc tcgtcgagcg
      721 aatcgtactg cccctcgaag aacttgtgca gctccgagaa gttcggtccg accacgttcc
      781 agtgaaagtt gcgggtcttc acgtacagca cgaattcgtc cgccagcagg cggttcagca
      841 cgtcgaccaa cgtccggcgc gacttgtctg ccagcccgat gccgctcgcg cttcctttct
      901 tgccgttctt gctcattgcg tcctccttgc ctggaatgaa tccaacctta gcgcagcgcg
      961 gcgggccgtg cattgagttg tttcaatcgg cgtcatagcc gagggtcaac ccggggtcgg
     1021 cggcaggtcg agcttggtca ccccgtgcag tgggcacttg aggatcgcct gccgcagtac
     1081 ctcgatcgcg ggcatccgcg tgaagctctt gcgccacgcc agcaccacac gccggtcggg
     1141 cgccggtgcc ttgaacggca ggtacgcgac cagcgaatcg cggcgcacct tctccggcac
     1201 cgacgtgacc ggcagcacgg tgatgccgag ccccgaggcg accatgtggc ggatcgtctc
     1261 gagcgacgaa ccttcgaacg tcttctggat gccctccgca tcggccgaga aacgcgacag
     1321 ctccgggcac accccgagca cgtgatcgcg gaaacaatgc ccggtgccga gcagcagcat
     1381 cgtctccttc ttcaggtcct ccgacgcgat gctcctgcgc gtcgtccacg catgatcgcg
     1441 tggcaccacg accacgaacg gctcgtcgta gacgggccgg atcatcacgc ccgagtccgg
     1501 cagtggcagc gccatgatcg ccacgtcgat ctcgccgttc ttcagcatct ccaggagctt
     1561 gatggtgaag ttctcgctca gcagcagcgg catctgcggc gcgtcgcgca gcagctggcg
     1621 caccagtggc ggcagcaggt aggggccgat cgtgtagatc acgccgatgc gcagcggacc
     1681 cgcgagcgga tccatgccct gcttggcgat ctccttgatg cgtgacgcct cgtcgagcac
     1741 gcgctgcgcc tgcgccacga tctgctcgcc gctcggcgtc agcgtcacgt cgttgctccg
     1801 gcgttcgaac agcagcacac cgagctcgtc ttcgagcttc ttgatcgcca ccgacagcgt
     1861 cggctgcgac acgaagcacg cctcggcggc gcggccgaag tggcgctcgc gcgccacggc
     1921 gacgatgtag cggagttcgg tcagggtcat gcgtcgagat aattctcacg tcctgcgagc
     1981 cagcgtcgcg catgcgcgcg cgcgacctcc ggtcgttctt cgagcagtcg ttgcgccacg
     2041 tcgcgcgcct gctcgaccag cgcgacgtcc tgctccaggt cggcgaagcg tagcagcggc
     2101 acgcccgctt gccgcgcgcc gaggaactcc cccgggcctc gcaactgcag atcgcgccgc
     2161 gcaatctcga agccgtcgga cgtttcgtag acgaccttca gccgctcgcg cgctgcgtcc
     2221 gtcaatggct cgtcgaacag cagcacgcag aaactctgct cggtgccccg gccgaagcgg
     2281 ccgcgcagct ggtgcagcgt ggcgagcccg aagcgctgcg cgtgctcgat caccatcaac
     2341 gtcgcgttcg gtacgtcgac gcccacctcg atcacggtcg tcgccaccag cacgtcgatg
     2401 gcgccgcgcg cgaagcgctg catcacctcg ttcttttccg ccggcggaag ctggccgtgc
     2461 agcagcccga agcgtagctc cggcagcagc gcgcgcgtct cctcgtacat ctgggtggcc
     2521 gcggtcagat cgacgttggc gccctcgtcc tcgccttcat cgaccagcgg gcagacccag
     2581 tacgcctggc ggccgcgcgc gacatcggtg cgcacgcgtt cgagcacctc ggcgcggcgc
     2641 cgcgtggcga cgagcttcgt caccaccggc gtgcgtccgg gcggcagcgc atcgatcacc
     2701 gaaacgtcga ggtcggcgag atagctcagc gccagcgtgc gcgggatcgg cgtggcgctg
     2761 agcatcagca ggtgcggcgt cgcctcggcc cgaccctggt cgcgcagcgc cagccgctgc
     2821 gccacgccga accggtgctg ctcgtcgatg atcgccagcc cggctcgtgc gagctgcacg
     2881 ccttcctgga tcagtgcgtg tgtgccgacc acgagttgcg cctcgccggt ggccacacgc
     2941 gcgtgcgcgg cgcgcttgtc gcccgccttc agcttgccgg ccagccaagc aatgcgcacg
     3001 ccgagcggcg ccagccactc ggcgatgcgg cggaagtgct gctccgcgag gatctcggtc
     3061 ggcgccatca gcgcggcctg gaagcccgcc tcgatcgcgc gcgccgcggc cagtgccgcg
     3121 atcacggtct tgccgctgcc gacatcgcct tgtacgagcc ggtgcatcgg cgccctgcgc
     3181 gcgagatcgg cctcgacctc atgccacacg ccctgctgcg ccgcggtcag cttgaacggc
     3241 agcgcggcca gcagccgcgc caccagttcg ccgcgcgcgg
//
LOCUS       DBNHBY010000803         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_740056, whole genome shotgun sequence.
ACCESSION   DBNHBY010000803 DBNHBY010000000
VERSION     DBNHBY010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_740056"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1310
                     /gene="mutL"
                     /locus_tag="AC16HB_18745"
                     /pseudo
     CDS             200..1310
                     /gene="mutL"
                     /locus_tag="AC16HB_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402018.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
     gene            1440..>1683
                     /gene="mutL"
                     /locus_tag="AC16HB_18750"
     CDS             1440..>1683
                     /gene="mutL"
                     /locus_tag="AC16HB_18750"
                     /inference="COORDINATES: protein motif:HMM:NF020260.7"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR5608886.1"
                     /translation="MQDAAAATAPASSAERPLGSQDAERPLGVAIAQLAGIYILAQNS
                     AGLVIVDMHAAHERIVYEKLKRQLDSHSIPQQRLLIP"
BASE COUNT          269 a          591 c          584 g          239 t
ORIGIN      
        1 agacgcgccc ggtctcggag aagaagatcg ccgccagcac cacgtacacc agcagcacgt
       61 tattgtgcac gatggcaagc aggaacttgt ccatcgcgtt gaacaggtcg gaccagttga
      121 tgccttccat cgcgatgcgc tgggcgggaa caaaaagatg gcggagtgcc cgatcgggca
      181 ccccgccgag atgctgccga tgatacgcga gcgatccggg caacgatctc gcgatcgacc
      241 gagggaattc actgatcgcg cggcccgcgg ttcctataat cctcgcgatg tcgccctctt
      301 ccatcctcgc ggcgagcgcg cgccgctcga tccgacccct gcccgacctg ctggtgagcc
      361 agatcgccgc cggcgaggtg atcgagcggc cggcctcgat cgtcaaggaa ctggtcgaga
      421 acgcgctcga cgccggtgcc acgcgcatcg acgtgcggct cgacggcggc ggcatccgcc
      481 gcatcgtggt gagcgacgac ggctgcggca tcccgcgcga ggaactgccg ctggcgctgg
      541 cgcgccatgc gacgagcaag atcgcatcgc tgcgcgacct ggaagcggtg gcgtcgctcg
      601 gttttcgcgg cgaggcattg gcgtcgatcg cgtcggtcgc cgcggtgacg atttcctcgc
      661 gcaccccgga cgcgcccggc gcgttccgca tcgacgccga ggcgggcgcg atcgagcccg
      721 cggccggaac gccgggcacg cgtgtcgagg tcgcggatct gttcttcaaa acgccggcgc
      781 ggcgcaagtt cctgcgcgcg gagaacacgg aactcgggca ctgcgtggtg caggtcgagc
      841 gcatcgccgc ggcgcatccg caggtcgagt tcaacgtcgc gcacgaaggt cgcacggtgc
      901 tcgcgctgcc gccatcgagc gtcgccgagc gcacgcgccg gctgatgccg gacgcgttcg
      961 acgctgcacg gcgcgaagtg cgcgccgagg cgcctgacat ccacgtccgc ggctgggtcg
     1021 gcgcgcccac cgccgcgcgt gcgcgcgccg atgcgcagct cttctacgtc aacggccgct
     1081 acgtgcgcga caaggtgctc acccatgcgg tgcgcgccgc gtatgcggac gtgctgcatg
     1141 ggcaggcgca gccgatgtac gtgctctatc tcgacatcga tccgcagcgc gtcgacgtca
     1201 acgtgcaccc gaccaagatc gaggtgcgct tccgcgactc gcaggcggtg caccagttcg
     1261 tgctgcatgc agtgcaggcg gcgctcgcgg agtccgcgcg cgccgcgcct gcgcatgcgc
     1321 cggccgcgct cacggaactc tcggcaatcc gcgacgccgc gcagcgcgcc gcgcgtgaca
     1381 gcgcgcagca gcggacgctc ggcatcgcgc agccggtggc cgagtatttc gcgatggcaa
     1441 tgcaggatgc ggccgcggcg accgcaccgg catcgagcgc cgagcgaccg ctcggaagtc
     1501 aggacgccga gcgaccgctc ggcgtggcca tcgcccagct cgccggcatc tatattctgg
     1561 cgcagaacag cgcggggctc gtgatcgtcg acatgcacgc cgcgcacgag cgcatcgtgt
     1621 acgagaagct gaagcgccag ctcgactcgc attcgatacc gcagcagcgg ctgctgatcc
     1681 cgc
//
LOCUS       DBNHBY010000804         3596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1484349, whole genome shotgun sequence.
ACCESSION   DBNHBY010000804 DBNHBY010000000
VERSION     DBNHBY010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1484349"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..473)
                     /gene="dtd"
                     /locus_tag="AC16HB_18755"
     CDS             complement(24..473)
                     /gene="dtd"
                     /locus_tag="AC16HB_18755"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5608887.1"
                     /translation="MIALIQRVSRARVCVDGAVTGEIGPGLLVFVCAERADTEREADA
                     LLAKLLAYRVFADADGKMNRSVRDVDGELLIVSQFTLAADTNSGTRPSFTPAAAPETG
                     RRLYERFVAQARLAHRQVATGAFGAMMQVELVNDGPVTFWLQVRPRT"
     gene            complement(499..1731)
                     /gene="tyrS"
                     /locus_tag="AC16HB_18760"
     CDS             complement(499..1731)
                     /gene="tyrS"
                     /locus_tag="AC16HB_18760"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5608888.1"
                     /translation="MSGPSHPITPQIERALALIRRGCDELLIESEFAQKLARSAATGK
                     PLRCKLGLDPTAPDIHLGHTVVLNKLRQLQDLGHTVIFLIGDFTSMIGDPSGRNTLRP
                     PLSREEIETNAKTYFDQAALVLDRDKTEIRYNSEWSEPLGAAGMIKLAARYTLARLLE
                     RDDFAKRMAAGQPIAMHELLYPLMQGYDSVALHADLELGGTDQKFNLLVGRELQRHYG
                     QEPQCILTMPLLEGLDGVEKMSKSKGNYVGITEPPFEMFGKVMSISDEQMWRWYDLLS
                     MRGNEEIAALRRQVGEGRNPRDIKVLLAKEIVARFHTARAADQAEFEFMARFRAGAVP
                     NEMPAFTLATEGKGAAVAALLKQAALAPSTSEAIRNVEQGGVKIDGVKVADRALLVRP
                     GTYVVQVGKRKWARVTVT"
     gene            1791..2900
                     /locus_tag="AC16HB_18765"
     CDS             1791..2900
                     /locus_tag="AC16HB_18765"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR5608889.1"
                     /translation="MADSELFIGLMSGTSLDGVDGVLADFAAPRPRLLAHAQHGFAPE
                     LRAAFEHLLHPGTDELHRAEVAAQHLARAYASVVQELLLHAGVDPEQVRAIGAHGQTV
                     RHRPDAGYTVQLNSPAVLAELTGIDVVADFRSRDIAAGGQGAPLVPAFHAALFATNAP
                     RAVINLGGISNVTGIPPADGDGSVTGFDCGPGNTLIDAWARRHLGEPFDRDGRWAASG
                     HTNAALLESMWAEPYFSLAPPKSTGRELFNIAWLDAHLDGRNLAPRDVQATLTHLTAR
                     AAGQAIARYLPEARDVIACGGGTRNGALMKMLADECAPRPVLSSEQLGVDPQHVEALA
                     FAWLARAHLARHAGSLPAVTGARGARVLGALYPAG"
     gene            complement(2956..3306)
                     /gene="erpA"
                     /locus_tag="AC16HB_18770"
     CDS             complement(2956..3306)
                     /gene="erpA"
                     /locus_tag="AC16HB_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR5608890.1"
                     /translation="MNAAVEMPAPLVFSDSAAAKVKQLIEEEGNPDLKLRVFVQGGGC
                     SGFQYGFTFDEVVNDDDATMEKNGVMLLIDSMSYQYLVGAEIDYKEDLEGAQFVIRNP
                     NAQTTCGCGSSFSV"
     gene            complement(3375..>3596)
                     /locus_tag="AC16HB_18775"
     CDS             complement(3375..>3596)
                     /locus_tag="AC16HB_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5608891.1"
                     /translation="VYASELVELQPKARIVGDVHYKALEMHHGAVVEGMLVHQSVQEA
                     PRTIGLKLAVGAPKLEVMSGEPKAESGRG"
BASE COUNT          559 a         1251 c         1192 g          594 t
ORIGIN      
        1 tgagtctcat cgtgggcgcg ctgctacgtg cgtggcctga cctgcagcca gaacgtgacc
       61 ggaccgtcgt tgaccagttc gacctgcatc atcgcaccga aagcgccggt ggcgacctgg
      121 cgatgcgcga gccgcgcctg cgcgacgaag cgctcgtaca gacgccggcc ggtctcgggc
      181 gcggctgccg gcgtgaagct cggccgcgtg ccggaattcg tgtcagcggc gagcgtgaac
      241 tgcgaaacga tcagcagttc accgtcgacg tcgcgcacgc tgcggttcat cttgccgtcc
      301 gcgtccgcaa acacccgata cgcgagcagc tttgcgagca gcgcgtccgc ctcgcgctcg
      361 gtgtcggcgc gctcggcgca cacgaacacc agcagccccg ggccgatctc gccggtcacc
      421 gcgccgtcga cgcacacgcg cgcccgcgac acccgctgga tcagcgcgat cattgcgatc
      481 ccgcgcgatt ccggtcgctc aggtcaccgt cacgcgcgcc cacttgcgct tgccgacctg
      541 caccacgtac gtgccgggcc gcaccagcag cgcgcgatcg gcaaccttca cgccgtcgat
      601 cttcacaccg ccctgctcga cgttgcggat cgcctcgctg gtcgacggcg cgagcgcggc
      661 ctgcttcagc agcgccgcga ctgcggcgcc cttgccctcg gtcgccagtg tgaacgcggg
      721 catctcgttc ggaacggcgc cggccctgaa gcgcgccatg aactcgaact cggcctgatc
      781 ggccgcgcgc gccgtatgaa agcgcgcgac gatttccttc gccagcagca ccttgatgtc
      841 gcgcggattg cgaccctcgc cgacctggcg gcgcagcgcc gcgatttctt cgttgccgcg
      901 catcgacagc aggtcgtacc agcgccacat ctgctcgtcg gagatcgaca tcaccttgcc
      961 gaacatctcg aacggcggct ccgtgatgcc gacgtagttg cccttgctct tcgacatctt
     1021 ctcgacgccg tcgagccctt cgagcagggg catggtcagg atgcattgcg gctcctggcc
     1081 atagtggcgc tgcagttcgc ggcctaccag caggttgaac ttctggtcgg tgccgccgag
     1141 ttccagatcc gcgtgcagcg ccaccgagtc gtaaccctgc atcagcgggt acagcaattc
     1201 gtgcatcgcg atcggctggc cggcagccat gcgcttggcg aagtcgtcgc gctcgagcag
     1261 ccgcgccagc gtgtacctgg cggcgagctt gatcatgccg gcggcgccga gcggctccga
     1321 ccactcggag ttgtagcgga tctcggtctt gtcgcgatcg agcacgagcg ccgcctgatc
     1381 gaaataggtc ttcgcgttgg tctcgatctc ctcgcgcgac agcggcggcc gcagtgtgtt
     1441 gcggccagac gggtcgccga tcatcgaagt gaagtcgccg atcaggaaga tgaccgtgtg
     1501 gccaaggtcc tgcagctggc gcagcttgtt cagcacgacc gtgtgcccga ggtggatgtc
     1561 cggcgcggtc ggatcgagcc cgagcttgca gcgcagcggc tttcccgtcg cggccgagcg
     1621 cgccagcttc tgcgcgaact ccgattcgat cagcagttcg tcgcagccgc gccgaatcag
     1681 cgcgagcgca cgctcgatct gcggcgtgat gggatgggac ggacccgaca tgatttgcgg
     1741 cacgtgcgcc ggatggcgcg atcgaaggag gcggagtcta attgatgccc atggccgact
     1801 cggaactgtt catcggtctg atgtccggga ccagcctcga cggcgtcgac ggggtgctgg
     1861 ccgacttcgc cgcgccgcgt ccgcgcctgc tggcccacgc ccagcatgga ttcgcgccgg
     1921 aactgcgcgc agcgttcgag catctgctgc atccgggcac ggacgaactg caccgtgccg
     1981 aagtcgccgc gcagcatctg gcgcgcgcgt acgcgtcggt ggtgcaggag ctgctgctgc
     2041 acgcgggcgt ggacccggag caggtgcgcg cgatcggcgc gcacggacag acggtgcgcc
     2101 accggcccga cgccggctac acggtgcagt tgaacagccc ggccgtgctc gccgagctga
     2161 ccggcatcga cgtcgtcgcg gatttccgta gccgcgacat cgccgccggc ggccagggcg
     2221 ccccgctcgt gccggcgttc cacgccgccc tgttcgcgac caacgcgccg cgcgccgtga
     2281 tcaatctcgg cggcatcagc aacgtgaccg gcattccgcc cgcggatggc gatggcagcg
     2341 tgaccggatt cgactgcggc ccgggcaaca ccttgatcga cgcgtgggcg cgccgccacc
     2401 tgggcgaacc atttgaccgc gacggtcgct gggccgcgtc gggacatacg aatgccgcgc
     2461 tgctcgaatc gatgtgggcg gagccgtact tctcgctggc gccgccgaag agcaccggcc
     2521 gcgagctctt caacatcgca tggctcgacg cacacctgga cggccgcaac ctcgcgccga
     2581 gggatgtgca ggcgaccctg acgcacctca ccgcccgggc cgcgggacag gcgatcgcac
     2641 gctatcttcc ggaagcacgc gacgtgatcg cctgcggcgg cggcacgcgc aatggcgctc
     2701 tgatgaagat gctggccgac gaatgcgcgc cgcgaccggt actgtccagc gaacaactcg
     2761 gggtcgaccc gcagcacgtc gaggcgctgg cattcgcatg gctggcgcgc gcgcatctgg
     2821 cgcggcacgc cggcagcctg cccgccgtca ccggagcacg cggcgcgcgc gtactcggtg
     2881 cactgtaccc ggccgggtga gaaacaaaaa cggggcgcaa cgcgccccgt tcctggcgat
     2941 gccagtgccc gtgcgctaaa ccgagaacga cgatccgcag ccgcaggtcg tctgcgcgtt
     3001 cgggttgcgg atcacgaact gggcgccctc gaggtcctct ttgtagtcga tctcggcgcc
     3061 gaccaggtac tggtagctca tcgagtcgat cagcagcatc acgccgttct tttccatcgt
     3121 cgcgtcgtcg tcattgacga cctcgtcgaa cgtgaagccg tactggaagc cggaacaacc
     3181 gccgccctgc acgaacacgc gcagcttcag gtcggggttg ccttcctcct cgatcagttg
     3241 cttgaccttg gcggccgcgg agtccgaaaa gaccagcggg gcgggcatct caacggctgc
     3301 gttcatggcg tactcctgga aggcgttgcc gcgattatag ggacggaaag tcgcccgggt
     3361 tgacgggcgt cagatcaccc tctgccggac tcggcctttg gctctcccga catgacttcc
     3421 agtttggggg cgccgaccgc aagcttcaag ccgatcgtcc gcggggcttc ctgcacggac
     3481 tggtgcacca gcatgccctc gacgacggcg ccgtggtgca tttcgagcgc cttgtagtgc
     3541 acgtcgccga cgatgcgcgc tttcggctgc agttccacca gttcggaggc atagac
//
LOCUS       DBNHBY010000805         2789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_874532, whole genome shotgun sequence.
ACCESSION   DBNHBY010000805 DBNHBY010000000
VERSION     DBNHBY010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_874532"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..584)
                     /locus_tag="AC16HB_18780"
     CDS             complement(12..584)
                     /locus_tag="AC16HB_18780"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR5608892.1"
                     /translation="MQVNPKQGPDVAADDCREALSCLIDGELNHAECARLLDRICADP
                     RVREQWALLHTAGDALRSSDVAALHSSAFVGRVAAALAREPTILAPRALSRRRFQRVL
                     IPGAAVAAAAALLVVVAMPQLRGAGEAPQIAATTPAAAPASTPQPIEVARVPELERYL
                     RAHREMAGSLVMPDATPYLRTSNNLPGETR"
     gene            complement(592..1191)
                     /gene="rpoE"
                     /locus_tag="AC16HB_18785"
     CDS             complement(592..1191)
                     /gene="rpoE"
                     /locus_tag="AC16HB_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR5608893.1"
                     /translation="MSERDVDQQLVERVQRGDKRAFELLVAKYQRKIFRLLSRLIRDQ
                     SEIEDVAQEAFIKAYRALPNFRGESAFYTWLYRIAINTAKNFLVSQGRRPPTTTQNEV
                     EEAENFDDATLLRDVDTPDSMLMSKQVGDAVNRAIGRLPDDLRTAIVLRELEGMSYEE
                     IAAAMNCPIGTVRSRIFRAREAIAEELRPLLDTSKDKRW"
     gene            complement(1296..1652)
                     /locus_tag="AC16HB_18790"
     CDS             complement(1296..1652)
                     /locus_tag="AC16HB_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608894.1"
                     /translation="MVLTVSAFVIACLSTLSAPVPGRILAAAASFIALAAGWLAWRRA
                     AAPLQLRVTRDAEILARAGDTAEEQSLQVRFVAPWLIVLAGGRMFVPVWPDCLGANEY
                     RRLAVACRWRRPVATA"
     gene            complement(1717..>2789)
                     /gene="fabF"
                     /locus_tag="AC16HB_18795"
     CDS             complement(1717..>2789)
                     /gene="fabF"
                     /locus_tag="AC16HB_18795"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5608895.1"
                     /translation="KEFDITAYISAKEARRMDTFIHYGMAASIQAIRDSGLEITDANR
                     EQIGCMIGSGIGGLPLIEENYQEFAARGARRISPFLIPGAIINMISGNLSILYGLKGP
                     NLAIVTACTTGLHCIGESGRLIEYGDADVMVAGGAEATVCPLGVGGFASMQALSVRND
                     DPATASRPFDRDRDGFVLGEGAGVMVLEEYEHARKRGARIYCEVLGMGMSADAYHMTA
                     PDVDGPRRAMLGALKRAGVNADEVDYLNAHGTSTPLGDTNETNAIKLALGDAARKVVV
                     NSTKSMTGHLLGGAGGLESVVTALAIHHQVSPPTINIFNRDPECDLDYAANTAREMKI
                     GVALKNSFGFGGTNGTLVFKRV"
BASE COUNT          441 a          973 c          924 g          451 t
ORIGIN      
        1 gccgcctgcc atcaacgcgt ctccccaggc aggttgttgg acgtgcgcag atacggcgtg
       61 gcgtcgggca tcaccagact gcccgccatc tcacgatgcg cgcgcaggta gcgctcgagt
      121 tcgggaacgc gcgcgacttc gatcggctgc ggcgtgctgg ccggcgcggc ggcgggtgtg
      181 gtcgccgcaa tctgtggagc ctcgccagcc ccgcgcaact gtggcatggc gacaacgacc
      241 agcagcgcgg ccgccgccgc caccgcggcg cccgggatca gcactcgctg aaagcggcgc
      301 cgcgaaagcg cacgcggcgc caggatcgtg ggctcgcgcg ccagcgccgc cgccacgcgg
      361 ccgacgaacg cgctcgaatg cagcgctgcg acatcgctcg aacgcagtgc gtcgcccgcg
      421 gtgtgcagca aggcccactg ctcgcgcacg cgcgggtcgg cgcagatgcg atcgagcaac
      481 cgcgcgcatt ccgcgtgatt caactcgccg tcgatcaggc acgacagtgc ttcgcggcaa
      541 tcgtcggccg cgacgtccgg gccttgcttc ggattcacct gcatgacgac ctcaccaacg
      601 cttgtccttc gacgtgtcca gcagcggccg caactcctcc gcaatcgcct cgcgcgcgcg
      661 aaagatgcgg gagcgtaccg taccaatcgg gcagttcatt gccgccgcga tctcttcgta
      721 actcattccc tccaattcgc gcaggacgat cgctgtgcgc aggtcgtccg gcaggcgccc
      781 gatcgcacga ttgaccgcgt cgcccacctg cttggacatc agcatggagt ccggcgtgtc
      841 gacgtcgcgc agcagcgtgg cgtcgtcgaa gttctccgcc tcctccacct cgttctgggt
      901 ggtcgtcggc gggcgacggc cttgggagac gaggaagttc ttggcggtgt tgatcgcgat
      961 ccgatacagc catgtgtaaa acgcgctttc gccgcgaaaa ttgggcagtg cccggtacgc
     1021 cttgatgaat gcttcctgcg ctacgtcctc gatctcggac tggtcccgga tcagccgcga
     1081 gagcaggcgg aagatcttgc gctggtactt cgccaccagc agctcgaacg cccgcttgtc
     1141 gccgcgctgg acccgctcga cgagctgctg gtcgacgtcg cgctcgctca tcgatcagct
     1201 ccgcgcgccg cagagcggtg tgcgtccgaa tctgaccggc cggccacgaa taagtttcgc
     1261 ccggccgtgc ggccaggcgg atgctttgac gctggtcacg ccgtcgccac gggccggcgc
     1321 caacggcagg ccaccgcaag ccgccggtac tcgttcgcgc cgaggcaatc cggccagacc
     1381 ggaacgaaca tgcgtccgcc ggcgagcacg atcagccatg gggcgacgaa ccggacctgc
     1441 aaggactgct cctcggccgt gtcgcccgcg cgcgcgagga tttccgcgtc ccgcgtcacc
     1501 cgcagctgca gcggcgcggc ggcacgacgc catgccagcc agccggcagc cagcgcgatg
     1561 aaggacgcgg cggcggccag gatgcggccc ggcacgggcg cactgagcgt ggacaggcag
     1621 gcgatgacga aggcgctgac tgtcagcacc atcccgccgg ccatgagccg gcgagccaac
     1681 tccgaccgcc gtagcgtggt ccacgggaaa gcgatgtcag acacgcttga agaccagcgt
     1741 gccgttcgtg ccgccaaagc cgaaggagtt cttgagcgcg acgccgatct tcatctcgcg
     1801 cgcggtgttg gccgcatagt cgagatcgca ctcggggtcc cggttgaaga tgttgatcgt
     1861 cggtggcgag acctggtgat gaattgccag cgcggtcacc accgactcga gtccgcccgc
     1921 gccgccgagc aggtggccgg tcattgactt cgtcgagttg acgaccacct tgcgcgccgc
     1981 gtcgccgagc gcgagcttga tcgcattggt ctcgttggtg tcgccgagtg gcgtcgatgt
     2041 tccgtgcgcg ttcagatagt caacctcgtc ggcgttgacg ccggcgcgct tcaacgcgcc
     2101 gagcatcgcg cgccgcgggc catcgacgtc gggcgcggtc atgtggtagg cgtcggcgct
     2161 catccccatg ccgagcacct cgcagtagat gcgcgcgccg cgcttgcgcg cgtgctcgta
     2221 ctcctcgagc accatgacgc ccgcgccctc acccagtacg aagccgtcgc ggtccctgtc
     2281 gaacgggcgg ctcgcggtcg cgggatcgtc gttgcgcacg gacagcgcct gcatcgacgc
     2341 gaagcccccg acaccgagcg ggcacacggt tgcctccgcc ccgcccgcca ccatgacgtc
     2401 ggcatcaccg tactcgatca gccggcctga ttcgccgatg caatgcagcc cggtcgtaca
     2461 ggcggtgacg atcgccaggt tcggaccctt gagcccgtac aggatcgaca ggttgcccga
     2521 gatcatgttg atgatcgcgc cgggaatcag aaacggcgag atgcggcgcg cgccgcgggc
     2581 cgcgaattcc tggtagttct cctcgatcag cggcagcccg ccgatgccgg agccgatcat
     2641 gcagccgatt tgctcgcggt tcgcatccgt gatttccagg cccgagtccc ggatcgcctg
     2701 gatcgacgcg gccatcccgt agtggatgaa ggtgtccatc cgccgcgctt ccttggcgga
     2761 gatgtacgcg gtgatgtcga actccttga
//
LOCUS       DBNHBY010000806         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_401777, whole genome shotgun sequence.
ACCESSION   DBNHBY010000806 DBNHBY010000000
VERSION     DBNHBY010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_401777"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="AC16HB_18800"
     CDS             complement(<1..1642)
                     /locus_tag="AC16HB_18800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     sulfotransferase family protein"
                     /protein_id="HIR5608896.1"
                     /translation="MRFAPSDAGALNLTGLALHAQARNDEAAHVFVRLLHEDRDNRAH
                     WVNYGTILRAIGRYEEAQRAYGRAAALGEDSPDLSYNVGLLHLDRGDAESARLELQRA
                     HAARPDDPDIACEYAAACCETLHTDEGLAALRRWRSFQPLDTERTARVATLLLSLGDG
                     AGADEALAHALSDAAPSAAARVQLALALERVNRLEAAQQQLDSIQAELSGPLGRDLLL
                     AKARLAQRAGRHDEAVRLFGELARRETIERTHAHLFPLARSLDALGRYDEAYAALCEA
                     HASQARWIRHTQPEIVRRKSDTLRITRRGVDPVDVARWDHAGAPPAERSPIFIVAFPR
                     SGTTLLEQMLDAHPALQSMDEQPFLQQAIVGLERSGASYPERMADLTRVELDAARAEY
                     WRLVATRVELQPGQRLVDKNPLNLLRLPAIARLFPHASILVALRHPADVLLSGWMQHF
                     RPEFAWHCRDLSTLALAHQRAFDYWYAQSAMLQPRALEVRYERLVAGIAAEARRIVDF
                     LQLPWDASVLAPADHALRKGLIGTPSYAQVIRPVHSESV"
BASE COUNT          245 a          579 c          603 g          240 t
ORIGIN      
        1 cgacggattc cgaatgcacc ggacgaatca cctgcgcgta gctcggcgtg ccgatcagtc
       61 ccttgcgcaa ggcatggtcg gcgggcgcga ggaccgacgc atcccacggc aactgcagga
      121 agtcgacgat gcggcgcgct tcggcggcga tcccggcaac gaggcgctcg taacgcacct
      181 ccagcgcacg gggctgcagc atggcgctct gcgcgtacca gtagtcgaac gcgcgctggt
      241 gggccagcgc cagcgtcgac aggtcgcgac agtgccaggc gaactcgggg cggaagtgct
      301 gcatccagcc actcagcagc acgtcggccg gatggcgcag cgcgaccagg atcgaggcat
      361 gcggaaagag gcgcgcgatc gccggcaggc gcagcaggtt cagcggattc ttgtcgacca
      421 gccgctgtcc cggctgcagt tcgacacgag tcgcgaccag gcgccagtac tcggcccgcg
      481 cggcgtcgag ttcgacgcgc gtcaggtccg ccatgcgctc ggggtagctg gcgccggacc
      541 gctcgagccc gacgattgcc tgctgcagga acggctgctc gtccattgac tgcagcgcgg
      601 gatgcgcgtc cagcatctgc tcgagcagcg tcgtgccgga ccgcgggaac gcgacgatga
      661 agatgggact gcgttctgcc ggcggcgcgc ctgcgtggtc ccagcgcgcc acatcgaccg
      721 gatcgacccc gcggcgcgtg atccgcaacg tgtcgctctt gcgtcgcacg atctccggct
      781 gggtgtggcg tatccagcgc gcctgcgaag cgtgcgcctc gcacagcgcg gcgtaggctt
      841 cgtcgtagcg cccgagcgca tccagcgatc gcgccagcgg gaaaaggtgt gcgtgagtcc
      901 gctcgatcgt ctcgcggcgc gcgagttcgc cgaacaggcg gacggcttcg tcatgccgcc
      961 ccgcgcgctg cgccaggcgg gccttggcga gcaggaggtc gcggccgagt ggcccggaca
     1021 attccgcctg aatcgaatcc agctgctgct gtgccgcttc gagccggttg acgcgctcca
     1081 gcgcgagcgc gagctggacg cgtgccgcgg cgctcggcgc cgcgtcgctc agagcgtgcg
     1141 ccagcgcttc gtccgccccc gcgccatcgc cgaggctgag cagcagcgtc gccacgcggg
     1201 ccgtgcgctc cgtgtcgagc ggctggaagc tgcgccagcg gcgcagcgca gccagccctt
     1261 cgtcggtgtg cagcgtctcg cagcaggctg cagcgtactc gcaggcaatg tcgggatcgt
     1321 cgggacgcgc ggcgtgcgca cgctgcagtt ccaggcgcgc cgactccgcg tcgcctcgat
     1381 ccaggtgcag cagcccgacg ttgtaggaga ggtctggtga atcctcgccg agcgcagcag
     1441 cacgaccgta cgcgcgctgg gcttcctcgt agcgcccgat ggcgcgcagg atcgtcccgt
     1501 agttgaccca gtgcgcgcgg ttgtcacggt cctcgtgcag cagccggaca aagacgtgcg
     1561 cagcctcgtc gttgcgggcc tgcgcgtgca gcgccagtcc ggtcagattc agcgccccgg
     1621 cgtcggacgg cgcaaagcgc aaggcggctc gcccgcagcg ttcggcg
//
LOCUS       DBNHBY010000807         4507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_63662, whole genome shotgun sequence.
ACCESSION   DBNHBY010000807 DBNHBY010000000
VERSION     DBNHBY010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_63662"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="cysE"
                     /locus_tag="AC16HB_18805"
     CDS             complement(<1..665)
                     /gene="cysE"
                     /locus_tag="AC16HB_18805"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR5608897.1"
                     /translation="MFSRLREDIACILERDPAARSTWEVLTCYPGLHALVLHRLAHAC
                     WTRGLRWLGRFISQASRWFTGIEIHPGAKVGRRVFIDHGMGIVIGETAEIGDESTLYQ
                     GVTLGGTSLAKGAKRHPTLGRGVIVGAGAKILGGFTVGDGARVGSNAVVIKEVPPGAT
                     AVGNPARILHKEVEAAREQAANRMGFSAYGLSQDGDDPLTTALHGLIDHVAAQQREIE
                     RLVA"
     gene            complement(698..1465)
                     /locus_tag="AC16HB_18810"
     CDS             complement(698..1465)
                     /locus_tag="AC16HB_18810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR5608898.1"
                     /translation="MPLSIADRIRFVLVAPSHPGNIGAAARALKTMGWTRLAVVAPKV
                     AGFREHEAALAFASHAHDVLRAATVHADLADALSGVRLAFAMTGYAREFGPRLIDLRT
                     AVGEARVEALDGDIAFVFGTERDGLRNEDVERCYACVAIPTDLEYGSLNLAQAVQVVA
                     YECRLALADAGAVASRFAADPPATVDAVEALHAHLEAALVALGYLDPAQPKRLMARLR
                     RLLTRARPTASEVDILRGIAAAIIARKSERAGRKSAR"
     gene            1556..2377
                     /locus_tag="AC16HB_18815"
     CDS             1556..2377
                     /locus_tag="AC16HB_18815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010808970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5608899.1"
                     /translation="MHPMLNTAVKAARKAGAIINRASLDLDLVRVSAKGRSDFVTEVD
                     HAAEAAIIDTLKTAYPQHAILAEESGATKSDTGADYTWIIDPLDGTTNFIHGFPQYAV
                     SIALQHGGQITQAVVYDPTRNELFTATRGRGAFLNDRRLRVSRRDKLREALVGTGFPF
                     RQLDHLDEYVRMFKRITEETAGIRRPGAAALDLAYVAAGRLDGFWEFGLSPWDMAAGS
                     LLIIEAGGMVGDFLGEKRYLETGRIVCGAPKVYEQLLAMVRDVHLQDARARGPEA"
     gene            2374..2874
                     /locus_tag="AC16HB_18820"
     CDS             2374..2874
                     /locus_tag="AC16HB_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR5608900.1"
                     /translation="MNQFVETYLGTRRRVCAAVFIVIAVLLSTSLYLQHVVGLEPCPL
                     CILQRYAFVAVALFALIAALAPDLPSRMAHAASGLFALVGGGTAAWHVWLQVNPPKFA
                     SCGPGLEYMLSELPLARALPRIFQGSGDCTEVQWTFLGLSIAGWSLVWFTILFVALVA
                     ASRRRR"
     gene            2927..>4507
                     /gene="mutS"
                     /locus_tag="AC16HB_18825"
     CDS             2927..>4507
                     /gene="mutS"
                     /locus_tag="AC16HB_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR5608901.1"
                     /translation="MMQQYLRLKAQHPRALLFYRMGDFYELFYEDAERGAKLLNITLT
                     KRGTSAGAPIPMAGVPVVSVEQYLARLVKLGESVAICEQIGDPATSKGPVERKVVRVV
                     TPGTITDTGLLASKSDAALAALHFAGKEIGIAWLVLASGDLRATVAAPASLASELARI
                     APSEILIADGARTNVPIDGAALQSVPEWHFDPVRGAQQLRELLGVATLQAFGIDDQRS
                     ILAACGALLTYAQQTQSERLAHVTTLRVERESEFVVLDAATRRNLELTEPLRGDGGPT
                     LFSTLDRCATGMGSRRLRHWLHHPLRDASAAGARHEVIDALLAESRHARVHEVLRNVN
                     DLDRIAARIALRSIRPRELAALRDALPPLRHLRQVLDALDGPLATDLARALELPPAAG
                     ELLARALLTEPAFSPRDGDVIAPGHDAELDELRALRDNAGSFLVELEARERARTGIAN
                     LRVEYNRVHGFYIEVTRAQSDKVPDDYRRRQTMKNAERYITPELKAFEDKALSAQERA
                     RVREKELFDSLVEQLAAHV"
BASE COUNT          693 a         1586 c         1552 g          676 t
ORIGIN      
        1 gcgaccagcc gttcgatctc gcgctgctgc gcggccacgt ggtcgatcag gccgtgcagc
       61 gccgtggtca gcgggtcgtc accgtcctgc gacaggccgt aggcggaaaa acccattcga
      121 ttggcagcct gttcgcgcgc tgcctcgact tccttgtgca ggatgcgcgc gggattgccg
      181 accgcggtcg cacccggcgg cacttccttg atcacgaccg cgttggagcc gacgcgcgcg
      241 ccgtcgccga ccgtgaagcc gccgagaatc ttcgcccccg cgccgacgat cacgccgcgc
      301 ccgagcgtcg ggtggcgttt agcgcccttg gcgagcgacg tgccgccgag cgtgacgccc
      361 tggtaaaggg tgctttcatc gccgatctcg gcagtctcgc cgatcacgat ccccatgccg
      421 tggtcgatga acacgcgccg ccccaccttc gcgccgggat gaatctcgat gccggtgaac
      481 cagcgcgaag cctgcgagat gaagcgcccg agccagcgca gcccgcgcgt ccagcacgcg
      541 tgcgcgagcc ggtgcagcac cagtgcgtgc agtcccgggt aacaggtcag cacctcccac
      601 gtcgagcgcg cggcgggatc ccgctcgaga atgcaggcga tgtcttctcg caggcggctg
      661 aacatgcggt gcgatgtgtg atgaacgggc gctgattcta tcgagcgctc ttacgcccgg
      721 cgcgctcgct cttgcgcgcg atgatggcgg cggcaatgcc gcgcaggatg tcgacctcgg
      781 acgccgtcgg tcgcgcccgc gtcagcagcc gccgcagccg cgccatcaac cgcttcggct
      841 gcgcgggatc gagatagccg agcgcgacca gcgccgcttc gaggtgggca tgcagcgcct
      901 cgaccgcatc gacggtggcc ggcggatcgg ccgcgaagcg cgatgccacc gcgccggcat
      961 cggcgagcgc aagccggcat tcgtacgcca ccacctgcac cgcctgcgcg agattcagcg
     1021 acccatactc gaggtcggtc gggatcgcga cgcacgcgta gcagcgctcg acatcctcgt
     1081 tgcgcagccc gtcgcgctcg gtaccgaaca cgaacgcgat gtcgccatcg agcgcttcca
     1141 cccgcgcttc gccgaccgcc gtgcgcagat cgatcagccg tggaccgaac tcgcgcgcat
     1201 agccggtcat cgcaaacgcg aggcgcactc cggaaagcgc atcggcaagg tctgcatgaa
     1261 ccgtcgcggc acgcagcacg tcgtgcgcgt ggctcgcgaa cgcgagcgcg gcctcgtgct
     1321 cgcgaaagcc cgcgaccttc ggtgcgacga ccgccagcct cgtccagccc atcgtcttga
     1381 gcgcgcgcgc ggccgcgccg atgttgccgg gatggctcgg cgcgacgagc acgaagcgga
     1441 tgcgatcggc aatcgacagt ggcacgagtt cgcgagcatg aaccagtaga atggcggcca
     1501 gtctagcggc gtgcttcagc catccgccgc tgcgcctcct cgacaatcga tcccgatgca
     1561 cccgatgctg aacacggccg tcaaggccgc ccgcaaggct ggcgcgatca tcaatcgcgc
     1621 cagcctcgac ctggacctcg tgcgcgtgtc ggcgaagggc cgcagcgatt tcgtcaccga
     1681 ggtcgatcac gccgccgagg cggcgatcat cgacacgctg aagaccgcgt atccgcagca
     1741 cgcgatcctg gccgaggagt ccggcgccac gaagtcggac accggcgccg actacacgtg
     1801 gatcatcgat ccgctcgacg gcaccacgaa cttcatccac ggctttccgc agtacgcggt
     1861 gtcgatcgca ctccagcacg gcgggcagat cacgcaggcc gtcgtgtacg acccgacccg
     1921 taacgagctg ttcacggcca cgcgcggacg cggcgccttc ctgaacgacc ggcgcctgcg
     1981 cgtgtcacgg cgcgacaagc tgcgcgaggc gctggtcggc accggcttcc cgttccgcca
     2041 gctcgaccac ctcgacgaat acgtgcgcat gttcaagcgc atcaccgagg agaccgcagg
     2101 catccgccgc ccgggcgccg cggcgctcga tctcgcgtac gtcgccgcgg ggcggctcga
     2161 cggattctgg gagttcgggc tgtcgccgtg ggacatggcc gccggcagcc tgctgatcat
     2221 cgaggcgggc ggcatggtcg gcgacttcct cggcgagaag cgctatctgg agacgggccg
     2281 gatcgtctgc ggcgcgccga aggtttacga acagctgctg gcgatggtgc gcgacgtgca
     2341 cctgcaggac gcgcgagcca gggggccgga ggcatgaatc agttcgttga gacgtatctc
     2401 ggcacgcgcc ggcgcgtctg cgccgcggtg ttcatcgtga tcgcggtgct gctgtcgacg
     2461 tcgctttatc tgcaacacgt cgtcggactc gaaccatgcc cgctgtgcat cctgcagcgc
     2521 tacgccttcg tggcggtcgc gctgttcgcg ctgatcgcag cactcgcgcc cgatctgccg
     2581 agcaggatgg cgcacgcggc atcagggctc ttcgcgctgg tcggcggcgg cacggcggcc
     2641 tggcacgtgt ggctgcaggt caacccgccc aagttcgcct cgtgcgggcc ggggctggag
     2701 tacatgctga gcgaattgcc gctggcgcgc gcactgccgc gcatcttcca gggttcgggc
     2761 gactgcaccg aggtgcaatg gaccttcctc ggcctgtcga tcgccggctg gtcgctggtg
     2821 tggttcacca tcctgttcgt cgcgctggtc gccgcgtcgc gccggcgccg ctgaaccgcc
     2881 ctcgcccggc gcagacgtga ctgcggaact gtctcggcac acgccgatga tgcagcagta
     2941 cctgcgcctg aaggcgcagc acccgcgcgc gctgctcttc taccggatgg gggatttcta
     3001 cgagctgttc tatgaagatg ccgagcgcgg cgccaagctg ctgaacatca cgctgacgaa
     3061 gcgcggcacc tcggctggcg caccgatccc aatggcgggc gtgccggtgg tgtcggtcga
     3121 gcagtacctg gcgcggctgg tcaagctcgg tgaatcggtc gcgatctgcg agcagatcgg
     3181 tgaccccgcc accagcaagg ggccggtgga gcgcaaggtc gtgcgcgtcg tcacgccggg
     3241 cacgatcacc gacacgggat tgctcgcgag caagtcggac gcagcgctcg cagcgctgca
     3301 ctttgcgggc aaggagatcg gcatcgcgtg gctggtgctc gccagcggcg acctgcgcgc
     3361 gacggtggcc gcgccggctt cgcttgcctc ggaactggcg cgcatcgcgc cgtccgagat
     3421 cctgatcgcc gacggcgccc gcacgaacgt gcccatcgac ggggcggcgc tccaatcggt
     3481 ccccgagtgg cacttcgatc cggtgcgcgg cgcgcagcaa ctgcgggagt tgctcggcgt
     3541 ggccacgttg caggcgttcg gcatcgacga ccagcggtcc attctcgcgg cgtgcggcgc
     3601 gctgctgacc tatgcgcagc agacgcagag cgagcggctg gcgcatgtga ccaccttgcg
     3661 ggtcgagcgg gaatcggagt tcgtcgtgct cgacgcggca acccgtcgca atctcgaact
     3721 gaccgaaccg ctgcgcggtg acggcggccc gaccctgttc tcgacgctcg accgctgcgc
     3781 gaccggcatg ggcagccggc ggctgcggca ctggctgcac cacccgctgc gcgacgcgtc
     3841 ggcggccggc gcgcgacacg aggtgatcga tgcgctgctt gccgagtcac gccacgcgcg
     3901 cgtgcacgag gtgctgcgca acgtgaacga cctcgaccgc atcgcggcgc gaatcgcgct
     3961 gcggagcatc cgtccgcgcg agctcgccgc gctgcgcgat gcgctgccgc cgctgcgaca
     4021 cctgcgccag gtgctggacg cgctcgacgg cccgcttgcg acggatcttg cgcgcgcact
     4081 cgaactgccg cctgccgccg gcgagttgct ggcgcgcgcg ctgctgacgg agccggcgtt
     4141 ctcgccgcgc gacggcgacg tgatcgcccc cggccatgat gcggagctcg acgagctgcg
     4201 cgcgctgcgc gacaacgccg gcagcttcct ggtcgagctc gaagcgcgcg agcgcgcccg
     4261 caccggcatc gcgaacctgc gcgtcgagta caaccgcgtg cacggtttct acatcgaggt
     4321 cacgcgagcg cagtccgaca aggtaccgga cgactaccgg cggcgccaga cgatgaagaa
     4381 cgccgagcgc tacatcacgc cggagctgaa ggccttcgag gacaaggcgc tgtcagccca
     4441 ggagcgcgcc cgcgtccgcg aaaaggaact gttcgattcg ctggtggagc agctcgcggc
     4501 gcacgtg
//
LOCUS       DBNHBY010000808         3654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1077838, whole genome shotgun sequence.
ACCESSION   DBNHBY010000808 DBNHBY010000000
VERSION     DBNHBY010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1077838"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="AC16HB_18830"
     CDS             <1..697
                     /locus_tag="AC16HB_18830"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR5608902.1"
                     /translation="RGADALWLPAFELAPASDTAAARETLTRLATFDLAVFVSPAAVR
                     ATAVLLNAPWPVVTAIGAVGHATVAAVRTRLRPRDDVRAIAPDQDDEGGSEALWRALD
                     AAQCKPARVLILRAASGREWLADRFAERGAQVEALAVYERRAHVLTGAERARLAQMGA
                     LPVDSVFSSSEAVEAMHALLPAAAWDALLRGTAVGSHTRLAQRLADAGFARVRVAPLR
                     ADEILAVLEESRQ"
     gene            801..1760
                     /locus_tag="AC16HB_18835"
     CDS             801..1760
                     /locus_tag="AC16HB_18835"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR5608903.1"
                     /translation="MPWVALGIFAIAGVVAYRDLAHGNAELREQVAQMARDTAPAVAQ
                     ARKDAQDAQRQVAELKQTVETMQADRGAIDQLVGDVVRMRDDAALIDIERLITLASAE
                     LQISGHVPTALAALQAADLRLMQVERPQYVGLRRALTRDIDRLRAVPQVDFTGLALKI
                     DQLVQGVDGWPLLSDAKLARAAAATKSAPPPPPPQGVWDRLRAWAALEFGDLVRIREV
                     ETPEALLLSSNQQQLVRERLKLRLLSARQALLARNDKLFRSDLAESQAIVNRYFDARA
                     SAPAAALAQLRQIAQTTLAVDVPSLAESLAALRALRSAPPPKR"
     gene            1757..2989
                     /locus_tag="AC16HB_18840"
     CDS             1757..2989
                     /locus_tag="AC16HB_18840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR5608904.1"
                     /translation="MKSLIWALLLALVAAMLAMAAQFNDGNVVVLLPPYRIDLSLNLF
                     LLLSVVFALVVYVIAFVAHRAFDFPRQVSLYRERRELLGGLRALKDSVQALLEGRFAR
                     AERAAKTAQASTDSAGLAALVGARAAHRMQEYDRRDAWLQAAASDRSVQTARLVSSAE
                     MWSESRENERALEAIRGVQAGGSRHIHAARIALAANAQAGRWEELLKNVRALAKRNAL
                     HELLVARYKRVGYRARLMEKRHDAGLLEAEWSRIPPEDRREPGLAADAARWLAAVERG
                     ASAAMLLEDALEHEWDPRPIEAYGQIDAPPYRERIEKCEAWLQDHPNDAVLLRALGRL
                     CMREQLWGKAAQYLRQSAAILPHPDTSIARAQLAELTDDAAAAHEHYRDAALAYARLA
                     RTVERDSAARIARRDASL"
     gene            3056..>3654
                     /gene="dctP"
                     /locus_tag="AC16HB_18845"
     CDS             3056..>3654
                     /gene="dctP"
                     /locus_tag="AC16HB_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR5608905.1"
                     /translation="MKLHRTLFLAGLLAAALNAQAQTTIKIGYATSPTSHDGVGTNAF
                     CEEVEKRTSGRYKCQQYPNSALGGEREMIEAVQLGTLDVVNTSTGPIGNFVPEVNIVD
                     IPFLFRDYDHARKVMDGPIGDELRAKFPPKGLIALAWTENGFRHLTNSKRAVNTPDDV
                     KGLKVRTMQNKVHIQAFQSIGVQPTPMAFPELFTALQQGT"
BASE COUNT          540 a         1245 c         1341 g          528 t
ORIGIN      
        1 gcgcggcgcc gatgcgctgt ggttgccggc gttcgagctg gcgccggcgt ccgatactgc
       61 cgcggcgcgc gaaacgctga cacgactggc aacgttcgat ctggcggtct tcgtcagccc
      121 ggccgcagtg cgcgcaaccg cggtgctgct gaatgcgccg tggccggtgg tgactgcaat
      181 cggcgccgtt ggccatgcga ccgtcgcagc ggtgcgcaca cggctgcgac cacgcgacga
      241 tgtgcgcgcg atcgcgcccg accaggacga cgaaggcggc tccgaggccc tgtggcgtgc
      301 cctcgatgct gcgcaatgca agccggcgcg cgtgctgatc ctgcgcgcgg cgtcgggccg
      361 cgagtggctc gccgatcgat tcgccgagcg cggcgcgcag gtcgaggcac tcgcggtgta
      421 cgagcgccgc gcgcacgtgc tcacgggagc ggagcgcgcg cgcctggcgc agatgggggc
      481 gttgccggtc gacagcgtgt tctccagcag cgaggcggtc gaggcgatgc atgcgctgct
      541 gcccgccgcc gcgtgggatg cgctgctgcg tgggaccgcg gtcggatcgc acacacgcct
      601 tgcgcagcgg ctggcggacg ccggcttcgc gcgcgtgcgc gtggcgccgc tgcgcgccga
      661 cgagatcctg gccgtgctcg aagagtcgcg tcagtagaat cgcgcgcacg gcgcgccttg
      721 cgccgcttgc cgcgatgaac gcgcctccgc ctccttcttc gacgtcagcc gccgcggcgc
      781 ggccgggtcg cgcgcgtttc gtgccgtggg tcgcgctggg catctttgcg attgccggcg
      841 tggtcgccta tcgcgacctc gcgcacggca acgcggagct gcgcgaacag gtcgcgcaga
      901 tggcgcgcga caccgcaccg gcggtggcgc aggcgcgcaa ggatgcacag gacgcacagc
      961 ggcaagtggc ggagctgaag cagacggtcg agacgatgca ggccgaccgg ggcgcgatcg
     1021 atcagctggt tggcgacgtg gtgcgaatgc gcgacgatgc ggcgctgatc gatatcgagc
     1081 gtctgatcac gctggcgtcg gccgaactgc agatctccgg ccacgtgccc acggcactcg
     1141 ccgcactgca ggccgccgac ctgcggctga tgcaggtcga gcggccacaa tacgtcgggc
     1201 tgcggcgggc gctgacgcgc gacatcgatc ggctgcgcgc ggtgccgcag gtcgatttca
     1261 cggggctggc gctgaagatc gaccagctcg tgcagggtgt ggacggctgg ccgctgttgt
     1321 cggatgcgaa actggcgcgc gccgcggccg cgacaaagag cgcgccgccg cctccgccgc
     1381 cgcagggtgt gtgggaccgg ctgcgcgcgt gggcagcgct ggagttcggc gatctcgtcc
     1441 gcattcgcga ggtggagacg cccgaggccc tgctgctgtc gtccaaccag cagcaactgg
     1501 tgcgcgagcg gctcaagctg cgcctgcttt cggcgcggca ggcgctgctg gcgcgcaacg
     1561 acaagctgtt ccgcagcgat ctggccgaga gtcaggccat cgtcaaccgc tatttcgacg
     1621 cgcgcgcctc ggcgcccgcg gccgcgctcg cgcaactgag gcagatcgcg cagaccacgc
     1681 tcgccgtcga cgtgccgtcg ctcgccgaaa gcctggccgc gctgcgagcg ttgcgcagcg
     1741 cgccgccgcc caagcgatga agtcgctgat ctgggccctg ctgctggcct tggtcgcggc
     1801 catgcttgcg atggccgcgc agttcaacga cggcaacgtg gtggtgctgc tgccgccgta
     1861 ccggatcgac ctgtcgttga acctcttcct gctgctgtcg gtggtgttcg cgctcgttgt
     1921 ctacgtgatc gcgttcgtcg cccatcgtgc gttcgacttc ccgcgccagg tgtcgctcta
     1981 tcgcgagcgg cgcgagctgc tcggcgggct gcgcgcattg aaggattcgg tgcaggcgct
     2041 gctcgagggc cgcttcgcgc gcgccgagcg ggccgcgaag accgcgcagg cttcaaccga
     2101 cagcgcgggg ctggcggcgc tggtcggcgc gcgcgccgcg caccgcatgc aggagtacga
     2161 ccgccgcgat gcgtggttgc aggctgccgc gtccgatcgc agcgtgcaga ccgcgcgcct
     2221 ggtgtcgagc gcggagatgt ggtccgagag tcgcgagaac gagcgcgcgc tggaggcgat
     2281 ccgcggcgtg caggcgggcg gcagccggca catccacgcc gcacgcatcg cgctggcggc
     2341 caacgcgcag gcggggcgct gggaggaact gctgaagaac gtgcgcgcgc tcgccaaacg
     2401 caatgcgctg cacgaactgc tcgttgcgcg ctacaagcgc gtcggctatc gcgcgcggct
     2461 gatggagaag cgccatgacg ccggcctgct cgaggccgag tggagccgca tcccccccga
     2521 ggaccggcgc gagccggggc tggcggccga cgcggcgcgc tggctagccg cggtcgagcg
     2581 cggcgccagc gcggccatgc tgctcgagga cgcgctggag cacgaatggg acccgcggcc
     2641 gatcgaagcc tacggacaga tcgacgcgcc gccgtaccgc gagcggatcg agaagtgcga
     2701 ggcctggctg caggatcacc cgaacgatgc ggtgctgttg cgcgcgctgg gccggctatg
     2761 catgcgcgaa cagttgtggg gcaaggccgc gcagtacctc cgccaaagcg ctgcgatcct
     2821 gccgcatccc gacacgtcga tcgcgcgcgc gcagcttgcc gaactcaccg atgatgcggc
     2881 cgccgcgcac gagcactatc gcgacgcggc gctcgcctac gcgcggctgg cgcgcacggt
     2941 cgaacgcgat tcggccgcgc gcatcgcgcg gcgcgacgcg tcgctttgat gcgctcggct
     3001 tgacaagcac ggacccgctg cggagacttc cacggcttcg gacgaaggag acacgatgaa
     3061 gttgcatcgc acgcttttcc tggcgggact gctcgcggcg gcgctgaacg cgcaggcgca
     3121 gaccacgatc aagatcggct acgccacctc gccgacctcg cacgacggcg tcggcaccaa
     3181 tgcgttctgc gaggaagtgg agaagcgcac gtccggccgc tacaagtgtc agcagtatcc
     3241 gaactcggcg ctcggcggcg agcgcgagat gatcgaggcg gtgcagctcg gcacgctcga
     3301 cgtcgtcaac acctcgacgg gcccgatcgg caacttcgtg ccggaggtca acatcgtcga
     3361 catcccgttc ctgttccgcg actacgacca cgcgcgcaag gtcatggatg gtccgatcgg
     3421 tgacgaactg cgcgccaagt tcccgcccaa gggcctgatc gcgctcgcgt ggaccgagaa
     3481 cgggttccgg cacctgacca acagcaagcg cgcggtcaac acgcccgacg acgtgaaggg
     3541 tctgaaggtg cgcacgatgc agaacaaggt gcatatccag gcattccagt cgatcggcgt
     3601 gcagccgacg ccgatggctt tcccggagct gttcaccgcg ctccagcagg gcac
//
LOCUS       DBNHBY010000809         1753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_671867, whole genome shotgun sequence.
ACCESSION   DBNHBY010000809 DBNHBY010000000
VERSION     DBNHBY010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_671867"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="AC16HB_18850"
     CDS             complement(<1..749)
                     /locus_tag="AC16HB_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608906.1"
                     /translation="MLNKILFGSPLHEHAEPAQRMLGVAELAPDSPALVQLLAADPAP
                     EVRSAAAARCTDLAALAAAWRSETAPDVRTAIAASLGCAVAATDDAAAAHALLAAPEC
                     NDVIRVQVALHALDEARRRTAIEGIRDENLLVDVALDAAHAPLRLAAAERVHAPAALR
                     RMYEAARDKDRGVARLARQRLDALHQRETKAAAADELIAQAEALAAQPGHILTAAIEL
                     DRRWKALGLTDDPDRSARWEAVGQVMQQRFAR"
     gene            complement(779..>1753)
                     /locus_tag="AC16HB_18855"
     CDS             complement(779..>1753)
                     /locus_tag="AC16HB_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR5608907.1"
                     /translation="LIEANLTGHDSHGIGMLPRYVDAYREGGLKPNAHVATVLDAGAL
                     LRLDGGAGFGQVIGREAMALGIARARELGSSIVALGNSHHLGRIGAWAEQCARAGLVS
                     LHFVNVISRAIVAPFAGSDARFGTNPFCAGVPLAGREPVILDFATSVLAQGKMRVAYN
                     KGESAPPDTLLDERGHPTQDPKFAVVPPFGAIVAFGLHKGSGLAVMCELLGGALAAGV
                     TQRTDDASKRRVLNGMLSVLIDPARLGDRDAFERETLAFLDWVKASPPRMGFDRVRIA
                     GEPEREARAQRAAHGVPVDTTTWHEILAAAAKLGVDPRTVDAVAGVRG"
BASE COUNT          228 a          640 c          638 g          247 t
ORIGIN      
        1 cgtgcgaagc gctgctgcat cacctgtccc accgcctccc agcgcgcgct gcggtcggga
       61 tcgtcggtga ggccgagcgc tttccagcgc cgatcgagtt cgatcgcggc ggtcaggatg
      121 tgccccggct gcgcggccag cgcctcggct tgcgcgatca actcgtcggc cgcggctgct
      181 ttcgtttcgc gctgatgaag cgcatcgagc cgttggcgcg ccagccgcgc cacaccgcgg
      241 tccttgtcgc gtgcggcttc gtacatccgc cgcaatgccg cgggcgcatg cacgcgctcg
      301 gctgccgcca gccgcagggg cgcgtgcgcg gcatcgagcg cgacgtcgac cagcaggttc
      361 tcgtcgcgga tgccttcgat cgcggtgcgc cggcgcgctt cgtccagcgc gtgcaacgcc
      421 acctgcacgc ggatcacgtc gttgcactcg ggcgcggcga gcagcgcgtg cgcggcggcc
      481 gcgtcgtcgg tcgccgcgac cgcgcaaccg agggacgccg cgatcgccgt gcgcacgtcg
      541 ggcgcggtct ccgaccgcca tgccgcggcc agcgcggcga gatcggtgca gcgcgcggcc
      601 gcggcgctgc gcacctcggg cgcgggatcc gcggcgagca gttgcacgag tgccggcgag
      661 tcgggcgcca gttcggccac gccgagcatg cgctgggcgg gctcggcgtg ctcgtgcagc
      721 ggggaaccaa acaggatctt gttcagcacg ggcgcgatgg taccgagttg cgtcgccctc
      781 agccgcgcac gccggcgacg gcgtccacag tgcgcggatc gacgccgagc ttggccgcgg
      841 cggcgaggat ttcatgccac gtggtcgtgt cgaccggcac gccgtgcgcg gcgcgctgcg
      901 cacgcgcctc gcgctccggc tcgccggcga tgcgcacccg gtcgaagccc atgcggggcg
      961 gggacgcctt gacccagtcg aggaacgcca gcgtctcgcg ctcgaacgcg tcgcgatcgc
     1021 cgagccgcgc cgggtcgatc agcacgctca gcatgccgtt gagcacgcgc cgtttgctgg
     1081 catcgtcggt gcgctgcgtg acaccggcgg cgagcgcgcc gccgagcagt tcgcacatca
     1141 ccgccaggcc cgagcccttg tgcagcccga acgcgacgat cgctccgaac ggcggcacga
     1201 ccgcgaactt cgggtcctgc gtcgggtgac cgcgctcgtc gagcagggtg tcgggcggcg
     1261 ccgactcgcc cttgttgtag gccacgcgca tcttgccctg cgccagcacg ctggtcgcga
     1321 aatcgaggat gaccggctcg cgtccagcaa gcggcacgcc ggcgcagaac ggattcgtgc
     1381 caaagcgcgc gtccgagccc gcgaacggcg cgacgatcgc gcgcgagatc acgttgacga
     1441 agtgcagcga caccaggccg gcccgggcgc attgctcggc ccatgcgccg atacgcccga
     1501 ggtgatgcga gttgccgagc gctacgatgc tgctgccgag ttcgcgcgcc cgcgcgatgc
     1561 cgagcgccat cgcctcgcgg ccgatcacct ggccgaagcc ggcgccgccg tccaggcgca
     1621 gcagcgcgcc ggcatcgagc acggtcgcga cgtgcgcgtt cggtttcagt ccgccttcgc
     1681 ggtacgcgtc gacgtagcgc ggcagcatgc cgatgccgtg cgagtcatgg ccggtcaggt
     1741 tcgcttcgat cag
//
LOCUS       DBNHBY010000810         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_334958, whole genome shotgun sequence.
ACCESSION   DBNHBY010000810 DBNHBY010000000
VERSION     DBNHBY010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_334958"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..516)
                     /locus_tag="AC16HB_18860"
     CDS             complement(100..516)
                     /locus_tag="AC16HB_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG4315 family predicted lipoprotein"
                     /protein_id="HIR5608908.1"
                     /translation="MKRPALLAALALAAFAAHANPTTERNGLLVNKEGRTLYTFTKDA
                     AGKSNCNGGCATAWPPFLAANPALAGGDFSIVARDDGARQWAYKGMPLYFFAADQQPG
                     DMKGDGQGGVWFTARTKSGSANTAEQDYASSYSNKY"
     gene            complement(635..1378)
                     /locus_tag="AC16HB_18865"
     CDS             complement(635..1378)
                     /locus_tag="AC16HB_18865"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5608909.1"
                     /translation="MNYNRPELLDRLASEYVLGTLRGRARRRFEQVLLSQPAARAAVG
                     EWQHRLAQLAEPVPAVVPPARVWRAIDARTAAGPSGAKSRTRANWWSTLWKPALGFAF
                     GAAVTVGIVQLNPALMVSLDRLAQREQALPQSYVGLLTDRDNQPTLLVSSTRHGARVS
                     VKFLRPVTVPAGKVIQVWALPKEGPAFPLGVAAPATPPASSAFEMSASAEKLLSAVPR
                     LAVSFEDQPAGAGATPGEFVLSGFCVKLW"
     gene            complement(1375..1959)
                     /locus_tag="AC16HB_18870"
     CDS             complement(1375..1959)
                     /locus_tag="AC16HB_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5608910.1"
                     /translation="MTRDASPSDQSRELQAQLARVALGDKAAFARVYQMTSSHLFGIA
                     LRILNRRDAAEDVLQEAFVSVWHQAASYQAATSQPMTWLITIVRNKALDVVRSAAARR
                     ETEMPVDEEGQAMDVADDRPNPLSLLEQAADALGIRRCMAALDAAHRQCLALAYYHGM
                     SHSEVAEHVNAPLGSVKAWIRRGLERLKTCLAGA"
BASE COUNT          336 a          730 c          702 g          345 t
ORIGIN      
        1 gccgccagtt catcgaaatc aacgcgctcg cggccgggaa catcgacgct tgaaccggga
       61 gacgactgcg cgtcgccccc ggcacgcacg gcgcagcgtc taatacttgt tcgagtagct
      121 gctcgcgtag tcctgttcgg cagtgttcgc cgagcccgat ttcgtccttg cagtgaacca
      181 cacgccgcct tgcccgtcgc ccttcatgtc gccgggctgc tgatcggccg cgaagaaata
      241 cagcggcatg cccttgtaag cccactgccg ggcgccgtcg tcgcgcgcga cgatcgagaa
      301 gtcgccgccg gcaagcgccg gattggcggc caggaacggc ggccacgccg tggcgcaacc
      361 gccattgcag ttgctcttgc cggctgcgtc cttggtaaac gtgtacagcg tcctgccctc
      421 tttgttgacc agcaggccgt tccgttcggt ggtcgggttg gcgtgcgcgg caaacgcggc
      481 gagcgcaagg gcagccagaa gggcgggacg tttcatgatc tctccgtgag tgggttgatg
      541 agctgaatgc gctcacgggg ccacttacgg gatcggggtg agatcggatg caggacgcgc
      601 gaacggctgc gcggcgcgcg ctgcccgtgc gttgctacca gagcttcacg cagaacccgg
      661 acagaacgaa ctcgcccggg gttgcgccgg cgccagccgg ctggtcttcg aacgaaaccg
      721 ccagccgtgg caccgccgac agaagcttct cggcgctggc actcatctcg aacgcggaac
      781 tcgccggcgg tgtggccggg gcggcgacgc cgagcgggaa ggcggggcct tccttcggca
      841 gcgcccacac ctggatcacc ttgccggccg gcaccgtcac cggccgcaag aacttcaccg
      901 atacgcgcgc gccgtgacgc gtggagctga cgagcagcgt cggctggttg tcgcggtcgg
      961 tcagtagccc gacatagctt tgcggcagcg cctgctcgcg ctgcgccaga cggtcgagcg
     1021 acaccatcag ggccgggttc aactgcacga taccgacggt gacggcggcg ccgaaggcga
     1081 atccaagcgc cggcttccac agcgtcgacc accagttcgc ccgcgtccgt gactttgcgc
     1141 ctgatggccc ggccgcggtg cgcgcgtcga tggcgcgcca gacgcgcgcg ggcgggacga
     1201 cggccggcac aggctccgcg agctgcgcca ggcgatgctg ccactcgccc accgccgcac
     1261 gcgccgccgg ctgcgacagc agcacctgct cgaagcgccg gcgggcacgc ccgcgcagcg
     1321 tgccgagcac gtattcggac gccagtcgat cgagcaactc gggccggttg taattcatgc
     1381 acccgccaga caggtcttca atcgttccag tcctcggcgt atccatgcct tgacgctgcc
     1441 cagcggagcg ttgacgtgct cggccacctc cgaatgcgac atgccgtggt agtaggccag
     1501 cgccagacac tgccgatgcg ccgcatcgag tgccgccatg cagcggcgga tgccgagcgc
     1561 atccgcagcc tgttccagca ggctcagcgg gttcggccgg tcgtccgcga cgtccatcgc
     1621 ctgcccttcc tcgtcgaccg gcatctcggt ctcccgtcgc gccgcggcac tgcgcacgac
     1681 gtcgagcgcc ttgttgcgga cgatcgtgat caaccaagtc atcggctggc tggtggcggc
     1741 ctgatagctg gcggcctggt gccagacgct gacgaatgct tcctgcagca catcctccgc
     1801 cgcatcgcgc cgattcaaga tacggagcgc aatgccgaac agatgcgacg aggtcatttg
     1861 atacacgcgc gcgaacgctg ccttgtcccc gagcgccacg cgcgccagtt gcgcctgcag
     1921 ttcgcggctc tggtccgacg gcgaggcgtc gcgtgtcatg ccgcagcgat cagcgccgta
     1981 tcggcacgaa gataggtggg aaacgtcgtc gtcatcgtgc ggtctttcgt tgcctgatcg
     2041 tgccggcggt cgcggcaggg aggcttgcca ccgtttctac ccgtggtcac ccaccctgtc
     2101 gaatcccaga aaa
//
LOCUS       DBNHBY010000811         2840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1689680, whole genome shotgun sequence.
ACCESSION   DBNHBY010000811 DBNHBY010000000
VERSION     DBNHBY010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1689680"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16HB_18875"
     CDS             complement(<1..469)
                     /locus_tag="AC16HB_18875"
                     /inference="COORDINATES: protein motif:HMM:NF015642.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HIR5608911.1"
                     /translation="MSAPIAYDDVAQALAAGKSTVSAAEAHGCLVGALCARRVYLPAE
                     WMEELLPEEGAAAATPPLQALFDHSRAALEATDLEFEPLLPADDTPLSVRVEALGAWA
                     QGFLYGFGSAGPLPKGSLPGDIAEILSDFAEVSRAGAVGSESAEVEESALAELV"
     gene            561..791
                     /locus_tag="AC16HB_18880"
     CDS             561..791
                     /locus_tag="AC16HB_18880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HIR5608912.1"
                     /translation="MNEPAARSAVDVELRRIERRLEELVATVTQLKEENRALRQRQDS
                     LIMERANLLQKNEQVRARVEAMIGRLKAMEQG"
     gene            791..1108
                     /locus_tag="AC16HB_18885"
     CDS             791..1108
                     /locus_tag="AC16HB_18885"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR5608913.1"
                     /translation="MSEERISRVSVRLLDREYQVACPAEERSDLLDSAEYLDGKMREI
                     RDSGKVVGLDRIAVIAALNIANELIKQRKHGTVVEGDLGSRLKSLRERMESALEKGQQ
                     LEL"
     gene            1143..1327
                     /gene="ssrS"
                     /locus_tag="AC16HB_18890"
     ncRNA           1143..1327
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16HB_18890"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1399..1998
                     /locus_tag="AC16HB_18895"
     CDS             1399..1998
                     /locus_tag="AC16HB_18895"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR5608914.1"
                     /translation="MRRELRALRRSLPEPVREAAEAAIRRELSRLGVWRRGRRVAAYL
                     AMPGEVGLAAALADGLRRGAHIYVPRIASRRGATLAFVPLDAGAALRRNVYGIAEPAA
                     RAARLPVRCLDDVLLPLVGFDAAGHRLGMGAGYYDRALWHRATRNAGWRRPRLIGIAY
                     SLQQVEHIAAQPWDVPLDLVVTERGVLRCSHAPDQEPHR"
     gene            1995..2465
                     /locus_tag="AC16HB_18900"
     CDS             1995..2465
                     /locus_tag="AC16HB_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR5608915.1"
                     /translation="MKNHWLLKSEPDTFSVDDLARAPRRTTGWEGVRNYQARNFLRDD
                     FGKGDLGFFYHSSCAEPGIAGIVEVVRSGYPEPTQFDPRSDYYDAAATRDAPRWYTVD
                     VRLAQRVEPVISLSELREHASGALRDMLLLKRGNRLSVTPVSAAEWRFITALRR"
     gene            complement(2580..>2840)
                     /locus_tag="AC16HB_18905"
     CDS             complement(2580..>2840)
                     /locus_tag="AC16HB_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608916.1"
                     /translation="VLAGHALLTPETTVPACAGTVVVSDSAAVFLAKDRRLFFKRPYF
                     FFAAFFLVAFFLVAFFALAAMINLHVGLVPGAVFTEFIFQLQ"
BASE COUNT          482 a          967 c          973 g          418 t
ORIGIN      
        1 ccaccagttc ggccagcgcg gattcctcga cctcggcgct ctccgagccg accgcgccgg
       61 cacgcgagac ctcggcgaaa tccgacagga tctcggcgat gtcgcccggc agcgatccct
      121 tgggcagggg gcccgcggag ccgaagccgt acaggaagcc ctgcgcccag gcgccgagcg
      181 cctcgacgcg caccgagagc ggcgtgtcgt cggccggcag cagcggctcg aattccaggt
      241 cggtcgcctc gagcgcggcg cgactgtggt cgaacagcgc ctgcagcggt ggcgtggccg
      301 cggctgcgcc ttcctcgggc agcagttctt ccatccactc ggccggcagg tagacgcggc
      361 gcgcgcacaa ggcaccgacc aggcagccgt gggcctcggc ggcactcacg gtcgacttgc
      421 cggcggcaag cgcctgcgcg acgtcgtcgt aggctatcgg tgcggacatc tgcgtatcgt
      481 accaccgcgc ctttcgccgt cgcggcacgg gccattgagt gaaattgatt ccccccggtg
      541 cgggctctat agttcctcgc atgaacgagc ctgccgcacg ctccgccgtc gacgtcgagc
      601 tgcgccgcat cgagcggcgc ctcgaggagc tggtcgccac ggtgacccag ctcaaggaag
      661 agaaccgtgc gctgcgccag cgtcaggaca gcctgatcat ggagcgcgcc aacctgctgc
      721 agaagaacga gcaggtgcgt gcgcgggtcg aggcgatgat cggccgcctc aaggccatgg
      781 agcagggctg atgagcgaag agcgcatctc gcgggtgagc gtgcggttgc tggaccgcga
      841 ataccaggta gcctgcccgg ccgaggagcg gtccgacctg ctcgactccg ccgagtacct
      901 cgacggcaag atgcgcgaga tccgcgattc aggcaaagtg gtcgggctcg accgcatcgc
      961 cgtgatcgcc gcgctcaaca tcgcgaacga gctgatcaag cagcgcaagc acggcacggt
     1021 ggtcgagggc gacctcggct cgcgcctgaa gtcgctgcgc gagcgcatgg agtcggcgct
     1081 cgaaaaaggt cagcaactgg aactctgagg gttcgcacta gtccgaacct tcgacgattt
     1141 ggcgccgcct gcggtgttcg acacggtccg ggttacctct cgaccgtatg ttaaataccc
     1201 aggggagtcc ggccagcggc aacactgtgc aggtccgcct cgagcgggaa gccttacttg
     1261 ccgcggtctt ccccgaatgt tgctctatgt cgagagcaat ggccgtgacg gcacaggcgg
     1321 gtggcgcctc tcccctcttc ccaacgcgcg gcaacgccgc gccgtgccgt gctgagccct
     1381 gacgcacggc gcgcgcagtt gcgccgcgag ctgcgggcgc tgcgacgcag cctgcccgag
     1441 cccgtccgcg aggcggccga agccgccatc cgccgcgaat tgagccgcct cggcgtctgg
     1501 cgccgggggc ggcgcgtcgc ggcctacctg gccatgccgg gcgaagtggg cctggccgcc
     1561 gcactggcgg acgggctgcg ccggggtgct catatatatg tgccccgtat cgcgagccgc
     1621 cgcggcgcga ccctggcctt cgtcccgctc gacgcgggcg ccgcgctgcg ccgcaatgtc
     1681 tacggcatcg ccgagcccgc cgcgcgtgcc gcgcgcctgc cggtgcgctg cctcgacgac
     1741 gtgctgctgc cgctggtcgg cttcgacgcg gcgggccatc gtctcggcat gggtgcgggt
     1801 tactacgatc gcgcgctctg gcaccgcgcg acacgcaacg ccggctggcg acggccgcga
     1861 ctgatcggca tcgcgtattc cctgcagcag gtcgagcaca tcgcggcgca gccgtgggac
     1921 gtcccgctcg acctcgtcgt caccgagcgc ggggtgctgc gttgctcgca cgcgcccgac
     1981 caggagcccc accgatgaag aaccactggc tgctcaagtc cgagccggac accttctccg
     2041 tcgacgatct cgcgcgcgcg ccgcggcgca cgacgggctg ggagggggtc cggaactacc
     2101 aggcgcgaaa ttttcttcgc gacgatttcg gcaagggcga cctcggcttc ttctaccact
     2161 cgagctgcgc ggagccgggc atcgccggca tcgtcgaagt ggtgcgctcc ggctatccgg
     2221 aaccgacgca gttcgatccg cgcagcgact actacgacgc agcggcgacg cgcgacgcgc
     2281 cgcgctggta cacggtcgac gtgcggctcg cacaacgcgt agagcctgta atttccctgt
     2341 cggaactgcg cgagcacgcg agcggcgcgt tgcgcgacat gctgctgctg aagcgcggca
     2401 accggctctc ggtgacgccg gtgagcgccg ccgagtggcg cttcatcacc gcgctgagac
     2461 gctgagtcga cgctcgtcga gcgacgcgaa tcgcatcgca gcacgacgct cgcgacgcgt
     2521 cgtcgcgacc tcgacgccgg tcacgcccgc ggagcgcgta tgaaaaaatc gtgacgctgc
     2581 tattgcaatt ggaaaatgaa ttctgtaaat actgcgcccg ggactaaccc gacatggaga
     2641 ttgatcatgg cagctaaggc aaagaaggcg accaagaaga aggccaccaa gaagaaggcg
     2701 gcgaagaaga agtaaggtcg cttgaagaag aggcggcgat ccttcgcaag aaagaccgcc
     2761 gccgaatctg aaacgacgac tgtgcccgcg caagcgggca cagttgtttc aggggtcagc
     2821 aaagcatggc cagccaggac
//
LOCUS       DBNHBY010000812         7398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1078431, whole genome shotgun sequence.
ACCESSION   DBNHBY010000812 DBNHBY010000000
VERSION     DBNHBY010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1078431"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..301)
                     /locus_tag="AC16HB_18910"
     CDS             complement(92..301)
                     /locus_tag="AC16HB_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608917.1"
                     /translation="MKRRVSVKEHAPTVLILLAALVCHSLNLDGAGLALFFGAAVQEL
                     WLWLRSVQRPARPHGRAALMLRNHR"
     gene            complement(298..1191)
                     /locus_tag="AC16HB_18915"
     CDS             complement(298..1191)
                     /locus_tag="AC16HB_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR5608918.1"
                     /translation="MPEPTPGRIAAHQNHKSLLERLTAFIFREPENREELLEALHDAH
                     ERNLLDADALSMIEGVLQVSELRARDLMVPRSQIDMIDIDEPPQKWIPHVIETAHSRF
                     PVFEGSRDHIIGVLLAKDLVRYYHDEDFDVRSMLRPAVFIPESKPLNVLLRDFRANRN
                     HMAIVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEEADAIVAESDGRYRVKALAEI
                     AAVNAMFGTELPEAEFDTIGGLVTDQLGRVPKRGDAIELDGLRFEVLRADARQAHLFQ
                     VSRLPKTVAREFEELEARQGS"
     gene            complement(1347..1784)
                     /gene="ybeY"
                     /locus_tag="AC16HB_18920"
     CDS             complement(1347..1784)
                     /gene="ybeY"
                     /locus_tag="AC16HB_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR5608919.1"
                     /translation="MNRLSLSVQGDEAFADLPARRTLRRWIATALERDARLTLRFVGE
                     REGRQLNRRFRGQDHATNVLTFDYAQRPLIDADIVICVPVVRREARAQRRPLRSHLAH
                     LVVHGTLHAQGYDHQRRVDARRMERREIALLDACGIPNPYDEG"
     gene            complement(1781..2800)
                     /locus_tag="AC16HB_18925"
     CDS             complement(1781..2800)
                     /locus_tag="AC16HB_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR5608920.1"
                     /translation="MAKRATAKKVLAWAADIDNSQLSNLTGALDSNLRQIETELDVAI
                     RRRGHQFRIEGAPAPAQQAVSVLGHFVSRAQRPISIDDIQLYFVEMRGGRTREPLVEP
                     EGEPQPQLHTRRRDLHGRTPRQRDYIEAILAHDISFSIGPAGTGKTYLAVACAVDALE
                     RDAVKRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALFDLLGFEKTQRLLEKQ
                     TIEIAPLAYMRGRTLNHAFVILDEAQNTTPEQMRMFLTRIGFGSKAVVTGDVTQIDLP
                     RGSSSGLIEAQHILRGVRGIAFTYFTAADVVRHPLVARIVEAYERAEKDNPSPPRGRR
                     QVAPE"
     gene            complement(2802..4157)
                     /gene="miaB"
                     /locus_tag="AC16HB_18930"
     CDS             complement(2802..4157)
                     /gene="miaB"
                     /locus_tag="AC16HB_18930"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR5608921.1"
                     /translation="MTKKLYIRTFGCQMNEYDSAKMADVLNEASGLQPTDRPDDADII
                     LFNTCSVREKAQEKVFSDLGRVKHLKRRNPNLVIGVGGCVASQEGAAIVARAPYVDVV
                     FGPQTLHRLPQLIERRRVTGTPQVDISFPEVEKFDHLPPPRVEGASAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPFDDVLTEVADLADHGVKEITLLGQNVNAYLGRMRDGQTADL
                     ALLLDVVHEVPGIERIRYTTSHPKEFTPRLIDAYARLPKLVGHLHLPVQAGSDRMLAA
                     MKRGYTVLEYKSIIRRLRAVRPGISISSDFIVGFPGETEHDFERTLKLVSEIGFDASF
                     SFVYSRRPGTPAAELPDATPQEVKLERLQRLQALLSEQQQRISAVMVGTRQRILVEGP
                     SKKDPDELMGRTENNRIVNFDAGGRAIELSGRMVDVRITAALPHSLRGEQVVRDAPLA
                     V"
     gene            complement(4204..4509)
                     /locus_tag="AC16HB_18935"
     CDS             complement(4204..4509)
                     /locus_tag="AC16HB_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608922.1"
                     /translation="MPRWTVAVIALCFSVAASGQVMLRTLPADAKRGKLTAIELGSVR
                     IDGKTYRMSPGARILNANNMTVTPNQVAADTVIRYQLDGQGQVRTIWMLTPEEAKRR"
     gene            complement(4525..4601)
                     /locus_tag="AC16HB_18940"
     tRNA            complement(4525..4601)
                     /locus_tag="AC16HB_18940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4565..4567),aa:Met,seq:cat)
     gene            complement(4679..6292)
                     /locus_tag="AC16HB_18945"
     CDS             complement(4679..6292)
                     /locus_tag="AC16HB_18945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5608923.1"
                     /translation="MPDDDRRASAGHGNTVPTARRAGLRAIASFVLRGHRRGEAVPAS
                     PPAAPPQLFVNPLRLSNEQTSLFGEILDWMLAPLLLLWPMSVTITYLVAQSIADAPYD
                     RTLAENVELLSDYVKEHRGQVSLQLPIPAHDVLRRDSGESVNYMVLGLRGEFVAGERE
                     VPLPPEDETPAPGLIRLRFDQMRGAEVRVAYTWVAFPRATGQPALVQVAETLADRSQL
                     ANDIIRGVIIPQFIVLPIAVTLVWFALTRGLAPLNSLQGRIRARRPDDLSPIDPKAAP
                     EELGPLVNSFNELLERLKRSMQTQTRFLADAAHQMKTPLAGLRTQAELALRETDPQQL
                     RRSLRQIAESSERAAHLIDQLLALARTEHQAADLGAFEIVDLATLARKLVQEFVPLAL
                     ARSIDLGFEGPETATTATRIIGMPVALREMLKNLVDNALRFTPGGGTVTVRVEALPAA
                     VRLEVEDTGPGIPEADRALIFERFYRVLGTQVDGSGLGLAIVREIVEQHDALLHVGSN
                     PNSRAADGPGTRMSVEFRHIAGAPSTVRL"
     gene            complement(6346..7056)
                     /locus_tag="AC16HB_18950"
     CDS             complement(6346..7056)
                     /locus_tag="AC16HB_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5608924.1"
                     /translation="MRILIAEDDPVLADGLSRSLRAGGYAVDVVSDGEAADTALSAQQ
                     FDLLILDLGLPKLPGLEVLRRMRARQTATPVLILTAADSVEQRVKGLDLGADDYMAKP
                     FALSELEARARALMRRAMGGAQTLLRHGPLTLDQVGRVASLNDKPLELSGRELGLLEI
                     LLSRPGRLVSKEHLVDHLCEWGEEVSNNAIEVYVHRLRKKIEPGGIRIVTVRGLGYCL
                     ERISGEVPESNVSGVRAG"
     gene            7243..>7398
                     /gene="recA"
                     /locus_tag="AC16HB_18955"
     CDS             7243..>7398
                     /gene="recA"
                     /locus_tag="AC16HB_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646499.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5608925.1"
                     /translation="MDDGRSVAVVDSKERQKALAAALAQIEKQFGKGSIMRLADGEVA
                     EEIDVVST"
BASE COUNT         1184 a         2435 c         2515 g         1264 t
ORIGIN      
        1 gcgcggcgcc gatcgacaac gcaatgcgga gagcaggaga gggcaagcag acgccgacgc
       61 gggaatcggc gcgcagtcta gcggtcgctg tctagcgatg gtttcgcagc atcagcgcgg
      121 cgcgaccatg cgggcgtgca ggtcgttgca ccgagcgcag ccacaaccac agttcctgca
      181 cggccgcgcc aaagaagagc gccaggccgg cgccatccag gttcaggctg tggcagacga
      241 gcgcagccag caggatgagc accgtgggcg catgttcctt caccgagact cgccgcttca
      301 tgatccctgc ctcgcttcga gttcctcgaa ttcgcgcgcg accgtcttcg gcagccgcga
      361 cacctgaaac aggtgcgcct ggcgcgcgtc ggcgcgcagc acctcgaagc gcaggccgtc
      421 gagttcgatc gcatcgccgc gctttggtac gcggccgagc tgatcggtga ccaggccgcc
      481 gatcgtgtcg aattcggctt ccggcaactc ggtgccgaac atcgcgttta ccgcggcgat
      541 ctctgccagc gccttgacac ggtagcggcc gtcggattcg gcgacgatcg catcggcttc
      601 ctcgtcgaag tcgtattcgt cctcgatgtc gccgacgatc tgttcgagca cgtcctcgat
      661 cgtgatcagt ccggccaccc caccatattc gtccacgacg atcgccatgt ggttgcggtt
      721 ggcgcggaag tcgcgcagca gcacgttcag aggcttgctc tccgggatga acacggcggg
      781 ccgcagcatc gagcgcacgt cgaagtcctc gtcgtggtag taacgcacca ggtccttggc
      841 gagcagcacg ccgatgatgt ggtcgcggct cccctcgaag accgggaagc gcgaatgcgc
      901 ggtctcgatc acgtgcggaa tccacttctg cggcggctcg tcgatgtcga tcatgtcgat
      961 ctgcgagcgc ggcaccatca ggtcgcgcgc gcgcaattcg gacacctgca gcacgccctc
     1021 gatcatcgac agcgcgtcgg catcgagcag gttgcgctcg tgtgcgtcgt gcagcgcctc
     1081 gagcagttct tcgcggttct cgggttcgcg gaagatgaac gcggtcaggc gttcgagcag
     1141 ggacttgtga ttctggtggg ccgcgattcg acccggcgta ggttcaggca tgagtgtcgg
     1201 gcggcgtgct gacgctgaac cgaacccggc agttgagacg aattcagcat atcggatgcg
     1261 caggaattcg ccatgacacg tgtccgggtg cgacgggggc gatcgatctt gccaccgccc
     1321 atcaaggatt tcggagaaag tgcccgctaa ccttcgtcgt aaggatttgg gatgccgcac
     1381 gcgtcaagca gtgcgatctc gcgccgctcc atccgtctcg catcgacacg ccgctgatgg
     1441 tcatatccct gcgcgtgcag cgttccatgc acgaccaggt gggcgagatg cgagcgcagc
     1501 ggccggcgct gcgcgcgcgc ctcgcgccgg acgaccggca cgcagatgac gatgtcggcg
     1561 tcgatgagcg ggcgctgcgc gtagtcaaac gtcagcacgt tggtcgcgtg gtcctgaccg
     1621 cggaagcggc gattgagctg ccggccctcg cgctcgccga cgaagcgcag cgtcagccgc
     1681 gcgtcgcgtt ccagcgccgt ggcgatccag cgccgcaggg tgcggcgcgc gggcaggtcg
     1741 gcaaaggcct catcgccctg caccgacagc gatagccgat tcattccggg gctacttggc
     1801 ggcgcccgcg cggcggcgac ggattgtcct tctcggcacg ctcgtaggcc tcgacgatgc
     1861 gcgcgaccag cgggtggcgc accacgtcgg ctgcagtgaa gtaggtgaac gcgatgccgc
     1921 gcacgccgcg caggatgtgc tgcgcctcga tcagccccga ggaactgccg cgcggcagat
     1981 cgatctgcgt gacgtctccg gtgacgaccg ccttcgagcc gaagccgatg cgcgtcagga
     2041 acatcctcat ttgctcgggt gtcgtgttct gcgcctcgtc gagaatcacg aatgcgtgat
     2101 tgagcgtcct gccgcgcatg taggcgagcg gtgcgatctc gatcgtctgc ttttccagca
     2161 gccgctgcgt cttttcgaag ccgagcaggt cgaacagcgc gtcgtacagc ggccgcaggt
     2221 acgggtcgac cttctgcgcg aggtcgcccg gcagaaaccc cagccgctcg ccggcctcga
     2281 ccgccggccg ggtaagcacg atgcgcttga ccgcgtcgcg ctcgagcgca tcgacggcgc
     2341 acgcgaccgc cagataggtc ttgccggtgc cggccgggcc gatcgagaag ctgatgtcgt
     2401 gcgcgagtat cgcctcgatg taatcgcgct gccgcggcgt gcgaccgtgc aggtcgcgcc
     2461 ggcgcgtatg aagctgcggc tgcggttcgc cttcaggctc gaccagcggc tcgcgcgtgc
     2521 ggccgccgcg catctcgacg aaatagagct ggatgtcgtc gatgctgatg ggccgctgcg
     2581 cgcggctcac gaagtggccg agcacgctga cagcctgctg cgcgggcgcc ggtgcgccct
     2641 cgattcggaa ctggtggccg cgccgacgaa tcgcgacgtc gagctcggtc tcgatctggc
     2701 gcaggttgct gtcgagcgcg ccggtcaggt tcgacagctg cgagttgtcg atgtcggccg
     2761 cccatgcgag aaccttcttc gcggtggcgc gcttggccac gtcagacggc gagcggcgcg
     2821 tcacgcacaa cctgctcgcc gcgcagcgag tgcggcagcg cggcggtgat gcgcacgtcg
     2881 accatccgac cggacagttc gatcgcgcgg cctccggcgt cgaagttcac gatccggttg
     2941 ttctcggtgc gccccatcag ttcgtccggg tccttcttcg acgggccctc gaccaggatg
     3001 cgctgccgcg tgccgaccat cacggcgctg atgcgctgct gctgctcgct gagcaacgcc
     3061 tgcagccgct gcagccgctc gagcttcact tcctgcggag tggcgtcggg cagttccgcc
     3121 gccggcgtgc ccgggcgccg gctgtagacg aagctgaagc tcgcgtcgaa gccgatctcg
     3181 gaaacgagct tgagcgtgcg ctcgaagtcg tgctcggttt cgccgggaaa gccgacgatg
     3241 aaatcggacg aaatcgagat gccgggccgc accgcgcgca ggcggcgaat gatcgacttg
     3301 tactcgagca ccgtatagcc gcgcttcatc gcggccaaca tgcggtccga tccagcctga
     3361 acgggcaggt gcaggtggcc gaccagcttc ggcagccgtg cgtacgcgtc gatcagccgt
     3421 ggcgtgaact ccttcgggtg cgaagtcgtg tagcgaatgc gctcgatgcc ggggacttcg
     3481 tgcacgacgt cgaggaggag tgcaaggtcc gcggtctgcc cgtcgcgcat gcgtccgagg
     3541 tatgcattca cgttctggcc gagcagcgtg atttccttca ccccgtgatc ggcgagatcc
     3601 gcgacttccg tcagcacgtc gtcgaacggt cgcgacacct cttcgccacg cgtgtatggc
     3661 accacgcaga aactgcagta cttgctgcag ccctccatga tcgacacgaa cgcgctcgca
     3721 ccttccacgc gcggcggcgg caggtggtcg aacttctcca cctcgggaaa agagatgtcg
     3781 acctgcggag tgccggtcac gcggcggcgc tcgatcaatt gcggcaaccg gtgcagcgtc
     3841 tgcggcccga acaccacgtc cacatacggt gcgcgcgcga cgatcgctgc accttcctgg
     3901 cttgccacgc agccgcccac gccgatgacc aggttcggat tgcgtcgctt caggtgcttg
     3961 acgcggccga ggtcggagaa caccttctcc tgcgcctttt cgcgcaccga gcaggtgttg
     4021 aacaggatga tgtcggcgtc gtccggacgg tcggtgggct gcaggccgga cgcctcgttg
     4081 agcacgtcgg ccatcttggc cgagtcgtac tcgttcatct ggcagccgaa ggtgcggatg
     4141 tagagcttct tcgtcatcgg tatcgcgcgg ccggtttaga tccggtggcg agcggatgaa
     4201 agtctagcgg cgcttggcct cttcgggcgt cagcatccag atcgtgcgca cctgtccctg
     4261 gccgtcgagc tggtagcgaa tcacggtgtc ggcagcgacc tggttcggcg tcaccgtcat
     4321 gttgttcgcg ttcagaattc gcgcgccggg cgacatgcga taggtcttgc cgtcgatcct
     4381 caccgaaccg agctcgatcg ccgtcaactt gccgcgcttg gcgtcggccg ggagcgtgcg
     4441 cagcatgacc tgcccggacg cggcgacgct gaagcacagg gcaatgacgg caacggtcca
     4501 gcgtggcatc gcgtgcttgt agtctggtgg cggatcaggg actcgaaccc cggacacaag
     4561 gattatgatt cctctgctct aaccagctga gctaatccgc cgtgtctgaa acggtctcgc
     4621 ccaggcagcg gcgaggcgcg gattattctt cattcactgc ggccgggtca aatcatcgtc
     4681 aaagccgcac cgtcgacggc gcgccggcga tgtgacggaa ctcaacgctc atccgtgtgc
     4741 caggcccatc ggccgcgcgg ctgttcggat tgctgcccac gtgcagcaat gcgtcgtgtt
     4801 gctcgacgat ctcgcgcacg atcgccagcc ccagcccact gccgtcgacc tgcgtgccga
     4861 gcacgcgata gaagcgttcg aatatcagcg cgcggtcggc ctccggaata ccgggtccgg
     4921 tgtcctcgac ctccaggcgc accgcggccg gaagcgcttc caccctcacg gtgacggtcc
     4981 cgcccccggg tgtgaaacgc agcgcgttgt cgacgaggtt cttcagcatc tcgcgcagcg
     5041 cgaccggcat gccgatgatg cgcgtcgcgg tcgtcgcagt ttccggtccc tcgaagccga
     5101 gatcgatcga acgcgcaagc gcgagcggca cgaattcctg caccagtttg cgcgccagcg
     5161 tggcgaggtc gacgatctcg aacgcaccga gatcggccgc ctgatgctcg gtgcgcgcca
     5221 gcgcgagcaa ctggtcgatc aggtgcgcgg cgcgctcgct cgattcagcg atctgacgca
     5281 ggctgcggcg caactgctgc gggtcggttt cacgcagcgc cagctcggcc tgcgtgcgca
     5341 gtccggcgag cggcgtcttc atctggtgcg cggcgtcggc gagaaagcgc gtctgcgtct
     5401 gcatcgatcg cttcagccgc tccagcagct cgttgaagga gttcacgagt ggccccagct
     5461 cctccggcgc ggccttgggg tcgatcggcg acaggtcgtc cgggcgtcgc gcgcggatgc
     5521 gcccctgcag cgaattcaac ggcgccaggc cgcgcgtcag cgcgaaccac accagcgtca
     5581 ctgcgatcgg cagcacgatg aactgcggga tgatcacgcc gcggatgatg tcgttggcga
     5641 gctgcgagcg gtcggcgagc gtctcggcga cctgcaccag cgcgggctgg ccggtcgcgc
     5701 gcggaaacgc gacccacgtg taggccacgc gcacttcggc gccgcgcatc tggtcgaagc
     5761 gcaacctgat gagtcccggc gcgggcgtct cgtcctcggg cggcagcggc acctcgcgct
     5821 cgccggcgac gaattcgccg cgcagcccga gcaccatgta gttgacgctc tcgccggagt
     5881 cacgccgcaa cacgtcgtgc gccggaatcg gcagctgcag cgacacctgt ccacggtgct
     5941 ccttgacgta gtccgacagc agttcgacgt tctcggccag cgtgcggtcg tacggcgcgt
     6001 cggcgatcga ctgcgcaacc agataagtga tcgtcacgct catcggccag aggagcagca
     6061 gcggcgccag catccaatcg agaatctcgc cgaacagcga ggtctgttcg ttgctcaggc
     6121 gcagcggatt gacgaacagt tggggtggag ccgcgggcgg cgacgcgggc acggcttcac
     6181 cgcgccgatg gccgcgcagc acgaacgacg cgatcgcccg cagccccgca cggcgtgcgg
     6241 tcggaaccgt attgccgtgg ccggcactgg cacgtcgatc gtcgtcgggc atggttgcga
     6301 ctgtagcgcg ggtgcccgcc gcgcgcggac gacgcgtgcg ctcgatcagc cggcgcggac
     6361 gccgctgacg ttcgactcgg gaacttcgcc gctgatgcgc tcgaggcagt agcccagtcc
     6421 gcgcaccgtg acgatccgga tcccgccggg ctcgatcttc tttctcagcc ggtgcacgta
     6481 gacctcgatg gcgttgttgc tcacctcttc gccccattcg cacaggtggt cgacgaggtg
     6541 ttccttgctg accaggcggc ccggtcgcga cagcaggatc tcgagcagcc ccaactcgcg
     6601 gccggacaat tcgagcggct tgtcgttcag cgaggccacg cggccgacct gatcgagcgt
     6661 cagcgggccg tggcgcagca gtgtctgcgc gccacccatc gcgcggcgca tcagcgcgcg
     6721 cgcccgcgcc tcgagttccg aaagggcaaa cggcttggcc atatagtcgt cggcgccgag
     6781 gtcgagtccc ttgacgcgct gctcgacgct gtcggccgcg gtcaggatca gcacgggcgt
     6841 ggcggtctgt cgcgcgcgca tacggcgcag cacctccagg ccggggagct tgggcaggcc
     6901 gaggtcgaga atcagcaggt cgaactgctg cgcggacagc gcggtgtcag ccgcttcgcc
     6961 gtcgctgacg acgtcgacgg cgtagccacc cgcgcgcagg gagcgcgaca gaccgtcggc
     7021 cagtaccgga tcgtcttccg cgatcagtat ccgcacttgt tatgctcgtg ccgcccgagt
     7081 gggcagtcct gtctcccctg tgcggcgagt ctagtgccgg cgcctccgcg gtcgcaaccg
     7141 atcgcaggac ggcttgactc ggctactgaa tgaatctaca gtgatactgt aaatggattc
     7201 agtagtgggc tgagtgtttg accggcgacg acggaggcga cgatggatga cggaaggagc
     7261 gtggcggtgg tggacagcaa ggagcggcag aaggccctgg cggccgcgct cgcgcagatc
     7321 gagaagcagt tcggcaaggg ctcgatcatg cggctcgccg atggcgaggt ggccgaggaa
     7381 atcgatgtcg tctcgacc
//
LOCUS       DBNHBY010000813         6717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1078665, whole genome shotgun sequence.
ACCESSION   DBNHBY010000813 DBNHBY010000000
VERSION     DBNHBY010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1078665"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1072
                     /gene="waaC"
                     /locus_tag="AC16HB_18960"
     CDS             83..1072
                     /gene="waaC"
                     /locus_tag="AC16HB_18960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR5608926.1"
                     /translation="MSAPRILIVKTSSMGDVVHALPLASDLARARPDAAIDWVVEEGF
                     ADIPRLHTAIDRVIPVAVRRWRRAPFARATRTEVRVARRALRDHSYTAVIDCQGLIKS
                     ALIAVSARGPVWGPDRASAREPLAALLYRHPVAVDRAQHAIARNRALGAAVLGAATDD
                     APRFALHVPPLTQPALVDFAERGGYAVLLTNASRITKLWLPERWRALEAWLASRGLAS
                     ALPWGSREEEHDTRARAAGMQCGWVAPSASLLQLAALLRGARVVVGLDTGLTHLAAAV
                     GVPTVGLFCDYDPALVGITGAAPCASLGNVGRPPNADDVIVAVERLLAAADDR"
     gene            1146..2438
                     /gene="waaA"
                     /locus_tag="AC16HB_18965"
     CDS             1146..2438
                     /gene="waaA"
                     /locus_tag="AC16HB_18965"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HIR5608927.1"
                     /translation="MNPHIARLLYPLAWWCTLPLAAGYLVWRALRQREYLRHWGERFL
                     GRGARPAGAPVIWVHAVSVGETRAAQPLIELLGRTHPHVHFTLTHMTPTGRVVGASIA
                     RTLSGRVAQRYLPYDVPCAVHRFLRETKPAVGVLMETEAWPNLLHAARAAGIPVVLVN
                     ARLSGKSAAKAQRYAALIRPAVANLARVAAQSEADRARLATVYDGPIDVVGNMKFDLA
                     PLPQLVEQGQLARSHWGQRPVWLAASTRDGEEALILDALARRQQHVGRLLIVPRHPQR
                     FDDVARLIAERGFAVVRRSSAAWNAPLADDAVLLGDSMGEMSMYYAAADVALIGGSLL
                     PYGAQNLIEACAVGTPVVLGPSTYNFAQAAADALAVGAALQVENVDAALQALEVLTRD
                     PARLQQMRAAALSFAAAHRGATARTAALIGELLPERDA"
     gene            complement(2446..3783)
                     /locus_tag="AC16HB_18970"
     CDS             complement(2446..3783)
                     /locus_tag="AC16HB_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR5608928.1"
                     /translation="MFANRKTAVAVAGALLALAGGAWAQAPARNDLLAIYRDALQNDP
                     VFASARLARQAAEEREPQARSAVLPQINAGLGASATNLDSRTPDFNRTFTSWGPSLSL
                     AVPMYRPQNWEAISQAKLAVVSAEATLAQARQDLMLRVTQAYFDVLAAQDALAAILEN
                     KKGVSEQLAQAKREFEVGTKTIVDTHEAQARFDLTVAQEQTARGDLIVKQNALRAIIG
                     RDPGTLVTLRDAPQLNAPLPKDVEAWAKQAEESSYGVAVAKAATEIAQLETKRQREGT
                     YPTVDLTASASLNRSDGSSTSASTNTARTGTIGLQLNYPLYTGGLIQSRIREAIANEE
                     RARQDLESARRNAAQAARQAYTGVDYGLTQVRALESAETSANSQLESTKLGYRVGVRI
                     NLDVLNAQTQLFNTQRDLKKARYDFLVNGLRLQSAAGALNEKDLESVNALLAR"
     gene            complement(3815..4138)
                     /locus_tag="AC16HB_18975"
     CDS             complement(3815..4138)
                     /locus_tag="AC16HB_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5608929.1"
                     /translation="MKAIHAAELAQWLADPARAAPQLVDVREPWETQICRLPGSELIP
                     MRTVPEALDRLDTERPVICICHHGHRSAHVAMFLLRQGFTDVYNLVGGVDAWARQVDT
                     SMPVY"
     gene            complement(4138..4827)
                     /locus_tag="AC16HB_18980"
     CDS             complement(4138..4827)
                     /locus_tag="AC16HB_18980"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR5608930.1"
                     /translation="MNASTVALDIEKARYNMVEQQVRTWEVLDQTVLDLLLAVKREDF
                     VPPQYRALAFTDMDIPLKVDGYDSGEFMFAPKVEARFLQELGIKPFEHVLEIGTGSGY
                     MAALAAHKAQHVLTVEIDDKLAAFGAANLARAGVRNVKVDVGDAARGWAARAPYDVII
                     VSGSLPLIPDALLAQLKIGGRLSAVVGDAPVMSAQIVKRIADGAYDTLKLFETCVKPL
                     RNAWRPSAFRF"
     gene            complement(4936..5619)
                     /locus_tag="AC16HB_18985"
     CDS             complement(4936..5619)
                     /locus_tag="AC16HB_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5608931.1"
                     /translation="MRAHRAEATAARPRWARRKEARPAELLAAALDLFVERGFAGTRL
                     DDVAARAGVSKGTLYLYFESKEELFKAVVRENLVARISAARDEVSSYAGDTAELLRTL
                     IRRWWHEFGATKAGGISKLMLAESGNFPDITRFFLEEVIEPWHRLVGAVIERGVRRGE
                     FRRVDVETFVQVVTAPMVMLSLWTRSFGPCSARPLDVESFVDTLLDMALVSLQAKEAA
                     PARRRSVPR"
     gene            5795..6622
                     /gene="thiD"
                     /locus_tag="AC16HB_18990"
     CDS             5795..6622
                     /gene="thiD"
                     /locus_tag="AC16HB_18990"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR5608932.1"
                     /translation="MSGPMRVCNVVTIAGVDPSGGAGVLADVKAISAQRAYACAVITA
                     LTAQNTQAVAGIHAVPPTFVREQIDVLFADVAIDAAKIGMLGERAVIEAVALGLQRWA
                     PRYIVLDPVMVAKSGDSLLATDAVGALRDALLPLATVITPNLPEAGVLLEGRAPDSLR
                     EMRQVAERLRDRMNHSGQRWVLLKGGHLPYGDAIDLLTDGDKMIELASPRVATKNTHG
                     TGCTLSSALAALLPQSADVPEAARRAKAYLTKAIENAGLLNVEKGHGPVHHFHAMWK"
BASE COUNT          928 a         2338 c         2379 g         1072 t
ORIGIN      
        1 cagtccgact tccgccgcgc gctgctcgcg gtcaagcccg acgcgcagtt gccgttctag
       61 gcacgaggtt cggcgcgccg cgatgtccgc tccgcgcatc ctgatcgtga agacctcgtc
      121 gatgggcgac gtcgtacatg cgctgcccct ggcgtccgac ctcgcgcgcg cgcggcccga
      181 tgcggcgatc gactgggtcg tcgaggaagg tttcgccgac atcccgcggc tgcatacggc
      241 gatcgaccgc gtgattccgg tcgcggtgcg acgctggcgc cgtgcgccgt ttgcacgtgc
      301 gacccgcacc gaggtgcgcg tcgcacgtcg cgcactgcgc gatcacagct acacggcggt
      361 gatcgattgc caggggctga tcaagagcgc gctgatcgcg gtcagcgcgc gcggtccggt
      421 ctggggcccc gatcgcgcga gcgcgcgcga gcctctggcc gcgctcctgt atcggcatcc
      481 ggtggcggtc gatcgcgcgc aacacgcgat cgcgcgcaac cgtgcgctcg gcgccgcagt
      541 gctcggcgcg gcgaccgacg atgcgccgcg cttcgcgctg cacgtgccgc cgctgacgca
      601 gccggcgctg gtcgacttcg ccgagcgcgg cgggtatgcg gtgctgctga ccaatgccag
      661 ccgcatcacc aagctgtggc tgcccgagcg ctggcgtgcg ctggaggcat ggctggcctc
      721 gcgcgggctg gccagcgccc tgccgtgggg aagccgcgaa gaggagcacg acacccgggc
      781 gcgcgccgcc ggaatgcagt gcggttgggt cgcaccgtcg gcctcgctgc tgcaactcgc
      841 cgcgctgctg cgcggtgcgc gcgtcgtggt cggtctcgac accgggctga cgcacctggc
      901 ggccgccgtc ggtgtgccga cggtgggcct gttctgcgac tacgacccgg cgctggtcgg
      961 catcaccggc gctgcgccgt gcgcgagcct cggtaacgtc ggccgcccgc cgaatgccga
     1021 cgacgtgatc gtggcggtcg agcgcctgct cgccgcggcg gacgatcgat gagcgctgcc
     1081 gggccgtgcc gaacgcacct gcatccccgc gggcgcgccg gcgcgcagcg ctgtcaggac
     1141 cgaacatgaa cccacacatc gcacgcctgc tgtacccgct ggcgtggtgg tgcacgctgc
     1201 cgctcgcggc ggggtacctg gtgtggcgcg cgttgcgcca gcgcgagtac ctgcgccact
     1261 ggggtgagcg ctttctcggt cgcggggcgc gacccgcggg cgcgccggtg atctgggttc
     1321 acgcggtgtc ggtcggcgag acgcgtgccg cgcaaccgct gatcgaactg ctgggccgga
     1381 cccatccgca cgtgcatttc acgctcacgc acatgacgcc gaccggccgc gtggtcggcg
     1441 cgtcgatcgc gcgcacgctg tcgggccgcg tcgcgcagcg ctacctgccg tacgacgtgc
     1501 cgtgcgcggt gcaccgcttc ctgcgcgaaa cgaaaccggc ggtcggcgtg ctgatggaaa
     1561 ccgaggcctg gccgaacctg ctgcacgccg cgcgcgccgc cggcattccg gtcgtgctgg
     1621 tcaacgcgcg gctgtccggg aaatcggcgg ccaaggcgca gcgctacgcc gcgctgatcc
     1681 ggcccgcggt cgcgaatctc gcacgcgtgg cggcccagtc cgaagccgac cgcgcgcgtc
     1741 tggcgaccgt ctacgacggt ccgatcgacg tcgtcggcaa catgaagttc gatctggcgc
     1801 cgctgccgca actcgtcgag caggggcagc tggcgcgatc gcactggggc caacgcccgg
     1861 tgtggctcgc cgccagcacg cgcgacggcg aagaggcgct gatcctcgac gcgctcgcgc
     1921 ggcggcagca acacgtcggg cgcctgctga tcgtgccgcg ccacccgcag cgcttcgatg
     1981 acgtggcgcg gttgatcgca gagcgcggct tcgcggtcgt gcggcgcagc tcggcggcgt
     2041 ggaacgcgcc tctcgccgac gacgcggtgc tgctcggcga ctcgatgggc gagatgtcga
     2101 tgtactacgc ggcggcggac gtcgccctga tcggcggcag cctgcttccc tacggtgcgc
     2161 agaacctgat cgaggcgtgc gcggtcggca cgccggtcgt gctcggcccg agcacgtaca
     2221 acttcgcgca ggcggccgcc gacgcgcttg cggttggcgc ggcattgcag gtcgagaacg
     2281 tcgacgcggc gctgcaggcg ctggaggtgc tcacgcgcga tccggcgcgg ctgcagcaga
     2341 tgcgcgccgc cgcgctgagc ttcgccgccg cgcatcgcgg cgcgaccgcg cgcacggcgg
     2401 cgctgattgg cgagctgctg cctgagcgag acgcgtagtg cgccgtcagc gcgccagcag
     2461 cgcgttcacg ctttcgagat ccttctcgtt cagcgcgccg gccgcggact gcaggcgcag
     2521 gccgttgacg aggaagtcgt agcgcgcctt cttcagatcc cgctgcgtgt tgaagagctg
     2581 cgtctgggcg ttcagcacat cgaggttgat gcggacgccg acgcggtagc cgagcttggt
     2641 cgattcgagc tggctgttgg ccgaagtctc cgccgattcg agcgcgcgga cctgcgtcag
     2701 cccgtagtcg acgccggtgt aagcctggcg cgcggcctgt gcggcgttgc gccgtgcgct
     2761 ctcgagatcc tgcctggcac gctcctcgtt ggcgatcgcc tcgcggatgc ggctctggat
     2821 caggccgccg gtgtaaaggg ggtagttcag ctgcaacccg attgtgccgg tgcgcgccgt
     2881 atttgtgctg gcactcgtgg agctgccgtc ggagcggttc aggctcgccg acgcggtcag
     2941 gtccacggtc gggtaggtgc cctcgcgctg gcgcttggtt tcgagctgcg cgatttcggt
     3001 cgcagccttg gcgaccgcca ccccgtagct gctctcttcg gcctgcttcg cccacgcctc
     3061 gacgtccttg ggcagcggtg cgttgagctg cggcgcgtcg cgcagggtca ccagcgtgcc
     3121 gggatcgcgg ccgatgatcg cccgcaacgc gttctgcttg acgatcaggt cgccgcgcgc
     3181 ggtctgctcc tgtgcgacgg tcaggtcgaa gcgcgcctgt gcctcgtgcg tgtcgacgat
     3241 ggtcttggtg ccgacctcga actcacgctt ggcctgcgcc agctgctccg agaccccttt
     3301 cttgttttcc aggatcgcgg ccagcgcgtc ctgcgcggcg agcacgtcga agtacgcctg
     3361 cgtgacgcgc agcatcaggt cctggcgcgc ctgtgccagc gtggcctcgg cgctgacgac
     3421 ggcgagcttc gcctgcgaga tcgcctccca gttctgcggc cggtacatcg gcacggcgag
     3481 agacaacgac ggcccccagc tcgtgaaggt gcgattgaag tcgggcgtgc gcgaatcgag
     3541 gttggtcgcc gatgcgccga gcccagcgtt gatctgcggc agcacggccg agcgcgcctg
     3601 cggctcgcgc tcctcggcgg cctggcgcgc gagtcgcgcc gatgcgaaca ccgggtcgtt
     3661 ctgcagcgcg tcgcggtaga tcgccagcag gtcgttgcgt gccggcgcct gcgcccacgc
     3721 gccccccgcc agtgccagca acgcgccggc aaccgccact gctgttttcc ggttcgcgaa
     3781 catcgttgtg ctcctccgag atcttcggtt cagttcaata gaccggcatg gacgtgtcga
     3841 cctgccgcgc ccaagcgtcg acgccgccca ccaggttgta gacatcggtg aagccctgcc
     3901 gcagcaggaa catcgccacg tgcgcactgc ggtggccgtg atggcagatg cagatgacgg
     3961 gtcgttcggt gtccagccgg tccagcgctt cgggcaccgt gcgcatcggg atcagctcgg
     4021 aacccgggag gcggcagatc tgcgtctccc acggttcgcg cacgtcgacg agctgcggcg
     4081 ctgcgcgcgc cggatcggcc agccactgcg cgagttcggc cgcatggatc gccttcatca
     4141 aaaccggaaa gccgatggcc gccaggcgtt gcgcagcggc ttcacgcagg tttcgaagag
     4201 cttgagcgtg tcgtacgcgc cgtctgcgat gcgcttcacg atctgcgcgc tcatcaccgg
     4261 cgcgtcgccg acgaccgcgg acagccggcc gccgatcttc agctgcgcga gcagcgcgtc
     4321 ggggatgagc ggcagcgacc ccgacacgat gatcacgtcg tagggcgcgc gcgccgccca
     4381 gccgcgcgcc gcgtcgccga cgtcgacctt gacgttgcgc acgccggcgc gcgcgagatt
     4441 cgcggcgccg aacgcggcga gcttgtcgtc gatctcgacg gtcagcacgt gctgcgcctt
     4501 gtgcgcggcg agcgccgcca tgtaacccga gccggtgccg atctccagca cgtgctcgaa
     4561 cggcttgatg ccgagttcct gcaggaagcg cgcctcgacc ttgggcgcga acatgaactc
     4621 gccgctgtcg tagccgtcga ccttcagcgg gatgtccatg tcggtgaacg cgagcgcgcg
     4681 gtactgcggc ggcacgaaat cctcgcgctt gaccgccagc agcaggtcga gcacggtctg
     4741 gtcgagcacc tcccacgtgc gtacctgctg ctcgaccatg ttgtagcggg ccttctcgat
     4801 gtcgagcgca accgtcgaag cgttcattgc tgccacattc attcaagtgc cgggacgaca
     4861 ggcgcggatc ttagcaatgg tgtccgcgcg ccccgttcga ttcatgccct gatcccgtcg
     4921 ttccgtcgca tgcgttcaac gcgggacgga tcgccgccgt gccggcgccg cctcctttgc
     4981 ctgcagcgag acgagcgcca tgtcgagcaa cgtatcgacg aagctctcca catcgagcgg
     5041 ccgcgccgag cacggtccga acgagcgcgt ccacaggctc agcatcacca tcggcgcggt
     5101 gacgacctgc acgaacgtct cgacgtcgac gcgccggaac tcgccgcgcc ggacgccgcg
     5161 ctcgatcacc gcgccgacca gccggtgcca gggctcgatc acctcttcga ggaaaaagcg
     5221 cgtgatgtcg gggaaattcc cgctctcggc cagcatcagc ttgctgatgc cgccggcctt
     5281 cgtcgcgccg aactcgtgcc accagcgccg gatcagcgtg cgcagcagct ccgccgtgtc
     5341 gcctgcgtac gatgacacct cgtcgcgcgc ggcgctgatg cgcgcgacga ggttctcgcg
     5401 cacgaccgcc ttgaagagtt cctccttgct ctcgaagtac aggtatagcg ttcccttgga
     5461 cacgccggcg cgcgcggcca cgtcgtcgag ccgcgtgccg gcgaagccgc gctcgacgaa
     5521 gaggtccagc gcggcggcca gcagctcggc cggccgcgcc tccttgcggc gcgcccagcg
     5581 cggccgcgcc gcggtcgctt cggcgcggtg cgcgcgcatc gggcgggcga tctcattaat
     5641 gactagctag tcatcaattt agatcgatcg cgcggcgggg tcaatgaagc gcgccgcgcc
     5701 gcggcagccc gtatccttgg cgcgaaccgg cagagcagtg cgaggttaca gacatggcga
     5761 ggagatccga cggcgatctc ggcagggcag ggggatgagc ggaccgatgc gcgtgtgcaa
     5821 cgtggtcacg atcgccggcg tcgatccctc cggcggtgcc ggagtgctcg cggatgtgaa
     5881 ggcaatcagc gcgcagcgtg cctacgcctg cgccgtgatc accgcgttga cggcgcagaa
     5941 cacgcaggcg gtggccggca tccacgccgt gccgcccacg ttcgtgcgcg agcagatcga
     6001 cgtgttgttt gccgacgtgg cgatcgacgc ggccaagatc ggcatgctcg gcgagcgcgc
     6061 ggtgatcgag gcggtcgcgc tcgggctgca gcgctgggcg ccgcgctaca tcgtgctcga
     6121 cccggtgatg gtcgccaaga gcggcgattc actgcttgcc acggacgcgg tcggcgcgct
     6181 gcgcgacgcg ctgctgccgc tggcgaccgt gatcacgccc aacctgcccg aggccggcgt
     6241 gctgctcgag ggtcgcgcgc ccgacagcct gcgcgagatg cggcaggttg ccgagcggct
     6301 gcgcgaccgg atgaatcact ccggtcaacg ctgggtgctg ctgaagggcg ggcacctgcc
     6361 gtatggcgac gcgatcgatc tgctgaccga cggcgacaag atgatcgaac tcgcgtcacc
     6421 gcgcgtggcg acgaagaaca cgcacggcac cgggtgcacc ctgtcgtccg cgctggcggc
     6481 gttactcccg cagtcggccg acgtgcccga ggcagcgcgg cgcgcgaagg catatctgac
     6541 gaaagcgatc gagaacgccg gtctactgaa tgtcgagaaa gggcacgggc cggtgcatca
     6601 cttccatgcg atgtggaagt aagaggttga tgcgctctcc gccgcgcggt ctcccgcgcg
     6661 attgagcgcg acaagctact tgttgatcgc caccttcggc accgcgcctt cctgcgg
//
LOCUS       DBNHBY010000814         1769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1284151, whole genome shotgun sequence.
ACCESSION   DBNHBY010000814 DBNHBY010000000
VERSION     DBNHBY010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1284151"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..>1769)
                     /locus_tag="AC16HB_18995"
     CDS             complement(118..>1769)
                     /locus_tag="AC16HB_18995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5608933.1"
                     /translation="GPGAIVLAPSHPDNTVGGDRLRYLAWDVGPRVSKNDNDFYRALV
                     GLRGTVAGWDFDTALLHSETSLTNTRTGFLRYSAVRAALGDPTSPLFPWRIGDNANLN
                     SASLYAAISPTIHADATSKMDIVDVKGSRELMQLPGGPLALAVGAEYRHESTKLTPQT
                     FTDQGDIIGLGFSAYEGSRSVYAVYAEVVAPIVKTLEASAAVRYDHYPSDNSTTPKVG
                     LKWTPNKMIALRGTYAEGFRAPNPAENGKGGLAAFTTARDPVRCPGGTPLPGADAADC
                     GQQIAIITTPNPDLKPEKSKNYTLGIVFEPFSTTSIAVDWWQIKRTNEINQTTVAAAV
                     AGAGTVLRSDNNLPGIPNSGTLLAVSAPYINSASTKVEGVDLDLKQRFNLANYGTLQA
                     GVVWTHMSTFKRTETDGTSIEWVGTHGNCDVTNCIGTPSDRVNFSLTWDRGPWQVATN
                     VNYISSIKNRDEDGGDCNNTFANGQDAPNGCRIASFTTVDLSARWSPTKQLDVFGSIQ
                     NLFDRVAPLDPHTYGAVNFNPLHVSGAIGRFYTIGLKYKFF"
BASE COUNT          297 a          542 c          582 g          348 t
ORIGIN      
        1 acgcgcgtcg aaaccgccac gcgatccgga aacttgtctc tcgaggtctg gtgcaatgcg
       61 aaaaaaaggg ctcggggtcc gaagaccccg agcccccgag aagctgctca ggcagggtta
      121 gaagaacttg tacttcagcc cgatcgtgta gaagcggcca atcgcgccgc tgacgtgcag
      181 cggattgaaa ttgacggcgc cataggtgtg cggatccagc ggagccacgc ggtcgaaaag
      241 gttctggatc gacccgaata cgtcgagttg cttggtcggc gaccaacggg ccgacaggtc
      301 gacggtcgtg aacgacgcga tcctgcaacc gttaggtgcg tcttggccat tggcaaacgt
      361 attgttgcag tcacccccgt cttcgtcccg gttcttgatg ctgctgatgt agttcacgtt
      421 cgtggccact tgccacgggc cccggtccca ggtcagcgag aaattcaccc gatcgctcgg
      481 cgtgccgatg cagttggtca catcgcagtt gccgtgcgta ccgacccact cgatcgaggt
      541 gccatccgtc tcggtccgtt tgaatgtcga catgtgggtc cagaccacgc ccgcctgcag
      601 ggttccatag ttggcgaggt tgaagcgctg cttcagatcg agatcgacac cctcgacctt
      661 ggtgctggcc gagttgatgt acggcgcgct caccgccagc aacgtgcccg agttcgggat
      721 gccaggcaga ttgttgtcgg aacgcagtac cgtgcctgcg ccggcgaccg ccgccgcgac
      781 tgtcgtctgg ttgatctcgt tcgtgcgctt gatctgccac cagtcgactg cgatgctggt
      841 cgtgctgaac ggctcgaaca cgatgcccag cgtgtagttc ttggatttct ccggcttgag
      901 atcgggattc ggcgtcgtga tgatcgcgat ctgctggccg caatccgcgg cgtccgcacc
      961 gggaagcggc gtgccgccgg ggcaacgcac ggggtcgcgc gccgtggtga acgccgccag
     1021 acctcccttg ccgttctcgg ctgggttcgg cgcgcggaag ccctccgcgt aggtgccgcg
     1081 cagcgcaatc atcttgttcg gcgtccactt caggccgacc ttcggcgtcg tcgaattgtc
     1141 actggggtaa tggtcgtagc gcaccgcggc ggatgcttcg agcgtcttga cgataggcgc
     1201 gaccacttcg gcgtagacag cgtacacact gcgcgaaccc tcgtacgcgg agaagccgag
     1261 gccgatgatg tcgccctggt cggtaaacgt ctgcggcgtc agcttcgtgg actcgtggcg
     1321 gtattcggcg cccaccgcaa gggcgagcgg tccgcccggc agttgcatca gttcgcgcga
     1381 gcccttgacg tcgacgatat ccatcttgct ggtggcgtcc gcgtggatcg tcggcgaaat
     1441 cgcggcatac aggctcgcgc tgttcagatt ggcgttgtcg ccgatgcgcc agggaaagag
     1501 aggactggtt ggatccccga gcgcagccct caccgcgctg tagcggagaa agccggtgcg
     1561 cgtgttggta aggctggttt ccgagtgcag cagcgcagtg tcgaaatccc atccggctac
     1621 tgtgccgcgc aagccgacga gtgcgcggta gaaatcgttg tcgttcttgc tgacacgcgg
     1681 gcccacgtcc caggccagat accgcaggcg atcgccgccc accgtgttgt cgggatggct
     1741 cggtgccagg acgattgcgc ccgggccgc
//
LOCUS       DBNHBY010000815         3631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1620311, whole genome shotgun sequence.
ACCESSION   DBNHBY010000815 DBNHBY010000000
VERSION     DBNHBY010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1620311"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..457)
                     /locus_tag="AC16HB_19000"
     CDS             complement(101..457)
                     /locus_tag="AC16HB_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5608934.1"
                     /translation="MKYLCLVYLDERRLPELPDEDCVAYDTAIRASGHCIASEALESV
                     QTATTVRVRSGKLSVTDGPFAETKEQLAGFYMIEAKDLDEAIRLAAGIPPARVGSIEL
                     RPIRPIREAVAQARGR"
     gene            complement(454..960)
                     /locus_tag="AC16HB_19005"
     CDS             complement(454..960)
                     /locus_tag="AC16HB_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5608935.1"
                     /translation="MKLTHRITPCLWFDDQAEDAARLYTAIFPNSRITAISRYGEAGR
                     EIHGRPPGSVMVASFELDGQPFTALNGGPLFKFSEAISLQVNCDTQEEVDHYWDRLSA
                     GGDEKAQQCGWLKDRFGLSWQIIPTVLPKYMTDPDPRRSNRVMQALLKMKKLDIAQLQ
                     RAYEGAAG"
     gene            complement(957..2270)
                     /locus_tag="AC16HB_19010"
     CDS             complement(957..2270)
                     /locus_tag="AC16HB_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5608936.1"
                     /translation="MAADAVPDARAAVDAIYRSESRRVFATLVRLLGDFDLAEEALAE
                     AFRAALETWPRDGLPGNPRAWLVSAGRFKAIDAIRRQRRFDSLEDHAEFAESIADETP
                     ASGAGEEIADDRLRLVFTCCHPALAPDAQVALTLREVCGLTTEAIAHAFLTPAPTLAQ
                     RIVRAKAKIRDARIPYQVPAPDELPQRLDSVLRVIYLVFNEGYAASSGDALIRRDLSA
                     EAIRLGRLLVELLPEPEAIGLLALMLLTEARRAARTNADGDLVLLADQDRSLWDGAQI
                     AEGVALVERALAPGRIGPYALQAAIAAVHAEAPSAPATDWAQIVGLYDVLARIEPSPV
                     VELNRAVAVAMRDGPAAGLARVDAILARGELADYHFAHSARADFCRRLGRTVDAQAAY
                     ERALALARTEPERRFLSRRLAALRDVDSRPSRSTTDRTATSGDRR"
     gene            complement(2270..2965)
                     /locus_tag="AC16HB_19015"
     CDS             complement(2270..2965)
                     /locus_tag="AC16HB_19015"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR5608937.1"
                     /translation="MPQHIGIVACSAEGAALCYRTICVEGAQLLGRHAHPEVSLHTHS
                     LAAYVDRLDRGDLQGVAELMLDSARKLAQIGADFLICPDNTLHQAFDRVAANSPRPWL
                     HIAGVVADEAAARGMRRLGLTGTRWLVASDVYPRALTARGLHCVLPSATERDEIDRII
                     MDELVCGQFRPESVACLQRAIARMQREGCDAVILGCTEIPLVISDANSPLPTLDSTRL
                     LARAALRRAVRGN"
     gene            3246..>3631
                     /locus_tag="AC16HB_19020"
     CDS             3246..>3631
                     /locus_tag="AC16HB_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5608938.1"
                     /translation="MNEPIVNIADIELQPRPAAFTPIGAAAERFEARLAPIGARLGAR
                     KLGYNVTAVAPGKRAFPFHNHRVNEEMFFVLQGTGEIRIGGNTYSIRAGDIIACPPGG
                     KETAHQIVNTGAEELRYLAVSTKLSP"
BASE COUNT          585 a         1296 c         1198 g          552 t
ORIGIN      
        1 gtagaagcgc atcgcctcag cgcagttgcc gttgaaggcg aggtatgcaa tcggttgtgg
       61 catgacggtc ctttcgttgc tggcggttgg gccgacccaa tcatcggcca cgagcctgcg
      121 ccaccgcttc tcgaatcggc cggatcgggc gcagctcgat cgacccgacg cgcgccggcg
      181 ggatgccggc ggcaaggcgg atcgcttcgt ccaggtcctt cgcctcgatc atgtagaagc
      241 cggcgagctg ctccttcgtc tcggcgaacg gcccgtcggt aaccgacaac ttcccgctgc
      301 gcacgcgcac cgtcgtggcg gtctgcaccg attcgagcgc ttccgatgcg atgcaatggc
      361 cgctggcgcg gattgcggtg tcgtaggcga cacagtcctc gtcgggcagc tccggcagac
      421 gtctttcgtc gaggtagaca aggcagaggt acttcatccg gcggctcctt cgtacgcacg
      481 ctgcagctgc gcgatgtcga gcttcttcat cttcagcagc gcctgcatca cgcggttcga
      541 ccgtcgcgga tcgggatcgg tcatgtattt cggaagcacc gttggaatga tctgccatga
      601 caggccgaac cggtccttca gccagccgca ctgctgggcc ttctcgtcgc cacccgcgga
      661 cagccggtcc cagtagtgat cgacttcttc ctgcgtgtcg caattgacct gcagcgaaat
      721 cgcctcgctg aacttgaaca acggaccacc gttcaacgcc gtgaacggtt gcccatcgag
      781 ctcgaacgac gcgaccatca ccgaccccgg cggccggccg tggatctccc gccccgcttc
      841 gccatagcgg gagattgcgg tgatcctcga attgggaaag atcgcggtgt agaggcgcgc
      901 cgcgtcctcg gcctgatcgt cgaaccacag gcacggcgtg atccgatgcg tgagcttcat
      961 cgtcggtctc ctgaagtggc agtgcgatcg gtagtcgaac ggcttggcct cgaatcgaca
     1021 tcccgcagcg cggccagccg tcggctcagg aagcggcgct cgggctcggt cctggccagc
     1081 gccagcgcgc gctcgtaggc agcctgcgcg tcgaccgtcc gaccgagccg gcggcagaaa
     1141 tcggcgcgcg ccgaatgcgc gaagtgatag tcggccagtt cgccgcgcgc caggatcgcg
     1201 tcgacccgcg cgagacctgc cgccggaccg tcgcgcatcg ccaccgcgac cgcgcggttc
     1261 agttcgacga ccggcgacgg ctcgatgcgc gcgagcacgt cgtacagccc cacgatctgc
     1321 gcccagtcgg tcgccggtgc gctcggcgcc tccgcgtgca ccgccgcgat cgccgcctgc
     1381 agcgcgtacg ggccgatgcg gcccggcgcc agcgcgcgct cgaccagtgc cacgccttcg
     1441 gcaatctgcg cgccatccca cagcgaacgg tcctggtcgg cgagcaatac gaggtcgcca
     1501 tccgcattcg tgcgcgcggc gcgccgtgcc tcggtgagca gcatcagcgc cagcaggccg
     1561 atcgcctccg gctcgggcag cagttcgacc agcagccggc cgaggcggat cgcctcggct
     1621 gacaagtcgc ggcgaatcag cgcgtcgccg ctcgacgccg cgtagccctc attgaacacg
     1681 aggtagatca cgcgcagaac gctgtcgagc cgttgcggca attcgtccgg cgccggcacc
     1741 tggtacggga tgcgcgcgtc gcggatcttc gctttcgcgc gcacgatgcg ctgtgcgagc
     1801 gtcggcgccg gcgtcaggaa cgcgtgcgcg atggcctcgg tcgtcagccc gcacacctcg
     1861 cgcagcgtca gcgcgacctg cgcgtcgggc gcaagcgccg gatgacagca ggtgaagacc
     1921 agccgcaggc ggtcgtcggc gatttcctcg ccggcgccgc ttgctggcgt ctcgtccgcg
     1981 atcgattcgg cgaactcggc gtgatcttcg agcgaatcga agcgccgctg ccggcggatc
     2041 gcatcgatcg ccttgaagcg gcccgcggac accagccacg cccgcggatt gcccggcaag
     2101 ccgtcgcgcg gccacgtttc gagcgcagcg cgaaacgctt ccgccagcgc ctcttccgcg
     2161 agatcgaagt cgcccagcaa gcgcaccagc gtggcgaaca cgcggcgcga ttcgctgcga
     2221 tagatcgcat cgactgcggc gcgcgcgtcc ggcaccgcgt ccgcggccat cagtttcccc
     2281 gcaccgcgcg gcgcagcgcg gcgcgcgcca gcaatcgcgt ggagtcgagc gtcggcagcg
     2341 gcgagttggc gtccgagatg accagcggga tctcggtgca gccgaggatc accgcatcgc
     2401 agccctcgcg ctgcatgcgc gctatcgccc gctgcaggca cgcgaccgac tccggccgga
     2461 actgcccgca caccaactcg tccatgatga tgcggtcgat ctcgtcgcgt tccgtggccg
     2521 acggcagcac gcagtgcagg ccgcgcgcgg tcaaggcgcg cggatacacg tcgctcgcga
     2581 ccagccagcg cgtgccggtc agcccgagcc ggcgcatgcc gcgcgcggct gcttcatccg
     2641 cgaccacccc ggcgatgtgc agccacggcc gcggcgagtt cgcggcgacc cgatcgaacg
     2701 cctggtggag cgtgttgtcg gggcagatca ggaagtcggc tccgatctgc gccagctttc
     2761 gcgcggagtc gagcatcagc tcggctacgc cctgcaggtc gccgcgatcg agccgatcga
     2821 catacgcagc aagggaatgc gtatgcagcg aaacctccgg atgcgcgtgc cgcccgagca
     2881 gttgcgcgcc ctcgacgcag atcgtccggt agcacagcgc cgcgccctcc gccgagcagg
     2941 cgacgatacc gatgtgctgc ggcatgccga tcgattccgt tccagtgcgc gtgccgcgcg
     3001 aaccttacgc cgaaccgcgc cccgccgcga tcccggttcg ttacacctgt aatggttcgg
     3061 cgtacgcgcc gaaacgaccg tgcaatgcgg acgtggcacg ttcgatcggt ccgccatgaa
     3121 gcagcgctcg attgcgcgca ggtccgcata gggcgttacc ggcacgcgcg cgtcgctggc
     3181 cgcggacatc cgcgcggcaa tccaaatcgc aggagcgcgg catcaaccca tcgacccgag
     3241 ccgccatgaa cgaacccatc gtcaacatcg ccgacatcga actgcaaccg cgccccgcgg
     3301 cctttacgcc aattggcgcg gccgcggaac gcttcgaggc gaggctcgcg ccgatcggcg
     3361 cccggctcgg ggcgcggaag ctcggctaca acgtgaccgc ggtggcgccg ggcaagcgcg
     3421 cgtttccatt ccacaaccat cgcgtgaacg aagagatgtt cttcgtgctg cagggcaccg
     3481 gcgaaatccg catcggcggc aatacgtatt cgatccgcgc cggcgacatc atcgcctgcc
     3541 cgcccggcgg caaggagacc gcgcaccaga tcgtcaacac cggcgccgag gaactgcgct
     3601 acctcgcggt gagcacgaag ctgtccccgg a
//
LOCUS       DBNHBY010000816         3596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1891814, whole genome shotgun sequence.
ACCESSION   DBNHBY010000816 DBNHBY010000000
VERSION     DBNHBY010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1891814"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1335)
                     /gene="asd"
                     /locus_tag="AC16HB_19025"
     CDS             complement(208..1335)
                     /gene="asd"
                     /locus_tag="AC16HB_19025"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012338485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR5608939.1"
                     /translation="MKVGIVGWRGMVGSVLMDRMAAENDFDLFDSVFFSTSNAGGAAP
                     KVKRAEAALKNANDLAALKACDTIITCQGGDYTNDTHPKLRAAGWNGYWIDAASALRM
                     KDDAVIILDPVNLPVIKNALAKGVKDYIGGNCTVSLMMLAIDGLLKADLVEWVSAMTY
                     QAASGAGAPNMRELIEQMGCAHAAVRDLLSDPRSAILEIDRKVTAALRADDLPRANFG
                     VALAGSLIPWIDKDLGNGVSREEWKGGAECNKILGRPPMGQPGSVVVEGICVRIGAMR
                     CHSQALTIKLKRDVPLAEIERTLAAANRWVRVIPNKRDDSIRELSPARVSGSMEIPVG
                     RLRKLAMGGEYLSAFTIGDQLLWGAAEPLRRMLRLLLNRLD"
     gene            complement(1362..2429)
                     /gene="leuB"
                     /locus_tag="AC16HB_19030"
     CDS             complement(1362..2429)
                     /gene="leuB"
                     /locus_tag="AC16HB_19030"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR5608940.1"
                     /translation="MKIAVLPGDGIGTEIAAEAVKVLNALGERLELEFADVGGAAYEK
                     HGHPLPAATLKLAQQADAVLFGAVGGWKYDKLERALRPEQAILGLRKGLGLFANFRPA
                     ILYPELAAASSLKPEVVSGLDILIVRELTGDVYFGQPRGRRAAPDGPFAGQPEAFDTM
                     RYSKPEIERIAHVAFLAARRRKKKVTSVDKSNVLETFQFWKDVVTEVHAQYRDVELEH
                     MYVDNAAMQLVRAPKNFDVIVTGNMFGDILSDEAAMLTGSIGMLPSASLNERNQGLYE
                     PSHGSAPDIAGKGVANPLATILSAAMLLRYSGNRAEQADRIEAAVRRVLAGGLRTPDV
                     WSAGTRKVGTKEMGDAVVAAL"
     gene            complement(2492..3136)
                     /gene="leuD"
                     /locus_tag="AC16HB_19035"
     CDS             complement(2492..3136)
                     /gene="leuD"
                     /locus_tag="AC16HB_19035"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR5608941.1"
                     /translation="MERFSVHRGLVVPLDRANVDTDAIIPKQFLKSIHRSGFGPNLFD
                     EWRYRDHGEPGKDNAKRPLNPDFVLNQPRYKGASVLLARKNFGCGSSREHAPWALAQY
                     GFRALIAPSFADIFHNNCLKNGLLPIVLPELVVDRLFHEVAGFVGYQLTIDLPRQVVV
                     EPGGGEHAFEITPFRKQCLINGLDDIGLTLRHRDEILAFEKQRLARFPWLVKTT"
     gene            complement(3140..3265)
                     /locus_tag="AC16HB_19040"
     CDS             complement(3140..3265)
                     /locus_tag="AC16HB_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR5608942.1"
                     /translation="MKRVLLVLAVLFAVAACNTFEGMGKDIQKAGETIEGAAKKK"
     gene            complement(3285..>3596)
                     /locus_tag="AC16HB_19045"
     CDS             complement(3285..>3596)
                     /locus_tag="AC16HB_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5608943.1"
                     /translation="KRLSPNVKLAMVVPGSGLVKAQAEREGLDRVFTAAGFEWREPGC
                     SMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRRL
                     G"
BASE COUNT          588 a         1266 c         1071 g          671 t
ORIGIN      
        1 cgcaaacgca aggctgaccg ctaatgcgag cgcggaccgc gccgatcttg aactcgaatt
       61 gttcgttgcc acggctgcct ctcaagggcg gtcaagtttg atttcgaact taacatcagt
      121 gacttagcgg tgcaagctga caaaccgccg caactgctcg tcctgatggc tgatagacgg
      181 gaatctatcc acggctccgc ttcaggctca gtcgagccgg ttcaggagca gtcgcagcat
      241 gcgacggagc ggttccgccg caccccacag cagctggtca ccgatcgtga atgcgctcag
      301 gtactcgccg cccatcgcca gcttgcgcag acggccgacc ggaatctcca tgctgccgct
      361 gacgcgggcg ggtgagagct cgcgaatgct gtcgtcgcgc ttgttcggaa tcacgcgcac
      421 ccagcggttt gccgctgcca gcgttctctc gatctccgcc agcggcacat cacgcttcag
      481 cttgatcgtc agtgcctggc tgtggcagcg cattgcaccg atgcgcacgc agatcccttc
      541 gaccaccacc gagcccggct gacccatcgg cggccggccg agaatcttgt tgcattccgc
      601 gccgcccttc cactcctcgc ggctgacccc gttgcccaga tccttgtcga tccacggaat
      661 gaggctgccc gccagcgcca cgccgaaatt ggccctgggc aagtcgtccg cgcgcagcgc
      721 cgcggtgacc ttgcggtcga tttcaaggat cgccgatctc gggtccgaca gcagatccct
      781 gaccgccgcg tgggcgcatc ccatctgctc gatcagttcg cgcatgttgg gcgccccggc
      841 gccggacgcg gcctgatagg tcatcgcgct gacccactcg accaggtcgg ccttcaaaag
      901 cccgtcgatc gcgagcatca tcaggctcac ggtgcagttg ccgccgatgt agtccttgac
      961 accttttgcc agcgcgttct tgatcaccgg caggttgacc gggtcgagaa tgatcaccgc
     1021 gtcgtccttc atccgcagcg cggacgcggc gtcgatccag tagccgttcc agccggccgc
     1081 gcgcagcttc ggatgggtgt cgttcgtgta gtcgccgccc tggcaggtga tgatcgtgtc
     1141 gcatgccttc agcgcagcga gatcgttcgc attcttcaat gccgcctcgg cgcgcttgac
     1201 cttaggcgcc gcgccacccg cgttcgacgt gctgaagaac accgagtcga acagatcgaa
     1261 gtcattttcc gcggccatcc ggtccatcag taccgatccg accatgccgc gccacccaac
     1321 gattccgact ttcatcactg cacctctttc gtctgcatcc gtcacagcgc cgcgaccacg
     1381 gcgtcaccca tttccttcgt accgaccttg cgcgtgcctg cgctccagac atcgggcgtg
     1441 cgcaagccgc ctgcaagcac gcgcctgacc gcggcctcga tgcgatccgc ctgctccgcg
     1501 cggttgcccg aataacgcag cagcatcgcc gccgacaaga tcgtggcgag cggattggcc
     1561 acgcccttgc ccgcgatgtc cggtgcgctg ccatggctcg gctcgtacaa cccctggttg
     1621 cgctcgttca gcgacgccga cggcagcatg ccgatcgagc cggtcagcat cgccgcctcg
     1681 tcgctgagga tgtcgccgaa catgttgccg gtcacgatca cgtcgaagtt cttcggcgca
     1741 cgcacgagct gcatggccgc attgtcgacg tacatgtgtt ccagttccac gtcgcggtac
     1801 tgcgcgtgca cctcggtcac cacgtccttc cagaactgga acgtttccag cacgttggac
     1861 ttgtcgacgc tggtcacctt cttcttccgc ctgcgcgcag cgagaaacgc gacgtgcgcg
     1921 atacgctcga tctccggctt cgaatagcgc atcgtgtcga acgcctccgg ctgccccgcg
     1981 aacggaccat ccggtgcggc gcggcgccca cgcggctggc cgaagtagac gtcgccggtc
     2041 agctcgcgca cgatcaggat atcgagaccg gagacgactt ccggtttcag cgacgacgcc
     2101 gccgccagtt cgggatacag gatcgcgggg cgaaagttcg cgaacaaccc cagacccttg
     2161 cgtaacccga ggatcgcctg ctcgggacgc agcgcgcgct cgagcttgtc gtacttccag
     2221 ccgccgaccg cgccgaagag cactgcgtcg gcctgctgcg ccagcttgag cgttgccgcc
     2281 ggcagcggat ggccgtgctt ttcgtatgca gcgccgccca cgtcggcgaa ctccagctcc
     2341 aaccgttcgc cgagcgcgtt cagcaccttg accgcctcgg cggcgatctc ggttccgatg
     2401 ccgtcgccgg gcagcactgc gatcttcatg gttgcttcct cgcggactcc atttcactcc
     2461 gcatgcgccc gcgacgttgc gaagtgcgaa ttcaggtcgt cttcaccagc cacggaaacc
     2521 gggccagccg ctgtttctcg aaggccagga tctcgtccct gtgccgcagt gtcaggccga
     2581 tgtcatcgag tccgttgatc aggcactgct tgcggaacgg cgtgatttcg aacgcgtgtt
     2641 cgccgccgcc gggttcgacg accacctgcc gcggcaggtc gatcgtcaac tggtagccga
     2701 cgaagcccgc gacctcgtgg aagagtcgat cgaccacgag ttccggcagc acgatcggca
     2761 gcaggccgtt cttgaggcag ttgttgtgga agatgtcggc aaagctcggc gcaatgagcg
     2821 cgcggaaacc gtactgcgcc agcgcccatg gtgcatgctc acgcgacgag ccgcagccga
     2881 agttcttgcg cgcgagcagg accgacgcgc ccttgtagcg tggctggttc agcacgaagt
     2941 ccggattcag cgggcgcttc gcgttgtcct tgcccggctc gccgtgatcc cggtagcgcc
     3001 attcgtcgaa caggttcggg ccgaagcccg accgatggat cgacttcagg aactgcttcg
     3061 ggatgatcgc gtcggtgtcg acattggcac ggtcgagcgg cacaacgaga ccccggtgga
     3121 cggagaaacg ttccattgcc tacttcttct tcgccgcgcc ttcgatcgtc tcgccggcct
     3181 tctggatgtc cttgcccatg ccttcgaacg tgttgcaggc cgcgacggcg aacaggacgg
     3241 ccagcaccag cagcactctc ttcatcgaat ctttcccttt ctcgctatcc cagtcgccgg
     3301 acgtcgacga aatgcccttc gagcgccgcc gccgccgcca tcgccggcga caccaggtgc
     3361 gtgcggccgc ccgcgccttg ccggccctcg aagttgcggt tcgatgtcga cgcgcagcgc
     3421 tcacccggat ccagccggtc ggcgttcatc gccagacaca tcgaacagcc cggctcgcgc
     3481 cactcgaatc cggccgcggt gaacacgcgg tcgagccctt cgcgctcggc ctgcgccttc
     3541 accagccccg agcccgggac caccatcgcc agtttcacgt tcggcgacag gcgctt
//
LOCUS       DBNHBY010000817         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1620374, whole genome shotgun sequence.
ACCESSION   DBNHBY010000817 DBNHBY010000000
VERSION     DBNHBY010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1620374"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..2044
                     /gene="msbA"
                     /locus_tag="AC16HB_19050"
     CDS             266..2044
                     /gene="msbA"
                     /locus_tag="AC16HB_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR5608944.1"
                     /translation="MIDPSLQRPAFYADPAFRRMLRLVLVHKAWLAVAVVAMLLTAAT
                     EASIPKLTGMLLDQGFYQRNDWAGIWIPAAFVGLFVIRGLATFASSYLLNHVSQIVLL
                     DLRAVMFERLLHWPQTTLENTPSGVVISKFINEATNALNLTAEVMTTAVRDSLTVLAL
                     LAVLVYYNWQLTLVILVVAPLVALVLRAFSARLRRLNLQNQQMLGEMTRAVQEAHEGS
                     RVIKVYGGFDYEGERFGAINNKLRRFAMKMQIAWSAASPLTQIIAAIGLALVIGTALW
                     QARAAHFSAGDFMTFLTASLLLLQPLRHLAGLNGPLARMLAAAESVFAQVDSAQEADS
                     GTRTLERAAGAIELHAVTHQYPGTAQPALVDFSLAVRPGEMVALVGASGAGKTTVINL
                     IPRFIDPTSGRIRIDGIDTRELTLASLRRQVALVSQDVVLFDDTIAANIAYGAARRAS
                     LAQIRAAAEAAYLLPLIEALPHGLETRVGEGAVKLSGGQRQRLSIARALLKDAPILLL
                     DEATSALDSESERFIQASLERLLRGRTTIVVAHRLSTIERADRIAVLDAGRLVEIGTH
                     AQLLARGGLYSNLYRIQFAAPMEVTS"
     gene            2041..>2416
                     /locus_tag="AC16HB_19055"
     CDS             2041..>2416
                     /locus_tag="AC16HB_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226784.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HIR5608945.1"
                     /translation="MTANPQRISRYPVPDLKDLPDDIRTRILAVQEKSGFVPNVFLTL
                     AHRPDEFRAFFAYHDALMEKNSGVTKAEREMIVVATSGANQCHYCVVAHGAILRIRAK
                     NPLVADQVAINYRKADITPRQTA"
BASE COUNT          379 a          818 c          817 g          402 t
ORIGIN      
        1 gcggcgaggg gaccattgcg tgtgcaagga cccgagacgc aacaacgttg tgcgcccaat
       61 gcgaccccgc cccgaagggt tgcgacgata agccggcgtc tgtctcgttg gcagacttgg
      121 ccgtaggctc tgctactgtc ctgcgcctgc cgcctagcat ccgccggctt ctcgtcgtaa
      181 cgcgatccat cgtggattct gagataggtt ctaacgtcgc cggacgacgc ggcgagccgc
      241 gcgattctag ggccgtgaca tcgcgatgat cgatccctcc ctgcaacgcc ccgcgttcta
      301 tgccgacccc gccttccgtc gcatgctgcg gctggtgctg gtgcacaagg cgtggctggc
      361 agtcgcggtc gtggcgatgc tgctcaccgc ggcgacggag gcgtcgatcc cgaagctgac
      421 cggcatgctg ctcgaccagg gcttctacca gcgcaacgac tgggcgggca tctggattcc
      481 ggcggccttc gtcggtctgt tcgtcatccg gggtctcgcg actttcgcca gcagctacct
      541 gctgaaccac gtgtcgcaga tcgtgctgct ggacctgcgc gcggtgatgt tcgagcggct
      601 gctgcactgg ccgcagacga cgcttgagaa cacgccgtcg ggcgtggtca tctccaagtt
      661 catcaacgag gcgacgaacg cgctgaacct gaccgccgag gtgatgacca ccgcggtgcg
      721 cgattcgctg accgtgctcg cgctgctggc ggtgctggtc tactacaact ggcaactcac
      781 gctcgtgatc ctggtcgtcg cgccactggt ggcgttggtg ctgcgcgcgt tcagcgcgcg
      841 cttgcggcgc ctgaacctgc agaaccagca gatgctgggc gagatgaccc gtgcagtgca
      901 ggaggcgcac gagggcagcc gcgtgatcaa ggtctacggc ggcttcgact acgagggcga
      961 gcgcttcggt gcgatcaaca acaagctgcg ccgcttcgcg atgaagatgc agatcgcgtg
     1021 gtcggcagcc tcgccgctga cgcagatcat cgccgcgatc gggctcgccc tcgtgatcgg
     1081 caccgcgctg tggcaggcgc gcgctgcgca tttctccgcc ggcgacttca tgactttcct
     1141 gaccgcgtca ctgctgctgc tgcagccgct gcggcacctg gcgggactga acggcccgct
     1201 ggcgcgcatg ctggcggcgg cggagagcgt gttcgcgcag gtcgactcgg cgcaggaggc
     1261 cgacagcggc acgcgcacgc tcgagcgcgc ggcgggcgcg atcgaactgc acgcggtcac
     1321 gcaccagtat ccgggcaccg cgcagccggc gctggtcgac ttttcgctcg cggtgcggcc
     1381 gggtgagatg gtcgctctgg tcggcgccag cggcgccggc aagaccacgg tgatcaacct
     1441 gatcccgcgc ttcatcgatc ccacgtccgg gcgaatccgg atcgacggca tcgacacgcg
     1501 cgagctgacg ctcgcgagcc tgcggcggca ggtcgcgctg gtatcgcagg acgtcgtgct
     1561 gttcgacgac acgatcgccg ccaatatcgc ctacggcgcc gcgcgcaggg cgtcgcttgc
     1621 gcagatccgc gccgccgcgg aagcggcgta cctgctgccg ctgatcgaag cgctgccgca
     1681 tggactcgaa acccgcgtcg gcgagggagc ggtcaagctg tccggcggcc agcgccagcg
     1741 gctgtcgatc gcgcgcgcgc tgctgaagga cgcgccgatc ctgctgctcg acgaggcgac
     1801 gtcggcgctc gactcggaat cggagcgctt catccaggcc tcgctcgagc ggctgctgcg
     1861 cgggcgcacg acgatcgtgg tcgcgcaccg gctgtcgacg atcgagcggg ccgaccgcat
     1921 cgccgtgctc gacgccgggc ggctggtcga gatcggcacg cacgcgcagt tgctggcgcg
     1981 tggcggcctg tattcgaatc tgtaccgtat ccagtttgca gctcccatgg aggttacgtc
     2041 atgacggcaa acccacaacg aatcagccgc tatccggttc cggacctgaa ggatctgccg
     2101 gacgacatcc gcacccgcat cctcgccgtg caggagaagt cgggtttcgt gcccaacgtg
     2161 ttcctgacac tggcacaccg gcccgacgag ttccgcgcct tcttcgccta ccacgatgcg
     2221 ctgatggaga agaacagcgg cgtcaccaag gccgagcgcg agatgatcgt ggtcgcgacc
     2281 agcggcgcca accagtgcca ctactgcgtg gtcgcgcacg gcgcgatcct gcgcatccgc
     2341 gccaagaacc cgctcgtcgc ggatcaggtt gcgatcaact accgcaaggc cgacatcacg
     2401 ccgcggcaga cggcga
//
LOCUS       DBNHBY010000818         2547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1012638, whole genome shotgun sequence.
ACCESSION   DBNHBY010000818 DBNHBY010000000
VERSION     DBNHBY010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1012638"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="ubiG"
                     /locus_tag="AC16HB_19060"
     CDS             <1..507
                     /gene="ubiG"
                     /locus_tag="AC16HB_19060"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR5608946.1"
                     /translation="SMAARGAQVTGIDLADKPLQVAELHALESGARVGYRKVLAEELA
                     REMPAAFDVVTCMEMLEHVPDPAQTVHACATLVRSGGAVFFSTINRNPKSFLFAIVGA
                     EYVLRLLPAGTHEYAKFIRPSELAQFARAAGLTVGEIVGLTYNPLTKRFALGGDTAVN
                     YLMACRRP"
     gene            504..1166
                     /gene="gph"
                     /locus_tag="AC16HB_19065"
     CDS             504..1166
                     /gene="gph"
                     /locus_tag="AC16HB_19065"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450706.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR5608947.1"
                     /translation="MSRVQLVLFDLDGTLVDTAPDLAAAANRQRAEQGLGPLPIEELR
                     PMASHGARGMIGRALGRAPGDADYEALRLQFLAYYEQALCVHSRLFDGIDQTLGALEG
                     SGVRWGIVTNKLARFTAPLVEQLGLAERAACVVSGDTTPHAKPHPAPLVHAIAQARAT
                     AAASIYVGDDLRDIQAGRAAGVATVIAAYGYLGADADIRRWGGDHIIERPIDLLALIG
                     AD"
     gene            1173..>2547
                     /locus_tag="AC16HB_19070"
     CDS             1173..>2547
                     /locus_tag="AC16HB_19070"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR5608948.1"
                     /translation="MPFSRRARIVALAAVGVLAAAIVGLWLGVPAAARWGIETVGSRE
                     VGRTLRVGEVRFNPFTLTATLTGLSVAGLPEEKAPLLTIDALTANLSAASLRHRAPVI
                     EALQAHGIRANVVRVAENRFNFSDIVDRLLAQPPSPEPARFALYNVEIEDAAIAFDDR
                     VRNTTHALTAIRLGVPFVSSLPDDVQIKVHPSFAAQLDGDPIALTAESQPFDATLDTS
                     VALKLSDIEVPKYLGYVPLRLNFELPRAKLDTDLRIHFRRSTPASKDAPARAAQLSVS
                     GALSLRDFAYAERGGMNLVSWKRLDVTLDEVAVFDAHAKVRSIALEGAQANIVRRADG
                     SIAGLGAMTQPVELSPAPAPEAKGGAGKPFTFEIGAVKVSDAAVDFRDDDVRFTRRVA
                     PVTIQIDGLSNRAGSTAKLSANLAADDKTQVALNGDIALAPLKLALTAELTALPLPSL
                     MPYLRSFT"
BASE COUNT          398 a          867 c          885 g          397 t
ORIGIN      
        1 tcgatggcgg cgcgcggcgc gcaggtgacc ggaatcgatc tggcggacaa gccgctgcag
       61 gtcgccgagc tgcacgcact tgaaagcggc gcgcgcgtcg gctatcgcaa ggttttggcc
      121 gaggagctcg cgcgcgagat gccggccgcg ttcgacgtgg tcacctgcat ggagatgctc
      181 gaacacgtgc ccgacccggc gcagaccgtg cacgcgtgcg ccacactcgt gcggtcgggc
      241 ggcgccgtgt tcttttccac catcaaccgc aatccgaaat cattcctgtt cgcgatcgtg
      301 ggcgccgagt acgtgctgcg gctcctgccc gcgggcacgc acgagtatgc gaagttcatc
      361 cgccccagcg agctcgcgca gttcgcgcgc gcggcggggc tcaccgtggg cgaaatcgtc
      421 gggctgacct acaacccact gacgaagcgg ttcgcgctcg gcggcgacac cgccgtcaac
      481 tacctgatgg cgtgccggcg accgtgagcc gcgtgcagct ggtgctgttc gatctcgacg
      541 gcacgctggt ggacaccgcc cccgacctcg cagccgcggc caatcggcaa cgcgcggaac
      601 aggggctggg tccgctgccg atcgaggaac tgcgaccgat ggcgagccac ggggcgcgcg
      661 gcatgatcgg ccgcgcactc ggccgcgctc cgggcgatgc ggactacgag gcactgcggc
      721 tgcagttcct tgcctactac gagcaggcgc tgtgcgtgca ttcgcgcctc ttcgacggaa
      781 ttgatcaaac gctgggggcc ctggaaggtt ccggcgtacg ctggggcatc gtgacgaaca
      841 agctcgcccg cttcaccgcg ccgctggtcg agcagctcgg cctggcggag cgcgccgcgt
      901 gcgtggtcag cggcgacacg acgccgcacg cgaagccgca cccggcgccg ctggtgcacg
      961 cgatcgcgca ggcgcgcgcg acggccgcgg cgtcgatcta tgtcggcgac gacctgcgcg
     1021 acatccaggc cggtcgcgcc gcaggtgtgg cgacggtgat cgccgcctac ggctacctgg
     1081 gtgcggatgc cgacatccgc aggtggggcg gcgatcacat catcgagcgc ccaatcgacc
     1141 tgctggcgct gatcggagcg gattgatccg cgatgccgtt ttcgcgccga gcccggatcg
     1201 ttgcgctggc cgctgtcggc gtgctggccg cggcgatcgt cggcctgtgg ctcggtgtgc
     1261 cggcggctgc gcgctggggc atcgagaccg tgggctcacg cgaggtcggt cgcactctgc
     1321 gcgtgggcga ggtgcgattc aatccgttca cgctgaccgc gacgctcacc gggctcagcg
     1381 tcgccggatt gccggaagag aaggcgccgc tgctgacgat cgatgcactt acggcgaacc
     1441 tgtccgcggc gtcactgcgg catcgtgcgc cggtgatcga agcgctgcag gcgcacggca
     1501 tccgcgccaa cgtcgtgcgc gtggccgaga atcgtttcaa cttcagcgac atcgttgatc
     1561 gcctgctggc gcagccgcca agtcccgaac cggcgcgttt cgcgctctac aacgtcgaga
     1621 tcgaagacgc tgcgatcgcc tttgacgacc gcgtgcggaa cacgacgcat gcgctgacgg
     1681 cgatccggct cggcgtgccg ttcgtgtcca gcctgcccga cgacgtgcag atcaaggtgc
     1741 atccgtcgtt cgcggcgcag ctggacggcg atccgatcgc gctgaccgcg gagtcgcagc
     1801 cgttcgacgc cacgctggac accagcgtgg cgctgaaact gtcggacatc gaggtgccga
     1861 agtacctcgg ctacgtgccg ctgcgactga acttcgagct gccgcgcgcc aagctcgaca
     1921 ccgacttgcg cattcatttc cggcgcagca cgccggccag caaggatgcg ccggcacgcg
     1981 ctgcgcagct ttcggtcagc ggggcgctgt cattgcgcga ctttgcgtac gccgaacgcg
     2041 gcggcatgaa cctcgtctcc tggaagcgtc tcgacgtgac gctcgacgaa gtcgccgtgt
     2101 tcgacgctca tgccaaggtg cggtcaatcg cgctcgaagg cgcgcaggcg aacatcgtgc
     2161 ggcgcgcaga cggatcgatc gcgggccttg gggcaatgac gcagccggtc gaactgtctc
     2221 ccgcgccggc gccggaagcg aaaggaggtg ccggcaagcc cttcacgttc gagatcggcg
     2281 cggtgaaggt gagcgacgcc gccgtcgact tccgcgacga cgacgtgcga ttcacgcgcc
     2341 gcgtcgcgcc agtcacgatc cagatcgacg gactgtcgaa tcgggccggc agcacggcca
     2401 agctgtccgc gaacctggca gccgacgaca agacccaggt cgcactgaac ggcgacattg
     2461 cactggcgcc gctgaagctc gcgctgacgg cggagctgac cgcgcttccg ctgccgagcc
     2521 tgatgccata cctgcgttcg ttcacca
//
LOCUS       DBNHBY010000819         1980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1079113, whole genome shotgun sequence.
ACCESSION   DBNHBY010000819 DBNHBY010000000
VERSION     DBNHBY010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1079113"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..>1980)
                     /locus_tag="AC16HB_19075"
     CDS             complement(2..>1980)
                     /locus_tag="AC16HB_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR5608949.1"
                     /translation="GGSAGYDHKGMGITARGAWESVKRHFRELGVDTQSSDFTVAGVG
                     DMSGDVFGNGMLLSRHIRLLATFDHRHIFLDPSPDPSRSFEERKRLFELPRSSWADYN
                     AELISAGGGVWPRSAKSVAISPQVRKSLGIDAETLTPNELVTAILKAPVDLLYNGGIG
                     TYIKAESESHADVGDRANDAVRINGSELRCKVVAEGGNLGVTQRGRIEAALAGVRINT
                     DAIDNSAGVDTSDHEVNIKILLGLAIADGELTEKQRNSLLAEMTDDVAALVLRDNYFQ
                     TQALSIARRIGVRLIDQQARFIRFLEKGGRLNRAIEFLPTDEEIEERKARGQGLTSPE
                     LAVLLAYSKMWLFDELLASDVPEDPWVGTALARYFPRKLVERFEAWIPRHPLRREIVA
                     THVLNSMINRVGSTFVHRMSEMTGAPPPQIVRAYLLTREVFGYVPLWQQIEALDGKVP
                     DAVQIDMIAEASRLLTRAVAWFTRSRRLNEPMEATHKRLAPAVAGLRARLAPGAQASP
                     KASAWIQAGVPENLAASAAAVQGLYAALDVAEIAESTRRPLDEVAEVHASVGAHLGLT
                     KLRAQIDQLPADSYWQSLAKAALGDDLAGLQRAIALDAVNQDGDGAAARLAAWEARNG
                     PALERARRLLAEFGDKGADLAMLSVALRELRNLA"
BASE COUNT          284 a          694 c          664 g          338 t
ORIGIN      
        1 gctaggcgag attgcgcaat tcacgcagcg cgaccgacag catcgccaga tccgccccct
       61 tgtcgccgaa ctccgcgagc aaacgtcgcg cgcgctccag cgccggaccg ttgcgtgctt
      121 cccacgcggc gagccgcgcc gcggcgccgt cgccatcctg attcaccgca tcgagcgcaa
      181 ttgctcgctg caggccggcc aggtcgtcgc ccagcgcggc tttcgcgagg ctctgccagt
      241 agctgtcggc cggcaattgg tcgatctgcg cgcgcagctt ggtcaggccc aggtgcgcgc
      301 cgacgctggc gtgcacctcg gccacttcgt cgagcgggcg ccgcgtgctt tcggcgatct
      361 cggcgacgtc gagcgcggcg tacaggccct gcaccgctgc cgcgctggcg gcgaggttct
      421 ccggcacgcc ggcctggatc catgccgagg ccttcggtga cgcctgcgcg cccggcgcaa
      481 gtcgcgcgcg gagccccgcg accgcggggg cgaggcgctt atgcgtcgct tccatcggtt
      541 cgttcagccg gcgcgagcgc gtgaaccacg cgaccgcgcg cgtcagcaac cggctggcct
      601 cggcgatcat gtcgatctgc accgcgtcgg gcaccttgcc atcgagcgcc tcgatctgct
      661 gccacagtgg cacatagccg aatacctcgc gcgtcagcag gtaggcgcgc acgatctggg
      721 gcggcggcgc gccggtcatc tcgctcatgc ggtgcacgaa ggtcgagccg acgcgattga
      781 tcatgctgtt gagcacgtgc gtggcgacga tctcgcgccg cagcggatgg cgcgggatcc
      841 acgcctcgaa tctctcaacc agcttgcgcg ggaagtaacg cgccagcgcg gtgccgaccc
      901 acgggtcttc cggcacgtcg gacgcgagca gttcgtcgaa cagccacatc ttgctgtagg
      961 caagcagcac cgccagctcg ggactggtca ggccctggcc gcgtgccttg cgttcctcga
     1021 tctcctcgtc ggtcggcagg aactcgatcg cgcggttcag tcgcccgccc ttctcgagga
     1081 agcggatgaa gcgcgcctgc tggtcgatca gccgcacgcc gatgcggcgg gcgatcgaca
     1141 gcgcctgcgt ctggaagtag ttgtcgcgca ggaccagcgc agccacgtcg tcggtcatct
     1201 cggccagcag cgaattgcgc tgcttttccg tcaactcgcc atcggcaatc gcaagcccca
     1261 gcaggatctt gatgttgact tcgtgatcgg acgtgtcgac acccgcggag ttgtcgatcg
     1321 catcggtgtt gatgcgcacg ccggccagcg ccgcctcgat gcggccgcgt tgcgtgacgc
     1381 cgaggttgcc cccctcggcg acgaccttgc agcgcagctc gctgccgttg atgcggaccg
     1441 cgtcgttggc gcggtcaccc acgtcggcgt gcgactcgct ttcggccttg atgtaagtgc
     1501 cgatgccgcc gttgtagagc aggtcgaccg gtgccttcag aatcgccgtc accagctcgt
     1561 tcggcgtcag cgtctcggca tcgatgccga gactcttgcg cacctgcggc gagatcgcaa
     1621 cgctcttggc gcttctcggc cacacgccac cgccggcgga gatcagctcg gcgttgtagt
     1681 cggcccacga cgagcgcggc agctcgaaca ggcgcttgcg ctcttcgaag ctccggctcg
     1741 ggtcggggct cgggtcgagg aagatatggc gatggtcgaa cgtggcgagc agccggatgt
     1801 ggcgcgacag cagcatgccg ttgccgaaca cgtcgcccga catgtcgccg acaccggcga
     1861 ccgtgaagtc gctcgactgc gtgtcgacgc ccagttctcg gaagtggcgc ttgacgctct
     1921 cccacgcacc gcgcgcggtg atgcccatgc ctttgtggtc gtagcccgca ctgccgccgg
//
LOCUS       DBNHBY010000820         3847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1554270, whole genome shotgun sequence.
ACCESSION   DBNHBY010000820 DBNHBY010000000
VERSION     DBNHBY010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1554270"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1989
                     /gene="secD"
                     /locus_tag="AC16HB_19080"
     CDS             115..1989
                     /gene="secD"
                     /locus_tag="AC16HB_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5608950.1"
                     /translation="MNRYPLWKYLVIALALLIGATYTTPNFFGEAPAVQVSSQKVTVK
                     ADEVLLAKVEGLLKAGGVEPEATYLDTVGLNTTIRVRLRDTDTQIKAKDILEAALNPD
                     KQDPTFVVALNLLSRSPRWLASLHALPMYLGLDLRGGVHFLMQVNTKEAVDKRLDSLV
                     GDARTLLREKEVRHAGIVRNNQQIEIRFRDAETRAKAEAVLSDLLRELAFREDSAGGD
                     LLLVGTMTPAAAKTVADNALKQNITTLHNRINELGVAEPVIQQQGADRVVVQLPGVQD
                     TARAKDIIGRTATLESRLVDISAEGQAAVLGNAPVPFGSERFTVGRGAPVVLKKEVIF
                     SGQQLQGAQATFDENQRPSVSIELNEAAGRIMRKVTRENIKKPMAVVLFEKGRGEVLT
                     VATIQSEFGSRFQITGLDSPTVANDLALLLRAGALAAPMEIIEERTIGPSLGADNIRS
                     GFNSTRYGFMAIAIFMIAYYMVVGLISVLSLSVNLLLLVALLSLLQATLTLPGIAAIA
                     LTLGMAIDSNVLINERIREELRKGRPPNQAIAEGYEKAFATILDSNITTLIAGVFLFM
                     LGSGPIRGFAVVHCLGILTSIFSAVFVSRGFVAIAYGHRKRLNAISIGQVWKPEAARG
                     "
     gene            1993..2925
                     /gene="secF"
                     /locus_tag="AC16HB_19085"
     CDS             1993..2925
                     /gene="secF"
                     /locus_tag="AC16HB_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5608951.1"
                     /translation="MEFFRIRRTIPFMRYALIFNAISFVTFLVAVGFLLFRGLNLSVE
                     FTGGTVMEVSYPQAADLEKVRAAIESRAGGEFQVQNFGTSRDVLIRLPLEKGVSSGEQ
                     AKQVMATLKTQDPGVELRRVEFVGPSVGEELAHDGALALLMVCLGIMLYLAFRFEWKF
                     SVAAIIANLHDVILVVGMFAIFRWEFSLPVLAAVLAVLGYSVNESVIIFDRVREHFRT
                     MRRASVDEVIDSAITATISRTIITHGSTLMMTLAMLIFGGPALHYFALALTIGICFGV
                     YSSVFVAAAIARYLGVKREDLVKPQKKEDQQIVP"
     gene            2985..3647
                     /locus_tag="AC16HB_19090"
     CDS             2985..3647
                     /locus_tag="AC16HB_19090"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5608952.1"
                     /translation="MLHRVRVWDLPTRLFHWTLVVLVAFSVVTAKIGGNAIDWHERSG
                     YAILTLLVFRILWGLMGSRYARFASFLVPPFEALRRARDVLSPRVRLPRVGHDPLGGW
                     AVIALLTVLGVQAVTGLFSYDDVATWGPWAKFVSNATSTWLTTLHRYNEKALYALITM
                     HVTAIAYYRFAKRESLVVPMITGDRVGIVAAAAEDDATTRLRAVVLAVLVAALVGYLV
                     SL"
     gene            complement(3687..>3847)
                     /locus_tag="AC16HB_19095"
     CDS             complement(3687..>3847)
                     /locus_tag="AC16HB_19095"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR5608953.1"
                     /translation="NQAKFKDYADKLNTEVGRLLAAARANDLGATRAAFGDVGKACKG
                     CHDDFRKD"
BASE COUNT          600 a         1269 c         1290 g          688 t
ORIGIN      
        1 aagggcacct tgaaagcatt gtgaagcgcg aaatgtgaaa tgtgaatcgt tgtcgcaagc
       61 cgcgcactgc cggatcgttc accattcacg tttcacgcgt ctcgtttcgc cgccatgaat
      121 cgctaccctc tctggaagta cctcgtcatc gcgctcgcgc tgttgatcgg ggcgacctac
      181 acgacgccga acttcttcgg cgaggcgccg gccgtgcagg tgtcgtcgca gaaggtgacg
      241 gtcaaggcgg acgaagtgct gctggccaag gtcgagggac tgctgaaggc gggcggcgtc
      301 gaaccggagg ccacgtacct cgacaccgtc ggcctgaaca ccacgatccg cgtgcggctg
      361 cgtgacaccg acacgcagat caaggccaag gacatcctcg aagcggcgct gaacccggac
      421 aagcaggacc cgacgttcgt ggtcgcgctg aatctgctct cgcgctcgcc gcgctggctg
      481 gcgagcctgc acgcgctgcc gatgtacctc ggcctcgatc tgcgcggcgg cgtgcacttc
      541 ctgatgcagg tgaacaccaa ggaggcggtc gacaagcgac tcgattccct cgtcggcgac
      601 gcgcgcacgc tgctgcgcga gaaggaagtc cggcacgccg gcatcgtgcg caacaaccag
      661 cagatcgaga tccgcttccg cgatgccgag acgcgcgcca aggcggaggc ggtcctgtcc
      721 gacctgctgc gcgagctggc gttccgcgag gacagcgccg gcggcgacct gctgctggtc
      781 ggcacgatga cgccggccgc ggccaagacg gtcgccgaca acgcgctgaa gcagaacatc
      841 accactctgc acaaccggat caacgagctc ggcgtggccg aaccggtgat ccagcagcag
      901 ggtgccgacc gcgtcgtggt gcagctgccc ggcgtgcagg acaccgcgcg cgccaaggac
      961 atcatcggcc gcaccgcgac gctcgagtcg cggctggtcg acatcagcgc cgagggtcag
     1021 gcggccgtgc tcggcaatgc gccggtgccg ttcggctcgg agcgcttcac cgtcggccgc
     1081 ggcgcgccag tcgtactgaa gaaggaagtg atcttctccg gacagcagtt gcagggtgcg
     1141 caggcgacct tcgacgagaa ccagcgcccg tcggtgtcga tcgagctgaa cgaggccgcc
     1201 ggccgcatca tgcgcaaggt cacgcgcgag aacatcaaga agccgatggc ggtcgtgctg
     1261 ttcgagaagg gccgcggcga ggtgctgacg gtcgccacga tccagtccga attcggctcg
     1321 cgcttccaga tcaccgggct cgacagcccg accgtggcca acgacctggc gctgctgctg
     1381 cgcgccggcg cgcttgccgc gccgatggag atcatcgagg aacgcacgat cggcccgagc
     1441 ctcggcgccg acaacatccg ctccggcttc aattcgaccc gctacggctt catggcgatc
     1501 gcgatcttca tgatcgccta ctacatggtg gtcgggctga tctcggtgct ttcgctgtcg
     1561 gtcaacctgc tgctgctggt cgcgctgctg tcgctgctgc aggcgacgct gacgctgccc
     1621 ggaatcgcgg cgatcgcgct cacgctcggc atggcgatcg actccaacgt gctgatcaac
     1681 gagcgcatcc gcgaggaact gcgcaagggc cggccgccga accaggcgat cgccgagggc
     1741 tacgagaagg cgttcgcgac cattctcgac tccaacatca cgacgctgat cgccggcgtg
     1801 ttcctgttca tgctcgggtc cggtccgatc cgcggcttcg cggtcgtgca ctgcctgggc
     1861 atcctcacgt cgatcttctc ggcggtgttc gtgtcgcgcg gcttcgtcgc gatcgcgtac
     1921 ggccaccgca agcggctcaa cgcgatctcg atcgggcagg tctggaagcc ggaagcggcg
     1981 aggggataga cgatggaatt cttccgcatc cgccgcacga ttccgttcat gcgctacgcg
     2041 ttgatcttca acgcgatctc gttcgtcacg tttctcgtcg cggtcggctt cctgctgttc
     2101 cgcgggctga acctgtcggt cgaattcacc ggcggcacgg tgatggaggt gtcgtatccg
     2161 caggccgccg acctggagaa ggtccgcgcg gcgatcgaat cgcgcgccgg cggcgagttc
     2221 caggtgcaga acttcggcac ttcgcgcgac gtgctgattc ggctgccgct cgagaagggc
     2281 gtgagctccg gcgagcaggc caagcaggtg atggccacgc tgaagaccca ggaccccggg
     2341 gtcgagctgc ggcgcgtcga attcgtcggc ccgtcggtgg gcgaggagct cgcgcacgac
     2401 ggcgcgctcg cgctgctgat ggtctgcctc ggcatcatgc tgtacctggc gttccgcttc
     2461 gaatggaagt tctcggtggc ggcgatcatc gccaacctgc acgacgtgat cctggtggtc
     2521 ggcatgttcg cgatcttccg ctgggagttc tcgctgccgg tgctggccgc ggtgctcgcg
     2581 gtgctcggct actcggtcaa cgagtcggtc atcatcttcg accgggtgcg cgagcacttc
     2641 cggacgatgc gcagggcgag cgtggacgag gtgatcgact ctgccatcac ggcgaccatc
     2701 tcgcgcacga tcatcacgca cggctcgacg ctgatgatga cgctggccat gctgatcttc
     2761 ggcggcccgg cactgcacta cttcgcgctc gcgctcacga tcggcatctg cttcggcgtg
     2821 tactcgtcgg tgttcgtcgc cgcggcgatt gcgcgctacc tcggcgtgaa gcgcgaggac
     2881 ctggtcaagc cgcagaagaa ggaagaccag cagatcgttc cgtgaagggt gcggaatagg
     2941 tacgcggcgc ccgctgttat gtcgctgtga ccgcatttct caccatgctc caccgcgtgc
     3001 gcgtctggga cctgccgacc cggctgttcc actggacgct cgtcgtgctc gtcgccttct
     3061 ccgtcgtcac cgccaagatc ggcggcaatg cgatcgactg gcacgagcgc agcggctacg
     3121 cgatcctgac gctgctcgtg ttccgcatcc tgtggggact catgggcagc cgctatgcgc
     3181 gcttcgccag ctttctcgtg cctccgttcg aagcgctgcg tcgcgcgcgc gatgttcttt
     3241 cgccgcgcgt gcggctgccg cgcgtgggac acgatccgct cggcggctgg gccgtgatcg
     3301 cactgctcac ggtgctgggc gtgcaggcgg tgacaggcct gttctcatac gacgacgtcg
     3361 cgacctgggg cccgtgggcg aagttcgtgt cgaatgcgac cagcacctgg ctcacgacgt
     3421 tgcatcgcta caacgagaaa gccctgtacg cgctgatcac gatgcatgtg acggcgatcg
     3481 cgtactaccg cttcgccaag cgcgagagcc tcgtcgtgcc gatgatcacc ggcgaccggg
     3541 tcggcatcgt cgcggcggcg gcggaggacg atgcgacgac gcggttgcgc gcggtcgtgc
     3601 tcgccgtgct cgtcgccgcg ctggtcggtt acctcgtctc gctttgagtg ccgcagtgcg
     3661 ggccgccggg cggcccgcct ctccggctag tccttgcgga aatcgtcgtg gcagcccttg
     3721 caagccttgc cgacgtcgcc gaaggccgcg cgcgtcgcgc cgaggtcgtt cgctctcgcc
     3781 gcggcgagga gacggccaac ttcggtattc agcttgtccg cgtagtcctt gaacttggcc
     3841 tgattgg
//
LOCUS       DBNHBY010000821         2063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_673839, whole genome shotgun sequence.
ACCESSION   DBNHBY010000821 DBNHBY010000000
VERSION     DBNHBY010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_673839"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1642
                     /locus_tag="AC16HB_19100"
     CDS             263..1642
                     /locus_tag="AC16HB_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5608954.1"
                     /translation="MMGQQPGGQKKLFYSFNLDDHIPADHLLRGIDQFLDLGELHQHL
                     APFYSHTGRPSIDPKLLIRMLVVGYCFGIRSERRLCEEVHLNLAYRWFCRLGLEDPVP
                     DHSTFSKNRHGRFRDSDTLRFVFEQVLERCVREGLVGGEGFAVDASLVAADASRQRGV
                     PGGDADWSDRRSISRPVREYLAALDQDEVEKKTPVNISLTDPAARWSAVWGPAFYAYS
                     ANYLIDIAAGIVVDVEATPSHKLDEINATKTMIERVEARFDLKPRRLIGDTAYGTAEM
                     LAWMVDEKQIEPHVSVWDRTQRDDGTLSSSDFRWDPEGDEYHCPQGQVLRRERRAFKT
                     PRSHVTKADTIIYRASQADCTACPLKRRCCPNTPRRKIARSIYESARDVARELAKTDA
                     YKQSRKDRKKVEVLFAHLKRILKLDRLRLRGPSGAHDEFLLAATAQNLRRMAKWLYPA
                     EPETANMAI"
BASE COUNT          432 a          612 c          645 g          374 t
ORIGIN      
        1 actccgatct ttgaaccagg tacgcgccgg agaggcattg ccaggcgccc cgtggcgtgc
       61 ggcggcgcgt cttggactcg gtgatttggc cgccgtacct ggtacctcgg acctcgtgcc
      121 agtgctgagg actcagtact tcgaacgacg aactgcgatt ggtccaaaga ccgagttcga
      181 ccgattgtgt tgaaaaactc ccttgaaaac ggacgcttgg tgactatgct catagcaggg
      241 ccggcatggg ggagaagcga ggatgatggg acaacagcct ggcggacaga agaagctgtt
      301 ctactcattc aacctcgacg atcacattcc tgcggatcac ctgctacggg gaatcgatca
      361 gttcctcgat ctaggtgagt tgcaccagca ccttgccccg ttctacagcc ataccggccg
      421 gccctcgatt gatccaaagc tgctgattcg catgctggtc gtgggttact gcttcggaat
      481 ccgatccgag cggcgactct gcgaggaagt ccatctgaat ctggcgtatc ggtggttctg
      541 tcggctggga ttggaagacc cggtgccgga ccactcgacg ttctccaaga accggcatgg
      601 ccgtttccgt gacagcgaca cgctgcggtt cgtgttcgag caggtcttgg agcgctgtgt
      661 acgggaaggg ctggtgggtg gcgagggatt cgccgtcgat gccagcttgg tggcggccga
      721 tgccagtcgt cagcgtggcg tgcccggcgg cgacgccgac tggtcggacc ggcgatcgat
      781 cagccgtccg gtgcgcgaat atctggcggc gctggatcag gatgaggtcg agaagaagac
      841 gcccgtgaac atttcgctaa ccgatccggc ggcgcgctgg agtgcggtat ggggaccggc
      901 gttctacgct tattcggcca actatctgat cgacatcgcc gcgggaattg tggtcgatgt
      961 cgaggcgacg ccctcccaca agctcgatga gatcaacgcc accaagacga tgatcgagcg
     1021 ggttgaggcg cgatttgatc tcaagccgcg gcggctgatc ggcgacaccg cttacggcac
     1081 ggccgagatg ctggcgtgga tggtcgacga gaagcagatc gaaccgcatg tatcggtgtg
     1141 ggaccggacc cagcgcgatg atggaaccct atccagcagt gacttccggt gggatccgga
     1201 gggcgacgaa taccactgtc cacagggcca ggtgctgcgg cgcgaacgac gggccttcaa
     1261 gacgccgcgt agccatgtta ccaaggccga cacgatcatc taccgcgcga gccaagccga
     1321 ttgcacagct tgcccgttga agaggcgttg ctgtccaaac acaccgaggc gcaagatcgc
     1381 gcgcagcatc tatgaatcag cgcgagacgt ggctcgcgag ctggccaaga cggacgcgta
     1441 caagcaatca cgcaaggacc gcaagaaggt cgaggtgctc ttcgctcacc tcaagcggat
     1501 cctgaagctt gatcgactgc ggttgcgcgg gcccagtggt gctcacgacg agtttctgct
     1561 tgcagcaacc gcgcagaacc tacgacgaat ggcgaagtgg ttgtacccgg ccgagccaga
     1621 aacagcaaat atggcgatct aaatcggcga agaacagcaa caccaaccca agatcgagcg
     1681 ccaccagtta ccgaacctct ctcttcgaag cgctcgcggc aatactgcat cgctgaaacg
     1741 tcgagttctt caacactatc agaagtgtgc cgacggtcgc cagcaccacc gcgcttgttg
     1801 cagaaaaagt accatctccc gcgtgggacc aataaccctt caccagccgg tattgcaaag
     1861 ccgcgactgg cgcagtgaaa tagccggaca tttgtcttgt cgacggctcg cgctgccgtt
     1921 ccaatagcgg cgcgccagac gcggcacgtt tccggcacgg gggtagtcgt ccctgccgcc
     1981 aagaagatgc gatccccgtc cgccgaacgt gccgaacccg actccgacgt gaacgcgtcg
     2041 gcgccggcac agcctctcgg cga
//
LOCUS       DBNHBY010000822         5619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1691104, whole genome shotgun sequence.
ACCESSION   DBNHBY010000822 DBNHBY010000000
VERSION     DBNHBY010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1691104"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="pnp"
                     /locus_tag="AC16HB_19105"
     CDS             <1..1358
                     /gene="pnp"
                     /locus_tag="AC16HB_19105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5608955.1"
                     /translation="KQARSARIEEAYSHLWAKLEEEAAASGQSMPDGNEIDGIMFDLQ
                     AKIVRSQILDGEPRIDGRDTRTVRPIAIRLGVLPRTHGSALFTRGETQALVTATLGTR
                     QDEQIIDALMGEYSERFMLNYTMPPFATGETGRVGAPKRREIGHGRLAKRALLACLPS
                     AEDFQYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGVAMGLIKEGNKF
                     AVLTDILGDEDHLGDMDFKVAGTVNGVTALQMDIKIEGITKEIMKVALDQAREGRLHI
                     LQHMQQAMEAPRGELSAFAPRLTKMKINPEKIRDVIGKGGAVIRALTEETGTQIDIED
                     DGTIIIASVDLEKAKEAERRIRELTAEVEVGKIYDGTVLRLLDFGAIVQILPGRDGLL
                     HISQIAAERVNQVSDYLKEGQAVKVKVIEADEKGRVRLSMKAAAAELGGAQQPQPAPQ
                     Q"
     gene            1373..2386
                     /locus_tag="AC16HB_19110"
     CDS             1373..2386
                     /locus_tag="AC16HB_19110"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR5608956.1"
                     /translation="MRAIEIARPGGPDALRLVDRPRPQPGSGEALIRVFAAGVNRPDV
                     LQRKGAYAPPPGASDLPGLEVAGVIEDGDLAGSDFAVGDAVCALVAGGGYAEYCVAPV
                     GQMLPVPRGVTFAEAAALPETHFTVWSNVFDRAHLAPGESLLVQGGSSGIGVAAIQIA
                     AALGNLVYATAGSDDKCRACEALGARRAINYRSEDFVAVVRELTARHGVDVILDMVAG
                     DYLPREIECLADDGRLVIIALLGGSKATLDCSAILRRRLTVTGSTLRPRPVEFKSAIA
                     RKLRARVWPLIEGGRVKPMLYRSFALADAAAAHALMESSQHVGKIVLDVTEQAAHRPQ
                     EHS"
     gene            2383..3132
                     /gene="tpiA"
                     /locus_tag="AC16HB_19115"
     CDS             2383..3132
                     /gene="tpiA"
                     /locus_tag="AC16HB_19115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5608957.1"
                     /translation="MTARRKLVAANWKMNGSTAANATWLDAFRVGRWACEVAVCAPFL
                     YVAQVRDGLSGLAAEPGAQNLATEAPGAFTGEVAAEMLVDVGCRWVIVGHSERRALYG
                     ETNDLVAAKAARALAVGLKPIVCIGETLAERDAGRTLDVVGAQLGAVLKRCTVAQIAR
                     GAVAYEPVWAIGTGRTATPAQAQEVHAAIRAQLVGADAGAGGAVRILYGGSVKPGNAK
                     ELFAQADIDGGLIGGASLVVADFLAICQAAG"
     gene            3151..3531
                     /gene="secG"
                     /locus_tag="AC16HB_19120"
     CDS             3151..3531
                     /gene="secG"
                     /locus_tag="AC16HB_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5608958.1"
                     /translation="MAYLTTLLIVLQVISAIAIIVLVLLQHGKGADMGAAFGGGASGS
                     LFGATGSANFLSRSTAVVATVFFIATLALAYTATSMRAVRAPQGGVMGTMPPAEPAKA
                     DAAPEAKKEAAPAGDASKAADIPK"
     gene            3567..3651
                     /locus_tag="AC16HB_19125"
     tRNA            3567..3651
                     /locus_tag="AC16HB_19125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3601..3603,aa:Leu,seq:gag)
     gene            3745..4110
                     /locus_tag="AC16HB_19130"
     CDS             3745..4110
                     /locus_tag="AC16HB_19130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5608959.1"
                     /translation="MNLDSYVPVLLFIVIGIVVGIAPIALGAALGPRKPDPEKLSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLGDLPQWGVAFWSMMLFL
                     AILTVGFLYEWKKGALDWE"
     gene            4118..4594
                     /locus_tag="AC16HB_19135"
     CDS             4118..4594
                     /locus_tag="AC16HB_19135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR5608960.1"
                     /translation="MAIEGILSEGFATTQVDKLINWSKTGSMWPMTFGLACCAVEMIH
                     AGASRYDLDRFGVMFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQNKIRRTNTIAR"
     gene            4614..5210
                     /locus_tag="AC16HB_19140"
     CDS             4614..5210
                     /locus_tag="AC16HB_19140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5608961.1"
                     /translation="MTRTDTLVDNLKRALGGRLASVTVALGEVTIVVKAADQHDAALT
                     LRDDPNLKFEQLIDLCGVDYQNYGNGAWEGARFAVVMHLLSVTHNVRVRVRTFCPDDE
                     LPLVASVTDVWNSANWFEREAFDLFGIVFEGHSDLRRILTDYGFVGHPFRKDFPISGH
                     VEVRYDPEKGRVVYQPVSIEPREITPRVVREDKYAGER"
     gene            5344..>5619
                     /locus_tag="AC16HB_19145"
     CDS             5344..>5619
                     /locus_tag="AC16HB_19145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5608962.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVVQRADPHIGLLH
                     RATEKLAENKTYIQSLPYMDRLDYMSMMCNEHAYCLAVEKLLQIEVPL"
BASE COUNT          950 a         1809 c         1975 g          885 t
ORIGIN      
        1 gcaagcaggc gcgctcggcc aggatcgagg aggcttacag ccacctgtgg gccaagctgg
       61 aggaggaggc ggctgcctcc gggcagtcga tgccggacgg aaacgagatc gacggcatca
      121 tgttcgacct gcaggcgaag atcgtgcgca gccagatcct cgacggcgag ccgcgcatcg
      181 acggccgcga tacgcgtacg gtgcgcccga tcgcgatccg gttgggcgtg ctgccgcgca
      241 cgcacggcag cgcactgttc acgcgcggtg agacgcaggc gctggtgacc gccacgctcg
      301 gcacccggca ggacgagcag atcatcgacg cgctgatggg cgagtacagc gagcgcttca
      361 tgctcaacta caccatgccg ccgtttgcca ccggcgagac cggccgcgtc ggtgcgccca
      421 agcggcgcga aatcggccat gggcggctgg ccaagcgcgc gttgctcgcg tgtctgccga
      481 gcgcggagga cttccagtac tcgatccgcg tggtgtcgga gatcaccgag agcaacggct
      541 cctcttcgat ggcttcggtc tgcggcggct gcctggcgct gatggatgcc ggcgtgccga
      601 tgaaggcgca cgtggccggc gtggcgatgg ggctgatcaa ggaaggcaac aagttcgcgg
      661 tgctgaccga catcctaggc gacgaggacc acctcggcga catggacttc aaggtcgccg
      721 gcacggtcaa tggcgtgacc gcgctgcaga tggacatcaa gatcgagggc atcaccaagg
      781 agatcatgaa ggtcgccctc gaccaggcgc gcgaaggccg gctgcacatc ctgcagcaca
      841 tgcagcaggc gatggaggcg ccgcgcggcg agctgtccgc gttcgcgccg cgtctgacca
      901 agatgaagat caaccccgag aagatccgcg acgtgatcgg caagggcggc gcggtgattc
      961 gcgcgctgac cgaggagacc ggcacgcaga tcgacatcga ggacgatggc accatcatca
     1021 tcgccagcgt cgatctggag aaggccaagg aagcggaacg ccgtatccgc gaactgaccg
     1081 ccgaggtcga ggtcggcaag atctacgacg gtaccgtgct gcgactgctg gacttcggcg
     1141 cgatcgtgca gatcctgccg ggacgtgacg gcctgctgca catctcgcag atcgcggccg
     1201 agcgcgtgaa tcaggtgtcc gattacctga aggaaggcca ggccgtgaag gtcaaggtga
     1261 tcgaagccga cgagaagggc cgcgtgcggc tgtcgatgaa ggcggctgcg gccgaactcg
     1321 gcggcgcgca gcagccgcag ccggcgccgc agcagtagcc gggagtcgcg cggtgagggc
     1381 gatcgagatc gctcggcccg gcgggcccga cgcgctgcgc ctcgtcgatc gaccgcggcc
     1441 gcagcccggc tcgggtgagg cgctgatccg cgtgtttgcc gccggcgtga accgccccga
     1501 cgtgctgcag cggaagggcg cctacgcgcc accgccggga gcgtccgatc tgcccggact
     1561 ggaggtcgcg ggcgtgatcg aggacggcga tctcgccggc agcgactttg cggtgggaga
     1621 cgcggtatgc gcgctggtcg cgggcggcgg ctacgccgaa tactgcgtgg cgccggtcgg
     1681 ccagatgctg ccggtgccgc gcggcgtgac attcgccgag gcggcggcgc tgcccgaaac
     1741 gcacttcacg gtgtggagca acgtgttcga tcgcgcgcac ctggcgccgg gggaatctct
     1801 gctcgtgcag ggcggctcga gcggcatcgg cgtggcggcg atccagatcg ctgcggccct
     1861 cggcaacctg gtgtacgcga ccgcgggcag cgatgacaag tgccgcgcct gcgaggcgct
     1921 cggcgcgcgc cgcgcgatca actaccgcag cgaggatttc gttgcggtgg tgcgcgaact
     1981 gaccgcgcgg cacggcgtcg acgtgatcct cgacatggtc gcgggcgatt acctgccgcg
     2041 cgagatcgag tgcctggccg acgatggccg actggtgatc atcgcgctgc tcggcggctc
     2101 caaggccacg ctcgactgca gcgcgatctt gcgccggcgc ctgacggtga ccggctcgac
     2161 gctgcggcca cgcccggtcg agttcaagtc cgcgatcgcg cgcaagctgc gcgcgcgcgt
     2221 gtggccgctg atcgaaggcg gccgcgtgaa gccgatgctc tacaggtcgt tcgcgctggc
     2281 ggacgcagcg gccgcgcacg cactgatgga atcgagccag catgtcggca agatcgtgct
     2341 ggacgtcacg gaacaggccg cgcatcggcc gcaggagcac tcatgaccgc gcgccgcaag
     2401 ctggttgccg ccaactggaa gatgaacggc tcgaccgcgg ccaacgcgac gtggctcgac
     2461 gcgtttcgtg tcgggcgctg ggcctgcgaa gtggcggtgt gtgcgccctt cctttatgtg
     2521 gcgcaagtgc gcgacgggct gtcgggactg gcggcggagc ccggcgcgca gaacctcgcg
     2581 acggaggcac caggcgcgtt cacgggcgaa gtcgcggccg agatgctggt agacgtcggc
     2641 tgcaggtggg taatcgtcgg ccactcggag cggcgcgccc tgtatggcga gaccaacgac
     2701 ctcgtcgccg ccaaggccgc gcgcgcattg gcggtcggac tgaagcccat cgtgtgtatc
     2761 ggcgaaacgc tcgccgagcg cgatgccggt cgcacgctcg acgtcgtcgg cgcgcagctc
     2821 ggcgccgtgc tgaagcgctg caccgtcgcc cagatcgcgc gcggcgcagt tgcctatgag
     2881 ccggtgtggg cgatcggtac cggtcgcacc gccaccccgg cgcaggcaca ggaggtgcac
     2941 gcagcgatcc gcgcacagct cgtcggcgcc gacgcgggcg cagggggcgc tgtgcgcatc
     3001 ctgtatggcg gcagcgtgaa acccggcaac gcgaaggagc tcttcgcgca ggccgacatc
     3061 gacggcggac tgatcggcgg cgcgtcgctg gtcgtggcgg acttcctggc gatctgccag
     3121 gccgccggct gaacttcgag aggaaaccga atggcgtatc tgacgacatt attgatcgtg
     3181 ctgcaggtga tcagtgccat cgcgatcatc gtgctggtac tgcttcagca cggcaagggc
     3241 gccgacatgg gcgccgcgtt cggcggcggc gcgtcgggca gtctgttcgg cgccaccggc
     3301 tcggcgaact tcctgtcgcg cagcacggca gtcgttgcga ccgtgttctt catcgcgacc
     3361 ctggcgctcg cgtacaccgc cacctcgatg cgcgcggtgc gcgcgccgca gggcggcgtg
     3421 atgggaacga tgccgccggc cgagccggcc aaggccgacg cggcaccgga agcgaagaag
     3481 gaggccgcgc ccgcgggcga tgcgagcaag gcggccgaca ttccgaagta aacgaggcag
     3541 gtgattcaag tagaatccgc gccgatgccg acgtggtgga attggtagac acaccatctt
     3601 gagggggtgg cggcgcaagc cgtgtgagtt cgagtctcac cgtcggcacc atccgtcgag
     3661 catcgggtca aaggtttggc ggcacgaccg caatcgaccc agcggcccag tacgcatatc
     3721 cgccagcgcc ctgatggacc gggaatgaat ctcgacagtt acgtccctgt actcctgttc
     3781 atcgtgatcg gaatcgtcgt cggcatcgcg cccatcgcgc tcggcgcggc gctcggcccg
     3841 cgcaagcccg atccggagaa gctgtcaccg tacgaatgcg gcttcgaggc gttcgaggac
     3901 gcgcgcatga agttcgacgt gcgctattac ctcgtcgcca ttctgttcat cctgttcgac
     3961 ctcgagatcg cgttcctctt tccgtgggct gtcgtgctcg gcgacctgcc gcagtggggc
     4021 gtggcgttct ggtcgatgat gcttttcctg gcgatcctga cggtcggttt cctgtacgag
     4081 tggaagaagg gcgcactgga ctgggaatga gcgcgtcatg gcgatcgaag gcatcctgag
     4141 cgaaggcttc gcgaccacgc aggtcgacaa gctgatcaac tggagcaaga ccgggtcaat
     4201 gtggccgatg acgttcggcc tcgcgtgctg cgcggtcgaa atgatccacg ccggcgcgtc
     4261 gcgttacgac ctcgaccgtt tcggcgtgat gttccgtccc agcccgcgcc agtcggacgt
     4321 gatgatcgtc gccggcacgc tctgcaacaa gatggcgccg gcgctgcgta aggtgtacga
     4381 ccagatggcc gagccgcgct gggtgatcag catgggctcg tgcgccaacg gcggcggcta
     4441 ttaccactac agctactcgg tcgtgcgcgg ctgcgatcgc atcgtgccgg tcgacgtcta
     4501 cgtccccggc tgtccaccga cggccgaggc cctgatctac gggatcatcc agctgcagaa
     4561 caagatccgc cggaccaaca cgatcgcgcg ctgaccgcga tcgcggacat gagatgaccc
     4621 gcaccgacac cctggtcgac aacctgaagc gcgcgctcgg cgggcggctc gccagcgtca
     4681 ccgtcgcgct cggcgaggtc acgattgtcg tcaaggcggc cgatcaacac gatgcggcgc
     4741 ttacgctgcg cgacgacccg aacctcaagt tcgagcagct gatcgacctc tgcggcgtcg
     4801 attaccagaa ctacggtaac ggcgcctggg aaggcgcgcg cttcgctgtc gtgatgcacc
     4861 tgctgtcggt cacgcacaac gtgcgcgtgc gcgtgcgcac gttctgtccc gacgacgagc
     4921 tgccgctggt ggcgtcggtt accgacgtgt ggaattcagc caactggttc gagcgcgagg
     4981 cgttcgacct gttcggcatc gtcttcgagg gccacagcga cctgcgccgg atcctcaccg
     5041 actatggttt cgtcggccat ccgttccgca aggatttccc gatctcgggc cacgtcgagg
     5101 tccgctacga ccccgaaaag gggcgggtcg tctaccagcc ggtgtcgatc gagccgcgcg
     5161 agatcacgcc ccgcgtcgtg cgcgaggaca aatacgcggg cgagcgctga tcgatggccg
     5221 agattcgcaa ctacacgatg aattttggcg tccacataac gtcggcggcg ccgacattag
     5281 tgtccgcctc aacaagccgc ttcgctttcg ctcgcggcgt gttgcggacc cggcccgaaa
     5341 ttcatggctg aaattcgtaa ctacaccatg aatttcgggc cccagcatcc ggctgcgcac
     5401 ggcgtgctgc ggctcgtgct cgagctcgac ggcgaggtcg tgcagcgcgc cgacccgcac
     5461 atcggactgc tgcaccgcgc gaccgagaag ctggccgaaa acaagacgta tatccagtcg
     5521 ctgccgtaca tggaccggct cgactacatg tccatgatgt gcaacgagca cgcctactgc
     5581 ctcgcggtcg agaagctgct gcagatcgag gtgccgttg
//
LOCUS       DBNHBY010000823         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_269906, whole genome shotgun sequence.
ACCESSION   DBNHBY010000823 DBNHBY010000000
VERSION     DBNHBY010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_269906"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC16HB_19150"
     CDS             <1..707
                     /locus_tag="AC16HB_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR5608963.1"
                     /translation="LAAMTGYRNENSQGHIITIEDPIEYVHPHKNCIITQREVGVDCD
                     SWQVALKNTLRQAPDVILIGEVRDRETMEHAIAFSETGHLVMCTLHANSTNQALDRVI
                     NFFPEEKRQQLLMDISLNLRAMISQRLIPFKDRKGRIPAVEIMLNSPLISELIFKGDV
                     PEIREVMKRSREQGMQTFDQALYDLHEKELISYEDALRNADSVNDLRLQIKLNSKLYG
                     GVQGIHKGIEHLGLSS"
     gene            832..1317
                     /locus_tag="AC16HB_19155"
     CDS             832..1317
                     /locus_tag="AC16HB_19155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR5608964.1"
                     /translation="MPSVHEFSATTIDGKSRKLADYHDKVLLIVNVASHCGFTPQYKG
                     LEALYEKYRKQGLVVLGFPCNQFGAQEPGTESEIAAFCEKNYGVTFPLFAKVDVNGAN
                     AHPLYKHLSATAPGLLGSESIKWNFTKFLVDRKGNVLKRYAPNTAPADLEKDIEKALT
                     A"
     gene            complement(1318..>2433)
                     /locus_tag="AC16HB_19160"
     CDS             complement(1318..>2433)
                     /locus_tag="AC16HB_19160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR5608965.1"
                     /translation="ELRALGAQPAQPEPGGVAFAGTLATAYAANLHSRLASRVLWEVC
                     RRGYASEDDLYAAAQAVAWEAHFDARQTLRVDVTATRSPLRSLEFATLRIKDAIVDRL
                     RAKTGARPSIDRARPDVRVFAHLDATHLTLYLDLSGEPLFKRGWRADKGEAPLKENLA
                     AGLLLLAGWTPERPLLDPFCGSGTIVIEAAQIASKRAPGLARTFGFEKLHGCDRRAWE
                     ALREAARARIDDRAGAHIAGSDISTRVIAQAVANAELAGLRAWLTDGRLTLAARDART
                     AAPLGAHGMIVSNPPYGEQSAPRSASVPRMMADIGDRLKQSFAGWEAWFLTSDRALPR
                     QMRLAETRKTVLYNGPLECRFFRFELVEGSYRPRATQ"
BASE COUNT          428 a          802 c          821 g          382 t
ORIGIN      
        1 cgctcgcggc gatgaccggc tatcgcaacg agaattcgca gggccacatc atcacgatcg
       61 aggacccgat cgagtacgtg catccgcaca agaactgcat catcacgcag cgtgaggtgg
      121 gggtcgactg cgatagctgg caggtggcgc tgaagaacac gctgcgccag gcgcccgacg
      181 tgatcctgat cggcgaggtg cgcgaccgcg agacgatgga gcacgcgatc gcattctccg
      241 agaccggcca tttggtgatg tgcacgctgc acgccaactc gaccaaccag gcgctcgacc
      301 gcgtgatcaa cttctttccg gaagaaaagc gccagcagct gctgatggac atttcgctga
      361 acctgcgcgc gatgatttcg cagcggctga ttccgttcaa ggaccgcaag ggtcggattc
      421 cggcggtcga gatcatgctc aattcgccgc tgatatcgga gctgatcttc aagggcgacg
      481 tgcccgagat ccgcgaggtc atgaagcgtt cgcgcgagca gggcatgcag accttcgacc
      541 aggcgctgta cgatctgcac gagaaggaac tgatctccta cgaggacgcg ctgcgcaatg
      601 ccgactcggt caacgacctg cggctgcaga tcaagctgaa ctcgaagctg tacggcggcg
      661 tgcagggaat ccacaagggg atcgaacatc tcggcctctc cagctagcgc tggcgagtcc
      721 gcgctgttcg tgaacaggtc gggccctctc tccgccgacc cctccctctc cgggaggggc
      781 aaaggcagac gcaaacgcga gactgcgcgc aactcacttg ggagttgcgc gatgccatcc
      841 gtccacgagt tttccgcgac gacgatcgac ggcaagtccc gcaagctcgc ggactaccac
      901 gacaaggtgc tgctgatcgt caacgtcgcg agccactgcg ggttcacgcc gcaatacaag
      961 gggctcgagg cgctgtacga gaagtaccgc aagcagggtt tggtggtgct gggattcccg
     1021 tgcaaccagt tcggcgcgca ggagccgggg actgaatcgg agatcgcggc gttctgcgag
     1081 aagaactacg gcgtcacgtt tccgctgttc gccaaggtcg atgtcaacgg cgccaacgcg
     1141 catccgctgt acaagcacct gagcgccacg gcgcccgggc ttctcggcag cgagtcgatc
     1201 aagtggaact tcaccaagtt cctggtcgac aggaagggca acgtgctgaa gcgctacgcg
     1261 ccgaacaccg caccggcgga tctggagaag gacatcgaga aggcgctgac cgcctgatta
     1321 ctgggtcgcg cgcggccggt aactgccttc gaccagttcg aagcggaaga accggcattc
     1381 gagcgggccg ttgtagagca cggtcttgcg tgtctccgcg agccgcatct ggcgcggcag
     1441 cgcgcggtcg gaggtcagga accacgcctc ccagccggcg aacgactgct tcagccggtc
     1501 gccgatgtcg gccatcatcc taggcaccga cgccgagcgc ggcgcgctct gttcgccgta
     1561 cggcgggttg ctgacgatca tcccgtgcgc gccgagcggc gcggcggtgc gtgcgtcgcg
     1621 cgcggcgagc gtcaggcggc cgtcggtgag ccacgcccgc agcccagcga gttcggcgtt
     1681 ggcgaccgcc tgcgcgatga cccgggtcga aatgtcgctg ccggcgatgt gcgcgccggc
     1741 gcggtcgtcg atgcgcgcgc gcgctgcctc gcgcagcgct tcccacgcgc ggcggtcgca
     1801 gccgtgcagc ttctcgaacc cgaatgtgcg cgccagcccc ggcgcgcgct tggaagcgat
     1861 ctgcgcggct tcgatcacga tcgtgccact gccgcagaac ggatcgagga gcggtcgctc
     1921 cggcgtccag ccggccagca gcagcaatcc cgccgcaagg ttttccttca gcggtgcctc
     1981 gcccttgtcc gcacgccagc cgcgcttgaa gagcggttcg cccgacaggt ccaggtacag
     2041 cgtgaggtgc gtggcgtcga gatgcgcgaa cacgcgcacg tcagggcgtg cgcggtcgat
     2101 cgacggccgc gcgccggtct ttgcgcgcag ccggtcgacg atcgcgtcct tgatccgcag
     2161 cgtggcgaac tccagactgc gcagcggcga acgcgtcgcg gtcacgtcga cgcgcaacgt
     2221 ctggcgcgcg tcgaagtgcg cctcccatgc gaccgcctgc gcggcagcgt acaggtcgtc
     2281 ctcgctcgcg tagccacggc ggcacacttc ccacagcacc cggctggcca ggcgcgaatg
     2341 caggttggct gcgtacgcgg tggcgagcgt gcctgcgaac gcgacgccgc caggctccgg
     2401 ctgcgccggt tgcgcgccga gtgcgcgcag ttc
//
LOCUS       DBNHBY010000824         6402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1419700, whole genome shotgun sequence.
ACCESSION   DBNHBY010000824 DBNHBY010000000
VERSION     DBNHBY010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1419700"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="AC16HB_19165"
     CDS             <1..1006
                     /locus_tag="AC16HB_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5608966.1"
                     /translation="KIVPLTQANVCASAYNIVETLRLTPADRELSIMPLFHIHGLIAG
                     LLAPLAAGSTIFCSPGFNALKFFAWMAECKPTWYTAVPTMHQTILARAPQNREIIAAN
                     PLRFLRSSSSSIPPQVIAELEQVFNAPLVEAYGMTEASHQMASNPLPPAARKPGTVGR
                     AAGPEIAIMDEAGNLLMAGATGEIVIRGPNVTAGYENNPKANGEAFTNGWFRTGDQGT
                     MDPEGYLSITGRLKEIINRGGEKISPREVDEVLMDHPAVAQVVTFGIPHDKLGEDVGA
                     VVVLREGRQATERELRDFVGKRLADYKVPRKILFMNEIPKGATGKLQRIGLAQKLGLS
                     "
     gene            complement(1193..1843)
                     /locus_tag="AC16HB_19170"
     CDS             complement(1193..1843)
                     /locus_tag="AC16HB_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5608967.1"
                     /translation="MAIDPGSPALQQIRDPMVRELALRGVLRNYAAGAHIIKEGDKGD
                     SLFVILSGKVKVYVSDRDGRDMVLDFHGPGDYIGEMALDGKPRSASVAAVEPTVCSIV
                     MRDALRAAIAANPDVAMTLIATLIERARIATDNVKNLALMDVYGRVARLLLAIAQEQP
                     DGRLVVPERLTQQDIAERVGSSRDMVSRIFKDLTAGGYITVVDRQITIHRKPPARW"
     gene            complement(1879..2697)
                     /locus_tag="AC16HB_19175"
     CDS             complement(1879..2697)
                     /locus_tag="AC16HB_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5608968.1"
                     /translation="MHWPLLAALLALGSATGFVAGLLGVGGGMLMVPFLTMLFTAQSF
                     PSQHIVHMAIATSLATILFTSVSSVRAHHQRGAVQWRIVQFLAPGILLGSLIGAQLAG
                     RLPTAWIALLFALFVGFSATQMLIGRKPRPQRELPPPAGMFGVGGAIGLASSIVGAGG
                     GFISIPFMARCNVTIHKAVATSAALGFPIAAAGAVGYAIAGWAEPWLPPGSLGFIYVP
                     ALLAIVAGSVLTAPLGARVAHATDTRRLSRIFAALLYSLSGYMLYKSYSAWGAA"
     gene            complement(2697..3149)
                     /locus_tag="AC16HB_19180"
     CDS             complement(2697..3149)
                     /locus_tag="AC16HB_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR5608969.1"
                     /translation="MKSEPDECSIDDALTAKDSTVPWTGVRNYQARNYMRDAMKVGDG
                     VLFYHSGCAEPGIAGIAEVARAAYPDATQFDPKSAYYDPKSTRAAPRWVHVDVRALRK
                     TRLISLAELRAAPALADMVVLKRGNRLSITPVSASEWRFVTTRLAKTR"
     gene            complement(3224..3404)
                     /gene="ssrS"
                     /locus_tag="AC16HB_19185"
     ncRNA           complement(3224..3404)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16HB_19185"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3411..3719)
                     /locus_tag="AC16HB_19190"
     CDS             complement(3411..3719)
                     /locus_tag="AC16HB_19190"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR5608970.1"
                     /translation="MASEQQLTLTIFGRDYRVACAPEERDDLIACARYVDMKMTAIRD
                     TGKVMGADRVAVMAALQLAQELFAAKATGGVSMGDLRRRLKQLNEVADEMLAPQEKLF
                     "
     gene            complement(3734..3967)
                     /locus_tag="AC16HB_19195"
     CDS             complement(3734..3967)
                     /locus_tag="AC16HB_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HIR5608971.1"
                     /translation="MLEDLDALTAKMTELSGRMRALREENQRLRTQLATANVELDTLR
                     GRVAGAMERIDALLARLPPPAEKNAESAPRTGS"
     regulatory      3995..4230
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4363..6180
                     /locus_tag="AC16HB_19200"
     CDS             4363..6180
                     /locus_tag="AC16HB_19200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5608972.1"
                     /translation="MHLHRFMPPALAVVFVFSTSTSIAQTAPQSLGPVIVSAARIEQP
                     LAEALPNTSVITRVDIERSQAPDLLTLLARLPGVEVAQLGGIGSQAGLFLRGGETRHV
                     LVLIDGVPMNNLNFSLASLDQIMAAQVERVELVRGNVSSLYGSQAVGGVISVFTRTDG
                     SGIDARAGIGSRGTNEVQAGARGGAGGWRYSIALSGLHTDGFDQLDQTERPGTNPDRD
                     GYRNESASARLSYSWAQDQSLSVHGLRTRGRLQYDSEFGPATQADESVQTIETFGAVA
                     RNRLAANWVSQLAVSRLRDKLDAEVTAFPFFVTSTGTLAAWQNEIDLDQWKATLAAER
                     LRQTIDSDTKYNVSGRTVNTLRAGLLGNFGAHQLQLNARRDDYSDFGVANTGYVGYGY
                     PVTSALKLIASASTAFNAPTFNDLFYPFGGNPDLKPERTRSVEAGVQYRAGATSVRLQ
                     LFRTRFRDLIGFDAAFNRVNIGRATTQGAEFSFDAASGGWRAQVAATLQDATDGDTGH
                     RLVRRARSFGSLLLSRALGAVDVQANLRATGDRRDRALGSDHILGGYLLVDLAARWRV
                     QRDLLLTLRVENAFDRAFENAYGYRGTPRGVFGGIELHL"
     gene            6177..>6402
                     /locus_tag="AC16HB_19205"
     CDS             6177..>6402
                     /locus_tag="AC16HB_19205"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR5608973.1"
                     /translation="MKTRAAPTLIVLALAGVASVAAALTIGSVRVPAHDLWVALFGAN
                     DTSLAAIVFDLRLPRALSAFATGALLALSGA"
BASE COUNT         1053 a         2143 c         2215 g          991 t
ORIGIN      
        1 gaagatcgtg ccgctgacgc aggccaacgt gtgcgcctcg gcctacaaca tcgtcgagac
       61 gctgaggctc acgcccgcgg accgggagct ttcgatcatg ccgctgttcc atatccacgg
      121 cctgatcgcg gggctgctgg cgccgcttgc cgccggctcg acgatcttct gctcgccggg
      181 cttcaacgcg ctgaagttct ttgcgtggat ggccgagtgc aagccgacct ggtacacggc
      241 ggtgccgacg atgcaccaga cgatcctcgc acgcgcgccg cagaaccggg agatcatcgc
      301 ggccaacccg ctgcgctttc tgcgctcgtc gagttcatcg attccgccgc aggtgattgc
      361 ggagttggag caagtcttca acgcgccgct ggtcgaggcc tatggcatga ccgaggcctc
      421 gcaccagatg gcgagcaatc cgttgccgcc cgcggcgcgc aagccgggca cggtcggccg
      481 cgcggccggc ccggagatcg cgatcatgga cgaggcgggt aacctgctga tggcgggcgc
      541 gaccggagag atcgtgattc gcggcccgaa cgtcactgct ggctacgaga acaacccgaa
      601 ggcgaatggc gaggcgttca ccaacggctg gttccgcacc ggcgaccagg gcacgatgga
      661 ccccgaaggg tatctgtcga tcacgggacg gctgaaggag atcatcaatc gcggcggcga
      721 gaagatctca ccgcgcgagg tcgacgaggt gctgatggac cacccggcgg ttgcgcaggt
      781 ggtgaccttc gggatcccgc acgacaagct cggggaagat gtcggcgcgg tggtcgtgct
      841 gcgagaaggc aggcaggcga ccgagcgtga actgcgcgac ttcgtcggca agcggcttgc
      901 cgactacaag gtgccgcgca agatcctgtt catgaacgag atcccgaaag gcgccacggg
      961 caagctgcag cggatcgggc tggcgcagaa gctggggctg tcctgagcgc aacatgacaa
     1021 cctcctgcgt cttgcgcaag agaggcgtcg cggcggcaat cagatcagca gcatcggata
     1081 cgtgatcgat caggagccca gagtctgcgc tgaaggcgcg gcgcagggca cgtgaatatc
     1141 cgacagccgc gatcccatga acgcggggaa acgggtactc accaaccggg catcaccagc
     1201 gcgccggcgg cttgcggtgg atggtgatct ggcggtcgac gaccgtgatg tagccgccgg
     1261 cggtcaggtc cttgaagatg cggctcacca tgtcgcgcga ggagccgacc cgctcggcga
     1321 tgtcctgctg cgtcaaacgc tccggcacga ccagccggcc gtcgggctgc tcctgcgcga
     1381 tcgcgagcag cagccgcgcg acgcgtccgt agacgtccat cagcgcgaga ttcttcacgt
     1441 tgtcggtggc gatgcgcgca cgctcgatca gcgtcgcgat cagcgtcatc gccacgtccg
     1501 gattggccgc gatcgctgcg cgcagcgcgt cgcgcatgac gatcgagcac acggtcggct
     1561 cgacggccgc caccgacgcc gagcgcggct tgccgtcgag cgccatctcg ccgatgtagt
     1621 cgccggggcc atggaagtcg agcaccatgt cgcggccgtc gcggtccgac acgtagacct
     1681 tcaccttgcc gctgaggatc acgaacagcg aatcgccctt gtcgccttcc ttgatgatgt
     1741 gtgcgccggc cgcgtagttg cgcagcaccc cgcgcagggc gagttcgcgc accatcggat
     1801 cgcgaatctg ctgcagtgcc ggactgccgg ggtcgatcgc catcctgtcg tcttctcagg
     1861 tatcgaggcc ggccgtcatt atgcggcgcc ccaggcgctg tacgacttgt aaagcatgta
     1921 gcccgacagc gaatagagca gggcggcgaa gatgcgcgac agtcgccgcg tgtcggtggc
     1981 gtgcgccacg cgtgcgccca gcggcgcggt cagcacgctg ccggcgacga tcgccagcag
     2041 cgccggcacg taaatgaagc cgagcgatcc cggcggcagc cagggctcgg cccagccggc
     2101 gatcgcgtag ccaacggcgc ccgcggcggc gatcggaaac ccgagtgccg cggacgtcgc
     2161 caccgccttg tggatggtca cgttgcagcg ggccatgaat gggatcgaga tgaacccgcc
     2221 gccggcgccg acgatgctcg acgccagccc gatcgcgcca cccacgccga acatgccggc
     2281 cggcggcggc agttcgcgct gcggcctggg cttcctgccg atcagcatct gcgtcgcaga
     2341 gaagccgacg aagagcgcga acagcagcgc gatccatgcc gtgggcaggc gccccgccaa
     2401 ctgcgcgccg atcagcgagc cgagcaggat cccgggcgcc aggaactgca cgattcgcca
     2461 ttgcaccgcg ccgcgctggt ggtgcgcgcg gacggacgac accgatgtga agagaatcgt
     2521 cgccaacgag gtcgcgatcg ccatgtgcac gatgtgctgg gacgggaagc tctgcgcggt
     2581 gaacagcatc gtcaggaacg gcaccatcag catccctccg ccgacgccca gcaggcccgc
     2641 cacgaaaccg gtcgccgatc cgagcgccag cagcgcggcg agcagcggcc agtgcatcag
     2701 cgtgtcttgg cgagccgggt ggtgacgaag cgccattcgc tcgcggagac cggcgtgatc
     2761 gaaagccggt tgccgcgctt gagcaccacc atgtcggcga gcgcgggcgc ggcgcgcagt
     2821 tcggcgagcg agatgaggcg cgtcttgcgc agcgcgcgca cgtcgacgtg cacccagcgt
     2881 ggcgctgcgc gggtggactt cgggtcgtag tacgcgctct tcggatcgaa ctgcgtggcg
     2941 tcgggatacg ccgcgcgcgc gacctccgcg atgccggcaa ttccgggctc ggcgcagccg
     3001 gagtgataga agagcacgcc gtcgccgacc ttcatggcgt cgcgcatgta gttgcgcgcc
     3061 tggtaattgc gtacgccggt ccacggcacg gtcgagtcct ttgccgtgag cgcatcgtcg
     3121 atcgagcact cgtccggctc cgacttcatc aaccagtagc gcaccgagga ccatgaaaaa
     3181 agaagtgcgc ggttcgaagt aaccgaaccg cgccctcctg gagtaaatcc ccgcgcatgc
     3241 cgcccggcct gtgtcctgaa ccatcggttc agggtggtcg ctgtccacgc cgcttaggtt
     3301 cagcagacgc gtgccgatcg caccacggcg cgagcccagc aaccttgcgc aatgcgcatc
     3361 ggttcaagga acgagaggcc tttgcgcgaa caccgcaggg agaccgaatc ttagaacagc
     3421 ttctcctgtg gcgcgagcat ctcgtcggcg acttcgttca gctgcttcaa gcggcgccgc
     3481 aggtcgccca tcgacacgcc gccggtggcc ttggccgcga acagttcctg cgccaattgc
     3541 agcgcggcca tgaccgcgac gcggtccgcg cccatcacct tgccagtgtc gcggatcgcg
     3601 gtcatcttca tatcgacata gcgggcgcac gcgatcaggt cgtcgcgctc ttccggcgcg
     3661 caggcgacgc ggtagtcgcg gccgaagatc gtcaaggtga gctgctgctc gctggccaat
     3721 cgatgctccc cgatcacgaa ccggtgcgcg gcgccgattc ggcgttcttc tccgccggcg
     3781 gcggcaggcg cgcgagcagc gcgtcgatgc gctccatcgc gccggcgacg cgcccgcgca
     3841 gcgtgtcgag ctcgacgttc gccgtggcga gctgcgtgcg cagccgctgg ttctcctccc
     3901 gcagcgcgcg catgcggccg gacaactcgg tcatcttcgc ggtcaatgca tcgaggtctt
     3961 cgagcatgcg gaaatggtag caccggggcg ctgctagact tcagtcgcct ttggtgcctc
     4021 gacagtcatg cgtcgaggtg aaacgggaaa cagggatgcg gctcgtttga gtcgggtcgc
     4081 aacaacctgt gctgcccccg caacggtaag cagatgccgg cgcattggcg ccggtgcgct
     4141 tcacgatcaa ccactgcgcg gccgtcgctg cgcgggaagg tgtgaaggac gctctgcagc
     4201 ccggagaccg gccgaaggtc tcgccgtcga ccgtgcatgc cctgcgtcga cggcgtgccg
     4261 gtggacctgc cacggggagg tggcggtgcg ggcatcgcac cttcctcgcg ctcgtccggt
     4321 tgtctgttcc cggcggtggg ccggatcgag atcgaggaag caatgcacct gcatcgtttc
     4381 atgccgcccg cactcgcggt ggtattcgtc ttttccacct ccacttcgat cgcgcagacc
     4441 gcgccgcaat cgctcgggcc ggtgatcgtc agcgccgccc gcatcgaaca gccgctcgcc
     4501 gaggcgctgc cgaacaccag cgtgatcacg cgcgtggaca tcgagcgcag ccaggcgccc
     4561 gatctgctga cgctgctggc gcggctgccc ggcgtcgagg tcgcgcaact gggcggaatc
     4621 ggcagccaag cgggactgtt cctgcgcggc ggcgaaacgc gtcacgtgct ggtgctgatc
     4681 gacggcgtgc cgatgaacaa cctgaatttc tcgctcgcgt cgctcgacca gatcatggcg
     4741 gcacaggtcg agcgcgtgga actcgtgcgg ggcaacgtca gcagcctgta cggttcgcag
     4801 gcggtgggcg gcgtgatctc ggtgttcacg cgcacggatg gcagcggcat cgacgcgcgc
     4861 gcgggcatcg gtagccgcgg caccaacgaa gtgcaggcgg gagcgcgcgg cggcgctggc
     4921 ggctggcgct acagcatcgc attgtcgggc ctgcacacgg acggcttcga ccagctcgat
     4981 caaaccgagc ggcccggcac caaccccgac cgtgacggct atcgcaatga gagcgcgtcg
     5041 gcgcggctga gctattcgtg ggcgcaggat cagtccttga gcgtgcacgg cctgcgcacg
     5101 cgcggccggc tgcagtacga cagcgagttc ggccccgcga cgcaggccga cgaatcggtg
     5161 cagacgatcg agacgttcgg cgcggtcgcg cgcaaccgcc tcgcagcgaa ctgggtgtcg
     5221 cagctcgcgg tcagccgcct gcgcgacaag ctcgatgccg aagtgaccgc gttcccgttc
     5281 ttcgtcacca gcaccggcac gctggccgcg tggcagaacg agatcgacct cgatcaatgg
     5341 aaggcaacgc tcgcggccga gcgcctgcgg cagacgattg acagcgacac caagtacaac
     5401 gtatccgggc gcacggtgaa cacgctgcgc gcgggtctgc tgggcaattt tggcgcgcat
     5461 cagctgcagc tgaacgcgcg ccgcgacgac tactccgatt tcggcgtcgc gaacacgggg
     5521 tacgtcggct acggctaccc agtcacgagc gcgctgaagc tgatcgccag cgcatcgacc
     5581 gcattcaacg cgccgacgtt caacgatctg ttctatccgt tcggcggcaa tccggatctc
     5641 aagccggagc gcacccgatc ggtcgaggcc ggagtgcagt accgcgccgg tgccacgtcg
     5701 gtgcggctgc agctgtttcg cacgcgcttt cgggacctga tcgggttcga tgcggcattc
     5761 aatcgcgtga acattggccg cgcgacgacg cagggcgccg aattctcgtt cgacgccgcg
     5821 tccggcggct ggcgcgcgca ggtcgcggcc acgctgcagg acgcgaccga cggcgacacc
     5881 ggccaccggc tcgtgcgccg cgcgcgctcg ttcggcagcc tgctgctgtc gcgcgcgctc
     5941 ggcgcggtcg acgtgcaggc gaacctgcgt gcaacaggcg atcggcgcga ccgcgcgctc
     6001 ggcagcgatc acatcctggg tgggtatctg ttggtcgacc tcgcggcgcg ctggcgcgtg
     6061 cagcgcgatc tgctgctgac gctgcgcgtg gagaacgcct tcgaccgcgc gttcgagaac
     6121 gcatacggct accgcggcac gccgcgcggt gtgttcggcg gcattgaact gcacctgtga
     6181 aaacgcgcgc cgctccgact ctgatcgtgc tcgcactggc cggcgtggcc agcgtcgcgg
     6241 ccgcgctcac gatcggcagc gtgcgggtgc cggcgcacga cctgtgggtg gcgttgttcg
     6301 gcgcgaacga cacgtcgctg gcggcgatcg tgttcgacct gcggctgccg cgcgcgctgt
     6361 cggcgttcgc caccggcgcg ctgctcgcgc tgtcgggcgc ga
//
LOCUS       DBNHBY010000825         4211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1486872, whole genome shotgun sequence.
ACCESSION   DBNHBY010000825 DBNHBY010000000
VERSION     DBNHBY010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1486872"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="AC16HB_19210"
     CDS             complement(<1..1199)
                     /locus_tag="AC16HB_19210"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR5608974.1"
                     /translation="MDLNRLERIDDFCWRVKLPAETQGTALLYGDRALVAAMDDKVLE
                     QIGNVAQLPGLVGPAMAMPDAHWGYGFPIGGVAAFDPDRGGIISAGGVGFDISCGIRC
                     LRTSLTLDDLLPKSGKLGDSLFRDIPAGVGVEGDLQLDDADLDDIMIEGAEWAVRHGY
                     GTGAELEFVEERGRIRGAVPDSVSNTAKKRQRGEMGTLGSGNHYLEVQVVERIHDEKA
                     AQAFGVREGQIVVSIHCGSRGLGHQIGTDYLVSLAKAASRLGIHLPDRELACAPINSP
                     EGQEYLGAMNAAINVALANRQILAHIARRTLAHFFPQASVDTLFDVSHNTCKLEQHEV
                     ERKRRWLYVHRKGATRAFGPGHPAVPLPYRDVGQPVIIGGSMGTGSYILAGTHESEQR
                     AFSSASHG"
     gene            complement(1207..1635)
                     /locus_tag="AC16HB_19215"
     CDS             complement(1207..1635)
                     /locus_tag="AC16HB_19215"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR5608975.1"
                     /translation="MSATNDSTDYFEHAADIGVIGRGASMERAFEAAARAMFAIMSER
                     SAAEPAAAITAQIEITEDDVELALVRWLNALLAAAREQRGVFFEFHVVRDGAHWRGEA
                     RGRRWQPDEERGTEVKGATLTALAVRPSAAGWEARCVVDV"
     gene            1762..3279
                     /locus_tag="AC16HB_19220"
     CDS             1762..3279
                     /locus_tag="AC16HB_19220"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5608976.1"
                     /translation="MTSSQNSRTLPFATMPPPGRVIARPAFHDPGRWFSHAWDVWLGG
                     WLSPQELQRRSARRLRDLVAYARAHVPLYRELYRDLPRGVALAQLPVVCKRDLMRDLE
                     ASLGDRSIRLPELLRFLERSDAVGRPFMGRYSVWTTSGTTGTPGIFLHDTDALAVYEA
                     LQMFRFRRISSPAELALRFASGERFAMVAATGGHFAGVASIEHLRRTYPWLAPTLRSF
                     SLLQPWPVLVTQLNAYRPTLLATYPTAAEVLADEQDAGRLNLELRELWTGGECLCPNT
                     RRRLEASFGCPVRNEYGASEFMSIAWDCGHGSLHVNSDWVLLEPVDAQRRPVAPGEPS
                     HSVLLTNLANRVQPLIRYDLGDSITVRPGPCACGNRLPAIQVEGRHDDTLAFVDAAGE
                     PVKLLPLVLATVLEEDAHVNDFQLVQTAARRLLLRLGLGERARAEAARAALRSYLQSV
                     GLVSVALQIDTLPPQRSPDSGKLRRVIRSAGVETGRCREEAVSHRPARRPARSAQ"
     gene            3354..>4211
                     /locus_tag="AC16HB_19225"
     CDS             3354..>4211
                     /locus_tag="AC16HB_19225"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR5608977.1"
                     /translation="MLATAAGCSSIGYYAQAARGQFSLLAAARPLDELLADPATPESL
                     KERLRRAREIRAFAARELGLPDNGSYTAYADLGRPAVVWNVFATPTLSLELKTWCYPL
                     FGCAGYRGYFDKGEAERLAGELRAQGWDVNVAPVPAYSTLGWFDDPLLNTFINAQDGE
                     LARMLFHELAHQVVYVRDDTVFNESFATAVERTGVARWLSARHDAVLSQGYAEIDSRR
                     RDFLALLLKYRARLQHVYAEAAARATLLERKREAFEALQAEYRQLRDGRWGGFRGYDR
                     YFAQDLNNAH"
BASE COUNT          587 a         1483 c         1438 g          703 t
ORIGIN      
        1 ccgtggctgg cggagctgaa cgcgcgctgc tcgctctcgt gtgtgcccgc gaggatgtac
       61 gagccggtgc ccatgctgcc gccgatgatc accggctgcc cgacatcgcg gtacggcagc
      121 ggaaccgcgg gatgccccgg gccgaacgcg cgcgtggcgc ccttgcggtg cacgtacagc
      181 cagcggcgct tgcgctcgac ctcatgctgc tccagtttgc aggtgttgtg cgacacgtcg
      241 aacagcgtgt cgacgcttgc ctgcgggaag aagtgcgcaa gcgtgcgccg cgcgatatgc
      301 gcgaggatct ggcggttggc gagcgcgaca ttgatcgccg cgttcattgc gccgaggtac
      361 tcctgcccct cgggcgagtt gatcggcgcg cacgcgagct cccgatccgg cagatggatg
      421 ccgagccggc ttgccgcctt ggcgaggctc accaggtagt cggtgccgat ctggtgcccc
      481 agtccgcgtg aaccgcagtg gatcgacacg acgatctgtc cttcccgcac gccgaacgcc
      541 tgcgcggcct tctcgtcgtg gattctttcg accacctgca cttcgaggta atggttgccc
      601 gagccgagcg tgcccatctc cccgcgctgg cgcttcttcg cggtgttcga cacgctgtcg
      661 ggcactgcgc cccggatgcg gccgcgttcc tcgacgaact ccagctcggc gccggtgccg
      721 tagccgtgcc gcacggccca ctcggcgccc tcgatcatga tgtcgtcgag atcggcatcg
      781 tcgagctgca ggtcgccttc gacgccgacg ccggccggga tgtcgcggaa caggctgtcg
      841 ccgagctttc cgctcttcgg cagcaggtcg tccagcgtga gcgaggtccg caggcaacgg
      901 atgccgcacg agatatcgaa gccgacgccg ccggcagaga tgatgccgcc gcggtcggga
      961 tcgaatgccg ccacgccgcc gatcggaaag ccgtaacccc agtgcgcatc cggcatcgcc
     1021 atcgccgggc cgaccagccc cggcagttgc gcgacgttgc cgatctgctc cagcaccttg
     1081 tcgtccatcg cggcaaccag cgcgcggtcg ccatacagca gcgcggtgcc ctgggtttcc
     1141 gcaggcagtt tcacgcgcca gcagaagtcg tcgatgcgtt ccagtcggtt cagatccatg
     1201 gctgcctcac acgtccacca cgcagcgcgc ctcccagcct gcggcggatg gccgcaccgc
     1261 gagtgccgtc aacgtcgcac ccttgacttc ggtgccgcgc tcctcgtccg gctgccagcg
     1321 ccgaccgcgc gcctcgccgc gccagtgcgc gccgtcgcgc acgacgtgga actcgaagaa
     1381 gacgccgcgc tgctcgcggg ccgcggcgag cagcgcgttg agccaacgca ccagcgccag
     1441 ttcgacatcg tcctcggtga tttcgatctg cgcggtgatc gctgcagcgg gttctgccgc
     1501 gctgcgttcc gacatgatcg cgaacatcgc gcgcgcggca gcctcgaacg cgcgctccat
     1561 gctcgcaccg cggccgatca cgccgatgtc ggcggcgtgc tcgaagtagt ccgtgctgtc
     1621 gttcgttgcg ctcacggttc cgtcaaccta agtggacgac cgattcacga gttgaccttt
     1681 cgcaatgcgt caaccgaacc gtgtcgcacg cttgcggcat gggcaggtcg cacgcagcgc
     1741 gaccacaatg gcgtcctccg catgacttcc tcgcagaact cccgcacgct gcccttcgcg
     1801 acgatgccgc cgcccggacg ggtgattgcg cgccccgcct ttcacgatcc gggccgctgg
     1861 ttttcgcacg catgggacgt ctggctcggc ggctggctgt caccgcagga actgcagcgg
     1921 cgcagcgcac ggcgcctgcg cgatctggtg gcgtacgcgc gcgcgcacgt gccgctgtat
     1981 cgggaactgt atcgcgacct gccgcgcggc gtggcactcg cgcaattgcc ggtcgtgtgc
     2041 aagcgcgacc tgatgcgcga tctcgaggcg agcctcggcg atcgatcgat ccgcttgccc
     2101 gaactgctgc gtttcctcga gcgcagcgac gccgtcggcc gcccgttcat ggggcgttac
     2161 tcggtgtgga ccacgtccgg cacgaccggc acgcccggca tcttcctgca cgacaccgac
     2221 gcgctggcgg tgtacgaggc actgcagatg ttccgcttcc ggcgcatctc ctcgccggcc
     2281 gaactggccc tgcgcttcgc gtccggcgag cggtttgcga tggtcgcggc cacgggcggg
     2341 cacttcgccg gcgtggcgag catcgagcac ctgcgccgga cctatccgtg gctggcgccg
     2401 acgttgcggt cgttctcgct gctgcagccg tggccggtgc tggtcacgca gttgaacgcg
     2461 tatcggccga cgctgctggc gacgtatcca acggctgccg aagtgcttgc cgacgagcag
     2521 gatgcgggcc ggctgaatct ggaactgcgc gaactgtgga ccggcggcga atgcctgtgc
     2581 ccgaatacgc gtcggcggct cgaggcgagc ttcggctgcc cggtgcgcaa cgagtacggt
     2641 gcctccgagt tcatgtcgat cgcgtgggac tgcggccacg gcagcctgca cgtgaacagc
     2701 gactgggtgc tgctggagcc ggtcgacgcg cagcggcggc cggtcgcacc gggcgagccg
     2761 tcgcattccg tgctgctgac caaccttgcc aaccgcgtgc agccattgat ccgctacgac
     2821 ctcggcgatt cgatcaccgt gcggcccggt ccatgcgcgt gcggcaatcg cctgccggcg
     2881 atccaggtcg aagggcggca cgacgacacg ctggcattcg tcgacgccgc gggtgaacca
     2941 gtcaagctgc tgccgctcgt gctggcgacc gtgctggagg aggacgcgca cgtcaacgac
     3001 ttccagctcg tgcagaccgc ggcgcgccgg ctgctgctgc ggttggggct cggtgaacgc
     3061 gcacgtgccg aagcggcgcg cgccgcgctg cgcagctacc tgcaatcggt cggtctcgtc
     3121 tccgtcgcgc tgcagatcga cacgctgcca ccgcagcgca gtcccgacag cggcaagctg
     3181 cgccgtgtga ttcgcagcgc cggcgtcgag accgggcgct gccgcgaaga ggctgtgtcg
     3241 catcggcctg cacgccgtcc cgcgcgctcg gcacaatagc cggcgtgcgc aagcggcttc
     3301 gtctttcact tcgttccggt cgatggctgc gctggggtgt ccctgcgctt ctggtgctgg
     3361 ccactgccgc cggctgttcg tcgatcggct actacgcgca ggcagcgcgc ggccaatttt
     3421 cgctgctcgc ggccgcccgc ccgctcgacg aactgctcgc cgatccggcc acgcccgagt
     3481 cgctgaagga gcggctgcgg cgcgcgcgcg agatccgcgc cttcgccgcg cgcgagctgg
     3541 gcctgcccga caacggcagc tacaccgcgt acgcggacct cggccggccg gcggtggtgt
     3601 ggaacgtctt cgcgacgccg accctgtcgc tggaactgaa gacctggtgc tatccgctgt
     3661 tcggctgcgc cggctatcgc ggctacttcg acaagggcga agcggagcgc ctcgccggcg
     3721 aactgcgcgc gcagggctgg gacgtcaacg tcgcgccggt gccggcctac tcgacgctgg
     3781 gctggttcga cgatccgctg ctgaacacgt tcatcaatgc gcaggatggc gagctggcgc
     3841 gcatgctgtt ccacgaactg gcgcaccagg tcgtgtacgt gcgcgacgac acggtgttca
     3901 acgagagctt tgccacggcg gtcgagcgca ccggggtggc gcgctggctt tcggcgcgcc
     3961 atgacgcggt gctgtcgcag ggctacgccg agatcgattc gcgccggcgc gattttctcg
     4021 cgctgctgct caagtaccgc gcgcggctgc agcacgtcta tgccgaggcg gcagcgcgcg
     4081 ccaccctgct cgaacgcaag cgcgaggcgt tcgaggcatt gcaggcggag taccggcagc
     4141 tgcgcgacgg ccgctggggc ggcttccgcg ggtacgaccg ctatttcgcg caggacctga
     4201 acaacgcgca c
//
LOCUS       DBNHBY010000826         4350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1691537, whole genome shotgun sequence.
ACCESSION   DBNHBY010000826 DBNHBY010000000
VERSION     DBNHBY010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1691537"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="AC16HB_19230"
     CDS             complement(<1..1238)
                     /locus_tag="AC16HB_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5608978.1"
                     /translation="MKVRKELWFGFSLMAVIVVTVVIFTPWANFIDGHLSRDDLGHLG
                     LLMLALIVVAIMLGFPTAFTLMGMGVFFAWLAYRSVNPDLAVRQVLDLMVQRAYSVMS
                     NDVLIAIPLFVFMGYLVERAALIDRLFKSLHLALARVPGSLAVATIVTCAIFATATGI
                     VGAVVTLMGLLAYPAMLRAGYDVKVSAGAITAGGCLGILIPPSVLLIVYGATAGVSVV
                     QLYAGAFFPGLMLAGLYVGYVIVLAKFKPRLMPPLSDAERHVVLPAFAQTLSARGANA
                     ISGLLRAASGNAGVPSAIAVKQLFVSLLPMLTIAATLAFTYQAATAPAVVESPAGLVQ
                     AGGEISADIKEEPAGLVGGLAEPKEEDTGLQEPPGVGAEEKKEAAPEPAKEEKAAEMT
                     PAEQPVAERLPVPVWFWAVLA"
     gene            complement(1235..1858)
                     /locus_tag="AC16HB_19235"
     CDS             complement(1235..1858)
                     /locus_tag="AC16HB_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR5608979.1"
                     /translation="MQKLLLTIDKISTFIGQSFSWLIVSLTLMISWEVFSRYVLDHPH
                     SWAFDVMIIMYGTLFMMAGAYTLAKAGHVRGDVLYGFFRPRTQATIDLILFIIFFIPG
                     VIALTYAGYYYAAESWAINEHSNITAEGPPIYPFKTVIPLAGAFLLVQGIVEIIRCVI
                     CIKQGEWPSREEDVEEVDVEKLKEMVHVKDEDIAKLDELVVHQEQTK"
     gene            complement(1928..3067)
                     /locus_tag="AC16HB_19240"
     CDS             complement(1928..3067)
                     /locus_tag="AC16HB_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5608980.1"
                     /translation="MDFIKPKAATRRNFIARAAAGSAVLGVPMIAKGQAGPISMRWQS
                     TWPSKDIFHEYALDYAKKVNDMTGGELKIEVLPAGAVVPAFGLLDAVSKGTLDGGHGV
                     LGYNYGKQNALALWNSGPAFGMDANMLLAWHKYGGGQELLTKLYASIGGNVVSFLYGP
                     MATQPLGWFKKPITKTADFKGLKFRTNGLAIDLFTAMGAAVNALPGGEIVPAMDRGLL
                     DGAEFNNATSDRILGFPDVSKVCMLQSYHQSAETFEITFNKPKYEALPAKLKAVIANA
                     VEAASADMSWKAIDRYSKDYIELQTKDKVKFYKTPDSVLQDQLTLWDAIVERKSAENP
                     LFKEIAASQKAFAERAVKWDQDTYVSRRMAVNHFFGAKKAEAKKA"
     gene            3318..3605
                     /locus_tag="AC16HB_19245"
     CDS             3318..3605
                     /locus_tag="AC16HB_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5608981.1"
                     /translation="MKLRLLAASALTLATLMSAAPAYAQGPMEFKWDDFLKMADVNKD
                     GMVTKKEFLDAMGKTWDKGMAKMKSDAKMVKGDMMTMDGVKALLADLYRGA"
     gene            complement(3619..>4350)
                     /locus_tag="AC16HB_19250"
     CDS             complement(3619..>4350)
                     /locus_tag="AC16HB_19250"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR5608982.1"
                     /translation="NGDGFGVGWYAARTEPGLYRDYRPAWNDENLRSLAEQIQAHLFF
                     AHVRASTGGLTSRANCHPFRCGRWLFMHNGQIGGFDAIARELDFALAPALYRARQGTT
                     DSETLFYLALTFGLQDEPLAALARAAGFVEEVMQRNGIADPLRLTAALTDGQRIFAVR
                     YSSDRASPSLYFGGASAVRDSEGRRCVDGDDESLLVVSEPLDRAFDNWTAVPEGHAVM
                     AEPGGVLVVPFEPRAGVRSASTAVA"
BASE COUNT          859 a         1404 c         1325 g          762 t
ORIGIN      
        1 gcgagcacgg cccagaacca gaccggcacg ggcagccgtt cggcaacggg ctgctcggcc
       61 ggagtcattt cagccgcctt ctcctctttc gccggttcgg gtgcggcttc cttcttttcc
      121 tccgcgccga cgccgggagg ctcctgcagt ccggtgtcct cttcctttgg ctccgccagt
      181 ccaccgacta gacccgccgg ctcctccttg atgtcggccg aaatctcacc gccggcctgc
      241 accagccctg cggggctctc gaccacggca ggggcggtcg ccgcttgata ggtaaacgcc
      301 agcgtcgccg cgatcgtcag catcggcagt aacgagacga acaactgttt cactgcaata
      361 gccgaaggca cgcctgcgtt gccactcgca gcacgcagca ggccggaaat tgcattggca
      421 ccacgcgccg aaagcgtctg cgcgaaggcc ggaagcacca cgtgacgctc agcgtccgac
      481 aatggcggca tcaatcgcgg cttgaacttc gccagcacga tgacgtagcc aacatagagt
      541 ccagccagca tgagcccggg aaagaaggcg cccgcataca actgcacgac cgagacgccg
      601 gccgtggcgc catagacgat cagcagcacc gacggcggaa tcaggatgcc gaggcagccc
      661 ccggccgtaa tcgcgcccgc cgaaaccttg acgtcgtagc cggcacgcag catcgccgga
      721 tacgcgagca aacccatcag cgtcacgacc gcgccgacga ttccggtggc cgtggcgaaa
      781 atcgcgcagg tgacgatggt cgccaccgcg agtgatccgg gcacgcgagc gagcgccagg
      841 tgcaggctct tgaacaaccg atcgatcagc gccgcacgtt ctaccagata gcccatgaag
      901 acaaacagcg gaatcgcgat cagtacgtcg ttcgacatca ccgagtacgc gcgctgcacc
      961 atcaggtcga gtacctggcg gacggcgaga tcgggattca cgctgcgata ggcaagccag
     1021 gcgaagaaga cgcccatgcc catcagcgtg aaagcggttg ggaagccgag catgatcgcc
     1081 accacgatca gcgccagcat cagcagcccg aggtgcccaa gatcgtcccg gctcagatgc
     1141 ccgtcgatga agttggccca gggtgtgaag atcaccaccg tgacgacgat caccgccatc
     1201 agcgagaagc cgaaccaaag ttccttgcgg accttcattt ggtctgctcc tgatgcacga
     1261 cgagttcatc gagcttggcg atgtcctcat ccttgacgtg caccatctcc ttcagcttct
     1321 cgacatcgac ttcctcgacg tcctcctcgc gcgacggcca ctctccctgc ttgatacaga
     1381 tcacgcagcg aatgatctcg acaatcccct gcaccagcag gaacgcgccg gcgagcggta
     1441 tcacggtctt gaacggataa atgggcgggc cttcggccgt gatgttggag tgctcgttga
     1501 tcgcccacga ttcggccgcg tagtagtagc cggcataagt gagcgcgatc accccaggaa
     1561 tgaagaagat gatgaacaga atcagatcga tcgtcgcctg cgtgcgcggc cgaaagaagc
     1621 cgtacagcac atccccgcgc acatggccgg ccttggccag cgtatacgca ccggccatca
     1681 tgaacagcgt gccatacatg atgatcatga cgtcgaaggc ccaggagtgc ggatggtcca
     1741 acacatagcg cgagaacact tcccaggaga tcatcaaagt aagcgagacg atgagccagg
     1801 aaaacgactg gcctatgaag gtgctgatct tgtcaatcgt aagaagcagc ttctgcattt
     1861 tctggcctcg aaaaaaaatc agccacccgg accttgcccg gatggccgat gaacgatgcg
     1921 aaaaccctca ggccttcttg gcttcagcct tcttggcgcc aaagaaatgg ttcacggcca
     1981 tgcgacggct gacgtaggtg tcctggtccc atttcaccgc gcgctcggcg aatgccttct
     2041 gcgatgccgc gatttccttg aacagggggt tttccgccga tttccgttcc actatcgcgt
     2101 cccacagcgt aagttggtct tgcagcaccg aatccggggt cttgtagaac ttaaccttgt
     2161 ccttggtctg aagttcgatg tagtccttgg aataacgatc gatcgctttc caggacatat
     2221 cggcggacgc cgcttccacg gcattggcga tgaccgcctt caatttcgcc ggcagcgcct
     2281 cgtacttcgg cttgttgaaa gtgatctcga acgtctcggc gctctggtga tagctctgca
     2341 gcatgcacac cttggacacg tcggggaagc cgaggatgcg atcggaggtg gcattgttga
     2401 attcggcacc atcgagcagg ccacggtcca tggccggcac gatctcgccg ccgggaagcg
     2461 cgttgacggc ggcccccatc gcagtgaaca aatcgattgc caggccgttg gtgcggaatt
     2521 tgagtccctt gaaatccgcc gtcttggtga tgggtttctt gaaccagccc agcggctgtg
     2581 tcgccatcgg gccgtagagg aaggacacca cattgccgcc gattgacgcg taaagcttcg
     2641 tcagcagttc ctggccgcca ccatacttgt gccaggcgag caacatgttg gcgtccatgc
     2701 cgaacgccgg gccggaattc cagagtgcca gcgcgttctg cttgccatag ttgtaaccca
     2761 ataccccgtg tccgccgtcc agcgtgccct tggacacggc gtcgagcaat ccgaaggcgg
     2821 gcactacggc acccgcgggc agcacctcga tcttcagctc gccgccggtc atgtcattga
     2881 ccttcttcgc gtagtccagc gcgtactcgt ggaagatgtc tttcgatggc caggtgctct
     2941 gccagcgcat cgagatcggc cccgcctgtc ctttggcgat catcgggacg ccgagcacgg
     3001 ccgagcctgc cgccgcgcgg gcaatgaaat tgcggcgcgt ggccgccttg ggcttgatga
     3061 aatccatgaa tagtctccag tcggttggtc tcaaatcagc ccacgttcgt gggctcccca
     3121 cttgtattgg ctcgcggcgc ctgcgggttg ggccttgatg ccgcgtttgc ggccattcta
     3181 gaagaacccc cggccagcgt cagccggata tttcctgtaa gtcccggcag gcatccgcga
     3241 ccaatcgccg gagccgcgtc gtcgctgtgc aatgtccggc gcgcgggtca caaccaccaa
     3301 ccccgaagga gaagacgatg aaactacgac tgctggccgc ctctgctctc accctcgcga
     3361 cgctgatgtc cgccgcgccc gcctatgcgc aaggtccgat ggaattcaag tgggacgact
     3421 tcctgaagat ggccgacgtg aacaaggacg gcatggtgac gaagaaggag ttcctcgacg
     3481 ccatgggcaa gacctgggac aagggcatgg cgaagatgaa gagcgacgcc aagatggtca
     3541 agggcgacat gatgacgatg gacggcgtca aggcgctgct ggccgatctg tacaggggcg
     3601 cgtgactcgc agttctgttc acgccaccgc ggtcgatgct gaacggaccc cggcgcgggg
     3661 ctcgaacggc accaccagca cgccgccggg ctcggccatc accgcgtgtc cctcgggcac
     3721 cgcggtccag ttgtcgaacg cgcggtcgag cggctccgag acgacgagca gcgactcatc
     3781 gtcgccgtcg acacagcggc gcccctcgct gtcgcgcacg gcgctggcgc caccgaagta
     3841 cagcgacggc gaggcgcggt ccgacgaata gcggaccgcg aagatccgct ggccgtcggt
     3901 gagcgccgcg gtcaggcgca gcggatcggc gatgccgttg cgttgcatga cttcctcgac
     3961 gaatcccgcg gcgcgcgcca gcgctgccag cggttcatcc tgcagtccga aggtgagcgc
     4021 cagatagaac agcgtttcgc tgtcggtcgt cccctggcgc gcgcgataca gggccggggc
     4081 gagcgcgaaa tccagctcgc gcgcgatcgc gtcgaagccg ccgatctggc cgttgtgcat
     4141 gaacagccat cgcccgcacc ggaacggatg gcagttggcg cggctggtca gaccgccggt
     4201 ggacgcgcgc acgtgcgcga agaacaggtg cgcctggatc tgctcggcca gactgcgcag
     4261 gttctcgtcg ttccacgccg gccggtagtc gcgatacaga ccgggctccg tccgcgccgc
     4321 gtaccagccg acgccgaagc cgtcgccgtt
//
LOCUS       DBNHBY010000827         4327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1487121, whole genome shotgun sequence.
ACCESSION   DBNHBY010000827 DBNHBY010000000
VERSION     DBNHBY010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1487121"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..974)
                     /gene="tolA"
                     /locus_tag="AC16HB_19255"
     CDS             complement(186..974)
                     /gene="tolA"
                     /locus_tag="AC16HB_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR5608983.1"
                     /translation="MTAAAITSPRAPRPTENTTGAFVLALAMHALLFAALWINVQWRT
                     QPQGEVTAELWGALPPPVEVAPPPPPPPVVERKEPEQRDADIVLQQERRKRLEEEKRR
                     EEEQKKREAEQRKLDEAKRKADEAKRKADDEKRAAAQREAIRKAEEQRMLAQAGAGAP
                     TSGTTGGRRGDASYNALLQSLIKPRIVFAVPDGTTRKIFATVQVDLLPTGEIVAVKVL
                     KPSGLPGYDAAVERAIRRTDPFPRKPDGTIERTITIDFYPVDAQ"
     gene            complement(971..1405)
                     /locus_tag="AC16HB_19260"
     CDS             complement(971..1405)
                     /locus_tag="AC16HB_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5608984.1"
                     /translation="MALLLRRKDGGRRRLLADINVVPYIDVMLVLVVILMVSAPFVNP
                     SLVNLPRVGKASRSPDRPLEIVIKVDGKLLLRDTGKELANDLPGAVAIIKQRQKAKPD
                     SPTPVVIAADKDVRYEQVMKVMSALQKENVARVGLSVQPEPK"
     gene            complement(1487..2158)
                     /gene="tolQ"
                     /locus_tag="AC16HB_19265"
     CDS             complement(1487..2158)
                     /gene="tolQ"
                     /locus_tag="AC16HB_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR5608985.1"
                     /translation="MNASADLSILSLVTKATLVVQLVMALLLIVSLASWTVIFGKAFS
                     IRRARKETDAFEREFWSGTDLNTLYQRATNRRRRTCALERIFESGMSEFHKLHGRGDA
                     QATLDGARRAMRASYQREMDALEGRLQFLASVGSVSPYIGLFGTVWGIMHAFTGLASL
                     EQATLASVAPGIAESLVATAIGLFAAIPAVVAYNRFAHDIDRLANRFETFIEEFSNIL
                     QRHRQ"
     gene            complement(2155..2583)
                     /gene="ybgC"
                     /locus_tag="AC16HB_19270"
     CDS             complement(2155..2583)
                     /gene="ybgC"
                     /locus_tag="AC16HB_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR5608986.1"
                     /translation="MVATTLPHFDWPVRVYFEDTDAGGIVYYANFLKFFERCRTEWLR
                     ALGVEQQQLRSALGLQFVVVKASIDYRRPARLDDWLTIRARIAELGRTYLVFAQQALR
                     ADELLATAEVKVACLDAQSMAPARLPASLATKIKTESLPR"
     gene            2729..>4327
                     /locus_tag="AC16HB_19275"
     CDS             2729..>4327
                     /locus_tag="AC16HB_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608987.1"
                     /translation="MGLAALGSGRAAALAALLPLLTAIGCSEVVNNPHPRGSEKTNTL
                     FTAFQERPKYLDPVSSYNNNETPWVFNIYEPPLRYHYLKRPYTLEGNTAIALPDVRYL
                     DKDGKPLPDDAPAERIALSIYTLKLKPGIKFQPHPAFARRADGSYLYQNLAPADIAGK
                     FEIADFPLSDAAVTTRELTADDYVYQIKRLASPYVPTPSPLYGLLNQYIVDLKGLGDR
                     LRAEHKQALAGRNPQDLYLPLRDLRYARFECARAIDARTLEIRVLGKYPQFKYWLAMP
                     FFSPVPWEADRFYAQRGMADHALTLNQWPVGSGPFMLVEQGPNRYVMERNPNFRGEPY
                     PCEGAPGDRERGWLDDCGKTTPFVDRMVSTIEKERQPLEAKVIQGYYDVPQIERLDDG
                     FKLQQELRDQTGRWKMLQDHGIQLPTSVEPNSWYMGFNWLDPVVGRGSTPEEEVRHRK
                     LRQAISIATDWEEYTNIFFDTYGEAETAMSPLPPGVFGYRAGCDGIDPVTHVCRDGRP
                     QRRSLDEAKKLLAEAGYPGGRDART"
BASE COUNT          713 a         1467 c         1434 g          713 t
ORIGIN      
        1 ctggaaattc gcgatcgcga tcgggaactg gttggagccg acgccggtga tctcgacccg
       61 cagctgcgcg gcagccagcg agcaccaggc gagtacggca caggcggccg cgcgcatcag
      121 ggtcttcgac atgtgcgtca aattataaga agccatgctc tcgggatgat cgaacggccg
      181 tcacgtcatt gcgcgtcaac cggatagaaa tcgatcgtga tggtgcgttc gatcgtgccg
      241 tcgggcttgc ggggaaacgg atcagttcgg cggatcgcgc gctcgaccgc tgcgtcatag
      301 cccggcaacc cgctcggctt cagcaccttc acggcgacaa tttcgccggt gggcagcaga
      361 tcgacttgaa cggttgcaaa gatcttacgt gtcgtcccgt ctggcaccgc aaagacgatg
      421 cgaggcttga ttagcgattg aagcagcgcg ttgtagcttg catcgcccct gcggccgccg
      481 gtcgtgcccg acgtcggtgc accggcaccg gcctgcgcga gcatccgctg ttcctcggcc
      541 ttgcggatcg cttcgcgctg ggcggcggcg cgcttctcat cgtccgcctt gcgcttcgct
      601 tcgtcggcct tgcgcttggc ttcgtcgagc ttgcgctgct cggcctcgcg cttcttctgc
      661 tcttcctcgc gtcgcttctc ttcttccagc cgcttcctgc gctcctgttg cagcacgatg
      721 tcggcatcgc gctgctcggg ctccttgcgt tcgaccaccg gcggcggcgg gggcggcggt
      781 gcgacctcga ccggtggcgg cagcgcgccc cacagttcgg cggtcacctc gccctgcggc
      841 tgcgtgcgcc actgcacgtt gatccacaac gcggcgaaca gcagcgcgtg catcgccagc
      901 gccagcacga acgcgccggt cgtgttctcg gtcggtcgcg gcgcgcgcgg cgaggtgatc
      961 gctgctgcgg tcatttcggt tcgggttgca ccgacaggcc gacgcgcgcg acgttctcct
     1021 tctgcagcgc gctcatcacc ttcatcacct gctcgtagcg cacgtccttg tcagccgcaa
     1081 taacgacggg cgtcggcgaa tcgggtttgg ccttctgccg ctgcttgatg atcgccaccg
     1141 cgccgggcag gtcgttcgcc agttccttgc cggtatcgcg cagcagcagc ttgccgtcga
     1201 ccttgatcac gatttccagc ggccggtccg gcgacctcga cgccttgccc acacgcggca
     1261 ggttgacgag gctcggattc acgaacggcg ccgacaccat cagtatcact acgagcacca
     1321 gcatcacgtc gatgtacggc acgacgttga tgtcggccag caggcgcctg cgcccgccgt
     1381 ccttgcgccg aagcagcagc gccatctcag cgcccgccgg gccgccccag gggcgctgag
     1441 cgcccccgcg cggggcagcg aacgaagtga gcgtggcggt ccacgatcat tgccggtgac
     1501 gctgcaggat gttcgagaac tcctcgatga aggtctcgaa ccggttggcc agccgatcga
     1561 tgtcgtgcgc gaagcggttg tacgcgacga cggccgggat cgccgcgaac aggccgattg
     1621 ccgtggccac cagcgactcg gcgatgccgg gcgccacgct cgccagcgtg gcctgctcga
     1681 ggctcgccag cccggtgaag gcgtgcatga tgccccacac ggtgccgaac aggccgatgt
     1741 aggggctgac cgagcccacc gaagcgagga actgcagccg gccttcgagc gcgtccatct
     1801 cgcgctggta gctggcgcgc atcgcgcgcc gcgcgccgtc cagcgtcgcc tgcgcatcgc
     1861 cgcggccgtg cagcttgtgg aactcgctca tgcccgactc gaagatgcgt tccagcgcgc
     1921 aggtgcggcg gcgccggttg gtggcccgct ggtacagggt gttcaggtcc gtcccgctcc
     1981 agaactcacg ttcgaaggca tcggtctcct tgcgtgcgcg ccggatcgag aacgccttgc
     2041 cgaagatcac ggtccacgac gcgagcgaaa cgatcagcag cagcgccatc accagctgca
     2101 ccaccagcgt ggccttggtc accagcgaca gaatcgacag gtcggcggaa gcgttcatct
     2161 cggcaggctt tcggtcttta tctttgtcgc cagcgaggcg ggcaggcgtg ccggcgccat
     2221 cgactgcgcg tccaggcatg ccaccttgac ctccgcggtg gccaggagtt catcggcgcg
     2281 cagcgcctgc tgcgcgaaga cgagatacgt gcgccccaat tctgcaatcc gggcgcggat
     2341 cgtgagccaa tcgtccagcc tagcgggccg gcggtagtcg atcgacgcct tcaccaccac
     2401 gaactgcagg ccgagggcgg agcgcagctg ctgctgctcg actccgagcg cgcgcaacca
     2461 ttcggtgcgg cagcgttcga agaacttgag gaaattggcg tagtagacga tgccgccggc
     2521 gtcggtgtct tcgaagtaca cgcggaccgg ccagtcgaag tgaggcagcg ttgtggcgac
     2581 cacggacgcc gagtctatcg cggcagggcg gcgttcgtcg cagcgtgcgg ctcccgccga
     2641 gtggcaacgt acaatccgcg cccttgtaca agaacgaccg cgcgacggtt ccccctcacg
     2701 aggggcgggg cgaaggagaa tcgcgacgat ggggctggca gctttgggtt cggggcgcgc
     2761 ggccgcgctg gcggcgctgc tgccgctgct cacggcgatc ggctgcagcg aggtggtcaa
     2821 caatcctcat ccgcgcgggt ccgagaagac caacacgctg ttcaccgcgt tccaggagcg
     2881 gccgaagtac ctcgatccgg tcagctcgta caacaacaac gagacgccgt gggtcttcaa
     2941 catctacgag ccgccgctgc gctaccacta cctgaagcgg ccgtacacgc tggaaggcaa
     3001 caccgcgatc gcgctgcccg acgtccgcta tctggacaag gacggcaagc cgctgccgga
     3061 cgatgcgccc gcagaacgga tcgcgctctc gatctacacg ctgaagctca agcccggcat
     3121 caagttccag ccgcacccgg ccttcgcgcg gcgggccgac ggcagctatc tgtatcagaa
     3181 cctggcgccc gcggacatcg caggcaagtt cgagatcgcc gactttccgc tgtcggacgc
     3241 cgccgtcacg acgcgcgaat tgaccgctga cgactacgtg taccagatca agcggctcgc
     3301 cagtccctac gtgcccacgc cgtcgccgct gtacgggttg ctgaaccagt acatcgtcga
     3361 cctgaagggg ctcggtgacc ggctgcgtgc cgagcacaag caggcgctcg ccggccgcaa
     3421 tccgcaggac ctgtacctgc ctttgcgcga cctgcgctac gcgcgcttcg agtgcgcgcg
     3481 cgcgatcgac gcgcgcacgc tcgagatccg cgtgctgggc aagtacccgc agttcaagta
     3541 ctggctggcg atgccgttct tttcgcccgt gccgtgggag gccgaccggt tctacgctca
     3601 gcgcggcatg gccgaccacg cgctgacgct gaaccagtgg ccggtcggca gcgggccgtt
     3661 catgctggtc gagcaggggc cgaaccgcta cgtgatggaa cgcaatccca atttccgggg
     3721 cgagccgtat ccgtgcgaag gcgctcccgg cgacagggag cggggctggc tcgacgactg
     3781 cggcaagacg acgcccttcg tcgatcggat ggtctcgacg atcgaaaagg agcgccagcc
     3841 gctcgaggcc aaggtgatcc agggctatta cgacgtgccg cagatcgagc gcctcgacga
     3901 cgggttcaag ctgcagcagg aactgcgcga ccagaccggc cgctggaaga tgctgcagga
     3961 tcatggcatc cagttaccga cgtcggtcga gccgaacagc tggtacatgg gcttcaactg
     4021 gctcgacccg gtggtcggcc gcggctccac gccggaggaa gaggtgcgcc atcgcaagct
     4081 gcgccaggcg atctcgatcg cgaccgactg ggaggaatac accaacatct tcttcgacac
     4141 ctacggcgag gccgagaccg cgatgagccc gctgccgccg ggcgtgttcg gctaccgcgc
     4201 cggctgtgac ggcatcgatc cggtcacgca tgtctgcagg gacggccggc cgcagcgccg
     4261 ctcgctcgac gaggcgaaga agctgctggc cgaggccggc taccccggcg gccgcgacgc
     4321 aaggaca
//
LOCUS       DBNHBY010000828         2612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_540524, whole genome shotgun sequence.
ACCESSION   DBNHBY010000828 DBNHBY010000000
VERSION     DBNHBY010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_540524"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="AC16HB_19280"
     CDS             <1..465
                     /locus_tag="AC16HB_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5608988.1"
                     /translation="SEFDRVLGLELGADDYLAKPFSVIELMARVKAIFRRVDALAKPS
                     AQAPKTIVAGELAIDIDRRHVTLAGRTIELTAKEFDLLVFFAAHPGRVFNRGQLLDKV
                     WGYSHSGYEHTVNSHINRLRAKIEADPANPRYILTVWSVGYKFAEVPAPSGQ"
     gene            complement(459..1118)
                     /locus_tag="AC16HB_19285"
     CDS             complement(459..1118)
                     /locus_tag="AC16HB_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5608989.1"
                     /translation="MAQQLAATLDGLDEDGGGATASEFLGRTGGALAQIDPGELEQHR
                     AQLMRYALWLLGSRAAAEDAVQDTLLAALQAPANFAGRSSIRTWLFGILKHKIADTFR
                     RQSRELPLEDDDDDGANEPRALFTEDGQWREPPSDWGDPEAALSQRRFFDVLERCIDR
                     LPKNAARVFTMREILGMETPEICAAVGITANNCFVMLHRARTTLRTLLEQDWFDPRPA
                     H"
     gene            1257..2441
                     /gene="pncB"
                     /locus_tag="AC16HB_19290"
     CDS             1257..2441
                     /gene="pncB"
                     /locus_tag="AC16HB_19290"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR5608990.1"
                     /translation="MIITSLLDTDLYKFTMMQVVLHQFPGAQVEYRFKCRNAGVDLTP
                     YIDEIREQIHWLCTLRFKEPELEYLRGMRFIKSDFVDFLGLFQLNAKYITVRPSPLAN
                     GEIEIVIKGPWLHTILFEIPVLAIVNEVYFRATQPEPDFAEGRRRLTRKIALIRDDAS
                     LAGPGGPKIADYGTRRRFSRRWHDEVLHTLKGELRDNLAGTSNVMFAFRHGLIPLGTM
                     AHEYLQACQALGPRLRDSQTFGFEMWAKEYRGDLGIALSDVFGMDAFLRDYDMYFCKL
                     FDGTRHDSGDALDWGERMIAQYEKHRVDPKTKTLVFSDALTIPKVIELFRRFNGRARL
                     AFGVGTNLTNDLGYTPLQIVIKMVRCNGQPVAKLSDSPEKNMCDDAAYLAYLRQVFEI
                     RS"
BASE COUNT          442 a          876 c          862 g          432 t
ORIGIN      
        1 tcggagttcg accgcgtgct cggactcgaa ctcggcgccg acgactatct ggcgaagccg
       61 ttttccgtga tcgagctgat ggcgcgcgtg aaggcgatct tccgccgggt cgacgcgctg
      121 gcgaagccgt cggcgcaggc accgaagacg atcgtcgccg gtgaattggc gatcgacatc
      181 gaccggcgcc acgtgacgct ggccggccgc accatcgagc tgaccgccaa ggaattcgat
      241 ctgctggtgt tcttcgccgc ccatccggga cgcgtgttca accgcggcca gctgctcgac
      301 aaggtgtggg gctattccca cagcggctac gagcacaccg tcaattcgca catcaaccgg
      361 ctgcgcgcca agatcgaggc cgatccagcc aacccccgct acatcctgac cgtgtggagc
      421 gtgggctaca agttcgccga ggttccggcg ccgagcggtc agtgagccgg acgcgggtcg
      481 aaccagtcct gctcgagcag cgtgcgcagc gtcgtgcgtg cccggtgcag catcacgaag
      541 cagttgttcg ccgtgatgcc gacggcggcg cagatttccg gagtctccat cccgaggatc
      601 tcgcgcatcg tgaatacgcg cgccgcgttc ttcggcaggc ggtcgatgca gcgctcgagc
      661 acatcgaaga aacgacgctg ggacaaggcc gcttccggat cgccccagtc cgatgggggc
      721 tcgcgccact ggccgtcctc ggtgaagagc gcgcgcggct cattcgcgcc gtcgtcgtcg
      781 tcgtcttcca gcggcagctc gcgcgactgg cggcggaacg tgtcggcgat cttgtgcttc
      841 aggatcccga acagccacgt gcggatggag gagcggcccg cgaagttggc cggtgcctgc
      901 agggcggcca gcaacgtgtc ctgcacggcg tcctcggccg cggcgcggct gccgagcagc
      961 cacagcgcgt agcgcatcag ttgcgcgcga tgctgctcca gttcgccggg gtcgatctgc
     1021 gccagcgcgc cgccggtgcg gccgaggaat tcgctcgccg tcgccccgcc gccgtcctcg
     1081 tccaggccgt cgagcgtcgc tgcaagctgc tgagccatgg tgtcctccgc gttctcgttg
     1141 cagcgaagga tgcttgccgc gcctcacccg gcattcacgg aagcttcaaa ttttcgtcac
     1201 ggccgccggc ggccagcccc taaaatgggg gcggagaccc cgccgatggg ccgcgcgtga
     1261 tcatcaccag cctgctcgac accgacctgt acaagttcac gatgatgcag gtcgtgctgc
     1321 accagtttcc cggtgcccag gtcgaatacc gcttcaagtg ccgcaatgcc ggcgtcgacc
     1381 tgacgccgta tatcgacgag atccgcgagc agatccactg gttgtgcacg ctgcgcttca
     1441 aggagcccga gctcgagtac ctgcgcggca tgcgcttcat caagagcgac ttcgtcgact
     1501 tcctcggcct gttccagctc aacgcgaaat acatcacggt gcggccgtcg ccgctggcca
     1561 acggcgaaat cgagatcgtg atcaaggggc cctggctgca cacgatcctg ttcgagatcc
     1621 cggtgctggc gatcgtcaac gaggtctact tccgcgccac ccagcccgag cccgacttcg
     1681 ccgagggtcg gcgccggctc acgcggaaga tcgcattgat ccgggacgat gcgagcctcg
     1741 cgggacccgg cggcccgaag atcgccgact acggcacgcg ccgccgtttc tcgcgacggt
     1801 ggcacgacga ggtgctgcac acgctgaagg gcgagctgcg cgacaacctg gccggcacca
     1861 gcaacgtgat gttcgcgttc aggcacgggc tgattcctct cggtacgatg gcgcacgagt
     1921 acctgcaggc ctgccaggcg ctcgggccgc ggctgcgcga ctcgcagacc ttcggcttcg
     1981 agatgtgggc caaggagtat cgcggcgacc tcggcatcgc gctgtcggac gtgttcggca
     2041 tggacgcgtt cctgcgcgac tacgacatgt acttctgcaa gctcttcgac ggtacgcgtc
     2101 acgactcggg cgatgcgctc gactggggcg agcgcatgat cgcgcagtac gagaagcacc
     2161 gcgtcgaccc gaagaccaag acgctggtgt tctccgacgc gctgacgatt cccaaggtga
     2221 tcgaactgtt ccggcgcttc aacgggcgcg cgcggctggc gttcggcgtg ggtaccaatc
     2281 tgacgaacga cctcggctac acacctctgc agatcgtgat caagatggtc cgctgcaacg
     2341 gtcagccggt ggcaaaactg tcggattctc ccgagaagaa catgtgcgac gacgcggcgt
     2401 acctcgcgta cctgcggcag gtcttcgaaa tcaggagctg agatcggatc catggtcgag
     2461 aacgccaacg aagcgcgcca gcgcgcggca gtcgtcaagg gcgccaacca gctggtcaac
     2521 gaggccgcgg ccgagccgct gatcgcggcg gtcgagcagg gcgagatgag cgcggccgtc
     2581 ccgaccgcgc gcttcgacct cgtgcccgcg gc
//
LOCUS       DBNHBY010000829         2576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_202319, whole genome shotgun sequence.
ACCESSION   DBNHBY010000829 DBNHBY010000000
VERSION     DBNHBY010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_202319"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /gene="trpE"
                     /locus_tag="AC16HB_19295"
     CDS             <1..1484
                     /gene="trpE"
                     /locus_tag="AC16HB_19295"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5608991.1"
                     /translation="AQGYNRIPLIAESFADLDTPLSLYLKLVKSQGASANSYLLESVV
                     GGERFGRYSFIGLPARTALRSCIGEGGIAGTHRTEVVTDGRIVREDRGDPLAFIEKYL
                     AQFKVAVRPGLPRFCGGLAGYFGYDTVRLIEKKLAQPKPDDLGLPDIHLLLTEELAVI
                     DNLSGRAYLIVYADATHPEGYQRARERLRELKARLHRHIEPPHTRASVRRPETREFDK
                     ERYLAAVARSKEYIAAGDVMQVQIGQRISKQYADAPLSLYRALRTLNPSPYMYYYDLG
                     DAHIVGASPEILVRSEVRDDERIVAIRPIAGTRPRGGTPEQDAKLADELAADPKERAE
                     HLMLIDLARNDIGRIAEIGSVKVPEQYVIERYSHVMHLVSHVEGKLKPGLTNLDVLRA
                     TFPAGTLSGAPKVRAMEIIDELEPVKRGIYGGAVGYLSFAGDMDLAIAIRTGVIKGNV
                     LYAQAAAGSVADSVPEMEWRETEAKARAVLRAAETVEDGFDVE"
     gene            1602..>2576
                     /gene="trpS"
                     /locus_tag="AC16HB_19300"
     CDS             1602..>2576
                     /gene="trpS"
                     /locus_tag="AC16HB_19300"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5608992.1"
                     /translation="MSSEQNSVAPRVALLTGLRPTGALTLANVVGAVQPILRLQAATE
                     LPILLFVADLHALTDTEPQAVAQQVQSTVRDLLALGIDTARVHVFVQSALAHEVNELA
                     SFLAREVRVSELLRQPSLKEKLRSGQQPENATAALLLYPVLMAADILLHRAARVPVGQ
                     DQLAHLELARLMARRFNADYGATLVEPQPLEEDAPRIRALRGDGKMSKSDPETALLLS
                     DSDAAIEGKIATCQTAPEGESSPHLDSHALLIESLDPASAPAVRRLLAAHRAGDKVMA
                     DFKRLFASVAKQFVAGVRARRAALTDAAVERALQAGTAFAKQSAAQTLD"
BASE COUNT          446 a          864 c          893 g          373 t
ORIGIN      
        1 ccgcacaggg gtacaaccgc atcccgctga ttgcggagag cttcgccgat ctcgataccc
       61 cgctgtcgct gtacctgaag ctggtcaaga gccaaggcgc gagcgcgaac tcgtacctgc
      121 tcgaatcggt cgtcggcggc gaacgcttcg gccgctattc gttcatcggc ctgccggcgc
      181 gtaccgcgct tcgaagctgc atcggcgagg gtggcatcgc gggcacgcat cgcaccgagg
      241 tcgtcaccga cggccgcatc gtgcgggagg atcgtggcga tccgctcgcg ttcatcgaga
      301 agtacctggc ccagttcaag gtcgcagtgc ggccggggct gccgcgcttc tgcggcgggt
      361 tggccggcta cttcggctac gacacggtac ggctgatcga gaagaaactc gcccaaccca
      421 agcccgacga tctgggcctg cccgacatcc acctgctgct gaccgaggaa ctcgcggtca
      481 tcgacaacct cagcggccgc gcctacctga tcgtgtacgc cgacgcgacg caccccgagg
      541 gttaccagcg cgcgcgcgaa cggctgcggg aactgaaggc gcggctgcac cggcacatcg
      601 agccgccgca cacgcgcgcc agcgtgcgcc ggcccgagac gcgcgagttc gacaaggagc
      661 ggtatctcgc ggcggtcgcg cgcagcaagg aatacatcgc tgccggcgac gtgatgcagg
      721 tacagatcgg ccagcgcatc agcaagcagt acgccgatgc gccactgtcg ctgtatcgcg
      781 cgctgcgcac gctgaacccg tcgccataca tgtactacta cgacctcggc gatgcgcaca
      841 tcgtcggtgc gtcgcccgag atcctggtga ggagcgaagt gcgcgatgac gagcgtatcg
      901 tcgccatccg cccgatcgcc ggcacgcgtc cgcgcggcgg cacgccggag caggacgcga
      961 agctcgccga cgagcttgcc gccgatccca aggagcgcgc cgagcacctg atgctgatcg
     1021 atctggcgcg caacgacatc ggccgcatcg ccgagatcgg cagcgtcaag gtgcccgagc
     1081 agtacgtgat cgagcggtac tcgcacgtga tgcacctggt gagccacgtc gaggggaagc
     1141 tgaagccggg tctgaccaac ctcgacgtgc tgcgggcgac ctttcccgcg ggcacattga
     1201 gcggcgcgcc gaaggtgcgc gcgatggaga tcatcgacga actggagccg gtcaagcgcg
     1261 gcatctacgg cggcgcggtc gggtacctga gcttcgccgg cgacatggat ctggcgatcg
     1321 ccatccgcac cggcgtgata aagggcaacg tgctgtacgc gcaggccgcg gccggcagcg
     1381 tcgccgactc ggtcccggaa atggaatggc gcgagaccga ggccaaggcg cgcgcagtac
     1441 tgcgcgcggc cgagaccgtc gaggacgggt tcgatgtgga atgaacagag gtctggggga
     1501 ggctaacgtg cgaagcacgt tacgcgcgaa gcgcggccga caccaaccgc actcggcgcg
     1561 cgcagtcttg cgcgcggccg agaccgtcga ggacgggttc gatgtcgagt gagcagaatt
     1621 ccgtcgcgcc gcgcgttgcc ctgctgaccg gcctgcgacc gaccggcgcg ctgacgctgg
     1681 ccaatgtcgt cggcgcggtg cagccgatcc tgcgcctgca ggcagcgacc gaactgccga
     1741 tcctgctctt cgttgccgac ctgcacgcgc tgaccgacac cgagccgcag gcggtcgcgc
     1801 agcaggtgca gtcgaccgtg cgcgacctgc tggcgctcgg catcgacacg gcacgggtgc
     1861 acgtgttcgt gcaatcggcg ctcgcgcacg aggtgaatga actggcgagc tttctggcgc
     1921 gcgaggtgcg cgtgtccgag ttgttgcgac aaccgagcct caaggaaaaa ctgcgttcgg
     1981 gccaacagcc cgagaacgcg accgccgcgc tgttgctgta cccggtgctg atggcagccg
     2041 atatcctcct gcatcgcgcg gcgcgggttc ccgtgggaca ggatcagttg gcgcatctcg
     2101 aactggcgcg gctcatggcg cgccggttca atgccgacta cggcgccacg ctggtcgagc
     2161 cgcagccgct cgaggaagac gcgccgcgca tccgcgcact gcgcggcgac ggcaagatga
     2221 gcaagagcga tcccgagacc gcgctgctgc tgtcggattc cgatgcggcg atcgagggca
     2281 agatcgcgac ctgccaaacc gcgccggagg gcgagtcgtc accgcatctg gacagccacg
     2341 cgctgctgat cgagtcgctc gatcccgcca gcgcacccgc cgtgcgccgg ctgctggctg
     2401 cgcaccgcgc gggcgacaag gtcatggcgg acttcaagcg tctgttcgca agcgtcgcca
     2461 agcagttcgt tgccggcgtg cgtgcgcggc gcgcggcact gacggacgct gcggtcgagc
     2521 gcgccttgca ggccggtact gcgttcgcaa agcaaagcgc agcgcagaca ctggac
//
LOCUS       DBNHBY010000830         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1080875, whole genome shotgun sequence.
ACCESSION   DBNHBY010000830 DBNHBY010000000
VERSION     DBNHBY010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1080875"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16HB_19305"
     CDS             complement(<1..185)
                     /locus_tag="AC16HB_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5608993.1"
                     /translation="MLKFLPRRLLQLIPTLFLVSVLIFGLQQLLPGDPALVMAGEERD
                     PAVIEQIRKQYRLDQPLP"
     gene            complement(199..1710)
                     /locus_tag="AC16HB_19310"
     CDS             complement(199..1710)
                     /locus_tag="AC16HB_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5608994.1"
                     /translation="MKTISGILGITLALGVASAVHAQSTLRIGLAEDPDILDPTLART
                     YVGRIVFASICDKLFDIDDKLAVVPQLALSQQTSPDGRTVTIKLRPGVKFHDGEPFNA
                     EAAKFSLERHLNMQGSFRKAEIAQIDKVEVVDPLTIRLQLKAPFAPLIAQLTDRAGMM
                     VSPKAAAALGDKFGTAPVCAGPYKFVERVPQDRIVVERFKDYWDAKRVLIDRIVYRPI
                     QDSTVRLANLKSGQIDLLERLLATDVDEVKKDSKLKLATGTEMGYQGVTFNVNNGDKS
                     NNPLGKNWKVREAFELAIDRDAINQVVFNGLQKPDNQWVSLASPYHMAKFPLPKRDLA
                     KAKALLKEAGVTPPLALDFMVPNNPESRAVAEVMQSMAAEAGFDLKLRVTEFATSLNE
                     AEKGNFQLYFLAWSGRTDPDGNIFSFASCNGPLNYGKYCDPKIDELLTKARTATTHAE
                     RKALYEQAAAEILPKGGVMYLYHRTLLFAHLPRLQGFKVMPDGLIRVTDLQLK"
     gene            complement(1733..>2458)
                     /locus_tag="AC16HB_19315"
     CDS             complement(1733..>2458)
                     /locus_tag="AC16HB_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5608995.1"
                     /translation="LTEPLMLHDVVPSAQRGARVAELLALVGLRADAAQRYPHEFSGG
                     QRQRLAIARALASDPKAIVCDEPVSALDVSIQAQILNLLADLQRRLGLAYVFISHDLA
                     VVRHIATRVAVMYLGRFVETGKTESIFARPRHPYTRALLAAVPIPVPKARVREAVLAG
                     DVPSPLAPPPGCHFHTRCPYAVDRCRTDVPALEPADGTLVACHRWRELDAAPVALLRA
                     GQQHPSLARLQAAFASGRGAQIE"
BASE COUNT          379 a          821 c          835 g          423 t
ORIGIN      
        1 ggcagcggct ggtcgagccg gtactgcttg cggatctgct cgatcaccgc agggtcgcgt
       61 tcctcgcccg ccatcaccag cgccgggtcg cccggcagca actgctgcag gccgaagatc
      121 agcaccgaca ccaggaacag cgtcggaatc agctgcagca ggcggcgggg gaggaacttc
      181 agcaacgcgc gccctgcatc acttcagttg caggtccgtc acccggatca atccgtcggg
      241 catcaccttg aagccctgca gccgcggcaa atgcgcgaac agcagcgtgc ggtgatacag
      301 gtacatcacg ccgcccttcg gcaggatctc cgcggcggcc tgctcgtaca acgccttgcg
      361 ctcggcatgc gtcgtcgcgg tgcgcgcctt ggtcagcagc tcgtcgattt tcggatcgca
      421 gtacttgccg tagttcagcg gcccgttgca cgacgcgaac gagaagatgt tgccgtccgg
      481 atcggtgcgg ccgctccacg cgaggaagta gagctggaag ttgcctttct ccgcttcgtt
      541 cagcgacgtg gcgaactcgg tcacgcgcag cttcagatcg aagccggctt cggccgccat
      601 cgactgcatc acctccgcca ccgcgcggct ctccgggttg ttcggcacca tgaagtccag
      661 cgcgagcggc ggcgtgacgc cggcttcctt cagcagcgcc ttcgctttcg caaggtcgcg
      721 cttcgggagc gggaacttgg ccatgtggta ggggctggcg aggctcaccc actggttgtc
      781 gggtttctgc aggccgttga acacgacctg gttgatcgcg tcgcggtcga tcgcaagttc
      841 gaacgcctcg cgcaccttcc agttcttgcc gagcgggttg ttcgacttgt cgccgttgtt
      901 cacgttgaag gtcacaccct ggtagcccat ctcggtgccg gtcgcgagct tgagcttgga
      961 atccttcttc acctcgtcga cgtcggtggc gagcagccgc tcgagcaggt cgatctggcc
     1021 ggacttcagg ttcgccagcc gcaccgtcga atcctggatt ggccgataca cgatgcggtc
     1081 gatcagcacg cgcttcgcgt cccagtagtc cttgaagcgc tcgaccacga tgcggtcctg
     1141 cggcacgcgc tcgacgaact tgtaggggcc tgcgcatacc ggcgcggtgc cgaacttgtc
     1201 gcccagcgcg gccgccgcct tcggggacac catcatgccg gcgcggtcgg tgagctgcgc
     1261 gatcagcggc gcgaacggcg ccttcaactg cagccggatc gtcagcgggt cgaccacctc
     1321 gaccttgtcg atctgcgcga tctccgcctt gcggaacgaa ccctgcatgt tcaggtgccg
     1381 ctcgaggctg aacttcgccg cttcggcgtt gaacggttcg ccgtcgtgga acttcacgcc
     1441 gggtcgcagc ttgatcgtga ccgtcctgcc gtcgggcgag gtctgctgcg acagcgccag
     1501 ttgcggcacg accgcgagct tgtcgtcgat gtcgaacagc ttgtcgcaga tcgacgcgaa
     1561 cacgatgcgg ccgacgtacg tgcgcgccag cgtcgggtcg aggatgtcgg gatcttccgc
     1621 cagcccgatg cgcagggtgc tttgcgcgtg caccgcactc gcgacgccga gcgcgagcgt
     1681 gattccgaga atgcctgaga tcgtcttcat ctgtgcagcc tttcgaagga aactattcga
     1741 tctgcgcgcc gcggccggaa gcgaatgctg cctgcagccg cgcgagtgac ggatgttgct
     1801 gtcccgcgcg caacagcgca accggcgcgg cgtccagctc gcgccagcga tggcaggcga
     1861 cgagggtgcc gtccgccggt tcgagcgctg gcacgtcggt ccggcagcgg tcgactgcgt
     1921 acgggcagcg cgtatggaaa tggcagccgg gcggcggcgc cagtgggctg ggcacgtcgc
     1981 cggcgagcac ggcttcgcgc acgcgcgcct tcggcaccgg gatcggcacc gcggcgagca
     2041 gcgcacgcgt gtagggatgg cgcggccgcg cgaagatcga ctccgtcttc cccgtctcca
     2101 cgaagcggcc gaggtacatc acggccacgc gtgtcgcgat gtgccgcacc accgcgaggt
     2161 cgtgggagat gaacacgtac gccaggccga ggcgccgctg caggtcggcg agcaggttca
     2221 ggatctgcgc ctggatcgac acgtcgagcg cggacaccgg ctcgtcgcag acgatcgcct
     2281 tggggtcgga agccagcgcg cgcgcgatcg cgagccgctg ccgctgcccg ccggagaact
     2341 cgtgcggata gcgctgtgcg gcgtcggccc tcaggccgac cagtgccagc agttcggcca
     2401 cgcgcgcgcc gcgctgcgcg gacggaacga cgtcgtgcag catcagcggc tcggtcag
//
LOCUS       DBNHBY010000831         4147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1487526, whole genome shotgun sequence.
ACCESSION   DBNHBY010000831 DBNHBY010000000
VERSION     DBNHBY010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1487526"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16HB_19320"
     CDS             <1..440
                     /locus_tag="AC16HB_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR5608996.1"
                     /translation="GGSQGGLLVTTAMVQRPDLFGAVVAQVPLTDMLRYHKLLAGASW
                     VAEYGDPDQQGERAYIEKYSPYQNVKKDVTYPRLFVATSTRDDRVHPGHARKLAARML
                     AQGHDVLYFENIEGGHNAAADNAQRARMWAQTFSFLWRQLAPR"
     gene            442..1221
                     /locus_tag="AC16HB_19325"
     CDS             442..1221
                     /locus_tag="AC16HB_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR5608997.1"
                     /translation="MGVARLRFYEELNDFLAPALRRREFDYAFARAATVKNAIEALGV
                     PHTEVELILVNGRSVDFAHRMKDGDRVSVYPVFETFDIAPLVRVRERPLRRMRFIADS
                     HLGGLARLLRMAGFDTLHDNHYADDEIRGIAAREGRTVLTRDRELLKCRDVTHGCYVH
                     ALRSAQQLREIVERLQLAGSMAPFTLCLHCNLPLAEVSKADVLARLPPMVAANYNRFR
                     TCSGCRRVYWEGSHWRRMRELIEKIVPSSEPPDPDVSIAPR"
     gene            complement(1440..2609)
                     /gene="dnaJ"
                     /locus_tag="AC16HB_19330"
     CDS             complement(1440..2609)
                     /gene="dnaJ"
                     /locus_tag="AC16HB_19330"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5608998.1"
                     /translation="MAKRDFYEILGVAKNASEEEIKKAYRKLAMKYHPDRNQGKGEEK
                     STKAAEEKFKEVKQAYEVLTDANKRAAYDRFGHAGVDPSAAGGGFGGGGPGFGNFADA
                     FGDIFSDIFGGAMGGAAGRGGRSAAYRGADLRYNMEITLEQAAKGFATDIRVPSWDAC
                     ETCHGTGAKPGTSAKTCGTCSGSGAVRMTQGFFSIQQTCPTCHGSGKVIAEPCVTCHG
                     EGRVKKNKVLEVNIPAGIDEGQRIRLSGKGEPGLNGGPPGDLYVEIRIKPHDVFQRDG
                     DDLHCEVPVSVVTAALGGEVDVGTLDGKVSLSVPEGTQSGKTFRLRGKGVKGVRSSYP
                     GDLYCHINVETPVRLTEKQKKMLRDFDASLREGGSKHSPQTKGFMDKMKGFFTAE"
     gene            complement(2700..>4147)
                     /gene="dnaK"
                     /locus_tag="AC16HB_19335"
     CDS             complement(2700..>4147)
                     /gene="dnaK"
                     /locus_tag="AC16HB_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR5608999.1"
                     /translation="RIINEPTAAALAFGLDKHGKGDRKIAVFDLGGGTFDISIIEIAD
                     VEGEKQFEVLSTNGDTFLGGEDFDQRIIDYVVTEFKKESGADLSKDVLALQRLKDAAE
                     KAKIELSSGQQTEINLPYITADASGPKHLNMKLTRSKLEALVEDLIERTIEPCRIAIK
                     DAGVKVGDIQDVILVGGQTRMPKVQDKVREFFGKEPRKDVNPDEAVAVGAAIQGSVLS
                     GDRTDVLLLDVTPLSLGIETLGGVMTKMIQKNTTIPTKYSQVFSTADDNQPAVTIKVF
                     QGEREMATGNKLLGEFNLEGIPPAPRGMPQIEVTFDIDANGILHVSAKDKASGKENKI
                     TIKANSGLTEDEIKRMVADAESHKAEDHKRFELAQARNTADASVHQIKKALAEHGSAL
                     DAGEKGKIEDAIKAVEEALKGDDKDAMEARTAAMMTAAQKLGEKMYASTQGATGAADA
                     GSSAGAQAAGEAKAAKNEDVVDADYKEVKRG"
BASE COUNT          588 a         1423 c         1321 g          815 t
ORIGIN      
        1 tcggcggcag ccagggcggc ctgctggtca cgacggccat ggtgcagcga ccggatctct
       61 tcggcgccgt ggtcgcgcag gtgccgctca cggacatgct gcgctatcac aagctgctcg
      121 ccggcgcctc ctgggtcgcc gagtacggcg acccggacca gcagggcgag cgcgcataca
      181 tcgagaaata ctccccctac cagaacgtga agaaggatgt gacgtatccg cgcctttttg
      241 tggcgacctc gacgcgcgac gaccgcgtgc atccgggcca cgcgcgcaag ctggcggcga
      301 ggatgctcgc gcagggccac gacgtgctgt acttcgagaa catcgagggc ggacacaacg
      361 ccgccgccga taacgcgcag cgggcacgga tgtgggcgca gacgttctcc ttcctgtggc
      421 gccagctcgc gccgcgttga gatgggcgtg gcgcgcctgc gcttctacga ggaactgaac
      481 gacttcctcg cgcccgcgct gcggcggcgc gagttcgact acgcgttcgc ccgggcggca
      541 acggtgaaga acgcgatcga ggcgctcggc gtgccgcaca ccgaagtcga actgatcctc
      601 gtcaatggcc gctcggtcga ttttgcacac cggatgaagg acggcgatcg cgtcagcgtc
      661 tacccggtgt tcgagacatt cgacatcgcg ccgctggtgc gggtgcgcga gcgaccgttg
      721 cggcgtatgc gcttcatcgc cgattcgcat ctcggcggac tcgcgcgcct gctgcggatg
      781 gccggcttcg atacgctgca cgacaaccac tacgcggatg acgagattcg cggcatcgcc
      841 gcgcgcgagg ggcgcaccgt gctcacgcgc gaccgcgaac tgctgaagtg ccgcgacgtg
      901 acgcacggct gctacgtgca tgcgctgcgg tcggcgcagc agttgcgcga gatcgtcgag
      961 cggctgcagc tcgccggcag catggcgccg ttcacgctgt gcctgcactg caacctgccg
     1021 ctggcggagg tcagcaaggc ggacgtgctg gcgcggctgc cgccgatggt ggcggcgaac
     1081 tacaaccgtt tccgcacctg ctcgggctgc cggcgtgtct actgggaagg cagtcactgg
     1141 cggcggatgc gcgagctgat cgagaagatc gtgccctcgt ccgaaccgcc cgatccggac
     1201 gtgtccatcg ctccgcggtg agaaagcgcg ctccgcgcgc ggatcttgtg gctgcggccg
     1261 tcgctgcgcg acttgacttc ggctgcgccg aagtcagcct gcgccgcaag atgaaagggt
     1321 tcttcactct gcggtgaagc agcgcgctcc gcgcgcaaat cttgtggctg cggccgtcgc
     1381 tacgcgactt gacttcggct gcgccgaagt cagcctgcgc cgcaagatga aagggttctt
     1441 cactctgcgg tgaagaaccc tttcatcttg tccatgaagc ctttggtttg cggcgagtgt
     1501 tttgagccgc cctctcgcag agaagcgtcg aaatcgcgca gcatcttctt ctgcttctcc
     1561 gtcagtcgca ccggggtttc gacgttgatg tggcagtaca ggtcgccggg gtagctcgag
     1621 cgcacgccct tgacgccctt gccccgcagg cggaaggtct tgccgctctg cgtgccctcg
     1681 ggtaccgaca ggctcacctt gccgtcgagc gtgccgacgt cgacctcgcc gccgagcgcc
     1741 gcggtgacga ccgacaccgg cacttcgcag tgcaggtcat cgccatcgcg ctggaacacg
     1801 tcgtgtggct tgatgcggat ctcgacgtac agatcccccg gcggaccgcc attgaggccg
     1861 ggctcaccct tgcccgacag ccggatgcgc tggccctcgt cgatgccggc cgggatgttc
     1921 acctcgagca ccttgttctt cttcacccgg ccttcgccgt ggcaggtcac gcacggctcg
     1981 gcaatcacct tgccgctgcc gtggcaggtc gggcaggtct gctggatcga gaagaagccc
     2041 tgcgtcatgc gcactgcgcc cgagccgctg caggtgccgc aggtcttggc cgacgtgccg
     2101 ggcttggcac ccgtgccgtg gcaggtctcg cacgcgtccc agctcggcac gcgaatgtcg
     2161 gtggcgaatc ccttcgcggc ctgctcgagc gtgatttcca tgttgtagcg gaggtccgcg
     2221 ccgcgatacg cggccgaccg gccaccgcga cccgccgcgc cgcccatcgc gccgccgaag
     2281 atgtcgctga agatgtcgcc gaacgcatcg gcgaaattgc cgaaccccgg tccgccgccg
     2341 ccaaagccgc cgcccgccgc gctcgggtcc acgcccgcat ggccgaagcg gtcgtatgcg
     2401 gcgcgcttgt tcgcgtcggt cagtacttca tacgcctgct tgacctcctt gaacttctct
     2461 tcggccgcct tggtggactt ctcctcaccc ttgccctgat tgcggtcagg gtggtacttc
     2521 atcgccagct tgcgataagc cttcttgatt tcctcttcgc tggcgttttt cgccacgccg
     2581 aggatttcgt agaaatcacg tttggccatt gttcttccag agcccttcaa atggcaacgc
     2641 cgagccgagc ggcgctggtt cgtccgctcg aactcggcga gtcaaacccg gcagcgaagc
     2701 tatccgcgct tgacctcttt gtagtcggcg tcgaccacgt cttcgttctt cgccgccttg
     2761 gcctctcccg ccgcctgcgc accggcggag gaacccgcat ccgcggcccc cgtcgcgccc
     2821 tgcgtgctcg catacatctt ctcgcccagc ttctgggcgg cggtcatcat cgccgcggtc
     2881 ctggcctcca tcgcgtcctt gtcgtcgccc ttgagtgcct cttcgacggc cttgatcgca
     2941 tcttcgatct tccccttctc gccggcgtcg agcgccgaac cgtgctcggc gagcgccttc
     3001 ttgatctggt gcacgctcgc atcggcggta ttgcgcgcct gcgccagttc gaagcgcttg
     3061 tggtcctcgg ccttgtgcga ctcggcgtcg gcgaccatgc gcttgatctc gtcctcggtc
     3121 aggccggagt tggccttgat cgtgatcttg ttctccttgc cggaggcctt gtccttggcg
     3181 ctgacgtgca ggatgccgtt ggcgtcgatg tcgaacgtga cctcgatctg cggcatgccg
     3241 cgcggcgcgg gcgggatgcc ctcgaggttg aactccccga gcagcttgtt gccggtggcc
     3301 atctcgcgct cgccctggaa caccttgatc gtcaccgccg gctggttgtc gtcggccgtc
     3361 gagaacacct gcgagtactt ggtcggaatc gtggtgttct tctggatcat cttggtcatc
     3421 acgccgccca gcgtctcgat gccgagcgac agcggcgtga catcgagcag cagaacgtcg
     3481 gtgcggtcgc cggacagcac gctgccctgg atcgcggcgc cgaccgccac cgcctcgtcc
     3541 gggttgacgt ccttgcgtgg ctccttgccg aagaactcgc gcaccttgtc ctgcaccttc
     3601 ggcatgcgcg tctggccgcc gaccaggatc acgtcctgga tgtcgccgac cttcacgccg
     3661 gcgtccttga tcgcgatccg gcacggttcg atcgtgcgct cgatcagatc ctcgaccagc
     3721 gcctcgagct tggagcgcgt gagcttcatg ttcaggtgct tcgggcccga ggcgtcggcc
     3781 gtgatgtacg gcaggttgat ctcggtctgc tggcccgacg acaactcgat cttcgccttc
     3841 tcggcggcgt ccttcagccg ctgcagcgcg agcacgtcct tcgaaagatc cgcgccgctc
     3901 tccttcttga actcggtgac cacgtaatcg atgatgcgct ggtcgaaatc ctcgccgccg
     3961 aggaacgtgt cgccattcgt cgacagcacc tcgaactgct tctcgccttc cacgtccgcg
     4021 atctcgatga tcgagatgtc gaacgtgccg ccgccgaggt cgaacaccgc gatcttgcgg
     4081 tcgcccttgc catgcttgtc caggccgaat gcgagcgcgg ccgcggtcgg ctcgttgatg
     4141 atgcgct
//
LOCUS       DBNHBY010000832         4217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_743499, whole genome shotgun sequence.
ACCESSION   DBNHBY010000832 DBNHBY010000000
VERSION     DBNHBY010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_743499"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16HB_19340"
     CDS             complement(<1..191)
                     /locus_tag="AC16HB_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5609000.1"
                     /translation="MNARLDFKAATAHVDEAAVLPLPASRKVYVTGSRPDIRVPMREI
                     TQNDTPIFGAAAQGGAPARL"
     regulatory      complement(232..329)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(388..1131)
                     /locus_tag="AC16HB_19345"
     CDS             complement(388..1131)
                     /locus_tag="AC16HB_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5609001.1"
                     /translation="MSDVILHVRDLCVAYGKVEALHNANLKVGMGQIVTVIGPNGAGK
                     TTMLAAIMGALPLNGSTRGEILFGGRQCAGTEIEQMVAAGMTLVPEKRELFGEMTVED
                     NLLLGGFQRYRGGSRDQAPTMQEVFALFPRLQERRAQLAGTLSGGERQMLAIGRALMA
                     KPKLLMLDEPSLGLAPLIVREIFHIIEELKRRGSSILLIEQNARAALKVADYAYVLET
                     GEIALEGPAAELASDTRVIESYLGLGGKR"
     gene            complement(1198..2985)
                     /locus_tag="AC16HB_19350"
     CDS             complement(1198..2985)
                     /locus_tag="AC16HB_19350"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR5609002.1"
                     /translation="MLLAFLVALGLAPLVLPEFTITLANYIGLYALVALGLVLLTGVG
                     GLTSFGQAAFVGLGAYTTAVLTTATDLPAWLAWIGASTWLALLAGLLLTLAFAVAIGM
                     LTLRLSGHYLPLGTIGWGISLFYVFGNLQLLGGFTGITGVPPISIFGHAIISGREIFY
                     LIWLFLLAGVVTTQNLLDSREGRAIRAVRGARVMAESMGVNTADSRLAIFVLAALQAC
                     ASGWLYAHMQRFLNPTPFSINVGIEYLFMAVVGGAGEVWGALLGATVITVAKQWLQDI
                     LPQIVGQAGNFEVIVFGIVMVIVLQRARDGLWPVLTKWVPLARRPRTIDPGVPPLARR
                     DKPAAGETLLEVSGATMRFGGLVAVNDMALKVKAGEIVALIGPNGAGKSTTFNVISGV
                     LAATEGDIRFRGQALRGMSARAIAALGMSRTFQHVKLLPLMSVLDNVAIGAHARENPG
                     LFRGVVAGAWRLERRDEMRLLAESARQIERVGLADHMFEAAGSLPLGKQRLLEVARAL
                     AADPCLLLLDEPAAGLRYLEKRALAELIRKLKAEGIAILLVEHDMDFVMGLVDRVYVM
                     DFGQKICEGVPEQVQRDPRVLEAYLGGVE"
     gene            complement(3101..4138)
                     /locus_tag="AC16HB_19355"
     CDS             complement(3101..4138)
                     /locus_tag="AC16HB_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5609003.1"
                     /translation="MDFQIALLLGQDGVTNGAIYALLALALVLAFAVTRVIFIPQGEF
                     VAYGALTLVALQAGKLPGTIWLLLIAGTLVALIDLVGAVRARRWSGLPALLGWNVAYP
                     LALLALIRWVPLQGAPMPVQILLTLAVVVPLGPMLYRLAYQPLAEASVLVLLILSVAV
                     HVVMVGLGLLLFGAEGSRTPAFSEGRFEFGGMTVTAQTLWVIGCSIALIVILYLLFER
                     TLYGKALRATAINRTGARLMGIPTALAGKLSFFLCALIGAFSGILIAPITTIYYDTGF
                     LIGLKGFVGAIIGGLASYPIAALGAVLVGLLESYSSFWASAYKEAIVFTLIIPVLLWR
                     SLTTHHVEEEE"
BASE COUNT          737 a         1506 c         1380 g          594 t
ORIGIN      
        1 agacgcgcag gggctcctcc ctgcgcggcg gcgccgaaga tcggcgtgtc gttctgcgtg
       61 atctcgcgca tcggcacgcg gatgtccggg cgcgagccgg tcacgtacac cttgcgcgac
      121 gcgggcagcg gcagcaccgc ggcttcgtcg acgtgcgcgg ttgcggcttt gaaatcgagg
      181 cgggcgttca tcgggctctc cttggtgcgg agagccaacg ggcggaaacg gggagtcttc
      241 cgcgcgtttc cctccgccgg tattagccgg ttcaggttcg aggggactgt ctcaactgcg
      301 ggccttccgc agtaccccca acgcatcgag gcaacgatag tccgccgtgg gcagcgacgc
      361 aaatccggcg ccgcgccccg caacggatca gcgcttgccg ccgagtccga gatagctttc
      421 gatcacgcgc gtgtcgctgg cgagttcggc ggccggacct tcgagcgcga tctcgccggt
      481 ttccagaacg tacgcgtagt cggcgacctt gagcgctgcg cgcgcgttct gctcaatcag
      541 caggatcgac gagccgcgcc gcttcagctc ctcgatgatg tggaagatct cgcgcacgat
      601 cagcggcgcc agccccagcg aaggctcgtc gagcatcagc agcttcggct tggccatcag
      661 cgcgcgaccg atcgcgagca tctgccgttc gccgccggac agcgtgccgg cgagctgcgc
      721 gcgccgctcc tgcaggcgcg gaaagagcgc gaacacctcc tgcatcgtcg gcgcctggtc
      781 gcggctgccg ccgcgatagc gctggaagcc gccgagcagc aggttgtcct cgaccgtcat
      841 ctcaccgaac agctcgcgct tttcgggcac cagcgtcatg ccggcggcga ccatctgctc
      901 gatctccgtg ccggcgcact gccggccgcc aaacaggatc tcgccgcgcg tcgagccgtt
      961 cagcggcagc gcgcccatga tcgccgcgag catcgtcgtc ttgcccgcgc cgttgggccc
     1021 gatcacggtg acgatctggc ccatgccgac cttcaggttc gcgttgtgca gcgcctcgac
     1081 cttgccgtag gccacgcata ggtcacgcac gtgcaggatc acgtcgctca ctcgacgcct
     1141 cccgacggcc gggcacgcgg cgtcattcga cgcctcccaa tggccgggca cgcggcgtca
     1201 ttcgacgcct cccagatatg cctcgagcac gcgcggatcg cgctgcacct gctcgggtac
     1261 gccttcgcag atcttctggc cgaagtccat cacgtagacg cgatcgacca gccccatcac
     1321 gaagtccatg tcgtgctcga cgagcaggat cgcgatgcct tcggccttca gtttccgtat
     1381 cagctcggcg agtgcgcgct tctcgaggta gcgcagaccg gccgccggct cgtcgagcag
     1441 cagcaggcac ggatcggccg cgagcgcgcg tgcgacctcg agcagccgct gcttgccgag
     1501 cggcagcgag ccggcggctt cgaacatgtg atcggccagg ccgacgcgct cgatctggcg
     1561 cgccgattcg gccagcagcc gcatctcgtc gcgccgctcg agccgccacg cgccggcgac
     1621 cacgccacgg aacagccccg ggttctcgcg cgcatgcgcg ccgatcgcga cgttgtcgag
     1681 cacgctcatc agcggcagca gcttcacgtg ctggaaggtg cggctcatgc cgagcgcggc
     1741 gatcgcgcgc gccgacatgc cgcgcagcgc ctggccgcgg aagcggatgt cgccttcggt
     1801 cgcggcgagc acgccggaga tgacgttgaa ggtcgtgctc ttgccggcgc cgttgggacc
     1861 gatcagcgcg acgatctcgc ccgccttcac cttcagcgcc atgtcgttga cggcgaccag
     1921 cccgccgaag cgcatcgtcg cgccgctgac ctcgagcagt gtctcgcccg cggccggctt
     1981 gtcacggcgc gccagcggcg gcacgcccgg atcgatggtg cgcggccgcc gcgcgagcgg
     2041 cacccacttc gtcagcaccg gccacagtcc atcacgcgcg cgctgcagca cgatcaccat
     2101 cacgatgccg aacacgatca cttcgaagtt gccggcttgg ccgacgatct gcggcaggat
     2161 gtcctgcagc cactgcttgg ccacggtgat gacggtcgcg ccgagcagcg cgccccacac
     2221 ttcgccggcc cctccgacca ccgccatgaa cagatactcg atgccgacat tgatcgagaa
     2281 cggcgtcggg ttcaggaagc gctgcatgtg cgcgtacagc caacccgagg cgcaggcctg
     2341 cagcgcggcg agcacgaaga ttgcaagccg cgagtccgcg gtgttgacgc ccatcgactc
     2401 ggccatcacg cgcgcgcccc tgaccgcgcg gatcgcacgg ccttcacgcg agtcgagcag
     2461 attctgcgtg gtgaccacgc cggccagcag aaacagccag atcaggtaga agatctcgcg
     2521 cccgctgata atcgcgtgcc cgaagatcga aatcggcggc acgccggtga tgccggtgaa
     2581 gccgccgagc agttgcaggt tgccgaacac gtagaacagg ctgatgcccc agccgatcgt
     2641 gcccagcggc aggtagtgac ccgacagtcg cagcgtcagc atgccgattg ccaccgcaaa
     2701 cgcgagcgtc agcagcagcc ctgcgagcag cgcgagccag gtcgacgcgc cgatccacgc
     2761 cagccaggcc ggcagatcgg tcgcggtcgt cagcaccgcc gtcgtgtacg caccgaggcc
     2821 gacgaacgcg gcctgtccga acgaggtcag cccgcccacg ccggtcagca gcaccagccc
     2881 gagcgccacc agcgcgtaca acccgatgta gttggcgagc gtgatcgtga actcgggcag
     2941 caccagtggc gccagcccca gcgccacgag gaacgccagc agcatgcggc cgggcgtcac
     3001 gacctgacct cgatgcgata cgcgagccga aacaagtgcg caagtgctcc gcccgcggcg
     3061 caggcgtgga atccaagggg gcagcagccc tcttgcatcc ctactcctcc tcctcgacgt
     3121 ggtgcgtggt cagcgaccgc cacagcagca cggggatgat tagcgtgaag acgattgctt
     3181 ccttgtatgc gctggcccag aacgacgaat acgactccag caggcccacg agcaccgcgc
     3241 cgagcgcggc gatcgggtag ctggcgagcc caccgatgat ggcgccgacg aatcccttca
     3301 ggccgatcag gaagccggtg tcgtagtaga tcgtcgtgat cggtgcgatc aggatgccgg
     3361 agaacgcgcc gatcagcgcg cacaggaaga acgagagctt gcccgcgagc gcggtcggga
     3421 tgcccatcag ccgcgcgccc gtgcgattga tcgcggtggc gcgcagcgcc ttgccgtaca
     3481 gcgtgcgctc gaacaacagg tacaggatca cgatgagcgc gatcgagcag ccgatcaccc
     3541 acagcgtctg cgcggtgacc gtcatgccgc cgaactcgaa ccgcccttcg gagaaggcgg
     3601 gcgtgcgcga accttcggcg ccgaagagca gcaggcccag gcccaccatc accacgtgca
     3661 ccgccaccga caggatcagc agcaccagga ccgatgcctc cgccagcggc tggtaggcga
     3721 gccgatagag catcggtccc agcggcacca cgaccgccag cgtgagcagg atctgcaccg
     3781 gcatcggcgc gccctgcagc ggcacccagc ggatcagcgc cagcagcgcg agcggatacg
     3841 ccacgttcca gccgagcaat gccggcaggc cgctccagcg ccgcgcacgc acggcgccga
     3901 cgagatcgat cagcgccacc agcgtgccgg cgatcagcag cagccagatc gtgccgggca
     3961 acttgccggc ctgcagcgcg accagcgtca gcgcgccgta ggcgacgaat tcgccctgcg
     4021 gtatgaagat gacgcgcgtg accgcgaacg ccagcaccag cgcaagcgcc agcagcgcat
     4081 agatcgcgcc attggtgacg ccgtcctgcc cgagcagcag ggcgatctga aaatccatcg
     4141 tgaaccgtgg ttcgttgtgc ggtcaccgcg atccgcggcg caccgcccac ggatcacgga
     4201 tcaccggtca cggatca
//
LOCUS       DBNHBY010000833         1876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_134705, whole genome shotgun sequence.
ACCESSION   DBNHBY010000833 DBNHBY010000000
VERSION     DBNHBY010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_134705"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..553
                     /locus_tag="AC16HB_19360"
     CDS             89..553
                     /locus_tag="AC16HB_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4863 family protein"
                     /protein_id="HIR5609004.1"
                     /translation="MTPEQFTALIAQVTSTLVGKPLDAALEAELNARCPAGSATFEAI
                     RAACRDAVAAGWMCNREAGGIKFGRVIKPGEATHGFSVDVVEMNDCVGPHHRHPNGEI
                     DMVMPLEGDARFDGTPAGWKVYGPGSAHHPTVTGGRAHVLYLLPQGAIEFTK"
     gene            564..>1876
                     /locus_tag="AC16HB_19365"
     CDS             564..>1876
                     /locus_tag="AC16HB_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR5609005.1"
                     /translation="MPVVSRADHSVSPPRIDIPRDYNAAEDLIERNLVAGRAEKVAYI
                     DDRGRTTYGELARRVNRFANLLAALDVHPEERVFLCLLDTIDFPTCFLGAIKAGAVPV
                     AANTLLTTRDYDYMLRDSRARLLVVSAALWPTLAPIVGNITTLKRIVISDGEVEGHSS
                     LDQLMERASTEHQTAPTTCDDPCFWLYSSGSTGAPKGTVHIQSSLTNTAELYGRPILG
                     IREDDIVFSAAKLFFAYGLGNALTFPLSVGATTVLMAERPTPASVFKRIVDHRITIFY
                     GVPTLYAALLASPDAPTREQVAMRRCASAGEALPEEIGKRWSQRFGVDILDGIGSTEM
                     LHIFLSNRPGDVRYGTTGKPVPGYEVRIVDDDGKPVPRGELGELQIKGPTAAAMYWNN
                     RARTRSTFVGEWTRSGDKYREIEGGYFVYSGRSDDMLKVSGIYVSP"
BASE COUNT          309 a          661 c          632 g          274 t
ORIGIN      
        1 cgccgagtcg cgctgcaggg atcggccgcg cggcacgacg cgctcggacg cgacgccgcc
       61 gacgcggcag cgctgtggag ttgagcgaat gacgcccgaa cagttcacgg cattgatcgc
      121 gcaggtcacg tcgaccctcg ttggcaagcc gctcgacgcc gcgctcgagg cggaactgaa
      181 cgcgcgctgc ccggcgggca gtgcgacctt cgaggcgatc cgcgccgcgt gcagggacgc
      241 ggttgccgca ggctggatgt gcaatcgcga ggcgggcggc atcaagttcg gccgcgtcat
      301 caagccgggc gaggccacgc atggcttctc ggtcgacgtg gtcgagatga acgactgcgt
      361 cggcccgcac caccgccacc cgaacggtga aatcgacatg gtgatgccgc tcgaaggcga
      421 cgcgcgcttc gacggcaccc cggccggctg gaaggtctac ggcccgggca gcgcacacca
      481 tccgaccgtc accggcggac gcgcgcacgt gctctatctg ctgcctcagg gcgccattga
      541 attcacgaaa tgagggggat caaatgccgg tcgtcagccg tgccgatcat tcggtgagtc
      601 cgccccgcat cgacattccg cgcgactaca acgccgccga agacctgatc gagcgcaacc
      661 tcgtcgccgg gcgggccgag aaggtcgcgt acatcgacga ccgcggccgc accacgtacg
      721 gcgaactggc gcggcgcgtg aaccgcttcg ccaacctgct cgccgccctc gacgtgcacc
      781 ccgaggagcg cgtgttcctg tgcctgctcg acacgatcga tttcccgacc tgcttcctcg
      841 gcgcgatcaa ggcgggtgcg gtgccggtcg cggccaacac gctgctgacc acgcgcgact
      901 acgactacat gctgcgcgac agccgcgcac ggttgctggt ggtgtccgcc gcgctgtggc
      961 cgacgctggc gccgatcgtc ggcaacatca cgacgctgaa gcggatcgtg atctccgacg
     1021 gcgaggtcga gggccattcg tcgctggacc aactgatgga gcgcgcctcg accgagcacc
     1081 agacggcgcc gaccacctgc gacgacccct gcttctggct gtattcgtcg ggttccaccg
     1141 gcgcgccgaa gggcacggtg cacatccagt cgagcctgac caacaccgcg gaactgtacg
     1201 gccggccgat cctcggcatt cgcgaggacg acatcgtgtt ttcggccgcc aagctcttct
     1261 tcgcctacgg gctcggcaat gcgctcacgt ttccgctgtc ggtcggcgcg accaccgtgc
     1321 tgatggccga gcggcccacg ccggcatccg tgttcaagcg catcgtcgac caccggatca
     1381 cgatcttcta cggcgtgccg acgctgtacg cggcgctgct cgcctcgccc gacgcgccga
     1441 cgcgcgagca ggtggcgatg cgccgctgcg cgtcggccgg cgaggcgctg ccggaggaga
     1501 tcggcaagcg ctggtcgcag cggttcggcg tcgacatcct cgacggcatc ggctcgaccg
     1561 agatgctgca catcttcctg tccaaccgcc ccggcgatgt gcgctacggc acgaccggca
     1621 agccggtccc cggctacgaa gtgcgcatcg tcgacgacga cggcaagccc gtgccgcgcg
     1681 gcgaactggg cgaactgcag atcaagggcc cgaccgcggc ggcgatgtac tggaacaacc
     1741 gcgccaggac gcgctcgacc ttcgtcggcg agtggacgcg cagcggcgac aagtaccgcg
     1801 agatcgaggg cggctatttc gtctacagcg gccgcagcga cgacatgctg aaggtcagcg
     1861 gcatctacgt gtcgcc
//
LOCUS       DBNHBY010000834         2689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1014673, whole genome shotgun sequence.
ACCESSION   DBNHBY010000834 DBNHBY010000000
VERSION     DBNHBY010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1014673"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16HB_19370"
     CDS             complement(<1..523)
                     /locus_tag="AC16HB_19370"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR5609006.1"
                     /translation="MSSALIRNSPCVAPPGRRLDHRMRRRQKKFARGVVGDDPVIYVR
                     NARDSRQRGSNQRSQTLSIEARGVTHGPAGDGSAVKEIAMAAMTKSVLPAAVLALGAL
                     ASGAAFAADVKVTLSGANEVPAVTTSASGDGTISIADDGTVTGSVTTKGIQGTMAHIH
                     LGAADKNGPVVVPF"
     gene            507..1298
                     /locus_tag="AC16HB_19375"
     CDS             507..1298
                     /locus_tag="AC16HB_19375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR5609007.1"
                     /translation="MSAEDIFGLLVPATFIAMWAIEAKWPARRFPHPRGRRLIGIGFF
                     LLMAVVTTIVPLAIPAEWLERHRLLDFSGLGVIAGAVIGYVVLSFVSYAWHRSAHRFA
                     PMWRLFHQVHHSPQYVDMSGAMIFHPTEMVVFALLTIVTTTLVLGLDPVAAALTGYIA
                     AFYSLFQHWNIRTPRWLGYLIQRPEAHCVHHQREVHHYNFADLPLWDILAGTFRNPPS
                     WQGEVGFEPEASRRLGAMLAFRDVNAVDYGPASLGSKDKARVAII"
     gene            complement(1299..1922)
                     /locus_tag="AC16HB_19380"
     CDS             complement(1299..1922)
                     /locus_tag="AC16HB_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR5609008.1"
                     /translation="MHIAILTFDGFNELDSLVALGILNRVRQPGWRATLACPAPTVTS
                     MNGVVLHAQSSLDDACAADAVIVGSGVRTREIANDAAMLSALRLDPARQLIGAQCSGT
                     LLLAKLGLLRGVPACTDLTTKPWVQEAGVEVIDRPFHANGKVATAGGCLASQYLAAWI
                     IARSEGTDAAASALHYVAPVGEKDEYVRRALQNIAPFLAQPQATAAE"
     gene            complement(1938..>2689)
                     /locus_tag="AC16HB_19385"
     CDS             complement(1938..>2689)
                     /locus_tag="AC16HB_19385"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5609009.1"
                     /translation="QLRARFDRDRLMRDGSVLQAVAMVAVGFAPSAWLAAPAMFVAGA
                     AWLAVVNTLTVSAQLVLPNWVRARGMSIYQVAIMGASAASAALWGQVATLADVSVSFV
                     AAAATGLVALFATRHLHEGLGEPVDLTPGRYLDEPVPAFPIEHEQGPVMVTIEYRIDP
                     VRAEEFAVVMRETRADRLRKGALSWGLFEDTTARGRYLEYFLDESWAEHLRRFERMTA
                     SDWELRARRNAFHVGPGDPRITRYLAHSIQR"
BASE COUNT          391 a          981 c          901 g          416 t
ORIGIN      
        1 tgaacggaac gacgacgggg ccgttcttgt cggccgcgcc gaggtgaatg tgcgccatcg
       61 tgccctgaat gcccttggtc gtgacgctgc cggtgaccgt accgtcgtcg gcgatggaga
      121 tcgtgccatc cccgctggcc gacgtcgtga ccgctggcac ctcgttggcg ccggacagcg
      181 tgaccttcac gtcagcggcg aacgcggcgc cgctcgccag cgcgcccagc gcgagaactg
      241 ctgcgggcag caccgatttc gtcattgcag ccattgcaat ctccttgacg gccgaaccgt
      301 ctccggccgg cccatgggtt acacctcgcg cttcgattga caacgtctgg gaacgctggt
      361 tgctcccgcg ctggcgggag tcgcgcgcgt tccgtacgta gatcaccgga tcgtcaccaa
      421 cgacgccgcg cgcgaacttt ttctgccgcc gccgcatcct gtgatcgagc cgccgcccgg
      481 gcggcgccac acagggagaa ttgcgaatga gcgcggaaga catcttcggt ctgctggtgc
      541 cggccacctt catcgcgatg tgggccatcg aagcgaagtg gccggcgcgt cgctttcctc
      601 accccagggg ccggcgcctg atcggcatcg gcttcttcct cctgatggcg gtggtcacca
      661 cgatcgtgcc gctcgcaatc ccggcggagt ggctggagcg ccatcggctg ctcgacttca
      721 gcgggctcgg cgtgatcgcc ggcgcggtaa tcggctacgt cgtgctgtcg ttcgtgtcgt
      781 acgcgtggca ccgcagcgcg caccgctttg cgccgatgtg gcggctgttc caccaggtcc
      841 accacagccc gcagtacgtg gacatgtcgg gcgcgatgat cttccatccg accgagatgg
      901 tcgtattcgc gctgctgacg atcgtgacga cgacgctggt gctcgggctc gatccggtcg
      961 ccgccgcgct gaccggctac atcgctgcct tctacagcct gttccagcac tggaacatcc
     1021 gcacgccgcg ctggctcggc tacctgatcc agcgccccga ggcgcactgc gtgcaccacc
     1081 agcgcgaggt gcaccactac aacttcgccg accttccgct ctgggacatc ctcgccggca
     1141 cgttccgcaa tccgccgtca tggcagggcg aggtcgggtt cgagccggag gcgtcgcgcc
     1201 gtctcggcgc gatgctggcg ttccgcgacg tcaacgcggt cgactacggg ccggccagcc
     1261 tcggcagcaa ggacaaggcg cgcgtcgcca tcatctgatc attccgccgc cgtggcctgc
     1321 ggctgcgcga ggaacggcgc gatgttctgc agcgcgcggc ggacgtactc gtccttctcg
     1381 cccaccggcg cgacgtagtg cagcgcgctc gccgcggcgt ccgtgccctc gctgcgcgcg
     1441 atgatccacg ccgcgaggta ctgcgacgcg aggcagccgc cggcggtggc cactttgccg
     1501 ttcgcgtgaa acggccggtc gatcacctcg acgccggctt cctgcaccca gggcttcgtc
     1561 gtcaggtcgg tgcaggccgg cacgccgcgc agcaggccga gcttcgccag cagcagcgtg
     1621 cccgagcact gcgcgccgat cagctgccgc gccgggtcca gcctgagcgc agacagcatc
     1681 gccgcgtcgt tcgcgatctc gcgcgtccgc acgccgctgc cgacgatcac ggcgtccgcg
     1741 gcgcacgcgt cgtcgagcga cgactgcgcg tgcagcacga cgccgttcat cgacgtcacg
     1801 gtcggcgcgg ggcacgcgag cgtggcgcgc cagcccggct gccggacgcg gttcaggatg
     1861 ccgagcgcaa ccagcgagtc gagttcgttg aaaccgtcga aggtcaggat cgcgatgtgc
     1921 atggcgggac tcgctggcta acgctggatc gaatgcgcaa gatagcgcgt gatcctcggg
     1981 tcgccggggc cgacgtgaaa cgcgttgcgc cgcgcgcgca gctcccagtc ggacgcggtc
     2041 atgcgctcga agcgacgcag gtgctcggcc cacgactcgt cgagaaagta ttcgaggtag
     2101 cggccgcgtg ccgtcgtgtc ctcgaacagc ccccacgaca gcgcaccctt gcgcagccgg
     2161 tcggcgcggg tctcgcgcat cacgacggcg aactcctcgg cgcggaccgg atcgatccgg
     2221 tactcgatcg tcaccatcac cggcccctgc tcgtgctcga tcgggaacgc cggcaccggc
     2281 tcgtcgaggt agcgcccggg cgtgaggtcg accggctcgc cgagcccttc gtgcaggtgc
     2341 cgcgtggcga acagcgcgac cagcccggtc gccgccgccg cgacgaaact gacgctcacg
     2401 tcggccagcg tggcgacctg cccccacagc gccgcgctcg ccgccgacgc gcccatgatc
     2461 gcgacctggt agatcgacat gccgcgcgcg cgcacccagt tcggcagcac gagctgcgcc
     2521 gacaccgtga gcgtgttgac caccgcgagc cacgccgcgc ccgcgacgaa catcgccggc
     2581 gccgcgagcc acgcgctcgg cgcgaagccg accgcgacca tcgcgactgc ctgcagcacc
     2641 gagccatcgc gcatcagccg gtcgcggtcg aaccgtgcgc gcagctgcg
//
LOCUS       DBNHBY010000835         9060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1081847, whole genome shotgun sequence.
ACCESSION   DBNHBY010000835 DBNHBY010000000
VERSION     DBNHBY010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1081847"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1367)
                     /gene="gspF"
                     /locus_tag="AC16HB_19390"
     CDS             complement(123..1367)
                     /gene="gspF"
                     /locus_tag="AC16HB_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HIR5609010.1"
                     /translation="MAAFRYAAVDSSGKELSGLLEADSARLARQVLRGRGLVPLSVEP
                     VVSEAQRSSVTLALGRRLSQTELAVITRQLASLLGAQLPVADALNVMVEQSEKQQVRE
                     LMAAIRTDVLSGTSLSKALARHPRQFPDIYRALIAAGEESGKLGSVLGSLADYVEERA
                     RLQQKITLAFVYPAIVTLVALAVVIGLLTYVVPQVVQVFAQTKQALPLLTRSLIALSN
                     FVRDYGWIVAVVLFAIGFAINRALRVERIRLRWHRRILTLPVLSVLSRTLNTARFAST
                     LSILAGSGVPMLRALQAAGETVNNLAMRGRVVEATGRVREGYSLARALRQDSDEGKKA
                     GHTRLFPPVLIHLIASGEATGKLPEMLSRAADIHAREAERRALFFTSLLEPFLILTMG
                     VVVMLIVLAVLLPIIEINQLAR"
     gene            complement(1371..2804)
                     /gene="gspE"
                     /locus_tag="AC16HB_19395"
     CDS             complement(1371..2804)
                     /gene="gspE"
                     /locus_tag="AC16HB_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HIR5609011.1"
                     /translation="MATAQRFVPFAFARDNAILLKPASEREAEVWISEATPLAALNEV
                     MRVFPGRVTPIVMPAQQLNDAISQTYAQSEGSAQQIVGDIEGEFDITRMMQEVPDVED
                     LLETEDDAPIIRMINALLTQAARDGASDIHIEPFETYSLVRFRVDGTLRDIVRPKRTL
                     HAALVSRIKIMAALDIAEKRLPQDGRITLRVGGRAVDVRVSTLPTGHGERVVLRLLEK
                     DLSRLELTKLGMSDHTLKQFDALIQLPHGIILVTGPTGSGKTTTLYAAIRRLDHTTTN
                     IMTVEDPIEYDLEGIGQTQVNPKIDMTFARALRAILRQDPDVVMVGEIRDLETAQIAV
                     QSSLTGHLVLATVHTNDAAASITRLIDMGIEPFLLSSSLLGVLAQRLVRRLCPHCKKP
                     EPDGKSYRAIGCEQCSFSGYIGRTGIYELLTVDDEMRLLIHNGANETDIKRFAERRGM
                     AAMRSDAMRWVAAGITSLEEVLRVTRE"
     gene            complement(2808..5288)
                     /gene="gspD"
                     /locus_tag="AC16HB_19400"
     CDS             complement(2808..5288)
                     /gene="gspD"
                     /locus_tag="AC16HB_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HIR5609012.1"
                     /translation="MMRTYTGARTIAVIATTAVLAAVAATDTLVPRAAAQTKPGASSD
                     ETVYLNFPNAELETVVRAVGQFTGRTFVIDPRVKGTLNLVTERPVNKQQAYEQLLAAL
                     RLSGFTIVESGQPGGIARILPEADAKLQGGRVVPPDTAAPRGDQIVTQVFRLQYESAT
                     SLVPVLRPLIAPNNTISAYPSNNTLVITDYADNLRRIARIIEAIDAPANADAEIVTLK
                     HGLAVEIATIATRILDEGARAAGQAIDGGQRVQVMAEPRTNTLIIRTASPARARLARQ
                     LIERLDQPSVTPGNINVIYLRNAEATKLAPLLRAVLASDPSFVPQASTSGLSQTTPMA
                     TGGTPGLGGQPAQPAFQQQPQQVSAGGGTSSALGGLIQPDAATNSLIITAPEPLYRNI
                     RAIIEKLDARRAQVVIESLIVEVSADKAAEFGIQFQDLSGLNSDNTRVVGGTNFNSNT
                     GGSNIIGAAQNLASLGPGLNIGVIRGKVNIPGIGEVTNIGFLARALETNAHANILSRP
                     NIQTLDNEEAQFLVGQNVPFITGSFTQNATGAQNPFQTFERKDIGLQLRVKPQISEGG
                     TVKLAIYLEVSSIVPGTTAGGLITNKRAFTSNLLVEDGDFVVLSGLIEDRSNTSQSKV
                     PLLGDLPLIGNFFRYENRDRSKTNTMVFLRPSIIRSAATATALAADRYDYMQTQIVNS
                     RRPDTLIFRDFNVDPTQGLPARSAAPPTPAPQGAAPIPQSTAPAPAAAPAGAAPATGT
                     FAASASSQPGDRVQLIQVAVLGDIARGRQLQRELRDAGFDAYWESVNSDNGEIVRVRV
                     AVDRTRQDVAQVIAQLKARGLEPILASP"
     gene            complement(5296..6069)
                     /gene="gspN"
                     /locus_tag="AC16HB_19405"
     CDS             complement(5296..6069)
                     /gene="gspN"
                     /locus_tag="AC16HB_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013376.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HIR5609013.1"
                     /translation="MSRFIVYALVGLAAMLAIVTVFAPAQWLAIAVRDASHGHVELAD
                     ARGTIWSGQATVVLGSGAPGGTRASLAEPLSWKLAPWPLLIGEFDLTLSHPSALPEPV
                     VLRTQLMRQVDVSAANLRLPASLLTGLGAPWNTIRPGGIIVLSWDRLHIEAGRLAGNP
                     SAEWQFASSALTPVSPFGHYRVQSSGGYPGTRLNLLTLSGPLEMTGSGTIEEGGRVRF
                     QGIARASPGADPAIKAQLAGLIALLGRRDGDAALLNIGS"
     gene            complement(6097..6585)
                     /gene="gspM"
                     /locus_tag="AC16HB_19410"
     CDS             complement(6097..6585)
                     /gene="gspM"
                     /locus_tag="AC16HB_19410"
                     /inference="COORDINATES: protein motif:HMM:NF016493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HIR5609014.1"
                     /translation="MDALLHWWSERAPREKAILAGGGAVTIAVLIYLLLVEPAWSGIR
                     LLERNLPGVRTQAAQLQGQLAEVGALKARAQVATISATEARSVLEKSIAAAGLKATRV
                     QPLSEGDLQLSFTNAPYAGWSAWLANTERTLGARAISVNATRATAAGNADIELVLRLA
                     RR"
     gene            complement(6589..7842)
                     /gene="gspL"
                     /locus_tag="AC16HB_19415"
     CDS             complement(6589..7842)
                     /gene="gspL"
                     /locus_tag="AC16HB_19415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HIR5609015.1"
                     /translation="MATRTVKERSFERLLLVMPPHRTVASGGNRPQLSSSSVIAYLAL
                     GATGQIERGDAPIPLLPKANRIELVFDCADVFAAEIDAPAISASKLQQALPNLLEDRL
                     LTEPADCHFATGPRDATGKLPVAVIHRALLTRALDALAESGMRPRAAYADLYTVPPPS
                     GQTLAMRIDRGRGTARVGAHEGISFEVERGVPSALPLAIRRLGAKRVRAYGREANKLV
                     ALSDALDTPVEAVGADLDPGAQRDAVNLLQGSYAVGGMLPGMPRITPQSLRMPALWLG
                     VAALAWIAGINAYWFKLQRERSALEQSMQTAFKSAFPNLDPDPSLAVEQTRRELRALR
                     TRAGLTSRTDFSALNAQTAQLLSVAPIGIVTGLDYRDGALRVRFRPGAADAGVQNQLR
                     AQAAPLGLSLRFDADGSARIEPVGG"
     gene            complement(7824..8723)
                     /gene="gspK"
                     /locus_tag="AC16HB_19420"
     CDS             complement(7824..8723)
                     /gene="gspK"
                     /locus_tag="AC16HB_19420"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HIR5609016.1"
                     /translation="MALFVATLATLIVTGLFWNQFIVLRTIENQQLTVQGRMLLAGAL
                     DWARAILREDRNNYDALTEPWAQPLAETRLDQLGETLSLASRATIEGSIEDAQSRMNL
                     RNLVSPDPNIARGELEALRRLCGLLSLPSATADLISVSMVDSGIGLTGVPTAGTEGTI
                     AVRPLPLVLPQDLYGVRGMDPQAAAKLIPYVTVLDVPTPINVNTASAEVIAARVGITL
                     SAARALVAERDRIGYFKDVPDFSTRPQLRGITLDASQLSAQSRYFLIRGKVKLDRAVT
                     RMEALVRRPGVNQVVQVLWQREL"
     gene            complement(8801..>9060)
                     /locus_tag="AC16HB_19425"
     CDS             complement(8801..>9060)
                     /locus_tag="AC16HB_19425"
                     /inference="COORDINATES: protein motif:HMM:NF023044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HIR5609017.1"
                     /translation="GQLTREVSAPIRTPGLLAQAPLTEAKLLANVRAMQVRVWRQGQG
                     WVDAAAGFAPTPGSPTALPDGIEVTLERDGGARIRRVLVTN"
BASE COUNT         1391 a         3079 c         3089 g         1501 t
ORIGIN      
        1 gcatgacggc gagcgcttcg gtccgggcga ctgcatcgtc gcacggccgg gttcgacgca
       61 tctcaagacg cacaccgaaa tgggtgcggt gctgttcttt cgcacgccgt cgcggcggcg
      121 gactaacgtg ccagttgatt gatctcgatg atcggcagca gcaccgcgag cacgatcagc
      181 atcaccacca cgcccatcgt caggatcagg aacggctcga gcagcgaggt gaagaacagc
      241 gcccgccgct ccgcctcgcg cgcatggata tcggcggcgc gcgacagcat ctccggcagc
      301 ttgccggtcg cctcgcccga ggcgatgagg tgtatcagca ccggcggaaa caggcgcgtg
      361 tggccagcct tcttgccctc gtcgctgtcc tggcgcagcg cgcgcgccag cgagtagccc
      421 tcgcgcacgc gcccggtggc ttcgaccacg cgtccgcgca tcgccaggtt gttcacggtc
      481 tcgcccgccg cctgcagcgc acgcagcatc ggcacgccgc tgccggccag aatcgagagc
      541 gtgctggcga agcgcgcggt gttcagcgtg cgggaaagca cgctcagcac cggcagggtc
      601 agtatgcgcc ggtgccagcg cagccggatg cgttcgacgc gcagcgcccg attgatcgcg
      661 aagccgatcg cgaacagcac cacggcgacg atccagccgt aatcgcgcac gaagttcgac
      721 agcgcgatca ggctgcgcgt cagcagcggc agcgcctgct tggtctgcgc gaacacctgc
      781 accacctgcg gcaccacgta cgtcagcagc ccgatcacca ccgccagcgc gaccagcgtg
      841 acgatggccg ggtagacgaa ggcgagcgtg atcttctgct gcaaccgggc gcgctcctcc
      901 acgtagtcgg ccaggctgcc gagcaccgag ccgagcttgc cggattcctc gcctgcggcg
      961 atcagcgcgc ggtagatgtc ggggaactgg cgcggatggc gcgcgagcgc tttcgacagt
     1021 gacgtgccgg acagcacatc ggtgcggatc gccgccatca gttcgcgcac ctgctgcttc
     1081 tcgctctgct cgaccatcac gttcagtgcg tcggccaccg gcagctgcgc gccgagcagg
     1141 ctggcgagct ggcgcgtgat caccgccagt tccgtttgcg acagccgacg gccgagcgcc
     1201 agcgtcacgc tggagcgctg cgcctcgctg accaccggct cgaccgacaa cgggacgagg
     1261 ccgcgcccgc gcagcacctg ccgcgccagc cgcgcgctgt ccgcttcgag caggccggaa
     1321 agttccttgc cgctgctgtc gacggcggcg tatcggaatg ccgccacgcg ttactcgcgc
     1381 gtcacgcgca gcacttcttc gagcgacgtg atgccggcgg cgacccagcg catcgcatcg
     1441 ctgcgcatcg cggccatccc gcgccgctcg gcgaaccgct tgatgtcggt ttcgttggcg
     1501 ccgttgtgga tcagcagccg catttcgtca tcgacggtca acaactcgta gatgccggtg
     1561 cggccgatgt agccggagaa gctgcactgc tcgcagccga tcgcccggta gctcttgccg
     1621 tccggctccg gcttcttgca gtgcggacac agcctgcgca ccagccgctg cgccagcacg
     1681 cccagcagcg acgacgacag caggaagggc tcgatcccca tgtcgatcag gcgggtgatc
     1741 gaagcggccg cgtcgttggt gtgcaccgtg gccagcacaa ggtggccggt cagcgacgac
     1801 tgcaccgcga tctgcgcggt ctcgaggtcg cggatctcgc ccaccatgac cacgtccgga
     1861 tcctgacgca ggatcgcgcg cagcgcgcgc gcgaacgtca tgtcgatctt cggattgacc
     1921 tgcgtctggc cgataccctc gaggtcgtac tcgatcggat cctcgaccgt catgatgttc
     1981 gtcgtcgtat ggtcgagccg gcgaatcgcc gcgtacagcg tggtcgtctt gcccgacccg
     2041 gtcggcccgg tgaccaggat gatcccgtgc ggcaactgga tcagcgcgtc gaactgcttg
     2101 agcgtgtggt ccgacatgcc gagcttggtc agctcgagcc gcgacagatc cttttccagc
     2161 aggcgcagta cgacacgctc gccatgcccg gttggcagcg tcgacacgcg cacgtcgacc
     2221 gcgcggccgc cgacacgcag cgtgatgcgg ccatcctgag gcagccgctt ttcggcgatg
     2281 tcgagcgcgg ccatgatctt gatgcgcgac accagcgccg catgcagcgt gcgcttgggc
     2341 cgcacgatgt cgcgcagcgt gccgtcgacg cggaagcgca ccagcgagta tgtctcgaac
     2401 ggctcgatgt ggatgtcgct cgcgccgtcg cgcgccgcct gcgtcagcaa cgcgttgatc
     2461 atgcggatga tgggcgcgtc gtcctcggtc tcgagcaggt cttcgacgtc gggcacttcc
     2521 tgcatcatgc gggtgatgtc gaactcgccc tcgatgtcgc cgacgatctg ctgcgccgaa
     2581 ccttcgctct gcgcgtaggt ctgcgagatc gcgtcgttca gctgctgcgc cggcatcacg
     2641 atcggcgtga cgcggcccgg aaacacgcgc atcacctcgt tcagcgccgc gagcggcgtg
     2701 gcctcggaga tccacacctc ggcctcccgc tcactggccg gcttcagcag gatcgcgttg
     2761 tcgcgcgcga aggcgaacgg aacgaagcgc tgcgcagtgg ccatcggtta cggcgaggcg
     2821 aggatcggtt cgagaccgcg cgccttcagc tgcgcgatca cctgcgcgac atcctggcgc
     2881 gtgcgatcga ccgccaccct tacacgcacg atttcgccgt tgtcggagtt cacgctctcc
     2941 cagtaggcgt cgaagccggc gtcgcgcagc tcccgctgca gctgccggcc gcgcgcgatg
     3001 tcgcccagca cggcgacctg gatcagctgc acccggtcgc ctggctgcga cgacgccgaa
     3061 gcggcgaacg tgccggtcgc cggtgccgcg ccggccggcg cggcggcggg tgcgggagcg
     3121 gtgctttgcg ggatcggcgc ggctccttgt ggcgccggtg tcgggggcgc agccgaccgc
     3181 gcgggaagtc cctgcgtagg atcgacgttg aaatcgcgga agatcagcgt gtcgggccgg
     3241 cgggaattga cgatctgtgt ctgcatgtag tcgtagcggt cggccgccag cgcggtcgcg
     3301 gtcgcggcgc tacggatgat cgatggccgc agaaacacca tcgtgttcgt tttcgagcga
     3361 tcgcggtttt cgtagcggaa gaagttgccg atcaggggca ggtcacccag caacgggacc
     3421 ttgctctggc tggtattcga gcggtcctcg atcaatccgc tcagcaccac gaagtcgccg
     3481 tcctcgacca gcaggttcga cgtgaatgcg cgcttgttgg tgatcaagcc gccggcggtc
     3541 gtccccggaa cgatcgacga gacttcgaga tagatcgcca gcttgaccgt gccgccttcg
     3601 gaaatctgcg gcttcacgcg caattgcagg ccgatgtcct tgcgctcgaa cgtctggaac
     3661 gggttctgcg caccggttgc gttctgcgtg aacgaaccgg tgatgaaagg cacgttctgc
     3721 ccgaccagga attgcgcctc ctcgttgtcg agcgtctgga tgttcggccg cgacaggata
     3781 ttggcgtgcg cattcgtttc cagtgcgcgc gccaggaatc cgatgttggt gacctcgccg
     3841 atgccgggaa tgttcacctt gccgcgaatc acgccaatgt tcagccccgg cccgagggag
     3901 gccagattct gtgccgcgcc gatgatgttg ctgccgccgg tgttcgagtt gaaattggtg
     3961 ccaccgacca cgcgtgtgtt gtccgagtta agtcccgaca ggtcctggaa ctggatgccg
     4021 aactcggccg ccttgtcggc gctgacctcg acgatcagcg actcgatcac cacctgcgcg
     4081 cggcgggcgt cgagtttctc gatgatcgcg cggatgttcc ggtacagcgg ttcgggcgca
     4141 gtgatgatca gcgaattggt cgcggcgtcg ggctggatca ggccgccaag cgcgctggac
     4201 gtgcccccgc ccgcggacac ctgttgcggc tgctgctgaa acgccggttg cgccggctgc
     4261 ccgccgagtc ccggcgttcc gccggtcgcc atcggcgtgg tctggctcag cccgctcgtg
     4321 cttgcctgcg gcacgaacga gggatccgag gccagcaccg cacgcagcag cggcgcgagc
     4381 ttggttgcct cggcgttgcg caggtagatg acattgatgt tgccgggcgt cacgctgggc
     4441 tgatcgagac gctcgatcaa ctgacgtgcg agcctggcgc gggccggact cgcggtgcgg
     4501 atgatcagcg tgttggtgcg cggctcggcc atcacctgca cgcgctggcc gccgtcgatg
     4561 gcctggcccg cggcgcgcgc gccttcgtcg agaatgcgcg tggcgatcgt cgcgatttcg
     4621 accgcaaggc cgtgcttgag cgtcacgatc tcggcgtcag cgttggccgg tgcgtcgatc
     4681 gcctcgatga tgcgtgcgat gcggcgcagg ttgtccgcgt agtcggtgat gaccagcgtg
     4741 ttgttcgatg gatacgcgga gatcgtgttg ttgggggcga tcaacggccg cagcaccggc
     4801 accaggctgg tggccgattc gtactgcagg cggaacacct gcgtgacgat ctggtcgccg
     4861 cgcggcgcgg ccgtgtcggg cggcacgacg cggccgcctt gcagcttggc gtcggcctcc
     4921 ggaaggatcc gcgcaattcc accgggctgc cccgattcga cgatcgtgaa cccgcttaac
     4981 cgaagcgcgg ccagcagttg ctcatacgcc tgctgcttgt tgaccgggcg ctccgtgacg
     5041 aggttcagcg tccccttcac gcgcggatcg atgacgaagg tgcggccggt gaattggccg
     5101 accgcgcgca cgacggtctc gagctccgcg ttcggaaagt tcagatagac ggtctcgtcc
     5161 gaacttgcgc ccggcttcgt ctgtgccgca gcgcggggca cgagcgtgtc ggtcgccgcg
     5221 accgcggcca gtacggcggt cgtcgcgatg accgcgatcg tccgggcgcc ggtgtacgtg
     5281 cgcatcatcg aattctcagc ttccgatgtt cagcagtgcg gcgtcgccgt cccggcgccc
     5341 caacaatgcg atcaatcctg cgagttgtgc cttgatggcg ggatccgcac ctgggctcgc
     5401 ccgcgcgatc ccttggaaac gcacgcgccc tccctcttcg attgtgccac tgccggtcat
     5461 ttcgaggggg ccggacaggg ttagtaggtt cagccgagtg ccggggtaac ctccgctcga
     5521 ctgtacgcga tagtgaccga acggcgacac gggggtcagc gcgctcgacg caaactgcca
     5581 ttcagcactc ggattgccgg cgagccggcc ggcttcgatg tgcagccggt cccacgacaa
     5641 gacgatgatg ccgcccggcc ggatcgtgtt ccacggggcg ccgagcccgg tgagcagcga
     5701 tgcaggcagc cgcaggttgg cggcgcttac gtcgacttgg cgcatgagct gggtacgcag
     5761 cacgaccggc tccggcaacg ccgaggggtg ggacagcgtg aggtcgaatt cgccgatcag
     5821 caacggccac ggtgccagct tccagctcag gggttcggcg aggctcgcgc gcgtgccgcc
     5881 cggcgcgccg ctgccgagca cgacggtcgc ctgtcccgac cagatcgtcc ctcgagcgtc
     5941 ggccagctcg acatggccgt gcgatgcatc ccggaccgcg attgccagcc actgcgcagg
     6001 tgcaaatacg gtcacgatcg ccagcatcgc ggcgagcccg accagcgcgt agacgatgaa
     6061 gcgcgacatc ggcgtgactt gatccttccg ccgccgtcag cgtcgcgcca ggcgcagcac
     6121 gagttcgatg tccgcgttgc ccgcggccgt ggcgcgcgtt gcgttgacgg aaatcgcgcg
     6181 agctccaagc gtccgctccg tgttcgcgag ccaggcgctc caaccggcgt acggtgcgtt
     6241 ggtgaatgac agctgcaggt cgccctccga cagcggctgc acgcgcgtgg ccttcagccc
     6301 ggcggcggcg atcgacttct ccaacacact gcgcgcctcg gtggccgaga tggtcgcgac
     6361 ctgcgcccgc gctttcaggg cgccgacctc ggccaactgc ccctgcagct gcgcggcctg
     6421 cgttcgcacg ccgggcaggt tgcgctccag tagtcggatt ccgctccagg ccggctcgac
     6481 caacagcaga tagatcagca cagcgatggt gacggctccg ccaccggcca ggatcgcctt
     6541 ctcgcggggc gcgcgctcgg accaccagtg cagcagggcg tccatgggtc agcctccgac
     6601 cggttcgatt cgtgccgagc cgtcggcgtc gaagcgcagg ctcaggccga gtggggcagc
     6661 ctgcgcgcgc aactggttct gcacgccggc atcggccgcg cccggacgga accggacgcg
     6721 caacgcccca tcgcggtagt cgagacccgt aacgatgccg atcggtgcta ccgacagcaa
     6781 ctgggcggtc tgcgcgttca gcgcggagaa atccgtgcgc gatgtcagtc cggcgcgcgt
     6841 ccgcagcgcg cggagttcgc ggcgcgtctg ctcgaccgcg aggctcggat cgggatcgag
     6901 attgggaaac gcgctcttga acgccgtctg catcgattgc tcgagcgcgc tgcgttcgcg
     6961 ctgcagcttg aaccagtagg cattgatgcc tgcgatccac gcgagcgccg cgacgccgag
     7021 ccagagcgcc ggcatgcgca gcgactgcgg cgtgatgcgc ggcatccccg gcagcatgcc
     7081 gccgaccgcg tagctgccct gtagcaggtt gaccgcatcg cgctgcgcgc cgggatcgag
     7141 gtcggcgccg acggcctcga ccggcgtgtc gagcgcgtcg gacagggcga ccagcttgtt
     7201 tgcttcacgt ccgtatgcgc gtacccgctt ggcgccgagg cggcggatcg ccagcggcag
     7261 ggcgctcggc actccgcgct cgacctcgaa cgagatccct tcatgcgcgc cgacccgggc
     7321 cgtgccgcgg ccccggtcga tccgcatcgc aagcgtctga ccgctcggtg gcggtacggt
     7381 gtacaggtcg gcatatgcgg cgcgcggccg catcccactc tccgccagcg catcgagggc
     7441 acgcgtcagc agcgcgcggt gaatcaccgc caccggcagt tttccggtcg cgtcgcgcgg
     7501 accggtcgcg aagtggcagt ccgccggttc ggtcagcaac cggtcctcga gcaggttcgg
     7561 cagtgcctgc tgcagcttgg aggcggagat cgccggcgcg tcgatctcgg ccgcgaatac
     7621 gtccgcgcag tcgaacacca gttcgatccg gttcgccttc ggcagcagcg ggattggcgc
     7681 atcaccgcgc tcgatctgac ccgttgcacc gagcgccaga tacgcgatca cggacgacga
     7741 cgacagctgc ggccgattgc cgccgctggc cacggttcga tgcggcggca tcaccagcag
     7801 cagccgctcg aacgagcgct ccttcacagt tcgcgttgcc ataacacctg gacgacttgg
     7861 ttgactccgg gccggcgcac cagcgcttcc atgcgcgtga cggcgcggtc gagcttgacc
     7921 ttaccccgaa tcaggaagta gcgcgactgg gctgaaagct gagacgcgtc gagcgtgatg
     7981 ccgcgcaact gcgggcgcgt gctgaagtcg ggcacatcct tgaaataacc aatgcggtcg
     8041 cgctcggcga ccagcgcgcg cgccgccgaa agcgtgatgc cgacgcgcgc ggcgatcact
     8101 tccgcggacg ccgtgttgac gttgatgggc gtcggcacat cgagtacggt cacgtacggg
     8161 atcagcttgg cggcggcctg cggatccatg ccgcgcacgc catacaggtc ctgcggcagc
     8221 accagcggca gaggtcgcac tgcgatcgtg ccctccgtgc ccgccgtcgg cacgccggtc
     8281 aggccaatgc ccgaatccac catggacacg ctgatcaggt cagcggtcgc gctcggcagc
     8341 gacagcagac cgcacaggcg gcgcagcgct tcgagttcgc cacgagcgat gtttgggtcc
     8401 ggggatacca gattgcgcag gttcatccgc gactgcgcgt cctcgatgct gccctcgatg
     8461 gtggctcgcg aagccagcga cagcgtttca cccagttggt cgagccgggt ctcggcgagc
     8521 ggctgcgccc agggctcggt cagcgcatcg tagttgttgc ggtcctcgcg caggatcgcg
     8581 cgcgcccagt cgagcgcgcc cgcgagcagc atccggccct gaacggtcag ctgctggttc
     8641 tcgatcgtgc gcagcacgat gaactggttc cagaacagcc cggtgacgat cagggttgcg
     8701 agagtcgcga cgaagagtgc catcagtacg gcggcgcccc gctgacgggg acgcaggcgg
     8761 cgcgcggaac ggcgatcgag cggtgagcgt gtcatcgatg ctagttcgtc acgagaacgc
     8821 gccgaatgcg cgcgccgcca tcgcgctcga gcgtcacttc gatcccgtcg ggcagcgcag
     8881 tcggactgcc cggcgtcggc gcgaacccgg cggccgcatc gacccacccc tgcccttgtc
     8941 gccagacgcg aacctgcatc gcgcgcacgt tggcaagcag ttttgcttcc gtcagtggcg
     9001 cctgcgccag caatcccggc gtcctgatcg gcgccgacac ttcgcgcgtc aactgcccgt
//
LOCUS       DBNHBY010000836         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate MAG05568
            SRR34280926_k127_1082360, whole genome shotgun sequence.
ACCESSION   DBNHBY010000836 DBNHBY010000000
VERSION     DBNHBY010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56415063
            Sequence Read Archive: SRR34280926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:58:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,888
            CDSs (total)                      :: 3,856
            Genes (coding)                    :: 3,823
            CDSs (with protein)               :: 3,823
            Genes (RNA)                       :: 32
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 8 of 33
            Pseudo Genes (incomplete)         :: 19 of 33
            Pseudo Genes (internal stop)      :: 10 of 33
            Pseudo Genes (multiple problems)  :: 4 of 33
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280926_k127_1082360"
                     /isolate="MAG05568"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.939654 N 118.084891 E"
                     /collection_date="2022-05-06"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="AC16HB_19430"
     CDS             complement(<1..1014)
                     /locus_tag="AC16HB_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5609018.1"
                     /translation="MNAAVLNAPADTVAERVDYQTDPSRYRHWRVSYDGPVATVTLDI
                     DENGGIRPGYTLKLNSYDLGVDIELHDALQRIRFEHPEVKSVVVASAKPRIFCSGANI
                     YMLGLSSHAWKVNFCKFTNETRNGIEDASANSGLKFIAACSGTTAGGGYELALACDEI
                     VLIDDRSSAVSLPEVPLLGVLPGTGGLTRVTDKRRVRRDHADIFCTVAEGVRGSRAKD
                     WRLVDHLVKPQQFGEFVRKRALELAESSDRPADAQGIALQPLARTIDAGGYRYEFVDV
                     QLDRAARRATITVRAPSNPEPASLDAIVAAGCRWWPLQMARELDDAILSLRTNELDLG
                     LWVL"
     gene            complement(1028..1927)
                     /locus_tag="AC16HB_19435"
     CDS             complement(1028..1927)
                     /locus_tag="AC16HB_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5609019.1"
                     /translation="MVIEAPLPRPPLAPAGNADAESAFLQSLGRRVRELRDRRGLSRK
                     QAARESGVSERHLAHLEAGEGNISILLLRRIVRVLGVSLADLLSNHDDESEERRLIRR
                     LLERVPADRLEDVAFRLLRDFGDEGSQRRRRIALIGLRGAGKSTLGPRLAHELGCPFL
                     EMDREIERDAGMPVAEIFSLYGQAGYRRFEKRALERLVNEHDRAVLSVGGGIVSQPDT
                     YAYLLANCHTVWLKAAPEEHMARVIAQGDLRPMAGNEEAMEDLRRILKAREALYAKAD
                     AVVDTTGISLETSFARLRQAVLI"
     gene            2095..3672
                     /locus_tag="AC16HB_19440"
     CDS             2095..3672
                     /locus_tag="AC16HB_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HIR5609020.1"
                     /translation="MKLENLIAGRWQQGDDDGAPLVDPVTGHVIAIAASAGADLAAGL
                     AYARATGGAALRALTYAQRANLLTAVADTLTKHRDAYFDIARRNSGSPKADAAIDIDG
                     SIYTLKTYARLARALGDARRLAEGAPHPLSKDGAFQARHYLVPRTGAAVLINAFNFPA
                     WGLWEKAAPALLAGMPVFAKPATPTAWLAQRMVRDLVDAEVLPEGAISIVCGGARDLL
                     DHVNETDVVSFTGSADTAEKIRTHPAIVRNSVAVNVEADSVNAAILGVDARPGSREFA
                     LLVAEVVREMTQKAGQKCTAIRRVLVPAAVAKSVTDAVAAELAKVRLGNPIIADVTMG
                     PLINHAQRFAAYEGIAALMNECQRVTGDPTHLSLIDADPAVGAFVPPTLLACSQPANA
                     RRVHEVEVFGPVATVIPYKSIDDAIALARRGRGSLVASIFSSDAAFLAQVIPGIADLH
                     GRILVVDSAVDSKHTGHGNVMPTCTHGGPGRAGGGEELGGLRALAFYHRRVALHGSAA
                     RHEALGRDAADAAALWS"
BASE COUNT          606 a         1314 c         1320 g          589 t
ORIGIN      
        1 cagcacccac agcccgaggt cgagctcatt ggtgcgcagg ctcaggatcg cgtcgtcgag
       61 ttcgcgcgcc atctgcaacg gccaccagcg gcagccggca gcgacgatcg cgtcgagcga
      121 cgccggctcg gggttcgacg gagcgcgcac cgtgatcgtc gcccgccgcg cggcgcggtc
      181 gagctgcacg tcgacgaatt cgtagcgata gccgccggcg tcgatcgtcc gcgcgagcgg
      241 ctgcagcgca atgccttgcg catccgccgg ccggtcgctt gactcggcca gctcgagcgc
      301 acgcttgcgc acgaactcgc cgaactgctg cggcttgacg aggtgatcga ccagtcgcca
      361 gtccttcgcg cggcttccgc gcacgccctc ggccacggtg cagaagatgt cggcgtgatc
      421 gcgccgcacg cggcgcttgt cggtcacgcg cgtcagcccc ccggtgcccg gcagcacgcc
      481 gagcagcggc acttcgggca ggctgaccgc cgagctgcgg tcgtcgatca gcacgatctc
      541 gtcgcaggcg agcgcgagct cgtagccgcc gcccgccgtg gtgccactgc aggcggcgat
      601 gaacttcagt ccggagttcg cgctggcgtc ctcgatgccg ttgcgcgtct cgttggtgaa
      661 cttgcagaag ttcaccttcc acgcgtgcga cgacagcccc agcatgtaga tgttggcacc
      721 cgaacagaag atgcgcggct tggcgctcgc gaccaccacg ctcttcacct cgggatgctc
      781 gaagcggatc ctctgcagcg cgtcgtgcag ctcgatgtcg acgccgaggt cgtacgaatt
      841 cagcttcagc gtgtagccgg ggcggatgcc gccgttttcg tcgatgtcca gtgtcaccgt
      901 cgcgaccggc ccgtcatacg agacgcgcca gtggcggtag cgcgaaggat cggtctggta
      961 gtcgacgcgc tcggcgacgg tgtcggccgg cgcgttcagt acggcagcat tcatgaggtt
     1021 ctccagttca gatcagcacg gcctggcgca gacgcgcgaa gctcgtctcg aggctgatgc
     1081 cggtggtgtc gaccaccgca tcggccttcg cgtacagcgc ctcgcgcgcc ttcaggatgc
     1141 ggcgcaggtc ttccatcgct tcctcgttgc ccgccatcgg ccgcaggtcg ccctgcgcga
     1201 tcacgcgcgc catgtgctcc tcgggcgccg ccttcagcca cacggtgtgg cagttggcga
     1261 gcaggtacgc gtacgtgtcg ggctgcgaca cgatgccgcc tccgaccgac agcaccgcgc
     1321 ggtcgtgttc attgaccagg cgttcgagcg cgcgcttctc gaagcggcgg tagccggcct
     1381 ggccgtacag cgaaaagatc tccgcgaccg gcatgccggc atcgcgctcg atctcgcggt
     1441 ccatctcgag gaacgggcag ccgagttcgt gcgcgagccg cggcccgagc gtcgacttgc
     1501 cggccccgcg cagcccgatc agcgcgatgc gccggcggcg ctggctgccc tcgtcgccga
     1561 aatcgcgcag cagccggaac gccacgtcct cgagccggtc tgccggcacg cgctcgagca
     1621 atcggcggat caatcggcgc tcctcgctct cgtcgtcgtg gttcgacagc aggtccgcca
     1681 gcgacacgcc gagcacacgc acgatccgtc gcagcagcag gatcgaaatg ttgccttcgc
     1741 ctgcttcgag gtgcgcgagg tgccgctccg acacgcccga ttcgcgcgcc gcctgcttgc
     1801 gcgacaggcc gcgccgatcg cgcagttcgc gcacgcggcg accgagcgac tgcagaaagg
     1861 cagattcggc atcggcattg ccggcaggtg cgagcggcgg tcggggtagc ggagcttcaa
     1921 tgaccacagg aactttcttg catcttcaat tgcagggccg cgcagaatag tgctcgaatt
     1981 tgatccgagt caagggaaaa tcccgtcaag acggaggggg atgatgcagt aaattgcttg
     2041 acgcgtcggg ctcggcaggt agcattcgcg cactcggaag cggaaggaag acgcttgaag
     2101 ctggaaaacc tgatcgccgg tcgctggcag cagggtgacg acgatggcgc gccgctggtc
     2161 gatccggtga ccggccacgt gatcgcaatc gccgcaagcg cgggcgccga tctcgccgcg
     2221 gggcttgcgt atgcgcgcgc gaccggcggg gccgcattgc gcgcgctgac gtatgcgcag
     2281 cgcgcgaacc tgctcacggc ggtcgccgac acgctgacga aacatcgcga cgcatacttc
     2341 gacatcgccc gtcgcaacag cggctcgccg aaagccgacg cggcgatcga catcgacggc
     2401 agcatctaca cgctgaagac ctacgcacgt ctggccaggg ccctcggtga cgcgcgacgg
     2461 ctcgcggagg gcgcgccgca cccgctgtcg aaggacggcg cgttccaggc acgccactat
     2521 ctcgtaccgc gcaccggcgc ggccgtgctc atcaacgcgt tcaatttccc cgcctggggc
     2581 ctgtgggaga aggccgcgcc ggcgctgctc gccggcatgc cggtgttcgc aaagccggcc
     2641 acgccgaccg cgtggctcgc gcagcggatg gtgcgcgacc tcgtcgacgc agaagtgctg
     2701 ccggagggcg ccatctcgat cgtgtgcggc ggcgcgcgcg acctgctcga ccatgtgaac
     2761 gaaaccgacg tggtgtcgtt cacgggttcg gccgacacgg cagagaagat tcgcacgcat
     2821 ccggcgatcg tgcgcaactc cgtggcggtg aacgtcgagg ccgacagcgt gaacgcggcg
     2881 attctcggcg tggacgcgag gcccggctcg cgcgaattcg cgctgctggt ggccgaggtc
     2941 gtgcgcgaga tgacgcagaa ggccgggcag aagtgcaccg cgatccgccg cgtgctcgtg
     3001 cccgccgcgg tcgcgaagag cgtgaccgac gcggtcgcag ccgaactggc gaaggtgagg
     3061 ctcggcaacc cgatcatcgc cgatgtcacg atgggaccgc tgatcaacca cgcgcagcgc
     3121 ttcgcagcgt acgaaggcat cgccgcgctg atgaacgaat gccagcgcgt gactggcgat
     3181 ccgacgcact tgtcgttgat cgacgccgac ccggcggtcg gcgcgttcgt gccaccgacg
     3241 ctgctcgcct gctcgcagcc ggccaacgca cggcgcgtgc acgaggtcga ggtgttcggc
     3301 cccgtggcca ccgtgatccc gtataagtcg atcgacgatg cgatcgcgct cgcacgtcgc
     3361 ggccgcggct cgctggtggc gtcgatcttc tccagcgacg cggcgttcct ggcgcaagtg
     3421 ataccaggca tcgccgacct gcacggtcgc atcctggtcg tcgacagcgc ggtcgacagc
     3481 aagcacaccg gccatggcaa cgtgatgcct acctgcacgc acggtggtcc gggccgcgcc
     3541 gggggcggag aggaactcgg cggcctgcgc gcgctcgcgt tctatcaccg ccgagtcgcg
     3601 ctgcatggct cggccgcgcg tcacgaggcg ctcggacgcg acgccgccga cgcggcagcg
     3661 ctgtggagtt gagcgaatga cgcccgaaca gttcacggca ttgatcgcgc aggtcacgtc
     3721 gacgctcgtt ggtaagccgc tcgacgccgc actcgaggcg gaactgaacg cgcgctgccc
     3781 ggcgggcagt gcgaccttcg aggcgatccg cgctgcttgc agggacgcg
//
